LOCUS       DAOPAI010000001        80516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_344_length_80516_cov_5.458433, whole genome shotgun sequence.
ACCESSION   DAOPAI010000001 DAOPAI010000000
VERSION     DAOPAI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 80516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 80516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_80516_cov_5.458433"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKV49_00005"
     CDS             <1..553
                     /locus_tag="PKV49_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22044.1"
                     /translation="NQIFSTSTTPPTGYTRRNILLGENDCSDNHKWARPGASLYSAAS
                     FVNQVDVGLDYDWNCDHNEEVDPRQDLSCNSTSTYSRYQYKLCKDYDTGGCCGSGGGG
                     NTKAPNFQFARIAQADDVACCNGRQTLTGCFLSNTKSIGSDSCGQERDFAWDTSVMPE
                     NSTSCVGGRLNFQVYSYGKIGCR"
     gene            563..1630
                     /locus_tag="PKV49_00010"
     CDS             563..1630
                     /locus_tag="PKV49_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPL22045.1"
                     /translation="MTKATATKSLRINITTVTIVKTVAILLLLYLAFLVKEILALVFV
                     ALIFASAFDPWVDFLQRRKWPRTLSISLVYLIVLSLVTAMIYVMVPILVKEATDLAAS
                     FPYYFNKLSEILNNLRQVFGNYGLADNFQSNLDNLAATLSAWILQVVSAVYGAFNGLF
                     SVFLIMVLTFYMVVEENAIKKIVWSLTPARHQAYAIDLVGRMQLQIGYWLRGQLILCL
                     AIFLLTYLGLSILGVKYALLLALVAGLTEAIPYLGPILGGVPAVLLAMTQSPRLALVV
                     IVMYLIIQQLENNILVPQIMKRAANVNPITSIIVLLVGYKIGGVAGAVIAIPVATAVS
                     VGLKDLFNYKEKKELRLDKAA"
     gene            1643..2632
                     /gene="trpS"
                     /locus_tag="PKV49_00015"
     CDS             1643..2632
                     /gene="trpS"
                     /locus_tag="PKV49_00015"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPL22046.1"
                     /translation="MKTILTGDRPTGQLHIGHLFGSLLNRVKLQADYDTYIMVADVQA
                     LTDNFNYPDKVRHNVYEVVLDNLAVGVNPHQATILIQSMVPEIAELTVFFSNLVTLAR
                     LERNPTVKTELEQKKALFKGGVTYGFLGYPISQAADILTFQADLVPVGQDQEPMLEQT
                     REIARKFNSIYGRLFPEPRALLGDFPRLSGLDGQAKMSKSLGNAIYIADEPAVIDKKV
                     KAAKTDPQKIRQNDPGHPPICPVFGYQQIFNAAEAKNIAKECKAGRLGCVDCKRCLAE
                     TITAYLAPFQERRRAYAGDMPKVRDIVRDGTAKARQRARQTMAEVREKMKIDY"
     gene            complement(2634..2879)
                     /locus_tag="PKV49_00020"
     CDS             complement(2634..2879)
                     /locus_tag="PKV49_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HPL22047.1"
                     /translation="MPSLKYLPGTLPRKKLTTALEKLGFTINKDGGKGSHYKATYQIT
                     QKSITIPKRLNKVVLFYLLKEIERISGISWEDIKREL"
     gene            complement(2866..3189)
                     /locus_tag="PKV49_00025"
     CDS             complement(2866..3189)
                     /locus_tag="PKV49_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22048.1"
                     /translation="MSIIKQAKDLAAKMTSGRNMPTGLAEMYRYFKNNGPIKFNQYRK
                     NGLIVAVSSNFIYGSIITSGKDTGELDKNIKDAILTSFEIPSTYAKEAGLYQTGDKER
                     EYAIA"
     gene            complement(3257..3799)
                     /gene="pth"
                     /locus_tag="PKV49_00030"
     CDS             complement(3257..3799)
                     /gene="pth"
                     /locus_tag="PKV49_00030"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPL22049.1"
                     /translation="MKKIIVGLGNPGAKYAKTRHNAGWLLIDRLAAELASETGQKISW
                     QNKKNWSAEVASIGDTLLVKPQTMMNDSGRAVSAIINYYNLLANDLSDILLVIHDELD
                     LPMGAARWSTDSRSAGHNGVQSIIDYLGTQKFNRYRLGIQPTTKPTNTVDYVLKNFSA
                     AERKNLQKTDLTEVKRFIND"
     gene            complement(3805..5274)
                     /gene="der"
                     /locus_tag="PKV49_00035"
     CDS             complement(3805..5274)
                     /gene="der"
                     /locus_tag="PKV49_00035"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPL22050.1"
                     /translation="MPPIKPSQIIIFGRTNVGKSTLFNRLVGAPRALVADLDHTTRDA
                     NHHIISWNRRRLELVDTAGLNELIYLTISPKKSKQLTELEEKIQYQTGQLLKKAAVVL
                     FVVDGQAGLLPPDRELALACKKLLPKETPILLVVNKIDSPRHRDKISEFYTLGWGEPW
                     PVSAATGMGTGDLLDEIVKQVNRQRRNAKNKLSAPHDSLPDATNENELELVAYAEQPD
                     YKVCLLGKPNVGKSSLLNALLGYNRVLVSAIPHTTREPQDTFIKYRNKIIQIVDTAGI
                     ARKDPGKKKPLEKMGMAKSRQRLNQADIAILLVDISQPISHQEASLAEEIINRKKSLI
                     IAANKWDKVEERDTVKFTEAIYHRLPFVGFAPIAFVSAKTTSKVQKILDLIIEVGAQR
                     SISLSKSQLDHFLSRIVKHHRPAKGKGLKHPHIYSFVQTEAGPPCFTVTIGIKDNLHF
                     SYLRFIQNQLRHTYGFIGTPISIHVARGRQINPQHKSNH"
     gene            complement(5279..6961)
                     /locus_tag="PKV49_00040"
     CDS             complement(5279..6961)
                     /locus_tag="PKV49_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPL22051.1"
                     /translation="MPNQNNDNQDILAELFDDQPEYPTEFQPPMPTSRASEPIRIVVE
                     PANKQPQPANHWRNERATVGLQSSAIERPRPRKKRFSCLFLVLSLVLLAYLGWRIWPA
                     TQSGGAANSQGFSFWKSLSMLTKFKLGVSQRTILKGEDQDRINFVLLGIGGKNHDGGM
                     LTDTIMVASFQPSTHKVVMMSIPRDLTVPMTGYGWRKINSANSLAEAQNPGSGGQATA
                     DLISEILEMPIHYYLRVDFDGFVKIIDDLGGIEVEVENTLDDYRYPIMGRENNPDYYS
                     RFEHLHIDAGRQTMDGQLALKYARSRHGVGVEGSDFARSKRQQKILEAVKDKLFSANT
                     LFKPKLIMNIIGNAEEHVTTNLQPDELVSVWQLAKDVNQSQITNIILDNGPKGLLRDV
                     IGTDGAYILTPVTGDFSQIISRFNESFTVAAPSLVDNNSAITKTTATVATISPDNLPN
                     KAEAKLDIRNSTTINGLAGRTKTRLQQYGFTVTNISNTQHKNLAQTTIYDLTYGAKPA
                     DLALLQHKTKAIIAADIPQWLATETSQLIKQNPNIAQPDFILLLGLDYSNNP"
     gene            7131..7715
                     /gene="lepB"
                     /locus_tag="PKV49_00045"
     CDS             7131..7715
                     /gene="lepB"
                     /locus_tag="PKV49_00045"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPL22052.1"
                     /translation="MIFTYDFKARLRLWGISLLEIVKVGLLALAIVLPIRLFIVCPFY
                     VKGASMEPNFYDKEYLLIDEISYRFQAPQRGEVVVFRYPQDPREYFIKRIIGLPGETL
                     KMDQGDVYLLDKSTNEWTKITETYLPSTDQTFALDSNELTLGPDEFFVLGDNRAHSRD
                     SRFFGPLNRRYIVGRVMLRGLPISRAQIFKTPSY"
     gene            7752..9089
                     /gene="hisS"
                     /locus_tag="PKV49_00050"
     CDS             7752..9089
                     /gene="hisS"
                     /locus_tag="PKV49_00050"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPL22053.1"
                     /translation="MVEKNKTTVARPVKHEAAPSSFRRLKGMKDVLPGDYPVWDFVLR
                     TAFRLADFHGFERVDTPVLENLSLYERSSGRTSDIVTKEMYSFVDKSGDRIALRPEAT
                     PGVVRSYIEHGWLNLPQPVKTLWYGPLFRHDKPQLGRYRQHHQINFDIFGEASPLADV
                     QVIFLAHQIVSDLGIKAQVDINSLGCSDCRPNYINKLLAYYREKKHRDQLCQDCRKRL
                     LKNPLRVLDCKEPACINVAREAPQMVDYLCDPCKQHFVKILEFLDGLSVPYNLNSQLV
                     RGLDYYTRTVFELVVPSQTAERPLSLGGGGRYDQLVEYLGGRPTPACGFALGVERLIL
                     QLDENHVKVATSQDQPDIFLAQLGDGARLKSLLLFEELRQVGLTIRQSFTKDSLKAQL
                     ALASQVGAKYTLIIGQKELLDGTILLRDMESGSQEVLDFKKVKTIMAKRLGKN"
     gene            9131..9364
                     /locus_tag="PKV49_00055"
     CDS             9131..9364
                     /locus_tag="PKV49_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPL22054.1"
                     /translation="MVEFKRKKGESFESFLRRFNKRLQLDGKLMLVRQNRYHKNEPNK
                     RQVRLSALVRKELREKRAYLRRIGKLKDEFRQW"
     gene            complement(9415..10917)
                     /locus_tag="PKV49_00060"
     CDS             complement(9415..10917)
                     /locus_tag="PKV49_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPL22055.1"
                     /translation="MTIEQIIAKIADHYPPADIALIKKAYNFAVKAHDGQKRLTGEPY
                     IQHPLHTAFILAQLNADAPIVAAGLLHDVPEDTKYKLPTIKKLFGEDIAFLVGSVTKL
                     SLISYRGQERYRESLKKMFLAMAKDLRVILIKFADRLHNLRILEYLPEEKRQRIARET
                     LEIYVPIAGLLGIWHLKWQMEDICFRHLHPHEYQELLQKYEVEKKLELAQYISRAGRE
                     LGTALDELKINHEINGRFKHLYSIWQKMNNKQRKFDEIYDVFALRVIVPTSDDCYRTL
                     GVIHSLWKPNNNRFKDYIALPKPNGYQSLHTTVFGEEGRATEFQIRTPQMHEQAQYGV
                     AAYWNYKEGNKYQLTPNLHWTQEILALQKDNPDTQTFIQKLQLDIFNDHLFVLTPRGD
                     VHELPLGATPIDFAYAVHTEIGNKAKGVLINNKIGRLDEILHNNDLVEIIIDKKQAGP
                     DLAWLKFVKTSKAKAAIRQYAHRSALVRLAKLQSLRDWWKQREQKKKGQN"
     gene            11000..13576
                     /locus_tag="PKV49_00065"
     CDS             11000..13576
                     /locus_tag="PKV49_00065"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22056.1"
                     /translation="MKRLSFSVTWLLASVCLLIICLTPLAFGVPNVLAEDSLGVGYMN
                     DTGLSAQDPRITVARIIQIALGFLGTIAVVLVIYAGFLWMTSEGNTDKLKRAKEILKS
                     AAIGLMIILSAFAIVTFIIDKLNDALGHGSGQSVVDRPSDETGGLGALGSGIIQSVYP
                     APGQTDVPRNTGVIVTFREKVKASTVCQGGNDSTGVCNNANIVTSSIRFFQTEAGDNE
                     ATNLAADKVKVSSTDGKTFVFAPTEYLGSASTNIWQSVKLTTSVRKANGQPAFSLGDF
                     VWQFEISTRVDLTPPQVQLGGVTPFPDNSQDTNHGVQLAQPATAVIDVLLQPQTAAAA
                     TAGTPEPLGSSGAARLEGTYNCSGSGTIRVVASGTSPVKAQVQGGIAGLMDDNDVSDG
                     RVSLGCGLTLIKNGSIAPDNHETIAAGNIWDIEVAAARSGDSLTVGNQSYIFVNSTST
                     HPNHIVVASSLSATADRIVERINQTNSDVSAARQSASNNKIVLQARFAGEGGNSLVLL
                     DNSLGISIESGFSGGQNRQEQITVNGLNDSPRNSIIQINFSEPVSPLTLVGTSQEVSS
                     TIEVINVNNNSPVSGRFVISNQYQTLEFIPSEECGTNACGDLIYCLPANSQLLVRIRA
                     ANLQPCAASADCLDKTPYNQCSGSCQKTADDHYPLAALPLTSGLVDASFNSLDGNRDT
                     KVVGPVNFYSENTQDTNQRDSYSWSFFVNDDIDLTPPRLTAGSLSPSLGLATSSLNQL
                     IEFSFNKLMMISTLNSGEVLINNHNHRLINLFSTGLAPGYWLTSRAVDFGVPDGYPDF
                     TTVTVNHDLLADATRYSVEIGSGVKDLRQNCFKPCQDDAACSATDASCCNGSPSSGAT
                     CN"
     gene            13589..19372
                     /locus_tag="PKV49_00070"
     CDS             13589..19372
                     /locus_tag="PKV49_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPL22057.1"
                     /translation="MTRGQKFLLSGLLIGGLAVAVWWGGQTVLAQTTTDLGVDYVAAS
                     GLSAQDPRLTVAKIIRIAFGLLGTIAVVLVIYAGFLWMTAAGNQDKIQRAKDILKAAV
                     IGLAIILSAFAITNFIINQLLEATNGPGGSGSVDPEIIYPPADADFMVTGSSPANGSI
                     DLPRNTLVRFQFNGSINANSVNAGTAQILADGSPVAGSWQVNGQEMFFQPDAICNGSC
                     GEVNCLPENGTISVELTSGIQSAGANPKNLVCGSAIGSATCSLNFAVGSTIDCQDPTA
                     EFISSVGICSGHNNNIRVAASDDSSLARFEFLANNQSFNLQTGNFGTSFVASALWTPT
                     SSAGANFTLQALVDDLAGRRTSVQQAGKLLPAHCCNQTQDGDETGIDCGGSCLGCQGA
                     ACATDLQSPAATCNNNLCSSGFCTQQGSNASACQAAGYAAGTASCCLCKNKPIIASLS
                     PQGGFCVNNTDQYCLINSDCGTGGVCDQNSPNAKTGNLMTIIGSGFGTTTGSVVFADN
                     KQGDLLVCGVNSWSNNQIVVKVPTGAITGPVKVTNSDNFSATSTQSVVINNIARPGLC
                     SLSKPTGTTNELINYQGLNFSNSGPAVTKALYGNLTNPIPTNESQVLSNVLIKARVPS
                     LQPGQVTTFVRNEGTTIGPASNYLSFNKLADPGSQPQITGFEPSSGPPGQYVTIYGQG
                     FGNLRAGSLVKFGSIEANYEFPAECANSVWSDRQVIVKVPNNIAVGTYNLSISLASGA
                     VLAATDVFTVNTNPLAPSICRISPTTARVGSQLQVWGEYFRDYDAATSRLRFSQNINA
                     TSSWFYQSSDNFGSASSTIPVGAVSGPVRVVNANGVSNGLNLKIGACQIDSDCQGQIC
                     CPASSPRGGQCITGGQPACYGTSASCVYRWSFNTGSGNQACQDGTTLCGNQCCDSSQE
                     CQDNVCVNKALLSCNDYHQCNGALYCPNSPGWCSQKLHPTINGGCGDDYCRGLFVEQG
                     LSPDSVNYWANANVCVNQQECDPAKAVVFQVGVNQVTYQLHCQDGYWTTNLTAGQCPP
                     GQTDYGQWQLLANNICRSNRPCSQCANHLSCLTLQNQSYCGLDQKVCASGFTCQDNQC
                     QKAADSCECCCDKSLNNPISKINPGCCSPLTCTDTCGAGDVQVSIDGVTTTKQFGVCS
                     GCKEVSGGQAERDAACNCTGSTGKYCDTSTPLGTCRDCSQIANAAECASRASCCFDQK
                     TNKCRGVVNNAKIISGADAGKCGYYHCDGQACAAELTASGQFATATECETKCRVVSLQ
                     GQSCNLATTTIISLNPYNNLPNTEQIGCGYQLCAAPLSCRVASGTSTATNNPADCGIC
                     CCDPSHDTCAQLSTNLSCVANRGQCSGSGRGLCCGCQSDVDCGDNLNNGCGRDTCCQA
                     RPMLVDTLPQNGDNGVCRNAVIQASFNQAVDLASLSDNLLLIADYGNNRECPDGTKYL
                     AMDSAKPISWLARLRQRILAWIKPSALAVLSTAGNTYCSINREISVFNQDVIIRPTSV
                     LDMNRRYFVVMVTDADINDNRPAGILNQAGIGMSRGQPLRFNNLQFPAAIQWSFTTGK
                     HICQIDHVKIDPNSVLLSNLEDSANLNATAISANGQPLATSTNYNWQWSWLVGNQSVA
                     QLDSADNKAIVRPQNKKDAETYVRATAQVTADHSTGAGTSQTSFSQEAAIKVFLCANP
                     WPARRSDGSWRPWTDYATNMQFYYCRDGQANTQSSDLPGLKVVSSTDPTAINPAFYFF
                     RYNQATTSPVNLQGTASPAGQSVSLTWNAYPSAVGYKVYYGLRSGQYFSDPINVSQDT
                     LTTTVNDLINGQLYYFAVSAILQIDQATFVETPLSAEIQVRPLDITAPAAVRCVVQNI
                     VNNNINLEWRRNTDDTVAYRIEYRASGATAAGKVIIINQPASGRTVHGVIPDLVNPEN
                     VLIDIKAVDLAGNISARQNESLVGCFN"
     gene            19438..20181
                     /locus_tag="PKV49_00075"
     CDS             19438..20181
                     /locus_tag="PKV49_00075"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22058.1"
                     /translation="MFDEVNNQPTPASASQPTDNSMPNQAPAAPTNQATDGQVQDIFA
                     NGESPAANQPSGQPNFSQPFMPAGLPVTAQPVNQLVPKSGSNAKLILVVLVILILAGA
                     AALVYLQYFKKTPPPIITPEAINVNSETEVTTTDEAMATATDETIVSPTSSDNPVGVD
                     IDTINVFDENVSSTELDQTTIIDSSMPEPDSEAIANLDSDQDGLTDAEEINIYQTNPL
                     QADSDGDGYTDGDEVRAGYNPLGSGKLVQ"
     gene            20207..21559
                     /locus_tag="PKV49_00080"
     CDS             20207..21559
                     /locus_tag="PKV49_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22059.1"
                     /translation="MSDQPTKNFKIAYSDETGVSTDQIDFVKQSDVGVGIEAGQYIYT
                     MPKAFRFGPKVKTNNKVGILVMVAGLILFVGLVYVGYLMLSKKPIFSFRPVASDNNPT
                     PISVDNQAPTTTTTTEPITEVAIDLSDPEQAYLQLRLKLDQAATLEEYLNSFIAGASQ
                     NKAYQLAEQTIGLDALSDSQKSSTLSISKSMLVPLSSADQITKDISGDRAVLTITKAD
                     TGRIAIVTMLLENDQWKFDDEHWQDAVNLSVSTTTDNLIIPSSSSSTIDLVEEFSLDA
                     DTDQDGLTDKEEIILGTKIDQADSDNDGYDDLTELRAGYNPAGSGKITNNSGLKVVKQ
                     GRWSTIVPAAWTSQLGRDESAIFRTDDGHFIQAVILTKKNGQDLADWYQEIFTGQTAD
                     VVNVGRNQLAISLDGLTYYIGRPDLNYLVSLTYNPETTKLLSYSNIFRLAADNMTLSD
                     "
     gene            21562..21969
                     /gene="ybeY"
                     /locus_tag="PKV49_00085"
     CDS             21562..21969
                     /gene="ybeY"
                     /locus_tag="PKV49_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPL22060.1"
                     /translation="MVTACNLTKQNIDLDRWQKISQLFLQEQQADGDISLVLVGDRRI
                     RHLNNQYRHRDQTTDVLSFTGDGDGQLGEIMVNYQQITRQARRFGHSTWRELIFITTH
                     GILHLLGYDDDTDGGWQVMEKISQEFVEKYKIC"
     gene            21963..22343
                     /locus_tag="PKV49_00090"
     CDS             21963..22343
                     /locus_tag="PKV49_00090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPL22061.1"
                     /translation="MLSYRRLLKSFRYARHGLWQVAKEEQNFAIELTIGVSAIILAGL
                     LKFALWEWAILSLTIGFVLITELFNSAVERISDLLKPRLDIYVKEIKDIMAAAVFLAA
                     GLSVIVGLILFLPHLIKLLITIFQ"
     gene            22349..23602
                     /gene="ftsA"
                     /locus_tag="PKV49_00095"
     CDS             22349..23602
                     /gene="ftsA"
                     /locus_tag="PKV49_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPL22062.1"
                     /translation="MRDQTLVAGLDIGSTEIRLVVGQTTGGQLQIIGAVSAPAAGISK
                     GIVNNIEEVASSVMACLEKSERLIGVRVDSVYIGVSAPSLKCERSKGVVAINQMNNEI
                     TDDDVTRVIEAAQALSLPANYEVLHVIPIKYRVDNQDDVKNPVGMSGIRLEVEVLIIQ
                     SLTNQIRNMTKAIEQAGLSIVSPVLAPLAAAEAVVSARSKELGVALVNIGSLTTSLAV
                     FEEGQLLHAAILPIGSDHITADIAIGLRCSINLAEKIKLEFGQANPEKIDKDDEVDIT
                     PLISPDNEVDEPMTVSMKYLSGIIEARVSEIFHKVDVELKKINRSGMLPAGVVLLGGG
                     AKLAGLTDLAKKNLRLPVCLGANRNITAVIDKVNDLSYLTALGLVAWGDHYDFLTDND
                     SVSSGFKPKQVIKRAKDWFGSLFLK"
     gene            23686..24894
                     /gene="ftsZ"
                     /locus_tag="PKV49_00100"
     CDS             23686..24894
                     /gene="ftsZ"
                     /locus_tag="PKV49_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPL22063.1"
                     /translation="MTEIKPAVEAPAKIKVVGVGGSGGNAINRMIEGGIKGVEFIVIN
                     TDIQDLNYNKAPIKIHIGKTVTKGLGAGMNPDLGRDAAAESQNEIRDALKGADMVFVT
                     CGLGGGTGTGASPVVAELAHDLGALTIAVVTKPFTFEGAQRRKIAERGQAELTDKVDA
                     VITIPNDRIFQVIDKKTTILEAFGIVDDILRQGVQGISEIINVPALINVDFADVKSIM
                     MNAGSALMGIGQASGENRAIEAAKAAINSPLLEMSIDGAKGVLFTITGGPSLALDEIH
                     EAAKIITNSVDEEARIIFGTSVDNNFKDEVRITVIATGFDSRSASNDNQAIGKSYNHF
                     YKPTTFVEKGQDDDANEEVKENRKGLMSALKKAQSEPARQPTPPAASSSSVDEDDDLS
                     IPTFIRKKLL"
     gene            25077..25532
                     /gene="nrdR"
                     /locus_tag="PKV49_00105"
     CDS             25077..25532
                     /gene="nrdR"
                     /locus_tag="PKV49_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPL22064.1"
                     /translation="MKCPICYHSETKVVDSRVASDGLSIRRRRECLKCGFRFSTYEET
                     EILDLLVIKRDGRKEVYSRDKMISGLRKATEKRPINSDQFKQLVNLIERDIQAARQPE
                     INSQDIGRIVMKQLKKIDKVAYIRFASVYSDFELQDLSQELNRLLKLKK"
     gene            25545..27587
                     /locus_tag="PKV49_00110"
     CDS             25545..27587
                     /locus_tag="PKV49_00110"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPL22065.1"
                     /translation="MSQGISKICKRSGEIVDFDQSKITNAINKAMSAVGQPDKAKAEI
                     ISNEVVKLINQKFHERSIPAVEEIQDIVEEVLINNRLIKVAKSYILYRDQHARLRNLS
                     LMIDSEKIMDDYLKQTDWRVKENSNMSFSLQGLNNHLASIISSTYWLNKVYNAPIREA
                     HQNGDFHLHDLQILAAYCAGWDIKDLLLKGFGGVSGKVESRPPKHFRTALGQITNFFY
                     TVQGEVAGAQAFSNFDTYLAPFIRYDKLTYTEVKQAMQEFIYNVNVPTRVGFQTPFTN
                     ITMDLKPSKAMGEENVIIGGELKEERYRDFQPEIDMLNIAFAELMMEGDAKGRVFSFP
                     IPTYNLTKDFQWDSPVANKIFEMTRKYGIPYFSNFINSDMDPDDARSMCCRLRLDNRE
                     LRKRGGGLFGANPLTGSIGVVTINLPRIGYLSQTKEEFFQRLGNLMRLAQESLETKRE
                     IIEKLTEGGLYPYARYYLASIKERFGGYWNNHFGTIGLIGMNEALINFAPIANSIASP
                     AGHQFAEEILEFMRETLGKFQEETNHLYNLEATPAEGTSRRLARLDRDLYPNIKVANN
                     DKIDQAPFYTNSTQLPVNFTNDIFEALELQDSLQTKYTGGTVLHFFLGESLPSASAVR
                     TLVRKVAENYHLPYFTITPTFSICPKHGYIAGEHFYCPKCDEEIGFHPEPALAEMA"
     gene            27623..27781
                     /gene="nrdD"
                     /locus_tag="PKV49_00115"
     CDS             27623..27781
                     /gene="nrdD"
                     /locus_tag="PKV49_00115"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPL22066.1"
                     /translation="MSPLETKRTPCEVYSRVTGYLRPVKQWNDAKQAEFANRQTFIAE
                     GESDSCGC"
     gene            27784..28515
                     /locus_tag="PKV49_00120"
     CDS             27784..28515
                     /locus_tag="PKV49_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPL22067.1"
                     /translation="MIIGGLQPVSLLDYPDKISAIVFTQGCNFHCRYCYNPMLVRPAT
                     VGVTNTTGQPVYSLADLWAFLDQRQDRLEAVVVTGGEPTLHHDLPEFITEIKRRGLLV
                     KLDTNGTASQMVKQLVQDGLIDYLAMDIKAPWNKYDQIMPLPADILSRLTAEAKITAE
                     WLLTDQPLDYEFRTTVVPQLLSAADISLLAEQLIGARSWYLQKARLAGDLLDPSLVGQ
                     RVYTDAEMVELLAIAKAKVPTAQIR"
     gene            28573..28788
                     /locus_tag="PKV49_00125"
     CDS             28573..28788
                     /locus_tag="PKV49_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22068.1"
                     /translation="MDEDLRRVLADQAAAIAEIRENTRLIYRYVYWQRILSWIKWLLL
                     LLSLLAGVIYLPPLLKDYFSLYNSILR"
     gene            28851..29912
                     /gene="prfB"
                     /locus_tag="PKV49_00130"
     CDS             28851..29912
                     /gene="prfB"
                     /locus_tag="PKV49_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPL22069.1"
                     /translation="MGGGFDLAAKQRQLATLQAKAQTPDLWSDPEQAGQLLKRIEVIQ
                     TEINNYQATEQRLADLAALAATGEIDAITADLVQTELQQIIQLIEQLENSRFFDQPHD
                     DLPVILSIHAGTGGVDAQDWALILERMYLRYADRQGWSSQVLDEQAGNEAGIKSVTLR
                     LAGPLAYGFLRGEGGTHRLLRNSPFNAQHLRQTSFAMVEVIPELPTIEAPAITESELQ
                     IDVFRSSGPGGQGVNTTDSAVRLTHLPSGLVVTCQSERSQHQNKVLALAVLQSKLAQQ
                     QAEKLNQQKQTLKGQQIKADWGLQIRSYFLYGSQIIKDHRTAVSTSDVAGVLDGKLEL
                     FLKAYWLWDWQRRQADPSA"
     gene            29931..31415
                     /locus_tag="PKV49_00135"
     CDS             29931..31415
                     /locus_tag="PKV49_00135"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPL22070.1"
                     /translation="MSGGGQFFPASRYWLAFIFGIALVGLWPSHWPVAYFYGGLLGIA
                     LAGLTGKRKQSVLVLALCLAIGFWRADTALVSAVTTASSLSSSGDMIIKGRLVSRPQP
                     RSSGGSQMIIKDINYYSHSGWRTWPGRVLVYGPESWLSGAQAGKSWQFSGRLAAWPAG
                     GVFSYRYYLLNRSARAILQADQRQLVASQPQNWWFYLVNGQERLSQALANSLPEPAAS
                     LAGPLVWGGAGQISAEWRQRLAITGLSHITAVSGFNISLLLVLFIDNFIILGWPRRWA
                     TISASLVIAIYVLVIGAPASAVRAGLFGLLLVWGRYCGRLGRSGHLLLISAAVMLAIN
                     PFYFWGDLGFSLSFAAVFGLLYWTELWQKWLRRIPLLRLPLISENLAVTLAAQMWTWP
                     LIFWQFGQISMIAPLANLLVVWALPWLTVFLLLASPLAWLWPAGGVFIFAPAGWLLKI
                     LLAIIANLANWPAAGFIVEWPSGWRIGIIIIYYGLTVVLLRRRK"
     gene            31486..34113
                     /locus_tag="PKV49_00140"
     CDS             31486..34113
                     /locus_tag="PKV49_00140"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPL22071.1"
                     /translation="MTELAKTYEPQNYEAAIYQRWLDSGLFNPDNLPLPAEAPNYTII
                     LPPPNITDKLHLGHAAMLAIEDLLIRYHRARGYRTLWLPGTDHAAIATQNVVEKLLYQ
                     TEGKTRHDYGREEFLRRVWEFVGPTRETILHQIRRMGASLDWSRMTFTMDEPRQKAVR
                     VMFKQMYDEGAIYRGERVVNWCPRCQSTLSDDEVDYKEQPAELYTFKYSADFPIAIAT
                     TRPETKLGDTAVAVNPQDKRYQQYIGQTYQVDFCGQPLTIKIIADRSVDMAFGTGAVG
                     VTPAHSMTDWQFKEKHDLAVVKVITPDGLMADNLGRFSGLPVAEARQLVVAELGQRQL
                     LIQQTTITNNLSICYRCGTAIEPLPSKQWFIGVDRPLDRLGGRSLKQAAMAAVEQGQT
                     KFVPERFQKRYLDWMANLHDWCISRQIWFGHQIPVWYRGEEIMVSDTAPHGGDWQQDP
                     DTLDTWFSSGMWTFSTLGWPDNFVDGQKIGDLARFHPTQVLETGYDILTLWVSRMMMM
                     TLFALGEVPFTDVYMHGLVLDEHGKKMSKSRGNGLDPLDMIELYGTDAVRLSLLIGIT
                     PGNDCRLGTAKIASYRNFVNKLWNIGRYILSALPDKTVFVHNNIDLTQTTWADRWILS
                     RFDKLVKTVGQRISRYEFSLAGEALRDFTWNDLADWYLEISKMEPTSQKANILGYIFK
                     NLLILWQPFIPFVTEVLWQQLGIDQPLLIAQWPEQLSGDWADAESFTIVQQLITEIRN
                     LRSHQQIAPNQNLSALIIAGQQAEALNSQIDLLKKLRTGLEQVEIVATGSAPADSLAG
                     VCGQISFYLFGQIDQTGAQAKIAKKIVELTTAIEQLSIRLANPEFIAKAPKKIILAEQ
                     NKLADWQAELTALNQQITK"
     gene            34115..34879
                     /locus_tag="PKV49_00145"
     CDS             34115..34879
                     /locus_tag="PKV49_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPL22072.1"
                     /translation="MWFLNLLLSLLAIVITWLLASWSASFSKRWANRHKDNKINPTMH
                     LIVQRAIQTVIYILGLLTIGQIWHINIGPLWAGLGVAGVAIALGLQETLSNLFAAFFV
                     IIDKTIRVGAYLKLDDGTVAKVEDISWRSTRLKATSGERIIMPNRLFANQKVIGRSQT
                     DKNWMVTIEAIAGPGSQLTEVEQGALAAGQIVAKKYPAKTATVKFYFDELTAAGLKFK
                     LVISIDSAANETAARHELLKNLIIELAKRSCPLVGR"
     gene            34884..36083
                     /gene="tyrS"
                     /locus_tag="PKV49_00150"
     CDS             34884..36083
                     /gene="tyrS"
                     /locus_tag="PKV49_00150"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPL22073.1"
                     /translation="MIKTDAKLIDELLERGVNEVIIKDQLRQKLLSGQKLRIKLGTDP
                     TSPNLHLGRSIPLLKLRDLQQLGHQIVLIVGDFTGVIGDTSDKDSERPMLSPEKIQAN
                     MAQYLKQAGQLIDLDSCEFHYNSEWLGQLTYNQIGEQADQFSVAEFIARDNIRRRLEA
                     GSRVSVREMLYPLMQGYDSVMVKADVELGGTDQRFNLLAGRKLQEHFGQPPQNIIMTE
                     LAEGTDGRKMSSSWGNTINFTDEPQTMYAKVMGISDDLIIKYFILMTRCPLSQITEYE
                     QAIQGGANPRDAKMALAKEIVNIYHGQVAAEQAEAYFIKVVQQKLLPEDIATIALTKS
                     DWPIIDLLLDIKAATSRGEARRLIEQGGVKLAEQTITDSQLLVKLSAKPILLQKGKKQ
                     YWYLVAE"
     gene            36085..37785
                     /gene="argS"
                     /locus_tag="PKV49_00155"
     CDS             36085..37785
                     /gene="argS"
                     /locus_tag="PKV49_00155"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPL22074.1"
                     /translation="MNGYYYQDITSKLADLINQQLPEPLVQADDIVIPPQADMGDLSW
                     PCFQAAKQLGIAPGVLAQDLVAKLATGNNPMISGWQAAGPYLNINLSPQLLAEKVLTE
                     INQEKENYGRSAEGQSKRIMIEYSNANTHKEYHIGHLRNLFFGDSVQRILNFVGYQAI
                     PVSYLNDFGVHVAKVLWKLSQQPAEQIIPDNRGYYLGQLYAQASQELPNDPVGQGLVG
                     AIMKRLEAKQQPEYGRWQETRQWSVDQFNDIYRQLDIHFDQTFFESDYLDDGRQLVEK
                     MLAAGQLEKSQGAVLANLENEKLGVLLFLRSDGTTLYPVADLPLAQDKFKQYQLAESW
                     YVIDERQSLYFQQLAAVLRRLGINQPIRHLSYSFVKLPTGMMASRQGNIITYEQLKQD
                     LLNKELAEIKQRHADWSEEQCQQVAWQVVVGALKFEMVKVGPDKPITFDLNQALSFDG
                     YTAAYIQYAAVRLAAIQRQAAWPESLVVPIAPIEEKAILLTAAQFNQVISQAAHQADP
                     SILAKYLFNLARQFNDYYQRVRIIGSQEQAFRLHLVAAIRQVLDNGLRLLGIQAPDLM
                     "
     gene            37790..38815
                     /locus_tag="PKV49_00160"
     CDS             37790..38815
                     /locus_tag="PKV49_00160"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPL22075.1"
                     /translation="MLPNELLEQLRSELAIVTDESGLGVVWQKYLGKQGLIKKQVKAL
                     SALTIEEKRQQGPAWQAAQQTAQQLFDDHSAELAAVSRQATCQAQAQTIGLKPVKLGH
                     LHPITQTIRQLNDLFIKMGYSVMEDREIETDEFCFQRLNVPMDHPARDMQDTFYLQEP
                     NILLRTQTSSVEARLLANYQPPFKVVCPGRVYRNETVNKSNHFIFHHYQGVVVMNKTS
                     LADLFGTFTALFQSVYGPEVKIRFRNKYYPEVEPGVGPDMQCFNCQGAGCPICKGVGW
                     IEMGGAGLIHPKVLTMAGLDPEQWQGFAFGLGLDRWAMARHNITDVRTLLGGNLGYKP
                     YSHENTL"
     gene            38799..40760
                     /locus_tag="PKV49_00165"
     CDS             38799..40760
                     /locus_tag="PKV49_00165"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPL22076.1"
                     /translation="MKILYSQIKQLVPGLVATPAEVGEALTMTGLMQESLTAVNYNGQ
                     ADTLIGLEVRQNRADCFSVLGIAREVAAYFNLALKLPDLKPWSPTNFILPIRIDTDQV
                     RRVRAVKLSGAVAKPSPQWLVDYLSLYEIKTVNLAVDLSNYVMILTGYASHLIDYDRL
                     DGDVVWSVNDRAVKVTTLDGSQIDLTGGELLISDRREIIALAGIVGTDYAAIKPETTN
                     ILLEMAAYNPSLVRQNSRRLHIVTEASNKLEKDLSLANLDYAFDLLVNLLVDQIGVQV
                     ASQLFEYYPQPTQPLVIKFDPQLAAQMGGVEISSQQSQQILQQLGFVIDNKNNPWLVT
                     VPDWRTDLEMAQDLVEEVLRLYRFDKIPAILPALTVTPEITLPLTKAKEQWRDWLVAQ
                     GWDEVLSWTMVTRANNQEANYLPWSIASTQNAINEDYPDLRQTILASLIQQAREYLKK
                     QVPDLQIFEIGKVFGQVGDDYQEAEHLGWLLAGQDTVVEQLRQTVAGFLGWAGAKQIT
                     WSQLDNIPPAGNPYAAWQIQLAGQSLGILCQLIDDYNGQTMVAAEIDANQLINHLINV
                     GNFPATVELTQKLISLDANLELDNRQALDAELADITAKVGYEGLWSLSVVDEFALPNG
                     RYRYTVRAVYKELDDQSAKKLHLQVFGLN"
     gene            40774..41202
                     /locus_tag="PKV49_00170"
     CDS             40774..41202
                     /locus_tag="PKV49_00170"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPL22077.1"
                     /translation="MGLLRLARRIIDWAFKQFYSPQFVRYFIVGCSAVLLDMATLYWL
                     KTIIGLTAVWSIVINQLAMCSYVFLLNKYWVFASSGAFARQAVRYFSLAAANYLIAIF
                     WMWLFNEQFGFNYLLVRLVNIALSTVWNFLLYRFFVYVKK"
     gene            41307..41642
                     /locus_tag="PKV49_00175"
     CDS             41307..41642
                     /locus_tag="PKV49_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPL22078.1"
                     /translation="MELHDKEFLEYVVKAIVGNPEDVSVDRTVDEMGVLLTLKINPAD
                     MGYVIGRRGQTAQAIRTLLKIVGAKNSARVNLKIYDPNGGQRPHREFHQEKPVEEKID
                     TSSVEDLAI"
     gene            41710..42369
                     /gene="trmD"
                     /locus_tag="PKV49_00180"
     CDS             41710..42369
                     /gene="trmD"
                     /locus_tag="PKV49_00180"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPL22079.1"
                     /translation="MLFQIITVLPEAFGYLEQSIIGRGQQQGHFQVKIHDLRNWSLNQ
                     QRRVDDTPYGGGPGMVMQVEPIAQVLKAIKTKHKHKKIVLLSAKGKTWTQARARQYSK
                     LDEVVLICGRYEGVDERVNNLIDEEISIGNYVLTGGELGAMIILDSIARLLPGVLGNA
                     DSLLQESHSRVGQLEYPQYTKPEVVNLAGIDYAVPEILLSGNHQEIAKWRQAQMKRIK
                     K"
     gene            complement(42415..43449)
                     /locus_tag="PKV49_00185"
     CDS             complement(42415..43449)
                     /locus_tag="PKV49_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22080.1"
                     /translation="MQDFKQWLPEILNWYKDCGFSSNFTAAHGRGHIIRDVLNTLIML
                     PGTPMHPDDALAAAIAGLTHDLAVGTYNCAEKNNIGHADIGGYLLRLCLRHIGFTAKN
                     PYAAGMAVFSAAAHTFYTEDRTVLVGGQEYKLAPYPTLAPNGEVLVASDVTKAIDQAD
                     CVSGFFVGRYLINIWDVINYVFINQLGVIKYVDGQFPTGSALRHFTALANNQLTHSQL
                     NRYHYGDVRRVLTTYAPWLMDIINEIYNRFITGNTGGESYQLAVEKWLVEKVTYFDGL
                     NEAKVVMYNLMNLDEPLKTAWLKGLNLAREHTRAQIRFMRQELGDERIAQLPKNLPIL
                     GSGLHDMLAT"
     gene            complement(43717..43800)
                     /locus_tag="PKV49_00190"
     tRNA            complement(43717..43800)
                     /locus_tag="PKV49_00190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43764..43766),aa:Leu,seq:caa)
     gene            43887..44645
                     /locus_tag="PKV49_00195"
     CDS             43887..44645
                     /locus_tag="PKV49_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPL22081.1"
                     /translation="MTKIIAIVNQKGGVGKTTTAVNLAAYLAQAKRRVLLIDIDPQAN
                     ATSGSGLISASQATGLYEALVGQQTMADIIIDSPAGYRVAPATINLAGAGVELVNLPE
                     REFRLAKVVDEVRTDFDYIIIDGPPSLGLLTINSLVAASHVLIPVQAEYYALEGLKQL
                     LDTINLIQTHIKPNLGILGAVITMYDQRNRLARAVVKELEQFFPNRVFKSIIPRNVRL
                     AEAPSYGQTIVEYDRHSSGSQAYQALAKEILALE"
     gene            44661..45506
                     /locus_tag="PKV49_00200"
     CDS             44661..45506
                     /locus_tag="PKV49_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPL22082.1"
                     /translation="MPAGLGKGLGSLLAANPLVEPMDSERIVNLAPDLIQVNRQQPRH
                     DFNQESLQELADSIKECGILQPLIVAKTTAGYELIAGERRLRAAKLIGLKTVPAMVRS
                     VDEQQKLALALVENIQRENLSPLEEATAYRRLLDEFQLTQDELASRVGKPRSTITNLL
                     RLLNLPVSAQQALRSGQINLGQAKVLAGVDPAKQDQALREMTAKPITVADSVKVARIF
                     NSRTQSKTTPTAKDVYNDKGKEFLLREFFQTKVKITRQARGGQIIISFDSDDQLSALM
                     NKIRR"
     gene            complement(45556..46077)
                     /locus_tag="PKV49_00205"
     CDS             complement(45556..46077)
                     /locus_tag="PKV49_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPL22083.1"
                     /translation="MDLLTGLTIVSSLILLSLATGAWSAAPWVPTRQRDWAAINQLLD
                     LQPGQIFYEIGCGHGHLLAYLARKNPDSRLIGLEISPLLALAAAWKTRRQANVTIRWA
                     NLWHYRLDDADAVYVFLLPKIYPQLIKKFKSQLSPATKIVIGDWPLAGYQPQRKLRPV
                     GSVPYYLYRASDL"
     gene            complement(46079..46948)
                     /locus_tag="PKV49_00210"
     CDS             complement(46079..46948)
                     /locus_tag="PKV49_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPL22084.1"
                     /translation="MIIPLPVWLKWLFFWLLALTIALTLNYQNRHLTIKQYNINDQRW
                     PAELTGWRIVQLSDLHNQTISGPGNRQIIAAVEKLAPDLIVLTGDTFDSRQTNIPSNL
                     FLINRLKNLAPVYLILGNHDFYNNDYIELAERATAAGAIVVRNQALTIQSQNQTITLV
                     GLDDPTGLGGPLPASVVAKITSQKLGQIINQNKVNQPIIVLAHRPELWPEYLSIEPIA
                     ILAGHTHGGQIRLPLLGAIYVPNQKLWPDYDYGNLPVGNSRLIINRGLGNSSFNQRIN
                     AYPEIVAINFNHH"
     gene            46997..48451
                     /locus_tag="PKV49_00215"
     CDS             46997..48451
                     /locus_tag="PKV49_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22085.1"
                     /translation="MLIKLNRPVRWWFKQTKVARVLLTASLVGWLVLLYLASRPFGVA
                     VYSLSSQSGNYFIHKFGPTERWSTSSPDLIMTGQPGYFFLRPTRPFNQAEITVTWQDR
                     QPDTYLEAGILMDRANWQYQSQPLNHPGLNKLDWPLVTAGHHRLWQKTPVYQTWSEFI
                     ERLPNFSQLAVYNWSPSSTKSINLTGYRSHQVWQQLPGQWRGLQQIVTYLADDEQLAW
                     RITVTSDSLQELTADERLVEVNITNSVNQRLWSEQRRLDDNQLEWLITAGPWSAGAYR
                     LEIKASRQLLLKLATQQTVFGWQGQVWPVASADYHWRFWTDAPAIIAQTIQPASRQII
                     LVNQRPLNLADTYQQYSIETLATSSEISLSGGDVQLAGSGIFSLQPTIFNPQWRRAGR
                     LSVDQLAGFDFIAADYQPPVCRQHLCTATAKLSLAGADWQSGDGYQIMLAADNSHLST
                     VSPLVIQEISVKLRGASLWQFLRSLPRRLVNKLQ"
     gene            48455..48649
                     /locus_tag="PKV49_00220"
     CDS             48455..48649
                     /locus_tag="PKV49_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22086.1"
                     /translation="MSIRRQIKIKQLNDDLYLWLTVTIAVLIFSEAVWPGLVSAYLNL
                     SFWLLFWSVSAILKVVFVNK"
     gene            48668..49120
                     /locus_tag="PKV49_00225"
     CDS             48668..49120
                     /locus_tag="PKV49_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HPL22087.1"
                     /translation="MIQDVIFKKLTVYEDARGWLAEIYRNDEDHYQPAMGYISVTRPG
                     VARGPHEHKYQADCFVFVGPGDFELYLWDRRENSSTNGQHIKEVVGQSNPTMVIVPAG
                     VVHGYKAIGNQDAWSVNLPDKLYRGVDKTEEVDEIRWEQNPDSPYKII"
     gene            49137..50114
                     /gene="rfbB"
                     /locus_tag="PKV49_00230"
     CDS             49137..50114
                     /gene="rfbB"
                     /locus_tag="PKV49_00230"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPL22088.1"
                     /translation="MRLLVTGGAGFIGSNFIHYWLQKHPEDQLINLDLLTYAGNLANL
                     EDIANQANYRFVQGDIADYAVVDSLMGQVDCVINFAAETHVDRSIGDSLSFVKTNIQG
                     TANLLEAARRHGGVRFHQISTDEVYGDLTRHDPKFDETSPYRPSSPYSASKAAADHLV
                     RAYFRTYKLPVTISNCSNNYGPYQFPEKVIPLFVTNLIDDLKVPLYGNGGNIRDWIHV
                     DDHNAGIEAILQRGQIGETYCLGGDHEITNLELTKQIIQLLGKDDSYIEPVTDRLGHD
                     WRYAIDFSKATRDLGWRPAIPFSTGLKQTVQWYQDHPEWWRPLKEQVKQ"
     gene            50118..50981
                     /locus_tag="PKV49_00235"
     CDS             50118..50981
                     /locus_tag="PKV49_00235"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPL22089.1"
                     /translation="MKVLILGGLGNLGSQLVVTGQQLGYEIIGWDQEDIDLTDWPAVE
                     AKLAGQRFDVVINTVAYNAVDACESQPGQDLAYILNGDLVRQLAGWCQTNGAIMVQYS
                     SDYVFAGDQLAGYTETDQPNPINEYGRSKLAGEQALIKQGEQGLQYYLIRTSKLFGPR
                     GTSPATKSSFFDIMLKLAATEPELKGVDAEESCFTYTPDLAQATWQLLADKFPQGIYH
                     IVNSQPATWYQALRTLQQLAGLTVPIRPITSADLPRPAARPQHSVLLMTKLPPLRSYQ
                     QALQEYLNKSK"
     gene            50986..51711
                     /locus_tag="PKV49_00240"
     CDS             50986..51711
                     /locus_tag="PKV49_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPL22090.1"
                     /translation="MRGIILSGGSATRLRPCTKVTSKQLLPIYNRPMIYYPLNTLVKA
                     GIKEILIIVSPERAGDYLNLLGSGRQFGIKLTYEVQDKPRGLAEAFIIGENFIDDGDV
                     TMILGDNIFEDDLTDDIRNFKSGAKIFAKKVPDPERFGVVEIDDKMQALSIVEKPLQP
                     KSDLAITGLYIYDHRVIEVAKQVQPSARGELEIVDLHNWYLERNELEVAMINGEWIDA
                     GTFDSLLSAQILARDRLQNKLII"
     gene            51730..52785
                     /gene="pyk"
                     /locus_tag="PKV49_00245"
     CDS             51730..52785
                     /gene="pyk"
                     /locus_tag="PKV49_00245"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPL22091.1"
                     /translation="MHTRIIATVGPQSFSYPVLSKLVTSGVDIIRLNFSHAKIEQLND
                     LKNNLQRIKEETDCQTKIMQDLQGPRIRIGRLDQEIELVDGETYVFTGGQASLANQEI
                     PIDDPDLYQDLRIGDPIFLANGELELKIVDIKDKMIYARVERGGLLMSHKGINLPNTN
                     LTRGGLTKKDIADVEAAFEYNVDYIALSFVQSADDLRRLRKIIGNRPIGLIAKIERAI
                     ALEVIDEIIKESDGIMVARGDLGIELPMEDLPIIQKNLVRHAHWHGKPAIIATQMMTS
                     MIEHPRPTRAEVSDIANAVLDGADALMLSDETAVGLYPNQAVAAMKKIICRTESYLNN
                     RNYFDTETAAFYCHYVN"
     gene            52791..53819
                     /locus_tag="PKV49_00250"
     CDS             52791..53819
                     /locus_tag="PKV49_00250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL22092.1"
                     /translation="MAQSSRVYVGFLGYGQSTAKYLPDFLASLRQQTFTDLAIIDGDN
                     TPPDQVNPNELAIKDFPEVRYWPAGANLGFAKGYNRLFNLAIREGAEYFLVINPDVVL
                     ESTAIEQLVKYLDDNQAVAAVMPKIRRWDFVNHNLTMQIDSLGVGLTKQGAFIDIGQG
                     QLDDNHLTGPQPIFGFTGAGVLIRLSALSSVAFDNGQSIEYFDELMFMYKEDCDLSYR
                     LRLAGWQIMLLPEAIIYHDRTAAKVGQSVWSQLRNRRRESKLIRQWSYLHQLMIFCKY
                     WYLPKTLSSRWQELFYQKKRLVAAILFEPFLLSGWRKLWQQRHLVAARRRALVIKIKP
                     LSLEDWRQ"
     gene            53824..54687
                     /locus_tag="PKV49_00255"
     CDS             53824..54687
                     /locus_tag="PKV49_00255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL22093.1"
                     /translation="MLDLAIVTVPFKVKDKLRDNLRQLFASQGDLNFAVWAVDNNSGD
                     GTVEMLQQEFPQVHLIANPDNRGFAKANNQALRVIRARYVLLLNPDMLVEPDTLVKML
                     AWADANPQATVMGCHLVTETGETLLHVRRFPTLWDQLAIVLKLPHFLPHILDKYLRTD
                     FDYSQAAPVDSIRGGFFWIRADANGQVPLLDERYFIWFEEVDYCRQTKLSGGQVWYTP
                     AANCLDYVGQSFKQVKRGRTQTYFRQSMIKYFAKWEPRWQSYLLRLAWPIGASLAIIG
                     EKLNWQGRAKT"
     gene            54697..55776
                     /locus_tag="PKV49_00260"
     CDS             54697..55776
                     /locus_tag="PKV49_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL22094.1"
                     /translation="MTTYKIATILINYQDYANKYLADCWASLEKLNLATDDWQIDHTI
                     FIVDNQSDDKTYQTLQTMAPTARLIRNATNDGFAKGNNDAIAIAMAEGCDFVWVLNMD
                     TIVEPDSLIELVKPMLVNQKLGAAQSLMRLWPDQDLINSSGNATHFLGYGYCLDYRKP
                     VAELNLAAIDGQAIAYPSGAAVLFRLSALRQVGLFDETFWMYNEDQDLGWRLWLAGWD
                     CRLAAHSQIYHKYQFSRSSSKYYWLDRNRLLSGWKNYHWLTIIVLLPALLSSDLILAI
                     MSWRAGRLKHKLNAWWYFINPLHWPMLLKSRWQSQRLRRRPDRQIIKIFSGRILFQEV
                     DSQTVRWGNWFLNFYWQVIKLIIIW"
     gene            55778..56596
                     /locus_tag="PKV49_00265"
     CDS             55778..56596
                     /locus_tag="PKV49_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL22095.1"
                     /translation="MDISVSIVNYYSQKVIYRCLESLRQTNWHGLEHEIIIVDNSHET
                     DLAAKIAKEWPEVRYIANSNTGYGAGNNLALRQATGKYFLVMNPDTAVAPDTIKQLYD
                     FMEAHPQVGMVGPRQRNLDGSRQASCYRWHTLATPFCRRWPWLSRYRWVRRELRRFLL
                     EDVDLERTMPVDWLLGSFLLIRAAAMRQVGYFDERYKLYFEDTDLSRQMWYHGWQVVY
                     YPGTEVLHNHQRASAQAAWYKVLNNRVGRQHTLSWFKYLLKWGLIAKSNIPRNQ"
     gene            56604..57473
                     /locus_tag="PKV49_00270"
     CDS             56604..57473
                     /locus_tag="PKV49_00270"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPL22096.1"
                     /translation="MRKITKAILPVAGFGTRFLPATKAQPKEMLPVVDKPAIQYLVED
                     AVAAGIEEIIFVTGRGKRAIEDHFDYSFELDKTLVEKHKLDLVKQVRRISEMAKFSYV
                     RQPLPLGDGHALACAAHLVYDEPVLVMFGDTLYDADEGPASQVLKTFDKYGDPVIGLS
                     AVDRQEVSKFGVIDGLALADNVYEIKQFIEKPSPQEAPSNLVAVGIYVITPDVLHTLT
                     MMKPRPNGELRLADAFVQMLAADRPLYGQELIGTWLDTGDKLSMIKANIQLGLKNKEI
                     GADLRRFIEGILR"
     gene            complement(57474..58340)
                     /locus_tag="PKV49_00275"
     CDS             complement(57474..58340)
                     /locus_tag="PKV49_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPL22097.1"
                     /translation="MSLLQRQWLSRLFILLFLIITPLVLAYATGYHINWRNLKLEKTG
                     LLEIKTIPSGAQVNIEELRAWFSKPKNLNTPFKLKNLRPSNYTVNLSLDGYHSWRKIL
                     TIKPAETTFAKDVRLFKQAEPQWQADLPASSTVDTASLLGNNQLNLNNKNLTTDTIND
                     LIPNSSLIDNWLDTRSIHLACPLPDKKIAYASQFELWLADPDHKTKVLLGRFSQTINQ
                     LICLPDTSNYLIYGHDSSWQLIELDDRQGYNIWQLAELDELGAAAVNKKVDQLYFYGR
                     IGTKSGLFSLAL"
     gene            complement(58346..59605)
                     /gene="murA"
                     /locus_tag="PKV49_00280"
     CDS             complement(58346..59605)
                     /gene="murA"
                     /locus_tag="PKV49_00280"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPL22098.1"
                     /translation="MSKFIINGRQPLGGQIKVGGAKNLALKIIPASLLSDQPIIIDNL
                     PDIEDIKRSLEIIKSLGGLVDRQTDQCRIQTKNLATTIIPAELANKLRASIMFVGPIL
                     ARMGQVSFPHPGGCVIGAGTRPIDLFLEGFRSFGAQIEFADGYYHLAAKQLSGANYFF
                     TTISVTGTEALMMTAVLAAGQTVLENCAMEPEIVALADYLNSQGADISGAGTSTIIVN
                     GVQSIGGGAFTVMPDRIETGTFALLAAASRCELLITDCFPDHISVLLNIFRKQGVNFE
                     RGDNWLKMLPSKNITAYSIKTHEYPGFPTDLQSPYTVLMTQAQGSSIVHETIYDRRLL
                     FTDMLTQMGANIIMCDPHRVVINGPTPLHGRKLTSPDLRAGMAMVMAALIAEGKTEID
                     NIYQIERGYQQIAERLRAIGADIERVE"
     gene            59702..60973
                     /locus_tag="PKV49_00285"
     CDS             59702..60973
                     /locus_tag="PKV49_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPL22099.1"
                     /translation="MFQRRSTKCWGRFLGWLFLVAIIASGGFLFGLFYGQRAELARRL
                     SDSQAVFLGRLTGIYQQDQQGRLISDIDFSLFWQTWDLIKANYVDQDKLTDKQMFYGA
                     LSGLVSSVDDPYTVFMNPEESKLFNEDLTGTFSGIGAEVGLRNDVITIIAPLAGTPAE
                     RAGLRSGDKVYAIDKQSTVGLTVDEAVKKIRGEKGTTVILTIARNGDKLRDISITRDT
                     IVVKSVATDYDQTSGLYTITVSNFNADTEPLFQAAVQDIEHQKPRGLILDLRNNPGGY
                     LDAAVAIASFWISDGVIVSEQFSNQSAIQSQHARGLAPLADLPTVVLINQGSASASEI
                     VAGALMDHQQATIIGKTTYGKGSVQVVRDLPDGSMIKITTAKWLTPNGTSINDKGIKP
                     NIEVELTEDDFNKNIDPQMVKARQFLLQEDK"
     gene            complement(61034..61306)
                     /gene="rpmA"
                     /locus_tag="PKV49_00290"
     CDS             complement(61034..61306)
                     /gene="rpmA"
                     /locus_tag="PKV49_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPL22100.1"
                     /translation="MAHKKAGGSTSLGRDSQSKRLGVKLQDGQIAKAGSIIVRQRGTK
                     YRAGNNVAVGNDHTLFATAAGPIKFRTKKLRRFDSHLKNTKIVEVG"
     gene            61442..63076
                     /locus_tag="PKV49_00295"
     CDS             61442..63076
                     /locus_tag="PKV49_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22101.1"
                     /translation="MTKPLITSKFFNRWLISLLIIFGFLLLLRNSFSYLDPDFGWHYR
                     VGQEIWQSRQLPRIEHHIFPVAGQNWVDHEWLSNLLLFLITDLGGYFLLSLLFAGLII
                     LTWWLLARSTIRHWLDGHGDCLIFWLLLIIGFVACLSNFGIRIQEITVLFLLILNLWL
                     AKVTRQRLWRPWEIIALPVFFWLWACLHGGFLIGLAVIITWTFFRGILYYLHRHQRSA
                     VSPTNLLPLKTIKQSTIVAGLSLVATFLTPYGWRLYSFLADYQSNRAYLKFIQEWQPM
                     TEAIWSWRMLYSLFFGMALVLLYFHRRNQAKTDRQIIQWLWQQDWWSLLLSLIMFIAA
                     WRSLRHFPLFFVVSLPWLAGFYYHSWQDCHFQPSRWPAYLKWFISAVAILSIVIIPIS
                     THWTNQPTTDYCAKYPCSALDYLRQSPYHNWPTIVDYGWGGYFYWQWPEKSIFIDGRL
                     PQYPWRDRTLIEHYSRFFIESDIKDALNDYDLSVIVLAQPAPVKFNWLETWLLGRGQQ
                     TTDYETSQEVFRRAMLKLPNWRLVYSDMVATVYVRQ"
     gene            63063..63815
                     /locus_tag="PKV49_00300"
     CDS             63063..63815
                     /locus_tag="PKV49_00300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22102.1"
                     /translation="MSGNKRITLDLCLPVYNEAAILASNVQRIVDFISSNQLAVDCRL
                     IILVNGSTDQSLQIAQELSQRWPNLVSYKNYEVASRGSALLAYADISPADWWGFMDID
                     LAVDLASLQQFFKLMVDQSADVLLASRLMPQSSYKHKLTRKIISVVYNQLARFALKTS
                     VKDHQCGFKFISQSWWKTLRPYLQDSRWFLDTEIIAWTTKLGGRIVEIPVDWVNNRYH
                     IRPTKIKLIRDISNFLINLYCLRKRLRAHRSS"
     gene            63915..63987
                     /locus_tag="PKV49_00305"
     tRNA            63915..63987
                     /locus_tag="PKV49_00305"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63948..63950,aa:Lys,seq:ttt)
     gene            64011..64086
                     /locus_tag="PKV49_00310"
     tRNA            64011..64086
                     /locus_tag="PKV49_00310"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64044..64046,aa:Thr,seq:tgt)
     gene            complement(64140..64322)
                     /locus_tag="PKV49_00315"
     CDS             complement(64140..64322)
                     /locus_tag="PKV49_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HPL22103.1"
                     /translation="MAKQCDICQRSATKGASRSHSNIKTIRRQGINLQNCKVDGGTIK
                     VCTSCLKTAKKWEGKE"
     gene            64441..65037
                     /locus_tag="PKV49_00320"
     CDS             64441..65037
                     /locus_tag="PKV49_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPL22104.1"
                     /translation="MISLFQIIIFLLSAILHEYGHAWMAMRLGDDTAERAGRLTLNPL
                     AHLDLFGSLLLPGILILTGSPVVFGYAKPVPINLHNLRGGLAGQAKVALAGPAANLLL
                     ALAAAGSIRYLAGGLISEWGQLLAVVVLINLVLMVFNLLPIPPLDGSKILVTILPKSW
                     RYSYLATERWGFFILLAIMLTWPELLYYPVSWLLSILL"
     gene            65156..65578
                     /gene="rpsL"
                     /locus_tag="PKV49_00325"
     CDS             65156..65578
                     /gene="rpsL"
                     /locus_tag="PKV49_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPL22105.1"
                     /translation="MPTINQLVRKPRTTISQKSKSPAMQVTMDSLHRKRNIIQQGSPF
                     KRGVCLKVTTTTPKKPNSALRKIARVRLSNGMEVTAYIPGVGHNLQEHSIVLIKGGRT
                     KDLPGVRYKVIRGVYDTQGVDGRRQSRSLYGAKAPKAK"
     gene            65593..66063
                     /gene="rpsG"
                     /locus_tag="PKV49_00330"
     CDS             65593..66063
                     /gene="rpsG"
                     /locus_tag="PKV49_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPL22106.1"
                     /translation="MRGKQAPKREIVGDPKYNDLEIAKLINYLMKNGKKTTAERIVYD
                     CFDILKEETKQEPRHIFNKGLKMICPLVEVRSRRVGGSNYQVPHQVRGDRRFTLGCRW
                     LLTAARARKGRSMAEKLAAEILDSIKGESASVKKKLDVHRMAEANKAFAHFARH"
     gene            66140..68215
                     /gene="fusA"
                     /locus_tag="PKV49_00335"
     CDS             66140..68215
                     /gene="fusA"
                     /locus_tag="PKV49_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPL22107.1"
                     /translation="MREFKLENTRNIGIMAHIDAGKTTFTERVLFYTGKKHKIGETHD
                     GQADMDWMEQEKERGITITSAATTCFWKNNRINIIDTPGHVDFTVEVERSLRVLDGAV
                     AVFDGAAGVEPQSETVWRQADKYHVPRLCFVNKMDKMGADFIMSLNSIRDRLNSKAVA
                     IQLPIGAEDNLVGIIDLLLEKAYEFHGQNGEQITEIEIPANLKDKVAKYRHEMIEKIV
                     ELDESLTEKYLNGDEVNVDDLKKALRKGVVNNEIYPVLCGAALKNIGIQQVLDAVNEY
                     LPSPLDVPEIEAHEVGDDSKKIIIKPDDNGPFVALAFKIATDPFVGRLCFVRVYSGVL
                     RSGSYVTNSANGRKERIGRLVRMHANHREEINEIYSGDIAAVIGLKETITGNTLCDEG
                     QNICLESISFPEPVIKIAVEPKTKVDQEKMGIALGRLAEEDPTFLVETDGETNQTLIS
                     GMGELHLDIIVDRMRREFGVEANVGQPQVSYRETIRQDAEAEGKYIKQSGGRGQYGHC
                     WLRIGPQAEGQGSEFVDEVKGGVIPREYIPAIEKGFREALDKGVLAGYPIVDVRCAVY
                     DGSYHEVDSSEAAFKMAAVMAFKDACRKARPVLLEPIMKVEVTTPEEYMGNVIGDLNA
                     KRGQIDHMSDRGKLKIIDAKVPLAEMFGYATQLRSMSQGRANYSMEFLHYNEVPRNVA
                     EQIIGKSGK"
     gene            68250..68564
                     /locus_tag="PKV49_00340"
     CDS             68250..68564
                     /locus_tag="PKV49_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22108.1"
                     /translation="MIKNIDQILRLAQLTGDRLIVADQQNPERSLAVLSLSDYEELLS
                     LAGLTKKQGFAKVDNNVASQEAGNVAVNSSFVQKLGQSQGKKSPWSIPASRQAAQKNI
                     DD"
     gene            68635..69822
                     /gene="tuf"
                     /locus_tag="PKV49_00345"
     CDS             68635..69822
                     /gene="tuf"
                     /locus_tag="PKV49_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPL22109.1"
                     /translation="MTDKYERSKPHVNVGTIGHVDHGKTTLTAAMLTVLTAAGRKTSQ
                     KSVDQIDSAPEEKARGITIATTHVEYESENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPYIVVFLNKCDMVEDKELIDLVEEEVRDLLNK
                     YSFPGDTTPIIRGSALQALQNPTGEYAKSIMELIDTLDTYIPEPQRQVDKDFLMPIED
                     IFSIEGRGTVVTGRIERGIVKVSDEVELVGLGDTRKIVVTGVEMFNKSLDQGMAGDNA
                     GILLRGIKKDEVERGQVLAKTGSITPHTEFEAEIYVLTKEEGGRHKPFFKGYKPQFYI
                     RTTDVTGEIELPEGTEMVMPGDTVTLKVKLITPIALEDKMRFAIREGGRTVGCGVISK
                     IIK"
     gene            69899..70234
                     /gene="rpsJ"
                     /locus_tag="PKV49_00350"
     CDS             69899..70234
                     /gene="rpsJ"
                     /locus_tag="PKV49_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPL22110.1"
                     /translation="MPDKKAAPADYKQRIRIKIRAFDHKIIDQSTKTIIDTVQRTGAK
                     IYGPIPLPTEKKKYTVNSSTFVHKNARDQYEMRIHKRLLDIIDPATKTVESLMNLSLP
                     AGVDLEIKM"
     gene            70459..71064
                     /gene="rplC"
                     /locus_tag="PKV49_00355"
                     /pseudo
     CDS             70459..71064
                     /gene="rplC"
                     /locus_tag="PKV49_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
     gene            71349..72017
                     /gene="rplD"
                     /locus_tag="PKV49_00360"
     CDS             71349..72017
                     /gene="rplD"
                     /locus_tag="PKV49_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPL22111.1"
                     /translation="MIKYQVYNQQAEVVGDQELSQEVFGVAINPALVHQAMVAQLANT
                     RSVIADTKDRSEVRGGGKKPWKQKGTGRARAGSNRSPIWIGGGVTFGPTNDRNFTKKI
                     NKKMLSKAICMVFSDRVASHDLIIVDDLTVKDGKTKELKAIIEKLLAADQQGSQVAKP
                     RTLILTGSSIDSVKLAGRNLPRVQILNDQNINVVSLLRANKFIISQQAIASLQQRYGT
                     VAAK"
     gene            72039..72398
                     /gene="rplW"
                     /locus_tag="PKV49_00365"
     CDS             72039..72398
                     /gene="rplW"
                     /locus_tag="PKV49_00365"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPL22112.1"
                     /translation="MEKQNNSTAVVDEKIAQADRQTIRRGQLAGVLIKPVVTEKATAV
                     NHLNQYVFMVANKANKIDIAKAVLVFYGIEPVAVNIINVAGKKVARGKIKGQRNDWKK
                     AIVTLPAGKTIQIYEGV"
     gene            72423..73262
                     /gene="rplB"
                     /locus_tag="PKV49_00370"
     CDS             72423..73262
                     /gene="rplB"
                     /locus_tag="PKV49_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPL22113.1"
                     /translation="MPIRKVKPTTSGLRGSSFDNFSDITSTTPERRLLVIRKQKGGRN
                     NQGKITVRHRGGGHKRYIRIVDFKRDKFDIPGQVMTIEYDPNRGARLALVRYADGEKR
                     YIIAPVDLQVGQTILSSKKLIEIKTGNALPLEYIPAGMPVHNVELRPGFGGKLARSAG
                     NALYVMGVEKKFAQLKMPSGEIRLVSKACLCTVGQVGNQDIARINLGKAGRNRHLGIR
                     PTVRGTAMNPVDHPHGGGEGNQPIGLKYPKTPWGKHALGVRTRDPKASSNYLIIQRRK
                     KKR"
     gene            73290..73574
                     /gene="rpsS"
                     /locus_tag="PKV49_00375"
     CDS             73290..73574
                     /gene="rpsS"
                     /locus_tag="PKV49_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPL22114.1"
                     /translation="MSRSLKKGPYINERLIAKIKKLAVGDKTVIKTWDRACTITPEMI
                     GFVIGVHNGRQHIPVLISESMIGHKLGEFSPTRKFVSHGGKMAKAQAKGK"
     gene            73601..73936
                     /gene="rplV"
                     /locus_tag="PKV49_00380"
                     /pseudo
     CDS             73601..73936
                     /gene="rplV"
                     /locus_tag="PKV49_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            74172..74861
                     /gene="rpsC"
                     /locus_tag="PKV49_00385"
     CDS             74172..74861
                     /gene="rpsC"
                     /locus_tag="PKV49_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPL22115.1"
                     /translation="MGHKVNPKIFRIGIIKTWPSKWFKQGQQYIKNLQQDVKLRDFLF
                     SNLRVAGLDRVEIERSANKVSVSLFVAKPGLVIGHGGTGAEDLKKKLHSQFLKNFRLH
                     DISLNIGEVNRPNLSANILAQTMALELEQRAPFRKIMKQTINRAERSGALGVKVMVSG
                     RLNGAEIARTEVLTSGKVPLHTLRADIDYARSTAYTTFGTIGIKVWVYRGEVFDNIEE
                     VKGEVVGQRLR"
     gene            74886..75302
                     /gene="rplP"
                     /locus_tag="PKV49_00390"
     CDS             74886..75302
                     /gene="rplP"
                     /locus_tag="PKV49_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPL22116.1"
                     /translation="MLMPKKTKYRKSHKAKRGGVATRGTTISFGSYGLKATTACWVTA
                     RQIEAARRVITRYVQRTGKLWIRIFPDRPVTAKGGEIPMGKGKGAVDHYIVPVKPGVI
                     MFEIEGIDEKRAREAMNLAAYKLPVKCQFVGRDQLN"
     gene            75321..75509
                     /gene="rpmC"
                     /locus_tag="PKV49_00395"
     CDS             75321..75509
                     /gene="rpmC"
                     /locus_tag="PKV49_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPL22117.1"
                     /translation="MEYRDLQTKSEHELQLMLAEKRAELKDLRFKNASHQLKAVRHIR
                     AVRQTVARLLTLLNNRQK"
     gene            75512..75784
                     /gene="rpsQ"
                     /locus_tag="PKV49_00400"
     CDS             75512..75784
                     /gene="rpsQ"
                     /locus_tag="PKV49_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPL22118.1"
                     /translation="MTKQNQVAAPKQAMRKKFRGVVVSDKMEKTIVVKVERVKVHPKY
                     HKRYTVSRKYKVHDEAGTCQLGDQVTFVECRPLSKDKRWRVLVNSR"
     gene            75808..76179
                     /gene="rplN"
                     /locus_tag="PKV49_00405"
     CDS             75808..76179
                     /gene="rplN"
                     /locus_tag="PKV49_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPL22119.1"
                     /translation="MIQVQTVLKVADNSGAKKVQCIRLMKGYRRHYASVGDMIMVTVK
                     EAVPHAAVKKSDVLPAVIVRTTKEIRRADGSYIRFDENACVIVDKKNHEPRGSRIFGP
                     IPREVRRAGFVKIASLAPEVL"
     gene            76198..76530
                     /gene="rplX"
                     /locus_tag="PKV49_00410"
     CDS             76198..76530
                     /gene="rplX"
                     /locus_tag="PKV49_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPL22120.1"
                     /translation="MKIKKNDKVLILAGKDRGKTGKVLQAFPRQQRASVEGINLLIKH
                     IRPQRRGEHGQRIEFPAPLHISNLQLICPQCGKPARVGYKYLAVKEGDKTKARKVRLC
                     RRCQQLID"
     gene            76544..77083
                     /gene="rplE"
                     /locus_tag="PKV49_00415"
     CDS             76544..77083
                     /gene="rplE"
                     /locus_tag="PKV49_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPL22121.1"
                     /translation="MRLKEKYNKEIIPALKKEFKYANLFDVPKLTKAVVNVGVGRFTK
                     DKAYIENVCETLAKITGQQPILNPARKSISAFKIREGMIVGVSVTLRGQRMYDFIEKL
                     VAVSLPRIRDFRGLNSKLVDRSGNLSIGIKESSCFPEIRSDEIEKSHGLEICLATTAK
                     TREEGLALFKLMGFPFKTE"
     gene            77110..77295
                     /locus_tag="PKV49_00420"
     CDS             77110..77295
                     /locus_tag="PKV49_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPL22122.1"
                     /translation="MARRALIVKAKKTPKFSSRIVRRCWRCGRNHGYMRDFGICRICF
                     RELASNGDLPGITKSSW"
     gene            77333..77746
                     /gene="rpsH"
                     /locus_tag="PKV49_00425"
     CDS             77333..77746
                     /gene="rpsH"
                     /locus_tag="PKV49_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPL22123.1"
                     /translation="MIDPISDMLSRIRNAAAVKKAEVVLPMSKLKYNIAKILEREGFL
                     AGVEIINPDSAKNKTAAFAELRLVLKYHNNGQSVITAINRISRPGRRVYSGKEKLPVV
                     VNNFGIAIISTSAGLLTNKEARQKGIGGEVLCSVY"
     gene            77770..78309
                     /gene="rplF"
                     /locus_tag="PKV49_00430"
     CDS             77770..78309
                     /gene="rplF"
                     /locus_tag="PKV49_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004059073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPL22124.1"
                     /translation="MSRLGKLPIILPAGTTAVLDKGILTVNGPKGQLQQAIHKFIKVV
                     IEDGAIMVNVDNKDERQQRAFWGLFHKLIGNMVIGVSQGYEKKLEINGVGYRVSIAGQ
                     KLTLNLGFSHPVDFILPDGIKGQVEANIITLTGIDKQLVGEIAAQIRRQRKPEPYKGK
                     GIKYIDEVIRRKAGKTSAK"
     gene            78331..78681
                     /gene="rplR"
                     /locus_tag="PKV49_00435"
     CDS             78331..78681
                     /gene="rplR"
                     /locus_tag="PKV49_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPL22125.1"
                     /translation="MSSTSKTAKKIRRHNRVRAKISGTADCPRLSVFRSNRGMYLQAI
                     DDTKGLTLASAHSREVKVKGNKTEVSQALGVLMAEKLLAKKISKIVFDRGSSAYHGRV
                     QAAAEGARQAGLQF"
     gene            78698..79366
                     /gene="rpsE"
                     /locus_tag="PKV49_00440"
     CDS             78698..79366
                     /gene="rpsE"
                     /locus_tag="PKV49_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPL22126.1"
                     /translation="MTEVKDTIVSEKQAVPEAVSAVTENQATERPSRSSNRGQRGNAG
                     GRGRGDRRSNDRQSQDEFDQKILDIARVTRVMAGGKRMRFRACVAIGDRKGRVAIGLA
                     KGLDVTIAVNKAVNQAKKNLVTVPMIDKTIPHEIKQKSDSAVIILKPAKQGRGIIAGS
                     VVRIILELAGINNVTSKMLGSHNKINNAKCVIKALRNLKKPAANHRSVSKPAVAIKTE
                     TGAE"
     gene            79388..79825
                     /gene="rplO"
                     /locus_tag="PKV49_00445"
     CDS             79388..79825
                     /gene="rplO"
                     /locus_tag="PKV49_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPL22127.1"
                     /translation="MLTLHTLKPAIGSSKQRKRVGRGNASGHGNYSGKGMKGQKSRSG
                     VSGIKRMALKKAMLQIPKLRGFQSNKPKYQVVNLSTLSRSFADGAIISADSLLKAGLV
                     SQAKNIKILANGELKLKNLQIKGLKLSAAAQEQLTKMGGKIVE"
     gene            79835..>80516
                     /locus_tag="PKV49_00450"
     CDS             79835..>80516
                     /locus_tag="PKV49_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPL22128.1"
                     /translation="MEKLLKIWRIRELRNNILFVIAMLVIFRLAAHLPVPGVNAQALR
                     DLFASNQFLGLMNLFSGGGMQNFSIVMMGVAPYITASIIFQLLGMIVPSIEEMNKEEA
                     GRQKINMWTRWATVPLAVIQSYGMIALLRRSSYQILGSIDWLNYVTIIVTITAGTVFL
                     MWLGELITERKTGNGISMLIFAGIIAGVPQSIQQLILTYDSSKLFMVIGFVVIAILTI
                     VGVIYISEG"
BASE COUNT        21226 a        15676 c        19117 g        24497 t
ORIGIN      
        1 taatcagatt tttagcacta gcacgactcc gcccactggc tatacccgaa gaaatatttt
       61 actaggagaa aatgactgtt ctgataatca taaatgggct cgaccgggcg cgtcacttta
      121 ttcggccgct tcttttgtta atcaagttga tgtaggttta gattatgatt ggaattgtga
      181 tcacaacgaa gaagttgacc cacgccaaga tttgtcttgc aatagcacat ccacatattc
      241 tagatatcaa tataaacttt gtaaggacta tgatactggt ggttgttgtg gtagtggtgg
      301 cggtggtaac accaaggcgc caaattttca atttgctcgt attgctcaag ctgatgatgt
      361 ggcatgttgt aatggccgac aaacattaac tggttgtttt ttaagtaata caaaatctat
      421 tggtagcgat tcttgtggtc aagagagaga ttttgcttgg gatactagtg ttatgcctga
      481 aaatagcact tcttgtgtcg gtggtagact aaattttcag gtatatagct atggtaagat
      541 tggttgcaga tagaatatta atatgacaaa agcaactgcc accaaatcat tgcgtattaa
      601 tatcaccact gttaccattg ttaaaacagt ggcgatattg ctgttgctgt atttagcttt
      661 cttggttaaa gaaattttgg ccttagtttt tgtcgctttg atttttgcca gcgcttttga
      721 tccgtgggtt gattttttac agcgccgcaa gtggccgcga actttgagca ttagtttggt
      781 ttatttgata gttcttagtt tagtcacggc tatgatttat gttatggtgc caattttagt
      841 taaggaagct accgatttgg ccgccagttt tccctattat tttaataagt tatccgaaat
      901 tttaaataat ttgcgtcaag tttttggtaa ttatggcttg gccgacaact ttcaaagcaa
      961 tctagataac ttagccgcga ctttatcggc ctggatcttg caagtggtta gtgcggttta
     1021 tggcgctttc aatggcttgt tttcggtttt tttgattatg gtgctgacct tttatatggt
     1081 agtggaagaa aatgccatca aaaagattgt ttggtcgcta actccggcac gccaccaggc
     1141 ttatgctatt gatctagttg gccggatgca gctgcagatt ggctattggt taagaggtca
     1201 attgattctt tgtttagcta tttttctgtt aacttatctg ggtctgtcta ttcttggcgt
     1261 taaatatgcc ctattattgg ctttggtggc tggtcttact gaggcaattc cttatttagg
     1321 gccaatttta ggcggcgtgc cggcggtgct gttggccatg actcaatcgc cgcgattggc
     1381 tttagtggtt attgtgatgt atttaattat tcagcaatta gaaaataata ttttagtgcc
     1441 gcaaatcatg aagcgggccg ctaatgttaa cccaattacc agcattatcg ttttattggt
     1501 tggctataag attggcggag tggcgggagc agtgattgct attccggtcg ccacagcggt
     1561 tagtgttggg cttaaagatt tgtttaatta taaagaaaag aaagaattgc gcctggataa
     1621 ggccgcttaa tcagtcagtt ttatgaaaac catacttact ggcgatcggc cgaccggtca
     1681 actgcatatt ggtcatttat tcggctcatt acttaatcgg gtcaagttgc aagctgatta
     1741 tgatacttat atcatggtgg ccgatgttca ggctttaact gataatttta attatcccga
     1801 caaagtacgc cacaatgttt atgaagtggt tttagataat ctggctgtcg gcgtcaatcc
     1861 gcaccaggcg acgatattga ttcaatcaat ggtgccggaa attgccgaat tgactgtctt
     1921 tttttctaac ttggtaacac tggctcgcct agagcgcaat ccaaccgtta aaactgaact
     1981 ggaacaaaaa aaagctttat tcaaaggcgg ggtaacttat ggttttttgg gctatccgat
     2041 tagccaggct gccgatattt taaccttcca ggctgattta gtgccggttg gccaagatca
     2101 ggagccgatg ctggaacaaa cccgtgagat tgctcgcaaa ttcaatagta tttatggccg
     2161 gctttttcca gaacctaggg ctttgctggg tgattttcca cggctatccg ggctagacgg
     2221 tcaggccaag atgagcaaga gcttaggtaa tgctatctat atcgccgatg aaccggcggt
     2281 tattgacaag aaggtcaagg ccgccaagac tgatccacaa aagattcggc aaaatgatcc
     2341 tggccatccg ccgatttgtc cggtttttgg ttatcagcag atttttaatg cggctgaggc
     2401 taaaaatatt gctaaagaat gcaaggctgg ccgtcttggt tgcgtggatt gtaagcgctg
     2461 cctagcggag acgataacgg cttatttagc gcccttccag gaacggcggc gagcttatgc
     2521 cggtgatatg cccaaagttc gcgatattgt tcgcgatggc actgccaaag cccgtcaacg
     2581 ggctcgtcaa accatggccg aagtccgaga aaagatgaag attgattatt gatttatagt
     2641 tctcttttta tatcttccca actaattccc gagattcttt ctatttcttt taataaataa
     2701 aacaaaacaa ctttatttaa gcgttttgga atagtaattg atttttgggt tatttgataa
     2761 gttgccttat agtgactgcc tttaccgcca tctttattaa tcgtgaaacc gagtttttct
     2821 aaagctgtag ttaatttttt tcgcggcaaa gtgcctggca aatatttaag cgatggcata
     2881 ctccctctcc ttgtcgccag tctggtacaa gccagcttct ttggcgtaag tggagggaat
     2941 ttcaaaagag gtcaagatgg catctttaat gtttttatct aactcaccag tatcttttcc
     3001 cgaagtgata atagaaccgt agataaaatt actactgaca gccacaatta atccattttt
     3061 gcggtattgg ttaaatttaa tcgggccatt attcttaaaa tagcgataca tctctgctag
     3121 gccagtcggc atattgcggc ccgacgtcat tttagcggcc aagtctttag cttgtttgat
     3181 gattgacata ttctaagcgg tgggaggatg gtaataaata tattataaat cttttgttgg
     3241 gaaaaataaa caactattaa tcattaataa acctttttac ttccgttaaa tcagtttttt
     3301 ggagattttt ccgttcagcg gcggagaaat ttttgagcac ataatcaacc gtattggttg
     3361 gtttggtggt cggctggatg cccaaccgat agcggttgaa tttttgggtg cctaagtagt
     3421 caataatgga ttggacgccg ttatggccgg ctgaccggct gtcagtggac cagcgggctg
     3481 cgcccatcgg caaatctagt tcatcgtgaa tgacgagcaa aatgtcgctt aaatcattag
     3541 ccaataaatt ataataattt ataatagcgc taaccgctcg gcctgagtcg ttcatcatgg
     3601 tttgtggttt cactaataag gtgtcgccaa tactggccac ttcagctgac cagttttttt
     3661 tattttgcca gctaattttt tgtccagttt cggacgctag ttcagcggct agtcggtcaa
     3721 tcaatagcca accagcgtta tggcgagtct tggcgtattt agcgccggga ttaccaagac
     3781 caacaataat ttttttcata atatttaatg attgcttttg tgctgcgggt tgatttgtcg
     3841 gccgcgagcg acatggatgc taatcggtgt gccgataaag ccataggtgt ggcgcaattg
     3901 gttctggata aagcgtagat aagaaaaatg cagattatct ttaatgccga tggtgaccgt
     3961 aaaacaaggc gggccagctt cggtttggac aaagctataa atatgaggat gctttaagcc
     4021 tttgcctttg gctggccggt gatgcttaac aatcctggat agaaaatgat caagttggct
     4081 tttgctcaag ctgattgaac gttgagcacc gacttcaata attaagtcta gtattttctg
     4141 gactttagat gtagttttgg ccgagacaaa agcaatcggc gcaaaaccga caaacggcag
     4201 gcgatgataa atagcctcgg tgaatttaac cgtgtcgcgt tcttcaactt tatcccattt
     4261 gttagcggcg ataattaaac tttttttgcg gttgataatt tcttcagcca agctagcctc
     4321 ttgatgactg atcggttggc tgatgtccac cagtaagatg gcaatatcgg cttgattgag
     4381 ccgctgccgg cttttagcca ttcccatttt ttctaggggt tttttcttgc ccggatcttt
     4441 acgagcgata ccggcggtat caactatttg aataatttta ttgcgatatt taataaaagt
     4501 atcctgcggt tcacgagtgg tgtgaggaat ggcgctgact aatactcggt tatagcccag
     4561 taaagcatta agcaggctgg atttgccgac attaggcttg cccagcaaac agactttata
     4621 gtcaggttgt tcagcatagg cgactagctc tagctcattc tcattggttg catccggtag
     4681 tgaatcatgg ggcgcggata atttgttttt agcattgcgt cgctggcgat taacctgttt
     4741 gacgatttca tcaagcagat cgccggtgcc catgccagtg gcggcactaa ccggccacgg
     4801 ttcgccccag cccaaagtat aaaattcact gattttatct cggtggcgcg ggctatcaat
     4861 tttattaaca accaataaaa ttggtgtttc tttgggtagt aattttttgc acgccaaggc
     4921 caattcacgg tctggcggca ataagccggc ttgtccatca accacaaata ggaccacggc
     4981 cgcttttttt aataattgtc cggtttgata ttggattttt tcttctagtt cggtcaattg
     5041 tttcgatttt tttgggctga tcgtcaagta gatcaattca ttcaaaccag cggtatcaac
     5101 taactccaag cgtcgtcggt tccaactgat gatatgatga ttggcgtcac gggtggtgtg
     5161 atcaaggtca gctaccagcg cccgcggcgc accaaccaga cggttaaata aagttgactt
     5221 gccgacatta gtccggccga aaataataat ttggcttggt ttaatcggcg gcatagtttt
     5281 aagggttatt ggaatagtca aggccaagca gcagaataaa atccggttgg gcaatattgg
     5341 ggttttgttt gattaattgg ctggtttctg tcgccaacca ttgaggaata tcagcggcga
     5401 tgattgcttt ggtcttgtgt tgcaacaagg ctaaatcagc cggtttggcg ccataggtta
     5461 gatcataaat agtggtttgc gccagatttt tgtgttgagt attactaata ttggtgacgg
     5521 taaaaccata ttgctgcaat ctagttttgg ttcgaccagc taaaccgttg atggtcgtgc
     5581 tattgcggat atccagctta gcttcggctt tgttcggtag attgtccggg ctaattgttg
     5641 ccacggtggc cgtagtttta gttattgcgc tattattatc aacaagagac ggagcggcta
     5701 ctgtgaatga ttcattaaag cgactgataa tttggctaaa atcgccagtg actggcgtaa
     5761 gaatataagc gccatcagtg ccaatcacat cgcgcagcag ccccttgggc ccattgtcta
     5821 agatgatatt agtaatttgg ctttgattaa catctttggc cagttgccac acactaacta
     5881 attcatcagg ttgtagattg gtggtcacat gttcctcggc attgccgatg atattcataa
     5941 ttaattttgg tttgaatagg gtattggcgc tgaatagttt atctttaact gcttcaagta
     6001 ttttttgttg gcgcttggaa cgagcaaaat cagaaccctc aacgccaaca ccgtgtcgcg
     6061 agcgggcata cttgagagct aattggccgt ccatggtttg ccgaccggca tcaatatgta
     6121 aatgttcaaa gcggctataa tagtcgggat tattttcacg acccataatt ggatagcggt
     6181 aatcatctaa agtgttttcc acttccactt caatgccgcc caagtcatca ataattttaa
     6241 caaagccgtc aaaatcaact cgcagataat aatgaatcgg catttccaga atttcactaa
     6301 tgagatcagc cgtggcttgg ccaccgctgc cgggattttg ggcttcggcc aaggaattgg
     6361 cgctattgat ttttcgccaa ccataaccgg tcattggcac agttaaatcg cgaggaatag
     6421 acatcatgac taccttgtga gttgatggtt ggaagctggc caccataatt gtgtcggtca
     6481 acatgccgcc atcgtgattc ttgccaccaa taccaagtaa aacgaaatta atacgatctt
     6541 gatcttcacc tttaagaatt gttctttgac tgacacccaa tttaaattta gttaacatcg
     6601 acaggctttt ccaaaaagaa aaaccttggc tattggcggc tccgccactt tgagtggctg
     6661 gccaaatacg ccagcccaaa taagccaaca ataccagact gagcactaag aataagcagc
     6721 tgaagcgctt tttacgcggc cgcggccttt caatagctga tgattgcaag ccaactgttg
     6781 ctcgttcatt gcgccaatgg ttagctggct gcggttgttt attggctggc tcaacgacta
     6841 ttctaattgg ttcgctggct cggctggtcg gcattggcgg ctggaattca gttgggtact
     6901 caggttgatc gtcaaacagt tcagccagaa tatcttgatt atcattattt tgattaggca
     6961 taattagcgg tgtttatgac aaaaaatggt tttggctgag gcaaaaccac ttgcagtata
     7021 aaagattttt tctactttgg caacttgacc gctaatccga ttcggtgcta tattaagacc
     7081 aagcttttca tcaaaagcca attgttattt ttcataatat taaataaaat atgatattta
     7141 cttatgattt taaagctcgt cttcgcttgt ggggaatttc tttgttagag atagtcaaag
     7201 tcggtcttct ggctttggcg attgttttgc ctatccgctt gtttattgtt tgcccttttt
     7261 atgttaaggg tgcttccatg gagcctaatt tttatgataa ggaatatctg ctaattgatg
     7321 aaatcagtta tcgttttcaa gcgccgcagc gcggtgaggt ggttgtcttt cgctatccgc
     7381 aagatccgcg tgaatatttt attaaacgga ttattggttt gccgggcgaa actttaaaaa
     7441 tggatcaagg ggatgtttat ctcttagata agtccacaaa tgaatggact aaaattactg
     7501 aaacttatct gcccagcact gatcagactt ttgcccttga tagcaacgaa ttaactcttg
     7561 gtcctgatga attttttgtt ttaggcgata atcgcgctca ttctcgtgac tcccgttttt
     7621 ttggtccgct taatcggcgc tatatagtcg gccgagttat gctgcgcggt ttgccaatat
     7681 caagagcgca aatttttaaa acccccagct attaattggc gaattggtta aaattttaaa
     7741 ttttattata tatggtggaa aaaaataaaa ctactgtcgc tcgtccggtt aaacatgaag
     7801 cggcgcccag ttcttttcgt cgccttaaag gaatgaaaga tgttctgcct ggcgattatc
     7861 cggtttggga ttttgttttg cggaccgctt ttcgtttagc tgattttcat ggttttgaac
     7921 gagttgacac accggtctta gaaaacttaa gtctttacga gcgttctagc ggtcgaacta
     7981 gtgatattgt cactaaagag atgtatagtt ttgttgacaa gagtggcgat cgaattgcgt
     8041 tgcgccccga agcgactccg ggcgttgttc gttcgtatat tgaacatggt tggcttaatc
     8101 tgccgcagcc agttaagact ttgtggtatg gcccgctttt tcgtcatgat aagccgcagt
     8161 tgggacgcta tcggcagcac catcagatta attttgatat ttttggtgag gccagcccgt
     8221 tggctgatgt tcaggtcatt tttttagccc accagattgt tagtgattta ggtattaagg
     8281 ctcaagtaga tatcaatagt ctgggctgtt ctgattgccg gccgaattat attaacaagc
     8341 ttttggctta ttatcgtgaa aagaaacatc gcgatcagct ttgccaagat tgtcgcaaac
     8401 gtttgttaaa aaatccgttg cgagttttgg attgtaaaga gccggcttgt attaatgtcg
     8461 ctcgtgaggc gccgcagatg gttgattatt tgtgcgatcc gtgcaagcaa cactttgtta
     8521 agattctgga atttttagat ggcttaagtg tcccttataa tctcaattca caattagtgc
     8581 gcgggttaga ttattatacc agaacagttt ttgaattagt cgttcccagc cagacggccg
     8641 aacggccatt gtcattgggc ggcggcggcc gatatgatca attagtggaa tatttaggcg
     8701 gtcggccaac tccggcttgc ggttttgcct taggcgtcga gcggctcatc ttgcaactag
     8761 acgaaaatca tgttaaggta gcgaccagcc aagatcagcc tgacattttt ttagctcaat
     8821 tgggtgacgg cgctcgctta aaatcattat tattatttga agaattgcgg caagtcggtt
     8881 tgactatccg tcagtctttt actaaagata gtcttaaggc ccagttagct ttagctagcc
     8941 aggtcggcgc taagtatacc ttgattattg gtcaaaaaga gttattagat ggcacgatat
     9001 tgttgcgcga catggaatcc ggttcgcaag aggttttgga ttttaagaaa gtcaaaacca
     9061 ttatggctaa gcgtctgggt aaaaattaat cctttattta gtttaataaa gctagaaagg
     9121 taggtgtagt atggttgaat ttaaacgcaa gaaaggcgaa agttttgaga gttttttgcg
     9181 tcgctttaat aagcggctgc agcttgatgg taagttaatg ttggtgcgcc aaaatcgtta
     9241 ccacaaaaat gaacctaata agcggcaagt tcgtttaagc gctctagtcc gcaaagaatt
     9301 gcgggaaaaa cgagcctatt taaggcgaat cggtaaatta aaagatgaat ttcgccaatg
     9361 gtaaaatgtg tttcaccagc taccaattaa aaaccgcggt ttcggccgcg gtttttaatt
     9421 ttgtcctttc tttttttgtt ctcgttgctt ccaccagtca cgcagacttt gtaatttcgc
     9481 tagtctaacc agcgccgatc gatgggcata ttggcggata gcggctttgg ctttactggt
     9541 tttaacgaat ttgagccaag ccaagtccgg tccggcctgt tttttgtcaa taattatttc
     9601 cactaaatca ttattatgca atatttcatc aaggcggcca atcttgttgt taattaagac
     9661 acctttagcc ttgttgccaa tttcggtgtg gacagcataa gcaaaatcaa tcggcgtcgc
     9721 gccgagcggt aattcatgga catcgccccg cggtgtcagg acaaataggt ggtcgttaaa
     9781 aatatccagc tgtaattttt gaataaaggt ctgtgtgtcc ggattatctt tttgtagggc
     9841 taaaatttct tgtgtccagt gcaagttcgg ggttaattga tatttattgc cctctttata
     9901 gttccaataa gccgccactc cgtattgagc ctgttcgtgc atctgcgggg ttctgatttg
     9961 aaattcagtc gctcggccct cttcgccaaa aacagtcgta tggagacttt gatagccgtt
    10021 aggttttggc aaggcgatat agtctttaaa gcgattatta tttggtttcc ataaactgtg
    10081 gataactcca agcgtccggt aacaatcatc actagtggga acaataactc gtagggcaaa
    10141 aacatcatag atctcatcaa atttacgttg tttattgttc attttttgcc agatactata
    10201 aagatgcttg aatcggccgt taatttcgtg attgattttt aattcatcca aagccgtccc
    10261 cagttcacgg ccggcgcgac tgatatattg ggctaattcc aatttttttt ctacctcata
    10321 tttttgcagc agctcttgat attcatgtgg atgcaaatgt ctgaaacaaa tatcttccat
    10381 ttgccatttt aggtgccaga tacccaatag gccggcaatt ggcacataaa tttccaatgt
    10441 ttctctggcg atgcgctgac gcttttcttc cggtaaatat tctaatattc tgaggttatg
    10501 cagtcgatca gcgaatttta tgagaataac ccgcaagtct ttagccatag ccaaaaacat
    10561 ttttttcaag ctttcccgat aacgttcttg gccgcggtag ctgattaaac ttaatttagt
    10621 tacactgcca accagaaaag cgatatcttc accaaatagt tttttaatgg tgggtaattt
    10681 atatttagtg tcctccggca catcatgcaa taaaccggca gcgacaatcg gggcatcagc
    10741 gttaagttga gctagaataa aagctgtgtg aagcggatgt tgaatatatg gttcgccagt
    10801 cagtcgcttt tgtccgtcat gagctttaac ggcgaagtta taagcttttt tgattaaagc
    10861 tatgtcggct ggcggataat ggtcggcaat cttggcaatt atttgttcaa tagtcataaa
    10921 caatcggctc ttgttggcta aggccagctt gtgctatact aaaactatac tttaattttg
    10981 ttaatcagtc aaaattaaca tgaaaaggtt atcatttagc gttacctggc tgctagccag
    11041 tgtttgcttg ttaattattt gtttaactcc actggcgttt ggcgttccta atgtcttagc
    11101 tgaagattct ttaggagtgg gctacatgaa tgataccgga ttatctgctc aagatccgcg
    11161 cattaccgtg gcgcggatta ttcaaatcgc tctaggtttt ttgggaacca tcgccgttgt
    11221 tttggtcatc tatgccggtt ttttatggat gaccagtgaa ggcaatactg acaagctgaa
    11281 acgggctaaa gagattttga aatcagcggc cattggtttg atgattattt tatcggcctt
    11341 tgctatcgtt acttttatta ttgataaact taatgatgct cttggacatg gctctggcca
    11401 gtcagtcgtt gatcgcccct ctgatgaaac gggcggttta ggcgctttag gtagtggcat
    11461 tattcagagt gtttatccgg cgccaggtca gactgatgtt ccaaggaata ccggcgttat
    11521 tgtgactttt cgggaaaaag ttaaagcatc aactgtctgt cagggcggca atgattcaac
    11581 tggtgtttgt aataatgcca atattgtcac tagtagtatt aggttttttc agaccgaagc
    11641 cggtgataat gaggccacta atttggctgc cgataaagtt aaggttagca gtactgatgg
    11701 caagactttt gtttttgccc caaccgaata tctgggatca gctagtacta atatttggca
    11761 atcagtcaaa ttaaccactt ctgtgcgcaa ggctaatggc cagccagctt ttagtcttgg
    11821 tgattttgtt tggcaatttg aaatcagcac tcgggttgac ttaactccac cgcaagttca
    11881 acttggcggc gttaccccat tccctgacaa cagtcaggat actaatcatg gcgttcaatt
    11941 agcacaacca gcgactgcgg tgattgatgt gttattacag ccgcagaccg cagctgcagc
    12001 caccgctggt acaccagagc cgcttggttc atccggcgcg gctcgcctgg agggaacata
    12061 taattgttcc ggcagtggaa ccatccgtgt tgttgcttca ggaacttcgc cggttaaagc
    12121 tcaggttcag ggtggaattg ctggtttaat ggatgataat gatgtcagtg atggccgggt
    12181 tagtttaggt tgtggtttga ctttaattaa gaatggatct atcgctcccg ataaccacga
    12241 aaccattgct gctggcaata tttgggatat tgaagtggcc gcggcgcggt caggtgatag
    12301 cttgaccgtt ggtaaccaaa gttatatttt tgttaactcc accagcactc atcctaatca
    12361 tattgtagtg gctagttctt tatcagctac ggcggataga attgttgagc gtattaatca
    12421 gaccaatagc gatgttagcg ctgctcggca atcagccagt aataataaga ttgttttaca
    12481 agctcgtttt gccggcgagg gtggtaattc tttagtttta ttggataata gtctaggaat
    12541 ttctattgaa tccggcttta gtggcggaca gaatcgtcaa gaacaaataa ccgttaatgg
    12601 gcttaatgat tcgccgcgca attctatcat tcaaattaat ttttccgaac cggttagtcc
    12661 gctaacctta gttggcacca gtcaagaggt tagctccacg attgaagtga taaatgttaa
    12721 taataacagt ccagttagcg gtcgttttgt tatctctaac cagtatcaga cgctcgagtt
    12781 tattcctagt gaagaatgcg gcaccaatgc ttgtggcgac ctgatttatt gtctgccggc
    12841 caatagtcag ctattggtta gaatcagagc ggctaattta cagccctgtg ccgctagcgc
    12901 cgattgttta gataagacac catataatca atgcagcggc tcttgccaaa aaacagctga
    12961 tgatcattat ccgctggccg ctctgccatt gactagcggt ttagttgacg ccagctttaa
    13021 ttcgcttgat ggtaatcgtg atactaaggt tgtcggcccg gttaattttt atagtgaaaa
    13081 tactcaagat accaatcagc gggatagcta ttcctggtca ttcttcgtta atgatgatat
    13141 tgacctgacg ccgccgcggc taaccgctgg atcattatcg ccgagtttgg ggttggccac
    13201 ttcttcgctc aaccagttga ttgagtttag ttttaataaa ctgatgatga tcagcacctt
    13261 aaatagtggc gaggtattga ttaataacca taaccatcgt ttgattaatt tattcagcac
    13321 cggtttagcg ccaggctatt ggttaaccag ccgagcagtt gactttggcg tgcctgatgg
    13381 ttatcctgat tttactaccg ttacggttaa tcatgacctt ttggccgatg ccaccaggta
    13441 ttcggtggaa attggctcgg gggttaaaga tttgcgacaa aattgtttca agccatgtca
    13501 ggatgacgct gcctgcagcg ctactgacgc ttcttgttgt aatggctcgc cgagtagtgg
    13561 agccacttgt aattaataaa acaccattat gaccaggggc caaaaatttt tactaagcgg
    13621 attattaatt ggcggtttag ccgtcgctgt ttggtggggc ggtcaaactg ttttagctca
    13681 gacaaccacc gatcttggtg ttgattatgt ggccgcttcc ggcttaagcg ctcaggatcc
    13741 gcggctgacc gtcgctaaaa ttatcagaat tgctttcggc ttattaggca ctattgccgt
    13801 tgttttagtc atctatgccg gcttcttatg gatgacggct gctggtaatc aggataaaat
    13861 tcagcgagcc aaggatattt taaaggcggc cgtcattggt ttagctatca tactgtcagc
    13921 ttttgccatc accaacttta taatcaatca attgcttgaa gcgactaatg gacctggtgg
    13981 cagcggttct gttgatccag aaataattta cccgccggct gatgctgatt ttatggtcac
    14041 cggctctagt ccggctaacg gatcaattga tttgccgcgc aataccttag tgcgttttca
    14101 atttaacggt tcaattaatg ctaacagtgt taatgctggc acggcccaga ttctggctga
    14161 tggcagtcca gtggctggtt cgtggcaggt caatggccaa gagatgtttt tccaacccga
    14221 cgctatctgt aacggtagct gcggcgaggt taattgcttg ccagaaaatg gcacgattag
    14281 tgttgagtta actagtggta ttcagagcgc cggtgctaat cctaaaaatt tagtctgcgg
    14341 ttcggctatt ggttcggcaa cttgcagtct taacttcgcc gttggctcaa ccattgattg
    14401 tcaagatcca acagctgaat ttatttctag tgtcggtatt tgttccggcc ataataataa
    14461 tatccgggta gccgccagtg atgatagcag cttggctagg tttgaattct tggctaataa
    14521 tcagtctttt aatctgcaga ctggtaattt tggcaccagt tttgtggcta gcgctttatg
    14581 gacgccaacc agcagcgctg gcgctaattt tactttacag gcattagttg atgatttagc
    14641 tggtcgtcgg accagtgttc agcaggctgg taaattatta ccggctcatt gttgtaacca
    14701 aactcaagat ggcgatgaaa ccggcattga ttgtggcggc agctgcttgg gttgccaggg
    14761 cgcggcgtgc gctactgact tacaatcgcc agcagccact tgtaataata atttatgcag
    14821 ctcgggtttt tgtactcaac aaggctctaa tgcatcagct tgtcaggcgg ctggctatgc
    14881 ggctggtacc gcttcttgtt gtttgtgtaa aaataaaccg attattgcta gccttagtcc
    14941 acaaggtggt ttttgtgtta ataacactga tcagtattgt ttaattaata gcgattgtgg
    15001 aactggcgga gtttgtgatc aaaattcgcc caatgccaag actggtaatt taatgactat
    15061 tattggtagc ggttttggca cgactactgg gtcggtggtt tttgctgaca ataaacaggg
    15121 agatttattg gtctgtggtg tcaactcttg gtctaataat cagattgttg tcaaggtgcc
    15181 gactggggcg attactggtc cggtcaaagt aaccaatagc gataatttta gcgccactag
    15241 cacgcaatca gtggtaatta ataatattgc tcggcccggc ctttgctctt taagcaagcc
    15301 aaccggcacc actaatgaat tgattaatta tcaaggatta aatttcagta atagcggacc
    15361 cgcggttacc aaggctcttt atggtaattt aaccaatccg attccaacta atgagtcgca
    15421 agtccttagt aatgttttga ttaaagcgcg ggttcctagt ttacagcctg gtcaagttac
    15481 tacttttgtg cgcaacgagg ggactactat tggtccggcc agtaattatt tatcatttaa
    15541 taaattagct gatccaggca gtcagccgca gataaccggt tttgaaccgt ccagcggacc
    15601 acctggccaa tatgtcacta tttatggcca gggttttggt aatttgcgag ccggtagttt
    15661 agttaaattt ggcagcattg aagctaatta tgaatttcca gctgaatgcg ccaattcggt
    15721 ttggtcagac cgacaagtta ttgttaaggt gccaaataat atagccgttg gcacatataa
    15781 cctgtcaatt agtttagcta gcggcgcagt cttggcggct actgatgttt ttacggtcaa
    15841 caccaatcca ttagctccca gtatttgtcg aatcagtcca accactgctc gtgtcggtag
    15901 tcaattacaa gtttggggtg aatattttag ggattatgat gccgcgacca gtcgactgcg
    15961 ttttagtcag aatattaatg ccaccagctc ttggttttat caatcaagtg ataattttgg
    16021 cagtgcttca tcaacaattc cggtgggcgc tgtcagtgga ccagtaagag tggttaatgc
    16081 caatggtgtc agtaacggcc ttaatcttaa gattggtgct tgtcagattg atagtgattg
    16141 tcagggccag atttgttgcc cagcgtcatc accgcggggt ggtcagtgta taaccggcgg
    16201 acagccagcc tgttacggta cttcggctag ttgcgtttat cgttggtcat tcaatactgg
    16261 cagtggcaac caagcttgcc aagacggcac cactctttgt ggtaatcaat gttgtgacag
    16321 cagccaggaa tgtcaggata atgtttgcgt taataaagct ttgttatctt gtaatgatta
    16381 tcatcaatgt aatggagctt tatattgccc taattcaccg ggttggtgca gccagaaatt
    16441 gcatccaact atcaatggtg gttgcggcga tgattattgc cgcggattat ttgtggaaca
    16501 agggttatca ccggattcgg ttaattattg ggctaatgct aatgtttgcg tcaaccaaca
    16561 agaatgtgat ccggctaaag ctgttgtttt ccaagttggt gttaatcaag taacctatca
    16621 attacactgt caagatggct attggacaac taatttaacc gccggacaat gtccgcctgg
    16681 tcaaactgat tatggtcagt ggcaattatt agctaataat atttgtcgta gcaataggcc
    16741 ttgcagtcaa tgtgctaatc atttatcttg cttaactttg caaaatcaat cttattgcgg
    16801 tcttgaccag aaggtttgtg ctagcggttt tacttgtcag gataatcagt gtcagaaagc
    16861 ggctgattcg tgcgaatgtt gttgcgataa gagtttaaat aatccaatca gcaaaatcaa
    16921 tcccggctgt tgctctccgc tgacttgtac tgatacttgt ggtgccggcg atgtccaggt
    16981 tagcattgat ggtgtaacta ctactaaaca atttggagtt tgttccggtt gtaaagaagt
    17041 tagcggcggt caagctgaac gggatgccgc ctgtaattgc actggctcaa ctggtaagta
    17101 ttgtgatact tccaccccat tgggcacttg tcgcgactgt tcacagattg ccaatgctgc
    17161 cgaatgcgct agccgcgctt cttgttgttt tgatcagaag accaataaat gccgtggcgt
    17221 agtgaataat gccaaaataa tctcgggagc tgatgccggc aaatgcggtt actatcattg
    17281 tgatggccaa gcctgtgccg ctgaattaac agccagcggt cagttcgcca ctgctaccga
    17341 gtgtgaaact aaatgtcgtg ttgttagtct tcaaggtcaa tcttgtaatt tagctaccac
    17401 cactataatt agcttgaacc cctataataa tttacctaat actgagcaaa ttggttgcgg
    17461 ttatcaatta tgcgccgcgc ctttaagctg ccgagtagct agtggtacga gcacagctac
    17521 taataatcca gccgattgcg gaatttgttg ctgcgaccct agtcatgata cttgtgctca
    17581 attgagtacc aacttatcgt gcgtggctaa tcgtggtcaa tgttctggat ctgggcgggg
    17641 cttgtgttgc ggttgtcaaa gtgatgttga ttgcggcgat aatcttaata atggttgcgg
    17701 tcgcgatact tgctgtcagg ctcgtccgat gttagtagat actttgcctc aaaatggcga
    17761 taatggagtg tgtcgcaatg cggttattca ggccagcttt aatcaagcgg ttgatttagc
    17821 tagcctatca gataatcttt tattgattgc tgattatggc aataaccggg aatgccctga
    17881 tgggaccaaa tatttagcca tggattctgc taagccgatt agttggttag ctcgtctgcg
    17941 ccaaagaatt ttggcctgga ttaagccgag cgctttggcc gtcttatcaa cagctggcaa
    18001 tacttattgt tctattaatc gtgaaataag tgtttttaat caagatgtga ttattcgtcc
    18061 aaccagtgtt ttagatatga atcggcgtta ttttgtggtt atggtcaccg atgctgatat
    18121 caatgataat cggccagccg gaattttgaa ccaagctggg atcggtatgt ctcgaggtca
    18181 gccgctacga tttaataatt tgcaatttcc agcagctatt cagtggtcgt ttactactgg
    18241 aaaacacatc tgtcaaattg atcacgttaa gattgatcca aattcagtat tactatctaa
    18301 tttagaggat tcggctaatt taaacgctac tgctattagc gctaatggcc agcctttagc
    18361 cactagcact aattataatt ggcaatggtc atggttagtt ggtaatcagt cggttgctca
    18421 gttggattca gctgataaca aagcgattgt ccgaccgcaa aacaagaaag atgccgaaac
    18481 ttatgttcga gctaccgctc aagtgactgc tgatcattca actggcgctg gcactagcca
    18541 aactagtttt tctcaagaag cagccattaa agttttcctt tgcgccaatc cttggccagc
    18601 ccgtcgcagc gacggtagct ggcggccttg gaccgattat gccactaata tgcaattcta
    18661 ttattgccgt gatgggcaag ctaataccca gtccagcgat ctgcccggat tgaaggtggt
    18721 tagcagtact gatccgacag cgattaatcc ggccttctat tttttccgtt acaaccaagc
    18781 gactacctca ccggttaatt tacaaggaac tgcttcgcca gctggtcaat cggttagttt
    18841 aacctggaat gcttacccca gcgctgtcgg ttataaggtt tattatggtt tacgcagcgg
    18901 ccagtacttt agcgatccaa ttaatgtttc gcaagatact ctaactacta ctgttaacga
    18961 tttaattaac ggccagctat attactttgc tgtttctgct attttacaga ttgatcaagc
    19021 tacttttgtg gagacaccat tatctgcgga aattcaagtt cgcccgctag atatcacagc
    19081 tccagctgcg gttcgctgtg ttgttcagaa tattgttaac aataatatta atttagaatg
    19141 gcggcgaaat actgatgata ctgtggccta tcgtatcgaa tatcgggcta gcggagccac
    19201 tgcagccggc aaggtgatta ttattaatca gccggcatct ggccggactg ttcatggagt
    19261 gattcctgat ttagttaatc ccgagaatgt cttgattgat ataaaggctg ttgacttagc
    19321 tggcaatatt agcgcgcgac agaacgaatc gctggttggt tgttttaatt aatcgctgat
    19381 ttggttgata atagcctttg tgatataata atttagctta tttataaaac taaacttatg
    19441 tttgatgaag tgaataacca gcctactcca gcatcggcca gtcagccgac tgacaactca
    19501 atgcccaacc aggctccagc agcgccaacc aatcaggcca ccgatggtca agtccaagat
    19561 atttttgcta atggtgaatc accagcggct aatcagccca gcggtcagcc caatttttct
    19621 caaccattta tgccggccgg actaccggta actgctcagc cagttaatca gctggtgccg
    19681 aaatctggat ctaacgctaa gttaattttg gttgtcttag ttattttgat tttagctggt
    19741 gccgccgcct tagtttactt acaatatttt aaaaagactc caccgccgat tattactcca
    19801 gaggctatta atgtgaacag cgaaactgag gttactacta ctgatgaggc catggctacg
    19861 gccactgatg aaaccattgt cagtccgacc agtagcgata atccggttgg tgttgacata
    19921 gatactatta atgtttttga tgaaaatgtt agttcaaccg aattagatca aacaacaatc
    19981 atcgacagtt caatgcctga gcctgattct gaagctatcg ccaatcttga tagtgatcag
    20041 gatggtctaa ccgatgccga agagatcaat atttatcaaa ctaatcctct ccaagctgat
    20101 agtgacggtg atggctatac tgatggtgac gaggttagag ctggctataa tccattaggt
    20161 agcggcaaat tagtccagta atcattaggc cgctgatgtt ttaattatgt cggaccaacc
    20221 gactaaaaat tttaaaatcg cctattccga cgaaactggc gtctctaccg accagattga
    20281 ttttgttaaa caaagtgatg tcggtgtcgg cattgaggct ggtcagtata tttacaccat
    20341 gcctaaggct tttcggtttg gaccaaaagt taaaacaaac aataaggttg gaattttggt
    20401 tatggtggcc gggttgattt tgtttgttgg tttagtttac gtcggttatt taatgcttag
    20461 caagaagccg atttttagct ttcggccggt ggcttctgac aataatccaa cccccattag
    20521 tgtagacaat caagcgccga ccacaacgac caccactgaa ccaataacag aggtcgcgat
    20581 tgatttaagt gatcctgaac aagcttattt gcagttgcgt ttaaaattag atcaagcagc
    20641 cacattagaa gagtatttga attcttttat cgccggtgcc agccaaaata aggcctatca
    20701 attagctgaa cagaccattg gcttagacgc tttatcagat agtcaaaaat ccagtacact
    20761 cagtattagt aaatcaatgt tggtaccgct gtcatcagct gatcaaatca ccaaggatat
    20821 tagcggtgac cgggcagtat taactatcac taaagccgat actggccgga tcgccatcgt
    20881 gacgatgttg ctggaaaacg atcaatggaa gtttgatgac gagcattggc aggatgctgt
    20941 taatttatcc gttagtacca ctactgataa tttaataata ccatcttctt ctagcagcac
    21001 tattgatcta gttgaagagt tttccttaga cgctgatact gatcaagacg gattgaccga
    21061 taaagaagaa ataattctgg gcactaagat tgaccaagcc gatagtgata atgatggcta
    21121 tgatgatcta actgaattgc gggctggcta taatccagct ggtagtggca agattaccaa
    21181 taatagcggt ttaaaggtgg ttaaacaagg tcgttggtca accatagtac cagccgcttg
    21241 gactagccaa ttgggtcgtg atgaatcggc tattttccga actgacgatg gtcattttat
    21301 ccaagcagtt atcctcacca agaagaatgg ccaagatttg gctgattggt atcaggaaat
    21361 ttttactggt cagactgctg atgtggttaa tgttggtcgt aatcagctag ctatcagtct
    21421 ggacgggcta acttattata ttggtcgacc ggatttaaat tatttagtca gtttaaccta
    21481 taatcctgaa accactaagt tattgagtta cagcaatatt tttcgtttag cggccgataa
    21541 tatgacactt agcgattaaa catggtaaca gcttgtaatt taactaaaca gaatattgac
    21601 ctagaccgtt ggcaaaagat tagtcagtta tttttacaag aacaacaagc agatggtgat
    21661 attagtctag ttctagttgg cgatcggcgt attcggcatt taaataatca gtaccggcat
    21721 cgtgaccaga caaccgatgt cttatccttt actggcgatg gcgacggaca attaggcgag
    21781 attatggtta attatcagca aattacccgt caggcccggc gtttcggtca ctcaacttgg
    21841 cgagaattaa tttttattac cactcacggg attttgcatt tattaggcta tgatgatgat
    21901 actgatggcg gctggcaagt tatggagaaa ataagtcaag aattcgttga gaaatataaa
    21961 atatgttgag ttatcgtcgt ttattaaaaa gttttcgtta cgcccgccac ggtttatggc
    22021 aggttgccaa agaggagcag aattttgcca ttgaattaac gattggcgtt agtgcgataa
    22081 ttttggccgg cctgttgaaa tttgctttgt gggaatgggc gattctatca ttaactatcg
    22141 gttttgtctt aattaccgaa ttgtttaata gcgccgtgga gagaattagt gatttattaa
    22201 aaccgcggct ggatatttat gtcaaagaga ttaaggatat tatggccgcc gcggttttct
    22261 tggccgctgg tttatcagtt attgttggat tgatattatt tttgccccat ttgattaaac
    22321 tattaatcac tatatttcaa taaagcctat gcgcgatcaa acattagtgg ccggattgga
    22381 tatcggctct acggaaatta gactagttgt cggtcagacc actggcgggc aactgcaaat
    22441 tattggcgcg gtgtctgctc cggcggcagg tattagcaag gggattgtca ataatattga
    22501 ggaggttgct tcttcggtca tggcttgtct agaaaaatca gaacgcttga ttggcgtccg
    22561 agttgatagt gtttatatcg gtgttagcgc ccctagtttg aagtgtgagc gcagtaaggg
    22621 cgtggtggcg attaaccaaa tgaataatga aattactgat gatgatgtta ctcgagtcat
    22681 tgaggccgct caagctttat cgttgccggc taattatgaa gttctgcatg ttatccccat
    22741 taaatatcgg gttgacaatc aagatgatgt taagaatccg gttggtatgt cgggtattcg
    22801 tctggaggtt gaggttttaa ttatccagtc tttgaccaat caaattcgta atatgaccaa
    22861 ggcgattgaa caggctggcc tgtcaattgt tagtccggtt ttagcgccgc tagcggcggc
    22921 cgaggctgtg gtcagcgctc ggtccaaaga gctgggggtt gctttagtta atattggatc
    22981 gctaaccact agtttggctg tttttgaaga agggcaatta ttgcatgccg ccattctgcc
    23041 gattggctcc gatcatatta ctgccgatat tgccattggt ttgcgctgtt ctattaattt
    23101 ggccgaaaag attaagctag aatttggcca agccaatccg gagaaaattg ataaagatga
    23161 cgaggttgat attacgccgc ttatttctcc agataacgaa gttgatgagc cgatgaccgt
    23221 atctatgaag tatctgtcgg gtattattga agcgcgagtc agtgagattt ttcataaagt
    23281 tgatgtcgaa ttaaaaaaga ttaatcgttc cggcatgtta ccggccggtg tcgttctttt
    23341 gggtggcggt gctaagttgg ctgggttaac cgatttagct aaaaagaatt tgcgtttacc
    23401 ggtctgtttg ggagctaatc gcaatatcac tgccgtcatt gacaaggtta acgatttgag
    23461 ttatttgact gctctgggct tagtggcctg gggtgatcat tatgattttt taaccgataa
    23521 cgattcagtc agcagcggat ttaagcctaa gcaggtgatt aagcgagcca aagattggtt
    23581 tgggtcgtta tttttaaagt agtttttctt gattttttta gccagataat ttacaatcaa
    23641 agcaaacata ctatttaatt ttatagctcg ttagctaata ttcttatgac agaaatcaag
    23701 ccagcagttg aggcgccagc caaaattaaa gttgtcggtg tcggcggttc cggcggcaac
    23761 gccattaacc gcatgattga gggcggtatc aagggtgtgg aatttatcgt tattaatacc
    23821 gatattcagg atttgaatta taataaagct ccaatcaaga ttcacattgg aaaaacagtt
    23881 accaaaggtc ttggcgctgg tatgaatccc gatttgggcc gcgatgctgc agccgaatcg
    23941 caaaatgaaa ttcgtgacgc tctcaaggga gctgatatgg tttttgttac ctgcggtttg
    24001 ggcggtggca ccggtactgg cgcttcgccg gtcgtggccg aattggctca cgatctcggc
    24061 gctttgacta ttgctgtcgt cactaagcca tttacttttg agggggccca gcggcgcaag
    24121 attgctgaac gcggacaagc cgaattaact gacaaagttg atgctgttat tactatccct
    24181 aatgatcgta tttttcaggt aattgataaa aaaacgacta ttctggaagc cttcggtatt
    24241 gttgacgata ttttgcggca aggtgttcag ggaatttcgg agataatcaa tgttcccgct
    24301 ttaattaatg ttgattttgc tgatgttaag tcaatcatga tgaatgctgg tagcgcttta
    24361 atgggtatcg gtcaagcttc tggtgaaaat cgcgccattg aagccgctaa agccgctatt
    24421 aattcgccat tattagagat gtcgattgat ggcgccaagg gtgttttatt tacaattact
    24481 ggcgggccca gtttggcttt agatgaaatt catgaagccg ccaaaattat tactaattcg
    24541 gttgatgagg aagcgcgaat aatctttggc acctcggtag acaacaattt taaagacgag
    24601 gtgcggatta cagtgattgc taccggtttt gatagtcgtt cagctagtaa tgacaatcaa
    24661 gctatcggca agagctacaa tcatttttat aagccgacga cttttgttga aaaaggacaa
    24721 gatgatgatg ccaatgaaga agtaaaagaa aaccgtaaag gattgatgtc ggctcttaaa
    24781 aaggcgcaat cagaaccggc tcgccagcca acgccgccag ctgcttccag ttcctctgtc
    24841 gacgaagatg acgacttaag tatcccgact tttatccgca aaaagttatt ataaaatgaa
    24901 taaaaattgt gcacaaaaaa tgccgctcat tattgggcgg cattttttat tattttttag
    24961 ctattttttt agctcttgct ggctttggaa aaagtgctat aatgagagca tcagacaact
    25021 acattttgtg gagaaacaac ttagtgaggc acaatatgta gttttagcca aaaatcatga
    25081 aatgtccgat ttgttatcat tctgagacca aggtagtaga ctcgcgagtc gccagcgatg
    25141 gcttgtcgat ccgccgccgc cgcgaatgtt taaaatgcgg ttttcgtttt tccacttatg
    25201 aggaaactga gattttagat ttattagtga ttaaacgaga tggtcgcaag gaggtttata
    25261 gtcgggataa gatgatttct ggcttgcgta aagccactga gaaaaggccg attaatagcg
    25321 atcagttcaa acaactggtt aacttaattg agcgcgatat ccaggcggct cgtcagcccg
    25381 agattaacag ccaagatatt ggccgtatcg ttatgaaaca gctcaaaaaa attgataaag
    25441 ttgcctatat tcgttttgct agcgtttatt ccgattttga attacaagat ttaagccaag
    25501 agctgaatcg gctgcttaag ttaaaaaagt aacctgcatc atctatgagt caaggtatta
    25561 gcaagatttg taaacgctcc ggagaaatcg ttgattttga tcagagcaag attaccaacg
    25621 ctattaataa agcaatgtcc gccgtcggtc aacccgacaa agccaaggcc gaaattattt
    25681 ccaatgaggt agttaaatta atcaaccaaa aatttcatga acgaagtatt ccggcggttg
    25741 aagaaatcca agatattgtt gaggaagttt tgattaacaa tcgtttaatc aaggttgcta
    25801 aatcttatat tctgtatcgc gaccagcatg ctcgtttgcg taacttgtcc ctgatgattg
    25861 attcggagaa aatcatggat gattatttaa aacaaactga ttggcgggtc aaggagaatt
    25921 ctaatatgag tttcagcttg cagggattaa ataatcacct ggcctcaatt atttcatcaa
    25981 cttattggct gaataaggtt tataatgcgc cgattcgtga agctcatcaa aatggtgatt
    26041 ttcatctgca tgacctacaa attttagcgg cctattgcgc tggttgggat attaaagatt
    26101 tattattaaa gggtttcggc ggcgttagcg gcaaggttga atctcgtccg cctaaacatt
    26161 ttagaaccgc tttaggccag attactaatt tcttttatac tgtccagggc gaagtggccg
    26221 gcgctcaagc tttttccaat tttgacacct atttggcgcc atttattcgc tacgataaat
    26281 taacttatac cgaagttaaa caggccatgc aggagtttat ttataatgtt aatgtgccga
    26341 ctcgggttgg ttttcagacg ccctttacca atattaccat ggatctaaaa ccgtccaagg
    26401 caatgggtga agaaaatgtt attattggcg gcgaacttaa agaagagcgt tatcgtgatt
    26461 ttcagccgga gattgatatg ttaaatattg cttttgccga attaatgatg gaaggcgatg
    26521 ccaaggggcg ggtcttttct tttccgattc ccacctataa tttaaccaaa gattttcaat
    26581 gggattcgcc tgtagccaat aagatttttg aaatgacgcg caagtatggc attccttatt
    26641 tctccaattt tatcaattct gatatggatc cggatgatgc gcggtcaatg tgttgtcgat
    26701 tacgattgga caatcgtgaa ctgcgcaaac gcggtggcgg tttgtttggc gccaatccat
    26761 taactggttc tatcggcgtg gtgactatta atttgccgcg tattggctat ctcagtcaga
    26821 ctaaagaaga gtttttccaa agactgggta atctaatgcg actggctcaa gagagtctgg
    26881 aaactaagcg ggagattatt gaaaaattaa ctgaaggcgg tctttatccg tatgcccgct
    26941 attatttggc tagcatcaaa gagcggtttg gcggttattg gaacaatcat ttcggtacta
    27001 tcggtttaat cggtatgaat gaagccttaa tcaattttgc gccgattgct aatagcattg
    27061 ccagcccggc tggccaccag tttgctgaag agattctgga atttatgcgc gaaactttag
    27121 gtaaatttca agaagaaacc aatcatttat ataatttaga agccaccccg gccgagggta
    27181 cttctcggcg cttagcgcgg ttagatcgtg atctttaccc caatatcaag gtagccaata
    27241 atgataaaat tgaccaggcg ccattttaca ccaattcaac tcaattacca gtcaatttta
    27301 ccaatgatat ttttgaagct ttggagctac aggatagttt gcagaccaaa tatactggcg
    27361 ggacagttct tcattttttc ttaggtgaat cattgcccag cgcttcagca gtgcggacct
    27421 tagtgcgcaa ggtggcggag aattatcatt taccctattt tactattacc ccgacctttt
    27481 ctatctgccc caagcacggt tatattgccg gtgagcattt ttattgtccc aaatgtgatg
    27541 aggagattgg ttttcacccc gaaccagcct tggccgagat ggcctgatta atttgtttat
    27601 taatttttta atccccatgc ttatgtctcc cttagaaacc aaacgcaccc cttgtgaggt
    27661 ttacagtcgt gttactggtt atttgcggcc agttaaacaa tggaatgatg ccaaacaagc
    27721 cgaatttgct aatcgtcaaa cttttattgc tgaaggcgag agtgatagct gcggttgcta
    27781 actatgatta tcggcggatt acagccagtt agtctgcttg attatcctga taaaatttcc
    27841 gctattgttt ttactcaagg ttgtaatttt cactgtcgat actgttataa tcccatgctt
    27901 gtccggccgg caacggttgg tgttaccaat accacaggtc agccagttta ttcgttggct
    27961 gacctgtggg cgtttttaga tcaacggcaa gatcgcttgg aagccgtagt ggtgactggc
    28021 ggcgaaccaa ctctccatca tgatttgccg gaatttatca ctgagatcaa gcgacgcggc
    28081 ttattggtta aactagacac taacggaaca gccagccaaa tggttaaaca gttggtgcaa
    28141 gacggtttaa ttgattattt agctatggat attaaggctc cttggaataa atatgatcaa
    28201 ataatgcctt taccggcgga tattttgagt cgcttaaccg ctgaagctaa aattacggcc
    28261 gagtggttat tgactgatca accattggat tatgagtttc gtaccactgt ggtgccacag
    28321 ttattatcgg cggccgatat tagcttactg gccgaacagt tgattggcgc tcgatcttgg
    28381 tatttacaaa aagctcggct ggctggtgat ttattggatc ctagcttagt ggggcagaga
    28441 gtctatactg atgctgaaat ggttgagtta ttagctatag ctaaagctaa ggtgccaacc
    28501 gcccagattc gttgattggt gagctaaaat ttttttgcta taatattgat atcaattagc
    28561 ttaatttatt ttatggatga agatttgcgg cgagttttag ccgaccaagc agcagccatt
    28621 gccgagattc gggaaaacac ccgattgatt tatcggtatg tttattggca aagaattctt
    28681 agctggatta aatggcttct gcttctcctg tctttattag ctggtgttat ctatttgccg
    28741 ccgttattaa aagattattt tagtttgtat aactcaattt tgcggtaatc ttatgcttga
    28801 aatagttaat ggtcaaaata ttgaggcggt ccgtcaacgc cttctagaga ttgggaggtg
    28861 gctttgacct agctgccaag cagcggcaac tagccacttt gcaagcaaaa gctcagacac
    28921 ccgatctgtg gtctgatcct gaacaagccg gccaattatt aaaaaggatt gaagttattc
    28981 aaaccgaaat taataattat caggccactg aacagcgttt agccgactta gccgccttag
    29041 ctgcgactgg cgaaatcgat gccatcactg ccgatttagt gcagactgaa ctgcaacaaa
    29101 ttattcaact tatcgagcag ctggaaaata gtcgtttttt tgatcagccg cacgatgatt
    29161 tgccggttat tttgtcaatt cacgccggca ctggcggtgt tgatgctcaa gattgggctc
    29221 tgatattaga gcgaatgtat ctgcgctatg ctgatcgaca gggttggtct agccaagttt
    29281 tagatgaaca ggccggtaat gaggctggca ttaaaagtgt taccttgcgt ctagctggtc
    29341 cgctggctta tggtttttta cgcggtgaag gtggcactca tcggctattg cgtaattcgc
    29401 cgtttaatgc tcaacatttg cgacaaacct cttttgctat ggttgaagtt atcccggaat
    29461 tgccgactat tgaagcgccg gcaattactg agagtgaatt acagattgat gtttttcgtt
    29521 cttccggtcc gggcggtcaa ggcgtaaata cgactgattc ggcggtgcgg ttgactcatt
    29581 tacccagcgg tttagtggta acttgccaaa gtgaacgttc gcaacatcaa aataaagttt
    29641 tggctttggc ggttttacaa tccaagttgg cccagcagca agccgaaaaa ttgaatcagc
    29701 aaaagcaaac acttaaaggt caacaaatta aagctgattg gggtttgcaa atcagatcgt
    29761 attttttata tggcagccaa attattaaag atcatcgcac cgcagtcagc actagcgatg
    29821 tcgccggggt tttagacggt aaattagagt tatttttaaa ggcttactgg ttatgggatt
    29881 ggcagcggcg tcaagccgat ccgtctgctt aaaagataat tgataaaatt atgtccggag
    29941 ggggacaatt ctttccggct agccgttatt ggctggcttt tattttcggt attgcgttgg
    30001 tcgggttgtg gccgagtcat tggccagtgg cttattttta tggtggttta ttggggattg
    30061 ctttggctgg tttgaccggt aagcggaagc agtcagtttt agtattggct ttatgtttag
    30121 caattggttt ttggcgggcc gacactgcct tggtatcggc cgttacaaca gcatcatctt
    30181 taagttctag cggcgatatg attattaagg ggcgattggt cagccgaccg cagcctcgct
    30241 ctagcggtgg atcgcagatg attattaagg atattaatta ttatagccat tccggctggc
    30301 gcacttggcc cgggcgagtc ctggtttatg gtcctgaaag ctggttaagc ggagctcagg
    30361 ctggcaaaag ctggcaattt agcggccgtt tagccgcgtg gccagccggt ggtgttttca
    30421 gttatcgtta ttatctgctt aatcgcagcg ctcgggctat cttgcaggcc gaccagcggc
    30481 agctggtggc tagccagcct caaaactggt ggttttattt ggttaatggc caagagcgct
    30541 tatctcaggc gttagccaat agtttgccag aaccggcggc tagtttggct ggtcccttag
    30601 tttggggtgg cgcaggtcaa atatcggctg agtggcgtca acggctggcg attaccggtt
    30661 taagccatat taccgccgtg tccggtttta atattagctt gttgcttgtt ttatttattg
    30721 ataattttat tattctgggc tggccgcgcc gctgggccac aattagcgcc agtttggtta
    30781 ttgctattta tgttttagtt atcggggcgc cagcttcagc cgtgcgggcc ggtctttttg
    30841 gtttgttatt ggtctggggc cgttattgcg gtcgcctggg ccggagcggc catctgcttt
    30901 taattagcgc ggcggttatg ttggccatta atccttttta tttttggggt gatttgggtt
    30961 ttagtttgtc atttgccgcg gttttcggcc tgctctattg gactgaattg tggcaaaaat
    31021 ggttacggcg gataccgtta ttgcggctgc cactgattag tgaaaattta gcagtcactc
    31081 tggcggctca gatgtggact tggccgttaa ttttttggca attcggtcaa atttccatga
    31141 tagcgccatt ggctaatttg ttggtggttt gggctttacc atggttgacc gtctttctct
    31201 tactggctag tcccttggct tggctctggc cagccggcgg ggtttttatt tttgccccag
    31261 ccggttggtt gctaaagatt ttgttagcca taattgctaa tttagctaat tggccggcgg
    31321 ccggttttat agtagagtgg ccgagtggct ggcggattgg gatcattata atttattatg
    31381 gattaactgt ggttttgctt agacgaagaa aatgaagaga ttagtttttc accttgcttt
    31441 tccaaggtct ttttgttatg ctaacagcag tttctcttga tttttatgac tgaattagct
    31501 aaaacctacg aaccgcaaaa ttacgaagcg gcgatttatc agcgttggtt ggatagcggt
    31561 ttatttaatc cagataattt gccactgccg gctgaagcgc ccaactatac tattattttg
    31621 ccgccaccca atattactga caagctgcat ttaggccatg ccgctatgtt ggccattgaa
    31681 gatttattga ttcgctatca tcgagcccgc ggctatcgga cattatggtt gcccggcact
    31741 gatcatgccg ccatcgctac ccagaatgtt gttgagaaat tactttatca gaccgaaggc
    31801 aagactcgcc atgattatgg tcgggaagag tttttgcggc gcgtttggga gtttgttggg
    31861 ccaactagag aaaccatttt gcatcaaatt agaagaatgg gcgcctcgct ggattggtcg
    31921 cggatgactt ttactatgga tgagccgcga caaaaggcag tgcgggtaat gtttaaacag
    31981 atgtatgacg agggggcgat ttaccggggc gaacgggtgg ttaattggtg tccgcgttgt
    32041 caaagcacct tgtccgacga tgaggttgac tacaaagaac agccggccga attatatact
    32101 tttaaatatt cagctgattt tccaatcgct attgccacca ctcggccgga aaccaaatta
    32161 ggcgatacag ctgtagcggt caatccgcaa gataagcgtt atcagcaata tatcggccaa
    32221 acctaccagg ttgatttttg cggccagcca ttgacgatta agattattgc tgatcggtcg
    32281 gtagatatgg cttttggtac tggcgcggtc ggtgtgacgc cggctcattc catgactgat
    32341 tggcaattca aagaaaaaca cgacttagcc gtggttaaag ttattactcc tgatggttta
    32401 atggcggaca atcttggtcg ttttagcggc ttgccggttg cagaagctcg tcaattagtg
    32461 gttgctgagc taggccagcg ccagctgtta attcaacaga caactattac caataattta
    32521 tctatctgtt atcgctgcgg caccgctatt gagcctttgc catcgaaaca atggtttatt
    32581 ggtgttgatc gtcccttgga ccgtttgggc ggccggtcgt taaaacaagc cgccatggca
    32641 gcggtggaac agggtcaaac caaatttgtg cccgagcggt ttcaaaaacg ctacttggat
    32701 tggatggcta atttacatga ttggtgtatt tctcggcaga tttggtttgg ccaccagatt
    32761 ccggtctggt atcgtggtga agaaataatg gtcagcgata ctgcccctca tggtggcgat
    32821 tggcagcagg atccggatac tttagatact tggttttcat cgggcatgtg gactttttcc
    32881 actttaggct ggccggataa ttttgttgac ggtcaaaaga ttggcgattt agcccgattt
    32941 catccgactc aagttttaga gaccggttat gacatcttga ctctgtgggt gtcgcgcatg
    33001 atgatgatga ctctttttgc tttgggtgaa gtgccattta ccgatgttta tatgcacggc
    33061 ttggttttgg atgaacatgg caagaaaatg agcaagtcca gaggtaacgg ccttgatccg
    33121 ttagatatga ttgagcttta tggtactgat gccgttcgct tatcgttatt gatcggtatt
    33181 actccgggta atgattgtcg cctggggacc gctaaaattg ccagttatcg taattttgtc
    33241 aataagcttt ggaatattgg tcgttatatt ttgtcagcac tgcctgataa aacagttttt
    33301 gtccataata acatagactt aacacaaaca acttgggctg accgctggat tctgagccgt
    33361 tttgataaat tggttaagac ggttggccaa cgcattagcc gctatgaatt ttcattagct
    33421 ggtgaagctt tgcgcgattt tacttggaat gatttagctg attggtattt ggaaatcagc
    33481 aagatggagc cgactagtca gaaagctaat attttgggct atatttttaa aaatctattg
    33541 attctgtggc agccgtttat tccttttgtg actgaagtcc tctggcaaca gctcgggatt
    33601 gatcagccgc tattaattgc tcaatggcca gaacagttga gcggtgattg ggcagatgcc
    33661 gaatctttta ccatagttca gcaattaatt actgaaattc gcaatttacg cagccatcaa
    33721 caaatcgctc ccaatcaaaa cttatccgcc ttgattatcg ccggacaaca ggccgaagcc
    33781 ttaaacagtc agatagattt attaaaaaaa ttgcgcaccg gtttagaaca agtggagatt
    33841 gtagctactg gctcagctcc ggccgattct ttggctggcg tttgcggtca gattagtttt
    33901 tatttatttg gtcaaattga ccaaactggc gctcaagcta agattgctaa gaaaattgtt
    33961 gagctgacaa cggccattga gcagttatct atccgtttag ctaatcctga atttatcgct
    34021 aaggcgccga aaaaaattat tttagccgaa caaaataaat tagctgattg gcaagccgaa
    34081 ttgacggctc taaatcagca aataactaaa taatatgtgg tttttgaatc tattattatc
    34141 attattagcc attgtaatta cttggttgct agccagttgg tcggctagtt ttagcaagcg
    34201 ttgggccaat cgtcataagg acaataagat taatccaacc atgcatttga ttgttcagcg
    34261 ggcaattcaa acggttatct atattttagg cctattgact atcggccaga tttggcatat
    34321 taatatcgga ccgctctggg ctgggttagg tgttgccggc gtggctatcg ctttgggttt
    34381 acaagaaact ttatccaatc tttttgctgc cttttttgtt attattgata aaactattcg
    34441 ggttggcgct tatttgaaat tggacgatgg cacggtagcc aaagttgagg atatctcctg
    34501 gcgctctact cggttgaagg cgaccagtgg cgagcgaatt attatgccta atcgtttatt
    34561 tgctaatcag aaggttattg gtcgcagtca gactgataaa aactggatgg tgactattga
    34621 agctatcgcc ggcccgggca gccaattgac cgaggttgaa caaggtgcgt tggcggctgg
    34681 ccagatagtg gctaaaaaat atccggccaa aacggctacg gttaagtttt attttgacga
    34741 attgacggcc gctggcttaa aatttaagtt agtcattagt attgacagcg ctgccaatga
    34801 aacagctgcc cgtcatgaat tattaaagaa tttgattatt gagctggcca agcggtcttg
    34861 tccgttagtt ggccgttaat tatatgatta agactgatgc taaattgatt gatgaattac
    34921 tggaacgtgg agtaaatgaa gtaattatta aagatcagct gcggcaaaaa cttctaagcg
    34981 gtcaaaaatt acggattaag ctaggcactg atccaaccag tcccaacctg catttaggtc
    35041 gttctattcc tttgcttaaa ctgcgcgatt tgcagcaatt aggccatcag attgttttaa
    35101 tagttggtga ttttaccggc gtcattggcg acacttccga taaggatagc gagcggccga
    35161 tgctatcgcc cgaaaagatt caggcgaata tggctcagta tttaaagcaa gccggtcaat
    35221 taattgattt agatagctgt gaatttcatt ataatagcga gtggttaggc caactaactt
    35281 ataatcagat tggcgagcaa gccgatcagt tttcggttgc tgaatttatt gctcgggaca
    35341 atatccgccg tcgtctagaa gctggttcgc gggtgtcggt gcgtgagatg ctttatccgt
    35401 tgatgcaggg ctatgacagc gttatggtca aggccgatgt tgagctgggc gggactgatc
    35461 aacgttttaa tttgttggct ggccgtaaac tgcaagaaca ttttggtcag ccaccgcaaa
    35521 atattatcat gaccgaattg gctgagggca ctgatggtcg caagatgagt tccagctggg
    35581 ggaatactat taattttacc gatgaaccgc agaccatgta tgctaaagtc atgggcatca
    35641 gcgatgattt aattatcaaa tattttattt taatgacacg ctgccccttg agccaaatca
    35701 cagaatacga acaagctatc caaggcggcg ctaacccgcg cgatgcgaaa atggcgctag
    35761 ccaaagaaat tgtcaatatt tatcatggcc aagtcgcggc tgagcaagct gaagcctatt
    35821 ttattaaagt tgttcagcag aagttattac cggaggatat tgccaccatt gctttaacta
    35881 agagcgactg gccgattatt gatttattat tagacattaa ggcggccacc agccgtggag
    35941 aggctcggcg cttaattgag cagggcggtg ttaaattagc cgagcaaacg attactgata
    36001 gccaattatt agttaaatta agcgctaagc caattttatt gcaaaagggt aagaaacaat
    36061 attggtattt ggttgctgaa taatatgaac ggttattatt atcaggatat aacttcaaaa
    36121 ttagctgact tgattaatca gcaattacca gaaccgctgg ttcaggcgga tgatattgtt
    36181 attccgccgc aagccgatat gggcgattta agttggccct gttttcaggc ggctaaacag
    36241 ctggggattg ctccaggcgt tttggcgcaa gatttggttg ctaaattggc aactggtaat
    36301 aatccgatga tttccggctg gcaagccgct ggtccatatt taaatattaa tttatcgcca
    36361 caattattag ctgagaaagt tttaaccgag attaatcaag aaaaagaaaa ctatggtcgt
    36421 tcggcagagg gtcagtcgaa gcgaattatg attgaatatt ccaacgccaa tacccacaaa
    36481 gagtatcata tcggtcatct gcgcaatctg ttttttggcg acagtgtcca gcgaatctta
    36541 aattttgtcg gttatcaggc tattcccgta tcctatttaa atgattttgg ggttcatgtc
    36601 gccaaggttt tgtggaaact gagtcaacag ccagctgaac agataattcc ggataatcgc
    36661 ggttattact tggggcagct ctatgctcaa gcgagtcagg aattacccaa tgatccagtc
    36721 ggtcagggct tggtcggggc gattatgaaa cgcttggaag ccaaacagca gccggaatat
    36781 ggccggtggc aagaaactcg ccaatggagc gttgatcagt tcaatgatat ttaccgtcag
    36841 ctggatattc attttgacca gaccttcttt gaaagcgatt atttggatga cggccgtcaa
    36901 ttagtcgaga aaatgctagc tgccggccaa ttagaaaaaa gtcaaggcgc tgttttggct
    36961 aatttggaaa atgaaaaact gggcgtttta ttatttttac gcagtgatgg caccactctt
    37021 tatccggtgg ctgatttgcc gctggctcag gacaaattca agcaatacca attagccgaa
    37081 tcatggtatg tgattgatga gcggcagagt ctgtattttc agcaattagc cgctgtttta
    37141 cgtcgtttag gtattaacca accgattcgt catttgtctt acagttttgt taaattacca
    37201 accggcatga tggcttcacg ccaaggcaat attattactt atgaacagct caagcaggat
    37261 ttattgaata aagaattggc tgagattaag cagcggcatg ccgattggtc tgaagagcaa
    37321 tgtcagcagg ttgcttggca agtagtggtt ggcgcgttaa aatttgaaat ggttaaagtt
    37381 ggcccggata agccgattac ttttgatctt aatcaagctc tgagttttga cggctatacg
    37441 gctgcctata ttcaatatgc agctgttcgc ttagcggcta tccagcggca agccgcttgg
    37501 ccggaatcgt tggttgttcc catcgcgccc attgaggaaa aagccatttt attgacagcg
    37561 gcccagttta atcaggttat tagccaagca gctcaccagg ctgatccgtc aatattggct
    37621 aaatatttat ttaatttagc tcggcaattt aatgattatt atcagcgcgt gcggattatc
    37681 ggttcccagg aacaagcttt ccgtttgcat ctagtagcgg ccatccgcca agttttagat
    37741 aatggtctta ggcttttggg tatccaggcg ccagacctaa tgtaaattta tgttacccaa
    37801 tgaattatta gaacaattac gttccgagct agcgattgtc actgacgaat ccgggctagg
    37861 cgtagtgtgg cagaaatatc ttggcaaaca gggtttaatc aaaaaacagg ttaaagcgct
    37921 gtcagcctta acgattgaag aaaaacgcca acaaggaccg gcctggcaag cggctcagca
    37981 gaccgctcag caattatttg atgatcactc agctgaattg gccgcggtta gtcgacaagc
    38041 gacttgtcag gcacaagctc agactatcgg tttaaaaccg gttaagctcg gccatctgca
    38101 tccgattact cagaccattc gtcagcttaa tgatttattt attaaaatgg gctatagcgt
    38161 tatggaggat cgagagattg aaactgacga gttttgtttt cagcgcttaa atgtcccgat
    38221 ggatcatccg gcccgcgaca tgcaagacac tttttatcta caagagccga atattttatt
    38281 acggacccag acctcatcgg ttgaggcgcg gctgctggct aattatcagc cgcctttcaa
    38341 ggtggtttgt ccggggcgcg tttatcgcaa tgaaactgtt aacaagagta accattttat
    38401 ttttcatcac taccaagggg tggttgttat gaacaaaact agcttagctg atttgtttgg
    38461 cacttttacg gctttatttc agtcagttta tggtccggag gtgaaaattc gtttccgcaa
    38521 taaatattat cctgaagttg agccgggtgt tgggccggat atgcaatgtt ttaattgcca
    38581 aggcgctggc tgtccaattt gcaaaggtgt tggctggatt gaaatgggtg gcgccggctt
    38641 gattcatcct aaagttttaa ctatggccgg gctagatccg gagcagtggc aaggctttgc
    38701 ttttggtctc ggccttgatc gctgggcaat ggctcgacac aatattacag atgtccgtac
    38761 cctattgggc ggtaatttag gttataaacc ctacagccat gaaaatactt tatagtcaaa
    38821 ttaaacaatt agtgcccgga ttagtggcta cgccggcgga agttggcgaa gccttgacga
    38881 tgactgggct gatgcaagaa agcttaactg cggtcaacta caatggccaa gccgatacct
    38941 taattggttt agaagttcgt caaaataggg ctgattgttt ttcggttcta ggtatcgccc
    39001 gcgaagtggc ggcttatttc aatttggcat taaaattgcc cgaccttaaa ccatggtcgc
    39061 cgactaattt tattttgcca attcggattg acactgatca agttcgccgt gttcgggcag
    39121 ttaagttatc aggagccgtt gccaagccat caccgcaatg gttagttgat taccttagtc
    39181 tatatgaaat taagactgtt aatctagccg ttgatttatc caattatgtc atgatactga
    39241 ccggctatgc ttcgcattta attgattatg atcgactaga tggcgatgtt gtttggtcag
    39301 ttaacgatcg agctgttaaa gttacgactt tagatggcag tcagattgat ttaactggcg
    39361 gcgaactgtt aattagtgat cgtcgagaaa ttattgcttt agccggtatt gttggtactg
    39421 attacgccgc catcaagccg gaaacgacta atattttact agagatggcg gcttataatc
    39481 ccagtttagt gcggcaaaat tcgcgccgtt tgcatattgt caccgaggct agtaataagt
    39541 tagaaaaaga tttgagttta gctaacttag attacgcctt tgatttgtta gttaatttat
    39601 tggttgacca aattggtgtt caggtcgcca gccaattatt tgaatattat cctcaaccga
    39661 ctcaaccgct ggttattaaa tttgatccgc aattagcggc tcagatgggc ggtgttgaga
    39721 ttagttcaca gcaaagtcag caaatattac agcaacttgg ttttgttatt gacaacaaaa
    39781 ataatccatg gctagtaacc gtaccagatt ggcggactga cttggagatg gctcaagatt
    39841 tggtagaaga agttttacgg ttatatcgtt ttgataaaat tccagctatt ttacccgccc
    39901 tgaccgttac tccagaaatt actttgccgt tgaccaaagc taaagaacag tggcgagatt
    39961 ggctagtggc tcagggctgg gatgaggttt tgtcgtggac gatggttact agagctaaca
    40021 atcaagaggc caattatttg ccgtggtcaa tagccagcac ccagaatgcc atcaatgaag
    40081 attatcctga tttgcggcag acaattctgg ctagtttaat tcaacaagcg cgtgaatatc
    40141 ttaaaaaaca ggtgccggat ttacaaattt ttgaaattgg taaagttttt ggtcaagtcg
    40201 gtgatgacta tcaagaagct gaacaccttg gttggctatt agccggtcaa gatacggtgg
    40261 tggagcaatt gcgtcagaca gttgccggtt ttttgggttg ggctggagcc aagcagataa
    40321 cttggtctca attagataat attccgccag ccggcaatcc ttacgccgct tggcagattc
    40381 agctggccgg tcagtctctg ggtattttat gtcaactgat tgatgattat aacggacaaa
    40441 ctatggttgc cgctgagatt gatgctaatc aattaatcaa tcatttaatt aatgtcggca
    40501 attttccagc caccgtggag ttaactcaga aattaattag tttggatgcc aatttagaat
    40561 tagacaatcg tcaagcgttg gacgctgaac tagccgatat cacggctaag gtcggttatg
    40621 aaggtttgtg gagcttatca gtagtggatg aatttgctct gcctaacggc cgttatcgct
    40681 atacggtgcg ggcggtctat aaggaattag acgatcagtc ggctaaaaaa ttacatttac
    40741 aagtttttgg tttaaattag ccaatattaa tttatgggtt tattacgatt agcgcggcgg
    40801 attattgatt gggcctttaa acagttctat tcaccacaat ttgtgcgtta ttttattgtt
    40861 ggctgcagcg cggtgttgct agatatggca acgctttatt ggctgaaaac aattatcggc
    40921 ttgactgctg tttggtcaat tgttattaat caattggcta tgtgttctta tgttttttta
    40981 ctaaacaaat attgggtctt tgcttcatca ggcgcttttg cccgtcaggc ggttcgttat
    41041 ttttcattgg cagccgctaa ctatctgatt gccatttttt ggatgtggct atttaatgaa
    41101 cagtttggtt ttaattatct attagtccga ttagttaata ttgccttatc aaccgtttgg
    41161 aattttttgc tttatcggtt ttttgtttat gttaaaaaat gattaataat agctagtcca
    41221 attttgattg acagctagtc ataggcgtgc tatgattttg gcataatgtt aggtagttta
    41281 gcctaatctt aagtaaagta aaaagtatgg agcttcatga caaagagttt ttagagtatg
    41341 ttgtcaaggc catcgtcggc aatccggaag atgtttccgt tgaccgcact gttgacgaaa
    41401 tgggcgtcct gttgacctta aaaattaatc cggccgacat gggctatgtt attggtcgtc
    41461 gcggccagac tgcgcaggct atccgcactt tattaaagat tgtcggcgcc aaaaatagcg
    41521 cccgggttaa cctgaagatt tatgatccga atggcggcca gcgtccgcat cgggagtttc
    41581 atcaggaaaa accagtcgag gaaaagattg acacttcttc agtagaagat ttagccatct
    41641 agtatctgta ctcataacaa aaagaccaga ataattctgg tctttttgtt ttatgctata
    41701 atagtgcata tgttattcca aattattaca gttttgcccg aggcttttgg ttatttagag
    41761 caatcaatta ttggtcgcgg ccaacagcag ggccattttc aagtaaaaat tcatgatttg
    41821 cgaaattggt cgcttaatca acagcggcga gttgatgata cgccctatgg cggtggtccg
    41881 ggtatggtga tgcaagttga gccgattgct caagtgctga aagctattaa aactaaacat
    41941 aaacacaaaa aaatagttct gctatcggct aaaggtaaaa catggaccca agcgcgagct
    42001 cgtcagtata gtaaattaga tgaggtggtg ctgatttgcg gtcgctatga aggggtggat
    42061 gaacgagtta ataatttaat tgatgaagaa atctctatcg gtaattatgt cctaaccggc
    42121 ggcgagctgg gggcgatgat tatacttgat tccatcgctc gccttttgcc cggcgtttta
    42181 ggtaatgccg atagtctgct ccaagaatct catagtcggg ttggtcaatt agaatatccg
    42241 cagtatacta agcctgaagt tgttaattta gctggcatag attatgctgt gcccgagatt
    42301 ttactgtcgg gcaatcacca agagattgct aaatggcgtc aggcgcagat gaaaagaata
    42361 aaaaaataga gcagatgccg tatggcagag ctgctctttg tttctaaatt gttgttaggt
    42421 ggccagcata tcatgcaggc cactgcctag tattggtagg tttttgggga gttgagcaat
    42481 ccgctcgtct cccaattcct ggcgcataaa gcggatttgc gcccgagtgt gttctcgggc
    42541 caggttgagc ccttttagcc aggcagtttt taggggctcg tccaggttca taaggttata
    42601 catgacgacc ttagcttcgt tgaggccgtc aaaataggtt actttttcca ccagccattt
    42661 ttccacggcc agttggtagc tttcaccacc ggtgttgccg gtgatgaatc tattataaat
    42721 ctcattaatt atatccatca gccaaggggc gtaagttgtt aacacccggc ggacatcgcc
    42781 gtagtgataa cggttcagct gactatgtgt cagttggttg ttagccaagg cggtaaaatg
    42841 ccgcagggct gatccggttg ggaattgccc gtcgacatat ttgatcaccc ctagctgatt
    42901 aatgaagacg tagttgatga cgtcccagat attaatcaaa tatcggccta cgaagaagcc
    42961 actgacacag tcagcctggt caatggcctt ggtgacatcg ctagccacca ggacctctcc
    43021 gttgggagct agtgtcggat aaggagccag cttatattct tgaccaccga ctaacactgt
    43081 tctgtcctca gtgtagaagg tgtgagcggc ggcactaaag acggccatgc cggccgcata
    43141 aggatttttg gccgtgaagc cgatatgtct aaggcatagc cttagtaaat atccgccaat
    43201 atcggcatgg ccgatattgt ttttttcggc acagttatag gtgccgaccg ctaggtcatg
    43261 ggtcagtcca gcgattgccg ccgccaaggc atcgtcggga tgcattgggg tgccgggcag
    43321 cataattagg gtgtttaaaa catccctaat aatatgtcca cggccatgag cggccgtgaa
    43381 gtttgaggaa aacccacaat ccttatacca gtttagtatt tctggtagcc attgcttgaa
    43441 atcttgcaat gggccttgag aaactgggtt ggttggtttt ctattacaaa gtcggggaag
    43501 atcactttgg atcttctgga caaactggat gacctccagt ttgaactggt catcgctaaa
    43561 aaagatttgt ttcatttttt tctccttttt tcaattgttt atgtgcgttt atgtgctttg
    43621 tttagtatta accagaccca ccattaaaac acacaggatt atagttgtca attttaaata
    43681 gcctctctag atataccaga aaggctattt tgcctgtacc tcgggagagg ctcgaactct
    43741 catgagatca ctcccacacg attttgagtc gtgcgcgtct accagttccg ccaccgaggc
    43801 aacgaaagct attataggtt agctgataaa aattgtcaaa aaattggcaa tcagttatac
    43861 taaagcagta atcatagcta gccatcatga ctaaaataat cgctattgtc aatcagaagg
    43921 gcggtgttgg taaaacaacg accgctgtta atttggcggc ctatttagct caggccaaac
    43981 gccgtgtact actaattgat attgatccgc aggctaatgc cacctctggc tccggtctga
    44041 tttcagctag ccaagctacc ggtctttatg aagctttggt tggccagcaa actatggctg
    44101 atattattat tgattcgccg gccggttatc gggtcgcgcc agccaccatc aacttagctg
    44161 gcgccggtgt tgaattggtt aatctgccgg agcgagaatt tcgtttggcc aaagtcgtgg
    44221 acgaagtgcg gactgatttt gattatatta ttattgacgg tccgccgtca ttgggcttgt
    44281 taaccatcaa cagcttggtc gctgccagtc atgtgttaat tccggttcaa gctgaatatt
    44341 atgctctgga aggattaaaa caattattag ataccatcaa tttaatccaa actcatatta
    44401 agcccaattt gggaattttg ggagcagtga taacgatgta tgatcagcgt aatcgtctgg
    44461 cccgggccgt agtaaaagaa ttggagcagt ttttccctaa tcgggtcttt aaaagtatta
    44521 taccgcggaa tgttcgttta gctgaagccc ctagttatgg tcaaacaatt gttgaatatg
    44581 atcgtcattc cagtggtagt caagcttatc aggccttggc caaagaaatt ttagctttag
    44641 aataatttta ttataacgat atgccagcag gtttaggcaa gggtttgggt tcattgttag
    44701 cggctaatcc attagttgaa ccgatggaca gtgaacgcat cgtcaattta gcgccggatt
    44761 tgattcaagt taatcgccag cagccgcggc acgattttaa tcaagaaagt ttgcaagagc
    44821 tagctgattc tattaaagaa tgcggtattt tgcagccgtt gattgtggct aaaacaacgg
    44881 ccggctatga attgattgcc ggcgaacggc gtctgcgagc tgccaaatta atcggtttaa
    44941 aaacagtgcc agcgatggtc cgctcggttg atgaacaaca gaaattagcc ttggctttag
    45001 tggaaaatat tcagcgagag aatttaagtc cgttagaaga ggcgacggct tatcgacggt
    45061 tattagacga atttcaatta actcaagacg agttggctag ccgagtgggt aaaccgcgtt
    45121 caactattac taatttactg cgtttactta atttgccggt ttcggcccaa caagcgctgc
    45181 gttcaggcca aattaattta ggccaggcga aagttttggc gggagttgat ccggctaagc
    45241 aagatcaggc tttgcgtgaa atgaccgcta aaccgattac ggtggctgac tccgttaaag
    45301 tagcgcgcat ttttaattct cggactcaat ctaagacaac accgacagcg aaggatgtat
    45361 ataatgataa aggtaaagaa ttcttattac gggaattttt tcaaactaag gttaaaatta
    45421 cgcgccaagc ccgtggtggc caaataatta ttagctttga tagcgatgac caattgtcag
    45481 cgcttatgaa caagattcgc cgctaactgt tggctttcca gcgaccatta ttaataatga
    45541 ttaagttgtt ttttattata aatcagaagc tcgataaaga taataaggaa cgctaccgac
    45601 cggtcgcagt tttcgttgtg gttggtagcc agctaaaggc cagtcgccga ttacaatttt
    45661 agtggccggt gatagctgac tcttaaattt tttaattaat tggggataga ttttaggcag
    45721 taaaaagaca tagacagcat cagcatcatc cagtcggtaa tgccaaagat tagcccagcg
    45781 gatggtaaca ttagcttgcc gtctcgtctt ccaagcggcc gccagggcca gtagcggcga
    45841 tatctcaagg ccgattaagc ggctgtctgg attttttcgg gctaaataag ccaataaatg
    45901 gccatggccg cagccgattt cataaaatat ttgtccaggc tgtaaatcta ataattgatt
    45961 gattgctgcc caatcgcgtt gtctggtggg cacccacggt gccgctgacc aagctccggt
    46021 ggccaaactc aataatatta agctgctgac aatggttagc ccggttaaga gatccatatt
    46081 agtggtgatt aaaattaatg gcgactatct ctggataggc gttaattcgt tggttaaatg
    46141 agctattacc caggccgcgg ttgattatta atcggctatt gccaactggt aaattgccat
    46201 aatcataatc tggccatagt ttttggttgg gtacatagat ggcgcccagc agcggcagtc
    46261 taatttggcc gccatgagta tgtccggcca aaatggcaat aggctcaata cttagatatt
    46321 ctggccataa ttcggggcga tgagccaaga caataatcgg ttgattgact ttattttgat
    46381 taattatctg gcctaatttt tggctggtga ttttggcgac gactgacgct ggtaagggtc
    46441 cgccaagacc agtcggatca tccaagccga ctaaagtaat agtttggttt tggctttgga
    46501 tagttagagc ctgattgcgc accactatag ctccggcagc agtggctcgt tctgcgagtt
    46561 caatgtagtc attattataa aaatcatggt tgcccaaaat aaggtaaacc ggagctaagt
    46621 tttttagccg attgattaaa aataggttgc ttggaatatt agtttggcgg ctatcaaaag
    46681 tatcaccggt taaaacgatt aagtcagggg ctaatttttc aacggccgcg ataatctgtc
    46741 ggttgcccgg gccactgata gtctggttat gcaaatcgga taattggaca attcgccaac
    46801 cggttaattc ggccggccag cgttggtcgt tgatattata ttgtttgatg gttagatggc
    46861 gattttggta atttaaagtc agcgcgatcg ttagagctaa taaccaaaaa aacaaccact
    46921 ttaaccagac gggtaatggg ataatcatat tgtttctctg gtggcatctt agcacaacta
    46981 tgttataatt aaaaatatgt taattaaact taatcggccg gttagatggt ggtttaagca
    47041 aactaaagtc gcgcgagttt tattgacagc ttcgcttgtt ggttggctgg tattgctcta
    47101 tttagctagc cggccatttg gtgttgctgt ttatagttta agcagccagt ctggtaatta
    47161 ttttatccat aaattcggcc caactgaacg gtggtcaacc tctagtcccg atttaataat
    47221 gaccggccag ccaggttatt tttttctgcg gccgactcgg ccatttaatc aagctgagat
    47281 cactgttact tggcaagatc gtcagccgga tacctattta gaggcgggga ttttgatgga
    47341 tcgggctaat tggcagtatc aatcacagcc gttaaatcat cccggcttaa ataagctaga
    47401 ttggccgttg gtaaccgccg gccaccatcg tttgtggcaa aaaaccccag tttatcaaac
    47461 ctggtcagag tttattgaac gtttgcctaa ttttagtcag ctagctgttt ataattggtc
    47521 gcctagctca actaaatcaa tcaatttgac cggttatcgc tctcatcaag tctggcagca
    47581 gcttcctggc cagtggcgcg gtcttcagca gatagttact tatttggccg atgatgaaca
    47641 attggcttgg cggataacgg taactagcga ttctttacaa gaattgactg ctgacgaacg
    47701 gttagttgaa gttaatatta ctaatagcgt taatcagcgc ctgtggtctg agcaacgtcg
    47761 cttggacgat aatcaattgg agtggttgat tacggccggt ccgtggtcag ccggcgctta
    47821 tcgtttagaa attaaagcca gtcggcagtt attactgaag ttagctactc aacagacggt
    47881 ttttggttgg cagggccaag tttggccggt ggcttcagct gattatcact ggcgtttttg
    47941 gactgatgct ccggccatta tcgcgcaaac tatccagccg gccagccggc aaataatttt
    48001 agttaatcag cggccattaa atttagctga tacctatcag caatactcca ttgaaacatt
    48061 agcgacttcc agtgaaatca gcttatccgg cggggatgtt cagctagctg gcagtggtat
    48121 tttttcttta caaccgacta tctttaatcc tcagtggcga cgcgctggtc ggttatccgt
    48181 tgatcagtta gccgggtttg attttatcgc ggccgattat caaccgccgg tttgtcgcca
    48241 gcatttatgt accgccaccg ctaaattatc tttggccgga gccgattggc aatccggcga
    48301 tggctaccaa atcatgctgg cggccgataa tagtcattta tcaactgtca gtccgctggt
    48361 tattcaagag attagtgtta aattacgcgg cgctagcctt tggcagtttc ttcgatcatt
    48421 gccgcgtcgc ttagtaaaca agttgcaata atctatgagt attaggcgac aaattaagat
    48481 taagcaatta aatgatgatt tgtatttatg gctaaccgtc actattgctg ttttaatatt
    48541 ttccgaggcg gtttggccgg gcctggttag cgcttattta aacctatcat tctggttgct
    48601 tttttggtcg gtcagtgcta tcctgaaggt ggtttttgtt aataaataat attttttctc
    48661 taaatttatg attcaagatg tcatctttaa aaagttgacc gtctatgaag atgcccgcgg
    48721 ctggctagct gagatctacc gcaatgacga agaccattat caaccagcta tgggatatat
    48781 cagtgttact cgtcccggcg tggctcgtgg tccgcacgaa cataaatatc aggctgactg
    48841 ttttgtcttt gttggtcctg gcgattttga attatatctt tgggatcgtc gtgaaaatag
    48901 ttcgaccaat ggccaacata ttaaagaagt ggtgggccaa agtaatccga ctatggtgat
    48961 tgtgccggcc ggcgtagttc atggctacaa ggcgattggt aatcaagatg cctggtcggt
    49021 caatttgcca gataaactat atcgcggcgt tgataaaact gaagaggttg atgaaattcg
    49081 ctgggagcaa aatcctgatt ctccctataa aatcatttaa ttattagttt agagttatgc
    49141 ggctattagt aaccggtggg gccggtttta tcggttccaa ttttattcac tattggctcc
    49201 aaaaacaccc cgaagatcaa ctgattaatc ttgatctttt aacctatgcc ggtaatttag
    49261 ccaacttgga ggatattgcc aatcaggcta attatcgttt cgttcagggc gatattgctg
    49321 attacgcagt ggtggatagt ttgatgggtc aagtggattg cgtgattaat ttcgcggccg
    49381 aaactcatgt cgatcgatca attggtgata gtttgtcttt tgttaaaact aatattcagg
    49441 gcacagccaa tctactagaa gccgctcggc gtcatggtgg cgttcgtttt caccaaattt
    49501 ctaccgacga agtttacggt gacttaactc gccacgatcc gaaatttgat gaaacatcgc
    49561 cttatcgtcc cagcagtcct tatagcgctt ccaaagcggc ggctgatcat ctggtgaggg
    49621 cctatttccg aacctataag ctaccggtga ccatttctaa ttgttctaat aattacggcc
    49681 cctatcagtt tcccgaaaaa gttattcctt tgtttgtaac taatttaatt gatgatttaa
    49741 aggtgccgct ctatggcaat ggcggcaata ttcgcgattg gatccacgtt gatgaccaca
    49801 acgccggtat tgaagctatt ttacagcgcg gccaaatcgg tgaaacttat tgcttgggtg
    49861 gcgatcatga aattaccaat ttagaattga ccaagcaaat tatccaatta ttaggcaaag
    49921 acgatagtta tattgaacca gttactgatc gtctaggtca tgattggcgc tatgctattg
    49981 attttagtaa agcgactcgc gatttgggtt ggcgaccggc aatacctttt tcaacagggc
    50041 taaagcagac tgttcaatgg taccaagatc atccggagtg gtggcgtccg ttaaaagaac
    50101 aagtcaagca ataatttatg aaagttttaa ttttaggcgg cttaggtaat ttaggtagtc
    50161 aactagtggt tactggccaa cagcttggtt atgagattat cggctgggat caagaagata
    50221 ttgatttaac cgattggccg gcggttgagg ctaaattagc tggccagcgc tttgatgtcg
    50281 ttattaatac cgttgcctat aatgcggttg atgcctgcga aagtcagccc ggtcaggatt
    50341 tagcttatat tttgaatggc gatttggtgc gacagctggc cggctggtgt cagactaacg
    50401 gcgccatcat ggttcaatat tccagtgatt atgtttttgc tggcgatcaa ttggctggtt
    50461 ataccgaaac tgatcaacca aacccgatta atgaatatgg ccgcagcaaa ttagccggtg
    50521 agcaggcctt aattaaacag ggcgagcagg gtttgcagta ctacctgatt cgcacttcta
    50581 aattattcgg cccacggggc accagtccag cgacgaagag tagttttttt gatattatgc
    50641 ttaagctggc cgccaccgag cccgaactta aaggtgttga tgctgaagag agttgtttta
    50701 cctatacacc ggatttggct caagcgactt ggcaattatt agccgataaa ttcccacaag
    50761 gtatttatca tatagttaat agccagccag ccacctggta tcaggcatta cgcacacttc
    50821 agcaactagc tggattaact gtcccgattc ggccgattac cagcgctgat ttgccgcggc
    50881 cggcggctcg tccgcaacac tcagttttat taatgaccaa attaccgcct ttgcgatcat
    50941 atcagcaggc gttacaagaa tatttaaaca agtctaaata gttttatgcg aggaattatt
    51001 ttgtccggcg gtagtgccac tagattgcgt ccttgtacga aggttacttc taaacaatta
    51061 ttgccgatct ataatcggcc gatgatttat tatcctctta atactttggt caaagccggc
    51121 attaaagaga ttctaattat cgtttcaccg gagcgagccg gcgattatct aaatttattg
    51181 ggcagtggcc gccaattcgg catcaagctg acttatgaag ttcaggataa gccgcgcggc
    51241 ctagctgagg cttttattat tggggaaaat tttattgatg atggtgatgt taccatgatc
    51301 ttaggtgaca atatctttga agatgattta actgacgata tccgcaactt caagagtggc
    51361 gctaagattt ttgccaaaaa agtgcctgat ccagaacgct ttggtgttgt ggaaattgat
    51421 gataaaatgc aggctttgtc aatagtggaa aaaccattgc aaccgaaaag tgatttggca
    51481 attaccggtc tgtatattta cgatcatcgg gttattgagg tggccaaaca agttcagccc
    51541 agcgctcggg gtgaattaga gattgttgat ttgcataatt ggtatttgga acgcaatgaa
    51601 ttagaagtgg cgatgattaa tggcgaatgg attgatgccg gcacttttga tagtttgttg
    51661 agcgctcaaa tattagctcg cgatcgactg caaaacaaat taattattta atattactat
    51721 tattttacta tgcacacccg aattattgcg acggttggtc cgcagtcttt cagttatcca
    51781 gttctaagca aattggttac cagcggcgtt gacattatcc gtttgaattt ttctcacgcc
    51841 aagattgagc aacttaatga tttaaaaaat aatctgcaga gaattaagga agaaaccgat
    51901 tgtcaaacta aaattatgca agacttgcag ggaccgcgta ttcgcatcgg tcgccttgat
    51961 caagaaattg aattggttga tggtgaaacc tatgttttta ctggtggtca ggccagtcta
    52021 gctaatcaag aaattccgat tgacgacccc gatctttatc aggatctcag aattggtgat
    52081 ccaatttttt tagctaatgg tgaattagaa ctgaagattg ttgacattaa agataaaatg
    52141 atttatgccc gagtagagcg gggcggcctg ttgatgtcgc ataagggcat taatctaccg
    52201 aatactaatt taaccagagg cggtttaact aaaaaagata ttgctgatgt tgaggcggcc
    52261 tttgaataca atgttgacta tattgcctta tcttttgttc aaagcgctga cgatttgcga
    52321 agattgcgta agattattgg taatcggccg attggcttaa ttgccaaaat tgaacgagca
    52381 atagctctag aagtaattga tgagattatt aaagaatcag atggtattat ggtagctcgc
    52441 ggcgatttag gtattgagtt gccaatggaa gatttgccaa ttattcaaaa gaatttagtg
    52501 cgtcatgctc attggcatgg caagccggcg attattgcta ctcagatgat gacctctatg
    52561 attgaacatc cgcggccaac cagggctgag gttagcgata ttgccaatgc tgttttagat
    52621 ggtgccgatg ctttaatgtt gtctgacgaa accgcggttg gtctttaccc taatcaggcc
    52681 gttgcggcca tgaaaaaaat tatttgtcgt actgaaagct atcttaataa ccgtaattat
    52741 tttgatactg aaacagcagc tttttattgt cattacgtta attaaatttt atggcacaaa
    52801 gctctcgcgt ctatgttggt tttttgggct atggccaatc aaccgctaaa tatttaccgg
    52861 attttttagc cagtttgcgg cagcagacgt tcactgattt ggcgataata gacggcgaca
    52921 atactccacc cgaccaggtt aatcctaatg agttagctat caaagatttt cctgaagttc
    52981 gctactggcc agccggggct aatcttggtt ttgccaaggg ctataatcga ctattcaact
    53041 tggcaattcg tgagggggct gagtattttt tagtgattaa tccggatgtt gttttagagt
    53101 caacggccat tgagcaattg gttaaatatt tagacgataa tcaggccgtg gctgcggtta
    53161 tgcccaaaat tcggcgctgg gatttcgtta atcataatct aaccatgcaa attgatagtc
    53221 tgggcgtcgg ccttaccaaa cagggggcat ttatagatat tggccaaggt cagcttgatg
    53281 acaatcattt gacaggaccg cagccgatat tcggttttac cggcgccggt gttttaatcc
    53341 gtttaagcgc cctgtcgtcc gttgcctttg ataatggcca atcgattgaa tattttgatg
    53401 aattgatgtt tatgtataag gaagactgcg atttgtctta ccgtttgcgt ttggctggtt
    53461 ggcagattat gttattgcca gaggcgataa tttatcacga tcgcacggcc gccaaggtcg
    53521 gtcaatcagt ttggagtcaa ttacggaatc gtcgtcgtga gagcaagtta atccggcaat
    53581 ggtcgtattt gcatcaatta atgattttct gtaaatattg gtatttgccc aaaaccttat
    53641 ctagtcgttg gcaagaatta ttttatcaaa agaaacgatt agtagcggcc attttgtttg
    53701 agccatttct tttgtctggc tggcgtaagt tgtggcaaca gcggcacttg gtggctgctc
    53761 gccggcgagc tttagttatt aaaatcaagc cattgtcttt agaagactgg cgtcaataag
    53821 cttatgcttg atttagctat tgttaccgtc ccctttaagg ttaaagacaa gttgcgggat
    53881 aatttacgcc aattatttgc tagtcaaggt gatcttaatt ttgcggtttg ggcggttgat
    53941 aataattctg gtgacggtac tgttgagatg cttcaacaag aatttccgca ggtccatctg
    54001 attgccaatc cagacaatcg tggctttgcc aaggccaata accaagcttt gcgggtaata
    54061 agagctcgct atgttttatt gcttaatcct gatatgctag tcgagccgga tactttggtt
    54121 aaaatgttgg cttgggctga tgccaatccg caggcgactg ttatgggttg tcatttggtg
    54181 actgaaaccg gggagactct gctacatgtt cgccgattcc caactttatg ggatcaattg
    54241 gcgatagtac tgaagctacc ccattttttg ccccatattt tagacaaata tctgcggact
    54301 gattttgatt atagtcaggc ggctccggtt gattcaatac gcggcggttt tttttggatt
    54361 cgggccgatg ccaacggtca agtgccactt ttagatgagc gttattttat ttggtttgaa
    54421 gaagtggatt attgccgtca aactaagctg tcaggtggtc aggtctggta tacgccggcg
    54481 gctaattgcc ttgattatgt cggccaaagc tttaagcaag ttaaacgcgg tcgaactcag
    54541 acctattttc gccaaagcat gattaagtat tttgctaaat gggagccgcg ctggcaatct
    54601 tatttactgc gtctagcctg gccgatcggc gctagtttgg ctatcatcgg tgaaaaatta
    54661 aattggcaag gccgcgctaa aacttaaaat aaatttatga ctacctacaa gattgccact
    54721 atcttaatca attaccaaga ttacgccaat aaatatttag ctgattgttg ggctagtcta
    54781 gaaaagctta atttagctac tgatgattgg caaattgatc acactatttt tattgttgat
    54841 aatcagtccg atgacaaaac ttatcaaaca ctgcagacta tggcgccaac agctcgcctg
    54901 attcgcaatg ccaccaatga tggttttgct aagggtaata atgacgccat tgccatcgct
    54961 atggccgagg gttgtgattt tgtctgggtg ttgaatatgg acaccattgt tgagccggac
    55021 agcttgattg agttagtcaa accaatgtta gttaatcaaa aattgggtgc cgcgcaatcc
    55081 ttaatgcgtt tatggccgga tcaagatctg attaacagtt cgggtaatgc tacgcatttt
    55141 ttaggctacg gatattgtct tgattatcgt aagccagtcg cagaacttaa cctagccgct
    55201 attgatggac aagccatagc ttatccatcg ggggctgcag tactttttcg cttatcagct
    55261 ttgcggcaag ttggtttgtt tgatgagact ttttggatgt ataatgaaga tcaagactta
    55321 ggttggcggt tatggctagc tggttgggat tgtcggttgg cggctcattc tcaaatttat
    55381 cataaatatc aatttagtcg tagtagcagt aagtattatt ggcttgatcg taatcggcta
    55441 ttgtctggct ggaaaaacta tcattggtta accattattg ttttattacc agctctttta
    55501 agtagtgatt tgattttagc tattatgtct tggcgcgctg gtcgcctcaa acacaaatta
    55561 aatgcttggt ggtattttat taatccattg cactggccga tgctactcaa gtcgcgctgg
    55621 caatcacaac gtttacgccg gcggcccgat cgacagatta ttaagatctt tagcggtcgt
    55681 attttgtttc aagaggttga tagccaaaca gttcgttggg gtaactggtt tctcaatttt
    55741 tattggcaag tcattaagct aataattatt tggtaatatg gatattagcg tttccatagt
    55801 taattattac agtcaaaagg ttatctatcg ctgtttagag tccttgcgcc aaactaattg
    55861 gcatggcttg gagcatgaaa taataattgt tgataattcc cacgaaactg atctggccgc
    55921 caaaatagcc aaagagtggc cggaagttcg ttatatcgcc aacagcaaca ctggttatgg
    55981 ggctggtaat aatttagctt tgcgtcaggc caccggcaaa tattttttag ttatgaatcc
    56041 cgatacggca gttgctccag atactatcaa acagttatat gattttatgg aggctcatcc
    56101 tcaagttggt atggtcggtc cgcgacagcg caatcttgat ggtagccgtc aggcctcttg
    56161 ttatcgctgg cataccttag caactccatt ttgccgtcgc tggccgtggt tgagtcgata
    56221 tcgttgggtt cgtcgcgagc ttaggcgctt tctcttggaa gatgttgacc tggaacggac
    56281 gatgccggtt gattggttac tcggttcttt tttactgatt agagccgcag cgatgcggca
    56341 ggtcggttat tttgatgagc gttataagtt atattttgaa gacactgatc ttagtcggca
    56401 gatgtggtat cacggttggc aggtggttta ctatcccggt accgaagttt tacacaatca
    56461 tcaacgagcc agcgcccaag ctgcttggta taaggtgtta aataatcgag ttggtcgcca
    56521 gcatacgcta tcttggttta agtatttatt aaaatggggt ctaatagcta agtctaatat
    56581 tcctcgtaac caataatttt actatgcgca agattactaa agctatttta ccggttgccg
    56641 gtttcggcac tcgttttttg ccagctacta aggcacagcc caaagaaatg ttgccggtag
    56701 ttgacaagcc ggcgattcaa tacttggttg aagatgcggt cgccgccggc attgaagaga
    56761 ttatttttgt tactggccgt ggcaaacgag ctattgagga tcattttgat tattcttttg
    56821 aattagataa aactttagtc gaaaaacata agctggactt agttaagcaa gtgcggcgga
    56881 tttccgaaat ggctaaattt tcttatgtcc gccaaccact gccattgggt gacggtcatg
    56941 ctttagcttg tgcggctcat ttagtttatg acgagccggt tttggtgatg tttggcgata
    57001 ctctctatga tgccgacgag ggtccagcta gccaagtttt aaaaactttt gataaatacg
    57061 gcgatccggt tattggtttg tcggcggttg atcgacaaga agtcagcaaa ttcggcgtta
    57121 ttgatggttt agcgttggct gataatgttt atgaaattaa gcaatttatt gagaagccca
    57181 gtccgcaaga ggcgccatcc aatttggtgg ccgtcggcat ctatgtcatt actcctgatg
    57241 ttttacatac tttgactatg atgaaaccgc ggcccaacgg tgagttgcgt ttagctgatg
    57301 cttttgtgca aatgttagcg gctgaccggc cgttatatgg gcaagaacta attggtacct
    57361 ggctagatac cggcgataag cttagcatga ttaaggctaa tattcagctt ggtctgaaaa
    57421 acaaagagat tggcgctgat ttgcgtcgct ttattgaggg gatattgcgt tgattatagg
    57481 gccagagaaa atagtccgct cttggtgccg atccgtccat aaaaatagag ctggtcgact
    57541 tttttattaa ccgctgccgc cccgagctcg tcaagttcgg ctaactgcca gatattgtat
    57601 ccttggcgat cgtctaattc aattaactgc cagctggagt cgtggccata aattaaataa
    57661 ttgctagtgt cgggcaggca gatcaattga ttaatagttt ggctaaagcg accgagtagt
    57721 actttggttt tatgatctgg atcggctagc catagttcaa attgactggc ataggcaatt
    57781 tttttatcag gcagtggaca agctagatgg attgagcgag tgtctagcca attgtcaatt
    57841 agactgctat tgggtattaa atcgttaatc gtgtcggtag ttaaattttt gttattgaga
    57901 ttaagctggt tattacctaa taaactagca gtatccacgg tgctgctagc cggcaaatca
    57961 gcttgccatt gcggttcagc ctgcttaaat agccggacat ctttagcaaa agttgtttca
    58021 gccggtttaa tggttagaat ttttcgccag ctatggtagc cgtctaaact gagattaact
    58081 gtgtaattac ttggtcgtag attttttagc ttaaaggggg tgtttagatt tttaggttta
    58141 ctgaaccaag ctcgtaattc ttcaatattg acctgagcgc cgctgggaat agtctttatt
    58201 tctagtaggc cggttttttc tagcttgaga ttgcgccaat taatgtgata gccggtagca
    58261 taggctaaca ctaacggtgt tattattaaa aacaagagaa taaataaacg cgatagccat
    58321 tgtcgctgaa gaaggctcat aatatttatt caactcgttc aatgtcggcg ccaatcgccc
    58381 gcaatctttc ggcaatttgt tgatagccgc gttctatttg atagatattg tcaatttctg
    58441 ttttaccttc agcaattaag gctgccataa ccatagccat gccggctcgc aaatcgggcg
    58501 aagttagttt gcgaccatgt agcggcgttg gcccattaat aaccactcga tgcggatcgc
    58561 acataattat attggccccc atttgggtca gcatatcggt aaacaataag cggcggtcat
    58621 aaatagtttc atgaacaata ctggatcctt gcgcctgggt cattaaaacg gtgtatggtg
    58681 attgcaaatc agtcgggaaa ccgggatatt cgtgagtttt aatactatag gccgtaatat
    58741 ttttactcgg cagcatcttt agccaattgt cgcctcgttc aaaattaaca ccctgcttac
    58801 gaaaaatatt taataaaaca ctgatatgat ctggaaaaca atcggtaatt aataattcgc
    58861 aacggctggc tgccgccagt aaagcaaagg tgccggtttc aatccgatca ggcatgacgg
    58921 taaaagcgcc accaccgatt gattgtacgc cgttaacaat aatagttgat gtgccggctc
    58981 cgctgatgtc agcgccctgg ctattaagat aatcagccaa agcgacaatt tctggctcca
    59041 tggcgcagtt ctctaagacg gtttgaccgg cggccagaac agccgtcatc attagagctt
    59101 cggttccggt tacactgata gtggtaaaaa agtagttggc tccggataat tgcttagccg
    59161 ccaaatgata gtagccatca gcaaattcta tctgcgcacc aaaagagcga aagccttcta
    59221 aaaataaatc aatcggccga gtgccggctc cgatgacaca gccgccaggg tgtggaaagc
    59281 taacctggcc cattcgggct agaatcggac cgacaaacat aattgaagcc cgtagcttat
    59341 tagctagttc ggcgggaata atcgttgttg ccaggttttt ggtttggatc cggcattgat
    59401 cagtctgtcg atcaactaga ccgcccagcg atttaataat ttctagcgat cgtttgatat
    59461 cttcaatatc aggcagatta tcaataatga tcggttgatc agacagcaga ctggctggaa
    59521 taattttgag cgctaaattt tttgctccgc caaccttgat ctggccacct agcggttgtc
    59581 ggccgttaat aatgaattta ctcataaatt gtaatgattt aattattatc atcatagact
    59641 actttgttga ttctttcaac ttatgttaat ataataaaag attaataata atttattttt
    59701 tatgtttcag cgtcgctcaa cgaaatgttg gggtcgtttt ttgggttggt tatttttagt
    59761 ggcgataatc gccagcggtg gttttttatt tggcttattt tatggtcaaa gggctgaatt
    59821 agcccgtcgg ctttctgatt cgcaggcggt ttttttgggg cgcttaactg gaatttatca
    59881 acaagatcaa caaggtcgtt taattagtga tattgatttt tctttatttt ggcaaacttg
    59941 ggatttgatt aaagctaact atgttgatca ggacaagctg actgataagc agatgtttta
    60001 tggtgctctt agcggtttgg ttagttcggt tgatgatcct tataccgtct ttatgaatcc
    60061 agaagaatcc aagctgttta atgaggattt gaccggcact ttttcgggaa ttggcgctga
    60121 agttggtctg cgtaatgatg ttataactat cattgcgccg ttagctggca ctccagcgga
    60181 acgggccggt ttgcgttctg gcgacaaagt ttacgctatt gataaacaat ctaccgttgg
    60241 cttgactgtt gacgaagcgg ttaaaaaaat tcgtggcgag aaaggcacaa ctgttatcct
    60301 gactattgcg cgcaatggtg ataaactgcg cgatatcagt attactcgcg atactatcgt
    60361 cgtgaagagc gtggccactg actatgatca aactagtggt ctgtatacta ttactgttag
    60421 taattttaat gctgacaccg agcctctttt tcaggcggct gttcaagata ttgaacatca
    60481 gaaaccgcgc ggcttgattc ttgatttgcg caataaccca ggtggttatc ttgatgcggc
    60541 cgtggctatt gccagttttt ggattagcga tggagtaatt gttagcgaac aattttctaa
    60601 tcagtcggca attcaatcac aacatgcccg cggcttagcg ccgttagctg atctaccaac
    60661 cgtagtgttg attaaccaag gcagcgcttc ggcttcggaa attgtggctg gagctttaat
    60721 ggatcatcag caggcgacca ttatcggcaa aacaacttat ggcaagggct cggtgcaagt
    60781 tgttcgcgat ctgcctgacg gttcaatgat taaaataact actgctaaat ggttgacacc
    60841 gaatggtact tctatcaacg ataaaggtat taagcccaat attgaagttg agttgaccga
    60901 agatgatttt aataagaata ttgatccgca gatggttaaa gctcgccaat ttttattaca
    60961 agaggataaa taattattag tttgttcaat aaaaaaacac cgcctcaagc ggtgtttttt
    61021 gtcattggtc taattaacca acctcaacaa tcttagtatt tttgagatgg ctatcaaagc
    61081 ggcgtaattt tttagtacgg aatttgatgg gtccagcggc agtggcaaat aatgtatgat
    61141 cattgccaac agctacatta tttccagcgc gatatttggt gccgcgttga cgaacgataa
    61201 tagagccggc cttagcaatc tgaccgtctt gtaatttaac gccgaggcgc ttactttgtg
    61261 agtcgcgtcc aagcgatgtg gagccaccgg ctttcttgtg tgccataata gtaaaactaa
    61321 acaataaaaa atcagctgtc ttttagctga ccactactat aacaaaggct gaccagcttg
    61381 tcaattgcgc ctggtctgat ataataaaat tattaattat cattagctta aacttattcc
    61441 catgaccaaa ccgttaatca ccagcaaatt ttttaatcgc tggttaatta gtctgttaat
    61501 aatttttggt tttttattgc tgttaaggaa tagttttagt tatcttgatc ctgattttgg
    61561 ctggcattat cgggtcggtc aagagatttg gcaaagtcgt caattgccac ggattgaaca
    61621 tcatattttt ccagtggctg gtcagaactg ggttgatcac gaatggttgt ctaacttatt
    61681 gttgttttta ataaccgatc taggtggtta tttcctgctc agtctgcttt tcgccggctt
    61741 aattattcta acctggtggc tgttagctcg atcaactatc cgacattggc ttgatggtca
    61801 cggtgactgc ctaatattct ggctgctttt aattattggt tttgtagcct gtttgtctaa
    61861 ttttggcatc cgtattcagg aaataactgt tttgtttttg ttgattttaa atttgtggct
    61921 agctaaggtt acccgacaac gattatggcg gccgtgggaa ataattgctt taccagtctt
    61981 tttttggttg tgggcctgtc tgcatggtgg atttttaatt ggtttagccg tgattataac
    62041 ttggacattt tttcgcggaa ttctttatta tttacatcgt catcagcggt cagcggttag
    62101 tccgaccaat ttgctaccac tgaaaactat taaacagtca accatcgttg ctggtttatc
    62161 tttagtagct acctttttaa cgccgtatgg ttggcgtctg tatagttttc tggctgatta
    62221 tcagagtaac cgggcttatt taaagtttat ccaagaatgg cagccaatga ctgaagctat
    62281 ttggagctgg cgaatgcttt atagtttatt ttttggaatg gctttagtgc tattgtattt
    62341 tcatcggcgc aaccaagcca aaactgatcg gcaaattatt caatggttat ggcagcaaga
    62401 ttggtggtcg ttactattgt ctttgataat gtttatagcg gcttggcgtt ctttgcgcca
    62461 tttcccatta ttttttgtag tttctttacc gtggttagcc ggcttttatt atcactcttg
    62521 gcaggattgc cattttcagc cgagtcgttg gccggcttat ctaaaatggt ttatttcggc
    62581 ggtagctatc ttgtcaatcg tcattatacc gataagcact cattggacaa atcagccaac
    62641 cactgattat tgtgccaaat atccctgttc cgctttagat tacttgcgcc aatcgcctta
    62701 tcataattgg ccaaccattg ttgattatgg ttggggtggt tatttttatt ggcaatggcc
    62761 agagaaatcc attttcattg atggccgttt gccacaatac ccttggcgcg accggacgct
    62821 cattgaacat tatagccgtt tctttattga gtcggatatt aaggatgcct taaatgatta
    62881 tgatttatca gttatcgttt tagctcaacc ggcgcctgtt aagtttaact ggttagaaac
    62941 ttggttgctt ggccgtggcc agcaaactac agattatgaa acttctcagg aagtattccg
    63001 tcgagctatg ctgaaattac caaattggcg cttagtttat tctgatatgg tagccacggt
    63061 ttatgtccgg caataagcgg ataactcttg atttatgtct gccggtttat aatgaggcag
    63121 ctatcttagc gagtaatgtc cagagaattg tagactttat tagtagcaat caattagccg
    63181 ttgactgtcg tcttattatt ttagtaaatg gttcaactga tcagtcactt cagattgctc
    63241 aagaattaag tcagcgctgg ccaaaccttg ttagttataa aaattatgaa gtggctagtc
    63301 gcgggtcagc tttattggct tacgctgata ttagtccggc tgattggtgg ggttttatgg
    63361 atattgattt ggcagttgat ttggctagtc tacagcaatt ttttaagttg atggttgatc
    63421 agtcagccga tgttttgtta gcttctagat taatgcccca aagttcttat aagcacaaac
    63481 tgaccagaaa aattatatca gtagtttata atcagttagc tcgttttgct ttaaaaactt
    63541 cagttaaaga tcatcaatgt ggttttaaat tcattagcca atcatggtgg aaaactctcc
    63601 gaccatattt acaagattct cgttggtttt tagatacaga aattattgcc tggacgacca
    63661 aattaggtgg tagaatagtt gaaataccgg ttgattgggt taacaatcgc tatcatattc
    63721 ggccgactaa aattaaatta attcgcgata taagcaattt tttaattaat ctatattgtt
    63781 taagaaagcg cctgcgcgct catcgctcat cataatcgta acttgaatta gaattaattt
    63841 aagaagtttt aagctattgc ctttttaata tttctttgat aaagttgagc cagcaatttt
    63901 gaaattgctt ttttgcgccg ttagctcagt tggtagagca actgcctttt aagcagatgg
    63961 tcactggttc gagtccagta cggcgtactc aatgaatcac ctggcgttaa gccaccctag
    64021 ctcagttggc agagcaacgg ttttgtaaac cgtaggtcgt cggttcaagc ccgacgggtg
    64081 gctccaaatc ataaaaaaac aacccaatcc gggttgtttt tttattgaag aaatcaagat
    64141 tattctttac cttcccattt tttagcagtt tttaggcaac tggtacaaac cttaatagtg
    64201 ccgccgtcaa ctttacaatt ttgtaggttg atgccttggc ggcggatagt tttaatattg
    64261 gagtgggagc ggctagcgcc cttggtggcg ctgcgctggc aaatatcgca ttgcttagcc
    64321 ataattttag atttattatc aaaaaataaa ctaatttaac cattagtctt taactaccat
    64381 agcaagtttt gctaaaataa tcaaggcgtg ttaatctgag aacagttaat aatcaagttt
    64441 atgattagtt tatttcaaat cattattttt ttattgtcag ctattttgca tgaatatggc
    64501 catgcctgga tggcgatgcg cctaggtgat gatactgccg agcgagctgg ccgattaacc
    64561 cttaatccat tggcccatct agatttgttt ggttctttat tattgccggg tatattaatt
    64621 ttaacaggat cgccggtagt ttttggttat gccaagccag tgccgattaa tttacataat
    64681 ttgcgtggcg gattggctgg ccaggctaaa gtcgctttag caggaccggc ggctaatttg
    64741 ttgttagctc tagcggccgc cgggtctatt cgttacttgg cgggcggtct aatctcggag
    64801 tgggggcagc ttttagcggt ggttgtcttg attaatttag tgttgatggt ttttaattta
    64861 ttgccgattc cgccgctgga tggttctaaa attttagtaa ctattttgcc aaaatcgtgg
    64921 cgctattctt atctagcgac cgagcgctgg ggtttcttta ttttattagc gattatgtta
    64981 acttggccgg agctgcttta ttatccggtt agttggctgc tcagcattct cttgtaaagc
    65041 cttgacttcc cgattccttt ctgctaaatt aataagcaac taagcacaat tcctggctcg
    65101 gattgccaga tttattttaa tttttaagaa gaaaacttag gtttttattg ttattatgcc
    65161 aactattaat cagttagtcc gtaagccgcg cacgaccatc agccagaaga gcaagtcgcc
    65221 agccatgcag gttacaatgg attcgttgca tcgcaagcga aatattattc aacaaggttc
    65281 gccatttaag cgcggcgttt gtttaaaagt aaccaccacc acgccgaaga aaccgaactc
    65341 cgctttacgt aagattgctc gtgtccgctt gtctaatggc atggaggtta ccgcttacat
    65401 tccgggcgtt ggtcataatt tacaagaaca ctccattgtt ttaatcaagg gcggccgcac
    65461 taaagatttg cccggtgtcc gctataaggt tattcgtggt gtttatgata ctcagggtgt
    65521 tgacggccgt cgccagagcc gtagtctcta tggcgccaaa gcccctaaag ctaaataatt
    65581 ttgtagacta atatgcgagg aaaacaggcg cctaagcgcg aaatagtcgg tgatccgaag
    65641 tataatgatt tggaaatcgc caaactaatt aattatttaa tgaaaaacgg caagaaaact
    65701 actgctgaac gtatcgttta cgattgtttt gatatcttaa aagaagaaac caagcaagag
    65761 ccgcgtcata tctttaataa aggcttaaaa atgatttgtc cgttagtgga agttcgtagc
    65821 cgtcgggttg gtgggtctaa ttatcaggta ccgcatcagg tgcgcggcga tcgtcgtttt
    65881 actttaggct gccgttggtt attgacagct gctcgggctc gtaaaggtcg ttccatggct
    65941 gaaaaattag ctgccgagat tctggacagc atcaagggtg agagcgcttc ggttaaaaag
    66001 aagctagacg ttcatcgtat ggctgaagcc aataaggctt ttgctcattt tgcccgccac
    66061 tagtttttat taaaaccgac cgatttttga cgagttcgtc ggttttttaa tctgcaaatt
    66121 ttattttact ttaattttta tgcgtgaatt caagttagaa aacactagga atatcggaat
    66181 catggctcac attgacgccg gtaaaactac ttttactgag cgggttcttt tttatactgg
    66241 caagaagcac aaaatcggtg aaactcatga tggtcaagcc gatatggatt ggatggaaca
    66301 ggagaaagag cggggaatta ctattacctc agccgccacg acttgttttt ggaaaaataa
    66361 tcggattaat attattgata ctcccggtca cgttgatttt acagttgaag ttgaacgatc
    66421 gttacgggtg ctagacggcg cggtggcggt ttttgatggc gcggccggtg tcgagccaca
    66481 atcagaaacg gtttggcgtc aggcggataa atatcacgtg ccacgtttat gcttcgttaa
    66541 caagatggac aagatggggg ctgattttat tatgagctta aactctatcc gcgatcgcct
    66601 taactccaaa gcggtcgcta tccaattacc gattggcgct gaagataatt tagtcggtat
    66661 tattgattta ttacttgaaa aggcttatga atttcatggc cagaatggcg agcaaattac
    66721 cgagattgaa ataccggcta atcttaaaga taaggttgcc aaatatcgcc atgagatgat
    66781 tgaaaagatt gttgagttgg atgaatcgtt aacggaaaaa tatcttaatg gtgatgaggt
    66841 taatgttgac gaccttaaga aagctttgcg caagggagtt gttaataatg agatttatcc
    66901 ggttctctgc ggcgctgctt taaaaaatat tggtatccag caggttttag acgcagttaa
    66961 tgaatattta ccatcaccgc ttgatgttcc cgagattgag gcccacgagg ttggcgatga
    67021 tagcaagaaa attattatca aaccagacga caatggtcct tttgtagctc tggcttttaa
    67081 aattgccact gatccgtttg tcggtcgctt gtgctttgtt cgtgtttata gcggcgtctt
    67141 gcgttctgga tcttatgtta ctaattccgc caatggccgc aaagaaagaa tcggtcgttt
    67201 ggtccggatg cacgccaatc atcgcgaaga aattaacgaa atttattccg gcgacatcgc
    67261 cgcagttatc ggcttaaagg aaactattac cggcaatact ttatgcgatg aaggtcaaaa
    67321 tatttgtcta gaatcaatta gttttccgga accggtcatt aaaattgccg ttgaaccaaa
    67381 aactaaagtt gatcaagaga agatgggtat cgctttgggg cgtctagctg aagaagatcc
    67441 gaccttccta gttgagactg atggagagac taatcaaact ttgatttctg gtatgggcga
    67501 gctgcatttg gatattattg ttgaccgcat gcgtcgtgaa tttggtgttg aagctaatgt
    67561 tggccagccg caggtttctt atcgtgaaac tatccgtcaa gatgccgaag ccgaaggtaa
    67621 atatatcaag cagtcaggcg gtcgcggtca gtatggccat tgctggttac gcatcgggcc
    67681 acaggctgaa ggccagggct ctgagttcgt tgatgaagtt aaaggcggcg ttattccgcg
    67741 cgaatatatt ccagctattg aaaaaggctt tcgtgaagct ttagataaag gtgttttggc
    67801 tggttatccc atagttgatg ttcgatgtgc tgtttatgac ggttcctacc atgaagttga
    67861 ttctagcgag gcagccttta agatggcggc cgttatggcc tttaaagatg cctgccgtaa
    67921 agctcgtccg gttttgttag agccaattat gaaagttgag gtaactactc cggaagaata
    67981 catgggcaat gtcattggtg atttaaatgc taaacgcggg cagattgacc atatgtctga
    68041 tcgtggtaaa ctaaaaatta ttgatgctaa agtaccgttg gcggaaatgt ttggttatgc
    68101 cactcaattg cggtcaatga gccaaggccg cgctaattat agcatggaat ttttacatta
    68161 taacgaggtg ccaagaaatg tcgccgagca gattatcggc aagtccggta aataagtagc
    68221 tggccgtggt ttggctctcc ccctagccta tgattaagaa tattgatcaa attttgcgtt
    68281 tagctcaatt aaccggcgac cggttgattg ttgctgacca acagaatccg gagcgtagtt
    68341 tggccgtgct atcgttgagc gattatgaag aattattgtc tcttgccggc ttgacaaaga
    68401 agcagggctt tgctaaagta gacaataatg ttgccagcca agaggctggt aatgttgctg
    68461 ttaattccag ttttgtgcaa aaacttggcc aaagccaggg taaaaaatca ccgtggtcaa
    68521 ttccggctag ccgacaggcg gcacaaaaaa atattgatga ttaataattt tatttaataa
    68581 aacttaataa ccacccagct ggctaagcag ctgtctggtg ttaatcaaga aactatgact
    68641 gacaagtacg aacgcagtaa gccccatgtc aatgtcggca ccatcggcca tgttgatcat
    68701 ggtaagacca ctttgactgc ggctatgttg actgttttaa ctgccgctgg ccgcaagacc
    68761 agccaaaaga gcgttgatca gattgactcc gctccagagg aaaaggctcg cggtattacg
    68821 attgccacta ctcatgttga atatgagtca gaaaatcgtc actatgctca cgttgactgt
    68881 cctggtcacg ccgattatgt caagaatatg attactggcg ctgctcagat ggacggcgct
    68941 attctcgttg tggctgccac tgatggtcca atgcctcaga ctcgcgagca tatcttgcta
    69001 gccagacagg ttggcgtgcc ttatattgtc gtctttttaa acaagtgcga catggttgag
    69061 gataaagagt tgattgattt ggttgaagaa gaggttcgtg atttattaaa caaatacagt
    69121 ttccctggcg ataccacccc aattattcgt ggttctgctt tgcaggcttt acagaatcca
    69181 actggtgagt atgctaaatc tatcatggaa ctgattgaca ctttggatac ttatattcct
    69241 gaaccacaac gccaagttga caaagatttc ttaatgccga ttgaagatat tttttccatt
    69301 gaaggtcgcg gcactgtggt taccggtaga attgaacgcg gtatcgtcaa ggttagcgac
    69361 gaagttgagt tagttggctt aggtgatact cgcaagattg tagttaccgg tgtggaaatg
    69421 tttaacaagt ccttggatca aggtatggct ggtgataatg ccggtattct tttgcgcggt
    69481 atcaagaaag atgaagttga acgcggtcaa gttttagcca agaccggctc catcactccg
    69541 cataccgaat ttgaggccga gatttatgtc ttaaccaagg aagagggtgg ccgtcataag
    69601 ccgttcttta agggctataa gccgcaattc tatattcgca ccactgatgt gaccggtgaa
    69661 atagaattac ctgaaggaac tgagatggtt atgcctggcg atactgttac tttaaaagtt
    69721 aaattaatta ccccaatcgc gctagaggac aagatgcgct tcgccattcg cgaaggcggt
    69781 cggactgttg gttgtggtgt gatttccaag attattaaat agaagataga tagtcctgct
    69841 gccacgcctg gtggcaggat tattctgttt ttttatagat taataaacag ctaaaaacat
    69901 gcctgacaag aaagccgcac ctgccgacta caagcagagg attagaatta aaattcgcgc
    69961 ttttgatcac aagattatcg atcaatcaac taaaacgatt atcgatactg tgcaacggac
    70021 tggcgccaaa atttatgggc cgattccgtt gccaactgaa aaaaagaaat atactgtcaa
    70081 cagctcaacc tttgttcata agaatgctcg cgaccagtat gagatgcgca ttcataagcg
    70141 tttattagat attattgatc cggctactaa gacggtggaa tcattgatga atttgagctt
    70201 accagccggt gttgatttgg aaattaagat gtaaaatacc aatttatttg gtttttagat
    70261 agtttttggt ttagtcaggg tttaaaaaac ctcgcggatt ttttccgccg agaagtcttt
    70321 gaaccgacag ctgaaccaat gggagaaatt tttcgttaaa ttcagttcaa caaaattaga
    70381 tcaacctcta tggttaattg gttttgcggt gctggttttt gcggaaaagc cagtttttat
    70441 ttttaaggtg atccggttat gaagtttatt ttaggcaaaa aattgtccat gacccagatt
    70501 tggcgcaata accttgttgt cccggtgact cgtattcagg tggaaccctg cgtgattacg
    70561 cagattaaaa ctgttgcaac tgatggttac cgagccgttc agttggctac cggtaagcgc
    70621 aaaatcaaaa atatcgccaa gccgcaacgt ggtcattttg gtgcttgggg caattttcaa
    70681 tacctgcgcg aattcaattt agatcaagag aaggaacagc cagaagccaa ttggcaagct
    70741 ggacaacaag ttgcgattaa cagttttatt cccggtgatc gggttcaagt aacatcaact
    70801 agcaagggcc gtggttttca gggcggcgtt aagcggcatg gttttcgtgg tcaaggtcgc
    70861 acccatggtg ttaaagatca gcagagaatg cctggttcag tcggagcgac tggtccagcc
    70921 cacattttta aaggaacacg catggctggc cgtatgggtg gtgagcaagt gactattaag
    70981 aatttagagg ttgttgaggt tgatgaagct aatagtttta tttatttgaa gggggcggtg
    71041 cccggttgga ctaatggttg ggtgtcaatt gttggatctg gcgatttatt gattgtcgag
    71101 ccggcggctg ccgttgaaga agttgagaca ccagttatgg aagatattgt cgaagcagct
    71161 gctcatgata ccgaaggaag tgttaatcca gaagaaaacg ctgattcgat tgttgaagat
    71221 gctattgaat cagcaactaa ggaggaaccg gcggccgaaa ccgaggctga tgctgaagaa
    71281 aaagcagtgg ctgaggaagc ggcggccaat gaaccagcag cttaatttgc ttagctaaat
    71341 taaccgttat gattaaatat caagtttaca atcaacaggc tgaagtcgtt ggcgatcaag
    71401 agctaagcca agaggttttt ggtgtggcta ttaatccagc tttagttcat caagcgatgg
    71461 tcgctcagct agccaacact cgttcagtga ttgccgatac taaggatcgc agcgaagtca
    71521 gaggtggcgg caagaagcca tggaagcaga aaggcactgg ccgcgctcgg gctggttcta
    71581 accgtagtcc gatttggatt ggtggtggtg tcactttcgg tccgactaat gatcgtaatt
    71641 ttactaaaaa gattaataaa aaaatgctga gtaaggccat ctgtatggtt tttagcgatc
    71701 gggtcgccag tcatgatttg attattgttg atgatttgac ggttaaagac ggcaagacca
    71761 aggaattaaa agcaattatt gaaaaattat tagcggctga tcagcaaggt agtcaagtgg
    71821 ctaagccgcg gactcttatt ttgaccggca gcagcattga ctctgtcaag ttggctggcc
    71881 gcaatttgcc acgagttcag attttgaatg atcagaatat taatgtggtc agcttgctgc
    71941 gggctaataa atttattatt agtcagcagg cgattgccag tctgcagcag cgttacggca
    72001 ctgtggccgc taaataagct atttgttaag atatcagtat ggaaaagcag aataatagta
    72061 cggcggttgt tgatgagaag atcgcccaag ccgatcgtca gacgattcgc cgcggccaat
    72121 tggctggagt attgattaag ccagtggtga ctgagaaagc gacggctgtc aatcatttaa
    72181 atcaatatgt ttttatggtg gctaataagg ccaataagat tgatattgct aaagctgtgc
    72241 tggtttttta tggtattgaa ccggtcgcgg tcaatattat caatgtggct ggcaaaaaag
    72301 tagcgcgcgg caagatcaag ggccagcgta atgattggaa aaaagccatt gtgactttgc
    72361 cggctggcaa gaccattcag atttatgagg gtgtgtaata tttactaagt taagcgatga
    72421 ttatgcctat cagaaaagtt aaaccgacaa cttctggcct gcgtggatcc agttttgata
    72481 attttagtga tattaccagc accactccgg agcgtcggtt attagttatt cgtaaacaaa
    72541 aaggtggtcg caataatcaa ggtaaaatta ctgtccgtca tcgcggcggc ggccacaagc
    72601 gttatattag aatcgttgat ttcaagcgcg ataaatttga tattcctggc caggttatga
    72661 ccattgagta tgatcctaat cgcggtgctc gcttagcttt agtgcgttat gccgatggcg
    72721 aaaaacgtta tattattgct ccagttgatt tgcaagttgg tcagacaatt ttaagctcta
    72781 agaaattaat tgaaattaaa acgggtaatg ctttgccatt agagtatatt cccgccggca
    72841 tgccggttca caatgttgaa ttacggcctg ggttcggcgg caagctagcc cgatcggctg
    72901 gtaacgcttt gtatgttatg ggcgtggaaa agaaatttgc tcaattaaaa atgccgtctg
    72961 gcgaaatccg tttagtcagc aaggcctgtt tgtgcaccgt cggtcaagtt ggcaaccagg
    73021 acattgcccg gattaatttg ggcaaagccg gccgaaatcg tcatttaggt attcgtccga
    73081 cagttcgtgg tactgccatg aatccggttg atcatccaca tggcggcggc gaaggcaatc
    73141 agccgattgg tttgaaatat cctaagaccc cttggggtaa gcatgctttg ggtgttcgca
    73201 ctcgcgaccc gaaagcgtcc agtaattatt taattatcca acgccgcaag aagaagcgtt
    73261 aaactttgtt aatttatatt gactagccta tgtccagaag tttaaaaaaa ggcccttata
    73321 ttaatgaacg gttgattgcg aaaatcaaga aattggccgt tggcgataaa actgttatca
    73381 aaacctggga tcgtgcttgc actatcactc cagaaatgat cggttttgtt atcggtgttc
    73441 ataatggtcg tcagcatata ccggtgttga ttagcgagag catgatcggc cacaagcttg
    73501 gtgagttttc accaactcgt aaattcgtca gtcatggcgg caagatggcc aaggctcagg
    73561 ctaagggcaa gtaatttatt agctaactaa gattcagtat atggagatta ccgcacaagc
    73621 caaacatatt cgcatttcgc cccgcaaagc cggcctagtt gctagtttag ttcgcggccg
    73681 ccaagtcaat gaagcgttag ctcaattaaa atttgtcaat aaaaaggccg gtcgttttat
    73741 tagcaaatta ttggtatcag ctattgctaa cgccaagcat aattatgatt tgaccgccga
    73801 caatctgact attaaacaaa tttgggtcgg caaaggcgct acttttcatc gctggatgcc
    73861 taaggcgcac ggtcgggcca ctccaatcaa taaaaccact agccatatta ctatcgttct
    73921 gggcgaaatt aaggatagcg gtttaaaaat tgctcggaga gttgaggctg aagtgccgat
    73981 taagttaagt gaattagcca gtaaaggcga gagcaagaat ttagataagg ctgatcaggc
    74041 taaagaatta gctaatgaag ccgaagccaa ggctgagacc tttgaacaag cggccaagcg
    74101 tgatggtggt tctaagtctg gttttgtgaa gaaagttttt cagcgtaaag ccggttaatt
    74161 aaattattct tatgggacac aaagttaatc caaaaatttt ccgtattggc atcattaaga
    74221 cttggccatc caaatggttc aagcaaggcc agcagtatat caagaattta cagcaagatg
    74281 tcaagttgcg tgattttctt ttcagtaatt tacgggtcgc cggcttggat cgggttgaga
    74341 ttgaacgtag cgctaataag gtttcagtca gtctgttcgt ggctaagcct ggcttagtta
    74401 tcggccatgg cggtactggc gctgaagatt taaagaaaaa attacatagt caattcttaa
    74461 agaattttcg tttgcatgat atcagtttaa atattggtga agttaatcgg cccaatttga
    74521 gtgccaatat tttagctcag accatggctt tagaattgga acagcgggcg ccttttcgca
    74581 aaataatgaa acagaccatc aaccgcgctg agcgttctgg cgctttaggg gttaaggtta
    74641 tggtcagcgg acggttaaat ggcgctgaaa ttgctcggac cgaggtttta acttctggta
    74701 aagttccttt gcatacttta cgcgctgata tagattatgc ccgcagcact gcctatacca
    74761 cctttggtac tatcggtatc aaagtctggg tttatcgcgg cgaagttttt gataatattg
    74821 aggaggttaa aggcgaagtc gttggccagc ggctgcgtta gtttataaaa gttaagcaga
    74881 ccagtatgtt aatgcccaag aaaactaaat atcgtaaatc acataaagcc aagcgcggcg
    74941 gtgtggctac tcgtggcact actatcagtt ttggcagtta tggcttaaaa gccactaccg
    75001 cttgctgggt gaccgctcgt caaattgagg cggctcgtcg agttattacc cgttatgtcc
    75061 agcggactgg caaattatgg attcgtattt ttccagatcg tccggttact gctaaaggtg
    75121 gcgaaattcc gatgggtaag ggtaaaggag ccgttgatca ttatattgtt ccagtcaagc
    75181 ccggtgttat tatgtttgag attgaaggta ttgatgaaaa gcgtgcccgc gaggctatga
    75241 atttagctgc ttacaaatta ccagttaagt gtcagtttgt cggccgcgat cagcttaact
    75301 aattgtttat tgtgaagact atggaatatc gagatttaca aaccaagagc gagcacgaat
    75361 tgcaactgat gttggccgaa aaacgcgccg aattaaaaga tctgcgtttt aagaatgcca
    75421 gtcatcagct gaaggcagtg cggcatatta gagcggtccg ccaaacagtg gctcgcctgt
    75481 taactttatt aaataatcgc cagaaataat tatgaccaag cagaatcagg tcgctgcccc
    75541 taaacaggcc atgcgcaaaa aatttcgtgg tgttgttgtc agcgataaga tggaaaaaac
    75601 cattgtggtt aaagtggaac gggtgaaagt tcatcccaag tatcacaagc gttatactgt
    75661 tagccgcaaa tataaggttc atgacgaggc tggaacttgc cagcttggcg atcaggtaac
    75721 ctttgtggag tgccgtccgt tatctaaaga taagcgctgg cgagttttgg ttaatagccg
    75781 ttaatttgtt aattagttat catccgtatg attcaagttc aaaccgtatt aaaagtagct
    75841 gataactctg gcgctaagaa agtgcagtgc attcgtttaa tgaagggtta tcgacggcac
    75901 tatgctagtg ttggcgatat gatcatggtg actgttaaag aagcggtgcc gcatgcggct
    75961 gtgaaaaaaa gcgatgtctt gccggcggtt attgtccgga cgaccaaaga aattcgtcgg
    76021 gccgatggta gctatatccg ttttgatgaa aacgcctgtg ttattgttga taaaaaaaat
    76081 catgaaccgc gcggaagccg tatttttggg ccaatccctc gtgaagtccg ccgggccggc
    76141 tttgttaaga ttgcttcatt ggcgccggaa gtcttataat cagtaattaa tccagttatg
    76201 aagattaaaa agaatgataa agtattaatt ttagctggca aagatcgcgg caagaccggc
    76261 aaggtattgc aagcttttcc tcgtcagcaa agagccagcg ttgaaggtat caatttactg
    76321 ataaagcata tccggccgca acgccgcggc gaacatggtc agcggattga atttccagcc
    76381 ccgctgcata tttctaattt gcaattgatt tgtccgcagt gcggtaagcc ggcgcgggtc
    76441 ggttataaat atttggcagt caaagagggc gataaaacca aggctcgcaa ggtcagattg
    76501 tgtcgccgct gccaacaatt gattgactag taaacattat tttatgcgct tgaaagaaaa
    76561 atacaacaaa gaaataatcc cggccttaaa aaaggaattt aagtacgcca atctttttga
    76621 tgtacctaaa ttaactaaag cggtggttaa tgttggtgtt ggccgattta ccaaagacaa
    76681 ggcctatatt gagaatgttt gtgaaacgct agctaagatt accggtcagc agccaatact
    76741 aaacccggct cgcaaatcaa tttccgcttt taaaattcgt gaaggcatga ttgttggagt
    76801 atcagtaact ttgcgcggtc agcgcatgta tgattttatt gaaaaattgg tggctgtttc
    76861 gttgccgaga attcgtgatt ttcgcggttt gaacagcaaa ctagttgatc gtagcggtaa
    76921 tttaagtatt ggcattaaag aaagctcttg ttttccagaa atccgctccg atgaaattga
    76981 aaaatctcat gggttagaaa tatgtttggc aaccactgcc aaaactagag aggaagggtt
    77041 agccttgttt aaattgatgg gttttccttt caagactgaa taagttttat taactactag
    77101 aagaagacta tggccagaag agcgttaatt gtcaaagcca aaaaaacccc taaattttcc
    77161 agtcggattg tgcgccgctg ttggcgctgt gggcgtaatc atggctatat gcgggatttt
    77221 ggaatctgcc gcatttgttt tcgggagtta gctagcaatg gcgacttacc gggtattact
    77281 aagtccagtt ggtaaattat atatttttac aaattcctaa gttcacctta ttatgataga
    77341 tccaatttcc gacatgctgt ctcgtatcag gaatgccgcg gctgttaaaa aggccgaggt
    77401 tgtcctgccg atgagcaagt tgaaatataa tattgccaaa atattggaac gcgaaggctt
    77461 tctggctggc gttgagatta ttaacccaga ttcggctaaa aataaaaccg ccgcttttgc
    77521 tgaattgcgc ttagttttga aatatcacaa caacggtcaa tcagtcatta cggccatcaa
    77581 ccggattagt cgtccaggtc ggcgagttta ttctggcaaa gaaaaattgc cagtagtggt
    77641 taataatttc ggtatcgcca ttatttctac ttccgctggc ctgttgacca ataaagaagc
    77701 gcgccagaaa ggaataggcg gtgaagtttt gtgtagcgtc tattaatttg ttgataattg
    77761 attaagcata tgtcaagatt aggaaaatta ccgattattt tgccagccgg caccactgcc
    77821 gttttggata agggaattct aacggttaat ggtcccaagg gccaactcca gcaagccatc
    77881 cacaaattta ttaaggtggt tattgaggac ggcgctatta tggttaatgt tgacaataaa
    77941 gacgaacgtc agcagcgagc tttttgggga ttatttcata agttgatcgg taatatggtt
    78001 atcggtgtta gtcagggtta tgaaaagaag ctggagatta acggtgttgg ttatcgggtt
    78061 agtattgctg gccagaaact aactctcaac ctaggttttt ctcatccagt tgattttatt
    78121 ttgcctgatg gtatcaaggg gcaggtggag gccaatatta ttactttgac cggtattgat
    78181 aagcaattgg tcggggagat tgccgcccag attcgtcgcc agagaaagcc ggagccatac
    78241 aaagggaaag gtatcaaata tattgatgaa gttatccgtc gcaaggccgg caagacttca
    78301 gctaaataag ctagctaatt ttagaatgat atgagcagta caagtaaaac agctaaaaaa
    78361 attagacgcc ataatcgcgt tcgggctaaa attagcggga cagctgattg tccgcggttg
    78421 agtgttttcc ggtcgaatcg gggtatgtat ctgcaagcca ttgacgatac taagggttta
    78481 acgctagcta gcgctcatag ccgtgaggtt aaggttaagg gcaataaaac tgaagttagt
    78541 caggctctcg gtgttttaat ggccgaaaaa ttattagcca aaaaaattag taaaatagtt
    78601 tttgatcggg gcagtagcgc ctatcatggc cgtgttcaag ctgccgctga aggcgctcgc
    78661 caggctggct tgcaatttta gttaattagc atcaagtatg actgaagtta aagatactat
    78721 cgtcagcgaa aaacaagccg ttccagaggc tgtgtccgct gtcactgaaa atcaggcgac
    78781 tgaacgtcct agccgcagta gtaatcgcgg tcaacgaggc aatgctggcg gccgcggccg
    78841 cggtgatcgc cggtctaatg atcgtcaatc tcaggatgag tttgatcaaa agattttgga
    78901 catcgctcga gttactagag ttatggcggg cggtaaaaga atgcgtttcc gcgcctgtgt
    78961 cgctattggt gatcgcaagg gacgcgtcgc tattggttta gccaaggggc tagatgttac
    79021 tattgcagtc aacaaagcag ttaatcaggc taaaaagaat ttggttactg tgccgatgat
    79081 tgataaaact atccctcatg aaattaaaca gaaatctgat tcggctgtta ttatcttaaa
    79141 accggccaaa cagggtcgcg gtattattgc cggtagcgtg gtgcggatta ttttagaatt
    79201 agccggtatt aataatgtta ctagcaagat gctaggatcg cataataaga ttaataatgc
    79261 gaagtgtgtt attaaggcct tgcgtaacct gaaaaaaccg gcggccaatc atcgttcagt
    79321 tagtaagcca gccgttgcca ttaagaccga aaccggggcc gaatagtaaa ttaattaatt
    79381 tctttttatg ttgacactcc acacattaaa accggcgatt ggttccagca aacagcgcaa
    79441 gcgagtcgga cgaggtaatg cttccggaca cggtaattac agcggtaaag gcatgaaggg
    79501 acagaaatct cgttcgggcg tcagcggtat taagcgcatg gccctaaaaa aggcgatgtt
    79561 acaaattcct aaattgcgtg gtttccaatc caataagcct aaatatcaag tagttaattt
    79621 atccaccctg agccgcagtt ttgccgatgg tgctataatt tcggccgata gcttacttaa
    79681 ggccggattg gttagtcagg ccaaaaatat taagattttg gctaacggtg aattaaaatt
    79741 aaagaattta cagattaagg gtttaaagct cagcgcggcc gctcaggaac agctgactaa
    79801 aatgggtgga aaaatagttg aataaattac ttttatggaa aaattactaa aaatttggcg
    79861 gattcgtgaa ttgagaaaca atattttgtt tgtcattgcc atgctggtaa tttttcggct
    79921 ggcggctcat ttaccagtgc ctggcgtcaa tgctcaagct ttgcgtgatc tgtttgcttc
    79981 caatcaattt ttaggtttga tgaatctgtt ttccggcggc ggtatgcaaa atttttccat
    80041 cgttatgatg ggagtagcgc cgtatattac ggcttcgatc atctttcagc tgttaggaat
    80101 gattgtcccg tcaattgaag aaatgaataa agaagaagct ggtcggcaaa agattaatat
    80161 gtggactcgt tgggccacag tgccattggc ggtgatccag tcttacggca tgattgcttt
    80221 attgcgtcgc tccagttatc agattctcgg ctcaattgat tggcttaact atgttaccat
    80281 tatcgttacc attacagctg gcactgtttt tttgatgtgg ctcggtgaat tgattaccga
    80341 aagaaagacc ggcaacggca tttctatgtt gatttttgcc ggtattatcg ctggcgtgcc
    80401 gcagtcaatt cagcaattaa tcttgactta tgatagttcc aagctgttta tggttatcgg
    80461 ttttgttgtt attgcgattt taacgattgt aggcgttatt tatattagtg agggcc
//
LOCUS       DAOPAI010000002        69867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_439_length_69867_cov_5.943018, whole genome shotgun sequence.
