LOCUS       DAOPBK010000001        88105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_292_length_88105_cov_5.914957, whole genome shotgun sequence.
ACCESSION   DAOPBK010000001 DAOPBK010000000
VERSION     DAOPBK010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 88105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 88105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_292_length_88105_cov_5.914957"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..73)
                     /locus_tag="PLA17_00005"
     tRNA            complement(2..73)
                     /locus_tag="PLA17_00005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Arg,seq:cct)
     gene            complement(123..248)
                     /locus_tag="PLA17_00010"
     CDS             complement(123..248)
                     /locus_tag="PLA17_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83822.1"
                     /translation="MLFAAAPGGGSIVATGCSVVAVKVVKPPVKSKVKKKPAGGR"
     gene            complement(270..1952)
                     /gene="ettA"
                     /locus_tag="PLA17_00015"
     CDS             complement(270..1952)
                     /gene="ettA"
                     /locus_tag="PLA17_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPK83823.1"
                     /translation="MADDKKIIFSMYRVSKSYPPHKQVIKDISLSFFLGAKIGIIGLN
                     GSGKSTLLRIIAGLEKEYQGEVVFSPGYTVGHLSQDPLMDESRTVREIVEEGAAEVVS
                     VLREYEEINLRFGEPEVYENPDVMEKLMARQAALQEKIEALDGWNLEHKLERAMDALN
                     CPEPSTPVNILSGGERRRVALCRLLLLEPDVLLLDEPTNHLDAESVQWLETHLRQYKG
                     TVICITHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKARRLELEEKGSTKRRKT
                     LERELEWVRMSPKARHAKSKARLSAYENLLNQDVKQKEEKLEIFIPNGPRLGDRVITA
                     RGVAKAFGEKILFEDLDFCLPPNGIVGVIGPNGAGKTTLFRMIMGQETIDRGTFEIGE
                     TVTLGYVDQAHAAIDPGKSVYEVISGGQDEVLVGNRPVNARAYAARFNFTGADQEKKC
                     GVLSGGERNRLHLSLTLKSGANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVIISHD
                     RWFLDRVATHILAFEGDSKVVWFEGSFSEYDENLRRRTGNAGPVRFRYKKLS"
     gene            complement(2135..2569)
                     /locus_tag="PLA17_00020"
     CDS             complement(2135..2569)
                     /locus_tag="PLA17_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83824.1"
                     /translation="MEENAASSTVLTAEENRIKKHVNIAGILQIVFGSLLVFGALIIA
                     VVFGFINQFAEDPTAVKILAIISTPLVVLMLLFGAGMIAGGIGLFYCKRWARILTLVL
                     GGIGLLNIPLGTLKGVYIIWVLVQPEAISLFRKDVVVDLMQV"
     gene            2680..2907
                     /locus_tag="PLA17_00025"
     CDS             2680..2907
                     /locus_tag="PLA17_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83825.1"
                     /translation="MKKLLYASALLLITCLFNSCEILGDGCQVCQTVSYENGNAFAWG
                     TEAEYCGEELISIKAIPPSTVNGVTTVWECR"
     gene            3088..3492
                     /locus_tag="PLA17_00030"
     CDS             3088..3492
                     /locus_tag="PLA17_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK83826.1"
                     /translation="MLNITGKRIAIVEDDIASLKYYETLLKETGAEIITYRNGKKFVE
                     SVTTGREHFDLVFMDFLVPLVNGIECTRAFRQANRSAPVILITAYYSEQTKNEAYIAG
                     CTEYNLKPVFPEKIYQILEKYLTPRPAYQMYD"
     gene            complement(3557..6763)
                     /locus_tag="PLA17_00035"
     CDS             complement(3557..6763)
                     /locus_tag="PLA17_00035"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPK83827.1"
                     /translation="MIKKYRLWDNILGWTVFVVASVTYLMTIEPTMSLWDCSEFIATA
                     FKLEVGHPPGAPFFMILGRFFANFAGGDVSKVAMMINAMSGLASALTILFLFWSITHL
                     ARRIFLKQESDYTAGRIIAVLGAGLTGSLAYAFSDTFWFSAVEGEVYATSSLFTAVVF
                     WAMLKWEDVADEPHADRWIILIAFMMGLSIGVHLLNLLAIPAIVFIWYFRKYSFSWKG
                     FILSIAVSMLLLVLIMYGIIPGIVRMTSAFELFFVNNLGLPFNSGMYIHLLLFIVALM
                     LSVWYSFTHSDRLRIFILAGITLLLSGVWLLTGSLVLNIVFLAVIVYGLWLLSTKQKV
                     ALNTILTSLMVIVIGYSSFAVIVVRSSANPPINENNPSNPFALLYYLNREQYGRRPLF
                     HGAYYNAPVTDYVKGKPTYNPSGDRYIITNRETNRVYDERFMTVFPRMWSDSPDHVRE
                     YEKYVGTKGKPVRVTDPQTGEATTLRVPTFGQNLSFMFRYQLGFMYFRYFMWNFSGRQ
                     NDVQSIGGPVNGNWITGLDFLDAPRTGSVKGMPEDMRNDPSRNTYYLLPLLLGLAGFL
                     WHFNRDIRNWWIVLLLFVMTGIAIVIYLNQYPNQPRERDYAYAASFYAFTIWIGLGVL
                     ALFELFRKAAGETPSAIIATVLCFAAVPALMGSENWDDHDRSGRYLGREVAFNYLNSC
                     APNAIIFTGGDNDTFPLWYAQEVEGVRTDVRVCNLMLLNTDWYIDQMKYRAYTSDPLP
                     VSLQKELYYDGVNNQVAVFERVKGPATAKEVMEFISSGSEVSKLKLYGEDLWYIPTRT
                     IRIPVDSAKVLANGTVKPEDADKIVPYIDINLKGSWIMKNQLLVLDILAHNDWDRPIY
                     FVTGQTDDALGLEEYFQLEGLAYRLVPIKGVNKSWFEYGSIDTDILYENMMNRFSWLS
                     ANDPDVYIDYYHNRTLLVIRARLNHARLAQALVAEGDTARAVQVIDRCLELFPVSNVD
                     YDYYFGDIISACFASGMKEKAKQLTGEFTDYFAARTAYLLDQRPSVAYYAGAEIANGL
                     QMMLQAIRVCFDNGEMALAEEINGRYNELYARYAAFNQ"
     gene            6871..9978
                     /locus_tag="PLA17_00040"
     CDS             6871..9978
                     /locus_tag="PLA17_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83828.1"
                     /translation="MKRFVILLIMSLFSLARAGAQYYDTGQDPASLRWLQIRTPHFRI
                     IYPDDFGGEAFRYAHLLEESFEKLSLLYPGVRTNIPVIIHNHSMQSNGYVSWAPRRME
                     LYPLPGQDNLPMHPAAQLAVHETVHMLQLGSLNSRGFGRALRFILGEHAVGLSAVMIP
                     LWAFEGDAVYAETATTPSGRGRSNAFIRGAMALSLKPGGIYGYDKMLSGSYRNFTPNH
                     YVFGYLMMNHLRTIDPDAWNEVYRIASNGLPGNPVNAGLRKETRLTKRRLYDATFAAL
                     GKSWRESMPEGVKEYTPLSLPGKRNYVSHYTPHRMDDGRIISLRTSLSDPSRFVITGN
                     SGGKELNITTTGYIYPCFFSFSGNTIVWAEQYPDIRWDNRDYSVIKRLDLPDGLITDV
                     TSRTRYTAPDLSPDGRTIVAVSTTPDMVCSLVFLDSHTGEVLMDLVPPDGLILQRPAW
                     SSDGRQVTMVTLNQQGEGIRTYRPTGKKWTVNLEESHTDIVQAKIHNDTLFFLAQGDG
                     SDNIYRIAGSGPVERVTGSRFGISGFSVRGSELLFSDYTADGFVIASEKSSATAGPAF
                     MTGHEILPPVAPMPGEAPEKEPPQQVAPIPGTEPAYEQLPQVAHMPGAEPAKKSLPQA
                     APTPGAEPEKEPLPEITPNPGGAPAENILPDVTSPPADDSVSDATMPLIAEPGPYRKI
                     ANLFNPHSWLPFYADLDEIRTDPATIRPGLTLMSQNHLSTLISTVGYEYSEGNHYLHS
                     GITWKGWYPVIDAEIKWGGEQLIISDTSATLPPENPGTDLQLNLSIYDQLWFARGKFR
                     QMVMPALYISYRNRDTWLSDENRYDRDVLTLTGRFYFSNIFRTAYRDINPKWGQVFDL
                     QLTTTPWDTKLYSSKSYARTILFFPGALPNHSLSLRVGRENQAPARKHLYRNKLPWPR
                     GYDHNLVAEKLLSFSADYTMPLFYPDLAAGSFLYLKRIRGTLFYDYSKGVDIRNYADR
                     SFHAGPKRFCSAGSELMADFYLLRIPFEISAGIRAGYIPGENRYFVNGAFSVNIYGTV
                     LGRER"
     gene            complement(9937..11379)
                     /locus_tag="PLA17_00045"
     CDS             complement(9937..11379)
                     /locus_tag="PLA17_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HPK83829.1"
                     /translation="MDSNEFRRYGHEIVEWIARYFENVEQYPVKSGVRPGEIKNALPE
                     LPPSGSESFDLFMKDFDEVIMPGITHWQSPNFFAYFPANSSPPSVLAEMLTAALGAQC
                     MLWETSPAGTELEERMMEWLRSMMGIPPEFEGVIQDSASSSTLAALITAREKVTRYRS
                     NREGLSDTGRMRIYVSGQTHSSVEKGAGIAGFGRDNLVRVEVRSDFSMDPEALERAIE
                     RDIASGYIPCCAIATLGTTGVTAIDPLRAMGEICRKHGIWLHVDAAIGGTALLLPEMR
                     WMADGIEYADSLVFNPHKWMFTNFDCSVYFVRDAASLIRSFEILPEYLKTRTRGEVND
                     YRDWGIPLGRRFRALKLWAVIRSYGVEGLQSLIREHLRLAKMLAGLIEADRDFRLMAP
                     VTLNTVCFRYCPAGMGDEDANTLNEKINHALNDTGKIYLTHTKVDGRYVLRMVTAQTG
                     VTERHVRQAWDLIRETASALSSQNGAVDVD"
     gene            complement(11389..11637)
                     /gene="atpC"
                     /locus_tag="PLA17_00050"
     CDS             complement(11389..11637)
                     /gene="atpC"
                     /locus_tag="PLA17_00050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPK83830.1"
                     /translation="MKIEIITPDKNIYSGEIRSVRVPGRKGSFQVLKDHAPIISTLDA
                     GKVIIADDQGTEVRFEITGGVIEVKRNRIILLADSVVQ"
     gene            complement(11656..13164)
                     /gene="atpD"
                     /locus_tag="PLA17_00055"
     CDS             complement(11656..13164)
                     /gene="atpD"
                     /locus_tag="PLA17_00055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPK83831.1"
                     /translation="MAKKTGVISQVIGPVVDVTFDTAGTDMPNILDALEIKRHDGSVL
                     VVECQQHIGEKTVRAIAMDSTDGLQRGMEVVATGQPIIMPVGDQIRGRLMNVTGMPID
                     GIGPLDKKGGYPIHRKPPKYEDLSTEKEVLYTGIKVIDLIEPYSKGGKIGLFGGAGVG
                     KTVVILELINNIAKAYSGLSVFAGVGERTREGNDLLREMLEANVISYGKAFLEDMAKG
                     GWNIDLVDREELKESKLALVFGQMNEPPGARARVALSGLSVAEYFRDGEGEGTGRDIL
                     FFMDNVFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGVMQERITSTRHGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLSRKISELGIYPAVDPLDSTSRILTPEVVGEEHY
                     RCAQRVKEILQRYNELQDIIAILGMDELSEEDKLVVHRARRVQRFLSQPFHVAEQFTN
                     IPGTLVSIEDTIRGFNMIIDGAVDQYPEAAFMLVGTIEDAIAKGEKLIAESR"
     gene            complement(13240..14214)
                     /locus_tag="PLA17_00060"
     CDS             complement(13240..14214)
                     /locus_tag="PLA17_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPK83832.1"
                     /translation="MFTSEYLRELTNKAIASLGGTPEAERLFEPVRYILSIGGKRLRP
                     VMCLLSCNIFSDKIDEAVMPAVGIEVFHNFTLIHDDIIDKDEVRRGSPTVHAKWGPDQ
                     AVLSGDVMAFIAMECIGQAPERALPKVLKLFNRTAIEVCTGQQLDLDFEKKGVVSEAE
                     YLRMIELKTAVLIAASLKTGAIIGDASEKDQDLLYEFGRCLGIAFQIQDDVLDTYGDT
                     KLFGKKTGSDIVANKKTYLLVKAFEMAGGSMLTRLRELLGPGSSDPEAKVRGVRDIYD
                     ELEIRLQAENLAYDYFNRAFQALDQVSANGERKSELKQLSVNLIGRVK"
     gene            complement(14230..15117)
                     /gene="atpG"
                     /locus_tag="PLA17_00065"
     CDS             complement(14230..15117)
                     /gene="atpG"
                     /locus_tag="PLA17_00065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPK83833.1"
                     /translation="MANLKAIRIRLSSVRSTRQITSAMKMVAAAKLRRAQDAIVQLRP
                     YADKLNEIQASVCAALRDTETENIYAQRKKGNGKVLIVVVTSNKGLCGAFNANVLRET
                     RRIMDERYSQENSRGDLGVMTVGKKANDALRKLKCNIIEDQSDLYTELTFDRISALAD
                     KLMRLYVSKEYDTIEIVYNQFKNAAVQKLTNEVFLPVTMGTMSGQDSAPADYIYEPDQ
                     VRIISELIPKTLRIKLYRSLLDSFAAEHGARMTAMHQATDNATELIRDLTLQYNKARQ
                     ASITNQLLEVVSGAEALNG"
     gene            complement(15139..16725)
                     /gene="atpA"
                     /locus_tag="PLA17_00070"
     CDS             complement(15139..16725)
                     /gene="atpA"
                     /locus_tag="PLA17_00070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPK83834.1"
                     /translation="MTNIKPSEVSQILRQQLEGVETAIDVEEVGTVLQVGDGIARIYG
                     LKNVGSNELIEFVESNIKGIVLNLEEDNVGAVILGPSEKIKEGDIARRTGRISSIMVG
                     EGLLGRVITTTGDPIDGRGPITGKLYEMPFERKAPGVIFRQPVKQPVQTGLKAIDAMI
                     PIGRGQRELIIGDRQTGKTAIAIDTIINQRSEYEKGKPLYCIYVAIGQKGSTVANIAT
                     TLEQYGAMDYTVIVSATAADSAAAQFYAPFAGAAIGEFFRDTGRDALIIYDDLSKQAV
                     SYREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIIESDEVAARMNDLPESIRHMVK
                     GGGSLTALPIIETQAGDVAAYIPTNVISITDGQIFLESSLFNAGIRPAINVGISVSRV
                     GGNAQIRSMKKVAGTLKVDQAQYRELEAFSKFGSDLDAATLAVLDKGAKNVEILKQNQ
                     YSPVKVEEQVAIIYCGTQGLLRDVPVHRVSEFENEFLAYMRNNHSDVLETIKKGIIND
                     EVTGVLDKAVEEVASQFKSR"
     gene            complement(16747..17295)
                     /gene="atpH"
                     /locus_tag="PLA17_00075"
     CDS             complement(16747..17295)
                     /gene="atpH"
                     /locus_tag="PLA17_00075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPK83835.1"
                     /translation="MNSARIAVRYAKALFDLALESGTVDGVYRDMKVLSRLCAMEEVK
                     NVIRNPVLPQQKRKDAIIALAGDDAEKQTIDFINLMFSNGRGDYLSAAARNFIDLTRR
                     HKGIRQVTVTTAVPVDGTTKRELADLITGEKGGTIEFNEQIDESIIGGFILRVDDSYV
                     DASVRNRLNRFRKEFSLAGFTE"
     gene            complement(17300..17812)
                     /gene="atpF"
                     /locus_tag="PLA17_00080"
     CDS             complement(17300..17812)
                     /gene="atpF"
                     /locus_tag="PLA17_00080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPK83836.1"
                     /translation="MLLQSSLASPAIGTIFWTTLIFLLLLVLLWKFAWGPIMKAVKTR
                     EDMIKNSLESAEKAREEMKVLQADNEMILQKAREERDKILRDARVAYDRMMAEAREKG
                     QSESDALVRRARELIEREKVTAIAEVKKEVARLAIEVASKVVGETLKSDAEQQKLIER
                     YINEIEANRN"
     gene            complement(17878..18093)
                     /gene="atpE"
                     /locus_tag="PLA17_00085"
     CDS             complement(17878..18093)
                     /gene="atpE"
                     /locus_tag="PLA17_00085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPK83837.1"
                     /translation="MEQAVMSLAGVGAGLAVIGAGLGIGRIGGSAMDAIARQPEAYSK
                     IQAAMIIAAALIEGAALFAIVVAMIKG"
     gene            complement(18132..19208)
                     /gene="atpB"
                     /locus_tag="PLA17_00090"
     CDS             complement(18132..19208)
                     /gene="atpB"
                     /locus_tag="PLA17_00090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPK83838.1"
                     /translation="MKNRYLHLLLLVLGLLAGTVPAFSSGIMDKEGKAHGGEEEFDAA
                     PFIMDHVADSHEWHILTKKDGTSVAVYLPVIIYDRESGLHLFSSRRIAHGQSYKGFAI
                     AHEGKYAGKIIRVDQEGQHDGSKPPLDFSITKAIFGMIVAAIVLLWLMLSLARSYKRA
                     GTAAPKGFQGVVEPVILFIRDEIAIPNIGDKKHAKFMPYLLTVFFFILINNLLGLIPV
                     FPFGANVTGNINVTFVLALFTFLITQFSANKSHWRHVFAPPGVPFWLLPVMVPIEIIG
                     LFSKPFALMVRLFANITAGHIIVLSLVTLIFIFKTVAVAPFSVIFVLFMDCIELLVAF
                     LQAYVFTLLSALFIGLATAEPEHH"
     gene            complement(19205..19546)
                     /locus_tag="PLA17_00095"
     CDS             complement(19205..19546)
                     /locus_tag="PLA17_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83839.1"
                     /translation="MEMVVGRLFPSLFNISAAAFLTAGFTLTAFIALLIFFNGYHSDR
                     ERSVFMTLIALGLKMVLCFVLALLFFLVFKNKSTGSVVLFFILYLAITVYVVLTFTGV
                     LKKKSDLRNSA"
     gene            complement(19605..19865)
                     /locus_tag="PLA17_00100"
     CDS             complement(19605..19865)
                     /locus_tag="PLA17_00100"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPK83840.1"
                     /translation="MKTVEQQQPNPKNKVNRGKQGLDAYSRYSTIAIQMVIIIVITSL
                     GGVKLDKLAGTEPILTVVLSLLGVASAMWLVIKEVLRNKGDV"
     gene            complement(19816..20208)
                     /locus_tag="PLA17_00105"
     CDS             complement(19816..20208)
                     /locus_tag="PLA17_00105"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPK83841.1"
                     /translation="MAKIIETDNSNAVNLISQGTEITGDIKSTGDIRIDGILNGNMST
                     RGKVVIGPTGKVKGEVECKNSEVSGLIEGKITVSQLLNLKASSKINGTIITNKLSIEP
                     GAVFTGNCVMKEGENGGTAAAKPKEQGK"
     gene            complement(20326..21324)
                     /locus_tag="PLA17_00110"
     CDS             complement(20326..21324)
                     /locus_tag="PLA17_00110"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPK83842.1"
                     /translation="MTKIRAAITGINAWTPEYRLTNQELSGMVETTDEWIMQRVGIKE
                     RRILKGEGKGTSDLGEPAVRGLLEKTGTRPEEIDLLICATVTPDMAFPATANIISDKV
                     GIKNAFSFDINAACSGFLFALQTAAAYVETGRYKKVIVVGSDKMSSITDYTDRTTCTL
                     FGDAAGAVLLEPTYEELGVMDYIFRTDGSGRKYLHMKAGGSVRPASHDTVGGREHYIY
                     QEGQAVFRFAVSNMADVAVEIMEKNNLGPDDINWLVPHQANLRIIDATGRRMGLPPEK
                     VMINIQKYGNTTAATIPLVIWEFEKKLGKGDNLILAAFGGGFTWGSIWLRWAYDPA"
     gene            complement(21321..22265)
                     /locus_tag="PLA17_00115"
     CDS             complement(21321..22265)
                     /locus_tag="PLA17_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HPK83843.1"
                     /translation="MKIGLDAMGGDYAPNAVIEGAFDALGQMSSGDRIVLIGDNGRIR
                     EKLASLDADASLFDIVPTSQVIEMSDHPAKAFSQKRDSSIAVGYGMLKKGLINGFASA
                     GNTGAMMVGASYTVNVIPGVFRPALAALIPNINGKSSIILDVGINPDSKPDVLLQYGI
                     LGSVYAKHVLGVKEPTVGLLNIGKEKAKGSAAVKAAYELLSESSDVTFRGNVEGHNLF
                     TDEMADVIVCDGFVGNVVLKMAEKLYVVARSKGIKDSFFDRLNFEVVGGTPVLGINEN
                     VVVGHGISNRMAIMNMLLRTRDVVQADLAGKIREAFGA"
     gene            complement(22294..22479)
                     /gene="rpmF"
                     /locus_tag="PLA17_00120"
     CDS             complement(22294..22479)
                     /gene="rpmF"
                     /locus_tag="PLA17_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPK83844.1"
                     /translation="MPNPKHRHSATRRDKRRTHYKAVAPTTAACTNCGSAHLYHRVCP
                     ECGYYRGKLAVEKKTTA"
     gene            complement(22497..23018)
                     /locus_tag="PLA17_00125"
     CDS             complement(22497..23018)
                     /locus_tag="PLA17_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPK83845.1"
                     /translation="MPGLYSIPLAGLKEARYTYDFQIGNHLFGLYEESEIKKGEFRAV
                     VVLQKCSTHIEFDIVINGKAEVICDRCLEPFYMPVSTAKRLFVRQGSEWEESDPDMIT
                     MPMDENEIDLSQFFYDYIHLALPIKRIHPDDADGNSTCNPDMIRRLNDLLVHGEVQTD
                     PRWDDLKKLTGYN"
     gene            23200..23535
                     /locus_tag="PLA17_00130"
     CDS             23200..23535
                     /locus_tag="PLA17_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83846.1"
                     /translation="MKEKHGNPFTGIAKFLISLGIVMFVAKLDLLGLGDIPEYFSWQM
                     LLIFFGVWLLLSFKLVSAVIFFAIGFWFLMPDMAIQLTESYKKIYWPVVLILCGVAFM
                     LKPLAKSCR"
     gene            23694..24434
                     /locus_tag="PLA17_00135"
     CDS             23694..24434
                     /locus_tag="PLA17_00135"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPK83847.1"
                     /translation="MENELKKPARNPETSNRFIIGVLLIIAGLILIVKKSTVLPEPLD
                     DFIDNIIFSWQMLLIVIGIIVLNGSDNKTPGIVMISVGGFFLIPELFTDFFRSFNFFW
                     PALFIVLGIVLLVNSKRLVKKLDFSTVNKADYIDYVNVFSGAERQLITDNFRGGKITS
                     IFGGGEVDLTHSSLSPGDNVIEITCIFGGTTIIVPESWNVVIEITPILGGFSDSRKMR
                     GDVIRDNTRSLTIKGTVMFGGGEIKSYK"
     gene            24438..25496
                     /locus_tag="PLA17_00140"
     CDS             24438..25496
                     /locus_tag="PLA17_00140"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPK83848.1"
                     /translation="MKHPLLENRTRFFVWWLAWLILAAGQSLVIHFGYGIPADTAFAD
                     GVISMIFFGILGLAVWFPLRFLLKEDNQVYTTIVNVLLTGSLTILAWLFGSKIIVRML
                     VAEKVDYDIFWHSVLIFRATAGVLVFFVMILVYYLFLSSSRLAEKVARQAQLEAQVRE
                     GELKMLRSQINPHFLFNSLNSISSLTVTDPLKARDMIVKLSEFMRYSLSSRSEQPVTL
                     GNEMESLRLYLQIEKVRFGERLRIEEDINPECLPALMPGMLLQPLYENAVKHGVYEST
                     GEVIIRTTIRKQNEMVIITILNNVDTDSVVTRKGAGIGLKNVSSRLELFFGDKADLTV
                     NHGDDSFTVIVRFPFRKS"
     gene            25516..26268
                     /locus_tag="PLA17_00145"
     CDS             25516..26268
                     /locus_tag="PLA17_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPK83849.1"
                     /translation="MKTIRALIIEDEAPARELIRFMLADHPEVEIVGECVDGFCGARE
                     IKEKRPDLIFLDIQMPRLTGFELLEVIDEKPEVIFTTAFDQYAIRAFELNAVDYLLKP
                     FSKERFDDALARALNRLETKAPEHDKIEKLVAAASEAEEFLTRIVFRKGAGIKIIPLN
                     NVYYLAAEDDYVMIYYSEGKALKPKTMKYYEEHLPPDLFIRVHRSFIVNVEQITRLEP
                     YSKDNYVAVLRSGEKIPVSRAGYKMLREKLSF"
     gene            complement(26265..26579)
                     /gene="yidD"
                     /locus_tag="PLA17_00150"
     CDS             complement(26265..26579)
                     /gene="yidD"
                     /locus_tag="PLA17_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008564315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPK83850.1"
                     /translation="MLKSAAKILSFLLLLPVWLYRWIISPWTPASCRHVPTCSRYTID
                     ALRLHGPFRGIMISADRILRCRPGGTHGYDPVPMFIFRRYRGCRSLLCRAPRCNRLKD
                     HD"
     gene            26691..27395
                     /locus_tag="PLA17_00155"
     CDS             26691..27395
                     /locus_tag="PLA17_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPK83851.1"
                     /translation="METMFLQNGVYWIIFIVFMILSWVVSARLKSKFRTYSKVPVASG
                     MTGREVAEKMLRDSGVTDVTVHSVAGELTDHYNPLKKTINLSRDVYSGTSVAAAAVAA
                     HETGHALQHAQSYAWLGMRSKLAPVVSFASNWIQWILLAGLVLLNSRPEILLIGIVLF
                     ALTTLFSFITLPVEINASKRALAWLSDAGITSPGTDDKARDALKWAAYTYVIAALGSL
                     ATLIYYIMLFLGARDR"
     gene            complement(27424..28158)
                     /locus_tag="PLA17_00160"
     CDS             complement(27424..28158)
                     /locus_tag="PLA17_00160"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPK83852.1"
                     /translation="MSLVHELEKSGNWLFRRRSWLPATMLVPAAFYLFIANRSVIYFN
                     YTWEIICILVSVTGLLLRSYTIGYLPRSASERNLIDQMADELNTKGIYSVIRHPLYVG
                     NILMSLGPVLFLRSVWWVIVFVLASWLYYERIIFAGEQYLRRKFGEAYDTWAFRVKAV
                     IPSFRHYVKPDLPFSFRNVIRREYKSIANLFLVFAFLDLCRNIAVTGRIYLEPMYVTL
                     LVAALIFWAVIRYIIKRTKFLYVGGR"
     gene            complement(28356..30350)
                     /locus_tag="PLA17_00165"
     CDS             complement(28356..30350)
                     /locus_tag="PLA17_00165"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPK83853.1"
                     /translation="MEQVALYLLKSAAWLTAFTLVYLLFLQKERFFMLKRIYLLAGVV
                     LSLLLPLVTLHYKIELPAPLIPDGQGMTSASPAPAAQITEHADILKWFMIFYIAGILL
                     MSGRTLKYLLSVCRTVRSGQVDREGGVLLVRSEKNTFAFSFFNYVFINPSLSGREVRE
                     IINHEMVHLSQKHWFDLLLVELVSIVQWMNPFAWICSALVRQNHEHLADEEALQRTSD
                     PAAYRAALLNQVFNARLISLSNSFNFSFNTNRFEMMKKKNYSPYRKLKLLLVLPLVAV
                     MLYAFADPVYTYSDSAPGSLIKDIQGTVVKEDGTPLAGVTVNVSGTSLSVTTDETGRF
                     YFSDLPAYVGIMFTAAGYEPVALTAGSVRGATITMKANQRYANMGSVSQGLLVMVDGQ
                     ASDLSLEEATKKLGKDFGKSVYLDPAAAKTKYGDKGEKGAIEVTSRSKAKESGIILPY
                     PREDEADFPTFRGESFLSFADWVAERAKYPADAASRSVSGWVRMAYTVDTDGSVSGIK
                     AISDSDPALVGELTRVMESSPRWKSPANREADEPFTSEIVIRFQLPDRVSNGIVYIMA
                     DEMPQFPGGDTELFKWLYGTLKYPDQAKGKGIQGRVTLRFVVTSDGYVEDVSVVRGVD
                     PLLDDEAVRVMSQCPQWKPGKIKGNPVNVYYAIPIKFSLQ"
     gene            complement(30354..30728)
                     /locus_tag="PLA17_00170"
     CDS             complement(30354..30728)
                     /locus_tag="PLA17_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPK83854.1"
                     /translation="MQLTKAEEQIMQILWELEEGVVKDIRERFDDPKPARNTISTVLK
                     VLERKGYVDHRAYANVFLYYPLVSKREYSKHQLFGLLEGYFNNSFPAMTSFFAREKNL
                     TVKDLDRILEEAREELDDDQQE"
     gene            complement(30754..32226)
                     /locus_tag="PLA17_00175"
     CDS             complement(30754..32226)
                     /locus_tag="PLA17_00175"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPK83855.1"
                     /translation="MRELVVFISALVLLAGCNGGKEYSPEIDFGGVAYIKVIVQDDLD
                     SVRMDINHSSYYPAQSCLIHRFTLVHKRVCEARYRITRPEQVWFQFRDTVYTSYMIPG
                     DTLVARVGLDKAGDGSSNISFHIDDPIFAFLQNERREFGCFYTQSPIAITAFNTQPET
                     ERQFKDAVKRIDQAGKERLEFLQKNCNNLPGWFCDFYENEISYFSAHMKFFQYYYLKD
                     RNLKGIPMPCDVKIYNPEAVSSPIYWRFISDYLLLSDTVDDRLIGSPRLMAYYSKASH
                     NINSLLKGKVLEYFNVYLLYDLYYRFDTRREFQLADSFRVASGFRLTPLQESYIDERR
                     SESVKKLGKLEDENRLKPGSEAPYFYLKDTAGVFHTLSDYRGKLIYLHFWATWCGPCL
                     EEIPLLNTLAENLSGKPVEIINICLDDEYEKWKSIIENEKLRGTNLICTGNWAADFQE
                     KYAVRGIPHYAIIDADGLIISENCERPDKVYERLLTLIHK"
     gene            complement(32721..33824)
                     /locus_tag="PLA17_00180"
     CDS             complement(32721..33824)
                     /locus_tag="PLA17_00180"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HPK83856.1"
                     /translation="MKELIIVTLSIMLASCATVKQTSSPAEEHSAPSAGNLPAQAAAD
                     LSSPDTAYFPTLAVMELCTPTASNPPVSSAGNLPVPAVANSPSQFAADLPIPADADLP
                     IQTVANLHAAESHEDQDHLILATLWFRKSAEMRALFYQCFRNAEIALKENLELSVNNS
                     SGADPSTPDETAPTGHKKPAAVVLDIDETLLDNSPYQGWQILNRKAFNNDDWFRWVAL
                     ASARPLPGSVEFTRYADSLGVEVFYVSNRTVEEMGPTIANIAAWGFANADSTHMLLKE
                     SVSSKVERRAFIEKDYEIILLVGDNLADFSGIYEKRGPDHGFGAVDADRRLFGTRYIV
                     LPNPMYGNWLNDLLQSAPGSTEREKLLQIVEGF"
     gene            33974..35560
                     /locus_tag="PLA17_00185"
     CDS             33974..35560
                     /locus_tag="PLA17_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HPK83857.1"
                     /translation="MSTLKLMLGMIPSTSKIEQEEKALIAEYEKMIAFAGSEALAKYT
                     RLHESVNSAGFQQKRKEIESLTYKGSEEHAREAEFLGLQKAKDIVSFFKTEGSALLKN
                     FLSLDGSARIKEIEDLQLFVQSPEFKQKERMKPITFKHTEEYGKWLEFKKLKGDREVK
                     KNLNPEKVNRFTELKALVTSADFLLKKNMKPITFKDTPEYQKLQELKTKKSATDIVNF
                     YKFRKTKDYHNWVQVKDSARLKRYYELQEYLKTKEFHERKEYLLDKRRFEKSEMFREL
                     KEYETLKNSPEIKWYFSIKDSNKFDILKNRELTFSDEFEGTAPDSSKWINRYYPGDKL
                     LHDSYSISTDLHSYTPSDNIEVRHSVLRIITRPQKTNGKAWDPAKGFYTKEFGFTSGI
                     VNTGASFRQKYGTFTAKIKLTDPNVRNAFWLIGDRITPHVNICRSGSGKVWFDLFTSP
                     GSHYKKSIGSRYLSDFYIYTLDWTPNALVWKINGVEVMRQTTGVPQEPMFINLAGGVD
                     KPIGGISSMEIDWVRVYQPK"
     gene            35695..36180
                     /locus_tag="PLA17_00190"
     CDS             35695..36180
                     /locus_tag="PLA17_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK83858.1"
                     /translation="MTVKEFNNAVREFGDHVYRFILRSIKDSHRADDIVQDTYEKLWN
                     CVTEIEYGAVKSWLFTTAYNRMIDVIRKDSRLVDVDHYDDSTLYADENSSDLNEVLHR
                     ALEKLPAVQRSVILLRDYEGYSYREIGEITGLSEAQVKTYIFRGRVALRNYLVKTSVW
                     Q"
     gene            36171..37274
                     /locus_tag="PLA17_00195"
     CDS             36171..37274
                     /locus_tag="PLA17_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83859.1"
                     /translation="MAITEDMDFPRLTPPDDRMPSGSSLFRSPAELTDEQFDLLAAAW
                     AEGALSGESLEELESAMAASADRRDRAGSFCSIRLVPCDESWPGMRSAIRLSPVQRTL
                     RRSVIPTLLAVAAMVSMIIYGPTVLKLKTINSGKTITRSAMTVAEIQVPPPIVAPEKK
                     PATHTPSAQVAQTPVAEENALPGLLAEKNAMQNLPAEENVLRGHFAEVNALQGHPAEE
                     SALQGHFAEANTPQGYPAAEIQNSDEITGFVRVQPLALARGYASVPSVAPEIIPAITS
                     INIMDIQPAQTLQEEKNWIIRTISFLASAVAGEEKEIDGYIIANGCINGIKNALGWDM
                     ELDLVSNKSGEPVAVTFRSSLLSFIKPLNKTTP"
     gene            37425..>37646
                     /locus_tag="PLA17_00200"
     CDS             37425..>37646
                     /locus_tag="PLA17_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83860.1"
                     /translation="MMKRVLISAFLIVSMLLPALAQEKGKEQKLQELEQLEQQVKQAT
                     IPDTPVSAAGDTLVAEAEDLLVAAAEHTPV"
     assembly_gap    37647..37746
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37747..38707
                     /locus_tag="PLA17_00205"
     CDS             <37747..38707
                     /locus_tag="PLA17_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPK83861.1"
                     /translation="LVAEAEDLLVAAAEHTPVTTAEDTLVAAAEDTLAVIMAEDEEIA
                     VVVSDDADDTSTVRIGEIVTIKNTDDETIIRIGRKGVRVYDEGDSTEIDFVDKEYGKK
                     ERGRTGRHFYGHLGGIEIGYNNFSTATWLKNDAPIAGYLDLNTSKSINFNIVSPPASM
                     GFTRHFGLVTALGISFNNYRFEGNNSITVDEGGIISPLFPADPVRYDKSKLATIYGIL
                     PVVLEVQIPVSRGSSINLGAGVIGAVKLGSYTKVVHYNNGKQKEKNHEDFNLNMLRYG
                     VTVRAGYEMVQVYGTCYLSPMFEKGKGPELYPFEVGIAVTIND"
     gene            38901..39620
                     /gene="pepE"
                     /locus_tag="PLA17_00210"
     CDS             38901..39620
                     /gene="pepE"
                     /locus_tag="PLA17_00210"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HPK83862.1"
                     /translation="MRLLLISNSTNAGEAYLDYPKNNIRDFLGSRKVKALFIPYAAVT
                     FSYDEYEKKVKNRFNEVGHDVVSIHHFSDPVKAIMDAPAIVVGGGNTWMLLKCLRDNK
                     LIAPVRKKVMSGTPYIGWSAGSNVTCPTIMTTNDMPVTAPSSFRAFGLIPFQINPHYL
                     DANPAGHAGETREQRIEEFIEVNRDLYVAGLREGTMFLLEGKKLSLIGPRNARIFRYG
                     SEPREVKPGSDLSFLLKKNKQ"
     gene            39648..42245
                     /locus_tag="PLA17_00215"
     CDS             39648..42245
                     /locus_tag="PLA17_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPK83863.1"
                     /translation="MFRKIALIAAGVLLLLILVPGSYALLRQEHLTNSIVRKVNESVN
                     TKISYGNLHVTIFESFPNITVRFNDLLVEPSPFYDKKQLSHEDNDTLLYASSLSLTVS
                     TPSLLTSTVAVKSITARNGEVNLLTDKRGDINYEVFSEKKGDGKNLRLKNISAIDIRT
                     VYNDISTGMRMAGRIGDATLGGELFGTGIYLNTALNAEIDSINLEKITMRDIPVEAGI
                     RLRKSASSLSVVKGSLSIADLRFEMTGNVNYSSSTLNLVIEGRKINIASVISRLPDRW
                     KTVTGNFSPSGIIDLKCSITGPYGDAGKPHIDLTYGLERGRMSLSPSGFRVNNLEFSG
                     ALTNGDHNSAETFRFTVDKLTARYGSASVKGSFMMNNLRRPHITLNLNGDLDFDDLDK
                     IFRSGYIKDQTGLLSGSIRLSGMLPDSILPATSLAFLNPDISLFFREFGATLAGSRLA
                     IKDVTGSLSVKNDLTADNLSFTFLDQRFTINTVMKNFFSWMAGGGQSLDISGDIHVDR
                     LVTMALLPAKREQPDSTGKPVNLFPAAVSANILLKADSLIFNDFRAANFSSRLTYRPY
                     VITFDEVNAIGLDGVLDGELMLGRQQDGGYITKASLDVKNVDINQAFTSFNNFGQNFI
                     VSDNLRGRLTGKVTMLSPLDSNYRFIRKAIVAESHLVINDGKLISFPPAESLSAYLDL
                     DELKNISFSKMENDIFINYGTVSIPKMLINSSAVNFTVYGTHGFEGDYIYHVRLLLSE
                     VMSRKARERNRNVSSFGQVKVDGSGKATVPLKIECVNGKTSVGYDFGQAHDNIKNDIA
                     AEKQALKGILNEEYGWYSSDTTRTTPVESKPKFSITWEEGKEQPDVAEPQKEEVKESP
                     FKILLKKKR"
     gene            42249..43394
                     /locus_tag="PLA17_00220"
     CDS             42249..43394
                     /locus_tag="PLA17_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK83864.1"
                     /translation="MNIYRKKLLWKVLLLIFAVVIGLGSLIYTHDLVDSLKEEERKKA
                     EQWADATRRLVTSDDFEFLFSIIEDNTTVPVILIDEEESIISQRNLPAAFATDTLAMV
                     RELKRMAKRNDPISIELPNGIVNHLYYRESTLLRQLRVYPYVQLAVILLFIGVAYVAF
                     SASRVAEQNQVWVGLSRETAHQLGTPASSLSAWSEIISSRYPGTDIAEELPADVDRLS
                     RVAERFSLIGSKPKLMPDNLPALAEATASYLRKRIASTIDLKVADETTGCMAVPFNAV
                     LLGWVIENLVKNSADAMKGTGRITVRLSENSTEAFIDVEDTGKGIPRKDFKTIFKPGY
                     TTRERGWGLGLSLSKRIVEEYHKGKVFVLRSEPGHGSCMRIILKKQV"
     gene            43407..44669
                     /locus_tag="PLA17_00225"
     CDS             43407..44669
                     /locus_tag="PLA17_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPK83865.1"
                     /translation="MINRNDIEIMAPAGSWEALRAAIAGGADSVYFGLGKLNMRARSS
                     HNFTNDDLPAIVALCREHGLKSYLTLNIVVYDEELSEMKEVLAAAAAAGITAVIATDQ
                     AAILAARETGIEVHLSTQLNISNAGSLKFYSQWADVAVLSRELNLQQVKKIHNAIISD
                     DLRGPGGGLMKLEMFVHGALCMAISGKCYLSLHEHNASANRGNCYQTCRRGYTVSDRE
                     TGYELDIDNQYIMSPKDLCTISFLDQIIDAGARVLKIEGRARPPEYVKIVTRCYSEAA
                     TAVADGTFGQEKVEGWMRQLVTVFNRGFWDGYYLGQKLGEWSTSYGSAATKRKIYLGK
                     VTNYYAKPGVAEIKLETGDLSTGDELLITGPSTGIVELTVKELRDALEKDSAKVMKGE
                     LCAVKTDGIVRRSDKVFKWVDASSLKNE"
     gene            complement(44672..45709)
                     /gene="mnmH"
                     /locus_tag="PLA17_00230"
     CDS             complement(44672..45709)
                     /gene="mnmH"
                     /locus_tag="PLA17_00230"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HPK83866.1"
                     /translation="MAFTLNTLPADEFLQLRASLPLADVRSPGEYVSGHIPGAVNIPL
                     FDDAQRAEVGTVYRREGNARAVVRGIDLTAPGMSGKLKSGLKLAAGGKLLVYCWRGGM
                     RSEAMAWLFSLGGIEPLVLTGGYKAYRNHILGDLGRERNYIILGGLTGSGKTGILRRM
                     KSAGEQVTDLEGLASHRGSAFGALGQALQPSSEHFANMLYDDLNAHRADSPVWLEDES
                     RNIGTVFMPDPFFDRIQSAPVIALMMSIETRMPRLVREYTSFPVEEIMASVVKISKRL
                     GGDRARETLDALKRGDYPTAIRVILEYYDKSYHYGLSKRPQGQVIYINTETDDEDVNT
                     ALVMQAAEGLR"
     gene            complement(45712..48537)
                     /locus_tag="PLA17_00235"
     CDS             complement(45712..48537)
                     /locus_tag="PLA17_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK83867.1"
                     /translation="MKRASLAAYIAFILLMLVTSLFYISLYRNQIEYSNKLLDRQVMI
                     AGSDIDNTSMYIISDLTEIGFSDDITLFFADPGVNERVREKMKLYYSKYSDIIVGLMF
                     YNTNGDVYTLFKDEERNSWLDGSYRAQTVPTMHTMDKLEPDRDRYKYYLPVLHEGEVL
                     GNLVITIDFRRYFSGIFAKYNLEKYQWQWVISDSGTIIFDNGGDKRSYTNVDRITRAL
                     EQGNSGRLTHAMRTEGENGKREEILSSYYPVNILGLDFSLVFSAYMEFFQKYIVRNSV
                     ILGLLTMLTILFIILLFRRFSRKQSKALEMKTTSEAGLLSIIDRMPVGFIIHNKGMEI
                     LRANRQAAGLFAFEDETAITGKLMPDLSGNKYAMDIAGKLGEGKVIRLPSPGGEKIIF
                     LNTVPVTYNGEECTLEALIDITSLELARRHEADANIAKSELLARMSFEIRTPLNGILG
                     MTEMLNRSDLPEESKEVARLLRRSAELLLTIINDIFDVSKVETGKMILDEIPFRLRDE
                     VAYCINMIRRRNPEIPVKIESEIEQAVPENIIGDPYRLRQVIINLMTNSVAGTQSGLI
                     KLSCRVRKSEGNSFMLEFTLTDSGNTYTRAEIKRLFGDYITTISERSDWAEDLKLGPV
                     LARQLTELMGGELTAESPAMKDASGQEKGLRVIFTIKVHLNEKISKNIDLGMYADVND
                     IRTLVITGQQGRDDDFLSIMHRVGLPVSVTSFQKHTASQIRSGLNSGTGRNILLVLFD
                     EPENDGFEAAKVLMDEGLTSEHIVLMFTSRDPKGHWARCVDLGIDHLLVKPFAPEDLM
                     NVLREHFPALKKPVARLDSDKTDIPVVLVVDDNYLNRKVVGSLLRVLGVSAEFASGGA
                     EAIDMAGVKSYDLILMDLIMPETDGFEATRIILSKNPSTQIIALSADNMPETRLNVEK
                     SGMKELLAKPVTVEELRRVIDRYHKPS"
     gene            complement(48565..49533)
                     /gene="rsgA"
                     /locus_tag="PLA17_00240"
     CDS             complement(48565..49533)
                     /gene="rsgA"
                     /locus_tag="PLA17_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPK83868.1"
                     /translation="MPAESWCHFFYFCCKIRLVKRGIVIKSTGKRYRVADEEGNIYEC
                     LLRGRFRVSGARTTNPVAVGDHVRFDADEGVITELMPRCNYIIRRATNLSRESQIIAA
                     NIDQAFLIITVKMPVTLVEFIDRFLATAEAYRIPAVILINKTDLYGEEEMARAYQIRD
                     VYEKIGYRVMMISATEDKSLARVEDALRNRITLFSGNSGVGKSTLLNRLNPKLSLTTG
                     SISDYHEQGKHITTYPEMHPVADGGYVIDTPGIKAFGVIDFDRNEIYHFFPEIFRISS
                     GCRFYNCLHLDEPDCMVREAVARDDIELWRYRSYISMMLEDNEKYR"
     gene            complement(49566..50771)
                     /locus_tag="PLA17_00245"
     CDS             complement(49566..50771)
                     /locus_tag="PLA17_00245"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPK83869.1"
                     /translation="MANQKKTKGVIGILTGGGDVPGLNPAIRAVTIRANREGYKVIGL
                     RRGWGGILEIVRDPKADNSLCYQVLTDDMVNRTGRTGGTFLHTSRTRPSHVAKNYVPD
                     HLKDTYKDEINDMTPEVLKNLEWLGIDYLIPIGGDDTLSYAVHLYKQGVKVVAIPKTM
                     DNDVPGTDYCIGFSTCVTRTIQMTNSLRTSAGSHERIMVLEVFGRYAGFTAMLPTMAG
                     AANRCVIPEYKFDIEQLTRLLVEDRNTNPSRYSIVLVSEGTLYKGGEMIFQDHEHDAY
                     GHAKLGGIGDLISAAIKEHSAKFNNGKTINIINQKLGYMVRGGDPDAVDSIVPMAFGN
                     LALDLIMKGIHGRVVVLKNGRYDNVPIDVVTSSKKIVKVAEYYNTERLRPYYSNFEMK
                     PFFLMASEY"
     gene            complement(50941..52896)
                     /locus_tag="PLA17_00250"
     CDS             complement(50941..52896)
                     /locus_tag="PLA17_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPK83870.1"
                     /translation="MKKITGAFILFLFVSLIFPVEAQQPVLPDSSLYTVKGPRHGVSF
                     FPRVYHPEVEYTPGDTLTFDRYHTYGVMCHWMNRWAEDYPEIVTLYETGRSYEGRPVM
                     QMTITSSKNGSDTDKPGAFFEGNRHSGEITSAESVLWLARHLIEGYGNDPSVTDLLDH
                     FTVYLRPVNNPDGHELYLNTAQSNRSTVRPDDNDGDGLFDEDAPEDLDGDGIILTLRW
                     PDRKNGTLIPDPDDPSGRIMKRVPRGEGIYATSSEGIDNDGDGRINEDGIGGLDLHRN
                     YPENWRPEREQTGRGFTQRGAGAYPLSEPETRAVFTFLMTHPNIYVVNSMDTTVPMHL
                     RAPSTSPSEERMYPEDLKWYRYFDDLGRNITGYERAGDVYDDYGYGSPLFGHGPDFGY
                     WYYGAIWYGDELWNGGRHKDYDGDGDKDQIDMLAWDDEENNGEGFHEWKPVSHPVYDS
                     VEAGGFHPKFFSQNPPPAHLEKWASNQALFNKELISHLPRLEWEEYSVKRIKKYRADS
                     ADYKLTIRFRNAGKLPTALRQAHLVKMVRPDEVRVKFAADTTGTPRSRVIEPAPPVSG
                     RGSMMMFEEWNRRPDYITATVGYTPGGATAEANFIIRVYGETEIRGTATVVTTRAGIL
                     PEKEFIIGSITSQESSRVPSAQARSTD"
     gene            complement(52953..53606)
                     /locus_tag="PLA17_00255"
     CDS             complement(52953..53606)
                     /locus_tag="PLA17_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPK83871.1"
                     /translation="MRNLVFTNLAEDDVRRVCESKEEFSYLKGEIIHSEGESIKYFKY
                     LKNGLVKLFRVTSEGNEQIITITRPFEFVSNTNAFHEERYSYSLSALEDSIVCCVHIE
                     FIKDLILRNGKFALNLISSYARTAEGIISLGLEIRKRNLAGRTAFVLLYFSNEIYHSR
                     IFELPVSRKEIADLISMSSANVIRTFSEFRRDGIIRANGRNIEIIDINKLEVISRRG"
     gene            53753..54541
                     /locus_tag="PLA17_00260"
     CDS             53753..54541
                     /locus_tag="PLA17_00260"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPK83872.1"
                     /translation="MIYGTDYHMHTLYSDGKREPEAYIEAAIRAGLREVGFSEHLNPL
                     GRDRDWCIDHDRLPAYAEHIMRLKTEYKEQIAVRLGLEIDYLPGTEKDTERIIDSFPF
                     DFIIGSVHYLGDETVDLGPQFYIGRDVDNIYENYFNLVCEAASTGFFDIMGHPDLVRI
                     FRFYPQADISLLYSMMASSFSIHDVAFELNTNGRNKPLRDFYPDKQYLHLFAQQGVAV
                     CVNSDAHFPERVGQYFREAYDLLKRAGFREMAAFSGRERFMIPF"
     gene            complement(54594..55160)
                     /locus_tag="PLA17_00265"
     CDS             complement(54594..55160)
                     /locus_tag="PLA17_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPK83873.1"
                     /translation="MDLITITAIALGLSVDSFAVSLSCGVIESKIHFRNAVRIAFILA
                     LFQGGLTVLGFFLGSAVSDYISRYDHWVAFILLMVLGIRMVIGGLKGSSDRQPIDINR
                     LPVVLTMAVGTSIDAFAVGISLAFLKVRIWTAGLIIGAVTFLASMTAIRIGKYAGTRL
                     GSKVEIIGGIILAVIGIRILAGHLMSGG"
     gene            55222..55674
                     /gene="dtd"
                     /locus_tag="PLA17_00270"
     CDS             55222..55674
                     /gene="dtd"
                     /locus_tag="PLA17_00270"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPK83874.1"
                     /translation="MKIVVQRVKRASVTIDGKVHSSIGKGLMVLAGFEDADSAEDVNW
                     MAAKVVNLRLFDDSNGVMNLSAVETGNEIMIVSQFTLHASTRKGNRPSYIRAARPEKA
                     IPLYEEFISRTTGLLGRIPATGVFGAMMDIELVNDGPVTIIIDSKNRE"
     gene            55671..57047
                     /locus_tag="PLA17_00275"
     CDS             55671..57047
                     /locus_tag="PLA17_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK83875.1"
                     /translation="MTEKELKRSVLTVTVFASFLTPFMGSAVNLALPSIGKELSLNAV
                     TLNWIISSYMLTTSILLLPAGRAGDILGRRKVFTAGMAVFTLSMILLTFTPGIRWLIG
                     SRILQGIGGAMMFGTNMAILTSVFPPGERGRAMGINVTAVYIGLASGPFLGGLLTRYP
                     GWRSIFALLVPMGIISLILIRTKMKREWAEAKGESFDWTGSVIYGISMAALMYGFSRL
                     PSAEGWAITATGVVLMPLFIMWEKKVVHPLFDVTLISHNRIFAFSSLAALIHYAATSA
                     IGFFLSLFLQYIKDLGPREAGFVLMSWPLTMALFSPAAGKLSDKYNPGMLASTGMGIT
                     SAGLIMLCFLNEQTPVTSIVAILVIMGAGFSLFSSPNSNAIMSSVEKRQLGNASGMLG
                     TMRNVGQTLSMAIALMLLTIYMGQEKINPGNYPQLMNSMKTGFIIFSILCFAGIFASL
                     ARNKSLRK"
     gene            57143..58015
                     /gene="deoC"
                     /locus_tag="PLA17_00280"
     CDS             57143..58015
                     /gene="deoC"
                     /locus_tag="PLA17_00280"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPK83876.1"
                     /translation="MSKLYKKLFINDYSPGEMGECIAEAKRMPGAEERALILKDIFSF
                     IDLTSLNTSDSDGTIRSFAEKTAGFSYHFNDISNVAALCVYPNFVKVAKEGLGGCGVR
                     LAVVAAAFPTAQTFKEVKILETSMAVSMGADEIDVVIPVGTFLKGDIDTVIDELKAIR
                     EAAGNSTLKVILETGLLKDERNIYRASIIAMESGADFVKTSTGKSSVSATPEAAWVIC
                     RAIRDYYNETGIMIGFKPAGGIVTPNDAFIYYFIVKNMLGAKWLTPEYFRIGASRLAN
                     HILSEFYPGKPAYY"
     gene            58025..58882
                     /locus_tag="PLA17_00285"
     CDS             58025..58882
                     /locus_tag="PLA17_00285"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HPK83877.1"
                     /translation="MASGPGDTISVAADTLSLPVQQTEFSIGQIIPLVSPAEDIAVKD
                     ERVAGLTADYISGLKRVDGIPRASGPVNSDMSFILLSLSFLIITLLTLFGRRTVITGL
                     SSISFRRNEENIPVGTSEVFTWPPIFRNIFSVLNISLFASTSLLVTGLVEDTLFNGST
                     GLTAIIAGSFLASLFLRHLSCIVIAGLTGFKSLFREYMNVIYNSWFACSILLFILNGL
                     IHFTAVQNPLPFIIAGLIIIAIFLLIRVLVLLSIFIDRHVSIFYYILYLCALEVLPVM
                     VILKLLGVF"
     gene            58924..59673
                     /locus_tag="PLA17_00290"
     CDS             58924..59673
                     /locus_tag="PLA17_00290"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPK83878.1"
                     /translation="MKIRKVLVSQPEPTNPKSPYFELAKKYNLKIDFKQFIQVEGVSA
                     KDFRQQKINLTSFSAIIFTSKIAIDHYFRICEELRITVPDTMKYFCITENVAFYLQKY
                     IVYRKRKVFHGKACFEDLIDVIIKHKEEKFFVPLSDTHKSDIPELLAKNKIKHTIGTM
                     YRTISRDFDCKELDYDLLVFFSPSGIKSLKENFPTFVQGDVKIAAFGPTTAEAVTNEG
                     FRLDLQAPNPKAPSMTMAIDNFLKEYNKNCK"
     gene            59739..60128
                     /locus_tag="PLA17_00295"
     CDS             59739..60128
                     /locus_tag="PLA17_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPK83879.1"
                     /translation="MKKQKASFSKSERLCGVKAVSELFAEGKSLILPSLRVICRFTPA
                     VEGIHPVRVLITVPKRHFKKAVDRNLMRRRIREAWRKNKEPLVTLMKQAGRHADIALI
                     WTDTSVRPYEFAEKCVTDAVSRVSGLK"
     gene            60151..61824
                     /locus_tag="PLA17_00300"
     CDS             60151..61824
                     /locus_tag="PLA17_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPK83880.1"
                     /translation="MNSKKIKKRVLVPAITVTAVITLGFLLGGQEKKDFRLTKNLDIY
                     ISLFRELNAFYVDEIDPEKMVTRSINSMLATLDPYTVYYPEEETDDLDFMTTGKYGGF
                     GSLIRKSGDYILVTNVYKGFPADKSGIRPGDLMISIDGTSLKGVSSEKASDMLKGEPG
                     TEAEIVIRRNGQEITKKLRRERIAISAVPYYGMVDENTGYIRFTNFTQNCIAEVREAL
                     IDLKDKQGAEDLILDLRSNPGGLVNEAVEIVNLFVRPGQEVVSTRGRAKQYDAVFRTN
                     RTPVAPDMPVVVLINRGSASASEIVAGALQDLDRGVIVGERSYGKGLVQVARPLSYKA
                     QVKMTTAKYYIPSGRCIQAVDFSNRNEDGSVGTIPDSLIKTFSTRSGRPVKDGGGIIP
                     DVTVPSDMLNRFATELYMQNMIFDFATQYYWSNPRPPVLDSLKITENDLDQFTNFLAE
                     KKFSYRTDSESILEELTKTAREEGLYDSNREAMEKLKSGLSHTLEREMTLYRTDVTEL
                     IESELAGRYFYDWGMVEYSVTRDNQIKKAIEIAGNREQYASLLKAPTGV"
     gene            61900..62088
                     /locus_tag="PLA17_00305"
     CDS             61900..62088
                     /locus_tag="PLA17_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83881.1"
                     /translation="MSSPATEERNPATESRGPGTEERCLIIEERSPATDGRSLIRDEE
                     RSPATEERRLTRDGNLSQ"
     gene            complement(62082..62483)
                     /locus_tag="PLA17_00310"
     CDS             complement(62082..62483)
                     /locus_tag="PLA17_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPK83882.1"
                     /translation="MKNSCLIKGPITPVLISELMASGNDDAGAGAVSIFIGRVRDDET
                     DGKRVTAIEYSAYEEMAVKEVEEVITTVRSAFSDVRSVFIVHSLGVVMAGEASLFIMV
                     TSGHRDHATRACRHTLEMVKERLPVWKKELY"
     gene            complement(62501..62737)
                     /locus_tag="PLA17_00315"
     CDS             complement(62501..62737)
                     /locus_tag="PLA17_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPK83883.1"
                     /translation="MKAEVRVLYFGAAQEATGKSEETIMAADTASLRNQLISRYPALK
                     QIPFRMALNRTLLKGDSGLQKSDTIAIMPPFQGG"
     gene            complement(62740..65586)
                     /gene="polA"
                     /locus_tag="PLA17_00320"
     CDS             complement(62740..65586)
                     /gene="polA"
                     /locus_tag="PLA17_00320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPK83884.1"
                     /translation="MKETDNDRRLFLLDAYALIFRSYYAFIRNPRVTSGGMNTSAIFG
                     FLLTLEDLLSKQKPSHIAVVFDPPGPTFRNEIYPAYKANRDPTPEDIRIAVPYIKRLL
                     EAFRIPVIEVPGYEADDVIGTLARKAAEQGYRAFMMTPDKDFAQLVTDNVVMYRPGRS
                     GGEAEVWGRDEIVREFGVPPEHITDLLGLMGDTSDNIPGAPGIGPKTACKLINEHGAV
                     ESLLENTSALQGRQKDIIEQNREQILLSKQLATIEQNVPVELRHTIFHRQEADTEALR
                     KLYEELEFRTMAAKLPAPGVKQAAPADLLSQPAGDSAAQEESPATAMPKPGESQGSLF
                     GDLLSPAGKTTAADINSVPHRYTLIETEKEAAMLASELSKLSDFCFDTETNSLDPLNA
                     QLVSIAFSWEKGSGTMLWLPPEKDAALKLLEPLRPVMENEKIRKTGQNLKFDMQVLAG
                     YGISVRGPLFDTMIAHYLLEPDKRHNMDLLAEQYLGYKPVHIEELIGERGPRQKTMRE
                     IDRERVKEYAAEDADVTWQLMKLFEPMLIQNELDALAADVEMPLIRVLTAMERTGVLL
                     DESVLTNYAVTLRENLIRLEQEIYTYAGQEFNISSPKQLGDILFVRLRLDANARLTKT
                     KQYRTDEEVLQRLTGRHPIVERVLDYRGLKKLLSTYVEALPQLVDPVTGRIHTTYNQA
                     VTSTGRLSSTNPNLQNIPVRDSEGREIRKAFIPASGKIFLSADYSQIELRLMAHLSGD
                     SAMISDFLSGQDIHAATASKIFGVPVADVTREMRARAKTANFGIIYGISAFGLSERLT
                     IGRKEARELIDGYFNSYPGVRAYMDESIRRARELGYVTTMFGRRRYLPDIHSRNQVVR
                     GNAERNAINAPIQGSAADIIKIAMVRIASRLEREMPGAKMILQVHDELIFEVEESQVK
                     RLADIVTEEMKGAAELSVPLDVDTGTGYNWLEAH"
     gene            complement(65583..66032)
                     /locus_tag="PLA17_00325"
     CDS             complement(65583..66032)
                     /locus_tag="PLA17_00325"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPK83885.1"
                     /translation="MALIRVTKEFRFEMAHALWNYDGPCRNVHGHSYILYVTVAGEPV
                     NNPDHPGNGMVIDFGDLRKIVHESIVDRFDHALMVSGLAPATTVETYRQHFGNVIVSP
                     YQPTCENLVADIAATLGSRMPEGTYLHSIRLFETATSCAEWHASDNR"
     gene            66175..67812
                     /locus_tag="PLA17_00330"
     CDS             66175..67812
                     /locus_tag="PLA17_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPK83886.1"
                     /translation="MTNNEILTIPSLMGESFRKHGNNQAMGFVGEDAITYNQLRERTR
                     SVMRLLEDLRIEQGDKVAILSTGMANWGVAYLAITSMGAVAVPILPDFTPLEIENILN
                     HSGTKALFVSGNLAHKISGYGPETLETVIAIDDFRITSGNDQISFVAGSEPSGTYNVA
                     EEDLAVIIYTSGTTGKSKGVMLTHRNICFTARQIMGIQYVVESDVFLSVLPLSHTYEQ
                     TLGLVVPLFNGAVIWYLRKPPTPSVLLPALQQIRPTTMLTVPLIMEKIYFNKVVPTFQ
                     KNKVLAFLYGTRLFRPLFNRIAGKKIYRAFGGRLRFFGIGGAKLNPTVEQFLLDARFP
                     YAIGYGLTETSPLLAGTKVGTCRLGSTGPAMPGITLKINDPDPVTGEGEIWAKGDNVM
                     KGYYREPAITAEVLTPDGWFKTGDLGVVDKNNFLSIKGRLKNMIVGSSGENIYPEEIE
                     SVINDFRHVIESLVIQKKGKLVAMVYLNIEELQAKYAQMKKDFNKQAEIINEYLEELR
                     RYVNARVNRYSQLQLVVYQPEPFQKTATLKIKRFLYY"
     gene            67909..68520
                     /locus_tag="PLA17_00335"
     CDS             67909..68520
                     /locus_tag="PLA17_00335"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HPK83887.1"
                     /translation="MSNRTKALLLIFTILIADQIVKIIVKTNMVIGEEIPVLGSWFRI
                     HYLENNGMAFGLEWGGKAGKIALSLFRMAAIAGIAWYLDSIIKKNAHTGLILSISAII
                     AGAAGNLIDSAFYGMIFSESWHTPAVLFPEGGGYSSFMLGRVVDMLYFPIIDTHWPDW
                     SPFRPGQTFIFFRPVFNIADASITTGVLAILVFQKRFFGENKS"
     gene            68615..69589
                     /locus_tag="PLA17_00340"
     CDS             68615..69589
                     /locus_tag="PLA17_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPK83888.1"
                     /translation="MASLSDIKRPVETEMREFESYFRRTLQSDIPFFHVILDNLLRRK
                     GKQMRPLLVFLTARLNGTINETTYVAATFIELLHTATLLHDDVVDEASERRGFPSINV
                     MYNSKIAVLAGDYLLAQGLLISVEKQSFNMLGIVSEAVREMVKGELVQLQKTRKLDIS
                     EDDYFRIIKMKTAALISACTACGTSSVTDDPETIARMKEMGQNIGIAFQIKDDLLDYT
                     GNGLTGKRDGNDIKEKKITLPLIYALREAGPVERRHIFRLLRKKKKSSKEIREVIGFV
                     KKHGGLEYAERVMKEYSDRAIRILEKYSDSDTKSALTEFIIFSTLRRK"
     gene            complement(70004..71797)
                     /gene="uvrC"
                     /locus_tag="PLA17_00345"
     CDS             complement(70004..71797)
                     /gene="uvrC"
                     /locus_tag="PLA17_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPK83889.1"
                     /translation="MSAVEALKSTVSALPDKPGVYQFIDASGTILYVGKARSLRKRVG
                     SYFTGNQSGKTRVMLSRAADLRHIVVDNESEALLLENSLIKKHQPRYNILLKDDKTYP
                     WICVKNEHFPRVFMTRRLVADGSAYFGPYTSVPAVKTLLELVRHLFHVRTCSLPLNTR
                     SVSEGKFKTCLEYHIGNCEAPCIGLISEEKYNLQTDRIKEIIKGDLSGVTAYLESLMR
                     QYAKDLKFEEAQKIKEKLELLSKYRSKSVVVNTSVRNVDVFGCSAEEGSIYISYIKVV
                     SGAIVQSWSVELKMRMEEEKESLLSTAVTEIRQRVSSDSQEVIVPFMPDIKLDKVKYT
                     VPQRGDRYKLLELANRNAVFFKLERQKKRADRSKEKQTEKNLEKMKKDLHMPELPLHI
                     ECFDNSNIQGESAVAACVVFRNGRPSNREYRHFNIKTVTGPDDFASMEEVIYRRYRRM
                     LDEQKPLPQLVVIDGGKGQLSSAVKSLDALGIRGRLTVIGIAKKLEEIYFPGDTVPLY
                     LDKNSITLKIIQHLRNEAHRFGINFHRGKRSSVMNRSILDEIPGIGEKTKERLLKKFG
                     SVKKLSEATVEELEKIVGAKKASLLAAALRN"
     gene            complement(71989..73635)
                     /gene="ade"
                     /locus_tag="PLA17_00350"
     CDS             complement(71989..73635)
                     /gene="ade"
                     /locus_tag="PLA17_00350"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPK83890.1"
                     /translation="MNIAGKIIDVHKKRIYPGIITIKAGKIESVTETEESPDHFIMPG
                     LADAHVHIESSMLVPSHFAEAAVKHGTVAVVTDPHEIANVLGKEGVDFMMDNAEDVPV
                     KIIFGAPSCVPATSLESAGAKIGSAEIKELIEEGKVKFLAEMMNFPGVIYNDAEVHLK
                     LEAARNAGIPIDGHAPGLTGEELKKYISAGITTDHECSSLEEALEKISGGMKILIREG
                     SAARNLEVLAPLISYYPERVMLCTDDFHPEALLRGHINKLVARLISMDYNIFDVIRVA
                     SLNTYEHYRINAGTLRPGDPADFIIVDDPKTMNVLQTWIDGKCVFDGEKVLFSPGEII
                     KINKFKCTRLLPDEIRVINEGRKINLIVARNGEITTESDIVQAGEEEFVNPRPEDDIL
                     KIVVKERYNDKPPAMAFIRGFGMKQGAMATSVAHDSHNIIAVGTNDRDIVNAINMIVD
                     AGGGMAVASEEGTEILELPVAGIMSDLPVTEMASRYEKLSERVRSLGSRLDAPFMTLS
                     FMALLVIPKMKMSHKGLFNVLTFKHMPLFVSDPYSKYRIN"
     gene            complement(73676..75550)
                     /gene="mnmG"
                     /locus_tag="PLA17_00355"
     CDS             complement(73676..75550)
                     /gene="mnmG"
                     /locus_tag="PLA17_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPK83891.1"
                     /translation="MILHYDIIVVGGGHAGCEAAHIAAVMGAKTLLVTMDMTKLARMS
                     CNPAMGGIAKGQIIREIDALGGCSAIVTDRSMIQYRMLNRSKGPAMWSPRAQCDRMLF
                     SMEWRKILEKTDNLAIWQEVADSIIIEDGNIRGITTTLGTELHADAVILTNGTFLNGL
                     MHVGFNTMSGGRSGDPDSRGLSEQLRELGFRVERLKTGTSARVDGRSVNFSAMVEQKG
                     DNEGRCFSYLHNRVDIKNQKSCYITHTNEIAHGIIREGLKYSPLYTGRIKGSGPRYCP
                     SIEDKIVTFAGKETHQLFLEPEGWETNEYYINGFASSLKLETQLSALRKIRGLENVVI
                     YRPGYAIEYDFFQPTQLKLSLETKRIGGLFFAGQINGTTGYEEAAAQGLMAGINAVRR
                     MQGKEPFILNRDEAYIGVLIDDLVTKGIDEPYRMFTSRAEYRILLRQDNADERLTPKA
                     AELGTASKDRIRRVEEKYRMVDELVTFLRNHTVSPEEINTFLKEKNTTEINQKIKAIG
                     IATRPQIYLEELLDTMHVEERLSYGKSERKSEIIQAAEIKIKYSGYLEREKGVAEKIK
                     KFERLKIPEEIDYDELISISTEGREKLSRIKPATIGQASRISGVSSSDVSILIMYLGR
                     "
     gene            complement(75547..75969)
                     /gene="ybeY"
                     /locus_tag="PLA17_00360"
     CDS             complement(75547..75969)
                     /gene="ybeY"
                     /locus_tag="PLA17_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPK83892.1"
                     /translation="MRVSFHYDIEKFRLRESRIIKKILNRIVSDARLRTGIIDIIMTT
                     DEKLHGINKEFLGHDYYTDVIAFNYNKGNTVNGEIYISVDRVRQNAKKFNVPFRSEIR
                     RVIFHGLLHLCGYDDREDEGKKKMSEMEDIYLALSYAE"
     gene            complement(75974..76366)
                     /locus_tag="PLA17_00365"
     CDS             complement(75974..76366)
                     /locus_tag="PLA17_00365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK83893.1"
                     /translation="MESITINSNIENLRAVEAQVDKLSKKLGISDEVYGKILISTVEA
                     VNNAILHGNKGIETKTVKVDFIADGTVFEVTVTDQGEGFDYNHLPDPTDPANIENLHG
                     RGVFIMRSLADNIEYNPSGNQVRMKFKY"
     gene            complement(76426..77523)
                     /locus_tag="PLA17_00370"
     CDS             complement(76426..77523)
                     /locus_tag="PLA17_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPK83894.1"
                     /translation="MRKKNLQEIEYVDKLLKTTESQKNESLNELRVIGRKLNLREKLI
                     AEFGQEINLLEYRIDLNALAIEMLEQDLNDHKKDYERSILSAYKATKGMPALAFILSA
                     TDFNQGYKRLKYIQQVARYRRNQAETIETLYSQLQATKERLEKDRKNVNELKNKEVRQ
                     RQILKQEQGRKEKLVSSLSRKEKQLNQELNEKRRIARQIEKEIEKLIEAERKKGGAAP
                     MSAEMKVISESFGENRGRIPWPVEQGIITSHFGSHQHPVLKNVIEDNIGIEITANGPT
                     KAKSVFKGMVGGVFAISGANMAIIIRHGKYLTVYQNLVNVVVKEGDAVLTGQIIGDVF
                     VDNGDGGKAKLKFMVYEEKKKLDPEQWLTKK"
     gene            complement(77604..78368)
                     /locus_tag="PLA17_00375"
     CDS             complement(77604..78368)
                     /locus_tag="PLA17_00375"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPK83895.1"
                     /translation="MPEAVKYAVVISLILLMTGCAAMKKRRTTVKDELAADVSYSAVM
                     RGVTSNNITGRGFEIRKGSIELEGTEVEGKFGLLARLNSKGDFYGSVRGPLGIELVRI
                     LMVGDDIAVIDRFNRKIYIGKKEMVLRKNGMPSDFMTIIFGDIPEEDDVEMISADRNE
                     IVVSSGNEDFNRLITICLDYMKVCNQRINANVSDNEIYMQFGKFSVSGGKKYPSEIMM
                     EEREKMFHVKLSIDDLIYGYDSDIEFRLPSYKKESF"
     gene            complement(78353..80068)
                     /locus_tag="PLA17_00380"
     CDS             complement(78353..80068)
                     /locus_tag="PLA17_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK83896.1"
                     /translation="MRSTIILVVILLAAAGCSEKVISPVMKGDRETRSDNRYDYILSE
                     ALRQKYVGELSEAASLFEKCIELDKYRSVPYYELAQMYSAAGMRNKSLEYASTAARLE
                     PGNYWYQLACGSLFTQYEMKDSALVYFLRAIKADTTAVEVKGILAGIYSERGETAKAD
                     SLFKILEEDGVLTPDMSLMMIYDLISMNKVDEAAERAIKLLELNPEETRYRALLADIY
                     YENGLKEKSDSIYAAIIEEDPDNIESQFLYLMNLVNRKEYTGISGFLRNIFRSEVVER
                     EGKIAVARRMAGDSAYMKENAESMIESLLLLEEQYPDDEEVISLRPAVYEATDKIDLA
                     IQRYEEIIKSIRQGFWFKERLMILYARKNEYSKLYELAGDYSRDNNRSLIGKIYYALA
                     AMELGEYSVAGNELKKAMILAGNNNELKVQVLSLEGDLHYRKKDFDASYACYEEALKL
                     SPDDPLLLNNYAYFLAEGDKDLKKALKMIMKVIETESGNATYVDTYAWVLYKMGKYRD
                     AHREMVRVFELSSDRDPELLEHMGFIKKAVGRCEEAVVYWKEALKEDSKKAYLEEEII
                     KCQKQ"
     gene            complement(80065..80499)
                     /gene="dut"
                     /locus_tag="PLA17_00385"
     CDS             complement(80065..80499)
                     /gene="dut"
                     /locus_tag="PLA17_00385"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPK83897.1"
                     /translation="MKIRIVNKSPHPLPAYETEHSAGMDLRAFTGGPVTIRPMERKLI
                     PTGLYIELPVGFEAQVRPRSGLAIRHGITVLNSPGTIDADYRGEIGVILINTSEIDYT
                     INDGDRIAQMIISQYVKVKLKKAESIEMTVRGEGGFGHSGEK"
     gene            complement(80496..81968)
                     /locus_tag="PLA17_00390"
     CDS             complement(80496..81968)
                     /locus_tag="PLA17_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPK83898.1"
                     /translation="MNDISKLAGQTVIYGFGTVVPRFLNYALLTPFYTRILGLEQYGV
                     VTELYAWMVLALVILTYGMETTYFRFAGKKANAEVVYGTAMISLFTTSAMFIMAVSMF
                     ITPISGFLGYAENPEYIRMFAWIVAIDAFSAIPFAWLRNNNRAALFSALKIINVIVTI
                     VLAFFFLKIAPSVAEKGNRWIYKVWSPDFQVGYVFVANLAGSAITLLCLTPFIFRMKP
                     VFDRRLLRKMLAYSWPLLVGGMAGSLNEVLDKILLRRLIGGAEGLATVGLYGAGYKVG
                     VLMSLFIQMYRFAAEPFFFDKAGSKDAKETYAITMKYFVITAVILFLGINLYPEIVSI
                     IIGENFRESMNVVPIISMGYLLLGIYINQSIWYKVDDKTIYGVWITLVGVAVTVAVNL
                     VFVPLYGYIAAAWAHVACYLSMVIVSYFVGQKYYPIEYEKGRIVMYIALALAMLFISM
                     MLSGDNKIVNAVIDTMLLLVFFAVAEYRDKYFTILFKKGS"
     gene            82021..82818
                     /locus_tag="PLA17_00395"
     CDS             82021..82818
                     /locus_tag="PLA17_00395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK83899.1"
                     /translation="MKAPFRIIMTLAAAIIIAGCSAGNEKGVRAVLQTTEGEIVISLS
                     DLTPLHRDNFVKLAESGFYNGVTFHRVIKSFMIQTGDGSTRQVSSPAIEDYTIPAEIN
                     DSLFHRRGAVAAARMGDDVNPERNSSGTQFYIVQGKVYNDEELEKAIERIESSRNQYF
                     YNKALKELRSEAELAGKEINEDEIQQSASLKAQEAMEEAGAYVMPEYRRDAYKTIGGA
                     VHLDGSYTVFGEVLEGMSVVDAIAGTATDQRDRPVNDIRIIKVKILK"
     gene            82833..83876
                     /gene="pheS"
                     /locus_tag="PLA17_00400"
     CDS             82833..83876
                     /gene="pheS"
                     /locus_tag="PLA17_00400"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPK83900.1"
                     /translation="MKERISELNKEVEEFNIHNEESLEQFRITYLSKKGLISMLFEGF
                     RNVPPEAKRDTGLLLNNLKQSATNKYNEHKERLAMIEVAGPSVDLTMPAYPFAEGTRH
                     PISIVRNEIIGIFSRIGFTVSVGPEIEDDNHVFTMLNFASDHPARDMQDTFFIEKKSG
                     IESDPHDVLLRTHTSSVQVRVMENQQPPIRTISPGRVFRNEAVSARAHCIFHQIEGLY
                     VAENVSFVDLKQTLLYFATELFGKDVNIRLRPSFFPFTEPSAEMDISCNICGGKGCNV
                     CKGTGWLELLGCGMVDPNVLDACGIDSEKYTGYAFGMGIERATMLKYGVNDLRLYFEN
                     DIRFLKHFRTEIL"
     gene            83910..84623
                     /locus_tag="PLA17_00405"
     CDS             83910..84623
                     /locus_tag="PLA17_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPK83901.1"
                     /translation="MARFMKQHDYTISFCDKCAFESSSVFRFLTRDETDRLNFEKDFR
                     HYKRGEILYHEGNRISGFYCINSGIIKVFKTGIDGKEQIIRFARKGEIIAYRSVLSNE
                     PACTSAKVIEDCQVCFIPSELLIQFVKTNPSFAFEVVKLTCHELAEANSYITDIAQKT
                     VRERVAEVLLSLVRDFGLDEQKYLRILLTREELANIVGTATESVIRLLSEFKSDRLIE
                     LNGRKIKIIDQKGLEKISS"
     gene            complement(84827..85846)
                     /gene="ruvB"
                     /locus_tag="PLA17_00410"
     CDS             complement(84827..85846)
                     /gene="ruvB"
                     /locus_tag="PLA17_00410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPK83902.1"
                     /translation="MEEQFNLRKEITQAGDAELERQLRPLTFSDFRGQPRITDNLNIF
                     VTAALQRGEALDHVLLHGPPGLGKTTLASIISNELRVKIKITSGPVLDKPGDLAGLLT
                     SLDEGDVLFIDEIHRLHPVVEEYLYSAMEDYQIDIMIDKGPGARSVQLKLNPFTLIGA
                     TTRSGLLTSPLRARFGIKFHIEYYDAAVLKGIVKRSASIMNITIDEDAAAEIASRSRG
                     TPRIANALLRRVRDFAQVKGSGAIDLDITRYSLEALNIDKHGLDEMDNRILRTIIDKF
                     RGGPVGLNNVATAVGEESGTIEEVYEPFLIKEGYIKRTPRGREATEHAYRHLGIVRGR
                     EEMLF"
     gene            complement(85854..87401)
                     /locus_tag="PLA17_00415"
     CDS             complement(85854..87401)
                     /locus_tag="PLA17_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83903.1"
                     /translation="MKKIYLIMLVFIPALLAAQQNVQLQDGYQQFRYPNGNISSEGYI
                     RNGKPDGYWKSYYVTGVLKSEGRRTSFLLDSLWVFYDQAGDTIEKISYLLGKRNGYSL
                     KYKKDPVYGNYLFSSELYAGDRKEGLAKIYFPDGKIKQTIPYSDSKKDGLSREYNHDG
                     KIIALLEYNNDFLISRERINYTDASGMKQGEWLDFYPDGSRHIERNYRDDVLHGYYKE
                     YDERGRLLVTLLYESGKVTGTDIDNSAEIDIQNRYDDSGRLIYSGPFKEGIPVGIHRE
                     YNSDGTIKGARIYNDNGVLISEGIIDAEGNRTGPWKDFSPGGTLIAEGNYTTNRRTGP
                     WKFYNSSGRLEQAGAYSSGRIDGTWRWYYPEGELLREEDYYQGKRDGSYTEYTRTGEI
                     IAEGSYADGERNGLWKVNAGDNTEEGEYILGLRNGEWKSYYPGGKLRFKGNYRQGNPE
                     GHHTLYFENGRTKEDRYYRNGFRTKTWKKYNELGEIILTITYRDDIETSINGVPTGLQ
                     SSIKRIK"
     gene            complement(87403..>88105)
                     /locus_tag="PLA17_00420"
     CDS             complement(87403..>88105)
                     /locus_tag="PLA17_00420"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HPK83904.1"
                     /translation="LMAGCSLADSCKKEDFEEFINDNPGRTVITYVNTTADIKALSDI
                     ACTSSNAKQIVESLHQDEKIIFGPDRNLGNYIKELTKRDILVWDGACHVHEQFSLDAI
                     LRLKKENPGSKIIAHPECEKPVRLVADYIGSTSGMLSFVSKDPGKVFIVVTEPGILYE
                     MRKAEPEKIFIPVPPKYSMCGYNECNFMKLITLEKIYKSLKFEEPEVTVDASIIEWAA
                     VPIKRMLEISAKLGL"
BASE COUNT        22104 a        22502 c        20482 g        22917 t
ORIGIN      
        1 gtagccccac caggaatcga acctggattt caggtttagg aaacccgcgt tctatccatt
       61 gaactatagg gcctgctctt tgcaggcgca aatgtaaatt ttttttcgct tatcgcaaaa
      121 aatcagcgcc ctcctgcagg cttcttcttc acctttgact tcacgggtgg cttgacaacc
      181 ttcacagcca ctacactgca cccggtggca acaatgctcc cgccacccgg agcggcagca
      241 aacagcatag ccgtaaatcc cgcaaatgct tatgaaagtt tcttatatct gaatcttacc
      301 ggaccggcat tcccggtgcg tctcctgagg ttttcatcat attccgagaa gctgccctca
      361 aaccagacca cttttgaatc accctcgaag gcaagtatat gtgttgcaac gcggtcaagg
      421 aaccagcggt catgggagat tatcacggcg cagccggcaa aattctcaag accctcttcc
      481 agcgcacgga gcgtgttcac gtcgatgtca ttggtaggtt cgtcaagaag cagcacgttg
      541 gcaccgctct tcagtgtcag tgagaggtgg agcctgttgc gctctccgcc ggagagaacg
      601 ccgcacttct tctcctggtc agcgccggtg aagttgaaac gtgcggcata ggcccttgca
      661 ttgaccggtc tgttgccaac cagaacctca tcctggccac cggagataac ttcatatacg
      721 ctctttcccg gatcaatggc ggcatgagcc tgatcaacat aacccagggt taccgtttcg
      781 ccaatctcaa atgtgcccct gtcaatggtc tcctgaccca tgatcatccg gaagagagtg
      841 gtctttccgg cgccattggg gcctattact cccacgattc cgttgggcgg caggcagaag
      901 tcaaggtctt caaacagtat tttctcaccg aaggctttgg ccacgccccg cgctgttatc
      961 accctgtcac caagtctcgg accgtttggg atgaatatct cgagcttctc ctccttctgt
     1021 tttacatcct ggttaaggag gttctcatat gcactcagac gggctttcga cttggcatgc
     1081 cttgccttgg gcgacatacg cacccactcg agttcccgct ccagtgtctt gcgcctcttt
     1141 gtgctgccct tctcctcgag ctccagccgt ctggccttct gctctagcca tgacgagtag
     1201 ttgcctttcc atggaattcc ttcaccgcga tccagctcca ggatccatcc tgccacattg
     1261 tcaaggaagt aacggtcatg cgtgatacag atgactgtac ccttgtactg cctgaggtgg
     1321 gtctcaagcc actgcacgct ctccgcatca aggtggttgg tgggctcatc gagcagcagc
     1381 acgtcaggct ccagcagcag caggcggcag agggccacgc ggcgccgctc gcctccggag
     1441 agaatgttga caggagttga tggctcggga cagttaagtg catccatcgc tctctcgagc
     1501 ttatgctcaa gattccagcc gtcaagtgct tcgatctttt cctggagtgc cgcctgcctg
     1561 gccatcagct tctccatgac atccggattc tcatatacct caggctcacc gaagcgaaga
     1621 ttgatctcct cgtactccct gagcaccgag acaacctctg cagcaccttc ctccactatc
     1681 tcgcgtaccg tccggctttc atccatcagc gggtcctgag acagatgccc caccgtgtaa
     1741 ccgggagaaa acacaacctc tccctggtac tccttctcaa gacccgcaat aatcctgagc
     1801 agcgtggatt taccggatcc gttcaggcca ataatgccga tcttcgcccc gagaaagaat
     1861 gagagtgata tatccttgat tacctgctta tggggaggat agctcttgct gaccctgtac
     1921 atagaaaaaa taatcttctt gtcgtctgcc atggcaaaat ggttatacga caaaagtaat
     1981 cattttgtct gccctgagta aatacaacca tcaccgttga ccagggtcaa atggcaatca
     2041 tgccacccgg gaaattgttc cctgttcaga ttttcacagg tgacgtcggg cacccctttg
     2101 caaagagagg tatagcctcc ggctgaacaa ggaattacac ttgcatcaaa tccaccacaa
     2161 catcctttct aaacagggaa atggcctccg gctgaacaag gacccatata atgtagactc
     2221 ccttgagtgt acccaggggt atgttgagca ggccaatacc ccctaatacc agcgtcagga
     2281 tcctggccca gcgcttgcag tagaagagac cgatgccacc ggcaatcatt cctgcgccga
     2341 aaagcagcat cagtacaacc agcggggttg agattatagc gaggatctta acggctgtag
     2401 gatcttcagc aaactggtta atgaatccga acacgactgc aatgatcagt gcgccaaaaa
     2461 caagcaatga gccgaaaact atctgcagta tgcctgcaat attgacatgc ttctttatcc
     2521 tgttctcttc ggctgtcagc actgtggatg atgcggcatt ctcttccatg atatgaacgt
     2581 tttttgtttt cagaataatg actccaattt ataaaaaatg ctgcacatat cccgttatac
     2641 taattgctta cctttaccgt tgatttacaa atgtcagtca tgaaaaagtt actttacgct
     2701 tccgctcttc ttttaattac ctgtctgttc aattcctgcg aaattctggg tgacggatgt
     2761 caggtatgcc agacggtttc gtatgagaac gggaatgctt ttgcctgggg taccgaagct
     2821 gaatactgcg gagaggaact gataagcatc aaggcaattc ctccctcgac ggtaaatggg
     2881 gtaacaaccg tctgggagtg tcgctgatac tcctataatg ctgattttat gagtattctc
     2941 cggctcttga tttcctgttt aaaagttgtt gttaataagt tgttcctaat gttgactttt
     3001 gcggcagaaa tgattattct tgcctttcct cgccggatat ttttgttatt gagttcggat
     3061 tattaacccc gggttcactc tataaacatg cttaacatta caggcaagcg gatagcaatt
     3121 gttgaggacg atattgcatc cctgaagtat tacgagacgc tgcttaagga gaccggcgca
     3181 gagattatta cttaccgtaa cgggaagaag tttgttgagt ctgtaactac aggcagggaa
     3241 cactttgatc ttgttttcat ggattttctt gtgcctctgg taaacggcat cgaatgcacc
     3301 agggcattcc ggcaggctaa ccgctctgca ccggtgattc tcattactgc atattattct
     3361 gaacaaacca aaaacgaagc atatatagcc ggctgcacgg aatacaatct caaacctgta
     3421 ttccctgaaa agatttatca gattcttgag aagtacctga ctcccaggcc ggcctatcag
     3481 atgtatgact gacgcacaga cggcgttcaa actggtccgt atcctccctt actgctgatt
     3541 cccaactaca gatcgcctac tgattaaagg cggcatatct ggcatagagt tcattgtatc
     3601 tgccattgat ctcttcggcc agcgccattt cgccgttatc aaaacagacc cttatcgcct
     3661 ggagcatcat ctgaaggcca ttcgcaattt cagcacctgc ataataagct acagatggcc
     3721 gctggtcaag cagataagct gtccgcgcag caaaataatc agtaaactca cccgtcagtt
     3781 gcttcgcttt ttccttcatg ccgctggcaa agcaggcaga gatgatatca ccgaaataat
     3841 aatcatagtc tacatttgac acagggaaga gctcaagaca cctgtcaata acctgcactg
     3901 ccctggcagt atcaccctca gccacgaggg cctgtgcaag cctcgcatgg ttcaggcgtg
     3961 ccctgattac gagaagtgtg cggttgtgat aatagtcgat gtaaacatcc ggatcattgg
     4021 cacttaacca gctgaacctg ttcatcatgt tctcgtagag aatgtcagta tcaatgctgc
     4081 catactcaaa ccagctcttg ttgacccctt ttatcggcac cagcctgtat gccagtcctt
     4141 caagctggaa gtattcctcc agcccaaggg cgtcatccgt ctggccggtg acaaagtaga
     4201 tcggacggtc ccagtcgttg tgagccagga tatcaagcac caggagctgg ttcttcatga
     4261 tccatgaacc cttgagatta atgtcaatat agggcacaat cttgtcagca tcctccggct
     4321 ttactgtgcc attggccagc accttcgcgg aatcaaccgg gatgcggatg gtgcgggtgg
     4381 gtatatacca aaggtcctct ccgtacagct tgagttttga cacctcactt ccgctggaaa
     4441 tgaattccat cacctccttt gcagttgccg gacccttcac ccgttcaaag accgccacct
     4501 ggttattgac tccgtcatag taaagctcct tctgcaggct tacaggcagg ggatcggagg
     4561 tgtaggccct gtatttcatc tggtcaatgt accagtcagt gttgagcagc atcaggttgc
     4621 aaaccctgac atcagtgcgg actccctcca cctcctgggc gtaccacagg gggaaggtgt
     4681 cattatcgcc accggtaaag attattgcat tcggggcgca tgaattgagg tagttgaaag
     4741 ccacttccct tccaaggtat cttcccgaac ggtcatggtc atcccagttc tctgatccca
     4801 tgagggcggg cacagcagcg aagcagagca cggtggcaat gatggctgaa ggcgtctcac
     4861 cggctgcctt cctgaacagc tcaaagagag ccagaacacc caggccgatc catatggtaa
     4921 aggcatagaa tgatgccgca taggcataat cgcgttcacg tggctggttc gggtactggt
     4981 tgaggtatat gacaatagct atacctgtca ttacaaacag cagaagcact atccaccagt
     5041 tgcggatatc acggttgaag tgccacagga aacctgccag cccgagaagc aggggcaaca
     5101 ggtaatatgt gttgcgcgac ggatcattac gcatatcttc aggcatccct tttaccgatc
     5161 cggtgcgggg tgcatccagg aaatcaagcc ctgtgatcca gtttccgttg accggccctc
     5221 ctatgctctg cacatcattc tgccttccgc tgaagttcca catgaagtac cggaaataca
     5281 tgaatcccag ctgataacgg aacatgaacg agaggttctg accaaaggta ggcacccgca
     5341 gcgttgttgc ctcgccggtc tgtggatcag ttacccgcac cggcttccct ttcgtgccaa
     5401 catacttttc atactccctt acatgatccg gagaatcact ccacatcctc gggaaaacag
     5461 tcataaacct ctcgtcatac accctgttcg tttcacggtt ggtaattata tacctgtcac
     5521 cggaaggatt gtatgtgggt ttgcccttta cataatcggt taccggagca ttgtagtaag
     5581 ccccgtggaa aagcgggcgc cggccatact gctcacggtt aagataataa agcagcgcga
     5641 aggggttcga aggattattc tcatttatcg gcggatttgc ggatgacctg acaacaatga
     5701 cagcgaagct cgagtaccct atcacaatga ccataagaga ggtcaatatt gtgttcagag
     5761 ccactttctg ctttgtggag agaagccaga ggccatagac tatgacagcc aggaaaacga
     5821 tattgagcac cagtgatccg gtaagcagcc acactccaga caggagaaga gttatacctg
     5881 caagtatgaa tatcctcagt ctgtcactgt gtgtgaagct gtaccataca gagagcatca
     5941 gcgcgacaat gaagagcaac aggtggatat acatgccact gttgaacggc agcccgaggt
     6001 tattgacgaa gaatagttcg aatgcggaag tcatcctgac aataccgggg ataattccgt
     6061 acatgataag cacgagcaga agcatcgata cggctattga aaggataaaa cccttccatg
     6121 aaaagctgta tttgcggaaa taccatataa agacaatggc tggtatggca agcagattca
     6181 gcaggtgtac gcctatggaa agacccatca tgaaggcaat cagtatgatc catctgtctg
     6241 catgcggctc gtctgccaca tcctcccatt tcagcattgc ccagaatacc acggcggtga
     6301 agagcgagga ggtggcataa acctctcctt ccactgccga gaaccagaat gtgtcggaga
     6361 aggcataggc cagtgaacct gtcaggccgg caccgaggac tgcaatgatc cgtcctgcgg
     6421 tatagtcaga ttcctgcttc agaaagattc tccgggcaag atgagtgatg ctccagaaga
     6481 gaaacagtat cgtcagcgcg ctggccagcc ctgacatggc gttgatcatc atggctactt
     6541 ttgacacatc gcctccggcg aaattggcaa aaaatcttcc gagtatcatg aaaaaaggag
     6601 ctcccggggg atgccccact tcaagcttga aggccgtagc aatgaattca ctgcaatccc
     6661 agagactcat tgtcggttca atagtcatca ggtaggtaac tgacgccaca acaaaaaccg
     6721 tccagccaag gatattatcc caaagcctgt atttctttat catattcgtt ttatttccgt
     6781 ttctgatgaa taccgcacga aaataataag attttgccga gtacctgtag atgtcggccg
     6841 attttggtat ttttggacaa acagccctgc atgaagagat ttgttattct gctcataatg
     6901 agcctgtttt cactggccag ggccggagcc caatactatg ataccgggca ggatcctgcc
     6961 tctctcaggt ggcttcagat acgcacacct catttcagga tcatctatcc tgacgatttc
     7021 ggcggggagg cattccggta tgcacacctg ctcgaagagt catttgaaaa gctctctctt
     7081 ctttacccgg gggtaaggac aaacatcccg gtaataatac ataaccactc gatgcagtcg
     7141 aacggttatg tgagctgggc gccccggagg atggaactgt accccctgcc cggtcaggat
     7201 aacctcccga tgcaccccgc agcgcagctt gcagtgcatg agacggtaca catgctccag
     7261 ctgggctccc tcaacagccg tggctttggc agggcgctgc ggttcatcct gggggaacat
     7321 gcggtgggcc tcagcgcggt gatgataccc ttatgggcct tcgagggtga cgcggtctac
     7381 gccgagaccg caacaacccc ctcgggcagg gggaggagca acgccttcat ccggggcgcc
     7441 atggctcttt ccctgaaacc cgggggaata tacggctacg acaagatgct ttcaggttca
     7501 tacaggaact tcacaccaaa ccattatgtc ttcggatacc tgatgatgaa ccacctaagg
     7561 acaattgacc ctgacgcctg gaatgaagtt tacaggattg catcgaacgg cttacccggt
     7621 aacccggtaa atgcaggact gagaaaagag acccggctca ctaaaaggag actctatgat
     7681 gccaccttcg ccgcactggg aaaaagctgg agagagagca tgcctgaagg agtgaaggag
     7741 tacaccccgc tcagcttacc cgggaaaagg aattatgtca gccactatac cccccaccgg
     7801 atggatgacg gaaggataat atcactgcgc acatcactct ctgatccgtc acgattcgtt
     7861 attaccggta acagtggtgg gaaagagctg aatattacca ctaccggata tatatatccc
     7921 tgtttttttt cattctcggg caacactata gtatgggctg aacaataccc tgatataagg
     7981 tgggataacc gtgactactc ggtaataaag aggcttgatc ttccggatgg cttaataacc
     8041 gatgtaacca gccgcacacg gtataccgcg ccggacctgt cacctgacgg gcgcaccata
     8101 gttgccgttt caaccactcc tgacatggtc tgttccctgg tcttcctcga ctctcatacc
     8161 ggagaggtgc tgatggacct cgttccgccc gacggtctca tactccagcg accggcatgg
     8221 tcatctgacg gccgccaggt caccatggtc accctgaacc agcagggaga gggaatcaga
     8281 acatacaggc ccaccggcaa gaaatggacc gtgaacctgg aggagagtca tactgatatt
     8341 gtacaggcga agatccataa tgacactctc ttctttcttg cacagggaga cggctctgac
     8401 aatatttacc gcattgcggg ctccgggccg gtggaacgcg ttaccggctc ccggttcgga
     8461 atttccgggt tttcagtcag aggcagtgag ctgcttttct ctgactatac cgcagacgga
     8521 ttcgtcatcg cctccgaaaa gagttcggct accgccggac cggcatttat gaccggccat
     8581 gagattctcc cgccggtggc gccaatgccg ggggaggcgc cggaaaagga gccgccacag
     8641 caggtggcgc ccataccggg aacagaaccg gcatatgagc aattgcccca ggtggcacat
     8701 atgccaggag cagaaccggc aaagaagtct ttaccccagg cggcacccac gccgggagca
     8761 gaaccggaaa aggagccgtt gccggagata acgcccaacc cgggcggggc acccgcagag
     8821 aacattttgc ctgacgtgac atctccaccc gctgatgact ccgtgagtga cgcaaccatg
     8881 ccgttgatcg ctgaacccgg gccataccgg aagattgcaa acctgttcaa tccccacagc
     8941 tggctgccgt tctatgccga tctggatgag atccggaccg atccagccac cattaggccg
     9001 ggtctgacac tgatgtcaca gaaccacctg agcaccctta tctccaccgt gggatatgaa
     9061 tacagtgaag gcaaccacta tctgcactcc ggcataacat ggaaagggtg gtacccggtt
     9121 attgacgcag agataaaatg gggaggagag cagcttatca tcagtgatac cagcgcgacc
     9181 ctgccgccgg agaatccggg aaccgacctg cagcttaacc tgagcatcta tgaccagttg
     9241 tggttcgccc gcgggaaatt cagacagatg gtgatgcctg ccctttacat cagctaccgc
     9301 aacagggata cctggttatc agacgagaac cggtatgaca gggacgtact gactctcacc
     9361 ggcaggttct atttctcaaa catcttccgt acagcttatc gtgacatcaa cccgaaatgg
     9421 ggacaggtgt tcgatctgca gctgaccaca acaccatggg acacaaaact gtacagctcc
     9481 aaaagctacg cccggacaat cctcttcttt cccggcgcac tgccgaacca cagtctctcc
     9541 ctcagggtcg gacgggagaa ccaggccccg gcacggaaac atctttaccg caataaactt
     9601 ccatggcccc gcggttatga tcacaacctg gttgccgaga aacttctctc cttctctgct
     9661 gactacacaa tgccgctctt ctatcctgac ctggctgcag gcagcttcct ctatctcaag
     9721 agaatcaggg gcacactctt ctatgactac tcaaaggggg tggacatacg caattatgca
     9781 gacaggagct ttcacgccgg cccaaagagg ttctgctcag cgggatcgga actgatggct
     9841 gatttctacc tgctgagaat cccctttgag ataagcgcgg ggatacgggc aggctatata
     9901 ccgggtgaaa acagatactt tgtgaacggg gccttttcag tcaacatcta cggcaccgtt
     9961 ctgggacgag agcgctgatg ctgtctccct gatgaggtcc caagcctgcc gtacatgccg
    10021 ttccgttacg ccggtctggg ccgtcaccat cctcagaaca tacctgccat caacctttgt
    10081 atgggtgaga taaatctttc ccgtgtcatt gagggcatgg tttattttct cattcagagt
    10141 gttggcatct tcatcgccca tgccggcagg acagtaccgg aagcagaccg tattcagcgt
    10201 caccggggcc ataagcctga aatccctgtc agcctcaatc agcccggcaa gcatcttcgc
    10261 aagccttaaa tgctcgcgaa tcaatgactg cagcccttca acgccatagg aacggatcac
    10321 tgcccacagt ttcagcgccc tgaagcgtct cccgagagga atgccccagt cgcggtaatc
    10381 attgacctca cccctggtac gggttttaag gtactccgga agtatctcga atgagcggat
    10441 cagcgatgct gcatccctga caaaatagac agaacagtcg aagttggtga acatccactt
    10501 gtggggattg aataccagtg agtcagcata ttcgatcccg tcagccatcc accgcatctc
    10561 gggaagcagg agggctgttc cgccaatggc agcgtcaaca tgcagccaga ttccatgttt
    10621 gcggcatatc tcgcccattg ctctcagcgg gtcaatggca gtgactcccg tggtgcctag
    10681 ggtggcaatg gcacagcagg ggatgtaccc gctggcaata tctcgctcta ttgccctctc
    10741 cagagcctca ggatccattg aaaagtcact cctgacctcc accctcacca ggttgtcgcg
    10801 tccaaatccg gcaatcccgg cccccttctc aaccgatgag tgggtctggc ctgagacata
    10861 tatgcgcata cggcccgtat cagagagtcc ctccctgttg gaacggtaac gtgtgacttt
    10921 ctcacgggcg gtaataagcg ctgccagggt agatgatgag gcgctgtcct gaataacacc
    10981 ctcaaattcc ggcggtattc ccatcattga cctgagccac tccatcatcc tctcctcaag
    11041 ctcagtacct gccggcgagg tctcccagag catgcactgc gccccgaggg cggccgtaag
    11101 catctcggca agcactgaag gaggactgct gttcgcaggg aaatatgcaa agaaattagg
    11161 actctgccag tgggtgatcc ctggcatgat tacttcatca aaatccttca taaaaagatc
    11221 aaaggattcg cttcctgaag gcggaagctc cggcaatgcg ttctttatct ctcccggtct
    11281 gacacctgat tttaccgggt attgctcaac gttctcaaaa tagcgggcta tccactcaac
    11341 gatctcatgc ccgtacctgc gaaattcgtt gctgtccatg cccctctgct actgaaccac
    11401 ggagtcggcc aggagaatta tcctgttcct cttgacctca ataacgccgc cggtaatctc
    11461 aaacctcacc tcggtgccct ggtcatcggc aatgatcacc ttcccggcat caagggttga
    11521 gatgatgggc gcatggtcct tcagtacctg gaatgagccc tttcttcccg gcactctcac
    11581 cgagcgtatc tctcccgaat agatgttttt gtcaggtgta atgatctcaa tcttcacgtc
    11641 cggctttttt acttgttacc tgctctctgc aatcagcttt tcgcccttgg cgatggcatc
    11701 ctctatggtt cctacaagca tgaaggcagc ctcggggtac tgatccacgg cgccgtcaat
    11761 tatcatgttg aaccccctga tggtatcttc tattgacacc agtgtgccgg gaatgtttgt
    11821 gaactgttcg gccacatgga acggctgcga gaggaatctc tgcaccctgc gcgcacggtg
    11881 gacgacaagc ttgtcctcct cggagagctc atccatgccc aggatggcga tgatgtcctg
    11941 caactcatta tatctctgca gtatctcctt taccctctgt gcgcagcggt agtgctcctc
    12001 gccaacaacc tcgggggtaa ggattctgga ggttgagtca agcggatcaa cagcggggta
    12061 tataccaagt tccgagattt tacggctcag caccgtggtt gcatcgaggt gggcaaaggt
    12121 tgttgcaggg gccgggtcag taaggtcatc agcaggcaca taaacggcct gaactgaagt
    12181 aattgatccg tgacgcgtag aggtgatcct ctcctgcatc acacccatct ccgttgcaag
    12241 ggtgggctgg taacccacgg ctgacggcat tcttcccagc agggcagaca cttctgaacc
    12301 tgcctgggtg aaacggaaga cattatccat aaagaagagg atatctcttc ctgttccctc
    12361 accctcgccg tcacggaaat actctgccac ggagaggccc gagagagcca cgcgtgcgcg
    12421 tgcgccggga ggctcgttca tctgaccgaa aacaagagcc agctttgact ccttcagctc
    12481 ttcgcggtca acgaggtcaa tgttccagcc gcccttcgcc atatcctcaa gaaacgcctt
    12541 gccgtatgat ataacgttgg cctcaagcat ctcacggagg aggtcattgc cctccctggt
    12601 acgctctccc acaccggcaa agactgaaag accggaatag gcctttgcaa tattgttgat
    12661 aagctcaagg atgacaacgg tctttcccac gcctgctcct ccgaagagac cgattttccc
    12721 tcccttggag tagggctcaa tcaggtctat aacctttata ccggtgtaga gcacctcctt
    12781 ctcagtcgac aggtcctcat atttcggagg cttgcggtgg atgggataac cacccttctt
    12841 gtcgaggggc ccgatgccat ctataggcat acctgttaca ttcatcagtc gtccccttat
    12901 ctggtcaccc accggcataa ttatcggctg tccggtagct acaacctcca tcccgcgctg
    12961 cagcccgtcg gttgaatcca tggctatggc cctgacggtc ttctctccta tgtgctgctg
    13021 acactccaca accagtactg atccgtcatg acgtttgatt tcaagcgcat caaggatgtt
    13081 tggcatatcg gttccggccg tatcgaaggt aacgtcaacc acaggcccga tgacctgact
    13141 tatcactcct gttttctttg ccatctctct gacttaaatc tgtttctatg taatctgata
    13201 atatactctc cttattcttt gcccctgacg gggcgaaatt cattttaccc ttccgatgag
    13261 attcaccgaa agctgtttca gttcgctctt tctctctcca tttgccgaca cctgatcaag
    13321 ggcctggaat gccctgttga agtagtcata cgcaagattc tcagcctgca gccttatctc
    13381 cagttcatca tagatatccc tcacacccct gactttcgct tccgggtcac tgctgccggg
    13441 cccgagaagc tccctgaggc gggtaagcat gctgccgccg gccatctcaa aggcctttac
    13501 caggaggtag gtcttcttgt ttgccactat gtcactgccg gtcttcttgc cgaacagctt
    13561 tgtatcgcca taggtgtcaa gaacgtcatc ctgtatctgg aaggcgatgc cgaggcagcg
    13621 cccgaattca taaagcaggt cctgatcctt ttcggaggca tctcctatga ttgctcctgt
    13681 cttgagggaa gcagcaatca gcactgcggt cttcagctct atcatgcgaa ggtattcggc
    13741 ttccgacaca actcccttct tctcaaagtc aaggtcgagc tgctgaccgg tgcatacctc
    13801 aatggcggta cggttgaaga gcttcagcac cttcggcagg gccctttcag gagcctgacc
    13861 tatacactcc attgcaatga atgccattac atcacctgac agcacggcct gatccgggcc
    13921 ccacttcgcg tgcacagtcg gggacccgcg ccttacctca tccttgtcaa tgatatcatc
    13981 atggatcagg gtgaagttat gaaacacctc tatgcccacc gccggcatca ctgcctcgtc
    14041 aatcttgtca ctgaagatgt tgcatgacaa aaggcacata accggtctga gtctcttccc
    14101 cccgatagac aggatgtatc ttacgggctc aaagagcctc tctgcctccg gtgtgccgcc
    14161 gagactggct attgccttgt ttgtcagttc tctcagatat tcgctggtga acatgtctct
    14221 ctctttccgt tatccgttca gcgcttcagc gccgctgacc acctcaagca gctggttggt
    14281 gatggaggcc tggcgggcct tgttgtactg cagggtaaga tccctgataa gttccgtggc
    14341 attgtctgta gcctgatgca tggcagtcat acgggcaccg tgctcagccg cgaacgaatc
    14401 aagcagtgag cggtacaatt tgatgcgcaa tgtcttcgga atgagctccg agatgattct
    14461 gacctggtca ggctcgtaga tatagtctgc aggcgcggag tcctgacctg acatggttcc
    14521 catcgtcacc ggaaggaaca cctcgttggt gagcttctgt acagctgcat tcttgaactg
    14581 gttgtaaact atttcgatgg tatcatactc cttcgacacg tagagcctca tcagtttatc
    14641 agccagcgca gaaattctgt caaatgtcag ctccgtgtaa aggtcactct gatcctctat
    14701 gatgttgcac ttaagtttcc tgagtgcgtc attcgccttc ttgcccactg tcatcacccc
    14761 gagatcgccc ctgctgttct cctggctgta tctttcgtcc atgatacgcc tggtctccct
    14821 gagcacgttg gcattgaatg ccccgcaaag ccccttgttg gatgtgacca ccacaatcag
    14881 aacctttccg ttgccctttt ttctttgagc atatatgttt tcggtctcgg tatcgcgtaa
    14941 tgcagcgcag acacttgcct gtatctcatt gagcttatcg gcataaggcc tgagctgtac
    15001 tatcgcgtcc tgtgccctgc gcagtttggc ggcagccacc atcttcatcg cagaggtgat
    15061 ctgcctggtt gaccttaccg agctgagcct gatccttatc gctttcagat tagccatcct
    15121 gtctctccat ccttttgact agcgtgactt aaactgtgat gctacctcct ccacagcctt
    15181 gtcaagcaca ccggtcactt catcattgat gattcccttt ttgattgttt caagcacatc
    15241 actgtgattg ttacgcatgt acgccaggaa ttcgttttca aactcgctta ccctatgcac
    15301 cggcacatcc ctgagcaacc cctgcgtacc gcagtaaatg atggcaacct gctcctctac
    15361 cttcacaggc gagtactggt tctgcttcag tatctcgacg ttcttggcgc ccttgtcaag
    15421 cactgcaagg gtagcagcat cgaggtcaga gccgaatttc gagaaagctt ccagttcacg
    15481 gtactgtgcc tggtcaacct taagtgttcc ggccactttc ttcattgatc tgatctgggc
    15541 gttaccacct accctggaaa ccgatatgcc cacgttgatt gcaggcctga tgcctgcgtt
    15601 aaagaggctt gactccagga agatctggcc gtcagtgatc gatatcacgt tggtggggat
    15661 atatgccgcg acgtcaccag cctgggtctc gatgatgggg agggctgtga gtgacccgcc
    15721 acccttcacc atatggcgta ttgattcagg caggtcattc atccttgctg ccacctcatc
    15781 tgattcaatg atcttcgctg ccctctcgag aaggcgtgag tgaagataaa agacgtcgcc
    15841 cgggtaagct tcgcggccgg gcgggcggcg cagcagtagt gaaacctcac ggtatgaaac
    15901 tgcctgcttg gagaggtcgt cataaataat cagggcatca cggccggtgt cacggaaaaa
    15961 ctcgccgatt gcggctcctg caaagggagc atagaactgt gctgccgctg aatcagcagc
    16021 agtggcgctg acaatgacgg tatagtccat ggcaccatac tgctcgaggg tggtggctat
    16081 gtttgccacg gttgatccct tctgccctat tgccacatag atgcaatata acggtttgcc
    16141 cttctcatac tcgcttctct ggttgatgat ggtgtcaatg gcgatagccg tcttgcctgt
    16201 ctgccggtca ccgatgatga gctcacgctg accacggcca atgggtatca tggcatcaat
    16261 ggccttgagg cctgtctgca ccggttgctt caccggctgg cggaagatga cacccggcgc
    16321 ctttctctca aagggcatct cgtaaagctt ccctgtaatg ggaccacggc cgtcaatcgg
    16381 atcaccggtg gtggtgatta ccctgccaag cagaccctct cccaccataa tggatgagat
    16441 tctgccggta cggcgggcaa tgtctccctc cttgatcttc tccgaggggc cgagaataac
    16501 agctccgaca ttgtcctcct caaggttgag aacaataccc ttgatgtttg actcaacaaa
    16561 ctcgatcagc tcgtttgatc ctacattctt caggccgtag atgcgtgcaa tgccgtcacc
    16621 gacctgcagc accgtcccca cttcctcaac atcaatggcc gtctctactc cttcaagctg
    16681 ctgccttaaa atctgtgata cctctgaagg tttaatatta gtcatatctc tgaatcttat
    16741 aaactactat tcggtaaatc ctgccagtga aaactccttt ctgaatctgt tcagcctgtt
    16801 ccttacgctg gcatcaacgt atgagtcatc aaccctgaga ataaatccgc cgataattga
    16861 ctcatctatc tgctcattga actcaattgt gcctcccttt tctcctgtga taaggtcagc
    16921 cagctccctt tttgtggtac catctacggg aacggctgtg gtgacggtca cctggcgtat
    16981 gcccttgtga cggcgggtga ggtcaatgaa gttccgtgct gctgcagaga ggtaatcgcc
    17041 ccggccgttg ctgaacatca gattgatgaa atcaatcgtc tgcttttcag catcatcacc
    17101 ggcaagggca atgattgcat cctttctctt ctgctgcggg agaacaggat ttctgatcac
    17161 atttttcacc tcttccatgg cacaaaggcg gctcaatacc ttcatgtcac ggtaaacgcc
    17221 gtcaacagtg ccgctttcaa gggcaaggtc aaagagagcc ttcgcatatc ttaccgctat
    17281 cctggctgaa ttcattggat cagttcctgt ttgcttctat ttcgttaata tatctttcta
    17341 tcagcttctg ctgctccgca tcgcttttaa gggtctcacc aaccactttc gaggctacct
    17401 cgatggccag cctggccacc tccttcttca cctcggcaat ggcagtcacc ttctcacgct
    17461 cgatgagctc cctggcacgg cgcaccagcg cgtctgactc tgactgcccc ttctccctgg
    17521 cttcggccat catacggtca taggccacgc gggcatcacg aaggatcttg tcacgctcct
    17581 cgcgcgcctt ttgcaggatc atctcattgt cagcctgcag taccttcatc tcctcacggg
    17641 ctttctcggc tgactcaagc gaatttttga tcatgtcttc acgcgtcttg acggctttca
    17701 tgataggccc ccatgcaaac ttccaaagca ggacaagcag gaggaggaag ataagcgttg
    17761 tccagaatat tgtcccaatg gcaggactgg caagactgct ttgtagtaac attttatgct
    17821 gtttttaaag atttacctgt aaagcctgca accaaccgtt gcaggctttt tttgatttta
    17881 gcccttaatc attgcgacaa cgatagcgaa gagggctgcc ccttcgataa gcgctgctgc
    17941 aatgatcatt gcggcctgaa tcttcgagta agcttcaggc tgacgtgcga tggcgtccat
    18001 ggccgagccg ccaatacggc cgatgccaag acctgcaccg ataactgcaa gccctgcgcc
    18061 tactcctgct aatgacatta ctgcttgttc cataaatcaa atgttaaaat aaatagttga
    18121 tatattgata attaatgatg ttcgggttcc gctgtggcca atccgatgaa gagagccgac
    18181 agaagggtga atacgtatgc ctgcaggaaa gccaccagca actcaatgca atccatgaag
    18241 agcacgaaga tgacggaaaa aggagctaca gccaccgttt tgaaaatgaa tatcagcgtc
    18301 acaagactca gcacaattat atggcctgca gtgatattcg caaagagacg gaccataagt
    18361 gcaaaaggtt ttgaaaagag ccctatgatc tctatgggta ccatgacggg cagcagccag
    18421 aagggcacac cgggaggagc aaagacatgc ctccagtgtg acttgttggc actgaactgt
    18481 gttatcagaa atgtgaatag cgccagaaca aaggtcacat tgatattgcc ggtgacattg
    18541 gcgccaaacg gaaagaccgg aatcagcccg agaaggttgt ttataagtat gaaaaagaaa
    18601 acagtaagca ggtagggcat gaacttcgca tgctttttat cgccgatgtt cggaatggca
    18661 atctcatccc tgataaagag aatcaccggc tcaaccaccc cctgaaaccc tttcggcgcg
    18721 gcagtgcctg ctcttttgta gctccgtgcc agtgaaagca tcagccataa aagaacaatg
    18781 gcggccacaa tcatcccgaa gatggccttg gtgatcgaaa agtcaagggg aggtttggaa
    18841 ccgtcatgct gaccctcctg gtcaaccctg atgatcttgc ccgcatattt gccctcatgg
    18901 gctatggcaa aacccttgta gctttgacca tgagctattc tccgcgatga gaagagatga
    18961 aggcctgact ccctgtcata aattattacc ggcaggtaaa cggctacact tgttccatcc
    19021 ttcttcgtca ggatatgcca ctcgtgagag tcggcaacgt ggtccatgat gaagggagca
    19081 gcatcaaatt cctcttcgcc gccgtgcgct ttgccttctt tatccatgat gcccgatgag
    19141 aacgccggaa cagtacctgc cagcaaacca agcaccagaa gcagaagatg taaatacctg
    19201 tttttcaagc tgaatttctt aaatctgact tctttttcag aacacccgta aatgtaagaa
    19261 ctacatatac tgtgattgcc aaatatagaa taaaaaacaa tactactgaa cctgttgatt
    19321 tgtttttaaa gacaagaaag aaaagcagcg caagcacaaa acaaagcacc atcttcaatc
    19381 caagtgcaat tagtgtcata aaaacacttc tttcccggtc agagtggtat ccgttaaaaa