ACCESSION   DAOPAI010000002 DAOPAI010000000
VERSION     DAOPAI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 69867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69867
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_439_length_69867_cov_5.943018"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKV49_00455"
     CDS             <1..682
                     /locus_tag="PKV49_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22129.1"
                     /translation="AITLVGGVNGFIPQAEAGTQCFIRFNVLDSKIYSLETVETILLK
                     ANLINRGDLTGSTQQERRMPFDSKVGDSQVVNETLDNLQINLCGKVDGCDTRTLDMEV
                     ICELSTSPTGNTIRDAQLAYRSRITEMNACQQGFFKKIRALNSQYVRDVRAANVQYYQ
                     QLAPRTAALLKSYRAADDKDKRAILDEYITHNQILRRDLYTTQTIIFETFINSRTALQ
                     NNFNFCGS"
     gene            679..1545
                     /locus_tag="PKV49_00460"
     CDS             679..1545
                     /locus_tag="PKV49_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPL22130.1"
                     /translation="MIDFKHILSVMNLDKPLVVFDLETTGLALAYDRIIELAFVKILP
                     NGRVIEKDIFFNPEIDIPSEVTAIHGLTNADLVDKPLFREQAQEIWDVFNDCYYGGFN
                     IANFDLPMLRREFLRAGYDFNFTKKQIIDSKIIYHAMEPRDLEAAYKYYCQKKLVDAH
                     SALADVHASVEILDQQLIKYDYQTIRQISEAPDDRYVDEEKRFYWRGGEAYLNFSKHK
                     DRSLADVVKVDPGFLQWIIGADFSPETKQIVERALQGEFPKKSGAVAVTKDNLPLTKK
                     SASGFTDKPKLF"
     gene            1550..2578
                     /gene="ruvB"
                     /locus_tag="PKV49_00465"
     CDS             1550..2578
                     /gene="ruvB"
                     /locus_tag="PKV49_00465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPL22131.1"
                     /translation="MSVNSNSRDHLLAADLSDASDQDLDLSLRPKKLSEYVGQPAVRQ
                     SLEIFLQAAQSRGENIEHVLLYGAPGLGKTTLAYVIANELGANLRVTAGPAIEKSGDL
                     AAILTNLQDGDLLFIDEIHRLPHSVEEILYPAMEDRVLDIVLGKGPSARTLRLDLPHF
                     SLIGATTKVSSLSAPLRDRFGIVYRLDFYNSNDLQKIISRNAKILQLEIEPQAQSLIA
                     DRARRTPRIANRLLKRVRDYCQVRGTGAITLALCRQAFDLLAVDDLGLDEVDRYLLTT
                     IIEKFGGGPVGLNTLAAATGEELMTIEDVYEPYLLQLGFLERTPRGRKATVLAYNHLG
                     IMPPAGLF"
     gene            2581..3642
                     /gene="queA"
                     /locus_tag="PKV49_00470"
     CDS             2581..3642
                     /gene="queA"
                     /locus_tag="PKV49_00470"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPL22132.1"
                     /translation="MDLANFDYYLPPELIAQTPAEPRDQARLMVVDRQTGFRQGAIFS
                     QLPDFLQIGDVLLINNSKVLPARLLGHKVGSGGRIEILLEQKLSQPNNGEYWRCLVGG
                     TRPGIGLVVDLGDNLQAEIMAAETDGVFVVKFNQTGTDFWSAIYRLGQMPLPPYIKTD
                     QIGLQRAVNDYQTIYAKADKIGSVAAPTAGLHFTETLLRQLKNQGVDIIEATLHVGLG
                     TFAPVRQQNIKDQRLHDEMVDIRAETINKLINYHRQGRRIIAVGTTAVRILEGVVAKF
                     IDKPADKDWLAPINLFIYPGFKFKLLGGLVTNFHLPKSSLIMLVSAWAGTKLIKQAYQ
                     EAVEQHYRFFSYGDAMLLV"
     gene            3721..3978
                     /gene="rpmF"
                     /locus_tag="PKV49_00475"
     CDS             3721..3978
                     /gene="rpmF"
                     /locus_tag="PKV49_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPL22133.1"
                     /translation="MVQMKKRTKSETRGRRSHHALKAKTLVKCPQCGQMALPHTACAF
                     CGTYKGRQVIKVKSAAGKTAKPAKAAKPATKDKATKAKKAE"
     gene            3983..4456
                     /gene="nusB"
                     /locus_tag="PKV49_00480"
     CDS             3983..4456
                     /gene="nusB"
                     /locus_tag="PKV49_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPL22134.1"
                     /translation="MANRHLARTIAMQTLFCWDFNGQKDKLEDLVKESFQQFAPEFDD
                     HGFVNDLVKGVVANLPAIDNYIVKYATEWPLDQITPVDRNILRIGTYELVFDQNIPAK
                     VAINEAIEVAKSFGGDASGRFVNGVLGAVFKEIPPKDIDKQPKGQPETAEAMPAE"
     gene            4462..5157
                     /gene="rnc"
                     /locus_tag="PKV49_00485"
     CDS             4462..5157
                     /gene="rnc"
                     /locus_tag="PKV49_00485"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPL22135.1"
                     /translation="MTNQQQLARALGVEFKDDKLLTQALVHRSYLNEHNNFPVSHNER
                     LEFLGDAVLELIVTEFLFLNYPDKAEGELTDWRAALVNAKTLSQVAKEIHLDDYLFLS
                     RGEARETASKGRQYILADAMEALIGAIYLDQGWNVAQRVINQLVLTKLGNIIKNKLYL
                     DPKSYFQERAQETYGFTPHYEVLSSSGPDHEKTFEVGLYLDDRLISRGHGMSKQEAQV
                     DAAENGLKQEGWR"
     gene            complement(5096..5623)
                     /locus_tag="PKV49_00490"
     CDS             complement(5096..5623)
                     /locus_tag="PKV49_00490"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPL22136.1"
                     /translation="MQKPAAKKLAIRRHPVRAALFRAVLLLIIDRALKIISLIYWSQN
                     HVQLSGSFSLTYNLNRRLSFSLPGNGPLFNWLLIFLVIGLTIYSRQAIKKQPSRLDGW
                     LWLLAGAYSNLYDRLVYGGVIDYLTSWWTVFNLADVLIGIGLISLLWPKTNQNSSASP
                     PASNHSQQRPPEPLA"
     gene            complement(5596..5964)
                     /locus_tag="PKV49_00495"
     CDS             complement(5596..5964)
                     /locus_tag="PKV49_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HPL22137.1"
                     /translation="MKPETIQEIKAELLRIKQETEEALKVFATKDSGNANDFTSTFPD
                     YGEENDENAQEVGQYSTNIATENILEKTLRDVKSALKKIDQNTYGICKYCHKEIDEKR
                     LLARPFSSSCVKCKSQLQKN"
     gene            complement(6044..6508)
                     /gene="tsaE"
                     /locus_tag="PKV49_00500"
     CDS             complement(6044..6508)
                     /gene="tsaE"
                     /locus_tag="PKV49_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPL22138.1"
                     /translation="MLLKPKNNQLLVEYYAPTVRQTASLARQLAKNWQGGQIIALSGE
                     LGTGKTSFVQSVAKYLKIAQPLVSPTFTIWQEYDCQHPTIKKLLHIDAYRLTGGQDLQ
                     NLGITDWVKQPNCLIIIEWPEIVADWLPQQAKIINFQHQPPGRLISWLEPRI"
     gene            complement(6486..8180)
                     /locus_tag="PKV49_00505"
     CDS             complement(6486..8180)
                     /locus_tag="PKV49_00505"
                     /inference="COORDINATES: protein motif:HMM:NF023671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosyltransferase"
                     /protein_id="HPL22139.1"
                     /translation="MTFLDIQIYSGIKLILVLFAILATYLIIKKKNPLYLLIAFGLTS
                     ASTYFLWSNNLQLPLWGLQGDELTIAAMFNTFAKIGLGADFSYHHLPPFYPPASFWLF
                     GLIGRFFHWNGIIMIKAAATTFLLIFPAVVYLLQRRLFSNILDDNRLNTLLAVLSPLL
                     IMSVLDKDLMLGKPYEIVSAVMVILWTISLHYQAIGQRWKIWLPIVYGLAAGLIFMTY
                     YLWLIFAAIAFILMGLLENNQHKLRYFWRLGQTAVVGLIATLPFTGPLIGSYLKNGLE
                     NWQTVFFTPSGLDLWLPMFQLTSWTNLILLFGLATLIYYRRQPIAKQLLYLLSTSFIW
                     WVGGLLLLLIWHKPFQEFRGFYVWAPTILALGAAYGLSRLWNYYQVNQKANYLITLAL
                     LSLLYLISQSFFGFFIDHPAIKNRRLESRQADPAIIQLANYLTANPISPHELTLETTP
                     QLLAFTPINNLLYFNQHNGHPASIFSERYNYVHSLAAAKTAAELEQKIKQCPFGKLKR
                     LIFYSDQKYYYLYFHLDEFISGIKEAELKFDRQLFISPYFTTTYQNNGYYVIETQE"
     gene            complement(8192..8266)
                     /locus_tag="PKV49_00510"
     tRNA            complement(8192..8266)
                     /locus_tag="PKV49_00510"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8229..8231),aa:Val,seq:tac)
     gene            complement(8351..8725)
                     /locus_tag="PKV49_00515"
     CDS             complement(8351..8725)
                     /locus_tag="PKV49_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22140.1"
                     /translation="MLEQFRPQDSQRESQEATTENKVGLSLSLCIKNILEGKVKEEEV
                     KEIIAGTSFAPEKFDELIDLYKKSYWYDNPEEGEAIARRLFAAGKIKQPRMEGGKIHS
                     IAEGHWLDAEDAEEEYKKRIME"
     gene            complement(8785..8871)
                     /locus_tag="PKV49_00520"
     tRNA            complement(8785..8871)
                     /locus_tag="PKV49_00520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8835..8837),aa:Leu,seq:gag)
     gene            complement(8879..9277)
                     /locus_tag="PKV49_00525"
     CDS             complement(8879..9277)
                     /locus_tag="PKV49_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPL22141.1"
                     /translation="MTETKLVVAATEEQPTKTTLGDLKPGMTVRIYQRIKELNPKGEE
                     KERIQYFDGLVIACKHGREAGGTVTVRKVSNGVAVEKIFPINLPTINKVEIKKQAIVR
                     RAKLTFLKKGYKKRLKDISEKKTAIAKKAA"
     gene            complement(9282..12239)
                     /gene="uvrA"
                     /locus_tag="PKV49_00530"
     CDS             complement(9282..12239)
                     /gene="uvrA"
                     /locus_tag="PKV49_00530"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPL22142.1"
                     /translation="MTTKTVSQDWLKIRGARVNNLQNIDLDIPRGQLVVITGLSGSGK
                     SSLAFDTIYAEGQRRYVESLSAYARQFLGLMDKPDVDLIEGLSPAISIDQKSASNNPR
                     STVGTITEIYDYLRLLYARIGQPHCPVCGDPIKANTVDEIVDQIIKQATGQKIAILSP
                     LIRDKKGEHKNVLLAIARAGFSRVRWDNNLYDLEEAQNLTVDKQKKHNLSIVIDRLTI
                     NNDKSSYSRLTEAVDKALDLANGLVATLDLNQTEAEEKIYSQHFACPRCNINLPEIEP
                     RNFSFNSPHGACPHCTGLGTKLEIIPELVFNNNLTIAQGAVRPWSHYGYSRQLWMTRV
                     IGAVANEYGFDLNTPLNKLTAKQLDILMNGTGDLVYTLPSTAGMNQLNVKFEGVIPNL
                     YQRYQQTESDFVRTEIEKYMRTIPCSVCHGQRLKPEFLAVKLAGQSIYELTEQTIAQN
                     YTFFKQLIANKKLTAKQTQIGRLIFKEIISRLQFLLNVGLDYLSLNRPATTLSGGEAQ
                     RIRLATQIGSALTGVLYVLDEPSIGLHPKDNDKLTDTLKNLRDLGNSVLVVEHDKATM
                     LAADHIIDVGPGAGKNGGQIIAQGTAKEIMANPKSLTGQYLSGKKSIPIPKKYRAGNG
                     HYLTIIGARQNNLQNLTVNFPLGKFIAITGVSGSGKSTLVTDILARALKQKFYQALED
                     PGQHERIDGLQYINKIIETDQSPIGRTPRSNPATYTGAFTPIRDLFASLPEAKLRGYK
                     SGRFSFNVTGGRCEACHGDGLVKIEMQFLPDVYVTCEVCHGQRYNQEALEIHYKGKNI
                     YEILEMTIEEALEFFTNQPAIKDKLAALNEVGLGYIKLGQPATTLSGGEAQRVKLATE
                     LARKSTGKTLYILDEPTTGLHFADIHKLLEVLQQLVDKGNTVIVIEHNLDVIKTADWL
                     IDLGPLGGQKGGQIIAEGTPLEIAANPQSFTGQYLKKSCQKVNKILTIPLKLPKLIKL
                     NQISAIFISRKLL"
     gene            complement(12393..12469)
                     /locus_tag="PKV49_00535"
     tRNA            complement(12393..12469)
                     /locus_tag="PKV49_00535"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12433..12435),aa:Pro,seq:cgg)
     gene            complement(12769..14511)
                     /locus_tag="PKV49_00540"
     CDS             complement(12769..14511)
                     /locus_tag="PKV49_00540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPL22143.1"
                     /translation="MSPKIIWPIIVLLAALTVGLVVNYYQSCDKNQARPNNLTPNNQA
                     RNEKQFLDFLSEKKQLPQASQEVSMIAVGDISYSRAVERMVKRQNNINYPFLKIRDYL
                     KNADLVFGNLETPITPGAEIPDFAMIFRSNPGTEQALKQAGLSIVSLANNHTPNFGEK
                     GLNNTFNYLNAVGIKYVGAGLNDQEAYQPIYLDQQGIKLAFLAYNDTDVVPNHYEASA
                     NRAGTAFMRIDKLSEAVQAAKQNADLVIVSMHSGVEYVEKPNNSQINFAHAAIDAGAD
                     LVIGHHPHVVQTLEKYKGKFIFYSLGNFIFDQPQSAETKEGLAIKIYFTKNGLSKISL
                     LPVVMKNLAQPEAADKDKAAKILQRLDFPLANQDVYMWNQHNFEKTVRKIIYNDAFNN
                     NKIITKQERADLDNNSVSENYNLANGQLTITENEKIIWQSPSDWWIDNFVLADSNNDG
                     ITEINLSLWKSGSFGASKPFWIEENDVSVKNHFFVLGFSDGSIKQLWGSSNLPEPNCE
                     FKIADVDNDGKNDLVVLEGDYGQKPQCIGNYLAVWKWNGWGFTNEWRSAKGNFANLDI
                     EKHDGKNYIIVDTP"
     gene            complement(14517..16826)
                     /locus_tag="PKV49_00545"
     CDS             complement(14517..16826)
                     /locus_tag="PKV49_00545"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HPL22144.1"
                     /translation="MAKHNFNQPMPDSPDGSPIPPTTSKPKPRKTFWIILGILDIVVI
                     VTLVIYLVSPKSEQNIIKEVFETIYQPTVAFASKFATYEDIPVNINPKVPAYSVDPNL
                     SNITNANDFIFSDAAKNLLIKNAFVVRPSYHNEFFSLYETNRYDYIPSFITTDSMLHN
                     YHLMFDFLLKQLEEQKLAAELKQLNANMLAESLNQYNSLKGTEWENAAKRNVGFFAVG
                     SKLLDSSVNIPSIVASEVNQELALIAAHQDIEKSPVMNIGSEQNTIIDTPQGQQSLEA
                     FKEDYSQYIPRGHYDKTEQLKAYFKSMMWYGRLTFRMKNNDEVKSALLITLALNQENN
                     QNSWNKIYEPVNFFVGKSDDITYYQFKDLVEQVYGAKMTIQSIPGDNNKFASLIKATE
                     SLEPPQINSIPILNSTIQPDREEEVKGFRFMGQRFTIDAAIFQRLVVREVGPKAESCA
                     NAPFRNGRMLPKGLDIPATMGSDEALNILKTQGETQYACYPKNMSTMQTYLAALPTEN
                     WTQNLYWGWLHQLRPLLDKKTSGYPIFMQNNAWVRKELNTFLGSWSQLKHDTILYAKQ
                     VYAELGGDGSEEKDDRGYVEPNPYVYARLASLLRMTKEGLETRGLLTTSMKDNLNKME
                     QLAMSLKTISEKELNNEKLTDDEYELIRSFGGQLEHFWLEINKDEPQYKELGQKHFLD
                     QNPAAIIADVATDPNGQILEEGIGKIFEIYVVVPIDGQLRIAKGGVYSYYEFTWPMAD
                     RLTDEKWRELLNSETAPALPSWTNAFVAE"
     gene            complement(16994..17067)
                     /locus_tag="PKV49_00550"
     tRNA            complement(16994..17067)
                     /locus_tag="PKV49_00550"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17033..17035),aa:Ala,seq:cgc)
     gene            complement(17157..18647)
                     /locus_tag="PKV49_00555"
     CDS             complement(17157..18647)
                     /locus_tag="PKV49_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPL22145.1"
                     /translation="MTKPDSLPQTSKSNQAGKQCLSSLPDFVQQALIILQQAGFEAFI
                     VGGCVRDLLMGHQPKDWDITTNARPTDILAALPDSKYKNSFGTVIWPIYLTDSTEVLE
                     ITTYRSESDYDDHRHPSEIIFEDKLENDLSRRDFTINAMAMNQNGDIIDYYGGQKDLQ
                     QGIIRTVGEPADRFKEDALRLLRAVRFSNRFNFIIEPKTERAMIKLAGSIKFIANERI
                     RDELIKILAGPQPYEGIKKLHETKLLQYILPELEEGDGVAQNHHHIYTVFDHNLLSLK
                     HCPSQDYLVRLAALLHDVGKPRAKKTINGQTTFYNHEYISAKLTQKIMTRLRFSNQEI
                     NRVVNLVRNHMFYYNVGEVTEASVRRLIAKTGEQNLADLIDLRIADRLGSGTAKAKPY
                     KLRHLEYMFDKVRHDPVSVKMLKINGTDLMQKFGLEPGPQIGAILDVILADVINNPQL
                     NEREVLLKLAQPLINLNLAELRQRAKTVIAEERSLEDQQLRQQHRV"
     gene            complement(18640..20139)
                     /locus_tag="PKV49_00560"
     CDS             complement(18640..20139)
                     /locus_tag="PKV49_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22146.1"
                     /translation="MRKIIISGGIILSAILSVYFVISPASAASPFAGKILLAVEGHGE
                     AWYINPTDEKKYYLGRPADAFRIMRGLAIGISNADFSLLENKRLASAKVKRLSGHILL
                     KPQDQGKAYYFEPSQRKLYYLGRPADAFRIMKETAVGATNEHIDPIPTGIINTAAKTN
                     NPNTKLWRWRYAGRDYSIQLDLSTKLYDYYRQQSKVFTYTGDNLPSNWRNQYYGQFLN
                     NASQDNIIRQITEQLQKQATTNQLTADQTAELAAAFIQSIPYDQNRSDLIQQPNNQIT
                     PNYPYETLYLNRGVCTDKALLAWLIFRKLGYGVALLSYPEANHMAVGIACPQQAANYS
                     TGYCYIETTQFYHIGLLPSSINGQAASSSSVTLPSWQPSGNLAEPLIYQATAGKNYGG
                     LSNLQATLSQISEQKIASDQSRQIINQQQVELSRRQTELQQQQQQLEQYRNSNQINQY
                     NQLVPIYNQAVQAYEQFRQYYNQLIEQFNQTVARYNQLLNSLQLSQSND"
     gene            20218..20508
                     /locus_tag="PKV49_00565"
     CDS             20218..20508
                     /locus_tag="PKV49_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPL22147.1"
                     /translation="MVNQPTAQDLVAWRSMIANRQKWQLMVKVTANCPHCGWRGQLAD
                     GRWKIGLNEPAVDNRANQALLVWLSKELNCPVSRLAISHGRTSGFKTIVAIN"
     gene            20512..21057
                     /locus_tag="PKV49_00570"
     CDS             20512..21057
                     /locus_tag="PKV49_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPL22148.1"
                     /translation="MTKKHKPKLLLHICCGPCATVAANRLMEDYQVVLLFYNPNIEPI
                     AEWQERLAAAQKLAQIYGLSLIVDDTDFEDWQTMAKPLAIEPERGRRCQLCYEWRLRR
                     TAQLADQESCAYFATSLTVSPYKDREAINKIGLIAAGVTRANYLPTDFQQADGYRQSI
                     KLSKQYGLYRQKYCGCRFSRQ"
     gene            21102..22145
                     /locus_tag="PKV49_00575"
     CDS             21102..22145
                     /locus_tag="PKV49_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPL22149.1"
                     /translation="MDLKIYFEQAIARRASDVHLVGGSCPYLRIDGQLIKISDEIIDA
                     DQLAKAVKGFLDERLIEKLEDNKELDISQAIAGGHFRINLHYQRGVIGLTARLISSDI
                     PNPEDIGLDKNLQRLAFLNDGLVLVVGACGSGKSTTLASLLGIVNRERQGKIVTIEEP
                     IEYIFKNERCLIEQREIGIDTNDFAVALKYVLRQDPNVIMVGEMRDNETMEAALTAAE
                     TGHLVFSTLHTSTAVEALSRIVSSFPAYQQGQIATKLSLCLRAVISQRLLPATAGGRV
                     AVREIMFNTPAIANLLRSQKLEQIPSIIQTSSREGMISFNRAVEQLLMTKQISPEVAR
                     AYQRGDSGASYWR"
     gene            22165..22899
                     /locus_tag="PKV49_00580"
     CDS             22165..22899
                     /locus_tag="PKV49_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPL22150.1"
                     /translation="MWIIFLVIIVALLLASLRQINQYQRGVLFTMGRFSQIMEPGWRL
                     VIPVFQSFQKVDMRVKAVDVPDQRAITKDNVSVTVNAVLYYKVFDAAKAVIEVENFYY
                     AISQYAQTTMRNIVGEVTLDELLTNREKIAERIREIVDVETDAWGLKVNNVELKDVSL
                     PPTMERTIAKQAEAEREKRAVIINSEGEMAAAENVAKAAQILSAIPGALHLRTLQSIN
                     DISSDQSNTIVFAVPVEALRALDGFK"
     gene            23083..23664
                     /locus_tag="PKV49_00585"
     CDS             23083..23664
                     /locus_tag="PKV49_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22151.1"
                     /translation="MVWLIPTLNLLAWCGFLTIVNSSFIRQLLILTTAAIQLAYWRRG
                     WTAWLEQKPPAAWRRLVNAINLLTIWLAAATLYGWQAFLAWPIWWSWLWWLILSGLIL
                     WLDYQTLALPLSTHWPVVLTQWLIGGQIFLAVYFLPSSHLVLAYLLLIAYYVVSQIGR
                     GSLLKTNTARRLRYQFLILIILLAVVLLTAKWF"
     gene            23681..24766
                     /locus_tag="PKV49_00590"
     CDS             23681..24766
                     /locus_tag="PKV49_00590"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HPL22152.1"
                     /translation="MSSTLIRKKISLSLIWWILAIVVNLILALVMVKYFFAIEQANLL
                     TSATLSGQNAIGWPMAINQSSTTKRPAAIEYNEAASLQPSLSLSLVKVFRQADRITSA
                     PIKQDNLYNLNQPLAGGWVATSDGWVVSSADLDNWSDLAVVAGNRKVYKIDQKTSDPL
                     TGLFFYHLAGAANLSPVKLGEVGWSRPGQLMYNLDNLGGLKAVWLERVTTDQNNNIIS
                     SDILWYRWTFSSVWPSSAIFNGQGDLFGLVDSQGKIIPANYIKSGLNSLLSMGNFKRV
                     VLGIEYFDLNRSSTERSGLLVNQVTAGSSADKAGLKAGDIILSIDEINLDGQIAINEI
                     LQGYKTGNRASLIVQRAGQNRQLNCQF"
     gene            24771..25952
                     /locus_tag="PKV49_00595"
     CDS             24771..25952
                     /locus_tag="PKV49_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPL22153.1"
                     /translation="MFSRTISAMPNLSGRQVFLRLDLNVPLNGQEIVEDYKIRAALPS
                     IKALLDHGAKIIIASHLGEPLVKGKLIVGAKTALTIKPIAARLAQLLDRPVKFLPGQN
                     LAVLRQKISRATENVIVLDNLRFWPGELENNRDFAKELASLADFYVNDAFAVSHRQQA
                     SVAAIKDFLPSFAGILLEQEVVNLSKALNPKQPLIVVLGGAKVGSKVHLIRRLGRKAS
                     KVLLGGGLANNFLAAQGYQVGQSLVDKESIDLARRLIKELGKKLVLPIDVIVADSSSQ
                     PVLRLINQVGDQDRILDIGPATIQEFSRQIKMGKTIVWNGPLGQFENEHYKNGTLFIA
                     REIAWQSRGAAFGLVGGGETVAALQMTQMAGYIDWVSTAGGAMLAFLGGEPMPGLKNI
                     I"
     gene            25974..27242
                     /gene="eno"
                     /locus_tag="PKV49_00600"
     CDS             25974..27242
                     /gene="eno"
                     /locus_tag="PKV49_00600"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010631933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPL22154.1"
                     /translation="MAKIVQLTAREILDSRGNPTVEAKVTLDNGLVAKASVPSGASTG
                     VHEAHELRDGDKNRYGGKGVLQAVANIKDVIAPKIIGQEIIDLPEIDRIMIDLDGTAN
                     KEKLGANAILAVSMACARAGAMATGQELYAYLASIYDFATDNYRLPTPSFNIFNGGQH
                     ADTNVDFQEFMILPLINSTVAEKIRLGAEVFHALGKVLQEAGLATNLGNEGGYAPKME
                     YSEQVMEMIVQAMSRAGYQPKEQVGFGLDVGSSELYDEATGYYTFSLDKWQFTSAELL
                     AKYREWSEKYPIISIEDGLAEDDWSGWQNLTAELGDRMLLIGDDLFVTQTARLRQGIA
                     SQAANAILIKLNQVGTVTETIECIKVAQQNNYKIMISHRSGETTDDFIADLAVAVGAD
                     YIKTGSTARGERLAKYNRLMEIELQLAGNF"
     gene            27249..28166
                     /locus_tag="PKV49_00605"
     CDS             27249..28166
                     /locus_tag="PKV49_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22155.1"
                     /translation="MPVQTDKINNDSAASSVVLVLLDGWGVAPPGPGNLLTNLALPNL
                     SDYLQQYPLAVLATPLIRNGSAKEAYCQIGSGQDSSLVPGLGGVADQSGLAELLSSAG
                     RRQAYFTETEKLPLVVECLAGGRQDPEIEYRVIATPLNNDYNEQVLAASHQLIDQAVS
                     YYRHYRPDFLTINLAAWSSVARYGTVESIAKAMVAMDKLLPKLLNPIIQTGATILLTS
                     AYGRLESLIDPLTEKVNFGPSNNPVPFLIIGRLWPGQSLGETEIASNDLSVLPLSGSL
                     RSIAPTILALLGVERPSYLEKPLLPININ"
     gene            28173..29219
                     /locus_tag="PKV49_00610"
     CDS             28173..29219
                     /locus_tag="PKV49_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPL22156.1"
                     /translation="MAVLLLNAGSTSLKYQLFSDGGEVIKRGGSSRVKDFDQAFSQII
                     EELEFIGEITAIGHRVVHGGDHYQRPTIINDQVIDYLESIVNLAPLHLPFNLLGVRLA
                     AEHWPNLPQVAVFDTAFFANLPTVAKTYALPLDLATEKHWHRYGFHGLSHAYLTHAAA
                     DELNKKLEQVNLIICHLGGGSSISAIQAGKAIDISLGWSPTAGLPMMTRCGDLDPGLL
                     LTIIEDLPGPVNEQKINQVRDLLNKQSGLCGLCGYDDFLDILTARQHGETRAAAAFDY
                     FVYRLVHYIGAYWAILGGKVDAIVLSGAIGAGSLDLRQALANKLSGWCSASLLAMPTN
                     EELAMFREIKKLGL"
     gene            29223..30848
                     /gene="murJ"
                     /locus_tag="PKV49_00615"
     CDS             29223..30848
                     /gene="murJ"
                     /locus_tag="PKV49_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPL22157.1"
                     /translation="MALKKLWYGSINNIGWAAVVVGVASFISRFLGVFRDHILAGQFG
                     AGDELDIYYAAFRVPDLIFNLLVLGALSAGFIPIFVSLLNNYDEPANNHDAWRFANLV
                     ISSLIVILLILSVVGIILAPQLLRLLTPGFSADKLATTVKLSRLMFLSPLLLGISGVV
                     GGILQSFKNFFAYSLAPIFYNLGIITGALFLVPRFGLMGLAWGVVIGAALHLLVQLPT
                     LWRLGFRPKPLLDWHDQALKQIGRIMIPRTMGLAISQLNLVVMTILASALTSGSLAIF
                     NLANNLQSFPVGIFGLSFAIAAFPTISALVDQPDKLKANLTVTIRQTLFLVIPATIII
                     LALRAQIIRVILGSGRFDWQDTILTMRTLSYFGLSIFAQSLTAILVRVFYAYKDSATP
                     FWISLLAAVVNIGLAWPWSRQLGVAGLALAFSVSNIVNFILLWWALRRKGNILDNLTI
                     GIGSLKMVLAAAIAWPAIFAAKRLIAPLVDMDTFWGITSQLIGAATVGLAVYLFICLL
                     LRCPEAKQLWQIVNRRLPGGHKTVVDADEARGI"
     gene            31122..31520
                     /locus_tag="PKV49_00620"
                     /pseudo
     CDS             31122..31520
                     /locus_tag="PKV49_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            31708..31962
                     /locus_tag="PKV49_00625"
     CDS             31708..31962
                     /locus_tag="PKV49_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22158.1"
                     /translation="MEQFAQAAKINKWEVVKLVAARQHIWRCARDLKKVLPEICLDKI
                     PVKVAHCPDIQAWVTSPLRWWVREIIIRLLPWGLYRRVCG"
     gene            32229..34013
                     /gene="lepA"
                     /locus_tag="PKV49_00630"
     CDS             32229..34013
                     /gene="lepA"
                     /locus_tag="PKV49_00630"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPL22159.1"
                     /translation="MQNIRNFCIIAHIDHGKSTLADRLLEFTGTIDPRKMTNQVLDRL
                     DLERERGITIKLTPVRMKYQGYELNLIDTPGHVDFSYEVSRSLAAVEGAILLVDATQG
                     IQAQTLANLYLAIEQDLAIIPVINKIDLPAADVDKTKAEIVKLLGCSAEEIICASGKT
                     GTGVPDILERVIKVVPAPSGQPDQPLRALIFDSNYDEYRGVVAYIRVVDGAIKKADKI
                     NLMATQAESEALDIGYFDPNYHSSGQLNAGEIGYIITNFREVGECRVGDTVTWSRTIG
                     QTVPLKGYTEVRPMVFAGIFPKEGNEFEDLREAMGRLKLNDAALVYEPEQSQALGFGF
                     RCGFLGLLHLEIFQERLRREFNLDLIVTVPSVAYRLRRKNSSEIEIIRSPQQLPSAGD
                     IDLIEEPWVNLDIVTPKDYLGAVMKLAQEKRGIYRNTEYLDETRVVLHYEMPMSMILT
                     DFYDKLKSSTSGYGSINYEILDYRQADVVRLDILVAEDMIESLSMIVYRDEAFRRGKE
                     VVTTLKNTLPRQMFVIKLQAAVGGQIVAGERISAFRKDVTAKLYGGDVTRKRKLLEKQ
                     RQGKKKMMAMGRGSVDIPSETFVKLLKR"
     gene            34030..34155
                     /locus_tag="PKV49_00635"
     CDS             34030..34155
                     /locus_tag="PKV49_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22160.1"
                     /translation="MSNKRNKKKKITKRQKIRIAWIIISAIVIISMVAGTIIMSL"
     gene            34157..35881
                     /gene="recJ"
                     /locus_tag="PKV49_00640"
     CDS             34157..35881
                     /gene="recJ"
                     /locus_tag="PKV49_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPL22161.1"
                     /translation="MAAERLWQVAPVLVNRNPKLAELKLPEIIEQILYNRGLTDPMAV
                     RQFLGLESFDCSPGRLSQLEAAVELIVEHIKAGHLMVVYGDYDADGVTASTILLETLT
                     TLKAKARIWLPDRITEGYGLNKPAIDELAAANAKLIITVDTGIRNKEEIAYAKTKGLD
                     IIVTDHHRESPDPADWPDCLMINPTMAKEDYPCKFLAGVGVAFKMVQALLRRSTLDSS
                     QKQILERRCLDLVALGTIADCVPLAGENRLLVKQGIEVLNEWRRVGLRELARVAQITG
                     EVNEWNISWQLTPRINVAGRLDHANTAYRLLTTTDQAEAAQLAEDLNQKNLDRQQKTQ
                     EIVDFCLNDLLELQAEDNLLIFISPRQEPWPEGIIGLVAGRLSEKTAKPVLVITKSEN
                     GYKGSGRSNVENFSLIAQLEEVASDLDRYGGHAGACGFSLTVQQLPNFLTKIKKLAAA
                     KLTGLDLRPKLFIDGELPADQVDLSLAEWLEKLAPFGQGNQQPRFVAYRQTIRDINTM
                     GANKQHIKLRLGNYWALAFGQAEDYLAQDLRPGRQIDLVYSLDINRWQGQAEPQLKII
                     DLRPSTDK"
     gene            35897..36325
                     /locus_tag="PKV49_00645"
     CDS             35897..36325
                     /locus_tag="PKV49_00645"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPL22162.1"
                     /translation="MVKKFVHQYLISYTDGGARGNPGPAGIGVVLQNKDGETIGEWSE
                     FLGIATNNQAEYKALLLALKQAVALGAITLECRLDSELVVKQLKGEYKVKHPELKPLF
                     EQAKSLISQIKNVSFKHIPRELNKQADQLANRAMDQGGDF"
     gene            36325..36401
                     /locus_tag="PKV49_00650"
     tRNA            36325..36401
                     /locus_tag="PKV49_00650"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36359..36361,aa:Val,seq:cac)
     gene            36519..36898
                     /gene="rnpB"
                     /locus_tag="PKV49_00655"
     ncRNA           36519..36898
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKV49_00655"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            36914..38326
                     /locus_tag="PKV49_00660"
     CDS             36914..38326
                     /locus_tag="PKV49_00660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPL22163.1"
                     /translation="MKRSELFFSFILVPVDFLMVFLAGISAYHLRASEMATGIRPILY
                     NLPFGEFLWSLVVVAAAWLIIFAIAGLYRARGIKRFEELSQVFFACFAGFGLIVIYIF
                     FFREWFSSRFIILVGWLLAWAYVSLARLIVCFIQKQLFRYGIGVHRVVIVGRGASADK
                     LIKEWFSHRSRGYQVVKHYDDFSLEIAQELADYLQVKEIDEIIQADPNLSKAEVVRLY
                     DFADEHHLTFKYVADLLDTKVLKTEVDEVAGIPVVEVKKTTLDGWGRIIKRLFDIVVS
                     GILIIILSPVYLLTAIAIKLDSAGPVFFSRLDDGSPLCRIGQAGRKFRYLKFRSMAPG
                     TDSLRYTELADRNIRDDGPMVKIKDDPRITRVGKFIRRFSIDELPELFLVFIGSMSLV
                     GPRPHLPEEVAKYQRHHKKVLTIKPGITGLAQVSGRSDLSFEEEVKLDVYYIENWSLA
                     RDIRILLRTPMAVLRHRQAA"
     gene            38329..39435
                     /locus_tag="PKV49_00665"
     CDS             38329..39435
                     /locus_tag="PKV49_00665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22164.1"
                     /translation="MKIALIHDHLAQDGGAEKVLKVLAEMYPQADIYTLLYEQKHADK
                     YFSGRTIKTSFIQRIPGGVKHYRWFLPWMPMAVEFFDLSDYDLVISDASAFAKGVITG
                     PEVPHICYCHTPTRYLWDYTHRYIAELRYNKFLKKIVSLFLNYLRLWDRQSADRVDYF
                     VANSKLVQKRIKKYYGRDSQVVYPPVDVDSFKVVDQPSDYFLIGGRVVVYKRFDLVVE
                     AFKELGWPIKIFGDGVDLGRLKKMAAGASNIEFLGRVDEAKKIELYQNCRAFLNPQEE
                     DFGITVVEAMACGRPVIAYAKGGALETVVDGQTGVFFQEQTVKALVSALRRFDQLTFN
                     PATIRQRAELFSRSNFEQQFKDVVNKSLTDFQQR"
     gene            39441..40589
                     /locus_tag="PKV49_00670"
     CDS             39441..40589
                     /locus_tag="PKV49_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPL22165.1"
                     /translation="MRIAVDARPLLAKQISGIPEYTGRLIDALVTAHPEADWRLFYSG
                     WHQRPDNWPALKEKPNISWCRLKCPNKLINSAAWLFDRPRLDKYCSADVWLLPHFNFT
                     PLTGQIPTILTIHDLSFLRQPEFFSWRRRFWHASLHLPRLINKVNRIVAISENTKRDL
                     IELMAVPEKKITVIYSAANPAFRPIMSDDQRLTNCRQQYNLPKRFILSLATCEPRKNL
                     VNIIRAYDNLRQRCPSLADCSLVIAGGQGWKQGAARIAWRQAANRQDIYWLGYLPADD
                     LPIVYNLASLIVYPSFYEGFGLPVLEAMACGRPVITAATTALPEVAGTAALLIDPADS
                     QALSRAMEQILTNRQLAEQLAQAGRQQASQFSWSKTAEEYWKLLRFSL"
     gene            40657..44205
                     /locus_tag="PKV49_00675"
     CDS             40657..44205
                     /locus_tag="PKV49_00675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPL22166.1"
                     /translation="MSNQPFVHLHVHSHYSLLDGMGKIDELVEAAKADGATAVALTDH
                     GVMYGVVEFYQKCQKAGIKPIIGVEAYLTPGSRHDKNSQIERSYHLILLAKDQVGYNN
                     LLKLVSIAHLEGFYYKPRIDWECLQKYSQGLIAMSACLAGEIPRLIMSGKKEQAKKRA
                     LEYNQLFGQGNYYLELQDHPHLPEQEKVNQELIKLSQATGIPLVATNDVHYIRASDRD
                     VQDVLLCLQTKHKKNDAGRMNMLEDDYSFRPSAEMAAAFAAVPEAINNTVKIADQCNL
                     ELAMGQVLLPRFTVPNDLTAQDYLRQLCLDGLVKRYGQTYEEIEPLYRERMDYELEII
                     AKMGFPDYFLIVADFINWAKNNAIVVGPGRGSAAGSLVCYLIGITELCPIAYDLLFER
                     FLNPDRISMPDIDTDFADIRREDVIEYVSEKYGRDHVSQIITFGTMAARAAIRDVGRV
                     LDASYDFCDKLAKMVPMFMKLPEALEQVAELKQIYQQDPQAKQILDYAQRLEGCARHT
                     STHACGVLITKDPLTEYTPIQYASSTDRTVVCQYSLHPVEDLGLLKMDFLGLKNLTII
                     ESAIRIIKATKGIEIDINQIPLDDQETFGLFQRGETTGVFQFESSGMKRYLRDLKPTN
                     LEDIIAMVALYRPGPMEWIPDYIAGKHGRKKPQYLHPKLEPILAKTYGVAIYQEQVMQ
                     IARSLAGFTMGQADVLRKAMGKKIAKLLAEQKEKFIAGCVNNGVSQTLAEKVFSFIEP
                     FAGYGFNRSHAACYAMVAYQTAYLKAKYPTAFMAALLAADQQNSDRVAIEIDECRHMG
                     IEVCQPDINQSFDSFTVVNEALVDKPTIRFSLLAIKNVGEHIAEVIKDERKTNGHYQD
                     IADFLRRITDKDLNRKSLESLIKAGAFDAWHDRQQLVDNLDILLKYNKDSNQNRLSGQ
                     NSLFAGVSGVEHQPLVLRSAPPVNRQTKLGWERELLGLYITEHPFVDFKKQLGSALTP
                     IKDLAALNNEQNVTIGGMVVSSKKIMTRANQSMLFVKLEDETGSTEVIVFPTLLATSG
                     EVWQDGQAILCQGTISDKDNERKILADKAIYLTLDNLTDQLATFRELPGQKRRAKSGN
                     WSGPVVVNSADRSATKETANSLAEQFDLILNQEFDQDQLVQLKEILLKYPGPSQVYLE
                     INLNNNKQRIATGFKVNRQPKLLAELMAKFAGMVKF"
     gene            44243..45727
                     /locus_tag="PKV49_00680"
     CDS             44243..45727
                     /locus_tag="PKV49_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22167.1"
                     /translation="MPVKKTKTTAAKAGSGSTKPVARRSAGRPPKARPARPSVRRSTK
                     AQPAAAKKVVRRQPPVQPKVVVVTNQPLITNEEMAVWQTVRQENDQPEQLADNAALSS
                     NPPSQIPVDEPFINQWTTVNSYVPTESDQRFGRLYRRLALFFIGLITIVVMVMFYYSL
                     IRVKITIYPKSDQPARQQTIVLTDTVPATDGAVPAKFLQINSEYRQTFRATGQSQTTG
                     SGKLVGQVTLVNTTAKDQPLRATTRLLTANGVLYRLRNFVTVPAGGQIIAEAYADQVK
                     TENAVAAATKFTIPGLWSGLQDKIYAVANKNIDYQSDSQAIVLATDLEQAISQAKQAA
                     AAQAKNQLDQQAGGEALVYWLDDSQWSISSDAQAGDQKAEFTITVKAAAGGAIVSAEA
                     IKEVLNQADDAIKINEQSLTYQLSAWQPQQHQAQLNVSYKVELVSDQADQLVDKRALV
                     GLNRQQLDTYLSGQPNVASYKVIWQPGFLSKVPGTDKIQLQLGR"
     gene            complement(45802..47859)
                     /gene="gyrB"
                     /locus_tag="PKV49_00685"
     CDS             complement(45802..47859)
                     /gene="gyrB"
                     /locus_tag="PKV49_00685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPL22168.1"
                     /translation="MNKNFQQATAEYSAEAITVLEGLEPVRKRPGMYIGSTSATGLHH
                     LIWEVADNCFDEVMANYADSVEITLLPDGLVEVADNGRGIPVDIHKTTGVSALETVMT
                     KLHAGGKFGGSGYKISGGLHGVGVSVVNALSSYLKATVYRDGAIWEQEYQCGKPSGPT
                     KKVGKANNNQHGTTITFRPDETIFDTTEFKWGTILDHFRQQTYLTKGVTVKLHDRRPG
                     HRSKDYVFYFEGGIKSYIKSLNRNNQVKNETVFYAEKELDNVKVELALQYNDGYNETV
                     LAFANNIINMEGGTHLIGFRSAQTRVLNNYARSKNILKEKDDNLTGDDVREGLVAIIS
                     VKLPSPQFEGQTKGKLGNAEIKGYVEQVCSETLSTFLEEHPKEAEQIIGKCLLSAQAR
                     IAARTARTAILRKSALDSATLPGKLADCSSRKAEECELFIVEGDSAGGSAKQGRDRRF
                     QAILPLRGKILNVERARLDKMLENNEIKNMVIALGTNIDDQFDISRLRYHRIVIMTDA
                     DVDGAHIRTLLLTLFFRHFNPLVTNGHLYIAQPPLYQIKKGTTVRYAYSDEEKDKIVK
                     ELGGTSAVVEINDNQNEDDNSEQNEPSIDNDSPEQNNKPGKNAKTPTKLHIQRYKGLG
                     EMNPEQLWSTTMNPENRVLRQVEVEDAAMANEIFEMLMGNEVAPRKNFIQTYAKKVTN
                     LDI"
     gene            48033..50177
                     /locus_tag="PKV49_00690"
     CDS             48033..50177
                     /locus_tag="PKV49_00690"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HPL22169.1"
                     /translation="MKKALFSNNLPEDTTISQSLAKRHSSSGNWLWIASAILSVVFII
                     IISRLIVVWGQQAKVKNQSQSAINPNQITEQPTLQFGQDADPGTIVRQDPDFRADQLV
                     FSQIYQAPTTSWSTAILANQAQAVKLPLNVKNQAANYYEIRRRYNLEPLIKKINEHGS
                     VIIDNPFGQPEQDFFSFYQAARQDKWPVIITNDAIFYYYQNNLKEVYKALEAGLYDGL
                     WQISLNLFDSANRRYQAAVSQAVDGNDPLLEAYRLNVQYWAVTLNLLVPKNNQINYDN
                     LAAATTAFQTGDDQIYKMPTLSSDLKQEVEQEVALIQAADGPNLSPIFGQLFDYRLFK
                     PDASYQSGGRLANFSLASQWLGRPWPVYYRNQDCPDCQLDVDDWRMDFLAANILARDL
                     SSNQEVKNYWAKVYKTISYFAGLRDDLTYIHYVKAQQDVLAGQSLEQVARLDASARAA
                     VLDKLQAAILKNSFSLAEGGHDRRQLKERPNIGLRVLQERYWPGQNINWTKIGSQLDN
                     YLNQQSAKDWQANQFWSSVGILKDWLAKILVVDKSWIRPAWWREQQLALAKFYLINWQ
                     LPADQWALVLPSSGLAQNTVTADSLIAIDTSGLPAIERQIATAKMLRLALAKLQVSNL
                     LSEQRLNETIDTLQIIRDLAIKQATGQAWTKDDKTAAGNFFQRSLLKAGRKTISDKAN
                     DSESSQQRLRARLEIRNMAGQTMILIGPVMMP"
     gene            50189..51073
                     /locus_tag="PKV49_00695"
     CDS             50189..51073
                     /locus_tag="PKV49_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22170.1"
                     /translation="MPIKKTAPTNNPLPDRLGDGDISLKQLSFGLWYLRHRRLFYSLA
                     IGLLVVLVIAGWIYGFYGLGRYFIFDRSRDRQSLLDIAQSGVNLLAGRTNQPLSISEV
                     KIFGNNPSDLLAEIYNPNNRTAVYFKYAFIANGQPIVQREGFVLPGQRKYLVATGQQV
                     PAGAAVQLAITQTFWQKLDARQIPDWPAFLANHDQLVISDRQISFAGLGDQAVTQVKF
                     RLDNKTAYGYWSIPILMLVYQGDNLIGVNELVLSKVKSGESRMVNFSWPGLSGADHLE
                     VVTMVDYLNTDNYLPLED"
     gene            51077..52177
                     /locus_tag="PKV49_00700"
     CDS             51077..52177
                     /locus_tag="PKV49_00700"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPL22171.1"
                     /translation="MLGKLRQYWRRNDWLLTVAMLLLLAVGLTEIYGVSLGRGGVSLL
                     AFRKQLIFSLIGLIGFIALSLSDYHRWRGYVNYFYGLAIVLLVAVLVFGSPINGTRGW
                     FNILGLGLQPVELVKIILIIFLASYFSSRTIRIDQWKIFLKSGLATLVLMILVLLQPD
                     FGSALLLGSIWLILILVIGFDRKYLYWLAGLAIIGIVVGWLFLFTNIQKSRIRSFFNP
                     NQQTFSQSYNVNQAMIAVGSGRILGRGVGFGSQTQLKFLPEAQTDFIFAVIAEERGLL
                     GAGLVVLLFAVIIYRALAVLPKIKDDFGCFVVVGAIGLLAIEVFVNLAMNIGLLPVVG
                     IALPLVSYGGSSLIASLLLLGLVNSVIIRTRS"
     gene            52187..52942
                     /locus_tag="PKV49_00705"
     CDS             52187..52942
                     /locus_tag="PKV49_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPL22172.1"
                     /translation="MNTKDLDRLIDRQLTDKLEKLNLRNDSIYPIPDSIQHIWQVFID
                     GQPVEHYAHLAAQSKYGVMNLGISPDLGQHVTFQEPGGGGSVIIPFVIIQKQRVIELK
                     RLSKQQDYQVYAGLIEQQRDRQGGLVWNVPRGYVEQGDNHDQTAVKELTEETGLAGQP
                     FLLPGQSLNPNSSYFETGKDNGVLIYAINLGPDRAVWRDSQVVLLDNPQPTEIHEKIG
                     QCRFFPLAELSQLGDMFSVAAEARLRYYLDNKL"
     gene            52985..53875
                     /locus_tag="PKV49_00710"
     CDS             52985..53875
                     /locus_tag="PKV49_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPL22173.1"
                     /translation="MKDAQKLTPSAFVDLAKLLAGQLRLRYEELDSDHGRSLTNSINQ
                     FGDQTLIADWELEESVIKFWQSTGCQIGWIAEEHPMLFNKNEVDFIGYLDGLDGSSVY
                     KTNEDGRCGTMMSIYQAEDQTFAGYVGSLVMDYGSHKFGGNCSYIAAKGLGAWADPVT
                     RGDYIQINVVPDDQLTRQMRRKLLAYADLYFESVRRATKKMTGIKFRYLQASMPYYVD
                     LAQGYACLVVECTRKHSLEIASAYPLVHEAGGVIVDLASGQDLGGLDRSLAEASEQII
                     VSAPNLKIVDEITSQMSASY"
     gene            53990..54712
                     /locus_tag="PKV49_00715"
     CDS             53990..54712
                     /locus_tag="PKV49_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPL22174.1"
                     /translation="MLDKLKELDLITPSILPMPKGVVWSWQLYINGEPVDMSFVQHIA
                     IKSRFGTVNFGADERAGYNSVAFEEAGRGGAVIVPFFCLKDGQVVAPNQLYDELYIGV
                     VEQYRNKQGGMVLNVPRGFVKQGGEPHEAAAKREIFEETGLIAEPFLLPGHNLNPNSA
                     FFETGEDGGCRIYAIVLSPEQVAIDDDGIHLITDNIPEDSREIIGKCRFIPAHEVSQL
                     GDMFSVAAESRLINYLISLDTN"
     gene            54843..55496
                     /locus_tag="PKV49_00720"
     CDS             54843..55496
                     /locus_tag="PKV49_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPL22175.1"
                     /translation="MKSELKLAASTRQPGRQALSQLRSHGFIPAVVYGPKTDNLHIQI
                     NKAEALKIYNQAGGSSLITLDIDGQDQRQVIIKDIAQTRVKDQLIHMDFFQVDMAEKV
                     TTEIELNFIGEAPAVKQLGGVVVKNFDAVEIECLPKDLTNKLDVDLSGLVDFTTHISF
                     ADLKLPKGVVLSDKHEMNEIIVAVEEPRAVAVENAAAEEAATTETKTESKPEAEEKA"
     gene            complement(55544..56443)
                     /locus_tag="PKV49_00725"
     CDS             complement(55544..56443)
                     /locus_tag="PKV49_00725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HPL22176.1"
                     /translation="MLNSWLKNPTAKRPTISQAQDWLSQRWPLGQAQPALISWLLSFI
                     LQQPASYLITCPDQLLTARQWRQLNQALIKLTNGYPLAYITGRQDFYSRQFIVSPAVL
                     IPRPESECLVDIALELRSKLTESSAWLDIGTGSGCLIITLAQELNNADYYGGSDISAS
                     ALKIAKKNANLHNTKIIWQHGSLLKPWLSTNFKPAQQIFLLANLPYLKPKNYHEHYTE
                     LRHEPKLALVNSHNGLGLFKKLAQELPLFIQHHPNQTIHLLTESDLQQVSQLQNQLAV
                     GGLNWQQSFPDLTGRQRFGWWRT"
     gene            complement(56409..57467)
                     /gene="prfA"
                     /locus_tag="PKV49_00730"
     CDS             complement(56409..57467)
                     /gene="prfA"
                     /locus_tag="PKV49_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPL22177.1"
                     /translation="MHQELKTKLAELNSQLNDPAIIADNQKFRRLSKEQAELASLVSL
                     VTRLESIEQHLAEAGQLLTESTDSEIRQMAELEQAQLTAEQAALSQQLDEALHPADPR
                     DKKDVIMEIRAGAGGDEAALFVAEIFRMYSRFAENHGFKLEILNSNPIGIGGYKEITC
                     AISGQQVFGWLKYESGTHRVQRVPETEKQGRVHTSTITVAVLPEAEEVDIEIKPSDLR
                     IDTYCASGPGGQCVNTTYSAIRIIHEPTGLVVTCQDQKSQHQNKEKALQILRSRLLQK
                     KEDEQREAADNQRRQQIGSGDRSEKIRTYNFPQDRVTDHRLQESWHSIDRVMAGEIDE
                     IIQHLQQYAKQLAQEPDR"
     gene            complement(57536..57841)
                     /gene="rpmE"
                     /locus_tag="PKV49_00735"
     CDS             complement(57536..57841)
                     /gene="rpmE"
                     /locus_tag="PKV49_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPL22178.1"
                     /translation="MKKDLHPTYYSEAKVTCACGNIFTTGSTMAEIKTEICSACHPFY
                     TGKQKLIDTARRVEKFAAKLESKTEIRSKKTKREARNKAREAKAQAAKEALEKELAA"
     gene            58006..58446
                     /locus_tag="PKV49_00740"
     CDS             58006..58446
                     /locus_tag="PKV49_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22179.1"
                     /translation="MPNFEAFPDSNHVEENEQNKQEQEQAQEQEQTQEQEQEQAQEKM
                     LRFMLNRRYDESGYEHPFDQIQNAMRDYPALSQEDIVRIYLDEQEYETVANFIDKFPE
                     VDHRAVAAKLVEAGKGSHVLRNLDKFNGVSREDVERDDWQPEAE"
     gene            58617..59345
                     /gene="rpsB"
                     /locus_tag="PKV49_00745"
     CDS             58617..59345
                     /gene="rpsB"
                     /locus_tag="PKV49_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPL22180.1"
                     /translation="MSQVPTLADLLAAGLHFGHKTSKWNPKMKPYIYGSKQGLHLIDL
                     NKTQEQMAKAWTFITKEVAAGKNILLVGTKDQVKEPLRRVAETTNMPYVTEHWLGGTL
                     TNFFIIKNSIRKYKDIIDKKKTGQLAKYTKKEQLEFDREAARLEVGVGGLVNLIKVPE
                     IIFIWDIKKEKTALAEALKRRIPIIAICDSNTNPTGIDYPIPANDDATKGIELVLNSL
                     EQAIMAGKQQREAAKESHQPVIKE"
     gene            59419..60228
                     /gene="tsf"
                     /locus_tag="PKV49_00750"
     CDS             59419..60228
                     /gene="tsf"
                     /locus_tag="PKV49_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPL22181.1"
                     /translation="MEITMEIIKNLREQSGAGIVDCKTALTEAGGDLTVAMEILRKKG
                     IAKAGKRAERETSQGIIKAWVSPDQRRGHLIELRAETDFVARNEQFQGLADNILQVLV
                     DHQPSDLAGLLNLSLGSQTVREAVDNLSGVIGEKIVLSNCATLTSQGTVAAYLHAGGS
                     IGVLVAISQAESGDLAKDIAMQIAASNPRYLTPDQVPMTEIEKEKEIYREQLSQEGKP
                     AEIIEKILAGKINRYFTEVCLTKQEFIKDEQQTIEQILGGAVIEKYYRFQL"
     gene            60250..61107
                     /gene="ricT"
                     /locus_tag="PKV49_00755"
     CDS             60250..61107
                     /gene="ricT"
                     /locus_tag="PKV49_00755"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPL22182.1"
                     /translation="MKLALVQIYPWDRPSLCEPSELTIEIGQRLLVETDSGQELGRVL
                     GFKYLSNDQLAAWSGHTKKIVRLANSDDCQTVDQFDKTAALSQCQQFIDQCQLEMKLI
                     DAAVSADNRRLTFAFTAEGRIDFRELVRLLTRNFNKQIRLQQIGTRDEAKLFGGQGVC
                     GRGLCCRGFLTELSSVTSDMAEIQGINHRGSERISGICGRLMCCLAYEAEGYQAMAQK
                     LPNIGDQWKVNGQLGQVINRHILKGTIIVEFHDSQAGGQTVVEITPGQTTNKKDASVQ
                     RRWIGRHHK"
     gene            61112..61288
                     /locus_tag="PKV49_00760"
     CDS             61112..61288
                     /locus_tag="PKV49_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014733141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPL22183.1"
                     /translation="MLTIDQVNCIGCGLCASLCPDNFKINDDFKAEVVDGDQLKSCVQ
                     EAIDNCPVQAIKKA"
     gene            61346..62671
                     /locus_tag="PKV49_00765"
     CDS             61346..62671
                     /locus_tag="PKV49_00765"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPL22184.1"
                     /translation="MLTLEQVKQLKIPSCSGSYQFCVQDGSIVYVGKAINLQKRIYSY
                     WQRYQDLTPAKQQMIAEVVTIKWLETDSEIEALLLEANLIKKYQPKYNIVWRDDKRYQ
                     YIAISAGNFPRVYLTRKLEESGQFFGPFTSNQAARETLRIIRKIWPYRSCRNLPTKTC
                     LYYRIGQCPGPCQQLISAVDYQKIIQQIVLFLQGKKQSVIKKIQVELSGYQKQLAKLN
                     QPTKKQNASEEAAKFEYKISSLNWQLRQLEQVLASNHILGLEEKYANDVVELAKLLGF
                     TKPPQRIEGYDLSNLYNKQAVGSLVVFIDGEACPSEYKRFRLSGAETVSSLGDIALLR
                     EMLTRRLAHHDWPQPDLIVIDGAKAQANIARRLLDRAGWNIPVIGLGKDKGLRSARAM
                     DKLFFPGQQQALQLSLNNPALHLLKRVRDEAHRFAITYHRQVRRKKFWE"
     gene            complement(62674..63276)
                     /gene="recR"
                     /locus_tag="PKV49_00770"
     CDS             complement(62674..63276)
                     /gene="recR"
                     /locus_tag="PKV49_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPL22185.1"
                     /translation="MAYPPLVQQLIRLFSQLPSVGPKTAERYIFYLLEQKPELIEPLA
                     DHLQQLNQALTTCSLCGAISLTDPCFICNDANRLNNLLCVVATNQDLEAIENTGDYQG
                     RYFVLGGYLDTINEIKPEDLRLAALADYVQAHPAIKEIILAFDPTIEGETTALYINKL
                     LEKWPGQISRLARGLSTGANLEYADPLTLANALKFRQEIK"
     gene            complement(63280..63558)
                     /locus_tag="PKV49_00775"
     CDS             complement(63280..63558)
                     /locus_tag="PKV49_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPL22186.1"
                     /translation="MFNKLKQFKDLRNQAKTMQNALAGEKVTAEKQGVTIEMNGNMEV
                     LSLVIKQDLPKEQLANICRDVFNDAVKKTQRVMAKKMQEMGGLSNLGL"
     gene            complement(63568..64950)
                     /gene="dnaB"
                     /locus_tag="PKV49_00780"
     CDS             complement(63568..64950)
                     /gene="dnaB"
                     /locus_tag="PKV49_00780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPL22187.1"
                     /translation="MASKNNTIKMPPQNQEAEISLLGCLLIDKAAIVKIADIVKPEDF
                     YNDINGLIFAAMLALYQNHEPIDLVNLTDKLKAKNQLDTVGGRSYLTSLLQGVATAAN
                     VVSYAELVKHKSTLRRLITAASEIGELGYREDDDLAQVLDQAEQKLFGVSHQYLKQSF
                     QPISGLLTDAFDRIDELHKQSGRLRGLPTGFVDLDRLLSGLQKSDLIILAARPSVGKT
                     SFALDLARQAAIKSRAKVGIFSLEMSKEQLVDRLLCAEANISLWKMRTGQLSDREDDD
                     DFGRISYAMGALAESSIYIDDSPNCNIMEIRTKCRRLQSEHGLDLIILDYLQLMEGRG
                     RINDNRVQEIAEITRSLKGLAREINVPVVALSQLSRAVEQTKPAIPKLAHLRESGSIE
                     QDADIVMFIYRKAADRNYDTENLNDFEKHVAEIHIAKHRNGPTGKVDLYFDENTVNFK
                     NLDKSGITAG"
     gene            65098..65847
                     /locus_tag="PKV49_00785"
     CDS             65098..65847
                     /locus_tag="PKV49_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPL22188.1"
                     /translation="MPHRFCPLLVVGLLLVAVPALANGLPDTDGDGVPDQDEINIYQT
                     SVDQADTDGDGYSDRQELLAGYSPHNPKNIKLIDNDQDQDGLSDFWELRFSTNLLMAD
                     TDGDNINDGDEIDQATDPRQVGAVKLPIRLDVSLKRQELTYWLAGVKFKQFPVSSGAP
                     RTPTPIGQYRVINKLPKAWSPYGLWMPYWLGLGRGRFGFHELPIWPNGYREGANHLGK
                     AVSHGCIRLGIGPAQYLYDRVDVGTVVNINR"
     gene            65869..66243
                     /locus_tag="PKV49_00790"
     CDS             65869..66243
                     /locus_tag="PKV49_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPL22189.1"
                     /translation="MLYSPQVIQHFQKPHNQGSLAHPSAVGQVGNPVCGDIMKIYLRI
                     KNGVITKVKFETLGCAAAIAVASALTDLVKGKTLDEAYQITKDQIIDDLGGLPAPKVH
                     CSMLGVDALHLAIDNYRQQLVK"
     gene            66253..66504
                     /locus_tag="PKV49_00795"
     CDS             66253..66504
                     /locus_tag="PKV49_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPL22190.1"
                     /translation="MDKIKNLAWWPELEKIIDREIRPSLQMDGGDIRLVDFDEAKGIL
                     SVELQGHCAHCPMMEYTLKEGIEAELTARLPKIKKVQAV"
     gene            66507..67736
                     /locus_tag="PKV49_00800"
     CDS             66507..67736
                     /locus_tag="PKV49_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPL22191.1"
                     /translation="MKVYLDYSATTPVDPLVYKAMEPYFSQVFGNPASLHHCGQAAQA
                     AVSQARQTIADFFGAQAKEIVFTSGATEADNLAVKGLIQGLLKQKPDLRPHIIVSSVE
                     HEAVRQPCQQLAKEGIEISWLPVDKKGLVDLVKLEKLIKTNTVLVSVIYVNSETGARQ
                     PIREIGQMIKKINHRRQEDWRKRSGLANKRPQTVYFHTDATQAINYFDCRANYLHCDL
                     LSFSGHKIYGPKGIGGLYVRSGTPLAAIQLGGHQELNRRSGTLNVPGIVGLAKALELV
                     NRHRAADNRKIAALRRRLVQGLVKNIPDIILNTDLSNASPSHAHFSVLLAEGEALLLA
                     LDLAGIAVSTGSACAAGSLEASATLLAMGISQEVAHYSLRFTLGRLTTVTEINYVIKK
                     LPAIVARLRRWAPDLNN"
     gene            67738..68151
                     /locus_tag="PKV49_00805"
     CDS             67738..68151
                     /locus_tag="PKV49_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22192.1"
                     /translation="MRLLLSKLLNYFYLFGQNWRITWSDIWHDSGHRWLTIIDIILLV
                     ISWILAFWLAAVGRGSLLILHYNINFGIDLIGSANSIYWLPFGATVAVVVNSLLLQIN
                     YPAAKQLRLTSLAGSLAVLILINLALASLLLINFC"
     gene            68159..69211
                     /gene="mraY"
                     /locus_tag="PKV49_00810"
     CDS             68159..69211
                     /gene="mraY"
                     /locus_tag="PKV49_00810"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPL22193.1"
                     /translation="MFDFYIIRTLLLAAGAFIFTIAWTPLLTNFLYRHKLGKKIRNSG
                     ATPIFSQLHAAKSGTPTMGGLLIWVTTLVMALGLFLVNKLWPGSWLGQLSFLSRSQTY
                     LPLGILVASALIGLFDDWLDVRGRGTKGGGGLSLRSRLLVYAIIALIGALWFYFKLDW
                     DIFHIPFLGNFSLGWWYIPVFIFVIIGTAFSVNETDGLDGLAGGTLLSSFMIYGAITF
                     LIGRYDLTALCAVISGALLAFLWFNINPARFYMGDTGSMSLGITLGVIAMLTNTALIL
                     PVIGLVFVVETLSVIIQVLSKKLRHGRKVFLSAPIHHHFEAKGWTEPKIVMRFWVIAA
                     VSGALGVCIFLLDHTI"
     gene            69217..>69867
                     /locus_tag="PKV49_00815"
     CDS             69217..>69867
                     /locus_tag="PKV49_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPL22194.1"
                     /translation="MKSVVIKKKTTIAEKRSTAQPIARVTRQPLDRFLLITVVLLCAI
                     GLFCLASSSSVVAYTRYGDTYFWLKKQVLSMLIGAAGFYLFSLIDYRKWQKLGGLMLI
                     IAVGFLLTLFTPLAHEQAGTTAWLNIFGFSLQPSEFVKLFLILYLSALMAKLGSSVNP
                     EEKQRLNWFFWLVLVIIGGLILAQPDLGTLLIMVATSIVIYLLGGGSWKLVGQLTAV"
BASE COUNT        19030 a        13095 c        16312 g        21430 t
ORIGIN      
        1 cgccattact ctggttggcg gagttaatgg ttttattccc caagctgagg ccggaaccca
       61 atgttttata agatttaatg ttttggatag taaaatttat agtcttgaaa ccgttgagac
      121 aatattgctt aaggctaatc tgattaacag gggcgatttg actggatcta ctcaacaaga
      181 gcgccgcatg ccatttgatt ctaaggtggg cgacagtcag gtagttaacg aaactcttga
      241 taatttacag attaatttat gcggcaaggt tgatggttgc gataccagga ctttggatat
      301 ggaagtaatt tgtgagttga gtacatcacc gactggcaac actattcgtg atgctcaatt
      361 ggcctatcgt agtcggataa ccgaaatgaa tgcttgccag cagggattct ttaagaagat
      421 tcgggccttg aacagtcaat atgtcaggga tgtccgggcg gctaatgttc aatattatca
      481 acaattggca ccgagaaccg ccgctttatt aaaatcttat cgtgcagccg atgacaagga
      541 caagcgcgcc attttagacg agtatatcac tcataatcag atattgcgta gggatttata
      601 tacgacacaa acaattattt ttgaaacatt tattaattcg cggactgctc tccaaaataa
      661 ttttaatttt tgcggctcat gattgacttt aagcacattt taagcgtcat gaatttagat
      721 aaaccattgg tggtttttga tttagagacg actgggttgg cgttagctta tgatcggatt
      781 attgaattgg cttttgttaa aatattgccc aatggtcggg ttattgaaaa agatattttt
      841 tttaatcctg agattgacat tccgagcgaa gttacagcta tccatggttt aaccaacgct
      901 gatttagtgg acaagccgct ttttcgtgaa caagctcagg agatatggga tgtttttaat
      961 gactgttatt atggtggttt taatattgcc aattttgact tgccaatgtt gcggcgagag
     1021 ttcttgcgcg ccggttatga ttttaatttc accaaaaaac aaattattga cagcaaaata
     1081 atttatcatg ccatggagcc gcgcgacctt gaagcggctt ataaatatta ttgtcaaaag
     1141 aagctagttg atgcccatag tgctctagcc gatgttcatg ctagcgtaga aattttagat
     1201 cagcagctaa ttaaatatga ctatcaaaca atccgtcaga ttagcgaggc tccagacgac
     1261 cgttatgttg atgaagaaaa gcgtttttat tggcgtggcg gtgaagccta tttgaatttt
     1321 tctaaacaca aagaccgctc tttagctgat gtagttaagg ttgatccggg atttttgcag
     1381 tggattattg gcgctgattt ttctccggaa actaaacaaa tagtggagcg agcattgcaa
     1441 ggcgagttcc cgaaaaagag tggcgctgtg gcggttacta aagacaattt gccgttaact
     1501 aagaaatcag ctagcggttt tactgataag ccaaaattat tttaagccga tgtctgttaa
     1561 tagtaatagc cgtgaccatt tattggcggc tgatttaagt gatgccagcg atcaagattt
     1621 agatttgagc ttgcgcccaa aaaaattgtc ggaatatgtc ggccagccag ctgtccggca
     1681 aagcttagag atatttttgc aagccgctca gtcgcgcggt gagaatattg aacatgtttt
     1741 actatatggc gctcctggat tgggcaagac cacactggcc tatgttattg ccaatgaatt
     1801 aggggctaat ttacgagtaa ctgccgggcc ggccattgaa aagtctggtg atttagcggc
     1861 cattttgact aatctgcaag atggcgactt attgtttatt gatgaaattc atcgtctgcc
     1921 gcatagcgtt gaagaaatac tatacccagc tatggaggat cgagttttgg atattgtttt
     1981 gggcaaagga ccgtcagcgc gaactcttag gttggattta ccgcatttta gtttaattgg
     2041 cgccaccact aaggttagct cattgtcagc gccgcttcgg gatcgtttcg gcatagtcta
     2101 tcgattagat ttttataact cgaatgattt acaaaaaatt attagtcgca atgctaaaat
     2161 tttacaattg gaaatagaac cgcaggcgca aagcttgatt gctgatcggg ctcgccgaac
     2221 gccgcggatt gctaatcgtc tactaaaaag agtccgtgat tattgccagg tgcgcggcac
     2281 tggagctatt actttagctt tatgtcggca ggcttttgat ttattagcgg ttgacgattt
     2341 gggattagat gaggttgacc gttatttgct aactactatt attgagaaat ttggcggcgg
     2401 tccggttggt ctcaatactt tagctgccgc aaccggtgaa gagttaatga cgattgaaga
     2461 tgtttacgag ccctatcttc tgcaattagg gtttttagaa agaacgccac gcggtcgcaa
     2521 agccacggtc ctggcttata atcatttagg cattatgccg ccagccggtt tattctgatt
     2581 atggatttag ccaattttga ttattatctg ccgccggaat taattgcgca gacgccggct
     2641 gagccgcgtg atcaggctag gttaatggtt gttgatcgac agaccgggtt caggcagggc
     2701 gccattttta gtcaattgcc cgacttttta caaattggcg atgttttatt aattaataat
     2761 tccaaagttt taccagccag gctattggga cataaagtcg gtagcggtgg tcggattgaa
     2821 atattgctag aacaaaaatt atctcagccc aataatggag aatattggcg ttgcttagtt
     2881 ggcgggacgc gtcccgggat cggtttggtt gttgatttgg gggataattt gcaagctgag
     2941 atcatggccg ctgagactga cggagtcttt gtggtgaaat tcaatcaaac ggggactgat
     3001 ttctggtcag ctatttatcg tttggggcaa atgccattac cgccttatat taagactgat
     3061 cagattggcc tgcagcgagc cgttaatgat tatcagacta tctatgcgaa agccgataaa
     3121 atcgggtcag tagctgcgcc aactgcgggg ctacacttta ctgaaacttt gttgcggcag
     3181 cttaagaatc aaggagtaga tattattgaa gcaactttgc atgttggtct aggaacattc
     3241 gctccggtga gacaacaaaa cattaaagat caacggcttc atgatgaaat ggtagacatc
     3301 agggctgaaa ccatcaataa acttattaat tatcatcgcc aaggtcgacg cataattgct
     3361 gttggtacca cagcggtgcg tatcttagaa ggcgtggttg ccaaatttat tgacaagcca
     3421 gccgataagg actggttagc accgattaat ctttttattt atcctggttt taaatttaaa
     3481 ttattgggtg gactggtgac taattttcat ttgccgaaat ctagtttgat tatgctggta
     3541 tcggcttggg ccggcaccaa gctgattaaa caagcctatc aagaggcggt cgagcagcat
     3601 tatcgatttt ttagttatgg cgacgctatg ctcttggttt gattaatgat taagttgacg
     3661 aaatcactag tattcattat actaaacaac gctaaggttt attaatttta aattattgat
     3721 atggttcaaa tgaagaagcg gaccaagagt gaaactcgtg gccgtcggtc acatcacgct
     3781 cttaaggcta aaactttggt gaaatgtcca cagtgcggcc agatggcttt accgcacact
     3841 gcttgcgctt tttgcggcac ttataaaggg cgtcaagtga ttaaagtgaa gtcagctgct
     3901 ggtaaaaccg ctaagccagc caaggcggcc aaaccagcca ccaaagacaa agcaactaag
     3961 gctaaaaaag ccgaataata ttatggctaa tcgtcatctg gccagaacta ttgccatgca
     4021 gactttgttc tgctgggatt ttaatggcca aaaagataaa ttagaagatt tggttaaaga
     4081 aagttttcag caatttgctc ccgagtttga cgatcatggt tttgtcaatg atttagtcaa
     4141 aggggtggtg gctaatttac cagcgattga taattatatc gtcaaatatg cgactgaatg
     4201 gccgcttgat caaattacac cggtagatcg caatattttg cgtatcggca cttatgagct
     4261 agtttttgac cagaatattc cagccaaagt ggcgattaat gaggcgattg aggtggctaa
     4321 gagttttggc ggcgacgctt ccgggcggtt tgtcaatggc gtactcggag cggtttttaa
     4381 agagattccg cctaaagata ttgataagca gcccaagggc cagccagaaa cagctgaggc
     4441 gatgccagcc gaatagctat tatgaccaac caacagcagt tagctcgggc gctgggagtt
     4501 gagttcaaag acgacaaatt attgactcaa gccctagttc atcgatctta cttaaatgag
     4561 cacaataatt ttccagttag ccataatgaa agactggaat ttttaggcga tgccgtttta
     4621 gaattgatag tgacggagtt tttgtttttg aattatccgg ataaagccga gggcgaatta
     4681 actgattggc gagcggcatt agttaatgcc aaaacattgt cgcaggttgc taaagaaatt
     4741 catctggatg actatttatt tttatcacgc ggtgaagcca gagaaacagc ttctaaaggg
     4801 cgacagtata ttttagccga tgccatggag gccttgattg gagctattta tttagatcaa
     4861 ggctggaatg tcgcccagcg ggtgattaat caattagttt taactaaact cggcaatatt
     4921 attaagaata agctttattt ggatccgaag tcttattttc aagaacgggc tcaggaaacc
     4981 tatggtttta cgccccacta tgaagttttg tcgtccagcg gtcctgacca tgaaaagact
     5041 tttgaagtcg gattatattt agacgatcgt ttgattagcc gtggccatgg catgtctaag
     5101 caagaggctc aggtggacgc tgctgagaat ggtttgaagc aggagggctg gcgctagaat
     5161 tttgattagt cttaggccaa agcagactaa ttagaccaat gccaattaat acatcggcca
     5221 aattaaaaac tgtccaccag ctagttagat aatcaatcac tccaccataa actaggcggt
     5281 cataaagatt actataggcg ccagctaata gccatagcca accatccaat cgtgacggtt
     5341 gtttttttat tgcttgtcgg ctgtaaatag ttaatccaat aaccaaaaat attaataacc
     5401 aattaaataa tggcccattg cccggtaaag aaaaggagag ccggcgattg agattatagg
     5461 ttagagaaaa agaaccgcta agctggacat gattttgtga ccaatagatt aatgagatta
     5521 ttttgagagc gcgatcaatt attaataaca aaacagccct aaatagggct gctcgaactg
     5581 gatggcgacg aatagctagt ttttttgcag ctggcttttg catttgacgc acgagctaga
     5641 gaatggccga gctagcaacc ttttttcatc aatttcttta tggcaatatt tacaaatacc
     5701 ataagtattt tgatcgattt ttttcaaagc cgatttaaca tcacgcaaag tcttttctaa
     5761 aatattctcg gtcgctatat tggtagaata ttggccaacc tcttgagcgt tttcatcatt
     5821 ttcttcacca taatccggga aggtgctggt aaagtcattg gcattgccag aatctttggt
     5881 agcgaaaacc ttcagggctt cttcagtttc ctgtttgatg cgcaagagtt cagctttaat
     5941 ttcttgaata gtttctggtt tcataataaa aataagggga agataattct tcttgctgat
     6001 ttaagtatag gcaataaaat agattttggc aaggcggccg agattaaatt cgtggttcga
     6061 gccaactgat aagtcggcct ggcggttgat gttggaagtt aataatttta gcttgttgag
     6121 gtagccaatc agccactatt tccggccatt caataataat caagcagttc ggttgtttaa
     6181 cccaatcagt aatccctaaa ttttgtaaat cttgaccgcc agttaaacga taggcatcaa
     6241 tatgtagcag ttttttaata gtcggatgct ggcaatcgta ttcttgccaa atagtaaaag
     6301 tcggactgac taatggttga gcgattttta agtatttggc tactgattgc acaaaggatg
     6361 ttttgccggt acccaattct ccagacaggg caataatctg gccaccttgc cagtttttag
     6421 ccagttggcg agccaagctg gccgtttggc gaacggtggg cgcatagtat tctaccagca
     6481 attggttatt cttgggtttc aataacatag tagccgttgt tctgataggt ggttgtaaaa
     6541 tatggcgaaa tgaataattg gcgatcaaat ttgagttctg cttctttaat gccgctgata
     6601 aattcgtcta aatgaaaata gagataatag tatttttggt cgctgtagaa aatcaagcgt
     6661 tttaattttc caaaagggca ttgtttaatt ttttgctcta actcagccgc tgttttagca
     6721 gccgctaaac tgtgaacata gttatagcgc tcagaaaata ttgaagccgg atggccgtta
     6781 tgttgattaa aatagagcaa attattaatc ggggtaaaag ctaaaagttg tggagtggtt
     6841 tctagggtca actcgtgggg cgatattgga ttagccgtta aatagttagc caattgaata
     6901 atagctgggt cagcttgacg agattcaagg cgtcgatttt taatagccgg atgatcaata
     6961 aagaaaccga agaacgattg gctgattaaa taaagcagac taagtagggc aagtgttatt
     7021 aaatagtttg ctttttggtt gacctgataa taattccata gtcggctcag gccgtaggcc
     7081 gcgcctaagg ctaagatggt tggcgcccag acgtaaaagc cgcggaattc ttggaaaggt
     7141 ttgtgccaga ttagtagcaa taacaggccg ccaacccacc agataaagga agtggacaat
     7201 aaataaagta attgcttagc gattggttga cgacgataat aaattaaagt agctagaccg
     7261 aatagtaaaa ttagattagt ccagctagtt aattggaaca ttggtagcca cagatctaag
     7321 ccgctaggtg taaaaaagac agtctgccaa ttttctaggc cattttttaa gtagctgcct
     7381 attaacggtc cggtgaatgg taaagtggca atcaagccga ctacggcggt ttgacccaat
     7441 cgccagaaat aacgaagctt gtgctggttg ttttctagta gccccattaa aataaaggct
     7501 atcgcggcaa agattagcca tagataataa gtcatgaaaa ttaagccagc ggccaggccg
     7561 tagacaatag gtagccaaat tttccaacgt tgaccaatcg cctgataatg aagactaata
     7621 gtccatagta taaccattac tgctgaaaca atttcatagg gcttgcctag cattaaatct
     7681 ttatccaaga cggacataat caataatggg gacaagacag ctaagagagt gttgagacgg
     7741 ttatcatcta gaatattgct aaaaagacga cgttgcaata aatataccac tgccgggaaa
     7801 attaataaga aggttgtggc ggctgcctta atcataatga tgccgttcca gtggaaaaaa
     7861 cggccaatta ggccaaaaag ccaaaagcta gccggcgggt agaatggtgg tagatggtgg
     7921 tagctaaaat cagcgccgag gccgattttg gcaaaggtgt tgaacatagc ggcgatagtc
     7981 aattcgtcgc cctgtaaacc ccagagaggt aattggaggt tattagacca gagaaagtag
     8041 gtgctagcgg aagttagtcc aaaagcgatt aacaagtaga gaggattttt tttcttaata
     8101 attaagtagg ttgccaagat ggcaaaaagc accaaaatta atttgatgcc actgtagatt
     8161 tgaatatcaa gaaaagtcat aaggttatga gtgggcgcac aaggagttga acctaggacc
     8221 tcttcggtgt aaacgaagcg ctctaaccaa gctgagctat gcgccctaat ttactctctg
     8281 tggtgagaaa attcgcgagc ccaaaaagac ctcactgcag agaataaaat ttaatcaaga
     8341 aatcagaaga ttactccatt atacgttttt tgtattcttc ttctgcgtct tcggcatcaa
     8401 gccagtgacc ttcagcgatg ctatggattt taccgccctc cattctcggt tgtttaattt
     8461 taccagccgc aaataagcgt cgcgcaatag cttctccttc ctctggatta tcgtaccaat
     8521 aagatttttt gtacaaatca atgagttcat caaatttttc cggtgcaaaa ctagtgccag
     8581 cgataatttc tttaacctct tcttctttaa ctttaccctc taaaatgttt ttaatacaaa
     8641 gacttaagct taaaccgact ttattttcgg ttgtggcttc ttgagattcc cgttgagaat
     8701 cttgtggcct aaattgctcc aacataagtt tagttttaaa agtaatttta taaattttat
     8761 agaaagcgaa ttggctttct atagtggtgc cgaggacagg acttgaacct gcatgaagtt
     8821 tccttcacaa gcacctcaag cttgcgtgtc taccagtttc accacctcgg caagccgatt
     8881 aagcggcttt tttggcgata gctgttttct tttcgctaat atctttaagc cgttttttgt
     8941 aacctttttt caaaaaggtc agcttagccc gacggacaat agcttgcttt ttaatttcta
     9001 ccttattaat cgttggtaaa ttaattggaa agattttttc tacagcaaca ccattggaaa
     9061 ccttacggac ggtgacagtg ccgccagctt cacggccgtg tttgcaggcg ataactaacc
     9121 catcaaaata ttggatgcgc tctttttctt cgcccttggg attgagttct ttgatgcgtt
     9181 gataaatacg gacagtcatg cccggtttta gatcgcccag agtagtttta gtcggctgtt
     9241 cttcggttgc cgcaacgact agtttagttt cggtcataaa tttatagtaa ttttcggctg
     9301 ataaaaatag cgcttatttg gttaagcttt attagcttag gtaattttag gggtattgtc
     9361 aagattttat tgactttttg gcaggatttt tttaagtatt gtccagtaaa actttgggga
     9421 ttagcggcaa tttctagtgg agtgccttca gcaataattt gaccgccctt ttgaccgccg
     9481 agtggaccta aatcaattaa ccaatcggcg gttttaataa catctaggtt atgttcaata
     9541 acaatgacgg tattgccttt gtcaactaat tgttggagaa cttctagcaa tttatggata
     9601 tcagcaaaat ggaggccggt ggttggttca tccaggatgt agagagtttt acccgtgctc
     9661 ttgcgagcca gttcggtagc cagtttaacg cgttgcgctt cgccaccgga cagagtggtg
     9721 gccggttggc ccagtttaat ataacccaaa ccgacttcat tgagagcggc cagtttatct
     9781 ttaatggctg gctgattagt aaagaattcc agagcttcct caatggtcat ttccagaatt
     9841 tcgtaaatat ttttaccttt atagtgaatt tccagggctt cttgattgta gcgctggcca
     9901 tggcagactt cacaggtaac ataaacatca ggtaaaaatt gcatttcaat tttgactagc
     9961 ccgtcgccat gacaagcttc gcagcggccg ccagtaacat taaacgagaa gcggccagat
    10021 ttgtaaccgc gtaatttagc ttcgggcagg ctggcgaaca aatcgcgaat cggggtaaac
    10081 gctccagtgt aagtggcggg attggagcgt ggtgtgcggc caatgggcga ttgatcagtc
    10141 tcaataatct tattgatata ttgtaatccg tcaatacgct cgtgttgacc aggatcttcc
    10201 agcgcttggt aaaatttttg tttaagggca cgagctaaaa tgtcggtcac caaagttgat
    10261 ttaccagaac cagaaacgcc agtgatagcg ataaatttac caagcggaaa attaacagtt
    10321 aggttttgta gattattttg tcgggctcca ataatagtta agtaatggcc gttaccagcc
    10381 cgatattttt tagggattgg aatagatttt ttaccggata gatattggcc ggttagcgat
    10441 ttcggattag ccatgatttc tttagccgtt ccttgagcaa tgatttgacc gccgttttta
    10501 ccggcgcctg gcccgacatc aataatatga tcagccgcca gcattgttgc tttatcgtgc
    10561 tcaaccacta aaacactgtt gcctagatcg cggagatttt ttagagtgtc agttaattta
    10621 tcattatctt tggggtgtag gccgatagat ggttcatcta aaacatatag tacgccagtt
    10681 aaagccgagc caatctgggt tgctaatcgg atgcgttgcg cttcgccacc ggacagagtg
    10741 gtggccggac gattaagaga taagtaatct aaaccgacat tgagcaaaaa ttgtagacga
    10801 ctaataatct ctttaaaaat cagccgacca atttgggttt gtttagcagt taattttttg
    10861 ttagcgatta attgtttaaa gaaagtgtaa ttttgggcaa tagtctgctc ggttaattca
    10921 tagattgatt ggccagccag tttaacggct aaaaattctg gtttgagccg ctgtccgtga
    10981 cagactgaac aaggaatggt ccgcatatat ttttcaattt cggtgcggac aaaatcgctc
    11041 tcagtctgct gataacgctg atagaggttg ggaataacgc cttcaaattt aacgtttaat
    11101 tgattcatcc cagcggtgct tggtagagta tagactaaat caccggtgcc gttcattagg
    11161 atatctaact gtttggcagt cagcttgttc aatggtgtat taagatcaaa gccgtattca
    11221 ttagccacgg cgccgataac tcgcgtcatc cacagttgtc tggaatagcc gtaatgtgac
    11281 caagggcgaa cagctccttg ggcgatagtt aaattattat taaaaactag ttcaggaata
    11341 atctccagtt tagtacctag tccggtacag tgtgggcagg cgccatgcgg tgaattaaaa
    11401 gaaaaattgc gcggctcaat ttccggcagg ttaatattgc aacgcggaca agcgaaatgt
    11461 tgtgaataaa ttttttcttc agcctcggtc tggtttaaat caagggtagc aactagtccg
    11521 ttagccaaat ctaaagcttt atcaactgct tccgttaaac ggctataaga actcttatca
    11581 ttattaatag tcagtcgatc aatgacgata gataggttat gttttttttg tttatcaacg
    11641 gttaaatttt gggcttcttc caaatcatat aaattattat cccagcgaac gcgactaaaa
    11701 ccagctcggg caatagctag taaaacattt ttgtgctcac cttttttatc acgaatcagt
    11761 ggcgataaaa tagcaatctt ttgaccggta gcttgtttta ttatttgatc gactatttcg
    11821 tcaacggtat tggctttaat agggtcgcca caaaccgggc aatgcggttg accaatccga
    11881 gcgtaaagta atcgcaaata atcataaatt tccgtaatgg tgccaacagt ggagcgcgga
    11941 ttattcgagg ccgatttctg atcaatagag atagccggag ataagccctc aattaaatca
    12001 acatccggtt tatccattaa gcctaaaaat tggcgggcgt aagcggagag cgattctaca
    12061 tagcggcgtt ggccttcagc atagatggta tcaaaagcca gcgatgactt gcccgatcca
    12121 gatagaccag taataaccac cagttgaccg cgcggaatat ctagatcaat attttgcaaa
    12181 ttattaacgc gggcgccgcg aattttcagc caatcttgag agacggtttt agtggtcata
    12241 tcaataaaat aagttagctc aattaaattg gtattatagc gggaaatagg caattagtca
    12301 aagatggtta attgttatta gaattattga gaaaagaaat aaataatata gtgcaaatta
    12361 aaacaccata taagactatg gtgtttataa tctggtcggg gtgacccgat tcgaacgggc
    12421 gacctcagcg tcccgaacgc tgcgcgctag ccaactacgc cacaccccga aatggtattt
    12481 agtataagtt aagcttggta taaataaaat ctttggccaa ttaactcacc cgtatgctaa
    12541 ttatatatca cttaataaaa taaaaacaat agtgaaaggc tagtctagag aaaaagatta
    12601 cggttattag tataattttt agagaatcat tgcttgtttg ggagaatatt ataacgcgat
    12661 tggctgctta aaaacttatt agcaccatat taaagcatga tttttatgaa cgaaattgag
    12721 attctctggt cggattattg gcttcgtggt tacggcagct ataaaaaact atggagtatc
    12781 aactataata taatttttgc cgtcatgttt ttcaatgtct aaattagcaa aattaccttt
    12841 cgcgcttcgc cattcgttgg taaaacccca accattccat ttccaaacag caagatagtt
    12901 gccaatacat tgtggctttt gcccataatc tccttcaagg acaactaaat cgtttttccc
    12961 atcattatca acatcagcaa ttttgaattc acaattcggc tcaggaagat ttgaagaacc
    13021 ccaaagctgt ttaattgaac cgtcgctaaa acctaacaca aaaaaatgat tttttacgct
    13081 gacatcattt tcttcaatcc aaaatggttt tgatgcgcca aaactaccgg atttccaaag
    13141 agataaattt atctctgtaa tcccatcatt attggagtca gccaaaacaa aattatctat
    13201 ccaccagtcg ctgggtgatt gccaaattat tttttcgttt tctgtaattg ttaattggcc
    13261 attggctaaa ttataatttt cagaaaccga attattatct aaatctgccc gttcttgctt
    13321 ggtgataatt ttattattgt taaacgcgtc gttatagatg attttgcgca ctgtcttttc
    13381 aaaattatgc tggttccaca tataaacatc ttggttggct aatggaaagt ctaagcgttg
    13441 taaaatttta gcggctttgt ctttatccgc tgcttcgggt tgagccagat ttttcattac
    13501 caccggcaag agagaaattt tactaaggcc atttttggta aagtaaattt taatagctag
    13561 cccttctttg gtttccgctg attgcggttg atcaaaaata aaattaccta aactataaaa
    13621 aataaatttg cctttatatt tttctaatgt ttggactacg tgggggtggt ggccaataac
    13681 taaatcagcg ccggcgtcaa tggctgcatg agcaaaatta atttgtgaat tgtttggttt
    13741 ttcaacatat tcaacgccag agtgcataga aacaataacc aagtcagcat tttgtttggc
    13801 tgcttgcacg gcttcggaca gtttatcaat gcgcataaag gctgttccgg cgcgatttgc
    13861 tgacgcttca taatggttgg gcactacatc agtgtcgttg taagctaaaa aagccaattt
    13921 tattccctgt tgatcaagat aaatcggctg gtaggcttct tgatcgttta gtccggcgcc
    13981 gacatattta attccaacgg cattcaggta attaaaagta ttgtttaacc ctttttctcc
    14041 aaaatttggc gtgtgattat tagctaatga tacgatagat aatcctgctt gttttaacgc
    14101 ttgctcagtg ccgggatttg atctaaaaat cattgcaaag tcggggattt ccgctcctgg
    14161 tgtgattggc gtttccaaat taccaaaaac taaatcagcg ttttttaaat aatcgcgaat
    14221 tttaagaaaa gggtaattga tattgttttg tcttttcacc attctttcta cggctcgtga
    14281 atatgaaata tcgcctaccg caatcataga aacttcctgg cttgcttgag gaagttgctt
    14341 tttttctgat aaaaaatcta aaaattgttt ttcgttgcgg gcctgattat tgggcgttag
    14401 attatttggt cttgcttgat ttttatcaca agactgataa taattaacga ccaacccaac
    14461 ggtcagagcc gccagaagaa caataattgg ccagatgatt ttgggggaca tttaatttac
    14521 tcggcgacaa aagcgttagt ccaagatggt agagctggag ctgtctccga atttaataat
    14581 tcgcgccatt tttcatcagt tagcctgtcc gccatcggcc aagtaaattc ataataagaa
    14641 tatactccgc ctttagcaat tcttaattgg ccatcaatcg gtaccaccac ataaatttca
    14701 aagattttac cgataccttc ttccaaaatt tgaccgttcg ggtcggtggc cacatcggcg
    14761 ataatggcgg cgggattttg gtctaagaaa tgtttttgtc ctaactcttt atactgaggc
    14821 tcgtctttat ttatttcaag ccagaagtgc tctaattgtc cgccaaatga acggataagt
    14881 tcgtattcat catcggttaa tttttcatta ttcaattctt tttccgagat tgtcttgagc
    14941 gacattgcca actgctccat tttatttaaa ttgtctttca tgctcgtcgt tagcaatcct
    15001 cgcgtttcta gtccttcttt ggtcattcgt aaaagcgagg ccagacgagc ataaacataa
    15061 ggattgggtt caacatagcc acgatcgtct ttttcttcag aaccatcgcc gccgagctca
    15121 gcataaacct gtttagcata aagaatagta tcgtgtttta gctgcgacca actacctaaa
    15181 aatgtgttaa gttcttttct gacccaggcg ttattttgca taaatattgg gtagccactt
    15241 gtttttttat caagaagcgg acggagctgg tgtagccaac cccaatataa attttgcgtc
    15301 caattttcag ttggtaatgc agcaagatat gtctgcatgg tggacatatt tttcggataa
    15361 caggcatatt gcgtttcgcc ttgtgttttt aaaatattca aggcctcgtc agagcccata
    15421 gtcgccggaa tatccaatcc ttttggcaac attctgccgt ttctaaacgg agcattggcg
    15481 caagattctg ctttcggtcc gacctctcta actactaatc tttgaaaaat agccgcgtca
    15541 atggtaaaac gttgacccat aaatctaaat cccttgacct cttcttctct atccggctga
    15601 atggtcgagt ttaaaatcgg tattgaattt atttgtggtg gctccaagct ttctgttgcc
    15661 ttgattaaag aggcgaattt attgttatcg cccggtattg attgaatggt catcttggcg
    15721 ccatatactt gttcaactaa atctttaaat tggtaataag tgatatcgtc acttttgcca
    15781 acaaagaaat tgactggttc ataaatttta ttccagctgt tttgattatt ttcttgatta
    15841 agcgctagag taatcagtag tgccgatttt acttcgtcat tatttttcat tctaaaggta
    15901 aggcgaccat accacatcat tgatttaaaa taggccttca attgttcggt tttgtcataa
    15961 tgacctcggg gaatgtattg cgaataatct tctttgaatg cttctaatga ttgctggccc
    16021 tgcggcgtgt caataatcgt gttttgttcg ctgccaatat tcataacagg cgatttttct
    16081 atgtcttggt gagcggcaat cagcgctaat tcttgattaa cttcgctagc gacaattgat
    16141 ggaatattta ctgaggaatc taatagttta ctgccgaccg caaaaaagcc gacatttctt
    16201 ttagcggcat tttcccattc tgttcctttt aagctattat attgatttaa tgattcagcc
    16261 agcatattag cattaagctg ttttaattca gccgctaatt tttgttcctc taattgctta
    16321 agtaagaaat caaacatcaa atgataattg tgaagcatcg agtcggtggt aataaaactc
    16381 ggtatatagt catagcgatt tgtttcgtaa agcgagaaaa attcattatg ataagatggc
    16441 ctcaccacaa aagcgttttt aataagcaaa tttttggctg catcagaaaa aataaaatcg
    16501 ttggcattag taatattgct cagattgggg tcaacagaat aagccggaac tttcgggttg
    16561 atattgaccg gtatgtcctc gtaggtggcg aatttggaag cgaatgccac ggtgggttgg
    16621 taaatagttt caaacacttc ctttatgatg ttttgctctg attttggcga taccaaataa
    16681 ataaccagag taacaataac aactatgtct aaaattccca agataatcca aaaagttttt
    16741 ctaggttttg gtttggatgt agttggtggt attggagaac catcaggact atcaggcatt
    16801 ggttgattaa aattgtgttt ggccatattc ttggaattaa ttttagataa atttaattat
    16861 agtatattgg ctagctttga gagtttatgg tcgttctgat agtttggggc gacggtcgtg
    16921 ctcgcggcga ataatttaat gggttcccaa gcaggggttg aaaggggagt tttgtccatt
    16981 tttaaaacag tgatggtgga cttagcgaga atcgaactcg cgtctctgca atgcgaatgc
    17041 agtgtcgtac cactagacca taagccctga ctattattag atttttagcg gacacttact
    17101 taatcgttat ctaattcaat accaataaaa caatttttcg tcaagccgat attaggttaa
    17161 actcggtgct gttgtctaag ttgctgatct tccagagagc gttcttcggc aataactgtc
    17221 ttggctcgtt gtcgtaattc ggccaggtta agattaatca gcggttgagc cagttttaac
    17281 aagacttcgc gttcattaag ttgaggatta ttaataacat cagctaagat aacatctagt
    17341 atagcgccga tttgcggccc aggttctagg ccgaattttt gcattaagtc ggtgccattg
    17401 atttttagca ttttaactga aacgggatca tgacgaactt tatcaaacat atactccagg
    17461 tggcgcaatt tgtagggctt ggctttcgct gtgccggaac caagccggtc agcaatgcgc
    17521 aaatcaatta aatcggccag attttgttcg ccggttttag caatgaggcg acggactgag
    17581 gcctcggtaa cttcgccgac attataataa aacatgtgat tacgcactaa attaacgacg
    17641 cggttaatct cttggttgga aaagcgcagc ctcgtcataa ttttttgagt caatttggcg
    17701 ctgatatatt catgattata aaaagtagtt tgtccattaa tagttttttt ggcgcgaggt
    17761 ttgccaacat catgtaataa ggcagctaag cggaccaaat agtcttggct ggggcaatgc
    17821 ttgagcgata gcaggttgtg atcaaaaaca gtataaatat gatgatgatt ttgcgccacc
    17881 ccatcgcctt cttccaattc aggcagaata tattgaagca atttagtttc atgcagtttt
    17941 ttaatacctt catacggctg tggtccggct agaattttaa ttaattcatc acggatacgt
    18001 tcgttagcaa tgaatttaat actgccggct aacttaatca tcgctcgttc ggttttaggt
    18061 tcaataataa aattaaagcg gttgctaaag cgcacagccc gtaataagcg cagagcatct
    18121 tctttaaaac gatcagccgg ttcgccgact gtgcggataa ttccttgttg cagatctttt
    18181 tgtccgccgt aataatcgat aatgtcgcca ttttgattca tagccattgc attgatagta
    18241 aaatcgcgac ggctcaaatc attttccaat ttatcttcaa aaattatctc actagggtgg
    18301 cgatgatcat cgtagtcaga ttcactgcga taagtagtga tttctaaaac ttcagtgcta
    18361 tcagttaaat aaatcggcca gataacagtg ccaaaactat ttttatattt gctgtccggc
    18421 aaggcggcca gaatatcagt tggtcgggca ttagtagtaa tatcccaatc tttaggctgg
    18481 tggcccatta ataaatcacg aacacagcca ccgacaataa aggcctcaaa accggcctgc
    18541 tgtaggatta ttaatgcttg ttgtacgaaa tcaggcagtg aagataagca ttgtttaccg
    18601 gcttgattgc ttttgctagt ttgtggaaga gagtcgggtt tagtcattgc tttggctaag
    18661 ttggagacta tttaataatt gattatagcg ggccaccgtt tgattgaatt gttcaattaa
    18721 ttgattgtaa tattgacgga attgttcata agcttgaaca gcttggttat agatggggac
    18781 cagttggtta tattggttaa tttggttgct attgcggtat tgttccagtt gctgctgctg
    18841 ttgttgtaat tcggtttgcc ggcggcttaa ttcaacttgt tgttggttaa tgatttgtcg
    18901 cgattggtcg ctggcgattt tttgttcact aatttggcta agagttgctt gcaggttgct
    18961 tagcccgcca taatttttgc cggcggttgc ttgatagata aggggttctg ctagattacc
    19021 acttggttgc caacttggca atgtaactga gctactgctg gcggcctggc cgttaatact
    19081 gctgggtagc aagccgatat gataaaattg ggtagtttct atatagcaat agccagtgga
    19141 ataattagca gcctgctgag gacaggcgat accaacagcc atatgattag cttcaggata
    19201 agacaataag gccacgccat agccaagctt acgaaaaatt aaccaggcca atagggcttt
    19261 atcagtacaa acgcctcgat ttaaatagag ggtttcataa gggtaattgg gtgtgatttg
    19321 attatttggt tgttggatta aatcgctgcg gttttggtcg taaggaatag attgaataaa
    19381 agcggccgct agctcagccg tttggtcggc agttaattgg ttggtcgttg cttgtttttg
    19441 taattgttca gtgatttggc gaataatatt atcttgtgaa gcattgttta aaaactgacc
    19501 ataatattga ttacgccaat tactaggtaa attgtcgcca gtgtaagtaa aaactttgga
    19561 ttgttgccgg tagtagtcat ataacttagt tgacaagtct agttggatag aataatctcg
    19621 gccagcatag cgccagcgcc aaagtttggt gtttggattg ttagttttag ctgctgtatt
    19681 gattatgcca gtaggaatag gatcaatatg ttcattagta gcgccgacgg ccgtttcttt
    19741 cataatgcga aaagcatcgg ccggccgacc tagatagtag agcttccgtt gactgggttc
    19801 aaagtaataa gccttgccct gatcttgcgg ttttaacaag atgtggccag acaatctttt
    19861 gaccttggct gaagctagcc gcttattctc taagagagaa aagtcagcat tgctgatacc
    19921 gatggctaaa ccgcgcataa tgcgaaaagc atcggccggc cgacccagat agtatttttt
    19981 ttcatcagtc gggttgatat accaagcctc gccatgccct tcaacggcta ataaaatttt
    20041 gccggcaaag gggctggcag cgctagcggg cgatattaca aaatagacgg acaagatagc
    20101 agacaaaata attccaccac tgattattat tttacgcata agtcaaaaga tgttatgtta
    20161 ataacataat tatagccgat ttttagctaa aggtgaattt atttttaata ataatttatg
    20221 gttaaccagc cgacagccca ggatttagtt gcttggcgtt ccatgattgc caatcggcag
    20281 aaatggcaat taatggtcaa agtgacggct aattgtcccc actgcggttg gcggggccaa
    20341 ttagcggatg gtcgttggaa aataggctta aatgagccgg cggtggataa tcgcgctaat
    20401 caggctttgc tagtctggct ttccaaagag ttaaattgtc cggttagccg tttggcgatt
    20461 agccatggcc ggaccagcgg ttttaaaact attgttgcca ttaattaaat tatgactaaa
    20521 aaacataaac caaaattatt gttgcatatt tgctgtggac cgtgcgccac agtagccgcc
    20581 aatagattaa tggaagatta tcaggtagtg ttgctttttt ataatcccaa tattgagccg
    20641 atagccgaat ggcaagaaag gctagcagca gctcagaaat tggcacagat ttacggatta
    20701 tctctgattg ttgatgatac tgattttgaa gattggcaaa caatggctaa accgttagct
    20761 attgaacccg agcgcggtcg tcgttgtcag ttatgctacg aatggcgatt acgccggacg
    20821 gctcagttag ccgatcaaga gagctgcgct tattttgcta ctagtcttac ggtcagcccc
    20881 tataaagatc gggaggcaat caataaaatt ggtttaatcg ccgccggggt gactagagct
    20941 aattatttac caaccgattt tcagcaagcc gacggctatc ggcagtcaat taagttatcc
    21001 aaacagtatg gattatatcg gcaaaaatat tgcggctgtc gttttagccg tcagtagtct
    21061 tttctgtctt gccgtgctat aataagccca aagttactat tatggattta aaaatttatt
    21121 ttgagcaggc aattgcccgt cgagcctctg atgtccactt agttggcggc agttgtccat
    21181 atttacgaat tgacggacaa ttaattaaaa ttagcgatga gataattgat gctgatcaac
    21241 tcgccaaggc agttaaggga tttctagatg aacggttgat tgaaaagtta gaagacaata
    21301 aagaactgga catatcgcag gcaatcgctg gtggccactt tagaattaat cttcattatc
    21361 aacgaggggt tattggttta accgctcgtt taatctctag tgatattcct aatccagaag
    21421 atattggttt agataagaat ttacaacgat tagctttttt gaatgatggt ttagttttag
    21481 tagttggcgc gtgcggttct ggcaagtcaa cgacccttgc cagcctgttg ggcattgtta
    21541 atcgtgagcg gcaggggaaa attgtgacta ttgaggaacc gattgaatat atttttaaaa
    21601 atgagcgctg cttgatagaa caacgggaaa tcggtattga tactaatgat tttgcggttg
    21661 ctcttaaata tgttttgcgg caagatccta atgtcatcat ggtcggcgag atgcgtgata
    21721 acgaaacaat ggaggcggcg ctgactgcgg cggaaaccgg ccacttagtt ttttctactt
    21781 tgcataccag cacggcggtc gaggcgttat ccagaattgt tagttccttt ccagcttatc
    21841 aacaaggcca aattgccacc aaattatcat tatgcttgag ggcggttatt tctcaacgtt
    21901 tattgccagc tacggctggg gggcgagtgg ctgtccggga aattatgttt aatactccgg
    21961 ccattgccaa tcttttacgc agtcagaaat tagagcagat cccctcaatt attcaaactt
    22021 cgtcacgtga aggcatgatt tcttttaatc gggcagttga acaattatta atgactaaac
    22081 agatatcgcc agaagtggcc agagcttatc agcgtggcga ttcgggggct tcttattggc
    22141 gttaatttaa ttaagacaat atttatgtgg attattttct tggttattat tgtggctttg
    22201 cttttagcca gtttgcgtca gattaatcag tatcagcgcg gcgtgctttt tactatgggt
    22261 cgctttagcc agattatgga gccaggctgg cgtttggtta ttccggtttt tcagagtttt
    22321 caaaaagtgg atatgcgggt taaggctgtt gacgtgcctg atcaacgagc cattaccaaa
    22381 gacaatgtgt cagttacagt taatgccgtt ttgtactata aggtttttga tgccgccaag
    22441 gcagtgattg aagtggagaa tttttattat gccatttcgc agtatgctca aacaacaatg
    22501 cgtaatattg ttggcgaagt tactttagac gaattattaa ccaaccgaga gaagattgct
    22561 gaacgaatta gggaaattgt tgatgtagag actgatgctt ggggattgaa agttaataat
    22621 gttgaactta aagatgtcag tttgccacca acgatggaaa gaactattgc taaacaagca
    22681 gaggcggaaa gagaaaagcg tgcagtgatt attaattccg aaggcgaaat ggcggcagca
    22741 gaaaatgtcg ccaaggcagc acagatcttg tcggcgattc ctggggcgct gcatttaaga
    22801 acactgcaat ctattaacga tatttcttcc gatcaatcta ataccatagt ttttgctgtt
    22861 ccggttgaag ctttgcgggc gctggatggt tttaaataaa aaataaagat gataacgcca
    22921 gtcaacaaaa aaattattct cagcggttta gccgttttta tcggtcttga gattattaat
    22981 tggcgggttc aatatctata ttggatcata gcgctatggt taattttatt tttagccatt
    23041 gtctggtttt ctttaagtaa ccgttcccca aaatataaat taatggtttg gttaattccg
    23101 actcttaatt tgttggcttg gtgcggtttt ttaaccattg tcaatagttc atttattcgg
    23161 caattattaa ttttaaccac cgcagccatt cagttagctt attggcgtcg cggttggaca
    23221 gcctggttgg aacaaaagcc accggcagct tggcggcgtc tggttaatgc tattaatttg
    23281 ttaactattt ggttggctgc cgccactctt tatggttggc aagccttttt agcttggccg
    23341 atttggtggt catggttatg gtggctgatt ttgtccggtc taattttatg gttagactat
    23401 caaacattag ctttgccgtt atcaactcat tggccagtgg ttttaacgca gtggctgatt
    23461 ggtggccaga tatttttggc agtttacttt ttacctagct cccacttagt cttggcttat
    23521 ttgttactaa tcgcttatta tgttgtcagc cagattggtc gcggaagttt gttaaaaact
    23581 aatactgctc gccgtttgcg ctatcagttt ttaattttaa taatattgtt agcggtggtt
    23641 ttactaaccg ctaaatggtt ttaatttaat atatattttt atgagttcga ctttgattcg
    23701 caaaaagatt tctcttagct taatttggtg gattttggcc attgttgtta atttaatttt
    23761 agctctggtg atggttaagt atttttttgc tattgagcaa gccaatttat tgacttcagc
    23821 aacgttatcg gggcaaaacg ctatcggttg gccaatggcg attaatcaaa gttcaacaac
    23881 taagcggcca gcggctattg agtataatga agcagctagt ttgcaaccga gtttatcatt
    23941 aagtttggtt aaagttttcc gtcaagcgga tcgcatcacc tcggcaccga tcaaacagga
    24001 taatctgtat aatctcaatc aaccgctagc tggcggttgg gtggcaacta gcgatggctg
    24061 ggtggttagt tcggctgatt tggataactg gtcagattta gcagtggtcg ccggtaaccg
    24121 caaggtttat aaaattgatc aaaaaactag cgatccattg accggtttat ttttctatca
    24181 tttagctggg gcagctaatt tatcacccgt taaattaggt gaagtcggtt ggagtcggcc
    24241 agggcaatta atgtataatt tagataattt gggcggtctt aaagctgtgt ggttagagcg
    24301 agtaacaact gatcaaaata ataatattat tagcagcgat attttgtggt atcgctggac
    24361 attctctagc gtttggccta gctcagctat ttttaacggt caaggcgatc tgtttggttt
    24421 agttgatagt caaggaaaga ttattccagc caattatatt aaatctggac ttaatagttt
    24481 attgtccatg ggtaatttca agcgggttgt tttaggaatt gagtattttg atttgaatcg
    24541 ttcttccact gaaaggagcg gcctgttggt caatcaggtg actgctggca gctcagcgga
    24601 taaggctggt cttaaggctg gtgatataat tttaagtatt gacgagatta atcttgatgg
    24661 tcagatcgct atcaatgaaa ttttacaagg ctacaagact ggcaatcggg cgagcttaat
    24721 tgttcagcgc gccggtcaaa atcgtcaatt aaattgtcaa ttttaaaaat atgttcagtc
    24781 gcactatttc cgccatgcca aatttatcgg gccgccaagt ttttttgcgt ctcgatttaa
    24841 atgttccgct caacggtcag gagatcgttg aagattataa gattagggca gcattaccat
    24901 caattaaggc attattagac catggagcta aaataatcat cgctagccat ttaggcgagc
    24961 cgctagttaa aggcaagctg atagttggcg ctaagacggc gctaactatt aaaccgattg
    25021 ctgctagatt agctcagctt ttagatcggc cagttaaatt tttgcctggg caaaatttgg
    25081 cagttttgcg gcagaaaatt agccgagcta ctgagaatgt tattgtttta gataatttac
    25141 gtttttggcc cggcgagtta gaaaataatc gtgactttgc caaagagttg gctagcttag
    25201 ctgattttta tgtcaatgat gcctttgcag ttagtcatcg ccaacaggcc tcagtggctg
    25261 ccattaagga tttcttacca agctttgctg ggattttgtt ggaacaagaa gttgttaatt
    25321 tatctaaagc gctcaatcca aaacagccat tgattgtcgt tttgggcggc gccaaggttg
    25381 gcagtaaagt tcacttaatc cgtcgcttag gccgtaaggc ttccaaggtt ttacttggcg
    25441 gtggtttagc taataatttt ttagcggctc aaggttatca ggtcggccaa tctttagttg
    25501 acaaggaaag cattgattta gcgcggcgtt taataaaaga actaggcaaa aaattagttt
    25561 taccaattga tgttattgtg gccgattcgt ctagtcagcc agttttgcga ttaatcaatc
    25621 aagttggcga tcaggacaga attttagata tcggtccggc gactatacaa gaatttagtc
    25681 ggcagattaa gatgggcaaa actattgttt ggaatggacc gcttggccag tttgaaaatg
    25741 agcactataa aaatggcact ttgtttatag ctagagaaat tgcttggcag tcgcgcggcg
    25801 ctgcttttgg attagttggt ggcggtgaaa ctgtggcggc gctacaaatg actcagatgg
    25861 ctggttatat tgactgggta tcaacagccg gcggggcgat gttggcattt ttaggcggtg
    25921 agccgatgcc gggtttaaaa aatattattt aatttttatt atagaaaaac tgtatggcta
    25981 aaattgttca attgaccgct cgggaaattc tagattcgcg cggcaacccc acggttgaag
    26041 ctaaagtaac cctggataat ggcttagtcg ctaaggccag tgtgccgtca ggagcctcga
    26101 caggtgttca tgaagcccat gaattgcgtg atggtgataa aaatcgttat ggcggtaaag
    26161 gagttttaca agcggtggcg aatattaaag atgttattgc gccaaaaata attggtcagg
    26221 aaattattga tcttccggaa attgatcgga taatgattga tttggatggc accgctaata
    26281 aagaaaaatt gggagccaat gctattttag cggtatcaat ggcttgcgct cgtgccggcg
    26341 ctatggctac cggtcaagaa ctatatgctt atctagccag tatttatgat ttcgcgaccg
    26401 ataattatcg tttaccaaca ccaagtttta atatttttaa tggcggccag catgctgata
    26461 ccaatgttga ttttcaagaa ttcatgattt tgccattaat taatagcaca gtagccgaga
    26521 aaattcgtct aggcgctgaa gtttttcatg ctcttggtaa ggttttgcaa gaggctggtt
    26581 tagccactaa tcttggcaat gagggtggtt atgcccccaa gatggaatat agtgagcagg
    26641 taatggaaat gattgttcaa gcgatgtcgc gggctggtta tcagccaaaa gaacaagttg
    26701 gttttggttt agatgttggt tcgtcagaat tatatgatga agccactggc tattatactt
    26761 tttcattaga taagtggcag tttacttcag cggaattgct ggctaaatat cgagaatggt
    26821 cggaaaaata tccgattatc tctattgaag atggtttggc tgaggatgat tggtcgggtt
    26881 ggcaaaattt gacagccgaa ctgggcgacc ggatgttatt aattggtgat gatttattcg
    26941 tgactcagac tgcaagattg cggcaaggaa ttgccagtca agcagctaat gcgattttaa
    27001 tcaaacttaa tcaggtggga acagtgactg aaaccataga gtgtattaaa gttgctcaac
    27061 agaataatta caagattatg atatctcatc gcagcggtga aacaaccgat gattttattg
    27121 ctgatttagc cgtcgctgtc ggcgctgatt atattaaaac cgggtcaact gcccgtggcg
    27181 agcgattagc taagtataac cgtttaatgg aaattgagct gcaattagct ggcaattttt
    27241 aggcggctat gcctgttcaa actgataaaa taaataatga ttctgcagct agttcagtag
    27301 tcttagtttt gctagacggc tggggcgtag ctccgccggg accaggtaat ttattgacta
    27361 atttagcatt gccgaacttg agtgattacc tgcaacaata tccgttggca gttttggcaa
    27421 cgccgttgat acgcaatggt tcagccaaag aagcttattg ccagattggc agcggtcagg
    27481 atagcagctt ggttcctggc ttgggtggag tagctgacca atctggttta gctgaattgt
    27541 tgtcgtcggc cggtcgtcgg caggcctatt ttactgaaac tgaaaaattg ccactagtcg
    27601 tggagtgctt agctggcgga cgacaagatc cggaaattga atatcgggtt atagccacac
    27661 cgcttaataa tgattataat gaacaggttt tagctgccag tcatcaacta attgatcagg
    27721 cggtgagcta ttatcgtcat tatcggcctg attttttaac catcaacttg gcggcttgga
    27781 gttcggtggc tagatatggt acagtggaaa gcattgccaa agcaatggtt gcaatggata
    27841 aactattgcc caaattgctt aatccgatta ttcaaactgg cgctaccata ttattaactt
    27901 cggcgtatgg tcgtttggaa agcttgattg atccgctgac ggaaaaagtt aatttcggtc
    27961 cgagcaataa tccagtacct ttcttaatta ttggtcgttt gtggccaggt cagagtttag
    28021 gggagactga aatcgctagt aacgatttaa gtgttttacc gctcagcggg tctctgcggt
    28081 caattgctcc gacaattttg gctctattag gagttgagcg gccgtcttat ctagagaagc
    28141 cgttgttacc gataaatatt aattaaaata ttatggcagt cttgttatta aatgctggat
    28201 ctacttcgtt aaagtatcaa ttgtttagtg atggcggcga ggttattaaa aggggcggtt
    28261 ctagtcgagt taaagacttt gatcaggcct tcagtcagat tatcgaagag ctggaattca
    28321 ttggcgagat aaccgctatt ggccatcgcg ttgttcatgg cggcgatcat tatcagcgtc
    28381 caaccatcat caatgatcaa gttattgatt atttagaaag tatcgttaat ttagccccac
    28441 tgcatttacc ttttaatctt ttaggtgttc gtttggctgc tgagcattgg cctaacttac
    28501 cacaagtggc ggtgtttgat acagctttct ttgctaattt gccaactgtg gctaaaacct
    28561 atgctttacc gctagatttg gctacagaaa agcattggca tcgttatggc tttcatggat
    28621 tatcgcatgc atatttaact cacgcggccg cagacgaatt gaacaaaaaa ttagaacagg
    28681 ttaatttaat tatttgtcac ttgggtggcg gcagctctat ctcggctatc caagccggta
    28741 aagcaattga tatttctttg ggttggtcgc caacggccgg tctgccgatg atgactcgtt
    28801 gtggtgattt agatcccggc ttgttattaa ccataattga ggacttgcca ggaccagtta
    28861 atgaacaaaa aattaaccaa gtgcgtgatt tattaaataa gcaatctggc ttatgtggct
    28921 tgtgcggtta cgatgatttt ttagatattt taaccgctcg ccaacatggc gaaaccaggg
    28981 cggccgccgc ttttgattat tttgtttatc gtctggtgca ttatattggg gcttattggg
    29041 caattttagg cggcaaggtt gatgcaattg ttttaagcgg cgccatcggc gctggcagtc
    29101 tagatttacg tcaggcgctg gcgaacaagt tatctggttg gtgttcagcc tcgttgctag
    29161 ctatgcccac caacgaggag ttagctatgt ttcgggagat taaaaaacta gggctataat
    29221 ttatggcgct aaagaaatta tggtatggat ccattaataa tatcggttgg gcagcggtcg
    29281 tggttggagt cgcttcattt atcagccgat ttcttggtgt ttttcgtgac catattttag
    29341 ccgggcagtt tggtgccggc gatgaattgg atatttatta tgccgctttt cgcgtgcctg
    29401 atttgatttt taatttgttg gttcttggtg ctttgtccgc cggctttatt ccaatttttg
    29461 ttagcttatt aaataattat gatgagccgg cgaataatca tgatgcttgg cgttttgcta
    29521 atctagttat aagttcatta atagttattt tgcttatttt atcagtggtt ggtattattt
    29581 tagcaccgca attattaagg ttattaactc cgggtttttc cgctgataag ctggcgacaa
    29641 ccgttaagct aagtcggttg atgtttttgt cgcctttatt attaggtatt tccggagttg
    29701 tcggcggtat tttgcaatct tttaagaatt tttttgctta ttcgctggcg ccgatttttt
    29761 ataacttagg cattattact ggtgctttat ttttagtgcc tcgatttgga ttaatgggtt
    29821 tagcctgggg ggtggtcatc ggcgctgcct tgcatttatt agttcaatta ccgactttgt
    29881 ggcgcttagg ttttcgtcct aaaccattat tagattggca tgaccaagct ttaaaacaga
    29941 ttggtcggat tatgattccg cggacgatgg gtctggccat tagccagctt aacttagtgg
    30001 taatgactat tttagcttcg gcattaacta gcggatcgtt agcgattttt aatttagcta
    30061 ataatctgca gtcttttcca gttggtattt tcggtttatc atttgccatt gccgcttttc
    30121 caaccatttc agctctggtt gatcagccgg ataaattaaa agccaactta acggttacta
    30181 ttcgtcaaac tttattttta gttataccgg cgacgattat tattttagct ttgcgggcac
    30241 aaattattcg ggtaatttta ggttcgggtc gttttgattg gcaagatact atcttaacga
    30301 tgcggacttt gagttatttt ggcttatcta tttttgccca atctctaacc gccattttag
    30361 ttagagtatt ttatgcttat aaagattcgg cgactccgtt ttggatcagt ttgctggcag
    30421 ccgtagtcaa tattggtttg gcctggccat ggagtcgaca actgggcgtt gccggtttag
    30481 ctttagcttt ttccgttagc aatattgtta attttatttt gctgtggtgg gcgctgcggc
    30541 gcaagggtaa tattttagat aatttaacta tcggtatcgg tagtttgaaa atggtcttgg
    30601 cggccgctat tgcttggccg gctatttttg cggctaagag attaattgcc ccgttagttg
    30661 atatggatac tttttggggc attactagtc aattaattgg cgcagcaaca gtcgggctag
    30721 cggtttattt gttcatctgt ttgttattac gttgtccgga agccaagcag ttgtggcaaa
    30781 tagttaatcg aagattacct ggcggccata aaacagtagt tgatgccgat gaggcgcgag
    30841 gtatctagat aagcagatag ttttattagt tgacagtcaa gattttgctt gacttttttc
    30901 gtagatagtt gttagataaa gtattcaatt gttctttaac aaaaaaataa aaaaaggagg
    30961 tgtcatatca tggcacagca tgtttcctta attttctttc gtcgcatacc atgcgatccc
    31021 cagatcgacg aaagtggttg ttgtgatatt gaggtgttgc tctcccagcg agcagcattt
    31081 cagtttagtg atgcgtcgcc caaccccaat ctttctttcg gggaatgcag attttccccg
    31141 gcggaaaatt ggaaaaggac gatgctagtc ctttgcaagg agcttatcgt gagctccgtg
    31201 aagaaggcgg ccagcgcctg gccgactggc tgaagtcaat cctccaggtt agccctgaga
    31261 ggttgatgga gatcaacaac ttttgttttg ctgttgaggt ccggccggat gatgatctgg
    31321 ccggtgaaaa ggaatttgtc aatcttatca aactagcggc cgagcatagc tcggtcgact
    31381 ggtactccaa tttagaggtt gccggatttc aagatctggc tccctataaa gatggggtgc
    31441 ctaaaggtgt aaaggccatc tttgccgatg aggcaggatg gattaaacaa ctgactgagt
    31501 taatttcccg ggaggggtga tggaaacggt tgttttggcg ttagccaaaa aatcaactat
    31561 cccttgaaaa gggaggttga aaacctggct aatgagggtt acccaatttt cacccagcat
    31621 gacttaggtt caatcttaga acttaagtca gggaacacgc taattatagc ctgccaatct
    31681 aatgatcacg tctcaacatt ggctcttgtg gagcagtttg ctcaagcggc caaaataaac
    31741 aaatgggaag tggttaaatt ggtggccgcg cgccagcaca tctggcgctg tgctagagat
    31801 ctgaaaaagg tcttgccgga aatttgtctt gacaaaattc cggtcaaggt cgcccattgc
    31861 ccagacattc aggcatgggt aacctcgcca ctaaggtggt gggtgagaga gataataatt
    31921 aggctcctgc catggggcct atatcgccgc gtatgcggct aatgcccctg agatttaaag
    31981 gggtttcatc gcacaaatat acaccaaagg aggatatatc gatttaacca cagaccccca
    32041 atttgtccaa taaaaaagcg ccaacggcgc tggcgtcagt ccatcaatta actgtttaat
    32101 aacagatgga cgacgctcta aaactattca tggacctgat tgggggtcct tttttatttt
    32161 taaattttat taactagcca ttgacttaag ttgggccgtt tagcataatg aaagcataac
    32221 ttacgggtat gcagaacatc cgcaattttt gtattatcgc ccatattgat cacggtaagt
    32281 cgactctagc tgaccgtctt ttagagttta ccggtaccat tgatccgcgc aagatgacca
    32341 atcaagtatt ggatcgtttg gacttggagc gcgaacgagg gattactatc aaattaacac
    32401 cagtacgcat gaagtatcag ggctatgagc ttaatttgat tgatacgccc ggtcatgttg
    32461 attttagcta cgaagtttcg cgcagcttag cggcagttga gggggcgatt ttactggttg
    32521 atgctactca aggtatccag gctcaaactc tagccaattt gtatttggct attgagcaag
    32581 atttggcgat tattccagtg attaataaaa tagatctacc agctgctgac gtggacaaaa
    32641 ccaaagccga aattgttaaa cttttgggtt gttcagccga agaaatcatt tgtgcctcag
    32701 gcaagactgg cactggcgtg cccgatattt tagagcgagt tatcaaggtt gtaccagcgc
    32761 cgtcaggaca accagatcag ccactgagag ctctaatttt tgattccaat tatgatgaat
    32821 atcggggagt ggtcgcttat attcgagtag ttgatggggc gattaagaag gcggataaaa
    32881 ttaatttaat ggccactcag gccgaaagtg aagctcttga tattggctat tttgatccta
    32941 attatcattc cagcggccag ttaaatgctg gtgaaatcgg ttatattatt actaattttc
    33001 gtgaggttgg tgaatgccgg gttggtgata cagtgacatg gtccagaact attggtcaaa
    33061 ccgtgccgct caagggttac actgaagttc gaccgatggt ttttgccggt atttttccga
    33121 aagaaggtaa tgaatttgag gatttgcgag aagcgatggg ccgtttaaaa ttaaatgatg
    33181 ccgctttagt ttatgaacca gaacaatcgc aagctttggg ttttggtttt cgttgcgggt
    33241 ttttaggact acttcattta gaaattttcc aagaacgtct acgccgtgag ttcaatcttg
    33301 atttaatcgt tactgtgcct agcgtggctt accgcttacg ccgtaaaaat agcagcgaaa
    33361 tagaaataat tcgcagtccg cagcaattac ctagcgccgg tgatattgat ctgattgagg
    33421 agccgtgggt caatttagat attgtcactc ccaaagatta tctgggggca gttatgaaac
    33481 tagcgcaaga aaagcgaggt atctatcgca atactgaata cttggatgaa accagagttg
    33541 tcttgcacta tgaaatgcca atgagcatga ttttgaccga tttttatgat aaattgaaaa
    33601 gttcaacttc cggctacggg tcaattaatt atgaaatatt ggattatcgt caagccgatg
    33661 tggtccgttt agatatttta gtagcggaag atatgattga atcgctgtca atgattgttt
    33721 atcgcgacga agctttccgg cgcggtaaag aagttgtgac gacgctgaaa aatactttgc
    33781 cgcggcaaat gttcgtgata aaattgcagg cagcggtagg cggtcaaatt gtggccggtg
    33841 aacggatttc tgctttccgt aaagatgtaa ccgccaagct ttatggcggt gatgtgaccc
    33901 gtaagcgtaa attgttagaa aaacaacgcc agggtaagaa aaagatgatg gcaatgggtc
    33961 gtggcagcgt ggatattcct agtgagactt ttgttaaatt attaaagcgt taatttttag
    34021 aatttatata tgagtaacaa aagaaacaaa aagaaaaaga taaccaagcg acaaaagatt
    34081 agaatagcct ggataattat tagtgctatc gtcattattt ccatggtggc tgggactata
    34141 atcatgagcc tttaatatgg cggctgagcg cctctggcag gtggcgccgg ttttggttaa
    34201 tcgtaatccc aaattggccg agctgaaact gccggagatc attgaacaaa tcttatataa
    34261 tcgcggctta actgatccca tggcggtccg ccagtttttg ggtttagaat cgtttgattg
    34321 ctcgccagga cgattgagcc aattagaagc cgccgtggag ttgattgttg agcatattaa
    34381 ggccggtcat ttaatggtgg tttatggcga ttatgacgct gatggcgtaa cggccagcac
    34441 tattctattg gaaactttga caaccttgaa agccaaggcc agaatttggt tgccggatcg
    34501 gattactgaa ggttatggct taaataagcc agccattgat gaattagcgg ccgctaacgc
    34561 taaattaatc attactgttg acactggtat tcgtaataaa gaagaaatag cttatgctaa
    34621 aactaagggg ttagacataa ttgtgactga ccaccatcgt gaatcgccgg atccagctga
    34681 ttggccagat tgtttaatga ttaatccgac tatggctaaa gaagattacc cctgtaaatt
    34741 tttagctggg gttggtgtgg cctttaaaat ggtgcaggca ttgttacggc ggtcaacttt
    34801 agatagttct caaaagcaga ttttagaacg ccgctgtctt gatttagtcg ctttaggaac
    34861 gattgctgat tgtgtgccgc tagccggtga aaatcgttta ttggttaagc agggcattga
    34921 agtgctaaat gaatggcggc gagtcggctt acgtgaatta gcccgagtag ctcaaattac
    34981 cggcgaagtt aatgaatgga atattagctg gcaattaacg ccgcggatta atgtcgccgg
    35041 tcgtctggat cacgctaata ctgcttatcg tttactcact accaccgatc aagctgaggc
    35101 ggctcagttg gctgaggatt taaaccaaaa aaatttagat cggcaacaaa agactcagga
    35161 aatagtggat ttttgtttaa acgacttatt agaattacaa gctgaggata atttattgat
    35221 ttttattagt ccgcgccaag aaccgtggcc ggaaggtatt attggtttag tcgccggccg
    35281 cttgtcggaa aaaacggcca agccggtgtt agtgattact aaaagcgaga atggttataa
    35341 gggatcgggt cgcagtaatg tggagaattt tagcttgatc gcccaattgg aagaagtagc
    35401 cagtgaccta gatcggtatg gcggccatgc cggcgcctgc ggctttagtt taactgtcca
    35461 acagttaccc aattttttaa ccaagattaa gaagttagcg gcggctaaat taaccggtct
    35521 ggatttacgg ccaaagctat ttattgacgg tgaattgccg gccgatcaag tggatttatc
    35581 actagcggaa tggctagaaa aattagcccc ttttggtcaa ggcaatcagc aaccaagatt
    35641 cgtggcctat cggcagacga ttcgcgatat taatacgatg ggagccaata agcagcacat
    35701 taagttgcgc ttgggcaatt attgggcact agcttttggt caagctgaag attatttagc
    35761 gcaagacctg cggccgggcc gccaaataga tttagtttat agtctggata ttaaccgttg
    35821 gcaaggccaa gccgaaccac agctaaagat tattgattta cggccctcaa ccgataagta
    35881 aaataagcaa ttatatatgg tcaaaaaatt tgttcatcaa tatttaatta gctataccga
    35941 tggcggcgcc cgcggtaatc ccgggccggc cggtattggt gtagttttgc agaataaaga
    36001 cggcgaaacg atcggcgagt ggtcggagtt tttaggtatt gccactaata atcaagccga
    36061 gtataaggct ttgttattag ctcttaaaca agcggttgct ttgggagcaa ttacattaga
    36121 atgccgctta gatagtgagc tggtggttaa gcagctcaag ggcgagtata aggtcaaaca
    36181 tcccgagctc aaacctttgt ttgagcaagc caaaagcttg atttctcaga ttaaaaatgt
    36241 tagttttaag catattcctc gcgagctgaa taaacaagcc gatcagttgg ccaatcgggc
    36301 catggatcag ggcggggatt tttaggacgc atagctcagt tggttagagc gtacggttca
    36361 catccgtaag gtcacaggtt caaatcctgt tgcgtccacc agttaagcaa aaagaaaagc
    36421 gacgctcaag acgtcatttt gataatgtta tagtgcaagg ctaacggcct tttttatttg
    36481 actaatactc ttagggcggt ataatagaca ttagaccaga ccgccagcgg gcagccgcta
    36541 tttagcttac taaatagagg aaagtccgga ctcccgcggc gctaagcgcc gtgtcgcgat
    36601 aacgggtaac gcccgccgcg tttgccgtga ggcagacgta gggaaagtgc cacagagaca
    36661 atactagcga ttatatcgtc aaaggtgaaa agtcagttgg tgttgcggct tgccgtagct
    36721 acagctgtcg ctaatcagta atgattagtc gtggtaaacc ctatctggag caagagcaaa
    36781 cttttggttc gccaaaagac caagtagctc gctggagccg tcaagcaatt gacggccaag
    36841 ataaatggct gccgtcctct cggggataca gaatccggct tatagctggc cgtcggtcta
    36901 aaatttaaaa tttatgaaac gcagtgaatt atttttttct tttattttgg taccggttga
    36961 ttttttaatg gtttttttgg ccggtatttc cgcttatcat ctgcgggcta gcgagatggc
    37021 cactggaatc aggccgattt tatataattt gcctttcggt gagtttttat ggtcgctggt
    37081 tgtcgtggcg gcagcttggt taattatttt tgcgattgcc ggtttatacc gagcccgcgg
    37141 tattaagcgt tttgaggagt tgagtcaagt tttttttgct tgttttgccg gttttggttt
    37201 gattgttatc tatattttct tttttcggga atggttttct tcgcggttta ttatcttagt
    37261 tggctggtta ttggcctggg cctatgttag tttagcgcga ttaattgttt gttttattca
    37321 aaaacaatta tttcgttatg gtatcggcgt ccatcgagtc gtaattgtcg gccgcggcgc
    37381 ttcggcggat aaattaatca aagaatggtt cagtcatcgg tcgcgaggtt atcaggtggt
    37441 taagcattat gatgatttta gcttggagat tgctcaggaa ctagcggact atttgcaagt
    37501 caaagaaatt gatgaaatta ttcaagccga tcctaattta agtaaagctg aagtggttcg
    37561 gctctatgat tttgccgatg aacatcactt gacttttaaa tatgttgctg atttgttgga
    37621 cactaaagtt ttaaaaaccg aagttgatga agtggccggc ataccagtgg tagaagttaa
    37681 gaaaacgacc ctggatggtt gggggcggat tatcaagcgt ttatttgata ttgttgtttc
    37741 tggtatttta attattattt taagtccagt ttatttactt accgctattg ccattaaatt
    37801 ggattcggcc gggccggtct tttttagccg ccttgatgac ggttcaccgt tgtgccgaat
    37861 cggtcaagcc ggccgtaaat tccgttattt aaaatttcgt tcaatggcgc cgggcaccga
    37921 tagtctgcgt tacactgagt tagctgatcg caatattcgt gatgacggtc cgatggttaa
    37981 gattaaagac gatccgcgga ttactagagt aggcaagttt atccgtcgtt ttagtattga
    38041 tgaattacca gaattatttt tagtctttat cggttcaatg agcttggttg gaccgcggcc
    38101 gcatttaccg gaggaggtgg ccaaatatca gcggcatcac aaaaaagtgc tgactattaa
    38161 gccgggaatt actggtttgg cccaagtttc cggccgctct gatttaagtt ttgaagaaga
    38221 ggttaagtta gatgtttatt atattgagaa ttggtcgttg gctcgagata ttcgtatttt
    38281 attaagaact ccgatggccg ttttgcgtca tcgtcaagcc gcttaattat gaaaatcgct
    38341 ttaattcatg atcatttagc ccaagacggc ggtgccgaaa aggtattaaa ggtgttggct
    38401 gagatgtatc cacaggccga tatttatacc ttgctttacg aacaaaagca cgccgataaa
    38461 tatttttccg gccggaccat caaaacgtct tttattcaac gaattcctgg tggagtcaaa
    38521 cattatcgct ggtttttgcc atggatgccg atggcggttg agttttttga tctatccgac
    38581 tacgatttag ttatttctga cgccagcgct tttgctaaag gcgtgattac cggtcccgaa
    38641 gtaccgcata tctgttattg ccatacgccg actcgttatt tgtgggatta tactcatcgt
    38701 tatattgctg agcttagata taataagttt ttaaaaaaaa ttgtttcctt gtttttgaat
    38761 tatctgcggc tctgggatcg gcaatcggcg gatcgggttg attattttgt ggccaattct
    38821 aaattggtgc aaaaaagaat taagaaatac tatggccgcg atagccaagt tgtttatcca
    38881 ccagttgatg tcgatagctt taaagtcgtt gatcaaccaa gtgattattt tttaatcggt
    38941 ggtcgagtgg tagtctacaa acggtttgat ttagtagtgg aagcttttaa agaattgggc
    39001 tggccgatta agatttttgg cgatggtgtt gatttgggca gattaaaaaa gatggcggct
    39061 ggggccagca atattgaatt tttggggcga gttgatgaag ctaaaaaaat tgaattatat
    39121 caaaattgcc gggctttttt aaatccacaa gaggaggatt tcggtataac tgtagttgag
    39181 gccatggcct gcggtcggcc agtcattgct tatgccaagg gtggggcgtt ggaaacggtg
    39241 gttgacggtc agaccggtgt tttttttcaa gagcaaactg ttaaagcatt agtgtcggct
    39301 ttgcgtcgct ttgatcagtt gacatttaat ccagccacta ttcgtcaacg agctgaacta
    39361 ttttcccggt caaattttga acagcaattt aaagatgttg ttaataagtc gctaactgat
    39421 tttcagcagc gctaaataat atgcggattg ccgttgacgc taggccgctg ctagctaaac
    39481 aaatcagtgg tattcccgaa tatactggac ggctgattga tgctttagta accgctcatc
    39541 ccgaagcaga ctggcggctt ttttatagtg gttggcatca acggcccgat aattggccgg
    39601 ctctcaagga gaaaccaaat attagttggt gtcggctaaa atgtcccaat aaattaatta
    39661 atagtgcggc ctggttattt gaccggccgc gtttggataa atattgttca gctgatgtct
    39721 ggttattgcc gcatttcaat tttacaccgc taactggtca gataccgact atattaacaa
    39781 ttcatgattt atcttttttg cgtcagccag agtttttttc ttggcgccgc cgcttttggc
    39841 atgccagtct gcacttgcct agattaatta ataaagttaa taggattgtg gcgatttctg
    39901 aaaatactaa gcgtgatttg atcgagttaa tggctgtgcc cgagaaaaaa attacagtta
    39961 tttattcggc ggccaatccg gcttttcggc cgattatgtc cgacgaccag cgtttaacta
    40021 attgccgtca gcaatataat ttgccaaagc gctttatctt atcgctagcg acctgtgagc
    40081 cgcgcaagaa tttggtcaat attattcgag cttatgataa tttacgtcag cgttgcccca
    40141 gcttggctga ttgttcattg gtgattgccg gtggtcaagg ttggaaacag ggggcggccc
    40201 gaatagcttg gcgacaagca gccaatcgtc aagatattta ttggctcggt tatttgccgg
    40261 ctgatgattt accaattgtt tataatttgg ctagcctgat tgtttatcca tctttttatg
    40321 aaggttttgg cttgccggtt ttagaggcca tggcctgcgg tcggccggtc attactgccg
    40381 ctaccacggc tttgcccgaa gtggccggca ccgctgctct attaatagat ccggctgata
    40441 gtcaggcctt atcgcgagca atggagcaga ttctgactaa ccggcaattg gctgagcagc
    40501 tagctcaggc tggccggcaa caagctagtc aatttagctg gtcaaaaaca gccgaggagt
    40561 attggaaact cttgagattt agcttgtaga ctaaatgaca gtgccgactc tttttgttat
    40621 actaaaatta ttattaagcc gttaattatt tccccaatgt ctaaccaacc ctttgtccat
    40681 ctgcatgttc atagccatta ttccttgctt gatggtatgg gcaagattga tgaattagtg
    40741 gaggcggcta aagctgatgg agccacagcc gtggctttaa cggatcatgg ggtcatgtat
    40801 ggcgtcgtgg agttttatca gaaatgtcag aaggccggta ttaagccgat tattggggta
    40861 gaagcctatt taactcctgg ttcgcgtcac gataagaata gtcagattga gcgcagctat
    40921 catttaattt tgttagccaa agatcaggtt ggttacaata atctattaaa attggttagc
    40981 attgctcacc tggaaggttt ttattataag ccgcggattg attgggagtg cttgcaaaaa
    41041 tatagccagg gcttgattgc tatgagcgct tgtttagctg gcgagatccc gcggttaatt
    41101 atgtccggca aaaaagagca agccaaaaag cgggcgctag agtataatca attatttggt
    41161 caaggcaatt attatttaga attgcaagac catccacatt tacccgagca agagaaggtt
    41221 aatcaggaat taattaaatt gtcgcaagca actggcatcc cgctggtggc gactaatgat
    41281 gttcattata ttagagcctc tgatcgcgat gttcaagatg ttttattgtg tttgcaaacc
    41341 aagcacaaaa agaatgatgc cggccgaatg aatatgcttg aggatgatta ttcttttcgg
    41401 cctagtgccg aaatggcggc tgcctttgct gcggttccag aagcgattaa taatactgtt
    41461 aaaattgctg atcaatgcaa cttagagcta gcgatgggtc aagttctgtt gccgcgcttt
    41521 actgtaccta atgacctcac tgcccaagat tatttgcgtc aattatgctt agacggatta
    41581 gttaagcgtt atggccaaac ttatgaagaa atagagccgc tttatcgaga acggatggac
    41641 tatgagttgg agattattgc taaaatgggc tttcctgatt attttttaat cgttgctgat
    41701 tttatcaatt gggctaaaaa taatgcaatt gtcgtcggtc cgggtcgtgg cagtgccgcc
    41761 ggttcattgg tttgctattt gattggcatt accgagctat gccccatcgc ctatgatttg
    41821 ttatttgaaa gatttttaaa tccggatcgc atttctatgc cggatattga cactgatttt
    41881 gctgatattc gtcgtgaaga tgtcattgaa tatgtttcag aaaaatatgg ccgcgatcat
    41941 gtttcgcaaa ttattacttt tggaaccatg gcggcgcggg cggcgattcg tgatgttgga
    42001 cgggtgttgg atgccagcta tgatttttgc gataaactag ccaagatggt gccgatgttt
    42061 atgaaattgc cagaagcctt agagcaagtg gcagaactta aacagattta tcagcaagat
    42121 ccgcaagcta aacagatttt ggattatgct cagcggctgg agggatgtgc tcgacatacc
    42181 tcaacgcacg cttgcggcgt tttgatcact aaagatccgc taactgaata tacccctatc
    42241 cagtatgctt catcaaccga tcgaacagtt gtttgccaat attccctgca tccagttgaa
    42301 gatttaggct tgttaaagat ggactttttg ggtctgaaaa atttgaccat tattgagtca
    42361 gccattcgta tcatcaaggc tactaaaggg atagaaattg atattaatca gattccgctt
    42421 gatgatcagg agacttttgg tctctttcag cgcggcgaga cgaccggggt ttttcaattt
    42481 gaatcgtccg gcatgaagcg ttatttgcgc gaccttaagc cgaccaattt ggaagatata
    42541 attgccatgg tcgccctgta tcgtcccggt ccgatggaat ggattcctga ttatatcgcc
    42601 ggcaaacatg gccgtaaaaa accgcaatac ctgcatccaa aattggaacc gattttggct
    42661 aaaacctacg gggtagctat ttatcaggaa caagttatgc agattgcccg cagtctagct
    42721 ggctttacca tgggtcaagc cgatgttttg cgtaaagcca tgggtaagaa aattgctaaa
    42781 ctgctagctg agcaaaaaga aaaattcatt gccggctgcg tcaataatgg cgttagtcag
    42841 actttggctg aaaaagtgtt ttcttttatt gagcctttcg ctggctatgg ttttaatcgt
    42901 tctcatgccg cttgctatgc tatggtggct taccagacgg cttatttgaa agctaagtat
    42961 ccgacagctt ttatggcggc gctgttagcg gccgatcaac aaaatagcga tcgggtggcg
    43021 attgaaattg atgaatgtcg gcacatgggt atcgaggtct gtcagccgga tattaaccag
    43081 tcctttgatt ctttcactgt cgtcaacgag gccctggtgg ataaaccgac gattcgtttc
    43141 agtttgttgg ctattaaaaa tgtcggtgaa catattgccg aggtcattaa agatgaacgc
    43201 aagactaacg gtcattatca agatatagct gattttttgc ggcgaattac tgataaagat
    43261 ttaaatcgta aatcattgga aagtttgatt aaggctggcg cttttgacgc ttggcatgat
    43321 cgccagcaat tagtagataa tttggatatt ttattaaaat ataacaaaga ttctaaccag
    43381 aatcgtttaa gcggtcagaa tagtttgttt gctggagtta gcggtgtcga gcatcagccg
    43441 ttggttttgc ggtcagcgcc accagttaat cgtcagacca agttaggctg ggagcgggaa
    43501 ttattgggtt tgtatattac cgaacatcct tttgtggatt ttaagaaaca gctaggctca
    43561 gcgctgacgc cgattaaaga tttagccgct cttaataatg agcaaaatgt tacaatcggc
    43621 ggcatggtgg ttagcagtaa aaaaataatg acgcgagcta atcaatcaat gctgtttgtt
    43681 aaattggaag atgaaaccgg cagcaccgag gtaattgttt ttccaaccct attggcaacc
    43741 agcggtgaag tttggcaaga tggccaagcg attttgtgcc aagggacaat ttccgataaa
    43801 gataatgaac gaaaaatttt agctgacaag gcaatttatt taacgctgga taatttaact
    43861 gatcaattgg caacttttcg tgagttgcct ggacagaaac gacgggctaa atcaggcaat
    43921 tggtcggggc cagtggtagt caactcagcc gatcggtcgg caacgaaaga aacagctaat
    43981 agcttagctg aacaatttga tttaatttta aatcaagaat ttgatcaaga tcaattggtt
    44041 cagttaaaag aaattttatt aaaatatccc gggccgagcc aagtatattt agaaattaat
    44101 ctaaacaata ataaacaaag gatagcgact gggtttaaag tcaatcgtca gcctaaactt
    44161 ttagcagaac tcatggctaa atttgccgga atggttaaat tttagctata ataaaaaaag
    44221 atacttttat taaaatcttt ttatgcctgt aaaaaaaact aagaccactg ccgccaaggc
    44281 tggtagtggt tcaactaaac cggttgctcg tcgttcggcc ggtcgtccgc cgaaagcacg
    44341 accagcgcgg ccatcagtcc ggcggtcaac taaagcgcag ccggcagccg ccaaaaaagt
    44401 tgttcgtcgt cagccgccag ttcaacccaa agtagtggtt gtcactaatc agccgctgat
    44461 tactaatgaa gaaatggctg tctggcaaac tgtccgccag gaaaatgatc aaccggaaca
    44521 gttggcggat aacgcagcct tgtcatcaaa tccgcctagt caaataccgg ttgacgaacc
    44581 atttattaat caatggacga cggttaatag ttatgtgcca actgaatctg accagcgctt
    44641 cggtcgcctt tatcgtcggt tagcactgtt ttttatcggc ttaattacta ttgtcgtaat
    44701 ggtaatgttt tattattcat taatccgggt taaaataact atctatccca agagtgatca
    44761 gccagcccgg caacagacaa ttgttttaac tgatactgtg ccagccaccg atggagcggt
    44821 accggccaaa ttcttgcaaa tcaatagtga atatcggcaa acatttagag cgactggtca
    44881 aagtcagact actggttcag ggaaattagt cggtcaggtt actttggtca atacaacagc
    44941 taaagatcag ccattgcgag cgactactag attattaacc gccaatggcg tgctatatcg
    45001 tttgcgaaat tttgtgactg tgccggccgg tggtcagatt attgctgagg cctatgctga
    45061 tcaagtaaag acggagaacg ccgtagcggc tgccaccaaa tttaccatac caggtctgtg
    45121 gtctggcttg caggacaaga tttatgccgt ggctaacaag aatattgatt atcaaagcga
    45181 tagccaggcg atagttctgg cgactgactt ggaacaggca atcagtcaag ctaagcaagc
    45241 cgccgcagct caggccaaaa atcagctcga tcaacaggct ggcggtgaag ctttggttta
    45301 ttggttggat gatagccaat ggtcaattag tagtgatgct caggctggcg atcaaaaagc
    45361 cgagttcact attactgtca aagcggcagc tggcggggct atagtttctg ctgaagcaat
    45421 taaggaggtg cttaatcaag ctgatgatgc tatcaagatt aatgaacaat cattaactta
    45481 tcagttatca gcttggcaac cacagcaaca tcaggctcag ttaaatgtca gctataaggt
    45541 tgaattagtt agtgatcaag ctgatcagtt agttgataaa cgggcgctgg tcggtctcaa
    45601 tcgccagcag ttggacactt atttgtctgg tcagcccaat gtggccagct ataaagttat
    45661 ttggcagcct ggctttttaa gcaaagtgcc gggcacagat aaaattcaat tacagcttgg
    45721 acgttagttt tatttcggtt caaatattaa aaaacagctg tcaatcgcca gctgtttttt
    45781 aataacggaa atgttgataa gctaaatatc tagattggta acttttttag cataagtctg
    45841 gataaagttt ttgcgaggag ccacttcgtt gcccatcagc atttcaaaaa tttcattggc
    45901 catagcggcg tcttcaacct caacttggcg taaaacacga ttttctgggt tcatagttgt
    45961 ggaccataat tgttctgggt tcatttcacc caaaccttta taacgctgaa tgtgtaattt
    46021 tgtcggcgtc ttggcgtttt taccgggttt gttattttgt tcagggctgt cgttgtcaat
    46081 acttggttcg ttttgttcag aattatcatc ttcattttgg ttatcattaa tctccactac
    46141 ggcggatgtg ccgccaagct ctttaactat tttatctttt tcttcatcag aataggcata
    46201 gcggacagtt gttccttttt taatttgata aagtggtggt tgggcaatgt aaaggtgacc
    46261 attggttact agcgggttaa agtggcggaa aaatagggtt agcagcaaag ttctaatatg
    46321 agcgccgtca acatcagcat cagtcataat tacaatgcgg tggtagcgca aacgactaat
    46381 atcaaattga tcatcaatat tggtacctag agcaataacc atgtttttta tttcattgtt
    46441 ttccagcatt ttatctaagc gcgctcgctc gacattaaga attttgccgc gtagtggtaa
    46501 aatagcctga aaacgacggt cgcgaccttg tttagcgctg ccgccggcgc tatcgccctc
    46561 aacaataaat aattcgcatt cttcagcttt acggctagaa cagtcagcta gcttgccggg
    46621 taaagtagcg ctatctaggg cgcttttgcg taagatagca gtacgagcag tgcgagcggc
    46681 gatgcgagct tgagctgata gcaaacattt gccgattatt tgttcagctt ctttcgggtg
    46741 ttcttcaagg aaagtgctta aggtttcact gcaaacttgt tcaacataac ctttaatttc
    46801 ggcattaccc aatttaccct tagtttggcc ttcaaattgc gggctcggta atttaacact
    46861 aataatagcg actaagcctt cgcgaacatc atcgccagtc agattatcat ctttttcttt
    46921 taaaatattt ttggacctag cataattatt cagaactctg gtttgcgcgc tgcggaagcc
    46981 gattaaatga gtgccgcctt ccatattgat aatattatta gcgaaggcca gaacggtttc
    47041 attataacca tcgttatatt gcagcgccaa ttcaacttta acattatcta gttctttttc
    47101 agcataaaag acggtttcat ttttaacctg attgttacga ttaagacttt taatatagga
    47161 cttgataccg ccttcaaagt aaaaaacata gtctttggaa cgatgtccgg gtcggcgatc
    47221 gtgtagttta accgttacgc ctttagtgag ataagtttgc tgacggaaat gatcaagaat
    47281 ggtgccccat ttgaattcag tagtatcaaa aatagtttca tcgggccgga aggtgatagt
    47341 agtgccgtgt tgattattat tggctttgcc gacttttttg gttggtccgc ttggcttacc
    47401 gcattggtat tcctgttccc aaatggcgcc atcgcgataa acggtggctt tcaaataaga
    47461 acttaaagca ttaactaccg aaacgccgac accatgcaaa ccacctgata ttttataacc
    47521 gctgccgcca aatttaccac cagcgtgcag cttggtcata accgtttcta gggctgagac
    47581 gccggtagtt ttatgaatat ctacgggaat gccgcgaccg ttatcagcca cttcgactag
    47641 accatctggt aaaagagtaa tttctaccga atcggcataa ttagccatta cttcatcaaa
    47701 acaattatca gccacttccc agattaagtg atgaagaccg gtggccgagg ttgatccgat
    47761 atacataccg ggacgtttgc gaaccggctc taaaccttca aggacggtga tggcttcagc
    47821 gctatactca gcagtagctt gttggaaatt cttgttcata gctgttttta cataaaaaat
    47881 atagccgcat aggctaagtg ctaacttaag tattttagca agaaaattga tgtctggcaa
    47941 gctgttggct ggagttttca gcttgttttt ccagtggtta attggtacaa taatacagcc
    48001 atcgtttagc ggttattatt aaaattatct tcgtgaaaaa agctttattt agtaataatt
    48061 tacccgagga cacaaccatt agccagtcac tggctaagcg tcattcgtcg tccggtaatt
    48121 ggttatggat tgcttcagct atattatcag tcgtattcat tattattatt tcccgactga
    48181 ttgtagtttg gggacagcag gccaaggtta aaaatcaatc tcagtcagcg attaacccta
    48241 atcaaatcac cgagcagcca acattacagt ttggccaaga tgctgatccg gggacgatag
    48301 ttcgccagga tccagatttt agagcggacc aattggtttt cagccaaatt tatcaagctc
    48361 caaccactag ctggtcaaca gctattttag ccaaccaagc tcaagcagta aaattaccgc
    48421 tcaatgtaaa gaaccaggcc gctaattatt atgagatacg ccgacgctat aatctggaac
    48481 cgctgattaa gaaaataaac gagcatggtt cggtaattat tgataatcca tttggtcaac
    48541 ccgagcagga tttttttagt ttttatcaag cggcgcgcca agataaatgg ccggttatta
    48601 ttaccaatga tgccattttt tattattatc agaataatct taaagaagtt tataaagccc
    48661 tagaagctgg tctttatgat ggtctgtggc aaattagtct gaacttattt gattcagcca
    48721 atcggcgtta tcaagcggca gttagtcagg cggttgatgg taacgatccg ttattggaag
    48781 cttatcgtct caatgtccag tattgggcag taaccttaaa tttattagtg cccaagaata
    48841 atcaaatcaa ttatgataat ctagcggcgg ccaccacggc gtttcagact ggcgacgatc
    48901 aaatatataa aatgccaact ttaagtagtg atttaaagca ggaagttgaa caagaggtgg
    48961 cgttgattca agcagcggat ggtccaaact tatcgccaat ttttggtcag ctgtttgatt
    49021 atcgattgtt taagccagac gccagctatc aatcgggtgg tcgattggct aacttctctt
    49081 tagctagtca gtggcttggt cgaccatggc cggtttatta ccgtaatcaa gattgtcctg
    49141 attgccaact tgatgtcgat gattggcgaa tggatttttt ggcagctaat attttagctc
    49201 gtgatttgtc cagcaatcag gaggtaaaaa attactgggc caaggtctat aagactatta
    49261 gttattttgc cggcttgcgt gacgatttaa cctatattca ttatgtaaaa gctcaacaag
    49321 atgttttggc cggccaatct ttggaacaag tggctcgcct tgatgcgtca gctcgggcgg
    49381 cggtcttaga taagctgcaa gcggctattt taaaaaattc tttttccctg gccgagggcg
    49441 gccatgatcg ccgtcaatta aaagagcgtc ccaatatcgg tctgcgagtt ttgcaggagc
    49501 gatattggcc tggacaaaat attaattgga ccaagattgg ttctcagttg gacaattatc
    49561 tcaaccaaca gtcagctaaa gattggcaag ctaatcaatt ttggtctagc gtcgggatac
    49621 ttaaagattg gttagctaag attttagtgg ttgataagtc gtggattcgt ccagcttggt
    49681 ggcgagagca acagctggcg ctggctaagt tctacttgat taattggcaa ttaccggccg
    49741 atcaatgggc attagtttta ccgagcagcg gattagcgca aaatactgtt acggcggaca
    49801 gtttaattgc cattgatact agcggcttac cggcgattga gcggcagatt gccaccgcca
    49861 aaatgttacg tctcgcttta gctaaactcc aggttagcaa cctactatca gaacagcgtc
    49921 tcaatgaaac aattgatact ctgcagatta tacgagactt agccataaag caagctaccg
    49981 gtcaggcttg gaccaaagat gacaaaacgg ctgccggtaa tttttttcag cggagcctat
    50041 tgaaagctgg ccgtaagacc atcagtgata aagctaatga ttccgagtcg tcacaacaaa
    50101 gattacgagc ccgtttagaa attagaaata tggccgggca gacgatgatt ttaattggtc
    50161 cggttatgat gccttaattt tatatattat gcctatcaaa aaaacagctc ctactaataa
    50221 tcctttacca gatcgtttgg gtgatggcga tatttcctta aaacaattat cattcggcct
    50281 gtggtatctg cgtcatcgcc gattatttta tagtttagct atcggccttt tagtggtgtt
    50341 ggtaattgct ggttggattt atgggttcta tggtttaggc cgctatttta tttttgatcg
    50401 tagtcgagat cgccagtcat tgctggacat agcgcaaagc ggtgtcaact tactggctgg
    50461 tcggactaat caaccgttga gtattagtga agttaagatt tttggcaata accctagcga
    50521 cctgctagcc gaaatttata acccgaataa tcggacagcc gtttatttta aatatgcttt
    50581 catagccaat ggacagccta ttgttcagcg tgaagggttt gtgttgcctg gtcagcgtaa
    50641 gtatttggtc gccaccggtc aacaggttcc cgccggagcc gcagtacaat tagctattac
    50701 gcaaactttt tggcaaaaac ttgatgcccg gcagattcct gattggccag cttttctagc
    50761 taatcatgat cagttggtta ttagcgatcg gcaaatcagt tttgccggct taggcgatca
    50821 agcagtaacg caggttaaat ttcgtctgga caacaaaact gcctatggtt attggtcaat
    50881 accaattttg atgttggtct accaaggcga taatttaatc ggcgttaatg aactggtttt
    50941 atctaaggtg aaatctggtg agtcgcggat ggttaatttt tcttggccgg gtcttagcgg
    51001 cgccgatcac ctagaagtcg tgacgatggt tgattacctt aataccgata actatctgcc
    51061 gttggaagat tagtttatgt tgggaaaact tcgccagtat tggcggcgca atgattggct
    51121 tttgacagta gcgatgctgc tattgttggc cgtcggtttg actgaaattt acggtgtttc
    51181 tttagggcgt ggcggcgtca gtttgttggc ttttcgcaag cagttaattt ttagcctaat
    51241 tggcttgatt ggttttatcg cgctatcttt atcggattat catcgttggc gcggctatgt
    51301 taattatttt tatggactag cgattgttct tttagtagcg gtgctagtct ttggtagtcc
    51361 tatcaatgga acacgtggtt ggtttaatat tcttggttta ggtttgcagc cagtagaatt
    51421 agttaaaatt attctaataa tttttttagc cagctatttc tctagtcgaa caatacgtat
    51481 tgatcaatgg aaaatttttt taaaaagcgg actagcgact ttggtgttaa tgattttggt
    51541 acttttacag cctgatttcg gttcggcttt gcttttaggg tctatttggt tgattttaat
    51601 cctagttatc ggttttgatc gtaaatattt atattggttg gctggtctgg ctattattgg
    51661 catagtcgtt ggttggcttt ttttatttac caatattcaa aagagtcgaa ttcgctcttt
    51721 ttttaacccc aatcaacaaa cctttagtca gagttataat gttaatcagg cgatgattgc
    51781 cgttggttcc ggtcgtattt tggggcgtgg cgtcggtttt ggttcgcaga cgcaattaaa
    51841 attcttaccc gaggcgcaaa ctgattttat ttttgccgtt attgctgaag agcggggatt
    51901 gttaggcgcc gggttagtgg ttttattgtt tgccgtgatt atttatcgtg ctctagccgt
    51961 tttaccaaag attaaagatg atttcggctg tttcgtggtg gttggcgcta tcggtttgtt
    52021 ggcgattgaa gtttttgtta atttggcaat gaatattggt cttttgccgg tggtcggcat
    52081 tgctttgccg ctagtcagct atggcggcag ctcgttgatt gccagtttgt tactattagg
    52141 tttagttaat agcgtcatta ttaggacgcg cagttaaggt aaatatatga atactaaaga
    52201 tttggataga ttaattgatc gtcaattaac cgataaatta gagaagttaa acttgcgcaa
    52261 tgattcaatt tatccgattc ccgatagtat tcaacatatt tggcaagttt ttattgatgg
    52321 tcagccagtt gagcattatg cgcatttagc tgctcaatcc aaatacggcg ttatgaattt
    52381 aggcattagc ccggatctgg gccagcatgt tacttttcaa gaaccaggcg gtggcggatc
    52441 agtaatcatc ccttttgtca ttattcaaaa acagcgagtt attgaattaa aaaggttaag
    52501 caaacagcag gattatcaag tttatgctgg cttaattgag caacaacgcg atcgtcaggg
    52561 tggtttagtt tggaatgtcc cgcgcggtta tgttgaacaa ggcgataacc atgatcagac
    52621 ggcagtcaaa gagttgactg aagaaaccgg cttagctggc cagccgtttc tattgcccgg
    52681 acagtcgctc aatcccaata gtagttattt tgaaaccggc aaggataatg gcgtgttaat
    52741 ttatgctatt aacttaggcc ctgatcgagc cgtctggcga gacagtcagg ttgttttatt
    52801 agataatcct cagccgacgg agatacatga gaagattggc caatgtcggt ttttcccatt
    52861 ggccgaatta tcgcaactag gcgatatgtt ctcagtggcg gctgaggcca gattaaggta
    52921 ttatcttgac aataagctat aaaattgtta atttccaatc atctttaata ggaggtctaa
    52981 aataatgaaa gacgctcaaa aactaacgcc gtcagcgttt gttgatttgg ctaaattatt
    53041 agccggtcaa ctgcgcttgc gttatgaaga attggatagt gaccatggtc gttcactaac
    53101 taattccatt aaccaatttg gtgaccaaac tctaattgcc gattgggagt tagaggaaag
    53161 tgtgatcaag ttttggcaat caactggttg ccaaattggt tggattgctg aagagcatcc
    53221 aatgctcttt aacaaaaacg aagttgattt cattggttat cttgatggtt tggatggttc
    53281 ctccgtctat aaaaccaacg aagatggccg ctgcggtaca atgatgtcga tttaccaggc
    53341 cgaggaccag acttttgctg gttatgtcgg cagtttagtt atggattatg gcagccataa
    53401 gtttggtggc aattgtagct atattgccgc caaaggcctg ggtgcctggg ctgatccagt
    53461 aactagaggg gactatatcc agataaacgt agtccctgat gatcaactaa cccgccaaat
    53521 gagaaggaag cttttggctt atgccgacct atattttgag tcggttcgcc gagctaccaa
    53581 gaaaatgacc ggaattaagt ttagatatct tcaggcttca atgccatatt atgtcgactt
    53641 agctcaaggt tatgcttgct tggtggttga atgcacgcgc aagcattctc tagagattgc
    53701 tagcgcttat ccgttagttc atgaggccgg cggagtgatc gtcgatctgg cctcgggcca
    53761 agatttggga gggcttgatc gatcgctagc agaagctagt gaacagatta ttgttagcgc
    53821 cccaaaccta aaaattgttg atgaaataac tagtcagatg agtgctagct attgacaaaa
    53881 aaatagtaat gtgctttatt gactagttag tcaagtattt ttaacaaaaa acctccacca
    53941 aataagaatg atgggtggag gaaaaatact caaagaagga gacaaaataa tgttagacaa
    54001 gttaaaagag ttggacttaa ttaccccatc aattcttccc atgcctaagg gcgtggtttg
    54061 gagttggcag ctctatatca acggtgagcc ggttgatatg agctttgtgc agcatatagc
    54121 aattaagtct cgtttcggca ccgtcaattt tggtgccgac gaaagggccg gctataattc
    54181 ggtggccttt gaggaggccg gccgtggcgg agcagtaata gtgccgtttt tctgcctgaa
    54241 agacggccaa gtcgtcgccc caaatcagct ctatgatgaa ctgtatattg gggttgtgga
    54301 acaataccgt aacaaacagg ggggcatggt cctcaatgtc cctcgcggat ttgtgaagca
    54361 aggcggtgaa ccacatgaag ctgcggctaa gcgcgaaata tttgaagaaa ccgggctgat
    54421 tgccgagccg tttctactgc ccggtcataa tctaaaccct aattctgctt tttttgaaac
    54481 tggggaggat ggcggatgca gaatttacgc cattgtctta agtcccgaac aggtagccat
    54541 cgatgacgat ggcattcatt taattaccga taatatccct gaagactcca gggaaattat
    54601 cggtaaatgt cgatttattc ctgctcacga agtctctcag ctcggcgaca tgtttagtgt
    54661 tgccgctgag agtcgtttaa taaactattt aatctcttta gacacaaact agttctaact
    54721 gagtttgtgt ctaaatgccg cttcactttg tgagcggcat ttttttaccc aaaattgaca
    54781 aattatcaat gattaactat actagctaat acgattaacc ctaagttaag tatttttttg
    54841 ttatgaaaag tgaattgaaa ttagccgcca gcactcgtca gcccggccgg caggccttga
    54901 gccagctgcg cagtcatggg tttattccgg cggtagtcta tggaccgaaa actgataatc
    54961 tccatattca aattaataaa gctgaagctc taaaaatcta caatcaggcc ggtggatcca
    55021 gcttgattac tcttgatatt gacggtcagg atcagcgcca agttattatt aaggatattg
    55081 cccagactag ggtcaaggac cagttgattc atatggattt ttttcaagta gatatggctg
    55141 agaaagttac cactgaaatt gaattgaatt ttattggtga ggcgccagct gttaaacaac
    55201 tgggcggcgt agtggttaag aattttgacg ctgtggaaat tgaatgttta cccaaagact
    55261 taaccaacaa gctggatgtt gatttatctg gtttagtcga ttttactacc cacattagtt
    55321 ttgccgattt gaaattaccc aagggcgtag tcttatcgga taagcacgag atgaatgaga
    55381 ttattgttgc tgtggaagag ccacgggcag tagctgtgga aaatgccgcc gctgaggaag
    55441 cagcaactac cgagaccaag accgagagca aaccggaagc cgaagaaaaa gcataattta
    55501 acaggttcta taattaaaaa acaacctagc agtgggttgt tttttaagtg cgccaccagc
    55561 cgaaacgctg tcggccggtt aaatcgggga aagattgttg ccaattgaga ccgccaaccg
    55621 ctaactgatt ttgaagttga gacacctgct gtaggtcgct ttcagttaac agatgaatag
    55681 tttgattggg atgatgctgg ataaataaag gtaattcctg agctaatttt ttaaaaagac
    55741 caaggccgtt atggctattt accagggcta atttcggttc atggcgtagt tcggtgtagt
    55801 gttcgtggta atttttcggt tttagatagg gcagattagc tagtaagaag atttgttgag
    55861 ccggtttaaa attagttgat agccaaggtt ttaataatga tccatgttgc caaatgattt
    55921 tagtattatg gagattggcg ttttttttgg ctattttgag agccgacgcg ctaatgtcgg
    55981 agccgccgta atagtcagca ttatttaatt cttgagccaa agtaataatc aaacagccgg
    56041 aaccggtgcc gatatcgagc caagcgctgg attcggttag ttttgaacgc aattctaaag
    56101 caatatcaac gagacattcg ctttccggac gaggaattaa gacagctggc gaaacaataa
    56161 attgccgact ataaaaatct tgccggccgg tgatataagc tagcggataa ccgttagtta
    56221 gtttaatcag cgcttgattg agctgccgcc attgtctggc agttaataat tggtcgggac
    56281 aagtgatcag gtaactagcc ggctgctgca gaataaaaga taatagccaa gagatgagtg
    56341 ctggctgagc ttgaccaagc ggccagcgtt gactcagcca atcctgtgct tggctaatag
    56401 ttggccgttt agcggtcggg ttcttgagcc agctgtttag catattgctg cagatgctgg
    56461 attatctcgt caatttcgcc agccatcact cggtcaattg agtgccaaga ttcttgaaga
    56521 cgatggtcgg taacacgatc ttgaggaaaa ttataagtgc ggattttttc actgcgatcg
    56581 ccgctgccga tttgttggcg gcgctggtta tcggccgctt cgcgctgttc gtcttctttt
    56641 ttctgtaaaa gacgcgagcg caatatttgc aaggcttttt ctttattttg atgttgggat
    56701 ttttgatctt ggcaggtgac aaccaatccg gtcggttcat ggataatgcg gatagctgaa
    56761 taggtggtgt taacgcattg accgcctgga ccagaagcac aataggtgtc aatacgcaaa
    56821 tcgctgggtt taatttcaat atcaacctct tcagcttctg gtaagacagc gacggtaata
    56881 gtcgaggtgt ggactcggcc ttgtttttcg gtttccggga cgcgttggac acgatgcgta
    56941 ccgctttcat attttagcca gccaaaaact tgttggccag aaatggcgca ggtgatttct
    57001 ttgtaaccgc cgataccgat cgggttggag ttgagaattt ctagtttaaa gccatgattt
    57061 tcggcgaaac ggctgtacat tctaaaaatt tccgccacga ataaggcggc ttcatcgcca
    57121 ccggccccgg cgcggatttc cataataaca tcttttttat cacgcggatc ggctgggtgc
    57181 agagcttcat ctaattgttg tgataaagca gcttgttcag cggttaattg agcttgttcc
    57241 aactcggcca tttggcggat ttcactgtca gtgctttcgg ttagtaattg gccagcttca
    57301 gctaaatgtt gttcaattga ttccaggcgg gttactaaag atactaaact ggctagttcg
    57361 gcctgttctt tagataatcg gcgaaatttt tgattatccg caataattgc tgggtcatta
    57421 agctggctgt tgagttcagc taatttagtt ttaagttctt ggtgcatatt cttcttaaaa
    57481 aattatctcc caaaccgagc ggctgggaga taatttatgg acaatgaatt tacagctaag
    57541 ccgccaattc tttttccaga gcctctttag cggcttgagc tttagcttcg cgagctttat
    57601 ttctagcctc acgtttagtt tttttactac gaatttcggt tttagattcc agcttggcgg
    57661 cgaatttttc aacgcggcgg gcagtgtcta tcaatttttg tttaccagtg taaaaggggt
    57721 ggcaagctga acagatttca gttttgattt ccgccatagt agaaccagtg gtaaagatat
    57781 tgccgcaggc gcaggtaact ttagcttcgg agtaataggt tgggtgtaag tcttttttca
    57841 tactataaac taaaaataaa cgcttagact agcgtgcttt tgttaagata tcatagcttc
    57901 agagaaaaat caagagccta tcgtcgcagt tgcaatttat tttaatttga cttatgattg
    57961 aataattggt aggacaagcc aaattttaac actaatttta atcttatgcc aaattttgaa
    58021 gcatttcctg acagcaacca tgttgaagaa aacgaacaga ataagcaaga acaagagcag
    58081 gcgcaagaac aagaacagac acaagaacaa gaacaagagc aggcgcaaga aaaaatgtta
    58141 cgctttatgc ttaatcgtcg gtatgatgaa agtggttatg agcatccatt tgatcagatt
    58201 cagaatgcta tgagagatta cccggcatta agtcaggaag acattgttcg catttatctt
    58261 gatgaacaag aatacgaaac ggttgctaat tttattgata aatttcccga ggttgaccat
    58321 agggccgtag ccgccaaatt agttgaagcc ggtaaaggta gccatgtctt gagaaatttg
    58381 gataaattta atggcgtaag tcgagaagat gtagaaagag atgattggca gcccgaagcc
    58441 gagtagtttt taaccgttag ggagcttgac agaccggtag ataatcggta agctagttag
    58501 tgatttttac tataaaataa gccgttaaca atttttaatt aacgactcat gcgttccatt
    58561 tttccttgcc cgatcggctt acgggtggcg gaacgcagcg gtttaaagga atttttatgt
    58621 cccaagtacc aactttggct gatttattag ccgccggcct ccattttggc cataaaacct
    58681 ccaaatggaa ccccaaaatg aaaccgtata tttacggcag taagcagggg ttgcatttga
    58741 ttgatttaaa taaaacccag gaacagatgg caaaagcctg gacatttatt accaaagaag
    58801 tcgccgccgg taaaaatatt ctactggttg gcaccaaaga tcaagtaaaa gaaccattac
    58861 gtcgggtagc ggagacaacc aatatgcctt atgttactga acattggttg ggcggtacgc
    58921 taactaattt tttcatcatc aagaattcta ttcgcaagta taaagatatt attgataaga
    58981 aaaaaaccgg tcagctagct aaatatacca aaaaagaaca actagaattt gatcgcgagg
    59041 cagctagact ggaagtcggt gtcggcggtt tagttaacct gattaaagtt ccggaaatta
    59101 tttttatctg ggacattaag aaagaaaaaa ccgctttagc cgaggctctt aaacgtcgca
    59161 taccgattat tgctatttgc gatagcaaca ccaatccaac cggtattgat tatccgattc
    59221 cagcgaatga tgacgctact aaaggcattg aattggtgtt gaattctcta gagcaggcga
    59281 ttatggctgg caaacagcag cgcgaagcgg ccaaagaaag tcatcagcca gttattaaag
    59341 agtaaattta aattagtctt tacattttaa ctcataactg tttagctagc tgcggcctgc
    59401 taaatagtcg acaattatat ggaaataacc atggaaataa ttaagaattt acgtgagcag
    59461 tccggggcgg gtatagttga ttgtaaaacg gctttaactg aggctggcgg tgatttgacg
    59521 gtggctatgg aaattttgcg caagaagggt attgccaaag ccggtaagcg ggccgagcgt
    59581 gaaactagtc agggcattat taaagcctgg gttagtccgg atcaacgacg cggccattta
    59641 attgaattgc gcgctgagac tgattttgtg gctcgcaatg aacagttcca ggggctggct
    59701 gacaatattt tacaggtgtt agttgatcat cagcctagcg atttagctgg tcttctaaat
    59761 ttaagccttg gcagccaaac ggttagagaa gcggttgata atctgagcgg agtgattggc
    59821 gaaaagatag tgcttagtaa ttgcgccact ttaactagtc agggaacagt ggctgcctat
    59881 ttacacgctg gcggttctat tggcgtctta gtggcgatta gtcaggctga atcaggtgat
    59941 ttggctaaag atatcgccat gcagattgcc gccagcaatc cgcgctattt aactcctgat
    60001 caagtgccga tgactgagat tgaaaaagag aaagaaattt accgtgaaca gcttagtcaa
    60061 gaaggcaaac cggcggaaat aattgaaaaa atattagccg gtaaaatcaa tcggtatttt
    60121 accgaagttt gtctgactaa acaggagttt attaaagatg aacaacagac tattgaacag
    60181 attctgggcg gagctgtcat tgaaaaatac tatcgcttcc aattataatt aaatagttta
    60241 agtttggtta tgaagctggc tttagtgcag atatatcctt gggatcggcc cagtttatgc
    60301 gagcctagtg aattaaccat agaaatcggc caacgcctgc tcgtagaaac cgatagtggt
    60361 caagaattag gccgtgtttt gggttttaaa tatctaagta atgatcagct ggcggcctgg
    60421 tcgggccata ccaaaaaaat tgttcggttg gccaattccg atgattgcca gacggttgat
    60481 cagtttgata aaacagcagc tcttagtcag tgccagcagt ttattgatca atgtcaacta
    60541 gaaatgaagt tgattgatgc tgccgtttcc gctgataacc gtcgccttac ctttgctttc
    60601 acggctgaag gacggattga ttttcgcgaa ctagtgcgtt tgctaaccag aaattttaat
    60661 aagcagattc gtttacaaca aattggcacc cgtgatgaag ccaaattatt tggcggtcaa
    60721 ggagtttgcg gtcgagggct ctgttgccgc ggatttttaa ctgaattatc gtcggttact
    60781 tcggacatgg ctgagattca aggtattaat catcgtggca gcgagcggat ttctggcatt
    60841 tgcggccgct tgatgtgttg tttagcttat gaagctgagg gttatcaggc tatggcccaa
    60901 aaattaccta atattggtga tcaatggaag gttaatggac agctaggcca ggtaattaat
    60961 cgccatattt taaaggggac tattatagta gaatttcatg atagtcaggc cggcggccaa
    61021 accgtagtgg agattactcc gggtcaaacc actaataaaa aagacgcgtc ggtgcaacgt
    61081 cgttggatcg ggcgtcatca taaataaatt tatgctgact attgatcaag ttaactgcat
    61141 tggctgtggt ctttgtgcca gtctttgccc agacaatttt aagattaatg acgatttcaa
    61201 ggctgaagtg gtggatggcg accagcttaa gtcgtgcgtt caagaagcga ttgataattg
    61261 tccagttcaa gctattaaaa aagcatagtt gcttcgataa taagaagatg atagaatagg
    61321 gctacggctc tattctattt ttattatgct aaccttagaa caagttaaac aattgaagat
    61381 acccagttgt tcgggctcgt atcaattttg tgttcaagat gggtcaatcg tttatgtcgg
    61441 caaagcgatt aacctacaaa aacggattta ttcttattgg cagcgttatc aagatttaac
    61501 gccagctaaa caacaaatga tagctgaggt tgtgactata aagtggctag aaaccgatag
    61561 cgaaattgag gcattactat tagaggccaa tttgattaaa aaatatcagc caaaatataa
    61621 tattgtttgg cgcgatgata agcgttacca atatattgcc atcagcgctg gtaattttcc
    61681 tcgtgtttat ttgactcgta aactagagga gtcgggtcaa ttttttggtc cgtttactag
    61741 taatcaggcc gctcgcgaga ctttacgaat tatccgtaaa atttggccct atcgttcttg
    61801 tcgtaattta ccgaccaaaa cctgtttgta ttatcggatt ggtcaatgtc cgggtccttg
    61861 ccagcagttg attagtgcgg ttgattatca aaagattatc caacagattg ttttattttt
    61921 gcagggcaag aaacagtcgg tcatcaagaa gattcaagtc gaattgagcg gttatcaaaa
    61981 gcagttagct aaattgaacc aaccgactaa gaagcaaaac gctagcgaag aagctgctaa
    62041 atttgaatac aaaattagtt cactcaattg gcaattacgc caactagagc aagtcttggc
    62101 cagtaaccac attttaggct tagaagaaaa atatgctaat gatgtagtcg aattagctaa
    62161 attattgggt tttactaagc cgccacaacg aattgaaggc tatgatttat ctaatctcta
    62221 taataaacag gcggttggtt cgctggtggt ttttattgat ggcgaggctt gccctagtga
    62281 gtataagcga tttaggttgt ctggagcgga aactgtcagt agtctgggcg acatcgcttt
    62341 attacgcgag atgttgacta ggcgattggc tcatcatgat tggccgcaac ctgatttaat
    62401 tgtcattgac ggagccaaag cccaagctaa tattgctcgg cgtttattgg atcgggctgg
    62461 ctggaatatt ccggttattg gattaggtaa agacaagggg ttaagatcgg cacgagcgat
    62521 ggataaatta ttttttcccg gtcagcagca agctttgcag ttatctttaa ataatccagc
    62581 gctacattta ttaaaaagag ttcgcgatga agctcatcgc tttgctataa cttatcatcg
    62641 gcaggttagg cgcaaaaaat tttgggagta aaattattta atctcctgtc taaactttaa
    62701 tgcattagct agcgttaagg gatcagcata ttccaggttg gcgccagtgg ataggccacg
    62761 agctagacgg ctgatttggc cgggccattt ctctaggagt ttattgatat agagagcggt
    62821 agtttcgccc tcaatggttg ggtcaaaagc taaaattatt tctttgattg ctggatgagc
    62881 ttgtacataa tcagccaaag cagccagtcg cagatcttct ggtttaattt cattgattgt
    62941 atccaaataa ccgcctaaaa caaaataacg gccctggtaa tcgccggtat tttcaatggc
    63001 ttccaaatct tgattggtgg ccacaacgca gagtaagtta tttaggcggt tggcgtcgtt
    63061 acaaataaag caggggtcgg ttagactaat agcgccgcaa agcgagcaag tggtcaaagc
    63121 ttggtttaat tgttgcaaat gatcagccag cggttcaatc agctctggtt tttgttctaa
    63181 aagataaaaa atataacgtt cagccgtttt agggccaacc gatggcaatt ggctaaatag
    63241 acggattaat tgttgaacca gcggcgggta agccataatc tatagtccga ggttggacaa
    63301 tccacccatt tcttgcattt ttttggccat gacgcgttgg gtttttttaa cagcatcgtt
    63361 aaaaacatca cggcagatgt tagctaattg ttctttaggt aagtcttgct tgataacgag
    63421 tgagagcact tccatattgc cgttcatttc aatagtaacg ccttgctttt cagctgtaac
    63481 cttttcgcca gctaaggcat tttgcatggt tttagcttgg ttgcgcagat ctttaaattg
    63541 cttgagttta ttaaacataa tctttaatta gccggcggtt atgccgcttt tatctaaatt
    63601 tttaaaatta accgtatttt catcaaaata taaatcaact ttaccggtcg gaccgttacg
    63661 gtgtttagca atgtgaattt cagcgacatg tttttcaaaa tcgttcaggt tttcagtatc
    63721 ataattacga tcagccgcct tgcggtagat aaacatgacg atgtcagcgt cttgctcaat
    63781 agaaccggat tcccgcaggt gagccagctt gggaatagcc ggtttagttt gttcaacggc
    63841 tcgcgataat tgcgataagg ctactactgg aacattaatt tcacgagcca ggcctttgag
    63901 tgatcgggtg atttcagcaa tttcttgaac gcgattatca ttaatccggc cgcgaccttc
    63961 cattaattgt aaatagtcga gaataattaa atctaagccg tgttcagatt gcagacgccg
    64021 acatttagtc cggatttcca taatattaca attgggcgag tcgtcaatat aaattgaaga
    64081 ttcagctaag gcgcccatag cgtagctgat gcgaccgaag tcatcatcgt cttcgcggtc
    64141 ggacaattgg ccagtgcgca ttttccataa actgatatta gcttcagcgc aaagcagacg
    64201 atcgactaac tgctctttgc tcatttccag cgagaagatg ccaaccttgg cacggctttt
    64261 aatagcggcc tggcgggcta aatctagagc aaaagaggtt ttaccgacac tggggcgagc
    64321 ggccaaaata attaaatcgg atttttgtag accagataat aatctatcta agtcgacaaa
    64381 gccggttggc aagccgcgca aacggccgct ttgtttatgc aattcatcaa ttcggtcaaa
    64441 agcgtcagtt agtaggccgc taattggctg gaaagattgt ttgagatatt gatgagaaac
    64501 gccaaatagt ttttgttcag cctgatcaag gacttgagct aaatcatcgt cttcccggta
    64561 accaagttca ccaatttctg aggcggcagt gataaggcgg cgcaaggttg atttatgctt
    64621 gaccaattca gcataactga cgacattagc ggcggtagct acgccttgca ggaggctggt
    64681 caagtaagaa cgaccgccaa ctgtatccaa ttgattttta gctttaagct tatcggttaa
    64741 gttcaccaag tcaatcggtt cgtggttttg gtagagagcc aacatggcgg cgaaaatcag
    64801 accgttgata tcgttataga aatcttccgg tttaactata tcggcgatct taacaatagc
    64861 cgccttatca atcaataaac agccaagcag cgatatctca gcttcttggt tttgcggcgg
    64921 cattttgata gtgttatttt tgctagccat agggggtaat ttagaccgct gttggcggtg
    64981 gtaaagcctt tttatcatac acgctgtcca gctctttgtc cagtcagttt taccttgtcc
    65041 acgactgctg gccgtgatat aatagcgaat gaatcaatta atttttatag tgtagttatg
    65101 ccgcacaggt tttgtccatt gctagttgtc gggttactat tggtggctgt tccagccctg
    65161 gctaatggtt tgcctgatac tgatggcgac ggagttcccg atcaagatga aatcaatatt
    65221 taccaaacca gcgttgatca ggccgacact gacggcgatg gctatagtga tcgtcaagaa
    65281 ttattggccg gctattcgcc gcataacccg aaaaatatta aattaattga taacgatcag
    65341 gatcaagacg gcttaagcga tttttgggaa ttgcgtttca gcactaattt attaatggcc
    65401 gatactgacg gcgataatat caatgatggt gatgagattg accaagcgac tgatccacgg
    65461 caagtcggcg cggttaaact accaattaga ttggatgttt ccctaaaacg acaggagttg
    65521 acttattggc tggctggcgt taagtttaag cagtttccgg tttccagcgg ggcgccacgg
    65581 acgccgactc cgattggtca ataccgggtt attaacaaat taccgaaagc ctggtcgcct
    65641 tatggtttgt ggatgcctta ttggcttggc ttaggtcgcg gacgatttgg ctttcatgaa
    65701 ttaccaattt ggcctaatgg ttatcgtgag ggtgctaatc atttaggaaa agctgtctca
    65761 catggctgta ttcgcttggg aattggtccg gctcaatatc tttatgatcg ggttgatgtg
    65821 gggacggtgg ttaatattaa tcggtaatta ttaagtaaag aattatttat gttatatagt
    65881 cctcaagtta tccaacactt tcaaaagccg cacaatcaag gcagcttggc tcatcccagc
    65941 gccgtcggtc aagttggcaa tccggtctgc ggggatatta tgaagattta tttacggatt
    66001 aagaatggcg ttattaccaa ggttaaattt gagaccttgg gttgcgccgc cgccatagct
    66061 gttgcttcgg ctttaaccga tctggttaaa ggcaagacac ttgacgaggc ctatcagatt
    66121 actaaagatc agattattga tgacttgggc ggcttaccgg caccgaaagt acattgttca
    66181 atgcttggcg ttgatgctct gcatctagcg attgataatt atcgccagca attagttaaa
    66241 taatttatgg ttatggataa gattaaaaat ttggcctggt ggccagaatt agagaaaatt
    66301 attgatcgcg aaattcgtcc cagtctgcaa atggatggcg gcgatatccg tctggttgat
    66361 tttgatgagg ccaagggaat tttgtcggtt gaattgcaag gccattgcgc tcattgcccg
    66421 atgatggaat atactttaaa ggaaggtatt gaagctgagc taacagctcg actgcccaaa
    66481 attaaaaagg tacaagccgt ttaattatga aggtttattt agattatagt gctaccaccc
    66541 cagttgatcc gttggtttat aaggcaatgg agccttattt ttctcaagtt tttggcaatc
    66601 cagcttcatt acatcattgt ggtcaggcgg ctcaggcggc agttagccaa gcccgccaaa
    66661 ctattgctga tttttttggc gctcaagcta aagaaattgt ctttacttca ggagcgaccg
    66721 aagctgataa tttggccgtt aagggtttga ttcagggatt gttaaaacaa aagcctgatt
    66781 tgcggccaca cattattgtt tctagtgttg agcacgaagc tgtccgccag ccatgtcagc
    66841 agctagccaa agaagggatt gaaattagct ggttgccggt tgataaaaaa ggtctagttg
    66901 atttggttaa attagaaaaa ttgattaaaa ccaatacggt actagtgtca gtaatttatg
    66961 ttaatagtga gaccggcgcc cgacagccga ttcgagaaat tggccaaatg attaaaaaaa
    67021 ttaatcatcg ccgccaagaa gattggcgca aacgcagcgg tttagccaat aagcggccgc
    67081 aaaccgttta ttttcatacc gatgccaccc aagcgattaa ttattttgat tgtcgggcta
    67141 attatttgca ctgcgatctg ttgagttttt ctggtcataa aatttatggc cccaaaggta
    67201 ttggcgggct atatgtgcgg agtggtacac cattggcggc tattcagctc ggcggtcatc
    67261 aagagctaaa tcggcgcagc ggcactttga atgtcccggg tatagttggt ttagccaaag
    67321 ctttagagct agttaatcgc catcgcgccg ctgataatcg aaaaatagcg gccttgcgtc
    67381 gccggttggt tcaagggcta gtcaagaata ttcccgatat cattttaaat actgatttaa
    67441 gcaatgcgag tccaagtcat gcccactttt cagtgctttt ggctgaaggc gaggccttgc
    67501 ttttggctct ggatttagcc ggcattgccg tatccactgg atcagcgtgc gcggccggta
    67561 gcctagaagc atcggccact ttattggcta tgggtattag tcaagaagtg gctcattata
    67621 gtcttagatt cacgttggga cgattgacta cggttactga gattaattat gttattaaaa
    67681 aattaccagc catcgttgct cgtctgcggc gctgggctcc agatttaaat aattaatatg
    67741 cgcttattat tgtctaagtt gcttaattat ttctatttat tcggtcagaa ttggcgaata
    67801 acttggtcag atatttggca tgattccggt catcgctggt taactattat tgatattatt
    67861 ttattagtta ttagttggat tttagctttt tggctggcgg cagttggtcg tggcagtttg
    67921 ttaattctgc actataatat taattttggt attgatttaa ttggttcagc caattctatt
    67981 tactggttgc catttggagc gactgtggcg gtcgtcgtca atagtttgct tttacagatt
    68041 aattatccgg cagccaaaca attgcgtttg accagtttag ccggtagctt ggcagttttg
    68101 attttgatta atttagcttt agccagtttg cttctaatta atttttgtta agtgctatat
    68161 gtttgatttt tacattatcc gtaccctgtt attagctgcc ggagctttta tttttactat
    68221 agcctggacg ccgttgctga ctaatttttt ataccgccat aagttgggta agaaaattcg
    68281 taatagcggc gcaacgccaa ttttttcgca actgcacgcc gctaagagcg gtacgccgac
    68341 catgggcgga ctgttaattt gggtaaccac cttagtgatg gcgcttggat tatttttggt
    68401 taataaatta tggcctggat catggctagg gcaactcagc tttttaagcc gcagccaaac
    68461 ttatttgccg ctaggaatat tggttgcttc agcactgatt ggcttgtttg atgattggtt
    68521 ggatgttcgt ggccgtggca ctaaaggtgg tggcgggcta agcttgcgtt ccagactgct
    68581 tgtttatgcg atcattgctt tgattggcgc cctctggttt tattttaagt tagattggga
    68641 catttttcat ataccctttc tgggtaattt ttctttaggt tggtggtata ttccggtttt
    68701 tatttttgtc attatcggga cggctttttc agttaacgaa actgatggtt tagatggact
    68761 agccggcgga acgctgctgt ctagttttat gatttacggc gccattactt ttttaattgg
    68821 ccgctatgat ttaaccgctt tatgtgcggt tattagcggc gcgttattgg cctttttatg
    68881 gttcaatatt aacccggctc gtttttatat gggcgatact ggttcaatga gccttggtat
    68941 tactttaggg gtgattgcca tgctaacgaa tacggcgtta attttaccag ttatcggctt
    69001 ggtatttgtt gttgaaactc tgtcggtaat tattcaggta ttgtccaaaa aattgcgtca
    69061 tggccgcaaa gtttttttat cagcgccgat tcatcatcat tttgaagcta aaggatggac
    69121 tgagcctaag attgttatgc gtttttgggt aattgctgcg gttagtggag cgcttggtgt
    69181 ttgtatattc ttacttgatc atactattta atttacatga aatcggtagt tattaagaaa
    69241 aaaaccacaa ttgccgaaaa gcggtcgact gctcaaccga tagctagagt tacccgtcag
    69301 ccgttagatc gttttttatt aattactgtt gttttattgt gcgctattgg tctgttttgt
    69361 ttagctagct cttcgtcggt agtagcttat acccgttatg gtgataccta tttttggtta
    69421 aagaaacagg tgctgtcaat gctaatcggt gccgctggat tttatctgtt cagtttgatt
    69481 gattatcgga aatggcagaa gctgggcggc ttaatgctca ttattgcggt cggtttttta
    69541 ttaactcttt ttacgccgtt agctcatgaa caagctggca cgacagcctg gctgaatatt
    69601 tttggttttt ctttacagcc atcggaattt gttaagttat tcctaatcct ttatttatcg
    69661 gctttgatgg ctaaattagg gtctagtgtt aatcccgagg agaagcagcg tcttaattgg
    69721 tttttttggc ttgttttggt gataatcggt gggctgattc tagctcagcc tgacttaggc
    69781 acattattaa ttatggtggc taccagtata gtgatttatt tattgggtgg tggcagttgg
    69841 aaattggttg gtcaactaac ggctgtt
//
LOCUS       DAOPAI010000003        54020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_709_length_54020_cov_5.770536, whole genome shotgun sequence.