    19441 aaattaaaag tgcaatgaag gctgtcaggg tgaagcctgc agtgagaaaa gcggctgcgg
    19501 aaatgttgaa gagagagggg aaaagccttc caactaccat ttccatgatg atgaaaaaga
    19561 tggcgatggc gccaaggccc aggtagaatc tgactgccag gggtttaaac atctccctta
    19621 tttcttaaga cctccttgat tacgagccac atggcagacg caacgcccag cagtgacagc
    19681 acaaccgtaa ggattggttc cgtgccggcg agtttatcaa gtttgactcc gccgagtgat
    19741 gtgataacga taatgatcac catctggatg gcgatggtgc tgtatctgga gtaggcgtca
    19801 aggccctgct tgcctctatt taccttgttc tttgggtttg gctgctgctg ttccaccgtt
    19861 ttcaccctcc ttcatgacgc agttgccggt aaatacagct cccggttcta ttgacagctt
    19921 gttggtaatg atggtgccgt ttatttttga tgaggctttc aggttaagga gctgtgacac
    19981 ggttatcttt ccttcaatca ggcctgaaac ttcagagttt ttgcattcca cttccccctt
    20041 aacctttccg gtggggccga tgaccacctt tcccctggtg ctcatgttgc cgttaaggat
    20101 tccgtctatg cggatatcgc cggttgattt tatatcaccc gtgatctctg ttccctggct
    20161 gatgaggttg actgcattgc tgttgtctgt ctcaattatt tttgccatag taatctgttt
    20221 attcctgatt ccccttaaaa ttatttaaca cgtaaatata aaaacttatt gtgccggatg
    20281 agtaaaaaag gggagtaaaa agccagggtg caaaaatctc tttcatcagg ccgggtcata
    20341 ggcccacctg agccatatgc tgccccatgt gaatcctcct ccgaaagctg ccaggatgag
    20401 gttgtcgccc ttcccgagtt tcttctcgaa ctcccagatt accaatggta tggtggcggc
    20461 ggtggtgtta ccgtactttt ggatgttgat catcaccttt tcagggggca gtcccattct
    20521 ccgcccggtg gcgtctatta tcctgaggtt tgcctggtga ggtacgagcc agttaatgtc
    20581 atcggggccg aggttattct tttccatgat ttcgaccgcc acatcggcca tattggatac
    20641 ggcgaacctg aagacagcct gtccttcctg gtatatataa tgttcccgtc ctcccacggt
    20701 atcatgggat gcgggcctca cagagcctcc tgccttcatg tgaaggtatt ttcttcctga
    20761 cccgtcggtt ctgaaaatat aatccatcac acccagctct tcgtatgtgg gttccaggag
    20821 tactgctccc gcggcatcac cgaagagggt gcatgtggtc cggtcagtat agtcagtaat
    20881 agacgacatc ttgtcagagc cgaccactat cacctttttg tacctccctg tctcgacgta
    20941 tgcggctgct gtctggagtg cgaaaaggaa cccggagcag gcagcgttta tgtcgaagct
    21001 gaatgcgttt ttaatcccga ccttgtcgga aatgatgttg gccgttgccg ggaatgccat
    21061 gtcaggggtg acagttgcac agatgaggag atcgatctcc tccgggcgtg tgcctgtctt
    21121 ttcaagcaag cctctgacgg caggctcacc caggtcagat gtgcccttcc cttccccctt
    21181 caggattctt cgctccttta tgccgacgcg ttgcatgatc cactcatcgg tggtttcaac
    21241 catcccggac aattcctggt tggtgagccg gtattccggt gtccaggcat tgatgcctgt
    21301 tatagctgcc cttattttag tcatgctccg aatgcttccc tgatctttcc tgcaagatct
    21361 gcctgcacca catcacgggt ccggagcagc atattcataa tggccatacg gtttgatatt
    21421 ccgtgtccga ccactacgtt ttcatttatg cccagaaccg gtgtccctcc caccacctcg
    21481 aaattgagtc tgtcaaaaaa gctgtctttg atgccttttg accttgctac aacatatagt
    21541 ttttcagcca ttttaagaac cacatttccc acgaatccgt cgcacactat tacgtcggcc
    21601 atctcatcgg tgaagaggtt gtggccttcg acattgcccc tgaaggtgac atcactgctt
    21661 tcactcagga gttcatatgc cgctttcact gctgcagacc ccttggcttt ctcctttcct
    21721 atgttcagga ggcctaccgt tggttctttt acgccaagca catgttttgc atatacggag
    21781 ccaagtattc cgtactggag caaaacgtca ggtttgctgt caggattgat tccgacatca
    21841 agtatgattg aagatttccc gttaatattt ggtatcagtg ctgcgagtgc cggtctgaaa
    21901 acgcccggaa tgacattcac ggtataactt gctcccacca tcattgctcc ggtattcccg
    21961 gcgctggcaa atccgttgat gaggcccttc ttgagcatgc catacccgac cgcgatagac
    22021 gaatcacgct tctgcgaaaa cgccttggcc ggatggtctg acatctcaat tacctgtgat
    22081 gtaggcacaa tatcaaaaag agaggcgtct gcatcaagcg atgcaagctt ctcccttatc
    22141 cggccgttgt caccgataag cacaatacgg tcacctgacg acatctgtcc gagtgcgtca
    22201 aatgctccct caataacagc attgggagcg taatcacctc ccattgcatc aagacctatc
    22261 ttcatcgttc agattttttg aacggaaaga gcgtcaggct gtcgtcttct tctcaacggc
    22321 aagctttccc ctgtagtatc cgcactcagg gcagaccctg tggtagagat gggctgaccc
    22381 gcagtttgtg catgcagctg ttgtgggagc cactgccttg tagtgtgtcc tcctcttgtc
    22441 tctcctggta gcggagtgtc tgtgttttgg atttggcatc gcagtaatga attgagttaa
    22501 ttataccctg tcagtttttt cagatcatcc cagcgcggat cggtttgtac ctcaccgtga
    22561 acaaggagat cattcagtcg cctgatcatg tccggattgc aggtgctgtt gccatcggca
    22621 tcatcaggat gaatgcgctt aatgggtaac gccaggtgga tgtaatcata gaaaaactga
    22681 ctcagatcta tttcattttc atccatcggc atagtaatca tgtccggatc agattcctcc
    22741 cactcactcc cctgccttac aaaaagcctt ttggcagttg agactggcat gtaaaagggt
    22801 tcaaggcatc tgtcacagat cacctcggcc ttcccgttga tgactatgtc aaactcaata
    22861 tgcgtcgaac atttctgaag caccaccacc gcacgaaact ctcccttctt tatctcagac
    22921 tcctcatata acccgaagag atggttccct atctgaaaat cataggtata tctggcctcc
    22981 tttagtcccg cgagcggtat ggaatacaat ccgggcatct tctttttttt cggtctgcaa
    23041 aattataaat cctcttccaa ataaaaaaat tatgaacaaa aaagtaacac cgcctgtttt
    23101 cttcgctgtt caccggtgtt tatccgctcc ctgccggaaa tgagtggcac ggattgaacc
    23161 agcattaata tttttgaggc cagaatcaaa tcagtcacca tgaaagagaa acacggtaat
    23221 ccgttcacag gcatagcaaa gttcctgata tcactgggaa ttgttatgtt cgtcgcaaag
    23281 cttgaccttc tcgggctggg agatattcct gaatatttca gctggcagat gctgctcatt
    23341 tttttcggag tgtggttgct cctcagcttt aaacttgtgt cagcagttat cttttttgca
    23401 ataggattct ggtttctcat gccggatatg gccatacaac tcaccgagtc ctataaaaag
    23461 atctactggc cggtggtgct gatcctctgc ggagtggcat tcatgctgaa accacttgca
    23521 aaaagttgca gataacagta acatttaatg attaacaggc ccgtaaatat ttgttgacag
    23581 acatatatac aattaattgg aatgtatatt tcacaaaaga taatcaatcc tgtcaatcag
    23641 gcagttataa gcaatcattt acaaacatac caaaccattg ataatcttaa actatggaaa
    23701 atgaattgaa aaaaccggca agaaacccgg aaacctcgaa caggttcatc ataggagtgc
    23761 ttctgatcat cgcaggactg atccttatcg tcaagaaatc gactgtgttg ccggaacccc
    23821 ttgatgactt cattgataac atcatcttca gctggcagat gctgctgata gtgataggca
    23881 tcatcgtcct caacggatca gacaacaaga ctcccgggat tgtaatgatc tccgtgggag
    23941 gattcttcct gatcccggaa ctattcaccg acttcttcag gtcattcaac ttcttctggc
    24001 ctgcactctt catagtactt ggtattgtgc tcctggtgaa ctcaaaacgg ctggtgaaga
    24061 agctcgactt ttcaacggtc aacaaggcag attatattga ttacgtgaat gtcttcagcg
    24121 gagccgagcg gcaactcatc accgataact tccggggagg caagatcact tctatcttcg
    24181 gaggtggtga ggttgacctg acacactcat cactctctcc gggtgacaat gtaattgaga
    24241 ttacctgtat ctttggggga acaaccatca ttgttcctga aagctggaac gtggtgattg
    24301 aaatcacacc cattctcggg ggattttctg acagcaggaa gatgaggggt gatgtgatca
    24361 gggataatac gaggtcactc accataaagg gtaccgtgat gttcggaggt ggagagataa
    24421 agagttacaa ataatttatg aagcatccgt tactggaaaa cagaacgagg ttctttgtgt
    24481 ggtggctggc ctggcttatt cttgcagcag gccagtcact ggttattcat tttggctatg
    24541 gaatccctgc cgatacggca tttgccgacg gagtgatttc aatgattttt ttcggcatcc
    24601 tgggactggc tgtatggttt cctttaaggt tcctcctgaa ggaggataac caggtctata
    24661 caacaattgt aaacgtcctc ctgaccggtt cactgacgat tcttgcatgg ctcttcgggt
    24721 cgaagatcat cgtaaggatg cttgttgccg aaaaggtgga ttatgacatc ttctggcatt
    24781 ctgtcctgat tttcagggct accgccggtg tgctggtctt cttcgtcatg atacttgtct
    24841 actacctgtt cctgagctcc tcgaggctgg ctgaaaaggt ggcacggcag gcccagcttg
    24901 aagcacaggt acgcgaaggc gagctgaaga tgctccggtc acagataaat cctcacttcc
    24961 tgttcaacag ccttaactca atcagctcac tcaccgtgac agatccgctg aaggccaggg
    25021 atatgattgt aaagctgtct gaattcatgc gctattcgct ctcatcaagg agcgaacagc
    25081 cggtgaccct cgggaacgag atggagagcc tcaggcttta cctgcagatt gaaaaggtga
    25141 gattcggtga aaggctaagg attgaagagg atatcaaccc ggaatgcctt ccggcgctta
    25201 tgccgggaat gctgctccag ccactctatg aaaacgccgt caaacacggg gtttatgagt
    25261 cgaccggaga agttataatc agaacaacca tcaggaagca gaatgaaatg gttataatca
    25321 ccattttaaa caatgttgac actgactccg ttgtgaccag gaaaggggca ggcataggcc
    25381 ttaagaatgt cagcagcagg cttgagctgt tctttggcga caaggctgac ctcacggtga
    25441 atcacggaga tgacagcttc acggtgattg taagatttcc attccgaaaa tcatgattaa
    25501 cagcataatt aaaccatgaa aacaatcaga gcactgatca ttgaagacga agctccggca
    25561 agggaactga tcaggtttat gctcgccgat catcctgagg tggagattgt tggtgaatgt
    25621 gtagacggat tctgcggggc cagggagata aaggaaaaga gacccgatct tatcttcctc
    25681 gatatacaga tgcccaggct gaccggtttc gagctgcttg aggtgataga tgaaaagccg
    25741 gaggtgatat tcaccaccgc ctttgaccag tatgccatca gggctttcga actgaacgcc
    25801 gtggactatc tcctcaaacc tttttcaaag gagaggtttg atgatgctct tgccagggca
    25861 ctgaaccggc tggagacaaa ggcgcctgag catgacaaaa ttgaaaaact ggtggctgca
    25921 gcctccgaag cggaagaatt tctcaccagg atagtcttca gaaagggggc gggaatcaag
    25981 atcattcctc tgaacaatgt ctattacctg gcggccgagg atgattatgt gatgatctat
    26041 tacagtgagg ggaaagcgct caagcccaag accatgaagt attatgagga gcatctccct
    26101 cccgacctgt tcataagagt tcacaggtca tttatcgtga acgttgaaca gatcacccgc
    26161 cttgagcctt acagcaagga taactatgtt gccgtgctca ggtcaggtga aaagataccg
    26221 gttagccggg ccggatacaa gatgctcagg gagaaactct ccttctagtc atggtccttc
    26281 agcctgttac acctgggagc cctgcaaagg agtgacctgc atcccctgta ccgcctgaag
    26341 atgaacatgg gaaccggatc atagccgtgt gtgcctcccg gcctgcagcg aagtatcctg
    26401 tcagccgaga tcattattcc cctgaacggc ccgtggagcc tgagcgcatc aatggtatac
    26461 cgtgaacagg taggaacatg acggcaggag gcaggagtcc atggcgatat aatccaccgg
    26521 taaagccata caggcagcag gagcaaaaaa gataatatct ttgcggcact cttcagcatc
    26581 gccttcaaag ataagctgat tcggcatgac aaaatgacct cttttcagaa tctctttatg
    26641 atggcaggcg ttttgctgag gaaactggaa aattaaatta ctctcaactg atggaaacaa
    26701 tgtttttgca aaacggtgta tactggataa tattcatcgt attcatgata ctgagctggg
    26761 ttgtcagcgc cagactcaag tcgaaattca gaacctattc gaaggtccct gttgcatcag
    26821 gcatgacagg ccgtgaagtg gcggaaaaaa tgcttcgcga cagcggtgtg acagatgtta
    26881 ccgttcacag tgttgccggt gagctgactg atcattacaa tccgcttaaa aagaccatta
    26941 acctgagccg cgatgtttac tccggaacca gcgtggctgc tgctgccgtg gcagcccatg
    27001 aaacaggcca cgccctgcag catgcacaat catacgcatg gctgggaatg aggtcaaaac
    27061 ttgccccggt ggtgagcttc gcctccaact ggatacagtg gatactcctt gccggacttg
    27121 ttctgctaaa ctccagaccc gaaatcctgc tgataggaat agtacttttt gccttgacca
    27181 cactcttcag cttcatcacc ctcccggttg agatcaacgc atcaaagagg gcgctggcat
    27241 ggctttcaga tgccggcatt acctccccgg gaacggatga caaggcccgg gatgctctta
    27301 aatgggctgc ctatacgtat gtgatagctg ctctcggctc actcgccacc ctgatctatt
    27361 acatcatgct tttcctggga gcaagggaca ggtagaagcc aggtgtcatg caattccgcg
    27421 gggttacctg cctcccacgt acagaaattt cgtcctcttt ataatgtacc tgattacagc
    27481 ccagaagata agggctgcaa ccagcagggt gacatacatc ggctccaggt aaatcctgcc
    27541 tgtgaccgca atgttccggc agagatcaag gaaggcaaaa acaagaaaaa ggtttgcaat
    27601 acttttgtac tcacgtctga tcacattcct gaatgagaac ggcaggtcag gctttacata
    27661 atgcctgaac gaagggatga cagccttcac ccggaaagcc catgtgtcat atgcttcccc
    27721 gaacttgcgg cgcaggtatt gctccccggc aaagatgata cgctcataat aaagccagga
    27781 ggccagcacg aaaacaatca cccaccatac tgatctcagg aaaagcaccg ggcctagact
    27841 catcaggata ttgccgacat acagcggatg gcggattacc gagtagatac cctttgtgtt
    27901 gagttcgtca gccatctggt caatcagatt ccgctccgag gcgctgcggg gaagatatcc
    27961 gattgtgtag gagcgcaaca gtaagcctgt aaccgagaca aggatacaga ttatctccca
    28021 ggtatagttg aaataaatta cgctgcggtt tgctatgaaa agataaaaag cagccggaac
    28081 aagcattgtt gccggcagcc agctccttct cctgaataac cagttaccgg atttctcaag
    28141 ttcatggaca agcgacattc ttacggtatt tagctgtaaa tgtatgaaaa tttcagaaac
    28201 tataccttat ccgtatgaat ccgaggttta tccgggtttt ctcttcgccg acttccatcc
    28261 cccggcgccg gacacacaaa aaaaatccgg gagagacaac ccctcccgga taagcttatt
    28321 acagacgcgt catatcacca catattcatt ttgctctatt gcaaggaaaa tttgatggga
    28381 atggcgtagt aaacgttaac cgggttgcct ttgatttttc ccggcttcca ttgcggacac
    28441 tggctcatta ctcttacagc ttcgtcatca agcaagggat ccacgcccct gacaacgctg
    28501 acatcttcca catacccgtc tgatgtgact acgaacctga gtgttactct tccctggatg
    28561 ccttttccct ttgcctgatc cggatacttc aacgtgccat aaagccattt gaaaagctca
    28621 gtatcaccac ccggaaactg aggcatctcg tccgccatga tataaacgat gccgttgctc
    28681 accctgtcag gcaattgaaa acggatcact atctcggatg tgaagggttc atctgcctcc
    28741 ctgtttgcag ggcttttcca gcggggtgaa gattccatca ccctggtcag ctcgccgacc
    28801 agtgcaggat cagagtctga aatggctttt atcccggaaa ctgatccgtc tgtgtcaacg
    28861 gtataagcca tccttaccca tccgctgacg cttctggaag cagcatcagc cggatatttg
    28921 gcccgttcag caacccagtc ggcaaaagag aggaaactct cgcccctgaa ggtggggaaa
    28981 tctgcttcat cctccctggg atagggcagg ataatgcccg attcttttgc tttgcttctg
    29041 gaagtaactt caatagctcc cttttccccc ttatctccgt atttggtttt cgctgcagcc
    29101 ggatcaagat aaactgactt accaaaatcc tttcccagtt tttttgtggc ctcctcaagt
    29161 gaaagatctg aagcctgacc gtcaaccatg accagtagtc cctgtgaaac tgaacccatg
    29221 tttgcataac gctggttggc cttcattgtg attgtcgctc cgcggactga cccggcggta
    29281 agggcaaccg gttcgtagcc ggcggcagta aacatgatgc caacatatgc gggcaggtca
    29341 ctgaagtaaa atctgcccgt ctcatctgtg gtaacgctca gggaggtccc cgaaacattg
    29401 acggttactc cagctaacgg agtaccatcc tctttgacca cggtaccctg aatatccttt
    29461 atcaggcttc ccggagcact gtcactatag gtatagaccg gatcagcgaa tgcgtacagc
    29521 attacagcta caagtggcag tacaagaagc aatttgagct tcctgtatgg agaatagttt
    29581 ttctttttca tcatttcaaa cctgtttgtg ttaaacgaaa aattgaacga attggacagg
    29641 cttatcagcc ttgcattgaa gacctggttg agaagtgctg ccctgtaggc tgccgggtcg
    29701 gatgtgcgct gcagcgcctc ctcgtcggcc agatgttcat gattctgcct gaccagggcc
    29761 gaacatatcc atgcaaatgg attcatccac tgcactatgc tcaccagttc aacaagcagg
    29821 agatcaaacc agtgtttctg actgaggtgc accatctcat gattgatgat ctcccggacc
    29881 tcacgcccgc ttagtgaagg attgataaaa acataattga aaaaagagaa ggcgaaggtg
    29941 tttttttctg atctgacgag caggacgccc ccttccctgt ctacctgccc gcttctgacg
    30001 gtgcggcaga ccgacaggag gtacttcagc gtccggcccg acattaaaag tatgcctgct
    30061 atatagaata tcatgaacca tttcagtatg tctgcatgtt ctgtaatctg tgccgcgggt
    30121 gcaggagaag cagatgtcat cccctgccca tcaggtatca gcggagcagg aagctcaatt
    30181 ttgtaatgca gcgtcaccag cggaaggagc agtgagagaa ccactcctgc cagaaggtag
    30241 atccgcttca gcatgaaaaa tctctccttc tgcaaaaaga ggagataaac cagcgtgaaa
    30301 gccgtcagcc atgcggccga tttcagcagg tatagtgcaa cctgttccat gttctattcc
    30361 tgttggtcat catcaagctc ttcccgggcc tcctccagaa tcctgtcaag gtccttcacc
    30421 gtaaggttct tctccctggc aaagaacgat gtcattgccg ggaatgaatt gttgaagtac
    30481 ccctccagca gtccgaaaag ctggtgtttt gagtactccc gtttcgagac aagaggataa
    30541 taaaggaata cattcgcata ggcccggtga tcaacatatc ccttcctttc aaggaccttc
    30601 agcactgtgg atatggtgtt gcgggcaggc ttcggatcgt caaaccgctc cctgatatcc
    30661 ttcaccactc cctcttccag ctcccataaa atctgcataa tctgctcttc agccttagta
    30721 agttgcataa tattatcgtt ttttgatcaa atcttattta tgaatcaatg tcagcaatcg
    30781 ctcatacact ttatcaggac gctcgcaatt ttctgaaatt atcaatccat cggcatcaat
    30841 aattgcatag tgaggtattc ctcttacggc atatttttcc tgaaagtcag cagcccagtt
    30901 cccggtacag ataaggtttg tccctctgag tttttcattt tcaatgatac ttttccactt
    30961 ttcgtattcg tcatcaagac agatatttat aatctcaaca ggcttgccgg aaaggttttc
    31021 tgccagtgtg ttcaataagg gtatctcctc aaggcatggc ccgcaccatg tagcccagaa
    31081 atgcaggtaa atcaatttgc cacggtaatc tgacaatgta tgaaaaaccc ctgcggtatc
    31141 tttaagataa aaataggggg cttcagaacc tggtttcagc ctgttttcat cttctagttt
    31201 cccgagcttt ttcacactct ctgatcttct ttcatcgatg tagctttcct gcaggggtgt
    31261 caacctgaaa cctgacgcaa ctctaaatga atcagccagt tgaaattccc ttctcgtgtc
    31321 aaaacgataa tacagatcat ataaaagata tacattgaaa tattcaagta cctttccttt
    31381 caaaagcgaa ttgatattat ggctcgcctt gctgtaataa gccatcagcc ggggcgaacc
    31441 aatcaaacgg tcatcaaccg tatcagatag taacagatag tcggagataa accgccagta
    31501 aattggagat gagacagctt cagggttata aatctttaca tcgcacggca taggaatacc
    31561 tttcaggttc cggtctttca gatagtaata ttgaaagaac ttcatgtgtg ctgaaaaata
    31621 ggagatttca ttttcataaa aatcacaaaa ccatcccgga aggttattac agtttttctg
    31681 caggaactcc agcctctcct tccctgcctg gtctatcctt tttacagcat ccttaaactg
    31741 tctctctgtt tccggttgag tattgaaagc cgtgatagca atggggctct gggtataaaa
    31801 acagccaaac tcacgtcttt cattttgcag gaaagcaaat attggatcat caatatgaaa
    31861 agagatattg ctgcttccat cacctgcctt gtcaagacca acccgggcaa ccagcgtgtc
    31921 gccaggtatc atgtaagagg tataaaccgt atccctgaac tgaaaccaga cctgttcagg
    31981 ccttgttatt ctgtaccttg cttcgcagac ccttttatga accagggtaa atctgtgaat
    32041 caggcacgac tgtgcaggat aataactgga atgattgata tccatcctga cggaatcaag
    32101 gtcatcctga actataactt tgatataagc cacccctcca aaatcaatct caggagagta
    32161 ttcctttccg ccattacagc cggcgagcag gacaagtgct gaaataaata ctacaagttc
    32221 tctcattccg gaaccgattg taataattca tgcaaaagca actctgtgcc cgtttccgga
    32281 cacccatttt caatcaatcc ttcgccacaa atatatgact atatttatag acgtgcaagt
    32341 ttttttattt ttttcgtcta tttttatagt cgcgccgtga aaaggaacga tccaataaaa
    32401 gtagatatca gatttcaacc ggccttttaa tacaaaaatc cgaagagcca gagcggaatt
    32461 atattacctg acccatattc aatattatct tttacaacaa aagcatttgc agcttttgcg
    32521 atttgtttgc cggatttctt ctttcccccc catttcatat tgtgtacttt ccgtctacaa
    32581 caaagtcccc ttcgtccggg taattaacta tgtgccgggc cgagacctgg tttagaaaaa
    32641 acgttagagt gtcatttatt tatttcaaca ctgtaatgtt tcattgatac ctgttattct
    32701 gatttactgc ctggctgctg tcagaatccc tcaactatct gcaaaagctt ctcccgctct
    32761 gtactgccgg gggctgactg cagaaggtca ttgagccagt tgccgtacat ggggttggga
    32821 agcacgatat atcttgtgcc gaagagcctg cggtctgcat caacagcccc gaagccgtgg
    32881 tcaggccccc ttttctcata aatgccgctg aaatcagcca ggttgtcacc caccagaagt
    32941 ataatctcat aatccttttc aataaacgcc cgtctctcca cctttgagga gacgctctcc
    33001 ttcagcagca tgtgagtgct gtcagcattt gcaaaacccc acgccgcaat atttgcaata
    33061 gtcggcccca tctcctccac ggttctgttt gacacgtaga aaacctcaac gcccagggaa
    33121 tcggcatacc tggtgaactc caccgagccc ggcagcggtc tggcgcttgc aagcgccacc
    33181 caccggaacc agtcgtcatt gttgaatgct tttctgttta gaatctgcca tccctggtat
    33241 ggtgagttgt cgagcagggt ctcgtcaatg tcaagcacca ctgcagccgg ctttttatga
    33301 cctgtcgggg cagtctcatc cggggtggag gggtctgccc cggaagaatt gttaactgac
    33361 agctccaggt tctccttcag ggcaatctcc gcattgcgga agcactggta gaaaagcgcc
    33421 ctcatctcgg ccgatttccg gaaccagagg gttgccagta tcaggtgatc ctgatcctcg
    33481 tgcgattcgg cggcgtgaag gttagcgaca gtctgtatgg gaagatccgc atcagccgga
    33541 ataggaaggt cagcagcaaa ctggctgggg gaattcgcaa cagccggaac gggaaggttc
    33601 cctgcagagg agacgggagg gttcgaggca gtcggtgtgc aaagctccat aacagccagg
    33661 gtgggaaaat acgcagtgtc cggggaggaa agatccgcag cagcctgagc gggaagattc
    33721 cctgcagacg gggcggaatg ctcctccgca ggagaagaag tctgcttcac cgtggcacac
    33781 gaagcaagca ttatactgag tgtaacaata atgagctctt tcattcgttt ttattctgtt
    33841 gataataaac taattgctcg gaaccggtac attttgtttg ttactaccta agtaacaaat
    33901 aattggccga taccgtttat attgtttgaa agatttttaa ctttaaccgt accataccaa
    33961 aaagtaagct gcaatgtcaa ccttaaagct gatgctgggg atgatcccct caacctccaa
    34021 gatcgaacag gaagagaagg ctctgattgc cgagtatgaa aagatgatcg cctttgccgg
    34081 ctcggaagcc ctggcaaaat acacccgtct tcatgaaagc gtcaattcag ccggttttca
    34141 acagaagaga aaggagatag aatcactcac atacaaaggc tctgaagagc atgccaggga
    34201 ggcagaattc ctgggcttgc agaaagcaaa agatattgtc tcttttttca aaaccgaagg
    34261 aagtgctctc ctgaagaatt ttctgtcgct cgacggctcg gcaaggatta aggagatcga
    34321 agaccttcag ctctttgtgc agtcacctga gttcaaacag aaggagagga tgaagcccat
    34381 caccttcaag cataccgaag agtatggcaa atggctggaa ttcaagaaac tgaaaggcga
    34441 cagggaggta aaaaagaacc tgaatcccga aaaggtgaat aggttcaccg aactgaaggc
    34501 actggttact tcagcagatt ttctgctgaa aaagaacatg aaaccgatca ctttcaaaga
    34561 tactcccgaa taccagaagc tccaggaact caaaacgaag aagagcgcca ctgacattgt
    34621 caacttctat aaattccgca aaacaaagga ttaccacaac tgggtccagg taaaggattc
    34681 cgcacggctg aagaggtatt atgaactcca ggaatatctg aaaacgaagg aattccatga
    34741 aaggaaggaa tatcttcttg acaagcgccg tttcgagaag agcgagatgt tcagagagct
    34801 taaggaatac gaaaccctga agaattcacc cgaaatcaag tggtacttca gcattaagga
    34861 ttcaaacaag ttcgatattc ttaaaaacag ggagctcacc ttcagcgacg agttcgaggg
    34921 cactgctcct gacagcagca aatggatcaa caggtattat ccgggcgaca agctgctgca
    34981 cgacagctac agcatatcca ctgatcttca tagttatacc ccctctgaca atatagaagt
    35041 acgtcacagc gtgcttcgca ttatcaccag gccgcaaaag acaaacggca aggcatggga
    35101 cccggcaaag ggattttaca caaaagagtt cggtttcact tccggtattg tcaacaccgg
    35161 cgcctcgttc aggcagaagt acggcacttt cacagccaag atcaaactca cggaccctaa
    35221 tgtccgcaac gcattctggc tgatcggtga ccgtatcact cctcatgtta acatctgcag
    35281 gtcgggcagc ggaaaggtct ggtttgatct tttcacctcg ccgggaagcc attacaaaaa
    35341 gagcatcgga tcaaggtatc tttctgattt ttacatctac accctcgatt ggacacccaa
    35401 tgccctggta tggaaaataa acggtgtgga ggtgatgcgt cagaccaccg gcgtgcccca
    35461 ggagccaatg ttcatcaacc ttgcaggagg cgttgacaaa ccaatcggcg ggatttcctc
    35521 catggagatt gactgggtca gggtgtacca acccaaataa ctgcatacca ggcatatatc
    35581 tgcaatggta ttatgtaacg ccgggtctga gacccggcgt ttcatttttt ttaccaatac
    35641 ctgtaacatt atgatgtttc aggccgtctt tatggtgtca gcatatttac ctgaatgacc
    35701 gtaaaagagt tcaacaacgc cgtcagggag ttcggagacc atgtctaccg gttcatcctc
    35761 cgcagcataa aagacagcca cagggctgac gatatcgtgc aggatacgta cgagaagctg
    35821 tggaactgtg tcactgagat cgagtacggc gccgtgaagt catggctctt caccacagcc
    35881 tacaacagga tgattgacgt gataaggaaa gactccaggc ttgttgatgt tgatcattac
    35941 gatgacagca cgctctatgc ggatgagaac agcagtgacc tgaacgaggt gctgcacagg
    36001 gcgctggaaa agttgccggc agtccagcgg tcggtaatac tgctgcgtga ctacgaagga
    36061 tacagctaca gggagatcgg tgaaataacc ggtttatcgg aagcacaggt caagacctat
    36121 attttcaggg gacgtgtggc tctgcgaaat tatcttgtaa agacatcagt atggcaataa
    36181 cggaagatat ggatttcccg cgactgacgc ctcctgatga caggatgcca tcgggtagca
    36241 gtctattccg cagtccggcg gaactgaccg atgagcagtt cgacctgctg gctgcagcat
    36301 gggctgaagg ggccctttca ggagagtcgc tggaggagct ggaatctgcc atggcagcca
    36361 gtgccgacag aagagatcgc gccgggagct tctgcagtat ccgccttgtg ccatgcgatg
    36421 agagctggcc aggaatgagg tctgccatca ggctttcacc ggtgcagaga acactccgcc
    36481 gttcagtgat tccgacactg ctggcagtgg cagcaatggt ttcaatgatc atatatggtc
    36541 cgacagtgtt aaaactgaaa accataaact caggaaaaac catcacccgg tcagccatga
    36601 cagtggcaga aattcaggta ccccccccga tagttgctcc tgaaaaaaaa cccgcaaccc
    36661 ataccccgtc ggctcaggta gcgcagacac cggttgcaga agagaacgcg ctgcctggtc
    36721 ttcttgcaga aaagaatgca atgcagaatc ttcccgcaga ggagaacgtc ttgcggggtc
    36781 atttcgcaga agttaatgca ctgcaaggtc atcccgctga agaaagcgca ctgcagggtc
    36841 atttcgcaga agcgaataca ccccaaggtt accccgcagc agagatacaa aattctgatg
    36901 agataacagg atttgtcagg gttcagcccc ttgcacttgc ccggggctat gcttcagtcc
    36961 catccgtggc tcctgaaata attccggcca tcacctcaat caatatcatg gatattcagc
    37021 ccgctcagac cttgcaggaa gaaaagaact ggataatccg cacaatttcc ttcctggcaa
    37081 gcgcagtggc gggagaagag aaagaaatcg acggttatat aatagcaaac ggctgcatca
    37141 acggtatcaa aaatgcattg ggatgggata tggaactcga ccttgtcagc aataaaagcg
    37201 gtgaaccagt ggctgtcacc ttcaggtctt cactactctc tttcataaaa cctttgaata
    37261 aaacaacccc ctgagtgtaa cttttcggca cccccggacg tcccggcagt aaataacaaa
    37321 caattcaaaa ccgtgaaacc cgcatctgtc aaacatacaa acacctgttt atatattttt
    37381 cagtcatgtg ggttccatca tgcctttaaa atcaaatata tatcatgatg aaaagagtat
    37441 tgatttcagc atttcttatc gtttcaatgc tcctcccggc cctggcacag gagaagggga
    37501 aggagcagaa gctgcaggaa cttgaacagc ttgaacagca ggtaaaacag gccacaattc
    37561 cggatacacc ggtttcagcg gcgggagaca cactggttgc ggaagcagag gatttactgg
    37621 ttgctgcagc agagcataca cccgtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37741 nnnnnnactg gttgcggaag cagaggattt actggttgct gcagcagagc atacacccgt
    37801 cacaacagcg gaagacacac tggttgcagc agcggaggat acgctggcag tgatcatggc
    37861 agaagatgaa gagatcgccg tggttgtcag cgacgatgcc gatgatacat caaccgtaag
    37921 gatcggtgag attgtgacca taaaaaatac tgatgatgag accattataa ggatcggcag
    37981 gaagggtgta agggtctatg acgaaggtga cagcaccgaa attgactttg tagataaaga
    38041 atacgggaaa aaagagcgcg gcaggacggg aaggcatttt tatggccatc tgggcggcat
    38101 tgaaatcggt tacaacaatt tctccacagc cacctggctg aaaaatgatg cacccattgc
    38161 aggctatctt gatctcaaca cttcaaaatc aattaacttt aatattgttt ctccccctgc
    38221 aagtatggga tttacgcgcc atttcggact ggtcacggct ctcggcatca gcttcaacaa
    38281 ttatcggttt gagggcaaca actcaattac agttgacgaa ggaggaatta tctccccgct
    38341 tttcccggca gatccggtca ggtatgataa gagcaagctt gcgacgatat acggaatcct
    38401 gcctgtagtg cttgaggtac agattccggt atcgcgggga agctcaatca acctgggtgc
    38461 cggcgtgatc ggcgccgtga agcttggttc atatacaaag gttgttcatt acaataacgg
    38521 caaacagaaa gaaaaaaatc atgaagactt caacctcaat atgctgaggt acggagtgac
    38581 cgtcagggcg ggatacgaga tggtgcaggt gtatggcacc tgctacctct caccgatgtt
    38641 cgaaaaggga aagggacccg agctctaccc gttcgaagtg ggcattgcgg tgactatcaa
    38701 tgactgacaa tacataacaa aacttctgaa tcagggggca tctggcagat gtcccctttt
    38761 ctgttattaa ttggttaaac cggaagcact tctttttccg gctgtataaa atcgggtgat
    38821 tttatgtaag tttggagcat tgatgcaaag ggcggtgagc cagaacagtg cccatatata
    38881 aaaacaaaaa acagattgaa atgagactac ttcttatcag caattcaacc aatgccggag
    38941 aggcatatct tgattacccg aaaaacaaca tcagggattt cctgggaagc cggaaggtaa
    39001 aggctctgtt tatcccgtat gccgcagtga cattttcata tgacgagtat gaaaagaagg
    39061 taaagaaccg tttcaacgag gtaggacatg atgtggtctc catacatcat ttcagtgatc
    39121 cggttaaggc aatcatggat gcaccggcta ttgttgtggg tggaggaaat acatggatgc
    39181 tgctgaagtg cctgagagac aataagctga ttgcccctgt caggaagaag gtgatgtcgg
    39241 gcacgccgta catcggatgg agtgcaggct caaacgtgac ctgccccaca ataatgacaa
    39301 ccaatgacat gccggtcact gcaccatcat ccttcagggc tttcgggctg attccgttcc
    39361 agatcaatcc ccattacctc gatgccaacc ctgccggtca tgccggcgag acccgcgagc
    39421 agaggataga ggagtttatt gaggtaaaca gggatttgta tgttgcaggg cttcgcgaag
    39481 gaacaatgtt cctcctggaa ggtaaaaagc tgtcgcttat cggacccaga aacgcacgca
    39541 tcttcaggta tggaagtgaa ccccgggagg tgaagccggg aagtgatctc tccttcctgc
    39601 tgaaaaaaaa caaacaataa tcatctgcac cgtcaggtgc catgagagtg ttccggaaaa
    39661 tagcgcttat tgccgccggg gtgctgctgc tgctcatcct ggtaccggga tcgtatgccc
    39721 tgctgaggca ggaacacctg acaaactcca ttgtcaggaa ggtcaatgag agtgtcaata
    39781 cgaagatttc gtacggcaac cttcacgtta caatcttcga atcattcccc aatattaccg
    39841 tcagattcaa cgacctcctg gttgaaccct ccccgttcta cgacaagaaa cagctgagtc
    39901 atgaggataa tgacacactc ctctatgctt cctcgctgtc actgacggtc tcaacgccgt
    39961 cactgctgac ctccacggtg gcagtaaagt cgatcactgc aaggaacggt gaagttaacc
    40021 tgttgactga caagaggggt gacatcaatt acgaggtgtt cagtgaaaag aagggagatg
    40081 gcaagaacct caggctgaag aatatcagcg ccattgacat caggacagtg tacaatgaca
    40141 taagcacggg aatgcgcatg gcaggaagaa taggcgatgc aaccctcggc ggggagctgt
    40201 tcggcacagg tatttacctt aacactgccc tgaatgcaga gattgactcc ataaacctgg
    40261 aaaaaataac aatgagggat atccctgtgg aagcgggcat cagactccgc aagtcggcaa
    40321 gctcgctttc agtggtaaag ggatctctca gcatagctga cctcaggttc gaaatgaccg
    40381 gaaatgtaaa ttactcatct tcaacactca acctcgttat tgaaggcagg aaaattaaca
    40441 ttgcatctgt aatctcgagg ctgcctgaca gatggaaaac tgtcaccggc aatttctccc
    40501 cctcgggcat cattgatctc aaatgcagta tcacaggtcc ttacggcgat gccggcaaac
    40561 cccacataga tctcacatac ggtctcgaaa gaggcaggat gagcctctcc ccctcaggct
    40621 tcagggtcaa caacctggag ttcagcgggg cgctcaccaa tggtgaccac aactcagcag
    40681 agacattccg cttcaccgtt gacaagctca ccgccaggta tggctccgca tcggtgaaag
    40741 gctcatttat gatgaacaat ctccggagac cccacataac ccttaacctt aacggtgacc
    40801 ttgactttga cgatctcgac aagattttca ggtcgggata cattaaagac cagaccggat
    40861 tactctccgg aagcataaga ctctccggaa tgcttcccga cagcatactc ccggcaacct
    40921 cccttgcgtt tctgaatccg gatatatcac tttttttcag ggagttcggt gcaacgctgg
    40981 ccggaagcag gttggccatc aaagatgtca ccggttctct gtcggtaaaa aatgacctga
    41041 ctgccgataa cctttccttt acttttcttg accagcgctt taccatcaat acggtcatga
    41101 aaaacttctt ctcctggatg gccggtggag gtcagagcct tgatataagc ggtgatatcc
    41161 atgttgacag gctggtcacc atggctctgt tacctgcaaa aagggaacag cctgacagta
    41221 ccggcaaacc ggttaatctt ttcccggcgg ctgtcagtgc caatattctt cttaaggctg
    41281 acagcttgat attcaatgat ttcagggctg ccaacttcag cagcagatta acatacaggc
    41341 cctatgtgat aacctttgat gaggttaatg ccatcggtct agatggagta cttgatggag
    41401 agctgatgct gggcaggcaa caggatggcg gatatattac aaaggccagc cttgatgtta
    41461 aaaatgttga tatcaaccag gcatttacct ccttcaataa cttcggccag aacttcatag
    41521 taagtgacaa cctgcgcggg aggctgacgg gcaaggtgac catgctctct ccccttgaca
    41581 gcaattacag gtttattcgc aaggcgattg tggctgagtc acatcttgtc atcaatgacg
    41641 gaaagctcat cagtttccct ccggcggaaa gcctttcggc atatcttgac ctcgatgagc
    41701 tgaagaatat cagtttctcg aaaatggaaa atgatatttt cataaattac ggaaccgttt
    41761 ccattccgaa aatgctgata aactcttcgg ccgtaaattt caccgtatac ggaacccacg
    41821 ggtttgaagg agattatatc tatcatgtcc ggctccttct ttcagaggta atgagccgga
    41881 aggcaaggga gcgcaaccgt aatgtcagtt cattcgggca ggtgaaggta gacggcagtg
    41941 gtaaggcaac agtgccgctt aagatagaat gtgtaaatgg caaaaccagt gttggttatg
    42001 actttggaca ggcacatgac aatataaaga atgacatcgc tgccgagaaa caggcactga
    42061 agggaatact caacgaggag tacggctggt actcgtctga cacaacccgg acaacgccgg
    42121 tggagagcaa gccaaaattc agcataacct gggaagaggg gaaggaacag cctgatgtgg
    42181 ctgaaccaca gaaggaagag gtgaaggaga gcccgtttaa aatccttctc aaaaagaaaa
    42241 gatagccaat gaacatctac cgaaaaaaac ttctctggaa ggtacttctt ctgatatttg
    42301 cggtggtgat cggcctcggg tcactcatct acacccatga tctggtagac agtctcaagg
    42361 aggaggagcg caagaaggcg gagcaatggg cggatgccac gcgcaggctt gtcacttctg
    42421 acgatttcga attcctgttc agcatcatag aagacaacac cactgttccg gtgatactca
    42481 ttgacgagga ggagagcatc atctcacaga gaaacctgcc ggcagcgttt gccaccgaca
    42541 ccctggcaat ggtccgcgag ctgaagcgta tggcaaaacg caatgaccct atctccattg
    42601 aactgcccaa cggcattgtc aaccatctct actaccgtga gagtacgctc ctgcgtcagc
    42661 tgagggtata tccgtatgtg cagctggcag ttatacttct gtttatcggg gttgcctatg
    42721 ttgccttcag cgcctcgcgt gtggctgagc agaaccaggt atgggtgggg ctctcaagag
    42781 agacagcgca tcagctggga acacctgcct catcactctc ggcatggagt gagataataa
    42841 gcagcaggta cccggggacc gatatagcgg aggagcttcc tgccgacgtt gaccggctga
    42901 gccgtgtagc cgagaggttt tccctcatag gatcaaaacc gaagctgatg cctgacaatc
    42961 tgccagccct tgccgaggcc acagcatctt acctgaggaa aagaattgcc tcaaccattg
    43021 acctgaaggt ggctgatgaa acaaccggat gcatggcagt acccttcaac gccgtgcttc
    43081 tggggtgggt catcgagaac ctggtaaaga actccgcgga tgccatgaag ggaacaggcc
    43141 gtatcaccgt caggctgagt gagaacagta ctgaagcctt tattgacgtc gaggataccg
    43201 ggaagggaat tccaagaaag gatttcaaga ctatttttaa acccggttac accaccaggg
    43261 agcggggatg gggcctcggc ctctccctct caaagaggat cgtcgaagag taccataagg
    43321 gaaaggtttt cgtgctccgc tccgagccgg gtcacggcag ctgcatgagg ataatactga
    43381 aaaaacaggt gtgatccggg agagaaatga ttaaccggaa cgatatagag ataatggccc
    43441 cggccggctc gtgggaggcg ctgagagctg cgatagcggg aggggctgac tccgtttatt
    43501 tcggcctcgg gaagctgaac atgagggccc gctcttcgca taacttcacc aatgacgatc
    43561 tgccagccat agttgcactc tgcagggagc acggcctcaa aagctacctc acgctgaaca
    43621 tcgtcgtcta tgacgaggaa ctgagtgaga tgaaagaggt gctggctgcc gcagcagcag
    43681 caggaataac tgcagtcata gccactgacc aggcagcaat ccttgccgcc agggagacgg
    43741 gaattgaggt ccatctctcc acccagctca atatttccaa tgccgggtca ttgaagtttt
    43801 attcacaatg ggctgatgtg gctgtccttt caagagagct taaccttcag caggtaaaga
    43861 aaatacataa tgccatcata agtgacgatc tacgtggccc cgggggcggg ctgatgaagc
    43921 tggaaatgtt cgtgcatggc gctctctgca tggcaatatc cgggaagtgc tatctcagcc
    43981 tgcacgagca caatgcctca gccaacaggg gcaactgcta ccagacatgc aggcgcggat
    44041 ataccgtcag cgacagggag acaggctatg agctggatat agataaccag tatatcatgt
    44101 cacccaagga cctgtgcaca atcagttttc ttgatcagat cattgacgcc ggggcaagag
    44161 tgctcaagat cgagggccgg gcacggccac ctgaatatgt gaagatagtc acccgttgct
    44221 acagcgaggc tgcaactgca gtggctgacg gaacattcgg acaggaaaag gttgaggggt
    44281 ggatgaggca gcttgtgacg gtattcaaca ggggtttctg ggatggctac tacctgggac
    44341 agaagctcgg ggaatggagc acttcctacg gctcggcagc cacaaaaaga aaaatctacc
    44401 tgggaaaggt gaccaactac tacgcaaaac cgggtgttgc cgagataaaa cttgagaccg
    44461 gagacctttc caccggtgac gaattgttaa tcaccggtcc gtcaaccggt atagtggagc
    44521 ttacagtgaa agaactgcgg gatgctttgg aaaaggattc ggcaaaagtc atgaaaggag
    44581 agctctgtgc agtgaaaacg gatggaattg taaggcggtc agacaaggtt ttcaaatggg
    44641 ttgatgcctc gtctctgaaa aacgagtgat ctcacctcaa cccttctgca gcctgcatca
    44701 ccagggcggt gtttacatcc tcgtcatcgg tttcagtgtt gatatagata acctgaccct
    44761 gtggtctctt tgacaggcca taatgatacg acttgtcata gtactcaaga atgactctta
    44821 tggccgtggg gtagtctccg cgtttaaggg catcaagggt ttcacgtgcc cggtctcctc
    44881 ccagccgttt tgatattttc accactgagg ccattatctc ctcaaccggg aatgaggtat
    44941 attcccttac cagcctgggc atacgtgttt cgatgctcat catcagggca atcacagggg
    45001 ctgattgaat ccggtcaaag aaagggtcgg gcatgaatac cgttccgata ttccggctct
    45061 catcctcaag ccataccggc gaatctgccc ggtgtgcatt gagatcatca taaagcatgt
    45121 ttgcaaaatg ttccgatgaa ggttgcagcg cctggcccag tgcgccaaag gcagagcccc
    45181 tgtgtgatgc aagtccctcg agatcggtaa cctgttcccc ggctgacttc atgcgtctca
    45241 gtatcccggt cttgccgctg cctgtaagcc ccccgaggat aatatagttc ctctcccttc
    45301 ccagatcacc gaggatatga ttcctgtatg ccttataacc tcccgtgagc accaggggct
    45361 caattcctcc cagggagaag agccatgcca ttgcctctga cctcatgcca ccccgccagc
    45421 agtaaaccag aagctttccg ccggcagcca gtttcagccc cgatttcagt ttgcccgaca
    45481 tcccgggtgc tgtaaggtca ataccccgca ccacggccct ggcgttgccc tctctcctgt
    45541 aaactgttcc cacctctgcc ctctgggcat catcaaagag tggaatgttt actgcaccgg
    45601 gaatatgtcc ggagacatat tcaccgggtg atcttacgtc tgcaagtggc agggaggctc
    45661 ttagttgcag gaattcatca gccggcagcg tattaagggt gaatgccatc gttatgaggg
    45721 cttgtggtac ctgtcaatca ccctcctgag ctcctcaaca gtgacaggct tggcaaggag
    45781 ttccttcatg ccgctcttct cgacattcag ccttgtttcg ggcatattgt ctgcggaaag
    45841 agctattatc tgagtggagg ggtttttact gagaatgatc cgggtggcct cgaaaccgtc
    45901 tgtctcaggc ataataaggt ccatcaggat gaggtcatat gatttcaccc cggccatatc
    45961 aatcgcttcg gcgccgcccg atgcaaactc ggcgctcact cccagtaccc tgagcaggga
    46021 gcccacaacc ttcctgttaa ggtaattgtc gtcaacgaca agaaccacag gaatatcagt
    46081 tttatcggaa tcgagtctgg ccaccggttt cttcaatgcc gggaagtgct ccctgaggac
    46141 attcatcaga tcctccgggg caaacggttt gaccagcaga tggtctatac ccaggtcaac
    46201 acacctggcc caatgcccct tcggatccct cgaggtaaac atcagaacta tatgttcgga
    46261 ggtaaggccc tcatccatga gtacttttgc tgcttcaaaa ccatcatttt ccggctcgtc
    46321 aaacaataca agcaaaatgt ttctgcccgt gccgctgttt aaccctgatc tgatctgtga
    46381 agccgtatgc ttctggaagc tggtgaccga gacaggcagt cccacgcggt gcatgatact
    46441 caggaagtca tcatcacggc cctgctggcc ggtaatcacc agtgtgcgta tgtcattgac
    46501 atcagcatac ataccaagat ctatattctt ggatatcttt tcattaaggt gaaccttaat
    46561 agtgaatata accctgaggc ccttctcctg gcccgaagca tccttcattg cagggctctc
    46621 tgcagtaagt tcgccgccca tcagttcggt gagctgcctt gcaagcaccg ggcccagctt
    46681 caggtcctca gcccagtcag accgttcgct gatagtggtg atgtaatcac cgaacagcct
    46741 ctttatctcg gccctggtat aggtattgcc gctatcagtg agggtgaact caagcatgaa
    46801 gctgtttcct tccgacttcc tgacacggca gctgagcttt atcagtcccg actgtgtgcc
    46861 ggcaaccgag ttggtcatga ggttgatgat cacctgtctc agcctgtagg gatcacctat
    46921 gatgttttca ggcactgcct gttctatctc agattcaatc ttgacgggga tctccgggtt
    46981 ccggcgcctg atcatgttga tacagtaagc cacctcatca cgcagcctga agggtatctc
    47041 gtcaagaatc atctttcctg tttccacctt tgaaacgtcg aagatatcat tgatgatggt
    47101 gagaagcagt tcggccgatc gcctgagcag tcttgccacc tcctttgatt cttccggaag
    47161 gtctgacctg ttgagcatct ccgtcattcc aagtatgccg ttgagcggtg tgcgtatctc
    47221 gaaactcatc cgcgccagga gttctgactt ggcgatattc gcgtcagcct catgccggcg
    47281 tgccagctca agtgaggtga tatcaatcag cgcctccagc gtgcactctt ctccgttata
    47341 tgtaacagga acagtgttga ggaaaatgat cttttcccct cccggggagg gaagcctgat
    47401 caccttcccc tctcccagct taccggcaat gtccatggca tacttattcc ccgagaggtc
    47461 aggcatgagc ttgccggtaa tcgctgtctc atcctcaaat gcaaagagac cggccgcctg
    47521 cctgttggca cgaagtatct ccattccctt gttatgaatg ataaacccga cgggcatccg
    47581 gtcaatgatg gaaagcaggc cggcttccga ggtggtcttc atctcaagtg cctttgactg
    47641 cttcctcgaa aaccttctga aaagcaatat gataaagagt atggtcagca tggtcagcag
    47701 ccccagaata acactgttcc tgacaatgta tttctgaaag aactccatat atgccgagaa
    47761 taccagacta aagtcaagac ccaggatgtt gacgggatag tatgacgaga gtatctcctc
    47821 tctctttcca ttctcaccct cagttctcat cgcatgtgtc agccttccgg aattgccctg
    47881 ttcaagtgcc cgggtaatgc ggtcaacatt ggtatagctc ctcttatccc cgccgttgtc
    47941 gaatattatc gtgccggaat cactgattac ccattgccac tggtactttt caaggttata
    48001 cttggcaaag atgccggaga aatagcgccg gaaatcaatg gtaatcacca ggttccccag
    48061 tacttcaccc tcatggagta ccggaaggta atatttgtac ctgtcacggt caggctcaag
    48121 cttatccatg gtgtgcatgg tgggtacagt ctgagccctg tatgatccgt caagccagga
    48181 atttcgctct tcatccttga agagtgtata tacatcgcca tttgtgttgt aaaacataag
    48241 cccgacgatg atatcactgt attttgagta atagagtttc atcttttccc ggacccgttc
    48301 attgaccccg ggatcggcaa agaaaagggt gatgtcatcg gaaaaaccga tctcggtcag
    48361 gtcgctgata atatacatgc tggtattgtc gatgtcagac ccagcaatca ttacctgcct
    48421 gtccaacaac ttgttcgaat attcaatctg gttcctgtag agactgatat agaacagtga
    48481 cgtgaccagc atcagcagaa tgaaagcaat atatgctgca agcgatgccc ttttcattgt
    48541 ctttgtgtac cggtatgata acgcttatct gtatttttca ttgtcctcca gcatcatact
    48601 tatgtagctt ctgtacctcc atagttctat gtcatcgcgg gccaccgctt ccctgaccat
    48661 gcagtcaggt tcgtcaaggt gaaggcagtt atagaacctg caccctgatg agatcctgaa
    48721 gatctccggg aaaaagtgat agatttcatt acggtcgaaa tctattactc caaaagcttt
    48781 tatgcccggt gtatcaatga cataaccgcc atcggcaaca gggtgcattt ccggataggt
    48841 ggttatgtgt ttaccctgtt cgtggtaatc ggaaatggag cctgttgtca gggaaagctt
    48901 cgggttgagc ctgttgagga gagtgctctt gcctactccc gagttgccgg agaagagggt
    48961 tatccggttt cgcagggcat cttcaaccct ggccaggctc ttgtcttcag tggccgagat
    49021 catcatcacg cggtagccga tcttttcgta gacatccctt atctggtaag cgcgggccat
    49081 ctcttcctct ccgtagaggt ccgtcttgtt tattaagata acagcaggta ttcggtaggc
    49141 ttccgccgtg gcgaggaatc tgtcgatgaa ctcaaccagt gtcactggca tcttcaccgt
    49201 tatgatgagg aatgcctggt caatattggc ggcaattatc tgcgactcgc gcgaaaggtt
    49261 cgtagccctg cggataatat agttgcatct gggcatgagc tctgtgatga ccccctcatc
    49321 agcgtcaaat ctcacatgat ctccaacggc aacaggattg gtggttcgtg ccccgctcac
    49381 cctgaagcgc ccccggagga ggcactcata tatgttcccc tcctcatcag ccacccggta
    49441 gcgcttcccg gtcgatttta tgacaatgcc ccttttcacc agcctgattt tgcagcaaaa
    49501 ataaaaaaag tgacaccacg attcggcggg catcactcat tttatacctg atttgtcaat
    49561 tgtcatcaat actctgaagc catcaggaaa aagggcttca tctcgaaatt ggaatagtaa
    49621 ggcctgagcc tttcagtatt gtagtattcc gcaactttaa caatcttctt ggaggaggtg
    49681 accacgtcaa tcggcacatt gtcatagcgc ccgttcttga gcactaccac cctgccgtga
    49741 atacccttca ttatgaggtc aagggccagg ttgccgaatg ccatcggaac tatcgagtca
    49801 actgcgtcag ggtcaccgcc cctgaccatg tagccaagct tctgattgat gatattgatg
    49861 gtttttccgt tgttgaattt ggctgaatgt tcctttatcg ctgcagaaat aaggtctccg
    49921 atgcctccca gcttggcgtg cccgtaggca tcatgctcat ggtcctggaa gatcatctca
    49981 ccccccttgt agagggtgcc ctcagagacc agaacaatcg aataccggct gggattggtg
    50041 ttgcgatcct ctacaagcag tcttgtaagc tgctcaatgt caaatttata ctcaggaatg
    50101 acgcacctgt ttgcagcgcc cgccatggtg ggcagcattg ccgtgaaacc ggcatacctt
    50161 ccgaacacct ccagtaccat gatacgctca tgtgaccctg ctgaagtgcg aagtgagttg
    50221 gtcatctgta tcgtacgggt gacgcatgtg ctgaacccga tgcagtagtc tgttcccgga
    50281 acgtcattgt ccatggtctt gggaatggca acaaccttca ctccctgctt gtagagatgc
    50341 acggcataac tgagggtgtc atctcctccg atggggatca ggtagtcaat acccagccat
    50401 tcgaggttct tcagcacctc cggggtcata tcattgattt catccttgta ggtatccttg
    50461 agatggtcgg gaacgtagtt ctttgccaca tggctcggcc ttgtgcgcga tgtgtgaaga
    50521 aaagtgccac ctgtccttcc tgtcctgttc accatgtcat cagtaagaac ctgataacac
    50581 aggctgttgt cagccttggg atccctgaca atctcaagga tgccacccca gcccctgcgc
    50641 agccctatta ctttataacc ttccctgtta gccctgatgg taaccgccct gatagcgggg
    50701 ttcagaccgg gaacgtctcc tcctcccgtc aggattccaa tcactccttt agttttcttc
    50761 tggtttgcca ttgtaaatga tgtgtttaaa actattaatg caagctataa aaatagaact
    50821 tttccaaatc atatcaaaaa agcaaactaa agctcgctgg atttttaaat atttttgaca
    50881 aaaaacagat gagcaaaaag ataaggcttc aggttacttc cgtgaaggct acagttgttc
    50941 tcagtcggtg cttctggcct gtgccgacgg aacccgcgag ctttcctgtg aagttatgct
    51001 tccgataata aactctttct ccggaagtat ccccgccctg gtggttacca cggttgctgt
    51061 tcccctgatc tctgtttcac cgtataccct gatgatgaag tttgcctcgg cggtagcccc
    51121 tcccggggta tagccaacag tggcagttat atagtcaggc ctccggttcc actcttcaaa
    51181 catcatcatg cttccgcgtc cgctaaccgg tggagccggt tcaataaccc tgctgcgggg
    51241 cgtgccggtg gtatcagcgg caaatttcac tcttacctca tcaggcctga ccatcttgac
    51301 aaggtgtgcc tgtctgaggg cggtggggag cttgccggca ttcctgaacc tgatggtcag
    51361 cttataatcg gccgaatctg cgcggtattt ttttattctt ttaaccgagt actcctccca
    51421 ttccagcctg ggcagatggc ttataagctc cttattaaag agcgcctggt ttgaggccca
    51481 tttttcaaga tgagcaggcg gtggattctg gctgaaaaac ttcggatgga atccgccggc
    51541 ctccacactg tcatagaccg gatggctcac aggcttccac tcatgaaaac cctcgccgtt
    51601 attctcctca tcatcccatg ccagcatgtc aatctggtcc ttgtcgccgt caccgtcata
    51661 atccttgtgt cttccgccgt tccacagctc atctccatac catattgcac catagtacca
    51721 gtatccgaag tcagggccat ggccgaagag cggtgacccg taaccgtagt catcatagac
    51781 gtctcctgct ctctcgtaac cggtgatgtt cctgcccagg tcatcaaaat agcggtacca
    51841 cttcaggtcc tcggggtaca ttctctcctc tgatggcgat gttgacggag cgcggaggtg
    51901 cataggcacg gtggtgtcca ttgagttgac aacatagata ttggggtgag tcatcaggaa
    51961 ggtaaagact gcccttgtct cgggttcgct gagcggatat gctcctgctc cccgctgggt
    52021 gaaacccctg cctgtctgtt ccctctcggg tctccagttc tccggatagt tgcggtgcag
    52081 gtcaagcccg cctatgccgt cttcattgat cctgccgtca ccatcattgt caattccctc
    52141 tgatgatgta gcatagattc cctcgccacg tggcactctc ttcatgatcc tacccgaggg
    52201 gtcatccggg tcaggaatca atgtgccgtt tttcctgtca ggccagcgca gcgtcaggat
    52261 aatgccatca ccgtcaaggt cctccggtgc gtcctcgtca aagagtccgt cgccgtcatt
    52321 gtcatcgggc cgcacggtac tgcggttgct ctgggcagtg ttgaggtaca gctcatgtcc
    52381 atcaggatta ttgactggcc tgaggtaaac ggtgaaatgg tcaagcaggt cggtaactga
    52441 cgggtcattg ccatatcctt ctatcaggtg cctggccagc cacagtactg actcagcact
    52501 ggttatctct ccgctgtgcc tgtttccctc aaagaaggcc ccgggcttgt cagtgtccga
    52561 accgttcttg ctgctggtga ttgtcatctg catgacaggc ctcccctcat agctgcggcc
    52621 tgtctcataa agggtgacta tttccggata atcctccgcc caccggttca tccagtggca
    52681 catcaccccg taggtatgat acctgtcaaa ggtgagagta tcaccggggg tgtattcaac
    52741 ttccgggtga tacacccggg gaaagaaact gacgccgtgc cgcggtccct ttacagtata
    52801 gagtgatgag tcaggcagga cgggctgctg cgcctcaacg gggaatatga gtgagacaaa
    52861 caggaacaga ataaaggctc cggtaatttt tttcatgtcg gacagaaagg ttaagattaa
    52921 acgctatcaa gataattatt tcggtgcaat gatcagcctc gccgtgaaat cacctccagc
    52981 ttgtttatgt caatgatctc tatattgcgg ccgttggccc tgattatacc atcacgcctg
    53041 aattccgaga aggtacgtat cacattggct gaactcatgc tgatgaggtc tgcaatctcc
    53101 ttccgtgaga ccggaagctc aaaaatcctc gaatgataga tctcatttga aaaatagagc
    53161 agcacaaaag ctgtgcggcc ggccaggttt ctcttcctga tctccagtcc gagagagatg
    53221 attccttccg cggtgcgggc ataagaagag atcaggttca gcgcaaattt cccgttccgg
    53281 agtatcagat ccttgatgaa ctctatatgc acgcagcaca ctatggaatc ttcaagggca
    53341 gagagcgagt agctgtacct ctcctcatgg aaggcgttgg tgttgcttac aaattcaaaa
    53401 ggcctggtaa tggtaatgat ctgctcgttg ccttcagacg tcactctgaa gagtttgaca
    53461 aggccgttct taagatattt gaagtatttt atcgattccc cctcgctgtg aatgatctcg
    53521 cccttgagat atgagaactc ctcctttgat tcacagacac gccttacatc atcctccgcc
    53581 aggttggtga atacaagatt cctcaactcg caggaaaggc atttgcaatt gtttttcggg
    53641 gtatttgctt cagcaaccat atattaccac aaatttaaga gaatccctga cattattcgt
    53701 tcacctttta tatttttgaa gcagtacttg ccagtttatt acagcagcag agatgattta
    53761 cggaactgat tatcacatgc ataccctcta cagtgacggg aagcgtgagc cagaggcata
    53821 tatagaagca gctataagag ccggactcag ggaggtgggc ttttctgaac acctgaaccc
    53881 gctgggaaga gacagggact ggtgtattga tcatgacagg cttccggctt acgccgaaca
    53941 cataatgagg cttaagacgg agtataagga gcaaattgct gtcaggctcg gcctcgagat
    54001 tgactatctg cccggcaccg aaaaggatac ggaaaggatt attgattcgt ttcccttcga
    54061 cttcataatc ggttcggtcc actatctggg agatgaaacc gtggacctcg gccctcagtt
    54121 ttatatcggg agagatgttg acaatatcta tgagaactat ttcaacctgg tctgcgaggc
    54181 tgcctccacg ggattctttg acatcatggg gcatcctgat ctcgtgcgca ttttccgctt
    54241 ttacccgcag gctgacatat ccctccttta cagcatgatg gcttcttcat tttccattca
    54301 tgatgttgcc tttgaactga ataccaacgg acgtaacaag ccgctccgtg atttttatcc
    54361 tgacaaacaa tacctgcatc tttttgcgca gcagggggtg gctgtatgcg tcaactctga
    54421 cgctcatttt cctgaacgtg tcggacaata tttccgcgaa gcctatgacc ttttgaagag
    54481 ggccggattc agggaaatgg ctgctttttc aggcagggaa cggttcatga taccgtttta
    54541 accattcatt catccgcctg ggagggcatc agcacgcaat acttcctgcc ggatcaaccg
    54601 ccggacatga gatgcccggc aagaatgcgt atcccgatta ctgccaggat aatacccccg
    54661 attatctcaa cctttgaacc aaggcgggtg ccggcatact tgccgatccg gatcgcagtc
    54721 atggatgcca ggaaggttac tgctcctatt atcagcccgg ccgtccagat cctgaccttc
    54781 agaaaggcaa ggctgatgcc cactgcaaag gcgtcaatgc ttgtgcccac cgccattgtc
    54841 agtaccaccg gcaaccggtt gatgtctatg ggctggcggt cggaactccc cttcagtccg
    54901 ccgataacca tccttatccc gagaaccatc agaagaataa aagccaccca gtgatcatac
    54961 ctcgagatgt agtctgaaac cgctgacccg aggaaaaaac ccagtaccgt cagtccgccc
    55021 tgaaacaggg caagaatgaa ggcaatgcgt accgcatttc tgaagtgaat cttcgactca
    55081 atcactccgc aggagagcga gacagcgaaa ctgtcaacgg aaaggcccag ggctattgct
    55141 gtgatggtaa tcaggtccat gagtgactga tgggcgcaaa aatagtaata tttgctattt
    55201 ttaccatcct gtttttatgg tatgaaaata gttgtacaga gagtgaaaag ggcttccgtg
    55261 acaattgacg ggaaggtaca ttcgtcaata gggaagggcc tgatggtgct tgccggattt
    55321 gaagatgcag acagtgctga ggatgtaaac tggatggccg ccaaggtggt gaacctccgg
    55381 ctgtttgatg acagcaacgg ggttatgaat ctctccgctg ttgagaccgg aaacgaaata
    55441 atgattgtca gccagttcac ccttcatgcc tcaaccagaa agggaaacag gccttcatat
    55501 atccgtgctg ccaggccgga aaaggcaatc ccgttgtatg aggagttcat atcgcgtaca
    55561 acaggccttc ttggcagaat tccggccacg ggggttttcg gtgcaatgat ggatattgaa
    55621 ctcgtcaacg acggccccgt tacaatcatt attgacagca aaaacaggga atgacagaaa
    55681 aggaacttaa gcgatcagta ctaactgtca cggtattcgc atccttcctg accccgttca
    55741 tgggctcggc agttaatctc gcccttccct caataggaaa agagctcagc cttaatgccg
    55801 ttacattaaa ctggatcata agcagttaca tgcttactac ctcaatattg ctgcttccgg
    55861 caggaagggc cggtgacata ctggggcgca ggaaggtgtt cactgcaggg atggcggtat
    55921 tcactctttc aatgatcctg ctgacattta ccccgggcat caggtggctg attggcagca
    55981 ggattctgca gggtatcggc ggggcaatga tgttcggaac caacatggcc attcttacat
    56041 cggtcttccc ccctggtgag aggggacggg caatgggaat caacgtgacg gcagtctata
    56101 tcgggctggc ctcggggccc ttcctgggcg gcctgcttac ccgttacccg ggatggagga
    56161 gtatatttgc cttactggtt cccatgggaa taataagcct tattctcatc cggacgaaga
    56221 tgaaacggga atgggctgaa gcaaagggag agagttttga ctggaccgga tcagtaatct
    56281 acggcatctc catggcagcg cttatgtacg gcttctccag gctgccctcc gcggagggat
    56341 gggcgataac tgcaaccgga gtggtgctga tgcctctctt catcatgtgg gagaaaaagg
    56401 ttgttcatcc gttgtttgac gttacgctga tctcacataa cagaatcttc gcattctcaa
    56461 gccttgcagc actgatacat tatgccgcca caagcgcaat cggcttcttc ctgagtcttt
    56521 tcctgcagta catcaaagat ctcggaccgc gcgaagcagg ctttgtgctg atgtcatggc
    56581 cacttaccat ggctctcttc tcgcctgcag ccgggaaact ctctgacaaa tacaacccgg
    56641 gcatgctggc ctcaaccggc atgggtatca cttctgccgg actcataatg ctctgtttcc
    56701 tgaatgagca gaccccggtg acatcaattg tggcaatact ggtaataatg ggagccggtt
    56761 tttcactctt ctcctcgcca aactcaaatg caatcatgag ctcggtggag aaaagacagc
    56821 tgggcaatgc ctcgggcatg ctcggaacga tgagaaacgt gggacagacc ctcagcatgg
    56881 caatagcact gatgctgctt accatctata tggggcagga aaaaatcaac ccgggcaact
    56941 atccgcagct tatgaattca atgaagaccg gattcataat tttctccatt ctctgctttg
    57001 caggaatatt cgcctcgctg gctagaaata aaagccttcg taaataatct gatttttatt
    57061 tgcttttcac taaaaacaac taaattgatc cccgtttgac ggctgtcaga ctgtctgttg
    57121 tttattatat ataaaactca ttatgagcaa gttatataaa aaactcttta ttaacgatta
    57181 ctcgcccgga gagatgggtg aatgtattgc cgaggccaaa aggatgcctg gcgctgagga
    57241 aagggctttg attctgaaag atattttttc atttatcgat ctcaccagcc tcaacacttc
    57301 tgacagtgat ggcaccatca ggagcttcgc tgaaaaaaca gccgggttca gctatcattt
    57361 caatgatatc agtaatgtgg cagccctatg tgtctaccct aattttgtaa aagtggcaaa
    57421 agaggggctt ggcggatgcg gcgtcaggct ggccgtggtg gcagccgcat tcccaaccgc
    57481 ccagactttc aaagaggtga agattctcga gacctccatg gcggtgagca tgggtgcaga
    57541 cgaaattgat gtggtcatcc ccgttggcac tttcctcaag ggagacattg acaccgtcat
    57601 cgatgaactg aaggccatca gggaagctgc aggaaacagc accctgaaag tgatcctcga
    57661 gacggggctg ctcaaagatg aacggaacat ctaccgggct tcaatcattg ccatggagtc
    57721 aggtgccgac tttgtcaaaa catcaacggg caagagcagc gtgtcagcca ctcccgaggc
    57781 agcatgggtg atctgccggg ccattcgcga ttattacaat gaaaccggca taatgattgg
    57841 attcaagcct gccggtggta ttgtcacccc caatgacgcc ttcatctatt acttcattgt
    57901 aaagaatatg cttggtgcaa aatggcttac accggagtat ttcagaatag gtgcctcccg
    57961 ccttgccaat catatactct cggagttcta ccccggcaag ccggcctatt attaacatgc
    58021 gcttatggcc agcggtccgg gcgataccat cagtgttgcc gctgataccc tcagcctgcc
    58081 tgtgcagcag acagagttca gcataggaca gattattccc ctggtctcac cggcggaaga
    58141 tattgccgtg aaggatgaga gagtagccgg gcttacggca gattatatct cgggactgaa
    58201 gcgcgttgac ggcataccga gagcttccgg gccggtgaac agtgacatga gcttcatcct
    58261 gctctcgctc tccttcctga ttattaccct gctgacacta ttcggacgca gaactgttat
    58321 cactggactc tcatcaatca gtttccggag gaatgaagag aacatccctg ttggcacgtc
    58381 agaggtgttt acctggcccc cgatattcag gaatattttt tccgtgctga atatcagcct
    58441 ttttgcatcc acgtcactgc ttgttacagg cctcgttgaa gatactctct tcaacggctc
    58501 caccggactg actgccatta ttgccggctc attccttgca tcccttttcc tgagacacct
    58561 ctcatgcata gttattgccg gattaaccgg tttcaaaagc cttttccgcg agtatatgaa
    58621 tgttatatac aattcatggt tcgcctgttc aattcttctt ttcatactta acggactaat
    58681 ccattttaca gccgttcaaa acccgcttcc gttcataatt gcagggttga ttattattgc
    58741 aatattcttg ctaatcagag tcttagtgtt gctttccatt tttatcgaca ggcatgtttc
    58801 tattttttat tacattttgt acctttgtgc ccttgaagtt ctgcccgtca tggttattct
    58861 caaactactc ggggtgttct gaccagcggc atgaacatga tggtgaacta aaaatctaag
    58921 gctttgaaaa taaggaaggt attagtgtcg cagccagagc caaccaatcc gaaatctcca
    58981 tatttcgagc tcgcaaaaaa atataacctg aaaattgatt tcaaacaatt tattcaggtt
    59041 gaaggcgtct ccgcgaagga tttccggcag cagaagatca atcttacgag cttctctgca
    59101 atcattttca cctcaaagat tgcaatagac cattatttca gaatatgtga agagttgcgg
    59161 attaccgttc ctgacaccat gaagtacttc tgcattaccg agaacgtggc attttacctg
    59221 cagaagtaca ttgtttaccg gaaaaggaaa gtattccatg gcaaagcctg ttttgaagac
    59281 ctcattgacg taataatcaa acataaggag gaaaaatttt tcgtccccct ttccgatacc
    59341 cacaaatcag atatacctga acttctcgca aagaataaga tcaaacatac catcggtaca
    59401 atgtaccgta ccataagcag ggacttcgat tgcaaggaac ttgattatga cctgctggtg
    59461 ttcttcagtc cttcggggat caaatcgctg aaggaaaact tcccgacatt tgtgcagggc
    59521 gatgtgaaaa ttgcagcctt tggccccacc actgccgagg cggtaaccaa cgagggtttc
    59581 aggcttgatc tgcaggcccc caatcccaag gccccgtcaa tgaccatggc tattgacaac
    59641 ttccttaagg agtataacaa gaattgcaaa tagcaccgga gagacataag atataaccgg
    59701 aagcatccat ccacccggat tgatgctttc tttttacaat gaaaaagcag aaggcatcat
    59761 tcagtaaaag cgagagactc tgcggagtca aagccgtcag tgaacttttt gcggaaggaa
    59821 aatccctgat tctgccgtca ctgagggtaa tctgccgttt tactcctgcc gttgaaggaa
    59881 ttcaccccgt gagagtgctc atcaccgtgc ccaaaaggca tttcaaaaaa gccgtcgacc
    59941 ggaatctgat gcggaggaga atcagggagg cctggcgtaa aaacaaggag ccccttgtaa
    60001 cccttatgaa acaagccgga cgccatgcag atatcgccct tatctggaca gatacctcgg
    60061 tcaggcccta cgaatttgct gagaagtgtg taacggacgc tgtttccaga gtatcaggac
    60121 ttaaatagta tctttgtgaa aatttcgaca atgaatagca aaaaaataaa aaagagagtg
    60181 ctggttccgg caattaccgt gactgctgtc attaccctcg gatttctgct tggcgggcag
    60241 gaaaaaaagg acttcaggct caccaaaaac cttgatatat acatctcact cttcagggag
    60301 ctgaatgcct tttatgtgga tgagattgac cctgagaaaa tggtgaccag gagcattaac
    60361 agcatgctcg caacccttga cccgtacacc gtctattacc ctgaagagga gaccgatgat
    60421 cttgatttca tgactaccgg caagtatggt ggttttggtt ccctcatcag aaaatcaggt
    60481 gattacatac tcgtaaccaa tgtatacaag ggtttccctg ctgataaaag cggtatcagg
    60541 ccgggagacc tgatgatcag cattgacgga acatcactga aaggcgtgtc ttccgaaaaa
    60601 gcctctgaca tgctcaaggg cgaacctggc accgaggcag agattgtcat aaggagaaac
    60661 ggccaggaga tcacaaaaaa gctcaggaga gaaagaatcg ccatctctgc cgttccctat
    60721 tacggaatgg ttgacgaaaa taccggctat atacggttta ccaacttcac ccagaactgc
    60781 atcgctgaag tccgggaggc gcttattgac ctcaaggata aacagggggc agaggattta
    60841 atcctcgatc tcagaagcaa tcccggagga ttggtaaatg aagccgtgga gattgtaaat
    60901 ctcttcgtca ggccgggtca ggaggtggta agcaccaggg gaagggcaaa gcaatatgat
    60961 gcagtattcc ggacaaacag gacgcccgtt gctcctgaca tgccggtggt tgtacttatc
    61021 aacaggggtt ctgcctctgc atctgagatt gttgccggag cgctccagga tcttgaccgc
    61081 ggtgttatag tcggtgaacg ctcctatgga aaaggactgg tacaggtggc ccgccctctc
    61141 agttacaagg cccaggtcaa gatgaccacg gcaaagtatt acatcccgag cgggagatgc
    61201 atccaggccg tcgacttttc caatcgcaat gaggacggga gtgtgggaac cattcctgat
    61261 tcgctcataa aaaccttcag taccagaagc ggcagaccag tcaaggacgg tggaggaata
    61321 atccccgatg tcaccgtgcc gtctgacatg ctcaacaggt ttgcaactga actctatatg
    61381 cagaacatga tattcgactt tgcaacacaa tattactggt cgaacccccg gcctcccgtg
    61441 cttgacagcc tgaagatcac ggagaacgac ctggatcagt tcacaaactt ccttgcagaa
    61501 aagaagtttt cataccgcac agactcggaa agtatccttg aggagctgac caaaacagcc
    61561 agggaagaag gtctgtacga ctcaaacagg gaggcaatgg agaaactgaa atcaggccta
    61621 agccacaccc tcgagaggga gatgacactc taccgtactg atgtgacgga actgatcgaa
    61681 tcggaactcg caggcagata tttctatgac tggggtatgg tggagtattc agtcacccgt
    61741 gacaaccaga taaagaaagc tatcgaaatt gccggcaaca gggagcagta cgcatcgctg
    61801 ctcaaggctc cgacaggagt atgaccatgt cactctgcca cttccgcagg agcagagtga
    61861 atggcatctc cggcaatatc aaagactggt tgccacgtga tgagtagccc ggccaccgag
    61921 gagagaaacc cggccactga gagcaggggc ccgggtactg aggagaggtg cctgatcatt
    61981 gaagagagga gcccggccac tgatgggaga agcctgatca gggatgagga gagaagcccg
    62041 gctactgagg aaaggagact gaccagagac ggaaacctgt ctcaatagag ttcctttttc
    62101 cacaccggga gccgctcctt gaccatttca agagtgtggc ggcacgcccg tgtcgcatga
    62161 tcgcgatgac cagaagtgac catgataaag agcgatgctt cgcctgccat gaccactccc
    62221 agcgagtgaa caatgaacac cgatcttaca tcgctgaaag ctgatctgac cgttgtaatc
    62281 acctcttcaa cctcctttac tgccatctct tcatatgcag aatactcgat agccgtgact
    62341 ctctttccgt ccgtctcatc atcacgtacc cttcctataa agattgaaac tgccccggcg
    62401 ccggcatcat cattgccaga ggccatcagt tcagaaataa gaacaggggt tataggcccc
    62461 tttatcagac aactgttttt catttgcaga ggtatatttt tcaacctccc tggaagggag
    62521 gcattattgc aatagtatca cttttctgta atccggaatc ccctttaagc agtgtccggt
    62581 tcaaagccat tctgaacggt atctgtttca gggcaggata cctggatatc agctggttgc
    62641 gcaatgaagc cgtgtcggcg gccataattg tctcttcgct tttcccggtg gcctcctgtg
    62701 ctgccccgaa atagagaacc cgtacttccg ccttcattgt cagtgtgcct ccagccagtt
    62761 ataacccgtt cccgtatcca catccagcgg tacgctcaac tctgctgctc ccttcatctc
    62821 ttccgtgaca atatcggcga gccttttcac ctggctctct tccacttcga agatcagttc
    62881 gtcatgtacc tgcaaaatca tcttcgcccc gggcatctcg cgctccagcc tggaggcgat
    62941 tctcaccatg gcaatcttga ttatgtcggc agcactgccc tgaatgggag cgttgatggc
    63001 gtttcgttcc gcgttacccc gcacaacctg gtttcttgag tgaatatcag gcaggtaacg
    63061 tctcctgccg aacattgttg tcacatagcc gagctccctg gccctgcgga tgctttcatc
    63121 catgtatgcc ctgacaccgg ggtatgagtt gaagtagccg tcaataagct ccctggcctc
    63181 cttccggcct attgtcagcc tctcagacag tccaaaggcc gagattccgt agatgatccc
    63241 gaagttggct gtcttggccc ttgcccgcat ctcacgggta acatccgcta ccggcacccc
    63301 gaagatcttt gaggcagtag cagcatgaat gtcctgcccg gagagaaaat cggaaatcat
    63361 tgcactatca ccgctcagat gagccataag tctcagctct atctgtgaat agtctgcact
    63421 gaggaagatc ttgccacttg ccggaataaa agccttgcgt atctcacgtc cttcagaatc
    63481 gcgtaccggg atattctgca ggttaggatt ggtggagctg agtctgcctg tggaggtaac
    63541 agcctggttg tatgtggtgt gaatccttcc ggtaacaggg tccacaagct gaggcagtgc
    63601 ctccacatag gtggagagca gcttcttcag cccgcggtag tcaagtaccc tttcaacgat
    63661 ggggtgcctg cctgtaagac gctgcagcac ctcctcatca gtacggtact gctttgtctt
    63721 tgtcagcctg gcattggcgt ctaggcgcag tctgacaaac aggatatcgc ccagttgttt
    63781 cggtgatgaa atattaaact cctgtccggc ataagtgtag atctcctgct ccagcctgat
    63841 gagattctca cgaagagtta cggcataatt tgtcagcacg ctttcatcga gcagcacacc
    63901 ggtacgctcc atggctgtca gtaccctgat cagcggcatt tcaacatctg ctgccagcgc
    63961 gtcaagttca ttttgtatga gcatcggctc aaagagcttc atcagctgcc atgtcacatc
    64021 tgcatcttca gctgcatact ctttgactct ctccctgtca atctccctca tggttttctg
    64081 ccgcgggccc cgttctccta taagctcctc gatatgaaca ggtttatatc caaggtactg
    64141 ttctgccaac aggtccatgt tgtgacgctt gtcaggttcc agaaggtagt gggcaatcat
    64201 ggtgtcaaac aggggccccc tgacactgat tccgtatccc gcaagcacct gcatgtcgaa
    64261 cttgagattc tgccctgtct tcctgatctt ttcattctcc atgacaggcc tgagcggttc
    64321 aaggagtttc agggccgcat ccttctccgg cgggagccag agcatggtac ccgaaccctt
    64381 ctcccatgag aaggctattg ataccagctg tgcattgagc gggtcaagcg agtttgtctc
    64441 ggtgtcaaaa cagaaatcac tcagttttga taactctgaa gccagcatgg ccgcctcctt
    64501 ctccgtttca atcagggtat acctgtgagg aacactattt atatccgctg ctgttgtttt
    64561 tccggcgggt gataataaat caccgaagag tgatccctga gactctcctg gcttcggcat
    64621 tgccgttgcg ggggattctt cctgtgcggc gctgtcgccg gcaggctggg agagcagatc
    64681 tgccggggca gcctgcttaa caccgggagc gggaagcttt gcagccattg ttctgaactc
    64741 gagctcctcg taaagcttcc tgagtgcctc cgtatcggcc tcctgcctgt gaaatatggt
    64801 atgccggagc tcaaccggca cattctgctc tatggtagca agctgtttgg atagtaatat
    64861 ctgctccctg ttctgctcta taatatcctt ttggcggccc tggagcgccg atgtgttctc
    64921 aaggaggctc tccactgccc cgtgttcatt gatgagctta caggctgttt ttgggcctat
    64981 tccgggggcc ccggggatat tgtctgaggt gtcacccatc agcccgagca ggtcagtaat
    65041 gtgttccggc ggcacaccaa actcccgtac aatctcgtcc ctgccccata cctctgcctc
    65101 acctcccgaa cggcccggcc tgtacatgac cacattgtcg gtaaccagct gcgcaaaatc
    65161 cttgtcgggt gtcatcatga atgccctgta accctgttcc gcagctttgc gtgccagtgt
    65221 tcctatcaca tcatcagcct cgtagcccgg aacctcaatg accggaatgc ggaaggcctc
    65281 aaggagacgc ttgatatagg gaacggcaat cctgatgtct tcaggagtgg gatcacggtt
    65341 cgccttgtag gcgggatata tctcgttcct gaatgttggt ccgggaggat caaagacaac
    65401 tgctatatgc gagggtttct gtttggaaag aagatcttca agcgtaagca ggaagccgaa
    65461 gatggccgat gtattcatcc ccccggatgt gacacgggga tttcttatga aggcataata
    65521 tgatctgaaa atcaatgcat atgcatcgag cagaaatagc ctcctgtcgt tgtctgtctc
    65581 tttcacctgt tgtcggatgc atgccactcc gcgcacgacg tggctgtttc aaataatctg
    65641 atgctgtgga gatatgttcc ttccggcatc ctgctgccca gcgtggctgc aatgtcggct
    65701 accaggttct cacaggtagg ctggtatggc gaaactatga cattgccgaa gtgctgacgg
    65761 tatgtctcaa cagtggtagc gggagccagg ccggaaacca tcagggcatg gtcaaacctg
    65821 tcaacaatgc tctcatggac aatcttcctg agatctccga aatcaatcac catcccgttc
    65881 ccgggatggt caggattatt taccggctcc ccggcaacag tcacgtaaag aatatatgag
    65941 tgaccatgaa cgttccggca gggaccgtca tagttccaga gggcatgtgc catctcaaag
    66001 cgaaattcct ttgttacccg tatcagggcc atttgtgcaa ctgtttattc catgcaaaaa
    66061 taatctattt ggaccgattt acgtttacgg tgatgtttaa ctatgatatt tgcatgttta
    66121 tatgtcatgc atatcaatta aaaagtatct ttgcgcccac tatattcata gccaatgact
    66181 aataacgaaa tattgacaat cccctctctt atgggtgaga gcttcaggaa gcacggaaac
    66241 aaccaggcaa tgggatttgt aggagaggat gccattacat ataaccagct cagggaaaga
    66301 acacggtcag tgatgaggct ccttgaagat ctccggattg aacagggtga caaggtggcc
    66361 atactgagca cgggcatggc caactggggt gtggcttacc tggccataac ctcaatggga
    66421 gcggtagccg taccgattct tccggacttc actccgttgg aaattgaaaa tattctgaac
    66481 cactccggga caaaggccct gttcgtttcg ggcaacctgg cccataaaat ttcaggatac
    66541 gggccggaaa cgctcgaaac ggtgatagca attgatgatt tccggatcac ttcaggaaat
    66601 gaccagatat ctttcgttgc cgggtctgaa ccgtcaggaa catacaatgt ggcagaggaa
    66661 gatctggctg tgattatcta tacgtcgggc acaacaggca agtcaaaggg agtaatgctg
    66721 acacacagga atatctgctt tactgccagg cagattatgg gaatacaata tgtggttgag
    66781 agtgatgttt tcctttcagt cctgcctctg tcacatacct atgagcagac tctcgggctt
    66841 gttgttccgt tattcaacgg tgccgtaata tggtacctgc gcaagccccc tacaccgtcc
    66901 gttcttcttc ctgcccttca gcagatcagg cccacaacta tgcttaccgt tccgctgata
    66961 atggaaaaaa tctatttcaa caaggttgtc cccacattcc agaagaacaa ggttttagca
    67021 tttctttacg gcacccggct gtttcgccct cttttcaaca ggattgcggg aaagaaaatc
    67081 tacagggcat ttggcggcag actgcggttc ttcggcatag gcggcgcaaa gctcaatccg
    67141 acggtggagc agtttctcct tgatgccaga ttcccgtatg caatcggata tggtcttacc
    67201 gagacatcac cgctgcttgc cggaacaaag gttggcacgt gcaggcttgg ctcaaccggt
    67261 cccgccatgc cgggtattac acttaaaatc aatgatcccg atccggtcac cggcgaaggt
    67321 gaaatatggg caaagggcga taatgtgatg aaaggatact accgtgaacc ggctattact
    67381 gccgaggtgc tgacacctga cggatggttc aaaacaggtg atcttggtgt ggttgataag
    67441 aataacttcc tgagcatcaa gggaagactc aagaatatga ttgttggctc aagcggagag
    67501 aatatctacc cggaagagat cgaatcagta atcaatgatt tccgccatgt cattgaatca
    67561 cttgttatcc agaaaaaagg taaactggtg gccatggtct acctgaacat cgaggagttg
    67621 caggctaaat atgcacagat gaagaaggat tttaacaagc aggcagagat catcaatgaa
    67681 tacctcgaag aactgcggag gtatgtcaat gcgcgggtga acaggtacag ccagctgcag
    67741 ctggtggttt accagccgga accatttcag aagactgcaa cgctgaagat caagaggttc
    67801 ctttattact gatcgcgttt accgggcaga tgaccggttg atttaccatc acaattcaaa
    67861 tgatattaat gccgtttttt tgctaatttg cggccaaacc ggaacgtgat gtcgaacagg
    67921 acaaaggcac tgcttctcat ttttactatt ctgattgcag atcagattgt aaagatcatt
    67981 gtcaaaacca atatggttat cggcgaggaa atacctgttc tgggaagctg gttcaggata
    68041 cattacctcg agaataacgg gatggcattt ggcctggagt ggggtggcaa agccggaaag
    68101 attgccctct ctctcttccg tatggcagcc attgccggca tagcctggta tcttgacagc
    68161 ataataaaaa agaatgccca taccggcctg atcctttcga taagtgcaat tattgcggga
    68221 gcggccggga acctcattga cagtgcgttt tacggtatga tcttcagcga aagctggcat
    68281 acaccggcgg tattgtttcc ggaaggaggc ggatattcat cattcatgct tggcagggtg
    68341 gttgacatgc tctactttcc gataattgac acgcactggc cagactggtc acccttcagg
    68401 ccgggacaga catttatttt cttcagaccc gtgttcaata ttgctgacgc ctctatcacc
    68461 acaggggttc tggctatact tgtcttccag aaaagattct ttggagagaa taaatcgtga
    68521 acggtattta aggctgggta tcatcaaacc ttgctgcctg aacaatttgt tccgcatttt
    68581 atcgtaattt tggtaatcat agcttcagcc gggaatggca agcctatcag acataaagag
    68641 accagtagag acagagatgc gtgagtttga atcttatttc agacgcaccc tgcagagcga
    68701 tattcctttc tttcatgtca tccttgataa tctgctgcgc cgcaaaggca agcagatgag
    68761 gccactgctg gtttttctaa ccgccaggct gaacgggaca atcaatgaga ccacatacgt
    68821 tgcagccacc ttcatagagc tgctccacac agcgacacta ttgcacgatg atgtggttga
    68881 tgaagcctct gaaaggcgcg gattcccctc aatcaatgta atgtacaatt ccaagattgc
    68941 agtgctggcc ggcgactatc tcctggccca gggcctgctg atcagtgtgg aaaagcagag
    69001 tttcaacatg ctgggcattg tttctgaggc agtgcgtgag atggtgaagg gagaacttgt
    69061 tcagttgcaa aagacccgta agcttgacat aagtgaagat gactatttcc ggataatcaa
    69121 aatgaagacg gcagcactca tctcagcatg caccgcctgc ggaaccagct cggtcactga
    69181 tgatccggag accatcgcac gaatgaagga gatgggacag aatatcggca tcgccttcca
    69241 gataaaggat gacctgctcg attataccgg caacgggctg acaggcaagc gtgacggcaa
    69301 tgatatcaag gaaaagaaga taaccctgcc actaatatat gctcttcgcg aagccggacc
    69361 cgtcgagcgc cggcatatat tcaggctcct gcgaaagaag aaaaagagca gtaaagaaat
    69421 cagggaggta atcgggttcg tgaaaaaaca tggaggactc gaatatgccg agagggtcat
    69481 gaaggagtac tctgaccggg caatccggat acttgagaag tatagtgatt ctgatacaaa
    69541 gtcagcgttg actgagttta taatattttc tacattaaga agaaaataga ggatgcaaac
    69601 tgcattctca cgcccggaaa atatgctata acaggagtac cgggaaatca gaaaacaaaa
    69661 atttcaaatc aaatcctact gccgggtaac tctatataac aggagtgccg ggaaatcaga
    69721 atcaatacgg aaaggtcaat aacacgagtc atggaagatc tggaacaata cagaaaagtc
    69781 atgttccgga atctgttcaa aaggataact aacccaatac ccccggtaac cagagggcat
    69841 cgattttttt caccactttc ttaagaaaat atccggggga gtgcccggtt gaggggaaat
    69901 attcaattct cgtgaagtta tcagtgcagg taaggtgctc ttatatgctg tatacctgcg
    69961 tcttatgatc aacatgtcaa tgttttgttc cacgtgaaaa aaatcaattt ctgagcgcag
    70021 cggccaaaag tgaggctttt ttggcaccaa ctatcttttc aagctcttcc acggttgctt
    70081 cggagagttt tttgaccgaa ccaaattttt tcagaagccg ctcctttgtc ttctccccta
    70141 taccgggaat ttcatcgaga attgaacggt tcatgacgga actacgcttt cccctgtgga
    70201 aatttatacc gaaacggtgg gcttcgtttc tcaggtgctg gataattttc agggtgatac
    70261 tgttcttatc gaggtaaagc ggaacggtgt cacccgggaa gtatatctct tcaagtttct
    70321 tggctattcc tatcaccgtc agccggcccc tgatgcccaa tgcgtcaaga ctcttcacgg
    70381 ctgacgacag ttgccctttg ccgccgtcaa tcaccaccag ttgcgggagt ggcttctgct
    70441 catcaagcat cctcctgtag cggcggtaaa tgacctcttc cattgaggca aaatcatcag
    70501 ggcccgtcac cgtttttatg ttgaaatgcc gatactccct gttgctgggc ctgccgttcc
    70561 ggaatacaac acaggctgca acagctgatt cgccctgtat gttggagttg tcaaaacatt
    70621 cgatatgcag gggaagctcc ggcatgtgaa gatccttctt catcttttca aggttttttt
    70681 ctgtctgctt ttcctttgac cggtctgccc ttttcttttg tctttccagc ttgaaaaaca
    70741 cagcgttgcg gttagccagc tcaagcagtt tatacctgtc gccccgctgt ggaaccgtgt
    70801 attttacctt atcaagcttt atatcaggca taaaaggaac gattacttcc tgagaatcac
    70861 ttgatacccg ctgtcttatc tctgtcacag cggttgagag aagcgactcc ttttcctctt
    70921 ccatcctcat tttcagttca acagaccatg actggacaat ggcgccgcta acaaccttta
    70981 tgtaactgat atagattgat ccctcttccg cagagcatcc aaatacgtca acattcctta
    71041 cagaagtgtt gacaaccact gatttgctgc ggtatttcga gagcaattca agtttctcct
    71101 taatcttctg tgcctcttca aatttcagat cttttgcata ctgccgcatc agtgattcaa
    71161 ggtatgccgt tacgccggaa aggtcaccct tgattatttc ttttatcctg tctgtctgga
    71221 ggttatactt ttcctcactg ataaggccga tacatggtgc ttcgcagttt ccaatatggt
    71281 attccaggca ggtcttgaat ttgccttctg aaactgacct tgtgttgagg ggcagtgaac
    71341 aggtgcgtac gtgaaacagg tgccggacca gctccaggag ggttttgact gccggaacgg
    71401 aggtataggg accgaaatat gctgatccgt cagctaccag tctgcgggtc atgaaaactc
    71461 tcggaaagtg ctcattttta acgcatatcc atggatatgt cttgtcatcc ttgagcagga
    71521 tattatatct cggctgatgt tttttaatca ggctgttttc aagcagcagc gcttccgatt
    71581 cattatccac tacaatatgc cggaggtcgg cagccctgga gagcattacg cgggttttgc
    71641 ccgactggtt tcctgtgaag taggagccaa cgcgtttgcg caggcttcgg gctttgccaa
    71701 catacagaat ggttccggaa gcgtcaatga actgatatac gcccggttta tcgggcagtg
    71761 cagataccgt tgattttaag gcctctactg ctgacatatc acttactcat ttctgccgcc
    71821 atgctgactg tctctgtctg aaggccagga caggattggg tagtgggcaa agttagaaat
    71881 tatgccctga caaatggctg aaaagtgata aggagataga ttgattttac tcaattatct
    71941 cattaaccgg aacttatatt tgtacagaca gtgtgcctgc agttaaggtt aattaatcct
    72001 gtacttgctg tagggatcag acacgaaaag tggcatatgt ttgaatgtca ggacattaaa
    72061 taatccctta tgactcattt tcatcttcgg aatgacgagc agcgccatga aagagagtgt
    72121 catgaaaggt gcatccagcc tgcttcccag gctcctgacc ctttcagaga gtttttcata
    72181 gcgtgatgcc atttcggtta cgggcaggtc tgacattata ccggcaaccg gcagttcaag
    72241 tatttccgtt ccctcttccg atgcaactgc cataccgcct ccggcatcca caatcatgtt
    72301 tattgcattg acaatatcgc ggtcatttgt gccaacagca atgatattat ggctgtcgtg
    72361 ggcaactgat gttgccattg caccctgttt cattccgaac cccctgataa aggccatggc
    72421 cggaggcttg tcattgtatc tctccttgac aactattttc aatatgtcat cttccggcct
    72481 ggggttaaca aactcctcct cccctgcctg tacaatatca ctctctgttg ttatctcccc
    72541 gtttctggca acgatcagat taatctttct gccctcgttg attaccctga tctcatcggg
    72601 cagaagccgt gtacatttga atttgtttat tttaatgatt tctcccggtg agaagagaac
    72661 tttctcgccg tcaaacacgc attttccgtc tatccaggtc tgaagaacat tcatggtttt
    72721 cggatcatca acaataataa aatcggccgg atctccgggt ctgagtgttc cggcattgat
    72781 cctgtagtgt tcataggtat ttagcgatgc aaccctgatg acatcaaata tattatagtc
    72841 cattgaaatc agccgtgcaa ccagtttgtt aatatgtccg cgcagcagcg cctcggggtg
    72901 aaaatcatca gtgcacagca tcaccctttc aggataatag ctgatcaatg gtgcaagtac
    72961 ttcaaggttt ctggcagcgc ttccctctcg tatcagtatt ttcattccgc ctgatatttt
    73021 ttcaagggcc tcttcgagtg aggagcattc atggtcggta gtgattcctg cagaaatata
    73081 ttttttcaat tcttctcctg tcaggcctgg tgcatgtccg tcgattggta taccggcatt
    73141 acgtgcagct tccagcttaa ggtgaacttc agcatcatta tatataactc ccgggaagtt
    73201 catcatttca gccaggaatt ttacctttcc ctcttcgatc agttccttta tctctgctga
    73261 tccgattttt gcccctgcgc tttccagtga agtagcaggt acacatgatg gggctccgaa
    73321 gattatttta accggtacat cttccgcatt atccatcatg aaatccactc cttccttccc
    73381 aagcacgttt gctatctcat gaggatccgt tacaaccgca actgtcccgt gctttacggc
    73441 agcctctgcg aaatgagatg gcacaagcat gctgctctct atatgaacat gcgcatctgc
    73501 cagtcctggc atgatgaaat gatcaggact ctcttcggtc tcagttacag actcaatttt
    73561 accggcttta atagtgataa tcccgggata aatgcgcttt ttatgcacat caattatttt
    73621 tcctgcgata ttcataatgt ataaataaat aatgttccac gtggaacatt gattattatc
    73681 tgccaagata cattattaaa atagaaacat cagatgaaga tacaccactg attctggaag
    73741 cctgtccgat cgtcgcaggc tttattcttg atagtttctc tcttccttcg gtagatatcg
    73801 aaatcagctc atcataatct atttcctctg gtatcttcaa tctttcaaat ttctttatct
    73861 tttctgctac gcccttctct ctctctagat aaccggaata cttgatcttt atttcagctg
    73921 cctgaataat ctcacttttt ctttcagatt ttccgtaact cagcctctcc tccacatgca
    73981 ttgtgtcaag aagctcctcc agataaatct gtggccttgt tgcaatacct attgctttaa
    74041 ttttctgatt aatttcagtt gtattctttt ccttaaggaa tgtattgatc tcttcaggtg
    74101 aaacagtgtg atttcttaga aatgtaacca gctcatcaac catcctgtac ttctcttcaa
    74161 ctcttctaat cctgtcctta ctggcagtcc ccagttcagc agcctttggt gtcagacgct
    74221 cgtctgcatt gtcctgcctc aacagaatcc tgtattcagc cctggatgta aacattctgt
    74281 aaggttcatc aatacccttt gtaaccagat catctatcag aactccaata taggcttcat
    74341 ccctgttgag aatgaaaggc tcctttccct gcattctcct gacagcgtta attccggcca
    74401 tcagtccctg ggccgcagcc tcttcatatc ctgtggtacc gttaatctgt cctgcaaaga
    74461 aaagcccgcc aatcctcttt gtctccagag atagttttaa ctgggttggc tggaagaaat
    74521 catattcaat tgcatacccg ggtctgtaaa ttacgacgtt ctcaagccca cgtatcttcc
    74581 ttagggcaga aagctgtgtt tccagcttca aactgctggc aaaaccattt atataatact
    74641 cattcgtctc ccatccttca ggctcaagaa acagttgatg tgtctccttc ccggcaaaag
    74701 taacaatctt atcctctatg ctcggacagt atctcggtcc acttcctttt atccttcccg
    74761 tatatagtgg agaatatttc aaaccttcac gaatgattcc atgagcaatc tcattcgtgt
    74821 gtgttatgta gcaacttttt tgattcttta tgtcaaccct gttgtgcaga tatgagaagc
    74881 atcgtccctc attgtcgccc ttttgttcaa ccatagctga gaagttaaca cttcttccgt
    74941 caacccgtgc gcttgttccg gttttcagtc tttcaaccct gaatccaagc tccctgagct
    75001 gttcactcag tccgcgtgaa tccgggtcgc cagaccttcc tccggacatt gtgttgaagc
    75061 caacatgcat cagcccgtta aggaatgttc catttgtgag tataactgca tctgcatgca
    75121 attctgttcc cagggtggtt gttatacccc ttatatttcc atcttcaatt ataatgctgt
    75181 cagctacctc ctgccatatt gcaagattat cggtcttctc caggattttt ctccactcca
    75241 tgctaaagag catcctgtca cactgtgcac gcggactcca catggcagga cccttgcttc
    75301 tgttcagcat cctgtactga atcattgacc tgtctgtcac aattgcagag cagcccccaa
    75361 gcgcatctat ttctctgatt atctgtccct tagctatgcc gcccattgcc ggattgcaag
    75421 acatccgggc cagttttgtc atatccattg taacaagcag cgtctttgca cccataacag
    75481 cagcaatatg tgcagcctca cagccggcat gaccgccgcc tacaacaatt atatcataat
    75541 gtaaaatcat tcggcatatg ataaggccag atatatatct tccatctcag acatcttttt
    75601 cttcccctcg tcttctctgt catcatatcc gcataaatga agcaatccat gaaaaattac
    75661 ccttcttatt tctgatctga agggtacgtt gaattttttt gcattttgcc tgactctgtc
    75721 aacactgata taaatttctc cgttaacagt atttccctta ttgtaattga aggctatcac
    75781 atcagtgtaa taatcatgac caagaaactc tttgtttatc ccatgcagtt tctcatcggt
    75841 tgtcatgatt atgtcaatga tacctgttct gagcctggca tctgaaacaa tgcgatttag
    75901 aatcttttta attattctgc tttcacgcag tctgaatttt tcgatatcgt aatggaagct
    75961 tactctcacg gattcagtat ttaaacttca tccttacctg attacccgaa gggttgtatt
    76021 ctatattgtc ggcaaggctc ctcataataa atacccctcg tccgtgaagg ttctcaatat
    76081 ttgcagggtc ggtaggatct ggcagatgat tgtaatcaaa tccctctccc tgatctgtaa
    76141 cggttacttc gaaaacagta ccgtcagcta taaaatcaac ttttactgtt ttagtctcaa
    76201 tacccttgtt tccatgtaat atagcattgt tcacagcctc aaccgttgat atcagtattt
    76261 tgccgtaaac ctcgtcagag ataccaagct tctttgacaa tttatctacc tgcgcttcta
    76321 ccgcgcgtaa attttcaatg tttgaattaa tagtaatgct ttccattcca gaatacctat
    76381 cactattttt taactgcaag ggtatttatt tgttgcaaaa tttagtcatt tttttgtaag
    76441 ccactgttcc gggtcaagct tttttttctc ctcgtaaacc ataaacttta gttttgcctt
    76501 accaccgtcg ccattatcca caaagacatc acctataatc tgtcctgtca gaaccgcatc
    76561 tccctcctta actacaacat ttacaagatt ctgatataca gtaaggtatt taccatgcct
    76621 aataattatt gccatatttg cccctgaaat ggcaaacact ccccctacca tacccttgaa
    76681 tacagatttt gcctttgttg gaccgttcgc cgtgatctcg atcccgatat tatcttcgat
    76741 tacattctta agaaccggat gctgatggct tccgaaatga cttgttataa taccctgttc
    76801 taccggccat gggattcttc ccctgttttc tccgaatgat tcacttatca ccttcatttc
    76861 tgcactcatc ggtgctgcac cgcctttctt cctctcggcc tctataagct tctctatctc
    76921 cttctcaatc tgtctggcta ttcttctctt ctcattaagc tcctgattca actgcttttc
    76981 cttcctgctc agactgctca ccagtttctc cttccttccc tgctcctgct taagaatttg
    77041 cctttgcctt acttctttgt tcttaagttc attgacgttc tttctgtcct tttccagtct
    77101 ctcctttgtc gcctgcagct gtgagtaaag ggtctctatt gtctcagcct ggtttcttct
    77161 gtatctggca acctgctgta tatatttcaa cctcttgtat ccctggttaa agtcagttgc
    77221 cgagagaatg aaagccagtg caggcattcc cttcgtagct ttatatgcac tcaatattga
    77281 tctttcataa tcctttttgt gatcattcag atcctgctcc agcatctcaa ttgccagcgc
    77341 atttaaatca atcctgtact ccagaagatt tatctcctgt ccgaactccg caatcagttt
    77401 ttcacgcaga ttcagtttcc ttccaataac ccttaattca ttcaggctct cgtttttctg
    77461 tgattcagtt gttttcagca gcttgtcgac atactcgatc tcctggagat tcttcttcct
    77521 catctcctcc agttctgagc gactctgccc tgataatatt gaagcggtgc agattgtcaa
    77581 tatcaatatt acaaacagat gcttcaaaat gactcctttt tgtatgatgg cagcctgaac
    77641 tcaatgtcag aatcgtaccc ataaattaaa tcgtcaattg atagtttcac gtgaaacatt
    77701 ttttctctct cctccatcat aatttccgat ggatatttct tacctccaga aacactgaat
    77761 ttgccgaatt gcatgtaaat ctcattatca gagacgtttg cattaatcct ttgattgcaa
    77821 accttcatgt aatcaaggca aatcgtgatg agcctattga aatcctcatt tcctgatgac
    77881 acaacaatct catttctgtc agcactgatc atctcaacat cgtcctcttc aggtatgtct
    77941 ccaaaaataa tagtcataaa atcagaaggc attccattct tcctaagtac catctccttt
    78001 ttgccaatgt aaatttttcg gttaaacctg tcaattacag ctatgtcatc tccaaccata
    78061 agaatcctta ccaactcaat tcccaaagga cctctgacag agccataaaa atcgcctttg
    78121 ctgttcagtc ttgccagtag cccgaatttt ccctctactt cggtaccctc aagttcaatg
    78181 ctccctttac gaatctcaaa acctcttccg gtaatattat ttgaagttac acctctcatg
    78241 acagcgctgt aactgacatc agcagccaat tcatccttaa cagttgtcct cctcttcttc
    78301 atggcagcac atcctgtcat aaggagtatc aatgaaatta ctaccgcgta tttcactgct
    78361 tctggcattt tattatctcc tcctcaagat atgctttctt gctgtcctcc ttcaatgctt
    78421 ccttccagta aacaactgcc tcctcacatc taccaacagc tttctttata aatcccatat
    78481 gctcaagcaa ttcagggtcc ctatcgctgc ttagttcaaa aactctgacc atctcccgat
    78541 gagcatccct gtattttccc atcttgtaca gaacccatgc ataagtatcg acatatgtag
    78601 cgttcccgct ctcagtttca attaccttca ttatcatttt cagtgccttc ttaaggtctt
    78661 tgtcaccttc ggcaagaaag taagcataat tgttcagtag cagcgggtcg tcaggtgaca
    78721 gcttcagggc ctcctcataa catgcatatg aagcatcaaa atctttcttc ctgtagtgca
    78781 ggtctccttc cagagataaa acctgaactt tcagttcatt attatttccg gcaagaatca
    78841 ttgccttctt caactcattt cctgcaacac tatattcacc cagttccatg gcagccagag
    78901 cataatatat ctttccaatc agactcctgt tattatccct ggaataatct ccagccaatt
    78961 cataaagctt actatattca ttcttccggg catatagtat catcaacctc tccttgaacc
    79021 agaaaccctg cctgatactt tttattatct cctcatatct ctgtattgcc aggtcaatct
    79081 tgtcagtagc ttcataaact gccggacgca gtgatataac ctcctcgtca tcaggatatt
    79141 gttcctcaag caacaacaag ctttcgatca ttgattcagc attttccttc atgtatgcag
    79201 aatcacctgc cattcttcgt gcaacagcaa tctttccttc tctctccacc acttcactcc
    79261 tgaagatatt tctcaggaac cctgatatgc cagtatattc ctttctgttt acaaggttca
    79321 ttagatagag gaattgtgat tcaatattat ccggatcttc ctctataatt gccgcgtata
    79381 tgctgtcact cttctccttc aatccattct catagtaaat atcagcaagc aatgccctgt
    79441 agcgggtctc ttcgggattc agctcaagca gcttaatcgc tctctctgcc gcttcatcaa
    79501 ccttattcat gctaataaga tcatatatca tcatcagtga catatccgga gtaagaactc
    79561 cgtcctcttc cagaatctta aagagcgaat cagcctttgc cgtctctcct ctttccgaat
    79621 aaattcctgc cagtattcct ttaacctcca cagcagtggt gtcagccttt attgccctta
    79681 agaaataaac cagagcagag tccttcattt catactgtgt gaacagagag ccgcatgcaa
    79741 gctggtacca gtaatttcct ggttcaagcc ttgctgcagt tgaagcatat tcaagtgact
    79801 tattcctcat cccggctgct gagtacatct gagcaagctc atagtagggc acactccgat
    79861 atttgtccaa ctcaatacac ttctcgaaca gtgaggctgc ttcactcaac tcacctacat
    79921 atttctgtct taatgcctca gaaagtatat agtcataacg attgtcgctc cttgtttccc
    79981 tgtctccttt catgacgggc gaaatcactt tctccgaaca tcctgctgca gccagcaaaa
    80041 tcaccaccag tatgattgta cttctcattt ttctcctgaa tgtccgaatc ctccttcgcc
    80101 tctcacagtc atttcaatac tttcagcttt cttcaatttc acttttacat actgtgatat
    80161 tatcatctgt gcaatcctgt ctccgtcatt tattgtataa tcaatctccg atgtattgat
    80221 caatattacc cctatctctc ccctgtaatc agcatctatt gtgccaggtg agtttagtac
    80281 agtgattccg tgccttatag ccagaccgct tcttggcctg acctgtgctt caaatccaac
    80341 tggcagttca atgtaaaggc ctgtcggaat gagcttcctc tccatcggcc tgatggttac
    80401 gggtccgccg gtaaacgccc tcaggtccat ccctgctgaa tgctctgtct cgtaagctgg
    80461 aagagggtgt ggagacttgt taactatcct tatcttcatg atcctttctt aaacaatatc
    80521 gtaaagtact tatccctgta ttcagcaacg gcaaagaaaa ccaaaagcag catagtgtca
    80581 ataactgcat tgactatttt attgtcgcct gacagcatca tggagataaa caacatcgca
    80641 agcgccagag ctatgtacat aactattcta cccttctcat attctatcgg ataatatttt
    80701 tgcccgacaa agtatgatac aataaccatt gaaagatagc aagctacatg tgcccatgcc
    80761 gcagcaatat atccgtacag aggtacaaaa accaggttca ctgcaactgt cactgcaacg
    80821 cccacaaggg taatccacac gccataaatt gtcttgtcgt caaccttata ccagatactc
    80881 tgattgatgt atatgcccag cagaagatat cccatgctta taatcggcac aacattcatt
    80941 gattcccgga aattttcacc tattatgata cttacaatct ccgggtagag gtttattcca
    81001 agaaacagta tcactgccgt tatcacgaag tacttcattg ttatggcata ggtctccttt
    81061 gcatccttgc ttcctgcctt gtcgaagaag aatggttccg ctgcaaagcg atacatctgg
    81121 atgaaaagcg acataagcac acctaccttg tatcctgctc catacagtcc tacagtggca
    81181 agcccctcag cgcctcctat cagcctgcga agaagaattt tgtcaagtac ttcatttagt
    81241 gatcctgcca tacctcctac aagcaaaggc cagctgtatg ccagcatctt tcttagcagc
    81301 ctcctgtcaa acaccggctt catcctgaaa ataaacggag tcagacataa cagagtaatt
    81361 gctgaaccag ccaggtttgc tacaaagaca tatcccacct gaaaatcagg actccatact
    81421 ttgtaaatcc atctatttcc cttctccgca acagaaggag ctatcttcag gaagaagaat
    81481 gccaatacta tagttacaat cacatttatg atcttcagtg cactgaagag agccgctctg
    81541 ttattgttcc tgagccatgc aaaaggtatt gccgagaatg catctatggc aactatccac
    81601 gcaaacatgc gtatatactc cgggttttct gcataaccaa gaaatccgga aatcggcgta
    81661 ataaacattg agacagccat gatgaacatc gccgaggttg taaagaggct aatcatcgcg
    81721 gtgccgtaca ccacctctgc atttgccttc tttccggcaa acctgaaata ggttgtctcc
    81781 atcccgtagg tcaggatgac cagcgccaga accatccatg catagagctc tgtaacaacg
    81841 ccgtactgct caagaccaag aatacgcgta taaaacggag tcagcagggc ataattcagg
    81901 aatcttggca caaccgttcc aaatccgtat attacagtct gccctgcaag tttgcttata
    81961 tcgttcatgc ttaactctta ctgcaaatat atatctttgt catacgtaaa ctaaaacaaa
    82021 atgaaggctc cattccgtat catcatgaca cttgcagcag ccatcataat tgccggctgc
    82081 tctgcaggta acgagaaagg cgtacgcgcc gtgctacaga ccaccgaagg agaaattgta
    82141 atatctcttt ccgatctcac tccgcttcac cgcgacaact tcgtcaaact tgctgaaagc
    82201 ggtttttaca acggcgtcac ttttcaccgt gtaataaaaa gtttcatgat tcagaccggt
    82261 gatggttcca cacgccaggt ctcatctccc gcaattgaag attatactat tccggcagag
    82321 ataaatgatt ccctcttcca caggagagga gcggttgctg ccgcacgaat gggcgatgat
    82381 gtcaatcctg aacgaaactc ctccggaaca cagttctaca tcgtacaggg aaaggtttac
    82441 aatgatgaag agctggaaaa ggctatagaa cgtatagagt cctcccgcaa tcagtatttc
    82501 tacaacaaag ctcttaagga acttcgcagt gaagcagaac tggccggaaa ggaaatcaac
    82561 gaggatgaga tacagcagtc agcaagcctt aaggcacagg aggccatgga agaggctggc
    82621 gcctatgtta tgcctgaata tcgcagggat gcatacaaga ctatcggagg cgctgttcat
    82681 cttgacggtt cctatacggt cttcggagag gtgctggagg ggatgagcgt tgttgatgcc
    82741 attgcgggta ctgctactga tcagagagac agaccagtga atgacataag aatcatcaag
    82801 gtaaaaattc ttaaataatt gacaattcag agatgaagga gaggatatca gaactaaaca
    82861 aagaggtcga agaattcaat attcacaacg aggaatctct ggagcagttc aggataacct
    82921 accttagcaa gaagggcctg atatcaatgc tttttgaagg attcaggaac gttccgcctg
    82981 aggcgaagcg tgataccgga ctgcttctca ataatctcaa acagtcagca acaaataaat
    83041 acaatgagca taaggagcgc ctggcaatga ttgaggttgc aggtccatct gttgatctga
    83101 ccatgcctgc ctatcctttc gctgaaggca cgcgtcatcc catatccatt gtcaggaatg
    83161 agattatcgg catcttcagc cgtatcggtt tcacagtgtc tgtaggaccc gagatagaag
    83221 atgataatca tgtcttcacc atgctcaact ttgcaagtga tcatccggca cgtgacatgc
    83281 aggatacctt ctttatagaa aagaaatccg gcattgagtc cgatcctcat gatgtgcttc
    83341 tgcggacaca cacatcctcg gtgcaggtca gggtgatgga aaaccagcag cctcccatcc
    83401 gtaccatttc tcccggaagg gtctttcgca acgaagcagt ctcagcacgg gcgcactgta
    83461 tcttccatca gattgaagga ttgtatgttg ctgaaaatgt gtcatttgtc gatcttaaac
    83521 agacgctgct ctattttgcc accgagcttt tcggtaaaga tgtaaacatc aggctcaggc
    83581 cctcattttt cccttttacc gagccctcgg ccgagatgga tataagttgc aacatctgcg
    83641 gcggcaaagg atgcaacgta tgcaagggta caggatggct cgagttgctt ggatgtggta
    83701 tggttgatcc aaatgttctt gatgcctgcg gcatcgattc tgaaaaatat acgggctatg
    83761 ccttcgggat gggaatagag agggcaacca tgctgaaata cggggttaat gaccttaggc
    83821 tttatttcga aaatgatata aggtttctga agcatttccg aactgaaatt ttatgatatt
    83881 aaggaattta tgcccaactt tgtcaaacta tggcaagatt tatgaaacag catgactata
    83941 ccatatcatt ctgtgacaag tgcgcgtttg aaagcagttc ggtttttcgt tttctcacaa
    84001 gagatgagac agacaggttg aatttcgaga aggatttcag acactacaag agaggagaga
    84061 tcctttatca tgagggtaac cggatcagcg gattctattg tatcaactcc gggatcataa
    84121 aggttttcaa gacaggtata gacggtaagg aacagataat cagatttgcc aggaaaggag
    84181 aaataatcgc atacaggtct gtgctgagca atgaacctgc atgtacctcc gcaaaagtta
    84241 ttgaagattg ccaggtatgc tttatccctt cagagcttct catacagttt gtgaagacaa
    84301 atccctcttt tgcctttgag gttgtgaaac tgacatgtca tgagctagcc gaagccaact
    84361 catatataac tgatattgcc cagaagactg taagggaaag ggttgccgag gtattgctca
    84421 gccttgtgag ggattttggt cttgacgagc agaagtacct ccgtatcctg ttgacgcgtg
    84481 aagaacttgc aaacatcgtg ggaacagcca cggaatctgt tatacgactt ctgtcagagt
    84541 tcaaaagcga tcgtcttatt gagcttaacg gacgcaagat caagataatt gaccagaaag
    84601 gtcttgaaaa gataagttcc tgagagagct ctgattgctg ccgctggcgg ctgctggcgc
    84661 agggcggttc ctcggctgat gctggtgcag gatgatgccg tcagtcgtgt ggttccatga
    84721 ctggtgttgc cgcacggtga tgccgtcgca gggtgattac attgcagctg ttgtctcagg
    84781 gtaatgctgt cagccgggtg gttcggtggc ggcggctgtc accttatcag aagagcattt
    84841 cttcccttcc tctcactatc ccgaggtgcc tgtatgcatg ttctgtagcc tcgcggccgc
    84901 ggggagtcct ttttatataa ccctccttta taagaaacgg ttcgtaaacc tcttcaatgg
    84961 tgccgctctc ttctcccact gccgtcgcca cattattcag tcccacgggc cctcccctga
    85021 acttatcgat aatggtcctc agaatgcggt tatccatctc gtcaaggccg tgcttgtcaa
    85081 tattaagcgc ttcaagcgag taccttgtta tgtcaagatc aatagcgccg ctgcccttta
    85141 cctgggcaaa atccctcacc cttctcagca gggcgttggc tattcttgga gtacccctgc
    85201 tgcgcgatgc tatttcagca gcggcatctt catctattgt tatattcatt atcgatgctg
    85261 atcttttgac tattcccttg agaacagcag catcgtagta ttctatatgg aacttaatgc
    85321 cgaagcgggc ccggagggga cttgtaagca atcctgagcg ggttgtggcg cctatcaggg
    85381 tgaacggatt gagcttcaat tgtaccgaac gtgcacccgg tcccttgtca atcattatgt
    85441 ctatctggta gtcctccatt gcggaataga gatattcctc aaccaccgga tgcaaccggt
    85501 gtatctcgtc aataaaaagc acatcaccct cgtcaaggct ggtaagcaac cccgccaggt
    85561 cgcccggctt gtcaaggacc ggacccgacg tgatctttat tttgaccctg agctcgtttg
    85621 aaattattga agcaagggtg gtttttccca gtccgggagg accatgcaga agcacatgat
    85681 caagagcttc gccccgctgc aacgcagctg tgacaaaaat attcaggtta tcggttatgc
    85741 gcggttgccc cctgaaatca gagaaagtca aaggacgaag ttgcctttca agctctgcat
    85801 caccggcctg tgttatctct tttctcaggt tgaactgttc ctccattttc ccttcacttt
    85861 attcgcttta tgctgctctg caggcctgtg ggcacaccgt tgatgctggt ttcaatatcg
    85921 tcacgataag tgatggtaag gattatttcg cccagttcat tatatttctt ccatgtcttt
    85981 gtgcggaagc cattcctgta gtacctgtcc tctttcgtcc tgccgttctc aaagtacagc
    86041 gtatgatggc cctcggggtt tccctgcctg tagtttccct tgaacctgag ctttccgcca
    86101 ggataatatg atttccattc accattgcgg aggccaagga tgtattcacc ctcctcggta
    86161 ttgtcgccgg catttacctt ccatagtccg ttcctttcgc cgtcagcata tgagccttct
    86221 gcaattatct cacccgtgcg ggtgtactcc gtatagctgc cgtccctttt tccctggtaa
    86281 taatcttcct cacgaagcag ttctccttca gggtaatacc accgccatgt gccgtcaata
    86341 cgcccgctgc tgtatgctcc ggcctgctca agccttccgg aactgttgta aaacttccat
    86401 ggtcctgttc tcctgttggt tgtataattg ccctcggcga taagagttcc tccgggagaa
    86461 aaatccttcc atggtcctgt gcggttgcct tcggcatcaa ttatgccctc cgatatcagc
    86521 accccgttgt cattgtatat tcgtgcaccc tttatggttc cgtcactatt atactcgcgg
    86581 tgtatcccta caggaatacc ctctttgaag gggcctgaat agatcagtct tccggaatca
    86641 tcatacctgt tctgtatatc tatctcagcg ctgttgtcaa tatctgttcc ggtcactttg
    86701 ccactttcat acaacagtgt gaccagcaga cgtccccttt catcatactc cttgtaatat
    86761 ccatgaagca catcgtcacg gtagttgcgc tcaatatgcc ggcttccgtc aggataaaaa
    86821 tcgagccact cgccctgttt catacccgac gcatcagtgt agtttattcg ttcgcgactg
    86881 atgagaaaat cattgttgta ttccagaaga gcaatgattt tgccatcatg gttatattcg
    86941 cgcgaaagcc cgtccttttt gctatcagaa tagggtatgg tctgttttat tttcccgtca
    87001 ggaaaataaa tttttgccag gccttccttc ctgtcacctg cataaagctc tgaagagaag
    87061 agataatttc cataaaccgg gtctttcttg tacttcagag aatagccatt ccttttacca
    87121 agcaggtagc taatcttttc aatagtgtca cctgcctggt cataaaaaac ccacaggctg
    87181 tcgaggagaa aactggttcg cctgccttcc gacttgagca ctccggtgac ataataactt
    87241 ttccagtatc cgtctggttt tccgtttctt atatatcctt cactggatat gtttccattc
    87301 gggtacctga actgctgata tccatcctgc agttgcacgt tttgctgcgc agccagcaac
    87361 gcaggaataa aaaccaacat aatcagatat atcttcttca ttttacagcc ccagtttagc
    87421 ggaaatctca agcattcttt ttattggcac tgctgcccat tcgattattg aagcatcaac
    87481 tgtcacttcc ggctcttcaa atttaaggga tttatatatt ttttcaagtg tgatgagctt
    87541 catgaaatta cattcattgt atccgcacat tgaatatttt ggtggtacag gaatgaatat
    87601 tttttccggt tccgcttttc tcatctcata aagtattccc ggttctgtta caacgatgaa
    87661 tacttttccc ggatccttac tgacaaatga gagcattcct gaagttgagc cgatgtaatc
    87721 agctacaagt ctgaccggct tttcacattc cggatgagcg ataatttttg accccggatt
    87781 ttcctttttc agtctaagta tagcatcaag cgagaactgc tcatgcacat ggcatgctcc
    87841 atcccatacg agaatgtcac gctttgtaag ctcttttatg taattgccca ggtttctgtc
    87901 aggtccgaat attatttttt catcctggtg gagcgattca acaatctgtt ttgcgtttga
    87961 tgatgtgcat gcaatgtcac taagagcctt tatgtcagca gttgtattga cataggtgat
    88021 taccgttctt cccggattat catttatgaa ttcttcaaaa tcttcctttt tacatgagtc
    88081 tgcaagggag catccggcca taaga
//
LOCUS       DAOPBK010000002        30766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_1626_length_30766_cov_5.159487, whole genome shotgun sequence.