ACCESSION   DAOPAI010000003 DAOPAI010000000
VERSION     DAOPAI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 54020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54020
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_54020_cov_5.770536"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKV49_00820"
     CDS             complement(<1..322)
                     /locus_tag="PKV49_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22195.1"
                     /translation="MKRQRLINKASSPADWPIEKRRLLAWVGITAIMTILLVFWFYSL
                     RLSFISKQKNQEQPLLDINQIRQDLDAGLEEVNKGSELLEKIATTTDSSTTPEARLLQ
                     EQNRL"
     gene            complement(327..983)
                     /locus_tag="PKV49_00825"
     CDS             complement(327..983)
                     /locus_tag="PKV49_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL22196.1"
                     /translation="MIISWLGQSCFKLQDKMNGRDITVVLDPFSPSHTGLKLPANLSA
                     DVVTISHQHKDHNFLSGVSGQPFVIDMAGEYEIGGLFIDGVDSDHDQQGGAKRGHNLI
                     FRLGFSDLVVTHLGDLGSTLEAKQLERLAGTDILLVPVGGYYTLDAKQAIEVINQIEP
                     KIVIPMHYKLPGLSFNLAAVDDFVKAIGLAPIYEDKLKVVRRDLDSENLQLIILSVTA
                     "
     gene            complement(1097..4018)
                     /locus_tag="PKV49_00830"
     CDS             complement(1097..4018)
                     /locus_tag="PKV49_00830"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPL22197.1"
                     /translation="MAQPFDYLAGLNPAQRQAVEHGSGPLLIVAGAGTGKTTVLINRL
                     LHLIMSGQAKPDQILLTTFTDKAAGEMEERADRLLPYGYTDLWIQTFHGLGERLLRQH
                     GLDIGLPTNFKLLNETGQWILLRKNLDRLQLDYYRPLGNETKFLRDLVKYFSHLKDEN
                     ILPADYLQYVDGLDLSDAGQPEQLEVARLQELARAYQTYNQLLLDNSCLDFGDLISYT
                     LRLFQQRPNILQQYRQQFKFILVDEFQDTNWAQYELIKLLAAPDNNLTVVGDDDQSIY
                     KFRGASLANILQFKDDYPAAVNIVLTDNYRSGQPILDQAYQSIQKNNPDRLEDRLKIN
                     KRLVAVGQPNGSVEVWPLASESEEIVAIVDYIKEEYRQDPAAKWSDFAILIRSNRDAD
                     KFINELGRQKIPCQFMSLRGLYYKPIVLDCLAYFKLLDNYHESTALYRVLNSRPFALS
                     PADLVELSALARRKAWSLFEALQNNALAVKISPDGRRAIDKLLKLVSAQSLSAVQQSP
                     SRLFVDFVYQSGLLAGLDQEKDSAIFHYLNQLYKKMKEFEDSGPDLHLKDFVDLMNME
                     MEAGETGSLSLPLEDDDVVKIMTIHSAKGLEFRYVFLPDLVDKRFPTTKRGDSLPVPD
                     GLIAEPVGSGDVHLAEERRLFYVAVTRAKEKLILAYARDYGGVRDKRPSIFLSEIGLT
                     ANQTERQLDADRVFELVREVEALKNSDNNQILPAPLVAVKKFSFSQFESYSNCPLQYK
                     FAFVLRVPVPDKGNIVYGRLMHRCLYELFAPCLLNEQPTDLFGQSTVPTKPDWAGLMA
                     IYKKNWVDDGYDSAEDREKYRLLGQQSLKLIWQQIEAGGWPNVLALEKKFVWKTAQFT
                     LGGTIDRVDQLADGTVEIIDYKTGKPKDKLDYQTKRQLMIYQLAVESVFGWRVGKLTF
                     YYLNSGQALSFTAKPADLDKVQQEVAAQVEAIRQGFFPAKPSQLCAYCDFRSICQFRQ
                     V"
     gene            complement(4030..5319)
                     /locus_tag="PKV49_00835"
     CDS             complement(4030..5319)
                     /locus_tag="PKV49_00835"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPL22198.1"
                     /translation="MWQRQISNERPLILKIPLAGCPTFTALVVIAVGSKYETANQSGL
                     SHFLEHMFFKGTSKRPSTLAISSELDAIGGEYNAFTSKEYTGYYVKCAADQAAVALDV
                     LSDMLLNSQFSSEEIDRERGVIIEEINMYQDNPMLHLEDVFEQTLYGDTPAGWDTAGR
                     KETVSAFQRDDFINYWRQNYGQQRTIICLAGQLPENIDSLVNKYFAQWPAAINFQDKL
                     PVVEQQSQPQLLVADKDTDQLHLALGVRAFPYGHPQRLPSRLLAAILGGSMSSRLFLQ
                     IRERQGLAYYVRTSVENYTDSGYLVTSAGIKKEAVAQAVATIIAEYKKLTTELVDAAE
                     LERIKKFLSGRLILGLEGSDDLAQWYARQQVLFLGQGNVQENLDTPTEYLAKIRAVTA
                     EQIQSVARQIFVDQGLNLAIIGRVTEPDKLLAHLSFN"
     gene            complement(5325..6710)
                     /locus_tag="PKV49_00840"
     CDS             complement(5325..6710)
                     /locus_tag="PKV49_00840"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPL22199.1"
                     /translation="MSEVTLESLVSFCKRRGFVYPSSEIYGGLAAVYDYGPYGVELIN
                     NIKQIWWRELVYRYDNISGLDSAIFMNPRVWQASGHVAGFSDPLTECRYCHTRLRVDH
                     LLEGVGAEASDTMSETEINQLFDDYRHLVVCPECGHRDFTQVRHFNLLVHSNLGNFTG
                     NDSNPVYLRGETCQGIYVNYKNILDSTRLRLPFGIAQIGKAFRNEITARQFIFRTREF
                     EQMELQYFVRPTNDLVDFNLWKQRRWDYYINILGIDPANLRWHEHEKLAFYAKAACDI
                     EYNFPFGFQEIEGIHARGDYDLTQHQKFSGQELDYYDQAVDQKFIPHIIESSSGVGRV
                     FLMLLNEAYHEEELGENDKRVVLKLKPALAPVKVAVFPLLRNKPELIKAAQEVFDQVK
                     MRWRCEFDDNGNVGKRYRRQDEIGTPYCLTVDFDTLEKGTVTVRDRDTMKQTTVQLNE
                     LVSYLSSKLDY"
     gene            6876..7184
                     /locus_tag="PKV49_00845"
     CDS             6876..7184
                     /locus_tag="PKV49_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22200.1"
                     /translation="MSFEEKVIVSGAIIWSKSFHFVVKNCDGQEIMLKAGAIEASAKS
                     RLQVARIGCAECQFITEILKKRPIELSREDITNLNFSAILEVLPPMKKKRKKEPKKRR
                     "
     gene            complement(7292..7966)
                     /locus_tag="PKV49_00850"
     CDS             complement(7292..7966)
                     /locus_tag="PKV49_00850"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HPL22201.1"
                     /translation="MSICQIPAQLPDAASWLKFDLAKFSNSELLAFIISQGQDIKACI
                     PLAKIILKRIPAPCLVRASFEELVTAGLNSQQANLVLASTGMTRRFCRQRVQNIYYSQ
                     EVIYQEFKYLRSCTQERLAIGYLDRRCHELRRKNFLFEQLKQPYFHPQQIFEPILQLP
                     AQQLITASNHLVGGAEPNELDLLLFNKLIIMCQAFGLQLLDNVIFSRRGFYSFRQAGL
                     LKPSAS"
     gene            complement(8123..10390)
                     /locus_tag="PKV49_00855"
     CDS             complement(8123..10390)
                     /locus_tag="PKV49_00855"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPL22202.1"
                     /translation="MQKQEIVVSRDWHGRPLTIKTGRLAHQADAAVTIQYGDTVVMAT
                     VVEAKEAREDGDFFPLMVDFEERLYAAGIIKGSRWVKREGRPTDEAILSGRMIDRSIR
                     PLFDESSRRDVQVILTVLSVDQTNDYDIVALVAASAALRISGINWRGPIGGIRVGRID
                     NQLVFNPTYKQREISDLDLIVAGTASKVIMIEAGAQEIPEAAMFEAIMAGQQALAGSI
                     ELIEELYQAIGPIEPKKAKTVRLSADELQAQERKQQAQKIAEQWLDEHIEPILFDKIY
                     YTKGQRKLAVEAIKDGLDEYLFSQNFDRSLRQWVIGQTVEVAVEAAVTNNILQSDRRV
                     DGRALDEIRQLSADVSILPRNHGAGLFSRGETQVMSIVTLGAPSDEQSLEGLEGVSTK
                     SYMHHYNFAPYCTGEAKPIRTAGRREVGHGALAEKALVPVLPSKADFPYTIRVVSETL
                     SSNGSSSMGSTCASSLSLMDAGVPIKRPVAGLAMGLASNQDMSQWKVLTDIQDLEDGQ
                     GGMDFKITGTTEGITAIQLDTKTDGLTKEIIQTTIEQSRRGRLQILEVMNQAISEPRA
                     ELSPYAPKIVSFFIDVDKIREVIGAGGKVINEIIAACEVTIDIEQNGQVMVCGSNQEK
                     LNQAVKWVQDIARKLEVGEKFTGKVVRLLDFGAFVELLPNRDGMVHASELAPFYVGKP
                     ADMLHEGDTVTVKIKAIDDQGRVNLTMKDLSDNQAIWERYKAKGLPTERPTGFPGPRH
                     FSSRSGDNSSRPHLG"
     gene            complement(10499..10999)
                     /locus_tag="PKV49_00860"
     CDS             complement(10499..10999)
                     /locus_tag="PKV49_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPL22203.1"
                     /translation="MVKHPEQRVGVLVDVANLYHSARNLYNKKVNFRKVLAAVVADRK
                     LIRAAAYVVRSDNNDDETAFFEALSQQGFEVVMKDLQVFAGGAKKGDWDVGISIDAIK
                     LSHKLDVIVLASGDGDYTPLVNYIQSTTGCLVEIAGFRQTTSAKLLEIADDFINLSDR
                     QFLISR"
     gene            complement(11133..11393)
                     /gene="rpsO"
                     /locus_tag="PKV49_00865"
                     /pseudo
     CDS             complement(11133..11393)
                     /gene="rpsO"
                     /locus_tag="PKV49_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
     gene            11535..12479
                     /locus_tag="PKV49_00870"
     CDS             11535..12479
                     /locus_tag="PKV49_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPL22204.1"
                     /translation="MLKDDINHFLEYLEVERGLSNNTLIGYRFYLQRFLAFATASGIN
                     KPNQISQELIHRYRLHLNRLANREHDNLKKNTQNYHLIALRQLLKYFSKHNIESLDSN
                     KVELAKQEPRQVSFLEGYDLTAFLQAPLTIKNSSKLVRLRDRAILELLFSTGLRVSEL
                     ARLKINDINLKKAEFTVRGKGNKLRLVFLSDDAKAAIKSYLEARQDTEPFLFISHGKI
                     NQETNPVYGLTPRSIERLVGKYALAAGITKKVTPHTLRHSFATDLLMGGADIRSVQQL
                     LGHASITTTQVYTHVTDQQLRQVYQNFHGKSRKNLRQS"
     gene            12546..13124
                     /gene="clpP"
                     /locus_tag="PKV49_00875"
     CDS             12546..13124
                     /gene="clpP"
                     /locus_tag="PKV49_00875"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPL22205.1"
                     /translation="MPLIPTVIETTGQIERAYDIYSRLLKDRIIFLGEPIDDHTANLI
                     IAQLLFLDAENSSKDIKFYINTPGGSVTAGLAIYDTMQFIKADVSTICVGMAASMGAV
                     LLAAGAPGKRLSLPNSEIMIHQVMGGFEGQATDIKIRSEHILHIKDRLNKILAQHTKQ
                     DLKTIERDTERDHYLTPTEAKNYHLIDKIISR"
     gene            13341..14855
                     /gene="rny"
                     /locus_tag="PKV49_00880"
     CDS             13341..14855
                     /gene="rny"
                     /locus_tag="PKV49_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPL22206.1"
                     /translation="MNYFIGLIALAIGGSFGYWLRRQVAINKATSAENKAEKIIDEAK
                     VKEKEILLKAQDKALKLIDEAKQENDSHRKEITAWQNRLEKRETAFSQKIMELQDKQQ
                     QLYDKINQVETTKEEINQIKSEQTAKLEEIAGLNIEQAKELLLEKIKTEASDSLMSRL
                     DKLNNENSEQIEEKSRELMAAAMQRLASTFTAEVTTTVVDLPNDEMKGRIIGREGRNI
                     KTIEQLTGVEIIVDDTPNAITISGFSGIRRQIAKRALDYLIKDGRIHPTKIEDAIEHA
                     KQDLALDIKKAGEDALYELGITGFDSKLIQIIGRLKYRTSYGQNALRHSVEVAHLSGL
                     LAEELGADATLAKKAGLLHDIGKAVDHEVKGTHPQIGGNIARKFSLSQEIIDPIETHH
                     DDHPKGLISVIVKVADAISSSRPGARNDSLDQYIQRLEELEKIANSFDGVDKTYAIQA
                     GREVRVFVRPEEITDLDAHELSVKIAKQIEQELKYPGEIKINVIRELRIIEYAR"
     gene            14864..16759
                     /locus_tag="PKV49_00885"
     CDS             14864..16759
                     /locus_tag="PKV49_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPL22207.1"
                     /translation="MSIGSIIRKIKKWRPVTKRQPVHHILDQQIVFRLSKGRLPGWRQ
                     WRYLPRFLSHKEKIIIIACLVTLLASLTIITNHYYRHHRQVIPKKGGYYVEGLIGTPR
                     YINPLYSSMNQTDRDLSRLIYSSLLTRDQQGKLVNDLAENYEISPDGLIYTIKLRDNV
                     QWSNGSRLTADDVVFTYQAIMDSNYNSPIKNSLGGLDVTRVDDLTIRFTLSQPYIAFL
                     DLLTIGILPAEIWSQIPAESASLADLNLKPIGSGPYQFKSLTKDQLGTIKTYTLTIND
                     KYFKDQPYIKELTFKFYNNYEELVAALNDGSINGANYLPQTAAKKLLAANRYNLRYLG
                     TPQLVAIFINQDDQALKNKALRQALALAIDKNQILLENDTLVSHLANGPILKQSQYYD
                     ANLTRPNFDVNKANQLLTDDGWQIKTITADEVAAAEKGLADNQGDQDQQKTITDLGVG
                     QWRQKNNQYLTINLTTINEPDYLLIANQLALTWQKIGIKISLNIVDTDNINSQIIRQK
                     NYSLLLYGQNLGSENDLTALWHSGQSGANGFNLSNYRNEKVDKSLETMRSSLELAARQ
                     QAAKELQNQIVNDLPAIFLYNASHLYIQHKKINGFDTDFLVEPADRFNEINHWYIKTS
                     YRWKNKN"
     gene            16764..16847
                     /locus_tag="PKV49_00890"
     tRNA            16764..16847
                     /locus_tag="PKV49_00890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16798..16800,aa:Leu,seq:cag)
     gene            16908..18731
                     /locus_tag="PKV49_00895"
     CDS             16908..18731
                     /locus_tag="PKV49_00895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPL22208.1"
                     /translation="MITKYRTKDSAGQAASGSYGASRRPNQRTNYHRSNTLSQNQGLD
                     KLRVIVLGGLEEVGRNMTILEYGREIIIIDMGLQFPEEDMLGIDYIIPNTDYLTGKED
                     WIKGVVITHGHYDHIGAIAHLMGKLKNPPMFMGKLTAGLVEKKVKEHPQCPRLKITRI
                     EEDSKIQLGKNFRLEFLRVNHSIPDCFAAIVHTPIGSVMHTGDFKIDYSPVNDKPADL
                     QRIAQVGSQGLLLLMADSTDSTHPGYQVSESTIGDEIGRLFEKIDGRIIIGTFASQVN
                     RIQKIFSLAEKFGRKIYLQGRSMNDNVEIAKQIGYMKFDAKTIINDSELRHIPDNQVI
                     ILGTGAQGENGAFLMKVVNNEHRVVKIKPGDTIIFSSSVIPGNERSIQSLKDQIVRQG
                     GKVIHYEMMDVHAGGHAKQEDLKLMMRLLKPKYFMPIEGNHYMLQAHAELAQQVGIPS
                     NNIFVADNGQVVTLQRQGHEIVGQLTKEKVNTDYVMVDGLGVGDVSDIVLRDRKTMAE
                     DGMIVVIATIDSKTGDPIGNPDIISRGFIYMKDNKDLIQDTRMRIKKIVKETDFLANP
                     ISGHGFNDDDQLKNKIRADVSQFLFNKTKRRPMVLPVVIKV"
     gene            complement(18862..19563)
                     /locus_tag="PKV49_00900"
     CDS             complement(18862..19563)
                     /locus_tag="PKV49_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22209.1"
                     /translation="MTYKLKQGIIIQNDKFLDSKLSLAAVIFHETMHLKAFTALQSDQ
                     GVKDFSLFRSGVSAHSRTGHEMPKHVHFKGLHEAIVSEQEIRTLDKIMLLPNFREEHL
                     FLEIPIVKEQIANFKRQNNINSDSFVWFNIQQNCLEVVETYNQHRKLLKYICQEIQQE
                     FSDQYADADTIFTEFLRAHFTGSLLTIGKLVEKTFGPGSFRSLGNVSEDENSVNLCFE
                     DLSRRRKQLAAIDHQ"
     gene            complement(19746..19868)
                     /locus_tag="PKV49_00905"
     CDS             complement(19746..19868)
                     /locus_tag="PKV49_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22210.1"
                     /translation="MERIPNLQVIGQADPKDKEQTKDFLVPGFLSIMPHYRIKI"
     gene            complement(19872..20201)
                     /locus_tag="PKV49_00910"
     CDS             complement(19872..20201)
                     /locus_tag="PKV49_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011130058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HPL22211.1"
                     /translation="MEFKEYQQQSHKTAVYPVIGQGFVYPALGLAGESGEVVEKIKKI
                     FRDQQGIVGDEQRQAVAKELGDVLWYLAQLATELDLDLNQVAQDNLDKLFSRQQRGQL
                     HGSGDER"
     gene            complement(20206..20691)
                     /locus_tag="PKV49_00915"
     CDS             complement(20206..20691)
                     /locus_tag="PKV49_00915"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPL22212.1"
                     /translation="MGIFQEILSSKELWTIVYSAIPVTELRATIPLAITVWHVKPLTA
                     FILAVIGNSLPILVIILGLNKIVQLAERYSKFWTRVIEAVFRRTRRKTSQQFAQYGLL
                     ALFLFVAVPLPMTGAWTGSIAAWLFGVPARRAFWPIFAGVIVAGIIVTSITVGTAAIL
                     K"
     gene            complement(20748..21164)
                     /locus_tag="PKV49_00920"
     CDS             complement(20748..21164)
                     /locus_tag="PKV49_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22213.1"
                     /translation="MKIIICGSMSASKEMVEAEKQLLTLGHEVVLPEFTHDYAAMATI
                     DKAHTESARNKVQYDLIRIHFDKIKAGDAILVVNVERKDIPGYIGGNSFLEMGFAFVL
                     NKPIYLLYQIPQCNYRDEIEAMRPIVLAGDIGKIGL"
     gene            complement(21264..23444)
                     /locus_tag="PKV49_00925"
     CDS             complement(21264..23444)
                     /locus_tag="PKV49_00925"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPL22214.1"
                     /translation="MVPTIFWFIPGTGLLVLLVARFFFVEMKKKHPGDNRMQEIAGLI
                     RQGSMAYLWQQNKFVAVAFLVISILLSVAAFGFNLMSKIVPFAFITGGFFSALSGYIG
                     MNTSTLASSRVAQAAKTSLDAALQIALRSGAVMSMVVVGLALIDISGWFCFLNYFYTG
                     DSASRLIVITTTMLFFGMGASTQALFARVGGGIYTKAADMGADLVGKNEFNLTEDDPR
                     NPGVIADNVGDNVGDVAGMGADLYESYAGGILAAAAIFASASHGLSPELQVRYVLFPM
                     IIGAIGAICSVISIFTVKAKNNSGMKELVGSINKGIWLSSILIAVATFVVAWQLGLHN
                     WLGLSLAVVSGLLVGIVIGVWVNYQTSQGYRPTQRIAENAETSSATVVTGGLALGMKS
                     TSVPVAAVCLGTIGAFYFANGFSLDIVNISQGLSGIAMAAVGMLSIIGFVLATDVFGA
                     IADNAGGNAEMAKLPKEVRERTDQLDAVGNTTAAIGKGFAIGSAALTAMSLLAAYLEE
                     IKIALLRSGDFVIVGGQKLFLAQANIVDLVNYYEIHVMNPKFIVGAFIGAMVVLVFVG
                     LTTEAVGRVAKKMVAEIRRQFSNPRILDGSENPDHKACVRIATRGAQKEMIGPALLAI
                     VVPIMIGILLGVAGVMGLLLSAMTTGFGMAVFLANAGGAWDNAKKYVEEGHFGGKGSD
                     AHKAAVIGDMVGDPCKDTSGPALNILIKLMSMVAIVTVGITVLF"
     gene            complement(23551..23907)
                     /locus_tag="PKV49_00930"
     CDS             complement(23551..23907)
                     /locus_tag="PKV49_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPL22215.1"
                     /translation="MLDSKINSDHRSYLQNRGPARQLVRQRLEYYNQFYGFQYGRVSI
                     KNQRSRWGSCSRQGNLNFNYRLVDLPDGLADYIIVHELCHLKEMNHSVRFWRLVAQVM
                     PDYLARRQKLKKIRIN"
     gene            complement(24173..24682)
                     /locus_tag="PKV49_00935"
     CDS             complement(24173..24682)
                     /locus_tag="PKV49_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22216.1"
                     /translation="MKLVITVNDIAVSGVGSDSPYIEDYSEGTFNSANQEAAKISRKI
                     FYDFLNEVKRNRFFKQKKVKEPLVNFFDLWIEVVDQAERCGGIGELERLVNDVFEDNF
                     TPWLGFHKNLVRAYMADRFQGVMSERAKALWHEVISSDNMRRRLANYDIVVTKEYMEM
                     FPKANQLYR"
     gene            complement(24910..26163)
                     /locus_tag="PKV49_00940"
     CDS             complement(24910..26163)
                     /locus_tag="PKV49_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22217.1"
                     /translation="MGLEKLSGSEQKIQEYISRIKNGESKDSIMSGLPPSFIAGIESG
                     LNSPKENEPKEEIKSEIPPQYQGLDAETLNFIWTIPEYVDPERTKQEREKKQRAVDSL
                     RNNKDIQKEVQKNKLIDNLKIETIRKEIGTGSDETIAMKQKEIDLFESQHNDEQSLVA
                     GLIKYVSQGRNQAVIDLYQNFFANINNQESRQQLFKGLFQDIYNKYRLADYGIDPHEE
                     QTWNEALNSRNIEINNKKSEWMYRGIFPKNGEETVTRGSLNVNVTPELISAIDNLIIS
                     GKIKANYKFGQPGTSASPSDRHDSISLYFLEQPSNEALEELSQTIKPYVRGDNLLGKK
                     IEDGFFMSEIGSIETEHVESFVNDLKSIDSALAEAIKGYCSPRPGQGNSLKMSEAQYY
                     AIKDVVRAFGYNISYAKDSGFNIAN"
     gene            complement(26382..26831)
                     /locus_tag="PKV49_00945"
     CDS             complement(26382..26831)
                     /locus_tag="PKV49_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22218.1"
                     /translation="MKKFFLLVTIIATMLLGTGCEKKRLLPWLKIDGGFTLYVQGSGI
                     RNYQGSTYWDRYYLISEPFDSRFNLELPDRPEELVYKDFDQDGIKDIAITSLYGNLAG
                     YWRVTKVAFGRKNGTFDKEFQELPHELLHKQKIKVQEGSKKSLDYGS"
     gene            complement(27006..27695)
                     /locus_tag="PKV49_00950"
     CDS             complement(27006..27695)
                     /locus_tag="PKV49_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPL22219.1"
                     /translation="MAIIVGHKGAAGYAPENTLTSFREAIKIGCDRTELDVRLTNDKQ
                     VVVIHDKEVSKLTDGTGLVGEMNLLELKKLRCLGNESIPTLQEVINICKDKIDLQIEL
                     KAEGTPQPVSNLITQNGIEDQVFITSFKINLLAEIKKLNPGLRVGLLFRDEEVMADIW
                     NLINQIPLDFLAPFSEIITAEFVAKAHSLGRKVYAYHVNNKELGDKLIAMGVDEIGTD
                     FPKLFIEPKIV"
     gene            complement(27705..28028)
                     /locus_tag="PKV49_00955"
     CDS             complement(27705..28028)
                     /locus_tag="PKV49_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPL22220.1"
                     /translation="MKTYNKLIRDRIPEIMEARGVKFKTSILSQDDYKNELLNKLVEE
                     SREVLAANTDRSELIKELGDVFEVIDYLIIAFGLDSEEIGAVKAERKESRGGFTERVF
                     LEYTE"
     gene            complement(28180..28452)
                     /locus_tag="PKV49_00960"
     CDS             complement(28180..28452)
                     /locus_tag="PKV49_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HPL22221.1"
                     /translation="MFSLKYSSRFKKDLKSYRHQEGLFIELEKVLDILVGGGVLPQKN
                     SNHPLIGEFQGCFECHIRPDVLLIYKIEKAELLVLLLRIGSHSDLF"
     gene            complement(28456..28740)
                     /locus_tag="PKV49_00965"
     CDS             complement(28456..28740)
                     /locus_tag="PKV49_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HPL22222.1"
                     /translation="MIAKNSEQIQIRIDVKTKQETMKVLDSLGIDMSSAVRLFFRQII
                     NAKNIPFELRDENGLTLRGAEILRESIIGAKSSKKSFTSGANLIKDALRS"
     gene            complement(28835..29494)
                     /locus_tag="PKV49_00970"
     CDS             complement(28835..29494)
                     /locus_tag="PKV49_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HPL22223.1"
                     /translation="MKLLLTSAGISNESIAKALFDLVGKKPQETSLVFIPTASNIENG
                     DKGWLIDDLTNLRKQNFKSIEIADISAIDKIIWRPRLKSADILYFEGGDNFYLMEWIN
                     KSGLGRLLPQLLVNKVYVGVSAGSMVISKDLNLNSLQILYQENLDRTEDLLGLNLVDF
                     YFFPHLNSPHFPHVRPDLIKEFAQKQTDKIYALDDNSALKVIDDSVEIISEGKWLVFN
                     D"
     gene            complement(29638..30111)
                     /locus_tag="PKV49_00975"
     CDS             complement(29638..30111)
                     /locus_tag="PKV49_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22224.1"
                     /translation="MSNLDFTRQLVKGKIAETIFSQMFRGSNNFTVLEFGYEKVIPEM
                     IQQGYQENNPMIETLRTAPDFAVIDRETRQVKLIEVKYQRQLSLEYTLKAATRMSQSW
                     NPSYLFIATLDGFYLDEITNIIDNNGQIAPLGDSHIALELQEDYLQILRRFEQGN"
     gene            complement(30133..30843)
                     /locus_tag="PKV49_00980"
     CDS             complement(30133..30843)
                     /locus_tag="PKV49_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HPL22225.1"
                     /translation="MGSFVAFVLSIVLGLKHSPQFIGKNTECFVNKKLSKLNHDYYKV
                     LSDVLLPSAGNQIGTTQIDQIVVSNFGIFCIETKAYSGWIFGRAEDKYWTQVIYHKKS
                     RFYNPLWQNFGHVKTIENLIATLNIKVPVYSYVAFPDADNLRITGTDLVGYAGEVVKK
                     IKFHNQFVLSNIDRDRIVDEINQINIVDKNIRKEHIKKINEIIDAPPPVRYKNNEQVQ
                     GEIENIFDDDDEEYDDDF"
     gene            complement(30998..31258)
                     /locus_tag="PKV49_00985"
     CDS             complement(30998..31258)
                     /locus_tag="PKV49_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22226.1"
                     /translation="MEKKKTIKKILAIILSVMLVLIGLYFGIIGALVGASLAGLLLQK
                     MNVVSSDSVWVKETKSLKIAKYVILFLVLAAIITAFAFIISS"
     gene            complement(31285..32235)
                     /locus_tag="PKV49_00990"
     CDS             complement(31285..32235)
                     /locus_tag="PKV49_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPL22227.1"
                     /translation="MRCHKCNFELSENANFCKKCGASISSRKNNFFSKIKKYKWMLVA
                     VFAVILIIIFSTLNSEPSNNTEKQTVNQTTEKAELENYFEENLKKSVVNIICSTKDDK
                     YFDKAETGGSGTMLSSTGVVLTNFHIMPYAKPGDLDNIRCMVVLSEPKLGSPAEAYIA
                     KPMVFLDLSKKYDLAFLDIISSYTDEDGYTYGDYEKDFAIYDNSACVNKGDPILGEKI
                     RIFGFPSVSGGKALTITDGLISSFDDNEDIITSAKIDSGNSGGLAVDQNGCIVGVPSA
                     VTVGNYENLGIVISVNNVNRFLKEFREKNDKEGAFNISFE"
     gene            complement(32237..32710)
                     /locus_tag="PKV49_00995"
     CDS             complement(32237..32710)
                     /locus_tag="PKV49_00995"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPL22228.1"
                     /translation="MFFIFGWGHQITKNYGITFKNRCSSCNNEDYWQLLRITTWFTLF
                     FIPVIPYERKYFFMCPVCERGIYLDSDKFEELKTLAEANSDLIDKRITIEEYNQRLAG
                     PKKVEENVAGYIEGEVEQKITDNNDDSISQIFCSECGYKNSIKNNFCQNCGEKMK"
     gene            complement(32714..33241)
                     /locus_tag="PKV49_01000"
     CDS             complement(32714..33241)
                     /locus_tag="PKV49_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPL22229.1"
                     /translation="MDCLKCGFDLPENSKFCPKCGEKVVIPKKEEAKSDKSVCEEINN
                     HLEFLGYKISKEEMSGEQNKSKYIYSCIHPKLNNIVFYKILDNLILFRVNLTTKKKET
                     NEMLKYANVANDKSLNVCKVFIKLDDDKKAQISFEAIYTGHYSKEIFSEFFNEFVDDQ
                     KRFYVGDNFDKIFID"
     gene            complement(33268..34170)
                     /locus_tag="PKV49_01005"
     CDS             complement(33268..34170)
                     /locus_tag="PKV49_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPL22230.1"
                     /translation="MFCKNCGKQIDGNKKFCSSCGASINNIVNDPGISNYKTKGKKNY
                     KDNLVKFIKISLGVLLIGGFLIFKIYNSLDSDVIDTNNQALKNFHEGDSQQAITQFQE
                     ASQGAITDSTKINTLKNLAYVYSTEGQNELALNSFKEALAIATPESFDYYLISGEINL
                     LEKKPNAALANYNTAYKMNPDDFQINNALALFYLDMEEISPQSSDYSKALIYAQKAYD
                     LDGSETSKQNLAIANYFNDNFDKTILLLSSSDLNKNPYTAYWLGLAYVKKEDMDNAKI
                     YLKKAMDGGAEVPQEIKDFVNSND"
     gene            complement(34175..34789)
                     /locus_tag="PKV49_01010"
     CDS             complement(34175..34789)
                     /locus_tag="PKV49_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPL22231.1"
                     /translation="MFCENCGNKIIENTQFCHNCGSKISDGNEDKKAPLQNSKSDNKP
                     MTREEYMAHTNIKSEEEAKEYLHRLNFLFIAVFIGIVIVRGGADSIDEGLYAILWLAD
                     IALLIYFVWYCVKVIKAEKISKASAVFSIIFAPISWFWFYPDITDPLKIILGKKVPPI
                     SLPSNLSSEEKKKKDKKFWRNFWIIMGSIFGALILILILISLLS"
     gene            35214..36137
                     /locus_tag="PKV49_01015"
     CDS             35214..36137
                     /locus_tag="PKV49_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22232.1"
                     /translation="MTLHEIKQKIEILLDKYQKNSGTIKGFDILFEYVDFLKSEPYTA
                     NLLKPQFDYAKDQIAQLDEYIAKNSCFKEVPNVHKIMEFKDVSEAPMHKDVIAVGLEQ
                     LKSQEHANMSQMLPLAITQLVMMYEGMGRVKEQLKNKEEIEDKLNILQELPTSTLRVK
                     VGEELNSLPFSVFAKTNLLISAKYILDKIDIEELSEETKKEPWFDKDNSVLYFQNKQI
                     KITLKNNKPLDHYILEAIFSKNDLNEQTDFVEISEDIMKEEYNGKWEKFRHACDKLNQ
                     KIFKATDNTVSDFIEYTTGKTGWCKVNQKYQ"
     gene            36457..37398
                     /locus_tag="PKV49_01020"
     CDS             36457..37398
                     /locus_tag="PKV49_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22233.1"
                     /translation="MKQRDNKYLSNRKLVKSGSLLEIYQFGRDINLGRKIRHIKTEEE
                     KALQNGRPKVSTEEKLKSSARRARRMIKRLIMANCFLWFKDKGKPYLPITLTLTFAKN
                     ITNLKKANYEFTKFIRRLNYETNAIEGKDLKQSNLKYLAVFELQKRKAIHYHMIFFNL
                     PYIKDVYRKMYKIWGQGRIMVGGKDRNFNKVKDQAQLKKIIFYFIKYIQKSVFENDFP
                     RQKKYIASKGLLKPQENSFVEVVSLVESKLSDKQLVYKYDGEMEYNNSDIRDTVGQPQ
                     NFLRWFNYQQYDLSDNPDLDKEISQLLAGLSIDDIDF"
     gene            complement(37623..37943)
                     /locus_tag="PKV49_01025"
     CDS             complement(37623..37943)
                     /locus_tag="PKV49_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22234.1"
                     /translation="MLDFLKEGLSSPVVLVLTIGYLITSSITTFDIRMTQGRKNGTLP
                     ANEPMLPNWVAYIFWIDWIIIIALAFINWKFAIAIFIIRFILKVLPVLEILGNFLMAP
                     FKKK"
     gene            37994..39499
                     /locus_tag="PKV49_01030"
     CDS             37994..39499
                     /locus_tag="PKV49_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPL22235.1"
                     /translation="MTTNDQTIKYALYCRKSTEDSSRQILSLDSQEKEMIKLAEGLDL
                     TVIKTFRESKSAKKPDNRPQFTELINLIKRGQIDGIICWKIDRLSRNPIDSATIQWLL
                     QQNQLKIIQTMERQYLPSDNALLFNVESGMANQYILDLSKNVKRGIKAKLEKGGWPNL
                     APIGYLNKDSKIIIDKARGKYIIKIFELYRTGKYSVKEIAEILFKEGFRSRAGKKYHK
                     SKIHKMLSESFYYGIMTKDGQLYEGKHEPLISKQLFDEVQEMLNQKAHIKKQNIPFAF
                     RGILRCAKCGCLLTATKKKGRLTYYYCTNGKNICEEHKAYIKEEYLSKELNTIFDKLV
                     FNEKIVNLVYKAKLKESTSAISYLDEVILNLDNRAKTLNERKKVLLDSYLDKIVLEGD
                     YRAKSQAIENELVLITKDVEELKIKKLNQDNTTLERIKNFFLAPKQMKKEFLRSLPEK
                     QRDTLIGLLWNAEVENKKIANISFKQPYDAMSKIENKSDFSSVRRRRDSNP"
     gene            complement(39473..39559)
                     /locus_tag="PKV49_01035"
     tRNA            complement(39473..39559)
                     /locus_tag="PKV49_01035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39523..39525),aa:Ser,seq:cga)
     gene            complement(39567..40304)
                     /locus_tag="PKV49_01040"
     CDS             complement(39567..40304)
                     /locus_tag="PKV49_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPL22236.1"
                     /translation="MINQQLTKYLASHGPWSRRQAEALIRAGRVKVDNQIAQLGQVVS
                     GDNQTVQVDGRLVASQDEPMVYFMLNKPAGYTCTSRDFRDEHNVFELVDYPGRLFVVG
                     RLDKDSRGLVLLTNDGELTERLTHPRFAHAKVYEATTRPGLIGRDLPKLKDFLAKGRR
                     LYDDQGLATAEVGQQLATDRLEIILRQGQKRQIRLLMKEAGYEVTDLKRVALGSLKLG
                     RLPVGACRPLTDIEIDRLFHLAQDRRV"
     gene            complement(40297..41655)
                     /locus_tag="PKV49_01045"
     CDS             complement(40297..41655)
                     /locus_tag="PKV49_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPL22237.1"
                     /translation="MWLIALLVGVFWSILAWRRWPLAVSLLAGLLPTYLLRFKVGPLP
                     MTILEELILLTTAIWLIKQYPQPWANLRFRAKNWRAYPLTIEITLVLLIAWLALLIGG
                     NTASALGLWKAYFIEPILVFILFWNVPRGTKYIDCGYLYLAGSLLVVGLIAGYQYWTG
                     NLIANSFWAAAATRRATSVFGYPNAIGLFAGPVSLTLLGYLIYRLKKVEGWLNWSNLL
                     LISSIILGWLAIYWAHSEGAMIGLAAALVVLGLLTNRWSRLVVLVVTVVAGIIIGQRP
                     DYQATIEQKVRLADLSGQIRQSQWQETWQMLSDGRWLTGAGLANYQATVEPYHHEGIW
                     IKSADKKTKPSWQPLEIYLYPHNIFLNFWSELGLVGLIIFVWLWLKAALISLINFWRY
                     RRQKLAWLSWGLLGAWLAAAIHGLVDVPYFKNDLAVLFWLGLALTIWLKWQLTDNKEQ
                     ND"
     gene            complement(41657..42151)
                     /locus_tag="PKV49_01050"
     CDS             complement(41657..42151)
                     /locus_tag="PKV49_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HPL22238.1"
                     /translation="MLAVVSDLHDNLINLQLWAQWCQSEGIQEAVFCGDLTDSDTFQQ
                     LASLWPGQLYFVQGNADNYAPADIPSRPNFYNLGRHGRFVINNRTIGVCHEPEYQDIV
                     LNDGPVDVLFYGHTHKPWQEQSDNIWRVNPGTLGGVYYPATFALYDPLNNTWVLKNLQ
                     QLDK"
     gene            complement(42153..45179)
                     /locus_tag="PKV49_01055"
     CDS             complement(42153..45179)
                     /locus_tag="PKV49_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPL22239.1"
                     /translation="MVENNHLSIEDLLNDKALASKDSPAAQLSQRQSDLRYQAIEDQV
                     AAQAQAQGLPYINLLNFPISPEAISLLDRNDATDLAAVCFYYEKNIVRIGTTQPSEAL
                     TAKIAELSANNRLKIKLCLISQRSLEYAHKIYDTLPKITRLEGVEIDTEELARYQAEL
                     SEMSLLNNKLKEANISQILTLVIAAALRAESSDIHIEAEETAVIVRLRVDGLLQQVAT
                     IPRQQWKKVLSRLKVVARVKLNVEDKPQDGRFDIFLPDEKVAVRSSFLPTAYGESVVM
                     RLLRPKSAVLPLDSLGMRPEVRQILENEMAKPNGLVLTTGPTGSGKTTTLYAVLNKLN
                     QPGVKIITLEDPIEYELQGINQSQVDEKSGYTFANGLKSILRQDPNIVMIGEVRDLDT
                     AEIAVQASLTGHLVFSTLHTNDAAGVLPRLVDLGVQPYQLVPAINAIMGQRLVRKLCP
                     QCRRVHQPNEAERQQINKILAVISPKANIDVPKELPTIYEAGDGCPACSGFGYKGRIG
                     IYEIFTMSEAMKELIADGVPSFKIMRQAIEDGMVTMLQDGILKVLDGQTSFDEVLRVI
                     GKTDYIDELYDIVVSQTLGRGLKISEDNLKFGESVAQDIVGAEKLLANKPTSEMLASV
                     MAGAIVSKAADVHIDPIEGGVKIRYRLDGILHDIVTLGSEHYVPLIGEIKNLIGLSLS
                     VKQPTFDGRFSMDLPAERMDCRVSLITGGYGETVVIRLLSNQAISLELADLGIRDASL
                     AAIEKSMHKTKGIIITTGPTGSGKTTTLYALLKRLNSPEIKIITIEDPIEYHLDGLMQ
                     TQIDTAGGYTFAAALRSLMRQNPNIIMVGEIRDDETAKTAIEASLTGHLVLSTIHANS
                     AAGAIARFAGLGVERTMLASALECSIGQRLARRLCSKCKQAVNISPEMQTQIEPFWQL
                     LKTRPELKLPDQPQFYGPVGCPECRGIGYKGRVGIYEVIVMTPELQKLIQQPLTTDAE
                     IEQLAISQGALLMTHDGLLKAANGETSLDEIWRVAN"
     gene            complement(45184..46890)
                     /locus_tag="PKV49_01060"
     CDS             complement(45184..46890)
                     /locus_tag="PKV49_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22240.1"
                     /translation="MLNPKIHKRSVVTLTVNDSTKKLLRAIYRAQHSSGAAKGDQPRI
                     KVSSVISRMSFFYEKIRNAVDYKEEYLLRKEAIERILRRQILIESTIAANDHEKISQH
                     LLAELIRGGYLPNNYLPTSLIDEVAVIIRKYLVIKDIAVAQLDHQAEPTSLIKKDLVK
                     FKEELSQRNDLVAWLLAVAASEIESRLGNDQALNQAINNLYQYLLDNIELANHPELSA
                     DLPIQIYLSVNRNFAKLDNDMLAFILFKYYLANWQKLEASRWPAVAKKINEIRQAINQ
                     QLQHPLAPALDKLTRRYAVCTSVLLDVLQSDPVGIYTSIKTDPKAFPRQIKKVCEKNY
                     AKAKNLLWRAAVRSIIYIFLTKTILVIALEIPANKIFGASVSVMALLINALFPAALLF
                     FSVLITKLPNESNTNKIISIVNELSFEENRRETPIIVRLPQKKAGILTKFFNIIYALT
                     FLVTFSAIVWLLHILHFTLIGIIIFLFFLVFVSFFIIRIKRVTNELKITEEKESLWRI
                     LFDIFTLPVMAVGKWLSEKFSKVNIFVFLLDFIIETPFKFFIDMAEDWTNYVKERKSQ
                     IN"
     gene            complement(46955..48910)
                     /locus_tag="PKV49_01065"
     CDS             complement(46955..48910)
                     /locus_tag="PKV49_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22241.1"
                     /translation="MPERPELMINSSQFTRGFSKDHAQSLRNETASKIRETRNRYRNS
                     SMTYQEIKASNDQVLIDAYDRLKNNFWGKIFKSEKYLADKKLLDFAISRLKNNQMKDC
                     LVEIKNKYNQAIVELMQKTPLTKDEQDRLLSADNIAKMNLADYLSLANRLSGLYFSHV
                     TRFGLRETVGVHCGGQGQVIDGFKPILESGRLGSLVDHILSKADYAKIYIREDLLSSY
                     WRTKRPIDLPKDEAVKMFMHDFFNRELFAACPKPDCSATHFGYNHILEDYGAEAGYTI
                     YFYFPVEVIAKNFQHNLNPEHPRSSQKDQHNDQVAWSAGEGIPLDLGVVCIPENVPVD
                     KASGSKYVLDKNRQPTVCSECRDQLTEINQARLEFNSALTKIRNDYWSWKESGPSLPS
                     RDIEFFDRPEVIELARKFGFIKLEEQLIRDDREYAYGCLPYSFSSSNLFSPQHPGGHG
                     EKAMAYFKANKTTLFKDFVTAFRFDSNEPADQLLSFYLNSEIYQRFPDKNIFRLFLER
                     PSADNTISSKEYWENYFNANPQTRPSKILYYDRTDMFTGPDFKNKPLLKTEYYAINED
                     NSGAHYKELISQLRFKDTIPGTQEFFDKYNVDEFLDHPSYRDRDKYLAEHAHYPSGYL
                     DFWKQVEPKIADFVGEIYDEIKADQGQ"
     gene            complement(49026..51119)
                     /locus_tag="PKV49_01070"
     CDS             complement(49026..51119)
                     /locus_tag="PKV49_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22242.1"
                     /translation="MSDPPNQKLEQPNIEAGKTPEQLNVSEKSSIERRQELLARIDDE
                     LSVLPKLDKNIDLVRDEKENETTRQIKDIENSLGQLLSADSKKQIMHNNIDMTIEKIV
                     KSQETREQLEKQKNILEYIAKELPKTEARIAEPMRQECKVAVSIPAYEEGGKILNSLE
                     FLANQKDVSMDDYEIIVNVNNREEAGNNTRHLNMQTLSVLKFLKGEPSDLAFLTTEET
                     ERLQKIKESGLVVHYIDKSSGGNEIKPIDDEDFKKIGCMQPRKRTMDEICERFVLAGK
                     TDGIIASLDADTKVNSQWIRGIIDAFNNESVQLLAGERRDDFDISLESEQIDRDRVME
                     RLLDDWNSEERINDEACKNLPNIKSRRQRAQILLMNHLIGIYNTARDHFRREMEGKTG
                     VIENFKGGSGKMFRVGTYIRHPLAIIKIMDAGGGKPAEIDESAFQYVGRNKSEKGKYI
                     TPNPQIEALAVYPETRIRGWDLKSEDRDEPYKDFHDIYKPFGSSGKEYAYAIAAVLKE
                     KMPTTYNEEAGDMRMALENFIAGDQTTEARQKLERYFDQKTVSQIFEITPEDLEKIKA
                     GDQTTIAKHRNVVDKIEEFSKKPVGIIDAIKFYGSKNSSEQISNPERYFFGALVKQMM
                     ISQGRDQEQIKNEIKDHYGLSDELIDQVSQLKTQEELLQFSRNNSEIQQILVTMPEKV
                     SYNEAAEKYKKLIKF"
     gene            complement(51436..51512)
                     /locus_tag="PKV49_01075"
     tRNA            complement(51436..51512)
                     /locus_tag="PKV49_01075"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51476..51478),aa:Ala,seq:tgc)
     gene            complement(51517..51780)
                     /locus_tag="PKV49_01080"
     CDS             complement(51517..51780)
                     /locus_tag="PKV49_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22243.1"
                     /translation="MFLLRFLGGLVIVAIGALIIMKREWFLNNFGRMEFFEEKLGIYG
                     GTRLAYLLIGLIAIFIGFSIAFGLINNFMGWLLGPLIRYQKNT"
     gene            complement(51784..53103)
                     /locus_tag="PKV49_01085"
     CDS             complement(51784..53103)
                     /locus_tag="PKV49_01085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPL22244.1"
                     /translation="MAGLAIKGLILIINILLKVKQISFWLFSRLLKLFNWLIIYQPAV
                     WLYRGYLRIKRRLINHYQQNLWLTVTVVAVVAIGVVFGNWRASGRLTAAEDLVRGTWL
                     SKLVGSEFNQLNNFVVDDRAGYQLAPNQSTEVSAKLSTLGISDPGPAGLVGMATDRWV
                     YSLGLASLPSSNQPAAGAAGGRRSITYYTVKIGDTLSGVAKAFGVTVNTIAWQNNLNA
                     KALLRPGDKLVILPVSGVLYKVRPGDSLSKIAQQYKVEAAAIISYNNLTGDRVIIGQS
                     LIIPGGQPPASVASQVAVASSKLTPKVQQVFSNVKNTVKANLGTGGMTWPTVGHRITQ
                     YYSWRHNGVDIGNKVGTPIYAADGGKVEVAANGWNGGYGNTILLNHGKLKTRYGHLSR
                     LYVRAGQTVVKGQVIGEMGSTGRSTGPHLHFEVLSGKVRYNPLNYIR"
     gene            53275..53347
                     /locus_tag="PKV49_01090"
     tRNA            53275..53347
                     /locus_tag="PKV49_01090"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53308..53310,aa:Cys,seq:gca)
     gene            53850..53921
                     /locus_tag="PKV49_01095"
     tRNA            53850..53921
                     /locus_tag="PKV49_01095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53883..53885,aa:Arg,seq:cct)
     gene            53946..54019
                     /locus_tag="PKV49_01100"
     tRNA            53946..54019
                     /locus_tag="PKV49_01100"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53980..53982,aa:Ala,seq:ggc)
BASE COUNT        17023 a        11710 c         9535 g        15752 t
ORIGIN      
        1 gaagacgatt ctgctcttgc agtaatcgcg cctctggagt ggtggaagaa tcggtagttg
       61 tggcaatttt ttctaataat tcactacctt tattgacttc ttctagcccg gcatctaaat
      121 cctgacgaat ttgattaata tccaataatg gctgctcctg gtttttttgc ttgctaataa
      181 aagaaagccg taagctataa aaccaaaaga ccaataatat agtcatgata gccgtaatac
      241 caacccaagc aagcaaacgt cgtttctcaa ttggccaatc ggccgggctg gaagccttgt
      301 taataagccg ctgacgcttc ataaatctaa gccgtaactg atagaataat taattgcaaa
      361 ttttcactat ctagatcgcg acgaacaacc ttcaatttat cttcatagat cggagctaag
      421 ccaatagctt taacaaaatc atcgacagca gcaaggttaa aacttaaacc aggtaattta
      481 tagtgcattg gtataactat cttgggctct atttgattaa taacctcaat cgcctgtttg
      541 gcatctaaag tatagtaccc accgactgga accaatagaa tatcagtacc agccaaacgt
      601 tctaattgtt tagcctccaa agtactgccc aaatctccca aatgagtaac tactaaatcg
      661 ctaaaaccca aacggaatat taaattgtgc ccacgctttg caccgccctg ctggtcgtga
      721 tcgctgtcca ctccatcaat aaataaacca ccgatttcat attctccggc catatctata
      781 acaaatggtt gaccgctaac tccagacaaa aaattgtggt ctttgtgctg atggctgatg
      841 gtaaccacat cggccgataa attggccggc aattttaatc cggtatggct gggactaaat
      901 ggatcaagaa ctacagtaat atctcgacca ttcattttgt cttgcaactt gaaacatgat
      961 tgtcctaacc aactaataat cataaaattt atataaagac ataacgaagc ccgttaaccg
     1021 ggctgacttt aatttaacaa aaatcacgcc agctgtcaaa gcgattaagc ttattttaag
     1081 acctaatcag tggattttag acctgacgaa attggcagat agaacgaaaa tcacagtaag
     1141 cacaaagctg actaggttta gctgggaaaa agccctggcg aatcgcctca acctgggcgg
     1201 ctacttcttg ctgaactttg tctaaatcag ctggcttagc cgtaaaactt aaggcttgtc
     1261 cactatttaa ataataaaaa gtcagtttgc caacccgcca accaaaaact gattcaaccg
     1321 ctagctgata aatcattaat tgacgctttg tctgataatc tagcttgtct ttgggcttac
     1381 ccgtcttata atcaataatt tcaaccgtgc catcagctaa ctgatcaacc cgatcaatcg
     1441 tgccgccaag agtgaattgg gcggttttcc aaacaaattt tttctccaaa gccaaaacat
     1501 tgggccaacc accagcctca atctgctgcc aaattaattt taggctctgt tggccaagta
     1561 aacgatattt ttctcggtcc tcagccgaat cataaccatc atcaacccaa ttcttcttat
     1621 agatagccat taatccggcc caatcaggtt tagttggaac tgttgactga ccaaataaat
     1681 cagttggctg ttcgtttaat aaacaaggcg caaacaattc atataaacaa cggtgcatca
     1741 gacgaccata aactatatta cctttatcgg gcactggcac tcttaagaca aaagcaaact
     1801 tatattgtaa tgggcaatta ctataactct caaattgact gaaagaaaac tttttgaccg
     1861 ccactaatgg cgctggcaaa atctgattat tgtcggagtt ttttaaagcc tcaacttcac
     1921 gcactaactc aaaaactcta tctgcatcta actgacgctc tgtctgatta gcagtcagcc
     1981 cgatctcgct aagaaaaatt gacggccgct tatcgcgaac tccgccatag tcacgggcat
     2041 aggccaaaat taatttttct ttagctcggg tgacggctac ataaaatagc cgccgctctt
     2101 cagctaaatg aacgtcaccg gagccaactg gttcagcaat taatccatca ggcaccggca
     2161 aactgtcgcc acgtttggtg gttggaaagc gtttatcaac taaatcgggc aaaaagacat
     2221 agcggaactc tagtcctttg gccgaatgaa tagtcataat tttaaccaca tcatcatctt
     2281 cgagtggcaa acttagacta ccggtttctc cggcctccat ttccatgttc attaaatcaa
     2341 caaaatcctt aagatgtaaa tcaggaccgc tgtcttcaaa ttctttcatc ttcttataca
     2401 gctgattgag ataatgaaaa atcgcgctat ctttctcttg gtctaagcca gccaataatc
     2461 ccgactgata aacgaaatca acaaacaagc ggctaggcga ctgctgaaca gctgacaatg
     2521 actgggcaga gactaacttc aacaacttat ctatagcgcg acgaccgtct gggctaatct
     2581 tgacggctag ggcattgttc tgtagggctt caaagagtga ccaggctttg cgccgagcta
     2641 aagctgacaa ctcaaccaaa tcggctggcg ataaagcaaa cggccgagaa tttaaaactc
     2701 gatataaagc ggttgactca tgataattgt ctaagagctt aaaataagct aaacaatcta
     2761 aaactatcgg cttatagtaa agcccgcgca aagacatgaa ctggcagggt attttttgcc
     2821 gacctagttc attaataaat ttatcggcat cgcgattgga acgaatcaaa atggcaaaat
     2881 ccgaccattt agccgccggg tcttgtcgat actcttcttt aatataatca acaatagcca
     2941 ctatttcttc agactcgctg gctagcggcc aaacctcaac tgaaccatta ggttgaccga
     3001 cagcaactag ccgcttatta atttttaaac gatcttctag ccgatcaggg ttgttctttt
     3061 ggattgattg ataggcttga tccaaaatcg gttgaccgga acgataatta tcggttaaca
     3121 cgatgttaac agccgctggg taatcatctt taaattgtaa aatattggct aaagaggcgc
     3181 cacgaaattt ataaatactt tgatcatcat caccaacgac ggttagatta ttgtcagggg
     3241 cggccaaaag tttgatcaac tcatattgcg cccaattggt atcttgaaac tcgtcaacta
     3301 aaataaattt aaattgctga cgatattgct gtaaaatatt cggcctttgt tggaataacc
     3361 gtaaagtata gctaattaaa tcgccaaagt ctaaacagga attatcaagc aataattgat
     3421 tataagtctg ataggcccga gccaactcct gtaatctggc cacctccaat tgttctggct
     3481 gaccggcatc gcttaaatca agaccatcaa catactgcaa ataatcagct ggcaagatat
     3541 tctcgtcttt gagatggcta aaatatttta ccaggtcgcg caaaaacttt gtctcattgc
     3601 ctagtggacg ataataatcc agctgcagcc gatctaaatt tttgcgcaat aatatccatt
     3661 gaccggtttc attaagcaac ttaaaattag tcgggaggcc aatatctaaa ccatgttgac
     3721 gcagtaatct ttcgcctaaa ccatgaaaag tttgaatcca caggtcagta taaccatagg
     3781 gcaataaacg atcagctcgc tcttccattt cgccagccgc cttgtcagta aaggtagtta
     3841 gcaaaatttg atccggcttg gcctgaccag acatgattaa atgaagcaag cgattaatta
     3901 agacggttgt tttgccagtg ccggcgcccg ctacaattaa taatgggccg gaaccatgct
     3961 caactgcttg tctttgggct gggttaagac cggccagata atcaaagggt tgagccataa
     4021 ttttgttcac taattaaaag acaaatgtgc taataattta tccggctcgg ttacccgccc
     4081 aataatagcc aaattaagcc cctgatcaac aaaaatttgt cgagccacag attgaatttg
     4141 ttcagcagtc accgcccgaa ttttggctaa atattcagtc ggtgtatcca aattttcctg
     4201 gacattgcct tggcctaaaa acagaacctg ctgccgagca taccactgag ccaaatcatc
     4261 cgacccctct aacccaagaa tcaaacggcc agacaagaat tttttgattc gctccaattc
     4321 agccgcatca actaattcag tagttaattt tttatactcg gcaataatag tcgccactgc
     4381 ttgagcaacg gcctctttct taataccggc cgaagttacc agataaccgc tatcggtata
     4441 attttcaaca ctagtccgaa cataataagc taagccttgc cgctcacgaa tttgtaaaaa
     4501 caaacgggaa ctcatcgatc cgccgagaat agcagctagc aaacgactag gtaatctttg
     4561 tggatgacca taaggaaaag ctcgaactcc taaagctaaa tgcaattgat cagtatcttt
     4621 atcagcaact aacaactggg gctgactttg ttgttcaaca actggcagct tatcttgaaa
     4681 attaattgct gctggccatt gagcaaaata tttattcact aaactatcaa tattctcggg
     4741 taactgacca gctaaacaaa taatggtccg ctgctggcca taattctgcc gccaataatt
     4801 aataaagtcg tctcgttgga aagcgctaac tgtttctttg cggccagccg tatcccaacc
     4861 agccggcgta tcaccgtaaa gagtttgttc aaaaacatct tccaagtgca gcattggatt
     4921 gtcctgatac atgttaatct cttcaataat cactccgcgt tcacgatcta tctcctcact
     4981 ggaaaattgt gaatttaaga gcatgtcact aagaacatcc aaagcgacag ccgcttgatc
     5041 agccgcgcat tttacataat aaccggtata ttctttgctg gtaaaagcat tatattcgcc
     5101 gccaatagcg tctaattcac tggaaattgc caaggtggac ggacgcttac ttgtcccctt
     5161 aaaaaacatg tgttccaaaa aatgagacaa cccgctttga ttagcggttt catatttaga
     5221 accgacggca ataaccacta aagcggtgaa agtcggacag ccggccaaag gaatttttaa
     5281 aatcaatggc cgttcattgg atatttgtcg ttgccacata aaacttaata atctaactta
     5341 ctagataaat aactaaccag ctcattaagt tgaacggttg tctgcttcat ggtgtcacgg
     5401 tcgcgcaccg taacggtacc tttttccaga gtatcaaaat caacagttag gcaatatgga
     5461 gtaccaatct cgtcttggcg gcgatagcgt ttgccaacat tgccattgtc atcaaactcg
     5521 caacgccaac gcattttaac ctgatcaaaa acttcttgcg ctgccttgat tagttccggc
     5581 ttattgcgca gcaatggaaa aactgccact ttaactggag ccagcgctgg cttcaatttt
     5641 agaactactc gtttatcatt ttcccccaac tcctcctcgt gataggcctc attcaataac
     5701 atcaagaaaa cgcgaccaac tcccgacgat gattcaataa tatggggaat aaatttttgg
     5761 tcaactgctt gatcgtaata atctaactcc tgaccagaaa atttttgatg ctgagttaaa
     5821 tcatagtcgc cgcgagcatg aatgccttca atctcttgaa aaccaaaagg gaaattatat
     5881 tcaatatcac aagcggcttt ggcatagaaa gctaacttct cgtgctcatg ccagcgtaga
     5941 ttagccggat caatccctaa aatattaata taataatccc agcgacgctg cttccaaaga
     6001 ttaaaatcaa ctaagtcatt agtcggtcgg acaaaatact gcaactccat ctgttcaaac
     6061 tctcgggtgc ggaaaataaa ttgccgggcg gttatttcat tacggaaagc cttgccaatc
     6121 tgagcgatac caaacggcag ccttaaacga gtgctatcca gaatattttt ataattaaca
     6181 taaataccct gacaagtttc gccgcgcaaa taaactggat tgctgtcatt gccagtaaaa
     6241 ttgcctaaat tagaatgcac taataaatta aagtgacgaa cctgagtaaa atcgcgatga
     6301 ccgcattccg gacaaacgac taaatgacga taatcatcaa atagttgatt aatctcagtt
     6361 tctgacatag tgtcgctggc ctcggcgcca accccttcta aaagatgatc aacacgcagc
     6421 cgagtatggc aataacgaca ttcggttaat ggatccgaaa acccggccac atggccactg
     6481 gcctgccata ctcgcggatt cataaagata gcgctatcta ggccgctaat attatcatag
     6541 cgataaacta attcccgcca ccaaatctgt ttaatgttgt tgataagctc aactccatag
     6601 gggccgtaat cataaactgc ggccaatccg ccataaatct ccgatgacgg ataaacaaaa
     6661 ccgcgacgtt tacagaagct gactaaagac tctaaagtta cttcggacat aagataaaat
     6721 aaggcattaa ggccttttta gtctatcact ggacttcatt tttggcaatc aattgggtaa
     6781 aaattattat caagcaataa aaccttgaca agactagtta aaaactttaa cctaacgaca
     6841 gtcctaacgg acaaataaaa aagaggaggc ttagaatgag cttcgaagaa aaagtaatcg
     6901 tatcgggggc catcatctgg tcgaagtcat ttcatttcgt ggttaaaaac tgcgatggac
     6961 aagagataat gttaaaggca ggcgccattg aggcgtctgc aaagtcaaga ttacaagtgg
     7021 cgcgaattgg ctgcgccgaa tgtcagttta ttactgagat tctaaaaaaa aggccaatag
     7081 agttaagccg agaagatatt acaaatttaa atttctcggc gattttggag gttctgcctc
     7141 caatgaaaaa aaagcgcaag aaggaaccca aaaaacgtcg ctagcgacgc tggttccaat
     7201 aagctttggc tgacacttcg gtgtcagcct ttttttatcc aaaattaaaa ccgccagctc
     7261 gtcagccagc ggctttaacc cctccttatc atcaactagc tgatggctta agcaagccgg
     7321 cctgacgaaa actataaaaa ccacggcggc taaaaataac attatctaat aattgtaaac
     7381 caaacgcctg acacataatg atcagtttat tgaacaacaa caaatctaat tcgttaggct
     7441 cggcaccgcc aaccaaatga ttactggcgg taatcaattg ctgagccggt aattgcaata
     7501 tcggttcaaa aatctgttgc ggatgaaaat acggttgctt taattgttca aataaaaaat
     7561 tcttgcgccg caattcatga caacgacgat ccaaataacc aatcgccagt ctttcttgcg
     7621 tgcaagaacg caaatattta aactcttggt aaataacttc ctggctataa taaatatttt
     7681 gtactcgttg acgacaaaat cgacgagtca taccagtact agctaacacc aaattagctt
     7741 gttgactatt cagcccagct gttaccaatt cttcaaagct agccctcact aagcatggcg
     7801 ctggaattct cttaagaata attttagcca ggggaataca tgctttaata tcttggccct
     7861 gactaataat aaaggctagc agctcactat tactaaattt agctaggtca aactttagcc
     7921 agctagccgc atccggtaat tgggccggaa tctggcagat agacataacg gctagttaaa
     7981 aactaaattg aggaggctag ttaagttaaa aataatagtt tgaccaagtc ccatcataac
     8041 atttttgctt aagcaaaaaa agacaagccc caccttagag acttgtctaa atattaacac
     8101 tataaaatcg ggatattagc aattagccaa ggtgtggacg gcttgaatta tccccagatc
     8161 gtgaactaaa atgacgtggt cccggaaagc cagttggacg ctcggtgggc aatcctttgg
     8221 ctttataacg ttcccaaata gcctgattat ctgacaaatc tttcattgtt aaattaactc
     8281 ggccttgatc atcaatcgct ttaatcttga ccgtaacagt atcaccctcg tgcagcatat
     8341 ccgctggctt accaacataa aatggcgcca actcgctggc atgaaccatg ccatcacgat
     8401 taggtaataa ttctacaaaa gctccaaaat ctaataaacg gacaaccttg ccagtaaatt
     8461 tttcaccgac ttctagtttg cgagcaatat cttgcaccca tttaaccgct tgattcaatt
     8521 tttcctgatt agagccgcaa accataactt gtccattttg ttcaatatca atggtcactt
     8581 cacaagcagc gataatttca ttaatcactt tgccgccggc gccaataact tcacgaattt
     8641 tatcgacatc aataaaaaag ctgacaatct tgggagcata tggcgataat tcggcccgcg
     8701 gttcagagat ggcctgattc ataacctcta aaatctgtag acggccacgg cggctttgct
     8761 caatggtcgt ctgaataatt tccttagtca aaccgtcggt tttagtatct agttggatgg
     8821 cagtaatacc ttcagtcgtg ccggtaatct taaaatccat accgccttgg ccatcttcca
     8881 agtcttgaat atcagtcaaa actttccatt gactcatatc ttgattagag gccaacccca
     8941 tagctagacc ggcaactggt cgtttaatcg gcacaccggc atccatcagt gatagtgatg
     9001 aggcacaagt tgaacccatt gacgatgaac cattagagct aagtgtttcg ctgacaaccc
     9061 tgattgtata aggaaaatcc gctttgcttg gtaaaaccgg cactagggcc ttctccgcca
     9121 aggctccatg accaacctcg cggcgacccg ccgtcctaat cggcttagct tcaccggtgc
     9181 aatatggcgc aaaattataa tgatgcatat agctcttggt cgaaacaccc tccaagccct
     9241 ctaaagactg ctcatcgcta ggagcaccca aggtgacaat agacattacc tgtgtctcgc
     9301 cgcgagagaa taatccagcg ccatgattac gaggtaaaat actgacatca gccgacaatt
     9361 gacgaatctc atctaaagcg cgaccatcaa cccgacggtc tgattgtaaa atattattag
     9421 taacagctgc ctcaaccgcg acttctacgg tttgaccgat aacccattga cgcaggcttc
     9481 tatcaaaatt ctgactaaac aaatattcgt ccaatccatc tttgatagct tcaacagcta
     9541 atttacgctg ccccttggta taataaattt tatcaaataa aattggctca atatgctcat
     9601 ctaaccattg ctcggcaatc ttctgagctt gctgcttgcg ctcttgagcc tgtaattcat
     9661 cggccgataa acgcactgtt ttagcctttt tcggctcaat tggtccgatt gcttgataca
     9721 gttcttcaat taattcaatt gaaccggcta gggcttgttg cccagccata atagcttcaa
     9781 acatagcggc ttcgggaatt tcttgagcgc cagcttcaat cataataact ttactagctg
     9841 ttccggcgac aattaaatcc aaatcactaa tttcgcgctg tttataagtc gggttaaaaa
     9901 ccaattgatt atcaatccgc ccgacccgaa taccgcctat cggtccacgc caattgattc
     9961 ccgaaatccg caaagccgct gaagcagcta ctaaagcaac gatatcataa tcattggttt
    10021 gatcaaccga caaaactgtc aaaattacct gcacatcacg ccgcgatgat tcatcaaaca
    10081 gcggacgaat tgaccgatca atcattcgac cgctaagaat agcttcatca gttggtcgcc
    10141 cctcgcgttt gacccagcgg gaacccttaa taatgccagc agcataaaga cgctcctcaa
    10201 aatcaaccat taatggaaaa aaatcaccgt cttcacgcgc ctccttggcc tccaccacgg
    10261 tagccataac tacggtgtcg ccatattgga tagtaactgc cgcatcagcc tgatgagcca
    10321 agcggccagt tttaatagtt agcggacggc catgccaatc gcggctaacc acaatttctt
    10381 gcttttgcat agaatttaga gctgtcctga tcaacaaact aaaggactat gattatttca
    10441 tagtgctcac tgtttgtcga ccaaaaatca gctaattatt tattaattta ttataaaatc
    10501 aacgactgat taaaaattga cgatcactaa gattaataaa gtcatcagca atctccaata
    10561 atttagccga ggtagtttga cgaaaaccag ctatttctac caaacagcca gtagtgcttt
    10621 gaatataatt aaccagtgga gtgtagtcgc catcaccgct agctaaaaca ataacatcca
    10681 gtttgtgaga taatttaata gcatcaatgc tgataccgac atcccaatca ccttttttgg
    10741 ctccgccagc aaaaacctgc agatctttca taacaacctc gaaaccctgc tggctcaaag
    10801 cctcaaaaaa agctgtttca tcgtcattat tatcactgcg gacaacatag gccgccgccc
    10861 taatcaattt acggtcagca acaacagccg ctaaaacttt gcgaaaatta actttcttat
    10921 tatagagatt gcgagcggaa tgatagaggt tagcaacatc aaccaaaact ccaacgcgct
    10981 gctccgggtg tttaaccatg aaaaatattt taaacttctt cagctaaatc gtcagaaaaa
    11041 tcttcttctt caacattatc ggcttctttg gctaacaatt cattctccag acgcttgcgc
    11101 tcttcttctt cagcggccat ttttttggca atctttagat taagcttagc cgccaaatca
    11161 ataaaggctt tatcatcttc tttttgcaga tactttaata ggcgccgacg ttcaccaact
    11221 ttacgcaata aaccgcgacg agaagaaaaa tcgtggcgat gtttttgtaa atgatcggtc
    11281 agctgcttaa tctcttcggt caagatagca atctgaacct gagttgaacc agtgtcgcca
    11341 tcatgctgcc gaaacttttc aataatttct tgcttgaatt gtttgtctaa catagttttc
    11401 tgtcccccaa ccgagaaaca gtcaactgtt taggccgctc caagaagggg taaaataata
    11461 attattggct aaataaagct cttctgttat aacactgttg gaaaataaaa tcaagatgtc
    11521 gtataatatt atcaatgctt aaagatgata tcaaccattt tttagaatat ttagaagttg
    11581 aacgcggcct atccaataac actttgatcg gctatcgctt ttatttgcaa cgctttttgg
    11641 cgtttgccac tgccagcggc atcaataaac ccaatcaaat aagccaagaa ttgatccatc
    11701 gttaccgcct tcatctcaac cgacttgcta atcgcgagca tgataatctt aaaaaaaata
    11761 ctcaaaacta ccacttaatt gctctgcggc aactgcttaa atattttagt aaacacaata
    11821 ttgaaagcct ggattccaac aaagttgaat tggctaaaca ggaaccgcga caagttagtt
    11881 ttttagaagg atatgatttg accgcctttt tacaggcccc cctaactatt aaaaattcca
    11941 gtaagttagt ccgcttacgc gatcgagcta ttttagaatt gcttttctca accggcctgc
    12001 gggtcagcga attagctcga ttaaaaatta atgacatcaa tttaaagaaa gcagaattta
    12061 ccgtccgcgg taaaggcaat aaactacgat tggtcttttt atccgacgac gctaaagcgg
    12121 ccatcaaatc ctatttagag gctcgccaag acactgaacc ctttcttttt atcagtcatg
    12181 gcaaaatcaa tcaagaaact aatccggtct acggtctaac accacgatca attgaacggc
    12241 tagtgggaaa atacgcctta gcggccggca ttactaaaaa agtcactcca cataccctgc
    12301 gccatagttt tgcaactgat ttattgatgg gcggcgctga tatccgcagc gtccaacaat
    12361 tactaggtca cgcttcaatt accaccactc aagtctatac tcatgtcacc gaccaacagc
    12421 tgcgccaggt ttatcaaaat tttcatggta aaagtcgcaa aaatttacgc caatcataaa
    12481 ctaatctaga cttttacatt taaattgtta taataagcca gctaactaaa taaaaatata
    12541 tttatatgcc cttaattcca accgtcattg aaactactgg ccaaatagag cgagcctatg
    12601 atatttattc ccgcctattg aaagaccgta ttattttttt gggtgaaccg attgatgatc
    12661 ataccgctaa tctaattatt gcccagttat tatttctaga tgccgaaaat agcagcaagg
    12721 atattaaatt ttatattaat actcctggcg gatcagtaac ggccggcctg gcaatttatg
    12781 ataccatgca atttattaag gccgatgtca gcacaatctg cgttggtatg gctgcttcaa
    12841 tgggggcggt tttgttagct gctggagcac caggcaaacg cctatcgcta cccaatagcg
    12901 aaattatgat ccatcaagtg atgggtggtt ttgaaggaca ggctacagat attaaaatcc
    12961 gctccgagca tattctgcac atcaaagatc gtttaaacaa aattttagct caacatacta
    13021 aacaagacct caaaactatt gagcgagaca ctgagcgcga tcattattta accccaaccg
    13081 aggccaagaa ttatcacctg attgacaaaa ttatcagtcg ttaattgagc ttgccttttt
    13141 ttggtcgccg tgttaggata aatccagttt atcttatatt atttatataa tcccgtgttt
    13201 tggttttgtc ggcaacggta gtattctagc tgatatcttg tctatccaca tctccctctt
    13261 aaaaatattt tatcttctat actgtctagc tgtcatcggc tgaatcgtta ctggctccaa
    13321 ccaaagccgc cactattatt atgaattatt ttattggttt aatcgcgcta gctatcggtg
    13381 gtagttttgg ctattggctg cgtcgccaag tagctatcaa caaagccact agcgccgaaa
    13441 ataaagcgga aaaaatcatt gatgaggcta aagttaaaga aaaagaaatc ctcttaaaag
    13501 cccaagataa agcgttaaag ctaattgatg aagccaagca agaaaatgac agccatcgca
    13561 aagaaattac tgcttggcaa aatcgcttag agaaacggga aactgctttt tcccaaaaaa
    13621 tcatggagct gcaagacaag caacaacagc tttatgataa aattaatcaa gtcgaaacca
    13681 ctaaagaaga aatcaaccaa attaagtcgg aacaaactgc caaattagag gagatagccg
    13741 ggcttaatat tgaacaggca aaagaattat tattagaaaa aattaaaacc gaggcctcgg
    13801 atagcttaat gtcgcgctta gataaactaa ataatgaaaa cagtgaacaa atcgaagaaa
    13861 aatctagaga gctaatggcg gcagctatgc aacgattagc ctctactttt acggccgaag
    13921 tcactaccac cgtagtagat ttgcccaacg atgagatgaa aggacgaatt attggtcgcg
    13981 agggccgtaa tattaagact attgaacaat taaccggtgt tgaaataatt gttgatgaca
    14041 cccctaatgc gattactatt tccggtttct cgggaattcg gcgacagatt gccaagcgag
    14101 ctttagatta tttaattaaa gatggtcgca tccacccgac caaaattgaa gatgccattg
    14161 agcatgctaa acaagactta gctctagata ttaagaaggc tggcgaggat gctctttatg
    14221 aactaggcat taccggcttt gacagcaagt tgatccagat tatcggtcga ttaaaatatc
    14281 gcaccagcta tggacaaaac gcgcttaggc acagtgtgga agttgctcat ctatctggcc
    14341 tgttagccga agaattggga gctgatgcca ctctggccaa aaaagccggt ttactccacg
    14401 atatcggcaa ggccgttgat cacgaagtca aaggtacgca tccacaaatc ggtggtaata
    14461 tcgctcgtaa atttagcttg tcccaagaaa ttattgaccc gattgaaact catcatgatg
    14521 accatccgaa gggtttaatt tcggttattg ttaaagtggc tgacgccatc tcatcatcac
    14581 ggccaggtgc ccgcaatgac agccttgacc aatatatcca gcgcttggaa gaattagaaa
    14641 aaatcgctaa tagttttgat ggcgttgata aaacttacgc cattcaagct ggtcgtgagg
    14701 tgcgcgtttt tgttcgccca gaagaaataa ctgatttaga cgcccacgaa ttatcggtaa
    14761 agatagccaa acagattgaa caagagttaa aatatccggg tgaaataaaa atcaatgtta
    14821 tccgcgaatt gcgtattatt gaatacgccc gctaaaaaat cccatgtcga tcggctcaat
    14881 aattagaaag attaaaaagt ggcgtccggt aactaaacgc caacctgttc accatatctt
    14941 agatcagcaa atagtttttc gtctatccaa ggggcgttta ccgggctggc gccaatggcg
    15001 ctacctgccc cgctttctct cgcacaaaga aaaaataatt attatcgcct gtttagtcac
    15061 attgctggct agcctaacta ttattactaa tcattactat cgacatcatc ggcaagtaat
    15121 tcctaaaaaa ggcggctact atgttgaggg tctaattggc acgcctcgtt atattaatcc
    15181 actatactca tcaatgaatc aaactgatcg cgacctaagc cgtttgattt attccagcct
    15241 tctaactaga gatcagcagg ggaaattagt taatgacttg gccgagaatt atgaaattag
    15301 ccccgacggt ttaatctata caattaaact cagggacaat gttcaatgga gtaatggcag
    15361 ccgcctgacg gctgacgatg tcgtcttcac ctatcaagcc atcatggaca gcaattacaa
    15421 ttccccgatt aaaaatagtc tgggcggctt agatgtaacc agagttgatg acctaacaat
    15481 tcgtttcact ctgagccaac catacatcgc ctttttagat ttattaacta tcggcatttt
    15541 gccggccgaa atttggagcc aaataccagc tgaaagcgct agtttggccg atctcaatct
    15601 taaaccaatc ggtagcggtc cttatcaatt taaatcactg actaaagatc agctgggaac
    15661 gattaaaaca tatactttaa cgattaatga taaatatttt aaagatcagc catatatcaa
    15721 ggaattaact ttcaaatttt ataacaatta cgaggaatta gtcgccgcgt tgaatgacgg
    15781 ctctattaat ggtgctaatt atttaccaca gacagcagct aaaaaattac tcgctgccaa
    15841 tcgctataat cttagatatt tgggaacacc tcagctagta gctattttta ttaatcaaga
    15901 tgatcaagcc ctaaaaaata aagctctgcg ccaagcgttg gcactggcta ttgacaaaaa
    15961 tcaaatttta ctagagaatg acactctagt tagccatttg gcaaacggcc caatactaaa
    16021 acaaagccaa tattacgacg cgaatttaac tcggcctaat tttgatgtaa acaaagctaa
    16081 tcaattactg actgacgacg gttggcagat aaaaaccatc actgccgacg aagtcgccgc
    16141 cgccgaaaag ggcctagccg acaatcaggg cgatcaagac caacaaaaaa caatcaccga
    16201 cttgggggtt ggtcagtggc gacaaaaaaa caatcagtat ctgaccatca atttaacaac
    16261 cattaatgaa ccggattatc tgctgattgc caaccagtta gctctaactt ggcaaaaaat
    16321 cgggattaag ataagcttaa atatagttga tactgacaat attaacagcc aaattatccg
    16381 gcaaaaaaat tattccctgc tgctttacgg ccaaaacctc ggatcagaaa atgatttaac
    16441 cgcgttgtgg cattctggac aaagcggtgc taacggattc aatctgagta attatcgcaa
    16501 cgaaaaagtt gacaagagtc tagaaactat gcgatccagt ttagagctag ccgctagaca
    16561 acaagccgcc aaagaactac aaaatcaaat agttaatgac ttgccggcaa ttttcttata
    16621 taatgccagt catctgtata tccaacataa aaaaataaac ggctttgata ctgattttct
    16681 ggttgaaccg gctgaccgct ttaatgaaat caaccactgg tatataaaaa ctagctatcg
    16741 atggaaaaac aaaaattaaa taagcccaag tggcgaaatt ggtagacgcg caacgttcag
    16801 gtcgttgtgt tcgcaagaac atggaggttc aaatcctctc ttgggcacta aagattaact
    16861 cttttattac tcaaaagcca attgcttaat tattggcatt aattattatg attaccaagt
    16921 acagaactaa agactctgct gggcaagcgg ctagtggatc ttatggcgcg tctcgccgcc
    16981 caaaccagcg gacaaactat catcgttcca acactctcag tcaaaatcaa ggcttggaca
    17041 agctacgagt catcgtcttg ggcgggctag aagaagtcgg gcgtaacatg accatattag
    17101 aatatggacg agaaattatt attattgata tgggcctcca gttccctgaa gaagacatgt
    17161 tggggattga ctatattatc cccaataccg actatttaac cggcaaagaa gattggatta
    17221 agggagtagt tattactcat ggccactatg atcacatcgg cgccattgcc catttaatgg
    17281 gcaagctcaa aaacccgcca atgtttatgg gcaagttaac tgctggtctg gttgaaaaaa
    17341 aggttaaaga acatccgcaa tgcccccggc ttaaaattac tagaattgaa gaagactcca
    17401 aaattcaatt aggcaaaaat tttcgtttag agtttttacg agttaatcac tcgatcccag
    17461 attgttttgc ggcgattgtt cataccccaa tcggttcggt aatgcatacc ggcgacttta
    17521 aaattgatta ttcaccggtc aatgacaagc cggctgattt acagagaatc gcccaagtcg
    17581 gtagtcaggg tttattgtta ttaatggctg actcaactga ttcaactcat cctggttatc
    17641 aagtttccga gtcaactatc ggcgatgaaa taggccgctt atttgaaaaa attgatgggc
    17701 gaattattat tggcaccttt gcttctcaag ttaatcgtat tcaaaaaata ttctcattag
    17761 cagaaaaatt tggtcgcaaa atttatctac agggacgcag tatgaacgac aatgtggaga
    17821 ttgccaaaca aatcggctat atgaaatttg acgctaaaac cattattaat gatagtgaat
    17881 tgcgtcatat tcccgataat caggttatta ttttaggcac cggcgcccaa ggcgagaacg
    17941 gcgcctttct aatgaaagtg gttaataatg agcatcgggt agtcaaaatc aaacccggcg
    18001 ataccatcat cttttcgtca tcagttatcc ccggcaatga acgcagtatt caaagtctta
    18061 aagaccaaat cgttcgccaa ggcggtaaag ttatccacta cgaaatgatg gatgttcacg
    18121 ccggcggcca cgccaagcaa gaagacctca agctgatgat gcggctcctt aaaccaaaat
    18181 acttcatgcc gattgaagga aaccattata tgctgcaagc ccatgctgag ctagcccaac
    18241 aggttggtat tccttccaat aatatcttcg tcgccgacaa cggccaagtg gtcactttgc
    18301 agcgccaagg ccatgagatt gtcggacaac tcaccaaaga gaaagttaac accgattatg
    18361 taatggttga cggcttgggt gttggcgatg tttccgacat tgttctcaga gatcgcaaga
    18421 caatggctga ggacggtatg attgtagtga ttgccaccat tgattccaaa accggcgatc
    18481 caatcggcaa ccctgatatt atttctcgcg gttttattta tatgaaggat aataaagact
    18541 tgattcagga tacacgaatg cgtatcaaaa aaattgtcaa agaaactgat ttcttggcga
    18601 acccaatcag cggccacggc tttaacgatg atgatcaatt aaaaaataag attcgcgctg
    18661 atgtcagtca attcttattc aataaaacca aacgccgacc aatggtctta ccagttgtga
    18721 ttaaagttta aatttggact taaaataaaa aaacagcctc gttcgcgggg ctgtttttat
    18781 taacaaaaaa cgcccaaaac gggcgccttt gtttgtgcgg catcaacaat aaagctgaat
    18841 aaactaatta taacaaatag cctattggtg gtcaatagcc gccagctgtt ttcgacggcg
    18901 tgataaatct tcaaaacaaa gattaacact attttcgtcc tcggacacat tacccagcga
    18961 ccggaaacta ccgggaccaa aagttttttc cactagtttg ccgatggtta ataagctgcc
    19021 agtaaaatga gcccgtaaaa attcagtaaa aatagtatcg gcatcagcat attgatcgga
    19081 aaattcttgt tgtatttcct ggcaaatata ttttaataat ttacggtgct gattatatgt
    19141 ttccacaact tcaagacaat tttgctgaat attaaaccaa acaaaagaat cagaattaat
    19201 attattttgc cgcttaaaat tagcaatttg ttccttaact atgggtattt ccagaaatag
    19261 atgctcttca cggaaattgg gcagaagcat aattttatct aaagttctaa tctcctgttc
    19321 actaacaatc gcctcatgaa gacctttaaa atgaacatgt tttggcattt catgaccagt
    19381 cctggaatgg gctgaaactc ccgatcgaaa aagactaaaa tctttaacac cctgatcact
    19441 ctgcagggcg gtaaaagcct tgagatgcat agtctcatga aatataaccg ccgccaatga
    19501 taatttacta tccagaaact tatcattttg aataataatg ccttgtttaa gtttataggt
    19561 catagccgcc gctgtcatat ttaatctctg gcacgcttcc cgaggtaaaa aatgatagtt
    19621 ttcggccggc acactataac cctcaaggcc aaactgctga cataaacgat tagtttcgct
    19681 attagcaaaa gaaataaaat ctaacgcctc cggcgttttg gctagctcat atttcttaat
    19741 aatctctaga tctttatccg gtaatgaggc attatgctca aaaagcctgg cactaaaaaa
    19801 tctttagttt gctctttatc tttgggatca gcttgaccaa taacctgtaa attcggtatt
    19861 ctttccataa attaccgctc gtcaccactg ccgtgcaatt gaccgcgttg ttgacgagaa
    19921 aacaatttat ctagattatc ttgtgccacc tgattaaggt ccaaatctaa ttcagtggct
    19981 aattgagcta aataccataa aacatcccct agttctttgg ctactgcctg acgctgctcg
    20041 tcgccaacta ttccctgttg gtcacgaaaa atcttcttaa tcttttccac cacttcgcca
    20101 ctttcgccag ctagccccag agctggataa acaaagccct ggccaataac cggataaacc
    20161 gctgtcttat gactttgctg ttgatactct ttaaattcca taaatttatt ttaaaatagc
    20221 cgccgtgccg acagttatgc tagtaacaat aatcccggcc acgattacac cagcaaaaat
    20281 tggccaaaaa gcccgacgag ccggtacacc aaataaccaa gcagcaattg aaccggtcca
    20341 agcgccagtc atcggcagag gcacggctac aaacaaaaac agagccaata ggccatattg
    20401 agcaaattgc tgactagtct tacgccgagt ccgccgaaaa acagcctcaa taaccctggt
    20461 ccaaaactta gaataacgct cggctaattg aacaattttg ttaagcccta aaataatcac
    20521 cagaatcggc aagctattgc cgataaccgc caaaataaaa gcggtcagcg gtttaacatg
    20581 ccaaacagtg atagccaagg ggatggtcgc ccgcaattca gtaaccggaa tagctgaata
    20641 gacaatcgtc cacagctcct tagaagatag gatttcttga aaaataccca ttgatgttta
    20701 atcttattag ctcagttaac cagcaggctt agtttttaat aaacttatta tagaccaatt
    20761 ttaccaatat cgccagccaa aacaattggc cgcatcgcct caatctcgtc gcggtaatta
    20821 cattgaggaa tttgataaag taaataaatc ggtttattta aaacaaaagc aaagcccatt
    20881 tccaaaaatg aattgccacc aatatagcca ggaatatcct tacgctccac gttgaccacc
    20941 aaaatagcat caccagcctt aattttatca aaatgaatcc gaattaaatc atactgaacc
    21001 ttgttgcggg ccgattcggt atgagccttg tcaatcgtgg ccatggccgc ataatcgtgg
    21061 gtaaactccg gtaaaacaac ctcgtggccc aaggtcaaaa gctgtttttc agcttcaacc
    21121 atttccttgg aggccgacat actgccacaa ataataatct tcataaagtt atcattaaca
    21181 attatctaat taatagttta acacacggat gattaattaa aaaacccagc cggagctggg
    21241 ttataattaa ttgtttcgtt agattaaaaa agaacggtaa tgccgacggt tacaatagcg
    21301 accattgaca tcagtttgat taagatattc agtgctgggc cggatgtatc tttgcaagga
    21361 tcgccgacca tatcgccaat aacggcagct ttatgggcat cagagccttt accgccgaaa
    21421 tgaccttctt cgacatattt tttggcatta tcccaggctc ctccggcgtt agccagaaaa
    21481 accgccatgc caaatccagt cgtcatggca cttaaaagta agcccataac tccggcgaca
    21541 cctaataata ttccgatcat aataggtacg actatggcta gcaaggctgg accgatcatc
    21601 tccttttgag cacctctggt agcaatccga acacatgctt tatggtccgg attttctgag
    21661 ccatccagaa tccgcggatt gctaaattgc cggcgaatct cggcaaccat ttttttggct
    21721 accctaccaa cggcctcagt ggttaggcca acaaaaacca aaactaccat agcaccgata
    21781 aaggcaccaa cgataaattt tggattcatg acatgaatct catagtagtt gactaaatca
    21841 acgatattgg cctgggctaa aaaaagcttt tggccaccaa caatgacgaa atcacctgat
    21901 cgcaataggg caattttaat ttcctcaaga taggcggcta ataaagacat ggcggttagg
    21961 gctgccgaac caatggcgaa tcccttacca atggccgccg tggtatttcc aacagcatcc
    22021 agttggtcag tcctttctct aacttctttc ggtaatttag ccatctcggc attgccacca
    22081 gcattatcag caatagcccc aaagacatca gtggccaaaa cgaaccctat tattgacaac
    22141 atgccaactg cggccatagc gataccggaa agaccttggc taatgttaac gatatctagg
    22201 ctgaatccgt tggcaaaata gaaagctcca atcgttccta agcaaaccgc tgccaccgga
    22261 acactggtag atttcatacc taacgccaaa ccaccagtga caactgttgc cgaggacgtc
    22321 tccgcatttt cggcaatccg ctgagttgga cgatagccct gtgatgtctg atagttaacc
    22381 cagacgccaa taactatacc aactaacaag cccgaaacaa ccgccagaga taatcccaac
    22441 caattatgca aaccaagctg ccatgcaact acaaaagtgg cgacagcaat taagatcgag
    22501 cttaaccaaa tccctttatt aatggatccg actagctcct tcatgcctga attgtttttt
    22561 gctttgacag taaatataga aatgaccgag caaatagcgc ctatggcgcc aattatcatc
    22621 ggaaacaaga catatctgac ttgaagctcc ggggatagac catggctggc actagcaaat
    22681 atggcggcgg cggctaaaat gccaccggca tacgactcat aaagatcagc tcccatacct
    22741 gccacgtcac caacattatc accaacgttg tcggcaataa caccgggatt acgcgggtca
    22801 tcttcggtta aattaaattc atttttcccg accaagtcag cgcccatatc ggcggccttg
    22861 gtataaatac caccgccaac tctggcaaag agtgcctgtg ttgaggcacc catcccaaaa
    22921 aacaacatag tggttgttat gacaataagc ctggaagcag aatcaccggt atagaaatag
    22981 tttaaaaaac aaaaccatcc ggatatgtct atcagtgcca ggccgacaac caccatactc
    23041 atcaccgccc cacttcttag ggcaatttgc aaagcagcat ccaaagaagt tttagctgcc
    23101 tgagccactc gagacgaagc cagggtagaa gtgttcatgc caatatagcc ggaaagagca
    23161 gaaaaaaagc ctccggtaat aaaagcaaac ggaacaattt tactcattaa attaaagcca
    23221 aaagcggcaa cactaagtaa tatactaata acaagaaatg caactgcgac aaatttattt
    23281 tgttgccata aataagccat cgatccttgc cgaataagtc cggcaatttc ttgcattctg
    23341 ttatcacctg ggtgcttttt tttcatttca acgaaaaaaa accgtgctac taatagcacc
    23401 aaaagaccgg tgccgggaat aaaccagaag attgttggaa ccatttgttc ctcctttttt
    23461 atattgtgtt gtgaatgaac aacttaaaga attcttaaca tccttaacat atttaagaaa
    23521 aattagcaag ggtatttagc caaattatag ctaatttatc ctaatctttt ttaatttctg
    23581 ccgtcgcgcc aaataatcag gcataacctg ggcgactaat cgccagaaac gcactgaatg
    23641 attcatctct tttaaatggc acaactcatg gacaataata taatcagcta atccatcggg
    23701 taaatcaact aaacgatagt taaaattgag attgccttgt cgcgaacaac tgccccaacg
    23761 agatcgctga ttcttgatgc tgactcgacc atattgaaat ccgtaaaact ggttataata
    23821 ttccaatcgc tgacgaacca attgacgagc cggcccccta ttttgaagat aactcctatg
    23881 atcgctgttg attttagaat ctaacattga ctctacgatt aatcttttaa gcagcgcagg
    23941 gaatagccat cacgagggtc tggctggttt ctcatttttc ttccttatat aaattaactc
    24001 tgttcttcca cataatagca taaaacaagc acgacctaaa atcagaaaag ctaaaatatt
    24061 aaaacaagtt ttatgcggca ctcaaaacaa ccttggggct tatttgagat aaaaaataac
    24121 ccccaacact ttgatacaat ctcggtgttg aggggataga agtttttaac ggctaacgat
    24181 aaagttggtt tgccttagga aacatttcca tgtattcctt ggtaaccaca atatcataat
    24241 tcgctagcct gcgacgcata ttatcgctgc ttatgacttc atgccataga gccttagctc
    24301 gttcagacat gacaccttgg aacctgtcgg ccatataggc cctaaccaga tttttatgaa
    24361 agccgagcca tggagtaaag ttgtcctcaa aaacatcatt gacaagccgt tctagctcgc
    24421 caattccgcc acatcgctct gcttgatcta caacttctat ccaaagatca aagaagttca
    24481 ccagtggctc tttaactttc ttttgtttaa agaacctatt tcgtttaacc tcgttcaaga
    24541 aatcatagaa tatctttctg gaaattttag cggcctcttg attagccgag ttgaaagttc
    24601 cttcagaata gtcttcgatg tatggactat cagaaccgac accgcttacg gcgatgtcat
    24661 taacagtgat gacgagcttc attgtctctc ctttttaaag gttcattgtc tgctcttccc
    24721 ggcagagttg ggtaatgact aaaataaata taatcattat ctatataatt agcactttat
    24781 actgaatatg tcaatggata taatcataaa accaactaaa aacaaggcta ttcaccttgt
    24841 tttatctgtc tgcgggggaa gtgggacgta gttggaactt ttttagggta aaaactaaac
    24901 tttctttgat taattggcga tgttaaaccc gctatcttta gcataagaaa tattatagcc
    24961 aaaagctctg actacatctt ttatagcata atactgagcc tcagacatct ttagcgaatt
    25021 accctgacct ggccgcggcg aacaatatcc ttttatagcc tcagctaaag ctgaatcaat
    25081 cgattttaaa tcattaacga aagactcaac gtgttctgtt tctatcgatc caatttctga
    25141 cataaaaaaa ccatcttcaa tctttttacc caacaaatta tcgccgcgaa catatggctt
    25201 tatagtttgc gataattctt ctagcgcctc attgcttggt tgttccaaaa aataaagact
    25261 aatagaatca tgccgatcac ttggcgaggc tgatgtgcct ggttgcccaa atttatagtt
    25321 ggcttttatt ttgccgctga ttattagatt atcaatggcg ctgattaatt ctggtgtaac
    25381 attaacattc aacgatccac gagtaacggt ctcctctcca tttttaggga aaatacctcg
    25441 atacatccat tcactttttt tattgttaat ctctatattt ctactgttaa gtgcctcgtt
    25501 ccaggtctgc tcttcgtgag gatcaatacc ataatcagcc agccgatatt tgttataaat
    25561 atcttggaac aatcccttaa atagttgctg acgtgattct tgattattaa tgttagcaaa
    25621 aaaattctgg tacaaatcaa taactgcctg atttcttcct tggctaacat atttgattaa
    25681 cccagcaacc agggactgtt catcattgtg ctgactctca aataaatcaa tctccttttg
    25741 cttcatggcg attgtctcat cggatccggt accaatctct ttacggatag tttcaatttt
    25801 taagttatca attaatttat ttttttgtac ttctttctgg atgtccttat tatttctaag
    25861 actatcaacg gctctttgtt ttttctctct ttcttgttta gttctttcag gatcaacata
    25921 ctcaggaata gtccaaataa aattaagcgt ctcggcgtca agaccttggt attgcggtgg
    25981 tatttctgat ttgatttctt ccttgggctc gttttctttg ggcgaattta aaccagactc
    26041 aatgccagca ataaaagatg gtggcaggcc agacataatt gaatctttag attctccatt
    26101 tttaatacga gaaatatact cctgaatttt ttgttctgaa ccgctcaatt tttccaagcc
    26161 cataagcatt atttaaatca aatctttata ttataatact aacaaaaata accctgcttt
    26221 actagaatta tgccggcggg agatatggga cgtagttgga gcttttttaa ggtaaaagaa
    26281 aaccgctggg cgtaccatgc ggtttaatgt taaaaatcgt tttgagtttc accgtgtctc
    26341 ctttgggtaa gttattaccc cttgtacacc cgcgggcgtt attaactgcc ataatcgagt
    26401 gatttcttgc ttccctcctg aaccttgatc ttttgtttat gaagaagttc atgaggaagt
    26461 tcttggaact ctttgtcaaa ggttccattt tttcttccga aggctacctt ggtaactctc
    26521 cagtatccgg cgagatttcc atatagagag gtgatggcaa tatcctttat gccatcttgg
    26581 tcgaaatcct tgtagaccag ctcttctgga cgatctggta attccaagtt gaatctgcta
    26641 tcaaacggct cacttatgag ataataacga tcccaataag tagagccctg gtagtttctt
    26701 attccacttc cctgcacata cagggtaaat cccccatcaa ttttaagcca cgggagaagg
    26761 cgttttttct cacaacctgt ccctaatagc attgttgcta tgattgtgac taataaaaag
    26821 aactttttca tgactactcc tttttccgtt tttattgttt tgtattttta caaaaaaacg
    26881 gacttaatac tataacatct tatactattt actgtcaata tctacaacct gaaaaccaac
    26941 taaaacaagg ttattcaccc tgttttatct gtctgctggg gagatgggac gtagttggaa
    27001 cttttttaaa cgatttttgg ttcaataaat aacttaggaa agtctgtccc aatttcatca
    27061 acacccatcg ctattaactt atcacccaac tctttattgt taacatgata ggcataaacc
    27121 ttccgaccta aactatgagc ttttgccacg aattcagcgg taataatttc cgaaaatggt
    27181 gccaagaaat ctaacggtat ttggttaatc aaattccaaa tatccgccat aacctcttca
    27241 tctctaaata ataatccaac ccttagacct ggattaagtt ttttgatttc ggccagcaga
    27301 tttattttaa aagaggtgat aaatacctga tcttcaatgc cgttttgtgt aatcaagttg
    27361 ctaaccggct gtggtgtgcc ctcggccttc aattcaattt gtaaatcaat cttgtcctta
    27421 caaatattaa taacctcttg aagggtggga atagactcgt tacctaaaca acgcaacttt
    27481 tttaattcaa gtagattcat ttcaccaacc aaacctgtcc catcagtcaa tttggaaacc
    27541 tctttatcat gaataacaac tacttgtttg tcattggtca gcctaacatc taattcggtc
    27601 cgatcacagc caattttaat agcctcccta aaagaagtta atgtgttttc cggagcataa
    27661 ccagccgctc ctttgtgacc gacgattata gccataaata ttacttattc tgtatattcc
    27721 aaaaatactc tctccgtaaa accaccgcga gattcttttc gttcggcctt aactgccccg
    27781 atctcttcag aatcaaggcc aaaagctata attaaataat caatgacttc aaacacatca
    27841 ccaagctctt taatcaattc tgagcgatca gtattagcag caagaacttc tctggactct
    27901 tcaactaatt tatttaatag ctcattctta taatcatcct ggctcaaaat gctagttttg
    27961 aatttaacgc cccgcgcctc cattatctca ggaattctgt ctcggataag tttattgtat
    28021 gttttcataa cctaatctta gcaaaaataa ccctatttta ctaggattat ttggtctgcg
    28081 ggagaagtgg gacgtagttg gaacgattct aagataaaac accgagcgat tccaatcttg
    28141 gagccacgaa ggtcaaaata ttagaattga gaagacaggc taaaacaaat cggagtgaga
    28201 gccaattcta agcagtaaaa ccaatagctc ggctttttca atcttataaa taagcaaaac
    28261 atcaggtctt atgtggcact caaaacaacc ttggaactct ccaatcaaag gatgattgct
    28321 attcttctgg ggcaacactc caccaccgac taaaatatct aacactttct ctaattcaat
    28381 aaacagtcct tcttggtgtc gataagactt caaatctttc ttaaagcggc tagaatattt
    28441 caaagaaaac atagtctaac tgcgcaaagc atctttgatg agattggcac cgctggtaaa
    28501 agacttttta ctgcttttgg cgccaatgat tgattctctt aaaatctcag caccacgcaa
    28561 agttaaacca ttttcatctc taagttcaaa cgggatattc ttggcattga ttatctggcg
    28621 aaaaaagagc ctgaccgccg aactcatatc tatacccaaa ctatccaaga ctttcatggt
    28681 ctcttgcttg gttttaacat caatacgaat ttgaatttgt tcactatttt tagcaatcat
    28741 atgtttttat attatattca tattgtattg cgtttttaat aatttgtcaa tacaaataag
    28801 ctagctataa aagtagttgg aacttttttg gatgttaatc attaaaaacc aaccatttac
    28861 cttcactaat tatctctacg ctatcgtcaa ttacttttaa agctgaatta tcatcaaggg
    28921 cataaatctt atcagtttgt ttttgagcaa actctttgat taaatcgggg cgcacatgag
    28981 gaaaatgggg tgaattgaga tgaggaaaaa aataaaaatc caccaaattt aatcctagta
    29041 aatcctcagt gcgatccaaa ttttcctgat aaagtatttg caaactattt aaattaagat
    29101 ccttactaat aaccatacta ccagcactaa ctccgacata gaccttatta actaataatt
    29161 gtggcaataa tcttcccaag ccagacttat tgatccactc cattagataa aaattgtcac
    29221 ctccctcaaa ataaagaata tcggctgatt tcaatctagg ccgccaaata atcttatcaa
    29281 tagctgaaat atcagcaatt tcgatagact taaaattctg tttccgtaaa ttcgttaaat
    29341 catcaataag ccatccttta tcgccatttt ctatgtttga ggcggtagga ataaaaacca
    29401 aagacgtttc ctgtggcttt tttccaacta aatcaaatag tgccttagca attgattcat
    29461 tactaatacc agctgatgtt agaagtagtt tcataatata caagtgacaa aataatattt
    29521 tattatacca ccctgttcca aagcgataaa ctccctatca aaaataaccc tattttacta
    29581 ggattatttg gcctgcgggg gacatgggac gtagttggaa cagctttagg atgaaaatta
    29641 attaccctgt tcgaatcttc tcaatatttg taaataatcc tcctgcagtt caagggcaat
    29701 atgagaatca cctagaggag cgatctggcc attattatca ataatgttgg taatttcgtc
    29761 taaataaaaa ccgtccagtg tggcaatgaa caaataggat gggttccatg actggctcat
    29821 tctagtggcg gcctttaatg tatattctag acttagctgt cgttgatatt tcacctcaat
    29881 taattttact tgtctggttt ctcgatcaat aaccgcgaaa tctggagctg tccgtaatgt
    29941 ttcaatcatt ggattatttt cctgatagcc ctgttggatc atctcaggaa taactttctc
    30001 ataaccaaac tctaaaacag taaaattatt cgaacctcta aacatctgag aaaatattgt
    30061 ttcagcaatt ttccccttaa ccaattgccg ggtaaaatca agattactca tattatttat
    30121 attaataaat tattaaaaat catcatcata ttcttcatca tcgtcatcaa aaatattctc
    30181 aatttcaccc tgtacctgtt cgttattttt ataacgaaca ggcggcggag catctattat
    30241 ctcattaatt tttttaatat gctctttacg tatattctta tcaacaatat taatttgatt
    30301 aatctcatca acaattctat cccgatcgat gttagataaa acaaattgat tatgaaattt
    30361 aattttctta actacttctc ctgcataccc aaccaaatca gtccctgtaa tcctcagatt
    30421 atccgcatca ggaaaggcca cataagaata aactggtacc ttaatattta atgttgcaat
    30481 taagttttca atagttttta catgaccaaa attttgccac aaaggattat agaaacggct
    30541 tttcttatga taaataactt gggtccaata tttatcctcc gctcgaccaa atatccagcc
    30601 agaataagct ttagtttcaa tacaaaatat accaaaatta gacacgacta tttgatcaat
    30661 ttgagtggtg ccaatttgat taccagcgga tggcaataaa acatcactta acactttata
    30721 gtaatcatgg ttcagcttag ataatttttt gttaacgaaa cactcggtat tcttaccaat
    30781 aaattgcggg gagtgcttca atcctaacac tattgataaa acaaatgcta cgaaagatcc
    30841 catattatat tgataatcta aagtactata aattttatat aacttataaa gtattatatc
    30901 actaacataa aataaaaaca caccatttca aggtgcgttt ttgcgttcgc ggagagggtg
    30961 ggacgcagtt ggaacggtta tttctccaac atcttcctta actcgaaatt ataaaggcaa
    31021 aagccgtgat tatagctgct aaaactaaga acaaaataac atattttgct atttttaaag
    31081 attttgtttc tttcacccat actgagtcag aagaaacaac attcattttt tgaagaagga
    31141 ggccagctaa agacgcccca actaaagctc ctataatacc aaagtataga ccaattaaaa
    31201 ctaacattac tgataaaata atggctaaaa ttttttttat agttttcttt ttttccatat
    31261 caagtcaagt taattaacaa aaaattattc aaagcttata ttaaatgccc cctctttatc
    31321 atttttttcc ctaaactctt ttaaaaatct attaacattg ttaactgata ttactatccc
    31381 cagattctcg taattcccaa ccgtaacggc cgaggggacg ccaactatac aaccattttg
    31441 gtctactgct agtccgccgg aattaccgct atctattttc gctgaagtta ttatatcttc
    31501 gttgtcgtca aaactactaa tcagcccatc tgtaatggtt agcgctttcc cgccgctaac
    31561 cgaaggaaat ccaaaaattc taattttctc ccctaaaatt gggtcgccct tgtttacaca
    31621 ggctgaatta tcgtagattg caaaatcctt ttcataatca ccatacgtat agccatcttc
    31681 gtctgtataa gaagaaatta tatctaaaaa tgcaagatcg tattttttgc ttaaatctaa
    31741 aaataccatg ggcttagcaa tgtaagcttc agccggtgag cctagttttg gctctgataa
    31801 tacaaccata catcttatat tatccaaatc accaggcttc gcgtatggca ttatgtgaaa
    31861 attagtcaac acaactccag ttgaggaaag catggtgccg gatccacctg tttcagcttt
    31921 atcaaaatat ttatcatcct tagtggaaca aataatatta acaacggatt tttttaagtt
    31981 ctcttcaaaa taattttcta attctgcttt ctcagttgtc tgattaacag tttgtttctc
    32041 tgtattatta gatggttcag agtttaacgt ggaaaaaatt ataattaata taaccgcaaa
    32101 gacagcaacc aacatccact tatatttttt tattttagaa aaaaaattgt tttttctact
    32161 gctaatacta gcaccacatt ttttacaaaa attagcattc tctgataatt caaaattaca
    32221 tttgtggcaa cgcatattat ttcatctttt cgccgcagtt ctgacaaaaa ttatttttaa
    32281 tactattttt ataaccacac tctgaacaaa aaatctggct aatgctgtcg tcattattat
    32341 ctgtgatttt ctgttctacc tccccctcaa tatatccggc gacattttcc tcaacttttt
    32401 tgggaccagc caaacgctgg ttatattctt ctatggtaat tcttttatca atcaaatcgg
    32461 aatttgcttc tgctaaagtt tttaattctt caaatttatc actatccaga tatatacctc
    32521 tttcacatac cgggcacatg aaaaaatatt tacgttcata tggtatgacg ggaataaaaa
    32581 ataaggtaaa ccatgtcgtt attcttaata attgccagta gtcttcatta ttgcaactgg
    32641 aacaacgatt tttgaatgtg attccgtagt tttttgttat ttgatgaccc caaccaaata
    32701 tgaaaaacat actctaatct ataaatattt tatcaaaatt atcaccaaca tagaatcttt
    32761 tttgatcatc tacaaactca ttaaagaatt ctgaaaaaat ttctttacta taatggcctg
    32821 tgtatatcgc ttcgaaagat atctgggctt tcttatcatc atcaagctta ataaaaactt
    32881 tgcaaacatt tagagattta tcatttgcca cattggcata tttaagcatt tcattcgttt
    32941 ccttcttctt agtagttaaa ttaactctaa ataatattaa attatctaat attttataaa
    33001 atacaatatt atttaacttt ggatgaatgc atgaataaat atatttgctt ttattttgct
    33061 cgcccgacat ttcttctttt gatattttat atccaagaaa ttcaagatga ttatttattt
    33121 cttcgcacac agatttatca cttttcgctt cttctttttt aggaataaca actttttctc
    33181 cgcatttagg acaaaatttt gaattttctg gcaaatcaaa gccgcatttt aaacaatcca
    33241 taaaatttaa attatttgaa ataatattta gtcattagaa ttcacaaaat ctttaatctc
    33301 ttgaggaact tcagctcccc catccatcgc ttttttgaga taaatttttg cattatccat
    33361 atcttctttt ttcacataag ctaatcctaa ccaataagca gtatatggat ttttatttaa
    33421 atctgatgag gataatagta aaattgtttt atcaaaatta tcattaaaat aatttgcaat
    33481 cgctaaattt tgtttagacg tttctgatcc gtcaagatca taagccttct gcgcataaat
    33541 aagggcttta gaataatctg aagactgcgg agaaatttct tccatatcta aataaaataa
    33601 agccaaagca ttattaatct gaaaatcatc cgggttcatt ttataagcgg tgttgtaatt
    33661 tgctaacgct gcattcggct ttttctcaag taaatttatt tctccggaga ttagataata
    33721 atcaaaggac tctggagttg ctattgcaag agcctcctta aaagaattta atgctaattc
    33781 attttgccct tccgttgaat atacgtatgc taaatttttt aaagtattaa ttttagtact
    33841 atcagtaata gctccttggg aagcttcttg gaattgtgtt atggcttgct ggctatcgcc
    33901 ttcatgaaaa tttttcagag cttgattgtt ggtatcaata acatcactat ctaacgaatt
    33961 ataaatttta aatatcaaaa aaccaccaat taacaacact cccagggaaa ttttaataaa
    34021 cttaactaga ttatctttat agttcttttt cccctttgtt ttataattgc ttattcccgg
    34081 atcgtttaca atattattaa tactggctcc gcagcttgaa caaaattttt tattaccgtc
    34141 tatttgtttg ccgcagtttt tgcaaaacat attattatga taaaagagaa atcaaaataa
    34201 gaattaaaat gagcgcccca aatatagacc ccattatgat ccaaaaattc cgccaaaact
    34261 ttttatcttt tttctttttt tcttctgaag acaggttact tggaaggcta attggaggaa
    34321 cttttttgcc aaggataatt tttaatgggt cggtaatatc tggataaaac caaaaccagc
    34381 taattggcgc gaaaattatg ctaaaaacag cgctcgcttt ggaaattttt tccgctttta
    34441 ttacttttac acaataccaa acaaaatata ttagtaaggc tatatcagcc aaccaaagaa
    34501 tggcatataa tccttcatct atactatccg cccctcctct aacaattact attccaataa
    34561 aaacggctat aaataaaaaa tttaaacgat gtaaatattc ctttgcctct tcttcgctct
    34621 taatattagt gtgagccata tactcttcac gagtcatggg cttattatca cttttactat
    34681 tttgaagagg tgctttttta tcctcattgc cgtcagatat tttacttcca caattatggc
    34741 aaaattgggt gttttctatt attttatttc cgcaattttc acaaaacata gctttatttg
    34801 tttaatttta aatatttaaa taaaaaaata attaaaatta tatcgaccac gatccatgcc
    34861 ggcctaaaaa gtaaatgaat tggtataata ggattatata aaattgctat acctaaaaaa
    34921 taccaaaaaa atttattttg tttttctaaa ttttttttgt aattttgaga acaataataa
    34981 acagcaacgc aacacacaat taatttaccg aaataataaa aaccgtattc caatggaagg
    35041 atggttaaaa ttaaagttaa aattgcaagt tttattggcc aagtttttaa catgaatcct
    35101 ttttgtgagc agaacttaat atatttaata taatttaaaa ttaccacaaa acgcctaaaa
    35161 atcaacaaaa ttaggaaatt gctattagcc ctcttttatg ccaaaataag actatgactc
    35221 ttcacgaaat caaacaaaaa attgagattc tgctcgataa atatcagaaa aattcaggca
    35281 ctattaaggg ctttgacatt ctttttgaat atgttgattt cttaaagagt gaaccataca
    35341 cagccaatct gttaaagcct caatttgatt atgccaaaga ccaaatagcc caactggatg
    35401 aatatatagc taagaatagc tgtttcaaag aggtgcctaa tgttcataag ataatggagt
    35461 ttaaggatgt ttccgaggct cctatgcata aagatgtaat agctgtcggc ctggaacaat
    35521 taaagagcca ggaacacgct aatatgtcgc aaatgctacc cttagccatt actcaactag
    35581 ttatgatgta cgaggggatg ggtcgggtca aagaacaatt aaagaataaa gaagaaatag
    35641 aggataaatt aaatattctc caggaactgc caacatctac attaagagtt aaagtcggcg
    35701 aagaattaaa cagcctgcca ttttctgttt ttgctaaaac aaacttgctc atttccgcca
    35761 aatacatctt ggataaaatt gatattgaag agctatccga agaaaccaaa aaagagccct
    35821 ggtttgataa ggataatagc gttctatatt ttcaaaacaa gcagataaaa ataactctca
    35881 aaaataataa gcctctggat cattatatcc ttgaggcaat attttctaaa aacgatttaa
    35941 acgaacagac tgacttcgta gaaatatccg aagatattat gaaagaagaa tataacggca
    36001 aatgggaaaa attccgtcat gcttgcgaca aactgaatca gaaaatattt aaagcgactg
    36061 acaatactgt tagcgacttt atagaatata cgacgggaaa gacgggttgg tgcaaagtta
    36121 atcaaaaata ccaataaaaa caattaaata gactttaggc cttgcatgca aaatggtgca
    36181 aggccttttt tgtatgctta agtcatgatt caacattatg accaacaact gattttagaa
    36241 gcccaagaat actttagtgg attatacggg cgagaaataa ctcctgatga agcggaatct
    36301 tttttgaaat ctttagttaa tttatacgat tctctttcta ataaggaata actctttttt
    36361 tcttttcggc ggttcccccg tagggggagc cgccgaaaag aaaaaagtta tccttgttaa
    36421 aagaacttct aattagaaat agataataac aaatatatga aacagcgaga taataaatac
    36481 ttatctaacc gtaaactagt aaaatctggt tcgcttttag agatatatca attcggccgt
    36541 gatattaatt tgggcagaaa gataagacat attaaaaccg aagaagaaaa ggccttgcag
    36601 aatggcaggc cgaaagtaag caccgaagaa aagttaaagt cttctgctcg tcgggcgaga
    36661 agaatgatta aacggctgat tatggctaat tgttttctgt ggtttaaaga taagggcaaa
    36721 ccatatttgc caattacttt aactctaact tttgccaaga atattactaa tctgaaaaag
    36781 gctaattatg aatttacgaa atttatcaga cgacttaact acgaaactaa cgccattgag
    36841 ggcaaagact taaaacaaag taatttaaaa tatctagcag tgtttgaact gcagaaaaga
    36901 aaagcaatcc actatcatat gattttcttt aacctgcctt atatcaaaga tgtttatcgg
    36961 aagatgtata agatatgggg tcaaggacgg ataatggtcg gcggaaaaga taggaacttt
    37021 aataaagtta aagatcaggc tcaactgaaa aagattatct tttactttat caaatacatc
    37081 caaaaatcgg tctttgaaaa tgacttccct cggcaaaaga aatatattgc tagtaagggg
    37141 ctactcaaac cgcaagaaaa ctcgttcgtt gaagtcgtgt cgcttgtgga atccaaatta
    37201 tcagataaac aactggtcta taagtatgac ggtgaaatgg aatataacaa tagcgatatt
    37261 agggatacgg tcgggcaacc gcaaaatttt ctccgctggt ttaattacca gcaatatgat
    37321 ttgtcggata atcctgattt agataaagaa ataagccagc tattagctgg cttatccata
    37381 gacgatattg atttttagac tgttctttag tatattaata atattgacct aacataatag
    37441 acaaacactc ccagggtatt tttttaacaa aatacctatt tgtcgctcga aatgggcagt
    37501 tctgccaaac gataaattaa cctttgggct agtttgttat gttgggtcaa tcaggactgc
    37561 cctttttgaa tacaaaaaaa acaagcacca ctctttgtac ttgtttttaa aatattgatt
    37621 atttattttt tcttaaatgg tgccatcaaa aagtttccta atatttctaa aactggcaaa
    37681 acttttaata taaacctaat aataaaaata gcaattgcaa atttccaatt tataaaggct
    37741 aaggcgataa ttataatcca gtcaatccaa aaaatatacg caacccaatt aggcaacatg
    37801 ggttcatttg ccggcaaagt gccattcttt ctcccttgag tcattcggat atcaaatgtc
    37861 gttatagatg atgtgattag ataaccgatt gtaagcacta gtaccacagg agagcttaat
    37921 ccttctttta aaaaatccag cataaaatta ttacttaata aatatatcta tattatagct
    37981 aaaacttaat aatatgacta caaacgacca aacaattaaa tatgctcttt actgccgaaa
    38041 gtctaccgag gacagcagtc gacaaattct ttctctagat tcacaagaga aagaaatgat
    38101 aaaactagct gagggtttag acttaaccgt gattaaaact ttccgagaaa gcaaatccgc
    38161 caagaagcct gataaccgtc cgcaattcac cgagcttatc aatttgatca agcgaggaca
    38221 aattgacggg attatctgtt ggaaaattga taggctttct cgcaatccga tagactcggc
    38281 tactattcaa tggttattac agcaaaacca attaaagatt atccaaacta tggaacggca
    38341 atatctgcct agcgataatg ctctcttgtt taatgtagaa agtggaatgg ctaatcagta
    38401 tattttggat ttaagtaaaa atgttaaacg aggtattaaa gctaaactag aaaaaggcgg
    38461 atggcctaat ttagctccga tcggttatct aaataaagac agtaaaatta ttatcgataa
    38521 agcgagagga aaatatatta ttaaaatatt tgaattatat cggactggta aatacagcgt
    38581 taaggaaatt gccgaaatat tatttaaaga aggattcaga agtcgagcag gcaaaaaata
    38641 tcataagagc aaaattcata agatgttatc ggaatctttt tattacggaa tcatgactaa
    38701 agacggtcag ttatacgagg gcaagcatga gccgttaatc tctaaacaat tatttgatga
    38761 ggttcaagaa atgcttaatc aaaaagctca tatcaaaaaa caaaatatcc cctttgcttt
    38821 tagggggatt ctaagatgtg ccaagtgtgg ctgtctgcta actgccacca agaaaaaagg
    38881 gcggctaaca tattactatt gcaccaacgg caagaatatc tgcgaggagc ataaggccta
    38941 tatcaaagaa gaatatttat ctaaagaatt aaacactatt tttgataaac tagtttttaa
    39001 tgaaaaaata gttaatctag tctacaaggc taagttaaaa gaaagcacat cggccatatc
    39061 ttatttagac gaggttattt taaacctgga taatcgagcc aaaacgctca atgaacgcaa
    39121 aaaagtatta cttgatagct acctggataa aatcgtttta gagggcgatt atagggctaa
    39181 gagccaagcg atagagaacg agctagtcct tattactaag gatgtggaag agttaaaaat
    39241 caaaaaacta aaccaggaca atactacttt ggaacggata aaaaacttct ttttagcccc
    39301 aaaacagatg aaaaaagagt ttttacgctc tttacctgaa aaacagcgag atacgctaat
    39361 cggcctactt tggaacgccg aagttgaaaa caaaaaaatc gctaatattt catttaaaca
    39421 accctatgat gcgatgtcta aaattgaaaa taaaagcgat ttttcttcag tgcggaggag
    39481 aagggattcg aacccttgat gccattgctg acatagtgct tttcgagagc acccgattca
    39541 accactctcg cactcctcca agtggtttaa acccttctat cttgggctaa atgaaataat
    39601 ctgtcaatct caatatcagt taatggtcga caagcaccaa ctggcaagcg acccaatttt
    39661 aatgagccta gagcaactcg cttcaaatca gtcacctcat aaccagcctc tttcatcaat
    39721 aaacgaatct gacgtttttg gccttgccgc agaatgattt ccaaacgatc cgtggctaat
    39781 tgctggccga cttcagctgt agccaggccc tggtcatcat aaagacggcg acctttagct
    39841 aaaaaatcct ttagcttagg tagatcacga ccaattaaac cgggccgggt agttgcctca
    39901 tagaccttag catgagcgaa gcgcgggtga gtcagtctct cagttaactc accatcgtta
    39961 gtcaaaagca ctagaccgcg actgtcttta tctaaacgac caaccacaaa taaacgtcca
    40021 gggtaatcaa ctaattcaaa aacattatgt tcatcacgaa aatcacggct ggtgcaagta
    40081 tagccagctg gcttattaag cataaaatag accattggct cgtcttgact agcaaccaac
    40141 cgaccatcaa cttgaacagt ttggttatca ccactaacaa cctgccctaa ttgagctatt
    40201 tgattatcca ccttaactcg accagcccga atcaaagcct cggcttgacg acgcgaccac
    40261 ggaccgtggc tagctaaata tttagttaat tgttgattaa tcattttgtt ctttattatc
    40321 cgttagttgc cattttaacc aaattgttag ggctaaaccc aaccagaata aaaccgctaa
    40381 atcattttta aaataaggca catccaccaa gccatgaatg gcggcggcta accaagcccc
    40441 taataatccc caactcaacc aggctaattt ctgacggcga tagcgccaaa aattaattaa
    40501 gctgatcagc gccgctttca gccaaagcca aacaaagata atcaatccaa ctaaacccaa
    40561 ctcgctccaa aaattcaaga aaatattatg gggatataga taaatttcca gtggctgcca
    40621 gcttggttta gtttttttat cagctgattt tatccaaatg ccttcgtgat gatagggttc
    40681 tactgttgct tgataattag ctagcccggc cccggtcagc caacggccat cactaagcat
    40741 ctgccaagtt tcctgccatt gcgactggcg aatttgaccg gacaagtcag ccaaccgcac
    40801 tttctgttca atcgttgcct gataatccgg ccgctgccca attattattc cggcgacaac
    40861 tgtcaccacc aaaaccacca accgactcca ccgattagtg agcaagccca aaacaaccaa
    40921 ggccgcggcc aaaccgatca ttgccccctc actatgcgcc caataaatgg ccagccagcc
    40981 caaaataata ctgctaatta gtaataagtt agaccaatta agccagccct caactttttt
    41041 caagcgataa attaaataac cgagtaaagt caaactaact ggcccagcaa ataagccaat
    41101 agcgttagga tagccaaaaa ccgaggtcgc ccgtctagta gcggccgccg cccaaaaaga
    41161 attggcaatt aaattaccgg tccaatactg atagccggct atcaagccaa ccaccaataa
    41221 actgccggct aaatataaat agccacaatc aatatatttt gttccacgcg gaacattcca
    41281 aaagagaata aagactaaga ttggctcaat aaaataggcc ttccataggc ccaaagcgct
    41341 ggcggtatta ccgccaatca gtaaagccag ccaagcgatt aataagacta aagtgatctc
    41401 aatagtcagc ggataggctc gccaattctt agcccggaag cgcagattgg cccatggctg
    41461 cggatattgt ttaatcaacc aaatggcagt ggttaagaga atcaattctt ccaaaatagt
    41521 catcggcagg ggcccaacct tgaaacgcaa tagataggtt ggcaataaac cagctagcaa
    41581 gctaacagcc aacggccaac gacgccaagc caatatagac caaaacacac cgaccaataa
    41641 agcaatcaac cacatattat ttatccaatt gctgtaaatt ttttaatacc caagtattat
    41701 ttagcggatc atagagggca aaagtggccg gataataaac tccgcctaag gttcccgggt
    41761 taacgcgcca aatattatcg ctttgctctt gccacggctt atgggtgtgg ccataaaaca
    41821 aaacatcaac cggaccgtca ttaagaacaa tatcctgata ttccggttca tgacaaactc
    41881 caatagtgcg attattaatt acaaaacgac catgacggcc caaattatag aagttaggcc
    41941 gcgagggaat atcggctgga gcgtaattat cggcattgcc ttgaacaaaa tagagctggc
    42001 ctggccacag agatgccaac tgctgaaacg tgtcactatc ggtcaaatct ccacaaaaaa
    42061 ccgcctcttg gatcccctca gactgacacc attgagccca cagctgcaaa ttaattaaat
    42121 tgtcgtgtag gtcgctaaca accgccaaca tattaattag ccacgcgcca aatttcgtct
    42181 aggctagttt cgccgttggc cgccttcaat aggccgtcgt gggtcatcaa tagcgccccc
    42241 tggctaatag ctaattgttc tatctcggca tcagtagtta gcggctgttg gattaatttt
    42301 tgcaattccg gggtcataac gatcacttca taaataccaa ctcgtccttt ataaccaatg
    42361 ccgcgacact ctggacagcc aaccggacca taaaattgcg gctgatcagg caactttaat
    42421 tcgggacgag tttttaataa ttgccaaaat ggctcaattt gggtttgcat ttccggggag
    42481 atgttgaccg cttgtttgca tttggaacac agtcgtcgcg ctaggcgctg gccaatggaa
    42541 cattccaggg cgctagctag catggttcgc tctacaccca gtccagcaaa acgagcaata
    42601 gcgccagcag cgctgttagc atgaatagtt gacaggacta gatgaccggt caaactggcc
    42661 tcaatggccg tcttggcggt ttcatcatcg cgaatttcac cgaccataat aatattaggg
    42721 ttctgccgca tcaatgaccg taaagcagca gcaaaagtat agccgccagc cgtatcaatt
    42781 tgagtttgca ttaagccgtc taaatgatat tcaatcggat cttcaatagt aataatcttg
    42841 atttccggac tattgaggcg ctttaataac gcatacaaag ttgttgtctt gccggaaccg
    42901 gtcggaccgg tggtaataat aatacctttg gttttatgca ttgatttttc aatggccgcc
    42961 aaagaagcat cgcgaatgcc caaatcagcc agctctaaac taatggcctg gttagatagc
    43021 aatctaataa ccactgtttc accataaccg ccggtaatca aggaaacacg gcaatccatc
    43081 cgttcggccg gtaagtccat actgaaacga ccgtcaaaag tcggctgttt aacgcttaat
    43141 gataaaccga ttagattctt aatttcacca attaacggaa cataatgctc cgaacctagc
    43201 gtaacaatat cgtgtaagat accatctagg cgataacgaa tcttaacccc accttcaatt
    43261 ggatcaatgt gaacatcggc cgctttacta acaatagcgc cagccatgac tgaagccaac
    43321 atttcgctgg ttggtttatt ggccaataat ttttctgctc caacaatatc ttgagctacg
    43381 ctttcgccaa actttagatt gtcttcggaa atttttaaac cacgacccaa ggtttgcgaa
    43441 acgacaatat cataaagctc atcaatatag tcagtcttac caatcactct cagcacttcg
    43501 tcaaatgacg tttggccatc taaaactttt aagataccgt cttgtagcat cgtcaccatg
    43561 ccgtcttcaa tagcttggcg cataatcttg aacgatggca ctccatcagc aatcaactct
    43621 ttcatcgctt cactcatggt aaaaatttcg taaataccaa ttcgaccctt ataaccgaaa
    43681 ccggaacaag ctggacagcc gtcgccggcc tcataaatag ttggcaattc tttgggcaca
    43741 tcaatattag ccttgggtga aataactgcc aaaatcttat ttatctgttg tctctcagct
    43801 tcattgggtt gatgaacacg gcggcactgc ggacaaagct tgcgcaccag acgctggccc
    43861 ataatcgcat taatcgctgg aactagttgg tagggctgga cccctaaatc aactaggcgc
    43921 ggcaacaccc cggcggcatc gttggtgtgc agagtggaaa aaaccaaatg gccggtcaaa
    43981 ctggcttgga cggcaatttc ggccgtgtct aaatcacgaa cttcgccaat cataacgata
    44041 ttgggatctt gacgtaaaat tgattttaaa ccattagcaa aagtatagcc gcttttttca
    44101 tcaacctgac tctgattaat accctgaagc tcatattcaa tcgggtcctc tagggtaatg
    44161 atcttaaccc cgggctgatt aagcttgtta aggacagcat acagcgtagt ggttttaccg
    44221 gaaccagttg gaccggtggt caaaaccaag ccattgggct tggccatctc attctccaaa
    44281 atctgccgca cctccggccg catccccaga ctatctaatg gtaaaaccgc tgatttcggt
    44341 cgcaataagc gcatcaccac actctcgcca taagcggttg gcaagaatga actgcgcacg
    44401 gccacttttt catcaggcaa gaaaatatca aaacggccat cctgcggctt gtcctcaaca
    44461 ttgagcttaa ctcgggcgac aaccttgagc cgagataaaa cctttttcca ttgctgacga
    44521 ggaatggtgg ccacctgctg caacaagccg tcaacccgta aacggacaat aaccgctgtt
    44581 tcttcggcct caatatggat gtcactactc tcagctcgca aagccgcggc aatcaccaat
    44641 gtcaatattt ggctaatatt ggcttctttc aatttattat tcaatagact catctcacta
    44701 agctcggcct gatagcgagc caattcttcc gtatcaatct ccaccccctc taaacgagta
    44761 atcttcggta aagtgtcata aatcttatgg gcatattcta aactgcgttg agaaatcaga
    44821 catagcttaa tcttaagacg attattagcc gataattctg ctattttggc cgttagggct
    44881 tcgctgggtt gagttgttcc aattcgaaca atattcttct cgtaataaaa acagacggct
    44941 gctaaatcag tggcatcatt tctgtctagt aggctaatcg cctccggact aatcggaaaa
    45001 ttcaatagat taatatatgg cagtccctgg gcctgagcct gagctgctac ctgatcttca
    45061 attgcctgat agcgaagatc tgattgccgt tgactaagct gagccgctgg cgaatcttta
    45121 ctggccaacg ccttgtcatt aagcaaatct tcaatggaaa gatgattgtt ttcaaccata
    45181 aatttaattt atttggctct tgcgctcttt aacataatta gtccaatcct cggccatatc
    45241 aataaaaaat ttaaatgggg tttcaataat aaaatctaat aagaacacaa aaatattaac
    45301 tttgctaaat ttctcactca accatttacc aacagccatt accggtaaag taaaaatatc
    45361 aaacagaatc cgccacaagc tctctttttc ttcagtaatc tttaattcat tggtcacccg
    45421 cttgatgcga ataataaaga agctgacaaa gactaagaaa aataagaaaa taattatgcc
    45481 aattaaggta aagtgcagaa tatgtagcaa ccaaacaata gcgctgaaag tcactaaaaa
    45541 agtcagggcg taaataatat taaaaaattt cgttaatata ccggcctttt tttggggtaa
    45601 gcgaacaatg attggtgttt cacggcgatt ttcctcaaaa gataattcat taacgatgct
    45661 gattatttta tttgtattag attcattggg cagtttagta atcaataccg agaagaatag
    45721 cagcgctgcc gggaaaagag cattaatcaa taaagccatt acactaactg acgcgccaaa
    45781 tattttattg gctggaattt ccagagcaat tactaaaatt gttttggtta aaaagatata
    45841 aataatacta cgcaccgctg cccgccacaa taaattttta gctttagcat agttcttttc
    45901 acaaactttt ttaatctgcc gcggaaaagc tttgggatca gtcttgatac tcgtataaat
    45961 accgaccgga tcagattgta aaacatcgag caagactgag gtgcaaaccg cataacgacg
    46021 agtcaattta tcgagcgctg gcgctaaggg gtgctgtaat tgctgattga tagcttgacg
    46081 aatttcatta atttttttgg caacggctgg ccaacggctg gcctccagtt tctgccaatt
    46141 agctaaataa tatttaaata aaataaaagc cagcatatca ttgtctagct tggcaaaatt
    46201 acgattgacc gacaggtaaa tttgaattgg taagtcagct gataattcgg gatgattagc
    46261 tagctcaata ttatctagta aatattgata taaattatta atggcttggt ttagcgcctg
    46321 atcattaccg agccggctct ctatttcgct ggcggccacg gccagcaacc aagctaccaa
    46381 gtcgttgcgc tgcgacaatt cttctttgaa tttgactaaa tccttcttaa ttaaactggt
    46441 cggctcggcc tgatggtcga gttgagccac ggcaatgtct ttgataacta agtatttcct
    46501 aataataacg gccacctcgt caattaaact ggtcggtaaa taattgttgg gcaaataacc
    46561 accacggatc aattcggcca atagatgctg gcttattttt tcatgatcat tggccgctat
    46621 tgtgctctca atcaatatct gccgccgcaa aatccgctca atcgcttctt tgcgcaataa
    46681 atattcttct ttataatcaa cagcattccg aatcttttca taaaaaaaag acatccgcga
    46741 aatgacgctg gaaaccttaa ttctgggctg atcacccttg gcggcaccag aactatgctg
    46801 agcgcgataa atagcgcgca acaatttctt ggtactatca ttaactgtca aggtcacaac
    46861 cgaacgtttg tgaatttttg gatttaacat aaattaacca ttgcttcaat taagcatagc
    46921 caaaaaaacc ctaaaagtaa atgttaaaaa ccaattattg cccttggtct gcttttatct
    46981 cgtcgtatat ttcaccgaca aaatcggcta ttttcggctc gacttgtttc caaaaatcca
    47041 gataaccact tggatagtgg gcatgttctg ctaaatattt gtcccgatcg cgataagacg
    47101 gatggtctaa aaattcatcc acattgtatt tatcaaagaa ttcttgagtt ccaggaatag
    47161 tgtccttaaa tcttaattga gaaattagct ccttataatg agctccggaa ttatcttcat
    47221 taatcgcata atactctgtt tttaacagtg gtttattttt aaaatccgga ccagtaaaca
    47281 tgtctgttcg atcataataa agtattttag atggtctggt ttgcggattg gcattaaaat
    47341 aattctccca gtactcttta ctagaaatag tgttgtcggc cgacggcctt tccaaaaata
    47401 atctaaaaat gtttttgtcg ggaaaacgtt gatatatttc actatttaga taaaaactta
    47461 aaagctggtc agcgggttca ttggaatcaa acctaaaagc agtaacaaaa tctttaaaca
    47521 aagttgtttt attggcttta aaataggcca tcgctttttc tccatgtccg cccggatgtt
    47581 ggggagaaaa caggttgctg ctcgagaacg aatatggtaa acatccatag gcatattccc
    47641 tatcatcacg aataagctgt tcctctaatt taataaatcc aaacttccgg gctagttcaa
    47701 taacctctgg cctatcaaag aattctatat ctcttgatgg aagagaaggt ccagattctt
    47761 tccatgacca gtaatcgttt cttatcttag ttagcgctga attaaattct aacctggcct
    47821 ggttaatctc ggtcaattga tctctgcatt cggaacagac cgttggctgg cgatttttat
    47881 ctaaaacata tttagacccg ctagctttat caacaggcac attttccgga atacaaacaa
    47941 cgcccagatc caatgggata ccttcaccag cactccaggc cacctgatcg ttgtgttgat
    48001 ctttttgcga agatcttggg tgttctgggt ttaaattgtg ctgaaaattt ttagctataa
    48061 cctcaactgg aaaataaaag tatatagtat aacctgcttc agctccataa tcttctaaaa
    48121 tatggttata accaaaatgc gtggctgagc agtctggttt tggacaagcc gcaaaaagtt
    48181 cccggttaaa aaaatcatgc ataaacattt taacagcctc gtctttcggt aaatcaatcg
    48241 gtcttttggt ccgccagtat gacgataaaa gatcctcgcg gatatatatt ttagcgtaat
    48301 cggccttgga aagaatatga tcaaccaacg atccaagccg gccagactct agtattggct
    48361 taaatccatc gatcacttgt ccctgcccac cgcaatgaac accaaccgtt tcacgtaacc
    48421 caaaacgagt cacatgagaa aaatagagcc cggagagcct gttagccaat gaaagatagt
    48481 cagccaaatt catcttagca atattatccg ctgataaaag ccgatcttgc tcgtctttgg
    48541 tcagcggagt tttttgcatc aattccacaa tagcctgatt gtatttattc tttatttcaa
    48601 cgagacaatc tttcatttga ttgtttttca atctacttat agcaaaatct agcaattttt
    48661 tatctgctaa atatttttcc gacttaaaaa tcttgcccca aaaattattc tttaatctat
    48721 cataggcatc tatcaacacc tgatcattgc tagccttaat ttcttgatag gtcatgctgc
    48781 tatttcggta acgatttcta gtctctctta ttttactagc cgtttcatta cgcaaagatt
    48841 gggcatgatc tttggaaaat ccccgagtaa attgtgacga atttatcatt agctctggtc
    48901 gctctggcat attttttatt aaccttattt tatagtgttt gttagaaaaa atcagaactc
    48961 attttgccta aaatactgaa tgctggccgc aatgtttcgt gccaatagaa attggcgcgc
    49021 cgcctttaaa attttatcag ttttttgtat ttctcggcag cttcattata gcttactttt
    49081 tccggcattg ttactaatat ttgctgtatt tccgaattgt ttctgctaaa ttgtaataat
    49141 tcttcttgtg tttttagttg tgaaacttga tcaatcaatt catcgctcaa gccgtagtga
    49201 tcttttattt cattttttat ttgttcctga tctcttcctt ggcttatcat catctgttta
    49261 actaatgcac cgaaaaaata cctctccgga ttagatattt gttctgacga atttttagag
    49321 ccataaaatt taatggcatc gataatgcct accggttttt tggaaaattc ctcgatttta
    49381 tctacaacat tcctatgttt ggcaatagta gtttggtcgc cagcttttat tttttctaaa
    49441 tcttcaggcg taatctcaaa aatttgactt accgttttct gatcaaaata tctttctaat
    49501 ttttgtcgcg cttctgtagt ttggtcgcca gcaataaaat tctctaaagc cattctcatg
    49561 tctccggctt cctcgttgta agtcgttggc atcttttctt tgagaacagc tgctatagca
    49621 taggcatatt ctttaccaga acttccaaaa ggtttataaa tatcatgaaa atctttgtat
    49681 ggttcatctc ggtcttcgga ttttaaatcc caaccgcgaa ttctagtttc tggataaact
    49741 gctaaggctt caatttgggg atttggtgta atatatttgc ctttttccga tttatttctt
    49801 ccgacatact gaaaagcgct ctcgtcaatc tcagctggtt ttccccctcc cgcatccatt
    49861 atcttaataa ttgctagcgg atggcgtata tatgttccaa ctctgaacat ttttcctgaa
    49921 ccacctttaa aattttcaat aacacctgtt tttccttcca tttcacgacg aaagtgatca
    49981 cgcgcagtgt tataaatccc aattaggtga ttcattaaaa gaatttgcgc gcgttgccgc
    50041 ctgcttttaa tattcggcaa atttttacag gcctcatcgt ttattctttc ttccgagttc
    50101 caatcatcaa gcagtctttc catcacccga tcacgatcaa tttgttcact ctctaaacta
    50161 atatcaaaat catcccttcg ttcaccagcc aacaactgaa cactttcatt gttaaaagcg
    50221 tcaattatgc ccctgatcca ttgagaatta actttggtat cggcatccag gcttgcaata
    50281 attccatcgg tttttcctgc taatacgaat ctttcacaaa tttcgtccat ggttcgtttg
    50341 cgtggctgca tacacccgat ttttttaaaa tcctcgtcat caatcggttt tatttcatta
    50401 cctccgctcg atttatcgat ataatgaaca actaagccgc tttctttaat tttctgcaat
    50461 ctttctgttt cttcggtcgt taaaaaagct aaatcagatg gctctccttt taaaaatttt
    50521 agaacgctta aggtttgcat gtttaaatgg cgcgtattat ttcctgcctc ttctcgatta
    50581 ttcacattga caataatctc atagtcatcc attgaaacat ccttttgatt cgccaaaaat
    50641 tccaatgagt ttaagatctt gcctccttct tcgtatgccg ggatgcttac tgccactttg
    50701 cattcctgac gcattggctc agcaattctt gcctctgttt taggcaattc tttggcaata
    50761 tattctaaaa tatttttttg tttttctagc tgttctcttg tctcctgact ttttacaatt
    50821 ttctctatag tcatatcgat attgttgtgc attatttgtt tcttactgtc agcgctcaat
    50881 aattgaccca aactattttc tatatctttt atttgccttg tggtctcatt ttctttttca
    50941 tctctgacta aatcaatatt tttatctaat ttaggcagga cactaagttc gtcgtcaatt
    51001 cttgctaata gttcctgcct tcgctctatt gaagattttt cagacacatt aagctgttct
    51061 ggtgtttttc cagcttcaat atttggctgt tctaattttt gatttggcgg gtcgctcata
    51121 ttattgaaca aaatcaccta tatttactgc taatgttttg gcgattctac aaggttttta
    51181 ttgttgaact gataatttca ccggttttaa actttattat ggaattaaaa gaaacatccg
    51241 ctaatcttga gaggcgatct tggggcaagc cctactatta aaatacatcg tttctctaat
    51301 ttttacaaag gaatttactg ttgtgggcgc agagtggacg ggccaggata atttcttttt
    51361 tcttgacagc gtatttcact tgacgaaata cgaaaaattg cgacggcgaa aacgccgagc
    51421 gagactaatt caatatggtg gaccttaagg gatttgaacc cttgacctcc tgcatgcaaa
    51481 gcaggcgctc tagccaactg agctaaaggc ccaacactaa gtattttttt ggtaacggat
    51541 taatggacca agcaaccagc ccataaaatt attaattaac ccgaaagcaa tagaaaagcc
    51601 aataaaaata gcaatcaagc caattaataa ataggccaga cgagttccgc catagatccc
    51661 gagtttttcc tcaaaaaatt ccattcggcc aaaattattt agaaaccatt cacgcttcat
    51721 aatgattagg gcgccaatcg ccacaatcac caatccgcct aaaaaacgca ataaaaacat
    51781 agcttaacgg atataattca aaggattata tctaacttta ccagacaaaa cctcaaagtg
    51841 caaatgcggt cctgtggagc ggccagttga acccatctca ccgataacct gacccttaac
    51901 taccgtctgg ccagcccgaa cataaagcct agataaatga ccatagcgag tttttagttt
    51961 gccatgattc agtaaaatag tattaccata accgccattc cagccgttgg ccgccacttc
    52021 caccttgccg ccatcagcgg cataaatcgg tgtaccgacc ttattgccaa tatcaacacc
    52081 attatgacgc caagaataat attgcgtaat acgatggccc accgttggcc aagtcatgcc
    52141 gccagtccct aagttggcct tgaccgtatt tttgacatta ctgaagacct gctgaacttt
    52201 tggcgttaat ttactgctgg ccacggccac ttgactggcg actgaggctg gtggttgtcc
    52261 accaggtata attaacgact gaccaataat tacccgatcg ccagttaaat tgttatagct
    52321 aataatagcg gcggcttcaa ccttgtactg ttgggcaatc ttagataggc tatcgccggg
    52381 acgaacttta tataagacgc cgcttaccgg caaaataacc aatttatcgc caggtcgcaa
    52441 cagcgcttta gcgtttaaat tattttgcca agcgatggta ttaacagtca cgccaaaggc
    52501 tttagctacc ccactcaaag tgtcgccaat tttaaccgta taataagtaa tagatcgccg
    52561 tccgccagcc gcgccggccg ccggttgatt gctgctcggt aaactagcca aacccaaact
    52621 ataaacccaa cgatcagtgg ccatgccaac taaaccagcc ggaccagggt cgctgattcc
    52681 caaagtagat aatttagccg aaacttccgt agattggtta ggtgctagct ggtagccagc
    52741 ccgatcatca accacaaaat tattcaattg attaaattca ctgcccacca atttggacaa
    52801 ccaagtccct ctgactaaat cttcagcggc tgtcagtcgg ccgctagcgc gccaattgcc
    52861 gaaaacaaca ccaatcgcca ccacagcaac aaccgtcacg gtcaaccaaa gattttgctg
    52921 ataatgatta attaatctcc ttttaattcg cagataacca cgatataacc aaactgctgg
    52981 ctgataaatt attaaccaat taaataattt taataaacga gaaaacagcc aaaaacttat
    53041 ttgcttgact tttaataaaa tattaattat caatataagt cctttgatgg ctaatccggc
    53101 cactgaacgg actggttgtg gtttggcaat atgtttttag tcttaatagc tggcctaact
    53161 ctagcatttt tattccctat cgtcaattat aaccaacaaa tatgggctaa aaataacaat
    53221 tactaattag acttgtcggg tagccgcttt tttgttagac tggttttgtt ttctggtcac
    53281 gtcgccaaga ggctaaggca acggtttgca aaaccgtcat ccgtgggttc gactcccacc
    53341 gtgacctcta ttaactctag ccaactcctg tggataactt gtgaatttta ttagatttta
    53401 gtctatagcc acctagacaa aggctcaaaa ccacgctcaa tctcaatagt ccacaaccta
    53461 gtagacactt gtaattattg atgattacga caatagtcca cacataccta ccctaaacta
    53521 ttgacgactt tttctttttt tgctatactg tggatatcgt tctttctaga cttcttctct
    53581 ggtctctccc aaaaaaaaat aaaaacaaaa aattggaccc agtgtttcta taaaagaaac
    53641 cgcaaggttc cctgagaccg gttaagaata tttgttcttg aaatttttgc aaaaaatttc
    53701 aaggggaagg ccgtcggagc tattttctat tattacttta tgtaaaatga agggctcgtc
    53761 tgtggccttc cttttgttta taaactccag cttgacgatt tgtttttttt tacctacaat
    53821 aatcagcatc aacttgttta ccgaggacag ccctcatagt tcaatggata gaacaaggga
    53881 ctcctaagcc ctagatccgt gttcgactca tggtgagggc gcaggaataa taattaaata
    53941 tttatgggcc attagctcag ttggctagag cgcttccatg gcatggaaga ggtcaagggt
    54001 tcaagtccct tatggtccac
//
LOCUS       DAOPAI010000004        42624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_1036_length_42624_cov_6.657568, whole genome shotgun sequence.
ACCESSION   DAOPAI010000004 DAOPAI010000000
VERSION     DAOPAI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 42624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42624
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1036_length_42624_cov_6.657568"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..640
                     /locus_tag="PKV49_01105"
     CDS             284..640
                     /locus_tag="PKV49_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPL22245.1"
                     /translation="MSNHNQLWGAYGEELSSAYLSRHGYQIIAQNWRCHHHEIDLIAQ
                     NHNTLIMVEVKLRLVNNFAPAANALSWHKIQQLKKAANYALYFYHPKGWRYDFIAIDL
                     NPKNRLARLCHYRQII"
     gene            637..1524
                     /gene="dprA"
                     /locus_tag="PKV49_01110"
     CDS             637..1524
                     /gene="dprA"
                     /locus_tag="PKV49_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPL22246.1"
                     /translation="MNLLTTNQSSIKQVKNNQANFPKLLNNIYHPISQLWYRGNLALA
                     NQNNLLAVVGSRQSSWYGQLIINRLLDHHITSQLVIVSGLAVGIDSLAHQASLNSNNP
                     TIAVLGSGLDPNSIYPPSNWQLAEKIVNSGGLLLSEYPPGQSARNYHFPQRNRIIAGL
                     SAAVLVIEAAAKSGALITAELALQEGRTVLAVPGNINQTLSAGTNKLIQLGAQAILDS
                     TDLAIALDLPQPTSYSKTNLPITACHLALLKLLTKPLSLDELLQITHQSVSQISQQLT
                     DLELGGHIKQQADQTYVAC"
     gene            1534..3594
                     /gene="topA"
                     /locus_tag="PKV49_01115"
     CDS             1534..3594
                     /gene="topA"
                     /locus_tag="PKV49_01115"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPL22247.1"
                     /translation="MRLIIVESPTKAKTISRFLGKTDIVESSFGHVRDLPTKKMGIAI
                     DNNFEPSYEIPTKAKKTVNKLKALAKQADEIVLASDGDREGEAIAWHLQQLLKLPPKD
                     TNRIVFHEITESAIKTALKNPRQLNQNLIDAQQARRVLDRLVGYELSPFLWKKVAKNL
                     SAGRVQSVAVRLIVEREREIENFQQQEYWTINADLQAKDGQFSAQLARWQDKSLDKLT
                     LSNETLANEIKNKLLNAQYTISSITKNRLTKKPAAPFTTSTLQQTANRWFGFSAKQTM
                     VIAQQLYEGINLGPHGQTGLITYMRTDAVNLSNDFIAQSREVLQNNFGPQFLSPSPRK
                     FANKSKGAQEAHEAIRPTDPQRTPESIKSYLEPRQLKLYQLIWQRALASQAAEAQIDH
                     ITVDIAAGEGLFRATGQTIAFPGYLMIYPEQTQEKLLPSLTANEILQLLNLQTEQHFT
                     QPPARYSDAGLVKQLEKHGIGRPSTYAPIIATIEARNYVIRDEHKRLEPTAIAKVVND
                     LLVAHFPAIVDYQFTANIEERLDQIARGHLNWQPVIADFYQPFHQNLEIKNKQLSRQD
                     ILPDQASTEVCDKCGAPMVIKTSRYGPFLACSAFPKCRNIKSMKEKKVSKPLPEKFQH
                     LLTKYADQVCDKCGAPMAVKVGRYGPFLACSAFPKCRNLQKIIVKTAAATTAVKKNKK
                     SKKI"
     gene            complement(3600..3998)
                     /locus_tag="PKV49_01120"
     CDS             complement(3600..3998)
                     /locus_tag="PKV49_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22248.1"
                     /translation="MELFTTSKDLVNLALAIGVVGISAAIVYFFISAAGFIKEMRQTI
                     DEINRQLNNINQITESVKNKITYMFSYWAVLEKLANKAISLVQKNLINKVKDKFNRYT
                     APTSSVENDPVTDSSSDKQPKRRSARRSNK"
     gene            complement(4003..4401)
                     /locus_tag="PKV49_01125"
     CDS             complement(4003..4401)
                     /locus_tag="PKV49_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22249.1"
                     /translation="MFRWFKKIFQSPIVSQDRFPLIVVGKIIEVNQHPQADRLRLAKV
                     DVGREILNIVCGAPNIEPGQLVPVAMVGAQLPGGLTIQPAELRGQMSYGMLCSAKELG
                     LGDDHSGIMLLNILQVRQLGESLDSYLARQ"
     gene            complement(4412..4882)
                     /locus_tag="PKV49_01130"
     CDS             complement(4412..4882)
                     /locus_tag="PKV49_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22250.1"
                     /translation="MKLKYYLVSNLIVAIIAWLLWLFILFRVDPFAVNWFGRSLFYLT
                     LFVAIFSTMSLIGFIGRFSLVHQKLAHQAVVAAFRQAGLLAFGVVVTLWLLSQQLFNW
                     FNLLILVGGLAMLEFFMLSCQPASGRCFVQKPTAFTADPEIISSSFEDKTPSNR"
     gene            5013..6185
                     /locus_tag="PKV49_01135"
     CDS             5013..6185
                     /locus_tag="PKV49_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPL22251.1"
                     /translation="MPDKPSHSFPKGFLWGTATSSHQIEGGTLNDWTIWEPGHTHDGS
                     ISGAACDSYRRFKEDIGLAKKINSNAYRFSIEWSRIEPRQQQFSQQAIQHYQEVVDEC
                     RRQKIEPVVTIYHFTQPIWFAELGGWNNPAAPQIFNNYVLYLAQNLKRVKIWCTINEP
                     GLYVWNAYGQGKWPPQKYGLWSWWTASNNLIKSHRLAYRTLHLMQKNCQVGIANNSQA
                     IEPINNHCLNRFIGRIANYLINDHFFQKTAGCHDYLGINYYFHRQIGLGRRPKNQQWH
                     NDLGWEIYPQGLYQVLMAAKKYNLPIYILENGLADSQDQHRQQFITEHLAMVYRAIHD
                     GCQVKGYFHWSLLDNFEWKWGFKPRFGLYAVDYQSLERRPRPSSRTYAKICKNNGF"
     gene            6189..7136
                     /locus_tag="PKV49_01140"
     CDS             6189..7136
                     /locus_tag="PKV49_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPL22252.1"
                     /translation="MWLLITVLANLFNGAVYLADKYFLSKKVHSSISYTFYVGIWSVG
                     NCFLFLFNRHVPTWPWLLLDLAAGLVFMVALYFWYKALHQGETTKVVPIAGAFIPIFT
                     LLLNYLIGRHNLTGQELGALVILIIGSGLLSARPKDNQWLAWWIRLKRFFQPNRVIFR
                     STRRLIFNSLLASFIFAIYYVFIKFLYDSQGFSNAFIWTRLGSFLGALIIIAWPPSRR
                     LILDKPRRQTTIKSLPLFLLIRIIAFIAFVLSNFAIALADVSLVNSLQGLQYLFLLII
                     VLILNKRYPKLVKQEISRDNLWQKISGIVLINLGLFLLL"
     gene            7133..8155
                     /locus_tag="PKV49_01145"
     CDS             7133..8155
                     /locus_tag="PKV49_01145"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPL22253.1"
                     /translation="MIKKIRRRWRWIIVGLTTLIILGILWTWLTKRDIYQPDQMSYGL
                     TFSPLVLQDMGLDWRAAFIEILDDLAVKRLRLVAYWPMVEPIKNNYDFSDLDWQIEQA
                     EQRNAQIILAVGARLPRWPECHYPQWYNAENEAERQQQLLSYLQTVIERYDHNPAIAA
                     WQIENEPFLIGFGECPKLNVKLLDQEIALARSLTKKPIVITDSGELSIWLPAAKRADI
                     FGTSIYRHVYSKYLRHYVTYPMTPNFFRIKTKLVELFTKTKNLIVIEMQAEPWGRQAY
                     FTISPAERNRTMNMEKLQKMLEFSRQSGFQTIYLWGVEWWYWEKQVNHNSEYWQFIKN
                     LLAQSV"
     gene            8152..8997
                     /locus_tag="PKV49_01150"
     CDS             8152..8997
                     /locus_tag="PKV49_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPL22254.1"
                     /translation="MNKKELLIGLDIGGSKVLGLLYDRQTGKSYAAKRQLINKEKLSD
                     LLNQLNIEINRLSMEAIRLQSKIAGIGIGIPGSINNGKIIRCPNLPILNGQKLGQLLA
                     PAVKINNIVLDNDLNCFLRAYLQKHPALKKQTLVVIALGTGVGGSIAINGQNIIKHLG
                     ISSELGHLIVDQSGHDLEYYYHQLTGHPSGRLFTAAEDGNQTAQRKIDQFANILGLVV
                     ANLNTIFNPNSIILTGGVSRYHKFYLDQVKKIMKNYSFTNNRPVWRIGHDQKAGAIGA
                     SLLIN"
     gene            complement(9023..11926)
                     /locus_tag="PKV49_01155"
     CDS             complement(9023..11926)
                     /locus_tag="PKV49_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPL22255.1"
                     /translation="MPIPKLVVRKPAPAKRFVNQTVNRSALIVRAKKPSRQRSTKLRQ
                     RLDWPKIIKFFGALILIMAIIGGIAFLWFSRGLPDPNKLLDRQVVQSTKIYDRQGETI
                     LYEIHGDQKRTLVNLDQIPDHVKQATISIEDKDFYKHGGFSLWAMFRTVVTNVLLGRH
                     AGGSTLTQQFVKNALLTSEKTYTRKAKELILAYRIEKRFSKDQILQMYLNEIPYGSNA
                     YGVEAASQRYFGKSVNQVNLAEAAILAALPQAPSRYSPYGTGKDMLIARQHYVLDVMV
                     KQGYISEDEAIVAKNFEVQFKPLKENGNMIAPHFVMAIKDQLAEKYGEKMIEQGGLKI
                     YSTLDIEKQKIAEEVLTAQAEKNAKNFNASNAALLSIDAKTGDILAMVGSKDYFNDEI
                     DGQVNLVLRPRQPGSSFKPIVYAAAFMKGYTPRTVLFDVTTNFSLDPDKAYQPSNYNG
                     QEHGPVTMKKALAGSLNIPAVKTLYLAGLDNVLAVAKNLGYTTLTDKSRFGLSLVLGG
                     GEVKLLEHVAAYSAFARDGEMVRPRYILKIEDDRGKVLEETKVEQQKVLEPQIAREIN
                     DILSDNNERAYIFGAKNYLTLNNRSAATKTGTTNDYKDAWTIGYTPSIITGVWVGNND
                     NKSMSRGADGSVVAAPIWNGYMNRVLSGPAENFKKPETETTGKAVLDGVMPTTTAVMI
                     DKVSNLLATSSTPPDMIKEDRYWQGHSILYYINRDDPRGSAPNNAAADPQFELWEAGV
                     KNWATKQKFNMAEPPTEKDNLHTIENLPDLTIISPTAGQRITDNSLPVKLKVTAPRGV
                     KEVHFYINGLLLKTNSSEPFDDKLSLDSLPNGQHKLAVEVCDDIKNCSKHELTFDLNI
                     ANNQTNSQSLSLAWQSPADGLTFRAADFPINLQLRINQAKLAAKIKVYYSIKNGQRQF
                     IDAVLPDSDGLASLNFKQPSEGNGYYSVIAEAELWSGQTKTSPPLGLIIKGLK"
     gene            12084..12476
                     /locus_tag="PKV49_01160"
     CDS             12084..12476
                     /locus_tag="PKV49_01160"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22256.1"
                     /translation="MNQVKKFLFGLLALQCLALSPIAIAPARAQVDLWGGNNIANNAF
                     NSIGFSRKDPREIVANIIKILLGFLGTIAVVLIIYAGFLWMTAAGKPDNTKKAKDIMS
                     AAVTGLVIILISYGITVFVTNALSNAMH"
     gene            12516..12914
                     /locus_tag="PKV49_01165"
     CDS             12516..12914
                     /locus_tag="PKV49_01165"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22257.1"
                     /translation="MKKLFVGLTSLVWAASLLSLVPVASQAANTITSNDLWGHNTVGA
                     YQNEGFSTTDPRQTVARIIKVALGFLGSIAVILIILAGFKWMTAAGNEDKVAESKKLM
                     SAAFVGLVIILTAFALTNFVINSVITSIGH"
     gene            13003..13362
                     /locus_tag="PKV49_01170"
     CDS             13003..13362
                     /locus_tag="PKV49_01170"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22258.1"
                     /translation="MSRQLMIIKRVVYLFGFYFLPIIARADNFGLDRAANSAGLKTEG
                     TLAEKSGQIIGSLLSLVGVIFLILMVYGGITWMTASGNDKSVEKAKNIITYAVIGLII
                     ILSAYTITAFLGGTLAK"
     gene            13364..13771
                     /locus_tag="PKV49_01175"
     CDS             13364..13771
                     /locus_tag="PKV49_01175"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22259.1"
                     /translation="MFWQNKKNLLIIFLIILSAIGLAAKAESNSITANNAFGYFFGSA
                     QKTAEQMGYVANTTDGTSLLLIKVAALIGFALSLVGVIFLILMVYGGITWMTASGNDK
                     SVEKAKGTISRAAIGLIIVILAYAITLFVINRL"
     gene            13774..14082
                     /locus_tag="PKV49_01180"
     CDS             13774..14082
                     /locus_tag="PKV49_01180"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22260.1"
                     /translation="MAANLKDAFSDNQLNQTAVAAGYDPTQNNALTTVGNFITIILGL
                     IGVIFLALIIYGGFLWMTAAGNDKQVDKAKAIISQATVGLIIIILAYAITFFIIKILP
                     "
     gene            14087..14494
                     /locus_tag="PKV49_01185"
     CDS             14087..14494
                     /locus_tag="PKV49_01185"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22261.1"
                     /translation="MSKTNILVIRLVSLIIAVGFIGVPVAKAVPPATLDNLKIVASPS
                     YQNSTNENTLFDNASLIIKTILELLGFIFVVLMIYAGILWMTAGGNEKQVEKAKNIIK
                     QALTGLIIVVLAYAITYFIFTRLPGGGLGTGPA"
     gene            complement(14546..15058)
                     /locus_tag="PKV49_01190"
     CDS             complement(14546..15058)
                     /locus_tag="PKV49_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22262.1"
                     /translation="MRNTIALILLVILLAGCSVNRNTTRAEIRLHASSQQLLAVNASG
                     SMPVNDWLETRSQQSLVETKVNYLTRAQQAATPAESQQWLDLAAQVGGGGSTTLAILV
                     NASDYWVTVLDGPYAGIRLAPGASSRSTARVPVGWLSFVVAWGDNQRASIVRPVSAGQ
                     KAIVIVNKQQ"
     gene            15238..16554
                     /gene="nusA"
                     /locus_tag="PKV49_01195"
     CDS             15238..16554
                     /gene="nusA"
                     /locus_tag="PKV49_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPL22263.1"
                     /translation="MSELMSAIKLICEEKNLSYDAVIETIEAALAAAYRKDYGDKNQN
                     IKVNYNEDLSKIKVFDVKTVVEDMPLPAFSAPLTLKNLQDSTITIDPEAAKEAVNFQT
                     EDQLQAEIRDENGEIIRKFNPKTEIQLCEAQLIKPGIKLGEEIITELPVQTDYGRMAA
                     QTAKQVIIQKLREAERNMIYEDFKDKEHEVISGIIQRREGRNILVDVGKTAGIIPPQH
                     QIAGEAYRPNSRIKVFVEEVRVSNRGPEIVLSRTADEILSKVFYLEIPEISNGLVDLK
                     AVAREAGSRSKVAVIANSDNVDPIGSCVGQRGSRIQTIINEVGGEKIDIIEYSDDAAK
                     FIANSLAPAKIEQVELDHETKQAVITVDPEQLSLAIGRDGQNVRLAARLTGWHLDITT
                     NQDQQLIDDEPEPIIEPEKEVVKEKKVKKTTTKATVKKTTKKTAAK"
     gene            16571..17605
                     /locus_tag="PKV49_01200"
     CDS             16571..17605
                     /locus_tag="PKV49_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22264.1"
                     /translation="MFFFRYGEDDFQIKRAIRQQLADFNQSAVEGAIHLNAAEIDSSV
                     LFNAIGNQSLFQQTKIIILDNPFSSKNAELLDGLCVLLNNHEQKSTAWLLLINETNLK
                     LKYQAGKNRPVIVDIEGRSRPLTKAQEKLFTLLTADTKQQQYYPRLTGLAAENLLETL
                     IKEHGGQMAPSAKKLLLELTNYNFWQISHELNKLINYQASINPKIVIDDDLVRLLVND
                     TSRHLFEFIEAFSRQDLSTSAGLLEEIFANEDDLAISVALLNRQALQFLQIKARLAAG
                     QTTTSLEKSLGLPISVSRKLIQQANLIQLVSLQQLVDNLTKFDWSKKRSRGNLAALFT
                     LLLINHANQK"
     gene            complement(17612..18748)
                     /gene="dnaN"
                     /locus_tag="PKV49_01205"
     CDS             complement(17612..18748)
                     /gene="dnaN"
                     /locus_tag="PKV49_01205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPL22265.1"
                     /translation="MKLTILQKNLHQGLNTVGHIATKNANMPILNNVLISIKDGLINL
                     SATNLEIAITKQIRGKADKDGEITVDAKILSDYIALLPNEKIDISLDKQELKINCQQY
                     NTVIKGQEAQDFPVIPQVDKKIGFNLSVASLKNALSQILFAINPGDNRLELTGVLFDI
                     KNKTMNLVATDSFRLAKTELDFSLNLGQISADGLKVIIPFKTMQEISRIINSTTDELS
                     GDADQLKLFLSDNQAMFVYQGTTIISRLIEGHYPDYEQVMPINFKTEAVIDRGELSRA
                     IKMASIFSTNITNAIDITINSLEQKIIIEAVGGQAGSQQADLPAKIDGQNNNVRLNYR
                     YLLECLNVLSAGQVIIKLIDDVAACQISVPKDDNYQYIIVPLRK"
     gene            complement(18955..20376)
                     /gene="dnaA"
                     /locus_tag="PKV49_01210"
     CDS             complement(18955..20376)
                     /gene="dnaA"
                     /locus_tag="PKV49_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPL22266.1"
                     /translation="MEHPSLEQIWQAILGEAELTISKANFTTWFKNSFISSYENSVLT
                     IGVPNGFTRAWFETKFSQEIVHIFNKITGEKPTDIKYIIKLKPQQTASITNFLTTENQ
                     KSTDSALTTNHQPIKTAPEPKLTANQQTGLVAGLNPRYTFETFVVGKHNELAHAACWA
                     AVRQPGETYNPLFIYGGVGLGKTHLLQAIGHELIKSGKKVLYVTSEKFTNDYVEAVYH
                     GRGNTFKSLYRNVDLLLIDDIQFMAGKDGTQQEFFHTFDQLHRANKQIVITSDRPPRS
                     MKAVEARLITRFEWGMVADISQPDLETRMAILNTKCQQKQFPLDQSVVQYIATNINNN
                     VRELEGALNRIMGFCQFHNCSPDIAVAQQVLKDFFISIQTSNLSTPQKILEAVANFYN
                     TNVTELTGQSRKRELVGPRQIAMYLLRTKINASFPAIGQELGGRDHTTAMYACAKIEA
                     DLKTNNQLKQDLEQIAQAITSTY"
     gene            20574..20711
                     /gene="rpmH"
                     /locus_tag="PKV49_01215"
     CDS             20574..20711
                     /gene="rpmH"
                     /locus_tag="PKV49_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPL22267.1"
                     /translation="MPKRTYQPNNRRAHKKHGFLKRMLTKNGRNVLRRRRNKGRKKLT
                     K"
     gene            20735..21079
                     /gene="rnpA"
                     /locus_tag="PKV49_01220"
     CDS             20735..21079
                     /gene="rnpA"
                     /locus_tag="PKV49_01220"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPL22268.1"
                     /translation="MLKKQFRLTKKKDFDRLFQADSHKYYGNKLAVRLAANGLSYNRF
                     GFIISRHLCRLAVGRNLIRRRLAAVIKQDYSKLQPGYDIVILLLTKQPTIKLSDLQEE
                     LTKAWKRLRIIV"
     gene            21076..21339
                     /gene="yidD"
                     /locus_tag="PKV49_01225"
     CDS             21076..21339
                     /gene="yidD"
                     /locus_tag="PKV49_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPL22269.1"
                     /translation="MMFIRTLDNFLAAVACRVIRFYQKSLSPDHGFLSRRFSSGYCRF
                     RPTCSEYSLAAFKKYGFLKGGLLSIGRILRCHPWSKGGWDPLR"
     gene            21354..22067
                     /locus_tag="PKV49_01230"
     CDS             21354..22067
                     /locus_tag="PKV49_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPL22270.1"
                     /translation="MGHIFTIIFYQPVLNLLVFFYNILPGHDIGLVIVALTVAIKLIL
                     WPLSQRALKSQQVISQLQPKIKEIQNKYKDNKEEQGRAMMALYREAKVNPTSSCLPLL
                     IQLPFLWAVFRVFRDGLSNKSLALVYPFISRPELLQTTSFGIIDLSKPNIFLAILTGL
                     AQYFQAKTATRQTPPAAGGQAEELSSIMNKQMLYFMPVMTVAICWRLPSGLSLYWFLS
                     SILTILQQIYWFKKHKHNN"
     gene            22082..23056
                     /locus_tag="PKV49_01235"
     CDS             22082..23056
                     /locus_tag="PKV49_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22271.1"
                     /translation="MILYRQIIKQAWHNTIKNSWLWVAGVLAALLTNVGQYSNLFSSL
                     DGTNSWLRINQSLVEFWSKATMVVKGAISSPLLLLICLLIVVLVMVVLFLAVNSQIFV
                     VKQVKLGLKSGEQPLPRPKASLWKQLVSNWPIFWPAVGAIVAVKILLLVCLLIISALM
                     AAIYAIHQPIISSFLYVLLSLLALASIWLIAVWARYWILLLLTSKKGNLLLSARQAWE
                     LLKRNILVSLEMSIIIALINLLAYLLWIFVIYLLAIPFVLLTLILIKYLAVSQLLLIT
                     IANILLIASLLILVGFMVVFEVSLWLNFMNNLDNQKIMSKLKRIFKRR"
     gene            23218..25188
                     /gene="infB"
                     /locus_tag="PKV49_01240"
     CDS             23218..25188
                     /gene="infB"
                     /locus_tag="PKV49_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPL22272.1"
                     /translation="MNVTELARHLRVSPQELRDILPRIGFDIGQRAIKIDQRLAQRII
                     KEWPRLVRELKNREERERRQEEAVAAVRAEQRVVKIGSVVTIKDLANSFGLPVNRILA
                     ELMKNGIFASLNEKIDADTAMIIGSELGIEVVVENAADNKKDEVDLLEESRRNQGDQA
                     DLEVRPPVIVVMGHVDHGKTKLLDAIRTTDVVATEAGGITQHIGAYQITRKGKVVTFI
                     DTPGHEAFTAMRKRGARVADLAILVVAADDGVKPQTIEAYRIIEEAKIPFVVAINKID
                     KPEANIEKAKQELANQLNLLAEDWGGKVVICPISAKQRLGIEDLLDTILLLADLEESS
                     RLANPKLEAIGTIIESHVNKSEGPVATVLVQNGTLKIGDTLICNEQYYGRVRALKNYR
                     GQNIKQAGPSTPFKIIGLKATPEIGDVVRVGQADRSNKVKVATKSYNQAITRVHDNGS
                     FGGQTINLIVKSDVFGSGEAIEESLLKLNNEEVAVRIIHRSLGNITEGDITRAEANQA
                     RIIGFSVKASAQVEDLARSKEVVIEQYEIIYDLINKIKKEMQDLMQPEIRRVDLGRLK
                     VLAIFHFEGDGQVVGGKVLDGSIKADCQLEIVRNKEIIGQGKLLKLQSGRQDVSEILV
                     DQEGGLQVSSQTTIEVGDILQAYREDKLFKKM"
     gene            25191..25520
                     /locus_tag="PKV49_01245"
     CDS             25191..25520
                     /locus_tag="PKV49_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HPL22273.1"
                     /translation="MAVDNRRLNDLLRQKIAELVNQLVEFYNGLITVSYVNLSGDGQE
                     AKVGVSVLPIGLYGTALRQLRKNSAEIAKRAARASRLSKIPRIIWLIDDTAERAADLD
                     RVMEQVN"
     gene            25524..26489
                     /locus_tag="PKV49_01250"
     CDS             25524..26489
                     /locus_tag="PKV49_01250"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPL22274.1"
                     /translation="MDQALAKRAWQVLRQAEKWLIITHHNPDGDALASTNIFLEIAAQ
                     LDKPALAYGANQPSDNFGFLSKTSQLTDQLDYKQFKQFSVVIVVDCGSASRTKLADWL
                     KQDLPGQTIIEFDHHEPQDNYADISFKESQAAATVEVIHDFLTAIRWPINTEIARSIL
                     TGLVTDTGNFLYSNTTEKTTKLAAKMMLLGARLPKIINQTLASKNLAVMRLWGRALSR
                     LKINPDYLLAVVIIQQKDLLEFGLTSDDLSGLIGLINSLQGVTGNLLLVETDDGQLKG
                     SLRTNQINVDMSFLASLLGGGGHQKAAGFTIPGKIIGDNDQLVIA"
     gene            26549..26782
                     /locus_tag="PKV49_01255"
     CDS             26549..26782
                     /locus_tag="PKV49_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22275.1"
                     /translation="MEKIFKTICGGLNKIIVSLTIVGVLLLVLSFLTVWTDFMAKLVL
                     GMVILLLAYALLSTAYRLWKFSQDLNNFWKIKK"
     gene            26786..27289
                     /locus_tag="PKV49_01260"
     CDS             26786..27289
                     /locus_tag="PKV49_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22276.1"
                     /translation="MAKKKINKSVWFWLIIVLGVAVLAVISWRIIPRRQPINMDELGW
                     DNLYHYRNQELNFSLDLPEQFIYYQTERTNSADYREIRFYVPTSDPDYLYEALPSYAK
                     PIIVRVYDEQAWNALSDNEESKSDFQKITIKNGQVYLIRFWDRQPKDWQDKWSDEMKA
                     AISQSLK"
     gene            27381..30062
                     /gene="polA"
                     /locus_tag="PKV49_01265"
     CDS             27381..30062
                     /gene="polA"
                     /locus_tag="PKV49_01265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPL22277.1"
                     /translation="MKATKEKLVVVDGNAVIYRSFYAVQSGLSYNGQATNAVYGFFSV
                     LFKVVSELQPQYLLVALDRPEPTFRHQLSVDYKAQRIKQPDELYSQIPIIKQLLKTMA
                     VPVLELAGYEADDIIGTVVNRVPPSVQCYIVTGDKDSFQLVNEQVSVYWLKQGLQKTV
                     ILNQEGVKQVLGLEPRQVVDYKALRGDPSDNIPGVPGIGDKTALDLLKSFDTLDKLQE
                     VLAVDLVDERIKPRWRQLIKDNQASLDLSQQLATIDCNVPLDFVWPVARFGDFDIITV
                     RQAFLDWQLRSLIPRLEKLADRSIKSVTQPPAVARLPIIAVKNINESARLAAEYTEKT
                     INLLPLWDGERLTKLILGSSDQLLEFTVAGPAQQELFQSSADEPVDIIKPWQPIFENK
                     QVKKIIFQSKEVSKFLFKSAIKLAGLAGDPLLANYLLNPGARQASLVDIGQQVGFNLE
                     PAGDALTMTDNYYQNCGQLTAIHQKLAEQLKANSLLDIYQRIELPLTKVLAVMELNGI
                     KIDQARLISLAKEWQAKLAKLTQEIYRQAGQEFNINSPKQLQVILFDHLKLSSKGLAK
                     TKSGPSTDAANLQKLKQQHPIINLLIEYRELSKLLNTYALALPKLINHNDGRLHANFQ
                     QAITATGRLSASEPNLQNIPTKTEIGKKLRQAFIADPGCQLISFDYSQIELRIMASLA
                     NEPSMIADFAAGRDIHLATAAKINDIRLDQVSDQQRRAAKAVNFGLLYGQGPHGLAEA
                     TGLSYSAAKDFIDQYFVIYPRIAEYMNKAIDQARHWGFAATVWGRRRYLPDLDSPNQA
                     IRRAAERMAINLPIQGTAADIMKAAMIAVDDWLSKNFNQRQARLILQIHDEILIEAAS
                     EEVDKIMTAVPKLMTDVIDLTVSLVVSAKTGFNWAEL"
     gene            30067..31392
                     /gene="murD"
                     /locus_tag="PKV49_01270"
     CDS             30067..31392
                     /gene="murD"
                     /locus_tag="PKV49_01270"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPL22278.1"
                     /translation="MINWQDYSAKRIAVLGLGSENEALILWLAEQNVAIDITIYDRRP
                     LEAIADKKEKIMRAVGPLTVSWRLGEQSYDSLEDYDELWRSPGWPIFCPQVQGALQNG
                     RRLNSPMNLFFETCPRQRMIGITGSKGKGTTSSLIANILEAAGQPVLLGGNIGVPPFS
                     FINRINDQSWVVLELSSFQLEDLQYSPHIAVITNIYEEHLAPADPLNPNYHKTPEDYW
                     LAKQQIFRQQKAGDWLVVNYKLENKLDNNLINSQVKFFSELNWPSHLVGQHNQENIAA
                     AQAVIEIIGLSEKISRQAVANFKGLEHRLEFVATKDGVSYYDDSFATNPIATTIAIKA
                     FNRPVILLAGGADKGNNFRPLAQTIVEHSVKTVILFDGTASPRIKSDLLATGYPEEMI
                     LSAGSMDQAMQLAKQKAVDGDVVLLSTACASFGIFNNYKERGNLFKQAV"
     gene            complement(31376..33199)
                     /gene="ftsH"
                     /locus_tag="PKV49_01275"
     CDS             complement(31376..33199)
                     /gene="ftsH"
                     /locus_tag="PKV49_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPL22279.1"
                     /translation="MKNLLKNFFLVLIVFLSISYLFSLYNSRSAKIEKVGIESLVQEI
                     NNDQITAITIDGDTLNINLKDNRRQIAVKEPGESLSQLLRNFEVAPEKMAAISIKVKN
                     NTTSNLLITTILPLALPLILIIGLFYFVMRSAQGANNRAISFGQTTAQEIDRRKNKIT
                     FKDVAGVKEAKEELQEVVQFLRQPKKFTDLGAKIPKGVLLLGSPGTGKTLLARAVAGE
                     ANVPFFSLSGSEFVEMFVGVGASRVRDLFKKAKKSAPCIVFIDEIDAVGRRRGAGLGG
                     SHDEREQTLNQILVEMDGFEVGTNVIVMAATNRPDVLDPALLRPGRFDRQVILDEPDL
                     KDREAILKIHADNKPLTKNADLHLVAQRTPGFSGADLANVLNEAAILAATKNKKVIDN
                     EELLMSIEKVLLGPERKSRILSDKEKLITAYHEAGHALVAHYLPHTDQVHKISIIARG
                     QAGGYTLKLPDRDKHLQSKQEFLEEIAVLLGGYLTEQLVYGEVTTGATSDLRRATNLA
                     RKLVTDFGMSDKLGPRTFGQKEEMIFLGREIREQRDYSEKIAEEIDQEINNFIQTGRN
                     LAEHFIQKHRTELDKISQELVVKETIEKEAFEKLVGPKPLA"
     gene            complement(33300..34571)
                     /locus_tag="PKV49_01280"
     CDS             complement(33300..34571)
                     /locus_tag="PKV49_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPL22280.1"
                     /translation="MNQTNDKSEFAKLLANDQLVIPQVGDTITGKIISASKSEVKLDI
                     NGVMTGIIRGRELYFDASDYAKLPAGEEIEATVVDTDNENGELELSFRHAGEQKAWVN
                     LQESYSNKTNLKVRVLDANKGGLIISLGQITGFLPVSQLAPENYPRVSGGDKTKILEK
                     LKTLVGSELEVRVMTLDEEEKKLIVSEKDAWQDKQKDIISRYQVGSVVEGAITAITGF
                     GLFINFDNNLEGLIHISELAWQRIDDINELYKIGDTIKAEIISIDGSKIFLSAKKLIA
                     DPWKNVAKKYAVGQKVTGLVLKVNPFGLFVELDNDIHGLAHISQLSLNPGQKITDAYT
                     PNTQAEFTIISLEPDSHRLGLANVVKKNKADQDAKESIESTPKETKKTTKKTTKAATE
                     SQPKTEADIKPKKTPAKKTTKKADTKETPAE"
     gene            34842..35459
                     /locus_tag="PKV49_01285"
     CDS             34842..35459
                     /locus_tag="PKV49_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22281.1"
                     /translation="MDYSRSKIRLFNKKQTRQISSLLVAVVAFELLFFPWPTFAANQD
                     EQSSSTAETLSFNLDANNIIPRDLTNDRWSLRLPPEEVLVEDVKKVEGQFSGRLTKTR
                     QVVLTAYNSDKAQCDDSPCITASGFNVCQHGIEDTVAANFLPLGAKIKIPALFGDRVF
                     VVRDRMAKRYQNRVDIWMKEYSAAIKFGIKTATIEILPAELAINK"
     gene            complement(35521..35826)
                     /gene="rpsT"
                     /locus_tag="PKV49_01290"
     CDS             complement(35521..35826)
                     /gene="rpsT"
                     /locus_tag="PKV49_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPL22282.1"
                     /translation="MPNLKSAKKELRKSKSRTAHNEQVRQEIKKMVKGSRRAIDAKTA
                     EAVAMVKQTIKTLDKAVKKGVIKKNTASRKKSRLQKRLNVGQPIAAAKPKAKSKTKK"
     gene            complement(35877..36680)
                     /gene="lgt"
                     /locus_tag="PKV49_01295"
     CDS             complement(35877..36680)
                     /gene="lgt"
                     /locus_tag="PKV49_01295"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPL22283.1"
                     /translation="MYWLTTTPSPIIFTLGPINFYWYGLTMVIAMLAAGFLTWTLAKK
                     HRWPLNNLLDLGFNIIIGGLIGARLYHILIHWSDYAGNYLDILKVWQGGLAIHGGLIG
                     GLITLWLTSRRRQWPFWQLAGWLMPGVALGQAIGRWGNFFNQELFGQPSTMPWAIPID
                     IWHRPLNSITASHYQPLFLYESIACLLLAILLYQLNRRQVAANKIVGVYLLGYGAIRL
                     IMEFFRQDPTWVLWGWRWPQWLSLLLIILGSLLLTGWPKLIRLAKKPSS"
     gene            complement(36835..37209)
                     /locus_tag="PKV49_01300"
     CDS             complement(36835..37209)
                     /locus_tag="PKV49_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22284.1"
                     /translation="MGIQKTKVNSGEEKMPNLKIALLTLLALAIFAGIMLATSPKQTI
                     IINGIDNKVYTGIINDLYNSPNPDSLAQSSPDRFRWAMDYLEEFDNINLTGKPSGETS
                     VFFYRLSKDMGKNFLINGKPVN"
     gene            37539..38471
                     /locus_tag="PKV49_01305"
     CDS             37539..38471
                     /locus_tag="PKV49_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HPL22285.1"
                     /translation="MPQSNQEEKMRWIKPLLLKELTIKQMAQVSPFSERTLKYWLARF
                     REAGEAGLENKSRRPKSQPNELPIRIKEKIIELRHETKLCAQKLNYKLIKEGIAVSDR
                     VIGKIIKTEGLVRKYRVRKLKYKYIKVPLAIGELVEIDIKFVPNKIRGRRYYQFTAID
                     CASRWRYLKIYEDCGNGSAIAFVKRLIKIAPFRVRAIKTDNGSCFTNYYTGYKKSSDP
                     FNPRLHPLDIFLTSRNIIHYLIDPGKPAQNGKVERSHRTDQEMFYNRHRFKTAVELKQ
                     EIKKWNISYNNLEHIALGGLTPNEALARVQNVCA"
     gene            complement(38527..40902)
                     /gene="ppsA"
                     /locus_tag="PKV49_01310"
     CDS             complement(38527..40902)
                     /gene="ppsA"
                     /locus_tag="PKV49_01310"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HPL22286.1"
                     /translation="MTKYVKFFNQLGIKDVPVVGGKNASLGEMYQKLTKSGIRVPNGF
                     ATTAEAYRHFLKDTGTQQKLRKILDGLNTHDVVDLAKRGQAARQAILKAELPKDLQKE
                     IIIAYRKLSGFYKTKNLSVAVRSSATAEDLPDASFAGQQETYLNVSGEKDILIAVKKC
                     LASLFTNRAISYRVDKKFDHFSIALSVGIQKMVRSDLASSGVMFTIDTETGFTNAVVI
                     NSIYGLGENIVQGKVSPDEFYVFKPTMAIISKSLGEKKIKMIYAAKNASNPVKDVAVN
                     IKDQQRYSITDQQVKSLAKWGLIIEKHYKRPMDIEWALDGHDKRLYIVQARPETVNSQ
                     RNYNILEEYIIKKRGTVVVEGRSVGNRVGAGIVNRIMSVKDISKFKPGQVLVTEMTDP
                     DWEPIMKIASAIVTDKGGRTCHAAIVSRELGIPCVVGTSTGSQKLANGQTVTVSCAEG
                     DTGFVYEGKLPFSIKKTDIQKLPRPACKIMLNIGSPDIAFASSFLPNDGVGLAREEFI
                     VNNFIRIHPLALINYSKIKNAKLKKQIDELTVGYTDKKQFFIDKLAEGVGTIAAAFYP
                     KDVIVRLSDFKTNEYANLIGGQDYEPIESNPMIGWRGASRYYDPKFEPAFALECLALK
                     KVREDFGLKNLKVMVPFCRTIEEGKLVIKTMAKYGLKQGRGGLEVYVMAEIPTNILLA
                     SDFAKVFDGFSIGSNDLTQLTLGIDRDAGNLDIAGISNEKNSSVKSLIQFLIEVGQKT
                     HTKVGICGQAPSDFPDFAQFLLACGIDSISLNPDTVIKTTLSLGKFKPRKK"
     gene            41094..41175
                     /locus_tag="PKV49_01315"
     tRNA            41094..41175
                     /locus_tag="PKV49_01315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41128..41130,aa:Leu,seq:tag)
     gene            41187..42386
                     /locus_tag="PKV49_01320"
     CDS             41187..42386
                     /locus_tag="PKV49_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22287.1"
                     /translation="MLNFDPTILQTIQQSGRVLVAADIQSSPSNGAAAVALVSGLNQA
                     GIQADFVANGQLDDHWSVLGSFNQPNQSLASEDFIISLDLSRTKVGQIKYKIEGERLN
                     FLIKPSNGQFKEEDVELLVGRLPYDLVITVGVADPEVLGDIYNNNSALFYQTPVINID
                     CQTKNENFGQINLVDLVASSAAEVVYDLWCALQWPLTPDQATCLLAALIYDTKNFTTA
                     KASPQVLLAASNLIREGARREVIVNQWYSAVALPTLRLWGKVLSGLEQADNGLVWSKL
                     DVGDEMVSEIAIGQAVERLLTGLQDAKVVAIFYNLNQSPEPTVSLSLLKNQASLKEIN
                     QSLSQTGSQTGVVIYANGAMNLREWLQEFSPISHGQMISFKSNLPTSSTMELILPLLS
                     RRLSEIN"
     gene            42432..42504
                     /locus_tag="PKV49_01325"
     tRNA            42432..42504
                     /locus_tag="PKV49_01325"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42465..42467,aa:Ile,seq:tat)
     gene            42550..42623
                     /locus_tag="PKV49_01330"
     tRNA            42550..42623
                     /locus_tag="PKV49_01330"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42584..42586,aa:Val,seq:gac)
BASE COUNT        12654 a         8258 c         8912 g        12800 t
ORIGIN      
        1 tagagcgctt ccatggcatg gaagaggtca agggttcaag tcccttatgg tccaccaccc
       61 ccaccatagt tccatgtgga actatggtga tttattttat taacagttga cggtttgtcc
      121 aagaatatat agacctaata accagtggaa aagtccaaaa atatatagac aaatcataaa
      181 ttttgtacta atattagaga agttatttct agtggataag ttgtgaatta ttttaaataa
      241 agtccagaat aacctaccat ttatttatct ccccagtcaa tttatgtcca atcataatca
      301 gctctgggga gcttatggcg aggaattatc tagcgcctat ctatcgcgcc acggttatca
      361 aataatcgct caaaattggc gttgccacca tcacgaaatt gatctaatcg ctcaaaacca
      421 caacacatta attatggttg aggttaaatt acgcttagtt aataattttg ctccagcggc
      481 caatgcttta agctggcaca aaatccaaca gttaaaaaaa gcggcgaatt acgctttgta
      541 tttttatcat cctaaaggct ggcgctatga ttttattgct attgacctta atcccaagaa
      601 tcgcttggct cgactctgtc attatcgtca aattatatga accttttaac caccaaccag
      661 tcatcaatca agcaagttaa aaataatcaa gcaaatttcc ccaaattact aaataatatt
      721 taccatccga tatcacagct gtggtatcgc ggtaatctcg ccttagccaa tcaaaacaat
      781 cttttagctg ttgttggtag ccgccaatcc agctggtatg gtcaattaat tattaaccgc
      841 ctgcttgatc accatattac tagccaactg gtaattgtta gcggattggc tgtgggaatt
      901 gatagtctag cccatcaagc cagtctaaat tctaataatc caactattgc tgttctcggt
      961 agcggtcttg atccaaacag tatttacccg cccagcaatt ggcaattggc tgaaaaaatc
     1021 gtcaacagcg gtggtctctt gttatcagag tatccaccgg gtcaatccgc gcgtaattac
     1081 cattttcccc aaagaaatcg gattatcgcc ggattatcgg cagccgttct ggtaattgaa
     1141 gcagccgcca agtccggtgc tttgattacg gccgagctag ccttacaaga aggacgaaca
     1201 gttttagcag ttccaggcaa tattaatcaa accctctcgg ctggtactaa taaactaatc
     1261 caattaggcg ctcaagccat tctggattca accgacctgg cgattgcgct tgacttgcct
     1321 cagccaactt cttatagtaa aactaactta ccaattactg cttgccactt ggctttacta
     1381 aaattattaa ccaagccgct cagtttagac gaattactgc aaattactca tcaatcagtt
     1441 agtcaaatta gtcaacaact taccgacctt gaattaggcg gacacatcaa acaacaggcc
     1501 gaccaaactt atgtcgcctg ctagctaaaa aatatgcgtt taattattgt tgaatcgcca
     1561 accaaggcta aaacaatcag tcgtttctta gggaaaaccg acatcgttga gtcgtcgttc
     1621 ggccatgttc gcgacctgcc aactaaaaaa atggggattg ctattgataa caactttgaa
     1681 cctagttatg agatcccgac taaagccaaa aaaactgtta ataagctcaa agcgctagcc
     1741 aaacaggccg acgaaattgt cctggcctcc gatggagatc gcgaaggaga agcaattgct
     1801 tggcatttgc aacaattatt aaaattacca ccaaaagata ccaatcggat tgtttttcac
     1861 gaaatcaccg aatcggctat caagacggca cttaaaaatc cccgccaatt aaatcaaaat
     1921 ttaattgatg cgcaacaggc gaggcgagta ctagatcgct tagtcggtta cgaactatcg
     1981 ccttttttgt ggaaaaaagt cgctaaaaat ttatcagctg gccgtgttca gtcggtcgca
     2041 gtccgcttaa ttgtagaaag ggaaagagaa atagaaaact ttcaacagca agaatattgg
     2101 actataaatg ccgatttaca ggctaaggat ggccaattta gcgctcaact ggctcgttgg
     2161 caagacaaaa gtctggacaa attaactctc agcaatgaaa cgctggctaa cgaaattaaa
     2221 aacaaactac tcaacgccca atatactatc agctcgataa ccaaaaatcg tctaactaaa
     2281 aagccagccg ccccatttac cacctctact ttgcaacaaa ccgccaaccg ctggtttgga
     2341 ttttcagcta aacaaaccat ggttattgct cagcaacttt atgaaggtat taacctcgga
     2401 ccccatggac aaactggctt gattacctat atgcgcactg atgcggttaa tttatctaat
     2461 gactttattg ctcaatctcg cgaagttctg caaaacaatt tcggccccca atttttatcg
     2521 ccaagcccaa gaaaatttgc caacaaaagc aagggggcgc aagaggctca tgaagctatt
     2581 cgaccgactg atccgcaacg aacaccagaa tcaataaagt catatttgga accgcgacaa
     2641 ttaaaacttt accaattaat ttggcaaaga gccttagcga gccaagcagc tgaagcacaa
     2701 attgatcata ttactgttga tattgcggct ggtgaaggat tattccgggc tactggccaa
     2761 accattgctt tccccggcta cttaatgatc taccctgaac aaactcaaga aaaactattg
     2821 ccatcattaa cggctaatga aattttacaa cttttaaact tgcagaccga gcaacatttt
     2881 acccaaccgc cagcccgtta tagcgatgcc ggacttgtta aacagctaga aaaacacggc
     2941 attggccgac catcaactta tgcccccatt attgcaacaa ttgaagctcg caactatgtt
     3001 atacgcgatg aacataaaag gctagaacca accgccattg ccaaagtggt taacgatctg
     3061 ctggtggctc atttcccggc aatcgttgac taccaattta ccgccaatat tgaagagcgc
     3121 ttagaccaaa tcgctcgtgg ccatttaaat tggcagccgg tgattgccga tttttatcaa
     3181 cccttccatc aaaacctaga aataaaaaat aaacaattgt cccgtcaaga catcctgcca
     3241 gatcaagcca gcactgaggt ttgcgataaa tgcggcgcac caatggttat taaaactagc
     3301 cgctatggtc cattcttggc ttgcagcgct tttcccaaat gccggaatat taagtctatg
     3361 aaagaaaaaa aggttagcaa accgctgccc gaaaaatttc agcacctatt aacaaaatat
     3421 gccgaccagg tttgcgataa atgcggcgcg ccaatggcag ttaaagtcgg ccgctatggt
     3481 ccattcttgg cttgcagcgc ttttcccaaa tgccggaatc tacaaaaaat tatcgtcaaa
     3541 acagcagccg cgacgactgc tgttaagaaa aataaaaaaa gtaaaaagat ttaacttatc
     3601 tacttattgc ttcggcgcgc tgaccggcgc ttcggctgct tgtcggacga gctatcggtc
     3661 acaggatcat tctccacaga actggttggt gcagtatagc gattaaattt atccttaacc
     3721 ttattgatca agtttttttg cactaagcta atggccttat tagccagttt ttctaacacc
     3781 gcccaataag aaaacatata agttatctta tttttaactg attcggtaat ctgattaata
     3841 ttgtttagct gtcgattaat ttcatcaatc gtctggcgca tctctttaat aaagccggcc
     3901 gcgctaatga aaaaataaac tatcgccgcg ctaatgccaa caacgccgat ggctaaggct
     3961 aggttaacta agtctttaga ggtagtaaat aattccataa aattattggc gcgccagata
     4021 gctgtctaaa ctttcaccta actggcgaac ttgtaaaata tttaataaca taatgccgga
     4081 atgatcatcg cccaagccca gttctttggc cgaacacaac atgccatagg acatttggcc
     4141 gcgcaattcg gccggttgaa tagtcaaacc gcccggcaat tgagcgccaa ccatggcaac
     4201 aggaaccagt tggccaggct caatattagg cgccccgcaa acaatattta aaatttcacg
     4261 accaacatca accttggcta aacgcaaccg atcggcttgc ggatgttgat tgacctcaat
     4321 aattttacca acaacaatta gaggaaatct gtcttgagaa actattggag attgaaaaat
     4381 ttttttaaac caccgaaaca taacttaata tctagcggtt agaaggcgtt ttatcttcaa
     4441 aactagagct gattatttcc ggatcggcag taaacgctgt cggcttttgg acaaaacacc
     4501 ggccactggc cggttgacaa ctcaacataa aaaattccaa catcgccagc ccaccaacta
     4561 aaattaaaag attaaaccaa ttgaatagct gctgcgataa taaccataga gtaacaacca
     4621 ccccaaaagc caaaagaccg gcttgtctaa aggcggccac cactgcctga tgcgctaatt
     4681 tctgatgaac taaactaaac cgaccgatga agccaattag agacattgtg ctgaaaatag
     4741 caacgaataa agttaaataa aataggctcc ggccgaacca attgacggca aaagggtcaa
     4801 ctcggaataa aataaataac cacaatagcc aagctataat agcaacaatt aagttactga
     4861 ctaaataata ttttaatttc atgttttcaa atactgacca ataaatatat aatactataa
     4921 atactcaaaa accgcaaaat attgccatta aattgtccga ccataaaaaa aatgatatac
     4981 tgctgatatc aatcaataaa atcagtaatt acatgccaga taaaccaagt cactcttttc
     5041 ctaagggatt tctttgggga acagccacat cgtcccacca gattgagggt ggcactctta
     5101 acgattggac catttgggaa ccgggccata ctcatgacgg atcaatttcg ggagcagctt
     5161 gtgattctta tcgtcgattc aaagaagata tcggtctggc caaaaagata aatagtaatg
     5221 cttaccgttt ttcaattgag tggagtcgca ttgaaccacg acaacaacaa ttttcccaac
     5281 aagccatcca acattatcaa gaagtggttg acgagtgtcg acgacaaaaa attgaaccag
     5341 tggtcaccat ttatcatttt actcaaccaa tttggttcgc tgaattaggc ggctggaata
     5401 atccagcagc gccacaaatt tttaataatt acgttctcta tttagctcaa aacctaaaac
     5461 gggtgaaaat ctggtgcaca attaacgagc ctggcctgta tgtctggaat gcctacggac
     5521 aaggaaaatg gccgccacaa aaatatggcc tatggtcttg gtggacagcc tctaataatt
     5581 taattaagag tcaccgcctg gcttatcgaa cactacatct gatgcaaaaa aattgccaag
     5641 tcggtattgc taataatagt caagctattg aaccgattaa taatcactgc ctgaatcgtt
     5701 ttatcggacg aattgctaac tatttaatta acgaccattt cttccaaaaa actgccggtt
     5761 gccacgatta cttaggtatt aactattatt ttcatcgtca aatcggttta ggccggcggc
     5821 caaaaaatca acaatggcac aatgatttag gctgggaaat ttacccgcaa ggcctttatc
     5881 aagtgctaat ggccgctaaa aaatataacc tgcctattta tatcttggaa aatggtttag
     5941 ctgattcgca agatcaacat cgccaacaat ttattactga acatttagca atggtttacc
     6001 gggctattca cgatggttgt caggtcaaag gttattttca ttggtcatta ttggataatt
     6061 ttgaatggaa gtggggattt aaaccacgct ttggtcttta cgcggttgat taccaatcac
     6121 tggaacgccg accgcggcca agtagtcgga cttatgccaa aatctgtaaa aataacggct
     6181 tttagactat gtggctactt atcactgttt tggctaattt gtttaacggg gctgtctatc
     6241 tagctgataa atacttttta tctaaaaaag ttcactcttc catctcctat actttttatg
     6301 tcggcatttg gagtgtcggt aattgctttc tattcctctt caaccgccat gtcccaacct
     6361 ggccatggct attgctagac ttagccgccg gtttggtatt tatggtcgct ttatatttct
     6421 ggtataaagc tctccaccag ggagaaacca ccaaagttgt cccgattgcc ggcgctttta
     6481 ttccaatttt tactttactg ttaaattatt taatcggccg ccataatttg actggccaag
     6541 agctgggcgc tctagttatt ttgattattg gtagtggatt attgtcggct agaccaaaag
     6601 ataatcagtg gttagcttgg tggatcaggc taaaaagatt ctttcaacca aatagggtta
     6661 tttttcgcag cacaagacga ttaattttta attctttatt ggcctcattt atttttgcta
     6721 tttactatgt attcattaaa tttttatatg actcccaagg attttccaat gcttttatct
     6781 ggactcggct aggtagtttt ctcggcgctc tgataataat agcttggcca cccagccgac
     6841 gcttaatttt agataaaccg cgccgacaaa caacaattaa aagcttgccg ttatttttac
     6901 taattagaat tattgccttt atcgcttttg ttctcagtaa ttttgctata gctctagccg
     6961 atgtttcctt ggtcaacagc ctacagggat tgcagtattt atttttactg ataattgttt
     7021 tgattttaaa taagcgctat cctaaactag ttaaacaaga aattagtcgc gataatctgt
     7081 ggcaaaaaat tagcggtatt gtcctaatta atttaggctt attcttattg ctatgataaa
     7141 aaagatacgt cgccggtggc gctggataat tgttgggcta accactttaa ttattttagg
     7201 aatattgtgg acttggctca ccaaacgaga catctatcag ccagaccaga tgagttatgg
     7261 cttaactttt tcaccattag ttttgcaaga catgggatta gattggcgcg ccgcctttat
     7321 cgaaatattg gacgacttgg ctgttaaacg actacggctg gtggcctatt ggccaatggt
     7381 tgaaccgatc aaaaataatt atgattttag tgatttagat tggcaaatag aacaagcgga
     7441 acaaagaaac gcccaaatta tattagcagt tggcgcgcgc ttgccccgct ggcctgaatg
     7501 tcattatccc caatggtata atgccgaaaa cgaagctgaa agacagcaac agttattgtc
     7561 ttatttacag actgttatag agcgctacga ccacaaccca gccatcgccg cctggcaaat
     7621 tgaaaatgag ccatttttaa tcggctttgg cgaatgtcct aaattaaatg ttaaattatt
     7681 ggatcaagaa atagctttgg cgcgcagttt aactaagaaa ccaattgtta ttactgatag
     7741 cggcgaacta agcatttggt tgccggctgc caaacgagct gatatttttg gcacttcaat
     7801 ctatcgccac gtttacagca aatatttgcg tcattatgtc acctatccaa tgactccaaa
     7861 ttttttcagg attaaaacta agcttgttga gctttttact aagactaaaa atttaattgt
     7921 tattgaaatg caggctgagc cctggggacg ccaagcttat tttaccattt cgccagctga
     7981 aagaaatcgt acgatgaaca tggaaaaatt acaaaaaatg ctggaattca gtcgccagag
     8041 cggttttcaa acaatttatt tatggggggt agagtggtgg tattgggaaa aacaagttaa
     8101 tcacaactca gaatactggc aatttataaa aaatctgtta gctcaatcgg tatgaataaa
     8161 aaagaactac taatcggcct ggatattggc ggttctaaag ttttgggcct actttatgac
     8221 cgacaaaccg gcaaatctta tgcggctaag cgccaactaa ttaacaaaga aaaattatcc
     8281 gatctattaa accagcttaa tattgaaatt aatcggcttt ccatggaagc catccggctg
     8341 caatcgaaaa tagctggtat tggtattggt attcccggat caattaataa tggtaaaatt
     8401 attcgctgcc caaacttacc aatacttaac ggccaaaaat tgggccagct tctagctcca
     8461 gccgttaaaa taaataatat cgttctagac aatgatttaa actgtttctt gcgagcttat
     8521 ttacaaaaac accccgcctt aaaaaaacaa accttggtgg taattgctct gggcaccggt
     8581 gttggcggat caattgctat taacggccag aatattatta aacatttagg aattagcagc
     8641 gagctaggtc accttattgt tgatcaatca ggccatgatt tggaatatta ttatcatcag
     8701 ctaactggcc atccatccgg tcgattattt accgccgctg aagatggcaa tcagacagct
     8761 caaagaaaaa tcgatcaatt cgccaatatc cttggtttag tggtggctaa tcttaatact
     8821 atctttaacc cgaactctat tattttaacc ggcggagttt cccgctatca taagttttat
     8881 ctagatcagg ttaaaaaaat aatgaaaaac tatagcttca ccaacaaccg tcctgtctgg
     8941 cgaattggcc atgatcaaaa agctggcgct atcggcgcca gcctcttgat taactaaaaa
     9001 ccacttaatc tttttaagct aattatttta agcccttaat aattaatccc agcggggggc
     9061 tggtttttgt ttggcctgac cataattcgg cttcagcaat gacagaataa taaccattac
     9121 cttccgatgg ctgtttaaaa ttaagactgg ctaggccatc gctatcaggt aaaacagcat
     9181 caatgaactg ccgctgacca ttcttaatac tatagtagac cttaatttta gcggctaatt
     9241 tagcttggtt gattcttaac tgtaaattga ttggaaaatc agccgctcga aaagttaggc
     9301 catcagctgg agattgccaa gccaatgata atgactggct attagtctgg ttgttggcga
     9361 tatttaaatc aaaagttagc tcatgcttag aacaattctt aatgtcgtca caaacctcaa
     9421 cagctaattt gtgctggcca ttgggcaggg aatcaagact tagtttatcg tcaaaaggtt
     9481 cgctggaatt agtctttaat aatagaccgt taatataaaa atgaacttct ttaacgccgc
     9541 gcggagcggt aacttttaac ttaaccggca aactattgtc agttatccgc tgtccggccg
     9601 ttggactaat aatagttaaa tcgggcaagt tttcaatggt gtgcaaatta tctttttcag
     9661 ttggcggctc ggccatatta aatttctgct tagtagccca atttttaact ccggcctccc
     9721 acagctcaaa ttgtggatca gcggcagcgt tatttggcgc cgatcctcgc gggtcatcac
     9781 gattaatata atacaaaata gaatggcctt gccagtagcg atcctcttta atcatatctg
     9841 gcggtgtaga actggtggcc aacaaattag aaactttatc aatcataacg gcggtagtgg
     9901 ttggcataac cccatctaga acagccttgc cagtggtttc tgtttccggt ttcttaaaat
     9961 tctcggccgg accggataaa acacgattca tgtagccatt ccaaataggt gcggccacca
    10021 cactgccgtc ggcgccacga ctcattgatt tattgtcatt attaccaacc caaacaccgg
    10081 taataattga cggcgtatag ccaatggtcc aagcatcctt atagtcattg gtagtgccag
    10141 ttttggtggc agcgctacga ttattaagtg ttaaataatt cttagcacca aaaatatacg
    10201 cccgctcatt gttatcactt aaaatatcat taatttccct agcaatttgc ggttctaaaa
    10261 ctttctgttg ttcaacttta gtttcctcca aaaccttgcc gcgatcatct tcaattttta
    10321 aaatatagcg aggacgaacc atttcaccat cacgagcaaa agcgctatag gcggccacat
    10381 gttctaataa tttaacttcg ccgccgccca acactaaaga caggccaaaa cgactcttat
    10441 cggttagcgt ggtgtagcca agatttttag ccacagctaa aacattgtct agcccggcca
    10501 gatacagagt tttgacggcc ggaatattca atgaaccagc cagggctttt ttcatggtga
    10561 ccggaccgtg ttcttgccca ttataattac ttggttgata ggccttgtcc ggatcaagag
    10621 aaaaattagt agtcacatca aacaaaaccg ttcgcggcgt atagcccttc ataaaagcgg
    10681 cggcatacac aattggctta aatgatgacc ctggttgccg cggtcttaag accaaattaa
    10741 cctggccgtc aatttcatca ttaaaataat ctttagagcc aaccatagcc aagatatcgc
    10801 cagtcttagc atcaatagac aataaggcgg cattgctggc attaaaattt ttagcattct
    10861 tttcagcctg agccgttaaa acctcttcag caattttttg tttttctata tctaaagtgg
    10921 aataaatctt taatccgccc tgttcaatca tcttttcacc atatttctcc gctaactgat
    10981 ccttgatggc catgacaaaa tggggagcaa tcatgttgcc attttctttc aacggtttaa
    11041 attggacctc aaaattcttg gcaacaatcg cctcgtcttc actgatatat ccctgtttaa
    11101 ccattacatc taaaacatag tgctggcgag caatcaacat gtctttgcca gtaccatagg
    11161 gcgaatagcg gctaggcgct tgcggtaagg ccgctaaaat agccgcttcg gccaaattaa
    11221 cctgattgac gcttttacca aaataacgct gactggctgc ctcaacacca taagcattgg
    11281 aaccataggg aatttcattg agatacatct gtaaaatctg atccttggaa aaacgttttt
    11341 caatacggta ggctaaaatt aattccttag ccttgcgggt atatgttttt tccgaggtta
    11401 ataaagcatt cttgacaaat tgctgagtta aggtcgagcc gccggcatgc cgacccaata
    11461 aaacgttggt taccactgtt ctaaacatcg cccacaagct aaagccgccg tgtttataaa
    11521 aatccttatc ttcaatagaa atagttgctt gtttaacatg gtcgggaatt tgatctaaat
    11581 tgactagagt tctcttttgg tcaccatgaa tctcatagag aatagtctcg ccctggcgat
    11641 cataaatttt agtgctttga acaacctggc gatctaataa tttattagga tcgggcaagc
    11701 cgcgagagaa ccacaaaaaa gcaatgccac cgataatcgc cattattaaa attaaagcgc
    11761 cgaaaaactt aataattttc ggccaatcta gtcgctgtct taatttcgtc gaccgctgtc
    11821 gactaggttt tttagctcta acaattaaag cggagcgatt aacggtttga ttaacaaaac
    11881 gcttggctgg cgccggtttg cgaactacaa gtttaggaat aggcatatta aatatgttat
    11941 tatgataatt atagcaaact gacccgatag attcaaaaac ggctagccga tagttagttc
    12001 ttgttgtctt tttttggtta tgttataatg gcggcagaat ataaatttta atttttagcc
    12061 ctttattggt taaaacaaat tttatgaatc aagttaaaaa gtttttgttt ggattgctgg
    12121 cactgcagtg ccttgcttta agtccaatcg ccattgctcc agctagagcg caggttgatt
    12181 tatggggcgg taacaatatc gccaacaatg ctttcaacag cattggcttc agcagaaaag
    12241 acccacgaga aattgttgcc aacattatta aaattttgct tggctttctg gggactatcg
    12301 ctgttgtctt aattatctat gccggcttct tatggatgac cgcggctggc aagccggaca
    12361 acactaaaaa agccaaagat ataatgtccg cggcagtaac cggcttggtg attattttaa
    12421 tatcttacgg tattacagtg tttgttacca atgccctaag taatgctatg cattaaaatt
    12481 aattaactat taataataaa taaagttaaa aacttatgaa aaaacttttt gtcggcttaa
    12541 catccttggt ctgggccgcc agcttgctgt ctttggtccc agttgccagc caagctgcca
    12601 acactattac tagcaatgat ttatggggcc ataatacggt tggggcttat cagaatgagg
    12661 gcttctcaac cactgatcct cgccaaactg ttgccagaat tattaaggtg gccttgggtt
    12721 tcttgggatc gattgccgtt attttaatca ttttggccgg cttcaagtgg atgaccgcgg
    12781 ctggcaatga agacaaagtt gctgaatcta aaaaattaat gtccgctgcc tttgttggct
    12841 tagttattat tttaaccgct tttgccttga ctaattttgt tatcaactca gttatcacat
    12901 ctatcggtca ttaactgtgt cttaattcgc caaaccagtt tggcgaattt tgtctcaatc
    12961 taaaataatt tggattgaga caaaatttct taaacaaaat ccatgtctag acagttaatg
    13021 ataattaaac gagtagtcta tctatttggt ttttattttt taccaataat tgctcgggcc
    13081 gataattttg gattggaccg ggctgcaaat agcgccggtt taaaaaccga gggcaccctg
    13141 gcagaaaaat caggccaaat cattggttct ctattaagtt tagtcggcgt catcttctta
    13201 atcttaatgg tttatggtgg aattacttgg atgactgcca gcggtaatga taaaagtgtt
    13261 gaaaaagcta agaatatcat aacttatgct gtgatcggct tgataataat cttatcagct
    13321 tatacaatca ccgcctttct gggcggaacg ctggccaaat aatatgtttt ggcagaacaa
    13381 aaaaaactta ttgattattt ttttaattat actttcggct ataggcctgg cggctaaagc
    13441 agagtctaat agcatcactg ctaataatgc ttttggttat ttttttggta gcgcccaaaa
    13501 aacagccgaa cagatgggtt atgtggctaa tactactgat ggcaccagcc tactgcttat
    13561 caaggttgca gcgctgattg gttttgcttt aagtttagtc ggcgtcatct tcttaatctt
    13621 aatggtttat ggtggaatta cttggatgac tgccagcggt aatgataaaa gtgttgaaaa
    13681 agctaaagga actatttctc gggcggctat tggcttaatt attgttatct tggcgtatgc
    13741 aattacttta tttgttatta atcgattata attatggccg ctaatcttaa agatgccttt
    13801 tcggacaacc aattaaatca gactgccgta gcggccggct atgacccgac ccaaaataat
    13861 gctttaacaa ctgttggcaa ttttataact attatccttg gcttaattgg tgttattttt
    13921 ttagctttaa taatttatgg cggttttttg tggatgaccg cggctggcaa tgataaacaa
    13981 gtagacaagg ccaaggctat tattagtcaa gcaactgtcg gccttataat tataatcttg
    14041 gcctatgcaa taaccttttt tattattaaa attcttccat aactttatgt caaaaacaaa
    14101 tattttagtg ataagactgg tttctttaat aatagctgtc ggctttatag gtgttcctgt
    14161 agccaaagct gtgccgccag caacactgga caatcttaaa atcgtagcat cgccgagtta
    14221 ccaaaactca accaatgaaa atactttgtt tgacaatgcc tcattgatta taaagactat
    14281 tttagaatta ttgggcttta tctttgttgt cttaatgatt tacgctggca ttctttggat
    14341 gactgccggt ggcaatgaaa aacaagtaga aaaagccaaa aatataatta aacaagcgct
    14401 tactggtttg attattgtgg tgttggccta tgcgataact tattttatct ttactaggtt
    14461 gccgggcggt ggacttggaa ctggtccagc ctaagaaaaa acccgctcca aagaacgggt
    14521 ttcgtgtcaa aaaaattttt aatcgttatt gttgtttatt cacaataacg attgcttttt
    14581 gtccggccga cactggccgg acaatgcttg ccctttggtt gtcgccccag gcgacaacaa
    14641 aggacaacca gcccacggga actctagccg ttgatcggct agaagcgccg ggggctaatc
    14701 tgatgccagc gtagggcccg tccaatacgg ttacccaata gtcgctggca ttaaccagga
    14761 ttgccagcgt ggtgctacct ccaccgccca cctgggcggc tagatctagc cactgctggc
    14821 tctcagccgg cgtggccgcc tgctgagccc tggttaaata gttgaccttg gtctcaacca
    14881 gactttgctg agaccgggtc tctagccagt cgttgactgg catggagccg ctggcgttaa
    14941 ccgccagcaa ctgctggctg ctggcatgga gccgaatctc ggctctagta gtattcctgt
    15001 tgacactaca accggctaat aatatgacca gcagaataag ggcgatagtg tttctcatga
    15061 aacacctcct ttctttcttt atttatttat tttaataggt acaatattgt actataccat
    15121 aaaataacaa aacggtcaat agttattgac aaatttgctc aacctggtat aattaattta
    15181 ttgctagttt atgattaaac tggctttttt attagccaat ttatttttat caagaatatg
    15241 tcagaactaa tgagcgctat taagctgatt tgcgaagaaa aaaaccttag ctatgatgcg
    15301 gtaattgaaa ccattgaagc cgccttggcc gccgcttatc gcaaggatta tggcgacaag
    15361 aaccaaaata ttaaagttaa ttacaatgaa gatttgtcta aaatcaaagt ctttgatgta
    15421 aaaaccgttg ttgaggatat gccacttccg gcttttagcg ccccattaac tctcaaaaat
    15481 ttacaagatt ccactattac tattgatccg gaggcggcca aggaagcagt taattttcaa
    15541 accgaagatc aattgcaagc cgaaatccgc gacgagaatg gcgaaattat ccgcaaattt
    15601 aatcccaaaa ccgaaattca gctctgtgaa gctcaattaa ttaaaccggg cattaaactt
    15661 ggtgaagaaa ttattactga attgccggtg caaacggatt acggccggat ggctgctcaa
    15721 actgccaaac aggttattat tcaaaaatta cgcgaagctg aacgtaatat gatttatgaa
    15781 gactttaaag ataaggaaca cgaagtcatt agcggtatta ttcagcgccg agaaggacgc
    15841 aatattttgg ttgatgtcgg taaaaccgct ggcattatcc cgccacaaca tcaaattgct
    15901 ggcgaggcgt accggccgaa cagccgtatt aaagtttttg tggaagaagt tcgtgtcagc
    15961 aatcgcggtc cagaaattgt gctatctaga accgctgatg aaattctaag taaagttttt
    16021 tatttagaaa taccagaaat ctccaatgga ttggtggatc ttaaagcggt cgcgcgagaa
    16081 gcgggcagtc gttccaaagt agcggttatc gctaatagtg ataatgttga cccgattggc
    16141 tcttgtgtcg gtcagcgcgg atctagaatc caaaccatta ttaatgaggt tggtggagaa
    16201 aagattgata ttattgaata cagtgatgat gctgctaagt ttatcgccaa ctcgttggcg
    16261 ccagccaaaa ttgaacaggt ggaactagac catgaaacca aacaagctgt tattacggtt
    16321 gatccagaac aattgtcttt agctatcggc cgtgatggtc aaaatgtccg cttggctgct
    16381 cgcctaactg gttggcatct ggatattacc actaatcaag accaacaatt aattgacgac
    16441 gaaccagaac caataattga accggaaaaa gaggtagtta aagaaaagaa agttaaaaaa
    16501 acgactacga aggcaacggt taaaaagaca actaaaaaga cagctgctaa ataattaatt
    16561 agttcaccga atgtttttct ttcgttacgg tgaagatgat tttcaaataa aacgcgctat
    16621 tcgccaacag ctggctgatt ttaatcaatc agctgtcgaa ggagcaatcc atctaaatgc
    16681 tgctgaaata gacagttcgg ttttgtttaa cgctatcggc aatcaatctc ttttccaaca
    16741 aactaaaatt attattttag ataatccttt tagcagtaag aatgccgagc tgttagatgg
    16801 tttatgtgtt ttattaaata atcacgagca aaaatcaacg gcatggttgt tgttgattaa
    16861 tgagaccaat cttaagctta aatatcaagc cggtaaaaat cgaccggtta ttgttgatat
    16921 tgagggcaga tctaggccat taaccaaggc acaagaaaaa ctatttacat tattgacagc
    16981 cgataccaaa caacaacaat attatccgcg attaaccgga ttggcggcgg aaaacttgct
    17041 agaaacctta attaaagaac acggcggaca gatggcgccg tcagctaaaa aacttttatt
    17101 agagcttacc aattataatt tctggcaaat tagccatgaa ttaaataagc ttataaacta
    17161 tcaggcttct attaacccca aaatagtcat agacgacgac ttggtccgtc ttttggttaa
    17221 tgacacctct cgtcacttat ttgagtttat agaagcgttt agccgccaag atttatcaac
    17281 ttccgccggc ctattggaag aaatttttgc caacgaagat gatttagcta ttagtgttgc
    17341 tctccttaat cgtcaagcat tacaattctt acagataaaa gctcggctag cggccggtca
    17401 aacaaccact agtttggaaa aatctttagg cctaccaatt agcgttagcc gcaaactaat
    17461 ccaacaagcc aatcttattc aattggttag tttacaacaa ttagtagata acttaactaa
    17521 atttgattgg tcaaaaaaac gcagccgggg aaatttggca gcgcttttta ctttgttatt
    17581 aattaatcat gctaatcaaa aatagacctg attacttacg aagcggcaca ataatatatt
    17641 gataattatc gtctttgggg acgctaattt gacaggcggc gacatcgtca atcagtttga
    17701 ttattacttg cccagctgat aaaacattta aacactccaa aagataacga taatttagac
    17761 gaacattatt attttgtcca tcaattttgg ccggcaaatc agcctgctgg ctgccggctt
    17821 gtccccccac tgcctcaata ataatttttt gctcaagact attaatagtt atatcaatag
    17881 cattggttat attagtgcta aaaatagagg ccattttaat ggcgcgcgat aattcaccgc
    17941 gatcaataac ggcttcagtt ttaaaattaa ttggcatgac ttgttcataa tctggataat
    18001 gtccttcaat taaccgggag ataattgttg ttccctgata aacaaacatg gcctgattat
    18061 ctgataaaaa taattttaat tgatcggcgt caccggacaa ttcatcagtt gttgaattaa
    18121 taattcgcga gatttcctgc attgttttaa aaggaataat aacctttaac ccgtcagcgc
    18181 taatttgacc aagatttaaa gaaaaatcta attcagtttt agctaaacga aaactatcag
    18241 tggccactaa gttcattgtt ttatttttaa tgtcaaataa aacaccggtt agctccagac
    18301 gattgtcacc ggggttgatg gcaaacaata tttgactaag cgcattcttt aaactggcaa
    18361 ccgataaatt gaagccaatt tttttatcaa cttgtggaat aactggaaaa tcttgtgctt
    18421 cctgtccttt aattactgta ttatattgct ggcaattaat ttttaattct tgtttatcta
    18481 aagaaatatc tattttttcg ttgggcaaca aggcgatata gtcgcttaat attttcgcgt
    18541 caacggttat ttcgccatcc ttgtcagcct tgccacgaat ttgtttagta atagcaatct
    18601 ctaaattggt ggctgataaa ttaattaatc catctttgat actaattaaa acattattca
    18661 gtattggcat attggcattt ttagtagcaa tatgaccaac tgtgtttaat ccttgatgga
    18721 gatttttttg tagaatggtt aatttcatat taatttataa tatataaata aagagataat
    18781 aataaagcct gggtaaaagt tgataactaa tttttaactg ctaacttggc taaaaaataa
    18841 ttattaaagt ggggataaaa aattaataac cagtgggtaa ttagtggaca aaaaagataa
    18901 ttattagtta gctgtggatt attattagtt ttccccaaaa ttataacaac aatgttaata
    18961 agttgatgtg atcgcttgcg cgatttgctc caagtcttgt tttaattgat tattagtttt
    19021 taaatcagct tcaatcttgg cacaagcata catggcggtg gtatgatcac gtccgcctaa
    19081 ttcttgaccg atagccggaa atgaggcatt aattttggtg cgtaataaat acatggcaat
    19141 ttgtctcgga ccaactaatt ctcgtttgcg gctttgaccg gttaattcgg tgacattagt
    19201 attataaaaa ttagccacag cttctaatat tttttggggc gtggataaat tactggtttg
    19261 aatagagata aaaaaatctt ttaatacttg ctgggccact gctatgtctg gtgaacaatt
    19321 atgaaattgg caaaagccca taattcggtt aagagcgccc tctaattccc gaacattgtt
    19381 attaatatta gtcgcaatat attggacaac agattgatct aaaggaaatt gtttttgttg
    19441 acatttggta tttaaaatag ccattctggt ttctaggtcc ggctgactga tatcggccac
    19501 cataccccat tcaaaacgag taattaatcg agcctccact gctttcatac tgcgcggcgg
    19561 tcggtcggag gtaatcacta tttgtttgtt ggcccgatgc agctgatcaa aagtatgaaa
    19621 aaattcttgc tgcgtgccgt ctttcccggc cataaattga atgtcatcaa ttaataacaa
    19681 gtcaacattg cgatataagc ttttaaaagt attgcctcgg ccatgataga cagcctcaac
    19741 ataatcatta gtaaattttt ctgaagttac ataaagaact tttttaccag acttaattag
    19801 ttcatggccg atcgcctgca gtaaatgggt tttacctaaa ccaactccgc cgtaaataaa
    19861 aaggggatta taggtttcac ctggctgacg cacggcggcc caacaggcag cgtgggctag
    19921 ctcattatgt ttaccaacaa caaaagtttc aaaagtataa cgcgggttta gtccggcgac
    19981 caagccggtt tgttgattgg ctgttagttt gggctcaggt gcagttttta ttggctggtg
    20041 attggtggtt aatgctgaat cagttgattt ttggttttcg gtagttaaaa aattagtaat
    20101 tgacgcggtt tgttgaggtt taagtttgat aatatatttt atgtccgttg gtttttcccc
    20161 agtgatttta ttaaaaatat gaacgatttc ttggctaaac ttggtttcaa accaggcccg
    20221 agtaaaacca ttaggaaccc cgatagttaa aacactattc tcatagctag agataaacga
    20281 gtttttaaac caagtagtaa agttggcttt agagatggtt aattcggctt cgcctaaaat
    20341 agcttgccaa atttgttcta aagaggggtg ttccataata tttagattag cagattattt
    20401 ttattataac aggcttggtt ttttcggcaa cccaaaaata agttgataag tttgttaata
    20461 acctgtggac aagtttaggt tcagattaag tattgaccaa agctaaatta gctgttatgc
    20521 tagcagctag actgaaccag tttgaagtta agtcttttta aattcctgac aatatgccaa
    20581 aacgcaccta tcagccaaat aatcggcgcg ctcataagaa gcatggtttt ttaaagagaa
    20641 tgctgacaaa aaatggccga aatgttttaa gacgccgtcg caataagggg cggaaaaaat
    20701 taaccaaata aaactttggt taattttttt atatatgtta aagaaacaat ttcgtttaac
    20761 caaaaagaaa gactttgatc gtctttttca ggcggacagc cataaatatt atggcaataa
    20821 attagctgtt cggctggcgg ccaacgggtt gagttataat cgatttggtt ttatcattag
    20881 tcgccatttg tgccggctgg ctgttggccg caatttaatt cgtcgccgac tggctgctgt
    20941 gattaaacaa gattatagta aacttcagcc tggttatgac attgttattt tattattaac
    21001 caagcaaccg accattaaat tatctgactt acaagaagaa ttaaccaaag cttggaaaag
    21061 attaagaata atagtatgat gtttattaga acattagaca attttttagc cgctgttgct
    21121 tgtcgggtga ttagatttta tcaaaaatca ctatcaccgg atcatggatt tttaagtcgt
    21181 cgtttttcta gtggttattg ccgctttcgt ccaacctgct cagaatattc gctagctgcc
    21241 tttaaaaaat acgggttttt aaagggcggt ttattgtcta ttggacggat tttgcgttgc
    21301 catccatggt ctaaaggcgg ttgggatccg cttcgttaaa ttttaataaa tatatgggac
    21361 atatttttac aattattttt tatcaaccgg tacttaattt attggttttt ttctataata
    21421 ttttgccggg tcatgatatc ggtttagtta ttgtggcctt gacggtggct attaaattaa
    21481 ttttatggcc attatcacaa agagctttaa agtcgcagca ggtaattagt cagttgcagc
    21541 caaaaatcaa agaaatacaa aataagtata aagataataa agaggagcaa ggtcgagcga
    21601 tgatggcgct ttatcgtgaa gccaaggtta atccgacatc gtcctgcttg ccccttttaa
    21661 ttcaattacc ctttttgtgg gcggtttttc gggtgtttcg ggacggatta tccaataaat
    21721 cattggcatt ggtttatcca tttattagcc gtccagaatt gctgcaaacc acatcttttg
    21781 gtataataga tttgtccaag ccgaatattt ttttggcaat tttaactgga ttggctcaat
    21841 attttcaagc caaaacagct acccgacaaa caccgccagc ggctggcggc caagctgaag
    21901 agttatcgtc aataatgaat aagcagatgt tgtattttat gccagtgatg actgtcgcta
    21961 tttgttggag attgccgagc ggtttgtcgt tatattggtt tttatctagt attttaacta
    22021 ttttgcaaca aatttattgg tttaaaaaac ataagcataa taattaaatc aaacaagcta
    22081 tatgattctt tatcgccaaa ttattaaaca ggcctggcat aacaccatca agaatagttg
    22141 gttgtgggtt gccggtgttc tggctgcctt attgactaat gttggccagt atagcaatct
    22201 attcagttcg ttggacggca ctaatagctg gttaagaatc aaccagagtt tagttgaatt
    22261 ttggtctaag gcaaccatgg ttgttaaagg tgcgattagt agcccgctgc tattattgat
    22321 ttgcttgctt attgttgtct tggttatggt ggtgttgttt ttggcggtta atagtcagat
    22381 ttttgtagtt aaacaggtta aattaggatt aaaatctggc gaacagccac ttcctcgacc
    22441 aaaggcgagt ctctggaaac aattagttag caattggcca atattttggc cagcggtcgg
    22501 ggcaatagtt gcggttaaaa ttttattatt agtatgtttg ttaattatta gcgcgttaat
    22561 ggcagctatt tatgctattc atcagccgat tatcagttct ttcttatatg tgcttttatc
    22621 cttgttggcg ctggcttcaa tttggttgat agcagtttgg gctcgctatt ggatattgtt
    22681 gttattgacc agtaaaaaag gcaatttatt gctgtcggct cgacaggcct gggaactact
    22741 caagcgaaac attttggtta gtctagaaat gtcaataatt attgcattaa taaatttatt
    22801 ggcttattta ttgtggatat ttgttatata tttattagct attccttttg ttttactaac
    22861 gctaatacta atcaaatatt tggcggttag ccagttgtta ttaattacca ttgctaatat
    22921 tctcttaatt gccagtttat tgattttggt tggttttatg gtggtatttg aggttagttt
    22981 atggcttaac tttatgaata atctagataa tcaaaagatt atgagtaagt taaagcggat
    23041 ttttaaacgg cgttaaattg aacaaaagat attcaataag caaaaagccc gctaaaagcg
    23101 ggcttttatt atctttttgg gttgactgga ctgaaaaaat attgtaaggt aagagcagaa
    23161 aaatgtttag tttattctgg ccgataatca ctggctagat tttgattatc tttaattatg
    23221 aacgttactg agctagctcg tcatctaaga gtttcaccgc aagagttacg cgatattttg
    23281 ccgcgcatcg gttttgatat tggtcaacgg gcaattaaga ttgatcaacg attggcccag
    23341 cgaattatca aagagtggcc gcgattggtt cgcgaactta agaatcgcga agaacgagag
    23401 cgtcgtcaag aggaagcagt ggccgctgtt cgcgccgagc aacgagtggt caaaattggc
    23461 tcagtggtta ctattaaaga tttagccaat agttttggtt tgccggttaa tcgtattttg
    23521 gcggaattaa tgaaaaatgg cattttcgcc tctttaaacg agaagattga cgccgatact
    23581 gctatgatta ttggtagcga gttaggaatt gaggtggtgg tagaaaatgc cgctgacaat
    23641 aaaaaagatg aggttgacct actagaagaa agtcgccgta atcaaggtga tcaagctgat
    23701 ttagaggtgc gtccgccggt tattgtggta atggggcacg ttgatcatgg taaaactaag
    23761 ctgctggacg ctattcggac gaccgatgtt gttgccactg aagcaggcgg cattactcaa
    23821 catattggtg cctaccagat tactcgtaaa ggtaaggttg tgacttttat tgatactcct
    23881 ggccatgagg cttttaccgc tatgcgtaag cgcggtgcca gagtggctga cttagctatc
    23941 ttggtcgtgg cggctgacga tggtgttaag ccgcaaacga ttgaagctta tagaataatt
    24001 gaagaagcca agattccttt tgtagtggcg atcaataaaa ttgataagcc agaggctaat
    24061 attgaaaaag ccaagcaaga attggccaat caattaaact tattagctga agactggggc
    24121 ggtaaagtgg tgatttgtcc aatatcggcc aagcagaggc taggtattga ggatttatta
    24181 gatactattc ttttattagc tgatttagag gaaagtagtc ggctggctaa tccaaaacta
    24241 gaagcgattg gaacgattat tgaatcacat gttaataaaa gcgaaggacc agtagcgaca
    24301 gttttagtgc agaatggaac actaaaaatt ggtgacacgc tgatttgtaa tgaacagtat
    24361 tatggtcgtg ttcgggctct aaagaattat cgcggtcaaa atattaaaca agccggtcca
    24421 tcaactccgt ttaaaattat tgggttgaag gcgactccag agattggtga tgttgtgcgc
    24481 gtcggtcagg cggatcgcag caacaaggtt aaagtggcaa ccaagagcta taatcaggcc
    24541 attactcggg ttcatgataa tggatcattt ggcgggcaga caattaacct aattgtaaag
    24601 tccgatgtgt ttggtagcgg tgaagctatt gaagaatcgt tattaaaatt aaacaacgaa
    24661 gaggtagctg tgcgaattat ccaccgcagc ttaggcaata ttaccgaggg tgatattacg
    24721 cgagctgaag ctaatcaggc tagaattatt ggttttagtg tgaaagcgag cgctcaagtt
    24781 gaagatttag ctcgcagcaa agaagtggtt attgaacaat atgagattat ttatgatttg
    24841 attaataaga ttaaaaaaga gatgcaggac ttgatgcagc cggagattag gcgagttgat
    24901 ttgggtcgtt tgaaagtatt ggcgattttt cattttgaag gcgacggtca ggtagtcggc
    24961 ggcaaggttt tagatggcag cattaaagcc gattgccagt tagaaatcgt gcgcaataaa
    25021 gaaattatcg ggcagggtaa gttattaaaa ctacaatcag gccgacaaga cgttagcgaa
    25081 attttagttg atcaggaggg tggattacag gtcagcagcc agacaactat tgaggttggc
    25141 gatattcttc aggcttatcg cgaagacaaa ttgtttaaaa aaatgtaatt atggcggttg
    25201 ataataggcg gttgaacgat ttattgcgac aaaaaatagc cgaattagtt aatcagttag
    25261 ttgaatttta taatggctta atcaccgtct cttatgttaa tttaagcggt gacggtcagg
    25321 aggccaaggt cggcgtttcg gttttaccaa ttggtttata tggcaccgcc ttgcggcaat
    25381 tgcgcaaaaa ttcagctgaa atagctaagc gggcggctcg agcgtctcgt cttagtaaaa
    25441 taccgcggat tatctggtta atcgatgata ccgctgaaag agctgctgat ttagataggg
    25501 ttatggagca agttaattag cttatggatc aggcgttagc taagagagct tggcaagtgt
    25561 tgcgtcaagc tgaaaaatgg ttaattatta ctcaccacaa ccctgacggc gatgctttgg
    25621 catcgaccaa tattttttta gagatagccg ctcaattaga taagccagcg ttggcttacg
    25681 gtgctaatca accaagtgat aattttggtt ttttgtctaa gactagccag ctcaccgatc
    25741 agcttgatta taagcaattt aaacagtttt cggtggtaat tgtggttgat tgtggcagcg
    25801 ctagccggac caaattagct gattggttaa aacaagattt gcccggacaa acaatcattg
    25861 aatttgatca tcatgagccg caagataatt atgctgatat tagctttaaa gaaagtcagg
    25921 cggcagctac agttgaggtg attcatgatt ttttaaccgc tattcgttgg ccaattaaca
    25981 ctgaaattgc tcgatcaatt ttaactggcc tggttactga tactggtaat tttttatatt
    26041 ccaacactac tgaaaaaacc actaaattgg cggccaagat gatgctcttg ggcgcgcgtt
    26101 tgccaaaaat tatcaatcaa acgctggcga gtaagaattt ggcggtaatg cgattatggg
    26161 gacgagcgtt aagtcgtctg aaaattaatc ctgattattt gttggcggta gtgattattc
    26221 aacagaaaga tttacttgag ttcgggctga caagcgatga tttatcgggc ttgattggat
    26281 tgattaatag tcttcaaggg gtgacgggta atttgcttct agttgaaacc gacgacggtc
    26341 agctgaaggg cagtttaaga actaatcaga ttaatgttga tatgtctttt ttggccagtc
    26401 ttcttggcgg tggcggccat caaaaagcgg ctggctttac tattcccggt aagattattg
    26461 gcgacaacga ccaacttgtt atcgcttagc gaatattctt aaaataagcc atttgtgcta
    26521 taatcaagtt attaatttta ataactatat ggaaaaaatt tttaaaacta tttgtggtgg
    26581 gctcaacaaa ataattgtta gtctaaccat tgttggcgtt ttacttttag tcctaagctt
    26641 cttaacggtt tggactgatt ttatggccaa attggtgttg ggcatggtta ttttattgct
    26701 ggcttatgcg cttttatcta ccgcttatcg tttatggaag tttagtcaag atttaaataa
    26761 tttttggaaa ataaaaaaat aagctatggc aaaaaagaaa ataaacaaat cagtttggtt
    26821 ttggttaatt atagttttag gggtggcagt cttggcagtc ataagctggc gaattattcc
    26881 tcgccgccaa ccaattaata tggatgaact tggctgggat aatttgtatc attatcgtaa
    26941 tcaagagctt aattttagtt tagatttacc agaacagttc atttattatc aaaccgaaag
    27001 aaccaactcc gctgattacc gggaaattcg gttttatgtg ccgactagcg atcccgatta
    27061 tctttatgag gccttgccga gctatgccaa accaatcatt gtcagagttt atgatgaaca
    27121 agcctggaat gctttaagtg acaatgagga aagcaagagt gattttcaaa agattactat
    27181 taaaaacggt caagtttatt taattcggtt ctgggatcgt cagcctaagg attggcaaga
    27241 taagtggagc gatgaaatga aggcagctat cagccagtca ttaaaataac caatcatcaa
    27301 tcataaaaca tcccgacttc aggggtgttt tattatattt taagctgttc aatgcgctgt
    27361 ttttcccgta taatataaat atgaaagcca ctaaggaaaa actggtagtt gtcgatggta
    27421 acgccgttat ttatcggtca ttttatgcag ttcaatcagg tttgtcatat aatggtcaag
    27481 ccactaatgc tgtttatggg ttttttagtg ttttgtttaa agttgtcagc gaattacaac
    27541 cgcaatattt attagttgcc ctagatcgtc ccgagccaac ctttcgtcac caattatccg
    27601 ttgattataa ggcgcagcgg attaaacagc ctgatgaact atatagccag attccaatca
    27661 ttaagcagtt gttaaaaact atggcagtgc cagtcttgga gttggctggc tatgaggccg
    27721 acgatattat tggcactgtt gttaatagag tgccgccgag tgtgcaatgt tatattgtta
    27781 ccggggataa agatagtttt cagctggtta atgagcaggt tagtgtttat tggctaaagc
    27841 aaggtttaca aaagacggtt atcttgaacc aggagggagt caaacaggtg ttaggattag
    27901 agccgcggca ggtggttgac tataaagccc tgcggggtga cccgagcgac aacattcccg
    27961 gcgtccctgg tattggtgat aaaacagcgc ttgatctatt aaaatctttt gatactcttg
    28021 ataaattgca agaagttttg gcggttgatt tggtagatga acgaattaag ccgcgctggc
    28081 ggcaattaat caaggacaat caagccagcc tagatttaag tcagcaactg gcaacaattg
    28141 actgcaatgt gccattggat tttgtttggc cagtagctag gtttggcgac tttgacataa
    28201 ttactgtgcg ccaagctttt ttggattggc aattacgtag cttaattccg cgtctggaaa
    28261 aactggccga tcgatcaatt aaaagcgtta ctcaaccacc ggcggtggct aggttgccga
    28321 ttattgccgt taaaaatatt aatgaatccg ctagattggc ggctgaatat actgaaaaaa
    28381 cgattaattt actaccgtta tgggatggcg aacgattaac taaattgatt ttaggcagct
    28441 cagatcaatt attggagttt actgtcgccg gcccagctca acaggaatta tttcaaagca
    28501 gcgctgatga gccagtggat attataaagc catggcaacc gatttttgag aacaagcagg
    28561 tcaaaaaaat aatttttcaa tctaaagagg ttagtaaatt tttattcaaa tcggccatta
    28621 agcttgccgg tttggccggc gatccgttat tggccaatta tttacttaat cctggagcca
    28681 gacaagctag tttagttgat atcggacagc aggttggttt taatttggaa ccggcaggcg
    28741 atgcattaac aatgacggat aattattatc aaaattgtgg ccaattgacg gctattcacc
    28801 aaaaattggc ggagcagtta aaagccaaca gcttattaga tatttatcaa agaattgaac
    28861 tgcctttaac taaagtcttg gcggtgatgg aacttaatgg tattaaaatt gaccaagctc
    28921 ggttgattag cttggcgaag gagtggcagg ccaaattagc caaactcact caagaaattt
    28981 atcggcaagc tgggcaggag tttaatatta actcgcccaa acagctgcaa gtcattttat
    29041 ttgatcattt aaaattaagc agtaaggggc tagccaaaac caaaagtggg ccatcaaccg
    29101 atgcggccaa tctacaaaag ctaaaacagc aacacccaat aattaatcta ttgattgaat
    29161 atcgtgaatt atctaaactg cttaacacct atgctttggc tttaccaaaa ttaattaatc
    29221 ataatgatgg tcgattgcat gccaattttc aacaagcgat aacagcgacg gggcggttat
    29281 cggccagtga accgaattta caaaatattc caaccaagac ggaaattgga aaaaaattgc
    29341 gccaagcttt tattgctgat ccgggttgtc agttgattag ttttgattac tcacagattg
    29401 agttgaggat tatggctagt ctggctaatg aaccgagcat gattgctgat tttgccgccg
    29461 gtcgagacat tcacttggcc acggcagcca agattaacga tattcgtctt gatcaggtta
    29521 gcgatcagca gcgacgggca gccaaggcgg ttaattttgg tttgctatat ggtcaagggc
    29581 cgcacggttt ggcggaagca accggactca gttatagtgc ggccaaggat tttattgatc
    29641 aatattttgt tatctatcca cgcattgccg agtatatgaa caaagccatt gatcaagctc
    29701 gtcattgggg ttttgcggcg actgtttggg gccggcgtcg ttatctgccc gacttagata
    29761 gtcctaatca ggctattcgt cgtgctgccg agagaatggc cattaatcta ccgattcaag
    29821 gaacagccgc cgatatcatg aaagcagcga tgattgcagt tgacgattgg ttgtctaaga
    29881 attttaatca gcggcaagcc aggcttattt tacagattca cgacgagata ttaattgagg
    29941 cagctagcga agaggtggac aaaataatga cggcagtgcc aaaattaatg accgacgtta
    30001 ttgatttaac agtcagctta gtggttagcg ctaagactgg ttttaattgg gctgaattat
    30061 aaatatatga taaattggca agattattca gctaagcgaa ttgctgttct gggtcttggt
    30121 tcggaaaatg aagccttaat tttgtggtta gctgaacaga acgtggcgat agatattact
    30181 atttatgatc gtcgtccgct tgaagccatt gctgacaaga aagaaaaaat tatgcgtgcg
    30241 gttggaccat taacggttag ctggcgatta ggtgagcaat cttatgatag tctagaggat
    30301 tatgatgaat tgtggcgatc gccaggttgg ccgatttttt gtccgcaagt tcaaggagca
    30361 ttacaaaatg gacgaagatt aaacagcccg atgaatttat tttttgagac ttgtcctcgg
    30421 caacggatga ttggcattac cggcagcaaa ggtaagggta cgactagcag tttaattgcc
    30481 aatattttag aggcggctgg ccagccagtt ttgttgggtg gtaatattgg cgtgccaccg
    30541 ttttctttta ttaatagaat taatgatcag tcgtgggttg ttttagaatt atccagtttt
    30601 cagttagaag atttgcaata tagtccgcac attgccgtta taactaatat ttatgaagag
    30661 catttggctc cagctgatcc gcttaatcct aattatcata aaacccctga agattattgg
    30721 ttggccaagc aacagatatt tcgacagcaa aaagcgggag attggttggt tgttaattat
    30781 aaattagaaa ataaattaga taacaatcta attaatagtc aagttaaatt ttttagtgag
    30841 ttaaattggc caagtcattt ggttggacaa cacaatcaag agaatatcgc cgctgctcag
    30901 gcagtgattg aaatcattgg tttgtcggaa aaaatcagcc gacaagcagt ggctaatttt
    30961 aagggtctag aacatcggtt ggagtttgta gctaccaaag acggagttag ttattatgac
    31021 gatagttttg cgactaatcc aatcgccacc acgattgcta ttaaagcttt taatcgtcca
    31081 gttattttat tagccggcgg ggctgataag gggaataatt ttaggccgtt agcccaaaca
    31141 attgtagagc attcagttaa aacggtgatt ttgtttgatg gcactgcttc accacgaatt
    31201 aaaagtgatt tattggcgac tggttatcca gaggagatga ttttatcagc cggcagcatg
    31261 gatcaggcca tgcagctggc taagcaaaaa gccgttgacg gcgatgtggt gctattgtca
    31321 acggcttgtg ctagttttgg tatcttcaat aattataaag agagaggcaa tttatttaag
    31381 caagcggttt aggtccgacc aatttttcaa aagcctcttt ttcaatcgtt tctttaacga
    31441 ctaattcttg actgattttg tccagctcag ttcgatgttt ctgaataaaa tgttcagcta
    31501 ggttgcggcc agtctgaata aagttattta tttcttgatc aatttcttca gcaatttttt
    31561 cactatagtc gcgctgttca cgaatttctc ggcccaagaa aatcatttct tctttttgac
    31621 caaaagtccg gggtccgagc ttgtcgctca taccaaaatc agtaaccagt tttctggcca
    31681 gattggtagc tcttcgtaag tcggaagtgg cgccggtcgt aacctcgcca tagactaatt
    31741 gttcggttag ataaccgcct agcagaacgg ctatttcttc tagaaattct tgtttgcttt
    31801 gaagatgctt gtcgcgatcg ggcagtttga gggtataacc gccggcttgt ccgcgagcaa
    31861 taatagaaat tttatgaact tggtcggtat gaggcaggta atgagcaact agagcatggc
    31921 cagcctcatg ataggcggtg attaattttt ctttatcact gagaatacga ctttttcttt
    31981 ccgggcccag caagaccttt tcaatactca ttagtaactc ttcattatca ataacttttt
    32041 tatttttagt agcggccaaa atagcggctt cgtttagaac attagctaaa tcagcgccgc
    32101 taaagccagg agttcgttgg gctaccaagt gcaaatcggc atttttagtc agcggcttgt
    32161 tgtcggcatg aatttttaag atagcttcgc gatctttcag gtctggttca tctaaaataa
    32221 cctgccgatc aaaacgacct ggccgcaaca aagccgggtc aagcacatcc ggccgattag
    32281 tggctgccat aacgataaca ttagtgccaa cttcaaaacc atccatttct accaagatct
    32341 gattaagggt ctgttcgcgt tcgtcatgcg acccgcctaa gccagcgccg cgccgccggc
    32401 caacggcatc tatttcatca ataaaaacaa tacagggggc gcttttttta gcttttttaa
    32461 ataagtcacg gactcgggaa gcgccgacac caacaaacat ttcgacaaat tctgagccag
    32521 acaagctaaa gaaaggaaca ttggcctcgc cggcaacagc tctagctaag agagttttac
    32581 cagtgccggg gctacccagt aacaataccc ccttaggtat tttagcgccg aggtcggtaa
    32641 attttttagg ttgacgcaaa aattgaacga cttcttgcaa ttcttcttta gcctctttaa
    32701 cgccagcgac atccttgaaa gtgattttat ttttacgacg atcaatttct tgagcagtcg
    32761 tttgtccaaa actaatagcg cgattattag ctccctgagc tgagcgcatg acaaaataga
    32821 aaaggccgat aattaaaatt aaaggcagag ccaagggcaa tatcgtagta atcagcaggt
    32881 tgctagtggt gttgtttttg accttgatac tgatggcggc cattttttcc ggagcgactt
    32941 caaaattgcg cagtaattgg ctcaggcttt cgcctggttc tttaacggcg atttgtcgac
    33001 gattgtcttt caggttaata tttagggtat cgccgtcaat agtgatagct gtgatctgat
    33061 cattattgat ttcttgaact aaagactcaa taccaacttt ttctatctta gcggatcggc
    33121 tattatatag gctaaaaaga tagctgatgc ttaaaaagac gattaggaca agaaagaaat
    33181 ttttaagtag atttttcata gggaaataag ttttaattat tttagtatag cctaagaata
    33241 gtggtttgac aacaataaaa ccgccattag gcagttttat tgttgtattg atagtgtgtt
    33301 tattcggccg gtgtttcctt agtgtctgct tttttagtgg tttttttggc cggtgttttc
    33361 ttgggcttaa tatctgcttc agtttttggt tgtgattcag tagcagcttt agtggttttt
    33421 ttggttgttt ttttagtttc tttcggggtt gattcaattg attctttagc atcctgatcc
    33481 gctttattct ttttaaccac attagctagt cctaggcggt ggctatctgg ctctagggaa
    33541 ataatagtaa attcggcttg ggtattgggt gtataggcat cagtaatttt ttgtccagga
    33601 tttaaggata gttggctgat atgggctaga ccatgaatgt cattatccag ttcaacaaac
    33661 aagccgaaag gattaacttt taagactaaa ccggtaactt tttgaccgac agcatatttc
    33721 ttagcgacat tcttccaagg atcagcaatc agttttttgg ccgataagaa aattttggaa
    33781 ccatcaatag aaatgatttc tgccttgatg gtatcgccga tcttgtagag ttcattgata
    33841 tcgtcaattc tttgccaagc cagttcactg atatggatta agccctccag attattgtca
    33901 aaattaataa acagaccgaa accggtgatg gcggtaatgg cgccttcaac tacgctgccg
    33961 acttgatagc gggagattat atctttttgt ttgtcctgcc aggcatcttt ttcgctgaca
    34021 atcaacttct tttcttcttc atctaaggtc attactcgga cttccaattc tgagccgacc
    34081 agggttttta gtttttctaa aattttagtt ttgtcgccgc cgctaacacg aggataattc
    34141 tccggggcta attgagaaac cggcaaaaaa ccggtaattt gtcccagcga aataattaag
    34201 ccgcctttat tggcgtccaa aacgcgaacc tttaagttgg tcttattgga ataactttct
    34261 tgcaaattaa cccaagcttt ctgttcgcca gcatgacgaa aagacaattc taactcgcca
    34321 ttttcgttat cagtgtcaac aaccgtagct tcaatttcct cgccggccgg tagtttagca
    34381 taatcgctgg catcaaaata cagttcgcga ccgcggataa ttccggtcat gacgccatta
    34441 atatctaact taacttctga tttagaggct gagataattt taccggtgat agtatcgcca
    34501 acctgaggaa taaccagctg atcgttggcc agcaatttag caaattcgct cttgtcgtta
    34561 gtctgattca taaaaaataa ctaggctatt cacctagtgc tgtaattaac tttcaaccga
    34621 gatgtctccc ttggttgaag tcttttggcc acctgatacc aaaagttttt aataggtgtg
    34681 taagcataat aagttattat tcaaaaaaaa gcaagtgcag gaatcagcca atattgacaa
    34741 attttgttag ctgtgataat gtggcaaatc cctttaattc tacccatgct taattaattt
    34801 ataaaagcta aggcttaagg gtttttaagt taattaattt tatggattat tcgcggtcta
    34861 agatccgcct gtttaacaaa aaacaaacca gacaaatcag ctcgttgtta gtggcagttg
    34921 tcgcctttga attattattt tttccatggc caacttttgc tgccaatcaa gacgaacaat
    34981 cgagctcaac agctgagacg cttagtttta atttagatgc caataatatt ataccgcgcg
    35041 atttaactaa tgaccgctgg tcattacgtt taccgccaga agaagtgctc gttgaagatg
    35101 ttaaaaaagt tgaagggcaa tttagcggcc gcctaaccaa gactcggcaa gttgttttaa
    35161 ccgcctataa ttcagataag gctcagtgcg acgacagtcc ttgtattaca gctagcggct
    35221 ttaatgtctg tcagcatggt attgaagaca cagtggccgc caattttttg ccgttgggcg
    35281 ccaagattaa gattccggct ttgttcggtg accgggtttt cgttgtccgc gatcggatgg
    35341 ccaagcgcta tcaaaataga gtggatattt ggatgaaaga atatagcgca gcgattaaat
    35401 ttggcatcaa gacggctact attgagattc taccagcgga gttagctatt aataaatagt
    35461 ttttactaat aatacgaaaa acaccccatt tggggtgttt ttcttgtaaa ctaaatagaa
    35521 ttattttttg gtttttgact tcgccttagg cttagctgcg gcgatcggct ggccgacatt
    35581 gagccgtttc tgcaaccgtg attttttgcg gctggcggtg ttttttttga taacaccttt
    35641 tttaacagcc ttgtctaaag ttttgatggt ttgtttaacc atggccacgg cttcagcggt
    35701 tttggcatca atagctcggc gactaccctt aaccattttt tttatctctt ggcgcacctg
    35761 ttcattatga gcggtgcggc ttttgctttt acgcaattct tttttagctg attttaaatt
    35821 aggcataatt tgaattaacc gaataaattc ggagtatgag agcgattatt aatgtatcat
    35881 gatgatggtt ttttggcaag acggattagt ttcggccagc cagtcagcaa gagagatcct
    35941 aaaataatta ataataggct aagccattgc ggccagcgcc aaccccacag cacccaggta
    36001 gggtcttggc ggaaaaattc cataattaaa cgaatagcgc cataaccaag caagtaaacg
    36061 ccaactattt tattggccgc cacttgtcgt cggtttaatt ggtaaagcag aatagctaac
    36121 aataggcaag cgattgattc gtaaagaaat aatggctggt aatggctggc agtgatacta
    36181 ttaagcggcc gatgccaaat atcaataggg atagcccagg gcatagtgct cggctggcca
    36241 aagagttctt gattaaaaaa attaccccaa cggccaatgg cttggcccag agccacacca
    36301 ggcattaacc aaccagctaa ttgccaaaat ggccattgcc gccgccggct agttagccat
    36361 aaggttatta ggccaccgat caggccgccg tgaatagcta ggccaccttg ccaaactttc
    36421 aagatgtcta aatagttgcc agcataatca gaccagtgaa taagaatatg ataaagacga
    36481 gcaccgatta agccgccgat aataatatta aaacccaagt ccaagagatt attaagaggc
    36541 cagcgatgtt ttttcgccaa tgtccaagtt aaaaatccgg ccgctaacat ggcaataacc
    36601 atagtcaagc cgtaccaata gaagttaatt ggaccaaggg taaagatgat gggcgaaggc
    36661 gtagtagtga gccagtacat aaattatatt aatgaagata gtataaaggt ttttgggtaa
    36721 aaaaagaagc caagctctgg gtagcttggc taaagttgcg ttggagatgg gctgttgtta
    36781 gtgtgtataa cccttcccca gattttcgat gttatcagga tcccagtccc agctttagtt
    36841 aacaggcttc ccattgatga ggaaattttt ccccatatct ttggataacc tgtaaaagaa
    36901 caccgatgtt tcgcccgacg gcttacctgt cagattgata ttgtcaaatt cttccagata
    36961 atccattgcc cagcggaatc gatctgggct tgattgtgct aaactatctg ggttcggact
    37021 gttgtaaagg tcattgataa ttccggtgta aaccttgtta tcaatgccgt tgatgattat
    37081 tgtttgcttc ggcgatgttg ccagcatgat gccggcgaag atggctaggg cgagcagggt
    37141 cagtagggct atttttaggt taggcatttt ttcctccccg gaatttactt tagttttttg
    37201 aatgcccatt ttagcagggc gattaagata attatactta ttgctaaact gaccaacggt
    37261 tgaaccgctg tcagcagatt gtttagagcc tgttcaatgg tgtttggtga aatcatggcg
    37321 attatcacca gcaacactat caatgttccc agtgtccggc gaggtgtgcc gaacagaaac
    37381 tttagccatt tatccatctg ggtctcctct catttaattt ttcaagtgcc atccgatgtt
    37441 tttatgatag catattattg cgtttttgtc aatcgaaaac ggtaatgtgc acacacatat
    37501 tgcactttct ggtatcctag agacatatgt ccaaaactat gccacaatca aatcaagagg
    37561 agaagatgag atggatcaaa ccacttctct taaaagaatt gactattaaa cagatggctc
    37621 aagtctctcc ctttagcgag agaactttaa agtattggct cgctagattt agagaggccg
    37681 gcgaggccgg cctcgaaaac aaatcaaggc gtcccaaaag ccaacctaat gaattaccca
    37741 taaggattaa agagaaaatt attgagttaa gacatgaaac taaactctgt gcccaaaagc
    37801 tcaattacaa actaattaag gaagggatag cggtctcaga cagagtcatt ggtaagatta
    37861 ttaaaaccga aggattagtg agaaaatata gggttagaaa gttaaagtat aaatatatca
    37921 aagtacccct agctattggc gagttagttg agatagatat taaattcgtg cctaataaga
    37981 taaggggtag gagatattac caattcacag ccatagattg tgctagccgt tggagatatt
    38041 taaaaatata tgaggactgc ggcaatggct cagccattgc cttcgtcaag cgattaatta
    38101 aaatagcacc ctttcgggtg cgcgcgatta aaacagataa tggctcatgt tttactaatt
    38161 actatactgg ttacaaaaaa tctagtgacc catttaatcc tagactccat ccattagaca
    38221 tattcttaac atcgcgaaac atcattcatt acctcattga tccgggtaag ccggctcaaa
    38281 acggtaaagt tgaaagaagc cataggactg atcaggagat gttttacaat agacaccggt
    38341 ttaaaacagc agtagaatta aaacaagaga ttaagaaatg gaatattagt tataataatt
    38401 tagagcatat tgctctagga ggactcacac caaatgaggc gttagctagg gtgcaaaatg
    38461 tgtgtgccta aaacaaaaca gccaccgctg atggtgactg tttttatgat gaaaagttga
    38521 cagtaattat tttttgcgtg gcttaaattt gccaagcgat agagtggttt taataacggt
    38581 atcggggttg agcgagatac tatcaatacc gcaagccaat aggaattgag caaaatcagg
    38641 gaaatcgctc ggggcctggc cgcagatacc aactttggtg tgggtttttt gaccaacttc
    38701 aatcaggaac tgaattaaag atttgactga ggaatttttt tcattggaga ttccggcaat
    38761 atcaagatta ccagcgtcac gatcaatacc tagggttagt tgagtcaagt cattggaacc
    38821 gatagaaaag ccgtcaaaaa ctttggcaaa atcactggct aacaggatgt tagtcggaat
    38881 ctcggccata acataaacct ccaaaccgcc ccggccctgt ttgagaccat atttagccat
    38941 agtcttaata actaatttac cctcttcaat agtgcggcag aaaggcacca tgactttgag
    39001 gtttttaagg ccaaaatctt cgcggacttt tttcagcgct aagcattcta aagcaaaagc
    39061 tggttcaaat ttgggatcgt aataacggct ggcgccgcgc caaccaatca tcgggttgga
    39121 ctcaatcggt tcataatctt gaccgccaat caagttagca tactcattag ttttaaaatc
    39181 agacaaacgg acaataacat ccttggggta gaaggcggca gcgatagtgc cgaccccctc
    39241 ggccagttta tcaataaaga attgtttttt atcagtataa ccgacagtca gttcgtctat
    39301 ttgttttttg agtttggcat ttttgatttt tgaataatta atcagtgcca atggatgaat
    39361 acggataaaa ttattgacga taaattcttc ccgagctaag ccgacaccgt cgttgggcag
    39421 aaacgaacta gcaaaagcga tatcaggcga accgatattg agcattattt tacaagctgg
    39481 ccgaggcagt ttctgaatat cggttttctt aattgagaat ggtagcttgc cttcataaac
    39541 aaagccggta tcgccttcgg cgcagctgac ggtaacagtc tggccattag ccaatttctg
    39601 actgcctgtg ctagtgccaa ctacgcaggg aatacccagc tcacgagaaa caatagccgc
    39661 gtggcaggtg cgaccgcctt tatcagtaac gatagccgag gcgattttca tgatcggttc
    39721 ccaatcagga tcggtcattt cagtaaccaa aacctgacca ggcttaaatt tactaatatc
    39781 tttaacgctc atgatccggt tgacaatccc agcgccgaca cggttaccaa cactacggcc
    39841 ttccaccacc acggttccgc gttttttaat aatgtattct tccaagatat tatagttgcg
    39901 ctggctattg actgtttccg ggcgagcttg aacaatatat agccgcttgt catggccatc
    39961 tagtgcccat tcaatatcca tggggcgttt ataatgtttt tcaataatta aaccccattt
    40021 agctaaagat ttaacttgtt ggtcagtgat ggaatagcgt tgctggtctt taatattgac
    40081 cgctacatct ttgaccggat tgctggcatt tttagcagcg taaatcattt ttattttttt
    40141 ctcacccaga cttttgctaa taatggccat ggtcggctta aagacataaa attcgtcagg
    40201 actgactttc ccttggacaa tattttcgcc aagaccataa atagaattaa taaccacagc
    40261 gttagtaaag ccggtttcgg tatcaatggt aaacataacg ccggatgaag ctaaatcgct
    40321 gcgaaccatc ttctgaatgc cgacagagag agcgatagaa aaatgatcaa attttttatc
    40381 cacccgatag gaaatagcac gattggtaaa tagagaggct aagcattttt taacggctat
    40441 taagatgtct ttttcgccag aaacattaag atatgtttct tgttgaccag caaaagacgc
    40501 atctggcaaa tcttcggcag tcgccgatga acgaacagcc acgctgagat ttttagtttt
    40561 ataaaaacca cttaatttgc ggtaggcgat gatgatttct ttttgcaagt ctttaggtaa
    40621 ttcggccttt aaaattgctt gtctggccgc ttggccgcgc ttggctaaat caacgacatc
    40681 atgagtattc aaaccatcca gaatttttct caatttttgt tgagtgccgg tgtctttcag
    40741 aaaatgacga taagcctcgg cagttgtagc aaaaccatta ggaacgcgaa tacctgattt
    40801 ggttaatttt tgatacattt caccgagcga ggcgttttta ccaccgacaa cgggaacatc
    40861 tttaatacct aattgattaa agaatttaac atatttagtc atgttgtttg gtttatgtcc
    40921 agtcagtatg actggatagt cgttaattat aaaatagccc caaggggtat tgtaataaaa
    40981 ttataacata aaaagaggcg agctgacaaa ttgttaaaat gctagtattt agtagctagc
    41041 cttgacaaaa aggccttttt gcttaacaat ggtgtatcag ttagaatagt cgcgcaggcg
    41101 tggtgaaatt ggtagacacg catgccttag gagcatgtgg agcaatccat gtgggttcaa
    41161 gtcccgccgc ctgcactagt gaatttatgt taaattttga tccgacaatt ttacagacca
    41221 ttcagcagtc cggccgtgtt ttggtagcgg ctgatatcca atcgtcaccc agcaatgggg
    41281 cagcggccgt agctttggtt agcggcctta atcaggctgg tattcaagct gacttcgtcg
    41341 ccaatggcca gttagatgac cattggtcag ttttaggttc gtttaatcaa cccaatcaaa
    41401 gtttggccag cgaagacttt attattagtt tagatttaag tcgaactaaa gttggtcaga
    41461 ttaaatataa gattgaaggc gagcggttaa attttttaat caaacccagt aatggtcaat
    41521 ttaaagagga ggatgttgaa ttattagtcg gtcgtttgcc ctatgatttg gttattactg
    41581 ttggcgtagc cgatccagaa gtgttgggtg atatttataa taataattcg gctttatttt
    41641 atcagacacc ggttattaat attgactgtc aaaccaagaa tgaaaatttt ggccaaatta
    41701 atttagttga tttggtagcc agctcggcgg ccgaggttgt ctatgactta tggtgcgctc
    41761 tgcagtggcc gttaacaccc gatcaagcca cttgtctatt agctgccttg atttatgata
    41821 ctaagaattt tactactgct aaggctagcc cgcaggtttt attagcggcg tctaatttga
    41881 tccgtgaagg ggcgcggcga gaagtcattg ttaatcaatg gtattcagca gttgctttac
    41941 cgactttgcg tttgtggggc aaagttttat ccggattgga acaagctgat aacggattgg
    42001 tgtggtccaa attggatgtt ggcgatgaaa tggtttcgga gatagccatt ggtcaggctg
    42061 ttgaaagact attgaccggc ctgcaggacg ctaaggtggt ggctattttt tataatctta
    42121 atcaatcgcc ggagccgacc gtttctttgt cgctgttaaa gaatcaggca tcactcaaag
    42181 agattaatca gtcgctgagc caaaccggca gccaaaccgg tgtggtgatt tacgctaacg
    42241 gagcaatgaa tctccgagaa tggctacagg agtttagccc aattagtcat ggccagatga
    42301 tttctttcaa gagcaatctg ccgacatcat caacaatgga attgatttta ccgcttctaa
    42361 gccggcgctt atcagaaata aattagctgt ttgccaatgg tcgattagtc ggctatgata
    42421 tttatagata ggcgcccata gctcagtggt tagagcaccg ctcttataaa gcgggggtcg
    42481 acggttcaaa tccatctggg cgcacttggt tgacaaaatt atgggcaact tttacaataa
    42541 gagcgctttg gacggttagc tcagttggtt agagcgctac gttgacatcg tagaggtcac
    42601 tggttcaaat ccagtatcgt ccac
//
LOCUS       DAOPAI010000005        31323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_1595_length_31323_cov_4.819944, whole genome shotgun sequence.