ACCESSION   DAOPBK010000002 DAOPBK010000000
VERSION     DAOPBK010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30766
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1626_length_30766_cov_5.159487"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..2203
                     /locus_tag="PLA17_00425"
     CDS             47..2203
                     /locus_tag="PLA17_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPK83905.1"
                     /translation="METLVHIKTIAQKFRLHDWQVENTLRLFDDGATIPFISRYRKEM
                     TGSLNEVQLTEIRDEYERLKELEKRREAVIKSIEEQEKMTPELLAKINAALTMSQLED
                     LYLPFRPKRKTRASVAREKGLEPLALWLLEQNQGDPEAEAEKYLGDNLEDTAAALAGA
                     RDIIAEMASENEQVRGSLRRLYERDAVITSKVVKGKEQEGAKFSDYFEWSEPLRRCPS
                     HRLLAMRRGEEEGFLRLTIMPSEEEAQSLVEKQFVKANNDSARQVREAVADGWSRLLA
                     PSMETEFRKTSKEKADEEAIRVFAENLRQLLLAPPLGEKNVLAIDPGQRTGCKLVCLD
                     RQGALLHHDVIYPHPPQNETARSVSRMLSLIDEFAIEAVAIGNGTASRETEQFVRSYL
                     PPESGIKVFMVSEAGASIYSASKTARDEFPDHDVTVRGAVSIGRRLMDPLAELVKIDP
                     KSIGVGQYQHDVDQTRLQASLDEVVMSCVNAVGVEVNTASPHLLTYVSGIGPKMAQNI
                     VDHRTENGPFRSRAELMKVKRMGEKAFEQAAGFLRIRGAANPLDASAVHPESYHIVER
                     MAADLGCSVSDLIKDEIKRKTIRIEKYVTADAGVPTLKDIMQELARPGRDPRQAIQEF
                     SFADIHSIDDVREGMVVPGIVTNITRFGAFVDIGVKQDGLVHVSQMADRYVDDPAKIV
                     KLHQHVTVRVVSVDRTRNRIQLSMKGVGGGKNSRMR"
     gene            2349..4487
                     /locus_tag="PLA17_00430"
     CDS             2349..4487
                     /locus_tag="PLA17_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPK83906.1"
                     /translation="MKRTLLILTAAIIAMTSCGRKPQPPVAEKIPFVVESNGNERVDN
                     YFWMRLSDEQKNAETPDSQTVKVLDWLNAENDYADAVMKHTEPLQQKLFEELKGRIKQ
                     DDETVPYLDNGYWYNTKYLPGREYPVLYRKKDGTDNPEVMLDVNLLAEGHEFTSVSGA
                     SPSPDNNLLAYGIDHVSRRQYTLQFRDMNSGELLPDNILNTTGQTVWAADSKTVFYIS
                     KDPETLRAATVMMHVLGTPSSEDKVVYNEDDETFSVYLGKTKSKKYITIVSDQTLTTE
                     VRLIEAENPAGTVKVFEPRRVDHLYSVEHLNGTFYIQTNADGAKNFKLMSCPENRTGM
                     ASWKEVIPHRPDVLLQNFEVFDNYIVANERIKGLTNLRIISLADKSEHFLDFGEETYT
                     AGISINPNANTTLLRYSYSSLTTPNSVYDYDMAARTKTLLKQDSVLGGFDKNNYETKR
                     LWATAADGTQIPVSLVCRKGFTQDGTAPMLLYAYGSYGSSTNPSFRSNIISLLDRGFV
                     YAIAHIRGGSEMGRQWYEDGKLLKKINTFTDFNDCARFLINEKYTSAEHLYAMGGSAG
                     GLLMGAVANMEPELYNGIIAQVPFVDVVSTMIDETIPLTTFEWDEWGDPRKQEYYDYM
                     LSYSPYDQVKAQDYPNMLVTTGYWDSQVQYWEPAKWVAKLREMKTDDNILILDINMTA
                     GHGGASGRFERLKTTALEYAFMLDLEGIRK"
     gene            complement(4786..6357)
                     /locus_tag="PLA17_00435"
     CDS             complement(4786..6357)
                     /locus_tag="PLA17_00435"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPK83907.1"
                     /translation="MMRIRIEKIRRMLVALILLAVVMPASGQEAAIDLEMLYKIKQEG
                     QRNSDIEELAYIMTDLAGPRLTGSAGMDRGNEIARAKLAEYGLSNVRIEEVSDFNRGG
                     WNLTKAYAAMVAPYYNNFAVTPVAWTAGTNGLVRGEVMLVDIKTPDDIEKYRGKLNGK
                     IIMTPSTTQYEVSFEPLASRYTDEELENLAKADAGGRMGRRFIPGDFLAMRELRNKVN
                     EFLRSENILFSVSQGNAFNIPRSSGSSYDGKGTPPVTEISIPMEAYGRMERMLKGGEK
                     VEMEAEIKAEFTANRKVYNVIGEIPGTDPKLKDQVVLLGAHIDSWHGGTGAADNASGC
                     IVMMEALRILKALDVKPRRTIRVALWGGEEQGLIGSRGYVSKYLYDRENNEKKPGFDK
                     FSVYFNMDNGTGRYRGVYLQENILARSFFEKCMEPVKDMGFTTITSRNTGGTDHQSFD
                     GAGLPGFQFIQDEIEYGRGYHTLADTRERLLVPDLRHNAIITAWLAWNASQQDQLLPR
                     KPQMENQQRMPFFGF"
     gene            6544..9021
                     /locus_tag="PLA17_00440"
     CDS             6544..9021
                     /locus_tag="PLA17_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPK83908.1"
                     /translation="MIYPDTYELKIGFDRIREQLNGCCISPAGIRRVEKMHPSFAAAE
                     VRDRLALTAEFQQILAFGEQFPSDNYNDPYATLEKLRVEGTFPEIEEVVALRGSLDTI
                     RRIIGFFRNRKDGRYPLLTEMTQRVVYYPFVAEAIDRIIDKEGRIRDGASPALKTIRA
                     ELASKNLAAVRKLHAVLRQAQADGIVDRDVTVAVRNGRGVIPVSASNKRGIRGFVHDE
                     SATGKTVFIEPSEVVELNNEITELEHEEKREIVRILTALADTIRPYIDELEENCSFLA
                     EIDFIRAKAIFGNRLNCIMPSVADAPHIRWINVRHPLLTLSFARTAGREVVPLTITIN
                     SRDRILLISGPNAGGKSVCLKTVALVQYMLQCGMTIPAGEGTEAGLFGRFFIDIGDEQ
                     SIENDLSTYSSHLINMKQMLRHADDSSLVMIDEFGTGTEPVLGGALAEAILAELNRRG
                     AYGVITTHYTNLKHFATSQEGISNGAMAFDNQMMQPLFRLDQGKPGSSFAFEIAKKIG
                     LPQEILADAATRAGEGNVDFDRNLREIARDKRYWEKKREAVRRHEKRLEELIATYETQ
                     LGDIKTRKKEIITQAKSKAEEMLRDTNRMIENTIRTIREAQAEKEKTKEVREELDSFR
                     EHLDREEKQVEIPVPPRPRIDTPAASIVKRISARKETPQPVRKKKEALAPGDYVRISG
                     TDTVVQVTGIRGKKVQVVSGSLKMNVDVSMTEPVTEEERRKAASAGSRQHKIDWSLTT
                     RRNQFNPEIDVRGMRTEEALQVVRDFIDTAVMIQFRNLRILHGKGNGILRQMIRQYLE
                     TTGVVKQATDEHVDRGGAGITLVELDV"
     gene            9310..11238
                     /locus_tag="PLA17_00445"
     CDS             9310..11238
                     /locus_tag="PLA17_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HPK83909.1"
                     /translation="MKRLTLLFSALVMLMTLPATGQTNDFYDDAPTMQLKGAKKILIT
                     GEIEKDMTIDLSRLKKHTITVKETTITGGNVGFTGAYRYDGYSLYDILDRVVLKKKNE
                     AEFNPIIDLYVEVTNDRGETAVFSWGEIYYPVNMHRLMIATSVARIVPSKAKDLWPLP
                     TETKVVAGNDLVTSRNISNPVKITVKSLDCNYPVNRGMSPMFSETMKLCVNGKQRAIL
                     TELPEGMDSETYHTVFYGRGMGIHGTTPFIGSMLKELISDYYRLSAEALQKGMVVIAG
                     LDGYRAAFTLSEIVNRNDQEEVLIIDRDNYEGAGRFSIFPACDFFSDRAIKAVMEINL
                     LLPTAGSPWAIVVHGGAGTITRDRMSAGAEAEYRRALQAVIDSGAAVLSAGGSALDAV
                     ERAIRMMEDSPLFNAGKGAVFTHEGRNELDASIMDGSNLAAGAVAGVTDIRNPITAAR
                     TVMEKSVHVMLTGKGASEFAAEQGLEIVDPSYFREESRFRELQRALEREKHGTVGCVA
                     LDMNGDLAAGTSTGGMTNKRYNRIGDAPVIGAGNYANNATCAVSATGHGEFFIRYTVA
                     HDISALMEYSGLSLDEAAREVIKNKLVKAGGTGGIISVDGKGNISMPFNTEGMYRGYK
                     NSDGKNGVFIFSDEPDKL"
     gene            complement(11362..12444)
                     /locus_tag="PLA17_00450"
     CDS             complement(11362..12444)
                     /locus_tag="PLA17_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83910.1"
                     /translation="MKKLFTNQRVTLFAALLCLVALSASAAEVKKEFHREFTPIDNST
                     LTVINKYGSVVTETWDKNFVVVDVTVKVIHSSADRARRILGMISVEFTEEDGNLKAET
                     IFSNEFSSISWRGSNNSFSINYVIKMPARVNFDVTNKYGNTVIDELSGLVRANVRYGN
                     LTVDKLTRGNVKPLNTLMVAYGKATVYELGWAEINARYVGMFSVERATALLVDSKYSK
                     VSVERVSSLVADSKYDGYSIGEANNIVILGGYTDLRFDRVSRKLDVETKYGNLTVNYV
                     PEGFENITVRAGFCAVRLGIDPSACYRLDANSSYGSIKLDDSLFSADRRIIGNTSTSM
                     AGKVGRCSKPSSEVTINTSYGSVRLN"
     gene            complement(12425..12991)
                     /locus_tag="PLA17_00455"
     CDS             complement(12425..12991)
                     /locus_tag="PLA17_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83911.1"
                     /translation="MMNKIEDIIRKNISEFDDSEPSDGHFERFSFKLATRLHAGKARR
                     SIVPYLLRAAVVTLLVTLSSLWTFDHFIRPNLRTKMTLSQVSPEYREVEKYYVQQVKL
                     MEDELTLIDMSNPEQKEALMKEMESMDSVYVELQKELRVNKDDQRIIDAMINHYQTKI
                     EVMSYIIDQLKEIKAETVKPVSHEKVVY"
     gene            complement(12988..13560)
                     /locus_tag="PLA17_00460"
     CDS             complement(12988..13560)
                     /locus_tag="PLA17_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK83912.1"
                     /translation="MEKVEAAFKNIHQDLIDGCRSGDQRAQFQVYKLYYKAMYNTSLR
                     IVNDEMEAEDVMQEAFLSAFEKINTYSGTVSFGAWLKRIVVNRSLDVLGKRKAIFEDI
                     ENHAGIRDDSGEEAAYQEDLALRVEEVKAAIKRLPEGYRVILSLYLIEGYDHDEIAEM
                     FNITSSTSRSQLSRAKQKLVSELKNRKIYE"
     gene            complement(13694..14500)
                     /locus_tag="PLA17_00465"
     CDS             complement(13694..14500)
                     /locus_tag="PLA17_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83913.1"
                     /translation="MKNSLFLIIAIIITVAAAMYQRMTGPTHPYRASASVDGTEYSFA
                     LKRSQTNTHPCLIKLDVPEEVSGTLYFRRFRTNDEWTPVVMKHESGKLAGELPSQPAA
                     GKLEYYIVLKGRSGSETALLREKPVVIRFKGNVPAVIMIPHILVMFFAMLLSNLAGIT
                     ALAGRDSQRKHGRLALWLLIAGGLVLGPVIQKYAFGKFWTGIPFGWDMTDNKTLIAII
                     VWVVAVIMNRRKARPAYTIAAALILLLIYSIPHSLFGSELDYVTGTVTQG"
     gene            complement(14720..15931)
                     /gene="rocD"
                     /locus_tag="PLA17_00470"
     CDS             complement(14720..15931)
                     /gene="rocD"
                     /locus_tag="PLA17_00470"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPK83914.1"
                     /translation="MKPQDYIEREEKYGAHNYHPLPVVLDRGEGPFVWDVEGKRYYDF
                     LSAYSAVNQGHCHPKIIDALTRQAAKLTLTSRAFYNSVLGEYEEYITKYFGYDKVLPM
                     NTGAEGDETALKLCRKWAYLVKKVKENQAKIIVCEGNFHGRTITIVSMSTDPDARKDY
                     GPFTPGFITIPYNDIPALEKALSDPDVAGFLVEPIQGEAGVYVPDEGYIKKAYDLCKK
                     HNVLFIADEVQTGIARTGKLLACDHEGVRPDILILGKALSGGVLPVSAVLADDEIMMT
                     IKPGEHGSTFGGNPLACKVAIAALDVIRDEKLAEKAERLGKIFRDEMRAVKSDMIELV
                     RGKGLLNAIVIRPKNGKEAWDVCLAMKELGVLAKPTHQHIIRFAPPLIITEEQLRDAI
                     SLIKKAILQFD"
     gene            complement(16029..16715)
                     /gene="trmD"
                     /locus_tag="PLA17_00475"
     CDS             complement(16029..16715)
                     /gene="trmD"
                     /locus_tag="PLA17_00475"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPK83915.1"
                     /translation="MRIDIITVLPGLLRSPFDESIIRRARQKGIAEINIIDLRDYSTD
                     KHRSVDDYPFGGGAGMVMMIEPVYNCISHLKSERHYDEIIYTSPDGARFTQAMANELS
                     MKENIIILAGHYKGIDQRIRDHLITREISIGDYVLTGGELPAAVITDAVVRLLPGAIS
                     DAESALSDSFQDGLLAPPQFTRPAEFMGWKVPDILTGGNTPEIEKWRFEQSLRITREK
                     RPDLLDKEES"
     gene            16800..17135
                     /locus_tag="PLA17_00480"
     CDS             16800..17135
                     /locus_tag="PLA17_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HPK83916.1"
                     /translation="MGKFVISKRKNGEYQFNLLAGNGQVILTSEGYASKPSCMNGVKS
                     VMKNATDIKRFESKVAKNGKFHFNLKAANGQVIGTSEMYESESSRENGIASVKRNAPG
                     ATIVDQTKA"
     gene            complement(17136..18824)
                     /locus_tag="PLA17_00485"
     CDS             complement(17136..18824)
                     /locus_tag="PLA17_00485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPK83917.1"
                     /translation="MRQTILMIIALLLTVGLGCTKKEKADLVITGGVVFTADSLNPRA
                     EAVAVKGNRILAAGSDREIGRYIDPQVTRVIDAGGRAVLPGFNDAHAHFGPVDPDYIE
                     LRYVTDPSVITNKVKEQVARSQKGQLIYGGHWEHEMFATREWPTKELIDAVSPDNPVV
                     LRRTDGHSALVNSYVLRQSGITRNTPDPFGGEIMHDPVTGEPTGILKESAMSLIRTGA
                     IAVERTPEEERARAWNGYLMALQMAAGYGITSIQIPGSANFDMYDSLMAAGKLSCRID
                     IGGTLTADPERLDRYETLREKYLASGDWIRFGYLKDFMDGTLGSATAMMFEPFADAPD
                     KSGLPQMSREELEARVLAADSRGFQIGIHAIGPKANNWILDAFEKAREVNGIRDSRHR
                     SEHAQILIEEDIPRFAELGVIASMQPVHCITDKRFAEKRIGIERSRWAYAWKTLAESG
                     AKLAFGTDYSVEPLNPMEGLYASVTRKDRKGEEGDGWFPMEKITMEKAIEYYTLGAAY
                     AQFMEDRKGMLRKGYLADITILSENIFNTPEEMIMSVKSDYTIVDGRIVYERKE"
     gene            18920..20221
                     /locus_tag="PLA17_00490"
     CDS             18920..20221
                     /locus_tag="PLA17_00490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK83918.1"
                     /translation="MKNKIIPPGYLYSKRYTNYVFILLFLLYMFDYIDRMIVTSMFVH
                     IEQDLGITHTQSGLLVSAVYWSIVLLTFPVSILVDRWNRTKTIGIMAALWSIATALCA
                     LTGNLIQLVFARALIGVGEAGYAPGGTAVISGLYPEDKRSKMMGLWNASIPLGTAIGV
                     LLGGIIAARLGWKHAFGIVALPGFIVAVLFFFVKDYKTVDLSFTNSNNVKVKMKKKDM
                     AREFLSRPSLIFTYFGFAAVVFVTTSLITWLSTYFNVTRGLSSEKAGTMASAVMLLAI
                     VGAPLGGIITDRWRKSNIRARLLFPTISSLLTALFLLVALWLTSGTLQYVFFLLMGVT
                     VAAFIPAAAAVTQDVIHAGMRAMSYAVAVVVQNLLGASTAPLVIGRLYDLYDIETALA
                     ILPFMLILGAFLFYMGSRFYVRDLNKTAKIKLEAETNNRMR"
     gene            20218..21618
                     /locus_tag="PLA17_00495"
     CDS             20218..21618
                     /locus_tag="PLA17_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPK83919.1"
                     /translation="MKELLRNKYFKFGVAGVLYILWVIWVGNYWLLLGLPIVFDIYVS
                     KKVNWAFWKKRHGKNHVVVEWLDALIFAVIAVSLINIFLFQNYRIPTPSMEKTLLVGD
                     HLAVSKVAYGPRMPNTPLVFPFTQHTMPFTGGRVKSWSDLIHWKYNRLKGFGHVKNND
                     IVVFNFPAGDTVCLEQQAVSYYEILRERIETITAEDIRAGRPLKGKDEYYSIARQMIW
                     NEYTVIYRPVDRRDNYVKRCVGIPGDTVLIKGGKLFINGHENTEYETQQTSYYVRTNG
                     TRINPKAFERMGVSKSDQSLTISGSGYKLPLTRKNAETISGFANVTGIEADYRRPGEY
                     IAAVFPHDPAYRWNEDNFGPLWIPKKGTTVKLDMTNINFYTSIIDIYEENDLEIRDTV
                     IYINGKPADSYTFRMDYYWMMGDNRHDSADSRFWGFVPEDHIIGRPVRVWLSIDKEAQ
                     GLKKIRFNRFFKGAKR"
     gene            complement(21707..22054)
                     /gene="rplT"
                     /locus_tag="PLA17_00500"
     CDS             complement(21707..22054)
                     /gene="rplT"
                     /locus_tag="PLA17_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPK83920.1"
                     /translation="MPRATNAPASKARRKKILKQTKGYFGKNANVYTVAKNVLEKGLT
                     YAYRDRKNKKGQFRALWIQRINAGVRLHGMSYSQFIGGLEKKGISLNRKTLADLAMNH
                     PEAFKAIVDKVKE"
     gene            complement(22156..22353)
                     /gene="rpmI"
                     /locus_tag="PLA17_00505"
     CDS             complement(22156..22353)
                     /gene="rpmI"
                     /locus_tag="PLA17_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPK83921.1"
                     /translation="MPKVKTNPGAKKRFSLTGSGKIKRKHAYKSHILTKKSTKRKRNL
                     TYSGLVDKADQKNVKLLLGLK"
     gene            complement(22379..22933)
                     /gene="infC"
                     /locus_tag="PLA17_00510"
     CDS             complement(22379..22933)
                     /gene="infC"
                     /locus_tag="PLA17_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPK83922.1"
                     /translation="MAGPTFSRPRPGRKPEPEHRINNRITARTVRVVGENVETKIMSI
                     SDALRLADEMELDLVEISPNADPPVCKIVDYQKLLYQQKKKQKEIKANAARIVVKEIR
                     FGPNTDDHDYNFKLKHAINFLEEGAKVRAYVFFKGRSILFKDQGEKLLARFVSDLDEY
                     GKVDQAPRLEGKRMIIILSPKKKK"
     gene            complement(22981..24921)
                     /gene="thrS"
                     /locus_tag="PLA17_00515"
     CDS             complement(22981..24921)
                     /gene="thrS"
                     /locus_tag="PLA17_00515"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPK83923.1"
                     /translation="MISITFPDGSVREYRQGITSHEIAKSISPGLAADVYAATVNGEV
                     RDLSQPVEGNATVRFHKWDDTEAKHAFWHSSAHLMAEAIEALFPGTKFGIGPAIENGF
                     YYDVDPGDGKVITDSDLPLIEEKMKELAAGKSPIVRMEISKKDAMELFTAKGDEYKTE
                     LISELEDGTITLYRQGNFTDLCRGPHLPDTSSIRAIKVLSLAGAYWRGDEKRKMMTRI
                     YGITFPKQKMLDEYMTMLEEARKRDHRRIGRELELFAFSAKVGMGLPLWLPKGADMKQ
                     RLIDFMTKVLRERGYTIVSTPHIGNIELYKTSGHFEKYGADSFRPITTPQDGEVFMLK
                     PMNCPHHCEIFNATPHSYRELPVRMAEFGTVYRYEQSGELHGLTRVRSFTQDDAHHFC
                     APEQLKEEFKSIIDLVQYIFKIAGFNEFTAQISLRDPDNREMYIGSDENWEKAEKAII
                     EAAGEMNLITTVAVGEAAFYGPKLDFMVRDAIGRKWQLGTIQVDYNLPERFGLEYTGS
                     DNQKHRPVMIHRAIFGSMERFVAVLIENTAGRFPVWLTTDQAVIVPISEKFNDYAKTV
                     LNFLNNYDIRTLIDDRNEKIGKKIRDNELKRIPYLLVIGEKEELAGTVSVRKQGEGDR
                     GVMKPEEFVNLVKEEIKRETGV"
     gene            25071..25760
                     /locus_tag="PLA17_00520"
     CDS             25071..25760
                     /locus_tag="PLA17_00520"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPK83924.1"
                     /translation="MKRVLILTAAAALMMLNSCDIQPESHFFADKIKAEIGEDIFFFN
                     RSYNATDYEWDFGDGTFSNAYEPSHAYNGSGIFTVILTAYSKSGSIDKSYLDIEIISP
                     TMLEIEVLEYYDQYPVSGASVILYPTEKDWDNETNAIVEGFTNASGKVVFTNLQPRVY
                     FVDVWHSTHNNYTLRDEDTGFIRTDQLEKNQLNKFIAWVDYTGTKGATARDRKQLVPL
                     KSRTVSATVKK"
     gene            25854..27584
                     /locus_tag="PLA17_00525"
     CDS             25854..27584
                     /locus_tag="PLA17_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPK83925.1"
                     /translation="MDTKFALHGIDYLIMVVYFVFVLGIGWALRKYMKNASAFLEAGR
                     SIPAWVAGLAFISTNLGALEVMGMTGSGLKYGMLTTHFYWIGAIPAMVFLALFMMRFY
                     YGSKARSVPEYLKLRFNEKTRALNAFLFALMTVLASGISMYALAILLENVLGWNFNLS
                     LLASATIVLGYTYLGGLSSAIYNEVLQFFIIIIGLLPMTILVLVDVGGYGGMQASLAP
                     IVEGKGFAPDTWTTTWKYTANASANPMGVEWYGILAGLGFVLSFGYWCTNFLIVQRAM
                     AADSMTSARKVPLIGAIPKMFIPFLIIIPGIAALVLLNDPGKGFGLPLNEAGQPIYDY
                     TIIMLLKQYLPAGVLGFGITALLASFMSGMAGNVSAFNTVWTYDIYQSYIRPATGDKE
                     SDDRHYLKVGRMATIIGVLVSIAAAYIASKFGNIMDFLQTVFSMINAPLFAVIFLGMF
                     WKRSTGNAAFTGLLAGFLIALLHHGLTMPEGATTLVKGGWIGVVHTYPVEMAQNFWTA
                     IFAFTMAALLTVIISLLSKRDKSDAELNGLVYSMTPKLDDSGVIWYKRPWFLALIVGV
                     IMIVLSIMFW"
     gene            27603..27812
                     /locus_tag="PLA17_00530"
     CDS             27603..27812
                     /locus_tag="PLA17_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83926.1"
                     /translation="MLDIRFPIGLMFSIVGLIITIYGLATMGQDAMYVKSLNVNVNLL
                     SGIGCLIFGLLMLLFSDPVRKRNKK"
     gene            complement(28023..29171)
                     /gene="lysA"
                     /locus_tag="PLA17_00535"
     CDS             complement(28023..29171)
                     /gene="lysA"
                     /locus_tag="PLA17_00535"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPK83927.1"
                     /translation="MITREITEKLGKHQTPFYYYDLDILRETLSVASREAAGRGFHVH
                     YAVKANFNPVIMKIIAKQGFGADCVSGNEVEHAIACGFPVSETVFAGVGKSDTEIEKA
                     LRLGIKCFNAESAEELNVIDSIAGRMGKKAPVALRINPNVQAHTIKPITTGTDENKFG
                     IRLPDLDNVLDRLKELQNIRYRGIHFHIGSQITDLNVYRNLCTRINELWQWLTGRGFG
                     PEDINVGGGLGVSYNDPDRIPPFADYFNIFSTSLDSRIRSTISFELGRSLTANCGSLV
                     SRVLFVKPGALETFVILDAGMTELIRPALYHARHSIKNLTSTGPVKKYTVVGPVCETT
                     DTFGRNIQLPETRRGDLIVIRSAGAYGEVMASRYNMRSLPAALFSDSL"
     gene            complement(29177..30490)
                     /locus_tag="PLA17_00540"
     CDS             complement(29177..30490)
                     /locus_tag="PLA17_00540"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPK83928.1"
                     /translation="MKVYKFGGTSAGSPERLRALIPLINDGGLKIVVLSAMAGTTSSL
                     AEISGLLGSGDKSGAERMTGQLRSKYHAVISDLFASKEWSKRAHDLADEHFDFIASLI
                     SGEPARGTDKMILARGELISTALLHFLLTEQGIPSLLLPALDFMRVDKDGEPDYFYIS
                     ENLKRGLSAVRDEKIIITQGFICRNVFGETDNLRRGGSDYSATIIGAAIGAEEVQIWT
                     DISGLHNNDPRYVENTRVVRRLSFNEAAELAFFGAKILHPLSINPARMKNIPVRLKNT
                     MEPADEGTLITSETNLEDYTAVAAKDGITSIRIHSGRMLMAYGFMRRIFEVFETFRTP
                     IDMITTSEVSLSLTIEDTTHLEEIVKSLSSIGQVKTEHNQTIICVVGDFRPERVGSAP
                     EIFEALNEIPVKMISYGGSDHSVSILINTTDKITALRALNEKLFA"
BASE COUNT         7620 a         7874 c         7599 g         7673 t
ORIGIN      
        1 gagcgaatac ctgaaaggat tgattcttta cattgaataa tgatctatgg agactttggt
       61 acatataaaa actatagcgc aaaagttcag gctgcacgac tggcaggttg agaatactct
      121 gagactcttt gatgatggcg ccacgatacc attcatcagc cgctaccgca aggagatgac
      181 gggaagtctc aatgaggtgc agctgactga gatacgtgat gaatatgaga ggctgaagga
      241 gcttgaaaag cgaagggagg cagttatcaa atcaatagaa gagcaggaaa aaatgacccc
      301 tgaacttctg gcaaagatca atgcagctct gactatgtca cagcttgagg atctttacct
      361 gcccttcaga cctaaaagaa aaacccgagc ctccgttgcc cgcgagaaag gcctcgaacc
      421 cctggcactc tggctgcttg aacagaacca gggtgaccct gaagcagagg ctgaaaagta
      481 tctgggtgat aatttggaag atacggcagc tgccctggcc ggcgcacgtg acataattgc
      541 cgagatggcc agtgagaatg aacaggttcg cggatcgctc aggcgtctct atgagcgtga
      601 tgcagtcata acctcgaagg ttgtaaaggg aaaggaacag gagggagcca agttcagcga
      661 ttatttcgaa tggtcagagc cgctgcgccg ctgcccgtca cacaggctgc ttgccatgcg
      721 ccgcggtgag gaggaggggt tcctcaggct taccattatg ccctctgagg aggaggcgca
      781 gagcctggtt gagaaacagt tcgtgaaagc aaacaacgat tcagcccggc aggtgcgcga
      841 ggcggtggct gacggctgga gcaggcttct ggcgccgtca atggagacgg aattcagaaa
      901 gacatccaag gagaaagccg acgaggaggc catacgcgtt ttcgcggaga acctgcggca
      961 actgctgctg gcgcctccgc tgggtgagaa gaacgtactg gcaattgacc cgggacagag
     1021 aacgggctgc aagctggtat gccttgaccg tcagggagct ctgctgcacc atgacgtgat
     1081 ctatccgcat ccgccacaga acgagacggc acgcagcgta agcaggatgc tttcacttat
     1141 tgatgagttc gccattgagg ctgtagctat cggcaacgga accgccagtc gcgagaccga
     1201 gcagttcgtc cgttcatatc tccctcctga atcgggaatt aaggtcttca tggtgagtga
     1261 ggcgggtgct tcaatatatt cagcttcaaa gactgcccgc gacgagtttc ccgatcatga
     1321 cgtgacggtg cgcggagccg tgtcaatagg ccggcgcctg atggacccgc tggctgaact
     1381 ggttaagata gaccccaaat cgataggggt ggggcagtac cagcatgatg ttgatcagac
     1441 acgcctgcag gccagcctcg acgaggtggt gatgtcatgc gtcaatgccg tgggtgtgga
     1501 ggtgaacacc gccagcccgc accttctgac ttatgtctca ggcataggcc cgaaaatggc
     1561 tcagaacatc gtcgatcacc ggacggagaa cggcccgttc cgctcacgtg ccgagctgat
     1621 gaaggtgaaa cggatgggtg aaaaggcttt tgaacaggct gcgggattcc tcaggatacg
     1681 aggcgctgct aatccgcttg acgcctcggc cgtgcacccg gaaagctacc atatcgtgga
     1741 gaggatggcc gctgatctgg gatgctccgt ctctgatctt ataaaggatg agattaaacg
     1801 aaaaaccatc aggatagaga aatatgttac tgctgacgca ggagtgccaa ccctgaagga
     1861 tatcatgcag gagcttgcca gaccgggacg tgatccgcgc caggcgatac aggaattcag
     1921 ctttgcagat atacactcca ttgatgatgt cagggaggga atggtggtcc cgggtatcgt
     1981 gaccaacatc accaggttcg gtgcatttgt tgatatcggc gtcaagcagg acggtcttgt
     2041 gcatgtttca cagatggctg accgatatgt tgatgatccg gcaaagatcg ttaaattgca
     2101 ccagcatgtc acggtccgtg tcgtttcggt tgaccgtacc cggaaccgga ttcagctcag
     2161 catgaaagga gttggcggcg gtaaaaattc ccgcatgaga taaagcattg gcgtgggggt
     2221 ggctgcagtg cgaaacgcat tatacccgaa tgaaacacag attgagtgaa aacagaagca
     2281 aaggcaaagc tacgtgcgaa acgcattata ccagatagaa acaatgttat aacactaatt
     2341 attaaccaat gaaaagaacc ttattaatcc tcactgccgc gatcatcgcc atgacatcat
     2401 gcggcaggaa accccagcct cctgtggctg aaaaaattcc gttcgtcgtt gaaagcaacg
     2461 gtaacgaacg agtcgacaac tacttctgga tgcgcctgag cgatgaacag aaaaatgcgg
     2521 aaacacctga ttcccagacc gtgaaggtgc ttgactggct caacgctgag aatgattatg
     2581 ctgatgcagt gatgaaacat accgaacccc tgcagcagaa actcttcgaa gagctcaagg
     2641 gccgtatcaa acaggatgat gaaactgtcc cttacctcga caatggctac tggtacaata
     2701 ccaaatacct tccgggcagg gaataccctg tactctacag gaaaaaggac ggcaccgaca
     2761 acccggaggt gatgcttgac gtcaacctgc ttgccgaggg tcatgaattc accagtgtca
     2821 gcggcgcctc accctcgcct gacaataacc tcctggctta cggtattgac catgtcagcc
     2881 gcaggcagta cacgctgcag ttcagggata tgaacagcgg cgaactgctc cctgacaaca
     2941 tcctcaacac caccggccag acagtatggg cagctgacag taaaacagtg ttctatataa
     3001 gtaaggaccc tgaaaccctg agggcagcaa ccgtcatgat gcatgtactc ggtaccccat
     3061 cctcggaaga caaggtggtg tacaatgagg atgacgaaac attcagcgtt tatcttggaa
     3121 aaacaaagag caaaaagtat atcacaatcg tctctgacca gacgctcaca accgaggttc
     3181 gcctcattga ggcggaaaac ccggcaggca ccgtgaaggt attcgaaccc cgcagggttg
     3241 accatctcta ctccgtggaa cacctgaacg gaaccttcta tattcaaacc aacgccgatg
     3301 gcgcgaagaa cttcaaactg atgtcgtgcc ccgaaaacag gacaggtatg gcatcatgga
     3361 aagaggtcat tccccatcgc ccggatgtgc tgcttcaaaa ctttgaggta tttgacaatt
     3421 atattgttgc aaatgagcgc atcaaaggcc ttacaaacct gaggatcata agcctggcag
     3481 acaaatcaga gcatttcctt gatttcggtg aagaaacata tacggcaggc atcagtatca
     3541 atccgaatgc aaacaccacg ctgctccgct acagctattc atccctgacc acacccaact
     3601 cggtctatga ctacgacatg gctgcaagga caaagactct gctgaaacag gatagtgtac
     3661 tgggcggttt cgataaaaac aactatgaga cgaagcgcct atgggccact gctgccgatg
     3721 gcacacagat tccggtctcg ctggtctgcc gaaagggatt cacacaggat ggtacagctc
     3781 cgatgctgct ctatgcatat ggttcatacg gctcttccac caatccctca ttccgctcaa
     3841 acatcataag ccttcttgac aggggatttg tctatgccat cgcccacatc aggggtggca
     3901 gcgagatggg ccgccaatgg tatgaggacg gcaagctgct gaagaagatc aacactttta
     3961 ccgacttcaa tgattgtgcc cgttttctga taaatgagaa gtatacctct gccgaacatc
     4021 tctatgcaat gggcggaagc gcaggcggat tgcttatggg tgctgtcgca aacatggaac
     4081 ccgaactcta caatggcatc attgcacagg ttccgtttgt tgatgtggta agcaccatga
     4141 ttgatgagac tattccgctc acaactttcg aatgggatga atggggcgat ccccgcaagc
     4201 aggagtacta tgattacatg ctttcatatt caccttatga tcaggtgaag gctcaggact
     4261 atcccaacat gcttgtcaca accggttact gggattcaca ggtgcagtac tgggagcccg
     4321 ccaagtgggt tgccaaactc cgcgagatga agactgatga taatatccta atactggata
     4381 ttaacatgac tgccggtcac ggcggcgctt caggcaggtt tgaaaggttg aagacaaccg
     4441 cactggagta tgccttcatg cttgaccttg aaggaatcag gaagtaaatg acccgtctcc
     4501 ggcccggtga tagccgggga cacacgtgcc cgtgcaataa aagaacagag gctggccctt
     4561 ttcgcggtca gcctctgctg taacaaacag aattgaaaaa caaatttctt tatgttttct
     4621 gcagatattt tactgcagaa cctccgggtt tttgcggctt cgtcagaggg acgcagctgc
     4681 ttttgtcccg ggaaactgcc actgagatca cccttgatac tgccccgggg ttcgaccggg
     4741 atacgcccgt ggagatccgg ggacacacgg atactgcccc gggaatcaga acccgaagaa
     4801 gggcatccgt tgttgattct ccatctgggg cttgcggggc agcagctgat cctgctgtga
     4861 tgcgttccac gccagccatg ccgttataat tgcattatgc ctcagatcag gaaccaggag
     4921 acgttcacgg gtgtcagcaa gtgtatgata accacggcca tactcaatct cgtcctgaat
     4981 gaactggaac ccggggagac ctgcgccatc aaagctctga tggtcggtgc caccggtgtt
     5041 cctgcttgtt attgtggtga atcccatgtc tttaaccggc tccatgcact tctcgaagaa
     5101 tgatcttgca agaatgttct cctggaggta gacaccgcgg taacgaccgg taccattatc
     5161 catgttgaaa tatacgctga acttgtcaaa cccgggtttc ttctcgttgt tttccctgtc
     5221 atacagatac tttgagacat atccccttga cccgataaga ccctgttcct caccacccca
     5281 gagagccaca cggatggtcc tgcgcggttt gacatcaagt gccttcagta tgcgaagtgc
     5341 ttccatcatc actatgcagc ccgatgcgtt gtctgcagct cctgtgccgc catgccagga
     5401 gtcgatatgc gcaccaagaa gaacaacctg atccttcagc ttcggatcgg tgccgggaat
     5461 ctcgccaatg acattgtaaa ctttcctgtt ggcagtgaat tcggccttta tctcagcctc
     5521 catctccact ttctcaccgc ccttcagcat tctctccatt ctgccatagg cttccatcgg
     5581 gatgcttatc tcagtcaccg gaggtgtgcc tttgccatca taggatgagc ctgatgatct
     5641 ggggatgtta aaggcatttc cctgcgagac cgaaaacaga atattctcac tcctgagaaa
     5701 ctcgttcacc ttgttgcgca gttcacgcat cgccaaaaag tcacccggaa tgaaccttct
     5761 tcccatacgg cctccggcat cagcctttgc aagattttcc agctcctcat cggtatacct
     5821 ggatgccagt ggctcaaagc tgacctcata ctgtgtagtg gagggtgtca tgatgatctt
     5881 gccattcagc ttgccacggt acttctcgat gtcgtccggg gttttgatgt caacaagcat
     5941 aacctcaccc cttacaaggc cgtttgtccc ggctgtccac gccaccggag taacggcaaa
     6001 attgttgtag tagggagcca ccatagctgc atacgcttta gtcaggttcc atccgccgcg
     6061 attgaaatca ctcacttcct caattcgtac gtttgacaaa ccatattctg ccagctttgc
     6121 ccgtgcaatc tcattacccc tgtccatgcc ggcagatcct gtgagccttg gccctgcaag
     6181 gtcagtcatg atataggcaa gctcttcgat gtcagaatta cgctgaccct cctgtttaat
     6241 cttgtataac atttcaaggt caatggcagc ttcctgtccg gaagccggca taaccactgc
     6301 aagcaggatg agtgcaacaa gcatccttct gattttttca atacggattc tcatcatgaa
     6361 ataattttga ttttattaag gtaagaagga acccacatga ttgaaaaaaa gtgtttgtct
     6421 gtttgataca tgtaggtttc attttatttt gtggttaacg ggattaaaga taaattcaga
     6481 agttttatac ccgtgcagtg ttaacattaa ttaacgttac ttttgtgagg agatgaccat
     6541 cagatgatct atccggacac atacgaactg aagattggct ttgaccgtat cagggaacag
     6601 ctcaacggat gttgcatcag ccctgcagga atccgcaggg ttgaaaagat gcacccctcc
     6661 tttgctgcag cagaggtcag ggacaggctt gcccttacgg ccgagttcca gcagattctt
     6721 gcctttggcg aacagttccc ttctgacaat tacaatgatc catatgcaac cctggaaaag
     6781 cttcgcgtgg agggtacttt cccggagatt gaagaagtgg ttgccctgcg gggatcactt
     6841 gataccatac ggcgaatcat aggctttttc cgtaacagga aggacggacg atacccgctg
     6901 ctgacggaaa tgacacaaag agtggtttat tacccgtttg ttgcagaggc tattgaccgc
     6961 attattgaca aagagggcag gatacgtgac ggcgcctccc cggctcttaa aacgatacgc
     7021 gccgaactgg cttcaaagaa cctcgcggca gtgagaaaac tgcacgcagt attgcgccag
     7081 gcacaggccg acggcattgt tgaccgggac gtgacggtgg cagtgcgcaa cggcaggggt
     7141 gtgatacccg tcagcgcatc aaataaaaga ggaataaggg gattcgttca tgatgagtct
     7201 gcaacaggaa aaacagtatt cattgagcct tcagaggttg tggaactcaa caatgagatc
     7261 accgagctcg aacacgagga gaagcgtgag atagttcgta tcctgacagc cctggctgac
     7321 accatcaggc catacatcga cgaactggag gagaattgca gcttcctggc agagatcgac
     7381 tttatcaggg caaaagccat ctttggcaac aggctcaact gcatcatgcc ttcagtggct
     7441 gatgccccac acattagatg gatcaatgtc cgccatccgc tgctgacact ctcttttgcc
     7501 cgcacggcgg gacgggaggt ggtgccactg actataacca tcaacagcag ggacagaata
     7561 cttcttatct ccggccccaa cgccggagga aaatcagtct gccttaaaac cgtggcactc
     7621 gtccagtaca tgctacagtg cggaatgaca atcccggcag gcgagggcac cgaagctggc
     7681 ctgttcggca gattctttat cgatataggc gacgagcaga gtattgaaaa tgacctcagc
     7741 acctacagct cacacctaat caacatgaaa cagatgctca ggcatgctga tgactcctca
     7801 ctggtcatga tcgatgagtt tggcacaggt accgaaccgg ttctcggcgg agccctcgcg
     7861 gaagcaatcc tggcagaact caacaggagg ggcgcttacg gggtgattac cactcactat
     7921 accaatctca agcacttcgc aacttcacag gagggaatca gcaacggggc aatggctttt
     7981 gacaaccaga tgatgcagcc cctcttccgc cttgaccagg ggaagccggg gagctcattc
     8041 gccttcgaga tagccaaaaa aataggcctt ccgcaggaga tactggcaga tgctgcgaca
     8101 agggcaggag aggggaacgt tgacttcgac aggaatcttc gcgagattgc ccgtgacaag
     8161 cgctactggg aaaagaaacg cgaggcagtg cgccgccacg agaaacggct cgaggaactg
     8221 attgcaacct acgagacaca gctcggcgac ataaagaccc ggaaaaagga gattattacc
     8281 caggctaaaa gcaaggcgga agagatgctt cgcgacacca accggatgat agaaaacact
     8341 atccggacca tccgcgaggc acaggcggag aaggagaaaa cgaaagaggt acgcgaggag
     8401 cttgactcat tccgtgaaca ccttgacagg gaagagaaac aggttgagat acctgttccg
     8461 cccagacccc gcattgacac ccccgcggcc agcattgtga aaagaatctc ggcaaggaag
     8521 gagactccgc agccggtcag aaagaagaag gaagcgcttg cgcccggcga ctatgtcagg
     8581 atctccggca ccgataccgt ggtgcaggtg accgggatac gcggaaagaa agtgcaggtt
     8641 gtctccggca gccttaagat gaatgtagac gtcagcatga ccgaaccggt caccgaagag
     8701 gagcggagaa aggccgcctc ggcaggcagc aggcagcata agatcgactg gtcactcaca
     8761 acccgccgca accagttcaa tcctgagatt gacgtgcggg gcatgcgcac tgaagaggcg
     8821 ctgcaggtgg tccgggattt cattgatacc gccgtgatga tacagttcag gaacctgaga
     8881 atactgcacg ggaagggtaa tggcattctg aggcaaatga tcaggcagta cctggaaaca
     8941 accggggtgg taaagcaggc tacagatgaa catgttgacc ggggcggagc cggaatcacc
     9001 ctggtggagc tggatgtctg atgagcggag ccggaatcac cctggtggag ctggatgtct
     9061 gatgggcggg gaatgtggtt gggtttccgg tgggtcggag gtctctgttt ccgtatggca
     9121 ggccggcact gtggtccggt attctgcctg gcattatagc gacagattgg cactgcggcc
     9181 cggtattctg actggtccgg caacggcagg ccggcaccta aacctgcaat atatcatttt
     9241 gcctgaagca tgaattgaat agatttgcat aacgtattga atgaatttgc ataacataaa
     9301 acctgaatca tgaagagact gacgcttctt ttttccgccc tggtgatgct gatgacgctt
     9361 ccggcaaccg ggcagaccaa tgatttttat gatgatgccc ccacaatgca gctgaagggc
     9421 gccaaaaaga tccttatcac aggtgagatt gaaaaggata tgacaattga cctgtcacgc
     9481 ctgaagaaac ataccatcac cgtaaaggag acaacaatca ccggcggaaa tgtcggcttc
     9541 acaggagcat accgctatga cggctattca ctgtatgata ttctcgacag ggtcgtcctg
     9601 aagaaaaaga acgaagcaga attcaacccg atcattgatc tctatgttga ggtcaccaat
     9661 gacaggggtg aaaccgcggt attctcatgg ggtgaaatat actacccggt gaacatgcac
     9721 aggctgatga ttgccacctc cgttgcccgc atcgtgccct ccaaggcaaa ggacctctgg
     9781 ccgttgccaa cggagacgaa ggttgtggcc ggcaatgacc tggttacatc gcgcaatatc
     9841 agcaacccgg tgaaaatcac cgtgaaatca ctcgactgca actatcccgt caaccggggg
     9901 atgagcccga tgttttcaga gacaatgaag ctatgcgtca acggaaaaca gcgtgccata
     9961 ctgacggagc ttcccgaagg aatggatagt gagacctatc atacagtgtt ctacggacgc
    10021 gggatgggta ttcacggtac cacacccttt atcggctcaa tgctgaaaga gctgatcagt
    10081 gattattaca ggctctctgc cgaagcgctt cagaaaggta tggtagtcat cgccggcctc
    10141 gacggttacc gtgcggcatt cactctgagc gagatagtta accgtaatga tcaggaggag
    10201 gtgctgatca ttgatcgtga taattacgag ggcgccggcc gattctctat cttcccggca
    10261 tgtgattttt tctctgaccg tgccatcaag gcggtgatgg agatcaatct ccttctgcca
    10321 acggccgggt ctccgtgggc tattgttgtg catggcggcg ccggcaccat cacccgtgac
    10381 cggatgagtg ccggggctga ggctgaatac cgcagggcac tgcaggcagt gatcgacagc
    10441 ggcgccgcag tgctcagcgc aggcggctcg gcccttgacg ccgtagagcg tgccatccgg
    10501 atgatggaag attcacctct cttcaacgcc ggcaagggcg ctgtattcac ccacgaggga
    10561 agaaatgaac tcgacgcctc catcatggac ggaagcaacc tcgccgccgg ggccgtagcc
    10621 ggagtcacag atatccgcaa ccccataacc gcagcacgca ccgtaatgga gaaatcagtg
    10681 cacgtaatgc tgacaggcaa aggcgcatct gaatttgccg ctgaacaggg cctcgagata
    10741 gttgacccct cttacttccg cgaggaatca cgcttcagag aactgcaacg cgctcttgag
    10801 agagaaaaac atggcaccgt gggctgcgtg gcccttgaca tgaacggcga cctggcagca
    10861 ggaacctcca ccgggggtat gacaaacaaa agatataacc gcattggaga tgcaccggtg
    10921 ataggagcag gaaactatgc caacaacgcc acatgcgcag tctccgcaac cggccacggt
    10981 gagttcttta tcaggtacac cgttgcccat gatatctctg cactgatgga gtacagcggc
    11041 ctctcacttg atgaagcagc ccgtgaggtg atcaaaaaca aactggtgaa ggccggcgga
    11101 acaggaggaa tcatatctgt cgacggcaag ggcaacatca gcatgccatt caataccgaa
    11161 ggaatgtacc gcgggtataa gaattcagat ggcaaaaacg gcgtcttcat cttctccgac
    11221 gaacctgaca aactttaatg acaaaaaaac gcggcgccct ctctctggcg ccgcgcttct
    11281 ttttaccgtc cctgcccggt taagcctgac tgaaacgcag tagtcataac gccaggctta
    11341 ctccacgctt cccggtcttg cttagttaag cctgactgaa ccataggatg tattgatggt
    11401 aacctctgat gatggcttgc tgcatctgcc aaccttgcct gccatactgg ttgatgtgtt
    11461 gccaatgatc cgcctgtcgg ccgagaaaag agagtcatca agcttgatgc ttccgtagga
    11521 gctgttggca tcaagcctgt agcaggccga aggatcaata ccgagcctga cggcacagaa
    11581 gcctgccctg accgtaatat tctcaaatcc ttcaggcaca tagttaactg taagattccc
    11641 gtattttgtc tccacatcaa gcttcctgct gactctgtcg aaacgaaggt ccgtatagcc
    11701 gccgaggatc actatgttgt tggcctcgcc aattgaataa ccatcatact tgctgtcggc
    11761 taccagtgag ctcacccttt ctactgatac ctttgagtat ttgctgtcaa ccaggagcgc
    11821 agtcgccctc tcaacactga acatcccgac atagcgcgca tttatctcag cccagccgag
    11881 ttcataaaca gtggccttgc cgtatgctac catcagcgta ttcagaggct tgacattccc
    11941 gcgcgtgagc ttgtcaaccg tcagattgcc gtacctgaca tttgccctga caagccctga
    12001 aagctcatca ataaccgtgt tgccatactt gttggtgaca tcaaaattca ccctggcagg
    12061 catttttatt acatagttga tgctgaaact gttgtttgat cccctccagc ttatcgatga
    12121 gaattcattg ctaaagatgg tttcagcctt aaggttgcca tcttcctcgg tgaactccac
    12181 actgatcatg ccaagaatcc tcctggccct gtcggccgat gaatgtataa ccttcacggt
    12241 aacatctaca actacaaaat tcttatccca cgtctcggtg accaccgaac cgtacttatt
    12301 gataacggtc agtgtggaat tgtcaattgg agtaaactct ctgtgaaact cctttttgac
    12361 ctctgctgcc gaggcggaaa gggctaccag gcacagcaac gctgcaaaca gagtaacccg
    12421 ctgattagta aacaactttt tcatggctga ctggtttaac ggtttctgct tttatctctt
    12481 tgagctggtc aattatatag ctcatcactt ctattttggt ctggtaatga ttgatcatgg
    12541 catcaatgat ccgctggtca tccttgttga ccctcagttc cttctgcagc tcaacgtaga
    12601 ccgaatccat ggattccatc tctttcatca gagcctcttt ctgttcagga ttggacatgt
    12661 caatcaatgt gagctcgtct tccatcagtt tcacctgctg cacataatat ttttcaacct
    12721 cgcggtattc aggcgagacc tggctgagtg tcatctttgt cctcagattg ggtctgatga
    12781 agtggtcaaa ggtccacagt gaggagagtg tcaccagcag ggttaccacc gcagccctaa
    12841 gcaggtaggg aacgatgctt ctcctggctt tgccggcatg aagacgtgtg gccagcttaa
    12901 agctgaacct ctcgaaatgc ccgtctgacg gctcgctgtc atcaaactcg cttatgttct
    12961 tcctgattat gtcttctatc ttgttcatca ttcgtaaatc ttcctgtttt ttagttcgct
    13021 caccagtttc tgttttgccc ttgagagttg tgatcttgag gtactgcttg tgatgttgaa
    13081 catctctgct atctcatcgt ggtcataacc ttctatgaga tatagtgaaa gtatcacgcg
    13141 gtatccttca ggcagccgct tgatggcagc ttttacctcc tctacccgaa gcgcaagatc
    13201 ctcctggtaa gcagcttcct caccgctgtc gtcacggatt cctgcgtgat tttcaatatc
    13261 ttcaaagatc gccttccttt tccccaggac atcaagagat ctgttgacca ctatccgctt
    13321 cagccacgcg ccgaagctta ccgtccccga ataggtgttt atcttctcga aagctgagag
    13381 aaacgcctcc tgcatcacat cttcagcttc catctcatca tttacaatac gcaggctggt
    13441 attgtacatc gccttatagt aaagcttgta aacctggaat tgtgcccgct ggtcacctga
    13501 acgacacccg tcaatcaggt cctggtggat gtttttgaat gctgcttcaa ctttttccat
    13561 ctttttacaa agatccggtt taactatgtt tgatgccgca cccacgtatt tgaactcttc
    13621 aattcaatga cgtcagcgat gtccgaatgc tgcatccaag gtaaaaaaaa gctgtaagag
    13681 gatgaatatt tttttatccc tgcgtcacgg ttcctgtaac atagtcaagt tcagacccga
    13741 acaggctgtg gggaattgag taaatcagca acaggatcag cgcagcagca attgtatatg
    13801 caggtcttgc cttcctgcgg ttcatgataa ctgcaactac ccatactatg atggcaataa
    13861 gcgttttatt gtcggtcatg tcccacccga acggtatgcc ggtccaaaac tttccgaagg
    13921 catacttctg gataaccggg cccagtacca gtcctcctgc aatgagcagc cacagtgcaa
    13981 ggcggccgtg cttcctctgt gaatctcttc ccgccaaagc ggtgataccc gcaagattgg
    14041 agagcaacat tgcaaaaaac attaccagta tgtgcggaat catgatcacg gcaggtacat
    14101 tgcccttgaa cctgatgaca acgggctttt ccctgagaag cgctgtctct gaacctgatc
    14161 tccccttcag tacaatgtaa tactccagct tccctgcagc aggctgcgat gggagctcac
    14221 cggcaagctt cccgctctca tgcttcatca ccacgggtgt ccattcgtca ttcgtcctga
    14281 agcggcggaa atacaacgtt cccgaaacct cctccggaac atccagtttt atcaggcagg
    14341 gatgggtgtt tgtctgactg cgcttaagcg cgaaggagta ttcggtcccg tcaacagatg
    14401 cactggccct gtatggatgg gtgggaccgg tcatcctctg gtacatagcg gcagcgacgg
    14461 taatgattat ggctatgatc aggaaaagag agttcttcat gatgattgta cttatctcgt
    14521 tatcactgaa ttaataacag ttgcctgctc acgttcatgt cacccttccc gtgaggaggc
    14581 ttagtccgga gccaccagtc cggcccgctt gtcagtgtca ccggccgctt tcctgccgcg
    14641 aagcagtcag ctacaggttc cccagggagt cagcccggcc tgcacaacag cactaaatcc
    14701 ggaaactgaa aaccttttct cagtcaaact gaaggatcgc cttctttatg agtgatatgg
    14761 catcacgcag ttgctcttcg gtaatgatga gaggaggagc aaagcggatg atatgctggt
    14821 gtgtcggttt tgccagaacg cccagttctt tcatggcaag gcatacatcc catgcctcct
    14881 tgccgttctt aggcctgatg acgatggcat tcagcagacc cttgccgcgt accagctcaa
    14941 tcatgtctga cttcaccgct ctcatttcgt cgcgaaatat cttcccgagc ctttcagcct
    15001 tttctgcaag tttctcatca cgtatcacgt cgagggcagc aatggccacc ttgcatgcca
    15061 gcggattgcc tccgaaggtt gagccatgct caccgggctt gatggtcatc ataatctcat
    15121 catcggccag tacggcagat accggaagca caccacccga gagtgccttg cccagaatca
    15181 gaatgtcagg cctgacaccc tcatggtcac atgccaggag cttgcccgta cgggctatac
    15241 cggtctggac ctcgtctgct atgaacagca cattgtgctt tttacaaaga tcatatgcct
    15301 ttttgatata tccctcgtca ggtacataaa cacctgcttc gccctggatg ggctccacca
    15361 ggaaaccggc aacatcagga tcagacagag ccttctccag cgccgggatg tcattgtagg
    15421 ggatggttat gaagcccggc gtgaacggac cgtaatcctt gcgcgcatca ggatcggttg
    15481 acatggagac gatggtgatg gtgcggccgt ggaagttgcc ttcacagacg attatcttcg
    15541 cctggttctc ctttaccttc tttaccaggt atgcccattt gcggcagagc ttcagggcgg
    15601 tctcgtcacc ctcggcgcct gtattcatcg gcagtacctt gtcatagccg aaatattttg
    15661 ttatgtactc ttcatactcg ccaagcaccg aattatagaa tgccctggag gtcagggtga
    15721 gctttgctgc ctgtctggtc agggcatcta ttattttcgg atggcagtgt ccctggttga
    15781 ctgcggagta tgccgagaga aagtcatagt aacgcttccc ttccacgtcc cagacaaatg
    15841 gtccttcacc tctgtcaagc actaccggca ggggatgata gttatgcgct ccgtacttct
    15901 cttctctttc aatgtaatcc tgaggtttca tagctctctg ttttattctg ttgtattatg
    15961 ttatgttgta ttttttttct ggcgctggca atgataataa cctttttcga cataaagaaa
    16021 gagaggaatc agctttcttc tttgtccagc aggtcaggtc gcttctcccg ggtgatgcgg
    16081 agtgactgtt caaaacgcca cttttctatc tcaggtgtgt tgccaccggt aagaatgtca
    16141 ggcaccttcc agcccatgaa ttcagcaggt ctggtaaact gaggaggtgc aagcaatccg
    16201 tcctggaaag aatcggagag ggctgactca gcatcggaaa tggccccggg caaaagcctg
    16261 acgacagcat cggtgatgac ggcagcaggc agctcgcctc cggtaaggac ataatcaccg
    16321 attgatatct cacgcgttat cagatgatcc ctgatgcgct ggtcaatgcc cttgtaatgg
    16381 ccggcaagga tgataatgtt ctccttcatt gagagctcgt ttgccattgc ctgggtaaac
    16441 ctggcaccgt caggggaggt atatattatc tcatcataat gacgctccga tttcagatgg
    16501 gaaatacaat tgtacaccgg ctcaatcatc ataaccatcc cggcgccgcc gccaaaagga
    16561 tagtcgtcaa cactccggtg tttgtcagtg gaatagtcgc gcaggtcgat gatgtttatc
    16621 tccgcgatgc ccttctgacg tgctctgcgg atgattgact catcaaacgg actgcgcaga
    16681 agcccgggaa gtactgttat tatatcaatt cgcataaaaa tcgcgtttgt aaatctaata
    16741 ttgtttttcg tatatttgga aacgacaaac tgaaattaac tattttaaaa atttagagca
    16801 tgggaaaatt tgttatctcg aagagaaaaa atggtgagta tcaattcaat cttcttgcag
    16861 gcaatggcca ggtaattctt accagcgagg gttatgcatc aaagccttca tgcatgaatg
    16921 gtgtcaaatc agttatgaag aatgcaactg atattaagag gtttgaatca aaggttgcca
    16981 agaatggcaa gttccacttc aacctgaagg ccgccaatgg ccaggtgatt ggtacaagcg
    17041 aaatgtacga gtcagagtca agccgtgaga atggtattgc ctcagtgaag aggaacgctc
    17101 ccggtgcaac cattgttgac cagaccaagg cataatcact ctttcctttc gtagacgatt
    17161 ctaccgtcaa cgatagtata atctgattta actgacataa tcatctcctc aggagtatta
    17221 aatatatttt ctgagaggat ggttatgtct gccaaatacc ccttcctgag cattcccttt
    17281 ctgtcttcca tgaactgggc atatgctgcg cccagggtgt agtattcaat ggctttttcc
    17341 attgttattt tctccatcgg gaaccagccg tctccctctt caccctttct gtccttgcgg
    17401 gtcactgatg cgtagagacc ctccatgggg ttgaggggct caacggagta gtctgttccg
    17461 aaggccagct ttgccccgct ctctgccagg gttttccatg cgtaggccca gcggctgcgt
    17521 tcaatgccaa tgcgcttctc tgcaaaccgt ttgtcagtga tgcagtgcac cggctgcatt
    17581 gatgctatca ctcccagctc agcaaacctc ggaatgtcct cttcaataag tatctgggca
    17641 tgctccgagc ggtgccggct gtcacggatg ccgtttacct ccctcgcttt ctcaaatgca
    17701 tccagtatcc agttgttggc tttcggacca atggcatgta tccctatctg aaacccgcga
    17761 ctgtcagcgg cgagcacacg tgcctcaagc tcctcccggc tcatctgggg gaggccgctc
    17821 ttgtcaggag catcggcaaa cggctcaaac atcattgcag tggctgaacc cagcgtgcca
    17881 tccatgaaat ccttgaggta accaaacctg atccagtcgc ccgaggccag gtacttctcc
    17941 ctcagagtct catacctgtc aagacgctcc ggatcagcgg tgagcgtgcc tccgatgtct
    18001 atccggcacg aaagcttccc ggcagccatg agtgagtcat acatgtcaaa attggcactg
    18061 ccgggtatct gtatgcttgt tatgccatat ccggctgcca tctgcagagc cataagataa
    18121 ccgttccatg ccctcgcacg ctcttcctca ggtgtccgtt caacagcaat tgcaccagtc
    18181 ctgatcagtg acatggcgct ctccttgaga atacccgtag gctctccggt gaccgggtca
    18241 tgcattatct cacctccaaa aggatcaggc gtgttccggg taatgcccga ttgcctgagc
    18301 acatagctgt taaccagtgc actgtgacca tcggtacggc ggaggacaac agggttatcc
    18361 ggcgaaacgg catcaatgag ctccttcgtg ggccattcgc gggtggcgaa catctcatgc
    18421 tcccagtggc cgccatagat cagctgaccc ttctgcgagc gggctacctg ctccttgacc
    18481 ttgtttgtaa tcactgaagg atcagtgaca tagcgaagtt cgatataatc gggatcaaca
    18541 ggcccgaagt gcgcatgtgc gtcattgaaa cccgggagaa cagcccggcc gccggcatct
    18601 ataaccctcg taacctgcgg atcaatatac ctgccaatct ccctgtcact ccctgctgca
    18661 agtatcctgt tccccttcac ggcaacagcc tctgcccggg gattaagcga atcagccgta
    18721 aaaacaacac ctccggtgat taccagatca gccttttctt ttttcgtgca tcccaaccca
    18781 acagtcagta atagcgcaat aatcattaat attgtctgtc tcatggaaac gatttttata
    18841 atactggtgc aatataagaa aataggcatt attttagccg ctcataaacc tgtgaacatt
    18901 aactccaatc tctgatcaaa tgaagaacaa gatcattcca ccaggatatc tctattcaaa
    18961 gagatatacg aattatgtat ttatcctgct cttccttctt tatatgtttg actacataga
    19021 caggatgata gttacatcca tgttcgttca tattgagcag gaccttggta tcactcacac
    19081 ccagagcgga ttgctggtct cagccgtata ttggtccata gtgctgctca cattccccgt
    19141 ttcaattctg gttgaccggt ggaaccgcac caaaaccatc ggaataatgg ctgcattgtg
    19201 gagcatcgcc actgctctct gcgcacttac cgggaacctg atacaactgg tgtttgccag
    19261 ggcattgatc ggcgtagggg aggcaggata tgcacccgga gggactgctg ttatttcagg
    19321 tctctaccct gaagacaaga gatcaaagat gatgggattg tggaatgctt ccataccgct
    19381 tggcacggca ataggcgttc tcctcggggg aatcatcgcg gcaaggctgg gctggaagca
    19441 tgcattcgga atagtggccc tgccagggtt tattgttgcc gtcctctttt tctttgtcaa
    19501 ggactataaa actgttgacc tttcatttac aaacagcaat aacgtgaagg tcaaaatgaa
    19561 gaaaaaggac atggccaggg agttcctttc cagaccttca ctcatcttta catattttgg
    19621 ctttgctgct gtggtctttg tgacaacttc cctgatcaca tggctctcca catacttcaa
    19681 tgtcacccgc gggctctctt cagaaaaggc cggaacaatg gccagtgctg tcatgttgct
    19741 ggcaatcgtg ggggcgccgc tgggaggcat tatcactgac cgctggcgca aatcaaatat
    19801 ccgggcacgc ctgctctttc ctacaatatc atcccttctt acggcactct tcctgctcgt
    19861 tgccctatgg ctgaccagcg gtacactgca gtatgttttc ttcctcctga tgggagtcac
    19921 agtggcagcc ttcataccgg ctgccgcagc tgtcacacag gacgtgattc acgcaggaat
    19981 gagggcgatg tcttatgccg tggcggttgt ggtacagaat cttctgggtg catcaacggc
    20041 accccttgta ataggaagac tatatgacct ctatgacatc gaaacagccc tggcgattct
    20101 gccgttcatg ctgattctgg gtgcatttct tttctatatg ggatcacgtt tctatgtgag
    20161 ggacctgaac aaaactgcca aaataaaact ggaagccgaa acaaacaaca gaatgagatg
    20221 aaagaactat tgcggaataa atacttcaaa ttcggcgttg ccggagtact ctacatcctg
    20281 tgggtaatat gggtgggcaa ctactggctg ctgctcggat tgccgatagt attcgacata
    20341 tatgtatcca aaaaggtcaa ttgggcattc tggaagaaac gtcatggcaa aaaccatgta
    20401 gttgttgaat ggcttgatgc attgatcttt gcagtgattg cagtgtcact tatcaatatc
    20461 ttcctcttcc agaactatcg cattccgaca ccctccatgg agaagactct gctggtgggt
    20521 gatcacctgg ctgtaagcaa ggtggcatac ggcccccgca tgcccaatac accgctggta
    20581 tttcctttta cccagcatac aatgcccttc accggcggca gggtgaaatc atggtctgac
    20641 ctgattcact ggaagtataa tagactcaag ggattcgggc atgtcaaaaa caatgatatc
    20701 gtcgtattta atttcccggc aggagatact gtctgcctgg aacagcaggc agtaagctac
    20761 tatgagattc ttcgcgaacg gattgagaca atcactgccg aggatatcag ggcaggcaga
    20821 cctttgaaag gcaaagatga gtattattca attgcaaggc agatgatctg gaatgagtac
    20881 acggtcatct acaggcctgt cgaccggcgt gacaattatg tgaagaggtg cgtcggcata
    20941 cccggcgaca ctgtcctgat aaaaggaggg aagctgttca tcaacggtca tgagaacact
    21001 gaatacgaga cccagcagac atcctattac gtgcgtacga atggtacccg tatcaatccc
    21061 aaggctttcg aaagaatggg ggtgtcaaag tctgaccagt ctcttacgat ttcaggaagt
    21121 ggctacaagc tgccgctgac ccggaagaat gcagagacca tctcgggatt tgccaatgta
    21181 acgggcattg aagctgacta ccgccggccc ggagaataca ttgccgctgt ctttccgcat
    21241 gatccggcgt accgctggaa cgaggataac ttcggcccgc tatggatacc caaaaaggga
    21301 actacggtga agcttgacat gaccaacatt aacttctata catccattat cgatatctac
    21361 gaggagaatg acctggagat acgtgataca gtgatatata taaacggcaa gcctgcagac
    21421 agttatacct tcaggatgga ttactactgg atgatgggcg ataacaggca tgactcggct
    21481 gactcacgct tctgggggtt cgtccctgag gatcacatca tcggtcgccc tgtgcgcgtc
    21541 tggctctcta ttgacaagga ggcgcaggga ttaaaaaaga taagattcaa caggttcttc
    21601 aagggagcca aaaggtgata aagagagggg tctgagcccc ctgactgcat ggcaggataa
    21661 tccggataat agataacgta aagaggggtc tcagacccct ctctctttac tcttttactt
    21721 tgtccactat cgccttgaag gcttcaggat ggttcatcgc caggtcagcg agggtcttgc
    21781 ggttaaggga aattcccttc ttctcaagcc cgccgataaa ttgcgagtaa ctcattccgt
    21841 gaagacgtac tcccgcgttg atcctctgga tccagagtgc gcggaactgc cccttcttgt
    21901 tcttgcggtc acggtacgca taggtaagac ccttctcaag cacgttcttg gcaacggtgt
    21961 aaacattggc atttttgcca aagtaccctt tggtctgctt gagaatcttt tttcttcttg
    22021 ccttcgatgc tggtgcgttt gttgctcttg gcattttgtt tctctttttc ggatgtaagc
    22081 gcctctttaa ggtcttttat gcttcacatc ccggttaata attctgttaa ttaactctcc
    22141 gtcaggtggt aaacactact tcagccccaa cagaagcttt acgttcttct gatctgcctt
    22201 gtcaaccagt ccgctatagg taagattcct cttgcgtttt gttgacttct tggtaagaat
    22261 gtggctcttg taggcatgct tacgcttgat tttacctgaa ccggtcaaag aaaatctctt
    22321 ctttgcgccc ggatttgttt tcacttttgg catattatat atatgtgtat aaaaaaatct
    22381 acttcttttt ctttggcgag aggattatta tcattctctt tccttccagt cttggtgcct
    22441 ggtcaacctt tccgtactca tcaagatcac tcacaaacct tgcaagaagc ttttctccct
    22501 ggtccttgaa gagaatagac cttcccttga aaaagacata tgctcttact ttggcccctt
    22561 cttcgaggaa gttgatcgcg tgcttgagct tgaagttgta atcgtgatca tcggtgttcg
    22621 gcccgaaacg aatctctttt acaacaatcc tggctgcatt tgctttgatc tccttctgct
    22681 tcttcttctg ctggtagagc agtttctggt aatcaacaat cttgcagacg ggcgggtcag
    22741 catttggaga tatctcaaca agatcaagct ccatttcgtc tgccagcctg agagcatcag
    22801 aaatactcat gattttggtt tctacgttct ctccgacgac tctaaccgtc ctggctgtga
    22861 tcctgttgtt aatccggtgt tccggttcgg gttttcttcc cggcctgggc ctggaaaagg
    22921 ttggtcctgc tatagttttg tcctccagtt aagttaataa tatctttatt aatatgaatc
    22981 ttaaacacca gtctctctct ttatctcctc tttcacaagg ttgacgaact cctcgggttt
    23041 cataactccc ctgtcacctt ctccctgctt tcttacggag actgtacccg ccaattcctc
    23101 tttttcacca atcacaagaa ggtagggaat tctctttaac tcattatccc ttatcttctt
    23161 acctatcttc tcattcctgt cgtcaatcag ggtgcgaata tcgtaattat taaggaaatt
    23221 taaaacagtt tttgcataat cattgaattt ttcactgatg ggcactatta cggcctgatc
    23281 agtagtgagc cagacaggaa aacgtccggc agtgttttca atgagtactg caacgaaacg
    23341 ttccattgaa ccgaaaattg ccctgtgaat catcaccggc cggtgcttct ggttgtcact
    23401 gcccgtgtat tcaagcccga accgctccgg caggttgtag tctacctgta tggtcccaag
    23461 ttgccacttg cggccgatgg catcccttac catgaaatca agcttgggcc cgtaaaaagc
    23521 tgcctcaccc actgccaccg tggtaataag gttcatttcc cctgcagctt cgataattgc
    23581 cttctctgcc ttctcccagt tctcgtcact gccaatgtac atctcacggt tatccggatc
    23641 cctgagcgat atctgtgccg tgaattcatt gaaaccggca attttgaaga tatactgtac
    23701 caggtcaata atgctcttga actcctcctt gagctgttca ggggcacaga aatggtgagc
    23761 gtcatcctgg gtgaagctgc gcacacgggt cagtccgtgc agctcaccac tctgctcgta
    23821 ccggtacacc gttccaaact cagccattct taccgggagt tcccggtagg agtggggagt
    23881 ggcgttgaaa atctcacaat gatgaggaca gttcattggc ttgagcataa agacctcacc
    23941 atcctgcggg gtggtgatgg gcctgaatga gtcagccccg tacttctcaa agtgacctga
    24001 agtcttatag agctcaatgt tgcctatatg aggtgtggag actattgtat atcccctttc
    24061 ccgaaggact tttgtcatga aatcaatcag cctctgtttc atgtcagctc ctttgggaag
    24121 ccatagcggc agtcccatac ccaccttcgc cgagaaggca aacagctcca gttccctgcc
    24181 tatacggcga tgatcacgct tacgcgcctc ctcgagcatt gtcatgtact catcaagcat
    24241 cttctgcttc gggaaggtga tgccgtaaat gcgggtcatc atcttccgct tttcatcgcc
    24301 acgccagtat gcaccggcaa ggctgagaac ctttatcgcc ctgattgatg aagtatcagg
    24361 caggtgcgga ccacggcaca gatcggtgaa gttgccctgc cggtaaagag ttattgtccc
    24421 gtcctcaagc tcggagataa gctcggtctt gtattcatca cccttggcag tgaagagttc
    24481 cattgcatcc ttcttgctaa tctccatacg aactatcggg ctcttccctg ctgcaagctc
    24541 cttcatcttt tcttcgataa gcggcaggtc ggaatccgtt ataaccttgc cgtcaccggg
    24601 atcaacatca tagtaaaaac cgttctctat ggccggaccg ataccaaatt tggttccggg
    24661 gaaaagagcc tcgatggctt ctgccataag gtgcgctgac gaatgccaga atgcatgctt
    24721 tgcctcggta tcatcccatt tgtgaaacct gacggtggca ttgccttcaa caggctgcga
    24781 aagatcgcga acttcaccgt tgactgttgc tgcataaaca tcggcagcaa gacccggact
    24841 gattgatttg gcaatttcat gactggttat accctgtctg tactccctta cagagccgtc
    24901 aggaaaggtt atgctgatca tcggtttcat actttttaac agtccgcaaa ggtaatattt
    24961 tttactgtaa ggtgttcccc tgtggtttcg aggtaaaacg gtcctcattt tagaaattgt
    25021 taattggcac agattttgga tttagtataa cagcaccaaa aacagcagtc atgaaaagag
    25081 ttctgatctt aaccgcagca gcagcattga tgatgctgaa ctcatgtgac atacagcccg
    25141 aatctcattt cttcgcagat aagataaagg ctgaaatagg agaggatata ttcttcttca
    25201 acaggtcata caacgcaact gattacgaat gggactttgg agacggcact ttctcaaacg
    25261 cttacgaacc ttcccatgca tacaacggat ccgggatttt tacagtgatc ctgaccgcat
    25321 attcaaaatc gggcagcatt gacaaatctt accttgatat cgagatcatt tccccaacaa
    25381 tgcttgaaat agaggtgctt gaatactacg accagtaccc ggtttcagga gcaagcgtga
    25441 tactctatcc tactgagaaa gactgggaca atgagaccaa tgccatagtg gaggggttca
    25501 ccaacgcctc cgggaaggtg gtattcacta acctgcagcc cagggtctac tttgttgatg
    25561 tctggcattc aacccacaat aattacaccc tgagggatga ggatacaggc ttcatcagga
    25621 cagatcagct ggaaaagaat cagctcaaca aattcatcgc atgggttgac tataccggta
    25681 ccaagggagc cacagcccgc gacaggaaac agctggttcc gctcaagagc aggacggttt
    25741 cagcaaccgt aaaaaagtaa ttcacagaga taaacaaatt aatttgacgc atggctccat
    25801 ttgataaatt tccgtaagtt tgtgagttca caaacctaat cggaaagaat ctcatggata
    25861 caaaatttgc gcttcatgga atagactacc tgataatggt ggtctatttc gtttttgtgc
    25921 tgggtattgg atgggccctc aggaagtaca tgaagaatgc ttcggcattt cttgaggccg
    25981 ggcgctcgat accagcctgg gttgccggtc tggcatttat ctccacgaac ctgggagccc
    26041 ttgaggtgat ggggatgacc ggttccggcc tgaaatacgg gatgctcacc acccactttt
    26101 actggatagg cgccatccct gcaatggttt tcctggccct cttcatgatg cgtttctatt
    26161 atggttcaaa ggccaggtca gttccggaat atctcaagct tcgcttcaac gaaaagacac
    26221 gtgccctgaa cgcattcctt tttgcgctta tgacggtgct cgcatcaggt atctcaatgt
    26281 acgcgctggc tatcctgcta gagaatgtgc tcggatggaa cttcaaccta tccctccttg
    26341 cttcggcaac catagtgctg ggctacacat acctcggagg gctctcatct gcaatataca
    26401 acgaggtgct gcagttcttc atcatcatta taggtctcct tccaatgacg atcctggtgt
    26461 tggttgacgt gggaggctac ggtggaatgc aggcgagcct cgccccgata gtagagggaa
    26521 agggattcgc cccggacaca tggaccacca cctggaagta tacggccaat gccagcgcca
    26581 acccgatggg agtggagtgg tatggcatac tggcaggact gggattcgtc ctttcgttcg
    26641 gatactggtg taccaatttc cttatcgtcc agagggcaat ggctgctgat tcgatgactt
    26701 cagcccgcaa ggtccctctc atcggtgcaa ttccgaagat gtttatcccc ttccttatca
    26761 ttatccccgg tattgccgcc ctggtgctgc tcaatgaccc cggcaaggga ttcggcctgc
    26821 cgctgaatga agcaggccag ccgatatatg actatactat cattatgctt ctcaagcagt
    26881 atctgcccgc cggtgtgctt ggctttggca taaccgcact ccttgcatca ttcatgtcag
    26941 ggatggcagg caacgtctcg gcattcaata cagtctggac ctatgacatc taccagagct
    27001 acatccggcc tgcaacaggt gacaaggagt ctgatgaccg ccactacctc aaggtcggta
    27061 gaatggctac aatcattggt gtgctggtga gcattgctgc cgcatatatt gccagcaagt
    27121 tcggaaacat aatggatttc ctgcagactg tcttctcaat gataaacgcc ccgctctttg
    27181 cggtgatatt cctcgggatg ttctggaaac gctcaacagg aaatgcagca ttcacaggtc
    27241 tgctggcggg attcctcatt gcactgctcc accacggact taccatgcct gagggcgcaa
    27301 caacacttgt caagggcgga tggatcggag tggtgcatac ataccctgtt gaaatggcac
    27361 agaacttctg gacagccatc ttcgctttca ctatggccgc actgctgacc gtaatcatct
    27421 cactgctctc gaaacgcgac aagagcgacg cggagctgaa cggactcgtt tactccatga
    27481 ccccgaagct tgacgacagc ggagtgatat ggtacaagag accctggttc cttgcactca
    27541 tcgtgggcgt cataatgatc gtgctgtcaa taatgttctg gtaaccttaa aaaatatcaa
    27601 ttatgctcga tataagattt ccaattggac tgatgttctc aatagtagga ctcatcatta
    27661 ccatctacgg gcttgccaca atgggacagg atgcaatgta tgtcaaatca ctcaatgtga
    27721 atgtcaacct gctttccggg ataggctgcc ttatcttcgg cctgctgatg ctgctcttct
    27781 ctgatcctgt gaggaaacgg aataaaaagt aacgaaaatg aaggtgcctc agaaggggca
    27841 cctttttttt gccggcagga atgactgacc gggcttaccg gtaatacaag gcttaccgtt
    27901 aatacaggct taccgttaat accaggatta ccgttaatac cgggattacc aggattacca
    27961 ggattaccag gattacaagg ttaccaggat taccatgatt accgggattc ctcccgtatg
    28021 atctacaaac tatcagagaa gagcgctgcc ggcagggaac gcatattgta ccttgaggcc
    28081 atcacctcac cgtaggcacc ggcagagcga atgacaatca ggtcgccgcg ccgtgtttcc
    28141 ggaagctgga tattcctgcc aaaggtgtca gtggtctcgc agactggtcc cacgacggta
    28201 tattttttca ccggaccggt ggaagtgaga tttttgatcg aatgtctggc atggtagagg
    28261 gcaggcctga tcagctcggt catgccggca tccaggatga caaatgtctc caaggcgcct
    28321 ggcttgacaa acagtaccct gcttacaaga gagccacaat ttgctgtcag cgaccgaccc
    28381 agttcaaagg atatggtgct gcgtatcctg ctgtcaagag aggtggagaa aatgttaaaa
    28441 tagtccgcga acggcgggat gcggtccggg tcattatagc tgactccaag tcctccgccc
    28501 acgttaatgt cttcaggccc gaagcccctg ccggtcagcc actgccataa ctcgtttatc
    28561 cttgtacaga ggttgcggta gacattcagg tcggtgatct gtgatccgat atgaaaatga
    28621 attcccctgt aacggatgtt ctgaagctcc ttcagccggt caagcacatt atcaagatcg
    28681 ggtaatctta ttccaaactt gttctcatct gtgccggtgg tgataggctt gatggtgtgg
    28741 gcctgcacgt tgggattgat gcggagggct accggggcct tttttcccat cctgcctgct
    28801 attgagtcaa tgacattgag ttcttctgcc gattctgcat tgaaacactt gatgcccagc
    28861 cggagagcct tctctatctc agtatcgctc ttgcctacac ctgcaaacac cgtctcactg
    28921 accggaaatc cgcatgcaat ggcatgctca acctcgttgc cgctgacaca gtcagctccg
    28981 aatccctgct ttgcgatgat cttcattatc acaggattga agttggcttt cacggcatag
    29041 tgaacatgaa aacccctgcc ggcagcttca cgtgatgcta cggaaagagt ctcgcggaga
    29101 atatccagat catagtaata aaaaggggtt tgatgcttcc ccagcttttc agtaatctcc
    29161 ctggtaatca tgtttcttat gcaaataact tttcattcag tgccctgagg gcggtaatct
    29221 tgtcggttgt gttgataagg atcgagacgc tgtgatcact gccaccgtag gagatcatct
    29281 tgaccggtat ctcgttaagt gcttcaaata tctccggggc cgagcccaca cgctcaggac
    29341 ggaagtcgcc caccacacat attattgtct ggttgtgctc cgtcttcacc tgcccgatgg
    29401 agctgagaga tttcacaatc tcttcaagat gagtggtgtc ttcaatggtc agtgataatg
    29461 agacttcgga ggtggttatc atgtcaatgg gagtcctgaa ggtttcgaat acctcgaaga
    29521 tcctcctcat gaaaccgtag gccatcagca ttctgccgga gtgaatgcgt attgaagtta
    29581 tgccgtcttt ggcagccact gcggtatagt cttccaggtt cgtttccgag gtgatgaggg
    29641 taccctcatc ggcaggttcc attgtgtttt tcagccgtac cgggatgttt ttcatcctcg
    29701 ccgggttgat gctcaacgga tggaggatct tcgctccgaa aaaagccagt tcagccgctt
    29761 cgttgaatga gagcctcctt acaaccctgg tgttctccac atacctcgga tcattgttgt
    29821 gtagcccgct tatgtctgtc catatctgta cctcttcggc gccgatggca gctcctatga
    29881 tcgtggcgct gtaatcgctt ccgccacggc ggaggttgtc agtctcaccg aagacgttgc
    29941 ggcagataaa gccctgggtg ataatgatct tttcatccct gacggccgaa agcccacgct
    30001 tcagattctc actgatataa aagtaatccg gctcaccgtc cttgtctacc ctcatgaaat
    30061 ccagggcagg caacaggagt gagggtatgc cctgctcggt cagcaggaag tgcagcaggg
    30121 ccgttgatat gagctccccg cgtgcaagga tcatcttgtc ggttcccctg gcaggttccc
    30181 ctgatatgag cgaggcaatg aaatcaaaat gctcatcagc aaggtcatgt gccctctttg
    30241 accactcttt agaggcaaaa agatcagaaa tgacggcatg gtacttcgag cgcagctggc
    30301 cggtcatccg ctctgctccg gatttgtcgc ccgatcccag gagcccggaa atctctgcca
    30361 gggaactggt tgttcctgcc atggcagaaa gaacaacaat ctttagcccg ccgtcattta
    30421 taaggggaat cagtgccctg agccgttcag gacttcctgc cgaagtgccg ccaaatttgt
    30481 aaaccttcat ctctttttct ctccgtacga tgctgcccgt aatgctgttg caccatattt
    30541 cgggcatcaa agataagtaa aagaggaatc tgtaaatatt tttttctttt ctgtaaagtg
    30601 aatcacttga caaaaaaata gttatttttg cagccgcatt gatggattaa ggatggctgg
    30661 cgtcaatttt tcagacagtc tccctgatcc ggcaagtagc ccagatggcg aaattggtag
    30721 acgcgctagt ttcagggact agtgtcagca atgatgtgca ggttcg
//
LOCUS       DAOPBK010000003        30291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_1655_length_30291_cov_5.774176, whole genome shotgun sequence.
ACCESSION   DAOPBK010000003 DAOPBK010000000
VERSION     DAOPBK010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30291
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1655_length_30291_cov_5.774176"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..914)
                     /locus_tag="PLA17_00545"
     CDS             complement(66..914)
                     /locus_tag="PLA17_00545"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83929.1"
                     /translation="MKVIEPKDLEPLSFLFRGAKGQRFAEWLIRSLAIDKVNRAYDKL
                     GAWSGSEFTTRLLKDIGVSYVVGNPERLGDLPAGAFITISNHPYGGLDGIITIDMMAR
                     IRPDYKFIVNELLSLIKTLEENFIPVVPKGDKQTTIKATSIKGIREAIEHLGAGHPLG
                     FFPSGAVSDFSLRDMKVRDRPWQESILHLIHSVKVPVLPIRFFDMNSAFFYFLGLINW
                     RVRVLRLPSEMFNKRGSQQRIGIGELISVEEQSRFPDPLSLGESLRKKVYEMPLPEKF
                     VHGNSV"
     gene            complement(1053..1241)
                     /locus_tag="PLA17_00550"
     CDS             complement(1053..1241)
                     /locus_tag="PLA17_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83930.1"
                     /translation="MRCKTAYEKHKARARFIHQASVEPVIRHLKSDYRMPGNYLKGVE
                     GDMINTIMAVVAFNMMKR"
     gene            1492..3060
                     /locus_tag="PLA17_00555"
     CDS             1492..3060
                     /locus_tag="PLA17_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPK83931.1"
                     /translation="MKKLCLVMLTIGLFSCQSNPTTGPADLSRNTIIPKPVSVTSADG
                     TFILTSRSDIFVAEGSPEEVAVIGNYLAERLNPSTGLGLRVITDGRKPGKGDILLAIN
                     EGDTELGEEGYELVITGKVMTLTAVRPAGLFRGIQTIRQLLPPAIESATIQPGPWVIP
                     AGTIRDWPEYGHRGEMLDVARHFFGPEDVKRFIDHLALYKMNVFHLHLSDDQGWRIEI
                     KSWPNLTTHGGKTEVGGGEGGFFTQEEYSDIVKYAEERYITIIPEIDMPGHTNAALSS
                     YPELNSDGKAKEPYTGTKVGFSSLDTRSEITYKFVDDVVREIAALTPGQYFHIGGDES
                     HSTKIDDYIYFVNRVQDIVESHGKSVTGWDEIAHATLKPDVVVQYWARPANAVKGIAQ
                     GAKVLMSPCQHAYTDMKYDSTTVLGLSWAAYIEVDDAYNWDPATLVPEIKRENIVGVQ
                     APLWAETISTFDEAEYLIFPRLPGYAEIGWTPAEMRSWDEYRVRLAAHGKRMETLGIG
                     FYRSPLIDWDQPGL"
     gene            3111..4247
                     /locus_tag="PLA17_00560"
     CDS             3111..4247
                     /locus_tag="PLA17_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPK83932.1"
                     /translation="MEFLLDGITNVTWQQAVMWVVGALLIYLAISKELEPTLLLPMGF
                     GAILVNLPLSGALNQTLSGIGEVTGILDWLFKVGIEASEAMPLLLFIGIGAMIDFGPL
                     LSNPRLLLFGAAAQFGIFVTILASVLLGFELRDAASIGIIGAADGPTSILVSQVLKSD
                     YIGPIAIAAYSYMALVPIIQPFAIKMCTTEKERKIRMSYNPKSVSRMTRILFPIAVTI
                     IAGLVAPASIALVGFLMFGNLIRECGVLRSLSESAQKELVNLITLLLGITIASTMQAE
                     KFVALNTVKIMGLGLMAFVFDTVAGASFAKLMNVFLKEKVNPMVGAAGISAFPMSSRV
                     IQRLGLEADPTNHLLMHAAGANVSGQIASVIAGGVIIGLVPGLL"
     gene            4260..4400
                     /locus_tag="PLA17_00565"
     CDS             4260..4400
                     /locus_tag="PLA17_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83933.1"
                     /translation="MNPDIIKSLDLMGKGMTAIFVVIIVIYLSVNLMLRLTESKKKET
                     KE"
     gene            4552..4950
                     /locus_tag="PLA17_00570"
     CDS             4552..4950
                     /locus_tag="PLA17_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83934.1"
                     /translation="MKKALLLILVAIMASCSQMRKGSSATPKDELAITRKYVGDFIEY
                     RHTGPENYDGPNMIWIRTTMEDQYGIISAYGRRCDFTSGDRLYLRKKFYSPGIIAGYW
                     VYVIENDESVSYRATDLQHDREVYIKSWFE"
     gene            5110..5670
                     /locus_tag="PLA17_00575"
     CDS             5110..5670
                     /locus_tag="PLA17_00575"
                     /inference="COORDINATES: protein motif:HMM:NF021952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HPK83935.1"
                     /translation="MIQRDNTLYFIALIPGKELRGRVRALKERMKADYNAGHALKSPA
                     HITLQMPFKRNSRDEDSISALLEKFSSEERPFTVRLDGFGCFAPRVIFIRVREHEPVK
                     ALHSRLKVKLSEELGFSESEIMNDVQPHITVATRDLTKEAFREAWPEFQNEEFRGTFE
                     VHSLFLLKHNGKSWDILKEFPFRKNG"
     gene            5663..8578
                     /locus_tag="PLA17_00580"
     CDS             5663..8578
                     /locus_tag="PLA17_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPK83936.1"
                     /translation="MDRRRFISGSALAAAGWLAASRLRAVPWSPDGGRVPMPASSIPG
                     SVTLAGNGLWESFRDPAGHYHPFVRWWWNGNKIEAGELIRELHLLKAAGIGGVEINPV
                     AFPSRFDGDDLGKPSLEWPGEEWCNMLNIVFAEAKRLDMTCDLIVGSGWPFGAENTAE
                     EERSQVVVVAVRKVSGPSELNISREEIFMEAGPAINNPWPGKKYSLLSMMLTPDPLTS
                     AEEITDLSDRIGEEFFDLFIPEGNHALYALVKVTGFMQVINGAPGASGPVIDHFNSDA
                     VNRFLNRMSDSIEPVTGPLSDHLRALFTDSMELEGSNWTGDMREQFFKRRGYDIFPFL
                     PFVLFRTGSMGNVTDFRYGVEYGPAFLEMIRRMRFDFETTKAELLEERFIQSFTGWCR
                     DLGVRSRMQAYGRGFFPLESSMPIDIPEGESWTMNWLKHRIGEEMPEHDYRRGRAYTM
                     VNKYVSSAAHLTGKRVISCEEMTDTYTVFNTSLENLKVGSDQSIISGVTHSVFHGFNY
                     SPPEAPWPGWIRYGCYYNENNNWWPHFHLLNQYKARLSALLQNCDMYADIALLPPMAD
                     MWSLIGMQNEPFPSVLHAEHLSLVWEAIAKNGSGCDYISERILRDATVENKKLCYGPR
                     KYNSLFLVSVESLSPDSARKLLEFVRAGGRVFCVETVPSKSTGWSNHERNDGEVGLIV
                     EKMKAYPHRFICLDKPEKDIIGWYRSVQERYRINPWIRIDKPDTFLMQVRYSADDGSG
                     YIFIVNSHLHESRTTRVRFSAGLTRRRHCSVWNPETGGRKRVKPDPDGSLLLDLGPAE
                     SLLFAFDREEAEEEWRPLPSTGSDMHLLDKGWSAEFRHCRDGSVKEVMMDRLTDLKEM
                     PEFVYFSGRVIYRNRLECTDTAGMVLNLGKVYGTSELRINGVSCAVKWYGRRIFSIEE
                     YLKPGMNTVEVEVTTSMGNYMKSLTDNPVAQYWTNAGTKDQPLQSMGITGPVSCYRR"
     gene            8677..10152
                     /locus_tag="PLA17_00585"
     CDS             8677..10152
                     /locus_tag="PLA17_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HPK83937.1"
                     /translation="MIKKGTILLLAALVAVSFPLHSKDYNASMFGAKSNGTTLNTSSI
                     QKGIDYISENGGGRLVFYVGRYLTGSIYLKSNVTIHLEEGAILVGSVNPFDYDRNFNW
                     TALIFALDQENIGITGKGVIDGQGFQVATNLVDMIHKGVVKDPLKYDRPNETIRPQNI
                     YFRGCRNIVVRDINLRDPGSWNQQYDQCRNLVIDNITVDSKSYWNNDGVDIVDCDSVV
                     VSNSYFDAADDGICLKSHSPDFVCQNVYIHNNTVRTSANGIKFGTVGHGGFRNVRIIN
                     NVVFDTYRSAITFAAVDGGFVENIVVDSLRSLNTGNVIFLRIGERRAGKKGRMNGVSI
                     SNVYAEVPATKPDAGYNYEGPVEDLPRNISPSSIVGMPDAMIENIALKNIEIHYPGGG
                     NPHYAKVGLDELDKVPELGANYPEFSMFKELPAWGFYIRHARGITMENVKLVCGKKDY
                     RTAVVLDDVHGATFSKLQVTEPGSRKESIFSHNSTEIVNRK"
     assembly_gap    10349..10448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10454..13258)
                     /locus_tag="PLA17_00590"
     CDS             complement(10454..13258)
                     /locus_tag="PLA17_00590"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPK83938.1"
                     /translation="MKRILAIAVLAAVSHLIAAQASPDKNVPADPAYRIGKLENGLTY
                     YIRHNTEPAGRASYYIIQNVGAILEQDDQNGLAHFLEHMAFNGTAHFPGKTLLSTLEK
                     HGVAFGRNINAYTSFDETVYNISDVPVDKPGLIDTCLMILADWSDFLTLDEEEIDKER
                     GVILEEWRSRRNAQWRMLTQMLPVIYEGSMYAKRDIIGDTAVIKNFDPEALRAFYHDW
                     YRTDLQAIAVVGDFDVDDVEGKIKRIFSPIPAIENPIPRPENLLTAKKGTKYLLVTDP
                     EAPGTEVGLMIIDREPDKRERNLDYIRDGYVTSLMNMMMSKRFSEIVQKGTPPFVSGG
                     LRYSSDLARNYNSLTLSVYANEGEEAKGFEVACTEMERARRHGFTEGELNRAKAEMLS
                     TFENRYKQKDKISNDSWASMIKNHFLTGEPIPSLDLQYDYYKNILPGITVKEVNSRLQ
                     ELVKDDNRFIYVQGPDDREYMTEAGAVAIMEKVAAAEIEPYEDITGGTDLISEELEGA
                     EIVKTVSLPQFAATEWTLSNGARVVFRHADYEKDNVTISGYAFGGSSFYPDSLEPALN
                     LFPQVISMYGAGEFDNVALTKMLAGKKASVSLGIQETRQTVSGSSTPKDFETMMQLLY
                     LRFARPNFNREAYDAIIGRFTAVITMMQKNPDKILSDSLSMNMTNYHPRTFLLTPESM
                     KRIKYDDINYIYRTAFDDASAFTFFITGNIDEAVAREMARIYIGSLPSKYQSETYRNL
                     GMDQPKGTVKKDIFIPLTVPKATIVINYSADAAYNPSGYLSMDVLKGILDLVYTEKVR
                     EEEGGTYGVSLNAGSQKRPVEKATLLIAFECDPERASELKNIIYSELEAIAKNGPRQV
                     DLDKTVSNLLKTREEAIQHNSYWANAVSSYYLNGFDINDSRNFADILNKLTVKKMKKV
                     TRKFLSKADHLELVFLPEKK"
     gene            complement(13367..13981)
                     /locus_tag="PLA17_00595"
     CDS             complement(13367..13981)
                     /locus_tag="PLA17_00595"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPK83939.1"
                     /translation="MHIAIAGNIGSGKTTLAGLLAKHYGWQAHYEDVDDNPYLNDFYE
                     DMQRWSFNLQIYFLNSRFSQILEIKKAEQTIVQDRTIYEDAYIFAPNLHSMGLMSTRD
                     FENYFTLFKLMSSLVEPPDLLLYLRASVPTLVNQIQKRGREYESSIRLDYLKRLNERY
                     EAWIESYKLGRLLILEADYYDFPENNEHLSEVIDRINAELHGLF"
     gene            complement(14059..15573)
                     /locus_tag="PLA17_00600"
     CDS             complement(14059..15573)
                     /locus_tag="PLA17_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPK83940.1"
                     /translation="MPFIPSVIKWINTKRLSQIELFRKYPAETQQEVLTRLVSLSRDT
                     EWGREHGYSSVTTHEQFCRNVAVQSYEDVIPWIERLRAGEKNVLWPGEVKWFAKSSGT
                     TSTKSKFIPITHESLEETHYRGAKDCIVLYTSLNPETRIFLGKGLTLGGSHQINSFSN
                     NSLFGDLSAILIENAPAYAELIRTPPARIALIEDFEEKMRMITEKTVDMNITSLSGVP
                     SWYLVLIRHILETTGKANLHEVWPNLEVFFHGGVSFAPYREQYRKLLPDPKMHYMETY
                     NASEGFFAIQDDLTDSSMLLMLDYGVYYEFIPADEFGTDNPRTLSIGEVERGVNYAMV
                     ISTDGGLWRYAIGDTVEFTSLYPHKMKITGRTKFFINAFGEELIIDNAEKALEKACSG
                     THAHISEYTAGPVYMGDESRGAHEWVIEFDREPDDPEHFIEILDTTLKALNSDYEAKR
                     YKDLTLARPRMIAVPRGTFYKWFRERDKLGGQNKMPRLSNNREIVESVLKTAGL"
     gene            complement(15614..16336)
                     /gene="recO"
                     /locus_tag="PLA17_00605"
     CDS             complement(15614..16336)
                     /gene="recO"
                     /locus_tag="PLA17_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPK83941.1"
                     /translation="MLTRTKAILLHHVRYSDNSLIARFYTQEYGRLSVMVKGISSKRG
                     NARFNYFQPLNIFNTELYYYENRELHNLKEMTLAYIPKRIHGDIHRTTIAMFISELLY
                     NVIREEDVNKLLFDFIESSVISLDEMTSGISNFHLWFLVAFTDFTGIGPTGTDMTACY
                     FDMLTGQFTPLRPMHTDYLNPAGARVLNLLLQMPVEELASLKLSGEERTELLAGMLKY
                     YRLHLPGIREMRSLQVLRDIFR"
     gene            16440..17834
                     /locus_tag="PLA17_00610"
     CDS             16440..17834
                     /locus_tag="PLA17_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPK83942.1"
                     /translation="MESESKERLLRRIARLVRQNKELEEQVKKLEREKERLLETTEKY
                     RLLVENNELKGVRVEEEDKSLKFNMVTVLFASIQGFPNMTEEIDPALMMDELDEIFFE
                     FDRIIRKYHVEKIRTIGDTYMCAGGIPAKNITNPVEVVMAAVELRQFLRDFEAARRGE
                     KRIWNLKIGIHTGPVSATVSGKSRVSYDIKGRAVNTASRIQGVSESDSILISVMTYEL
                     IKEFFNCDYYGKLPVKYMGNIQMYQVAGLLPELAADSNGAVPNKSFKVKFGLIQLTDI
                     QEVVLDKLEKELPDFLFYHNVKHTVDVVTEVELIGWAEGCTDEEILLLKTAALFHDAG
                     HTVSYDDHEYHGTLLVKEMLPAYGYSEEQLEKICRIIMSTKLPPKPGDLLEQIICDSD
                     LDYLGRSDFIPVSNTLYEELKAQDKITNLNDWNKMQVKFISGHQYFTATARQLREVNK
                     KLQIERIRSLITES"
     gene            complement(17854..20160)
                     /locus_tag="PLA17_00615"
     CDS             complement(17854..20160)
                     /locus_tag="PLA17_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPK83943.1"
                     /translation="MKRICSLLCLALSSLLVSGQGSVGSWTDHLPYSRSYSISAGGGK
                     IYSSTGSAVLVHDPGSGTNSSLSRVSGLTETAVALVAWCEAEESLIIVYRSTGIDILK
                     NGVITTLPDIKNKYIPGLKEIYGVTVSGSRALLTASFGIVVIDIRGRYVADTWRPGPD
                     GETNSVNEAVILGDRVYAATARGVWSAPVSRQGLSYFGNWELLDDLPEPISEYGMIAS
                     AGTGLMICKPGKNGPASSADSLFLVTPGQPATLIATEPAGTIRALDSDGNRVMVSLSS
                     SLRILSPQGAVTREITGYGWAGASPNGALTTGQDLWVADASVGLVSTTNFTDFRNHAI
                     AGPYTSNVADIVFSGNSFCVTGGTVDNAWGNVYRPLQVFTGSANSWKSHILYGEADRD
                     AMRAAIDPANENHLFVSSWGNGLYEFLDGRLVQNYNQYNSPLSSIIPGKNYSRICGLA
                     WDLPGNLWMTQTGVQGNLKALTPDGNWIITEVNLNMDVAGDMAIDRNQYIWVILPRGN
                     GLLVYDPAGTPSNTSDDRFIRLQVQDADGQIMNNLFSIAVDLDGNIWVGTDMGPAVFY
                     NTGKVFSSEIRASRIKIPRNDGSGLADYLLGTETVTAVAVDGANRKWFGTLSAGAFLM
                     SDDGRKELAHFTTTNSPLLSDNIVRIAVNGVTGDVWFGTSEGIISYRGEATSGKKDFS
                     GIYAFPNPVREDFNGVVTITGLVGNSSVKITDVSGNLVYETKSLGGQAVWDLRNYRSQ
                     RVATGVYLVFCANEDGTLAGVTKMLVIR"
     gene            complement(20172..20768)
                     /locus_tag="PLA17_00620"
     CDS             complement(20172..20768)
                     /locus_tag="PLA17_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HPK83944.1"
                     /translation="MELVFATNNKHKIKEINDLLDDNFTILGLADTGITEDIPEEAET
                     LEENALFKARYVHEKTGLNVFADDTGLEVPSLGGAPGVHSARYAGESKSFDDNIEKLL
                     REMKDMTERSARFRTVIALILDGSEYLFEGTVEGEIIRERRGSGGFGYDPVFLASGYD
                     LTFAEIPLSEKNRVSHRAKAMRKLIGFLSDQKQTGAGK"
     assembly_gap    21213..21312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21313..23118
                     /locus_tag="PLA17_00625"
     CDS             <21313..23118
                     /locus_tag="PLA17_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPK83945.1"
                     /translation="TVMNTALSAGGVSAQSIIRPSGRLTGIYLYQSGHMIDTAETVPV
                     LRYRDGIVFTMEDDALVEYSYFMAGPDSEWTVWTDRAYKEYTNLDAGRYTFKYRYREK
                     GAGDSNGGGSFSFRVRPPWYFSLLALIIYPLLLIAGGIMMRRRTHRLYMERQSRLEQL
                     IAERTEELQHEKEKSDSLLANMLPKGTAEEIMSKGKADKRKYNFVTVLFSDIQGFTKI
                     AEEMNPETLIDELDRFFFHFDSVVEKYRIEKIKTIGDAYMCAGGIPERNRTNPVEVVL
                     AALEMQKYMQEMKSDPSRPAARFWDIRVGIHTGTVVAGVVGHKKITYDIWGDTVNTAS
                     RMESSGEAGKINISGTTYEFVKEFFTCDYRGRMPVKYKGDLDMYFVTGIRPELREPDG
                     SPNRKFLARIEMIRILDVEEHVFSRYSEIASPDLFFHSADYIRSISLQADLLARAENL
                     PDEEYIHLRLASIFIYFGYAFDYNDPEAAALKRAGEILSFYGFGAHTLEAVRELIASA
                     SSPEQTGMAGRVLHDAVYDFTGRVDFISMVDKLYREEKAYGKTGDPQVWFSELASRIG
                     ENRFLTETARRLRAVAEDEQLRALREFAGGRLDNA"
     gene            23176..24192
                     /locus_tag="PLA17_00630"
     CDS             23176..24192
                     /locus_tag="PLA17_00630"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPK83946.1"
                     /translation="MTNLDWNNLPFGYIKTDFNVRCYYKNGQWSKPEVSDSEYINIHI
                     AATGLHYGQEAFEGMKAYRGRDGKVRLFRWDENAKRLISSADGIHMAPVPMELFREAV
                     VKTVKLNEKFVPPYGSGASLYIRPLLYGSGAEVGVKPASEYTFIVFVTPVGPYFKGGV
                     KPVDMLICRDVDRAAPLGTGNIKVGGNYAASMRAIVNAREGGYGNALFLDAREKKYID
                     ECGPANFFGIKSNTYITPKSESILPSITNKSLITIAESMGIKTERRRIPVEELAEFEE
                     TGACGTAAVISPIAKIFDPDTGKVYEYCKDGKPGPVSMKLFNKLVGIQYGDEPDEFGW
                     MTEV"
     gene            24305..25183
                     /locus_tag="PLA17_00635"
     CDS             24305..25183
                     /locus_tag="PLA17_00635"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPK83947.1"
                     /translation="MAKSTGGISRYRLGSSYVTLVVSVSLVLFLLGILGMVLINARQL
                     SDYFRESLSFTIMLKEDAREADIRMLQKELDAKPYVKLTRYVSKDVAAMNLREELGED
                     FLDFLGYNPLMPTIDVYLLAEYTQPDSVIKIEKVISEYPVVDEVYYQESFLNLINDNF
                     RRISVFLLVISFLLFLIALTIINNTIRLSIYSKRFLINTMQLIGANRSFIRKPFLLRS
                     LFFGFLAALIAIGLLMGLMYLVEKEFFTLFTYENINLFLLLCLALVVAGVIINWISTF
                     FAVNRYLGMHEDKLYY"
     gene            25191..25439
                     /locus_tag="PLA17_00640"
     CDS             25191..25439
                     /locus_tag="PLA17_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPK83948.1"
                     /translation="MVAKKEEEKKTGFALGRENYKLMAIGFAVIIVGFILMAGGRSDD
                     PNVFSDKIFSFRRVTLAPLIVLAGFIFEIYAIMKRPRD"
     gene            25473..26249
                     /locus_tag="PLA17_00645"
     CDS             25473..26249
                     /locus_tag="PLA17_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPK83949.1"
                     /translation="MNWYEALIFGLVQGLTEFLPVSSDGHLEIVKFLFGGVEESFLFS
                     VAVHGATVLSILAVFWKEILKLLKGVFKFEMNEETVYTLKLIVSMIPVAIVGFTMKEQ
                     VESLFIADMDITGSFLLVTALFLLIGHFVPKRNKPISYGNAFVIGIAQALATLPGLSR
                     SGSTISTGLMLGNSKDELARFSFLMVIVPILGANFLELVTTETTVVSGVLVPLVIAFV
                     AAFVSGYVACRWMINIVRKGKLGWFALYCILVGLSLIIFA"
     gene            26255..26974
                     /gene="truB"
                     /locus_tag="PLA17_00650"
     CDS             26255..26974
                     /gene="truB"
                     /locus_tag="PLA17_00650"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPK83950.1"
                     /translation="MKRCSDFEDGEVLLFQKPVYWTSFDLVNKVKLAIRHHCGIKKLK
                     VGHAGTLDPLATGLMIICTGKATKRMNEFIDLDKEYIAVIRFGQTTPSFDLESEVNAE
                     YPAGHITEGLIREKLKGFLGEQEQEPPLFSAKFVDGKRAYELARKKIEHKLESVKVVF
                     REMELIDYSGVDATIRIVCSKGTYIRAFARDLGQALGSGAHLRSLVRSAIGDFRLEKA
                     LSVEEFKFFLENLKHNENSYV"
     gene            27002..28051
                     /gene="queA"
                     /locus_tag="PLA17_00655"
     CDS             27002..28051
                     /gene="queA"
                     /locus_tag="PLA17_00655"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPK83951.1"
                     /translation="MKLSQFKYTLPQELIALYPSKNRDESRLMVVHMDTGEIEHKVFK
                     DIVEYFREHDVLVFNNTRVFPARLYGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGEDDMLVAEVIDNTTSRGRTLRFLFDGPYEEFKKTLYSLGKTPLPKFI
                     NRPVEPEDNERYQTIFAKHEGAVAAPTAGLHFSRELLKRLEIIGVEFAEITLHVGLGN
                     FRNVDVEDLTKHKVDSERIIISEEASALINKAKERKNRVCAVGTTVVRTLESSVSTSG
                     MVKPFNGWTNKFIFPPYEFKVPDMLVSNFQLPYSTLLMMVSAFAGYNLLFEAYRTAVK
                     EKYRFGTYGDAMLIL"
     gene            28247..>30291
                     /locus_tag="PLA17_00660"
     CDS             28247..>30291
                     /locus_tag="PLA17_00660"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HPK83952.1"
                     /translation="MLNYKRAIFSLLGGILALLFELSVYGQCGPGKKAMPIQGTGSIV
                     TQNVGVELPENILGNPDGTGAQFHDNGDYIVVDFIDTILAGQSYVITWRQSPSTPGTS
                     RLYWREALVVGTYMAPRSITTTNEQFASVILTANSDTRYLRLYNSNGIDFEVDAVTYT
                     TTKCFSDDCGGNMYLQILSGNALPPGAGDNHGVQNASDIAGVPDGKYGIFDSNGDWVI
                     LNFPYTVPAGQQYSIIWRPLDMTGASSSTMAIQESDGGTWGGLKTLTPYSGESPIFLT
                     EVVTASQNTNRIRIRISPGSDYFHIDAVVFNVAGCQPPPPVLNVSGNLVYCDNAIQIS
                     PGLIIDDPYDQIIPAVYVQISDGFQAGQDVLSCTPDFGITPTYLSAYGLLILKGPATT
                     SQYQSVLRTLTYSNSSPSPVAGNRNIIISFERYNPITGHYYRYLPTPGLSWHEARLNA
                     GRTHLYGMQGYLVTIGSAAENTFLRSQMAGGAVWTGASDSFSAEGDWKWITGPEAGTS
                     FWLGDENGAELTYANWAAGEPNNNDDEDFGHYLSDGTWNDIGYFPGSGIYASEGFYAE
                     FGDMPGDPAPDIFGTVTVNVVTAESLNPVITGPDTVCPNATGQVYSTPNVPGASYLWE
                     VTGGIIAEGQGTNSITVDWGATSPGIVRVTESAGASCQVTTADTVIIADMIKPVITG"
BASE COUNT         7467 a         6834 c         7860 g         7930 t
ORIGIN      
        1 acctcttcgt tgaaaagtat ctttagattt ttcctggctt tcatagcggt aaatttgctt
       61 ctgggttaaa ctgaattacc atgtacgaat ttttccggca ggggcatttc atagactttt
      121 tttctgaggg attcacccag ggaaagaggg tcggggaaac gcgactgttc ttcaacgctg
      181 atcagttcac cgatgccgat tctctgttga ctgccccttt tgttaaacat ctctgacggc
      241 agtctcagca cccgcaccct ccagtttatc agtccgagga agtaaaagaa agccgagttc
      301 atgtcaaaaa acctgattgg cagcaccggc accttcaccg agtggatcag gtggagtatg
      361 ctttcctgcc atggtcggtc ccttactttc atgtctctca ggctgaagtc tgagacagca
      421 cccgaaggga agaatccgag aggatgtccg gcgccaagat gttcgatggc ttccctgatg
      481 ccctttatgc tggttgcctt gatagttgtc tgtttgtcac cttttggcac aacggggata
      541 aagttttctt cgagggtttt tatcagggag aggagttcgt tgacaatgaa tttgtaatcg
      601 ggcctgatcc tggccatcat gtcaatggtt ataattccgt cgagccctcc gtaaggatgg
      661 tttgagatgg ttataaatgc gcctgccggc agatctccaa gtctttcggg gttaccaacc
      721 acgtagctga cacctatatc tttaagcagt cttgtggtga attcggaccc gctccatgct
      781 cctaatttgt catatgccct gttaaccttg tcaatagcca gtgacctgat caaccactcc
      841 gcaaagcgct gccctttagc gcctctgaac aggaatgaca acggttcgag atcttttggc
      901 tcaattactt tcatggcgta aattccaaat ttactaaata gtcgactctt aagaagtcat
      961 gtttttcaat aattctgtac agtggcaatt tttacagacc agttgccggt caataaactc
     1021 aggacaaagt aaattgtaac acggatctgc ctttatcttt tcatcatatt aaaggcaacg
     1081 actgccataa tagtatttat catatcacct tcgactccct taagatagtt ccctggcatc
     1141 cggtaatcac tcttgagatg tctgataaca ggttcaactg atgcctgatg tatgaaccgg
     1201 gccctggcct tatgcttctc ataggctgtt ttgcatctca ttttgcaagc tttttaacga
     1261 atctagcaaa aagcctgcaa gctgataaat attaacatta acttatatca ttagatatca
     1321 gtaatataga tgctttatac aggttgacta attaataacc atcgaaccat gcaggtttaa
     1381 tatctgccat attgcggatg atgagaaatg aaatgaaatt cgtatttcaa aaaccatata
     1441 tttggaacgg agttttccgc agtatttgaa acagtcgtaa aacgtcagac tatgaaaaag
     1501 ctttgcctgg tcatgcttac aattggcctg ttctcctgtc agagtaaccc gacaacaggt
     1561 ccggcagatc tctcaaggaa cactattatt cccaaaccag tgtcggtgac ttccgccgac
     1621 gggacattca ttctcacctc caggagtgac atctttgtgg ctgagggcag tccggaagag
     1681 gtagccgtga tcggcaatta ccttgccgaa cgacttaatc catcaaccgg cctcggactc
     1741 agggtgatca ctgacggtcg caaacccggg aagggagaca tacttcttgc cataaacgaa
     1801 ggtgatacgg agctgggcga ggagggctat gagcttgtca ttaccgggaa ggttatgacc
     1861 ctgactgctg tcaggcctgc ggggctgttc cggggcatcc agaccatcag gcagctgctt
     1921 ccgcccgcga ttgagtctgc caccattcag ccggggccct gggtcatacc cgcgggtacc
     1981 atacgtgact ggcctgaata cgggcatcgc ggggagatgc ttgacgtggc acgccatttc
     2041 tttggcccgg aggatgtaaa gaggtttatt gatcacctcg cactctataa gatgaacgta
     2101 tttcacctgc atctttctga tgaccaggga tggcgcatag agatcaaatc atggcccaac
     2161 ctgaccaccc acggcgggaa gaccgaagta ggcgggggcg aaggaggctt tttcacccag
     2221 gaagagtatt cagacattgt gaaatatgct gaggagaggt acataaccat aatacctgag
     2281 atagacatgc ctggtcatac gaacgcggcc ctctcatcct accctgagct caacagtgac
     2341 ggcaaggcaa aggagccata tacgggaacg aaagtcggat tcagctcact tgacacccgc
     2401 tcggagatca cctacaagtt tgtggatgat gttgtccgcg agatcgcggc tcttacaccg
     2461 gggcagtact ttcacatagg tggtgatgag tcacattcaa ccaaaattga tgattacatc
     2521 tactttgtaa acagggttca ggatattgtt gagtcacatg gcaagagtgt taccgggtgg
     2581 gacgagattg cccatgcaac gcttaagccg gatgttgtgg ttcaatactg ggcgcggccg
     2641 gcaaacgcgg tgaagggcat tgcacagggt gccaaagtgc tcatgtcacc ctgccagcat
     2701 gcctatactg acatgaaata tgactcgacc acggttctgg gactgagctg ggcggcatac
     2761 atagaggtgg atgatgccta caactgggac cctgccacac ttgtaccgga gattaagagg
     2821 gaaaacatag tcggggtaca ggcgccgctg tgggcagaga ccatctcaac atttgatgaa
     2881 gctgaatatc tgatcttccc gcgccttccg ggatatgccg agataggctg gactcccgcg
     2941 gaaatgcggt catgggatga ataccgggtt cgcctggctg cacatggcaa aaggatggag
     3001 acactcggaa tcggatttta ccgctcccct ctgatcgatt gggatcagcc gggcctatag
     3061 aaacgtatat accgggttaa tactgattat aaacaattca ctataagatt atggaattct
     3121 tacttgacgg tatcaccaat gttacatggc aacaggctgt gatgtgggtt gtcggggctc
     3181 tgctgattta ccttgctatt tcgaaagaac ttgaaccgac gctgctgttg ccaatgggtt
     3241 tcggtgccat ccttgtcaac cttccgttgt caggggcact gaaccagacc ctctcgggga
     3301 taggagaggt gacaggtatc ctggactggt tgttcaaggt aggcattgaa gcgtcagaag
     3361 cgatgccact gttgttgttc attggaatag gggccatgat tgactttggt cctctgctct
     3421 ccaatccgag actactgctc ttcggcgcgg cagcgcagtt tgggattttc gtaaccatac
     3481 tcgcatccgt tctgctgggt tttgagctca gggatgctgc ctctatcgga attatcggag
     3541 cagcggatgg ccctacatcc atcctggtat cacaggtgct taagagtgat tatatcggac
     3601 cgattgccat tgcggcatat tcctatatgg cactggttcc cataatacag ccttttgcca
     3661 tcaagatgtg taccactgag aaggagcgaa agatcaggat gagttataat ccgaagagtg
     3721 tttcacggat gacaaggata ctgttcccga tagcggtcac tattattgca ggtttggtgg
     3781 ccccggcatc gattgctctt gtgggattcc ttatgttcgg caacctgata agggagtgcg
     3841 gtgtgctcag gtcactgtct gaaagtgctc agaaggagct ggtgaacctc ataaccctgc
     3901 tgcttggaat aacaattgca tcaaccatgc aggcggaaaa atttgtggcg ctcaacaccg
     3961 tcaagatcat ggggctgggc ctgatggcat ttgtcttcga tacagttgcc ggagcctcat
     4021 ttgccaagct tatgaatgtc ttcctcaagg aaaaggtaaa tcctatggtt ggcgctgcag
     4081 gtatttcagc attccccatg tcttcaagag tcatacagag actgggcctg gaggctgatc
     4141 ctacaaacca cctgctgatg cacgcagcag gtgccaatgt ttccggccag attgccagcg
     4201 tcattgccgg cggtgtgata ataggccttg tacccggatt attgtaaagg aggtaaatca
     4261 tgaatccaga tataattaag tcgcttgatc tgatggggaa gggcatgaca gccatcttcg
     4321 tcgtcattat tgtaatctac ctttctgtta accttatgct gaggcttaca gaaagcaaga
     4381 agaaggagac gaaagaatag agttccacag atacttttcg gagcgtctgt gactacctgt
     4441 cttaacgcct gtaattaata gaggcggatt ggtcgtaaat tttagtaatt ttatatggcc
     4501 gttttccccc tgagggggta tgcggtatat gtcggaccaa tcagtgaccg gatgaagaaa
     4561 gcgcttttat tgattctcgt tgccatcatg gcatcatgca gccagatgag aaaaggttct
     4621 tccgcaacgc cgaaagatga gctggccatt accaggaaat atgttggcga ttttattgag
     4681 taccgtcata cggggccgga gaactatgac ggcccgaata tgatctggat cagaaccacc
     4741 atggaggacc agtacggtat aatatcggcc tacgggcgca gatgtgactt caccagtggt
     4801 gacaggctct atctgagaaa aaaattttac agcccgggta ttattgcagg ctattgggtc
     4861 tatgtgattg aaaatgatga gtcggtatca taccgggcca ctgatctcca gcatgaccgt
     4921 gaggtgtaca tcaaatcatg gtttgaatga tttgatcagc agccgttgca ggtgaatacc
     4981 gatggccact gacaatgcct tttccccggc tatatcaaaa ctgccgggca acatggtgcc
     5041 tgccggcacg aagctgaagc cggccagcct gccgcaggga aataaacaga cttaaatcag
     5101 ttgaaccgca tgatacagag agacaatacc ctgtacttca ttgctctcat tcccgggaag
     5161 gagctacggg gaagggtgcg cgccctcaag gagagaatga aggctgacta caacgccggt
     5221 catgcactca aatcaccagc tcatattaca ctgcagatgc ctttcaaaag gaacagcagg
     5281 gatgaagatt ccatctctgc cctccttgaa aaattttcct ctgaagaaag gccttttacc
     5341 gtcaggcttg atggatttgg ctgcttcgca ccgagggtta tcttcatcag ggtaagggag
     5401 catgagcctg taaaagcatt gcacagccgg ttaaaggtta aactgagtga ggagcttggt
     5461 ttttcagaaa gtgagatcat gaatgacgtc cagccacaca taactgtcgc cacgcgtgat
     5521 cttacgaaag aagcattcag ggaagcgtgg cctgaatttc agaatgagga attcaggggt
     5581 acttttgagg ttcacagtct ctttctgctg aagcataacg gcaagagctg ggatatactt
     5641 aaagaatttc cgttcagaaa aaatggatag aaggaggttt atctcgggaa gcgcccttgc
     5701 tgctgccgga tggctggcgg cgtcacgtct cagggcagtg ccatggtcac ctgacggtgg
     5761 ccgggttccg atgccggcat cttccatccc ggggtccgtg actcttgccg gcaacgggct
     5821 gtgggagtca ttccgtgatc cggcaggaca ctaccatccc ttcgtcaggt ggtggtggaa
     5881 cgggaacaag attgaggcag gtgagctcat cagggaactg caccttctca aggctgcagg
     5941 aataggaggt gttgagataa acccggtggc attcccatcc cgttttgacg gtgacgatct
     6001 tggaaagcct tcactggagt ggccgggtga agagtggtgc aatatgctga atatcgtgtt
     6061 cgccgaggcg aaacggcttg atatgacctg cgacctgatc gtcggttccg gatggccttt
     6121 cggtgcagag aatactgctg aagaggagag gtcacaggta gttgtcgtcg ccgtccggaa
     6181 ggtcagcggt ccttcagagt taaatatatc acgggaagag atcttcatgg aggccggccc
     6241 ggccatcaat aatccgtggc ccggtaaaaa gtattccctg ctttccatga tgctgacgcc
     6301 tgatccgctt acttcagcag aggagataac agacctttca gacaggattg gtgaggagtt
     6361 ctttgacctc ttcatacctg aggggaatca tgcgctctat gcgctggtaa aggtcaccgg
     6421 tttcatgcag gtgatcaacg gagcaccggg tgcaagcggt ccggttattg accactttaa
     6481 cagcgatgcc gtcaacaggt tcctcaacag gatgtcagac tccattgaac ctgtgaccgg
     6541 gcctctttct gaccatcttc gtgcactctt cactgacagc atggagcttg agggctccaa
     6601 ctggacaggt gacatgaggg agcagttttt caaaaggagg ggctatgata ttttcccctt
     6661 cctgccattt gtactgttca ggacgggctc catgggcaat gttacagatt tcaggtatgg
     6721 agtggagtac ggacctgctt tcctggagat gatacgccgc atgcggtttg atttcgagac
     6781 aacgaaggcg gagcttcttg aggagaggtt catacagagc tttaccggat ggtgcagaga
     6841 cctgggggtc agatcgcgca tgcaggctta cgggagaggc tttttcccgc tggagagcag
     6901 catgcccatt gacatcccgg aaggagagtc gtggaccatg aactggctga agcacaggat
     6961 aggggaggag atgccggagc acgactaccg tcgcggcagg gcctacacaa tggtcaacaa
     7021 gtacgtttca tcagccgccc acctgaccgg taaaagggtg atcagctgcg aggagatgac
     7081 cgacacttat actgtcttca acacctctct tgaaaacctt aaggttggca gtgaccagag
     7141 cattatttcg ggtgtgacac attcggtatt ccacggcttc aattactcac cgcctgaggc
     7201 tccctggccg ggatggataa ggtacggatg ctattacaat gagaacaaca actggtggcc
     7261 tcattttcat ctgctgaatc agtacaaggc caggctgtcg gcgctgctgc agaactgtga
     7321 catgtatgct gatattgcgc tgttgccgcc catggctgac atgtggagcc tcatcgggat
     7381 gcagaatgag ccgttcccgt cggtgctgca cgccgaacac ctttcactgg tatgggaggc
     7441 gatagccaaa aacggcagcg gctgcgatta tatctcggaa agaatactcc gtgatgccac
     7501 ggttgaaaat aaaaagctct gctacggtcc gcggaagtac aattcactgt tcctggtcag
     7561 cgtggagagc ctctctcctg atagtgcacg caaactgctg gagttcgtga gagcgggagg
     7621 cagagtattc tgcgtggaaa cggtgccttc aaaatcgacc gggtggagca accatgaaag
     7681 aaatgatggt gaggtggggc tcattgttga aaagatgaaa gcctatccgc accggttcat
     7741 ctgtcttgat aagcctgaaa aagacattat cggatggtac aggagtgttc aggagcggta
     7801 ccggatcaat ccctggatca gaatagataa acccgatact ttccttatgc aggtaaggta
     7861 cagtgctgat gacggctccg ggtacatttt catagtcaat tcgcatcttc atgaatcacg
     7921 caccacacgg gtccgtttct ccgccgggct gacacgcagg cgccactgtt ccgtatggaa
     7981 cccggagaca ggcgggagaa aacgggtaaa acctgatccg gatggcagcc tcctgctcga
     8041 tctcggccct gctgagtcac ttctttttgc ctttgacagg gaggaggcag aggaggagtg
     8101 gaggccgctg ccctctaccg gaagtgacat gcaccttctt gataaaggct ggagcgccga
     8161 attcaggcac tgccgcgacg gctcggtaaa ggaggtgatg atggaccggc tgacagattt
     8221 gaaagagatg cctgagtttg tttacttttc agggagggtg atttacagga acaggcttga
     8281 atgcactgat acggcaggga tggtgttgaa cctgggtaaa gtctacggaa cgtctgagct
     8341 caggatcaac ggagtaagct gtgccgtcaa atggtacggc cggaggatat tcagcataga
     8401 agaatatctg aagccgggga tgaacacggt ggaggtggag gttacaacct caatgggcaa
     8461 ttacatgaaa tcactgacag acaatcctgt tgcacagtac tggacgaatg ccgggacaaa
     8521 agaccagccc ctgcagtcga tgggtataac cggacctgtc agttgctaca gaagatgaga
     8581 tgatatgaaa cccgcatctg tcagatacac aaacacatat tcatataatt ttgagtcacg
     8641 cgatttccac aatgccttaa aaacaaaaat tatatcatga taaaaaaggg tacaatctta
     8701 ttgctggcgg cactggtggc cgtttcgttt ccgcttcatt cgaaggacta caacgcatcg
     8761 atgtttggcg ctaagtcaaa cgggaccaca ctcaacacca gctctatcca gaagggtatt
     8821 gactacatca gtgagaatgg cggcggccgc ctggtattct acgtggggcg gtacctgaca
     8881 ggatcgattt atctcaagtc aaatgtgacc atccaccttg aggagggagc catcctcgtg
     8941 ggatctgtga acccctttga ttatgacagg aatttcaact ggaccgccct gatctttgcg
     9001 cttgaccagg aaaacatcgg aataaccggc aagggggtta ttgacggcca gggattccag
     9061 gtagccacca accttgttga catgatccac aagggggtgg taaaggatcc tctgaaatat
     9121 gaccgtccaa atgagaccat acgcccgcag aatatttatt tcaggggttg ccggaacatt
     9181 gttgtcaggg acatcaacct cagggatccg ggcagctgga accagcaata cgaccagtgc
     9241 cgcaacctgg tgatagacaa tattaccgtt gacagcaaga gttactggaa caatgacgga
     9301 gtagatatcg tggattgcga cagcgtggtg gtctccaatt catactttga tgctgcggat
     9361 gacggcatct gcctgaaatc ccacagtccg gactttgtgt gtcagaatgt gtacatccat
     9421 aacaatacgg tacgaaccag cgccaacgga atcaagttcg gcacggttgg ccatggcggt
     9481 ttccgaaacg tcaggataat caacaacgtg gtatttgaca cctacaggtc agcaatcact
     9541 tttgctgcag tagatggcgg ttttgtggaa aacattgtgg ttgacagcct ccgttcactc
     9601 aacaccggca acgtcatttt cctgaggatt ggtgaacggc gtgccggtaa aaagggaagg
     9661 atgaacggtg tgtcaatatc caatgtctat gccgaggttc cggctacaaa gcccgatgcc
     9721 ggctacaatt acgagggtcc ggtggaggat cttccgagaa acatctctcc ttcatcaata
     9781 gttggcatgc ctgatgccat gatagagaac attgccctga agaatatcga gatacattat
     9841 cccggcggag gcaatccaca ttacgcgaaa gtgggtcttg atgagcttga caaggtgcct
     9901 gagctgggag caaactaccc ggagttctca atgttcaagg agttgcctgc atggggtttc
     9961 tacataaggc atgccagggg aatcaccatg gagaatgtga agctggtatg cggaaagaag
    10021 gattaccgga ccgctgtggt gttagatgat gtacacgggg caacgttcag taaactgcag
    10081 gtcactgaac ccgggagcag gaaggagagc atcttttccc acaattcaac tgagatcgta
    10141 aacagaaaat aggaaactct gtctgaattc atttcgaaga atgccggaca acccggttga
    10201 cctgaggagt gccggcatag ccacggtgaa aaaacagagc cagctgtcct catagcgggc
    10261 acaagtatta cctgatatgg aggactataa aaagtcacac ggccctgaga tgggccgtgt
    10321 ggctggtgac tttatctctg gtcctgagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnntg attttatttc ttctccggaa ggaataccag ctcgaggtgg tctgctttgg
    10501 agaggaattt ccttgtgacc tttttcattt tcttaacggt aagtttgttg agaatatctg
    10561 caaagttgcg gctgtcatta atgtcaaacc cgttaaggta gtatgaactg actgcgtttg
    10621 cccagtagct gttgtgttgt atagcctcct cgcgggtctt gagcaggttg cttacagttt
    10681 tatcaaggtc aacctgtctc gggccgtttt tggcaatcgc ctcaagttcc gaatagatta
    10741 tattcttgag ttcggaagct ctttccggat cgcattcaaa tgctatcagc agtgttgctt
    10801 tttctaccgg tcttttctga gacccggcat taaggctgac gccgtaggtg cctccctctt
    10861 cttccctgac tttttcagta taaaccaggt caagtatgcc tttcagcaca tccatggaaa
    10921 ggtaaccgga tggattgtat gcagcatctg ccgaatagtt tataacgatg gttgcctttg
    10981 gcacggtaag cgggatgaaa atatcctttt tgactgttcc ttttggctga tccataccca
    11041 ggttccggta tgtctcagac tggtattttg aggggagtga tccgatatag atccgggcca
    11101 tttcacgggc tacagcttca tcgatgttac cggtgatgaa gaatgtaaat gccgaagcat
    11161 cgtcaaaagc cgtcctgtag atatagttta tgtcatcata tttaatacgt ttcattgatt
    11221 ccggagtcag caggaatgtc ctcgggtggt agttggtcat gttcattgag aggctgtcag
    11281 acaagatctt gtcgggattt ttctgcatca tggtgattac tgccgtgaat cttccgatga
    11341 tagcgtcata tgcctccctg ttgaagttgg ggcgggcgaa gcgaaggtag agcaattgca
    11401 tcattgtctc gaaatccttc ggtgtggaag agccgctcac tgtctgtctg gtctcctgga
    11461 tgccgagtga tacggaggcc ttcttaccgg ccagcatctt tgtaagggcc acattgtcaa
    11521 attctccggc tccgtacatt gagatcacct gcgggaagag gttcagggca ggttcaagtg
    11581 aatcggggta gaatgatgat ccgccgaaag catatccgct gatggtaaca ttatcctttt
    11641 cataatcggc atgcctgaac accaccctgg ctccgttgga gagggtccat tcggtggctg
    11701 caaactgtgg cagggagacg gttttgacta tttctgctcc ttccagttct tcagatatga
    11761 ggtctgttcc tcctgtgatg tcttcatagg gttcgatctc tgctgccgcc accttttcca
    11821 ttatggcaac agccccggcc tctgtcatgt actccctgtc atcgggcccc tgtacataaa
    11881 tgaacctgtt gtcatccttt acaagctcct gaagcctgga gttcacctct ttcactgtta
    11941 tcccgggcag aatattcttg tagtaatcat actggagatc gagcgaagga atgggttctc
    12001 cggtgaggaa gtgatttttg atcattgaag cccatgaatc attgctgatc ttgtctttct
    12061 gcttatagcg attttcgaaa gtggagagca tctcagcctt agccctgttc agttcccctt
    12121 ctgtaaatcc gtgccggcgt gcgcgttcca tttccgtaca ggcaacttca aatcctttgg
    12181 cctcctctcc ttcattggca taaacagaga gtgtgaggga attgtagttt ctcgccaggt
    12241 ctgaagagta acgcagtccg ccagagacaa acggaggagt tcctttctgc actatctcac
    12301 tgaaacgctt gctcatcatc atgttcataa gcgatgttac atatccgtca cggatgtagt
    12361 caagattcct ttccctcttg tcaggttcgc ggtcaattat cattaacccg acttctgtcc
    12421 ccggggcctc aggatcagta acaaggaggt actttgttcc cttctttgcc gtgaggaggt
    12481 tttcaggcct tgggattggg ttctctattg ccggaatggg agagaagatt cttttgattt
    12541 ttccttcaac atcatcaacg tcaaagtcgc ccactacagc tattgcctgc aaatcagtac
    12601 gataccagtc gtggtaaaag gctctcagcg cttccggatc aaagttttta atcacggcag
    12661 tgtcgccgat gatatccctt ttggcataca ttgatccttc atagatcact ggcagcatct
    12721 gtgtcagcat tctccactgt gcatttcgcc tgcttcgcca ctcttccagg atgacacccc
    12781 gctccttgtc tatctcctct tcgtcaagag tcagaaagtc agaccagtcg gcaagaatca
    12841 tgaggcatgt atctatcagg cccggtttat caacaggtac atcactgatg ttatagactg
    12901 tttcgtcgaa gctggtgtag gcattgatgt ttcggccgaa ggccactcca tgtttctcca
    12961 gggtgctcag cagggttttg cccgggaagt gagctgtgcc gttgaatgcc atgtgttcca
    13021 ggaaatgggc aaggccgttc tgatcatcct gctccaggat ggcccccacg ttctggatga
    13081 tgtagtaact ggccctgcct gccggttccg tattgtgacg gatgtagtag gtaagcccgt
    13141 tctcaagctt tccgatacgg taagccggat cagccggaac gtttttgtcc ggtgatgcct
    13201 gtgcagcgat aagatggctg acagcagcca aaacggcaat tgctagaatt cttttcataa
    13261 tctttatatt gttggactta ttagacaatg acatcacttg aagtatcaaa attaaataaa
    13321 aaaaaccatc gcgccgatgg ttttttagat ttctttaagg caatgtttaa aacagcccgt
    13381 gcagctcggc attgatcctg tcaatgacct cactgaggtg ttcattgttt tcagggaagt
    13441 cataatagtc agcttcgagt ataaggagtc tgcccagttt gtaggattcg atccatgcct
    13501 cataccgctc attcagtctt ttcaggtaat cgagcctgat tgagctttcg tactcgcgtc
    13561 cccgcttttg gatctggttg accagtgttg gaacggatgc cctcaggtag agcaggaggt
    13621 cagggggctc aacgagtgag ctcatcagtt tgaagagggt gaagtaattc tcgaagtcac
    13681 gtgttgacat gagacccatg gagtgcaggt tgggggcgaa gatatatgcg tcttcgtaaa
    13741 tggtgcggtc ctgcactatg gtttgttctg cctttttgat ttcgagaatc tggctgaagc
    13801 gggagttcag gaagtatatc tggaggttga atgaccatct ctgcatgtct tcatagaagt
    13861 cattcaggta aggattgtca tcgacatcct catagtgagc ctgccagccg taatgtttcg
    13921 cgagcagtcc ggccaatgtt gtcttgcctg atccgatgtt gcctgctatg gctatatgca
    13981 tatgaaatga ctgttaaaat acgtgcttaa atatatgaaa aagggaggga gtagtcctcc
    14041 actgttgata aaaagatttc agaggccggc tgttttcaga acgctctcaa cgatctccct
    14101 gttgtttgac aaccgcggca tcttgttctg cccgccgagt ttgtccctct ccctgaacca
    14161 tttgtagaat gttcctctgg gaacagctat catcctggga cgggcaagtg tcaggtcttt
    14221 ataccttttt gcctcataat cagaattaag ggctttaagg gtagtgtcaa gtatttcaat
    14281 gaaatgctcg gggtcatcag gttccctgtc aaattcgatc acccattcat gtgctccgcg
    14341 gctttcgtca cccatgtaga ccggccctgc cgtatattcg cttatgtgcg catgcgtgcc
    14401 ggagcatgcc ttttcgaggg ccttttcggc attgtcaatt atcagctctt cgccgaaggc
    14461 attaatgaag aattttgtcc gtccggtgat tttcatcttg tgaggataaa ggctggtgaa
    14521 ctcaacagtg tcacctatgg catatctcca caggccgccg tctgtcgaga ttaccattgc
    14581 gtagtttacc cccctttcaa cctcaccgat gcttagtgtg cgcggattat ccgtgccgaa
    14641 ttcatctgcg gggatgaact catagtaaac accgtaatca agcatgagca gcatcgagct
    14701 gtcagtcaga tcatcctgta tggcgaaaaa tccctcggaa gcgttatagg tctccatata
    14761 gtgcatcttc gggtcaggca ggagctttct gtactgctcg cgatagggtg cgaaactgac
    14821 gccgccatgg aagaagactt caaggttggg ccatacctcg tgcaggttcg ccttgcccgt
    14881 ggtttcaagt atgtgccgga tcaggacgag ataccaggat ggaacaccgg agaggctggt
    14941 aatattcata tcaacagtct tctcggttat catcctcatt ttttcctcga agtcctcgat
    15001 aagggctatc cgtgcaggtg gcgttcgtat cagctcagcg tatgcagggg cattttcaat
    15061 cagtattgca gagagatcac cgaagagtga gttgttgctg aagctgttta tctggtgact
    15121 gcctccgaga gtgagtccct tgccaaggaa gattctggtt tcaggattga gagaggtgta
    15181 cagaacgata caatcctttg ctccccggta atgggtctct tcaagcgatt catgcgttat
    15241 ggggatgaac ttgcttttgg tgctggtggt tccggatgac tttgcaaacc actttacctc
    15301 gccgggccag agaacattct tttcgccggc cctgagcctt tcgatccacg gaatcacatc
    15361 ctcgtatgac tgtacggcca cgttcctgca gaattgttca tgggtggtca ctgaggagta
    15421 cccgtgttcc ctgccccatt cggtgtctct tgacagtgaa accagcctgg tcagtacctc
    15481 ctgctgggtt tcggcagggt atttcctgaa cagctcaatc tgtgagaggc gtttggtatt
    15541 gatccattta atgacagagg gtatgaatgg catcgggtca gttttattca aagatagcta
    15601 aagtgtgact tttttatctg aaaatatctc tcagcacctg gagtgaacgc atctccctga
    15661 ttcccgggag gtggagcctg taatacttca acatcccggc cagaagctct gtgcgttctt
    15721 cgccggacaa tttcagcgag gccagctcct caacaggcat ctgcagcaga aggttcagca
    15781 cccgggctcc ggcaggattc aggtagtcag tgtgcattgg ccgcaacggg gtgaactgcc
    15841 cggtgagcat gtcaaagtaa caggcggtca tgtcagttcc tgtcgggcct attcccgtga
    15901 agtcagtgaa cgcaacgaga aaccagagat ggaagttact gattcctgat gtcatctcat
    15961 caaggctgat taccgatgac tcaatgaaat caaagagcag tttgtttaca tcttcttccc
    16021 tgattacatt gtaaagcagc tcgcttatga acatggcaat ggtggtccgg tggatatcgc
    16081 cgtgaatcct ttttgggata taggccagcg tcatctcctt caggttatgc aactccctgt
    16141 tttcgtagta gtaaagttcc gtattgaaga tattcagagg ctggaagtag ttgaaccttg
    16201 cgtttccccg ctttgatgat attcccttta ccattaccga aaggcgcccg tattcctgag
    16261 tatagaaacg ggctatcagc gaattgtctg aatatctcac atggtggagc agtatggctt
    16321 ttgtcctggt cagcatggcc tgaaagatca cctcaaaagt attaaagttt cataacagca
    16381 tactgcaaac aaagagtctg agtttattag attagaataa ttgtttcata tcaaccagga
    16441 tggaatcaga atccaaagaa cggcttctca ggcgaattgc aaggcttgtc cgccagaaca
    16501 aggagctgga ggagcaggtt aaaaaacttg agcgtgagaa ggagaggctt cttgaaacca
    16561 ctgagaaata caggcttctt gttgaaaaca atgagctgaa aggggtcagg gtggaagaag
    16621 aagataagag tctcaagttc aacatggtca cggtcctttt tgccagcatt cagggtttcc
    16681 cgaacatgac ggaggagatt gacccggcat tgatgatgga tgaactggat gaaattttct
    16741 tcgagtttga ccggatcatc aggaaatatc acgttgaaaa gatcagaacc attggcgaca
    16801 cctacatgtg cgccggcgga attcctgcaa agaatatcac caatccggtg gaagtggtga
    16861 tggcggcggt ggagctgaga cagtttctcc gcgattttga agccgccagg aggggtgaaa
    16921 aacgtatctg gaacctgaag atcgggattc acacggggcc tgtctctgcc accgtttcag
    16981 gcaagagcag ggtctcctat gatatcaagg gcagggcggt gaatacggca agccggattc
    17041 agggcgtatc tgaaagtgat tcaatcctga tctcggtaat gacatatgag cttatcaagg
    17101 agttcttcaa ttgcgattat tacggcaagc ttcccgtaaa atacatgggt aatatccaga
    17161 tgtatcaggt ggcaggtctg ctgcccgaac tggctgctga cagcaacgga gcggttccca
    17221 acaaatcatt caaagttaag ttcggcctca ttcagctaac tgatatccag gaggttgtgc
    17281 ttgacaagct ggaaaaggaa ctgcctgact tcctgtttta tcacaatgtc aaacacaccg
    17341 ttgacgtggt caccgaggta gaactcatcg gttgggctga ggggtgtact gacgaggaga
    17401 tactgcttct gaagacggcg gcgctgttcc atgacgcagg tcatacggta tcttatgacg
    17461 atcacgagta tcatggcacc ctgctggtga aagagatgtt gccggcgtac gggtacagtg
    17521 aggagcagct ggaaaagata tgcaggatca taatgtcaac caagcttccg ccgaagcccg
    17581 gtgatctcct cgaacaaata atatgtgact cagaccttga ttacctgggc cgaagtgatt
    17641 ttatcccggt gtccaataca ctttacgagg agctcaaggc gcaggacaag atcaccaatc
    17701 tgaatgactg gaacaagatg caggtgaagt tcatctccgg gcaccagtac tttacagcta
    17761 cggcccgtca actgagggag gtgaataaaa aactccagat tgagagaatc aggagtttaa
    17821 ttacagaaag ctgatcatcc tttggctgca gggctatctt atcaccagca tttttgtcac
    17881 tccggccagg gttccgtctt cgtttgcgca aaacacgagg tagactcccg ttgccactct
    17941 ctgtgaccgg tagtttcgca gatcccatac tgcctgcccg ccaagggatt ttgtctcata
    18001 gaccaggttg ccgctcacat ctgttatctt caccgatgag tttccgacga ggcctgttat
    18061 tgtgaccacg ccattgaaat cttcccttac cgggttgggg aaggcatata taccggagaa
    18121 atcttttttt cctgatgttg cttcgccgcg gtacgagata atgccttcgg atgtgccgaa
    18181 ccacacatca ccggtgactc cgttaacagc tattcttacg atattgtcag aaaggagggg
    18241 gctgttggtt gtggtgaagt gagccagctc cttcctgcca tcgtcagaca taagaaacgc
    18301 gccggcactg agcgtcccga accattttct gttggcgccg tcaacggcaa cagctgtcac
    18361 cgtttcggta ccgagcagat aatcagcaag tcctgatccg tcgtttcttg gtatcttgat
    18421 gcgggaggct cttatttcag agctgaaaac cttgcccgta ttatagaata ctgccggtcc
    18481 catgtctgtt cccacccata tgtttccgtc aaggtcaacg gcgatcgaaa agaggttgtt
    18541 catgatttgc ccgtcggcat cctgaacctg cagcctgata aacctgtcat ctgaagtatt
    18601 tgagggagtt cctgccgggt catagacaag aagtccgtta ccgcgcggca gtatcaccca
    18661 tatgtattga ttccggtcaa tagccatatc tccagccaca tccatgttaa ggttcacctc
    18721 cgtgataatc cagttgccgt caggggtgag tgccttcagg ttgccctgca ctcctgtctg
    18781 ggtcatccag aggttgcccg gcaggtccca ggcaaggccg cagatgcgtg agtagttctt
    18841 tccgggtatg atgcttgaca gcggggagtt gtactggttg tagttttgaa ccagcctgcc
    18901 atcgaggaat tcatagaggc cgttgcccca tgatgaaacg aagaggtgat tttcattggc
    18961 gggatcaatt gcggcccgca ttgcatctct gtcagcttcg ccgtagagta tatggctttt
    19021 ccaggaattg gccgagccgg taaatacctg aagcggcctg tagacgttgc cccacgcgtt
    19081 gtcaaccgtt cctccggtga cacagaagct gttacctgaa aagacaatgt ctgcaacgtt
    19141 ggatgtatac ggaccggcga tagcgtggtt cctgaaatct gtaaagttgg tggttgatac
    19201 caggcccacg gaagcgtcag ccacccacag atcctggcct gtagtcagtg cgccgttcgg
    19261 agaggctccg gcccatccat atcctgttat ctcccgggtg acagcaccct gcggagagag
    19321 aattctcagg gaagaggaga gtgaaaccat cacacggttg ccgtcgctgt caagagccct
    19381 gattgttccg gcaggctcgg tggcaatcag ggtggcaggc tggcccggcg tgacaagaaa
    19441 tagagaatca gccgaagagg caggtccgtt tttgcccggt ttgcatatca tcaggccggt
    19501 gcctgcagaa gctatcatgc cgtattctga tattggttca ggaaggtcat caagcaattc
    19561 ccagttaccg aaataggaga gaccctgccg gctcaccggt gcagaccata ctcccctggc
    19621 ggtggcggca taaacccggt cacccagtat gacagcttcg tttacgctgt ttgtttcccc
    19681 gtcaggcccg ggtctccagg tgtcggcaac atacctgcca cggatgtcaa tcacaacaat
    19741 tccgaagctg gccgtcagca gggccctgct tccgctgaca gtcactccgt aaatctcctt
    19801 cagacccggt atgtacttgt ttttaatgtc aggcagagtg gtgatgaccc cgtttttcag
    19861 gatgtctatc cccgtgctgc ggtagacgat gataagcgac tcttccgctt cacaccatgc
    19921 aaccagtgcg acagctgtct ctgtcagtcc tgataccctt gaaagtgatg agttagtgcc
    19981 gctgcccgga tcatggacga gcaccgcgga gccggtagat gaatatattt tcccgccgcc
    20041 ggcggatatt gaatatgatc tgctataagg gaggtgatca gtccatgatc ctacgctccc
    20101 ctgaccggat accaggagag aggataatgc aagacagagg agagagcaga ttcgtttcat
    20161 tttatggcct ttcattttcc ggcgccggtc tgtttctgat cactgaggaa gccgataagc
    20221 ttcctcatgg ctttggccct gtgcgagacc ctgttttttt cgctcagggg tatctcggca
    20281 aaagtgaggt catatccaga ggctaaaaag acggggtcat aaccaaaccc gccgctgcct
    20341 cttctttccc tgatgatttc accttcaacc gtaccctcga agagatactc cgagccatca
    20401 agaatcagtg cgattacagt tctgaaccgg gcgcttcttt ctgtcatatc cttcatctcg
    20461 cggaggagtt tttcgatgtt gtcatcaaag cttttgcttt cgcctgcata ccttgccgag
    20521 tgcacaccgg gtgcaccgcc cagggatggt acttcaagtc ctgtgtcatc tgcgaagacg
    20581 ttcagccctg tcttttcatg tacatacctt gccttgaaga gggcattctc ctcaagtgtt
    20641 tcggcttcct cgggaatatc ttcagtgata cccgtgtcag caagtccgag aatcgtgaaa
    20701 ttgtcatcaa gaagatcatt tatctccttg attttatgct tattgttggt tgcgaataca
    20761 agttccataa tctgaagggt aaaagacaaa attaatcatt gcatcgtgat gcagcctttc
    20821 aggcatacca aaaccggaaa atattagttt attttggtag agatcaccag attaaaatcc
    20881 tctatggtta tcagccattt ccgcgggttc aaaatggttt gcaccctgct ctgtgcgggt
    20941 gtattcacag ctgtatctgc ggccgtgtct gtggccatgt ctgaatctgc gtctgtgtct
    21001 gcatccgcgg ccgtttctgt atctgcatcc gtggccgtat ctgcgacttg tccgggtcag
    21061 ggttcaggca tgttgtctga ggcttatcct ggtcaggggc ccggtctgca gtctgaggca
    21121 tgtcctgatc aggtgcccgg tctgcagtct gcaaggagtt tgaccgtcat gaacactgca
    21181 ctgtcagcag gcggcgtttc cgctcaatca atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnaccgtcat gaacactgca ctgtcagcag gcggcgtttc cgctcaatca
    21361 ataatccggc cttcaggcag gttgaccggc atttacctct atcagtcagg gcatatgatt
    21421 gacacggcag agactgtacc tgtcctgaga taccgtgacg gaattgtatt caccatggag
    21481 gatgatgcgc ttgttgagta ctcctatttc atggctgggc ctgacagtga atggactgta
    21541 tggactgaca gggcatacaa ggagtacacg aaccttgacg cgggaagata caccttcaag
    21601 taccgttaca gggagaaagg cgccggagac agcaacggcg ggggttcatt cagtttcagg
    21661 gtcaggccac cgtggtattt ctcactcctg gcgctgatca tctatccgct tttgctgatt
    21721 gccgggggta tcatgatgcg aagacggacg cacaggctct acatggaaag acagagcagg
    21781 ctcgaacagc ttatagcgga gagaacggaa gagcttcagc atgaaaagga gaagtctgac
    21841 agcctgcttg ccaatatgct tccgaagggc acggctgagg agataatgtc aaaggggaaa
    21901 gccgataaga gaaaatataa ttttgtcacg gtgctatttt ctgatatcca gggatttacg
    21961 aagatagctg aagaaatgaa ccctgagaca ctgattgatg agcttgatcg cttcttcttt
    22021 catttcgatt cggtggtgga aaaataccgg atagagaaaa tcaaaacgat tggagacgct
    22081 tacatgtgtg ccggaggcat cccggagcgg aacaggacaa atccggttga ggtagttctg
    22141 gcagcgcttg agatgcagaa gtacatgcag gaaatgaaga gtgatccgtc gaggcctgca
    22201 gcccgattct gggacatcag ggtaggaata cataccggaa cggtagttgc aggtgttgtc
    22261 gggcataaga agatcacgta tgacatctgg ggtgacaccg taaacactgc cagcaggatg
    22321 gaatcgtccg gagaggcagg aaagatcaac atctcaggca ccacttacga atttgtgaag
    22381 gagtttttta catgtgatta ccgtggcagg atgccggtga agtacaaggg tgaccttgac
    22441 atgtatttcg tcaccgggat aaggcctgaa ctaagggagc ctgacggatc tccgaacaga
    22501 aagtttcttg caaggattga gatgatacgc attctcgatg tggaggagca tgtcttctcc
    22561 aggtactctg aaatcgcttc tccggacctc ttctttcaca gcgctgatta tatacggagc
    22621 atatcattgc aggctgacct gcttgccagg gctgagaatc ttcctgacga ggaatacatt
    22681 catctgaggc tggcttctat tttcatatac ttcggttatg cttttgatta caatgatcct
    22741 gaggctgctg ctctgaaaag agccggtgag atattgtcat tttatggttt tggggcgcac
    22801 acactcgagg cggtaaggga gcttattgcc tcggcttctt caccggaaca gaccggcatg
    22861 gccggccgtg tgcttcatga tgctgtatat gattttaccg gaagagttga ttttatttcc
    22921 atggttgata aactctacag agaggagaaa gcctacggga agacaggtga tccgcaagtc
    22981 tggttcagtg agctggcttc caggatcggg gaaaaccggt tcctgactga aacagcacgc
    23041 aggctgaggg ctgtggctga ggatgagcag ctcagggccc tccgggagtt tgccggcgga
    23101 cggcttgaca atgcttaaaa tcatttgata ctttggtttt aaaatgtaca tttgggaaac
    23161 ccaaaattta agatcatgac aaatttagac tggaacaatc ttccatttgg ttacattaaa
    23221 acagatttca acgtaaggtg ttactataaa aacggccagt ggagcaagcc agaggtttcg
    23281 gattcagagt acatcaatat ccatatagcc gcaacagggc ttcactatgg tcaggaggcc
    23341 tttgaaggaa tgaaggcata ccgtggtcgt gacggtaagg taagattgtt ccgctgggat
    23401 gagaatgcaa aaagacttat atcatcggca gacggaatcc atatggctcc ggtgcccatg
    23461 gagctgttca gggaggctgt tgtcaagacg gtgaagctga atgaaaagtt tgttcctcct
    23521 tacggttcgg gtgcatctct ctatatcagg ccgctgttgt acggaagcgg tgcagaagta
    23581 ggtgtaaagc cggcatcgga atataccttc atagtctttg taactcccgt aggcccatat
    23641 ttcaagggag gcgtgaagcc ggttgacatg cttatctgcc gcgatgttga cagggcagct
    23701 ccgctgggca cgggcaacat caaggtagga ggcaactatg cagccagtat gagggccatc
    23761 gttaatgccc gtgagggtgg ctatggcaat gcgctcttcc tcgatgccag ggaaaagaaa
    23821 tacatcgatg aatgcggtcc ggccaacttc ttcggaataa agagcaatac ctacataacc
    23881 ccgaagtcgg aatcgatcct tccttcaatc accaacaaga gccttattac cattgctgaa
    23941 agcatgggga taaaaaccga aaggcgcagg attcctgttg aagagcttgc tgagtttgag
    24001 gagacaggcg catgcggcac agcagccgtt atcagtccga tagctaaaat atttgatccc
    24061 gataccggga aggtttatga gtattgcaaa gacggtaaac cggggcctgt ttcgatgaag
    24121 cttttcaaca agcttgtggg gattcagtac ggtgatgagc ctgatgaatt cggctggatg
    24181 accgaggtgt aagctttacg ctgcacaccg gttattttat cttttaagcc taaatatcaa
    24241 aaaattgcat ggctgctgat ggaattggcg gtttgtatgc tatttttgag acaaattttt
    24301 taaaatggca aaatcgacgg gagggatatc caggtacaga ctaggcagtt catatgtcac
    24361 gctcgttgta agtgtttccc tggtactctt cctgctgggc atccttggca tggtgcttat
    24421 caatgcccgt cagctgtcag attatttcag ggagagtctt tcgtttacca tcatgctgaa
    24481 ggaagacgca cgtgaggctg acatcaggat gttgcagaag gagcttgatg ccaagcctta
    24541 cgtcaagctt accagatatg tttcaaagga tgtggcggca atgaatctgc gcgaggaact
    24601 gggtgaggat ttcctggatt ttttgggata caatccgttg atgccgacga ttgacgtata
    24661 tcttctggca gagtatacac aacctgacag tgtgataaag attgaaaagg taatctctga
    24721 atacccggtt gttgatgagg tttattacca ggaatccttc ctgaacctga tcaatgacaa
    24781 tttccggaga ataagtgttt ttctgctggt aataagcttc ctgctgtttc tgattgccct
    24841 cacgatcatc aataacacaa ttcgtctttc gatatattcg aaaagattcc tgataaacac
    24901 gatgcagctg attggcgcaa acaggtcatt tattcggaag ccattcctgc ttcggagtct
    24961 ctttttcggc ttccttgctg ctctgattgc catcgggctg cttatggggc ttatgtatct
    25021 cgttgaaaaa gagttcttta ccctgtttac ctatgaaaac ataaatctgt ttctccttct
    25081 atgcctggcg ctggttgtgg caggggtgat tattaactgg atttccacat tttttgccgt
    25141 gaacaggtat cttggcatgc acgaggataa attatattat taagaaggat atggtcgcta
    25201 aaaaggaaga agaaaagaag accggtttcg ccctggggcg ggagaactat aagctgatgg
    25261 ccatcggctt tgccgttatc attgtcggtt tcattcttat ggccggaggc agatctgatg
    25321 accccaatgt tttctcggat aaaattttca gtttccgcag ggttacattg gctccgctga
    25381 tagttctggc cggctttatt tttgagatat atgcaataat gaaaaggcca agggattaaa
    25441 gccctggttc ttctttgatc aaataaccgg aaatgaattg gtatgaagcc ctgatcttcg
    25501 ggctggtaca gggattgact gagtttttac cggtcagcag tgacggtcat ctggagattg
    25561 taaaattcct gtttggaggg gtagaggaga gttttctctt ttctgttgcc gttcatggag
    25621 ctaccgtatt gagtatactt gcggtgttct ggaaggagat tctgaaactg ctgaagggtg
    25681 tattcaaatt tgaaatgaat gaagagacag tctacaccct taagcttata gtgtcaatga
    25741 taccggtggc aattgtaggt ttcaccatga aggagcaggt tgagtcactt tttattgctg
    25801 acatggatat tacggggtca tttctgcttg tcacggcact ctttctgctg attgggcatt
    25861 tcgtgcccaa aaggaataag cctatcagct atggcaatgc atttgtgata ggtatagcac
    25921 aggctctggc tactcttccc gggctttcca gatccgggtc tacaatttct accggtctga
    25981 tgcttggaaa cagcaaagac gaactggccc gcttctcatt cctgatggta atagttccga
    26041 ttctgggagc caatttcctt gaactggtaa ccaccgaaac cacagtagta agcggtgtgc
    26101 ttgtcccgct tgttattgca tttgttgcag cgtttgtgtc tggctatgta gcctgccgct
    26161 ggatgataaa cattgtcaga aaggggaagc ttgggtggtt tgcactctac tgtatcctgg
    26221 taggtctttc attaataata tttgcctgaa ggtgctgaag aggtgttccg actttgaaga
    26281 tggagaggta ctgctgtttc agaaaccggt ttactggacc tcctttgacc ttgtgaataa
    26341 ggtaaagctg gccatacgtc atcattgtgg aataaaaaaa ctgaaagtag ggcatgccgg
    26401 cactctcgat ccgctggcaa ccgggctgat gattatttgc accggtaagg ccacgaagag
    26461 gatgaatgag tttattgacc tcgataagga gtatattgcg gtgatcaggt tcgggcagac
    26521 aacaccttct tttgatctgg aaagtgaggt gaatgcagag taccctgccg gtcatataac
    26581 tgaggggctt atcagggaaa agctgaaagg atttttagga gagcaggagc aggagcctcc
    26641 gcttttttcg gccaagtttg ttgacgggaa gagagcctat gagcttgcca ggaagaagat
    26701 cgagcacaaa cttgaaagtg tgaaggttgt tttccgtgag atggagctga ttgattacag
    26761 cggggttgat gccactataa ggattgtctg cagtaagggg acctatatca gggcattcgc
    26821 ccgtgatctg gggcaggcgc ttggcagcgg tgctcacctg aggtcgcttg ttcgcagcgc
    26881 cataggcgat tttcgccttg aaaaggcact ttcggtagaa gaattcaaat tttttcttga
    26941 aaatttgaaa cataatgaaa actcatacgt ataattactt ttgcactaac ctttttcgat
    27001 catgaaacta tctcaattca agtacaccct tcctcaggaa ttgattgccc tttatccctc
    27061 gaaaaacagg gatgagtcgc ggttgatggt agtgcacatg gataccggag agattgaaca
    27121 taaggtgttt aaagacatcg tggaatattt cagggagcat gatgtgcttg tattcaacaa
    27181 caccagggtt tttccggcga ggctttatgg gaacaaggag aagacagggg ctgagataga
    27241 ggtgttcctg cttcgcgagc tgaaccgtga gcagaggctc tgggatgttc tggttgaccc
    27301 ggcaaggaag atcaggattg gcaataagct ttatttcggg gaggatgaca tgctggtggc
    27361 ggaagtgata gacaatacca catcgagggg cagaacactt cgttttctct ttgacggacc
    27421 ctatgaggag ttcaagaaga ctctttattc gctcggcaag actccgcttc ccaagttcat
    27481 caacaggcct gttgagccgg aggataatga gcggtaccag accatttttg caaagcatga
    27541 aggagcggtt gctgcaccga cggccgggct tcatttcagc agggaactgc tgaaaaggct
    27601 tgaaataatt ggcgttgagt ttgccgagat cacccttcac gtgggacttg gcaatttccg
    27661 taatgttgat gtggaggacc tgaccaaaca caaggttgat tccgagagga taatcatctc
    27721 cgaggaggca tcggcgctga tcaacaaggc caaagagagg aaaaacaggg tctgtgcagt
    27781 aggaaccaca gtggtacgaa ccctggagag ttctgtttca acctcgggaa tggttaaacc
    27841 gtttaacgga tggacaaaca agtttatctt tccaccttat gaattcaagg tacccgatat
    27901 gcttgtgagc aacttccagc tcccctattc aactctgctg atgatggttt cagcttttgc
    27961 gggctacaat cttctttttg aagcatatcg tactgctgtc aaggaaaaat acaggttcgg
    28021 aacctatggt gatgccatgc tgattctcta gcatataagc atcaaaatca ttgataaaca
    28081 cgtgattatg tagataattg gtgtcggtat catttgccgg cacttttctt tcccctgaaa
    28141 ttcacaaatt cagtcactta accactgcaa tgaagtaacc tttttgacgt ttgatgagta
    28201 tagatgacgt acagcagtta aagaagccac gtaatcaacc tgtagaatgt taaattacaa
    28261 aagagcgata ttttcattgc tgggaggaat actcgcttta ttatttgagt tatcagtcta
    28321 tggtcagtgc ggaccaggga aaaaggctat gccaattcag ggcaccggat caatcgtgac
    28381 tcaaaacgtc ggtgttgaac tgcctgagaa tatactggga aatcctgatg gaacaggagc
    28441 acagtttcat gataatggag actatattgt tgttgatttt attgatacaa tacttgccgg
    28501 acaatcttat gtaataacct ggagacaaag tccgtcaact ccaggaactt caagactcta
    28561 ttggagggag gctttggtag ttggtacata tatggctccc agaagtatta ctacaaccaa
    28621 cgaacagttc gcaagcgtta ttcttactgc aaacagcgac accaggtatc ttcgattata
    28681 caattctaac ggaattgact ttgaggttga tgctgtaact tatactacaa ctaaatgttt
    28741 ttctgatgat tgcgggggta atatgtacct gcagatcctt tccgggaatg ccttgcctcc
    28801 cggagccgga gataatcatg gtgttcaaaa tgcaagtgat attgcaggtg taccagatgg
    28861 aaagtatggg attttcgaca gcaatggcga ttgggttatt ctgaattttc cttatactgt
    28921 tcctgccggc cagcagtatt ctattatctg gagacctttg gacatgacag gtgcatcatc
    28981 ttccactatg gccattcagg agtctgatgg tggtacctgg ggtggtttaa agaccctgac
    29041 tccttattcc ggagaatcgc caatttttct cacagaggta gtgacagctt ctcagaacac
    29101 aaatcggata aggattcgga taagtcccgg atcagattat tttcacattg atgctgttgt
    29161 ttttaacgtg gcaggctgcc agcctccccc gccagtgctt aatgtttccg gaaaccttgt
    29221 gtattgtgac aatgccattc aaatctctcc gggattaatt atcgatgatc catatgatca
    29281 gatcatccct gctgtttatg ttcagataag cgacggtttc caggcaggtc aggatgtatt
    29341 gagttgtact ccagattttg gtattacacc cacttatctt tcggcttacg gcctgttgat
    29401 tttaaagggt ccggcaacaa cttcacagta tcagagtgta ctaagaactc tgacatacag
    29461 taacagcagc ccttcccctg ttgccgggaa caggaatatt attatttctt tcgagaggta
    29521 taatccaata acaggacatt actacagata tcttccaacc ccgggtctga gttggcatga
    29581 ggccagactt aacgccggca ggactcacct ttacggaatg cagggttacc tggttaccat
    29641 cggatcagct gctgagaata cctttctgcg atcgcagatg gccggaggtg cggtgtggac
    29701 aggggccagc gattcatttt cagcagaagg agactggaaa tggattacag gacctgaagc
    29761 cggaacttcg ttctggctcg gtgatgaaaa tggggcagag ttgacatatg ccaactgggc
    29821 tgctggtgaa cccaataata atgacgatga ggattttggt cattatctga gtgatggtac
    29881 atggaatgac attggttatt tcccgggatc tggcatttat gcatctgaag gtttctatgc
    29941 agaatttgga gatatgccag gtgatcctgc acctgacatc ttcggaactg ttactgtaaa
    30001 cgttgttact gctgagtctc tcaatccggt tattaccggt cccgatactg tttgccccaa
    30061 tgccacagga caggtttatt caactcccaa tgtgccgggt gcttcttact tgtgggaagt
    30121 gactggcgga attatcgccg aaggtcaggg cacaaattca ataactgtag actggggtgc
    30181 taccagcccg ggaatcgtca gggtaacaga aagtgcaggt gcttcatgcc aggttacaac
    30241 tgctgatact gtgataattg cagatatgat caaacctgtt atcaccggca t
//
LOCUS       DAOPBK010000004        29978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_1687_length_29978_cov_6.131371, whole genome shotgun sequence.