ACCESSION   DAOPAI010000005 DAOPAI010000000
VERSION     DAOPAI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1595_length_31323_cov_4.819944"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PKV49_01335"
     CDS             <1..693
                     /locus_tag="PKV49_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22288.1"
                     /translation="ISRTNEYLSSLLAQVTYSSSFFEATVKPEYQIDTTYFGDTAKKR
                     MTNWRNTIKVKPINDSGAISVKVYHPDKQQAEKLVRAINYNLITKNNYYHGLGDKVTL
                     KVIDEPLVSGWPAKPNLPLNFGLAIILGILIGLAQIYIVSINYDNKEWPEVAMDWPSS
                     EAIQNMVKQPTEAMTEAEPTNLKPQSINRVVKNSQMANQPLAMETVDIDDGQDITEQL
                     VLGGNIDNIFAS"
     gene            816..1835
                     /locus_tag="PKV49_01340"
     CDS             816..1835
                     /locus_tag="PKV49_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPL22289.1"
                     /translation="MKRIFLALVAVILGVALFGVTYTVRVGDTLEGIVAAQGVAVEQL
                     AAVNSIDSPYIIWVGQILEIPQTPQTDRPTANSGGGGNLLLPNSLTGGSITPNSSNTM
                     SLRAFCWSKSPSAGGGAGCFWSNSASGYYRWANAKWQPVVLSPKTSFGVWGEVGDDYC
                     RVTDSNWSQTALSFKVGPVLDFYTPGYWFNSWSTKVYYSFRHAETRDGAWSKIQEDET
                     WSANLWVGVHQGAKPWLSKTSASVEYTGLIEADAVGGSNGQYSSVSAYSPVKWECSVE
                     QSLYKWGNGSLSINLGYRHIDGESNPGKDRGFFGGNLQFSWAKFVYELDGSKIQQKVE
                     VQVNF"
     gene            1907..2578
                     /locus_tag="PKV49_01345"
     CDS             1907..2578
                     /locus_tag="PKV49_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22290.1"
                     /translation="MIAQCAGRFGHPTFKAGWQKPHSAVADGFAISHANQDTIKAIGR
                     DLQGRPQRIYNGGGKTIIRETIREIPASRDQSYSAPAQPIIEQPQRATPQTGTITPGS
                     NNPGPQDLLFCIDLEPAKSGDTSKKFLFWELINGLGYNISNVVANFTGRSANMLLAPG
                     MSVDGILYNGEMLRMSVSSVSSWYAQIMGIPMPDNFRPAIRSNKSGWSSRLMTRSGDY
                     FVYCF"
     gene            2635..3663
                     /locus_tag="PKV49_01350"
     CDS             2635..3663
                     /locus_tag="PKV49_01350"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPL22291.1"
                     /translation="MEEKMKIMLLVFVMAMMLVAGLSAIGSAIYWTSPVMTETDVAMI
                     AKHDLAIIDIENLFNNPDNLREIKQINPQCRVLAYVNFFEIWQSPIPPNRPFGSKLNQ
                     TITSQFSDWLLVRDDSQLASFWPGMQPLNLSDLCPVIHGYTYTRYMQEILLENILNDR
                     VWGGLFLDNLFDRVSWLGNLDLNRDGQVDDSRTVDYHWYRGVSRFLTGIRQAKGSNFQ
                     IWGNHLSLFWKDTVNGRFLEDFPTQDWCWEEAMAIAGQLPEVVFQGKGQGMDLAFNSS
                     LLLDNAFFCLGQNMAVPDSMLVKLSSLKKLPASQRGALIFNAADHTYQRQLGEELIQV
                     RLNRQTSD"
     gene            3768..5054
                     /locus_tag="PKV49_01355"
     CDS             3768..5054
                     /locus_tag="PKV49_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPL22292.1"
                     /translation="MVQQANNPVIKSKPRRPPVSIGQLLVMIGLFLTAFSLPLLDWRF
                     YLSGFEFNISDFLILLTAVIYFISRIFSQLSGQPVRAWRWPLAWPAAIFLAIILLSSV
                     LSPWPVAALKYSLRVIVFVYLFYIALPFNALDSWPKWRWPLAGAIIAGIYSSVIGLWS
                     LRWQDLHDAFFRVQPMAIFGHWWLGSNHNLLAEFLVISNFFLLAWGRFSKNDRQRRIL
                     DIVFIISVVITLLTFSRAAWIVMIIQLVLWWLSINNRRQRIGWLLAAALLVVVITPLW
                     LKMDSLQTANYSSTENRVLLTQIAWEKVQQKPFLGWGSGQFLQLVDNNLRFRAKYGDP
                     IDSHGVLQKVAAENGIIGLIALAILFVSLVAIWWRAWLNNPKARSWLTPLIIGALGGL
                     LFQLFNTSYYKGKVWLPVALSLAAIQLLAYEKKAKP"
     gene            5032..6186
                     /locus_tag="PKV49_01360"
     CDS             5032..6186
                     /locus_tag="PKV49_01360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22293.1"
                     /translation="MKKRQSHNQPLKVVCLTPHLFIGGAERLMIDIFSRLDRQHFQPQ
                     WCCLEGDSNDSANWRRELERAGIEVVVLASDQKPKQKVGRLWRLIVIGWRLWRYLRKN
                     RPDIVQTQLFADVYRPIAWLAGVPTIISVEHNLDYDEPTWTRLAKIISRWSVNGIIAV
                     SKAVKQDIIQRYHYSSNKIEVIYNGVDLDKFFQPIARPSLVDQPQLIWRLGAIGRLTR
                     QKGFDLLIKALAKLPKAIHWQCSIAGSGPQAGYLNDLITKFGLNNQIKLVGLQTDVPK
                     FLASLDLLIVPSRWEGLGLVILEAGAAGVPVVASQIDGIVEIISQRHNGYLVEAGSVN
                     QLTKGLIDVLTAVSPDERLTWAINLQQTVKEKFSIIKTVEQYQNYYEQIS"
     gene            6170..7282
                     /locus_tag="PKV49_01365"
     CDS             6170..7282
                     /locus_tag="PKV49_01365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22294.1"
                     /translation="MNKLANQKIKIVYLLPRLFGGGAERLVVDLATHLPQDQYAVTVV
                     CLKSSSTPIWREELAAAGVELVQLNKKIKLDPTCVWRLTAWLKHNRPDILHTHLFGGD
                     FYGRLAGRLAGVRHIISTEHNINFSEGYLKRQLKSWTAPLAEKVVAVSQTVLEYAQKY
                     EGVARGQGLVIYNGVPVDKFVDHNKVSDLDKKSGQPIIIGSLGRLNQQKNWPTLIAAA
                     QYLPDNSIINIAGTGPDKQKLQTIIDSSGLANRVQLIGWQANTAEFLKKLDIFVLPSF
                     WEGLPVCLLEAGASGLPVVASDIASNREVIVDQQNGLLFNPQRPPELAEKIIKLINDP
                     ELAKELAANLRGLVEQKFSFAIMLENYQRLYRQLIN"
     gene            7289..8509
                     /locus_tag="PKV49_01370"
     CDS             7289..8509
                     /locus_tag="PKV49_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPL22295.1"
                     /translation="MKIIQINKFNYLRGGAEKYFLELSERLTQAGHEVAKFCMTDRRN
                     LEDTWSGYWLSRIDFEQPAIADKLRAPERIIYSRAAKRSLTKLIKDFQPDIIHAHNIY
                     HQLSPSVLDAAREAKVPVVMTLHDYKLICPNYQLLNHGQVCEKCLDGNFWHCFREKCF
                     KNSRSQSFLASLEAVLHKHWQTYQRGVNLFIAPSQFMADKCQAAGWPQDRFRVLINPV
                     PELPAGQRQAADYFLYAGRLSHEKGIDVLLKALAGSNYKLKIAGRGPQENALRQLVKD
                     LKLSEQVDFLGQQGREQLAKLLDGTIALTVPSIWYENMPLNILEALGRGCPVVASRIG
                     GIPEIVNHQVNGWLAEPGDCGDWTKALEQAAQADPLKFSQQAKESVNQYNWSQHFLAI
                     EAIYQQILQNRGHD"
     gene            8502..10496
                     /locus_tag="PKV49_01375"
     CDS             8502..10496
                     /locus_tag="PKV49_01375"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPL22296.1"
                     /translation="MIKRFWTILWIGLWLGTAINSAQAVNWSTDYPRLANYYLKYYGH
                     VQPADLTNLYRWDLLIIPNDYQWAFPDFLTQYKQANPNGKVLAYTYPAMAMTSNLNSL
                     FRQVDEQDLWLKNGRGEKLELWPNLYAVNINKPAWTDLNLNFWQNELDRQKWDGLMFD
                     TVENSLGRFNQNGIDLDGDYRADALASLDASWQQGLARLFKTARQYWGKDKLILINGS
                     SLPIYQPSINGRMFETFPAHWEDGGTWAASMEQYLNRLPAQNYQPSVYVINSNTNNTG
                     QQNNYQKMRFGLASTLLGDGYFSFDHGDQAHEQTWWYDEYSVKLGQPTSEPYNLLAPN
                     NNKIQAGLWRRDFDHGVAVVNSTNKRQLYSLNKEELEKIRGSQAPLINNGQRVNYLLL
                     EPNDGLILLKRDLKITNQTFYNGGFVRVFDQRGQSVRNGFFAYQANLAGGDQTILFDM
                     DGQGGLEQAINRRNEVMITRRGQIIYKWYPFSQRYRGRPSINLYAPPGKKADLVIGAG
                     SGLKPVVRVYNYQGRLLSPEWLAFDQKFRGGVNVAAGQLNNRGEMAIAVTPQGGMEPL
                     IKLYSNSGKLLAPAWLAFDRKFRGGVSTAIGDISGDGESEIVAVASIGGGPQVRIFNR
                     YGKLLGQFQAFKDEAKTGYQVMINDINGDGRGEILVGQRN"
     gene            10507..11583
                     /locus_tag="PKV49_01380"
     CDS             10507..11583
                     /locus_tag="PKV49_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22297.1"
                     /translation="MAMIKKIITIWQRRYQRFYTRGYWRIVFDVGTIILVFILLILLL
                     LIKNSAISLDKRLWSSLRVPNRPTTHYKEMPLDWQIKFRPTVIGKTTNQLVADINYTN
                     LANQPINIAYSCYYGSSQQRLPLKDIADTNFTWSDGQLTTELAAGQSGQLTVSWLWSA
                     QPAASSKEIKIICRLQAGLASQTWVKPDQEFVFKKSGAVQATAGAYFYTNDGDQVGIG
                     PLPPIVGLPTSYLISWSLENAGGDLTDMQFSAQLADNVAWQGEAGLTGGHLSYDAANR
                     KVNWRMTEWPDKAPKKQVNFYISLNPTAEMVGHIMPLIKEGEWRVNDSWSEKVWRGRL
                     PALSTNLDYDSRSKGQGEVQNWLE"
     gene            11689..13020
                     /locus_tag="PKV49_01385"
     CDS             11689..13020
                     /locus_tag="PKV49_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22298.1"
                     /translation="MSNLKKKLINLWPVWLVLAAIIIIAPFSQGRVKPYYQGDAVNYR
                     GRLLIASTNMDRLQIMEANNQRINLLAELAPDKTGYLPGDNFYNCAFNQESGALYLYA
                     VNGRYLYKYNVSNPSQPELVNKIKDNSWDWFRSVGVANGRLVTVGTKGLKIWNNDLQV
                     VDSLAVQLDNPFNYSLSRDFLLAIDNDQLKMYSLPDRQWTKSVNLTIKEKGNRQIYND
                     QNSSSIFVADDWAAKQFDFSGNLINSFRFTANNGYDIVPSTDGQKIYLSDGIGVVRLS
                     KSSLQPDKWTYVKDMGVADGWSMGLRLVNWQGRDHLIIFNNGAISVLNDQLKKVANFH
                     STENRVDSQPAFSEPLMLTLDKNRAAVNSQISLRGAGFAANENLEIKFLSNQQATSSV
                     TTDSFGRFQKILTVPAIANKTAYTANPLPTDIRVVGQTSQKTYSISFQIEN"
     gene            13055..>13332
                     /locus_tag="PKV49_01390"
     CDS             13055..>13332
                     /locus_tag="PKV49_01390"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22299.1"
                     /translation="MKIYFIGQKGIPAKSGGVEKHVEALTTNLAKQGQEVFVYTRPNY
                     TDKKLTTYQGVRLISRPSLPTKHLDAFSHTLLCSLDVLFRRADVVHFQA"
     assembly_gap    13333..13342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13343..14173
                     /locus_tag="PKV49_01395"
     CDS             <13343..14173
                     /locus_tag="PKV49_01395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPL22300.1"
                     /translation="IGPASLLWLVKLLKPKTKVIFTFHCQDYYHQKWGRLARWYLRLG
                     EKIGCRLADEVIAVSHGLVDYIAKTYGRQASYVPNGVALDGYGENSDILQALDLRPQK
                     YILAVSRLVRHKGLQHLIKAFKNIKSDYNLVIVGAAAHTDDYVAELKELAADDARIIF
                     AGEQSGDNLAQLFSQAGLFVQPSESEGLSVALLEAMAYGLPVLVSDIPENLEAVGGCG
                     WSFANKDVADLTIKLQQWLDEPESGQELAKQAQNRVNSEYNWQNITNSTLKIYQLSQA
                     "
     gene            14690..15454
                     /locus_tag="PKV49_01400"
     CDS             14690..15454
                     /locus_tag="PKV49_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22301.1"
                     /translation="MSSSQESSGKWTYRLGLSTAAYAGGSGANPFITGQPGGVIINNP
                     VSGYSWVQLINQQESGRLVVTITCWDQSDVSLNYGGNFISGQPPAQWNWAGIQGSAYF
                     VAENGGGNLHFSLTAGQLLPVGGNVAQPPGLIGDDTPATLRMTVGQDSIKIFCNLDRL
                     QNFNGNAWLEYAAVNGDHNRQPLQVSSFFSGWAETVLPLSALADNPIKIRYGHNIGDL
                     ADMTQSKYWVPADGWLEFYLLPINSGKGGWEIRPTR"
     gene            15556..15933
                     /gene="tsaB"
                     /locus_tag="PKV49_01405"
     CDS             15556..15933
                     /gene="tsaB"
                     /locus_tag="PKV49_01405"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPL22302.1"
                     /translation="MKYQLFIDTVSQPGVRVAIKQADKLIIERYWSGDRQQAEQLLPG
                     ISELLTEQQLEWSDLSAIQVANQGGSFTSLRIGVVTANALAYALGLPVTGTSGQPLQS
                     DGYQIVEPEYSGQPNITIKKSPK"
     gene            16156..16587
                     /gene="mraZ"
                     /locus_tag="PKV49_01410"
     CDS             16156..16587
                     /gene="mraZ"
                     /locus_tag="PKV49_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPL22303.1"
                     /translation="MFIGEFQHNLDEKGRLAIPAKFRADLKKGAVVTKGLDDCLFLYT
                     KAAWEELVSSKLSQLSFNKANQRAFARFMLSGASEVECDAQGRVVLPEYLRQFASLRK
                     QTVIAGLYDRLEIWDKDRWEAYRSNNDKTSGSIAESLDDLS"
     gene            16591..17538
                     /gene="rsmH"
                     /locus_tag="PKV49_01415"
     CDS             16591..17538
                     /gene="rsmH"
                     /locus_tag="PKV49_01415"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPL22304.1"
                     /translation="MDYQHQPVLLEEVLAVLQPQPGQRFIDATLGGAGYTAALAKAVG
                     PKGLVVGFDLDPLAIAAAQEKLSREKLSNVLLINDNFANLSAALTERQIAGPFNGLVL
                     DLGLSSAQLADRSRGFSFNQAGPLDMAFGPDSPLSTAEIVNHWTESKLRRIISEYGEE
                     PWAGPLAKAIVSRRSRQPFVNSQELVEIVQTTLPSKYLAKSRQHPATKLFQALRIATN
                     QELDNLSAILDQSLGLLCLNGRIAAVSFHSLEDRLVKQFFLRESRDCLCPPEFPICQC
                     GHHARLKIIYRHPVMASPAEIAANPRSRSAKLRAAQKIV"
     gene            17551..17898
                     /locus_tag="PKV49_01420"
     CDS             17551..17898
                     /locus_tag="PKV49_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22305.1"
                     /translation="MTVNNQELVSTNCSKTKKSTKRCWSWQRVNRCLWLGTAVLVLSY
                     LVVANDLAAKSFALRDCKSQLTELRQQQADLQGQVALLSSYDYLRSRVQSLGMVPVDH
                     LPYAVTDDLLAKK"
     gene            17905..20106
                     /locus_tag="PKV49_01425"
     CDS             17905..20106
                     /locus_tag="PKV49_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPL22306.1"
                     /translation="MAQSGRLISRRNRSGVVQSGKRLNSNFNIVFAAFVLVGLVIIGR
                     LFWLQLIKHNYYLTKARDQHEFNVQLAPTRGQIFVHNNSAVAGSELYPIATNRRFFTL
                     YAVPNDLQTESLAKLRQQQAETQSKNNQILSIILEPSELAEILFESFDQPMILAEIDK
                     IENDRLKQQLDDQLNQLPAASSTDELAAAKEAILKRQTELLADPFYQEMSKMRRETAI
                     KEKSEAKVKEYLNILNKGADPYEVVAKKLESAQILKIYFKVLSRQQGEAAVKPDDLSI
                     SGETVVWQPAGQAKPTTITINGLGQSGRNYRYYPEKNIGANMLGFVSLENDDDLIGRG
                     QYGLEGAFDDILMGRAGSMKGERSANKATILQGRDYQQQTNGSDLILTIDRSIQNIAC
                     SKLLATVAKHQADSGSVIIINPKTGAILAMCSAPDFDPNNFQSVKDLRQFNNQPTFEA
                     YEPGSIFKAITMAIGIDQGKVTPQTTYNDAGSVMVGKNKINNSDFKGRGVVNMVRVLQ
                     DSLNTGMVFVLNRLGIDTFRDSLKKFGFGQPTGIELDSESSGNLNNLYRKFGQEINAA
                     TASFGQGINVTTLQMAMAFGVLANEGKLMKPYLVEEIVAPDGSRIKAEPQVVEQVISP
                     QAARTVSGMLVNVVEDGHGKKAGVDGYYVAGKTGTAQVPSKDGRGYLANINIGSFAGY
                     APVEDPIFVMVVRIDHPRGVVWAESSAAPLFGEIANFILKYKQVPAQRPAK"
     gene            20115..21419
                     /gene="murF"
                     /locus_tag="PKV49_01430"
     CDS             20115..21419
                     /gene="murF"
                     /locus_tag="PKV49_01430"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPL22307.1"
                     /translation="MLSQLLRYLLKRLARAVIDKYQPQIIGITGSVGKTTTKEAVLLV
                     LAKKYRVRGNIKNYNNEIGLPLTILGLESPGRSIGGWLKVLFLAWRLIQKTDPNYPEF
                     LVLEMGIDHPGDMDYLLSIVQPNIGIITVIGQAHLEFFGNQDKIAQEKGKLIVSLPSA
                     GWAVLNADDQRVRLMTSCNNRVQIITYGFGSQADVRATDLQFNYGQAGDKTIQSSAGL
                     RFKLNYQAAAVAVVLPGIISEAVVYSVLAAIAVGISQDIDLLTAIQALGDFHSPKGRL
                     NILAGYQDMILLDDSYNASPTAVAAALDILGRWPALSAQQRVAVLGDMLELGVDGPAE
                     HRRIGQIIAQLPIGRLVLVGPLAEMIGQEARQRGWTGQIDYFTDSIAAADKIREIVPA
                     GAIVLLKGSQGSRIERITKALLLDPAAAANQLVRQDSRWLDS"
     gene            21449..21796
                     /locus_tag="PKV49_01435"
     CDS             21449..21796
                     /locus_tag="PKV49_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22308.1"
                     /translation="MYIVWRIFKEIFWFPWWWYSQGWFIWIGYWWAWLNKISRGLALA
                     VWLKNIFVPMYGQRDIVSFLISFMMRIIQIIFRLAIWLVCWVIALLAIMAWPAAPVAV
                     FYYLYILYYGQEP"
     gene            21780..24518
                     /locus_tag="PKV49_01440"
     CDS             21780..24518
                     /locus_tag="PKV49_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPL22309.1"
                     /translation="MVKNRKVNNGWLVCDSCHGAGYIEGIPCATCHGHGVGWFGASHW
                     LFVDWSSSWFAIGQKQFGRRILLILDLALYLLALSGWIVLIWQVGQASNWFIDWTQIG
                     QAAWWLKPIWPRRWFAWSLFFTLAVIYRLIRNTQTIKLPQLDIDEQLLPPADWLAVFQ
                     LPKKRLLNLINQLPAEVIFSLERALLLSASHGHSQITAEHWFQVLLADPTVKSLLFRL
                     NLDGKAIADKLNKHQTKDKLKIQSISLSIEVRSAIIQGVASAWSLGEVPLTVDFLAPL
                     IKQSQLIKEIIYDTGAKEDKFSDVANWFHINRRLAKTYLQIRDSAQFKPGSNMDRAYT
                     AVATPFLNQYSYDLTLAAKNNQLPLCVARTTELNQILEAWSAGRMGVVLVGPEGVGKK
                     SLAYGLAQLMVAEDVPVFLQDKRLVEIDLATLLGGSGPEEAQSKLAIIISEVNQAGNI
                     ILFIDGIEKIAGLSSGQEQSLDLSDILANALQRGALRCLTTVDPVNYQEHLAKRAIGA
                     NLAKIDIAEPDNQQMMTIAESWASYWERRLGVIFTYAALETAKLASQRYASDPAQPAR
                     FLSVLEASARQAAKQAAPRLVSDEVVRAYASQAFGVPMQQVGQQESELLLNLEETIHQ
                     RLIGQEEAVDMVAASLRRARVELRDISKPIANFLFLGPTGVGKTELAKTLAEVYFGDE
                     NSMIRLDMSEYQQPDSVNKMIGGNGETGYLTEAVRQKPFSLVLLDELEKAHPDILNLF
                     LQVFDDGRLTDAQGRTIDFSNTIIIATSNAGALYIREQVKAGADLSTIRQWVMDNELV
                     KVMRPELINRFDGVIVFRPLNIEDMQAIARLQLNKIGRQLADKNINFQVSDQGLIYLA
                     KAGFDPEYGARPLKRLLQDTINNTLAEHLLAKTLKRRDTVLINDQGQVEIVPGVKL"
     gene            24526..>25040
                     /locus_tag="PKV49_01445"
     CDS             24526..>25040
                     /locus_tag="PKV49_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22310.1"
                     /translation="MIKENINQPLAEKINDQEAILQIIEDVGFNPNEFVDLDHHDIAL
                     RLIEAGESYSVVSNLDKFSGLDYNDIALKIIEAGEGRLIARNLDKFSGLDHNDIALRL
                     IEAGEGGSVARNLDKFNSCLNQDIALRLIETGNGGSVASNLDKFSNLDYNDIALKLIE
                     AREGSSVADNL"
     assembly_gap    25041..25140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25141..27074
                     /locus_tag="PKV49_01450"
     CDS             <25141..27074
                     /locus_tag="PKV49_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22311.1"
                     /translation="ALKLIEAREGSSVADNLDKFSGLDHNDIAIRLIKAGEGGSVVYN
                     LGKFSGLDYNDIALRLIEAGEGYSFARNLDKISGCLNQDIALRLIEDGQRYSIIGCLD
                     KFHLSSLDFYSGMKNIYPDFFTKLESQLPNLVKYLANSAELLLNLFSYEDNLTKIEQS
                     LKGNPFLIKAIDDNPRYGIRLAMKYYQFDPTAQQEIKELFDAETKIIQDHPDVDKQSL
                     EFRRLMQNHLTEFGNNADILNELTRRGVNVEQWLNYNEQLYFTLGEQEDVKFSDKIKT
                     PIIRVKETLDRYKASITNVLTDYKAELQASFISNPEEAELRQKINELQARMANEPNEQ
                     KKQGMNQGIISLQTKIDKLKPVNAWSRIQSDIFRLRSMMDNIFKFYDLCAENEANLEN
                     IKDRASLIKEKDQLQKNIAQLKVNFIEFEAFFDTYQAKLAELIAPILGQDRADALLQE
                     SVEVVGEELHHYNTDKDTLKSIFNDREADNKLKGREMRVAIASRSTQDLYLGNYCPCC
                     ICIDSAHHGAESPIADYVTDLGIQNIVVYDEKKNVPVVVCWTFIGENSPTGEPIMVID
                     NIEANTEYSVDYGQQISDNIARFVNNYAQSSGIQTIIQGPHNNDLEVFPLQKVQNKLG
                     ELYNREGGYYLEAENKDYD"
     gene            27199..27654
                     /gene="greA"
                     /locus_tag="PKV49_01455"
     CDS             27199..27654
                     /gene="greA"
                     /locus_tag="PKV49_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPL22312.1"
                     /translation="MNEQIISRDGYQKLVEELELLSTERRREIAERIERAKELGDLSE
                     NAEYSDAKEAQAFNEGRILELSNLLKSLIVVDGQTNGKVGMGSRLIAKSGSTTKEFTI
                     VSFNEADPFSGKISNESPLGQAFLGHIAGDKVKVQTPRGEIEYQIVSVD"
     gene            27754..29427
                     /locus_tag="PKV49_01460"
     CDS             27754..29427
                     /locus_tag="PKV49_01460"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPL22313.1"
                     /translation="MISKKTRPIKTKYIFVVGGVMSGVGKGVTAASIGRILINRGYKV
                     SAIKIDPYINVDAGTMNPIEHGEVFVTEDGDETDQDIGNYERFLNGNIYKENYMTTGR
                     IYQTVIERERNLGYGGKCVQVVPHIPMEVRDRIKAAVKKTGAEIIITEIGGTVGEYEN
                     LLFLEAARIMKLQDPKSVLFVMVSYLPVPHKIGEMKTKPTQHAVRALNSAGIQPDFIV
                     ARSDTPIDELRRKKIAIHCNIHNDDVIAAPDIDSIYDVPANFEKNHLSFKILKHFGLK
                     PRCNDSAAWRMMSEKVKEAKRGQQTVKIGIVGKYFASGDFCLTDSYISVIEALKHAGV
                     ANNCRPQLGWLSAEEVEEKGVGILKGYDGIVVPQGWGRRGHEGKIKTIEYCRTHKVPY
                     FGLCYGMQMAVVEFARNVCGLKGANSLEADPKTKHPVINIMLGQAELLTKRQYGGTIR
                     LGGWPCQLVPGTHLAKVYAKKFGPETKVVSERHRHRYELNNDYREILEKGGLTIAGTS
                     PDGRIVEAIEITDHPFFIGTQFHPEYISRPLDPHPLFIEFIKACWKNKK"
     gene            29497..30954
                     /gene="lysS"
                     /locus_tag="PKV49_01465"
     CDS             29497..30954
                     /gene="lysS"
                     /locus_tag="PKV49_01465"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPL22314.1"
                     /translation="MAEKISEYQERLGRLADLRQAGIDPYPAHCRREQTNGQVLANFD
                     HYLSSGETVYLVGRLRTLREHGNLSFANLQDGTATIQLAFSKKELGDKYRQFLKIIDI
                     ADFVAVSGTVFVTHTGQNSLLVTDWQLISKALRPLPEKWHGLQDDDERLRKRYLDLLF
                     NDQLRELFVKKAKFWQVTRRFMVEHDFLEVETPTLEITTGGAEANPFRTYHQDYDTDV
                     FLRISVGELWQKRLMAAGFDKTFEIGRVFRNEGSSPDHLQEFTNLEFYWAYADYRDGM
                     RLTKELYQTLAQEVFGTTKFTSKGLDFDLAGEWPEIDYQQEILRQTGIDILAVSEQAM
                     RDKLAQLEVKYDGNNRERLTDTLWKYCRKNIAGPAFVIGHPKLISPLAKARQDNPELT
                     ERFQILIAGTEIGNGYSELNNPVDQRARFEEQQKLLKAGDNEAMMPDWEFVEMLEHGM
                     PPTCGFGFGERLFACLAGQPLREVTLFPFMKPKQQ"
     gene            30959..>31323
                     /locus_tag="PKV49_01470"
     CDS             30959..>31323
                     /locus_tag="PKV49_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22315.1"
                     /translation="MLDLKYIRENIDEVKENCHYRLADVDIDRLVSLDEQRRNLQQEL
                     DAQRSQRNAWSKQKPTPEQVADIKANNEAIKQGEDNLAKIIEQLDSLLLAVPNQTDAA
                     VIRSLNEEDNPVLEEHGQPV"
BASE COUNT         8499 a         5935 c         7582 g         9197 t
ORIGIN      
        1 atctctcgta ctaatgaata tctgagttct ttattggcgc aggtaactta ttccagctcc
       61 ttttttgaag caacggtcaa gccggagtat cagattgata ccacctattt cggcgatacc
      121 gctaaaaaac ggatgactaa ctggcgcaat actatcaaag ttaagccaat taatgattcc
      181 ggcgctatca gcgtcaaggt ttatcatccc gacaagcaac aggctgaaaa attggtgcgg
      241 gctattaatt ataatttaat caccaaaaat aattattatc acggtttggg cgataaagtg
      301 accttaaaag tgattgatga gccattagtt tctggttggc cggccaagcc caatttaccg
      361 cttaattttg gtttagctat tattttggga atattgatcg gcttagcgca aatctatatt
      421 gttagtatta attatgataa taaagaatgg ccggaagtag ccatggattg gcctagttcc
      481 gaagcgattc aaaatatggt taagcagccg acggaagcga tgactgaagc agagccaaca
      541 aatttaaagc cgcaatcaat taaccgagta gttaaaaaca gccagatggc caatcagccg
      601 ttagctatgg aaacagtaga tattgatgat ggtcaagata ttactgagca attagtgctt
      661 ggtggcaata ttgataatat tttcgctagt taagtttttc agccggtcag tcgctggccg
      721 gttgaggagc tgcaactagg tttctagggg cagttgaaca ttaacaataa aataaaaata
      781 taatgccctc acaaggaggg cgaaaggaga ttattatgaa gaggattttt ttggccctag
      841 tggccgtcat cctcggggtc gctctctttg gggtgaccta tacggtgagg gttggcgata
      901 ccctggaagg gatcgtcgca gctcaggggg tggcagttga gcaattagct gccgttaaca
      961 gtattgactc accctatatc atttgggtgg ggcagatcct ggaaatacca caaacccctc
     1021 aaaccgaccg accgaccgcc aatagcggag gtggtggcaa tttattgctg ccaaattctt
     1081 taacaggcgg ttcaatcacg cctaattcca gcaacaccat gagtcttcgg gctttttgtt
     1141 ggagtaagtc accgtcagct ggcggtgggg ctggttgttt ttggagtaat tcggctagcg
     1201 gttattaccg ctgggcgaac gccaaatggc aaccggtggt gctctcaccc aagacatctt
     1261 ttggtgtttg gggtgaggtt ggagacgatt actgtcgggt aaccgacagt aattggtcac
     1321 aaaccgccct gtcgttcaaa gttgggccag tcctggattt ttacactccg ggttattggt
     1381 tcaactcgtg gtctaccaag gtttattaca gtttccgcca tgcagaaacc cgtgatggag
     1441 cttggagtaa gatccaagag gatgagacct ggtcagctaa cctttgggtt ggtgttcatc
     1501 agggtgctaa gccatggctt agcaaaacca gtgcttccgt tgagtacact ggcctaattg
     1561 aagccgacgc tgtcggcggc agtaatggtc agtattcatc agtttcagcc tactccccag
     1621 ttaaatggga gtgctcagtc gagcagtccc tatataagtg gggaaacggc tcgttaagta
     1681 ttaatttggg ttatcgccat attgatggcg aatccaaccc agggaaagac cggggattct
     1741 ttggcggaaa tctccagttc agttgggcga agttcgtcta tgaactggat ggaagtaaaa
     1801 tacaacaaaa agtagaagtt caggtgaact tctaataaaa aaaggagcaa agatgaaaaa
     1861 aataggaaga gtcgttactt ggctgttagt gatcataata atcattatga ttgctcaatg
     1921 cgccggaaga tttggccatc ctacatttaa ggcagggtgg caaaaacccc attcagcggt
     1981 agctgatggt ttcgccatca gccatgcaaa tcaggacaca atcaaggcca taggcaggga
     2041 cctacaaggc cgtccccagc ggatttataa tggcggcggc aaaaccataa tccgtgagac
     2101 aattagggaa atccctgcta gccgggatca gagttactcc gctccggccc aacccatcat
     2161 agagcaaccc cagcgggcta caccgcagac cggcactatt acccctggca gtaacaaccc
     2221 aggtccgcag gacttattgt tctgcattga cctagagccg gccaagtccg gcgatacttc
     2281 caagaagttt ctcttctggg agttaatcaa tggtctcggc tacaacatct ccaatgttgt
     2341 agccaacttc accggccggt cggccaacat gttgttggcc ccaggaatgt ccgttgatgg
     2401 tattctctat aatggagaaa tgctcagaat gagcgtttct tctgttagtt cttggtatgc
     2461 ccaaatcatg ggcataccga tgccggacaa tttccggccg gccatccgct ccaacaaaag
     2521 cggttggtcg agtcgtttaa tgacgagaag cggggactac ttcgtctact gcttctaata
     2581 accaacttca atttgaggga ggatggcctg gaaaccgttc tccctctctt taatatggag
     2641 gaaaaaatga agataatgct tttggttttt gtgatggcca tgatgttggt tgctggactg
     2701 tcagctattg gctcagccat ttactggact agcccggtaa tgactgagac cgatgttgcc
     2761 atgattgcca agcacgactt ggcaatcatt gacatagaga atttgtttaa taacccggat
     2821 aatctccggg aaattaagca gatcaatccc cagtgtcggg ttctggctta tgtcaatttc
     2881 tttgagattt ggcagagccc gattccaccc aatcgaccat ttggcagtaa gctgaaccag
     2941 accatcacta gccaattctc agattggctg ttggtgaggg atgatagcca gttggctagt
     3001 ttctggccag ggatgcaacc gctcaatctg agcgatttgt gtccagttat ccatggctat
     3061 acctataccc gatatatgca ggagattctc ttggagaaca tcctaaatga tcgggtctgg
     3121 ggtggccttt tcctcgacaa cctgtttgac agagtgtctt ggctgggtaa cctagacctg
     3181 aaccgtgatg gtcaagtgga tgattctcgg acagtcgact atcattggta tagaggggtt
     3241 agccggtttt taaccggcat ccgtcaggcc aaaggtagta atttccagat ctggggtaac
     3301 catttatccc tgttctggaa ggatactgtc aatggccgtt ttctggaaga cttccccacc
     3361 caggactggt gttgggaaga agctatggca attgctgggc aattgccgga agtcgtcttc
     3421 cagggtaaag gccaaggcat ggatctggcg tttaatagca gcttgctgct agacaacgct
     3481 tttttctgcc tcggccagaa catggccgtg cccgactcta tgctggttaa gttgagcagc
     3541 ttaaaaaagc tgccggctag ccagcgtggg gctttaattt ttaacgccgc tgaccatact
     3601 tatcagcggc aattaggaga ggagctgatt caggtacgcc tgaatcggca aacttccgac
     3661 taacaattga cctgtcatcc cgttaagctt acgagcttga tgggatggca ggtttttttc
     3721 ttggttaaaa gtttgacttt atccatattt tatggcattt tagagctatg gttcaacagg
     3781 caaataatcc agtcatcaaa tcaaaacctc gtcgtccgcc agttagtatt ggtcaattat
     3841 tggttatgat tggcctcttt ttaacggcct tttctttgcc gctgctagat tggcgttttt
     3901 atctttctgg cttcgagttt aatattagtg attttttaat tttattaacg gcagttattt
     3961 attttattag tcgtattttt agtcaattga gtggtcagcc agtcagagct tggcgctggc
     4021 ctctggcttg gccagccgct atttttttag caattatttt attgagctcc gttcttagtc
     4081 cgtggccggt agcggctctt aaatactcgc ttagggttat tgtttttgtc tatctttttt
     4141 atatagccct gccctttaac gctttagatt cttggccgaa gtggcgttgg ccattagcag
     4201 gcgcaattat tgccggtatt tacagttcag ttattggatt gtggtcttta agatggcaag
     4261 atttgcatga tgcttttttc cgagttcagc ccatggctat ttttggtcat tggtggctag
     4321 gcagcaatca taatttattg gccgaatttt tggttattag taattttttc ttgttagctt
     4381 gggggcgctt tagcaaaaat gatcggcagc ggcgaatctt ggatattgtt tttattattt
     4441 cggttgttat tactttattg actttttctc gggccgcttg gatagtaatg atcatccaac
     4501 tagttttgtg gtggctgtcg atcaataatc gccggcagcg gataggctgg ttgctggctg
     4561 cggccttatt agtggttgtg attacgcctt tatggttaaa gatggatagt ttgcagacgg
     4621 ctaactatag ttcaactgaa aaccgtgttt tactgaccca aattgcttgg gaaaaagtcc
     4681 aacaaaaacc atttttaggt tggggtagcg gccaattttt acaattagtt gacaataact
     4741 tacgctttcg ggccaagtat ggcgatccga tagactcgca tggagttttg caaaaagtgg
     4801 cagctgagaa tggtattatt ggcctaatag ctttagctat actgtttgtt agtttagtag
     4861 ctatctggtg gcgggcttgg ttaaataatc ctaaagcgcg gtcatggcta acaccgttga
     4921 ttattggggc gttgggcggt ttgttgttcc agctgttcaa tacttcttat tataagggca
     4981 aggtttggtt gccagtggct cttagtttag ccgctattca attactagct tatgaaaaaa
     5041 aggcaaagcc ataatcagcc cctgaaggta gtttgcttga cgccgcattt atttatcggc
     5101 ggagccgagc gcctaatgat tgatattttt agtcgcttgg atcgccagca ttttcagccg
     5161 caatggtgtt gtttagaagg tgattccaat gattcggcta actggcggcg ggaactagaa
     5221 cgggccggca ttgaggtggt ggttttagcc agtgaccaaa aaccgaaaca aaaagtcggt
     5281 cgtttatggc ggctgatagt aattggttgg cgcctatggc ggtatttaag aaaaaatcgg
     5341 ccagatattg tgcagacgca gttatttgcc gatgtttatc ggccaatcgc ttggctagcc
     5401 ggtgtgccga ccattattag cgtggagcat aatttagatt atgatgagcc gacttggacc
     5461 agactagcta aaataatcag tcgctggtcg gttaacggca taattgctgt atccaaagcc
     5521 gttaaacaag acattattca gcgttatcat tattcatcta ataaaattga ggtgatttat
     5581 aatggcgttg atttagataa attttttcaa ccgattgctc ggcctagttt agtcgaccag
     5641 cctcaattga tttggcggct gggagctatc ggccgtctga cccggcagaa gggttttgat
     5701 ttattgatta aagctttagc taaactgcct aaagcaatac attggcaatg ttcaattgcc
     5761 ggtagcggcc cgcaggctgg ttatttaaat gatttgatta ctaagtttgg tttaaacaat
     5821 caaataaaat tagttggtct acaaactgat gtgccaaagt ttttagccag cctagacttg
     5881 ttaattgttc cgtcgcgctg ggaaggcctc gggctcgtta ttttagaggc tggcgccgct
     5941 ggtgtgccgg tagttgctag tcaaattgat ggcatcgtgg agataatcag ccagcggcat
     6001 aatggttatt tggtcgaggc tggcagcgtc aaccagctaa ctaagggttt aatagatgtg
     6061 ttgacagcgg tcagtcctga cgaacgctta acttgggcca tcaatttgca acagacagtt
     6121 aaagagaaat tttccataat taagactgtt gagcagtacc aaaattatta tgaacaaatt
     6181 agctaaccaa aagattaaaa ttgtttattt attgccgcgg ctttttggcg gcggagccga
     6241 acggctagtg gttgatttgg ctacgcattt accccaagat caatacgctg tgacggtggt
     6301 ctgtttaaag agttcgtcaa cgccgatttg gcgtgaagaa ttggccgccg ccggtgtgga
     6361 attggtccag cttaataaaa aaattaaact agatccaact tgtgtttggc gtctaacagc
     6421 ctggttaaaa cataatcggc cagatatttt acacactcat ttatttggcg gtgatttcta
     6481 tggtcggctg gctggtcgct tagccggcgt tcggcatatt atcagcactg agcataatat
     6541 taatttttct gaaggctatt taaagcgcca actaaaatcc tggaccgctc cgctggcgga
     6601 aaaagtggtg gcggtttcgc aaactgtttt agaatatgcc caaaaatatg agggagttgc
     6661 tcggggacaa ggtttggtga tttataacgg tgtgccagtg gataaatttg ttgatcataa
     6721 caaagtcagt gatttagata aaaaaagtgg ccagccaatt ataatcggtt ctttggggcg
     6781 cttgaaccag cagaaaaatt ggccgacttt aattgcggcc gctcaatatt taccggataa
     6841 ttccattatt aatattgccg gcaccggccc cgataaacaa aaactacaaa caataattga
     6901 ttcttcagga ctagctaatc gtgttcaatt aatcggctgg caagctaata cggctgaatt
     6961 cttaaagaag ttggatattt ttgtcttgcc gtctttttgg gaaggtttac cggtttgttt
     7021 attggaagct ggcgctagcg gtttgccagt agttgcctct gatattgcta gcaaccgaga
     7081 agtaattgtt gaccagcaaa atggtttatt gtttaatccg cagcgaccgc cagaattagc
     7141 tgaaaaaata attaaattga ttaatgatcc agagctggct aaagaattgg cggctaattt
     7201 gcgtggcctg gttgaacaaa aatttagctt cgccattatg ctggaaaatt accagcgact
     7261 ttatcgccag cttattaatt aatagtttat gaaaattatt cagattaaca aattcaatta
     7321 tttgcgaggc ggggctgaga aatatttttt ggaattatca gaacgattga cgcaagccgg
     7381 tcatgaggtg gctaaatttt gcatgactga tcgccgcaac ctggaggata cctggtcagg
     7441 gtattggctg agtcggattg actttgagca gccggcaata gccgataaat tacgagctcc
     7501 ggaacggatt atatacagtc gagcggccaa aagaagttta actaaattga ttaaagattt
     7561 tcagccggac attattcatg cccataatat ttatcatcaa ttatcgccat cagttttaga
     7621 tgcggctcgg gaggctaaag tgccggtggt gatgacattg cacgattata aattgatttg
     7681 tcctaattat caattgctta atcatggcca ggtttgcgaa aaatgtctgg atggtaattt
     7741 ttggcattgt tttcgagaaa aatgttttaa aaattcgcga tcgcaaagtt ttctagccag
     7801 tctggaggcg gttttacaca agcattggca gacctaccag cgaggagtta atttattcat
     7861 cgctcccagc caatttatgg ctgataaatg tcaggccgcc ggttggcctc aggatcgttt
     7921 tcgggtttta attaatccag tgccagaatt gccggctggc caacgccaag cggctgatta
     7981 ttttttatat gccggccgat tgtctcatga aaagggaatt gatgttctgc tcaaagcgct
     8041 ggctggcagc aattataagc tgaagattgc tggtcgtggg ccgcaagaaa atgctttgcg
     8101 ccaactagtt aaagatttaa aattgtctga gcaggttgat tttcttggtc agcaaggtcg
     8161 cgagcaattg gctaaattat tagacgggac gattgcctta actgtgccat caatttggta
     8221 tgaaaatatg ccgcttaata ttttggaagc cttgggccga ggttgcccgg tagtggccag
     8281 tcgcattggc ggcatacccg agattgttaa tcatcaagtt aacggttggt tagctgagcc
     8341 aggtgattgt ggcgattgga caaaagcttt agagcaagcg gcgcaagctg atccgttaaa
     8401 atttagccaa caagccaaag aatcagttaa ccaatataat tggagccaac attttttagc
     8461 tatagaagcg atttaccaac agattttaca aaatcgcggg catgattaaa cgtttttgga
     8521 caatcttgtg gatcggtttg tggttgggca cagccattaa ttcagcccag gccgttaatt
     8581 ggtcaaccga ttatccgcgg ctggctaatt attatttgaa gtattacggc catgttcagc
     8641 cagctgattt gaccaatttg tatcgctggg atttattgat tattcccaat gattatcaat
     8701 gggcctttcc tgatttttta acgcaataca agcaagccaa tcctaatgga aaagttttgg
     8761 cttacaccta tccggccatg gccatgacta gcaatctaaa tagtttattt agacaggttg
     8821 atgaacagga tttatggcta aaaaatggcc gaggcgagaa attggagctg tggcccaatc
     8881 tttatgcggt taatattaat aagccggcct ggactgattt gaacttaaat ttttggcaaa
     8941 atgaattgga tcgtcaaaaa tgggatggcc tgatgtttga cactgtagaa aatagcttgg
     9001 gtcgctttaa tcaaaacggt attgatttag atggtgatta ccgggctgac gcgctggcta
     9061 gcctagatgc tagttggcaa caggggttgg ctcggttatt taaaacagct cggcaatact
     9121 ggggcaaaga caagttaatt ctaattaatg gcagctcttt gccgatttat cagccgtcaa
     9181 ttaacggtcg gatgtttgaa acttttccgg cccactggga ggatggtggc acttgggcgg
     9241 cttctatgga gcagtatttg aatcgtttgc cagcccaaaa ttaccagccg tcggtttatg
     9301 tcattaacag taataccaat aataccggcc aacagaataa ttatcaaaaa atgcgttttg
     9361 gtttggcgag caccttgctc ggtgatggtt atttttcttt tgatcatggc gatcaggctc
     9421 atgagcagac ttggtggtat gatgagtatt cggttaaatt ggggcagccg acctctgaac
     9481 catataattt attggcgccc aataataata agattcaggc tggcctgtgg cgccgcgatt
     9541 ttgatcatgg cgtggccgtg gtcaattcaa ctaataaacg acagctgtat tctttaaata
     9601 aagaagaatt ggaaaagatt cgcggcagcc aagcgccgtt aatcaacaat ggccagcggg
     9661 ttaattattt actattagag cccaatgacg gcttaatttt actcaagcgg gatttaaaga
     9721 ttaccaatca gactttctat aatggcggtt ttgtcagagt ttttgatcaa cgcggccaat
     9781 cagtgcgtaa tggttttttt gcctaccaag ctaatttggc tggtggcgat caaacgattt
     9841 tgtttgacat ggacggtcaa ggcggattgg agcaggcgat taatcgtcgc aatgaagtaa
     9901 tgattactcg gcgcggtcaa attatttata aatggtaccc ctttagtcag cgatatcggg
     9961 ggcggcccag tatcaatcta tatgctccgc caggtaaaaa agctgattta gttattggcg
    10021 ccgggtcagg attgaaaccg gtagtgcgtg tttataatta tcaaggcagg ttattgagtc
    10081 cagagtggct ggcctttgat cagaagtttc gcggtggcgt taatgttgcg gctggtcagt
    10141 tgaataatcg tggcgagatg gctattgcgg ttactcctca aggcggtatg gagccgctaa
    10201 ttaagcttta ttctaattct ggcaaattat tggcgccggc ctggctagcc tttgaccgca
    10261 aatttcgtgg cggtgtcagc acggctattg gtgatattag cggcgatggc gaatcggaaa
    10321 tcgtggcagt ggctagtatt ggcggcggcc cgcaagtgcg tatctttaat cgttacggca
    10381 agctgttagg tcaattccaa gcctttaaag atgaggccaa aaccggttat caagtaatga
    10441 ttaatgatat taatggtgat ggccgcggtg aaatattagt cggtcagcgt aattaatagt
    10501 ataattatgg ctatgattaa aaaaataatt accatttggc aacggcgtta tcagcgattt
    10561 tatacccgtg gatattggcg gatagttttt gatgtcggca ctatcatttt agtctttata
    10621 ttacttattt tattgttgtt gattaagaat tcagcgatct ctttagataa acgcctctgg
    10681 tctagcttgc gggtacctaa tcgaccgaca actcattata aagagatgcc attagactgg
    10741 caaattaaat ttcgtccgac agtgattggc aaaacaacca atcaattggt ggcggatatt
    10801 aattacacta atctagccaa tcaaccgatt aatattgctt attcgtgcta ttatggcagc
    10861 tctcaacaac ggctgccgct taaggatatt gccgacacta attttacttg gtctgacggt
    10921 caattaacca ctgaattagc cgcaggccag tcaggacaac tgacagttag ttggttatgg
    10981 tcagctcagc cggcggcatc tagtaaagaa attaaaatta tttgccgtct gcaggccggt
    11041 ttagccagtc aaacatgggt taagccagat caggagtttg tttttaagaa atcgggagcg
    11101 gttcaggcca cagccggcgc ttatttttat actaatgatg gcgaccaagt cggtatcggc
    11161 cccttaccgc cgattgtcgg tttgccaacc agttatttaa ttagctggtc attggaaaat
    11221 gctggcggtg atttaacgga tatgcagttt tcagcccaac tagccgataa tgttgcttgg
    11281 caaggtgagg ctggtttgac tggcggccac cttagttatg atgccgctaa tcgtaaggtt
    11341 aattggcgga tgactgaatg gccagataaa gcgcccaaaa aacaagttaa tttttatatt
    11401 tctcttaacc caaccgctga gatggttggt catataatgc cgttgattaa agaaggcgag
    11461 tggcgggtta acgatagctg gtcagaaaaa gtttggcgcg gccgtctacc agcgttgtcc
    11521 actaacctag attatgacag tcgatccaag ggccagggcg aagttcaaaa ttggctagag
    11581 taatgaccaa ttgcgcgtaa cacctcttga ctatttaaat taaatatgtt aagatattag
    11641 gtcatcttta taaaagtggc tttgattgat ttaaatttaa tgtattttat gtctaatctt
    11701 aagaaaaaat tgattaattt atggccggtt tggctagtct tagcggccat tattatcatt
    11761 gctccgttta gtcagggacg agttaagcct tattaccaag gcgatgccgt taattatcgc
    11821 ggtcgattat tgattgcttc caccaatatg gatcgtttgc agattatgga agccaataat
    11881 cagcggatca atttattggc ggaactggct ccggataaaa ctggttatct gccgggcgat
    11941 aatttttata attgcgcctt taaccaagag agtggcgcgt tatatttata tgccgtcaac
    12001 ggtcgttatt tatataaata taatgtcagc aaccccagtc aaccggaatt agttaataaa
    12061 attaaagaca actcctggga ttggtttcgg tcggtcggtg ttgccaatgg tcgtctagtt
    12121 actgtcggca ccaagggcct gaagatttgg aataatgatt tgcaggtcgt tgatagttta
    12181 gcggtccaat tggacaatcc ttttaattat agtttgagtc gcgatttttt attagctatt
    12241 gataatgatc aattaaaaat gtatagcttg ccagatcggc agtggaccaa aagtgtcaac
    12301 ctaactatca aagaaaaggg taatcgtcag atttataatg atcaaaacag cagctctatt
    12361 tttgtggctg acgattgggc ggccaagcaa tttgatttta gcggtaattt aattaatagt
    12421 tttcgtttta cagctaataa cggttacgat atagtgccgt ccactgatgg ccaaaagatt
    12481 tatttgagcg atggtatcgg cgttgttcgt ttaagcaaga gcagcttaca accggataaa
    12541 tggacttatg ttaaggatat gggtgttgcc gatggttggt ctatgggctt gcgcttggtc
    12601 aattggcagg gccgtgatca cttaataatc tttaataatg gcgctattag tgttttgaat
    12661 gatcaactca agaaggtggc taattttcat tctactgaga atcgggtaga tagtcagccg
    12721 gcctttagcg agccgctaat gttgacttta gataagaatc gagcggccgt taacagccaa
    12781 atttctttgc gcggcgccgg ttttgccgct aatgagaatt tagaaattaa gttcttaagc
    12841 aatcagcagg cgacatcaag cgtaactacc gacagttttg gtcgttttca aaagatttta
    12901 actgtgccag ctattgctaa taagacagct tacacagcta acccattacc aactgatatt
    12961 agggttgttg ggcaaaccag tcaaaaaact tatagtatca gtttccagat tgaaaattaa
    13021 agaccaaatt aaatatttta attctatctt gtatatgaaa atttatttta tcggacaaaa
    13081 gggaataccg gctaaaagtg gcggcgtgga aaaacacgtt gaagctttga ccaccaattt
    13141 agccaaacaa ggccaagaag tctttgttta tactcggcct aattataccg ataaaaaatt
    13201 gaccacttat cagggtgttc ggttaattag ccggccgtca ttaccaacta agcacttgga
    13261 tgcttttagc catactctgt tatgtagctt agatgttttg tttcggcgcg ctgatgtggt
    13321 tcactttcaa gcnnnnnnnn nnattggtcc ggcttcattg ttgtggttag ttaagttatt
    13381 gaaacctaaa accaaagtta tttttacctt tcattgtcaa gattattacc atcaaaaatg
    13441 gggtcgattg gcgcgttggt atttacggct tggagaaaaa attggttgtc ggttggctga
    13501 tgaggtgatt gctgtctctc atggattggt tgattatatc gccaagactt acggccgcca
    13561 agccagttat gtgcccaatg gagtagctct tgatggctac ggcgagaatt ctgatattct
    13621 acaagcctta gacttacggc cgcagaaata tattttagcg gtttctcgct tggtgcgcca
    13681 taaaggctta cagcacttaa ttaaggcttt taagaatatt aaaagtgatt ataacttggt
    13741 gattgtcggc gctgccgctc acaccgatga ttatgttgct gaattaaaag aattagcggc
    13801 tgatgatgcg cgaattattt ttgccggcga acagtctggc gacaatttag cgcaattatt
    13861 tagccaggcc ggtttatttg tccagccatc tgaatcagaa ggtctgtcag tggccttgct
    13921 ggaagcaatg gcttatggct tgccggtttt agttagcgat attccggaaa atttagaagc
    13981 ggttggtggt tgcggttggt catttgccaa taaagatgtg gctgatttaa ctattaagtt
    14041 acagcagtgg ttggatgagc cggagtctgg tcaagaattg gccaaacaag cgcagaatcg
    14101 ggttaatagt gaatataatt ggcaaaatat tactaacagt acattaaaga tttatcagct
    14161 gtcccaagcc taaagtttgg ggtcagccga cggctagtta atcataataa ctcgtcggct
    14221 ggcgtaagtc agcactttaa aaaaaataaa aaacaaagcc cctacggggg cgaaggagta
    14281 aaaaatgaaa aggagagttc tctttttttt cgggctggca attctgctag tcatgtcttt
    14341 cctggtcggc tgttcggata tgccgaccat ccccacaata ccgggagata acactcccgg
    14401 catcaaacta gcccagggcg gcaaagatgt cgactctttg ctggtcaata cctgggccgt
    14461 cttctcggtt aataccactg gcagctcgcc agtgtcgtat agctgggagt tcggtgatgg
    14521 aacgtcggcg agtactacct ttgctcaaac cgagcatcgg tatgtgtcta ccgggacctt
    14581 cacggcctcg gtgcaggtca gctactccaa tggaggtagc aatacctaca cccggcaggt
    14641 gagggtttac caacccagta taccgccgtc ggatgacatt ctggtcctgt tgtcctcatc
    14701 ccaggaatcg tctgggaagt ggacgtatcg ccttgggtta agcactgccg cctatgctgg
    14761 cggatcgggt gccaacccat tcattaccgg tcaaccgggt ggggtgatca ttaataaccc
    14821 cgtatccggt tacagctggg ttcagttgat caaccagcag gagagtggcc gacttgttgt
    14881 cactatcacc tgttgggacc agagtgacgt ttcccttaac tatgggggaa atttcatcag
    14941 tggccaaccg ccagcccagt ggaactgggc tggtattcag ggaagtgctt atttcgtcgc
    15001 cgagaatggc ggcggtaatt tgcacttttc cttgaccgcc gggcaactct tgccggtcgg
    15061 cggtaatgtt gcccaaccgc cgggattaat aggtgacgat actccagcca ccctgcggat
    15121 gaccgtgggg caggattcca taaaaatttt ttgtaatcta gataggttac agaattttaa
    15181 tgggaatgcc tggctggagt atgcagcggt caacggcgat cacaatcgcc aaccgctgca
    15241 agtatcatct tttttctccg gctgggctga gacggtttta ccgttatcgg ccctcgccga
    15301 taacccaatc aaaatccgct acggtcacaa tatcggtgac ctggcggaca tgacccaatc
    15361 caaatattgg gtacccgccg atggttggct ggagttctat ctcctgccaa ttaacagcgg
    15421 caagggcggt tgggaaatcc gcccgacccg ataactccaa cttcccatag gcctggtctt
    15481 atttaatcag tctaatcgct gaaagaataa gacctgggcc tttctttttt ttgatgaatt
    15541 tcttacaata agattatgaa ataccaatta tttattgaca ccgtttctca acctggcgtc
    15601 agagtggcca tcaagcaggc ggataagtta atcattgagc gttattggtc gggtgatcgc
    15661 cagcaggccg agcaattatt accgggtatc tcggagttat taactgaaca acagctagag
    15721 tggtcggatt taagcgctat tcaagtggct aatcagggcg gcagttttac atcgctccgt
    15781 atcggtgtgg tgacggctaa tgctttggcc tatgccttgg gtttaccagt gacggggact
    15841 agtggccagc cgttgcagag cgatggttat cagattgtgg agccggaata ttccggccag
    15901 ccgaatatca ccatcaagaa gtcgccgaaa taagttattt ttggtccatt cagggctgaa
    15961 cacaaatttt ccacagctga ttttagagca ccccaagcgg ggtgtttttg gttataattt
    16021 ttttatctat aattagccat tatttttaat tgatcaaata cttgacgcct tttttaaaaa
    16081 agtctataat ggtcaatgtg gtgaaaagtg gtgaaaagtg gtgaatactt aataccgcta
    16141 cctaactccc aaacaatgtt tatcggcgaa tttcaacaca atttagacga gaaaggccgc
    16201 ttagcgattc cggctaagtt ccgggccgat ttgaaaaagg gggcagttgt taccaaaggc
    16261 ttggacgact gtctgttttt gtatactaaa gccgcttggg aggaattagt ttctagcaaa
    16321 ttgagccaat tgtcatttaa caaggctaac cagcgggcct ttgcccgttt tatgctgtct
    16381 ggcgccagcg aagtagagtg tgacgcgcag ggacgggtag tgctgcccga gtatctgcgc
    16441 caatttgcca gtctgcgcaa acagacggta atagccggtc tctacgatcg tttagaaatt
    16501 tgggacaaag accgctggga agcttaccgg tctaataatg acaagacgag cggttcaatt
    16561 gccgaatcac tggatgattt gtcgtaatct atggattacc agcatcagcc agttttatta
    16621 gaagaagttt tagcagtttt acaaccacaa ccaggacaac gttttattga tgccactttg
    16681 ggtggagccg gttataccgc ggctttagcc aaagcggttg ggccgaaagg cttagtggtc
    16741 ggatttgatc ttgatccgct agctatcgcc gccgcgcaag aaaaattgtc ccgagaaaaa
    16801 ttgagtaatg ttcttttgat taatgataat ttcgccaatt tatcagcagc tttaaccgaa
    16861 cgacagattg ctgggccgtt taatggtttg gttttagatt taggtctgtc atcagctcaa
    16921 ttagctgatc gtagccgagg tttctccttt aaccaggcgg gacctttgga tatggctttt
    16981 ggtccagata gtccgctatc aactgcggag attgttaatc attggaccga aagtaaattg
    17041 cggcgaatta tcagtgaata tggcgaagag ccctgggccg ggccgctagc taaggcgatt
    17101 gttagtcgcc gtagccgcca gccttttgtt aacagtcagg aattagttga aatagtccag
    17161 acaaccctgc catcaaaata tctagctaaa agtcgccagc acccagccac taagttgttc
    17221 caggctctgc gtatcgccac taatcaagaa ttggataatt tatcagccat tcttgatcaa
    17281 tcccttggct tgttgtgcct aaacggccgc attgccgcag ttagcttcca tagcttggaa
    17341 gatcgtctag ttaagcagtt ttttttaaga gagagtaggg actgcctctg tccacccgaa
    17401 tttcctatct gccaatgcgg ccaccacgca cgcttaaaaa taatttatcg ccatccagta
    17461 atggctagtc cagccgaaat tgcggctaac ccgcgatcac gcagcgccaa gttgcgcgct
    17521 gctcaaaaaa ttgtttaatc aacccccctt atgaccgtca ataatcaaga attagtctct
    17581 actaattgtt ctaaaaccaa gaaatcgact aaacgttgtt ggagttggca gcgggttaat
    17641 cgttgtttat ggctgggaac agcagtatta gttttgtctt atttggtggt ggccaatgat
    17701 ttggcggcta aatcatttgc tcttagagat tgcaaaagcc agttaactga attacgccag
    17761 caacaagctg acctgcaagg acaagtggct ctgctcagtt cttatgatta tttgcggagc
    17821 cgagtacaga gtctgggtat ggtgccagtc gatcacttgc cttacgcggt aaccgatgat
    17881 ttattggcta aaaaataggc agttatggct caatcaggac ggctgattag ccggcgtaac
    17941 cgttctggcg tcgttcagtc tggcaagagg cttaattcta attttaatat tgtttttgcg
    18001 gcttttgttt tagtcggcct agtgataatc ggtcggcttt tttggttgca gttaattaaa
    18061 cataattatt atttaaccaa ggcgcgcgat cagcatgaat ttaatgtcca gttagcgccg
    18121 actcgcggcc agatttttgt ccacaataat tcggcggttg ccggcagcga gctttatccg
    18181 attgctacta atcggcgatt ttttactctt tatgctgtgc ctaatgattt gcagacagag
    18241 tctttagcta aattacgcca gcaacaagcc gagacccaat ctaaaaacaa ccagattttg
    18301 tctattattt tagaaccgtc agaattggcc gaaatattat ttgagagctt tgatcagccg
    18361 atgattctgg cggaaattga taaaattgaa aatgatcgct taaagcaaca gttggatgac
    18421 caattaaatc aattaccagc cgcttccagt accgatgaat tagccgctgc caaagaagca
    18481 atcctgaagc gccaaaccga gttactggcc gatccgtttt atcaggaaat gtctaaaatg
    18541 cggcgtgaaa cagctattaa agaaaaatca gaggctaaag tcaaggagta cttaaatata
    18601 ttaaataagg gcgctgatcc ttacgaagta gtagccaaaa aattggagag tgcacaaatt
    18661 ttaaagattt attttaaagt cttaagtcga caacagggcg aagccgccgt taagccggac
    18721 gatttatcta ttagcggcga gacagttgtt tggcagccgg ccggccaagc taaaccgacg
    18781 actataacga tcaatggttt ggggcagagt ggccgtaatt atcgttatta tccggaaaag
    18841 aatattggcg ccaatatgct tggttttgtg tcgctggaga atgatgatga cttaattggc
    18901 cgagggcaat atggcttaga gggagctttt gacgatattt taatgggtcg ggctgggtca
    18961 atgaagggtg agcgtagcgc taataaagct actattttac aaggtcgtga ttatcaacag
    19021 cagactaatg gctctgattt aattttgacg attgatcgat ctattcaaaa tattgcttgt
    19081 agcaaattac tggcgactgt ggctaaacac caggctgatt ccggcagtgt tattattatt
    19141 aatcccaaga ctggagctat cttggcgatg tgttcagcgc ccgattttga tccgaataat
    19201 tttcaatcgg tcaaagattt gcggcaattc aataaccagc caacttttga agcctatgaa
    19261 ccagggtcga tctttaaggc gattactatg gctattggca tagatcaggg taaggtgaca
    19321 ccgcaaacta cctataatga tgccggttca gttatggtcg gcaagaataa gattaataat
    19381 tctgatttta aaggacgggg agtcgttaat atggttaggg ttttacaaga ttctttaaat
    19441 actggcatgg tttttgtgct taatcgtctt ggaattgaca ctttccgcga tagcttaaaa
    19501 aaatttggtt ttggccaacc gactggtatt gagttagatt cagaatcatc cggtaattta
    19561 aataatttat atcgtaaatt cggtcaagaa attaatgcgg ctactgcttc ttttggtcaa
    19621 ggtattaatg tgactactct gcaaatggcc atggctttcg gcgttctagc caatgaaggc
    19681 aaattaatga agccgtattt ggtggaggaa attgtagcgc cagatggcag ccgaatcaaa
    19741 gccgagcctc aagtggtgga acaggtaatc agcccgcagg cagcgcggac cgttagcggt
    19801 atgttagtta atgtagtgga agatggtcat ggtaaaaaag ccggcgttga cggttattat
    19861 gtagcgggta agaccggcac cgctcaagtg cccagcaaag atggtcgcgg ctatttagct
    19921 aatattaata tcggttcatt tgccggctat gctccagtgg aagacccgat ttttgtgatg
    19981 gtggttcgga ttgatcatcc acgcggcgtt gtctgggccg agagttcagc ggcgccgtta
    20041 tttggcgaaa ttgccaattt tattctaaaa tataaacagg tgccggcgca gcgtccggct
    20101 aaataattgt ttttatgctt agtcagttgc ttcgctattt gctaaaaaga ttagctcgag
    20161 cggtgattga taaatatcag ccacagatta ttggtattac cggcagcgtc ggcaaaacta
    20221 cgactaagga agcggtattg ctggtgctag ctaaaaaata tcgcgttcgt ggcaatatta
    20281 aaaattataa taatgagatt ggcctgccat taaccatttt agggttagaa tcgccaggtc
    20341 gctcaatcgg tggctggttg aaagttttat ttttagcttg gcgattgatt caaaaaaccg
    20401 atcctaatta tccggaattt ttagttttag aaatgggaat tgatcatccg ggtgatatgg
    20461 attatttatt atccatcgtt cagcccaata ttggtattat taccgttatt ggccaagctc
    20521 atttggaatt tttcggtaat caagataaaa ttgctcagga gaaagggaag ctcattgtta
    20581 gcctgccgtc agccggttgg gcggtgctta atgcggacga tcagcgggtt cgtttaatga
    20641 ctagttgtaa taatcgggtc cagataataa cctatggctt tggatcacaa gctgatgtca
    20701 gagcaacgga cctgcaattt aattatggtc aagccggcga caagacaatt caatcatcag
    20761 ctggcttgcg ttttaaattg aattatcaag ccgccgctgt agcggtagtt ctgccgggaa
    20821 taattagtga ggcggtggtt tattcggttt tggcggcgat tgccgtcggc attagtcaag
    20881 acattgattt attaaccgct attcaagctc tcggcgattt tcattcgcct aaaggccggc
    20941 tcaacatttt agctggttat caagacatga ttttattaga tgattcatat aatgcttcgc
    21001 cgacagccgt agcggcagcc ttagatattc ttggtcgctg gccagcgttg tcagcccagc
    21061 agcgggtagc tgttttgggt gatatgctag aattaggggt tgacggccca gctgagcatc
    21121 gtcgcatcgg gcagattatc gctcaattac cgattggccg tttggttttg gttggcccac
    21181 tggctgagat gattggtcag gaagctagac agcgtggctg gactggtcag attgattatt
    21241 ttactgattc aatagccgcc gctgataaaa tcagagagat tgttccagcc ggagcgattg
    21301 ttttattaaa aggttcgcag gggtctcgca tcgaacgaat aactaaagca ttattattgg
    21361 atccagctgc agccgctaat cagctggtcc gccaagatag ccgctggtta gatagctaag
    21421 acaggtttat tttgctatac tatttaatat gtatattgtt tggcggattt ttaaagaaat
    21481 attctggttt ccttggtggt ggtattctca aggatggttt atttggattg gctattggtg
    21541 ggcgtggtta aataagatta gccgcggttt ggctttggcg gtttggttga aaaatatttt
    21601 tgtgccaatg tatggccagc gcgatattgt cagcttttta atcagtttca tgatgaggat
    21661 catccagatt atcttccgtt tagccatctg gctggtctgt tgggtgatcg ctttgttggc
    21721 cattatggct tggccggcgg ctccagtagc ggtcttctat tatttgtata tattatatta
    21781 tggtcaagaa ccgtaaggtt aataatggct ggttggtctg tgattcttgt cacggtgccg
    21841 gttatattga gggcatacct tgcgcgactt gccacggtca cggcgttggc tggtttggtg
    21901 ctagccattg gctgtttgtt gattggtcgt cttcgtggtt cgctattggg caaaaacagt
    21961 ttggtcggcg gatattattg attttagatt tagcgctgta tttattagca ttaagcggtt
    22021 ggattgtttt aatttggcag gttggccaag ctagcaactg gtttattgat tggacacaaa
    22081 tcggacaggc ggcttggtgg ctaaagccaa tttggcccag acgctggttc gcttggtcgt
    22141 tattttttac tttagcggta atttatcggt taattcgtaa tacacaaaca attaaattgc
    22201 cgcagttgga tattgacgag caattattgc ctccagccga ttggctggct gtttttcaat
    22261 tacctaaaaa acgtttatta aatttaatta atcaattacc agcggaagtt atcttcagtc
    22321 tagaacgagc actgttgttg tcagctagcc atggccatag ccagattact gccgaacatt
    22381 ggtttcaggt tctcttggct gatccaacgg ttaaatcatt attatttagg cttaatttag
    22441 atggcaaggc gattgccgat aaactgaaca agcatcaaac taaagataag cttaaaattc
    22501 agtcgattag tttatcaatt gaagttaggt cagccataat tcagggggtg gctagcgctt
    22561 ggtctttggg tgaggtgccg ctgacggttg attttttggc accgttgatt aaacaaagtc
    22621 aactaattaa agaaattatt tatgatactg gcgccaaaga ggataagttc agtgatgtgg
    22681 ctaactggtt tcatattaac cgtcgcttag ctaaaactta tttacagatt agagattcgg
    22741 cgcaattcaa acccggttcc aatatggatc gggcttatac ggccgtggcc acaccgttct
    22801 taaatcaata ttcttatgat ttgactttag cggccaagaa taaccaatta ccactctgcg
    22861 tagcgcggac aactgagctt aatcaaattt tagaagcttg gtcagccggc cgaatgggtg
    22921 tggttctggt cggtccggag ggggtgggta agaaatcatt agcctacggt ttagcccaat
    22981 taatggtagc tgaagatgtg ccggtttttt tacaagataa acgattggtt gagattgatc
    23041 tggcaacctt attaggtggg tctggtccag aagaagctca gagtaagtta gctataatta
    23101 tttccgaagt taatcaggcg ggtaatataa ttttatttat tgacgggatt gaaaaaattg
    23161 ccggcctgtc cagcggtcaa gaacaaagtt tggatttgtc agatattttg gctaacgccc
    23221 tgcagcgcgg ggccttacgg tgtttaacta ccgttgaccc agttaattac caagaacatt
    23281 tagccaaacg agccattggc gctaatttgg ccaaaattga tattgctgaa ccagacaatc
    23341 aacagatgat gaccattgcg gaaagttggg cgagttattg ggaaagacgg ttaggagtta
    23401 tttttaccta tgccgcttta gaaacggcta aattagccag ccagcgctat gcttcagacc
    23461 cggcacaacc agctcgtttt ttaagtgttc tggaagcatc agcccgacaa gctgctaaac
    23521 aggctgcgcc acgactcgtt agtgatgaag tagttcgagc ttatgctagc caagcctttg
    23581 gtgtgccgat gcaacaggtt ggtcagcaag aaagcgagct attattaaat ttagaagaaa
    23641 caattcatca gcgtttaatt ggtcaagaag aggcggttga tatggtggcg gccagcttgc
    23701 gtcgggctcg ggtggaattg cgggatatta gtaaaccgat tgctaacttt ttatttttag
    23761 gtccgaccgg tgtcggtaaa actgagttag ctaaaacatt ggctgaagtt tattttggtg
    23821 atgaaaattc aatgattcgg ctggatatga gtgaatatca acaaccagat agtgttaata
    23881 agatgatcgg cggcaatggt gaaactggct atctaactga ggcggtgcgt caaaaaccat
    23941 ttagtttggt tttgttagac gaattggaaa aagctcatcc tgatattttg aatttatttt
    24001 tgcaagtttt tgatgatggc cgcctaactg atgctcaagg ccgaacgatt gatttcagta
    24061 ataccattat tattgccacc tctaatgctg gagcgttgta tattcgtgaa caagtcaagg
    24121 ctggggctga tcttagtact attcgccaat gggtaatgga taatgaatta gttaaagtaa
    24181 tgcggccgga attgattaat cgttttgacg gcgtgattgt ttttagaccg cttaatattg
    24241 aagatatgca agcaattgcc cgattgcagt taaataaaat cggccgtcaa ttggccgata
    24301 agaatattaa ttttcaggtt agcgatcagg gtttaattta tttggctaaa gccggctttg
    24361 atccggaata tggcgctcgg ccgctgaagc gtttactgca agataccatc aataacactt
    24421 tggccgaaca tcttttagcg aagactttaa aacgccgtga cactgtttta attaatgatc
    24481 aaggccaagt cgagatagtt cccggagtta aattgtagtt gatttatgat taaagaaaat
    24541 attaatcaac cattggcgga aaaaattaat gaccaagaag cgattttgca gattattgaa
    24601 gatgttggtt ttaatcccaa tgaattcgtt gatttggacc accacgacat agccctcaga
    24661 cttattgaag ctggagaaag ttattcagtt gtcagcaacc tagacaaatt tagcggtctg
    24721 gattataacg acatagctct taaaataatt gaagccggag aaggtcgttt aatcgcccgt
    24781 aacctagaca aatttagcgg cttagatcat aacgacatag ccttgagact tattgaggct
    24841 ggagaaggtg gttcagtcgc ccgtaactta gataaattta acagttgttt gaatcaagac
    24901 atagccttaa ggcttattga gactggcaac ggtggttcag tcgccagtaa cctagataaa
    24961 tttagcaact tggactataa cgatatagct ttaaaactta ttgaggctag agaaggtagt
    25021 tcagttgctg ataatctaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 tagctttaaa acttattgag gctagagaag gtagttcagt tgctgataat ctagataaat
    25201 ttagtggtct ggatcataac gatatagcca tcaggcttat taaagctgga gaaggtggtt
    25261 cagtcgttta taacttaggc aaatttagcg gcttggacta taacgatata gccttgaggc
    25321 ttattgaggc tggagaaggt tattcattcg cccgcaattt agataaaatt agcggttgtt
    25381 tgaatcaaga tatagcttta agacttattg aggacggaca acgttattca ataattggtt
    25441 gtttagacaa atttcactta tcttctttag atttttattc tgggatgaaa aatatttatc
    25501 cagatttttt tacaaagcta gagagtcaat taccaaattt agtgaagtat ctagctaatt
    25561 ccgcagaatt attgcttaat ctctttagct atgaagacaa cttaacaaag attgagcaga
    25621 gccttaaggg taatccattt ttaattaaag ctattgacga taatcctcgc tatggtattc
    25681 gattggccat gaaatattac caatttgatc caactgctca acaagagatt aaggagctat
    25741 ttgatgcgga aacaaagata attcaagatc atcccgatgt agataaacaa tcgttagaat
    25801 ttaggcgttt aatgcaaaac catttaacgg agtttgggaa caatgcggat attttaaatg
    25861 aattaacaag gcgcggagtt aatgttgagc aatggctcaa ttataatgaa cagctttatt
    25921 ttactttggg cgagcaagaa gatgttaaat tctctgacaa aataaaaaca ccaataattc
    25981 gcgttaaaga aactttggat cggtacaaag cgtcaattac caatgtgctg actgattaca
    26041 aggctgaatt acaagcttcg tttatttcta atccggaaga agccgaattg cgtcagaaga
    26101 ttaatgaatt acaagctaga atggcgaatg aacctaacga acaaaaaaaa cagggcatga
    26161 atcaggggat tattagtttg cagactaaaa ttgataagtt aaaaccagtc aatgcttgga
    26221 gccgaattca aagcgatatc tttaggttac gatcaatgat ggacaatatc tttaaatttt
    26281 atgatctttg cgccgaaaat gaggctaatt tggaaaatat taaagatcgc gccagcttaa
    26341 ttaaagaaaa agatcaatta caaaaaaaca tagctcaatt aaaggttaat tttattgaat
    26401 ttgaagcatt ttttgatacc tatcaggcta aattggcgga attaattgct ccgattttag
    26461 gccaagatcg cgccgatgct ctattgcagg aaagcgttga ggtggttggt gaagagttgc
    26521 atcattataa tactgacaag gacactctta aaagtatttt taatgaccga gaagcggata
    26581 ataaattaaa gggtcgagaa atgagagtag cgattgcttc tcgcagcacg caagatcttt
    26641 atttaggtaa ttattgtcca tgctgtattt gtattgatag cgcccatcat ggcgctgagt
    26701 cgccaattgc cgattatgtg actgatttag gcatacaaaa tattgtagtt tatgatgaaa
    26761 aaaagaatgt tccagtagtt gtttgctgga catttatcgg cgaaaacagc ccgaccggtg
    26821 agccgattat ggttattgat aatattgagg ccaataccga gtattccgta gattacggtc
    26881 agcaaattag cgataatatt gctcgttttg tgaataatta tgctcaatct tctggcatcc
    26941 aaacaattat tcaaggtccc cataataatg atttagaagt tttcccgctc caaaaagtcc
    27001 aaaacaaatt aggagaactt tataacagag aaggtggata ttatcttgag gctgagaata
    27061 aagattacga ttaaggttgg gggttggcta gataattaaa agaacacttg ccaaattttg
    27121 gcttttagta tataatgggc gttattgttt ttggcgcgaa gtattatttc gcgtctttta
    27181 ttaaaagtgt ttagaattat gaatgaacaa atcatttccc gtgatggtta tcaaaaatta
    27241 gttgaagaat tagagctatt aagcaccgaa cggcgccgag aaattgccga gcggattgaa
    27301 cgggccaaag aattaggtga tttaagcgag aacgctgaat atagcgatgc taaagaagcg
    27361 caggctttta atgaaggccg gattttggaa ttgtccaatt tattaaaaag tttaattgtg
    27421 gttgatggcc agactaatgg caaagtgggt atgggttcgc ggttaatagc taagagcggg
    27481 tcaactacta aagaatttac tattgtgagt tttaatgagg ccgatccatt cagcggtaag
    27541 atttctaatg aatcgcctct gggtcaagcc tttttgggtc atattgccgg cgataaagtt
    27601 aaggttcaga cgccgcgcgg cgagattgaa tatcagattg tcagcgttga ttaattttta
    27661 ggatcagctt attttataac cgcttggtca gactggctgg gcggttttgt ttttgctata
    27721 atcaaactaa ggattattat ttaattttgc gatatgattt caaagaaaac tcgccctatt
    27781 aagacgaaat atatttttgt agttggcggc gtaatgtctg gcgttggcaa gggggttacg
    27841 gcggcctcta tcggtcgcat tttgattaat cgtggttata aggtgagcgc tattaagatt
    27901 gatccttata ttaatgttga cgctggcacg atgaatccga ttgaacacgg cgaagtcttt
    27961 gtgactgagg acggcgacga aaccgatcag gacattggca attatgaacg atttttgaat
    28021 ggtaatatct ataaagaaaa ctacatgacc actggtcgaa tttaccagac ggttattgag
    28081 cgtgaacgca atttgggtta tggtggcaaa tgtgttcaag tagtgcccca tattccgatg
    28141 gaagtgcgcg atcggatcaa ggcggcggtc aaaaaaactg gcgcggaaat tataattact
    28201 gaaatcggcg gcacagttgg cgaatatgag aacttgctct ttttggaggc ggctcggatt
    28261 atgaaattac aggaccctaa gagtgttttg tttgtaatgg tcagctattt accagtaccc
    28321 cataaaattg gtgagatgaa aaccaaacca acgcagcacg cagtgcgggc gcttaattcg
    28381 gccggcattc agcctgattt tattgtcgcc cgctcagata cgcccattga tgaactgcgt
    28441 cgtaaaaaga ttgctatcca ttgcaatatt cataatgatg atgtgattgc cgccccggat
    28501 attgactcaa tctatgatgt gccggccaat tttgaaaaga atcatttgtc atttaaaata
    28561 ttaaaacatt ttggcctcaa gcccaggtgc aatgactcgg cggcttggcg aatgatgtca
    28621 gaaaaagtca aggaagccaa gcgcggtcag cagacggtta aaatcggtat agtcggcaag
    28681 tatttcgctt caggcgattt ctgtttaact gattcatata ttagtgttat tgaagccttg
    28741 aaacacgctg gagtagctaa taattgtcgg ccgcagttag gatggctgag tgccgaagag
    28801 gttgaagaaa aaggagtagg cattctcaag ggttatgatg gtattgttgt gccgcagggc
    28861 tggggtcgtc gtggccatga gggtaagatt aagactattg aatattgtcg cactcataag
    28921 gtaccttact tcggtttgtg ttatggtatg caaatggcgg tggttgagtt tgctcgtaat
    28981 gtttgcggcc taaaaggcgc taatagctta gaggctgatc cgaagactaa gcatccggtt
    29041 attaatatta tgctcgggca agctgaatta ttaactaaaa gacaatacgg cggcactatc
    29101 cgcctcggtg gctggccgtg ccagttagtg cctggaacgc atctggctaa ggtttatgcc
    29161 aagaaatttg gccctgagac taaggtggtt agtgaacgtc accgccatcg ctatgaactt
    29221 aataatgatt atcgggaaat attagagaaa ggcggcttga ctattgctgg cacttcgccg
    29281 gacggacgga ttgtggaggc aattgagatt acggatcatc cgttttttat cgggacgcaa
    29341 tttcatccgg agtatatttc acgaccgcta gatccgcatc ctttatttat tgagttcatt
    29401 aaagcttgct ggaagaataa aaaataaatt tttctttact tcccactcta aattagctac
    29461 aatataacca gtttaattac tggttatatt gtttttatgg ctgaaaaaat tagcgaatat
    29521 caggaacgct tggggcgatt ggccgattta cgccaagccg ggattgatcc ttatccggct
    29581 cattgtcgtc gcgaacaaac caatggtcaa gttttggcca attttgatca ctatttatct
    29641 agcggcgaga ccgtttactt agtcggccgg ctacgcacct tacgtgagca cggtaatcta
    29701 agttttgcta atttgcaaga cggcacggct actattcaat tggcttttag taaaaaagaa
    29761 ttaggggata aatataggca atttttaaaa attatagata ttgctgattt tgtggctgtc
    29821 tcgggcacgg tttttgtcac ccacaccggt caaaatagtc tactggtgac cgattggcaa
    29881 ttaattagca aagctttgcg gccgctacca gagaaatggc acggtctgca agatgatgat
    29941 gagcgtttgc gcaagcgtta tttggattta ttatttaatg atcagttgcg cgaactattt
    30001 gtcaaaaaag ctaaattttg gcaggtgacc cgtcgtttta tggtggagca cgactttttg
    30061 gaagtggaaa caccaaccct agaaataaca actggcgggg ctgaggctaa tccttttagg
    30121 acttatcatc aagattacga tactgatgtt tttttgcgta tttcagtcgg cgaattatgg
    30181 cagaaacgct taatggctgc cggttttgac aagacttttg aaattggtcg ggtttttcgc
    30241 aatgagggca gtagccctga tcatctgcag gaattcacta atttggaatt ttattgggcc
    30301 tatgctgatt accgcgatgg catgagatta accaaagagc tttaccaaac actagctcaa
    30361 gaagtttttg gcaccaccaa gttcactagt aaagggttag attttgattt agccggcgag
    30421 tggccggaga ttgattatca gcaggagatt ttgcgccaga cgggcattga tattttagcg
    30481 gttagcgagc aggcgatgcg tgataaatta gcccaattag aagtaaagta tgatggtaat
    30541 aatcgcgaac ggttaactga tactttgtgg aagtattgcc gcaaaaacat tgccggcccg
    30601 gcttttgtta tcggtcatcc caaattgatt tcaccattag ctaaagctcg ccaggataat
    30661 cctgagttga ccgagcgttt tcagattctg attgctggta ccgaaatcgg caatggttat
    30721 tccgagctta ataatccggt tgatcagcgg gcgcgttttg aggagcagca gaaactgctg
    30781 aaagccggtg ataatgaggc gatgatgccg gattgggaat ttgtagaaat gctagagcac
    30841 ggtatgccgc cgacatgtgg tttcggtttt ggcgagcgtt tatttgcttg cctggctggt
    30901 cagccgcttc gagaagttac cttatttcct tttatgaaac caaagcaaca ataattttat
    30961 gttagatcta aaatatatcc gtgagaatat tgatgaagtt aaagaaaatt gccactatcg
    31021 tttggctgat gttgatattg accgtttagt cagccttgat gagcagcggc gcaatctaca
    31081 acaggaattg gacgctcagc gttcccagcg caatgcttgg tctaaacaaa agccaacgcc
    31141 ggaacaggtg gctgatatca aggctaataa tgaagcaatt aaacaaggcg aagataatct
    31201 agcgaaaatt attgagcaac tagatagttt attattggct gtgcccaatc agactgatgc
    31261 ggcagtaatt cgcagtttaa atgaagagga taatccggtt ctcgaagaac acggtcaacc
    31321 ggt
//
LOCUS       DAOPAI010000006        27018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_1942_length_27018_cov_6.048585, whole genome shotgun sequence.
ACCESSION   DAOPAI010000006 DAOPAI010000000
VERSION     DAOPAI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1942_length_27018_cov_6.048585"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="PKV49_01475"
     CDS             <1..1780
                     /locus_tag="PKV49_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22316.1"
                     /translation="MSATPKIKPDPRDLARGTAAYVFVEPKDVIDEGMIKKELIINEK
                     IDEIIDDEMDSQKVVLETAYKKRLELKKFFEAEKSNINPLVLVQIPVAEAGEDKIRAV
                     KKFLADKDITERKNGQGNGKLAIWLSEQKSETLDWIAEPDNEIEFLIFKQAIDTGWDC
                     PRAHILVKFRESHSETFEIQTVGRILRMPEQKHYASEDLNRGYIYTNVQSIIVKKEEY
                     NPNIIKHLKATRKDVYRPIKLTSYYKSRADYGDITSSFTPVFEKTACEEFGLKGDHTF
                     FAQNVKKVEDKGVVLDIKKYQQEIIANARIKGKTFDEIEGKINSADRARLIIAGNDLQ
                     ALFEQIIKNNLGSFKNIKRSVPTVKTAIYSWFKKYLGSKEWSEEIILVQMIFVHNGNR
                     KKFEKILASAIEKYKAVREKEILKRVEESEQFYDFEIKKESSFNQYIDELMSYKKYVY
                     DPCYLSVNRSIPEKNFEKFLVENDDKIVWWWKNGENKQDYFGIKYEYPVGVIHTFYPD
                     YLAQFTDGRIGIFETKDTGDRDGGTYTKAKAEKLQEYIKNQKSQKLFGGIVIEKSDGW
                     KINQKPLYNWSKCERNDWSDWDKLKI"
     gene            2107..2874
                     /locus_tag="PKV49_01480"
     CDS             2107..2874
                     /locus_tag="PKV49_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22317.1"
                     /translation="MKRLLLIIILIIVIIGVFLLWYSPVIFQEGHPWPQLKGIIQLNF
                     TNKDLVKLSSEKNKWLTKSKNGAAVIKSYMKNQGYDFTEQMGSAYFFQSATGAQIIAT
                     HRYYSRFYSLWTMIETINNTDDAEQLSIAEELADCLPKSDTASRDQCTTLLATIKNFT
                     DCANAGFTIIQSDPPQCSTPDGRSFTDNTISSWEVAVASLNNCEVKSIFQAHSKLVAL
                     KLKNGNTILTYEPQIDDVMKIAVDLTSKCGAIHMATE"
     gene            2878..3009
                     /locus_tag="PKV49_01485"
     CDS             2878..3009
                     /locus_tag="PKV49_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22318.1"
                     /translation="MEIRDTVDGKIYGKSYNDIISQTARLGCQPIINNNASMTTRYE"
     gene            3002..3475
                     /locus_tag="PKV49_01490"
     CDS             3002..3475
                     /locus_tag="PKV49_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPL22319.1"
                     /translation="MNKIDWPKLIGSLVITLGAGWLGSSLTRPNNSWYANINRTVLTP
                     PNWLFAPVWTILFCLMAAALYIIWSGRNKKKTRKKAVQLYFGQLAANIAWSGIFFGLQ
                     SISLAFIEIVFLLILITYCLVAFSRINRAAGYLMLPYFLWVCFATVLNLSLWLIN"
     gene            complement(3542..4666)
                     /gene="rseP"
                     /locus_tag="PKV49_01495"
     CDS             complement(3542..4666)
                     /gene="rseP"
                     /locus_tag="PKV49_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPL22320.1"
                     /translation="MTVVIFLAVLSVLVVVHELGHFLAARWNGVGVEEFGLGFPPRLI
                     GWFKNGRGHWRTIGWRPPRHRHTIFSLNWIPLGGFVKLHGEDGIDRPNDPQSFSNKKA
                     GQRILVVIAGVVMNIVLAAIIFIIGLSVGWPQATNNLGPQARVANQQIAIIEVIPDSP
                     ANQAGLQAGDLITSINQQTIASINQLRQLNQESAGQPLNYQIKRQETELDIQVKPQMM
                     DGNNSQYTIGAALAESGLVSYAWPRAIWEGWRLTALSLKDIAVGFWQLLNGLFHGHNM
                     ADQVAGPVGIAVLTGQISSLGFIYLLQFVALLSLNLAFINILPLPALDGGRLLFIAIE
                     KITGHRTKAQIEALVHNIGFILLLSLIILITFQDVSRLLR"
     gene            complement(4668..5222)
                     /locus_tag="PKV49_01500"
     CDS             complement(4668..5222)
                     /locus_tag="PKV49_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HPL22321.1"
                     /translation="MNQFIDNQSADFQKAIDFFIKDIASLRTGRAHPGLLDNISIEAY
                     GSFSPINAMANISVQDARCLVVTPWDRSVLKNIEKSLTAADLGMGIINEGDKLRLTMP
                     ALNEENRRELVRRLNEKLEESRIVLRQVREEVKNKIEAAFSAKEINEDNKFRFIKELD
                     DFLGKKNEELKALRDKKEVEIMTI"
     gene            complement(5250..7169)
                     /locus_tag="PKV49_01505"
     CDS             complement(5250..7169)
                     /locus_tag="PKV49_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22322.1"
                     /translation="MTRTPKKPAIDYFEQMIKVGQPIRKKASRSIDRAEAKEQEVDDS
                     LLEIYQNDQGQIVDVSRFTVRRRRWWVKVLLFMLYSLILVLAGWLGYNYYTGYRAKAD
                     LLTINIETDKNLTAGQEFTYTINYRNNGSVALTELEIQAQWPSSFIFVQSEPQPSRAD
                     NVWQIGNLSAKTEGKIVIRGRLINKIGETNQLDVKASFRPVNLSSTFTINKEHNIILS
                     NAGVAVGLSAPDTLAIGRDNEITIAYQANSGDQTDNLQLLINNVDWADIKLIDNNGQT
                     IESLTNQSWSLAAPSAEPNNLKLIITPKSEASGLQILSLRLETAVNDRHYLIDSRDLD
                     FQLINSKLNLLLKINDSNADSGVTAGQVLNYQLSYANQGDSTLKDVRLVASLQGNWLD
                     WSSLKDKEQGQVSGQTIIWSKKELPELALLKPGTSGTINFSIKLKDWDNGNKADSGQI
                     TSYTYYQVGEDNLLSKPSDNQKSNTIINQLNSDFSFQETVVYFNEDNIAVGSGPLPLV
                     VGETTNLKVYWQLKNSLHDLRDITMVADLPDYVTWAGKDNVPAGQIRYDQDQHQVVWR
                     LDRLPANDEAMIGQFNIGITPRANQQNKIIILLPGSTATAIDNATQGNLEVTSPAKTS
                     RLEDDQIVQTDGLVK"
     gene            complement(7176..9434)
                     /locus_tag="PKV49_01510"
     CDS             complement(7176..9434)
                     /locus_tag="PKV49_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HPL22323.1"
                     /translation="MSKKSFSNIIYKQHGPKPSDFLLDLRQQIVHPVASASQRQRSRR
                     PRLKIKLNWHWPKWPRYRLTWKLRFPKLAWPKKRRRTWRFGRPQTLRPILSLVVVFLL
                     LILPFKLIVYLQDLRGMRTRIVNASNDGLTDLKSAGQLLANRDFLSASQKFNQASDSF
                     WQAQQDLRGVQSWLWQLALVVPTSQARLAASAPLITQAGDLSGQLGNDLTTVAKNITA
                     NLAAGDSILLARQLKIDSQQALDRSLVLNQVIDKINPASLPQGYRDQLVDWQDKLRLL
                     SDSLRQLNYWSGVSLDLLGDKMDRRYLLVFQNNAEARASGGFVGSYALLDISRGRIKN
                     LEIPKGGSYDTNGNLRRFVNPPSPLRLVDVYWKFWDANWWPDWPTSARQLAWFLQESN
                     GPTVDGVIAITPDIAEDLLRLTGPIDLSASHGLIITADNFRLTVQDNVESKVSGQQQP
                     KKIIGDLAAAILEKLPTVLADQHQAIELIKLLSQRINTRHILFYFNNPEIQAQWQQTG
                     WSGQLEQLEPGQDYLQVVNTNIAGGKTDLRIRQTIEQQVEIQTDGSIINDVTIIREHT
                     GEKHEGLYGVRNVNWLRLYVPLGSQLLSADGFSQPDSQFFQTIDPTAEDLPVLKNGEG
                     LAVIDSNSGTWSYQELNRTVFANWSMVDPGQTARLHFRYKLPYNLLDDNQSGWLVKIR
                     SFLGLGRFGRYRLMIDNQAGQPLSTWKVNWHWPLSWKLLWSSPPAIEPDQWQLNQLVD
                     RQTVGLLFQINN"
     gene            complement(9450..10688)
                     /locus_tag="PKV49_01515"
     CDS             complement(9450..10688)
                     /locus_tag="PKV49_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPL22324.1"
                     /translation="MMNLLFISSSELISQLINWKNSIMVQQRSGQRIGIDARLYGPSG
                     KGLGRYIQEITDRVVSLDKDNQYIIFLSPHSVGDCQINQENVEKVVMTERWYTLAEQL
                     AWPKLIRRHRLDLLHVPHFNAPLICPCRLVITIHDLILTKFPSRRASTLSPIVYWLKN
                     LAYRLVIGRAVKQAKVILTVSEFTKRDIIEQLAVPASKIVVTYNGVADFSQRRADKKL
                     ADKQVLLSYNIKQPYLLYVGNAYPHKNLEWLIRLFKIWSVNHQDFSLVLVGGHDYFYQ
                     RLAKLVASLWSGQTKAPVILPGFVPDEQLAVLYRSAALYIFPSRYEGFGLPALEAMSY
                     GCPVVSSNSSCLPEIFGQAAVYFADNQMDSAIMAIDQLLNNPSQRQQIVQRGFEQIKL
                     YSWATCAEQTLAIYRQLLKG"
     gene            complement(10624..12360)
                     /locus_tag="PKV49_01520"
     CDS             complement(10624..12360)
                     /locus_tag="PKV49_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22325.1"
                     /translation="MFRLPKINNLMIFTGAYLIIVGSLFFTATSAYQYCIQRPDFVKW
                     SSPDETANYYISKLYAEHRRLTTDNQLDWPAAEIVHPRSFKAPYGIMMPMSFLGLPLL
                     YGFLARIISVGILPYLTPFFGVLGLWLFFGLTRRLFNSSTAIVATTLAAAFPVYLYYS
                     SRSMFHNILFIVAGLGGLYCGLRALETMRQARRRLCRRWPALIWLAGSGLCIGWSLAT
                     RTSELLWLAPVILILVIVYFKEIGWYRWLWWGSWTWLAMLPAIFWNLSLNGHWYGGGY
                     PAMEQSVNTLSQTGLSLLNWHNLFNGSVLLTKLKLLKATIFHFGFWPQQSWIVFRHYV
                     VDMFPWLLPLAAIGIVFFIFSRRQWSKEKWLYLLNWLAISVFLVIYYGSWKFNDNPDP
                     NSFTIGNSYTRYWLPIYLGALPWAADGLRRLIRFLFRRQIARTVIVLSVLVICFYSIK
                     FTAVGSEEGLIYSGYRQIADYRLWSEIISKTESNSIIITRYHDKLFFPERQVVVGLFD
                     NEVINQYYAQLMATRPVYYFNFRFPPEALEYLNSRQLFKYGYILQELGQYDEFALYQL
                     VGINQSVNQLEK"
     gene            12359..14281
                     /locus_tag="PKV49_01525"
     CDS             12359..14281
                     /locus_tag="PKV49_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22326.1"
                     /translation="MKLLRNFKWPDDALLITSLVSLFGALIYWLYQLNWFGQITAWFL
                     TGITYCIIYRKRRQSKEKASTNNRINWTSWPLLIIIVGLLFYLSKQQSLNALQSPWTE
                     IGWPFWLAYWLLAAIGLWLIKSGRQISLATIAVWWLLTYSLASLIYFSAFGFDPFVHQ
                     ATEKTIIAQGQITPKTPYYLGQYALVISLHRLTKLPIDQVERFLLSGLIAIFVPWLIS
                     VWFKNRQFNQSAGRLVLWLWPMISWPIFIVTTPQNCAYFFLIITVVSATMNQINQPLL
                     WSSALASLVCQPLAGWLALTTAALASLKNFSGTKATISRWLAGSLTAIALPLSLWYAS
                     WQQTANWRLTWPPIKKLLGWLATIFNCHHLWPNQEVWWLNLIYSYQAWQPLLLILLII
                     AGWLAWRRQPLVWTRIIRPLNQSLLIGAVLTSLISFNFVIDYERYNYLQRFLWLALII
                     NLPAILLASWQWLNRWISSKDRWLNWPIIIALATAVTFGLYLTYPRVDNYSNAKGLSV
                     SAADLLAIRWIENNAPSDYIVLANQQVGAAALGEYGFYPGRHNRYLANNLFYYPIPTS
                     GQLYGYYLKMVDDYPQRSTIKEAAELAGVKQAYLVLNSYWYGFEKIAQEATMEADARH
                     SLANGQILVFSYQFSE"
     gene            complement(14302..15489)
                     /locus_tag="PKV49_01530"
     CDS             complement(14302..15489)
                     /locus_tag="PKV49_01530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22327.1"
                     /translation="MKIILANNTFYPYQRGGAEAIVRRLAEDWSAAGHQVMVVALRPI
                     KSRKDNLKYTPQGYKINYWPSRYYDLENYSCWRKLWWHLPDWLGIRYLTKWLKILRSF
                     QPDLVVANNLTGLGFSLHFCCQRLGIASAQIFHDVQYLLPSGLLVIGREQALKSWSAR
                     IYQVITAFWLAPARLLISPSQWLMTCHRQVGWLARARWQQLPNPINVRLAAEYRLPDN
                     LRRAIFVGQLSEAKGILWLADQWADFNRQLAALGFNEVDLLVAGDGPLSDHLRLLAGQ
                     DRRLILLGHLTSSQVAEQLASADVLLSPSFCYENWPTVLLESAAAGCLAISSSHGGGG
                     ELADNLGYLTFKAGDFNSLLDVWRQLAITAAKIKSWPANNLNMIDSNDYLQKIISATK
                     NLD"
     gene            complement(15486..16658)
                     /locus_tag="PKV49_01535"
     CDS             complement(15486..16658)
                     /locus_tag="PKV49_01535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPL22328.1"
                     /translation="MRIVHLVCTFPPYRGGMGSSVQQMVGELVASNQAYQVAVVCPDY
                     GQCQNNTNQQWAGYQIIRLASWLSVGNAAILKGLSQIWRSADIVHLHYPFYGTDGLVW
                     WLKKKYPKTKLIIHYHMDPQAGGWRGWFFKFFRKLFLARLLKQAEAITCASLDYWQSS
                     AAGRLKLKLADKLLAVPFRVDTERFQPRPKDQQLVDKLSLNNQRPTILMVGGLDKAHY
                     FKGVDVFLKSLASLKQVNSKLDFQAVIVGDGDLKKGYQAMAERLGLADRVRFTGAVSS
                     QQLPAMYNLADLFVLPSINRCEAFGIVLLEAMASGLPCLAANLPGVRQVIAPGVNGQL
                     IKPADWIDLAEKIASLLSDQSQLRAMAIASRQRAEQNFSLGRPSELNDIYQRLVAL"
     gene            complement(16668..17024)
                     /locus_tag="PKV49_01540"
     CDS             complement(16668..17024)
                     /locus_tag="PKV49_01540"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HPL22329.1"
                     /translation="MLQRILALAVIAFFLAKLFWQQRQRKMAWTEFCFWLIFWLASAI
                     AIIFLNQIDRLVANLGFSSSGIDVLLYSGLAVCLYFIFRLRLRLEKMDRQLTKITSSI
                     ARQQPRSKDINNTDRG"
     gene            complement(17032..17694)
                     /locus_tag="PKV49_01545"
     CDS             complement(17032..17694)
                     /locus_tag="PKV49_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL22330.1"
                     /translation="MKVICVIPAWNEAKTISQVVADVRSQVDEVVVVDDGSSDGTVDL
                     AVAAGATVLLHPINRGQGAALRTGTQYALDNGADIIIHFDADGQFVASEITEMTAPII
                     SGLAEAVFGSRFLGKEHNLPPFKKRVLLPLARLFNWFFLGVRFNDPQNGFRALSADAA
                     QRINWQQDRMAHCSEILWLTRLTGIKYQEVGVTVHYSHFGQQMSGGWRIISDLFLGRF
                     LK"
     gene            complement(17697..18722)
                     /locus_tag="PKV49_01550"
     CDS             complement(17697..18722)
                     /locus_tag="PKV49_01550"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPL22331.1"
                     /translation="MLVQKIFDKKNVLVTGGAGFIGSHLCDELIKANKVICVDNFTSG
                     NERNIDHLLSNPDFIFIRHDMTEPLDLLALPELERFKIKFQGIQEIYNLACPMSPVDF
                     MANRLANLLANSTAVFQVLEMARQYQAKLLHFSSSVVYGSREQHNGSKVSENELGIID
                     QLSDRACYDEGKRFAETMVNTYRQFHGINAKIIRLFRIYGPRLPINQGHMIPDFIINA
                     LDNKDLVINGDEKFSSSLCYVSDAVDGVIKLMQSDLPGPVNIGSEMDINITDVAKKII
                     EKTGSQSKISYNDSLLFMSKLNLPNTRLAREQLGWMPVASLDHGLDKTIEDLRSSKAI
                     KHLPTDW"
     gene            complement(18731..20845)
                     /locus_tag="PKV49_01555"
     CDS             complement(18731..20845)
                     /locus_tag="PKV49_01555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPL22332.1"
                     /translation="MQLNKYSKNFYIWGGLILSWLALIVQPAMASDSYVAADIYRAAL
                     APSDSYYSNQWYLQKIKAQIAWEERRESPDIVIAIIDSGVQINHPDLKDNIWRNSREI
                     PNNHKDDDGNGFIDDVNGWDFVNNSADPNPKFAANFTEDGVFHGTIVAGVAAAAGNNA
                     AGVAGVTWRAQIMPLKALDDQGVGSTFRIIQAIDYAINNGADIINLSFVGYGYSQSLA
                     EAIERAYRAGVIVVAAAGNEESGGYGHSLDKLPMYPACHDGDKNMVIGVASTDALDQK
                     TNFSSYGSRCVDIAAPGVSIYSTVVYAPNQQIDGQPLDKYYDGYWSGTSVSVPMVSGA
                     LALIMQQNPSLKRDELIKVLLNNADDITRLNMNYLGQLGRGRLNVASAINDASLQLVN
                     QVNRILVAPASSSSQPIRILDATGKALAEFYAFGVKFPYGLRVASGDLDNDGSPEIVV
                     VPDKGGQSAVKIFNYKGKLVGQFIAWRKDFTAGLRVAVGNIDSDKAGEIIVATGSGAK
                     NEVKVFTAQGVLKTKFRPYNQNFTGGVSLAVGDIDGDNVDEIITGAGPGGGPHVRIFS
                     GSGQLKGEFMAYESNFRGGIQVAAGNLNYGAKANQDEIIVSSGAGRQPQVRIFSAKGQ
                     LLSSFLAFNDKFRQGFSVALSDIDNNGLPDIVTSAGPGGGPHVRIFDAFGDLRQSWYV
                     YNQAYDGGVNAAALNLNKQYFN"
     gene            complement(20832..21968)
                     /locus_tag="PKV49_01560"
     CDS             complement(20832..21968)
                     /locus_tag="PKV49_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPL22333.1"
                     /translation="MVIGIDASRANRPNKTGTEWYSYHLIKQLAAIDRHNHYILYSNK
                     PLEPGLAELCQQYSNFKSKVLNWPFSRFWTLGRLTLEMIVRPPDVLFVPAHVLPLLSR
                     AKLVNTIHDVGFMAFPNLYGRHAARYLRWSTKWALAKAQRLIAISEFTKQEIQRYFSA
                     DWSKIKVVYNGYDSSFYKPESEPNHDQRILADYNIEQPYLLYIGRLENKKNMVGLLNG
                     FRQYCQDQPRSRLKLVLAGQRSADFAQVSNLLSDPILAKRCVELGWTPMTALPSLLRQ
                     AQAFVFPSWYEGFGMPILEAMASGTPVLSSNTGSLPEIGGEAVLYFDPANPVDLAAKI
                     EVVIKNNDLRQRLIKAGLKRAAGFSWTKTAQATLKILQEAHAAQ"
     gene            complement(21973..22980)
                     /locus_tag="PKV49_01565"
     CDS             complement(21973..22980)
                     /locus_tag="PKV49_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPL22334.1"
                     /translation="MFINRIAIDLGTTYTLVHLPKKGVIVNEPSVVAVSMTDKKILAV
                     GDEAKDMVGRTPDTIIAIKPLKDGVIADYHTTEVMIRYFINKALGGIRLFRPEVMVAV
                     PAGITSTERRAVIDATLGAGAKAAYLIKEPVAAAIGAGIPVGSASGHMIIDIGGGTAE
                     MAVISLGGVVTATSVRVGGNKIDLAIIEYIRRKYNLAIGERTAEEIKIGVGSALYLTD
                     KLSINIKGRDILTGLPKTVTVTSDDVTEAIQHELEAIIGAAKNILRETPPELASDIME
                     KGIVLSGGTALLCNLDQLIARTTDVPVFIADDPRTCVVKGTGIALENLDSYKRSIFAT
                     R"
     gene            complement(23062..24006)
                     /gene="murB"
                     /locus_tag="PKV49_01570"
     CDS             complement(23062..24006)
                     /gene="murB"
                     /locus_tag="PKV49_01570"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPL22335.1"
                     /translation="MKQRPVFIQENVRLAPLTTYQIGGPAQFLAKINSLEELTSAIDW
                     ATEDHWPIYFLGGGSNLLISDQGVAGLVITLTEPEPIFTDKIVEVGAGHSLSKLVTAC
                     IDHGLAGLEWAAGIPGQVGGAVRGNAGAFGGWLADRVIEVVAYNIKSKQIEVISAQDC
                     QYAYRHSIFKDRAELLIWSVKLALEIGNQAELQKIADEHRSYRRQRHPLLPSAGCVFK
                     NPAVDEVARLAPKLLQAAQSVGAVSRGEVSAGFIISQAGLAGYSIGPAQISRQHSNFL
                     VNNGGATAEQMRQLIEYVKTEIRHQYNLEFIPEVALWP"
     gene            complement(24016..25464)
                     /gene="murC"
                     /locus_tag="PKV49_01575"
     CDS             complement(24016..25464)
                     /gene="murC"
                     /locus_tag="PKV49_01575"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPL22336.1"
                     /translation="MDWLAIKHIYCIGIKGVGMTMLAQFLQSRGYQITGSDVAESFMT
                     DQILADHNIIVRTGFQFNEQLNSADLVIYSTAYNQDNPEVAWALDNRPTLTYAEALGD
                     VFNQYYGIAVAGSHGKTTVSAWLGFTLWQGGLKPNVLVGSRVPQFNGASLLGSSSLMV
                     IEADEYQNKLRYFQPKMVVLNNIDYDHADFYPTPADYQQAFIDFLAKIPTDGYLIANY
                     DDPLIRRLAPVHCRGRIISYALDNAADYLAYDLKAVNGRQYFKVKMGIDLDDIAEEDE
                     KLSKLAVSNAGNLGDFSTSLPGRHNISNALAVLAASVELGLQLYKIREGIAEFIGTAR
                     RLEVLGQFQGATIIDDYAHHPTEIKASLAALRQQYPDFKLRVFFHPHTFSRTLALLDG
                     FVNSFNEADEVGILKIYGSAREEAGGISGQELAQRITVNRAKAGLKDEVSYFEDMARL
                     DAYLRQSAKSGEVIVLMGAGDIFRIGQQLLVK"
     gene            complement(25466..26545)
                     /locus_tag="PKV49_01580"
     CDS             complement(25466..26545)
                     /locus_tag="PKV49_01580"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPL22337.1"
                     /translation="MIKTRSNKKIILLTGGGTAGSVIPLLAIAKVLRDYKSDCHFIWL
                     GTNRGPERQLVEEAGIKFQPISAGKLRRYWSWQNILDIGRLIIGFLQANYFLNRIRPD
                     LILSAGSFVSVPIAWAAKFYGIPVIIEQLDYRPGLANKLMAPLAKKILVTFAKSGRDY
                     GRKAVWLGAPIRPDILQTVGDNPSLLWPFNGQRSIILIVGGGTGAAGINNLVVQQLSG
                     LTELADIIHLTGRGKLGAKPQEHYQPLDFLNQAAMMAAYQAADIVVARAGLGTLIELA
                     ALKKVSLIIPMPASHQLDNAQAIKEAGAAIVVPEKDLVDGQLVRQLKQLLADKQKQVD
                     LINNWPKAIKIASQSEIIQVFQEYL"
     gene            complement(26542..>27018)
                     /locus_tag="PKV49_01585"
     CDS             complement(26542..>27018)
                     /locus_tag="PKV49_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPL22338.1"
                     /translation="VLKPGYSPDKSCYQVNQSLIAIGSGGFWGRGLGESRQKYLYLPE
                     VQNDFIFPIIAEELGWWFSVFLVLLYFALFYRGFQLAKAAPDLYGQILAGGIVSGIMI
                     QVFINLGGTINLFPMTGVPLPLISYGGSSLMSLLFGLGILANISRQVRLPDNLPKL"
BASE COUNT         8443 a         6245 c         5105 g         7225 t
ORIGIN      
        1 aatgagcgcc acgccgaaaa ttaaacccga tccccgcgat ttggcgcgcg gaacagctgc
       61 ttatgtattt gtagagccaa aagatgtgat tgacgagggg atgattaaaa aagagctcat
      121 tatcaacgag aaaattgacg agattataga cgacgagatg gattcacaga aggttgtttt
      181 agaaactgct tataaaaaac gattggaact gaaaaaattt tttgaagccg aaaagtcaaa
      241 cattaatcca cttgttttgg tgcagatacc ggttgccgaa gcaggcgaag ataaaataag
      301 agctgtcaaa aagtttttag cggataagga cataaccgaa cgaaaaaatg gacaagggaa
      361 tggcaagctt gctatttggt tatccgagca aaaatccgaa acgcttgatt ggatagcaga
      421 gccggacaat gaaattgaat ttttgatttt caaacaagcg atcgatactg gttgggattg
      481 tccgcgcgcg catatccttg tgaaattccg agaatcacac agcgaaactt ttgaaatcca
      541 aactgtcggg cgtattttgc gtatgccaga acaaaagcat tatgcaagcg aagatttaaa
      601 tcgtggatat atttacacta atgttcaaag cattatcgtc aaaaaagaag aatataatcc
      661 aaatatcatc aaacacctaa aggcaacgag aaaagatgtt tataggccga taaaactgac
      721 ctcttattac aaatcacgcg cggattacgg cgatattact tctagtttta cgccggtgtt
      781 tgaaaaaact gcttgtgagg agtttggctt gaaaggtgat catacttttt ttgctcaaaa
      841 tgttaaaaaa gttgaagaca aaggtgtggt tttggatatt aaaaaatatc aacaagagat
      901 aatcgccaat gccagaataa aaggtaaaac ttttgatgaa attgaaggca aaattaactc
      961 agcagatcgt gcccgcttga taattgcggg aaatgacctg caagcgttat ttgagcaaat
     1021 tatcaaaaat aatcttggtt cgttcaaaaa tatcaagcgt tccgttccga ccgttaagac
     1081 cgctatttat tcgtggttta aaaaatatct aggttcaaaa gaatggtcgg aggaaataat
     1141 tttggtgcaa atgatttttg tccacaatgg taatcgcaag aagtttgaga aaattttagc
     1201 tagcgcgatt gagaaataca aagcagtgcg cgaaaaagag attttgaagc gtgttgaaga
     1261 aagcgagcag ttttacgatt ttgaaattaa aaaagagagt tctttcaatc aatatattga
     1321 tgagttgatg agttataaaa aatatgtcta tgatccatgt tatttaagtg taaatcgttc
     1381 aataccagaa aaaaattttg aaaagttttt ggttgaaaac gatgataaaa tagtttggtg
     1441 gtggaaaaac ggcgaaaaca agcaagatta ttttggcatt aaatacgaat atcccgtcgg
     1501 agtaattcac actttctatc ctgattatct cgcgcaattt actgacgggc gaatagggat
     1561 ttttgagaca aaagatacgg gtgatcgcga cggcgggaca tataccaagg cgaaggcgga
     1621 aaaattgcag gaatatatca aaaatcagaa aagccaaaaa cttttcggtg gtatcgtgat
     1681 tgagaaaagc gatggctgga aaattaacca aaaaccattg tataattgga gcaaatgcga
     1741 acgaaacgat tggagtgatt gggacaaatt gaaaatttaa ggcggcgtgc taataatatt
     1801 agtacatcct taatccgtca aaaaagaatt gtgaaagtga tggcgaggcg gaaagggagt
     1861 atggggggat tctgccacgc ccgagcgaat acaaaaaacg gacagccccg ccactctgct
     1921 cgttcagagc agaccgacaa ataaaatttt gttttccttg taggaaaacg ggcgtacgcg
     1981 aataaaaaat tgtaaacaaa actttctttg gcggagcgaa tcattgttcg ttttgttcaa
     2041 aaaaagttcg aattttaacc aaaaaacacc gctaagtttc acttttcgta aaatcaaaaa
     2101 acagagatga aaagattact actaattatt attctcatta ttgtaataat tggagttttt
     2161 ctgctttggt actcaccagt tatttttcaa gaaggtcatc cttggccaca acttaaagga
     2221 atcattcagc taaactttac caataaagat ctagtcaaat tatccagtga aaaaaataaa
     2281 tggcttacga aaagtaaaaa tggtgctgcg gttatcaaat catacatgaa aaatcagggt
     2341 tatgatttta ctgaacaaat gggatcagca tattttttcc aatcagctac aggcgcacaa
     2401 attattgcaa cacatagata ttacagccga ttttattcac tatggacaat gatagaaacc
     2461 atcaataaca cagacgacgc ggaacaatta tctattgctg aggaattggc agattgtctg
     2521 cctaaaagtg acacagccag ccgtgaccaa tgcactactc ttttagccac aattaaaaac
     2581 tttaccgatt gtgctaatgc tggttttaca attatccaat ccgatccacc tcaatgctcc
     2641 acccctgatg gtcgatcctt tactgacaac actattagca gttgggaagt ggctgtagct
     2701 agtctaaata attgtgaagt taaaagcatt tttcaggctc atagcaaact agtagctcta
     2761 aaactcaaaa atggtaatac catactcacc tacgaacccc aaattgatga tgtgatgaaa
     2821 atcgctgttg acttaacatc taaatgtggt gctattcaca tggcgactga gtaattaatg
     2881 gagattagag atacggttga tggcaaaatt tacggaaaga gttataatga tattataagc
     2941 cagactgccc gcttgggctg ccaaccaatt attaataata acgcctccat gacgactcgc
     3001 tatgaataaa attgactggc caaaactaat cggttctcta gttataaccc ttggagctgg
     3061 ctggcttggc tctagcctga ccagaccaaa taacagttgg tatgccaata ttaacagaac
     3121 cgtcttgacg ccacctaatt ggctttttgc gccagtttgg acaatcttgt tttgtttaat
     3181 ggctgccgcc ctgtatatta tttggtcagg ccgcaacaaa aagaagacta gaaaaaaggc
     3241 ggtccaatta tattttggcc aattagccgc caatatcgcc tggtcaggta tttttttcgg
     3301 cctacagagt attagtctag cttttattga aatagttttt ctactgattt taatcaccta
     3361 ttgtctagtg gcttttagtc gcatcaatcg ggcggccggc tatctgatgc tcccctattt
     3421 tttatgggtt tgtttcgcta ctgttcttaa cttatctttg tggttaatta attagtggct
     3481 gccgagataa ataaaaaata ccctagtcgg gtatttttta tttattatat tattataata
     3541 attatcttaa tagtcgacta acatcttgaa aagttattaa aataatcagg cttaataaca
     3601 gaataaaacc aatattgtga accaaggctt caatctgcgc cttggtccga tgaccggtaa
     3661 tcttttctat ggcaataaac aacaagcgcc cgccatcgag agccggcaga ggtaaaatat
     3721 taataaaagc cagattaaga gatagcaacg ccacaaattg taaaagataa ataaagccaa
     3781 gactgctaat ctggcctgtt aaaacagcaa tgcccactgg accagccacc tgatcggcca
     3841 tattgtggcc atgaaatagt ccgtttaata attgccaaaa accaacagca atatctttta
     3901 gactcagcgc tgtcagtcgc cacccttccc agatagctcg tggccaagca taactcacta
     3961 aaccgctttc agccagagcc gcgccgatag tgtattgaga attattgcca tccatcattt
     4021 gcggcttaac ctgaatatct aactccgtct cttgccgctt aatctggtag ttaagcggct
     4081 ggccggctga ctcttggttc aattgacgca attgattgat gctagcgatg gtctgttgat
     4141 taatgctagt aattagatca ccagcttgca gaccagcttg attggctggc gaatcgggta
     4201 taacttcaat tatcgctatc tgctgattag ctactcgcgc ttgagggcct aaattattgg
     4261 tcgcttgtgg ccaaccaacg ctaagaccaa tgataaaaat tatggcggct aaaactatat
     4321 tcattaccac tccggcaatc accactaaaa ttcgttgtcc ggctttttta ttactaaagc
     4381 tctgcggatc attaggccga tcaataccat cttcaccgtg cagcttcaca aaccctccca
     4441 agggtatcca gttgagagaa aaaatggtgt ggcgatggcg tggcggccgc caacctatag
     4501 ttcgccaatg gccgcggcca tttttaaacc aaccgattaa tcgcggcgga aaacccaacc
     4561 caaattcctc aactccgaca ccattccaac gtgccgctaa aaaatggccc aattcatgaa
     4621 caaccactaa aacacttagt acggctaaaa aaataaccac tgtcatatta gatagtcata
     4681 atttctacct ccttcttgtc gcgtagagct tttaattctt catttttttt acccaaaaaa
     4741 tcatctaatt ctttaatgaa acgaaactta ttatcttcgt tgatttcctt agcgctaaaa
     4801 gccgcctcaa tcttattttt aacctcttca cgcacctggc gcaagacaat ccgcgattct
     4861 tccaatttct catttagacg cctgactaat tcgcgacgat tttcttcatt gagcgctggc
     4921 atagttaagc gcagcttatc cccctcgtta attattccca tacctaaatc ggccgcggtt
     4981 aaactcttct caatattttt caagacagat cggtcccatg gagtcaccac taaacatcgc
     5041 gcgtcttgaa cgctgatatt ggccatggca ttaattggtg aaaagctgcc ataagcctca
     5101 atggaaatat tgtccaacag gccgggatgg gctcgtccgg tgcgcaggct ggcgatatct
     5161 ttaataaaaa aatcaattgc tttttgaaaa tcagctgact gattgtcaat aaattggttc
     5221 ataaaatact ttaacaaatt atttaagact tatttaacta atccatcagt ctggacaatc
     5281 tgatcatctt ctaggcgact ggtcttagcc ggactggtga cttccaaatt accctgagtg
     5341 gcgttgtcaa tcgccgtcgc tgttgagccg ggtaacaaaa tgataatttt attctgttga
     5401 ttagcccgcg gcgtgatacc gatattaaat tgtccaatca ttgcttcatc attagccggc
     5461 aaacgatcta gccgccaaac aacttgatgc tgatcttgat catagcgtat ctgaccggcg
     5521 ggaacattgt ctttaccggc ccaggttaca taatccggca aatcggcaac catagtaata
     5581 tcacgtaaat catgaagact attcttgagc tgccaataaa cttttaaatt agttgtttca
     5641 ccgaccacta acggcaaggg ccctgacccg acagcaatat tgtcttcatt gaaatacacc
     5701 actgtttctt gaaaactaaa atcactattt aactgattga taatggtatt acttttttga
     5761 ttatcgctcg gctttgataa taaattgtct tcaccgacct gataataagt ataactagta
     5821 atctggccgc tatcggcttt attaccatta tcccaatctt taagtttaat agaaaaatta
     5881 atagttccgc tggtgcctgg ctttaatagc gcgagttccg gtaattcttt tttactccaa
     5941 ataatcgttt gcccgctaac ttgtccttgt tccttgtctt tcaatgacga ccaatctaac
     6001 caattgcctt gtaaagaagc gactaagcgg acatctttaa gcgtgctatc gccttgattg
     6061 gcataactta actgatagtt gagcacctgt ccagcggtta ctccactatc agcgttgcta
     6121 tcattaatct ttaataataa attcaatttg ctattaatta attgaaaatc aagatcacgg
     6181 ctatcaatta aataatgacg atcattaacg gccgtctcca gtcttaatga taaaatctgc
     6241 aaaccgctag cttcgctctt gggggtaata attaatttta gattattcgg ctcggcacta
     6301 ggcgccgcta aggaccaaga ttgattggtc agcgactcaa tagtctgacc attattatca
     6361 attaacttaa tatcagccca atcaacattg ttgattagca attgcaaatt atcagtttga
     6421 tcaccggaat tagcctgata agcgatggtg atctcattgt cacgaccaat ggccaatgtg
     6481 tctggcgctg ataaaccgac agctactccg gcattactaa gaataatatt atgttcttta
     6541 tttatagtaa aagtactact aagattgacc ggccgaaaac tggccttgac atctaattga
     6601 ttggtctcgc cgattttatt aattaaccgg ccgcgaataa ctatcttccc ctcagtttta
     6661 gctgacaaat taccaatctg ccaaacatta tcggctcggc tcggttgcgg ttcggattga
     6721 acaaaaataa agctgcttgg ccattgcgct tgaatttcca attcggttag ggccaccgat
     6781 ccattattac gataattgat agtataggta aactcttggc cagcggttaa gttcttgtcg
     6841 gtctcaatgt tgatagtcag cagatcagcc ttggctcgat aaccggtgta atagttatag
     6901 cccagccaac cagccaatac taaaattagg ctatataaca taaacaacaa aactttaacc
     6961 caccaacggc gacgacgaac agtaaaacga ctgacatcaa ctatctgtcc ttgatcattt
     7021 tgataaattt ctaatagact atcatcaacc tcctgctctt tagcttcagc tcggtcaata
     7081 gaacggctag ctttcttacg gattggctga ccgactttaa tcatttgttc aaagtaatct
     7141 atcgccggtt tttttggtgt tcttgtcata gacgcttaat tattaatttg aaacaataaa
     7201 ccaaccgtct gccgatcaac cagctgattc aattgccatt gatcgggttc aatagctggc
     7261 ggcgatgacc atagtaactt ccaagacaat ggccagtgcc aattaacttt ccaagttgac
     7321 agaggctgtc cagcctgatt gtcaatcatt aaccgatagc gaccaaaacg gcctaatcct
     7381 aaaaatgatc tgattttaac cagccaaccg gactgattgt cgtctaataa attatagggc
     7441 aacttataac gaaaatgcag tctagctgtt tggccgggat caaccattga ccagttagca
     7501 aagactgtcc gattcaattc ctgataactc caagtgccgc tgtttgaatc aataacggct
     7561 agtccttcgc cgtttttaag aactggcaaa tcttcagccg tgggatcaat ggtctgaaaa
     7621 aactgactat ccggctgact aaaaccatcg gcgctcaata actgactgcc taatggtaca
     7681 tagaggcgca gccaattaac attacgaacg ccatagagac cttcatgctt ctcgccagta
     7741 tgttcacgga taatggttac atcattaatg atgctgccat cagtctgaat ttccacctgc
     7801 tgttcaatcg tctgtctaat acgcagatca gtcttgccgc cggcaatatt ggtattgacg
     7861 acctgcaaat aatcttgccc gggttccagc tgttcaagct gtcccgacca gccggtttgc
     7921 tgccattggg cttgtatctc cgggttgttg aaataaaaca gaatatgccg agtgttaatc
     7981 cgttgagata atagttttat caattcaatc gcttggtgtt gatcagccaa gacagtcggt
     8041 aatttttcca aaatagcagc cgctaaatca ccaattattt ttttcggctg ctgctgtccg
     8101 ctgactttac tctcaacatt gtcttgaaca gttaaacgaa aattgtcggc cgtaataatt
     8161 agcccgtggc tggccgataa atcaatcggc ccagtaagcc gtaataaatc ttcagcaata
     8221 tctggggtta tagcaataac tccgtcaact gtcggcccgt tgctttcctg taaaaaccaa
     8281 gccaactgac gagctgatgt cggccaatcc ggccaccaat tagcatccca aaatttccaa
     8341 taaacatcga cgagtcttaa tggactgggc ggattgacaa aacgccgcag attgccattg
     8401 gtatcataac tacccccctt gggtatttct agatttttaa tccgacctcg gctgatatcc
     8461 aataaagcat agctgccgac aaaaccgcca ctagccctag cttcggcatt attttgaaaa
     8521 actagcaaat aacgacgatc catcttgtcc cctaataaat ccagactaac tcctgaccaa
     8581 taattcaatt ggcgaagcga atcgctgagc aatctgagtt tatcttgcca atccactaac
     8641 tgatcacgat agccttgagg cagtgaagct ggattaattt tatcaataac ctgattcaaa
     8701 accaggctgc gatctaaggc ctgctggcta tcaattttta actgacgggc taataatata
     8761 gaatcaccag cagctagatt ggcagtaata tttttggcta ctgtggttaa atcattgcct
     8821 aattgtccag ataaatcacc ggcctgagta attaatgggg cgctggccgc taaacgcgcc
     8881 tgactggtcg gtaccactaa agctaattgc cacaaccatg attgaacgcc gcgcaaatct
     8941 tgctgagctt gccaaaaact gtctgacgct tgattgaact tttgactagc tgataaaaaa
     9001 tcacgattag ctaacaattg tccagccgac tttaaatcgg tcaacccatc gttggaggca
     9061 ttgacgattc gtgttctcat tccgcgcaaa tcttgaagat aaacaatcag tttgaatggc
     9121 aaaattaaca ataagaaaac aacaactaaa cttaatatcg gccgtaatgt ctgcggccga
     9181 ccaaaacgcc aagtccggcg gcgttttttg ggccaagcca atttaggaaa tcttaatttc
     9241 caagtcaaac ggtaacgcgg ccattttggc caatgccaat taagctttat ttttaatcgc
     9301 ggccgccgcg accgttgacg ctggctagcc gaagcgactg gatgaactat ctgttgacgc
     9361 aaatccaata aaaaatctga gggcttcggc ccatgttgct tataaataat attggaaaaa
     9421 gattttttac tcatgatggc tatttatttt taacccttta ataattgacg ataaatcgcc
     9481 aatgtctgct cagcgcaagt cgcccagcta tataatttaa tctgttcaaa tcctcgctgg
     9541 acaatctgct gacgctggct aggattattt aataactggt caatggccat tatagctgaa
     9601 tccatttgat tatcggcaaa ataaactgcc gcttggccaa aaatttccgg taagcaagaa
     9661 ctgttgctag aaacgactgg acaaccataa ctcattgctt caagtgctgg caaaccaaat
     9721 ccttcatagc gtgacggaaa aatatataac gccgccgaac gataaagaac agctaattgc
     9781 tcatctggca caaagcccgg caaaataacc ggcgccttgg tctggccact ccacagacta
     9841 gcaactagct tagctaaacg ttgataaaaa taatcatgac cgccgactaa gactaagcta
     9901 aaatcctgat gattaaccga ccaaatttta aataaccgaa tcagccactc taaattcttg
     9961 tgcggataag cattaccgac ataaagcaaa tacggctgct tgatattata gcttaaaagc
    10021 acttgtttgt cagctaattt tttatccgct cgccgttgag aaaaatcagc gacaccatta
    10081 taagtaacga cgatttttga tgccggaacc gccagctgtt caataatgtc acgcttagta
    10141 aattccgata ccgtcagaat aaccttggcc tgcttaaccg cccggccaat aaccaagcgg
    10201 taggctaaat ttttcaacca atatactatt ggcgacaatg tgctagcccg ccgactggga
    10261 aatttggtta agattaagtc atggatagtg ataactaagc ggcaaggaca gattaacggc
    10321 gcattaaaat gcggcacatg gagtaagtcc agccgatggc gacgaatcaa cttcggccaa
    10381 gctaattgtt cggctaaagt ataccaacgc tcagtcataa caaccttttc aacgttttct
    10441 tgatttatct ggcaatcacc gacactgtgc gggcttaaaa aaataatata ttgattgtct
    10501 ttatcaagac taactacccg atcggtgatt tcttgaatat aacgccccag ccccttgccg
    10561 gatggaccgt agagccgagc atcaatacca atgcgctgac cggatcgttg ttgaaccata
    10621 atactatttt tccaattgat taactgactg attaattccg acgagctgat aaagagcaaa
    10681 ttcatcatat tgtcccagct cttggagaat ataaccatac ttaaacaatt ggcgactatt
    10741 taaatactcc agcgcttccg gcggaaaacg aaaattaaaa taataaaccg gccgagtcgc
    10801 cattaattga gcatagtact gattaatcac ttcgttatca aataaaccga cgacgacttg
    10861 gcgctctgga aaaaagagct tgtcatgata acgagttata ataatactat tgctttcagt
    10921 cttggatatt atttccgacc ataggcggta atcagcaatc tgccgatagc cgctataaat
    10981 taaaccctct tctgaaccaa ccgctgtaaa tttaattgaa taaaaacaga tcaccaacac
    11041 agataacaca attacggtcc tagcaatttg acggcggaat aaaaagcgga ttaaacgacg
    11101 caaaccatca gctgcccaag gcaaagcccc caaataaatc ggcagccaat aacgggtata
    11161 ggaattaccg atagtaaaac tattggggtc aggattatca ttaaatttcc agctgccgta
    11221 atagataacc aaaaaaactg aaatagctaa ccaatttaaa agatacagcc atttttcctt
    11281 ggaccattgg cggcggctaa aaataaaaaa cacaattcca atggcggcca acggcaataa
    11341 ccatggaaac atatcaacaa cataatgacg aaaaacaatc catgattgtt gtggccaaaa
    11401 gccaaaatga aaaatcgttg cctttaatag ctttagtttg gttagtaaaa ctgaaccatt
    11461 aaataaatta tgccaattta atagcgataa accggtttgg ctaagggtat taactgactg
    11521 ttccattgcc ggataaccgc caccatacca atgcccattt aaagacaaat tccaaaatat
    11581 tgccggcaac attgccaacc aagtccaact accccaccac agccaacgat accagccgat
    11641 ttctttgaaa taaacaataa ccaatattaa tataaccgga gccagccata ataactccga
    11701 tgttctagtg gccaaagacc agccaataca taatccagaa ccggccaacc agattagagc
    11761 cggccagcgg cggcataatc ggcgacgggc ctgacgcatt gtttctaagg cccgcaaacc
    11821 gcaatacaag ccgcccaagc cggcaacaat aaacagaata ttgtgaaaca tgctcctaga
    11881 gctgtaatac agataaactg gaaaagccgc tgccaatgtg gtggccacaa tagcagttga
    11941 agaattaaat aatcgtctag ttaagccaaa aaatagccac aatcctaata ctccaaaaaa
    12001 cggtgtcaaa tacggtaaaa taccaactga aataattctc gccaaaaatc catataataa
    12061 tggcaaaccc aaaaaactca ttggcatcat aattccatag ggcgccttga aactcctggg
    12121 gtgaacaatc tccgccgccg gccaatccag ctgattatca gtagttagcc gccgatgctc
    12181 agcataaagt ttgctgatat aataattggc cgtctcgtcc ggcgaagacc atttgacaaa
    12241 atcggggcgc tggatacaat attgataagc gctagtggca gtaaaaaaaa gactaccaac
    12301 aataatcaga taggctccag taaaaatcat taaattattg atctttggta aacggaacat
    12361 gaaattatta agaaatttta aatggcccga cgacgctttg ctgattacca gtctagtcag
    12421 tctattcgga gcgctaattt attggctata tcaattaaat tggttcggcc aaataaccgc
    12481 ctggttttta accggaataa cctattgtat tatataccga aaacgacggc aaagcaaaga
    12541 aaaagcctcg actaataatc gaataaattg gaccagctgg ccactgctaa ttattatagt
    12601 cggcctctta ttttatttat ccaaacagca gagcctaaat gccctgcaat cgccttggac
    12661 cgaaattggt tggccttttt ggctggctta ttggttgctg gctgctatcg gcttatggct
    12721 aattaagtcc ggccgacaaa tctctctagc caccattgcc gtttggtggc tactaactta
    12781 tagcctagct agcctgattt atttttcagc ctttggtttt gatccgtttg tccatcaagc
    12841 cactgaaaaa acaattatcg ctcaaggcca aatcacgcca aaaaccccat attatcttgg
    12901 gcaatatgct ctagttatta gtcttcatcg tttaaccaaa ttaccgattg accaagtgga
    12961 acgattctta ttgagcggat taatcgccat ctttgtcccc tggttaatta gcgtttggtt
    13021 taaaaatcgc caatttaacc agtcggctgg acgattagtg ctttggcttt ggccaatgat
    13081 tagttggcca atcttcattg ttaccacacc gcagaattgc gcttactttt ttttaatcat
    13141 taccgttgta agcgccacga tgaatcaaat taatcagccg ctactctggt catcggcctt
    13201 ggctagttta gtttgccagc cgttagctgg ctggctggcc ttaacaacag ccgctctagc
    13261 tagtttaaaa aacttttccg gcaccaaggc aactatcagc cgctggctag caggcagctt
    13321 aacagctatc gctctaccgc tatcattatg gtatgccagc tggcagcaaa ccgctaattg
    13381 gcggctaact tggccaccga taaaaaaact gttaggttgg ttagccacaa tttttaattg
    13441 ccaccatctc tggcccaatc aagaagtctg gtggctgaat ctgatttata gctatcaagc
    13501 ttggcaaccg ctactcttaa ttctgctaat tatagctggc tggctggctt ggcgtcggca
    13561 accacttgtt tggacaagaa ttattcgacc actcaaccaa tccttattga ttggtgcagt
    13621 tttaactagt ctaatttctt ttaattttgt cattgattat gagcgctata actatctgca
    13681 acgtttttta tggctggccc taattattaa tctgccggcc attctactgg ctagctggca
    13741 atggcttaac cgctggatta gttctaaaga tcgctggtta aattggccga ttataatcgc
    13801 tttggcgact gctgtgactt ttggtctcta tctgacttat ccgcgagtgg acaattattc
    13861 taatgcgaaa ggattatcgg ttagcgctgc cgatttattg gctatccgct ggatagaaaa
    13921 taatgcccca agtgattata ttgttttagc caatcaacaa gtcggagctg ccgcattggg
    13981 tgaatacggc ttttaccccg gtcgccataa tcgctatcta gctaataatt tattctatta
    14041 cccgatacca acatctggcc aattatatgg ctattatcta aaaatggttg atgactaccc
    14101 tcaacgttct actattaaag aggccgccga attagctggc gttaaacaag cctatttggt
    14161 tttaaatagt tattggtatg gctttgaaaa aatagctcaa gaagcgacta tggaggctga
    14221 tgctaggcac agtttagcta atggacaaat acttgttttt agctatcaat tttctgaata
    14281 attaccaaaa acatttagat attaatctaa attttttgta gctgatatta ttttctgcag
    14341 ataatcattg ctatcaatca tattcaaatt gttagccggc caagatttta ttttagctgc
    14401 cgtaattgct agctgccgcc aaacatccaa taggctatta aaatcaccgg ccttaaaggt
    14461 taaataacct aagttatcag ccagttcgcc accaccgcca tggctactgg aaatagccag
    14521 acagccggcc gcggctgatt ccaataaaac cgtcggccaa ttttcataac aaaaagatgg
    14581 cgacaataaa acatcggccg aggctaattg ttccgctact tggctgctag tcaaatgacc
    14641 taataaaatt aatcgccgat cctgaccagc taatagtcgt aaatgatcgc taagcggacc
    14701 gtcaccagcc actaataggt caacctcatt aaatccaagc gctgctagct gacgattaaa
    14761 atcagcccat tgatcagcta accacaatat tcctttcgcc tctgatagtt gaccaacaaa
    14821 aatcgctcgt cttaaattat ccggtaaacg atattccgcc gctagacgaa catttatcgg
    14881 attaggcaac tgctgccaac gagcccgagc taaccagccg acttgacgat ggcaagtcat
    14941 taaccactga gacggactaa ttaatagtcg ggctggggcc aaccaaaaag cagtaataac
    15001 ctgatagatt ctagctgacc atgatttgag agcttgttct cgcccaatta ccagcaagcc
    15061 gcttggtaat aaatattgaa catcatggaa aatctgcgcg ctggcaatcc ctagccgctg
    15121 gcaacaaaaa tgcaaactga aaccaagtcc agttaaatta ttagccacca ctaaatcagg
    15181 ttgaaagctt cgcaaaattt ttagccattt agtaagatag cggatcccca accaatccgg
    15241 tagatgccac cacagtttac gccaacaaga atagttctct aaatcatagt aacggctcgg
    15301 ccaataattt attttatacc cctggggtgt atacttaaga ttatccttcc ggctctttat
    15361 tggccgcaaa gctacaacca taacctgatg accggcagcc gaccaatctt cagccaagcg
    15421 tcggacaatc gcctcggcgc cgccacgctg ataaggataa aaagtattgt tggctaaaat
    15481 aattttcata aagcaaccag tcgttgataa atatcattga gttcactggg gcggcctaga
    15541 gagaaattct gttcagctcg ttgacgactg gcaatcgcca tagcgcgaag ctgtgattgg
    15601 tcactgagaa gactagcaat cttctcagcc aaatcaatcc aatccgccgg cttaatcaat
    15661 tgaccgttaa caccgggagc aattacctgt ctgactcccg gtaaattggc agccaaacaa
    15721 ggcaagccgc tagccatcgc ctctaagaga acaataccaa aagcctcaca acgattaatt
    15781 gacggtaaga caaataaatc agcaagatta tacatagccg gcaattgctg tgagctaacc
    15841 gcgccggtaa aacggacccg atcagccaac cccaatcttt cagccatagc ctgatagcct
    15901 tttttcaagt cgccgtcacc gacgataacg gcttgaaaat ctaatttgga attgacttgt
    15961 tttaaactgg ctaaagactt tagaaaaaca tcgactcctt taaaataatg ggctttatct
    16021 aaaccgccaa ccattaagat agttgggcgc tgattattca aactaagttt atccactaat
    16081 tgttgatctt tgggccgcgg ctgaaaacgc tcagtatcta cccgaaaagg taccgccaac
    16141 agtttgtcag ccaattttaa cttcaaccga ccagccgccg atgattgcca ataatctaac
    16201 gaggcgcaag taatcgcttc agcctgtttt aatagccgag ctaaaaacaa ttttcgaaaa
    16261 aatttaaaaa accagccgcg ccatccgcca gcttgcggat ccatgtgata atgaattatt
    16321 agtttggttt taggatattt cttttttaac caccaaacaa ggccatcggt gccataaaac
    16381 ggataatgca aatgaacaat gtcggctgag cgccaaatct ggctcaaacc cttcaagatt
    16441 gcagcattac caacactcaa ccagctggcc aatcgaataa tctgatagcc agcccattgt
    16501 tgattagtat tattttggca ttggccataa tcaggacaaa cgactgctac ctgatacgcc
    16561 tgattgctag ccaccaattc accgaccatc tgttggacgc tgctacccat accgcctcga
    16621 taaggcggaa aagtgcaaac cagatggacg attctcataa ttgttaatta ccccctatcg
    16681 gtattattaa tatctttaga tcgcggctgc tggcgagcga ttgaactagt aattttggtt
    16741 aactggcgat ccattttttc aagacgcaaa cgtaagcgaa aaataaaata taagcaaaca
    16801 gccaagccgc tatataataa aacatcaatg ccgctgctgg aaaatcctag gttggccacc
    16861 aagcgatcaa tttgattaag aaaaataatg gcaatcgctg atgctagcca aaaaataagc
    16921 caaaagcaga attctgtcca agccattttg cgctggcgct gctgccagaa caatttagct
    16981 aagaagaaag caatcaccgc caaggctaat attctttgca acatataatt actatttaag
    17041 aaaacgtcct aaaaataaat cgctaataat tcgccaacca cctgacatct gctgaccaaa
    17101 atgactataa tgaacagtta ccccaacctc ctgatacttg ataccggtta gacgggttaa
    17161 ccacaaaatc tctgaacagt gagccattct atcttgctgc caattaattc gttgggcagc
    17221 gtcagccgaa agggctcgaa aaccattttg tggatcatta aatctaacgc caagaaaaaa
    17281 ccaattaaac aaccgagcta gcggtaataa aactcttttc ttgaacggcg gcaaattgtg
    17341 ctctttaccc aaaaatcgcg aaccaaaaac ggcctcagcc aaaccgctaa taatgggagc
    17401 agtcatttcg gttatttcgg aagcaacaaa ttggccgtca gcatcaaaat gaataataat
    17461 atcggctcca ttgtctaaag catattgtgt tccagttctt aatgccgccc cctggccgcg
    17521 attaattgga tgcaacaata ctgtagctcc agcggccacc gctaaatcaa ctgtcccgtc
    17581 gctggagcca tcatcgacaa ccacaacctc atccacttga gatcggacat cggccaccac
    17641 ttggctaata gtcttggctt cattccaggc cggtataacg caaattacct tcataattac
    17701 caatcagttg gtaaatgctt aattgccttg ctcgaacgta aatcttcaat cgttttatct
    17761 aatccgtgat ccaaactggc caccggcatc cagccgagct gttcacgagc caagcgagtg
    17821 tttggcaaat tgagtttgct catgaataac aaactgtcgt tataactaat tttggattgg
    17881 ctgccagttt tttcaataat cttcttggcc acatcagtga tgttgatatc catttccgaa
    17941 ccgatattga ctggccctgg caaatcactc tgcatcagct tgataacacc atcaaccgca
    18001 tcagacacat agcacaacga agacgaaaat ttctcatcac cgttaattac caaatcctta
    18061 ttgtctagag cgttaataat aaaatctggt atcatgtgcc cctgattgat cggcaagcgc
    18121 ggaccataaa tccggaataa gcgaataatt ttagcattga taccatgaaa ctgccgataa
    18181 gtgttgacca ttgtttcagc aaaccgctta ccttcatcat aacacgctcg atcagatagc
    18241 tgatcaataa tacccaactc attttcggac accttagaac cattgtgctg ttcgcgactg
    18301 ccataaacca ctgatgacga aaagtgcagc aacttagctt gatattgacg agccatctct
    18361 aaaacctgga agacggctgt tgaattagcc aacaaattag ctaagcgatt ggccataaag
    18421 tcaaccggcg acatcggaca ggctaaatta taaatctctt gaataccctg aaatttaatt
    18481 ttaaaccgct ccaattctgg aagagccagt aaatctaaag gctcggtcat gtcatgacga
    18541 ataaatataa aatcaggatt agataagaga tgatcaatat tccgctcgtt gccgctggta
    18601 aaattatcta cgcaaataac tttattggct ttaatcaact catcgcataa atgagaccca
    18661 ataaaaccag cgccgccagt tactaaaaca ttcttcttgt caaaaatctt ttggactaac
    18721 ataaatttat ttagttaaaa tattgcttat ttaaattaag cgcggcagca ttaactccac
    18781 catcataggc ctgattatag acataccatg attgccgcaa atcaccgaaa gcatcaaaaa
    18841 tccgcacatg cggaccgcca cccggtccgg ccgaagtcac aatatccggc aaaccgttat
    18901 tatcaatatc gcttaaagcg acgctgaaac cttggcgaaa tttatcatta aaagccaaaa
    18961 aactggacaa taattgaccc ttagcgctaa aaatgcgaac ttgcggttgg cggccagctc
    19021 cagacgaaac aataatttca tcctgattag ctttagcccc ataattcaga ttaccggccg
    19081 ctacttggat accgccacga aaattactct cataagccat aaactcacct ttcaactggc
    19141 cggaaccact aaaaattcgc acatgcggac cgccacccgg tccggcaccg gtaataattt
    19201 catcaacatt atcgccatca atatcgccaa cagctaaact gacaccgccg gtaaaatttt
    19261 gattataagg gcgaaatttg gtttttaaaa caccttgagc cgtaaaaact ttaacttcat
    19321 tcttggcgcc gctgccagta gccacgatta tttcgccagc tttatcacta tcaatattgc
    19381 caactgctac cctgagaccg gcggtaaaat ctttgcgcca agcaataaat tgaccgacta
    19441 gcttgccttt ataattaaaa attttaaccg ctgactgacc gcctttatca ggaacgacaa
    19501 ctatctctgg tgatccatca ttatctaaat cgccgctggc taccctaagg ccataaggaa
    19561 atttaacacc aaaagcataa aattcagcta aagctttacc ggtagcatcc aaaatacgaa
    19621 tcggctgact actggaagaa gctggagcta ccaaaattcg attaacctga ttaactaact
    19681 gtaacgaagc atcgttgatg gccgaagcga catttaaccg gccgcggcct agttggccta
    19741 aataattcat attaaggcga gtaatgtcgt cagcgttatt gagcaatact ttgattagct
    19801 catcccgttt gagactagga ttttgctgca taattaaggc cagcgcgccg ctaaccatcg
    19861 gcacggaaac cgatgttcca gaccagtatc catcataata tttatccaac ggctggccat
    19921 caatctgttg attaggagca taaaccactg tactataaat actgaccccg ggcgcggcaa
    19981 tgtcaacgca gcgcgatcca tagctggaaa aattagtttt ctgatctaaa gcatcagttg
    20041 aagccacgcc gataaccata tttttatcgc catcatgaca ggctggatac atcggcaatt
    20101 tatccaatga atgaccatag ccgcccgatt cttcattgcc ggccgcggct actacaatta
    20161 ccccggctcg gtatgcccgt tcaatcgctt ccgctaagct ctggctataa ccataaccaa
    20221 caaaacttaa attaataata tccgctccat tattaatagc gtaatcaatg gcttgaatta
    20281 tcctaaaagt actgccgact ccttgatcat ccaaggcttt tagcggcatg atttgcgctc
    20341 gccaagtaac gccagctacg ccagccgcat tattgccggc cgccgcagcc acaccggcga
    20401 caattgtgcc atgaaaaaca ccatcctcgg taaaattagc ggcaaatttg ggattaggat
    20461 cggcagaatt attaacaaaa tcccagccgt taacatcgtc aatgaaaccg ttgccgtcat
    20521 catctttatg attattgggg atctcgcggc tattgcgcca aatattatct tttagatcag
    20581 gatgattaat ttgaacacca gaatcaataa tggcaataac aatatccggg ctctctcgcc
    20641 gctcttccca ggctatttgc gccttaatct tttgtaaata ccactgattg ctataatagc
    20701 tatctgacgg cgctaaagcc gcccgataaa tatcggctgc cacataacta tcgctggcca
    20761 tcgctggctg aacaatcaaa gccagccaac tcaagattaa cccaccccaa atataaaaat
    20821 tttttgaata tttattgagc tgcatgagct tcttgtaaaa tttttaaagt tgcttgagcg
    20881 gtcttagtcc agctaaaacc agccgcccgc ttcaaaccag ctttaattaa acgctgacgt
    20941 aaatcattat ttttaataac tacttcaatc ttggcggcta aatcaaccgg attagccgga
    21001 tcaaaatata aaaccgcctc gccgccaatt tctggcaaag aaccggtatt agacgataag
    21061 accggtgtgc ctgaggccat cgcttctaaa ataggcatac caaaaccttc ataccaagac
    21121 ggaaaaacaa aagcttgcgc ttggcgcaac aaactgggta aagccgtcat tggtgtccaa
    21181 cccaactcca cacaacgctt cgctaaaatc ggatcgctca gcaaattgct aacttgagca
    21241 aaatcagcac tacgctgacc ggctaaaact aatttaagcc gactccgcgg ttggtcttgg
    21301 caatattgac gaaagccatt gagcagaccg accatatttt ttttattctc cagccgtccg
    21361 atatataata aataaggctg ttcaatatta taatcggcta gaatgcgttg atcgtgatta
    21421 ggctcgctct ctggcttata aaagctgcta tcgtaaccat tataaacgac cttaattttg
    21481 gaccaatcgg ccgaaaaata acgttgaatc tcctgcttag taaattcgga aatagcaatc
    21541 agccgctggg ccttggctag cgcccactta gttgaccagc gcagataacg agcagcgtgg
    21601 cggccataaa gattgggaaa cgccataaaa ccaacatcgt gaatagtatt caccaactta
    21661 gccctagata gtaatggtaa aacatgcgct ggcacaaaca aaacatcagg cggacgaaca
    21721 atcatctcta aagttaaacg acccaatgtc cagaatcggc taaaaggcca attgagcacc
    21781 ttggatttaa aattagaata ctgctggcac aactcagcta agcctggctc taatggctta
    21841 ttagagtaaa gaatatagtg attatgccga tcaatagccg ccaattgctt aattaaatga
    21901 taagaatacc attcagtacc agttttattc ggccgattgg ccctggaagc atcaatacca
    21961 attaccatat atttaccgcg tggcaaaaat tgaacgctta tagctatcca gattctctaa
    22021 agcaataccg gttcccttaa ccacgcaagt tcgcggatca tcagctataa ataccggcac
    22081 atcggttgtt cgggcaatca attgatctaa attgcataat aaagctgtac caccgcttaa
    22141 aacaatacct ttttccataa tatccgaggc taattcaggc ggagtttcac gtaggatatt
    22201 tttggccgcc ccaataatcg cttctaactc atgctggatt gcctcggtaa catcatcgga
    22261 agtaacagta acagttttcg gcaaaccggt taaaatatcc cgccctttga tattaataga
    22321 taatttatca gtcaaataaa gcgcgctgcc gacaccgatc ttaatttctt cggctgtccg
    22381 ctcgccaata gccaaattat attttcgacg aatatattca ataatggcca aatcaatctt
    22441 attgccacca acccgtaccg aggtggcagt cactactccg cccaaagaaa taacagccat
    22501 ttcagccgtg ccaccgccga tatcaataat catgtgcccg gaggctgaac caaccggaat
    22561 gccggcacca atagccgccg cgactggttc tttaatcaga taggcagcct tggctccagc
    22621 gcctaaagtg gcatcaataa cagcccgacg ctcagttgaa gtaataccgg ccggcacagc
    22681 caccattact tccggccgaa acaaacgaat accgcctaag gccttattaa taaaataacg
    22741 aatcatgacc tcggttgtat gataatcagc aataacgccg tctttgagcg gcttaatggc
    22801 aataatcgta tccggcgtgc gaccaaccat atccttggcc tcatcaccaa cggccaaaat
    22861 ctttttatca gtcatggaaa cagccaccac gctaggctca ttaacaataa cacctttctt
    22921 gggcagatga actaaagtat aggttgttcc caaatcaata gcgatgcgat taataaacat
    22981 gaaaaatcaa aaatagatag aaaaactcag gccaaaagcc tgagtttagt atagcaaata
    23041 ccattaaaaa atcaactgat atcaaggcca caaagccacc tcgggaataa attctaaatt
    23101 atactgatgc ctgatctcgg ttttgacata ttcaatcaat tgacgcattt gttcagccgt
    23161 cgctccgccg ttattaacca aaaaattaga gtgctgccga ctgatttgag ctggaccaat
    23221 tgaatagcca gccaatccgg cctgactaat aataaaccca gctgacactt caccgcgact
    23281 aaccgctccc actgactgag ctgcctgtaa taatttcggg gccaacctag ccacctcatc
    23341 aacggccgga tttttaaaaa cgcagccggc gcttggtaat aacggatggc gttggcgtcg
    23401 ataagaccga tgctcatcgg ctattttctg cagctcagcc tgattaccaa tttctaaagc
    23461 taatttaact gaccaaatca atagctcagc acgatcttta aaaatactat gtcgataagc
    23521 atattggcaa tcttgagctg agatgacttc aatctgctta gatttaatat tataagccac
    23581 tacttcaata actcgatcgg ccaaccaacc gccaaaagcg ccggcattac cacggactgc
    23641 gccgccaacc tggccgggaa tacccgcagc ccactccaaa ccggctaatc catgatcaat
    23701 acaagctgta accagtttag acaagctatg accagctccc acttccacta ttttatcagt
    23761 aaagattggc tctggctccg ttaaagtaat aacaagacca gccactcctt gatcactgat
    23821 caataaatta ctaccaccgc ctaaaaaata aattggccaa tggtcttctg tcgcccaatc
    23881 aattgccgaa gttaattctt ctaaagaatt aattttggcc aaaaattgag ccggtccgcc
    23941 aatctgataa gtcgttagtg gtgctagtcg gacattttct tgaataaata ctggtcgctg
    24001 tttcatattt taaatttatt taactaaaag ttgctggcca atccgaaaaa tatcgccagc
    24061 ccccattaag acaatgactt cgccgctttt cgccgattga cgcaaatacg catccagtct
    24121 ggccatgtcc tcaaaataac tgacctcgtc tttcaacccc gctttagcgc gattaaccgt
    24181 aattcgctgg gctaattctt ggccggaaat accgccagct tcttcacggg ctgaaccata
    24241 aattttgaga ataccaactt catcagcctc attaaaacta ttaacaaaac catctaataa
    24301 agccagagta cggctaaatg tgtgcggatg aaaaaaaact cttagtttaa aatcagggta
    24361 ttgctggcgc aaagccgcta ggctagcctt aatttcagtc ggatggtgag cataatcatc
    24421 aataatggtg gcgccttgaa actgacctaa aacttctaag cgccgagcag taccaataaa
    24481 ttcagcgatt ccctcacgaa ttttatatag ttgcaaacct agctccacgc tagccgccaa
    24541 caccgctaaa gcattgctga tattatgacg gccgggcaaa ctggtactga aatcacccag
    24601 attgccagca ttactaaccg ctaatttact caatttttca tcttcctcag ctatatcgtc
    24661 taaatcaata cccattttaa ccttaaaata ctgccgacca ttgactgctt taagatcata
    24721 ggccaaataa tcggcggcat tatctaaggc atagctaata atccggccgc ggcaatgaac
    24781 cggcgccaag cgacgaatta acggatcgtc atagttggca attaaataac cgtcagttgg
    24841 tattttagct agaaaatcaa taaaagcttg ctgatagtcg gccggggttg gataaaaatc
    24901 agcatgatca taatcaatat tattgagcac caccattttt ggttggaaat aacgcaattt
    24961 attttggtat tcatcagcct caatcaccat taacgacgag cttcctaata aactagcccc
    25021 gttaaattgt ggcacccgtg aaccgaccaa gacatttggt tttaaaccac cttgccacag
    25081 ggtaaagccc aaccaagcgg aaaccgtagt cttgccatgg gaaccagcca cggcaatacc
    25141 ataatattga ttaaaaacat cgcctagggc ttcagcataa gtcaaagtcg gccggttatc
    25201 cagcgcccag gcgacttccg gattatcttg attataggcc gtagaataaa taaccaagtc
    25261 ggctgaatta agttgttcgt taaattgaaa cccggttcga acaataatat tatgatcagc
    25321 taaaatctga tcggtcataa aactctcggc tacatcacta cccgttatct ggtagccgcg
    25381 gctctgtaaa aattgggcca acatcgtcat gccaaccccc ttgataccga tacaataaat
    25441 atgtttaatc gctagccagt ccatattata aatattcttg aaaaacctga ataatctctg
    25501 actggctggc tattttgata gcctttggcc aattatttat taaatcaacc tgtttttgtt
    25561 tatccgctaa taattgttta agttgacgaa ccagctgacc gtcaaccaaa tctttttctg
    25621 gaacaacaat ggccgcgcca gcttccttaa tggcttgggc attatccaat tggtgggaag
    25681 ctggcatagg tataatcaaa ctaacttttt taagagcggc taactcaatt aaagtgccta
    25741 aaccagctcg agcgacaacg atatcagcag cttgataagc agccatcatc gctgcttggt
    25801 ttaaaaagtc gagcggttga taatgctctt gcggcttggc gcctaactta ccacgaccag
    25861 ttaagtgaat aatatcggct aattcagtta agccggataa ttgctgaacc actaaattat
    25921 taatacctgc cgccccggtc ccgccgccaa caatcaaaat aatcgaccgc tgaccgttaa
    25981 acggccacag caagctcggg ttgtcgccga ctgtttgcaa aatatccgga cgaatcggcg
    26041 cccccaacca aacagctttg cgaccataat cacggccgga cttagcaaaa gtcactaata
    26101 tttttttagc cagtggcgcc attaatttat tagccaaccc gggacgataa tccaattgtt
    26161 caataatcac tgggatacca taaaatttag ctgcccaagc aatcggcacg ctgacaaaac
    26221 tgccggcgct taaaatcaga tccggacgaa tccgattcaa aaaataattg gcttgcaaaa
    26281 aaccaataat taatcgaccg atgtctaaaa tattttgcca ggaccaatag cggcgcaact
    26341 tgccggcgct aatcggctgg aatttgatac ccgcttcttc aaccagctgg cgttccggtc
    26401 cccgatttgt tcccaaccaa ataaaatggc aatctgactt gtaatctcgt aaaaccttag
    26461 ctatagctaa gagcggtata accgagccgg ctgtaccacc accagttaat aaaattatct
    26521 ttttatttga tcgagttttg atcataactt aggtaaattg tctggcagac gaacttgtcg
    26581 agaaatatta gctaatatcc ctaaaccaaa taataaagac attaatgatg acccgccata
    26641 actaatcagc ggcaaaggca cgccagtcat tggaaataaa tttatagtac cgccaaggtt
    26701 aataaaaacc tgaatcatta tcccgctaac aataccacca gctaatattt gaccatataa
    26761 atcaggagcc gctttagcta actggaagcc ccgataaaat aaagcgaaat aaagcaatac
    26821 taaaaagact gaaaaccacc aacccaattc ttcagcaata atcggaaaaa taaaatcatt
    26881 ttgcacttcc ggcaaatata aatatttttg tcggctctcg cccaatccgc gcccccaaaa
    26941 accaccggag ccgatagcaa ttaacgattg attaacctga taacaactct tatccggact
    27001 ataaccaggc tttaagac
//
LOCUS       DAOPAI010000007        24205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_2227_length_24205_cov_4.546957, whole genome shotgun sequence.
ACCESSION   DAOPAI010000007 DAOPAI010000000
VERSION     DAOPAI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24205
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2227_length_24205_cov_4.546957"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /gene="serS"
                     /locus_tag="PKV49_01590"
     CDS             <1..856
                     /gene="serS"
                     /locus_tag="PKV49_01590"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPL22339.1"
                     /translation="LDFERAAKVTGSKFYYTSNDLVWLELALIQYTLSVIAKHGFQVM
                     TTPDLAKTSIIERLGYNPRGESTQIYNIANNDLSLIGTAEITLGGYHQGEILKGEQLP
                     LRYAALSQCFRTEAGSYSKFAKGIFRVHQFNKVEMFVYCRPEDSAQEHQLLLAIEKEI
                     FSGLEIPFRVIDHCTADLGAPAARTFDLEAWLPGKPNQDGQAGDWAEITSVSNCTDYQ
                     ARGLDIKYQAADGQKGLVHTLNGTGLALTRAMIAIVENHQQADGRIKLPAILKSFIPV
                     DLSSMFFA"
     gene            863..1702
                     /locus_tag="PKV49_01595"
     CDS             863..1702
                     /locus_tag="PKV49_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HPL22340.1"
                     /translation="MKARPQPKKSVVLLNQRPKAASLPARRISNSPKGVNWRKIGRGA
                     VLIAAIVLIGGLIYGLLGSPYFRASSVSIDGAQLLDANILRQQSETALQGHVWQFWSG
                     NFWLASSKRYCRPLQEYNLVSCRLKRQWPNKLTLKIEEEPVVAIWQENNWYYWVDRFG
                     RVVKQESPNAASAKLYPVIKSRDTLVTERQVAINPDFWPLIIASQTNWLGSVPHNFIF
                     SQQEPNSIQAVLDNQQIIKLTMRESLDNQLRLWQVGQSKFAQQLSQAKVIDLRYGDRI
                     IFQ"
     gene            complement(2047..2619)
                     /locus_tag="PKV49_01600"
     CDS             complement(2047..2619)
                     /locus_tag="PKV49_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22341.1"
                     /translation="MTYHRSKPGFGPLEAILILSTLSLVISAGFTYWSINQAKQRDQI
                     RIANLKIIQSAINQYFNQYREFPAGDHDVGNWDLGYRNASDQRFIAPLVEKGFLPANF
                     AADPISKYDENKGIDYTFRYQVYQDDGQYCPRGQGQFYVLGIRDLETDSGGLNNKASQ
                     FTGSGFLCKNRNWQDEFDYVVGAFANPIKK"
     gene            complement(2625..3887)
                     /locus_tag="PKV49_01605"
     CDS             complement(2625..3887)
                     /locus_tag="PKV49_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPL22342.1"
                     /translation="MKNCRYQKILSGSLIIISLSLFLCLVAYHPQPARGSYEDEVNQL
                     NSQINAKKNSLTKLRDQQRYYQQLVAEKQSEKATLNNQLTILDSKIAEAELDIQGTEL
                     EMEQTNLELRSLELEIADKDQSIDQNKTKLGNLLKTINQQGSKSALEIILLNDSFSEF
                     LNEIKYLNDISSGVRDQLEDLRKLKNSLEEQQAALDIKHQQLAELQKVLADKRNSLDQ
                     EKQDKSFILEQTQLSESQYQNLLAAAKQEQAQANAEISSLEKSVRQKLALIEANKPKT
                     NQKKSSSNSSSGLIWPVPKNYVSTYFHDPEYPFRYLFEHPAIDIRASQGTAVVAASNG
                     YVAAAKDAGMGYSYIMIVHDNGLATVYGHISRIATKEDAYVTAGQVIGYSGGMPGTPG
                     AGKLTTGPHLHFEVRLNGIPVDPLAYLP"
     gene            complement(3910..5385)
                     /gene="gltX"
                     /locus_tag="PKV49_01610"
     CDS             complement(3910..5385)
                     /gene="gltX"
                     /locus_tag="PKV49_01610"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPL22343.1"
                     /translation="MPDARLRLAPSPTGFLHLGNLRTAIFGYLTAKQLRGHFILRIED
                     TDNKRRVQGAEDSLIKVLAQLGIKFDEGPHIGGGYGPYVQTERLDIYQHYAQKLIDSG
                     KAYYCFCSQERLEKLRADQTASHLPPRYDRACRRLSAKEIAQRLAAGEKYVIRQKMPT
                     KGEITVIDALRGAITFAASDLDDQVLMKSNGIPTYQLASVIDDHLMEISHVTRGAEWL
                     PSFPKNILLYQAFGWTPPEFIHLPLILNKDGGKLSKRQGDVFVEQFLDKGYLPLALIN
                     FCVLLGWHPQGDQEIFTSWTDLISQFDYRKINSSPAIFDLDKLNFLNGYHLRQLPTEQ
                     LAELLIPYLTENQALTTEKFKKEFNFSLKIAKLYQERLKNLNEIGELSRNLYLDQLNY
                     DPALLVWKKSTPAATIEALKTVINFLKNYPQADWETAKLQTATMDFLQKNNLSNGDHL
                     WPWRVALSGLAASPSPFELADILGQTVTIKRLRRALQSMQV"
     gene            5517..5909
                     /locus_tag="PKV49_01615"
     CDS             5517..5909
                     /locus_tag="PKV49_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22344.1"
                     /translation="MSRICTLIILLTISLMLAGQSTGRVNFKYQSRCYPDVVWYGTSS
                     GLYTAHHCAGLTDRNQIQSRNNEIVIKNIIDLYEELGSSYNTVLVAIYDGQYYQTFSL
                     DTGHVFRVSPPQAITRLAWDKIKKNGLK"
     gene            complement(5964..6707)
                     /locus_tag="PKV49_01620"
     CDS             complement(5964..6707)
                     /locus_tag="PKV49_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPL22345.1"
                     /translation="MSTIKILGVRLDNLTGQALQQRLAVYLASQQNHLIVTVNPEFTL
                     AAQKNQTFSNILNQADLSLPDGFGLSLAALTYGQKLIRHTGADLTADLLIQAEKNGWP
                     IAIINWRGGLSPAAILRPALLTKFPKLRCLIEDCGRNGYLGQRQLSRLNDFQPRLVFC
                     SLGAPYQEYFLAKHQTTWPSCRIMIGIGGSFDFIIGRIKRAPQLWRRCGLEWLWRLIQ
                     QPWRWRRIWNAVAVFSWRFIIWRYKNKNR"
     gene            complement(6694..7437)
                     /locus_tag="PKV49_01625"
     CDS             complement(6694..7437)
                     /locus_tag="PKV49_01625"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPL22346.1"
                     /translation="MKTNKSVQNSYSVIRQALQKLANPAKAAILGRFFKTGPGEYGQG
                     DIFWGITVPEQRTVAKRFANLSLDIIKKMLADPIHEVRLTAGLILVDQCRHNPSQHQD
                     YLDFYLNNIASFNNWDLIDLTAPKIIGQYLVNNQNKKLLLLKELRRNDNLWARRAAMV
                     ANLTLIKHGDYRTALRMAPQYLKERHDLLHKATGWILREVGKRNQTALLNFLDHWASR
                     MPRVMLRYSLEKLPAPLRQKYLQIRYVNH"
     gene            complement(7434..7763)
                     /locus_tag="PKV49_01630"
     CDS             complement(7434..7763)
                     /locus_tag="PKV49_01630"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPL22347.1"
                     /translation="MKKIIPPITPLASLVYSVTQQIPRGRLSTYGAIAQAIGRPRAAR
                     AVGQALNRNPYWPVVPCQRVVGSTGQLTGFATGLNKKRQLLQQEGIVIKNNCVTDLAR
                     VFYQPHL"
     gene            complement(7841..8149)
                     /locus_tag="PKV49_01635"
     CDS             complement(7841..8149)
                     /locus_tag="PKV49_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22348.1"
                     /translation="MVNLVPLLVNVYLSGKEKACKQALQIIAAGRLSYIRRIISRALL
                     AHICVNEDDETVEYCFQRLKEIISGFYADNFESSLTKEEIAAIRSWLETSFSARPRAD
                     "
     gene            8625..11282
                     /locus_tag="PKV49_01640"
     CDS             8625..11282
                     /locus_tag="PKV49_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPL22349.1"
                     /translation="MDMLDKFTQKSQEAIINAQMVAQQFGQEFIGALHLLLALLQQDE
                     SLVRPIIEAQKIDPDLVASQTEAELDKLPKSDRAEVAEGVVQGTAEASMVIERARKEA
                     KNLDDEYIATEHLLLAIVGIKSKAQSILLKVGLGYEGLLKALIELRGSQAAGSTDAEK
                     KYRILQKYAINLTDLARQKKLDPVIGRDEEIRRIMQVLSRRTKNNPVLIGEAGTGKTA
                     IVEGLAQRIIAGDVPETLKGKELVSLDLGALIAGAKFRGEFEDRLKSVIKEVQKSNGQ
                     VILFVDELHTIVGAGASEGSMDASNMLKPALARGEIKLVGATTTKEYQKYIEKDAALE
                     RRFQPIFVEEPSVEDAIAILRGIKEKYEVHHGVRITDDALIAAVKLSARYITDRFLPD
                     KAVDLIDEATSSLRMEIDSSPDELDGLKREIRRLEIMKAGLLKEDNKEKVRKVNRDLE
                     NLKEKAKQLELHWSNEKEIISKIRQFKKDIDELRAQAEIIERRGDDLTKVAEIRYSRI
                     PDLEKAIEMERQNLLKIQSNGQRILKEEVDEEDIAKVVARWTGIPIARMLETEAQKLT
                     KIEDYLKARVVGQVEAIVAVANAIRRSRAGISEEKKPIGSFLFVGPTGVGKTELAKTL
                     AEFMFDDENALIRLDMSEFMEKHSVSKILGAPAGYIGYDDGSQLIDRVRRRPYSVILF
                     DEIEKAHPEVFNILLQILDDGRLTDAKGRVINFKNTIIIMTSNLGNEVIKDYSIGFYD
                     GSDAKKLAEIREDEMKDKIDNILKQHFKLEFLNRIDEIVIFKSLSREALGNIVELELQ
                     KVSRRLAIKGIKFKVTAKVKRFLADQGYDITFGARPLKRVIQNQLLDELALQIIEGKI
                     KEGDMINADLSSGNKIIFKTGAELKNHQD"
     gene            11287..12834
                     /locus_tag="PKV49_01645"
     CDS             11287..12834
                     /locus_tag="PKV49_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22350.1"
                     /translation="MKSLTSKVLLIIGLIASSWLVNGGRAQAADFNPNLIITDQEIMD
                     SAAMNLGEIQNFLEDRGSFLATYRTTDYYGVVRSAAEIIYNAAVNSYDCSGIEVNNPQ
                     DIEEAKIKCVKVAINPRFLIVLLQKEQSLIDSKGPRQSQLDWATGYGCPDNAACGERW
                     RGFGKQVNAAALQFYDYMVNPRYYRYQVGQTYTITNTGRDPMIVVPANRATAALYNYT
                     PHVYNGNYNFWNIWQRYFTQVYPDGSLLQAAGQETVYLIQSGRKRPFSSRGALVSRFN
                     PNKIVIVRPEDLDSYPEGAPIKFQQYALVQAPSGAVYLLVDDKRRGFTSQQAFAKMGF
                     NRQEVIKGSWEELAAYKESTPITVTSTYPTGALLQDKKTGGVYWVEEGNKAPLLDRAT
                     LAAKFAKRRIIPADQKELDAYKTVDPVNLDNGSLIKLDSDPNVYLLDHGQRRLIASER
                     IFNHLGYSWANIITISSRHFSLYNQGPIIEDNLPADYGQPMVIPTPDNLVPTVTATTT
                     SSTSTNP"
     gene            12839..13639
                     /locus_tag="PKV49_01650"
     CDS             12839..13639
                     /locus_tag="PKV49_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HPL22351.1"
                     /translation="MALAFAGLVPHSPLLAPNIGKEHRAQLGKTLEAYSLLSKNLATT
                     QPDAIVIISAHAASGPDWWNANVCLNYQVDLSLFGDLLTQGQWSGALGLAEELQNFLG
                     QTEQLRFYSQPQLDYGTAVPLLLLGEDLKTVPILPIGYIDNQPERQAEFGRRLGEFLR
                     QWPGKAALIGSGDLAHCLGANQPSGLQARGHKFDQRLQEALRQNDSQALLELSMDKAN
                     ETGQCAWLAYLTLQAALNGQPGSWQNLAYEAPFGVGGAVWQWQPASAD"
     gene            complement(13626..13919)
                     /locus_tag="PKV49_01655"
     CDS             complement(13626..13919)
                     /locus_tag="PKV49_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22352.1"
                     /translation="MPRDIRQFTDISQLIKRPRPIIKPTAYPWQQLALEVIERLRIPN
                     FKRNSVFQACRQFPESVIRQAVIDTQELCQQGSRWQYFFKILGNYRQDQFNRR"
     gene            13974..14450
                     /gene="def"
                     /locus_tag="PKV49_01660"
     CDS             13974..14450
                     /gene="def"
                     /locus_tag="PKV49_01660"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPL22353.1"
                     /translation="MDQKQISSGRVRRLVINPDSRLRRVAQTVDLAMLQDKKFQELMV
                     DMAKTMLVKDGVGLAAPQIGRNWRLIVVNTDEGVWPMINPVLSDLSSATIVEEEGCLS
                     VPNVFGPVRRSKELICHYINDLGQEVHLPAKGALARVIQHEVDHLDGVLFIDHLSQ"
     gene            14453..15388
                     /gene="fmt"
                     /locus_tag="PKV49_01665"
     CDS             14453..15388
                     /gene="fmt"
                     /locus_tag="PKV49_01665"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPL22354.1"
                     /translation="MASPKVKILLAGSPSLAVPAFQAIVNDQRFQVLGLLTQPDQPAG
                     RGLKLTPPPTKVWALKNGLTVWQPEKLKTWQSALVNIKPDMVVVIAYGQLIPQALLEI
                     PPQGWVNVHGSLLPKYRGAGVLSAPIINGDQQTGISIMKLVKELDAGPVIAQVKINLA
                     SDETMGSLASKIGQVAADILPDTLIGYIERRLIPQKQNDSQASYVGLVATADAKIDWH
                     KSALEIERLVRAMQPVPGAWTTWRDQRLKIIEATVSDQSWLNPGKVGLIDGQLAIGSG
                     QGALIINRLQLAGRQAVSGNDFLIGQADVLGEYLA"
     gene            15440..15511
                     /locus_tag="PKV49_01670"
     tRNA            15440..15511
                     /locus_tag="PKV49_01670"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15473..15475,aa:Glu,seq:ctc)
     gene            15556..16935
                     /locus_tag="PKV49_01675"
     CDS             15556..16935
                     /locus_tag="PKV49_01675"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPL22355.1"
                     /translation="MTDWSMFKQDELNNPNNISPHPFSASSNQQSSKKKIWLILLIAV
                     ILITVGGFFIYQAYAQSPERIIKKMFINLSKVKTWQYSGEIKTEGNLGQSLTNQANLS
                     LMAPLNQDKKNSQTVVKFNGLMDIQKINDPQILLALEVKTDALTSANISFGLETRFIN
                     QIIYAKLSTVPYLGFFDLSSLKDQWLKVDVKTLKQQIQASKLKKEPDNNQANSQLSPE
                     QISKLITAIQRANILTITDSKLLDAKTYKTSNGGKHYQYQFILNPEGIKLFLVELVKI
                     VNNKELTSIEADKLYEKIYGQHELLAGKLLISQKDLLPRQISFDVITKTADKTKVLWK
                     TSQTISFSDFNKPVSIDVPTTTKTLMELLSGFAQKLPAHQYNSSSTTTPTSIMPHIKH
                     SLNDSDNDGLSDNDELNIYSTDFLNPDSDGDGYSDGDEVKGGYNPNGSGKLLIDDLTV
                     DKSAPFLIK"
     gene            16983..17168
                     /gene="secE"
                     /locus_tag="PKV49_01680"
     CDS             16983..17168
                     /gene="secE"
                     /locus_tag="PKV49_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPL22356.1"
                     /translation="MEKITAYFRSAWAEIKKVTWPTKKETYNYTLLVIGISLGTAIFL
                     GGIDALFAAGLKLLIKQ"
     gene            17191..17736
                     /gene="nusG"
                     /locus_tag="PKV49_01685"
     CDS             17191..17736
                     /gene="nusG"
                     /locus_tag="PKV49_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPL22357.1"
                     /translation="MAKQTLNQGRRWYVLHTYSGYEENVAQNIKQRIESMDMEDKIFN
                     VMIPTEKKIKIVNGKRKVYEEKIFPGYILVEMIVTDDSWYSIRNTPNVTGFVGTGTVP
                     TPIDEAEIKELQKRMGLDEPKFKIDVSVGSPVRIIDGPFKNLEGKVTEVDENRGKVKL
                     LISMFGRETPVELDFLQINKL"
     gene            17804..18229
                     /gene="rplK"
                     /locus_tag="PKV49_01690"
     CDS             17804..18229
                     /gene="rplK"
                     /locus_tag="PKV49_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPL22358.1"
                     /translation="MAKKIVTQIKLQIKGGQANPAPPVSPALGQHGLNIADFCSKFND
                     ATRDKIGDVVPVVITVYADRSFDFITKTAPASELIKKAAKINKGSSKALKEKVGSLTT
                     AQLQEIAETKISDLNARDVEAAMKIIAGTARQMGIEIKD"
     gene            18294..19010
                     /gene="rplA"
                     /locus_tag="PKV49_01695"
     CDS             18294..19010
                     /gene="rplA"
                     /locus_tag="PKV49_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPL22359.1"
                     /translation="MAKKVQKNKTAKQGKAQAAVRQLVDKTKTYVLAEALELVKKTSV
                     TKFDASVEVHLRLGIDPRKGDQQIRGAVSLPHGTGKTVKVAAFVSPENEAAVREAGAD
                     LVGGDELIEEIRKTEKTDFQVAVAEPALMRNLAVISKILGTRGLMPSPKNETVTTDPA
                     KAVTELKKGKVSFKNDDTANLHVAIGKVSFSVEQLADNFQSLIEIVKKNKPSAAKGTY
                     IKSVTICSSMGPGIRVLLGQ"
     gene            complement(19077..19388)
                     /locus_tag="PKV49_01700"
     CDS             complement(19077..19388)
                     /locus_tag="PKV49_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPL22360.1"
                     /translation="MNRRLLQRKVNKYLQEPAAKLDLHGHSRREAEAALIGFFDYWIK
                     QGGGVKLLIITGQGWHSADGHGVLKDFTAAWLQARGYRQRTAKPQFGGNGAIEVDLPI
                     T"
     gene            19478..20014
                     /locus_tag="PKV49_01705"
     CDS             19478..20014
                     /locus_tag="PKV49_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPL22361.1"
                     /translation="MPSRPSKLEYYMNIAGEVAGRSTCYRAKHGAIIVRDDQIISTGY
                     LGSPRKTKDCWERGNCLREMLKVPHGHRYELCRSVHAEQNAIINAARAGASLLGSTIY
                     LYSTVIRNGQEKPQDAYPCFICKKMIINAGIEKLVGRQADGSFKVYEISDWVKDWQER
                     DMLDDNEQYGMTVNIEQT"
     gene            20035..20589
                     /locus_tag="PKV49_01710"
     CDS             20035..20589
                     /locus_tag="PKV49_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPL22362.1"
                     /translation="MNKLIIGLTGQLAAGKTTVAKYLAEKYGGKVYGFSGPLRDIVDR
                     LTLPQTRENMANLSSILRRQFGQDIILKAIMRDVQNDPCPFILLEGMRRYGDFVAFRE
                     FPNFHLVAVLAEQKLRHQRLTKRGQNPDDASKTLEQFKIDEQGEAEQDIPRAIAEADF
                     QIDNNGDLAALYEQAENIYRQLAG"
     gene            20607..21191
                     /locus_tag="PKV49_01715"
     CDS             20607..21191
                     /locus_tag="PKV49_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22363.1"
                     /translation="MAGKLIVLYGINNLGKSTQANLLVNRLRDAGYAASYLKYPIYEL
                     APSGPLLNAYLRKGNPLQLSPREAQIIYALNRFQYQPELVRQLAADEWIIAEDYTGTG
                     LAWGWGAGVKLNFLEAINEGLLKPDLALWLDGQRFVSGIEQGHKHENNNQLMDKVREC
                     HQQLAERYGWPRFNANQSIDNLNDQLWRAVQALF"
     gene            21216..22709
                     /gene="gatB"
                     /locus_tag="PKV49_01720"
     CDS             21216..22709
                     /gene="gatB"
                     /locus_tag="PKV49_01720"
                     /inference="COORDINATES: protein motif:HMM:NF004014.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPL22364.1"
                     /translation="MEQLMPVIGLEIHTELKTKSKMFCRCDNNTEEQAANTLVCPICL
                     GHPGTLPQPNRQAVHFGLLLAKALNCQINQHSRFARKNYFYPDLPKGYQITQADTPLG
                     YNGKLSIDGRDIFITRIHLEEDTGKLAHDKNNQHSLVDFNRAGTPLIELVTGPVITSA
                     QEARRFGQEFQKLLRYLGISDANMEKAEMRCEANISLQTAGRYQIDGATISGLAGYQL
                     NNKVEIKNLNSFKALERAIDYEIKRQSEVLSSGGQVAAETRGWDEADNATKAQRVKEA
                     TADYRYFTEPDIPPLVIDDHWLAIAASEVDKIELPLAKAQRWQVDYQLSADNANLLTS
                     WPATATYVDQVLVLATQARPKAAAIITKVAINWLTSEVAKHCRLDNLANSPINPEHLV
                     DLAIAITDNKINSTAGQAVLAELINNHNLSVAEVIEKLDLGQVDNSDELAKLAQVIIA
                     EYPQQVSEYRAGKTPLMQFFVGQLMRRSGGKANPQLAREVLEKELNR"
     gene            complement(22698..23117)
                     /locus_tag="PKV49_01725"
     CDS             complement(22698..23117)
                     /locus_tag="PKV49_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPL22365.1"
                     /translation="MHTKRVRAIIIDHGKVLTIKRTKPDQTYWVIPGGEVESGETNQQ
                     ALIREIKEELGLIIEPRELLIEVSSTKPETFGQMEYFYACNIISGTLGSGDGPEFQKD
                     SAYVGRFDFEWLSAQDLTDLDLRPNTVKNWLRQSLSI"
     gene            complement(23121..23609)
                     /locus_tag="PKV49_01730"
     CDS             complement(23121..23609)
                     /locus_tag="PKV49_01730"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HPL22366.1"
                     /translation="MEILPILLIAFITVIILKYLKSRQLTPAKDQKIIYYKNKYFLTD
                     AEKNFFFALKPIADRHNLFIFSKVRLIDLFFVPHYNRSSRARIIQKHVDFVLCEPRNI
                     NPVCAIELDDSSHKWQSRIERDDFINQVFNSAGLPLIRIKNAYSYNPRDIEAKIISTI
                     NR"
     gene            complement(23659..>24205)
                     /locus_tag="PKV49_01735"
     CDS             complement(23659..>24205)
                     /locus_tag="PKV49_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002784784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22367.1"
                     /translation="KNGHYNIIKGEGYVDIPEIKSEVIIYASKLDFNLDSSLIGNSEM
                     QHLDFAYASSLIRTFMEDSTLVLTIRGRKYTPDFNFFVGKQQINVSGVQTEVDAGYEG
                     RDQIVLIEAKNKNTKDIIIRQLYYPFRQWQTHTKKKVITLFFEKDDKTNIYSIWQFVF
                     NDVDDYNSIKLKKMGRFKIAS"
BASE COUNT         6965 a         4691 c         5484 g         7065 t
ORIGIN      
        1 gctggatttt gaacgagcgg ctaaagtgac cgggtccaaa ttctattata ctagcaatga
       61 tttagtttgg ttagagttag ctttaattca atataccctg tccgttatcg ctaaacatgg
      121 ttttcaagtg atgaccactc ctgatctggc taaaaccagt attattgagc gcttgggtta
      181 taatccgcgc ggcgaatcga ctcagattta taatattgcc aataatgatt tgagtttgat
      241 tggtacagct gaaattaccc taggggggta tcaccaaggc gagattttaa agggcgagca
      301 attaccattg cgttatgctg ctttatccca gtgttttcgg accgaggcgg ggagttatag
      361 taagtttgct aaaggtattt tccgtgttca tcaatttaat aaagtggaaa tgttcgtcta
      421 ttgccggccg gaagatagtg ctcaggaaca tcagctatta ttagcgattg agaaagaaat
      481 ttttagcggt ttggagattc cgtttcgggt tattgaccat tgtaccgctg atttgggcgc
      541 tccagcagct cggacttttg atttggaggc ttggctgcct ggcaagccca atcaagatgg
      601 ccaagctggc gactgggcgg agataacatc agtgtctaat tgcaccgatt atcaagctcg
      661 tggtttggat attaagtatc aagctgccga tggacaaaaa ggtttagttc acactcttaa
      721 tggaaccgga ttagctttga ctcgcgccat gattgctatt gtagaaaatc atcagcaagc
      781 cgatggtcgg attaaattac cagctattct taaatcattt ataccagtag atttatcgtc
      841 aatgtttttt gcttaaagca ttatgaaggc tcgccctcag cccaagaaaa gtgttgttct
      901 cttaaatcag cgccccaagg cagcttcatt gccggcgcgg cgaatatcca acagccccaa
      961 aggggttaat tggcgtaaaa tcggtcgggg agccgtgttg atagcggcca ttgtcctaat
     1021 tggcggacta atttatggac tacttggttc accttatttt agggcttcta gcgtttccat
     1081 agatggcgcc cagctcttag atgcaaatat actgcgtcaa cagtcggaaa cagcgcttca
     1141 gggtcatgtt tggcagtttt ggtcaggtaa tttttggtta gcttcaagta agcgatattg
     1201 ccgtccgttg caagagtata atttagttag ttgtcgtcta aagcggcaat ggccgaacaa
     1261 gttgacgctt aagattgaag aagagccagt ggtagcaatc tggcaagaaa ataactggta
     1321 ttactgggtt gatcggtttg gacgggtagt taagcaagaa tcgcccaacg ccgctagtgc
     1381 taagctttat ccggtgatta aaagccgaga caccttagtg actgagcggc aagtagccat
     1441 taatcccgat ttctggccat taattattgc tagtcagacc aactggctag gttcagtacc
     1501 tcataatttt atttttagtc agcaagagcc taactctatt caggcggttt tagataatca
     1561 gcaaattatc aaactgacca tgcgagagag cctagataat caattaaggc tatggcaagt
     1621 cggccaaagc aagtttgccc agcagttgag tcaagccaaa gtgattgatt tgcgctacgg
     1681 cgatcggatt atttttcaat aataaataat aagtttaata aataatatgg atgaacaaat
     1741 cttcaagcta gtcttcttct tagccgttct cgttttctgt atcttagtgg tcggtatttt
     1801 tctattgctc tgcaaaattt tattactctt cagcccagaa ctgcatttaa tgggtatggt
     1861 tttgacttac ccataaagca cactagagga aacagatcag ggccactcta gctactatgt
     1921 cgtataagat gtattgtgcg acattatgac tagcgctccc cgcagcggct ttgagcttat
     1981 aataaaaaaa tcggcaacta tcgccgattc tttattgatt ctatttaatt atcctttagg
     2041 taatatttat ttttttattg gattagcaaa agcgccaacc acataatcaa attcgtcttg
     2101 ccaattacga tttttgcata agaacccgct accggtaaat tggctagctt tattatttaa
     2161 accgccactg tcagtttcta aatcgcgaat gcctagaaca taaaattggc cttggccacg
     2221 tgggcaatat tgaccatcat cttggtagac ttgatagcgg aatgtatagt caatcccctt
     2281 gttttcatca tatttagaaa tcggatcagc cgcaaaattg gccggcaaaa atcctttttc
     2341 cactaatggc gcgataaaac gttgatcgct agcattacga tagcccaaat cccaattgcc
     2401 aacatcatga tcaccggctg gaaattcacg gtattgattg aaatattgat tgatagctga
     2461 ttggattatt tttagattgg caattctaat ttggtcgcgc tgtttggctt gattgattga
     2521 ccaataagta aagccggcac tgatgaccag cgataaggtg cttaagatta aaatagcttc
     2581 taacggccca aatccgggct tggaacgatg ataagtcatg gtgattaagg tagataagct
     2641 aaaggatcaa ctggaatgcc gttgagacgg acttcaaaat gcaaatgcgg tccggtggtt
     2701 aatttaccgg caccaggcgt gccaggcatg ccgccgctgt agccaatcac ttggccggcg
     2761 gtcacatagg cgtcttcttt agtggcaatc ctagaaatat ggccgtaaac cgtggctagg
     2821 ccattatcgt gaacaatcat aatatagcta taacccatgc cggcatcttt agcggcggcc
     2881 acatagccat tactggcggc cactacggcc gttccttggc tggcgcggat atcaatggcc
     2941 gggtgctcaa aaaggtagcg gaaaggatat tctggatcat gaaaataagt gctaacgtaa
     3001 ttttttggca ctggccaaat tagaccggat gaactattgc tactagattt tttttgattg
     3061 gtcttaggtt tattagcttc aatcagggcc aatttttgcc ggacgctttt ttccaaacta
     3121 ctgatttcag cattagcttg agcctgttct tgtttcgccg ccgccagcag attttgatac
     3181 tgactttctg acagctgagt ctgctcgaga ataaagcttt tatcttgttt ttcttggtct
     3241 aatgaatttc ttttgtcagc tagaactttt tgcaattcag ccaattgctg atgcttgata
     3301 tctaaggcgg cttgttgttc ttccagagaa tttttaagtt tccgcaaatc ttctaattga
     3361 tcgcgaacac cgctactaat atcgttaaga tacttgattt catttaagaa ttcggaaaaa
     3421 gaatcgttta ataagataat ctccagagcg cttttgctcc cctgctgatt aatggttttg
     3481 agcagattac cgagcttggt tttattttgg tcaattgatt ggtctttatc ggcaatttct
     3541 agctctaggc tgcgtaattc taaattggtt tgttccattt ccagctcagt gccctgtata
     3601 tctaattcag cttcagcgat tttactatcc agaatggtca gttggttatt gagagtggct
     3661 ttttcacttt gtttttcagc cactaattgc tgataatagc gttgttggtc gcgcagcttg
     3721 gttagggagt tttttttagc gttaatttgg ctattaagtt gattaacctc gtcttcgtag
     3781 ctgccgcgcg ctggctgtgg atgataagca accaagcata aaaataaaga caaactaatg
     3841 attattaggc tgcctgataa tattttttga tagcgacagt ttttcatggt taaattatac
     3901 tattttttat taaacttgca tagactgcag ggcgcgtcgt agacgtttga tagttactgt
     3961 ttgaccaaga atgtcagcta attcaaatgg actggggctg gcagccaggc cactcagggc
     4021 aactcgccaa ggccagagat gatcgccgtt agataaatta ttcttttgca gaaaatccat
     4081 agtagctgtc tgtagcttag cggtttccca atcggcttgg ggataatttt ttaaaaaatt
     4141 aataaccgtt tttagggcct caatagtggc ggctggcgtt gattttttcc aaactaatag
     4201 agctgggtca taattaagct gatccagata taggttgcga ctcaactccc cgatttcatt
     4261 cagattttta agtcgctctt gatatagttt agctattttt aggctaaaat taaattcttt
     4321 tttgaatttt tcagtagtta atgcctgatt ttctgttaaa tatgggatta ataattcagc
     4381 taattgttca gtcggcagct ggcgtaagtg atagccatta agaaaattga gtttatctag
     4441 gtcaaaaata gccggactgc tattgatttt acgataatca aattggctga ttaagtcggt
     4501 ccagctagtg aagatttctt gatcgccttg cggatgccaa cccaaaagaa cgcagaaatt
     4561 aattaaagct agcggcagat agcctttgtc caaaaactgt tcaacaaaaa catcgccttg
     4621 gcgtttactc agtttgccgc catctttatt aagtattagt ggtagatgaa tgaattcggg
     4681 tggtgtccag ccaaaagcct gataaagtag aatattttta ggaaaagacg gcagccactc
     4741 agccccgcgg gtaacatgac tgatttccat taagtgatca tcaataacgc tagccagttg
     4801 ataggtcgga atgccattag acttcatcaa aacctgatca tcaaggtcac tggccgcaaa
     4861 agtgatggcg ccgcgtaaag catcaatgac ggttatttcg cctttggttg gcattttttg
     4921 tcgaataaca tatttttcac cagccgctaa gcgctgagcg atctctttgg cgctgagtct
     4981 gcggcaagct cgatcatagc gcggcggtaa atgactggcc gtttggtcgg cgcgcaattt
     5041 ttctaagcgc tcttgactac aaaagcaata ataagcttta ccgctatcaa ttagtttttg
     5101 ggcgtaatgt tgataaatat cgaggcgttc agtttggaca taagggccat aaccgccgcc
     5161 aatatgcgga ccttcatcaa atttaatgcc aagttgagcc agtactttaa tcaaactatc
     5221 ttcggctcct tggactcgtc gcttgttgtc ggtatcttca attcgtaaaa taaaatggcc
     5281 gcggagttgc ttggcggtta aataaccaaa aatagcagtg cgtaaattgc ctagatgaag
     5341 aaaaccggtt ggtgatggag ctaagcgcaa gcgagcgtca ggcataggga ataaataggg
     5401 ttaataacca ttatgttaat tatagctaat tgactaacca ttggcaaact taattgacaa
     5461 aactattggt tttagtaata tatggttatt ctgtcaaaaa aaaggaggtt aaaaaaatga
     5521 gcagaatttg tacattaata atcctgttaa ctatcagctt aatgttagct ggtcagtcaa
     5581 caggtcgcgt taattttaaa taccagtcac ggtgctatcc agatgtagtc tggtatggca
     5641 ccagtagcgg tctgtatacg gcgcaccatt gtgccggact aactgaccgt aaccaaatac
     5701 aatctcgtaa caatgagatt gttattaaaa atatcattga tttgtatgaa gaacttggct
     5761 cttcatacaa tactgtccta gtagccattt atgatggcca gtattaccag acctttagtc
     5821 tggacactgg ccatgtattt cgggtcagcc caccccaagc tattaccaga ttagcttggg
     5881 acaaaatcaa aaagaatggg cttaaatagt ccattcacct tttctccata ttctcaaaac
     5941 tttaccgcta accctatcag tcgttagcgg tttttatttt tatagcgcca gattataaat
     6001 cgccagctaa aaacagcaac agcattccag atacgccgcc aacgccaagg ctgctgaatt
     6061 agtcgccata gccactccag tccgcagcgc cgccataatt ggggcgctct tttgatacgg
     6121 ccgatgataa aatcaaagct gccgccaata ccaatcataa tgcggcaact cggccaagtt
     6181 gtttgatgtt tggctaaaaa gtattcctga taaggcgcgc ctaatgagca gaaaaccaat
     6241 cgtggttgaa aatcgtttaa tcgtgatagt tgtcgctggc ccaaataacc attgcgaccg
     6301 cagtcttcaa ttagacaacg gagtttagga aatttagtca acaaagccgg ccgtagaatc
     6361 gccgccggcg acagcccgcc acgccaatta ataatcgcta tgggccaacc attcttttca
     6421 gcctgaatta ataagtcggc cgttaagtca gcaccggtat gacggattaa tttttggccg
     6481 taagttaaag cggctagact taaaccgaag ccatctggta aagataaatc ggcttgatta
     6541 aggatattgc tgaaggtttg atttttttgg gcagccagcg taaattctgg attaactgta
     6601 acaatcagat gattttgctg gctagctaaa taaaccgcca aacgttgttg taatgcttgg
     6661 ccggttaaat tgtccaagcg gacacctaat attttaatgg ttgacataac ggatttgtaa
     6721 atatttttgt cggagtggtg ccggcaattt ttctaagcta taccttaaca taactctggg
     6781 catacggcta gcccagtggt ctaagaaatt cagtaaagct gtttgattac gcttacccac
     6841 ctctcgcaaa atccaaccag tagctttgtg gagcaaatcg tggcgttctt ttaaatattg
     6901 aggtgccatt cgtaaagccg ttcggtagtc gccgtgttta atgagagtta aattagccac
     6961 catagccgct cgcctggccc acagattatc attgcggcgc agttctttta atagtaagag
     7021 ctttttattt tgattattaa ctaaatattg accgataatc ttaggggcag ttaaatcaat
     7081 taaatcccaa ttattaaaac tagcaatatt atttagataa aaatctaaat agtcttggtg
     7141 ttggctcgga ttatgccggc attgatcaac caaaattaag ccggctgtta ggcgaacttc
     7201 atgaattgga tcagctagca tttttttaat aatatcaagt gacaaattag cgaaacgctt
     7261 ggcaaccgtt cgctgttccg gtactgtaat accccaaaag atatcgccct gaccatattc
     7321 ccctggccca gttttaaaaa aacggcccag aatcgctgct ttagctggat tagctagctt
     7381 ttgtagagct tggcgaataa ccgagtagct attttgaact gatttattcg ttttcataga
     7441 tgtggttgat aaaaaactcg ggccagatca gtgacgcaat tattcttaat aacgataccc
     7501 tcttgttgta agagctgtcg ttttttattg aggccagtag caaagccagt taactggccg
     7561 gtgctgccaa cgacgcgttg gcaaggcacg actggccaat atggattgcg attaagagct
     7621 tggccgaccg cccgagctgc ccgtggccga ccaatggctt gagcaatggc accgtaagta
     7681 cttaggcgtc cccgagggat ttgctgggtt accgagtaga ctagcgaggc caaaggagtg
     7741 attggcggaa ttattttttt cattaagata attatagcgt tttttggcta ttaagaaaat
     7801 aaaaaggcct ccctagggag gccttataaa gtgtttgtgg ttaatcagcc cgaggacggg
     7861 ctgagaagct ggtttctagc cagcttctaa ttgcggcgat ttcttccttg gttaaggaag
     7921 attcgaaatt atcagcgtag aagccgctga taatttcttt caatcgctgg aaacagtatt
     7981 ctacggtttc gtcatcttcg ttgacgcata tgtgcgccaa caaagctcgg ctaatgattc
     8041 tcctgatata actgagccgc ccagcggcaa ttatttggag tgcctgttta caggcttttt
     8101 ctttgccaga caggtagaca tttactagta atggaaccag atttaccatt actacactga
     8161 gcgttatgtt catgctcagt gtaaatgctt gtctgcccac cttgttgata aacaaggcca
     8221 atattagaca gttaattaag gacaaaaata acaaaactag ggttattttg ttaattttca
     8281 tccaaatttt tagccgactg gctatttttt tcgcttttga acttagcgcc acccgttgag
     8341 caaccagttg ctcgaagtcc tggcaggcgc tattcacaat ctgaacggcg agatttttgg
     8401 atatttccat gatctcctcc ttttattgta tttatttttt aggtacacta gtattatttc
     8461 aaactaccct taattgtcat tgttgtcaat aactgattgg ttaatttttc gctcggcgat
     8521 ttttatgcta taatcttgac agacgacgtt ggctcggtta tattattttt agcagtctag
     8581 acaagtgact gctaaataat tagcttagta gcctataaaa taatatggac atgttagaca
     8641 aatttactca aaaatcacaa gaagctatta ttaacgctca gatggttgcc cagcagttcg
     8701 gtcaggagtt tattggcgcc ctgcatctgc ttttagccct attacagcaa gatgagagtt
     8761 tggtgcgccc aatcattgaa gcgcaaaaga ttgatccaga tttagtggct agtcagacgg
     8821 aggctgagct ggacaagttg ccgaaatccg atcgggctga agttgccgaa ggcgtggttc
     8881 agggcacagc tgaagcctca atggtgattg aacgagcccg caaagaagcg aaaaatttag
     8941 acgatgaata tattgccacc gagcatttac tattagctat tgtcggcatc aaatccaagg
     9001 cgcaatcgat tttattgaaa gtcggcttgg gttatgaagg cctgcttaag gccctgattg
     9061 agttacgcgg ttcacaggcg gccggatcaa ctgatgccga aaagaagtac aggattttac
     9121 agaaatatgc tattaatttg accgatttag ctcgtcagaa aaaattggat ccggtaattg
     9181 gtcgtgatga ggaaattcgc cgtattatgc aggttttgtc acggcggacc aagaataatc
     9241 cagttctaat cggcgaagcc ggtacgggta aaacggctat tgtagaaggt ttagctcagc
     9301 gaattatcgc tggtgatgtg ccggaaacat taaaaggtaa agaattggtc agtttagatt
     9361 taggcgcttt aattgccggc gccaagtttc gtggcgaatt tgaagaccgt cttaaatcgg
     9421 ttattaaaga agtgcagaaa agtaacggtc aagttatttt atttgttgat gagcttcata
     9481 ctattgttgg ggctggcgcc tcagaggggt caatggacgc ttccaatatg cttaagccgg
     9541 cattggcccg cggtgaaatt aaattggtcg gcgccacaac cactaaagaa tatcaaaaat
     9601 atattgaaaa agacgctgcc ttggaacggc gtttccagcc aatttttgtg gaagaaccga
     9661 gtgttgagga tgctatcgct attttgcgtg gtattaaaga aaaatatgaa gttcatcacg
     9721 gtgtgcggat tactgatgat gccttgattg ctgcagtcaa actatcggct cgctatatta
     9781 ccgatcgttt tttgccggat aaagcggttg atttaattga tgaagccacc tcgagcttgc
     9841 ggatggagat tgattcctcg ccggacgaat tggacggctt aaaaagagaa attcgtcgtt
     9901 tagaaattat gaaagctggc ttattaaaag aagataataa agaaaaagtc cgtaaggtca
     9961 atcgtgattt ggaaaattta aaggaaaaag ccaaacaatt ggagctgcat tggtccaatg
    10021 agaaagaaat catctccaag attcgccaat tcaagaaaga tattgatgaa ttgcgggcac
    10081 aggcggaaat tattgaacgg cgcggtgatg atttaactaa agtcgctgag attcgttata
    10141 gccggattcc ggatttagaa aaagctattg aaatggagcg gcagaattta ctaaagattc
    10201 agagcaacgg ccagcggatt ttaaaagaag aagttgacga agaagatatt gctaaagtgg
    10261 tggcgcgttg gactggtatt cccatagctc ggatgctgga aaccgaagct cagaaattaa
    10321 ccaaaattga agattatctt aaggctcgag tggttggaca agttgaagcg attgtggcgg
    10381 tggctaatgc tattcgccgg tcacgggctg gtattagtga agaaaagaag ccgattggtt
    10441 ctttcctatt cgtcggtccg accggtgttg gtaagaccga attagccaag actttggctg
    10501 aatttatgtt tgatgatgag aatgccttga ttcgtttaga catgagtgaa ttcatggaaa
    10561 agcatagtgt ttctaagatt ttgggagcgc cagccggcta tatcggttat gacgatggca
    10621 gccaattgat tgatcgggtg cgtcgccgcc cttatagcgt aattttattt gatgaaatcg
    10681 aaaaggctca ccccgaggtt ttcaatatct tgctgcaaat tttagacgat ggtcgcctga
    10741 ctgatgctaa aggccgagtg attaatttta aaaataccat cattattatg acttccaatc
    10801 taggtaatga agtcatcaag gattattcca ttggttttta tgatggtagt gatgccaaga
    10861 aattggctga aatccgcgag gacgagatga aagacaagat tgacaatatt ctcaagcagc
    10921 attttaagct ggaattctta aatcggattg acgaaattgt tatttttaag agtttaagca
    10981 gagaagctct aggtaatatt gttgaattag aactacagaa agtcagccga cgtttagcca
    11041 ttaagggtat taaatttaag gtgacagcca aggttaaacg atttttggct gaccagggtt
    11101 atgatattac ctttggcgcg cggccgctga aacgagttat ccaaaatcaa ttattagatg
    11161 aattggcttt acaaataatt gagggcaaga ttaaagaggg agatatgatt aatgccgatt
    11221 tatcgtcagg taataaaatt atttttaaga ccggcgctga attaaaaaat catcaagatt
    11281 aaccacatga aatcattgac cagcaaagtt ttgttaatta tcggtttaat agccagtagc
    11341 tggctagtta acggcggccg ggctcaggcg gctgatttta accccaatct aattattacc
    11401 gaccaggaga ttatggatag cgcggctatg aatttgggag agattcaaaa ctttctggaa
    11461 gatcgcggca gttttttggc tacttatcga accactgatt attatggcgt tgttcgtagc
    11521 gccgctgaga ttatctataa tgccgcggtc aatagctatg attgtagcgg cattgaagtc
    11581 aataacccac aggacattga agaggctaaa attaagtgcg tcaaggtagc aatcaatccc
    11641 cgttttttaa ttgtgctgct acaaaaagaa caaagtttga ttgatagcaa aggaccacga
    11701 caaagccaat tagattgggc gaccggctat ggctgtccgg ataatgctgc ctgcggcgaa
    11761 cgctggcgtg gctttggcaa acaagttaac gcggcggcgc tacagttcta tgattacatg
    11821 gttaatcctc gttattatag atatcaagtt ggccaaactt atactattac taataccggt
    11881 cgtgatccga tgattgttgt tccggctaat cgggctaccg ccgcgcttta taattatacc
    11941 ccccacgtct ataacggaaa ctataatttt tggaatattt ggcagcgcta tttcactcag
    12001 gtatacccag atgggtcttt gctgcaagcc gccggtcagg agacagttta tttaattcaa
    12061 tccggtcgta aacgaccatt ctccagtcgc ggcgccttag tttcgcggtt taatcccaat
    12121 aagatcgtga ttgttagacc ggaagattta gacagttatc cagagggcgc gccgattaag
    12181 tttcagcaat atgctttagt ccaggcaccg tctggagcag tttatctctt ggttgacgat
    12241 aagcgccgcg gcttcaccag tcagcaggct ttcgctaaga tgggcttcaa tcgccaagaa
    12301 gtcatcaaag gcagctggga ggaattggct gcttataagg aatcaactcc gattacggtc
    12361 acctcgacct atccgaccgg tgcattattg caagacaaga aaactggcgg cgtttattgg
    12421 gtagaagaag gtaataaggc gccgctgcta gatcgggcaa ccctggcggc taaatttgct
    12481 aaacggcgaa ttattccagc tgaccaaaaa gaattagatg cttataaaac ggttgatccg
    12541 gttaatctgg ataacggtag cttaattaag ttggacagcg accctaatgt ttatttatta
    12601 gatcatggcc agcggcggct gattgctagc gagcggatat ttaaccattt aggatatagc
    12661 tgggctaata tcattactat tagttctcgt cacttcagtc tttataatca agggccaatc
    12721 attgaagata atttgcccgc tgactatgga caacccatgg tcattccaac accagacaac
    12781 ctagtgccaa cagtaaccgc cactacgacc agctcaacct caaccaaccc ttaataatat
    12841 ggctttagct tttgccggcc tagtgccgca ctcaccgctg ttggcgccaa atatcggtaa
    12901 agaacatcgg gctcagctcg ggaaaacgct agaggcctat agcttattgt ctaagaattt
    12961 ggcgacgact cagccggacg cgatagttat tatttccgct catgccgcta gcgggccaga
    13021 ctggtggaac gccaatgttt gcttgaatta tcaggttgat ttaagtttat ttggcgattt
    13081 attaacccaa ggacaatggt cgggcgcttt aggtttagct gaagaattac aaaatttttt
    13141 aggacaaacc gaacaattgc gtttttatag ccagccacaa ttagattatg gcacagccgt
    13201 gccactgctg ttattaggtg aagatttaaa aaccgtgccg attttgccga tcggttatat
    13261 agataatcag ccggagcggc aggcggaatt cggtcggcgg ttgggggaat ttttgcggca
    13321 atggccaggc aaagcggcgc taattggttc tggtgattta gctcattgtt taggagctaa
    13381 tcagccgagc ggtctgcaag ctcgtggcca taaatttgac cagcgactgc aagaagcttt
    13441 gcggcaaaat gatagccagg ctcttttgga gttatccatg gataaagcta atgaaaccgg
    13501 ccaatgtgct tggctggcct atctgacttt acaggccgct ttaaatggac aaccggggtc
    13561 gtggcagaat ttggcctatg aagcgccttt tggggtcggc ggcgcggttt ggcaatggca
    13621 gccggctagc gccgattaaa ttggtcttga cgataattgc ccagaatttt gaaaaaatat
    13681 tgccaacgtg agccttgttg gcagagttct tgagtgtcaa taacagcttg gcgaataact
    13741 gattcaggaa attgccgaca agcttgaaaa actgaattgc gtttgaagtt aggtatgcgc
    13801 agccgctcaa ttacttctag agctaattgt tgccaaggat aagcggtcgg cttgattatc
    13861 ggccgcggtc gtttgattaa ttgactgata tcggtaaatt ggcggatatc gcggggcata
    13921 gtaaatagat aagacaattt taagataaaa gatttataaa ttataacgaa gctatggatc
    13981 aaaagcaaat ttccagcggc cgagtccggc ggctcgtcat taatccagat tcccgtctgc
    14041 gccgcgtagc tcagacggtt gatttagcta tgcttcaaga taagaagttt caagaattaa
    14101 tggttgatat ggccaagacc atgttagtta aagatggtgt cggtttggcg gcgccgcaaa
    14161 tcggccgcaa ttggcgtttg attgttgtta atactgatga gggtgtctgg ccgatgatta
    14221 atccggtatt gagcgattta agttcggcga ccatagttga agaagaaggc tgcttgtcgg
    14281 tgcctaacgt ttttggacca gttcggcggt cgaaagaatt gatttgtcac tatatcaatg
    14341 atttaggaca agaagtccat ctgccggcta aaggtgcttt agctcgagta attcagcatg
    14401 aggttgatca tcttgacggc gtgctattca ttgatcattt aagtcagtaa tcatggctag
    14461 tcccaaggtt aaaatattat tagccggttc gccatcatta gctgtgccgg cttttcaagc
    14521 gatagttaat gatcagcgtt ttcaggtttt aggactatta acccaaccgg atcaaccagc
    14581 cggccgcggc ctcaaattaa ctcccccgcc aactaaggtc tgggcgctaa agaatggctt
    14641 aacggtttgg cagccggaaa aattaaaaac ttggcagtca gcgctagtca atatcaagcc
    14701 ggatatggtc gtggttatcg cttatggcca attaattcct caagcgttat tagagatacc
    14761 gccgcaaggt tgggtcaatg ttcatggttc actgctgccg aaatatcgtg gtgccggggt
    14821 attgtcggcg ccgattatta acggcgatca acaaaccggc atttccataa tgaaattggt
    14881 taaagaatta gatgccggac cagttattgc tcaagtcaaa attaatttag ccagtgatga
    14941 aacgatgggc agcttagcta gtaaaatcgg acaagtcgcc gctgatattt taccagatac
    15001 ccttataggg tatattgaaa gacgattgat tcctcaaaaa caaaatgaca gccaagctag
    15061 ctatgtcggt ttggtagcga ccgctgatgc caagattgat tggcataaat cagccttaga
    15121 aattgagaga ttggtgcgag cgatgcagcc ggtgccaggc gcttggacaa cttggcgcga
    15181 tcagcgttta aaaatcattg aggcaacagt atctgatcaa agttggctca atcccggtaa
    15241 agtcggctta attgatggtc aattagccat tggcagcggt caaggagcat tgattatcaa
    15301 tcgtttgcag ttagctggcc gtcaggctgt cagtggcaac gattttttga tcggtcaagc
    15361 tgatgttttg ggagaatatt tagcctaaag aactagacag gccaaaaagt ttttggtata
    15421 ttactagcga attataaacg ccaccatcgt ctagcggcta ggacacatgg ttctcatcca
    15481 tgcaaccggg gttcgattcc ccgtggtggt acaaaaaact gattacttat catcagttaa
    15541 tcttcaaaaa agcatatgac tgattggtca atgtttaaac aggatgaatt aaataatcca
    15601 aataatattt cgcctcaccc cttctctgca tcgtcaaatc agcaatcaag taagaaaaaa
    15661 atttggctga tattattaat agcggtaatt ttaattactg tcggcggttt ttttatctat
    15721 caagcttatg cacaatcgcc cgagcggatt attaagaaaa tgtttattaa tttatctaag
    15781 gttaaaactt ggcagtattc tggtgaaatt aaaaccgaag gcaatctagg acaatcttta
    15841 accaaccaag ccaatttatc attaatggct ccgctgaatc aggataaaaa aaatagccaa
    15901 accgttgtta aatttaatgg tttgatggat attcaaaaaa ttaatgaccc ccaaatttta
    15961 ttagccctag aggttaagac tgatgcttta acatcagcga atatttcatt tggcctagaa
    16021 acaagattta tcaaccaaat tatttatgcc aagctcagca cggtccctta tttaggtttt
    16081 tttgatttaa gctcgctaaa agatcagtgg ctcaaagtgg atgtcaagac attaaaacaa
    16141 caaatccagg ctagtaaact taaaaaagag ccggataata atcaagccaa tagccaatta
    16201 tcaccagaac agataagcaa attaataacc gccatacaac gagctaatat cttaactata
    16261 acagatagta aattgcttga tgccaaaaca tataaaacga gtaatggagg taaacattat
    16321 caatatcaat ttattcttaa tccagaaggt ataaaattgt ttctagttga attggttaaa
    16381 attgttaata ataaagaatt aacttcaatt gaagctgata agttatatga aaaaatttat
    16441 ggccagcatg aattgttggc aggtaaatta ttgattagcc agaaagattt attgccaagg
    16501 cagatttctt ttgatgtaat aactaaaaca gctgacaaaa caaaagtttt atggaaaact
    16561 agccaaacca tatcgtttag tgattttaat aagccggtta gcattgatgt tccaacaaca
    16621 actaaaacat tgatggagct tttaagtgga tttgctcaaa aattacctgc tcatcaatat
    16681 aatagtagtt ctactaccac gccaacatca ataatgccac atattaaaca ttcattaaac
    16741 gattcagata atgatggctt gagcgataat gatgagctta atatttatag tactgatttt
    16801 ttaaatccag atagtgatgg cgatggttat tcagatggtg atgaagttaa gggcggttat
    16861 aatcccaatg gttccggcaa gctattgatt gatgatctta cagttgacaa atcggcgcca
    16921 tttttgataa aataaaacta ttgataaaac gcccgccagg gttgttttta aaaacaagat
    16981 ttatggagaa aattacggct tattttcgtt cagcttgggc ggaaattaag aaggtcacct
    17041 ggccgaccaa aaaagaaacc tacaactaca ccctgctagt cattggcatc agtttgggca
    17101 ccgccatttt tttgggcgga atagatgctt tattcgcggc gggcttgaag ttgctgatta
    17161 agcaataatc aataatttta gttcattcct atggctaaac aaactcttaa tcaaggacgg
    17221 cgctggtatg tcctgcacac ttattcgggc tatgaagaaa atgttgccca gaatattaag
    17281 cagcgcattg aatcgatgga catggaagac aagattttta atgtcatgat tccgaccgaa
    17341 aagaaaatta aaatcgttaa cggtaagcgc aaagtttatg aggaaaagat ttttcctggt
    17401 tatattttag tggaaatgat tgtaaccgac gattcttggt attctattcg caatacgcca
    17461 aatgttaccg gttttgtcgg tactggcact gtgccaactc cgattgatga agccgagatc
    17521 aaagaattac aaaagagaat gggtcttgat gaaccgaagt ttaagattga tgtgtcagtc
    17581 ggctcgccgg tacggattat tgatggtccg ttcaaaaatt tggagggcaa ggtcactgag
    17641 gttgatgaaa atcgtggcaa ggttaaatta ttgatttcca tgtttggtcg cgagacccca
    17701 gtagaattag attttttaca gattaataag ctttaattac cgataggctg gtggcgggcc
    17761 gataacggca cgcttctccg ctcaccgcca cttaaacaaa tgtatggcta aaaaaatagt
    17821 tacccagata aaattacaga ttaaaggcgg tcaagctaat ccagccccgc cggtcagtcc
    17881 agccttgggc cagcacggtc tgaatattgc tgatttttgc agtaaattta atgacgccac
    17941 tcgtgataag atcggcgatg tcgtaccggt tgttattact gtctatgctg atcgcagctt
    18001 tgattttatt actaaaacgg cgccagcctc agaattaatc aagaaggcgg ctaagatcaa
    18061 caaaggttcc agtaaggcgc tcaaagaaaa ggtcggcagt ttaacgacag ctcagttgca
    18121 agaaattgcc gaaactaaga taagcgattt gaatgcccgt gacgttgagg cggcgatgaa
    18181 aattattgcc ggcaccgctc gtcaaatggg tattgagatt aaagattaat taatttatta
    18241 aataggatcg tgggagctag ttagtagtta gctagcactc gcaccacaat cgtatggcta
    18301 agaaagtaca gaaaaacaag actgccaaac aaggtaaggc acaagccgct gtccgtcaac
    18361 tggttgataa gactaaaacc tatgttttgg ctgaagcgtt ggagttagta aaaaaaacca
    18421 gcgttaccaa gtttgacgct tcggtggagg ttcatttgcg gctcggtatt gatccgcgca
    18481 aaggcgatca gcagatccgt ggagcagtta gtttaccgca tggtactggc aagaccgtca
    18541 aggtggcggc gtttgttagc ccggaaaatg aggcggctgt tagagaggct ggtgctgatt
    18601 tggtcggcgg cgatgaattg attgaagaaa tccgcaagac tgaaaagact gattttcagg
    18661 tagcggtggc cgaaccagct ttgatgcgca atttggcggt tatttctaag attttaggca
    18721 ctcgcggctt gatgccatca cccaagaatg aaaccgtgac gactgatcca gccaaggcag
    18781 ttactgaatt aaagaaaggt aaagttagtt ttaagaatga cgatactgcc aatctacatg
    18841 tcgctattgg caaggtttct tttagtgttg aacaactagc cgataatttt cagtctttaa
    18901 tagaaattgt taagaagaat aaaccgtcgg cagctaaggg tacttatatc aaaagtgtaa
    18961 ccatctgttc gtcaatgggg ccgggtatcc gagttttatt gggccaataa tcctttgctc
    19021 cgttagcgga gcgctttata aaaaaaccaa tctttttatt gattggtttt ttttgtttaa
    19081 gttatcggta aatctacttc aatggctccg ttgccgccaa attgcggttt agcggttcgc
    19141 tgacgatagc cccgagcttg gagccaagcg gcagtaaaat ctttaagtac accatggcca
    19201 tcggctgaat gccagccttg gccagtaata atcagtaatt tgacgccgcc gccttgcttg
    19261 atccagtagt caaaaaagcc aattagcgcc gcttcggctt cgcggcggct atgaccatgt
    19321 aagtccagtt tagcggctgg ttcttgcagg tatttattga ctttacgttg aagcaaacgg
    19381 cggttcataa tagttgatta tagcatcttt tcaggcttgc cgaaagctgt tgttatctga
    19441 taaaatgatt ataaagaata gttaaaatta atattttatg ccaagcagac catctaaatt
    19501 ggagtattat atgaatattg ccggcgaagt agccggtcgt tccacttgtt atcgggctaa
    19561 acacggtgcc attattgttc gcgacgatca gattatttcc actggctatc tggggtcgcc
    19621 gcgtaagacc aaagattgtt gggagcgcgg taattgtctg cgggaaatgc ttaaagtacc
    19681 acatggccat cgttatgaat tgtgtcgcag cgttcatgcc gaacagaatg ctattattaa
    19741 tgcggctcga gccggtgcta gcctgctcgg ctcaaccata tatctatatt ctacagttat
    19801 ccgtaatggt caagaaaaac cgcaagatgc ttatccttgt tttatttgta agaaaatgat
    19861 tatcaatgcc ggcattgaaa aattggtcgg ccgtcaagcc gacggcagtt ttaaggttta
    19921 tgaaattagc gattgggtta aagattggca agaaagagat atgctggacg ataacgagca
    19981 atatggtatg actgttaata tagagcaaac ttaatttgtt gatttaaata atttatgaac
    20041 aagttgatta ttggtctgac cggtcaatta gccgccggca agacgactgt tgccaaatat
    20101 ttggcggaaa aatatggcgg caaggtttat ggattttccg gtcccttgcg cgatattgta
    20161 gatcgtttga cattaccgca gactagagaa aatatggcta atctatcgtc aatcctcagg
    20221 cgtcaattcg gtcaggatat tattttaaaa gcgattatgc gcgatgtgca aaacgatcct
    20281 tgtccgttta tactcctaga gggtatgcgc cgctatggtg attttgtggc tttccgcgag
    20341 ttccctaatt ttcatttagt ggcggtcttg gctgaacaga agttgcgtca tcagcgttta
    20401 actaagcgcg gccagaatcc agatgacgct agtaaaactt tggagcaatt taaaattgat
    20461 gaacagggcg aggccgagca agatattccg cgagcgattg ccgaagccga tttccaaata
    20521 gataataatg gcgatttagc tgccctctat gaacaagcag aaaatattta ccgacaatta
    20581 gccggttaaa aattaagatt aaatttatgg ccggaaaatt aattgttctc tacggtatta
    20641 ataatttagg caagagtact caggccaatt tgctggttaa tcgtttacgc gatgctggtt
    20701 atgccgctag ttatttaaaa tatccgattt atgaactggc accgagcgga ccgttgctta
    20761 acgcctattt acggaaaggt aatccactgc agctatcgcc gcgggaagcg cagattatct
    20821 atgctttaaa tcgttttcaa tatcagccgg agctagttcg gcaattggcc gccgacgaat
    20881 ggattattgc tgaagactat accggcaccg gtctagcttg gggctggggg gctggcgtca
    20941 aacttaattt tttggaagca atcaatgaag gcttattgaa gccggatttg gctttgtggc
    21001 tggacggtca gcggtttgtt agcggtattg aacaaggaca taagcatgag aataataatc
    21061 agttaatgga taaagttcgt gaatgccatc agcagttagc tgaacgttat ggctggccgc
    21121 gttttaatgc caatcaatct atcgataatc tcaacgatca attgtggcga gcggttcaag
    21181 ctttatttta agccccaaac gattcaagat ttattatgga acaattaatg ccggtcatcg
    21241 gcctagaaat tcataccgaa ttaaagacca agagtaaaat gttttgccgc tgtgataata
    21301 ataccgaaga acaggcggct aacactttgg tttgtccgat ttgtctaggg catccaggta
    21361 ctttaccgca gcctaaccgg caagcggttc attttgggct gctcctggct aaggctttaa
    21421 attgtcaaat aaatcagcac agtcgtttcg ctcgcaagaa ttatttttac cccgatctcc
    21481 ccaaggggta tcagataact caagcagata ctccactagg gtataatggt aaattgtcaa
    21541 ttgatggtcg ggatattttt attactcgta ttcatttaga agaagacacc ggtaagttgg
    21601 ctcatgataa aaataaccag catagcctgg ttgattttaa tcgggccggc actcccctga
    21661 ttgaattagt caccgggcca gtaattacct cagctcaaga ggcgcgacgc tttggtcaag
    21721 agtttcaaaa attattacgt tatttaggga tttccgatgc caatatggaa aaggcggaga
    21781 tgcgctgtga agccaatatt agtttacaga cagccggccg ctatcagatt gacggcgcga
    21841 ctattagcgg cttggctggt taccagttaa ataacaaagt ggaaattaaa aatcttaatt
    21901 cgttcaaggc cttagagcga gccattgatt atgaaattaa gcgtcaaagc gaagtgttgt
    21961 ctagcggcgg ccaagtggcg gctgaaacta gaggctggga cgaagcggac aacgctacca
    22021 aggctcagcg agtcaaagag gcgacggctg attatcgtta ttttactgaa ccggatattc
    22081 ccccgctggt aattgacgat cactggttag caatagccgc tagtgaagta gataaaattg
    22141 aattaccttt agctaaggcg cagcgctggc aagttgatta tcaattgtca gccgataacg
    22201 ccaatttact gaccagctgg ccggccacag cgacttatgt tgaccaggtt ttagtcttag
    22261 ccacccaagc tcggccaaag gcggcggcaa ttatcactaa agtggctatt aattggctga
    22321 ctagcgaagt cgctaagcat tgccgtttag ataatctggc taacagtccg attaatccgg
    22381 aacatttagt tgatctggca atagctatta ctgataacaa gattaattcc accgccggtc
    22441 aagcggtact agccgaatta attaataatc ataaccttag cgtggcggaa gttattgaga
    22501 agttggattt aggtcaagtg gacaatagcg atgagttagc taaactagct caggtcatca
    22561 tcgccgaata cccccaacag gtatctgaat accgggccgg caagactccc ctgatgcagt
    22621 tctttgtcgg tcaattgatg cgccgcagcg gcggtaaagc taatccacag ctggcgcggg
    22681 aggtattaga gaaggaatta aatagataat gactgtctga gccagttttt aacagtgttc
    22741 ggtcgcaaat caagatcagt taagtcttgc gcgctcaacc attcaaaatc aaacctaccg
    22801 acataggcgg agtctttttg aaattctggt ccatcacctg agcccagagt tccgcttata
    22861 atattgcagg cgtaaaaata ctccatttgg ccgaaagttt ctggcttggt tgaagaaact
    22921 tcaattaaaa gctctctggg ttcaattatc aagccgagtt cttctttaat ctccctaatt
    22981 aaggcttgtt gattggtttc gccagattca acctcgccgc caggtataac ccaataggtt
    23041 tgatcgggtt tagtccgctt aatagttaaa actttgccgt gatcaataat gatagcccgg
    23101 acacgcttgg tatgcatgat ttatcgatta attgttgaaa ttatttttgc ctctatgtcc
    23161 cgtgggttat aactataagc attttttatt ctaattagcg gtaaaccggc cgagttaaag
    23221 acctgattaa taaaatcgtc tctctcaatc cgtgattgcc atttatgaga gctatcatcc
    23281 aattcaatgg cacagactgg attaatgttg cgtggctcgc aaaggacaaa atcaacatgc
    23341 ttttgaatta ttctggcacg gctggagcgg ttatagtgag gcacaaaaaa taaatctatt
    23401 agtctgactt tagaaaagat aaataaattg tggcgatcag cgatgggctt cagcgcaaag
    23461 aaaaaattct tttcggcatc agttaggaag tatttatttt tatagtaaat aattttttga
    23521 tctttcgccg gtgttagttg ccttgatttt aaatatttta agataataac cgttataaaa
    23581 gctattaata atattggtag tatttccata ctttttacta ttaaggataa acctatacga
    23641 tttggcttaa ctatttaatt agctggctat tttaaatctc cccatttttt tcagcttaat
    23701 actgttgtaa tcatcgacat cgttaaatac aaattgccaa atagaataaa tgttagtttt
    23761 atcatctttt tcaaaaaata aagttataac ttttttcttg gtatgcgttt gccactgtct
    23821 aaatggatag taaagttgcc taataataat atcttttgtg tttttgttct tggcctctat
    23881 tagaactatt tgatcccggc cttcgtagcc agcatcaact tctgtttgca cgccagaaac
    23941 attaatttgt tgtttgccaa caaagaaatt aaaatcagga gtatattttc tacccctaat
    24001 agttaatact agggttgaat cttccataaa agttctgatt aagctagagg catacgcaaa
    24061 atctaaatgc tgcatttctg aattacctat taaagatgag tcaagattaa agtctaattt
    24121 tgaagcatat ataatgactt ctgattttat ctctggaata tcaacatagc cctccccctt
    24181 aattatgttg taatgaccat ttttg
//
LOCUS       DAOPAI010000008        21487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_2588_length_21487_cov_5.537561, whole genome shotgun sequence.