ACCESSION   DAOPBK010000004 DAOPBK010000000
VERSION     DAOPBK010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1687_length_29978_cov_6.131371"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2591)
                     /locus_tag="PLA17_00665"
     CDS             complement(<1..2591)
                     /locus_tag="PLA17_00665"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPK83953.1"
                     /translation="MKIVLLTIFFFFSLLLYPQEYKKREYIASRTEKPPVINGELDDG
                     EWAEGEWAGDFWQYSPVEGGPVSQNTEFKLLYDDYNIYVAIKAWDTAPDSIISRMTRR
                     DDIEGDEVAVALDSYFDQRTAFQFSVSAAGVKGDLMWFDDGLTQDDSFDPIWYVKTGI
                     YDWGWAAEMRIPLTQLRFSASEQQVWGLEVMRQIYRHNETVMWQPIARNASGLVHNAG
                     LLKGLTNIRPRKLFDLTPYGVARVETYEGEHGNPYHDGNDIKGNIGLDAKIGVTNNMI
                     LSMSLNPDFGQVEADPSEVNLTAFETFFSEKRPFFIEGNNITDYNLGLGSGGRGNDNM
                     FYSRRIGRQPRLSHSPGEGKYACTPAFTPIIGAAKLTGKSAGGISVGFIEAVTANVNT
                     RIYDPLTDKTTFRSAEPLTNYGVGRVQKDFSGGKTIIGGMVTSTIRSLDAETEDYFHK
                     SATSAGIDFTRYFGNMNYIIRLRTAFSNVTGTDKMIARTQKSVIHNFARPDADYLNYD
                     ITRTSLSGFGGNLMAGKIGGNWQFIYLSSWKSPGFEINDIGYMQMADMYMGVGVINYS
                     IYKPFSIFRSMSFGTNLIHLMDFGGNTNATAVSQSWTANYQNQWRTFLSGQLTRPRTD
                     NHLLRGGPAMKMPGQLYLSASIDSDWRKKLSGELDFSVTRTFDNVMTSCELSFELEYR
                     PFNALTLNIEPAWSRTVNMLQYVTLRYHQGIINQPERYIFGSIDQKIYSISIRTDFNI
                     TPDLTIQYWGQPFFGSGNYSGFKRITDPVAASLDDRYELLDGGVITYAIKERKYIIDE
                     NLDNSPDYNFSNPDFTVSEFLHNLVVRWEFLPGSTAYLVWSQSRDHYTGDGRYSLSQQ
                     SYNLFHGT"
     gene            complement(2727..5369)
                     /locus_tag="PLA17_00670"
     CDS             complement(2727..5369)
                     /locus_tag="PLA17_00670"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPK83954.1"
                     /translation="MKKFAFIVTALIVCTLAGTAVPLQDSIPKKTYRAQFISSPPVID
                     GSIDDAAWQTGEWQGGFTQIEPHNGAAATQKTEFLLLYDDNNIYVAFKVYDTSPDSIV
                     RRISRRDNGDGDFVGVGFDSYHDLQTAFAFITNAAGVKNDFIWSQDGRMEDDTWDPIW
                     FTAAKIYDWGWAAEMRIPLTQLRFRISDDGVWGFEAFRKLFRNQEISMWQHIDRNASG
                     VVHNFGTLTGLTGIKPRKQADITPFIVASLETFEREDGNPFATGINRRYNGGFDAKFG
                     LTNNMTLDLTVNPDFGQVEADPSEVNLTAYETFFEEKRPFFIEGKNITSFITGLGDGD
                     LGSDNLFYSRRIGRSPHLGVKTDNDEFAKVPRVTPIICAAKLTGKTPDGLSIGIVEAV
                     TAEGKAVIDSLGHRREQTVEPLTNYLVSRVIKDFGGGRTIIGGAFTNTRRFLDNTGID
                     GLVRNANTAGIDFIRYFGEERKWYLSATAAGSNLTGTKRAIENLQRSSTHYFQRPDAD
                     YVEVDPERTSLNGFGGNVQAGKIGGRWNFMAFTYMKSPGLDLNDVGYLQRADEIISGL
                     WSAYRFDKPFSIFRQIRPNTNFWTGWDFGGARLSSGGNLSLYVQFKNLWWTNFGVNYE
                     GRSLSNNLLRGGPAMLCPSSLNGWFNLGTNRSRKVSLSLYANASRGAENVSERYSAGI
                     DLTVKPGQSFTASIEPSWSINRNELQYVTCAGDNKRYILSRIDQQIVSMSVRLNYNIT
                     PDLTVQYWGQPFLAAMDYSRFKAVTDPRAKTLAGRYHLFSEDEMTYDADNGIYLVDED
                     LDGITDYTFANPDNNYDQFLSNLVFRWEFSPGSALFLVWSQTRSYSDDTGSFSISQNL
                     DNLYTSKKPYDVFLVKFTYRFGLR"
     gene            complement(5633..6124)
                     /locus_tag="PLA17_00675"
     CDS             complement(5633..6124)
                     /locus_tag="PLA17_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HPK83955.1"
                     /translation="MNKLRGQPELYQLLDSLKIPYDYIEHPPVPTVEEAMKYWSDIES
                     GHCKNIFFRNHKGNRHYLVILYYDSKLNIKELEQKLRQGKLTFASDRRLMQYLGLTPG
                     SVSPFGLINDHTRHVHLFIDKNLMNFERLSFHPNTNTASLIIPREGLFRFLEHTGNTW
                     EFI"
     gene            complement(6121..6525)
                     /locus_tag="PLA17_00680"
     CDS             complement(6121..6525)
                     /locus_tag="PLA17_00680"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPK83956.1"
                     /translation="MKSFIHKATDLTPEIILAPAENRFIIAGKSAPEDVRGLYYPVIE
                     WMEEFVAETKKGHNYTDANPFRLKLDLEYFNSSSAKFLFDIFEQIKEMSNDGIPVEIE
                     WYYDEEDEDLREAGEDLALLAELKFSYFPKTR"
     gene            complement(6586..7068)
                     /gene="rnhA"
                     /locus_tag="PLA17_00685"
     CDS             complement(6586..7068)
                     /gene="rnhA"
                     /locus_tag="PLA17_00685"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPK83957.1"
                     /translation="MSRPVVTIYTDGACSGNPGPGGYGVVLLSGSLRKELSGGFRLTT
                     NNRMELLAAITGLEALRLDGLKVKLHTDSQYLVNAVNNGWLFNWEMKRFAKKKNKDLW
                     IRFLEVYRRHKVTLIWVKGHSNDPENERCDTLAVAASKLPGLPEDEGYRANSDDEELI
                     "
     gene            complement(7065..7643)
                     /locus_tag="PLA17_00690"
     CDS             complement(7065..7643)
                     /locus_tag="PLA17_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPK83958.1"
                     /translation="MQINFLDILACFMVLFAIIDMPGSIPIILDIKSKTGNIHPWKAT
                     LVSFLIFLAFLIFGEKLLGIFGIDVGSFAIAGAFIIFLIGMEMVLGVEFFKHDSPGGG
                     TIVPIAFPLIAGAGSITTILSLRAEYATINILIALILNMVIIYTVLRLTSRIERLLGP
                     TGLHILKKFFGIILLSIAIRLFINNTGIILPQ"
     gene            complement(7980..10124)
                     /locus_tag="PLA17_00695"
     CDS             complement(7980..10124)
                     /locus_tag="PLA17_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPK83959.1"
                     /translation="MKKFIAIIVILVTFSASQARADEGMWLLPLIEKLNIGTMTGMGL
                     KLTAEDIYSINQASIKDAIVIFGGGCTAEIVSPHGLLLTNHHCGYGQIQNHSTVEHDY
                     LNNGFWAMSKEEELPNPGLSVTFLVRIEDVTSRVLGKVNAEMTEAARREAILAESAAI
                     TAEATKGNHYNARVSSFYSGNQYFLLVYEVFSDVRLVGTPPNSIGKYGHDSDNWVWPR
                     HTCDFSVFRVYMGPDGKPAQYSKDNVPLKPKHYLPVSIKNLQKNDFAMVMGYPGTTTR
                     YMTSWEVDQAMKITNANRIKIRGARQEIWMKDMMADPKVNIQYASKYSGSSNYWKFSI
                     GQNAGLARLRTAEKKAAFEAEYMKWVNADPARKAKYGDALSLIENAVKGRAEKYNVSQ
                     YVREALSTELINFAGQMTMLEDALAKRDTEEIDSTTERLKRFAENFYDDYNYPTDQKT
                     TKTMFKLYRSDIDPKYWPSFYRLIDTKFKGNIDAFVDNIFAKSIFVSPEKLDAFLEAP
                     SLKVLKKDPAFVVAKSISEMSSTLMNDLQEFSSDLSKGQRLYIAGAMEYQPDKTQYPD
                     ANSTMRLTYGKVLDYYPYDAVHYNWITTLDGVVQKYKKGDYEYDLPQRLIDLNEKREY
                     GRYGSPDGYMPVCFITNNDITGGNSGSPVINGNGELIGLAFDGNWEAMTGNIAFEPDL
                     QRCISVDIRYVLWVIDIYSGAGYLLDEMDIRE"
     gene            complement(10201..10803)
                     /locus_tag="PLA17_00700"
     CDS             complement(10201..10803)
                     /locus_tag="PLA17_00700"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPK83960.1"
                     /translation="MLKPFFSNGIREAGCDEAGRGCLAGPVYAAAVILPKRYRNKELH
                     DSKRLTARQRERLRTEIMSSAITWCIASCSNTEIDEMNILRASITAMHRAIDGLEPRP
                     LLLLIDGNRFYTYRNIPHQTIIKGDATYLSIAAASVLAKTERDHHMEELDALYPCYGW
                     RKNKGYPTPEHRRAIAEFGPTPYHRLSFNLTDPQGKLLFL"
     gene            complement(10803..12035)
                     /locus_tag="PLA17_00705"
     CDS             complement(10803..12035)
                     /locus_tag="PLA17_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPK83961.1"
                     /translation="MIIAVNARTLRAVPRDGIGWFTLEVVSGMVRNHPEHRFVLIGDR
                     RYGRLPAEGNRQHEQATAEGNRQDEQAPAADARASGILPVEGRNVEYVTIPLRTVHPL
                     LWHAWHEYLLPPVLKRIKADLFIAPDGMIPLRTEVPCIPVIHDLNHEHRPGDIPGGEC
                     RYYRRRFPLFAAKGARVATVSHFSAGDIAATYGISPDKIDVVPNGVAAVFSPPQPGEA
                     EETRRQYTGGRPYFLFVSNFSPRKNVEAVIRAFEIFRQSGGGEHVLLLAGRRLYLTRE
                     MDKHLELSPYGRDILFTGSVTRDVLRRLYGAAEALTFVPWLEGFGIPVVEAQRCGTPC
                     ILSDSSSLPEVSGGAALCVSPADAGAIAEAMARLVNDGALRRRLADEGYANSLRYTWE
                     QAAEEMWKSVIKATGGAA"
     gene            12118..13569
                     /locus_tag="PLA17_00710"
     CDS             12118..13569
                     /locus_tag="PLA17_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPK83962.1"
                     /translation="MRRKFLINITFLLTVSILVKAFWVLGIDRTVQNVVGETAYGNYF
                     SLFSFSVLFTLLLDMGLSGFNNRAVSADPARVRIYFGNVLVIRLFLTALYFLVTISVA
                     YALGYRGEQITILLVLMLNQVMASMIIWLRSSISGMQYLFIDSLLSVADRLVMIIICS
                     VLLWGGVTAGGFRIEWFVWAQTAAYFTVMCTAFIIVVYKGKVAAVKPDISVLKSIIMT
                     GLPYSVVVFAMTLYWRIDSVMIERLLPDGATRAGNYAQAFRLFDALAMIPVLFGGILL
                     PIMTRGLSSGSDIRPLARMACRMLMVPLGIGAVTLATFPAEMLDMLYTSPAGDAVTAF
                     MILMFALVPVGVIYIYSTMLTASWKLKMLGVITVSGMIISLSLNRILIPELGPAGAAS
                     AALVTQSLVAVACMLTVRRTMSGIAGNGRILLFLLMLGLTFFTGWLLRRTGIPWIAAA
                     ALELATGILLALLFRFIVPLSGIRLITGRFGTE"
     gene            13583..13774
                     /locus_tag="PLA17_00715"
     CDS             13583..13774
                     /locus_tag="PLA17_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83963.1"
                     /translation="MRLYETVARDNNGREPANTPAEERFPIAPDSSTGLTDHPVDFKL
                     SGLSLNAFFIYLCAKFQMT"
     gene            13771..14863
                     /gene="prfB"
                     /locus_tag="PLA17_00720"
     CDS             join(13771..13833,13835..14863)
                     /gene="prfB"
                     /locus_tag="PLA17_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131840564.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPK83964.1"
                     /translation="MITNDQIQDLVERRDALRRYLDVDARLNEIREKEEITRSAGFWN
                     DPKKAEELLKEISSVKSWTTAFATVDTAVEDMLVFYDFFREGEVTDEEMGQQYDKTLA
                     EVEKLELRNMLRREEDRLGALLKINAGAGGTESNDWARMLMRMYLMWGEKNGYKITEA
                     YYQAGDEAGIKTVTLEFEGENAYGYLKSENGVHRLVRISPFNAQGKRQTTFASVFVAP
                     LVDDDIVVEINPSDITWETFRSSGAGGQNVNKVETAVRLHHAPTGIVIENQESRSQGQ
                     NKENALRLLRSHLYEMELRKKMEEQAKIEGAKKKIEWGSQIRSYTLHPFKLVKDLRTG
                     CETGNAQAVLDGEIDEFIKAYLMEYGGKE"
     gene            14935..16800
                     /locus_tag="PLA17_00725"
     CDS             14935..16800
                     /locus_tag="PLA17_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPK83965.1"
                     /translation="MMDKKKKLFEQFPPVSTTEWMEKVTADLKGADFTRRLVWKTRDG
                     LSVMPFYREEDLDKLPHSNLLPGDFPFVRGVEVMGNNWLIRQDITVTDYKAANAKALD
                     ILMRGITSLGFVIEDSQSVTPDTVAELLKDIHPESIEINFSVAGGAKELVAALKGYLS
                     SSGAALKQVKITVPADPLGRLAARGRLCVPVDQGLDYLADLVSDAAEIPGIRCVEPSG
                     TLFSNAGAGPVAELAYTLSLGNEYMAALTGRGTDPDTAAKAFRFTFGIGPDFFPEIAK
                     LRAARMLWAEIVSAWGTKESGSALMHIHSVTGRWNKTLYDPYVNMLRTQTEAMSAVLG
                     GAESVTVEPFDTVFRSANDFSERIARNQQLLLMEEAHLGKIADPGAGSYYIEELTSMI
                     VSEAWKLFLEIEEEGGFLAALRKGMIQQRISEAAAKRKADMARRKEVLLGTNQYPDFT
                     EDKAPEHDPGKLVTEQTPSSEEVMPVIPARGADEFEKLRLATEKSGRRPLVFMLTIGN
                     LAMRRARAQFSANFFAVAGYEVRDNNGFASVAEGVTAALEAKADIIVICSSDDEYATL
                     AKEAFTATGRKALFVVAGNPPCMEELRAEGVEHFISIKSNVLETLQMFNRILGIN"
     gene            16807..18963
                     /gene="scpA"
                     /locus_tag="PLA17_00730"
     CDS             16807..18963
                     /gene="scpA"
                     /locus_tag="PLA17_00730"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPK83966.1"
                     /translation="MSKRPKFNDIDVLKVTVPSRDKQKWEKENGISPQWETPERIILK
                     SVYSREDLKGMEHLHYAAGIPPYLRGPYSAMYAMRPWTIRQYAGFSTAEESNAFYRRN
                     LAAGQKGLSVAFDLATHRGYDSDHERVVGDVGKAGVAVDSVQDMKILFDQIPLREMSV
                     SMTMNGAVLPIMAFYIVAGLEQGAKLSELQGTIQNDILKEFMVRNTYIYPPAFSMRII
                     ADIFKYASGNMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGVNAGMNIDD
                     FAPRLSFFWGSGMNFFMEIAKMRAARMLWAKIVSQFNPKNPKSLALRTHTQTSGWSLT
                     EQDPFNNVGRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETG
                     ICKAVDPWAGSYYVETLTHEIAHRAWELIEEIESLGGMAKAIESGIPKMRIEEAAARA
                     QARIDSGAQTIVGLNRYRLDKEDPLEILEVDNSAVREAQLKRLAEVKAGRNEEDCRRA
                     LDAITRAAETGEGNLLELAVDAASKRATLGEISYACEKIAGRYKAVIRTISGVYSSEY
                     KSDSDFEQARAMAAEFATRAGRQPRIMVAKMGQDGHDRGAKVISTGYADIGFDVDIGP
                     LFQTPSEAARQAVENDVHLLGVSSLAAGHKTLIPQVISELKKLGREDILVIAGGVIPP
                     QDYQFLYDAGVAAIFGPGTSVAKTAKEILTLLLQTV"
     gene            18960..19613
                     /locus_tag="PLA17_00735"
     CDS             18960..19613
                     /locus_tag="PLA17_00735"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPK83967.1"
                     /translation="MMRTAFALLSVVAIALLAGCSAGRKNVAVAGEPSAAVAAEQQQP
                     EAQVPDVAPFGNAALAGKPEGDEGAAHVWDFRDATTWLLGEFLPGMLNNPPHSEWYHE
                     GYAGYQPDQEVMAELMEIEKDDLTITIVIGTWCPDSRREVPRFMKIADLWGFPHDRIK
                     FIGVDINKVAPLEDYFQLAIERVPTFIFYKKNIEKGRIIEVPETSLEQDTRNILKGN"
     gene            19635..20135
                     /locus_tag="PLA17_00740"
     CDS             19635..20135
                     /locus_tag="PLA17_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83968.1"
                     /translation="MEEEKSAGQSSGTGMGVGALVTAIIAFLLAVIPCVGLVAVIPAV
                     IAIVLAIIGLSRPQVNQGMLVGGLVVGIIALMISASQIFVLGKIAEKSGSWATNIEKV
                     IRNVADDIEDEFGDQEVTIKINKDDETVEIKSSGKRGDLEERLEELEGSVDTLKSRSD
                     TTKGEW"
     gene            20204..20548
                     /locus_tag="PLA17_00745"
     CDS             20204..20548
                     /locus_tag="PLA17_00745"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HPK83969.1"
                     /translation="MGYSLFYSPEEDEYPVPCIHEKLTGEPCPSCGLSHAFSLIVRGR
                     VDEALEWNSQSLRVFIFFALQLVMRSGLAVTAMWVKRNLRVVAVTDAVISGVMTLAAF
                     WPFIRMLWISLF"
     gene            complement(20585..21073)
                     /locus_tag="PLA17_00750"
     CDS             complement(20585..21073)
                     /locus_tag="PLA17_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPK83970.1"
                     /translation="MAEKKEMDELLQRAMRACSAREYCISDIEALLERWGADSAETRT
                     RIIGRLIKEKFIDEQRYSRAFVLDHFRHSLWGRVKISMALKQKKINPEAIASGMEAID
                     EDEYLQLLQKILSDQRRRIKAKNRLDLKGKLLRYALGKGFESHLVYDAINSLLCEGDD
                     AE"
     gene            complement(21066..21932)
                     /gene="prmC"
                     /locus_tag="PLA17_00755"
     CDS             complement(21066..21932)
                     /gene="prmC"
                     /locus_tag="PLA17_00755"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPK83971.1"
                     /translation="MDGKVQTISEIRGRITELLKAGWPAEEAAALASAIIGEYTGMGR
                     AAQMAFGERVPEAGAAARIENAVARAAAGEPLQYILGYTEFCGHRIEVAPGVLIPRPE
                     TEEMTMMIISENSGFTGTVTDICTGSGCMAIAMAMAFPRATVAATELSGAALEMARRN
                     ISSNGAAVTLIKSDILNDDPLQIPLSSIIVSNPPYITMREKAMMHRNVLDHEPHEALF
                     VPDDNPLLYYRAIAAVAETRLLPGGTLWLEVNENLAGETAALLSPKSYSEVRIIRDIR
                     GKERFIKAEKYG"
     gene            21958..23007
                     /gene="ribD"
                     /locus_tag="PLA17_00760"
     CDS             21958..23007
                     /gene="ribD"
                     /locus_tag="PLA17_00760"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPK83972.1"
                     /translation="MPRPDDLIWMRRALQLAAMAEGCTSPNPMVGAVVVHNGVIIGEG
                     YHLKAGTPHAEVHAINAVSDRTLLPSSTIYVTLEPCSHQGRTPPCSDLIVSSGIRRVV
                     IGAADTNPLVAGRGIARLRQAGVEVITGVAEEECRRLNRRFFTWHEKKRPYVILKWAR
                     SADGFIDRCRKESDPPGPTWITGMAERVLVHRWRAMEDAILAGGGTIRADNPSLTVRY
                     WSGRNPVRIIVSRSGNLDPDSSLFDGRAETVLYTCNGQAQFRNTRTVTLTGAEPFIGE
                     VLANLWQEGIQSLFVEGGAYIIGSFIESGQWDEARRFTGTASFGDGVPDPFPGFTPGE
                     TVLFDKSILEFAYHF"
     gene            23231..26473
                     /locus_tag="PLA17_00765"
     CDS             23231..26473
                     /locus_tag="PLA17_00765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPK83973.1"
                     /translation="MRGRGRNSGRSIIILALLLLSHVILIPRLSGQTFYFEQYGSKEG
                     LSASKVYTIIQDANEFIWLGTGSGLTRFDGLHFENFSTSDSLAPGGVKSICEDSRGRL
                     WLGHIGGGVSIIEDNIIRRVCFSPSIFIGVDSARIRDPLKPINSLTGDITGITEYGGS
                     IWVSTSRSGALKLDMPGHGVRVMTGQQYTGRQGLSNDAFGLYADHRENLYCITDVGIR
                     KYDHASDRFIPFHPEGLTRYFLTTTIFEDSRGNMWFGTFNGGLYRQDSETGKMGLFDT
                     RNGLWGNFVSYILEDYRGNIWVASMEDWESRGGITVFSPEGTVVYNNSNGLQAQHILC
                     MIEDKEKNILIADRDAGLFIYKGDHFASFMAPRFMPSNDVFTLAQDHTGRYWFGTGSG
                     LSLYNPALKEDDRVRLRGFPPSLEETQISIIRPDKEGNVWIATAGDEVYRYDTDDKNF
                     RYDLDLNASLRPLRITAMTTDQRNNLWIGTDGALIMWDPQLKKSTKYTRSDGLTGNNI
                     TALFCDSRGSLWIGTEEQKGLARLNPGNGTFITLTFRSDVHPTAITETPDGMIWVGTI
                     DGLYGLSNDTVKKKLTEAEGLLMNSINLLQPDRSGNLFIGTNLGLNRYDSNTGRISSF
                     TEKSGFTGIETRPNASFADDMGDLWFGTSNGAMRLSPSMLPPVVTEPATHIWRTLVNY
                     EVYPMHDGIRLLHTQNRIIFDFLSICLVNPGAVRYQFMLEGADKEWITTDQNRAVYSS
                     LRPGKYIFLVKASNNYGFWNEVPAKLSFSIRGPVYLTSWFIILCIAVAVAGVYLYISL
                     RERKLRREKEHLEQKVKERTAEVVSKSHELEEKNRDTTASIRYAERIQAAMLPPENYF
                     NDTFVLFMPRDIVSGDFYWMYDTGYKQYIAAVDCTGHGVPGAFMSIIGHNSLNKIVRE
                     SGIEKPSDILDHLNIEVVHALLQRSEKAVRDGMDISVISYDILEHTVEYAGAYQPLYH
                     VRDGKVTVYRGDRFPIGMIDGQARKSFTNNLVDVKSGDMLYIFTDGFADQFGGEHEKK
                     FKTLKIRSLLSEIYMFSVEEQKRRLEKAIREWMGELAQVDDILFIGTRVP"
     gene            complement(26552..27127)
                     /locus_tag="PLA17_00770"
     CDS             complement(26552..27127)
                     /locus_tag="PLA17_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPK83974.1"
                     /translation="MKVCVFAASSSRIDAAYMKAAKELGMAFARHRVHAVFGGGGIGL
                     MGIFADSLMANGGSITGVIPGFMMDEGWGHSGVSDMIVTPDMSERKKTIFAMSDAAVA
                     LPGGVGTLEELTEVITLKQLGQFNKPVIIINVNGFYDHLIEFFDHMVRGHFMRLEHKD
                     IWQIVTTAEEVMPAIVNYTGWYADPKTIARI"
     gene            complement(27135..27452)
                     /gene="trxA"
                     /locus_tag="PLA17_00775"
     CDS             complement(27135..27452)
                     /gene="trxA"
                     /locus_tag="PLA17_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPK83975.1"
                     /translation="MTQQVNDSNFDEVVLKSDKPVIVDFWAEWCGPCRMIAPYIEQLA
                     DAYRERALVVKCDIESSPGIARRFTIRNIPTVLYFKNGEVVDKQVGAAARSAFEAKLT
                     PLL"
     gene            complement(27499..>29978)
                     /gene="dnaE"
                     /locus_tag="PLA17_00780"
     CDS             complement(27499..>29978)
                     /gene="dnaE"
                     /locus_tag="PLA17_00780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPK83976.1"
                     /translation="IKNMKFPGYFLIVQDFLRAAREMGVWVGPGRGSAAGSAVAYSLR
                     ITDIDPIKYNLLFERFLNPDRISMPDIDIDFDEDGREKVLRYVVEKYGHDRVAHIITF
                     GTMAAKMAIRDVARVQKLPLPEAIRLTKMVPDKPGVTLKAAYEEVPELLKEKDSKDPL
                     VAQTLKYAGVLEGSIRQTGVHACGIIIGKNSLSEHIPICTAKDTDLYVTQYEGNHVEP
                     VGLLKMDFLGLKTLSIMKDAVENIKKSKGITIDLQTLELDDRATYELFSNGETTGVFQ
                     FESVGMKRYLRELKPNQFGDLIAMNALYRPGPMDYIPQFIRRKHGQEKISYVVPAMEK
                     YLKETYGITVFQEQVMLLSQELGGFTKGEADSLRKAISKKKLDIMNMLKEQFIKGCQA
                     NGYDQNTINRIWSDWESFAQYAFNKSHSTCYALIAYQTGYLKANYPAEFMAAVLSRNI
                     NDIKKITVMMEETRRMGIEVLGPDVNESLLQFTVNSKGNIRFGLGAIKGMGENAVLHL
                     IEEREKNGPYKDIYDLAERVNLNTVNKKMLEALVVSGALDGLPDMTRARYFAADGKGS
                     SFLESLIRYGNNVKNIRNSTQQSLFGDTGGFDLVRPEPAPCPDWSKLEKLNKEKEVIG
                     IYLSSHPLDDFKLEINTFCNASLADLQNLSEFANRDVCVAGIVSDTRSGVTKNGKPFG
                     GFTLQDYTDSFSFLLFDKDYVAFSNYFRNDYQLLVKGRVQGRHYKPEELEFRIKEIHL
                     LTAVREDLITSLTIKLKPELVNPEFIKNLKSVILENPGNKSLKFLLIDHDERITIPLF
                     SRSIKAGITDELIGWIEDNPELGFKVN"
BASE COUNT         7043 a         7666 c         7850 g         7419 t
ORIGIN      
        1 gtgccatgga agagattata tgattgctgg ctgaggctgt accggccatc acccgtatag
       61 tgatccctgc tctgtgacca gacgagataa gccgttgatc cgggaagaaa ctcccaccgc
      121 actaccaggt tatgaaggaa ttcacttacc gtgaagtcgg ggttgctgaa gttatagtcg
      181 ggagagttat cgagattctc atcaataatg tatttcctct cctttattgc ataggttatc
      241 actccgccgt caagcagctc atagcggtca tcaaggctgg cagcaacagg gtcagtgatc
      301 cgtttgaacc cgctgtagtt acccgagccg aagaagggct gaccccagta ttgaattgtc
      361 agatcaggag tgatgttgaa gtcggtcctt attgaaatac tatatatctt ctggtctatt
      421 gaaccgaata tatatctctc tggctggtta ataataccct gatgatacct gagggtcacg
      481 tactgcaaca tgttaaccgt ccggctccac gctggctcaa tattgagagt caaagcatta
      541 aatggtctgt actccagctc aaagctcagc tcacagctgg tcatcacatt atcgaaagtt
      601 ctggtaacac tgaaatccag ttcaccggat agtttttttc tccagtcaga atcaatgctg
      661 gccgacagat agagctgacc gggcattttc attgccgggc ctccgcgcag aaggtggtta
      721 tcagttctgg gtctggtcag ttgcccgctg agaaaggtcc gccactgatt ctggtagttt
      781 gctgtccacg actgggagac ggctgttgca ttggtatttc cgccgaagtc catcaggtgt
      841 atcaggttgg tcccgaagct catgcttctg aatatactga acggcttata gatgctatag
      901 ttgattactc ccacacccat atacatgtca gccatctgca tgtagcctat atcattgatc
      961 tcaaagcctg gtgatttcca gctcgagagg taaataaact gccagttgcc tccgatcttg
     1021 cctgccatca ggttccctcc gaatccgcta agtgatgttc gtgtgatgtc ataattgaga
     1081 taatctgcat caggccgggc gaaattatgg ataacagatt tctgtgtgcg ggcaatcatt
     1141 ttatcggttc cggtgacatt gctgaaggca gtgcgcaacc ggatgatgta attcatgtta
     1201 ccgaaatacc tggtgaagtc tatgccggcg gaggttgccg acttatggaa ataatcctcg
     1261 gtttccgcat caagtgatct gattgtgctt gtcaccatcc cgccgattat tgtttttccc
     1321 ccgctgaagt ccttctgcac cctgcccacg ccgtagttgg tgagtggctc tgcactcctg
     1381 aaggttgtct tgtctgtcag ggggtcataa atcctggtat ttacatttgc agttaccgct
     1441 tcgataaaac ccaccgagat gccgccagct gattttccgg tcagctttgc cgctccgatg
     1501 atgggagtga acgcgggtgt acaggcatat ttaccttcgc cgggtgaatg gctcagccgt
     1561 ggctgcctgc ctattcttct tgaatagaac atgttgtcat ttccgcggcc gccgctgccc
     1621 agtccgaggt tgtagtccgt aatgttgttt ccttcaatga agaagggccg cttctcactg
     1681 aagaaagttt caaaggctgt taggttaact tcagacgggt cagcctctac ctgcccgaaa
     1741 tcgggattaa gtgacatgct gaggatcatg ttgtttgtca ccccgatttt tgcatcaagg
     1801 ccaatattcc ctttgatatc atttccgtca tggtacggat ttccatgttc cccttcgtat
     1861 gtttccactc ttgccactcc gtagggggtc aggtcaaaca gcttcctggg cctgatgttt
     1921 gtgaggcctt tcagcagccc ggcattatga accagccctg aagcgttcct ggcaatgggc
     1981 tgccacataa ccgtctcatt atggcgatat atctgcctca tcacctccag cccccatacc
     2041 tgttgctcac tggcagagaa ccggagttgt gtgagtggta tcctcatttc agcggcccat
     2101 ccccagtcat aaataccggt cttgacatac catatcggat cgaatgaatc atcctgtgtc
     2161 aacccgtcat caaaccacat gaggtctccc ttgacacctg ccgcgctcac gctgaattgg
     2221 aatgcagtgc gctggtcaaa gtatgaatcc agtgccactg ccacctcgtc accctcaata
     2281 tcatcgcgtc ttgtcatacg tgaaatgata ctgtcaggtg cagtatccca ggcttttatg
     2341 gcaacataga tattgtaatc atcataaagc agtttgaact ctgtattctg gctgaccggc
     2401 ccaccttcta caggggaata ctgccagaag tcaccagccc attcaccttc tgcccattcc
     2461 ccgtcgtcaa gctcaccgtt gatcaccggc ggtttttcag tgcggctggc gatgtattct
     2521 cttttcttat actcctgcgg gtagagtaaa agagaaaaaa agaagaatat tgtcagcagg
     2581 acaattttca ttataaagga tttcagagac agtaaggtat acaaaagtaa aaaaaataga
     2641 tgaaatcata ttgcgattga aaaccttaat taaaaagagc ggtggattca attccatcgc
     2701 tcttcctgac ctgcaattag cggcgtttac cgcaaaccga aacggtatgt gaattttacc
     2761 aggaacacat catagggctt tttcgaggtg tagagattgt caaggttctg actgatcgag
     2821 aaggaaccgg tatcatcact gtagctacgg gtctgtgacc agacgaggaa gagggctgat
     2881 ccgggactga actcccatct gaaaacgagg ttcgaaagga actgatcata attattgtca
     2941 ggattggcaa aagtgtagtc agtaatgccg tcaaggtctt cgtcaaccag gtagatgcca
     3001 ttgtcagcat cataggtcat ctcatcttca ctgaagaggt gataacgacc agcgagggtc
     3061 tttgccctcg gatctgtaac tgccttgaac ctgctatagt ccattgctgc aaggaacggc
     3121 tgtccccagt actgcacggt cagatcagga gtgatattat agttcagcct cactgacata
     3181 ctgactatct gctgatctat ccggctcagt atatatcgtt tattgtcacc ggcgcatgtt
     3241 acatactgga gctcgttacg gtttatagac caggagggtt caatggaggc agtaaatgac
     3301 tgtccgggct tcaccgtcaa gtcaattccg gctgaatatc tctctgatac attttctgct
     3361 ccgcgcgatg cgttggcata gagtgacagg gatacctttc tggatctgtt tgtgccaaga
     3421 ttgaaccagc cattgaggga ggaggggcaa agcattgccg gtcctccgcg gaggagattg
     3481 ttggataatg acctgccttc gtaattaact ccgaagtttg tccaccacag gtttttgaac
     3541 tgaacataga ggctcaggtt cccgcctgag gagagacgag ctccgccgaa gtcccatccg
     3601 gtccagaaat tggtattggg ccttatctgc ctgaatatgc tgaacggctt gtcaaacctg
     3661 taggcagacc acaggccgct tattatttca tcggctctct gcaggtatcc aacatcatta
     3721 aggtcgagcc ccggagactt catataggta aaggccatga aattccagcg cccgccaatc
     3781 ttccctgcct gaacattgcc tccgaaaccg ttgagtgatg tccgttccgg atctacctcc
     3841 acatagtcag catccggtct ctgaaaataa tgtgttgatg agcgctgaag attttctatt
     3901 gctcttttgg ttcctgtcag attgcttccg gccgcagtgg ctgacaggta ccatttacgt
     3961 tcttcaccaa aatagcggat gaagtcaatt ccagccgtat tggcatttct taccaggccg
     4021 tcaatcccgg tattgtcaag gaaacggcgg gtgtttgtga atgctccgcc gataattgtc
     4081 cgtccgccgc cgaagtcttt aatgactctt gacacaaggt agttggtgag aggttcaacc
     4141 gtttgttctc tcctgtgacc cagcgagtca ataacagctt tgccctctgc cgtcactgcc
     4201 tccacaatac ctattgacag cccgtcagga gttttccctg tcagcttggc tgcacatatg
     4261 attggcgtga cgcggggtac ttttgcgaat tcatcattgt cagttttaac accgaggtgc
     4321 gggctccgtc ctatcctcct cgaataaaag agattgtcag agccgaggtc accgtcgccg
     4381 agacctgtta tgaagctggt aatatttttc ccctcaatga agaacggcct cttctcttca
     4441 aagaatgtct cataagctgt gaggttcacc tctgaaggat cagcctcaac ctgcccgaag
     4501 tcagggttga cggtaaggtc aagggtcatg ttgttggtca ggccgaactt ggcatcgaag
     4561 ccaccgttgt atctcctgtt gatgccggtg gcgaacgggt tgccatcctc tctctcaaat
     4621 gtctccaggc tcgccacgat aaacggggtg atgtcagcct gtttcctcgg ctttatgcct
     4681 gtaagcccgg tcagcgtgcc gaagttatgc accacaccgg aagcattgcg gtcaatatgc
     4741 tgccacatgg aaatctcctg gttgcgaaag agtttgcgga aggcctcaaa accccataca
     4801 ccatcgtcgg agatacggaa cctgagctgg gtgagcggaa tcctcatttc tgccgcccat
     4861 ccccagtcat aaatttttgc tgccgtaaac catatcggat cccaggtgtc atcctccatc
     4921 cggccgtcct gtgaccagat gaaatcattt tttactcctg ccgcgttggt gataaatgca
     4981 aaagcggtct ggagatcatg gtagctgtca aaaccaaccc cgacaaaatc accgtcaccg
     5041 ttatccctcc tggagatacg cctgacgatg ctgtcaggac tggtatcgta aactttgaag
     5101 gcaacgtaga tgttgttgtc atcatacaga agcaggaatt cagttttctg tgtagcagct
     5161 gctccgttgt gaggctcgat ctgggtgaaa ccaccctgcc attcaccggt ctgccacgca
     5221 gcatcgtcga ttgatccgtc aatgacgggt ggcgaggaga taaactgcgc cctgtatgtt
     5281 ttcttaggga ttgagtcctg gagcggaaca gcagttcctg cgagagtaca cacgatcagt
     5341 gcagtcacta taaatgcaaa ttttttcatg gcaataaagg ttaacatgag ggcgcaagtc
     5401 cgttagaaag acattgtgaa ctgcccgttt gctgcacacg gatgagtaat gtcagtaatt
     5461 aatgcttata atttcggtga atggcagagc ttgtccggtg aatggcgaaa gtgggaattg
     5521 aagcattagc ctgtcaggca ggagctgacc tttagcggag cttgtccggt gaacggcgga
     5581 agtgggaatt gatacagtga ccggtcaggc aggagctgac ctttaaaggt gatcagataa
     5641 actcccaggt gttgccggta tgttccagga atctgaagag tccctcacgg ggaataatca
     5701 gtgaggcggt gtttgtattc gggtggaatg agagccgctc aaagttcatc aggttcttgt
     5761 caataaacag atgaacgtgg cgggtgtggt cattgatgag accgaatgga gatactgacc
     5821 cgggagtaag accgagatat tgcatgagcc gcctgtcgga agcaaacgtg agtttgccct
     5881 ggcggagctt ctgctcgagt tctttgatat tcagtttaga atcataatag aggatcacaa
     5941 gataatgcct gttacccttg tgattacgga aaaagatgtt cttgcagtgg ccggattcga
     6001 tgtctgacca gtatttcatt gcttcctcaa ccgttggcac gggaggatgc tctatataat
     6061 catagggtat cttcagcgaa tcaagaagct ggtaaagttc cggctgtccc ctgagcttgt
     6121 tcatctggtt ttagggaaat aactgaactt aagttcagca agcagagcca ggtcctcgcc
     6181 tgcctcacga agatcctcgt cttcctcgtc ataataccat tcgatttcca cgggaatccc
     6241 atcgttgctc atctccttta tctgttcgaa gatgtcaaac aggaacttcg cggaggatga
     6301 gttgaagtat tcaaggtcaa gtttcaggcg gaacgggttg gcatccgtgt aattatgccc
     6361 cttttttgtt tccgcaacga actcttccat ccattcaata accggataat agagacccct
     6421 cacatcctca ggcgccgatt tcccggcaat gatgaacctg ttttccgcag gagcaaggat
     6481 tatttccggc gtcagatcag tagctttatg tatgaaactt ttcatctctt ccttttttta
     6541 caaatttgca ctttttttat ggaagacaaa aattactgtc atctgtcaga taagctcttc
     6601 atcatcactg tttgccctgt agccttcatc ttcagggaga cccggaagct ttgaagctgc
     6661 caccgcaaga gtatcacatc gttcattttc agggtcattg ctatgcccct ttacccagat
     6721 cagagtaacc ttgtgccgtc tgtaaacctc gaggaagcgt atccacagat ccttgttttt
     6781 ctttttggca aaccgtttca tttcccagtt gaagagccag ccgttgttga ctgcattgac
     6841 aagatattgt gaatcggtgt gcagtttcac tttcagcccg tcaagcctga gcgcctccag
     6901 cccggtgatg gctgccagaa gttccatgcg gttgttggtg gtgagcctga agcctcctga
     6961 gagctccttg cggagcgaac ccgagagaag caccacaccg tagcctcccg gaccgggatt
     7021 gccgctgcag gcgccgtcag tatagatagt aaccactggc cgactcattg cggtaggatg
     7081 atgcccgtgt tgttgatgaa cagtcttatg gcgatggaaa gaagaataat gccaaagaac
     7141 tttttcagta tgtgcagccc ggtgggcccc agcaaacgtt cgatccttga cgtgagcctg
     7201 agaacggtat aaatgatcac catgttcagt atcagggcaa taagtatgtt tatggttgca
     7261 tactcagccc tgagcgaaag gattgtggtt atggatcctg caccggcaat gagtggaaag
     7321 gcgatcggca caatggttcc tcctccgggc gaatcatgct tgaagaactc aacgccaagc
     7381 accatctcca taccgatcag gaagataatg aatgcacctg ctatggcgaa tgaacccaca
     7441 tcaataccga atatgccaag cagtttctca ccgaagataa ggaacgcaag gaaaatcaag
     7501 aatgaaacca gggttgcctt ccaggggtgg atgttaccgg tcttcgattt gatatcaagg
     7561 ataatcggga tcgatcctgg catgtcaatg attgcaaaca acaccatgaa gcaggctaat
     7621 atgtcaagga agttaatctg catccggaaa atttttgcaa aggtactcat ttagaagcaa
     7681 cagacaatag aggattctat gcagggggat gacccggcaa tccgcagctt ccggtaaata
     7741 acttactgat tgattgcaga ctgccggata ctgctgccgg ttaccggtgc cggcttccaa
     7801 ttgccggtct gctgactacc ggcttcctat tgccgtccac cgtctactga cttcctatta
     7861 ccatctgccg gcttcctatt accgtccgcc gtctgctaac ttcctattac cgtctgctga
     7921 cttccaattg ctgcctgccg tctgccgaca accgacttcc aattgccaac tttccctgtt
     7981 tattccctga tatccatttc atccagaaga tatcctgctc ctgaatagat atcaatgacc
     8041 cagagaacgt agcggatgtc aacgctgata catctctgga ggtcaggttc gaatgctatg
     8101 tttccggtca tggcctccca gttgccgtca aaggcaaggc cgataagctc gccgttgcca
     8161 ttaatgaccg ggctgccgga gttgccaccg gtgatgtcat tgttggtaat gaagcataca
     8221 ggcatgtagc catccggtga gccgtaacgg ccatactccc ttttttcgtt gaggtcaatg
     8281 agccgctgag gcagatcata ctcataatcg cccttcttgt atttctgcac aacaccgtcg
     8341 agggtcgtga tccagttgta atgaaccgca tcatacgggt agtagtcgag cactttaccg
     8401 taggtgagcc tcatggtgga gttggcatca gggtactgcg tcttgtcagg ctggtattcc
     8461 attgctccgg ctatgtacag acgctgtcct tttgagagat cagaactgaa ttcctgaaga
     8521 tcattcatta acgtcgagct catctcgctg attgatttgg caacaacaaa tgcaggatcc
     8581 ttcttaagaa ccttcaggct gggagcctcg agaaatgcat ccagtttctc cggcgagaca
     8641 aagattgact ttgcaaagat attatcaaca aaggcgtcaa tgttgccctt gaacttcgtg
     8701 tcgatcagcc tgtagaatga tggccagtac ttcggatcaa tgtcactcct gtagagcttg
     8761 aacattgtct ttgtggtttt ctgatcggta ggatagttat aatcatcgta gaaattttcg
     8821 gcaaatctct taagcctttc tgttgtactg tcgatttcct cagtatccct ttttgcaagg
     8881 gcatcttcga gcatggtcat ctggccggcg aagtttatca gttcggttga caatgcttct
     8941 ctgacatact gtgaaacgtt gtacttttct gcccttccct taacggcgtt ttcgatcagt
     9001 gaaagtgcat ctccgtattt tgccttgcgg gccggatcag catttaccca cttcatgtat
     9061 tcagcttcga aagcagcctt cttttctgcg gtgcgcaggc gggcgagacc ggcgttctgg
     9121 ccaattgaga acttccagta atttgatgag ccggagtatt ttgatgcata ctgaatattc
     9181 accttcgggt ctgccatcat atccttcatc catatctcct ggcgtgcacc acggatcttg
     9241 atcctgttgg cgttggtgat cttcattgcc tggtcaacct cccacgatgt catgtacctg
     9301 gtggtggttc cgggatagcc catcaccatt gcgaaatcgt tcttctgcag gttctttatt
     9361 gagacaggca ggtagtgctt cggtttgagc ggaacattgt ccttagagta ctgagccggt
     9421 tttccgtcag gacccatata taccctgaaa acgctgaagt cgcaggtatg gcgcggccat
     9481 acccagttat cagagtcgtg gccatatttg ccgattgagt tggggggtgt tcccactagc
     9541 ctgacgtcgg agaatacttc atacacaagc aggaaatact ggtttccgct gtagaatgat
     9601 gatacgcggg cgttgtagtg gtttcccttc gtggcttctg ctgtgatggc ggcgctctca
     9661 gccaggatgg cttcgcggcg tgccgcttcg gtcatctctg cattgacctt gccaagtacc
     9721 cttgaggtga catcctcaat gcgcaccagg aaggtgactg acaggccggg gttgggaagc
     9781 tcctcttcct ttgacatggc ccagaagccg ttattgagat agtcatgctc aacggtgctg
     9841 tggttctgta tctgaccata accacagtgg tggttggtga gcagcaggcc gtgaggagag
     9901 acgatctcag cggtacagcc gcctccgaag atgacgatgg cgtctttgat gcttgcctgg
     9961 ttgatgctgt aaatgtcttc cgctgtcagt ttgagtccca tcccggtcat agtgccgata
    10021 ttcagcttct cgatgagcgg cagcagccac atgccctcat cagccctggc ctgagaggct
    10081 gagaaggtta caaggataac aatgattgct ataaattttt tcatggttta tggtgttatt
    10141 gatttttgtt tggttccaaa gatatcgaaa taggtttcag ggaataatgt ctttaacatt
    10201 ttataagaat aggagtttcc cctgcgggtc agtcaggttg aatgagagac ggtggtacgg
    10261 ggtggggccg aattcagcaa tggctctgcg atgctccggt gtaggatatc ctttgttctt
    10321 acgccacccg tagcaggggt agagggcgtc aagctcctcc atatggtggt cacgctccgt
    10381 cttggccagc actgatgcag ccgctattga caggtatgtg gcgtcaccct ttattattgt
    10441 ctgatggggg atatttctat atgtgtagaa cctgttgccg tctatcagga gcaggagggg
    10501 gcggggttca agcccgtcga ttgccctgtg catggcggtg atggaggcgc gcagtatgtt
    10561 catttcatct atctcggtgt ttgagcatga ggcgatgcac catgtgatgg cagatgacat
    10621 gatttcggtg cgcagccgtt cgcgctggcg ggcggtcagt ctctttgagt catggagctc
    10681 tttgttacgg tatctcttgg gaagtattac tgctgcggcg tacacggggc ctgccaggca
    10741 tccccgcccc gcctcgtcgc aaccggcctc ccggatgccg ttgctgaaga atggtttaag
    10801 catcaggcgg ctcctccggt agctttgatg accgatttcc acatctcttc cgcagcctgt
    10861 tcccaggtgt acctgagtga gttggcatat ccctcgtcag ccaggcggcg gcgcagcgcg
    10921 ccatcgttga cgaggcgtgc catagcctcc gctatggcac ctgcgtcagc ggggctgacg
    10981 cagagcgccg cgccgccgct cacctcgggc agcgacgagc tgtctgacag aatgcacggc
    11041 gtgccgcaac gctgcgcctc caccacgggt atgccgaaac cctcgagcca cggcacgaag
    11101 gtgagcgcct ccgctgcccc gtaaagccgg cgcagcacat cacgcgtcac agaaccggtg
    11161 aagagaatat ccctgccata gggggagagc tccaggtgtt tgtccatctc acgggtcagg
    11221 taaagcctgc gcccggcaag caacagaaca tgttcaccgc caccgctctg cctgaaaatc
    11281 tcaaatgccc tgatcaccgc ctcaacattc ttcctcggag agaaattgct tacgaagaga
    11341 aaatatggcc tgccacccgt gtactgcctg cgtgtctcct ccgcctcgcc gggctgtggc
    11401 ggagaaaaaa cagcggcaac accgttcggc acgacatcta ttttgtcagg gctgatcccg
    11461 taggtggcag caatatcgcc tgccgagaaa tgtgagacag tggcaacacg tgctcccttc
    11521 gccgcgaaga gagggaaacg tctgcggtaa tacctgcact caccgccggg tatgtctccg
    11581 ggccggtgct catggttgag gtcatgtatc accggtatac aaggcacttc cgttctcaac
    11641 ggaatcatcc cgtcaggcgc gatgaacaga tccgccttta tccgtttcag taccggggga
    11701 agcaggtatt cgtgccatgc atgccacagc agaggatgca ctgtccgcag cggaatggtc
    11761 acatattcaa cattcctgcc ttcaaccgga agaattccgg atgccctggc atctgcagcc
    11821 ggcgcctgct catcctgccg gttgccttca gccgtcgcct gctcatgctg ccggttgcct
    11881 tcagccggca acctgccgta cctcctgtca ccgatcagca caaaccggtg ttcggggtgg
    11941 ttgcggacca tcccgctgac aacctcaagg gtgaaccacc cgatgccgtc acgtggcact
    12001 gccctgagcg tacgcgcatt aaccgcaata atcattttcc gttgctgctt gtaaaataca
    12061 aatgtggcaa ttaatttatt aaattggcat tgattcttct atccactgca gactgatttg
    12121 aggcgaaagt tccttatcaa tatcaccttc ctcctgacgg tgagcatcct ggtcaaggct
    12181 ttctgggtgc tgggcataga ccgcaccgtc cagaacgtgg ttggcgagac cgcctacgga
    12241 aactattttt cactcttcag cttctcggtg ctattcaccc tcctgcttga catgggactc
    12301 tcgggattca acaaccgggc tgtatcggct gatcctgcaa gggtaaggat ctacttcggc
    12361 aatgtgctgg taataagact ctttcttaca gcactatact tcctggtcac catcagcgtt
    12421 gcctatgccc tgggttaccg gggagagcag ataacaatac tgctggttct catgctgaac
    12481 caggtgatgg catcaatgat catctggctg cggtcgagca taagcggcat gcagtacctc
    12541 tttattgaca gcctcctttc cgttgccgac aggctggtaa tgattatcat ctgctcggtg
    12601 ctcctgtggg gcggagtcac cgccgggggg ttcaggattg aatggtttgt atgggcgcag
    12661 acggctgcct atttcacggt catgtgcact gcattcataa tagtggtgta caaggggaag
    12721 gtggcggcag tgaagcctga tatctcagtt ctgaagagta tcattatgac cgggctgccc
    12781 tattcggttg tggtttttgc aatgactctc tactggcgga ttgattcggt gatgatcgaa
    12841 cggctgcttc ctgacggggc cacccgggcc ggtaactatg cccaggcctt caggctcttt
    12901 gatgcgctgg caatgattcc tgtgcttttc ggcgggatac tgcttcccat aatgacacgc
    12961 gggctctcat caggcagtga tatcaggcct ctggcaagga tggcctgccg gatgctcatg
    13021 gtcccgctgg ggataggtgc tgtgaccctc gccacgtttc cggcagagat gcttgacatg
    13081 ctttatactt caccggccgg tgatgccgtg accgctttca tgatactgat gtttgccctc
    13141 gtgcctgtag gagtgatata catatacagc acaatgctta ctgcttcatg gaagcttaaa
    13201 atgcttggtg taataaccgt atcaggcatg atcatcagcc tttcgctaaa ccggattctc
    13261 atcccggagc tgggcccggc aggagcagcc tctgctgcac tggtcaccca gtcactggtt
    13321 gccgtggcct gcatgctgac cgtgagaagg acgatgtccg gcattgccgg gaacggaagg
    13381 attctgctgt tcctgctgat gcttggactc accttcttta cgggatggct cctgcgcaga
    13441 actggtatcc cgtggatagc tgcagctgcc ctggagcttg caacgggcat cctcctggct
    13501 cttttgttca ggttcattgt gcctctctcc ggcatcagac tgattaccgg gaggttcgga
    13561 acagagtgaa gagggtggcg ccatgcgctt atacgagacg gtagcccgtg acaacaacgg
    13621 cagggagccg gcaaacacgc cggcagagga gcggttccca atcgcgccag actcatccac
    13681 cggcctgacc gatcacccgg tggactttaa gctcagcggg ctatctctca atgctttttt
    13741 tatctatctt tgcgcaaaat ttcagatgac atgataacca atgaccagat acaggacctc
    13801 gttgaacgcc gggatgcgtt aaggaggtat ctttgatgtc gatgccagac taaacgaaat
    13861 cagggagaag gaagagatca cccgttcggc cggattctgg aatgacccta aaaaagcaga
    13921 ggagctcctg aaggagatat cctctgtaaa aagctggacc acagcctttg ccacagttga
    13981 tactgccgtt gaggacatgc ttgtttttta cgacttcttc agggagggtg aggtgactga
    14041 tgaggagatg ggtcagcaat acgataaaac tcttgcagaa gtagaaaaac ttgagctccg
    14101 caacatgctg cgccgtgagg aggaccgcct tggcgccctt ctcaagatca atgcgggcgc
    14161 cggcggcaca gagagcaatg actgggcccg tatgctcatg aggatgtacc tcatgtgggg
    14221 agagaagaac ggatacaaaa taaccgaggc atactaccag gcaggcgatg aggccggaat
    14281 aaagacggta accctcgagt tcgagggcga aaacgcctac ggctacctca agagcgagaa
    14341 cggcgtacac cgcctggtcc gcatatcacc cttcaacgcc cagggcaaac ggcagacgac
    14401 attcgcgtca gtcttcgtgg ctccgctggt tgatgatgac atcgttgtgg agataaaccc
    14461 gtctgacatt acctgggaga ccttccggtc atcgggcgcc ggcggccaga acgtcaacaa
    14521 ggtggagacc gccgtccgcc tccaccatgc ccccacaggc atcgtcatcg agaaccagga
    14581 gtcgcgctcc cagggacaga acaaggagaa tgcactgcgc ctcctcaggt cacatctgta
    14641 tgagatggaa ctcagaaaaa aaatggagga acaggcaaag attgagggcg caaagaaaaa
    14701 gatcgagtgg ggttcacaga tcagatcata tacactgcac cctttcaagc tggtaaagga
    14761 cctgcgtacc ggttgcgaga ctggcaatgc ccaggctgtg ctcgacggcg agatagacga
    14821 attcatcaag gcatacctga tggagtacgg cggaaaggag tgaacagtca tgataaaaag
    14881 catggtttaa taatcttaca acacgttaat ttacaaccct cagcaacata aatcctgatg
    14941 gataagaaaa agaagctatt tgaacaattc ccgcctgtat ccacaacgga gtggatggag
    15001 aaggtcactg ctgatctgaa aggcgcggac tttacaagga gacttgtctg gaaaacacgt
    15061 gacggccttt ctgtcatgcc tttctacagg gaagaggatc ttgacaaact gcctcacagt
    15121 aaccttctgc ccggcgactt tccctttgta agaggagtgg aggtcatggg caacaactgg
    15181 ctcattcgcc aggatatcac ggtgactgat tataaggctg caaatgccaa agcccttgac
    15241 atactgatga gaggcatcac ctctctgggc tttgtcattg aagattctca gtcggtcact
    15301 cctgatactg tggcagagtt gctgaaggat attcatcctg agagcattga gatcaacttt
    15361 tctgttgccg gcggtgcgaa ggagctcgtt gccgcgctta agggatactt aagctcatcc
    15421 ggcgctgccc tgaagcaggt gaagataacc gtcccggctg atccgctggg cagactggct
    15481 gccaggggca ggttgtgcgt gcccgttgat cagggtctcg attacctggc agacctcgtc
    15541 agtgatgcag cggagatacc aggcataagg tgcgtggagc cttcaggaac actcttcagc
    15601 aacgccggcg ccggtcccgt ggctgaactg gcatatacac tatctctcgg aaacgaatat
    15661 atggcagcgc tgaccggcag gggcactgac cctgacactg cagccaaagc cttcaggttc
    15721 acattcggca tcggtcctga cttcttcccg gagatagcca agctgagggc agccaggatg
    15781 ctctgggcag agatagtcag tgcctggggc acgaaggagt ccggatcagc cctgatgcat
    15841 atacactcgg tcaccggcag gtggaacaag accctttatg acccgtatgt caacatgctg
    15901 aggacgcaga ccgaggctat gtctgctgtt ctgggaggcg ccgagtcagt aacggtggaa
    15961 ccattcgata cggtgttccg gagcgcaaac gacttttcgg agaggatcgc ccgcaaccag
    16021 cagcttctgc tgatggaaga ggcgcatctc gggaagattg ccgaccccgg agccggatca
    16081 tattatatag aggaactgac ctcaatgata gtcagtgagg cctggaaatt attccttgag
    16141 attgaagagg agggaggatt ccttgcagcc cttcgcaaag ggatgataca gcagcgcatc
    16201 agtgaggctg cagcaaagcg caaggctgac atggcaagaa gaaaggaagt tcttctggga
    16261 acaaaccagt accctgattt cacggaggat aaggctccgg agcatgatcc cgggaaactc
    16321 gttactgaac agacgccctc ttcggaggag gtcatgcctg ttattcctgc ccgtggcgca
    16381 gatgagtttg agaagctgag gcttgcaacg gagaagtccg gccgcaggcc gctggttttc
    16441 atgctcacca ttggcaatct ggccatgcgc agggcaaggg ctcagttctc ggccaacttt
    16501 ttcgcagtgg caggttatga ggtaagggac aacaatggct ttgcaagcgt tgccgaaggt
    16561 gtcactgcag ccctggaggc aaaggcagac ataattgtta tctgcagttc tgacgatgag
    16621 tacgccacac tggcgaaaga ggccttcact gcaaccggca gaaaggctct cttcgtggtg
    16681 gccggaaacc cgccatgcat ggaagagctc agggccgaag gagtggagca cttcatcagc
    16741 atcaaatcaa atgtgcttga gacactgcaa atgttcaaca ggatactggg aataaactga
    16801 cgggagatga gcaaaagacc aaaatttaat gatattgacg ttctgaaggt gaccgtgcct
    16861 tcaagagaca aacaaaagtg ggagaaagaa aacggtatca gcccgcagtg ggagacgcct
    16921 gagagaataa tcctgaagag cgtttattcc cgtgaagatc tgaagggcat ggagcacctg
    16981 cactatgctg ccggtatacc accctatctc cgcggaccct attcggccat gtacgccatg
    17041 aggccgtgga ccatcaggca gtatgccgga ttctccaccg ctgaagagtc gaacgccttc
    17101 taccggagga accttgctgc aggccagaag ggactttcgg ttgcctttga ccttgcaaca
    17161 catcgcggat acgactctga tcatgagcgc gttgttggtg atgtggggaa agccggtgtg
    17221 gcagttgatt cggtacagga tatgaaaatc ctgtttgacc agattcccct cagggagatg
    17281 tcggtatcaa tgacaatgaa cggagcggtg ctgcccatta tggcattcta catagtggca
    17341 gggctggagc agggggcaaa gctgagtgag ttgcagggca ccatccagaa tgatatcctg
    17401 aaggagttca tggtccgcaa cacctacatc tatccgcctg cattctcgat gcggatcatt
    17461 gccgacatat tcaaatatgc ctccgggaac atgccgaagt tcaacagcat ctctatctcg
    17521 gggtatcaca tgcaggaggc gggcgccacg gcggatatag agctggccta cacccttgcc
    17581 gacgggcttg agtaccttcg caccggggtg aatgccggca tgaacattga cgacttcgca
    17641 ccgcggcttt catttttctg gggcagcgga atgaacttct tcatggagat agccaagatg
    17701 agggctgccc gaatgctctg ggcaaagatc gtcagccagt tcaacccgaa aaatccgaag
    17761 tcactggcgc tgcgcaccca tacccagaca tcgggatgga gccttaccga acaggatccg
    17821 ttcaacaacg tgggccgcac ctgcatagaa gccatggctg cagcgctggg ccatacccag
    17881 agcctgcaca ccaacgccct tgacgaggcc atcgccctgc ctaccgactt ctcggcaagg
    17941 atagcacgca acacccagat ttacatacag gaagagaccg gcatctgcaa ggcggttgac
    18001 ccatgggcag gctcgtatta tgttgaaaca cttacccatg agatagccca ccgggcatgg
    18061 gaactgatag aggagattga aagtcttggt ggcatggcaa aagccatcga gtcaggcata
    18121 cccaagatga ggatcgagga ggcagctgcc agggcacagg caaggataga ttcgggagcg
    18181 cagaccatag tcggcctcaa caggtacagg ctggacaagg aagatccgct tgagatactt
    18241 gaggttgata acagcgcagt gcgcgaagct cagttgaaaa gacttgccga agtgaaggcc
    18301 ggacgcaacg aagaggattg ccgcagggct ctcgatgcca tcacccgtgc agcagaaacc
    18361 ggtgagggca atctgcttga actggccgtt gatgctgcat caaagagagc aacactgggt
    18421 gaaatatcat acgcatgtga aaaaatagca gggaggtaca aggctgtgat tagaacaatt
    18481 tcaggggttt attcatcaga gtacaaatca gattctgatt tcgaacaggc aagggccatg
    18541 gcagctgagt ttgccacccg ggcgggaaga caaccccgca taatggtagc caagatgggg
    18601 caggacggtc atgaccgcgg agccaaggtg atatcaaccg gatatgccga tataggattt
    18661 gacgtcgaca tcggaccact cttccagacc ccgtcagaag ctgcacgaca ggcagttgag
    18721 aacgatgtac acctgctcgg ggtctcaagc cttgccgcag gtcacaagac tcttattccg
    18781 caggtcatca gcgagcttaa gaaacttggg cgggaggata tccttgtgat tgccggtggt
    18841 gtcattccgc cgcaggacta ccagttcctt tatgatgccg gtgtggcagc aatctttggc
    18901 ccgggcacat cagtggcaaa aacagcaaaa gagatattaa ccttgttgtt gcaaacggta
    18961 tgatgagaac agctttcgcc ttgctgtcag tggtggccat cgctctcctt gccggctgca
    19021 gcgccggccg gaagaatgtg gcagtagccg gggaaccctc tgctgcagta gccgcagaac
    19081 aacaacagcc cgaggcacag gttccggatg ttgctccctt cggcaatgcc gcccttgcag
    19141 ggaaaccgga aggtgatgag ggagccgcac acgtatggga tttccgcgat gccaccacat
    19201 ggctgctggg agaattctta cccgggatgc tgaacaatcc tccgcattca gaatggtatc
    19261 atgaaggcta cgccggttac cagcctgacc aggaagtaat ggcggaatta atggaaatcg
    19321 aaaaggatga cctgacaatc acaatcgtaa taggaacatg gtgccccgat agcaggagag
    19381 aggttccgcg ctttatgaaa attgctgacc tgtggggatt tccgcatgac agaatcaaat
    19441 tcattggggt tgacataaac aaggtggcac cacttgaaga ttactttcag ctggctattg
    19501 aaagagtacc gacatttatt ttttataaaa aaaatattga aaagggacgc atcattgaag
    19561 ttcctgagac gtctttagaa caggatacaa gaaatatcct caaaggaaat taggaacaat
    19621 taatacatga accaatggaa gaagaaaaat ccgccggaca gtcgtcagga acaggaatgg
    19681 gtgttggcgc ccttgtaacc gccataatag ctttcctgct ggcagttatt ccatgcgtag
    19741 gcctggttgc tgtaatcccg gcagtgatag ccattgtcct tgccataata gggctctcaa
    19801 gaccccaggt taaccagggg atgctcgttg gcggacttgt agtaggcatt atcgccctga
    19861 tgatatcagc ctcacagatc tttgtcctcg gcaagattgc agaaaaatcg ggcagctggg
    19921 caacgaatat cgagaaggtt ataaggaatg ttgccgatga catcgaggat gagttcggtg
    19981 accaggaggt gacaatcaag atcaacaagg atgatgaaac cgtggagata aagtcttccg
    20041 gaaagagggg tgatcttgag gagaggctgg aggagcttga agggtctgtt gacaccctga
    20101 agagccgctc tgacaccaca aagggagagt ggtgaggcca aaccgggctg caaggccgta
    20161 tctgatactt aatcttatac ttgccggagt aatcctgttg atcatgggtt actccctttt
    20221 ttattcgcct gaggaggatg agtacccggt cccatgcatt catgagaaac tgaccggtga
    20281 gccatgcccc tcatgcggtc tgtcgcatgc attttcactt atcgtccgcg gcagggttga
    20341 tgaggcgctg gagtggaaca gtcagtcgct cagggtgttc atcttttttg cgcttcagct
    20401 tgtgatgcgc tccggtcttg cagtgacggc catgtgggtg aagcggaatc tcagggtggt
    20461 tgccgttact gatgctgtca tatccggcgt gatgaccctg gctgcctttt ggccgtttat
    20521 caggatgctg tggatcagcc tgttctgaga tatgaggaat ctctcccgcc cggcagtacc
    20581 ccactcactc agcatcatct ccctcacaca gaagtgagtt gatagcatca tagacaaggt
    20641 ggctctcaaa gcctttcccc agtgcatatc tgagaagctt tcccttgaga tcaagcctgt
    20701 tttttgcctt tatcctcctg cgctgatcgc tcaggatttt ttgcagcaat tgcaggtatt
    20761 catcttcatc aattgcttcc atccctgatg ctatggcttc cgggttgatc tttttctgtt
    20821 tgagtgccat gctgatcttt acgcgccccc acaggctgtg cctgaaatgg tcaagcacga
    20881 atgccctgct gtagcgttgt tcatcaatga acttttcctt tatcagcctc ccgatgatac
    20941 gggtgcgtgt ttcagcgctg tcagcacccc agcgttcaag cagcgcctcg atatcactga
    21001 tacagtattc ccttgccgag caggctctca ttgccctttg cagcaactca tccatctctt
    21061 tcttttcagc catatttctc agctttgata aatctctctt ttccccttat atcccttata
    21121 atccgcacct cactgtagct tttcggtgac agcagtgcag cggtctctcc tgcaagattc
    21181 tcattcacct caagccacag ggttcctccg ggcaggagac gtgtttcagc cacggctgct
    21241 atggcgcggt agtaaagcaa cgggttgtca tcaggcacaa acagggcctc atggggctca
    21301 tgatcaagca cgttgcggtg catcatcgcc ttctcgcgca tggtgatata tggcggattg
    21361 ctgacgatga tactgctcag gggtatctgc agggggtcat cattcaggat gtcagatttt
    21421 atcagtgtca cggctgctcc gttgctgcta atgtttcgcc gtgccatctc cagtgctgcg
    21481 cctgacagct ctgttgccgc aacagttgca cgggggaatg ccatggccat ggcaattgcc
    21541 atgcaccctg atcctgtgca gatgtcggtg acggtgccgg tgaagccgct gttttcactt
    21601 atgatcatca tggtcatctc ctctgtctcg ggtcttggta tgagaacacc cggcgccacc
    21661 tcaatcctgt gtccgcaaaa ctccgtataa ccaaggatat actgcagcgg ttcgccggct
    21721 gcggcgcgtg ccacggcatt ctctatacgg gcggcggcac cagcctccgg gacacgctct
    21781 ccaaaagcca tctgcgccgc cctgcccatc ccggtatact cacctatgat ggctgacgcc
    21841 agcgccgcag cctcctccgc aggccatccc gccttaagca attcagtgat acgaccacgg
    21901 atttcgctta ttgtttgtac ctttccgtcc atgattcaaa gataggaaat tgagctcatg
    21961 ccccgacctg atgacctgat atggatgaga cgcgccctgc agctggcagc aatggctgag
    22021 ggatgcacct cacccaaccc catggtgggc gcggtggtag tgcataacgg cgtcattatc
    22081 ggagaagggt atcatctgaa agctggcacc ccccatgcgg aggtgcatgc catcaatgcc
    22141 gtcagcgatc gcactctgct gccatcctca accatttatg tcaccctcga gccatgctca
    22201 caccagggac gcactccccc atgcagtgac ctgatagtca gcagcggcat acgccgggtg
    22261 gttatcggcg ccgcagacac caacccgctg gtggcaggca gggggattgc ccgcctgagg
    22321 caggcaggag tggaggtgat aaccggtgtg gcggaggagg agtgccgccg tctcaacagg
    22381 cgcttcttca cctggcatga aaaaaagagg ccatacgtga tactcaagtg ggcccgcagt
    22441 gcagacggtt tcatcgaccg gtgcaggaag gaatctgacc cgccagggcc aacctggatc
    22501 acagggatgg cagagcgcgt tctcgtccac cgctggcgcg ccatggaaga tgccattctc
    22561 gctggcggag gaaccatacg tgctgacaat ccatcgctga ccgtcaggta ctggagcggc
    22621 cggaatccgg tacggatcat cgtcagccgc agcggaaacc ttgatcctga ttcgtcactc
    22681 tttgacggga gagctgagac ggttctctat acctgcaacg gacaggcgca attccgtaac
    22741 accagaaccg tcacactgac gggcgctgaa ccgtttatcg gcgaggtgct ggcaaatctg
    22801 tggcaggagg gcatacagtc actctttgtt gaaggcggag catacatcat aggcagtttc
    22861 attgaatcgg ggcagtggga tgaagcacgg cgattcaccg gcactgcaag cttcggtgac
    22921 ggggtgcccg atccgttccc cggattcact cccggggaaa cagtgctttt tgacaaaagt
    22981 attctggaat tcgcgtatca tttttaggag aattccgtat aattaatgtt aataactgct
    23041 tttatattag taaatattaa ttcgtatttt tgagacccga ccgggaaagg acctaaagtt
    23101 atgcagcaag cattcattgt tcatgttaca gaagccgtta ccggaaacaa ccggcagaaa
    23161 tccggtttct gcatgataac cagcctgaag gatgaagacg aaccattctt ctttgtaaat
    23221 aaataatctg atgcgaggac gaggcagaaa ctccggcaga tctattatta tcctggcatt
    23281 actgcttctg tctcatgtta tacttattcc gcggctatcc ggtcagacat tctattttga
    23341 acagtatggc tccaaggagg ggctgagcgc ctccaaggtt tatacaatca tacaggatgc
    23401 caacgaattc atctggcttg gtaccggtag cggcctcacc cgctttgatg gcctgcactt
    23461 tgaaaacttc tccacttctg acagccttgc tcccggaggt gtaaaaagca tctgcgagga
    23521 cagtcgcggc agactctggc tggggcatat cggagggggt gtctcaataa tagaggacaa
    23581 cataatcaga cgagtctgct tttccccttc cattttcatc ggggttgatt ctgcccgtat
    23641 cagagacccg ctgaagccca taaacagcct gacgggagat attaccggaa tcactgaata
    23701 tggtggttca atctgggtct caacctcccg cagcggagcg ttgaagcttg acatgcccgg
    23761 gcatggtgtc agggtgatga ccggccagca gtacaccggc cgccagggac tcagcaatga
    23821 tgccttcggc ctttatgccg atcaccgcga aaacctctac tgcatcaccg acgtgggcat
    23881 caggaagtat gaccatgcaa gtgacaggtt cattcctttc catccggaag gacttacacg
    23941 ctacttcctg acaacaacca tcttcgagga ctcccgcggc aacatgtggt tcggcacctt
    24001 caacggcggg ctttacaggc aggacagtga gacgggcaag atgggactgt ttgatacccg
    24061 caacggcctc tggggcaact ttgtctcata catccttgaa gactatcgcg gcaatatctg
    24121 ggtagcctca atggaagact gggagagcag gggagggata acggtatttt caccggaggg
    24181 aacagttgtc tacaacaaca gcaacggtct gcaggcacag catatcctct gcatgattga
    24241 ggacaaggag aagaatatcc tgatagctga ccgcgatgcc ggccttttca tttacaaggg
    24301 tgatcacttc gcctcgttca tggcgccgcg gttcatgccg tcaaatgatg tcttcaccct
    24361 cgcccaggat catacaggca gatactggtt cggcaccggc agtggcctct cgctctacaa
    24421 ccccgccctt aaagaggatg accgtgtcag gctcagggga ttcccgccgt cgcttgagga
    24481 gacacagata agcatcatca ggcctgacaa ggaggggaac gtatggattg ccacggcagg
    24541 cgatgaggtc taccgctatg acactgatga taagaatttc cggtatgacc ttgaccttaa
    24601 cgccagcctc cgcccgctga gaatcactgc catgaccact gaccagagaa acaacctctg
    24661 gatcggcact gacggggcac tcatcatgtg ggatccgcag ctcaagaaaa gcacgaaata
    24721 tacccggagc gacggcctga cgggaaacaa tatcacagcc ctcttctgtg acagccgggg
    24781 ctcactctgg ataggcacgg aggaacaaaa aggccttgcc aggctcaacc ccgggaacgg
    24841 cactttcatc accctgacct tcagatcaga tgtgcatccg acagccatca ccgagactcc
    24901 tgacggaatg atctgggtgg ggacaattga cggactctac ggcctgagca atgacacggt
    24961 gaaaaagaaa ctcaccgagg ctgaagggct gctgatgaat tcaatcaacc ttcttcagcc
    25021 tgacaggtcc ggcaacctgt tcatcggcac caacctgggg ctgaacaggt atgacagcaa
    25081 taccggccgg atatcatcat tcaccgaaaa gagcggattc accggaattg agacaaggcc
    25141 caatgcttca tttgccgatg acatgggcga tctctggttt ggcacttcca acggggccat
    25201 gaggctctct ccctccatgt tacctccggt ggtgacagaa ccggcaacac atatatggag
    25261 aaccctggtc aactatgagg tctatcccat gcatgacgga ataaggctcc tccacaccca
    25321 gaacaggata atttttgact tcctgagcat ctgccttgtt aatcccggcg ccgtgagata
    25381 ccagttcatg cttgagggcg ctgacaagga gtggatcacc actgaccaga acagggcggt
    25441 ctactcgtca ctcagacccg gcaagtatat cttcctggtg aaagcttcaa acaattacgg
    25501 tttctggaac gaggtgccgg caaaactgtc attctccatc agggggccgg tatatctgac
    25561 atcctggttc atcatactct gcatagccgt ggcagtggct ggtgtatatc tctatataag
    25621 cctccgcgag agaaaactgc gccgggagaa ggagcatctt gagcagaagg ttaaggagcg
    25681 cactgccgag gttgtcagca agagtcatga gctggaggag aagaacaggg acaccaccgc
    25741 cagcatcagg tatgcagaga ggattcaggc tgccatgctg cccccggaga attacttcaa
    25801 tgacaccttc gtgctgttca tgccgagaga cattgtcagc ggcgactttt actggatgta
    25861 tgacaccggc tacaagcagt atattgctgc tgtagactgt accggtcacg gcgttcccgg
    25921 cgccttcatg tcaataatag gccacaactc cctcaacaag attgtcaggg agagtggtat
    25981 tgaaaaaccc tctgacatac ttgaccacct gaacatcgag gtggtgcatg ccctgctgca
    26041 gcgcagcgaa aaggctgtca gggacgggat ggatatctcg gttatctcat atgatattct
    26101 cgagcatacc gttgagtatg ccggggcgta ccagcccctc tatcacgtcc gtgacggcaa
    26161 ggttacggta tacaggggtg accgcttccc gataggaatg atcgacggcc aggccaggaa
    26221 gagttttacc aacaacctgg tggacgtaaa gtccggagac atgctctata tcttcacaga
    26281 cggctttgcc gaccagtttg gcggggagca cgagaaaaag ttcaagaccc tgaaaatcag
    26341 gtcgctgctc tctgaaatct atatgttttc cgttgaggag cagaagcgcc gtcttgaaaa
    26401 agccatcagg gagtggatgg gtgaactggc acaggtggat gatatcctgt ttataggtac
    26461 cagggtacct taaatccggg tcagccctgc agacatatct gatggctcat gtttccgggt
    26521 cagcctttca gacatatctg atgattcagg ttcagatcct ggcaatcgtc ttcgggtctg
    26581 cataccaccc ggtgtaattg acaatagccg gcataacctc ttcggcagtg gtgactatct
    26641 gccagatatc cttgtgttcc agcctcatga agtggcctct gaccatgtgg tcaaagaact
    26701 cgatgaggtg gtcatagaag ccgttgacat taatgattat aacaggttta ttgaactggc
    26761 cgagctgctt cagtgttatc acctcagtga gttcttcaag tgtgcccacg cctcccggaa
    26821 gcgccacggc agcatcggac atggcaaaga tggtcttctt cctctctgac atgtcagggg
    26881 ttacaatcat atcactgacg ccgctgtgcc cccatccttc atccatcata aaacccggga
    26941 tgacaccggt gatggaaccg ccattagcca tgagcgaatc ggcaaatatt cccatcaacc
    27001 ctatgccgcc accaccgaac acggcatgta cccggtgcct ggcgaatgcc atgcccagtt
    27061 ctttggcagc cttcatgtag gcagcgtcaa tccggctgct ggaggctgca aatacacata
    27121 ccttcattat ggtatcagag caggggagtg agtttggcct cgaaggctga gcgggcagct
    27181 gcgcccacct gcttgtcaac cacctcgccg ttcttgaaat aaagcactgt cgggatattc
    27241 ctgatcgtaa agcggcgggc aattcccggt gaactttcga tatcacattt aacgaccagg
    27301 gccctttccc tgtaagcatc tgccaactgt tcgatgtatg gcgctatcat tctgcagggt
    27361 ccgcaccatt cagcccagaa atcgacaata accggtttgt ctgacttcaa tacaacctca
    27421 tcaaaattgc tgtcgttaac ttgttgtgtc ataatattat acgtttctta aattgctttt
    27481 tttgcgttcc aaagatacct aatttacttt aaaccccagt tcaggattat cttcaatcca
    27541 tccgataagt tcatcggtga ttcctgcctt tatgcttcgc gagaagagcg ggatggtaat
    27601 ccgctcgtca tggtcaatca gcaggaattt cagggatttg tttcccgggt tttccaagat
    27661 gactgacttc aggtttttaa tgaattcggg attaaccagt tccggtttca gttttattgt
    27721 aagagaggtt atcaggtctt ccctgactgc tgtcagaagg tgtatctcct ttatcctgaa
    27781 ttcgagctcc tctggtttgt aatgtcttcc ctgcaccctt cctttgacaa ggagctggta
    27841 gtcattcctg aagtagttgc tgaaggcaac ataatccttg tcgaacagca ggaaggagaa
    27901 tgagtcggta taatcctgaa gtgtgaaccc accgaagggt tttccgtttt tggtcactcc
    27961 gcttcgggta tcgctgacaa ttcctgcaac gcagacgtca cggtttgcga actcgctcag
    28021 gttctgaagg tctgccaggc ttgcattgca gaatgtattg atttccagct taaagtcgtc
    28081 aagagggtgt gatgagaggt agataccgat aacctccttc tccttgttca gtttttcaag
    28141 cttagaccag tcggggcatg gtgccggttc ggggcgcaca aggtcaaatc ctccggtatc
    28201 accgaagaga gactgttgag tgctgtttct gatgttcttg acgttgttgc cgtagcggat
    28261 gaggctttcg aggaagcttg agcccttgcc gtctgcggcg aagtaccgtg ctctggtcat
    28321 atccggcagc ccgtcgaggg cgcctgatac caccagggct tcaagcattt tcttgttgac
    28381 ggtattcagg ttcacccttt ctgcaagatc atatatgtct ttgtatggcc cgttcttttc
    28441 ccgttcctct atcaggtgaa gcactgcatt ctcacccatt cccttgatgg cgcccagccc
    28501 gaacctgatg ttgcctttac tgttgacggt aaactgcagg aggctttcgt tgacgtcagg
    28561 ccccagcact tctattccca tgcgcctggt ctcctccatc atgacggtga tcttctttat
    28621 gtcattgatg ttccggctca gtactgccgc catgaactca gccgggtagt ttgccttgag
    28681 gtatcctgtc tggtaggcta tcagcgcata acaggtggag tgtgacttgt tgaaggcata
    28741 ttgggcgaat gactcccagt cagaccatat tctgtttatc gtgttctggt catagccgtt
    28801 cgcctggcaa cccttgatga actgctcctt cagcatgttc atgatgtcga gttttttctt
    28861 gcttattgct ttgcgcagcg agtcggcctc gcctttggta aagccgccaa gttcctgtga
    28921 gagaagcatc acctgctcct ggaagacagt gatgccgtag gtctccttca gatacttctc
    28981 cattgccggc accacgtacg agatcttctc ttgaccgtgt ttacgcctga taaactgagg
    29041 tatgtagtcc atcgggccgg ggcggtagag agcgttcatg gcgatgagat cgccaaactg
    29101 gtttggtttc agctcgcgga gatagcgttt catgccgaca gattcaaact gaaacacacc
    29161 tgttgtctcg ccgttgctaa agagttcata ggtagcccgg tcgtcaagtt ccagggtctg
    29221 gagatcaatg gttatgccct ttgatttttt tatattttca acggcatcct tcattatcga
    29281 aagtgttttc agccccagaa agtccatttt cagcaggccg acaggctcaa cgtgattgcc
    29341 ctcatactgg gtcacataga ggtctgtatc ctttgccgtg cagatgggta tatgttcaga
    29401 caggctattc ttgccaatga ttataccgca ggcgtgcacg ccggtctgcc tgatgcttcc
    29461 ctcgagcacc ccggcatatt tcagtgtctg tgccaccagc ggatcctttg aatccttctc
    29521 tttaaggagt tcaggcacct cttcgtaggc tgccttcagt gttaccccgg gcttgtcggg
    29581 caccatcttt gtcagtctta ttgcttccgg cagtggcagt ttctgaaccc gtgcaacatc
    29641 cctgatagcc atctttgcgg ccatggtgcc gaatgtgatt atatgtgcca cacggtcatg
    29701 accatatttt tcaaccacgt acctgagcac cttttcacgt ccgtcttcgt cgaagtcgat
    29761 gtctatgtca ggcattgaga tgcggtcagg gtttagaaat ctctcgaaga ggaggttgta
    29821 ttttatcggg tcaatgtctg taatccggag ggaatatgct acagcgcttc ctgcggcgga
    29881 gcctctgccc ggccccaccc atacgcccat ctcgcgggca gccctcagaa aatcctgcac
    29941 aatcaggaag tatccgggga acttcatatt ttttatta
//
LOCUS       DAOPBK010000005        28209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_1833_length_28209_cov_5.499219, whole genome shotgun sequence.