ACCESSION   DAOPAI010000008 DAOPAI010000000
VERSION     DAOPAI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21487
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_21487_cov_5.537561"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKV49_01740"
     CDS             complement(<1..443)
                     /locus_tag="PKV49_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22368.1"
                     /translation="MSNGVLIGGLIISFLVLAQGADILVTNIKALAARLKVPIFFLGL
                     LLGLFTSMPELILGISSSRYNLNNVALGNLLGGIIVLFGFVLGLAIIFNRQIKTDGNL
                     ATVAPGPLLFLLPLILGLKGWLNRWDGLVMIIAYLMVAYYNYSHH"
     gene            complement(539..2728)
                     /locus_tag="PKV49_01745"
     CDS             complement(539..2728)
                     /locus_tag="PKV49_01745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22369.1"
                     /translation="MSIADIYDLAIIFPCYNEANRVGRTVVAYRDYLLNQPSLSQQKV
                     VLVFVNDGSTDHTSAVLNKFVGQPSANLTIQVVDYAVNRGKGGAIKAGVAAVQAKNYG
                     FVDADLAYGPELLPAILDKLQHSDFVLAQRQHGLTANFYQRWRNFLSSSLQRVTNYLV
                     GLPKIETQRGCKFFTAKVAREVILPTMENHFAFDVEAIARALQLKFRLSLIPVNFKHG
                     LGSTVTWRDGWRYLLAVVAIADRRHRQTDRRFWWHLAGWLGLIIIALFGWLVVYGYFF
                     ADDFTWLWYGHKVGWSLTKIWTYRMSTFFSPVLNSFYTIFYHVFGYRASIYLLTGLII
                     HWINALLAGILAWQLSRSRLVAVLVSLMVAGVGGAYEPLVWIAANLHTIATLWILLAL
                     VSYQQFLTSGRRQWLVWSYLAALLVFGTKEIAIVVGPLLLVTALASWPAVKSRLADWF
                     HRAYWLALLLVTIGYSWQQYLWQKDSIWVTAGHWHLSIDKIFKIPLVIADMLVPLLPL
                     AKIINSHQAALLIVIISLVFLVIIWHYRRLRLVWLGLAWSLISIAPTLLFITARWWDP
                     LASRYTYLPRLGIVLILAVIIQHHIVNNRRWRVISPMVLVIAGCLIAQLILMYQAIFQ
                     EYPFVYQAGRSLHQVLAQAAEHQPNKIYWRWGRPFETNHAHIIGAAATIANLEESQLI
                     FLENGASEARDNGDVLIYWDGPAREYRLIVNYACSHGFCSDSGFITK"
     gene            3023..3682
                     /locus_tag="PKV49_01750"
     CDS             3023..3682
                     /locus_tag="PKV49_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22370.1"
                     /translation="MLDEKKVKRLSEVFGLAKIEIRRLLTDNIEEIVLSFDDAINYLA
                     TAINDNDDCFDINNLKRCLQFVETTKQWAKIFPLLKKLVPKYGRELIADPIIQWTARA
                     KQELNEASNIVDKVNIYYCSPEWLQGSLRETIVQQAKEFINNSSSWPELFSLTQKLDS
                     RNPISRELVLEARSRLHAVLERTIPNINNTQELWKIRNEIPLPWDMQEQLIRRLYEIM
                     E"
     gene            complement(3753..4070)
                     /locus_tag="PKV49_01755"
     CDS             complement(3753..4070)
                     /locus_tag="PKV49_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22371.1"
                     /translation="MKKSLIVPLLLTVWLLGGCSLHNQAKAPVNNDSAIEPFEEVVPE
                     PAPEPVPMAEILDQSVPAPQPPVDDPIDSDSAVACPLDAQICSDGSVVSRVAPDCQFA
                     PCP"
     gene            complement(4095..4673)
                     /locus_tag="PKV49_01760"
     CDS             complement(4095..4673)
                     /locus_tag="PKV49_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPL22372.1"
                     /translation="MQIEYEATFINVDKDDVHQRLIATGAVLVKPEFLQRRIIFDLPP
                     GKQDPSRWLRVRDEGDRITLSLKVVDGDRIDNQQELCLIVNNMDAAAELLTAIGCRTK
                     AYQESKRELWRLNDVEITIDEWPHLDPFVEVEGPSEAVVQAASRQIGFDYSQALFCAV
                     DKLYELKYGIAKDVINYQTPRLVFDQVNPFVS"
     gene            4791..5168
                     /locus_tag="PKV49_01765"
     CDS             4791..5168
                     /locus_tag="PKV49_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22373.1"
                     /translation="MIELLDEPTRKKVLVISRHYPSYQQFATIKRECGDDCLITMDSQ
                     RFNDAAEIMRRYRQGGYDEIALVAPKDVLANLVASGLQPIHLLTIEVSEGDRQPGDFN
                     KGESLVRAIARKIRKVEVNITYY"
     gene            complement(5382..5585)
                     /locus_tag="PKV49_01770"
     CDS             complement(5382..5585)
                     /locus_tag="PKV49_01770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL22374.1"
                     /translation="MLDICCGTALQLRELALRGYRSIGLDASLKMLNYLQKEAQREGV
                     IVESVLADMNNFKLRQNYFTRRV"
     gene            complement(5734..6393)
                     /locus_tag="PKV49_01775"
     CDS             complement(5734..6393)
                     /locus_tag="PKV49_01775"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22375.1"
                     /translation="MDQRLAAMYSQLEITTPGLAVDIDETLSWTVGFWVEAMQQKFGN
                     PENLTIKEMVAKYRYTQNVPYWQSPEALEWMAAKINSNEIQEGLPLIDGSSVYLNKIK
                     QIIPIVAYITVRPVKVLEGTKNWLKKHNFPLAPVICRPNDIDHGDGHQWKATVLESLY
                     PNVLGIIDDNPKLLSFLNKNYRGKVFLYDHHDNFGLSFVIACRDWPTTYQAVKKTFIK
                     V"
     gene            complement(6517..7101)
                     /locus_tag="PKV49_01780"
     CDS             complement(6517..7101)
                     /locus_tag="PKV49_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22376.1"
                     /translation="MEKLNFLQPPNQEVIHSIEKKMDDFFDQLAPENTDNIIKISADQ
                     VKHLEAEGIKLEAFLDHICAKHGYLMHGSLTEIKDKYLKSQFGKVFASNRSAIAIMRS
                     IYSNRNVNLAYPYQYNDQHPLKLVVHTPENGRYISQDNGFIYLVKKDGFLNEPAGSWQ
                     FISQSPEVNYFIVVETKRADFNYPVEFKNDLDQE"
     gene            complement(7243..7313)
                     /locus_tag="PKV49_01785"
     tRNA            complement(7243..7313)
                     /locus_tag="PKV49_01785"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7279..7281),aa:Trp,seq:cca)
     gene            complement(7339..7494)
                     /gene="rpmG"
                     /locus_tag="PKV49_01790"
     CDS             complement(7339..7494)
                     /gene="rpmG"
                     /locus_tag="PKV49_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPL22377.1"
                     /translation="MSQDNMIKLECTVCKSINYFSHKNKKVIKDRLVMKKYCPKCNQH
                     TEHKETK"
     gene            complement(7524..7904)
                     /locus_tag="PKV49_01795"
     CDS             complement(7524..7904)
                     /locus_tag="PKV49_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPL22378.1"
                     /translation="MTELRQVYRCNICGNIIEVRQTGGGELVCCGQPMELLVPNTIDA
                     SQEKHVPVITAVDGGIKVTVGLEAHPMEATHYIKWIEYIVGATSYFQELLPGQAPEAL
                     FAGAPIGGQARCYCNIHSHWASMK"
     gene            complement(7915..8448)
                     /locus_tag="PKV49_01800"
     CDS             complement(7915..8448)
                     /locus_tag="PKV49_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPL22379.1"
                     /translation="MSKLGPKTAKNLQDALAGEAIARNKYNFFASVARKAGYVQIANI
                     FEETANNEKEHAKLWAKALGLIGDTAVNLQAAISGENYENQIMYPKMAEEAEEEGESE
                     LAEAFRRVGEAEKAHEARYRQLLINLNNDKVFKKDQPVRWKCNNCGYIHEGLEAPEEC
                     PACQHPRAYFEIFAETY"
     gene            complement(8481..8566)
                     /locus_tag="PKV49_01805"
     tRNA            complement(8481..8566)
                     /locus_tag="PKV49_01805"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8530..8532),aa:Tyr,seq:gta)
     gene            complement(8608..10377)
                     /locus_tag="PKV49_01810"
     CDS             complement(8608..10377)
                     /locus_tag="PKV49_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPL22380.1"
                     /translation="MSTIILFFTAAIWAYVGHLATFQWTGLGLTRWSYIPDLAALISL
                     VIIRQPFCRRRGWLIIEQGLLATYFYLSQIIKLRFFGWPLMPSDFSAAASFWRILGDI
                     NPWWQIALIGPLVLWLSLIIINWRWPRPQIWFRLGLASLVLITIFQYPARPLAALDKL
                     FGNSYWDQKTNYAQRGPWLYLLQESWRQQLAFQLPSRIEVEQAAAQLDYRPISTVTTG
                     SSTPNIYFIVVESLWQPDLPAVQFNRSPWADNWQQQANLGLGQALSPTFGGKTANAEF
                     ELLCGWPNVQAGMIFNGGIKQSVPCLPNYLKQLGYRTVAFHANVPAFWNRHNIYPLLG
                     FDHFYSLADYRQDDMNDEFMADSSFYQQTLDKIKAQDQSQPSFNYLLTYSNHLGYPLN
                     ANRPAIISTTPAIAAVAERYFNSLYYTTKELAEFLEQLHQIDPAAVVMVVGDHWPFLG
                     DDNIGYRQGGWWPDGQLSAQQIWQRSITPLWARQANDYWLKDRQLAFYQLPDALWQQL
                     GFGNSPFATWSASLNANWRPLDSLGEPGVIKNQEGDYQFCYPGANDDQCSIINHQHQA
                     TRTLILDIFRGRQDSQISIDEAG"
     gene            complement(10424..10496)
                     /locus_tag="PKV49_01815"
     tRNA            complement(10424..10496)
                     /locus_tag="PKV49_01815"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10461..10463),aa:Thr,seq:ggt)
     gene            complement(10503..11336)
                     /locus_tag="PKV49_01820"
     CDS             complement(10503..11336)
                     /locus_tag="PKV49_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPL22381.1"
                     /translation="MLLFWLSLILVAVALTVLFFIFFHKMPLLANIDIANLPAEREAA
                     KKQAIIDERIRRQLARHGWWWRNLLGPFYQRLKNLFWRGYGQLQKLDRQHLKARLQAQ
                     GLNWQRAKQLLADAEEALKNNDLVQAEKLAVESLELDKSQLDSFIILAEIYQEQKKYQ
                     EAEETWIYAIKRLSQKYRADQSRGRQEEARETGRRLAEYQYSLGQLYLRQDNPDRADY
                     YLKQALKIEPKNPRYLDTLLEISIIRKDRVTAVLIFGTLSEVDPSNAGLAEYQEKIRQ
                     L"
     gene            complement(11340..12083)
                     /gene="tpiA"
                     /locus_tag="PKV49_01825"
     CDS             complement(11340..12083)
                     /gene="tpiA"
                     /locus_tag="PKV49_01825"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPL22382.1"
                     /translation="MKKLIIGNWKMALGYQQSLELAENLVLSQPPRPLLAEVVVCPSP
                     VAISSVANILEGTAIFWGAQDAAEQTPGALTGETSLESLVELDCRFILLGHSERRQFF
                     NETDAIINAKLRAVIERTTIKPVVCLGEDLATRQFGQAEEKVSRQLQAVLAGIILPAD
                     RELIIAYEPIWAIGTGQTSTPEQAQAMHQFIRQELEQLGYPNNRLLYGGSVKPDNAAS
                     LLAMPDIDGLLVGGASLTADSFWPIVFAQ"
     gene            complement(12137..13021)
                     /locus_tag="PKV49_01830"
     CDS             complement(12137..13021)
                     /locus_tag="PKV49_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPL22383.1"
                     /translation="MSVQRITIILLFNLTLWLGTGWWSAQQSAAPDVPQFVWPNRWPE
                     IKVWPASSTVVWSPQQGKLISQNSQQLRPIASLSKMVTALVVLGEKPQWSASYTLKNE
                     DQRYGNIVYLYPGETVTIKDLWQASLMASDNTATQALIRAVGLSDDQYRQKVTELAGR
                     LQISDLQIKEPTGLNPDNQASAMAIAQITNEALNQPEIQQSALLDRYTFTTKSGRQQL
                     ITNTDGFVRGSAVLPSDWQIEGGKTGYIDEAGYCFAALWKNSADQQLVSVVLGAPTKE
                     GRFTITKALVDYVQQKYY"
     gene            complement(13055..13966)
                     /locus_tag="PKV49_01835"
     CDS             complement(13055..13966)
                     /locus_tag="PKV49_01835"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HPL22384.1"
                     /translation="MSAKLTNNQLPKFGFFYLLSLISLIFTAIPAGLVAFQLINKWLP
                     DPLSGYTVSYTSSSLKFGLAALLIAVPVYFWTNHQINRGLLKKELDRRADLRRWLIYF
                     ILLVTAVVMLSWLIAIFYRYLDGELTGQFLAKSAVAIIIAAVIFLFYRYDLRRDPKKA
                     SDARYLRWFAWPVIIMLVILFISGFMIAESPKQARQRRYDEKLIGNFYQIDSVLSTYY
                     QHNNRLPDSLDELLSTPYFLDRQALQASDGQKISYKPLGGAKYQLCATWQTSNLDNND
                     GVRTIGIEKWPHQSGWQCLEQTIWSDV"
     gene            complement(13980..15284)
                     /locus_tag="PKV49_01840"
     CDS             complement(13980..15284)
                     /locus_tag="PKV49_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22385.1"
                     /translation="MEKIGRYPEPFNVQEKPLTEKLETTNKQEQSDLLDQAIFAIQLF
                     QADREYQLAGQVGNLSDSFDNFKHFTDDLQTAARLAAEKKQDSSQTDIIYQGYLKEID
                     RIYQSLPAYHLDQAYNEQYFQTRKSELTAVTSFINEIISKAKQERPELFIDDRSTNER
                     AGLLDYNLLQPTIISGREHNPVLAEILKKQGVSPLDNLLQIHFQPLFKQEGTGNRLAK
                     IKDSLAELAKVIVDKYPQVQGVTGTSWLLDNEAVCRLIGFKAIGQVEYGNWNQLIDHT
                     GQINQERLSQLVKTGHLPMKNVIGYISVVDFLQKFLPSEYRGVVNLVKLDSNWAEDYN
                     NFKNNFQIERQQLLKLFIANQINSEAELRQQLDHCTTFKMILSQAGVLDDFIELLDKH
                     NYQLNQIEKNSPQETKIIAEKINNLIVLKQQKKEISYQVEIN"
     gene            complement(15289..16410)
                     /gene="alr"
                     /locus_tag="PKV49_01845"
     CDS             complement(15289..16410)
                     /gene="alr"
                     /locus_tag="PKV49_01845"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPL22386.1"
                     /translation="MEVRTWLELDRAAIANNYQLLKDRLRSDCLVMGVVKSNAYGHGL
                     VDFSQQLAQLGVNWLGVDSITEGLALRRAGINQPILILGYTLPNLLAQARQSNCSVTI
                     SSWDNLRAWAALPVDQRPAVHLKLDTGMTRQGFVEQDWSKVLAFWQQQAGHNSLEGIY
                     THLAAAKNPSFPQFTQQQLSAFNRAIQLAKQFQQTPLSHAAATAGAIVFPEAQLDMVR
                     YGIGLYGLWPALAVREFLVEQLPLRPVLSWRSLIAEIKIVSAGASIGYDGAEKMWRDG
                     RLAIVPVGYWQGFDRGLSSIGHVLVHGHRARVVGRVSMDMITIDITDIPEAQVGQMVT
                     LLGRDGQQEITADEIALLIDTSCYEVVTRLNPLMKKVLN"
     gene            complement(16416..18203)
                     /gene="aspS"
                     /locus_tag="PKV49_01850"
     CDS             complement(16416..18203)
                     /gene="aspS"
                     /locus_tag="PKV49_01850"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPL22387.1"
                     /translation="MLRTHNCGQLRLVDNGQTVTLAGWVQSCRNLGGLLFIALRDHDG
                     LTQLVIDLTSQPQIAALAQELRDEFVIKASGQVRPRPAAMINPDMVTGEIEVAVDSLE
                     ILNRSLPLPFNLSEDKVGLASESLRLQYRFLDLRRPNLQAMLAAKDQLIRQMRYYFQA
                     RNFIEIQTPILANSSPEGARDFLIPSRLFPGQCYALPQAPQQFKQLLMIGGLDRYFQI
                     APCFRDEDSRQDRHYGEFYQLDMEMSFIDSEEDIWQIMEPLMIQLTNEFSDKQIIGLV
                     DGRLPKLTWHQAMTDYGSDKPDLRFDLKIEPVSHIVANSSLAIFQTVINDGGVIHSLA
                     VPGDHKLSRKQLDELVDLAKLKGLSGLGYLIYNGDNWQGSLAKNLESAILQELVSALK
                     LEVGQTVLIAAGQWLPVCQALGAIRSQIAQWFALADHKLAAWCWVIDFPFYEVGNTVT
                     GFDFSHNPFSMPKGGLDALKNQPPLEIVAHQFDLVLNGVEISSGAIRNHQPEIMYEAF
                     RLVGYEQAEVDRRFGAMIEAFKYGAPPHGGNAPGIDRLLMVLKDYDSIRDIYAFPKDG
                     QGRDLMMGSPSPVDQHQLTDLNLQWSKNK"
     gene            complement(18197..18916)
                     /gene="recO"
                     /locus_tag="PKV49_01855"
     CDS             complement(18197..18916)
                     /gene="recO"
                     /locus_tag="PKV49_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPL22388.1"
                     /translation="MRRTTSERLLILQRRQCQEQDAIITAFSPQRGLLRLWCRGLFKP
                     QSKLAGHVEPLTIIDGLIVWGQRPLLSAAVSRQSFGQLKSQLAAVVVASQIINRYQRW
                     LTTGQVVAEAWSDLNELLTDWDRQPLSLNILTAGSQAIQWRLGNRLGYAPDLERCVNC
                     QQLFSTSVWWRPFDGGLVCSNCRPAAANLELSAVARQYWLRLLTDEWLELTKSPLSPT
                     ILEQQTGFFNEWVNFLDHYIC"
     gene            complement(18900..19295)
                     /gene="ruvX"
                     /locus_tag="PKV49_01860"
     CDS             complement(18900..19295)
                     /gene="ruvX"
                     /locus_tag="PKV49_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPL22389.1"
                     /translation="MIEPIIYLGIDWGISKIGLAIGDSQTKLALPYGRVASWSEVEKV
                     IQREKIDQLIIGWPRHLADQTIDPHPGWQYFVDQAETSGYPVHLVDERLTTVAADRLI
                     NHQKAAADQDAIAAMLILQSFFDNYAAHY"
     gene            complement(19292..20128)
                     /gene="map"
                     /locus_tag="PKV49_01865"
     CDS             complement(19292..20128)
                     /gene="map"
                     /locus_tag="PKV49_01865"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPL22390.1"
                     /translation="MIRYKNPEQIALLHEGGQKLGRITAQLAAAVRPGVTTADLEELL
                     LQLIAEAGGAPAFKGYKSSHRSRAFPTALCASINQQIVHGPAIPGRVLKSGDIISLDL
                     GMEYPLKKPGIVSDWPINPNSPHGGYFTDMAVTVPIGKVSQVVQKLINTTRQSLQLAI
                     AQALPGNTLLQIAQAIQSFVEKRGFSVVRDLVGHGVGLAVHEDPQIPHYVCNQYGEQD
                     IVLKPGMVIAIEPMISLGSPDITTGRDGFSFETIDGSWSAHFEHTIAITATGQQILTL
                     PE"
     gene            complement(20125..20796)
                     /locus_tag="PKV49_01870"
     CDS             complement(20125..20796)
                     /locus_tag="PKV49_01870"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPL22391.1"
                     /translation="MARLVLFFFGPPGSGKGTQSANLSQQLGLPAISTGNLLRQHYQS
                     IGEHNAAMNEGQLVSDDLINKLLVERLNQPDAQSGFILDGYPRNKTQFDYFYNNIINP
                     DDKICAIEISVSDQEVQHRIAGRRFCPKCGAGYHLTYKPSLQADRCDDCQTRLEQRPD
                     DSPETIQERLALYRRTMGPLVVSWWQLGELIIINGEKNIDSVYHDILSVLQERGLVPP
                     KSEIV"
     gene            complement(20896..>21487)
                     /locus_tag="PKV49_01875"
     CDS             complement(20896..>21487)
                     /locus_tag="PKV49_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HPL22392.1"
                     /translation="GQRNIPVQYARQMHGGHSYGGSTTHLPLRVNMAGVIPIIFAVSV
                     ILFPSMIAQFFVTSKVSWLAKASTWVIGLLANQWFYGISYFILVVAFTYFYTEVVFHP
                     NQVAENLQKQGGFVPGIRPGQQTSRYLSGVLHKITLAGALFLGIIAILPLIMKSITGM
                     QSLAIGGTSLLIVVSVVIETVKQIEAQMTMRQYDGY"
BASE COUNT         6747 a         4947 c         4110 g         5683 t
ORIGIN      
        1 tgatggtggc tataattata ataagcaacc attagataag cgataatcat taccaaaccg
       61 tcccagcggt taagccagcc cttaagtcct aaaattaacg gcagtaagaa taataaaggg
      121 ccgggagcga cggtggctaa attgccatca gttttaattt gtcgattaaa aataatcgcc
      181 aagcccagaa cgaagccaaa aagaacaata atgccgccga gcagattgcc taaagcgaca
      241 ttatttaagt tgtagcgtga tgaactgata cccaaaatca attccggcat actagtaaat
      301 aggccaagca ataggcccag aaaaaaaatc ggcaccttaa ggcgagcggc caacgccttg
      361 atattagtca ctaagatatc agctccttga gctaagacta aaaacgaaat gattaacccc
      421 ccgatcagca cgccgttaga cataataaaa atagtaatca gctgattaac ttaacttcag
      481 catagcaaaa aaataggttc ttgtaaaaaa ccgcccgcta ttatgattaa ttaaaatact
      541 attttgtgat aaatccagaa tcggaacaaa atccatggga acacgcataa ttaacgatta
      601 agcgatattc ccgggccgga ccatcccaat aaattaaaac atcgccgtta tctcgcgctt
      661 cactggcgcc attctccaag aaaatcaatt ggctttcttc caagttggca atagtcgcag
      721 cggctccaat aatatgtgca tgatttgttt caaagggtcg cccccagcgc caatatattt
      781 tattcggctg gtgctcggca gcttgcgcta aaacttgatg cagtgatcgt cccgcctgat
      841 aaacaaaagg atattcttga aaaatcgcct gatacattaa aattagttga gcgattaaac
      901 aacccgcaat aaccaagacc attggactaa ttacccgcca tctccgatta ttgacaatat
      961 gatgctgaat aatgactgcc aagatcaaga ctatccctaa tcgcggcaaa taagtataac
     1021 gcgaggctaa gggatcccac cagcgcgccg taataaataa taaagttgga gcgatgctga
     1081 ttaaggacca agccaaacct agccaaacta aacgtaaacg gcgatagtgc caaatgatta
     1141 ctaaaaatac caaagaaata ataacaatta atagcgccgc ctgatgacta tttattattt
     1201 tagccaaagg caataatgga accagcatgt cggcaataac cagcggaatc ttaaaaatct
     1261 tatcaattga cagatgccaa tggccggcag ttacccaaat actatctttt tgccataaat
     1321 attgctgcca actatagcca attgtcacta ataataatgc cagccaatag gcgcgatgga
     1381 accaatcagc cagccgtgac ttgaccgctg gccaagacgc taaagctgtt accaaaagca
     1441 aggggccaac aacgatggca atttctttag tgccaaaaac taaaagtgcc gccagatagg
     1501 accacactaa ccattgtcgc cggccgctag ttaaaaattg ttgataacta accagggcca
     1561 aaagaatcca cagtgtggca atggtatgta aattagccgc tatccaaact aatggctcgt
     1621 aagcaccacc gaccccagcc accattaaac taactaaaac ggccaccagc cgtgaacgac
     1681 ttaattgcca agctaaaatt ccagctaata aagcattaat ccagtgaata attaaaccgg
     1741 ttaacagata aattgaggcc cgataaccaa aaacatgata aaaaatcgta tagaaagaat
     1801 ttaaaaccgg cgaaaaaaaa gttgacattc ggtaggtcca aatcttagtt agtgaccaac
     1861 cgaccttgtg gccgtaccac agccaagtaa aatcatcagc aaaaaaataa ccataaacta
     1921 ctagccagcc gaagagcgca ataattatta atcctaacca gccagccaaa tgccaccaaa
     1981 atcgacgatc ggtttgacga tgccggcgat cagcgatggc tacgacagcc aataaatagc
     2041 gccaaccatc acgccaagta acggttgagc caaggccgtg tttaaaatta accggtatca
     2101 aagacaggcg gaactttaac tgaagagctc gcgcgatggc ttcaacatca aaagcaaaat
     2161 gattttccat ggttggcaga ataacctccc gagccacctt agctgtaaaa aacttacagc
     2221 cgcgctgagt ttctattttg ggcagaccaa ctaaatagtt agttaccctc tgtaaactac
     2281 tagataaaaa attacgccag cgctgataaa aattagctgt caaaccatgt tgccgctgcg
     2341 ctaaaacaaa atcgctatgt tggagcttat ctagtatggc cggtaataat tccggcccat
     2401 aggctaaatc agcgtcaaca aaaccataat tttttgcctg aaccgccgcc acgccagcct
     2461 taatcgctcc acccttaccg cgattaaccg cataatcaac cacttgaatc gttaaattgg
     2521 ccgacggttg accaacaaat ttatttaaga ctgcgctggt gtgatcagtg ctaccgtcat
     2581 taacaaaaac taaaaccacc ttctgttggg ataacgacgg ctggttcagc aaataatcac
     2641 gataagccac caccgttcgt ccaacgcggt tagcttcgtt atagcaagga aaaattatcg
     2701 ctaaatcgta aatatcagca attgacataa agataaaaat cgctacttaa ataataacat
     2761 attcaaacta taataaaaac aattatcgta aatgctggtt ataaattaac tgggttaaaa
     2821 ataaattatt aacattaact atcattgacg atagacctaa tttatgctga gatacggata
     2881 agtattatta aaaatgaaat aaaaaaatcc cgtcaatctc aagcgattga tcaggccggt
     2941 aaccggctgg tcaagttatt tccgtccgcg gcggtcacac cccggatatt taatcaataa
     3001 ctaacaactc aaggaggagt aaatgttaga cgaaaagaaa gtaaaaagac tcagcgaggt
     3061 ctttggtcta gcaaaaatcg aaataagacg attactcacc gataatatcg aagaaatcgt
     3121 cttgtctttt gacgatgcta ttaactactt agcaaccgct atcaacgaca acgacgattg
     3181 ttttgacatc aataatttaa aaagatgtct tcaattcgtc gaaacaacca aacaatgggc
     3241 caaaattttc ccattgttga aaaaactggt cccgaaatac ggccgcgagc ttatagccga
     3301 tcccattatt caatggacgg ctagagctaa acaagagctc aatgaagcca gcaatattgt
     3361 agacaaggtg aacatttact attgttcacc agaatggctt caaggcagtc tcagagagac
     3421 aatagtgcag caagctaaag agtttattaa taactcttca agctggccag agctattttc
     3481 tcttacccaa aaattggatt ctcgaaatcc aattagccgg gaattagttc tggaagcgag
     3541 gagcagactt catgcggtcc tagaaagaac tattcccaat attaataata cacaggaact
     3601 atggaagatt aggaatgaaa ttccgcttcc atgggacatg caagaacaat taattcggcg
     3661 actctatgaa ataatggagt aaacgccgaa ctataaaccc cgtcacttac tgacggggtt
     3721 tagtttattg ttagaaaata ataatttatt gatcaagggc aaggcgcaaa ctgacaatcg
     3781 ggcgctaccc gactcaccac tgaaccatca gaacaaattt gcgcatccaa aggacaagcc
     3841 acggctgaat ctgaatcaat tggatcatca actggcggct gaggggctgg cactgattga
     3901 tcaagtatct ccgccattgg caccggctca ggcgccggtt caggcacaac ttcttcaaac
     3961 ggctcaatgg ctgaatcatt attaactggc gctttggcct gattgtgcaa agaacagccg
     4021 cccagcagcc agactgtcaa taataaaggc acaattaatg attttttcat aatattattt
     4081 tttaacataa tttcttaact aacaaatgga ttaacttggt caaaaactaa acgtggcgtc
     4141 tgataattaa ttacgtcttt agcaatgcca tattttaatt catataactt atccaccgcg
     4201 cagaacaagg cttgcgaata atcaaaaccg atttggcgac tggctgcttg aacgacggct
     4261 tcagatggtc cttccacttc aacaaatggg tccaggtgcg gccattcatc aatagttatc
     4321 tccacatcat ttagccgcca aagctcccgc ttgctctctt ggtaggcctt ggtccgacaa
     4381 ccaatagcag tcaacaattc agcagcagca tccatattat tgactattaa acacaattct
     4441 tgttgattat caatccgatc gccatcaact acctttaggc ttaaggtgat ccgatcaccc
     4501 tcatcacgga ctcgcaacca acgactggga tcttgttttc ccggcggcaa atcaaaaata
     4561 atccgccgct gcaaaaactc tggcttgacc aacacagcgc cggttgcaat taaacgctgg
     4621 tgaacatcat ctttgtccac attaataaaa gttgcctcgt attcaatttg catatagaaa
     4681 aagtataaca aataaatcta ataattttaa gtgggcgtta ccggcatagc ttgacttttt
     4741 cataaattat ggcaagataa cggcacaaaa ctctgaaaag gaggaaaaag atgattgagt
     4801 tattagatga accgacgaga aaaaaagtcc tggtaatctc caggcactat cccagttacc
     4861 agcagttcgc cacaatcaaa agagagtgtg gcgatgactg tctaataact atggacagtc
     4921 aaagatttaa tgatgccgcc gaaattatgc ggcggtatcg acagggcgga tatgatgaaa
     4981 tcgccctggt tgcgcccaaa gatgtgctag ctaatctggt agctagcggg ttgcagccca
     5041 ttcacctgct aaccattgag gttagcgagg gtgaccgcca acctggcgac tttaacaagg
     5101 gcgagagctt ggtcagagcc atcgcccgaa aaatcagaaa agtagaggtg aatatcacct
     5161 actactaaca aaaaactcaa caacttgccc atcgctattt ggcgatgggt tatttttata
     5221 agctgattaa catatctaaa aattaacttg caaaaataat attaaactat ttctttctaa
     5281 gcaaaacgat attattagat gacacctttt tcagtaattg agtccggctt ctctcaaaaa
     5341 aaccaataaa ttcaaatttt ccatttttct caaccaaaag tttaaactct tctggtgaaa
     5401 taattttgtc ttaacttaaa attattcata tcggctaaca cgctttctac aataacgcct
     5461 tcccgttgcg cctctttctg caaatagtta agcattttaa gactagcgtc taaaccgatt
     5521 gatcgatagc ctcgtagcgc cagctctcga agctgtaaag cggtaccaca acatatatcc
     5581 aacacagtcg ttactcctat tttactaaac tttttaataa attgttccaa taaatccacc
     5641 tgctttttga catcaataaa gctaaaagca atttcataat acttagcttg attgtatatt
     5701 tgcgtttttc atgtgcgatg tgaataattt aacttatact ttaatgaatg tctttttaac
     5761 ggcttgataa gttgttggcc aatcccgaca agcaatcaca aaggaaagac caaaattatc
     5821 atgatggtcg tataaaaaaa ccttgcctcg gtaattttta tttaaaaaac taagtagctt
     5881 aggattatcg tcaattatac ccaaaacatt cggatataaa ctctctaaaa cagtcgcttt
     5941 ccactgatgg ccatcaccat gatcaatgtc attgggccga caaataaccg gcgctaaggg
     6001 aaaattatgt ttctttagcc aatttttggt cccttccaga acttttaccg gccggacagt
     6061 aatataagca acaataggaa taatttgttt tattttattt aaatagacgc tggaaccatc
     6121 aattagtggc aagccttctt gtatctcatt agaattaatt ttagcggcca tccactctag
     6181 cgcttccggg ctttgccaat aaggcacatt ttgggtatag cgatatttgg caaccatttc
     6241 tttaattgtc aaattttccg gattgccaaa tttctgttgc atcgcctcta cccaaaaacc
     6301 aaccgtccaa gataaagttt catcaatatc aacagccagt ccaggcgtgg taatctctaa
     6361 ctggctatac atagcggcta atcgctgatc catacaggaa tatcatcaag ttgatagaaa
     6421 tcggtagcca taaatttatg gttaattttt ggttatttaa tgatcaacta tttattctgc
     6481 tcaaacataa aaacgatgcc aatccagatg gtttgactat tcttggtcta aatcattttt
     6541 aaattctact ggataattaa agtcggccct tttagtttcc acaactataa aataattgac
     6601 ttcgggcgat tgactaataa attgccatga accagccggt tcatttaaaa aaccatcttt
     6661 tttaactaaa tagataaaac cattatcttg gctaatatat cggccattct ctggagtatg
     6721 gaccactaac tttaatggat gttgatcatt atattgataa ggatacgcta aattgacatt
     6781 acgattagag taaattgaac gcataatagc aatcgccgat ctattggaag caaaaacttt
     6841 gccaaattga gactttaaat atttatcctt aatctcagtt agtgaaccat gcattaaata
     6901 accatgctta gcacagatgt ggtctaagaa agcttccaac ttaatgcctt cagcctctaa
     6961 atgcttgacc tggtctgcgc taattttaat aatattatca gtattttcag gcgctaactg
     7021 atcaaaaaaa tcatccatct ttttctcaat ggagtggata acttcttgat taggcggctg
     7081 taaaaaattc aatttttcca taaatataac tgccgagcgg tttttttaat tttttactag
     7141 gttttgtagt tcatttttat attatcatta taacacgtac attctgttag taaaaacggc
     7201 tcaacaaaaa ctaaccctat tttagggtta gttgaaataa tgcacgggat gagggaatcg
     7261 aacccccgcc aaaggttttg gagacctccg tactaccact atacgaatcc cgcagcaggc
     7321 cgctagggcc gactgaaact acttagtctc tttgtgctcg gtgtgctggt tgcatttggg
     7381 gcaatacttt ttcattacca agcgatcctt aatcaccttc ttattcttgt gagaaaagta
     7441 gttgattgac ttgcaaaccg tgcactccag cttgatcata ttatcttgag acataaacgg
     7501 atgattatct gcttaactaa aatttacttc atactagccc aatggctatg aatattgcaa
     7561 tagcaacggg cttgcccgcc gatgggcgcg ccggcgaaca gggcttctgg cgcctgacca
     7621 ggcaataatt cttgaaaata gctggtagcg ccaacaatat actcaatcca cttaatataa
     7681 tgggtcgctt ccatcggatg cgcttcaagg ccgactgtca ctttaatacc gccatcaaca
     7741 gctgtaatga ccggcacatg tttttcttga ctagcatcaa tcgtattggg cactaataat
     7801 tccatcggct ggccgcaaca aactaattca ccgccgccgg tctggcgaac ttcaatgata
     7861 ttgccgcaga tattgcagcg ataaacttgg cgcaattcag tcatatcaat aatattagta
     7921 agtttctgca aagatttcaa aataagctcg cggatgctgg caagctgggc attcttctgg
     7981 cgcttctaaa ccttcatgga tataaccaca attattgcat ttccaacgaa ccggctgatc
     8041 tttcttaaag accttgtcat tatttaaatt aatcagcaac tggcgatagc gggcttcatg
     8101 ggctttttcc gcttcaccga ctctgcgaaa agcctcagcc agctcactct cgccttcttc
     8161 ttccgcttct tctgccattt tcggatacat aatctgattt tcataattct caccgctaat
     8221 cgccgcctgc agattgaccg cagtatcgcc aattaaaccc agcgctttgg cccataattt
     8281 agcatgctct ttttcattat tggccgtttc ttcaaaaata ttggcaattt ggacataacc
     8341 ggccttgcga gcgactgagg cgaaaaaatt atatttattg cgggctattg cttcgccggc
     8401 aagagcatct tggagatttt ttgcggtttt gggtcctaat tttgacataa aaataaactt
     8461 aataataaat gagaattaaa tggtgggccg ggtaggattc gaacctacga agacataagc
     8521 cagcagattt acagtctgcc ccagttgacc gctttggtac cgacccaaaa aattaggcgt
     8581 taacgccgta tccctaaatt agcataatca accagcctcg tcaatgctaa tttgactatc
     8641 ttgtcgaccg cgaaaaatat ctaaaattaa ggttcgagtc gcctgatgtt gatgattaat
     8701 tattgagcat tgatcatcgt tagccccagg ataacaaaac tgataatctc cttcttgatt
     8761 tttaattact ccgggttcgc ccagtgaatc taatggccgc cagttggcgt tcaaactagc
     8821 cgaccaggtg gcaaatgggc tattaccaaa acccagctgt tgccaaagcg catcaggcaa
     8881 ttgataaaaa gctaactgtc gatcttttag ccaataatca ttagcctggc gagcccatag
     8941 cggcgttatg gatcgctgcc aaatctgctg ggcgctcaac tgaccatccg gccaccaacc
     9001 accttgacga tagccaatat tatcatcacc caaaaatggc caatgatcgc caacaaccat
     9061 tacaacggcg gccgggtcaa tctggtggag ttgttctaaa aactccgcca attccttggt
     9121 agtataatag agggaattaa aatagcgttc agccacagcg gcaatggctg gggtagtact
     9181 aataatcgcc ggccggttgg cattaagcgg ataacctaaa tgattggaat aagtcaataa
     9241 ataattaaag ctgggctggc tttgatcttg agccttaatc ttatctaaag tttgttgata
     9301 aaaactacta tcagccataa attcgtcatt catatcgtcc tgacgataat cagctagact
     9361 ataaaaatga tcaaagccga gcaatgggta aatattgtgt cggttccaaa aagccggtac
     9421 attggcgtga aaggccacgg tccggtagcc taattgcttt aaataattag gcagacatgg
     9481 caccgattgt ttaataccgc cattaaaaat catacccgcc tgaacattgg gccaaccgca
     9541 gagtagctca aattcggcgt tagctgtctt gccgccaaaa gttggactaa gagcctgacc
     9601 taaacctaaa ttggcttgct gttgccagtt atcggcccag ggtgaacgat taaattgcac
     9661 ggctggcaaa tctggctgcc acaaagattc gacaacaata aaataaatat tgggagtaga
     9721 actgccggtg gttactgtag aaattggccg ataatccagt tgggccgccg cctgttccac
     9781 ttcaatccga ctcggcagct gaaaggctaa ttgttgtcgc caactctctt gtaataaata
     9841 caaccaaggg ccgcgttggg cataattagt tttctgatcc caataggaat taccaaacag
     9901 cttatctagg gcggctaacg gtcgggctgg atattgaaaa atagtaatca ataccaagct
     9961 agccaaacct aagcgaaacc agatctgcgg ccgcggccag cgccaattga taataattaa
    10021 acttagccac aaaaccagtg gcccaattaa cgcgatctgc caccaaggat taatatcgcc
    10081 taaaattcgc caaaaactag cagccgccga aaaatcactg ggcattaatg gccaaccaaa
    10141 aaagcgcaat ttaataatct gactaagata aaaataagta gctagcaagc cttgctcaat
    10201 aatcaaccag ccacggcggc gacaaaacgg ctgccgaatg ataactagcg atattaaagc
    10261 ggctaagtcc ggaatataag accagcgagt caatccgagt ccggtccact gaaaggtagc
    10321 taagtggccg acataagccc aaatagcggc agtaaaaaat aagataatgg ttgacacagg
    10381 cgctaatttt taaaaacaag ctgtgacagc ttgtcttaag gggagccgtt gtcgggactt
    10441 gaacccgaga ccccttcctt accatggaag tgctctacca actgagctac aacggcgcga
    10501 tattataatt gacggatttt ttcttgatac tcggccagac cggcattgct agggtcgacc
    10561 tcagataaag taccaaaaat taataccgca gtaacgcgat cttttctaat tatactaatt
    10621 tccaacagtg tgtcaagata acgcggattc ttcggttcaa tctttaaagc ctgctttaaa
    10681 tagtagtcgg cgcgatcagg attatcttgg cgtagatata gctgccctag actatattga
    10741 tattcagcca gccgccggcc ggtttcccga gcttcctctt gccggccgcg cgattgatca
    10801 gcccgatact tctgactcaa gcgcttgata gcataaatcc aagtttcttc cgcttcttgg
    10861 tattttttct gttcttgata aatctcagct aaaataataa aactatcaag ttggctttta
    10921 tctagctcaa gcgactcaac cgccaatttc tcagcttgaa ctaaatcatt attttttaaa
    10981 gcctcttcag cgtcggctaa taactgtttg gcccgctgcc aattcaaacc ctgagcttgc
    11041 agacgagctt tgaggtgttg acgatctagt ttttgcaatt ggccgtaacc gcgccaaaaa
    11101 agatttttta agcgctgata aaatggtcct agtaaattac gccaccacca gccatgtcgg
    11161 gccaattggc gacgaatccg ttcatcaata atcgcctgtt ttttggcggc ttcgcgttcg
    11221 gccggtaaat tggcaatatc aatattagct agcagcggca tcttatggaa aaaaataaaa
    11281 aacaaaactg ttaaagcaac cgctactaaa ataagactaa gccaaaataa taacataaat
    11341 tattgagcaa aaacgatggg ccaaaaactg tcagcggtca aactggcgcc accaaccagc
    11401 aggccgtcaa tatccggcat cgctagcaaa ctggcggcat tatccggttt aacgctgccg
    11461 ccataaagca aacggttatt gggatagccc aattgctcta attcttgacg aataaattga
    11521 tgcatggctt gcgcctgttc gggagtgcta gtctggccgg tgccaatagc ccaaatcggt
    11581 tcataagcga taattaattc gcgatcagcc ggtagaataa ttccagccaa aacagcctgc
    11641 aactgccgac taactttctc ttcggcctga ccaaattggc gagtagctaa atcttcaccc
    11701 agacaaacca ctggcttgat cgttgttcgt tcaataacgg ctcgcagctt cgcattaata
    11761 atagcatcag tttcattaaa aaactggcgc cgctcgctgt gacccagcaa aataaaacga
    11821 caatccagct caaccaaact ctccaaactg gtttcaccag tcaaagcgcc gggtgtttgc
    11881 tcagcggcat cttgagcgcc ccaaaaaatg gccgtgccct ctaaaatatt cgcaaccgac
    11941 gagatagcta ccgggctggg acaaactacg acctcagcca ataacggccg cggcggctgg
    12001 ctcagcacta aattttcagc taactccagg ctttgttgat agcccagggc cattttccaa
    12061 ttaccgataa ttaatttttt catattgaat atctattttt aatattttat cacagttcaa
    12121 caatttgacc agctagctaa taatattttt gttgaacata atccactagc gccttggtaa
    12181 tggtgaatct cccctctttg gtcggtgccc ccaatacaac actaaccaat tgctgatcag
    12241 cagaattctt ccacaaagct gcaaaacaat aaccggcctc atcaatatag ccggttttac
    12301 cgccctcaat ctgccaatct gacggcaaaa cggctgaacc gcgaacaaaa ccatcagtat
    12361 tagtaatcaa ctgctgtcgg ccggacttgg tggtgaatgt atagcgatca agcaaagctg
    12421 actgttgaat ctccggttga ttgagagctt cattggtaat ctgagcaata gccatagcgc
    12481 tagcttgatt atcgggattt aagccggttg gttctttaat ctgcaaatca ctaatttgca
    12541 gccgtccagc cagttcggtc actttctgcc gatattgatc atcactcaaa ccgacagccc
    12601 gaatcaaagc ctgagtagcg gtattatcgg aagccattaa actagcttgc cataaatctt
    12661 taatggttac agtttcgcca ggatagaggt aaacgatatt gccataacgc tgatcttcat
    12721 tctttaaagt atacgaagct gaccactgcg gtttttcgcc caacaccacc aaagctgtta
    12781 ccatcttgct taagctggcg attggtctta attgctgact attctgactg attaatttcc
    12841 cctgttgcgg cgaccagaca accgtgctgc tggccggcca caccttaatt tctggccaac
    12901 gatttggcca aacaaactga ggcacatccg gagcggctga ttgttgcgct gaccaccaac
    12961 cagtccctaa ccacaaagtt aggttaaata acaaaataat ggtgatgcgc tgaaccgaca
    13021 tgcttgactg caacgaacaa acgtcaatag tttcttaaac atcactccag atagtttgtt
    13081 ccaagcactg ccaaccgctc tggtgcggcc atttttcaat accaatagtt cggacaccat
    13141 cgttattgtc caaattactg gtctgccagg tggcgcatag ctgatacttg gcgccaccga
    13201 gcggcttata gctaatcttt tgaccatcac tggcttgcaa agcttgacga tctaaaaaat
    13261 atggcgtcga caataattca tccaaactat cgggcaaacg attattatgc tgataataag
    13321 tacttaaaac gctatcaatt tgataaaaat tgccgattag cttttcatca tagcgacgct
    13381 gacgcgcctg ttttgggctt tcggcaatca taaaaccgct gataaataaa ataaccaaca
    13441 taataataac cggccaggca aaccagcgca gatagcgagc atcactggct tttttagggt
    13501 cgcgacgtaa atcatagcga tagaacaaaa atatgacggc ggcgataata atagccacag
    13561 ctgacttagc caaaaattgg ccggttaatt caccatctaa ataacgataa aaaatagcaa
    13621 tcaaccagct caacatgacc acggctgtta ccaataaaat aaaataaatc aaccaccgcc
    13681 gcaaatcagc tcgacgatct aattcttttt ttaaaagacc gcgattgatt tgatgattag
    13741 tccaaaaata aaccggtacg gcaattaata gcgctgctaa accaaatttt aagctgctgg
    13801 aagtatagct gactgtatag ccgctcagcg gatcgggcag ccatttgtta attagctgaa
    13861 aagcgactaa tccggccgga atagcggtaa agattagaga aattagcgat aataaataaa
    13921 aaaaaccaaa cttcggcaat tgattattag ttaacttggc tgacatattt ttaaactagt
    13981 taattaattt ccacttgata actaatttct tttttctgtt gctttaaaac aattaaatta
    14041 ttaatttttt ccgctattat tttagtctcc tgcggactat ttttttctat ttggttaagt
    14101 tgataattat gcttatccaa taattcaata aaatcatcta aaacaccagc ctgactcaaa
    14161 atcattttaa aagtggtgca atgatcaagc tgttgtctta attcggcctc actattaatt
    14221 tggttagcga taaataattt taataattgc tgcctctcaa tttgaaaatt atttttaaaa
    14281 ttattataat cttcggccca attggagtct agttttacca gattaactac cccgcggtac
    14341 tctgatggta aaaatttctg caaaaaatcc actacgctaa tataaccgat tacattcttc
    14401 attggcaaat ggccagtctt aaccagctga gacagccgct cctgattgat ttgcccagtg
    14461 tgatcaatta attgattcca attgccatac tcaacttgac cgatggcttt aaagccaatt
    14521 aatcgacaaa ctgcttcatt atctaaaagc caagaagtgc cagtgacacc ttgaacttga
    14581 ggatatttat caacaattac tttagctaat tcagccaaag aatcttttat tttagccaag
    14641 cgattgccag tcccttcttg tttaaacaga ggttgaaaat gaatctgtaa caaattatct
    14701 aacgggctga ctccttgctt ttttaaaatt tcagccaata caggattatg ctcacgacca
    14761 ctgatgattg ttggttgtaa taaattataa tctaaaaggc cggctcgttc attggtgctt
    14821 cgatcatcaa taaaaagttc ggggcgctct tgcttggctt tactgataat ctcattaata
    14881 aaactagtaa cggctgttaa ttccgatttt ctggtttgaa aatattgttc attataagcc
    14941 tgatcaaggt gatacgctgg taaactttga taaatcctgt caatttcctt caaatatcct
    15001 tgataaataa tatccgtctg acttgagtct tgtttttttt cagctgccaa acgagcagcc
    15061 gtttgcagat cgtcagtaaa atgcttaaaa ttatcaaaac tatcagacag attgccgacc
    15121 tggcccgcta actgatattc acgatcagct tgaaaaagct gtattgcaaa aatagcttga
    15181 tctaataaat cagattgctc ctgcttatta gtggtctcta atttttccgt cagtggtttt
    15241 tcttggacat taaatggctc tggataacga ccaatcttct ccataaaatt aatttaaaac
    15301 ttttttcatt agcggattta atctggtaac tacttcataa cagctagtat caatcaacaa
    15361 agcaatctcg tcggccgtaa tttcttgctg gccatcacgg cccaataaag taaccatttg
    15421 gccgacttgt gcttctggaa tatcggtaat atcaatagta atcatatcca tagaaacacg
    15481 gccgaccact ctggctcggt gaccatgaac caaaacatgg ccaatactgg atagtccgcg
    15541 atcaaaaccc tgccaataac caaccggcac aatggctagc cggccatcac gccacatttt
    15601 ttcagcacca tcatagccga tactggcgcc ggctgatact atttttattt ccgcaatcaa
    15661 gctgcgccaa ctcagaactg gccgcaacgg caactgttca accaaaaatt cgcgaacggc
    15721 taaagctggc cacaaaccat aaagaccaat accataacgg accatgtcca attgggcttc
    15781 cggaaaaact atggcgccgg ccgtagcagc ggcatggctt aaaggcgttt gctgaaattg
    15841 cttagctagc tggatagcac gattaaaggc gctgagctgt tgttgagtga attgcggaaa
    15901 agatgggttc ttggcagctg ccaaatgagt atatattccc tctaaagaat tatgaccggc
    15961 ttgctgttgc cagaaggcca aaaccttgga ccaatcctgc tccacaaaac cctgacgagt
    16021 catgccagta tctagcttca agtgaaccgc tggccgctga tcaaccggca gcgccgccca
    16081 ggcgcgcaga ttatcccagc tagaaatcgt cacgctacaa ttactttgac gcgcttgggc
    16141 taataaatta ggcaaagtat agcccaaaat taaaatcggt tgattaatac ccgctcgccg
    16201 taaagccagc ccttcagtaa tagagtcaac acctagccaa ttaacaccta gttgggctaa
    16261 ttgctgtgaa aaatctacca gaccatggcc gtaagcatta gatttaacca cacccatcac
    16321 taaacagtct gatcgcaaac gatctttgag aagctgataa ttattggcga tggccgcccg
    16381 atctaattct aaccaagtcc gcacttccat aaaaattatt tattttttga ccattgtaaa
    16441 tttaggtcag ttaattgatg ctgatcaacc ggactggggc tacccatcat caagtcgcgg
    16501 ccttggccgt ctttcggaaa agcataaata tcccgaattg aatcataatc cttgagcacc
    16561 attaaaagac gatcgatgcc gggagcattg ccaccgtgag gcggcgcgcc atatttaaaa
    16621 gcttcaatca tcgctccaaa acggcggtca acttcagcct gttcatagcc aactagacgg
    16681 aaagcttcat acataatttc cggctgatga ttgcgaatgg ccccgctgga tatttctacg
    16741 ccatttaaaa ctaagtcaaa ttgatgggca acaatttcta gcggcggctg attttttaaa
    16801 gcatcaagac cgcccttggg catagaaaat ggattatggg aaaaatcgaa accagtaaca
    16861 gtgttaccga cctcataaaa tggaaaatca atcacccagc accaagcggc cagcttgtgg
    16921 tcagccaaag caaaccattg agctatttgg ctacggatag cccctaaggc ttgacaaacc
    16981 ggcagccatt gaccggcagc tattaaaacg gtctgaccaa cttccaactt caaagcgctg
    17041 actaattcct gcaagatagc cgattctaaa tttttagcta gactcccttg ccaattatcc
    17101 ccattataga ttaaataacc taagccgctc aaccctttaa gcttagctaa atcaaccaat
    17161 tcatccagtt gtttgcgact taatttatgg tcgcctggta ccgctaagct atggataact
    17221 ccaccatcat taataactgt ctgaaaaata gccaagctgc tattagccac tatatggctc
    17281 accggctcaa tcttgagatc aaagcgcaaa tccggtttgt ctgagccata atcagtcatc
    17341 gcttggtgcc aagtcaattt tggcaaacga ccatcaacca ggccaataat ttgtttatcg
    17401 ctaaattcat tagttaattg aatcattaat ggctccataa tctgccaaat atcttcttcg
    17461 ctatcaataa aactcatttc catatccagc tggtagaact cgccataatg acggtcctga
    17521 cgactatctt catcgcgaaa gcaaggagca atctgaaaat aacgatctaa gccgccaatc
    17581 atcaacagct gcttaaattg ctgcggggct tgcggtaaag cataacattg acccggaaat
    17641 aaacgcgaag gaatcaaaaa gtcgcgggcg ccttctggtg acgaattggc taaaatcggt
    17701 gtctgaattt caataaaatt acgggcttga aaataataac gcatctgacg aattaactga
    17761 tctttggctg ctagcatggc ctgtaaattg ggtcggcgta aatctaaaaa acgatactgt
    17821 aaacgtaaac tttcactggc taagccaact ttatcctcgc ttaaattaaa aggtaatggt
    17881 aaggaccgat taagaatttc caaactatca accgctactt caatttcacc ggtaaccatg
    17941 tcagggttaa tcatggcggc cggccgtgga cgcacttgac cgctagcctt aataacaaat
    18001 tcatcacgca attcctgggc taaagcagca atttgcggct ggctagttaa atcaatcact
    18061 aattgggtca aaccgtcatg atcacgtaaa gcaataaata ataagccgcc taaattacga
    18121 caggattgca cccagccggc taaagtaacc gtctggccgt tatcaactaa acgcaattgg
    18181 ccacaattat gtgttcttaa catatatagt gatctaaaaa attaacccac tcattaaaaa
    18241 agccggtttg ctgctctaaa attgtaggcg ataggggcga tttcgtcaat tcaagccact
    18301 cgtcagttaa aagtcttaac caatattgcc gggccacggc tgataattct aaattggcgg
    18361 cggctggccg gcaatttgag caaactagtc cgccatcaaa tggtcgccac cagactgagg
    18421 ttgaaaatag ctgttgacaa ttaacacacc gctctaaatc aggggcatag ccaagccgat
    18481 tgcccaaccg ccactgaata gcctgcgaac cagcagttaa aatatttaaa ctcagcggct
    18541 gtcgatccca atcagttaaa agctcgttca aatctgacca agcctcagcg acgacttgac
    18601 cggttgtcaa ccaacgctga tagcggttaa taatctgact ggccaccaca accgccgcta
    18661 actgactctt tagctggccg aaggattgac ggctgacggc cgcactcaat aacggccgct
    18721 gtccccaaac aatcaacccg tcaatgatgg ttaggggctc cacatgaccg gctaacttgg
    18781 attgaggttt gaataaacca cggcaccaaa gccttagtaa tccacgctgc ggactaaaag
    18841 cggtaataat ggcatcttgt tcctgacatt ggcgacgctg taaaatcaac aagcgctcgc
    18901 tagtagtgcg ccgcataatt atcaaaaaaa ctttgcaaaa tcaacatggc ggcaatagca
    18961 tcttgatcgg cggccgcttt ttgatgatta atcagacgat cagcggcaac cgtagtcagc
    19021 cgttcatcga ctaaatgaac aggataaccg ctggtttcgg cttgatcaac aaaatattgc
    19081 cagccaggat gaggatcaat ggtctgatcg gctaaatggc gcggccaacc gatgattaat
    19141 tgatcaattt tttcacgctg aataactttt tccacctctg accagctagc cactcgacca
    19201 tagggcaaag ccagtttggt ttgactatca ccaatggcta ggccaatttt actaatcccc
    19261 caatcaatac ctaaataaat aatcggttca atcattcggg tagcgttaaa atctgctgac
    19321 cagtcgcagt aatagcaata gtgtgctcaa aatgagcact ccagctgccg tcaatcgttt
    19381 caaatgaaaa accatcgcga ccggtggtaa tatctgggct gcctaaacta atcatcggct
    19441 caatggcgat aaccatgcct ggttttaaaa caatgtcttg ttcgccatat tgattacaga
    19501 cataatgagg aatctgcggg tcttcatgaa cagctaaacc aacaccatga ccgactaaat
    19561 cacgcaccac gctaaaaccc cgtttttcca caaatgactg aatagcctga gcgatctgta
    19621 ataaagtatt accaggcaaa gcctgagcaa tggctagttg taatgattgt ctagtagtat
    19681 taatcaattt ctgaaccact tggctaacct tgccaatcgg cactgtcacg gccatatcag
    19741 taaaataacc gccatgaggc gagttaggat tgatcggcca atctgagact attcccggtt
    19801 tcttgagcgg atattccatg cctaaatcta ggctaataat atcaccagat tttaaaactc
    19861 gaccggggat ggccggtcca tgaacaatct gttgattaat agaggcgcat aaagcggtgg
    19921 gaaaagctcg gctgcgatgg ctagacttat aacccttaaa agctggcgca ccaccagctt
    19981 cggcaatcaa ctgcaacaat aattcttcta aatcggctgt ggtcacgccc ggacgaaccg
    20041 cagcagctaa ttgagcagta attcggccta atttttgacc gccttcatgc aataaagcaa
    20101 tttgttcagg atttttataa cgaatcatac aatctctgac tttggcggca ccaagcctcg
    20161 ttcttgtaaa actgataaaa tatcatgata aacactgtca atattttttt cgccattaat
    20221 aataatcagt tcgcctaatt gccaccacga aaccactaac ggtcccatag tgcgccgata
    20281 aagcgccaac cgttcttgga tagtttctgg actatcatca gggcgttgtt ctaatcttgt
    20341 ttgacaatca tcacaacggt cagcttgaag acttggcttg taagttaaat ggtaaccagc
    20401 gccgcacttg gggcaaaaac gtcgaccagc tatacgatgc tgcacttcct gatcgctaac
    20461 actaatctca atagcgcaaa ttttatcgtc ggggttaatg atattattat aaaaataatc
    20521 aaattgagtc ttattacgag gatagccatc taaaataaac ccgctttgtg catcgggctg
    20581 atttaagcgc tcaaccagca acttattaat cagatcatcg ctcactaatt gcccttcatt
    20641 catggcagca ttatgctcac ctattgattg ataatgctgg cgcaaaagat tgccggtcga
    20701 aattgctggc aaaccgagct gttgcgataa attagctgat tgcgttcctt tgcccgaacc
    20761 cggcgggccg aaaaaaaaca aaactaatct ggccatagcc aaaaaattat taatttaact
    20821 cttttactat accacaatcc attaaaacaa aacaggctcg ctagcgagcc tgcctatcaa
    20881 taatctagct aaagtctaat atccatcgta ttgccgcata gtcatctgtg cttcaatctg
    20941 tttaacagtt tcaataacca ccgaaacgac aatcaaaaga gaggtgccgc caatagctag
    21001 cgactgcata ccggtaattg atttcataat taacggtaaa atcgcaataa tacccaaaaa
    21061 taaagcgcca gctagcgtaa tcttatgaag cacgccagat aaataacgac tggtctgttg
    21121 accgggacga ataccgggca caaaaccacc ctgtttttgt aaattttctg ctacttggtt
    21181 aggatggaaa accacttcag tataaaaata agtaaaagca accactaaaa taaagtagct
    21241 aataccatag aaccactgat tggccaacaa accgataacc caggttgaag ctttagctaa
    21301 ccaagacact ttgctagtca caaagaattg agcaatcatt gacggaaaaa gaataactga
    21361 cacagcaaaa ataatcggaa tgactccggc catattaact cggagaggca aatgagtggt
    21421 gctgccgcca tagctatggc cgccatgcat ttgacgggca tattgcaccg gaatattgcg
    21481 ctggccc
//
LOCUS       DAOPAI010000009        21292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_2609_length_21292_cov_4.826482, whole genome shotgun sequence.
ACCESSION   DAOPAI010000009 DAOPAI010000000
VERSION     DAOPAI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21292
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2609_length_21292_cov_4.826482"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKV49_01880"
     CDS             <1..660
                     /locus_tag="PKV49_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPL22393.1"
                     /translation="HHTNEIAQSEAATGQPFFRYWLHNAFLNIVGGKKMAKSADNFLT
                     LAKVVADLKISPLAYRYASLQVHYRKPMEYSPDSLVAANQALYGLYRQVADLGSAIGQ
                     VASFFQAQFQQAINNDLNAAQALAVTFAVINSQLTSADKLATILNFDQVLGFNLAKSR
                     QILAVDAAAIPQNILMMFEQRQIARQAGDFSLADNLRQQLLAAGYKIEDSPDGSQLRP
                     V"
     gene            666..1604
                     /locus_tag="PKV49_01885"
     CDS             666..1604
                     /locus_tag="PKV49_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPL22394.1"
                     /translation="MVNFWSQLSKPILVLAPLAGVTDSAFRQICKSYGAQVVVSEMAS
                     VAALVHDQRKTLEMLAFQPIERPYIVQLFGSDPQQFAVAAQIITEKIQPDGLDINFGC
                     PVPKIIKQGAGAALGADLSRAREVVRAVLDNTDRPLSIKLRAFNQGISAQQFVANLAD
                     LPIAAVAVHGRSLAAGFSGFVDPTAIAACRSYVNGQLLANGGIIDLFSAQAMLAASQA
                     DGLMIGQGALSRPWIFQEIAQQVNINLSWSELSQLMLRHAKLVVENKGQAGLMELRKF
                     LAWYVKGWPQAKKIRQQLVAIKSIADLETIISNFCV"
     gene            complement(1582..2553)
                     /locus_tag="PKV49_01890"
     CDS             complement(1582..2553)
                     /locus_tag="PKV49_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPL22395.1"
                     /translation="MGKYLQVSKKIEELIIDNPKTAGSLSWLNVNSAGKPELEYLLKR
                     QHFTITHLKASMAKSLAERAFIEKETNYIFIILHFPIIKDGLVLPAEVEFFMGHGYLV
                     TIHNGDLPPLMDFFNICKKDENSLLAYELESSSVLLYEILDRLIKYCYEVLDSHSKRI
                     THAQNTIFSLDSKQRDTASEILTLRHNIIKIRKILQNHKNILQSLTEMKSSLVPAAEL
                     KKYYSTLVEHSKRIWENLENQREMIEVLNDTNESLLNDRMNRIMKTLTMMTVILYPLN
                     LIAAIFGMNTILAMPFIGDPLGFWKILFIMFLSTVTIFLIFKRKNWI"
     gene            2677..3141
                     /locus_tag="PKV49_01895"
     CDS             2677..3141
                     /locus_tag="PKV49_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22396.1"
                     /translation="MPQEQLKPSFEQMPLNNSQERQPTAEKITESAISEAAPVTVEPV
                     VNSAAQQPPAITASQPTKLTVRQEAIDDILEDGLADIYQNLPPDKQQELKQAGERTVH
                     QIDRLLDHAKSQLTKIISLIRRFLAIIPGVNRFFLEQEVKIKTDKIMALKDK"
     gene            3147..5645
                     /locus_tag="PKV49_01900"
     CDS             3147..5645
                     /locus_tag="PKV49_01900"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HPL22397.1"
                     /translation="MNWLFDFFNDSNDLLVVFLTIFITVIAVMGLIILWRYLVWRLFS
                     RHRYFDQVVYLVKLPKDRQRDQNQNFTVQQMREEIACAETIFASIGGLRAQRGFGVWL
                     WGRDDHFSFEIVANQGLIAFYVVSPRRLARYLEQQINAHYPDAMLEEVVDYNLFKPQS
                     YVAVGSLKTTRHYALPIKTYDQQEADLMNSQLNVMSKLSTDDSLAIQYTVRSAYGVWH
                     GTVKKIVELSHKKKSVSAALKHGSFDSWSDFIKENFGSKKNQEQANAKQLDKQLTAME
                     GDMLKLMEEKNAKAGLDVNVRLIACSNSQAHSDNLLNDLANALHQYNYYEYGNSFSNK
                     PRRRPAKQLIEQFIYRRFDDKASFLLNTEELSSLFHFPLQNAETPNILWLTAKQAAAS
                     ADVPNQGLLLGYNIFRGIKKDICIKEDDRLRHTYIIGKSGVGKSVLLANMAIQDVLNG
                     AGVCVIDPHGDLVDDVLARVPPERAEDVILFAPADLERPMGLNLLEFDAKYPEQKTFV
                     INEMIGIFDKLYDLKSTGGPIFEQYMRNAMLLVMADPESGSTLMEIPRVLADAEFRNL
                     KLSRCADQTVVDFWRKEAEKAGGDAALANVVPYITSKLTSFISNDMMRPIIAQQTSAF
                     NFRDIMDKRKILLVSLPKGLVGELNAYLLGMILVGKLLMAALSRADMPKEERRNFFLY
                     IDEFQNFTTNSVCQILSEARKYGLGLIIAHQYIGQLVARGDTAIKDAVFGNVGTIISF
                     KIGSEDAEVLQKEFAPTFDAYDLINVEKYTAYIKLLINNTASRPFTLKTPWPLAGQES
                     LDLAGKIRQLSRLKYGQDRSLVEAEISQRGKFNL"
     gene            5649..6302
                     /locus_tag="PKV49_01905"
     CDS             5649..6302
                     /locus_tag="PKV49_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22398.1"
                     /translation="MSSSTDKNQDPVNNDVWQPDQAIVFNQPDQPQPTSSHQSPVKDS
                     QPLDQSGALQNIASRLELLAQELRSIIQTTSQPSLADHQVIDSLTQPIDDGDKIIEGY
                     FDGEQMIATTGQAYAVPANYASKSKLVAGDSLKLTIGPRGHFLYKQVSPVERRRLVAS
                     LEQAPDGNYYAVYEHQRWRLLKASVSYFRAQPGDRIAILIPQDLPANFAALENLVAE"
     gene            6308..7195
                     /locus_tag="PKV49_01910"
     CDS             6308..7195
                     /locus_tag="PKV49_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPL22399.1"
                     /translation="MLGWWPDKKIINSFKQLTAADEELLKKAWNLARHLHAGQKRKSG
                     QPYFTHPVAVAEILAEKFADPVLTAAALLHDTVEDCPDCLMGDIYRDFGTQVGFLVDS
                     VTKDRSNFYCQGQEFDDDIERLLWAGGQDVRCLLLKIADRQHNLNTISHLPNNKQIRM
                     AFETQAILLPTKEILGYDRSGVTVAKAIANWQAFLRRGHFKNLVQLKDDLYKKQFIDL
                     DCEMYDLVYSHTNAIIWQMTDKKLFEKMLADQLFNKTAEILSITSDGRNFRAEFQFSQ
                     GYTANPDLGVQLGISKFRV"
     gene            7206..8171
                     /locus_tag="PKV49_01915"
     CDS             7206..8171
                     /locus_tag="PKV49_01915"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HPL22400.1"
                     /translation="MTPINSLVSDLELDKEMIDCLKNRSLEQKFLYLDDGANFYYQAY
                     NKPTTPLPVDFSTEDYYDLVAGQFKKKQRFALVSLGCGDASSEKVMLKRLKKEGYNFT
                     YFGVDSSRQMLDLAVNNLAELKIDNQFLCADLVSEDFRDEIEQLTDDFDCRGFLFLGG
                     TISNVNQTNIIDSLYSILKKNDLLFLDIRIRNSLDNSEDLQLFNFYAKYLSDKKMAQW
                     LISPLRNVGIDPSCGKLSLEMVKEKSIGAILFKFFFLFNKKFVIRLRNEVIHFLPEER
                     VELLHIRAYYPDNLTSFFKEHDFKLVQSKTNKEIRDGFFVYQKIS"
     gene            8187..9794
                     /gene="dnaX"
                     /locus_tag="PKV49_01920"
     CDS             8187..9794
                     /gene="dnaX"
                     /locus_tag="PKV49_01920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPL22401.1"
                     /translation="MSTLYRQYRPQSFAEVFGQSHIKITLSEEIKRQQVASAYLFCGP
                     RAVGKTTIARLLAKALNCLNRPADSAEPCNQCVNCLAIAAGQSLDIIEIDAASQTGVD
                     NVRENIIASSRLAPSSGQYKVFIIDEVHMLSNSSFNALLKTLEEPPQKVIFILCTTEV
                     HKIPATVISRCERFDFKKISPTDMAKKLQYILKQEGWAADDAVLAAIAKNSQGHLRDA
                     ESLLGQLLAISQPAQDGPATLTMEDFYLLAPNSDMVLISQFLTALVDQKAIEAIGLVN
                     QLVTDGRDVRVFLQDLIELLRQLLLSKINPQLLPNYQAQWGDDDAFGLLDLRQRLSIN
                     RLVQLLQVFLQAFNESKEAFISQLPIELAIVKVLQPAEPPAASRPISPSQSPGGNQET
                     DKISEAINEIKYDATASQPPLLIDDLLNQWPTVLDMVKGRNAGLSVTLKACQPQLDGQ
                     QVCLLFRYKFHQDQVNQPKIKHLLEEILAEVYHRPVMIATKLLADLDIKLEKKQSSTG
                     STPVTPSDDSAINNLLEIFDGQIVGEA"
     gene            9769..10635
                     /locus_tag="PKV49_01925"
     CDS             9769..10635
                     /locus_tag="PKV49_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPL22402.1"
                     /translation="MVKSLAKPKQLKTSKHVGPSVGLALGGGGARGLAHIGALKILSQ
                     TGIKISYIAGTSIGAIIGAWLALGQPFDKLEELCLTFDRSKAWRQFVDIGNPRYSFLA
                     GQKIKKFVNQLFGRKQFTDCQIPLAIVATDLANGQEVILRQGSIAQAVLASIAIPGLL
                     PPVKLNRRYLIDGGSINITPASVVAQLGAQIIIAMDLSVQPSAHFQTKPTLFTTLVQA
                     YTIIREQSVKYQLAKIKSDRQRLIIVRPVFDDIIDTFQFDQTAKFIKLGEEAMRRRLP
                     AVRQALAKLGKI"
     gene            10653..10723
                     /locus_tag="PKV49_01930"
     tRNA            10653..10723
                     /locus_tag="PKV49_01930"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10685..10687,aa:Gln,seq:ctg)
     gene            complement(11105..11239)
                     /locus_tag="PKV49_01935"
     CDS             complement(11105..11239)
                     /locus_tag="PKV49_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22403.1"
                     /translation="MASDCEETSILMASKRARYMLVIIDFDPRKKQFIINNPGTKNNI
                     "
     gene            11293..11880
                     /locus_tag="PKV49_01940"
     CDS             11293..11880
                     /locus_tag="PKV49_01940"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPL22404.1"
                     /translation="MLDQILTILIAYQYWALLLIVFLAALFNIAPASSALIAAGALVA
                     LGYLDYSLVFLFGLVGSVVGDLLSYSLAFIYGQDVLIRLGFKRLLKSNKFQQINRVFT
                     AHSTRTIFVSRFFLTSFGPLINLIAGLARSDFKKFIICSLFGQIIYVTLLTGIGYVGA
                     RHWQYISNFYGYLALILVIIAVYWLYRRFFRRAVT"
     gene            12026..12550
                     /locus_tag="PKV49_01945"
     CDS             12026..12550
                     /locus_tag="PKV49_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22405.1"
                     /translation="MKDFNRGDGGGRFNGPRSLNNQHSSYPNHRFSRAIHSAICADCG
                     QPCEVPFKPSGDKPVYCYNCFGKKSAFDSGRLAKRHSARPSFQHNEQDRLASFINNQQ
                     QQLVILNTKLDNILRLLTAKPASDQSLERAAKDKEEHPVSKEVKKDVKKETTAKKVAT
                     PKKAVKKVVAKNKK"
     gene            12622..14082
                     /locus_tag="PKV49_01950"
     CDS             12622..14082
                     /locus_tag="PKV49_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL22406.1"
                     /translation="MAEEAILRFDQVSFEYVHKKPILDEASFVVRKGAKITLMGQNGA
                     GKSTIFGLIKGELQPKHGQVSVTAGATIGAARQMIAQEHLGLTIEDYFATAFDVVPGN
                     LKSRISKVLEAVNLDIPLDRIVGQASGGQQSRLLLAYSLIQDPDILLLDEPTNNLDRQ
                     GIDHLVTFLIMYDKTVIVISHDADFLNCFTEGVIYLDIFTHQTELYVGDYYSVVAEIQ
                     KRIEREVMQNAQLEKKIQDRKEKVNFFANKGGKMRKLAQKMREETAELEENKVDVRRE
                     DKTIRRFEIPAQENIKGDIVNLRKVMVIKNQQPVWCNIDKSLGRQSRLLVTGPNGIGK
                     SNLLRSLVNKSTADNTIAPQVTIGYYSQDFSALDFQQTVIDSLRSILAEGINEQEMRS
                     TAAGFFITGDLMERHIGQLSEGQKGLLSFARLVLMKPGLLILDEPTNHINFRHIPIIA
                     EAINNYQGAIIMVSHMADFVKDIEFSDQLNLGNLLA"
     gene            14147..15370
                     /locus_tag="PKV49_01955"
     CDS             14147..15370
                     /locus_tag="PKV49_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22407.1"
                     /translation="MLRAINNKLIRKNLTHNFRMIYFSRITLNSATQLLGLFLPIFLY
                     QFLDFSLFWVIVYYLISDLLYASLVAVGCRQIMNRFGIKRSLQLSVFFGAAFYAVFFL
                     INHYIAPHNQLVEGYQLWWLAPTIFFSLFFRLTHWIPFHTNVAKLTERHIRASQLSFM
                     EATMMALGAVMPIVGGLALEYLGYSTLFFLAVSIYLISLIPFSQLPQVKEKFSWTYRE
                     TWRHFLSKKMRGSVLAYAGDGAENVVGSIIWPIFIWQLLSGNYLQVGAISSVIILATI
                     VLQIMVGNLIDNLPSRRWLRYGTVLYSFGWVLKALINSAFQVFLFSTYHNLSRVFART
                     SFDALNYDIAADQGHYVDEYTVIREMAIVFGKALMCIFIILISPFLALRYLFLVAALA
                     SLLMSLLKPDQLVDN"
     gene            15499..16239
                     /locus_tag="PKV49_01960"
     CDS             15499..16239
                     /locus_tag="PKV49_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPL22408.1"
                     /translation="MKQAHFIMSLAALLAVTAIVITALVTTNKTNNQDRFSSNGSGTV
                     YAKADIANIQVGFKSGVKKTAAEATTESTAKMNNIIAALQKLGIEEKDIKTSNYNLSP
                     SYNWTNTKGQELVGYEVSQNLTLKVRDLNKIGDVIARTTEQGANQIGNVSFTIDDEFE
                     LRNQARELAIQQAKEKAALIAKQSGMKLGEIKGVYENTDLERQQAAYSNTKMMIDSSI
                     AGAELATPSVQVGQNEIRAEVTLMYEVK"
     gene            16265..16495
                     /locus_tag="PKV49_01965"
     CDS             16265..16495
                     /locus_tag="PKV49_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HPL22409.1"
                     /translation="MSDLIQVINTNPYLNLLIASWVLSWKGVALWKAARRKQKWWFIP
                     LLVINTVGLFEIVYIFIFSKIKPKRPAKDNCV"
     gene            16568..18004
                     /locus_tag="PKV49_01970"
     CDS             16568..18004
                     /locus_tag="PKV49_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPL22410.1"
                     /translation="MKKYSFFDYLKKKQEFEQARRAGQKFLDSDQDRLTDFEEINLYG
                     TDPHNPDSDFDGVSDGDEVMANRNPLGAGKLRDLFVPCAQNDYAPEILRPRRIAFHVV
                     SLLAIKLILMSFIIFYPLSAWLSPDISLNEARKVIDLTNELRKKLKLEVLVENSRLTQ
                     AAWQKTQDMLINQYFAHLNPSGQDLSYWINKNGYKYSIIGENLAMGYVTAKDVVTAWS
                     KSPTHYRNIVDRNFKETGVAIGDGRFKEVDTVFMVQYFGRPALVKNSSAKAAAAPSKR
                     TTAKKNARQLASAAKSVKGDRAYSIESAEINKFKTGTISIKKDAFSKTATIKTEVVLP
                     AETIAATVIINNRELELTKSSAEDYWSGVALISSDEEKEIFSPLVPATLLTEDNLGQQ
                     NIYQLDWNEIKPIKTSIWEKYFLFKRNPTNNMLPILIFGNFYFKILFVVFSLAAILNI
                     VVKIKKQKLELILGSFGIIVLLAIFILF"
     gene            18110..20716
                     /locus_tag="PKV49_01975"
     CDS             18110..20716
                     /locus_tag="PKV49_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HPL22411.1"
                     /translation="MKRIASNFKRGTFLCSAICLLANFALLALIVPHSLVRAASIDAV
                     SLKGIEEAPFFKDNGFVVNTKPAPDFGGKYWLEKDKSIPNYTIDLAKGAATNLSDGSY
                     PIMLVKAPTEDLAKYYEQEFGLSDEYIKGVIDGHNPFAFINISKGRARLIRNEKIDSK
                     SLDNNNNQGQIDDHLALTVSYKFPVGEYSFLLTNKNSQLAKWQFRVVDNYPAADIFAV
                     ETAYNSLTLGSNKIYENINLPTTYKDTEIKWTSSDTNVMTNGGIINRSDTEDLPVTLT
                     ARISSGSFFMDKTFDLIVVKNDPYLEINNFTANGKSMINKENSLFIFQKQDGIKKYII
                     DFASGYKTNLQQGKYAVRFDNLSEESQDKLIKYYSKDNLSDEDSRLIAQGKEPHAYIQ
                     KDKNGQLRLISSFQDSANSKSKPIIGIDQKNSFLIREDYPDGLYDIVADLDGFDGHHK
                     IKWQVEVAPVPVIIDPDSALVQADYDALTLASPVTEDFDLKTSGINGSSISWSSNNSG
                     AIAINGNIAQVTRPEFSAGDTVVQLTATLTKGSITRTKTFPITVLKNEQVVPTTGGGG
                     GGGIAHSLIPQSVSAINVPLSLVSSQDGVLTQSLSNGLQARLVVSPNSFVGGVTFIIT
                     EGPEDNALGGRHFILTAVDSNGNPVRNFTNNLNITFSGLPLPDNIAGVGVYYYDEALL
                     SWVLVPGAIFDQAGGSVNFFVNHLTKFAVFSEQPVLQQVLGETTYADGTLLRGSDRRI
                     FVVVDGKLQHILTLKELAQYANQEILDVEDSIIASYPKASPKAVLGVRKYNFNRDLKT
                     GMTGDDVRELQRYLNNNGYVLAKTGPGSAGQETKKFGRATREALIKFQKANKINPAVG
                     YFGTITRKAVNK"
     gene            20811..>21292
                     /locus_tag="PKV49_01980"
     CDS             20811..>21292
                     /locus_tag="PKV49_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22412.1"
                     /translation="MLSARLIKPKWITGFSWPLFLAAMLLIIPSLIIAWLSISRQDYF
                     QQHLENISFSDYRLLALCFGCYLLAWLALIIIKPANNNYFLWLTAAIIFIISLVTVPL
                     LSRDAAAYALNADMVYYGHFNPYIEPINLGRQSFQLGSLWWLNHPSPYGPLFLLIIAL
                     P"
BASE COUNT         6035 a         3935 c         4554 g         6768 t
ORIGIN      
        1 catcatacta atgaaattgc ccaatcagag gcggccactg gtcagccgtt tttccgttat
       61 tggttacaca atgctttttt aaacattgtc ggcggtaaaa aaatggccaa aagcgccgat
      121 aattttttaa ccctggctaa agtagtagcc gatctcaaga tatcgccgct agcttatcgc
      181 tatgcctctt tgcaagttca ttatcgcaag ccgatggaat atagccccga tagtttagtg
      241 gctgccaatc aggctcttta tggattatat cggcaagtgg ctgatttggg ttcagcaatt
      301 ggtcaagtgg ctagtttttt tcaagctcaa tttcaacagg cgattaataa tgatcttaat
      361 gccgctcagg cgctggcggt tacttttgct gttattaaca gtcaattgac ctcggccgat
      421 aaattagcga caattttgaa ttttgatcaa gttctgggtt ttaatctagc caaaagtcgt
      481 caaatattgg ctgttgacgc ggcggctatt cctcaaaaca ttcttatgat gtttgagcaa
      541 cgccaaatcg ctcgtcaagc tggtgatttt agtctagccg ataatttgcg ccagcagttg
      601 ttagcggctg gctacaagat tgaagacagt cccgacggca gtcaactgcg accggtttaa
      661 atattatggt taatttttgg tctcaacttt ctaagcccat tttggtctta gcgcctttgg
      721 ctggcgttac tgacagcgct tttcgtcaga tttgcaagag ttatggggcg caagtagtgg
      781 tcagtgagat ggctagtgtg gccgctttgg ttcatgatca gcgcaaaaca ctagaaatgt
      841 tagccttcca gccaatagag cgcccttata ttgtccaact gtttggtagt gatccccagc
      901 aatttgccgt tgccgctcaa ataattacag aaaaaataca gccggacgga ctggatatta
      961 attttggctg tccagtgcct aagataatta agcagggcgc cggcgctgct ctcggcgctg
     1021 acttatcgcg agccagagag gtggttaggg cggttttaga taacactgat cgacccctat
     1081 ctattaaatt aagagcgttt aatcagggta tcagcgctca gcaatttgtg gctaatttgg
     1141 ctgatttacc aatcgccgcc gtggctgttc atgggcgcag tttagcggct ggctttagcg
     1201 gcttcgttga tccgacggcc attgcggctt gtcggtctta tgtcaatggt caattattag
     1261 ctaatggtgg gataattgat ttatttagtg ctcaagcgat gttagccgct agccaagccg
     1321 atggtttaat gattggtcag ggggcgctca gtcggccatg gatttttcaa gaaattgccc
     1381 agcaagtaaa tattaatttg tcatggtcag agcttagcca gctgatgctt cgtcatgcta
     1441 agttagttgt tgaaaataag ggacaggccg gtctgatgga gttgcgtaag tttttggcct
     1501 ggtatgttaa gggttggccg caagccaaaa aaatccgtca acaattagtg gccattaaat
     1561 caatagctga tttagagact attatatcca atttttgcgt ttaaaaatta ggaaaatcgt
     1621 tactgtgctt aagaacatta taaataatat tttccaaaat ccaagcgggt cgcctataaa
     1681 gggcatagcc aatattgtgt tcataccaaa aatggcggca attaaattca gtggatagag
     1741 aataaccgtc atcatagtta aggttttcat aatacggttc atacgatcgt tgagcagcga
     1801 ttcattggtg tcatttaaaa cctcaatcat ttcccgttgg ttttccagat tttcccaaat
     1861 tctttttgaa tgttcaacca aggttgaata atattttttt aattcagcgg ccggcacgag
     1921 gctgcttttc atttcagtca ggctctgtaa aatattttta tgattttgca aaattttacg
     1981 gattttaata atattatgac gcaaggtcaa tatttcacta gctgtgtcgc gctgcttgct
     2041 gtcaagacta aaaatagtgt tttgggcatg agtgattctt ttgctatgac tatccaaaac
     2101 ttcataacag tatttgatta gacgatccag tatttcataa agcaggactg acgatgattc
     2161 cagttcatag gccagcagcg agttctcatc ttttttgcaa atattaaaaa agtccatcag
     2221 cggcggcaga tcaccattat ggatagttac taaataacca tgacccataa agaattctac
     2281 ttcagccggc aagactaagc catctttgat tatgggaaaa tgaagaataa taaaaatata
     2341 gttagtttct ttttctataa aagctcgttc cgccagactt ttggccatcg aagctttcaa
     2401 gtgggtgatg gtaaagtgtt gtcgtttgag caagtattcc aattctggtt tgccagcact
     2461 attgacattg agccagctca gtgatccagc agttttagga ttgtcaataa tcaattcttc
     2521 aatttttttg gaaacttgta aatatttacc cataatttta gctaatgatt attaaagtaa
     2581 ttatagcata aagacgacaa cggccacaac tttggattag tggctcaatc agttggttta
     2641 tggtataatg atttcaaata agcaatcatt ttttttatgc ctcaagaaca attgaagcct
     2701 agttttgagc agatgccgct gaataatagt caggaacggc agccgactgc tgaaaaaata
     2761 accgaatcag ctattagtga agcggcgcca gtcacggtag agcccgtagt taattcagcc
     2821 gcccagcagc caccagccat aacagctagc cagccgacta aattgacagt tcgtcaagaa
     2881 gctattgatg atattttaga agacggctta gctgacattt atcagaatct cccacccgat
     2941 aagcagcaag agctcaagca agctggtgaa cggacggttc atcagattga tcggttgcta
     3001 gatcatgcca agtcgcagct aactaagata atcagtttga ttcgccgctt cttagctata
     3061 atccccggtg ttaatcggtt tttcttagag caagaagtca aaattaaaac tgacaagatt
     3121 atggctttaa aagataaata atcattatga actggttatt tgatttcttt aatgacagca
     3181 atgacttatt ggtcgttttt ttaactatct tcattacagt tattgctgtt atggggctaa
     3241 ttattttatg gcgttattta gtttggcgct tgtttagtcg gcatcgctat tttgatcaag
     3301 tagtttattt agttaaattg ccgaaagatc gccaacgaga ccaaaatcag aattttactg
     3361 ttcagcagat gcgcgaagaa attgcctgcg ccgagaccat ttttgccagt atcggtggct
     3421 tgcgggctca acgtggcttc ggagtttggt tgtggggacg cgatgatcat ttttcttttg
     3481 agattgttgc caatcaagga ttgattgctt tttatgttgt tagtccgcgg cggttagctc
     3541 gctatttgga acaacagatc aacgctcatt atcctgatgc tatgcttgaa gaagtggttg
     3601 attataatct ttttaaacca cagagttatg tggccgtggg tagtttaaag acgactcgtc
     3661 attatgcctt gccaattaaa acctacgatc aacaagaagc tgatttgatg aattctcagc
     3721 ttaatgttat gtctaaatta tctactgatg atagtttggc aattcaatat accgtgcgta
     3781 gcgcctatgg tgtctggcac ggtacagtga aaaaaatcgt tgaattatcg cacaagaaga
     3841 aatcagtatc agcggcactc aaacatgggt cgtttgatag ctggtcagat tttattaaag
     3901 aaaattttgg cagtaagaaa aatcaggaac aggccaatgc taagcaattg gataagcagt
     3961 tgactgccat ggagggcgat atgctcaagt taatggaaga aaaaaatgcc aaagccggct
     4021 tagatgttaa tgtgcgatta attgcttgtt ctaatagtca agcgcattcc gataatttgc
     4081 ttaatgactt agctaacgct ttgcatcagt ataattatta tgaatacggc aatagtttta
     4141 gcaataagcc gcgccggcgg ccggccaagc aattaattga acagtttatt tatcgtcgct
     4201 ttgatgataa ggctagtttt ttgttaaata ctgaagaatt atccagttta tttcattttc
     4261 cgctacaaaa cgccgaaact cctaatattt tatggctgac cgctaagcaa gcggccgctt
     4321 cagccgatgt acctaaccaa ggcttattat tgggttataa tatttttcgc ggtatcaaga
     4381 aagatatttg tatcaaggaa gatgatcgtt tacggcatac atatattatt ggtaaatccg
     4441 gtgttggtaa gtcagttttg ttagccaata tggctatcca ggatgttttg aatggcgctg
     4501 gcgtttgcgt cattgatccg catggcgatc tggttgatga tgttttggct agagtgccgc
     4561 cagagcgggc cgaagatgtt atcttattcg cgccagctga tttagagcgg ccgatgggtt
     4621 tgaatttatt agagtttgat gccaaatacc cggaacagaa aacctttgtt atcaacgaaa
     4681 tgattggtat ttttgataag ctctatgatt tgaaatcaac cggcggtccg atttttgaac
     4741 aatatatgcg taatgccatg ctgctagtca tggccgatcc cgaatccggc tcaactttaa
     4801 tggaaattcc tcgagtttta gctgatgctg agttccgcaa tctcaaactt tcccgctgtg
     4861 ccgatcaaac agtcgttgat ttttggcgca aggaagccga aaaagccggt ggcgacgccg
     4921 ctttggccaa tgttgttccc tatatcacct ccaaattgac ctcttttatt tctaatgaca
     4981 tgatgcggcc gattatcgct cagcaaacca gcgcttttaa tttccgcgac atcatggata
     5041 aacgcaagat tctattggtt agcttgccta aaggattagt cggcgagctt aatgcttatc
     5101 tattgggcat gattttggtt ggcaagctgt tgatggctgc tttgtcgcga gcggatatgc
     5161 ccaaagaaga acgccgcaat tttttcttat atattgatga gtttcaaaat tttaccacca
     5221 attcagtttg tcagattctg tcagaagcta gaaaatatgg actcggcttg attatcgccc
     5281 atcagtatat cggccaattg gtggcccgcg gcgacaccgc tattaaagac gctgtttttg
     5341 gcaacgtcgg cacaattatt tcgtttaaga ttggttccga agatgccgaa gttctgcaaa
     5401 aagaattcgc gccgactttt gatgcctatg atttgattaa tgttgaaaaa tatactgctt
     5461 atatcaaatt gttaattaac aacactgctt cacggccgtt taccttaaaa acgccatggc
     5521 cgctggctgg ccaagaaagc ttggatctgg ctggcaaaat tcgtcagtta agccggctca
     5581 agtatggtca ggatcgatct ttggtggagg cggaaatcag ccagcgtggc aaatttaatt
     5641 tatagcttat gtccagttca accgataaaa accaagaccc agttaataat gatgtttggc
     5701 aaccggatca ggccattgtt tttaatcagc ctgatcaacc ccagccgact tcaagccatc
     5761 agtcgccagt taaggacagc cagccgcttg atcaatcagg cgctttgcaa aatatcgcca
     5821 gtcgtttaga attattggcc caagaattgc gctcaattat tcagacgacc agtcagccat
     5881 ctttggctga tcatcaagta attgactcct taacccagcc aatcgatgat ggtgataaaa
     5941 ttattgaagg ttattttgac ggcgagcaga tgattgccac cactggccaa gcctatgccg
     6001 ttccagctaa ttatgcctcc aaatcaaagt tggttgctgg tgatagctta aaattaacca
     6061 tcggtccgcg tgggcacttt ctttataagc aagttagccc ggtagaaagg cggcgcttgg
     6121 ttgccagctt ggaacaagcg ccagatggca attattacgc cgtttatgaa catcagcgct
     6181 ggcggttgct caaagcgagt gttagttatt ttcgggcgca gcctggcgat cggattgcca
     6241 tattgattcc gcaggattta ccagccaatt ttgccgcctt agagaattta gttgccgaat
     6301 aaaaattatg ctaggatggt ggccggacaa aaaaataatt aacagtttta agcagctgac
     6361 tgcggccgac gaagaattat taaagaaggc ctggaatttg gctcgtcatt tgcatgccgg
     6421 tcaaaaacgc aagtctggtc aaccctattt tactcatccg gttgccgtgg ccgaaatttt
     6481 ggctgagaaa tttgccgatc cggttctgac ggcagcggcc ttgctccacg atacggttga
     6541 agattgcccg gattgtctga tgggtgatat ctatcgcgat tttggcaccc aggttggctt
     6601 cttggttgat tcagtcacta aagatcgcag taatttttat tgccaaggcc aagaatttga
     6661 cgatgatatt gaacggctgt tgtgggccgg cggccaagat gttcgttgct tattgcttaa
     6721 aattgctgat cgtcagcata atctgaacac aataagccat ttgccaaaca acaaacagat
     6781 tagaatggcc tttgaaactc aagcgattct tctgccgact aaagagatct tgggttatga
     6841 tcggtctggg gtaactgtcg ccaaggctat agctaattgg caggccttcc tgcggcgcgg
     6901 tcactttaag aacttggttc aattgaaaga cgatttatac aaaaaacaat ttattgatct
     6961 ggattgcgaa atgtatgatt tggtttatag tcataccaat gctattattt ggcagatgac
     7021 tgataaaaag ttgtttgaaa aaatgctggc cgatcaatta tttaataaaa ccgctgagat
     7081 tctatctata accagtgacg gaagaaactt tcgagccgaa ttccaattta gccaaggcta
     7141 tacagccaat cccgacttgg gcgttcaatt gggcatatct aagtttcgcg tttaattata
     7201 aaattatgac acccatcaat tcattggtct ccgatttgga actggacaaa gagatgattg
     7261 actgtcttaa gaacaggagc ttggagcaga agtttcttta tctagacgac ggggccaatt
     7321 tttattatca ggcttacaac aagccgacaa cgccgctgcc cgttgatttt tctaccgaag
     7381 attattacga tttggtcgcc ggccagttca agaaaaaaca gcgtttcgct ttggtgagcc
     7441 taggatgcgg tgatgcttcc agcgagaagg tgatgttgaa gagactcaag aaagagggct
     7501 acaactttac ttatttcggt gttgattcat cgcgacagat gttggattta gctgttaata
     7561 atctggctga gctcaagatt gacaatcaat ttttatgcgc cgatttggtc agcgaggatt
     7621 ttcgtgatga aattgaacaa ttaactgatg actttgattg tcgggggttt ttatttcttg
     7681 gcggaactat cagcaatgtc aatcaaacta atattattga tagtctttac agcattctta
     7741 aaaagaacga tttattgttt ttagatatta gaattcgcaa cagcttagat aactccgagg
     7801 atttgcaatt atttaatttt tatgccaaat atttatccga taaaaaaatg gctcaatggt
     7861 tgattagtcc tttgcgcaat gtcggtattg accctagctg tggcaagctc agtctggaaa
     7921 tggttaaaga aaaatctatc ggcgccattt tatttaaatt tttcttttta tttaataaaa
     7981 aatttgttat ccgactacgc aacgaagtta ttcatttttt accggaagaa cgggttgaat
     8041 tattacatat ccgcgcctat tatccggaca atcttacttc ttttttcaaa gagcatgatt
     8101 tcaagttagt ccaatcaaaa actaataaag aaattagaga cggcttcttt gtttatcaaa
     8161 aaatcagtta ataaatcatt ttcgttatgt ctacccttta ccgccaatat cgtcctcaga
     8221 gttttgctga agttttcggt caaagccata tcaaaataac attatctgaa gaaattaagc
     8281 ggcagcaggt ggcttcagct tatttatttt gcggtccgcg ggcggtcggt aagacaacta
     8341 ttgccagatt attggctaag gcgcttaatt gtttaaatcg gccggctgat tcggctgaac
     8401 cttgtaatca gtgcgtcaac tgcttggcta tcgccgctgg tcagtcgcta gacattattg
     8461 aaattgatgc ggccagccag accggtgttg ataatgtccg agaaaacatt attgccagca
     8521 gtcggctagc gcccagtagt ggacaatata aagtcttcat tattgatgaa gtccacatgc
     8581 tgtccaattc gtcattcaat gctttgttaa aaactttgga agagccgcct cagaaagtga
     8641 tttttatttt atgcacgacc gaagttcata agattcccgc cacggttatt tcccgttgtg
     8701 agcgttttga cttcaagaag attagtccga ccgatatggc gaagaaattg caatatatct
     8761 tgaaacagga gggttgggcg gctgatgatg ctgttttggc ggccattgcc aagaattctc
     8821 agggccacct tcgtgatgcc gagagcttgc tcggacaatt actggctatt agtcaaccgg
     8881 ctcaagatgg accagccacc ttaacgatgg aggattttta tttattggct cccaatagtg
     8941 atatggtctt gattagccag tttttaaccg ccttagtaga ccaaaaagcg attgaggcca
     9001 ttggcctggt taatcagctg gtgactgacg gccgcgatgt tcgagttttt ttacaagatt
     9061 taattgagct tttacgtcaa ttgttattaa gcaaaatcaa tccacaatta ttacccaatt
     9121 accaagcgca gtggggcgat gatgacgcct tcggcttgtt agatttgcgt cagcggttgt
     9181 cgattaatcg tctggtgcaa ttattgcaag tttttttgca agcttttaat gaaagcaagg
     9241 aggcttttat cagtcaatta ccgattgaat tggctattgt taaagttttg cagccggctg
     9301 agccgccagc cgccagccgt ccaataagcc catctcagtc gccgggtggt aatcaagaga
     9361 ccgataaaat cagcgaagcg attaatgaga tcaagtatga tgcaacagcc agccagccgc
     9421 cactattaat tgatgattta ttgaatcaat ggccaacggt tttagatatg gtcaagggtc
     9481 gtaatgccgg cttatcagtc acacttaagg cttgtcagcc acaattagat ggccagcaag
     9541 tctgtttact ctttcgctat aaatttcatc aagatcaagt caatcagcct aaaatcaaac
     9601 acctgctaga agaaattttg gccgaagttt atcatcggcc ggtcatgatt gccactaaat
     9661 tattagctga tttggacatt aagctggaaa agaaacaatc atcgactggt tcaacgccag
     9721 ttacgccgtc agatgattca gcaatcaata atttattaga aattttcgat ggtcaaatcg
     9781 ttggcgaagc ctaaacagtt aaaaacaagc aagcatgtcg gcccatcggt cggtttggct
     9841 ttgggtggtg gcggcgctcg cggattggct catatcggcg ctcttaaaat cctgtctcaa
     9901 actggtatca aaattagtta tatcgccggc actagtatcg gagcaattat tggcgcctgg
     9961 ttagcccttg gccagccgtt tgacaagtta gaagaacttt gtctgacttt tgatcgttct
    10021 aaggcctggc ggcaatttgt ggatattggc aatccacgct attcattttt agccggccag
    10081 aaaattaaaa aattcgtcaa ccagctgttc ggccgaaaac aatttacaga ttgccaaata
    10141 ccattagcca tcgtggccac cgatttggcc aatggtcagg aagttatttt acgtcaaggc
    10201 agtattgctc aagcagtttt agctagcatt gctattcctg gactattgcc gccggttaaa
    10261 ctcaaccggc gttatttgat tgatggtggt tcgattaata tcacgccggc tagcgttgtc
    10321 gctcagctcg gcgcccagat tattattgcc atggatttat cggttcagcc atcagctcat
    10381 tttcaaacca agccgacctt gtttacgacc ttggtccaag cgtatactat tatccgagag
    10441 caatcggtta aatatcaact cgcgaagatt aagagtgatc gccagcgttt aattatcgtt
    10501 cgtccggttt ttgatgatat tattgatact tttcagtttg atcagacggc aaaatttatt
    10561 aagcttggcg aggaggcaat gcgccgtcgt ttgccggctg ttcgtcaagc tcttgccaag
    10621 ctaggcaaga tatgattaaa taagagcatt cttcggggat cgtctaatgg taggacgaca
    10681 ggttctggcc ctgttaatcg aggttcaaat ccttgtcccc gagcactact accgttttag
    10741 gaaaaagaca agcgtcgtca gcaataataa agacaggcta aagcttgttt ttttgttgca
    10801 ctataatcat aattaatatt taatttgata taaaatcatt gacaaattag cagaactatg
    10861 atataattta atatcatcaa attgtatttt aaaaataaat aaaaaaaaga aggaggtgga
    10921 acatgttact tcgaatgttc ctcgccatcc tgccatttat attggcttgg gcccttgttt
    10981 ttctgtttgt caagatgttc gacgaccacg acatctaaac taaaccacct caaccccaag
    11041 gggggagagg gaataacatt taaaaagagt ttaccagttc tctaaaaact ggttttttct
    11101 ttggctaaat attatttttg gtgccgggat tgttgatgat aaattgtttc ttgcgcggat
    11161 caaaatcaat aattaccagc atatatcgtg cccgtttact tgccattaag attgaagttt
    11221 cttcacagtc actggccata acagcgatta agtggtaaag ttttaatggt tattatttat
    11281 cattactggt tcatgctaga ccaaatacta actattctta ttgcctacca atattgggct
    11341 ttactactta ttgtttttct tgccgcctta tttaatattg cgccggctag ctcagccttg
    11401 attgccgctg gtgcgctagt tgctttaggt tatctggatt attctttagt ttttttgttt
    11461 ggtctggttg gttctgttgt tggtgatttg ttatcctata gcttggcttt tatttatggc
    11521 caagatgtat taattcgtct tggttttaag cgcctgctca agtcaaataa atttcaacaa
    11581 attaacaggg tttttacagc tcattccacg cgcaccattt ttgtcagccg ttttttctta
    11641 accagcttcg gtccgcttat caacttaatt gccggcttag cgcggtccga ttttaaaaaa
    11701 tttattattt gcagtttgtt cggccagata atttatgtta ctttattgac tggcattggt
    11761 tatgtcggcg ctcgccattg gcagtatatt tctaattttt atggctatct agccttaatc
    11821 ttggtgatca tagctgttta ttggctgtat cgtcgttttt tccgtcgagc cgtaacttga
    11881 ttattattgt tataaatagt tttccgccaa gcgaacatca atagaggcga tgctgagatg
    11941 gctggtattg atgaagttca ggttaaaatt ttaagcacaa tgtggtaaaa tactattaat
    12001 taacttaatt aaataattag ttgttatgaa agattttaat cgaggtgatg gaggtggtcg
    12061 attcaatgga ccaagaagtt tgaacaatca gcatagctct tatcctaacc atcggtttag
    12121 tcgagctata cattcggcga tttgcgctga ttgcggccaa ccttgcgagg tgccgtttaa
    12181 acctagcggt gataaaccag tttattgtta taattgtttt ggtaaaaaat ccgcttttga
    12241 cagcggtcgt ttggccaaac gacattcagc ccgacccagt ttccagcata atgaacaaga
    12301 tcggttggct agctttatca ataaccaaca acaacaattg gtcatactta atactaagct
    12361 tgataatatt ttaagattat tgactgcaaa accggctagc gatcaatctc ttgaaagagc
    12421 cgccaaagac aaagaagaac atcccgtcag taaagaggtt aaaaaagatg tcaaaaaaga
    12481 aactacggcc aagaaagttg ctactccgaa aaaagcagtc aaaaaagttg ttgctaagaa
    12541 taaaaaataa attggtagat aaaaaggagc cggccttggc tcctaattga agattaaaga
    12601 gtttttaaag actttaaaga catggctgaa gaagcaattt taagatttga tcaagtcagt
    12661 tttgaatatg ttcataaaaa gccgatatta gatgaggcta gttttgtggt ccgtaagggg
    12721 gctaaaatta ctctgatggg ccaaaatggc gctggtaaga gcactatttt cggtttaatt
    12781 aagggtgagc ttcagccaaa acatggccaa gtttcagtga cggctggcgc gaccattggc
    12841 gcggctcgcc agatgattgc tcaggagcat ttaggattga ccattgagga ttattttgcc
    12901 accgctttcg atgttgtccc tggtaatctc aagagccgga ttagtaaggt tttggaagcg
    12961 gttaatttgg atattccttt agacagaatc gttggtcaag cctctggcgg ccagcaatcg
    13021 cggttgcttt tggcctattc tttgattcag gatccggaca ttttgctatt agatgaaccg
    13081 actaataact tggatcggca ggggattgat cacttagtaa cttttttgat catgtatgat
    13141 aaaacagtta ttgttatttc ccatgatgcc gattttttga attgttttac tgaaggcgtt
    13201 atttatttag atatttttac tcatcaaacc gagctttatg ttggcgatta ttattccgtt
    13261 gttgcagaaa tccaaaagcg gattgagcgg gaagtgatgc agaacgccca attagaaaaa
    13321 aaaattcagg accgcaaaga aaaggttaat ttttttgcca acaagggcgg taaaatgcgc
    13381 aaattagccc aaaaaatgag agaagaaact gcggaattgg aagaaaacaa ggttgatgtt
    13441 cggcgtgaag ataaaactat tcggcgtttt gagattccgg cccaagagaa tatcaagggt
    13501 gatattgtta atttgcgtaa agtgatggtt attaagaatc agcaaccggt ttggtgcaat
    13561 attgataaat cgctgggtcg tcagagtcgt cttttggtta ccggtcccaa cggtattggt
    13621 aagagtaatt tgcttaggag tttggtaaac aagtcaaccg ccgataatac catcgctcct
    13681 caggtgacta ttggctatta tagtcaagat ttttcagctc tggattttca gcagacagtt
    13741 attgattcat tgcgtagtat tttagctgag ggcattaatg aacaagaaat gcgctcaacc
    13801 gctgccggtt tttttataac tggcgattta atggaacgcc acattgggca gctatctgag
    13861 ggacaaaagg gtttactatc ttttgctcgt ttagtcttaa tgaaaccggg tttattaatt
    13921 cttgatgaac caaccaatca tattaatttc cgtcatattc cgataattgc cgaggcgatt
    13981 aataattatc agggggcaat tattatggtt agtcatatgg ctgattttgt taaagacatt
    14041 gaatttagcg atcagctcaa tttagggaat ttactagctt aaggccaact atttggctgg
    14101 ccaaactata atatgccaag tagactcact agattaaatg tttagtatgt taagagcaat
    14161 taacaataaa ttgatcagaa aaaatttgac tcataatttt cggatgattt attttagccg
    14221 aattaccctt aattcagcaa ctcaattatt gggtctcttc ttgccgattt ttttgtatca
    14281 atttttagat tttagcctat tttgggttat tgtttattat ctaatttctg atttgctgta
    14341 tgctagtctg gtggcggttg gttgtcggca aatcatgaat cggtttggta ttaagcggtc
    14401 attgcaattg agtgtttttt ttggcgccgc tttttatgct gttttctttt taatcaatca
    14461 ttatattgct ccccataatc aattagtgga aggttatcag ttatggtggt tggcgcccac
    14521 cattttcttt tctttatttt ttcgtctaac tcactggatt ccttttcata ctaatgtagc
    14581 taaattgacc gaacgccata ttcgggctag tcagcttagt tttatggagg ccactatgat
    14641 ggccttaggc gcagtgatgc caattgtcgg cggtctagcc ctagaatatc ttggttatag
    14701 tacattattt tttttggcgg tcagtattta tttaatttct ttaattccct tcagccaatt
    14761 accgcaagta aaagaaaaat tttcttggac ttaccgagaa acctggcgac attttttgtc
    14821 caagaagatg cgcggtagcg ttttagccta tgctggagat ggtgcggaaa atgtagtcgg
    14881 atccatcatt tggccaattt ttatttggca gttattgtct ggtaactatt tgcaagtcgg
    14941 ggctatttct tcggttatta ttttggcgac tattgtttta caaattatgg ttggcaactt
    15001 gatagacaat ctgcccagtc gtcgctggtt gcgctatggc actgttcttt attcttttgg
    15061 ctgggtgctg aaagctctaa ttaatagcgc ttttcaggtt ttcttgtttt cgacttatca
    15121 taatttgagc cgagtttttg ctcgcacctc ttttgatgcc ctcaattatg atatcgccgc
    15181 cgatcagggt cattatgttg acgaatatac ggttattcgc gagatggcca ttgtttttgg
    15241 caaggcgtta atgtgtatct ttattatttt aattagtcca tttttggctt tgcgttattt
    15301 attcctagta gccgctctgg cttctttgct gatgtcatta ttaaagccgg atcagctggt
    15361 tgataattag atcagcgctt atttttttat tctgtcagtt tcgttattta tttcaatgaa
    15421 gcgaattaag acattaatac ccaacttatt tagttttgtg ctataatatt agcgtaacta
    15481 ttaatcatga ataaatttat gaagcaagct cattttatta tgtctctagc tgctttattg
    15541 gcggtgacgg ccattgttat tactgcctta gttaccacca ataaaactaa taatcaggat
    15601 cgcttctcgt ccaacggctc gggaacagtt tatgccaaag ccgacattgc caatatccaa
    15661 gttggtttta agagtggtgt taaaaaaaca gcggctgaag ccacgaccga aagcaccgcc
    15721 aagatgaata atattattgc cgctcttcag aaattaggta ttgaagaaaa agatatcaag
    15781 actagcaatt ataatcttag tcctagctat aattggacca ataccaaggg gcaagaattg
    15841 gttggttatg aggtgtcgca aaatttgact ttgaaagttc gtgatttgaa taagattggc
    15901 gatgttattg ctcggaccac tgagcagggc gctaaccaga ttggcaatgt cagttttacc
    15961 attgatgatg aatttgagtt gcgcaatcag gctcgggagt tggccattca gcaagctaaa
    16021 gaaaaggccg ccttgattgc caagcaatca ggcatgaagc tcggcgaaat caagggtgtc
    16081 tatgagaaca ctgatttgga gcgacaacag gcagcttatt ctaatactaa aatgatgata
    16141 gatagctcta tcgcgggcgc tgaattagcc acgccatcgg ttcaagttgg ccaaaatgaa
    16201 attagagctg aggtgacctt gatgtatgaa gtcaagtaat tttattaatc gttcaacaat
    16261 ttttatgtcc gatttaattc aagttattaa tactaatccc tatttaaacc tattgatcgc
    16321 ttcatgggtt ttgtcctgga aaggcgtagc tctttggaaa gccgctcggc gtaagcaaaa
    16381 atggtggttt attcctttac tggttatcaa tactgtcggt ttatttgaga tagtttatat
    16441 ttttatcttt agcaaaatta aaccaaaaag accagcaaaa gacaactgcg tctaaaggag
    16501 catttgtctt gacagtagtt ggtttttatg ttatagtttt aggctaaaaa aatacggtta
    16561 aaatataatg aaaaaatata gtttttttga ttatttaaag aaaaaacaag aatttgaaca
    16621 agctcgtcga gctggtcaaa agtttttaga ttcagatcaa gaccgtttaa ctgattttga
    16681 agaaattaat ttgtatggca ctgatccgca taatcctgat tctgattttg acggagttag
    16741 cgatggtgat gaagttatgg ccaatcgtaa tccgctcggg gctggaaaac ttcgtgattt
    16801 atttgttccc tgcgctcaaa acgactacgc accagaaatt ttaaggccgc gaagaattgc
    16861 ttttcatgtt gttagcttgc tggctatcaa gctgatctta atgtcattta ttatttttta
    16921 tccactttcc gcttggctaa gccctgacat atcgcttaat gaggcgcgca aggttattga
    16981 tttaactaat gaacttagaa aaaaattaaa acttgaagtt ttagttgaga atagtcgctt
    17041 aactcaggct gcttggcaaa aaactcagga catgttgatt aatcaatatt ttgcccacct
    17101 taatccgtca ggccaagatt taagctattg gattaataaa aatggctata aatattcaat
    17161 aattggcgaa aatttggcta tgggctatgt gacagccaag gatgttgtta cggcttggtc
    17221 gaaaagccca actcattatc ggaatattgt tgatcgtaat tttaaggaaa ctggcgtggc
    17281 cattggcgat ggtcgtttta aagaggtgga tacggttttt atggtgcagt attttggtcg
    17341 ccccgctctt gttaaaaaca gctcagccaa agccgcagcc gctccctcca aaagaaccac
    17401 tgccaaaaag aacgcacggc aattagccag tgccgccaag agcgttaagg gtgatagagc
    17461 ttattctata gaatcggcag aaataaataa gttcaaaaca ggcaccatta gcattaaaaa
    17521 agatgctttt agtaaaactg ccactatcaa aaccgaggtg gttttaccgg cggagaccat
    17581 cgctgcgacc gttattatta ataatcgaga gctggaacta accaagtctt cggcggaaga
    17641 ttattggtca ggtgtggctc taattagttc cgacgaggaa aaagagattt tttcaccatt
    17701 agtgccggcg actttattga ctgaagataa tttgggccaa caaaatattt atcaattaga
    17761 ttggaatgag atcaagccaa tcaaaacttc aatttgggaa aaatactttc tttttaagag
    17821 aaacccaacc aataatatgc tgccgatttt aattttcggt aatttttatt tcaaaattct
    17881 ttttgtggtt ttttctttag ccgctattct aaatattgtt gttaaaataa agaagcaaaa
    17941 attagaactt attcttggca gttttggcat tattgtttta ttagctattt ttattttatt
    18001 ttaagcttgt tattttgcaa gtatttgaca aataagtcaa aagataatag tattaaaata
    18061 ctttatatta atttattaaa attgttaaaa aaataattta aattatttta tgaaaagaat
    18121 tgctagtaat ttcaagaggg gaacattttt atgtagtgca atctgtttat tagccaattt
    18181 tgctttattg gcgctgattg ttccgcatag tttagtgaga gctgcttcca tcgatgccgt
    18241 ttcactaaaa ggtattgagg aggcaccatt ttttaaagac aatggctttg tcgtcaatac
    18301 taagccagct ccagatttcg gaggaaagta ttggctagaa aaggataagt caataccaaa
    18361 ctatacgatt gatttagcca agggcgctgc tactaattta agcgatggtt catatcccat
    18421 catgctagtt aaggccccaa ccgaagattt ggccaagtat tatgaacaag aatttggctt
    18481 gagcgatgaa tatattaagg gggttataga tggtcataat ccttttgcct ttattaatat
    18541 ttccaaggga cgggctagat taattcgtaa tgaaaaaatt gatagcaagt ctttagacaa
    18601 caacaataat caaggtcaaa tagatgatca tttagcattg acagttagtt ataagtttcc
    18661 agttggtgaa tattcgtttt tattaacaaa taaaaatagt caattagcta agtggcagtt
    18721 tagagtggtg gataattatc cagccgcgga tatttttgct gttgaaactg cctataatag
    18781 tttaaccttg ggaagcaaca agatatacga gaatattaac ttaccaacaa catataagga
    18841 tacagagatt aagtggacat cttccgatac taacgtgatg accaatggtg gtattattaa
    18901 ccgatcagat actgaagatt tacctgttac tttgacagcc cgcatttctt ccggttcatt
    18961 ctttatggac aaaacatttg acttgatcgt tgtcaagaat gatccctatt tagagattaa
    19021 caatttcact gctaatggaa agtcgatgat taataaagaa aatagtcttt ttattttcca
    19081 aaagcaagac ggaattaaga aatatattat tgattttgct agtggctata aaactaatct
    19141 tcagcaaggc aagtatgccg tcagatttga caatctctct gaagagtcgc aggataagtt
    19201 aataaagtat tattctaagg ataatctttc cgacgaagac agtagattaa ttgcccaagg
    19261 taaagaaccg catgcctata ttcaaaagga taaaaacgga caattaagac taattagtag
    19321 tttccaagac tctgctaata gtaaaagtaa accaattatt ggtattgatc agaaaaattc
    19381 tttcttgatt agagaagatt atcccgatgg tctttatgat attgtagccg atcttgatgg
    19441 ttttgatggt caccataaaa ttaaatggca agttgaggtt gctccagtac cagtaattat
    19501 tgaccccgac agcgccttag tacaagctga ttatgatgct ctgacactag caagtccagt
    19561 taccgaagat tttgatttga agactagtgg cattaatggc tctagtatta gttggtcttc
    19621 aaataatagt ggagcgattg ctattaatgg taatattgcc caagtgacca gaccagaatt
    19681 ttcagctgga gatactgtgg ttcagttgac agcgacatta accaaaggat caattacgag
    19741 aaccaaaact tttcccatca cggttcttaa gaatgaacaa gttgttccta ccactggcgg
    19801 cggcggaggc ggaggtattg ctcacagcct aattccccag agcgttagtg ccattaatgt
    19861 cccattaagt ttggttagta gtcaggatgg agttttaacg caaagtttat ctaatggtct
    19921 gcaggctcga ttagtcgttt ctcctaattc ttttgtcggt ggcgttactt ttataattac
    19981 cgaaggaccg gaagataacg ctttaggtgg acgacatttt atcttaactg ctgttgatag
    20041 caatggtaat cctgttagaa attttactaa taacttgaat attacttttt ccggtttgcc
    20101 attacccgat aatattgccg gagtaggagt ttattattat gacgaggcgt tattgtcttg
    20161 ggttttagtg cctggagcca tttttgatca ggctggcgga tcagttaatt tctttgttaa
    20221 tcatttaacc aaatttgctg tctttagtga gcaaccagtg cttcagcaag ttttaggcga
    20281 aacgacttat gccgacggca cattattacg aggttcagat agacggattt ttgtggtggt
    20341 tgatggcaag ttgcagcata ttttaactct taaagaattg gcgcagtatg ccaatcaaga
    20401 aattctagat gttgaagata gtattattgc ctcgtatcca aaggcttcgc ctaaggctgt
    20461 tttaggggtt agaaaatata atttcaatcg tgatttaaaa accggtatga ccggtgacga
    20521 tgtgcgagaa ttacaacgct atttaaacaa taatggttat gttctagcta aaactggtcc
    20581 tggatcagct ggtcaagaaa ccaagaagtt tggtcgcgct accagagagg ctctcattaa
    20641 attccaaaaa gctaacaaaa ttaatccggc agttggttat tttggaacca tcaccagaaa
    20701 ggctgttaat aaataatata gttatttgat cattttgttt agtcgtttat ttttaaaaac
    20761 caccttatat cggtggtttt taaaaattta tcttcgaata agatcagttt gtgcttagcg
    20821 caagattaat aaaaccgaaa tggataaccg gtttttcctg gccgctattt ttagcggcca
    20881 tgttattgat tattcccagt ctaattatcg cttggctatc tatttctcgt caagattatt
    20941 ttcagcaaca cttggaaaac atcagcttta gcgactaccg tttattggct ctttgttttg
    21001 gttgttacct tctggcttgg ttagctttaa ttattattaa gccggccaac aataattatt
    21061 ttttgtggtt aacagcagct attattttta ttatcagctt agtcactgtg cccttattat
    21121 ccagagatgc agccgcctat gcccttaatg ctgatatggt ctattatggc cattttaatc
    21181 cttatattga gccaattaat ctaggacgcc aatcattcca gctaggttcg ttatggtggc
    21241 taaatcaccc atcgccttac gggccgcttt tcttattaat cattgcttta cc
//
LOCUS       DAOPAI010000010        21181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_2625_length_21181_cov_6.370018, whole genome shotgun sequence.
ACCESSION   DAOPAI010000010 DAOPAI010000000
VERSION     DAOPAI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2625_length_21181_cov_6.370018"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /gene="gap"
                     /locus_tag="PKV49_01985"
     CDS             complement(<1..1012)
                     /gene="gap"
                     /locus_tag="PKV49_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPL22413.1"
                     /translation="MLDRKLRVAINGFGRIGRSVFKALLADQRVEVVAINDLTDNKTL
                     AHLLQYDSCYGRYDKTVSVKDQHLIVDGQTYLALAEKDPTQLPWSSLGVDVVIESTGR
                     FTKADEAKAHIIAGAKKVIISAPAKDETTKNIVLSVNENDLLASDQIISMASCTTNCL
                     APVTKVISDKIGIAKAVMSTVHAYTADQNLVDGPHKDLRRARSATMSIVPTTTGAAAA
                     VAKTIPALSGCFDGSAFRVPTPVVSLCDVVFVTKRPVTVEEVNQLLTTAAEQDKFGAL
                     TVTTEPLVSIDFTGNPASSIVDLSLTKVVGGDLLKIVAWYDNEWGYSHRLANLCAYIA
                     ENN"
     gene            complement(1033..1596)
                     /locus_tag="PKV49_01990"
     CDS             complement(1033..1596)
                     /locus_tag="PKV49_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPL22414.1"
                     /translation="MIISFSGWPGAGKSAVAKKLAAELGWPYYSMGQLRRQAAKRRGL
                     TLEEYNQLALTDITTDLEVDEYQAQLGREQDNFIIDGRTSWHFIPHSYKIFLNVEPLV
                     GAQRIFQDLQQSNERNEGDHLDSVEAVMANNYRRVANDRQRYQQYFHIDAYDLANYDE
                     VVDTTNLNLEQVVAVVSQLVHHKLNQE"
     gene            complement(1602..2555)
                     /locus_tag="PKV49_01995"
     CDS             complement(1602..2555)
                     /locus_tag="PKV49_01995"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPL22415.1"
                     /translation="MRLLVIDDNLAGQRLDKLLANRWPEQSRSEWQKRIEAGSVLVNG
                     QIVKAHYQLIIGDKLKIVPLSRSATLTRATKLAASTEAAAKAKLKIIATEPDFLVVNK
                     PAGLLVHDNVWPPRRPTVAAAIAKKYPAILSLGDSAVRPGIVHRLDKDVSGLMVIPRT
                     VAMFAYLKRQFQYRTVKKCYTALVYGVLSKSEGVINFNLGRSSDGRKMAARPANQEGK
                     AALTEYEVIRQWQNYSLLKVVIKTGRTHQIRAHLAAIDHPLVGDDWYGTNKTKLKNKE
                     LNLGRVFLVASELSFLDLQKKRQKFTIRLPKQLNDLLKQIK"
     gene            complement(2560..3681)
                     /locus_tag="PKV49_02000"
     CDS             complement(2560..3681)
                     /locus_tag="PKV49_02000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22416.1"
                     /translation="MKQPLIASLVQIDQTLTVGLFSRWCNGRAVLWSTAGQLSDLWFR
                     QSWPQISFPARAIIARPFFRLGLFLFLPGLYLLFWRRLATLVKQRQIDGLICLSWLDK
                     IVLTQPALWLKLPVVWLRLPEESEVKLIAPLNAVYRHQAARVKLISYIPQVDDGQQLT
                     GGISEQRLLPALDQASVSQQADLFDSLADGQREQVESKYFVVGAVVDLDNNQAVEILL
                     RSLKIVLSVIPSVQLVIIGDGPQRKELQWLAKQLDLANLVWFVGRQEPLDKWFRSLDA
                     LIELSTTTSRDWYRLLQAMAVGLPIIAPKNRLTTDFLLPGQTAMIVEKPEPEMIAQAV
                     IQLQQDKMLAGRLSWHSRQWIAEFGNLERQLIEFTNYLA"
     gene            complement(3678..5120)
                     /locus_tag="PKV49_02005"
     CDS             complement(3678..5120)
                     /locus_tag="PKV49_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPL22417.1"
                     /translation="MSLAKKFAYNSLIQLIGKGLSVVFGLLGVALITRYLGVIGFGRY
                     VAINNFLGIFAILADLGMTMVTAQMINERPADQSKILNNLCGFRLLSASLILAVGLLA
                     GGLIPYYRQLWPILVILAASYFFIAVNQVFVGFFQSELKTDHLMLSEVVGRILWLAGL
                     IITQQYNWGLGGIIMATAISNLIQLIIAWRLAARRIVIKPAYNQQLWREIASRSWPLA
                     ITITLNLLYLRADILFLNWFKGEAAVGLYGAAYKVIDVLTSLPFLIIGLLLPLLARSW
                     AAGQRARFDFLVSGAINVLIVVVCPLIIGGQLLARPIISLVAGSDFTAAGPILALLLM
                     AIAGIFVSCLFNHILIAINRQRVMIKSYFIVAITAVPAYIVLINWLSYWGAAIVTVYS
                     ELLIAGLSAYWARKLVGWRWLWSVTGKVALANLIMAAIVWPLRDWANRGASQLLLIIL
                     LAAVSYGLSLTVLKVIDWRRWQQIIKSGTV"
     gene            5186..5593
                     /locus_tag="PKV49_02010"
     CDS             5186..5593
                     /locus_tag="PKV49_02010"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22418.1"
                     /translation="MSQSTLALRLIGLASIVLLNLSMFFPTPAAAQFNPSQQRGLNKI
                     GATAYATTGQPQDIRLIIGNGLKVILELIGFVLVILMIISGLQYMTAAGNKTKIDAAV
                     NRIKNAFIGLIIILIAYAATVFIIAQLTAAIGS"
     gene            complement(5599..6246)
                     /locus_tag="PKV49_02015"
     CDS             complement(5599..6246)
                     /locus_tag="PKV49_02015"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22419.1"
                     /translation="MAKIFFIILASLLPLSVSAAATVKLDNPITGINSLLGGDVYIGG
                     VIKRILAVIGSLALLMLVYGGLSIILAAGNETKIKQGKSVVAYTAIGLVVIFMSYTAI
                     TFFLKTLGGNLHDEYEDLPIVPETSFSSENSEEGGFIGPLEEQCPAGCMLDKSCFDLN
                     QPVECWDTPGCCLLINNNEEESISPPETEQCPAGCVLDEPCFTQPTECFDANCCI"
     gene            complement(6253..6837)
                     /locus_tag="PKV49_02020"
     CDS             complement(6253..6837)
                     /locus_tag="PKV49_02020"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HPL22420.1"
                     /translation="MFKHSAVIIIVFVGLFITIFGCPKASVQADACLQGTKRCCTLNA
                     ASCSEIGLTSYSAGDSSCQTLWNNNAGKVCCQGARVNTPIYGRCKNDTDTITQTTDEK
                     PPAPEKKLPKFSSLIKVGHEEFIGNIIKGLLGLTGTLTVIMMVYGGLLWMTAGGNEKA
                     VGQAKQIILWTAVGLVLIFSSYTILKFLFNSLAN"
     gene            complement(6980..7051)
                     /locus_tag="PKV49_02025"
     tRNA            complement(6980..7051)
                     /locus_tag="PKV49_02025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7016..7018),aa:Glu,seq:ttc)
     gene            7146..8195
                     /gene="tsaD"
                     /locus_tag="PKV49_02030"
     CDS             7146..8195
                     /gene="tsaD"
                     /locus_tag="PKV49_02030"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPL22421.1"
                     /translation="MKILAIETSCDETAAAVGHLDDDLIVIDSNIVASQIDIHQAFGG
                     VVPEVAAREHVRQILPVIDQALITAGLNRQTAPRQLSAIAITNGPGLSTSLLVGVATA
                     KALALAWRLPLVPVHHIAGHVAANFINRHLAKIKFPALALTVSGGHTALVLMKNPLNY
                     QIIGQTLDDAAGEAFDKAASLLQLGYPGGPKISALAQEFAKLEKSPNIKLPQPMLRRS
                     DYHFSFSGLKTALLYALKNNREWSKQTSEYAYVFEQAVAEALVRKTIKAAIDFQVKTV
                     FLAGGVAANQQLRAILKTNLAKKTSNVALLLPEMMYTTDNAAMIAASGLLNLQANLTA
                     NPDTVLVQPNRSLIN"
     gene            8208..8504
                     /locus_tag="PKV49_02035"
     CDS             8208..8504
                     /locus_tag="PKV49_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22422.1"
                     /translation="MDIKELVTSLKNEDLYTFERRCKEFFYNNYEFSSLDQDNRDFVI
                     DLLKKYRDDLVNFGGIPGYKIDDKLYEIHSDLSKYGLSENDYDNLKKIINHFRR"
     gene            complement(8501..9871)
                     /locus_tag="PKV49_02040"
     CDS             complement(8501..9871)
                     /locus_tag="PKV49_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPL22423.1"
                     /translation="MQTQANVFKAYDLRGVYERDFDEELAYKLGLAYCLWRGQELAAA
                     RQLTVIIGHDMRLSSPVLSQALAQGLVAGGAKVLDIGLCSTPTFYYAVAEQSADGGIM
                     VSASHNPKEYNGFKIVRDRAKPVSETSGLLELRDLVLNQLLTPVSQPGNYQELAGVLD
                     RQIDYDLSLAGNQPIKSFKIVMDPANAMGGPYFEKLLAKLPQISLIHMNWPLDGSFPN
                     HEADPFKPDNIQPLSQMVVGQQADLGIATDGDADRIFFVDNLGQPLEPGVVRAIMAKI
                     FLAERPGSTIAYDIRPGRITLDTIVANGGQPLVTRVGHSLIKAAMLENNAYFAGESSG
                     HFFLNMQHGCYEVPGIVALKLLQALSASGQTVAEFVSPYNKYAHSGEINRRVPDPAAK
                     IQLIADHYIDGQPDFLDGVTITYPDFWFNVRASNTEPLLRLNLEAVNPEIMAAKRDEV
                     LALLAD"
     gene            complement(9874..11019)
                     /locus_tag="PKV49_02045"
     CDS             complement(9874..11019)
                     /locus_tag="PKV49_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22424.1"
                     /translation="MLETRRLVALFIGVIGLILPITSQAFTARSGDDVNFKSGDQLGG
                     VTYAAGRQITVDTPLPGDLICAGNSVFINQPVQGDVICAAENISIRAKVDGTVRLIGS
                     RLDLDNHIGGNAMLVGSDLIIGQQSVIQGESLIAAQFLDSRGHYLQDFHGIASQIKLY
                     GQYDGPVNLTVANGGNSQVLQVNHGAKLAQGLTYRSKSAAQIDSAAEIKGEIVKKDWS
                     EHGRISQQQIIIGWLWSKLLRLFMLLATGLVIISLWPRASRSMAEVANQRPVLLSAHG
                     LLILIAMPLISVLAAMTIIGLPVAIILLLLWAILLYAARLVVGYWLGRRLWPKAQNNL
                     WPFLVGVTILFIATVVPFIGWLISAIATWWGVGAIWQWVRPANKVNK"
     gene            11072..12667
                     /locus_tag="PKV49_02050"
     CDS             11072..12667
                     /locus_tag="PKV49_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HPL22425.1"
                     /translation="MNIKLIKNFFDLANKQAKTKNDLANLKRQLAELSGQPIISNSQL
                     LSIYNQLLAKKQLEPSRLLLNLLKRRAVRTMSGVAPVAVLTKPWPCAGHCRYCPQQEA
                     VPASYLANEPAVMRAKHCHYHPAKQVHYRLRALLANGHQPNKIELIIIGGTWSYLPLK
                     YQYWYITNCFAAANSHGQTKPRGYRQNLTLAQLKKALAVEQKINETAEYRIIGLTLET
                     RPDCITPTELDRYRQFGCTRVEIGVQIIDDAILQINRRTVTVADITKATKLLRDWGFK
                     ITYHFMPALPGSNPDHDIAMYRQLFSDSRFQPDQIKFYPTVVIKGSQLYEDWRAGYYQ
                     PYTDAELTRVIRACKEATPTYVRIIRLIRDIPEESIEAGNKITNLRQIIQQQGVNCQC
                     IRCREAGHKIAELDQLQLVVKQYPAAGGQEYFISWESRDQQTLFGFCRLRLPDRPAAN
                     NHLRGCALIRELHVYGQLQPPGTVGQVQHRGLGQRLLAEAENIASRHGWTKVAVISGI
                     GVRGYYRKFGYRLSHTYLIKAVL"
     gene            12700..13614
                     /locus_tag="PKV49_02055"
     CDS             12700..13614
                     /locus_tag="PKV49_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22426.1"
                     /translation="MFNPEQFLKRQAEKQESRQNFHNEDYLNQLLSMANAANKIDRIQ
                     YRDFVDLYGEEAVKKDISWVKQQKIEHQKKNSPSEIWHKKIADILEAIFHQQIEMNNY
                     FGENVWTIKTCNYDDFYNHIDAILEIRNPDQKSANYSGVALDVTSASHPERLAKKVDR
                     ILEKIKRGELAKVKYFQSEFLNIRGEKSNAPLFVIGCDLKHTEELAKLWYNDKQRALA
                     QHPIQVLLLNQIIQQAQLYRDYAKKANQAKIADIYQQILDEFTAIADERQPTIEQISQ
                     NPANRQFFTDDTVSANLEKIIQNYVGAN"
     gene            13598..14833
                     /gene="glyA"
                     /locus_tag="PKV49_02060"
     CDS             13598..14833
                     /gene="glyA"
                     /locus_tag="PKV49_02060"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPL22427.1"
                     /translation="MLEQIKDQPILELINQESRRQIDELELIASENYASPAVLAALAT
                     PLANKYSEGYSGKRYYGGNHIIDDIENLAIERAKQLFNAEHVNVQPLSGSPANLAAYL
                     AILEPGDTVLGLRLDHGGHLSHGHPINISGRLFKFVQYEVDATTGRLNMDQVRQVAHQ
                     AKPKLIVAGFSAYSREIDWAGFKSIADEVGALTMADISHTAGLIAGRQLSNPTNIVDI
                     VTTTTHKTLRGPRGAIIMSTTKLAKAIDRAVFPGLQGGPHDHVTAAKAIAFGEALQPA
                     FQDYARQVIANAQLLAEQLQAKNYDIVSGGTDNHLLVIDLTKQGLAGRQAEEALQKAG
                     LSVSRSTVPGDQRPPLNPSGIRLGTAAITTRGFGSAEIKQTANWLHQALVNHADDNYL
                     ADIKQQVTQLGRRLPPPSL"
     gene            14840..15688
                     /locus_tag="PKV49_02065"
     CDS             14840..15688
                     /locus_tag="PKV49_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPL22428.1"
                     /translation="MPLQLINGRQLAEQIKDNIAENIYNLGGPRPNLAIILVGQRPDS
                     QLYVKLKQQEAKKVGIDTHFYSCPANISQAELLEIIRFLNKDPLIDGILLQLPLPQHL
                     NTDEAVNAIHPDKDVDGFTAENLNRLQNNQPQAIIPPVFGVVLKLIAEQKLDLTGQLA
                     VILGNSTIFGDHLAQLIKQQGAEVAIYHRLDKKAQASLNQAKLIISALGRPLAVKIKQ
                     PQENYLIIDIGITKKASKVYGDVDLKSINPLINGWATPVPGGVGPLTIAMALNNTFLL
                     YQQHHE"
     gene            15681..17786
                     /locus_tag="PKV49_02070"
     CDS             15681..17786
                     /locus_tag="PKV49_02070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPL22429.1"
                     /translation="MNSNIWSTLLQLHFGFNQLRPGQEQAIQQVLSGGDCLVIMPTGG
                     GKSLCFQLPALLLPGVTLVISPLIALMKDQVDRLNTIGIPATFINSSIALTEANNRLQ
                     QALTGAYRLIYVAPERFHSQSFQKIIRQLTVSLVAVDEAHCISQWGHDFRPSYLQLQQ
                     FIKNLGRPPILALTATATPEVRQDIVQQLDMVKPQEIITGFGRPNLHFGIISTADGYK
                     KDVVLETIQKAPEASGIVYVSTRQRADELVNFLIDYHISAVEYHAGLTADERQRLQNE
                     FMTGRAKVIVATNAFGMGIDKADLRFVIHYDMPGTIEAYYQEAGRAGRDGQSSLCLLL
                     HSPRDRYLQEFFIEGDNPSVDLILDIYDFLTTFPDNHILITYGSIKSTLGLDIPDLAV
                     GTALKVLEKEGYLAKTYEKTSQATLKLAGPDINLTAGQEALGKRAKKSLEIWGKLYDN
                     YSRKLRAGYSVNLEFLASDLRCSKDSLTRLLKKLQEKEIIIYEPPFKGTEITLLKRCS
                     RQEVQLDRQALRDKLLFARNKLDKMEDYVYHNGCRQKYILNYFGEKTAEDCGHCDWCR
                     HRTTNQRPITKQSSRSRIAKQPATKNIHLETKLTQLTTLELHESGLEINQIASNRNLQ
                     LDTIVEHFCYLLEKNLLKDTSRLLDDNTLSIISDLLKQRPQLINSRLSVIKKELPSYI
                     DWPELKMALAVLKHQLTKQ"
     gene            17783..18496
                     /locus_tag="PKV49_02075"
     CDS             17783..18496
                     /locus_tag="PKV49_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPL22430.1"
                     /translation="MKPARIEQLRQIVADNYQEIAEQFSATRQKPLWPRLRQILRQLP
                     ISGRLLDIGCGNGRLLEELKKRPIDCYVGLDNSTELLKIAAQRYPPTAWPFSISWQPG
                     DLLDTPTGQPFDVVCCIAALHHLPGRANRQLAAKNLCRWLKPTGYLIISVWDLWRQPK
                     YWPKLLAGLWLSLNGQLDYGDLLLNWGQQKSPGRQRYYHAFTKGELKKLIAATGLDII
                     DCQRDGLNLYLVARRPIKN"
     gene            complement(18486..20378)
                     /locus_tag="PKV49_02080"
     CDS             complement(18486..20378)
                     /locus_tag="PKV49_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HPL22431.1"
                     /translation="MSVSRRLFWLVIIGCLLIIVIDGCRLTRAAWLTYQSGLAGKQSL
                     EQGWQLAKAGEWLPAQTAVQQAQQSFLAARDHSQILAKHWLLSRIGWYHQQTNSVYNL
                     LKSAELACQTAVDVLAQAEQWQSNWPSGLTAASLENLTPSSTTQILSQMQGIQPLLLK
                     TLANLHELESSLNQVNFAAGLTKLNAPVGELRQALQDGKALLAEHLPWLQLAPYLAGQ
                     VSDGRFLLILQNNDELRPTGGFIGNYGLLELHNGQVVNLEAHDSYHLDMPAQDYFKPA
                     PPAELNKYLGVKQWFLRDANWSPDFPTTARQVRYFYQEQTKAINKTATAERIDGVIAI
                     TPAIVEDLLGLVGPITVEDQTYNQQNFVELLQYRVEMSYDNYGVTSWNRKAVINDVIR
                     ELEKRLLALPASRWTEVARIVTKNLERKNILLYSDNQAVQQWLVQQNWAGQARSADGD
                     YLWVIDANLAAFKTDAVVDKQINYRLYQSDGQWLAELRLIYKHNGGFDWRTTRYQTYT
                     RVYAPLGSQLISQAGYKDAATTTQELGKTVFGGFIVVEPKKTKEIKLVYRLSDSVVKL
                     LEQNRYELLLQKQPGRQRTSLSLELNLPRSVQRAYSEQLPLNIKNSQVSGQSGWLEDS
                     QVTVNF"
     gene            complement(20479..20976)
                     /gene="rpsP"
                     /locus_tag="PKV49_02085"
     CDS             complement(20479..20976)
                     /gene="rpsP"
                     /locus_tag="PKV49_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPL22432.1"
                     /translation="MLKIKLARIGKKKAPFYRFIISEVGRDPYGRILENLGSYNPKSK
                     ELQAKAERINYWLSQGAQMTTTVNNLLVSHQIIKGEIIRVVSKQPAPVVAETVKPDKT
                     PTAAAAEPVAAEEVAAESTVEEPAAEVAAESTEEPVEEPVSTESAEAVKASDEAGTDA
                     TPNAE"
BASE COUNT         6504 a         5044 c         4116 g         5517 t
ORIGIN      
        1 aattattctc agcaatatag gcacagagat tagctaaacg atggctataa ccccattcat
       61 tatcatacca agccactatt ttgagcagat cgccgccaac caccttggtc aaagataaat
      121 caacaattga gctggctgga ttaccagtaa aatcaatgga aactaacggc tcggtggtaa
      181 ctgtcaaagc gccgaattta tcttgttccg cggctgtggt taataattga ttaacttctt
      241 caacggttac tggccgcttg gtaacaaaca caacatcaca gagcgagaca accggtgtcg
      301 gcacgcgaaa agccgagcca tcaaagcaac cgctcagggc cggaatagtt ttggctacag
      361 ctgcggctgc cccggtcgta gtcggcacga tactcatagt ggcgctacgg gcgcggcgca
      421 aatctttatg cggaccatcc actagatttt gatcggcagt ataggcatga acggtgctca
      481 ttacggcttt agcaatgcca attttgtctg aaataacttt agtcactggc gctaggcaat
      541 tggtcgtaca ggaagccatg gaaataatct gatcactggc taataaatca ttttcattaa
      601 cacttaaaac aatattctta gtggtttcat ctttggctgg ggcgctgata atcaccttct
      661 tggcgccggc gataatatga gccttggctt catcggcctt agtaaaacga ccagttgact
      721 caataactac atcaaccccc aaagaagacc agggcagctg ggtcggatct ttttccgcta
      781 aggccaaata agtctggcca tcaacaatta aatgttggtc tttaacacta actgttttat
      841 catagcgacc ataacagctg tcatactgca acagatgggc taaagtttta ttgtccgtca
      901 aatcattaat ggccaccacc tcaacccgtt ggtcagccag caaagcttta aagacgctgc
      961 ggccaatgcg gccaaaacca ttaatagcca cgcgcaattt acgatctagc atattagtat
     1021 attaaaaaat tattattctt gatttaattt gtggtgaacc aattgactga cgaccgccac
     1081 tacttgttct aaattcaaat tagtggtatc caccacttca tcataattgg ccaagtcata
     1141 agcgtcaata tggaaatatt gttgataacg ctgacgatca ttggctaccc gccgataatt
     1201 attagccata accgcttcaa cactgtctaa atgatcgccc tcattacgct catttgattg
     1261 ttgtaagtct tgaaaaatac gctgagcgcc aaccaacggc tcaacattta agaaaatctt
     1321 ataagaatgg ggaataaaat gccatgatgt tcgaccgtca ataataaaat tatcctgttc
     1381 gcgaccaagt tgggcttgat attcatccac ctccaaatca gtggtaatat cagtcaaggc
     1441 taattgatta tattcttcta aagtaagacc gcgccgttta gctgcctgac ggcgtaattg
     1501 gcccatgcta taatatggcc aacctaattc agccgccaat tttttggcaa cagccgattt
     1561 gccggcaccc ggccagccag aaaaagaaat aatcataaaa tttattttat ttgttttaat
     1621 aaatcattta actgtttggg taggcgaata gtaaattttt gccgtttctt ttgtaaatcc
     1681 aaaaatgaca attcgctagc gactaaaaaa acccgtccca aatttaattc tttatttttt
     1741 agtttagttt tattggtacc ataccaatcg tcgccgacta acggatgatc aatcgcggct
     1801 aaatgagcgc gaatttgatg agtgcgaccg gttttaatta caacttttaa taagctgtag
     1861 ttctgccact gacggatgac ctcatattca gtcagcgctg ccttaccctc ttgattggct
     1921 ggtcgggccg ccattttacg accgtcgctg gaacgaccca aattaaaatt aattacgccc
     1981 tctgatttag ataaaacacc ataaactaag gcagtataac atttcttaac ggttcggtat
     2041 tgaaattgcc gcttgagata agcaaacatc gccaccgtgc gcggaataac catcaacccg
     2101 ctaacatcct tatctaaacg atgaacgatg ccgggacgaa ccgcactatc gcccagcgat
     2161 aatattgccg gatatttctt agctattgct gccgcgacgg tcggtcggcg tggcggccaa
     2221 acattatcat gaaccaataa gccggccggc ttattaacta ctaaaaaatc cggttcagtc
     2281 gcaataatct taagcttggc cttagccgcg gcttcagtcg atgcggctaa tttagtagcc
     2341 cgagtaagcg tggctgaccg cgataacggg acaattttta gcttatcacc gataatcaat
     2401 tgatagtgtg ccttaacaat ttgaccatta actaaaactg atccggcctc aatccgcttt
     2461 tgccattcgg aacgagattg ttcgggccag cgattagcca atagcttgtc caagcgctga
     2521 ccggctaaat tatcgtcaat cactaaaaga cgcataccgt taggctagat aattagtaaa
     2581 ttcaatcaat tgacgctcta ggttgccgaa ctcggcaatc cactgacggc tatgccaaga
     2641 caaacgacca gccaacattt tatcttgttg caactgaatg accgcttgag caatcatctc
     2701 tggctccggt ttttccacaa tcatcgctgt ttgccctggt aataaaaaat cagtcgttaa
     2761 ccggttttta ggtgcaataa ttggtaagcc aaccgccatg gcttgcagca atcgatacca
     2821 atcgcgggag gtggtagttg acaactcaat cagcgcatca agactgcgga accatttatc
     2881 tagcggctct tgccggccga caaaccagac taaattagcc aaatctagtt gtttagccag
     2941 ccattgcaat tctttacgct gggggccatc accgataata actagctgca cgctggggat
     3001 aaccgacaag acaattttca gtgaacgcaa taatatctca accgcctgat tattatccaa
     3061 gtcaacgacc gcgccaacaa caaaatattt agactcaact tgttcgcgct gtccatcagc
     3121 tagactatca aacaggtcgg cctgctgact aacgctagcc tgatccaggg ctggcaacaa
     3181 acgttgttca ctaataccgc cggtcaattg ctgaccgtca tcaacttgag gaatataaga
     3241 aataagcttg actcgcgcag cttgatggcg ataaacagcg ttgagcggag cgattaattt
     3301 tacttccgac tcctctggta accgcagcca aaccaccggc aatttcaacc acaaagccgg
     3361 ctgagtcaac actattttat ccagccaaga caagcaaatc agaccatcaa tctgccgctg
     3421 ttttaccagt gtagctagcc gacgccaaaa taataaatat aaccctggca aaaacaaaaa
     3481 tagccctaac ctgaaaaatg gccgagcaat aatcgctcta gctggaaaac taatctgcgg
     3541 ccatgattgc cgaaaccata aatcgcttaa ttgaccggct gttgaccata aaacggcccg
     3601 accgttacac caacggctga aaagaccgac cgttaaggtc tgatcgatct gaactaaact
     3661 agcaattaac ggttgcttca tacagtacca gatttaataa tctgttgcca acgccgccaa
     3721 tcaataactt taaggacggt taagctcaaa ccataactaa ccgcggccaa taaaattatt
     3781 aacaataatt ggctcgctcc acgattagcc caatcacgca atggccaaac aatcgccgcc
     3841 attattagat tagctaacgc tactttccca gttaccgacc acaaccagcg ccagccaact
     3901 aatttacgcg cccaataggc cgacagaccg gcaatcaaca attcactata gaccgtcaca
     3961 atcgctgccc cccaatatga cagccaatta attaagacta tataagccgg caccgcggta
     4021 atagccacga taaaatatga cttaatcatt actcgctgac gattgatggc aattaagata
     4081 tgattaaaaa gacagctgac aaaaatacca gcaatagcca ttaataataa agccaaaatc
     4141 ggcccagccg ctgtaaaatc tgacccggcc accaagctga ttatcggacg agctaaaagt
     4201 tgaccgccaa taatcaaagg acaaacaaca actattaaaa cattgattgc gccgctgact
     4261 agaaaatcaa acctagcgcg ctggccagct gcccaacttc tggctagaag cggcaataat
     4321 aaaccaataa tcaaaaaagg cagactggtt aaaacatcaa ttaccttgta agccgcccca
     4381 tacaggccga cagccgcttc gcctttaaac cagttcaaaa aaagaatatc ggcccgcaaa
     4441 tataatagat ttaaggtaat tgttatcgct agtggccagg aacgcgaggc gatttcccgc
     4501 cacaactgtt gattataggc cggtttgata acaattcgcc gcgctgccaa acgccaagcg
     4561 ataattaatt gaatcaagtt ggagatagcg gtggccataa ttatcccgcc caatccccaa
     4621 ttgtattgct gagtaattat taagcccgcc aaccataaaa tccgaccgac aacttcggac
     4681 agcattaaat gatcggtctt gagttcgctt tgaaaaaaac cgacaaaaac ctgattaaca
     4741 gcaataaaaa aataagatgc cgctaaaata accagaatcg gccacaattg acgatagtat
     4801 ggtattaggc caccagccag caagccaaca gccaaaatta aactagccga taacaatcga
     4861 aagccgcaca gattattcaa aattttcgac tgatcggctg gtcgttcatt gatcatttga
     4921 gctgttacca tagtcatacc caaatcagcc aaaatagcga aaatacctaa aaaattatta
     4981 atagcaacat agcggccaaa accaataact cccaaataac gagtgattaa agccactccg
     5041 agcaaaccaa aaacaaccga cagacccttg ccgattaatt gaattagact attataagca
     5101 aacttcttag ctaaagacat ccccgttgtt ttttaagtga ccatcagata taataactat
     5161 atcacaatat cttaaaaaac caactatgtc tcaatcaact ctggctctcc ggctaatcgg
     5221 tttggccagt atcgtacttc ttaatttatc aatgtttttc ccaacaccgg cggcggccca
     5281 atttaatccc agccaacaaa gaggattaaa taaaatcggt gcaacagctt atgctacaac
     5341 cgggcaaccc caggatattc gcttgattat cggcaatggc ctcaaggtaa ttctagaatt
     5401 aatcggtttt gtcttagtca tcttaatgat aatttccgga ttacaatata tgacggccgc
     5461 cggtaataaa accaagattg acgccgctgt caatcgtatt aagaatgctt ttatcggatt
     5521 gataataatt ctaattgcct acgcggccac tgtttttatc atcgcccaat taacagccgc
     5581 tatcggatct taatttaatt aaatacagca attggcatca aagcattcgg ttggttgagt
     5641 gaaacaaggc tcgtctaaca cacacccggc tggacattgt tcagtctcag gcggactgat
     5701 agattcttct tcattattat taataagtag acaacaacca ggagtatccc aacattcgac
     5761 tggctggttc aagtcaaaac aagatttatc aagcatacat ccagctggac attgttcctc
     5821 aaggggaccg ataaatcctc cttcttcact attttcacta ctgaaagagg tctccggtac
     5881 aatcggtaga tcctcatatt catcgtgcag attgccaccc aatgtcttca agaaaaaggt
     5941 aatcgcggta taagacataa aaataaccac caaaccaata gctgtatagg ctacaacact
     6001 tttgccttgt ttaattttag tttcattgcc ggcagccaaa ataatggaca gaccgccgta
     6061 aaccagcatt aataaagcca gcgaaccgat cacagccaaa attcgcttaa taactccgcc
     6121 tatatagaca tcgccaccca ataaactatt gataccggtt ataggattat ctaatttaac
     6181 ggtggcagca gccgagactg acagtggcag taggctagct aaaatgataa aaaatatttt
     6241 tgccatagac gattaattag ccaaactatt aaataaaaat ttcagaatgg tgtacgaaga
     6301 aaatatgaga actaaaccga cagctgtcca caaaattatt tgtttagctt gaccaaccgc
     6361 cttttcgtta ccgccggctg tcatccacaa taatccgcca taaaccatca taataacagt
     6421 caaagtcccg gttaagccca ataatccttt aataatattg ccaataaact cttcatgccc
     6481 gactttaatc aggctggaaa attttggcag ttttttctcc ggagctggcg gtttttcatc
     6541 agtggtttgc gtaatggtat ctgtgtcatt cttacacctt ccatatattg gtgtattcac
     6601 cctagcgcct tggcaacaaa ctttgccagc attgttgttc catagtgtct ggcaagatga
     6661 atcgccggcc gaatatgaag ttaaaccaat ttcactacaa cttgcagcgt tcaaggtaca
     6721 acaacgtttt gttccttgta aacaagcatc agcctgcaca gaagccttcg ggcaaccaaa
     6781 aatagtaata aataaaccaa caaatactat tataataacg gcgctatgtt taaacataga
     6841 aaaaataaat atttaattat ttaattataa caaaaaatca attgaccggc tactatttat
     6901 tttaaaatgt atcttgatta taaaaacatg cttaataaaa ctcattcaaa aaaacaggcc
     6961 atttagcctg ttctttatgt agtccctagg ggaatcgaac ccctgttatc aggatgaaaa
     7021 cctggtgtcc taaccactag acgaagggac cgtataactt tgtcattata actaaacttg
     7081 ctgggctgtc aagctgtggc cagacttgaa taatcgccaa accgctgata caataagctt
     7141 aacttatgaa aattttagcc attgaaacta gttgcgacga aaccgcagcc gctgtcggtc
     7201 acctggatga tgatttaatt gtcattgaca gcaatattgt cgcctcgcag attgatatcc
     7261 accaagcctt cggcggagta gttccggagg tggcggcccg cgaacatgtt cggcaaattt
     7321 tgccagttat tgatcaggcc ttgatcacgg ccggattgaa tcgccaaact gcgccgcgcc
     7381 agttatcagc tattgccatt actaacggtc cgggtttatc aacttcactg ttagttgggg
     7441 tagcaacagc caaagcattg gctttggctt ggcgactgcc gctagtaccc gttcatcata
     7501 tcgccggcca tgtcgccgcc aatttcatca atcgccatct ggctaaaatt aaattcccag
     7561 ctctggcctt aacggtcagc ggcggccaca cagccctagt tttaatgaaa aatccgctta
     7621 attatcagat tattggccaa actctagacg atgccgctgg tgaagctttt gataaagcag
     7681 ccagcttgct gcagttgggt tatccgggcg gtccaaaaat aagcgcgcta gctcaagaat
     7741 ttgctaaatt agaaaaatca ccaaacatta aactgcctca accaatgctg cgacggtctg
     7801 attatcattt ttctttttcc ggcctcaaaa ccgccctact ttatgccctg aaaaataacc
     7861 gcgagtggtc aaaacaaacc agcgaatatg cctatgtttt tgagcaagcg gtggccgaag
     7921 cgctcgttcg caaaacgatt aaagccgcga ttgattttca agttaaaact gtttttctgg
     7981 ctggcggcgt tgccgctaat caacagttgc gcgccatatt aaaaacaaac ttagctaaaa
     8041 aaacatcaaa tgtggcacta ttattaccgg aaatgatgta taccaccgat aacgccgcca
     8101 tgatagccgc tagcggttta cttaatttac aggccaacct aacagccaac cccgacacgg
     8161 tcttagttca accaaatcga tcactaatta attaataatc tacgcctatg gacatcaaag
     8221 aattggtaac tagccttaaa aatgaagatt tatacacttt tgagcgtcgc tgtaaagaat
     8281 tcttctataa caattacgaa ttctctagtc ttgaccaaga caatcgcgat tttgttattg
     8341 atttattaaa aaaataccgc gacgatttgg ttaatttcgg cggcattccc ggctataaga
     8401 tagatgacaa gctgtatgaa attcacagcg acttatctaa atatggactc agtgaaaacg
     8461 attatgataa tttaaaaaaa atcatcaatc acttccgccg ctagtcagcc aataacgcta
     8521 aaacttcgtc gcgcttagcc gccataattt ctggattaac cgcttccaaa ttaagacgta
     8581 ataaaggttc ggtattagac gcccgaacat taaaccagaa atctggataa gtaatggtaa
     8641 cgccatccaa aaaatcaggc tggccgtcaa tataatggtc ggcaattaat tgtattttgg
     8701 ccgctggatc aggaactcga cgattaatct cgccggaatg agcgtatttg ttgtaaggac
     8761 tcacaaattc agccacagtc tgaccgctgg ccgacaaagc ctgcaacagc tttaaggcta
     8821 caattcctgg cacttcataa caaccatgct gcatatttaa gaaaaaatgg cccgatgatt
     8881 caccagcaaa ataagcatta ttctccagca tagctgcttt tatcaatgaa tggccgaccc
     8941 tagtcactaa cggctgaccg ccgttagcca caatcgtatc caaagtaatc cgaccgggcc
     9001 gaatatcata ggcaatagtg gaacctggcc gttcagctaa aaaaatttta gccataatgg
     9061 ctcggaccac cccgggttct agcggctggc caaggttgtc aacaaaaaat atccgatcag
     9121 catcgccatc ggtggcgata cctaaatcgg cttgctgacc gacaaccatt tgactgagcg
     9181 gttggatatt atccggttta aatggatcgg cctcatggtt aggaaacgat ccatccagcg
     9241 gccaattcat gtgaattaat gagatctgcg gtaatttagc taataatttt tcaaaatagg
     9301 gaccgcccat ggcattggct ggatccatca caatcttgaa tgatttaata ggctgattac
     9361 ccgctaatga caggtcataa tcaatttgcc tatccaaaac accggctagc tcctggtaat
     9421 tacctggctg gctaactgga gttaacaact gattcaatac caagtcgcgc agttccaata
     9481 acccgctagt ttcactgact ggtttggctc gatcacgaac aatcttgaaa ccattatatt
     9541 ctttagggtt atgcgaagcg ctcaccataa taccgccatc ggcggattgt tcagccacgg
     9601 cataataaaa ggtcggcgta gaacaaagac caatatctaa aactttagcc ccgccggcaa
     9661 ctaaaccctg agccaaggct tgactcaaaa ccggcgacga taaacgcata tcatgaccaa
     9721 taataacggt caattgacgg gcagcagcca attcctgccc acgccacagg caataagcta
     9781 atcctaattt ataagctaat tcttcatcaa aatcgcgttc ataaacgccg cgtaaatcat
     9841 aggccttaaa aacattagct tgtgtctgca taattattta ttaactttat tggctggtct
     9901 gacccattgc caaattgctc ccacacccca ccaagtagca atggcgctaa ttaaccagcc
     9961 gatgaatggc acaacggtgg caataaatag gatggtgacg ccaaccaaaa atggccagag
    10021 attattttgc gctttcggcc ataaacgccg acccaaccaa tagccgacaa ccaatctagc
    10081 ggcatatagc aaaatagccc ataacaataa taatataatc gccaccggca aaccgataat
    10141 ggtcatagcg gccagaacgg atattagcgg catcgcgatt aatatcaaca aaccatgcgc
    10201 cgacaataaa accggtcgct gattggctac ttcagccata gaccggctag ctcgtggcca
    10261 taaactaatg attactaaac cggtagcaag tagcataaat aagcgcagta atttagacca
    10321 caaccaaccg attattatct gttgttgact aatccggcca tgctccgacc aatccttttt
    10381 cacgatttcg cctttgattt cggccgctga atcaatttgc gccgctgact tggaacgata
    10441 agtcaaacct tgagccagct tagccccatg attaacctgt aaaacctggc tattcccacc
    10501 gttagctaca gttaaattaa ctggtccatc atattgacca taaagtttga tttgactggc
    10561 gataccatga aaatcctgta aataatgacc gcgactatcc aaaaactgcg ctgcaattag
    10621 tgattcgccc tgaataacgg attgctgacc aataattaaa tcggaaccaa ccagcatagc
    10681 attaccgccg atatgattat ccagatcgag acgactgcca atcaagcgaa ctgttccatc
    10741 aaccttagcc ctgatactga tattttctgc cgcgcagata acatcgccct gaaccggctg
    10801 attaataaaa acgctattac cggcacaaat caaatcaccc ggcaatggcg tgtcaacagt
    10861 aatctgtcga ccggcagcat aggtgacgcc gcccaattga tcgccagact taaaattgac
    10921 atcatcaccg ctgcgggcgg taaaagcctg gctagttata ggcagaatta aaccaattac
    10981 accaataaat aaagctacta accgccttgt ctcaagcata taattgtgat taaattaaaa
    11041 agctatcagt taaattataa catcagtgac aatgaatatc aaactgatta aaaatttttt
    11101 cgatttagcc aataaacaag ccaaaaccaa aaatgattta gctaatttaa aacgtcaact
    11161 ggcggaatta tccggccagc caattattag caatagccaa ttattatcta tctataatca
    11221 attattagct aaaaaacagc ttgaacccag ccgattatta ttaaatttat taaaacgccg
    11281 tgccgttcgt actatgtccg gcgtggcgcc ggtggctgtc ttgaccaagc cctggccctg
    11341 tgccggccat tgtcggtact gcccgcagca agaagctgta ccggccagct acctagccaa
    11401 tgaaccagcg gtaatgcgcg ccaaacattg tcactatcat ccagccaaac aagtccacta
    11461 tcgcctgcgc gctctgctgg ctaatggtca ccagccaaat aaaattgaat tgattattat
    11521 cggcggcact tggtcctatc tgccgcttaa gtatcaatat tggtatatta ctaattgttt
    11581 tgccgccgcc aatagccatg gtcaaacaaa gccacgcggc taccgacaga atttaacatt
    11641 agctcaatta aaaaaggctc tggcggttga acaaaaaatc aacgaaaccg ctgaataccg
    11701 aattattggt ttgacactag aaactagacc agactgtata acaccgactg agcttgaccg
    11761 ctaccgccaa ttcggctgta ccagagtaga aatcggcgtc caaataattg atgatgccat
    11821 tttacaaata aatcggcgaa ccgtcacggt ggctgatatt actaaagcta cgaaattatt
    11881 acgcgattgg ggttttaaaa ttacttatca ttttatgcca gccttgcctg gatctaaccc
    11941 tgatcacgac atcgccatgt atcggcaatt atttagcgat tctcgttttc aacccgacca
    12001 aatcaaattt tatccaaccg tagttatcaa gggtagccag ctgtatgaag actggcgagc
    12061 tggttattat cagccataca ccgatgccga gttaactcga gtcattcgtg cctgcaaaga
    12121 agcgacgcca acctatgtcc gtattattcg cttaatccgt gatatccctg aggaatcaat
    12181 tgaagctggc aataaaatca ctaatttacg ccaaattatt caacaacagg gcgtcaattg
    12241 ccaatgcatc cgctgtcgtg aagctggcca taagatagcc gaactagacc agctacaact
    12301 ggtggttaaa caatatccag ccgcgggcgg tcaagaatat tttattagct gggaaagtcg
    12361 cgatcagcaa actttgtttg gtttttgccg attacggtta ccagaccgac cggcagcgaa
    12421 taatcacctg cgcggttgcg ctctaattcg cgaactccat gtttatggcc aattacaacc
    12481 acccggcaca gttggtcaag tccagcatcg cggccttggt cagcgcctat tagcggaagc
    12541 tgagaatatt gctagtcggc acggctggac taaagtggca gttatttctg gaatcggtgt
    12601 ccgcggctat tatcgcaaat tcggctatcg cctcagccat acttatttaa ttaaagctgt
    12661 gctatagtaa ttataaacat taatgattaa tgtaaaacta tgttcaatcc agaacaattt
    12721 ttaaaacggc aagctgaaaa gcaagagagc cgacaaaatt ttcataatga ggattattta
    12781 aatcagctct tatcaatggc taatgcagcc aataaaattg accgcatcca ataccgcgat
    12841 ttcgttgatt tgtacggcga agaagctgtt aaaaaagata ttagttgggt taaacaacaa
    12901 aaaattgaac atcagaaaaa aaattctccc agtgaaatct ggcataaaaa aattgccgat
    12961 attctggaag ctatttttca tcaacagatt gaaatgaata attatttcgg tgaaaatgtt
    13021 tggaccatta aaacttgcaa ctatgacgat ttctataatc atattgatgc tattttagaa
    13081 atacgtaatc ccgatcaaaa atcagctaat tactccggtg tcgcgctaga tgttacctcg
    13141 gctagccacc ccgaacgcct ggctaaaaaa gtcgatcgta tcctggaaaa aattaaacgc
    13201 ggtgagctag ctaaagttaa atacttccaa tcagaatttt taaatatccg cggcgaaaaa
    13261 agcaatgcgc ctttatttgt tatcggctgc gatctaaagc ataccgaaga actagctaaa
    13321 ctttggtata acgataaaca acgcgcctta gctcaacatc ctattcaagt tcttttgtta
    13381 aaccaaatta ttcaacaggc ccagctttat cgcgattatg ccaaaaaagc caaccaagca
    13441 aaaatagccg atatctatca acaaatttta gacgaattta ccgctattgc tgacgaacga
    13501 caaccaacaa ttgaacaaat cagccaaaac ccagccaacc gccaattttt taccgatgat
    13561 actgttagcg ctaatctgga aaaaattatt caaaactatg ttggagcaaa ttaaggacca
    13621 gccgatactg gaattaatta atcaagaaag ccgccgccaa attgatgaac tagaattaat
    13681 cgcctcagaa aattatgctt cgccggcagt tctagcggcg ctagccaccc cgctagctaa
    13741 taaatacagc gagggttaca gtggtaaacg ttattatggc ggcaaccaca tcattgatga
    13801 catagaaaac ctagccattg aacgagctaa acagcttttt aacgctgagc atgttaatgt
    13861 tcaaccacta tccggctcgc cagctaatct ggccgcttat ctggctattt tagagcctgg
    13921 tgacacggtg ttaggcttgc gtctagatca cggcggccat ttatctcacg gacatccgat
    13981 taatatttcc ggccgtttat ttaaattcgt ccaatacgag gtcgacgcca ccaccggtcg
    14041 gttaaatatg gaccaagtcc gtcaagtcgc tcaccaagcc aaaccaaaat taatcgtcgc
    14101 cggtttcagc gcttatagtc gagaaattga ttgggctggc ttcaaatcta tcgctgacga
    14161 agttggagcg ttgactatgg ccgatatttc gcacaccgcc ggcttaattg ctggccgaca
    14221 attaagtaat cccaccaata ttgttgatat cgttacgaca accacccata agaccttgcg
    14281 cggtccgcgc ggagcaataa tcatgtccac aaccaagctg gctaaagcta ttgaccgcgc
    14341 tgtctttcct ggtttacaag gtggaccgca cgaccatgtc accgctgcca aagccattgc
    14401 ctttggcgaa gctctgcagc cagccttcca agactatgct cgccaagtta tcgctaatgc
    14461 ccaactgtta gctgaacagc tgcaagccaa gaattatgat attgtttccg gcggcactga
    14521 caaccattta ttagtcattg acctaaccaa gcaaggacta gccggtcgcc aagccgaaga
    14581 agctttgcaa aaagccggct tatccgtttc tcgttccact gttcctggcg atcagcgacc
    14641 gccgctcaat ccgtccggta ttcgtctagg aacagccgct ataaccaccc gtggttttgg
    14701 ttcggcggaa attaaacaga cagccaattg gcttcatcag gccctggtta atcatgccga
    14761 tgataattat ttagccgata tcaaacagca agtcactcaa ttgggtcgac gcctgccgcc
    14821 gccaagtctc taaattatta tgccgttaca attaatcaat ggccgtcaac tggccgaaca
    14881 aatcaaagac aatattgctg aaaatattta taacctaggc ggccctcggc caaacctggc
    14941 gattatttta gttggccaac ggcctgattc tcagctctat gttaagctaa aacaacaaga
    15001 ggcgaagaaa gtcggcattg atactcattt ttatagctgt ccagctaata ttagccaagc
    15061 cgaattattg gaaattatcc gctttttaaa taaagatccg ctgattgatg gcattctatt
    15121 gcaattaccg ctgcctcagc acttaaatac cgacgaggca gtcaacgcta tccatcccga
    15181 taaagatgtt gacggcttta ccgctgaaaa tctaaaccgt ctgcaaaata atcagccaca
    15241 agccattatt ccaccggttt tcggcgtagt tctcaagctg attgccgagc agaaattaga
    15301 tttaaccggt caattagcag taattttggg aaattcaact atttttggag atcatttggc
    15361 ccagctaata aaacagcagg gcgccgaagt tgcaatatat caccggttag ataaaaaagc
    15421 tcaagccagt ttaaaccaag ctaagctaat tatcagcgcc ctgggccgcc cgctagccgt
    15481 taaaattaaa caaccccaag aaaattatct aattattgat attggcatca ctaaaaaagc
    15541 aagtaaagtc tacggtgatg tggacttaaa atcaataaac ccactaatca atggctgggc
    15601 aacgccagtg ccaggcggtg tcggaccgct gacgattgcg atggcgctca acaatacttt
    15661 tttactttat cagcagcatc atgaatagta atatttggtc aactttatta caacttcatt
    15721 ttggttttaa tcaacttcgt cccggtcaag aacaagctat tcagcaagta ttgtcgggcg
    15781 gcgactgctt ggttattatg ccaaccggcg gtggaaaatc actttgcttc cagctgccag
    15841 cgctactatt acccggtgtc actctggtta tttcaccatt aatcgctttg atgaaagatc
    15901 aggttgaccg gttaaatacg atcggcatac cagctacttt cattaatagc tctattgcac
    15961 taactgaagc taataaccgt cttcaacaag ctctgacagg ggcttatcgg ctaatttatg
    16021 tcgctccaga acgttttcat agccaatcat ttcaaaaaat tatccgacaa ttaactgtga
    16081 gtctggtcgc cgtcgatgaa gcgcattgta tcagccaatg gggccatgat ttccggccaa
    16141 gctatttgca gttacagcaa ttcattaaaa acctggggcg gccgccgata ctagcgctaa
    16201 ccgctaccgc tacacctgag gtccgtcaag acattgtcca gcaactggat atggtcaagc
    16261 cgcaagaaat aattaccggt tttggccggc caaacttgca ttttggcatc attagcactg
    16321 ctgatggtta caaaaaagac gtggttctag agactattca aaaagcgcca gaagctagcg
    16381 gtattgttta tgtcagtact cgccagcgcg ctgatgaatt agttaatttt ttaattgatt
    16441 atcacatctc agccgtagag tatcatgccg gcttaacggc tgatgaacgg caacgattgc
    16501 aaaatgaatt tatgaccggc cgggctaaag taattgtcgc cactaacgcc ttcggcatgg
    16561 gcattgataa agccgacctg cgctttgtca tccattatga tatgcctggc accattgagg
    16621 cttattatca agaagctggc cgagctggcc gcgatggtca atccagtctt tgtctgctac
    16681 tccatagtcc gcgtgatcgc tacctgcaag aattttttat tgagggcgat aatccttcag
    16741 ttgatttaat tttggatatt tatgattttt taactacctt tccagacaat catattttaa
    16801 tcacctacgg ttctataaaa agcactcttg gtcttgatat tccggattta gcggttggta
    16861 ctgctcttaa agttctagaa aaagagggtt atctggccaa aacctatgaa aaaaccagtc
    16921 aagcaactct caaactagct ggtccagaca tcaatttaac cgccggacaa gaagctttag
    16981 gtaagcgggc gaaaaaatct ttagaaattt ggggtaaact ctatgataat tactctcgca
    17041 aactccgtgc cggctattcg gttaatctag aattcttagc cagtgatttg cgctgctcta
    17101 aagattcttt gactaggctg ttaaaaaaac tgcaagaaaa agaaataatt atttatgagc
    17161 cgccgtttaa aggaaccgag ataactctat taaaacgctg ttcccgccaa gaagtccagc
    17221 tggatcgaca agccttgcgc gataaactgc tttttgcccg caacaaacta gataagatgg
    17281 aggactatgt ctatcataac ggttgccgac aaaaatatat tctcaattat tttggcgaaa
    17341 aaacagctga agattgcggt cactgtgatt ggtgccgcca tcgcaccact aatcaacgac
    17401 cgataaccaa acagtccagc cgatcacgaa tagccaaaca accagcaact aaaaatatcc
    17461 atttagaaac caagctaacc cagttaacca ctctagaact ccatgaatcc ggattagaaa
    17521 tcaatcaaat cgccagtaat cgcaatctgc agctagatac aatcgtggaa catttctgct
    17581 atttactaga aaaaaaccta ctaaaagata ctagccggct gcttgatgac aataccttgt
    17641 caatcatcag cgatttgtta aaacaacggc cgcaactaat taatagtcgt ttaagtgtca
    17701 ttaaaaaaga attgcccagt tatattgatt ggccggagct aaaaatggca ctagctgttc
    17761 taaaacatca attgaccaaa caatgaagcc ggcgcggatt gaacaattaa ggcaaattgt
    17821 cgctgacaat taccaagaaa ttgctgaaca attctcggca acccgtcaaa aaccactttg
    17881 gccgcgattg cgacaaatac ttcgccaatt gccaatcagc ggccgactgc ttgatattgg
    17941 ctgcggcaat ggacgcttgt tagaagaatt aaaaaaaaga ccgattgact gttatgtcgg
    18001 cctggataat tctactgaat tgttaaaaat tgctgctcaa cgctatccgc caaccgcttg
    18061 gccattttct attagctggc aaccgggcga tctcttagac acaccgaccg gacaaccatt
    18121 tgatgttgtc tgttgtattg ccgctttaca tcatttaccc ggtcgcgcta atcgccagct
    18181 ggcggccaaa aacttgtgcc gctggctcaa accaactggt tatttaataa tcagcgtttg
    18241 ggatttatgg cgccaaccta aatattggcc aaaattatta gctggcttat ggttatctct
    18301 caatggtcag ctagattacg gcgacttgtt gttaaattgg gggcaacaaa aatcaccggg
    18361 acgacaacgc tattatcatg cctttaccaa aggcgaatta aaaaaattaa tagcggcaac
    18421 cggccttgat attattgatt gtcaacggga tggacttaat ctctatctag tcgcccgccg
    18481 gccaattaaa aattgactgt cacttggcta tcctccaacc agcccgattg accactgacc
    18541 tggctatttt ttatattcaa gggtaattgt tcagaataag cccgctgaac tgatcggggc
    18601 aagtttaatt ctaacgacaa cgatgtccgc tgacgacctg gctgtttttg taaaagtaat
    18661 tcatagcgat tctgctctaa aagcttaacc actgaatcgg acaaacgata aaccaactta
    18721 atttccttgg tcttttttgg ctcgacgacg ataaagccgc caaaaactgt cttccccaat
    18781 tcttgagtag tggtagccgc atctttataa ccagcttgac taatcagctg gctgccaagt
    18841 ggcgcataaa cccgagtata agtctgatag cgagttgtcc gccaatcaaa accgccatta
    18901 tgtttataaa tcagccgcaa ttccgccaac cattggccgt cggattggta aagccggtaa
    18961 ttaatctgct tatcaaccac ggcatcagtt ttaaaagccg ccaaattagc gtcaattacc
    19021 cataaataat caccatcagc agaccgagct tgaccagccc aattttgttg gactaaccat
    19081 tgttggacag cttggttgtc actgtataat aaaatattct tccgttctaa gtttttagtg
    19141 actattctcg ccacttcggt ccagcggctg gccggcaaag ccaataaccg cttttccaat
    19201 tcacgaatta catcattaat aactgcctta cgattccaag aagtaacacc ataattatca
    19261 taagacattt ccacccgata ttgtaataac tccacaaaat tctgctgatt ataggtctga
    19321 tcctcaaccg taatcggccc gaccagacct aataaatctt caacaatagc cggagtaatg
    19381 gcaatcacgc catcaatgcg ttcagccgtg gcggtcttat taatcgcttt ggtttgctct
    19441 tgataaaaat aacgcacttg ccgagccgtc gtcggaaaat ctggcgacca attggcatcg
    19501 cgcagaaacc attgtttgac gcctaaatat ttattcaatt cggctggcgg cgctggttta
    19561 aaataatctt gcgccggcat atctaggtga tagctatcat gagcctctaa attaacgact
    19621 tggccattgt gcaattctaa taaaccataa ttaccgataa aaccaccggt tggccgtaat
    19681 tcatcattat tctgcaaaat taatagaaaa cgcccatcgc taacttggcc agccaagtat
    19741 ggcgctagct gcaaccatgg caaatgttcg gccaataagg ctttgccgtc ttgcagtgcc
    19801 tggcgcaatt caccgactgg agcattaagc ttagtcaagc cagcggcaaa attaacttga
    19861 ttcaagctac tctcgagttc atgcaggtta gctaaagttt ttaacaacag cggctgaata
    19921 ccctgcatct gcgacaatat ttgagttgta ctgctaggtg ttaaattctc caatgaagcc
    19981 gccgtcaaac ccgacggcca attggattgc cattgttcgg cttgagccaa cacatcaaca
    20041 gcagtctgac aagctaattc agctgatttc aataaattat aaacactatt ggtctgctga
    20101 tgataccaac cgattctaga taataaccaa tgcttggcca gtatttgact atgatcgcgc
    20161 gccgctaaaa aagattgttg cgcttgttgc acagccgttt gcgctggtag ccattcacca
    20221 gctttagcta attgccagcc ctgctctaat gattgtttgc cggctaaacc cgattgatag
    20281 gttaaccaag ccgcccgagt taaacgacaa ccatcaataa caataatcaa tagacaacca
    20341 ataattacta accaaaataa acggcggctt acggacataa cattaaatat tactaattat
    20401 tttataatta tataacaaaa ttgagcagag ccaaaataaa aaacagagcc gcctagctct
    20461 gtttttcaat aatcaattct attcggcgtt tggcgtggcg tcagtcccgg cctcgtcgct
    20521 agctttaacc gcttcagccg actctgtgga gactggctct tcaactggtt cttcggttga
    20581 ctcggcagca acctcggcag ccggctcttc aacagttgac tcggcagcaa cctcttctgc
    20641 ggcgaccggt tcagcagcag ccgcagttgg cgtcttgtct ggcttaacag tttcagcgac
    20701 aactggagcg ggctgcttac tgacaacacg aataatttcg cctttaataa tctgatggct
    20761 aactagtaaa ttattaacag tagtggtcat ctgagctccc tggctcagcc aataattaat
    20821 tcgttcagcc ttggcctgta attctttaga cttgggatta taggagccga gattttccag
    20881 gatccgtcca tagggatcac ggccgacctc agaaataata aaacgataga atggcgcttt
    20941 ctttttgcca atacgggcaa gcttgatttt cagcataaac aatgcctgtt tagataataa
    21001 ctaaataagt gagcattaac ggtagcttga ttatagcctt aattagcaat ttttgtcaag
    21061 aaaatggggg gtatagcaaa actggctaat aaataaacaa aaccactatc agtaagtggt
    21121 tgtgcagagc gggtaacggg aatcgaaccc gtatcttcag cttggaaggc tgacataaaa
    21181 g
//
LOCUS       DAOPAI010000011        20961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_2655_length_20961_cov_5.309911, whole genome shotgun sequence.
ACCESSION   DAOPAI010000011 DAOPAI010000000
VERSION     DAOPAI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2655_length_20961_cov_5.309911"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKV49_02090"
     CDS             complement(<1..743)
                     /locus_tag="PKV49_02090"
                     /inference="COORDINATES: protein motif:HMM:NF018155.2"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPL22433.1"
                     /translation="MTDKKKVVLIIRDGWGYNPEVEGNYIRQAQTPNENKYLSTYPHS
                     LLQASGEAVGLEDGYQGNSEVGHMTLGAGRIIFQSLVRINKAIEDGSFFKDQAFLSVI
                     KNCQTQNKKLHLIGLLQTQGVHAHLNHLLALLDLCHKENFHNVLIHVITDGRDAPITE
                     SLIHVATVQDKLKELGFGQIATISGRYYAMDRDQRWDRTEKAYQAIVEGQASEFTDPL
                     AIITAKHQQGETDEFIEPCKVKNYSGIAE"
     gene            complement(833..919)
                     /locus_tag="PKV49_02095"
     tRNA            complement(833..919)
                     /locus_tag="PKV49_02095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(883..885),aa:Ser,seq:gga)
     gene            complement(945..1715)
                     /locus_tag="PKV49_02100"
     CDS             complement(945..1715)
                     /locus_tag="PKV49_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPL22434.1"
                     /translation="MFGQVKTKQRKFTDSFLLIVLVVIFCVFVALKIYQSLSKPSVTV
                     NNDEQAEIIKDIQASNQEAWQNVTVELQEVPPIKSDDHLLGQAEAPAQLIVYSDLTDP
                     LAASFLPIVRQLKEQVGDAVVIAWRQHPLANNQLAQEAAAAAECAGQQNKFWEFAEQV
                     AATASQEKPFDDWTAIAQNVGLGLTEFEKCLTKPEVQATIDKQSAEAEALGAIGVPSS
                     FLNKTLLSGVYPLADFVGSDGQTEPGLLKLVQQASRQQ"
     gene            complement(1726..3180)
                     /gene="gatA"
                     /locus_tag="PKV49_02105"
     CDS             complement(1726..3180)
                     /gene="gatA"
                     /locus_tag="PKV49_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPL22435.1"
                     /translation="MDLNNLTLAAARQLLDNRQITSRQLTEYYLRRLKKYDDQVHASL
                     LIDETGALKQADEADQRLISGDQTGVLGIPYLVKDNILTKGLKTTAASKILADYIAPY
                     DATVIAKLRQSGAVLLGKANLDEFAHGTSTENSAMGPTKNPYDLERVPGGSSGGSAAA
                     VAADFCLFALGSDTGGSVRHPASLCGVVGYKPSYGAISRHGLIAMASSTDVIGILAKT
                     ASDAASVLRIIAGQDKFDATAQTKLATAKIKDKINWADLRIGWPDEYVSTGLDLAVLD
                     SLTQVKIFLQEHGAKIIPLSLPSTKYGVAVYHVITSAEVSANLARFDGLRYGYKQSAA
                     SLNETYLINRGHFGAEAKRRIMLGTYILSAGDGCFYYNQAQRVRRWISYDFDQAWEKV
                     DFLLTPTVPRPAFRLSQKINDPLSLYLEDALLTPASLAGVPAVSWPTGWSGDGLPIGS
                     QLIGPLGNDYDLLTLTQAMQTALPCLRRRLAWEK"
     gene            complement(3183..3497)
                     /locus_tag="PKV49_02110"
     CDS             complement(3183..3497)
                     /locus_tag="PKV49_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HPL22436.1"
                     /translation="MKLDKQTISHLANLAKIKLEDGEAEIYSQQLSDIIGHLNNLSEA
                     DKFIQQAQLTDQIDGYQFKAASVDLSADEVATWPADETAASLAMTGSQPGQPVTIPPI
                     RG"
     gene            complement(3506..5641)
                     /gene="ligA"
                     /locus_tag="PKV49_02115"
     CDS             complement(3506..5641)
                     /gene="ligA"
                     /locus_tag="PKV49_02115"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPL22437.1"
                     /translation="MQNNLAIIKIRVEKLRQQIEEARYAYHVLDKNLINEGALDSLKN
                     ELFQLEQQYPQLITADSPTQRVAGQVSEKFAKVVHRQPMISLYDAFSEEDMLSWQERN
                     HNFLGQKLEVDYYAELKLDGLAISLRYERGQLIQGATRGDGQVGEDVTTNIRTINSIP
                     LRLRQPTDQELRAIGLSKAEIDKLRIILDNDFLEIRGEAVMTKKNLADINQRYQSLGK
                     PLLANVRNAVAGSIRQLDPRITAERRLDFYAYDLLIADWPRGQVIAKRHQADRLANLL
                     GFKTLPQNQVCANLAVVFEFYRQMEQQRSVLPFEIDGTVVKYDDLRLWNQLGVVGKAP
                     RYMMAYKFSALQATTIVEDVVWQVGRTGVLTPAAVLKPVKVGGVTIARSTLHNFNEIK
                     RLGLLIGDTVILERAGDVIPKIVEVLPKLRSGQEKEITPPRYCPFCNGLVKKVTDQVA
                     YYCPNKNCYAVNLRRLRHWASKGAADWDGFGPKIIEQLVEQGLVENIADFYSLTIDQL
                     KILPGLGEKSASNLVDAIKQKKTLAIDRFIYALGIRQIGQESATTIADFLASRLKLTS
                     GEHNLLPDRLAVAAGALTLEDWQNLPDIGPKVAQSLTEWFSDPINLKLINLLTAQGVR
                     LSYSQPLVGQQPLAGRSFVLTGSLNSLTRSQAEATIKQAGGHITSQVSRQTDYVVAGR
                     DPGSKLAKAKQLGIKIINEEELLTLLKRS"
     gene            complement(5645..6286)
                     /locus_tag="PKV49_02120"
     CDS             complement(5645..6286)
                     /locus_tag="PKV49_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HPL22438.1"
                     /translation="MKTINSAAKWRDRYSGVVMIVSLLIITGAFIISLSIASLVMVAI
                     GISRTQGDSVRAYFAADSGLERALALDKIESIDKRNCLGENEDLCCDEPGECLFFDKT
                     PSGREDLVPVGCRPCADINNEPQYISEDHNQSYQIIFRGVDNDNSRLWLESTGNSNGA
                     KRSIKLTINRTAGCVPVCSDGTIQYECGYNSCNGFCGQCQTGEHCSNFLCVPD"
     gene            complement(6261..6806)
                     /locus_tag="PKV49_02125"
     CDS             complement(6261..6806)
                     /locus_tag="PKV49_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPL22439.1"
                     /translation="MTKRPVTGFTLIESMVAVAIFVLLIVSATNIFLSIVRSQRNTLS
                     SKNAQESVSYALEVMTKELRMAKIDDGSCGAAESVYTVSADGDQIRFRNYQDICVTYS
                     LDNGRLKIRRADDEAYMTPANIVISKLNFQASNLDIITQQPLITIRFVLNYFNTEAGQ
                     QSLQVQTSVSSRFYENYQFGG"
     gene            complement(6799..7326)
                     /locus_tag="PKV49_02130"
     CDS             complement(6799..7326)
                     /locus_tag="PKV49_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPL22440.1"
                     /translation="MNKQNNKTTKPAFSLIELLVSMAMVGVGLVGVASLAAQNIRVQY
                     VNRNSLISAQLAQEGLELARNRRDNNWKSTSPATSLDDIYDPGFIIDYTPDSYRSGYS
                     DIAETDPLRINGDGFYSYSGETITPFRRFLTSECQTDETIQAEYCLIRCFVSWQGKGQ
                     TGTYQAQDFIYSWYD"
     gene            complement(7319..7888)
                     /locus_tag="PKV49_02135"
     CDS             complement(7319..7888)
                     /locus_tag="PKV49_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HPL22441.1"
                     /translation="MKIFQEKEISQPAFTLVEIIIVIGIFVGLVSATIANYRTGSHQS
                     QLRLAAQELATNIRLAQSYAIGSKDFVDPNGSDHTSQSPQGGWGVYASLDNPNYYQIV
                     VDLDNDHVVDKSFRQINLPNNVVIDQIEDNSGTVNAGIVFFKPPEPQTFINGQAGNQI
                     TFTLKDQVGATTSQVIVNFFGLVEVKQNE"
     gene            complement(7894..9195)
                     /locus_tag="PKV49_02140"
     CDS             complement(7894..9195)
                     /locus_tag="PKV49_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22442.1"
                     /translation="MRKIIISSLVLLLVGLMVTRIPSVNAGANDDLFGWAWSPVIGWI
                     SFNNCVYGGGCSGINYGVKLEPSGDYPLSGYAWNSNVGWILFEDSYPTSAQPQHGVQL
                     DLATRKFSGWAKILALGDDGWIKLAKNSYDSGPDYWSQLNDDGKLAGWAWNNTIGWLS
                     FNSNDVTSSITYYVTGKAPTIPSNVIVTPDDVDGCNTLIIDWQDSLYAKDYSVFRSTS
                     ASFTPSATVNFIASTTVSNFIDNNNFSGLSLNTDYYYHIGASNFFGTTYTTASAHGKT
                     RPICGIDTITGEGDCLNGNPSVIKLNWQVPTVAPGTEIDHYQIKRCQIDDDPAGDQCL
                     QNSPNFVEVANTSDCYQVNPTSPDYPPTPPSENHCVGINCHCWERFTGDEVARQYVYK
                     IVAVDTMGNYGDDTATLINIYPCARQKSWRWEEKNPGFWDN"
     gene            complement(9200..10012)
                     /locus_tag="PKV49_02145"
     CDS             complement(9200..10012)
                     /locus_tag="PKV49_02145"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPL22443.1"
                     /translation="MTLLIIGLISSFFLGAALASFVHCWAHRLYNGEGFKRRSYCPNC
                     QHPISWFNNLPIFSYWRLKGHCAYCHQPIAKDYLISELIGGLLLASATAIVWFNLGGE
                     ANFWLAARTSHFWLTLIQALIGFILLMLVFWSDYWWMSIFVNPLLIGAVIVAGLNLLA
                     GRTWQSLVGGILFGGLFFALQFWLSKGRWLGEGDIYLGLFLGSWLGWPLIIPAIFIAY
                     VVGAIIGLVLLVVGLKRRQSQLPLGVFLAPSGWLVFLFGQSWWQAYLHWLGW"
     gene            complement(10129..10560)
                     /locus_tag="PKV49_02150"
     CDS             complement(10129..10560)
                     /locus_tag="PKV49_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPL22444.1"
                     /translation="MKKGFTLIELLVVIAIIALLSTLSVVALNSARVKSRDARRLSDI
                     KQLRTALEMYFDQNQSYPTTTGALGSGNFACLNSAGWAAVNCSGTVYMQKVPTDPKNS
                     TPFVYTYTPQGSGATSYTIAYALEGPSTTATATPDTIGYNQ"
     gene            complement(10633..11055)
                     /locus_tag="PKV49_02155"
     CDS             complement(10633..11055)
                     /locus_tag="PKV49_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPL22445.1"
                     /translation="MKKAFTVIELLVVISIIVVLSTLSVVSLGNARQRSRDTQRISDI
                     TQIQTALDKYALNRTDGLYPVTSSANIQGLCLDNTGFVPVCGGIVFMEKIPSNPTPSG
                     TDYIYNSDGMSYTLTYWLEGKTGSFPAGAATATPGGMQ"
     gene            complement(11057..12256)
                     /locus_tag="PKV49_02160"
     CDS             complement(11057..12256)
                     /locus_tag="PKV49_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPL22446.1"
                     /translation="MIFRYEAIDSQGVNKVGLVEADTSQEAILLLNENGLSLLKLDVA
                     NVKTRITIPFLNRVSMKDVVVFARQFSIMISAGIAMVQALRILVNQTENPNLKKVIIE
                     IADEVDGGATLSDCLAKRPKIFSNFFVSVTKSGERSGKLDEVLNYLADEIGKDYDMIA
                     KIRGAMIYPAVVLVGMFAIGVVMMVFVLPKLMDVLTETGTALPLATRILIAVSNAITG
                     YWWLILILFGGLIAAIRMALASRSGRRVFDVLVLRLPVFGKLFQKILLVRFCRSMNTL
                     LLGGVTIENSLAIAGDVVSNLTYKDLIEKTIHSVEDGNSISTIFLESSLIPKMVSYMM
                     QVGEESGKLDVIFNKVAEFYNREIDNMLANMMSLLEPLVMILIGVGVGIMVAAVIMPM
                     YSMVGNF"
     gene            complement(12262..14013)
                     /locus_tag="PKV49_02165"
     CDS             complement(12262..14013)
                     /locus_tag="PKV49_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPL22447.1"
                     /translation="MNKQSSNKLLDFLISAALINDSQLASVNQALTVKGAKLPDILVE
                     QGILDIERLTEIQASLAGLSYANLANYDIDEATLNVLPAEVANNYQVVCFHRDNNEIQ
                     VGLLDPENFKAIEAVDFLAKKNNLQARYFLVSPISFNIAYKKYQTLNKEISSALELKA
                     KEEEKEIKKAAEDEGLRFDEITKSAPISKIISATIRHGVELGASDIHIEPLPKESRVR
                     YRVDGILTTSLVLPRNIHDALVSRVKVMAKLKLDETRIPQDGRISLNIDGRDIDFRVS
                     VMPLMGQEKVVMRILDVSKGAPTLESLGFANNHYVVLKKAIERTSGLILITGPTGSGK
                     TTTLYSLLTILNKDNVNISTLEDPVEYFLKGVNQSQVRPEVNFTFATGLRSILRQDPD
                     IMMVGEIRDNETAELAVHASLTGHLVLSTLHTNDAVGAVPRLLDMKTEPFLLSSSLVL
                     VVAQRLVRLNCTHCLTEEIVGPDMLTEIRQAMAKVPADILQERLPNYDPDNLKFTRGT
                     GCAYCGQTGYHSRSVIAEILEINDQVKEMILEHKSFNQEAVKATQPFITMEQDGFIKA
                     IQGITTVEEVLRVIQSD"
     gene            complement(14017..14433)
                     /locus_tag="PKV49_02170"
     CDS             complement(14017..14433)
                     /locus_tag="PKV49_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22448.1"
                     /translation="MADILKAKHKTQKKSSKMFWLILAIAINSAGLFLVVRWYGQFTD
                     NQRPLTDIDETAFKVFTDKTSDNKTISPGQRQLLKTNLSKQTVEEFLSLSLLTDPKFK
                     NLQLNYQPVEVATTTDGETADDLNGLVIGNPRPFRY"
     gene            complement(14437..15105)
                     /locus_tag="PKV49_02175"
     CDS             complement(14437..15105)
                     /locus_tag="PKV49_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22449.1"
                     /translation="MAHLLNNNNNIDKKPANINAGSAGSIQVNNKLREFFVRKYQLFI
                     ALICLLIMAGSLLWFFLPQYRKTLAEANNNYQQAQQDLLDKQKYQTSLIQLNDFYKRI
                     DQKTRNKVIDILPAKIDRENLLAEIEAICLANSLLIKSSDVVVNEDNASTASSTINVA
                     SINNQLRSAIIKLELQGKDYHSFKSLLKTVENNLHLMDIQRLDYSPGKNQIVLEIQVY
                     YLSN"
     gene            complement(15108..16220)
                     /locus_tag="PKV49_02180"
     CDS             complement(15108..16220)
                     /locus_tag="PKV49_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22450.1"
                     /translation="MFFNRKATKTNNQASDSWEDQEAVKWSDTEGYEAVRQVNSGLRW
                     PSVWADRHAKVTNNQETKKKVEPAISSIRKVNKARSRQIKQKSDGLPLGAAQVNLSSF
                     QAKEERRRQMFEARRTNQSAPKVPEKPVSQPVPAAPAKHQASVSAVSHESNQQQRLQD
                     LKQKFEQKAWNRPSLTETSLVDSRDTLYFNWNKFWLILTQWLVVTLFFLSLCGALLYL
                     WQQRQNKQATDVTQRFNKIDQLINLTENEVSEVLDFRLRLIMVNEMLSKHIYWNNFFS
                     FLEKNTLPNVFYQGFSGDTKGDYILRAKTDNFDSMAKQIKAFRLSPDVLDVSSEGGEM
                     IKGETLTNASSTGETMPSNQLNFSIRLKIKPELFFR"
     gene            complement(16223..17302)
                     /gene="pilM"
                     /locus_tag="PKV49_02185"
     CDS             complement(16223..17302)
                     /gene="pilM"
                     /locus_tag="PKV49_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPL22451.1"
                     /translation="MGLLSSDLANGCLGVDIGSSGIKIVELKRDNKRVVLSSYGFSES
                     LGGGNPLNAPVEATAKTINQIRQEAGITSRRVVAGLPNFSVFSSIITLVDVSEAGLAA
                     AVDAEARKVMPLPLEEMVLDWKKIALPVGQKMESNDTAADIRSGAKTITKILLTGAPK
                     ALVNKYVEIFKKAELNLIFLEGETIALIRSLIGNDKGTIMLVEVGSSTSDLVIIDQGV
                     PVFNRSLDLGGFTVTQSISKSLNIDLQQAEQFKYDLGVAGIRSAELPDALKTLVQSIA
                     SEIKYSLNLFQNNNGKTVEKIILTGGSAMMYNFIEYLSQEFDINIIIGSPWSLISYPL
                     NIKPLLDEIGPRLSVAVGLAMRGIN"
     gene            17544..18713
                     /locus_tag="PKV49_02190"
     CDS             17544..18713
                     /locus_tag="PKV49_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22452.1"
                     /translation="MTSPDITGGSITTIDNTGRLPQAGPGSNINPIGNATGSLPIGSD
                     VASDDPSFVANPNPNITPTAVGRITETSQLVSGPTVGQMINNTTGQIQYYNPADGKFY
                     GVDKNGKTSPLSNKVFHNVNTVTWSNDGNKAVLEYPDNSKIIYDFANKKQVTLPKHWE
                     DFDFSANSEQLVMKSLGMDRDSRYLAITNSDGSGGKIIEFIGDNDKRVYPSWSPNNQI
                     VGMYTEGIDYDRQTVYFLGLNNENFKSTVIPGRGFQQQWSPDGKRLLYSVYYSENQMK
                     PMLWIVDASGSQIGNNRQMLNVQTWAEKCTFANNNELYCAVPTSLDYGAGFLPSLSNQ
                     TPDQIYRINTQSGTKQLIAIPQNEVTIASLFVSPDGNNLYFTDKNNGSINKIRLR"
     gene            18718..19497
                     /locus_tag="PKV49_02195"
     CDS             18718..19497
                     /locus_tag="PKV49_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPL22453.1"
                     /translation="MAKKITKTSQQLTKTIDNPWIISLVLASAGLALFILINTATSWP
                     NILLANLNNQPTAEYTVQSNFQELAANEGNLTTKTAISPASLRGPIISPNDPSRGSDK
                     AIINIVYFSDFACSFCRQQETIIKQALEQYPDQVRVIWKDLPEINPSSQSYQAAKAGR
                     CAFQQNKFWLFHDQLLSLKSLQNRDLEQAASMSGLDMAAFRQCLNRDQTAEAMVDNNL
                     KEADALGVSGTPYIYVNQRDFLGQISWEDLQNAIEAELAIQ"
     gene            19525..20892
                     /gene="radA"
                     /locus_tag="PKV49_02200"
     CDS             19525..20892
                     /gene="radA"
                     /locus_tag="PKV49_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPL22454.1"
                     /translation="MAKTIFACSKCGAQSPKWAGRCSECGAWGTLSEELPANPDSRAQ
                     QKKQALAGVKPSELLDWSSLPSNPAKRYTTNINEIDRVLGGGLVPGSLILLSGEPGCG
                     KSTLVAQIAQALEKELSIIYVSGEESGQQIKLRLDRLGYQPQKMAFASETNAEIIMAT
                     TLKNQPQLLIIDSIQTVYSDQVPSEPGSVNQIKACTIKLLELAKEQNIAIILIGHLTK
                     DGAVAGPKTLEHLVDTVIYLEHDSRHNYSILRASKNRFGSINEIGLLEMTNRGFRQLE
                     KTTSLFIQPTQGAAGSAISCLMEGSRPFLLEVQALVSKTFFGYPQRKAVGFDSNRLQI
                     LTTVISKQSKINLSNQDVIVSVAGGLKINETALDLAVCAAIASSYEDKPLPAKSLFLG
                     EVGLAGEIRPVGQLEPRLKEALKIGLVQAFLPSQAKITSSKMTINTTNNLTTFFNQLW
                     NKPSK"
BASE COUNT         6425 a         4849 c         3803 g         5884 t
ORIGIN      
        1 ttttcagcta taccgctata atttttaacc ttgcatggtt caataaactc atccgtttcg
       61 ccttgttgat gtttagcagt aattattgct agcggatcag taaattcact agcctgacct
      121 tcaactattg cctgataagc tttctcagtt ctatcccagc gctgatcacg atccatggca
      181 tagtaacggc cagaaattgt agcaatctga ccaaaaccca attcttttaa tttatcttgc
      241 actgtcgcaa catgaatcag gctttcagta attggcgcat cacgaccatc agtaatcaca
      301 tgaatcaaaa cattatgaaa attttctttg tggcaaaggt caagcaaggc cagcaagtga
      361 ttaaggtggg catgaacgcc ttgagtttgg agtaagccga ttagatgaag ttttttattt
      421 tgcgtttggc aatttttaat aacgcttaaa aaggcttgat ctttaaaaaa actaccatct
      481 tcaatggctt tattaattct aaccagtgat tggaaaatta ttcgtcccgc ccccaaggtc
      541 atgtgaccaa cctctgaatt accctgataa ccatcttcta agccaaccgc ctcaccgcta
      601 gcttgaagta ggctatgcgg ataagtgcta agatatttat tttcattagg cgtttgagct
      661 tgtctaatat aatttccctc cacttctgga ttataacccc agccatcacg aataattaaa
      721 actacttttt tcttgtcagt catattctta tgatttattt atctaactac attgtaactg
      781 attttattaa aaacaaaaac agtcaccttt ttggggtaac tgtttcgagc agcggaagga
      841 cggggattcg aaccccggag gcctgtgaca gcctactcgc tttccaagcg agcgcactcg
      901 accactatgc gatccttcct taaagcgcta acgcgcttat tattttattg ttgacggctg
      961 gcttgttgaa ctagttttaa taatcccggc tcggtctgac catcgctgcc gacaaaatca
     1021 gccaacggat aaacaccact taataaagtc ttgtttaaga aactgctagg cacgccaatc
     1081 gcccctaaag cctcggcttc agctgattgt ttgtcaattg tagcttggac ttctggttta
     1141 gtcaaacatt tttcaaattc agttaagccc aatccgacat tctgagcaat cgccgtccaa
     1201 tcgtcaaaag gtttttcttg actggccgtg gccgccacct gctcggcgaa ttcccaaaat
     1261 ttattctgtt gaccagcgca ttcagcggca gccgccgctt cttgagctaa ttgattattg
     1321 gccaacggat gctgacgcca agcaatcacc accgcatcgc caacttgttc tttaagttga
     1381 cgaacgattg gcaagaagct agccgctaaa ggatcagtca ggtcgctata gacaattaat
     1441 tgcgctggcg cttccgcttg tcccaataaa tgatcgtcgc ttttgattgg cggaacttcc
     1501 tgcaattcca ctgtcacatt ctgccaagcc tcttggttag acgcttgaat gtctttaatg
     1561 atttcagcct gttcatcatt attaacggtt actgatggct tgctcaggct ctgatagatt
     1621 tttaaagcga caaaaacgca aaagataact accaaaacaa tcaacaaaaa actatcagta
     1681 aatttacgtt gtttagtttt tacttggcca aacatattgt tatacttatt tttcccaagc
     1741 tagccgccgc cttaaacaag gcaaagctgt ttgcatagct tgagttaaag ttaataaatc
     1801 atagtcattc cccagcggac cgatcaactg gctgccaatc ggtaagccat cgccactcca
     1861 accagtcggc cacgatactg ctggcacgcc agctaatgaa gctggcgtta atagagcatc
     1921 ttccaaatat aggctgagcg gatcattaat tttttgacta agtcggaacg ctggtcgcgg
     1981 cacagtcgga gttagcaaga agtcaacttt ttcccaggcc tgatcaaaat cataactaat
     2041 ccagcgacga acccgctgag cttgattata ataaaaacaa ccatcgccag cgcttaaaat
     2101 atatgtcccg agcataatcc gccgcttagc ttccgctccg aagtgaccgc ggttaattag
     2161 ataagtttca ttaagactgg ccgctgattg cttataacca taacgcaacc catcaaaacg
     2221 ggctaaatta gcactgacct cggctgaagt aataacatga taaacggcta cgccatattt
     2281 ggttgatggc aaacttaacg ggataatttt cgccccatgt tcttgtaaaa aaattttgac
     2341 ttgcgtcagg ctatccaaaa ccgccaagtc taatccggtt gacacatatt cgtccggcca
     2401 accaattctc aggtcagccc aattaatctt gtctttgatt ttagccgtcg ctaatttggt
     2461 ttgcgcggtc gcgtcaaact tatcttgacc ggcaattatt cgtaaaacgc tggcggcatc
     2521 actggccgtt ttagctaaaa taccaataac atcagtcgaa ctagccatag ctatcaatcc
     2581 gtggcgagag attgctccat aactgggctt ataaccaaca acaccgcata aactagccgg
     2641 atgacgaacc gatccgccgg tgtcgctacc taaggcaaaa agacagaaat cagccgccac
     2701 ggccgccgct gaaccgcctg atgaaccgcc cggcactcgt tctaaatcat aaggattttt
     2761 ggttggcccc atcgcgctat tctcggtaga agtgccatga gcaaattcat ctaaattggc
     2821 tttgcctaat aaaaccgccc cggattgtcg taatttagca atcaccgtcg catcataagg
     2881 agcaatataa tcagccaata tcttggaggc agcagtcgtt ttaagaccct tggttaaaat
     2941 attgtcttta actaaatacg gaatacccaa tacccctgtc tggtctcctg agattagtct
     3001 ctggtcagct tcatcagcct gttttaatgc ccccgtttca tcaattaata agctagcgtg
     3061 gacttggtcg tcatattttt ttagacgccg caaataatat tcggtcaatt gccggctagt
     3121 aatctgacga ttatctaata attgtctggc agcagccagt gttaaattat ttaaatccat
     3181 aattatcccc tgattggcgg tatcgttact ggttggcccg gctgactacc ggtcatagcc
     3241 aaactggcgg ctgtttcgtc ggctggccaa gtagcgactt catcggccga caagtctact
     3301 gacgcagctt tgaactgata gccgtcaatt tgatcagtta attgcgcctg ctgaataaac
     3361 ttgtcggctt cacttaaatt attcaaatga ccaataatat cacttaattg ttggctgtaa
     3421 atttcggctt cgccgtcttc cagcttaatc ttggctagat tggccaaatg actgattgtt
     3481 tgtttatcca atttcatatt catcactaag accgcttaag caaagttaat aattcttctt
     3541 cattaataat cttaataccg agctgcttgg ctttagctag cttgctgccc ggatcgcggc
     3601 cggccaccac ataatcagtc tgccggctga cttggctagt aatatggccg ccagcttgtt
     3661 ttattgtagc ctcggcctga ctgcgtgtca aactattcag actgccagtt aaaacaaaac
     3721 tgcggccagc taatggctgt tgaccaacca atggttggct atatgataat cggacccctt
     3781 gagccgtcaa aagattaatc agttttagat tgattggatc cgagaaccat tcagttaatg
     3841 actgggccac ttttggaccg atatctggta aattctgcca atcttctaag gttagagcgc
     3901 cggcggcgac ggctaagcga tccggtaaaa gattatgttc gcctgaggtt aattttagcc
     3961 ggctggccaa aaaatcagcg atagtcgtcg ccgactcttg gccaatttgc cgaataccca
     4021 aagcataaat aaaacgatcg atggctaaag tctttttttg tttaatggcg tcaactaaat
     4081 tgctggctga cttctcacct agtcccggca aaatcttgag ctggtcaatg gttaagctgt
     4141 aaaaatcagc aatattttct accaacccct gttcaactaa ctgttcaata atcttcggtc
     4201 cgaaaccatc ccagtcagcc gcccccttgc tggcccaatg acgcaagcgc cgcaaattga
     4261 cggcataaca attcttatta ggacaataat aagcaacttg atcagttact tttttaacta
     4321 atccattgca aaatggacaa taccttggtg gggtaatttc tttttcctgg ccgctgcgca
     4381 attttggtag aacttcaact atcttgggaa taacatcacc ggctcgctcc agtatcaccg
     4441 tatcgccaat tagcaaaccc agccttttta tttcattaaa attatgcaaa gttgaccgcg
     4501 caatcgttac gccgccgacc ttaactggct taagcactgc cgccggagtt aaaacgccgg
     4561 tgcggccgac ttgccaaaca acatcttcaa ctatcgtcgt tgcttgaagc gcgctaaatt
     4621 tataggccat catataacgc ggcgccttgc cgacaacacc caactggttc catagccgta
     4681 aatcatcata tttaacaaca gtcccatcaa tttcaaacgg caagactgat cgctgctgct
     4741 ccatctgacg atagaactca aagaccaccg ccaaattagc gcaaacctga ttctgcggta
     4801 gggttttaaa acctaaaaga ttagccaaac gatcggcttg atggcgttta gcaattacct
     4861 gaccacgtgg ccaatcagcg attaacaaat cataagcgta aaaatccagc cgccgctcgg
     4921 ccgtaatgcg cggatctagt tggcggattg atccagccac cgcattgcgc acattggcca
     4981 gcagtggttt gcctaaagat tgataacgct gattgatatc agctaaattc tttttggtca
     5041 tcaccgcttc acctcggatt tccaaaaaat cattatctaa aattatccgt aatttatcta
     5101 tctcagcctt acttaaaccg atagcgcgca actcctgatc ggtcggctgg cgcaatcgca
     5161 atggaatact gttgatggtc cgaatgttag tagtcacatc ctcaccaacc tggccatcgc
     5221 cacgcgtcgc cccttgaatc aactgaccgc gctcatagcg caaactgata gctaaaccgt
     5281 ctaacttcaa ttcggcataa taatcaactt ccaatttctg gccaagaaaa ttatgattac
     5341 gctcttgcca gctcagcata tcttcttcag aaaaagcatc ataaagagaa atcatcggct
     5401 gccgatggac aactttggca aatttctccg agacttgacc ggctacccgc tgcgttggcg
     5461 aatcagcggt aattaattgt ggatattgtt gctcaagctg aaatagctca ttcttcaaac
     5521 tatctaaagc accttcatta attagatttt tatctaatac atgataagca tagcgcgctt
     5581 cttcaatctg ctgacgcaat ttttctaccc taatcttaat tatcgctaaa ttattctgca
     5641 taagttagtc gggcacacat aaaaaattac tacagtgttc tcctgtttga cattgaccac
     5701 aaaaaccatt acaactattg tagccgcact catattgaat tgtgccatca ctgcaaaccg
     5761 gcacgcagcc ggctgtccga ttgatagtca gcttaatcga tcgcttggcg ccattggaat
     5821 taccggttga ctccaaccac aaccggctat tatcattatc aacaccgcga aaaattattt
     5881 gatagctttg attatgatcc tcgctgatat attgcggctc attattaata tctgcacaag
     5941 gacgacagcc gaccggaacc aaatcctctc ttcctgatgg agttttatca aaaaacaaac
     6001 actcaccagg ctcatcgcaa cataagtcct cattctcacc caaacaattc cgcttatcaa
     6061 ttgattcaat tttatccaga gccaacgccc gctctaaccc gctatcggcg gcaaaataag
     6121 cccgcactga atcaccctgg gtccggctaa tgccaatcgc caccatcact aagctggcaa
     6181 ttgataaact aataataaaa gcgccagtga ttatcagcaa gctaacaatc attacaacgc
     6241 cactatatct gtctcgccac ttagccgccg aattgatagt tttcataaaa tcgcgatgat
     6301 accgatgttt gaacctgtaa agattgctga ccggcttcgg tattaaaata attcaaaaca
     6361 aaacgaatag ttatcaatgg ttgttgagtg attatgtcta aattgctcgc ctgaaaattc
     6421 aacttactga taacaatatt ggctggagtc atgtaggctt catcatcagc ccgtcgaatc
     6481 tttagccgac cgttgtctaa actataggtc acacaaatat cctgataatt acgaaatctg
     6541 atctgatcac catcagccga aacagtataa acactttcag cggcaccaca agacccatca
     6601 tcaattttag ccattcgtaa ctccttggtc attacctcaa gcgcatagct aactgactct
     6661 tgggcgtttt tagatgataa agtattgcgt tgtgaacgga caattgataa aaatatatta
     6721 gtagcactaa caattagcag gacaaaaata gctaccgcta ccattgattc aattaaagta
     6781 aatccagtaa ctggccgttt agtcatacca cgaataaata aaatcttgag cttgataagt
     6841 tccggtttgg cctttacctt gccacgaaac aaaacatcta attaaacaat attcagcctg
     6901 aattgtctca tcagtctgac attcactagt taaaaaacga cgaaaaggcg ttatagtctc
     6961 accgctataa ctgtaaaaac catcgccatt aattcttaat ggatcagtct cggcaatatc
     7021 gctataaccg gaacgatagc tatcaggtgt ataatcaata ataaacccgg ggtcgtagat
     7081 atcgtctaac gaagtagctg gactagttga tttccaatta ttatcacgac gattacgggc
     7141 taattccaaa ccctcctgag ctaattgagc cgatattagg ctattacgat taacatactg
     7201 aacccgaata ttttgagcgg ctaatgaagc aacgccaacc aagccaacac caaccatggc
     7261 catggacact aaaagctcaa tcagggaaaa agctggttta gttgttttgt tgttttgttt
     7321 attcattttg tttaacctca actaaaccaa aaaaattaac tattacttga cttgtcgttg
     7381 ctccaacctg gtcttttaaa gtaaaagtaa tctggtttcc ggcttgacca ttaataaatg
     7441 tctgcggctc cggcggtttg aaaaaaacta ttccagcgtt gactgtaccg ctattgtctt
     7501 caatctggtc tatcaccaca ttattaggca aatttatttg tctaaacgac ttatcaacaa
     7561 catggtcatt atctaagtca acaactattt ggtaataatt gggattatct agactggcat
     7621 aaacccccca tccaccttgt ggcgattggc tagtatgatc tgatccgtta gggtcaacaa
     7681 aatctttaga accgatggca taactctgag ctaaacgaat attggtcgct agctcttgag
     7741 cggccaaccg taattgtgat tgatgactgc ccgtccgata attagcaatc gtggccgaca
     7801 ctaggccaac aaagatgcca atgacgatga taatttccac caaagtaaag gctggctgac
     7861 taatttcttt ttcttggaat attttcataa aaatcaatta tcccaaaagc cgggattttt
     7921 ttcttcccaa cgccaactct tttgccgagc gcaaggataa atattaatta aagtggcagt
     7981 gtcgtcacca taattaccca tagtatcaac agcgacaatc ttataaacat actgcctggc
     8041 cacctcgtcg ccagtaaatc tttcccagca atgacaatta attccgacgc aatgattttc
     8101 tgatggcgga gttggcgggt agtcaggact agttgggtta acctgatagc aatcactggt
     8161 attagccact tcgacaaaat ttgggctatt ttgcaaacac tgatcaccgg ccggatcatc
     8221 atcaatctga caacgcttaa tttggtaatg atcaatctca gtgcctggtg ctacagttgg
     8281 aacttgccaa tttaatttaa tgactgatgg gttaccgttc aaacaatcac cttctccagt
     8341 aatggtatca ataccacaaa tcggtctagt cttaccatgg gcgctagctg ttgtataggt
     8401 tgtgccaaaa aaattactgg cgccgatatg gtaataataa tcagtattta aagataatcc
     8461 actaaaatta ttattatcga taaaattact aaccgttgtt gatgcaataa aattgacagt
     8521 tgctgatggc gtaaacgagg cgctagttga acgaaaaacc gaataatctt tagcataaag
     8581 actgtcttgc caatcaataa ttaaagtatt acaaccatca acatcatcag gcgtaacaat
     8641 cacgttactg ggaatagtcg gtgctttgcc agtaacatag taagtaattg aactggtaac
     8701 atcattagaa ttaaacgata accagccaat tgtattattc caagcccaac cagctaattt
     8761 gccatcatca ttcaattgtg accaataatc tggcccgcta tcataactat ttttagctaa
     8821 cttaatccaa ccgtcatcac caagagctaa aatcttggcc caaccgctaa attttcgggt
     8881 cgccagatca agctggactc catgctgagg ttgagcgcta gtcggataac tgtcttcaaa
     8941 caatatccag cccacattac tattccaggc atagccagat aacggataat caccactagg
     9001 ctctagctta acgccataat taatgccgct acaaccgccg ccataaacac aattattaaa
     9061 actaatccaa ccgattaccg ggctccaagc ccaaccaaac agatcatcat tagcgccggc
     9121 attaactgat gggatcctcg taaccattag gccaacgagt aataaaacta aactactaat
     9181 aattattttt cgcatattat taccaaccca gccaatgtaa ataagcctgc caccatgatt
     9241 ggccgaataa aaaaactaac cagccgctcg gcgccaaaaa tacccccaac ggtaattgcg
     9301 attgccgtcg cttaagtccg acaactagta aaacaaggcc aatgatggcg ccgactacat
     9361 aggcaataaa aatagctggt attatcagtg gccaacctaa ccaactaccc agaaataatc
     9421 ccaaataaat atcaccctcg cctaaccaac ggcctttaga caaccaaaac tgcaaagcaa
     9481 aaaataaacc gccaaacaaa attccgccaa ccagcgactg ccaggttcgg ccggctaata
     9541 aattaagccc agccactatg acggcgccaa ttaacaatgg attaacaaaa atcgacatcc
     9601 accaataatc gctccaaaaa actaacatca atagaataaa accaattaaa gcttgaatca
     9661 gagtcagcca aaaatggcta gtcctagctg ctagccaaaa attagcttca ccacctaagt
     9721 taaaccagac aatcgctgtg gcgctagcta ataataaacc gccaattaat tcactaatca
     9781 aataatcttt ggcaattggc tgatggcaat aagcacagtg tcctttgagc cgccaataac
     9841 taaaaattgg caaattattg aaccaactta tcggatgctg acagtttgga caatatgaac
     9901 gacgcttaaa accctcgcca ttataaagac gatgtgccca acaatgaaca aagctagcta
     9961 aagctgcccc taagaaaaaa ctactaatta aaccgataat cagcaaggtc atagatattt
    10021 ttacctattt attatagcaa taatcagccg tcaagacaaa tagccgacta cacaattaaa
    10081 ataaaaaaac accgctattt agcggtgttt ttaaattagc aactagactt attgattata
    10141 gccgatagta tcgggagtgg ctgtggctgt ggtggacgga ccctctaaag cataggcgat
    10201 agtatagcta gtagcaccgc tgccctgagg cgtataagta taaacaaatg gggtgctgtt
    10261 tttaggatca gttggcactt tctgcatata aactgtaccc gaacaattaa ctgctgccca
    10321 gccggcacta tttagacagg caaaattacc gcttcccaat gccccagtag tagtcggata
    10381 actttgattt tgatcaaaat acatctccaa tgcagtacga agctgcttga tgtctgataa
    10441 acgacgagca tcacgcgatt ttacccgggc gctattcaaa gctaccaccg ataaagtgga
    10501 taatagggca ataatggcga taaccaccaa aagctctatt aaagtaaaac cttttttcat
    10561 aaaaataatt ttataaatta ataatgaaaa tattaaaaat acaacctaat agtattatac
    10621 accaaattaa tttcattgca taccccccgg cgtcgctgtg gccgctccag ccggaaaaga
    10681 tccggttttg ccttctaacc aatatgttaa agtataactc attccatcgc tattgtaaat
    10741 ataatcggtg ccgctcggcg tcggattact gggtattttt tccataaaaa ctattccacc
    10801 acaaaccggc acaaagccgg tattgtccaa acaaagccct tgaatattag ctgaagaagt
    10861 cactggataa agcccatcag ttctattcag agcatacttg tccaatgctg tttgaatttg
    10921 tgtaatatca gaaatccgct gcgtgtcgcg agatctctga cgagcattac ctaaagaaac
    10981 aacagacaga gttgataaaa caacaataat tgaaatgaca actaatagct ctattaccgt
    11041 aaaagccttt ttcatattaa aaattaccaa ccatgctata catcggcata atcaccgccg
    11101 ctaccataat accgacacca acaccaatta aaatcattac taacggctcc aataaactca
    11161 tcatattggc cagcatatta tcaatttccc gattataaaa ttcagcaacc ttattaaaaa
    11221 tgacatcaag tttaccggac tcttcaccaa cttgcatcat gtatgaaacc atcttgggaa
    11281 tcaatgagct ttccaaaaaa atcgtagaaa tagaattacc atcttcaaca ctatggatag
    11341 tcttttcaat taaatcttta taggttaaat tgctaactac atcgccagcg atagctaggc
    11401 tgttttcaat cgttactccg ccaagcaaca gcgtattcat tgaacgacaa aaacgaacta
    11461 ataaaatttt ctggaataat ttgccaaaaa ccggtaaacg caaaactaaa acatcaaaca
    11521 ctcgacgacc ggatcgtgaa gctagagcca ttctaatagc cgctatcaac ccaccgaaca
    11581 gaattaatat taaccaccaa tatcccgtta tagcgttcga aacggcgatc aaaattctgg
    11641 tggccaatgg taaagcagtt cctgtttcag ttaaaacatc catcaacttg ggtaaaacaa
    11701 aaaccatcat cacaacaccg atagcaaaca taccaaccaa gaccactgct ggataaatca
    11761 tggcgccgcg aattttagca atcatgtcat aatctttacc aatttcatcg gctagataat
    11821 ttaacacttc atctaattta ccagaccgtt cgcctgattt agtaacactg acaaaaaaat
    11881 tagaaaatat tttgggccgc ttcgctaaac aatcagacaa tgtcgcgccg ccatcaactt
    11941 catccgctat ttcaataatc actttcttta aattaggatt ctcggtctga ttaaccagta
    12001 tccgtaaggc ttgaaccatg gctatgccag cactaatcat aatagaaaat tgccgagcaa
    12061 aaacaaccac atccttcatt gacactctgt ttaaaaacgg tatcgtaatt cttgttttga
    12121 cattagcaac atctagtttt aatagactga ggccattctc gtttagcaag agaatggctt
    12181 cttgactagt gtcggcctct actagaccaa ccttattgac cccctgacta tcaatggctt
    12241 cgtaacgaaa aatcataata attaatctga ttgaataaca cgcaatacct cttcaacggt
    12301 agttatacct tgaatggctt taataaaacc gtcctgttcc atagtaatga acggctgagt
    12361 tgccttaacg gcttcttgat tgaaactctt atgttctaaa atcatctctt taacctgatc
    12421 attaatttcc aaaatttcgg caataactga acgagaatga tagccggttt gaccacaata
    12481 agcacaaccg gtgccacgag taaatttcaa gttgtctgga tcatagttag gcaatctttc
    12541 ttgaagaatg tcagccggca ctttagccat cgcttgtcta atctcagtta acatatcggg
    12601 accaacaatt tcttcagtta aacaatgagt gcaatttaac cgaactaagc gctgagcaac
    12661 caccaaaact aaactagatg aaagtagaaa cggttcagtt ttcatatcta aaagacgagg
    12721 aactgcgccg accgcatcat tggtatgcag ggtggacaaa acaaggtgac cagtcagcga
    12781 ggcatggaca gccaattcag cagtttcatt atcacgaatt tcaccaacca tcataatatc
    12841 gggatcctga cgcaaaatag atcgcaagcc agtggcaaag gtaaaattaa cttccggccg
    12901 gacttgcgat tggttaaccc ctttcaaaaa atactcaacc ggatcttcta gagtagaaat
    12961 attaacatta tctttattga ggatagttaa taatgagtat agggtggttg ttttaccaga
    13021 tccggtcgga ccggtaatca aaattaatcc gctagttcgt tcaatcgctt ttttcaaaac
    13081 cacatagtgg ttattggcaa aacccagaga ttccaaagtc ggcgctcctt tactaacatc
    13141 caaaattctc attaccacct tttcttgacc cattaatggc ataacggaaa cacggaagtc
    13201 gatatcgcgc ccatcaatat ttaaactaat gcgaccatct tgcggaatgc gggtttcatc
    13261 cagctttaat ttagccataa ctttaacccg cgaaaccaga gcatcatgaa tattgcgcgg
    13321 taaaaccagt gatgttgtta aaataccgtc aacccggtaa cgaacacgac tttctttagg
    13381 cagcggttca atatgaatat cgctggctcc cagctcaacc ccatggcgaa tggtcgccga
    13441 aataattttg gatatcggag cgcttttagt aatctcatca aaacgcaaac cttcgtcctc
    13501 agcagccttc ttgatctctt tttcttcttc cttggctttt aattctagag cgctggaaat
    13561 ttctttattt aatgtctgat attttttata ggcaatatta aaactaatcg gcgaaactaa
    13621 aaaataacga gcttgcagat tatttttttt cgccaaaaag tcaactgctt caatcgcttt
    13681 aaaattctcc ggatctaata agccgacttg aatttcgtta ttatcacgat gaaagcaaac
    13741 cacttgataa ttgttagcta cttcggctgg taaaacatta agagtggctt catcaatatc
    13801 atagttagcc aaattagcat aagataaccc agccaaactg gcttgaattt ccgtcaaacg
    13861 ttcaatatct aaaatacctt gttcaactaa aatgtctggt aatttagccc ccttaactgt
    13921 caaagcctga ttaacgctag ccaattgact atcattaatc agcgcggccg aaatcaaaaa
    13981 atctaataat ttattgctgg attgcttatt cataatctaa taacgaaatg gccgcggatt
    14041 gccaatcact agcccattta aatcatcagc ggtttcacca tcggtggttg tggctacctc
    14101 tactggctga taattaagtt gcaaattctt aaatttagga tctgtgagta agcttaaaga
    14161 taaaaactct tccactgttt gtttactgag attggtcttt aataattggc gctggccagg
    14221 gctaatggtt ttattgtcgc tggtcttatc ggtaaaaacc ttaaaagccg tttcgtcaat
    14281 gtcagtcagc ggacgctgat tatcggtaaa ttgcccatac caacggacaa ccaaaaatag
    14341 accagctgaa ttaatagcaa tagctaaaat tagccaaaac attttactgc tttttttctg
    14401 agtcttatgt tttgctttca aaatatcggc catagattaa ttcgacaaat aataaacctg
    14461 aatttccaac actatttgat ttttaccggg agaataatcc agacgctgaa tgtccattaa
    14521 atgtaaatta ttttccactg tttttaatag acttttaaaa ctatgataat ctttaccctg
    14581 taattctaac ttaatgatag ctgatctcag ttgattattt atactggcca cattaattgt
    14641 actgctggcg gttgatgcat tatcttcatt aacaactacg tcagatgatt taatcaacag
    14701 gctattagcc aaacaaatag cttcaatctc ggccaacaaa ttttctctgt cgatcttggc
    14761 tggtaaaata tctataactt tattgcgagt tttttggtca attcgcttat aaaaatcatt
    14821 caactgaatc aaactagttt gatatttttg tttgtccaat aaatcctgtt gagcttgctg
    14881 atagttatta ttagcctcag ccagtgtttt tcgatattgt ggcaaaaaga accacaataa
    14941 gctaccagcc attatcaata aacaaatcaa agcgataaat aactgatact tcctaacaaa
    15001 aaattcacgc agcttgttat taacttgaat tgacccagca ctgccggcat ttatattggc
    15061 tggttttttg tcgatattat tgttgttgtt taataaatgg gccatattta tctaaaaaac
    15121 aattcgggct tgatcttgag acgtatagaa aaatttaact ggttgctggg catagtctcg
    15181 ccagtactgc tagcgttagt taatgtttca cccttaatca tttcaccgcc ttcactacta
    15241 acatcaagaa catctggcga taaacgaaaa gcttttatct gcttggccat gctgtcaaaa
    15301 ttatcagttt tagctcgtaa aatatagtcg cctttagtat cgccgctaaa gccctgataa
    15361 aacacattgg gcaaggtatt tttttctaaa aaactaaaaa aattattcca ataaatatgc
    15421 ttacttaaca tttcattaac cataattaat ctgagacgaa aatcgagaac ctcgctaacc
    15481 tcattttcgg tcaaattaat caattgatca attttattaa atcgctgagt aacatcggtc
    15541 gcttgcttgt tttgccgctg ttgccataaa tacagcaaag ctccgcatag tgacaagaaa
    15601 aatagggtaa caaccagcca ttgagttagt atcaaccaaa atttattcca attaaaatat
    15661 aatgtgtctc gactatcaac taagctagtt tcagttagac ttggccgatt ccaggctttc
    15721 tgttcaaatt tttgcttcaa gtcttgaagt ctttgctgct gattgctctc atgactgacg
    15781 gctgatactg aagcttgatg cttagctgga gcggctggta ccggctgact tactggtttt
    15841 tctggcacct tcggcgctga ctgattagtt cgccgagctt caaacatttg gcgacggcgt
    15901 tcttctttgg cttgaaagct ggataaatta acctgggctg ctcctaatgg caggccatcg
    15961 gatttttgtt ttatttgacg gctacgcgct ttattgactt tgcgaattga actaatggct
    16021 ggttcaactt tcttcttggt ttcttggtta ttagtaactt tagcatgacg atcggcccaa
    16081 actgacggcc atcttagccc gctattaact tgacgaaccg cttcatagcc ttcagtgtct
    16141 gaccatttaa cagcttcttg atcttcccag ctatctgacg cttgattatt ggtttttgtt
    16201 gctttgcgat taaaaaacat aactagttaa tcccgcgcat cgctagacca acggccactg
    16261 ataagcgcgg accgatttca tccaatagcg gtttgatatt taaaggataa gaaattagtg
    16321 accatgggct gccaataata atattaatgt caaattcttg cgacaaatat tcaataaaat
    16381 tatacatcat ggccgagccg ccagtcaaaa taattttctc aacagttttg ccgttgttat
    16441 tctgaaataa attcaatgaa tatttaattt cactggctat cgattgcacc aatgttttaa
    16501 gggcatcagg caactcagct gatctgatac cagccacacc taaatcatat ttaaattgtt
    16561 cagcctgttg caaatcaata tttaaactct tgctaattga ttgagtaacc gtaaaaccgc
    16621 ccaaatcaag actgcgatta aacactggca ctccttggtc aataatcacc aaatcgctgg
    16681 tggatgagcc aacttccacc aacataattg ttcctttatc attgccaatc aatgatctaa
    16741 tcaaggcgat agtctcacct tctaaaaaga ttaggttcag ctcagctttc ttgaatatct
    16801 caacgtattt attaaccaaa gccttggggg cgccagttaa taatattttg gtaatggttt
    16861 tagcgccgct gcgaatatcg gccgccgtat cattgctttc cattttctga ccaactggca
    16921 aagcaatttt tttccaatct agaaccattt cctcgagcgg caagggcata actttacgcg
    16981 cttcagcatc aactgccgct gccaagccag cctcactaac atcaactaga gtaataatgg
    17041 atgaaaaaac tgaaaaattg ggtaatccgg ccaccacgcg acgactggtt atgccagcct
    17101 cttggcgaat ctgattaatt gttttagcgg ttgcctcaac cggcgcgttc aacggattac
    17161 cgccgcccaa agattcactg aatccataag atgataagac aacgcgtttg ttatcgcgtt
    17221 tcaattccac aattttaatt cccgagctac caatatctac accaagacaa ccattagcca
    17281 aatcagaact taataaaccc ataatcaaca aaaggttaaa aaaaataaac tttgacggta
    17341 atcaatcaat cttctataat aataacagat tattgatatt atagcacatt cgaccaatcg
    17401 accacaatag cttaatttaa taaaaaaact aactgttgcc tatgaatttt ttttcaaaat
    17461 ataaaaagct aatcttagtt atccttttta ttgcttttgt gttaggagca gcttatctgc
    17521 tatatatctt ttttttccgc gatatgacca gcccagatat aaccggcggt tcaatcacga
    17581 ccattgataa tactggccga ttaccccagg ctggtccggg gtccaatatt aatccaattg
    17641 gaaacgccac cggcagccta cctattggct cggatgttgc cagcgatgat ccgtcttttg
    17701 tggctaaccc taatccgaac atcaccccga ctgctgtcgg tcgcataacc gaaacaagcc
    17761 aactagtcag tggcccaact gttggtcaaa tgattaataa cacaaccgga caaattcaat
    17821 attataatcc cgccgatggc aaattttatg gcgttgataa aaatggcaag actagtccgc
    17881 tcagcaataa agtttttcat aatgttaata cagtgacctg gtccaatgat ggcaataaag
    17941 ctgttttaga atacccggat aacagcaaga ttatttatga ttttgccaat aaaaaacagg
    18001 ttactctgcc aaaacactgg gaagattttg atttctcagc caacagtgag caactggtta
    18061 tgaaaagcct gggcatggat agagatagcc gctatctggc cattactaat agcgatggta
    18121 gcggtggcaa gattattgaa tttatcggcg acaatgacaa acgcgtctat ccaagctggt
    18181 cgccaaacaa ccaaattgtt ggaatgtata ccgaaggcat tgactatgac cgccaaactg
    18241 tttatttttt aggacttaat aatgaaaatt ttaaatcaac agtaatcccc ggacgcggtt
    18301 ttcagcagca atggtcgcct gatggcaaac gcctgctcta tagtgtttat tacagtgaga
    18361 accaaatgaa accaatgcta tggattgttg atgcctctgg cagtcaaatt ggcaacaacc
    18421 gacagatgct caatgtccaa acctgggcgg aaaaatgcac tttcgccaac aataacgagc
    18481 tttattgcgc tgtgccgact agtcttgatt atggcgccgg ctttttaccc tcattatcca
    18541 atcaaactcc tgaccagatt tatcgaatca acactcaatc cggaaccaaa caactgatag
    18601 ccatcccgca aaatgaggtt acgattgcta gtttgttcgt cagtcccgat ggcaacaacc
    18661 tttactttac cgacaaaaat aatggatcca ttaataaaat aagattacgc taacaatatg
    18721 gccaaaaaaa ttactaaaac cagccagcaa ttgactaaaa ccatagacaa cccttggatt
    18781 atcagtttag ttttagcaag cgccggcttg gctttattta tattaataaa cacggcgacg
    18841 agctggccga atattctgtt agctaatctc aataatcagc caacggctga atataccgtg
    18901 cagtcaaatt tccaagagct ggccgccaat gagggtaact taaccactaa aaccgccatt
    18961 agcccggctt ccttgcgtgg gccaattatt tcacccaacg acccaagtcg cggctctgac
    19021 aaggccataa ttaatatcgt ttattttagc gattttgctt gcagcttctg tcggcaacaa
    19081 gaaactatta ttaaacaagc tttggagcaa taccctgacc aagttagggt aatttggaaa
    19141 gacctaccag aaatcaatcc aagcagtcaa tcatatcagg ccgccaaagc cggccgctgt
    19201 gcttttcaac agaataaatt ctggttattc cacgatcaat tattgtcatt gaaatctttg
    19261 caaaatcgtg atttggaaca ggcagcctcc atgtccggct tggatatggc cgccttccgc
    19321 caatgcttaa atcgagatca gacagctgaa gctatggttg acaacaatct aaaggaagct
    19381 gatgccttgg gtgttagcgg aacaccctat atttatgtga accaacgtga tttccttggt
    19441 cagatcagct gggaagactt gcaaaatgcc attgaagctg aattggctat tcaataataa
    19501 ttagttattc ctcccgcaat cattatggct aaaactattt ttgcctgcag caagtgcggg
    19561 gcgcagtcgc caaaatgggc cggccgctgc agtgagtgtg gcgcctgggg aactttaagt
    19621 gaagaattac ccgccaaccc cgacagccga gcacaacaaa aaaaacaagc cttagccggt
    19681 gttaaaccta gcgaattatt ggattggtct agtttaccat caaatccagc taaacgttat
    19741 acgaccaaca tcaacgaaat cgaccgcgtc cttggcggcg gcttagtccc cggctcgcta
    19801 atcttattaa gcggcgagcc tggctgcggc aaatcaaccc tagtggctca aatagctcaa
    19861 gctttggaaa aagaattgtc tattatttat gtcagcggcg aagaatctgg acaacaaatc
    19921 aaactacgcc tagatcgctt aggctatcag ccacaaaaaa tggcctttgc cagcgaaact
    19981 aatgctgaga taattatggc caccactctt aaaaatcagc cgcaattatt aatcattgat
    20041 tctatccaaa ccgtctattc cgaccaagtt ccttcagagc cgggtagcgt taatcaaatt
    20101 aaagcttgca ctattaaatt attagaactg gccaaagaac aaaatatcgc tatcatttta
    20161 attggccact taactaagga cggtgcagtg gccggaccaa aaaccttaga acacttagta
    20221 gatacagtta tttatttaga gcatgattcg cgccataact atagcatcct gcgcgctagt
    20281 aaaaaccgct tcggctccat caacgaaatt ggcctattag aaatgactaa ccgcggtttt
    20341 cgccaattag aaaaaactac tagtctgttc atccagccaa ctcaaggagc cgccggatcg
    20401 gctatcagct gcctaatgga gggatctcga ccgttcttac tagaagttca agcgctagtc
    20461 agtaaaacat tttttggtta tccgcagcgc aaagcagtcg gctttgacag taatcgttta
    20521 caaatcttga caacggtgat cagcaagcaa agcaaaatca atctatctaa tcaagatgtt
    20581 attgtcagtg tcgccggcgg tttaaaaatc aacgaaacgg cgcttgattt agccgtttgc
    20641 gccgctattg cttccagcta tgaagataag ccgttgccgg ccaaaagcct atttttaggc
    20701 gaagtcggtc tggccggtga aatcaggccg gtcggccagt tggagccccg tctcaaagaa
    20761 gctcttaaaa tcggcttggt gcaagctttt ttaccgagtc aagctaaaat taccagctct
    20821 aaaatgacta tcaatacgac taataacctg acgacatttt ttaatcagct ttggaataaa
    20881 cccagtaaat aaagccttta ctattattgc ggcagccagc cgctagccct gtccgactcg
    20941 gttaagcggg cagctattgt t
//
LOCUS       DAOPAI010000012        20895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_2668_length_20895_cov_4.807965, whole genome shotgun sequence.
ACCESSION   DAOPAI010000012 DAOPAI010000000
VERSION     DAOPAI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20895
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2668_length_20895_cov_4.807965"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKV49_02205"
     CDS             complement(<1..409)
                     /locus_tag="PKV49_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPL22455.1"
                     /translation="MLYLYLFLSLTVISSASLIGIISLRWSTDKMARINFWLISIAAG
                     SLIGDSFFHLLPEATEGRTGLTPWLYLTGGMLGFFVLEKIIHWRHCHIPTSSQHPHPL
                     GTMNLVGDFLHNFLDGLVLANAFLVSPILGLTTA"
     gene            561..1070
                     /locus_tag="PKV49_02210"
     CDS             561..1070
                     /locus_tag="PKV49_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22456.1"
                     /translation="MTTKIKKEDVLNNGNKSTVHHLIPRSRGGDSSKDNIKRVPWRIH
                     DAWHTLFNNLLPEEVCAQVITTPEDLFNTANKKMAWKVLFGCHWQEVQSGSAQEKILG
                     QIMTNWLPRNYIVSWDKINQLIWDNCCINCPRVSCCLTRYQKWPLEHKQVFYYCYLPV
                     KKALVALLI"
     gene            1176..3125
                     /locus_tag="PKV49_02215"
     CDS             1176..3125
                     /locus_tag="PKV49_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPL22457.1"
                     /translation="MKKRKKIWWIILGVVIVIISLVVWQGRKKNQPQYTTVAAEIGSL
                     KQTVSLTGTVKPVKELALNFLSAGRINNLPVKIGDQVSAGQLLAELDYGSLLLRKSEA
                     EAGLQIAQANLNKLLSGASQQAIAVNRSSVEQAISSQRMAQIELDKVKKTAAENIKQA
                     EKSVNDLYSSAADTPTVQEQAVITAQTNLDNAQRTGQQNINNSRSSGLIIFGDKIAAA
                     KIALDNINTILTDNNAKYVLSVKDSSYLQRTKEARNLALDNYAAVSQAVSAAKNASDT
                     QSFIVAANKAKQFLNQTALAINYSYSMLEATITSNNFTQGQLDNYKGIVSNQSSQING
                     AITAAETAAQNYHNAQSNSETTVASAQDSLEQARVALENAKLAAANNLSTVRLASDRQ
                     ISTAQSQLDNANKAVDVAKAQLASITAPARSADIALAQGQISQAQANLASAQKQIDDS
                     RLFSPLDGVVTQVNYEVGEQFSAAGKALIMVLVNNSFDIEVDVTEADINKIKVGNLVE
                     ITLDAFGDDTIFHGQVYFIEPAQTVIQDVVYYKVKISFSDSQEVKSLTEARQISIKAG
                     MTANTTITTDQRENIVKIPGRAIVEKDNGVKVVRILKDNKLQEIPAAIGLRGDEGLVE
                     VLGGVQPGDQVVTFIKYPKTAADKQ"
     gene            3133..3858
                     /locus_tag="PKV49_02220"
     CDS             3133..3858
                     /locus_tag="PKV49_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL22458.1"
                     /translation="MVNYPLIELQHLDKIYQSGDVVTNVLKDINFTINRGEFISIMGP
                     SGSGKSTLMHILGLLDRPTSGQYLFNHHNTTELSDDQLAALRNHDIGFVFQAFNLLTR
                     TSVLDNVKLPLLYSKDKERMDERATAVLESVGLGHRLHYFTNQISGGEKQRVAIARAL
                     VNDPEVIFADEPTGNLDSKSGNQVMQILQELNQQGKTIILVTHETFTAEHAQRIIRIR
                     DGQLVSDELVPQQRLASQGQVLK"
     gene            3860..5113
                     /locus_tag="PKV49_02225"
     CDS             3860..5113
                     /locus_tag="PKV49_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL22459.1"
                     /translation="MVIVEDIRQSLILAWRALMSNKVRSFLTMLGIVIGVSAVIIIMS
                     VGAGAQSLILNQLEGLGSDLIAVLPGQSDENGPPSQAFGITVTTLKYSDAQALQQAKN
                     IANVQAVNAYYQVPINASWRNNDYDVSLNGVTTSYLDVENGTVEQGRFFTDEEFNSAA
                     RVAVLGSAVADELFGSTDPLGQKIKIKNQTLEVIGVMKSRGKVGFQDYDDQVFVPLIF
                     VQKMIAGVDYVSFIRIKLKPGADINQALEEARQTLREQHNIDNSVDDDFSVRSFRDAV
                     AMITTITDALKYFLAAMAALSLLVGGIGIMNILLVSVTERTREIGLRKAIGATSNQIL
                     RQFLLESVCLTLIGGLLGIASGIAVSYLVAVVANLLEYKWSFIISLSALLLAVFISIA
                     IGVIFGLYPARRASQLDPIEALRYE"
     gene            5115..6344
                     /locus_tag="PKV49_02230"
     CDS             5115..6344
                     /locus_tag="PKV49_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL22460.1"
                     /translation="MRLQTALQLAIRSLRLNLLRTSLTVLGIIIGVAAIVVVFAAGEG
                     IKSLVVGEVAAYGTDIIETEAKTPTKKNTTPSAQVTTLKTSDMEAIDRLENVKYSYGA
                     IFGQQKVSWQSQNKRVFLFGVSATYTDIDKKSQPIEGRFFTAEEDRGQAMVAVLGYKL
                     RQELFNNQPAVGQWIKIGNGKFQVIGVLEERGGAVSMIDFDNTVYLPLKTLQKRILGV
                     DHVLYLMHQLNDVSRADDTAEEIKLLMRERHNITDPDKDDFLVTTMEELLKTLNTVTN
                     TITWLLLAIVVISLIVGGVGIMNIMYVTVSERTPEIGLRKALGATYRDIVSQFLVESI
                     LITIWGSLIGIIFGLIMAWILSLAANYVGLVWHFTIPWSGVITAIIFSLACGLLFGIR
                     PAQKAAQLDPVEALRAE"
     gene            complement(6345..7727)
                     /locus_tag="PKV49_02235"
     CDS             complement(6345..7727)
                     /locus_tag="PKV49_02235"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPL22461.1"
                     /translation="MTTKSFPQPISFIKMLGPSFIILALGLGSGEVILWPYLAANYGL
                     GIIWGALLGLTCQYFINMEIERYALVKGESVFSGLARLWHWVPYWFILSTFIGWALPG
                     IAAASAQIFGHLLGIQNFRWLAIGILLLIGAVLTFNQSVYKTLEKITKTILLVGIPCL
                     LILVIFLAKPTDWSALFQGLLGYGHNYRWLPSGVSLITFLAAFAYAGAGGNLNLSQSI
                     YIKDKGYGMGRYAQKLSGLFNINQTQTINLSGTDFVVNQTNLRRFRQWWKRISLEHLL
                     VFWLLGFLSMALLMILSYSTTYGLSGNLSGLNFILAEGRLIGQATAPLVGSAFLVLLG
                     LMLAQTQLGVLDSTSRIMSENAALLKLQNNPDKQLNLSKIYYRFLWGQIVFGISLFLL
                     NFYEPKQLIVAGAIVNALAMFVHIALVNIGNWKTLPPATQPGWPRRIIVLVIFLLFAA
                     FGFITLKSYL"
     gene            7779..8969
                     /locus_tag="PKV49_02240"
     CDS             7779..8969
                     /locus_tag="PKV49_02240"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPL22462.1"
                     /translation="MSEPLASAMRPNNLDNFVGQAEIIGPDSFLRQAIIKQHLPSLLF
                     WGPPGCGKTTLAKILANSLEADFIALSAVTSGLKELRQVIDQARGNQIANKPTILLVD
                     EIHRWNRGQQDALLPYVEDGTIILIGATTENPSFSVNAALLSRCQVVIFQQLTADDII
                     NLLERALKDKQCGLGKLNIKADPSALARIAQIADGDARLSLNLLEAAAKISSEINLEV
                     VNKISRQIVFSYNQAGDERYHLISALHKSLRGNDASAAVYWLVRMLESGEDPLYIARR
                     LVRFASEDIGLANNTALLLATSCFEACRQIGLPECKVVLTQAVIYLAKSPKSIAAYQA
                     YQRAAADVNQFGALPVPLHLLNASTKLAKEMGYGKNYKYTPLESDDGQSYWPDKLPAK
                     NYLA"
     gene            complement(8970..10136)
                     /locus_tag="PKV49_02245"
     CDS             complement(8970..10136)
                     /locus_tag="PKV49_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPL22463.1"
                     /translation="MQKTKSTFYLIYGLVFITALIDAMVGYVQSSFLNQFLPLEYIGL
                     FWSLVTIITIAISFIYPRLARRYSTYRLMIITYFLAVAANFIISSETTTWLILVSFIV
                     RYLSIVFIFVNMDVLLENISDDGCTGVIRTKYLTAMNAAWLISPLIMGYLVGADNNYQ
                     RIYYIGGLLMLPALFLIITRRRQFIDAKNRYRQQDAAITINQLLGHRDRRGIFLSSLT
                     LQFFYSLMSLYIPIYLHQHLGLSWSTLSIIFTFMLLPFVIIQMPAGLLADRFFGEKEM
                     LTIGNLIMIIAVVGIIFTKTANPFVWGFLLFFSRVGAALAESMHETYFFKKVGSRDMA
                     VINLYRQNKPIGRLIASLAAFITLSFLPLKSIFIVLFAGLVIGLIPIIYIRDTR"
     gene            10240..11163
                     /locus_tag="PKV49_02250"
     CDS             10240..11163
                     /locus_tag="PKV49_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HPL22464.1"
                     /translation="MPNHPTKIIYPADVPKAKRSLYNSNWQLLTKKTGRLLLLAGDQR
                     IEHLNSDFFGPGIDQADNDPEHFFQIADKMPIGAAAVHYGLASWYGQHYRRTAYVIKL
                     NGKTNLLPGDPISRCLAGVGQAIALAKQAGFRLAGVGYTIYLGSQHEGQMMAEASEII
                     RQAHAQGLLAIVWIYPKGVNVKNENDAEIIAGAAGVAVSLGADFVKLKAPKPLTVKNI
                     QRIVQAAGKTKVVFAGGSSRTAGQVFKDIQVQLAAGAAGVAIGRNIHQRSLTEASNWL
                     RGLAAMIYEQKTAEQVLSRLKINKPSRHKIK"
     gene            11189..11500
                     /gene="raiA"
                     /locus_tag="PKV49_02255"
     CDS             11189..11500
                     /gene="raiA"
                     /locus_tag="PKV49_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPL22465.1"
                     /translation="MQANIKAHGFELTLAITNYIKKKINMLDKYLGDIQPIACDVQVE
                     LMSRRRTKGDIFRAEINLQLPGELLRIEKTEPDLYKAIEKVKDHLAQMIIRYKEKKSG
                     R"
     gene            11617..14361
                     /gene="secA"
                     /locus_tag="PKV49_02260"
     CDS             11617..14361
                     /gene="secA"
                     /locus_tag="PKV49_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPL22466.1"
                     /translation="MSFLQKIFGDPNERIIKGLQPLIDQINNLEPTISQYTDEDLVQK
                     TTEFKSNYQQALQQSGSNGAQDYLDSILPEAFAMMREAARRTIGQRHFDVQLIGGIIL
                     HRGQIAEMKTGEGKTLVATLPLYLNALTGKGAHLVTVNDYLARIGAGWMGQVYHRLGL
                     TTAVIIHEQALKYDPDYEDSTQYDERLRHFRPISRQEAYNCDVLYGTNNEFGFDYLRD
                     NMVANQNQMVQRPLHYAIVDEVDSILIDEARTPLIISSAGEESTDKYYKFAELVARLD
                     EATDYNVDEKMRSVTLTEAGMNRMENFLGVENIYVEGGVREVMHIEQALKARVLFHKD
                     KEYVVKDGEVIIVDEFTGRLMYGRRYSEGLHQAIEAKEGVEIQKESRTLATITFQNFF
                     RMYEKLAGMTGTALTEAEEFSKIYNLETVVIPTNKPTVRRDFNDLIYATEAGKFQAVA
                     NDIKERHAKGQPVLVGTISIEKSEALADLLARQGVSAEILNAKYHEKEAQIISQAGRP
                     GAVTIATNMAGRGVDIILGGNPPDSAEQAKVLESGGLHVIGTERHEARRIDNQLRGRA
                     GRQGDPGSSQFYISTEDDLMRIFGGDRLKRMMSTLKLPEDMPIENSLVSKSIESAQKR
                     VEGNNFDLRKHLVEYDDVINKHRESIYRRRHQILQLSETEPSTETDQSPTTPLSGLIL
                     EMMDAEIEQVVSFHTQGENSEDWNIQEISQVMSTIIDIRPVELASQLIKLGESSTAKT
                     DKIAYRDALVKHLFDLVHQAYNELLVLAKDLNLDWLAIEKSVLLRSIDGLWIDHLEAM
                     DYMRKGIGLRGYGQRDPLVEYKKEAYRLFNELNSLIQKQVVYSIFKIVDFSRSNQQAI
                     GQMIAGADTPKNLVFKSGDETNAAPVAQKLKDSAGHKIGRNDLCPCGSGKKYKKCCGK
                     "
     gene            14776..15243
                     /locus_tag="PKV49_02265"
     CDS             14776..15243
                     /locus_tag="PKV49_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HPL22467.1"
                     /translation="MKKLMTLCVVEQGSKLLLGMKKRGFGAGRWNGFGGKVGIGETIE
                     AAAHRELLEEAGIVTKTPEKVGVIEFEFQHNGEILEVHIFKVVDFSGRPTESEEMKPQ
                     WFSVEQIPYNEMWPDDIYWLPLFLAGKKFVGKFLFGEGDQILEKELIEVDSLV"
     gene            15362..15664
                     /locus_tag="PKV49_02270"
     CDS             15362..15664
                     /locus_tag="PKV49_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPL22468.1"
                     /translation="MSKKLYVGGLSYSLTSEALKEEFAKAGTVDSAIVIIDKMSGRSK
                     GFGFVEMATEEEAQKAIDMFNGQEIAGRRVTVNEARPLTERPERTNNWDNRSTRSF"
     gene            15812..17875
                     /gene="secD"
                     /locus_tag="PKV49_02275"
     CDS             15812..17875
                     /gene="secD"
                     /locus_tag="PKV49_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPL22469.1"
                     /translation="MAHAEKRISAKQKSRAWQFFGLLVVLVVASLLVNLGPQYNNFIT
                     KHNWPLPKVKEVPFRLGLDLSGGTSLTYDADLSAVPVNDRMNAIQGARDVVERRVNMF
                     GVSEPNVQINKTTQGKYQISVELAGINDVKQAIKMIGETPLLQFKEVNDQRKELTDQE
                     KKDLDDYNKKAETKAGDVLGKVLSGGNFAELAKSLSEDDDTKAAGGSLGWLSSKEDPE
                     LIAIAEKLNKGQVNTNLQKTSAGYEIVKLEDKRPKTQDGQPVVEVKAAHLLVCYNGAV
                     SCESGLDKQQALDKIKKLKEQATVQNFTDLVKQNSTEPGARDSGGELGWFGRDTMDPQ
                     FEEAAFAMKKGEISEVVESQFGYHLLYKQDERAVIDYKLSHILIKTRSAVDILGNIDE
                     WKTTELTGKYLKRATVQFNPQDGSPEVALEFDSEGGKIFEDITGRNIDKPVAIFLDGY
                     IISQPNVSQKITGGQAVISGRFSQIEAKTLAQRLNAGALPVPISLVNQQTIGASLGQS
                     SVDNSLQAGLIGLALVALFMIIFYRLPGLLSVISLSIYGSLLLAIFKIWPVTLTLSGI
                     AGFILSIGMAVDANILIFERTKEELRAGKPLSNSINEGFSRAWPSIRDGNYTTLITCF
                     ILYQFLTGPIKGFAITLALGVLVSMFSAIVVTRNLLRLMITPRLAEKTWLFGVSRRQC
                     SGEDK"
     gene            17898..18815
                     /gene="secF"
                     /locus_tag="PKV49_02280"
     CDS             17898..18815
                     /gene="secF"
                     /locus_tag="PKV49_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPL22470.1"
                     /translation="MYQIIAKSKYWLIFSAIVIALAIAAIAVWGLHFGIDFTGGSVLE
                     ARYAQDRPTVDKIQDSLDPLGLDNLIVQPVGDNEIRLRFQEVDEAKHQEILNKLGELS
                     PQVDGQPGFEELRYDSIEPSVGRDLRSRALTAIFLVLVAIIIYVAWAFRKVSQPVSSW
                     KYGVCGVVALFHDVLIMVSFMALLGHYYGYQVDITSVAAVLTILGYSINDTIVVFDRT
                     RENLLRTRQSFAETVNISLNQTLTRSIYTSVTVMLTLVAIVLFGGASIRDFALILLIG
                     SFFGVYSSIFIASPLLVVWNDWSNRRKAK"
     gene            18876..20123
                     /locus_tag="PKV49_02285"
     CDS             18876..20123
                     /locus_tag="PKV49_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPL22471.1"
                     /translation="MRQSQLFTHSLKELPKDETSFNAQTLMRAGFIDKSSAGVYTYLP
                     LGLRVIQKIQTIVRQEMASLGAQEVLMPSLTTEEPWKQTGRLTSFDVLFKTTGADKKS
                     YVLAPTHEELVTPLVKKFVQSYRDLPLGVYQIQTKFRNELRAKSGLLRGREFLMKDLY
                     SFHLDQDDLNAYYLQAQKAYFQIFKRCGLLDQTYLTFASGGAFCQYSHEFQAVTEAGE
                     DIIHICEDCNLAINKEIISEQSVCPNCGRKDLLERKAIEIGNIFKLGVRFSKAFDFMV
                     TGSDGAKQPVLMGCYGLGITRLMGAIVEVNHDNQGLIWPKEIAPFKAHLIDLTTGDSG
                     HNQAEKLYDLLVRLGMEVLLDDRRHLPAGTKFADADLIGCPYRLVISDKTLSQKSFEI
                     KRRQDTAGRLVAIDQVSAYFGSN"
     gene            20132..>20895
                     /locus_tag="PKV49_02290"
     CDS             20132..>20895
                     /locus_tag="PKV49_02290"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPL22472.1"
                     /translation="MFERFRQKLTRNLAIDLGTCKTMLSEPQSGLFLTEPTIVAVNKK
                     TDQILAVGSAAKAMIGKNPSHIEIIKPLVDGVVSDFEVTEKLLKYFIDQTQESNSFLT
                     PKPAVVIGIPLDITEVEKKAVEDAAKSAGARQVFLVEEPMLSAIGAGLPIMEATATML
                     VDIGGGTTEIAVLALGGCVAWKSLRLAGNAFNNEIIQYIREEFSLLIGEQLAEEIKIK
                     IGSATPQPEELTMAIRGRDLINGLPKEIIINDEHVR"
BASE COUNT         6111 a         3730 c         4954 g         6100 t
ORIGIN      
        1 cggcagtagt taagccaaga attgggctta ccaaaaaagc attagctaaa actaggccgt
       61 ctagaaagtt gtgtaaaaaa tcgccaacca gattcatggt tcctagtgga tgtggatgtt
      121 ggctagatgt tggaatatgg caatgacgcc aatgaattat tttttccaag acaaagaagc
      181 ctaacattcc gccagttaaa tataaccagg gcgtaagacc agttcggccc tcggtggctt
      241 ctggtagtag gtggaaaaaa ctgtcgccaa ttaatgaacc ggcggcgata ctgattagcc
      301 agaaattgat ccgggccatt ttgtcggttg accagcgtaa actaattatt ccaattaatg
      361 aagcactgga aattaccgtt aacgataaaa atagatacag atagagcata gagcgattaa
      421 gtggtttgac tatattatag ataaaaaaac agccgccaac aagcggctgt tttgaggatc
      481 gattgattta ttttaatatt agtcagtttt tattgggttt acaaaagatg attaaagttt
      541 tattttataa atagtacctt gtgactacta aaatcaaaaa ggaggatgtt ttgaataatg
      601 gtaacaagtc aaccgtgcat catctgattc cgcgctctcg gggcggtgac tcgtccaaag
      661 ataacatcaa aagagtgcca tggcgaatac atgatgcctg gcatactcta ttcaacaatt
      721 tactgccgga agaagtttgt gctcaagtga tcacaactcc agaggacctg tttaacacgg
      781 ccaataaaaa aatggcctgg aaggttttat ttggctgcca ttggcaggag gttcaatctg
      841 gatcggcgca agaaaaaata ttgggtcaga taatgaccaa ttggttaccc cgaaactaca
      901 ttgtttcttg ggataaaatt aatcaattaa tctgggataa ttgttgcatt aattgtccca
      961 gagtcagttg ttgtctgact agataccaga aatggccgct tgagcacaaa caggtatttt
     1021 attattgcta cctaccagtt aaaaaagctc tagtggcgtt gttgatttaa aattttcccc
     1081 ggtggtagcc cggggttttt ttgtcgattg tttggctagg cagatatttg ttataataaa
     1141 aattattaaa ttaatggata gtataagtct ttattatgaa aaaacgtaag aaaatttggt
     1201 ggattatttt aggagtggtt attgttatta ttagtttagt ggtttggcag gggcggaaga
     1261 aaaatcagcc gcaatatacg acggtggcgg ctgaaatcgg atcattaaaa caaaccgtta
     1321 gtttgactgg tacagttaaa ccagttaagg aattagccct taatttttta tcagccggtc
     1381 ggattaataa tttaccagtt aaaatcggcg accaggtaag cgccggacaa ttattggccg
     1441 aattagatta tggctcgcta ttgttgcgta aaagcgaagc cgaggccggc ttacaaattg
     1501 ctcaagctaa tctcaataaa ttattgtctg gggctagcca acaagccatt gctgttaatc
     1561 gatctagtgt tgagcaggcg attagtagtc agcgaatggc gcaaattgaa ttggataaag
     1621 ttaaaaaaac ggcggcggaa aatattaagc aagccgaaaa atcggttaac gatctttatt
     1681 caagtgccgc cgatacccca acagtccaag agcaggcagt gattacggcg caaactaact
     1741 tagataatgc tcaacgaacc ggtcagcaga atattaataa cagtcgtagt tccggtttaa
     1801 ttatctttgg cgataaaata gcggctgcca aaattgcttt ggataatatt aatactatct
     1861 taactgataa taatgctaaa tatgtgctaa gcgttaaaga tagctcatac ttacaacgaa
     1921 ccaaagaggc gcgcaatcta gctctagata attatgcggc ggttagccaa gcagtcagtg
     1981 cggccaaaaa cgcctccgat actcaaagtt ttattgttgc tgctaataaa gccaagcaat
     2041 ttttgaatca aactgcctta gccataaact atagctattc catgttggag gcgacaatta
     2101 ccagtaacaa ctttactcaa ggacagctgg ataactataa ggggattgtc agtaatcaga
     2161 gcagccaaat taatggagct attacagccg ccgagacagc ggcgcaaaat tatcataacg
     2221 ctcaatctaa tagcgaaaca actgtggcca gcgctcaaga tagtttagaa caagccagag
     2281 tggctctgga gaatgctaaa ttagcggcag ccaataattt atctaccgtg cggctggcca
     2341 gtgatcgcca aatatctact gctcaatcac agctagataa tgccaataag gctgttgatg
     2401 tggctaaagc tcagctagct agtattactg caccagcccg gtcggccgat attgctttgg
     2461 ctcaaggaca gatcagtcaa gcacaggcca atctagctag cgcgcaaaaa caaattgatg
     2521 atagccgttt gttttcgccg cttgatggcg tagtgactca agttaattat gaggttggcg
     2581 aacaattttc agctgctggt aaagctttaa taatggtttt agttaataat agttttgata
     2641 ttgaagttga tgtcactgaa gccgatatta ataaaattaa agttggtaat ttagtggaga
     2701 ttactttaga tgcttttggc gatgatacta tttttcatgg tcaggtttat tttattgagc
     2761 ccgcccagac agttattcag gatgtggttt attataaagt caagatcagt tttagtgatt
     2821 cacaggaagt aaaaagtctg accgaagcgc gacaaatttc gattaaggct ggcatgactg
     2881 ccaacacaac cattacgacc gatcagcgtg agaatattgt taaaatacca ggtcgcgcta
     2941 ttgttgagaa agataatgga gtaaaggtag tgcgaattct caaagacaat aagctacagg
     3001 aaattccagc cgccattggt ctacgtggtg acgaggggtt ggttgaagtt cttggtggcg
     3061 ttcagcctgg tgatcaggtg gttactttta ttaaataccc aaagaccgcc gctgataagc
     3121 aataacaaag ccatggttaa ttatcctttg attgaactac aacatctaga caaaatttat
     3181 caaagtggcg atgtggtcac taatgttttg aaagacatca atttcaccat taatcgcggc
     3241 gaatttattt caatcatggg cccatccggc tctggcaagt caaccttaat gcatatttta
     3301 ggcttgttag atcggccaac cagtggccaa tatttattta atcaccacaa tactactgaa
     3361 ttatctgacg atcaattagc ggctttgcgt aatcatgata ttggttttgt ttttcaggct
     3421 tttaatttgt taaccagaac cagtgtcttg gataatgtta agctaccgtt gctttattct
     3481 aaagacaagg aacgaatgga tgaacgagcc actgcggttt tagaaagcgt tggcctggga
     3541 catcggctac attattttac taaccagata tctggcggcg aaaagcagcg agtcgctatt
     3601 gcccgggcac tagttaacga tccagaggtt atttttgctg atgaaccgac tggcaatctt
     3661 gattctaagt ccggcaatca ggtaatgcag attttgcagg agcttaacca acaaggtaaa
     3721 acaattattt tggtgactca tgaaactttt accgcggagc atgctcagcg aattattcgg
     3781 attcgcgatg gccagctggt tagtgacgag ttggtgccac agcagcgttt agctagtcag
     3841 ggacaggttt taaaataaaa tggttatagt ggaagacatt agacaatcac tgattttagc
     3901 ttggcgggct ttaatgagca ataaagtccg cagtttttta accatgctgg gcatcgttat
     3961 cggcgttagc gcagtaatta ttatcatgtc ggtcggtgct ggcgctcagt ctttaatttt
     4021 aaatcagttg gagggattgg gtagtgattt gatcgctgtt ttgcccggtc agtccgatga
     4081 aaatggtccg cccagccaag ctttcggtat tacggttacc actttaaaat atagtgatgc
     4141 ccaggcgtta cagcaggcta agaatatagc caatgttcaa gcggttaatg cttattatca
     4201 ggtgccgatt aatgccagtt ggcggaacaa tgattacgat gtttctctaa acggggtaac
     4261 taccagttat ttagatgtag aaaatggaac tgtggagcag ggtagatttt ttaccgacga
     4321 agaattcaat tcggcggccc gagtggctgt attagggagt gcggtagccg atgaattatt
     4381 tggttcaact gatcctttag ggcaaaagat aaagattaag aatcagaccc tcgaggttat
     4441 cggagtaatg aaaagtcgag gcaaggtggg ctttcaggat tatgatgacc aggtttttgt
     4501 gccgcttatt tttgtccaaa agatgattgc cggtgttgat tatgtcagct ttattagaat
     4561 taaattaaag ccgggcgccg atattaacca ggccctagaa gaagctcgac aaactttgcg
     4621 tgaacagcac aacattgata actcagttga tgatgatttt tctgtccgta gttttcgcga
     4681 tgccgttgct atgattacta ccattactga tgctttaaaa tattttttgg cagctatggc
     4741 ggctttatca ttactggtgg ggggtattgg tatcatgaat atattgttag ttagcgtaac
     4801 cgaaagaacc agagagattg gtttgcgtaa agctatcgga gcaaccagta accagatttt
     4861 gcggcaattt ttattagaat cagtctgctt aactttaatt ggtggtcttt tgggtatcgc
     4921 tagcggtatc gccgtttctt atttagtggc ggtggtagct aatttattgg aatataaatg
     4981 gtcttttatt atttctttgt cagccttatt gctagccgta tttatctcta tagccattgg
     5041 ggttattttc ggtctttatc cggcgcggcg agccagtcag ctagacccga ttgaggcttt
     5101 gcgttacgaa taatatgcga ttacagacag ccctacaatt agccatccga tcattgcgct
     5161 taaatctttt gcgaaccagc ttgacagttc taggtattat cattggagta gcggccattg
     5221 tggttgtttt tgccgctggt gagggtatta aaagtttggt tgttggcgaa gtagccgctt
     5281 atggcacaga tatcattgaa actgaagcca agacaccaac caagaaaaat accacaccgt
     5341 cagctcaagt caccacgctc aagaccagcg atatggaggc gattgatcgg ctggagaatg
     5401 ttaagtatag ttatggcgct atctttggtc aacaaaaggt tagctggcaa agtcaaaata
     5461 aaagagtttt tttatttggt gtcagtgcaa cctacaccga tattgataag aaaagtcagc
     5521 cgattgaagg tcgttttttt acggccgaag aagatcgcgg tcaggcgatg gtggccgttc
     5581 ttggttataa attgcgtcaa gaattattta ataatcagcc agccgtcggc cagtggatta
     5641 agattggcaa tggcaagttt caagttattg gcgtgcttga ggagcggggt ggtgcggtta
     5701 gtatgattga ttttgataac actgtttatt taccattaaa aaccttgcag aaaagaattt
     5761 taggggtgga tcatgtccta tatttgatgc atcaacttaa tgacgtttct cgagccgatg
     5821 ataccgctga agaaataaaa ttattaatgc gcgaaagaca taatattact gacccagata
     5881 aggacgattt cttggtaaca actatggagg aattattaaa gaccctaaac acggtaacta
     5941 acacaattac ttggttgttg ttggcaattg tggttatttc tttaatcgtc ggtggagttg
     6001 gtattatgaa tattatgtat gtcaccgtat ccgagcgcac gccggagatt ggtctgcgca
     6061 aggctttggg agcgacttat cgggatattg tcagccaatt tttggtggaa tcaattttaa
     6121 ttaccatttg ggggtcactg attggcatta tttttggatt gatcatggct tggattttga
     6181 gtttggcggc taattatgta gggttagtct ggcattttac cataccctgg tcgggcgtta
     6241 tcacagctat tattttttct ttagcttgcg gcttgttatt tggtattaga ccggcacaaa
     6301 aagcggctca attggatccg gtggaggcgc tcagggccga ataattatag atagcttttg
     6361 agagtaataa agccgaaggc ggcaaagagt agaaagatta ctaaaacaat aatccgtctt
     6421 ggccagccag gttgggtggc tggtggtaat gttttccaat tgccgatatt aactaaagcg
     6481 atatggacaa acattgctag agcgttaaca atagccccgg cgacgatgag ttgttttggc
     6541 tcataaaaat taagcaggaa gagactaata ccaaaaacaa tttgccccca cagaaaacga
     6601 taatagattt tagatagatt tagctgttta tccgggttat tttgcaattt aagtaaagcg
     6661 gcattttctg acataatacg ggaagtggaa tccagaacac ctagctgagt ttgggcgagc
     6721 attaaaccta ataagaccag gaaggcgctg ccaactagtg gcgcggtcgc ttgaccaatt
     6781 aagcgtcctt cggcgaggat aaaatttaaa ccagacaaat tgccgctaag accataagtg
     6841 gtgctataag atagaatcat taatagagcc attgataaaa acccgagtaa ccaaaatact
     6901 aataaatgtt ctaaactgat acgcttccac cactgacgga aacgccgtag attggtttga
     6961 ttaacaacaa aatcagtgcc cgataaattg atagtttgag tttgattaat attaaataag
     7021 ccgctcagtt tttgagcata gcgacccatg ccgtagcctt tgtctttaat ataaatcgat
     7081 tgggatagat tgagattgcc gccggctccg gcataagcga aagcagctaa aaaggtgatt
     7141 aaactgacac cggatggtaa ccagcgataa ttatggccat aacctaataa gccttggaac
     7201 aaggctgacc aatcagtcgg tttagctaaa aatattacta atatcagcaa gcacggtata
     7261 cccaccagga gtattgtttt agttatcttc tctagcgttt tatagacact ttgattaaag
     7321 gtcagaacag ctccaattaa tagcaagatg ccaatcgcta gccagcgaaa attttgaatg
     7381 ccaagcaagt gaccaaagat ttgagcgctg gcagcggcaa tgccgggcag tgcccaaccg
     7441 ataaaagttg ataaaataaa ccagtagggt acccaatgcc ataatctggc caaaccgcta
     7501 aaaacgcttt cgcctttaac cagggcgtag cgttcaattt ccatattgat aaaatattgg
     7561 caagtcagtc ctagcaaagc gccccagata atgccgagac catagttagc agctaaatat
     7621 ggccacaaaa taacttcgcc cgaaccaagt ccaagagcca gaataataaa acttggcccg
     7681 agcattttga taaaagaaat aggttgcgga aaagatttag tcgtcataca ataagattgt
     7741 agcaaaaaaa cttttaatct gacaaataaa attgatttat gtcggaacca ttagctagcg
     7801 ccatgcgacc gaataattta gataattttg tcggccaagc cgaaattatc ggtccggact
     7861 cattcttgcg tcaggcaata attaaacaac acttaccatc attattattt tgggggccgc
     7921 caggttgcgg taagacaacc ttggccaaaa tattggctaa tagtttggag gctgacttta
     7981 tcgctttgtc agccgtaacc agtggtttga aagaattacg gcaagttatt gatcaagcca
     8041 ggggcaacca gattgccaat aagccgacta ttctcttagt tgacgaaatt catcgctgga
     8101 atcgcggcca acaggatgca ttattgccat atgtggaaga cggcacaatt attttgattg
     8161 gcgccaccac cgaaaaccct agtttttcag tcaatgccgc cttattgtcg cgttgccagg
     8221 tggttatttt tcagcaattg actgctgacg atattatcaa tcttttagaa agagccttaa
     8281 aagacaagca atgcggtttg ggaaaattaa atatcaaggc cgatccgtcg gctttagcca
     8341 gaatcgctca gattgccgat ggtgatgctc gattatccct taatctattg gaagcagccg
     8401 ctaaaatcag ttcggaaata aatttagaag ttgtcaataa aattagccga cagattgttt
     8461 ttagttataa tcaagccggt gatgaacgct atcatttgat atccgccctg cacaaaagct
     8521 tacgaggcaa cgatgctagc gcggctgttt attggttggt tagaatgtta gagtcgggtg
     8581 aggatccttt gtatatcgcc cgtcgtttag ttcgttttgc tagtgaagat attggactag
     8641 ctaacaatac agctttattg ttggcgacta gttgttttga agcttgccgg cagattggtt
     8701 tgccggagtg taaagttgtc ttaactcagg cagttattta tttagctaaa tcacctaaga
     8761 gtattgccgc ctaccaagct tatcagcgag cggctgctga tgttaatcag tttggcgctt
     8821 tgccggtgcc gttgcattta ctcaatgcga gcactaaatt ggccaaagaa atgggctatg
     8881 gcaagaatta taaatatacg ccgctggaaa gcgatgatgg tcagagctat tggccggaca
     8941 aattgccagc caagaattat ttggcttagt tagcgagtgt cacggatata gataatcggt
     9001 atcaaaccga tgactaatcc agcgaataaa acgataaata ttgattttag cggtaaaaaa
     9061 cttaaggtga taaaggcggc cagcgaggcg attagccggc caatcggttt gttttgtcga
     9121 taaagattaa taacagccat atcgcggctt ccaacttttt tgaaaaagta agtttcatgc
     9181 atactttcgg ccaaggcagc gccgacgcga ctaaaaaata atagaaaacc ccaaacaaat
     9241 gggttggcag tttttgtaaa aataatacca acaacagcaa tgatcattat taggttgccg
     9301 atagtcagca tttctttttc gccaaaaaaa cgatcggcta aaaggccggc cggcatttga
     9361 attataacga atggcagcaa catgaaagta aagataatac ttaatgtgga ccagctcagt
     9421 ccgaggtgtt ggtgtagata aatcggtata taaagactca ttaggctata aaagaattgt
     9481 aaagttaagg acgataaaaa aataccgcgc cgatcgcgat ggcccagtag ttgattaatc
     9541 gttatagcgg catcttgctg gcgatagcga tttttggcgt caataaactg acggcgccga
     9601 gtgattatta ggaataaggc cggcaacatt aaaagtccac cgatataata aattcgttga
     9661 taattattat cagcgccgac taagtagccc ataattaatg gtgaaatcaa ccaagcagca
     9721 ttcatggctg tcagatattt ggtgcggata actccagtgc agccatcgtc agaaatattt
     9781 tctaataaga catccatgtt aacaaaaata aaaacaatgg atagataacg aacaataaat
     9841 gaaactaaaa tcagccaggt ggttgtttct gaagatataa taaagttggc ggccacagct
     9901 aagaagtaag taataatcat caaccggtaa gtagaatagc ggcgggctaa acgaggatat
     9961 ataaatgaga tggcgatcgt gataattgtt actagcgacc agaatagacc aatatattct
    10021 agtggtaaaa attgattcaa gaaagacgat tggacatagc caaccatggc gtcaattaag
    10081 gcagttatga agaccaatcc gtaaatcaga tagaaggttg atttggtttt ttgcataaaa
    10141 taaaaagtct gttaaaccgg aatagcttta ttatatagaa aattgtcgag aaagccaaat
    10201 cttgctataa ttaaattgtc aaaccttaaa tcattattta tgcccaatca tcccacgaaa
    10261 ataatctacc cggctgatgt gcctaaggcc aagcgtagtc tttataatag caattggcag
    10321 ctattaacca agaagactgg ccgcttgtta ttattagccg gtgatcagcg aattgagcat
    10381 ctcaatagtg atttttttgg ccctggcatt gaccaagccg acaatgatcc agagcatttt
    10441 tttcagattg ccgataagat gccgattggc gcagcagccg tccattatgg tttggctagt
    10501 tggtatggcc agcattaccg ccggaccgct tatgttatca agcttaacgg caagactaat
    10561 ttattacctg gtgatccgat tagtcgttgc ttggccggtg tcggtcaagc tattgcttta
    10621 gccaaacagg ccggttttcg attggctggc gtgggttata ctatttattt gggtagtcaa
    10681 catgaagggc agatgatggc cgaagcgtca gagattattc gtcaggcgca tgctcaaggt
    10741 ttgctagcca ttgtttggat ataccctaag ggggtaaatg ttaaaaacga aaatgatgcg
    10801 gaaataattg ccggcgcggc tggggtggcg gttagtctcg gagcagattt tgttaaatta
    10861 aaggcgccta agccactgac agttaaaaat attcaacgca tcgttcaggc ggctggaaaa
    10921 actaaggttg tttttgccgg cgggtccagt cgaacggctg gtcaagtttt taaagatatt
    10981 caagttcaat tagcggccgg cgcggccggt gtggcgattg gccgtaatat ccatcagcgc
    11041 tcgttgacgg aagccagtaa ttggctgcgt ggattggcgg cgatgattta tgaacaaaaa
    11101 acagctgagc aggttttaag ccggttaaaa attaataagc ccagccggca caagattaag
    11161 taacttttat ttttaaatat atacgcctat gcaagccaac attaaagctc atggttttga
    11221 attgacactg gccataacca attatattaa aaaaaagatc aacatgttgg ataagtattt
    11281 gggcgatatt cagccgattg cttgtgatgt gcaagttgaa ttaatgagtc ggcgtcgcac
    11341 taagggtgat atatttagag cggagataaa tttacaattg ccaggggagt tattgcggat
    11401 tgaaaaaacc gaacccgatc tttataaggc gattgaaaaa gttaaagatc atttggcgca
    11461 gatgattatc agatataaag agaagaaaag cggtcgttag agggctaatt taggttgaaa
    11521 aaagcaaggc gttggccttg ctttttgctt tatttagact ataatggaaa aaatgagtta
    11581 gtgccgttta agcaggcgat ttaattaata aatattatga gttttttaca aaagatattt
    11641 ggcgacccga acgaacggat aattaagggt ttgcagccgt tgattgacca aataaataat
    11701 ttggagccga ctattagtca atacactgat gaagatttag ttcaaaaaac cactgaattc
    11761 aagagcaact accagcaggc gttgcaacaa tctggtagca acggggccca ggattattta
    11821 gatagtattt taccagaagc atttgctatg atgcgtgaag cggctcggcg aacaattggc
    11881 cagcgtcatt tcgatgtcca attaatcggc ggcattatcc ttcatcgcgg acagattgct
    11941 gaaatgaaaa ccggtgaagg taaaacttta gtggcgactt tgccgttata cttaaacgct
    12001 ttaaccggta aaggggcgca tctggttacg gttaatgatt atttggctag aattggtgct
    12061 ggttggatgg ggcaagttta tcatcgtctc ggtttaacaa cagcagtcat tattcatgaa
    12121 caggccctca agtatgatcc agattatgaa gatagcactc aatacgatga gcgcttgcgc
    12181 catttccggc caatcagtcg tcaggaggcc tataattgtg atgttcttta tggcactaat
    12241 aatgaattcg gttttgatta tttgcgcgac aatatggtgg ccaatcagaa tcaaatggtt
    12301 caacggccgc tgcattatgc tattgttgat gaagttgatt ctattttaat tgatgaagcc
    12361 cgcacgccat taattatttc tagcgccggc gaagaatcta ccgataaata ttataaattt
    12421 gccgaattgg tcgccagact ggatgaagcc actgattaca atgttgatga aaaaatgcga
    12481 tcggtaacat tgactgaggc tggcatgaat cggatggaaa attttttggg cgttgaaaat
    12541 atttatgttg agggtggtgt ccgcgaagtc atgcatattg aacaggcgct aaaagcgcgc
    12601 gtcttgtttc ataaagacaa ggaatatgtg gttaaagacg gtgaggtgat tatcgttgat
    12661 gagtttaccg gccgactgat gtatggtcgt cgttatagtg agggtctaca tcaagccatt
    12721 gaggctaaag agggggtgga aattcagaaa gagagccgga ccctggccac gattactttt
    12781 cagaattttt ttcgcatgta tgaaaagtta gccggtatga ccggtacagc tttaaccgaa
    12841 gccgaggaat tttccaagat ttataatcta gaaacggttg ttattccaac taataagcca
    12901 actgtccgcc gcgattttaa tgatttaatt tacgccacgg aagccggtaa atttcaagcg
    12961 gtggctaacg atatcaaaga gcgtcatgcc aaaggacagc cagttttggt cggcactatt
    13021 tcgattgaaa aaagtgaagc tctagccgac ttattggctc ggcaaggtgt gtcggcggaa
    13081 attttgaacg ccaagtatca cgagaaagag gcgcaaatta tttctcaagc cggtcggcct
    13141 ggagcggtaa ctattgccac caatatggct ggtcgcggcg ttgatattat tttaggcggc
    13201 aatccgcccg atagcgccga gcaagccaaa gttttagagt ctggcggtct acatgttatc
    13261 gggactgagc gtcatgaggc gcggcggatt gataatcaat tgcgcggtcg tgccggtcgg
    13321 caaggcgatc ccggctctag ccaattctat atttcaaccg aggatgatct gatgagaatt
    13381 tttggcggtg atcgtctgaa aaggatgatg tcaactttga aattgccgga agatatgccg
    13441 attgaaaaca gtctagtttc taaatcaatt gaatcggccc aaaaacgagt tgagggtaat
    13501 aattttgatc tgcggaaaca tttagttgag tatgatgatg ttattaataa acaccgggaa
    13561 agtatttatc gccggcggca ccaaattttg caattaagtg aaactgaacc aagtacggag
    13621 acggaccagt cgccaactac gccacttagc ggcttgattt tagaaatgat ggatgctgag
    13681 attgagcagg ttgttagctt tcatactcaa ggtgaaaata gtgaagattg gaatattcag
    13741 gaaattagtc aagtaatgag tacgattatt gacattcggc cggtcgagtt ggctagtcag
    13801 ttgattaaac tgggcgagag ctcaactgcc aaaaccgaca aaattgctta tcgcgacgcg
    13861 ctggttaaac atctgtttga tttagtgcat caggcttata acgaattatt agtgttggcc
    13921 aaagacttaa atttagattg gctggcgatt gaaaaatccg ttttattgcg atcaattgac
    13981 ggactatgga ttgatcattt ggaggcgatg gattatatgc gcaaaggtat tgggctaagg
    14041 ggttatggcc aacgcgatcc attagttgag tataaaaaag aggcttatcg attatttaat
    14101 gaactcaata gtctgattca gaaacaggtg gtttattcta tctttaagat tgttgatttt
    14161 agtcgttcca atcaacaagc catcggtcaa atgattgccg gagcggatac tcccaagaat
    14221 ttagttttta agtcgggcga tgaaaccaac gcggcaccag tggcgcaaaa attaaaagat
    14281 agcgcggggc ataaaattgg tcgcaacgat ctttgcccat gtggcagcgg aaaaaagtat
    14341 aagaagtgct gcggaaaata aaaagccgca agaggtttta gagatgtttt gtacacggtg
    14401 tttcgtgtta gaacaataat ggtttttcgt tcgtcactac taaaccctct aagtcttgcg
    14461 gcaacctatc cgtgtcactc cttttaggag aagcagaaag ttcggctgct tctttctgcg
    14521 gatagggtaa gcatagttaa tttatataga gatgtcaata aatctatcta tggaaagatt
    14581 ttcaagaacc gagttagggg agagatgtgt ttgttccatt gtcggcagca actattattt
    14641 aggattatct tcagcttggg ggagcaccaa ccgaagttgt taattatata gtcaatgatg
    14701 gtttggatat gaatgatgct gctttcaaag ccggcctgac cgccttgatt catattaatt
    14761 gttaatgcgt ttgctatgaa aaagctcatg acgctgtgcg tagttgaaca ggggtcgaag
    14821 ctgttgctgg gcatgaaaaa gcgaggtttt ggtgctggcc gttggaatgg ttttggcggc
    14881 aaggttggta ttggtgaaac gattgaagcg gcggctcacc gcgaactttt ggaagaagcc
    14941 ggtattgtta ctaaaacgcc agaaaaagtc ggcgttattg aatttgaatt tcaacataat
    15001 ggagaaattc tagaggttca tatttttaaa gttgttgatt tctcgggtcg accgaccgag
    15061 agcgaagaaa tgaaaccgca gtggtttagt gttgagcaga ttccttataa tgaaatgtgg
    15121 cctgacgata tttattggtt gccattgttt ttggccggca aaaagttcgt tggcaaattt
    15181 ttgtttggcg aaggtgatca gattttggaa aaagaattaa ttgaagtgga cagcttggtt
    15241 tgacttttac ctattttcct gttatattga tcacgtaagt ttttctttgt gtgatctcta
    15301 aaagtcgccg agaaataggt cgacttactt ttatttaaaa cgtataagtt cgtaagtttg
    15361 tatgtccaaa aaattatacg ttggtggatt gtcttacagc ctcaccagcg aagccttgaa
    15421 agaggaattc gctaaggccg ggaccgtcga ttcagctatt gtcattattg acaagatgtc
    15481 tggtcgttcc aaaggttttg gttttgtgga gatggctact gaggaagaag ctcaaaaggc
    15541 aattgatatg ttcaatggcc aggaaatcgc tggccgccgc gtgactgtca atgaggctcg
    15601 ccctttgact gaacgcccag aacgaaccaa caattgggac aatcgttcaa ctcgcagttt
    15661 ctaattgcaa gttagcctat tgtttataga gacgccgccc ttgattgggg cggcgttttt
    15721 agtcgttgat ttttttagca aagtggacta tgatgaagta aattaacctt agttaattta
    15781 attttggcgt tttaaatttt aaaaattgtt tatggctcac gcagaaaaga gaatatcagc
    15841 caaacagaaa agccgcgctt ggcagttttt cgggctattg gtggttttgg tagtagctag
    15901 tttactggtt aacctaggac cgcagtataa taattttatt accaagcata attggccatt
    15961 gccaaaagtt aaagaagtgc catttcgttt ggggttggat ttgtctggcg gtactagttt
    16021 gacctatgac gctgatttgt ctgccgttcc ggtcaatgat cgcatgaacg ccattcaggg
    16081 ggcgcgcgat gtggtggagc gccgggttaa tatgttcggc gttagtgaac ccaatgttca
    16141 gattaataaa actactcaag gcaaatacca gatttcggtg gaattggccg gtattaacga
    16201 tgttaagcag gcgattaaga tgattggcga aactccgcta ttgcaattta aagaagttaa
    16261 tgatcagcgt aaagaattaa ctgatcaaga aaagaaagat cttgatgatt acaataaaaa
    16321 agccgaaacc aaggctggcg atgtcttggg taaggttttg tccggtggta attttgccga
    16381 attagctaag tctttaagcg aagatgatga taccaaggca gcgggtggat ctttgggttg
    16441 gctaagcagc aaagaagatc cggagttaat tgctattgct gaaaaattaa acaagggtca
    16501 agtcaatact aatttacaaa aaacatcagc tggttatgaa atcgttaagt tagaagataa
    16561 gcgccccaaa actcaggatg gccagccagt agttgaggtc aaggcggctc atttattagt
    16621 ttgttataac ggcgctgtta gctgtgaaag cggtttggat aagcagcaag ccttagacaa
    16681 aattaaaaag ttaaaagagc aagctactgt ccaaaatttt accgatttag ttaaacagaa
    16741 ttcgactgag ccgggagctc gtgacagtgg cggtgagctt ggttggtttg gtcgcgacac
    16801 catggatccg cagtttgaag aagcggcttt tgctatgaaa aaaggagaga tttctgaagt
    16861 cgtggaaagt caattcggtt atcatttgct ttataagcaa gacgagcgcg cggtaattga
    16921 ttataaatta agccacatct tgattaagac tcggtcggca gtggatattc ttggcaatat
    16981 tgatgaatgg aaaaccactg aattaactgg taaatatttg aagcgagcga cggttcaatt
    17041 taatccgcaa gatggttcgc cggaagtagc gctagaattt gatagtgaag gtggtaaaat
    17101 ttttgaagac attactgggc gcaatattga taagccagtg gctatttttt tggacggtta
    17161 tattattagc cagcctaatg tcagccagaa gattactggc ggtcaagcag taatctccgg
    17221 tcgttttagt caaattgagg cgaaaacatt agcgcaacgt ttgaatgccg gagccttgcc
    17281 agtaccgatc agcttggtta atcaacaaac cattggcgct agccttggcc aatcatcggt
    17341 tgataatagt ttgcaggccg gtttgatcgg cttagcctta gtagcgctat ttatgattat
    17401 tttttatcgt ttgcccggct tgttgtcagt tatatctctg tcaatttatg gttcattgct
    17461 gttggccatc ttcaagattt ggcccgtcac tttaacttta tcaggtatcg ccggttttat
    17521 tttgtctatc ggtatggcag ttgatgctaa tatccttatt tttgagcgta ccaaggaaga
    17581 gctgcgcgct ggcaaaccat taagcaattc cattaatgaa ggtttttcac gcgcttggcc
    17641 ctcaattcgt gatggtaatt atacaacctt aattacttgt tttattctat atcagttttt
    17701 aaccggtcca attaaaggat ttgccattac tttagcgctg ggtgttttag tgtcaatgtt
    17761 tagtgctatt gtggttactc gcaacctttt gcgtttaatg attacaccgc gtttagctga
    17821 aaaaacttgg ctatttgggg ttagtcggcg acaatgttcc ggtgaagata aataattaat
    17881 tgctattatt catccatatg tatcagatta ttgcaaaaag taaatattgg ttaatatttt
    17941 cagctatcgt catcgcttta gcgattgcag cgattgcggt gtggggttta cattttggta
    18001 ttgattttac tggcggcagt gttttagaag ctcgctatgc ccaagatcgg ccgacggttg
    18061 ataaaattca agatagcctt gatccattgg ggctagataa tttaattgtc cagccagttg
    18121 gtgataatga aatcagattg cgctttcaag aggttgatga agctaaacat caagaaatat
    18181 taaacaagct gggagagcta tcaccgcaag ttgatggcca gccaggcttt gaggaattac
    18241 gttatgactc gattgagcca tcggttggcc gtgatttgcg tagtcgagct ttgaccgcca
    18301 tttttttggt tttagtggca attattattt atgtagcttg ggctttccgc aaagtttctc
    18361 aaccggttag ttcttggaaa tatggtgttt gtggcgtggt ggctcttttt catgatgttt
    18421 taatcatggt tagttttatg gccttgctgg ggcattatta tggttaccaa gtagatataa
    18481 cttcggttgc ggccgtgctg actattttgg gttatagtat taatgatacg attgtagtat
    18541 ttgaccgaac cagagaaaat ttattacgca ctcgacagag ctttgccgaa acggtcaata
    18601 ttagtttgaa tcagacatta actcgctcaa tctatacttc agtgacggta atgttgactt
    18661 tagtggcgat cgttttattt ggcggagcct caatccgtga ttttgcttta attctattga
    18721 tcggttcatt tttcggtgtc tatagttcaa tttttattgc ctcaccgcta ttagtggttt
    18781 ggaatgattg gagtaatcgt cgtaaagcta aataaagtcg catcttacct aagaccggtc
    18841 atggtcttac agctattaat tattttttat attttatgcg ccaaagccaa ctttttaccc
    18901 atagtcttaa ggagctgcct aaagacgaga ccagttttaa tgctcaaaca ttgatgcgag
    18961 ctggttttat tgataaaagt tcggctggtg tctatactta tttgccgctg ggtcttcgag
    19021 taattcagaa aattcagacg attgttcgtc aagaaatggc ttctttggga gcgcaagaag
    19081 tcttaatgcc cagtttgacg accgaagaac catggaaaca gactggtcga ttaactagtt
    19141 ttgatgtcct atttaagacc actggtgctg acaaaaagtc ttatgttttg gctccaactc
    19201 atgaagaatt ggttactcca ttagtcaaaa aatttgttca gtcttatcgc gacctgcctt
    19261 tgggggttta tcagatacag actaagtttc gcaatgaatt gcgggccaaa tcagggctat
    19321 tgcgcggtcg tgaattttta atgaaggact tgtattcttt tcatttggat caagacgatc
    19381 ttaatgccta ttatttgcag gcccaaaaag cctattttca gatttttaag cgctgcggct
    19441 tattggatca aacctatttg acctttgctt cgggtggggc tttttgtcag tattctcatg
    19501 agtttcaagc agtgaccgaa gccggtgaag atataattca tatttgcgaa gattgtaatt
    19561 tggccattaa taaagaaatc attagcgagc agtcggtttg tccgaattgt ggtcgcaagg
    19621 atttgttaga gcgcaaagcg attgaaattg gcaatatatt caaattaggt gttcgtttta
    19681 gcaaggcttt tgattttatg gttactggat cagatggcgc taaacaaccg gtgttaatgg
    19741 gctgttatgg cttaggcatt acgcgattaa tgggggcgat tgttgaagtt aatcatgata
    19801 atcaaggttt gatttggcca aaagaaattg cgccgtttaa ggcccatctt atcgatctaa
    19861 caactggcga tagcggtcat aatcaagctg aaaaattata tgatctttta gttagattgg
    19921 gcatggaagt attgttggat gatcggcgcc acttgccggc tggcaccaag ttcgccgatg
    19981 ccgatcttat tggctgtcct tatcgcttgg ttattagtga caaaacttta agtcagaaga
    20041 gttttgaaat taagcgccgt caagacactg ctggccgatt ggtggccata gatcaagtta
    20101 gcgcttattt tggcagtaat taagactagc catgtttgag agatttcggc aaaaacttac
    20161 ccgtaattta gctatcgatc ttggcacctg caagactatg cttagtgaac cgcagagcgg
    20221 tttattttta actgagccga ccattgtggc ggttaacaag aaaacggatc aaatattagc
    20281 tgtcggcagc gctgccaagg caatgattgg caaaaatcca tcgcatattg agattattaa
    20341 accattagtg gatggggttg tttctgattt tgaagtaact gaaaaattgt taaaatattt
    20401 tattgatcaa acgcaggaga gcaattcttt tttaactcct aagccggccg ttgtgattgg
    20461 tattccgttg gatattaccg aagtggaaaa aaaggcagta gaagatgcgg ctaagtcggc
    20521 tggagcccgc caagtttttt tagttgagga gccgatgcta tcggcgattg gcgctggctt
    20581 gccaatcatg gaggcgacgg ccactatgct agtagatatt ggcggtggaa ccaccgaaat
    20641 tgcggtctta gctctggggg gttgtgtggc ctggaagtcg ctgaggttgg ccggcaatgc
    20701 ttttaataat gaaataattc aatatatccg tgaggagttc agcttgttaa ttggcgaaca
    20761 attagctgaa gaaataaaga ttaaaatcgg ttcagccacg ccacagccag aggaattaac
    20821 aatggcgatt cgtggccgcg acttaatcaa tggtttgcct aaggaaatca ttattaatga
    20881 tgagcatgtc cgtga
//
LOCUS       DAOPAI010000013        16564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_3545_length_16564_cov_6.073112, whole genome shotgun sequence.
ACCESSION   DAOPAI010000013 DAOPAI010000000
VERSION     DAOPAI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16564
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3545_length_16564_cov_6.073112"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1313)
                     /locus_tag="PKV49_02295"
     CDS             complement(153..1313)
                     /locus_tag="PKV49_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPL22473.1"
                     /translation="MFLVINKKSKYSLAAFTLIELLVIIAIIALLSTLSVIALNNARA
                     KARDARRLSDVKQISTALELYYNSHGIYPNSPNPVGTPITNLCLSELGITSTCGTEVY
                     MKNIPADPAKDKHYLYAPLINNSSFNLIYDEETIADCPTDWVRVPGNTLYNTHSFCVM
                     KYEAKIKGIDNGNTAYSASYEPESRPSGTPWVNITQTNAKLECESIGAHLITEDEWLS
                     IVRNLELQASNWTGGAIGSGQITRGNSNSSAAMDGTSELDGVNKRTHILSNGEVIWDL
                     AGNVYDWTDATIMGKNQPSGVVSEAGWLEYTVLTNYGDIAPEKLLPMNNYNSNQGYGQ
                     IFSIGSSTNTINYGFRRGGYWRNNGARAGVLTLILNVPPNDYYKYIGFRCAL"
     gene            1429..2490
                     /gene="ychF"
                     /locus_tag="PKV49_02300"
     CDS             1429..2490
                     /gene="ychF"
                     /locus_tag="PKV49_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPL22474.1"
                     /translation="MALSIGIVGLPNVGKSTLFNALTKKQVDAANYPFCTIEPNIGIV
                     KVPDERVDQLSAMSHSAKTIYTTMEFVDIAGLVKGAHEGEGLGNQFLAHIRACDAICQ
                     VVRNFQDDNIVHVDGRINPESDQAIINLELIYADLATVNKRLDKVGREAKSGHKTAIQ
                     QTAILTKLRDYLDNGHSLRQFDFTADEQALIKELNLLTSKPIIYVINSSQPETEQLPA
                     WDGAVLPLNAKLEADIAALPENEQAEYIQELGLNQSGLNKLIAQAYQLLNLVTFLTTG
                     ADETRAWTVIKNTPAPLAAGVIHTDFIKGFVRAEVINWQDLLRAGSEVKAKELGLLRL
                     EGKDYLIKDGDVCNFLINN"
     gene            complement(2487..3653)
                     /locus_tag="PKV49_02305"
     CDS             complement(2487..3653)
                     /locus_tag="PKV49_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPL22475.1"
                     /translation="MNYFFILGSHPALSLTELAVKLSGRWQVVNDNLALASQVAELSA
                     DLLEYLGGTIKFGRVIGQFNQLNEAAAQVIAKQLLAVAPADKKICFGLSNYDRQPLSR
                     DLGIQIKRFIQDTGRSARWVISRAAALSSVVVEQNHLLTSGGEVVLWRQDNCWQWGVT
                     VAVQPFKSLSARDYGRPARDDQSGMLPPKLAQIMLNLAGFEPGDSVLDPFCGSGTILQ
                     EAALMKAGRIIGLDNSGVAVANSQINWQWLANRWPIEIDPEISLADARQASVQLANQR
                     IKRIVTEPYLGPQRGRRNFMVIAQELNQLYSQALVDWQKIIAPGGRVVMIWPVFIDGH
                     QKHLVQPKMAGWRKVKYSQLARQAWPKQYTLDGNLLYGRPGQVVWRAIVGLEKV"
     gene            3889..4413
                     /gene="rpsF"
                     /locus_tag="PKV49_02310"
     CDS             3889..4413
                     /gene="rpsF"
                     /locus_tag="PKV49_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPL22476.1"
                     /translation="MAKNKSSRLPRYELLYIVSNKFTAEETEPIAQKIENLITANDGT
                     IVYRENWGKRRMSYPIKGFLFGYYRLLECDLAADKIAEVERFLRLSSDILRHQIVKRQ
                     PRTVEEILAAKEEIAKREAKLQSDKTEAEAKKNNPEKEAATPASDSEEVAKKHDQDID
                     AKIDTLINEEPIVK"
     gene            4438..4890
                     /locus_tag="PKV49_02315"
     CDS             4438..4890
                     /locus_tag="PKV49_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPL22477.1"
                     /translation="MDLNKAMLIGNLTRDPEIRNTTTGQPVASFTVVTNYVWTDQTGQ
                     RQERAEFHNIVAWRRLADICRQYLYKGSKVYVEGRLQTRDWLAQDNTKRYRTELVADN
                     LIMLDRAGSASAPFSGPAAEQTDNNEPSLESLPDNPAEEEIKIENIPF"
     gene            4909..5127
                     /gene="rpsR"
                     /locus_tag="PKV49_02320"
     CDS             4909..5127
                     /gene="rpsR"
                     /locus_tag="PKV49_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPL22478.1"
                     /translation="MNSRNSNKPCYFCQNGIKEIDYKDTFLLRRYANALGKILPKHRT
                     GTCAKHQRQLTLAIKRARIMAMLMFVRR"
     gene            5137..5697
                     /gene="efp"
                     /locus_tag="PKV49_02325"
     CDS             5137..5697
                     /gene="efp"
                     /locus_tag="PKV49_02325"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPL22479.1"
                     /translation="MLSLNEIKLGKVITINGEPYSVIRADHHKMGRGGAVLKTKLRNL
                     ISGSVLERTWQGNDRADEADVEKRKATFMYTDKQFANFMDSADYEQFAINLEELGGKE
                     KFLKDGLEVTVLYFEGRPVAVELPVKAEYKVITAPPGVKGNTVGNVMKQIEIETGAQL
                     NAPLFINEGDIIRVNTDTEEYVERVN"
     gene            complement(5768..6820)
                     /gene="recA"
                     /locus_tag="PKV49_02330"
     CDS             complement(5768..6820)
                     /gene="recA"
                     /locus_tag="PKV49_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPL22480.1"
                     /translation="MANDKKNKKDKELASVDSGNLSEKQKAAEAAMSQIRQLYGEGSI
                     MKFGDVKNTNVDAVTTGCLSLDLAIGVGGVPRGRVIEIYGPESSGKTTLAQHIIAEVQ
                     KQGGIAAFIDAEHALDPEYAKKIGVNIDEMLISQPDTGEQALEILETLVRSNAVDVVV
                     IDSVAALVPKKEIEGDMGDLQMGSQARLMSQALRKLTGVIAKSKTIVIFINQLRMKIG
                     VMFGNPETTTGGNALKFYASVRIDIRKIAQIKQGEKMLGNRVKCKVVKNKVAAPFKSC
                     EFDIMYNEGISLAGDVLDTGVEMGTITKSGNSYSFGEIKLGVGRESAKTFLRQDKELM
                     KKIRKQVLIDLAAKQE"
     gene            complement(6877..8292)
                     /locus_tag="PKV49_02335"
     CDS             complement(6877..8292)
                     /locus_tag="PKV49_02335"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPL22481.1"
                     /translation="MKKKIKVWFLSAEVAPFAKTGGLGDVAGALPKALAKLGVDVRIC
                     LPLYGLIDRRAYQIKPLKTTAIVNWSTGAEKIRIYQTRLPGSQVPVYLLDHLFLNTAT
                     IYLQPNDPDDKPNRKIDDVRKFSFFTKAALAAAEALKFQPEIIHCHDWHTALVQHLLH
                     QWPSDFYEVSRVVYTIHNLANQGWAGHNIVRWAGLKNWQGDLNFMAEGIKTADWVTTV
                     SPSYAQEILSRPLDAGLGRYLRQRRRNLSGIINGIDVVEYNPAQDKNIVKKFSAQTLE
                     RKKINKLALQKELGLKQDANIPLAGLISRLVWQKGLELFDQQMIKRNCQWVFLGTGEP
                     RYEKQLAALAAKYPDRISAQIKFNDRLAHQIYASADIFLVPSRFEPCGLTQMMAMRYG
                     AVPIVRSTGGLKDTVNKNNGFLFNKLTKTEFYSTIDKALDEYGQRPAIWRQRQLVGMN
                     QDWSWNEPAKEYLRLYRRLLQ"
     gene            complement(8299..9165)
                     /locus_tag="PKV49_02340"
     CDS             complement(8299..9165)
                     /locus_tag="PKV49_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPL22482.1"
                     /translation="MLIDLQIHSIYSDGYLTPTAVAKFLAQQGVKVASLTDHNTLAGW
                     PEFYQACQKLKIKPIPGIELYTRLNNQQINLLWYNLPIGDQQVAKLLLTSQRHWKVRL
                     KQHLQKLVKAKLLVKDSHRVVEKFTNFIPTNGLLDALLLVPGNREIIVERLDDSRFRE
                     EEAIGLLFGNKKIGRLRLSCIDINRVIKLRRELGGQLVFCHPAKGRSIKRSLVHRLKN
                     LGIDGIEVLSPHHSLGAIMYAQDLAKRYNLIITAGSDFHRFEEVSCGLKSAYDYWQAD
                     SQDLQRINEIIG"
     gene            complement(9170..10180)
                     /locus_tag="PKV49_02345"
     CDS             complement(9170..10180)
                     /locus_tag="PKV49_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPL22483.1"
                     /translation="MASIQDILDPIKVKQLFNQRKAEIDPAITEIVVEKVKVIKSNFH
                     HEYYSLVIAYDLIAKQGGADIKLSIYCSAHSSESRRQTWDNLRLLWDKQIANKQFLTN
                     RPLFYDPDCRGLFYTGLPGRNLDQYFKAGQLDQLNDYLQSIAGWLARLHKLTKGLRDW
                     PDSRQQLIGQVVPGRQRALNKIRQNCPQEYSRFVQLYDILERREAEHWPSRNNLTMIH
                     GDLHPENIIVNQKRAAIIDWADASLGDPARDLGAFVQQLAFMGQRHLPDIAYWQQAQK
                     FFLEAYQTIVGEVMAADWSERLETYYNFAAFRTAVYFVTKSGPEPQRASGLLTEVEFN
                     LI"
     gene            complement(10185..11333)
                     /locus_tag="PKV49_02350"
     CDS             complement(10185..11333)
                     /locus_tag="PKV49_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22484.1"
                     /translation="MRWVNVLHFYQPPLSRPEIVAEAVRLSYRRVTKALLKSPNSRVT
                     VNFAGCLLEQLLLLNQHELIADWQRLVASGQVELTGSAYWHALLPKISGEELASQVAA
                     QEKILARLFKIARPAGFFAPELAYSPELIQWLAQRGYHYAIVDEISIDGRLDTPKQMF
                     YRDQVSGLVLAVRQRDWSKKYPPEILTQGIDHCPQNLFTVTDGELYGLRHRDYSGKLE
                     RCWRNPKIKTETVSQAIASQDNLTAISLVAASWESWPDEIAAKQPFAVWDSRANHLQK
                     MLWSLADLAQQAAQRFAGDSNQKWVARHLHQGLSSCAWWWASGRDFALFGSAAWGPEE
                     IIRGAEQLVKSVRSIDNPASRPWKQQADKIFCSLQTRIWQKHWQQWKR"
     gene            complement(11338..12798)
                     /locus_tag="PKV49_02355"
     CDS             complement(11338..12798)
                     /locus_tag="PKV49_02355"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPL22485.1"
                     /translation="MSRILKIASVASEVAPFSKTGGLADVARSLPKAVNRLGHQVIVI
                     TPLYNRVINKQKYKLKEIFQDIQLMVGGKTIAVNYWQGYLMEGLPVYFVENKEYFGKK
                     QDIYGLANDNARFYLFDVAVLRLLELLEFKPDIIHCHDWQTGLIPQLLNSQFKKSAVL
                     RGARTVFTIHNLIFQFGHNWWQTPADKKDFGTKKLPDLTNPDLEYVNFAKRAILQANI
                     INTVSEQYREEIMTRHFGQDLHRILRNRIDRLFGIINGIDHFDYNPSNDDSLFTKYDW
                     RSAIVGKANNKAYLQEKLGLQVDRDMPLLCATSRITFQKGFSLIMEIMGWLLKRDVQI
                     VILGDGDSQYINQLKAWRKKYPRRLAVLSYKKNLKLESLIYAASDCLLLPSHHEPCGI
                     NQLIAMRYGCVPIVRNVGGLNDTVNNFNPRTNRGTGFVFNNFTAIALYTAIIRALENY
                     HHQSSWAGLLKRGMKTASSWKIPAKKYINLYRRALK"
     gene            complement(12804..13316)
                     /gene="ruvC"
                     /locus_tag="PKV49_02360"
     CDS             complement(12804..13316)
                     /gene="ruvC"
                     /locus_tag="PKV49_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPL22486.1"
                     /translation="MAKAVKIIIGFDPGLADTGFGVIRLDGPNTDCLEYGSLKTAAKV
                     PLPERLASLYQQVKDLLAKYQPDIVGIEQLFFCRNVTTAFQVGQARGVVMLAIGQQAI
                     PCLEFTPLQIKQAVAGYGQADKRQVQKMVQLILRLKEIPRPDDAADGLAAALCAAQSQ
                     ATLLASSDKH"
     gene            complement(13316..14047)
                     /locus_tag="PKV49_02365"
     CDS             complement(13316..14047)
                     /locus_tag="PKV49_02365"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPL22487.1"
                     /translation="MSGHSKWATTKRQKAVVDAKRGAVFTKIGNLISIAARDKGGDPA
                     TNFSLRMAIDKARQANMPKDNIERAIKRGTGESGGNQIEELVYEGQGPAASQFIIKCV
                     TDNRNRTASTIRHLFSKAGGGLGSVAWNFAARGVVQIAAENLVSRQADWDNFELELID
                     QGAEDIVSENEGITIYTNPEDLPKLKAFLEQQAIATELAEVQQVAKEKLIISNPEDCS
                     RLEKFVESLDDCEDVSDYYTNAEWE"
     gene            14152..15228
                     /locus_tag="PKV49_02370"
     CDS             14152..15228
                     /locus_tag="PKV49_02370"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPL22488.1"
                     /translation="MVNLDNQTDCLALDSGQVGLAISLLPDQLSQVLQDFRHQPSWKL
                     NKPVNKIVLNGMGGSNLGARLIVSALADKLQVPLLIEPGYTVPGYIDNQTLYIISTYS
                     GTTEEPLSVIDQVQQRGAQLAALTADYPGNQLAEKARQHNWPAYIFNPQHNPSGQPRL
                     GLGYAIFGLLGLLANCHLVDFDLATAADLVVELQTTNQHWLPETPLTDNDAKQLAQQL
                     YGRQIWLIGPDCLTGNLHILRNQINETSKNLANYLTVPDLNHYAMEGLSFPTNIPETI
                     AILSFKITGLPDRLTKRLLLTDEVLEQNHLPVYNYQAAAANRLGQALEILAFGSWLSY
                     YLGLLNGVDPVKIPWVDLFKKKLA"
     gene            15245..16390
                     /locus_tag="PKV49_02375"
     CDS             15245..16390
                     /locus_tag="PKV49_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPL22489.1"
                     /translation="MTYQEGFWPLAGRCLFLLFWLLYPLMAMGQTTLHFQEIWPRPTN
                     GSPETLTLHNSSDQTIDLAGWQLADTAKQTKPISLSTYQLAAGQSWTIEAKTLGLSLN
                     NDQEELFLWQPDGQLADTVNYAKAPLDTAYQRGPDGWQWAQTLITAEPAVAKNQPDSP
                     ATTTQTVTGLVTALPYQLSSQYFYITYPDNSGGLKIYCYHKLWPQLTLGSSIEVIGQL
                     VSTTQEIKLNIHGQQDIKIISTTAPLIAKTITTQDISQLTLGQLVVFTGKLSAKNQST
                     LYLTDDFGELLVQLKTGSGLKNGDFTIGQIYQITGLILNNNGYRLVPRQPSDVIKLEL
                     ATSSAATTTLDFTADPKPTTSVLTWIVGLMAGFSLMTIAYILTKQQK"
BASE COUNT         4859 a         3956 c         3140 g         4609 t
ORIGIN      
        1 acccgcgacc tctgccgtga cagggcagcg ttctaaccaa ctgaactacg actccaatac
       61 cagtgtcgca ctggcaccga caagcttatc atatcatttt tggctcgtca ataagttatt
      121 ttttattatt tataatgctt taaaccgctg atctaaagcg cgcaccgaaa accaatatac
      181 ttatagtaat catttggagg aacatttaaa ataagtgtca aaacaccagc tcttgctcca
      241 ttattgcgcc aatagccacc gcgacgaaaa ccatagttaa tagtattggt gctagaacca
      301 atactaaaaa tttggccata cccctgatta ctattataat tattcattgg taataacttt
      361 tctggagcaa tatcgccgta attagtgagc acagtatatt caagccaccc agcttcactt
      421 accactccgc ttggctgatt tttgcccata atagtcgcat cggtccaatc ataaacatta
      481 ccggctagat cccaaataac ttcaccatta gataaaatgt gcgttctttt attaactccg
      541 tcaagctcac tggttccgtc catagccgca ctagaattgc tattaccgcg tgttatctga
      601 cctgagccga tagcgcctcc ggtccaatta ctggcttgta gctctagatt acgaacaatg
      661 cttaaccatt catcttcagt aattagatgg gcaccaattg attcacattc cagtttagca
      721 ttagtttgag taatattaac ccagggggtg ccgctaggtc gagattctgg ctcataactg
      781 gcactgtagg cggtattacc attgtcaatc cccttaattt tggcctcata tttcataaca
      841 caaaatgaat gggtattata taaagtattg ccgggaactc gcacccaatc ggttggacaa
      901 tcggcaatgg tttcttcatc ataaattaag ttaaagcttg aattattgat taatggagca
      961 taaagataat gtttgtcttt agccggatcc gctggtatat ttttcatata aacctcagtg
     1021 ccacaggtcg aggtaatacc taattcacta agacaaaggt tagtaattgg agttccaacg
     1081 ggattgggtg agttaggata aatgccatgg ctattataat atagctctag agctgtagaa
     1141 atttgtttaa catcggataa acgacgagca tctcgggctt tggcccgagc attatttaaa
     1201 gcaataaccg ataatgtgga caacaaagca atgatggcaa tgattactaa tagctcaata
     1261 agagtaaaag ccgctaaaga atactttgac tttttattta taactaaaaa cattaactac
     1321 ggataatagt aatttatatc tataatacca tactaatact tattaatggc cgagtcaaaa
     1381 ttgccaaata agctaaaatc tgttaaattc cagctgtaaa caaaatctat ggctttatct
     1441 atcggtatcg tcggcctacc aaatgtcggc aaatccactc tttttaatgc tttaaccaaa
     1501 aaacaggttg acgccgccaa ctatcctttt tgcaccattg aacccaatat tggcattgtt
     1561 aaagtacctg atgaacgggt tgatcaacta tcggccatgt ctcattcggc taaaaccatt
     1621 tataccacca tggaatttgt tgacattgcc ggtttagtta aaggcgctca tgagggtgaa
     1681 ggtttaggta accaattctt agctcatatc cgcgcctgtg atgccatttg ccaggtggtt
     1741 cgcaattttc aagacgacaa tattgttcat gtcgacggcc gcatcaatcc tgaatctgac
     1801 caggccatta tcaatctgga gttaatttac gccgacctag ccactgttaa taaacgattg
     1861 gacaaggtcg gtcgcgaagc taaaagcggc cataagacag ctatccaaca aacggctatt
     1921 ttaactaaat tgcgcgacta cctagataac ggccacagtt tgcgtcaatt tgattttact
     1981 gccgacgaac aggcgttgat taaagaatta aaccttttaa ccagcaagcc gattatctat
     2041 gttatcaata gcagccagcc ggaaactgaa caattaccag cctgggatgg cgcggttctg
     2101 ccgctcaacg ctaaattgga ggctgacatt gccgctctac cagaaaacga acaggctgaa
     2161 tatattcaag agctgggtct caatcaaagc ggcttgaaca aattaattgc tcaagcttat
     2221 caactgctca atctagtcac ttttttaacc accggcgccg atgaaaccag agcttggaca
     2281 gtaattaaaa atacaccggc tcctttggcg gccggcgtga ttcatactga ttttattaaa
     2341 ggttttgttc gggctgaagt tattaattgg caagatttac ttcgagccgg cagcgaggtt
     2401 aaagctaagg aattgggttt gctgcggcta gaaggtaaag attatttgat taaagacggt
     2461 gatgtctgta attttctaat taataattaa actttctcta agccaacaat cgctcgccaa
     2521 accacttgtc ctggccgacc ataaagcaaa ttgccgtcta aggtgtactg cttaggccaa
     2581 gcttgccggg ccaattggct atatttaacc ttacgccagc cagccatctt tggttggacc
     2641 aaatgctttt ggtgaccgtc aataaatacc ggccaaatca tcacgactcg cccacccgga
     2701 gcgattattt tttgccaatc aactaaagct tgactatata attgattcaa ttcctgagca
     2761 ataaccataa aattacgccg gccacgctgc ggtcctaaat atggttcagt cacaatccgt
     2821 ttaattcttt gattagctag ctggactgag gcctgacgag cgtcggctaa actaatttct
     2881 gggtcaatct caattggcca acggttagct aaccactgcc aattaatttg actattggca
     2941 acggctaccc ccgaattatc cagaccaata attcgcccgg ctttcattaa agctgcctcc
     3001 tgcaaaatgg tgccagagcc gcaaaatgga tccaaaacac tatcacccgg ttcaaagcca
     3061 gctaaattaa gcataatctg ggcaagcttg ggcggtaaca tgccggactg atcatcacga
     3121 gccgggcggc cataatcacg agccgataac gacttaaaag gctggacagc caccgtcact
     3181 ccccattgcc aacaattatc ttgtcgccat aaaactactt ctccgccgct agttagtaaa
     3241 tgattctgct caactactac actggacaaa gccgctgcgc gactaattac ccagcgcgcc
     3301 gaacgaccag tatcttggat aaaacgcttg atttgtatac ctaaatcacg gctaagcggc
     3361 tggcgatcat aattagataa accgaaacaa atttttttgt cagccggggc gaccgccaat
     3421 aattgcttag ctattacttg agccgccgct tcattaagtt gattaaattg accaataact
     3481 cgaccaaact taatggttcc accaagatat tctaataaat ccgccgataa ttcggccact
     3541 tgactggcta gcgccaaatt atcgttaacc acctgccagc gacccgacaa tttgactgcc
     3601 aattcagtta aggacaaggc tggatggctg cccaagataa aaaaataatt catataccgc
     3661 tacgctaaca taaaaccgcc agtcttgcaa ttttttaact tctgtgttag ggttaatgac
     3721 acaaaaagac aatctttgcc ttttccatgt ttgaaccaac cattggttta aatggtgcta
     3781 ttaataatta gtggcaccaa aggcaggccg aaagcagtta cccctctgcc tgcagactta
     3841 tggttgaggg ttttattttt attattaatt attctttacc cattacttat ggctaaaaac
     3901 aaaagctctc ggttaccgcg ctatgaattg ctgtatatcg tgtctaacaa atttacagcc
     3961 gaggaaaccg agccgattgc tcaaaaaatt gaaaatctaa ttaccgctaa cgacggcaca
     4021 atcgtttatc gcgaaaactg gggtaaacgc cgaatgtctt atccgattaa aggttttctc
     4081 ttcggttatt atcgtcttct ggagtgcgat ttagcagccg ataaaattgc tgaggttgag
     4141 cgctttttgc gcttgtccag cgatatcttg cgccaccaaa ttgtcaaacg ccaaccacga
     4201 actgttgaag aaattctagc cgctaaagaa gaaatcgcca agcgtgaagc caagctgcaa
     4261 tcagataaaa ctgaagctga ggccaaaaaa aataatccgg aaaaagaagc cgctactccg
     4321 gctagcgaca gtgaagaagt ggccaaaaaa catgatcaag atattgacgc caaaattgat
     4381 accttgatca atgaagaacc aatcgtcaaa taaattttaa tcttctaaat tacgcctatg
     4441 gatctaaaca aagccatgct catcggtaat ttgactcgtg atccggaaat ccgcaacacc
     4501 accaccggac agccagttgc ctcatttaca gtggttacta attatgtttg gaccgaccaa
     4561 actggccagc gtcaagaaag ggccgaattc cataatattg tcgcttggcg ccgcttagcc
     4621 gatatttgcc gccaatactt gtacaaagga tcaaaagttt atgtggaggg acgactgcaa
     4681 acccgcgatt ggctagccca agacaatacc aagcgctatc ggaccgaatt agtagccgac
     4741 aatctgatta tgctagatcg agccggttca gctagtgcac cattctccgg accggccgct
     4801 gaacaaaccg acaacaacga accatcgctg gagtcattgc ctgataatcc ggccgaagaa
     4861 gaaatcaaaa ttgaaaatat ccctttctaa attatttaat tgcttactat gaacagtcgc
     4921 aatagcaaca agccttgtta tttttgccaa aatggcatca aagaaattga ttataaagac
     4981 accttcctac tgcgccgcta cgccaacgct ttaggcaaaa tcttacctaa acaccgcacc
     5041 ggcacctgcg ctaagcacca gcgccaatta actttagcta tcaagcgggc ccgcatcatg
     5101 gctatgttga tgtttgtccg ccgctaagac taacctatgt tatcattaaa tgaaatcaag
     5161 ctcggcaagg tcatcaccat taatggcgag ccttattcgg tcattcgcgc tgaccatcac
     5221 aagatgggcc gcggcggcgc cgttttgaag actaaactgc gcaatctgat tagcggttcg
     5281 gttttggaaa gaacctggca aggtaatgat cgggctgatg aggctgacgt tgaaaaacgc
     5341 aaagctacct ttatgtatac tgacaaacag tttgccaatt ttatggacag cgccgattat
     5401 gaacaattcg ctatcaattt agaagaattg ggcggcaaag aaaaattcct taaagacggc
     5461 ttggaggtta ccgttctcta ttttgagggt cgacccgtgg ctgttgaatt gccagtcaaa
     5521 gctgagtaca aagttatcac cgccccgccc ggcgttaaag gtaatactgt cggtaatgtt
     5581 atgaaacaaa ttgaaattga aactggcgct cagctcaatg ctccactatt tattaatgaa
     5641 ggcgatatta ttcgcgtcaa cactgatact gaagaatacg tagagagagt taattaaatt
     5701 ctagttctct cttacataaa aagacccggt ctttcggccg ggtcttttta ttgataatct
     5761 atttatttta ttcctgttta gctgccaaat caatcaagac ctgttttcta atcttcttca
     5821 ttaattcttt atcctgccgc aaaaaagtct tagcgctctc ccggcctaca cctaacttga
     5881 tttcaccaaa gctataagaa ttgccactct tggtaattgt tcccatttct acgccagtat
     5941 ctaggacatc gccggccaaa gaaatacctt cattatacat gatatcaaat tcgcagctct
     6001 taaatggcgc cgccacctta ttcttaacca ccttacattt aacacgattg cccagcattt
     6061 tttcaccctg tttaatctga gcaatcttgc gaatatcaat tcgcaccgag gcataaaact
     6121 tgagagcatt accgccagtg gttgtttccg ggttgccaaa cattacccca atcttcatgc
     6181 gtaattgatt aataaaaata acaatcgttt tagatttggc aataacgccg gtcagtttgc
     6241 gtagcgcctg gctcatcaat cgcgcctgcg aacccatttg taaatcgccc atatcgcctt
     6301 caatctcttt ctttggcact agggcagcca cgctgtcaat taccaccaca tctaccgcat
     6361 tagaacggac cagtgtttcc aaaatctcca aagcctgttc gccggtgtcc ggctgactga
     6421 ttaacatttc atcaatatta acgccaatct ttttagcata ttccggatcc aaagcgtgct
     6481 cggcatcaat aaaagcggcg attccgccct gtttttgaac ttcagcaata atatgttgag
     6541 ctagcgtggt tttaccacta gactctggcc cataaatctc aatcactcga cctctgggca
     6601 cgccgccgac accaatagcg agatccagcg ataaacaacc ggttgttacg gcgtcaacat
     6661 tggtgttttt aacatcgccg aatttcataa ttgaaccctc gccataaagt tggcgaattt
     6721 gactcatggc cgcttcagcc gccttttgct tttccgataa attaccgctg tctacgctgg
     6781 ctagttcttt gtctttcttg ttttttttgt cgttagccat atgaaaaaaa ttaaaaataa
     6841 atgggggtta attaatttat tatagcacaa aaatctttat tgcagcagcc ggcgatacag
     6901 ccgtaaatat tctttggctg gctcattcca cgaccaatct tgattcatgc ccactagttg
     6961 tcgctgacgc caaattgctg gtcgttgacc atattcatcc aaagccttat caattgttga
     7021 ataaaactca gtcttggtta gtttattaaa taaaaaaccg ttatttttat taaccgtatc
     7081 ttttaacccg ccggttgatc gcacaattgg cactgcccca tagcgcatgg ccatcatctg
     7141 ggttagtccg cacggctcaa aacgcgaggg caccaagaat atgtcagccg acgcataaat
     7201 ctgatgagct aaccgatcgt taaatttaat ttgagcgcta atccgatcag gatatttagc
     7261 cgccaaagcg gccaactgct tctcataacg cggctcgcca gtgcctaaaa acacccactg
     7321 acaattacgc ttaatcattt gttgatcaaa taattctaag cctttttgcc aaaccagccg
     7381 cgaaatcaat ccagctagcg gaatattagc atcttgtttt aagcccagct ctttttgcag
     7441 cgccaattta ttaatttttt tccgttccaa agtctgagcg ctaaattttt taactatatt
     7501 tttatcctga gccggattat attctacaac atctataccg ttaataatgc ccgacaaatt
     7561 acgccggcgt tgccgtaaat agcgccccag tccggcgtct aacggccggc ttaaaatctc
     7621 ttgagcgtag cttggactaa ccgtagtaac ccaatcggca gttttaatac cttcagccat
     7681 aaaattgagg tcgccctgcc aattttttag ccctgcccat ctgacaatat tgtgcccagc
     7741 ccagccctga ttagctaaat tatgaatagt atagactact cggctaacct cataaaaatc
     7801 acttggccat tgatgcagca aatgttgtac taacgccgtg tgccaatcat ggcaatggat
     7861 aatctccggt tgaaatttta aagcctcagc ggccgccaaa gcagccttag taaaaaaact
     7921 aaacttacgc acatcatcaa ttttacgatt aggtttgtcg tctggatcat tcggttgcag
     7981 ataaatcgtc gctgtgttta aaaacagatg atcaagcaaa taaaccggca cctggctgcc
     8041 aggtagtcta gtctgataaa ttctaatttt ctcagctccg gtcgaccagt taacaatagc
     8101 cgtagtcttt aacggtttta tttgataagc gcgccgatca atcaaaccat agagcggcaa
     8161 acagatccgc acatcaacac caagcttagc taaagccttg ggtaatgccc cggccacatc
     8221 acccagaccg cccgtcttgg caaatggcgc cacttcagct gataaaaacc aaaccttaat
     8281 cttttttttc ataaattatt aaccaattat ttcattaata cgttgtaaat cttgactatc
     8341 ggcttgccaa taatcatagg cgctcttaag gccgcatgaa acttcttcaa aacgatgaaa
     8401 atctgaccca gccgtaataa ttagattata gcgtttggct aagtcttgag catacataat
     8461 cgcgcccaaa ctatggtgcg gtgacaaaac ttcaatacca tcaattccta aattttttaa
     8521 tctatgaact aggctgcgtt taatagaacg ccctttggct ggatgacaaa aaaccagttg
     8581 tccacctaac tctcggcgta atttaataac tcgattaata tcaatacagc tcaggcgtaa
     8641 gcggccaatt tttttattac caaataataa accgatggcc tcctcttcgc gaaatcggct
     8701 atcatcaagt cgctcaacaa taatttcccg attaccagga actaacagta aagcatctaa
     8761 taaaccattg gtaggaataa aattggtaaa tttttctacc actcgatgac tatcttttac
     8821 taataattta gctttaacta atttctgcaa atgctgcttt aggcgcactt tccaatggcg
     8881 ctgactagtt aataataatt tagcaacttg ctgatcgcca attggcaaat tataccacag
     8941 taaattaatc tgctgattat ttaaccgcgt atataactca ataccgggaa tcggtttaat
     9001 ttttaatttc tggcaagctt gataaaactc cggccagcca gccagcgtat tgtgatcggt
     9061 tagtgaagcc actttaactc cctgttgagc taaaaattta gccaccgctg tcggtgttaa
     9121 ataaccatcg gaataaatag aatgaatttg caaatctatt agcatatact caaattaaat
     9181 taaattccac ctcggttaat aggccgctgg cacgctgcgg ttctggcccc gatttagtga
     9241 caaaataaac agctgttcgg aaagccgcaa aattataata agtttccaaa cgctctgacc
     9301 agtcagccgc cattacttcg ccaacgatcg tttgataggc ctctaagaaa aacttctgcg
     9361 cctgctgcca atatgcaata tctggcaaat gacgctgacc cataaaagct agctgttgaa
     9421 caaatgcacc taaatcgcgg gctggatcac ccagcgaagc atcggcccaa tcaatgatgg
     9481 ccgctctttt ttgattaacg atgatatttt ctgggtgtaa atcaccatga atcatggtta
     9541 gattatttcg gctcggccaa tgttcggctt cgcgcctctc caaaatatca taaagctgga
     9601 cgaaacgact atattcttgc ggacaatttt gtcggatttt atttaaggcc cgctggcgtc
     9661 caggcactac ctgaccaatc agctgttgcc gactatctgg ccaatcacgg agtcctttgg
     9721 ttaatttatg cagccgtgct agccagccag cgattgattg caaataatca tttaattgat
     9781 ctaactgtcc ggctttaaaa tattgatcaa gattacgccc cggtaatccg gtataaaaca
     9841 gaccgcgaca atctggatca tagaataatg gccgattggt taaaaactgc ttattggcta
     9901 tttgtttatc ccataataaa cgcaaattat cccaagtctg gcgacgtgat tcgctgctgt
     9961 gcgccgaaca atagatagag agtttaatat cagctccgcc ctgcttagct attaaatcat
    10021 aggctatcac caagctgtaa tactcatggt gaaaattact ttttatcacc ttaaccttct
    10081 ccactactat ttcggttata gccggatcga tttcggcttt acgctgatta aataattgct
    10141 taaccttgat tgggtccaaa atatcttgaa tactggccat ataattaacg tttccactgc
    10201 tgccaatgtt tctgccagat tctggtctgc agtgaacaaa atattttatc agcttgctgt
    10261 ttccatggac gactggctgg gttatcaata gaacgaacac ttttaactaa ttgctcagcg
    10321 ccgcgaatga tttcctccgg accccaagcc gccgagccaa acaatgcaaa atcgcgaccg
    10381 ctcgcccacc accaagcaca actagatagg ccttgatgca agtgacgcgc cacccatttc
    10441 tgattactat caccagcaaa acgctgcgcg gcctgttgag ctaagtccgc taacgaccac
    10501 aacatttttt gtagatggtt agcgcggcta tcccaaacag caaacggttg cttggcggct
    10561 atttcatccg gccagctctc ccaagaagcc gccactaaac taatggctgt caaattatct
    10621 tgactagcta ttgcttgact gactgtctcg gtttttattt ttggattacg ccaacacctt
    10681 tctagcttac cgctataatc acgatgccga aggccatata gctcgccatc ggtgacggtg
    10741 aataaatttt gtggacaatg atcgattcct tgcgttaata tttctggcgg atatttctta
    10801 gaccagtcgc gttgtctaac cgctaaaact aacccggaaa cctgatcacg ataaaacatt
    10861 tgcttgggag tatctaagcg accatcaatg ctaatttcat caacaatagc gtaatgatat
    10921 ccgcgttgag cgagccattg aattaactct ggcgaatagg ccaattcagg agcaaaaaaa
    10981 ccggctggcc gagcaatttt aaatagacgc gctaatattt tttcttgagc cgctacttga
    11041 ctagccaatt cttcaccact gatctttgga agtaaagcat gccaataggc cgagcccgtt
    11101 aactcgacct ggccgctagc aactaacctt tgccaatcgg cgatcagttc gtgctgattt
    11161 aacaacaata gctgttctaa taagcaaccg gcaaaattga ccgtcacgcg gctattgggt
    11221 gattttaata aagccttagt cactcgacgg tagcttaaac ggacagcttc cgcaacaatt
    11281 tctggacggc ttaatggtgg ttgataaaaa tgtaggacat taacccaacg cataaattta
    11341 ttttaaagct cgccgataaa gattgatata ttttttggcc ggaattttcc aagaactggc
    11401 tgttttcatc cctctcttaa gcaaaccggc ccaactggac tgatggtgat aattctctag
    11461 cgctcggata atagctgtgt atagagcaat agctgtgaaa ttattaaaaa caaaaccggt
    11521 accgcgattg gttcgcgggt taaaattatt gaccgtatca ttcaagccac cgacattgcg
    11581 cacaatcggc acacagccat agcgcatagc aatcagctga ttaataccgc aaggttcgtg
    11641 atgcgatggc agcaacaaac aatcgctggc agcgtaaatt agactttcta attttagatt
    11701 ttttttgtat gacaagaccg ccaatctccg tggatatttt ttgcgccaag cttttaattg
    11761 attaatatac tggctgtcac catcacccaa aatgacaatc tgcacatcgc gtttgagcaa
    11821 ccagcccata atttccataa ttaagctaaa acctttctga aaagttatcc gcgaggtggc
    11881 acacagcaac ggcatatcgc gatcaacttg caagcctaat ttttcctgta aataggcttt
    11941 attattagcc ttgccgacaa tggcggaccg ccaatcatat ttagtaaata aactatcgtc
    12001 attgctcgga ttataatcaa aatgatcaat accattaata ataccaaata accgatcaat
    12061 gcgattacgc aaaattcggt gcaagtcttg accgaagtgg cgagtcataa tctcctcccg
    12121 atattgttcg ctaaccgtgt tgataatatt agcctgcagg atggcgcgct tggcaaaatt
    12181 aacatattct agatctggat tagttaaatc cggcaatttc ttagtgccaa aatctttctt
    12241 atcagccggt gtttgccacc aattatgacc gaattgaaaa attaagttgt ggatagtaaa
    12301 aaccgtccgt gctccgcgca aaaccgctga tttcttaaat tggctattta ataattgcgg
    12361 aatcaatccc gtttgccaat catggcaatg aataatatcg ggtttaaatt ccaataattc
    12421 caatagccgc aaaacggcaa catcaaacaa ataaaaacga gcattatcat tagctaaacc
    12481 ataaatatct tgcttcttgc caaaatattc tttattctca acaaaataaa ccggcaaccc
    12541 ttccatcaga taaccctgcc aataattaac agcgattgtt ttaccgccaa ccattaattg
    12601 aatatcctga aaaatctctt ttagcttata tttttgttta ttgattactc gattataaag
    12661 cggtgtaata actattacct gatgacctaa acgattgact gctttgggta aactgcgcgc
    12721 tacatcagct aagccgccgg ttttgctaaa cggcgccact tcagaggcca cgctggcaat
    12781 tttcaaaatt cggctcatat atattaatgc ttatcactgc tagccaatag agtcgcttgg
    12841 ctttgggcgg cgcaaagagc ggccgctaga ccatcggcgg catcgtcggg ccgcggaatt
    12901 tctttaaggc gcaaaattag ctgaaccatt ttctggacct gccgcttgtc ggcttgacca
    12961 tagccagcca ctgcctgttt gatctgtaac ggtgtaaatt ccaaacaagg aatcgcctgc
    13021 tgaccaatgg ctaacatcac cacaccgcgc gcttggccaa cctgaaaagc ggtggtaaca
    13081 ttgcgacaaa aaaataattg ttcaatacca acaatgtctg gctgatactt ggccagcaaa
    13141 tctttaactt gctgataaag actagccagc cgctccggca acggtacttt agctgcagtt
    13201 ttaagtgagc catactccag acaatctgta ttgggaccgt ctaaccgaat aacgccaaaa
    13261 ccggtatcag ctaggcctgg atcaaaacca ataataattt ttactgcctt agccattatt
    13321 cccactcagc gttagtataa tagtcgctaa catcttcaca atcgtctaac gactcaacaa
    13381 atttttccaa ccgtgaacaa tcttcaggat tactgattat taatttttct ttggccacct
    13441 gctgtacctc ggctaactcg gtagcgatag cttgttgttc taaaaaagct ttgagcttgg
    13501 gtaaatcttc gggattggta tagatagtta tcccctcatt ttcgctaaca atatcttcgg
    13561 ctccttgatc aatcaactcc aattcaaaat tatcccaatc agcttgccga ctaactaaat
    13621 tctcagccgc aatctgaact acgccgcgag cggcaaaatt ccaggccact gaccccagac
    13681 cgccgcccgc tttagaaaac aaatgacgga tagttgaggc ggtccgatta cggttatcag
    13741 taacacattt aataataaat tgactggccg ccggaccttg accttcataa accaattctt
    13801 caatctgatt accaccactc tcgccagtac cacgcttaat ggctcgttca atattatctt
    13861 tcggcatatt ggcttgtcgg gctttatcaa tcgccattct caagctgaaa ttggtagctg
    13921 gatcaccacc tttatcacga gctgcgatgc taatcaggtt gcctatttta gtaaaaacag
    13981 caccgcgctt agcgtcaacc acggcttttt ggcgtttagt agtcgcccac ttagagtgtc
    14041 cggacatagg gattagctga agagtaaaat cttattgtca aattatcaca ttttttgata
    14101 aagagcaata ttattgctat aataatcaaa cttatttcct taatttcacc tatggttaat
    14161 ttagataatc aaactgactg tttagctcta gatagcggcc aagttggcct agctatctct
    14221 ctgctacccg accaactatc acaagtttta caagacttcc gtcatcaacc gagctggaaa
    14281 ttaaataagc cggttaataa aattgtgctc aacggtatgg gcggttccaa cctaggcgct
    14341 cggctcatcg tctcagcctt ggctgacaag ctgcaagtcc ccttattaat tgagcccggc
    14401 tatactgtcc ccggctatat tgacaatcaa actctttata ttatttcgac ttattctggc
    14461 accaccgaag aaccgctgag cgttattgat caagttcaac agcgcggcgc ccaattagca
    14521 gcgcttactg ccgactatcc gggaaatcag ctggccgaaa aagcgagaca acataattgg
    14581 ccggcctata tttttaatcc ccaacacaac ccctccggcc agccccgtct cgggcttggt
    14641 tatgctattt ttggtttact gggattactg gctaattgcc atctggttga ttttgaccta
    14701 gcgactgctg ccgatttagt ggttgaatta cagaccacca atcagcactg gctacccgaa
    14761 acaccattaa ctgacaatga tgccaaacaa ctggctcaac aattatatgg ccgccaaatt
    14821 tggttaattg gcccggattg cctaactggc aatttgcata ttttacgcaa ccaaattaat
    14881 gaaaccagca agaatctagc taattattta actgtccccg acctgaatca ttatgcgatg
    14941 gaggggctgt cttttccaac caatatcccc gaaacaattg ccattctgtc tttcaaaata
    15001 accggtttgc ctgatcggct aactaaacga ctactgttga ctgatgaggt tttggaacaa
    15061 aaccatctgc cagtttataa ttaccaagcc gcggccgcta accgtttggg tcaggctctg
    15121 gaaatattag ctttcggcag ttggcttagt tattatttag gtctgctcaa cggtgttgat
    15181 ccggttaaaa ttccatgggt cgacttattt aaaaagaagc ttgcttaatc tttaacataa
    15241 acctatgact taccaggagg ggttctggcc attggccgga aggtgtttgt tcttattatt
    15301 ctggctgctc tacccgctaa tggctatggg gcaaactact ctgcacttcc aagaaatctg
    15361 gccgcggcca accaatggtt cgccggaaac actgacgctg cataattcga gcgaccagac
    15421 aatcgatctg gctgggtggc agctagctga cactgccaaa caaaccaagc cgatcagtct
    15481 gtcaacttac cagctagcgg ctgggcaatc gtggacaatt gaagctaaaa cattaggcct
    15541 gagcttgaac aatgatcaag aagaattatt tttgtggcaa ccggacggcc aattagccga
    15601 tactgtcaat tatgccaaag ctccgctaga cacggcttat caacgcggtc ctgacggctg
    15661 gcagtgggct caaaccttaa tcacggctga accggcggta gctaaaaatc agccggattc
    15721 gccagccacc accactcaaa cagtaaccgg actagtcact gccttgccct atcaattatc
    15781 gagccaatat ttttatatta cctacccgga caattccggc ggtctaaaaa tctattgtta
    15841 ccataagtta tggccgcagc taactcttgg cagctccatt gaagtaattg gccaattagt
    15901 ctccaccacc caagaaataa aattaaacat ccacggccaa caagacatta aaatcatatc
    15961 gaccaccgct ccgctaattg ccaaaaccat cactacccaa gatattagcc aattaaccct
    16021 cggccaatta gtcgttttta ccggcaaatt atcggccaaa aatcaatcta ctctttattt
    16081 aactgatgat tttggcgaat tattagtcca attaaaaact ggctcgggac ttaaaaacgg
    16141 tgattttact atcggccaaa tttatcaaat cactggccta attctaaata acaatggcta
    16201 tcgtttggta ccacgccaac ccagcgatgt cattaagctg gaattggcga ccagctcagc
    16261 cgccacgaca actctggatt ttacagccga cccaaaaccg accacctcgg ttttaacttg
    16321 gatagtcggc ctcatggctg gctttagtct aatgactatc gcctacatat taactaaaca
    16381 acaaaaataa ttgactatta acccgaattc agctaaaaac tttataataa cttacctaat
    16441 taaattatat aatcaaccag cttaacatca ataaatacaa taaaaaaaca aacctcgcgg
    16501 tttatttttg tggacgatac tggatttgaa ccagtgacct ctacgatgtc aacgtagcgc
    16561 tcta
//
LOCUS       DAOPAI010000014        15321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_3925_length_15321_cov_4.319141, whole genome shotgun sequence.
ACCESSION   DAOPAI010000014 DAOPAI010000000
VERSION     DAOPAI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3925_length_15321_cov_4.319141"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKV49_02380"
     CDS             complement(<1..451)
                     /locus_tag="PKV49_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPL22490.1"
                     /translation="MIKFKQASKFYPPQTKALDNLSLNIGPGEFVSIVGQSGSGKTTM
                     VKCLIGEERLDSGQIIVGDWDITRLSSSEVPFLRRQIGVIFQDFKLLPKKTLAENVAF
                     ALQVCGKSSKKINAIIPPLMKIVGLSEQMDRYPHEVSGGEQQRTAIAR"
     gene            complement(465..1814)
                     /locus_tag="PKV49_02385"
     CDS             complement(465..1814)
                     /locus_tag="PKV49_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HPL22491.1"
                     /translation="MDSSRSNFYWLKIGRLLFIIAGLILILAQPILTSANAAVSATSS
                     PSGKASWYNFKPGLFAASTIYPLGQKVRVYHLASGKTVDVVINDYGPDPQAHPDRVID
                     LQKEAFAQLATLGTGVITVRLEPLSPSSTSSASNNKPESPKPTRDLAKERLALQQFTK
                     KYGRLPKNSADWQIINNLAYAASSTKTVSDFKSLEISNQDANSVVVLDADNNQVLWQK
                     NAQKIVPIASLTKMVAIKTFLDTKPNLNKVVSYRRQDEQEITKYCALSEAALLKLKDG
                     DQLKIRDLVYSALIGSANNAVESLVRVSGLSRAEFIQKMNANAKKWGAINTKFVEPTG
                     LSTDNVSTAKDYALILKAVSQDANIKKISTTAIYKFSTTNTKQPHTIKNTNKLLQNGQ
                     FPLQSSKTGYLVEAGHCLAMAAQNQNTKQKFIVVTLGSSSWSKSYATASQILTTALN"
     gene            1900..1971
                     /locus_tag="PKV49_02390"
     tRNA            1900..1971
                     /locus_tag="PKV49_02390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1933..1935,aa:Gly,seq:ccc)
     gene            1984..2056
                     /locus_tag="PKV49_02395"
     tRNA            1984..2056
                     /locus_tag="PKV49_02395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2017..2019,aa:Thr,seq:cgt)
     gene            complement(2084..2518)
                     /locus_tag="PKV49_02400"
     CDS             complement(2084..2518)
                     /locus_tag="PKV49_02400"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPL22492.1"
                     /translation="MNKLPSKNRHKRYQSILITIAGLLALIFILTGLVKNIRQVFGVK
                     SEITQLEAEIKRLDKKNQEVADLVDYLDSKEFLEEEARLKFDLRQPNEQIIVIKQSDS
                     STDAADHSANSVFDLPAPQKEKVAISRNPSLWFKYFFDHKVQ"
     gene            complement(2521..2591)
                     /locus_tag="PKV49_02405"
     tRNA            complement(2521..2591)
                     /locus_tag="PKV49_02405"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2557..2559),aa:Pro,seq:ggg)
     gene            complement(2618..3112)
                     /gene="ndk"
                     /locus_tag="PKV49_02410"
     CDS             complement(2618..3112)
                     /gene="ndk"
                     /locus_tag="PKV49_02410"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPL22493.1"
                     /translation="MAKKRQERTLVIIKPDALNRSLAGEIIHRFERKGLKIVGLKMMQ
                     LDNAILAEHYSHLIDKPFFPRIAKFMSDWPVIVMALEGMEAVNSVRLLTGETLGRKAD
                     AGTIRGDFSMSIQANVVHASDSVDNAHKELARFFQPEELFDWDRLDLQAIYAEGEGDE
                     EEEE"
     gene            complement(3201..4052)
                     /locus_tag="PKV49_02415"
     CDS             complement(3201..4052)
                     /locus_tag="PKV49_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPL22494.1"
                     /translation="MKKLFIWLALGGLISLIIIIIATTKSSVSSWLRISYLDIGQGDA
                     ALITTTSGQNILIDCGPNQQILSALSRKLRWWDRHLDIIIISHDHADHWGGLPYLINK
                     YPIQQIILAQPPQLSTELANVLNDAQQKGIIINDVRAPAVIKLSDNSQLTILWPTNEQ
                     LIRYQDNINNQSLVILWQYGHNKFLWTGDLEADAESELLAQQPIGQPIDILKIAHHGS
                     LTATSLAWLNYWQPKIAVISLASTNKFGLPSQLIIDRLRRLGIRLWRTDQLSDFNILS
                     NGQLIWQ"
     gene            complement(4054..4719)
                     /locus_tag="PKV49_02420"
     CDS             complement(4054..4719)
                     /locus_tag="PKV49_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPL22495.1"
                     /translation="MLGGEQKARHGLFWLDLLWPKHCYHCQQPGSWLCHDGQQQLTAN
                     WHWRSLPIDHKSSLTAIIYCADYHQPLLNKLLKLTKYQRLTIAGRILAINLAEFIQQT
                     IAEQLWFKQSLITAVPLSRQRLNWRGFNQAQLLVETIAKVNNCPTASYWRRQHRPPQA
                     HLNKNARLTNLDQAFSWLKYSAGQNFIIIDDVVTTGTTLTEMANCLKQSGANEIRGLA
                     LAH"
     gene            complement(4735..5361)
                     /locus_tag="PKV49_02425"
     CDS             complement(4735..5361)
                     /locus_tag="PKV49_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22496.1"
                     /translation="MRLSPKTINWLMIGGVIIILIVLLYILLSGKLNPSYNPPATIVS
                     PTTTTPEITQPEPQTFSTEQLANNQIILDNRGRLEIELEKKAGAFVERWASLSSQDNF
                     SNLEILKNSMTQSMIAWTDDYIKNQSATKKTDTYIGTRAQAIVTQGTLDSVDSTSLTV
                     DVKIRGQETIATASTQPKNFYQNYQLSLVKQNDVWLANKLTWQDRQYQ"
     gene            complement(5382..7268)
                     /locus_tag="PKV49_02430"
     CDS             complement(5382..7268)
                     /locus_tag="PKV49_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL22497.1"
                     /translation="MFNIQPNKKTTSPDKANDISFLNKQTSAALAEQARLAEELIEQE
                     RAYRYGVVNIRDLIAPSSLEVKPKYLLLNGQYVSTIFIVAYPRYISVGWFAPILNLNE
                     SFDISMFFYPINSAIVLKQLKNKAGVLEAQISADIDRGNPRDPLRETALRDVEALRDS
                     LTQGTEKFFQFALYVTIYAKTEEELNQLANNIEDLFGSRLIYSKKVFYQAEQGFNSTL
                     PLCDDGLNIAFNMNSSPVASSFPFISSELTSDNGILYGINRHNNSLILFDRFSLPNAN
                     NVVFATSGAGKSYAIKLEVLRTMMMGTDVIVIDPEYEYKELAEAVGGTYINISLASEA
                     KINPFDLPRPVGQESKTEDIIRSGVITVKGLVRIMLGQLSAEEDSIIDMALLETYAKK
                     DITADSDLSQIEPPTMRDFQYILEGMEGGENIALRLKKYTEGTFSGLFTSPTNVQMDN
                     QLVAFSVRDLEDELRPLAIYTIVNYIWNVVRSQLKKRILVIDEAWWLMQNEDSAKFIY
                     ALVKRCRKYYLGVTTITQDVNDFLLSPYGKAIVTNSAMQILLKQSPAAIDLVQKTFIL
                     TEGEKYLLLESGVGEGIFFAGNKHAAIKIVASYIEDQLITSDPEQLLKMEKQNQAEAQ
                     AEAE"
     gene            complement(7277..7954)
                     /locus_tag="PKV49_02435"
     CDS             complement(7277..7954)
                     /locus_tag="PKV49_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22498.1"
                     /translation="MASQQKNNPISTQRYLKIADIKKDTVIMKDGTLRAILMVSSINF
                     ALKSEDEQEAIISGYVSFLNNLDFPIQVVVQSRELNIANYLESMRKKANEQTNELLKN
                     QTLQYIDYVSQLVSLGKIMNKHFYIVVPYNPISDKRKGFWQSLIEAFRPIDVINLKEE
                     RFIRLKKQLSERIVSVINGLSSMGLNVVQLDTQGLIEMFYNVYNQETAANQPLDNVEK
                     ININNNF"
     gene            complement(7957..8415)
                     /locus_tag="PKV49_02440"
     CDS             complement(7957..8415)
                     /locus_tag="PKV49_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HPL22499.1"
                     /translation="MQQFTVPQFIDAEDKIIGPITTRQFIILLVMSGVIFLEYKLFDF
                     SLFLVATLLTAGVFFTFAFGKINGRPFHYFLLNLIQSLFMPRLRLWNKNFGKNLAEAQ
                     FDSKNIIKAQPPAVKNRFTSSRLAELSLIVDTRGAYGGETELIDELITKP"
     gene            complement(8418..9719)
                     /locus_tag="PKV49_02445"
     CDS             complement(8418..9719)
                     /locus_tag="PKV49_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22500.1"
                     /translation="MFDYLQKYQQVPADVRAKLDTPLIRATIKQLEETYRLSLASLLI
                     KIMVKDISPSRLSYYLSENYGLSGDQAQKLTAELQAKILVTVQSYLAKPLNAPIDINQ
                     LAKGGTLKALTATSQPPLNQKADPSSTVNIDLESVLNKLVSQQKIFKQPELINRWRQL
                     AKTFLIGVRNQRAVDDFLSRPLTSGGLGVSANQKQQLNQQLIEAKELLHRAAEQQVAV
                     APPVKPVDQLINQTAVRDFESDLLASLKKIDNRPRPDLKTESELPAPPANNSKLLEAA
                     RPAEKNSTDDQQVAAVSTDSAPTIVVPIDQRATDNKTGKIRMDDVRFTPKTLTPIDEL
                     ANLNLKRFRYLGRSPEERTQKIKEKLDMLAEHGYSKRLQGLSAWRQSPLNRLYLQIGQ
                     RSIQNCQPAEQLLSKEDNPDNDNLTFNEFIAIMNLNKQIRF"
     gene            complement(9723..10742)
                     /locus_tag="PKV49_02450"
     CDS             complement(9723..10742)
                     /locus_tag="PKV49_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22501.1"
                     /translation="MSTKQYLYSCLDKFYELPDEVILEFNHPDRLKIIDELAAEYGLE
                     LSFLVILIAINELSLDNLTEYLMKRYDLPERIAATITNKLNSLYFEPIIKRLSFFNQD
                     PHKISPTLDEEKSIIINLFKENLLVELKNYEQIIAAINQRIFAWLDRDLSARKQIETA
                     LLQNQEKVTDQSLVINNEKFPGTVTNWLRCFAAEKGAENFNSLSISEFLGLSKNTTNL
                     SDSDKKILRALFNTYRNIKFFPDSMPNDDGTDWAIIPLPEKNVSFDETIDLTSDSTTS
                     HTTAQSIDPEMQPLVAPTKSAAAPKDEIVINKINQLQLMLKRYNPDSLEAQAITEEII
                     KLKKN"
     gene            complement(10748..14377)
                     /locus_tag="PKV49_02455"
     CDS             complement(10748..14377)
                     /locus_tag="PKV49_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22502.1"
                     /translation="MWRKIKTFFSNSWGQLSPKQKYFVAGLILLLIFTPIFVFALDII
                     TTAILKILTGIISILVSFFGKLLAVVLHVLVYVAQYNNFINEQVVVEGWKVVRDVCNM
                     FFVLGLLIIAFGTVLRLQNYQAKNLLRSFILGAIFVNFSKTICGLILDFAQIIMLTFV
                     NGFKDVGAGNLTEMLGLTALFDVVVNNSVQATDAPLEGLGVFISYLLALIYIIVALVV
                     ILTMAIMLIQRAVMLWIYIVLSPFPYLLSALPKAGKLGQAGSRWWSDFTGLVITGPML
                     AFFIWLSFFTASGSGVSNSVNKDNEIKLTGGSGQAIDPNQGSDLTDDINAGVTKSGTA
                     QAIIKFIVSIGLLWGGMMLTKETSSLVGGVVGAGMSNLKKMGNKVKKTAGGMATTTAK
                     WAGRNALKGAGGLTYAMSKNSNSKVGQMFQKLGTAGKTWGDELTKERKEEKTKKRLKT
                     LKKLGLGVGDKTMAAFKEVANDDVVKTIGKTVKGGLTMAGGTAAGILTGNPFLGALMI
                     GGSTAAGLAQSPLNKVIGGGIKRMIGGTTVPAYEDAYDRRHKAIKNAAVTMGSERLKA
                     AEARDASKKRTEELFRNGRITRDRYQQGMDKADDIYKRKVGKAVETYKSTKAAATEQM
                     YDEFIDIRQRNLRQKGKIAKADQEKQNYDDKVRAINSNPSLSNEMKQAALLKAKQDYA
                     GGGSWKNIEANLPILTAWLAGGTGLKKLGQGIEDYHPFRLSADAADLYLKDSNAAQER
                     VKQISQRTDHAALFHDLAAGDFSGGLAGITPKHKNFYEKLTNRSNPAETTAAINNLVG
                     AVGQANPNEKKDQNIIRSMLRGIAGFEFNGGDVSRLQPLIKALNSQAAGTGGKVDQYD
                     DYLKKAVKMRQVGPNAAVGQGDGSLRVNSFASGMHKANIIGVDFDQLKSLGDLTPDVE
                     TGKVVGANIAPEKMAAVASELTGLIDNELATLEQAGSAMVTEPLDVLEQKISQLRDQR
                     STALQNGNLDEASSAMEELEPLSRIRDLRVAKERLADPANLDNLSLKNTGVSLASARE
                     SHTTNIHEALHGFGLDNENLTEAIAQKMASGSYRVDDVAAAAQNIKDIMAQKNITQEQ
                     VTAADIDQAVSGVQPISQAEQVISKENGETVINNQTTINNTTIYNNQEEGDESNNDNN
                     NDEYFDGADKNSREPKKINKLSEDIKQRQRLEDYRYFRGLNEDKNDRRRAENIEKAVK
                     QPPKQ"
     gene            complement(14397..15101)
                     /locus_tag="PKV49_02460"
     CDS             complement(14397..15101)
                     /locus_tag="PKV49_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22503.1"
                     /translation="MAEAAVNLQDQYSSPYYNGEILRQQKAEASQQANNQRISAQVID
                     SSRINDRLMSRQNSNNQDQNSTESNQTSRKETATQDTADSLKQAQQLAKLAANPTVAG
                     AAAVAGEMAIDAIKKGNGGKMLTGEFLKTWWTQLINSYGLTIIPLNLQAWVGFIEGHK
                     FFCKLGEEWKAPGIFKKAIGWLEVAGLLTLDLLIFLIILAVLSFLIFIINVMTNPIEN
                     MKILWDLFGGALDGLL"
BASE COUNT         4479 a         3032 c         2975 g         4835 t
ORIGIN      
        1 ctcgagcaat agcggttcgt tgttgctcac cgccggaaac ttcatgtgga taacgatcca
       61 tctgttcact aaggccgaca attttcatta atggcggaat aatggcattg atttttttcg
      121 atgatttgcc acagacttgc agggcaaaag cgacattttc ggctagagtt tttttgggta
      181 ataatttaaa atcttgaaaa ataacaccga tttgccgccg taaaaagggc acttcgctac
      241 tggacagtct ggtaatatcc caatcgccga cgattatttg accgctatct aaacgctctt
      301 caccgattaa acatttaacc atggtggttt tgccgctgcc tgattggccg acaattgata
      361 caaattcgcc agggccgatg ttgagagaca ggttgtctaa agctttagtt tgcggtgggt
      421 aaaatttact agcttgttta aatttaatca taatttgata atgattaatt caaggcggtt
      481 gttaatattt ggctggcggt ggcgtagctc ttggaccaac ttgatgaacc taaagtaaca
      541 acaataaatt tttgtttagt gttctgattt tgcgccgcca tagctaaaca gtggccggct
      601 tccactaaat aaccggtctt ggaagattgt agtggaaatt ggccattttg taataattta
      661 ttggtatttt tgatagtgtg cggttgctta gtgttggtag tgctaaattt ataaatagcg
      721 gtcgtgctta tttttttaat attagcatcc tgactgaccg cttttaaaat taaagcgtaa
      781 tctttggcag tgctcacatt atcagttgat aggccggtcg gttcaacaaa tttagtatta
      841 atagctcccc atttcttggc attagcgttc attttttgga taaattcggc gcggctaaga
      901 ccactaacac ggactagcga ctccacggcg ttattagctg atccaattaa agcggaatag
      961 actaaatctc taatttttaa ttgatcgccg tctttcagtt ttaaaagagc ggcttcggat
     1021 aacgcgcaat atttggtgat ttcttgttca tcttgacgac gatagctgac tactttattt
     1081 aaattaggtt tagtgtccag gaaagtttta atcgccacca ttttggttaa actagcaatc
     1141 ggcacaattt tttgggcatt tttttgccag agaacttgat tgttatcagc atccaaaaca
     1201 accaccgaat tggcatcttg attggatatt tctaaagact taaaatcact aaccgttttg
     1261 gtggaactgg cggcataggc taaattattg attatttgcc aatcagctga attcttaggt
     1321 aagcggccat attttttggt gaattgttgc aaagcaagtc tttctttagc taaatcgcgg
     1381 gttggttttg gcgattctgg cttgttattg ctggcgctcg aggttgaact tggtgatagc
     1441 ggttccaaac gaacagtaat aacgccagtg cctagtgtag ctaattgagc aaaagcctct
     1501 ttttgtaaat caattactcg atcaggatga gcttgggggt cggggccata atcattaata
     1561 accacatcaa cagttttgcc actggctaga tgataaactc tgactttttg gccaagcgga
     1621 taaatagtag aagcagcaaa gagtcctggt ttgaagttgt accagctagc tttaccgctg
     1681 ggactgctcg ttgccgaaac agcggcatta gctgaggtta aaattggctg agctaaaatt
     1741 aagataaggc cggcgataat aaatagcaat cggccaattt ttaaccaata aaaattagat
     1801 cgtgatgaat ccataagttg acgaatcaaa agctttgtct ttataataaa attatagcac
     1861 atttctgatg agccaccaaa cggatattat gacagtttgg cgggtgtcgt atagtggtta
     1921 ttgtgccagc ttcccaagct ggagagggcg gttcgattcc gctcacccgc tcaggttatc
     1981 gtcgcccgtg tagcttagct ggtagagcaa tcccttcgta agggaaaggt cgtcggttca
     2041 aatccgacca tgggctcaaa cataaaaacg ccaaatggcg tttttattgt actttatgat
     2101 caaaaaaata tttgaaccac aaactaggat tacgactgat cgccaccttt tccttttggg
     2161 gagccggtaa atcaaagacc gaattagcgc tatgatcagc ggcgtcagtt gacgaatcgg
     2221 attgcttgat aacaattatt tgttcattgg gctgacgtaa atcaaatttt aggcgcgcct
     2281 cctcttctaa aaattcttta gaatcaagat aatctaccaa gtcggccacc tcttgatttt
     2341 ttttgtctaa acgcttaatc tcagcttcca gttgagttat ctcgctttta acaccaaaaa
     2401 cttgacgaat atttttaact agtccggtta gtataaaaat taaagccaat aaaccagcaa
     2461 tagtgattaa tattgattgg taacgtttgt gtcgattttt gctaggcagt ttattcatag
     2521 tcgggctatc cggaatcgaa ccggaactac atgcacccca tgcacgcgta ctaccactat
     2581 acgatagccc gttaagcggt tttgaggccg ctttcaatta ttcttcctcc tcttcatcgc
     2641 cttcgccttc agcataaatc gcttgcaggt caagacgatc ccaatcaaat aattcttctg
     2701 gttgaaagaa tctagccagt tctttgtgag cattgtcaac gctatccgaa gcatggacaa
     2761 cattagcttg gatactcatg gaaaaatcgc cgcgaatagt gccggcatca gctttgcggc
     2821 ccaaagtttc gccagtcagt aaacgaacac tattaactgc ctccatacct tctagggcca
     2881 taacaataac tggccaatca gacataaatt tagcaatcct tgggaaaaat ggcttgtcga
     2941 tcaaatgaga atagtgctca gctaagatag cgttatctaa ttgcatcatt ttgaggccga
     3001 ctatttttag gcctttgcgt tcaaagcgat ggataatttc gccagccaaa ctacgattta
     3061 gagcgtctgg cttgataatt accagagtgc gctcctgtct tttctttgcc atatatcttc
     3121 aataattccg cctaacaata gcggttggtt attattataa accactaaag cagttttgcc
     3181 aaattagtta ttagcgataa ttattgccag attaattgac cattgcttaa aatattaaaa
     3241 tcgcttaatt gatcagtgcg ccataggcgt ataccgagac gacgcagacg atcaataata
     3301 agttggctag gtagtccaaa tttattggtg ctggccaaac tgataaccgc tattttgggt
     3361 tgccaatagt ttaaccaggc caaactagtg gcggttaacg aaccatggtg ggcaattttt
     3421 agaatatcaa tcggttggcc gatgggttgt tgggctaata attcgctttc agcatcagcc
     3481 tccaaatcac cagtccagag gaatttattg tggccgtatt gccataatat aactaaagac
     3541 tggttattaa tattatcttg gtagcgaatc aattgttcat tggttggcca caagatggtt
     3601 agctggctat tatcggataa ttttatgact gctggcgctc ggacatcatt aataataatc
     3661 cccttttgtt gagcatcatt taagacatta gctaattcgg ttgataattg cggcggttga
     3721 gccaaaatta tctgctgaat cggatattta ttaattaaat aaggcagacc gccccaatga
     3781 tcagcatgat catggctgat aataattatg tctaaatgtc tatcccacca tcgtagttta
     3841 cggctaagcg ctgataaaat ttgttggttt ggcccacagt caattaaaat gttttggccg
     3901 ctggtggtag taattaaggc ggcatcgcct tggccgatgt ccagatagct tattcgcagc
     3961 caagacgata ctgatgattt agtagtggcg attattatta tgatcaagct gattaaaccg
     4021 ccaagagcca accagataaa taattttttc atattaatga gctaaggcta atcccctaat
     4081 ctcgttggct ccagactgct taagacaatt agccatttca gttaaagtgg tgccagtagt
     4141 gacaacatcg tcaataataa taaaattttg gccagctgaa tattttaacc aagaaaaggc
     4201 ttgatcaaga ttggttaatc gtgcgttttt gttcaaatgt gcttggggcg gccggtgttg
     4261 acggcgccaa tagctagcag ttggacagtt attgacttta gctatggtct caaccagtaa
     4321 ttgagcctga ttaaaaccgc gccaattaag ccgctgccga ctcaaaggaa cagccgtaat
     4381 aagcgattgc ttaaaccata attgctcagc aatggtttgc tgaataaatt ccgctaaatt
     4441 gatagctaaa atccggccag ctatggttaa ccgttggtat ttagttaatt taagcagctt
     4501 atttaagagc ggctgatgat aatcagcaca ataaataatc gcggttagac ttgatttatg
     4561 gtcaattggt aacgaccgcc aatgccaatt agctgttaat tgttgttgac cgtcatgaca
     4621 cagccagcta cccggctgct gacaatggta gcagtgtttt ggccataata aatctaacca
     4681 aaaaagccca tgacgggctt tttgttcccc tcctaacata aattaaaaaa ttaattattg
     4741 atattggcga tcttgccaag tcagtttatt ggctagccaa acatcatttt gtttaactaa
     4801 acttaattga tagttttgat aaaaattttt gggttgagtt gaagccgtag cgatagtttc
     4861 ttggccacga atcttaacat caacagttag tgaagtgcta tcaaccgaat ctaaagttcc
     4921 ttgagtaaca atagcttgag ccctggtgcc gatataagta tcagtctttt tagtggctga
     4981 ttgatttttt atataatcat cggtccaagc aatcattgat tgagtcatgc tatttttgag
     5041 aatttccaaa ttgctaaaat tatcttggct agatagacta gcccagcgtt ccacaaaggc
     5101 gccagctttt ttctctagtt caatctctag acggccgcga ttatctagaa ttatttggtt
     5161 attagccagt tgttcggtag aaaaagtttg cggctctggt tgggttattt caggggtggt
     5221 tgtggtcgga ctgacgatgg ttgcaggcgg attataactg ggattcagct tgccgctaag
     5281 tagaatatac agcaaaacaa tcaaaataat aataacccca ccaatcatta gccaattaat
     5341 agtttttgga gaaagccgca taaagttaat tagttaatta tctattcagc ttcagcctga
     5401 gcttcagcct gattttgttt ttccatcttt aataattgtt ccggatcgga agttattaat
     5461 tgatcttcta tataagaggc gacaatttta atggcggcat gtttgttgcc ggcaaaaaat
     5521 attccctcac caacaccgct ttccaataat agatattttt ctccttctgt taatataaaa
     5581 gttttttgca ccagatcaat ggcggctgga gactgtttta ataatatttg catggccgag
     5641 ttggtgacaa tagctttacc gtatggtgat aatagaaaat cattaacatc ttgagtaatg
     5701 gtggtaacgc ctaaataata tttacggcaa cgtttaacta gggcataaat aaatttggcg
     5761 ctatcttcat tctgcattaa ccaccaggct tcgtcaatta ctaaaatgcg tttttttaat
     5821 tggctgcgga ccacattcca aatataatta actatggtat agatagctaa aggccgcaac
     5881 tcatcttcaa gatcgcgaac cgagaaagcg actaattgat tgtccatttg gacattagtt
     5941 ggactagtaa ataatccgga aaaagtgccc tcagtatatt ttttgagccg taacgcaata
     6001 ttctcaccgc cttccattcc ttctaaaata tactggaaat cccgcatagt tggcggttca
     6061 atctggctta aatcactatc ggcggtaata tctttcttgg cataagtttc aaggagagcc
     6121 atatcaataa tagaatcttc ttcggctgat aattggccca acataattct aactaagccc
     6181 ttaaccgtaa taacgccgct ccgaataata tcctcagttt tggattcttg tccgaccggt
     6241 cgtggcaaat caaatggatt aattttggcc tcgctagcta acgatatatt aatataggtg
     6301 ccgccaacag cttcagctag ctctttatat tcatattccg gatcaataac aataacatcg
     6361 gtgcccatca tcattgtccg gagaacctct agtttaatcg cataactttt accggcgccg
     6421 gatgtagcaa aaacaacatt attagcgttg ggcagactga aacgatcaaa tagaattaaa
     6481 ctattattgt gacgattgat accgtaaaga ataccgttat cgctagtcag ttcactggaa
     6541 ataaagggaa aagacgaggc aaccggtgat gaattcatat taaaagcaat atttaaacca
     6601 tcatcacaca gtggcaaggt ggaattaaaa ccttgttcag cttgataaaa aactttttta
     6661 gaatagataa gtcgtgaacc aaataaatct tctatgttat tggctaattg gttgagctct
     6721 tcttcggttt tggcgtaaat agtaacatac agagcgaatt gaaaaaattt ttcagtccct
     6781 tgagttaagg aatcacgcag agcctcaaca tcgcgcaaag cagtttctct taatggatca
     6841 cgcggattac cgcgatcaat gtcagcgcta atttgagctt ctaagacacc agccttattt
     6901 tttaactgtt tcaggacaat agccgaatta attggataaa agaacatgga gatgtcaaac
     6961 gattcgttaa gattgagaat cggcgcaaac cagccaacac tgatatagcg aggataagca
     7021 acaataaaaa tagtgctaac atattggccg ttgagcaata aatatttagg tttaacctca
     7081 agactagatg gcgctatcaa atcacgaata ttgacgacgc cataacgata ggctctttct
     7141 tgttcaatca actcctcggc caatctggct tgttcagcaa gagcggccga agtctgttta
     7201 tttaaaaaac taatatcgtt tgctttgtcg ggactggttg tttttttgtt aggctgaata
     7261 ttgaacatgg cggaatttaa aaattattat tgatattaat tttttcaacg ttatcaaggg
     7321 gttggttagc ggcggtttct tggttataga cattgtaaaa catttcaatt aagccctggg
     7381 tgtctaattg aacgacatta agccccatgg atgataagcc gttaataacg ctgacaattc
     7441 tttcggaaag ttgttttttt aaacggataa agcgttcttc tttgagatta ataacatcaa
     7501 tcggccggaa agcctcaatg agcgattgcc aaaacccttt gcgtttgtcc gaaattggat
     7561 tataaggaac aacgatataa aaatgcttgt tcattatctt gccaagacta actaactgac
     7621 taacataatc aatatattgc aatgtttgat tctttaataa ttcattagtt tgttcattgg
     7681 cttttttgcg cattgactct aggtagttgg cgatattaag ttcgcgtgat tggactacca
     7741 cttggatagg aaaatcaaga ttattaagaa aactgacata accgctaata attgcctctt
     7801 gctcatcttc gcttttaagg gcgaaattaa tgctagatac cattaggatg gcccgtaacg
     7861 tgccgtcttt cataataacg gtatcttttt taatatcagc tatctttaaa tagcgctgag
     7921 tagaaattgg attgtttttt tgctgactag ccataactaa ggcttagtaa ttaactcatc
     7981 aatcaattct gtctctccgc cataagcacc gcgcgtatca acaattagtg ataactcagc
     8041 taaacgtgaa gaagtaaaac gatttttaac agctggcggc tgtgctttaa taatattttt
     8101 gctatcaaat tgagcttcag ctaaattctt gccaaaattt ttattccaaa gacgtagccg
     8161 cggcataaat aaactctgaa tcagattaag taaaaaataa tgaaacggtc ggccgttaat
     8221 cttgccaaaa gcaaaagtaa aaaatacacc ggcagttaat agagtggcta ctaaaaacag
     8281 agagaagtca aacagtttat attctaaaaa aattaccccg gacatgacta atagtataat
     8341 aaattgccga gtggtaatcg gaccgataat tttatcttcg gcgtcaataa attgtgggac
     8401 agtaaattgt tgcataatta aaagcggatc tgtttattta aattcattat agcgataaat
     8461 tcgttgaaag ttaaattatc attatccgga ttgtcttctt tggatagtaa ttgttcggct
     8521 ggttggcaat tttgaatgct acgctggcca atttgcagat ataggcggtt aagcggactt
     8581 tgtcgccaag cgctaagacc ttgcagacgt ttgctataac cgtgctcagc taacatatct
     8641 agcttttcct tgatcttttg ggtgcgttct tctggactac ggccaagata acgaaagcgt
     8701 tttaaattga gattggctaa ctcatcaatc ggggtcaagg ttttaggggt aaaacggaca
     8761 tcgtccatgc gaattttacc ggttttatta tcagtcgcgc gttgatcaat tggaacaaca
     8821 attgtcggcg ctgaatcagt tgatacagct gcaacttgct gatcgtcggt tgaatttttt
     8881 tctgccggcc gggcggcttc cagtaacttg gaattattag ctggtggtgc tggtagctca
     8941 ctttcggtct ttagatctgg tcgtggccgg ttatcaatct ttttaagact agctaataaa
     9001 tcagactcaa aatcacgaac agccgtttga ttaattaact gatcaactgg cttgactggc
     9061 ggcgcgacag ctacttgttg ctcagccgcc cgatgaagca gttctttagc ctcaattaat
     9121 tgttgattga gctgttgctt ttgattggct gacaccccta acccgccgct agttaaagga
     9181 cgagacaaaa aatcatccac ggctcgttga ttacggacac cgattaaaaa ggttttagcc
     9241 aattgtcgcc aacggtttat tagttcgggt tgtttaaata ttttttgttg actaactaat
     9301 ttgttcaaga ccgattccag atcaatatta actgtcgatg acgggtcagc tttttgatta
     9361 agcggcggct gactggtagc tgttaaggct ttaagcgtgc cgcccttagc tagctgattg
     9421 atatcaatcg gcgcgtttaa tggtttagct aaatagcttt ggacagttac taatattttg
     9481 gcttgtaatt cggcagttaa tttttgcgct tgatcgccag ataaaccata attttcacta
     9541 agataataag ataaacgact tggcgatatg tctttaacca tgattttaat taatagactc
     9601 gccaaagaaa gacgataggt ttcttctaac tgcttaatgg ttgccctaat taagggggtg
     9661 tccagcttag cccgaacatc agccgggact tgttgatatt tttgcaaata atcaaacata
     9721 ctttaatttt ttttcagctt aataatctcc tcagtaatcg cctgtgcttc caaactgtcg
     9781 gggttataac gcttaagcat cagctgcagc tgattgattt tattaataac aatttcgtct
     9841 tttggtgccg ctgcactttt ggttggcgct actagcggct gcatttcggg atcaattgat
     9901 tgagcagtgg tatgactggt agtggaatca ctagttaaat caatagtttc gtcaaaggaa
     9961 acattttttt ccggtagtgg gatgatagcc caatcagtac cgtcatcgtt aggcatacta
    10021 tcggggaaaa acttaatatt acgataagta ttaaaaagag cacgtaagat tttcttgtct
    10081 gaatcggaaa gattggtagt gttttttgac aaccccaaga actcggaaat gcttaaagag
    10141 ttaaaatttt cagccccttt ttcggcagcg aaacaacgca gccaattagt gactgtgcct
    10201 ggaaattttt cgttattgat aactaacgac tggtcagtga ctttttcttg attttgtaat
    10261 aaggcagttt caatttgttt acgagcgctt aaatcacgat ctagccaagc aaatatccgc
    10321 tgattgatgg cggcaataat ttgttcgtaa ttttttaact caactagtaa attttctttg
    10381 aacaagttaa taattattga tttttcttcg tctaacgtcg gactaatttt atggggatcc
    10441 tgattaaaaa aggataagcg cttaatgatt ggctcaaaat aaagactgtt cagcttattg
    10501 gttattgtcg cagcgattct ttcaggaaga tcatagcgct tcattaaata ctcggttaaa
    10561 ttatccaatg ataactcatt aatagctatc agaattacta gaaagcttaa ttccaaacca
    10621 tattcagcgg ctaattcatc aattattttt aaacggtctg gatgattaaa ttctagaatt
    10681 acctcatcag gcaattcata aaatttatcg agacagctgt ataaatattg tttagtagac
    10741 atagtgttta ttgcttaggg ggctgtttaa ctgctttttc aatattctca gctcggcgtc
    10801 tatcattttt atcttcgtta agaccgcgaa aatagcgata gtcctccaat ctctgtcttt
    10861 gtttaatatc ttcggacaat ttgtttattt tttttggctc tctgctgttt ttatcagccc
    10921 cgtcaaaata ttcgtcgtta ttattgtcat tattagattc atcaccttct tcttggttgt
    10981 tgtagatcgt agtattatta attgttgttt gattattgat aacagtttcg ccgttctctt
    11041 tggaaataac ttgttcagct tgactaatcg gttgaacacc actaacggct tgatcgatgt
    11101 cggcggcggt tacttgttct tgggtaatat ttttttgtgc cataatatct ttgatatttt
    11161 gcgctgctgc tgcgacatca tcaactcgat aagaaccgct agccattttt tgagcaatgg
    11221 cctcggttag attttcatta tccaagccga aaccatgtag cgcttcatga atattagtag
    11281 tatgactttc acgagcacta gctaaagaaa cgccggtatt tttaagactt aaattatcta
    11341 aattagccgg atcggctaaa cgttctttag caacacgtaa atcacgaatt cttgataaag
    11401 gctctagttc ttccatggct gaactagctt catctagatt gccattttgc agagcagttg
    11461 atcgttgatc gcgcaactga ctaatctttt gttccaggac atcaagcggt tcagtcacca
    11521 tagccgaccc agcttgttct aatgtagcta attcattatc aattaaacca gttaactcac
    11581 tagcgactgc cgccattttt tccggagcaa tattagcgcc aaccacctta cctgtttcaa
    11641 cgtctggggt taaatcacca agcgatttca actgatcaaa gtcaacacca ataatattgg
    11701 ctttatgcat gccgctagca aaagaattaa ctcttaatga tccatcacct tggccgacag
    11761 ccgcatttgg accaacttgg cgcattttaa cggccttttt taaataatca tcgtattggt
    11821 ctacctttcc accagttcca gcagcttgac tatttaaagc tttaattaaa ggttgcaatc
    11881 gactaacatc accgccatta aattcaaacc cggcaatacc gcgtaacatt gatcttataa
    11941 tattttgatc ttttttctca ttggggttag cttgaccaac agcgccaacc agattattta
    12001 tggccgcagt agtttcagct ggatttgatc tgttagttaa cttctcataa aaatttttgt
    12061 gtttaggggt aataccagct aaacctcccg aaaaatcacc agcggcaaga tcgtggaata
    12121 aggcggcatg gtcagttctt tgtgatatct gcttgactct ttcctgagcc gcattactat
    12181 cttttaaata taggtcagct gcatctgctg ataatcgaaa aggatgataa tcttcaatac
    12241 cttgaccaag ctttttaagt ccagtgccac cagccaacca agcagttagt attggcaaat
    12301 tagcctcgat atttttccat gagccaccgc cagcataatc ttgtttagct tttaatagag
    12361 cagcttgctt catttcatta gataaactgg ggttactatt tattgcacga actttgtcat
    12421 catagttttg tttttcttgg tcagctttag ctattttacc tttttgtcgc aaatttcttt
    12481 gtcttatatc tataaattca tcatacatct gttcagtagc ggctgcctta gtagatttat
    12541 aagtttcaac agcttttccg acttttcttt tataaatatc atcagccttg tccatgcctt
    12601 gttgatagcg atctctagtg attctaccat ttctaaacaa ttcttcagtt ctcttttttg
    12661 aagcgtctct agcttcagct gcttttaacc gctcgcttcc cattgtgacc gctgcgttct
    12721 taatggcttt atgccgccta tcatatgcat cttcataggc tggcactgta gtgccgccaa
    12781 tcattctttt aataccgccg ccaatcactt tattaagagg agattgagca agtccagcgg
    12841 cagtagagcc gccaatcatt aatgcgccca gaaaagggtt gccggtaagt atcccggcag
    12901 ctgtgccgcc agccatggtt aagccaccct taacagtttt accgattgtt ttaacaacgt
    12961 catcattagc aacctcctta aaagcagcca ttgttttgtc gccgacacca agtccaagtt
    13021 tttttagagt cttgagacgt tttttagtct tttcttcttt acgttctttg gttaattcat
    13081 caccccaagt tttgccggcg gtgcccaatt tttgaaacat ttggccaact ttgctattac
    13141 tatttttact catggcataa gttaagccgc cagctccctt taaagcattg cggcccgccc
    13201 atttggctgt ggtggtggcc attccaccgg ctgttttttt aactttattg cccattttct
    13261 taagattgct catgccggcc ccgacaacgc cacccacaag agagctggtt tctttagtca
    13321 acatcatacc accccataat aaaccgatgg acactataaa cttaataatc gcttgagctg
    13381 tgccagattt agttacccca gcattaatat catccgtcaa atcagagcct tgattgggat
    13441 caatcgcttg gccactgcca ccagtaagtt ttatctcatt gtccttattt actgaattac
    13501 tgacaccgct gcccgaagct gtaaaaaatg acaaccaaat aaagaaagcc agcatcgggc
    13561 cagtaattac cagtccagta aaatcagacc accagcgact gccggcttgg cctaacttac
    13621 ctgctttggg caaagcggat aataaataag gaaaaggcga caaaacaata taaatccata
    13681 acatgacggc tcgctgaatt aacattatag ccattgttaa aataactact aaagcaacaa
    13741 taatatatat tagggccagc agataactaa taaaaacacc taaaccctcc aatggtgcat
    13801 ctgtggcctg gacactatta ttaacaacaa catcaaataa agcagttaac ccgagcatct
    13861 cagttaaatt accagcaccg acatccttaa aaccgttaac aaaagttaac atgataattt
    13921 gggcaaaatc caagattagg ccgcagatag ttttggaaaa attaacaaaa atggcaccaa
    13981 gaataaaact acgcaataaa ttcttagctt ggtaattctg caatcttaaa accgtgccaa
    14041 aagcgataat taaaagacct aagacaaaga acatattgca gacatcgcga acaactttcc
    14101 acccctcaac cactacctgc tcattaataa aattattata ttgggcaaca tagactagaa
    14161 catgcaagac aacggctaat aacttgccaa aaaaagatac caaaatacta ataatgccgg
    14221 ttaatatttt taaaatggct gtcgtgataa tatcaagggc aaaaacaaaa attggagtaa
    14281 aaattagtaa taaaattaaa ccggcaacaa aatatttttg tttcggacta agttggcccc
    14341 agctattgct aaaaaaagtt tttatttttc gccacatata taagtgttgt catgaactaa
    14401 agtaggccat ctagcgcgcc accaaataaa tcccaaagaa ttttcatatt ttctatggga
    14461 ttggtcataa cattgataat aaaaattaaa aaagataaga cggctaaaat aataagaaaa
    14521 attaataaat ctagggttaa tagtccggcg acctctagcc agccaatagc ttttttaaaa
    14581 attccgggag ctttccattc ctcgcctaat ttacaaaaaa atttatgccc ttcaataaaa
    14641 ccaacccagg cctgcaaatt tagagggatt attgttaggc catatgagtt tattagttgg
    14701 gtccaccaag tttttaaaaa ttcaccagtg agcattttac cgccattacc ctttttaata
    14761 gcatcaattg ccatttcgcc agccacagcg gcggcaccgg ctacggttgg attagctgcc
    14821 aatttagcta attgttgtgc ttgtttaagt gaatcggcag tgtcttgggt ggctgtctcc
    14881 ttacggctag tctggttaga ttcggttgaa ttttggtctt gattattaga attctgacga
    14941 gacattaatc gatcattaat cctggaagaa tcaattactt gagcacttat tctttggtta
    15001 ttagcctgct ggctagcctc agctttttgt tgacgtaaaa tttctccatt ataataagga
    15061 gaagaatatt gatcttgcag attaacagct gcttcagcca taatttcaag ccattttaaa
    15121 ttgacttaat tataccataa tcaaaaaaac cgagcaaacg actgatgatt tatttgccga
    15181 ttaatctaaa gccaccctaa ttagggtggc tttagattaa ctaattcgtt tggctgggtt
    15241 taattctaaa cgagaaactt gatgttgcgc cccagttatc agtcaaaata atggtagcat
    15301 tattggtctg gcattcattg c
//
LOCUS       DAOPAI010000015        14882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_4069_length_14882_cov_4.760909, whole genome shotgun sequence.
ACCESSION   DAOPAI010000015 DAOPAI010000000
VERSION     DAOPAI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4069_length_14882_cov_4.760909"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..309
                     /gene="rplQ"
                     /locus_tag="PKV49_02465"
     CDS             19..309
                     /gene="rplQ"
                     /locus_tag="PKV49_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPL22504.1"
                     /translation="MLRNLASSVLMHEFVVTTKAKAKAVQPMVEHLLTIAKVGDLTAR
                     RSLMAVLPQAEAVTKALSLAERYQSRNGGYTRLTNLGPRQGDGAEQVRLELV"
     gene            320..670
                     /gene="rplM"
                     /locus_tag="PKV49_02470"
     CDS             320..670
                     /gene="rplM"
                     /locus_tag="PKV49_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPL22505.1"
                     /translation="MMEREIHEIDATGQAIGRLATQIATLLRGKNQATYQPNIDAGAV
                     VHVHNITKVIFTGNKLDQKNYYHYSGYQGGLKTLKMRDLAASQPDQVLRRAVKQMLPA
                     NRLRAGMLKRLIIN"
     gene            683..1174
                     /gene="rpsI"
                     /locus_tag="PKV49_02475"
     CDS             683..1174
                     /gene="rpsI"
                     /locus_tag="PKV49_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPL22506.1"
                     /translation="MEELDILEQENSQSKAPAKITDDSVDYTLTREFSGKYITAVGKR
                     KTATSQVRLYEKGRGIIVINGQALAAYLPILSHQAAVIQPLRVTGLQHSLDFSVLVTG
                     GGKSGQAEAIAHGIARCLVKMNEAVKPVLKASGLMTRDARIKERKKPGLKRARRAPQW
                     SKR"
     gene            1289..2734
                     /locus_tag="PKV49_02480"
     CDS             1289..2734
                     /locus_tag="PKV49_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPL22507.1"
                     /translation="MSTRVTNIARNTSYFTLALILQKIISLTYFTLYARVLGPADLGK
                     YYTAISLTNIFLIFIDVGLSNVLTKEVAKDNSKANAWIGSTIAIKLPLALLTSLVLIG
                     LVNILNFPELTRQLVYLSIVCMVLDSFTLTFFACSRAFHNLKFESLSSIIFQLIVLVG
                     SIVILHHGGGVRWLMLSLIAASLYNFIYSLLILWRYWRVKPWPIWYWPKIKKVWLLAA
                     PFAGYAIAQRAYTYLDSVMLAKIAGDVAVGIYQVPFKIINALQFLPMAFVASLYPALS
                     LYWHNNRSQLSITFTRAIQYSAIISLPIAIGGWVIADKLVLIFSDGYTEAIWPLRLGL
                     AALPFIFIGYPIGSLLNACDRQKRNTYNMLIVLIVSIVANLFLIPRYGVIGASLTVML
                     TSILMVVLGIGQIKSILGQSPQLFSGLLRIVVAASLMGLAVWQAKFINNIFILVAWGG
                     FVYLVALFAVKAWCWSDIVGIFKSFFRQSAD"
     gene            2738..3826
                     /locus_tag="PKV49_02485"
     CDS             2738..3826
                     /locus_tag="PKV49_02485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPL22508.1"
                     /translation="MRYLLVTLEYPPFHGGIAHYYSHLAAAWPRNDEFIVLDNNHNQL
                     QAARGFWPWRRSFQAIWQAIKQQRIDYVLVGQILPLGTVVWLLSYFLPIKYGIFLHGM
                     DWGQAMKRPRKRWLVSRIIARASSVIAANSYVAGLVKQAWPSITNRLIIINPGLSQTT
                     SPTISDTYRQQLRQQYGLVDQKILLTVGRLVERKGVDQVLRALPELINKYPNLYYLVV
                     GQGPYLATCQQLASDLAVNSRVIFITDADDQQRAAWYQLADIFIMPARQLAGDFEGFG
                     IVYLEANQAGKPVIAGRSGGVADAVVDGLNGLMVDPNSSYEIIAAVSRLLDDSAFSQQ
                     LGQAGQARLAAFLWPTLAAKLAQFINKH"
     gene            3836..4543
                     /locus_tag="PKV49_02490"
     CDS             3836..4543
                     /locus_tag="PKV49_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPL22509.1"
                     /translation="MISVIIPVYNQADKLGACLNSLIKQTYRDLEIIIVDDGSTDGLE
                     AVLIEWEPKLQAAKLPYKIIVQTNQGSNPARNRGLAEASGDYLLFSDADICWRSDALA
                     KMLSALETNPQVSYCYSSFRFGWKKFRLWPFDAEKLRRQPYIHTSALVRRQDFPGFDN
                     NIKRLQDWDVWLTMLENDKIGLFYDDFLFTVAGGGYISSWLPSCAYKLLPFLPRVKKY
                     KQAEAILRAKHHLPIYD"
     gene            4536..4997
                     /locus_tag="PKV49_02495"
     CDS             4536..4997
                     /locus_tag="PKV49_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPL22510.1"
                     /translation="MTKCQLIVASLLLIIVILGGVVYWGNRRPVFRGEYKLFSANQKF
                     LGNIDILTDKRQQYRGFSRQDQPCPDCGLLFIWPILSQPTMVMREMNFALDFIWLRDR
                     QIVQFTENAAPEGHNPQIKYRPDQPIDAVLEMPSGFISRHNLQVGQFLNWQ"
     gene            5008..6480
                     /locus_tag="PKV49_02500"
     CDS             5008..6480
                     /locus_tag="PKV49_02500"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPL22511.1"
                     /translation="MQFSWKTVRYILEAIILLELLAALGYFYPVIGTALFIISCLVIL
                     WLTIRRLEWGLYVLVIDLLISSKGYLLSLNLGGFKLSWRMAIWLIIMAVWFVQLIVAI
                     YRRQAWPKQLWQRLKDSGWLILLAMIIFAIINAWWRGHQLANIFFDANGWLFWLIFLP
                     ILTVARWSINQLSSVIVAGLAWLTAKTGFLLYAFGHFSPGLRMVIYRLIRDTQVGEIT
                     SLSGQFYRVFLQSQVYFLIFLVIIAWQLAGRLPLKHWPRWLIGRPGRLYLLAFSLSLL
                     PIIVGFSRSFWFGLVLSMFFLLIMIWRRYRWPVVWRWSLSLLLASVLSIVLIVVIVRF
                     PWPQPQLSFKAGDLLEERLGNLDSEAGASSRWSLLPKMVQAISQSPFFGQGLGATITY
                     RSADPRILENESSGYYTTYAFEWGWLDIIFKFGFLLALWYFIWLGRLAWRSWRYGPVG
                     EALAIGLLVVAAVHFFSPYLNHPLGIGFLAGMLAWLVRQK"
     gene            6482..7561
                     /locus_tag="PKV49_02505"
     CDS             6482..7561
                     /locus_tag="PKV49_02505"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPL22512.1"
                     /translation="MLPYLAASFLAFFLAVVTTPLIRRLAWRLAIIDRPDQIRKLQSQ
                     PVALLGGWAIFIGISLTALIFRRYLIIGDLTYHHWLGVWLGGLILMIGGSLDDRYNLP
                     PHKQLIFPILAVLAVIVGGVGIKKITGLDGQLIFLNSWQISLFSWQGIVRHFVVLVDL
                     FTFFWLMGMMYTTKLLDGVDGLVTGLVGIGSLVIGLFTLTTKYYQPDIALAAFILAAA
                     CAGFLLYNWSPAKIYLGDGGSLMLGYLLGVLAIISGGKIAIALLIMGVPILDVVWVIV
                     RRLFNGQNPLRTADRKHLHQRLVDLGWSAKRTAITYYAVSLVFGLSALFLQSQGKRLA
                     LLLLIILMTIVVIRFSLLDKPKQKP"
     gene            7618..7929
                     /gene="rplU"
                     /locus_tag="PKV49_02510"
     CDS             7618..7929
                     /gene="rplU"
                     /locus_tag="PKV49_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPL22513.1"
                     /translation="MSKFAIIATGGKQYKVRPGQTIKIEKLSSEPAAEVKFDALLLAD
                     EQTQDIQLGRPILNAAITAKVLDHGQEDKITVIKYKNKTRYKKTLGHRQQFTKIEIQD
                     W"
     gene            8048..9241
                     /locus_tag="PKV49_02515"
     CDS             8048..9241
                     /locus_tag="PKV49_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPL22514.1"
                     /translation="MAAKNATVRPVFLTDNFSLFKLVKKLLQKVNPRERDVLLRRFGL
                     NSSNKQTLEQIGASHGLTRERVRQIEAAGLKKIKKMCELEKQIGLLRQALDDLLLDNG
                     GLMDKQYLQSSFLALSREALKDDDLPIYANNFDFLISRVLSEDFDPVTSGLFNDSIKL
                     KYQTIDHLELLAQQLVEQVRQAKRLFLIEDLIKLSFDLDAFKANADKLVSANKLDLAS
                     ALSGQDSFQDGQLINKYKPIYSVLRAIRDIERNNFGSWGPADWPEIRPRTITDKIYLV
                     LKQTDQPLHFREITSRINQFFTDKRANAATVHNELILDDRYVLVGRGIYALKESGYQA
                     GRVVDIVTRILKQARHSLSKDEIIDKVLTQRLVKKTTVSLILSNKQLFQRLPDGTYSL
                     QDKAT"
     gene            9312..10814
                     /locus_tag="PKV49_02520"
     CDS             9312..10814
                     /locus_tag="PKV49_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22515.1"
                     /translation="MEIVLNLAPLLNFFSLPADEMFRRIILYYGWMIPAVIFLYGAGC
                     VWLAWRRRLFFSKQKFVLLAIDVPRANEQTPKALENMLCYLAGAHGTINLIETYWEGK
                     FQLGFSFEIVGIDGYIQFLVRTPVAFRSLVETAIYSQYPDAEIAEVDDYVNTVPSTFP
                     DPDFDMWGAEFIPVQMEPEFKQALPIKTYKDFEYQLGRPEFHFKDPMAPLMDLMSSLR
                     PGEQIWYQLLILPVAADWSKIGKPAIDNVLGIKPKDDPTIVDRFFEWFLELMSQFSEM
                     IYSLWGNIEDKPAKEEKKEINMLNLLPVQKKQIEAIQEKISKMGYGVKLRMIYVSRRE
                     VMNKPKAVNGFVGFIKQFNANDLNALKPDTKVTQTSSNYFYKLSQIIRKKNKVIRAYK
                     DRDDTAGRKLWYLNVEEIASLWHFPIETVVKAPLIQKTTSKKMEPPMALPVEDSLASD
                     LALDDLFNLDDESVDEQPAEAPASSESSAPSVATESHQAPPPSNLPGIND"
     gene            10820..12436
                     /locus_tag="PKV49_02525"
     CDS             10820..12436
                     /locus_tag="PKV49_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HPL22516.1"
                     /translation="MNQEVTLFGETTFRNQGYKFGIKLDDRRRHMYIIGKSGMGKSTI
                     LENMIVQDISKGRGLAVVDPHGDLAEKILEYIPDNRVKDVIYFNPSDAEYPIAFNVVE
                     QVEPHLRHLVASGLIGVFQKLWADSWGPRLEYILRNAILAVLDYPGSTLLAITRMLSD
                     KNFRKKVIDKVQDPVVKAFWVNEFSGYNDKFASEAVAPIQNKVGQFLSSALIRNIVGQ
                     VKSSINIRHIMDEGKILIMNLSKGRIGEDNSALLGSMMITKLQLAAMSRVDTNEEDRR
                     DFYLYVDEFQNFATDSFANILSEARKYHLSLILAHQYIEQLSDVVRAAVFGNVGTLVT
                     FRVGATDAEFLAKEFMPLFTEEDLVNIPKFNFYIKLMIDGLTSDPFSAKTLAPLSKEE
                     RYPNLQAVIEHTRENYASQRDIVEEGIMRWHLNQEDDDVKKVAPTNALADKVEILLNK
                     KEAIQPTSSDLAGPAAKKAAAHVSAEQRYPAVCDGCNQPTTTRFVPDGKRPVYCPECL
                     RRYRAEQSQRQQLASSSPASLQRLKKGHGR"
     gene            12426..14018
                     /locus_tag="PKV49_02530"
     CDS             12426..14018
                     /locus_tag="PKV49_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL22517.1"
                     /translation="MAVDYLKIGRATDLTGWERRFYRFLEIIPGLLSWGTLLTLIILS
                     YFHPVWVALFIIAFDVYWLLLVVYLAIILISAYRRTRKSIVIDWHQRCLSLQPVDLTY
                     QPMIGSAIVSRQVVWSDLYHLVIFPCYNESYEIIQPGLKAIVDSSFPKDKMIFVLAIE
                     ERGGAAIQATADRLKAEYGDNFFRFQIVVHPDNLVGELKGKGANQAWAAQVIRRELID
                     RLGLDYNLLLTSVFDCDTVIYEQYFYCLVYKFLTSDKPYRRSYQPIPVYHNNIWQAPF
                     FARVAAYSNTFWQMMQQIRQEKLSTYSSHSMSWRALEEVGFWSTKMVSEDSRIFWHCF
                     CHYNGDYEVEPMHYPVSMDVCMDQKVLPTARNLYKQQRRWGWGVENVPYLIFNTIKRW
                     KQIPKKTFVNKIFIQMYGFHSWATNALIIGFVGWLPLFIGGDRFNQTVLSNNLPNVTR
                     ILMSIAMIGMVLSAIISSLLLPKRPAGNPWWRRFSMIGQWIFLPITIVFFGAIPGLEA
                     QTRLMLGRYMGFWVTPKERTNS"
     gene            14135..>14882
                     /locus_tag="PKV49_02535"
     CDS             14135..>14882
                     /locus_tag="PKV49_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPL22518.1"
                     /translation="MTDLFLYNTLSRQLELFRPQQPPVVGLYTCGPTVYNYAHLGNLR
                     AYLFADLLKRVLLYNGYNVRHIMNITDVGHLTDDGDAGDDKMEQGAAREHKTAWEIAE
                     FYTKAFQDNLVALNILPPTTYCRATDYIAEQISLAKTLDNKGYLYKTSDGLYFDTSRV
                     ADYNKLSHLPLEQLREGARVDRNPEKRQPTDFAVWKFSPAGQQRQMEWDSPWGKGFPG
                     WHLECSAMSLKLLAGHLDIHCGGIDHINVHH"
BASE COUNT         3727 a         2759 c         3424 g         4972 t
ORIGIN      
        1 aaaggtccgc gtgaaatgat gttgcgcaat ttagcctcta gcgttctgat gcacgaattt
       61 gttgtcacca ccaaggccaa ggccaaggcc gttcagccga tggttgagca tttattaact
      121 atagctaaag ttggtgattt gactgctcgg cgcagtttaa tggctgtttt gccgcaagct
      181 gaagctgtaa ccaaagcctt aagcctagct gaacgctatc agtctagaaa tggcggctat
      241 actcgcctga ccaaccttgg accgcgtcag ggtgatggag ccgagcaggt tcgtcttgaa
      301 ttagtttaag tgtttgttta tgatggaacg agaaattcac gaaattgatg cgaccggaca
      361 agcgattggc cggctagcca ctcagattgc caccttgctg cgcggcaaga atcaggcgac
      421 ttaccagccc aatattgatg ctggcgctgt tgttcatgtc cacaatatta ccaaggttat
      481 ttttactggc aataaattag atcaaaagaa ttattatcat tatagcggct atcaaggtgg
      541 tctcaaaacc ctcaagatgc gtgatttggc ggctagccag ccggatcaag ttttgcgccg
      601 cgccgttaaa cagatgttac cggctaatcg cttgcgtgca ggcatgctca agcgtctgat
      661 cattaattag tctacgatta ttatggaaga attagatatt ttagaacaag aaaacagcca
      721 atcaaaggct ccagccaaga ttactgatga ttcggttgac tatactttga cccgagaatt
      781 tagtggcaag tatattacgg ctgtcggtaa gcgcaaaaca gcgacctctc aggtgcgttt
      841 atatgaaaaa ggccgcggta ttatcgttat taacggccaa gctctagccg cttatttgcc
      901 aattttatct catcaagcgg cagttattca accattgcga gtgactggct tgcagcacag
      961 tttggatttt tcagttttgg tgactggcgg cggtaagtct ggtcaagctg aagcgattgc
     1021 tcacggtatt gcccgttgtt tagtcaaaat gaatgaagca gttaagccag ttctcaaagc
     1081 cagcggtctg atgactcgcg atgctcgcat taaggagcgc aagaagccgg gtctcaaacg
     1141 cgctcgccgc gctccgcagt ggagtaagcg ttaatcagcg ttcttttacc tttctatatt
     1201 atacaaaaac accggttcgc cggtgttttt ttggttgctc actccgctta aatgttgtat
     1261 attgaagata attttaagcc tgttttttat gtctacccgc gttactaata ttgccaggaa
     1321 tacttcttat ttcactttag ccttgatttt acaaaagatc atttctttaa cctattttac
     1381 gctatacgcc agagtcctgg gaccggctga tttgggaaaa tattatacag ctatttcttt
     1441 gacgaatatt tttttgattt ttattgatgt tggtcttagt aatgttttaa ccaaggaagt
     1501 cgctaaagac aatagcaagg ccaacgcttg gattggctcg accatagcca ttaaattgcc
     1561 gttagcattg cttacatcgc tagttttaat tggcttggtt aatattttaa attttcctga
     1621 gcttactcgg caattggttt atttatcaat tgtctgtatg gtgttagatt cctttacttt
     1681 gacttttttt gcctgttcgc gagcttttca taatttgaaa tttgaaagtt tatcgtcaat
     1741 tattttccag ctgattgtac ttgttggcag tattgttatt ttgcaccacg gcggcggcgt
     1801 ccgctggtta atgctgtcgt tgatcgcggc cagtctgtat aattttatct attcattact
     1861 aattctttgg cgttattggc gagtcaagcc ctggccaatt tggtattggc cgaagataaa
     1921 aaaagtttgg ttattagcgg ccccttttgc cggttatgcg attgctcaac gagcctatac
     1981 ttatcttgat tcagttatgc tagccaagat agccggtgat gtggcggttg ggatttacca
     2041 ggtgccgttc aagattatta atgcgttgca attcttaccc atggcctttg tcgcctcgct
     2101 ttatccggcc ctaagtcttt attggcataa caaccgatcg cagctgagta ttacctttac
     2161 tcgagcgata caatattcag ccattatttc tttaccgatt gccattggcg gctgggtgat
     2221 agcggataaa ttagtactga tctttagtga tggttatact gaggctatct ggccgctccg
     2281 tttaggttta gccgccctgc cctttatatt tattggttat ccgattggct cgttactgaa
     2341 tgcttgcgat cggcagaaac gcaacaccta caatatgttg attgtcctga tagttagtat
     2401 tgttgccaat ctctttttaa ttccgcgtta tggtgttatt ggcgctagct taacagtaat
     2461 gctgactagt atcttgatgg tggttttagg tattggccag attaagtcaa ttctcggtca
     2521 gtcgccccag ctattcagcg gcctattgcg cattgtagta gccgctagtt tgatgggctt
     2581 agccgtttgg caagccaagt ttattaataa tatttttatt ttagtggctt ggggcggttt
     2641 tgtgtattta gtagctctat ttgcggttaa agcttggtgt tggtcggata ttgttggtat
     2701 ctttaaatct tttttccgtc agtcagctga ttaatgtatg cgttatttat tagttacctt
     2761 agaatatcca ccctttcatg gcggtatcgc ccattattac agccatctag ctgcggcttg
     2821 gccgcggaat gatgagttta ttgttttgga taataatcac aatcagctcc aggcggctcg
     2881 cggtttttgg ccgtggcggc gatctttcca agctatttgg caagctatta aacagcaacg
     2941 gattgattat gttttagttg gtcaaatttt accgctaggg acggtggttt ggttgttatc
     3001 ttattttttg ccgattaagt atggtatttt tcttcatggt atggattggg gacaggccat
     3061 gaagcggcca cgtaaacgtt ggttggttag ccgaataatt gctcgcgctt catcagttat
     3121 tgccgccaac agttatgttg ccgggttagt taaacaggct tggccgtcaa ttactaatcg
     3181 tttaataatt attaatcccg gtttaagcca gacaacatca ccaactatta gcgatactta
     3241 tcggcaacag ttgcgtcaac agtatggttt ggttgaccaa aaaattttat taactgtcgg
     3301 tcgtttagta gaacgcaaag gagtggatca agttttgcgg gctctgccgg aattgattaa
     3361 taaatatcct aatttgtatt atttagttgt tggccaaggg ccatatttag ctacttgcca
     3421 acaattagcc agtgatttgg cagttaattc acgggttatt tttataactg atgccgatga
     3481 ccagcagcga gcggcttggt atcagctagc cgatattttt attatgccgg cccgtcaatt
     3541 ggctggtgat tttgaaggct ttggtattgt ttatctagag gctaatcaag ctggtaaacc
     3601 agttatcgcc ggtcgatccg gcggtgtcgc cgatgcggtg gttgacggtt taaatggctt
     3661 aatggttgat ccaaattcaa gttatgaaat tatcgccgcg gttagccgac ttctggatga
     3721 ttcggccttt agtcagcaac ttggccaagc cggccaggct cggttagcgg cttttctctg
     3781 gccgaccttg gcggccaaat tggctcaatt tattaataag cattaattat aatttatgat
     3841 ttctgtcatt attccggttt ataatcaggc tgataaatta ggcgcttgtt tgaatagttt
     3901 aatcaagcag acttatcgtg atttggaaat aattattgtt gacgatggct caactgatgg
     3961 tttagaagcg gttttgatcg agtgggaacc taaattgcaa gccgctaaat tgccatataa
     4021 aatcattgtt caaactaatc aaggatccaa cccggctcgt aatcggggtc tggccgaagc
     4081 tagtggtgac tatctgttat ttagtgatgc tgatatttgt tggcgttctg atgccttagc
     4141 taaaatgttg tcagccttgg aaactaatcc tcaagttagt tattgttatt cttcgtttcg
     4201 tttcggttgg aaaaaatttc gtttatggcc gtttgatgct gaaaaattac gtcggcagcc
     4261 ctatatccac acttcagcct tagtgcgccg tcaagatttt cccggttttg acaacaatat
     4321 taaacgtctt caggattggg atgtttggtt gactatgtta gaaaacgaca aaattggttt
     4381 attctatgat gatttcttat ttaccgtggc cggcggcggt tatatcagta gctggttacc
     4441 cagttgtgcc tacaaattat tgccgttttt accacgggtt aaaaagtata agcaggccga
     4501 agctattttg cgagccaagc atcatttgcc aatttatgac taaatgtcaa ttaatagtag
     4561 ctagtttgtt gttgatcatt gttattttgg gcggagtggt ttattggggc aaccgtcggc
     4621 cggtttttcg cggcgagtat aaattatttt cggccaacca gaaattttta ggcaatattg
     4681 acatcttaac tgataagcgc cagcaatatc gcggttttag ccgccaagat caaccttgtc
     4741 ccgattgtgg cctgcttttt atttggccga ttttatcgca gccaaccatg gtgatgcgcg
     4801 agatgaattt tgctttagat tttatttggc ttcgcgatcg gcaaatagtt caatttactg
     4861 agaatgctgc cccggaaggc cataatcctc agattaagta tcggcctgat cagccgattg
     4921 acgctgtctt agaaatgccg agcggtttta ttagccgcca taatctacag gttggtcaat
     4981 ttttaaattg gcaataatta gcaaattatg caattttctt ggaaaacagt gcgctatatt
     5041 ttggaggcta ttatcttatt agagttgttg gccgctttag gatattttta tcccgtcatc
     5101 ggaacggctc tttttattat tagttgcttg gttattcttt ggctgaccat ccgtcgactg
     5161 gaatggggct tgtatgtctt agttattgat ttactaatca gctctaaagg ttatttatta
     5221 tcattaaatt tgggcggttt taagttgtcg tggcgaatgg ctatctggct gattattatg
     5281 gcggtttggt ttgttcaatt gattgtggct atctatcgcc gtcaagcctg gccaaaacaa
     5341 ttatggcaac gtcttaaaga tagcggttgg ttgattttgt tggccatgat aatttttgcc
     5401 atcattaatg cttggtggcg cggtcatcaa ttggctaata ttttttttga tgccaatggc
     5461 tggttatttt ggctgatatt tttaccgata ttaactgttg ctcgctggtc aattaatcaa
     5521 ttaagttcgg tgatagtagc tggcttagct tggttaacgg ctaaaaccgg ttttttactg
     5581 tatgcctttg gtcatttttc gccaggctta agaatggtaa tctatcgcct gattcgtgat
     5641 acacaagttg gtgaaataac tagtttatcc gggcagtttt atcgggtttt tcttcagtca
     5701 caggtttatt ttttgatttt tctagttatt atcgcttggc aattggctgg ccggctgccc
     5761 ttgaaacatt ggcctcgctg gttaattggt cggccaggtc ggctttattt attagcgttt
     5821 agtttaagtt tattgccaat tattgtcgga ttttctcgta gtttttggtt cggtttggtg
     5881 ttaagcatgt tttttttgtt gataatgatt tggcggcgct accgctggcc agtggtttgg
     5941 cggtggtctt taagtttgtt attggctagt gttttgtcaa tagttttaat cgtggttatc
     6001 gtccgctttc cttggccgca accgcagttg tcattcaagg ccggtgattt gctggaagag
     6061 cggttaggta atttagatag tgaggcgggc gcttcatcgc gctggtcatt attacccaaa
     6121 atggtgcaag ctattagtca gagcccattt tttggtcagg gattgggcgc caccatcact
     6181 tatcgttcag ctgatccgcg aattttagaa aatgagagca gtggttatta tactacctat
     6241 gcttttgaat ggggttggct ggatattatt tttaaattcg gtttcttact ggctttgtgg
     6301 tattttatat ggttgggacg attagcttgg cggtcttggc gctatggtcc ggttggcgaa
     6361 gcgttagcta tcggtttatt ggttgtagcc gcggtgcatt tttttagtcc ttatttaaat
     6421 catcctttgg gcatcggttt tttagccgga atgcttgctt ggttagttag acaaaaataa
     6481 tatgttgcct tatttggctg ccagtttttt agcttttttc ttagccgttg tcaccactcc
     6541 gttgattagg cggttagctt ggcggttagc tattattgat cggccagatc aaattcgtaa
     6601 gttacagagt cagccggtag ctcttctggg cggctgggcg atatttatcg gtatttcatt
     6661 gaccgccttg atttttcggc gctatttaat aattggtgat ttaacttatc atcattggct
     6721 aggcgtttgg ttgggcggcc tgattttgat gattggcggc tctttggatg atcgctataa
     6781 tttgccgccg cataagcaat taatttttcc aattttggcc gttttagcgg taattgttgg
     6841 tggcgttggc attaaaaaaa ttactggatt agatggtcaa ttaatctttt tgaatagttg
     6901 gcaaattagt ttattcagct ggcaggggat tgtccgtcat tttgtcgttc tggttgattt
     6961 atttaccttt ttctggttga tgggcatgat gtataccact aaacttttgg atggagttga
     7021 tggcttggtt accggtttag tcgggattgg cagcctggtg attggcttat ttactttgac
     7081 caccaaatat tatcagcctg atattgcttt ggccgctttt attttggcgg cggcttgcgc
     7141 tggttttttg ttatataatt ggtcaccagc taagatttat ttaggtgatg gcggctcttt
     7201 aatgcttggc tatctattgg gcgtgcttgc tattatttcc ggtggtaaaa ttgccatcgc
     7261 cttgctgatt atgggggtgc cgatcttaga cgtggtttgg gttattgttc gtcgtctatt
     7321 taatggacag aatccgcttc ggaccgctga ccgcaaacat ctacaccagc ggttagttga
     7381 tttaggctgg agtgctaaac gaacggcgat tacttattat gctgtttctt tagtttttgg
     7441 cttaagtgct ttatttttac agagccaagg caagcgccta gctcttttat tgctaattat
     7501 attaatgact atcgtggtta ttcgtttttc attacttgac aagcctaagc aaaaaccgta
     7561 atataatgac acacctgtcc cgtaaggttt tattttttta atacaatcaa tagcgatatg
     7621 tctaaatttg ctataattgc cactggcggc aaacaatata aagttcgtcc cggtcaaacc
     7681 attaaaattg aaaagttgtc ctcggaacca gccgctgaag tcaagtttga tgccttgcta
     7741 ttagccgatg agcagaccca agatatccag cttggccggc cgatattaaa cgccgcgatt
     7801 actgccaaag ttttagatca tggccaggaa gataagataa ccgttatcaa atacaagaac
     7861 aagactcgct ataagaagac tcttggtcat cgtcagcaat ttaccaagat tgaaattcaa
     7921 gattggtagt tattattaaa acaacccatc attagcgggt tgttttattt tacgctagct
     7981 attgacttat ttacaaaaag atgttaaata tataagtaat attttatcta aatagcttga
     8041 tttgtctatg gctgctaaaa atgctaccgt taggccggtt tttttaactg ataatttttc
     8101 tttatttaaa ttagttaaaa aattattaca aaaagttaat ccacgcgagc gtgatgtctt
     8161 attgcgccgt tttggcttaa attcgtcaaa taaacaaacg ctagagcaga ttggtgccag
     8221 tcatggcctg accagagagc gagttcgtca aattgaggcg gctggtctta agaaaatcaa
     8281 aaaaatgtgc gaattggaga agcaaattgg tttattgcgt caagccttgg atgatttatt
     8341 gttggataat ggcgggttga tggataagca atatttgcaa agcagttttt tagcgcttag
     8401 tcgggaagcg ctcaaagatg atgatttacc aatttatgct aacaattttg attttttaat
     8461 ttctcgggtt ttgtctgaag attttgatcc ggttacttcc ggcttattta atgattcaat
     8521 taaactaaaa tatcaaacga ttgatcactt ggaactgttg gctcagcaat tagtggagca
     8581 ggttcgtcag gccaaaagat tatttttaat cgaagattta attaaattga gttttgattt
     8641 agatgctttt aaggctaatg ctgataaatt agtgagcgcc aacaagttag acttagctag
     8701 tgctttatct ggtcaggata gctttcaaga cggtcagttg attaataaat acaagccaat
     8761 atattcagtt ttacgggcta ttcgcgatat tgaacgcaat aattttggtt cttgggggcc
     8821 ggctgattgg ccagagattc gtccacggac gattactgat aagatttatt tggtgcttaa
     8881 acagactgac cagccattgc attttcgcga aattaccagt cgaattaatc agttttttac
     8941 tgacaagcga gccaatgccg ccacggttca taatgagttg attcttgatg accgttatgt
     9001 cctggtcggt cgcggcattt atgctctcaa agaatccggc tatcaggctg gtcgcgtagt
     9061 tgatattgtt actcgcatct taaagcaagc tcgccattct ttatccaagg atgagattat
     9121 tgataaagtt ttaacacaac gcttggtcaa aaagactacc gttagcttaa tcttatctaa
     9181 taaacagctg tttcagcgtt tgcccgatgg cacatattcc ttacaagaca aggcgactta
     9241 atcattattg gttgtctttt cttttgctat atcttatgat aaactggaag taatctccag
     9301 tttatttgtc tatggaaata gttttaaatc tagcaccatt attaaatttt tttagtttgc
     9361 cagctgacga aatgtttcgg cggattatat tgtattatgg ctggatgatt ccggcggtaa
     9421 tttttttata tggcgcgggt tgcgtctggc tggcttggcg tcgtcgcctc ttttttagca
     9481 aacaaaaatt cgtactttta gccattgatg ttcctcgggc taacgagcag acgcccaagg
     9541 ctttggagaa tatgttgtgc tatctagccg gcgctcatgg cactattaat ttaattgaaa
     9601 cttattggga aggtaaattc caactcggtt tcagttttga aatcgtcggt attgacggtt
     9661 atattcaatt tttagtgcga acaccagttg cttttcgcag tttagtcgaa actgccattt
     9721 attcgcaata tccagatgcg gaaattgctg aggttgatga ttatgtcaat actgttccca
     9781 gcacttttcc tgaccctgat tttgacatgt ggggagcgga gttcatccca gtccaaatgg
     9841 agccggagtt caaacaagct ttaccaatta aaacttataa ggattttgaa tatcaattag
     9901 gtcggccgga atttcatttt aaagatccga tggcaccgct gatggatcta atgagttcct
     9961 tgcggcctgg cgagcagatt tggtatcagc tgctgatatt accagtggcg gccgattggt
    10021 ctaaaattgg caaaccagcc attgataatg tgctaggtat taaacctaag gatgatccga
    10081 ccattgttga ccgttttttt gagtggtttt tagagttaat gagtcaattc agtgaaatga
    10141 tttacagtct ttggggaaat attgaagata agccagccaa agaagaaaaa aaagaaatca
    10201 acatgctcaa cttattgccg gtgcaaaaaa agcagattga agctatccaa gaaaaaatat
    10261 ccaagatggg ctatggtgtt aaattgcgaa tgatttatgt gtctcgccgt gaggttatga
    10321 ataagcctaa ggcggttaat ggttttgttg gttttattaa gcagtttaat gctaatgatt
    10381 tgaatgcttt aaaaccggat actaaagtca cgcaaacatc ttctaactat ttttataaac
    10441 ttagtcagat tatccgtaaa aagaataagg ttataagagc ttataaagac cgcgacgaca
    10501 cagctggtcg caagctgtgg tatttaaatg ttgaagaaat tgcttcgctc tggcattttc
    10561 cgattgaaac agtagttaag gcgccgttaa tccaaaagac aaccagcaag aagatggagc
    10621 caccaatggc tttgccagtg gaagattcgc tagccagtga tttggccttg gatgatttat
    10681 ttaacttaga tgatgagtca gttgatgaac agccagctga agcaccagcg tccagtgaat
    10741 caagcgcgcc gtcggtggcc acagaaagtc atcaggcgcc gccgcctagt aatttaccag
    10801 gtattaacga ttgaaatata tgaatcaaga agttactttg ttcggagaga caacttttcg
    10861 caatcagggt tataaatttg gcatcaagct agatgatcgc cgtcggcaca tgtatattat
    10921 cggtaaatcc ggcatgggta agtcaaccat cttggaaaat atgattgtcc aagacattag
    10981 caagggccgc ggattggcgg tggttgatcc gcatggtgac ttggctgaga aaattttaga
    11041 gtatattcct gataatcgag ttaaagatgt tatctatttc aatccgtcgg atgcggaata
    11101 tccgattgct ttcaacgtgg tggaacaggt tgagccgcac ctgcgtcatt tggttgcttc
    11161 agggctgatt ggtgtttttc aaaaattatg ggctgattct tggggaccac gcttggaata
    11221 tattttgcgc aatgctatct tggcggtttt agattatcct ggctccaccc tattggccat
    11281 tactcggatg ttatccgata agaattttcg taagaaggtg attgataagg tccaggatcc
    11341 ggtggttaaa gcattttggg tcaatgaatt cagtggttac aatgacaaat ttgcctcaga
    11401 ggcggtagcg ccgattcaaa ataaggtcgg tcagttttta tccagcgcgt tgattcgtaa
    11461 tattgtcggc caagttaagt cttcaattaa tattcgtcac atcatggatg aaggcaagat
    11521 tttaatcatg aatctaagta agggacggat tggtgaagac aattctgccc tgcttggttc
    11581 aatgatgatt accaagctac agctggcagc catgagccga gttgacacca atgaggaaga
    11641 ccgacgcgat ttttatctct atgttgatga atttcagaat tttgctactg atagttttgc
    11701 caatatttta tctgaagcgc gcaagtatca tttaagttta atattggctc atcaatacat
    11761 tgaacagctt agcgatgtgg tgcgggccgc tgtttttggt aatgtcggca ccttagttac
    11821 tttccgtgtc ggcgccactg atgctgaatt tttggctaaa gaattcatgc cattatttac
    11881 tgaagaagat ttagttaata ttcccaagtt caacttttat atcaagctga tgattgacgg
    11941 attaacctct gatccttttt ccgctaagac tttagccccg ctcagcaaag aagaacgtta
    12001 tcccaattta caagccgtta ttgagcatac tcgtgaaaat tatgctagtc aacgcgatat
    12061 tgttgaagag gggattatgc gttggcatct caatcaagaa gatgatgatg ttaagaaggt
    12121 agcgccgact aacgccttgg ccgataaagt tgaaatttta ctaaataaaa aagaagcaat
    12181 tcaaccaacc tcgtcagact tagctgggcc agcagccaag aaggcggctg cccatgtttc
    12241 agctgaacag cgttatccgg ctgtttgcga tggttgcaat cagccgacga ccactagatt
    12301 tgtgcccgat ggtaaacggc cggtttattg cccagagtgt ctgcgtcgct atcgggctga
    12361 gcagagccag cgacagcaat tggccagttc atcaccagct tcactgcaac gattaaaaaa
    12421 aggtcatggc cgttgattat ttaaaaatcg gtcgagctac tgatttaact ggctgggagc
    12481 ggcggttcta tcgtttttta gagattattc ccggtttatt atcttggggg actttgttga
    12541 ccttaattat cttgtcttat tttcatccgg tttgggtggc tttgtttatt atcgcctttg
    12601 atgtttattg gttgctttta gttgtctatt tagccattat tttaatatca gcctatcgcc
    12661 gtactagaaa gagtattgtt attgattggc atcagcgatg tttatcgctt cagccggttg
    12721 atttgactta tcagccgatg attggttcag cgattgttag tcggcaagtg gtttggtcag
    12781 atttatatca tttagtgatt tttccttgtt ataatgaaag ttatgagatt attcaaccgg
    12841 ggctaaaggc gattgttgat agttctttcc ctaaagataa aatgattttt gtcttagcta
    12901 ttgaagagcg tggtggagcc gctattcaag cgacagctga ccggcttaaa gcggaatatg
    12961 gcgataattt ttttcgtttt caaatcgttg ttcatccgga taacttagtt ggcgaactca
    13021 agggcaaggg cgccaatcag gcctgggctg ctcaggttat tcgtcgagaa ttaattgacc
    13081 ggctcggact tgattataat ttattattaa ccagtgtttt tgattgcgat accgttattt
    13141 atgaacaata tttttattgt ttggtttaca aatttttaac ctcggataaa ccctaccgcc
    13201 gtagttacca gccgattccg gtttaccata acaatatctg gcaagcgccg ttttttgcta
    13261 gagtggctgc ttattcgaat actttttggc agatgatgca gcagattcgc caagagaaat
    13321 tatctactta ctcatcgcac tccatgtctt ggcgggcgtt ggaggaagtc ggtttctggt
    13381 caaccaagat ggttagcgaa gattcgcgga tattttggca ttgtttttgc cactataatg
    13441 gcgattatga agtggagccg atgcattatc cagtatccat ggatgtttgt atggatcaaa
    13501 aagtattacc gacggcgcgt aatctttata aacaacaacg acgctggggt tggggagtag
    13561 aaaatgtgcc ttatttgatt tttaatacta ttaaacgctg gaagcagatt ccgaaaaaaa
    13621 cttttgttaa caagattttt atccaaatgt atggctttca ttcctgggca accaacgccc
    13681 tgattattgg ttttgtcggt tggctgccgt tatttatcgg tggcgaccgt tttaatcaaa
    13741 ctgttttatc taataattta ccaaatgtaa ctagaatttt aatgtcaatt gccatgatcg
    13801 gcatggtctt gtcggcgatt atttcttcat tactattgcc gaaacggcca gccggcaatc
    13861 cttggtggcg gcgctttagt atgatcggcc agtggatttt tttgccgatt acgattgttt
    13921 tctttggtgc tattcctggc ctagaagcac aaaccagatt gatgctaggc cggtatatgg
    13981 gtttttgggt aactcctaag gaacggacta attcttaatt ttactatatg ccttttattg
    14041 accctgatcg ctctggaacc acatttttaa gaatttttgc tattgagttt ttgggcattg
    14101 ccattgtttt tggcttgctg gcgatttttt agctatgact gatttgtttc tttataatac
    14161 tctcagccgt cagctagagc tctttcggcc gcaacagcca ccagtcgtcg gcctctatac
    14221 ttgcggtccg acagtttaca actatgccca tttgggcaat ctgcgcgctt atctttttgc
    14281 tgatttacta aaaagagttt tactatataa tggctataat gttcggcata taatgaatat
    14341 taccgatgtt ggccacttaa ctgatgatgg cgacgccggc gatgacaaga tggaacaagg
    14401 ggcggcccgt gagcacaaga cggcttggga aatcgctgaa ttttatacta aagcttttca
    14461 agacaatctc gttgctctca atattttacc gccgactact tattgccgag ctaccgatta
    14521 tatcgctgaa cagattagtt tagctaagac tttggataat aaagggtatt tatataagac
    14581 cagcgatggc ctttattttg atactagccg agtggctgat tataataaat taagtcattt
    14641 gccattagag caattacgtg agggagcgcg agtggatagg aatccagaaa aacgccaacc
    14701 aactgatttc gcagtctgga agtttagtcc agcgggacag caacgacaga tggagtggga
    14761 ttcgccctgg ggcaagggtt ttcccgggtg gcatttggag tgttcagcga tgagtttaaa
    14821 gctattagct ggacatctag atattcattg cggcggtatt gaccatatta atgttcatca
    14881 ta
//
LOCUS       DAOPAI010000016        13298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_4654_length_13298_cov_4.179416, whole genome shotgun sequence.
ACCESSION   DAOPAI010000016 DAOPAI010000000
VERSION     DAOPAI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4654_length_13298_cov_4.179416"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKV49_02540"
     CDS             complement(<1..361)
                     /locus_tag="PKV49_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPL22519.1"
                     /translation="MWQPIDNQTADEFVRQYGQSELLQSANWLDLQQQFYQVERLGYY
                     HQQKLVAVAGLIRRRLIGRYAYYYSPRGPIWADDQLSSNWWPQMLADIKPWLVARQAI
                     FWRIEPNCQPTGVDLNKL"
     gene            complement(365..940)
                     /gene="ruvA"
                     /locus_tag="PKV49_02545"
     CDS             complement(365..940)
                     /gene="ruvA"
                     /locus_tag="PKV49_02545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPL22520.1"
                     /translation="MLAFLRGQVLTKRPGSLIIDNNGLGYQVFVGERFWEAVASGQSI
                     EVYLYHSVREDAADLYGFASLDELSLFELLVSVSGIGPKSALGILSLANVEQIKQAII
                     LGDESLLTKVSGIGAKTAQRLILELSGKIAGLLPTTSLDGGQAAVSSDDLEALIALGY
                     SASDARRALSKVDAGISDPAQRLKSALQYLH"
     gene            complement(944..1894)
                     /locus_tag="PKV49_02550"
     CDS             complement(944..1894)
                     /locus_tag="PKV49_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HPL22521.1"
                     /translation="MTTAEIYQLAIKLGIEADLRGQASVREHLRYVKKQYRQLSKTAK
                     VDFDLEKLTNPYADSRILVDLTRGQIKKVLAGIDMEGPELLAAKQLGDIDLVIAHHPE
                     GLGLAGLSEVMDLQADVLANYGVPINIAESVIKPRISEVSRGVSPINHDRAVDMAKLL
                     GLDFICVHTPADNLGARYLVDLIKNKQAKLKRVGDLVKLLSTIPEYASAAKHKAGPTL
                     FAGQADNHCGKIAVTEFTGGTSGSKDIYEKMVNFGIGTVVGMHMSEEHRREAEKNHIN
                     VVIAGHMASDSLGMNLLLDQLEKVGIEVVATSGLIRIKRN"
     gene            complement(1898..2203)
                     /locus_tag="PKV49_02555"
     CDS             complement(1898..2203)
                     /locus_tag="PKV49_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22522.1"
                     /translation="MFKFQTPKIDNDFSQLTGDSLQQAWRGEAKQQRWRRTYWSLAVV
                     IVVIMIGGLLIISWPSFQHSFSHWSAWFKDKTGQTVNNLQQWQLKNERSVIEGNILK"
     gene            complement(2207..3115)
                     /locus_tag="PKV49_02560"
     CDS             complement(2207..3115)
                     /locus_tag="PKV49_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPL22523.1"
                     /translation="MNFYQQVDSNRHKTWLLISLSASLLILVGFVFSQVNNNPIWLYI
                     ALAYAVISSFISYWWSDKIVLTISRAKEVQASDSPQLYRLIENLCITAGLPMPKIYVI
                     DDSAPNAFATGRDSKHAAVAFTSGLLAKLDKAELEGVIAHELSHIGNRDILLATIVSV
                     LVGTVVLLADWFARWSFWGGGRRRQDDQGAGQLQAILSIVAIILAILAPLFAQIIQMA
                     ISRRREFLADANSALLTRYPEGLIRALQKISADPEPLEAANRATAHLYFASPFHNKRG
                     VSGLTKMFMSHPPVEERIAALRQINI"
     gene            complement(3127..3693)
                     /locus_tag="PKV49_02565"
     CDS             complement(3127..3693)
                     /locus_tag="PKV49_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPL22524.1"
                     /translation="MTWIIYALMALVAIIVIWLIAVYNGLITSKNRLAEAWSDIDVQL
                     KRRYDLIPNLVETVKGYATHEQETLEKVIEARNQAIAAQNSGDLVDQQQAENNLSGLL
                     RHLFALSENYPDLKANQNFLELQRELTDTEDKIQAARRFYNGNVRDFNTKLQLFPTNI
                     FAKKLGFSPAEFFEIKDDSQRETVKVNF"
     gene            complement(3717..6662)
                     /gene="ileS"
                     /locus_tag="PKV49_02570"
     CDS             complement(3717..6662)
                     /gene="ileS"
                     /locus_tag="PKV49_02570"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPL22525.1"
                     /translation="MDQSSLFFAQSELEVLAFWDQAQIFQQTISKKAPQGEYRFYDGP
                     PFATGTPHYGHIVASIIKDVVPRFWTMKGYRVNRRWGWDCHGLPIENIVEKELGYQHK
                     KDIEALGIDKFNELCRSKVLSYVTDWQKIIHRLGRFVDMDNAYRTMDLSFMESVWWVF
                     KQLWDKGLIYQGYRSMHVCPRCETTLSQSEIADGYQDIKDLSCIAKFKLLPGQKIGDS
                     AVEDNTYILAWTTTPWTLIGNVALAVGAEIKYSKIIVDQQQYIIATERLADVFQDQVL
                     EIIEEYKGSQLVGLRYEPLFDAYYNQPDLVDKDNGWQVYAADFVNTEDGTGIVHIAPA
                     FGEDDLSLGQQYHLPFVQHVAMDGTIKPEAGQLAGLNVKPKDDHSSTDVEIIKYLASH
                     GQLFAKEKYQHSYPHCWRCDTPLINYATSSWFVDITKLKPALFEEAKKINWSPAHIKD
                     GRFGNWLEGARDWSISRQRYWASVIPLWQCSCGWQQVFGSASELAAASGQEITDLHKH
                     IVDKITVPCPQCGGQAQRVPDVLDTWFDSGSMPYAQAHYPFSNPDDFLTNYPARFIAE
                     GIDQTRCWFYYLHVIGTGLGFGAAYQNVIVNGIVLAEDGKKMSKRLQNYPDPGLLLEK
                     YGADALRCYLLSSPVTQAENLNFSEKGVEEKVRQIILPVWNVYKFYDLYAGDNRRILS
                     ISDWLSSNILDRWIVSRLHQLLAEINQQMDAYNLPKATRPLADFIDDLSSWYLRRSRD
                     RFKEKNNNDKAAALLTTGYVLQGLAIALAPFAPFLAEAIWQKTTGYNYQHANQSVHLL
                     SWPDDLPVDQASLLEMTAIRQIAELALAQRDLAGLKVRQVLASLTVSANETKLAAISQ
                     SGWSILADELNVKQIIQQPTATDDWQISLNTELTPELICEGLARELVRNINLLRKQAG
                     LSLQDRTIIYWQGDSGLAEAAFREWQDLIARETLSQSIVQGWLADGQIIAKDLSLADG
                     QIKLALIKF"
     gene            complement(6730..7674)
                     /locus_tag="PKV49_02575"
     CDS             complement(6730..7674)
                     /locus_tag="PKV49_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPL22526.1"
                     /translation="MLNYFRPKRGFTLVELLVVIAIIALLSTLSVVALNSARSKARDA
                     RRLNDLRQISTALAMYHNSKGIYPAIPAEYTSSSITGLCLSNIGISSTCGNLVYLNNI
                     PSDPRGNNIDYQYRQLNNGESFSLVATLENQSADYPNRDVIAGPHGLTTDLLTTHGID
                     WRDINNWDCSGADISYDASLDALKIKKYASCWLGYGGNSIPIDTSAKYYLSAEYMTTN
                     DENKKFSLGTVDYKGSNRIHNSNNFFVARYVPTTNNNWVKVNNSEAHTGESMPPPETN
                     TYWRTGTTYTAIRFYANEPNSVNQNQITYIRNLRFYHQ"
     gene            7743..9566
                     /gene="uvrB"
                     /locus_tag="PKV49_02580"
     CDS             7743..9566
                     /gene="uvrB"
                     /locus_tag="PKV49_02580"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPL22527.1"
                     /translation="MKFQLVANFQPTGDQPQAITALVNGWRQNYRQQTLLGVTGSGKT
                     FTIANVIAQIQQPTLIISPNKTLAAQLHKEFKAFFPNNAVHYFVSYYDYYQPEAYLPP
                     TDTYIEKEATINENIDRLRHAATQALMTRPDVIIVASVSCIYGLGEKLDYQSSSQSLT
                     QGQTINRQTIIKKLLEMQYQRQDMACRRGQFRVAGDTIDIYLATGEAIIRLDLFDDII
                     NAIYLWPINPEIKLPPASLADLNTNEAQKLTEITILPAKHFMTPSDRMTEALTAIRQE
                     LTEQIVQLKKDNKLLEAERLERKTNYDLEMLEQVGYCNGIENYSRHLSGRPAGSPPAT
                     LLDFFPKDFLTIIDESHVAVPQINGMFAGDRARKEVLVEFGFRLPSALDNRPLNFSEF
                     NNKVGAIIYTSATPGRYELNNSQQIVEQVIRPTGLLDPQIEVRPSHGQIADVIAECQK
                     RSIIQERVLITTLTKKMAEELADYLTESGLKTSYLHSDIDTLERVNILQDLRGGRYDV
                     LVGVNLLREGLDLPEVSLIIILDADMSGFLRNETSLIQTIGRAARHINGQVIMYADQI
                     TPAMDYAIKETQRRRQIQADYNQQHGITPTSITNANWSNLD"
     gene            complement(9572..10174)
                     /locus_tag="PKV49_02585"
     CDS             complement(9572..10174)
                     /locus_tag="PKV49_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22528.1"
                     /translation="MNKRLIWILLTAFVLANVLAGIKVARRYQSTTDTDNQSSPMPVA
                     APDFPSPVDLPPAATVTKQDIAKIVCPKPLSAADWRTAIVTDSPAAIDWSGYQLSDSE
                     RQAINQSYQRQAVNLAGHFHLASWSCGHDCHKAAIINIKNGRIIALGLDDDLQTKYGW
                     QFSATSSLLTINPDSQDSSGPTIYALVEDSGLRRICYLAQ"
     gene            complement(10180..10539)
                     /locus_tag="PKV49_02590"
     CDS             complement(10180..10539)
                     /locus_tag="PKV49_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22529.1"
                     /translation="MKTAQEIFNQLQKLKNERKTLNAVYRDALANSSEYQQLTEELKI
                     IRDKRQMVIQAISSDLRSELDRLDDLKGEMANDKALLADAVINQLTAGQPVVVIDEYQ
                     EKYEPVISVSFRKEGNN"
     gene            10615..10851
                     /locus_tag="PKV49_02595"
     CDS             10615..10851
                     /locus_tag="PKV49_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22530.1"
                     /translation="MAKIIRYSRITLAYICLLLGVAGLVLPILPGWIFMLVGLELLIR
                     YQPTNNWAPRIKNRYQGWANKWQQWRSPTNYNNN"
     gene            complement(10871..12673)
                     /gene="thrS"
                     /locus_tag="PKV49_02600"
     CDS             complement(10871..12673)
                     /gene="thrS"
                     /locus_tag="PKV49_02600"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPL22531.1"
                     /translation="MPDYPLEVIRHSLAHILAAAVTELYPNVRPAIGPAIDNGFYYDF
                     DFGPDKLSEDQLPLLNQKMLELIAADLPFNQEIKSAVDALAAEQAAGQTYKAEIIADL
                     QAQGETEVSYYTVGQFADLCRGPHVASTADLRQAGFLVHHLAGAYWRGSEKNKMLTRV
                     YALAFNNQVELDNYLQLLAEAEQRDHRKLGRELDLFHIDEQVGLGLPLWHPKGAILWK
                     VIEDFWSNQHLRNGYSLVRSPHIGNKTLWETSGHWGFYNASMYPPLEAGQTLEERQRG
                     VKVKESEEYLLKPMNCPFHIEIYQNKPRSYRELPLRYAECGTVYRYEKKGELSGLTRV
                     RGFTQDDAHIICRADQVENELKRVVDFILFIFKSFGFDTDLISVYLSLRDPENKSKYA
                     GSDEGWEFTEQVLKKVAIEKKLNFKEEKGEAAFYGPKLDFKLKDALGREWQCSTLQFD
                     FNLPERFTMNYTNSQGQAEQPYVLHRALFGSFERFIALLIENYAGVFPLWLSPVQVKI
                     LTVNDQHHDYCQQLADKLRYNDIRVEVDESDETVGNKIRKAATEKIPYVLVVGDKEIA
                     SGQLAVRQRGQTELLNIDQDGFLQLLQEKIKQQQ"
BASE COUNT         4168 a         2911 c         2472 g         3747 t
ORIGIN      
        1 caagcttatt caaatctact ccggttggct gacaattagg ctcaatccgc caaaaaatag
       61 cttgtctggc cactaaccat ggcttaatat cggccagcat ctgcggccac caattggacg
      121 ataattgatc atcggcccaa attggtccac gcggtgaata gtaataagca tatcggccga
      181 tcagccgtcg tctgattaga ccagcaacgg ccactaattt ttgctggtgg taatagccaa
      241 ggcgttcaac ttgataaaat tgttgctgca aatcaagcca attagcgctt tgtaataatt
      301 cggattgacc atattgccga acaaattcat cagccgtttg attatcaatc ggttgccaca
      361 taaactaatg aagatattgt aaggctgact taaggcgctg agccggatca ctaatgccgg
      421 catccacctt agataaagcg cgccgagcat cagaagcgct ataacctaaa gcaattaagg
      481 cttccaaatc gtcactgctg acagctgctt gaccaccatc taaactagta gtcggtaaaa
      541 gaccggctat tttaccactt aattctaaaa tcaatctttg agccgtctta gcgccaatac
      601 cagacacttt agtcaataat gattcatcac ctagtataat agcttgctta atttgttcaa
      661 cattggctaa tgacaatatc cccagggctg atttaggacc gataccggaa acggaaacta
      721 ataattcaaa taacgacagc tcatcaagac tagcaaaacc ataaagatcg gcagcgtctt
      781 cacgaacgct atgataaaga taaacttcaa tggactgacc actggccacc gcctcccaaa
      841 atctttcgcc gacaaaaacc tgataaccca aaccattatt atcaataatt aacgatccgg
      901 gtcgcttagt taaaacttgg ccgcgcaaaa aagctagcat aaattaattc cgcttaattc
      961 taattaatcc cgaggtcgct acaacctcaa tgccgacttt ttctaattga tcaagcagca
     1021 gattcatacc caaagaatca ctagccatat ggccggcaat aacgacatta atatgatttt
     1081 tttcggcctc gcggcgatgt tcttcactca tatgcatccc gactactgta ccaatgccaa
     1141 aattgaccat tttttcataa atatccttgg aaccactggt gccaccggta aattcagtta
     1201 cggcaatctt gccgcaatgg ttatcggctt ggccggcaaa taaagtcggg ccagccttat
     1261 gtttggcagc tgaagcgtat tcaggaatag ttgataatag cttaaccaaa tcaccaactc
     1321 tttttaactt agcctgctta tttttaatta aatcaaccaa ataacgagcg cccagattat
     1381 cggccggagt atgaacacaa ataaagtcta gtcccagtag tttggccata tcgactgctc
     1441 gatcatgatt gatcggagaa acaccacggc tgacttcgct aattcgcggc ttaataaccg
     1501 attcggcaat attaattggc acaccataat tagctaaaac atcagcttgt aaatccataa
     1561 cttctgacaa gccagccaat cccaaacctt caggatgatg agcgataacc aggtcaatat
     1621 cacctagttg tttagcggct aataattccg gcccctccat gtcaataccg gccaagactt
     1681 ttttaatttg accgcgcgtt aaatcaacta aaatacggct atcagcataa ggattggtta
     1741 gtttttctaa atcaaaatca actttagctg ttttagatag ctgccgatat tgttttttaa
     1801 cataacgcaa gtgctctctg actgacgctt gtccgcgtaa atctgcttca atacctagct
     1861 taatcgccaa ttgataaatt tcggctgtag tcataaatta ttttaaaata ttgccttcaa
     1921 tcactgatcg ctcatttttt aattgccact gctgtaaatt attcacagtt tgcccggttt
     1981 tatctttaaa ccaggccgac caatgagaaa aactatgctg aaaagatggc cagctgataa
     2041 tcaataaacc gccaatcatt ataacgacaa taacaacagc caacgaccaa taagttctcc
     2101 gccagcgctg ctgcttagcc tcgccgcgcc aagcctgttg caaactatcc ccggttagct
     2161 gagaaaaatc attatcaatc ttgggcgttt gaaatttaaa catagattag atattaatct
     2221 gtcttagagc ggcaattctt tcttcaaccg gcggatggct cataaacatt ttagtcaagc
     2281 cggaaacacc gcgcttatta tgaaaaggtg aagcaaaata taaatgagcc gtagcccgat
     2341 tagcagcctc cagcggctct gggtcagcgc taattttttg taaggcgcga ataagtccct
     2401 caggatagcg agtcaataaa gcgctgttag catcagccaa aaattctcgt cgtcgcgaaa
     2461 tcgccatttg aattatttgc gcaaagagcg gggccagaat agctaaaatt atggcaacaa
     2521 tagataaaat tgcttgcaat tgtccagcgc cctgatcatc ttgccgtcgc cggccgccac
     2581 cccaaaatga ccaacgagca aaccaatcag ccaataaaac aacggtgcca actaaaactg
     2641 aaacaatagt agctaataga atgtcccggt tgccaatatg cgacaattcg tgggctatta
     2701 ctccttccag ctcggccttg tctagcttag ctaataatcc gctagtaaaa gcgacagccg
     2761 cgtgtttgga atcgcgacca gtggcaaaag cattgggggc gctatcgtca atgacataaa
     2821 ttttaggcat tggcaagcca gcggtaatac aaagattctc gattaggcga taaagctgtg
     2881 gcgaatcgct ggcctgaact tctttagctc ggcttatagt taaaactatt ttatcactcc
     2941 accaataact aataaaacta gaaatcacgg cataggccaa agcaatatat agccagattg
     3001 gattattatt aacctgacta aaaacaaaac cgaccaaaat aagcaagcta gcacttaaac
     3061 tgatcaacaa ccaagtttta tggcggttac tgtcaacttg ctgataaaaa ttcatagcga
     3121 ggacaactag aaattaacct taactgtttc tcgttggcta tcgtctttaa tttcaaaaaa
     3181 ttcagccggg ctaaagccca gttttttggc aaaaatattg gtggggaata attgtaattt
     3241 ggtgttgaaa tcgcgaacat tgccattata aaaacgacgc gccgcttgaa ttttgtcttc
     3301 agtgtcagtt aattctcgtt gcaattctaa aaaattttga ttagctttta aatctggata
     3361 attttcgctt aaagcaaaca gatgtcttaa tagcccggag agattatttt cggcttgttg
     3421 ttgatcaact agatcaccgc tattctgggc ggctatagcc tgattgcgag cttcaatcac
     3481 tttttctagt gtttcttgtt catgggtggc atagccctta actgtctcta ccagattagg
     3541 aattaggtcg tagcggcgct tgagctggac atcaatatcg ctccaagctt cagctaaacg
     3601 atttttacta gtaatcaagc cattataaac ggcaatgagc cagattacga taatggcgac
     3661 caaagccatt aaagcataaa taatccaagt cataaatttg tttaattata aaatatctaa
     3721 aatttaatta aggctagttt gatttgaccg tcggccaatg ataaatcttt agcaattatt
     3781 tgaccgtcgg ccagccaacc ttgaacaatt gattgactta atgtttcgcg ggcaatcagg
     3841 tcttgccatt ctctaaaagc ggcctcagcc aaaccactat cgccctgcca ataaataata
     3901 gtcctatctt gtaaactaag accggcttgt ttgcgcaata agttgatatt gcgcactaat
     3961 tcgcgagcca gtccctcaca aatcaattct ggcgttagct cagtattaag actaatttgc
     4021 caatcgtcag ttgctgttgg ttgttgaatt atttgcttga catttaactc atcagctagt
     4081 attgaccaac cgctctgact aatagcggct aatttagttt cattagcgct gactgttaat
     4141 gaggctaaaa cttgacgcac tttcaggccg gccaaatcac gctgagctaa ggccagctcc
     4201 gcgatctggc ggatggcggt catttccagc aagctggcct gatcaactgg taaatcatcc
     4261 ggccaagaca ataaatgaac tgattgattg gcgtgttgat aattataacc ggttgttttt
     4321 tgccaaatag cttcggccaa aaatggggca aatggtgcca acgctatggc caggccttgc
     4381 aagacatagc cagtggttaa taaagccgcc gctttgtcat tattattttt ttctttaaag
     4441 cgatcacgcg atcggcgcag ataccaagac gacaaatcat caatgaagtc agctagcggc
     4501 cgcgttgctt tcggtaaatt ataagcatcc atttgctgat taatttcagc taataattga
     4561 tgcaggcgag aaacaatcca gcgatctaaa atattagatg acaaccaatc actaattgac
     4621 aaaattcgcc tattatcgcc ggcatacaaa tcataaaact tataaacatt ccaaactggc
     4681 aaaataattt gtcgaacttt ttcttcaact cctttttcgc taaaattcaa attttccgct
     4741 tgagtaactg gtgatgataa taaataacaa cgcaaagcat cggcgccata tttttctaac
     4801 aataatccgg gatcgggata attctgcagc cgtttggaca tttttttacc atcttcagct
     4861 aaaacaatac cattaacaat aacattttga taagcagcgc caaaaccaag accagtaccg
     4921 ataacatgta aataataaaa ccaacagcga gtctgatcaa tgccctcagc aataaagcga
     4981 gcgggataat tagttaaaaa atcatcggga ttactaaagg gatagtgcgc ttgagcatag
     5041 ggcatagagc cgctatcaaa ccaagtatct aaaacatcag gcactcgttg agcttgaccg
     5101 ccacattggg gacagggcac agtaatttta tcaacaatat gtttatgtaa atcagtaatc
     5161 tcttgaccgc tagcggcagc taattcactg gcggagccaa aaacttgttg ccaaccacaa
     5221 gaacattgcc atagcggtat tactgaagcc caataacgct gacggctaat tgaccaatcg
     5281 cgggcaccct ccagccaatt accaaaacga ccgtctttaa tatgagctgg cgaccaatta
     5341 atttttttag cctcttcaaa caaggctggc tttaatttag taatatcaac aaaccatgat
     5401 gaagtggcat aattgattaa tggagtatcg cagcgccagc aatgcggata actgtgttga
     5461 tatttttctt tagcgaaaag ttggccgtga ctggcaagat atttaataat ttccacatca
     5521 gttgaactat ggtcatcttt gggcttaaca ttaagaccag ctaattggcc agcttcaggc
     5581 ttgatagtgc cgtccatggc tacatgttga acaaacggca aatgatattg ttgaccaaga
     5641 gataaatcat cttcaccaaa agctggcgca atatgaacga tgccggtgcc gtcttcagtg
     5701 ttgacaaaat cagcggcata aacctgccag ccattatctt tatcgactaa atcaggctga
     5761 ttatagtaag cgtcaaacaa cggctcataa cgaagaccaa ctagttgaga acccttatat
     5821 tcttcaataa tttccaaaac ctgatcttga aaaacatcag ctaaacgctc ggtggcaata
     5881 atatactgtt gttgatcaac tatgatttta ctatatttaa tctccgctcc aacagccaag
     5941 gcgacattgc caatcagtgt ccatggcgtg gtagtccaag ctaagatata ggtattatcc
     6001 tcaaccgctg aatcgccaat cttttgaccc ggcaataatt taaatttcgc gatacaagac
     6061 aaatctttaa tgtcttgata gccatcagct atttctgatt gcgacaatgt tgtttcacaa
     6121 cgcggacaaa catgcattga acgataaccc tgataaatca accctttatc ccacagctgt
     6181 ttaaagaccc accaaactga ttccatgaat gataaatcca tggtacgata agcattatcc
     6241 atatcaacaa aacgaccgag acgatggata atcttttgcc aatcagtcac atagctcaaa
     6301 accttggaac ggcaaagctc attaaattta tcgatgccaa gcgcttcaat atctttctta
     6361 tgttgatagc ccaattcttt ttcaacaata ttttcaatcg gcaaaccatg gcaatcccaa
     6421 ccccagcggc gattaacccg atagcctttc atagtccaaa aacgcggcac aacatccttg
     6481 atgatactgg caactatgtg gccataatgc ggcgtgccag tggcaaaagg cggaccgtca
     6541 taaaaacgat attcaccttg cggcgccttt ttactaatgg tctgttgaaa tatttgtgct
     6601 tggtcccaaa aagctaaaac ttctaattcg ctttgcgcaa aaaataagga cgactggtcc
     6661 ataaaataat tcaattataa ctgcctaact ctttaatttt actggaaaat aaagggattg
     6721 tcaaaaaacc tattgatgat agaaacgtaa attgcggata taagtaattt gattttgatt
     6781 aactgaattg ggttcattgg cgtaaaatct tattgcggta taagtagttc cagttcgcca
     6841 ataagtatta gtttctggag gaggcatact ttcgccagtg tgcgcctcac tattattcac
     6901 ctttacccaa ttattattag tggtgggtac ataacgagca acaaaaaaat tattactatt
     6961 atgtattcta ttgctacctt tataatcaac ggtgcctaga gaaaattttt tattttcatc
     7021 attagtagtc atatattcag cagataaata atatttagcg ctagtatcaa taggaattga
     7081 attgccacca taacctaacc aacaagaagc atacttctta attttgagag catctaagct
     7141 agcatcataa ctaatatcag caccgctaca atcccaatta tttatatccc gccaatcaat
     7201 accatgagta gttaatagat cagtggtgag accatggggc ccagcaataa catcacgatt
     7261 aggatagtca gccgattggt tttctagggt ggcaactaaa ctaaagcttt caccattatt
     7321 aagctgacga tattggtaat caatattatt accacgagga tcgctaggga tattatttaa
     7381 ataaactaaa ttgccacaag tagagctaat gcctatatta ctaagacata gtccggtaat
     7441 tgaagatgag gtatattctg caggaatagc tggatagata ccttttgagt tatgatacat
     7501 tgctaaagct gtgctgattt gacgtagatc atttaatcga cgagcatcac gagctttaga
     7561 tcgagcacta tttagggcga ctaccgatag tgtagacaat aaggcgataa tagctataac
     7621 taccaataac tcaactagag taaaacctcg tttgggacga aaataattaa gcatataaaa
     7681 attgctcttt gctactgatt tgattacaat tacaatataa tacaaaacaa tattttaaac
     7741 aaatgaaatt tcaattagtc gctaattttc aaccaaccgg tgatcaacca caggcaatta
     7801 ccgccttagt taatggctgg cgacaaaatt accgccaaca aactttatta ggtgtaacgg
     7861 gtagtggtaa aacatttacc atagctaatg tcattgccca aattcaacaa ccaaccctga
     7921 tcatttcacc gaataaaact ttggccgcgc aattacataa agaattcaaa gccttttttc
     7981 cgaataacgc cgttcattat tttgtgtctt attatgatta ttatcagcca gaggcttatt
     8041 tgccgccgac tgatacctat attgaaaaag aggctacaat taatgaaaat atcgaccgct
     8101 tgcgtcatgc cgccactcaa gccttaatga cacggcctga tgtcattatt gttgccagtg
     8161 tttcttgtat ttatggtttg ggtgaaaaat tagattatca atcatctagc caatctttga
     8221 cacagggaca aaccattaat cgccaaacga taattaaaaa attactggaa atgcaatatc
     8281 agcgccaaga tatggcttgc cgtcgtggtc aatttcgggt cgccggcgac acgattgata
     8341 tttatttagc cactggtgaa gcgattattc gccttgatct ttttgatgat attattaacg
     8401 ctatttattt atggccaatt aatcctgaga ttaaattacc gccggccagc ttagctgatt
     8461 taaatactaa tgaggcacaa aaattaactg agattaccat tttgccggct aaacatttta
     8521 tgaccccatc agaccgaatg actgaagcat tgacagctat ccgccaagaa ctaactgaac
     8581 aaatagttca gttaaaaaag gacaataaat tgttagaagc ggaaagattg gagcgaaaaa
     8641 ctaattatga tttagaaatg ctcgagcaag tcggttattg taatggtatt gaaaattatt
     8701 cccgccattt atctggacga ccagccggct ccccgccagc cacactgcta gatttctttc
     8761 ccaaagattt tttaacaatt attgatgagt cccatgtggc cgtgccacaa attaatggta
     8821 tgtttgctgg tgatcgcgcc cgtaaagaag ttttggtaga gtttggtttt cgcctaccta
     8881 gcgctcttga taaccgacca ctcaatttta gtgaatttaa taataaagtc ggggcaatta
     8941 tttatactag tgccacgcca ggacgctatg aattaaacaa ctcgcaacaa atagtggaac
     9001 aagttatccg accaaccgga ctattggacc cgcaaattga agttcggccc agccatggcc
     9061 aaatcgctga tgttattgcc gaatgtcaaa aaagatccat tatccaagaa cgagtgctaa
     9121 ttaccacact aaccaagaaa atggctgaag aattagctga ctatctaact gaatctggat
     9181 tgaaaactag ttatttacat agcgatattg acaccttgga acgagttaat attttacaag
     9241 atttacgtgg tggccgctat gatgtcttgg ttggtgttaa tcttctgcgc gaaggtttag
     9301 atttgcccga agtatcttta attattattc ttgatgccga tatgtccggc tttttgcgca
     9361 atgaaacatc attaattcaa actattggcc gggcggctcg acatattaac ggacaagtta
     9421 ttatgtatgc cgaccaaata actccagcta tggattatgc cattaaggaa acgcaacgac
     9481 gtcgacaaat acaggccgat tacaatcagc aacacggcat cactccgact agtattacta
     9541 acgctaattg gtcaaattta gactaagcaa tctattgagc caaataacaa atccgccgca
     9601 gcccgctgtc ttcaactaac gcataaatag ttggccctga cgaatcttga ctatcaggat
     9661 taatcgttaa taggctgcta gtggcggaaa actgccaacc atatttggtc tgcaaatcat
     9721 catcaagacc gagagcaatt atccgaccat ttttaatatt aatgatggcc gccttgtgac
     9781 aatcgtggcc acaagaccaa ctagctaaat gaaaatgacc ggccagattg acagcttgac
     9841 gttggtatga ttgattaata gcttgtcttt cggaatcgct taattgatag ccagaccaat
     9901 caatagcggc cgggctatcg gtaacaatag cagtccgcca atcagccgcc gataatggct
     9961 tgggacaaac aatcttagca atatcttgct tagtcaccgt cgctgctggc ggtaaatcta
    10021 ctggcgatgg aaagtctggc gccgctaccg gcattggact ggattgattg tcggtatcag
    10081 tcgtcgactg ataacggcgc gcaaccttga taccagccaa aacattagcc aatacgaaag
    10141 cggttaataa aatccagatt aaacgcttat tcatattctt taattattac cctccttacg
    10201 aaaactaaca ctaataaccg gctcatattt ttcttgatac tcatcaataa caaccacggg
    10261 ctgaccagcg gttaactgat taataactgc gtcagccaga agagccttgt cattggccat
    10321 ctcgccctta agatcgtcca aacgatctag ctcactacgt aaatcgctgc tgatagcttg
    10381 aataaccatc tggcgtttgt cgcgaataat ttttaattcc tcggttaatt gttgatattc
    10441 tgaagaatta gctaaagcat cacgataaac ggcgtttaaa gtcttgcgtt cattttttaa
    10501 tttctgtaat tggttaaata tttcttgggc ggttttcata tcttgacaaa aatactaaat
    10561 tgtgatatat tagcaggtta ttataaccac ttttaagtat ttaaaaagca ctcaatggct
    10621 aaaatcattc gctacagccg tataactctt gcctatatct gcctattgct gggggtggct
    10681 ggtttggttt tgccaatctt acccgggtgg atttttatgc tagttggctt agaattgctg
    10741 attcgttatc agccaaccaa taactgggcg ccaagaatta aaaatcgtta ccaaggctgg
    10801 gcgaataaat ggcaacaatg gcggtcacca actaattata acaataatta actaattcac
    10861 caagaacaaa ttattgctgc tgtttaattt tctcttgcag caattgtaaa aagccatcct
    10921 gatcaatatt tagcaattct gtctgacctc gttggcgaac cgctaactga cccgaggcaa
    10981 tttctttatc gccaacaacc aagacataag gaattttttc cgtagcggcc ttgcgaattt
    11041 tattacccac tgtttcatcg ctctcatcaa cctctactcg gatatcgtta tatcgcaact
    11101 tatcagctaa ttgttgacaa taatcatggt gctgatcatt aacagtcaaa atcttaactt
    11161 gaactggcga taaccacaac gggaaaacgc cggcataatt ttcaattaat aaagcaataa
    11221 accgttcaaa ggaaccaaac agcgctcgat gtaagacata aggctgttcg gcttgacctt
    11281 gagagttagt ataattcatg gtaaagcgct cgggcagatt aaaatcaaac tgcaaagtgg
    11341 aacattgcca ttcgcgaccc aaagcatctt taagtttgaa gtccaatttt ggcccataaa
    11401 aagccgcctc ccctttttct tctttaaaat taagcttctt ctcaatggcg actttcttta
    11461 aaacctgttc ggtaaattcc caaccctcat cagagccagc atatttggat ttgttttctg
    11521 gatcgcgtaa tgataagtaa acactgatta aatcggtgtc aaaaccgaat gatttaaaaa
    11581 taaatagaat aaagtcaact acccgcttca gctcattttc cacttgatcg gcccgacaga
    11641 taatatgggc atcatcttgg gtaaaaccgc gcactcgagt caagccggat aattccccct
    11701 ttttttcata acgataaacc gtgccgcatt ccgcataacg cagcggcaat tcacggtaag
    11761 accgtggttt attctgataa atttcaatat gaaatgggca attcatcggc ttaagcaagt
    11821 attcttctga ctctttgact ttaacccccc gttggcgttc ctctagagtt tggccggcct
    11881 ccaatggtgg atacatgctg gcattataaa atccccaatg accagaagtt tcccataagg
    11941 tcttattgcc gatatgcggg ctgcgtacta gactgtaacc gttgcggaga tgctggtttg
    12001 accaaaaatc ctcaattact ttccagagaa ttgctccttt gggatgccat aacggcaagc
    12061 ctaagccaac ttgttcatca atatgaaaaa gatccagctc acgcccaagc ttgcgatgat
    12121 cacgctgttc agcttcggcc aataattgca aataattatc cagctcaacc tgattattaa
    12181 aagctaaagc ataaactcta gtcaacatct tatttttttc actgccgcgc caataagcgc
    12241 cggccaaatg atgcaccaaa aaaccagctt gacgcaaatc agctgtggag gcaacatgcg
    12301 gtccgcgaca taaatcagca aattgaccga cggtataata actaacctcg gtttcgccct
    12361 gggcctgtag gtcagcaata atttccgctt tataagtctg accagcggct tgctcggccg
    12421 ctaaagcgtc aacggctgac ttaatttctt gattaaatgg caaatcagca gcaattaatt
    12481 ctaacatctt ttgatttaac agcggcaact gatcttcgct taatttatca ggaccaaaat
    12541 caaaatcata ataaaaacca ttatcaatag ctggaccaat agccggccgg acattaggat
    12601 agagctcggt aacggcagcg gccaaaatat gagccaaaga atggcggata acttctaaag
    12661 gataatcagg catattagtt aataaaaaaa tctcattcca acaataaaca aaagagttta
    12721 tcgctagaac gagacttttt aggactcgcg gttccatcta gcttcccgtt ggcgggcgct
    12781 taattaaaga tggcgactta gggccgaagg ccgatattct cccgcggttg gttggcgcgg
    12841 caatcatcgc cagatagata cttctatagt ataaacttta accaaaaagg tcaagcactc
    12901 caaacccttg acattattaa aaaaaatgct aatctataaa aacaaggtct attaattata
    12961 ggcctggggg ataagccgaa ggcaaacgaa tgtctctaag ttgagacaaa ttcggactct
    13021 gtatttatga gtcgcttctt atcccccaac aattttgttc cttcgaacta cccccctacc
    13081 cctcctaaag tggtcctaag ccgatcctag tgatcggctt ttttattttt aaacatagta
    13141 atgtttagct ttaaattact ggttaaacta gtctaatata aacaacaaga acaaatgatt
    13201 attttattat ctctaagttt tgtggtaaaa taaaaataat caataattat aaacttatgt
    13261 cttcacgatt aattatcgct agtttattat ttatagcg
//
LOCUS       DAOPAI010000017        13216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_4682_length_13216_cov_5.822734, whole genome shotgun sequence.