ACCESSION   DAOPBK010000005 DAOPBK010000000
VERSION     DAOPBK010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28209
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1833_length_28209_cov_5.499219"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..550
                     /locus_tag="PLA17_00785"
     CDS             239..550
                     /locus_tag="PLA17_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HPK83977.1"
                     /translation="MGKGDRKSKRGKIIKGSFGVRRRRKVRKSFVAPSPKETVKATPA
                     ITPEAEEAKPARTARPATVAKAATKPAAKKSEKETGTTAEKPAKAATKTKKPAAKKEE
                     E"
     gene            complement(559..2295)
                     /locus_tag="PLA17_00790"
     CDS             complement(559..2295)
                     /locus_tag="PLA17_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPK83978.1"
                     /translation="MVIEDFLARQLREAGVRRFFGVPGGPSIPYLEAFRKAGIEFILA
                     SHEASAAVMADVTARLTGVTGVCHATFGPGAVNLSSGVGGALLDRSPLIALTTEMSEA
                     WLGRTSQMNIDHQVLFRPLTKETFRLNADNAREIISRALELANEEYPGPVHMGLPSDL
                     AGSVAGPYTGASGSAGISGIDSTAGVSGAGVETPWHASAGGGSGSGAGISRHTSVPGA
                     SGARVRISGHASTGEDNRARAGDLIASSRRPLIAVGLTAMRTVAGRSLVRFLEEHKVP
                     VVVTPMAKGVITHDHQSFAGVLFHALSDRLAGLIRAADLVIGLGYDPVEYNYESWLPE
                     VPLVHFDTKPSDLRISGAIEVVSAPEEWFMVLSSLGSSDEMMALAARTRDEIRTSLKR
                     AGSGLNPVTALSLLRELLPSDTTVTADVGSHLHLAGQMWDLQRGKLIMTNGWSSMGFG
                     LPAAVAAALAGGGGPVVCVTGDGGFAMSAGEIMTARRLGLKIIVVVFSDGELNLIKVK
                     QSWKSLSPYGIHVHDGSLFGADRFLGVDVQYVTDLSGMRSALKRALASGGCEIIEAAI
                     NPSVYDDLIVKS"
     gene            2431..4689
                     /locus_tag="PLA17_00795"
     CDS             2431..4689
                     /locus_tag="PLA17_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPK83979.1"
                     /translation="MKRTFICVFLSFLFAATLSHQGSAQGTEKDYERANNIDRQLAGK
                     VWHSDIRPVWVGKTNMLVFEDRTPEGKDYILADILKKTSKKAFDAARLADALGKITRD
                     TVRAADLPIRNLVMADDLKSVTFEYSGAMYKCDLKKYTVTRERETRRPSAWGGEERWP
                     WGFRNELIGRPDTSPDKSMTAFIRDYNLWIRVLKDKKEYQLSHDGGIGKYYSASVYWS
                     PDSKKIMANLVVPAEKHMISYVESSPEDQIQPKYYTREYPKPGDAVPQFFPQIFDVAG
                     KKHIAVDESMILNQYNLRDYRWDSSGNFLTFEYNKRGHQVYRVMKIDATTGKMSVIID
                     EQSPTFIDYSGKRYRYDVKETGEIIWASERDGWNHLWLYDGNTGRVKNQITKGEWPVR
                     RVVSVDVKERSIIFTASGREKGDPYLIHYYRIGFDGTGLTHLTPADGNHEALFSENGN
                     YFVDTWSRVDMPPVSVVRSTADGSEVMKLAEADVKDLMATGWQYPEVFTAKGRDGITD
                     IWGIIIRPLNFDPSRKYPVIENIYAGPHSSFVPKSFRTTHSNMNSLAELGFIVVQIDG
                     MGTSNRSKKFHDVAWKNIKDAGFPDRILWMKAAADKYPYIDIDRVGIYGTSAGGQNST
                     GALLFHPEFYDVAVSSCGCHDNRMDKIWWNELWMGWPVDQSYIESSNVENAHLLQGKL
                     LLINGEMDNNVDPASTTQLVNALIKAGKDFDYLLVPGMQHSTGGVYGERRRMDFFVEN
                     LLGIKPPDWNKK"
     gene            4729..5673
                     /locus_tag="PLA17_00800"
     CDS             4729..5673
                     /locus_tag="PLA17_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPK83980.1"
                     /translation="MTDRRTNTIIWAIITCLMWSTAYPFIKIGLQYSTPVHFAGTRFA
                     LAGLMILPFTVKPREYIRMIRANSSLAIWVTILQTLINYTLFYLGMDRVPGALGAVIV
                     GSQPFVTAIVSRIMIREERFTRAKIITIILGLAGIVLVSAGRMEFHFGMPGEIAGVIM
                     IFIASISTATSNVLISKKGKNMNPLVLSSFSLLTGGLIMFFLSFGFEDVPLKPDFPLN
                     YWLVLIWLSFMSAFTFSKWYVLLKRPGVRVSELNLWKFIIPVFGAILSWIIVPGEKPD
                     WVTVTGMIIISLSLIWFFGFTTKREHKIGKVPAGPIAK"
     gene            complement(5841..7094)
                     /locus_tag="PLA17_00805"
     CDS             complement(5841..7094)
                     /locus_tag="PLA17_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK83981.1"
                     /translation="MQPEVNMKGEGKQDSVSGKELHGSKIFGFERNILFTGLTSFLTD
                     TSVKMIYSVMPMFLMSIGASKASLGLIEGVAESTASLIKALSGFWSDRIGRNKPFMLI
                     GYAISAIVLPLYAFVISPMQVLFLRFTERIGKGIRTAPRDSLIAASVTNNETGKGFGL
                     QKAMDNSGAIAGPLMAFALLSLFPDNYRLIFLIAGIPAIAGIFVIIFGIKEVKKNKSE
                     LLQKFRFTDYPKRYWFFLAIIFVFTLGNSTDALLLVKANEAGIKVAFIPLVYLITSLV
                     SVIAAIPAGTLSDRIGKEKLLVAGFLIYAVVYLFFGLNVGEGALFVLFALYGLYSAAT
                     DGIQKAFVADVTDANKKGTALGIYNALLGITLLPASLIAGILYDRVNSSIPFLFGAAM
                     ALLATIMMAVYMITGRKQPAKGSRG"
     gene            complement(7276..8484)
                     /locus_tag="PLA17_00810"
     CDS             complement(7276..8484)
                     /locus_tag="PLA17_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPK83982.1"
                     /translation="MEKPFILARIPQVLFRSGVICELPAIVEGYGKNIVLVTGAGSFM
                     SSGRAERLLGQFRARGIKVRHVTVAGEPSPGVVDEAVSRLKGAGISLVVSIGGGSVMD
                     AGKAISAMLTTDGSVTDYLEVVGSREHPGTKVPFIAVPTTSGTGSEATKNAVISQVGK
                     EGFKRSLRHDNFVPDLAIIDTELTLECPPDITAAAGMDCFTQLTEAFLSDKGSPITDA
                     LAIEGLKAVSRSLLRSFTNGDDKEARSDMSFAALMSGICLANAGLGAVHGLAGTIGAL
                     YDIPHGVVCGTLMAPANEVNVRELRRVSRSGAVTDRYSAAVTSAALGKYATLGRIFAD
                     ATGKNDNYYIDFFTDCLNALSYRLRMPKLSRYGLVHDDLAAICSLSDVKNNPVCLSEE
                     QMLEILMSRI"
     gene            8762..9736
                     /locus_tag="PLA17_00815"
     CDS             8762..9736
                     /locus_tag="PLA17_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83983.1"
                     /translation="MKMKFLCTIACVLAFSAFFASGLNAQDRNEVIAVYNEGAKAIQT
                     DVPAAITAFEKVIEMSDQVGESVDDLKQKVIQVLPGLYYKLAATAYNEKKPVAEIIAA
                     SKLAGAMAEKYGSKTHGNNSQKILVNGYYMMATEFFSANDYDNALLAFDSVLTLNPDH
                     IASIYNKSLIYKNRDEADLFEQTIDLFLSKLDPGKDADKAEQAKGSALEYFRAAGSKA
                     NSANDLDEALALLNRAAKYGEDKILCYFFADVYNKKNSFNEGAGYAQKGLDMETGDAE
                     AKAMFYYQLAVAQVGKGETSAACESFKNALYGQFEEAAKAQRTNLKCQ"
     gene            complement(9825..15806)
                     /locus_tag="PLA17_00820"
     CDS             complement(9825..15806)
                     /locus_tag="PLA17_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPK83984.1"
                     /translation="MKSTFFLRSLLRYLPLLVLPMLSGVTYGQDLLHSRQSSFYTYIY
                     KINDREAEQIYKNNLWEVDQKFFHTLVDSFPTDSAFVKNLPIGHYLRVYTEKNRLRID
                     ITSVQDFDVMILNNNTDLAVQVYNLKGEIIPDADVSVRLKSIRFDSKTQSYLDRKSNQ
                     KGLLKVNHNGFTAFYDLKREYNNSSIKRTARKIVYGTPVKYVWYPVRYIVFLPIDGVR
                     SISHGYPQGTIQRTSWFFRKSFYKIMCLFDDSYCDYYSTGSNYKGYMVFNKPKYMPRD
                     TVKIKAYIVNKSGRAVKDDVKVVLKGPRKHVTLTTLMPYKQGGYSYEFYLHDSLNLLL
                     DQNYRVSFENNKEREYISGTFKYEDYELRSIQLKLIADTTEHFHGKNVIVRVKGTDEN
                     GLNILDGRLEVLVKPEEVQKYFGSYVFIPDTLAYWQLDLEKENETSITVPDSIFPRAN
                     LSYDVVVTLLNSDNETVTKTEHLTYLFSKSEIRYDLTNDSIEFFYQKNGVRSPVRARI
                     LGIDNFGNETTIMEGWLPLKTGINPYYSAYCADGDSVKTKVSLSTEPSMLQCFSERSR
                     DSIRIMVNNPRKLPFSYFIYKKNSEKARGHSDSLNLHILTSGKENYFLAIQYLWGGKL
                     VDENYKIPYHDKKLNITVNEPKIVYPSQTSAIEILVTDPEGNPVPDVDLTAYSITGKF
                     DYTPPSLPYLGTQRKNKSIINNFSINEHLFDYFQGLNLDYNYWRLWAGLDTIEYYNFI
                     YPGNKIYRFQYDTETTQFAPFVISGGQPVPIHVIYVDRKPIYFSWSTNIQPYSFSVDS
                     GYHHVKLRTTQRDFEIDSVYFNYHTKTILSLRDSIINKNVQVNKVKPLLSDYEKAILY
                     KYIFPYRYQFGEYYGYIEQNSRVFFLKPEKSGPNYNLAGPVDPTGTKFQLVDSFSVGF
                     THEPFFEYDFAPGLLKMRNIDPKFRYPGYLYNYPARESMADKVLNAEDINKGWNDYIN
                     QKRYLTARYNYPGTTSAGMGTMKIDLLADGNKTSLRPLNILLFRYDESEFLRIYPGSE
                     FTFNDLDKGYYKVLFFLPGSEYSVIDSLFVTPNGLNYHRIELPEKHIKDAFSTSVSKI
                     IEENIFKPVSNPIRQEREIKQIYDRYQQEFRFTGAGDIIEGYIHDEEGNPIPGVTVLV
                     KGTTYGTISNLDGYYSLNVPREKNTLTFSFIGYQQEEIDISDKKTVNVNLTPEALNLQ
                     EVVVIGYGVREKSSLTGSVSVVNTAGIPGGMESALTGTLAGKVAGVEITRSDVPDSVV
                     SIHIRGSGTTNFNGEPLYVIDGMVFTGNINELDTDLIKGIQILKDSQATAIYGARGAN
                     GVVLINTGGIFLATKSPGQLGAEYDEAFLEAASQSSSIRNNFSDYAFWQPELVTDKEG
                     KASFEVTFPDDVTSWKTFYLAMNGKKQSGQTEGVIKSYKPLMGQLSVPGFLVAGDSTL
                     ALGKVLNYTPDTIPLRTKFEIDGNLISVKEQNCIRSIIDTLPIVACPDDSVSVKYYLE
                     KDDGYFDGEHKHIPIFPPGLEETAGQFFVLDRDTTINVSFSPISGDAKVYARADVLEV
                     LEEEIIKLIAYRYSCNEQLASKLKALLSEQIICKFKDKAFKKSNEVERIIRLLNRSKQ
                     EDELWGWWKSSKESSLWISLHVLEALTKAREMGYRVNLNENKIADNLLWEMSRTNLIQ
                     SKLSAIQILKTLNATVNYPALISDAEKSGELTMNQLFQLIELKQICGIKVSLDTLKSF
                     RKETMFGNIYFASGKVTSGLLTNNVQNAIIAYRIISRDTAVNHDRTLLKIRNYFFENR
                     STGFWLNTYESSKIIETILPDLLTSKEQVIKPKLVISGAIDKETDEFPYEVTVSSDDT
                     LEITKSGDFPVYLTAYQRYWNPDPLEKKSDFEISTSFIDTDNNVLKAGKEVKLRVQLK
                     VRKDADYVVVNIPLPAGCSYLNKSKSSRWEVYRESFRNETVIFCEKLMPGDYEFTIDL
                     MPRYTGKYALNPAKVELMYFPTFNANNSFRNVTIE"
     gene            complement(16131..16204)
                     /locus_tag="PLA17_00825"
     tRNA            complement(16131..16204)
                     /locus_tag="PLA17_00825"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16168..16170),aa:Met,seq:cat)
     gene            16391..16777
                     /locus_tag="PLA17_00830"
     CDS             16391..16777
                     /locus_tag="PLA17_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HPK83985.1"
                     /translation="MRTKFEIEFTLNTPVRVLYDRLSTPEGLSEWFADNVTVDGDIFT
                     FHWNKSEAKARLVYARENKAVRFDWLDVTDTETNFFEFRIIVHELTNDTALIITDHAE
                     EDEIEDSRELWNTEVSYLKKILGITE"
     gene            16832..18331
                     /locus_tag="PLA17_00835"
     CDS             16832..18331
                     /locus_tag="PLA17_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPK83986.1"
                     /translation="MKKIDLFMIRSFIGPLVLTFFIVLIIFILQFLWMYVDDLAGKGL
                     ELKVLAELLFQFSLTFVPLSLPLAILLASLMTFGNLGEFLELTALKSSGVPLQRIMMP
                     IFIFSSLLAIGSFLFADYILPISTRKARTLLTDIRRMRPELNIQAGTFYNGIDGFSIK
                     ITDKDPETNRLDGIYVYDHRDGVGNSSVIYADSGYMNVTPDESGLILTLYNGHAYKDI
                     EEKDVQQDKRKYPFRRDRFSEQTMVVELEGFGLNRSGMDIYRSNYAMLNTADLTYFID
                     SLSTMLRSRADAYYSEFNQTRLLTTPHFRLRGINREEDSLAAWKTQKFVARAAFDTLS
                     FNDKGTTISRALSFAREGASFMIEKSESIVAEAKNLRKYEAERYKRFTLPFACLVFFF
                     IGAPLGAIIRKGGLGTPAVISVLFFVIYYVITLSGEKFAKELIINVHLGMLASTIILL
                     PIGAFLTYKATTDAAIMNAETYIAFFRKIVAFLTGIKPEKDDEGTGAGA"
     gene            18303..19514
                     /locus_tag="PLA17_00840"
     CDS             18303..19514
                     /locus_tag="PLA17_00840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK83987.1"
                     /translation="MMKVLVLAPRPLWPAHDGGTVASVHCVSGLAAAGAEVTALSMVT
                     EKHMTSGIYACGAKPDYLTDYITVQVNTAIRPLPMILNLLFSGEPYDLARFRSGEYSK
                     ALRRILTGNQFDIIHCEGLPLVLYLNGIRSLTTTPVVLRAHNLEHTIREMMAATACCP
                     LRKYYLSLLSRRLKKTEAKTARQFDAVVPISEPDAAWFRSISGDKPVFLAEAGTDEAL
                     MLPEPPQENPRVGFIGAMNWQPNADAIKWFISEVWSGVIRRIPSATLHVAGRGLRQTD
                     VLPPGPNIINVGEPDNAREFIASNHVMIAPLFAGSGIRIKIIEAMSAGRPVVATPVAV
                     AGLPVENGRELAVASDPATFTAAIVRLLEEPELRASTGERALRLVKERYDNTVITARL
                     LEFYKKLRHGS"
     gene            19504..20670
                     /locus_tag="PLA17_00845"
     CDS             19504..20670
                     /locus_tag="PLA17_00845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK83988.1"
                     /translation="MAAEIILAITAALLAWTYLIYPAAVIMAANLKKRHTLPSAVALE
                     SLPSVTVVMSLHNEKEVAEKKIRSMLASEYPAEKLDFIIGSDGSDDGTGAVITAMAAT
                     NPNIQIIINAERKGKAAMLNELVSGAKGDILVVTDANVMFTPSVIRKLAEGFADSRTG
                     LCDATVHPFSADDTGITRQEIMYSRFETALKRAEGLLWGAMPGPYGGCYAVRRSLFPR
                     LPENTLVDDLFVGLSVLKENYRAFNIPDAVVFEDTQPDMIEQYRRRTRIAAGSFQNLF
                     RFGPCISERAGALFAFLSHKIMRWLTPLLLVLFFMTTVILSGSSYFYFCLTAFQLIFI
                     ILSVIDIMLDRRGKKLFIQRYVTQFLLMNAALATGFVKAVRGIESGIWEPTKRV"
     gene            20672..21916
                     /locus_tag="PLA17_00850"
     CDS             20672..21916
                     /locus_tag="PLA17_00850"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPK83989.1"
                     /translation="MTDDTKLNTINEAIEEIREGRVVIVVDDEDRENEGDFICAAELI
                     TPEIVNFMATHGRGLICVPLPEERCEEMELDMMVGHNTSLQETPFTVSVDLISPETTT
                     GISAKDRALTIKALVDPSTKPGQLGRPGHIFPLKARSKGVLRRAGHTEAAVDLTRLAG
                     LNPGGALVEIMNEDGTMARLPDLIRLSEKFGIKIISIKDLIQYRLGLESIVEMGERVK
                     LPTEYGNFDFIPFRQVSNGLEHAALVKGHWTKEETVLVRVHSSCFTGDIFASKRCDCG
                     QQLHRAMQMVEAAGKGAIVYLNQEGRGIGLINKIRTYKLQEQGRDTVEANIELGFRED
                     ERDYGIGANIMRDIGLGKVKLISNNPVKRAGLEGYGISIVETIPLEITPNKYNSFYLE
                     TKARKMGHSITCYKTRAKGKND"
     gene            21909..22868
                     /gene="fmt"
                     /locus_tag="PLA17_00855"
     CDS             21909..22868
                     /gene="fmt"
                     /locus_tag="PLA17_00855"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPK83990.1"
                     /translation="MTEPTLRIVFMGTPEFAVASLNALYETGVDIAAVVTAPDRPCGR
                     GRKITFSPVKKYAMDHRLTLFQPEKLRDPEFISALRDINADLFVVVAFRMLPEEVWSM
                     PRLGTFNLHASLLPQYRGAAPINHAIINGETMTGVTTFLIDEDIDTGMILLSRETAIL
                     PDETAGSLHDRLMETGARLVVETARGLAAGSLKPAPQEKMDDTMLKPAPKISPADMVI
                     RWEKIAENIHNLIRGLSPCPGANTMLRKEGRSLRLKILDSTINYEITGNIPGTLIVTE
                     KSRLMVACGKDTLEILSLIPEGRKRMSAAEFLRGFDLSGWEMA"
     gene            complement(22875..23969)
                     /locus_tag="PLA17_00860"
     CDS             complement(22875..23969)
                     /locus_tag="PLA17_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HPK83991.1"
                     /translation="MKNLFIYLVLISLLLFNSCSPARRTARSTASTAPATDYAREYLI
                     KYGNLAVSEMKRSGVPASITLAQGMLESNYGRSRLATLGNNHFGIKCHSDWSGKRIYH
                     DDNRKGECFRSYASPEESYRDHSDFLVNGSRYRNLFHLAATDYKGWAHGLKKAGYATD
                     PKYPELLIRKIEDYSLWAYDTGGTSPIVSQQAAGSQPAGSGTVPAAATSSGTTVTPRP
                     AVKETGTGQAQPVPDKRATAIEDDEPVRVISISTGAKTLENNNVEYIIVEEGDTYESL
                     AERHQLLSWEIVRFNDLTAGAPLIPGQVLYIKPKRTRAAQGLLVHTVEAAETMHSISQ
                     KYAIRLSSLYKMNLMDEGTECRPGQKLRLR"
     gene            24079..24564
                     /locus_tag="PLA17_00865"
     CDS             24079..24564
                     /locus_tag="PLA17_00865"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPK83992.1"
                     /translation="MKKRESTISFLEYNSEDELPAMEKELVGEAVRAASGAYSPYSHF
                     MVGAALLLDSGEIVKGANVENAAYPSGSCAERTALSYSVANFSDRKVVAIAVAALTDG
                     VLTEDPVTPCGNCRQMLIEEEQRNGRPIKVILAGKSAIYVLENSQGLMPLSFTASNLS
                     K"
     gene            complement(24681..25373)
                     /locus_tag="PLA17_00870"
     CDS             complement(24681..25373)
                     /locus_tag="PLA17_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPK83993.1"
                     /translation="MSRKNARIFLVEDDASFGSVLKSYLEINDFEVDLVDDGKVAVET
                     FRKGVYDLCILDVMLPHVDGFTIASEIRIMNSVIPIIFLTAKKLKEDLLKGYGVGADD
                     YITKPFDTDILLCKIRAIIARRDIKDGTRDIYEIGKYVFNSRLRTLTLGDRERQLSPK
                     EGQLLEMLAISPNTLVSREATLRKIWGSDDYFTARSMDVYVTKLRKYLSDDPNILLKN
                     IHGSGFQLVILE"
     gene            complement(25370..26920)
                     /locus_tag="PLA17_00875"
     CDS             complement(25370..26920)
                     /locus_tag="PLA17_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPK83994.1"
                     /translation="MNMRKFTGLIVLMVVSVVAIVWVQVTWISNSVRAQNEQFDFFVI
                     NTLRNTAHSMETNRRMNFLNEMFIRGYSSLPQTIERTALQSHMTGNYSFRSQINSDSD
                     SVEIIVSENENPPVRMKVTRQEASEKMGSSIVVGSDEYMRWMQQQANEFQLMSNQLAS
                     EMFVWERNKEINKEELLQTLKGELAASGINTPFEFAIIRGDSIIDGVFDKVKGKDFLD
                     SPYKVSLFTQGLFRRGETLSVVFPRKTNYVLGTMGLMLGGFGLFLLIILSTFALSLWF
                     IIRQKKTSEMQADFINNMTHEFKTPIATISLAADTIANSRVITDENRVRHFISMIKKE
                     NLRMNRQVESILQIATLDKREMDFRFAPVDLHEVICQAIDTIEIQVADRGGVVHRHLD
                     AERSIINGDTEHLRNLVHNLLDNANKYSEKSPDITVKTRSNDSGIFLQVADRGIGMSK
                     SVQNRVFERFYRETTGNIHNVKGFGLGLNYVKAIAEAHGGTITVESEQGEGSVFTIFL
                     PFSNETRA"
     gene            27052..27183
                     /locus_tag="PLA17_00880"
     CDS             27052..27183
                     /locus_tag="PLA17_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK83995.1"
                     /translation="MTDKSALNGYMNSGSHLCGTITLSAQEIENVRMKAYKKPEQHL"
     gene            complement(27352..28038)
                     /locus_tag="PLA17_00885"
     CDS             complement(27352..28038)
                     /locus_tag="PLA17_00885"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPK83996.1"
                     /translation="MSGEFPEVLYEDNHIIIVNKRSSDLVQGDGTGDESLDNIIRRYI
                     KEKYGKPGNVFLGVVHRLDRPVSGCVVYARTSKALSRLSELFRTRDVKKSYWAIVTDR
                     PPAEEGSLQNYLKKNEKQNKSYVYDKEVKGSKSAELSYRILARSERYYLLEIDLLTGR
                     HHQIRAQLAAAGCPVRGDLKYGSRRSNEGGGISLHARRVSFIHPVRNEEINVVAPLPD
                     DRIWKLFKNV"
     gene            complement(28096..>28209)
                     /locus_tag="PLA17_00890"
     CDS             complement(28096..>28209)
                     /locus_tag="PLA17_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPK83997.1"
                     /translation="PRFLRRYHNLYDEIKGAVQHYIADVRSGSFPNEKEQY"
BASE COUNT         7543 a         6958 c         6437 g         7271 t
ORIGIN      
        1 ttttataccc agaacggtgc tgaagatcac acctgccaca tactcaagcc ggggggagag
       61 agccggagag taaatccata gctttctggt tctcatatca gcaaatttaa taaatcggcc
      121 ggttaccctc cccgtatact catccttttt attaacagaa tgtgatgtcc ggggagtaaa
      181 ttttactaat tttgttgttt gcgcaatttt gtgttaacaa ttaaatattt caaagaatat
      241 gggcaaaggt gacagaaaat caaagagagg gaagattata aaaggatcct ttggcgtccg
      301 ccgtcgcagg aaagtcagaa agagttttgt tgcaccatct ccgaaggaga cagtgaaagc
      361 aactccggca ataacaccgg aggcagagga agcaaaacct gccaggacag ccaggcccgc
      421 gacagttgca aaggcagcca cgaaacctgc agctaagaag agtgaaaagg agacaggaac
      481 cactgctgaa aaacccgcaa aggctgcaac caaaacaaaa aaacctgctg ctaaaaaaga
      541 agaggaataa ccggtatctc agctttttac gataagatca tcatatactg acggattgat
      601 ggcggcctcg atgatttcac agcccccgga ggcaagggca cgtttaagcg ctgaacgcat
      661 tccggagagg tcagtgacat actgcacatc aactcccagg aatctgtcag ctccgaagag
      721 agagccgtca tgaacatgta tgccatacgg gctcaggctc ttccatgact gtttgacctt
      781 gataaggttg agttccccgt cactgaagac gactacgata attttcagtc ccagccggcg
      841 tgccgtcata atctcaccgg cagacatcgc aaacccgcca tcacctgtca cgcacactac
      901 aggcccgccg ccgcctgcca gggcagccgc aacagcggca ggaagcccga atcccatcga
      961 tgaccatccg tttgtcatta tcagctttcc gcgctgcaga tcccacatct ggcctgccag
     1021 gtggaggtgt gatcccacat ctgccgtcac agtggtatcc gaaggaagga gttcacgcag
     1081 caacgacagg gcagtaacag gattcaatcc actccctgcc cttttaaggg atgtccgtat
     1141 ctcatccctt gtccgggctg ccagggccat catctcgtca gagcttccca gggacgacaa
     1201 taccatgaac cactcctccg gggctgacac aacctcaatt gctccgctga tccgcaggtc
     1261 tgaaggcttt gtgtcaaaat ggaccagagg cacctccgga agccatgatt cgtaattata
     1321 ctccaccgga tcataaccca gaccgataac caggtcggct gcccttatca gccctgcaag
     1381 ccggtcactg agcgcatgga acaggactcc ggcaaaggat tgatgatcat gagtgataac
     1441 tccctttgcc attggtgtca ccactaccgg aactttgtgc tcttcgagaa atcttaccag
     1501 cgaacggccg gcaaccgttc tcatggcagt caggccgacg gcaatcaggg gccttcgcga
     1561 tgaagctatc aggtctcccg ccctggcccg gttatcttct cccgtggaag catgccctga
     1621 aattcggacc ctggccccac tggctcccgg tacagaagta tgccttgaaa ttccggcccc
     1681 gctcccgctg cctccacccg cagaagcatg ccatggagtt tccaccccgg ctccgctgac
     1741 tcctgctgtg gaatctatcc ctgaaattcc cgcagaccct gaagcgcctg tatacggtcc
     1801 ggcaacactg cctgccaggt cagagggcaa ccccatatgc acaggtccgg ggtactcttc
     1861 atttgccagt tcaagagcgc ggctgataat ctcccgtgca ttatcagcat tgagcctgaa
     1921 ggtctccttt gtcagcggac gaaacagaac ctgatgatcg atattcatct gggacgtacg
     1981 acccagccat gcctcagaca tctcagtggt cagggcaatc agcggggaac ggtcaagcaa
     2041 tgccccgccc acccccgatg aaagatttac tgctcccgga ccaaaggtgg catggcacac
     2101 acccgtgaca ccggtaagcc tggccgtgac atctgccatt accgcagccg atgcttcatg
     2161 actggcaagg ataaactcta taccagcctt cctgaaggcc tccagatacg gtatggaggg
     2221 tccgcccggc accccgaaaa acctccttac tcctgcttcc cgcagctgcc gtgctaaaaa
     2281 atcctcaata accataacct ctctccttcc ttaatacagg aacatttaaa aaatgtgaaa
     2341 acatggtaaa gtaaaccata aatcaatcgg gaagtgttca gaatacacgc attttatctt
     2401 aactatgcat aaaaataaac cagtcaggca atgaagagaa cttttatctg tgtttttttg
     2461 tcttttctgt tcgccgccac gctaagtcat cagggcagtg cgcagggaac cgaaaaggat
     2521 tatgagaggg ctaacaatat tgacagacag cttgccggaa aggtatggca cagtgacatc
     2581 aggcctgtct gggtaggcaa gaccaatatg ctggtctttg aggacagaac cccggaaggg
     2641 aaggattata tccttgctga tattctgaaa aaaacaagta aaaaggcatt tgatgccgcg
     2701 aggctggctg atgcccttgg aaagatcacc cgtgacacag tcagggcagc cgatctcccg
     2761 ataaggaacc tggtaatggc tgatgatctg aagtctgtga cctttgagta ttccggagcg
     2821 atgtacaaat gcgacctgaa aaaatatacc gtcaccaggg agcgtgaaac cagaagacct
     2881 tctgcatggg gcggagagga aaggtggccg tggggcttcc gcaatgaact gatcggaagg
     2941 cctgacacat cgcccgacaa aagcatgacg gcattcattc gtgattacaa cctctggata
     3001 agggtgctga aggacaagaa agagtaccag ttgagccatg acggcgggat aggcaaatac
     3061 tactcggcat cggtttactg gtcgcctgat tcgaaaaaaa taatggcaaa cctggttgtc
     3121 cctgccgaaa aacacatgat ttcatatgtg gagtcgtcac ccgaagacca gatccagccg
     3181 aaatactata cccgggaata tcccaagccg ggcgatgccg ttccccagtt cttcccgcag
     3241 atatttgatg ttgccggaaa gaagcacatc gccgttgatg agtcaatgat cctcaaccag
     3301 tacaatctca gagattaccg atgggactct tccggcaact tcctcacttt tgaatacaac
     3361 aaacgcggac accaggtcta cagggtgatg aagattgatg caactaccgg gaaaatgagc
     3421 gtgatcattg atgaacagtc gcccaccttc attgattaca gcgggaaacg ctacaggtat
     3481 gacgttaagg aaaccggtga aataatctgg gcttcagagc gcgacggatg gaaccatctg
     3541 tggctctatg acggcaatac cggcagggtg aagaaccaga tcacgaaggg agaatggcct
     3601 gtgcgcagag tggtcagtgt tgacgtgaag gagcgcagca taatctttac ggcttcaggt
     3661 cgcgagaagg gagacccata tctgattcat tattaccgga tcgggtttga cggtacggga
     3721 ctgacccatc tcacccctgc tgacgggaac catgaggctc ttttctcaga aaacggaaac
     3781 tattttgttg acacctggtc acgggttgac atgcctcctg tctcagtggt gcgcagcacg
     3841 gctgacggca gcgaggtgat gaaactggcc gaagctgatg taaaggatct gatggccaca
     3901 ggatggcaat accctgaggt gttcaccgcc aaagggcgtg atgggataac agacatctgg
     3961 ggaataatta tcaggccgct taacttcgat ccgtctcgga aatacccggt tatcgagaat
     4021 atttatgccg ggcctcacag cagcttcgtg cccaaatcgt tccgcacaac gcacagcaac
     4081 atgaactcac tggctgaact gggtttcatc gtggtgcaga tagatggcat gggaacatcc
     4141 aacaggtcga aaaagtttca cgatgtggca tggaagaaca taaaggatgc cggtttccct
     4201 gacaggatac tctggatgaa agccgcagca gacaaatacc catacattga tattgaccgg
     4261 gtgggtatat acggaacttc ggcaggcgga caaaactcaa ccggagccct cctcttccac
     4321 cctgaattct atgatgtggc agtttcatca tgcggttgtc atgacaacag gatggacaag
     4381 atatggtgga atgaactgtg gatgggatgg ccggttgacc agagctacat cgagtcgtcg
     4441 aatgttgaga acgcgcacct gctgcaagga aaactgctgc tgatcaatgg cgagatggac
     4501 aataacgttg accctgcttc aaccacgcag cttgtcaacg cccttataaa ggcaggaaag
     4561 gactttgact acctgctggt gccaggcatg cagcacagca ccggtggcgt ctatggcgaa
     4621 cgccgcagga tggatttctt tgttgaaaac ctgcttggaa taaaaccacc ggactggaat
     4681 aaaaagtaag aagggaaaaa gcgatatttg catttttatc tgatccggat gactgaccga
     4741 cgtaccaaca ccatcatctg ggcaatcatt acatgcctga tgtggtcaac cgcttatcca
     4801 tttattaaga taggactgca atattccaca cctgttcact tcgcaggaac caggtttgct
     4861 cttgcgggcc tcatgatcct tccgttcacg gtaaagcccc gggaatacat caggatgatc
     4921 agggccaaca gcagtctggc tatatgggtt accatactgc agacgctcat caactatacc
     4981 ctgttttatc tgggcatgga tcgcgtgcca ggcgccctgg gtgcggttat tgtaggatcg
     5041 cagccctttg tgacagcaat agtatcacgg ataatgatca gggaggagcg gttcacacgc
     5101 gcaaagataa taaccattat tcttggtctg gccggaattg tactcgtcag cgccggacgc
     5161 atggagttcc atttcggcat gccgggcgag atagcgggag taataatgat ctttatcgcc
     5221 agtatttcaa cagccacaag caacgtcctt atctcaaaga aaggcaaaaa catgaatccg
     5281 ctggtgctga gctcattctc tctccttacc ggcggtctta tcatgttctt cctctcattc
     5341 ggatttgagg atgtgccttt aaaacctgac ttcccgctga attactggct ggttctgata
     5401 tggctttcct tcatgtcagc atttaccttc tccaaatggt atgtacttct caagcgtccc
     5461 ggggtcaggg tcagcgagct gaacctctgg aaattcataa ttccggtctt cggcgcaatc
     5521 ctgagctgga taatagtccc gggagaaaaa cccgactggg tgaccgttac cgggatgatc
     5581 atcatttcac tttcactgat atggttcttc ggcttcacta caaagcgtga acataagatc
     5641 gggaaagtgc ccgccggacc gattgccaaa taaggatatc ctgaaagctt tgccgtatga
     5701 cgctactgaa aggctggcgg gctgacactc ctgaaagcct tgcggtatga cactcctgag
     5761 aggatttcgg gctgacactc ctgcaagcct tgcggtatgg cgctcccgga acctgtttca
     5821 atgaatatat gcaatagata ttatcctctg ctgccttttg ccggttgctt tctgcccgtt
     5881 atcatgtaga ccgccatcat gattgtggcc agcaatgcca tggcagctcc gaacaggaag
     5941 gggattgatg aattgaccct gtcatacaga atacccgcta ttaggctggc tggcaggagg
     6001 gtgatcccga gcagggcatt gtaaatgcca agagctgtcc ctttcttgtt ggcatctgtc
     6061 acatcagcca caaaggcctt ctgtatgccg tcagtagctg ccgaatacaa tccgtaaagt
     6121 gcaaagagca caaaaagagc gccctcaccc acattcaggc caaaaaagag atataccaca
     6181 gcatagatca ggaaccccgc cacaagcaac ttctctttac cgatcctgtc ggacagcgtt
     6241 ccggcaggta tggcagcaat aactgacacg agactggtta taagatagac caatggtatg
     6301 aaagctacct ttatccccgc ctcattggct ttgacgagca gcaatgcatc agtcgagttc
     6361 ccgagggtaa aaacaaagat gatggcaaga aagaaccagt accgtttcgg ataatccgtg
     6421 aagcggaact tctgcaaaag ctcactctta ttctttttta cctcttttat cccgaatatt
     6481 atgacgaaaa tcccggcgat ggctggtatc ccggcaatca ggaatatgag ccggtaattg
     6541 tccgggaaca ggctgagaag agcaaatgcc atgagcgggc ctgcgatggc tccgctgttg
     6601 tccatcgcct tctgcagacc aaatcctttg cctgtctcat tatttgtgac cgaagcagca
     6661 ataagactgt cacggggagc cgtacgaatg cccttaccaa ttctctctgt aaacctgaga
     6721 aacagaacct gcatcggtga tatgacgaag gcatacagtg gaagaactat tgcggagatg
     6781 gcgtagccga tcagcataaa tggtttgttc ctgcctatcc tgtcactcca gaaaccagac
     6841 agtgccttga taagagaagc cgtgctctcg gcaacgcctt ctatcagtcc cagacttgcc
     6901 ttggaggcgc caatagacat cagaaacatc ggcatgaccg aataaatcat cttgacagat
     6961 gtgtccgtaa gaaaactggt cagaccggta aagagaatat ttctctcgaa cccgaatatc
     7021 tttgatccgt gcaattcctt tccggacacg gaatcctgtt tgccttcgcc tttcatgtta
     7081 acctcaggct gcaccagcag ttctgattat tgtcgtatac ctctgtccgg gtatcggccg
     7141 gactaaatca agagatcaaa attatcagtt taaaaccgat tgtagcggga ttttttatct
     7201 gaacctgaag aaaattttaa agcaatgata caaggcttat cttcatgact gattctgtgg
     7261 cgtgacctcc ggatttcaga ttctgctcat gagaatctcc agcatctgtt cctctgacag
     7321 gcagacagga ttgtttttca catcagatag tgaacatatt gccgccaggt catcatgcac
     7381 cagtccgtaa cggctcagtt tcggcattct gaggcggtaa gagagtgcat ttaagcaatc
     7441 tgtgaagaag tcgatataat agttgtcatt ttttccggta gcgtcagcga aaatcctgcc
     7501 cagggttgca tatttcccga gggcggcaga ggtgaccgcg gcggaatacc tgtccgtgac
     7561 ggcccccgat ctgctcactc tccgcagttc ccgcacattc acctcatttg ccggagccat
     7621 cagtgtgccg cagacaacgc catgagggat atcatacaat gctcctattg tacctgccag
     7681 tccgtgaact gccccaagcc ctgcattggc aaggcagatg cctgacatga gggctgcaaa
     7741 tgacatgtcg gatcttgctt ctttatcatc accgtttgtg aatgatctca ggagcgacct
     7801 gctgacggcc ttcagccctt caatggcaag ggcatcagtt attgggctgc ccttgtcaga
     7861 cagaaaagcc tccgtaagct gtgtaaagca atccatccct gcagcggcag ttatatcggg
     7921 cggacattca agtgtaagct ccgtatcaat gattgccagg tcaggcacaa aattgtcatg
     7981 acgcagtgat cgcttgaagc cctccttccc cacctgcgaa attaccgcat tcttcgtggc
     8041 ctcactgccg gtacccgaag ttgtgggaac agcaatgaac ggcacttttg ttccgggatg
     8101 ctccctgctg ccaaccacct caaggtaatc agtcactgac ccgtctgttg tcagcatggc
     8161 cgatatggct ttcccggcat ccatgacgct gcccccgcca atgctcacga caagtgagat
     8221 accggctccc ttcaaccggc tgacagcctc atcaaccacc ccgggcgacg gctccccggc
     8281 aacagttaca tgccggactt taattcccct ggccctgaac tgcccgagga ggcgttcagc
     8341 acggccggat gacataaatg accctgcccc ggtgaccaga acaatgtttt tgccatagcc
     8401 ttcaacaata gccggcaact cacagattac accgcttctg aataaaacct gaggtatcct
     8461 ggcaagtatg aacggtttct ccataactac ctgtcttaaa gttcgggaat gtcatttttc
     8521 ttctcccata ttgatgcaca ttattgagag atttaaaaat acattttatt acggatctgt
     8581 attaaacaac gagttcagac tgcttcttcg catgaccggt tttatactgc aatcagtgca
     8641 tttcaatctt tatggccctt tttttgttat gagttttgcg gatgcctgaa ttattgatca
     8701 tattttctat atttacgggc tgataaagaa ctttttttat aattcttaaa ataaccagaa
     8761 aatgaagatg aagtttttat gtacaatagc ttgtgtgctt gctttttcag cgtttttcgc
     8821 ctccggtctc aacgcgcagg atcgtaatga ggttattgca gtttacaacg agggcgccaa
     8881 ggcgatccag actgatgttc cggcagccat aactgctttt gagaaggtga ttgaaatgtc
     8941 cgaccaggtg ggcgaatctg tcgatgatct taaacagaag gttatacagg tactccccgg
     9001 actctactac aagcttgccg ccacagccta caacgaaaag aaacctgttg ccgagataat
     9061 tgcagcctcg aaacttgcag gtgccatggc tgaaaagtac ggaagtaaaa cccatgggaa
     9121 taattcacaa aagatacttg tcaacggtta ctacatgatg gcaactgaat tcttctctgc
     9181 caatgactat gacaatgcac ttctcgcatt tgacagcgtg ctgacgctga atcctgatca
     9241 cattgcctct atatacaaca agtctcttat ctataagaac cgtgatgagg ctgatctctt
     9301 cgagcagacc atcgacctgt tcctgtcaaa acttgatccc ggaaaggatg ctgacaaggc
     9361 cgaacaggct aaaggctctg cacttgaata tttccgcgca gcaggatcaa aagccaacag
     9421 tgccaacgat cttgatgaag cgctggctct tctgaacagg gcagccaagt atggcgagga
     9481 caagatcttg tgctacttct ttgccgatgt ctataacaag aaaaacagct tcaacgaagg
     9541 tgccgggtat gcacagaaag gactggacat ggagacaggt gatgcagaag ccaaggcgat
     9601 gttctattat cagctcgccg ttgcacaggt gggtaaaggt gaaacttctg ctgcatgcga
     9661 gtcatttaaa aatgcactct acggtcagtt tgaagaggca gcaaaggcac agaggacaaa
     9721 cctcaaatgt caatagtcag actgatctct ttatcaaaat tccataggag gctgtctcac
     9781 aggggcagcc tctctttttg cacgtaagcc gcaaaacggt tctgctattc aatcgtaaca
     9841 tttctgaaac tattattggc attaaatgta ggaaagtaca tcaattccac ttttgcagga
     9901 tttaaagcgt attttcccgt ataccgtggc atcagatcaa tagtaaattc ataatcgccg
     9961 ggcatgagct tttcacagaa tattacagtt tcatttctaa acgattcccg gtatacctcc
    10021 caacgggaac tctttgattt attcaaatat gaacacccgg ccggaagagg aatattgacc
    10081 acaacataat cggcatcctt tctaacctta agctgtactc tcagttttac ttccttcccg
    10141 gcttttaata cattattgtc tgtatcaata aagcttgtag atatttcaaa gtccgatttc
    10201 ttctccagtg gatcaggatt ccaatatctc tggtaagcgg ttaaataaac cgggaaatct
    10261 ccggattttg ttatttcaag agtatcatcc gacgatacgg taacttcata agggaattca
    10321 tctgtttctt tatctatagc tccggatatg accagctttg gtttgatgac ctgttctttg
    10381 gaggtcaaca aatcaggaag aattgtctct attatcttag atgattcata agtattcagc
    10441 cagaacccgg ttgatctgtt ctcaaaaaag taattcctta ttttcaaaag agtccggtca
    10501 tgattgactg cggtatcacg gctgataatc ctgtaagcaa taattgcatt ctgaacatta
    10561 tttgtcaaca atcctgatgt gaccttgcca gaggcaaagt aaatattccc aaacattgtc
    10621 tctttcctaa acgatttcaa agtatccaga ctgactttaa ttccacagat ctgttttaac
    10681 tcgatcaatt gaaacaactg attcatagtt agttctccgg atttttctgc atctgatatg
    10741 agagcgggat agttgaccgt ggcgttcaga gtttttaaaa tctggatggc acttaattta
    10801 ctttgtataa gattggttct tgacatctcc catagaagat tatcagctat tttattttca
    10861 ttgagattaa ccctgtatcc catttctctt gcctttgtca aagcttcaag aacgtggaga
    10921 ctgatccaga gacttgactc tttagaactt ttccaccagc cccataattc atcttcctgt
    10981 ttgcttctgt ttaaaagtcg gatgattctt tctacctcat ttgacttttt aaaggcttta
    11041 tccttgaatt tgcaaataat ctgttctgat aacagagctt tcagttttga tgcgagctgt
    11101 tcattgcagg aataccggta agcaatcaat ttaattatct cttcctcaag aacctccagg
    11161 acatctgctc tggcatatac ttttgcatcc ccggaaatcg ggctgaaact gacattaatg
    11221 gtcgtatccc tgtcaagtac aaaaaactga ccggcagtct cttccaatcc cggcggaaat
    11281 attgggatgt gtttgtgctc cccgtcaaag taaccatcat ctttttcaag atagtacttt
    11341 actgatacag aatcatccgg acaagcaact atcggaaggg tatcaattat tgatcttatg
    11401 cagttttgtt ccttcacgga gattaagtta ccgtcaatct cgaatttggt tcttaacgga
    11461 atagtgtcgg gagtatagtt taaaaccttc cccaatgcga gggtactgtc acctgcaaca
    11521 agaaatccgg gaactgataa ttgacccatt aaaggtttat aggacttgat tacaccctct
    11581 gtctgcccgg attgcttttt cccgttcatg gccaggtaaa aggttttcca gcttgtcaca
    11641 tcatcaggaa atgtgacttc aaaagaggcc tttccttctt tgtcggttac cagttcaggt
    11701 tgccagaagg cataatcgga aaaattattc ctgatggagc ttgattgtga agcagcctcc
    11761 aaaaaggctt catcatactc cgctcccaac tgtccgggag attttgttgc aagaaatatt
    11821 ccccctgtgt ttatcaatac aacgccatta gccccacgtg caccatatat agctgtagcc
    11881 tgagaatctt tcaggatttg aatccctttt atcaggtcag tatcgagttc attaatattt
    11941 cctgtaaaaa ccattccatc aataacatat agtggctctc cgttaaaatt cgtcgtgcca
    12001 cttcccctga tatgaattga gaccacacta tcaggaacat cagacctggt tatttcaact
    12061 ccggcaacct tacctgccaa agtgcctgtc agggctgatt ccataccacc aggtattccg
    12121 gctgtattaa caactgaaac agatccggtt aatgagcttt tttcccggac cccataacca
    12181 ataacaacta cctcctgaag attcaatgct tcgggtgtta gattaacatt cacggttttc
    12241 ttatccgaga tgtcaatctc ttcctgttga tagccaataa atgagaatgt aagagtattt
    12301 ttttccctcg ggacattcag tgagtaataa ccatcgagat tactgattgt gccatacgtt
    12361 gttcctttaa ctaaaacagt aaccccgggt attggatttc cttcttcatc gtggatgtag
    12421 ccttcaataa tatcccctgc acccgtaaaa cggaactctt gctgatacct gtcatagatt
    12481 tgctttattt ctctctcctg ccttattggg tttgacacag gcttaaagat attctcctca
    12541 ataatcttac ttaccgaagt actaaatgca tctttaatat gtttttcagg caattcaatt
    12601 ctatggtaat taagtccatt cggggtaaca aataatgaat caataacaga atactcagat
    12661 ccggggagaa agaataaaac tttgtaatat cccttatcga ggtcattaaa tgtaaattca
    12721 cttccagggt atattctcag aaactcactt tcgtcatacc tgaataataa aatattcagt
    12781 ggccttaatg atgttttgtt accatcagcc agcagatcga ttttcattgt acccattcct
    12841 gcggatgttg tcccggggta gttatacctg gcagttaagt acctcttttg attgatgtag
    12901 tcattccagc ccttattaat atcttctgca tttaaaacct tgtcggccat actttcacga
    12961 gcaggataat tatacaaata accgggatac ctgaatttgg gatcaatgtt tctcattttc
    13021 aataatcccg gcgcgaaatc atattcaaaa aaaggttcat gggtgaatcc taccgaaaag
    13081 ctatccacca gctgaaattt tgtgcctgtc ggatcaacag gaccagccag attgtaattt
    13141 gggcctgatt tctccggttt cagaaaaaat actctgctgt tctgttctat gtaaccatag
    13201 tattcaccaa actgatatct gtatgggaaa atgtatttat acagaatagc tttctcataa
    13261 tcactcaaga gaggtttaac cttgttgact tgtacatttt tattgataat gctgtctctg
    13321 aggcttaaaa tagttttagt gtgatagttg aaatatacac tgtcaatctc aaaatctctt
    13381 tgggttgtcc tgagctttac atgatggtag ccgctgtcaa cagagaaaga atagggctgg
    13441 atatttgttg accagctgaa gtatattggc ttcctgtcta cataaataac atgaattggt
    13501 accggttgac cacctgaaat cacaaatggc gcaaactggg ttgtttctgt atcatattga
    13561 aacctgtaaa tcttgttccc gggatagata aagttgtaat attcaatcgt atccaggcca
    13621 gcccataatc gccagtagtt ataatcaaga ttcagtccct ggaaataatc aaatagatgt
    13681 tcatttatcg aaaagttatt tatgatactc ttgtttttcc tctgagttcc gagatatggc
    13741 aatgagggtg gagtgtagtc aaatttcccg gtaattgaat atgcagttaa gtcaacatcc
    13801 gggacaggat ttccttccgg gtcagtaact aaaatctcga tggcagatgt ttgagaggga
    13861 tagacaattt ttggctcgtt aaccgtaatg ttaagctttt tatcgtggta tgggatttta
    13921 taattctcat caaccaactt tcctccccac aggtactgaa tagccagaaa atagttctcc
    13981 ttgcctgaag tgaggatatg caaattcaac gaatcagaat gtcctcttgc cttctcactg
    14041 ttctttttgt aaatgaagta actgaaagga agtttccttg gattatttac cataatccgg
    14101 atgctgtccc ttgatctttc tgaaaagcat tgcagcattg aaggctcagt tgataaactc
    14161 acttttgttt taacagaatc accatctgcg caataagcag aataatatgg gtttattccc
    14221 gtcttcagag gcagccaacc ctccataatt gtagtttcgt tgccaaaatt gtcaatgccc
    14281 agaatccttg ctctgaccgg actcctgact ccatttttct gataaaagaa ttctatcgaa
    14341 tcattagtca ggtcgtaacg gatttccgat ttagaaaaga gataggttaa gtgttctgtt
    14401 tttgtgacag tctcattatc gctgtttaaa agagtgacca cgacgtcata ggacagattg
    14461 gcgcgcggaa aaattgaatc cgggacagtt atacttgttt cattctcttt ttcaagatcc
    14521 agttgccagt aagcgagagt gtcggggata aaaacataac ttccaaagta tttctgaact
    14581 tcctccggtt taaccaggac ctcaagtctg ccatcaagta tattcagccc attttcatca
    14641 gttcctttta ccctgacaat tacattttta ccatgaaaat gttctgttgt atcagcgatt
    14701 aattttaatt gaatgcttct aagctcatag tcttcatatt tgaatgtccc tgagatatat
    14761 tccctttcct tattgttttc aaatgaaact ctgtaattct ggtcaagcag caaattcaat
    14821 gaatcatgca ggtaaaattc ataagaatat ccgccttgct tatacggcat taatgtggtt
    14881 aaggtaacat gctttcgggg accctttaag acgactttaa catcatcctt taccgctctt
    14941 ccgcttttat taactatgta tgcctttatc tttactgtat cgcggggcat atattttggt
    15001 ttgttgaaaa ccatataccc tttataattg ctgccagtgc tgtagtaatc acaataagaa
    15061 tcatcaaaca gacacatgat tttataaaat gacttcctga aaaaccagga ggttctctgt
    15121 attgtcccct gagggtaacc atgagatatt gaccttaccc cgtcaattgg cagaaaaaca
    15181 atatacctta ccggatacca tacatatttg acaggggtgc cataaactat ttttcttgca
    15241 gttcttttaa tagatgaatt attatactct cttttaagat catagaatgc agtaaatcca
    15301 ttatgattta ctttcaataa tcccttttga tttgattttc tatcaagata ggattgggtt
    15361 ttactatcaa agcggataga ttttagccgg acagacacat ccgcatccgg aattatttcc
    15421 ccttttagat tatatacctg tacagcaaga tctgtgttgt tgtttaaaat cattacatcg
    15481 aaatcctgca cagaggttat gtcaatcctg agtctgttct tttcagtata gaccctcaga
    15541 taatgaccaa tgggaaggtt tttcacaaat gcactgtcag tgggaaaaga atcaacaagt
    15601 gtatggaaga atttttgatc aacctcccac agattatttt tgtatatctg ctccgcctcc
    15661 ctgtcattta tcttgtatat gtaagtataa aaacttgatt gcctgctatg cagaaggtct
    15721 tgaccataag tcacgccaga gagcattggt aacaccagaa gtggtaaata tctgagcagg
    15781 gatcttaaaa agaaagtaga tttcataccc gcagataagg ttataactat ttaacattaa
    15841 agataatgaa tataaggcat ctgcaatagt ttcgcgactc ttttatgtaa aatcataaag
    15901 acacggttat tattctctgt gaccacttca aaagaagacc tgaaaccatt aagtatttat
    15961 aaacattcca cttacctgca gagtctggca gaaactggca gaaactgaca gaaactggca
    16021 gaaactgaca gaaactgaca gagactggca gagactggca gaaactggca gaaaccaggc
    16081 aaaaaaagaa acacccacac atcatccgcg caagtgtctc attttcagtg tagcgagacc
    16141 gggaattgaa cccgggacct catgattatg aatcatgcgc tctaaccatc tgagctatct
    16201 cgcctgattg atattttaat gaactcccgg ccttcccggc ctgccgccgg atgatctgac
    16261 catccgaacc tgctcaccag aaaaggcggg tttttcaagg ctgcaaatat aaaaaatttt
    16321 tcatttatgc ctgtcaaact tttgtcttct catctattat ttttatattg catgacactc
    16381 atcccccaaa atgagaacca aatttgaaat cgaattcacc ctcaatacac ccgtaagagt
    16441 tctttacgac aggctgagca cccccgaagg actgtcagag tggtttgctg acaatgttac
    16501 tgtcgacggc gatatcttta cattccactg gaacaaatca gaggcaaaag ccagacttgt
    16561 ctacgccagg gaaaataaag ccgtcagatt cgactggctc gacgtcaccg acaccgaaac
    16621 aaacttcttc gaattcagaa tcattgtcca tgaacttacc aatgataccg cactcattat
    16681 aacagaccat gcggaagagg atgagataga agattcccgc gaactctgga acaccgaggt
    16741 ctcctacctg aaaaaaatcc tcggaatcac cgagtgagag agcgttgcat ctttcttaaa
    16801 aataacttac gtttgcacca cacccgagac gatgaagaaa atcgatctgt ttatgataag
    16861 atcgtttata ggaccactgg tcctgacgtt ctttattgtg ctcattattt tcatactcca
    16921 gttcctgtgg atgtacgtcg atgacctcgc cggaaagggc ctggagctca aagtcctcgc
    16981 tgaacttctc ttccagttct cactcacatt cgttccgctg tcactcccgc tggcaatact
    17041 ccttgcctca ctgatgacat tcggtaacct cggcgagttc ctcgagctga cagcccttaa
    17101 atcttccggc gtgccgctcc agagaatcat gatgcccatc ttcatcttct cttccctcct
    17161 tgctatagga tcattcctgt tcgctgatta catacttccc atctcaacac gaaaggcaag
    17221 gacactgctg actgacattc gccgcatgcg gcctgaactc aatatccagg caggaacatt
    17281 ctataatggc atcgacggct tcagcatcaa gataaccgac aaggaccctg agactaaccg
    17341 cctcgacggg atctatgtct atgaccacag ggacggagta ggaaacagtt cggtcatata
    17401 tgccgattcc ggttatatga atgtgacacc cgatgagagc ggcctcatac tgactctgta
    17461 taacggacat gcatataagg atatagaaga gaaagacgtt cagcaggata aaagaaaata
    17521 tccattcagg cgtgacaggt tctcagaaca gacaatggtg gtggaactgg aaggttttgg
    17581 cctgaaccgg tcaggcatgg atatctaccg ctcaaactac gcgatgctga ataccgctga
    17641 cctcacatac ttcattgact cactctccac catgctcagg tcaagggccg atgcctacta
    17701 ctcggagttc aaccagacaa ggctccttac aaccccgcac tttagattgc ggggcataaa
    17761 cagggaagaa gattccctgg cagcatggaa gacacagaag ttcgttgcac gtgccgcttt
    17821 cgacacactg agcttcaacg acaagggtac caccatcagc agagccctga gcttcgcccg
    17881 cgagggtgcc tcattcatga tagaaaagag cgagtctatc gtggcggaag caaagaacct
    17941 ccgcaaatac gaggctgaaa ggtacaagag atttaccctt ccctttgcat gcctggtatt
    18001 cttctttatc ggggcacccc tgggagccat tatccggaaa ggcgggctgg gaaccccggc
    18061 agtgatctcc gtgctcttct tcgtaattta ctatgtaatc accctctccg gcgagaagtt
    18121 tgccaaggaa ctgattataa acgtacacct gggcatgctt gcctccacca ttatccttct
    18181 ccccataggc gcgttcctga cctataaggc taccaccgat gcagccataa tgaatgccga
    18241 aacttatatc gccttcttca ggaagatagt agccttcctt accggcatca agcccgaaaa
    18301 agatgatgaa ggtactggtg ctggcgccta ggcccctgtg gcctgcacat gacggaggaa
    18361 cagtggcatc agttcactgt gtcagcggac tggctgccgc cggagctgaa gtgacggcgc
    18421 tctcgatggt gacagaaaag catatgacct caggaattta cgcctgcggc gcgaaaccgg
    18481 attatctcac tgattacatt actgttcagg taaatacagc gatcagacct ctcccgatga
    18541 tcctgaacct gctcttctcc ggcgaacctt atgatctggc aaggttcagg tcaggggaat
    18601 actccaaagc acttcgcaga atacttaccg gaaatcagtt cgacatcatc cattgcgagg
    18661 gtctgcccct tgtcctctac ctgaacggga tcagaagcct gacaactacc cctgtggtgc
    18721 tcagggctca taaccttgaa cacaccataa gggagatgat ggccgccaca gcctgttgtc
    18781 cgctccgcaa atattacctg tcactcctct cacgaaggct gaagaagacg gaagcaaaga
    18841 cagctaggca gtttgacgcc gtggtgccca tcagcgagcc tgacgccgcc tggttcaggt
    18901 caatatccgg ggataaaccg gtgttccttg cagaggcagg aacagacgaa gctcttatgc
    18961 tcccggagcc tccacaggaa aacccgcgcg ttggattcat cggtgcaatg aactggcaac
    19021 caaatgcaga cgcaataaaa tggtttattt ccgaagtgtg gtccggggta atcagaagga
    19081 ttccttcggc aaccctgcat gttgcaggaa gaggactccg tcagactgac gtcctgcccc
    19141 cggggcccaa catcattaac gtgggagaac ccgacaatgc ccgtgaattc atcgcctcaa
    19201 accatgtgat gatagcgccg ctctttgccg gaagcggtat caggattaaa atcattgagg
    19261 caatgagcgc cggaaggcct gtggttgcca cccctgtagc cgtggccgga ctgcctgtgg
    19321 aaaatggccg ggaactggca gttgcctctg acccggcaac attcaccgct gccattgtca
    19381 ggctgcttga agagccggaa ctgagagcct caacaggaga gagagcctta aggctggtaa
    19441 aggagagata tgacaacacc gtcattaccg cacggctgct tgaattctac aaaaaactga
    19501 gacatggcag ctgaaattat tctggctata acagctgcat tgctggcatg gacctatctc
    19561 atctatccgg cagcagtaat catggcagca aacctgaaga agaggcatac cctgccctca
    19621 gccgttgcct tagaatcact tccatcagta accgtggtga tgtcgctgca taacgagaaa
    19681 gaagtggcag agaaaaagat tcgcagcatg ctggcctcag aatatccggc cgaaaagctg
    19741 gacttcatta ttggttctga cggttctgac gacggcaccg gcgccgttat aaccgctatg
    19801 gcagccacta acccgaatat tcagatcatc atcaacgctg aaagaaaagg aaaggcagcc
    19861 atgctcaacg aactcgtatc aggggcaaaa ggagatatac tggttgtaac tgatgccaat
    19921 gtgatgttca ccccttctgt tatcagaaaa ctggcggagg gatttgctga ctcccgtacc
    19981 ggattatgtg atgccaccgt tcatccgttt tctgctgatg atacgggaat aacgcggcag
    20041 gagatcatgt acagccggtt tgaaacagcg ctgaagaggg ctgagggctt gctctgggga
    20101 gcaatgcccg gaccgtacgg aggatgctat gccgtgcgcc gcagcctctt cccccggctg
    20161 ccagaaaaca ccctggttga tgatcttttt gtgggtttat cagtgctgaa agagaactac
    20221 agggctttca acatccccga tgcagtggtc tttgaagata cccagcctga catgatagaa
    20281 cagtaccgcc gaagaacaag gattgctgcc ggatcattcc agaatctttt ccgtttcgga
    20341 ccctgcatct cagaaagagc gggcgctctc tttgcttttc tttctcataa gataatgagg
    20401 tggttaacac ccctgctgct ggtgctgttt ttcatgacaa cggtcattct ttcaggcagc
    20461 tcttatttct atttttgtct tacagcattt cagctgatat tcataatttt gtcagtgatt
    20521 gacatcatgc ttgacaggag gggcaaaaag ttgtttattc agcgttatgt gacccagttc
    20581 ctgttgatga atgcagcatt ggcaacaggg tttgtgaagg cagtaagagg gattgaaagc
    20641 gggatatggg aacccaccaa acgtgtttga aatgacagat gatacaaagc tgaatacaat
    20701 taatgaagcc atcgaagaga ttcgggaagg ccgggtggtt attgtcgtgg atgatgaaga
    20761 cagggagaac gaaggtgatt tcatctgtgc ggccgagctg atcacaccgg agatagtgaa
    20821 cttcatggca acccacggac gcggtctgat ctgcgtacct cttcccgaag agagatgtga
    20881 ggagatggag cttgacatga tggtaggcca caatacatct cttcaggaaa cacctttcac
    20941 agtgtcggtt gaccttatca gtcccgagac aaccacaggc atatcagcca aagacagggc
    21001 acttacaatc aaggcgctgg tagacccatc cacaaaaccc ggccagcttg gaagacccgg
    21061 gcatattttc cccctcaagg cccgttcaaa aggtgtactc cgcagggcag ggcacactga
    21121 agctgccgtt gacctgacca ggctcgccgg gctgaacccg ggcggtgcac tggtggagat
    21181 aatgaacgag gacggaacca tggcacgcct gcctgacctg atcagactgt ctgaaaagtt
    21241 cggcatcaag ataatatcta tcaaagacct catacagtac cgcctcggac ttgaatcgat
    21301 agtggaaatg ggggaacggg tcaaactgcc caccgagtat ggcaacttcg actttatccc
    21361 tttcaggcag gtaagcaacg gtctcgagca tgccgcactg gtgaaaggac attggacaaa
    21421 ggaggagaca gtactggtac gcgttcactc ttcctgcttt accggtgata tatttgcatc
    21481 caagaggtgt gactgcgggc aacagcttca ccgggcgatg cagatggtgg aagctgcagg
    21541 gaaaggggcc atagtatatc tcaaccagga aggaagaggt atcggcctga ttaacaaaat
    21601 acgcacttat aaactgcagg agcagggtcg tgacactgtg gaagccaata ttgaactggg
    21661 attccgcgag gatgaacgtg actacggtat cggggccaat attatgaggg acatagggct
    21721 tggaaaggta aagcttatct caaacaatcc ggtaaagagg gcaggactgg aagggtacgg
    21781 tatctcaata gtggagacca taccccttga aatcactcca aacaagtaca actcgtttta
    21841 cctggagacc aaggccagaa agatgggcca cagcattaca tgctacaaga cccgggcaaa
    21901 aggcaaaaat gactgaacca acgctgcgca tagtatttat gggaacgccg gaatttgccg
    21961 ttgcgagcct gaatgcactg tatgaaacag gggttgacat agctgcagtg gttactgctc
    22021 cggacaggcc ttgcggacgc ggccgcaaga ttaccttcag cccggtgaaa aagtatgcca
    22081 tggatcatag gcttacactc tttcagcctg agaaactgcg tgacccggaa ttcatttcag
    22141 ccctgagaga tattaatgct gatctgtttg tggttgtggc cttccggatg cttccggagg
    22201 aggtctggag catgccccgg ctcggcacat tcaacctgca tgcctcgctg cttccgcagt
    22261 acaggggtgc agcgcccata aaccatgcca tcatcaatgg agagaccatg acaggcgtta
    22321 ccacattcct cattgatgaa gatatagata cgggtatgat cctgctctcg cgcgaaacgg
    22381 caatattacc tgacgagacc gcaggcagcc tgcatgaccg cctcatggag acgggcgcgc
    22441 gcctggtggt tgaaactgcc aggggactgg ctgccggaag cctcaaaccg gcaccccagg
    22501 aaaagatgga tgacacaatg ctgaagccgg cgccaaagat atctcctgcc gatatggtta
    22561 tcagatggga aaagatagca gaaaacattc ataacctgat aaggggcctt tcaccctgtc
    22621 cgggtgcaaa caccatgctc agaaaggagg gcagaagcct caggctgaag attctggaca
    22681 gcaccatcaa ctatgagatc accggcaata tacccggaac gcttatcgtg acggagaaat
    22741 cgaggctgat ggttgcctgt ggcaaggaca ccctcgagat actttccctc atccctgagg
    22801 gacgaaagag gatgagcgct gcggagtttc tccggggttt cgatttatca ggatgggaaa
    22861 tggcctgaca ccggctatct taacctcagt ttctgccccg ggcggcattc ggtaccctca
    22921 tccatcaggt tcatcttata gagcgacgaa agtctgatgg catacttctg tgagatgctg
    22981 tgcatggtct cagcagcctc aaccgtatga accagtaacc cctgggctgc cctggtgcgc
    23041 ttgggcttaa tatagagcac ctgtccgggt atcaatggtg caccggcggt gagatcgttg
    23101 aagcggacta tctcccatga gagaagctga tggcgctcag cgagcgattc ataggtgtct
    23161 ccctcttcta caatgatgta ttccacattg ttgttctcaa gcgtcttcgc tcctgttgag
    23221 atgctgatta ccctgacagg ctcatcatct tcgattgcgg ttgccctttt gtcaggcacc
    23281 ggctgagcct gtcccgtacc ggtctccttc acggccggac ggggtgtaac ggtggttcct
    23341 gatgaggttg ccgctgcagg caccgtacca cttcccgccg gttgtgaacc tgctgcctgc
    23401 tgtgaaacta ttggcgaggt tcctcctgta tcatatgccc acagactgta atcttcgatc
    23461 ttgcggataa gcagttcggg atacttcgga tcggttgcat aaccagcttt cttcagcccg
    23521 tgcgcccaac ccttgtaatc cgtggctgcc aggtgaaaaa ggttgcggta acgcgaacca
    23581 ttcaccagga aatcagaatg gtccctgtat gactcttcgg gtgaggcgta agacctgaaa
    23641 cactctccct tgcgattgtc atcgtgatat attctttttc ccgaccagtc actgtgacac
    23701 ttgatgccaa aatgattgtt gcccagcgtg gccagccggc tgcgtccgta gttcgactcc
    23761 agcatgccct gagccagggt gatgctggca ggaactcctg atcgtttcat ctcgctgaca
    23821 gccagattgc cgtatttgat aagatactcc ctcgcataat ccgtagccgg agctgtagag
    23881 gccgtgcttc tggctgtcct tcgggccgga ctgcaggaat tgaacaacag gagagatatc
    23941 aggaccagat atataaacag gtttttcatc cgaagccttt tggcaaaaat aagaaaatgt
    24001 cagcagcccc gacaaacagt gcggcacaag ggcaaaatat tattactttt gcggcactgt
    24061 aactcattgg taatgtctat gaaaaagaga gaatcaacta tctctttcct tgaatacaac
    24121 agcgaggatg agttgccggc aatggaaaag gagcttgttg gagaggctgt cagggcagca
    24181 tcgggagctt attcccccta ttcgcacttc atggttggag cagccctgct tcttgacagt
    24241 ggagagattg tgaagggtgc caatgttgaa aatgccgcct acccgtcagg aagctgcgcc
    24301 gagaggactg cactgtcata ttcggttgcc aatttcagtg acaggaaagt ggtggcgata
    24361 gcagtcgctg ccctgaccga tggcgtactg actgaggacc cggtaacacc ctgcggcaac
    24421 tgccggcaga tgctgataga ggaagaacag aggaatggaa gaccgataaa ggttattctg
    24481 gcaggaaaat cagccattta tgtccttgaa aacagccagg ggctgatgcc gctgagcttc
    24541 acggccagca atctcagcaa ataaccggag gatatttgcc tcctgtctgc tacctattgt
    24601 ctgcggtctc ctgcctcctg cctgttgcct attgcctcct gtctgctgcc accctgcctg
    24661 ccgtcggcta ccttcccaga ttattccagg ataaccagct gaaacccgct gccatgaatg
    24721 ttcttaagga ggatattcgg atcatcactc aggtatttcc tgagcttggt gacataaacg
    24781 tccatactcc gggcagtgaa ataatcatcg gagccccata tcttcctcag ggttgcctcc
    24841 ctgctgacca gggtattggg tgatatggca agcatctcaa gcaattgccc ctccttgggc
    24901 gataactgtc gctccctgtc gcccagggta agagtgcgta accgtgaatt gaaaacatat
    24961 ttcccgatct catatatgtc gcgcgttccg tccttaatat ccctcctcgc aatgatagcc
    25021 ctgatcttgc acagcaggat gtcagtgtca aacggctttg taatataatc gtcggctccc
    25081 actccataac ctttcaacag atcctccttc agcttcttcg cagtaaggaa gataatgggt
    25141 atgaccgaat tcatgatcct gatctccgaa gcaatcgtga aaccatctac atgcggcagc
    25201 ataacatcaa gtatgcagag atcatacact cccttgcgga aggtctccac tgccaccttc
    25261 ccgtcatcaa cgaggtcaac ctcgaaatca tttatctcaa gatatgattt cagaaccgat
    25321 ccgaaacttg catcatcctc taccagaaat atccttgcgt ttttcctgct catgcccttg
    25381 tttcattgct gaatggtaaa aatattgtaa aaacacttcc ttccccctgt tcactttcta
    25441 cagtgatagt tccaccgtgg gcttcggcaa tggcttttac atagttaagc cccagcccga
    25501 agcccttgac attgtgaata ttgcctgtag tttcacggta aaaccgttca aagacccggt
    25561 tctgcactga cttgctcatc cctatgcccc tgtcagccac ctgaaggaaa atcccgctgt
    25621 cattgctgcg ggttttcact gtaatgtcag gcgatttttc ggagtatttg tttgcgttgt
    25681 caagcaggtt atgcaccagg ttgcgcaggt gttcggtgtc accgttaata atactccttt
    25741 ccgcatcgag atgccggtga accacacctc ccctgtcggc cacctggatc tcaatggtgt
    25801 ctatggcctg acagatcacc tcatgcagat caaccggggc aaatctgaaa tccatctcgc
    25861 gcttgtcaag cgtggctatc tgaaggatag actccacctg cctgttcatc cgcaagttct
    25921 ccttcttgat catactgatg aaatgacgaa ccctgttctc atcagttata acccggctgt
    25981 ttgcaatggt atccgctgca agtgagatgg tggctatcgg cgttttaaac tcatgcgtca
    26041 tgttgttgat gaagtcggcc tgcatctctg aagtcttctt ctgcctgata atgaaccaaa
    26101 ggctcagagc aaaggttgag aggattatca gcaggaaaag gccaaatccg ccaagcataa
    26161 gtcccatggt tccaagtaca tagttggtct tcctggggaa gaccaccgaa agtgtttctc
    26221 ctcttctgaa cagcccctgg gtaaagaggc tgaccttgta cgggctatca agaaaatctt
    26281 ttccttttac cttgtcgaag actccgtcaa ttatggaatc accccggatg attgcaaact
    26341 caaacggcgt attgatgcct gaggcagcca gttcgccctt cagggtctga agcagctcct
    26401 ccttgtttat ctccttgttt ctttcccaga cgaacatctc ggatgccagc tggttgctca
    26461 tcaactggaa ctcgtttgcc tgctgctgca tccaacgcat atattcatcg gaacccacaa
    26521 caatgctgct gcccattttt tcactggcct cctgccgggt aaccttcatc ctgaccggcg
    26581 ggttctcatt ctccgagacg attatttcca ctgaatcact gtcggaattg atctggctcc
    26641 ggaaagagta attaccggtc atgtgactct gtaatgctgt tcgctcaatt gtctgaggaa
    26701 gcgatgaata tcccctgatg aacatctcat tgagaaaatt catcctgcgg ttcgtctcca
    26761 tagagtgcgc agtgttgcgg agagtgttga tcacgaagaa atcaaactgc tcgttctggg
    26821 ccctgacaga attactgatc catgtcacct gaacccatac gatagccacg acagatacta
    26881 ccatcagcac tatcagacct gtgaatttcc tcatgttcat ggcacaaaat tatacatata
    26941 aatatagttg aaacctgttt taactttttg ttaaccagat ttaacctcac tttaacccct
    27001 ctcaggatta tctgaactat ttttgtaatg tcagaaaaac aaacaccaca gatgacagac
    27061 aaaagcgctc tcaacggtta catgaatagt ggcagtcatc tttgcggaac aataacactc
    27121 tccgcacagg agattgagaa tgtcaggatg aaggcttaca aaaaaccgga gcaacatctt
    27181 taactggcat ttgcacacaa aagatcagga attatagaga ggaaggttgt tgattcaggt
    27241 tagtttcagg ttaattggaa gaggcgcggc agaggcgcct cttcttcttt tatatgtcag
    27301 ggcatgtttt cagacggggt acggaggtac agcccgccat atcagctgta atcatacatt
    27361 tttaaagagc ttccagatgc ggtcatcagg cagaggtgcc acaacgttta tctcctcatt
    27421 cctgaccggg tgaatgaaag agacccgcct ggcatgaaga gatataccgc caccctcgtt
    27481 ggaacgcctt gaaccgtatt tcaggtctcc cctgacaggg caccccgcag cagccagctg
    27541 cgccctgatc tgatgatgcc tgccggtaag aagatcgatc tcaagaaggt aatatctctc
    27601 tgaacgagcc agtatcctat acgacagctc tgcactttta gagcctttaa cctccttgtc
    27661 atacacatat gatttgttct gcttctcatt cttcttcaga tagttctgaa gcgaaccctc
    27721 ttcggcaggc ggccggtctg tgacaattgc ccagtaactc ttctttacat cacgtgtcct
    27781 gaaaagctcg ctgaggcggc tgagagcctt cgaggtgcgg gcgtagacaa cacagccgct
    27841 gacaggcctg tcaaggcggt gtaccacccc caggaacaca tttccgggct tgccgtactt
    27901 ctccttaatg tatctcctga taatattatc cagtgactca tctcccgtgc cgtcaccctg
    27961 cacaaggtcc gatgaccgct tgttgactat gattatgtga ttgtcttcgt aaagtacttc
    28021 cggaaattcc ccgctcatct gattattcct gacagcttaa aaatcccaaa gacttcaaaa
    28081 cactccgcaa atcctctaat actgttcctt ctcgttcgga aaactgcctg acctcacatc
    28141 agcgatataa tgttgcaccg cccccttgat ctcatcgtaa agattatggt atctcctcag
    28201 gaatctcgg
//
LOCUS       DAOPBK010000006        27987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_1858_length_27987_cov_5.328942, whole genome shotgun sequence.
ACCESSION   DAOPBK010000006 DAOPBK010000000
VERSION     DAOPBK010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27987
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1858_length_27987_cov_5.328942"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /gene="cobS"
                     /locus_tag="PLA17_00895"
     CDS             <1..771
                     /gene="cobS"
                     /locus_tag="PLA17_00895"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HPK83998.1"
                     /translation="KEFNLFLNALMFFTRIPCPRVTEYSEAIMSKTLRYYPLTGFIVA
                     AITAGVLWLALLVMPVPVAVTIALTVSALVTGAFHEDGLADFVDGFGGGWSRDRILEI
                     MKDSRIGTFGTIALILLFALKIVALSGLPVEKLPAIIIAAYCVSRVFPSLLVKTSVYA
                     RKNEGKAFFSRNGIDWKSIVIISVTGFIPMILFNYRFVILYTALSLAFLAAFRHYLHR
                     RIGGYTGDTLGAVQQITELIFYLLLTAVPASWMNSSVL"
     gene            747..1319
                     /gene="cobC"
                     /locus_tag="PLA17_00900"
     CDS             747..1319
                     /gene="cobC"
                     /locus_tag="PLA17_00900"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HPK83999.1"
                     /translation="MDEFLCLVRHTRTAMSEGICYGQTDIDVADTFCSEYPAVVSQLA
                     GIEFDLIYSSPLKRCLRLAELFSNDILPDSRLLEVNFGDWEGVRWDDIFGSPDGKKWF
                     SDYTSMRCPGGESFIDLQQRAWSFLQDISGKGKRILAVTHAGLMRAMLVSLGLEEADT
                     VFGKKIPFGGVVMLEKTQQGYICRTKLEPR"
     gene            complement(1376..1933)
                     /gene="cobO"
                     /locus_tag="PLA17_00905"
     CDS             complement(1376..1933)
                     /gene="cobO"
                     /locus_tag="PLA17_00905"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPK84000.1"
                     /translation="MEEKHFNGIRGTKPSRGYIQVYTGNGKGKTTAALGLALRAAGAG
                     KKVFFAQFVKGLPYSETKIIERCLPGIVLRQYGRGCFIHGKPGEKDIEAAAAGMEEIS
                     EIVRSGVYDIVVLDEASIAVYFNLITPDMLIELLKSKPEQTEIIVTGRYAPQELIDMA
                     DLVTEMKEIKHYYNEGVEAREGIEY"
     gene            complement(1914..2570)
                     /locus_tag="PLA17_00910"
     CDS             complement(1914..2570)
                     /locus_tag="PLA17_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPK84001.1"
                     /translation="MRIGGIVHSSLIDWEGKIASVIFTKGCNFRCGYCHNPYLVLPHL
                     IRKTRDIPVEEVLGDLEERKEWIDGVVVTGGEPTIHADLPDFIGHLNAMGLLIKLDTN
                     GTNPEMLRYLIVNKLLDYVAMDIKTVIEVSQYQEITGCSVNGITSLITESVEILRSSG
                     ISYQLRTTVIPGYHTDEILEQLNRELAADYYVTQPFREGENICFYTDMSKICNGRKAF
                     "
     gene            complement(2561..4771)
                     /locus_tag="PLA17_00915"
     CDS             complement(2561..4771)
                     /locus_tag="PLA17_00915"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPK84002.1"
                     /translation="MNGRPDTIRQIIKRNGDLVEFNPQKIEHAIFRAMRATGVPDRKK
                     AGELSAKVVEQLETEKVEIPTVEQVQDIVEGILFQSGDFRLVKAYILYRKQRELVRNT
                     KDIFSNIEVVDDYLSLKDWRVKESANSSYSLQGLNQHISTVITSQYWLNKLYPENIAA
                     MHRQGYFHIHDLGFLSVYCVGWDLRDLLVSGFRGVTGKTQTRPARHFRTALGQIVNFF
                     YTMQGEAAGAQAFSNFDTLLAPFIRFDNLGYSQVKQSLQEFLYNMNIPTRVGFQTPFT
                     NVTLDLVVPDFLKNEPVVYGGELHESVYGDYQHEINLFNKAFAELMSEGDATGTPFSF
                     PIPTYNITKDFDWDNPDYKPIWEMTSKYGIPYFSNFVNSDLSPDDVRSMCCRLRLDKR
                     ELNRRGGGLFASNPLTGSVGVVTINLPKLGYEASSEQQLMQRLRNLMELAKSSLEIKR
                     KTIESYTEKGLYPYSKYYLRDIRKRYNCFWKNHFSTIGIVGMNECCLNFLGVGIADPD
                     GKAFAIRVLNYMRDVLVEFQEETGNIYNLEATPAESTAYRFARIDTMQYEDIITANHK
                     DYLNGSKPYYTNSTHLPVNFSDDVFRVLNHQDELQTLYTGGTVLHIFTGEKAIPAASV
                     RSIVKKIAGQFRLPYFTLSPTFSICPSHGYIHGEHFECPECGSACEVFSRIVGYLRPV
                     SQWNDGKKKEFIDRKVFRFDNVAKKNSLYNRNENSDPVAVSAGDDGSKQPEGCA"
     regulatory      complement(4870..5050)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5381..6013
                     /locus_tag="PLA17_00920"
     CDS             5381..6013
                     /locus_tag="PLA17_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84003.1"
                     /translation="MYEAHKSNWYETLCFSQEVLHYRNDTVTATEIWHEAYKSPGNLI
                     IKFNDPDNGTGILFINDSIFSYRDGALESVRYYLHDLVVLGLDINTVLPETTAERALK
                     CNYDLNRLAEGQCMGRDAWIVGDTLSNCFWVDKSSHLFLKMRRRSGENSREVEFARYD
                     TVNGYPVATEIRFYDNEGALSMIEKYFNITPDCELSPAIFSPSEFNNAKW"
     gene            6485..7054
                     /locus_tag="PLA17_00925"
     CDS             6485..7054
                     /locus_tag="PLA17_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPK84004.1"
                     /translation="MKTKPLSLLLALLVLATGSALSQTTGNPVTDLLLSAWSPRNFTS
                     VPVTDQQIDMILQCGIKAPSARNAQPWHFTVITNEAVMKEIIPNITPGNLIIVISGKV
                     SQSGATPDFDCGLATQNMFVAATSMGLGARIYGAPVAAVRSKKEALQIPEEFSPVMMI
                     RVGNMEKSSDAISGASPRKTKEETVNWVR"
     gene            7287..10343
                     /locus_tag="PLA17_00930"
     CDS             7287..10343
                     /locus_tag="PLA17_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPK84005.1"
                     /translation="MKINRLFIVIVLILCSLVTSPGFAQDGDQMLDGIGETGLISRYQ
                     FKGNDRDWSRNNLHAITRGTDFRFIPDSLFGNVLALSGESETYVSIPGEAVSGEESLT
                     ICGWINLLSAQPGQMFFDFGKNSNSHFFAAPAGTKEKPGLQAAFVAGSKIYTTSSDAV
                     PLKKWSHLAVVLDIPAKTLSTYLNGALVAEVKDATLDLSQLFDTDSEKNNQLYVGKSI
                     DGEKSFLNAKLSDFRIYRIPLTSRQIGRIYFTALRGGSQQAAHRGQPEQTLQVFPATT
                     PQLYNAFLVSIPDTQVETTVGNLPRLPRFVKGVYSNGAEGSDVRVIWPAPEDNSQVLV
                     PGTYTVTGKVAGTSLQPRVVVTVRSAEAPATPNRTLEAFRLDQVRLDADIHNQETKFI
                     ENRDKFIKGLEATNPDNFLYMFRNAFGQEQPVGAQPLGGWDSQQTKLRGHATGHYLSA
                     IAQAYASTGYNAELRAKFAGKMDYMVKTLYELSQLSGQPRTAGGEYVADPTAVPPAAG
                     KTGYDSDLSEAGIRTDYQNWGKGFISAYPPDQFIMLEKGATYGGSNAQIWAPYYTLHK
                     IMAGLMDVYEVSGNGKALEIAKGMATWVYARLSRLPKETLISMWNRYIAGEFGGMNEA
                     LARLSRITGDDSFMKAAQLFDNIRLFYGDADHAHGLAKNVDMLRGLHANQHIPQIVGA
                     IEIYRGTGAPEYYNIADNFWDMVTHDYMYSIGGVAGARNPNNAECFTAQPASLYENGF
                     AAGGQNETCATYNMLKLTGSLFLFNQDAALMDYYERGLYNHILASVAEHNAGNTYHVP
                     LRPGSMKQFGNPNMNGFSCCNGTALESSTKLQNSIYFRSADNSALYVNLYVPSTLTWT
                     ERNITIKQATAYPKEDHTVLTIDGKGRFDLNVRVPHWATKGFFVRINGKDVKSEAVPG
                     SYLTLSRKWKKGDTVELRMPFRFYLEPVMDQQNIASLFWGPVLLAAQEPAPRTEWRKV
                     TLDASDISKSISGDPSKLEFMIDGVLYKPFFDTYGRHSVYLDVELK"
     gene            complement(10555..11076)
                     /locus_tag="PLA17_00935"
     CDS             complement(10555..11076)
                     /locus_tag="PLA17_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84006.1"
                     /translation="MKKISDKVLPSVLAVLMSAAILLSGCERYGERITAEVIDHTGCK
                     SLKSGSVDGTTPDTLSCINYVFEASSGTLQITHINAGFNCCPGKLTCHAELAGDTLFI
                     KENEQSAACSCNCLFDVNLTVRGLESREYIIQILEPYCGKQERLTAHVNLAETPAGSF
                     CATRKLYPWGIMR"
     gene            11403..12926
                     /locus_tag="PLA17_00940"
     CDS             11403..12926
                     /locus_tag="PLA17_00940"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HPK84007.1"
                     /translation="MIDLHHTGFDNEKYLREQTAAILERVNRFNNKLYIEFGGKVLFD
                     LHAARVLPGFDPNIKMRLLQMLKDKIDVILCIHAGDIERKKVRADFGITYDVDALKTI
                     DDFREWDIDVTAVVITRYTGQYPARVFRNKLEHRGVKVYTHSPTKGYPTDVELIVSDE
                     GYGANQYIKTTNPIVVVTGPGPGSGKLATCLCNLYHEYRRGVKAGYAKFETFPIWDLP
                     LDHKVNIAYEAATLDLKDEVLIDSYHLKRYGTNVVNYNRDIEAFHLLKRIFEKITGGE
                     SMYQSPTDMGVNRASAGIVNDDVIQKAAHQEIIRRYFRCAVEYTIGLADKEILEHAAK
                     IMEKVGAKPEDRKVVLPARKAMKDAEKGGKGNAGIFCGASIELADGTIITGKNSPLMH
                     AASSLILNATKYLAGLPDSLLLLPKNLIDSVTYMKKDILNGKEVSLDVEETLILLGIS
                     ALTNPAAQAALEQLTLLRGCEVHLTHIPTSGDEAGLRKLNVNVTCDPEYSSKSLFVS"
     gene            complement(13017..14006)
                     /locus_tag="PLA17_00945"
     CDS             complement(13017..14006)
                     /locus_tag="PLA17_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84008.1"
                     /translation="MKRSAFLLIIPLLFSSCNSPRLNFDKGIIPPVPANFSTMNSSRD
                     DYNSDLKISWADMTFSLIFSSNRNSNGDNFDFIGYHGNIIFDLIDGGFEMQAKAWESG
                     FLEAVNSDCNELGPYFISDYPYFHYYKKGEEERRFFYSSDPEGNLDIFCCRYEFANDG
                     FDPSGDPFEVTQLNTEFNEGYFSLHHGAAQSSETAYFMSDRDGSFDIFSATGEEGKLI
                     SESAAVTITRSSVLSSSADDKCPYIAGNVMVFTSDREGGFGGFDLWYSVYNGQTWTEP
                     VNMGNLINTEYDEYRPILVPGGESFINDLMVFSSNRPGGKGGFDLYWVGVPRR"
     gene            complement(14003..14698)
                     /locus_tag="PLA17_00950"
     CDS             complement(14003..14698)
                     /locus_tag="PLA17_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84009.1"
                     /translation="MRKVFAILALLVLMAATCQAQQKIRKSVFGANAGVSIPLGEFAL
                     KTFTYDAGFAAPGPNLGAEYLYYGKIFGFSSSIGYASFFFNGKAYQSEYDRTLGGYGI
                     NDVSAGNYQMIKFLAGFTLKIPEIKHTEVMLLFHLGYAACIHPDLRVTNSEYGVINSI
                     AKTNNGNLVADAGLKINYWLTKRYGVSIKGSVSTTEPSFSDPAGPNGYFDMLMNHASV
                     NVGFIMNLKISSL"
     assembly_gap    14822..14921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15110..15661
                     /locus_tag="PLA17_00955"
     CDS             15110..15661
                     /locus_tag="PLA17_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK84010.1"
                     /translation="MADIQTIYQETIKFAAEKHGAQKVKGTRIPYVVHLSNVTMEIFM
                     AARQTRGFDLGYALQVALLHDTIEDTGTTRQELEMVFGKDIADAVWAITGDPKLIKEY
                     RLSDSLAKIRQCPREVGAVKLADRITNLQAPPKSWSKSRISDYLFDAKHILMALQGSN
                     EYLETRLAAKIEEYGKYLNVENS"
     gene            15778..17673
                     /locus_tag="PLA17_00960"
     CDS             15778..17673
                     /locus_tag="PLA17_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPK84011.1"
                     /translation="MNKFAYLFSLCLFLSTLFLSGQAGSFSFALITDTHIGSGSADED
                     LRRTVEDINDIDSVVFVIVAGDVTEFGSDAELLLAKKILDRLEKPWYIIPGNHDTKWS
                     ESGANSFRIVFGDEAFVFNHGGWLFIGTNSGPNMRMGPGQIPRENIVWLDSVLSIPAN
                     RAMKIISVNHYPLDNGLNNWYELTDRLKQLDTRLALCGHGHSNSVLNFEGIPALMGRS
                     NLRANHPQGGYNIMTIVGDTLLTASVRHPGTGEETEAATGHSAVTMPVWASVRLERHD
                     FSADTTRWPRPDYSMNERYSQVREVWRLQEEADIGAGTLVTGKLALITTTAGEVKAIS
                     LRNGREKWSFPTGAKIYGTPVSDGKRVIAVSADGMARSLTLERGKLLWSYDTKQPVVA
                     SPVISGERLFIAGSSGHCHALDITSGSVLWTNGDIDGFVETIPLIYKEMLIFGTWNNR
                     LYALDIATGTIRWVWNTGYSNRMLSPAACVPVAVGDRLFVVAPDRKMACLDVNTGELL
                     WHSDLGGATVRESMGLSEDRSLVLAKTMDGRVIGVSASGNSPEIIWKSDFNIGYDIAP
                     GVITERDGIIFIPSDKGIVHAASRENGKLLWSHRISSCLINNIVPLKGNNLIVNSMDG
                     IVVRLGW"
     gene            18038..18622
                     /locus_tag="PLA17_00965"
     CDS             18038..18622
                     /locus_tag="PLA17_00965"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HPK84012.1"
                     /translation="MAGFIDEFLESYGPEVTKQMSSNFNVDQATVQKLIPQLAPLILA
                     GLKKQKDTRGGDERVDHILNKYGDSSVLNNIKDLVSSKARAKDVDPNLGGLLGDAGGV
                     QAAQALAKKMNIDPSTIMKMIPALAPLLLGALTKKRDTGGKGISGVGALLDADGDGSI
                     LDDVAGFLLKSGSSTRSGSGGLLGSIIGGITRRK"
     gene            18634..19179
                     /locus_tag="PLA17_00970"
     CDS             18634..19179
                     /locus_tag="PLA17_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK84013.1"
                     /translation="MKKPVLFLSVLLVLWIAGASYWYVCKIRCDCKKAEASSVEMQAA
                     PDTLAPSPEQLLLASVNEAKQYLSESGVGKGFFPTSSAAGDMSGISDEYLEKLKLVLD
                     NDPAARVEVTGHTDITGTTSFNKELGLKRAEFVKSYLVNAGIKAEQIMTSSKGPSEPV
                     ASNSTPEGRAANRRSEIKVII"
     gene            19185..19856
                     /locus_tag="PLA17_00975"
     CDS             19185..19856
                     /locus_tag="PLA17_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84014.1"
                     /translation="MAERFTADLLFIIIVLLVAALLGFLIGYYLERGRCRKRIAILDE
                     EIEGLKAKIRSLEEEKRALEEKLRQAEEEKNNLTAKINGLEEEKTKLRDDTRRMDDEI
                     TSLKLTIDRLEKEARELRGDHSEGSIATDTPLAAMAHEEFKPDDLEVVIGIGPKIASL
                     LINRGITTWKALAETSVAYIQEILDKDGGEDYRIHNPESWPQQARLLDEGRWDEFREL
                     REQLK"
     gene            20187..21713
                     /locus_tag="PLA17_00980"
     CDS             20187..21713
                     /locus_tag="PLA17_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPK84015.1"
                     /translation="MTFNESFFYRPRKYNTIELWKDVTPEKWNDAGWQRKNSIRSVDQ
                     LKKVVRLTDHQESEIRRTIVALRQEGKESLRITPYYASLMQEDPFNPLFFGERSQKRL
                     DPIFWQSVPTPANLMFPDAGVEGAMSEGSRSYGAAYQRYPNRVALFVAENTSCASYCV
                     HCQRAKTLDTTADVNNREVDRGLFYIGYNRNINEVLVTGGDALMTSFKRLEYILGELS
                     RIPHLRIIRIATRVPVVLPMLITDELLEMIRTASNRFNRGPEKYVYFMTHINHYQEIT
                     ADLAAAVKKITSHGFTIRNQTVLLNHVNDHYRTLAETFRRMFWIGVHPYYLLQCHKEK
                     GIVHFITPIQVGKIYIKHLQGWISGITVPRYAVNIEGGGGKVLLMPSGHDTMNLDTSI
                     DDKISESFANVYTWDHKKIVKYEALGRATREEYKMAVGIMDRFIGRKGVFVPRVIIVD
                     EKGNHLETTNRFRLPTFEKTKKAALLNYELYNGDMPLTNPVDTYSEIEAAFLRSGYAS
                     "
     gene            complement(22027..24180)
                     /locus_tag="PLA17_00985"
     CDS             complement(22027..24180)
                     /locus_tag="PLA17_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPK84016.1"
                     /translation="MDISELKAKHKLINRWHYKWTPIDKGYTDKTLYINVGTGEIKDK
                     PVPLVMKEKFIGGKGYGLRLLWDATRPDTRWDDPDNEIVIASGPVGGITQYSGAGKSL
                     VVTISPQTDSVMDSNVGGFFGPFLKFSGFDAIELQGKAANDVIVFINGVDHYVEVFEA
                     PAEAIDSHVLAEQLTEMFANDESDRKNIGIVSTGSAADNSLIGMLNFTFYDGRRKKIR
                     MKQAGRGGIGSVFRNKKIKAVVCKIPGVKGNLNNVVDLEAIQERGRRFNREMRELDDS
                     QCRMRQVGTAHLMEIMDDHDLLPTHNYKYGSHKDAPRIDSAVWKSFFTQNIPDGCWIG
                     CNMACAKAVDDYEITTGPYAGQKVIVDGPEYETAAGLGSNGGFFDPRYIIEANFYCDT
                     YGICTITWGTSLAFMQECYENGILNKERTGGLELKFGNIPDALELLHRVSRGEGFGLI
                     AGQGIRRMKKIFAEKGWGDPQFMQDIGMEVKGLEYSQYMSKESLAQQGGYALTNKGPQ
                     HDEAWLIFMDMVNNQIPTFEDKAEALHYFPMFRTWFGLVGLCKLPWNDVEPENNAQTD
                     EPAKVPEHVDNYVTIYKAVTGREFDKHRLIEDSERVYNFQRVFNIRRGYGRRKDDDQP
                     YRACGPVTVDEYLSRQERYDKQLKEKIGVDPEGMSIEEKMKVTREYREKQYDLLRDAV
                     YKRRGWNNNGIPTIEHLTKIGMDFPEVIEVVAPLQ"
     gene            24598..24801
                     /gene="thiS"
                     /locus_tag="PLA17_00990"
     CDS             24598..24801
                     /gene="thiS"
                     /locus_tag="PLA17_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPK84017.1"
                     /translation="MEITLNNEPMTIDGDTITVEKLLELKKYSFRLRIVKINGRIIDR
                     DSYAREIIHDGDTVQVIYLMSGG"
     gene            24798..25253
                     /locus_tag="PLA17_00995"
     CDS             24798..25253
                     /locus_tag="PLA17_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84018.1"
                     /translation="MTAQENNNNGKSRKGDFWQRFTLAALIERMPPWMKNKYILTLLI
                     FIVWIILLDPNNLISRVREIKNRNRLQQEKEYYMSRIEEDRSKLNELRTNNENLEKYA
                     REQYRMKRADEDLFIIVTPEQERKIRRERKVSQTPKPAGRQASEKSKKK"
     gene            complement(25254..25859)
                     /locus_tag="PLA17_01000"
     CDS             complement(25254..25859)
                     /locus_tag="PLA17_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPK84019.1"
                     /translation="MNRISVARDTLILTVMVAAVALLSSGCGKNKPAGAEESVVKEHN
                     GGIAYIEIDSVIANFDMAKDKTAELEEKTKNSEAELASKSQAFDRDVRDFQNKVQKGL
                     VTRATAAEMEQTLQQQQQTLLARRDEMAYNLNEESIVAQRQVLEYINTFLVEFNADRG
                     YQYILARQFPGPVLYSDTTLDITAEVIAGLNAKYSAEKAKK"
     gene            complement(26125..27126)
                     /locus_tag="PLA17_01005"
     CDS             complement(26125..27126)
                     /locus_tag="PLA17_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPK84020.1"
                     /translation="MSLRAQYLAGLLLAFSLIANSQDPQFSQFYANQLYLAPSFAGAT
                     EDYRLAINWRNQWPSIPGVFHTYSISFDKAVPNFNSGIGFLATYDVAGSGDLSTMNLG
                     LLYSYDIQVNDEWHIRPGVNFKFTYLGLDISKLVWNSQITTGGTLPPITPPPFDNVAD
                     IDFAFSGLAYNEKIWGGFNIDHLLRPKTSFWGESTSVPIKVDLFGGFQIISRGRLKKK
                     LEEVLSVAANVMFQADFFQSDIGVYYYKSPLVFGVWYRGIPFATSKEVSAQAGDAIIG
                     MLGIKTDQLNIGLSYDFTISNLITSSGGAYEISLIYSFTSAQVRRGGRIHAIPCPEF"
     gene            27611..>27987
                     /locus_tag="PLA17_01010"
     CDS             27611..>27987
                     /locus_tag="PLA17_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPK84021.1"
                     /translation="MTRILKHPILDIPCNKETSFKYNGTVVKGMEGFTIAAALHQAGY
                     PVHTHSLDNRNRSLECGIGKCGACEMLVDGVVRRICITPVDGVKEVAEIPAGYHSATA
                     VKAKGETRKVYRTTVAIAGAGPAG"
BASE COUNT         7308 a         6783 c         6985 g         6811 t
ORIGIN      
        1 aaagagttta acctgtttct caatgcgctg atgttcttca cgcgtattcc ctgcccccgt
       61 gtcacggaat actctgaagc aatcatgagc aagacgcttc gatactaccc gctcaccggg
      121 tttatcgttg ccgcaattac ggcaggtgtc ctgtggctgg ctttactcgt gatgccggtg
      181 cccgtggcgg tgactattgc cctgactgtt tctgctctgg tgacaggagc tttccacgag
      241 gacgggctgg ccgactttgt tgacgggttt ggcgggggct ggtccaggga caggatactg
      301 gagataatga aagatagccg tatcgggaca ttcggaacca ttgccctcat tctgcttttt
      361 gccctgaaaa tagttgcatt aagcggcctg cccgtggaaa agctcccggc aattataatc
      421 gcggcatatt gcgtaagcag ggtcttcccc tcactgctgg tcaaaacctc tgtatatgcg
      481 cgtaagaatg aaggaaaagc ctttttctca agaaatggaa ttgactggaa atccattgta
      541 atcatatcag ttaccggttt tatccccatg atattgttca attacagatt tgtgattctt
      601 tatactgctc tctctcttgc cttccttgca gctttccgtc actaccttca ccgccggata
      661 ggaggctaca cgggagacac tctgggtgcc gtacaacaga ttacggaact gatcttttat
      721 cttctattaa cagcagtgcc tgcatcatgg atgaattcct ctgtcttgta agacatacac
      781 ggacagccat gtctgaaggt atctgttacg ggcagactga tatcgacgtg gccgacacct
      841 tctgttccga gtatccggca gttgtctctc agctggcagg tattgaattc gaccttattt
      901 acagcagtcc cctgaagagg tgccttcgtc ttgcagagct tttcagcaat gacatactgc
      961 ctgacagccg gctcctggaa gtgaacttcg gagactggga gggagtaaga tgggatgata
     1021 tttttggctc gcctgatggc aaaaaatggt tctctgacta tacttccatg cgctgcccgg
     1081 ggggcgaatc attcatcgac ctgcaacaac gcgcgtggtc attccttcag gatatcagcg
     1141 gcaaggggaa aagaatactt gcagtgacgc atgccggact gatgagggcc atgctggtta
     1201 gcctgggact ggaagaggct gatactgttt ttggtaaaaa aatcccgttc ggcggagtag
     1261 tgatgctcga aaaaactcag caaggatata tctgccggac taaattggaa ccgagatgag
     1321 tattctgttg cgcattccat ggcatttttt ggcatgagca cggcaacata gtttattagt
     1381 actcaatacc ttctcttgct tccactccct cattatagta atgctttatc tctttcatct
     1441 cggtcacaag atcagccata tcaatgagtt cctgcggagc atatctgccg gtaacgatga
     1501 tttcagtctg ctctggtttt gatttaagca gttcaataag catgtcaggg gtgatcaggt
     1561 taaaataaac tgctattgaa gcttcatcca gaacaacgat gtcatagaca cctgacctga
     1621 ctatttcact gatctcctcc atgccggcag cagctgcttc aatatccttc tcacccggtt
     1681 tgccatgaat aaaacaacct cgaccgtatt gtctgagaac tattccgggg aggcaacgtt
     1741 caattatctt tgtttcagag taaggaagac ccttgacgaa ctgtgcaaag aagacctttt
     1801 ttcctgctcc ggccgccctc agtgcaaggc ccagggccgc agtggtcttc cccttcccgt
     1861 taccggtata cacctggatg tacccccttg atggcttcgt tcctctaatt ccgttaaaat
     1921 gcttttcttc cattacatat tttggacata tcagtgtaaa aacaaatatt ctctccttct
     1981 ctgaaaggtt gtgtcacgta ataatctgca gcaagctccc tgttaagttg ctctaatatt
     2041 tcatctgtgt gatacccggg tatcactgtt gtacgtaact gatagctaat gcctgacgat
     2101 ctcagtattt caacagactc agtgatcagt gatgtaatgc cattgacaga acaacctgtg
     2161 atttcctggt actgtgacac ttcaatcact gtcttgatat ccattgccac ataatccagg
     2221 agtttgttca caatcagata tcttaacatt tcagggttgg tgccgttggt atcaagctta
     2281 ataagcaggc ccattgcgtt gaggtgtccg atgaaatcag gcaggtcagc atggatggtt
     2341 ggctcacctc ctgtaaccac cactccatca atccattcct tccgttcctc caggtcacca
     2401 agcacctctt caaccggtat atccctcgtt ttacggataa gatgcggtaa gaccagataa
     2461 gggttatggc agtaaccgca cctgaagtta cagccctttg taaaaatcac cgatgctatt
     2521 ttgccttccc agtcaatcag gctgctgtgc acgattcccc ctatgcgcat ccttccggct
     2581 gttttgatcc gtcatccccg gcggaaacgg caacagggtc actattttca ttcctattat
     2641 acaggctgtt ttttttcgcc acgttatcaa accggaatac cttacggtca atgaactcct
     2701 ttttcttacc atcgttccac tgactgacag gcctgagata accgactatt ctgctgaata
     2761 cctcgcaggc gcttccgcac tcggggcatt caaagtgttc accatggata tagccgtgag
     2821 aggggcatat actgaacgtg ggtgaaagag taaagtaagg cagcctgaat tgcccggcaa
     2881 tctttttaac tatactcctg actgatgctg ccggaatagc cttttctccg gtaaagatat
     2941 gcagaacagt gcctcccgtg taaagagtct gcaactcatc ctggtgattg agtaccctga
     3001 aaacatcatc actgaagttt accgggaggt gtgttgagtt tgtataataa ggctttgagc
     3061 cgttaaggta atctttatgg ttggcagtga ttatatcttc gtactgcatg gtatcgatcc
     3121 tggcaaagcg ataagcggtg ctttctgccg gtgtggcttc cagattgtaa atgtttccgg
     3181 tctcttcctg gaactcaaca agaacatctc tcatgtagtt gagcactcgt attgcgaatg
     3241 ccttgccatc agggtctgca attcccacac ccaggaaatt gaggcagcac tcattcatcc
     3301 cgacaatacc tatggttgaa aagtggttct tccagaaaca gttatacctt tttcggatgt
     3361 cacgcagata gtattttgaa tagggatata atcccttttc agtgtatgat tcaattgttt
     3421 tccgcttaat ctcgagactg cttttagcca gttccatcag gtttctcaac cgttgcatca
     3481 gttgttgttc cgaagaggct tcgtatccaa gtttcggcag gttgattgta accactccca
     3541 cggagcctgt cagcgggtta gaagcaaaaa gcccgcctcc cctcctgttg agctcacgct
     3601 tatcaaggcg aagacggcag cacatgctcc ttacatcatc aggactcaag tcagaattga
     3661 cgaagtttga gaaataggga ataccgtact ttgatgtcat ctcccatatt ggcttataat
     3721 cagggttatc ccagtcgaaa tctttggtaa tattgtaagt gggtatggga aatgagaagg
     3781 gtgttcctgt tgcatctcct tcgctcatca gttcggcgaa tgccttgttg aagagattaa
     3841 tctcatgctg gtagtcgcca taaacagact cgtgcagttc tcccccgtaa acaaccggtt
     3901 catttttaag aaaatcagga accacaaggt caagggtcac gtttgtaaat ggtgtctgga
     3961 agcccaccct tgttgggata ttcatattat acaggaattc ctgcagcgac tgctttacct
     4021 gggaataacc aaggttatca aacctgataa atggagccag cagtgtgtca aagttggaga
     4081 aggcctgcgc acctgcagct tcaccctgca tggtatagaa gaaattgact atctgcccga
     4141 gggcggttct gaaatgcctg gcaggccttg tctgtgtctt accggtaacg cccctgaagc
     4201 cggagacgag caggtcacgc agatcccaac ctacacaata aacactcagg aaacccaggt
     4261 cgtgaatatg gaaataaccc tggcgatgca tcgccgcaat attctcagga tagagtttgt
     4321 tgagccagta ttgcgaggtt atcacagttg agatatgctg attaagtccc tgcagtgagt
     4381 atgatgaatt ggcactctcc tttaccctcc agtctttaag ggaaagataa tcatcaacca
     4441 cctcaatatt tgagaaaatg tctttggtat tacgtaccag ttcccgctgt ttgcgataca
     4501 ggatataagc ctttaccagc cggaaatcac ctgattgaaa aagtattcct tcaacaatat
     4561 cctgtacctg ctcaacggtt ggtatctcca ctttttctgt ttcaagctgc tcaaccactt
     4621 ttgcagacag ttcacctgcc ttctttctgt cggggacgcc tgttgcacgc atggccctga
     4681 agattgcatg ctcaattttt tgtggattaa actcgaccag atcgccgtta cgtttgatga
     4741 tctgccggat agtgtcaggt ctgccattca tcttatttaa agtttaaact gttaataaat
     4801 ggtagcgtgc ggggattctg aaattgagtg gctgccgttg gcagagtatc aatcgccttt
     4861 cacccgaaag ctacgaaact atgcatcggc aggtcttctg gctcgtcctc tttcaaccgc
     4921 cttcccattc aacaaagaaa agtggctgta tgattgaact ttataacagg acttacagct
     4981 acggggatag ctccggattc acaccggatt ccctattaat ctgataagaa ccgatagcaa
     5041 tacaaatcta agtattgtaa aaggagagtc acagattaac ttattaagtt ttgcccggag
     5101 ttttcaacac tcatttctct gtaagcctgt accgactata atcattcgtc aaatcctgaa
     5161 gatagtgtca gtataacagc catgcataat gtgcttcgac tgtaaaatat taaccgcgac
     5221 tttataactt tacggaagaa tagttgtatg acccaaacca gtctgttcac gaacaaaaaa
     5281 tcctaagacc atgaagaata tactgcctgt cctgattcta ttttgcttaa tcacatcatg
     5341 ctctcagaga ccccgcgacg gatacgaatt aattgagcag atgtacgaag cgcacaaatc
     5401 aaactggtat gagactctct gcttctcgca ggaggttctc cattacagga atgacaccgt
     5461 aactgccacc gagatatggc atgaagctta caagtctccc ggcaatctca taatcaagtt
     5521 caatgacccg gacaatggca caggcatatt gtttataaat gacagcatct tcagttacag
     5581 ggatggtgct cttgaatcag tcagatacta tttacacgat cttgttgtgc tgggtcttga
     5641 tattaatact gttttgcccg aaacaactgc cgaacgggca ttgaaatgca actatgatct
     5701 caatcgtctg gcggaaggac aatgcatggg acgcgatgcc tggatagtgg gtgacactct
     5761 cagtaactgc ttctgggttg acaagagttc acatctgttt ctgaagatga gacgccggtc
     5821 aggagagaat tccagggagg tggaatttgc cagatatgat actgtcaatg gttatcccgt
     5881 tgcaacggag atcaggtttt acgacaatga aggtgcactg agcatgattg agaagtattt
     5941 caacatcacc cctgactgcg agttgtcacc ggccatattt agtccgtcag agttcaataa
     6001 tgctaaatgg tgattttttg caatagactt ttcaatctct ccccgggtcc gcgccagaat
     6061 ccaataatga cagatggtga ttacgaaatt attcacatgt aacagctatg tgattgacca
     6121 cccatataac agctatgtaa tggaccacca atgtgccacc aatgtgatga actacccata
     6181 taacagctat gtgagggacc accatcgaag agattaaaac gccatcctgg ataaagctga
     6241 ataaggataa cagataggcc aagcctctct caggcatcct gaccctcggg gaaaccatcc
     6301 tgctttataa ggataataga tatgccaagc ctggagaatc atgctttggg ttgccattgc
     6361 aagaatctgt tattgacctg tgaactcaga gcggagattt cagggatctt ctcaccggat
     6421 ataactgcat tttgtttaaa ttcagccatg gaatcagata acaaaccata taaataatcc
     6481 ggtcatgaaa acaaaaccgt tatcactttt acttgcactt ctcgtcctgg caaccggcag
     6541 cgccctgtca caaacgacag gcaatcctgt caccgacctc ctgctgtcgg catggtcacc
     6601 ccggaacttc accagcgtgc ctgtcactga tcagcagatt gatatgatat tgcagtgcgg
     6661 catcaaggca ccaagcgcaa gaaacgcaca gccatggcac ttcacggtaa ttacgaacga
     6721 agcagtgatg aaggagatca tccccaacat cacaccgggg aatctgatca tagtcatctc
     6781 cggcaaagtg tcacaatcag gcgccacacc cgatttcgac tgcggactgg caacacagaa
     6841 catgttcgtc gcggcaacat caatgggcct cggagcaagg atttacggcg ccccggtagc
     6901 cgctgtcaga agcaaaaaag aggcactgca gatacccgag gaattctctc ctgtcatgat
     6961 gattcgcgtg ggtaacatgg agaaaagcag tgacgccatc tcgggcgcat cgccccgcaa
     7021 aacgaaggag gagaccgtta actgggtcag atagcttcgc aacctgtatt aagacaacaa
     7081 catcaccaca ggatttgcag caaagttttt tcagaatcat aacattttat cgaataagtg
     7141 taataaatac atttttttat atatttatag ccggttaata gttcgcccgt cgggcggaat
     7201 gaccacaatt ctcttatatc cgtcaggaga atttgttaaa cttgctgata cagtggcgga
     7261 taaacattaa gaaaacaatt ctgatcatga agattaacag gctgttcatt gttattgtat
     7321 tgatcttatg ctcgttggtt acatcccctg gatttgccca ggatggtgac cagatgcttg
     7381 acggaatagg tgaaaccgga ttgatatcac gctaccagtt caagggaaat gacagggact
     7441 ggtcgcgcaa caacctccat gccatcaccc gggggacgga cttcagattc atacctgaca
     7501 gcctcttcgg taatgttctt gccctttcgg gcgaaagcga gacatacgtt tccatcccgg
     7561 gcgaggcggt gtcaggcgaa gaatcgctta ccatctgtgg ctggataaac cttctctctg
     7621 cacaaccggg acagatgttt tttgatttcg ggaagaacag caactcgcac ttctttgctg
     7681 ccccggcagg aacgaaggag aagcccggac tacaggcagc atttgtggcc ggatcaaaga
     7741 tatataccac ttcctctgat gcagtacctc tcaagaaatg gagccatctg gcagtagttc
     7801 ttgatatccc ggcgaagacc ctgagtacat acctgaatgg tgcgctggtc gccgaggtta
     7861 aggatgccac tcttgacctg tcacagctgt ttgacactga ttcagaaaaa aacaaccagc
     7921 tctatgttgg caaatcaatc gacggtgaaa agagtttcct gaatgcaaag ctctccgatt
     7981 tccgcattta ccgcattcca ctgacatcca gacagatcgg gagaatctat ttcaccgctc
     8041 tgagaggcgg cagccagcag gcagcacaca ggggtcagcc tgaacagacc ctccaggttt
     8101 ttcctgcaac aacaccgcag ctgtacaatg cgtttctggt cagcattcct gacacgcagg
     8161 tggagaccac tgttggaaac ctcccaagac tgccacgttt tgtaaaaggg gtttacagca
     8221 atggtgcgga gggctcagat gtgagagtga tctggcctgc tcccgaggat aacagccagg
     8281 tacttgtccc cggaacatac acggtgaccg gaaaggttgc cggtacaagt ctgcagccca
     8341 gggtggtagt gaccgtcaga tcagcggaag cccctgccac accgaatcgc acgctggagg
     8401 ccttcaggct tgaccaggta aggcttgatg cagacattca caatcaggag acaaagttca
     8461 tcgagaaccg tgacaagttc ataaaaggcc tcgaggccac caatcctgat aatttccttt
     8521 acatgttccg caatgccttc gggcaggagc agcccgtggg cgcgcagccg ctcggcgggt
     8581 gggacagcca gcagaccaag cttcgcggtc acgctaccgg acattacctc agcgccatcg
     8641 ctcaggccta tgcaagcacc ggttataatg cggagctccg ggcaaagttc gcaggcaaaa
     8701 tggattatat ggtgaaaacg ctctatgagc tctcgcagtt gtcaggccag ccccggacag
     8761 ccggcggaga gtatgtggct gatcctacag ctgttccgcc cgcggcggga aaaacaggct
     8821 acgactctga cctgagcgag gcagggatac gcaccgacta ccagaactgg gggaaaggtt
     8881 ttatcagcgc ctatccgcct gaccagttca tcatgctgga gaagggagcc acctatggcg
     8941 gctcgaacgc tcagatatgg gcaccatact acaccctgca caagatcatg gcagggctga
     9001 tggatgttta cgaggtgagc ggcaacggaa aggcgctgga gatagcgaag gggatggcca
     9061 catgggtata tgcccgcctg agccggctgc caaaggagac gctgataagc atgtggaaca
     9121 ggtacatcgc cggggagttc ggtgggatga acgaggcact ggcacggctg tcacgtatca
     9181 ccggtgatga cagcttcatg aaagctgcac agttgtttga caacatcagg ctgttctacg
     9241 gcgatgctga tcatgcgcac gggctggcaa agaatgtgga tatgttgcgc gggctgcatg
     9301 ccaaccagca tattccgcag atcgtcggag caatagagat ttaccggggc accggggcac
     9361 cggagtatta taacatcgct gacaacttct gggacatggt cacacacgac tacatgtaca
     9421 gcatcggtgg tgttgcaggc gcacgcaatc cgaacaatgc ggagtgcttt accgcgcagc
     9481 cggcatcatt gtatgagaac ggtttcgccg caggcggaca gaacgagacc tgcgccacct
     9541 acaacatgct caagctgacc gggagcctct tcctcttcaa ccaggatgcg gcgctgatgg
     9601 attactatga gcgcgggctg tacaatcata tacttgcctc ggtggccgag cacaacgccg
     9661 ggaacaccta tcatgtgcct ttgagacccg gctcgatgaa gcagttcggc aacccgaaca
     9721 tgaacgggtt ctcgtgctgc aacggaacgg ccctggagag cagcaccaag ctgcagaact
     9781 caatttactt caggagcgcc gataacagtg cgctgtatgt gaacctctat gtgccgtcaa
     9841 cgctgacatg gactgaaagg aatattacta ttaagcaggc cacggcttac ccgaaggagg
     9901 accatacggt gctgacaatt gacggcaagg gcaggttcga cctgaatgtg agggtgcctc
     9961 actgggcaac gaagggattc ttcgtaagga tcaacggcaa ggatgtgaag agtgaagcgg
    10021 ttccggggag ttatctgacc ctgagtcgca agtggaagaa gggcgatact gttgagcttc
    10081 gcatgccgtt cagattctac cttgaaccgg tgatggatca gcagaacatt gcgagcctct
    10141 tctggggtcc ggtactgctg gcagcgcagg agcccgctcc ccgcaccgag tggcgcaagg
    10201 tgacccttga tgcttcagat atcagcaaat caatcagcgg tgacccctcg aagctggagt
    10261 ttatgattga tggtgtttta tacaaaccat tctttgacac ctacgggaga cattcggtgt
    10321 accttgatgt ggaattgaag tagggattcc tgcgttcgcg gccggcgaaa ggtatgcgaa
    10381 gcactctgat atagtgttga ggtagaaatt ccggagttgc atcaggaaat aggcattcag
    10441 tctatcttga tatgtagcct tccgcgtccg gcgaaaggca ttcagcctgt cgtgatatgg
    10501 agattcccgc gtgcagtaga agacatactg tgtacccgga aaccagtcaa ccggttatct
    10561 catgatgccc cacggataga gctttctggt ggcacagaat gatccggcgg gtgtctcggc
    10621 aagatttaca tgtgcggtta gtctctcctg cttgccgcaa tatggttcaa gtatctgtat
    10681 gatgtattcg cgactttcca gacccctgac cgtcagattc acatcgaaca ggcagttgca
    10741 gctgcaggcg gcactctgtt cgttctcctt gataaacaat gtatcaccgg ctaactctgc
    10801 atgacaggtc agcttgccgg gacaacagtt aaagccagcg ttgatatgtg ttatctgaag
    10861 tgtcccggaa gaggcctcga aaacatagtt aatgcatgag agggtatcag gggtagtgcc
    10921 gtcaaccgac cctgacttga ggctcttgca ccccgtgtga tcaataacct cggcggtgat
    10981 acgctcacca tacctctcac agcctgacag cagtatcgct gctgacatca ggactgccaa
    11041 cactgaaggt aacaccttgt ccgatatctt tttcatgata aattttctga ttttcaagtt
    11101 attcattatt acaactaatc tgccgcaagt ctcgtgccaa aactgatccg cgcaggaaca
    11161 aatgcttatc cggaatgcta tttcgtaaca aaaggtgccc tggcctttca ttagttcaag
    11221 ggcaatgcaa tttaaaagag cgtttcatct gagacagatg cacccgcttc atatgcaaag
    11281 gtaaggcgct atgtttgatg aaaaagattt tttcccggca acaactatcc ataactgaaa
    11341 atttccttaa tattgaaggc tgatcccggc acttattgca acagtgatgc ggacacctcc
    11401 aaatgattga tttacatcac acagggtttg acaacgagaa atacctcagg gagcagacag
    11461 cagctatact tgagcgtgtg aaccgtttca acaataagct ttacatcgag ttcgggggca
    11521 aagtgctctt tgacctccat gctgcccggg tattgcccgg attcgatccg aacatcaaga
    11581 tgaggctgct gcagatgttg aaggataaga tagatgtgat tctatgtatt catgcgggtg
    11641 acatcgagag aaagaaggta cgggcagatt tcgggataac ctatgatgtt gatgcactga
    11701 agacaattga tgacttcagg gagtgggaca ttgatgtaac ggctgttgtc atcacccgtt
    11761 acacggggca gtatcccgca agagttttca gaaacaagct cgagcacagg ggagtgaagg
    11821 tatataccca ttcacccaca aaggggtatc ccacagatgt ggaactgatt gtaagtgatg
    11881 aggggtatgg tgccaatcaa tatataaaga ctaccaatcc gattgttgtt gttaccggac
    11941 cggggccggg aagcggtaag ctggccacct gtctgtgcaa tctataccat gagtaccgcc
    12001 gtggtgtcaa ggccgggtat gccaagttcg agacctttcc catctgggat ctgccccttg
    12061 accacaaggt gaacatagcg tatgaggctg ctacccttga cctgaaggac gaggtactga
    12121 tagacagcta ccatctgaaa cgctatggca caaacgtggt caactataac agggatattg
    12181 aggctttcca tctgctcaaa agaatatttg aaaagatcac cggaggcgag tcaatgtacc
    12241 agtcgcccac cgacatgggt gtcaatagag ccagcgccgg gattgtcaat gacgatgtga
    12301 tacagaaggc agcacaccag gagatcatac gccgttactt caggtgtgcc gtggagtata
    12361 ctataggact ggccgacaag gagatactgg agcatgctgc gaagattatg gaaaaggtgg
    12421 gggcgaaacc ggaggacagg aaggtagtat tgccagcaag gaaagcgatg aaggatgcgg
    12481 agaagggtgg caagggtaat gccggcattt tctgcggcgc ctccattgag ctggcggatg
    12541 gtaccattat taccggcaaa aactcgccgc tgatgcatgc cgcatcaagt ctgattctca
    12601 atgccacaaa atatctggcc gggctgcccg acagcctgtt gctgctgcca aagaacctta
    12661 ttgactctgt aacttacatg aagaaggaca tcctcaacgg caaggaggtt agcctcgatg
    12721 ttgaggaaac acttatattg ctgggtataa gtgcgctgac caatcctgcc gcccaggcag
    12781 cactggaaca actaaccctc ctccggggtt gcgaggtgca tcttacccat ataccaacct
    12841 ctggcgatga ggccggactg cggaagctga atgtgaacgt gacgtgtgat ccggaatact
    12901 ccagcaagag cctgtttgtc agctaatcag gcaacaggca gcagagggac gggcgtgaat
    12961 aaatgacccg tcacaattct ccccgtaaga attaaagatt gcagatcaga tattttctat
    13021 ctcctcggta ccccaaccca atagaggtca aaccctcctt tcccgcctgg acggttggag
    13081 gaaaacacca taaggtcatt gatgaacgac tctccacccg gaaccagtat cggccggtac
    13141 tcgtcatatt ccgtgtttat caggttaccc atattgaccg gctccgtcca cgtctggcca
    13201 ttataaaccg agtaccagag atcaaatccg ccaaatcccc cctcacggtc agaggtaaag
    13261 accatcacgt ttccggcaat gtaaggacac ttgtcatcag cgctgctgct cagaaccgat
    13321 gaccgggtga tggtgacggc agccgactca ctgatcagct tcccctcttc gccggtggcg
    13381 ctgaaaatat caaaagagcc gtcgcggtca gacatgaagt aggctgtctc actgctctga
    13441 gcggctccgt gatggaggga gaaatagcct tcgttgaatt ccgtattcag ttgtgtcacc
    13501 tcgaacggat cacctgacgg atcaaaacca tcattggcaa attcatatct gcagcagaat
    13561 atatcaaggt tcccttccgg gtcactgctg taaaagaacc gtctctcctc ctcacctttc
    13621 ttataataat ggaagtatgg gtagtcactg ataaaatatg gtcccagctc gttgcagtca
    13681 ctattgacag cttcaagaaa tcctgattcc caagcttttg cctgcatttc gaatccgcca
    13741 tctatcaggt caaatatgat atttccgtgg tatccgataa agtcgaaatt atcgccgttt
    13801 gaatttctgt tgcttgaaaa gatcaggctg aaagtcatat cggcccatga tatcttcagg
    13861 tcggaattgt aatcatcgcg actggagttc atcgttgaaa agttcgccgg aaccggcgga
    13921 atgatacctt tgtcgaaatt aagcctggga ctgttgcagg aactgaaaag gagtggtatt
    13981 attagaagga atgctgatct tttcatagcg aggaaatttt aaggttcatg atgaaaccga
    14041 catttacact ggcatgattc atcaacatat cgaagtaacc atttggccct gccgggtcac
    14101 tgaatgaggg ctcagtagtt gaaacactgc ctttaattga cactccgtag cgtttagtca
    14161 gccagtagtt gatcttcagg ccggcatctg ccacaaggtt tccattattg gttttggcaa
    14221 tgctgttgat cactccatac tctgagttcg ttacccgcag atcagggtga atgcatgctg
    14281 catatccgag gtgaaagagc agcatgacct cggtatgttt tatctcaggg attttaagtg
    14341 tgaatcccgc caggaactta atcatctggt agtttccggc tgatacatcg ttgatgccgt
    14401 aacctccgag ggtacggtca tattcagact ggtatgcctt tccattgaaa aagaagctgg
    14461 catagccaat tgatgatgag aatccgaata ttttgccgta atagaggtat tccgccccga
    14521 ggttcggacc aggggcggcg aaacctgcat cataggtgaa tgttttgagc gcaaactcac
    14581 caagcggtat cgaaacgccg gcgttggcac caaagactga ttttctgatt ttttgctgtg
    14641 cctggcatgt tgcagccata aggaccagca gcgccaggat ggcgaagact tttctcatta
    14701 cattcaggtt aggccgggaa cagatacccg gtacaatggc aggcaacaaa tatcacgcca
    14761 aaagagtgct tttcccccct cccatccgga ctattgtctg ataagtgaat tatgacgatg
    14821 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgacgatg tcgatgtgac
    14941 attagcggca aggcatacat ccggaccatt gtctgataaa tgttctgcag cactactgag
    15001 gtgattctgc ctggaatttc tcaatctgta aagaataaag tggaattgga ttgggaaaaa
    15061 ctccggtttt ttttcactat cttcacccct tctaattagt gtttcatata tggctgatat
    15121 tcagacaatt tatcaggaga ctataaaatt tgcggcagaa aagcacggag cgcagaaagt
    15181 aaaaggaaca agaattccgt atgttgttca tctgagcaac gttacaatgg agatattcat
    15241 ggctgcacgc caaacacgcg gatttgacct cggatatgct ctccaggtgg ccctcctgca
    15301 tgacacgata gaagacacag gaacaacacg ccaggaactg gagatggttt tcggaaagga
    15361 tattgccgat gctgtctggg cgatcaccgg agatccgaag ctgatcaagg agtacaggct
    15421 ctctgacagc ctggccaaga taaggcagtg tccgcgagag gtgggagcag taaagcttgc
    15481 cgaccgtatc acaaacctgc aggctccgcc gaaaagctgg agcaaaagcc gtatcagtga
    15541 ctacctgttt gatgccaagc atatactcat ggctctgcag ggatcgaatg agtatcttga
    15601 aacaaggctg gcagccaaga tagaagagta cggtaagtac ctgaatgtcg aaaattctta
    15661 aggtctgaag gtagaaaagt cccggagtcc tgaggtcgag tagtcttaca gtctgaaggt
    15721 ctgaagacag aaaagcctta gggtctgacg gcagaaaagt cccggaatct gaagtcgatg
    15781 aacaagtttg catatctgtt ttcactttgt ctttttctca gcaccctgtt tctcagcggg
    15841 caggcgggca gtttcagctt cgccctgatc actgacaccc atatagggag cggcagcgct
    15901 gatgaagacc tgcggcggac agtagaagat atcaatgata ttgactccgt tgtctttgta
    15961 atagtggcag gtgatgtgac ggagttcgga tcggacgctg aactgcttct cgcaaaaaaa
    16021 atccttgaca ggcttgagaa gccctggtac attatccccg gtaaccatga caccaaatgg
    16081 tctgagagcg gtgccaacag tttccgcatc gtctttggtg acgaggcatt tgtcttcaac
    16141 cacggcggat ggctctttat aggcaccaac tccggcccga acatgaggat gggcccgggt
    16201 cagataccaa gagaaaatat tgtatggctc gattctgttc tcagcatccc ggccaacagg
    16261 gcaatgaaga tcatatcagt caatcactat cctcttgaca atgggctgaa caactggtac
    16321 gagctgaccg acaggctgaa gcagcttgat acgaggctgg ccctgtgcgg tcatggtcat
    16381 tcaaacagcg ttctgaattt cgaaggtatt cctgctctca tgggtcgttc caacttgcgc
    16441 gcgaaccatc ctcagggagg atacaacatc atgaccatag tgggcgacac tctgcttacg
    16501 gcttcggtca ggcacccggg aacaggtgaa gaaacggaag cggcaaccgg ccacagtgca
    16561 gtcacgatgc ctgtctgggc ctcggtccgg cttgagaggc atgatttcag cgctgacacc
    16621 accagatggc cgagacctga ctattcaatg aatgaacggt acagccaggt gcgcgaagta
    16681 tggcgtttac aggaggaagc cgacatcggt gccggaaccc tggttacagg gaagctggca
    16741 ctcatcacca ccaccgccgg tgaagtgaag gcaatctcac tcagaaacgg gcgtgagaaa
    16801 tggtccttcc ccaccggggc aaagatttac ggcactccgg tttctgacgg aaagagagtt
    16861 attgcggtat cagccgacgg catggcaagg tcccttaccc ttgagagagg caaactgttg
    16921 tggagttatg acacgaagca gccggtggtg gcctcccctg taatcagcgg ggagcgcctc
    16981 ttcattgcgg gctcgtcggg ccactgtcat gcacttgaca ttacaagcgg ctcagtgctc
    17041 tggaccaacg gtgatattga cggtttcgtt gaaaccatac ccctgatata caaggaaatg
    17101 ttgatcttcg gcacatggaa caaccggctt tatgctcttg acattgcaac aggaaccata
    17161 aggtgggtat ggaacaccgg gtattcaaac aggatgctct cacccgcagc atgcgtacct
    17221 gtggctgtcg gcgaccgcct gttcgtggtg gcgccagaca gaaagatggc ctgtcttgac
    17281 gtcaataccg gggaactcct gtggcattcc gacctcggcg gggcaaccgt cagggaatcg
    17341 atggggctct cggaggacag aagcctggtg ctggccaaaa caatggacgg cagggtcatc
    17401 ggtgtcagtg cttcgggcaa cagtccggag ataatatgga agtctgattt caacatagga
    17461 tatgacattg caccgggggt gattacagag cgtgacggaa tcatattcat tccctctgat
    17521 aaaggtattg tgcatgctgc ctcgcgcgaa aacgggaagc ttctctggag ccacaggatc
    17581 tcatcatgcc ttatcaacaa tatcgtgccg ctcaaaggta ataacctgat tgtcaattct
    17641 atggatggga tagtggtcag gctcggctgg taaggccgga aggctcaggc cctcgttaca
    17701 ggtatcctgc tctgcgtggt tcggcttcgt ccgcaggtac ggttcaacct cgtccgcagg
    17761 ttcggttcaa cctcgtccgc aggttcggct caacctcgtc cgcagattcg actcaacctc
    17821 gtccgcagat tcgactcaac ctcgtccgca ggtacggttc gccgcatggc ttatcaatct
    17881 gaaggtccgg tttgccggga gtttgagcag gagaaccaat ggcaggtgtt ttgcaaaaag
    17941 attttgattt tagtcgccaa aggtttatct ttagtgacta actgatcaga ttttctgaac
    18001 ataattatat aatacctcta aaacaataac gaaaattatg gcaggattta ttgatgaatt
    18061 ccttgagagc tatggccctg aagtgacaaa acagatgtca agcaacttca atgttgatca
    18121 ggccacggtt cagaagctta tccctcagct ggcgccactg atccttgcgg gtctcaagaa
    18181 gcagaaagac acccgtggcg gtgatgagag ggttgatcac atcctcaaca agtatggtga
    18241 ctcctccgtg ctcaacaata tcaaagatct ggtctccagc aaggcccggg caaaggatgt
    18301 tgatccgaac cttggcgggc ttctgggtga tgcaggaggt gtgcaggctg cccaggccct
    18361 ggcaaagaag atgaacattg atccttcgac gatcatgaag atgatcccgg cactggcacc
    18421 cctgctcctt ggagcactta ccaaaaaacg tgacaccggc ggcaaaggta tttccggtgt
    18481 aggtgcactc ctggatgctg acggcgatgg cagtatcctt gacgacgtgg caggattcct
    18541 gctcaagagt ggcagctcaa cccggtcggg cagtggcggc ctcctcggtt caatcatcgg
    18601 agggatcacc cgccgaaagt agtccttgtt actatgaaaa aaccggtact tttcctttct
    18661 gttctcctgg tactctggat tgccggcgca tcatactggt atgtatgcaa gataaggtgc
    18721 gactgcaaga aggcagaagc atcttcagtt gaaatgcagg ctgccccgga taccctggcc
    18781 ccgtcacctg aacagctttt actcgcctct gtcaatgagg caaaacagta tctctctgaa
    18841 tcaggagtcg ggaaaggatt cttccccacc tcatccgccg ccggagacat gagcggcata
    18901 agtgatgaat accttgagaa gctgaaactc gtgcttgaca atgacccggc tgcccgtgtg
    18961 gaggtgaccg gccatactga catcacgggc acgacttcct tcaataaaga gctcggcctc
    19021 aaaagggctg aattcgtaaa atcatacctg gttaacgcag ggataaaagc ggaacagata
    19081 atgacatcat cgaagggtcc gtcagaaccc gtcgcgtcaa acagcacgcc cgaagggaga
    19141 gccgctaaca gaagaagtga aatcaaagtt ataatttaaa catcatggcc gaacgtttta
    19201 cagcagatct gctgttcatt attattgtgc tgctggtggc agcattgctg ggtttcctca
    19261 tcggttacta ccttgaaagg ggccgatgca ggaaacggat agccattctg gatgaggaga
    19321 tagaagggct gaaagcgaag atacgcagtc tggaggagga gaagagagcg cttgaggaga
    19381 agctgaggca ggcagaggag gaaaaaaaca accttaccgc aaagattaac ggtctggagg
    19441 aagagaaaac aaaactgcgg gatgataccc gcagaatgga tgacgagatc accagcctca
    19501 aactgacaat agacagactt gagaaggaag ccagggagct caggggagat cactccgaag
    19561 gcagtattgc gacggatacg cccctggccg ccatggctca tgaagaattt aagcctgacg
    19621 accttgaggt agtgataggt atcgggccga aaatcgcatc gttactcatc aaccgcggga
    19681 taacaacctg gaaggcactg gcagaaacat ctgtcgctta cattcaggag atactcgaca
    19741 aagacggcgg tgaagattac cgtatccaca atcctgagag ctggccacag caggcccgcc
    19801 tccttgatga gggccggtgg gatgagttcc gggaactgcg ggaacaactc aaatagaaaa
    19861 atctgcctgt caggcaggta gcgatgggcc tcagacccat cgctacatta caaatatgca
    19921 catgttctgc atcagggggg cagggtgcct cacaaccgta taatctgcct ccctggcagg
    19981 atgcatcaca accagacaat ccgcatctgg ggcagggtgt gcatcgccaa cagataattt
    20041 gcatccgggg caggtggcga cgggtctcag acccgtcgtt acgtagaatt atgcacaatt
    20101 tttctcccga aaaaggagaa aaaacctata tttgtttatc agggtgtgga ataccattga
    20161 taaatacata ataaaaggag gtgctgatga ctttcaacga gagtttcttt taccgtcccc
    20221 ggaagtacaa tacaattgaa ttatggaaag atgtaacgcc tgaaaagtgg aatgacgccg
    20281 gatggcagag aaagaattct atcaggtctg ttgaccagtt gaaaaaggtg gtcaggctga
    20341 ctgatcacca ggaatcagaa ataagaagaa ctatagtagc gctgcggcag gaaggcaagg
    20401 aatcgctcag gatcactccc tactatgctt cgctgatgca ggaagatcca ttcaaccctc
    20461 tctttttcgg agagaggtcg cagaaaaggc ttgatcccat attctggcag agcgtcccta
    20521 ctcctgccaa cctgatgttc cccgatgctg gtgtcgaggg agccatgagc gaaggatcac
    20581 gcagttacgg tgcagcttat cagcgctacc caaacagggt ggccctcttt gttgccgaga
    20641 acaccagttg cgcatcctac tgtgttcact gtcaacgggc aaaaacactc gacaccactg
    20701 ccgacgtgaa caacagggaa gttgacaggg gactgtttta tataggttac aacaggaaca
    20761 tcaatgaggt gcttgtcacc ggaggcgacg cactgatgac cagcttcaaa cggctggagt
    20821 acattcttgg tgaactgagc cgcattcccc atctgcggat cataaggata gccaccaggg
    20881 tgccggtggt gctgccgatg ctcattactg acgagcttct ggagatgatc cggactgcgt
    20941 caaacagatt caacagggga ccggaaaagt atgtctactt catgacccac atcaaccatt
    21001 accaggagat aacggctgat ctggccgccg ccgtgaagaa gatcaccagc catggcttta
    21061 caatccgcaa tcagacggtg cttcttaatc atgtcaatga tcactaccgg accctggcag
    21121 aaacattcag gcggatgttc tggataggag tccaccctta ctacctcctg cagtgccaca
    21181 aggaaaaggg gatagtccac ttcatcaccc ccattcaggt gggcaagatt tacataaagc
    21241 acctgcaggg atggataagc ggtatcacgg tgccgcgcta cgcagtaaat atcgagggag
    21301 gaggaggcaa agtgctgttg atgccatcag ggcatgacac catgaacctg gacacgagta
    21361 ttgatgacaa gatctccgag agctttgcca atgtatatac ctgggatcac aagaagatag
    21421 tgaaatacga agccctgggc agggccacaa gggaggagta taagatggct gtcggaataa
    21481 tggacaggtt cattggccgc aagggggtgt tcgtaccgcg ggttataata gtggatgaga
    21541 aggggaatca cctcgagaca accaaccggt tcaggctgcc tacctttgag aaaacgaaga
    21601 aggcagccct gctcaattat gagttgtaca acggagatat gcccctgacc aatccggtgg
    21661 atacatattc tgaaatagag gctgctttcc tgcggtcagg ctacgcatca taatgtgata
    21721 atctgcatcc cgggcagggt gcacatcaca accagacaat ccgcatccgg ggcagggtgt
    21781 gcatcacaac cagacaatcc gcatccgggg cagggtgtgc atcgccaaca gataatttgc
    21841 atccggggca cgtggcgacg ggtctcagac ccaccgctac attacaatat gcacatgttc
    21901 tgcatcaggg ggaggtgcag ggcgcatcac aaccagataa tctgcccccc gggcaggtgg
    21961 cgacgggtct cagacccgtc gttacatcat catattaaaa ttctcctgat aacaggtgag
    22021 accctgttat tgcaacgggg caaccacctc tatcacctcc ggaaaatcca ttccgatttt
    22081 tgtcaggtgc tcaattgtcg ggatgccgtt gttgttccat ccgcggcgct tgtataccgc
    22141 atcgcgaagg aggtcatact gcttctcgcg gtactccctt gtcaccttca tcttttcttc
    22201 aatgctcatg ccctcggggt caacaccaat cttctcttta agctgcttgt cataccgctc
    22261 ctgacgggag agatactcgt caaccgtgac gggtccgcat gcgcgataag gctgatcatc
    22321 atccttgcgt ctcccgtaac cgcgacgtat gttaaacact ctctggaagt tatatactct
    22381 ttccgagtcc tctatcagcc tgtgcttatc gaactccctg cctgtcaccg ccttgtagat
    22441 ggtaacgtag ttgtctacgt gctcaggcac ctttgccggt tcatcagtct gggcattgtt
    22501 ttccggttcc acatcgttcc atggcagttt gcaaaggcct accaggccga accatgtacg
    22561 gaacatcggg aagtagtgaa gcgcttcggc cttatcctcg aatgtgggga tctggttgtt
    22621 gaccatatcc atgaagatga gccatgcctc gtcatgctgc ggacccttgt tggtaagggc
    22681 ataaccaccc tgctgggcaa gagactcttt agacatatac tgtgagtatt cgagcccttt
    22741 gacttccatg cctatatcct gcatgaactg cgggtcgccc catcccttct cggcaaaaat
    22801 ctttttcatc cggcggatac cctgtccggc tatcaggccg aagccctcgc cccgtgacac
    22861 cctgtgaaga agctccagtg catcaggaat gttcccgaat ttcagttcga gaccgccggt
    22921 gcgctccttg ttcaggatgc cgttctcata acactcctgc atgaatgcca ggctggtgcc
    22981 ccatgtgatg gtgcagatgc cataggtgtc acaatagaag ttggcctcga tgatatatct
    23041 cggatcaaag aaccctccgt tggaacccag gccggcagct gtttcatatt ccgggccgtc
    23101 aacaatcacc ttctgaccgg catatggtcc ggtagttatc tcgtagtcgt caacagcctt
    23161 cgcgcatgcc atgttgcatc cgatccagca accgtcgggg atgttttgtg tgaaaaacga
    23221 tttccagacg gcggaatcaa ttctgggcgc gtctttgtgc gatccatatt tgtaattatg
    23281 agtaggcaga agatcatggt catccattat ctccataagg tgggctgtac caacctgacg
    23341 cattctgcac tggctatcgt caagctcgcg catctcacgg ttaaagcgac gcccgcgctc
    23401 ctgaatggct tcgagatcaa ccacgttatt gagattgccc tttacgccgg gaattttgca
    23461 gacaactgcc ttgatctttt tgttgcggaa aacggagcct atgccgccgc ggccggcctg
    23521 cttcatcctg atcttcttgc gcctgccgtc atagaaggtg aagttcagca tgccgatgag
    23581 cgagttgtca gctgcagagc cggtggaaac aatacctatg ttcttcctgt cagactcatc
    23641 attggcgaac atttccgtga gctgctcggc aagcacatga ctgtcaatgg cttcggccgg
    23701 cgcctcgaaa acctcaacat aatggtccac gccgttgata aaaacaatga catcattggc
    23761 ggccttgccc tgaagttcga tggcatcaaa acctgaaaat ttcagaaaag gtccgaaaaa
    23821 gcctccaaca ttactgtcca tcaccgaatc agtctgcggt gagatggtta ccaccagcga
    23881 tttgccggca cctgagtatt gggttatacc acctactggg cctgacgcta tgacaatttc
    23941 attatcgggg tcatcccacc tggtatccgg tcttgtggca tcccagagga gtctgagacc
    24001 atatcccttg ccaccgatga atttttcctt catcaccagt ggaacaggtt tatcctttat
    24061 ctcgcctgtg ccaacgttga tgtacagggt tttatccgta tagcctttgt caatgggagt
    24121 ccatttgtag tgccacctgt taatcagttt gtgttttgcc ttgagttcac tgatgtccat
    24181 aattatatct ttttgttttt tcgatggaga tacttacaaa tttcgtgtat aaatatccgt
    24241 aatctaatga taatagtcaa tatgcagccg attgccggac ggaatgaagg agctgccggc
    24301 tacaatcaaa aaagagtcaa cgccattcag gcattgtcaa aattgccaat tgcccattca
    24361 gcttcgctgc attttctgtc gtcgcccggt atagttttgc aaagcaacac ttttgctcgt
    24421 cgtggcaaaa tccgagcaag ctagtcttct gctcaccgac ttaccgcaaa atttcatgca
    24481 agcctgactc tgcactcact cctaagaaaa ttgctaattg cccattgtta attgctaatt
    24541 gcccattgtt aattgattta atttgccatt cccgatcagc atgataactt tgcacacatg
    24601 gagattaccc ttaacaatga gccgatgact attgacggcg acaccattac cgtagagaaa
    24661 ctacttgagc tcaagaagta ttcattccgg ctcaggatag tgaagatcaa cgggcggatt
    24721 attgaccgcg acagctatgc ccgggagatt atccatgatg gtgatacggt gcaggtaatc
    24781 tatcttatga gcggaggatg acggcacagg agaataacaa taacggcaaa agccggaaag
    24841 gcgatttctg gcagcggttc actctggcag ccctgatcga aaggatgccg ccatggatga
    24901 agaataagta tatccttacc ctgctcatct tcattgtctg gatcatcctg cttgacccca
    24961 acaacctgat ttcgcgggta cgcgagataa aaaaccgcaa cagacttcag caggagaagg
    25021 agtactacat gagccggatc gaggaagacc gcagtaagct caatgaactg cggaccaaca
    25081 acgagaacct tgagaaatac gcgcgtgaac aataccggat gaaaagagct gacgaggatc
    25141 ttttcattat cgtcacgccc gagcaggaaa gaaagatcag gcgtgaacgg aaggtgtcac
    25201 aaacaccgaa gcccgctggc aggcaggctt cggaaaaatc aaagaaaaag taattatttc
    25261 ttcgcctttt cggcgctgta tttggcattc agaccggcga tgacttcagc cgtgatatcc
    25321 agtgtggtgt cgctgtaaag caccggaccg gggaactgcc tggccaggat atactgatat
    25381 ccgcggtcag cattgaactc aacaaggaag gtgttgatat actcaagcac ctgcctctgt
    25441 gcgacaatcg attcctcatt gaggttgtaa gccatctcgt cacgtcttgc cagcagtgtc
    25501 tgctgctgct gttgcagtgt ctgctccatc tcggcagcag tggccctggt gaccaacccc
    25561 ttctgcacct tgttctggaa gtctctcacg tcacggtcaa aagcctgtga cttggaggca
    25621 agttcagcct cggaattctt cgttttctcc tccagttcag ctgttttgtc ctttgccatg
    25681 tcaaagttgg caatcactga atcaatttca atatatgcta tcccgccatt gtgttcttta
    25741 acaacagact cttcagcacc ggctggtttg ttcttgccgc atccggaaga taaaagcgcc
    25801 accgcagcca ccatcactgt taatataagg gtgtcccttg caacagatat tctattcatt
    25861 tcataaattg ggttaaggtt caacaaattg ttgcacaaac atacataaat tttaaatacg
    25921 acaaggatag ccggaaagtg ttgttgcagg tgaggtgaaa tccggattca gaaaagagtg
    25981 gtaaaccagt tacagttggt gtaccatggc cgcatcagaa aagaggggga aaatatgggt
    26041 gataaccagt gacagttgcc gtgctgagac cggaatcaga aaagagagga aaaatcagta
    26101 acagttggcg tacggagacc acaatcagaa ttccgggcag gggatggcat ggattcttcc
    26161 gccacgtctt acctgcgctg aagtgaacga atagatcagc gagatttcat atgcgccgcc
    26221 ggaggaggtg atgaggtttg aaattgtaaa gtcatagctc agccctatgt tcagctgatc
    26281 ggtcttgatg ccaagcatac cgatgattgc atcgcccgcc tgggcagaca cttccttcga
    26341 ggtggcaaag ggaatacccc tgtaccatac tccaaacacc aggggactct tgtaatagta
    26401 aacaccgatg tctgactgga agaaatcggc ctggaacatc acattggctg caacagacaa
    26461 aacttcctca agttttttct tcagtcgccc acggcttatg atctggaatc cgccaaagag
    26521 atcgactttt atcgggaccg aggtgctctc accccagaaa gatgtttttg gtctgagcag
    26581 gtggtcaata ttgaatccgc cccatatttt ctcgttatag gcaaggcctg agaaggcaaa
    26641 gtcgatgtcg gcaacattgt caaatggagg cggcgtgatg gggggcagtg ttccgccggt
    26701 ggttatctgg ctgttccaca ccagtttgga gatgtcaagg ccaaggtagg tgaacttgaa
    26761 attcactccg ggccttatgt gccattcatc attcacctgt atgtcatagg aatagagcag
    26821 ccccaggttc atagtgctca ggtcacccga gccggcaacg tcataagttg caaggaatcc
    26881 gattcccgag ttgaaattcg gtaccgcttt gtcgaatgaa atgctgtagg tgtggaatac
    26941 tccgggaatg gaaggccact ggttacgcca gttgatagca aggcggtagt cttccgttgc
    27001 tccggcaaaa gagggcgcca ggtacaactg gttggcataa aactgcgaga actgcgggtc
    27061 ctgactattc gcaataagcg agaaggctag aagaagacct gccagatatt gagctcgtaa
    27121 tgacaaaccg gatccgtatt tagttacaca aagtagctaa ttatacggct aaactcccgg
    27181 aaggttacat caaaatatat caacattttg cccataatgg ggaaaaaatt aacgatcctc
    27241 ctgcaacaaa ctaaaagtca atattttaaa agtattacat ctcatgttat aaaaaatgtc
    27301 tgtctgttcc ggcaggtgcc acggaccttc cacacagata gactccggga gcgcatggtg
    27361 cgcgcgttgt agtatctgcc gataaggcca ccctcatttc acgggtcatt gttacgtacc
    27421 ccgaaggcgg cttcaggacg gcattccagg aagacaagga aagccggaaa atggctgcaa
    27481 gaattgatat ttttcacaaa acatggaaaa aatgcattaa atcatggtat gcttctgata
    27541 taggtcattg aaataatggc agaatcagga gtatttttgg tagttgaatt ctaacgtgaa
    27601 agaaattcct atgacaagaa tattaaagca tcctatactt gatattccct gtaacaagga
    27661 gaccagcttt aaatataacg gaaccgtggt gaaagggatg gagggtttta ctatagctgc
    27721 tgcattgcac caggcgggtt atcctgtcca tacgcacagc cttgacaacc gcaacaggag
    27781 cctggagtgc ggtataggca agtgcggagc gtgcgagatg ctcgttgacg gcgtggtcag
    27841 gcgtatatgc atcaccccgg ttgacggtgt aaaggaggta gccgagatac ctgcaggata
    27901 ccattcagca acagcagtga aggccaaagg tgagacgcgc aaggtttacc gcaccaccgt
    27961 ggcaattgcc ggagccggtc cggcagg
//
LOCUS       DAOPBK010000007        25436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2099_length_25436_cov_5.803554, whole genome shotgun sequence.
ACCESSION   DAOPBK010000007 DAOPBK010000000
VERSION     DAOPBK010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2099_length_25436_cov_5.803554"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PLA17_01015"
     CDS             complement(<1..161)
                     /locus_tag="PLA17_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPK84022.1"
                     /translation="MSPRPKRLRRVSNPPVISGFKPYGGLAKAGKPQSVFLHLEEYEV
                     LRLCDYENL"
     gene            complement(444..1526)
                     /locus_tag="PLA17_01020"
     CDS             complement(444..1526)
                     /locus_tag="PLA17_01020"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HPK84023.1"
                     /translation="MKNQALLMIVTLAAFISSCNMRENIFIASGYSRDGVADVMLCRL
                     DGNGSIDKISEISVGDNPSYFTFGRGGLIYLVNEVDTFNMKAGGGITTLRYDKNSKSL
                     EKVSSLNQGGGGPCHIEVSADGKYLITANYGSGSVSVVKLNNGGIPDKVTDVIFYGEK
                     SRPHMTIYNPRLRTYYISDLGLDRIHQLKLDTALGLLMNADIAYFSTESGSGPRHMAI
                     DRPPANLYVINELNSSLSVYNILSDTVTAKQTVSTLPEGYAEKSYCADIHLLRNGKKI
                     YGSNRGHNSIVTFSVGIDGTLSGQTLNDCAGNWPRNFAVSPSGRFFLVANQRSDEISV
                     VHEAGNSDEAVSSLTLNAPSCVRFFK"
     gene            complement(1601..4150)
                     /locus_tag="PLA17_01025"
     CDS             complement(1601..4150)
                     /locus_tag="PLA17_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPK84024.1"
                     /translation="MKVISVLSFALLLAALLNYVSGQDLKLNELEYFERQGVNVLVYS
                     NQFTGGFNDEKNAGIELIHHGVRTAQGGAVRLSATPEQWDLVPAMPSRKVDPASKSIE
                     VALRYPDYDFDSRVVVTAKGKGVEISVYLDKPVPDALAGNAGFNLEFLPSQYWNKAYL
                     ADGRYNRFPRYVAGNSVTKPNSQKPKQFKGYVTSDDRGTGRFIDPLPLETGRTFILAP
                     DDPERLVKITSQDADLMLFDGRTLAQNGWFVVRSLLPAGKTGKVLTWTVEPNAIKGWI
                     REPNIGFSQVGYLPSQPKVSVIELDKKDKPLAKASLCRVSEDGSATRVFSGNITPWGD
                     YFKYHYVKFDFSSVTTPGIYYIQYGKHRTNNFLIEESVYDRITDATSDVWIPIHMNHM
                     FVNEAYRVWHGEPFREGYLQAPPNTDHFDLHWQGPTTDTRFKALELIPGLNIGGFFDA
                     GDFDIETGSNIMVVQNFVRIWEYFKPFRDQTFIDQKQRYVDLHRPDGTPDILQFIEHG
                     TLNLVAQAEIIGHMAQTLSNSVLDNYHHLGDAASITDGLPYNPDLGPYETAADGRSSG
                     VRDDMWAFTSRNPGLDLSAATVFAAASRALKGYNDDLSERALRQSKRLLEEATALIAD
                     RPGDNRTWGGGPATPGATLELFISTGEEQYRDKFLELLWPSLDRFVSFSLLTALDAIP
                     YIDAAYREKLRPYVVKYREYIEKLESDNPYGVPIGLGNWAGAGEVLNFGTTVCFASKY
                     FPDIIEADHAFKTANWLFGCHPYHNYSFVAAVGAARPKAVFYGNNRADFSFIPGNVAP
                     GVLFRKPDHFENYDDWPFLWGQNEGTIAGNTSYLIFGSAFRDLMNLIKPGD"
     gene            complement(4196..5647)
                     /locus_tag="PLA17_01030"
     CDS             complement(4196..5647)
                     /locus_tag="PLA17_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPK84025.1"
                     /translation="MKTKIIELSAIALISVCFAATATAQSGKPFIHDPSTIMECDGKY
                     YTFGTGGGGLISEDGWIWNSGAVRPGGGAAPDAMKIGDRYLIVYGATGGGLGGGHNGR
                     ILTMWNKTLDPQSPDFQYSEPILVAQSDGIEDNDAIDPGLLLDPNDGRLWCSYGTYFG
                     FIRLIELDPQTGKRVEGNKAIDIAIDCEATDLEYRDGWYYLLGTHGTCCDGANSTYNI
                     VVGRSRSVTGPYLDNMGRDMLRGGGKMVLSAGGRLIGPGHFGRLILEEGVEKMSCHYE
                     ADLDQSGRSVLGIRPLLWKDGWPVAGDIFREGTYEIESVRRGYALELTVDFIRMPGRM
                     RGFRPDPDEPIISIPSQELADVIHTWPAGEIDVRIGDYMSRPHQQWTITAVPDAGGYL
                     GGPYYRIVIAGTNRALAATADAEVITVPEFTGAPEQLWRIDQLTDGTYRIMPKVVPGS
                     DENLVLVSVGDSTPSLGRFDMNSDNSKWNFRSH"
     gene            complement(5702..6340)
                     /locus_tag="PLA17_01035"
     CDS             complement(5702..6340)
                     /locus_tag="PLA17_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPK84026.1"
                     /translation="MDDPTKLDTVRSIAGYFTPVTKSKKSPDADGFIQRWLLLEPISK
                     PNFTNTVFTDSYLRKAFDTLYFPDQFTILPKNGEKVRVGDNELTWHALDSKNFNVKLF
                     RFAYGLHKQVYGVIFWAVTIVNSPEEVKNVRMAVGSNSASMWWLNGEEAVLLSGDRRM
                     VMDDCVSPRLTLNKGKNIIRGVVINGPGMSDFCVRFLDENGEPVRNLTITGK"
     gene            6700..10698
                     /locus_tag="PLA17_01040"
     CDS             6700..10698
                     /locus_tag="PLA17_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPK84027.1"
                     /translation="MKRLLIILSLTLFLNPADGRPYYFRQYTNDNGLSSNTVMAGIQD
                     RRGFMWFATKEGLNRFDGTQFRIYSHNQTASNSLPNNFILALCEDNEGWIWIGTARGI
                     CYYLPDNDYFGTIENDKYQIDMAVVDIAADDNNNIWLATFDGTYKYNKQTGQLTYYPG
                     SEYFLPNRFTLTNAGDLWISADDGSIYEYNPRTDDFTGFRILTDNEITSVTLGEIVEA
                     GNYGFVVATNTAGLRLFEPNSGKVTTLFPQDNRLRNEIIINTIQLFENKNIWIGSESG
                     IHIYNLETGFVKSLQMVPTDPYSLSNNAIRFLYRDREGGIWAGTFYGGVNYLPQVTKP
                     FEKFYPTGLPGSLTGNVVREIRADSYGNIWIGTEDAGLIRFDPKSAVFYSYYGNSPSR
                     VNSRNTQGLLVDNDMLWVATYDNGIYVLNLETEKIRHHFTVKDPDLNLKTNSFITFLK
                     TADGTVYAGSFSGLYRFSRETSAFTLLDSVAPGAFIHALYEDTRGTIWIGTYGRGLFR
                     YNAKSHVCEQVKSDAGDYSNISNDYITSLYEDKDHNIWFTTEGNGFSYIDPATSAITR
                     FIPGRDVEFAIYCAILQDSHGQMWITSTRGLLRYDPPTGKFTIYTRYEGLLGNTFSYN
                     SAYQDKNGRLYFGMVNGLLTFNHADIKESTYNPPVYFTNLIVNGKEYLSNPAGSPDSK
                     SILVTDRISLKHNQSSLNIEFVSPGFTSPGLTKYRYMMVGADPDWTEMSGDHKVNYAN
                     LAPGDYRFRVIASTNGDIWSNTEASMRIRISPPWWFSLPAYIFYLITAAIIGYFLIIT
                     YLKRKAAENQRKIEAIEAGMEREILNAKINFFTNITHEVRTPLTLIKGPLDRILKAGS
                     KNLDDHEENLRIIRKNTDRLLTLTNQLLDFRKTEKEMLRLNFIRTDLYELVESVFNLF
                     LPYGEEKQISVRLNSPVNHYELAIDREAIIKILSNLLSNAIKFAGSSVDIFLEPGSEK
                     DGAVRIRVTNDGNLIPGELAEKIFEPFYQLNSNKPGEKGTGLGLSLARAIAELHHGKL
                     YLDTSVTDRNSFVLELPKFQEETISMTEIAEGDANLAENKEFEVFSSPAENSQNLLLV
                     EDEVEMSRFIARELSGEYNVIVTHNGEEALKALRKFNIILVVSDVIMPVMNGYELCRE
                     IKSTMEFSHIPVILLTASVHVNAKIEGLDSGADAYIEKPFSTEHLMAQILNLIRNRRR
                     EQQNFINSPLAHFRSVALNKTDEDFLKKLNSILLDNIAETDLSVEKISELMGISISTL
                     YRKVKALTDLNSVEYIRLTRLKKAAELLAEGNFRINEISYLVGFSSPSYFATSFQKQF
                     GISPSQFARKIK"
     gene            11097..12311
                     /locus_tag="PLA17_01045"
     CDS             11097..12311
                     /locus_tag="PLA17_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPK84028.1"
                     /translation="MMKKTFAICLLICLSAGLTCVAQSGQASNVFNADTAHASPYNLS
                     GASYPRIEADSRVTFRFNAPDAQKVQVSIVNVAYDMIKGSDGVWTYTSEPQDKGYHNY
                     WMIVDGAVVVDPATNAFMGYGHMCNGFEIPDPDGGFYQLKDVPHGNVLIKNYYSEVAG
                     SWRHIFVYTPPEYDQNIKARYPVLYLQHGGGEDERVWIEMGRTNLILDNLIAEGKIKP
                     FIVVMETSAVGPQFPMLQRPATAPAATPAPGAAQQATQAPGAPVTTVRPRFSFDKYAE
                     LMIRDLIPWVDKNFRTIPDREHRAMSGLSMGAMCTKSVTLAHPETFSYIGLFSGGSIA
                     PDEIRDKSDVKHVFMSYGSRERGSEGVAAAAEALNQAGIKSTSYVSPLTGHEWQSWRR
                     SLYEFAQLLFKE"
     gene            12371..13558
                     /locus_tag="PLA17_01050"
     CDS             12371..13558
                     /locus_tag="PLA17_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPK84029.1"
                     /translation="MNYISLILLSAALATGILCSGQDVKQTVVEDFKPSTLNQPGKQY
                     PQVNSRGRVRASISAPQALRVQLDIGGKKYDLRKDDKGVWTGESDPQDEGFHYYQLVI
                     DGAQVPDPGSMYFFGAGRWGSGVEIPASDQDFYSLKNVPHGQVREHLYFSEITRSWRR
                     CFVYSPPGYESNLSARYPVLYLQHGMGEDETGWSVQGRANLILDNLIAEKKAVPMIIV
                     MDRGYATRANQPSSPGAGRTEAGPTVFTAFEEVLIKEIIPMIDASYRTVADRSHRAMA
                     GLSMGANQTIQITMNNLDLFSYIGGFSGTSNYPGTAEIDPGKFMNGKFSDAKALNEKI
                     NLFWLSLGTKEPDPFPGSVGAFRRMLEKSGVKYVYYESPGTAHEWLTWRRSLYQFAPL
                     LFR"
     gene            13604..15523
                     /locus_tag="PLA17_01055"
     CDS             13604..15523
                     /locus_tag="PLA17_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPK84030.1"
                     /translation="MKNLITILALLAGFALSASSQDFEKHAPAGFDSVRAEIPHGKID
                     TIKYYSKTVGTSRKALIYTPPSYSKRNRYPVLYLLHGIGGDEKEWLNGGRPQVILDNL
                     YADGKIEPMIVVMPNGRAMKDDRAGGNIMAPDKVEAFATFEKDLLNDLIPFIEKKYPV
                     IRDREHRAIAGLSMGGGQALNFGLGNPDRFAWVGGFSSAPNTKPPEQLVPDPEKAKKE
                     LKVLWISCGDNDGLLAFSRRTHEYLAANGVPHIYYIEPGVHDFKVWKNGLYMFSQLLF
                     KPVDKTRFNKYSPLGTPAPSNVRSAKYPQIMPDGSALFRLKAPGAQKIQLDLGKKYEM
                     ARNADGTWEVKTDSLTEGFHYYSLIVDGLTVADPASESFYGMGRMASGIEVPFSGDGY
                     YAVKDVPHGEIAIKRYFSTVFNTWRQFYIYKPAGYDLDSDRRYPVLYILHGGGEDERG
                     WAIQGKTDLILDNLIAADMAKPMLIVMPDGNAGGPMGFGEMALAMFERELTQCIIPFV
                     EKNYRAETTPGNRALAGLSMGGLQTLYVGINNAAMFPYLGVFSSGWMAPAHNELAGKQ
                     YEFIKNNPEKISPLRLFWISMGGKEDIAWNNCQEMMKKLDELNVEYTYSEYPGGHTWP
                     VWRNNLYNFAQLLFR"
     gene            15650..16810
                     /locus_tag="PLA17_01060"
     CDS             15650..16810
                     /locus_tag="PLA17_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPK84031.1"
                     /translation="MKKIALFLLTMMICGITSAQFAGGMFGQRQEPLPEGYKPASTNT
                     FLAQYPAVNAQTRQAIFKVVAPHADNVALDLAGKKYSMTKDEKGVWTCTSDPQVVGFH
                     YYAILIDSVPVMDRNTNSYFGSNWQSSGIEIPEGPEGDYYRFNKDIPHGHVRSLYYWS
                     EINGMERHCNVYVPAEYESNPDKKYPVMYLLHGWGEDENGWSNQGHMANIMDGLIHAG
                     KAVPMIVVMDCGDIKTNSDVRKASTNDVTQIYIKDLMPFIERTFRTKNDRQNRAMAGL
                     SRGGFQTTMTVFANLDKFAWMGTFSGFFSRPGDNITEAFNGVFKDPAAFDKQMNLLFI
                     STGTEERTPKEAVDALKAHGIKNIVYHESQGTAHEWLTWRRALNEFAPLLFK"
     gene            17047..17823
                     /locus_tag="PLA17_01065"
                     /pseudo
     CDS             17047..17823
                     /locus_tag="PLA17_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
     gene            17903..19477
                     /locus_tag="PLA17_01070"
     CDS             17903..19477
                     /locus_tag="PLA17_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HPK84032.1"
                     /translation="MKRNCFILIVLINLLHCITVTGQKDHARNPVIWADVPDMSIIRV
                     GNTWYMTSTTMHMSPGVPVMKSNDLVNWELVNYAYNTLGDNDALNLIDGRNAYGRGSW
                     ASCIRYHNETFYVSTFSSTTNKTYIWSTKDIEKGPWKEISFTPSFHDHTLVFDDDGRV
                     YLIYGNKKLTLVELNADLSGVRPGGINQVIIEDASAPSGTDSGLGEGSQLFRMNGRYY
                     LFNITWPRGGMRTVVVHRADRLNGPWEGKLIFQDLGVAQGGMTDTPDGRWYAYLFRDY
                     GSVGRIPYIVPMRWEDGWPVIGIDGKVPEVLDLPPGKGLIPGIVAPDEFKRKKGDPPL
                     PLVWQWNHNPDNALWSVTQRKGFLRLTAGRTDTLFTQARNTLTQRTLGPECSGSTLID
                     VSGMKEGDFAGLALLQKKYGIVGVKYSNGIRSVVMVNAQSGSPEEVESIPLTRKKVYL
                     KAECDFTDRRDIAYFYYSLDGKTWKPIGNQLKMEYSMPHFMGYRYGLFNYATREPGGF
                     VDFDWFRITNERTEGE"
     gene            19455..20843
                     /locus_tag="PLA17_01075"
     CDS             19455..20843
                     /locus_tag="PLA17_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPK84033.1"
                     /translation="MKELKVNEKQLKNITIKTMKNLRLFAAVLVLLISTLASSQNPVI
                     RDQFTADPTARVFNGKMYLFPSHDIPAPESFPRKDWFCMADYHVFSSSDLTDWTDHGV
                     IVSQENVAWVNPTSFSMWAPDCIERNGKYYFYFPANVKSGRGFGIGVAIADKPEGPYT
                     PLPEPIANVMGIDPSVFIDKDGQAYIYYSLGRIFAAKLKDNMTELASEPVVVGDLPTK
                     GLVEGPFFFERNGIYYMTYPHVENTIERLEYAIGDNPLGPFRVTGVIMDETPMGTWTN
                     HHSLVQYNDQWYLFYHNSAYSPNFDKNRSVCIDSLFFNDDGTIRKVIPTNRGVGITGA
                     QTTIEIDRFSQVSPTGVNVALIDTTNTFLGWSAELKGQNSWISYNAVDFGRKKLKSVR
                     VNASSPAGGIIHIRLDNPDGLLLAEIEIPESTALRTVDAKLKKYEKGIHNLFVVLKDE
                     NPVNIDWVQFRR"
     gene            20895..21020
                     /locus_tag="PLA17_01080"
     CDS             20895..21020
                     /locus_tag="PLA17_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84034.1"
                     /translation="MKSLIIGSFAALPVLSACSSGVKTIPEAKDIIWYDKPAGEA"
     gene            complement(21116..22822)
                     /locus_tag="PLA17_01085"
     CDS             complement(21116..22822)
                     /locus_tag="PLA17_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPK84035.1"
                     /translation="MKKSLIIISLILASCSGEMIKQERTYCNPLNLNYRFQHTDSVSY
                     REAADPAMIRYKDKYILFVSHSGGYWYSDDLHEWTFLPVGSLPIEDYAPDAIAINDTV
                     WFAASSTERKPFYYTTDPFSDSWKPLNDTLPFAVWDPHFFADDDGQRYLYWGCSNFLP
                     INGVMLNSGMQAVTEPRILIEHNSDIYGWEVPGEQNELTSNGWNEGAWLTRHNNRYYL
                     QYAAPGTEYRTYADGVYTSASPLGPFEYESYSPFSYKPGGFAGGAGHGSTFQDKYGNY
                     WHIATMSISIRHMFERRLGLFPAAFDEEGVLRTFTAFGDYPAILPERKVDFEKENTFT
                     GWMLLSYKKNAESSSEVDGFPVSNAFDEDIRTWWSAATGDSGEWLSVELDDASTVTAI
                     QVNFADHESTLKPDSRDIFYRYRILASHDGKSWDVIADKSNNSTDACHDYIQLENPVR
                     TRFIKIENVSVPDGKFSLYDLRVFGTREGDAPAEIRDFTVSRNETDTRKALVEWPACD
                     QATGYVVNYGTDPGKLYTSVMVYDTGSLMLTGLNRDVKYYFSVDAFNESGITRGTVVV
                     QR"
     gene            23095..24600
                     /locus_tag="PLA17_01090"
     CDS             23095..24600
                     /locus_tag="PLA17_01090"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPK84036.1"
                     /translation="MKKLIVLFGCMVLSGILSGNLMSQGNAVIKIDFDRQTGKVDPNI
                     YGAFVEPIRNVVYGSIYDPASPFADENGFRKDFVELVRELKIPVVRWPGGNYVSGYNW
                     EDGIGPKEERPVRLDLAWNQIESNHMGTDEYVQLCKLIGAENFICINAGTGTLDQARH
                     WVEYCNREKGTWYSDLRRKYGNEEPFKVKYWALGNEIDGPWQMGQKSAEDYVKFALEA
                     GKLMQLVDKDIKLIASGASNYKPDNGWIDWNDYVLTHMTGTIDYLSVHRYATEALGND
                     RSFSGMMCLGLDLDQKIETVKALILKAMTKTGSTRPVYISFDEWAGGFGNNITVSLMV
                     AQHLNSFIRHADIVKMANMTMLSNLVGYSPEGVYKNATFMAFRLFSNNCFGTSLDVST
                     TCEKYSNSIFKEIPYLDVTAVLNDEAGNLVINVINRHETKAIITDIVLQTGEFTGNAK
                     VNEVNGKITDPGNPGTAEGVNSTSYDIRFKGNTIKYSFPAHSFTQIIIPLK"
     gene            24961..>25436
                     /locus_tag="PLA17_01095"
     CDS             24961..>25436
                     /locus_tag="PLA17_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPK84037.1"
                     /translation="MVLFTFTLSMFGSNAYCLGADDLNLNSDITLQQLRVAGVVRDTD
                     GQPIPGVTVQVEGTTTGAFTDVNGRYTVDVPGPNAVLAFSFVGYTQVTVPVNGRNVID
                     VTLEEATEALDEIVVVGYGTVRKSDVTGAVASVRADAIKNVSTTDAAAAIQGKAPGV"
BASE COUNT         6971 a         6050 c         6008 g         6407 t
ORIGIN      
        1 ttgagattct cataatcaca gagcctgaga acctcatact cctcaaggtg cagaaagact
       61 gattgcggct tgccagcctt cgctagcccg ccatagggct tgaacccgga gataaccgga
      121 ggattgctga ctcttcttaa tctttttggg cgtggggaca ctttttgatc atttgttcat
      181 tacaaatata atgaacatat gttcataata aaaatatcat aaggtttttt gtggattatt
      241 attataacag atttgcaact gctattcatt ccaacagtag ctgctattca ttccggccat
      301 agttgttatt aattacggtc gaaacagctc tgcattccgg acgcaactgc tcctgattcc
      361 ggtcataaat gctctgcatt ccgaacgcag atgctattta tttctgtggc agctggctct
      421 gaatatggga tatgtgagcc tctctactta aagaatctga cacatgaagg ggcattgagc
      481 gtcagagatg agacagcttc atcactgtta cccgcctcat gtaccacaga aatctcatcc
      541 gatctctggt tagcaaccag gaaaaacctg cctgacggcg ataccgcgaa attgcgaggc
      601 cagttaccgg cgcagtcgtt gagagtctga cccgacagtg ttccgtcaat ccccacactg
      661 aaggtcacga tgctgttatg tcccctgttg gaaccataga ttttcttccc gttacgcaag
      721 aggtgtatgt cagcacaata acttttctct gcataccctt caggaagggt gctgacagtc
      781 tgttttgcag taacggtatc tgacaggata ttgtaaactg acaatgagga gttgagctca
      841 ttgattacat agaggtttgc cgggggtctg tctattgcca tatgcctcgg gccgcttccg
      901 gactcagtgc tgaagtaagc tatatctgca ttcataagaa gccccagagc ggtatccagc
      961 ttcagctgat gaatcctgtc aagacccagg tcggatatat aatatgtgcg aagcctggga
     1021 ttataaatgg tcatgtgggg gcgcgacttc tcaccgtaaa agataacatc cgtgacctta
     1081 tctggtatgc ccccgttatt cagcttaacc acggagacag aaccgctgcc gtagttggct
     1141 gtgatgaggt atttgccatc agctgaaacc tctatatggc atggtccgcc acctccctgg
     1201 ttcaatgaac tgactttttc aaggcttttg ctattcttat cataacgtaa ggttgtgatt
     1261 cctcccccgg ctttcatgtt aaaagtgtcg acctcattga caaggtatat caggcctcct
     1321 cttccgaagg taaaatatga agggttatcc ccgacggaga tctccgatat cttatcaatg
     1381 ctgccgttgc cgtccagccg gcatagcatc acatcagcaa caccatcgcg tgaatagccg
     1441 gatgctatga aaatgttctc cctcatatta caggaagaga tgaacgctgc aagtgtcaca
     1501 atcattaaca aagcctgatt cttcattgga ttgcctgtat atcattattc tcgtttgtct
     1561 gcctctatag taccggcctg accggatttt gctgccggct ttaatcacca ggcttaataa
     1621 gattcatcag atccctgaaa gctgatccga aaatcaggta actggtattg cctgcaatgg
     1681 tcccctcgtt ctgtccccac aggaatggcc agtcgtcgta gttttcaaag tgatcaggtt
     1741 ttctgaacag tacccccggc gcgacattac ccggaatgaa agagaaatca gccctgttgt
     1801 taccgtagaa gacagctttg ggacgtgcgg cacccactgc tgcaacaaat gaataattgt
     1861 ggtacggatg gcatccaaac agccagttgg cggtcttgaa tgcatgatca gcctcaatga
     1921 tatcagggaa atatttgctg gcaaagcaaa ctgtagtgcc aaaattcagc acttctcccg
     1981 ctcctgccca gttgccaagg cctataggca ctccgtaagg attatcactt tcaagctttt
     2041 cgatgtattc cctgtacttt acaacatagg ggcggagctt ctccctgtag gcggcatcta
     2101 tataaggtat ggcatccagg gctgtcagga ggctgaagct gacgaaacgg tcaagtgaag
     2161 gccataaaag ttccaggaat ttgtccctgt actgctcctc tcctgttgaa atgaacaact
     2221 caagagtggc accgggagtt gccggacctc ctccccatgt gcggttatcc ccgggtctgt
     2281 cggcaatcag agccgtcgcc tcttccagaa gtcgtttcga ctgcctgaga gctctctccg
     2341 agaggtcatc attgtatccc ttcaaagccc ggcttgccgc ggcaaagacc gttgcggctg
     2401 aaaggtccag accgggattg cggcttgtaa atgcccacat gtcatccctg actccgctcg
     2461 agcggccgtc agcagctgtc tcatacgggc ccaggtcagg attatagggc aggccgtcag
     2521 ttattgaggc tgcatcaccc agatgatggt aattgtcaag aacagagttc gagagcgtct
     2581 gcgccatatg gccgataatc tctgcctgtg ctaccaggtt cagtgtccca tgctctatga
     2641 actgtaatat gtccggtgtc ccgtcagggc ggtgaagatc tacgtaccgc tgtttctgat
     2701 caataaaggt ctgatcacga aagggtttga aatactccca tattcttaca aagttctgca
     2761 ccaccattat gtttgatccg gtctcgatat caaaatcgcc ggcatcgaaa aaacctccga
     2821 tgtttagtcc gggaatcagt tccagcgcct tgaacctggt atcggttgtc gggccctgcc
     2881 aatgcaggtc aaagtggtca gtgtttggcg gagcctggag ataaccttct ctgaacggct
     2941 cgccatgcca gaccctgtat gcctcgttta cgaacatatg attcatgtgt atcggaatcc
     3001 atacatcgga cgtggcatcg gtaatcctgt cgtaaacgct ctcctcaatc aggaagttat
     3061 ttgtcctgtg cttcccatat tgaatatagt aaataccagg ggtcgtgacc gaagaaaaat
     3121 cgaatttcac atagtggtac ttgaaatagt caccccaggg agtgatattg ccgctgaata
     3181 ccctggttgc actaccgtct tcgcttaccc tgcagagtga tgcttttgcc agtggcttgt
     3241 ctttcttgtc aagctcaata acggagactt tgggctgtga cgggaggtag cccacctgcg
     3301 aaaagccgat gtttggctct cttatccagc ctttgattgc attgggttca actgtccagg
     3361 tcagtacctt tccggttttt cctgccggaa ggaggctgcg tacgacgaac cagccatttt
     3421 gagcgagcgt acgaccatcg aatagcatca ggtctgcatc ctgtgaagtg attttcacca
     3481 ggcgctcagg gtcatcaggt gcaagaataa aagtgcggcc tgtttcaagc ggcagcggat
     3541 caatgaacct gccggttccc ctgtcatcgg atgtaacata ccctttgaac tgcttaggct
     3601 tctggctatt gggtttagtg acactgttgc ctgccacata ccgggggaac cggttatagc
     3661 gcccgtcagc cagataagcc ttgttccagt attgggaggg aagaaactca agattaaacc
     3721 ctgcatttcc tgcaagtgca tcaggaacgg gtttgtcaag ataaacagat atctcaacgc
     3781 ccttgccttt ggcggtgaca actacccttg aatcaaaatc gtaatcagga tacctcaatg
     3841 caacttctat tgatttcgat gcaggatcca ctttccgcga cggcattgca ggcaccagat
     3901 cccactgctc cggggtggct gaaagcctca cagcaccacc ctgtgccgtt ctgacaccat
     3961 gatgaatcaa ttctattccg gcgttcttct catcgttgaa acctccggta aactggttac
     4021 tgtataccag gacattgaca ccctgcctct cgaaatactc aagctcattg agtttcagat
     4081 cctgcccgga aacgtagttg agcaacgcgg ccaaaagcag tgcgaatgat aaaacagata
     4141 ttaccttcat gcttaacaat atcaaatcag atagaggtat aaatttaaaa cctgctcagt
     4201 gacttctgaa attccatttt gaattgtcac tgttcatgtc aaatctgcca agtgaaggcg
     4261 tactgtcacc cacagataca aggaccaggt tctcgtctga accagggacc acctttggca
     4321 taatcctgta tgtgccgtca gtcagctgat ctattcgcca taactgctcc ggtgcacccg
     4381 tgaattcagg aacagtaatt acctctgcat cagcagttgc tgccagcgca cggtttgtcc
     4441 ctgctatgac aatcctgtaa tacgggcctc caagataccc tcctgcgtca ggcactgccg
     4501 taatagtcca ttgctgatgc gggcggctca tgtaatctcc gatccttacg tcaatctctc
     4561 cggccggcca tgtgtgaatt acatcggcca gttcctgtga aggaatggaa attatcggtt
     4621 catcagggtc gggacgaaac ccgcgcatcc ttccgggcat tcttataaaa tccactgtca
     4681 gttccaaagc atatcctctt ctcacagact caatttcata tgttccttcc ctgaagatat
     4741 ccccggcaac aggccagcca tctttccaca gcaacggacg gatgcccagt acgctgcggc
     4801 cactctggtc aaggtccgct tcataatggc atgacatctt ttcgaccccc tcttcgagaa
     4861 tcagccgtcc gaaatggccc ggtccgatca gcctgcctcc tgcagacagc accattttac
     4921 ctccgcctct gagcatgtct cttcccatat tgtcgaggta tgggccggtc acactcctgg
     4981 agcgtccgac aacaatatta tatgtcgagt ttgcaccatc acagcatgtt ccgtgcgtgc
     5041 caaggaggta ataccacccg tcacgatact ccaggtctgt tgcttcacag tcaatggcaa
     5101 tatcaatggc tttattcccc tccacgcgct ttcctgtctg cggatcaagt tctatgagac
     5161 ggataaaccc gaaataggtt ccgtaggagc accagagccg tccatcattc ggatcaagga
     5221 gaaggcccgg atcgattgca tcattatctt caatgccgtc ggactgggca accaggatgg
     5281 gttccgaata ctggaaatcg ggagattgcg ggtcgagcgt cttattccac attgtaagta
     5341 ttctcccgtt atgtccgcca cccaggccac cgcctgtagc gccataaact ataagataac
     5401 gatcgcctat tttcattgca tccggtgcgg ctccgcctcc cggccttact gcaccgctgt
     5461 tccatatcca gccatcttcc gatatcaaac cgcctccgcc tgttccaaag gtgtaatact
     5521 tcccgtcaca ctccatgatg gttgacggat catgtatgaa aggtttgcca gactgtgctg
     5581 ttgctgttgc cgcaaaacaa actgatatca gggctattgc actcaattct ataatttttg
     5641 ttttcatcat aatacgatat tgtttttaaa ggaatgatgc gatccacatg attgaatgac
     5701 actatttacc ggtaatggtc aggtttctta ccggttctcc attctcatcc aggaaacgaa
     5761 cacagaaatc actcattcca gggccattga ttacaactcc ccggattata tttttcccct
     5821 tgttaagcgt caggcgggga gacacacaat catccataac cattcgcctg tcgccggaaa
     5881 gcaatactgc ttcctccccg ttcagccacc acatggatgc ggaatttgac cctactgcca
     5941 tcctcacgtt cttaacttcc tccggactgt taacaattgt taccgcccag aagatcacac
     6001 catacacctg tttatgcaat ccataggcaa aacggaacag tttaacattg aagtttttgc
     6061 tgtccaatgc atgccatgta agctcgttgt ctccaactct taccttttca ccattctttg
     6121 gaagaattgt aaactgatcc gggaaataga gggtatcaaa cgctttcctg aggtaactgt
     6181 ctgtaaaaac agtattggta aagtttggct tgctgattgg ctcaagcagc aaccagcgct
     6241 gaatgaaacc atcagcatca ggtgactttt tcgattttgt taccggtgta aagtaaccag
     6301 caatgcttct tacagtatca agttttgtgg gatcgtccat ctgacgaaag ggtctctcgg
     6361 gttgtgcgtg caacaccggc ccggccagaa ataataatcc aaccccgaat agtaatgctt
     6421 tggttttaat gctcataacg tatctttaaa atgtttgtgt ttatatcggt tcaaaatatc
     6481 aattattcat ctctgcattg tcccggacaa ataaccccgg aaattaaatc gctaatattc
     6541 tttcacttaa aagccagcac cagattccgc tgcatcttgc gcaagaatac gaaagtaaca
     6601 aaattttatg tttgcaaagt gccggcactc ttttccggaa gcagttcggg gatattttgc
     6661 taactttcgt accccggttt ttttgtaaaa tattgacagg tgaagagact tctgataata
     6721 ttatctctca cattattcct gaatcctgct gatggcaggc cgtattactt cagacagtac
     6781 acaaatgaca acggcctgtc gagcaataca gtgatggcag gcattcagga ccggagaggc
     6841 tttatgtggt ttgccacgaa ggaaggcctt aaccgttttg acggcactca gttcaggatc
     6901 tacagccata accagacagc atcaaacagc ctcccgaaca actttatcct ggccttatgc
     6961 gaggataatg aaggatggat atggatcgga acagccaggg gtatctgtta ttatctgcct
     7021 gacaatgatt acttcggtac gattgaaaat gacaagtatc agattgacat ggctgtggtg
     7081 gacatagctg ccgatgacaa caataatatc tggcttgcca cttttgacgg aacttacaag
     7141 tacaacaagc aaaccggtca actgacatat tatccgggga gcgagtactt cctgcccaac
     7201 agattcacac tgacaaatgc aggtgatctc tggatctccg ctgatgacgg atctatttat
     7261 gaatacaatc ccagaactga tgacttcacc ggcttcagaa tccttactga caatgagatc
     7321 acctcggtta cccttggtga gattgtggag gccggtaatt acggatttgt ggttgcaaca
     7381 aataccgcag gcctcaggtt gtttgaaccc aactcgggta aggtaaccac tctgttcccg
     7441 caagacaacc ggttgcgcaa cgaaattata atcaacacta tacagctatt tgaaaataaa
     7501 aacatctgga taggatcaga gtcaggcatt catatctata atcttgaaac ggggtttgtg
     7561 aaatccctcc agatggtacc gacagatcct tactctcttt caaataacgc aatccgtttc
     7621 ctctaccgcg acagggaggg gggcatctgg gcagggacat tttatggcgg ggtcaattat
     7681 cttccacagg taaccaagcc tttcgaaaag ttttatccga ccggattacc cggttcactg
     7741 accggtaacg tggtaaggga gatcagggct gactcatacg gcaatatctg gataggtacg
     7801 gaagatgccg ggctgatcag atttgatccg aagagcgctg ttttctactc ttactacggg
     7861 aacagtccct cacgggtaaa cagccggaat acccagggat tgctggttga caatgacatg
     7921 ctatgggtcg cgacgtatga caacggcatt tacgtactga atctggagac agaaaagatc
     7981 aggcatcact tcactgtcaa agaccctgac ctgaatctga aaaccaactc tttcataact
     8041 tttctgaaga cggctgacgg aactgtctac gcgggcagct tctcagggtt gtaccggttc
     8101 agcagggaga cctctgcctt cactcttctt gattcagtag cgcctggcgc tttcatacat
     8161 gccctttatg aggataccag gggaaccatc tggataggaa catacggaag aggtttgttc
     8221 aggtataacg caaaatctca cgtttgcgaa caggtgaaat ctgatgccgg tgattacagt
     8281 aatatcagca atgattatat aacctcgtta tatgaggata aggatcataa catctggttt
     8341 actacggaag gaaacggctt cagctatatt gatccggcaa cctccgccat cacccggttc
     8401 attccgggca gggatgttga gtttgcaata tactgtgcca tccttcagga cagtcatggt
     8461 cagatgtgga taacttccac caggggattg ctgcgctatg atcctccgac aggtaaattt
     8521 accatatata cccgatacga gggcctgttg gggaacacct tcagttataa ttccgcctat
     8581 caggacaaga atggcaggct ctatttcggg atggtgaacg gactgttgac attcaatcat
     8641 gcagacataa aggaaagcac atataacccc ccggtatatt tcaccaattt gattgtcaat
     8701 ggcaaagagt atctcagtaa tccggccggt tctcctgatt caaagtcaat acttgttact
     8761 gacagaatct cactgaagca taatcagtca tcgctgaaca ttgagtttgt gtcaccaggt
     8821 tttacaagcc ccggtctcac aaaatacagg tacatgatgg tgggcgctga ccctgactgg
     8881 acagagatgt caggcgacca taaagtaaat tacgcaaatc tggctcccgg agattacaga
     8941 ttcagggtca ttgcatcaac caacggagat atatggagca ataccgaagc cagcatgagg
     9001 atcaggatat ctcccccatg gtggttctct ttgcctgctt acatctttta cctgatcact
     9061 gctgccatta tcggctactt tctgataatc acctatttga aaagaaaagc tgctgaaaat
     9121 caaaggaaaa tagaagccat tgaggccggg atggagaggg aaatactaaa tgccaagatc
     9181 aacttcttta ccaacataac ccatgaagtc aggactccct tgacgctcat caagggtccg
     9241 ctcgacagaa tcttaaaggc tggttcaaaa aacctggatg atcatgagga gaatctccgg
     9301 atcatcagaa agaatactga caggcttctg actcttacca accagttgct tgatttcagg
     9361 aagacagaga aggaaatgct gagacttaat ttcatcagaa ctgacctgta cgagcttgtg
     9421 gaatcagtat ttaacctgtt cctgccatac ggcgaagaaa agcagatatc tgtgaggttg
     9481 aattcgccgg tgaatcatta cgagcttgcc attgacaggg aagcaataat aaagatttta
     9541 agcaatctgc tctcgaatgc aataaagttt gccggatcat ctgtcgacat ctttcttgag
     9601 ccgggctcag agaaggatgg tgccgtgaga atacgggtaa ccaatgacgg caatctgatc
     9661 cccggtgagt tggccgaaaa gatctttgaa cctttttacc agcttaactc caacaaaccg
     9721 ggtgaaaagg gcactggtct gggattgtct ctcgcccgcg caatagctga gctgcaccat
     9781 gggaaactct atcttgacac ttcagtgacc gacagaaact cgtttgtcct tgaactaccc
     9841 aaattccagg aagaaacgat cagtatgaca gaaattgcag aaggagacgc aaaccttgct
     9901 gaaaacaaag agtttgaggt attcagctcc cctgcagaaa acagtcagaa cctgctcctt
     9961 gtggaagacg aggttgagat gagcagattc atagccaggg aattatcagg tgaatacaat
    10021 gtgatcgtga cccataatgg agaagaggct cttaaagcat tgaggaagtt taacatcatt
    10081 ttggttgtaa gtgatgtgat tatgccggtc atgaacggat atgaactatg ccgggagatc
    10141 aaatctacca tggagttcag ccatattccc gtcattttgc ttacagcctc tgttcacgtc
    10201 aatgcaaaga ttgagggcct tgactccggc gctgatgcct atattgaaaa accattctcc
    10261 actgagcatt taatggctca gatacttaat cttataagaa acagaaggcg ggaacagcag
    10321 aacttcatca attctccgct tgcacatttc aggtcggttg ccctgaacaa aactgatgag
    10381 gatttcctta aaaaactgaa ttccatcctc cttgacaata ttgccgaaac tgatcttagc
    10441 gtggagaaaa tatcagagtt aatgggaata agtatctcta ctctttacag gaaggtaaag
    10501 gcactcactg acctcaactc tgttgaatac atcaggctta caaggctgaa gaaggcagct
    10561 gagctgctgg cagaaggcaa tttcagaata aatgagatat catatctggt gggcttctcc
    10621 tctccttcat attttgccac aagcttccag aagcaattcg gtatttcacc ctcgcagttt
    10681 gccagaaaga taaagtaaac agtatttcaa aaccggccag cccgattatg gcatccgcca
    10741 gtgccatgac cgttttttgc attccactcc tctcaattat gcttttttaa actcttacag
    10801 ggtcaacttt aatttttcag tcactatttg cacaaaaaat gaatctgttt cagacttttt
    10861 attaggagtt ttacaccatt acgggctata aatcccggat taacttaact gaaacagatc
    10921 aactgaagaa cattgtaaaa atattgtctg aaagcctgtc ttcatttttg atccgggaat
    10981 ttacaaacct gacaggagat aataatgaaa cccatatgta tcaaatgcac aaacacctgt
    11041 tgatgtattt ttcaggcatg tgggttccgt cataccttaa aaatcaaagc tatacgatga
    11101 tgaaaaaaac atttgccatt tgtctgctga tatgcctgtc agcaggcctg acctgtgttg
    11161 cccagtcagg acaggcatcg aacgttttca atgctgacac ggctcacgcc tcgccatata
    11221 acctctccgg cgccagctac ccgcgcattg aggctgactc aagggttacg ttccgtttta
    11281 atgctcccga tgcacaaaag gtgcaggtct caatagttaa cgttgcatat gatatgatca
    11341 aagggagtga cggagtatgg acttacacca gtgagccgca ggataagggg tatcacaact
    11401 actggatgat agttgacgga gctgtggtgg ttgatccggc taccaatgca ttcatgggtt
    11461 atggccatat gtgcaacggg tttgaaatac ctgaccctga tggcggtttt tatcaactga
    11521 aggatgtgcc acacgggaat gtcctgataa agaattacta ttcggaagta gccgggtcat
    11581 ggcgccatat ctttgtttat actccaccgg aatatgatca gaatatcaaa gcccgatacc
    11641 ccgtcctcta ccttcagcat ggtggcggtg aggatgaaag ggtctggatt gagatggggc
    11701 gcacaaacct catccttgat aatctgattg cagaaggaaa gatcaagccg tttattgttg
    11761 tcatggaaac aagcgctgtg gggcctcaat tccccatgct ccagcgtcct gccactgccc
    11821 cggcagccac acctgcaccc ggagcggctc aacaggcaac gcaggctccc ggtgctcctg
    11881 tcacaactgt acgcccccgc ttttcatttg acaaatatgc cgaactgatg atcagggacc
    11941 tgatcccatg ggttgacaaa aatttccgta ccattcctga cagggagcac agggcgatgt
    12001 cgggattgtc aatgggcgcc atgtgcacca agtcagtcac cctggcgcat cccgaaacat
    12061 tttcatacat cggactcttt agcggtggct caatagcccc tgacgaaatc agagacaagt
    12121 cagatgtcaa acatgtgttc atgagctacg gcagccgtga aagaggctct gaaggggtcg
    12181 cagctgcagc agaagcgctg aatcaggcag ggataaaaag cacctcctat gtctcgcctc
    12241 ttacaggcca tgaatggcaa tcatggagaa gaagccttta tgaatttgca cagttgctgt
    12301 ttaaagagtg aagatgagga ttatttgtaa tcattcagaa ttggtaattg atgatcatta
    12361 aagcaagatg atgaattaca tatctttaat actgctgtcg gctgcactgg caaccggcat
    12421 tctctgctca ggccaggatg taaagcagac cgtcgttgaa gattttaaac cctcaaccct
    12481 gaaccagccc gggaaacagt atccacaggt aaattcccgg ggccgggtcc gtgcaagcat
    12541 atcagcacca caggccctga gagtacaact ggacatcgga ggtaaaaagt atgatctcag
    12601 aaaggacgat aaaggtgtct ggaccggtga atctgatcct caggatgagg gatttcacta
    12661 ctaccagctc gtcattgacg gagcacaggt acctgatccg ggaagcatgt acttcttcgg
    12721 agcaggccgc tggggcagcg gggtagagat accggcctca gaccaggatt tttattcgct
    12781 gaaaaatgta ccacacggac aggtaaggga gcatttgtac ttctctgaga ttacccgatc
    12841 atggagacgc tgctttgtct attcccctcc cggctatgaa agcaatctct ctgcccgtta
    12901 tccggtgctc tacctgcagc acggcatggg agaagatgaa acgggctggt cagtacaggg
    12961 cagggccaat cttattcttg ataacctgat tgctgagaaa aaggctgtgc cgatgattat
    13021 tgtaatggac agaggatatg ctacaagagc gaatcagcca tcctcacccg gtgccggaag
    13081 aacagaggca gggccaacag ttttcaccgc attcgaagag gttctgataa aggagatcat
    13141 tccgatgatt gacgccagct accggaccgt ggctgaccgc agccacaggg ccatggcagg
    13201 tctgtcaatg ggagccaacc agaccattca gataacgatg aataaccttg acctcttctc
    13261 ctacataggc ggattcagcg gaacctccaa ctatccgggt accgcggaga ttgatcccgg
    13321 caaattcatg aacgggaagt tcagtgatgc aaaagcgctg aatgaaaaga taaacctgtt
    13381 ctggctcagt ctcggaacca aagagcctga ccccttcccc ggttcggtgg gggctttccg
    13441 ccggatgctt gaaaagagcg gagtaaaata tgtctattac gagtcacccg gtacagctca
    13501 tgaatggctg acctggagaa ggagcctgta ccagttcgca cccctgcttt tcagataatg
    13561 aaagaaactt tttatgccgg ttaacattca tattcagtca gaaatgaaga atttaataac
    13621 aatccttgct ctccttgccg ggttcgccct ctcagccagt tcacaggatt ttgagaagca
    13681 tgcaccggca ggatttgact ccgtgagagc tgaaatcccc catggtaaga ttgataccat
    13741 taagtattat tcaaagacag tgggcacctc acgaaaagcc ctgatctata ctcctccctc
    13801 ctactcaaag cgcaacagat atccggttct ctacctgctt cacggcatag gtggtgatga
    13861 aaaggaatgg ctcaacggag gccggccaca ggtgatcctc gacaaccttt atgctgacgg
    13921 gaagatcgag ccgatgatcg tggtcatgcc aaacggcagg gccatgaagg acgaccgtgc
    13981 cggcggcaac ataatggcgc ctgacaaggt ggaggctttt gcaacgttcg aaaaggatct
    14041 gcttaatgat ctcattccat tcattgaaaa aaagtatccg gtgatcaggg accgtgaaca
    14101 ccgtgccata gcgggtttat ccatgggggg cgggcaggcg ctgaacttcg gtctcgggaa
    14161 tcccgacagg tttgcatggg tcggtggttt ctcatcggct ccgaatacaa agcctccgga
    14221 acagctcgtg cctgaccctg agaaggcaaa aaaggagttg aaggtgttat ggatatcatg
    14281 cggtgacaac gacggtctcc tggctttcag ccggagaaca catgaatatc ttgcggccaa
    14341 tggcgttccg cacatttatt atatcgaacc cggcgtgcat gacttcaagg tctggaaaaa
    14401 cggtttgtac atgttttcac agcttctgtt caagcctgtg gataagacca ggttcaataa
    14461 gtacagtccg ctgggcacac cggctccgtc aaatgtaagg agtgcaaagt acccccagat
    14521 aatgcctgac ggcagtgcac tgttccggct gaaggctccc ggtgcacaaa aaattcagct
    14581 tgacctggga aagaaatatg agatggccag aaacgctgac ggcacctggg aagtaaaaac
    14641 cgattcactg acagaaggat ttcattacta ttccctcata gttgacgggc tgacagtggc
    14701 tgaccctgcc agtgagtcat tttatggcat gggccggatg gccagcggga tcgaagtccc
    14761 gttcagcggt gacggttact atgctgtaaa ggatgtccct cacggggaga ttgcaatcaa
    14821 aagatatttt tcaacggtat tcaatacctg gcgacagttc tacatttaca agccggcagg
    14881 ctatgatctg gacagtgaca ggagatatcc cgttctctat atcctccacg gcggaggcga
    14941 agatgagaga gggtgggcaa tccagggcaa gaccgacctt attcttgata acctgattgc
    15001 agctgacatg gccaagccga tgttgattgt aatgcctgac gggaacgcag gcgggccgat
    15061 gggctttgga gagatggcac tcgcaatgtt cgaaagagag ctcacccaat gcatcatccc
    15121 cttcgtcgaa aagaattacc gtgcagaaac aacccccggc aaccgtgcac tggcaggtct
    15181 ctcgatgggt ggactgcaga cactgtatgt cggaatcaat aatgccgcga tgttccctta
    15241 cctgggggta ttcagctccg ggtggatggc acctgctcat aacgagctgg ccggcaaaca
    15301 atatgaattt attaaaaaca atccggaaaa gatcagtccc ctgagactct tctggatcag
    15361 tatgggaggc aaagaggata ttgcctggaa caattgccag gaaatgatga aaaaactgga
    15421 tgagctgaat gtggaatata cctacagtga atatccgggc ggacatacat ggcccgtctg
    15481 gagaaacaac ctgtataatt ttgcccagtt gctgttcagg tagaatcggc tgcccggaag
    15541 ccggcataca gcccgggcgg caggaacggc tgattcgctc tgcgatgagt acctcttcag
    15601 aaattaaagt aatggatcaa taaatattta accaaatact gtttatatca tgaaaaaaat
    15661 tgcactcttt cttttgacta tgatgatttg cggcattacc tctgcccagt tcgcaggtgg
    15721 catgttcggg cagcggcagg aaccgcttcc tgaaggttac aaaccggcct caaccaacac
    15781 ttttcttgca caatacccgg ctgtaaatgc tcagacacgc caggcaatct tcaaagtggt
    15841 agcaccacac gccgacaatg tggcgcttga cctcgccggg aaaaaataca gcatgaccaa
    15901 ggatgaaaaa ggagtatgga catgcacctc tgatccccag gtggtcgggt tccactatta
    15961 tgccatcctc atcgacagcg ttccggtcat ggacaggaac acgaattcgt actttggaag
    16021 caactggcaa tcatcaggga tagagattcc cgaaggaccc gaaggtgatt attacaggtt
    16081 caacaaggat atccctcacg gacacgtccg ttcgctttac tactggtcgg aaattaacgg
    16141 catggagcgc cactgcaatg tttatgttcc tgctgaatac gaaagtaacc ctgacaagaa
    16201 atatccggta atgtacctgc ttcacggctg gggtgaggat gaaaacggct ggtcaaacca
    16261 ggggcacatg gcaaatatta tggacggatt gattcatgcc ggcaaagctg ttccgatgat
    16321 agtggtgatg gactgcggtg acatcaaaac caactctgat gtccgcaaag cctctaccaa
    16381 cgatgtgacc cagatatata tcaaggacct gatgccgttc atcgagagaa ctttccgtac
    16441 aaagaatgac aggcagaacc gcgcaatggc cggtctgtcg agaggcggat tccagaccac
    16501 gatgactgtt tttgccaacc ttgacaagtt tgcatggatg ggaacattca gcggtttctt
    16561 ctctcgtccg ggtgataaca ttactgaggc tttcaacggc gtattcaagg atcctgcagc
    16621 cttcgacaaa cagatgaatc tcctgttcat cagtacaggc accgaagaac gcacaccgaa
    16681 agaggcagtt gacgccctga aggcacatgg aataaaaaac atcgtctatc acgaatcgca
    16741 gggtacagca cacgaatggc tgacctggcg ccgtgcactg aatgaatttg ctccattgtt
    16801 attcaaatag tgtgacaggt taataatctg ctttcgcttt atcgctgatt atcaacatta
    16861 acagatcggc aagcttttgc ctgcagacac ataatcaatt tgagcactca tcttaagaaa
    16921 gatgatcaag gctgataatc aggtaattat gagtaatcat ttttaaaata taccgaacta
    16981 ttgaacataa aaatgaaaac aaaactattg tttatagtta ttgtcactct ctttctgtca
    17041 ggcgccatgg tcattgcaca ggatcaggat ttccacatct acctctgctt tggccagtca
    17101 aacatggaag gagctgccag gattgagcca caggacacaa ccgttgacgg aaggttcatg
    17161 gtgatggaaa cccttgactg tccgaacctc ggaagagtca gggggaactg gtatccggcc
    17221 attccgcccc tttgcagatg ccgtaccggg ctgtcaccgg ccgattattt tggcaggacc
    17281 atgattgaac acctcccgga aaatatcagg gtgggggtta taaatgtctc aatcgggggc
    17341 tgcaaaatac aactgtttga caaggagaac tacaaggagt atgtctcaac tgcaccggga
    17401 tggatgatcc ctatgataaa ttcctacgac ggtaatccct atgcacgcct gatagagatg
    17461 gcaaaactgg cacaaaagga tggcgtgatc aagggaatac tgatgcatca gggcgagtcg
    17521 gataatggag acactacatg gcccggaaag gtccgcacag tctatgagaa cctgctgaag
    17581 gaccttgatc tgcaacctga aaatgtgcca tttcttgcag gtgaaatggt aagcgctgcc
    17641 gaaggaggga tatgtgcgga tatgaacgcc attatagcaa cactgccaca ggtaatacca
    17701 aactcctggg tgatatcatc ggagggttgc cccggaacac ccgatcatct tcattttacc
    17761 cctgaaggat atcgaatact cggaagaagg tacgctgcca ggatgctttc cctgctcgga
    17821 tacaacatcg atgtgccctg atcctgaatg atatagctta ttgagtgact gttccagtta
    17881 aaaacggcat ataacaatga ttatgaagag aaattgtttc atcctgatag tattaatcaa
    17941 tctccttcac tgtataacag tcaccggcca gaaagaccat gcccggaatc cggtaatctg
    18001 ggctgatgtt cccgacatgt caataatacg tgtcgggaac acatggtata tgaccagcac
    18061 caccatgcac atgtctcccg gcgtgcctgt catgaaatcc aatgacctgg tcaactggga
    18121 attagtcaat tatgcttaca atacgcttgg cgataatgat gcgctgaacc tcattgacgg
    18181 caggaatgcc tatgggagag gctcatgggc cagctgtatc aggtatcata atgagacctt
    18241 ctacgtatct accttttcct caacaaccaa taaaacatat atctggtcga cgaaggatat
    18301 agaaaaggga ccatggaaag aaatctcatt cacccccagt ttccatgatc ataccctggt
    18361 gtttgatgat gacggtcgtg tttacctcat atacggaaac aaaaagctaa cccttgtgga
    18421 gcttaacgct gacctgtcag gagtcaggcc cggaggcatc aaccaggtca tcattgaaga
    18481 tgcaagtgcc ccatcgggaa ctgacagcgg actgggagaa ggatcgcagt tgttcaggat
    18541 gaatggcagg tattaccttt tcaatataac ctggccacgt ggcgggatgc ggaccgtagt
    18601 tgtccacagg gccgacaggc tgaacgggcc gtgggaagga aaactcatct ttcaggatct
    18661 cggagtcgcc cagggaggaa tgaccgatac ccctgacgga agatggtacg catacctttt
    18721 cagggattac ggttctgtcg gacggatacc atatatcgtt ccaatgagat gggaggacgg
    18781 atggcctgtc atcggcattg acggcaaggt tcctgaagtg cttgatcttc ctccaggcaa
    18841 aggactcata cctggaatag tggcacctga tgaattcaaa agaaaaaagg gtgatccccc
    18901 tctcccgctt gtctggcaat ggaaccacaa tcctgacaat gccctctggt cagttactca
    18961 gagaaaagga tttctcaggc tgactgccgg gcgaactgat acccttttca cgcaggcaag
    19021 aaataccctg actcagcgca cactcggtcc tgaatgttcc gggtcaacac ttatagatgt
    19081 ttcgggtatg aaggagggtg attttgccgg gctggctctc ctacagaaaa agtacggtat
    19141 cgtaggggtt aaatatagca acgggatcag atcggttgtc atggtcaatg cacagtcagg
    19201 cagccctgaa gaggtggaga gcattccgct gacgcgaaaa aaggtttacc tgaaggcaga
    19261 atgtgatttc actgaccgga gggatatagc ttacttttat tacagccttg acggcaaaac
    19321 atggaaacca attggcaatc agcttaaaat ggagtattca atgcctcatt tcatgggata
    19381 ccggtacggg ctgttcaatt atgccacccg tgagccaggc ggattcgttg atttcgactg
    19441 gttccggata acaaatgaaa gaactgaagg tgaatgagaa acaactgaaa aacataacta
    19501 tcaaaactat gaagaactta agactatttg ctgcagtcct ggttttactg atcagcaccc
    19561 tcgccagttc gcaaaaccct gttattaggg accagttcac agctgatccg acagccaggg
    19621 tcttcaacgg gaaaatgtac cttttcccct cacatgacat ccctgctccg gaaagcttcc
    19681 cgcgaaaaga ctggttttgc atggcagatt accatgtctt ctcatcatca gaccttactg
    19741 actggacaga ccacggagtc attgtaagtc aggagaatgt cgcctgggtg aaccccacat
    19801 cgttcagcat gtgggcacca gactgcatag aaagaaacgg aaagtactat ttctacttcc
    19861 cggccaatgt gaagagcggc cgggggttcg gcataggggt tgctatagcg gataaaccag
    19921 agggacctta cactccactg cctgaaccga ttgcaaatgt aatgggtata gacccgagcg
    19981 tgtttatcga caaggatggt caggcatata tctattattc actcggcagg attttcgccg
    20041 ccaaactgaa agacaacatg actgaactcg catctgagcc ggttgttgtg ggtgatctcc
    20101 cgacaaaagg gcttgtggag gggccttttt tcttcgagag gaacggaatc tattatatga
    20161 catatcctca cgttgaaaat acaattgaaa gactcgaata tgcaattgga gacaatcccc
    20221 tcgggccttt cagggtgacg ggagttatca tggacgaaac cccgatgggg acctggacaa
    20281 atcaccactc cctggtgcag tacaatgacc agtggtatct cttctatcat aacagtgcct
    20341 actccccgaa ttttgacaag aacaggtctg tatgcattga cagcttattc ttcaatgacg
    20401 acggcaccat caggaaagtc attccaacca accgaggggt gggaataacc ggcgcgcaaa
    20461 ctacaatcga gatcgaccgc ttcagccagg taagcccgac aggtgtcaat gttgcactca
    20521 ttgataccac aaatactttc ctgggatggt ctgcagaact aaagggacaa aacagctgga
    20581 tctcatacaa tgcagtggat tttggcagga aaaagcttaa gtcagtccgt gtcaacgcct
    20641 catcgccggc aggaggaata atccatatcc ggcttgataa tcctgatggc ctgctcctcg
    20701 cggagataga aattccggaa agtacggccc tgagaaccgt ggatgcaaaa ctgaagaagt
    20761 acgaaaaagg gattcacaac ctttttgttg tactgaaaga tgaaaatccg gtcaacattg
    20821 actgggttca gttcaggaga tagtcaccgg gtgtctgatt acccaatgaa catgttaatg
    20881 gatcaaaaag aatcatgaaa tcgcttatta tcggctcttt cgcagcattg ccggttttgt
    20941 cagcatgttc ctccggcgtg aaaaccattc cggaggcaaa agatattata tggtatgaca
    21001 aaccggcagg ggaagcatga cttgtgttcc ggggcaaacg ggctgacagc catttccgaa
    21061 caacaggccc accggttttt gccgcataaa tgcgcttgac caaaagggct gacagctatc
    21121 tctgcactac aaccgttccc ctggtaatac ctgattcatt gaaggcatca acgctgaagt
    21181 agtatttcac atccctgttc agaccggtga gcatcaggga gccggtatca taaaccataa
    21241 cggaagtata tagcttcccg ggatcagtgc catagtttac aacatatccc gttgcctggt
    21301 cacaggcagg ccactccaca agggctttcc tggtatcagt ctcattgcgg ctgacagtga
    21361 aatccctgat ctcagcagga gcgtcaccct ccctggtacc gaagacgcgc aaatcataaa
    21421 gagagaattt tccatcaggc acactgacat tttcaatctt tatgaatctg gtcctgacag
    21481 gattctccag ctgaatgtaa tcatggcatg catcagtact gttgttgctc ttgtctgcaa
    21541 tgacatccca tgatttcccg tcgtgcgagg caagaattct gtaacggtag aagatatccc
    21601 tgctgtcagg tttaagtgtt gattcgtggt cagcaaaatt aacctgaatg gcggtcaccg
    21661 tggaggcatc atcgagttca acgctgagcc actccccgct gtcaccggta gcggctgacc
    21721 accatgtcct tatatcctca tcaaaagcat tgctgacagg gaaaccatcc acttccgatg
    21781 acgactctgc attcttctta tatgacagaa gcatccatcc tgtaaaagtg ttctcttttt
    21841 caaaatcaac cttccgttca ggcaaaattg ccggatagtc accgaaagca gtaaaggtcc
    21901 gcagtacacc ctcttcatcg aacgctgccg ggaacaatcc gagacgtctc tcgaacatat
    21961 gacgtattga tattgacatg gttgctatat gccagtaatt gccgtacttg tcctgaaatg
    22021 tggagccgtg tccggcgccg ccggcaaaac ctcccggttt ataggagaag gggctgtatg
    22081 attcatattc aaacggtccc agcggtgaag cggaggtata aacaccgtca gcgtaagtcc
    22141 tgtattccgt acccggggca gcatactgga ggtagtatct gttgttgtgc ctggtcaacc
    22201 atgccccttc attccatccg ttgctcgtca gctcattctg ctctcccggc acctcccagc
    22261 catagatgtc tgaattatgc tcaataagta tccggggttc agtgacagcc tgcattcctg
    22321 agttcagcat gactccatta atcggcaaaa agtttgagca tccccagtaa agatatctct
    22381 gtccgtcatc atcagcgaag aaatgcggat cccatactgc aaaaggaagt gtgtcattca
    22441 gtggtttcca gctatcactg aatggatccg tggtgtaata aaacggtttc ctttctgttg
    22501 acgacgcggc aaaccagacg gtatcattta tagctattgc atcaggggcg tagtcttcta
    22561 tcggcagcga gccgaccggc aggaaggtcc actcatgcag gtcatcagag taccagtacc
    22621 cgccgctgtg cgaaacaaac agaatatatt tgtctttgta ccggatcatg gccgggtctg
    22681 ctgcttcacg gtatgatacg ctgtctgtgt gctgaaaccg atagttgaga ttcagcggat
    22741 tgcagtaggt tctttcctgt ttaatcattt ctcctgaaca ggaagccagg ataagagaga
    22801 ttattatcag tgactttttc attgatatat gtattaagaa gtctatattt cgtgtggttt
    22861 ccggactttt catcaacaat aacgaaagat ataaaaactt gtagcaaaat cactttgcca
    22921 gacaataaaa gcaggccaca cgagcagttt ttaccactca taaattcgga gtgaaggatt
    22981 tttaaaacca atgacgtaaa aatgaaagga attatggctg tcacccccct ccctgaccgg
    23041 gatatttaat taaatttcaa actggttttg gatatcaaaa aaaataattg tcaaatgaaa
    23101 aagctgattg ttttgtttgg ttgtatggtt ctttccggaa ttctttccgg aaacctgatg
    23161 tctcagggca acgctgttat aaaaatcgat tttgaccgcc agacaggtaa agttgatccg
    23221 aacatatacg gagccttcgt tgaacctata aggaatgtgg tgtatggaag catttatgac
    23281 cctgcttctc ccttcgctga tgaaaatggt ttcaggaaag actttgtaga gcttgtacgt
    23341 gagctgaaaa ttccggttgt caggtggccc ggcggcaact atgtctcagg atataactgg
    23401 gaagatggca ttggcccgaa ggaagaacgg ccggtaagac ttgacctggc ctggaaccag
    23461 attgaatcaa atcatatggg cactgatgaa tatgtgcagc tctgcaaact tattggcgct
    23521 gagaatttca tatgcatcaa cgcgggaacg ggcaccctcg atcaggcaag gcactgggtg
    23581 gaatactgca acagggaaaa aggcacctgg tactctgacc tcagacggaa gtacggcaat
    23641 gaggagcctt ttaaggtaaa atactgggca ctgggaaatg agattgacgg cccctggcag
    23701 atggggcaga agagcgctga agactatgtc aagttcgccc ttgaggcagg aaaactgatg
    23761 caactggttg ataaggacat aaagttgatt gccagcggcg catcaaatta caaaccggat
    23821 aatgggtgga tagactggaa tgactacgtg ctgactcata tgacaggcac cattgattac
    23881 ctctcggtgc accggtatgc aaccgaggca ctgggtaatg acagaagctt ctcaggaatg
    23941 atgtgcctcg gacttgacct tgatcaaaaa atagagacgg tgaaggctct tatcctcaaa
    24001 gcgatgacga aaacaggatc aacacggccg gtctatatct ccttcgatga gtgggcagga
    24061 ggattcggga acaacatcac tgtatcacta atggtggcac aacatctcaa ttcatttata
    24121 cgtcatgccg atattgtgaa gatggctaat atgactatgc tttcaaacct tgtgggttat
    24181 tcgcctgaag gagtttacaa aaacgcaacc ttcatggcat tccggctctt cagcaataat
    24241 tgtttcggca cctctctcga tgtcagtacc acctgtgaaa aatacagtaa ttcaatcttt
    24301 aaagaaatcc cttaccttga tgttacagca gtgcttaacg acgaggccgg aaaccttgta
    24361 atcaacgtga tcaaccggca tgagacaaaa gccataatca cagatatagt tttgcagact
    24421 ggagaattca caggtaatgc aaaagtgaac gaggtgaacg gcaaaataac tgatccggga
    24481 aatccgggga cagcagaagg tgtcaacagt acatcatatg atatcaggtt caaagggaat
    24541 accattaaat attcattccc ggctcattca ttcacacaga tcattattcc gctcaagtga
    24601 aggttaaaca ctttcatcct atttgaatga tttttaaatc tctttgacta aaaatcaaaa
    24661 gtgtttataa gaactttttc tttgctttga atgcaaagca aaagtgttaa tactacactt
    24721 actgaaagcc gatttaattt tagagtccgc taacaaaacc tgactgccta tggaacaacc
    24781 cgaataaacc tcacctgaac cagtggattt ggaatatcca tgaaattaaa tcatagtaca
    24841 tacttctata tataaatgga aactcccaat taactaaaca accaagttat ggaacaaaaa
    24901 ctaaccaggc ctattcctct gttaaaacgg ggaatacaga ggcatttact gattgcaaaa
    24961 ttggtacttt ttacttttac cctcagtatg tttggaagca atgcatattg tctgggggca
    25021 gatgatctga atctgaattc agatattaca cttcaacaat taagggtcgc cggggttgta
    25081 agagataccg acggacagcc gattccgggt gttaccgtac aggttgaagg tacaacaaca
    25141 ggtgctttta cagatgtcaa cggaaggtac actgttgatg tacccgggcc caatgcagta
    25201 ctggccttct cttttgttgg ctatacacag gtgacggttc cggtaaatgg aagaaatgtc
    25261 attgatgtca ctctggaaga ggctacggag gcacttgatg agatagttgt tgtgggatat
    25321 ggcactgtca gaaagagtga tgttaccgga gctgtagcat ccgtcagggc agatgccatt
    25381 aaaaatgtat caaccactga tgctgctgct gctattcagg gtaaggctcc cggagt
//
LOCUS       DAOPBK010000008        25332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2107_length_25332_cov_5.134035, whole genome shotgun sequence.
ACCESSION   DAOPBK010000008 DAOPBK010000000
VERSION     DAOPBK010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2107_length_25332_cov_5.134035"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLA17_01100"
     CDS             complement(<1..860)
                     /locus_tag="PLA17_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84038.1"
                     /translation="MKKLTLLALVMLLAGVTGEVWSCTTFLMSGKHTPDGRPLLYKHR
                     DTGTLDNALVYFNDGKYDYIGLVDSKESWKRDVWGGFNAAGFAIMNSAAYNNNSGDTT
                     KLADREGEVMKMALATCATLADFEKMLNEMPKPVGVDANFGVIDAHGGAAYYETGNFS
                     WKKIDANDPALAPFGLIIRTNYSFTGDPDIGYGYIRYETASLALNMALAQKKLDPQNL
                     INCISRNLSHSLTKENFRNDLPESSADTRFRHIDDFITRTSTASAMLVVGALPGEDPA
                     STMMWTLVGFP"
     assembly_gap    1133..1232
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1317..2990
                     /locus_tag="PLA17_01105"
     CDS             1317..2990
                     /locus_tag="PLA17_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84039.1"
                     /translation="MKKFFCTTVLTLLIASAAIAGRQDYPEEYLGLPGDNLNLYAVMN
                     LFQESETLEAFERSLNEENTRINNLDLNNDGYVDYLTVSDYVNGNVHTIVIRSVLDRN
                     EFQDVAVFTVEQLRNGEVQIQLIGDEALYGRNYIVEPNYAETPNPGYKGRTVQRKNVT
                     VVTTTYYEVAAWPVIRFIYSPRYVVWRSSWYWGYYPVYWRPWKPFFWHYYYGYHYHWY
                     PHYYTYYRHWHQPRWIHYRDYYYGSVRVYSPNVSHRITAGTYKQTYSRPESRRDGEAL
                     YAKTVETRSRSVSSQQAEAGRRQSATTAATQSQSRTRSTETSAGSDRRSPATAGSRPV
                     SSETRRSATTQPSTRTQTSNEGRSSAPATRTQTSSSGRSNAPAAGSERRSPATTGNSS
                     VSSDTRRSATTQPSTRTQTSNEGRSSAPATRTQTSSSGRSNAPAAGSERRSPATTGNS
                     SVSSDTRRSATTQPSTRTQTSNEGRSSAPATRTQSGSSGRSSAPAARTQSGSSGRSSA
                     PAARTQSRSSGRSSGTVSSRSSNSRSSGASSKSSSSSGNSDNSSRSSRR"
     gene            complement(3146..3796)
                     /locus_tag="PLA17_01110"
     CDS             complement(3146..3796)
                     /locus_tag="PLA17_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPK84040.1"
                     /translation="MGQKRARMVTVTGTVTDTTMSPVHGVLIIADGQSTGVVTGGNGT
                     YRIRIRPDVKSLGVYSTNMGSALTEYEGSPVVDFVLDGRDVLRNFKFEMPEGEKEIEV
                     AYGTVKKKNLTTDVGYIDGQAHVNSSYTNIYEMIKGKVPGVVVSGNKITIRGTNSILL
                     SSDPLFVVDGSVVNSIDHINPRDVKSISVLKGSDAAIYGSRGAGGVILITLVGTKR"
     gene            4021..5223
                     /locus_tag="PLA17_01115"
     CDS             4021..5223
                     /locus_tag="PLA17_01115"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK84041.1"
                     /translation="MTFDELDLDPGILDAIGYMGFREATPIQEKAIPVILEGHDLIAC
                     AQTGTGKTAAYLLPVMNDIAYHRPSHTHTLVLVPTRELALQIDQQIQGLAYTQNITSL
                     AVYGGGEGSAWDQEKAALTHGADIIVATPGRLISHLNLGYVQFTELQVLVLDEADRML
                     DIGFHDDIMKIISFIPKKRQTLMFSATMPPKIRSLSKHIMKNPKEISLEMSKPADGVL
                     QAVYLLEEPQKLPLLNSLLADKPEYHSILIFSSTKKKVHEIVRGLRSRDYTVEGISSD
                     LEQKEREETLLRFRSRRTRVLVATDVLSRGIDIKEINLVVNFDAPSDAEDYVHRVGRT
                     ARADATGVAITLVTRDDMFKLRRIEKLIGYQIFRAPLPDFLPSGQSDTAGRRHSRPRS
                     GNRKFRRR"
     gene            5456..>6803
                     /locus_tag="PLA17_01120"
     CDS             5456..>6803
                     /locus_tag="PLA17_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84042.1"
                     /translation="MKKSVITSVLAVLTALTTIVGAQNYPKEYLGLPGDNLNLYAVLD
                     LFRNAPTLEAFERSLNEQDSRINNLDLNGDWRVDYIAVSDYRDGRVHNIVLRAMLGRN
                     EYQDVAVIVVKRLKNKKRVLIQIIGDELLYGRNYIIEPVYVKKHRIRHEYSPVYYGST
                     TIYNNYYYDIWEWPVVVYIYSPYYDGWSSSWYWGYYPAWYEPWSPWYWDYYYGYHSGW
                     HSHYYSYYHHSDRYRYNRYNDYYYHSVRQESPQVQRRVAEGNYRQTYSRPEQRAEGMD
                     LQRRTREAIAEGTRSQAPANAGTRRQTPPQAVSGTVPSEGRRTAAGTEGRRTAETGAA
                     GRTSTGTVSRSAGENGQVRRTGTGTGSREAAATKATRRSASGTVRRETAPTEEARRAP
                     ATVSRTVQNGERNSEVVETGRRSAASAPATVKNTESKSAEARENERRAAEAREAQNR"
     assembly_gap    6804..6903
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6904..7175
                     /locus_tag="PLA17_01125"
     CDS             <6904..7175
                     /locus_tag="PLA17_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84043.1"
                     /translation="RENERRAAEARENERRAAEAREAQNRENERRAAEAREAQQRAAE
                     QRAAQARENERRAAEARENERRAAEAREAQARENERRTAGSRAAR"
     gene            7506..7973
                     /locus_tag="PLA17_01130"
     CDS             7506..7973
                     /locus_tag="PLA17_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84044.1"
                     /translation="MKKLLFIVVLISLSAVAGAQISSNALGARLYGGDSFSGAELSYQ
                     KGLNKRNRLELDASFGFANEFTRVALVGIYHWDWNIVGGFNWYVGPGASIAYDNYNDN
                     SYINIGLGGQIGIEYKFKDLPVLVSLDTRPMWDFLGEVNGLGWGAALGIRFTW"
     gene            8012..8506
                     /locus_tag="PLA17_01135"
     CDS             8012..8506
                     /locus_tag="PLA17_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPK84045.1"
                     /translation="MKYLLNILLLLLPLVMSGQAASSEPEVWFTVTRVVDGDTFWVDD
                     GSEKGMKIRLIGIDAPEPRNTGTRPKGFFGAESTSYLQNLLKGKKVRLEYDVARYDRY
                     RRTLAYAFLEDGTFINAELVRNGYATVMTMPPNVKYAETFNKLASKARKQKKGLWKES
                     PFVK"
     gene            8537..9481
                     /locus_tag="PLA17_01140"
     CDS             8537..9481
                     /locus_tag="PLA17_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84046.1"
                     /translation="MKKIALLIAFAAIATTSLCQVDTTSVSYVAYWSIGDSYDFRVHK
                     TSMQWSGDSLIKNDISEYIANFEVVDSTSTSYTVVWTFSNELANTWDIPDEMLEELSK
                     YSLTEVVYTTTELGEFTSIENWKELGEMMRSMFSDIFRVLTKDSGEEYDKLMQTARPI
                     IDMYSSKEGVEQIVFPELMLFHFPFGAMIPVADTISYEDLLPNLLGGVPIRADAKLWF
                     EEVDFEEERSVLVQESKLNTDDTMDLLRSLFDRMGLKDASLTEALETAVYEINTSARY
                     EYYYYPGVPINIESKKNMTLDVAGERARRDETITIELL"
     gene            9523..10158
                     /locus_tag="PLA17_01145"
     CDS             9523..10158
                     /locus_tag="PLA17_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPK84047.1"
                     /translation="MPLKELQPIFIFLAVVLLLLSASCSRRTVPADAVHGKSGRSAGG
                     VLIKTSEFTLIIPDVSLSQSETKQLEQFINAGIGLSVASNKATPEDIIRTARGYLGVP
                     HCMGGTTAKCMDCSGLIFRIFARHGITLPHNSEEQARYGRIITEKKMLMPGDLVFFVR
                     TYSSSRLITHSGIFIGEDRFIHTSSGKGVTITPLNDPYWKERYLFGTRIFE"
     assembly_gap    10380..10479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10567..11556
                     /locus_tag="PLA17_01150"
     CDS             10567..11556
                     /locus_tag="PLA17_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPK84048.1"
                     /translation="MSQGTKQPDDANYGGDYSYAIKSALFGVAVGDALGVPVKFKSRE
                     YLNMHPVTGMTGFGSHSQPPGTWSDDSSLTFCLAEALIRGYDLDAIGQNFVKWYSNAY
                     WTAGGEVFDIGKGTDEAIVRLSGGTKPELSGDTSVSSNGNDSLMRILPLVFRLMPKPL
                     TERFEITRQVSAVTNGHIRSIISCFYYLEFARQILEGREKYDIYRDIRKVVSGHLSAL
                     LIDPAEVLVFDRLLKYDIFRLNESEISTGSNVINTLEAAVWCLLTTESYSEAVLRAVN
                     LGGDTDTTAAVTGGLAGLLYGFDGIPEEWIRQIRRKEDIEELAEKLAAGLNGN"
     gene            11781..12302
                     /locus_tag="PLA17_01155"
     CDS             11781..12302
                     /locus_tag="PLA17_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84049.1"
                     /translation="MKSRILTAMITALVTIILIAVAVYFIFGFNSKPGKVMSVNSPQG
                     SFEAYVIESPSADPPKQSLFIAKAGTGEFRLVASLPEDIESTQTIYWTEEGDKAIFVT
                     NWHLFVTDVQTFNTMKISLNPDWWKMHEGEKTFSSSGTPVVMEELILDGSDSLTYRTN
                     LMTQPVTVSLTGL"
     gene            12318..12734
                     /locus_tag="PLA17_01160"
     CDS             12318..12734
                     /locus_tag="PLA17_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84050.1"
                     /translation="MEQRDYLLREIEKIGAIIMVIRRKLFGGTDEPAITVENQAEALK
                     EMLLSEAFIDLNELLAMDAEDTDKYLAGHEGFNVANIELLAQTLSDLGMTSTPPFSFA
                     LLEKALQLYKICSLRDRTYSFEREAAISTIREALKA"
     gene            complement(12820..13161)
                     /locus_tag="PLA17_01165"
     CDS             complement(12820..13161)
                     /locus_tag="PLA17_01165"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HPK84051.1"
                     /translation="MKGIRIALALILLAISAASPAQKVFSVKYENQADVKVYVVKYEN
                     QADLKVFKVKYENQAGENNGRWFFTKYENQAKKKIYFVEYENQSDLKIFFVDYENQAG
                     WRNKEKIHLMY"
     gene            complement(13170..13400)
                     /locus_tag="PLA17_01170"
     CDS             complement(13170..13400)
                     /locus_tag="PLA17_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84052.1"
                     /translation="MKKLNYVSGILTGIAAAVAVFLLVSHKPASEPAVPQYSGKITSI
                     NSTTDVYRLSVDNAQYIVVVSHKGGVAVTRHK"
     gene            complement(13673..14329)
                     /locus_tag="PLA17_01175"
     CDS             complement(13673..14329)
                     /locus_tag="PLA17_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84053.1"
                     /translation="MPANLNALIRYKTINSCLSGGRRRWSIDELRERCSEALASDRGR
                     YTTVSERTIRDDIRVMRSDILGFNAPIKQEGGLYFYSDPGYSILTLRITDSGLAEQIL
                     GLLLKIRGKVNHPEMEIVIEKLSTLTGRKYELESELTDRDDFYEEGTKVMNQTIWEES
                     QADELLSDDLEDKYPVEHGTRLRFSKDRYYSCRSFMQPPELKWGEIMAVMSGVSRRQT
                     "
     gene            14500..15420
                     /locus_tag="PLA17_01180"
     CDS             14500..15420
                     /locus_tag="PLA17_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84054.1"
                     /translation="MMKTKAIHFTAFGNAAIVQVSSQPLSSFPFLPAGPAIRNGGLVI
                     SELTASGSVGRLAALNKTAGWLLLTDADILTGAKQNRVVNRSVLLAPSSKTVIEVSCV
                     ERLRWDYRSTSFSTPGSVADPNLRSKKAGTFSYMKHEPDFRNTQDEVWDHVSMNLACD
                     RVYSKTENYQELLQSRMEKRHHDLPECSPAEGCNGLAVILDGKVISADIFGNEESYRH
                     YFPMLRDAAFTLSRHGADCEQPEMHEAYFRALEAIDEFSDAKRHPEEKHPGAGTLSMV
                     ETERLVGFSLMHGKELIHNALFARHDQATK"
     gene            complement(15444..16934)
                     /locus_tag="PLA17_01185"
     CDS             complement(15444..16934)
                     /locus_tag="PLA17_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPK84055.1"
                     /translation="MEIQLELILLGLSVLFLVSIYAGKASSRFGVPALLLFLSVGMLS
                     GSDILGIEFGDIHIAQTIGTVALCIILFSGGMDTRMSEIRPVIPQGAILATIGVMMTA
                     FLTGLAIWVLLGMTPGASGIGLLTALLLASTMSSTDSASVFSILRSKGLHLKNNLRPL
                     LELESGSNDPMAYVLVVTFIDIINMGSSPNYWLAAGTLVLQLIVGTASGYLVGKLAVY
                     IINHIKIENTSLYPILVFTFCMMIFSLTYFLKGNGFLAVYIGGLVIGNSKLVHKRSSL
                     NFFDGLAWMSQLIMFLTLGLLVNPSELIPLLIPGLIISFIMIFVTRPLTVFLCLAPFR
                     KMPVRDKVFVSWVGLRGAVPIVFAILPLAADVPHARLMFNIVFLCTLVSLLVQGTTLP
                     KVAKLLGLAETPVEAKKLEEFDVEFSGDIKTIISELVVTPRALENGKNLMELPLPENT
                     LVVMVKRDDAYFVPTAKTPLHVGDNLLIITESSETLEETYRVLGVE"
     gene            complement(17044..17541)
                     /locus_tag="PLA17_01190"
     CDS             complement(17044..17541)
                     /locus_tag="PLA17_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84056.1"
                     /translation="MGIIGYVIVFGLVLLILSPIIAGHFKNVRKDKLLLATLKDSGSA
                     KGLSLTMTDAWKGAYAIGIDPEKRRLIYLRKAGESIQEELEDLSALSDCRVDITSRTE
                     KTPNGSMTVVEAVNLIIRYSDKTLSDKKLEFFNNKVFHSLAGERTLAEKWQKKISSIL
                     QTKNR"
     gene            complement(17680..18054)
                     /locus_tag="PLA17_01195"
     CDS             complement(17680..18054)
                     /locus_tag="PLA17_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84057.1"
                     /translation="MIRQRLFKGILLGLTFLFLCHGIRGYYPRYNEPLQEDIPFSRTV
                     MDVGNYPVVLILNEGNSNPADVLKQPPAPCDRPLAQGMSDLPVWFGACEAVKESAFRD
                     YAGTVYIDVSTTDLIFPFHYFL"
     gene            complement(18300..19052)
                     /locus_tag="PLA17_01200"
     CDS             complement(18300..19052)
                     /locus_tag="PLA17_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HPK84058.1"
                     /translation="MYTRGIVRRPGRNFAGGITTAKLGKPDFGKALEQHAAYCEALIK
                     CGVKLTVLEADDRYPDGCFVEDTAVVNNRVKVVSRPGAPTRLGEEEAIIQVLAGYGPT
                     EAIIAPGTMEGGDILRAENHYFIGISERTNTWGANQLSAILAKHGFTTSVIRVEAGLH
                     LKSDIAYLGDGNFISTPVFSHVAHPANTIILDPDEYYAANCLRVNDYLLIAKGFPKLK
                     KKILELGYNIIELDMSEFRKMDGGLTCLSLLF"
     assembly_gap    19328..19427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19928..20506
                     /locus_tag="PLA17_01205"
     CDS             19928..20506
                     /locus_tag="PLA17_01205"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HPK84059.1"
                     /translation="MDKWIYQLRITLIDSRPRIWRRILVEEDVLLSDLHKIIQTTMGW
                     TNSHLHQFIKDETFYTKRMPDDDYWEEMNNVDYKGIKLNEILRFVKNTIIYEYDFGDG
                     WEHEILLEKFLKPDSNVKYPVCIDGAMKCPPEDVGGMGGYMDMLKVLRNPNHREYESF
                     INWLGEEFDPKEFDISMVNKLLAKRNYGLPRF"
     gene            20692..21006
                     /locus_tag="PLA17_01210"
     CDS             20692..21006
                     /locus_tag="PLA17_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HPK84060.1"
                     /translation="MNCGLGETDPATGPGNNETVMPFISSASIACGFQADDLLTLYRT
                     MPPAIRHGVSIVAHPWYPDCQTSGGYARIANVITVDLTLLAQMRPGDKILFKETLLDF
                     VQ"
     gene            complement(21105..23279)
                     /locus_tag="PLA17_01215"
     CDS             complement(21105..23279)
                     /locus_tag="PLA17_01215"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPK84061.1"
                     /translation="MLEFIPTIVYKRIFMLSLFYSLAILSSAQPQIDAVRVDNPPHID
                     GRVDDDVWNLAFKVYEFFQQEPNFGEPSSEKTEVFVCYDSKNIYFGIKCWDNPDKIAA
                     KEMLYDARISNDDRMRILIDTYHNKRTGYYIAINPLGAKIDASLSNDGSLKNEDWDGI
                     WDGRAHITDWGWEAEIAIPFQTLGFNRNNDTWGIKFSREIEYKAETSYWPKVGINSTS
                     SQLSDAGILVGLIDISQGLGLSFLPYIVTGFDQKRNNGSDYRFSAGLDISYHITPSLK
                     TAISLNTDFAETEVDNRQINLTRFSLHYPDKRNFFLDGANYFQFGNETGGAKSVQDKV
                     ITFFSRRMGLDTLGQPVPINYAVKFTGMAGGWNIGALYINDDREKEKNNFYVARVNRN
                     FLKQSSVGFIGTYGNTLGPESNFVVGLDLKLATSTFRGDKNAALILFGQKSFTKEVAN
                     TNNSSYGIQVDYPNDFIKANLGYYRVEKDFIAGMGFVPRTDINEIYGRLTLGPRLKKY
                     GILQISSGGEFSIIKNASTNDLETREIKLQPVGIRFLSGDQLTYSLVSQYEYLKEDFN
                     IFDDVVITTGGYSWWQNEIKLETKGARDIWGEITYGFGDFYDGKQKEIEIAANWKISV
                     PFLLACKLKKNIIDLPEYSFTSNVYQFNANILFSPNLTLYNFVQYDNASNTIGLQSRF
                     RWILRPGNEIFLVWNSKYVEADSGYISDTGALRFKMKYSIRF"
     gene            complement(23618..24736)
                     /locus_tag="PLA17_01220"
     CDS             complement(23618..24736)
                     /locus_tag="PLA17_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84062.1"
                     /translation="MKLIKTIAVLVLFSFVLDAQESGTIFSFTEGGLCGEVQVKPWDV
                     LDDVSVTFRLYASRTNEVLVNVNWPEGRCIAMIKSCNNVWKDTTRDNQSELWTYTFSV
                     DGIRTIDASNYNISRYGVGFMNTVLVPRDMLEFMQAYQMSHGIRSTIWTPSTFIKVSS
                     HAFIYRLLRYEGCEASCPVLYLLHGRGGDEDAWPAMGIASIIMDQLIVQAKASPMIAV
                     MPNTYWNELASFDLAGPCFAPPLGMGGSGNRVISGASRALGTVAPTTPGGIGIVPPST
                     PGGEGNTQRDYSKNVQNIVGRLIPVIEKNFQTLHGSKNRAIAGLSMGSQITANAERSR
                     LDVFWSMGLLSSGMFRSLPGRIVEIEAIPRTFLKILYK"
     gene            complement(24798..>25332)
                     /locus_tag="PLA17_01225"
     CDS             complement(24798..>25332)
                     /locus_tag="PLA17_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84063.1"
                     /translation="NPLWLEFDRNDIPPFKKYEIVGVPDGYVFNLGGDYDVELIFTGG
                     HTNEHVMFLDKKGRFLFAGDNVCSDVTGCGNGGGKYANLSTFSKALTKLVERINEYDY
                     IFPGHFMVNLENHLMFSLLETVNAVINDPNCYDYKITHISGGGGPGRVRMHKFIRGFS
                     TIAYTESGVYVPDEPTI"
BASE COUNT         6707 a         6199 c         5881 g         6145 t
ORIGIN      
        1 gggaagccga caagtgtcca catcatggta ctggccggat cttcaccggg gagagcaccg
       61 acaaccagca ttgccgatgc tgtcgaagtc cgtgtgatga agtcatcgat atgcctgaac
      121 ctggtatctg cggagctttc aggaagatca ttgcgaaagt tttcctttgt cagggagtgt
      181 gacaggttgc gtgatatgca gttgatcagg ttctgcggat cgagcttttt ctgtgccagc
      241 gccatgttca gggcgaggct ggctgtctca taccttatat aaccgtagcc tatgtcagga
      301 tcaccggtga aggagtagtt ggtgcgtata ataagcccaa acggggcaag tgccgggtca
      361 ttggcatcaa tctttttcca gctgaagttg ccggtttcat aataggctgc acctccatgg
      421 gcatcaatga ctccgaagtt ggcatcaaca ccaacgggtt ttggcatttc gttgagcatc
      481 ttctcgaaat cagccagggt ggcacaggtg gcaagtgcca tcttcatcac ttcgccctcg
      541 cggtcagcca gtttcgtggt atcaccgcta ttgttgttat aggctgctga gttcatgatg
      601 gcaaagccgg ctgcgttaaa tccgccccat acatcgcgct tccacgactc ctttgaatca
      661 accaggccga tatagtcata cttaccatca ttaaagtata cgagagcatt gtcaagggtg
      721 ccggtatccc tgtgtttgta cagcagcggc ctgccgtcag gggtgtgctt gccagacatc
      781 agaaatgtgg tgcatgacca aacctcgcca gtgacgccgg ccagcagcat cacgagcgct
      841 aacagtgtaa gtttcttcat agggattaaa atatagtttg gataaagtta tgcttttttt
      901 aacccgtttg agtccggcag gtaacatgtt aaggtttttt aaccctgcgg tctgtctgca
      961 aaacggcggg ccgggagtca ctgcaactga tgatgatggc agcatgccag acagggtcag
     1021 tctgatgata gtctgaatgg gacattgaat tcaaagcata cctggcaggg tcagtccgat
     1081 gatagtctga atggagcata gcagaacaaa gaatgcctga gggcaggcaa tgnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncttatagc tattttgcgc aatttttggc
     1261 ataacttttg aattttacaa ttgcgaagag acagttcttc ataaaaacgt aaagagatga
     1321 aaaagttttt ctgtactacg gtcctcacac tcctgatagc ttcagcggcg atagccggaa
     1381 gacaggatta tcctgaggag tacctgggtc tcccgggtga caacctgaac ctctacgcag
     1441 tcatgaatct ttttcaggag tcagagactc ttgaggcctt tgaaagaagc cttaacgaag
     1501 agaacaccag gatcaacaac cttgacctga acaacgatgg ttatgtagat tatctgactg
     1561 tgtccgatta tgtcaacggc aatgtccaca caatagtaat ccgttcggtg cttgaccgca
     1621 acgagttcca ggatgtggca gtattcaccg ttgagcagct tcgtaacggc gaggtccaga
     1681 tacaacttat cggtgacgaa gctctttacg gaaggaatta cattgttgaa ccaaactatg
     1741 ctgagacgcc taatcccggt tacaaaggac ggaccgtcca gaggaagaat gtgaccgttg
     1801 tcaccaccac ctactatgag gtagcagcct ggcctgtcat caggtttatc tacagtccac
     1861 gctacgtcgt atggcgctcc agttggtact ggggttacta ccctgtctac tggcgtccct
     1921 ggaagccgtt cttctggcac tactactacg gttaccacta ccactggtat cctcactact
     1981 atacttacta ccgtcactgg caccagccga ggtggattca ctaccgcgac tactactacg
     2041 gcagcgtaag ggtttattca cccaatgtat cgcaccgtat cacagccggt acttacaagc
     2101 agacctactc taggcctgaa tcaaggagag acggtgaagc gctttacgca aagacggtgg
     2161 agacacgcag caggtcagtc agcagccagc aggcagaagc tggcaggaga cagtctgcga
     2221 ctacagctgc aacgcagagt cagtcccgca cacgcagtac cgaaacgtca gccggcagtg
     2281 acaggagaag tccggcaact gcaggcagca ggccggtcag tagtgaaaca cgcaggtcgg
     2341 caacaaccca accctcaaca aggactcaga ccagcaacga ggggcgcagc agtgctccgg
     2401 caactaggac ccagaccagc agcagcggac gcagcaatgc accggcggcc ggaagtgaga
     2461 ggagaagccc ggcaaccact ggtaacagtt cggtcagcag tgatacacgc aggtcggcaa
     2521 caacccaacc ctcaacaagg actcagacca gcaacgaggg gcgcagcagt gctccggcaa
     2581 ctaggaccca gaccagcagc agcggacgca gcaatgcacc ggcggccgga agtgagagga
     2641 gaagcccggc aaccactggt aacagttcgg tcagcagtga tacacgcagg tcggcaacaa
     2701 cccaaccctc aacaaggact cagaccagca acgaagggcg cagcagcgca ccggcaacaa
     2761 ggactcagtc cggcagcagc gggcgcagca gtgcaccggc agcaagaact cagtccggca
     2821 gcagcgggcg cagcagtgca ccggcagcaa gaactcagtc cagaagcagc ggacgcagca
     2881 gcggaactgt ctcaagccgt agcagtaaca gcagaagcag cggggcctct tcaaagagca
     2941 gcagttcatc agggaattca gataacagca gccgctcatc gagaagataa ggggcacaga
     3001 gcaggcttgc tcagtgcatt gattatatct ccaggtgcag ggaaaaacaa ccctgcacct
     3061 tttatttcac tattatccgg ttaataacag gcgatttgca gtccgctttc agtccattgt
     3121 catcccgggg agacggcggc atcctctacc tttttgtgcc gacaagggtt atcaggatga
     3181 ccccgccagc acccctggaa ccgtaaattg ccgcgtcaga gcccttaagc acacttatcg
     3241 acttgacatc gcgggggttg atatgatcta ttgaattgac cacagagccg tcaaccacga
     3301 agagagggtc actgctgagc agtatggagt ttgttcccct tatggttatc ttgttccctg
     3361 agaccacaac cccgggaact ttccccttta tcatctcata gatattggta tagcttgagt
     3421 taacatgagc ctgtccgtca atgtaaccca catcggtagt cagattcttc ttcttgactg
     3481 taccgtatgc aacctcgatt tccttttctc cttcgggcat ttcaaactta aaattcctca
     3541 gcacgtccct gccatcaaga acaaaatcaa ccaccggtga tccttcatat tccgtcaatg
     3601 cggagcccat attcgtgctg tataccccga ggctcttcac atccggcctg atccttatcc
     3661 tgtaggtgcc atttcctcct gtcactaccc cggtactctg cccgtctgct atgatcagca
     3721 ctccgtgtac aggagacata gttgtatcgg taactgttcc tgtgaccgtc accattctgg
     3781 ctcgtttctg tcccatggcc tgccaggatg caacagtcag aatcaatatc attaaaattg
     3841 tctttgctct catgaaatat ggtttaatat tacgcaagtt acgaaaatga gaatcatttt
     3901 tcaaaattat cctgtgacac cggggctcat ctccggccac taattctgcc tgccaaagtt
     3961 ttcgctcttt ttttactact tttgctgccc tccacactgg agattattaa tcaaatgttt
     4021 atgacttttg acgagcttga tctggaccct ggaattcttg atgcaattgg ctatatggga
     4081 ttcagagagg ccaccccgat acaggagaag gcgattccgg taatccttga agggcatgat
     4141 cttattgcct gtgcacagac aggtaccgga aagacggctg cctatctcct gcctgtgatg
     4201 aatgacattg catatcaccg tccttcacat acacacaccc ttgtacttgt tccgacgcgt
     4261 gaactggcgc ttcagattga ccagcagata caggggctgg catacaccca gaacatcacc
     4321 tcactggccg tttacggagg aggtgaaggg agcgcatggg atcaggagaa agctgcactg
     4381 acacacgggg ctgacataat cgtggcaact ccgggaaggc ttatatcgca tcttaacctc
     4441 ggctatgtgc aattcacaga gttgcaggtg cttgtgcttg acgaagcaga caggatgctt
     4501 gacataggtt tccatgatga catcatgaag atcatctcct tcattccgaa aaagcgtcag
     4561 acactaatgt tcagtgccac aatgccacca aaaatcagat cgctttccaa acatataatg
     4621 aagaacccga aggagatctc cctggaaatg tcgaagccgg ccgacggggt tctccaggct
     4681 gtttacctgc ttgaggagcc gcagaagctc cccctgctca acagcctcct ggcggataag
     4741 cctgaatacc acagtatcct catcttcagc tcaacaaaga agaaggtgca tgagattgtc
     4801 aggggacttc gctcacgcga ctatacggtg gaaggcatct catcagacct ggaacagaag
     4861 gaacgcgagg agactctgct gcggttccgc tccagacgga cacgtgtact ggttgccacc
     4921 gatgtgctga gccggggtat cgatataaag gagatcaacc tggtggtcaa tttcgacgca
     4981 ccctctgacg cggaggacta tgtgcaccgt gtaggccgta ctgcccgcgc tgatgccaca
     5041 ggggtggcaa taaccctggt aaccagggat gacatgttta agctccgtag gattgaaaaa
     5101 cttatcggtt accagatatt ccgtgcacca ctgcctgact tccttccttc aggtcaaagt
     5161 gacactgcgg gccgccgtca ctcgcgtccc cgctccggga acaggaaatt cagacgccgc
     5221 tgacggtaaa atcctgcatg tgggcactta tttatcctga ccgcccggag acaaatctga
     5281 agctgatcag ggaaggaata tgacccggtg actataagcc atataccact cctgaagata
     5341 tcatacatat tacaaaaaac aatctgacag cctcatctca tgagggtttg cgaagatttt
     5401 ggcacaccct ttgcattacc ttcagtgaag agtgatcttc ataaaactta aagagatgaa
     5461 aaagtcagtt atcacatcgg tcctcgcagt tctgacagcc ttaacgacaa tagtcggggc
     5521 acagaactac ccgaaggaat acctgggtct ccccggtgac aacctcaacc tctacgcggt
     5581 tctggacctg tttcgtaacg ctcccaccct tgaagctttc gagagaagcc tcaatgagca
     5641 ggactcacgg atcaacaacc ttgaccttaa cggcgactgg agggttgact acattgccgt
     5701 atcggactac cgcgacggca gggtgcacaa catcgtgctc cgcgcaatgc ttggacgcaa
     5761 tgagtaccag gatgtggcgg tgatagtagt gaaaaggctg aaaaacaaga agagggtttt
     5821 gatacagatc atcggcgacg agcttctcta cggccggaac tacataatag aaccggtcta
     5881 cgtcaaaaag cacaggatca ggcacgagta cagtccggtc tactacggca gcacgacaat
     5941 ttacaacaac tactactatg acatatggga atggccggtg gttgtctaca tatacagtcc
     6001 ttactacgac ggctggagct catcatggta ctggggctac taccccgcct ggtatgaacc
     6061 ctggagccca tggtactggg actactacta cggataccac tccggatggc acagccacta
     6121 ctacagctac tatcaccaca gtgaccgata caggtacaac aggtacaatg attactacta
     6181 ccacagtgta aggcaggagt cgccccaggt acagcgcagg gttgccgagg gcaactacag
     6241 gcagacctac tcacgccccg agcagagggc tgaagggatg gatctgcagc gcagaacccg
     6301 cgaagcgata gctgaaggta cccgcagcca ggctccggcc aacgccggca ccagacgcca
     6361 gacaccgcct caggctgtga gcggaacggt tccctccgaa ggcaggagaa ccgccgcagg
     6421 caccgaagga cggcgtacag cggagactgg agcagccggg aggacttcca ccgggactgt
     6481 cagcaggagt gctggggaaa acggacaggt aagaagaacc ggcaccggga ccggcagcag
     6541 ggaagctgcc gcaaccaaag ctaccaggag gagtgcctcc ggcacggtga gaagggaaac
     6601 agcaccaact gaagaagcaa gaagggctcc cgcaacggta agcaggaccg ttcagaacgg
     6661 cgagcgcaac tcagaggtgg ttgagacggg cagaaggagt gccgcctctg cgccggcaac
     6721 agtgaagaac actgaaagta agagtgcaga agcacgcgag aatgaaagga gagcagccga
     6781 ggcacgcgaa gctcagaacc gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnncaaggga gaatgaaaga agggctgccg aagcacgcga gaatgaaagg agagcagccg
     6961 aggcacgcga agctcagaac cgcgagaatg aaagaagggc tgcagaggcc agggaagcac
     7021 agcaaagagc agctgaacag agagcagcac aagcaaggga gaatgaaaga agggctgccg
     7081 aagcacgcga gaatgaaagg agagcagctg aggcacgcga agctcaggca cgtgaaaatg
     7141 agaggagaac tgccggaagc agggccgccc ggtaaagaac aatggtatcg gattccaaca
     7201 caaccatcat cagcaaataa cacataactg actgttcata gtcccgctga ccaccagatc
     7261 agcgggactt ttcattccac tccggaatta gtaaaggata ttggtctcca agagctgaaa
     7321 gtccgttctg cagactttca cgctccatca cacatatctg ctttggaatg aattttgttt
     7381 aactggttat gccattcttt cagacaggca ggttattttt gccattagag gctattaata
     7441 ttacgaaaaa tgtaaggcgg aaccgaaaag ccatacgagt aggaaaacgt taaaaccatt
     7501 ttatcatgaa gaaactgttg tttatagttg tactaataag ccttagcgct gttgcaggcg
     7561 cacagatcag ttcaaatgcc cttggtgccc gcctttacgg tggagacagc ttcagcgggg
     7621 cagaactgtc ataccagaag ggactcaaca agagaaacag gcttgaactt gatgccagct
     7681 ttggtttcgc taatgaattt accagggtag cgcttgtagg aatctatcac tgggactgga
     7741 atatcgtcgg aggattcaac tggtatgtag gtccgggtgc aagtattgca tatgacaact
     7801 acaatgataa ttcctacatc aatataggcc ttggcggtca gattggcatt gaatacaaat
     7861 tcaaggatct tcctgttctg gtcagccttg acacccgccc gatgtgggat ttcctgggtg
     7921 aagtcaacgg gctgggatgg ggtgcagccc taggtataag gtttacctgg taacacagca
     7981 caccgggaaa acccggtttt ttaacaagaa agtgaaatac cttctcaaca tactgctgtt
     8041 gctcctgcca ttggtcatgt caggtcaggc agcatcatcg gaaccggaag tgtggttcac
     8101 cgtcacccgc gtggtagacg gtgacacctt ctgggttgat gacggcagcg agaagggaat
     8161 gaaaataagg cttataggca ttgatgcacc tgagccgcga aacaccggaa caaggcctaa
     8221 agggtttttc ggtgccgagt caaccagtta cctccagaat ttgttgaaag ggaaaaaggt
     8281 caggctcgaa tacgatgtcg cacgctatga ccgttacagg cggacactcg cgtatgcatt
     8341 ccttgaggac ggcactttta tcaatgcaga gctggtcaga aacggctatg ccaccgtgat
     8401 gacaatgcct cccaatgtta aatacgctga gacattcaat aagctggcct caaaggcacg
     8461 gaaacaaaag aagggactct ggaaagagtc gccttttgta aaataatcaa aaggaatttg
     8521 tctgatatct tgtcatatga aaaaaatcgc ccttctcatt gcttttgcag ccatcgccac
     8581 aacctcatta tgccaggttg acacaacatc agtttcttat gtagcgtatt ggtcgatagg
     8641 ggattcctac gatttcaggg ttcataagac cagcatgcaa tggagcggtg actcacttat
     8701 aaaaaatgac atttcggagt atatcgccaa cttcgaggtg gttgattcga ccagcacctc
     8761 ctacaccgtg gtctggactt tcagtaatga gcttgccaat acgtgggata tacctgacga
     8821 aatgcttgaa gagctctcga agtactctct gactgaagtg gtatatacca caaccgaact
     8881 tggcgagttc accagtattg agaattggaa ggagctcgga gaaatgatgc gttctatgtt
     8941 ttccgacatc ttcagggtgc tcactaaaga ctccggagag gaatatgaca agctcatgca
     9001 gactgcccgg ccaatcatcg acatgtacag ttcaaaggag ggcgtggaac agatagtctt
     9061 ccctgagctg atgctgtttc atttcccctt cggggcgatg atccctgtgg ctgacaccat
     9121 atcatacgaa gacctgctcc caaaccttct cggtggcgtt ccgataaggg ctgatgcaaa
     9181 gctgtggttc gaagaggtcg attttgaaga agaacgaagc gtactggtac aggagtcgaa
     9241 actgaacact gatgatacga tggatctcct caggagtttg tttgacagaa tggggctgaa
     9301 agacgcatca ctgaccgagg cgctggaaac agctgtttat gagatcaaca ccagcgcccg
     9361 gtacgaatat tactattatc ccggcgttcc cattaatatt gaatctaaga agaatatgac
     9421 ccttgatgtt gccggggaaa gggccagaag ggatgaaacg atcaccatcg agctcctctg
     9481 ataccatgga aaatggtttc agaggctgac ataagatccg gaatgccatt gaaggagtta
     9541 cagccgatat ttattttcct ggctgtcgta ttgctgctgt tgtctgcatc ctgttcgcgc
     9601 aggactgttc ctgctgacgc agtccatggc aaatcagggc gaagtgcagg cggtgttctc
     9661 attaaaacca gtgaatttac cctgattata cctgatgtct ctctcagcca gtcagagacg
     9721 aaacagctgg aacagttcat aaatgcaggg atcggacttt cagttgcctc caacaaggcc
     9781 acccccgaag atatcatcag aacagcacgc ggatatctgg gtgtgccgca ttgcatgggt
     9841 ggtacaacgg cgaagtgcat ggactgttca ggattgatat tcaggatttt tgccaggcat
     9901 ggcataacac ttccgcacaa ctcagaagaa caggccaggt acggcagaat tattactgaa
     9961 aagaagatgc tcatgccggg tgacctggtc ttttttgtca ggacatacag cagcagccgg
    10021 ctgataactc attcgggcat cttcatcggt gaagacaggt tcatccatac ctcatccggc
    10081 aagggggtga caatcactcc gctgaatgat ccgtactgga aggagcggta cctcttcggt
    10141 acaagaatct tcgagtaaaa ctcacgtccc gctgctggtt tccggtatgc agactcctgc
    10201 ccttcctctg tttgtcactt tttcatgaga gtctgcaggg tggtcatcga ggtcacaggc
    10261 agtgccgccg ctgaaatcaa agtcatacta ttcaccctac cggtgccgct gaaatcaaag
    10321 tcatactgtt caccctcccg gcgccgctga aatcaaagtc atactattca ccctcccggn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtcatactat tcaccctccc
    10501 ggcgccgtta aatgatagtc agggaagaac ctgaaatggg ggtgggagaa cttacaaagg
    10561 gacaagatga gccagggaac gaagcagccg gacgacgcca attatggcgg tgactattca
    10621 tatgcaatta aatcagcact tttcggggta gccgttggcg acgcgctggg agtgcccgtc
    10681 aaattcaaaa gcagggagta cctgaatatg cacccggtca ccggcatgac aggcttcggg
    10741 tctcacagcc agccaccggg cacatggtct gacgacagct cgcttacttt ctgtctggca
    10801 gaagcgctga tccgggggta tgacctcgat gccatcggac agaacttcgt gaagtggtat
    10861 tccaacgcgt actggactgc cggcggtgaa gtatttgaca tcggcaaagg aaccgatgag
    10921 gcaattgtgc gtctctccgg ggggacaaag ccggagctgt caggcgatac ttccgtgtca
    10981 tccaacggca acgattcgct gatgcggata ctgcccctgg tgttccggct gatgccgaaa
    11041 ccgctgacag aacgtttcga aataacccgc caggtatctg ccgtcaccaa cggacacata
    11101 cggtcaataa tatcctgctt ttactacctc gagtttgcca ggcagattct tgaaggccgg
    11161 gagaaatatg atatatacag ggatatccgg aaggtcgttt ccggccatct ctcagccctg
    11221 cttattgatc cggcagaggt gttggtattt gaccgcctgc tgaagtatga tatattcagg
    11281 ctgaatgaaa gcgaaatcag caccggcagc aatgtcatca atacactgga ggcagccgtc
    11341 tggtgccttc tcaccacaga gagctatagt gaagcggttc tccgggcagt caatcttggc
    11401 ggcgacaccg acaccacagc agccgtcaca ggcgggctgg caggattgct ctacggtttt
    11461 gacggcatcc cggaagagtg gatcaggcag atcaggcgca aagaggatat tgaagaactg
    11521 gcagaaaagc ttgctgcagg cctgaacggc aactgacaat tttttacatt ttatcctccg
    11581 tggagaccgg atgaacaacc cccacggcag ctaatgcggc acggcaaata aacattgaaa
    11641 tttccggctg gcacatgtcc tgtttctgat ctcttcttcg taaattgctg tcacgattta
    11701 tgagacagtg gcgaggaggt gttcatgcgc cgctcaataa tgtgtttgtt tttcttcagg
    11761 caattttaaa ccagagaacc atgaaaagca gaattctgac agcaatgatc acagcgctgg
    11821 tcacgataat cctgattgct gtggcagtct attttatctt cggcttcaac tccaaaccgg
    11881 gcaaagtcat gtccgtcaat tccccgcagg gatcatttga agcatatgtg attgaaagtc
    11941 cttcggcaga cccgcccaaa caaagtcttt tcattgcaaa agccgggact ggcgagttca
    12001 ggctcgttgc cagcctgcct gaggatattg agtcgacgca gacaatttac tggactgaag
    12061 agggtgacaa agcaatcttc gtaaccaact ggcacctgtt cgtcacggat gtgcagacat
    12121 tcaataccat gaagataagc ctcaatcccg actggtggaa gatgcacgag ggggaaaaga
    12181 ccttttcaag ctccggcacc ccggttgtta tggaagaact catcctggac gggtctgaca
    12241 gcctgaccta caggacgaac ctgatgacgc agcctgtgac cgtaagcctg accggtctct
    12301 gatgatcact attccgtatg gaacaaagag attacctgct tcgggagatt gaaaagatag
    12361 gagccatcat catggtaatc cgccggaaac tctttggcgg gacggatgaa cctgccataa
    12421 cagtggaaaa tcaggctgaa gcattgaagg agatgctgct gagcgaagca ttcattgacc
    12481 tcaatgagct tcttgccatg gatgccgaag acactgataa ataccttgcc gggcacgaag
    12541 gattcaatgt tgcaaatata gagcttctgg cacaaactct ctctgacctg ggaatgacat
    12601 caacgccgcc attctctttc gccctgcttg agaaagcgct gcagctctat aagatatgca
    12661 gcctgcgtga caggacatac tcgtttgaaa gagaagctgc tatcagcact atcagggaag
    12721 ctctgaaggc ctgacagcac gtcagaacac aacataaagc cgcatacgaa gcaactaacc
    12781 tgctcggtat gatcagtgaa ggccagggcc gaagaagcgc tagtacatca ggtggatctt
    12841 ctccttgttc ctccagcctg cctgattctc gtaatccacg aagaaaatct tcaggtcaga
    12901 ctggttctca tactccacga agtatatctt cttctttgcc tgattctcat attttgtaaa
    12961 gaaccacctg ccgttattct cccctgcctg gttctcatac ttcaccttga aaaccttcag
    13021 gtcagcctgg ttttcgtatt taaccacata aaccttcaca tcagcctggt tctcatactt
    13081 tacgctgaat accttctgtg ccggggatgc ggctgaaatc gccagcagaa tcagcgccaa
    13141 tgcaattctt attcctttca tgccttcgtc tatttatgtc tcgttacagc cactccgcct
    13201 ttgtgagaca caacaacgat atactgggca ttatcaacgg aaagacggta gacatcagtt
    13261 gttgaattga tgcttgttat cttgcccgaa tactggggca cagccggttc agatgccggc
    13321 ttatgcgaaa caagcagaaa aacagcgact gctgcagcaa tccctgtaag gattccggaa
    13381 acataattca gcttcttcat gatcttcata tttgcgcgtt actgttatta catggtcaaa
    13441 tttcgtgcaa aaagttaact gccggccagg ggtttggctt cattaaccgg ctctttgggt
    13501 tccaaagctg ataccggcat aaagaggggc ctgaattcat tgaccggctc tttgggtccc
    13561 aaagctgatt ccggcataaa gaggggcctg aattcattcg caggctctgt gggtcccata
    13621 gctgattccg gcataaagag gggcctgaat tcattaaccg gctctgtaga ctttaagtct
    13681 gacgccggga tactcctgac attacggcca ttatctctcc ccatttgagt tcaggcggtt
    13741 gcatgaatga ccggcatgag taatatctat ccttgctgaa tcttaatctg gtgccatgtt
    13801 caacagggta cttatcttca aggtcatcag acagcaattc atcggcctgg ctctcctccc
    13861 aaatcgtctg attcataacc tttgtcccct cttcataaaa atcatctctg tcagtcagtt
    13921 cagattcaag ttcatacttc ctgccggtga gtgtgctcag cttctctatg actatctcca
    13981 tctccgggtg gttcaccttg ccccttattt tcagcaacag ccccagtatc tgctctgcca
    14041 gtcccgaatc ggtgatgcga agagtgagta tcgagtatcc cgggtcagaa tagaaataga
    14101 ggcctccctc ctgctttatc ggggcattga acccaaggat gtcacttcgc atgacacgga
    14161 tatcatcacg gatggtcctc tccgagacag tggtatacct gcccctgtcc gaggcgagag
    14221 cctcggagca cctctcccgg agttcgtcaa tcgaccatct gcgccgtcca ccactgaggc
    14281 aggagttgat tgtcttgtac cttatgaggg cgttcaggtt tgccggcatg attttctttt
    14341 tctattgcag ataggctgca atgccgtggt ttttctttgc ccacagttta aaattaacaa
    14401 ttaatcccat gaaaccctca tgtgtcaaac aatcaaacac gtatctatgt gattttctgt
    14461 catgtgggtt gcataatacc ttaaaatcaa aactgtttta tgatgaaaac aaaagcgatt
    14521 catttcacag ctttcggcaa tgccgctatc gtccaggtca gcagccagcc tctctcgtca
    14581 ttccccttcc tccctgccgg accggccatc aggaacggcg gcctcgtcat ctccgagctg
    14641 actgcctcgg gctctgtagg ccggcttgct gcactcaaca agacggctgg ctggctcctc
    14701 cttaccgatg ccgatatcct caccggtgca aagcagaaca gggttgtaaa caggtctgtg
    14761 ctgctggcgc cgtcgtcaaa gactgtcata gaggtctcgt gcgtggaacg gctccgctgg
    14821 gactaccggt cgacatcgtt cagcaccccc ggatcggttg ccgatccaaa cttgcggagc
    14881 aaaaaagctg gcaccttctc atacatgaag catgagcctg acttccgcaa tacgcaggat
    14941 gaggtatggg atcatgtcag catgaacctg gcatgtgata gagtttacag caagacggag
    15001 aactaccagg agctgctgca atccaggatg gagaagagac accatgacct ccccgaatgc
    15061 agtcccgccg aaggatgcaa cggtctggcc gtcatcctcg acggaaaggt tatctcggct
    15121 gacatcttcg gaaatgagga atcctacagg cactatttcc cgatgcttcg ggatgccgcc
    15181 ttcacccttt cgcgccacgg cgctgactgc gaacagccgg agatgcatga agcttacttc
    15241 agggcgctgg aggcgattga tgagttcagt gatgccaaac gccatcccga ggagaaacac
    15301 cccggggcag gcactctaag catggttgag acggaacggc tggtaggctt cagcctgatg
    15361 cacgggaaag agttgataca caatgcgctc ttcgcaaggc atgatcaggc aacaaaataa
    15421 tctgccggat agcctgccac ggcttattca actccgagga ctctgtatgt ctcttccaga
    15481 gtttcactgc tttcagtgat tatcagaaga ttgtcgccta catgaagagg agtcttagct
    15541 gttggtacga aataagcgtc atcacgtttc accatgacaa ccagtgtgtt ttccggaagc
    15601 ggcagttcca tgaggttttt accattctca agcgcccggg gagtcacaac cagctctgag
    15661 attattgtct tgatgtcacc tgaaaactca acatcaaact cctcgagctt ttttgcctcc
    15721 accggggttt cggcaaggcc gagcagcttt gccaccttcg gaagcgtggt tccctgaaca
    15781 agcagtgaga caagcgtgca gaggaatacg atattgaaca tgagcctggc atgcggaaca
    15841 tcagctgcca gcggaagaat ggcaaaaaca atcggtacag cgcccctcag gccgacccat
    15901 gagacaaaga ctttatccct caccggcatc ttcctgaagg gagcaagaca caggaacaca
    15961 gttaacggcc ttgtaacaaa gatcattata aaactgatta tcaggccggg gatcaacagc
    16021 ggtatcagct cactcgggtt aaccagcagc cccagagtaa ggaacatgat aagctgactc
    16081 atccaggcca ggccgtcaaa gaaatttaag gatgaccgtt tgtggaccag cttcgaattg
    16141 ccgatgacca gtccgcctat gtaaacagcc agaaacccgt tccccttgag aaagtatgta
    16201 agtgaaaaaa tcatcatgca gaatgtaaag acgaggatcg gatagagaga ggtgttttct
    16261 attttgatgt ggttgattat gtaaactgcc agcttcccga ctaaataccc cgaagctgtc
    16321 cctacaataa gctgaagcac aagggttccg gctgcaagcc agtagttcgg ggaagaaccc
    16381 atattgataa tatctataaa ggtgacaacc aggacataag ccatggggtc attgcttcca
    16441 ctctccagct caagcaaggg ccggaggttg tttttgaggt gaagaccctt cgatctcaat
    16501 atggaaaaaa ccgaggctga atccgttgat gacatggttg acgccagcag cagggctgtc
    16561 agcagaccta tgccggaagc accaggcgtc atccccagga gtacccagat agccagaccg
    16621 gtcaggaatg ctgtcatcat cactcctatt gttgcaagga tggccccctg aggaatgacc
    16681 gggcgtatct ccgacatcct tgtatccatt cctccggaaa agaggatgat acagagtgca
    16741 actgtgccga tggtctgcgc gatatgtatg tccccgaatt ctatgccaag gatgtcagag
    16801 ccgcttaaca tcccgacgga caggaaaagc agcagggcgg ggactccgaa tctggagctt
    16861 gccttccctg cataaatgct cacaaggaac agcacggaaa gacccaggag tataagctca
    16921 agctgaatct ccattagcgc caatattctt tacacatcct gacataatat cttccggccg
    16981 gagagcccgt tgttcgtggg ttttctccgg ccggaagcaa aactaaccat ttagacaaaa
    17041 attctacctg ttttttgtct gtaaaatgga ggaaattttt ttctgccact tttccgccag
    17101 ggttctttct ccggcaagtg aatggaaaac cttgttgttg aaaaactcca gcttcttatc
    17161 actcaatgtt ttatcactgt atctaattat gaggttgact gcttctacta cagtcatgct
    17221 cccgttagga gtcttctctg ttctggacgt gatatcaacc ctgcagtcgc ttaacgctga
    17281 cagatcttcc agttcttcct gtattgattc tccggctttc ctgagatata tcagccggcg
    17341 cttttcaggg tcaatgccaa tcgcataggc acccttccat gcatctgtca tggtcagcga
    17401 aagccccttt gcagaacctg aatccttcag cgtcgcaagc aacagcttat ccttccgtac
    17461 attcttgaaa tgcccggcaa tgattggtga aagtatgagc aataccaaac caaaaactat
    17521 tacgtaaccg attattccca tttgttatac aattaaaaat gaaccaaata gctgtcagag
    17581 caccacaaac cagcattaat accacaggca agcggttctt aacagcaatt aactaaatga
    17641 cagattgttg ccaggctgtt gaaaacagcc ggcgccggct cacaggaaat aatgaaacgg
    17701 aaagatcagg tcggttgtag aaacatctat ataaaccgtt ccggcataat cccggaaggc
    17761 actctctttt actgcctcgc aagccccgaa ccagaccggc aggtctgaca taccctgcgc
    17821 cagcggtctg tcacagggag caggcggttg tttgaggaca tcggcaggat tgctgtttcc
    17881 ctcgttcaga atcagcacta ccggatagtt gcccacatcc attaccgtgc gggaaaacgg
    17941 aatgtcttcc tggagcggct cgttataccg cgggtaataa cccctgattc catggcaaag
    18001 aaacaggaat gtcaaaccca gaagtattcc tttgaaaagc ctttgcctga tcatattaca
    18061 aagttagcaa attaagcatg agatcggcag cctggtaact gcaccggata attgtcacat
    18121 tatagtctga accttaagtt cttctcagcg agaaaacagt taccctggtg gcatggcgta
    18181 aagcatatcc tcaggccggg ttcacggtta tcaacaaaga gaactgctca caatggcttt
    18241 tctgaatgga tttcctattt acgtcatcct gtttatcgga ttggttgaag tgaaacctgt
    18301 cagaacagca gcgacaggca ggtaagaccg ccatccattt ttcggaactc cgacatgtca
    18361 agctcgatta tgttgtagcc gagctccagg atctttttct tcaatttcgg gaatcccttt
    18421 gcgatcagga gataatcatt gacccgcagg caattggccg catagtattc atcaggatcc
    18481 agtatgattg tgtttgccgg gtgagccaca tgtgagaata ccggggtgga aataaaattc
    18541 ccgtcaccca ggtatgcgat gtcagatttc agatgcagtc ctgcttccac ccttataacc
    18601 gaggtagtga aaccatgttt tgcaaggatt gcagagagct ggttggcgcc ccaggtattt
    18661 gttctttcgg agatgccaat gaagtaatgg ttctcagccc gcagaatatc tcctccctcc
    18721 atggtgccgg gcgctatgat tgcttctgtt ggcccataac cggccagcac ctgtataata
    18781 gcctcctcct cccctagtct tgtgggagct cccggccgtg atacaacttt caccctgttg
    18841 ttgacaacag ccgtatcctc cacgaaacac ccatccgggt atctgtcatc ggcctccagc
    18901 actgtcagtt tcacaccgca cttaatcagc gcctcacaat atgcggcatg ctgttcgagc
    18961 gctttgccaa aatccggctt acccagtttt gctgttgtta tcccgccggc aaagttcctg
    19021 cccggcctcc ttactattcc tcttgtatac accttattac agtattggat ttatcccggt
    19081 atcggtcacc tcaattttag tcaaattcct gtcaaaatcc aacttgtcac tttattgatc
    19141 ggttaataac tttttaaggt tttaaccttg attgtgttta tctattttaa ggtcatagcc
    19201 tgatgagaaa acaaattcct gttctggaac atgttgatgc aaaggttata tcagcttaat
    19261 acggcagaga atctgttgat cccacctgcc ggatggactt atttcgtcag acacgcatat
    19321 tccgtcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt cagacacgca
    19441 tattccgtca aacacgcacc tggaatgtca atgaggcaac ctggcagcag aatggacatt
    19501 actccacaga gtgtcaggga attcaccttt gcaagtcaac gtcgccgaca agcctctttg
    19561 tctcatcctt cctgttacag taatacacaa gccccgcata ccccctcgta atgaacggat
    19621 tggtgatttc agttttcata ttacggtttt tacgtaacgt aaatggcgta aatcaatttg
    19681 atttcacaaa taaattagta ttcatttttt gtgacaaaaa ctgcttccaa aatgaaagca
    19741 atattttgcg aaaggcacct atttactgtc ctttatgcaa aatatctctt caggttgttc
    19801 ttcatcgcta aagattattc tcccccccgt ctctggtttt gtaccagtcc ggcaatattt
    19861 aaagcgtcaa tttccagcca gaaagtaaag gcatcataac agggaattat tacctttgaa
    19921 taaaaaaatg gataagtgga tttatcagct cagaatcact ttgattgatt caaggcccag
    19981 gatctggcgc aggattctgg tagaggagga cgtacttctc tcagatcttc acaaaatcat
    20041 acagaccact atgggttgga caaactcgca tttgcatcaa ttcataaagg acgaaacctt
    20101 ttatactaaa agaatgcctg atgatgatta ttgggaggag atgaataatg tggactataa
    20161 agggattaaa ctaaatgaga tattgcgttt cgtcaagaat acaatcatct atgaatatga
    20221 ttttggggac ggctgggagc atgaaatact gttggagaag tttctaaaac cggattcaaa
    20281 cgttaaatat ccggtctgca ttgatggcgc catgaaatgt ccacccgaag atgtaggcgg
    20341 aatgggaggt tacatggaca tgctaaaggt ccttcgaaac cccaatcaca gagaatatga
    20401 aagcttcatt aactggcttg gagaagaatt cgaccctaag gaatttgaca tttcaatggt
    20461 taataaacta ttggcaaagc gcaactacgg cctcccccgg ttctgatgct gactccttca
    20521 aacgtggaag aatttgcaaa tatcattaat tgtctgactt gatcaacaaa cctcaatcac
    20581 aggaataaga actgatcccc tgtttctccg tgttggagcc ttttcactgc atttacttat
    20641 tgtatcgtga acatgtatgc tgatatcagg ggattgacaa tgtaaatcga catcaactgt
    20701 ggccttggcg agacagaccc cgcaaccggg cccggcaaca atgagacagt catgcccttc
    20761 atctcttcgg ccagcatagc ctgcgggttc caagcggacg acctgctgac gttatatcga
    20821 acaatgcctc cggcaataag acacggggtg agcatcgtgg cgcacccttg gtaccccgac
    20881 tgccagacct ccggcggtta tgcccgcatt gccaatgtca tcactgttga cctcaccctc
    20941 ctcgcgcaga tgcggccggg agataaaata cttttcaagg agaccttgct tgattttgta
    21001 caatagtctt accttgaatg gcaccagatt atcctgaact acaattgaat gccagagcta
    21061 accggcacag tacggagaag cttcgtctga tatagtattg atatttagaa tcttatgcta
    21121 tacttcatct tgaatctcag tgcgcccgtg tcagaaatgt acccagaatc agcttcaaca
    21181 tattttgaat tccataccag gaatatctca ttaccgggcc ttaatatcca acggaacctt
    21241 gactgtaacc ctatggtgtt gctcgcgttg tcatactgaa caaaattgta aagtgtcagg
    21301 ttcggactga atagtatatt cgcgttgaac tggtaaacat ttgaagtaaa gctatattcc
    21361 ggtagatcga taatattctt tttcaattta catgcaagaa gaaaaggtac actaatcttc
    21421 cagttagccg ctatttcaat ttccttttgt ttgccatcgt agaaatcacc aaagccgtaa
    21481 gtgatttcgc cccaaatatc ccttgccccc tttgtttcaa gttttatttc gttttgccac
    21541 caagagtaac caccagtagt aatgacaaca tcatcgaaaa tattaaagtc ttccttcaga
    21601 tactcatact ggcttaccag tgaataggtt aactgatctc ctgaaagaaa gcgaatacca
    21661 acaggctgca gttttatttc tcttgtctcg aggtcattcg ttgaggcatt tttgataata
    21721 ctgaactcgc caccggaact aatctgcaaa ataccgtatt ttttcaggcg cggtccaaga
    21781 gtaagcctac cataaatctc atttatatct gtccgcggga caaaccccat gcctgcaata
    21841 aagtcttttt ccactcgata ataacccaga ttggctttga taaagtcatt ggggtaatct
    21901 acctggattc catatgaact attattggta tttgccacct ctttagtaaa tgatttctgc
    21961 ccgaacagaa taagtgcggc attcttgtct cccctaaagg tagatgttgc gagtttaaga
    22021 tcaagaccaa ccacaaaatt ggactccggt cccagcgtat taccataggt tcctataaat
    22081 cccactgatg actgtttcaa gaaattgcgg tttaccctag caacataaaa gttatttttt
    22141 tctttttccc tgtcgtcatt gatgtacagg gcacctatat tccagccacc cgccatccca
    22201 gtaaacttta ccgcgtagtt tataggaacg ggttgtccca gtgtatcgag tcccatacgg
    22261 cgtgagaaaa aagttatcac cttatcctgt actgatttag cccctccagt ttcatttcca
    22321 aattgaaaat aattagctcc gtcgagaaag aaatttcttt tatcaggata gtgcagactg
    22381 aacctcgtaa gattaatctg ccgattatca acttccgttt ctgcaaagtc ggtgtttaat
    22441 gagatagcag ttttcaggct tggcgttata tggtagctga tatcaagtcc ggctgagaat
    22501 ctataatccg atccattgtt tcttttctgg tcaaaccctg ttactatgta tggtaaaaag
    22561 ctcaacccta aaccttggga aatatctatt aatccaacta gtattcctgc atcggaaagc
    22621 tgactagatg tgctgttgat accaacctta ggccaataac tggtttcagc cttatattca
    22681 atttccctgg agaatttaat accccatgta tcattattcc tattaaaccc aagggtctgg
    22741 aatggaatag ctatctctgc ctcccaaccc cagtcggtga tatgagccct gccatcccat
    22801 attccatccc aatcctcatt ctttaaagaa ccgtcattgg aaagcgatgc atcgattttt
    22861 gcccccagtg gattgattgc gatataatac ccagttcttt tgttgtgata cgtatcgata
    22921 agaattctca tcctgtcatc attgcttatt ctggcatcat atagcatctc cttggcagca
    22981 attttatcag gattatccca gcatttgatt ccaaagtaga tgttctttga atcatagcat
    23041 acgaagacct cagtcttttc cgaagaaggt tctccgaagt ttggttcctg ctgaaagaat
    23101 tcataaacct taaaagccaa gttccataca tcatcatcca ctctcccgtc aatatgcggg
    23161 ggattgtcaa ctcgtactgc atctatttgc ggctgggccg atgataaaat tgccagagag
    23221 taaaacaggg atagcatgaa tattctttta taaactatgg ttggaataaa ttctaacatt
    23281 caataattaa ttaatcagtt aaggcaatta atttattcat aaggaggatg accacttcat
    23341 ctgtagttcc ggcttttttc cttctatgca accagatgaa cccattattt ttttttgaag
    23401 tatcagccgt gaagtcacca aaaaggttgc ttgctatcta aaaagcaatt gtaccagttc
    23461 agcgcgttaa tgacgccaca ctcttcattc atgaccgcag ggatatgttg tactgatgaa
    23521 gttattgcca tggtgagaag atcgtcccat gccttcttga gaatcaatgc gggtgtcttc
    23581 agcactgcat aaaaaaagag aaagtccagc attttgttta cttatacagg atttttaaaa
    23641 aagtccgggg aattgcttca atttcaacta tcctcccggg caggcttctg aacattccgg
    23701 aactcagcaa acccattgac cagaagacat ctagccttga ccgttctgca ttggctgtta
    23761 tctgtgagcc cattgacaat ccggcgatag ccctgttctt gctaccgtgc aaagtctgaa
    23821 agttcttctc aatgaccggg atcaagcgtc caacgatatt ctgaacgttt ttactgtagt
    23881 ctctctgagt gttgccttct ccacccgggg tactgggagg aacaatgcca ataccacctg
    23941 gagtagtggg agcaacagtg ccaagagctc gagaggcacc acttataact ctgttgccac
    24001 taccgcccat gccgagaggg ggtgcgaagc agggaccagc caggtcaaaa gatgcaagct
    24061 cattccagta tgtattaggc attaccgcga tcataggact ggccttggcc tggacaatga
    24121 gttggtccat gatgatgctg gcaattccca tggcaggcca tgcatcttca tctccgccac
    24181 ggccatgcaa caggtaaagg acagggcagg atgcttcaca tccctcatat cttagaagtc
    24241 tatatataaa tgcatgacta gagaccttta taaatgttga gggtgtccag atcgttgacc
    24301 taattccgtg cgacatttga tatgcttgca taaactctaa catatcacga ggtacaagaa
    24361 cggtattcat gaatcctaca ccgtaacgtg agatgttata attgctagca tctattgtcc
    24421 tgataccatc tactgaaaac gtataggtcc aaagctcaga ttgattgtct ctcgtggtat
    24481 ctttccatac attattgcag cttttgatca tcgctataca ccggccttcc ggccagttca
    24541 catttaccag tacttcattg gtacgagaag catatagcct gaatgtaaca gatacgtcat
    24601 caagcacatc ccaaggtttt acctgtactt ccccgcataa tccaccttct gtaaaagaaa
    24661 aaattgtgcc agattcctgt gcatccagta caaatgaaaa aagcaccaat actgcaattg
    24721 ttttaatcaa tttcatcaca agcctgttct gaaattatat caataatatc agtctgagga
    24781 tgtccttagc attactttta aatggtaggt tcatccggca cataaacacc actttctgta
    24841 taggctatag tactgaaacc cctgatgaac ttatgcatcc tcacccggcc cggtccaccg
    24901 ccaccgctga tatgagtgat tttataatca taacagttgg ggtcgttgat gactgcattc
    24961 acagtttcaa gtaggctgaa cattaaatgg ttttcaaggt tgaccataaa gtgcccgggg
    25021 aaaatgtaat catactcatt aatccgttca acaagttttg tcagggcttt gctgaatgta
    25081 gacaggtttg catatttgcc gccaccattg ccacagcctg taacatcact acaaacattg
    25141 tcaccggcga agaggaagcg gcctttttta tcaaggaaca tcacatgctc gtttgtatgt
    25201 ccgcctgtaa aaatcagctc aacatcataa tcaccaccca agttgaagac atacccgtca
    25261 ggaacaccaa caatctcgta cttcttaaat ggcggtatat cattccggtc aaactcaagc
    25321 caaaggggat tg
//
LOCUS       DAOPBK010000009        24846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2165_length_24846_cov_5.232463, whole genome shotgun sequence.