ACCESSION   DAOPAI010000017 DAOPAI010000000
VERSION     DAOPAI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4682_length_13216_cov_5.822734"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..2010)
                     /locus_tag="PKV49_02605"
     CDS             complement(100..2010)
                     /locus_tag="PKV49_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPL22532.1"
                     /translation="MPIPKLKIKKITGHKIIWPVISLIIVGLIITSGYLVIDHFQERF
                     YPNTIAAGQDISGLTKQQANDLLLARWNNLVEQGWTFQIPTKTVTIYPTASLTEGAPP
                     LLSFDNIATINQAWENNRADVWWQKLNKIITGWLFGRRTKAVIAINEKLLIQELQANF
                     QSLITASQPAKITVHQDGTYQITPEITGQIIDYPSGLKEFGQQLLELQQQPITLNIND
                     DQPLIQQSLIGNLQPAIEQYLSLAPIKLTASSSDWSINQAVLADWLALEIQAGQITVG
                     PASSSISQYLTTKLATEIDHEPQAGQFQRSGGRLTQFRPGKDGQKLNVASSSEAISQA
                     IRYGTSTVQLIIDEIKNPLNDTDPASLGIVDLLGTGTSTFTGSSANRIHNIKIGTNQI
                     SGQLIAPGTEFSVVKTLGDINAAAGYKQELVIKQNKTVAEYGGGLCQVGTTVFRAALA
                     SGLPITQRQNHSYRVSYYEPAGTDAAVYDPWPDLRFINDTNNYLLILGEIAGNQLSFS
                     IWGTKDGRVVQQTKPIIYNIVKPPAKQIIETDTLPAGQIKCTERAHNGADTYFDYTVT
                     YANGETKSKRFSSHYVPWREVCLVGKTTASTTTATTTPPTTTTPAITTTPAVSSTQPI
                     ATSTQSQQQDEL"
     gene            complement(2012..2227)
                     /locus_tag="PKV49_02610"
     CDS             complement(2012..2227)
                     /locus_tag="PKV49_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22533.1"
                     /translation="MHCQLTLARLNQDQAILTDKSGRNFIFPTDQLPAGSQPGTILTC
                     LLMNPSQPDSADQQLAKDILNELLKID"
     gene            complement(2233..2649)
                     /locus_tag="PKV49_02615"
     CDS             complement(2233..2649)
                     /locus_tag="PKV49_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPL22534.1"
                     /translation="MTKTTSTANQSARRTTQSNEAADWLTISGHEGQLSLDIYQTEKN
                     LIVKSTIAGARPEEIQISLKNDLLTIRGKRDLPEDIAEADFLFRECYWGPFSRSIILP
                     VDVQADKIKATMDNGVLTIVLPKAPQAKQIVIKVKS"
     gene            complement(2659..5184)
                     /locus_tag="PKV49_02620"
     CDS             complement(2659..5184)
                     /locus_tag="PKV49_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPL22535.1"
                     /translation="MAKRKKRNLNANQPGLEYLSLGGRSLSAESKKSISAIVLALLAA
                     ISLLSLIKPEGLIGQYLYNAISQLLGWGRWFLPIFLLALAVFIYYRERLRITTIKFVG
                     WGLLFAGLQSFFHFCLAENHWHSYIADGQGGGYVGYGLTWGLTSLFGYWSSLVLTIAI
                     LIISLIFIFNTNLSCLLGRRSIFNKVLIEPLISGWHYIINLFRRADNKNLSPEEELAT
                     VYTSEPELNELLDNHQPETAKIKPLPTTAKTTVPADNETKKDKPSKTTSKIDLPIDLL
                     DRQLDKPESGDTVISAQRIKTTLENFDIPVDVAEITVGPTITQYAIKPADGIRLEKIT
                     GLAGNLSMALASPHPIRIEAPIPGKSLVGIEVPNQTIATVGLRDMLDSPNFRQRKNNT
                     MIALGKDVTGKVWMEDLASMPHLLVAGTTGSGKSVMLNCIILSLIYENSPEDLRLIMV
                     DPKRVELSIYNGIPHLLTPVINEASKTINALGWALNEMSRRLEMFQKNHAKDIKSFNQ
                     QFPNQKIPYIIFIIDELADLMMMSRKEVEASIIRLAQMSRAAGIHLVLATQRPSVDVI
                     TGVIKGNMPARIAFTVASQVDSRTILDTAGAERLMGKGDMLFSSPRIAKPVRIQGAFV
                     SENEIKRVVNYIKARSGEPQYLKDVIEKHSVPDLPNGGTLTTTTINGDDDLLEKAKAA
                     VINSGRASATYLQRMFKIGYPRAASLLDQLEEQGVIGPVNGAKAREIYISPEEYQAQK
                     NQPVSGQPLHNPATSSMPDNFLVNEIWSDHEPNENDSADDAIQTDTADDQLPLAATEK
                     ILADIYSEEPTNQNDDDEEDNQNQSEQALEFDDDEDQEKYFSQ"
     gene            5317..6318
                     /gene="rlmN"
                     /locus_tag="PKV49_02625"
     CDS             5317..6318
                     /gene="rlmN"
                     /locus_tag="PKV49_02625"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPL22536.1"
                     /translation="MNWLAWSQFLAGRPAFRQRQLEQAVFQQLVTDWSAVTTLSAADR
                     QWLTDNCPLDIKARIFGQDNDQAQKAQIIFSDGQRVETVLMRHGDGRRAVCLSSQVGC
                     PLGCQFCSTGQQGFQRNLSSGEIVEQALLWQRLLAVTGQRIDNLVFMGMGEPLLNLAA
                     VLTATEWLHRSNGFNIGWRHIAISTVGLVPELKQLTKYGQQIKLAWSLHAANDQLRRR
                     LMPINRRYALAAVKQAFLNYAQVANRRVMVEYLLLDQVNDSLVQAEELADFLADFPKN
                     LLVINLLTYNPGAGQFRPSQAKVRRHFANILQRHGYKVTERNSLGQAVIGACGQLAG"
     gene            6368..7807
                     /gene="metG"
                     /locus_tag="PKV49_02630"
     CDS             6368..7807
                     /gene="metG"
                     /locus_tag="PKV49_02630"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPL22537.1"
                     /translation="MAQFYLTTTLPYINSEPHLGFALEIVRADALARAHRLAGDNVFF
                     NTGVDEHGLKVWRKAQGLGLETQAYCDKLAASFNDLKKLLDLSYDNFIRTTDQHHQAA
                     AQEFWRRCLASGDIYKQHYSVKYCVGCEMEKTDSELENGHCPLHPDQDLELIDEENYF
                     FRLSKYQNQLLKLYDSQPDFVVPANRLKEIRNFVANGLQDFSISRLVSKLPWGVAVPD
                     DPEQVMYVWFDALVNYISAIGWPDKPEKFNQWWPAVQIAGKDNLRPQALMWQAMLMSA
                     GLPASRQIFIDGFITAGGQKMSKSLGNVISPVELVEKFGADVVRYYLLAEINDRDDGD
                     YTDERFMEVYQADLANGLGNLFSRLGNLAWKNGLNPLAPKIGQLYTKVIQSVENWQFN
                     QAVGYIWQVYRQIDKKLTEQQPWKLETAAAQKSLQPLVEDLVDAAYCLQIFLPTTAKT
                     ILNFFSQPITGKPKPLFPRFDQFNNNFKK"
     gene            7816..8331
                     /locus_tag="PKV49_02635"
     CDS             7816..8331
                     /locus_tag="PKV49_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPL22538.1"
                     /translation="MLAVDIFFIVVLGLAFLAGLRRGLFKVVGSLIGLVVGAWIASHY
                     YLVVFDWLVQHFSESWLINKIAIFIILFFVASNLTAWAFSFLGGVWEAITIIPLMKTA
                     NRLLGGVLNLAEAALSWSLILFFLSRYLPTSTELGQQLSQSIIAPYLMTIGKILWPLL
                     PVVLKQMQALW"
     gene            complement(8471..8707)
                     /locus_tag="PKV49_02640"
     CDS             complement(8471..8707)
                     /locus_tag="PKV49_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22539.1"
                     /translation="MPIVSISQGLSSDDLHVTALGCKTVEEADIILNHPQCAETTRQM
                     VVAKIKLFDKKEFNQVDENYHSDIVAVAKQKNGG"
     gene            8885..9736
                     /locus_tag="PKV49_02645"
     CDS             8885..9736
                     /locus_tag="PKV49_02645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPL22540.1"
                     /translation="MRLVDTHAHVNFNAFKDDGDEVIKRSLEVGVGMILVGSEARTSK
                     RAIDYANRYEHGVWAAVGLHPTHLFAARVKGDDYSFVTRGEEFDSEVYGKLADFSKVV
                     AIGEVGLDYYHLDEFDVTPDEIKDKQKQVLSQQLILAAKRELPVILHCRDAHNDLLPL
                     LQDFRRQYRQLLPVSRPWGVVHCFSGNESLANEYFAIGLLISFNGLITFSHDWDYLIK
                     KLPADKFLVETDCPYLTPAPFRGQRNEPGLVSLTAERIGQLKGWSLERIADITTDNAR
                     NLFNIDW"
     gene            9741..10811
                     /locus_tag="PKV49_02650"
     CDS             9741..10811
                     /locus_tag="PKV49_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPL22541.1"
                     /translation="MEIILAIAVVLLIGVLVWLIVTKRDSGGRLIALQEKMGLLVTQN
                     SELKNLLDEKLRETHQASQNQYLNTMNAVQSIANQSNRLLSDVTRNLTELQSSNKQIL
                     GFSSQLQSLQDILKNPKQRGVLGEYFLEEILKNVLPPGTFQVQYPFKNGEIVDAVVFV
                     RDKLIPIDSKFSLENYEKILNTKDDEQRINLEKSFKQDLKNRIDETSKYVRPAEGTMD
                     FAFMFIPSEAIYYDLLVNKVGAIQIETKDLIEYAFRDKHVIIVSPTSFLAYLQTVLQG
                     LKALQIEENAKTIKVNVEKLARHLASYEEFLRKLGNGLGTVVNHYNHAYQEFKKVDRD
                     IVKITGAEAQVAPLEIDRPQND"
     gene            10815..13139
                     /locus_tag="PKV49_02655"
     CDS             10815..13139
                     /locus_tag="PKV49_02655"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPL22542.1"
                     /translation="MVSRFVERRQQADWNIEAAILQTVVYFSLFKYPLSQWEVWSFLP
                     YKADLLTVRQSLAQLVAVGQLAEKNGYYYLPSQEAVIECRQQRYPVSVYKFKKAQRIA
                     NWLRWLPGVQFVALANLMGAYNLRASGDIDLFIITRPGCLWVCRFWAVLGLQLCGLRP
                     TPENRRDKICLSFWLSNNNLRIDRFKLPDEAGCPDWYYIYWLANLRPLVEQGISYTRL
                     IKQNPWLKTYLPNWRPGSYSPTINRQFVSRAAVVVSRLTAWLAAPWEKTMMYWQQRFL
                     PLAVKDLMNKDQRVVVDTTVIKLHTIDRRHHFRERLLVALKEIKTGFIEKIKADPSDY
                     RPSHSWLIKISQLLCLALCIVIPWSTRWIIKAGNLGGVFWEYGTISLYASDILLVMAL
                     LATYLAKPQKQLRLSRLDKYFIYWLIGLWLVSGISLLINGVQLELAVFSLLKLMIWSG
                     GWWCLLAWSDWPLRRWLSCILIGLTVPAILGISQFITQQAVANKWLGLAAHLPGDLGV
                     AVIEATNSWGQVTGRWLRAYGSFDQPNILGAVMAMAVLLAMIGWRRLSSNLARGFLTA
                     GIGLWSLAMLVSWSRAAWLAVFIGWLTAILLTTRQSKVNRKFLVIFLISLTVWLGLWL
                     WPFHDLGHSRLTGQTRLEQKSLIERQDGYRQAWLVLKNHPLGVGLGNYTGFLSQQSAV
                     VGPAWLYQPVHNAFVLAVSEIGWLAGGWLLVGLLVLIVAVGKHRLIVWPLATAIIWLM
                     SLDHWWWSLHSGLIWLWLLVAILLKIGRQPRNLL"
BASE COUNT         3397 a         2463 c         3152 g         4204 t
ORIGIN      
        1 ctgtaagctg tacctggttg agactgggtc tgtcctagag aaaagtttct tgcctgccct
       61 accggagtta atttgtataa acaacaacca ggcgagctct caaagctcgt cttgttgttg
      121 tgattgcgtg ctagtggcta tcggctgggt tgagctaacg gctggtgtag tggtaatggc
      181 cggtgtggtg gtcgtcggcg gtgttgttgt cgcagtagtc gtgctggcgg tagttttgcc
      241 aactaaacag acttctctcc agggaacata gtgagaactg aagcgtttgc ttttagtttc
      301 gccgttagca taagtaacgg tatagtcaaa ataggtatca gcgccgttgt gggctctctc
      361 agtgcatttt atttggccag ccggcagagt gtcagtttca attatctgtt tggccggtgg
      421 cttaacgata ttataaatta ttggtttagt ctgttggacc acgcgtccat ctttggtgcc
      481 ccaaatgctg aatgataatt gattgccggc gatttcacca agaattaaaa gataattatt
      541 ggtatcatta ataaagcgca gatccggcca agggtcataa acagcggcat cggtgccggc
      601 cggctcgtaa taactgacac gatatgaatg attttgtcgt tgagtgattg gtaggccact
      661 agccagagca gctcgaaaaa ccgtagtgcc gacctggcac aacccgccgc catattcggc
      721 cacggtttta ttttgtttaa taaccagttc ttgcttatag ccggcagcag cattaatatc
      781 gcccagagtt ttaaccaccg aaaattcagt ccctggagca attaattgac cgctaatctg
      841 attagtgccg attttaatat tatggatacg attggctgac gagccggtaa aagtagaggt
      901 gccagttccg agtagatcaa caatacctaa gctagccgga tcagtatcgt taagaggatt
      961 tttgatttca tcaataatta attggacagt tgaagtgcca taccgtatag cctggctaat
     1021 agcttctgaa ctagatgcga cattgagctt ttgtccgtct ttacctggcc gaaattgagt
     1081 taaccgcccg cctgatcttt gaaattgacc ggcttgtggc tcatgatcaa tttcggtggc
     1141 cagtttagtg gttaaatact ggctaatact actgctggcc ggaccgactg tgatttgacc
     1201 agcctgtatt tctaaagcta accagtcagc taagaccgcc tggttgattg accaatcact
     1261 actgctggcg gttagtttaa ttggggctag agaaagatat tgttcaattg ccggttgaag
     1321 gttgccgatt aatgattgtt ggattaacgg ttggtcgtca ttgatattta aagtgatggg
     1381 ttgttgctgc aattctaaga gctgttgccc gaattctttg agaccagatg gataatcaat
     1441 tatttgacca gttatttctg gagttatttg gtaggtgcca tcttgatgaa cggtaatttt
     1501 agctggctgg ctggcggtga ttagcgattg gaagttagct tgtaattctt ggattagcaa
     1561 tttttcattg atggcgataa cggctttagt ccggcggcca aatagccagc cggtgataat
     1621 tttatttaat ttttgccacc atacatcagc tctattattc tcccaggctt gattaatagt
     1681 agcaatattg tcaaatgaca ggagcggcgg cgcgccttca gtcaggctag ctgttggata
     1741 gatagtgact gttttggttg gaatttggaa agtccagcct tgttcaacca agttgttcca
     1801 gcgggctagt aataaatcat tggcttgttg tttggttaag ccagagatat cttgaccggc
     1861 cgctatagta tttggataaa accgttcttg aaaatggtcg ataactaagt atccagaggt
     1921 gataattaag ccaacaatga ttaaactaat tactggccaa attattttat ggccggtaat
     1981 ttttttaatt tttaatttgg ggataggcat gtcagtctat cttcagtaat tcattgagaa
     2041 tatctttagc cagttgttga tccgcgctgt cgggctggct ggggttcata agcaggcagg
     2101 ttaaaattgt gccaggctgg ctaccggcgg gcagttgatc ggttggaaaa ataaaatttc
     2161 ttccagattt atcggttaaa atcgcctggt cttggttaag gcgagctaat gttaattggc
     2221 aatgcatatt gattaagact taaccttgat gacgatttgt ttagcttgcg gggcttttgg
     2281 caagacgatg gttaaaacac cgttatccat tgtagctttg attttatcgg cttggacatc
     2341 aaccggtaaa ataattgaac gagagaatgg accccaataa cattctcgga ataaaaaatc
     2401 agcctcagca atatcttcag gcagatcgcg tttgccgcga atggttaaaa gatcattctt
     2461 gagagaaatt tggatttcct ccggtcgagc gccagcaatg gtagatttaa caattaaatt
     2521 tttttcagtt tgataaatat ctaaggacaa ttggccttca tggccactga ttgtcaacca
     2581 atcggcagct tcgttggatt gagtagtccg gcgggctgat tggttggcgg ttgaagttgt
     2641 tttggtcata ttaaaaaatt attgactaaa gtatttttct tgatcttcat cgtcatcaaa
     2701 ttccagcgct tgttctgatt gattttggtt gtcttcttca tcgtcatcat tttgattagt
     2761 cggttcttcg ctataaatat cggctagaat tttttcagtg gcagctaacg gcaattgatc
     2821 gtcggcagtg tcagtttgta tcgcatcatc agcagaatcg ttttcgttcg gttcatggtc
     2881 tgaccagatt tcattgacta aaaaattgtc cggcattgaa ctggtggcgg ggttatgcaa
     2941 tggttggccg cttaccggtt ggtttttttg cgcctgatat tcctcaggac taatataaat
     3001 ttctcgagcc ttagcgccat taaccggtcc gataactcct tgttcctcca gctgatccaa
     3061 taagctagcg gctcgtggat agccaatttt gaacattctt tgcagatagg tggctgaagc
     3121 tcggccgcta ttgatcaccg ctgctttagc tttttccaat aaatcatcat caccattgat
     3181 ggttgtcgta gtcaaggtgc cgccattggg taaatcagga acgctatgtt tctcaataac
     3241 atctttgaga tattgcggtt cgccactgcg agccttaata taattaacta ctcgtttaat
     3301 ttcgttttca ctgacaaagg cgccctgaat gcggactggt ttagcgatac gtggcgagct
     3361 aaaaagcata tcacccttac ccattaacct ttcagctccg gcggtatcta agattgtccg
     3421 tgaatcaact tgtgatgcga cggtaaaagc gatacgcgcc ggcatattgc ctttgatgac
     3481 gccggtaata acatcaacgc tcggccgctg agtggctaat accaaatgaa taccggcagc
     3541 gcgcgacatt tgcgctaaac gaataatgct agcctcaact tctttacgcg acatcatcat
     3601 caaatcagcc aactcgtcaa taataaagat gatataggga attttttggt tagggaattg
     3661 ctggttaaaa cttttaatat ccttagcgtg atttttttgg aacatttcca agcggcggct
     3721 catttcgtta agggcccagc ctagggcatt gatggtttta ctcgcttcat taattaccgg
     3781 tgttagcaga tgcgggatgc cgttataaat actgagctca actcttttgg gatcaaccat
     3841 tattaatcgt aaatcttctg gactgttctc gtagattagg cttagaatta tgcagttaag
     3901 cataactgat ttaccggaac cagtagtgcc ggccactaaa agatggggca ttgaagctag
     3961 gtcttccatc cacactttac cagtaacatc tttgcctaga gcaatcatgg tattattttt
     4021 gcgttggcgg aagttggggc tatcaagcat atcgcgtagc ccaacagtcg cgatagtctg
     4081 attgggcact tcaataccga ccaatgactt gccgggaatt ggcgcctcaa tacgaatcgg
     4141 gtgcggcgag gctaaggcca ttgatagatt accagccaga ccggtaattt tttctaacct
     4201 gatgccatcg gctggtttaa tagcgtattg agtaatggtc gggccgacag tgatttcggc
     4261 gacatcaact ggaatatcaa aattttccaa tgttgtttta atacgttggg cactaataac
     4321 ggtatcgccg ctttccggct tgtccagttg gcgatccaac agatcaatcg gcaaatcaat
     4381 cttgctagta gtcttgctgg gtttatcttt tttagtttcg ttatcagccg gaacggttgt
     4441 tttagcggtt gttggcagcg gcttaatttt ggctgtttcg ggttgatggt tatcgagcaa
     4501 ctcgtttaat tcaggttctg atgtatagac ggtagccagc tcttcttcgg gtgataaatt
     4561 tttattatca gcgcggcgaa agagattgat aatataatgc cagccgctta ttaatggttc
     4621 aattaggact ttattaaaga tactgcgtcg tcctaacaag caacttaagt tggtattaaa
     4681 aataaaaatt aggctgataa ttaaaatagc aatagttaat actaaactag accaatagcc
     4741 aaaaagacta gtcaatcccc aagttagacc atagccgaca taaccgccgc cttgaccgtc
     4801 ggcaatgtaa ctgtgccaat gattttcggc cagacaaaaa tgaaagaagc tttgtaggcc
     4861 ggcgaaaagc aacccccagc cgacaaattt aatggttgtg atgcgcaatc gttcccgata
     4921 ataaataaag acggccaaag ccagtagaaa tatcggcaga aaccagcgtc cccagccgag
     4981 cagttgacta atggcgttat acaaatattg gccgattagg ccctctggct taattaaact
     5041 tagtagactg atggctgcca gcagagctag cacaatggca ctgatgcttt ttttagactc
     5101 ggcactaagg ctgcgtccgc ccaaagataa atattccagt ccaggctggt tagcatttaa
     5161 attacgtttt ttccgtttag ccatatccta ttaataagca accgaaacgc tggaagcggc
     5221 ggttgaagct tgctttcttg atgttgaaag tataccatta ctgatgaatt tcaacaacta
     5281 ttattttatt attgatggtg ttaattttag tattttatga attggttagc ctggtcacaa
     5341 tttttagctg gccgtccagc ttttcgccag cggcaattag aacaagcggt ttttcagcaa
     5401 ctcgtaactg attggtcagc ggttacgact ttatcggcgg ctgatcgtca gtggctaact
     5461 gataattgtc cgctagatat taaggctcga atttttggtc aggataatga tcaagcccaa
     5521 aaagcacaga taatttttag cgatggccag cgagttgaaa ccgtgttaat gcgtcatggc
     5581 gatggtcggc gagccgtttg cctatcatca caggtcggct gtccgctagg ctgtcaattt
     5641 tgcagcactg gacaacaagg ttttcagcgc aatttatcat ctggcgaaat cgttgagcag
     5701 gcgctactgt ggcaaaggtt attggctgtg acgggtcagc ggattgataa tttggttttt
     5761 atgggcatgg gtgagccgct gcttaattta gccgcagtat taacggctac tgagtggttg
     5821 catcggtcca acggttttaa tatcggttgg cgacatatag cgatttccac ggtcggttta
     5881 gtgcccgaat tgaaacagct tactaagtat ggccaacaga taaaattagc ctggtcgctg
     5941 catgccgcta atgatcaatt acgccgtcgt ttaatgccga ttaatcgccg ctatgcttta
     6001 gcagctgtta aacaggcttt tttaaattat gctcaggtgg ccaatcggcg agttatggtt
     6061 gagtatttat tgttagatca agttaatgat agcctggtcc aggccgaaga gctagctgac
     6121 tttctggctg atttccccaa aaatttatta gtcattaatt tgttgactta taatcccgga
     6181 gctggtcagt ttaggcctag tcaggccaag gtgcgtcgcc attttgccaa tattttacag
     6241 cggcacggct ataaagtaac tgaacgcaac agcttgggtc aagcagtaat cggggcttgt
     6301 ggccaattgg ccggttagaa ttgcttttac tcctagccga tgataaaata agcttattag
     6361 atgatttatg gctcaatttt atttaaccac caccttgcct tatatcaact ccgagccgca
     6421 tctcggtttt gctttagaaa tagttagagc tgatgcgttg gctcgcgctc atcgtctggc
     6481 cggcgataat gtttttttta atactggcgt cgatgaacat ggtttaaagg tttggcgtaa
     6541 agcccaaggg ttgggcctgg aaactcaagc ttattgcgac aaattagccg ccagttttaa
     6601 cgatttaaaa aaattgcttg atttaagcta cgataatttt atccgcacca ctgatcagca
     6661 tcatcaggcg gcagcgcaag aattttggcg acgttgctta gctagcggcg atatttacaa
     6721 acaacattat agcgttaaat attgtgttgg ttgcgagatg gaaaagactg attccgaatt
     6781 agaaaacggt cattgtccgc tgcatcccga ccaggacctg gaattgattg atgaagagaa
     6841 ttattttttt cgtctatcca aatatcaaaa tcaattactt aagctttatg atagtcaacc
     6901 ggattttgtc gtgccggcta atcgtttaaa agaaattcgc aattttgtag ctaacggtct
     6961 gcaggatttt agtatttctc gtctagttag caaattaccc tggggggtcg ctgtaccgga
     7021 tgatccggaa caggtaatgt atgtttggtt tgatgcttta gttaattata tttcagctat
     7081 tggttggcca gataaaccgg aaaaatttaa tcaatggtgg ccggcggtgc agattgccgg
     7141 taaagataat ttgcggccgc aagcattaat gtggcaagcg atgttgatgt cagccggttt
     7201 gccagctagt cggcagattt ttattgatgg ctttattacg gctggcggtc aaaaaatgag
     7261 caaaagttta ggcaatgtca tttcgccggt ggagttagtg gagaaatttg gcgctgatgt
     7321 tgttcgctat tatttgttag cggagattaa tgaccgtgat gacggcgatt ataccgatga
     7381 acgatttatg gaagtttatc aggctgattt ggctaatggt ctaggtaatt tattttcccg
     7441 actaggcaat ttggcttgga aaaatggtct caatccgtta gcaccaaaga ttggccagct
     7501 gtatacaaaa gttattcagt cagttgaaaa ctggcagttt aatcaagcag tcggctacat
     7561 ctggcaagtt tatcggcaga ttgataaaaa attaaccgag cagcagcctt ggaaattgga
     7621 aacagccgcg gcgcaaaaga gtctgcaacc cttagtggaa gatttagtgg acgctgccta
     7681 ttgtttacaa atatttttac caactacagc taagacaatc cttaattttt ttagtcaacc
     7741 aataaccggc aagccgaagc cgttatttcc gcgttttgat cagtttaata ataattttaa
     7801 aaaataataa ataatatgct ggcagttgat attttcttta tcgtcgtttt gggcttagct
     7861 tttttagctg gtctgcggcg tggcttattt aaggtggttg gctcgcttat cggtttggta
     7921 gtcggcgctt ggatagctag ccactattat ttggttgttt ttgattggct agtacaacat
     7981 ttttctgaat cttggctaat taataaaatt gcgattttta ttattttatt ttttgtcgcc
     8041 agcaatttga cggcttgggc gttctctttt ttgggtgggg tttgggaggc tataacgatt
     8101 attccactaa tgaagacagc caatcggttg ctgggcggtg ttctaaattt agccgaggcc
     8161 gctttgtctt ggtcattgat tctatttttt ttaagccgtt atttaccaac tagcactgag
     8221 cttggccagc aattaagtca atcaataatt gctccctatt taatgactat cggcaagatt
     8281 ttgtggccgc ttttaccagt ggtgctgaag cagatgcaag cattgtggta aattataagg
     8341 ttattatttt agtgtttgct tatcttattg gttgttaagt tcctagacag ttaagattat
     8401 taaaaaaaga gggacataga gccggcgagc cggatatgtc ccgtaagaac ttgattttgt
     8461 gcggcggctt ttagccgccg tttttttgtt tagccacagc gacaatgtcg ctgtgatagt
     8521 tttcgtcgac ttggttgaac tcttttttgt caaagagttt gatcttggcg accaccatct
     8581 gtctggtggt ctcagcacat tgagggtggt tcaaaatgat atctgcttct tcaactgttt
     8641 tgcaccccag tgccgtgacg tgtaggtcat ccgaagacaa cccctgactt attgaaacga
     8701 tcggcatttt attgcctcct ttttattttt tgtcctattt agggacactt tgtacttatc
     8761 attaattctt tggcttgtca agcgcaggct tattttatgg gctaaacagg gtggacgaag
     8821 ccagtaaatt atgatagatt aaagctagtt gaggctttag ttttatttgg ataatttttt
     8881 gtttatgcgc ctagttgata ctcatgctca tgttaatttt aatgccttta aggacgatgg
     8941 tgatgaagtg attaagcgtt cattagaggt cggcgtcggc atgattttag tcggttcaga
     9001 ggcgcggact tccaagcgag cgattgatta tgctaatcgt tacgaacacg gagtctgggc
     9061 agcggttggt ctgcatccaa cccatttgtt tgctgcccgc gttaagggcg atgattatag
     9121 ttttgtaacg cgtggcgaag agtttgatag tgaagtttat ggcaagctag ccgacttcag
     9181 caaagtggtg gcaattggcg aagttggttt agattattat catttagatg aatttgatgt
     9241 cacgccagac gagattaagg acaaacagaa acaagtcctt agccagcaat taatcttggc
     9301 cgccaagcgt gaattaccgg ttattcttca ttgtcgcgat gctcataatg atttattgcc
     9361 attactacag gattttcgcc gccaataccg ccagctatta ccggtatctc gcccttgggg
     9421 agtggttcat tgtttttccg gtaacgagag cttggctaac gaatattttg ctattggctt
     9481 attaatatct tttaacggcc tcataacctt ttctcatgac tgggattatt taattaaaaa
     9541 attaccggcg gataagtttt tagtggaaac tgattgtccc tacttaacgc cggcaccatt
     9601 tcgcggccag cgcaatgagc ctggtttggt tagtttaacg gcggaacgga ttgggcaatt
     9661 aaaaggctgg tcgctggagc ggattgctga tataacgact gacaacgctc gtaatttatt
     9721 taatattgat tggtaattat atggaaataa ttttggctat cgccgttgtt ttgttaattg
     9781 gtgtattagt ctggctcata gtaactaagc gtgatagcgg tggtaggcta attgctttac
     9841 aggagaaaat gggtttattg gtgacacaaa acagtgagtt aaaaaatcta ttagatgaga
     9901 aattgcgtga aactcatcaa gccagtcaaa atcagtatct aaatactatg aacgcggtcc
     9961 aatctatcgc caatcagtct aatcggctgc ttagtgatgt aactcgcaat ttaactgagc
    10021 tgcaatccag caataaacag attcttggct tttccagcca gctgcagagt ttgcaggata
    10081 ttttaaaaaa tccgaagcag cgcggcgttc ttggtgaata ttttttagaa gaaattttaa
    10141 aaaatgtttt accacccggc acttttcaag ttcaatatcc ttttaagaat ggtgagattg
    10201 ttgatgctgt tgtttttgtt cgcgataagt taattccgat cgactctaaa ttttcattag
    10261 aaaattatga gaaaattctt aatactaaag acgatgagca gagaattaat ttggagaaat
    10321 cttttaaaca agacttaaaa aatcggattg atgaaacaag caaatatgtc cggccggccg
    10381 aaggaactat ggattttgcc tttatgttta ttccatccga ggcgatttat tatgatttat
    10441 tggttaataa ggttggggct attcaaattg aaaccaaaga tctgattgaa tatgcctttc
    10501 gggataagca tgttattatt gtttcgccaa cgagctttct agcctattta cagacggttt
    10561 tgcaaggttt aaaggctttg cagattgaag agaatgccaa gactattaaa gttaatgtgg
    10621 aaaaattggc tcggcattta gcctcgtatg aagaattttt gcgcaagcta ggaaatggac
    10681 tgggaaccgt ggttaatcat tataatcatg cttatcaaga gtttaagaaa gttgatcgcg
    10741 atattgttaa gattaccggt gccgaggctc aagtagcgcc attagaaata gatcggccac
    10801 aaaatgatta agttatggtt agtcgttttg tagagcgccg acaacaggct gattggaata
    10861 ttgaagcggc tattctgcaa acggtggtct atttttccct atttaaatac ccacttagtc
    10921 aatgggaggt ttggtcattt ttgccgtata aagctgattt actaacagtc cgtcaatcat
    10981 tggcgcagtt agtggctgtc ggtcagttag ctgaaaaaaa tggttattat tatttaccga
    11041 gccaggaagc ggtgattgaa tgtcgacaac agcgctatcc agtgtcagtt tataaattta
    11101 aaaaggcgca gcgtatagct aattggttac gctggttgcc gggtgttcag tttgtggctt
    11161 tagccaattt gatgggagcc tataatctcc gagccagcgg tgatattgat ttatttatta
    11221 tcactcgtcc gggttgtttg tgggtgtgtc gtttttgggc ggtgcttgga ttgcagctat
    11281 gcggtttgcg gccaacgccg gaaaatcgtc gcgataaaat ctgccttagt ttttggctgt
    11341 ctaacaataa cctgcgtatt gatcgtttca aattgcctga tgaggccggt tgtcccgatt
    11401 ggtattatat ttattggcta gctaatttac gaccgttggt tgaacaaggt attagttata
    11461 ctaggcttat taagcaaaat ccgtggctaa aaacttattt gcctaattgg cgtcccggta
    11521 gttattcgcc aacgattaat cggcaatttg ttagccgagc cgctgttgtt gtttctaggt
    11581 taactgcttg gctagctgct ccctgggaga aaacaatgat gtattggcaa caacgatttt
    11641 tgccattggc ggttaaagat ttaatgaata aagatcaacg agtggtcgtg gacaccacag
    11701 taatcaaact gcacactatt gatcgtcgtc atcattttcg cgaacgtctg ttggttgcct
    11761 tgaaagaaat aaaaaccgga tttatagaaa agatcaaggc tgatccgtcg gattatcgtc
    11821 ctagccatag ctggttaatt aaaattagcc aattattgtg cctggcgttg tgtatagtca
    11881 taccgtggtc aacgcgttgg attattaaag ccggtaattt aggcggtgtt ttttgggaat
    11941 acggcacaat tagtctttat gccagcgata tcctgctggt tatggcttta ttggctacct
    12001 acttagctaa accccaaaaa cagctgcgat taagtcggtt ggataaatat tttatttatt
    12061 ggcttattgg tttatggtta gttagcggta ttagcttatt aattaatggc gtgcagctgg
    12121 agttggctgt ttttagtctg cttaagttga tgatttggag cggtggctgg tggtgtttat
    12181 tggcttggtc agattggccg ttgcgtcgct ggctcagttg tattttaatt gggttgacgg
    12241 ttccggctat tttaggtatc agtcaattta ttactcagca agccgtagct aataaatggc
    12301 taggtttggc tgcccacttg cctggcgatt taggcgtggc cgttattgaa gcgactaatt
    12361 cctggggaca agtaaccggg cgctggctca gggcttatgg cagttttgat cagccaaata
    12421 ttttaggagc ggttatggcc atggctgttt tgttggcgat gattggttgg cgtcgtctca
    12481 gcagtaattt agctcgcggt tttttaaccg ccggcatcgg tctgtggtcg ctggcaatgc
    12541 ttgttagctg gagccgagcg gcctggctgg cggtatttat tggttggttg acggcgattt
    12601 tattgacaac gcgccaatcg aaggttaatc gtaaatttct ggttattttt ttgattagct
    12661 taacagtttg gctaggttta tggttgtggc catttcacga tttggggcat agccgtttaa
    12721 ctggccagac gcgcttagaa cagaaatctt taattgagcg tcaagatggc taccggcaag
    12781 cttggttagt tctaaaaaat caccccctgg gagtgggttt aggcaattat actggttttt
    12841 taagtcagca atcagcggtt gttggaccgg cctggcttta tcagccagtt cataatgctt
    12901 ttgttttagc agttagtgaa atcggctggc tggctggcgg ttggttgtta gttggcctgt
    12961 tagtgttaat agtcgcagtt ggaaagcaca gattaatagt ctggccatta gcaacggcga
    13021 ttatttggct aatgtcttta gatcattggt ggtggagttt acatagcgga ttgatttggc
    13081 tctggttgtt ggtggcaatt ttattaaaaa ttggtcgaca gccaagaaac ctgctataat
    13141 tattttgtat tagttgaata taattttaat ttattttatg aaaaaaagct tatatttatt
    13201 aggtttagtc gccgcc
//
LOCUS       DAOPAI010000018        12792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_4881_length_12792_cov_4.494308, whole genome shotgun sequence.
ACCESSION   DAOPAI010000018 DAOPAI010000000
VERSION     DAOPAI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4881_length_12792_cov_4.494308"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKV49_02660"
     CDS             complement(<1..916)
                     /locus_tag="PKV49_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HPL22543.1"
                     /translation="MLVDKIISLANFEQAYLEIVGSFISDHKNRRYHGLDGHFLCDHD
                     LTSQQLLISCQKELLTGQPISPAISIKIPKRNKPDSYREVFVYNIRERIKAQAIYRII
                     LPTLDNYFSERLFSYRPGRPPYLAAKQFCHNYRRNFSSSQALVIDLKNYSEQINQDIL
                     ISQLENFFNDKSLLNLLKLFIGNSICRQGVIGQLDQGLVQGVPLIALFANLYLTEIDL
                     KYKRLANFYVRVGDDIAFLDNNYQRLSSTKDQLFTDLTALELVFNKNKIYLGPAAGDF
                     NFLGYKLANGKISLEPALVERILANWRQI"
     gene            complement(1318..1710)
                     /locus_tag="PKV49_02665"
     CDS             complement(1318..1710)
                     /locus_tag="PKV49_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPL22544.1"
                     /translation="MSIIIASGPVIVRNRQVLLVKHGDDFWKFCGGKVDYINGEGLIS
                     AAQREAKEELGIDIKIINPEPFLFYVKKNNGDGLVDIILVHYLTDFIGDIIPGGDIEQ
                     WAWIDLGSLDKEDLAPNIIPTLKYFKII"
     gene            complement(1791..2036)
                     /locus_tag="PKV49_02670"
     CDS             complement(1791..2036)
                     /locus_tag="PKV49_02670"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPL22545.1"
                     /translation="MSTIKIIIAVALSYAGFSVIKDYGGQFDLFGRPFKFSKVNVADS
                     LATAAALCMGEGDESQPLAMISGAPIKYYNPVDRGVS"
     gene            complement(2051..2926)
                     /locus_tag="PKV49_02675"
     CDS             complement(2051..2926)
                     /locus_tag="PKV49_02675"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HPL22546.1"
                     /translation="MKRAIIGLILLSLTAAVILAIYLAGSGQLAVSHDNQLPADGLSN
                     DSPVILESPLAESIINSPVIISGQARGQWFFEASFPVKIIDAHNQVLGLASAQAQSDW
                     MTNDFIPFKATVDFSQPTTSTGFIVLFKDNPSGLPEHDAEVKIPVKFFTATDVTAESQ
                     RTVKFYYYNAAQDLDEQGNISCGQSGLVAVNRQLPLSISPIKDTIQAFLSSPLTEADR
                     AAGLASEFPLTGVSLVDLALRDGNLIIKLSDPYNKTSGGSCRVSILKQQLEAVARQFP
                     EVQAVDLQPAELFQP"
     gene            complement(2938..4332)
                     /gene="asnS"
                     /locus_tag="PKV49_02680"
     CDS             complement(2938..4332)
                     /gene="asnS"
                     /locus_tag="PKV49_02680"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPL22547.1"
                     /translation="MSLTAIKGLYRETATYINQEIKVGGWVRTVRVSKGVGFIEINDG
                     SFFKNLQVVFNNNLPNFLEVEKLSIGSSIEVRGRLIESPAAGQSFELSAQEVIIINQA
                     AADYPLQKKGHSFEFLREIAHLRSRGNTFSAIFRLRSVLSYAIHQFFQEQGFSYVHTP
                     IISGSDCEGAGEMFTVTTLDLTKPLAKNDHGQVDYSQDFFGKKVGLTVSGQLEAETLI
                     AGLSRVYTFGPTFRAENSNTTRHACEFWMIEPEMAFADLADDMDLAEKMIKYLINRVL
                     NELPEEMEFFNKFIDHGLLERLHKVLEADFIRLTYTQAIDLLLESGEKFNYPVAWGID
                     LQTEHERYITEKIFGQPVFITDYPKDIKAFYMRQNDDGRTVAAMDLLVPGIGEIIGGS
                     QREERLDLLEARIKELGMKLEDYWWYLDTRKYGSVNHAGFGLGFERAIMYLTGMANIR
                     DVIPFPRTPKNAEF"
     gene            4465..7173
                     /locus_tag="PKV49_02685"
     CDS             4465..7173
                     /locus_tag="PKV49_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22548.1"
                     /translation="MKNNLNKFLILVACLLVGACLHTANAAKDNGYRPKLGGRWLIQV
                     EELGQAYYVNPTDQLAYPLGKLSQTPCLIKTLALGINETDYYKFTQTRAPSRLSGRLL
                     LRAHAQGQAYYVDPISRRLYPINSVNDVSQLLKTAGLGINNNNLNKILAKNNKSLQIK
                     TCQQYHQSQQSNHNSANDNQQIPTNNQPLCFGARVYIHPLTQEICSQLTCNAITRSTL
                     KSQCLKGVCGAECSDNETRACTINGKIGKQGCNPSYCQFFTSCGLPVLSNGQTSNPTC
                     SGGRLEPNITISDDRSNGLAPNFCSNQTITFSTAITNCQQPSYQWLINDVVQDGHTDS
                     QYSTTVNENDIVKVVLNCADDCLTTATASAELLFNTAITSLTTANLQLTETDDDAVHL
                     SWDNTPCAEIYEVWRGYENNTSTMSLIATTSETSFDDQDLYLANDYYYAIKPLNNQLL
                     LASNWSDIVAYHRCQESGQLCLIQKYKQNFDDMGTGLTLPDQWTIATSNLVRTINNFI
                     NSSANISYLGGNHYDGFESASNGTYNFGAGDKDSASDRSVGGLSSNTGSKSVNIYYAL
                     TNTTGNRINRINVTYAAEKYRNGSNPAAFALKFYYSFNGVYWWPVPDLTAQFDGPDDD
                     NYGFTQAPGERQELSSNIDLIQELNNNETLYLAWNYSVASNTAAGNARALAIDDITVK
                     SYMEVGQEIGYQQNFDAIGTSSTAVLPSNWQTARDSASPRVINTNGTLENKTGQVGGN
                     NLSSGAASGVYNFGAGTSTTAVDRSIGGLSSSSNNKSINFYYTFTNNSTNPITGFDVS
                     YDVEKYRNGSNPAGFSIKLFYSYDFTNWNNAGLGTSLLADSDNIGFTQAPSTTTNIAG
                     RISLPTALSPGDPLYFAWNYSVTTGTSTSNAQALGIDNVRLKWFNQ"
     gene            complement(7176..8294)
                     /locus_tag="PKV49_02690"
     CDS             complement(7176..8294)
                     /locus_tag="PKV49_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HPL22549.1"
                     /translation="MFKFGKVALCLFLFIGLFFGRCSLAQAEQPAGQADTFQAEVIEI
                     INTDNKLREDGTFFEQQDLRLRALDGRLKGQELIYYGVSDIEVGNANVYQIGDKVFVD
                     SYVNDIGQTVYYVVDFVRSGPLIWLTVVFVLVVLLIGRSKGLRALLGLLISFFIIIKL
                     MLPRILAGGNPLVISLISGVIILTFIIYLSEGFRRKSHIAIFSVLLSLTVTAILSVIF
                     TTLTRLSGMSQEESNFLIGTTATAINFRGLLLAGFVIGTIGVLDDIIIGQIEAVEQIK
                     KLNPALSARQVFNSAYKVGNAHLGAIINTLFLTYASTSLPLLLLFIVNAGQISLIRVF
                     NMEIISIEIVRTLIGSIGVVLSMPIATGLASLFLQRSD"
     gene            complement(8296..8847)
                     /locus_tag="PKV49_02695"
     CDS             complement(8296..8847)
                     /locus_tag="PKV49_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPL22550.1"
                     /translation="MFKFLIKHRLLIVVISLALIVSAVLIYCFIINQSASSIIKLEPG
                     KQFYFAYGSNMSLDNMNGRCGTGAKVIGPALLADYELTFDKRGYANIIPQAGQQVWGV
                     AWQIDQHCLKALDRYEGYPRMYNRQAVQVALRQQTITAIVYIEPADQSGGRPSRSYMN
                     NRVIPGAKENGLPAEWVKKLEQY"
     gene            complement(8871..9446)
                     /locus_tag="PKV49_02700"
     CDS             complement(8871..9446)
                     /locus_tag="PKV49_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPL22551.1"
                     /translation="MSFVLMTVGKTHSGKTTFGRELAQQLGPCCLLDSDEVADFLKRT
                     YSDLYQADYEEGSNQPTRGYYLKLAVVAEIYKAALSTNLPIIFTTSNAKKQLRQEICR
                     LAKESGRSTIMVYFNIADEVLVERINNNQRLGGNQAEIQGFNHFLTNIQTHRFEVPIA
                     AEADHYFEINRGSNKQVLEQVLKVMRPPVND"
     gene            9586..10254
                     /locus_tag="PKV49_02705"
     CDS             9586..10254
                     /locus_tag="PKV49_02705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPL22552.1"
                     /translation="MKKIYCLLAATLLLAGCGLKNQTVSTEQQLSTEKTAKEIINDTN
                     ISTMNTENSINNQTDLVGQYSQAILKTNLGDIQISLYGADSPKTVNNFLNLAQQGFYN
                     GTKFHRVIKDFMIQGGDPNSKSDDWSTHGTGGPGYRFADEFNNHLLVRGSLAMANAGP
                     DTNGSQFFIVTASATPWLDGHHTNFGQVTAGMEVVDQIEAAEVNQNDHPLADIVINSI
                     ELIK"
     gene            complement(10274..11995)
                     /locus_tag="PKV49_02710"
     CDS             complement(10274..11995)
                     /locus_tag="PKV49_02710"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPL22553.1"
                     /translation="MMKKLVYAIIAWLLLQPSLVLARQSVNGWYIKDFKTNIQLNKDS
                     SATITEYITADCGQSRGKHGIYRIVPTKVGRDDQAVKTPVELISITDQNGQAYKYQTT
                     KDSINQTVTWKIGDPHKEVKGENHYQIKYRVSNVIRQQDNFDEFYWNILGAFWDMDID
                     NFSAWIIFPPEIKADSVVIDYYTGQVGQKNKDLANYRWLNNILVFESTKTIPKNNGIT
                     ISLSFAQNIFIPDESAELVGKTTIVEKIISKYLAFFAIANLLLLSFLALIAGLIIYLK
                     HGRNPKDKRPVIAEYGPPDNLDPLAVGTIWQDRLLVNKFMTALIISLAVKGVIKIKEE
                     IKDHIFYKLTDNPEAENSLSNSEKTLLNELFKDGATVVKKSELSSNFYTKVFNLHHQA
                     VNELKENGYFEKATFKWRNILLIAAIVLMAVSPIAGIILFVFYAITSHKKTPKGMDAL
                     LKIKGLKLYMETAEKHRQQFYEQENIFDQLLPYAIIFGLTKQWVKKMADIYGPEKIET
                     TVATWYVGGNFHLADINDLADSLNNSITQISQSIHNLSGSNWSSGGSYGSGSSGGGGG
                     GGGGGGW"
     gene            complement(12007..12477)
                     /locus_tag="PKV49_02715"
     CDS             complement(12007..12477)
                     /locus_tag="PKV49_02715"
                     /inference="COORDINATES: protein motif:HMM:NF039500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HPL22554.1"
                     /translation="MINLNELCCFLVKAKQSTYASGDESIKIKEEDGSTTLVFEDDNF
                     KYHDNYFGGEPYGGREVVFYQGAPVYMMVYYGFVAEEAGDINSVYSVLQKALSLPPKD
                     HPYRGPLELVDGDYRYSNEYRGEVNNFSGQERIEYNGKTIYEARYIGGLVDQVR"
     gene            complement(12502..>12792)
                     /locus_tag="PKV49_02720"
     CDS             complement(12502..>12792)
                     /locus_tag="PKV49_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPL22555.1"
                     /translation="GETIFETAKRETKEETNLDIDKFEIISVADELRYLQSDGKHYLN
                     IGVRGIYNGGEVIVMEPDKCEEWRWFDLNNLPDNLFEGTLLTINNFSKNKIY"
BASE COUNT         4119 a         2863 c         2175 g         3635 t
ORIGIN      
        1 aaatctgccg ccaattagct aatatcctct ccaccaaggc tggctctaaa ctaattttgc
       61 cattagccaa tttatagccc aagaaattaa aatctccagc ggctgggcca agatagattt
      121 tatttttatt aaagactagt tctaacgcag ttaagtcagt aaataattga tccttggttg
      181 aagataatct ttggtaatta ttatccaaga acgcaatatc atcgccaaca cgaacataaa
      241 aattagccag acgcttatat tttaaatcaa tctcggtaag atataaattg gcaaaaagcg
      301 caattagcgg aaccccctgg acaagtcctt gatctagttg tccaattact ccttggcggc
      361 atatagaatt accaataaat aattttagca aattaagaag cgacttatcg ttaaaaaaat
      421 tttccagctg actaataaga atatcttgat tgatctgctc agaataattt ttaagatcta
      481 taactaatgc ttggctagag gaaaaatttc gacgataatt atgacaaaac tgtttagccg
      541 ccaaatatgg tggacgaccg ggacgataag aaaatagacg ttcggaaaaa taattatcca
      601 gtgttggtaa aataattcga taaatggcct gcgctttaat ccgttcacga atattataaa
      661 caaaaacttc ccggtaacta tccggtttat tcctttttgg tatcttaatt gatatagctg
      721 gactaatcgg ctgaccggtc aataactctt tttggcagct tatcaataat tgttgactgg
      781 ttaaatcgtg atcgcacaaa aaatggccat caaggccatg atagcgccta tttttatgat
      841 cactgataaa actgccaaca atttctaaat aagcctgctc aaaattagct agactgatta
      901 ttttatctac caacatattc ttgagaatca cccccaggat aactaacgac tacacgctag
      961 ttataattta aattcaaatg atgcccgttc ctggggagca atccccaggg cgtggccttt
     1021 accacctacc gttaacttaa aactaaaccg atatcaacgg ctgtatattt agtctaatct
     1081 aacgattatt gatatcccca atagaggttg tgagggtgat tctaaagaat acttgtttta
     1141 gtataacaaa aaaatattat aaattccagc taaagacacg ctatctctaa ttggttaatc
     1201 tataatattt taatgatagc tacgccacct tctttagtgt cgctaatagt gagtctctat
     1261 tcaactaccc cgctactaaa tctatagcca tccttaatcc gcagcttaca aaaattttca
     1321 aataatttta aaatatttga gagtaggaat aatgttcggt gccaagtctt ctttatctaa
     1381 actgcccagg tcaatccagg cccattgctc aatatcccca ccaggtatta tatctccaat
     1441 aaaatcagtc aaatagtgga ccaagataat atctaccaac ccatcaccgt tatttttttt
     1501 tacgtaaaac aaaaatggct ctggattaat aatcttgatg tcaataccca gctcctcttt
     1561 agcttccctt tgtgccgcac tgattaaacc ttctccattg atataatcaa ccttgccacc
     1621 acaaaatttc caaaagtcat caccatgctt gactaataat acttgtctat tccggacaat
     1681 aaccggtccg gaggcgatta tgattgacat aaatagcaaa ttaacgattt attaaattat
     1741 taagcccaac cagtaatggt ttatatatat atccaactcg ctatctataa ttagctcacc
     1801 cccctatcga ctggattata atatttaatt ggcgcgccgc taatcatggc taacggctgg
     1861 ctctcatcgc cttcccccat acataaagcc gcggccgtag ccaaactgtc ggcaacatta
     1921 accttagaaa atttgaatgg tcgaccaaat aaatcaaatt gaccaccata atctttaatt
     1981 acagaaaatc cagcataact caaagccaca gcaataataa tcttgatggt gctcataatc
     2041 tttaattatc ctaaggctga aataattctg ccggctgtaa atcaacggct tgcacttctg
     2101 gaaattgccg agcaactgct tccagctgct gctttaaaat cgacactcgg caagaccccc
     2161 cggaggtctt attataagga tcggacaatt taataattaa attgccgtca cgcaacgcta
     2221 aatcaactaa agaaacgccg gttaacggaa attcactagc caggccagcc gcccggtcag
     2281 cttcagttaa tggactactt aaaaatgcct ggatggtatc tttaatcggc gagattgaca
     2341 gtggcagctg acgattaact gccaccaagc ctgactgacc acaactaata ttgccctgtt
     2401 catccaggtc ttgagcagca ttataataat aaaacttaac tgtccgctgc gattcagcag
     2461 ttacatcagt tgcagtaaaa aatttgaccg gtatcttaac ctccgcatca tgttcgggga
     2521 gtccagatgg attgtcctta aataaaacaa taaaaccggt actggttgtc ggttgagaaa
     2581 aatcaactgt cgccttaaac gggataaaat cattagtcat ccagtcgctc tgcgcttgag
     2641 ccgaagctaa acctaaaact tggttatggg catcaattat tttcaccgga aaagaggctt
     2701 caaaaaacca ctggccacga gcttggccgc taattatgac tggactattt attattgatt
     2761 ctgccaaggg actctctaaa ataaccgggc tatcgtttga taaaccgtcc gccggcaatt
     2821 gattgtcatg actaactgct aattgcccgg acccagccag atagatagcc aaaataaccg
     2881 ccgccgttaa tgacagcaat attagaccaa taatagcccg cttcatagga ttaactatta
     2941 aaactcagca ttcttaggcg tgcgtggaaa tggaataaca tcgcggatat tggccatgcc
     3001 agttaagtac ataatcgccc gctcaaagcc caaaccaaaa ccggcatggt tgactgaacc
     3061 atattttctg gtatccaaat accaccaata atcctccaat ttcatgccta gttctttaat
     3121 ccgcgcttct agcaaatcca accgctcctc tctttgactg ccgccgataa tctcgccaat
     3181 accaggcacc aataaatcca tggccgccac tgttcggcca tcatcatttt gccgcatata
     3241 gaaagccttg atgtctttgg gataatcagt aataaagacc ggttggccaa aaattttttc
     3301 ggtaatatag cgctcgtgct cagtctgcag atcaattccc caggccactg gataattaaa
     3361 tttttcgcca ctctccagta ataaatcaat agcctgagta taagttaaac ggataaaatc
     3421 agcctccaag actttatgca gccgctctaa taatccgtgg tcaataaatt tattaaaaaa
     3481 ttccatttct tctggtaatt cgttcagcac gcggttgatt aaatatttaa tcatcttctc
     3541 ggccaaatcc atatcatcag ccaaatcagc aaaggccatt tctggttcaa tcatccaaaa
     3601 ctcgcaagca tgcctggtcg tattagaatt ctcagctcga aaagtcgggc caaaagtata
     3661 gactcgacta agtccggcga ttaatgtttc cgcctctaat tggccgctaa cggtcaagcc
     3721 cactttttta ccaaagaagt cttgactgta atcaacttgg ccgtggtcgt tcttagctag
     3781 cggcttagtc aaatctaggg tggtcaccgt aaacatctcg ccagcccctt cgcaatcgct
     3841 accggaaata attggcgtat gaacataact aaaaccttgc tcttgaaaaa attgatggat
     3901 ggcatagctt aataccgaac gtaaacgaaa tatcgctgaa aaagtattgc cccgtgatct
     3961 cagatgggcg atttcccgca aaaattcaaa gctatgcccc ttcttttgga gaggataatc
     4021 agccgccgcc tgattaataa taatcacctc ctgagccgat aattcaaaag actgaccagc
     4081 cgccggtgat tcaatcaacc gacctctgac ttcaattgat gagccgatgc tgagcttttc
     4141 aacttctaaa aaattaggca aattattatt aaaaaccacc tgcagattct tgaaaaaact
     4201 gccatcatta atttcaataa aaccaactcc tttggacacc cttaccgttc tgacccagcc
     4261 gccaacctta atttcctggt tgatgtaagt cgctgtttca cgatataggc ccttaatcgc
     4321 cgttaatgac ataggctaac atttaatgta ataatatttt aactataccg taaataaatc
     4381 agaaaaacaa atgaccccct atgctttcag cgacaagtta ttgttaaaat aaaatatcct
     4441 gttaaaattt aataaaacac tgctatgaaa aataatttaa ataaattctt aattctagtc
     4501 gcttgtttat tagtgggagc ctgtttgcat acggccaatg cagctaaaga taatggctac
     4561 cgacccaaac taggtggtcg ctggttaata caggtcgaag aactggggca ggcctattat
     4621 gttaatccca ccgatcaatt agcctacccc ttggggaaac ttagccagac tccttgtcta
     4681 ataaaaacgt tggcactcgg tattaatgaa actgactatt ataaattcac gcaaactaga
     4741 gcgccgagcc gcctgtctgg tcgcttgctt ttgcgagccc acgctcaagg gcaggcttat
     4801 tatgtagacc caattagccg ccgcctttat cctattaata gcgtcaatga tgttagccaa
     4861 ctactaaaaa cagctggatt gggtattaac aataataatc ttaataaaat cttggctaaa
     4921 aataataaat cgctacaaat caaaacttgc cagcaatacc accaaagcca acaatccaat
     4981 cacaattcag ctaatgataa ccaacaaata ccgaccaata accagccgct ttgttttggc
     5041 gcacgtgttt atattcatcc tctaacccaa gaaatctgct cacaattaac ctgtaatgcc
     5101 ataaccagat caactctaaa aagccaatgt ttaaaaggcg tttgtggcgc cgaatgttcg
     5161 gataacgaaa ctagagcctg tactataaac ggaaaaatcg gcaaacaagg ttgtaacccg
     5221 tcatattgcc aattctttac ctcttgtgga ttaccagtac tatcgaacgg ccaaacgtca
     5281 aatccgacct gttccggtgg gagacttgaa ccgaatatta ctattagcga tgatcgctct
     5341 aatgggctag cgccgaattt ttgttctaac caaacaatta ccttttctac agctataact
     5401 aactgccaac aacccagcta tcaatggtta attaatgatg ttgtgcaaga tggacatact
     5461 gattcacaat attcaactac tgtcaatgaa aatgatattg ttaaagtagt tttaaattgc
     5521 gccgatgatt gtttaacaac agctacggct agcgctgaat tattatttaa tacagcaatt
     5581 accagcctaa ctaccgctaa tttacaatta accgagacag atgacgatgc ggtgcattta
     5641 tcctgggata atactccctg cgctgagatt tatgaagttt ggcgtggcta tgaaaataat
     5701 actagcacca tgtctctaat agccactact tcagagacta gttttgacga ccaagatctc
     5761 tatctagcta atgattatta ttacgccata aaaccactaa ataatcagct actattggct
     5821 agtaactggt ctgacattgt tgcctaccac cgttgtcaag aatctggcca attatgctta
     5881 atacaaaaat acaaacaaaa ttttgatgat atggggactg gattgacact gcccgaccaa
     5941 tggacaatag ccacaagcaa tctagtgcgc acgattaata atttcatcaa cagtagtgct
     6001 aatatcagtt atcttggcgg taaccattat gatggctttg aatcagcgag taatggaaca
     6061 tataattttg gggctggcga taaagactcc gccagtgatc ggtcagttgg aggactttct
     6121 tctaatactg gttctaaaag tgttaatatt tattatgctt taactaatac tactggcaac
     6181 cgtatcaacc ggattaacgt tacttacgcg gctgaaaaat ataggaacgg tagtaatccg
     6241 gccgctttcg ccttgaaatt ttattattca tttaacggag tttattggtg gccggtgcca
     6301 gacttaactg ctcaatttga cggacccgac gacgacaact atggctttac tcaggctcca
     6361 ggagaaagac aagaattatc tagcaatatt gatctaatcc aagaattaaa caataacgaa
     6421 actctatatc tagcctggaa ttacagtgtc gctagcaata ctgccgccgg taatgcccgt
     6481 gctctagcta ttgacgacat cacggtaaaa agttatatgg aagtcggaca agaaattggt
     6541 taccagcaaa attttgatgc tattggaacc agctccacgg ctgtcttacc tagtaattgg
     6601 caaacggctc gcgatagtgc ctccccaaga gtcattaata ctaacggtac tctagaaaac
     6661 aaaactggac aagttggcgg taataatctg tcaagcggag cagctagcgg cgtctataat
     6721 tttggagcgg gaacatcaac gaccgccgtt gatcgatcaa tcggcggact atcatctagt
     6781 agtaataata aaagtattaa cttctattat acttttacca ataacagcac taacccaata
     6841 accggctttg atgttagtta tgacgtagaa aaatatagga acggtagcaa tccggccggc
     6901 ttttctatta agctattcta ttcctatgat tttaccaatt ggaataatgc cgggttgggc
     6961 acttcattat tagccgactc ggacaatatc ggctttactc aagccccaag cacaacgaca
     7021 aatattgctg gcagaatttc attaccgacc gcactaagtc caggagatcc attatacttt
     7081 gcctggaact atagtgtcac cacgggaaca agcacttcta acgcacaagc tctgggtatt
     7141 gataatgttc gattaaagtg gttcaatcaa taatattaat ctgaccgctg caaaaatagg
     7201 ctggccaatc cggtagcaat cggcatagat aaaacaacac cgatactacc aataagagtc
     7261 cgaacgattt cgatactaat tatttccata ttaaacacac gaatcaaact aatttgacca
     7321 gcattaacaa taaataacaa tagaagcggc agcgaagtac tagcgtaagt caaaaatagt
     7381 gtattaataa ttgcccctaa atgagcattg ccaactttat aggccgaatt aaaaacttgc
     7441 cgagccgaca aagctgggtt aagtttttta atctgctcca cagcttcaat ctggccaata
     7501 ataatatcgt ctaaaacacc aatcgttcca ataacaaatc cggccaacaa aagaccacga
     7561 aaattaatcg ccgtcgcggt agtgccaatt aaaaaattgc tttcctcttg cgacatccca
     7621 ctaagccgag tcagagttgt aaaaataact gataaaattg ctgttaccgt tagagataat
     7681 aaaaccgaaa agatagcgat atgtgactta cgccgaaagc cctcggacaa ataaataatg
     7741 aaggttaaaa taattacgcc actaattaaa ctaataacca aaggattacc accggccaaa
     7801 atgcgtggca gcattaattt gataataata aaaaaactga tcaataatcc gagcaaagct
     7861 cgcaacccct tggaccgacc tattaataaa acgactaaaa caaaaacaac ggtcagccag
     7921 attaatggtc cgctgcgcac aaaatcaact acataataaa ctgtctgacc aatatcgtta
     7981 acataactgt caacgaaaac tttgtcacca atctggtaaa cattagcatt gccgacctca
     8041 atatcggaaa caccatagta aattagctct tgtcccttaa gccgaccatc caaagctcgc
     8101 aaacgcaaat cttgttgctc gaaaaatgtt ccgtcttcac ggagcttgtt atcggtatta
     8161 attatttcta tgacttcagc ctgaaaagtg tccgcttgac cggccggctg ctcggcctgg
     8221 gctaaactgc aacggccaaa gaaaagtcca ataaacaaaa ataaacaaag ggcgactttt
     8281 ccaaacttaa acatattaat attgttctaa tttcttaacc cactcagccg gtaaaccatt
     8341 ttctttagct ccaggaatta cccgattatt catatagctt cgcgaaggcc ggccaccgga
     8401 ttgatcagct ggctcaatat aaactatcgc cgtaattgtc tgttgcctaa gagcaacctg
     8461 aacagcctgc cggttataca tccgcggata accttcgtag cgatctaacg cttttaaaca
     8521 atgctgatcg atttgccaag caactcccca aacctgctga ccagcctgcg gaataatatt
     8581 ggcataaccg cgcttatcaa aagttaattc ataatcagct aaaagtgccg gaccaattac
     8641 cttagcgccg gttccgcagc gtccgttcat attatccaga ctcatattgg agccgtaggc
     8701 aaaataaaat tgcttgccgg gctccagctt tattatactg ctggcggatt gattaattat
     8761 aaaacaataa atcaacacgg cggagacaat caacgccaac gatataacaa caatcaacaa
     8821 cctatgtttt atcaagaact taaacattaa aaaaatattt aatacttaaa ttaatcattc
     8881 acaggtggcc tcatgacctt taatacttgt tctaaaactt gtttatttga ccccctgtta
     8941 atttcaaaat aatgatcagc ctcggcagct attggaactt caaaccgatg agtttgaata
     9001 ttggttaaaa aatgattaaa gccttgtatt tccgcctgat taccccctaa tctctggtta
     9061 ttattaatcc tctcaactaa aacctcatcg gctatattaa aataaaccat aatggttgat
     9121 cggccgctct ctttagctaa ccgacaaatt tcttggcgaa gctgtttctt ggcattagag
     9181 gtagtaaaga taattggtaa attggttgat agtgccgcct tatagatctc agccacgacg
     9241 gccaatttca aataataacc acgagtcggc tggttactgc cctcttcgta atcagcttga
     9301 tacaaatcag aataggttct ttttaagaaa tcagccactt catcagaatc taaaaggcaa
     9361 cacgggccca gctgctgagc cagttcgcgg ccaaaagtgg tcttgccgga atgagtcttg
     9421 ccgacagtca ttaaaacgaa agacatagta atctataaac aagctaactt acttaatttt
     9481 aattatatta tctcattaat ttgcagcaaa agcaaaaatt actatcttgc taaaccagct
     9541 tattttggct atattaaaat tatcaatctt taaatgactt tagctatgaa aaaaatttat
     9601 tgcctcctgg ctgccaccct attgttagct ggctgcggac tgaaaaatca aacagtttca
     9661 accgaacaac aattatcgac cgaaaaaaca gccaaagaaa ttattaatga cactaatatc
     9721 tccactatga acaccgaaaa ctccattaac aatcaaactg acttagttgg ccaatatagc
     9781 caagctatct taaaaactaa tttgggtgat atccagatta gtctttatgg cgctgactcg
     9841 cctaagacag ttaataattt tttgaatttg gcacagcaag gtttttataa cggcaccaaa
     9901 tttcatcgcg tcattaaaga ttttatgatt caaggcggcg atcccaatag caaatctgat
     9961 gattggtcca ctcacggtac aggcggacct ggctatcgtt ttgctgatga atttaacaat
    10021 catcttttgg ttcgcggtag cctggctatg gctaacgccg gtccggatac caatggttcc
    10081 caatttttta ttgttaccgc ttcggccact ccatggttgg atggccatca taccaatttt
    10141 ggccaagtaa ccgctggtat ggaggttgtc gatcaaattg aagcggctga ggttaaccaa
    10201 aacgatcatc cgttagcaga tattgtaatc aactcaattg agctaattaa ataaattgca
    10261 gaacgacggc tcgttaccaa ccgccaccac caccgccgcc accaccacca cccgatgaac
    10321 cgctgccgta actcccgccg ctgctccaat tggaaccgga taaattatga atcgattgag
    10381 aaatttgcgt aatagaatta ttaagactgt cggctaaatc attaatatca gccagatgaa
    10441 aattgccgcc gacataccag gtcgccactg tcgtctcaat tttttccggg ccataaatat
    10501 cggccatttt tttgacccac tgcttagtta aaccaaaaat aatagcatag ggcaacagct
    10561 ggtcaaaaat attttcctgc tcataaaatt gctggcgatg tttttccgcc gtctccatat
    10621 aaagctttaa acccttaatc tttaacagag catccatccc cttgggggtc tttttgtggg
    10681 aagtgatggc atagaaaaca aacaaaatta tccccgcaat tggactaaca gccattaaga
    10741 caatcgcggc tattagcaga atattccgcc acttaaaagt agctttctca aaataaccat
    10801 tctcttttaa ctcgttgact gcttggtggt gcaagttgaa aactttagta taaaaattac
    10861 tgctcaattc tgacttcttg acgaccgttg cgccgtcttt aaataattcg ttaagcaaag
    10921 ttttttctga attgcttaat gaattttctg cctcgggatt gtctgtcagt ttatagaaaa
    10981 tatgatcttt aatctcttct ttaatcttaa ttacgccctt aacggctaaa ctaataatca
    11041 acgcggtcat aaacttatta accaaaagac gatcttgcca aattgttccg accgctaaag
    11101 gatctaaatt atccggcgga ccatattcag caatcaccgg ccgcttatct ttagggttgc
    11161 gaccatgctt cagataaata attaaaccgg caatcagcgc taaaaatgac agtagtaata
    11221 aattagctat ggcaaaaaaa gccaaatatt tagaaattat tttttccact attgttgttt
    11281 ttccaactaa ttcagctgat tcatcgggaa taaaaatatt ttgcgcaaag ctcaaagaaa
    11341 tggtaatgcc gttattttta ggaatagttt tagttgattc aaaaaccaaa atattgttta
    11401 gccaacgata attagccaag tctttatttt tttggccaac ttgaccagta taataatcta
    11461 ttacaactga atcagcctta atctctggcg ggaagataat ccaagcgcta aaattatcaa
    11521 tgtccatatc ccaaaaagcg cccagaatat tccaataaaa ctcatcaaaa ttatcctgtt
    11581 gccgaataac attgctaaca cgatacttaa tctgataatg attttcaccc ttaacctctt
    11641 tgtgcggatc accaattttc caagtaaccg tctggttaat tgaatcttta gtggtttgat
    11701 acttataggc ttgaccgttt tgatcagtaa tgctaattaa ttcaaccggt gtcttgaccg
    11761 cttgatcgtc tcggccgacc ttggtcggaa caattcgata aataccatgc ttgccgcgag
    11821 attggccaca atcagctgta atatactcag tgatggtggc cgaagaatcc ttatttaatt
    11881 gaatattagt tttaaaatcc ttaatatacc acccattaac cgactggcgc gccaaaacta
    11941 aactgggctg caataataac caggctataa tcgcataaac taattttttc atcatagctg
    12001 gttttattat ctaacctgat caactaaacc accaatataa cgagcttcat aaatagtctt
    12061 tccattatat tctatgcgct cttggccaga aaaattatta acctcaccgc gatattcatt
    12121 gctgtaccga taatcaccat caactaattc cagcggcccg cgatatgggt gatccttggg
    12181 aggcaatgat aacgcttttt gtaaaacact ataaacacta ttgatatcgc cggcttcttc
    12241 agcgacaaaa ccataataaa ccatcatata gactggcgcg ccttgataaa aaaccacttc
    12301 ccgaccgcca taaggctcac cgccaaaata attatcatga tatttaaaat tgtcatcttc
    12361 aaaaactaaa gtggttgatc catcttcttc tttgatttta atagactcat caccgctggc
    12421 gtaagttgac tgcttggctt tcactaaaaa gcagcaaagc tcattgaggt taatcatata
    12481 attattattt aattaaacca attaatatat tttattttta gaaaaattat taatggtcaa
    12541 taatgtcccc tcaaataaat tatctggcaa attgtttaaa tcaaaccatc gccattcctc
    12601 gcatttatct ggctccatca caatcacttc tccgccgtta taaatacccc taacccctat
    12661 attcaaataa tgcttcccgt cactttgaag atatcttaac tcatcggcca ctgaaataat
    12721 ttcaaattta tcaatatcaa ggttagtttc ttcttttgtc tctcttttag cagtttcaaa
    12781 tatcgtttca cc
//
LOCUS       DAOPAI010000019        12240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_5134_length_12240_cov_5.235864, whole genome shotgun sequence.
ACCESSION   DAOPAI010000019 DAOPAI010000000
VERSION     DAOPAI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5134_length_12240_cov_5.235864"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PKV49_02725"
     CDS             complement(<1..1083)
                     /locus_tag="PKV49_02725"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPL22556.1"
                     /translation="MGWLYHITTIGCAMNISDSQRLASRLNELGWSATNQRSQADLAI
                     LVTCGVRQSAEDRIYGLIKRIKQDNPQTAVALTGCLSYRPDIQAKIGQQVDYWFTINQ
                     LTELEQIIPSPQPASSLTHLHDYLQIKPLYTSKISALVPIGNGCDNFCSYCVVPYARG
                     REKYRPADEIIAEVQNLLAADSQEITIIAQNVNSYRDPVSGLNFTGLLEQLDSLGNYW
                     LRFATSHPKDMSDELIAFMGRSRNLIHHLHLPVQSGSDRILQLMNRRYTSDHYRQLIA
                     AVRQVWPDVSLTTDIIVGFPGETDTDFQATAKLVTASGFDQVYIAQFSPRPGTAAAKL
                     PDDVPTATKKQRAIDLDQLVKAGTLSN"
     gene            complement(1085..2176)
                     /gene="dnaJ"
                     /locus_tag="PKV49_02730"
     CDS             complement(1085..2176)
                     /gene="dnaJ"
                     /locus_tag="PKV49_02730"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPL22557.1"
                     /translation="MATDYYQILGVSKTASAEEIKKAFRKLAHQYHPDKASGNEAKFK
                     EINEAYQVLSDQDKRAKYDRFGSAAFQGGAGGFNSANMGGFDFSQFYQGGQAGGFEAN
                     FGDLGEMFGDLFGFSRSSSRTDSGRGRDLEVGLKIDFLTAVFGGEEVINLEKQTTCPK
                     CQGSGAKTGSSPKNCPNCQGRGVIDQFQRTMLGNIRTQTTCPKCNGQGQIIDQPCPHC
                     HGQGRTVEKKPLTIKIPAGVANGQTIKFTGQGEAGRRGAKSGDLYVNVRVQPHPHLKR
                     NGHNIQSIVNLSFRQAALGDKIKIDTVDGPATLKIPAGTQSGQIIILRGKGVPKLNGY
                     GRGDHLVEAKVLTPTGLTKKQKEALELLD"
     gene            complement(2193..4100)
                     /gene="dnaK"
                     /locus_tag="PKV49_02735"
     CDS             complement(2193..4100)
                     /gene="dnaK"
                     /locus_tag="PKV49_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPL22558.1"
                     /translation="MSKIIGIDLGTTNCAVAIMEAGQPKILENAEGNRTTPSVVAISK
                     NGERLIGQTARRQAVTNPENTVYAVKRLIGRRFQDEEIQHDLKNVPYSIVQAGGGIKV
                     KLQDKEYTPQEISAMILAKLKADAEARLGETVTEAIITVPAYFDDSQRQATKDAGKIA
                     GLDVKRIINEPTAAALAYGFDKKKGQQVAVYDLGGGTFDISILDISEDTVEVKATNGD
                     THLGGEDFDQRIITWIIDEFKKDQGIDLSKDTLALQRIKEAAEKAKIELSTLNETEIN
                     QPFITTDANGPKHLVMKMTRAQLENLVGDLVAKTIEPCQKALADAGYQVGDIEEVIMV
                     GGMTRMPMVQAKVKEFFGKEPNLSVNPDEVVALGAAVQAGVLQGDVKDVLLLDVTPLT
                     LGIETLGGVATPLIERNTTIPTSKSQVFSTAADGQTSVEIHIVQGERPMATDNKSLGR
                     FILDGIPPAPRGVPQVEVSFDLDANGILNVKATDKASGKEQKITITASSGLSKDEIEK
                     LRQEAEKHAEEDKQKKEAAELKNQADSVIFQLEKLLKDSADKIKAEDKTELETKIAEL
                     KTAKDASDLEALKKKLEETNEVAQKIGAAMYQQTAAEANSAGANQADSATDSTAGTEA
                     NGPVEGEIEEE"
     gene            complement(4128..4562)
                     /locus_tag="PKV49_02740"
     CDS             complement(4128..4562)
                     /locus_tag="PKV49_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPL22559.1"
                     /translation="MRGLIKWNPLFDSLAEAEKVFNDFLPALRNDQHNFVPAIDMYED
                     KNNVIVETQLAGIQPENVQISIDNDILCIKGEGERHSEVDEKNYYRKEIRRGSFYRSI
                     QLPAHVLSSETKAVAEDGVLKIMIPKAPEAQPKTIKIESKKK"
     gene            complement(4619..5122)
                     /locus_tag="PKV49_02745"
     CDS             complement(4619..5122)
                     /locus_tag="PKV49_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPL22560.1"
                     /translation="MSTEPSKKNVKETKSKTKHGYSHEQYHELEDKYRRALAEQQNLI
                     KQHQKDRAHYVHYANEQLLLEILPVYDNLKLSCRHFNPDNTASWLEGINYIIKQFQNI
                     LSQQGVKEIPTIDQPFDHTTMDATEMVETADNQQDNLVAEEIKPGYQLKDRVITPARV
                     KVYRFKQ"
     gene            complement(5091..5777)
                     /locus_tag="PKV49_02750"
     CDS             complement(5091..5777)
                     /locus_tag="PKV49_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HPL22561.1"
                     /translation="METRPALILKLLTEDYIQTATPVASQALVDKYHLNFSPATVRND
                     LADLEEAGYIIQPHTSAGRIPTELAYRQLIATIEPKAPTSTVKKLLEPVLADFGHNWK
                     PLAKALAQLTNSGVFWSFHRYDSYYTGLSGLLGQPEFKQLSMIYDISQVIDSLEEIID
                     DLFNQIGEEPTIRLGNDSPFGAFSSTILTKYRFDQHIGLFGLLAPLRTDYQTNFNLIN
                     YVYRAIQEKC"
     gene            complement(5906..>6176)
                     /locus_tag="PKV49_02755"
     CDS             complement(5906..>6176)
                     /locus_tag="PKV49_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22562.1"
                     /translation="KLGAIELLIITGFFTNDLSAPTDILIISDHFNKTKLNDLIKKIE
                     QGTGRNIRYTLMDKAEFEYRQNMADIFLHQILNSQKEIIINKLGI"
     assembly_gap    6177..6186
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6187..6487)
                     /locus_tag="PKV49_02760"
     CDS             complement(<6187..6487)
                     /locus_tag="PKV49_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPL22563.1"
                     /translation="MLEKLFSSGARLKLLKLFLFSGPDDCFYIRQLARELDLQLNSVR
                     RELANLEEIGLLEVKTDHQQKLDKKFYQVNQNFYLLAELRALLAKAEIGQESKLRN"
     gene            6633..9041
                     /locus_tag="PKV49_02765"
     CDS             6633..9041
                     /locus_tag="PKV49_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22564.1"
                     /translation="MPYKLASLLLSSEQKSSSIAKIAWAEADQGDPANEVRFSRVGKI
                     FSLVEVYATNAKALLLLDFFSERPKLNYYQQPDLDKNEHPGLDDLEKFFTAAIQQTNH
                     DLAAFLAEHKISYLEPEVSATIGLLTERGLFLTSIGRNQAWMLLPGQDSGKQVVKMIV
                     PADDGAERTGRLFSEIFSGTLPNGNYCLISNEALAEYIEPATAVNLLTNKSPLVGAEK
                     LRHFLQQTNRRVDFLGLIIKRCHRDEDDEACLEMPAGLVSNNQSTTIATPLISNNLRL
                     TEERTEKILNPSGWSKFILLFKKIINSVWQWLIRLSRRDWVKVWRNNYHNGRAYYQET
                     KKKIRAWRGWPIIGRYSHRLLLIGRSAWYKIQPWFRQINNRWTAYWFKTAPWRCRLVE
                     KYWLPLERIIRPLINFFQAVSHRVRCFFNKGYQQPRTYWQRLSKRSRIFLGLAGVCLL
                     LLIIGMFWQYRRQIRQANWRQKTDQVALVEQKHNQIEANLLYNNLTSANQSLDEAAAL
                     LEQWQAAKEQLPADITERWQNALLKQEKLLGQVRHIYTLRDGQALANQPIAIVTSTDS
                     LLRLNGQLLAWTDSASRQVIYQNSRQEWQTVAWPEGWQIIVAAAADGQSWYFAAGDGQ
                     IASLSRDGQWKIWSLDKPISQIKALAFYGGRFYVVAGDNQIYRYQKQADNFTSGQQWN
                     TDQLPAGDIVGLVVDGQVYVFYSDGQAFKLNNGRSQNIAIKIAEPTTEGWSSCQLLAD
                     SDNFYCLESGYRRILLYGKDGAISGQYRLADRQAQAVAISSASKAIYLLTDQAIQKFV
                     LPTE"
     gene            complement(9105..9527)
                     /gene="rplL"
                     /locus_tag="PKV49_02770"
     CDS             complement(9105..9527)
                     /gene="rplL"
                     /locus_tag="PKV49_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPL22565.1"
                     /translation="MTEEMNKTEEQIEVPAKFQKLVDEIEKMSVIDLAELVKILEKKF
                     GVSAAAPAMMMAGPVAGAEAAPAEEKDSFDVELSDAGANKIAVIKAIREINQDLGLKE
                     AKDLVDAAPKMIKEGVKKEEAETIKAKLEEAGGKVTLK"
     gene            complement(9564..10094)
                     /gene="rplJ"
                     /locus_tag="PKV49_02775"
     CDS             complement(9564..10094)
                     /gene="rplJ"
                     /locus_tag="PKV49_02775"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPL22566.1"
                     /translation="MPKTKQQKEIILRDLDTKLAQSKAVVFASFNNLTVRDTEELRGQ
                     LLEEQGEFLAIKKTLLGRALNNIQLTEQPNVREFDGQVAIIFAYGDQVLPAKLLQKFQ
                     KTHEDKIAFLGGILDGQFLSAADVQSLANLPSKLELQARLVGALNAPVSGFVNVLAGN
                     LRGLVTVLQAVADQKA"
     misc_feature    complement(10103..10230)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(10301..11212)
                     /gene="mltG"
                     /locus_tag="PKV49_02780"
     CDS             complement(10301..11212)
                     /gene="mltG"
                     /locus_tag="PKV49_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPL22567.1"
                     /translation="MSTYYNNYPPQKKSTVYLVWLVSIIIFGGIFSGLYYITKKLENR
                     RARINYWQEQANQQADRLVTLIEGWNSQQIANKLADSQLNFSADDFLQISGYPKKNYQ
                     SLPVNKWPQDFSDRYEFLQDKPKYLGLEGYLFADTYRFHPSSSPEEVIIKLLNNFDRQ
                     LNTKMRQDIKRQGQTIYQIVTMASLLEKEVRSEKDMKIVADIFWRRIKNGQRLESCAT
                     LAYILGENKPQYSYEETRIDSPYNTYINDGLPLGPISSPSLKALNAAIYPTSNDYNFF
                     LTRPDNGETVFSRNFEEHKANKAKYLK"
     gene            complement(11213..>12240)
                     /locus_tag="PKV49_02785"
     CDS             complement(11213..>12240)
                     /locus_tag="PKV49_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPL22568.1"
                     /translation="NPKQFQALLGDKTMLRTTYERVAEILQQPDDLFISINELMVKRV
                     KVEVPEIKLNNIIVESESRNTGPAMCLEVCYLQHFCQPDDIIASLPSDDYISDNQAFA
                     DLLLVSEQFLQQNPDYILTPAVKPAYVDTGYSYLKAGQNLQSIGQETIFTVCNVVEKP
                     NEDYCRQLIDSGIYYCHTGMYVWRLQTIADLFAKLQPTMYQICQEVVGLLINYGDNEK
                     VRRLYGQLDKISIESAITNKADKLAMSVSNRVGWSDLGKWPVLKNILATEVSDNVTKG
                     RVITKKSNNNLVYCNLTDKLIVINDVNDLAIIDTGDVLFISSLKNSINTKEIIDDLKK
                     ESLDKYL"
BASE COUNT         3260 a         2500 c         2653 g         3817 t
ORIGIN      
        1 gttgcttaaa gttccggctt taacaagctg atccaagtct attgctcgtt gttttttggt
       61 ggcggtcggc acatcgtccg gcagcttggc agcggcggtg cccggtcgcg ggctgaattg
      121 agcgatatag acttgatcaa aaccgctagc cgtgactaat ttggcggtcg cttgaaaatc
      181 agtatcagtt tcaccgggga agccgacaat aatatcagtt gtcaaactga catcaggcca
      241 gacttgacgg acggcggcta ttaattggcg gtaatgatca ctagtatagc ggcgattcat
      301 taattgtaaa atacggtcgc tacctgattg aaccggtaaa tgcagatgat gaattagatt
      361 gcggctacgg cccataaaag cgatcagttc atcgctcata tctttagggt ggctggtggc
      421 gaaacgcagc caatagttac cgaggctatc taattgttcc agcaagccgg taaaattcag
      481 accgctaact gggtcgcggt agctattaac attttgggca ataatggtaa tttcttgact
      541 atcggcagcc agtaaatttt ggacttcagc tataatttca tcagctggcc gatatttttc
      601 ccggccacgg gcataaggga caacacagta actgcaaaaa ttgtcgcagc cgttgccaat
      661 cggcacgaga gcgctgattt tggaagtata aagcggttta atctgcagat aatcatggag
      721 atgggttaga ctgctggcgg gttgggggct gggtataatt tgctccaatt cggtcaattg
      781 gttgatagtg aaccaatagt ccacctgctg gccgatttta gcttgaatat ctggccgata
      841 cgacaaacag ccggtcaaag cgacggctgt ttgggggttg tcttgcttga tgcgcttgat
      901 taaaccataa atgcggtctt cggcgctttg acggacgccg caagtgacta agatagctaa
      961 atcagcttgc gatcgttgat tagtagctga ccaacccagc tcattaagac ggctggctaa
     1021 acgttggctg tcgctaatat tcatcgcgca gccgatagtg gtaatatgat aaagccaacc
     1081 catattaatc taataattct aaggcttctt tttgtttttt agttaaaccg gtcggcgtca
     1141 agaccttagc ctcaaccaaa tgatcgccgc ggccgtagcc gttaagttta ggtacgcctt
     1201 taccgcgcag gataattatt tgaccgcttt gggtgccggc tggaattttt aaagtcgctg
     1261 gcccatctac ggtatcaatt ttgattttat cgccaagagc tgcttgacgg aaacttagat
     1321 taactatgga ttggatattg tggccattgc gtttgaggtg gggatgcggt tggacgcgaa
     1381 cattaacata aagatcgccg cttttggcgc cacgccgacc ggcctcgcct tggccggtaa
     1441 atttaatggt ttggccgtta gctactccag ctggtatctt gatggttagt ggcttttttt
     1501 ccaccgttcg gccttggcca tggcaatgtg gacaaggttg atcaataatc tgtccctggc
     1561 cgttgcattt agggcaagtg gtttgagtgc gaatattgcc taacatagtt cgttgaaact
     1621 gatcgatcac tccccgacct tggcaatttg gacaattctt gggcgagctg cccgtcttgg
     1681 cgccgctacc ctgacattta ggacaagtag tttgtttttc cagattaata acttcttcgc
     1741 cgccaaagac cgccgttaag aaatcaattt ttaggccgac ttccaaatcg cggccgcggc
     1801 cgctatcggt ccgcgaggag ctacggctaa aaccgaagag atcaccaaac atttcaccaa
     1861 gatcgccgaa gttggcttca aaaccaccgg cttggccgcc ttgataaaat tggctaaaat
     1921 caaagccgcc catattggca ctattaaagc cgccagcgcc gccttgaaaa gcggccgaac
     1981 cgaagcggtc atatttagcc cgtttatctt ggtcacttaa gacttggtag gcttcattaa
     2041 tttctttaaa tttagcctcg ttgccgctag ctttatccgg atgatattgg tgagctagtt
     2101 tgcggaaagc ctttttgatt tcttcagccg aagcggtctt gctgacccca agaatttggt
     2161 aatagtcagt cgccatgaaa ggttattatt ttttattctt cttcgatttc accttcaact
     2221 ggaccattag cttcagtgcc ggccgttgaa tcggtagcgc tgtcagcttg attagcgcca
     2281 gcgctattag cttcggccgc tgtctgttga tacatagccg cgccgatttt ttgggcgacc
     2341 tcattggttt cttctaattt cttttttaag gcctctaaat cgctagcatc tttagcggtt
     2401 tttaattcgg ctatttttgt ttctagctca gtcttatctt cagctttaat tttatcagcg
     2461 gaatctttga gcagtttttc caattgaaaa ataacgctat cggcttggtt ttttagttca
     2521 gctgcttctt ttttctgctt gtcttcttca gcgtgctttt cggcttcttg acgcagtttt
     2581 tcaatctcat ctttacttaa accagaggaa gcggtaatag taatcttttg ttctttaccg
     2641 gaagccttat cggttgcctt aacattcaat ataccattgg catcaagatc aaaactaact
     2701 tcaacctgag gtacaccacg cggagctggc ggaataccat ctaaaataaa gcggcccagt
     2761 gatttattgt ctgttgccat aggccgttcg ccttggacaa tatggatttc cacgctagtt
     2821 tgaccgtcag cggccgtaga aaatacttgg ctcttagaag ttgggatagt ggtgttgcgt
     2881 tcaattaatg gtgtagccac gccacccagg gtttcaatgc cgagggtgag cggagtgaca
     2941 tctagaagta aaacgtcttt aacatcacct tgcaaaacac cagcctggac agctgcgcct
     3001 aaagcgacaa cttcatcagg attgacgctt aaattcggct ctttaccaaa aaattcttta
     3061 actttagctt gaaccatcgg cattctggtc atgccgccaa ccataataac ttcttcaatg
     3121 tcgccaactt gatagccagc gtcagctaga gctttttgac aaggctcaat tgttttggcc
     3181 accagatcgc cgaccaagtt ttccaactgg gctctggtca ttttcatgac taagtgtttg
     3241 gggccgttgg cgtcggtcgt gataaacggt tggttgattt cagtttcatt aagagtagat
     3301 aattcaatct tggctttttc ggccgcttct ttaattcttt gtagagccag agtgtcttta
     3361 gataagtcaa tcccctgatc ttttttgaat tcatcaataa tccaagtgat aattcgttgg
     3421 tcaaaatctt cgccgcccaa atgggtgtcg ccattggttg ccttaacttc aactgtatct
     3481 tcagaaatat ccagaataga aatatcaaaa gtaccaccac ctaaatcata aacagcaacc
     3541 tgctggccct tttttttgtc aaaaccataa gctaaggcag ccgcagtcgg ttcgttgata
     3601 atacgtttca catctagacc ggcaatttta ccggcatctt tagtagcttg gcgctgggaa
     3661 tcatcaaaat aggccggtac agtgataatc gcttcagtca cagtttcacc caagcgagct
     3721 tcagcatcgg cttttaattt agccagaatc atcgccgaga tttcttgcgg cgtatattct
     3781 ttatcttgca gtttaacttt aataccacca ccagcttgaa caatggaata tggtacattt
     3841 ttaaggtcat gctgaatttc ttcatcttgg aagcggcggc caatcaggcg tttgacagca
     3901 taaacggtat tttctggatt agttactgcc tggcggcgag cggtctgacc aattaatcgt
     3961 tcaccgtttt tggaaatagc caccaccgat ggggtagttc gattaccttc agcattctct
     4021 aagattttgg gttgcccggc ctccataatg gctactgcgc aattggtggt acccaaatct
     4081 attccaataa tcttgctcat aatcgttata ttgttttaaa ttattaatta ttttttcttg
     4141 ctttcaattt taatagtttt aggttgagct tctggagctt tggggatcat aatttttaaa
     4201 acaccatctt cagctactgc cttagtttca ctgcttaaaa catgagccgg cagctgaata
     4261 ctacgataga agctaccgcg gcggatttct ttgcgataat aatttttttc atcaacttcg
     4321 ctgtggcgtt cgccttcgcc tttaatgcat aaaatgtcgt tatcaatcga aatttggaca
     4381 ttttccggct ggataccggc taattgagtt tctacgataa cattgttttt atcttcatac
     4441 atatcaatgg ccggcacgaa attgtgctgg tcattacgta aggctggtaa gaagtcgtta
     4501 aagacttttt cggcttccgc caaactgtca aataagggat tccattttat tagtcctctc
     4561 atattgttgt ttttaattaa attaattaaa ttatatttat taattagttt aagtgatgtt
     4621 attgtttaaa gcgataaact ttaacccgag ccggcgtaat cactctgtct ttcagctggt
     4681 agccgggctt gatttcttca gccaccaaat tatcttgctg attgtcagcg gtttcaacca
     4741 tttcagtcgc atccatagtg gtatggtcaa atggttggtc aatggtcgga atttctttaa
     4801 ctccctgctg gcttaaaata ttttgaaatt gtttgataat ataattgata ccctctaacc
     4861 agctggcggt attatccgga ttaaagtggc ggcaagatag cttgagattg tcgtagaccg
     4921 gcagaatttc taagagcaat tgctcgttgg cgtaatgaac ataatgggcc cgatctttct
     4981 ggtgctgttt gatcaaattt tgttgctcgg ctaaggcgcg gcgatattta tcttccagtt
     5041 cgtgatattg ttcgtgacta taaccatgtt tagttttgct tttagtttct ttaacatttt
     5101 tcttggatgg ctcggtagac ataattgatt agattaaaat tagtctggta atcggttcta
     5161 agtggtgcca gcaagccaaa gaggccgatg tgctggtcga aacgatattt agttaaaatt
     5221 gtactggaaa aagcgccaaa agggctatcg ttacctagcc ggatggttgg ctcttcaccg
     5281 atctggttaa acaaatcgtc aatgatttct tccaaactgt caatgacctg agaaatatca
     5341 tagatcatgg ataattgttt aaactctggc tgaccaagta agccgcttaa tccggtataa
     5401 taagaatcat agcgatgaaa agaccaaaaa acgccgctat tagttaattg ggccagcgct
     5461 ttagctagcg gtttccaatt gtggccaaaa tcagctaaaa ctggttccaa cagcttttta
     5521 acggtactgg tcggtgcttt cggttcaatc gtagcaatta attggcgata agctaattcg
     5581 gttggaatac ggccggcgga ggtatgtggt tgaattatat aaccagcctc ctctaagtcg
     5641 gctaagtcgt tgcggactgt tgccggtgag aaatttaaat gatatttgtc tactagagct
     5701 tgagaagcta ctggtgttgc cgtttggata tagtcttcag tcaagagctt aagaattaag
     5761 gctggtcgtg tttccataga tatttttcat agttatcctc agtatatcat cgttagcagt
     5821 caactgtcaa gagtgctaaa ataatttgca aataaacaat aaaaagcccc agttagggct
     5881 ttttattatg gtattcatag cttatttaaa tacctaattt attgatgata atttcttttt
     5941 gactgtttaa gatctgatgg agaaaaatat cggccatatt ttggcgatat tcaaattcag
     6001 ctttgtccat taaagtataa cggatattgc ggccagttcc ctgctcaatt tttttaatca
     6061 agtcgttgag tttagtttta ttaaagtggt cgctaataat taaaatatca gtcggtgccg
     6121 ataaatcatt agtaaaaaag ccggttataa ttaatagttc aatagcgcct aattttnnnn
     6181 nnnnnncgtt gcgcagcttg ctctcttgac cgatctcggc tttagctaac aaagcgcgca
     6241 attcggccag cagataaaag ttttggttaa cttgataaaa cttcttgtcc agcttttgct
     6301 ggtggtcggt tttaacttct aatagaccga tctcttccaa attggccaat tcacgacgca
     6361 ctgaattgag ctgcaaatca agctcccgcg ctagttgtcg gatataaaaa caatcatctg
     6421 gtccggaaaa taagaaaagt ttaagcagtt tgagccgagc tccagagcta aaaagttttt
     6481 ctagcatata attatttaag gcttgagttt taggctgtat aaattttgtg ttagcccttg
     6541 tctaaaaaca taatatctta tataattatt ttcgtcaatc tttttaactg aagaaagtaa
     6601 tttatttttg gttaacctca gcctatttgt ttatgccata taagctcgcg tcgttattat
     6661 tgagctctga acaaaaaagc agttcaattg ctaaaattgc ttgggccgaa gccgatcagg
     6721 gcgatccggc gaacgaggtt cgtttttctc gggtcggtaa aatttttagt ctggtagaag
     6781 tttatgccac taacgccaaa gcactgctgc tgttggattt ttttagcgag cggccgaaat
     6841 tgaattatta tcagcagccg gatttggata agaatgaaca ccctggccta gatgatttgg
     6901 aaaaattttt taccgccgct attcaacaga ctaatcacga tctagccgct tttttagctg
     6961 agcataaaat cagttatctt gagccagagg ttagcgccac gattggttta ttaacagaac
     7021 gcggtttatt tttgacttca atcggccgca atcaggcctg gatgttattg cctggtcaag
     7081 atagcggtaa gcaagttgtt aagatgattg ttccggccga cgacggcgct gaacgaaccg
     7141 gccgtttatt tagcgaaata tttagcggca ctttacctaa tggtaattat tgtttaatca
     7201 gcaatgaggc cttggcggaa tatattgaac cggccacggc ggtaaactta ttaaccaata
     7261 aatcgccgtt agttggtgct gaaaaattac gtcatttctt gcaacagacc aatcgtcggg
     7321 tagatttttt gggtttaatt attaagcgct gtcatcgcga tgaagatgat gaagcttgtc
     7381 tggaaatgcc agctggtctg gtaagcaata atcagtcaac cactattgcc actccactca
     7441 taagtaataa tctgcgcttg actgaagaac gaaccgaaaa gattctcaat ccatctggct
     7501 ggtccaagtt tattttatta tttaagaaaa taataaattc tgtctggcaa tggttaatcc
     7561 gtttaagtcg tcgagactgg gttaaagttt ggcgcaataa ttatcacaat ggtcgagcat
     7621 actaccaaga aactaaaaaa aagattagag cttggcgcgg ttggccaatt attggtcgct
     7681 atagccaccg tctactatta atcggtcgat cggcttggta taagatccag ccttggtttc
     7741 gtcagattaa taatcgttgg acagcctatt ggtttaagac ggcgccttgg cgttgccgtt
     7801 tagtggaaaa atattggttg ccactggaac gaattattcg gccgcttatt aatttttttc
     7861 aagctgtcag tcatcgcgtt cgttgttttt tcaataaggg ctatcaacaa ccgcggactt
     7921 attggcagcg gcttagtaag cgcagccgga tatttttagg cctggctggc gtttgtctgc
     7981 tgctattgat tatcggcatg ttttggcagt atcgccgcca gataagacag gctaattggc
     8041 gacaaaaaac cgatcaagtc gctttggttg aacaaaaaca taatcaaatt gaagctaatc
     8101 ttttatataa taatctaact tcggctaatc agagcttgga tgaagccgct gccctattgg
     8161 aacaatggca agccgccaaa gaacaattac cggccgacat tactgagcgc tggcaaaatg
     8221 ctttattgaa gcaggaaaaa ctattaggtc aagttcggca tatttatact ttaagagatg
     8281 gtcaggcctt ggctaatcag ccgatcgcta ttgttacttc cacggatagc ttattgagat
     8341 taaatggtca attactggct tggactgatt cggctagtcg gcaagtgatt tatcaaaata
     8401 gtcgccaaga atggcaaacc gttgcttggc cggaaggctg gcagattatc gtggctgcgg
     8461 ccgctgatgg tcagagctgg tattttgctg ctggcgatgg gcaaattgct tcgctgagtc
     8521 gcgatggcca atggaaaatt tggtcgctgg acaagccaat cagccaaatt aaagcgttgg
     8581 ctttttatgg cggccgtttc tatgttgtgg ctggtgataa tcaaatctat cgctaccaaa
     8641 agcaagccga taattttacc agtggtcagc aatggaatac tgatcaattg ccggctggcg
     8701 atattgtcgg cctagtcgtt gacggccaag tttatgtttt ctatagtgat ggccaagcgt
     8761 ttaaattaaa taatggccgc agccaaaaca ttgccattaa gatcgcggag ccgactacag
     8821 agggctggtc cagttgtcaa ttattggctg atagcgataa tttttattgt ctagagtctg
     8881 gatatcggcg gatactactt tatggcaaag acggtgccat ttctggccaa taccgactag
     8941 ctgatcgtca agctcaagct gtggctatta gctcggcgag caaagctatt tatttattaa
     9001 ctgatcaggc gatacagaaa ttcgttttac caactgaata aacaatatta tataacaaaa
     9061 aagagctcgc tcggagctct tttttgttat tcgagagtca gcgcttattt taaggtgacc
     9121 ttgccgccag cttcttctaa cttagcttta atggtttcgg cttcttcttt cttaacgcct
     9181 tctttaatca tttttggagc ggcatcaacc aagtctttag cttcttttag gcctaaatct
     9241 tggttgattt cgcggatagc cttgatgaca gcaatcttat tggcaccagc atcgctgagc
     9301 tcaacatcaa agctatcctt ctcttcggct ggagcagcct cagcaccggc aaccgggcca
     9361 gccatcatca tagctggagc agcggcgctg acgccgaatt tcttttccaa aattttgacc
     9421 aattcggcca aatcaatgac ggacattttt tcaatttcgt ccaccaattt ttggaattta
     9481 gcaggaactt caatctgttc ctcagttttg ttcatttctt cagtcatagg attaaattaa
     9541 ttccgtagat tacggagcgg ttattaagct ttttggtcag cgaccgcttg cagaacagtc
     9601 accagaccgc gtaaattacc agccaataca ttaacaaagc cgctgactgg cgcgtttaag
     9661 gcgccgacta agcgagcttg taattctaac ttgcttggca agttggctaa cgactggaca
     9721 tcagcagccg acaaaaattg gccatctaaa ataccgccca ggaaggcaat tttgtcttca
     9781 tgggtttttt ggaatttttg tagcagtttg gccggcaata cctgatcgcc ataggcgaaa
     9841 ataatagcca cctgaccgtc aaattctctg acattcggtt gttcggtcaa ctggatattg
     9901 tttaaagcgc ggcccagtaa agttttttta atagccaaga attcgccttg ttcttccaga
     9961 agctgaccac gcagctcttc agtgtcccta acagtcaaat tattaaaaga agcgaagacc
    10021 acagctttgg attgagctag cttggtatcc agatcgcgca ggataatttc tttctgttgt
    10081 ttagtttttg gcatagtcgt tgcatgaaaa taaaaaaact gccatgtaac aatcgcagtt
    10141 cgacctttgg ggcagagtca aaaatatttt tgtctcggca ggttttacgc ctaagccacc
    10201 tgctgtctgc gacgaagtat tacattattt ttatcttaaa gggaaataat taatctgtca
    10261 agtttacgaa acttcggttt gactgataaa gcaggtttat ttatttcaaa tatttagctt
    10321 tattagcttt gtgctcttca aaattacggc taaacactgt ctcaccgtta tcggggcgag
    10381 ttaagaaaaa attataatca ttactggttg gataaatagc agcattaaga gccttgaggc
    10441 tggggctact gattggcccc agtggcaggc cgtcattaat ataagtatta tagggagaat
    10501 caattcgggt ttcttcgtag gaatattgcg gtttattttc accaagaata taagctaaag
    10561 tggcgcatga ttctaggcgc tggccgtttt taattcgccg ccagaaaata tccgcaacta
    10621 ttttcatatc tttttcgcta cgcacttcct tttctagtaa agaagccatg gtaacaatct
    10681 ggtaaattgt ctggccttgg cgtttaatat cttgacgcat ttttgtgttc agctgacggt
    10741 caaagttatt taatagttta ataatcacct cttctggact tgatgatgga tggaaacgat
    10801 aggtatcggc aaataaatag ccctccaggc ctagatattt cggcttatct tgtagaaact
    10861 catagcggtc ggaaaaatcc tgcggccatt tattaactgg cagtgattgg taattttttt
    10921 tgggataacc gctaatttgt aaaaaatcgt cagcgctgaa attaagctgg ctatcggcta
    10981 atttattggc aatctgctgg ctattccagc cttcaatcag ggttactaag cgatcggctt
    11041 gttgattggc ctgttcttgc caataattaa tccgtgcccg gcggttctct aactttttag
    11101 ttatataata aagtccgctg aagattccgc caaagataat aattgaaact aaccaaacta
    11161 gataaacagt tgactttttt tgcggtggat aattgttata ataagttgac atttagagat
    11221 atttatctag tgattctttt tttaaatcat caataatctc tttggtattg atagaatttt
    11281 ttagcgatga aataaaaaga acatcaccag tatcaataat agctaaatcg ttaacatcgt
    11341 taattactat tagtttgtca gtcaaattgc agtatactag attattattg gattttttag
    11401 ttattactcg tcctttggta acattatcgc tgacttcggt agctagaata ttttttagaa
    11461 ctggccattt gcctaagtcg ctccagccaa cgcggttgga tacactcatc gctagcttat
    11521 ccgccttatt agtaatagct gattcaatag aaattttatc taattggcca taaaggcggc
    11581 ggactttttc gttgtcgccg taattaatta gcaaaccaac cacctcttga caaatctgat
    11641 acattgtcgg ctgtaacttg gcaaataagt cggcgatagt ctgtaagcgc cagacataca
    11701 taccagtgtg gcaataatag attcctgaat caatgagttg gcgacaataa tcttcattgg
    11761 gtttttcaac gacattacag actgtaaaaa ttgtttcctg gccaatgctt tgcaggttct
    11821 ggccagcttt aagatagctg tagccagtgt caacataggc tggtttaaca gcaggagtca
    11881 ggatataatc ggggttttgt tgtaaaaatt gttcgctaac cagcagcaag tcagcaaaag
    11941 cttgattgtc ggaaatataa tcatcagatg gcaaagacgc aataatatca tcaggctggc
    12001 aaaaatgttg caaataacaa acctctaaac acatcgccgg tccggtatta cgcgattcac
    12061 tttcaacaat aatattgtta agcttaattt ccgggacttc aactttaacc cgtttaacca
    12121 tcaattcatt aatggagata aataaatcat cgggttgttg caagatttca gctactcgct
    12181 cataggtagt gcgcaacatt gttttatcac caagtagcgc ttgaaattgc ttaggattat
//
LOCUS       DAOPAI010000020        12020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_5259_length_12020_cov_5.243460, whole genome shotgun sequence.
ACCESSION   DAOPAI010000020 DAOPAI010000000
VERSION     DAOPAI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12020
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5259_length_12020_cov_5.243460"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKV49_02790"
     CDS             <1..282
                     /locus_tag="PKV49_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22569.1"
                     /translation="HWSYGLKYKLLKKQMASLATNYNYQFGRLLAAKPQINDYQQMRY
                     LAEKFFIIMTKFLYRSYTPRQRRLAAEQLRQLGIESPYQIYWRFHHGKS"
     gene            269..901
                     /locus_tag="PKV49_02795"
     CDS             269..901
                     /locus_tag="PKV49_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22570.1"
                     /translation="MENLKSQARLLQMARRQASDNTNYQLERWQWSYIWNFFKKQRRL
                     IINLIILLFFQGIVEAALIAISRFWLGRPIGWQLSNFWLIFWLLAIGFFVIVYWALRQ
                     EKTFSVLLANSLRRRLVRSQLNRSLAKSQTKYKSQLLAKVYYHLPLASMGVTNSLFGI
                     VRWLVGFLVTILLAYWAGLTWWLVGLFLILFSVAIFLVPILLLVDIFHKK"
     gene            811..1974
                     /locus_tag="PKV49_02800"
     CDS             811..1974
                     /locus_tag="PKV49_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22571.1"
                     /translation="MASWFIFNPFFCCHFFGAYFIARRYISQEVTFYSRLIYYLDNNL
                     SDKEFLKIFHQEGQVLSHFDRLVALDSFFRIRRDVWLKASSRLLFIVLLLLVSFLQLN
                     SGNLLSLINLSDSHNFSRLFLIVYLSRLANEALRIGLYWFPAKLGLYLTIVRSVGQLK
                     FPQIFKIDKTIVFYSPKTKLFQQSAYYRRLSFSFKPGDRILFTGPNLSGKTRLARLLA
                     GQGAYLPKSIHVKIDGRRFNYCQWQEIFGGAYLLDQTLATTEKSLMEFLLGRDKQNID
                     LADIEKILAVVNKQKIIKDLVAADGNLNRSVRVIDNHSSFFALQAAYCLINRPALIVV
                     DNFWLDLGYNDIRVILNIMSQQLSDSIIICFSGQANKFLNYHQHYAIKAQEIS"
     gene            1946..3127
                     /locus_tag="PKV49_02805"
     CDS             1946..3127
                     /locus_tag="PKV49_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPL22572.1"
                     /translation="MPLKLRKSVKKLKITQLVQLIWLALFLIFIIFLVIWLKPQANLL
                     FNPFVFSEPVQQINEPFSGQLYFNDQLGQNKLSQLVIAAIDSAQATIELAVYSFSDQD
                     IKEAIYQAANRGVKVTIILDAGKSQFNNQLLSDLPENIRRIDIKSKRGYMHHKFIIID
                     RGQSSGQLLFGSYNFTKLQDRYDPSFLLISPRPELVDSFGREIDRLANNNYGRKKILA
                     SDCLAERFQYTNGFLEIWFGPQTRNRGLRNRMIDLIYGTKNSLKGMIWNFTDRDLAHA
                     MLTVARRHPVTMIIDDVNWTGKYSVNQIMTKSLFNKLEIITDHRRNQEVKDLSGQDDL
                     NSFLHHHALIIDDQTVVFGTNNWSSGGFFDNDESTIISNIDSLVQSFGQSFLFNYQKN
                     Q"
     gene            3579..5678
                     /locus_tag="PKV49_02810"
     CDS             3579..5678
                     /locus_tag="PKV49_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPL22573.1"
                     /translation="MENEKNSRLSQLKKLLLATRRLIQRRRIVFFLLVVIIVGGYYFY
                     HHKQAGISSTTYVMGTAEKGSIVVSVSGSGQIETSDQVDVKPNVAADIIAIYIKAGQK
                     VKAGDVIAKLDASDLKNKVTQANNSLTSARANLNLKLAGATKQEIKLAENSVASAQLS
                     YEQAVANVNDVKISGEQSLAKAQIQLDNAQISLENAQRNYDNTLATHNISGESDDNDL
                     SKTYSDAKSALESAQLTVRSAIVSADDILGKNNYSNADHPYLYLLGARDSQSIINANN
                     SYELARAEFIKLEASNRLAATTSWTNDDVEKQLIATKAALQSAKTMAHDVYNVLLNTV
                     VSAKLSQSTIDGYKQTVSSQESSLLNGINSINQAQQAINNIKLGISSSGLSSSASVNN
                     AKSSLDTAKNNLVTAQSSLVQAQSDNKKSLDSANSELASRKIAYENAKIQLEQKTAAP
                     REVDLASARVQVAQAAQDYEQAVQDLADAEVRSPIDGVVAKVNQKVGYAASASDSAAN
                     SIATIITDQQLAVISLNEVDIAKVKVGQQASLSFTAIDGLKLTGQVAEVESIGASEQG
                     VVSYEIKISLDYQDERVKPQMSVSAEIVIDSKTDILVLDNSLIKTDSQGLSYVEIFGK
                     RRLKLGETITTADLPEVKYIEIGLSNDTKSEVVSGLTADDIIVVQSISNANKTAPSAS
                     NQRGGFQMMGGMGGGPR"
     gene            5692..6366
                     /locus_tag="PKV49_02815"
     CDS             5692..6366
                     /locus_tag="PKV49_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPL22574.1"
                     /translation="MIELKKINKIYQLGDNETQVLKDISLKISDGEFVAITGPSGSGK
                     STIMHIIGALDTPTSGTYLLDGQDVSALSDDDLADIRKNKIGFVFQSFNLLSRATVLR
                     NVALPLIYAEIPLEKRQAMVEAALRACGLAQDRWHHLSNQLSGGQMQRVAIARALVNN
                     PSIILADEPTGNLDSKTGQVVLETFKKLNQDSGHTIILITHELEVAKQADRIIYIRDG
                     QIVGEI"
     gene            6363..7580
                     /locus_tag="PKV49_02820"
     CDS             6363..7580
                     /locus_tag="PKV49_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPL22575.1"
                     /translation="MMLTDLVKETILAVTANKTRTSLTMLGIVIGIGSVIAMVAVGQG
                     AQKSIEESIQSIGSNLLMVQPGPVRSAGVSAGRGNAKTLTIGDAEAIGSQIAKVKAVS
                     PELSSRYQVTAKGQNTNTQIIGVTADYASVRSLEIDLGSFIRSKDSLSASKVAVLAPT
                     TRDDLFGTSTNPVGQTISINKINFTVIGVTKAKGGSGFNNQDDSIFVPLKTAQLYLSG
                     NEYLSTISIQADSQDVLTEVQNQVSQLLLARHKITDSTKADFQIMNQADIVETASSVA
                     GTFTALLAAIAGISLLVGGIGIMNMMLTTVTERTREIGLRKSIGAKRRDISQQFLAEA
                     VLLTFLGGAIGIVFGWLTSLAIAKFANTATSISGSSVLLAFAVSAAIGIIFGYYPAQR
                     AAKLNPIDALRYE"
     gene            7600..8013
                     /locus_tag="PKV49_02825"
     CDS             7600..8013
                     /locus_tag="PKV49_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22576.1"
                     /translation="MKNKKNLLLALTVILALTISFWLGRLSGRGNANMALANRRQAGP
                     MAVGQRQGFGNQNTQNQIIGQIDKITDNQLAIKLIDGGSRLLMLPSEAVITKTASATS
                     ADLVVGQSITASGAEEGGVVLVKTLQINQAEPPRF"
     gene            8256..8846
                     /locus_tag="PKV49_02830"
     CDS             8256..8846
                     /locus_tag="PKV49_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPL22577.1"
                     /translation="MAEVINQNSLNELSDEQLIPLIIQDRNVFVVIMARYKIKLFNYI
                     QRLTNIGDDDAEDLLQDVFLKVYLNINSFNQQMKFSSWIYAITRNQVISRYRRLASRP
                     EGHAVVLDEQLVGRLAAGFNLVDQQDRKNIASVVSDGLYRLKSKYRDILILKYVEEKN
                     YQEISDIIRKPLGTVGSMLNRAKLELKKVIDRKQIL"
     gene            8857..9648
                     /locus_tag="PKV49_02835"
     CDS             8857..9648
                     /locus_tag="PKV49_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22578.1"
                     /translation="MIKEDNFEQEIMEQIERRQIHPRPQWLFVLKNLNFWLLGVVALL
                     LGSISTALLIYFFQGGAVSLHRRAGASPAELFLLSVPLFWVAITIGLIYWTYINVKNT
                     ERGYRYSPWLVGGVILLISLTAGFILFFCGFSRTIDNILGRRLPFYEHVINPRVGFWS
                     EIDHGRLSGIVIGQDHNGQGWLLTDHQGRVWQVGIGRFQAPGGQLLPDFNRVAGRPVG
                     FWGRKITDHQFEAREFFPLRSGEGFFGQPRPLINPPFAGPGPGRR"
     gene            9958..10383
                     /locus_tag="PKV49_02840"
     CDS             9958..10383
                     /locus_tag="PKV49_02840"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPL22579.1"
                     /translation="MKKSFYRTSSAFTLLELLIVFFIIALLSTLAYVSLENARAKGRD
                     TRRLADIHNIRQALELYKDIEGNYPEQLPAPQQSFVGSSGRSYLAPMPSDPKTRQPYA
                     YSSSATSGYHLIFSLEKDTLDYPAGENIITPVGFNSDDN"
     gene            10411..10539
                     /locus_tag="PKV49_02845"
     CDS             10411..10539
                     /locus_tag="PKV49_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22580.1"
                     /translation="MVVVSFSPTEWQLIVGITWRIVTAFLAWAVISNLTHAFTGTK"
     gene            10517..10981
                     /locus_tag="PKV49_02850"
     CDS             10517..10981
                     /locus_tag="PKV49_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22581.1"
                     /translation="MHLLAPNKNNGQRLIVNSHNDRQKITELNCARRWLTAYNKINQT
                     GYCCLKANYFQSEVDVYVYRDKEVGRPLRLQITKAKFDSLTAAIQRKKGLYSPEQKAD
                     LFLLLDFSGPISLNKLNVWQSKNLRLLSGSGFLEIWLVGSRGNVINLWPGLA"
     gene            complement(10985..11650)
                     /locus_tag="PKV49_02855"
     CDS             complement(10985..11650)
                     /locus_tag="PKV49_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPL22582.1"
                     /translation="MELNYQMVKNNQKINGFIRATEKYIIELGYTDHGFRHLTIVADR
                     AKKLARELGLTAKEQELAAVAGYCHDLGNFMGRDMHHYWSALLLSQILLPHIEQTADL
                     AKVLQAIVNHDNNNLETNNAVAAVLVIADKSDVHRSRVKQKSLRKIKEDIHDRVNYAV
                     NDNDLLINRQTKEITLKLTINTNWVDPLAYFQIFIERMTACQKAAKVFGYKFVLIINN
                     FRF"
     gene            complement(11655..>12020)
                     /locus_tag="PKV49_02860"
     CDS             complement(11655..>12020)
                     /locus_tag="PKV49_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPL22583.1"
                     /translation="FLVNPILGLTAAVAIFIHEIPQEISDFGILLHAGYSRIKAIKLN
                     FLSSLSAFMGALVAIVLSQDWQPTNLLAFTAGNFIYIAAADLFPEMQKETTWQKSSRQ
                     LIGLLIGLTIMLLMKIFLD"
BASE COUNT         3465 a         2117 c         2603 g         3835 t
ORIGIN      
        1 cattggagtt atggtcttaa atataagctg cttaaaaaac aaatggccag cttagccact
       61 aattataatt atcaatttgg ccgtttattg gcagccaagc cgcaaattaa tgattatcag
      121 caaatgcgtt atttagccga gaaatttttt ataattatga ctaaattttt gtatcgttcg
      181 tatacaccgc gacaacggcg attagcagcg gaacagcttc gtcaattagg tattgaatcg
      241 ccctatcaaa tttattggcg ttttcatcat ggaaaatctt aaatcacaag ctcgattgtt
      301 acaaatggct cgtcgacagg cgagtgataa tactaattac caactagaaa gatggcagtg
      361 gtcttatata tggaattttt tcaaaaaaca gcgacgacta ataattaatt taattatttt
      421 gttgtttttt caaggaattg tcgaggctgc tctgatagcc attagtcgat tttggttggg
      481 tcgcccaatc ggttggcaac tcagtaattt ttggttaatt ttttggttat tagcaattgg
      541 tttttttgtt attgtttact gggcattacg tcaggagaaa actttttctg tgcttttggc
      601 taacagtctt cgacggcgat tggttcgttc tcaattgaat cgctcgttgg ccaagagtca
      661 aactaagtat aaatctcaat tattagctaa agtatattat cacttgccat tggcttcaat
      721 gggggtgacc aattcattat ttggcatagt ccgctggcta gttggttttt tagttaccat
      781 tttgttggcc tattgggctg gattaacctg gtggctagtt ggtttatttt taatcctttt
      841 ttctgttgcc atttttttgg tgcctatttt attgctcgtc gatatatttc acaagaagta
      901 actttttatt cccgattgat ttattattta gataataatc taagtgataa agaattttta
      961 aaaatttttc atcaagaagg ccaagtgtta agtcattttg atcgtctggt agcgcttgat
     1021 tctttttttc gtattcgtcg cgatgtttgg ctgaaagcta gctcgcgatt attatttatc
     1081 gttttattat tattggttag ttttttacaa cttaattctg gtaatttatt atcgttaatt
     1141 aatttaagcg atagtcataa tttttcgcgg ctatttttaa tagtttattt gtcgcgcttg
     1201 gctaacgaag ctttacggat tggcctttat tggtttccag ctaagctggg gttatatcta
     1261 actattgttc gttcagtcgg ccaattgaaa tttccccaaa tatttaagat tgataaaaca
     1321 attgtttttt attctccaaa aactaaactt tttcagcaga gtgcttatta tcggcgcttg
     1381 agtttttctt ttaagccggg ggaccgaatt ttgtttaccg gtccaaattt atcgggtaag
     1441 actaggctgg ctcgcttatt agctggtcaa ggcgcctatt tacctaaatc tattcatgtt
     1501 aagatagacg gccgtcgttt taactattgt cagtggcaag aaatttttgg cggcgcttat
     1561 ttactggatc agactttggc gaccaccgaa aagtctttga tggagttttt gttgggtcgc
     1621 gataagcaaa atattgactt agctgatatt gagaaaattt tggcagttgt aaataagcaa
     1681 aaaattatta aagatttagt ggcagcggac ggtaacttaa atcgttcagt ccgagttatt
     1741 gataatcaca gtagtttttt tgctctgcaa gcagcttact gtttaattaa tcgaccagca
     1801 ttgattgttg ttgataattt ttggttagat ctgggctata acgatataag agtgatatta
     1861 aatattatga gccaacaatt gtcggatagt attataattt gtttttccgg tcaggctaat
     1921 aagttcttaa attatcatca gcattatgcc attaaagctc aggaaatcag ttaaaaaatt
     1981 aaaaatcaca caattggttc aattaatatg gttggcttta tttttaattt ttataatttt
     2041 tttagttatt tggttgaaac cgcaagccaa tctattattt aatccttttg ttttttccga
     2101 accggtgcag caaattaatg agccattttc cggacagctt tatttcaatg atcaactggg
     2161 gcagaataag ttatcccaac tggtcatcgc ggcgattgac agtgcgcagg ccactattga
     2221 attagcagtt tattctttta gtgatcaaga tattaaagaa gctatttatc aagccgccaa
     2281 cagaggagtt aaagttacta taattttaga tgctggcaag agccaattta acaatcagct
     2341 actgagtgat ttgccagaga atattcggcg gattgacatc aagtccaaac gtggctatat
     2401 gcatcataaa tttatcataa ttgatcgtgg ccaatcgtca ggacagctct tattcggttc
     2461 atataatttt actaagctgc aggacagata cgatcctagt tttttattaa ttagtccaag
     2521 acctgagctg gttgactctt tcggacggga gattgatcgg ttagccaaca ataattatgg
     2581 ccgcaaaaaa atattagcca gtgattgtct ggcagagaga ttccaatata ccaatggttt
     2641 tttggagatt tggtttggac cgcaaactcg gaatcgcgga ttaagaaacc gtatgattga
     2701 tttgatatac ggcactaaga atagtcttaa gggtatgatt tggaatttta ctgatcgtga
     2761 tttggcccat gcgatgttaa cagtcgcccg ccgtcatccg gtgaccatga tcattgatga
     2821 tgtcaattgg actggtaagt attcggttaa tcagataatg actaaatcgc tatttaataa
     2881 gctggaaata atcactgatc atcggcgtaa tcaagaagtt aaagatttat ctggtcagga
     2941 tgatttgaat tcattcttgc atcatcacgc gctaataata gatgatcaaa cggttgtttt
     3001 tggaaccaat aattggagtt ccggcgggtt ttttgataat gatgaatcaa caattatctc
     3061 taatatagat tcgcttgtcc agtcgtttgg acaatccttt ttatttaact atcaaaaaaa
     3121 tcaataaaca aacaaagtat agcactcttg gtttttttgg gcagcttgtt agctatcagt
     3181 tacttagctg ttaatttgat cagcattcta gcaacaatgc tattattggt attctcgaca
     3241 aatggggggt ggttggataa tctggaagat tagttatagt aatttagagc tattactcta
     3301 ggagaactaa cactgaatga ggcgttagcc agagtgcaaa atgtgtgtgc ctaacaacaa
     3361 gtattattag tcaagcatgg tgatgacttt tggaaatttt gtggtggtaa gttaattata
     3421 tcatgcggca caaagggaag ctaaagaaga gctgggtatt gacatcaaga ttattaaccc
     3481 agagccatta ttttaaaatt atttgaagat ttttttataa tctgcgtact aagagataga
     3541 ggtttaattt tataaaccaa ttttttggta tagtgattat ggaaaatgaa aaaaatagcc
     3601 ggttatctca attgaaaaaa ttattactag cgacccgtcg gcttattcag agacggcgga
     3661 tagttttttt cttgttagtc gtcattatag ttggcggcta ttatttttat catcacaaac
     3721 aagccggcat cagtagcact acctatgtaa tggggacggc tgaaaaaggt tctattgttg
     3781 tttcggtttc tggatcaggg cagattgaga cctctgacca agttgatgtt aaacctaatg
     3841 tggcagctga tattattgct atatacatta aagctggaca aaaagttaag gctggcgatg
     3901 ttatcgctaa acttgacgcc agcgatttaa aaaataaagt tactcaagct aacaattctt
     3961 tgacttcagc tcgagccaat ttaaatctta agttggccgg agctactaaa caagaaatta
     4021 aactggccga gaactcggta gcctcagctc agctcagtta tgaacaggca gtcgctaatg
     4081 ttaacgacgt taagatcagc ggcgaacagt cattagctaa ggcgcaaatc cagctagaca
     4141 acgctcaaat tagcctggaa aatgctcaga gaaattacga taatacttta gctactcata
     4201 atatcagcgg ggaaagtgac gacaatgatt tatctaagac ctatagtgat gccaaatcgg
     4261 ccttggagtc ggctcaatta acggttcgtt cggccattgt ttctgctgat gatattttgg
     4321 gcaagaacaa ttatagcaac gctgatcacc cttatctcta tttattgggt gccagagatt
     4381 ctcaatccat aattaatgct aataattcct atgagttggc gcgggctgaa ttcattaaac
     4441 tagaagccag taatcgctta gctgctacca ccagctggac taatgatgat gtcgaaaagc
     4501 aattaatagc tactaaagca gctttacaat cggccaagac tatggctcat gatgtttata
     4561 atgttttatt aaatacagtt gtttcggcca aattgagtca gtcaaccatt gatggttaca
     4621 aacaaacggt ctcttctcag gaaagttcat tgttaaacgg tattaatagt attaatcaag
     4681 cccaacaggc aattaataat atcaaactgg gcatatcatc ttccggacta tcctcttcgg
     4741 ctagcgttaa taatgctaag tcatcattgg atacagccaa gaacaattta gtaaccgccc
     4801 aaagcagttt ggtccaagcc caatctgaca ataagaagag tttagattca gccaactctg
     4861 aattagcttc gcgtaaaatt gcttatgaaa atgctaaaat acaactagaa caaaaaacag
     4921 cggcaccgcg cgaagtagat ttggctagtg ctcgggttca agtggctcaa gccgcccaag
     4981 actacgagca ggcagttcaa gatttagccg atgccgaagt aaggtcgcct attgatggtg
     5041 tggtggccaa agttaatcaa aaggttggtt atgccgcctc ggcctctgat tctgctgcca
     5101 attctattgc cacaattatt accgatcagc aattagcggt tatttcttta aatgaagtag
     5161 acattgccaa ggttaaagtt ggccagcaag cttcactcag ttttacggct attgatgggc
     5221 tgaaattaac cggtcaagtg gctgaggtgg agagtattgg cgccagcgaa caaggggttg
     5281 ttagctatga aattaaaatt agcttggatt atcaggatga acgggttaag ccgcaaatga
     5341 gcgtgtcggc cgagattgtt attgatagta aaaccgatat tttagtttta gataattctt
     5401 taattaaaac agacagtcag gggttatcct atgttgagat attcggtaag cgccgcttga
     5461 aattgggcga gacaattact actgccgatt tgcctgaagt taaatatatt gaaatcggtc
     5521 tgtctaatga caccaagagc gaagttgtta gcggtttaac cgcagatgat ataattgttg
     5581 ttcagtctat cagcaatgcc aataaaacag ccccgtcagc tagtaaccag cgtggtggtt
     5641 ttcagatgat gggcggtatg ggtggcggac ctcgctaagt aattattttg tatgattgaa
     5701 ttaaagaaaa tcaataagat ttatcagctt ggcgataacg agacgcaggt tctaaaagat
     5761 atttcgctta aaattagcga cggtgaattt gtcgccatta ccggtccgtc tggatcaggt
     5821 aagtctacga tcatgcatat tatcggggca ttagacacgc cgactagcgg cacttatcta
     5881 ttagacggcc aagatgtttc agctttgtcc gacgatgatt tggctgatat tcgtaagaat
     5941 aaaattggtt ttgtttttca gtcatttaat ctcttatctc gagccacggt cttgcgtaat
     6001 gtggctttgc cgttaatcta tgccgagata cctctagaaa aaaggcaagc aatggtggaa
     6061 gcagccctgc gggcttgcgg cttagcccag gatcgttggc accacctatc taatcaatta
     6121 tctggcggac agatgcagcg ggtggctatt gccagagctt tagttaataa tccatcaatt
     6181 attctggctg acgagccgac tggtaatctg gacagtaaaa ctgggcaggt ggttttagaa
     6241 acttttaaga aattaaatca agactctggt cacaccatca tcttgattac tcatgagtta
     6301 gaagtggcca agcaagctga ccgaataatt tatatccgtg atggacaaat agtgggggaa
     6361 atatgatgtt gactgattta gttaaagaaa ctattttggc tgtaactgcc aataaaacca
     6421 gaactagttt gacaatgctg ggcatcgtta tcggtatcgg ctcagtaatt gccatggtgg
     6481 cagttggtca aggggcgcag aaatccattg aagagagtat ccaatcaatc ggttcaaact
     6541 tgctaatggt tcaacctgga ccggttcgtt cagctggtgt tagtgctggt cgaggtaatg
     6601 ccaaaacttt aaccatcggt gatgccgaag ctattggcag ccaaattgcc aaagttaaag
     6661 ctgtttcgcc agaactatct agtcgctatc aggtgacggc caaagggcaa aataccaata
     6721 cgcagattat tggcgtaact gcagattatg cttcggttcg cagtctggaa atagatcttg
     6781 gttcatttat cagatcaaaa gattcactta gtgctagcaa ggtggcggtc ttggcgccga
     6841 ccactcgcga cgatttattc ggcactagca ctaacccagt tggtcaaaca atcagtatta
     6901 acaaaattaa tttcacagtt atcggagtca ctaaagccaa gggcggcagc ggttttaata
     6961 accaggacga cagtatcttt gtgccgctta aaaccgctca gctttatctg tccggtaatg
     7021 agtatctaag cactattagt attcaagcgg atagtcaaga tgttttaact gaagtccaaa
     7081 atcaggtcag tcaattatta ttggctcgtc acaaaattac tgactccact aaggcggact
     7141 ttcagattat gaatcaggcc gacattgtgg agacggcttc gtcggtggct ggaactttta
     7201 cggctttatt ggcggcaatc gctggtattt cattactggt agggggtata ggtattatga
     7261 atatgatgtt aaccacagtt acagagagga ctagagaaat tggccttaga aagtctatcg
     7321 gagcgaaacg tcgggatatt agccagcaat ttttagccga ggccgtttta ttaacttttt
     7381 tgggtggagc tatcggcatt gtttttggct ggctgacttc gctagccatt gctaaatttg
     7441 ccaatacggc tactagtatt tccgggtcat cagtgctact ggcttttgcc gtttcagcgg
     7501 ctattggcat aatttttggt tattatccgg cccagcgagc cgccaagctt aatccgattg
     7561 atgccttgcg ttatgaataa ttattaaatt aagaataata tgaaaaacaa gaagaattta
     7621 ttattagcct taacagttat attagcctta actattagct tctggctggg acgattaagc
     7681 ggccgtggca atgccaacat ggctttagct aatcggcgcc aagccggacc aatggcggtt
     7741 gggcaacgcc aaggttttgg taatcagaat acccaaaatc aaataatcgg tcagattgat
     7801 aagattactg ataatcagtt ggctattaaa ttaatagatg gtggatcgcg tcttttaatg
     7861 ttgccatccg aagcagtaat cactaaaacg gccagtgcca ctagcgcaga tttggtagtt
     7921 ggacagagta ttacggctag cggtgctgag gaaggcggtg tggtcttggt taaaacatta
     7981 cagattaatc aagcagaacc accgcgtttt taattggaat ttataatatt ttttgttata
     8041 ctaaataagt attctttaga atcaccccca tattattagc caccaaaccg cacattaatg
     8101 attatcaacc agttcgttat ttatctgaag aatttttaaa ggttatggtt attttatata
     8161 gtgcgtatac gtcgcttcat cgacggttag ttagggaatt atcggccgaa ttgccaacag
     8221 gcagccgatt atataattag ataaatatct atctaatggc tgaggttatt aatcaaaaca
     8281 gtttgaacga attgtctgac gagcagttga ttccgttaat aatacaagac cgcaatgttt
     8341 ttgttgttat tatggctcgt tataagatta aattgtttaa ttatatacaa agattgacca
     8401 atatcggcga tgatgatgct gaagacttac tgcaggacgt ttttttgaaa gtctacttga
     8461 acatcaatag ttttaatcag cagatgaaat tttcttcctg gatttatgcc ataacaagaa
     8521 atcaagtaat cagtcgctat cgtcgcttag cttcccggcc cgaaggccat gccgttgttt
     8581 tagatgagca gttggttgga agattggcag ctggttttaa tttagttgat cagcaagaca
     8641 gaaaaaatat tgctagtgtg gtatcagatg gattatatag attgaaatct aaatatagag
     8701 acattttaat ccttaaatat gttgaggaaa agaattatca agaaatttct gatattatta
     8761 gaaagccatt gggcacggtg gggtcaatgc ttaatcgggc taagttagaa ttaaaaaagg
     8821 taattgatcg caaacaaata ttataaataa ttatatatga tcaaagaaga taattttgaa
     8881 caggaaatca tggagcaaat tgagcgccgt caaatccatc cgcggccaca atggctattt
     8941 gttctaaaga atctgaactt ttggctattg ggagtggttg ctttattgct tggatcgata
     9001 tctacggcgc tcttgattta tttctttcaa ggtggggcag taagcctaca tcggcgcgct
     9061 ggagccagtc cggccgagct ttttttattg tcagtgccgt tattttgggt ggcaattact
     9121 attggtttga tttattggac ttatattaat gtcaaaaata ctgagcgcgg ctatcgttat
     9181 tcgccatggt tggttggtgg agttattttg ttgattagct tgactgctgg ttttatttta
     9241 ttcttttgtg gttttagccg gacaattgat aatattctgg gacgtcgttt gccattctat
     9301 gagcatgtca ttaacccgcg agttggtttt tggtcagaga ttgaccatgg tcggctgtct
     9361 ggcattgtta ttggccaaga tcataatggt cagggctggc ttttgaccga tcatcaagga
     9421 cgggtctggc aggtgggtat cggtcgattt caagcgccag gtgggcaatt attgccggat
     9481 ttcaatcggg ttgccggtcg accagtcggt ttttggggaa gaaaaattac tgatcaccag
     9541 tttgaggctc gagaattttt tccattgcgc agcggtgaag gttttttcgg ccaacctcgc
     9601 ccgctaatta atccgccttt tgctggtccc ggacccggtc gtcgttaatt tatatttttt
     9661 aaaaaataaa ataccgttaa agtaaagcgg tattttttat ttccctacca aaaagggaca
     9721 ccccaagtgg tcaaactatt gccaagcgct tcaagggagt gcccaatcag cgaggggggt
     9781 gccccttacc ggtctgattg ggttaattgc cttaaataaa cgccaatttt tggctgccac
     9841 tggcaatagt ttgactggct ttattctatt atcaaccgag atgattgtca attaaccaac
     9901 tatttggcga taaattattt tttatgtata atatcattat aaatctaatt attgtttatg
     9961 aagaagtcat tttaccgcac tagttcagcc tttactctgt tggaattatt aattgttttt
    10021 tttatcattg cgttactttc gaccttggcc tatgtttcgt tagaaaatgc tcgagccaag
    10081 gggcgtgata cgcgtcgtct ggctgatatt cataatattc gccaagcgtt agagctttat
    10141 aaagatattg aaggcaatta tccagagcaa ttacccgcgc cacagcaatc atttgttggt
    10201 tcaagcggcc gttcctatct ggcgcccatg cccagtgatc ctaagactag acagccttat
    10261 gcttacagca gttcggctac tagcggctat catttaatat ttagcctaga aaaagatact
    10321 ttagattatc cagccggtga gaatattatc actccagtag gctttaattc agacgacaat
    10381 tagtgtgttt attgtttctt aagtgttctt atggtagtgg ttagttttag tccaaccgaa
    10441 tggcagctga ttgttggcat tacttggcgg atagtcaccg cctttttagc ttgggcggtt
    10501 atttctaatt taacccatgc atttactggc accaaataaa aacaatggcc agcgcctaat
    10561 cgtcaatagc cacaatgatc ggcaaaagat taccgaattg aattgtgctc ggcgttggtt
    10621 aacagcctat aataaaatca atcagactgg ttattgttgt ctgaaagcta attattttca
    10681 aagcgaagtg gatgtttatg tctatcgaga caaggaagta ggtcggccgt tgcggttgca
    10741 gattactaaa gccaagtttg attctttaac agcggccatt cagcgcaaaa aaggacttta
    10801 ttcgccagag cagaaagccg acctattctt gttattagat ttttctggac cgatttcgct
    10861 gaataaatta aatgtttggc aaagtaaaaa tctccgatta ttatcgggca gcggtttctt
    10921 agaaatttgg ctggtgggct caagaggtaa tgttatcaat ctgtggccgg gattagctta
    10981 attgctaaaa acggaagtta ttgataatca ggacaaattt atagccaaat actttggcgg
    11041 ctttttggca ggcagtcata cgctcaataa agatttggaa gtaggccagt ggatcaaccc
    11101 aattggtgtt aatggttagt tttagggtaa tttctttggt ttggcggttg attaataaat
    11161 cattatcatt gacggcataa ttaactcggt cgtggatatc ttctttaatt ttccgcaagc
    11221 ttttttgttt aactcgacta cggtgtacgt ctgacttatc agcaattact aaaacagcgg
    11281 cgacagcgtt attagtttca agattattgt tatcatgatt aacaatagct tgtaatactt
    11341 tagctaaatc ggcggtctgt tcaatgtgcg gcaggagaat ttgtgataac aataaagctg
    11401 accagtaatg gtgcatgtcg cgacccataa aattgcccaa gtcgtgacaa tagccagcga
    11461 cggcagccaa ttcttgctct ttggcggtta agcccagttc acgggccaat tttttagctc
    11521 ggtcggcgac aatagttaga tgacgaaaac catgatcggt atatcccagt tcaataatat
    11581 atttttcagt agctcggata aacccattaa ttttttggtt atttttaacc atttggtagt
    11641 ttaattccat aattttagtc taaaaagatt ttcattaata gcataattgt taagccgatt
    11701 aataagccga ttagttggcg actagatttt tgccaggtag tttctttttg catttcgggg
    11761 aataagtcgg cagcagcgat ataaataaaa ttaccagcag taaaggccaa caagtttgtc
    11821 ggctgccaat cttgacttaa gacaatagcg acaagagctc ccatgaaggc cgataacgac
    11881 gataagaagt taagtttgat tgctttaatt cgagaatagc cggcatggag cagaatacca
    11941 aagtcgctga tttcttgagg aatttcatga ataaaaatag caacggcggc agttagtccc
    12001 agtattggat taaccaaaaa
//
LOCUS       DAOPAI010000021         8261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_8007_length_8261_cov_4.388496, whole genome shotgun sequence.
ACCESSION   DAOPAI010000021 DAOPAI010000000
VERSION     DAOPAI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8007_length_8261_cov_4.388496"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKV49_02865"
     CDS             complement(<1..260)
                     /locus_tag="PKV49_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22584.1"
                     /translation="MGNCLARKYKTLITGSIILIVALLLIGHSVLAASVTTSSNESSG
                     ALKFTPQVGLPGFMSEYVFTENSTMPIAKLMGSLFKYGIQIIG"
     gene            complement(245..1420)
                     /gene="porA"
                     /locus_tag="PKV49_02870"
     CDS             complement(245..1420)
                     /gene="porA"
                     /locus_tag="PKV49_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HPL22585.1"
                     /translation="MKPITSNDSAGQLTFLKGSEAIAKMVALCQPGVVAAYPITPQTG
                     IIEQLVKLSASGQADFSFVQAESEYSALSIALGAAAMGVAAYTATSSQGLLYMTEVIY
                     NLAGLRLPLVMTCANRSISAPINIWNDHQDAMAVREAGWLMFFAEDNQEAIEQHWLAF
                     ALSQKLRLPVMVNVDGFRLTHASEPVALPNKQLVKKLLNNKIKAVLNTKKPQTTGWFA
                     MPTEYQQQRLELRRVIANSWPIIKNTNQQFNNLWQPIKTKLLGRNNHSSDPAVEYYGP
                     AQAKLVIVAMGSIIGELKDLADKANKQKPGSVAILKIKLYRPFPEKVIERYLNQAEQI
                     IVIDRSPGLGAQPPLFSDIMTIKPLNKNNISSQIIGLGGDIDLIKINKTIKNIWAIV"
     gene            complement(1417..1977)
                     /locus_tag="PKV49_02875"
     CDS             complement(1417..1977)
                     /locus_tag="PKV49_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPL22586.1"
                     /translation="MAALTKKLNRSITIKILGLGGQGVVTTAATLARAAFHQGYWSQS
                     MPFFGVERTGTPVEAYVRIADQSIRNRQKINRCDILIINDYRLYQQNDLGKQLTIINH
                     SDLANYQPKTKLLTYDSRQISLDIFNQDLAIGLLGGLSAAWSLIDEDNWFKALSEQFA
                     NPIIAQKNQQAFLANRQLAIKLIKSA"
     gene            complement(1977..2789)
                     /locus_tag="PKV49_02880"
     CDS             complement(1977..2789)
                     /locus_tag="PKV49_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPL22587.1"
                     /translation="MKKNYKSKPLPITEGSSGWQNRFNALLRSSGRRPRRWYRRWWGI
                     IILIIAALFSLFVAVYLYKVVILVWQLKTGRITAAELMLQQSNPGLNTKEMIALTKGP
                     APYYLGTSSPKVLIVEYGDFNCSHCRVVSPEIRKAVNQNKDIVQYIWRDWPGQDNSEL
                     LAQAARCAGEQAGSLGFWLFHDQLLLNQSQFTTTSQLMELAEEYSLSPTRLQDCIEQQ
                     RTLGYLQKDYQDAIALGGIGGTPTWFINGYRFSGELPADFFNYMIKELTKEK"
     gene            2840..3601
                     /locus_tag="PKV49_02885"
     CDS             2840..3601
                     /locus_tag="PKV49_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPL22588.1"
                     /translation="MKIITWNINGWRAVWHKGLLDFIINKQPDLLGLQETKINDHLAS
                     NWSDKIPGYKIFWHGAERPGYSGTAVIAQEDLAVNLTNGLNDKQFDLEGRCQISELAD
                     FYWLNIYFPNANRQLSRLPYKQAFNQAVRSLVTKLEQSKPVIISGDFNVAHQPIDLAN
                     PKANEGSAGYTLIERQFIDQLLADGWIDIWRQLYPNKQQYTWWTYRFGARARNIGWRI
                     DYFLISPSLLAKIKDVQILDQVTGSDHCPVELIIT"
     gene            3605..6124
                     /gene="leuS"
                     /locus_tag="PKV49_02890"
     CDS             3605..6124
                     /gene="leuS"
                     /locus_tag="PKV49_02890"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPL22589.1"
                     /translation="MSEKYQHHDFEARWEKLFQADGLYVSAIDHNRPKLYILDMFPYP
                     SGAGLHVGHPKGYIATDIVSRYQRMRGYNVLHPIGWDAFGLPAENYALKNKVHPSVAT
                     ANNIATFKRQLGILGLSYDWQREINTTDPQYYRWTQWIFLKFYNSFYDSEQKKARPIE
                     QLVIPDGLSFEERRQFVDNQRLAYESNEPINWCPSCQTGLANEDLENGLCERCGSPVE
                     LKPIRQWVLRITKYADRLLDDLAILSDWEDSIKDMQRDWIGRSQGALVTFTVVDDKSN
                     TIAELPVFTTRPDTLFGCSYVAICPEQALLPQLQPILTNWSEVKKYIDLAKNKTDLDR
                     TDFNKDKTGVRLEGCQAINPVNGQPVPIFVADYVLGGYGSGAIMAVPAHDERDFAFAQ
                     KYNLPIKQVIASSNNDQLPYTGDGRLINSQFLDKLDVASAAARIIDWLTSRGLGRPQT
                     NYRLKDWTFSRQRYWGEPIPMVHCQQCGTVAIPENQLPVTLPQVDHYEPSGTGESPLV
                     NIKQWLTVACPICGQPAQREANTMPQWAGSSWYYLRYIDPTNQQVLVDKDVEKYWSPV
                     DLYVGGAEHATRHLIYARFWHKFLYDLGIVNHPEPFIKLRHVGLIMGEDSRKMSKRWN
                     NVVNPDDMVKQFGADALRLYEMFMGPFDQSCAWNTNGIIGTRKFLDKVWSLQARLVDQ
                     PDSEAVKKLLAKTIIKVGQDIEQFKFNTAVSALMILVNKLFEQPGISRDSYQQLLIIL
                     SPLAPYISQEIWYKLGNNNYISQQPWPVINNQEISDKVSTIVVQFNGKTRGTIEVSTS
                     ADQTAVIDELKQQAALFKYWPAGQEPQKIIFLPGRLINLVF"
     gene            6127..7032
                     /gene="rsmA"
                     /locus_tag="PKV49_02895"
     CDS             6127..7032
                     /gene="rsmA"
                     /locus_tag="PKV49_02895"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPL22590.1"
                     /translation="MSVITAFNSSTALRRETEQLVNLYGAEPKHSRGQNFLIQPKTYQ
                     IIIEAANLQASDEVLEIGPGWGFLTMALAEMVKKVVSVELDEQLSEILPSRFAAAGFD
                     NIELVNHDILKTVINRPDLQDNDKLSNINLAANYKLIANLPYNITSICLRRFLTGEVD
                     KPALMVLMVQKEVAERLTAKPGQLSLLGLLAQYYSQIEYRSLVPAVNFWPQPKVDSAI
                     IRLTPIKTILSANDEKLFWRLARFGFSARRKMLKNNLAAGLKINERIISQALEKIGQK
                     ANCRAQDLTVEQWIKLVALLRPVML"
     gene            7074..7754
                     /locus_tag="PKV49_02900"
     CDS             7074..7754
                     /locus_tag="PKV49_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22591.1"
                     /translation="MTHINPDNQQPMSPEEFAMWKASRRAQRQKNWALAGLSTMSVAI
                     VVMWFLQFKQNITAPLYRQMGFTNNKEIETAFKGKTAEQLAQQDEALKLADTDKDGLS
                     DYDELNLYNTSPFLEDSDSDGVLDKVEIERGQDPNCPIGKDCSGSILVNPVATPTGSD
                     AVNIGTTIDSPIGNSVTGSSSLAATALPSDATSLRQVLLESGMDKTMLDQFSDQELLE
                     AYQEALKQ"
     gene            7758..>8261
                     /locus_tag="PKV49_02905"
     CDS             7758..>8261
                     /locus_tag="PKV49_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22592.1"
                     /translation="MKMSFRLFWGGLVLVYLLFGVNSYTKAQEADTMTPPPVISPPVA
                     GAPVTDTTSWLDRTADKISSYITNQMWAAFKKATTRTYSSLLRKYANEFANQTALYVA
                     SGGKGQKAEFYTYTWSTFATDVARNAAGTFVENYFTALEEGSTKTGTKTQDVSCGTKT
                     VHLVLTAT"
BASE COUNT         2354 a         1631 c         1782 g         2494 t
ORIGIN      
        1 ccaataatct gtatgccata tttaaatagc gatcccatta gttttgctat tggcatagtt
       61 gaattttctg taaaaacata ctcagacata aaaccaggta gaccaacctg gggagtaaac
      121 tttaaagccc cagatgattc attgctacta gtagtaactg atgcggccaa aacactatgg
      181 ccaataagca ataacgccac aattaaaatt atactgccgg ttattaacgt cttatatttt
      241 ctggctagac aattgcccat atatttttaa ttgttttgtt tattttgatt aaatcaatat
      301 caccgcccaa accgatgatt tggctggaaa tattgttttt atttaatggt ttgattgtca
      361 taatatcgct aaatagcggc ggctgagctc caagtccggg cgagcggtca attactatga
      421 tttgttcggc ttgatttaaa tagcgctcaa taactttctc agggaatgga cggtataatt
      481 taatctttaa aattgccact gaacccggtt tttgtttatt agccttatca gctaagtcct
      541 tgagctcacc aatgattgag cccatcgcta caataactag tttagcttga gccggaccat
      601 aatattcaac tgccggatcg ctactatgat tattgcgtcc gagcagtttt gtttttattg
      661 gttgccacag attattaaat tgctggttgg tattcttaat aattggccaa ctattggcaa
      721 taactcgcct taactccagt ctttgttgtt gatattcagt tggcatagca aaccaaccgg
      781 tagtttgtgg ttttttagta tttaaaacag cttttatctt attatttaat agttttttaa
      841 ctaattgttt atttggtaaa gccaccggtt cactggcgtg ggttaggcgg aagccgtcaa
      901 cattaaccat gaccggtaat cttaattttt gacttagggc aaaagccaac caatgttgtt
      961 caatcgcttc ttgattatct tcagcaaaga acattagcca gccggcttcg cgcaccgcca
     1021 tggcgtcttg gtgatcattc cagatattga ttggtgccga aattgaacga ttggcacagg
     1081 tcataactag tggcaagcgc aaaccggcta aattataaat aacctcagtc atatacagca
     1141 gtccttgcga gctggtggcc gtgtaggcag ccacacccat ggcagcggcg cctaaggcga
     1201 ttgatagggc tgaatattcg ctctcagctt ggacaaaaga aaaatcagct tgaccgctgg
     1261 ctgataattt gactagctgt tcaataattc cggtctgtgg ggtaatcggg taggcagcca
     1321 ctactccagg ctgacaaaga gccaccattt tggcaatagc ctcagaacct ttcagaaagg
     1381 ttaattgtcc ggcgctatca ttgctagtta ttggtttcat gccgatttaa ttaatttaat
     1441 cgctaattga cgattggcca aaaaagcttg ctgatttttt tgagcgatta ttggattggc
     1501 aaattgttcc gacagggcct taaaccaatt atcttcatca atcagcgacc aagcagcact
     1561 taaaccgccc aataggccaa ttgctaaatc ttgattaaaa atatctagag aaatttggcg
     1621 gctatcataa gtcagtagtt tagtttttgg ttgatagtta gccaaatcgc tatggttaat
     1681 aattgttaat tgtttaccta aatcattttg ttgataaagt cgataatcat taattattag
     1741 aatgtcgcag cgattaattt tttggcgatt acggattgat tggtcagcta tcctaacata
     1801 ggcctcgacc ggtgtgccgg ttcgctctac tccaaaaaat ggcattgatt gcgaccagta
     1861 accttggtga aaagcggccc gagctaacgt ggcggccgtt gtgactacgc cttgtccacc
     1921 cagaccgagt attttaatag taattgatct atttaatttt ttagttaggg ctgccattat
     1981 ttttccttgg ttaattcttt aatcatataa ttaaaaaaat cagccggtaa ttcgccgcta
     2041 aaacgataac cattaataaa ccaggttgga gtgccgccga taccgcccaa agcgatagcg
     2101 tcttgatagt ctttttgtaa ataacccaga gtgcgctgtt gttcaataca atcttgcaat
     2161 ctggtaggtg aaagactgta ttcttccgct aattccatta gttgactggt agtggtaaat
     2221 tgactttgat tgagtaaaag ctgatcatga aatagccaga agccgagact gccagcttgt
     2281 tcgccagcgc aacgagcggc ctgagccagt aattctgaat tgtcttggcc tggccaatcg
     2341 cgccagatat attgaacaat atctttattt tggttgacgg ctttgcgaat ttctggcgag
     2401 accactcgac aatgtgagca attaaaatcg ccatattcaa caattagcac cttgggactg
     2461 gatgtcccca aataatatgg agccggccct ttagtcagag caatcatttc tttagtgttc
     2521 aatcctggat tgctttgttg caacattaat tccgcggcgg ttattcggcc ggtttttaac
     2581 tgccaaacca ggataacaac cttgtaaaga taaacagcta cgaataagct aaatagagcg
     2641 gctataatta aaattattat accccaccaa cggcgatacc agcgtcttgg ccgacggccg
     2701 ctggatctta atagcgcatt aaagcgattt tgccagccgg acgacccctc cgttattggt
     2761 aatggtttgc ttttataatt ttttttcatt gcttgattaa ttagtttggg ctataatatt
     2821 ataacaaatt tatcaactaa tgaaaatcat tacttggaat atcaatggct ggcgagcagt
     2881 ttggcataag ggcctgcttg attttattat caataagcaa ccggatttgc ttggtttgca
     2941 agaaaccaag attaacgacc acttagctag caattggtca gacaagattc ctggctataa
     3001 aatattttgg catggcgcgg agcggcccgg ctatagcggc acggccgtta ttgctcaaga
     3061 ggatttagca gttaatttaa ccaatggcct taatgataag caatttgacc ttgaaggccg
     3121 ttgtcagatt agcgaattag ctgattttta ttggttaaat atttattttc ccaacgccaa
     3181 tcgccaactt agccgtttgc cctataaaca ggcttttaat caagcagttc ggtcgctagt
     3241 tacaaagctg gagcaaagca agccggttat tattagcggt gattttaatg tcgcccatca
     3301 accgattgat ctggccaatc ccaaagccaa tgaaggcagc gccggctata ctctaattga
     3361 aaggcaattt attgatcaat tattggcgga tggttggatt gatatttggc gtcaacttta
     3421 tcctaataag cagcaatata cttggtggac atatcgtttt ggcgcccgcg ctcgcaatat
     3481 tggctggcgg attgactact tcttgattag cccgagttta ttggctaaaa taaaagatgt
     3541 gcaaatattg gatcaagtta ctggatcgga tcattgtcca gttgaattaa tcattaccta
     3601 gaatatgtca gaaaaatatc aacaccacga ctttgaagct cgctgggaga aattgtttca
     3661 agctgatggc ctttatgttt ccgccattga tcacaatcgc cccaagcttt acatcttaga
     3721 catgtttcct tatccgtccg gagccgggct gcatgttggc catcccaagg ggtatattgc
     3781 taccgatatc gtgtctcgct atcaacgaat gcgcggttat aatgttttgc acccgatagg
     3841 ttgggatgct ttcggtttac cggccgagaa ttatgcccta aaaaataaag ttcatccgag
     3901 cgtggccact gctaacaata tcgccacctt taagcggcag ctgggcattt tgggtttgtc
     3961 ttacgattgg caacgggaaa tcaatactac tgatccgcag tattaccgct ggacgcagtg
     4021 gatatttctc aaattctata attcctttta tgattctgag caaaaaaagg ctcggccaat
     4081 tgaacagctg gttattcctg atgggcttag ttttgaagag cgacgccagt ttgttgataa
     4141 tcagcgtttg gcctatgaat ccaatgaacc gatcaattgg tgtccatcgt gccagaccgg
     4201 tttagctaat gaagatttag aaaacggtct ttgcgagcgt tgcggtagtc cggttgagct
     4261 taagccgatt cgtcaatggg tgttaagaat caccaaatat gccgatcgct tgcttgacga
     4321 cttagctatt ttatcagatt gggaagattc tattaaagat atgcaacgcg attggattgg
     4381 ccgtagtcaa ggggcattag ttacttttac ggtagttgac gacaaaagta acactatcgc
     4441 tgagttgccg gtttttacta cgcgcccgga tactttattc ggttgtagct atgtggctat
     4501 ctgtcccgaa caagcattat tgccgcagct acagcccatt ttaaccaatt ggtccgaggt
     4561 taaaaaatac attgatttag ccaagaacaa aactgattta gatagaactg attttaataa
     4621 agataaaacc ggtgtgcggt tagagggctg tcaagccatt aatccggtca atggtcagcc
     4681 agtgccgatt tttgttgctg attatgtctt aggcggttat ggcagcggtg ccattatggc
     4741 tgtcccagct catgatgagc gagattttgc ctttgctcaa aaatacaact tgccgattaa
     4801 gcaagttatc gcttcatcaa ataacgatca attaccatat actggtgatg gtcggcttat
     4861 taattctcaa tttttagaca agcttgacgt tgcttcagcc gctgcccgga ttattgattg
     4921 gctaactagt cggggattgg gccgaccgca gactaattat cggctcaaag attggacttt
     4981 ttcgcggcaa cgctattggg gtgaaccgat tccgatggtt cattgccaac aatgtggcac
     5041 ggtggcgata ccagagaatc agttgccggt aaccttacct caagttgatc actatgagcc
     5101 gagcggaacc ggcgagtcgc cattggtcaa tattaaacaa tggctaaccg tagcttgtcc
     5161 gatttgtggt caaccagctc aacgagaagc taatactatg cctcaatggg ctgggtcatc
     5221 ttggtattat ctgcgctata ttgatccgac taatcaacag gttttagttg ataaggatgt
     5281 agaaaaatat tggtcgccgg ttgatttata tgtcggcggc gctgaacacg ccacgcgcca
     5341 cttaatttat gctcgattct ggcataaatt tctttatgat ttaggaattg ttaatcatcc
     5401 cgagccattt attaagttgc gtcatgttgg cctaattatg ggtgaagata gccgtaaaat
     5461 gagtaagcgt tggaataatg tggtcaatcc tgacgatatg gttaagcagt ttggtgccga
     5521 tgcgttacgt ttatatgaaa tgtttatggg cccgtttgat caatcttgcg cttggaatac
     5581 taatggcata atcggcacgc gcaaatttct ggataaagtt tggtcactgc aggctagact
     5641 ggttgatcaa ccagattctg aggcagtaaa aaaactatta gctaaaacaa ttattaaagt
     5701 cggtcaagat attgagcagt ttaagtttaa tacagcggtc agcgcgctaa tgattttagt
     5761 gaataaatta tttgaacaac caggaatatc tagagatagt tatcagcagc tcttaattat
     5821 tttatcgccg ctcgctcctt atattagcca ggaaatttgg tataaactag gtaataataa
     5881 ttatattagt cagcagccat ggccagttat caataatcaa gaaatttctg acaaggtgtc
     5941 gacaattgtt gttcagttta atggtaaaac tcgcggaact attgaagtat caacatcagc
     6001 cgatcaaacg gcggttattg acgaactaaa acaacaagct gcgctattta aatattggcc
     6061 agccggtcaa gagccgcaaa aaatcatctt tttgcctggt cgcttgatta atctggtttt
     6121 ttaattatgt cagttattac cgcatttaat tcttcaaccg ctctacgccg cgaaactgaa
     6181 caattggtta atttatatgg tgccgaacca aagcatagcc gtggtcaaaa ctttttaatt
     6241 caacccaaga cttatcaaat aattattgaa gccgctaatt tacaagccag cgatgaagtc
     6301 ttggaaatcg gtcctggctg gggtttttta actatggctt tagctgagat ggttaaaaaa
     6361 gttgttagcg ttgagctcga tgagcaatta tcagaaatac tgcctagccg ctttgccgcg
     6421 gctggttttg ataatattga gctagttaat catgatattt taaaaacagt catcaatcgt
     6481 ccagatcttc aggataacga taaattgtcc aatattaact tggctgctaa ctataaattg
     6541 atagctaatt taccatacaa tataacctca atctgcctgc gacgtttttt aacgggcgaa
     6601 gttgataagc cagctttaat ggtgttaatg gttcaaaagg aggtggcgga gcgcttgact
     6661 gccaagcctg gtcaattaag tctgcttggt ttgttagccc agtattacag tcagattgaa
     6721 taccgatcat tagtaccagc tgttaatttt tggccccagc caaaagttga tagcgctatt
     6781 atccgcttga cgcccattaa aacgattttg tccgctaacg atgaaaaact tttttggcga
     6841 ctagctagat ttggttttag cgcaagacga aaaatgctta aaaataattt ggcagccggg
     6901 ctaaaaatta atgaacggat aattagtcag gccttagaga aaattggcca aaaagcaaat
     6961 tgtcgcgccc aagatttaac agttgagcag tggattaaat tagttgccct gctacggccg
     7021 gttatgctat aattgaaacg aacaattttt taatttggtt tagtaattta tttatgaccc
     7081 atatcaatcc ggacaatcaa caaccaatgt ccccggaaga atttgcgatg tggaaagcca
     7141 gccgccgagc tcaacggcaa aaaaattggg cactagctgg acttagcacc atgtcggtag
     7201 ctattgtggt aatgtggttt ttgcaattta aacaaaacat aaccgcacca ctctatcgac
     7261 aaatgggatt tactaataac aaagaaattg aaaccgcctt taaaggcaaa acggctgagc
     7321 agctggctca acaagatgag gctcttaaat tagccgatac tgataaagac ggtttaagtg
     7381 attatgatga gttaaatctt tataatactt cgccattttt agaggacagt gatagtgatg
     7441 gcgttttgga taaggtagaa attgaacgcg gtcaagaccc taattgtccg attggcaagg
     7501 attgtagcgg gtcaatttta gtcaacccgg tggcgacccc aaccggttcc gatgcagtta
     7561 atattggtac caccatcgat tcgccaattg gtaatagcgt tactgggtct tctagtttgg
     7621 cagccaccgc attgccgtct gatgctacta gtttaagaca ggttctctta gaatcaggta
     7681 tggataaaac aatgttagat caatttagcg accaggaatt actggaagct tatcaggaag
     7741 ctcttaaaca atagcttatg aaaatgtctt ttcgtttgtt ttggggcggt ttagtattag
     7801 tttatttatt gtttggtgta aacagttata ctaaagcgca agaggctgat acgatgacac
     7861 ccccaccggt tatatcgcct ccagttgccg gtgcgccggt tactgatact acttcttggc
     7921 tggatagaac agccgataag attagtagtt atattacaaa ccaaatgtgg gcagctttta
     7981 agaaggcgac taccaggaca tatagctctc tattgcgaaa atacgctaat gaatttgcca
     8041 atcaaacagc actctatgtg gctagtggtg gcaaggggca aaaagctgaa ttttatacct
     8101 atacttggtc aacatttgct actgatgtag ccagaaatgc tgccggaaca tttgtggaaa
     8161 attattttac cgccctagag gaaggttcaa ctaaaaccgg cacaaaaaca caggatgttt
     8221 cttgtggtac taaaactgtt catttggtat taacagcaac a
//
LOCUS       DAOPAI010000022         8099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_8179_length_8099_cov_5.358652, whole genome shotgun sequence.
ACCESSION   DAOPAI010000022 DAOPAI010000000
VERSION     DAOPAI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_8099_cov_5.358652"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1041)
                     /locus_tag="PKV49_02910"
     CDS             complement(109..1041)
                     /locus_tag="PKV49_02910"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPL22593.1"
                     /translation="MICTKCNRQMADNSTVCPNCGQPVIAPEQIIREVKVRRLQRYLF
                     YAIVFMVVIAAVAIMVRIYNANTKLLLEISQVKQGLEGAQGELTATQAELAQKKKDLA
                     KIQAEFEANSQKMQAADNQLKQKTAAYQALLDEKTKIEQGHEQCRMDLNLADANIYGL
                     IIKLGIGVTNKNLASIPLADANLGGQDSDNDGLSDTIEKSLGTDPNKADSDNDGFDDK
                     LEAVTGYNPLGKGMLPINPKYVESVKGKILLQMEGDKSAWYVAGDGKRYFLGNPGDAY
                     RVMRQNEYWTKDWPGQATPLIGTSSSSTVVEVVE"
     gene            1232..2596
                     /locus_tag="PKV49_02915"
     CDS             1232..2596
                     /locus_tag="PKV49_02915"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPL22594.1"
                     /translation="MTTKSKLRQLILLLGDWLLLYASLAAALLIRYRHWPEPDLWQQH
                     TAIFNWLFIVWLLIFYVANLYNLNILAKGPRFTERVIKAIVPAGLLSLIIFYLLPAVT
                     IAPKTNLLIFSLIYTGSFLAWRHLWRHSLRKTLPRNNVLLIGYNQMADELWQLLNNAP
                     HLGYKPRLLFTRNDDQSPVGWTVIRQADQLAAAIKDYHINTLVIVDHYRADEQINQIL
                     FSLLPLNLTYLTLSSFYEQLTGKVPLAAIGQLWFLENLNLTEKRWYLTAKRGFDVVVA
                     TIGLIITAPLWPLIALIIKISSRGPIIFQQQRLGRNNQSFTIYKFRTMKITGNDFQPT
                     GDNDQRITAGGRWLRQTRLDEIPQLINIIKGDMSFIGPRPERPELIANLTKELPFYHV
                     RGLIRPGITGWDQVSGEYHSPSLEDTYKKLQHDLFYLKNCSIYLDLTIILKTLTTVLS
                     REGK"
     gene            2601..3716
                     /locus_tag="PKV49_02920"
     CDS             2601..3716
                     /locus_tag="PKV49_02920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22595.1"
                     /translation="MAKKILQINKFYYPWIGGVETVVKDIADSLNGQPDWQVDVLACQ
                     AKGRRTIENINGATVYRAASYGKLLGMPVSLDFFRLLKKLWNNYDQIIIHHPFPLVNL
                     ALPFLPKKPLVIWYHCDIVRQKISKLPFLPFINYGLRQARLILASSQRLIDFSKPLGR
                     YKNKCRVVYFGLKLADYQVDETINKQAQTIRQQYAVNDKLILSIGRLVYYKGFEYLIA
                     AMADNDYGLLIVGQGPEEAKLRQLINHYNLNERVQIIPAVADTRPYYAASDLFVLPSC
                     AASEAFGIVQLEAMAYGKPVVNTDLPTGVPEISLDGQTGLTVPPQDSAALATAIKKIL
                     DNPELAEGYGQAAGQRARAIFDKQLFTEKLNQLLSDQ"
     gene            complement(3699..4841)
                     /locus_tag="PKV49_02925"
     CDS             complement(3699..4841)
                     /locus_tag="PKV49_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPL22596.1"
                     /translation="MKDYRGYFLRLVATLIDAFGWLLFIGLSRPAVQPNQVKRLLVLK
                     FDRIGDAFLALPTIEALRQLFPAAEITIACSPWNKAVLANNPAIDRLLSLESLPDVHH
                     SRLTDFLRLTKIKQLASFIKVQETEVVVDLQGSPMNVLAMFWSGSKHRLGYRPKVFSF
                     LLTQAASYQRQQPQTEVYFSLAKLLGYTGAMPTAAIQLSTNDLATVNNFSQQQQLTDF
                     IILHLGAGRSYRQWPLANFVALTQRIIDNYPGYKLVVIGGAEDQLLFDKLAAALSATD
                     RLVNAIGQLNIPASYQLIGQAKLFIGNESGPGHLAAAQGVPTISFMNPWSGINRWQAR
                     GPQVNLFYKSAHYCQGIRCHLEPCPNMLAIAVDEVWATVERLLVRQ"
     gene            complement(4846..5601)
                     /locus_tag="PKV49_02930"
     CDS             complement(4846..5601)
                     /locus_tag="PKV49_02930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPL22597.1"
                     /translation="MNYQKLRRQSFFQLQEPNFSEILAALSTDKKYDFLRNQATQISY
                     TQFCFYARNFCRAYFNTEKIQVLDWGCGHGQNSVLLDDLGFDVQAGDVIECQSDRPLL
                     AIKSIDFTAFNHQSRLPFDDQSFDAVFSFGVLEHVPDEIASLKEINRILRPDGLFFCF
                     NLPYFYSWGQRLAHLRGNYYHDRLYQEKYVKSTLPKYNYQLLDLWHLGFFPKVVVNYG
                     RFSSSLEKLDQFLTNYTPLRYLATSLNFVARKK"
     gene            complement(5609..6598)
                     /locus_tag="PKV49_02935"
     CDS             complement(5609..6598)
                     /locus_tag="PKV49_02935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPL22598.1"
                     /translation="MPSSKIYFIILNFNGYSDTVECLESLRRLKNQDFQVILVDNAST
                     NDEGKQLAAEFPEYIHILNEKNTGFAGGNNIGVARALQEADCGYIFFLNNDTTVEPDL
                     LDQLLVVVDNPENQAYGFFQPKMINYFQPELMDSAGLDYSRNGLGFNRGGNKPVTDYQ
                     KNQEILGCCGGAFLCRRQAVEDLWRQAGDFFAEEFFAYCEDLDASLRLQWAGWSALFV
                     ADAIVYHKGGQTANKFSARKVFWPNRNSYWVIIRNWPLIDILKNGHWLFLGQIGMVVN
                     AFLRRGKLGWWAVKGKIEAGQSSIKMWHRRAAIQTKRSNWLRIKKLMILKWRP"
     gene            complement(6606..7904)
                     /locus_tag="PKV49_02940"
     CDS             complement(6606..7904)
                     /locus_tag="PKV49_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPL22599.1"
                     /translation="MVDLLAIKMIRFKNLAKLLKRGITNKYSKGIGWLFVGQFVSLFF
                     SFAVGLTVARYLGPEEYGILSYALSFAGIFTFIASLGADGIISRELAKNDGDESEVLG
                     TGLLLKIAGSIFASLLCCLILFLVPDKQGYRYFVLWSIPVILFQFSNIFNLWFQARIL
                     SKWISLSQIGSIVLLAIIKVSLVVFDAPVRFLILAYSLDIVFWAMFGYCFYVLHGGLL
                     NWRLSLKMLFNILKESWLLMLSSASCILLFKIDQVMVGQMLGKLASGYYAVAVKLSEV
                     WYFLPVLISNSVLPALINGQKNSWLVYRRRKFWLLMLLLSFSLLFSIFMLFFAKQLIG
                     LLFGNQFLPATPIAKIYAWSSIGMFLGTGLWVSLVSEAKNKIIFMAYLSALILNIYLN
                     VILIPIIGLSGAAWATVISYSLVPMILLLPGLSHKKIFNQ"
     gene            complement(7867..>8099)
                     /locus_tag="PKV49_02945"
     CDS             complement(7867..>8099)
                     /locus_tag="PKV49_02945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22600.1"
                     /translation="IGAEGLDLVDGQDVLIYRSQDEFVKKLSRLLSSSDLWKNLVSAA
                     RTKVESAYDWRVIVKNSLAIYGRLIGNQNDKI"
BASE COUNT         2587 a         1826 c         1531 g         2155 t
ORIGIN      
        1 caatgctccc cccttaaaca ttgatcttaa gccaatctag ccgggccgcc taacaaaaac
       61 aacccgcgga cgcagattgt ttgccccctc cttggatatt atttaatttt attctaccac
      121 ctcaacaact gttgaactgc tgctagtgcc aatcagcggt gtagcttgac cgggccaatc
      181 tttggtccaa tattcatttt gacgcataac tcggtaagca tcaccgggat tacctagaaa
      241 ataccgcttg ccgtcgccag caacatacca agctgattta tcaccctcca tttgtaataa
      301 aatcttgccc ttaactgact caacatattt tggattaatc ggcaacatac ccttgcctaa
      361 aggattgtag ccggtaacag cttcaagctt gtcatcaaaa ccatcattat ccgaatcagc
      421 tttgttggga tcggtaccca gagacttttc aatcgtatca gatagaccat cgttatcaga
      481 atcttgaccg cctagattag catccgctaa aggaatactg gctaaattct tgttagtcac
      541 gccaatgcct agtttaataa tcaatccata aatattggca tcagctaaat tcaaatccat
      601 ccggcattgt tcatggcctt gttctatttt ggttttttca tctaataatg cctgataggc
      661 agccgttttc tgtttaagct gattgtcggc cgcctgcatt ttttgactat tagcttcgaa
      721 ctcggcttgg atcttagcta gatccttttt cttttgagcc aattcggctt gagtagccgt
      781 caattcaccc tgagcgcctt ccagaccctg cttaacttgg ctaatctcta acaataattt
      841 ggtgttggca ttataaatcc gcaccataat agcgacagcg gcaataacta ccataaacac
      901 tatcgcataa aacagatagc gctgtaggcg gcgcactttg acttctctaa taatctgctc
      961 gggggcaatg accggctgac cgcaattggg acagacggtg ctattatcag ccatttggcg
     1021 attgcacttg gtgcaaatca taagtgaggg gttaagctag cttactaaag ccattataat
     1081 acagactggc aacttgggca aataattttt attggctaaa aataaatact tttttaaatt
     1141 agccagttag gtctctgttt cttaatcttt taggccaaaa ctagataaaa ttagcttgct
     1201 tttttgtggg cggcgattat aataaaatca tatgaccaca aaaagtaaat tacggcaatt
     1261 gatattatta ctgggcgact ggttactgct ttacgctagt ttggcggcgg cgctattaat
     1321 ccgctatcgg cattggccgg aacccgatct ttggcagcaa cataccgcta tctttaattg
     1381 gctgtttatt gtctggctgc taattttcta tgtagccaat ctttataatc ttaatattct
     1441 agctaagggg ccgcgtttta ccgaacgagt cattaaggcc atcgtgccag ccggcttatt
     1501 gtcattaatt attttttatc tactgccggc ggtaactatc gcgcccaaaa ccaatctatt
     1561 aatttttagc ttaatttata ccggctcgtt tctggcttgg cgacatctct ggcgccatag
     1621 tttacgcaaa actttgccgc gcaataatgt tctcctgatt ggctataacc aaatggccga
     1681 tgaattgtgg caattattaa ataatgcacc gcatcttgga tataagccaa ggctattgtt
     1741 tacccggaat gacgaccaat cgccagttgg ctggacagtt atccgtcaag ctgaccaact
     1801 ggctgcggcg attaaagatt accatatcaa tacattagtt atcgtcgatc attatcgcgc
     1861 tgatgagcaa attaatcaaa ttctttttag cttattacct ctcaatctaa cttatttgac
     1921 tttatctagt ttttacgaac aattgaccgg taaagtgcct ttggccgcta ttggccaatt
     1981 atggttttta gaaaatctta atttaacgga aaagcgttgg tatttaaccg ctaaacgcgg
     2041 ctttgatgtt gttgtggcaa ccattgggct aattatcacc gcgccgctat ggccgctaat
     2101 tgccctgata attaaaatta gcagccgcgg accgattatc tttcaacaac aacggctagg
     2161 ccgcaacaat caatctttta ctatttataa attccgcacg atgaaaatca ccggcaatga
     2221 cttccagcca accggcgata atgaccagcg cattacggct ggcggccgct ggctaagaca
     2281 aaccaggttg gatgaaattc cgcaattaat caatattatc aagggcgata tgagttttat
     2341 cggtcctcgt ccggaacggc cggaattaat cgccaatctg actaaagaat tgccattcta
     2401 tcacgtccgc ggtttaatcc gtcccggtat aaccggttgg gaccaggtgt cgggcgaata
     2461 tcattcacca tcactagaag atacctataa aaaactgcag cacgatctct tttatctaaa
     2521 aaattgttca atctatttgg atttgacgat tattttaaaa acgctaacga cggttttatc
     2581 tagagaagga aaatagaatt atggctaaaa aaatcttaca aattaacaag ttctactacc
     2641 cttggatagg cggtgtggaa accgtagtga aagatattgc cgacagcctc aatggccaac
     2701 cggattggca agtggatgtt ttggcttgcc aagccaaagg acgacgaaca atagaaaata
     2761 tcaacggcgc tactgtctac cgggcggcca gctatggcaa attgctaggt atgcccgtat
     2821 cgctagattt ttttcgtttg ctgaaaaagc tatggaataa ttatgatcaa ataatcatcc
     2881 atcatccttt tccgttagta aatctagctc taccgtttct gccgaaaaaa ccgctagtga
     2941 tttggtatca ctgcgatatt gttcggcaaa aaatcagcaa gttgccgttt ctcccattta
     3001 tcaattacgg attgcgtcaa gctagactta ttttagccag cagccaaaga ttgattgatt
     3061 tttccaagcc actaggccgc tacaaaaata aatgccgcgt ggtttatttt ggtcttaaat
     3121 tagctgatta tcaagtggat gaaaccatta ataaacaagc gcaaacaatt cgccagcaat
     3181 atgccgttaa cgataaatta atcttgagca ttggtcgact ggtttattat aagggctttg
     3241 agtatctaat tgcggccatg gcagataatg attatggcct actaatcgtc ggccaaggcc
     3301 cggaagaggc caagttgcgc caattgatta accactacaa tctaaacgaa cgagttcaaa
     3361 taatccccgc cgtagccgat acccgcccct attacgcggc tagcgatcta tttgtcctgc
     3421 cgtcttgcgc ggctagcgaa gcttttggca tcgtccagct ggaggccatg gcttatggta
     3481 agccggttgt caatactgat ctgccaaccg gcgtgccaga aatcagcctg gatggccaga
     3541 ccggcttgac agtgccgccg caagattcag cggctttagc gacagcgatt aaaaaaatat
     3601 tggataaccc cgagctagct gaaggatacg gccaagcggc cggtcaacga gcccgagcaa
     3661 tatttgacaa gcaattattt actgagaaat taaaccaatt attgtcggac caataaccgc
     3721 tccaccgtcg cccaaacttc atcaacagca atagctagca tattgggaca gggctctaaa
     3781 tgacaacgaa ttccctgaca atagtgggct gatttataaa acaaatttac ttgagggccg
     3841 cgcgcttgcc aacgattgat accggaccat ggattcatga aagagatagt tggcacgcct
     3901 tgagcggccg ccaaatggcc aggaccggat tcattaccga taaacaattt ggcttgacca
     3961 attagctggt agctggccgg gatattgagc tgaccaatag cgttaactaa acgatcagtg
     4021 gccgacaaag cggcggccag tttatcaaat aataactgat cttccgcgcc gccaataacc
     4081 actagtttat agccgggata attatcaata atccgctgag tcagggcaac aaaattagcc
     4141 agcggccatt gccgatagct acggccggcg cccaagtgaa gaataataaa atcggtcaac
     4201 tgctgttgtt ggctaaaatt attaactgtg gctaaatcat tagttgacag ttgaatagcc
     4261 gccgttggca tcgctccagt ataacctagt agcttggcta aagaaaaata aacctcggtc
     4321 tgcggctgct gtcgctgata gctggcggct tgagtcagca gaaaactaaa aactttgggc
     4381 cgataaccta aacgatgctt agaacctgac cagaacatgg ccaaaacatt catcggactg
     4441 ccctgcaaat caaccacgac ttcggtttcc tggactttaa taaagctagc cagctgctta
     4501 atcttggtca gacgtaagaa atcagttaag cggctgtgat gaacatcggg caaagattca
     4561 agagacaaaa gtcggtcaat ggccggatta ttagctaaaa ccgccttatt ccaaggagaa
     4621 caagcaatag ttatttcagc agctggaaag agctggcgca aagcctcaat ggttggcaga
     4681 gccaaaaaag catcaccaat ccgatcaaat ttcaaaacca atagccgctt aacctgattc
     4741 ggctggacag ccggccgaga cagcccgata aatagtagcc aaccaaaggc gtcaatcaag
     4801 gtcgccacta agcgtaaaaa ataaccgcgg tagtctttca taaaattatt tctttctggc
     4861 tacaaaatta agactggtgg ccagatagcg caatggagta taattagtca aaaactgatc
     4921 aagcttctct agagaagaac taaatcgacc ataattaaca accactttag gaaaaaatcc
     4981 caaatgccac aaatctaata actgataatt atatttaggc agagtgctct tgacatactt
     5041 ctcctgatag agccgatcat gataataatt gcctcgtaga tgggctaatc tttgccccca
     5101 agaataaaag tagggcaggt tgaaacaaaa gaacagacca tcgggacgta aaatgcgatt
     5161 aatttctttg agcgaggcta tttcatcggg cacgtgttct aaaactccaa aactaaaaac
     5221 agcatcaaaa gactggtcgt caaatggtaa ccttgactga tgattaaaag cagtaaaatc
     5281 tattgatttg atagccaaaa gcggacgatc agattgacat tcaataacat caccagcttg
     5341 aacatcaaac cccaaatcgt ctaacaaaac tgaattctga ccatgaccgc aaccccagtc
     5401 taaaacttga attttttcgg tattaaaata tgctcgacaa aaatttctag cataaaaaca
     5461 aaactgcgta tagctaattt gagtcgcctg atttcttaaa aaatcatatt ttttatcagt
     5521 agacaaagca gctaaaatct cagagaaatt aggctcctgc aattgaaaaa aactttgccg
     5581 gcgtaatttt tgataattca taaaatagtt atggacgcca ctttaaaatc attaattttt
     5641 tgattcttaa ccaatttgat cgcttagtct gaatagctgc tcgacgatgc cacattttaa
     5701 tggagctctg accagcctct attttgcctt tcaccgccca ccagcccaat ttaccgcggc
     5761 gcagaaaagc attaacgacc atgccaattt gacccaaaaa taaccagtgg ccattcttca
     5821 gtatgtcaat taatggccaa tttctaataa tcacccaata actgttgcga ttaggccaaa
     5881 aaaccttgcg agcactaaac ttattggcgg tctgaccacc cttgtgataa acaatggcat
     5941 cagccacaaa taaagctgac caaccagccc attgtaagcg caaagaagca tctaaatcct
     6001 cgcaataagc aaaaaactct tcagcaaaaa aatcaccggc ctgacgccat aaatcttcca
     6061 cagcctgacg acggcataaa aaggcgccgc cacagcaacc caatatttcc tgattttttt
     6121 gataatcggt taccggttta ttaccgccac gattaaagcc tagaccgttg cgactataat
     6181 ctaagccagc cgaatccatt aactctggtt gaaaataatt aatcattttt ggctggaaaa
     6241 aaccataggc ttgattttcg ggattatcta ccaccactaa taactgatcc agcaaatccg
     6301 gctcaacggt agtgtcatta ttcaagaaaa aaatataacc acaatcagcc tcctgcaaag
     6361 ctctagctac gccgatatta ttaccaccgg caaaaccggt atttttctcg ttcaagatat
     6421 gaatatactc aggaaactca gcggccagtt gtttaccttc atcgttagtt gaagcattgt
     6481 caaccagaat aacctgaaaa tcttgatttt ttaagcgtcg caaagactcc agacattcca
     6541 cggtgtcaga ataaccatta aagttgagaa tgataaaata aattttagat gaaggcatat
     6601 ggatattatt ggttaaaaat ctttttatga ctaagccctg gcaataatag aatcatgggc
     6661 accaacgaat aagaaataac cgttgcccag gcagctccag ataagccgat tattggtatt
     6721 agaataacgt tgagataaat attaagtatc agcgcgctta aataagccat aaaaattatc
     6781 ttattcttgg cctctgaaac caaactaacc caaagaccag tgcccagaaa cattccaatg
     6841 ctagaccagg cataaatttt agcaataggg gtagctggta aaaactgatt accaaaaagc
     6901 agaccaatca gttgtttggc aaaaaacaac ataaaaatag aaaataacag agaaaaagat
     6961 agaagcaaca ttaaaagcca aaattttcga cggcgataaa ccaaccaact atttttctga
     7021 ccattaataa gggctggtaa tacagaattg gaaattaaaa ccggcaaaaa gtaccaaacc
     7081 tctgatagct taacagcaac cgcataatat ccggaagcta atttgccaag catctgaccg
     7141 accatgactt ggtcaatctt aaacaataaa atacaactag cgcttgataa cattaatagc
     7201 caagattctt ttaaaatgtt aaacaacatt tttagagaaa ggcgccaatt taataaaccg
     7261 ccatgaagca catagaaaca atagccaaac atggcccaaa aaacaatatc cagcgaatag
     7321 gccaaaatta aaaatctaac cggagcatca aaaacaacta atgatacttt aattatagcc
     7381 aataaaacaa ttgaaccaat ctggctcaat gatatccact tacttaaaat cctagcttga
     7441 aaccatagat taaatatgtt ggaaaattga aagagaataa ccggaatcga ccagagaaca
     7501 aaataacgat aaccctgttt atccggcaca aggaatagaa tcaaacagca tagtaacgag
     7561 gcgaaaatgc taccagctat ctttaataat aaaccggttc ccaaaacttc tgattcatca
     7621 ccatcattct tagccagctc cctgcttatt atcccatctg cccctaaact agctataaag
     7681 gtgaatatcc cggcgaagct caaggcgtaa cttaaaatac catattcttc tggtcccaag
     7741 tatctagcca cggtcaaacc aacagcaaaa gaaaaaaata aagaaacaaa ttgaccgaca
     7801 aaaagccagc cgatgccttt agaatattta ttggtaatgc cgcgctttaa tagctttgcg
     7861 agattcttaa atcttatcat tttgattgcc aataagtcga ccatagatcg ctaggctgtt
     7921 cttaactatt acacgccagt cataggcgga ttcaactttg gttctagccg ccgaaacaag
     7981 attcttccac aaatcactag atgatagtag cctagatagt tttttaacga attcgtcttg
     8041 acttcgatag attaaaacgt cttgaccgtc aaccaaatca agcccctcag cccctatcg
//
LOCUS       DAOPAI010000023         7856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_8435_length_7856_cov_5.611076, whole genome shotgun sequence.
ACCESSION   DAOPAI010000023 DAOPAI010000000
VERSION     DAOPAI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8435_length_7856_cov_5.611076"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..2586
                     /gene="gyrA"
                     /locus_tag="PKV49_02950"
     CDS             52..2586
                     /gene="gyrA"
                     /locus_tag="PKV49_02950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPL22601.1"
                     /translation="MTKKKSPVSHISPADDHNIEESARPAEITTPHGLILAQPIIEEM
                     RQSYLDYAMSVIVSRALPDVRDGLKPVHRRILYAMWSLGLRSGGKFRKSATVVGEVLG
                     KYHPHGDSAVYDSMARMAQDFAMRYMMVRGQGNFGSVDGDRPAAMRYTEAKLSAIAEE
                     MLFDLDKDTVNMVANFDGSQNEPQVLPAKIPQLLLNGCMGIAVGMATNIPPHNLREVV
                     GAILHLIDNPEATVEELMEFIKGPDFPTGGLIYNREDILRAYTTGKGGIVMRGRAEIV
                     EHGHDNFQIVISEIPYQVNKAILVEKIADLVKDKKIEGIRDLRDESDKDGVRIVIDLK
                     RDSYPKKILNVLYKQTQLQETFHVNMLALVDGLQPRVLNLKTILEEFIKHRQEVVRRR
                     TAYELAKAKDRAHILEGLMIALAHIDEVIATIKASKDKDKAKANLIKQFKLSERQAVA
                     ILEMKLQTLANLETLKIKTELKDKLKTIAELESILSSDSLVLKIIKQECQDIVEKFGD
                     DRRTGVVDQSVKNFKAEDLIPNEEAVVIMTRDGYIKRLPGDTFKVQGRGGKGVIGLTT
                     KEEDSVQFLFTTMTHSNLLFFTSHGRVFELKAYELPLASRTSKGQAIVNFLQLANGEQ
                     VTAILPLDKMANKEYLFFATRRGLVKKVRINAFDNVRRSGLIAIKLKDSDQLIWVKPT
                     TGNDQIQMVTAHGQAIRFHEGDVRDMGRGASGVIGMRLKAKDRIIGMGVIRVDGEKLK
                     DYQVLSVMANGYGKRTSLSQYKVQNRGGSGIKTAKITNKTGELVNAFLVNKKTMEDKD
                     LIIMSKHGQVIRLPFKTVNESGRDTQGVCLMRFKENNDQVVGVTWL"
     gene            2612..3559
                     /locus_tag="PKV49_02955"
     CDS             2612..3559
                     /locus_tag="PKV49_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22602.1"
                     /translation="MKKWLLLSLAAIVMLTGCASRPKNLKPDQAKERAEKFINTALMR
                     PGSQATIKEIELIDGFYKLKVDVGSGDPVDSYLSRDGKLFFPQAMDIDEITQQLSGSS
                     EAESAAATVEKKSDKPVVELFVMSYCPYGLQMERGILPVVEKLGDKIDFKIKFCDYAM
                     HDKPEIDENLAQYCIQKEQNDKFTAYLSCFINSGKASDCYKEAKINETSLQSCISAAD
                     KQFNITASYNNKDAWNGQFPPFDVDKTDNTKYNVQGSPTLIINDQEISVNRDSASLLK
                     TICSAFNNSPEECSAQLSSDSPAAGFTSENNGTAAGSCE"
     gene            3709..5226
                     /locus_tag="PKV49_02960"
     CDS             3709..5226
                     /locus_tag="PKV49_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPL22603.1"
                     /translation="MSAKVLSAGLSGLAAELVEAESDIGSGDIGSFIIVGLPDASISE
                     AKERVRSALRNSGLSLPKLKITINLAPADLRKQGPNYDLPIALSILLASGQLPSGCLR
                     QVMCLGELSLDGQTRPVSGVLPAACLAKELGLRAIIVPAGNAAEAQIVDGLTVWPATD
                     LKQIIGQLRGLSQPLTIKRLDNNSIEPAVVVDFSHVCGQETAKRALEIAAAGNHNLLM
                     IGPPGSGKTMLAKAVPGILTGLAADEAIEVAKIYSIAGYLKGANQPPSIRPFRSPHHS
                     ASAASIIGGGSWPSPGEISLAHRGVLFLDELPEFSRAVLEGLRQPLEDGFATINRTIG
                     QLIFPAQFLLIAAMNPCPCGFFGDNRQVCRCSSRQIANYRRKLSGPLLDRLDLQLTIQ
                     RVRPADFSYGVKLESSAEVRRRVESARQQQKQRLAGTKFLTNGDLTPLAIKQFCLIDR
                     YGQALLRQAVDQLNLSARGYFRVLRLARTIADLANEPTIAKIHLAESLQYRFANY"
     gene            5230..6642
                     /locus_tag="PKV49_02965"
     CDS             5230..6642
                     /locus_tag="PKV49_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPL22604.1"
                     /translation="MWKKISCLSLLAIGLMTSGFGCRLQSSAVKESMQPITLNYWRAW
                     DEPDDFEEILTKYKALHPNITINYRKLRYEEYEKEIINALAEDRGPDIFSIESSWLSR
                     YQAKISPMPDTITMVYPIVKGTIKKETIYELRNSRSLTIKDLRANFVDVVADDVIIDN
                     KIMGLPLSVDTLAMFYRKDLFNNEGIINPPAYWDKNFQDTVKRLTKRDAQGQIIQAGA
                     AIGGGSNVERSADIVAALMLQSRAPVIENGRVVFNRQSSDGSNYYPGIDAVRFYSDFA
                     NINKEVYCWDKSLGNSFDLFVRGQAAIMFGYSYHIPLIIAARRTGFTGGDAGQYLKDL
                     NIGISKLPQIASTSQPVNIANYWVETVSKKSQHSNEAWDFIQFMTKAENVGSYLDKTQ
                     RPTALRSLIEEQLSKEYLDVFASQLLTARSWYRGYDSAMAHKVIGEMIDGVLNAPDQL
                     EAIINLAIGKLQQTIAAPSQ"
     gene            6652..7056
                     /locus_tag="PKV49_02970"
     CDS             6652..7056
                     /locus_tag="PKV49_02970"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22605.1"
                     /translation="MKIYKLFCLLAIITFCFLPVTVLAQERQIVPTEGYEDGNYTIAS
                     FETMIIFVGKAILGLSGTFALVMFVWGGIQMITAAGRSKAFESGRDTVVNALIGLAII
                     LCSYLIINFVLNIVGVKQSWPRDKIDQVMGNK"
     gene            7079..7732
                     /locus_tag="PKV49_02975"
     CDS             7079..7732
                     /locus_tag="PKV49_02975"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPL22606.1"
                     /translation="MSSRTTSNFGSVIKDRRLAKNWSVLFCARRLKIKPEHIRAIEDN
                     NFDHLPVRVNLVKVVVDYLQMLGFSAEEAAPIIDCWRSSDNSQRDNFFGRRLVRKRDL
                     WSYPQIVRNSLVALAILVAAIYIVLSLKNIVAPPKLLVTSPAGDLATNQKQLWIVGQT
                     EPEVQLEINGETLLSDRVGNFSQLVNLRSGINNLSITAVKKYGGRTTIVRQVMVTDN"
     gene            7784..7855
                     /locus_tag="PKV49_02980"
     tRNA            7784..7855
                     /locus_tag="PKV49_02980"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7817..7819,aa:Arg,seq:tct)
BASE COUNT         2162 a         1421 c         1881 g         2392 t
ORIGIN      
        1 acgccaaaaa ttagagaaat tacctaatca gtcaaccgat taaccaagtt tatgaccaaa
       61 aaaaaatcac cagtttccca tatcagccca gctgatgatc acaacattga ggaatcagcc
      121 cgaccagctg aaataaccac tccacatggt ttgattttag ctcaaccgat tattgaggaa
      181 atgcggcagt cctatttgga ctatgccatg agtgttattg tttctcgggc actgcctgat
      241 gttcgtgatg gtctcaagcc agtgcatcgg cggattcttt atgctatgtg gtcactgggt
      301 ttgcgttctg gcggcaaatt tcgtaaatca gccactgtag tcggtgaagt gcttggtaaa
      361 tatcatccgc atggcgatag cgccgtttac gactcaatgg cgcgaatggc tcaggatttt
      421 gctatgcgtt atatgatggt ccgcggtcaa ggcaacttcg gctcagttga cggtgatcgg
      481 ccggcggcca tgcgttatac cgaggccaaa ttatcggcca ttgctgaaga gatgttgttt
      541 gatttagaca aagacacggt caatatggtt gctaattttg acggatcgca aaatgaaccg
      601 caagttttac cagccaagat tccgcagttg ttgctcaatg gatgcatggg tattgctgtt
      661 ggtatggcta ccaatattcc gccccacaat ttgcgtgagg tggtcggcgc tattttgcat
      721 ttaattgaca accctgaagc taccgttgaa gaattaatgg agtttattaa gggccctgat
      781 ttcccgactg gcggcttaat ttataaccga gaagatattt tacgagccta taccactggc
      841 aaaggaggga ttgtgatgcg tggtcgggct gaaattgttg aacatggtca cgataatttt
      901 cagattgtta tcagcgagat tccttatcag gttaataaag caattttggt ggaaaagatt
      961 gctgatttag tcaaagacaa aaaaattgaa ggcatccgtg atttgcgtga tgaatcggac
     1021 aaagacggtg ttagaatcgt tattgatctt aagagagata gttacccgaa gaagatttta
     1081 aatgttttat ataagcaaac ccagctgcaa gaaacttttc atgttaacat gctggccttg
     1141 gtggatggac tacagcctcg agttttaaat cttaaaacca ttttagaaga atttattaaa
     1201 catcgccaag aggtggtgcg ccgtcggact gcttatgagt tggccaaagc taaggatcgg
     1261 gcccatattt tggaaggctt aatgattgct ttagcccata ttgatgaagt tattgccact
     1321 attaaggcct ctaaagacaa agataaggcc aaggctaatt taattaagca gtttaagcta
     1381 tctgagcggc aagctgtggc cattctggaa atgaagctac agacattagc taatctcgaa
     1441 acattaaaga ttaaaactga actcaaggat aaattaaaaa ctattgccga gttagagagt
     1501 attttatcgt ccgatagttt ggtgttaaag attattaagc aggaatgcca ggatattgtt
     1561 gaaaagtttg gcgatgatcg tcgtaccggc gttgtagacc aaagtgttaa gaattttaag
     1621 gctgaagatt tgattcccaa tgaagaagcg gtagtgatta tgactaggga tggctatatc
     1681 aagcgtttgc ctggcgatac ctttaaggtt cagggccgtg gcggtaaagg cgtaattggt
     1741 ttaaccacta aagaagaaga ttcagtgcaa tttttgttta ctaccatgac tcatagtaat
     1801 ttgttattct ttaccagtca tggtcgagtt tttgaattaa aggcttatga attgccttta
     1861 gcctccagaa catccaaagg gcaggcgatc gttaattttt tgcaattagc taatggcgag
     1921 caagttaccg ctattttacc actagataag atggctaata aggagtatct gttttttgct
     1981 acgcggcgcg gcttagttaa gaaagtccgc atcaatgctt ttgataatgt ccggcgcagt
     2041 gggttaattg ctatcaaact taaggacagc gatcaattga tttgggttaa gccgaccacc
     2101 ggtaatgatc agattcaaat ggtaaccgct catggtcaag ccattcgttt ccacgaaggg
     2161 gatgttcgcg atatggggcg tggcgccagt ggtgtaattg gcatgcggct caaggctaaa
     2221 gatcggatta tcggtatggg agttattagg gttgatggcg aaaaattgaa agattatcaa
     2281 gttttgtcag tcatggctaa tggttatggc aagcggacca gtctgtctca gtataaggtt
     2341 caaaatcgtg gcggatcggg tattaagaca gccaaaatta ccaataaaac cggtgaatta
     2401 gtcaatgctt tcttggttaa taaaaaaacc atggaagaca aagacttgat tattatgtct
     2461 aagcatggtc aagtcatccg tctgcctttt aagacagtta atgaatccgg tcgcgacact
     2521 caaggtgttt gcttgatgcg ttttaaagaa aataatgacc aggtagttgg ggtaacctgg
     2581 ctttagatat taattattta agtttttatt tatgaaaaaa tggttattgc tgagtttggc
     2641 cgccattgtc atgctgactg gttgcgctag tcggcccaag aatttaaagc ccgatcaagc
     2701 caaagaacga gcggaaaaat ttattaatac tgctctgatg cggccgggta gccaggcgac
     2761 tatcaaagag atagagttga ttgatggttt ttataaatta aaagtggatg ttggtagcgg
     2821 cgacccagtt gattcttatt tgagtcgtga tggtaaatta tttttcccgc aagctatgga
     2881 tattgacgaa ataacacaac aattatccgg cagttccgaa gctgaatcag cggccgctac
     2941 tgttgagaaa aaaagcgaca aaccggttgt ggaactattt gttatgagct attgtcctta
     3001 tggtttacag atggaacgag gcatattgcc ggttgttgag aaattgggcg acaaaattga
     3061 ttttaaaatt aagttctgcg actatgctat gcatgataaa ccggaaattg acgagaattt
     3121 ggcccaatat tgtattcaaa aagaacaaaa tgataagttt accgcttatt tgagttgttt
     3181 tattaactct gggaaagcca gtgattgcta taaggaggct aagattaatg aaacttcttt
     3241 gcaatcttgt attagcgctg ctgataaaca atttaatatt acggctagtt ataacaataa
     3301 agatgcttgg aatgggcaat tcccaccatt tgatgtcgat aaaacagaca ataccaaata
     3361 taatgtccaa gggtcgccga ctttgattat taatgatcaa gaaattagcg ttaatcgtga
     3421 tagcgccagc ttgttaaaga ctatttgctc agcttttaat aattcgcccg aagaatgttc
     3481 ggctcagtta tctagtgata gtccggctgc cgggtttact tcagagaata atggaacagc
     3541 ggctggtagt tgcgaatagc gataataata tatagttgtc gttttagaaa aaatcggtca
     3601 gattggccga ttttttcttt ttaggctata atgcagtagc taattcttaa tctcaggagg
     3661 ggatgaagcg gcggcttgcc ggttcatatt cacctgttct ttttaattat gtcagccaaa
     3721 gttttatcag ctggccttag tggcttagcg gctgagctag tggaggccga atccgatatt
     3781 gggagcggcg atatcggctc ttttattatt gttggattgc ctgatgcttc aatttcggag
     3841 gcgaaagagc gggtccgtag tgcgttgcgt aattcaggtt tatcgttgcc gaagcttaaa
     3901 attacgatta atttagctcc ggctgattta cgcaaacagg gtccgaatta tgatttgcca
     3961 attgcgctta gtattttatt ggctagcggt cagttgccca gcggttgttt gcgacaggtt
     4021 atgtgtttgg gcgaattgtc gcttgacgga caaactcgtc cagtgagcgg tgttttgccg
     4081 gccgcttgtt tagccaaaga gcttggactg cgagctatta ttgtgccggc gggcaacgct
     4141 gcggaagcgc aaatcgtcga tggattaacc gtttggccag ctactgattt aaaacagatt
     4201 attgggcagt tacgaggttt aagccagcct ctaactatta agagattgga taataattca
     4261 attgaaccag ctgttgttgt tgattttagc catgtatgtg gtcaagagac cgccaagcgg
     4321 gcgctagaga ttgccgctgc cggtaaccat aatttgttaa tgatcggtcc gccgggatca
     4381 ggtaagacta tgctggctaa agctgttcca ggtatcttga ctggtctggc cgctgatgag
     4441 gcgattgagg tggctaagat ttatagtatt gccggctatc tcaagggagc taatcagccg
     4501 cccagcattc ggccttttcg tagtccgcat catagcgctt cagcagcttc aattattggc
     4561 ggcggttcat ggcctagccc gggagagatt tctttggctc atcgcggcgt gcttttttta
     4621 gatgaactgc cagaattttc tcgcgccgtc ttagagggct taaggcagcc gctcgaagat
     4681 ggttttgcca ccattaatcg gacgattggc cagttgattt ttccagcaca gtttttattg
     4741 atagctgcca tgaatccttg cccttgcggg ttttttggtg ataatcggca agtttgccgc
     4801 tgtagctcgc ggcagattgc taattatcgc cgtaaattat ctggaccatt gcttgatcgt
     4861 ctagatttac agttgacaat ccaacgagtt cgtccggctg atttttctta tggcgttaaa
     4921 ctcgaaagct cggccgaagt tagacgaagg gttgagtcgg ctcgtcagca acaaaaacag
     4981 cgtttggccg gcaccaaatt tttgactaat ggcgatttaa cgccactagc gataaaacaa
     5041 ttttgtttga ttgatcgtta tggccaagcc ttattacgcc aggcagtcga tcagttaaac
     5101 ctatcggcgc gcggctattt tcgagtttta cgtttagcca gaaccattgc cgatttggct
     5161 aatgagccaa caattgctaa aatacattta gcagagagtc tacaatatcg ttttgctaat
     5221 tattaattta tgtggaaaaa aatcagctgc ttatctttat tggccatcgg tttgatgact
     5281 agcggttttg gctgtcgtct tcaatctagt gctgtcaaag aaagtatgca gccaattact
     5341 ctcaactatt ggcgagcctg ggatgagccg gatgattttg aagaaatttt aactaagtat
     5401 aaggcgttgc atccaaatat caccattaat tatcgcaagc tgcgttatga ggaatatgaa
     5461 aaggagatca ttaatgctct agctgaagat cgcggtccag atattttttc tattgaaagt
     5521 agttggctat ctcgttacca agccaaaatt tcaccgatgc ccgatactat tactatggtt
     5581 tatccaatag ttaaggggac gattaaaaag gaaacaatct acgaattaag aaatagtcgc
     5641 agcctgacga ttaaggatct gcgagccaat tttgtggatg tagtcgctga tgatgttatt
     5701 attgataata agattatggg tttgccgtta tcggttgata cgctagctat gttttatcgt
     5761 aaagatcttt ttaataatga gggtattatt aatccacccg cctattggga taagaatttt
     5821 caggatacag tcaagcgttt aaccaagcgc gatgctcagg gtcaaattat tcaagctggc
     5881 gcggctatcg gcggcggcag taatgtggaa agatcggctg atatcgtggc cgctttaatg
     5941 ctgcaaagtc gcgccccagt tattgagaat ggccgcgtag tttttaatcg tcaatcgtcg
     6001 gatggttcca actactatcc tggtattgat gctgttcgtt tttatagcga ttttgctaat
     6061 attaacaaag aagtttattg ttgggacaag agcctaggta actcttttga tttatttgtg
     6121 cgtggccaag ccgcgattat gtttggctat agttatcaca taccattgat tatcgctgcc
     6181 cgtcgcaccg gttttactgg cggtgatgcc ggtcaatatc ttaaagattt aaatattggt
     6241 attagtaaat taccacaaat cgccagcact agtcagccag ttaatatcgc taactattgg
     6301 gtggaaactg tttccaaaaa gagccagcat agcaatgagg cttgggattt tattcaattt
     6361 atgaccaagg ctgaaaatgt cggcagttat ttggataaaa ctcagcgacc gacagcttta
     6421 cgcagcttaa ttgaagaaca attgtctaag gagtatttgg atgtttttgc tagtcagcta
     6481 ttaacagcgc gtagttggta tcgtggttat gattctgcca tggcacataa ggttatcggc
     6541 gaaatgattg atggcgtttt aaacgcgccc gatcagcttg aagcgattat taatttagcc
     6601 attggcaaat tacagcaaac catagcggcg cccagccaat aaaaaaaata tatgaaaatt
     6661 tataaattgt tctgtttgtt agctattatc acgttttgtt ttttacctgt tacggttcta
     6721 gctcaagagc ggcaaatagt gccgactgaa ggttatgaag acggcaacta tactatagct
     6781 tcttttgaga ctatgattat ttttgttggt aaagctatat tagggctttc tggcaccttt
     6841 gctttggtta tgtttgtttg gggcggaatc cagatgatta ctgctgcggg tcgatccaag
     6901 gcttttgaaa gtggtcgtga tacggttgtt aatgcattaa tcggtttagc gattattttg
     6961 tgcagttatc tgattatcaa ttttgtttta aatattgttg gggttaaaca atcttggccg
     7021 agagacaaaa ttgaccaggt aatggggaat aaatagctga cggcgattat taatatttat
     7081 gtctagtagg acgaccagta attttggttc ggttatcaaa gatcggcgtc tcgccaagaa
     7141 ttggtcagtc ctattttgcg ctcgacggct taagattaag cctgaacata ttcgagctat
     7201 tgaagataat aattttgatc atttgccggt cagggttaat ttggttaagg ttgttgttga
     7261 ttatttacaa atgcttgggt tctcagccga agaagcagcc ccaatcattg attgttggcg
     7321 gagtagtgat aacagccaac gggataattt ttttggccgc cgtttagtaa gaaaaagaga
     7381 tttatggtca tatccgcaga ttgttcgtaa tagtctagtc gcattagcga tcttggtggc
     7441 ggctatttat attgttttat ctttaaaaaa tattgttgcc ccgcctaaat tattggttac
     7501 tagtccggct ggcgatctgg ccaccaatca aaaacaatta tggattgttg gtcaaaccga
     7561 gccagaagtt caattggaga tcaatggtga gacattatta agcgatcggg tcggcaattt
     7621 tagccaactg gttaatctgc gttccggtat taataatttg agcataacag ccgtcaaaaa
     7681 atatggtggc cgaaccacca ttgtccgtca agttatggtg acggataatt gattaattta
     7741 cattgacaaa agggggtgcc cctgttaaat tggacatagt ttggcgccca tagttcaatg
     7801 gatagaacac cagccttcta agctggttat gagggttcga ttccccctgg gcgtac
//
LOCUS       DAOPAI010000024         7754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_8546_length_7754_cov_5.797636, whole genome shotgun sequence.
ACCESSION   DAOPAI010000024 DAOPAI010000000
VERSION     DAOPAI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8546_length_7754_cov_5.797636"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1221)
                     /locus_tag="PKV49_02985"
     CDS             complement(268..1221)
                     /locus_tag="PKV49_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22607.1"
                     /translation="MSKRSAIRRDYLLAQEVIISPGRAARPKNHKATKPKIDQRVATS
                     CFFCPEKLDYNNVIELLPPNSAKKWQVAAINNIYPAVEMDNQQALGKQEVIIDTPRHE
                     QSLADVTIDELAMVLKMYAQRTIALSKQPPLDYILCFKNYGHDAGASLEHSHSQVFAT
                     KILPLAIRAELSAARRYRRRTGHCPYCDMIAKEVMAAERLIWQDHNVAVIAPYASRFP
                     YEAWIFPKRHQDNIGQASQVELRSLASGLSLILKQLQTLGMAYNICLHQVISATDQHF
                     VIKVQPRGNIWAGVEIGSGLIVNSMPPEEATVFYRSNLSNI"
     gene            complement(1348..3246)
                     /locus_tag="PKV49_02990"
     CDS             complement(1348..3246)
                     /locus_tag="PKV49_02990"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPL22608.1"
                     /translation="MYKSTPLPLLHDYFLNYQLTHKKAMARKKTSLEAVLNLAIVFLV
                     VIFCAQLLSAWRFEADAHDLVGRAQQLLSVPAALAAEPVNIAEVTPYTTNLILKSGQE
                     ATINLSFVNRGQTVWPAGQTTLETGPFLKTASKVQHASWVSFFKVLSLSKNVKPGEKI
                     VISFKIAGPEAKYVGVVQENFQLVYNQQPIKNSLARVFIDANQVKPTFAVNSSNSSLT
                     TPAVKAAVTSPVVKVQYITGYDKLGQVVKVAKGQYVPGISLTKPSAAAIAAEKARLAA
                     VASQPKTAPVVPATPVADDVFCIALSAAERAVSPRCQTSNNESSAVNGLVNYARKLSQ
                     QPLIRVGLFNSNQSQRITSEQSFDIWAADNLLVSAVPANTIVSVNYSRDNRQYNISVG
                     NNNYQTANYLRFVSRSDNDGFFQLMDYRNNPNWSASFNDNKFRRVLEYRYTPKTDKIW
                     LINELPIDSYLKGLAETSNASPVEFQKVLATAARTYVLYHYYRGIDFNLPGASTKHAD
                     EYFHVDATYDQVYRGYNSEIRMPRLAQAVDETAGAIVTYNNELAITPYFSRSDGRTRS
                     WNEVWGGGEKAWLQSVVVPEDSGKTLFGHGVGMSAQGALLMIVDSHQTWQEVLAYFYQ
                     GTNLQQAY"
     gene            3468..4856
                     /gene="tig"
                     /locus_tag="PKV49_02995"
     CDS             3468..4856
                     /gene="tig"
                     /locus_tag="PKV49_02995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPL22609.1"
                     /translation="MQVNVQEKKSQLKLTVELSTEEFQPYILEGARRIASEIKIPGFR
                     NGKAPCNLIKAKVGEMTILEEAARVAINRTIDEAIKKGADEKMVVGQPQVDITKLAPG
                     NPLEYKIKLTLLPKTVINQYKELNIKQKAPKIPETQINRTLDQLREANVQKTPSLEPV
                     KMGDLVIADIHISVDKVPVEGGQGHGVNIILGKDYLVPGFDRQLLGANIGQELKFQIN
                     YPANHYQKTLAGKLVDFKVVITEVMHRRLPELDDQLAKSFGFEKLSDLKKNIAESLAA
                     EQKKQLDHQAEAEMFEKLIKLATIEELPETLINRETEVMLDELQHNLNQNGGNLKDYL
                     NNLKKTKEQFVLEILPQAIKRVKTALIVNEVARLEKITITAEEVDQELEQLKKHYQDN
                     QEILKRLSTPGYKEYLANALVSNKTVRALRGWNIINEAPVEEKKPVTATKNTAKKSVA
                     KRRTTTAKKPTK"
     gene            4857..5324
                     /gene="smpB"
                     /locus_tag="PKV49_03000"
     CDS             4857..5324
                     /gene="smpB"
                     /locus_tag="PKV49_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPL22610.1"
                     /translation="MMPVVAVNKRASYDYEILENYEAGLVLTGYEVKAAKAGQVSLKG
                     SHILFRSGQHGQPQAYLINAHISLYRLAGAIGDYNPTRNRRLLLNKKQLNHLYGRAST
                     EGLTVVPLKLYTKDSLLKLAIALVRGKKKFDKRQAIKERDWERKRRTLTKQAI"
     gene            5327..5709
                     /gene="ssrA"
                     /locus_tag="PKV49_03005"
     tmRNA           5327..5709
                     /gene="ssrA"
                     /locus_tag="PKV49_03005"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5730..5815
                     /locus_tag="PKV49_03010"
     tRNA            5730..5815
                     /locus_tag="PKV49_03010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5764..5766,aa:Leu,seq:taa)
     gene            5836..5907
                     /locus_tag="PKV49_03015"
     tRNA            5836..5907
                     /locus_tag="PKV49_03015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5869..5871,aa:Gly,seq:gcc)
     gene            5944..6498
                     /gene="infC"
                     /locus_tag="PKV49_03020"
     CDS             5944..6498
                     /gene="infC"
                     /locus_tag="PKV49_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPL22611.1"
                     /translation="MRRTFRKPKPKEEKKFRTNYQITLPEVFVIDENGQSLGVIPTSE
                     AIRLAEAAELDLVEVNPNNNPPVTKILDYGQFRYHVEKKLQHQKAKQKKIDMKEVRLS
                     IRIGDHDFNTRVDQGQKFLNKGNKLKITVTIRGRERQHPEKAEETLDRYFKQLEGLME
                     NPLSIEEPLTKLNGRFSMVVVNKK"
     gene            6560..6757
                     /gene="rpmI"
                     /locus_tag="PKV49_03025"
     CDS             6560..6757
                     /gene="rpmI"
                     /locus_tag="PKV49_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPL22612.1"
                     /translation="MPKLKTYQAAAKRVSLTKTGKLMRRKAGQDHFNAREDGKTGRIK
                     RRDLTIASADKKNIQNLLPYR"
     gene            6781..7128
                     /gene="rplT"
                     /locus_tag="PKV49_03030"
     CDS             6781..7128
                     /gene="rplT"
                     /locus_tag="PKV49_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPL22613.1"
                     /translation="MTRVKRGTSHTKRRASLLSKVKGFRWGRKNLINLASAAATKAGV
                     NAYCDRRKKKADFRALWQIKIGAFAKQHNLSYSRLMALLKKANIALNRKSLADLAANH
                     PEILAEIIEQARS"
     gene            7138..7356
                     /gene="secG"
                     /locus_tag="PKV49_03035"
     CDS             7138..7356
                     /gene="secG"
                     /locus_tag="PKV49_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPL22614.1"
                     /translation="MRWLNIGQIIVSILLILVILMQNRGSSMSSLFGGSGDIYRTKRG
                     IEKTLFIGTIVLLVIFFGLSIANLMINQ"
     gene            7360..7659
                     /locus_tag="PKV49_03040"
     CDS             7360..7659
                     /locus_tag="PKV49_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPL22615.1"
                     /translation="MNQDKINKVRLIEKIAEKINVNKKQIEQTIDSLLEITINELQSG
                     NTVTLVGFGSFIPKKRHARGGVNPQKPQERINIPEVTVAKFKTGKALKDALKNKR"
BASE COUNT         2526 a         1660 c         1525 g         2043 t
ORIGIN      
        1 gcatgcctta gacacgccgc cagcgttcat cctgagccag gatcaaactc tcaattaata
       61 ttgaagagaa tgtttagctc tcttataaag aatagttttt gaattagcgc ctgttagcgc
      121 taaaatcgtc tgctattgtt aatgttcaac caaaaaatgg attattaaaa atacttatct
      181 aacaatagtt aaacaagtat ctgtaatgat aaatgatttt accatgactg tcaagagtcc
      241 taaaatactg gttattcgct gctctgccta aatattactc aaatttgacc gataaaaaac
      301 agtagcctct tctggcggca ttgaattaac tattaatccc gaaccaatct caactccagc
      361 ccaaatatta ccgcgcggct gaaccttaat aacaaaatgt tgatcagtgg ccgagattac
      421 ttggtgcaaa caaatattat aagccattcc taaagtttgt agctgcttta gaatcaaact
      481 aagaccactg gccaaagacc gaagctccac ctggctagct tggccgatat tgtcttgatg
      541 ccgcttaggg aatatccagg cctcatacgg aaagcgactg gcatacggag caatgactgc
      601 cacattatga tcttgccaaa ttaagcgttc agctgccata acttctttgg caatcatatc
      661 gcaatacggg caatgaccgg tccgccgacg atagcgccgg gcggctgata attcggcgcg
      721 aatagccagc ggcaatatct tggtagcaaa aacttgtgag tgagagtgtt ccagcgaagc
      781 cccagcgtca tggccataat tcttaaaaca aagaatataa tctaatggcg gctgtttact
      841 cagcgcaatg gtccgctgag catacatttt taacaccata gctaattcgt caatagtcac
      901 atctgccaat gattgttcat ggcgtggcgt atcaattata acttcttgct tacctagagc
      961 ctgctgatta tccatctcca ctgccggata aatattatta atagccgcta cctgccattt
     1021 cttagctgaa ttaggcggca ataattcaat aacattatta taatccaact tttccggaca
     1081 aaaaaaacaa ctagtggcca cgcgctgatc aattttaggt ttggtggctt tatgattttt
     1141 tggtcgcgcc gcccgacctg gactaataat tacttcttgc gctaaaagat aatcgcgacg
     1201 aattgctgat cgcttactca tatattaaaa ataaaaatta ttattactat aataacacaa
     1261 ataagcacca aaacaaaaac cgacccccgg gaatcaatgt ctttttgcct gatacctggg
     1321 agccggttag ttgtttatga ttaattatta ataagcttgt tgtaggtttg ttccttgata
     1381 aaaataagcc aatacctctt gccaagtctg gtggctatca acaatcatta ataaggcgcc
     1441 ttgagcgctc atgccaacac catggccaaa cagcgtttta ccactatctt ctggcacaac
     1501 cactgattgc aaccaagctt tttcgccgcc gccccaaacc tcattccagc tgcgggttcg
     1561 accatcactg cgcgaaaaat atggagtaat agccaactca ttattataag tcacgatagc
     1621 gccagctgtt tcatcaaccg cttgagccaa gcgcggcata cggatttctg aattataacc
     1681 gcgataaacc tgatcataag tagcatcaac atgaaaatat tcatcagcat gcttggtgga
     1741 agcgccaggg aggttaaaat caatgccgcg ataatagtga tataagacat aagtgcgagc
     1801 agcagtagct aaaactttct gaaattccac tggtgaagca ttactagttt ccgccaaacc
     1861 tttcaaatag ctatcaatcg gtaattcatt aatcagccaa atcttatcgg ttttgggcgt
     1921 ataacgatat tctaagacgc gacgaaactt attgtcatta aaactagcgc tccagttagg
     1981 attattacga taatccatca gttgaaaaaa gccatcatta tcagaccgac taacaaacct
     2041 taaataatta gcagtttgat aattattgtt gccgacagaa atattatatt gacgattatc
     2101 tcgagaataa ttaaccgaga caatagtgtt agccggcaca gcactgacta ataaattgtc
     2161 agctgcccaa atatcaaaag actgttcact ggtaattctc tgcgattgat tactattaaa
     2221 taaaccaact ctaattaatg gttgttgact taattttctg gcataattaa ccagaccatt
     2281 aaccgctgag ctctcgttgt tactggtttg acagcgtggc gaaactgccc gctcagccgc
     2341 ggataaagca atacaaaaga catcgtcagc gaccggagtg gctggcacaa ctggcgcagt
     2401 tttgggctgg ctagcaacag cggctaaacg agccttctca gcagcaatcg ccgccgccga
     2461 tggtttagtt aaagaaatac caggcacata ctgtccttta gccactttaa ccacttgtcc
     2521 taacttatca taaccggtaa tatactgaac cttaacaact gggctggtta cggcagcctt
     2581 gactgccgga gtggtcaaac tgctattact agaattaacg gcaaaagttg gtttaacctg
     2641 attagcatca ataaaaacac gagccaaact attcttaatc ggttgctgat tataaactaa
     2701 ttgaaaattc tcctgcacca caccgacata tttagcctct ggcccggcaa tcttaaaact
     2761 aattacaatt ttttcgcccg gcttaacatt cttagacaag cttaaaacct taaaaaaaga
     2821 cacccagcta gcatgctgga ctttggaagc agtttttaaa aatggtccag tctctaaagt
     2881 tgtttgtccg gctggccaaa cagtctgccc gcgattaaca aaacttaaat taatagtggc
     2941 ctcttgcccg gattttagaa ttaggttagt tgtataagga gtcacttcag caatattaac
     3001 cggttcggcg gctaaggcgg ctggcactga caataactgc tgcgcccgac cgactaaatc
     3061 atgggcatca gcctcaaaac gccaagcgct taacagctga gcgcaaaaaa taaccactaa
     3121 aaaaacgatg gccaaattaa gaaccgcctc aaggctggtt ttctttctgg ccatcgcttt
     3181 tttgtgagtt aattgataat ttaaaaaata atcatgaagg agtggaagtg gagttgattt
     3241 gtacatattt ttttgttttt tgttggcttg gtttattttt tttcaccagc aactgccagc
     3301 agtatagcaa aaaaaacgag aaaagtcaag attaatgtag acttatcttt ataattaagt
     3361 tgccaactta gcaaaagctt gttaaactaa cttcactacc ttgaccacca agacttaggt
     3421 tggtttttag ctataaaaaa aaataaatat ataaataatt tatcaatatg caagttaatg
     3481 tccaagaaaa aaaatcccaa ctaaaattaa cagttgagtt gtcgaccgaa gaattccagc
     3541 cctatatttt agagggcgct cgccgcattg ccagcgaaat taaaatcccc ggcttccgca
     3601 acggcaaagc gccatgtaat ttaattaaag ctaaggttgg cgaaatgacc attttagaag
     3661 aagcggcccg agtcgccatt aatcgaacaa ttgatgaggc gatcaaaaaa ggcgctgacg
     3721 aaaaaatggt cgtcggtcag ccgcaagtag atatcactaa actagccccg ggtaatccgc
     3781 tagaatataa aattaagcta accttgctgc ccaaaaccgt cattaaccaa tataaggagc
     3841 ttaatattaa acaaaaggca ccaaaaattc ccgaaacgca aattaaccgg acactagacc
     3901 aattgcggga agctaacgtc caaaaaaccc ccagtttgga gccggtcaaa atgggtgatt
     3961 tggtaattgc tgatatccat atttcagtag ataaagttcc ggttgaaggc ggtcaaggtc
     4021 atggcgttaa tattatttta ggtaaagact atctagtgcc tggttttgat cggcagctgc
     4081 tcggagccaa tatcggccaa gaattaaaat ttcaaatcaa ttatccagct aatcattacc
     4141 aaaaaacact ggctggcaag ctagttgact tcaaggtggt tattactgaa gtaatgcatc
     4201 gccgcttgcc cgagctagac gaccagttgg ccaagagttt tggttttgaa aaactaagcg
     4261 atctcaaaaa aaatatcgct gaaagcttgg cggctgaaca aaaaaagcag cttgaccacc
     4321 aggcagaagc agaaatgttt gaaaaattga ttaaactagc cactattgag gaattaccgg
     4381 aaactttaat caatcgtgaa accgaagtta tgctggatga attacagcac aacctaaacc
     4441 aaaacggcgg caatcttaaa gattatctca ataatcttaa aaaaaccaag gaacaatttg
     4501 ttctggaaat cttacctcag gcaatcaaac gtgttaaaac tgctctaatt gtcaacgaag
     4561 tagctagatt agaaaaaatt accataacgg ctgaagaagt tgatcaagaa ttggagcaac
     4621 tcaaaaaaca ttaccaagac aaccaagaga ttcttaaacg gctgtcgact ccgggttaca
     4681 aggaatattt agctaacgcc ctagtcagca acaaaacggt tcgagcgctg cgtggctgga
     4741 atattatcaa tgaagcacca gttgaagaaa aaaaaccagt aaccgccacc aagaatactg
     4801 ccaaaaaatc agtggccaaa cgccgaacta caaccgccaa aaagccgacc aaataaatta
     4861 tgcctgttgt cgccgttaat aaacgagcca gctatgatta tgaaatcctg gaaaattacg
     4921 aggcaggatt agtgttaacc ggctacgaag ttaaggcagc caaagctgga caggttagtt
     4981 taaagggcag ccatatcctt ttccgcagcg gccaacatgg ccaaccgcaa gcctatttaa
     5041 taaatgccca tatcagtctt tatcgtctag ctggcgctat tggcgactat aatccgaccc
     5101 gtaatcgtcg gctgctatta aataaaaaac aactcaatca tttatacggc cgagcctcaa
     5161 ccgagggctt gacagtcgta ccgctcaaac tatatactaa ggacagtttg ctcaaactgg
     5221 ctattgcttt agtccgcgga aagaaaaaat ttgacaagcg ccaagccatt aaagagcgcg
     5281 attgggaaag aaagcgccga actttgacaa agcaagcaat atagttgggg atgctgggtt
     5341 tcgctcatga gatcgcggtg tccgattggg cgaataggtg ctgatttcac ctaaaacaat
     5401 caacaatata gatgccaaaa acatcatggc gaaagcagcc ttccggttgt cttttgcacc
     5461 agttgccgct taaatagccg caaccatcga cgctaatgat gcctaataat tagttgtcgg
     5521 tgttatttta ttaggctgtg gcttggaatc aatccgcctg gttgaaacca agacaagaat
     5581 ctaatcgggt cgctctggaa atttttgtct aatttctcat ttccaaagtg tacaaaaatt
     5641 agactattat tcgtagaaca gtcggttacc tcttgtgata caggggttca actcccctca
     5701 tctccacatt atcactgatt aacagattgg ccgcgatggc ggaactggta gacgcatcgg
     5761 acttaaaatc cgacgatctt aaaacatcgt gagggttcga gtccctctcg cggcacgctt
     5821 ttcaacaata atattgcggg tatcgtataa tggctattac attaccttgc catggtagag
     5881 atacgggttc gattcccgtt acccgctcac actatcaact ctaatcaatt ataattacca
     5941 gccatgcggc gaacttttcg caaaccaaaa ccaaaagaag aaaaaaaatt ccgcactaat
     6001 taccagatta ctttgccgga agtttttgtt attgacgaaa acggccaatc gttaggagtt
     6061 atcccgacta gcgaagctat ccgtctagca gaggcggcgg aattagattt agttgaggtt
     6121 aatcctaata acaacccacc ggttaccaaa attctagatt atggccaatt ccgctatcat
     6181 gtggaaaaaa agctgcagca tcagaaggcc aaacagaaaa aaattgacat gaaagaggtt
     6241 cgtttatcaa tccgcattgg tgaccatgat tttaatactc gcgtcgacca gggccaaaaa
     6301 tttctaaata aaggcaataa attaaaaata accgtcacca ttagaggacg agaaagacaa
     6361 catccggaaa aggccgaaga aacattagac cgctatttta aacagcttga aggattgatg
     6421 gaaaacccat tatctattga ggaacccttg acaaagctga atggaagatt tagtatggta
     6481 gtggtaaata aaaaatagcc gattatttca attattgact gaaattaggt gtaaaaacct
     6541 atttttatta ttctaaaata tgccgaaact caagacctac caggccgctg ctaaacgggt
     6601 atccctaacc aagaccggca aacttatgcg ccgcaaagct gggcaggatc attttaatgc
     6661 ccgcgaagac ggcaagactg gccgtatcaa acggcgcgat ctgacgattg cctcagctga
     6721 taaaaaaaat attcagaatc ttttgcccta tcgctaggca ggttctctta aactaccaat
     6781 atgactagag ttaaacgagg aacttctcat accaaacgtc gcgctagcct actatccaaa
     6841 gtgaaaggtt ttcgctgggg ccgtaagaat ttaatcaact tagccagcgc ggcggcgact
     6901 aaggctggcg tcaatgctta ttgtgatcgc cgcaaaaaga aagctgattt tcgagctttg
     6961 tggcaaatta aaattggcgc ttttgctaag cagcataatt tatcttattc tcgtctgatg
     7021 gctttattga aaaaagccaa tattgcgtta aatcgcaaga gtttagctga tttagcggct
     7081 aatcacccag aaattttagc ggaaattatt gaacaagcgc gttcataaac ccaaattatg
     7141 agatggctta atattggaca aattatcgtt tctatcttgc ttattctggt tatcttaatg
     7201 cagaatcgcg gatctagcat gtccagctta tttggcggca gcggtgatat ttatcgcacc
     7261 aaacgaggta ttgaaaaaac tttatttatt gggaccatcg ttctgttagt aattttcttc
     7321 ggcctgtcaa tagctaattt gatgattaat caatagagta tgaaccaaga caaaataaac
     7381 aaagtccgtt tgattgaaaa aattgctgaa aagattaatg ttaacaaaaa acagattgag
     7441 caaaccattg actcattact ggagataaca attaatgaac tgcaatccgg caacacggta
     7501 actttggtcg gtttcggtag ttttatcccc aaaaaacgcc acgctcgcgg cggcgtcaat
     7561 ccgcaaaaac cacaagaaag aattaatatt ccagaagtaa cggtggctaa attcaaaacc
     7621 ggtaaggctc taaaagacgc tctaaaaaat aaacgctaag aattgctttt taaaaaaata
     7681 tcggttaaaa tccgaattgg ctcccgtggt gaaatggata tcacgcttgg cttcggacca
     7741 agtattttgg gttc
//
LOCUS       DAOPAI010000025         7428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_9001_length_7428_cov_5.077173, whole genome shotgun sequence.