ACCESSION   DAOPBK010000009 DAOPBK010000000
VERSION     DAOPBK010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24846
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2165_length_24846_cov_5.232463"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1949
                     /locus_tag="PLA17_01230"
     CDS             102..1949
                     /locus_tag="PLA17_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPK84064.1"
                     /translation="MDGNYSEENVITLEWKEHIRLRPGMYIGKLGDGSSLDDGIYVLL
                     KEVMDNAVDEYMMGFGRRIDVSIVGSEVRVRDFGRGIPLGKVLDVSSKMNTGAKYDSK
                     AFKKSVGLNGVGIKAVNALSSSFVIRSFRDRRLKEVEFRRGELVRDEPLKKTEEENGT
                     EVIFVPDEAMFGNFHYIAEYVESMMKNYSYLNAGMVINLNGNKIVSRNGLVDLLNDNM
                     NCEGLYPIIHLKGDDIEVAMTHGTQYGEDYYSFVNGQHTSQGGTHLIAFREAITRTVK
                     EFYKKDFDVSDIRSSIIAAISVKIEEPIFESQTKTKLGSKDMGPGGPSVRNYVVDFIR
                     KHLDDYLHKNSETARIMLKKIQDSEKERKAISSIQKLARERAKKVSLHNRKLRDCRIH
                     YNSNDDRRLESTLFITEGDSASGSITKSRNVETQAVFSLRGKPLNSYGLTKKIVYENE
                     EFNLLQAALNIEESLDDLRYNYVVIATDADVDGMHIRLLLLTFFLQFFPDLVRKGHVY
                     ILQTPLFRVRNKKQTRYCYSDAERQKAIKELGPDPEITRFKGLGEISPDEFVHFIGKD
                     MRLQPVIMKKTDPYTQFLEFYMGKNTPERQDFIIDNLRVEEDIPEAVRV"
     gene            1969..4611
                     /locus_tag="PLA17_01235"
     CDS             1969..4611
                     /locus_tag="PLA17_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPK84065.1"
                     /translation="MKESDSFELIPEEEGGGKEIPESGNIPEALHSVTALSGMYQNWF
                     LDYASYVILERAVPHISDGLKPVQRRILYSMKRMDDGRFNKVANIIGHTMQFHPHGDA
                     SIGDALVQLGQKELLVESQGNWGNILTGDGAAAPRYIEARLSKFALEVAFNSKTTEWM
                     NSYDGRNREPVTLPVKFPLLLAMGVEGIAVGLSSKILPHNFNELIEASINYLQGKPFE
                     LYPDFPTAGMIDVSKYNDGARGGVVKVRARIEKVDKKGLVISEIPFGRTTSSLIESIV
                     KANDRGKIKIKKIDDNTAGGVEIVIQLQSGVSPDKTIDALYAFTDCELSISPNACVIT
                     DENKPAFVGVSDILRHNTDRTVNLLKLELEIRKAELEEEWHFSSLEKIFIEKRIYRDI
                     EECETWEAVITAIDNGLKPYKKLFRREITLDDITRLTEIKIKRISKYDSSRADEHIRG
                     LETDLEEVTNHLANIIPYAINYYRQIRKKHGKGRERRTEIRSFETIEATKVVANNAKL
                     YVNRQEGFIGTGLKKDEYVCDCSDIDDIIVIRKDGTYLITKVEEKQFVGKDVIHAQVF
                     LRNDARTIYNVVYQDGREGPCYVKRCAITGLTRDKEYNLGKGTAGTKILYLSANPNGE
                     AEVIRVYHKPRARLKKVTFDFDFGELAIKGRGSMGNILTRNAVHKITLKEKGLSTLGG
                     RKLWFDDAVMRLNADGRGKFLGEFASDEVLLVVTRGGWYRTSGTDLSSHFEDDILLLE
                     KYRESKIFSAVYWDEEQQFYYVKRFAFEETERLQCFISDSEGSRLVSLTEVEYPRFEI
                     KFGGKNEGRRQEIIEVAEFIGVKSFRAKGKRLTNYSVAGIRELEPVVRSEAAPAPREP
                     EEDTVDKSPQAHMPDDPAQMKLDL"
     gene            4620..5147
                     /locus_tag="PLA17_01240"
     CDS             4620..5147
                     /locus_tag="PLA17_01240"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPK84066.1"
                     /translation="MNIYLIGFMGSGKSSAGKGIAACLRWSFTDLDKLVEEKVGMTVA
                     GIFASKGEEFFRKAEAEALQDVSGQTRRVVACGGGTPCSEKNISLMKATGVTVYLRVT
                     VDELVSRLNRSVTERPLLKDAGPDELHSSVKKLLEKRRSWYEQADLTLDPETMGEEEM
                     TTMIADTVRSKGVCL"
     gene            5222..7549
                     /locus_tag="PLA17_01245"
     CDS             5222..7549
                     /locus_tag="PLA17_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84067.1"
                     /translation="MKTGFNRVFLTALVLMALIAAALVAVLTDMSLFRATPLLYTLTL
                     SVIIIGTGLVIRNRYYLPVLRNLDLRGKLLLAPVVLLALTIPAALLYIYYDLNGRESL
                     LLLSALITSVAAAFFLPSMAMITLPAMASVSWLAEKAGLTSGRIKAEEIVRRTVIAAA
                     FLAVVLVAVTIAGDLRISSIIFLFPVAAIALMLLDIVIMVHFRNRLAMMLEAPVPPET
                     SGADLDQTTAKSEGFMSVVLVADHYPDLIGGRLEYLLNNAGDFYAREVISLASKTYNP
                     ALLPAMKAIASAGRFSDRLRHEAATGVSVIERYYSDPVRNADLLRLPGIPEKAAAARA
                     VFLSKSKPSEQEIIKLLGDASAEVRRTGLMAAGRYGMTSLREEVMKGLDNPDTAREAY
                     YVLRQFGPEVYGEQIGTVIRPGNSERKNYIILRLLDAMPYSEAFPWLSEFVAAGHMGV
                     RLKAASSLCNRGWSPQGRQKHKIEETLSETIHVMARLIAMQTEASRSGYFLLSAALEQ
                     ERANNYELIGCLVRLLAGAAAADLIVPRKRDARPCQAGVASEAIESVIVDPVRRPLKA
                     LLGNSTDSRRLDELSLYFPVRSVKGQSISSFLLASEQNITGTWSKACALHLAADEGRG
                     LDREHAISYLFSNSQILQEESARAIRSIDPGWYRDAEQRLPDPARARISAVMDGSLPQ
                     VAMCFEKTRFLSLCFSGIPEETMIMLASHLHYSGSYDAGSLPGVISWVVPSRDGKTGL
                     YSIPVNDIASFVFYHPEFTDIFVNYTDSQEILPAI"
     gene            7561..10566
                     /locus_tag="PLA17_01250"
     CDS             7561..10566
                     /locus_tag="PLA17_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84068.1"
                     /translation="MPETNIIIRPNPFPGLRAFRPDEGHLFFGRMESTLKVVSKLKEN
                     RFVAVLGASGSGKSSLVMSGVIPALLKENREEKKSWSYLVFRPALNPVDHLAKELSAL
                     SAGAGFNQLSDVSVAASLHNRTEGLTDVINRIRKNLRQQVVIVIDQFEEIFRFSPVTS
                     RGIPGDDATDFIDMIVNAMQKPDHGLYIILTLRSEYVSDCGRFHSLTNLMNSSSYLLP
                     QIPVDQMVTVIEEPVKLSGASIDRSLVKLILSDLEERPGQLPVLQHLMMRIWNHWSRL
                     GDLSHPIGIADYEAVGMLKGAISQHAGQAYESLDERHRYVCSRLFRTITTRSDDGREL
                     SKPERISTIAALTGCAEHEIIDVAEVFRSPEYSFLTPSKEVPLNGESILDLAHESIIR
                     LWGTLRQWMDDEEASVKLYRQLASAAARYQEGTGRLWTAPDLLMALRWRDENNPTLAW
                     AEKIDPAFERALIFLKNSEEEYLIRQEYGKKSSKTVRRSRLITGLLGLLAILTLFALG
                     AVHSLRNRAERQKLVAIRMKDEAVALNERLSDSLEALSSVLGLASAGADGSLIMPDER
                     VRQAEARARSAEEAMRELAYGKAKDMERVSKANSSRMLSLARSLAVRSLNHAGNRDLQ
                     ILLAWQAYLFNRKYDGIAEDADIFAGLYDVSKRYGSRFCARFTPDGAEFTAMAEGSDG
                     IFFTADSKGRVLSWKAGQPSAGYDLVWSGNKTISAMTLSPDRSWLACGTEDSRILMIP
                     FDDDSVGYWLHDSTGTITALHYTDTDHLYSSTSGGSVNEWNLRNRGVRHVTSDNSGIV
                     AMEISDDNNMLAALTGDGRLICWQAGSPEKHVTIKTGDKNITAHKFIPGGYRLATGDD
                     TGAIDIWNTSTGFADRNASGHMAAVRGIAFNGADGQMLTADETGEVRLWTMVNLEQPP
                     VVFSDGGPAVLRIAFIDSGESFLAATQTDVIRRPAHIRLMTDGLCDKVTRNLTEQEWS
                     AYVGGDIEYESTCPDKEYRIRVREIRGAR"
     gene            10666..12186
                     /gene="gpmI"
                     /locus_tag="PLA17_01255"
     CDS             10666..12186
                     /gene="gpmI"
                     /locus_tag="PLA17_01255"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPK84069.1"
                     /translation="MKENRILLMILDGWGIGDGSRSDVISTAGTPKIEALKKAFPSSQ
                     LLTSGEDVGLPQGQMGNSEVGHLNIGAGRIVYQDLVKINKACRTGEIRMNETLRQAFT
                     YAREKGVKVHFMGLLSDGGVHSLDSHLYALCDMTMEYGLKEVYIHGFGDGRDTDPKSG
                     KGYMASLLKHLEKSNGRVASFVGRYYAMDRDKRWERIREAYDMLVHGRGTETGDILRA
                     MQDSYDAGVTDEFMKPIVVTGADGLPLATITEGDVVIFINFRNDRARELTIVLTQQDM
                     PEAGMKTIPLHYLTMTPYDDKFQGLHVIFPKENVDRTLGEILADAGRKQLRIAETEKY
                     AHVTFFFSGGREKEFPGEERILVPSPKVATYDLQPEMSAYGVRDAVIRELGKKKYDFI
                     CLNFANGDMVGHTGIYEAILKAVRTVDECAGDVVKAATANGYEVIIISDHGNADYAIN
                     HDGSPNTAHSLNPVPLYLISKRFKSVRAGILADVAPTILTMMDIPVPPEMTGKSLV"
     gene            12183..12782
                     /locus_tag="PLA17_01260"
     CDS             12183..12782
                     /locus_tag="PLA17_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HPK84070.1"
                     /translation="MRETMLQIDDTIVSLALIEKKFVCDLAACRGSCCRYGDSGAPLT
                     AEEASVLERIWPDLWPWLRPEGIRAIEKQGTSITDFDGELVTPLISNEECAYTIMDDD
                     VYKCGIEAAFNAGAVDFRKPVSCHLFPVRVKSYNTFRAVNYEEWSICKPGVACGAKQN
                     TELYIFLREPLIRAFGEEWYNRLHWAAGEYHKYNDNGKH"
     gene            12769..14145
                     /locus_tag="PLA17_01265"
     CDS             12769..14145
                     /locus_tag="PLA17_01265"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPK84071.1"
                     /translation="MESIKNYIETNRDRFLEELFGLIRIPSVSASESNKADMVRAAEY
                     IRDLMAANGADRAEVYSTPGNPIVYGEKMVDPSLPTILVYGHYDVMPAEPLEKWISNP
                     FEPEIRDGKIWARGADDDKGQLFMHLKAFEMMNATGTLACNVKFMLEGEEEAGSTSLP
                     GFCTEYREMLRSDVILVSDTTMISPETPSITSGLRGLSYIQVEVEGPDHDLHSGIYGG
                     AVVNPCNALCDIIARLKDSDGRVTIPGFYDSVHVPSDAERAKMAERPFDPEKYMKSIG
                     VDALAEEKGFSTLESTGIRPTLDVNGIWGGYTGEGAKTILPAKASAKISMRLVPYQKA
                     EEIAELAINHIKSLAPAGVKVSAEYLHGGEAYVSPIDTPEYLAAEKAYTDIFGKKPIP
                     IRSGGSIPIISTFERILGVKSILMGFGLESDAIHSPNENYPLENLFAGMRAIPLYFKY
                     YAELKKKG"
     assembly_gap    14199..14298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14418..15677)
                     /locus_tag="PLA17_01270"
     CDS             complement(14418..15677)
                     /locus_tag="PLA17_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPK84072.1"
                     /translation="MNPWVIIPFAILLAAFFSAMELAFIASNKLRIELDRKQGNFGSG
                     IIAIFTRNPGLYISTMLIGVNIATVIFSMLAADLMQSWFENLIPSEVLIFIIQTVITA
                     MIILVFADFLPKSVVRISPNFFLRLFAVPTAVFYYLFYPVSKFTLWIANIIMRVFFGI
                     KTERKEQENRIFTRIDLDHFVNDMVETDEEKEEEISSLRIFQNALDFSSVKARECMVP
                     RTEIVALPVHSSPDELRHQFIESGHSKILLYRNNIDNMVGYYELKDILHGPADIATDM
                     RKLPVVPETMTANRVLRLLSDAKKSIALVVDEFGGTAGVITIEDVLEEIVGDIEDEHD
                     TSDLVEKKINEKEYVLSGRLDIDYLNEEYDLDLPESDEYTTLAGLILFAHGNIPRQQE
                     KIRIGHNLFTVLKSSSTKVELLKLQID"
     gene            complement(15687..15869)
                     /locus_tag="PLA17_01275"
     CDS             complement(15687..15869)
                     /locus_tag="PLA17_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84073.1"
                     /translation="MNRKSLLLIEIIWIILGLMCLGIAVRETVNAGIGRAWLFYVMAV
                     AAFVLAWIRDSQRKKL"
     gene            complement(15841..16392)
                     /gene="lptC"
                     /locus_tag="PLA17_01280"
     CDS             complement(15841..16392)
                     /gene="lptC"
                     /locus_tag="PLA17_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPK84074.1"
                     /translation="MIAVLIAGTALFSSCDKKEITVKDLMIKGLPTLSARNVETMYSD
                     SGKVTMVVQTPLIQQYSSEENPYTLFPKGLTVLFYDRTDKPQASITSNYARYTEKDDV
                     WELRDSVVAVSNKGEVLETELLFWSQPRDRIWSDRFVRFTHNDQIVMGTGFNSDSRFS
                     NWTIKNVTGTIYIRDEQEVTPAD"
     gene            complement(16425..17753)
                     /locus_tag="PLA17_01285"
     CDS             complement(16425..17753)
                     /locus_tag="PLA17_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84075.1"
                     /translation="MKRTTSTILTGLLLSVFSFTLQAQMGIETGTPFGKGEDSTRCLR
                     NTSLYSTYYDNKDYNMAVQFWRLVFNECPASSKNTYIKGETMYKEFFRKTKDNAYLDT
                     VLMILDQRTRYFNEEPANNLRKAFALYEFGGNDVEYTSKCYDLIKGVMETSPESFDHT
                     YSSLYMAVIAKSYALRLIEAPEIITAYSQSMKVVDNQLSRQPGDTRYLEARKNIDAIF
                     RSTGAATCENLEHLFTADVDNNPGDTAMLRKVLTLLTETGCKDSELYYKASTNLYRAD
                     PSASSAAQLAEMNLVRKKYNEAEKYYTEAIELETDNKVKSVLLTKLATLELMSDSRQA
                     ARDFARAAYSLDPTNGNALFIIAEAYAGSRIGETFENQTVYWVVVDYLVRARNTDPTL
                     KEQIDERISIYSRLFPTKEEAFFRSLVEEGASYQVGGWINESTTIRFRKE"
     gene            complement(17772..19019)
                     /locus_tag="PLA17_01290"
     CDS             complement(17772..19019)
                     /locus_tag="PLA17_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84076.1"
                     /translation="MISLLALLATGVAGQKKVNTPFARYGIGNLELQGNSRTLAMGGI
                     SSGIRDNLTLNYLTPASYSSTDTTSFLFDFGIDYAMAKLKGDGLRYYSQDITFAHLML
                     GFPIMKGWGVAAAVMPYANGSYSIGYRSEGSGVTGSLYELHNGSGGFQKALFGTGLRL
                     LPFVSAGANIFYMFGEETRINDFAFTADNNHFNTRKQGTSGMRGFGYDASVQFMLNLS
                     GGRFIIAGLTYTPAYDLKTNNEDVTMRYSTASTSIFSIDTLTHAKLSTTSRMPQSFRA
                     GFSLGKSDKLTAGADVVYTRWTKASLPGTYGTYIDALSLHAGAEYIPERYSNYSFFDR
                     IEYRIGCRYGEGYTLFGADELKEYGITFGTGIPMKRSRSRISLMVDLSTRGGNGSIPR
                     ETRISVGASLNLYDYWFIKRQYD"
     gene            complement(19036..19755)
                     /locus_tag="PLA17_01295"
     CDS             complement(19036..19755)
                     /locus_tag="PLA17_01295"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPK84077.1"
                     /translation="MNLIIDIGNTSVKAALVSEGVIISQERFDSADPEMLGMFTGRRR
                     LSGAIVSSVSGDQSELFDWLRTRAGRVHHLTSRSLYPFAIDYMTPETLGTDRLAAAAG
                     AVNHHPGADLLVIDAGSALTVDQVTGGVYRGGSISPGLAMRFRALHEFTGKLPLVTKR
                     TGFTVPGKTTEDAIAGGVITGLVYEIIEYIRTFKEKYHNSVAVITGGDSGLVTPLAGG
                     RLLHYPDLVTEGLNFLLETNV"
     gene            complement(19759..23136)
                     /gene="mfd"
                     /locus_tag="PLA17_01300"
     CDS             complement(19759..23136)
                     /gene="mfd"
                     /locus_tag="PLA17_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPK84078.1"
                     /translation="MAGNVFREIYRRHPSFRRLEEMLAAHSAEPVTIHGLTGSSRSIV
                     MAGALSQQPTTHVVLLPEKEEAAYFYNDLTSLMGDNNIFFFPGTYKRSVMYEQTEPAN
                     IILRAEVLNFLSSGRRKCIIVSYPESVMEKVISKTSLKKNTFFIKKGAKLSIEFLEEV
                     LREYNFQRTDFVYEPGQYAIRGSITDLFSYSTDKPYRIDFFGEEVDSIRSFSPDDQLS
                     LQLHDEIQVIPDIQLLAFQENADSFIDFIPPSSIIWSDSFDYIVEKTDNIWEQACSRS
                     LKGEISDRSDIIITGRHLEELCSRHRRIFFGRGRHNPDTVNIAFRTEPQPAFNKNFGL
                     LASKLASNVQDGYTNFIISESEVQKQRLKEIFEESDPAVQFTPLLLNLHQGFTDHDLK
                     IAVYTDHQIFDRYHKFRIRGFFTRRDSMTVRELTDLNPGDYVVHTDHGIGRFGGLEKI
                     EVNGRMQEAIKLVFKDNDILYVGIHALHRISRYKGRDDTPPRINKLGTGAWQRVKQAT
                     KKKVKDIARDLILLYAKRKASGGFAYSPDSYLQRELEASFIWEDTPDQLAGTMAVKED
                     METPVPMDRLICGDVGFGKTEIAIRAAFKAATDGKQTAVLVPTTILALQHFRTFSSRL
                     ENFPCKVDYISRHRKPAEQKKILKQLADGEIDIIVGTHKLTGKEVKFKDLGLLIIDEE
                     QRFGVAAKEKLKTLRTGVDTLTLTATPIPRTLQFSLMGARDLSVINTPPPNRMPIITE
                     LHGFNEDIIREGIVYEVSRNGQVFFIHNRIENIMEVKDSLLRFCPEIKIAVVHGRMEG
                     EEIENVMSDFIHGDYDVLLATTIIESGLDIPNVNTIFINDAHSFGLSELHQLRGRVGR
                     SNRRAFCYLLTPPLSVLTGDARRRLKAIEENYELGSGFNIALQDLDIRGAGNLLGGEQ
                     SGFIADVGYDTYQKILDEAVRELKDEEFGDLFTAPAEKREHAAGEKEKQFVEDVTVET
                     DLEIMFPDAYVSNIPERIRLYKELNEIRDEAGLKAYESHLTDRFGLPPPPALALLQLV
                     RLKWAAARTGIEKVILKNNTLIANFVTDQSSQFYRSPLFVSVMNYVNRRQSNMSVKQN
                     NNKLSLTVRSVTTVVQAIDLFTEILAWHESAQAVKAN"
     assembly_gap    23337..23436
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23478..24482)
                     /gene="fbp"
                     /locus_tag="PLA17_01305"
     CDS             complement(23478..24482)
                     /gene="fbp"
                     /locus_tag="PLA17_01305"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HPK84079.1"
                     /translation="MPSYDITSLNDFIISKQKDFPFAQGELSSLLHHIGTAAKMVNAK
                     IRKAGLVEILGRVGIENPQGEEQQKLDRYADEVFIDALKASGQCAGIASEENQNEVGF
                     TGEFASRGKYVVCIDPLDGSSNIDVNVSVGSIFSIYRRITERDEKVSIEDFLQEGSKQ
                     VAAGYVIYGSSTMLVYTTGNGVNGFTLDPTIGEFCLSHPNMRTPETGKIYSINEGHYI
                     KFPDWVKKYIKYCQENDPDTSRPYLSRYIGSLVSDVHRNMLKGGIFLYPPSITYPSGK
                     LRLIYECNPISFIIEQAGGVTTDGTGRILSLKPESLHQRTPFYCGSPDMIRTLVGMCQ
                     "
     gene            complement(24511..>24846)
                     /locus_tag="PLA17_01310"
     CDS             complement(24511..>24846)
                     /locus_tag="PLA17_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84080.1"
                     /translation="EGDPWEVEKVAGYNGGSLVDGIFSSIYDGTMTVYDYHSGEPLKP
                     ADVKKIEGEFKNDRTKIGKISFTEDWYYFPEENRVEKRTKSVTFGYELYNNVGKVYAY
                     RAAFRADLN"
BASE COUNT         5979 a         6075 c         6597 g         5995 t
ORIGIN      
        1 ccaactgctg ctgccggcag ggtataatta ccatctgttg ttgataagtc cccgggcatc
       61 gccttgtcta attctttata tttgccccaa aatttgggat gatggacggc aattattctg
      121 aggagaatgt tataaccctt gaatggaagg agcatatcag gctgcgtcct ggtatgtaca
      181 tcggcaagct gggtgacggc tcctcgttag atgatggtat atatgtcctg ctgaaggagg
      241 tgatggataa cgcggttgac gagtatatga tgggtttcgg ccgcaggatt gacgtcagca
      301 tcgtcggttc ggaggtgagg gtccgtgact tcggccgcgg cattcccctc ggaaaggtac
      361 ttgatgtctc ctcaaagatg aataccggag ccaaatatga ctcaaaggca ttcaagaaat
      421 ctgtaggcct caacggtgtg ggtatcaaag ccgtcaatgc cctctcatca tcttttgtta
      481 tcagatcatt ccgtgacagg cggctgaagg aggttgagtt caggcgcggt gagctggtca
      541 gggatgagcc cctgaaaaaa actgaagagg agaacggcac agaggtgata ttcgtgcctg
      601 atgaagccat gttcggcaac ttccattaca ttgccgagta tgttgagagc atgatgaaga
      661 actactcata cctcaatgcg ggtatggtca tcaacctcaa cggcaataag atagtctcac
      721 gcaacggact cgttgacctc ctcaatgaca atatgaactg cgagggcctt taccccatca
      781 tccacctgaa gggtgatgac atagaggtgg ccatgacgca cggaacccag tacggagaag
      841 attattacag ctttgtaaac ggtcagcata cctcgcaggg aggaacacac cttattgcgt
      901 tccgcgaagc gataacccgc accgtcaagg agttctacaa aaaggacttt gacgtttccg
      961 atataagatc atcaattatc gcggccatct cggtcaagat agaggaacct atctttgaat
     1021 cgcagaccaa gaccaagctg ggatcgaagg atatgggacc gggaggcccc tcggtacgca
     1081 actatgtggt tgacttcatc cgcaagcacc tcgacgatta cctgcacaag aacagtgaga
     1141 cagcccggat catgctgaag aagattcagg attcggagaa ggagcggaag gccatcagct
     1201 caatacaaaa gctggcgcgc gaacgtgcga agaaggtgag ccttcataac cgcaaactgc
     1261 gtgactgccg tatccactac aattcaaacg atgatcgccg gctggaatca accctcttca
     1321 tcaccgaggg cgactcggca agcggttcca tcacaaagtc gcgcaacgtg gagacccagg
     1381 ctgtcttcag cctccggggc aagccgctca acagctacgg cctgacaaag aagatagttt
     1441 acgagaacga ggagttcaac ctgttgcagg cagctctcaa cattgaggag tctctcgatg
     1501 acctccgtta caactatgtg gtgatagcca cggatgcgga tgtggacggg atgcatatca
     1561 ggctgctgct cctgaccttc ttcctgcagt tcttccctga tctcgtccgc aagggccatg
     1621 tttacatact gcagactccg cttttcaggg tgcgcaacaa gaaacagaca cgttactgct
     1681 actccgatgc ggagaggcag aaagcaataa aggagctcgg tcctgacccc gagatcaccc
     1741 gcttcaaggg gctgggtgag atatctccgg atgagtttgt tcatttcatc ggaaaagata
     1801 tgcgactgca acctgtgatc atgaagaaaa cagatccgta tacccagttc cttgagtttt
     1861 atatgggcaa gaatactcct gaacgtcagg attttatcat tgacaacctg cgggttgagg
     1921 aggatattcc ggaagcagtc cgggtgtgag tgaaggtttc tgcagatgat gaaagaaagt
     1981 gattcttttg aactgatccc tgaagaagag ggagggggta aggaaatacc tgaatccggt
     2041 aatatacctg aggccctgca tagtgtcacg gctctctcgg gcatgtacca gaactggttc
     2101 cttgattatg cgtcatatgt gatccttgag cgtgccgtgc cccatatttc tgacgggctt
     2161 aaaccggtgc agcgccgtat attgtactct atgaagcgca tggatgacgg gcggttcaac
     2221 aaggttgcaa atatcatcgg gcatacaatg cagtttcatc ctcacggtga tgcctccatt
     2281 ggcgatgcac ttgtacagct gggacagaag gagctccttg tggagtcaca gggaaactgg
     2341 ggaaacattc tgacaggtga tggtgctgcg gcacccaggt atatcgaagc caggctttca
     2401 aagttcgccc tggaggtggc cttcaacagc aagaccaccg agtggatgaa ctcatatgac
     2461 gggcgtaaca gggaacccgt tacacttccc gttaagttcc cgctgctcct ggcaatgggt
     2521 gtggagggca tcgccgtagg cctctcttca aagattctcc ctcacaactt caatgaactg
     2581 atagaggctt caataaatta tctgcagggc aaacccttcg agctgtaccc tgacttcccg
     2641 acagcaggca tgattgatgt gtcaaaatac aatgacggag cccgcggagg ggtggtcaag
     2701 gtcagggcca ggatcgaaaa ggttgacaaa aaaggactgg taatatccga gattcctttc
     2761 ggacgcacca ccagctcgct catcgagtca atagtcaagg ccaatgacag aggcaagatc
     2821 aaaattaaaa agattgatga caacaccgcc gggggagtgg agatagtcat tcagctgcag
     2881 agcggtgtct cgcctgacaa aaccattgat gcactctatg ccttcactga ctgtgaactc
     2941 tcaatctcgc ccaacgcatg cgtcatcact gacgaaaaca agcctgcgtt cgttggcgtg
     3001 agtgatatcc tcagacacaa tactgaccgg acggtaaatc ttctcaaact ggagcttgag
     3061 atacgcaagg ccgagctgga ggaggagtgg cacttctcat cgcttgaaaa gattttcatt
     3121 gagaagagaa tttaccgtga tattgaggag tgtgagacct gggaggctgt aataaccgcc
     3181 attgacaatg ggctgaagcc atataaaaag cttttcaggc gcgagatcac ccttgatgac
     3241 atcaccaggc tcacggagat aaagatcaag cgtatctcga aatatgattc ctcacgtgcc
     3301 gatgaacata tcagaggcct tgagacagac ctggaggagg tcaccaacca tcttgccaac
     3361 atcatcccct atgctatcaa ctactatcgt cagatcagga aaaagcacgg caagggcagg
     3421 gagcgcagga cagagataag gagcttcgag accattgagg ccaccaaggt ggttgccaac
     3481 aatgcaaaac tttacgtgaa ccgccaggag ggattcatcg ggacggggct taaaaaggat
     3541 gagtatgtgt gtgattgttc tgacattgat gatatcattg ttatcaggaa ggatggcact
     3601 tatctgatca ccaaggttga agagaagcag ttcgtcggaa aggatgtcat tcacgcgcag
     3661 gtatttctca ggaatgatgc gcgcaccatt tacaatgtgg tataccagga tggccgggaa
     3721 gggccctgtt atgtcaagcg ttgtgcaatc acagggctta cgcgcgacaa ggaatacaac
     3781 ctcgggaaag gcacagccgg gacgaagatc ctctatctca gcgccaatcc caacggtgag
     3841 gctgaggtga taagggtgta ccataaaccc agggccaggc tgaagaaggt gacctttgat
     3901 tttgacttcg gggagctggc aatcaaggga cgtggttcaa tgggaaatat ccttacaagg
     3961 aatgcggttc acaagatcac ccttaaggag aagggtctct cgacccttgg tggccgcaag
     4021 ctgtggtttg atgatgcggt gatgaggctg aatgccgatg gcaggggaaa attcctgggt
     4081 gagtttgctt ccgatgaggt gctgctcgtg gtcacccggg gcggttggta ccggacctcc
     4141 ggcactgatc tctcatcaca ttttgaggat gacatcctgt tgcttgagaa gtaccgggag
     4201 tcaaagatct tctccgccgt gtactgggat gaggagcagc agttttacta tgtcaagcgg
     4261 tttgcctttg aggagacgga gaggcttcag tgtttcatct ctgacagtga ggggtcacgg
     4321 ctggtgagcc tcaccgaggt ggagtatccc aggtttgaga taaaattcgg cggtaagaac
     4381 gaggggcgca ggcaggagat aatagaagtg gcggagttca taggtgtaaa gagtttccgt
     4441 gccaaaggga aaaggcttac caactacagt gttgccggta tcagggagct tgagcccgtg
     4501 gtcagaagtg aggcagcacc ggcaccccgc gaaccggagg aggacactgt ggataagtcg
     4561 ccgcaggctc acatgcctga tgatccggcc cagatgaaac tggatctcta gatatatcag
     4621 tgaatattta cctgatagga ttcatgggca gcggaaaaag ctccgccggg aagggtattg
     4681 ccgcatgtct caggtggagt tttaccgacc ttgacaaact tgttgaggag aaggtgggga
     4741 tgacggtagc cggtattttt gcctcgaagg gtgaggagtt tttccggaag gctgaggcag
     4801 aggcgctgca ggatgtgtca ggtcagacca ggagagtggt agcctgtgga ggtggcactc
     4861 cctgttctga aaaaaacatt tcgctcatga aggcaaccgg ggtaaccgtc tacctgagag
     4921 tgaccgttga tgagctggtg tcacgcctga accggtctgt gacggagcgc cccctgctga
     4981 aagatgcagg gcctgatgag cttcattcaa gcgtgaagaa gctcctggaa aaaagaagat
     5041 catggtatga gcaggctgac ctgaccctgg atccggagac catgggagag gaagagatga
     5101 ccacgatgat tgcagacact gtaagatcaa agggtgtctg tttgtaggtt tcattttttc
     5161 cccgtacctt tattaagaaa gtctatactt aaacccggat atgtcaaaac ctcggagggc
     5221 tatgaaaacc ggtttcaacc gggtcttcct cacggcattg gtcctgatgg cactaatcgc
     5281 cgcagccctt gttgcagtgc tgactgacat gtctctcttc cgcgccacac cgctcctcta
     5341 cactctgact ctctcggtaa taatcatcgg aacaggcctg gtaatcagga accgttacta
     5401 tctgcccgta cttcgcaacc ttgaccttcg cggcaagctg ctactggcgc cggtggtgct
     5461 gctggcactg accattccgg cagccctgtt atatatctat tatgacctca atggcagaga
     5521 gtcgctgctg ctgctgtcag cactcatcac atcagttgca gccgccttct tcctcccgtc
     5581 gatggctatg ataaccctgc cagcgatggc atccgtgagc tggctggctg aaaaggccgg
     5641 gttgacgtca ggcaggataa aggcagaaga gattgtcagg cggacggtca ttgctgccgc
     5701 attcctggcc gtagttttag tggcagtaac aatagctgga gatttgagga tttccagcat
     5761 tatcttcctt tttccggtgg ctgcgattgc gctgatgctt cttgacatcg tcatcatggt
     5821 tcacttccgg aaccggcttg ccatgatgct ggaggccccg gttcctccgg aaacctccgg
     5881 agcagacctg gaccagacga cggcgaagtc agagggcttc atgagcgtgg tgctcgttgc
     5941 tgaccattac cctgacctga ttggcggcag acttgagtac ctgttaaaca atgcgggtga
     6001 tttctatgcc agggaagtta tttcactggc ttccaaaacg tataacccgg cacttcttcc
     6061 agccatgaag gctattgcct ccgccggccg tttcagcgac aggttgaggc atgaagctgc
     6121 caccggggtt tcagtgattg aaaggtatta ttctgacccg gtcaggaatg cagatctttt
     6181 acgccttccg ggtatcccgg agaaggctgc cgccgccagg gcggtatttt tgagtaaaag
     6241 caaaccttcg gagcaggaga ttatcaagct cctcggtgat gccagcgctg aagtaaggag
     6301 aacgggtctg atggcagcgg gccgttacgg catgacgtcg cttcgggaag aggtgatgaa
     6361 ggggctggat aacccggata ctgcccgcga agcctattat gtgctcaggc agttcggtcc
     6421 ggaggtgtac ggtgaacaga ttggaacagt tatcaggccc ggtaacagtg aacggaagaa
     6481 ctacattatc ctccggcttc tggatgccat gccgtacagc gaggcatttc cgtggctcag
     6541 cgaatttgtc gctgccggtc acatgggcgt ccgccttaag gcagcttcct ccctctgcaa
     6601 cagaggatgg tcaccgcagg gaaggcaaaa gcacaagatc gaagagacac tcagtgaaac
     6661 gattcatgtc atggcaaggc ttatcgccat gcagactgag gcatcaagat cgggatattt
     6721 cctcctgtcg gcagcccttg aacaggagag ggctaataac tatgaactca tcgggtgtct
     6781 ggttcgtctc cttgcaggag cagcggctgc tgacttaata gttccgcgca aacgggatgc
     6841 cagaccatgc caggccggtg tcgcctcgga ggcaatagaa agtgtaatag tggatccggt
     6901 gcgcagaccg cttaaggcgc ttctgggaaa cagtactgac agccgcaggc tggacgagct
     6961 ttcactttac tttccggtca ggagcgtgaa aggccaatca atttcatcat ttctcctggc
     7021 ttcagagcag aatatcaccg gcacctggag caaagcgtgc gcccttcacc tggcagctga
     7081 tgaaggcaga ggcctcgaca gggaacatgc aatctcatac ctgttcagca acagtcagat
     7141 actgcaggag gagagcgccc gcgccatcag gtcaattgac cctggctggt acagggatgc
     7201 ggagcagcgc ctccctgacc cggcaagggc aagaatatca gcagtcatgg atggctccct
     7261 tccgcaggtg gcaatgtgct tcgagaagac aaggtttctg agcctctgtt tttccggaat
     7321 acctgaagaa accatgatca tgctggcctc acacctccat tactcggggt catatgatgc
     7381 tggttccctg ccaggggtga tttcatgggt ggtgccctcc cgcgacggga agaccggcct
     7441 ttactctata ccggtaaatg atattgcctc ctttgtgttt taccatccgg aattcactga
     7501 tatctttgta aactatacag atagccagga gatattacct gcaatttaaa aatgttttct
     7561 atgccagaaa cgaatattat tatcaggcca aatccatttc cggggctgag agcattcaga
     7621 cctgacgaag ggcacctctt cttcggacga atggagagca ctctcaaggt tgtttccaag
     7681 cttaaggaaa acagatttgt tgccgtattg ggtgcctcgg gcagcggcaa atcatcactt
     7741 gtcatgtcag gagtaattcc tgctcttctc aaggaaaaca gagaagaaaa aaaatcctgg
     7801 tcatatcttg tcttcagacc tgcactcaat ccggttgacc acctggcaaa ggagttgtca
     7861 gcactctccg caggagcagg attcaaccag ctgtcagatg tgagcgtggc ggcatcactg
     7921 cataacagaa ctgaagggct gactgacgtg atcaaccgta tcaggaagaa cctccgccag
     7981 caggtggtga tagtcatcga ccagtttgaa gagatcttcc ggttcagccc ggtaacttcc
     8041 agaggcatcc ccggtgatga tgccaccgac ttcattgaca tgatagtcaa tgctatgcag
     8101 aaacctgacc atgggctgta cattattctg acactcagat ctgaatatgt ctctgactgc
     8161 gggcgttttc attctctgac aaacctgatg aacagcagca gttaccttct gccacagatt
     8221 cccgttgacc agatggttac tgtgattgaa gagcctgtga agctttcggg agcttcaatt
     8281 gacaggtcgc tggtgaagct tatcctgagt gatcttgaag agaggccggg gcagctgccg
     8341 gtattgcagc acctgatgat gcgtatctgg aaccactgga gcaggctggg tgacctgagc
     8401 caccccattg gtattgctga ttatgaagct gtcgggatgc tgaagggagc catctcccag
     8461 catgccgggc aggcctatga gtcactggat gagaggcacc gttatgtctg ttcacggctc
     8521 ttccgcacca tcacaacacg ctcggatgac ggcagggaac tgagtaaacc cgagcgtatt
     8581 tcaacaattg cagcgctgac ggggtgcgct gagcatgaaa tcattgatgt agccgaagtg
     8641 ttcaggtcac ctgaatattc attcctgacc ccttcaaagg aggtgccgtt gaacggggaa
     8701 agcatccttg acctggccca tgaaagcatc ataaggctct gggggacatt gcggcaatgg
     8761 atggatgatg aggaagcatc agtaaagctt tacaggcagc tggcctctgc agctgcacgg
     8821 taccaggagg gtacaggccg gctgtggaca gctcctgacc tgctgatggc cctgaggtgg
     8881 agagatgaaa acaatcccac cctcgcctgg gctgaaaaga ttgatccagc tttcgaaaga
     8941 gccctcatct tcctgaaaaa ctccgaggaa gagtatctca tacggcagga atacggaaaa
     9001 aaaagcagta aaactgtcag gcgttcacgc ctgataacag ggttgctggg tttactggcc
     9061 attcttacgc tgtttgccct gggtgctgtt cattcactca gaaacagggc tgaaaggcaa
     9121 aaactggttg ccatacggat gaaggacgag gccgttgccc tgaacgagag gctcagtgac
     9181 agcctggagg cattgtcgtc agtcctggga ttggcttctg ccggtgcaga cggcagtctg
     9241 atcatgcctg atgagagggt gagacaggct gaagcacggg caagatcagc cgaggaggca
     9301 atgagggagt tggcttacgg caaggctaaa gatatggaga gagtatcgaa ggcaaacagc
     9361 agccggatgc tctcactggc caggtcactg gctgtgaggt cactgaacca tgcagggaac
     9421 agggaccttc agatattgct ggcatggcag gcttatctct ttaaccgtaa gtatgatggc
     9481 attgctgagg atgcagatat ctttgccggt ctctacgatg tttcaaaacg ttacggaagc
     9541 aggttctgtg caaggtttac ccctgacgga gctgagttta ccgctatggc tgaaggctct
     9601 gacggcatat tctttaccgc tgattccaaa ggcagagtgc tgagctggaa agcaggtcag
     9661 ccatctgcag gatatgatct ggtatggtcg ggaaacaaaa ccatcagcgc catgactctc
     9721 agccctgaca gatcatggct cgcctgcgga acggaagatt cgagaatcct tatgatacca
     9781 tttgatgatg attcggttgg ttactggtta catgactcta ccggcacaat aacagccctg
     9841 cactatactg acactgacca tctttactct tcaacctcag ggggcagtgt gaacgaatgg
     9901 aatctcagaa accgaggagt gagacatgtg accagtgata attcaggaat agtggcgatg
     9961 gaaatatcgg atgacaataa tatgctggca gccctgacgg gcgatggcag gcttatctgc
    10021 tggcaggcgg ggtcacctga aaagcatgtc accataaaaa cgggagataa aaacataact
    10081 gctcataaat tcattccggg gggatacagg ctggcaacgg gtgatgatac cggcgccatt
    10141 gatatatgga acacctctac cggttttgct gacagaaatg cgtcaggtca catggcagca
    10201 gtgaggggta ttgccttcaa cggggctgac gggcagatgc tgacggctga tgagacagga
    10261 gaggtcaggc tatggacgat ggttaacctg gagcagcctc ccgtggtctt ttccgacgga
    10321 gggcctgcag ttttgcgcat tgccttcatt gacagcggag agtcattcct tgctgccaca
    10381 cagacagatg tcatcaggcg ccccgcacac atcaggctca tgactgacgg cctctgtgac
    10441 aaggtcacaa gaaacctcac agaacaggaa tggtcagctt atgtgggagg ggatatagaa
    10501 tacgagtcca cctgccccga taaggaatac cgtatcagag tcagggagat caggggtgcc
    10561 cgctgattca aactatatag cttttgccgt gttataataa tgcgatggca attttgccgg
    10621 aaatggctat ttttgcagcc agcttaaaaa ataattatta atttgatgaa agaaaacaga
    10681 atactactga tgatcctcga cggctggggg attggtgacg gctccagaag cgacgtgatc
    10741 agcacagccg gaacaccgaa aattgaagcc ctgaagaagg ccttcccctc gtctcaactg
    10801 ctcacctccg gcgaagacgt gggtcttccc caaggtcaga tgggcaactc ggaagtaggt
    10861 cacctgaata taggggccgg acggattgtc taccaggacc tggtcaagat aaacaaagca
    10921 tgccgcacag gcgagatacg catgaacgaa acgttgcgcc aggcattcac ctatgcccgt
    10981 gaaaaagggg taaaggttca cttcatggga ctgctctctg atggtggggt tcattcgctc
    11041 gacagtcacc tctatgcact gtgtgacatg accatggagt atggcctgaa ggaggtttat
    11101 atccatggct tcggcgatgg ccgtgacact gacccgaaga gcgggaaggg atatatggcg
    11161 agcctgctca agcatctgga aaaaagcaac ggcagagtgg catcttttgt aggccgttac
    11221 tatgccatgg accgtgacaa gagatgggaa cggatcaggg aggcatatga catgcttgtt
    11281 cacggcaggg gaacagagac cggggatata ctccgggcaa tgcaggattc ctatgatgcc
    11341 ggagtcacag atgaattcat gaagcctatt gtggtaaccg gagctgacgg actgcctctg
    11401 gcaaccatca cggaaggcga tgtggttatc ttcatcaatt tcagaaatga cagggcccgt
    11461 gagctgacga tagtgcttac gcagcaggat atgccggagg ccggcatgaa gaccattccg
    11521 ctgcactatc tcaccatgac cccgtatgat gataagttcc agggacttca cgtcatcttt
    11581 cccaaggaga atgttgacag aacgctcgga gaaatccttg ctgatgcagg caggaaacag
    11641 cttcgcattg ccgagaccga gaaatatgcc catgtgacct tcttcttttc cggcggaagg
    11701 gagaaggagt tcccgggtga ggaacgtatc ctggtccctt caccgaaagt ggctacctat
    11761 gacctgcagc ccgagatgag cgcttacgga gtcagggatg cagtcatcag ggaactaggc
    11821 aaaaagaagt atgacttcat ctgcctgaat tttgcaaacg gtgacatggt tggtcatacc
    11881 ggcatttatg aagctatcct gaaggctgta agaactgttg acgaatgtgc cggtgatgtt
    11941 gtgaaggctg ccacggcgaa cggctatgag gtgatcataa tttctgatca cggcaatgct
    12001 gattacgcga tcaatcatga cggatcaccc aataccgccc attcactcaa cccggttccc
    12061 ctttacctta taagcaaaag gtttaaaagc gtcagggcag gtatccttgc cgatgtggcg
    12121 cccaccattc tgactatgat ggatatacct gttcccccgg agatgactgg caaatcactt
    12181 gtgtgaggga aacgatgctg cagattgatg acaccatagt ctccctggct cttattgaga
    12241 agaagtttgt gtgtgatttg gcggcctgca ggggaagttg ttgcagatat ggtgactccg
    12301 gcgctcctct gacggcagag gaagcatcag tgctggagcg gatatggcct gacctgtggc
    12361 cctggcttcg ccctgaaggc atcagggcaa tagaaaagca gggcacaagc atcactgact
    12421 ttgatggtga actggtcaca ccgttgatct ccaacgagga atgcgcatat accatcatgg
    12481 atgatgatgt ttacaagtgc ggcatcgaag cggcttttaa cgcaggggcc gtggacttcc
    12541 gcaagcccgt gtcatgtcac ctcttccccg tgagggttaa gagctacaac actttcaggg
    12601 cagtgaacta cgaggagtgg tctatctgca aacccggtgt cgcctgcggc gcgaaacaga
    12661 atactgaact ttacatcttc cttcgcgaac ccctgataag ggcttttggc gaggagtggt
    12721 acaacaggct gcactgggcg gccggcgaat atcataaata caacgataat ggaaagcatt
    12781 aagaactaca ttgaaacaaa cagggacaga ttcctggagg aacttttcgg gctgatcagg
    12841 ataccttcgg tcagcgcttc tgaatcaaac aaggccgata tggtcagagc tgctgaatac
    12901 atccgtgatc tgatggctgc caacggagcg gacagggcag aggtctattc cacccccggc
    12961 aatccgatcg tttacggtga gaagatggtt gatccgtccc ttcccacgat acttgtctac
    13021 ggccattatg acgtaatgcc tgccgagcct cttgagaagt ggatttcgaa ccccttcgag
    13081 ccggagattc gtgacggaaa gatatgggcg cgcggcgctg atgacgacaa aggacagctc
    13141 ttcatgcacc tgaaggcgtt tgagatgatg aatgccacag gaaccctggc atgcaatgtc
    13201 aagttcatgc ttgagggtga agaggaagcc ggttcgacga gcctccccgg tttctgtacc
    13261 gaataccgcg agatgctccg ctctgacgtg atcctggtct cggataccac aatgatctca
    13321 cctgagacac cgtcaatcac atcaggattg cgcggattgt catatataca ggtagaggtg
    13381 gaggggccgg accatgacct ccattcagga atctatgggg gagcagtggt gaatccgtgc
    13441 aatgcactgt gtgatatcat cgccaggctg aaagacagcg acggcagggt gaccatcccg
    13501 ggcttctatg acagcgtaca tgttccctct gacgccgaga gagccaagat ggccgaaaga
    13561 ccgtttgatc ctgaaaagta tatgaaatcc ataggcgttg atgccctggc tgaggagaag
    13621 ggattctcaa cactggagag cacaggcatc aggcccaccc ttgatgtgaa cggcatctgg
    13681 ggcggttata ccggcgaggg ggccaagacc atcctgcccg cgaaggcctc ggcaaagatc
    13741 tcaatgagac ttgtgccata ccagaaagct gaggaaatag ctgaactggc aatcaaccac
    13801 atcaagtcac tggcgcctgc cggagtgaaa gtcagtgccg aatacctcca cggaggagag
    13861 gcttatgtga gcccgattga taccccggaa tacctggcag ccgaaaaggc ctataccgat
    13921 attttcggca agaagcccat acccataagg agcggtggca gcattccgat catctccacc
    13981 tttgaaagga tactgggggt aaagtcgatt ctgatgggat ttggcctgga gtcagatgca
    14041 attcactcac ccaatgagaa ttacccgctt gagaatctgt ttgcagggat gagggcaatc
    14101 cctctctatt tcaagtatta tgctgaactg aaaaagaaag gttgagatca taaccagcag
    14161 tccggcctgc ccgggcctga aactactgcc tgcaccccnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnga gatcataacc agcagtccgg cctgcccggg cctgaaacta
    14341 ctgcctgcac cccaggcctg acagctcacg gccccgggcc tgacagctta cggatgcctg
    14401 tgacagtgcg ggaccattca gtcgatctgg agcttcagca gttctacctt tgttgaggag
    14461 cttttcagca ccgtgaaaag attgtgtcct atcctgattt tttcctgctg gcgcgggatg
    14521 ttgccatgag caaacagtat caggcctgcc agcgtggtgt actcatcgct ctccggcagg
    14581 tcgaggtcat actcctcgtt gaggtaatct atatcaaggc gtcctgacag gacatactcc
    14641 ttttcattta tcttcttctc cacgaggtca ctggtgtcat gctcatcttc aatgtcacca
    14701 actatctcct cgaggacatc ctctatggtg atcaccccgg cagtgccgcc aaactcatca
    14761 acaacaagtg caatgctctt cttggcatca gagagcagcc tcagcaccct gttggcggtc
    14821 atggtctcag ggacgacagg cagcttgcgc atatcagtgg caatgtcagc cggcccgtgc
    14881 agtatatcct tcagctcgta ataccctacc atgttgtcaa tgttgttacg atagaggagt
    14941 atcttggagt ggcctgactc gatgaactga tgccttagct cgtcaggtga tgagtgtacc
    15001 ggcagggcca caatctctgt ccgtggcacc atgcactcgc gtgcctttac cgacgagaaa
    15061 tcaagtgcgt tctgaaaaat cctgagactg ctgatttcct cttccttctc ctcatccgtc
    15121 tcaaccatgt cattgacgaa atggtcaagg tcaatacggg tgaatatcct gttttcctgc
    15181 tcttttctct cggtttttat accgaagaaa accctcatga tgatgtttgc aatccagagt
    15241 gtgaacttgc tgaccgggta gaagagataa tagaagacag ctgtggggac agcaaacagg
    15301 cgaaggaaga aattcgggct gatccgcacc accgacttgg gaaggaagtc ggcaaacaca
    15361 agaataatca tggctgtgat taccgtctgg ataatgaaga tcagtacctc tgaggggatc
    15421 aggttttcaa accatgattg catcaggtca gcggcgagca tactgaatat gaccgttgca
    15481 atgttgaccc ctatcagcat ggttgatata tagaggcccg ggttgcgggt gaagatggca
    15541 attatacccg atccgaaatt accctgtttg cggtcaagct ctatgcgaag cttgttggag
    15601 gcaatgaatg caagctccat tgccgaaaag aatgcggcaa ggagaattgc gaaggggatt
    15661 atgacccatg ggttcatatc ccaaatttac agcttttttc gctgtgagtc acgaatccat
    15721 gccagaacaa atgctgcgac ggccatcaca taaaacagcc atgccctgcc tatgcctgcg
    15781 ttgactgtct cacggacagc tattccaagg cacatcagtc ccagaatgat ccagatgatc
    15841 tcaatcagca ggagtgactt cctgttcatc cctgatataa atagttcctg ttacgttttt
    15901 tatcgtccag ttggagaagc gtgagtctga attaaaccct gtgcccatca caatctggtc
    15961 attatgtgtg aatctgacaa acctgtcaga ccagattctg tccctgggct ggctccagaa
    16021 aagcaattcc gtctccagca cttctccctt attgctcacg gccaccacac tgtcacgaag
    16081 ctcccataca tcatccttct ccgtgtaacg ggcatagttg gaagtgatgc ttgcctgagg
    16141 tttgtctgtc cggtcataga acaacactgt cagccctttc gggaagaggg tatacggatt
    16201 ctcttccgag gagtactgct gtatcagggg tgtctgcacc accattgtta ctttgccgga
    16261 atcagaatac atggtttcta cattccttgc cgaaagtgtc ggcagcccct ttatcatcag
    16321 atcctttacc gttatttcct tcttgtcaca tgatgaaaac aaagcagtcc cggcaatcag
    16381 gactgctatc atgatattgt atgttgtgaa gggcttcact gccgttattc cttcctgaac
    16441 ctgatggttg tggattcatt gatccagccg ccaacctggt aggaagcgcc ctcctcaacg
    16501 aggctcctga aaaatgcctc ttcctttgtt gggaagagtc tggagtatat gctgatacgc
    16561 tcgtcgatct gttcctttag tgtgggatca gtgtttcttg ccctgacgag gtagtcaacc
    16621 accacccagt aaacggtctg gttctcgaat gtttcgccta tccgtgaccc ggcgtatgct
    16681 tcagcgatga tgaaaagggc gttgccgttg gtgggatcaa gtgagtatgc agctcttgca
    16741 aagtcgcgtg cagcctgcct gctgtctgac atcagctcaa gggtggccag tttggtaagc
    16801 agaactgatt tgactttgtt gtctgtctcc agttcaatgg cttcagtgta gtatttttcg
    16861 gcttcgttgt atttcttgcg gaccagattc atttcagcca gctgagctgc tgatgatgca
    16921 ctcggatcag cgcggtaaag gttggtggat gccttgtagt aaagctctga gtctttgcat
    16981 cctgtttcag tgagcagggt gaggaccttc ctgagcatgg cggtgtcacc ggggttgttg
    17041 tcaacgtcgg cagtgaagag atgctcaagg ttctcacatg tggcagctcc ggtggagcgg
    17101 aagattgcat caatgttctt cctggcttca agatatcgtg tatcaccggg ttggcgtgaa
    17161 agctggttgt caaccacctt cattgactgt gagtatgcag tgataatttc gggcgcctcg
    17221 atgagcctga gggcatagga ttttgctatg acggccatat agagactgga gtaggtatgg
    17281 tcaaagcttt cgggcgatgt ttccatcaca cccttgatga gatcatagca cttactggtg
    17341 tattcaacat catttccccc gaactcgtac agggcaaatg ccttccggag attatttgcg
    17401 ggctcttcgt tgaaatacct ggttcgctgg tcaaggatca tcaatacagt atcaaggtaa
    17461 gcgttgtcct tcgtcttgcg gaaaaactcc ttgtacatcg tttcaccctt aatataggtg
    17521 ttctttgatg atgcgggaca ctcattgaaa accagtctcc agaactgaac agccatgttg
    17581 tagtccttgt tatcatagta ggttgaatag agtgatgtgt tgcgcaggca ccttgtgctg
    17641 tcctcacctt ttccaaacgg tgtgcctgtt tcaatcccca tctgtgcctg gagggtaaat
    17701 gaaaacacag acagaagcaa tccggtcaaa atagtgctgg ttgttctttt catctctttc
    17761 ttattcattt tttaatcata ctgcctttta atgaaccagt aatcatacag attcaggctg
    17821 gctccgaccg atatcctcgt ttctcgcggt atactcccgt ttcctcccct ggttgacagg
    17881 tctaccatca gtgagatacg tgatctcgat cttttcattg gtatacctgt tccaaaagtt
    17941 atgccgtact ctttcaactc atctgctcca aaaagggtat aaccctcacc atagcggcat
    18001 cctatgcggt attcaatacg gtcaaaaaaa ctataattcg aatatctttc aggtatgtat
    18061 tctgcccctg catggagtga aagtgcatca atgtaggttc cataggtccc ggggagtgaa
    18121 gctttggtcc agcgtgtata cacaacatct gctccggctg tcagcttgtc agacttgcca
    18181 agcgagaagc ctgccctgaa tgactgtggc atcctgctgg tggttgagag ttttgcgtgg
    18241 gtaagcgtat caattgaaaa tatggaggtg gaggcggtgg agtacctcat tgtaacatcc
    18301 tcgttgttgg ttttcaggtc atatgccggc gtgtaggtca acccggcaat gatgaacctg
    18361 cccccggaaa gattgagcat gaattgtacc gatgcatcat aaccgaatcc tctcatacct
    18421 gatgtgccct gtttcctggt attgaaatga ttgttgtcag cagtgaacgc aaagtcattg
    18481 atcctggttt cctccccgaa catgtagaag atatttgctc ctgccgagac aaaaggaagc
    18541 agccggagcc cggtgccaaa aagagctttc tggaatcctc cgctgccgtt atgcagttcg
    18601 taaagggagc cggtcactcc ggagccttcg gaacggtatc cgatactgta tgatccgttg
    18661 gcataaggca ttaccgcggc agcaacgccc cagcccttca ttatcggaaa acccaacatg
    18721 agatgggcga atgtaatgtc ctgggaataa tatctgaggc cgtctccttt aagctttgcc
    18781 atggcgtaat caataccgaa gtcgaaaagg aaggaggttg tgtcagtgct gctgtacgag
    18841 gcaggtgtca ggtagttcag cgtgaggttg tccctgatac cgctgctgat gccccccata
    18901 gccagcgttc tggaatttcc ctgaagctcc aggttgccaa ttccgtacct cgcgaaaggt
    18961 gtattgactt ttttctgacc tgcaactcct gtagccagga gagcaaggag acttatcata
    19021 attgctctta tgctcttata catttgtttc cagtaaaaaa ttcaatccct ccgttaccag
    19081 gtcagggtag tgaagtagtc tgcctcctgc cagcggtgtg accaggccgc tgtctccgcc
    19141 cgtaatgacc gcaacggaat tatggtattt ttccttaaag gtgcgaatat actcaattat
    19201 ttcatacacc agacctgtta taacaccgcc agcgatagca tcttccgttg tttttcccgg
    19261 gacagtgaac ccggttctct ttgtcaccag tggcaatttc ccggtgaatt catgaagtgc
    19321 cctgaaccgc attgccaaac ccggcgagat gctgccgcct ctgtaaacac caccggtcac
    19381 ctggtcaact gtcagtgcgc tgccggcatc aatcaccagc aggtcagccc cgggatgatg
    19441 attaacagcc cctgctgcgg cagccagacg gtcagtgccg agagtctccg gtgtcatata
    19501 gtctattgca aacgggtata gtgaccgtga ggtgaggtga tgtacacgcc cggccctggt
    19561 gcgaagccag tcaaaaagct ccgactggtc accgctgaca ctcgagacaa ttgctcctga
    19621 cagcctgcgg cggccagtga acatgccgag catctccgga tcagcactgt caaaccgctc
    19681 ctggcttatg atcacaccct cgctgacaag cgctgccttg actgaggtgt ttccgatgtc
    19741 aattatcagg ttcatcagtt aatttgcttt tacagcctga gccgactcat gccatgcgag
    19801 tatctctgtg aaaaggtcaa tagcctgtac cacagtggtg acactcctga ccgtcaggct
    19861 cagcttgttg ttgttctgtt tcactgacat gttgctctgc ctcctgttga catagttcat
    19921 caccgacacg aatagtggcg agcgatagaa ctgcgacgac tggtccgtca caaagttagc
    19981 aatcagtgtg ttatttttca gaattacttt ttcaattcct gtccgggctg ctgcccattt
    20041 aagcctgacg agctgcagca gcgccagcgc aggcggagga ggcagcccga acctgtcagt
    20101 aaggtgcgac tcataggctt taagccctgc ctcatccctg atctcattga gctccttgta
    20161 gagccttata cgttcgggta tgttggagac gtaagcgtca gggaacatta tctcgagatc
    20221 ggtctctacc gtcacatcct caacaaattg tttctctttt tctccggcag cgtgctccct
    20281 cttctctgcc ggtgcggtga aaaggtctcc gaactcttca tccttgagct ctctgacagc
    20341 ctcgtcaagg atcttctggt aggtatcata accgacatcg gctataaagc cgctctgttc
    20401 acctccgagg aggttgcctg caccgcggat gtcaaggtcc tgcagtgcga tattgaatcc
    20461 gctgccgagc tcatagttct cctctatggc cttgagcctg cgcctggcgt caccggtcag
    20521 taccgaaagc ggtggagtga gcaggtaaca gaaggctctc ctgttggagc ggcccaccct
    20581 gccgcgaagc tgatgaagct cagaaagacc gaaactatgt gcatcattga tgaagatggt
    20641 attgacattg gggatgtcga gacctgattc gataatggtt gtagccagca gtacgtcata
    20701 atcaccatgg atgaaatcag acatgacatt ttctatctcc tcaccctcca tgcggccatg
    20761 taccactgca atctttatct ccggacagaa gcggagcagg gagtctttca cctccataat
    20821 gttttctatc ctgttgtgga tgaagaaaac ctgcccgtta cggctcacct cataaactat
    20881 cccctcgcgg atgatatcct cattgaagcc gtgaagttcg gtgatgatgg gcatcctgtt
    20941 gggtggaggg gtgttgatca ctgacaggtc acgggctccc atcagtgaaa actgcagtgt
    21001 ccggggtatg ggggtggcgg taagggtgag ggtgtcaaca cctgtcctga gcgttttgag
    21061 cttctctttc gcggctacgc cgaagcgctg ctcctcatcg atgatgagca gacccaggtc
    21121 cttgaacttc acctctttgc cggtgagctt gtgggttccc acgatgatat caatttcgcc
    21181 gtcggcgagt tgtttgagga ttttcttctg ttccgcaggt ttacggtgcc tgctgatgta
    21241 atcaaccttg caggggaagt tctcaagccg tgatgaaaag gttctgaaat gctgcagcgc
    21301 gagtatggtt gttggtacca gcactgccgt ctgcttcccg tcagtggctg ccttgaatgc
    21361 tgccctgatt gctatctcgg tcttgccgaa tcccacatca ccgcatatga gccggtccat
    21421 cggcaccggg gtctccatat cctccttcac tgccatggtg ccggcaagct gatccggcgt
    21481 atcctcccag atgaatgagg cttccagttc gcgctgcagg tatgagtcgg gcgagtaggc
    21541 gaaaccgccg gaggcttttc gctttgcgta aaggagaata aggtcgcggg ctatatcctt
    21601 taccttcttc ttcgtagcct gttttaccct ctgccatgcc cctgtgccca gcttgttgat
    21661 gcgcggcggg gtgtcatccc tgcctttgta tctggagatg cggtgcaggg cgtggatgcc
    21721 aacatacaat atgtcattgt ccttgaatac cagcttaatg gcttcctgca tcctgccgtt
    21781 gacctcaatc ttctcaagcc cgccgaaccg gcctatgcca tggtctgtat gtacaacata
    21841 atcacccggg ttgagatcag taagctctct cacggtcatg ctgtcacgac gtgtgaagaa
    21901 gcccctgatc cgaaacttgt gataacggtc aaagatctga tgatcagtgt aaacggctat
    21961 cttaaggtca tggtctgtaa agccctggtg caggttgagc aggagtggag tgaactgaac
    22021 tgccggatca ctttcttcga agatctcctt caggcgctgt ttctggacct cgctctctga
    22081 gatgataaag ttggtgtagc catcctgaac gtttgaggcc agctttgatg ccagcaggcc
    22141 gaagttcttg ttgaatgccg gctgcggctc ggtgcggaac gcgatattga ccgtatcagg
    22201 gttgtgcctc cccctgccga agaagatacg ccggtgacgc gagcaaagtt cttcaaggtg
    22261 acggcccgtg atgataatgt cactacggtc ggatatctct cccttcaggc tgcggctgca
    22321 ggcctgctcc catatattgt cagtcttctc aacgatgtag tcaaaactat cgctccagat
    22381 gattgatgac ggaggaatga aatcaatgaa gctgtcagcg ttctcctgga aggcaagcag
    22441 ctggatgtca ggaatgacct gtatctcgtc atggagctgc agggagagct ggtcatcagg
    22501 actgaacgaa cggattgagt caacctcctc gccgaaaaag tctatcctgt acggcttgtc
    22561 tgtggagtag gaaaaaaggt cggtgatgct tcccctgatt gcgtactgtc ccggctcata
    22621 gacaaaatca gtgcgctgga agttatattc acggaggacc tcctcgagga actcgattga
    22681 gagttttgcc ccttttttta tgaagaaggt gttttttttg aggcttgttt tgctgatgac
    22741 cttctccatg accgattccg ggtatgagac gatgatgcat ttcctccgcc ccgaactgag
    22801 gaaattgagc acctcagccc tgagaatgat gtttgccggc tcagtctgct catacatcac
    22861 tgaccgtttg taggttcccg ggaagaagaa gatgttgtta tcacccatca gtgatgtgag
    22921 gtcattgtag aagtaggcgg cctcttcctt ttcgggcagc agcaccacgt gtgtggttgg
    22981 ttgctgtgat agtgcgccgg ccatcacgat ggagcgtgat gagcccgtca gcccgtgtat
    23041 agtcaccggc tctgcgctgt gggcggccag catctcctca agcctgcgga atgatggatg
    23101 ccgcctgtaa atctccctga aaacgttgcc ggccattgat tttgtctgag tgtgcaaaat
    23161 taacaaagct gctgctcacc gcaaagagaa tgagattctc tttcagtgga aatcactcat
    23221 gacagttctg tagggggcag tataccatgt gggaggcgcg tgcctcatat acatcccggt
    23281 gccggttcgg gcatcccgct cgcgcatccc gcaacatccc attcactggc acatgcnnnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgg ttcgggcatc ccgctcgcgc
    23461 atcccgcaac atcccattca ctggcacatg cccacaagtg tcctgatcat gtcaggtgat
    23521 ccgcagtaga atggcgtccg ctgatggagg ctttcgggct tcagcgaaag gattctcccc
    23581 gtgccgtcag tggtaacgcc gccggcctgt tcgataataa acgagatggg attgcattca
    23641 taaatcagcc tgagcttccc ggaggggtag gtgattgaag gcggataaag gaatatgccc
    23701 cctttcagca tgttgcgatg cacatcggaa accagtgacc ctatatacct tgagaggtat
    23761 ggccggctgg tgtcaggatc gttctcctgg caatatttga tatatttctt tacccaatct
    23821 ggaaacttga tatagtgtcc ttcattgatt gaataaatct ttcctgtttc cggcgtcctc
    23881 atgtttgggt gggaaagaca gaactccccg atggtagggt caagggtaaa gccattgact
    23941 ccgttgccgg tggtatagac cagcatggtg gatgatccgt agataacata gccggcagca
    24001 acctgcttgc ttccttcctg aaggaaatcc tcaattgaaa ccttctcatc gcgttcagtg
    24061 atacggcggt aaatggaaaa gattgatccg accgatacgt ttacgtcaat gtttgatgag
    24121 ccgtcaagcg ggtcgataca gaccacatac ttgccccggg aagcaaactc accggtgaag
    24181 ccgacctcgt tctggttctc ctctgaggcg atacccgcac actgacctga cgctttaagg
    24241 gcatcgatga atacctcatc agcataacgg tcaagcttct gctgttcctc accctgcggg
    24301 ttttcaattc cgaccctgcc cagtatctct accagtccgg ctttgcggat cttggcgttg
    24361 accatcttgg cagcagtgcc gatgtgatga agaaggctcg aaagctctcc ctgggcaaat
    24421 ggaaagtctt tctgtttgct gatgataaag tcattcaggc tggtgatatc ataacttggc
    24481 atactaaaaa gagttttggt tagtcgtttg tcagttcagg tcagcccgga aagctgccct
    24541 gtatgcgtaa accttaccca cattgttgta gagttcataa ccgaaagtga ctgattttgt
    24601 ccttttctca acccggtttt cttccggaaa atagtaccag tcttctgtaa atgagatctt
    24661 cccgatcttt gttctgtcgt ttttaaactc accctcaatc ttttttacat cagcaggctt
    24721 gagcggctcg ccgctgtgat agtcatacac tgtcatggtg ccgtcataga tactgctgaa
    24781 gataccgtca acaagtgacc ctccgttgta gcctgccacc ttttccactt cccacgggtc
    24841 accttc
//
LOCUS       DAOPBK010000010        24187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2229_length_24187_cov_5.946585, whole genome shotgun sequence.
ACCESSION   DAOPBK010000010 DAOPBK010000000
VERSION     DAOPBK010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2229_length_24187_cov_5.946585"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3848
                     /locus_tag="PLA17_01315"
     CDS             <1..3848
                     /locus_tag="PLA17_01315"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPK84081.1"
                     /translation="DACNNYSEVTQTIYVADTIPPTFTPPADITICRNINGEYNRDVT
                     NTGDATGEEDNCDTSLDAAYTDSDVNMGTADAVGFITRTWTLTDNCGNVTSHDQIIWV
                     QPVPRIAVTVPDTIFCNGSTVEFAIDSLVVSRGEVMYDLDVTYPAGLSGTLANGQNQI
                     ANISDLLTNSSDSYQTVTYRFRPYIQGKTGDPTCHDGVEVIITVHIEPTARVALTLAD
                     DELCNGENVNIQIATPTIAYNGLEFNVEAINSHPEITGCTDRTGLVVSDMITEALNNS
                     GDTARLVTYVVVPATLDVSGNQKCYGIRDTVSVWVNPTPRATPVNYVPHICYGDTTDV
                     LLLSPTVMRSGINEFNYSITSTASTDVVAGNRTALNSVVPGTHLKFPYTNESDTIQSV
                     FFHIIPKVTGPGCPYGPPAISEIKVHPHPLQSLEILDSITCDGGQDGALQVICARGYD
                     SLWVAWTGPDYWEEAAYNMFIAEECSQGYYTATVTDSLGCSSSDALNFLAPNTEVSLY
                     FGQFISCPGAEDARMVVALTEGHAPPHYYNLVWNGTDTVSAGWMPPEGHFFTIDNLKP
                     GDYMLSVVDANGCREELPRTLYDAPVTFANFEKSIYADYNISCKSYSDGTIRVSEIYS
                     YYMDGTDTVFVSSRAPYSYQWSASDGGVITGSSTDSLLVNIPAGTYTLVVTDSQGCIF
                     TFTETMTEPDGIDLIAEDVSLSNDGNYQISCHGASDGYINLQFDGGAGAYSYAWTGPD
                     CFTANTASLTGIRAGTYNLTVTDGNMCHRYYTYILNEPDSLSIAVSTSHTPDNEFNIN
                     CNGGDGTIDITVTGGSGEGTYSYQWTKSGDESWSSTLEDLTVKAGTYQLQVTDVNGCV
                     NTRSVEMTEPDALNLSLAVSDITCLNAPLYNDGSIDLTVSGGRPPYSYSWTGPEGFTS
                     SLEDISGLTEGTYTITVTDAYGCSIASDTLLSLPEPISLESRVSDYNGFSVSCSGKSD
                     GWLRIIPQTGTAPYSYSWSGPAGFTATTDSIYSLREGTYSVTVTDKHLCSVTHDITLV
                     SPGPLSMTLDIGLSNGGSYNINCYGAATGRVNITAVNAVGTPEYIWSDGLTGAARDNF
                     AAGYHEVIMTDANGCVADTSLTLTEPDSMRINFSLISPFCPESNDGSIFAGVTGGEGA
                     YTFTWSDGQTTQEAVGLVQGLYTVEARDFNGCVVSDSMSLKALNDICVGIPNAFSPDG
                     DGINEFWNITRISYYSEVEVIILNRWGEIVWKSEKGYPEPWDGRASDGKVLPMDSYHY
                     AIDLHNGEKPIVGHVTIIR"
     gene            3879..4856
                     /locus_tag="PLA17_01320"
     CDS             3879..4856
                     /locus_tag="PLA17_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPK84082.1"
                     /translation="MKRGLIILAVIMMASGLDAQQLPIYSQYLFNKYLINPAVAGSDG
                     YTSINLTTRQQWTGYQGAPQTYSLSLNGRLLKQKYLIRQNLFDRKVFRPRSEGRVGIG
                     GTVFSDINGLVRRTGFSTSYAYHIWLQGGTQLSFGLAFTGYHYKIDQRQIQFEDPDEP
                     WLNTDFRKGIFVPDFNFGAYLLNRKYAIGFSAQELMEGFIRAGSQAYRDLKVQRTYYL
                     FGNYDFEPYIKTVIQPSLLIKMSEQMRPQADIGITYSYDNKLWIGATFRTGSAIIANV
                     GVRRDKLFFGYSFDFTLNSIQMSTYGSHELIVAARFGDTTRKYRWLDRY"
     gene            complement(4853..5749)
                     /locus_tag="PLA17_01325"
     CDS             complement(4853..5749)
                     /locus_tag="PLA17_01325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK84083.1"
                     /translation="MAEQLVCPACGSSDSAGLFHAKDHLVSNSEFLVRRCNDCGMGWT
                     ADPPPEEEAGNYYLSEEYISHSDRKQSIADYIYHLARNFMLSRKRRLVVSAVGKKTGS
                     LVDIGSGTGYFAAFMKKRGWQVTGIEISDNAREYSVARFAIRAVTPAEIREMPPGSAD
                     CITFWHVLEHLYDPDMWMGEVNRILRDDGRCIIALPNFASADSTWFRNRWAALDVPRH
                     LWHFTPAAFVRFAGRHELACEKISALPLDLFYISCLSYRNEGKSIPLIRGVATGLFIA
                     LRNLFRKEQASSLVYVVSKKKP"
     gene            complement(5749..8205)
                     /locus_tag="PLA17_01330"
     CDS             complement(5749..8205)
                     /locus_tag="PLA17_01330"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPK84084.1"
                     /translation="MKPNSFKSALPHIIAVLLFTVISFAYFFPVLEGKKINAHDTKVY
                     EGSSKEISDYREKHGGEPLWTNSMFGGMPAYTISMKYPGNLFKHLDNFLKAFKTPVAA
                     LFLSMLGFYIMLLLFGVNPWLAIAGAIAYGFSSYLFVCLSAGHNTKAYAMAYMAPVIG
                     SVVYSFRRNLLTGASLFALFLSLQIMANHVQITYYTGIIVLVFGIFELVHAVKSRMIP
                     SFLKSLGVLVVAALIAFSVNFASLYSTWEYSKESTRGKSELTTAAGTSEKGLDPEYIT
                     QWSYGIDESMTFLIPNFRGGATAPFDSNSETVKALRKNNMGQAQGQLYRYWGKQPSTS
                     GPVYFGAVVLFLFVLGLMLIKGREKWWILTAALLSLFLSWGKNLMPLTTLFIDFFPGY
                     NKFRAVSMTLVMAGVLVPLLGVLALREISEGGVNREKAARSVHIAAMITGGLAFIFFL
                     IPGLAGSFLRPDEMALPDSVAWIKDAMAADRKMMIRTDALRSSLFIAGAAAIIWFYLK
                     NKLKPAYALLGMTALFLIDQVPVDARFLGKSSFETKRASANSFAPSEADKIIMGDEDE
                     FRVLNLTVSVFNDASTSYHHHSIGGYSGAKMKRYQELIETSLSDDINSLITNLRTATT
                     MEEAEGALGSLGILNMLNTRYIILDPGSAPLVNRHAAGNAWLVNRVTLAENADAELLA
                     VKTINPKEEAVVDRRFAELIGVDETAGTPGDTIFLTSYEPNLLTYKAELSSERVAIFS
                     EIYYKYGWKASVDDKPADHFRANYVLRGMTLPAGSHTVTFMFDPESYRIGNRVSLAGS
                     VVLLAFLLLAAFPLLKKRNG"
     gene            8322..9803
                     /locus_tag="PLA17_01335"
     CDS             8322..9803
                     /locus_tag="PLA17_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPK84085.1"
                     /translation="MGIVQRQTIRGTAWSYLGALLGFVNIILLSPRIFTTGEIGVVQL
                     LLSFATMLAQFSSLGFTNVINRLFPYFRGTRGRHHGFLGLAVVITLAGFIIALIFLKF
                     YAPHFERVNLERSPLISRYSIYLPALLLVTLLFNLLDNYNKVLYDAVLGTFLREFFFR
                     VLNLGLIVLFWAEIIDFDGYVFWYVVSQGVLLIIIFISLLMRGEISLRLEPGFITPHL
                     RREIILLSLFGILTGISTVTLTTLDKLFINTYLGEAEVGIYSIASYFAVMITLPGRSV
                     SKISIPFLAESWKKNDLEKIDDMYVRSSVSQYAVGLLIFIGLLVNIDNIFRLLPDIYS
                     KSAAVIVLISFGNLVSGSAGVNGVVLSTSPLYRYQTWLMFILIGLFVGTSLIFIPLFG
                     ITGAALASMISNIIYSMLGVLLTGTRFGLWPYSMVHLKMTLVALAALAAGYFLPQMPL
                     ITDIIVRSLLVTMIYTAGVLFWRLSDDLNGIAESFVERFRRRR"
     gene            complement(9873..11156)
                     /locus_tag="PLA17_01340"
     CDS             complement(9873..11156)
                     /locus_tag="PLA17_01340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK84086.1"
                     /translation="MRAVPAKVLIITYYWPPSGGAGVQRWLKFTKYLPSSGWFPEVLT
                     VDPLYAAYPFRDDSLYGEVPVDVKVHRTKALNFFALYNRDQSKIPHAGFASGKDKGFK
                     STVSRFIRGNFFIPDPRRGWNRFAFRRASELIRSQNITHIITTSPPHSTQLIGLRLKR
                     RFPGLKWVADLRDPWTDIYYYDMFRPSLPARMLDRNLEKRVLSRADRIITVGKTLASL
                     LAAKTEAVAGKTEILPNGFDEEDFQGIVSSVPERFTITYVGTLSPAYPVRGFIDAVKD
                     MRINGKQVALQFVGTVPEEIRALFPAGDEGDGPRFISYCEHPEAIRYMMSSSLLLLII
                     PDHSSNRSILTGKIFEYLATEKPILFLGPEDGDAARLLTLCGYQGIFGYDDVEGIREF
                     ILRVAEGKPVKRSGYHLEYSRRALAVKLGNILDRL"
     gene            complement(11158..12708)
                     /locus_tag="PLA17_01345"
     CDS             complement(11158..12708)
                     /locus_tag="PLA17_01345"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPK84087.1"
                     /translation="MAQEDVFRKLVAHCKEYGYVFPSSEIYDGLGAVYDYGQYGVELK
                     NNIKKYWWDSMVLLNENIVGLDSAIFMHPAIWKASGHVDAFNDPLIDNRDSKKRYRAD
                     MLIEEHLEKLSEKIEKEVVKARGRFGEKFDEALYRSTNPRILETREKYDALHKRFVEA
                     MAGPDLAELRQIIVDNGIVCPVSGTCNWTEVRQFNLMFATETGPTSDGSMKIYLRPET
                     AQGIFVNFLNVQKTGRMRIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQFFVRPG
                     TELDWFSHWKEARMKWHRALGFGDDNYRFHDHDKLAHYANAAADIEYRFPFGFREVEG
                     IHSRTDFDLRQHQQQSGKKMQYFDPEINESYIPYVIETSIGVDRMFLQVIAASYFEED
                     LTKEDGTPDSRVVLRLPAALAPVKLAVMPLVRKDGLPDISRRIISDLKFDFNCQYDEK
                     DSIGKRYRRQDAIGTPYCITIDYQTMQDETVTIRYRDSMEQERVSVSQLRAIIADKVS
                     MQEQLKKL"
     gene            12831..14408
                     /gene="nadB"
                     /locus_tag="PLA17_01350"
     CDS             12831..14408
                     /gene="nadB"
                     /locus_tag="PLA17_01350"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPK84088.1"
                     /translation="MKIRCDYLVIGSGIAGLSFALKAAAHGEVCVITKAKMDDCSTNY
                     AQGGIAAVMYEPDNFEKHVRDTMIAGAGYSNEEVVRMVVSEAPERIRELIEMGVDFDK
                     KPDGTLDMAMEGGHTEHRILHHKDYTGAAIEKVLIKKAREHKNIRIYEDHFAIDLLTQ
                     HNLGEYVTREWKNIECYGAYVADLTSRRVITFLARVTVLATGGCGNIYLTTTNPPVAT
                     GDGVAMVHRANGVLENMEFIQFHPTSLFNPRERPSFLITEALRGFGAELRNRAGEKFM
                     PKYHAQGSLAPRDVVARAIDNEMKLKGDDFEWLDCTHLDADALRSHFPHVYEKCLSEG
                     IDITRDMIPVVPAAHYSCGGVRVDRNGRSSIDRLYAIGEVSSTGLHGANRLASNSLIE
                     AAVYAHRAARDASARIGSLTHEEKIPVWNYHGTTHLEEMILVTQNYREMQMIMSNYVG
                     IVRSDLRLARAKARLEIIYQETEDLYRRSTISRELIELRNLIAAGYLVIKMAQNRQES
                     LGLHYSIDYPARPGSEF"
     gene            14420..15424
                     /locus_tag="PLA17_01355"
     CDS             14420..15424
                     /locus_tag="PLA17_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPK84089.1"
                     /translation="MEVNETIDTPEAGGESPFISAAFTGKNSFWRYFTGVIAPFLAAI
                     IISGLLLKAVIIASSPDGYAMLQKDTMPDFEAMGINLNLGFVLNVFPFLIGLLAFILL
                     VRPLHNRTFGSVINGGRKVRWGRMMFAALVWIAVSALYLLYALKSDPGNFRINNTSNS
                     LIVLALLALLLIPFQAAFEEILFRGYLMQGFAVLARNRWIPILSTSILFGLMHGFNPE
                     VSKYGFLTMMPQYIFFGLLFAVLTMFDDGIELAIGAHAANNIFLTVLVTHEDMALQTP
                     ALYEQIQIYPWEEFGGLILQSLIFIAILALVFKWKDIRKLYARIIVPAAEVSDAVDAG
                     "
     gene            complement(15401..16414)
                     /gene="dusB"
                     /locus_tag="PLA17_01360"
     CDS             complement(15401..16414)
                     /gene="dusB"
                     /locus_tag="PLA17_01360"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPK84090.1"
                     /translation="MKLGNTILREQPLFLAPMEDITDPSFRYVCKMFGADFMYTEFIS
                     SDGLIRDGRKSVKKLDLFDYERPIGIQLYGHITESMVDAALRAEEANPDLIDLNFGCP
                     VRKIAARGAGAGMLQNIPLMTEMTSAIIRAVKTPVTVKTRLGWDENSKIIVDVAERLQ
                     DTGIAGLTIHGRTRSQLYRGEADWTLIGEVKKNPRMKIPVIGNGDINGPERAKEMFER
                     YGVDGIMIGRATFGRPWIFREIRHYLDTGEILPEPTVREKADLALLHLDKSLEHKEGK
                     PAILEMRRHFSNYFKGLHGFKETRLKLLTSTDVDEIRSIIGEIKEKWGDFTYERSTGI
                     YGI"
     gene            complement(16422..17096)
                     /locus_tag="PLA17_01365"
     CDS             complement(16422..17096)
                     /locus_tag="PLA17_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84091.1"
                     /translation="MSKEIPPFRLAFSMKTLVRIQAFLPLFENSISHQRQTLVNQNEK
                     AKSNQETARKARLYLTHFLRVMNMAVTRGELPPETRTFYGIASDDSTLPSLATENELV
                     AWGKRIIDGEEYRIRKGYSPVTNPTIAVVKVRYEKFLETRSHCKTVNRRASDCVNKTA
                     GMRQEADELITLLWNEIEASFSSLPEEEKRANAEKYGLVYVFRKHEKTRKNLSLTLFS
                     ANQENQ"
     gene            17312..18697
                     /locus_tag="PLA17_01370"
     CDS             17312..18697
                     /locus_tag="PLA17_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPK84092.1"
                     /translation="MFEKEVYIKRRQRLKSLVNDGIALFIGNVEAPMNYPDNTYHWRQ
                     DSDFLYFFGIDLPGLAGVIDFNSGDEIIFGNDFTVDDIVWMGPQPSIAELASRAGVVL
                     THPMNKLDEMVHDALKAGRPIHFLPPYRAETKMTLGALLKENPCQMRTKASEKLIKAV
                     VSLRSIKEDIEIVEIEKAIDITCEMETTAMRMCKPGVTEQEIFGVLQGIAYSRGAGPS
                     FPIILSINGQTLHNHVHINVLREGRMMVTDCGAESNMHYAADLTRTTPVGGKFSQRQK
                     EIYEIVLRANTESIKNAGPGISNRDLHLGAWRIIAEGMKDMGFMKGDIDEAVAAGAPA
                     LFMPHGLGHMMGLDVHDMESLGEDYVGYNDEVKRSNQFGLRSLRFALPYKPGHVFTVE
                     PGIYFIPELVAKWKAEGINEAFINFAKVESYLDFGGIRIEDDVLITPTGSRLLGKPAP
                     KTVEEIERTCI"
     gene            18865..19665
                     /locus_tag="PLA17_01375"
     CDS             18865..19665
                     /locus_tag="PLA17_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPK84093.1"
                     /translation="MKRNIQFEGVRIYYTVRGKGRPIVLLHGYLEGGEVWDPLAEKLE
                     EDYRIISPDLPGHGESGVKGEVHTMEFLASAVREVIRDAGEKRVVMVGHSLGGYVTLA
                     YVELYPEMLSGYVLFHSHPHADTPEAIARRKREIAIVRAGKKNIMYPGNVSMMFAKEN
                     LKSMHAQLERSRQIASRNSVEGIIAILNGMIVRPSRQYILEKGEVPLLWILGRHDLYF
                     SPGKAQYDIGLPLNARVVILENSGHLGFIEETEKSALLLKDFAGRLTW"
     gene            complement(19673..21265)
                     /locus_tag="PLA17_01380"
     CDS             complement(19673..21265)
                     /locus_tag="PLA17_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPK84094.1"
                     /translation="MKYNKAYLPLIIGISIAIGILIGFYMPHPQAGFYTNFPPSNDKL
                     NRILDIIESDYVDTVNRNALIEEAIPVMLRKLDPHSVYIPATDLAQANEPLQGNFDGI
                     GVSFNMLTDTVLIISTISGGPSEKAGIIPGDKIIMVNDSLIAGRNIPDEDVVKMLKGP
                     RGTRVDLKIERKGAKELLPFTIIRDKIPLYSVDVSYMMSDDVGYIKISSFALTTHDEF
                     KKGLNELKETGMKKLILDLRGNSGGVMDAAIQIADEFLPEGKLIVFTMGKSTPRQEMK
                     ATAKGEFETGELVVLIDEWSASSSEILAGAVQDNDRGTIIGRRSFGKGLVQEPVMFRD
                     GSGMRLTIARYYTPTGRSIQKPYDQGVDKYYEDLGYRLMHGEFEEADSVRLDDSLRFT
                     TPGGKVVYGGGGIMPDIFIPIDTAGISEYFLAVRNSGLLYRYALKFTEDNRETLKKYN
                     ELPELIRWLEGQNLLSKFVGYAGQNGVPARKDQIETSKNIIEVQLKAYIARNMLDNKG
                     FYPIWQEIDKTLLEAFSYLENN"
     gene            complement(21255..21722)
                     /locus_tag="PLA17_01385"
     CDS             complement(21255..21722)
                     /locus_tag="PLA17_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPK84095.1"
                     /translation="MIKKDAIHPYGEKQEQFDRRYLRMASIWSENSYCERRKVGALIV
                     KDKMIISDGYNGTPAGFENICEDENNVTKPYVLHAEANAITKVARSNNSSENATLYIT
                     DSPCMECAKLIIQSGIRRVVYSNQYRNIDGIELLKRAGIELLYIDLGERKNEI"
     gene            complement(21756..22061)
                     /locus_tag="PLA17_01390"
     CDS             complement(21756..22061)
                     /locus_tag="PLA17_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HPK84096.1"
                     /translation="MKSIQIYQVDAFTHVPITGNPAAVCVLTDPVTEETMQKTAMENH
                     LPETAFITRGKDEWLIRWFTPMQEVDLCGHATLNGDRNESQAQHRCILKASSLSDLD"
     gene            22145..22837
                     /locus_tag="PLA17_01395"
     CDS             22145..22837
                     /locus_tag="PLA17_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK84097.1"
                     /translation="MNRIHNTQNGTTITRLTPAGCNIWVVTRNDRTIIVDTGISFDRS
                     RIERALKRKGIIPEAVILTHTHFDHAGNAAWLQAEYGSGVVVQEAESGMLAAGDTPIP
                     EGTYTFTRGMIGIGRKMQPVFRYDPCVAEHVFNDQFDLNRFGINGYVMHTPGHSPGGA
                     TVVIDDETVIVGDLVVGTMPGSPFPPFADDVTLLLNSWKKIIDTGYRSILPGHGRPIS
                     REELIKEYDKRK"
     gene            complement(22997..>24187)
                     /locus_tag="PLA17_01400"
     CDS             complement(22997..>24187)
                     /locus_tag="PLA17_01400"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPK84098.1"
                     /translation="IMENRPRIASVDIMRGLTLLLMLFVNDLNMKVAPAWLGHMEADF
                     DGMGLADWVFPGFLFIVGMAIPFAISGRIKRNEPVSGILLHIAIRTVSLMVIGILMLN
                     SGRVNPEATGMNRNLWALLMYISVFLVWNDYPKGKHKLLFTILRAAGVATLIFLAIIF
                     RSGSADDPGWMITGWWGILGLIGWGYLTASLIYVAFRDSVAWTMAAVIFFLMINILDQ
                     SGVLGFLDPVRPVLGILIQGNVPLIVLTGMFAGILIRKLKKSGDEKIILMLIVMGIIS
                     LALGFFLRKWFIISKLLATPSWGMICSGISFLVFAAIYWLADVRGLTGWTDFVRPAGR
                     YSLTTYLAPNILYHAIWMSSVPVLIYKQSSEPLVVILGSVAWALLMSGLTAILARMHI
                     KLRL"
BASE COUNT         5915 a         5919 c         6161 g         6192 t
ORIGIN      
        1 ctgatgcctg taataactac agtgaggtta ctcagactat ctacgttgca gacactatac
       61 ctccgacatt caccccgccc gcggatatta ccatctgccg taatatcaat ggagaatata
      121 acagggatgt caccaacacg ggtgatgcta ccggagaaga ggacaactgc gacacctcac
      181 ttgatgcggc atatactgac tcggatgtaa atatgggtac agcagatgcc gttggattta
      241 ttaccagaac atggactctc accgataatt gtggaaatgt cacaagccat gatcagataa
      301 tctgggtcca acccgtaccg cgcatagccg ttacggtccc tgatacaatc ttctgcaatg
      361 gctcaaccgt tgagtttgca atagactcac tggtggtttc acggggcgag gtgatgtatg
      421 acctggatgt aacctatccg gcaggcctca gcggcaccct cgccaacgga cagaaccaga
      481 tagcaaatat ttctgacctg ctcaccaaca gctctgacag ctaccagaca gtaacctaca
      541 gattcagacc ttatatacag ggcaaaaccg gtgatccgac ctgccatgat ggagttgagg
      601 taataatcac tgtccatatt gaacctactg ccagggttgc cctgacgctg gcagacgatg
      661 agctgtgcaa tggtgaaaac gtgaacatcc agatcgccac accaacaatc gcatacaatg
      721 gtttggaatt caatgtggaa gctattaaca gccatcctga gataaccgga tgcaccgaca
      781 ggacagggct tgttgtcagc gatatgatta cagaggcgtt gaacaacagc ggtgacacag
      841 ccaggcttgt cacctatgtt gtagtccctg caacacttga tgtcagtggc aatcagaagt
      901 gctacggcat cagggatacg gtcagtgtat gggtcaatcc gactcccagg gcgaccccgg
      961 tgaactatgt tccgcatata tgttacggtg acacaactga cgttttgctt ttgtcaccca
     1021 cggtaatgcg ctccggaata aatgaattca actattcgat cacttccaca gcctccaccg
     1081 atgttgtcgc aggcaacaga actgcattga acagcgtagt acccgggact catctgaagt
     1141 tcccctacac caatgagagc gatacaatac agtcggtctt cttccatatt atacccaagg
     1201 tgacaggtcc gggttgtcct tacgggcctc cggcaatatc cgagataaag gttcaccctc
     1261 atccgcttca atcacttgag atactggact ccatcacttg tgatggtggt caggacggag
     1321 ccctgcaggt aatttgcgca cgtggatatg attcactgtg ggtggcatgg acagggcctg
     1381 attactggga agaggctgca tacaacatgt tcattgcaga ggagtgcagc cagggttatt
     1441 atacggcaac agtcactgac agtctcggtt gctccagctc tgacgcactt aatttcctgg
     1501 ctccaaatac agaggtatcg ctctatttcg gacagtttat ctcatgtccc ggagctgaag
     1561 atgccaggat ggtagttgca ctgacagaag ggcatgcacc tccgcactac tacaaccttg
     1621 tctggaacgg cactgatacc gtgtccgcgg gatggatgcc gcccgagggc catttcttca
     1681 ccattgacaa cctgaaaccg ggtgattata tgctttccgt tgtggatgcc aacggatgca
     1741 gggaggaact gccgcgaaca ctgtacgatg cacctgtcac ctttgccaac tttgaaaagt
     1801 cgatatatgc tgactataat atatcatgca agagctacag tgacggaacc atcagggtaa
     1861 gtgaaatata cagctattat atggatggca ccgatactgt atttgtaagc agcagggctc
     1921 catatagtta ccagtggtct gcttctgacg ggggagttat aaccggcagc tcaacagaca
     1981 gcctgctggt caatatcccg gccggtacat atactctagt tgtaacagac agccagggct
     2041 gcattttcac ttttacggag acaatgaccg agccagacgg cattgacctt attgcagaag
     2101 atgtctcact gagcaatgac ggcaattacc agatatcgtg ccatggtgcc agcgacggat
     2161 atatcaacct gcagtttgac ggcggagcag gagcttacag ttatgcatgg acaggtcctg
     2221 actgctttac ggcaaacacc gcgtcactga cagggataag ggcaggaacg tataatctga
     2281 cagttactga tggcaacatg tgtcaccggt attataccta cattcttaac gaacctgatt
     2341 cattgagcat tgcagtttca acatcacata cacctgacaa tgaattcaat atcaactgca
     2401 atgggggaga tggtacaatt gatatcaccg ttaccggtgg aagcggtgag ggaacctatt
     2461 cataccagtg gacaaaatca ggagatgagt catggagcag cacacttgaa gacctgactg
     2521 tcaaagcggg gacatatcag ctgcaggtta ccgatgtaaa cggctgtgtc aatacaagga
     2581 gtgtggagat gaccgaacct gacgcattaa acctgtctct tgcggtatca gacataacct
     2641 gtttaaatgc tccgttatac aatgacggtt caattgacct gacagtcagc ggaggccggc
     2701 caccatactc ttattcatgg accggtccgg agggctttac atcatccctt gaggacatct
     2761 caggactgac agaaggtact tatacgatca ctgtcaccga tgcatacgga tgcagtattg
     2821 cctcagacac ccttctcagc ctgcctgaac caatctcgct tgaatcacgt gtttcagatt
     2881 ataacggatt cagcgtaagc tgttccggaa agagtgacgg atggctccgg ataattccac
     2941 agaccggcac ggctccgtac agttattcat ggagcggacc tgcagggttc accgcaacta
     3001 ctgattcgat ctactctttg agggagggaa cttattcagt aactgtaaca gacaagcacc
     3061 tttgctcagt gactcatgat attacactgg tttcgcccgg accgctgagt atgacgctgg
     3121 atattggcct gagcaacggc ggcagctata atatcaactg ttatggagct gcaaccggca
     3181 gggttaatat aacagctgtg aatgccgtcg gtacacctga atatatctgg tcagacggac
     3241 tgaccggagc tgccagggac aactttgcgg caggctatca cgaggtaatc atgacagacg
     3301 ccaacggctg cgttgctgac acctcactta cgcttacaga gcctgattcc atgcggatca
     3361 acttcagtct gatctcgccc ttctgtcctg aaagcaatga cggaagcatc tttgcaggtg
     3421 tcaccggagg tgagggtgca tacaccttta catggagcga cggacagacc acccaggaag
     3481 ctgttggcct ggtacagggg ctttacactg tagaagcacg tgattttaac ggatgtgtag
     3541 tttcagattc catgtcactt aaggcactaa acgacatctg tgtcggtata cctaatgcct
     3601 tctcgcctga cggcgatggg atcaatgagt tctggaacat cacaaggatt tcatactaca
     3661 gcgaagtcga agtgattatt ctcaaccggt ggggagagat tgtatggaaa tccgaaaagg
     3721 gatatcctga accgtgggat ggccgcgcct cagacggcaa ggtactcccg atggattcat
     3781 accattacgc gatagacctc cataatggag agaaaccaat tgtcggtcat gtgaccatta
     3841 tcaggtagat tatacgcaga agtgaaagag agcataaggt gaaaagggga ttgatcatat
     3901 tggctgtaat catgatggca tccgggcttg atgcccagca actgcccatt tacagccagt
     3961 atctgttcaa taagtatctc atcaatcctg ccgtagccgg cagtgatggt tacaccagca
     4021 ttaacctgac cacaaggcag caatggacag gctaccaggg agccccgcag acatattcac
     4081 tcagcttgaa cggcagactg ctgaagcaga agtacctcat acgtcagaat ctttttgaca
     4141 ggaaggtttt caggccacgc agtgagggcc gcgtaggtat cgggggaacg gtcttttctg
     4201 atattaacgg gctggtccgg agaaccggat tctccacttc atatgcatac catatctggt
     4261 tgcagggagg gacgcagctt tccttcgggc ttgcctttac gggctatcac tacaagatag
     4321 accagaggca gatacagttt gaagatcctg acgagccatg gctgaatact gatttcagga
     4381 aggggatatt tgttcccgac ttcaatttcg gtgcctatct tctgaacaga aaatacgcca
     4441 tcggattctc cgcgcaggag cttatggagg ggttcatcag ggcaggaagc caggcataca
     4501 gggacctgaa ggtacagagg acctattacc tctttggaaa ctatgacttt gagccttaca
     4561 tcaaaacggt gatccagcca tcactgctta taaagatgtc tgaacagatg aggcctcagg
     4621 cagatatcgg cattacctat tcatatgaca ataaactctg gattggggcc accttcagga
     4681 ccggcagcgc tatcatagcc aatgtgggtg tccggagaga caagctcttc tttgggtact
     4741 ccttcgattt taccctgaat tccattcaga tgtccaccta cgggtcacat gaattaattg
     4801 tggccgccag attcggagat accacgagga aataccgctg gcttgacagg tactagggtt
     4861 tcttttttga gacaacataa accagtgaac tggcctgctc tttccggaaa aggtttcgca
     4921 gagcgataaa gaggcctgtt gcaactcccc ttatcagtgg tatcgatttg ccttcgttcc
     4981 tgtagctcag acaggagata taaaaaagat caagcggcaa ggctgaaatc ttttcgcagg
     5041 caagttcgtg tctcccggca aatctgacaa aagctgcggg tgtgaaatgc cacagatgcc
     5101 tgggcacatc gagagctgcc cacctgtttc tgaaccaggt tgaatctgct gatgcgaagt
     5161 tgggaagagc gataatgcac ctgccgtcat ccctgaggat acggtttact tcacccatcc
     5221 acatatcagg atcatagaga tgttcaagca catgccagaa tgtaatgcaa tcagccgagc
     5281 cgggcggcat ctcccttatc tccgcaggag tcaccgccct gattgcaaat cttgctaccg
     5341 agtattccct ggcattatcg cttatctcaa ttcccgtaac ctgccatccc cgcttcttca
     5401 tgaatgcggc aaagtaacct gtaccgctcc ctatatcaac cagtgatccg gtctttttcc
     5461 cgacagccga cacaaccagc cttcgcttcc ggcttagcat aaagttgcgg gcgagatgat
     5521 agatgtagtc agcaatgctc tgtttcctgt cactatgcga gatatattcc tcagagagat
     5581 aatagttgcc tgcttcttct tcagggggag ggtcagcggt ccatcccata ccacagtcat
     5641 tgcatcgtct gaccagaaac tcactgttgc tgaccagatg atcctttgca tgaaacagac
     5701 cggcactgtc tgaagatccg catgcagggc agacaagttg ttcagccatc agccattcct
     5761 ctttttcagc aacggaaagg cagccagcag aagaaatgcc aggagcacaa cagatcctgc
     5821 gagggaaacc ctgttgccaa tcctgtacga ttccgggtca aacatgaatg tgacagtatg
     5881 gcttccggca ggaagggtca ttcccctgag gacataatta gccctgaaat gatcagcagg
     5941 tttgtcatca acagatgcct tccatccgta cttgtaatag atttcggaga atattgccac
     6001 cctttccgac gacaattccg ccttgtatgt cagcaggttg ggctcatatg aggtcaggaa
     6061 tattgtatcc ccgggtgttc cggcagtctc atcaactccg atcagctcag caaaacgcct
     6121 gtcaacaaca gcctcttcct tcggattgat ggttttgact gccagcagtt cagcatccgc
     6181 gttctctgca agtgttaccc tgttgaccag ccaggcgttg ccggcagcat gcctgtttac
     6241 cagtggagct gatccgggat cgagtataat atatctggta ttaagcatgt taagtatgcc
     6301 caggcttccc aacgctcctt ctgcttcctc catggtcgtt gccgttctga gattagttat
     6361 cagggagttg atgtcatctg acaggcttgt ctcaataagt tcctggtacc tcttcatctt
     6421 cgccccgctg taacctccta tggaatggtg gtggtatgac gtggaagcat cattgaaaac
     6481 cgaaacggtc aggtttagta ccctgaattc atcttcgtcg cccatgatga ttttgtctgc
     6541 ctcggatgga gcgaaggagt ttgcggatgc cctctttgtc tcaaagctgg atttgccgag
     6601 aaaccgggca tcaacaggca cctggtcaat gaggaagagc gctgtcatac ccagtagtgc
     6661 atatgcgggt ttgagtttgt tcttcaggta aaaccatata atggctgctg cgccggcaat
     6721 aaacagggat gacctgaggg catcggtacg aatcatcatc ttcctgtcgg ctgccattgc
     6781 atcctttatc catgcaacag aatctggcag tgccatctcg tcaggtcgca ggaatgatcc
     6841 tgccagaccc ggtatcagga agaatatgaa agccaggccg ccggtaatca tggctgcaat
     6901 atgcactgac ctggcagcct tctccctgtt tacacctcct tcggaaatct ccctgagggc
     6961 cagcacaccc aacagcggca ccagcacacc agccatgacc agggtcattg acaccgccct
     7021 gaacttgttg tatcccggaa agaagtcgat gaaaagggta gtcagtggca tgaggttctt
     7081 gccccatgag aggaagaggg agagcagtgc tgcagtcaat atccaccact tctcacggcc
     7141 tttgatcagc ataaggccga gcacgaacag gaatagcacc accgccccga agtaaacagg
     7201 cccgcttgtg ctgggttgtt tgccccagta cctgtagagc tgtccctgtg cctgacccat
     7261 gttgttcttt ctcagagcct ttaccgtctc cgagttgctg tcgaaaggtg ccgttgcacc
     7321 gcctctgaag ttgggtatca ggaaagtcat tgattcatct atcccataac tccattgggt
     7381 aatatactcc gggtcaaggc ctttctcact ggttcctgca gccgttgtga gttcactttt
     7441 tccacgggtt gactccttgc tgtactccca tgtggaatag agcgatgcaa aattgacgct
     7501 gaaggcaatc agtgctgcca ccaccagcac tcccagggat ttgaggaagg atggaatcat
     7561 cctgcttttc acagcgtgga ccagctcaaa gattccgaat acaagcacga tgatgccggt
     7621 atagtatgtt atctggacat ggtttgccat tatctggagc gacaggaaga gggcaaacag
     7681 tgaggctccg gtcaggagat tccttcggaa ggagtagacc accgagccaa taacaggtgc
     7741 catgtaggcc atggcatagg cttttgtatt gtgacccgca gagaggcaga caaagagata
     7801 ggaagagaat ccgtatgcaa ttgctccggc tatcgccagc catggattga ccccgaaaag
     7861 gagcagcatg atgtagaatc cgagcattga caggaagagc gctgccacag gtgtcttaaa
     7921 ggccttcagg aagttgtcca ggtgtttgaa aaggtttccc gggtatttca tcgagatcgt
     7981 gtaagcaggc attccgccga acatagagtt ggtccatagc ggttcaccgc cgtgtttctc
     8041 acggtaatcg cttatctcct ttgaagatcc ctcgtacact ttggtatcat gggcatttat
     8101 cttcttcccc tccagtaccg ggaagaaata ggcgaaggag atcacagtga acaggagcac
     8161 ggcaataata tgcggcaatg ctgatttgaa gctattcggt ttcatttgca gggtgttggc
     8221 aggtcatata cccggatttg aattatctga caaaaataca ccaactgatc tttaattcaa
     8281 atgagtatat tgttttacat ttgccgcaac ggcgaagcaa catgggcata gttcaaaggc
     8341 agacaatacg cgggaccgcc tggtcttacc tgggcgccct gcttggcttc gtgaatataa
     8401 tcctcctttc acccaggata tttaccacgg gagagattgg cgtggttcag ttgcttctct
     8461 cttttgccac gatgctggca cagttctcaa gcctggggtt cacaaatgtc atcaacaggc
     8521 tgttcccata tttccggggt acccgtggca ggcatcatgg atttctcggg cttgctgttg
     8581 ttataactct tgcagggttt attattgcgc tgattttcct gaagttttac gcacctcatt
     8641 tcgagagggt aaacctcgag cgctcgccgc ttatctcgag gtattccata tatctccctg
     8701 cattgttgct ggtaacgttg ctcttcaatc tcctggacaa ctacaacaag gttctttatg
     8761 atgctgtgct tggaacattc ctcagggagt ttttcttcag ggtgcttaac ctggggctga
     8821 tcgtgctgtt ctgggcagaa ataattgatt ttgacggtta cgtcttctgg tatgtggtct
     8881 cccagggagt gctgctgatt atcattttca tcagcctgct tatgaggggt gagatctcac
     8941 tcaggctcga gcccggattc atcaccccgc atctgagacg cgagatcata ctgttgtcgt
     9001 tgttcggcat actgacaggc ataagcacgg tgacactgac aacccttgac aagctcttta
     9061 tcaataccta tctcggcgag gccgaggtgg gaatttactc tattgcctca tattttgcag
     9121 tgatgatcac actgcctggc cggtcagtat ccaagatatc gattcctttt cttgctgagt
     9181 catggaagaa gaacgacctt gaaaaaattg acgacatgta cgtgcgcagc agtgtgagcc
     9241 agtatgctgt cggtcttctg atctttatcg gccttctggt caacattgac aacatattcc
     9301 gcctgctgcc ggatatatat tcaaagagtg cggcagtaat agtactcata agcttcggca
     9361 accttgtaag cggttctgcg ggtgttaacg gtgtagtgct cagtacttca ccgctttaca
     9421 ggtatcagac ctggcttatg ttcattctga tcggtctttt tgtcggaacg agcctgatct
     9481 tcatccctct gttcgggata accggtgctg cgcttgcatc gatgatttcg aatattatct
     9541 acagcatgct gggtgttttg cttaccggca caaggtttgg cctctggccg tacagcatgg
     9601 tgcacctcaa gatgacgctt gtcgctcttg cagcacttgc ggcagggtac ttcctgccgc
     9661 agatgccgct tattactgac atcattgtaa gaagcttgct ggtgacaatg atttacaccg
     9721 ccggggtttt gttctggagg ctctcggatg acctgaacgg catagctgaa tcttttgtgg
     9781 agcggttcag gcggagacgg tagaatgcgt gcctgcttac gtgtaacctg tgagaggtgc
     9841 ctgccggcag gaaccagacc agccggttaa tgctacagcc tgtcgagaat atttcccagc
     9901 ttcactgcca gcgccctgcg cgaatactcg aggtgataac cgctccgctt aaccggcttt
     9961 ccctctgcaa ctctgaggat gaactccctg atgccttcaa catcatcata cccgaagata
    10021 ccctggtagc cgcagagtgt cagcaacctt gcagcatcgc catcttcggg tccgaggaac
    10081 agaatgggct tctctgttgc aaggtattcg aaaatttttc cggtcaggat tgacctgttt
    10141 gaactgtggt caggaattat aagcagcagc aggctggaac tcatcatata ccttattgct
    10201 tcaggatgct cgcaataact tataaaccgg gggccatcgc cctcgtcacc tgccgggaaa
    10261 agagccctga tctcttcggg aacagtaccg acaaactgaa gtgccacctg cttcccgtta
    10321 atgcgcatat ccttcacggc gtcaatgaac ccccggaccg gataggcagg tgagagggta
    10381 ccgacatagg taatggtgaa tctctccggc accgatgaca ctattccctg gaagtcctcc
    10441 tcgtcaaatc cgttgggcag tatctctgtt ttaccagcca ccgcctctgt ctttgcagct
    10501 aagagtgatg ccagtgtttt tccgacggta atgatcctgt cagccctgga aagcactctc
    10561 ttctcaagat tcctgtcgag catccttgcc ggcagcgaag gcctgaacat gtcatagtaa
    10621 tagatgtcag tccatggatc cctcagatct gcaacccatt tcagcccggg gaaccgacgc
    10681 ttcagcctca gtccgatgag ctgggtggag tgaggagggc tggttgtaat aatatgagtt
    10741 atgttttgtg atctgattag ttcggatgcc ctgcgaaagg cgaacctgtt ccatcccctg
    10801 cgggggtcag ggataaaaaa atttcccctt atgaatctgc tgacagtgct tttaaatccc
    10861 ttatcctttc ccgaggcaaa tccggcatga ggtattttag actgatccct gttgtagagt
    10921 gcaaaaaaat tgagggcttt cgtacggtgt actttcacat ccaccgggac ttcgccgtac
    10981 agggagtcat ccctgaaggg ataggctgca tatagcgggt caacggtaag cacttccggg
    11041 aaccagcctg atgaagggag gtatttggtg aacttgagcc agcgctggac tccggccccg
    11101 ccactgggag gccagtagta ggtaatgatc agaacctttg ccggcacggc tctcatatca
    11161 aagttttttc agttgttcct gcatgctgac cttgtcagcg atgattgccc tgagttgcga
    11221 aacagaaaca cgctcctgct ccattgagtc acggtacctg atggttaccg tttcatcctg
    11281 cattgtctgg tagtcaatag tgatacagta cggagtacct attgcatcct gtctgcggta
    11341 gcgcttgcct atcgagtcct tttcatcata ctggcagttg aaatcgaact tcaggtcgct
    11401 gataatcctg cgtgaaatat ccgggaggcc atccttgcgc accaaaggca taaccgccag
    11461 cttcaccggg gccagcgctg caggaaggcg gagtaccacc ctgctgtcag gcgtaccatc
    11521 ctcctttgtg agatcctctt cgaagtacga ggcagcaatc acctggagga acatgcggtc
    11581 aacaccgata gaagtttcaa tcacataggg tatatatgat tcgtttatct cgggatcaaa
    11641 atactgcatc ttcttgccgc tctgttgctg gtgctgacga aggtcgaagt cagtacgcga
    11701 gtggatcccc tcaacctccc tgaacccgaa tgggaacctg tattcaatgt cggctgcagc
    11761 gttggcgtag tgggccagct tgtcatggtc atggaaccgg tagttgtcgt ctccgaaccc
    11821 gagggctctg tgccatttca tccttgcctc cttccagtgg ctgaaccagt caagctctgt
    11881 gccgggcctg acgaagaact gcatctccat ctgttcgaac tcgcgcatgc ggaagatgaa
    11941 ctgacgcgcc actatctcat tccggaatgc cttgcctgtc tgggctatcc cgaaggggat
    12001 cctcatgcgg ccggtcttct ggacgttcag gaaatttacg aagataccct gtgccgtctc
    12061 cgggcgaagg taaattttca tcgaaccgtc agatgttggc cctgtctccg tggcaaacat
    12121 caggttaaac tgcctcactt cagtccagtt gcaggtcccg gatactggac atacaatgcc
    12181 gttgtcaact attatctgcc tgagctctgc cagatcaggg cctgccatgg cttcaacgaa
    12241 tcttttatgc agggcatcat acttttcccg ggtctcgagt atgcggggat tagttgagcg
    12301 gtaaagggcc tcgtcaaatt tttcaccgaa cctgcccctg gctttcacaa cctccttttc
    12361 aattttctca ctgagttttt caagatgctc ttctatgagc atgtcagcgc ggtaacgctt
    12421 cttgctgtca cggttgtcaa tcagagggtc attgaatgcg tcaacatggc ctgatgcttt
    12481 ccagatggcc gggtgcatga atattgcgga gtcaagaccc acaatatttt cattgaggag
    12541 aaccatgctg tcccaccagt atttcttgat gttgtttttc agttcaacgc catactggcc
    12601 atagtcatag acagcgccca ggccatcata gatttcactt gagggaaaca catatccata
    12661 ctctttgcag tgtgcaacga gttttctgaa aacatcttcc tgagccatat tgattctgag
    12721 ggtcttcgtg ataacaaccg ggcaaaagta tattaaaatc cgcggtttta caatccggtt
    12781 tcatgtctga aatcaatttg tatctttgct ttttttctta gcaggccttt atgaaaatca
    12841 ggtgtgatta tcttgtgatt ggttcgggta tagccgggct ctcttttgcg ctcaaggctg
    12901 cagcccacgg agaagtatgt gtgatcacaa aggcgaagat ggatgactgc agcactaact
    12961 atgcccaggg tgggatagca gctgtgatgt atgagcctga caactttgag aaacatgtaa
    13021 gggacacaat gattgccggt gccggatact ccaatgagga ggtggtgaga atggttgtca
    13081 gcgaagctcc ggaacgcatc agggagctga tagagatggg cgtggatttt gacaagaagc
    13141 ctgacggtac ccttgacatg gcaatggagg gaggtcatac cgagcacagg atacttcatc
    13201 ataaggatta tacaggcgct gccattgaga aggtgctgat taaaaaggca cgtgagcaca
    13261 aaaacatccg gatctatgaa gatcattttg ccattgacct gctcacccag cataaccttg
    13321 gcgagtatgt gacccgggag tggaagaata tcgagtgcta cggagcctat gtggcagacc
    13381 tgacaagcag aagagtaatc acatttcttg ccagggttac ggtgctggcc accggtggtt
    13441 gcggaaacat atacctgacc accaccaatc caccggttgc cacaggcgac ggggtggcga
    13501 tggtacacag ggccaacgga gtgcttgaga acatggaatt cattcagttt catcccacat
    13561 cactgtttaa tccgagggag agaccgtcat tccttatcac tgaggcgctg aggggtttcg
    13621 gggctgaact gcgcaacagg gccggagaga agttcatgcc gaaataccat gcacaggggt
    13681 ctctcgcacc gcgcgatgtt gtggccaggg ccatcgacaa tgagatgaaa ctgaagggtg
    13741 atgactttga gtggctcgat tgcacccatc ttgatgctga cgcgctcagg tctcatttcc
    13801 cgcatgttta tgagaaatgc ctctccgagg gtattgacat aaccagggac atgatcccgg
    13861 ttgtacctgc agcccactat tcatgcggtg gtgtaagggt tgacaggaac ggaaggagct
    13921 ccattgacag gttatatgca ataggagagg tctcatccac cgggcttcac ggtgcaaaca
    13981 ggctggcatc caactccctt atcgaggcag ccgtatatgc ccacagggcg gccagggatg
    14041 catcggcacg cataggctca ctcactcatg aagaaaagat tcctgtctgg aactatcatg
    14101 gcaccaccca cctcgaggag atgatcctcg taacacaaaa ctaccgggag atgcagatga
    14161 taatgagcaa ctacgttgga atcgtgaggt ctgatctccg acttgccagg gccaaagcac
    14221 gcctcgagat tatctaccag gaaacggagg acctgtaccg gaggtcaacc atttcacggg
    14281 agctgataga actccgaaac ctcatcgctg cgggatacct ggtaataaag atggcccaga
    14341 acaggcagga gagcctggga ttacactact cgatagatta cccggcgaga cccggatctg
    14401 aattttaatg acaagttata tggaagtcaa tgaaacaatc gacacccctg aggccggggg
    14461 cgagtcacct tttatcagcg ctgcttttac cgggaagaac tcattctgga ggtatttcac
    14521 aggagtgata gctcctttcc ttgctgcaat catcataagc ggactgctgc tgaaggcagt
    14581 catcatagcc agttctcctg acggctatgc aatgctacag aaagatacca tgcctgattt
    14641 tgaagccatg ggcattaatc tcaatttggg attcgttctc aacgtgtttc ctttcctgat
    14701 tgggttgctg gcattcatcc ttctggtcag gccattgcac aacagaacat tcgggagtgt
    14761 tatcaacggc ggccggaagg tcaggtgggg ccggatgatg tttgccgccc ttgtatggat
    14821 cgccgtatca gcactctact tgctgtatgc actgaaaagt gacccgggaa acttccggat
    14881 caacaacaca tcaaactcac tgattgttct tgctcttctg gcattgttgc tgataccttt
    14941 ccaggctgct tttgaggaga ttcttttcag gggttacctg atgcagggtt ttgccgtcct
    15001 ggcacgcaac aggtggatcc cgatactttc cacatcaata ctgttcgggc tgatgcacgg
    15061 atttaacccg gaggtcagta aatacggttt cctcacgatg atgccccagt acatcttttt
    15121 cgggcttctc ttcgcagtac tgacgatgtt cgatgacgga attgagctgg caataggtgc
    15181 acatgctgca aacaatattt tccttacggt gcttgtcacc catgaagaca tggcccttca
    15241 gactccggca ctatatgaac agatacagat ctatccatgg gaggaatttg gcgggctgat
    15301 tctgcagtcg ctgattttta ttgcaatact ggcacttgtg ttcaaatgga aggatatacg
    15361 caagctctat gcacggatca ttgttccggc tgcggaagtg tcagatgccg tagatgccgg
    15421 ttgaccgttc ataagtgaaa tcaccccatt tttctttgat ttcaccgata attgagcgta
    15481 tctcatccac gtcggttgat gtcaggagct tcagcctggt ctccttaaac ccgtgaaggc
    15541 ccttgaagta atttgagaaa tggcgtctca tctccaggat ggcaggtttt ccttccttgt
    15601 gttcaagtga tttatcaagg tgcagcagag ccagatcagc cttttctctc acggtgggct
    15661 cgggaagtat ctcaccggta tcaaggtaat gtcgtatctc cctgaatatc catggtcttc
    15721 cgaatgttgc ccgtcctatc atgataccgt caaccccgta tctttcaaac atctctttcg
    15781 cccgttcggg cccgttgata tcaccattgc caatgacagg aattttcatc ctcgggtttt
    15841 tctttacctc gcctataagg gtccagtctg cctcgccccg gtacaactgt gaccgggtgc
    15901 gcccatgaat ggtcagtcct gcgatacctg tatcctgcaa acgctcggca acatcaacta
    15961 tgattttgct gttttcatcc cagccgagcc ttgtctttac cgttaccggg gttttcactg
    16021 ccctgatgat ggctgaggtc atctctgtca tcagcgggat attctgaagc atacctgctc
    16081 cggcgccccg ggcggctatc ttccttaccg ggcatccgaa gttgaggtca atgagatcag
    16141 gatttgcctc ttcggcccga agggcggcat caaccattga ttcggtgata tggccgtaaa
    16201 gctggatacc tattggccgc tcataatcga agagatcaag ctttttgacg cttttgcggc
    16261 catcccttat cagtccgtca gatgagataa actcggtata catgaaatcg gcgccgaaca
    16321 tcttgcagac atacctgaat gaggggtctg tgatatcctc catgggagcg agaaagagtg
    16381 gttgctccct gagtatggta tttccgagct tcaatgagtg attactggtt ttcctggttg
    16441 gcgctgaaga gggtgagaga gagatttttc ctggtcttct catgttttcg gaatacatag
    16501 acaagaccat atttctcagc gttggctctt ttctcctctt caggaagtga cgagaagctg
    16561 gcttcgatct cgttccagag cagtgttatc agttcatcag cctcctgacg catgcctgcg
    16621 gtcttgttca cgcaatcaga tgccctccgg ttgacagtct tgcaatggct ccgcgtctca
    16681 agaaactttt cgtaccgcac cttgacaact gctatggtag gattggttac aggtgaataa
    16741 ccttttctga tccggtattc ctccccgtca atgatccgct tgccccatgc caccagttca
    16801 ttttctgttg ccagggaagg aagggtgctg tcatccgaag caatgccata gaaagtcctt
    16861 gtttcaggcg gaagctcacc ccttgtcact gccatattca tgaccctcag gaaatgagta
    16921 aggtaaagcc ttgccttgcg tgccgtctcc tggtttgact ttgccttctc attttggttg
    16981 acaagtgtct gcctctggtg gctgatgctg ttttcaaaca atggcagaaa tgcctggatc
    17041 ctgacgaggg tcttcataga gaaagcgagt ctgaaaggcg gaatctcctt gctcatttca
    17101 atagctttct taaaggctct cagacgcgca gaatctgtat tgggcaggcg gcgatagggc
    17161 ataaacgggt gatttttgtg aagttaatat gcgaatatac gttcttgtgc gaatctaaca
    17221 acactcttcg caatgaaaaa tgactttaat aagctaaaaa atccgttgct cttgttacat
    17281 tagcgtactg gtacataaaa caaattaaga tatgtttgaa aaagaagttt acattaagcg
    17341 ccgccaaagg ctgaagtcac tggtgaatga tggtatcgcc cttttcattg gcaatgttga
    17401 agcaccgatg aactacccgg acaataccta tcactggaga caggatagtg attttctcta
    17461 ttttttcggc attgatctgc cgggactggc aggagtgatt gatttcaatt caggggatga
    17521 gattattttc ggcaacgatt ttaccgttga cgacatagtg tggatgggcc ctcagccctc
    17581 aattgccgag ctggcatccc gtgccggtgt tgtgcttacg catccgatga ataagctgga
    17641 tgaaatggtc catgatgccc tgaaggcagg aaggccaatc cattttctcc cgccataccg
    17701 tgctgagaca aagatgaccc tgggtgcact cctgaaggaa aacccctgcc agatgcgcac
    17761 gaaggcttcg gagaagctca tcaaagccgt ggtttcattg aggtcaatca aggaagatat
    17821 tgagatagtt gagattgaaa aggcaattga cataacatgc gagatggaga caacggctat
    17881 gcgcatgtgt aagcccggag taacagagca ggagatattt ggtgtgttgc agggcatagc
    17941 ctactcccgg ggagccggcc cctcatttcc gataatcctg agcatcaacg ggcaaaccct
    18001 tcacaaccat gtgcatatta atgtgctcag ggaagggagg atgatggtta ccgactgcgg
    18061 agccgaatca aatatgcact atgcggctga cctgacccgt acgactccgg tgggagggaa
    18121 attcagccag cgccagaagg agatatatga aattgtcctc agggctaaca ccgaatcaat
    18181 aaaaaatgcc ggaccgggaa tctcaaaccg cgaccttcat cttggtgcat ggaggataat
    18241 tgcagaaggc atgaaggata tgggcttcat gaaaggtgac attgatgagg ctgttgcagc
    18301 aggtgcaccg gctctcttca tgccgcacgg ccttggccat atgatgggtc ttgacgtaca
    18361 tgatatggaa agcctgggag aggattatgt gggctataat gacgaggtga agcgcagtaa
    18421 ccagttcgga ctgagatcac tccgcttcgc gctgccatac aagccgggtc atgtctttac
    18481 cgttgagccg ggaatctatt tcattcccga gcttgttgcc aaatggaaag ctgaaggtat
    18541 caacgaagcc ttcatcaatt tcgcaaaggt tgagagttac cttgattttg gcggcataag
    18601 gattgaagat gatgttctca ttacccccac gggttcgagg ttactgggta aaccggcacc
    18661 gaaaaccgtt gaggagattg aacgcacctg catttaaaat attgccccac aggaaaatac
    18721 gcaattggtt gatgtagcgc cgggtctgag acccgtcggt acgtggccgg atggaaaata
    18781 tgtaaatgat tgatgtagcg acgggtctca gacccgtcgg tacgtggccg catggaaaat
    18841 acgtaatggt tgatacgtgg ccgcatgaaa aggaatatac aatttgaagg tgtaaggata
    18901 tattacacgg tgcgcggcaa ggggaggccc attgttttgc tgcatggata tcttgaaggc
    18961 ggagaggtat gggatccact ggctgagaag ctggaagaag attaccggat catcagccct
    19021 gacctgccgg gtcatggtga atcaggggtt aagggtgaag tacacaccat ggagtttctt
    19081 gcttcagcag tgcgcgaagt gatcagggat gcaggggaga agagggtagt gatggttggt
    19141 cactccctgg gtggatatgt cacacttgca tatgtggagc tttacccgga gatgctgtca
    19201 ggatatgtgc tctttcactc tcatccccac gctgacacac ctgaggcaat tgcacgccgg
    19261 aagagggaga ttgccatcgt cagggccgga aagaagaaca ttatgtatcc gggtaatgtc
    19321 agcatgatgt tcgcgaagga gaatctgaag tcaatgcatg cacagctgga aaggtcccgg
    19381 cagattgctt cgcggaatag cgttgagggg attattgcca ttctgaatgg aatgatagtc
    19441 aggccttcac gccagtatat ccttgaaaag ggggaggttc cgctcttatg gattctgggc
    19501 aggcatgatc tttacttctc tcccgggaag gcccagtatg atatcggcct gcctctcaac
    19561 gcacgggtcg tcatcctgga gaactcaggc cacctcggtt tcatcgagga gacagaaaaa
    19621 tcagctttac tgctgaagga ttttgccggc cggttaacct ggtgatgagg tttcagttgt
    19681 tttccaggta actgaaggct tccaggaggg ttttgtcaat ctcctgccag ataggataga
    19741 atcccttgtt atcaagcata ttacgtgcga tatatgcttt cagctggact tcaattatgt
    19801 tctttgaggt ctcaatctga tcctttctgg ccggtacgcc attttgtccc gcgtagccaa
    19861 caaattttga aaggagattc tgaccttcga gccatcttat cagctccggc agttcattgt
    19921 attttttgag ggtctcacgg ttgtcctcgg tgaatttcag ggcatacctg tatagcaggc
    19981 cggagttcct gacagcaagg aaatactctg aaataccggc ggtgtcaatt gggataaaga
    20041 tatcaggcat gattcctcct ccgccgtaga ctaccttgcc tcccggggtg gtgaagcgca
    20101 gtgagtcatc aagtcttact gaatctgcct cctcaaactc accgtgcata agccggtagc
    20161 caagatcttc ataatacttg tcaactccct ggtcataggg tttctgaata gaacgaccgg
    20221 taggggtgta gtagcgggca attgtcagtc tcatgccgga cccgtcacgg aacatgacag
    20281 gctcctgaac gaggcctttg ccgaatgagc gtctgccaat gatcgtgccc ctgtcgttgt
    20341 cctgtaccgc tcctgccagt atctcgctcg atgaggcgct ccactcatct atcagcacca
    20401 caagctctcc cgtctcaaat tcgcctttgg cggtggcctt catctcctgg cggggtgtag
    20461 atttacccat ggtgaagacg atgagctttc cttccggaag gaactcgtca gcaatctgga
    20521 ttgcagcatc catcacacct ccggagtttc ccctgaggtc aaggatcagt tttttcattc
    20581 ctgtctcctt gagctcattg aggccctttt tgaattcatc atgggtggtg agtgcgaagc
    20641 tggaaatctt gatgtatccc acatcatctg acatcatgta tgatacatca acgctataga
    20701 gtggaatctt gtcgcggata atggtgaaag gcaggagctc cttcgcgccc ttgcgctcaa
    20761 tcttcaggtc gaccctggtg ccacgcggac ccttcaacat cttgaccaca tcctcatcgg
    20821 gaatgtttct gccggcgatg agtgagtcat tgaccatgat tatcttgtcg cccggtataa
    20881 tgccggcctt ttctgagggc cctccggata tagtgctgat tataagcaca gtgtcagtca
    20941 gcatgttgaa tgaaacacct attccgtcaa agttgccctg cagcggttcg ttcgcctgcg
    21001 cgagatcagt ggcagggatg tagactgagt gaggatcaag tttacggagc attaccggta
    21061 tggcctcctc tatcagcgcg tttctgttga cggtatcaac ataatcagac tcaatgatgt
    21121 caagtatccg gttcagcttg tcatttgacg gcgggaagtt tgtatagaac ccggcctggg
    21181 gatggggcat ataaaaacct atcaatatgc caatggcaat tgagattccg attatcagcg
    21241 gaaggtacgc cttgttatat ttcatttttt ctttctccaa gatcaatata aagaagttcg
    21301 atgccagccc gtttaagtaa ctcaatacca tcaatattgc ggtactggtt gctgtagact
    21361 acccgcctga tacccgactg tatgatcagt tttgcacact ccatgcaggg tgagtcggtg
    21421 atatacagtg tggcgttctc acttgagttg tttgaccgtg ctaccttggt gatggcgttg
    21481 gcttcagcat gtaaaacgta tggcttggta acattattct catcctcgca gatattttca
    21541 aaaccggcag gtgtgccgtt atatccgtcg gagataatca tcttgtcctt gactataagt
    21601 gctcccacct tccgtctctc gcaataggag ttctcagacc atattgaggc catgcgcagg
    21661 taacgcctgt caaactgctc ctgtttttcc ccgtagggat ggatggcatc cttttttatc
    21721 atattcttct gtttcgccgg caaacttaat ggtttttaat ctaaatcaga taaagagctc
    21781 gcctttaaga tgcagcggtg ctgagcctgc gattcgttcc tgtctccgtt gagggtggca
    21841 tggccgcaca ggtcaacctc ctgcatcggc gtgaaccacc ttatcagcca ttcatccttt
    21901 ccgcgtgtga taaatgcagt ttcaggcaga tggttttcca tagctgtctt ctgcattgtt
    21961 tcttctgtca ccgggtcagt aagaacgcat actgcagcag ggttgccagt gattggaacg
    22021 tgagtgaatg catcaacctg gtaaatctgg atggacttca ttcaggctca tttttgcagc
    22081 cctaaagtta ccattgttta tcacaaattg ataaaggatg aaacttgagt attatcttag
    22141 tatcatgaat aggatccata acacgcagaa tggcaccacg atcacgaggc tgacgcctgc
    22201 cgggtgcaat atatgggttg ttacgcggaa cgaccggacg attattgttg atacaggcat
    22261 ctcgtttgac aggagtagga ttgaacgggc gctgaagcgt aagggaataa ttcctgaggc
    22321 ggtgatcctt acccacactc attttgacca tgccggcaat gcagcctggc tgcaggcgga
    22381 gtatggtagc ggggtggtgg tgcaggaggc cgagagcggc atgcttgccg ccggtgatac
    22441 accgataccg gaagggacct acaccttcac ccgcggcatg attggaattg gcaggaagat
    22501 gcagccggtt ttcaggtatg atccctgtgt tgccgaacat gttttcaatg atcagtttga
    22561 tctgaaccgt tttgggataa acgggtatgt aatgcatacg ccgggacatt cgccgggagg
    22621 agccacggtg gtgattgatg atgagacagt cattgtaggc gacctggtag ttgggaccat
    22681 gcccggctca ccgttcccgc catttgccga tgatgtcacg ttgcttctca acagctggaa
    22741 gaagatcatc gacaccggct atagatccat tcttcccggt cacggcaggc cgatcagcag
    22801 ggaggagttg ataaaggaat acgataaaag aaaatagaga tggcaggacg atttatttgt
    22861 atcatacttt ccgggatgga gtggctgtct cgcaaagggt gggagttcag gttttatctg
    22921 tgcatcccaa cttactcgct gatctctcca gagtgacttt catggtggga ggttgggttt
    22981 tatctgtgtt tccctctcac agcctgagtt tgatatgcat cctggcaagg atcgccgtca
    23041 gccccgacat tagcagcgcc caggcaacgg agccgaggat aacaaccagt ggttctgacg
    23101 actgcttgta aatcagtacc ggaacggatg acatccatat tgcgtggtag aggatattgg
    23161 gggcgaggta tgtggtcagt gaatatctcc cggcaggtct gacgaaatca gtccatccgg
    23221 tcagccctct cacatcggca agccagtata ttgcagcaaa gaccaggaag ctgatgccgc
    23281 tgcagatcat cccccagctg ggagtggcca ggagcttgga gatgataaac cattttcgca
    23341 agaagaatcc cagggcgagg gagattatgc ccatcactat tagcatcaaa atgatttttt
    23401 catctccgga ttttttcagc ttcctgatca gtataccggc aaacatgcct gtaagaacta
    23461 tcagcgggac attgccctgt atgagtattc caaggaccgg cctcacggga tcgaggaatc
    23521 cgagcacacc tgactggtca aggatattga tcatcaggaa gaagatgacg gcagccatgg
    23581 tccatgccac tgaatcacgg aatgccacat aaatcagcga tgcggtcagg taaccccatc
    23641 ctatgaggcc aaggattccc caccagcctg ttatcatcca gcccgggtca tcggcgctgc
    23701 ctgaccggaa aataattgcc aggaagatca gtgtagcgac gccggcagcc ctcagtattg
    23761 tgaaaagcag tttgtgtttc cctttagggt agtcattcca taccaggaag acagagatgt
    23821 acatgagcaa tgcccagagg tttctgttca tgccggtggc ctcgggattg actcttccgg
    23881 agttcagcat caggatcccg attaccataa ggcttacagt gcggattgct atatgaagga
    23941 gtattccgga aaccggctca ttccttttta ttctgcctga gatggcgaac ggtatggcca
    24001 tgccaacgat gaagagaaat cccggaaaaa cccagtctgc gaggcccatg ccatcaaagt
    24061 cagcctccat atgcccgagc caggcaggtg ccaccttcat gttcaggtca ttgacaaaga
    24121 gcatgagcag gagagtcagc ccacgcatga tgtcaacaga ggcaattctg ggtctgtttt
    24181 ccataat
//
LOCUS       DAOPBK010000011        22693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2422_length_22693_cov_4.954457, whole genome shotgun sequence.