ACCESSION   DAOPAI010000025 DAOPAI010000000
VERSION     DAOPAI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9001_length_7428_cov_5.077173"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1180)
                     /locus_tag="PKV49_03045"
     CDS             complement(95..1180)
                     /locus_tag="PKV49_03045"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPL22616.1"
                     /translation="MFKSLLSVRYLVLGFVLVSLLGGCSISIGRGPNQLGSPDVGGVF
                     RSGNRGDNWQSADLIPTNTGRPNSISTLNVNMLALDPNDYDALYFASQDNGLIFSYDG
                     GQNWQIANSLGRINVSSVAIDYHDKCTIFAASANRLLRSDDCSRSWSQAYHDNDPRIA
                     ITSLLTDHHNAGFVYLGTSRGDVLRSGDNGNSWQTVKRFDNPIRKIIMSPRDSRVMFV
                     ATENSGLFRTMDGGGSWENLNQTLAEFEIGNQFRDLVVSLEDPSIIYLATDYGLFKSI
                     NYGDAWTRINLLTPEQSTIINALAINVNNSQEIYYVTDTTFYRSIDGGVNWSTKKLPT
                     SRSGWSIIINPRQPEVVYLAVKAIKRK"
     gene            complement(1188..2210)
                     /locus_tag="PKV49_03050"
     CDS             complement(1188..2210)
                     /locus_tag="PKV49_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22617.1"
                     /translation="MEIQGHQLHWSHVGNERVVRCWAAHLASRQLAQSYLLIGPNDIG
                     KATVAKNFARTLVCQRRDQPGVDWPCEQCSACRQTAAGIYPDFYEIDLLPDKKMISIE
                     QIRQLLEKISLSSFADNYQVAIIRRAEALSDSAANALLKSLEEPNPSVIFILMTDSLS
                     SVPGTIASRSQIFRFYPVSADLIYDYLVNEWGVNRELAKNLAQVCLGRPNLAIRFFEQ
                     PDLFTAYCQMAEVLLKLIASQSLSERLLSLQQIYPKANSTAGMAEWQLWQLWLGLARD
                     LLLIKQGQLPLIQHWFLRERLVATAEQITWLNLLTIFTNLGAARQYLLANVQPNLVFH
                     NLAAGN"
     gene            complement(2353..6933)
                     /locus_tag="PKV49_03055"
     CDS             complement(2353..6933)
                     /locus_tag="PKV49_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPL22618.1"
                     /translation="MSVKFKSYRLFALLLLIIPLPLQAYDHSVTHPRLTDWSVQLYNQ
                     KYSLPISDQQKHWLMLGAAAEDQPLLRTMNHFWWPQTNRPLSAGACQLMSLHTAPSWG
                     LDGRAQASFIYGSIFSWNQAILAYRSGDYQQAFINLGHVLHLLQDSSVPAHVRNDPHE
                     LGDSLENWVWQHRQQLTQPPAVVHPNCLSYHDCFRQLASAVNQHYFSQDTINSPMESG
                     DWPSQPTVAAWPDNDGYIIYRGYKLAYYQSARRRLILDDQVQADYWSQLAPLAIGYGT
                     ELLRVFLSIANSQAEIVAEPNWWSRWLATIKTNPIHQSVTILSSPAANSSTKPVNNID
                     NSASSQPVMATTASPLVKRVIDGDTIELADGQKVRYIGIDAPELAQTSGQASQCLANE
                     AKQRNQALLAGGVLRLVADSGGDKDAYGRLLRYVYVGDLFLNQQLAAEGLVKVFFCGP
                     NQDNCPPATDQARRQLITAAGQAAKQAQLGIYGRQCLVTANTSTATTTNSKTKTASST
                     NKTTTTTSTVTRSTAKTTSTVKTTASSTSNQMTLTTNSLTNSKNTSSTAIKPTSTDST
                     PPDTTIQTTLPAITNTGTANFLLSSTEPGKFECQLNDQNWKTCPADYQLKNLVSGEYL
                     LSVRAIDLAGNIDPTPATYTWLIDKKKPSISWLTKPSSTVAGWEVEFSVETSEEVALS
                     CQLDNQPIQSCQTSFSYEQLTVGQHQIVVQAVDLAGNYSAKLTHKWRMLGGAPDGLSI
                     DYPPANPWWVSSTPITISGQAGQASRVLIGSQVAKLSANHTWQASIKLVPGLNNLVIW
                     PENEAGQAGASSTIAIILDNIKPSSAVSVLPETITELGFIVEWQGTDSGDLASGQLVY
                     DVQYRLDNHSWQPWLSAVDYTSAVFDQPAEINQKIAFRCRARDRVGNWENWPASSAAD
                     AQTKLVAVPSGPPPKVVISQLATRGPAGAGDEFVELYNPNDQAVDLSAWRLQSKSASG
                     ATWINRTGDGLPTGSSIAAHGYFLLASADYSGEIMPDYRHGGGWGLSDDGGHLRLIDS
                     HEYLLDKLGYNQANDPEGQPASGDLAQEHSLQRKARAESTAASLAPGGAEAGLGNGWD
                     SDNNQADFVDQSVVQPRSSSQQVIDSSAISTGLAHLWHFSECLGTTLYDYVGSADINN
                     QPFGWRVGPFDCAGYQTWEQPAVNLDLTVPLDPSQVTLAYWWRNASYPNEGRGHEYLQ
                     AADGQIILGLTPSASGVLQFWHYGANYRLENIIPTDDNWHLLAATQNQNQLKFYVDGR
                     LKFQDAAEYNPPQPIVKLDLADENYPIDRDEIAIWQRALSLAEIRQLYQSNQPLNPYP
                     VRPIQSEPVAAYYWSFDEGVSNIAYDSLAQVQLAPVARWIYSPFNLGVRLEHPGDLQG
                     DLPQPLENRDVSLSFWWRSSAYPNESRANIILASQSGQPLFGLGIGTYGPKIHFNGAS
                     WYPLNLPINDGDWHQAVLVYDSYAYRLSFYLDGRRRWHSEQTWLISPLTKLLAAQDNW
                     PLDIDELTIWQGALNANQIETIYELAKPELDK"
     gene            complement(6935..7417)
                     /locus_tag="PKV49_03060"
     CDS             complement(6935..7417)
                     /locus_tag="PKV49_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HPL22619.1"
                     /translation="MWKKIFTRPYIWWLVAAAAVIFLLIFAALVWPAIQRHLYQQRWL
                     QPFINDAKNSLGGDSPQAAYQQFWQALQNNQRETALAYIIGLRRDYYQRVWQDQQRWQ
                     LDQSLPQLVSLVFQGDCLPEAVACRQRAILSYTTDHGRDGRLELYQNLAGRWQIGDIY
                     "
BASE COUNT         2261 a         1755 c         1550 g         1862 t
ORIGIN      
        1 taaaaaatgg cgttgacact gctccgtgag ccgttgttaa aaccccaagc cgcgcggctg
       61 atatggctga ttgcttatcc agctgaaaca tggactattt gcgtttgata gctttaaccg
      121 ctaaataaac aacctccggc tgtcgtggat tgataataat agaccaacct gaccgagagg
      181 tcggcaattt tttagttgac caattaactc caccatcaat cgaacgataa aaggtggtat
      241 cagtaacata ataaatctct tgactattat taacattaat agctaaagca ttgataatag
      301 ttgactgttc tggcgttaac aaattaatgc gcgtccaagc gtcaccataa ttaattgatt
      361 tgaataaacc ataatcagtg gccagataaa taatcgacgg atcctccaag gaaacaacta
      421 agtcgcgaaa ttgattgccg atttcaaatt cagccaaggt ttgatttaaa ttttcccagc
      481 taccgccgcc gtccatcgtt ctaaataaac cgctgttttc cgtagcgacg aacataactc
      541 ggctgtcgcg cgggctcata ataatcttgc gaatcggatt atcaaaacgc ttaaccgtct
      601 gccagctatt gccattatca ccgctacgca aaacatcgcc gcgtgaagta cctaaataaa
      661 caaaaccggc attatgatgg tcagtcagaa gactggtaat ggcaatacgc ggatcattat
      721 catgataggc ttgcgaccaa ctacggctgc aatcatcaga acgcaacaac cgattggccg
      781 aagcggcaaa aatagtgcat ttgtcatgat aatcaatcgc caccgaacta acattaatcc
      841 gtcccaaact attagctatc tgccaatttt gaccaccatc ataactaaat atcaaaccat
      901 tatcttggct ggcaaaataa agagcatcgt aatcattcgg gtcaagcgcc aacatattaa
      961 cgttcagagt actgatactg ttgggacgac cagtattggt tgggataaga tcggctgatt
     1021 gccaattgtc gccgcgatta ccgctacgaa aaacgccgcc aacatcagga gaaccaagct
     1081 gattaggacc acgaccaata ctgattgaac aaccgccgag caggctgacc aaaacaaatc
     1141 ccagaactaa ataacgaact gataataaag atttaaacat aaagcgatta gttgccggcc
     1201 gccagattat ggaaaaccaa gtttggttga acattggcta ataaatactg gcgagcagcg
     1261 cctagattag taaatatagt taataaattg agccaagtta tttgttcggc cgtcgcgact
     1321 agtcgctcgc gcaggaacca gtgttgaatt aacggcaact gcccttgctt aatcaataat
     1381 aaatcacgcg ccagacccag ccaaagctgc caaagctgcc actcggccat accggccgta
     1441 gagttggctt ttggataaat ttgctgcaaa ctaagtaaac gctcggacaa tgattggctt
     1501 gcaattaatt ttaatagaac ttcggccatc tgacaataag ccgtaaataa atcaggttgt
     1561 tcaaaaaaac gaatagctaa attagggcgg cctagacaaa cttgggctaa atttttagct
     1621 aactctcgat tgacccccca ttcattgact aaataatcat aaattaaatc agccgacact
     1681 ggataaaaac gaaaaatctg actgcgtgac gcaatagtgc ccggcacaga agataaacta
     1741 tcagtcatta agataaaaat aacactggga ttgggttctt ccaggctttt taagagcgcg
     1801 ttagcggctg aatcgcttaa agcctcagcg cgacgaataa tcgccacctg ataattatcg
     1861 gcaaaagacg aaagactaat cttttctaat aactggcgaa tctgttcaat gctgatcatt
     1921 tttttgtccg gcaataaatc aatttcataa aaatccggat aaataccggc agccgtttga
     1981 cgacaagccg aacactgttc acaaggccag tcaacaccag gttgatcacg gcgctgacac
     2041 accaaagtgc gggcaaaatt tttagcaaca gtcgctttac caatatcgtt tggaccaatc
     2101 aaaagatagc tctgcgccaa ttggcgagaa gccaaatgag ccgcccaaca acgaacaact
     2161 cgttcattgc cgacatgcga ccaatgcagc tgatggccct gaatttccat actattatta
     2221 gattatcacg gcgagagcag attggcaaca aaaattaatt ttttgatgaa tatcgacaac
     2281 ttagaaaaac tcttacttaa aagaaaagac cgctattaaa tatctgccca taataaacta
     2341 taaacaaata aactatttat ccagctccgg tttagccagt tcatagatag tttctatctg
     2401 gttggcatta agagcgccct gccaaatagt taattcatca atatctagcg gccaattatc
     2461 ttgggcagcc aataatttag ttaatggact aataagccaa gtctgctcgc tgtgccaacg
     2521 ccgccggcca tctaaataga atgataaacg ataagcataa gaatcataaa ctaaaaccgc
     2581 ttgatgccaa tcgccatcat taatcggtaa attaagcgga taccagctgg ctccattgaa
     2641 atggatcttc ggtccataag tgccaatacc taaaccaaac aacggctggc cggactggct
     2701 agctaaaata atattagccc gactttcatt gggataagca ctactacgcc accaaaaaga
     2761 caaagaaaca tcacgattct caagcggctg tggcaagtcg ccttgtaaat caccaggatg
     2821 ctccaaacga actcctaaat taaaaggact ataaatccaa cgggccactg gcgctaattg
     2881 gacttgagct aaactatcat aggcaatatt ggaaacccct tcatcaaagc tccaataata
     2941 agctgcaacc ggctctgatt ggattggcct tactggatat ggattaagcg gctgattaga
     3001 ctgataaagc tgccggattt cagctaggct taaggctcgt tgccagatag caatttcatc
     3061 acgatcaatc ggataattct catcagctaa gtccaattta acaatcggct gtggcggatt
     3121 gtattcagcc gcgtcttgaa acttcaaacg gccgtcaaca taaaatttta attgattttg
     3181 attttgagta gccgccaata aatgccaatt atcatcagtg gggataatat tttccaagcg
     3241 ataattagcg ccatagtgcc aaaattgcag cacccccgaa gctgagggag tcagtcccaa
     3301 aataatttga ccatcagccg cttgcaaata ttcatgacca cggccttcat taggataaga
     3361 ggcattgcgc caccaataag ctaaagtcac ttgactggga tcaagcggca ctgttaaatc
     3421 aagattaacc gccggctgct cccaagtttg atagccggca caatcaaacg gaccaactcg
     3481 ccaaccaaaa ggctgattat taatatcagc cgaaccaaca tagtcataaa gagtcgtccc
     3541 caaacactca gaaaaatgcc acagatgggc caatccagta ctaatagcgc tcgagtcaat
     3601 aacttgctgg ctactagatc gcggctgaac tactgactga tcaacaaaat cggcttgatt
     3661 attatcacta tcccagccat tgccaagacc ggcctcggcc ccgcccggag ctaagctagc
     3721 ggcggttgat tcagcccgtg ccttgcgctg taaactgtgc tcctgagcta aatcaccgct
     3781 agccggttga ccttcaggat cattggcttg attataaccg agtttatcta ataaatattc
     3841 gtgactatca atcaaacgca aatgtccgcc atcgtcagac agcccccagc cgccaccatg
     3901 gcgataatca ggcattattt ccccggaata gtcagcgcta gccaatagaa aataaccgtg
     3961 agcggcaata gaactaccgg ttggcaaacc atcgccagtc cgattaatcc aagtggcgcc
     4021 actggctgat ttgctttgca aacgccaagc gctcaaatca accgcttgat cattagggtt
     4081 atataattca acaaattcat cgccggcacc agccggtccg cgcgtggcca gttggctaat
     4141 aacaacttta ggcggcggac cgctgggaac ggccactaat ttagtttggg catcggccgc
     4201 cgagctggcc ggccaatttt cccaattgcc tacccgatca cgagcccgac aacgaaaagc
     4261 aattttttga ttaatttctg ctggttggtc aaaaacagcg ctggtataat caacggcact
     4321 aagccatggc tgccaagaat ggttatctaa acgatattgg acatcataaa ctaactggcc
     4381 gctagccaaa tcaccactat ccgttccttg ccactccact ataaaaccaa gctcagtaat
     4441 agtttctggt aaaaccgaaa ccgctgaact gggttttata ttgtctaaaa taatagcgat
     4501 ggtgctgcta gccccagcct ggccggcttc attttccggc caaattacta aattattcaa
     4561 tccgggcact aatttgattg aggcctgcca ggtgtgatta gctgagagct tagccacttg
     4621 gctgccaatt aaaactcgac tggcttgacc agcttgacca ctaatggtaa taggcgttga
     4681 gctgacccac cacggattgg ccggcggata atcaatagat aatccatccg gagctccgcc
     4741 aagcattctc catttatggg ttaatttagc tgaataattg ccggccagat cgactgcctg
     4801 aacaacgatt tgatgctggc cgacagtcaa ttgctcataa ctaaaactgg tttggcaaga
     4861 ttgaatcggc tgattatcta attgacaaga aagcgcgact tcttcactag tctccactga
     4921 aaattcaact tcccagccag ccacagtgct ggacggctta gtcaaccagc taatgctggg
     4981 ctttttcttg tcaatcagcc aagtgtaagt cgccggagtc ggatcaatat taccagccaa
     5041 atcaatggcc cgaactgaca ataaatattc cccgctaacc aaattcttta attgataatc
     5101 ggccggacaa gttttccaat tctggtcatt gagctggcat tcaaatttac caggctcagt
     5161 ggacgataat aaaaaattag ccgtgccggt attagtgatg gctggcaaag tagtttgaat
     5221 agtggtgtct ggtggtgttg aatcggtgct ggttggttta atagccgttg atgaagtatt
     5281 tttagaatta gttaacgaat tagtggttaa agtcatctga ttactcgtag aactggcagt
     5341 agttttaact gttgaggtag ttttagcggt tgaacgagta accgtactgg ttgtggtggt
     5401 agttttgttg gttgaactag ctgttttggt cttagaatta gttgtcgtag cggtagaagt
     5461 attagcggtc actaagcatt gacgaccata aatacctaat tgagcttgtt tggctgcttg
     5521 gccggccgcc gtaattaatt gacggcgagc ttggtccgtg gctggtggac aattatcttg
     5581 attggggccg cagaaaaaaa ccttaaccaa accttcggcg gccaattgtt gattgagaaa
     5641 aagatcgcca acataaacat agcgcaataa acggccataa gcatccttgt ccccaccgct
     5701 atcggccact aatctcaaaa ctccgccagc taataaagcc tgattgcgtt gtttggcctc
     5761 attagccaag cactgactgg cttgtccgga agtttgagct aattccggcg catcaatgcc
     5821 aatataacga accttctgac catcagctaa ttcaatagta tcgccatcaa taacccgttt
     5881 aaccagcgga ctggcggtag tcgccatgac cggttgacta ctagctgaat tatcaatatt
     5941 attgaccggc tttgttgaag aattagccgc tggcgacgac aatattgtca ccgactgatg
     6001 aatcggattg gttttaatag tggctaacca acgcgaccac caattgggct cagccacaat
     6061 ttctgcctga ctattggcga ttgatagaaa aacccttaat aattcagtac catagccaat
     6121 ggctaaaggt gctagctgcg accaataatc agcttgaacc tgatcatcca aaattaaccg
     6181 acggcgcgct gattgataat aagccaactt atagccgcga tagatgatat agccatcatt
     6241 atcgggccaa gcggccacgg ttggttggct gggccaatcg ccggattcca tcggactatt
     6301 aatggtatct tgactaaaat aatgctgatt aacagcgctg gctaattgtc gaaaacaatc
     6361 atgataacta agacaattag gatgaaccac ggctggcggt tgagttaatt gctggcggtg
     6421 ttgccaaacc caattttcca aagaatcgcc taattcgtgt ggatcattac gcacatgagc
     6481 tggaacgcta ctatcttgca aaagatgcaa aacatgccct aagttaataa atgcctgctg
     6541 ataatctccc gaccgataag ctaaaattgc ctgattccaa ctaaaaattg aaccataaat
     6601 aaaactagct tgcgcgcgac cgtccaagcc ccaactaggt gctgtgtgca atgacatcaa
     6661 ttgacaagcg ccggcgctaa gcggccgatt agtttgcggc caccaaaaat ggttcattgt
     6721 tcgcaataac ggctgatctt cagcggccgc ccccagcatt aaccaatgct tctgctgatc
     6781 actaatcggc aacgaatatt tttgattata tagctgaact gaccaatcag tcagccgtgg
     6841 atgagtaacg ctatggtcat aggcttgcag cggtagcgga ataattaaca gcaacaaggc
     6901 gaacagccga tagctcttaa acttgactga catattagta aatatcacca atttgccagc
     6961 gaccggctaa attttgatac agctctaacc gtccatcacg accatgatca gtggtgtagc
     7021 tcaatattgc ccgttgtcga caagccacgg cctcaggcag acaatctccc tgaaaaacta
     7081 aactaactag ttgcggcagt gattgatcta actgccaccg ttgttggtct tgccagactc
     7141 gctgataata atcacgacgc aaaccgataa tataagctaa ggcggtttcg cgctgattgt
     7201 tctgcagagc ttgccaaaat tgctggtaag ccgcctgcgg actatcaccg cccagactat
     7261 ttttggcatc attaataaat ggctgaagcc atctttgttg ataaagatga cgctgaatag
     7321 ctggccaaac taaggcggca aaaatcaata gaaaaatcac cgcagcggcg gccactaacc
     7381 accagatata gggtctggtg aatatttttt tccacataac tcactatg
//
LOCUS       DAOPAI010000026         6851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_9879_length_6851_cov_3.884638, whole genome shotgun sequence.
ACCESSION   DAOPAI010000026 DAOPAI010000000
VERSION     DAOPAI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9879_length_6851_cov_3.884638"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PKV49_03065"
     CDS             complement(<1..1311)
                     /locus_tag="PKV49_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22620.1"
                     /translation="MNRISKVFVLGLAIVALAGFGKIGTTNAAAQPGSLIKMNGLSSV
                     YYLGADSKRYVFPNSTVYFSWYKDFSGVVTISQSELESYPLAANITMRPGTRLVKITT
                     NPKVYAVEPGGTLRAIPDEATAVALYGSNWNKRVSDVADAFFTNYKTGNALAANEVPA
                     GSLVKNAGNAAVYYFDGTNYRSIASEAAFNANRFSFDNVMTISNTITAGGTAITANEF
                     ANPDSSATGIGTIPGQGTGLTVALSASTPASQDVPQSVATTIMKFNVTAANDGDVNVT
                     GVKFTAIGLGDAQDIDQVSVFNNNNRLNNSARDVDSNKEAQINFSQPFTVAKGTTATF
                     EVRAKISGTAKYGLSINKAADIATTATVSGSFPVNGNIMSGVAVTVGQLTIAQDGSMS
                     DIKLGDKAAVIGKFKLSETSGVEDVTLNAISFKRDPDGTASDDDV"
     gene            complement(1687..2442)
                     /locus_tag="PKV49_03070"
     CDS             complement(1687..2442)
                     /locus_tag="PKV49_03070"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HPL22621.1"
                     /translation="MNDHFVSQQLKKLRQITPNADWVSQQRQVIMAQVYHGQQPASLA
                     FSRWSDFWLSLKYAFSQPGVVVSAILATVLLGGGTTSYLVGRQAKPGDSLYIAKQLSE
                     RAQLLAVSFDRAEQNKLKLALASQRVSEMAAVDQQADQQQAAAISREFKQTVSDVKDR
                     LAQAETKAAAAALVKASQPKTVASNKTEDTFSAATGKDKTGRLEVSQPVTATATKTPS
                     QIIEEAEQLFDKQEYSAAIDKLEAASQLMDSAK"
     gene            complement(2462..3031)
                     /locus_tag="PKV49_03075"
     CDS             complement(2462..3031)
                     /locus_tag="PKV49_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPL22622.1"
                     /translation="MTKSLVKQSSDRLLFLRLKIKDQAAFIQAYEAHQADIYRFIYFK
                     VNSEAEAQDLTSTVFLKVWSVVQAGKLKGYNTLKSLLYTVARNTVIDFYRDNGKLNKV
                     SLDNQDEPIDIVDDKPGAHELVQQDSDLQIIYRALGELKPEYREVIIMHFVNELSVAE
                     IAQVTGKKPGNVRVIIHRGLEALRKLANE"
     gene            3388..3696
                     /locus_tag="PKV49_03080"
     CDS             3388..3696
                     /locus_tag="PKV49_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPL22623.1"
                     /translation="MPRITAATDHTLKASPVTTPAMTNHTIVNPLWVSVSEAAKLGGV
                     QSKTIRRAIGGQLITYRIRGNRYLIDLGSLLAFLRSRTKLHNKLTKAGLGQYVSGWVE
                     "
     gene            complement(3768..4046)
                     /locus_tag="PKV49_03085"
     CDS             complement(3768..4046)
                     /locus_tag="PKV49_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPL22624.1"
                     /translation="MSMTKAQLVDGLAKNCNLSKKAVNDVLNEMSTMAYKEVKKNGEF
                     IVPGIGKLVKVQRKARMGRNPATGAQIKIAAKTVVKFRVAKAAKDAVA"
     gene            complement(4178..6223)
                     /locus_tag="PKV49_03090"
     CDS             complement(4178..6223)
                     /locus_tag="PKV49_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPL22625.1"
                     /translation="MNNKHWLGILLALAGSWLTVGAAQAATTISGNIISPNWTAANSP
                     YIVSENIEVAPGATLTIEAGTVVKFKKNKGITVRGTLKINGNNSNIVVLTSAEATTTR
                     GDWAGITFADESVDAEFDSNGNYIGGSIIKYAVFNHSQGLKLDNSHPYIVNSNFLNNT
                     TAITISGTAPVDTSLNAAVDNHKLLISRNNITNNTIGLIINRAFLAPPMIFDPSGLAA
                     NQIKANIVIDGNAINYNQEGIWLQQAQRAAILNNQLNFNNNYGLKISAGAPNAIVQHN
                     TINYNNIGLELAARGANIVKNAIAYNNQAGLNFLNTNNQINNNNIYGNLGYDLRSPLA
                     GGSLANNYWGVATPINASLPTSSSFTIEPRLIEPTDIASLATPTINDFATSTILDKIL
                     VSGTKDASADIWINGRQAVALDSEVTWSYSLPLEMGTNDFSLLSKDASGKVSPTVNFT
                     ISRLENKFVPVPTINSFKPTTDQTTQALSGSKDINTAIMINGQEAVALDNKTTWQATV
                     VLKYGRNDFTIVAQSAAGYQSDSLAISIQRPDTTGQQVLTAEAQASAGIATDTALTKK
                     MAGRILLQVEAKGEAWYVNPLNGQRYFLAGPANAFNLMRQLGLGVKHSVITAKIVDSK
                     LLGRILIDVDDSGKAYYVHPTTKKLYYLGRPADALQVMRQVGLGINNQNLRKIKLAQ"
     gene            complement(6264..>6851)
                     /locus_tag="PKV49_03095"
     CDS             complement(6264..>6851)
                     /locus_tag="PKV49_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22626.1"
                     /translation="QSAAGNSPDKLLAAVDARPRSRWRRFLDSLYRVVYSFKNIKLRR
                     LLVNRHLWLGVFGFLLISFSSQLIINFSSELLQRWGWPPLLVGVLIFSIGTNLPEITV
                     TMRALIKKAGALSISHLIGSAVSNVLIIGILLQFGRIPTKPALSFGLSAGTILVTSGL
                     FLIFYKTDKCFHRWEGVTLLAVYLIFLASQLIWAT"
BASE COUNT         1929 a         1586 c         1532 g         1804 t
ORIGIN      
        1 aacatcatca tcagaggctg taccatcagg atctctcttg aaagagatgg catttaaagt
       61 aacatcttcg acaccgctgg tttcagatag tttgaattta ccgataacag cagctttgtc
      121 gcctagttta atgtcagaca ttgaaccatc ttgagcaata gtcaattgac caacagtaac
      181 agcaacaccg ctcatgatat taccattaac cgggaatgaa ccagaaacag tagcggtagt
      241 agcaatatca gcagccttat tgatgcttaa accgtattta gcggttccag aaatcttagc
      301 gcgaacttca aaggtagcgg ttgtgccttt ggcaacagtg aaaggctgag agaaattgat
      361 ttgagcttct ttattgctat caacatcgcg agcagaattg ttcaaacgat tgttgttatt
      421 aaagacagaa acctgatcaa tatcttgagc atcacccaag ccaatagcag taaacttaac
      481 accagtaacg ttaacatcac cgtcattagc agcggtaaca ttaaatttca taatggtggt
      541 tgcaacgctc tgaggtacat cttggctagc aggagtgctg gcgctcaaag cgacagtcaa
      601 accagtgcct tgaccaggaa tagtgccgat accagtcgcg gaagaatccg gattggcgaa
      661 ttcgttggca gtgatagcgg tgccgccagc agtaatagta ttgctgatgg tcatgacgtt
      721 atcaaagcta aaacgattgg cattgaaagc ggcttcgcta gcaatactcc gataattggt
      781 gccatcaaaa taatagacag cggcattacc ggcatttttc accaaagagc cagctggcac
      841 ttcgttggca gctaaagcgt tgccagtctt gtaattggtg aagaaagcat cagcaacatc
      901 gctaacgcgt ttgttccaat tactgccata caaagcgacg gcggtagcct cgtcagggat
      961 ggcgcgcaag gttccacctg gttccacggc ataaaccttt ggattggtgg taatcttgac
     1021 taagcgggtg cccggtctca tggtgatgtt ggccgccaaa ggatatgact ccaattcact
     1081 ctgagaaatg gtcacaacac cgctaaaatc tttgtaccag gaaaaataaa cggttgagtt
     1141 tgggaagaca tagcgcttgc tgtcagcacc gaggtaataa acggaagata atccgttcat
     1201 cttgatcagg ctgcctggtt gagcggcggc attggtagta ccaattttgc cgaaaccagc
     1261 taaagcgaca atggctagac ccaaaacaaa aactttggaa attctattca tagtgtcgtt
     1321 tgtgtttggt aaatctttat catttaccag acgaattaat taatgataag ccttaaaagt
     1381 tggcttatct agtaaagcgc tgccaatgat taaaccgcgc acaacggttt agttggacga
     1441 gcgcgaactt tattgttaag tgccgggcag tttcctgacc attgacttag gcgtcaattc
     1501 gacctgcggt cctgccatct tagctttcag ctaagcctca cactcaacta ctctatcaag
     1561 cttacataga gtaattgccg cggaggatga aaccagccag gaggggtagg ctggcttcat
     1621 gctccgggca atcagcctaa tcttgataga attatatcaa tacaaggaac aactcaatta
     1681 aagcgcttat ttggcgctgt ccatcagctg gctggccgcc tctaacttat caatggcggc
     1741 ggaatactct tgcttatcaa ataattgctc agcctcttca ataatctggc tcggtgtctt
     1801 ggtagcggtg gcagtcaccg gctggctgac ttctaaccgg ccggtcttgt ctttgcccgt
     1861 ggcagcgcta aaagtatcct cagtcttgtt gctggccacc gtttttggct ggctagcctt
     1921 gactaaagcc gcggccgccg ccttggtttc agcctgggct aatctatctt tgacatcgct
     1981 gacagtttgt ttaaattctc ggctaatagc cgctgcctgc tgctgatcgg cttgctggtc
     2041 cacggccgcc atctcggaaa cgcgctggct ggccagagcc aattttaatt tattctgttc
     2101 ggcccgatca aaactgaccg ccaataactg ggcccgttca cttaattgct tggcaatata
     2161 taaggaatca cccggcttgg cctgacgacc aaccaaataa ctggtggtgc cgccgcccaa
     2221 cagaaccgtg gccaaaatgg cactaactac tactccgggc tgggaaaaag catatttcaa
     2281 agatagccaa aaatccgacc aacggctaaa cgctaagcta gccggctgct ggccgtgata
     2341 aacctgagcc ataatcacct ggcgctgctg gctgacccag tcggcattgg gagtgatttg
     2401 acgcagcttc tttaattgtt ggctgacaaa atgatcattc atatattcaa tctagaaaat
     2461 actactcgtt ggccagcttg cgcaaggcct ctaaaccgcg atggataatg acgcggacat
     2521 tgcccggttt cttgccggtt acttgggcga tttcagcgac tgataattca ttgacaaaat
     2581 gcattatgat aacctcgcga tattctggct ttagttcgcc caaggcccga tagataatct
     2641 gtagatcgct gtcctgctgg accagctcgt gggcgcccgg tttgtcatca acgatgtcaa
     2701 tcggctcgtc ctgattatcc aaggaaacct tgttaagctt gccgttgtcc cgataaaaat
     2761 caataacggt attgcgggcc accgtgtaaa gcaagctttt cagcgtatta taacctttta
     2821 atttaccggc ctgaacgact gaccaaactt tgagaaaaac agtggaagtc aggtcttggg
     2881 cttcggcctc ggaattgacc ttgaaataga tgaaacgata gatgtccgcc tgatgggcct
     2941 cataggcctg gataaaggcg gcttggtctt tgatcttgag gcgtaaaaat agcaggcggt
     3001 cgctggattg tttgactaaa gatttcgtca tgttcggcta cgattatata tacgataaaa
     3061 gtggataagt gttacagtca aatagcgcta ttcatttaac cataaagtcg gcagaaaaac
     3121 aagccaatca atgccatcgg ccaagggctt gctttttttt ggccagtggt ctacaataga
     3181 aaatccaatg ttttgtccag attagactag actttatttg acaaaaaagc aaaaatgttc
     3241 tttatttaag tctacataaa cctagactta aattctgcaa taatggctga tttcttatct
     3301 ttagtctaag tatatctaga ctaaagtcga aaaataacta aacaccgcca accacccttg
     3361 attaaagtct aactattcct agactatatg ccgagaatta ctgccgccac cgatcatact
     3421 ctcaaagcca gtccggtcac cacgccggcc atgactaacc acactatagt caatccgcta
     3481 tgggtatcgg tgtcggaagc ggctaagctg ggcggcgtcc aaagtaaaac cattcgccga
     3541 gccattggcg gccagctgat tacctaccgc attcgcggta atcgctactt aattgacctg
     3601 ggctcgctac tcgccttttt gcgttcgcgt actaaattgc acaacaaatt aaccaaagcc
     3661 ggtctagggc aatatgtgag cggctgggtt gaataagttc aataaataaa atcaccaacc
     3721 ctttatgggg gttggtgatt ttttgcaaag aaacttgagg caataaacta ggccacagcg
     3781 tctttggccg ccttggcgac gcggaattta accacggtct tggccgcgat cttgatctga
     3841 gcgccagtcg ctgggttgcg gcccatgcgg gctttgcgct ggaccttaac caatttacca
     3901 atgccaggaa cgatgaactc accattcttt ttgacctctt tataggccat ggtggacatt
     3961 tcgttcaaga catcgttgac ggctttcttg ctaaggttgc aattcttagc caaaccatca
     4021 accaattgag cctttgtcat agacataggt gtaatgattt attacctggc agtaaacctg
     4081 ccattcttat tgatattata tacctttact gaaaagctga caagtgttag tcaataaaga
     4141 caagggtcgg gctggaaaaa acaacgaatc gtcaatatta ttgggctaat ttgattttac
     4201 gtaggttctg attattaata cccaaaccga cctgacgcat cacctgcaaa gcatcggccg
     4261 ggcggcctaa gtaatataat ttcttggtgg tcggatgaac gtaataagct ttgccgctat
     4321 catcaacatc aatcaggatg cggcccaata atttactatc aactattttg gcggtaatga
     4381 cactgtgctt gacccccaaa cccagctggc gcatcaaatt gaaagcattc gccggaccgg
     4441 ccaagaaata ccgctggcca ttaagcggat tgacatacca agcctcgccc ttggcttcaa
     4501 cttgcaataa aatacggccg gccattttct tggtcaaagc agtatcggta gcaataccgg
     4561 ctgacgcttg cgcttcagcg gtcaaaacct gctggccagt cgtatccggc cgctggatgg
     4621 aaatagctag cgaatcgctt tgataaccgg cagcgctttg ggcaacgatg gtaaaatcat
     4681 tgcggccgta tttcaggaca actgtcgctt gccaagtagt cttattgtct agcgctaccg
     4741 cctcttggcc gttaatcata atggcggtat tgatgtcttt gctgccgctt agcgcctgag
     4801 tggtttgatc ggtggtcggc ttaaaactat taatagtcgg cacgggcaca aatttattct
     4861 ctagccggct gatggtgaag ttgacggttg gactgacctt gccggaagca tctttggata
     4921 gcaggctaaa atcattggtc cccatctcta ggggcagact atagctccag gtgacttcac
     4981 tgtccaaggc gaccgcctga cgaccattaa tccagatatc ggcgctagca tctttagtgc
     5041 cactaactaa aattttatcc aggatggtgg aggtggcaaa atcattaata gtcggcgtag
     5101 ccagactagc gatatcagtc ggttcgatca atctcggttc aatggtaaaa ctggagctgg
     5161 tcggcaaact ggcattgatg ggtgtggcca ctccccaata attattggcc aaagagccgc
     5221 cagccaaggg gctgcgcaga tcatagccca gattaccata aatattgtta ttattaatct
     5281 ggttattggt gttcagaaaa ttcaggccgg cttgattatt gtaagcaata gcattcttaa
     5341 cgatattggc gccacgagcc gccagctcta gaccaatatt attgtaatta atggtgttat
     5401 gctgaacaat ggcattaggg gcgccggcgc taatttttag cccataatta ttattaaagt
     5461 taagctgatt gtttaaaatc gccgcccgct gagcttgctg caaccaaatc ccctcttgat
     5521 tataattgat ggcattaccg tcaataacga tattagcctt aatctgatta gcggccagac
     5581 cgctgggatc aaaaatcatc ggcggggcta aaaaggcacg attaataatt aagccaatgg
     5641 tattattagt aatattatta cggctaatca acagcttgtg attatcaacg gccgcattaa
     5701 gggaggtatc aactggcgcc gttccactga ttgtaatggc cgttgtatta tttaaaaaat
     5761 tactgttgac aatataagga tgactattgt ccagcttgag accctgacta tggttaaaaa
     5821 cagcatattt aataatactg ccgccaatat agttaccgtt ggaatcaaac tcggcatcaa
     5881 ctgactcgtc ggcaaaagta atgccggccc aatcaccgcg cgtagtggtc gcttcagctg
     5941 aagttaaaac tactatatta ctattgttgc cattaatctt cagcgtgccg cgaacagtaa
     6001 tgcctttatt tttcttaaac ttaactactg ttcccgcctc aatcgtcaaa gttgcgcccg
     6061 gcgcaacttc aatattctcg ctaacaatat aagggctatt ggcggctgtc cagttggggc
     6121 taataatatt gccggagata gtggtggccg cctgagccgc tccgaccgtt agccagctgc
     6181 cggctaaagc cagcaaaata cctaaccaat gcttgttgtt cataaaaata aaattagtaa
     6241 atctatttta gcttataatt aaattaagtc gcccaaatta attgactagc taaaaatatc
     6301 aagtaaaccg ccaataaagt aaccccttcc cagcgatgaa aacatttgtc cgttttataa
     6361 aaaattaaga aaagaccgct agtcactaaa atggtcccgg cggacaagcc aaaagatagg
     6421 gctggtttag tcggaatacg gccgaattgc aacaggatac cgataatcaa cacattgctg
     6481 accgccgagc cgattaaatg gctaattgat aaggcgccgg cttttttaat taacgcccgc
     6541 atggtaacgg taatctctgg caaattagta ccgatagaaa aaatcaatac gccaactaac
     6601 agcggcggcc aaccccagcg ttgtaataat tcagaggaaa aattaataat cagctggctg
     6661 gaaaaagaaa ttaataaaaa accaaagact cccagccata aatgacgatt gactaataaa
     6721 cgcctaagct tgatattttt aaaactataa acaactcgat acagactatc taaaaagcgc
     6781 cgccaccgac tgcgcggccg agcatcaaca gccgctaata atttatcggg cgaattgcca
     6841 gccgctgatt g
//
LOCUS       DAOPAI010000027         6764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_10040_length_6764_cov_5.286183, whole genome shotgun sequence.
ACCESSION   DAOPAI010000027 DAOPAI010000000
VERSION     DAOPAI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10040_length_6764_cov_5.286183"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PKV49_03100"
     CDS             <1..511
                     /locus_tag="PKV49_03100"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPL22627.1"
                     /translation="LAALDFDGYNIGGLSVGETTAEMYQVLDWLTPAMPNDKTRYLMG
                     VGYPDNIVEAVRRGVDMFDCVIPTREGRHGRLFFWQADRSLVDYQGRVRDFYRTENIK
                     NSQFAELDQPINPHSRLPILRRYSLAYLHYLYKIDEALGRRLASLHNLEFYYDLMAAI
                     RQAIAAGQL"
     gene            517..1260
                     /locus_tag="PKV49_03105"
     CDS             517..1260
                     /locus_tag="PKV49_03105"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPL22628.1"
                     /translation="MEIIALSTRLLLPPQDDALTAITTALDQAGGLHNGDILAITSKV
                     VSLHQGRVIPIDQVPDKDQLIINQADSYLPRQLVPHNYAVLTIHNHLIAPSAGIDESN
                     AQEYYVLLPDNPEQLAKDWRQQLIRHYKLQQLGLIITDSRSMPLRYGVVGVGLAFAGF
                     QPLRDYRGQPDLFGRPMKISQTNIVDSLAAAAVYAMGECAEQTPIAIIRDIAGVQFTG
                     RDCANQLWLNTEDDIYQPLYQNFLPGGRS"
     gene            1266..2360
                     /gene="mnmA"
                     /locus_tag="PKV49_03110"
     CDS             1266..2360
                     /gene="mnmA"
                     /locus_tag="PKV49_03110"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPL22629.1"
                     /translation="MANSVKKQKILVAVSGGVDSAVAAQLLKNQGHEVIGVFMQFWKD
                     TSVDSATNNCCSLESWSDAQMVCQKIGIPLYALNFAQEFKRRVVNDFLAAYQSGRTPN
                     PCVLCNKQVKLGRLVKYAQKIGFDAVASGHYVRRLIDGTNIQLWQAKDLSKDQSYFLY
                     QLDQRDLAMLQFPLGDFHKTDIRRLAEKFHLPVADKAESQEVCFVSDSLPNFLRRHLQ
                     FTSGPIKDQHGRRLGQHQGLPLYTIGQRRGVELGGDGPYYVTGIDIATNTLLVTNQPN
                     DQRLFQDKIFLPRLYWLDRQRPRRSLVCQVVIRYHHPPVDCQLSRYQGGWLVELASRQ
                     RAVTPGQSAVFYHHGRLLGGGVIALDRGFC"
     gene            2406..4748
                     /locus_tag="PKV49_03115"
     CDS             2406..4748
                     /locus_tag="PKV49_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22630.1"
                     /translation="MKPARKASRFWRRPSRSSWPETIIQVVISAICLLLPGLFINFSW
                     QGANLDRLYLLALLVIIGLAAWLAKVLSAGSLSWRWRRFDWLAIASLLAVSLATIWSP
                     AWRNSLFGGYGQPMRSLVFFWLLFFLYLLVVNNWSTKLRQWCWLAILISFSLIAIYSV
                     CQLFGLFIIPLAFTKTVGFNPIGSLSNLALFLAAALPLFLLAIDHSDDFVPAPSHKTR
                     LIWNIWLGLAIVATLVALAALGSFTIWPVVILGLLIVLIFGLSRLVKLTRCQLIATVG
                     ALAISFVLLVLGNLGWLKLALPSEVSLSRDFSWQIAKASLAHHPILGTGLASFNSAFS
                     RFKTVDFNAAVLWNLDFDLPASWLFESLVVVGGLGTLLVLTGLIWGLIIAWRRLLTVK
                     TDLEFEDVNFGVGLLAASMVLLVGGALLPVGNSLLLIFGLLWILLMVVTYQRLRRLPT
                     WSWRQAGQHSGAIWTAGVIIVVIALLSSLTYLSKIYLADIIAGRAVKNSSTDQQISGV
                     AAAQRLAPWREMYGFSLAQLSWVKANQIAEAAAGATSTEALAAQNQAQAYAQQAKQFL
                     DQNAKLIQNQPAGLKTLAALYEMIGDFDGSMATQQRLIAIDPNNPWPQAKLAQLKVVM
                     AYQASTKEDKDKLVEEALSGYQRALTLKPGWAEAYFYRANLYQAIQKFSEATDDLVQA
                     VNYSNGSPDYQLALAQLLNERAKVESDKSADFRLQAEQLAKGILASDSNNINALYVLA
                     IIYRDSGQTDLARQTVQDLLSKAQDSDKSIIQQQFADLLP"
     gene            4761..6530
                     /locus_tag="PKV49_03120"
     CDS             4761..6530
                     /locus_tag="PKV49_03120"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPL22631.1"
                     /translation="MTANQLRQKFLDFFASKQHAIIPSASLVPDNDPTVLFNTAGMQP
                     LVPYLLGEAHPLGRRLANAQKCLRTDDIDEVGDTTHLTFFEMLGNWSLGDYFQQESIT
                     WSWEFLTSPQWLNLPPDRLAATVFIGDQHAPFDQEAYQLWQEVGLPAERLAKLGKKDN
                     WWGPAGQTGPCGTDTEIFYWTGSEPAPAAYNPSDKRWVEIWNNVFMSYNKQADGSFQP
                     LAQRNVDTGLGLERVLATLNNQPDIYLNDIFQPAIAYLEQVSGLSYTNNPRPLRIIAD
                     HLRASIFLLADEKAITPSNKDQGYVLRRLIRRAVRLAHNWPRSTDEVLVGVAESIIQS
                     MGSAYPELVAKHDFIVNELKKEVTKFQVTLNRGLKELQRLIETGQPISGAETFMLYQS
                     YGFPLEMTKEIARESGRQVDEVGFQSELVKHQELSRTAAAGKFKGGLADDSVATTRLH
                     TAAHLLLQALRQVLGTDVYQRGSNITTERLRFDFSYPDKMTAEQISQVEAIVNQQIAL
                     ELPVACQEVSLDEARCQGAMGIFDAKYGERVKVYTIGSESPFSQEICGGPHVHNTAEL
                     GHFRIIKEESSSAGVRRIKAILE"
     gene            6534..>6764
                     /locus_tag="PKV49_03125"
     CDS             6534..>6764
                     /locus_tag="PKV49_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPL22632.1"
                     /translation="MDKLTILITGGAGYIGSHAVKLFLSRGYRVVVFDNLSRGWRQPL
                     DILAPLGDLVFVRGDLTKTADLDQLFANYNFAG"
BASE COUNT         1607 a         1340 c         1601 g         2216 t
ORIGIN      
        1 gttagcggct cttgattttg acggttacaa tattggtggt ttatcggtgg gtgaaacaac
       61 tgcagaaatg tatcaggttt tagattggtt aacaccagcg atgcccaatg ataaaacccg
      121 ctatttgatg ggagttggtt atccagacaa tatcgttgaa gcggtcaggc gtggagtgga
      181 tatgtttgat tgtgttattc cgacccgtga gggccgccat ggtcgtttgt ttttttggca
      241 agctgatcga tctttagtgg actatcaggg tcgagtgaga gatttttatc gcacggaaaa
      301 tattaaaaat agtcaatttg ctgagctgga tcaacccatt aatcctcatt cacgtttgcc
      361 gattttacgt cgttacagct tagcttattt gcattattta tataagattg atgaagcgct
      421 gggccggcgc ttggccagtc ttcataattt agaattttat tatgatttaa tggctgctat
      481 ccgccaagcg attgcggccg gacaattata atcactatgg aaataatcgc gctgtccacc
      541 cgtttattac taccgcccca agatgatgcc ctgacggcta tcacaactgc cttagaccaa
      601 gctggcggtt tgcataatgg cgatatttta gccatcactt ctaaagtggt tagtttacac
      661 cagggccgtg ttatacctat tgatcaagtt ccggataaag atcagctaat tattaatcag
      721 gctgacagtt atctgccgcg ccagcttgtt ccgcacaatt atgccgtgct cactatccat
      781 aatcatttaa ttgcgccatc ggccggtatt gatgaaagta atgctcagga atattatgtt
      841 ttattgccgg ataatcctga gcaattagcc aaggattggc gtcaacagtt gattcgtcat
      901 tataaactac aacagctggg cctaattatt accgatagtc gatcaatgcc gctacgttat
      961 ggtgtagttg gtgttggctt agcttttgcc ggttttcagc cgttgcgtga ttatcgcggt
     1021 cagcctgatt tattcggtcg gccaatgaag atttctcaaa ctaatatcgt tgattctttg
     1081 gccgccgccg ccgtttatgc tatgggtgaa tgcgccgaac agactccgat tgccataatt
     1141 cgtgatatcg ccggcgtgca atttaccggt cgtgattgtg ctaatcagct atggctcaat
     1201 accgaagatg atatttatca gccactctat caaaattttt tgccgggcgg ccgttcataa
     1261 cgactatggc taattctgtt aaaaaacaaa aaatcttagt ggctgtatcc ggtggtgttg
     1321 attcagcggt cgcagctcaa ttattaaaaa atcaaggcca tgaagtgatt ggtgttttta
     1381 tgcaattttg gaaagacacc agcgttgatt cagcgaccaa taactgttgt tctttggaat
     1441 catggtcgga cgctcagatg gtttgccaaa agatcggtat tcctttatat gctcttaatt
     1501 ttgctcaaga atttaagcgg cgtgtggtta atgatttttt ggctgcttat caatccggcc
     1561 gcactcctaa tccttgcgtt ttatgcaata agcaggttaa acttggtcgt ttagtgaaat
     1621 atgctcaaaa aattggtttt gatgctgttg ccagcggtca ctatgtgcgc cgcttgattg
     1681 atggtactaa tatccagtta tggcaagcta aagatttatc caaagatcag tcttattttc
     1741 tttatcaatt ggatcagcgt gatttggcta tgttgcaatt tccgcttggt gattttcaca
     1801 aaacagacat tcgccgttta gcggaaaaat ttcatttgcc agtggctgat aaggctgaaa
     1861 gtcaggaagt ttgttttgtt agcgatagtt tacccaattt tttacgacgc cacctccaat
     1921 ttaccagcgg gccgattaaa gatcagcatg gccgtcgcct tggccagcat cagggtttgc
     1981 cgctttacac gattggtcag cgtcgcggtg ttgaacttgg tggtgatggt ccttattatg
     2041 tcacgggtat tgatatagcc actaatactt tattggttac taatcaacct aatgatcagc
     2101 gtctttttca agataaaata tttttacccc gcctctattg gcttgatcgt caacgaccac
     2161 ggcggtcgct agtttgccaa gtagtaattc gttatcacca tccgccggtt gattgtcaat
     2221 tatctcgtta ccagggcggt tggttggtgg agttagctag tcggcaacga gcggttactc
     2281 ctggacagag tgcggttttt tatcatcacg gtcgattatt gggcggcggt gttattgctt
     2341 tagaccgtgg tttttgttaa actatgacta agtttattaa gttttagatt aaataagaca
     2401 agtttatgaa accagctcgc aaagcctcgc gtttttggcg gcggccatct cggtcttctt
     2461 ggccggagac tattattcag gtagttatat cggccatctg tttattgttg cccggattat
     2521 ttattaattt tagctggcag ggcgccaatt tagatcgtct ttatttatta gctctgttag
     2581 ttattattgg tttagccgct tggctcgcca aggttttgtc ggccggttct ttgagttggc
     2641 gctggcgtcg ttttgattgg ttagctattg cctccttgtt agccgtttct ttagcgacta
     2701 tttggagtcc agcttggcgt aatagtttat ttggtggtta tggtcaaccg atgcgtagct
     2761 tagttttttt ctggttgctg ttcttcttgt atttattagt agtgaataat tggtcaacta
     2821 agcttcgtca atggtgttgg ctggccatct taattagctt tagcttgatt gctatttatt
     2881 cagtttgcca gttatttggt ttattcatta ttcctttggc ttttactaaa acggtcggtt
     2941 tcaatccaat tggttcatta tctaatctgg ctttattttt agcagcggcc ttgccattat
     3001 ttttgttagc tattgatcac agtgacgact ttgttcctgc tccgagccac aaaacacgat
     3061 taatttggaa tatttggcta ggtctagcca ttgtcgccac tttagttgct ttggccgcct
     3121 tgggttcttt tactatttgg cccgttgtta ttttaggcct tttgattgtt ttgatttttg
     3181 gccttagccg tttagttaaa ttaactcgct gccaattaat agctaccgtt ggcgccttgg
     3241 ccatcagttt tgttttattg gttttgggta atttgggttg gttgaaatta gctttgccga
     3301 gcgaggtcag tttatcgcgc gatttttctt ggcagattgc caaggctagc ttggctcatc
     3361 atccgatttt aggtaccggg ttagctagtt ttaatagcgc ctttagtcgt tttaagacag
     3421 ttgatttcaa tgctgccgtt ttgtggaact tagactttga tttaccggcc agttggctct
     3481 ttgaaagctt agtggtggtt ggtggtttgg gaactttatt ggtgttgacc ggattaattt
     3541 gggggctaat tatagcctgg cgtcgacttt taacggttaa gactgattta gaatttgaag
     3601 atgttaattt tggtgttggt ctcttggctg ccagcatggt tttattggtt ggcggcgctc
     3661 ttttaccggt tggcaatagc ttgctgctca tttttggttt gttgtggata ttgttgatgg
     3721 tggttaccta tcagcgtctt cgccgcctac caacttggtc ttggcgacag gccggccagc
     3781 attccggcgc tatttggacg gccggagtta ttatcgtggt gattgcttta ttatctagct
     3841 taacttatct tagtaaaatt tatttagccg atattattgc cggtcgagcg gttaagaatt
     3901 cttctaccga tcagcagatt agtggagtgg ctgctgctca gcgtttagct ccatggcgtg
     3961 aaatgtatgg ttttagtttg gctcaattat cttgggttaa agccaatcag attgccgagg
     4021 ccgcggctgg tgctaccagt actgaggctc tagcggcgca aaatcaggct caagcctatg
     4081 ctcaacaagc taagcagttt ttggatcaaa atgctaaatt aattcaaaat caaccagccg
     4141 gtctcaagac gctagctgcg ctttatgaga tgattggcga ttttgatggt tctatggcta
     4201 cgcaacagcg tttaatcgct attgatccga ataatccttg gccgcaggct aaattggctc
     4261 aactcaaagt ggttatggct tatcaggctt caaccaaaga agataaagat aagctggttg
     4321 aagaggcgtt atctggttat cagcgagctt taactttaaa accaggctgg gctgaggctt
     4381 atttttatcg cgccaatctt tatcaggcta ttcaaaagtt ctccgaggct actgacgact
     4441 tagtgcaggc ggttaattat tctaacggtt cgccagatta tcagctcgcc ttagctcaat
     4501 tattaaatga gcgagctaaa gttgaaagcg ataagagcgc tgatttccga ttgcaagccg
     4561 agcaattggc taaaggtatt ttagccagtg attccaacaa cattaatgct ttatatgttt
     4621 tagcgataat ttatcgtgat agtggtcaaa ctgatttagc tcgtcagact gttcaagact
     4681 tgctgtctaa agctcaggac agtgacaaat caattattca gcaacaattt gccgatctct
     4741 tgccataggc tatgtcccca atgaccgcta accaattacg gcaaaaattt ttagattttt
     4801 ttgcttctaa acagcacgct attattccca gcgcttcact ggtgccggat aatgatccga
     4861 ccgttctttt taacacggcc ggcatgcaac cgttggtgcc gtatttatta ggagaagctc
     4921 atccgctcgg tcggcgcctg gctaatgccc agaaatgttt gcgcactgat gatattgatg
     4981 aagttggtga caccactcat ctgacttttt ttgaaatgct tggcaattgg tcgcttggcg
     5041 attatttcca acaggaatcc attacttgga gctgggagtt cttaacatca ccccaatggc
     5101 taaatctgcc gccagatcgg ttagcagcca ctgtttttat tggtgatcag catgcgcctt
     5161 ttgaccaaga agcttatcag ctgtggcaag aggttggctt gccagcggag cgtctagcca
     5221 aattaggcaa gaaagataat tggtggggtc cggccggtca gacaggacct tgcggcacgg
     5281 atacggagat tttttattgg accggctctg agccagcacc ggcagcttat aatccgtcag
     5341 ataagcgctg ggtagaaatt tggaataatg tcttcatgtc ttataataag caggctgatg
     5401 gcagttttca gccattagct cagcgtaatg ttgatactgg cttagggttg gaacgagtat
     5461 tagcgacgct gaataatcag cctgatattt atttgaatga tatttttcaa ccggctatag
     5521 cttatttaga acaagtcagc ggcttgtctt atactaacaa tcccagacca ctccggatta
     5581 ttgccgatca tctgcgtgcc agtatttttt tgttggctga tgaaaaagcg attactccga
     5641 gcaataaaga tcaaggttat gttttgcgtc gtctgattcg tcgggcggtg cgcttggccc
     5701 acaattggcc gcgttcaact gatgaagtct tagtcggcgt cgctgaatca attatccaat
     5761 caatgggttc agcttatccg gaattagtcg ctaagcatga ttttattgtt aatgaattaa
     5821 aaaaagaagt tactaaattt caagtgacat taaatcgcgg actcaaggag ctacagcgct
     5881 taattgaaac cggtcagccg attagcggtg ctgagacttt tatgctctat caaagttatg
     5941 gttttccttt agaaatgact aaagaaatcg ctagagagtc gggccgtcaa gttgatgaag
     6001 ttggttttca atcagagcta gttaagcatc aagaattatc acgcactgcc gctgccggta
     6061 agtttaaggg cggtttagct gatgattcgg ttgcaactac tcgtttacac acagctgccc
     6121 accttttatt gcaggctttg cgtcaagttc tcgggactga tgtttaccag cggggcagca
     6181 atattacgac tgagcgctta cgttttgatt tttcttatcc agacaaaatg accgctgagc
     6241 aaatcagtca agtggaggca atcgttaatc agcaaatcgc gctagaatta ccggttgctt
     6301 gccaagaagt ttcgttagat gaagctcgtt gtcaaggagc gatgggtatc tttgacgcta
     6361 aatacgggga aagggttaag gtttatacca ttggctctga gtcgccattc agtcaagaaa
     6421 tctgtggtgg ccctcatgtc cataatactg ccgaactcgg ccattttcgg attattaaag
     6481 aagaaagtag cagtgctggt gttcggcgga ttaaagctat tttagaataa tttatggata
     6541 agctaactat tttaataacc ggcggggctg gttatatcgg ttcacatgcc gttaaattat
     6601 ttttaagccg cggctatcgg gtggtagttt ttgataatct gtctcgcggt tggcggcagc
     6661 cgctagatat tttagcgccg ctaggcgatt tggtctttgt tcgtggcgac ttaactaaaa
     6721 ctgccgattt ggaccaactt ttcgctaatt ataattttgc cggc
//
LOCUS       DAOPAI010000028         6738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_10082_length_6738_cov_4.977256, whole genome shotgun sequence.
ACCESSION   DAOPAI010000028 DAOPAI010000000
VERSION     DAOPAI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10082_length_6738_cov_4.977256"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..140
                     /locus_tag="PKV49_03130"
     tRNA            64..140
                     /locus_tag="PKV49_03130"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Met,seq:cat)
     gene            157..229
                     /locus_tag="PKV49_03135"
     tRNA            157..229
                     /locus_tag="PKV49_03135"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:190..192,aa:Lys,seq:ctt)
     gene            301..999
                     /gene="truB"
                     /locus_tag="PKV49_03140"
     CDS             301..999
                     /gene="truB"
                     /locus_tag="PKV49_03140"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPL22633.1"
                     /translation="MTDYDSTINTSGFLLLDKPSGPTSHDMVYRLRRITGIKKIGHAG
                     TLDPLASGLLIMAISRAATKQIASLAKQDKVYEAVFQLGGTSDTEDRLGQIQPRTIKQ
                     IPDIKEIQLALNSFIGQTAQIPPMHSAKKIGGKKLYELARKGQIIERQPNLITIYKID
                     LLDYAWPQLKLTIHCSAGTYIRSIARDLGEKLGCGAYLAELRRTTIGNYSVTKAVSPD
                     KLTAENWTEYLLNN"
     gene            complement(1003..2802)
                     /gene="glmS"
                     /locus_tag="PKV49_03145"
     CDS             complement(1003..2802)
                     /gene="glmS"
                     /locus_tag="PKV49_03145"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPL22634.1"
                     /translation="MCGIIGYLGHQLAKDILLDGLKKLEYRGYDSAGLCILNDGQFFI
                     DKKVGPVVQLEQSVANQEIISTTGIAHTRWATHGAPSENNAHPHFDCHGQIAVVHNGI
                     IENYQALKEVLISEGHQFQSETDSEVLAHLIEKFYTDNLETAVTKALALVEGSYGLVV
                     LSIKENKLVAVRQSSPLIIGVGVGETFVASDIPAILNYTKQVIYLNDNDLAIIDSQGY
                     HLQNLAGQVVDRQVEEVKLNVEQIEKKGFKHFMLKEIFEQPQTISNVLAGRVKDGQIK
                     LSIDLDLSRLKRIIIAACGTSWHSALIGKYLLENIARIPVEVDYASEFRYRQPLVSDT
                     DLFVAISQSGETADTLAALREAKRQGAQTIAIVNVVGSTVTREVDSGIYLHAGPEIGV
                     ASTKAFTAQVTALILLALYLRQLVGQPLDDGLLADLNQLPEIVATTLSLNNQLADLAA
                     QFYHTDHFLYLGRGLNFPVALEGALKLKEISYIHAEGYPAAEIKHGPIALVDENMPVV
                     FLATKNDLSDKIISNIQEIKARGGRIIAVVDQDYDHIAELADHLIRVPVVNVLLSPIV
                     NAIPLQLLAYHIADYKGLDVDKPRNLAKSVTVE"
     gene            complement(2928..4919)
                     /locus_tag="PKV49_03150"
     CDS             complement(2928..4919)
                     /locus_tag="PKV49_03150"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPL22635.1"
                     /translation="MEITSMLIIGSALLAILYGAIAIRSILKLPRGNERMQEIAQAIQ
                     AGAKAFLNRQYRTVFMVAIPLFIIIGLVPHLGWTTAAAFLVGAVLSALAGYIGMNISV
                     RANVTTTEAAKSGIKKALAVAVKGGSITGMLVVGLALLGTAGFYFLTKDIHALIGFGF
                     GGSLISIFARLGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCAG
                     MAADLFETYTVTSIAAMILGALIFGHDNQTYIIFPLVLGAISILASVVGIGFIRLGKS
                     QKIMPALYKGLIASGLIAAIAFYPVISWLMKDNGQFSALNLYLASLIGLVVTAALVSI
                     TEYYTATKHAPVRQIASASTTGHGTNVIAGLAVSMKSTALPVVVIALAILGAYSLAGL
                     YGIAIAAVAMLSMAGIIVTIDAYGPITDNAGGIAEMAELGEDVRNVTDPLDAVGNTTK
                     AVTKGYAIGSAALASLVLFADFAAKAHGIKFHIDNPYVLIGLFIGGLLPYYFAALSMQ
                     SVGKAAGAVVKDVRAQFKAQPGIMDNTEKPDYGRTVDIVTKAAIKEMIVPSLIPVLAP
                     ILVGFIFGVQALGGLLMGSIITGIFVAISMTSGGGAWDNAKKYIEEGHFGGKGSDAHK
                     AAVTGDTVGDPYKDTAGPAINPMIKIVNIVALLIIGLLV"
     gene            complement(5039..5971)
                     /locus_tag="PKV49_03155"
     CDS             complement(5039..5971)
                     /locus_tag="PKV49_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPL22636.1"
                     /translation="MTTHQKLEIIQKTLGLTQTKLAEKFGVSFAAFNNWWTGKSCPRP
                     KMQSAIDELFLEITGQKIIPANQLIAKKQIIEQKLQNYQSVVSEILHNSDIRDQFILQ
                     LTYHSNSIEGSTLTEPDTAAILFDNTALPNKSLTEQLEAKNHQTALNYLFDYITKRGK
                     INERLILKFHSILMNGVHPDAGFYRRHGVRIVGVNLPTANYMSIPKLMSSLMADINKP
                     TQDIIALATNIHSHFEQIHPFSDGNGRVGRLLLNAMLLKANFAPAIIRQEQKQLYYTY
                     LYKAQTKNDHSQLEDFLCTAILDGFKILERSDIR"
     gene            complement(6128..>6738)
                     /locus_tag="PKV49_03160"
     CDS             complement(6128..>6738)
                     /locus_tag="PKV49_03160"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017032857.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HPL22637.1"
                     /translation="TEFHPTQILADFLTMTEHCDKPLHKISFCYLGDARNNMGNSLMV
                     GAAKMGMDFRAAAPKACQPSKELVATCKQIAKETGAKITITDNVAEAVKGVDFLYTDV
                     WVSMGEPAEVWTERIKLLKPYQVNMNVVKKTGNPNVKFLHCLPAFHNRETTIGEEIFQ
                     KYGLEAMEVTEDVFESPMSVVFDEAENRLHTIKAVMVATLGE"
BASE COUNT         2212 a         1470 c         1242 g         1814 t
ORIGIN      
        1 atcacgcttg gcttcggacc aagtattttg ggttcgaatc ctgacgggag cgctgatggc
       61 ccgggctggg tagctcagtt ggttagagcg tggcactcat aacgccaagg tcgtgggttc
      121 aattcccacc cccgccacca gaaaaataat gattatgcgc ccatagctca gttggtagag
      181 cagttgcctc ttaagcaaac ggtcgcaggt tcgagccctg ctgggcgtac taaaaagctt
      241 cccttgccgg ggaagttgtt tttttttggc taatctgtga ctgactgata taatcactat
      301 atgactgact acgattcaac aattaacacc agcggcttct tgctgctgga taaacccagc
      361 ggtccaactt cccatgacat ggtttaccga ttacgccgaa ttacgggcat taagaaaatc
      421 ggccacgccg gcaccttaga cccactagct tccggattgt taattatggc cattagccga
      481 gccgccacta agcaaattgc tagtctagct aaacaagata aggtttatga ggctgtcttt
      541 cagttgggtg gaaccagcga tactgaagac aggctaggtc aaattcaacc gcgaactatt
      601 aagcaaatac cggacattaa agaaatccag ctggccctaa atagctttat cggccaaact
      661 gcacagattc ccccgatgca tagcgctaaa aaaatcggcg gcaaaaaact ttatgaatta
      721 gccagaaagg gccagattat tgaaagacag cccaatctga taactatcta caagattgac
      781 ttgctggatt acgcttggcc gcaactaaaa ttaactattc actgctcggc tggcacatat
      841 atccgctcta tcgctcgcga tttaggtgaa aagcttggtt gcggcgctta tctggccgaa
      901 ttgagacgga ctactatcgg caattattca gtcaccaaag ccgtcagtcc ggataagctg
      961 acggctgaaa attggacaga atatttacta aacaattaga atctactcca ctgttaccga
     1021 cttggctaaa tttctaggtt tatccacatc caaacctttg taatcagcaa tgtgataagc
     1081 taatagctgt agcggaatgg cattaacaat tggcgataat aaaacattaa ccactggtac
     1141 tctaattaaa tggtcggcta attcagcaat atggtcataa tcttgatcaa ctacggcaat
     1201 aatccgcccg ccgcgcgctt tgatttcctg aatattacta ataattttat cgcttaaatc
     1261 attcttggtg gccagaaaaa ctaccggcat attttcatca actaaggcaa tcggaccatg
     1321 cttaatctca gcagccggat agccttcagc atggatataa gatatttcct ttagtttgag
     1381 cgcgccttct aaggctactg gaaaattaag tccccgtccc aaataaagaa aatggtcggt
     1441 gtgataaaac tgagcggcta aatcggccag ctgattattt aaactgagcg ttgtcgccac
     1501 aatctctggc aattgattca gatcagctaa taaaccatcg tctaagggtt ggccgactag
     1561 ctgccgcaaa tataaagcta gaagaattaa agcagtaacc tgggccgtaa aagccttagt
     1621 gctagccaca ccaatctcgg gtccagcgtg caaataaata ccggaatcaa cttctctggt
     1681 tactgttgag ccgacaacat taacaatagc aatggtttga gcgccttgtc gcttagcttc
     1741 acgcagggct gctaaggtat cggcggtctc accggattga gaaatggcga caaataaatc
     1801 ggtatcacta accaatggct gacgatagcg gaattcagac gcataatcaa cttcgacggg
     1861 aattctagca atattttcta ataaatactt accgatcaag gctgagtgcc agctagtacc
     1921 acaggcagca ataataatcc gcttcaatcg gcttaaatct aaatcaatag acaacttaat
     1981 ttgcccatcc ttgactcggc ccgccaagac attgcttatg gtttgcggct gttcaaaaat
     2041 ctctttcaac ataaaatgtt taaaaccttt cttttcaatc tgctcaacat taagcttgac
     2101 ctcttctact tgacgatcaa cgacttgacc ggctaaattc tgtaaatgat aaccctgact
     2161 atcaataata gctaaatcat tatcattaag ataaataacc tgtttagtat aattcaaaat
     2221 agctgggata tcggaagcga caaaggtttc accaacaccg acaccaataa ttaggggcga
     2281 gctttgccgc accgccacca acttattttc cttaatactt aagacaacta aaccatagga
     2341 gccttcaact aaagccagcg ccttagtgac ggctgtttcc aggttatcag tataaaattt
     2401 ctcaatcaga tgagccaaaa cttccgaatc agtttctgat tgaaattgat gcccttcgct
     2461 gatcaaaact tccttcaagg cttgatagtt ctcaataata ccattatgaa caacggcgat
     2521 ttgaccatgg caatcaaaat gaggatgagc attattttca ctgggagcgc catgagtagc
     2581 ccacctggtg tgagcaatac cggtggtgct aattatctct tgattggcta ccgactgctc
     2641 taactgaacc actggaccaa ctttcttatc aataaaaaac tggccatcgt tcaaaataca
     2701 aaggccagcg ctgtcataac cgcgatattc aagttttttc agcccatcca acaaaatatc
     2761 cttagctagc tggtggccaa gataaccgat aattccacac ataatgttaa ataaatattt
     2821 aaaatctgag cttaattatt ataataacaa ataattcaat ggagcgaaat acagaaaaac
     2881 accaagcctg acagcctggt gttttgctgt atgagtaata aaagctatta gaccaataaa
     2941 ccaattatca acaaggcgac gatattaaca atcttaatca ttgggttaat tgccggaccg
     3001 gcggtgtcct tatacggatc gccaacggta tcaccggtca ccgctgcttt atgagcatca
     3061 gaacctttac cgccaaaatg accttcttca atatattttt tggcattatc ccaggcgcca
     3121 ccaccggagg tcatagaaat ggctacaaaa ataccagtaa taatagaacc cattaataag
     3181 ccaccgagag cttgaacacc aaaaataaaa ccaactaaaa taggcgctaa aacaggaatt
     3241 agtgatggga caatcatttc cttaatagct gctttagtta caatgtcgac agtgcgacca
     3301 taatctggct tttcggtatt atccataata cctggctgtg ccttaaattg tgcccgcaca
     3361 tctttaacta cagcaccagc ggctttacca acagattgca ttgatagggc cgcaaaataa
     3421 taaggcaata aaccaccaat aaataaacca attaaaacgt agggattatc aatgtggaat
     3481 ttaatgccat gagctttagc ggcaaaatca gcaaataaaa ctaaggaagc taaagcagct
     3541 gaaccaatag catagccctt ggtaaccgct ttagtcgtat tgccaaccgc atctaatgga
     3601 tcggtcacat tacgcacatc ctcaccgagc tcagccattt cggcaatacc gccggcgttg
     3661 tcggtaattg gaccataagc atcaatggtg acaataatgc cagccataga tagcatcgca
     3721 accgcagcaa tggcaatacc atataaacca gccagagaat aggcacctaa aatagctaaa
     3781 gcaataacaa caactggtaa agcagttgat ttcattgaca cagctaagcc agcaattaca
     3841 ttagtaccat gaccagtcgt ggaagctgag gcaatttgtc tgactggagc atgtttagtt
     3901 gcagtatagt attcagtaat gctaaccaaa gcggccgtaa cgactaggcc aatcaatgaa
     3961 gctaaataca aattcagagc actaaattga ccattatctt tcattaacca actaataact
     4021 ggataaaaag caatcgctgc aataaggcca gaagcaatta agcctttata tagagctggc
     4081 ataattttct gtgatttacc caaacgaata aaaccaatac caactactga agcaagaatc
     4141 gagatagccc ccaggactaa gggaaaaata atatatgttt ggttatcatg gccaaaaatc
     4201 aaagcaccca aaatcatagc agcaattgag gtaaccgtat aagtttcaaa taaatcagca
     4261 gccataccag cacaatcgcc aacattatca ccaacattat cagcaataac cgctggatta
     4321 cgcggatcat cttcgggaat accggcctca actttaccaa ccaaatcagc gccaacatca
     4381 gccgccttgg taaagatacc gccgcctaaa cgagcaaaaa tagaaatcaa cgaaccacca
     4441 aaaccaaaac cgattaaagc gtgaatatct ttagttaaaa aataaaaacc agctgtaccc
     4501 agtaaagcta gaccaactac caacatgcca gtaatactgc cacctttaac agccacagct
     4561 aacgcttttt ttattccgga cttcgccgct tcagtggtgg taacattggc tcggaccgaa
     4621 atattcatac caatataacc ggccaaggct gataacacag caccaactaa aaaagctgcg
     4681 gccgttgtcc agcccaaatg aggcactaag ccaataataa taaacaacgg aatcgccacc
     4741 ataaaaaccg tccgatactg acgatttaaa aaggccttag cgccggcttg aatagcttga
     4801 gcaatttctt gcatgcgctc attaccgcgg ggcaatttca aaattgacct gatagcaata
     4861 gcgccataga gaatagctag taaggcgcta ccaataatca gcattgaagt aatttccata
     4921 acccaatggt tagaatgaat aaaaaagata aaatcagcac caaaataggg ctgttataac
     4981 taatatctta gcaaaatata gggaaaaagc aagttaaaga atggtgatta gaaaattatt
     5041 agcgaatgtc tgatcgctcc agaatcttaa atccatccag aatagcagtg cacaaaaaat
     5101 cctctaattg gctgtggtca tttttggttt gagctttata aagataagta taatatagtt
     5161 gtttttgttc ttggcgaata atagcggggg caaaattagc ctttaacaac atagcattaa
     5221 gcaataatcg accaacgcga ccattgccat cactaaacgg atgaatctgt tcaaaatgac
     5281 tatgtatatt agtagctaaa gcaataatat cttgagtcgg tttatttatg tcagccatca
     5341 gagaagacat taatttagga atgctcatat aattagctgt cggtaaatta acaccaacaa
     5401 tcctaacacc gtggcgacga taaaaaccag catcaggatg aacaccattc attaaaatgc
     5461 tgtgaaattt gagtattaat ctttcattaa tctttcctcg tttagtaata taatcaaata
     5521 aataatttaa ggctgtctga tgatttttgg cctctaattg ctcagttaag ctcttgttgg
     5581 gtaaggcagt attgtcaaaa agaatagcgg ctgtatcagg ttcagtaagc gtgctaccct
     5641 cgatactgtt actatgataa gttagttgta aaataaattg atcgcgaata tcagagttat
     5701 gcaaaatttc actaacaaca ctttgataat tttgcaattt ttgctcaata atttgttttt
     5761 tagcaattaa ttgattagct ggaattattt tttgtccagt aatctctaaa aataattcat
     5821 caatagcgct ttgcattttg ggtcgaggac aagacttgcc agtccaccaa ttattaaagg
     5881 ccgcaaaaga aacaccaaat ttttcagcta atttagtttg agttaatcca agtgtttttt
     5941 gaattatttc cagcttttga tgagtggtca taaaatcatg ttacaataga tatgatcatt
     6001 ttaacaaact ttataaagtt gggcaaaata cataaacttt ataaagttta tgattgataa
     6061 aaagtaaaat aaaaaatctg ctgttacgag taatcataac agcagatttt atctaaacac
     6121 agatagttta ttcgcctaat gtcgctacca tcactgcctt aatggtatgt aaacggtttt
     6181 cggcttcatc aaagacaact gacatgggtg attcaaaaac atcctcagtc acttccatcg
     6241 cttctaaacc atatttttga aaaatttctt ctccaattgt tgtttcccga ttatgaaatg
     6301 ccggcaagca atgtaaaaac ttaacattgg gattgcctgt ttttttaacg acattcatat
     6361 ttacttgata gggtttcaat aatttaattc tttctgtcca cacttcggcc ggttccccca
     6421 ttgacaccca cacatctgta tataaaaaat cgacaccctt aacggcctca gccacattat
     6481 cagtaatagt tattttagca ccggtttctt tagctatttg cttacaagta gctaccaatt
     6541 ctttacttgg ttgacaggct ttgggagcgg cagctctaaa atccattccc attttagcag
     6601 caccaaccat taatgaatta cccatgttgt tacgggcatc tcccaaataa cagaaagaaa
     6661 ttttatgcag aggtttatcg caatgttctg tcattgtcaa aaaatcagct agtatttgag
     6721 tgggatgaaa ttcggtgg
//
LOCUS       DAOPAI010000029         6037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_11491_length_6037_cov_3.541625, whole genome shotgun sequence.
ACCESSION   DAOPAI010000029 DAOPAI010000000
VERSION     DAOPAI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11491_length_6037_cov_3.541625"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKV49_03165"
     CDS             <1..358
                     /locus_tag="PKV49_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPL22638.1"
                     /translation="ELKKKRKIYFYDLGLRNALINNLNPISLRQDIGALWENFMVMER
                     VKRNNNLGITANIYFWRTTDGKEIDYVEDSGGTLAGYEFKWQKSNFSIPKEFLQAYDG
                     SVVKLVNRDNYLELLL"
     gene            386..868
                     /locus_tag="PKV49_03170"
     CDS             386..868
                     /locus_tag="PKV49_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22639.1"
                     /translation="MEGDQALVFEQFKKASENKYRDIKSLFCPALGRAVVFNADGFHH
                     LRYYNSRSERPKRVQVNKLTCFKNVPDVLSKTTTIQEYRKTICPTGKADRSGYRAVSV
                     VEWFGFIAIINLEKKIRIKIVVRRIGQQDGKYHFWSVMPYWRLNSGIRITGAADLEDD
                     "
     gene            1140..3341
                     /locus_tag="PKV49_03175"
     CDS             1140..3341
                     /locus_tag="PKV49_03175"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPL22640.1"
                     /translation="MSRPWYRFVLKLGVYASLVSLFLVNKGYYFPYITSKQLYFNILL
                     EVMAVIWLAFIWKYPADRPKKSWLTIGWLGYLAVVLLTCLTGVDFNLSFWGDLERMLG
                     WFPLFHFFLLYLIVITVFRTWRDWQPLLMTMVGAGTLLSLVGIFQNYSASTLGNAAYM
                     GTLMLMSMFIAALLIGRSRNWLTAAPYWLVLVLLFVGLIRADISGAYAGLAAGALLYA
                     GLMTFLSEHKTARRIGWVSLGLLVVGIGAVFALRWSPALDNTQLGKVFRDFSLQNNTL
                     NTRLYSWTWAWRDLPRHPILGVGYGNFAFVFDRQFQGEFYKFSKGEDYFDRAHNNIVE
                     IATTTGLLGLAAYLFMFSWAVIYLIRAYRHKKIDLPEFAALAGFLMAYFVQNLALFDC
                     LASYLFLMIILGFIHNLDKLHEPTVRPSDSDWTSREVAILVVGGLLAAWLAWAANITT
                     MRMLTKTIDGIIKVNQGQAMDALAAFKQAQAERSPLIRDARSAMVNAFVSGSYVLEKL
                     PITQLGEILSYGSQATIDNLKYNPNDSFLNMQRAQFLSLASQISRNNLSVSYSRDGLA
                     AVEKSIANGPEHILPYYVKAQLLSDLNQPDEAIATLKQALAISPDYWDTYCHLSRLQL
                     QAQVDMNEAYGYLDQCLDKGGVTMLGPGNYLNEAIKHYQQADDVERLTLLTEQLVRIY
                     PQGAEIWLKLADLYHKQNRPEEAQYAAQQAIQLKPEYQKQLEGILRAAESK"
     gene            3423..3584
                     /locus_tag="PKV49_03180"
     CDS             3423..3584
                     /locus_tag="PKV49_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22641.1"
                     /translation="MKKLRQILKSWKQKIIQDLSLDEAFRYLAVINRASQQKIKWGSI
                     LEIVIGVYL"
     gene            3603..5078
                     /locus_tag="PKV49_03185"
     CDS             3603..5078
                     /locus_tag="PKV49_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPL22642.1"
                     /translation="MEKIKDNEPIKVLIINPPRFNGQNVRRDERSADILPGEVSPFYQ
                     GAVLAQYLREKHGVVVDVLDANGLDLDFPAVEQWIKTRSSYKHVIIKAADDTLLHDIV
                     VGQIAKQYGLTTFLWEPILSPAAPERVLYEINRGKQSIDYLILCEAEVTFSDFLLYGP
                     EAHGLAFIKDNKFIINQRLENERLADLNSLPIPSFQDLPVDKYQAWFGEKPWMTLFTS
                     RGCIGTCSYCLIGGSTISRGYGRCIRLQSPERIFQETSLLISKFGVKHITFWDDCFTL
                     NRDRVVRFCNLVIENGLHFNWSCMSRADLIDEDLVILMKKAGLTKIGFGVESGSQKIL
                     DSIPKKVTVQQNMEAITMCHRHGLWVWIFLIIGLPEENWSTVKETINFVKRSKPDYLF
                     SGCATPFPGTVYYNQCQQYGLFDGDIFDVIAREAVATGAQPKAKSKYLSEVELKKAEF
                     LIHRAFLFSSFKVIISKIRENKNKLSVAYLWSKFKFFFLAN"
     gene            5145..>6037
                     /locus_tag="PKV49_03190"
     CDS             5145..>6037
                     /locus_tag="PKV49_03190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPL22643.1"
                     /translation="MFNLVKEGSKYFSVHFLFVNHGQQKIDLGDCRLALPGVVFHQVV
                     ADNSKFKQLFYLFLRIFGCQFFCYREIKRTINNIVLDHRIDVVQFEFSQMIRYCDKRL
                     AAKKALVVHEIIFRSLWRQLSVSSSFKEKVLNFGRFLFFKLEEINALKDFDLIIAMSN
                     EDRLYLNQFTRRRDIEIIPNGVNTAGFSFSKDICPSRQLYFIGWFKNLQNVDAMDFFL
                     NELLPVCSNRGVDFNMKIVGKDASDDLKNSFTKHGFDYIDYLPENMFSSLANSVLIVP
                     LRFGGGTRLKILEAMSMGNPV"
BASE COUNT         1625 a         1079 c         1344 g         1989 t
ORIGIN      
        1 tgaattgaag aaaaaacgaa aaatttattt ttatgacttg ggtttgcgca atgctttaat
       61 aaataacctt aatcctattt ctttacgcca agatattggc gctctctggg aaaattttat
      121 ggttatggaa cgagtgaaga gaaacaataa tcttggtatt acagctaata tctatttttg
      181 gcggacaacc gacggcaaag agattgatta tgttgaagat tcaggtggaa cacttgcggg
      241 ctacgagttc aaatggcaaa agagtaattt ttccataccc aaggagtttt tgcaggccta
      301 tgacggcagc gtcgtcaaat tggttaatcg tgataattat ctggaattat tactttgatt
      361 ggtattatgt actaatttaa tttttatgga gggggatcaa gcgttagttt ttgagcaatt
      421 taaaaaggcg tcagaaaata aataccgaga cattaaaagc cttttttgcc cagccctggg
      481 gagagcggtg gtttttaatg ctgacggttt tcatcattta cgttattata acagtcggag
      541 tgaacgacca aaacgagtcc aagttaataa attgacttgc ttcaaaaatg ttcctgatgt
      601 tctatctaaa accaccacaa ttcaagaata caggaaaacg atttgtccaa ccggcaaggc
      661 agatcgcagt ggctatcgtg cggtaagcgt ggtggagtgg ttcggcttta tagctattat
      721 taacttagag aaaaaaatta gaattaagat agtggttagg cgaattggtc aacaagacgg
      781 taaatatcac ttttggagtg tcatgcctta ttggcggtta aatagcggca tcaggattac
      841 tggtgcggct gacttagaag atgattaaaa aacaaaaaca ccctcagatg agagtatttt
      901 tgttgtgctt gcttagcggc ttttagttca ttgctgaact tagccgaacg gggccttcgc
      961 ccacaagcac tatttcatga taggtgattt tatgattttt gtaaagatta gcctatcggc
     1021 ttaatttgat ttactcaaaa aatcggccaa ttaatctttt tcttgccttc ccgttagttt
     1081 ttctctatac ttaaagcagc gttttagcgc tgctttttgc ttaattatta ctcaatatta
     1141 tgtcgcgacc ttggtatcgc tttgttttga agctgggggt ttatgccagc ttggtttctt
     1201 tatttttggt caataaaggt tattattttc cttacatcac ttccaagcag ctttatttta
     1261 acattttatt ggaggttatg gccgttatct ggttggcctt tatttggaag tatccagccg
     1321 atcggcctaa gaagtcatgg ctaactattg gttggttggg ctatctggct gtggtgctct
     1381 tgacttgttt gactggtgtt gatttcaatc ttagcttttg gggcgatttg gagcgcatgc
     1441 tcggctggtt cccactcttc cattttttct tattatacct aattgttatt accgttttcc
     1501 gcacttggcg cgattggcag ccgctactaa tgaccatggt tggcgccggc actttgctta
     1561 gtttggttgg tatctttcaa aattatagtg cctctacttt gggcaatgcc gcctatatgg
     1621 gcacgctgat gctgatgtcc atgtttattg ccgctctgtt gatcggccgt agccgtaatt
     1681 ggctaaccgc cgcgccctat tggttggttc tagtgcttct ttttgttggc ctcatccggg
     1741 ccgatatctc cggcgcttat gctggcttag cggctggcgc cttgctttat gccggattga
     1801 tgactttttt aagtgagcac aagactgcta ggcggattgg ttgggttagt ttagggttat
     1861 tggtggtcgg catcggcgcc gtctttgctt tgcgttggtc gccagcacta gacaatactc
     1921 aattgggcaa ggtttttcgt gatttctctt tacaaaacaa tactcttaat acacggctct
     1981 actcctggac ctgggcttgg cgcgatttac ccagacatcc gatcttgggg gttggttatg
     2041 gtaactttgc ctttgttttt gaccgtcaat ttcagggcga attctataag ttctccaagg
     2101 gtgaagatta ttttgatcgg gctcacaata atattgtgga gattgccact accactgggc
     2161 tacttggtct agctgcctat ctgtttatgt ttagctgggc ggtgatttat ttaattaggg
     2221 cttatcgtca taagaaaatt gatttgccag aattcgccgc cttggctggc tttctgatgg
     2281 cttattttgt ccaaaatctg gccctctttg actgtttagc tagttatctg ttcctgatga
     2341 ttattctagg atttatccac aatttggaca aacttcatga gccaacggtt cgtcctagtg
     2401 atagcgattg gactagccgt gaagtagcca tcttggtagt tggcggtctg ttggcggctt
     2461 ggctagcttg ggcggccaat attaccacca tgcgcatgct gaccaagaca attgacggaa
     2521 ttattaaggt caatcagggg caggctatgg atgctttagc ggctttcaag caagctcagg
     2581 ccgaaaggtc gccgctgata cgcgatgctc gctcagccat ggttaatgcc tttgttagcg
     2641 gcagttatgt cttggaaaaa ttgccaatta cccagctcgg tgaaatcttg agctatggta
     2701 gtcaagccac aattgacaat ttgaaatata atcccaacga tagtttctta aatatgcagc
     2761 gggcccagtt tttgtcgtta gctagccaga ttagtcgcaa taatttatcg gttagctata
     2821 gtcgtgatgg tttagcagct gtggaaaaat ctattgctaa tggcccagaa catattttac
     2881 cctattatgt caaagcccag ctcctgtctg atcttaatca gcccgatgag gccatcgcca
     2941 ctctcaaaca ggccttagct atcagcccgg attactggga tacttattgc catcttagcc
     3001 gtttgcagct gcaagctcag gtggatatga acgaagctta tggctatttg gatcaatgcc
     3061 tagataaggg cggggtaacc atgctcggcc cgggcaatta tttgaacgag gccatcaagc
     3121 attaccagca agccgacgat gtggagcgct tgaccctgtt aaccgagcaa ttagttcgta
     3181 tttatcccca gggcgctgaa atctggctga aactggccga tctctatcac aagcagaacc
     3241 gtcccgaaga agctcaatac gccgcccagc aagccatcca gctcaagccg gaataccaga
     3301 agcagttaga ggggatactg agggcggctg aatctaagta acttcaggca ataaccttaa
     3361 tttttgtttc ataatttcag gctataacct tatgaagaat gggtaattcc gagtcaaatt
     3421 ttatgaaaaa acttaggcag atcttaaagt cttggaaaca aaagattatc caagatttat
     3481 cacttgatga ggcttttcgt tatttggctg ttattaatag ggctagccaa cagaaaatta
     3541 aatgggggag tattttggag attgttattg gcgtttattt atagttaaaa ttgactctga
     3601 atatggaaaa gattaaagat aatgaaccaa tcaaagtttt aatcattaat ccaccgagat
     3661 ttaatgggca aaacgttcgt cgagatgagc gtagcgccga tatattgcct ggtgaagtta
     3721 gcccatttta tcaaggagct gtcttagccc aatatttaag agaaaagcat ggagttgttg
     3781 ttgatgtttt agatgccaat ggtcttgatt tggattttcc ggccgtggaa caatggataa
     3841 aaacaaggtc tagttataaa catgttatta ttaaagctgc cgacgataca ttgcttcatg
     3901 atattgtggt tggacagata gccaagcaat atggcttgac cacttttctt tgggagccga
     3961 tattgtctcc agctgcccca gaaagagttc tctatgagat taataggggt aaacagtcta
     4021 ttgactacct tattttatgt gaagccgaag taacttttag cgatttttta ctatatggcc
     4081 cagaagctca cggattagct tttattaagg ataataaatt tattatcaat cagcgattag
     4141 aaaatgagag attagctgat ttaaatagtt taccaatacc aagtttccaa gacctacctg
     4201 ttgataagta tcaagcctgg tttggtgaga aaccatggat gaccttattt acctctcgtg
     4261 gctgtattgg cacttgtagc tattgtttga ttggtggtag tactattagt cgtggttatg
     4321 ggcgctgtat tagattgcaa agtccagaaa gaatttttca agaaacaagt ttgttgattt
     4381 ccaaatttgg agtaaaacat ataacttttt gggacgactg ctttaccctt aacagagata
     4441 gggttgtaag attttgcaac ctagttattg aaaatggttt gcattttaat tggagttgca
     4501 tgtcccgcgc cgatttaata gatgaagatc ttgttatttt aatgaaaaaa gccggcctaa
     4561 ctaagattgg ttttggagtt gagtccggta gccaaaagat tcttgattct atacccaaga
     4621 aggtcacagt ccagcaaaac atggaagcaa ttacaatgtg ccatcgtcat ggcttgtggg
     4681 tttggatatt tttgattatt ggtttgccgg aagaaaactg gtcaaccgtt aaagaaacca
     4741 ttaattttgt caaaagatca aagcctgatt atcttttttc tggttgtgcc acccccttcc
     4801 ctggaaccgt ttactataat caatgtcagc aatatggctt gtttgatggg gatatttttg
     4861 atgttattgc tagagaggct gtagctactg gagcacagcc aaaagcaaaa tcaaaatatt
     4921 tatcagaagt agagctaaag aaagctgaat ttttaattca tcgtgctttt ttatttagct
     4981 cgttcaaagt gataatctca aaaattaggg aaaataaaaa taagttgagc gttgcttatt
     5041 tgtggtcaaa atttaagttt ttctttttgg ccaattagta tatgaagatg cttgttgtta
     5101 gtccattctt gccctaccct gttgattctg gcggcaagac caggttgttc aatcttgtta
     5161 aagagggtag taaatatttt tcagtgcatt tcctgtttgt taaccatggc cagcaaaaaa
     5221 ttgatttagg agattgtcgg ttagccttgc ctggagttgt cttccatcaa gttgttgccg
     5281 ataattcaaa atttaaacag cttttttatt tatttttgcg tatttttggt tgtcaatttt
     5341 tttgctatag agaaattaaa agaaccataa acaatatagt acttgatcat agaattgatg
     5401 ttgtgcagtt tgaattctcg caaatgataa gatattgcga caaacgcctt gctgctaaga
     5461 aggctttagt ggtccatgag attattttta gaagtttgtg gcgtcaacta tcagttagtt
     5521 ctagttttaa agaaaaagta ttaaactttg gacgttttct tttttttaag cttgaggaaa
     5581 tcaacgcgct taaagacttt gacctaatta ttgccatgag caatgaagac cgcctttatt
     5641 taaatcaatt taccagaagg agagatatcg agataatacc caatggagta aacacggctg
     5701 gtttttcttt ttctaaggat atttgcccca gcagacagct ttattttatt ggttggttta
     5761 aaaatttgca gaatgttgat gctatggatt ttttcttaaa tgaattatta ccagtctgct
     5821 ctaatcgggg cgtggatttc aatatgaaaa ttgtaggcaa agacgcgagt gatgatctaa
     5881 agaatagttt tactaaacat ggttttgatt acattgatta tcttcccgaa aatatgtttt
     5941 ccagccttgc caatagtgtg ctaattgttc ccctgcgttt tggcggcggc actagattaa
     6001 aaatattgga ggcaatgtct atgggtaatc ctgtgtt
//
LOCUS       DAOPAI010000030         5899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_11770_length_5899_cov_3.488193, whole genome shotgun sequence.
ACCESSION   DAOPAI010000030 DAOPAI010000000
VERSION     DAOPAI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11770_length_5899_cov_3.488193"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKV49_03195"
     CDS             <1..238
                     /locus_tag="PKV49_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPL22644.1"
                     /translation="ARALVYQPKILLADEPTGNLDSINAEEIIELLQKINKFGTTVIL
                     VTHNREIVNNLNKRVLTISDGRLVSDQARGKYII"
     gene            243..1154
                     /locus_tag="PKV49_03200"
     CDS             243..1154
                     /locus_tag="PKV49_03200"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPL22645.1"
                     /translation="MFLTLSRIVKFGFQDVWRNFWLSLATIMILILTLFSVNLLLSLR
                     IIGQAAVDNLKNRVDISIYLKEGVSEDKALGLKARLANLDNVQEVSYVSQADALEAFR
                     RQHQDNPEILQALQELDKNPLTSSLTIKPRDPDQYNQLIEQLSQIQDPLIGSRNFQDN
                     QALLAKINNITNKINDIAIVISSIFVLIMLLVIFNSVKLAIHSHRQEIAIMKLVGASN
                     FFIKAPFLFSSIIYTVAGVILTAAIFYLFLNVLQPYLDTFFFGYNIDLLQYFNHYWWQ
                     IAGLQLLGIGTVNLLASYIAVGKYSRI"
     gene            1157..1843
                     /locus_tag="PKV49_03205"
     CDS             1157..1843
                     /locus_tag="PKV49_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPL22646.1"
                     /translation="MLSQSQEKLIKSLHTNKGRAKAGLWLIEGKKFLELAAPNIQLTF
                     TAADTANFRQLTTTDSLPTIAALAQPPKFQLADIKRQKTILLLDNIQDPGNLGTIIRL
                     AGAFKAGLILLNCVDPGNPKVIRSSAGTIFKTPWLNLDWPAAKQLIQELNRPIYRLEN
                     NHQAQALNIKNNNLPSDILLIVGSEGQGITVPLSGKTLFIDHQPWVESINVATAVAIA
                     LFVRYQTPNI"
     gene            1840..2889
                     /locus_tag="PKV49_03210"
     CDS             1840..2889
                     /locus_tag="PKV49_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HPL22647.1"
                     /translation="MNKNFEHSELRAIQYGCGKMGKLILRYLYEKGVTIVGAIDSNPS
                     LIGQDIADLIGLEQKLGITISGNPEEVFAKQPADICIVAIGSLMTEMYPLLTIPLKYG
                     VNTITTCEEAFFPWTTSPEFSKRLDSLAKDNYCTLTASGYQDVFWGNLITVLAGASHK
                     IIAIEGQASYNIEDYGIALAKIHGVGLPSAEFAAKISQANILPTYMWNSNEWLAARLG
                     LTVKNINQKITPVIAEQPTASRLLNRTIAAGECLGMTATIDLETNEGPIIQAKSTGKI
                     YGPADQDTNNWTIKGQPDINVTITQPATVELTCATIVNRIPEIMVANPGFLTTHNWPS
                     PSYRGRILPSYLPTK"
     gene            complement(2916..2986)
                     /locus_tag="PKV49_03215"
     tRNA            complement(2916..2986)
                     /locus_tag="PKV49_03215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2952..2954),aa:Gln,seq:ttg)
     gene            3053..4135
                     /locus_tag="PKV49_03220"
     CDS             3053..4135
                     /locus_tag="PKV49_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPL22648.1"
                     /translation="MLKSCYRLIIINLLIMASWPGQVMAATQSNPALAEQQATITAIK
                     KALPAIVSITVSETTKQKIDGQLVNQKIRRGSGTGFIISSDGLILTNRHVVNQAGDKG
                     EYRIRFQSGKSYYAQLIDKDPLNDLAILKIFDKNLPTIQLGNSQDLVMGTTVIAIGNA
                     LGRYQNSATKGIVSGIGRNLSASNDNGQDELLTNVIQTDAQINPGNSGGPLIDLNGRV
                     VGINVARDASGGSIGFAIPIDDAKPVIDSVKKLGRIVRPRLGVSYLTINDDLALEKDL
                     PVNQGALISANNDQPAVIAGSPAERAGILPGDIILEINAQKLNEQNSLLQIIQKYQPN
                     QKIGLKIQRGDKKIIVQATLDEFPNR"
     gene            4140..5126
                     /locus_tag="PKV49_03225"
     CDS             4140..5126
                     /locus_tag="PKV49_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPL22649.1"
                     /translation="MKKKFDIITIGGATEDLSFLINDYRLLDNRREILAKKLLAIEYG
                     AKTIVDESFVEYGGGAANLAMAIKQCGLSVASWLAVGSDRRGQDIINNLKSHKIDTGL
                     CQIIDRQQSAVSVVLIGQDGEHTIIAYRGANDHLSITSSAAKQLASDWLYVSSLSGKI
                     WLKNLTAIFKSHKKIAWNPGQAQLTAGFKKLRPFLEKTDLLIINQDEALQLLASHPFD
                     NKQLADYGQNMKKLIQRLWCYGPKIVVITADSRGAWSYDGQTTIHQPALPVKKIIDTT
                     GVGDAFGGTLLGCLIKTNSLKKSLYLAAKQAAAAISQRGAQSGLINISKLIR"
     gene            5130..>5899
                     /gene="mutM"
                     /locus_tag="PKV49_03230"
     CDS             5130..>5899
                     /gene="mutM"
                     /locus_tag="PKV49_03230"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPL22650.1"
                     /translation="MPELPEVQTIVNDLQPLIMNKYIIRLQILDSRLKNLNTKPSLQL
                     IIGQPITNIFRRAKNLVIELANNYYLVVHLKMTGQLIWTSNEQWLAGGHPTTDLLSSN
                     QASWPNKSTRLIIELSNHDRLFFNDSRKFGWVKLMNKQEWLQYQTRLGIEPLSSNFSW
                     TDLQLILKKKPTTKIKQVLLDQHCLAGIGNIYADESLFAAGIKPSRPAGTLTMIEIKK
                     LHSSIVKILTTAVAYRGTSFSDYRDGRGQSGNFSQQLQV"
BASE COUNT         1833 a         1188 c         1141 g         1737 t
ORIGIN      
        1 tgctcgagct ttagtttatc agcctaaaat cttattagct gatgaaccaa ctggtaattt
       61 agactcaatt aatgccgaag aaattattga gcttttacag aagattaata aatttggcac
      121 tactgttatt ttagttactc acaaccgtga aattgttaat aatcttaata aacgagtttt
      181 aacgattagc gatggccgac tagttagcga ccaagcgcgc ggcaaatata ttatttaaaa
      241 ttatgttctt aactttaagt agaattgtta aatttggctt ccaggatgtt tggcgtaatt
      301 tctggttgtc actagcgacc atcatgattt taattttgac gctattttca gttaatctcc
      361 tgttatcatt aagaattatt ggtcaagccg ctgtagacaa tctaaaaaat cgcgttgata
      421 tttctattta tctcaaagag ggggtatccg aagataaagc tctcggactt aaggcccgct
      481 tagctaattt agacaatgtg caagaagtta gctatgtatc tcaagctgat gccttagaag
      541 cgtttcgccg tcaacaccaa gataatccag aaattctaca agcattgcaa gaactagaca
      601 aaaatccatt aacttccagc ttaaccatta agcctcgtga tccggatcaa tataatcaat
      661 tgattgaaca attaagccaa attcaagatc cactgatcgg ttcgcgtaat tttcaagata
      721 accaggccct attagctaag attaacaata tcactaataa aattaatgat attgctatcg
      781 ttattagttc aatctttgtc ctaataatgc tcttagttat ctttaattcg gtcaagctag
      841 ctattcatag ccatcgtcaa gaaattgcta ttatgaaact ggttggtgct tctaattttt
      901 ttattaaagc gccgtttctc ttttccagta tcatttatac cgtggctggc gtaattctaa
      961 ccgcggccat tttttatcta tttttaaatg ttctccaacc ttatttagat acttttttct
     1021 ttggttataa tattgattta ttgcaatatt tcaaccatta ttggtggcaa attgccggtt
     1081 tgcaattgtt gggcatcggc actgttaatt tactggctag ttatatcgcc gtcggcaaat
     1141 attctcgcat ctagctatgc tcagccagag tcaagaaaaa ctgatcaaat cgctacacac
     1201 taacaaaggc cgcgctaagg ccggcttatg gctcattgaa ggtaaaaaat ttttggaact
     1261 agcagcgcca aacattcaac taacttttac tgccgctgac actgccaatt tccgtcagct
     1321 gactaccact gatagtctgc cgactattgc cgccctggct caaccgccca aatttcaact
     1381 agcagatatt aaacgccaaa aaactatctt gttattagac aatatccaag accctggtaa
     1441 tcttggcact attatccgtt tggccggcgc ttttaaggcc ggactgatcc tgttaaattg
     1501 cgttgatccc ggtaatccta aagttattcg ttcttccgcc ggcaccattt ttaagactcc
     1561 atggttaaat ctagattggc cggcggctaa gcaactaata caagagctga accgaccaat
     1621 ctatcgtcta gaaaacaacc accaagcgca agctttaaat attaaaaata ataatttacc
     1681 gtcagacatt ttgctgattg tcggctcaga aggtcagggg attaccgtgc cactatcggg
     1741 aaaaacattg tttattgatc atcaaccatg ggttgagtct atcaatgtgg cgaccgccgt
     1801 ggccattgct ttatttgttc gttatcaaac tccaaatata tgaataaaaa ttttgaacat
     1861 tcagaattac gcgctatcca atacggttgc ggtaaaatgg gtaagttaat attacgttat
     1921 ctttatgaaa agggggtaac gattgtgggc gcgattgata gtaatccgtc tttaattggt
     1981 caagacattg ccgatttaat cggcttagaa caaaaattag gtatcaccat cagtggcaac
     2041 cctgaagaag tttttgctaa acaaccagcc gatatctgta tcgtagccat tggcagctta
     2101 atgacagaaa tgtatccatt actgactatt ccactaaaat atggcgttaa tactattact
     2161 acttgtgagg aagccttttt cccgtggacc acttcaccgg aatttagcaa acgccttgat
     2221 tcattagcta aagacaatta ttgcacgcta acggctagtg gttaccaaga tgttttttgg
     2281 ggaaatttaa ttaccgtttt ggccggcgcc agccataaaa taatcgccat tgagggtcaa
     2341 gctagctata atattgaaga ctatggtatc gccctagcta agattcatgg agtcggtttg
     2401 ccgtcagctg agtttgccgc caaaatcagt caagccaata ttttgccgac atacatgtgg
     2461 aatagcaatg aatggctagc agccagatta gggttgacgg ttaaaaatat taatcaaaaa
     2521 ataaccccag ttattgctga gcaaccaact gccagtcgtt tgcttaatcg gacgattgct
     2581 gctggcgaat gtctgggcat gacagccact attgatttag aaactaatga aggaccgatt
     2641 attcaagcta aaagtactgg taaaatttat ggaccagctg atcaagatac taataattgg
     2701 acgattaaag gccaacccga tattaatgtc actattactc aaccagctac ggtcgaacta
     2761 acttgtgcca ctattgttaa tcgaattccc gaaattatgg tcgctaaccc cggttttctg
     2821 actacccata attggcccag ccctagttat cgcggacgaa tattgcctag ctatttgcca
     2881 acaaaataac atcgccctaa agcgatgtta taatgctcgg ggacagggat tcgaaccccg
     2941 attaatggct ccaaaggcca ctgtcctacc attagacgat ccccgaaaaa ctttatcaat
     3001 ataacgcact gctaaaattt tgtcaaaaat taaattatta tatattacaa caatgttaaa
     3061 gtcatgttat cggctaataa ttatcaattt attgataatg gccagctggc ctggtcaggt
     3121 tatggcggct actcagagca atcccgcttt agctgagcag caagcaacga ttacagctat
     3181 caaaaaagcc ttgcccgcca tagtcagtat tactgtcagc gaaaccacta aacaaaaaat
     3241 tgatggccaa ttagttaatc aaaaaattcg ccgtggctca ggcaccggtt ttattatttc
     3301 cagcgacgga ctgattttga ctaatcgcca tgtggttaat caggcgggcg ataagggtga
     3361 ataccgtatt agatttcaat cgggcaaatc ttattatgct cagctaattg acaaagatcc
     3421 gctcaatgat ttagccatac ttaaaatatt tgacaaaaat ttaccaacta tccaattagg
     3481 caatagtcag gacttggtca tgggaacaac ggttattgcc atcggtaatg ccttgggtcg
     3541 ttatcaaaat agcgccacta aagggattgt tagcggtatt ggccgcaatc tcagtgcctc
     3601 taatgacaat ggtcaagatg aactgctaac taatgtcatc caaactgatg cccaaataaa
     3661 tcctggtaat tcaggcggac cattgattga cctaaacgga cgagtagtcg gtattaatgt
     3721 tgcccgtgat gctagtggtg gctctattgg ttttgccatt ccgattgacg atgccaaacc
     3781 agtcattgat agtgttaaaa aacttggtcg catcgttcga cccaggttag gagtcagtta
     3841 tttgaccata aatgacgatt tggctctaga aaaagacttg ccagtcaatc agggcgcttt
     3901 aatcagtgct aataatgacc agccggcggt cattgctgga tcgccagccg aacgagccgg
     3961 catattgccc ggtgatatta ttttagaaat aaacgcgcaa aagctgaatg aacaaaatag
     4021 tttattacag ataattcaaa aatatcaacc gaatcaaaaa attggtctca agattcaacg
     4081 tggtgataaa aaaataatcg ttcaagccac tttagacgaa tttcctaata gataagtcta
     4141 tgaaaaaaaa gtttgacatt atcactatcg gcggagccac tgaagatctt tcttttctaa
     4201 ttaatgatta tcgtctgctt gataatcgtc gcgaaatttt ggctaaaaaa ttattagcca
     4261 ttgagtatgg agctaagacg attgtagatg agagttttgt agaatatggt ggcggcgcag
     4321 ctaatttggc aatggctatc aagcaatgcg gccttagtgt cgccagctgg ctagctgttg
     4381 gatctgatcg tcgcggtcaa gatattatta ataatctcaa aagccataaa atagataccg
     4441 ggttgtgcca aataattgac cggcagcaat cagccgtgtc ggttgtttta atcggccaag
     4501 acggggagca tacaattatt gcttatcgcg gagccaatga tcatctatca ataacttcat
     4561 cagctgccaa acagctggcc agtgattggc tttatgtttc gtctttaagc ggtaaaatct
     4621 ggttaaaaaa tttaacagcc atttttaaaa gtcataaaaa gattgcttgg aatcctggac
     4681 aagcccaatt gacggccggc tttaaaaaac tgcggccatt tttagaaaaa actgacctat
     4741 taattattaa tcaagatgaa gccttgcaat tattagctag ccatccgttt gataataaac
     4801 agctagccga ctatggccaa aatatgaaaa aacttattca acgtttatgg tgctatggtc
     4861 ccaaaatagt agtcattacc gctgattccc gaggagcttg gtcttatgat ggtcaaacaa
     4921 ctattcatca gccggcatta ccagtcaaaa aaattattga taccactggc gtcggcgatg
     4981 cttttggcgg gactttatta ggttgtctaa tcaaaactaa tagccttaaa aaatctttat
     5041 atctggctgc caaacaggcg gcggcagcta tcagccaacg tggcgctcag tctggattaa
     5101 ttaatatcag taaactaatt cgctaattta tgcctgagtt gcccgaagta cagacgattg
     5161 ttaatgattt gcagccactg ataatgaaca aatatattat acggctgcaa attttagata
     5221 gccgactaaa aaatctaaat actaagccgt cattacaatt aattattggg caacccatta
     5281 ctaatatttt ccgacgtgct aaaaatctag tcattgaact agctaataat tattacttgg
     5341 tcgtccatct taaaatgact gggcaactaa tttggacaag taacgagcag tggctagccg
     5401 gcggacaccc aactacagat ttattgagta gtaaccaagc tagctggcca aataaatcaa
     5461 cccgtttaat tatagaatta tcgaatcatg atcggttatt ttttaatgat agtcgtaaat
     5521 tcggctgggt taaattaatg aataaacaag agtggttaca ataccaaaca cgcttaggaa
     5581 tagaaccgtt aagctctaat ttttcctgga ctgatttgca attaatctta aaaaagaaac
     5641 caacaaccaa aattaagcag gtgctattag atcagcattg tttagccggt attggtaata
     5701 tttatgctga cgagagcctt tttgcggccg ggatcaagcc cagtcgacca gctggcacgc
     5761 taacgatgat tgaaattaag aaactacact catcaatcgt taaaatttta accaccgcgg
     5821 tagcttaccg tggtacctca tttagcgatt atcgtgatgg ccgtggccaa agcggtaatt
     5881 tttcacaaca acttcaagt
//
LOCUS       DAOPAI010000031         5810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_11982_length_5810_cov_5.169592, whole genome shotgun sequence.
ACCESSION   DAOPAI010000031 DAOPAI010000000
VERSION     DAOPAI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11982_length_5810_cov_5.169592"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(371..1192)
                     /locus_tag="PKV49_03235"
     CDS             complement(371..1192)
                     /locus_tag="PKV49_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPL22651.1"
                     /translation="MKKAFTLIELLVVIAVIALLSTLSVVALSSARAKSRDARRLSDI
                     KQIQTALEMYFDSSGNYPTSLTAGSPLSYGSLVFLSKVPSDPISNNQYAYTQTEGGHE
                     YTLDFILETKSADYEPGNYQATSKGILAGGGGSTPPAWQCGDNLVDDGQTYSTVENHN
                     YCLMTASLNALNHTRRCFDDNEKNCTNYGALYGLKEGPDLCPNGWRLPINKDYEHFSF
                     LISGQSFIGYYDGKNYKNDTSYLMLADSYVLNVADQTISTLPADSAVSVRCMKDP"
     gene            complement(1197..2447)
                     /locus_tag="PKV49_03240"
     CDS             complement(1197..2447)
                     /locus_tag="PKV49_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HPL22652.1"
                     /translation="MEQVLDLHLHSRFSRACSANLTLPDLERAGRRKGIDIIGTADFT
                     HPQWFKEISEQLIEIGQSGLYRLAETTDSQIKFLLSTEVALIYKQGDKVRRLHLVIHA
                     PNIEAVRQLNQELISRGLNLRSDGRPILGLTAPALVKICRQIDPQFIVYPAHIWTPWF
                     SIFGSKSGFNKLEECFDEQTEFIYAYETGLSSDPPMNWRFSELDKLILLSNSDAHSLA
                     NLAREANVIDLPDLSYQSFCNILKNRDKQKIISTLEFFPEEGMYHLDGHRQCGFVCSP
                     TKTKKIHGLCPHCRRPLTIGVLSRVEELADRPDGYRPDGAPNFEKLVELDKIIAESLG
                     VKSRNSKKVQEIYEQMLQVFDNELAILRKVPLEEIDRQFGGLLAEAIRRMRQGQLTIE
                     PGFDGQYGTIRLFNPAETRPITLF"
     gene            complement(2454..3455)
                     /locus_tag="PKV49_03245"
     CDS             complement(2454..3455)
                     /locus_tag="PKV49_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPL22653.1"
                     /translation="MRIAIFDIKAGEKAYFNKALAGHELAFSADNLAEDNAKLAKNCQ
                     AVMVFVASRLTAEVLAKMPKLKLIVARSTGFDHIDLKYCQKHGIAVCNVPPYGENTVA
                     EHAFALILSLSRNIHKSYLRTMREDYRVEGLKGFDLQGKTIGVIGAGKIGLHLIKMAR
                     GFDMHVLAYDSRQDHFLAEVLGFKYVSLEELLRRSNIISLHVPDNKFTHHLINRQNIG
                     LIKKGALLINTARGGVVETEALIEALDKKILAGAGLDVIEGENLIMEEKQLAYDQETA
                     NEMLSLVKNHILLSKDNVIFTPHIAFYSQEAIERILEVSVDNLKAFVAGRPQNLVKA"
     gene            complement(3506..4282)
                     /locus_tag="PKV49_03250"
     CDS             complement(3506..4282)
                     /locus_tag="PKV49_03250"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPL22654.1"
                     /translation="MDWLSALILGIVEGVSEFLPISSTAHLRLFSQWLGVVNNDFSKS
                     FDIFIQLGAILAVFVVYLQTLWRRPRIIWKLAAAFVPTAIIGLAAYPVIKGQLMDSFW
                     LMAVTIGLGGLFLLWFERRYQFKQATIKEVDQITYKQAILIGLCQSIAMIPGVSRSAA
                     TIIGGLAIGLSRSVIVEFSFLLALPTMLAATGLDLLKSSWSLGRPEIITLAIGSLAAF
                     ITAWLSVKWLLNFIRQRDFRLFGWYRLALAVIIVIYLLVR"
     gene            4399..5694
                     /locus_tag="PKV49_03255"
     CDS             4399..5694
                     /locus_tag="PKV49_03255"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPL22655.1"
                     /translation="MAQVFLYDEFNPEDTAMLQALYSRSPESVAKHVEKVRQTGSGKF
                     METFYVGYGHSSIADCGSTTLFIEGLSILADKALQDWPLYCGQETSTRYVDMAKQPII
                     DPLNTEQSQKIINDWMNFYKSALEPVKEHLRHSYPRQADHDDKIYQRAINARSFDILR
                     SFLPAGITTQLSWHTNLRQAYDKLTWLRQYPLAEVRQIANQLEQILKEHYPHSFCQTI
                     DDQQENYRHTILANDYYYTTEQPTEFRFTTNIKDNDLNQQTAIINRPPKTGLPHFLAE
                     LGSCSFEFLLDYGSFRDIQRHRNGVCRMPLLTARHGFHNWYLEQLPHDLRQQAIVLIA
                     KQIEAVAALPTDETTKQYYLALGFLVPCKVTYGLPATVYTAELRSNQTVHPTLRQVAH
                     QMIKALQERWPKLQLHADLSPSVWDINRGKQTIEEKTNC"
BASE COUNT         1824 a         1345 c         1037 g         1604 t
ORIGIN      
        1 cgtctctttg agtcaaggca tccaccatag acccttaacg agattttccc acaatcctat
       61 tctcaaggat tgctaaaatc tttattttat tatagacgcc atataatcat cagcgattat
      121 acggctgttt atgctatctg atctaacgat atatttatat tgttaatgtt ctttgacttt
      181 ataagtttag aaaaacttat aacctgtgcc acgcaaccga aaattaaaat tagctgcgga
      241 gcaagagtta taagtctttt aagaggttgg cttaaccaac attaatttta attgtgtttc
      301 ctattataca cgaatagaaa aggctgtcaa gggtttaatt ccaatttaaa atataaataa
      361 taggagtaat ttatggatcc ttcatacaac gaaccgatac ggcgctatcg gcaggcagag
      421 tagaaatcgt ttggtcagca acatttagaa cataagaatc agctaacatt aagtaactgg
      481 tatcattttt ataattttta ccatcatagt agccaataaa agactgtcca ctaattaaaa
      541 aagaaaaatg ttcatagtct ttatttattg gcaaccgcca accattggga cataaatcag
      601 ggccttcttt taaaccataa agagctccat agttagtaca atttttttca ttatcatcaa
      661 aacatcggcg agtatgatta agagcattta aactagctgt cattaaacaa tagttatggt
      721 tctcaactgt cgaatatgtt tgaccatcgt ctactaaatt atcgccacac tgccatgctg
      781 gcggtgtgct accgcctcca ccagctagaa tgcccttgga agtcgcctga taatttcccg
      841 gctcataatc cgccgacttc gtttccagga taaaatccaa agtgtattca tgacctccct
      901 cggtttgagt gtaggcatat tgattattac taattggatc acttggcact tttgataaaa
      961 aaaccaagct gccataagat aaaggagatc cagccgttaa cgatgtcgga taattacccg
     1021 agctgtcaaa atacatttcc agagcggttt gaatctgttt aatatcagac aaacggcggg
     1081 catcccgcga tttagcccga gcgctgctta aagctaccac tgaaagagtg gataataaag
     1141 caataacggc gataaccact aacagttcaa tgagtgtaaa agcttttttc atatctttag
     1201 aataaagtaa ttggtcttgt ttcggccgga ttaaaaagtc taatcgtgcc gtattggcca
     1261 tcaaagcctg gttcaattgt tagctgtcct tggcgcatcc gtctgatggc ttcagccaat
     1321 aagccgccga attgccggtc gatttcttct aatggaacct ttcttaaaat cgccaattca
     1381 ttatcaaaaa cttgtaacat ttgttcataa atttcctgca ctttcttaga gttgcggctc
     1441 tttaccccca aactctcagc aattattttg tctagctcca ctaatttctc aaaattgggc
     1501 gctccgtcag gccgatagcc gtcaggccga tcagccaatt cttcaactcg actcaaaacg
     1561 ccaatcgtta agggtcggcg gcaatgcggg caaaggccat gtattttttt ggttttggtt
     1621 ggcgagcaaa caaaaccgca ttgacgatgg ccgtccaaat gatacatgcc ctcttcggga
     1681 aaaaattcca aggtgctaat aattttttgc ttgtctctat ttttaagtat attacaaaat
     1741 gattgataac tcaaatcagg caagtcaata acattggctt ctcgcgccaa attagccaat
     1801 gagtgagcgt cagaattaga taataaaatt aacttatcca gttcggaaaa acgccaattc
     1861 atcggtgggt cgctagatag gccggtttca taggcgtaaa taaattcagt ctgttcgtca
     1921 aagcattcct ccaatttatt aaaaccggat ttagagccaa aaatcgaaaa ccacggcgtc
     1981 caaatatggg ctggataaac tataaattgt gggtcaatct gacgacaaat tttgaccagc
     2041 gccggcgcgg ttaaccccaa aattggccgc ccatcactgc gcaaatttag accacgacta
     2101 attaattcct gatttaactg acgcaccgct tcaatattag gtgcatgaat aactaaatgc
     2161 agacgacgca ctttatcgcc ctgcttatag attaaagcaa cttcggtact caataaaaat
     2221 ttaatttggc tatcagttgt ttcggctagc cgatataatc cgctctgacc aatctcaatt
     2281 aattgttcac taatttcttt aaaccactgc ggatgagtaa aatcggctgt tccaataata
     2341 tcaatacctt tgcgtcggcc agcccgctca agatcaggca gcgtcaaatt agctgaacaa
     2401 gcgcggctaa aacgagaatg caggtgtaga tctaaaacct gttccatgtt taattaggct
     2461 ttgactaaat tttgtggccg gccagccaca aaagccttaa gattatccac gctgacctcc
     2521 agaatccgct caatcgcctc ttgactataa aaagcaatat ggggggtaaa aataacattg
     2581 tctttgctaa gcaaaatatg attcttaacc aaagatagca tctcattagc tgtttcttga
     2641 tcataggcta actgtttttc ctccataatc aaattctcgc cctcaatcac atccaatccg
     2701 gcgccggcca aaatcttttt gtctaaagcc tcaattaggg cctcagtttc caccactcca
     2761 ccgcgagctg tattgattaa taaagcccct ttcttgatta aaccaatatt ttgtcggtta
     2821 attaaatgat gagtaaattt attatccggt acatgtagac tgataatatt tgatcgcctt
     2881 aatagttctt ctaaagaaac atacttaaag ccaagcactt cggctaaaaa atgatcttgg
     2941 cgcgaatcat aagccaagac atgcatatca aaaccacgag ccattttaat taaatgcagg
     3001 ccaattttac cagcaccgat aacgccaatg gttttaccct gcaaatcaaa acccttgagt
     3061 ccctcaaccc gataatcctc gcgcatcgtc cgtaaatacg atttatgaat attgcgcgac
     3121 aagctaagaa tcaaggcaaa agcgtgttcg gccacggtat tttcaccata aggcggcaca
     3181 ttgcaaacag caatgccatg tttttggcaa tactttaaat caatatggtc aaaaccggtg
     3241 gaacgagcaa caatcaattt taatttcggc atcttagcta atacctcggc cgtcaatcgg
     3301 gaagccacaa aaaccattac ggcctgacaa tttttagcta acttagcatt atcttcagcc
     3361 aaattatcgg cgctgaaagc taattcatgg ccggctaacg ccttgttgaa atatgctttt
     3421 tctccggctt taatatcaaa aatggcgatg cgcataaaat tccaatttat tttttatttt
     3481 aatattataa caaaaatagc ctaatttagc gaactaataa atatataaca ataataactg
     3541 ctaaagcgag ccgataccag ccgaacaatc gaaaatcacg ctggcggata aaattgagca
     3601 accactttac acttaaccaa gcagttataa aagccgccaa tgaaccaatc gctaaagtaa
     3661 taatctctgg ccgccctaac gaccaagaac tcttcaatag atctaagccg gtcgctgcca
     3721 acatggttgg tagggctaaa agaaaactga attcaacaat tactgaccgg cttaagccaa
     3781 tagccaaacc gccgataatc gtcgccgctg aacgagacac gcccggaatc atggctatag
     3841 actgacacaa accaattaaa atagcctgtt tataagttat ttgatcaacc tctttaatcg
     3901 tggcctgttt aaattgataa cgccgctcaa accaaagcaa aaacaaacca ccgaggccaa
     3961 tggtcacggc catcagccaa aaactatcca ttaattgacc cttaatcaca ggataggcag
     4021 ctaatccaat aatcgctgtc ggcacaaaag ccgcagccaa tttccaaata atccgtggtc
     4081 gccgccataa tgtttgtaaa taaaccacga ataccgccaa aatagcccca agctgaataa
     4141 aaatatcaaa actcttgcta aaatcattat taaccacccc gagccattga ctaaataatc
     4201 gcaaatgagc agttgaagaa atcggcagga attcgctaac gccctccaca atacctaaaa
     4261 ttaaagccga caaccaatcc ataataataa attaactaat tattaccttt tattgtaaag
     4321 caattcggcc gattggccaa gctttgactg agtcgctcgc ggctgataca ataaacttac
     4381 cattcttaaa ttaatcctat ggcccaagtt tttctttatg atgaatttaa tcccgaagat
     4441 acagcgatgc ttcaagcgct ttattcgcgc agcccggaaa gcgtcgccaa gcatgttgaa
     4501 aaagttcgcc agaccggatc tggcaaattt atggaaacct tctatgtcgg ctatggccac
     4561 tccagcattg ccgattgcgg cagcaccact ctttttattg aaggcttaag tattttggct
     4621 gataaagcct tgcaagactg gccgctctat tgtggccaag aaaccagcac tcgctatgtt
     4681 gatatggcta aacaaccaat tattgatccg ctcaacacag aacaatcaca aaaaattatc
     4741 aatgattgga tgaatttcta taaatcggcc ctggaaccgg tcaaagaaca tttacgccat
     4801 agctatcctc gtcaagccga tcacgacgac aaaatttatc agcgcgctat caacgcccgc
     4861 tcttttgata ttttacgctc ttttctcccg gccggtatta ctacgcagct tagctggcat
     4921 accaacttac gtcaggctta tgacaaattg acttggttac gccaatatcc cttagccgag
     4981 gttcgccaaa ttgccaacca gttagagcaa atcttaaagg aacattaccc gcacagtttt
     5041 tgtcaaacaa ttgatgatca gcaagaaaat tatcgtcaca ctattctggc caatgactat
     5101 tattatacta ccgaacaacc gactgagttc cgttttacca ccaatattaa agataatgat
     5161 ttgaatcaac agacagcgat tattaatcgc ccgcctaaaa ccggcctacc ccacttctta
     5221 gctgaattag gcagctgtag ttttgaattc cttttagatt acggttcttt ccgcgatatt
     5281 cagcgccatc gcaacggcgt ttgccggatg ccattattaa ctgccagaca cggttttcat
     5341 aattggtatt tagaacaatt accccatgat ttgcgccagc aggctatagt gttaatcgcc
     5401 aagcaaattg aggctgttgc cgctctaccg actgacgaaa cgactaaaca atactattta
     5461 gctctcggct ttttagtgcc ttgcaaagtc acttacggtc taccggctac agtttatacc
     5521 gctgaattgc gctctaacca aaccgttcac cccactttgc gccaagtggc tcatcagatg
     5581 attaaagcct tgcaagagcg ttggcccaaa ctacagctcc atgccgatct atcgcctagt
     5641 gtttgggata ttaatcgcgg taaacaaacc attgaagaaa aaactaattg ttaaaataaa
     5701 aagaggcgca atgagcctct ttttaagcat atacaaaaaa cgccccataa tataggcgtt
     5761 ctgcggagtc gacgggactc gaacccgcga cctctgccgt gacagggcag
//
LOCUS       DAOPAI010000032         5520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_12654_length_5520_cov_4.988472, whole genome shotgun sequence.
ACCESSION   DAOPAI010000032 DAOPAI010000000
VERSION     DAOPAI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12654_length_5520_cov_4.988472"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PKV49_03260"
     CDS             complement(<1..180)
                     /locus_tag="PKV49_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22656.1"
                     /translation="MPYSWWQTIKDKQSIIWWWLVGLLAAAILVSFLQPLKYSAESQL
                     LVVPDYQQTSDPYQIS"
     gene            complement(298..1227)
                     /locus_tag="PKV49_03265"
     CDS             complement(298..1227)
                     /locus_tag="PKV49_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPL22657.1"
                     /translation="MEGQNYDLLIIGAGPAGLTASIYASRYKIKHAVLGSLPGGTITE
                     AHKICNFPSEANINGWDLAQKIESNARQLGAEFMTSEAVAISGQYPNFAVELASGQVL
                     LTKSILLATGTKRRHLGLDNEQQYLGKGLAYCATCDGPFFKDKLVGVLGGGNSAATAA
                     LYLADIARQVYWFYLGDRPEAAEPSWLEEIDKRANIKHYPNVKVNQLLGDNHLTGVEL
                     INSDHQTSQLNIDGLFIEIGSDPATGLLQQLNGQINDRGYAIVDAGQATTVAGVWAAG
                     DATSNSNGLRQIITACSEGAIAADSIFRFLKKN"
     gene            complement(1229..1561)
                     /gene="trxA"
                     /locus_tag="PKV49_03270"
     CDS             complement(1229..1561)
                     /gene="trxA"
                     /locus_tag="PKV49_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPL22658.1"
                     /translation="MSQVLELNPGNFAAAVLQSELPVLVDFWAPWCGPCRMMAPVLDS
                     LAAELAGKVKIAKINVDDPANQPLAAQYGIQGIPNLKLFKAGHEIADFVGMRQLDSLR
                     QDILAKIA"
     gene            complement(1602..1862)
                     /locus_tag="PKV49_03275"
     CDS             complement(1602..1862)
                     /locus_tag="PKV49_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPL22659.1"
                     /translation="MATNFFAGTRTPEQRLNIIIGQLNGLKKTLAKGRYDCYALITQI
                     KATKAGLDAVLEKILEEQLDRCLKTAKSKPEVKKIFSQVVRK"
     gene            complement(1893..3911)
                     /gene="mrdA"
                     /locus_tag="PKV49_03280"
     CDS             complement(1893..3911)
                     /gene="mrdA"
                     /locus_tag="PKV49_03280"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPL22660.1"
                     /translation="MARDNANNPFSDDLGAISHRRKALRLSSTNHRWTEEVFLSQTDN
                     NETISHNFSNTRGYIFSQICLAALLLLLARLIWLQLYRGDYYYNLAEGNRLRLERLEA
                     KRGIIYDSQRQPLVQNVANFLVYVTPSNLPSKPVDRQAVLARLAAVLGEEVSVKLESD
                     LATIQPRSLDFYQPIFVSDNLTYDQAVQLTVQAHQLPGVVLTNRNKRQYLPISSFLGT
                     SSQPFDSDSLSLIIGYIGKINEQELSKNKADNYLPIDYIGKVGLEAYYEKDLRGLSGS
                     KQIEVDAVGNEKKKIAQQNPLDGRHLVLNIDSRLQAKLEEIVRQHLDRVNSRKAVAIA
                     LDPNNGAILAMVNQPSFDNNDFANGIDQATYQKLINDPAKPMFNRAVSGEYPSGSTVK
                     IVMAAAALQEKVITATTGFMSTGGLRISRWFFPDWKSGGHGWTDVSKAIAESVNTFFY
                     YIGGGYEQFNGLGLEKIVKYLQLFGLGKTTGIDLAGEASGFIPTAAWKQEKTGEPWYI
                     GDTYHLAIGQGYLLVTPLQVANYTMAFANGGRIYQPRLVKEILNSDESVYQTMVPKTM
                     SQDIVNPDNLQTVRQAMRQTVLTGSARSLQSLPVPVAGKTGTAQFDSRKATHSWFTGF
                     APFNQPKIVLTILVEEGGESTDAAIPIAKDFWQWYFSSIDTPEGYSVK"
     gene            complement(3915..4415)
                     /locus_tag="PKV49_03285"
     CDS             complement(3915..4415)
                     /locus_tag="PKV49_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22661.1"
                     /translation="MGWLQLFNALWLLLVALIQLSWIDQLPIIWLRPNLLLVILINWL
                     ALSGWRRVNWWVIFLGLFLDCLAFYPFGSQTLLWMLTMGLVNLLLVNFFTNKSLTAFS
                     GLVVIATIFQWLGRIIISRHWPVNQWWLDLGGQILANWLLMVIIHYLIYWLDRRLNPL
                     FLFKRR"
     gene            complement(4420..5238)
                     /gene="mreC"
                     /locus_tag="PKV49_03290"
     CDS             complement(4420..5238)
                     /gene="mreC"
                     /locus_tag="PKV49_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPL22662.1"
                     /translation="MAIIRLPGKKSSAWPWLLLVMLVLAHFIGLLTGVENMVGRAVQP
                     VLRTLSSRNQSLTGWLNNWRERRACQTELSEVKNQNSRLTAQTISLASLVEENKELRQ
                     LLNFQKAREFKLVTANIVYRGQSGGLLAPSQTLIIDQGARQGIATDLAVIDQQGAIVG
                     KIGQVKDNSAEVLLVTNKNCLLAAATASSSQTIGVVRGEMGLTIRLEMVAQNEPLQVG
                     QDIVTSGLEPAVPPNLLIGRINQVNKENNALWQDAGLEPLTDANQLNFVSVIIP"
     gene            complement(5242..>5520)
                     /locus_tag="PKV49_03295"
     CDS             complement(5242..>5520)
                     /locus_tag="PKV49_03295"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPL22663.1"
                     /translation="AIARPISQIIDNIKATLEVTPPELVADIYERGIMLAGGSSLLAG
                     FDQAIARATKIGVHIVDEPISSVVRGAAHLLTDTNLLDKVVAPSLTDL"
BASE COUNT         1633 a         1337 c         1048 g         1502 t
ORIGIN      
        1 agagatctgg taaggatcac tggtttgttg gtaatcaggc actaccagta attggctttc
       61 agctgaatat tttaaaggtt gcaaaaaact aaccagtata gccgcagcta atagaccgac
      121 taaccaccac caaatgattg attgtttatc tttaattgtt tgccaccaag aataaggcat
      181 tggaaatcaa tgaaaaatta attgattaac tagtttaaca tagttatcat tttttgtcaa
      241 gagtaagacg ccgcccttaa agagcggctt ctggcaaaat taaaaaataa tctaggctta
      301 attctttttt aaaaatctga agatactatc ggcggcaatc gccccttctg agcaagcagt
      361 aatgatttgt cgcaagccgt tagaattgct tgttgcatca ccggccgccc aaactccagc
      421 tacggtcgtg gcttgtccgg cgtcaacgat ggcatagccg cgatcattaa tttgtccgtt
      481 caattgctgt aataatccgg tagccggatc gctgccaatt tctataaaca aaccgtctat
      541 gtttaattgg ctggtttggt ggtcagagtt aatcaactct actccggtta aatgattatc
      601 acccagcagc tgattcacct taacattggg ataatgctta atattggcac gcttatctat
      661 ttcctccagc cagcttggct cggctgcttc agggcgatca cccaaataaa accaataaac
      721 ttgtcgagct atgtctgcta aatatagggc tgctgtggcc gcgctattac caccaccgag
      781 caccccaacc aatttatctt taaaaaatgg accatcgcag gtggcgcaat aggccaaacc
      841 cttgcctaag tattgctgtt cattatctag tcccaagtgg cggcgtttag tgccagtagc
      901 caataaaata gatttagtta ataaaacttg accgctagcc agttcaaccg caaaattagg
      961 atattgtccg ctaattgcta ccgcctcgct agtcataaat tcagcgccca attgacgagc
     1021 attgctctca atcttttgcg ccaaatccca accgttaata ttggcttcac taggaaaatt
     1081 acagatctta tgggcttcag ttatggtacc gcctggcagg ctgccgagca cagcgtgctt
     1141 aatcttgtaa cggctggcat agatagaggc agtcaagccg gccggaccag ctccaataat
     1201 taataaatcg taattttggc cttccatact aagctatctt cgccaaaata tcctgacgca
     1261 aactatctag ttggcgcatg ccgacaaaat cggcaatctc gtgaccggcc ttaaataatt
     1321 tcaaattagg tatgccttga atgccgtatt gagcagctaa cggctgatta gctgggtcat
     1381 caacattgat cttggcgatc ttaacctttc ccgctaactc ggcggccaag ctatccaaaa
     1441 ccggcgccat catccggcac gggccgcacc acggcgccca aaaatccact aaaactggca
     1501 actcactctg taaaaccgcg gcggcaaaat tacccgggtt taattctaaa acctgtgaca
     1561 taagctatcg ttaattttat tagcaaaata ccaaccaata attatttgcg cacgacctgg
     1621 ctaaaaatct ttttaacctc gggcttgctt ttggctgttt tgaggcaacg atccagctgc
     1681 tcttccaaaa tcttttctaa aacagcatcc agccccgctt tggtggcttt aatctgggta
     1741 attaaagcat aacaatcgta gcggcctttg gctaatgttt tctttaggcc atttaactgg
     1801 ccaataataa tatttaaacg ctgttccggt gttctagtac cggcaaaaaa attagttgcc
     1861 ataataatta tttaaatttt taaaacttat ttttatttta cactataccc ctctggggtg
     1921 tcaatagagc taaaatacca ttgccaaaaa tccttggcga tcggtatcgc ggcatcagtg
     1981 ctttcaccgc cttcttccac taaaatcgtt agcactattt ttggctgatt aaacggcgca
     2041 aagccggtaa accaagaatg ggttgccttg cggctgtcaa attgggctgt gccagtctta
     2101 ccggctaccg gtaccggtaa gctctgcaaa ctgcgcgcgc tgccggtcag caccgtctga
     2161 cgcatggctt gacgaacagt ttgtagatta tctggattaa cgatatcttg actcatagtt
     2221 ttcggcacca tagtttgata gaccgactcg tcgctattaa gaatctcttt aaccaatctg
     2281 ggctgataaa tccgtccacc attagcaaaa gccatagtat aattagccac ctgcaatggc
     2341 gtcaccaata aatacccttg gccaatagct aaatggtaag tatcgccgat ataccaaggc
     2401 tcgccagtct tttcttgttt ccaggccgcc gtcgggataa aaccgctggc ctcgccggcc
     2461 aagtcaatac cggtagtttt accaagacca aataattgca aatatttaac tattttttct
     2521 agacccaaac cattgaattg ttcataaccg ccgccaatat aatagaaaaa ggtattaacc
     2581 gattccgcaa tcgctttgga cacatccgtc cagccatggc caccactctt ccaatcagga
     2641 aaaaaccacc ggctaattcg taatccgcca gtactcataa aacccgtagt ggcggtaatc
     2701 actttctctt gcaaagcagc ggccgccata acaatcttaa ccgttgaacc cgatggatac
     2761 tcgccgctaa ccgcccgatt aaacattggc ttggctggat cattaatcag cttctgatag
     2821 gtcgcttgat caatgccgtt ggcaaaatca ttgttatcaa aactgggctg gttaaccatg
     2881 gctaaaattg cgccattatt gggatctaag gcaatggcta cagccttacg gctattaacg
     2941 cggtctaaat gctgacgaac tatttcttcc aatttagcct gtaagcgact atcaatattc
     3001 aaaactaaat ggcggccatc taacggattc tgctgagcga tttttttctt ttcattacca
     3061 acggcatcaa cctcaatctg tttgctaccg cttaggccgc gtaaatcttt ttcataataa
     3121 gcctccaagc caactttgcc gatataatca attggtaaat agttgtcagc cttgtttttg
     3181 cttaattctt gttcattaat tttgccgata taaccaataa tcaagctcag gctatcggaa
     3241 tcaaatggtt ggctggaagt gcccaagaat gagctgattg gtaaatactg gcgtttattg
     3301 cgattggtta acacaacccc cggcagctgg tgcgcctgca cggtcaactg gaccgcttga
     3361 tcataagtta aattatcgct gacaaaaatg ggttggtaaa aatctagtga tcgcggttgg
     3421 attgtcgcca gatcactctc caattttaca ctaacctctt cacccaacac tgcagccaga
     3481 cgagccaaga ccgcctgccg gtctaccggt ttactaggca agttactggg tgttacatag
     3541 actaaaaaat tagccacatt ttgcactaac ggctgccgtt ggctgtcata aataatcccc
     3601 cgcttggcct ccaaacgctc caaacgtaat cgattgcctt cagccaaatt ataataataa
     3661 tcaccgcgat aaagctgtag ccatattaaa cgagccaaaa gcaataataa tgccgctaaa
     3721 caaatctgag aaaaaatata gccacgagtg ttagaaaaat tatgactaat ggtttcatta
     3781 ttgtcagtct ggcttaaaaa aacttcctcg gtccagcgat ggttagttga agatagtcgt
     3841 aaagccttcc gacgatgact aatcgccccc aggtcgtcgg aaaatggatt gttggcatta
     3901 tctctggcca taaattagcg tcgtttgaat aaaaacaatg gattcagccg ccgatctaac
     3961 caataaatta aataatgaat aatgaccatc aacaaccaat tagctaaaat ctgtccgcct
     4021 agatcaagcc accactgatt aaccggccag tggcgactaa taattattcg tcccaaccac
     4081 tgaaaaattg tggcaataac caccagcccg gaaaaagctg tcaatgattt attagtaaaa
     4141 aaattaacca ataataaatt caccaagccc atcgtcaaca tccaaagcag agtttgacta
     4201 ccaaacggat aaaaagctag acaatctaaa aataagccta aaaatattac ccaccaatta
     4261 actcgccgcc agccactcaa agctaaccag ttaattaaaa ttaccaataa caaattcggt
     4321 cgcaaccaaa ttattggcaa ctgatcaatc cacgacagct ggataagagc cactaataac
     4381 aaccacaggg cattgaacaa ttgcagccaa cccataagct taaggaataa taacgctaac
     4441 aaaatttaac tgattggcat cggttagcgg ttctagccca gcatcctgcc ataaagcatt
     4501 gttttcttta ttgacctgat tgatccgacc aatcaataga ttgggcggca cagccggctc
     4561 taatcctgaa gtgacaatat cttggccaac ttgtagcggt tcattttgcg caaccatttc
     4621 cagcctgata gtcagcccca tctcgccacg gacaacgcca atagtttgac tactactagc
     4681 tgtggccgct gctaaaagac aatttttatt ggtgaccaac aaaacttcgg ctgaattatc
     4741 cttgacctga ccaattttac cgacaatagc cccctgctga tcaataaccg ccaaatcagt
     4801 cgctattccc tgtcgagctc cttgatcaat gattagagtc tggcttggcg ctaataatcc
     4861 accagattgg ccgcgataaa caatattggc tgttactaat ttaaattccc gagctttttg
     4921 aaaatttaaa agctgacgta attctttatt ctcttcaact aaactggcta aactgatggt
     4981 ttgcgccgtt agccgactat tctgattctt aacttctgat aactctgttt gacaggcccg
     5041 tcgttctcgc caattattca gccaaccagt taatgattga ttgcgagatg agagtgttct
     5101 aaggactggc tgaaccgctc gtccaaccat attttctact ccagttaata agccaataaa
     5161 atgcgctaaa accagcatga ccaataataa ccatggccag gctgacgatt tcttaccagg
     5221 caaacgaatt atggccataa tttataaatc agttaagctc ggagctacca ctttatccaa
     5281 tagattagta tcggttagca gatgggctgc cccgcgaaca acgctgctga tgggctcatc
     5341 aacaatatga acaccgattt tagtagctcg ggcaattgct tgatcaaagc cggctaataa
     5401 tgagctgcca ccagctagca taatgccccg ttcataaata tcagcgacta attccggcgg
     5461 cgtaacctct aaagtcgctt taatattatc aataatctgg ctaattggcc gagcaatagc
//
LOCUS       DAOPAI010000033         5366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_13061_length_5366_cov_3.746940, whole genome shotgun sequence.
ACCESSION   DAOPAI010000033 DAOPAI010000000
VERSION     DAOPAI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13061_length_5366_cov_3.746940"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..596
                     /locus_tag="PKV49_03300"
     CDS             168..596
                     /locus_tag="PKV49_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HPL22664.1"
                     /translation="MKILIAPLAAAAIAQFSKVFIKSNKQKLSWSTLWVYSGMPSGHA
                     AMTVALTTIIGLEKGLDYPGFAIALVFTLLIIRDAVGLRRQLGNQGSVLNDLVDDLED
                     DNYLDKRYPQLLEKIGHTPTQIAVGALIGLVVAIFIYYFS"
     gene            complement(589..1254)
                     /locus_tag="PKV49_03305"
     CDS             complement(589..1254)
                     /locus_tag="PKV49_03305"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22665.1"
                     /translation="MKQMEQTTKYRWPIIISSIILLIIVGLVVWRLFLPKKSPVELTN
                     NNENINSQPLNKQPDFKTADDLDGDGLSNAAEQEFGSNPEKADSDGDGLSDWEEKSLG
                     TDPNKVDSDGDGISDYDEYMSLSNPLGEGDLDSDNDGLSDKQELELGSNPYSVDTNQD
                     GINDAEAHRQGISLTDDDLDKDGVTNFEEEIRGTNPRKADSDGDGIDDYQQIFGRPRP
                     QVN"
     gene            complement(1264..5184)
                     /locus_tag="PKV49_03310"
     CDS             complement(1264..5184)
                     /locus_tag="PKV49_03310"
                     /inference="COORDINATES: protein motif:HMM:NF027795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5057 domain-containing protein"
                     /protein_id="HPL22666.1"
                     /translation="MPTHSRFLFASLITLVLASLTLPALAANNLLAVEGLRLINTDTT
                     NRITSSTQTVPSVSPDAIAIRIFDNANNLSPRTWYTQNIGAKRSLQSLLVDGYDAVRD
                     NRTVYVAAANITSCQSISAESNCLKPIMVIISFNQNISNETVDIFGQILANWRFNQNI
                     NSQPGVCSNSGGNLSCLSDRDCQSGQICDSLKARVVRDTKRLIDLSAMKTKLEQYRQT
                     YQRYPSLESGTYLVNKTLSVWPSWQKTLAVKMKTALPVDPINRLGTSPSGYNQITGWN
                     EQQRSFATTWPTLPENSRVYQYELINNTRYTLCANFETNYQNITNYACANNYTNQGPV
                     INCPVIRGTAGQAFSAYAEIFDNEGDTINSIINHNQLSGWSHLNVQLVNSGRQLKISA
                     ATAGPDGRYDLILNTSDSLGATSTKTCSIIIGHGLCGNNLIDPNEDCEGDLGVAANAH
                     DSSPNKQYSCNQYCKFTGGWCGDNIIQNGQNFTVNREQCDGNNGIATKTNDSGPNRQY
                     ACHESISPNACTFTGGYCGDHIIQTSANTQINEDCDRPIDSPNPDPSSTYNGIQRYQC
                     GQPGTVNACKFTGGWCGDAVVDYDYGETCEGSTYTVPTPANSSSTKQYACSATTCQVT
                     DGWCGDGQIKDGIENCDCANNQKDCAWAGNSVNPDPSATSSPTNPHRCKNCLQTGGYC
                     GDGIVQEQSEKCDPLEPLSVFKSRTGNLNIDQAYYDQFIAVCLTQNCQTSCYDQDGDG
                     YGIKKYTNCTNTAVDCEDRPNGADNLANSADDGININPGQPDNCTQYDGLDNDCNGYI
                     DDKAWLYTSPLWFDNFARGIFNHLGPAVATNGSFAKSTIDPTTSAGGDPKSAKMVQDR
                     QSAAPNYIWPNVCNNLNYTSNSNCTAYGGKSLTGLTWNITNIGLNINQSYVLRFKYKG
                     SANFLHPTTTNPTNQIHWSIGNNPSINYSYLVTSTSNLIVPGNYTSFHTRPYLGSFTY
                     YQNLNQLANNLYLSIFIGQNNLPNDGNKLNIDDVSLTSCQNIRSDNTYCGNGVIEGAY
                     ETCDFNMRPPNWDDATEFCDDSCQKGRRLKILQVHPGNNNLIDLQNIINNNKNNYITK
                     TATITAVSLADFNASPATYIPNSNPTSSYNVIVFGFQDCNGSGDGTDQLAGGRDLSPN
                     AASSTERFINAGGGVIFGHDTIWQESGTICGGSHNNFNRLAGYAGISLANYQNTSYNY
                     NNISKNINQASTPASALMFNKPYIMDDSFVVNFTHTSGSLPNPSLCSSADGLKIWFHG
                     GDINTKFWATTCGRVEHIMLGHTGSINDNEQKALINMLYYVATH"
     gene            complement(5254..>5366)
                     /locus_tag="PKV49_03315"
     CDS             complement(5254..>5366)
                     /locus_tag="PKV49_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HPL22667.1"
                     /translation="LQVYGRSKQNCLVCGQPLNKIKLAGRGTHFCPNCQK"
BASE COUNT         1500 a         1036 c         1116 g         1714 t
ORIGIN      
        1 attgcttagg attattctga cggctgaacg gccaaagacg tgttcctaac cctccagctc
       61 ggataactat tttcataatt taggattatg attgactaat tgtattatag ccgattaatt
      121 atctttttgc tataatgaaa aaaacttaaa tataaaattt taagtctatg aaaatattaa
      181 tcgctccctt agcagctgca gctatagccc agtttagtaa agtttttatt aaaagcaata
      241 agcagaagtt atcttggtcc accttatggg tttattcggg tatgccatcg ggtcacgcgg
      301 caatgacggt agccttgacc actattattg gcttggaaaa gggtttagat tatccgggct
      361 ttgctattgc gctagttttt actttactaa ttattcgtga tgcagttggc ttgcgccggc
      421 aattaggtaa tcagggatcg gttttaaatg atttagttga cgatttagaa gatgataatt
      481 atttagacaa acgctatccg caattgctgg aaaaaattgg tcacactcca acgcaaattg
      541 ctgtcggagc gttaattggt ctagtggtag caatttttat ttactatttt agttgacttg
      601 tggtcgtggg cgaccaaaga tttgttgata atcatcaatg ccgtcgccgt cgctatcagc
      661 tttacgagga ttagttccgc gaatttcttc ttcaaaatta gtaacgccgt ctttatctaa
      721 gtcatcatcg gtcagactga taccttgccg gtgtgcttca gcgtcattga taccatcttg
      781 gttggtgtcg accgaataag gattggagcc tagctctaat tcttgtttat cgctcagacc
      841 gtcgttatca ctgtctaaat caccttctcc aagtggattg cttaagctca tgtattcatc
      901 ataatcgcta atgccgtcgc cgtcgctgtc gactttattc ggatcggtgc ctaatgattt
      961 ttcttcccaa tcacttaagc cgtcgccgtc gctgtcagcc ttttctgggt tgctgccaaa
     1021 ttcttgttca gccgcattac ttaaaccatc accatctaaa tcatcggccg ttttaaaatc
     1081 aggctgtttg ttcagtggct gactgtttat attttcatta ttattggtca attcgactgg
     1141 tgattttttg ggtaaaaata gccgccagac aacaagaccg acgattatca acagaataat
     1201 cgaggaaata ataattggcc agcgatattt agtagtttgt tccatttgct tcatagttac
     1261 ttattaatga gtggctacat aataaagcat attaatcaga gccttttgtt cattgtcgtt
     1321 gattgatcca gtatgaccaa gcattatatg ctctactcgt ccgcaagttg ttgcccagaa
     1381 tttagtatta atatcgccgc catgaaacca tatttttagc ccatcggcac tagaacataa
     1441 agacggattt ggtaatgatc cgctagtgtg tgtaaaattc actacaaaag aatcatccat
     1501 tatatatggc ttattaaaca ttagggcaga ggctggcgtt gatgcttggt taatattttt
     1561 gctaatatta ttataattat aacttgtatt ttgatagttg gccagtgaga tgccggcgta
     1621 accagctaat ctgttgaaat tattatgact accaccacaa attgttcctg actcttgcca
     1681 aatagtatca tggccaaaaa taacaccacc accggcattg ataaatcttt ccgttgaact
     1741 ggcggcatta ggcgacagat cccttcctcc agccagttga tcagtgccgt cgccgctacc
     1801 attacagtct tgaaagccga aaacaataac attatatgaa ctagttggat tgctgttagg
     1861 tatataagtg gctggtgagg cattaaaatc agctaaagag acagccgtga tggtggcggt
     1921 tttggtaata taattgtttt tattgttatt aatgatattt tgtaaatcaa ttaaattatt
     1981 attgcccgga tggacctgca aaatttttag acgacgtcct ttttgacagc tatcatcaca
     2041 aaattcagtg gcatcatccc aattaggggg tcgcatatta aaatcacagg tctcataggc
     2101 tccttctata acgccattac cgcagtaagt attgtcagac cgaatatttt gacaggaggt
     2161 taatgataca tcatcaatat tcaatttatt accatcgtta ggtaaattgt tttggccgat
     2221 aaaaatagat aaatataagt tattagctaa ttggttaagg ttttgataat aagtaaaact
     2281 acccaggtat ggtctggtgt ggaatgatgt atagttgccc gggacaatta aattactagt
     2341 actagttact aaataactat aattgatgct tggattatta ccgatactcc aatggatttg
     2401 attagttgga ttggtggttg tcggatgtaa aaaattagcc gagcctttat atttaaatct
     2461 cagaacataa ctttggttaa tatttaatcc aatattagta atattccaag ttaagccggt
     2521 taaagattta ccaccatagg ctgtgcagtt gctattgctg gtatagttaa gattattaca
     2581 aacattcggc caaatgtagt tgggcgctgc tgactggcgg tcttgcacca ttttagctga
     2641 tttagggtcg ccgccagcac tagtcgtggg atcaattgtg ctcttagcaa agctgccatt
     2701 agtggccacg gcgggaccta aatggttaaa aataccacgg gcaaaattgt cgaaccaaag
     2761 cggactagta tagagccatg ccttgtcgtc gatgtagccg ttgcaatcat tatccaagcc
     2821 atcatattgc gtgcaattat ccggttggcc tggattaata ttgataccgt catcagcgct
     2881 attagctaga ttatcggcac catttggtct gtcctcgcaa tcaacagctg tattggtgca
     2941 gttagtgtat tttttgatgc cgtagccatc gccatcttga tcataacaac tagtttgaca
     3001 attttgcgtt agacaaacag cgataaattg gtcgtagtaa gcttgatcta tattaagatt
     3061 accagttctt gatttaaaaa cggagagagg ttccaacggg tcgcattttt cagattgttc
     3121 ctgaacgatt ccatcgccac aataaccgcc ggtttgtaaa cagtttttac aacgatgggg
     3181 attggttggc gagctggtgg ctgagggatc gggattaacg ctattaccag cccaagcgca
     3241 atccttttga ttattggcac aatcgcaatt ctcaatacca tctttaattt ggccatcacc
     3301 gcaccaaccg tcagtaactt gacaggtggt ggctgaacag gcgtattgct tagttgaact
     3361 agaattagct ggagtgggca cagtataagt gcttccttcg caagtttcac cataatcata
     3421 atcaactact gcgtcaccgc accaaccgcc ggtaaatttg caggcgttga ctgtacccgg
     3481 ttgaccgcat tggtaacgtt gaatcccgtt ataggttgag ctggggtcag gattgggact
     3541 atcgattggc cgatcacaat cttcgttaat ttgggtattg gcactagtct gaataatatg
     3601 gtcgccacaa taaccgccag taaatgtaca ggcgtttgga cttattgatt catgacaggc
     3661 atactgtcta ttgggaccag aatcattggt cttagtggct attccgttgt tgccatcgca
     3721 ttgttcgcgg ttaacagtaa aattttgtcc gttttgaata atattatcac cgcaccagcc
     3781 accggtaaat ttacagtact gattacagct atactgctta ttaggactag aatcatgggc
     3841 attagctgct acacccaaat caccttcaca atcttcattg ggatcaatta aattattacc
     3901 acaaagtcca tggccaataa ttatggaaca agttttagtg ctagtggcgc ccaggctatc
     3961 actagtattt aatattaaat cataacgacc atctggtccg gcggtggcgg ccgaaatttt
     4021 gagttgtcgg ccggaattaa ctaattgaac gtttaaatga ctccaaccac ttaactgatt
     4081 atggttaatt atagaattga tagtatcgcc ttcattatca aaaatttcag cataagcaga
     4141 aaaagcttgt ccggccgtgc ctcttattac cgggcaatta attaccggcc cttgattagt
     4201 atagttatta gcgcaggcat aattggtaat attttggtaa ttggtttcaa aattagcaca
     4261 taaagtatag cgggtattat taattaactc atattgatag actcggctgt tttcgggtag
     4321 tgtcggccaa gtggtagcaa aagatcgttg ttgctcgttc cagccggtta tttggttgta
     4381 accgctagga gaagtaccaa gacgattaat tgggtcaacc ggtagggctg ttttcatttt
     4441 aacagccaag gttttctgcc agctcggcca aactgataat gttttgttga ccaaataagt
     4501 tccagactct aggctgggat aacgctgata agtttgtcgg tattgttcta atttggtttt
     4561 cattgctgat aagtcgatca atcgtttggt gtctcgaacc actctagctt tcaagctgtc
     4621 acaaatttgt ccagactggc aatctcgatc agataaacaa ctaagattgc cgccgctatt
     4681 agaacaaaca ccgggctggc tattaatatt ttgattaaag cgccaattag ctaggatttg
     4741 cccaaaaata tcgaccgttt cattagaaat attttggtta aaggaaataa tgaccatgat
     4801 tggttttaga caattcgact cagcgcttat actctggcaa gaagttatat tagcggcagc
     4861 cacataaacc gttcgattgt ctctcaccgc atcataacca tctactaata gcgattgtag
     4921 tgatcgctta gctccaatat tttgagtata ccaagttcga ggactgagat tattagcatt
     4981 gtcaaagatt ctgatggcaa tagcatccgg actgaccgat gggacggttt gggtgcttga
     5041 agttatccta ttggtagtat cggtatttat taatcttaat ccctcaactg ccagcagatt
     5101 gttggctgcc aaagccggca atgttaaact agccaaaact aaggttatta ggctagcaaa
     5161 taagaagcgg gagtgggttg gcatatagct tatttttgtt tatatttatc ttacatatta
     5221 tagcaaatag ccaattattg gccaagcttt agattatttt tgacaattgg gacaaaaatg
     5281 agttcctcgg ccagccagtt ttattttatt tagcggttgt ccgcaaacta gacaattttg
     5341 tttggagcgg ccatagactt gaagtt
//
LOCUS       DAOPAI010000034         4572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_15672_length_4572_cov_6.209652, whole genome shotgun sequence.
ACCESSION   DAOPAI010000034 DAOPAI010000000
VERSION     DAOPAI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15672_length_4572_cov_6.209652"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3052)
                     /locus_tag="PKV49_03320"
     CDS             complement(<1..3052)
                     /locus_tag="PKV49_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22668.1"
                     /translation="MAEKLRVAFIDNSQAIDDQAIDAGEAKMTADKESLKGFSGFLKK
                     IWKHNLARNYYRQKEINTARQQILESGNLFAAAEGSQDEFIAEQEAIVDRFMADHAEM
                     IHQEAGESRRVIGETPEDKIFSDSLRNLIDKFVAGEINETELEEEKVRLLNSLPDFDD
                     KNGRLKVGCADNLLAIAKQAKQSLNHQSGLAKLDYDLEIIVGRAVSGVRTEAKYNKVD
                     RILEKLGKTKIGALINESTLGLAVAAATATAVSLGQTAGQMAARALGGLGLGALLTGG
                     LAAMRESQELKRERKQHSREMAQGKRFKKDDVRRQEMEKFNYKTIKSVDILSGLEINF
                     NEADTDNQAEIKQVVTNLADLEARIRLSDQQKIDLIAFSNLTNVEQERLELDLARARL
                     KVTLRRLADHDKLNFVDGNLDDYLNSLIEAKKQELLGGDNGIAEKDRLFNKMRKARMV
                     STGINAAGIGFAVGLAFQEAASFFRSDQQGLIEGAIKGRQPTSGHQSVTLLEKARAMI
                     FGQPKLQQAQELVRIGDHQFKVPVGTEIRPEADGEFSIFSGGKKLIGDLRLTNGQLSA
                     ESQADLAKHGINWLTQENRVAGSLGAQEVIEQNKKNFTNIVRRLWYDNDTKSFDKNEL
                     RVFWGGKYGSGLDQDGNILLNMQKMAPDGSYHTLAGQKLSADAQQLIKNGGLKLLLSL
                     SKGTQNQVIEIPINSDGLAVIPKGSLAASLFSHANGVTKFTGQFMEVAQTMGSVENGA
                     EAVRILATQVGQGMPDGSTIINEIVHSIDIPAVTTWEVPPIIPIMGRRPLEPAIVEQF
                     IKYNGYHSQELSKEEKTSYEKYFSPRLKNDPQAKLEQQQELDWFFEQKQTREPEHFNN
                     INQVVEADQLLSNLPKDTKLMVCMAVGAAYESENIYRTLKLYSKQDKAVLAKTVFVLN
                     VNWFDDDKNDPDKLQKIQKTIAEINKIKSEFPQLQIAAFNQEYIREVMIKERGANIHG
                     QFIKDVHDVALRSIQLSGINGDIFMLSNDADCQGMSDNYLAKMLD"
     gene            complement(3072..3377)
                     /locus_tag="PKV49_03325"
     CDS             complement(3072..3377)
                     /locus_tag="PKV49_03325"
                     /inference="COORDINATES: protein motif:HMM:NF039821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HPL22669.1"
                     /translation="MSTLSPSLDKFVDELLVAQGIDQLEPEIRDQLVQDLTDRLNDRL
                     EAAIVAELPEEKLPELEVIIDKKAGNEELQKFFAQNIPDLPAILARELAAFRQSYLG"
     gene            complement(3452..4306)
                     /locus_tag="PKV49_03330"
     CDS             complement(3452..4306)
                     /locus_tag="PKV49_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22670.1"
                     /translation="MKIIRILSILVLAILSLGNLVIFFVLSERAQATLTKGSTLALMV
                     LLAIILFFCEKRLAVMAESFFRANYPIAWKDYGNIWPVIVALLWADMSIVILYFLCAL
                     SASLGLFIAPLVGLISFYELERKLNRRRPCPVVNQKFWLAIDENSSLASMASQYSPQY
                     WGYGWFRHKKTTLPSREVRQFVLIEVSGEKLEEVITEISASGLMIPEAEWAEAFLTTF
                     LPKKYGVIAVADPVFIDWDGGKCYPLIKKDQLIFCSIADLNQHFIYDSTQIYHQKVWL
                     VEIPNNGR"
     gene            complement(4437..4555)
                     /gene="rrf"
                     /locus_tag="PKV49_03335"
     rRNA            complement(4437..4555)
                     /gene="rrf"
                     /locus_tag="PKV49_03335"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1348 a         1044 c          758 g         1422 t
ORIGIN      
        1 catctagcat ttttgccaaa taattatccg acattccttg acaatcagca tcattagata
       61 acataaaaat gtcaccatta ataccagaca gctgtatgga acgcagagcc acatcgtgaa
      121 catccttaat aaattgacca tgaatattag ctcctcgctc cttaatcatt acttctcgaa
      181 tatattcctg gttaaacgcg gcaatttgca attgcggaaa ttcacttttt attttattaa
      241 tctcagcaat ggttttttgg attttttgta atttatccgg atcattttta tcgtcatcaa
      301 accaattaac attcaaaaca aaaactgttt tggctagaac tgccttatct tgtttagaat
      361 ataattttaa tgttctataa atattctctg attcatacgc agctccaaca gccatacaaa
      421 ccattaattt agtatctttt ggtaaattgc ttaataactg atcggcttca accacttgat
      481 taatattatt aaaatgttct ggttctctgg tttgtttctg ctcaaaaaac caatctaact
      541 cttgctgttg ttctaatttg gcttgtggat cattctttaa tctaggagaa aaatacttct
      601 catagctagt tttttcttcc ttagacaatt cttgactatg atagccatta tatttaataa
      661 attgctcaac tattgccggt tctagcggcc gccgacccat aatcggaata atcggcggaa
      721 cttcccaagt tgtaacggcc ggaatatcaa tactatgaac tatttcgtta ataatagtgg
      781 agccatcagg cataccttgg ccaacttgag tggctaaaat tcgtaccgcc tcagcaccat
      841 tctcaactga tcccatagtt tgagccactt ccataaattg accggtaaat ttagtcactc
      901 cgttggcatg gctaaatagg ctggccgcca gactgccctt gggaataacc gctaagccat
      961 cagaattgat cggaatctca ataacctgat tttgcgtacc cttggataaa gataataaca
     1021 atttaagtcc gccattttta attagctgct gggcatccgc ggacaatttt tgaccagcca
     1081 atgtatgata cgaaccatca ggagccatct tttgcatatt taataaaata ttgccatctt
     1141 ggtctaatcc actaccatat ttaccgcccc aaaaaacccg caactcattc ttatcaaaag
     1201 atttagtgtc attatcatac catagccgtc gaacaatatt ggtaaaattt ttcttgtttt
     1261 gttcaatcac ttcttgagcg cccagcgatc ccgccactcg attttcctgg gttagccaat
     1321 tgataccgtg tttggccaaa tcagcttgtg attcggctga taattgtcca ttagttaacc
     1381 gcaaatcgcc aatcaatttt ttgccaccgc taaaaatact aaattcgcca tcagcctctg
     1441 gccgaatttc agtgccgacc ggcactttga attgatgatc accaatccta actagctctt
     1501 gggcttgttg cagtttcggc tgaccaaaaa tcattgccct agctttttct agcaatgtta
     1561 cgctttgatg accgctagtc ggctgccgtc cttttatagc tccttcaatc aaaccttgtt
     1621 gatcggaacg gaaaaaacta gccgcctctt ggaaagctaa gccaacagca aagccaatac
     1681 cggccgcatt aattccagta gagaccatcc tcgctttacg cattttatta aataaacgat
     1741 ctttttctgc aataccatta tctccgccta acaactcttg ttttttggcc tcaatcaaac
     1801 tgttaagata atcatcaaga ttaccatcaa caaaattaag tttatcgtga tcggccaaac
     1861 gtcttaaagt aaccttaagc cgagctctgg ccaaatcaag ctctaaacgc tcttgctcaa
     1921 cattagtcaa attagaaaaa gcaattaaat ctatcttttg ttgatcggat aaacgaattc
     1981 gagcctctaa gtcagctaaa ttagttacaa cctgtttaat ttcagcctga ttatcggtat
     2041 cagcttcatt aaaattaatt tccaagccgg ataaaatatc caccgattta atggtcttgt
     2101 aattaaattt ctccatttct tggcgtcgaa catcatcttt cttaaatctt ttaccctggg
     2161 ccatctcccg actatgctgc tttctttctc gttttaattc ttgagattcg cgcatggctg
     2221 ctaaaccacc ggttaataaa gcacctaaac ctaggccacc caaagctcta gccgccattt
     2281 ggccagctgt ttggcctaaa ctaaccgctg tggctgtagc cgcggccaca gccaatccta
     2341 gagttgattc attaattaac gctccaattt tagtcttgcc cagtttttct aaaatacgat
     2401 caactttatt atatttagct tcagtacgca ctccactaac agctcgacca acaataatct
     2461 ctaaatcata atctaatttg gctagaccgg attgatggtt taaagattgt ttagcttgtt
     2521 tggcgatggc taatagatta tcggcgcaac caaccttaag tcgaccattt ttatcatcaa
     2581 aatcgggcaa actatttaat aatctaactt tttcttcttc cagctcagtt tcattaattt
     2641 ccccagccac aaacttatca atcaaattac gcagtgagtc gctaaaaatt ttatcttcag
     2701 gcgtttcacc aataacgcgc cgactttcac cggcctcttg gtgaatcatt tcggcatgat
     2761 cagccataaa acgatcaacg atagcctctt gttcagcgat aaattcatct tgactacctt
     2821 cagctgcggc aaaaagatta ccagattcca aaatttgttg tctggctgta ttaatttctt
     2881 tctgacgata ataatttctc gccagattat gtttccaaat ttttttcaaa aaaccactaa
     2941 aacccttaag actctccttg tcagccgtca ttttagcctc gccagcgtca attgcttgat
     3001 catcaatcgc ttgagaattg tcaataaagg ctactcttaa tttctcggcc atataattac
     3061 aactagttaa cttaacccaa ataagattgc cggaaagctg ccaattctct ggctaaaatt
     3121 gccggtaaat caggaatatt ttgagcaaaa aatttttgca attcttcatt tccagccttt
     3181 ttatctataa taacttctaa ctctggcaat ttttcttccg gtaactcggc aactatggcc
     3241 gcctctaggc gatcgttaag cctgtcggtt aaatcttgaa ccaattgatc ccgaatctct
     3301 ggttctagtt gatcaattcc ttgagctacc aataattcat cgacaaattt atccaatgat
     3361 ggtgagagag ttgacataag acaaatttaa aaaataaact aatttaagtc tagcgataaa
     3421 ataataaaaa atcaaataaa aaggccgttg attaacggcc gttgttagga atttctacca
     3481 accagacttt ctggtggtag atttgggtac tatcataaat aaaatgctga ttaagatcag
     3541 cgatactgca aaatatcagc tgatcttttt taatcagggg gtaacactta cccccgtccc
     3601 agtcaataaa gaccgggtcg gctacggcaa tcacgccgta ttttttgggc aagaaggttg
     3661 ttaaaaacgc ttccgcccac tcggcctcgg gtatcattag gccagaggcg ctaatctcgg
     3721 taatcacctc ttccagtttt tcacccgaaa cctctattaa cacaaattga cgcacctccc
     3781 gacttgggag ggtggttttt ttgtgtctaa accagccata gccccaatat tggggtgagt
     3841 actggctagc catgctagcc aggctactgt tttcatcaat ggctaaccaa aatttttggt
     3901 taaccactgg acagggtcgc cggcggttta actttctttc taattcatag aaagaaatta
     3961 gcccaaccag aggggctatg aagagaccca aagatgccga taatgcacac aagaaataca
     4021 agattactat ggacatgtca gcccataata gggcaacgat aaccggccaa atattaccgt
     4081 agtctttcca ggctatggga tagttggctc tgaaaaaaga ttcagccatg acggctaatc
     4141 ttttttcaca gaaaaacaaa attatcgcta gcaataccat taatgctagc gtagatccct
     4201 tggtcaatgt cgcttgagct ctctccgaga gcacaaagaa tatgaccaga ttgccaagag
     4261 acaaaatggc caataccagg atcgacaaaa ttctgataat tttcatgatc tactcctctt
     4321 ctttttttta ttttttaatg ttcttttgtc aatcaaaatc tagattgact aattagtcta
     4381 caacaattct ggctatatat caatacttgc aacgatcgaa taaaaaaaca ccataattct
     4441 ggcgctgacc tactttccca gggcttgatg cgacccaagt atcatcagta ctggaaggct
     4501 taactactga gttcgggatg ggatcaggtg gaacccttcc gtttgaagca ccagaattat
     4561 ggtgtctttt at
//
LOCUS       DAOPAI010000035         4181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_17441_length_4181_cov_4.734610, whole genome shotgun sequence.
ACCESSION   DAOPAI010000035 DAOPAI010000000
VERSION     DAOPAI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17441_length_4181_cov_4.734610"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..368
                     /locus_tag="PKV49_03340"
     CDS             66..368
                     /locus_tag="PKV49_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22671.1"
                     /translation="MKIHLSASRPTMTWPQLMRRAGYAYLPGRSGNDSFIRTFGRSPY
                     PRFHAYIKDQGRQAVINLHLDQKQPGYGGQARHNGEYDGPVVAAEAARLQQLFNHG"
     gene            361..2172
                     /gene="dnaG"
                     /locus_tag="PKV49_03345"
     CDS             361..2172
                     /gene="dnaG"
                     /locus_tag="PKV49_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPL22672.1"
                     /translation="MVNPSDEIKARLNVADVLGEYIKLQPAGGNFKAKCPFHQEKTAS
                     LMVSPDKQIWHCFGCGKGGDIFSFVMEMEGLTFPEALRLLAPKAGIQLTDYRGQDNSR
                     RNRLLDALAEAGVFYQKQLTANNKLAEAALHYLRIERHLTDDMLNYWQIGWAPNDWQM
                     LSDYLRRKGYTENELLLAGLNSQAATGQRFYDRFRGRIMFPWHDVNGNLLGFSARVLP
                     QLDDGKTGKYINSPQNLIYDKSKQLFGLWRAKAAIKEAGRVIIVEGQMDCIMAHQQGF
                     KNVVATSGTALTEDHLKLIKRYTENLVLAFDMDQAGILAADRAIALASQVNMNIRVLR
                     LPAGKDPDEFLRQHNQDWPVLVEAAPPIMEYYFQLLRPQLVADKVESRKAATKRFLTL
                     ISKLSDVVERDYWLKKLAQVLRIEVQLLYDSLPTNQPTTNQPTTNQPIKQLVKNRPVD
                     LFLAWLIRFPSLLDNASQWLEPELLNSPTAVQIYKNLIVYYNKKRLTAGDNLDYNDLL
                     AWWPADQSGDYLNSLFLLADKDLAETTIEQALIELPKLTRLIQRAYWHQRLSVISQQI
                     AGLEKDKSLSDDKRELEINILMGEAEELSQALRTLDN"
     gene            2370..3443
                     /locus_tag="PKV49_03350"
     CDS             2370..3443
                     /locus_tag="PKV49_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPL22673.1"
                     /translation="MIDRVRPRGFVTETELLYLLPKLEDYVYDCELILEELQRNSIKV
                     VETGEGYLETGAEKVKKEELMRSVLGEIKDIKKFDISELSADSVQMYLKEIGKVPLLT
                     TAEEVELARRKERGDREAERRLIEANLRLVVSIAKKFSGTRGLSLLDLIQEGNIGLFR
                     AVEKFEYRKGYKFSTYATWWIRQAITRSLADQSRTIRIPVHMVETINKFQQVQRQLIQ
                     DLGREPMPEDIAAEMGEDLDKIRHVMKISQDTISLETSVGDDDDDSTLEDFIEDVKNM
                     TPDRVAALELLKDYVKEVIATLTPREQKILEMRFGLADGVSHTLEEVGQEFEVTRERI
                     RQIEAKALEKIKKQEAVKKLKDF"
     gene            3453..3758
                     /locus_tag="PKV49_03355"
     CDS             3453..3758
                     /locus_tag="PKV49_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22674.1"
                     /translation="MDRELPDLKKEPDKIGYVISFIFGGTLFGIMVQDGFSPTLCALA
                     AAAFMIFGFVAQSLCNRYGDIKISKLNSGSLFFILVGLMFVAMFLALIFDVGGTDGI"
BASE COUNT         1185 a          768 c         1018 g         1210 t
ORIGIN      
        1 ttaatggata aaacataagc ttccggagct tgcgatgggg gttcgattcc ctctgggggc
       61 acactatgaa aattcatctc agcgctagcc gtccaaccat gacttggccg caattaatgc
      121 ggcgagctgg ttatgcttat ctgcccggac gatccggtaa tgatagcttt attcggactt
      181 tcggccgctc gccttatccg cgttttcatg cctacattaa agatcaagga cggcaagcgg
      241 tgattaattt gcatttggat caaaaacagc ccggctatgg tggccaagcc cgacataatg
      301 gcgagtatga cggtccggtc gtcgcggctg aagcggcgcg tttacaacaa ttatttaacc
      361 atggttaatc ctagtgacga aatcaaagcc cgtctcaatg tggccgatgt tttgggcgaa
      421 tatattaaat tgcagccggc tggtggcaat tttaaagcta agtgtccgtt tcaccaagaa
      481 aaaacggctt cgttgatggt ttcacccgat aagcagattt ggcactgttt cggttgtggc
      541 aagggcggcg atatttttag ttttgtcatg gaaatggaag gcttaacttt tccagaagct
      601 ctgcgcttat tagcgccaaa ggcggggata caattaactg actatcgggg gcaggataat
      661 tcacggcgca atcgattatt ggatgctttg gctgaggctg gagtttttta tcaaaaacag
      721 ttgactgcta ataataaact agctgaagcg gcgctgcatt atttacggat tgaacggcat
      781 ctaactgatg acatgcttaa ttattggcaa attggctggg cccccaatga ttggcaaatg
      841 cttagtgatt atcttcggcg gaaaggctat acggaaaatg aattattact ggctggactt
      901 aatagccaag cggcgactgg tcagcgtttt tatgatcgct ttcgcggccg aatcatgttt
      961 ccttggcatg atgttaatgg taacctgctg ggtttctcgg cccgtgtttt gccgcagtta
     1021 gatgatggta agactggtaa gtatattaat agtccacaga atttaattta tgataaaagc
     1081 aaacaattat ttggtttgtg gcgggctaaa gcagctatca aagaagctgg ccgagttatt
     1141 attgttgagg gacagatgga ttgtattatg gctcatcagc aaggttttaa gaatgttgtg
     1201 gccacttccg gcacggcctt gactgaagat catttgaaat taatcaagcg ttataccgag
     1261 aatttagttt tagcttttga tatggatcag gctggtattt tagcggccga tcgagcgatt
     1321 gctttagcta gtcaggttaa tatgaatatt cgggttttgc gcttgccggc cggtaaagat
     1381 cctgatgaat ttttacgcca acacaatcaa gactggccag ttttggtcga agccgctccg
     1441 ccaatcatgg agtattactt tcaattatta cggccgcaac tcgtagccga caaggtggaa
     1501 agccgcaagg cggccactaa acgtttttta actctaatca gtaaattatc agatgtcgtg
     1561 gagcgtgatt attggttgaa aaaattggct caggttttgc gtattgaagt acagctgctt
     1621 tatgatagtt tgccaaccaa ccagccgacg actaatcagc caacaaccaa ccagccaatt
     1681 aagcaattag taaaaaatag accagttgac ttatttttgg cttggctgat taggtttcct
     1741 agccttttgg ataatgccag tcagtggtta gaaccagaat tattaaactc gccaaccgct
     1801 gtgcagattt acaaaaattt gattgtttat tataataaaa agcgtttaac ggccggtgat
     1861 aatttggatt ataatgattt gttagcttgg tggccagccg atcagtcagg ggactatttg
     1921 aatagtctat ttttgttagc tgataaggac ttagctgaga cgaccattga acaagcttta
     1981 attgaattac ctaaattgac taggttaatt caaagagcat attggcatca gcgtttgtca
     2041 gtgattagcc aacagattgc cggactagaa aaagacaaat cattgagcga cgataagcgt
     2101 gaattggaaa tcaatatttt aatgggggag gctgaagaat tgagccaagc cctgagaact
     2161 cttgataatt aaattttttc atgactaaaa agaaattagc taattcggtt agcctcggcg
     2221 agattatcca aaagcagacg gccgatttta cgccgtcaac tttgccgacc gataaaatta
     2281 aaaaatcagc caaagcgacg ccgcgagctg acaagcattt caaaaaagat aaaccggcgt
     2341 caggaccgac cgaacagcag attaaggcaa tgattgaccg agtgcggccg cgcggttttg
     2401 tgactgaaac agagctgctt tatttattgc ccaagctgga agattatgtt tatgattgtg
     2461 agctaatttt agaggagttg caacgtaaca gtattaaggt tgttgagacc ggtgaggggt
     2521 atttagaaac cggtgctgag aaggttaaaa aagaagagct gatgcgatca gttttaggcg
     2581 aaattaaaga tattaaaaag tttgatattt ccgaattgtc cgccgattca gttcagatgt
     2641 atctgaaaga aatcggtaaa gtgccattat taaccaccgc tgaagaagtg gagttggcgc
     2701 ggcgcaaaga acgtggcgac cgcgaagccg agcgtcgtct cattgaagcc aatttgcgtt
     2761 tagttgtttc tattgctaaa aagtttagcg gtacacgggg gttaagttta ttagatttga
     2821 ttcaggaagg caatatcggt ttatttagag cagttgaaaa atttgaatac cgtaaggggt
     2881 ataagttttc cacttatgcc acttggtgga ttcgccaagc gattactcgt tcattggccg
     2941 atcaatcgcg gactattcgc atcccagtac atatggtgga aacaatcaat aagttccagc
     3001 aagtccagcg tcagttgatt caagatcttg gtcgcgagcc gatgcccgaa gatatcgccg
     3061 ccgaaatggg tgaagattta gataagattc gccatgtcat gaagatttct caagatacta
     3121 tttctctgga aacatcagta ggtgacgatg atgacgattc taccctggag gacttcattg
     3181 aagatgttaa aaatatgact ccggaccgag tagccgctct agagttgctt aaagattatg
     3241 ttaaagaagt gattgccact ttaaccccga gagagcaaaa gattttagaa atgcgttttg
     3301 gtttggccga cggtgtttcg catactcttg aggaagttgg tcaagaattt gaagtaaccc
     3361 gcgagcgcat ccgtcagatt gaagccaaag ccttagagaa gattaaaaaa caagaagcag
     3421 tcaagaaatt aaaggatttc taaaaatttg ccatggatag agaattacca gatttgaaga
     3481 aagagccaga taagatcggt tatgtcatta gctttatttt cggcggtaca ctttttggca
     3541 taatggtcca agatgggttt agtccaacgc tatgtgcttt ggcagccgca gcttttatga
     3601 tttttggctt tgtggcgcaa agtctttgta atcgttatgg tgatattaag atatctaaac
     3661 tcaattccgg ttctttattc tttatattag tgggtttgat gtttgttgct atgtttttgg
     3721 ccctaatttt tgatgttggc ggcacagatg gtatttagta attgctattt ttattgacaa
     3781 gctaagagtc tttgctataa tagctctgta tttaacaatt aaatattccc gggtagcgca
     3841 gcggtagcgc agttggctgt gagctttaaa acgcagcttc ctcaagtaat tgaggttgaa
     3901 aaacgaggtg aattgtctgg aagcctaagc tcttaagaac taggagtagg gtaatcagca
     3961 gccaagccct ttttagggaa ggttcagaga ctatccatta tggagtactt cttcagctaa
     4021 aactgaagcg ggaagcgcct cgcatccttt actggatgat gatatagtcc accccattaa
     4081 gaaattagtg gataagtgta accaattggt cgtcggttcg aatccgaccc cgggagccag
     4141 cgtgtacgaa cagaggttga agcctctgtt tgtgttttat t
//
LOCUS       DAOPAI010000036         3881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_19058_length_3881_cov_4.666492, whole genome shotgun sequence.
ACCESSION   DAOPAI010000036 DAOPAI010000000
VERSION     DAOPAI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19058_length_3881_cov_4.666492"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /gene="galE"
                     /locus_tag="PKV49_03360"
     CDS             <1..782
                     /gene="galE"
                     /locus_tag="PKV49_03360"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPL22675.1"
                     /translation="GVLHFAALCLVDESMSQPERYFYNNVVGSLNLLEQLRQHGCPPL
                     IFSSTCAVYGESQYLPIDEQHPTNPTNPYGESKLIVEKMIQWYGSQHQLPYVILRYFN
                     VCGADSQGLIGDSKKPSQLLMQNAVRGALNIEPFAYTCGQVATPDGTPIRDYIDVEDL
                     VAAHAAAYDYLVAAGSSQIINLGNGQGYSVKEIVSAVETNLHCQLVKAQAPTRLGEYA
                     NVYANNSKAKQLLAWQPNKSLDDSIASLTRWYKKFPNGYQF"
     gene            921..1160
                     /gene="infA"
                     /locus_tag="PKV49_03365"
     CDS             921..1160
                     /gene="infA"
                     /locus_tag="PKV49_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPL22676.1"
                     /translation="MTKAVNETNASKNFLECEGEILELMPGLTFKVQLENGHELLAHL
                     SGKMRINKIRLAPGDRVRLEISPYDLTKGRVIYRL"
     gene            1177..1290
                     /gene="rpmJ"
                     /locus_tag="PKV49_03370"
     CDS             1177..1290
                     /gene="rpmJ"
                     /locus_tag="PKV49_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPL22677.1"
                     /translation="MKVRASVKKICKDCKIIRRFGRVQVICKNPKHKQRQG"
     gene            1309..1701
                     /gene="rpsM"
                     /locus_tag="PKV49_03375"
     CDS             1309..1701
                     /gene="rpsM"
                     /locus_tag="PKV49_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPL22678.1"
                     /translation="MVVRIAGVTLPNDKRIEVALTYIYGIGPTKARKVLTAAKLSPDI
                     RTKDLSEDQANQLRILIEKNERVEGDLRREVGGNIKRLKDINTYRGLRHSRNLPCNGQ
                     RTKTNSRTVRGNRRQTMGSGRKPTGQKT"
     gene            1891..2283
                     /gene="rpsK"
                     /locus_tag="PKV49_03380"
     CDS             1891..2283
                     /gene="rpsK"
                     /locus_tag="PKV49_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPL22679.1"
                     /translation="MAATKSKRRRRKTERTLTVGSAYIKSTYNNTIVTITDENGDVVS
                     WASAGLAGFKGPKKSTPYAAQIITRIAVERAKEMGLTDVSVFVNGVGTGREAAVRALN
                     ANGLVVTSIKDVTPVPHNGCRQRKPRRV"
     gene            2304..2939
                     /gene="rpsD"
                     /locus_tag="PKV49_03385"
     CDS             2304..2939
                     /gene="rpsD"
                     /locus_tag="PKV49_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPL22680.1"
                     /translation="MSKYLGSACKLCRREGEKLMLKGERCSSSKCALAKRNFPPGFHG
                     PKGRPRQSEYSLQLREKQKAKRMFGLLEKQFHLTFLAAKKQVGDAGENLMRLLEMRLD
                     NVVYRAGFADSRSKARQLVAHAHFTVNGRRVDIPSYRVKSGDIIKVKASQQRNKIFNG
                     LGEKLLKKNFQAPGWLFLEVADLEVKVLHEPKPEDLEKNINTQMIVEFYAR"
     gene            2968..3831
                     /gene="rpoA"
                     /locus_tag="PKV49_03390"
     CDS             2968..3831
                     /gene="rpoA"
                     /locus_tag="PKV49_03390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPL22681.1"
                     /translation="MEKIALPQKITVLPGNQPNEELIVVEPFFPGYGTTIGNALRRVL
                     LSSLPGAAVVGVKIAGADHEFMGLPGIQEDVLAIVLNLKKLRLRIFSDEICKLTLEVS
                     GQKVVTAADITPNSQVEIVNPDLVLANLTTKEANLSMEIFVSNGRGYEMVENRLKTKK
                     EIGYIEIDSMYTPVMAAGLTVENVRVGNMVNWDKLILRILTDGTTSPHQAFVDANDIL
                     ISQFSALVNLPEEDNADTAQVADQPAGETEDIVETDDVQEEVKKPSKKPAAKKEVAAK
                     PAKKPTKAKTK"
BASE COUNT         1103 a          737 c          918 g         1123 t
ORIGIN      
        1 ccggcgtgct ccattttgcc gctctgtgtt tggttgatga atcaatgtct caaccggagc
       61 gttattttta taataatgtg gttggcagct tgaatctgct ggagcaactg cgtcaacatg
      121 gttgtccccc gcttatattt tcgtcgactt gtgccgttta tggggaaagt caatatttac
      181 caattgacga acaacatcca actaatccaa ccaatcctta tggggaatct aaattgatag
      241 tggaaaaaat gattcagtgg tatggttcgc aacaccagct gccctatgtt attctgcgtt
      301 attttaatgt ttgtggagcg gatagccagg gtttaatcgg cgatagcaag aaacctagcc
      361 agttattgat gcagaatgcg gtgcgtggcg ctttgaatat tgagccattt gcttatactt
      421 gcggtcaagt agccacaccg gatggcacac cgatccgtga ttatattgac gtggaagact
      481 tggtggctgc tcatgccgct gcttatgact atttagtggc ggccggttct agtcagatta
      541 ttaatttagg caatggccaa ggctatagcg ttaaagagat tgttagtgcc gtcgaaacta
      601 atcttcactg ccaactagtt aaagctcagg caccaactcg tctcggtgag tatgctaatg
      661 tttatgctaa taattccaag gccaagcaat tactagcttg gcagcccaat aaaagtttag
      721 atgatagtat tgctagctta acccgctggt ataaaaaatt tcctaatggt tatcagtttt
      781 agattagcta agagtacatc ttgacagatg tcgactttgc ctttataatt agaccatatt
      841 tacaaattaa atagtcagac atagcgataa tttatcagtt tttggcttga taaattattt
      901 tatgttagta aaaataatta atgaccaaag ctgttaatga aaccaacgcg tccaaaaatt
      961 tcctagagtg tgaaggggaa attttagaat tgatgcctgg tttaaccttc aaggttcagt
     1021 tagaaaatgg ccacgaatta ttagcccact tgtcaggcaa gatgcggatt aacaagattc
     1081 gtttagcgcc aggcgatcgg gtgcgcctag agatcagtcc ttatgatttg actaaggggc
     1141 gggtcattta ccgtctctaa ttaatgtaga gcaagtatga aagtgagagc ttcagttaaa
     1201 aaaatctgta aagattgtaa aattatccgc cgatttggtc gggtgcaagt gatttgtaaa
     1261 aatcccaagc acaagcaacg gcagggttag ttttaatttt atatttatat ggtagtacgt
     1321 atagcgggcg ttaccctgcc caatgataaa cggattgaag tcgctttgac ttatatttat
     1381 ggcatcggtc cgactaaggc gcggaaggtt ttaactgccg ccaaattaag tcccgatatc
     1441 cgcactaaag atttaagcga agatcaggct aatcaattac gtattttgat tgaaaaaaat
     1501 gaacgggtgg aaggtgattt gcgtcgcgaa gtcggcggca atattaagcg cttaaaggat
     1561 attaatacct atcgcggttt gcgccatagt cgcaatttgc cgtgcaatgg ccagagaacc
     1621 aagactaata gtcggactgt ccgcggcaat cgccgccaga ccatgggttc cggccgtaag
     1681 ccgaccggtc agaagaccta gtttcaagta attaatgaag ttgtttttat gactgacgaa
     1741 caaaaaaagg tggccgaaga gattgtgact gcaacaacgg cggctccgac aaccactgct
     1801 gatcagccag cgccagccgc taccggtccg gtaatggaag agttgcctga tgaaatcaag
     1861 aaaaaattgg aaaagaataa agccaatcgt ttggctgcta ctaagagcaa gcgtcgccgc
     1921 cgcaaaactg agcggacttt gactgtcggt tcggcttata ttaagtccac ctataataat
     1981 actattgtta ctattaccga tgaaaatggt gatgttgttt cttgggctag tgccggttta
     2041 gccggtttta agggcccaaa aaagtctact ccatacgctg ctcagattat taccagaatt
     2101 gctgttgagc gagccaagga aatgggtttg accgatgtta gcgtttttgt taacggtgtc
     2161 ggtactggtc gtgaagcagc ggtccgagcg ctcaatgcca atggtttagt agtgacttct
     2221 attaaagatg tgacgccagt acctcacaat ggctgccgtc aacgcaaacc ccgtcgggtc
     2281 taattattgt ttgtaatctc cgtatgtcca aatacttagg ttcagcttgt aaattgtgcc
     2341 gtcgtgaagg cgaaaagtta atgctcaagg gtgagcgttg cagttccagc aaatgcgccc
     2401 tagctaagcg taattttccg cctggttttc atggtcccaa gggtcgtccg cggcaatcgg
     2461 aatacagctt gcaattgcgc gaaaagcaaa aagccaagcg gatgttcggc ttgctggaaa
     2521 aacaattcca tttaactttc ttagccgcca agaaacaggt tggcgatgct ggtgaaaatt
     2581 taatgcgtct tttagaaatg cgtttggata atgttgttta tcgggccggt tttgctgatt
     2641 ctcgctccaa ggctcgccag ttagtggctc atgctcattt taccgttaat ggtcgtcgag
     2701 tggatattcc atcttaccgc gttaagtctg gtgatattat taaagttaaa gctagccagc
     2761 agcgtaataa gatttttaac ggattgggcg agaaattatt aaagaaaaat tttcaagccc
     2821 caggctggtt atttttagaa gttgctgatt tagaggttaa ggttttacat gaacctaagc
     2881 cagaagattt ggaaaaaaat attaatactc agatgattgt tgagttctac gctcgttaat
     2941 ttttagctca aatttaaaac atccattatg gaaaaaattg ctttaccaca aaaaataact
     3001 gttttgccag gcaaccagcc caatgaagag ttgattgttg ttgagccgtt ctttccaggc
     3061 tatggcacaa ctatcggtaa tgctttgcga cgagtgctgt tgtcttcttt gccgggtgca
     3121 gctgtggtcg gggtcaagat tgctggcgct gatcatgaat ttatgggtct acctggtatc
     3181 caagaggatg ttttagctat agttttgaat ttgaaaaaat tgcgattacg gatttttagc
     3241 gatgagattt gtaagctcac tctagaagtt agcggtcaga aagtggttac cgctgccgac
     3301 attactccta atagtcaagt ggaaatcgtc aatccagatt tggttttggc caatctgacc
     3361 actaaggaag ccaatttgtc aatggagatt tttgttagca atggtcgtgg ttatgaaatg
     3421 gtggagaatc gtctcaaaac taagaaagaa atcggctata ttgagattga ttccatgtat
     3481 acgccagtta tggccgccgg tttaactgtg gaaaatgttc gtgtcggcaa tatggttaat
     3541 tgggataagt tgattttgcg tattttaacc gatggcacta cctcgccaca tcaggccttt
     3601 gtcgatgcta atgatatttt aattagtcag ttctctgctt tagttaattt gcctgaagag
     3661 gacaatgcgg acactgctca agttgctgat cagccggctg gagagacgga ggatatagtt
     3721 gagaccgacg atgtccaaga agaagtcaag aaacctagta agaaaccggc cgccaagaaa
     3781 gaagttgccg ctaagccagc caaaaagcca accaaggcta aaactaaata atttttacat
     3841 taaagagttt taaggatatt gtatgcgtca ccgaaataaa g
//
LOCUS       DAOPAI010000037         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_19432_length_3819_cov_3.747875, whole genome shotgun sequence.
ACCESSION   DAOPAI010000037 DAOPAI010000000
VERSION     DAOPAI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19432_length_3819_cov_3.747875"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PKV49_03395"
     CDS             complement(<1..1520)
                     /locus_tag="PKV49_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPL22682.1"
                     /translation="MSQTLKIDNQTSQRRYFIDKSFLMPLPDLIEVQKKSYAWFLKEG
                     LADLFDEISPISDFMGRDLELYLTDYYFDEPKFDEAESKAKNVTYEAALRVKARLVNK
                     KTGQSISQEVFLGDFPLMTQNGTFIINGIERAVVSQLIRSAGVIFTAENIRGRKYYGA
                     KIIPNRGAWIEIETDVNHVLWARIDRKRKVAVTSLLRAFGYGEDEDIKKALSGVDIAD
                     GVSYIDATIDKDLAKNEAEGLKEVYKRIRPGDLATSENAKQLIHSMFFRFDRYDFDRV
                     GRYKLNQRFNFDIPNNKETRILRPEDLVAIIKEVIRLNITQEPEDDIDHLGNRRVRAI
                     GELIQNKTRVGLSRMERIVRDRMSILDIGEITPNKLINARPVISVVKEFFMSSQLSQF
                     MDQTNPLAELEHKRRLSALGPGGLARERAGFEVRDVHATHYSRICPIATPEGPNIGLV
                     GHLASYARINDFGFIEAPYCRVIHDIENEPAITVGQTIRQDIEWLDQKTKKTTIIA"
     gene            complement(1776..3194)
                     /gene="murE"
                     /locus_tag="PKV49_03400"
     CDS             complement(1776..3194)
                     /gene="murE"
                     /locus_tag="PKV49_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HPL22683.1"
                     /translation="MNKLLARCKRFIYHSFFRRLIPSYHFILNWLAAAYYHHPSQQLI
                     VIGVTGTTGKTTSVFLIAEVLRAAGYKVGYTSTAMFSDGQKDWLNDKKMTMVGRFFTQ
                     RLLRQMVNNGCHYAIIETTSQGILQYRHRFINYDIAVFTGLYPEHIEAHGGFDNYRRA
                     KGRLFSHLRAGHYKYINDQQRVQSTDRGFKRIGLNKVLKTVIVNLDDEYASYFLSFGF
                     DQAFGYTQKPKEIEEHNWPEQLKILPIEQLQAGIHGTDFVCQDQMIHLPILGAFNALN
                     ALNAIAVALSQEIDWSIIRSGLAAIPGLAGRLEMIDQGQPFSVIVDYAFEPVALSKIY
                     ETLELVNYKKIIHVLGSAGGGRDVARRPKLGQIAGQLADYVIVTNEDPYDDDPRLIID
                     QVAVGAEHAGKILNKNLLTILDRREAIAKALSLAKKDDIVLITGKGSEQAICLAAGRK
                     LPWDDRQVVREELAKLAIDGWQ"
     gene            complement(3207..>3819)
                     /locus_tag="PKV49_03405"
     CDS             complement(3207..>3819)
                     /locus_tag="PKV49_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPL22684.1"
                     /translation="LGLIHGPDIQPSQTRILDISQTEEALLQQMHPKTRYNIRLATKR
                     GVVIEAVGPDGFTDFWRLMAITGQRNRFHLHQPQYYRAIIKAPFSQLLVAKWQGQILA
                     AGIFTYWGRRAVYLHGASDNKQRQLMAPYLIQWQAILTARQRGCQTYDFFGINQQKWP
                     GVTRFKQGFGGKIVNYPGTFDWPINQYFYRFYQLIRQLRRLLR"
BASE COUNT         1211 a          839 c          718 g         1051 t
ORIGIN      
        1 ttagcaataa tagttgtctt cttggttttt tggtctaacc attcaatatc ttgacgaata
       61 gtttggccaa cagtaatggc cggttcgttt tctatatcat gaataacgcg gcaatagggt
      121 gcctcaataa aaccaaaatc attaattcgg gcatagctgg ctaaatggcc aaccaaaccg
      181 atattcggac cctctggagt ggcaatcgga caaatacggc tataatgagt agcatgaaca
      241 tctcggactt caaaaccggc ccgctcacga gctaaaccgc caggaccaag agctgataac
      301 cggcgcttat gctccaattc agctaatgga ttcgtctgat ccatgaactg cgataactgt
      361 gatgacataa aaaattcctt gaccacgcta ataaccggcc gagcattgat taatttattg
      421 ggagtaattt caccaatatc taaaatactc atacgatcac gaacgattct ctccatacgc
      481 gacaaaccaa cacgagtttt gttttggatt aattcaccga tggcgcgaac acgacgattg
      541 ccgagatgat caatatcgtc ttcaggttct tgagtaatgt ttaaccgaat aacttcttta
      601 ataatagcca ctaagtcttc cggccgtaag atacgagttt ctttattgtt aggaatatca
      661 aaattaaacc gctgattcag tttataacgg ccaacccggt caaaatcata acgatcaaag
      721 cggaaaaaca tggaatgaat caattgttta gcgttttcag aagtagctaa atcacctgga
      781 cgaatacgct tataaacttc cttaaggccc tcggcctcgt ttttggctaa atctttatca
      841 atagtcgcgt caatataact gacaccgtca gcaatatcca caccggacaa ggccttttta
      901 atatcttcat cctcgccata accaaaggct ctcaataaag aagtgaccgc cactttacgc
      961 ttacgatcaa tacgggccca taaaacatga ttgacatcag tctctatctc gatccaggcg
     1021 ccgcgattag gaataatttt agcgccataa tatttgcggc cgcgaatatt ttcagctgta
     1081 aaaataacgc cggcactacg aattaattga gaaacaacgg cccgctcaat gccgttaata
     1141 ataaaggtgc cgttctgagt cattaatgga aaatcaccca aaaaaacttc ttgagaaatt
     1201 gattgtccgg tttttttatt aactagccgg gccttgaccc gcaaggccgc ttcataggta
     1261 acattcttgg ctttactctc agcttcgtca aacttgggtt cgtcaaaata ataatcagtc
     1321 agataaagct ctaggtcacg acccataaaa tcgctaatcg gcgaaatttc gtcaaacaaa
     1381 tcagccaatc cctctttaag aaaccaggca taggattttt tttgcacctc aatcaaatca
     1441 ggcagcggca ttaaaaagct tttatcaata aaataacggc gctgactggt ttgattgtcg
     1501 attttaagcg tttgcgacat atttttgaca acaaaaaatt ctcaaagcct aatggcgaat
     1561 gagttagcta aaactatata attgtttaat tggagccgcg gataggggta tatcttatgg
     1621 caaaatcaaa gatagccgat aaaatgacca aaagctacgc tttgaccgcg tgctagtcat
     1681 ttagttagtg ttttctctga tctaagctta gaattgagat tgacaaattt gtctaataaa
     1741 gagtgagtca atctaatatt gttaagataa caaaattatt gccagccgtc aatagctaac
     1801 ttagctaatt cttctcgaac cacttgacga tcatcccatg gtaattttcg gccagccgct
     1861 aagcaaatag cttgctcgct acccttaccg gtaatcagga caatatcgtc ttttttggct
     1921 aaagacaagg ctttggcaat ggcttctcgg cgatccaaaa ttgttaataa attcttatta
     1981 agaatcttgc cagcatgctc agcgccaaca gctacctgat caatgattaa acgcggatca
     2041 tcatcataag gatcctcatt agtaacaata acataatcgg ccaattgtcc agcaatttga
     2101 ccaagcttag gtcgccgagc aacatctcgt ccaccgccag ctgaacctaa aacatgaatt
     2161 atttttttat aatttaccaa ttccaaggtt tcataaatct tgcttaaggc caccggttca
     2221 aaagcataat caacgatgac gctaaatggc tggccctgat caatcatttc gaggcgacca
     2281 gctaaccctg gaatagccgc taagccggaa cgaataattg accaatcaat ctcttgactt
     2341 aaagcgacag caatggcgtt aagagcattc aaggcattaa aggcgcccag tattggcaag
     2401 tggatcattt gatcctgaca aacaaaatca gtgccgtgaa tcccggcttg caactgttca
     2461 atcggtaaaa tttttaattg ttccggccaa ttatgctctt ctatttcttt aggtttttgg
     2521 gtataaccaa aagcttggtc aaaaccgaaa cttaaaaaat aggacgcata ttcatcgtca
     2581 agattaacga taactgtttt taaaacctta ttgagtccga tacgtttaaa accgcgatca
     2641 gttgattgaa ctcgctgctg atcattaata tatttatagt gaccagcgcg caaatggcta
     2701 aacaagcggc ctttagcccg acgatagttg tcaaaaccac catgagcttc aatgtgttca
     2761 ggataaagac cagtaaaaac agctatatca taattaataa agcggtgacg atattgtaaa
     2821 ataccttgcg atgttgtctc aataatagca taatgacaac cattattaac catttgtctt
     2881 aagagccgct gggtaaaaaa acgaccaacc atggtcatct tcttatcgtt aagccaatct
     2941 ttttgaccat cgctaaacat ggctgttgag gtataaccaa ccttataacc agcggctcga
     3001 agcacctcag caatcaaaaa gacgctagtt gtcttgccgg tagttccggt aaccccgata
     3061 acaattaatt gctggctggg atgatgataa taagcggcag ccagccaatt taaaataaaa
     3121 tgatagctag gaattaagcg acgaaaaaaa ctatgataaa taaatctttt acagcgagcc
     3181 aataatttat tcatagaatt attaatttag cgcaataaac gacgcaattg acgaattaat
     3241 tgataaaaac gatagaaata ttgattaatc ggccaatcaa aagttccggg gtaattgaca
     3301 atcttaccgc caaaaccttg cttaaagcga gtcacgcctg gccatttctg ttgatttata
     3361 ccaaaaaaat cataagtttg acagccgcgt tgacgcgccg tcaaaatagc ctgccattgt
     3421 attaaataag gagccattaa ctgccgttgt ttattgtcag aagcgccatg caggtaaacg
     3481 gctcgccgtc cccaataagt gaaaattccc gccgccagaa tctgaccctg ccacttggct
     3541 actagcaact ggctaaaagg cgccttaatt atggctcggt aatattgcgg ctggtgtaaa
     3601 tgaaacctgt tgcgttggcc agtaatggcc attaatcgcc aaaaatcggt aaaaccatct
     3661 ggtccgaccg cttcaatcac tactccccgc ttagtggcta aacggatatt ataacgcgtt
     3721 ttgggatgca tttgctgtaa aagagcttcc tctgtttgac taatatctag aatcctggtt
     3781 tggctaggct gaatatctgg cccgtgaatt aaaccaagc
//
LOCUS       DAOPAI010000038         3300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_23127_length_3300_cov_4.085362, whole genome shotgun sequence.
ACCESSION   DAOPAI010000038 DAOPAI010000000
VERSION     DAOPAI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23127_length_3300_cov_4.085362"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKV49_03410"
     CDS             <1..208
                     /locus_tag="PKV49_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22685.1"
                     /translation="LALDIQLLDGHGEVVELTDQTDDYDSRYNNNNNLDANAHINEVV
                     GEEMARRSSQALTEDAQSAGLEII"
     gene            230..>3300
                     /gene="rpoC"
                     /locus_tag="PKV49_03415"
     CDS             230..>3300
                     /gene="rpoC"
                     /locus_tag="PKV49_03415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPL22686.1"
                     /translation="MLNPIKTTDFEAIKLKLASPEEIIAWSHGEVTRPETINYRTQKP
                     EKDGLFCERIFGPSKDWECYCGKYKKVRYKGIVCDKCGVEVTRSSVRRERMGHISLQT
                     PVCHIWFLRGLSSKIGLALNISVQAIEKVIYFASFIITDVNQELLEATIEQIRQEYKS
                     KKKLFENEFAKGLAELEKQKQAALANGKTALEVKQMIDQSLEELTADRDAKISELEEA
                     LAQTQKELKDLRPMTILPENVYQNLSLKYGHIFTASIGAEGIRELLANIDLEKIIQEL
                     EAELATAIDSKKEKIVKRLRFFKNLLANNLRPEWMILKVIPVIPPDLRPMVALDGGRF
                     ATSDLNDLYRRIINRNNRLKQLIELKAPEVICRNEKRMLQEAVDALIDNSIRHGKTVT
                     ASTGQKRTLKSLSDSLKGKQGRFRQNLLGKRTDYSGRSVITVNPNLRLWQCGLPKRMA
                     LELFKPFIISELIKREVVHNVRSASRYIEADHDDVWEILENIVREAYVLLNRAPTLHR
                     LGIQAFKPILTEGLSIQIHPLVCSAFNADFDGDQMAVHVPLTEEARAEARDIMLASHN
                     LLKPATGDPIVNPGQDIVWGVYYITSEEKGAEQDVTKMKAFGSWQEAKLAYDCGKIRL
                     RETIRVQIDYLNNQLITTTVGRIVFNNILPEKLRFVNEIVLGPKVKALVKDCLRHYGE
                     EATVDFLDELKRESFKFITRSGLSWGMGDLPTFASRDELIKQAEQEAEEVQQQYEDGL
                     LTDSERYGKIIQLWTAAKDKITAICMEDLDRDGPVYSMIKSGARGSWAQLTQILGMKG
                     LVTSPNGEIIELPIKGNFKRGFDVLEYFIATHGVRKGLSDTALRTANAGYLTRRLVDV
                     SQDIIIREEDCGSHEGLLITKAESELIGRTLLRRITGRYLNADLKNSANKILAKKGDL
                     IDEELATKLADEDILEAEIRSVLTCNTRKGLCQKCYGHDLAYNKIAKLGTSAGVMAAQ
                     SIGEPGTQLTMRTFHTGGVASQDDITQGLPRVEEIFEARSPKRKAYIAEVAG"
BASE COUNT          952 a          595 c          774 g          979 t
ORIGIN      
        1 tctggcttta gatattcagc tattagatgg ccatggggag gtggttgaat taaccgatca
       61 gactgatgac tatgacagtc gttataacaa taataataat ttggatgcca atgctcatat
      121 taatgaagtg gttggtgaag aaatggctcg tcggtccagc caggcattaa cggaagatgc
      181 ccaatcagcc ggtttggaaa ttatttaggt ttttgattaa tcgtcttata tgttaaatcc
      241 aatcaaaact accgattttg aggctattaa attaaaacta gcctcgccgg aagaaattat
      301 cgcctggtct catggcgaag tgactcgacc ggaaacaatt aattatcgga cgcaaaaacc
      361 agaaaaagat ggcctgtttt gcgaacgaat ttttggtcca tccaaggatt gggaatgcta
      421 ttgtggtaaa tacaagaaag ttcgttataa gggaatcgtt tgcgataaat gcggtgttga
      481 agtaacccgc agcagcgtcc gccgcgagcg aatgggtcat atctcgctac aaacgccggt
      541 ctgtcatatt tggttcttgc gcggtctatc gtcaaaaatc ggtctagctt tgaatatttc
      601 cgttcaagcg attgaaaaag taatttattt cgccagtttt atcatcactg atgttaatca
      661 agaattgtta gaagctacta ttgagcagat tcgccaggaa tataagagca agaagaaatt
      721 gtttgagaat gaattcgcta aaggtttagc tgagttggaa aaacaaaaac aagccgcttt
      781 agctaacggt aaaaccgctt tagaggttaa acagatgatc gatcaatcac tagaagagct
      841 gactgccgat cgcgatgcca agattagtga gctcgaagaa gccttggctc aaactcagaa
      901 agagcttaaa gatttgcggc cgatgacgat tttaccggaa aatgtttatc agaatctgag
      961 cttaaaatat ggtcatatct ttactgctag tatcggcgcc gaaggtatcc gcgaattatt
     1021 agccaatatt gacttagaaa agattatcca ggagttggag gccgagctgg ccaccgccat
     1081 tgattctaaa aaagagaaaa ttgttaagcg tctccgtttc tttaagaatt tattagctaa
     1141 taatttgcgt cccgagtgga tgattcttaa ggttattcca gttatcccgc cagatttgcg
     1201 gccgatggtt gctttggatg gcggccgttt cgctacttct gacttgaatg atctctatcg
     1261 ccgcattatc aatcgcaata atcgcttaaa acaattgata gaattaaaag cgccggaagt
     1321 tatttgccgc aatgaaaaac ggatgttgca agaggcggtt gacgctttga ttgataattc
     1381 tattcgtcat ggtaaaacgg ttaccgcttc caccggccaa aaacggactc ttaaatcatt
     1441 gtcggattcg cttaagggca agcaaggtcg cttccgtcaa aatttattag gtaagagaac
     1501 tgactattcc ggccgcagcg ttattactgt taacccgaac ttacgactat ggcaatgcgg
     1561 tttgcccaaa agaatggctc tagaattatt taaaccgttt attatcagtg aattgattaa
     1621 acgggaggtg gtgcataatg tccgtagcgc ctctcgttat attgaggccg atcatgatga
     1681 tgtctgggaa atattagaaa atattgttcg tgaggcttat gtcttgctaa atcgcgctcc
     1741 cacattgcat cgtctaggta ttcaggcttt taagcccata ttaaccgaag gattgtctat
     1801 tcaaattcat cccttagttt gttcggcttt taatgctgac tttgatggcg atcaaatggc
     1861 ggttcatgtg ccattgactg aagaagctcg agccgaggcc cgtgatatca tgttggcctc
     1921 tcacaactta ttaaaaccag ccactggtga tccgattgtc aatcccggcc aagatattgt
     1981 ttggggtgtt tattatatta ctagtgaaga aaaaggagcg gaacaggatg ttaccaaaat
     2041 gaaggccttt ggttcttggc aagaggctaa attagcttat gattgtggta aaattagatt
     2101 gcgtgaaacc attcgggtac aaattgatta tttaaataat caattgatta ctacgaccgt
     2161 tggccgcatc gtttttaata atattttgcc ggagaaatta cgttttgtta atgagattgt
     2221 cttggggccg aaggttaaag ccttggttaa agattgcctg cgtcattatg gcgaagaggc
     2281 gacagttgat tttttggatg aattaaaacg agaaagtttt aagtttatta ctcgtagcgg
     2341 tttatcttgg ggcatgggcg atctgccgac ttttgccagt cgcgatgaac taattaagca
     2401 agctgagcaa gaagctgaag aggttcaaca gcagtatgaa gatggtttat taaccgacag
     2461 tgaacgttat ggcaaaatta ttcaactgtg gacagcggct aaagataaga ttaccgctat
     2521 ctgtatggaa gatttggatc gcgatggtcc ggtctattca atgattaaat ccggcgctcg
     2581 cggatcatgg gctcagctga ctcaaattct gggcatgaaa ggtttagtga cttcacccaa
     2641 tggcgaaatt attgaattgc cgatcaaggg taattttaaa cgcggttttg atgttttgga
     2701 atactttatc gctactcatg gtgttcgtaa gggtctatct gatactgctt tgcgaaccgc
     2761 caatgccggt tatttgactc gtcgtttggt tgatgtttcc caagatatta ttattcgtga
     2821 agaagattgc ggcagccatg aaggcttatt aattactaag gctgaaagtg aattgattgg
     2881 ccgaacatta ttacgtcgga ttaccggtcg ctatcttaat gctgatttaa aaaattccgc
     2941 caataagatt ttggctaaaa agggagattt aattgatgaa gaattggcca ctaagttagc
     3001 tgatgaagat attttggaag ctgaaattcg ttctgttttg acttgcaaca ccaggaaagg
     3061 tttgtgtcaa aagtgttatg gtcatgattt ggcttacaat aaaattgcca agcttggtac
     3121 ctcggccggc gtaatggccg ctcaatctat tggtgaacca ggcacgcagt tgactatgag
     3181 aacttttcat accggcggtg tagctagtca agacgatatt acccaaggct tgcctagagt
     3241 tgaagaaatt tttgaagctc gttcgccaaa acgtaaagct tatatcgctg aagttgctgg
//
LOCUS       DAOPAI010000039         2911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_26983_length_2911_cov_3.675070, whole genome shotgun sequence.
ACCESSION   DAOPAI010000039 DAOPAI010000000
VERSION     DAOPAI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26983_length_2911_cov_3.675070"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKV49_03420"
     CDS             <1..362
                     /locus_tag="PKV49_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22687.1"
                     /translation="LGLTSYVLINHNRQPISTPATATITASQQQPAELIFYYGDTCPH
                     CHIVDTYIKDNGISQKLKITQKEVYNNQENANELRDKAQQCGIPINALGVPLLWNGED
                     CLIGDQSIIDFLNEQIQ"
     gene            369..875
                     /locus_tag="PKV49_03425"
     CDS             369..875
                     /locus_tag="PKV49_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22688.1"
                     /translation="MNKKIGLLSLSALLTALIAKPAAAVCPVCTIAVASGVGFSRYLG
                     IDDTITGLWIGGLTVSIIAWTIDWLKRKNIHFWGRNWLVIIAYAAIVIGPLYFYGMFN
                     NDNCICGVNKLLLGLINGATGFWAGAEWYTYLKVRHNNRAYFPFQKVVMPIAPLIILS
                     LVFYWLLK"
     gene            882..1415
                     /locus_tag="PKV49_03430"
     CDS             882..1415
                     /locus_tag="PKV49_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22689.1"
                     /translation="MKNNKIANLNEFIAKIDAMKKQDKMDLSSDQDLTIAIMNLVGIE
                     EHFFFSGAKTGKTAYYDLINEVREMRKELLKKIIKEYEGEVWCISKHLLAASYRLMEV
                     GTKQLSAGKKEEAYSFFNKAYELYSLFWGLNMKLIATDHIKKIDDQAINKQDQSKQSL
                     ISKLGAIVKKAIDCCIE"
     gene            complement(1416..2090)
                     /gene="gpmA"
                     /locus_tag="PKV49_03435"
     CDS             complement(1416..2090)
                     /gene="gpmA"
                     /locus_tag="PKV49_03435"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPL22690.1"
                     /translation="MLQIVLLRHGESVWNQQKLFTGWTDVDLSDLGREQAKTAGQKLK
                     AAGFSFDLGFTSCLKRTKLTMELVLQAMGQDIIWRADWRLNERHYGNLQGLNKEQMAE
                     QFGAEQVKLWRRSYDVRPPAISDDNQYNQKNDPQYKGIVVPSAESLADVVVRVVPFWQ
                     EVIVPLLKNNQRIIIVASGNSLRALIKYLDQISPSEIVELEIPTGQPLVYELDEQFQP
                     IKHYYL"
     gene            complement(2122..>2911)
                     /locus_tag="PKV49_03440"
     CDS             complement(2122..>2911)
                     /locus_tag="PKV49_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22691.1"
                     /translation="LIIALPFLVSAGNLLAFIFSYKILVLLAFGLSGYLFSRYRRQLN
                     LPSYLDWFFWLNPALIINWLTEGHQEIFIVLILLWLLSSQSKSHCHQLGLLSLGFIKI
                     IAIILWPLSWFKNQIFNWRRFWLGGILLAATWLLFFAIIGLTPLDFWHKNLAFTDQHC
                     FYACSPLVMATNQLPAGIADVSRLILLIGAYLATLYCCLFKKYQPLKFIVWSLLALFF
                     IHTKWLTPWYPTLIIPFTLLIKERKYLVIGGFLTAYCLWHYLGL"
BASE COUNT          967 a          600 c          558 g          786 t
ORIGIN      
        1 ccctagggct gactagctat gtcctaatca atcataaccg ccaaccaata agcactccag
       61 caacggcaac gataaccgcc agccaacaac aaccggctga attaatattc tattacggcg
      121 atacttgccc acattgccat attgttgata cttatattaa agataacggc attagtcaaa
      181 aactgaaaat aacccagaaa gaagtctata ataatcaaga aaatgccaat gaactaagag
      241 ataaagcgca acaatgcggc atacccatca atgccttagg tgttccttta ttgtggaatg
      301 gcgaagactg tttgattggt gatcaatcaa ttattgattt tttaaatgaa caaatccaat
      361 aatcgcttat gaataagaaa atcggactat tatcgcttag cgcgctacta acagctttaa
      421 tagccaagcc agccgccgcc gtctgtccgg tctgtacgat tgccgtggct tctggtgttg
      481 gcttttctcg ttatctcggc attgatgaca ctattaccgg gctatggatt ggcggcttaa
      541 ctgtttcgat catcgcctgg actatagatt ggctcaaaag aaaaaatatt catttctggg
      601 gccgtaattg gttagtaatt attgcctacg ccgccatagt tatcggtccg ctatattttt
      661 atggaatgtt taataacgac aactgtattt gtggtgtcaa taaactatta ctgggattaa
      721 ttaacggcgc aaccggcttt tgggccggcg ctgaatggta tacatatcta aaagtcagac
      781 ataataatcg agcctatttc ccttttcaaa aagtcgtaat gcccattgct ccgctgatta
      841 ttttgagctt agttttttac tggctactta aataaaaata tatgaagaat aataagattg
      901 ctaatcttaa tgaatttatt gccaaaatag acgccatgaa aaagcaggac aagatggact
      961 tatcgtctga tcaagacttg accatcgcca ttatgaattt ggtgggcatt gaggaacatt
     1021 ttttcttttc cggcgctaaa actggcaaaa ccgcatacta tgacttaatc aacgaagtgc
     1081 gggaaatgcg caaagaatta ttgaagaaaa taatcaagga atatgagggc gaagtctggt
     1141 gtatttccaa acacttatta gctgccagtt atcgtttgat ggaggttggc actaaacaat
     1201 tatcggccgg caaaaaagaa gaagcttata gtttttttaa taaagcctat gaattgtatt
     1261 ctctcttttg gggcttaaac atgaaactaa tcgccactga ccatattaaa aaaattgatg
     1321 atcaggcgat taataaacaa gaccaatcta agcaaagctt aattagtaag ctgggcgcca
     1381 tagtcaaaaa agcgattgac tgctgtattg aataattata agtaatagtg tttaatcggc
     1441 tgaaactgct catcgagctc ataaactaac ggctggccag tcggtatttc taattccact
     1501 atttcgcttg ggctaatctg atctaaatat ttgatcagag ctctaaggct attgccgcta
     1561 gcgacgatga taatccgctg attatttttt agcaacggca caataacttc ctgccagaaa
     1621 ggcacaactc gaacaacgac atcagccaag ctctcggcgc tgggcacaac gattcctttg
     1681 tactgtggat cgtttttttg attatactgg ttatcgtcac taatggccgg cggccgcaca
     1741 tcatagctgc gccgccagag cttaacctgt tcagcgccaa attgttcagc catctgctcc
     1801 ttgttgagtc cttgcaagtt gccatagtga cgttcattaa gccgccagtc ggctcgccaa
     1861 ataatatctt ggcccattgc ctgcaatacc aattccatag tcagcttagt ccgctttaaa
     1921 caggaagtaa agccgagatc aaaagaaaat ccagccgctt ttaatttttg gccggcagtt
     1981 ttggcctgct ctcgacctaa atcgctcaaa tcaacatcgg tccagccggt aaataatttt
     2041 tgttgattcc aaacgctttc accgtgacgc aataaaacaa tttgaagcat aatcttaaat
     2101 tactaataaa aactatacta attataagcc aagataatgc cagagacaat aagcagttaa
     2161 aaaaccgcct ataacaagat atttacgctc ttttatcaac aaagtaaaag gaataattag
     2221 ggtcggatac caaggggtta gccacttggt gtgtataaaa aatagcgcca ataaagacca
     2281 gacaataaac ttaagcggct gatacttttt aaataaacag caatatagcg tcgccagata
     2341 agctccgatc aataaaatta accgcgatac gtcagctatg ccagctggca gctggttagt
     2401 ggccataact agcggtgaac aagcataaaa acagtgctga tcagtaaagg caagattttt
     2461 gtgccaaaaa tctaatggcg tcaggccaat gatagcaaaa aacaatagcc aggttgccgc
     2521 caataaaatg ccgcctagcc aaaaacgccg ccaattaaaa atttggtttt taaaccagct
     2581 cagcggccac aaaataatag ctattatttt aataaaaccc aaagacaata accccagttg
     2641 gtgacaatgg cttttggact gactagataa taaccataac aaaatcagaa caataaaaat
     2701 ttcttgatgg ccttcagtga gccaattaat tattaaagcc gggttcagcc aaaaaaacca
     2761 atccagatag ctgggtaagt taagctggcg ccgataacga ctaaataaat agccgctaag
     2821 gccaaaagcc agcaaaacta atattttata gctaaaaata aaagctaata aattgccggc
     2881 tgaaactaga aacggtaaag caatgattaa t
//
LOCUS       DAOPAI010000040         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_47051_length_1920_cov_5.582842, whole genome shotgun sequence.
ACCESSION   DAOPAI010000040 DAOPAI010000000
VERSION     DAOPAI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47051_length_1920_cov_5.582842"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKV49_03445"
     CDS             complement(<1..333)
                     /locus_tag="PKV49_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPL22692.1"
                     /translation="MSKFVIQGQSPLTGQINISGAKNSALKIIPASLLSEETITISNL
                     PAIEDIKLSLEIIRAVGGMVNRQDHQCSISTKNIQTTSIPPELANKFRASIMFVGPIL
                     ARTGEVSFP"
     gene            441..1712
                     /locus_tag="PKV49_03450"
     CDS             441..1712
                     /locus_tag="PKV49_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPL22693.1"
                     /translation="MVSSSTPRRKWRWLLVILLLFVAIAIGFWVGVIYNGKAQIVRRL
                     SDPEIVFLGKISGLYNSDTGGRLTHDLDFNLFWQTWDMVKTGYVDHDQLTDKQMFYGA
                     LRGLVSSTKDPYTIFMDPEDAKMFNEDLAGSFSGIGAEVGLRNDVITIIAPLADTPAE
                     RAGLRAGDKVYAIDQQSTAGLTVDEAVKKIRGPQGTEVTLTIGRGDAKLQDITIVRDV
                     IVVKSLDYSYNSKDGLFTIIINNFNADTETLFAEAVADIKVKNPKGLIVDLRNNPGGY
                     LDSAVAIASYWIESGVIVSEQSATEPPRQQMARGLAPLANLPTVVLINQGSASASEIV
                     AGALMDYGQAKTIGKTTFGKGSVQMVNDLPDGSMIKITTAKWLTPSGVSINDKGITPD
                     IEVDFTEADFNANRDPQMEKAKEFLLNQAGK"
BASE COUNT          533 a          335 c          429 g          623 t
ORIGIN      
        1 ggggaagctg acttctccag tgcgggccaa tatggggccg acaaacataa ttgaagccct
       61 aaatttgttg gctagttcgg gcggaatgct agtagtttgg atatttttag tgctgataga
      121 acattgatga tcttggcggt ttaccatccc acccacggct ctaataattt ccaaagataa
      181 tttaatatct tcaatagctg gcagattgct aatagtaatc gtttcttcgg atagtagact
      241 agcagggatg attttgagag ctgagttttt agctccgcta atgttaattt gtcctgtcag
      301 cggtgactgt ccttgaatta caaacttact cataatataa gttaaaagat ttattgattg
      361 tgattataat catagaccac tttgttgatt ctttcaactt gtgctacact gactacagtt
      421 aaaaaatatt ttattgctat atggtttcat catctactcc acggcgtaag tggcgctggc
      481 tattagttat tttgctatta tttgtagcca tagctattgg tttttgggtg ggcgttattt
      541 ataacggaaa agcgcaaata gtccgccgct tatctgatcc ggaaatagta tttttgggta
      601 agattagcgg attatataat tctgacactg gtggacggtt gactcacgac ctggacttta
      661 atttgttttg gcagacttgg gatatggtca aaactggtta tgtggatcat gatcagttaa
      721 cagacaagca aatgttttat ggagctctgc gaggcttagt tagctcaacc aaagatcctt
      781 atacaatttt tatggacccg gaggatgcaa aaatgtttaa tgaggaccta gctggcagct
      841 ttagcggtat tggagctgaa gttggcttgc gtaatgatgt tataaccatt attgctcctt
      901 tagccgatac accggctgag cgtgctggtc ttagagctgg cgataaagtt tatgccattg
      961 atcaacaatc tacggccggg ttaacggttg atgaggcggt taaaaaaatt cgcgggcctc
     1021 aaggaacaga agtaacccta accattggac gaggtgatgc taaattacag gatattacca
     1081 ttgtccgtga tgttattgtc gttaaaagtc ttgattattc atataactct aaggatggtc
     1141 tttttaccat tattattaat aattttaatg ccgataccga aacgcttttt gctgaagctg
     1201 ttgcagatat taaagttaaa aaccccaagg gccttattgt agatttacgc aataaccctg
     1261 gcggctattt agatagtgcc gtggctattg ctagctattg gatagagagc ggagtaattg
     1321 ttagcgagca gtcagctact gaaccaccgc gtcaacagat ggcccgtggt ttggctccgc
     1381 tagccaattt acctaccgta gtcttaatta accagggtag tgcttcagcc tccgaaattg
     1441 tagctggtgc tttaatggat tatggacagg cgaaaactat tggcaaaact acttttggca
     1501 aggggtcagt ccaaatggtt aatgatttgc cagatggttc catgattaaa atcactacgg
     1561 ctaaatggtt aacaccgagt ggcgtttcta ttaatgacaa aggcattact cccgatattg
     1621 aagttgactt tactgaggca gactttaatg ctaatcgtga tccgcaaatg gagaaagcta
     1681 aagaattttt attaaatcaa gctggtaaat agttttatta ttttaactaa caaaaagcac
     1741 cactagtgag tggtgctttt ttattgaata tgatatttct attcttctac atggataatt
     1801 tttgttgatt gtaggtgatt atcaaaacgg cgtatttttt tagtttgtat tttaacctgg
     1861 ccggccttag tagcaaataa agtatcatca tgaccgacgg ctacgttttt gccggctcgg
//
LOCUS       DAOPAI010000041         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_51844_length_1797_cov_4.013777, whole genome shotgun sequence.
ACCESSION   DAOPAI010000041 DAOPAI010000000
VERSION     DAOPAI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51844_length_1797_cov_4.013777"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PKV49_03455"
     CDS             <1..884
                     /locus_tag="PKV49_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPL22694.1"
                     /translation="IAEVAGLVTVENAQKTIDPVTGKVIGGGANKVVKISYQGEDVDK
                     YYFFDDSKTSKTKAATDELKIAVKDGDIVGAETKLFSLGRKVTKATRPGKVSLGKNYV
                     AVTVELPKEKEYIIPKDFALVVQSGQTVEVGDQLTEGSLDLRQLFELKGQIAAQKYII
                     QEIQRVYSSQGQALNDKHIEIIARQMFSRVYITDAADSDFTNGEVVERSIFDQVNEDL
                     KKAKKQPAKGQVLLLSMTKTALTTESFLSSASFQETSRVLIEAAISGKVDKLEGLKEN
                     VIIGRPIPAGTGITSTV"
     gene            complement(929..>1797)
                     /locus_tag="PKV49_03460"
     CDS             complement(929..>1797)
                     /locus_tag="PKV49_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPL22695.1"
                     /translation="TSSDGENTFFYIPWIGEYLAWLYNYSIGIIAVLALIAIIVGGFY
                     WLLAGGNASRVTEAKNWINAAFSGLGLALGSYLILVTINSNLVRFSSIKMMANKKIDI
                     PILEGGSESADNLVDTSNLSADCHDPCVPPSTLVSIKNTIPALDCVASDCRVNFTTYQ
                     KLITASSKAAANNYRLKVTSANRSCATQQKLWIQSGKNSSRVARPSCRSPHLSGSAVD
                     ITITDKKGANLVNKSYKELYPRNGPYNLGDNDKLTLLKRIMIESGFVRYCREWWHFQV
                     IKAAIPCYDPIK"
BASE COUNT          541 a          337 c          377 g          542 t
ORIGIN      
        1 atatcgctga agttgctggt ttggtaaccg ttgagaatgc tcaaaagacc attgatccgg
       61 ttaccggtaa agtgattggc ggtggagcta ataaagtggt taagattagt tatcaaggtg
      121 aggatgttga taaatattat ttctttgatg acagcaaaac tagcaagacc aaggcggcaa
      181 ccgatgaact taaaatcgcc gttaaagacg gtgatatcgt cggggctgag accaagcttt
      241 ttagtcttgg tcgtaaagtc accaaggcga ctcgccccgg taaagtcagt cttggtaaga
      301 attatgtcgc tgttactgtt gaactaccca aggagaaaga atatattatt cctaaggatt
      361 ttgctttagt tgttcaaagc ggccaaacag ttgaggttgg tgatcaattg actgagggta
      421 gtttggattt gcgtcagtta tttgagctca aaggccaaat agctgctcag aaatatatta
      481 tccaagagat tcagcgggta tattcatcac aaggacaggc cttaaatgat aagcatattg
      541 aaattatcgc ccgtcaaatg tttagccggg tttacatcac cgatgcggct gatagtgatt
      601 ttactaatgg cgaggtggtg gagcggtcaa tttttgatca agtcaatgag gacttaaaga
      661 aagccaaaaa acagccggct aaggggcaag ttttattgtt aagcatgact aagacagcgc
      721 taaccactga aagcttctta tcatcagcct cctttcaaga aacttcccgt gttttgattg
      781 aagctgctat tagtggcaaa gttgataagc tggaaggtct taaagaaaat gttattatcg
      841 gtcggccgat tccggcggga acgggaataa cttcaaccgt ttagataaat aaaaacaacc
      901 actaaatggg tggctgtttt tatgtctact attttattgg gtcatagcat ggtatagccg
      961 ctttgataac ttgaaaatgc caccattctc tacaataacg aacaaatccc gactctatca
     1021 taattctttt taataatgtt aatttatcat tatcacccag attataagga ccatttcttg
     1081 ggtatagctc cttataagat ttattaacta aattggcgcc ttttttatca gtgatagtta
     1141 tgtctacagc cgaaccgctt aaatgcggag atcggcaact tggtcgagct actctacttg
     1201 aattttttcc agattgtatc cagagttttt gctgagtggc acagcttctg ttagcgctag
     1261 tgaccttaag tcgataatta tttgctgcgg cttttgagct tgccgtaatt aatttttgat
     1321 aagtagtaaa atttacccga caatcactag ccacacaatc gagagccggt atagtattct
     1381 taatagacac caatgttgac ggaggcacgc atggatcatg gcagtcggct gataagttgg
     1441 aggtgtcgac aaggttatca gcgctttcac ttccaccttc taatattggt atgtctattt
     1501 ttttattagc catcattttt atactagaaa atcttaccaa attgctattg atagtcacta
     1561 agattaaata tgagcctaat gctagcccta gaccgctaaa ggcggcgtta atccaatttt
     1621 tagcttcagt tactcgacta gcattaccac cggccaataa ccagtagaaa ccgccaacta
     1681 taatggcaat taaagccaaa acagctataa ttccaatcga ataattatat agccaagcta
     1741 aatattcgcc aatccaaggt atataaaaga aggtgttttc tccatcactg ctagttt
//
LOCUS       DAOPAI010000042         1610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_3515
            NODE_60974_length_1610_cov_3.861736, whole genome shotgun sequence.
ACCESSION   DAOPAI010000042 DAOPAI010000000
VERSION     DAOPAI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865250
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 694
            CDSs (total)                      :: 658
            Genes (coding)                    :: 654
            CDSs (with protein)               :: 654
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60974_length_1610_cov_3.861736"
                     /isolate="AMR_MDS_3515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKV49_03465"
     CDS             complement(<1..403)
                     /locus_tag="PKV49_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPL22696.1"
                     /translation="MNFEKINNHQELIIDRFADLRGRNLSQQNLSMVSADILKTAEFD
                     TATIWPKKEKLPTAFNPEKLLLDGKNPGLGIKKLHQQGIDGRGVVVAILDQGLDINHP
                     EYKDSLIDYQTRGIDSEPISMHGPAVASLLVG"
     gene            complement(535..1470)
                     /gene="miaA"
                     /locus_tag="PKV49_03470"
     CDS             complement(535..1470)
                     /gene="miaA"
                     /locus_tag="PKV49_03470"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPL22697.1"
                     /translation="MAQLKAIAVIGPTAAGKTKLAIALADRYNGEIISADSRQVYRGM
                     DIGTGKDLSDYANNGRPVAYHLIDIADPQQIFDLATYQKMAQQALLDINQRHKLPILA
                     GGSGLYLQALIDNYQLSAVKPDNQRRQALEELSAEQLYEQLLTAAPALAQKLNNSDRH
                     NPRRLIRYLEIATGVKNQPSQPINAQSAIDWLILGIDYPMEVLEKRIYQRLIQRLENE
                     DMIQEVHNLHEQGLDWPRLESFGLEYRYISRYLREMIDYDQLVEQLHTAIRQFAKRQK
                     TWFKRWTKQGRKIHWLNTDNPLQEAEKLVENFLKS"
BASE COUNT          409 a          325 c          372 g          504 t
ORIGIN      
        1 taccaaccaa taaactggca acagccggac catgcatgct gattggttcc gaatcaatac
       61 cgcgggtttg gtaatcaatt agcgagtctt tatactctgg gtggttgata tcaagcccct
      121 gatcaaggat ggccaccact acgccgcgac catcaattcc ctgttgatgt agtttcttaa
      181 tacctaaacc gggattcttg ccatctagga gaagtttttc tggattgaaa gcggtcggca
      241 gtttttcttt ctttggccaa atcgtggccg tatcaaactc agctgttttt aaaatgtcgg
      301 cggaaaccat tgataaattt tgctgagata gattgcgtcc tctcaggtca gcgaatcggt
      361 caatgattaa ttcctgatgg ttgttaatct tttcaaagtt catatcgcgc ttttaataag
      421 aaaacaatgt ctgatgccgg gtttattagg catatattta ctaaaaaggt cttttaaatc
      481 ccttttcagt tagattatat gccaattagt tgattgtagc aataggccga gcgcttaaga
      541 ttttaaaaaa ttttccacca acttctcggc ctcttggagc ggattatcgg tattaagcca
      601 atgaatcttc cgaccttgtt tggtccagcg cttgaaccaa gttttttggc gtttagcaaa
      661 ttggcggata gcggtatgta gctgttcaac cagttggtca taatcaatca tttctcgcag
      721 atagcggcta atataacgat attctaaacc aaatgattct agtcgtggcc aatccagtcc
      781 ttgctcgtgt aaattgtgga cctcctgaat catatcttcg ttttctaggc gttgaattag
      841 tcgttgataa attcgttttt ctaatacctc catagggtaa tcaataccca aaattaacca
      901 gtctatagcc gattgagcgt taatcggttg agacggctga tttttgactc cagtagcgat
      961 ttccaaatag cgaatcaaac gccgcggatt gtggcgatcg ctattattta atttctgagc
     1021 taaagccggc gcggctgtca gcaattgctc gtaaagttgt tcggccgata attcttctaa
     1081 agcttgacgg cgctgattat ccggtttgac ggcgcttaat tgatagttat ctattaaggc
     1141 ttgtaaatat aggccgctgc caccggccaa gattggtaat ttgtggcgtt ggttaatatc
     1201 tagtagggct tgttgggcca ttttttgata agtggccaga tcaaaaattt gttggggatc
     1261 ggcgatatca ataaggtggt aagcaaccgg tcggccgtta ttggcgtaat cagatagatc
     1321 tttgccggtg ccaatgtcca tgccgcgata aacttggcga ctatcggcgc tgataatttc
     1381 gccgttatag cgatcggcca aggcaatggc tagtttggtc ttgccggcgg ctgttggtcc
     1441 gataacggcg atcgctttga gttgagccat aaaaataatt tattttataa taattgtccg
     1501 gccagactga aattacgggc agcggtcact tgggctaagt agcgttgtcc taaaacataa
     1561 ttattacccg tctggggtat tagcagaatc tgctgattgc gattggtgcg
//