ACCESSION   DAOPBK010000011 DAOPBK010000000
VERSION     DAOPBK010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22693
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2422_length_22693_cov_4.954457"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLA17_01405"
     CDS             complement(<1..295)
                     /locus_tag="PLA17_01405"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPK84099.1"
                     /translation="MEAFRSLFAGPGIATTILYLSITAFTGILLGRINVKKLRLGVAG
                     VLFSGLLLAHFGVRADLHVLTFVRDFGLILFVYAVGIDVGPRFFSSLRREGLKL"
     gene            complement(453..1406)
                     /locus_tag="PLA17_01410"
     CDS             complement(453..1406)
                     /locus_tag="PLA17_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPK84100.1"
                     /translation="MTSVIILRQIGILTAIVVIGLIACRAGVITAGTKDFLAKLIFYV
                     TLPSMLFTSFSAIEVTPRLLANSLQALLLAVLVILFMLFAGWLTTVIFGIKGSHGSIF
                     RLHSMLGNIIYLGLPVISAQFGQEGVLYGSMFVLVSNILMWTLGVGILTPAGSFSLKS
                     GMKKIFNINTIAIIAGFLLFLLSVKLPQFILDSVGALGKTNTWLSMIFIASVIYFADG
                     RKMLRNRNAYLLSFNRLLLVPFILLGLFILFDLLFPGIIRKEVVSVMLMQAAMPCMVN
                     VVIMVSILGEDDGTATANVFVSTIFSVITLPIILLSLSLLG"
     gene            1597..2274
                     /locus_tag="PLA17_01415"
     CDS             1597..2274
                     /locus_tag="PLA17_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPK84101.1"
                     /translation="MKILIIEDESRVASFIKEGLEEKSYKTVVAYDGITGEEAAMKDD
                     ISLVILDLIIPGKSGIDVCRSIKAVRPDLPILMLTALSTTVDKVRGFEAGADDYLVKP
                     FDFPELLARIKSLLKRSRNSTHQTQSLRVADLELDLMRKVAIRGGKVIELTAKEFALL
                     EYFMRNSGRVLSRADISEKVWNITYDTGTNIVEVYVNILRKKIDRDFEPKLIHTRIGL
                     GYIFTDE"
     gene            2275..3657
                     /locus_tag="PLA17_01420"
     CDS             2275..3657
                     /locus_tag="PLA17_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK84102.1"
                     /translation="MKIRLKITLQFFLMASVILLLFSLTVYIVSATYRRDDFYQRLES
                     RAISTARLLIEVDEIGTDLLNKIEEDNPVSLPDEKIIIFNYKNEIVYSTDTRNVILYD
                     SDLLTRIRASGQIKTKSGRYELMGILFTEEFNRFVVLSAARDIYGFDKMKNLRTTLIL
                     LTGIFLLLFYPAGWLFAARSLSPISKVVDRVEEISIASLDLRVDEGNGKDELAHLAHT
                     FNRMLERLETAFEVQKDFISNASHELRTPLTAIRGQLEVLLMKERTDKEYRDSTAAVL
                     EDISRLNQLLDRLLLMAHASSEGAREGFREVRIDEILWQAREELLKHEKEYTVTVALD
                     ENISDIENLTMRGDEQLLISAVTNVMENGCKYSPDKSVRVLLSHDADNIFVSFTDNGP
                     GIAEDEIERVFESFYRSKATASVRGHGIGLSLASKIIDIHNGTITVKSGQGEGTTIRL
                     AFPAERSVAV"
     gene            complement(3878..4504)
                     /locus_tag="PLA17_01425"
     CDS             complement(3878..4504)
                     /locus_tag="PLA17_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84103.1"
                     /translation="MNKGTIGLNKEKTRDQAVVRKSPRKLSLRDSGLMHLFDELDLEV
                     RQGLIDYLDRKGLVKGSGMLVIPSSRHYFYDAEDMKGIRTVINLRQLNYIREIRDFLR
                     QITGLLPAKSNFIGCFIDNRSHGAGSDKNAGSSGHASDKADAYENGIESRIPFINRMY
                     SIIDLRTNRYLTRRTVANLLKESGLELVGMTEINGFTYFHARKSNPAA"
     gene            complement(4770..5048)
                     /locus_tag="PLA17_01430"
     CDS             complement(4770..5048)
                     /locus_tag="PLA17_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPK84104.1"
                     /translation="MGLKVHYRIHVTGMVQGVGFRWSVAIEAWKRGITGYVKNIPDGS
                     VVIEAEGAREPLEEFAAWCRHGPGTWLVDNARVTEGPLAGYREFRIER"
     gene            5167..7278
                     /locus_tag="PLA17_01435"
     CDS             5167..7278
                     /locus_tag="PLA17_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPK84105.1"
                     /translation="MKKGWKIVIVVLGIIIFIPLFFISSVQIGVFGRLPGKKELISFR
                     NATASVVLSHEGETIGRFFSENRITIPFDQMPAHLINSLVATEDARFYEHSGIDSRSL
                     VRVVFKSILFSDRSSGGGSTITQQLAKNMYGRKDYRVFSVILNKTREVILAKRIEKVF
                     TKDKILELYLNTVSFGENIYGIEAASQRYFSKKAADLKVEEAALLTGMLKANTMYNPR
                     LYPQNAVRRRNVVLGQMEKYGYLEGRAADSLSALPIKLDYNKTDLSGPADYFMVRVRI
                     ETDRILNEISSFRGKEWDIERDGLIITTTLRMPLQQAAGEAFAAHMPRMQKRLNEQYS
                     TSSGRRALKGIPDSLKKMMTTLHAGLLAMDPATGAIMAWNGGIDFKTQPYDQILARRQ
                     LASVFKPFIFAAALEDGVEPCRYLDNDSVTLSGFNDWSPENYNHSYGGKYSLAGALAQ
                     SMNIPTFSLFLMIGFDKVDRLWQEMGFSFPLVNMPSLAMGTAEASILEVARAYAAFAN
                     GGMLVTPYLIESVKAPDGRILWQYMPPRRSDRVLSERSATLMAAMLEKAIREGTGASV
                     HSAYGVKVPLAGKTGTSQNYADAWFAAFNPAMVIVSRVGASTPSIHFNSGSYGSGSAL
                     ALPLVALTLKKVEKNKEIMSVINTPFAPLPPELEMALDCPDFREKSFFDRLFDIFRDN
                     NIDYQKETRRRRIFRGRIFRI"
     gene            7436..8263
                     /locus_tag="PLA17_01440"
     CDS             7436..8263
                     /locus_tag="PLA17_01440"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HPK84106.1"
                     /translation="MMKKNAILLLAAALVLISGACNEIRNGDGTGRVILKVTDAPFDI
                     NSIESALVTITKVELREECDGISDACEFITLSEDTVTLDLIDLRNGVTETLLDMEIPA
                     GRYDMVRLYIYEAGLKLKDSELIHRLKVPGGSQSGIKVFLRPVLEVAEGSLEEILLDI
                     DLSRSFILRGNPAHNNGFIFKPVVRAANMAKAGCIRGVVTDTEGTEISGASVWIEQDT
                     VTATTFTDTRGFYAIIGVPTGIWSVSATKEGYDTVRYDGINVIAGNKTVQNFVLTKK"
     gene            8413..9882
                     /locus_tag="PLA17_01445"
     CDS             8413..9882
                     /locus_tag="PLA17_01445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPK84107.1"
                     /translation="MMKTKSALIYAALISTAVITVISCAVNPVTGRKQIMLMSEAQEI
                     QLGLSYDPQVLASFGEYNNPALQSWVQAKGTEMGKISHRPNLQYHVKVVDSPVVNAFA
                     VPGGYIYLTRGIMANLNNEAEFIGVLGHEMGHIAARHSVSQQTKQQLGTLILIGGMVA
                     SPKFANYAEQAMQGLGLLFLSFSREDEREADALGSEYATKMGYDGTKMADFYKVLIRM
                     NLSEAEGGVPTFLSTHPDPGDRYNAVLRNTKAWQDKLQLPSYKVNADSYLQMIDGMVY
                     GEDPKQGYTIDNIFYHPELRFRFSFPAGWQLTNAPTQVTIQPSDGKALMLFTLSNQKT
                     LQSAVDTTMAMYGLQLQRSEKKSVNGLQAIVTLAKQPVQDQSTGATGTNVVLTYFVQY
                     GSLIYVFHGVSTEADFESYAPTMEASMKTFAQLTDQSKINVKPKRIHVRKVQRTGTMA
                     SVLSSFNMPQNMMEELSLLNNIELNETVQAGRYIKIIGE"
     gene            10135..10269
                     /locus_tag="PLA17_01450"
     CDS             10135..10269
                     /locus_tag="PLA17_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84108.1"
                     /translation="MKTYYQITAEEIGIVLLRKRKIAKALRRWLRENGMPYEYVFYVR
                     "
     gene            complement(10586..11017)
                     /gene="mscL"
                     /locus_tag="PLA17_01455"
     CDS             complement(10586..11017)
                     /gene="mscL"
                     /locus_tag="PLA17_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPK84109.1"
                     /translation="MGIVKEFKEFAVKGNMLDMAVGIIIGGAFGKIITSLVNDVLMPP
                     LGLLLGKVDFSSLKAILQKGSDAVMDGAVVVQPAVSEVSINYGIFLNNIIDFLIVALC
                     IFFVIKGMNTLKKKEEAAPETPPPPSEEAVLLKEIRDLLKK"
     gene            11227..11427
                     /locus_tag="PLA17_01460"
     CDS             11227..11427
                     /locus_tag="PLA17_01460"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPK84110.1"
                     /translation="MAVLKVIEVLASSPVSWEDATASAVKMAAKTVREIRSVYVQDQS
                     AVVNKDKITEYRVNVKITFEVV"
     gene            11442..11903
                     /gene="lysM"
                     /locus_tag="PLA17_01465"
     CDS             11442..11903
                     /gene="lysM"
                     /locus_tag="PLA17_01465"
                     /inference="COORDINATES: protein motif:HMM:NF008399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HPK84111.1"
                     /translation="MGLISFIKNAGAKIFKSEEKREEEKANLITGHIKKFGFDVSKVK
                     VAVDDEKVILTGSVDTHQNKNKIIVTAGNVEGISVVEDHLLVITPEPVAPPEPEKQFY
                     TVKKGDYLSKIAKEVYGDARKYPIIFEANKPMLKDPDLIYPGQVLVIPPLK"
     gene            12053..12511
                     /locus_tag="PLA17_01470"
     CDS             12053..12511
                     /locus_tag="PLA17_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84112.1"
                     /translation="MRKIRIISPFISVFLITLILSGSFGFTLIHHTCLHCGTNETIAS
                     LCGDPVGNKCFGDRKATCCSHDDCDVQHRHSTGDMVLSDDCCTHEAERIVTDELVRTE
                     AQNEILPYFLAATIIAVMEEYQTNNYNHFSEEQPFHCGRDLTTMLCCIRS"
     gene            12732..14993
                     /locus_tag="PLA17_01475"
     CDS             12732..14993
                     /locus_tag="PLA17_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK84113.1"
                     /translation="MMKHLTILITILLIPAFVVAQGRLEGKVMVAGNDDAPDGEGLPG
                     ANVVWAGTTVGASANAAGYFTIKKVKDHNLLVASFVGYTTDTVVVDPDTDYIEIILEE
                     SSMLDEIRVIGRATGTHIDRKATIATVNITGAELCKAACCNLSESFVTNVSVDVNYAD
                     AATGAKQIQLLGLAGSYVQILTENIPSMYGLGAAYGLSYIPGPWMESIQVSKGTSSVR
                     NGNESITGQINVEYKKPVTSEILFVNGFVSDAGKIESNANASLRLNPYWSTMLLGHGE
                     TQSGASDHNKDGFRDEPDVRQYSFMNRWDYLSDNLTFRTGIKYMNEERIGGQFDYNRN
                     DPNTWDSGYGINMATERAEAFTKVGGMFGPDDNMSLGWIQSFAFHEMNAWLGYNKYDA
                     TQKSYYTNLLYQYFPGEKHTVDAGVSYRFDHYDELQGIYRPLTGHITTNINSETVESV
                     PGVFMQYTYTDSAKVTLLAGVRYDFYRNHGAQFTPRLHLRYEITPHITLRASAGKGYR
                     TVHVLAENMYFLSSSRQMVIADNLDIEEAWNAGINMTGYLHIGKRELRVTGEAYRTSF
                     VKQIVIDLDSDIDRVSFYNLDGKSYSNVYQLEAQIEPVTGLDITAAWRWNDVKQTIGG
                     ELREKPLASRYKGLLTASYTTRMRKWQVDCTLQYNGPGRIPSTMANPEPYRRADHFSA
                     YPVLNAQITKYFRKWSVYIGAENITGSRQHDAIIAANDPFGPYYDGSMIYGPVHGRKL
                     FAGFRFALDRPQN"
     gene            15111..15452
                     /locus_tag="PLA17_01480"
     CDS             15111..15452
                     /locus_tag="PLA17_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HPK84114.1"
                     /translation="MKTNLLITAALLFLFSSLTLSAQVKNKKNLSTVRFETSIDCENC
                     VNTIMKNIPFEKGVKDVKCDLETKEVTVQYQKDKTSPETLKRSIEKLGFTAKEVTEKK
                     KEEVSPQGSRQ"
     gene            15680..17233
                     /locus_tag="PLA17_01485"
     CDS             15680..17233
                     /locus_tag="PLA17_01485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPK84115.1"
                     /translation="MKTPRVITISFLFVLLILSGSCKNRAPVSTSLPRSTPEAEGVAS
                     AELITFLDSVAAGRHEFHSIMIIRHGKVVAEGWWAPYSRELRHTMYSTSKSFTSTAVG
                     FAVTEKLLSVDDTVISFFPDQLPDTVSPFLAMMTVKDLLTMSAGQVPDPTGRISTSSG
                     DWVKEFLATPVLKEPGTEFLYNSMATFMLSAIVQKVTGEKIADYLRPRLFEPLGIEGY
                     DWEESPGGITSGGWGLRVKTEDMAKFGLLYLQKGMWNGKQIIPAAWIEEATTFKIDQA
                     PGELPEEKAMSDWKQGYCYQFWRCRNNAYRADGAFGQYIIVMPEKDAVVAITCESPDL
                     QDEINLVWDYILPAMKEGPLQEDKETLTVLKERFANLALPVPVEKPDSPLASAISGKS
                     FKIISDHGSQGYFNFRFSGDTCHLSLEMNGEEASIAFGRGRWIESETGFPGPSLLSRP
                     LGFTSHRIAAAYEWKDDSTLVLTARYIESPHSIMFNFTFEADTLIMNMRLSPPPGLDT
                     PQVKAVMEE"
     gene            complement(17328..18059)
                     /locus_tag="PLA17_01490"
     CDS             complement(17328..18059)
                     /locus_tag="PLA17_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPK84116.1"
                     /translation="MEGWLARTEMLLGPEALKRLKESHVLVAGLGGVGSWAAEMICRA
                     GTGAMTIIDGDVVTAANRNRQLPALTSTTGRKKADVMGERLLDINPGLQLRIIDEFIR
                     DQRMVDILEAEKYDFVVDAIDTLSPKVFLIYHSLRLKLRVVSSMGAGGRFDPTVIRVS
                     DISETNFCNLARMLRKKLHKLGVYEGFTAVYSPEVVDKSLIVRGSDESNKASSVGTIS
                     YMPAAFGIACASVVIRELAGVTGAD"
     gene            complement(18059..18730)
                     /locus_tag="PLA17_01495"
     CDS             complement(18059..18730)
                     /locus_tag="PLA17_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPK84117.1"
                     /translation="MKPRGEIRYIDFHTHSQGGGSDAIAVRNLMAGDEVPADFPANTL
                     FSAGIHPWQLTGDNTEELQTELLLTAAHPHVVMIGEAGFDRLRGVQGEVQYRVFLFQA
                     HIAAEMGKPMVIHCVKGWDELRRARREVKPGRPWVIHGFRGSGSLAASLAGEGFWFSL
                     GAKGLTTEVLQSVHHEKLLLETDESGQSIAEVYRLFEAATGYDGQKAESLIRNNFRLL
                     MNYSL"
     gene            complement(18730..20250)
                     /gene="gltX"
                     /locus_tag="PLA17_01500"
     CDS             complement(18730..20250)
                     /gene="gltX"
                     /locus_tag="PLA17_01500"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPK84118.1"
                     /translation="MTKKNIRVRFAPSPTGPLHIGGVRTALYNYLFARHHGGTFILRI
                     EDTDQARYVDGAEEYIMDSLEWAGIKVDEGVREGGPFAPYRQSERKDIYHHYAGELLR
                     SGDAYYSFDTPEELERLRQEYESRGETFNYGCTTRMSMCNSLTLPAAETERRIASGEN
                     YVVRYRMPENEDIHFNDLIRGDIVVNSSTLDDKVLYKSDGMPTYHLANIVDDHLMEIS
                     HVIRGEEWLPSLPLHVMLYRSFGWEAPLFAHLPLLLKPTGKGKLSKRDGDKMGFPVFP
                     LYWPYGETATGYREEGYFPEAFVNMLALLGWNPGTEQEIFSMDELIEAFTIDRVGKSG
                     SRFDPDKAKWFNARYLQMKSNEELAGLFMPLVNQKGFSPDRAILVRIVSLVKERVGFV
                     KDLWNESDFFFVAPESYDPEVVRKRWNADSPGQMSELRAKVAEVQPFAPEVLEPSVKE
                     WIDSKGYKTGAILNLFRLLLVGASRGPHLFDIAALIGREETLRRLDDGIRRLSQLT"
     gene            complement(20348..21310)
                     /locus_tag="PLA17_01505"
     CDS             complement(20348..21310)
                     /locus_tag="PLA17_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPK84119.1"
                     /translation="MKNILVTGSVGQIGSELTLELRKRYGNGNVIAGINRTQPTGALA
                     ETGPFMKVDATNREELAAAVKKYNIDTIYHLAAVLSAVGEKNPGLAWNIGVNGLYNVL
                     EAAREFNCAVFFPSSIGAFGPTTPLDNTPQDTIQRPTSMYGITKVTGEMLCDYYHLRF
                     GVDTRGVRFPGIISNVTLPGGGTTDYAVEIYYEAVKHQRFTCPLSKGTFLDMMYMPDA
                     INAAINIMEADPSRLKHRNAFNVSAMSFDPEIIAAEIRKHIPGFIMEYDVDPIKQKIA
                     DSWPNYMDDSAAREEWGWNPVYNLESMTGDMLAVITEKHRKGLI"
     gene            21508..>22693
                     /gene="kbl"
                     /locus_tag="PLA17_01510"
     CDS             21508..>22693
                     /gene="kbl"
                     /locus_tag="PLA17_01510"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPK84120.1"
                     /translation="MYTNFQEFLTGELSAIKEAGLYKDERIIVSPQGAEITLNTGQKV
                     LNFCANNYLGLSANKKLVEAARQALDTHGYGMSSVRFICGTGDLHKELEQKLAHFFGT
                     EDTILYASCFDANGGVFEPLLTSDDAIISDSLNHASIIDGVRLCKAQRYRYANADMND
                     LEEKLKEAQAQRFRLIVTDGVFSMDGNVAPLDRIYELANKYNAMIMVDESHSAGVVGE
                     TGRGVTELYNLKGKIEIITGTLGKAFGGAIGGFTTGKKEIIDMLRQRSRPYLFSNSIP
                     PLVAAAGIKMVEMMTETNELQDKLHFNTKYFMEKMMKAGFDIKPTESAICAVMLYDAK
                     LSQEMAARLLREGIYVIGFYYPVVPKGEARIRVQLSAAHDTEHLDKAITAFIKVGKEL
                     NII"
BASE COUNT         6045 a         5746 c         5492 g         5410 t
ORIGIN      
        1 tcagtttcaa cccctcgcgc cggagactgc tgaaaaaccg cggaccaaca tcaataccga
       61 cggcataaac aaaaagtatc aggccgaaat cgcggacaaa ggtcagcaca tgcaggtcag
      121 ccctcactcc gaaatgtgcc agcaggagtc cggaaaacag cacgccggca accccaagcc
      181 tgagcttctt cacatttatt ctgccaagga gaatcccggt aaaggcagtg atgctcaggt
      241 aaagaatcgt agtagcgatc cccggtccgg caaacaggct tctaaatgcc tccatagaaa
      301 ttcttcacat tagtagtatc tggcgccctc tcattcaggc agggggcact gtcaatcaac
      361 ggccccaaat ttacaaacta tgcggaaatc aatgactgac tctcgtcata caatgttctc
      421 cggtaaaaga ggccgcgtca gacctgtaat cactatccta acaggctcag actcagtagt
      481 atgattggca acgttattac gctgaaaata gttgatacaa agacattggc tgttgccgtg
      541 ccgtcatcct cgcccagtat gctcaccatt attaccacgt tgaccatgca gggcatcgcc
      601 gcctgcatca gcatgacaga gaccacctcc ttgcgaatga tgcccggaaa cagaaggtcg
      661 aaaagaatga acaaacccag aaggataaat ggcaccagca gcaacctgtt gaatgagagc
      721 aggtaggcat ttctgttgcg gagcatcttc ctgccatcgg caaaatagat aaccgaagca
      781 atgaaaatca tagaaagcca ggtgttggtt tttccaagtg caccaaccga atcaaggata
      841 aactgcggga gcttcactga aaggagaaag agcaggaatc ctgcaataat tgctatcgta
      901 ttgatattga agattttttt cattcctgat ttcaggctga atgatcccgc cggggttaat
      961 ataccaaccc cgagcgtcca catcaggatg tttgagacca gcacgaacat actgccgtaa
     1021 agcactccct cctgcccgaa ctgggccgag atcacaggca accccaggta gattatgttg
     1081 ccaagcatac tgtgaagcct gaatatcgaa ccatggctcc cttttattcc gaagatcacg
     1141 gtagtcagcc atccggcgaa gagcataaaa aggatgacca gcactgcaag cagcaatgcc
     1201 tgcaggctgt tggccaggag cctcggagtg acctcgatgg cgctgaagct ggtaaagagc
     1261 atggatggaa gcgtcacata gaagatcagc ttcgcaagga aatccttcgt gccggcagtg
     1321 atcacccctg cacggcaggc aatgagcccg ataacaacta tggctgtcag tatgcctatc
     1381 tgtcggagta tgataacgga agtcattaaa atcccttatg atgttacatt aaacggtggt
     1441 cttcaaaaaa accacagcaa agatagctac cggaagagaa aacggcagag ttcccgtcat
     1501 actttctcac tgaaatagtt ttactaattt tgggaagcag gattacatta ataaaacacc
     1561 aggttcaaca agagagacca aaatcaccgg taccccatga agatactaat aattgaagac
     1621 gaatcgaggg tagcctcttt cataaaggaa ggcctggagg agaaatcgta taaaacggtt
     1681 gtggcatacg atgggatcac gggagaggag gctgcaatga aagatgatat cagccttgtc
     1741 atccttgacc ttatcatccc cggcaagagc ggcattgacg tctgccggtc gattaaggca
     1801 gtccggcctg accttcccat tctgatgctt acggcgctca gcactaccgt cgacaaggtc
     1861 aggggctttg aggcaggggc agatgattac ctggttaagc cttttgactt cccggagctc
     1921 cttgcacgga tcaaatcact gctcaagaga agcaggaatt caacgcacca gacgcaatcg
     1981 ctccgggtgg ctgacctgga gcttgacctt atgaggaagg tagcaatccg cggtggcaaa
     2041 gtcattgagc ttaccgccaa ggaattcgca ctgcttgaat acttcatgcg caacagcgga
     2101 agggtcctct cacgcgctga catatcggaa aaggtgtgga acataaccta tgacaccgga
     2161 accaatatcg tggaggttta tgtaaatatc ctcaggaaga agatagacag ggacttcgag
     2221 ccgaagctca tccacacacg gataggattg ggatacattt tcacagacga atagatgaag
     2281 atacggctga aaattactct tcagtttttt ctgatggcca gcgtaatatt gctgctcttc
     2341 tcactgacag tctatatagt gtcagccacc taccgtcgtg atgatttcta ccagcggctt
     2401 gagagcaggg ccatcagcac ggccaggctg ctgattgagg tggatgagat tggtaccgac
     2461 cttttgaaca agatcgaaga ggataacccg gtctcactgc ccgatgagaa gatcataatc
     2521 ttcaactaca agaacgaaat tgtttattcc actgatacca ggaacgttat tctgtatgac
     2581 agcgatctgc tgacgcggat cagggcatcg ggacagataa agacgaagag cggcaggtat
     2641 gagctgatgg gtatcctttt tacggaagag tttaatcgtt ttgtggttct cagtgcggcc
     2701 cgtgacatat acggatttga caagatgaag aatctgagga caacactgat acttctcacc
     2761 ggtatttttc tattgctgtt ttaccccgcc ggatggcttt tcgcggcacg gtcactctct
     2821 ccgatatcga aagtggttga ccgcgtggag gagatatcca ttgccagtct tgacctgagg
     2881 gttgatgagg gcaacgggaa ggatgaactg gctcatctgg cacacacctt caaccgcatg
     2941 cttgaaaggc ttgagacagc ctttgaggtg cagaaggatt tcatttcaaa cgcctctcat
     3001 gaactgcgga caccgctgac agccataagg gggcagcttg aagtgcttct tatgaaggag
     3061 cggacagaca aggaatacag ggattcgact gctgccgtac ttgaggatat cagcaggctg
     3121 aaccagctgc ttgaccggct tctgctgatg gcccacgcca gctccgaggg ggccagggag
     3181 ggtttcaggg aggtgcgaat tgatgagatt ctgtggcagg cacgcgagga actgctgaaa
     3241 cacgaaaagg aatatacggt aactgttgcc ctcgacgaga atatctcaga cattgaaaac
     3301 cttaccatga gaggcgatga gcagctactg atctcagcag taaccaacgt catggagaac
     3361 ggctgcaagt attcccctga caagtctgta agggttcttc tgtcacatga cgcggataac
     3421 atattcgtaa gcttcacaga taacggtccc ggcattgcag aggatgagat tgagcgtgtt
     3481 ttcgaatctt tttaccgcag caaagccacc gcatcggtaa ggggacacgg gatagggctc
     3541 tccctggcca gcaagatcat cgacatccac aacggaacga taaccgtaaa atcgggtcag
     3601 ggtgagggaa ccaccatcag gctcgcattt cctgccgaac gttctgtcgc tgtttaaccg
     3661 ggacacaatc tgaaatccgt taactcagga accggatgcc ctgtatcaga aagttccgtg
     3721 aatgccacaa tttatctcct tagaaagttc cggcgatagc aatatgtgtc tccttaagat
     3781 tatcaggccg gactataacc agacggatga ctgatggaga ccatcgtcct ctgaaacatt
     3841 gcttaattca ttgattgaca cgccctgatc tttaccttca ggcagcagga ttgcttttgc
     3901 gtgcgtgaaa ataagtgaaa ccgtttattt cggtcatgcc taccagctca agccctgact
     3961 ccttcagcag gttggcaacg gtcctcctgg tcaggtacct gttcgtcctg aggtcaatga
     4021 tgctgtacat cctgttgata aagggaatcc ttgactcaat accgttctcg taggcatcgg
     4081 ccttgtcaga tgcatgccct gaagaaccgg catttttgtc agacccggcg ccatgtgacc
     4141 tgttgtcaat aaaacagccg atgaaattac tttttgcagg gagaaggccg gtaatctgcc
     4201 tcaggaagtc acgtatctcc ctgatatagt tcagttgcct gaggttgatg actgtcctga
     4261 tgcccttcat atcttcggca tcgtaaaaat agtgacgcga ggaaggaatg acaagcatac
     4321 ctgatccctt caccaggccc ttgcggtcaa ggtagtctat caggccctga cgcacctcga
     4381 ggtcaagctc gtcgaacagg tgcatcagcc cgctgtccct taacgacagc ttgcgcgggc
     4441 tcttccttac tacggcctga tccctggttt tttccttatt caaacctatg gttcctttat
     4501 tcatgtcctc gccagatgcc ttttcttata agcaattttt atgccacggt gttttggcag
     4561 catcccgtaa attggatttt catcccgaag atattcactc ttcactatat gaaccaaact
     4621 attttgatat ttgaacccaa cccgttgacc aacggagcca aactggggat aatcagcata
     4681 aagctgatcc gttactttca gcctgtcaat aagttatttc agtgtaatta gctctatatc
     4741 ttatagttta gtacgttttc agatcatttt cagcgttcaa ttctgaactc cctgtagcct
     4801 gccagtggcc cttctgtcac cctggcattg tcaacgagcc aggttccggg gccatgtctg
     4861 caccatgccg caaactcctc cagaggttcc ctggctcctt ctgcctctat aaccacgctt
     4921 ccgtcaggta tgttcttaac atagccggtg attccccgct tccatgcctc aatagcgacg
     4981 ctccagcgga aacccacacc ctgcaccatt ccggtgacat gaatcctgta atgaacctta
     5041 agtcccatgc ctcatcggag tttgtcatgg agcgaatgaa ccactgttct gtttttacct
     5101 acaatttaag ttaaatttgc acattttgaa aatgttttgt caaataaaaa atagctgaat
     5161 ccgctgatga aaaaaggatg gaaaatagtt atcgttgtac tcgggataat catattcatt
     5221 ccgctgtttt tcatcagctc cgttcagatc ggagtcttcg gccgcctgcc cgggaagaag
     5281 gaacttatca gtttccgtaa tgccacagct tcagttgtgc tttcgcacga aggagagaca
     5341 attggcaggt tcttttcaga gaaccgtatc accatcccct tcgaccagat gcctgctcat
     5401 ttgatcaact cgctggtggc caccgaggat gcccgcttct atgagcattc cgggatagac
     5461 tcaaggagtc tggtcagggt ggttttcaag tctatcctgt tcagtgaccg cagttccggc
     5521 ggagggagca ccataaccca gcaactggca aaaaatatgt atggacggaa ggattaccgg
     5581 gtgttttccg ttatcctgaa caagaccagg gaggttatcc tcgcgaagcg gattgaaaag
     5641 gtttttacaa aggacaagat acttgagctt tacctgaaca ctgtctcgtt cggcgagaac
     5701 atttacggaa ttgaagcagc ctcgcagcgt tatttcagca aaaaggcggc tgacctgaaa
     5761 gtggaggagg cggccctgct caccggtatg ctcaaggcca ataccatgta caatccgcgt
     5821 ctctatcctc agaatgcagt caggagacgc aatgtggtgc tggggcagat ggagaaatac
     5881 ggttatctgg agggcagggc cgccgattcg ctcagcgcac tgcccattaa gcttgactac
     5941 aacaagacgg atttatcagg gcctgctgat tacttcatgg tcagggtaag gattgagaca
     6001 gatcgtattc tgaatgagat atcatctttc cggggaaagg agtgggatat tgagcgtgac
     6061 ggccttatca tcaccaccac cctgaggatg ccgctgcagc aggctgccgg tgaggcattt
     6121 gccgcccata tgcccaggat gcagaagagg ctcaatgagc agtacagcac atcctccgga
     6181 cgccgggccc tgaaagggat acctgacagc ctgaagaaga tgatgacaac acttcacgcg
     6241 ggcctcctgg ccatggaccc ggctacggga gccataatgg catggaacgg cggaattgac
     6301 tttaaaacac agccctatga tcagattctg gccaggcggc aactcgcttc agtcttcaaa
     6361 ccgttcatat tcgctgctgc acttgaagac ggggtggaac catgcaggta tcttgacaac
     6421 gactctgtca ccctgtcagg attcaacgac tggagtcctg aaaactataa ccactcatac
     6481 ggtggcaagt attccctggc aggagcactg gcacagagta tgaacatacc caccttcagc
     6541 ctcttcctta tgatagggtt cgataaggtt gacaggctgt ggcaggagat gggattctcc
     6601 ttccctctgg tgaacatgcc gtcactggca atgggtactg cggaggccag catactggag
     6661 gttgccaggg catatgccgc cttcgccaac ggcgggatgc ttgtcactcc gtatcttatc
     6721 gaatcggtga aggcgcctga cggcagaata ctctggcagt acatgccacc acgccgatca
     6781 gaccgggttc tttccgaacg ttcagccaca ctcatggctg ccatgctcga aaaggccatc
     6841 agggaaggaa caggagcctc tgttcacagt gcctacggtg tgaaggtacc gctggctggc
     6901 aagacaggca cctcacagaa ttatgctgat gcctggttcg ctgctttcaa cccggcgatg
     6961 gttattgtaa gccgtgtggg cgcttcaaca ccttcaatac atttcaacag cggctcctac
     7021 ggttcgggaa gcgccctggc cctgccgctg gtggctttga ctcttaaaaa ggtggagaag
     7081 aataaagaaa taatgagtgt gatcaacacc cctttcgctc ccctgccccc ggaactggag
     7141 atggcgcttg actgccccga cttcagggag aagagcttct tcgacaggtt gtttgacatc
     7201 ttcagggaca ataatattga ctatcagaaa gagacccggc ggaggcgaat cttccgcggc
     7261 cggattttca gaatctgaaa ttcatcattc cttcattata catatcctac ttttgcatga
     7321 gtagttaaaa ccattaaaca gaagcaagat gaaacctgca tgtgtacaaa cacctgatta
     7381 tctgattttc agctatgtgg gttccatcat acctttaaaa ttaaagaatt gaattatgat
     7441 gaaaaagaac gcaatcctgc tgttagcagc ggcattagtc cttatctcag gagcatgcaa
     7501 cgaaataagg aacggagatg gaacaggacg agtgattctg aaggttactg acgccccgtt
     7561 tgacatcaac agtatcgaat cagcacttgt gaccatcaca aaagtagagc tgagagaaga
     7621 atgtgacggc atcagcgacg catgtgagtt tatcacactt tcggaagata ccgtgaccct
     7681 tgacctgatc gatctcagga atggtgttac cgaaacactc cttgatatgg agattccggc
     7741 cgggaggtat gatatggtgc gcctctacat ttacgaagcc ggcctgaagc tgaaagactc
     7801 cgaattaatt caccgcttga aagtaccagg tggcagccag tcaggaatta aggttttcct
     7861 tcgcccggtt cttgaagtgg ctgagggaag ccttgaggaa atcctgcttg atatagacct
     7921 ctccaggtca tttatcctca ggggtaaccc cgctcacaat aacggcttta tcttcaagcc
     7981 tgtggtaagg gcagccaata tggcgaaggc tggttgtata agaggagtgg ttactgatac
     8041 tgaaggaacg gagataagcg gggcaagtgt ctggattgag caggacaccg ttactgccac
     8101 aacattcact gatacccggg gcttttatgc gattatcggt gtgccaaccg gcatatggtc
     8161 agtttcagca accaaagaag gctatgatac ggtccgttat gacggcataa atgtcatcgc
     8221 aggtaataaa actgttcaga atttcgtact taccaaaaaa taacaggctg aatagtttta
     8281 atatggctta aatttgagcc gtgccggaga ggcacggctt ttgattttgt ataattaata
     8341 tctagatttg aaagattgca gccatgttta tggcgatgaa ccaaatcctt gcaaagaaac
     8401 catttacctg atatgatgaa aacaaaaagt gcacttatct atgcagcgct tatcagcaca
     8461 gcagtgatta cggttatatc ctgcgcagtc aacccggtta ccggcaggaa gcagattatg
     8521 cttatgtcag aagcacagga gattcagctt gggttatcat atgacccaca ggtacttgcc
     8581 agtttcggtg aatacaataa tccggcactt cagtcgtggg tacaggcaaa ggggacagag
     8641 atgggaaaga tttcacaccg gccgaacctc caatatcatg tcaaagtggt tgactcgccg
     8701 gtggtaaacg cttttgcggt tccaggaggt tacatatacc ttaccagagg tataatggca
     8761 aatctcaata acgaagctga attcattggt gttctcggcc atgagatggg ccacattgcc
     8821 gcccgccact cagtcagcca gcaaaccaag cagcagttgg gaacgcttat tctgataggc
     8881 ggaatggtag cctcgccaaa gtttgccaac tatgccgagc aggcaatgca gggtcttggg
     8941 ctactcttcc tgagcttcag ccgcgaggat gaacgtgaag ctgacgccct cggatcagaa
     9001 tatgccacaa aaatgggata tgacggtacc aaaatggccg atttctacaa ggtgcttatc
     9061 agaatgaacc tctctgaagc tgaaggcggg gttcccacgt tcctgtcaac ccaccctgat
     9121 ccgggtgaca ggtacaatgc tgtgctcagg aacaccaaag catggcagga caaactgcag
     9181 cttccctcct acaaggtgaa tgcagattca tatctccaga tgattgacgg gatggtctat
     9241 ggcgaggacc cgaagcaggg atacactatc gacaacatct tctatcaccc cgaactcagg
     9301 ttcaggttct cattccctgc cggatggcaa ctgacaaatg cacctaccca ggtaaccatt
     9361 cagccttctg acggaaaggc actgatgctc ttcaccctct ccaaccagaa aacccttcag
     9421 tcagctgttg acaccacgat ggcaatgtac gggctccagc tgcagcgcag cgagaaaaag
     9481 agtgtcaacg gactgcaggc tatcgtcacc cttgccaaac agccggttca ggaccagtcg
     9541 accggagcaa cgggaacaaa tgtcgtcctc acctatttcg ttcagtatgg atcattgata
     9601 tatgtattcc atggtgtctc caccgaagct gacttcgaaa gctatgcccc cacaatggaa
     9661 gcatcgatga agaccttcgc acagctcact gaccagtcaa agatcaatgt caagccgaaa
     9721 agaatccatg tcagaaaggt ccagcgtaca gggaccatgg ccagcgtact cagctccttc
     9781 aacatgcccc agaacatgat ggaagagctt tctctcctca acaacataga gcttaatgag
     9841 acggtgcagg caggcaggta catcaagatt atcggtgaat aggcgcagac acagaactga
     9901 cattggcata taattaagtg attccccggg ccaggtttcg ggccgaggtg cgcgccgggg
     9961 cgcggacagg ggccgctgtg ttttccttca cccctgcccc aagagaacaa tcgatcaaaa
    10021 ccagttatca tgacagaaga cctgttggtc aatacctgtc cattgtccgt caaaagagac
    10081 tggacaaaag ctcttttcga tcgtatatta gtatagaatt caaccacgga aaagatgaaa
    10141 acgtactatc agataacagc agaagaaata ggtattgtac tgctccggaa aagaaaaatt
    10201 gccaaggcac ttcgcaggtg gttacgcgaa aatggaatgc cctacgaata cgttttttac
    10261 gtcaggtaat atctcacgat aaggttctgt ttaatggttt ttccggtgta atgagaccga
    10321 cccgtcaagg gtcggttttt ttatatcaat gctttaatga ctgctgttca ctgagatgac
    10381 tgcctgctgc tgacggcaga cagccaactg cagaaaaggc cgcactcttg cgaatgcagc
    10441 ctctgttcca ggtctattgc ggtgagccgc tctaacccct gtcaggaaag aacctgtgcc
    10501 accagaaaaa tatccttatt cttgccatag ttaaatcgcc tgcaaatatg caaacaatta
    10561 ttgacagcaa ttattgtacc actaactatt ttttcagcag gtcacgtatc tccttcagaa
    10621 gaactgcctc ttcactcggt ggaggaggag tttcaggagc tgcctcttcc ttcttcttca
    10681 gggtgttcat tccttttatc acgaagaaga tacaaagcgc cacaatcaga aagtcaatga
    10741 tattgttgag gaatatccca tagttgattg acacttcgct gactgcaggc tgtacgacta
    10801 cagctccgtc catcacggca tcggagcctt tctgaagtat tgctttaagg ctgctgaagt
    10861 ccacctttcc caacagcaag cccagtggcg gcataagaac gtcatttaca agtgatgtga
    10921 taatctttcc gaatgctccg ccgataatga taccaacagc catgtcaagc atgttgccct
    10981 tcacggcaaa ctccttgaat tcctttacta ttcccatact ctttcaggtt tttggtttgt
    11041 taatacaaac aggttaactg atatcaaata tacaaaaagg caggagatat tcagtttaac
    11101 ggtgactttg atttaccact ccgtgattca ttaaacccga ttattttaat taacttacga
    11161 agcttaccgt ccaaatcaga cggaattgtc aacatattaa atattaacct tttaaaaaga
    11221 atgactatgg cagtacttaa agttatcgag gttcttgcat catcaccggt cagctgggag
    11281 gatgcaacag cctctgccgt gaagatggca gcaaaaacag tccgggaaat acgttctgta
    11341 tatgtacagg accagagcgc tgtggtaaac aaggacaaga tcacagaata ccgtgttaat
    11401 gtaaaaatta cctttgaagt agtttaatta aaaacaggat tatgggactt atttcattta
    11461 tcaagaatgc aggtgcaaag atcttcaaat cagaagagaa gcgcgaagag gaaaaggcaa
    11521 atctgatcac cgggcatatc aaaaaatttg gttttgacgt aagcaaggtc aaggttgctg
    11581 ttgatgacga gaaggtgatt cttaccggat ctgttgacac ccaccagaac aagaacaaga
    11641 ttatcgttac cgcaggtaac gttgaaggca tctcggtggt tgaagatcat ctcctggtca
    11701 taacacccga acctgttgct cctcccgagc cggagaaaca gttctacacg gtcaagaaag
    11761 gtgattacct ctcaaagatc gccaaggagg tttatggtga cgccaggaaa tacccgatca
    11821 tcttcgaagc caacaaaccc atgctcaagg atcctgatct gatctatccc gggcaggttc
    11881 tggttatccc tccactcaaa taggaaagtt ttgcattcga tatgtgcaga gggtgcctca
    11941 aaagggcgcc ctttttcttt tggcttactg ttcatctctg aggcaggtca taagcctgcc
    12001 tcagaaattc tcgtcataat atttgtttat aaatgttgtt atatttgggc cagtgagaaa
    12061 gatacgcatc atatcgccat tcatctccgt cttcctgatt acactcattc tttcaggcag
    12121 cttcggtttt acccttattc atcatacctg tctccactgc gggaccaacg agacaatcgc
    12181 ctccctctgc ggtgacccgg tcgggaacaa gtgcttcggt gaccggaaag caacctgctg
    12241 cagtcatgat gattgtgacg tacaacaccg ccacagcacc ggtgacatgg tactgtcaga
    12301 tgactgctgc acacacgagg cagagaggat cgtgacggat gaactggtgc gtacagaggc
    12361 acagaatgag attcttcctt acttccttgc agcaacgatc attgctgtca tggaagaata
    12421 tcaaaccaat aactacaatc atttttcaga agagcagccg ttccactgcg gacgcgacct
    12481 gactacaatg ctctgctgta tccgctcctg atgctttcat agggaaacaa ctgacaccgg
    12541 aaaggatgac tcctttaccc ggcctttgtt gttgacgctg catgcgtgat ggttttcaaa
    12601 aaccactgca cagatgccat ttaattaatt ttcaaaatat tccctatgaa acccacatgt
    12661 ttcaaacaga cagacacctg cttatatgat catcagtcat gtgggttcct cataccttta
    12721 aaatcaagat tatgatgaag catttaacaa tactgataac tattcttctc atccctgcct
    12781 tcgttgtggc ccagggtcgt ctggagggca aggtcatggt cgccggcaat gacgatgcgc
    12841 ctgacggaga aggccttccc ggtgcaaacg tagtctgggc cggaacaacc gttggcgctt
    12901 ccgccaatgc agccggctac tttaccatca aaaaagtaaa ggatcacaat ctgcttgtag
    12961 ccagtttcgt cggttataca actgacaccg ttgtggttga tccggacact gattacattg
    13021 agatcatcct tgaggaaagc agcatgcttg atgagatcag ggtaattggc cgggccacgg
    13081 gtacccacat tgaccgcaag gccaccattg ccacagtaaa catcaccggc gcggagctat
    13141 gcaaagcggc atgctgcaac ctctcggaaa gttttgtcac aaacgtctcc gttgatgtca
    13201 actatgctga tgccgccacc ggtgcgaaac agatacagct tcttggcctt gccggctcat
    13261 acgttcagat acttactgag aacatcccgt caatgtacgg gctgggagcg gcctacggac
    13321 tgagctacat ccccggcccg tggatggaat caatacaggt ctccaaaggc acctcctcgg
    13381 tgcgaaacgg aaatgagtca atcaccgggc agattaacgt cgaatacaag aaaccggtaa
    13441 caagtgagat actgtttgtc aatggattcg tgagtgatgc aggcaagata gaaagcaatg
    13501 ccaatgcttc actcaggctt aatccatact ggagcacgat gctgcttggt cacggcgaaa
    13561 cacagtcagg cgccagcgac cataacaaag acggcttccg cgatgagccc gacgtcaggc
    13621 aatacagttt catgaaccgg tgggactacc tcagcgataa tctcaccttc cgcacaggca
    13681 tcaagtacat gaatgaggag agaatcggcg gccagtttga ctataaccga aacgatccaa
    13741 acacctggga cagcggctac gggataaaca tggctacaga gagggctgaa gccttcacca
    13801 aggtgggcgg catgttcggc cctgatgaca atatgtcgtt gggctggatc cagagtttcg
    13861 ccttccatga gatgaacgcc tggctgggat acaacaagta cgatgcaacc cagaagagct
    13921 actacaccaa ccttctctac cagtacttcc cgggggagaa acacactgtt gacgcaggcg
    13981 tcagctaccg cttcgaccat tatgacgagc tgcagggcat atacaggccg ctgaccggac
    14041 atatcacaac caacatcaat tcggaaacag tagaatcagt gccgggagta ttcatgcagt
    14101 acacttacac tgattctgca aaagtgacac tactggccgg agtgaggtac gacttctacc
    14161 gtaaccacgg tgcacagttc accccccgcc ttcacctgag atatgaaatc acaccccata
    14221 taaccctcag ggcttcagca ggaaaaggtt accgtacagt ccacgtcctt gctgaaaaca
    14281 tgtacttcct ctcaagcagc aggcagatgg taatagcaga caatcttgac atcgaagagg
    14341 catggaacgc gggcatcaac atgacagggt atctccatat cggcaaacgg gaactcaggg
    14401 ttaccggcga agcctaccgt accagcttcg taaagcagat agtgattgat ctcgactccg
    14461 atattgacag ggtatcattc tacaacctcg acggaaaatc atactcgaat gtttaccagc
    14521 ttgaagcgca gatagaaccg gtgacagggc tggatatcac agccgcatgg cgctggaacg
    14581 atgtcaaaca gactatcgga ggtgagcttc gcgagaagcc tcttgcctca cgctacaagg
    14641 gacttctcac cgcctcatac acaacacgca tgaggaagtg gcaggttgac tgcacactgc
    14701 agtacaacgg ccccggcagg ataccctcca ctatggcaaa tccggagcct taccggagag
    14761 ctgatcattt cagcgcctac ccggtattga atgcccagat aacgaagtat ttcagaaaat
    14821 ggagcgtcta tatcggcgcc gagaacatca ccgggtcaag gcagcatgat gcgataatcg
    14881 cagccaatga tccatttggc ccatattatg acggttcaat gatctacggt ccggtgcacg
    14941 gccggaaact atttgccggc ttcagattcg cacttgacag accgcagaat taataacaat
    15001 caaaaaaaca acacgatgga gcccacatgt accaaattca aaaacacctg tttgtagaat
    15061 tttcagtcat gcgggttcca taatacctta aaaatcaaaa ttatattata atgaaaacca
    15121 atcttttaat cacagcagct cttctcttcc tcttctcctc actcactctc agcgcccagg
    15181 tgaagaacaa aaagaatctc tcaacggtca ggttcgagac aagcattgat tgtgagaact
    15241 gtgtcaacac tatcatgaag aacatcccct ttgaaaaggg cgtaaaggac gtgaaatgcg
    15301 atctcgaaac aaaagaggtc accgtgcagt accagaagga caagacctct ccggaaacac
    15361 tgaagagatc aattgaaaag cttggtttca ccgcgaaaga ggtgactgaa aagaaaaagg
    15421 aggaagtgtc cccgcaggga agcaggcagt aaggttgccg ggaaccggat tattgttata
    15481 cttttggata ttggtatcca aacctctgtt gccatataac ggggcggttg gtttttgctg
    15541 ccaaaaatat tggcaattaa tttgagtgct catttcagga aagatgatca actctgataa
    15601 tgaggtgatt atgaataatt atttttgacc acattgccat tttaaactat accaaactat
    15661 taactataat cacagttcca tgaaaacacc gagagttatt accatcagtt tcctctttgt
    15721 tcttctgata ctgtcaggca gctgtaaaaa cagagcgccg gtatccacgt cactgccccg
    15781 aagcacccct gaggctgaag gagtggcatc agcagaactg ataacctttc ttgattctgt
    15841 cgctgcaggc aggcatgaat ttcacagtat aatgatcatc cgccacggaa aggttgttgc
    15901 cgaggggtgg tgggccccct acagccgtga actgaggcat accatgtact ccaccagcaa
    15961 gagcttcacc tccacggcag tgggatttgc cgttacggaa aaactgctga gtgttgatga
    16021 cactgtgata tcattctttc cggaccagtt gcctgatacc gtctctccct tcctggccat
    16081 gatgacggta aaggacctgc tgacaatgtc ggccggacag gtacccgatc ctacagggag
    16141 aatctctacc tcatccggtg actgggtgaa ggaattcctg gccacgccgg tgcttaaaga
    16201 gccgggtacg gagttccttt ataactcaat ggccacattc atgctctcgg caatcgtaca
    16261 gaaggttaca ggtgagaaga tagctgacta tctccgtcca cgtctctttg agccactggg
    16321 aattgaggga tatgactggg aagagagtcc tggcggaatt acctccggcg gatggggact
    16381 gagggtgaaa actgaggaca tggccaaatt cggccttctg tatcttcaga aagggatgtg
    16441 gaacggtaag cagataatcc cggcagcatg gattgaggaa gccaccacct tcaagattga
    16501 ccaggcgccg ggcgagctgc ccgaagaaaa ggcaatgagc gactggaagc agggatactg
    16561 ctaccagttc tggcgctgcc gcaacaacgc ctacagagct gacggagcct tcgggcaata
    16621 tattattgta atgcctgaaa aggatgccgt tgtagccatc acatgtgaat cacctgactt
    16681 gcaggatgaa attaacctgg tatgggatta tatacttcct gccatgaaag agggcccgct
    16741 gcaagaggat aaagagacgc tgacagtgct gaaggagcgg ttcgcgaacc ttgcactgcc
    16801 ggttcctgta gaaaaaccgg actcgccgct tgcttccgca atctcaggca agagtttcaa
    16861 aataataagt gatcatggca gccagggata tttcaacttc cgcttcagcg gtgacacatg
    16921 ccacctctca ctggagatga atggagagga agcttcaata gctttcggga gaggcagatg
    16981 gatcgaatct gagaccggat ttccaggacc aagcctcctc agccggccgc tcgggttcac
    17041 ttcacatcgg attgcagcag cttacgaatg gaaagacgac agcaccctcg tgttgactgc
    17101 caggtatatc gagagccctc acagtatcat gtttaacttc acctttgaag ctgacaccct
    17161 gatcatgaat atgaggctca gtcctccccc gggcctggac acaccgcagg taaaggcagt
    17221 aatggaagaa taggatgctt cccgtggccg gacggagatc agtctgatct gccccggtta
    17281 ccaaatgata tccccgtatc agccgatgac ttgatacgga gagccgttta atctgcaccg
    17341 gttacaccgg cgagttccct gatgaccacc gaggcacagg caatgccgaa cgcagcgggc
    17401 atatatgaga tggttcctac agaggatgcc ttgttgctct cgtcagagcc cctgacaatg
    17461 agactcttgt caactacctc cgggctgtaa acagcagtga acccctcata cacacccagc
    17521 ttgtgcagct tcttgcgaag catgcgtgcc aggttgcaga aattcgtttc agagatatca
    17581 gacaccctta tgaccgttgg atcaaatctg cctccggcac ccatagagct caccaccctc
    17641 agcttcagtc tcaaggaatg atatatgagg aaaaccttgg gcgagagggt gtctatggcg
    17701 tcaaccacaa agtcatattt ctctgcttcc agaatgtcaa ccatcctctg gtcacgaata
    17761 aactcgtcaa taatccggag ctgcaggccg gggttgatgt caagcagcct ctctcccata
    17821 acatcggctt tctttctgcc cgtggtagat gtaagcgccg gcagctgcct gttcctgttt
    17881 gctgccgtaa ccacatcgcc atcaatgatt gtcatggcgc ctgtccccgc cctgcagatc
    17941 atctctgcgg cccatgatcc cacacctccc agccctgcca ccagcacatg cgactccttc
    18001 agtctcttca gtgcctcagg gcccagcagc atctccgtgc gtgcaagcca accttccatt
    18061 acaggctgta attcattaat aatctgaaat tattccgtat caggctttcc gccttctgcc
    18121 cgtcataccc cgtggcagct tcaaagaggc ggtacacctc agctattgac tgacctgatt
    18181 catcagtctc aagaagcagt ttttcatggt gaaccgactg aagcacttca gttgtcaatc
    18241 cctttgctcc cagtgaaaac cagaatccct cccctgccag tgatgctgcc aggctgccgc
    18301 tgccccggaa tccgtgtatg acccatggcc ggccgggttt gacctccctg cgcgcacgcc
    18361 tcagttcatc ccacccctta acgcaatgga ttaccatcgg cttccccatc tcagcggcta
    18421 tgtgtgcctg gaaaagaaaa acacggtatt gcacctcccc ctgcactcca cgaagccggt
    18481 caaacccggc ctcaccaatc atcaccacat gggggtgcgc tgcagttaac agcaactctg
    18541 tctgtaactc ttcggtattg tcaccggtca gctgccacgg atggatgcct gcagaaaaga
    18601 gtgtgtttgc gggaaaatcc gcaggcacct catcacctgc catcaggttc ctgacagcaa
    18661 tggcgtcact tccgcccccc tgtgaatggg tgtgaaagtc aatatatctt atctccccgc
    18721 ggggtttcat caggttaatt gtgaaagccg cctgatgccg tcatcaagcc gtcgcagcgt
    18781 ctcctccctt cctatcagtg cagcaatatc aaaaaggtgc gggcccctgg aagcgcccac
    18841 cagcagcagc ctgaagagat tgagtatcgc ccctgtcttg tatcccttcg agtcaatcca
    18901 ctccttcacg ctcggctcca gcacctccgg tgcgaatggc tgtacttccg caaccttcgc
    18961 gcgaagctcg ctcatctgcc ccggcgagtc agcattccag cgcttcctga caacctcggg
    19021 atcatagctc tccggagcca cgaagaagaa atccgactca ttccatagat cctttacaaa
    19081 cccgactctc tccttcacca gtgatactat cctgacgaga atagcccggt cagggctgaa
    19141 ccccttctga ttcaccagag gcatgaagag gccagcaagt tcctcattgc ttttcatctg
    19201 gagataacgg gcattgaacc acttcgcctt gtcagggtca aagcgcgaac ctgactttcc
    19261 gaccctgtca atcgtgaagg cctcaatcag ctcatccatc gagaatatct cctgttctgt
    19321 tcccggattc catcccagga gggcgagcat gttgacaaat gcttcaggga agtagccctc
    19381 ctcacggtat ccggtagccg tttcgccgta tggccagtag agtggaaata ccgggaaccc
    19441 cattttgtcg ccgtcacgct tgctcagctt acccttgcct gtaggcttga gcaacagcgg
    19501 caggtgcgcg aagaggggag cttcccagcc gaagctgcgg taaagcatca cgtgcagcgg
    19561 cagcgacgga agccactcct cgcccctgat cacgtgcgag atctccatca gatgatcatc
    19621 aacgatgttg gcaaggtgat aggttggcat accatcagac ttgtacagca ccttgtcatc
    19681 gagcgtggag ctgttgacca cgatgtcacc cctgatcagg tcattgaagt gaatgtcctc
    19741 attttcaggc atacggtatc ttaccacata attctcccct gaggcgatgc gtcgttctgt
    19801 ctccgctgca gggagggtaa gggaattgca catgctcatc ctggtggtgc agccatagtt
    19861 gaacgtctca ccgcgtgatt catattcctg cctgagcctc tcaagctctt caggagtgtc
    19921 gaaagagtag taggcgtcac cgcttctgag aagctcaccg gcatagtggt ggtagatatc
    19981 ctttctttcg ctctgcctgt agggtgcgaa ggggccgcct tcgcgaacac cctcatcgac
    20041 ctttatacca gcccactcca gtgagtccat tatatactcc tccgccccgt caacgtagcg
    20101 tgcctggtcg gtgtcttcta tccggaggat gaaggtcccg ccatgatgcc ttgcaaagag
    20161 atagttataa agtgccgtgc gcacgcctcc tatatggaga ggtccggtag gactaggggc
    20221 aaagcgtacg cgaatgtttt ttttcgtcat tatggtctgc tggttaattg ccggcgataa
    20281 agatataaaa ttaaaaaaga ggctgtcgcg gatgacagcc tcctgaacga taaaccattg
    20341 tttctgatca gatcagccct tttctgtgct tttcggttat caccgcaagc atgtccccgg
    20401 tcatcgactc aaggttgtaa accgggttcc atccccactc ctcacgggct gcagaatcat
    20461 ccatgtagtt tggccatgag tcagcaatct tctgctttat ggggtcaaca tcatattcca
    20521 tgatgaagcc ggggatatgt tttctgatct cagctgcaat gatctcagga tcaaagctca
    20581 tagcgctgac attgaatgca tttctgtgct tcagtctgga aggatctgcc tccatgatgt
    20641 tgatggcggc attaattgca tcaggcatgt acatcatgtc aaggaaggtt ccctttgaaa
    20701 ggggacaggt aaacctctga tgtttcactg cctcataata aatttccacg gcgtagtcag
    20761 tggttccccc gcccgggaga gtaacgtttg agatgatgcc gggaaacctg acaccccttg
    20821 tgtcaacgcc aaagcgaaga tggtaataat cacacagcat ctcgccggtt acctttgtga
    20881 tgccgtacat tgaagttggc ctctgaatgg tatcctgcgg ggtattgtca aggggggtgg
    20941 tgggcccgaa ggcgccgatg gaacttggga agaaaactgc gcagttgaac tcacgggctg
    21001 cctcaagtac attatagagc ccgttgaccc ctatgttcca tgccagcccg gggttctttt
    21061 ctcccacggc agagagtacg gctgcaaggt ggtagatggt gtcaatgtta tattttttca
    21121 ctgcggcggc aagttcctcc ctgttggtgg cgtcaacctt cataaaagga ccagtttcgg
    21181 ccagcgctcc ggtgggctgg gtcctgttaa tgcctgctat cacgttgccg ttgccgtacc
    21241 gctttctcag ttccagggta agctcagatc ctatctgacc taccgagccg gttaccagta
    21301 tgttcttcat tcttatctgt atgagttatg gttttctgtt catgtctgat catcagaaca
    21361 ggcacacaaa cttcatgatt ttttcggtaa cttgttctga taaaaatcat ctcattgtta
    21421 tccggataaa atagggccta tgaaacaaag ttgatatatt tgctcgtcta ataaccattc
    21481 acatagttaa tcaataatca catatccatg tacacaaatt ttcaggaatt tctgaccggt
    21541 gagctcagtg ctatcaaaga ggctggtctt tacaaggacg aaagaatcat tgtctcgccc
    21601 cagggggcgg agataaccct gaatacaggg cagaaggtgc ttaatttctg tgccaacaac
    21661 taccttggcc tctcggcaaa caaaaagctt gttgaggcag ccaggcaggc acttgacact
    21721 cacggttacg gcatgtcatc ggtccgtttc atctgtggta cgggtgatct tcacaaggag
    21781 ctggaacaga agcttgcaca cttcttcgga acagaggata ctatcctcta tgcctcctgc
    21841 ttcgatgcca acggcggagt gtttgaaccg ctgctcacca gtgacgatgc cataatcagt
    21901 gactcactca atcatgcttc aatcattgac ggtgtcaggc tctgcaaggc acagcgctac
    21961 cgttatgcca acgctgacat gaatgacctt gaggagaagc tgaaagaggc acaggcacag
    22021 cgcttcaggc ttatagtcac tgacggcgtc ttttcaatgg acggcaatgt ggcgccgctt
    22081 gacaggatat atgagctggc caataaatac aatgccatga tcatggtaga cgaatctcac
    22141 tcagcaggtg tggttggaga aacaggccgt ggagtcacgg aactctataa tctcaagggg
    22201 aaaatagaga ttatcaccgg cacccttggc aaagcctttg gcggtgcgat aggaggattc
    22261 acaaccggca aaaaggagat cattgatatg ctccgtcagc ggtccaggcc atacctcttc
    22321 tccaactcca ttcccccgct ggtggcagca gcaggaataa agatggtaga gatgatgaca
    22381 gagaccaacg agctgcagga caagctgcac ttcaacacca aatacttcat ggagaagatg
    22441 atgaaggcag gctttgatat caaaccgaca gagtcagcca tctgcgccgt gatgctctat
    22501 gacgccaaac tgtcacagga gatggctgcc agactcctca gggagggtat ctatgtcatc
    22561 ggcttctatt atccggtggt tcctaaaggt gaggcacgca taagggtaca gctttcggca
    22621 gcccacgata cagaacacct tgacaaggcc ataactgcat tcataaaggt cggtaaagag
    22681 cttaatatta tta
//
LOCUS       DAOPBK010000012        22513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2441_length_22513_cov_4.742675, whole genome shotgun sequence.
ACCESSION   DAOPBK010000012 DAOPBK010000000
VERSION     DAOPBK010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2441_length_22513_cov_4.742675"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="PLA17_01515"
     CDS             <1..1581
                     /locus_tag="PLA17_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK84121.1"
                     /translation="VSDWENFRKLPAAASMDEYELTDYNLGYVWYNTGDYAKALPHFM
                     AYINRADQRESPDMVIDARNRIADCYYIRADYPSAISHYDKVIDYGRVDADYAMFQKG
                     FAQGLNNNNAGKITTLTGLITKYPKSTYVPNALFERGRAYVATDQPARGEADFTNIIA
                     WYTGSQFVPPAMVQLGLIYYNAGENEKAVAQFKKVIENYRNTPEARNAVNGLRNAYSD
                     MDDVESFFAYMKNVEGLGDIEASTRDSMLYISGENQYIKGNCERSSEIFRNYLKEFPS
                     GSFATNARFYLADCLNRSGKVDEALDLYLRVISVGNNQFMEQSLLGAASISFGKKDYR
                     GAWSLYERLGREASSPENRLYAYLGMMRSASALGDDERVIASADLVLASEKLTEDLAR
                     EATFLTAKANQRLGNNDDALDDYRRVAREVATAYGAESKYRVAELLWIAGDIDGAEKE
                     ANQFVDMSSPHVYWTGKTFLLLSDIAIRKGDAFQAKATLQSLIDYYTVSDDGIIEEAR
                     ARLAALTAAQPSGAGTAN"
     gene            1587..3308
                     /locus_tag="PLA17_01520"
     CDS             1587..3308
                     /locus_tag="PLA17_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84122.1"
                     /translation="MVPNNQMMKKMKGKYYIMAVVMLITATTAAQTEDDNIKRTVTLY
                     NPYKPTLHEAVKRTLLPSDDDTTTVDIRFSYDFTPGSFVPEYEISPIKSAVLSPEPLP
                     DLKKGYVSLGLGNYLSPFVEVSISNGRSNTGAVGLFTRTYGSAGRIELENDRKVYAGF
                     LDNQALLYGKKYFRRSRLDSDIDFRHMTRHAYGYNPDVVGYDPDKKDIRSLYYDVTGK
                     VRYFTMEPDSSDLNWDASLKYNFFTRQGEGQQHNPGLSVSAGTNMFGLYAGVDAGYDM
                     YIFSKALDTKSRHLVTLAPYITKGNNDWRFRFGLKVAADAKENHDPFVGGVRKLYMFF
                     YPDAMFTFRIIPKYLRFTAAIDGFMENNQARNTAYVNPWMLPGDTLYNLRSTDNELRI
                     SAGLSGTMNVNASYSFGASYTLFKDLLLFMNDTIGYGNYFVPVYSDGSLLKIHGEVSV
                     PLNPQLTLSLLGNYYGYNLSGQEYAWHKPEWDGSLKAAYNLRNKIIASVDLTLTGTRY
                     ARVMAPGETIKLPFHPNLNMGIEYRYTPEISFWVKCNNISYNRYFEWNYYPSRNLMFI
                     GGISYSL"
     gene            3465..5447
                     /gene="gyrB"
                     /locus_tag="PLA17_01525"
     CDS             3465..5447
                     /gene="gyrB"
                     /locus_tag="PLA17_01525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPK84123.1"
                     /translation="MTNEELRKRSREQDEYSADSIQVLEGLEAVRKRPAMYIGDVGAR
                     GLHHLVYEVVDNSIDEALAGYCTRIDVTINEDNSITVMDNGRGIPVEMHEKEKRSALE
                     VVMTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSSRMTVEVMRQGKRWSQTYERGKP
                     LAPVAVIGDSDESGTITRFMPDNTIFQTIDFKFEILAARMRELAFLNRNILLTLTDKR
                     EHAENGGNAEQRHVTERFFSERGLIEFVEYLDSNRERLTENVIYIDYNKGDVPVELAL
                     QYNTSFSENVHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEDSGLTSKLKFDINGDD
                     FREGLTSVISVKVAEPQFEGQTKTKLGNSEVMGAVDVAISTALTHYLEENPRDAKAIV
                     QKVILAATARHAARKARELVQRKNVLSGSGLPGKLADCAERDPSVSEIFLVEGDSAGG
                     TAKQGRDRRFQAILPLRGKILNVEKAMQHKIFDSEEIKNIFTALGVYIGTDDDSKALN
                     TSGLRYHKVIIMTDADVDGSHITTLIMTFFFRHMKELIENGHIYIATPPLYLVRRGKS
                     EKYCWSEEERDAAVREMGQGKDSTVSVQRYKGLGEMNAEQLWETTMNPETRTLRQVTI
                     DSAAEADHIFSMLMGDDVPPRREFIGRHAKYAKIDA"
     gene            5482..5697
                     /locus_tag="PLA17_01530"
     CDS             5482..5697
                     /locus_tag="PLA17_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84124.1"
                     /translation="MSQNGRLTFRKSLNLADSSALIIGSMIGSGIFIVSADMSCTPGS
                     PGWLLVAGLIIVVIGIPVYYIWRWFNK"
     gene            5716..6636
                     /locus_tag="PLA17_01535"
     CDS             5716..6636
                     /locus_tag="PLA17_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPK84125.1"
                     /translation="MPDTNKTVGKFHNQRRINPVTDWIIRLLKGTLVGIGAILPGLSG
                     GVLAVIFGLYEPLLKFLADLKKDFVKNVLFFIPVIIGVAFGIFLFAIVVEKAFGRYAA
                     IFVCLFIGFVIGTFPSLYKTAGKKGRSTKDMVILIISALAIFTLMLVGEQQLVNVTPD
                     IPVWFGSGALIGLGLIVPGMSPSNFLIYFGLYDKMAAGINNFDMAVVIPLTAGVIVCV
                     LLFAKLASFLFRKYYSGMYHFILGMVVGSSLAIFPVIVFPAFKADMLSASGLSFGATV
                     ALCLVMLVVGSLATWLFSKVEEKYPREDLF"
     gene            complement(6696..7982)
                     /locus_tag="PLA17_01540"
     CDS             complement(6696..7982)
                     /locus_tag="PLA17_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK84126.1"
                     /translation="MTKLSLSIMILLLLLPIGAGAQRTMSLDEAIMEALENNYSLKIS
                     RNDEAIADNNVSLSPFLPTITGTGRQSQTTNNTRSSSAGEDKTRTNLYTAGVALNWRL
                     FDGFGMFTTYSRQQELLAMSSQRVKINVENLIMRVCSEYYNIIVQQNRMQSALTSLAL
                     SRARYENAEEKYLLGVISGLDLQQARIDLNADSSAVLTQQEIVVSAYIRMTNLLNAGH
                     EINFNINDTIILAPEIDIDSLRERTLSFNNQLILARQGEKLSEYDIESVRANRYPTVN
                     FSTGYNLTRNEFPWQASSYNQTNGLNWGFNLSWNIFSGLEVNRRMSNAKIEAENSRLA
                     YLDIENEILGELDLLYNTYRNNIIVTIFETQSAEVARASLEIAMERYRLGSLSGLEFR
                     EYQNNYLNAINRRLTALYQAKISEIGLRLLAGELTE"
     gene            complement(7963..11022)
                     /locus_tag="PLA17_01545"
     CDS             complement(7963..11022)
                     /locus_tag="PLA17_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK84127.1"
                     /translation="MSLSSTSIKRPVLATVMALVILIMGAIGYTFLGIRDYPSVDPAV
                     VTVSTSFPGANSDVIETQITEPLESAINSVQGIRSISSSSSDGSSRITVEFRLEVDME
                     TAVNDVRDKVSGAMRRLPQDVDPPVVQKADADAQPIFAVTLRSDTRSIIDVSQYAELN
                     FRERLQTISGVSAVNVWGSKKLAIRMKMDPARLAAYGLTPLDIRNAVTRENVELPSGR
                     IEGDNTELTIRTLGRLRTVDEFNDLIITKNGDRVVKFSDIGTAEIDAENIRSHLLSNG
                     KPAVNIVLVPQPGANYIDIVDNAQILLKQLEKDLPEDITMEVLFDNTLYIRESIREVK
                     NTIFIAFFLVVVVIFLFLRNWRTTLIPVVAVPISLIGAFFVMYLAGFTINILTLFAIV
                     LSISLVVDDAIVMVENIYTKVENGMKPMEAALAGSKEIYFAIIATTIALISVFSPIVF
                     LQGVTGRLFREFSIVIVGAVIISAFLALSLTPMISSRVLKPDALEGRFYHATRPFFTG
                     MINLYRKAITSFLKVRYWAVIIIVAAGGLIAWMWALLPSEMAPLEDRGYVNINARAPE
                     GASYEYMTAYMDQIAPVVREQVPESDNIMMMVGMGGVNAANMRVRLVDEKQRKRSQQE
                     IAATLSPVLRQYTGAQTIVSQQQTFGSRRGGLPVQYVIQAKNLDDLKRVIPDFLAMTS
                     VSPYFSASDVNLKFTKPELTVLINREKASRMGVNVQSIAQTLQLTMSEQRIGYYILDG
                     KQYQIFSQFDKSKRNDPFDLTSTYVRNDKGQLIQLDNIVEIRENSLPPQLYRYNRFVS
                     ATVSAGLAGRYTLGEGIEEMDRIAGEVLDENFSTTLSGDSKDFVESTSSLLFAFVLAL
                     VLIYLVLAAQFESFRDPVIILVTVPLALIGALFTMVVFSQTLNIFSQIGLIMLIGMVA
                     KNGILIVEFANQRQQAGLSVTDAVREASVARFRPILMTSLTTILGIMPLALATGAGSE
                     SRVTMGVTVVGGMFFATFLTLFVVPSVYSYLAGKKKAENSINDQTQP"
     gene            complement(11061..12287)
                     /locus_tag="PLA17_01550"
     CDS             complement(11061..12287)
                     /locus_tag="PLA17_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK84128.1"
                     /translation="MKKGTKITLWSTIALVLLIWFAGPRLGLWGNRDLKKKAGDTVVQ
                     APGQESGGRQGERPGRQQGQTRGSQQGQTPGGQQGQTRGSQQGQSQQPGSQGQLPVTG
                     IIAKPSYLTNGIRSAGSLLANEEVDIVSKVSGKVTAVYFREGSKVKKGDLLVKIYDED
                     LQAQLRRSEIQEKMLAEKLGRQRVLLSRDAVSREAFDQLQTDYNVILADINLLKVRIA
                     ETEVRAPFDGVIGFRFVSEGAYVTPQVKIAHLIDQSYLKLEFAISEKYVSENLMGKRI
                     SFETQGYREEFFATVYAVDYRIDQSTRTIGLRARFDNRAGKLVPGMFASLTLITDEKM
                     NALQVPTEAIVPEMNEKKIWVARNGRAELVPVVAGARTASSIEITSGLSAGDTVLTTG
                     LMHLRQNMAVRVNITD"
     gene            complement(12467..13900)
                     /gene="dacB"
                     /locus_tag="PLA17_01555"
     CDS             complement(12467..13900)
                     /gene="dacB"
                     /locus_tag="PLA17_01555"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPK84129.1"
                     /translation="MRTRLLTILILSLTAAASLSAQQQVMDSILADTTLTGTSYSFCF
                     ADAVTGEIIFSYDADRNLASASVMKLYPTSVALSLLGPCYRYTTNLLLTGELNTKKGI
                     LDGDVVIIGGGDPALGSDYFAEYYGDVTGEWVSALKEAGVKKVKGRVAAGESIYDFNP
                     APAGWAWGDLGQYYGAGVYDININDNEYNIYITGRAGGEAAIIDSVEDYGKEISLVSY
                     LISSGSTDRGYVYNAPYSAMAWVTGSVPADSSFALRASIPNPPFALVKRLDAAMRKAG
                     IAVDGEPVPERVSTEGKKSLSVAVTLSPTLAEIVKVTNHESVNMYAEALRKHLGREIL
                     GEGTWNAGSAVIRQFLDSIGCQPDQAVILDGSGLSSNNNISALMTVRLLVHMHNSSVS
                     EPFIASLPMGAVSGTMKYYFRDEIFKGRVVAKTGTITSAKSFAGYFTTNSGRIIAFTT
                     FANGFTVPSRKITDNMERVVREIMIKY"
     gene            complement(13897..14928)
                     /gene="rlmN"
                     /locus_tag="PLA17_01560"
     CDS             complement(13897..14928)
                     /gene="rlmN"
                     /locus_tag="PLA17_01560"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPK84130.1"
                     /translation="MMEKKKLFGLTLSELSEVVRDASLPPYAAKQIAGWLYHRGAPAI
                     GAMTDLSLAARKILEKDYETGLNPPVSESSASDGTKKYLFRVGENRFIETVFIPDHDR
                     ATICLSVQLGCRMGCRFCMTARQGFQGNLSANEILNQFRSLPEHDALTNIVFMGMGEP
                     LDNLEEVLRCLEILTSDWGYGWSPTRITVSTTGITGRIEEFLNKSKCHLAVSLHSPFD
                     SERQSLMPVQKAHPVKEVLDTIRGFDFGRQRRVSFEYILFRGVNDTPAHVRELARILE
                     GIRCRINLIRFHSIPGSEFTSPDQRGMEAFRDSLNAKGITATIRASRGQDINAACGLL
                     STLEQNKPK"
     gene            complement(14925..16022)
                     /locus_tag="PLA17_01565"
     CDS             complement(14925..16022)
                     /locus_tag="PLA17_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPK84131.1"
                     /translation="MKRLILKTLLVAAAIFCLNGIVRAQHENTELMNAINEVNRNFDA
                     LSHRLDVIEKSIDDLLWFDRVGDIAFIDKVYITGPPPAKESNPTGQGAGNPLKFWSYV
                     FIPRDINPSAKYPLLVLPHGGVHANFTTYHTHMIREMVSQGYIVVAAEYRGSTGYGAG
                     MYRRIDYGGLEVEDVDASRQYMIDNYEFVDKDRVGIIGWSHGGLIGLFCIFNHPEAYK
                     ACFAGVPVSDVVARMGYKNDGYRDLFSAPYHIGKTADQNVAEYRKRSPAWQADRYRNT
                     PLLIHTNTNDEDVNVLEVEHLIKSLKAADKKNFSYRIFENMPGGHSFDRMDYREARKI
                     RLEIYNFLNAELKPSKPFKNIRDLDRAGYRF"
     gene            complement(16037..16810)
                     /gene="dapF"
                     /locus_tag="PLA17_01570"
     CDS             complement(16037..16810)
                     /gene="dapF"
                     /locus_tag="PLA17_01570"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPK84132.1"
                     /translation="MVFNKYEGAGNDFIIVKNTPSIIAHDDALLIRKLCDRHFGIGGD
                     GVILIEECEGYDFRMSYFNSDGSPGEMCGNGARCAAHFAMRHITGFRDIAFMAGDGPH
                     TARPAGDGLTEVSIRDVSGIRHTPDGIVVNTGVPHLVVFTDDNSATDMVAFGRPLRWS
                     PAYAPEGVNVNLVSIRDGRLWVRTYERGVEEETLACGTGITASAIAAVYSGKIVTPAT
                     GVQTRGGMLSVRFNIDGERATDIFLTGPATFVFSGETEI"
     gene            16994..18439
                     /locus_tag="PLA17_01575"
     CDS             16994..18439
                     /locus_tag="PLA17_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPK84133.1"
                     /translation="MKKRDLITTLLVALFGALAGLFIFTRFLDRDKMVIGTEKERQMI
                     EENARYTMMMPQSGVNDFTFAAEQTVHVVVHVKTRATVSSSYSNPLYEFFYGRGSNQS
                     RVVRGFGSGVIVTEDGYIVTNNHVIEDADEVDVTLNDNRTFSAEIVGRDPSTDIAVIK
                     IKASSLPYIRFGNSDAIRLGEWVLAVGNPFNLTSTVTAGIVSAKGRSLGLLDNQYRIE
                     SFIQTDAALNQGNSGGALVNVQAELIGITTAIISPSGAYAGNSFAVPTSIVRKVYEDI
                     RQFGEVQRGLLGVNITDVTAELAKEENLREIKGVYVAGVTDDGAAKEAGLAEKDVIIA
                     INDEPVETTADLQEKVSRYRPGDKLEVTYLRKGRKDKKSVVLRNIEGGTGVLAPGTQS
                     STVFGATFTPVTAGEKSQYKISGGVKITSISDGRFRDLGLSKGTIIVDVNGVKVNSGA
                     DVRKATGDEKNLTSVEGFTPNGTYFKYQTRR"
     gene            18590..19450
                     /locus_tag="PLA17_01580"
     CDS             18590..19450
                     /locus_tag="PLA17_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPK84134.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEELISVEEEVELAQRIK
                     KGDRAALEKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFARFEQENERIPSPEELAE
                     VLDLPKEKVIDTLKVSGRHVSVDAPFVEGEDNSLLDVLTNIDSPIADKMLIDESLSKE
                     IDRALATLTERERDIIRYFFGIACQEMTLEEIGEKFGLTRERVRQIKEKAIRRLRHTS
                     RSKLLKGYLG"
     gene            19580..21595
                     /gene="uvrB"
                     /locus_tag="PLA17_01585"
     CDS             19580..21595
                     /gene="uvrB"
                     /locus_tag="PLA17_01585"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPK84135.1"
                     /translation="MEFRLTSDYTPAGDQPEAIRQLTDGLIQGVPCQTLLGVTGSGKT
                     FTIANVIAQVNRPVLVLSHNKTLAAQLYGEFREFFPENAVEYFVSYYDYYQPEAYLPV
                     TDTYIEKDLSINDEIEKLRLSATSSLLSGRRDVIVVSSVSCIYGIGNPDDFRSNTVHV
                     IRGAKISRTLLLRRLVDSLYARNELDFSHGTFRVRGDTVDVFPAYADVALRIVFFGDE
                     IEEVSTFDPLSGQHRDYLDEYVIYPANLFVTTRERINMAVSQIQDDMVLQARHFRDTG
                     RPEEAKRIEQRVQYDLEMIRELGYCSGIENYSRYFDGRAPGTRPFCLLDCFPRDFITV
                     IDESHVTVPQIRAMYGGDHSRKQTLVEYGFRLPAALDNRPLRLEEFEALTGQTIYMSA
                     TPAEYEMEKSEGVFVDQVIRPTGLLDPPVEVRPSRNQVDDLMEEIRKRSARNERVLVT
                     TMTKRMAEELNNYLARFDIKSRYIHSDVETIERVEILEGLRSGVFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSGRALTQTAGRAARNINGLVVMYADRITGSMQETIDET
                     KRRQAKQLRYNSEMGITPQQVVKRSGTMLKGLSHKGVELHAYTGPEKVDVAADPVVKY
                     MSREQLEKAIEKTRKAMEKAAAALDFIEAARLRDEIEGMRKLANERG"
     gene            21716..22492
                     /locus_tag="PLA17_01590"
     CDS             21716..22492
                     /locus_tag="PLA17_01590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK84136.1"
                     /translation="MAVLLTGACTRLPDPEPVDNPRVVILMYHRITEGEAGNLYERSV
                     EEFEKDLIYLRENNIRVIDFNELEAVVSGDLKLTTHAAVITFDDGDHSWYTHAAPLLK
                     NYRMKATFFLWASKIGMDSFLDWDEVKRLSSYTLDGGVRPFSFGSHTMSHHYLFNMKG
                     ALGGGEAFQEYLDEELGGSKVLIDRHIYGTVEALALPFGDGAGDEEIIAGARRHGYKY
                     IRTSERNVTGMAGADMFRLPSLPLLDDTEQKTIGTYLGIY"
BASE COUNT         5467 a         5393 c         6004 g         5649 t
ORIGIN      
        1 gtcagtgact gggagaactt caggaagctg cctgccgcag catcaatgga tgagtatgag
       61 ctgacggact acaacctggg gtatgtatgg tataatacag gagactatgc gaaagcattg
      121 ccgcatttca tggcatatat caacagggcc gatcagcgtg agagtcctga catggttatt
      181 gatgcccgca accggatagc cgactgttac tacatcaggg ctgattaccc gtcggccata
      241 tcacactatg acaaggtgat tgactacggc agggtggatg ctgactatgc aatgttccag
      301 aagggatttg cccagggtct gaacaataat aacgcgggca agatcaccac cctcacgggc
      361 ctgattacaa agtatccgaa atctacctat gtgccgaacg cactgtttga aaggggaagg
      421 gcgtatgttg ccacagacca gcctgcgcgt ggtgaagccg actttacgaa tatcattgcc
      481 tggtacaccg ggagccagtt tgttccccct gccatggtgc agctcgggct aatctactac
      541 aatgcggggg agaatgaaaa ggctgttgcc cagttcaaga aggtgataga gaattaccgg
      601 aacaccccgg aggcccggaa cgctgtcaac ggtctcagga acgcctattc tgacatggac
      661 gatgtcgagt cgttctttgc atacatgaaa aatgtcgagg ggcttggaga catagaggct
      721 tccacgcgcg actccatgct gtacatctcc ggggaaaacc agtacataaa gggtaactgc
      781 gagagatcat cagagatctt cagaaactac ctgaaggagt tcccgtcagg cagctttgcc
      841 accaatgcaa ggttctacct ggctgactgt cttaacaggt caggcaaggt tgacgaggcg
      901 cttgacctct atctcagggt gatcagtgtg gggaacaacc agtttatgga gcagtcattg
      961 ctcggcgcag catccatcag cttcgggaag aaggattaca gaggtgcatg gtcactttac
     1021 gaacggctgg gcagggaggc ttcaagcccg gagaacaggc tgtatgccta tctgggaatg
     1081 atgcgttcag cgtcagctct tggtgatgat gagagggtca ttgcctcggc agacctggtg
     1141 ctcgcttcgg agaagctgac ggaggatctg gccagggagg ccactttcct tacggcgaag
     1201 gccaaccaga gactgggcaa caatgacgat gcacttgatg actatcgccg tgtggcccgg
     1261 gaagttgcca ctgcctacgg ggcggagtcg aagtatcgtg tggctgagct gctgtggatt
     1321 gcaggagaca tcgatggtgc agagaaggag gctaaccagt ttgtcgacat gagttcacct
     1381 catgtatact ggaccgggaa gaccttcctg ctgctgtcag acattgccat aaggaaggga
     1441 gacgcctttc aggcgaaggc aacactgcag agcctgattg attactatac tgtcagtgat
     1501 gacggcataa ttgaagaggc cagggccagg cttgcagccc tgacagccgc acagccttca
     1561 ggagccggaa ctgctaactg aataagatgg taccgaacaa ccagatgatg aagaagatga
     1621 aaggaaaata ctatatcatg gcagttgtca tgctgatcac ggcgacaact gccgcacaga
     1681 cagaagatga taatataaag agaacggtaa ctctttacaa cccgtataag cccacactgc
     1741 atgaggctgt caaaaggact ctcctccctt cggatgatga taccactacg gttgacatac
     1801 ggttcagcta cgactttacc cctggttcat tcgttcccga atacgagatc agccccataa
     1861 agtcagcggt gctctcaccg gagcctctgc cggatctgaa gaagggttat gtaagcctcg
     1921 gtcttggcaa ctatctctct ccttttgttg aggtgagtat ctcgaacggc aggtcgaaca
     1981 cgggcgctgt cgggctcttc accaggacct acgggtcggc aggcaggata gagctggaga
     2041 atgacaggaa ggtctatgca ggattcctgg acaaccaggc gctgctgtat ggcaagaagt
     2101 atttcaggag gagcaggctt gacagtgata ttgacttcag acatatgaca cgccatgcct
     2161 acggctacaa tcctgatgtc gtcggttatg accctgacaa gaaggatatc aggtcactct
     2221 actatgatgt tacggggaaa gtcaggtact tcacaatgga gcctgattca agtgatctga
     2281 actgggatgc ctcgctgaag tacaactttt tcaccagaca gggggaaggg cagcagcata
     2341 acccgggact ctcagtcagt gcgggaacga atatgttcgg gctttatgcc ggtgttgacg
     2401 ccggttatga catgtatatc ttttcgaagg ctctggatac aaagtcaagg catcttgtta
     2461 ccctcgctcc gtacatcaca aaaggcaata acgactggag gttcaggttc gggctgaagg
     2521 tggctgccga tgcaaaggaa aaccatgatc cctttgtggg aggtgttcgc aagctttaca
     2581 tgtttttcta tcctgatgcc atgttcacct tcaggatcat tcccaaatac ctcaggttca
     2641 ctgctgccat tgacggtttc atggaaaaca accaggcccg gaataccgct tatgtcaatc
     2701 catggatgct tccgggtgat accctgtata atctgcgcag cacggacaat gagctgagga
     2761 tatccgcggg gctttcaggc acaatgaatg tcaatgcatc atactcattt ggtgccagtt
     2821 ataccctctt caaggatttg ctcctgttca tgaatgacac catagggtat ggcaactatt
     2881 ttgtgccagt atattcggac ggcagcctgt tgaagataca cggggaggtg tcagtgcccc
     2941 tcaatcccca gcttaccctt tcgttgcttg gcaactacta cggatacaac ctgtcgggtc
     3001 aggagtatgc ctggcacaag cctgagtggg acggatcatt gaaggctgcc tataatctca
     3061 ggaacaagat catagcttct gtcgatctta ccctgaccgg tacccggtat gcgagggtga
     3121 tggctccggg agagacgata aaactgccgt tccacccgaa cctgaatatg ggtatcgaat
     3181 acaggtatac acctgaaata tcattctggg tcaagtgcaa taatatttcc tacaaccggt
     3241 atttcgaatg gaactattac ccgtcaagaa acctgatgtt tatcggtggt atttcataca
     3301 gtctgtaaag tgtcagaaac cggtctcttt tgcccatttt ttttcatgaa ttattccctt
     3361 tttcgcaagg cagaaccggc attttttgct acctttgtat gatattataa gaaacatatt
     3421 atcaaactgt taaacagtaa aaaattcaat ttaaaactac ttttatgact aatgaggaat
     3481 tgaggaagag gagcagggaa caggatgagt attcagctga cagcatccag gttcttgaag
     3541 gcctggaagc tgtaaggaag cggcccgcca tgtatatcgg tgatgttggc gcaagggggt
     3601 tgcatcatct tgtatatgag gttgttgaca attccattga tgaagccctc gcgggatact
     3661 gcacccgcat agatgtgaca atcaacgaag ataactccat aaccgttatg gacaacggcc
     3721 gcgggatacc cgtggagatg catgagaagg agaagcggtc agcgcttgaa gtggtgatga
     3781 ccgtgctgca tgccggaggc aagtttgaca aggacagcta caaggtttcc ggtggtctcc
     3841 atggtgtagg ggtatcgtgt gtcaacgccc tctcatcccg tatgaccgtg gaggtcatga
     3901 ggcagggcaa gagatggtca cagacatatg agaggggaaa gcctcttgca ccggtagctg
     3961 ttattggtga ttctgatgag tccggcacca taaccagatt catgcctgac aacaccatct
     4021 tccagactat cgacttcaaa tttgagatac ttgcggcccg gatgagggaa ctcgcattcc
     4081 tgaacagaaa tattcttctt actcttactg acaagcgtga acatgcagag aacgggggaa
     4141 acgccgaaca gcgccacgtt accgagagat tcttctccga gagggggctg attgagttcg
     4201 ttgaatacct tgattcaaac agggagagac tgacggagaa tgtgatttac atcgactata
     4261 acaagggaga tgtgcctgtg gagctggcac tgcagtacaa tacatccttt tctgagaacg
     4321 tacactctta tgtcaacaac atcaatacca ttgaaggggg aacgcatctg gcaggctttc
     4381 gcaggggact gacccgtact ctcaagaaat atgctgagga ttccgggctg acaagtaagc
     4441 tgaagtttga catcaacggt gatgatttcc gcgaagggtt gacctctgtc atttcagtga
     4501 aggtggctga gccccagttt gagggtcaga cgaagaccaa gcttggaaac tcagaggtga
     4561 tgggtgccgt tgatgtggca atcagtactg cccttaccca ctatctggag gaaaatccca
     4621 gggatgcaaa ggcgatagtg cagaaagtga ttctcgctgc cacggcacgt catgcagcac
     4681 gcaaggcacg tgagctggta cagagaaaga atgtcctctc cggctccggc cttcctggca
     4741 agctggccga ctgcgcggag cgggatcctt cggtgtccga gatatttctc gtggaaggtg
     4801 actctgcagg cggaacggca aaacagggcc gtgaccgccg tttccaggct atccttcccc
     4861 tcaggggaaa gattctcaac gttgaaaagg caatgcagca caagattttt gacagtgagg
     4921 agatcaagaa catattcact gcccttggtg tttacatagg gacggacgat gacagcaaag
     4981 cgcttaacac ttcgggcctc aggtatcaca aggtaattat catgactgat gccgatgttg
     5041 atggctctca tatcaccaca ctgattatga ccttcttctt cagacatatg aaggagctga
     5101 ttgagaacgg acatatttac atagccactc caccgcttta ccttgtcagg aggggaaaga
     5161 gcgagaagta ctgctggtca gaggaggaac gcgatgctgc cgtaagggag atgggtcagg
     5221 gcaaggattc tacagtgtct gtgcagaggt acaagggtct tggtgagatg aatgccgagc
     5281 agttgtggga gacaacaatg aatccggaga cgagaaccct tcgccaggtt acaatagaca
     5341 gcgctgctga ggctgatcat atcttctcga tgcttatggg cgacgatgtg ccgccaagga
     5401 gggagttcat agggaggcat gcgaaatatg ccaagattga tgcataggca ggcaacagag
     5461 cggggtcaca gataattgta tatgtcacag aacggccggc tcaccttcag gaagagcctt
     5521 aacctggcag actcatccgc attgatcatc ggctcgatga ttggttccgg aatctttatt
     5581 gtcagtgcag acatgtcgtg cacccccggt tcgccgggtt ggctgctggt ggccgggctg
     5641 atcattgtgg tgatcggaat tccggtctat tatatctgga ggtggttcaa caaataaatc
     5701 attaatagga gagaaatgcc agatacaaat aaaacggtgg gaaaattcca taatcagagg
     5761 agaataaacc cggtgacgga ctggatcatc cgcctgctga aaggaaccct tgtaggtata
     5821 ggtgcgatcc tgcccggatt gtcaggcgga gtgcttgcgg ttattttcgg cttatatgaa
     5881 ccgctcctga aattccttgc ggatttgaaa aaggactttg tcaaaaatgt gttgtttttc
     5941 attccggtaa tcatcggagt ggctttcggt atattcttgt ttgccatagt ggtcgaaaag
     6001 gcatttggca ggtatgctgc catctttgtc tgcctcttta taggttttgt tatcggaaca
     6061 tttccctctc tttacaaaac agccggcaag aaggggagaa gcacgaagga tatggtaatt
     6121 ctgatcatat ccgcactggc tatcttcacg cttatgttag ttggtgaaca gcaactggta
     6181 aatgttaccc cggacatacc tgtctggttt ggatcaggag cgttgatcgg tctgggattg
     6241 attgttcccg ggatgagccc ctcgaatttc ctgatctatt tcggcctgta tgataagatg
     6301 gctgcaggta tcaacaattt cgacatggct gttgttattc ctctcactgc cggggtgatt
     6361 gtctgtgttc tgctttttgc aaagcttgcc tctttcctgt tcagaaagta ttattcaggc
     6421 atgtatcatt ttatcctggg gatggtggtt ggctcttcac ttgccatttt ccctgtcata
     6481 gtctttccgg ctttcaaggc tgacatgtta tctgcttcag ggttaagctt cggagcgaca
     6541 gttgcactgt gccttgtgat gctggtggtc ggctccctgg ccacatggct tttcagcaag
     6601 gtggaggaaa aatacccgcg ggaggacctt ttttagcacc tgaatatcgt tcccggtaac
     6661 aaaagcatcc ttgtcacggg atcaatggca gggatctatt ccgtaagctc accggcaaga
     6721 agccggagcc ctatctctga gatttttgcc tgatagaggg cggtcagcct gcggttaatg
     6781 gcgttaaggt agttattctg gtactccctg aattccagcc ctgacaatga gcctaaccgg
     6841 taccgctcca ttgctatctc aagtgaggcg cgtgccactt cggcactctg ggtttcaaaa
     6901 atggtaacaa tgatattgtt gcggtaggta ttgtacagca ggtcaagttc tccgagtatc
     6961 tcattttcga tatcaagata ggcaagtctg ctgttttcgg cctcaatctt cgcatttgac
     7021 atcctgcggt tgacctccag ccctgagaag atattccatg acaggttgaa tccccagttg
     7081 agcccgttcg tctggttgta tgacgatgcc tgccagggga actcattgcg ggtcagatta
     7141 tatccggtac tgaaattgac cgttggatag cggttggcac gtactgattc aatatcatat
     7201 tctgacagct tctcaccctg acgtgcaagg atcagctggt tgttgaaact gagagtacgt
     7261 tcgcgcagcg aatcaatatc gatctcaggt gccagtatga ttgtgtcatt gatattgaaa
     7321 ttgatctcat gacctgcgtt cagcaggttg gtcatccgta tataggcgct gacgactatc
     7381 tcctgctgtg tcaggacggc agagctgtca gcattcaggt ctatccttgc ctgctgaagg
     7441 tcgagccccg agataacacc gagaagatac ttctcctcag cgttctcata ccttgccctt
     7501 gacagggcca gtgatgtaag ggccgactgc atcctgttct gttgcacaat tatattgtag
     7561 tattcagagc agacccgcat tatcaggttc tcaacattga tcttcacccg ctggctgctc
     7621 atggccagga gttcctgctg cctgctgtag gtggtgaaca ttccgaaacc gtcaaagagt
     7681 ctccagttaa gtgccactcc tgccgtatag agattggttc tggttttgtc ttcaccggct
     7741 gaggacgacc tggtattgtt ggttgtctga gattgtctcc ccgttccggt gattgtggga
     7801 aggaaagggc tgagggagac gttgttgtct gctatagcct cgtcgttcct gctgatcttg
     7861 agtgaatagt tgttttcaag ggcctccatg atggcctcat caaggctcat ggtccgctgt
     7921 gctccggctc caatgggcag caggagcagc aatatcatta tactaaggct gagtttggtc
     7981 attgatgctg ttttctgctt ttttctttcc ggcgaggtag ctgtaaacgg agggaaccac
     8041 gaagagggta aggaatgtag cgaagaacat tcctcccaca acggttaccc ccattgtgac
     8101 acggctttcc gagcccgcac cggtggcaag ggccagcggc atgataccaa gtattgtggt
     8161 gaggctggtc atgagaatcg ggcggaaacg tgcaactgat gcctcacgta ccgcatccgt
     8221 cacgctcagt cctgcctgct gcctctggtt tgcaaattca acgataagga taccgttctt
     8281 ggctaccatc ccgatgagca tgatcaggcc tatctggctg aatatattaa gtgtctgact
     8341 aaaaaccacc atggtaaaga gagctccgat cagagccagt ggtactgtaa ccagtatgat
     8401 aaccgggtca cgaaagcttt caaactgggc cgccagaacc aggtagataa gcaccagcgc
     8461 cagcacgaag gcaaaaagaa ggctcgatgt gctttcaaca aagtcctttg agtcgcctga
     8521 cagtgtggtg ctgaagttct catcaagcac ctcgcctgca atcctgtcca tctcctcgat
     8581 accctcgccc agggtgtatc ttccggccag ccctgccgag acagttgccg acacaaacct
     8641 gttatacctg tacaactgcg gcgggagact gttttccctt atctctacga tattatccag
     8701 ctgaataagc tgccccttgt cgtttctcac atatgtgctt gtcaggtcga acgggtcatt
     8761 gcgttttgac ttgtcaaact ggctgaatat ctggtactgt ttgccgtcaa ggatataata
     8821 cccgattcgt tgttcgctca ttgtcagctg aagcgtctgg gcgatactct gtacgtttac
     8881 tcccatcctt gaagccttct ccctgttgat caaaacagtc agctcaggct ttgtgaattt
     8941 gaggttcaca tccgatgccg agaaataggg actcacggat gtcatggcaa ggaaatcagg
     9001 aattactctt ttcaggtcat cgaggttctt tgcctgtatg acgtactgca cgggcagacc
     9061 gccgcggcgt gagccgaagg tctgctgctg tgacactatg gtctgcgccc cggtatattg
     9121 cctgagcacc ggcgaaaggg ttgctgctat ctcctgctgt gaacgcttgc gttgcttctc
     9181 gtcaaccagt ctcactctca tgttggctgc gttgacaccc cccattccaa ccatcatcat
     9241 tatattgtca ctttccggca cctgctccct gaccaccggg gcaatctgat ccatgtaagc
     9301 ggtcatatat tcataggaag caccttcggg agccctggcg ttgatgttca cgtatccacg
     9361 atcctcgagt ggtgccatct ccgagggtag aagggcccac atccatgcaa tcagtccacc
     9421 tgccgccaca atgattatca ctgcccaata cctcaccttc aggaatgatg tgatggcttt
     9481 cctgtagaga ttaatcatac ctgtaaagaa gggcctggta gcatggtaaa agcgcccctc
     9541 cagggcatcg ggcttcagca cccgtgatga gatcatgggt gtaagtgaga gagccagaaa
     9601 tgctgagata atgactgcgc ctactatcac gatgctgaac tccctgaaga gcctgccggt
     9661 gactccctga aggaatacta taggagaaaa gacggatata agggcaatgg ttgttgcaat
     9721 gatagcgaag tatatttcct ttgagcctgc cagcgctgct tccattggct tcatgccatt
     9781 ttccaccttg gtgtatatgt tttccaccat cactatggcg tcatcaacca caaggctgat
     9841 tgaaagcact atggcgaaga gtgtgagaat gttgatggta aacccggcaa ggtacatcac
     9901 aaagaatgcg ccgatgagcg atatcggtac tgcaacaacg ggaataagtg ttgttctcca
     9961 gttgcggagg aagaggaaga tgaccactac caccaggaag aatgcaatga atatagtatt
    10021 ctttacctcc ctgattgact ccctgatata aagggtattg tcaaaaagca cctccatggt
    10081 gatgtcttca ggcaggtctt tctcgagctg tttgaggagt atctgggcgt tgtcaactat
    10141 gtcaatgtag ttggcgccgg gttgtggaac gagcacaatg tttactgccg gtttgccgtt
    10201 gctcagcagg tgtgaccgta tgttttccgc atcaatctcc gcggtgccga tgtcactgaa
    10261 cttcaccacc ctgtcaccgt tttttgttat gatcaggtca ttgaattcgt ctacggtgcg
    10321 caacctcccc agggtcctga ttgtaagctc cgtattgtcg ccttcaatcc ttccggaagg
    10381 cagttccaca ttctcccgtg taacggcatt acggatatcc agcggtgtga gcccgtatgc
    10441 agccagccgt gcaggatcca ttttcattct gatggccagt ttcttggatc cccatacgtt
    10501 cactgcactc acccctgata ttgtctgaag cctttccctg aagttgagct cggcatactg
    10561 gctgacatca attatgcttc tggtatcact cctgagcgtt accgcgaaga ttggctgggc
    10621 atcggcatca gctttctgga ctaccggcgg gtctacatcc tgcggcaggc gtcgcatggc
    10681 tcccgagacc ttgtcacgga cgtcattcac ggctgtctcc atgtccacct ccagcctgaa
    10741 ctcgacggta atgcgcgatg atccgtcact gctggagctt gagatggagc gtattccctg
    10801 cacactgttg atggcagatt caaggggctc ggtgatctgt gtctctatga cgtctgagtt
    10861 ggcgcccggg aaggaggtgg agacggttac gacagccggg tcgacgctgg gatagtccct
    10921 gatacccagg aaggtatagc cgatggcgcc cataatgaga atcacgagtg ccataacagt
    10981 ggcaagtacc ggccttttta tgcttgttga agaaagactc attcggtcag ttgttttttt
    11041 gaaactgtat tgtcaccggt tcagtctgta atattgaccc tgacagccat attctgcctg
    11101 agatgcatca gtccggtcgt aagcacggta tctccggcgg agagacctga cgtgatctcg
    11161 atggaagagg cggtcctggc accggcaacc accggtacaa gttcagccct gccgtttcgc
    11221 gccacccata tcttcttctc gttcatctcc ggcacaatag cctcggttgg tacctgcagg
    11281 gcgttcatct tctcgtcagt gataagggtc agcgaagcga acatgcccgg cacaagcttc
    11341 cccgcgcggt tgtcaaacct tgccctgagg cctatggtac gggtagactg gtcaatacgg
    11401 tagtcgacgg cgtagaccgt ggcgaaaaac tcttcacgat aaccctgggt ttcaaaagag
    11461 attctcttgc ccatcaggtt ttctgaaaca tatttttccg agatggcaaa ttcgagttta
    11521 aggtatgact ggtcaatcag atgcgcgatc ttgacctggg gagttacata ggcgccttcg
    11581 ctgacaaagc ggaaacctat aaccccgtcg aagggagcac gtacttctgt ctctgcaatc
    11641 ctgaccttca gcagattgat atctgccagt atcacattgt agtctgtctg gagctggtca
    11701 aaagcttccc ggctgacggc gtccctggag agcagaactc tctgacgccc gagtttttcg
    11761 gcaagcatct tctcctgtat ctctgaacgg cgcagctggg cctgaagatc ttcatcatag
    11821 atctttacca ggaggtctcc ctttttgacc ttgcttcctt cgcggaagta aactgcagtc
    11881 actttgcctg acaccttgct tactatgtct acctcctcgt ttgccagcag tgatccggct
    11941 gacctgatgc cgttggtgag gtatgatggt ttggctatta ttcctgtgac aggcagttga
    12001 ccctggctgc caggctgctg actctgcccc tgctgactgc cccgggtctg tccctgctgt
    12061 ccgcccgggg tctgcccctg ctgactaccc cgggtctgtc cctgctgtct gcccgggcgc
    12121 tctccctgcc ggcctcctga ttcctgaccg ggagcctgca ctacagtatc tccggctttc
    12181 tttttcaggt cacgattacc ccacagaccg agccgcggtc cggcgaacca tatcaacagg
    12241 acaagggcaa tggtactcca cagagttatt ttagtccctt ttttcatcta ttgttttgtg
    12301 ttcaattcct tgttcacaaa aatagagggg tttattaaaa ccatatgtta aagtatatta
    12361 aaacggagcg caggcgaata aataatgatg ctgtttcccc tggaagatgc ctcagtgttt
    12421 aatgataatc tcccttatca actactccat gtccttgtaa gatgcctcag tatttaatca
    12481 tgatctccct taccacccgc tccatgttgt cggttatctt tctcgacggt acggtgaatc
    12541 cgttggcaaa ggtggtaaat gcgattattc ttccgctgtt ggttgtgaaa tagcctgcaa
    12601 acgactttgc gctcgttatg gttccggttt tggcaaccac tctccctttg aatatctcat
    12661 cacggaaata gtatttcatg gttccggata cggcgcccat ggggagtgat gcaatgaagg
    12721 gttcagagac gctgctgttg tgcatgtgca caagcagcct gacagtcatc agggcgctga
    12781 tgttattgtt ggaggataat cctgacccgt caaggatcac cgcctgatct ggctgacacc
    12841 ctattgaatc gaggaactgc ctgatgacgg cagaaccggc attccaggta ccttctccca
    12901 gtatctcccg gcccaggtgt ttgcgaaggg cttcagcata catattgacg ctctcatgat
    12961 tggtgacctt tactatctcg gcgagggtgg gcgagagcgt aactgccacg gagaggctct
    13021 ttttgccttc tgtggagaca cgttccggca ctggttcacc atccactgct atgcctgcct
    13081 tacgcattgc ggcatctagt cttttcacca gggcgaaggg cggattgggg atggaggccc
    13141 tgagggcaaa gctgctgtcg gcaggcacgc ttcctgtcac ccaggccatc gcagagtagg
    13201 gagcattgta aacgtatccc ctgtcagtgc tgcccgatga gattaaatag ctgacaaggc
    13261 ttatctcttt accgtaatcc tccactgaat caatgatggc tgcctcgccc ccggcgcggc
    13321 ccgtaatgta tatgttgtat tcgttgtcgt ttatgttgat atcataaacc ccggcaccgt
    13381 agtattgtcc caggtcgccc catgcccatc cggccggtgc aggattgaag tcatagatgg
    13441 attcccctgc tgccacacgg cctttgacct tcttaacccc cgcttccttc agtgcactga
    13501 cccactcacc ggtcacgtcg ccatagtatt ccgcaaaata gtctgagccc agtgcagggt
    13561 caccaccccc aatgataacc acatcaccat caagtatgcc ctttttggtg ttcagctccc
    13621 ctgtgagcaa caggtttgtc gtatagcggt agcatggccc gaggagcgag agggccaccg
    13681 aggtagggta gagcttcatg acagaggctg atgcaagatt gcggtctgcg tcatagctga
    13741 aaataatctc gccggtcaca gcatcggcaa agcagaagga ataagaggtt cccgtcaggg
    13801 ttgtgtctgc aaggatggag tccattacct gttgctgtgc actgaggctt gctgcagctg
    13861 tcagtgaaag gataaggatg gtcagtaatc tggttctcat ttcggtttgt tttgttcaag
    13921 tgttgaaagc aggccgcagg ctgcattgat atcctgtccg cggcttgcac ggattgttgc
    13981 tgtgatgccc tttgcattca ggctgtcacg gaaagcctcc atcccgcgct ggtcaggact
    14041 tgtaaattcc gatccgggaa ttgaatggaa gcgtatgagg ttaatccggc agcggatacc
    14101 ttcgagtatc ctggccagct ccctgacatg tgcaggagtg tcattcactc ccctgaagag
    14161 aatgtattca aacgagactc ttctctgtct gccgaaatca aaacccctga tggtatcaag
    14221 aacctccttc accggatgtg ctttctgaac cggcatcagt gactgccgct cactgtcgaa
    14281 cggtgagtgc aggctgacgg ccagatggca tttgctctta ttgaggaact cctctatcct
    14341 gccggtaatg cctgtcgtgc tgacggtgat tcttgtcggg ctccatccgt aaccccagtc
    14401 agaggtcagt atctcaaggc agcggagaac ctcctccagg ttgtcaagag gttctcccat
    14461 ccccatgaat acgatatttg tcagtgcgtc atgctccggc aggctgcgga actggttcag
    14521 tatctcattt gcgctgagat ttccctggaa gccctgccgt gccgtcatgc aaaaacggca
    14581 tcccatgcgg cagcccagct gtaccgacag gcagatggtt gcccggtcat ggtcaggaat
    14641 aaagaccgtt tcaatgaacc ggttttcgcc aacacgaaag agatacttct ttgtgccgtc
    14701 actggcagat gattcagata caggcgggtt aaggcccgtt tcataatcct tttccaggat
    14761 tttgcgcgct gccagggaga gatcggtcat cgcgccgatg gcaggcgctc ccctgtgata
    14821 gagccatcct gctatctgct tcgccgcata cggcggcaga gaggcatccc tgacaacctc
    14881 tgacagctca ctgagagtca ggccgaatag tttcttcttt tccatcagaa ccggtagccg
    14941 gcccggtcaa ggtcacggat gtttttgaat ggctttgaag gtttcagctc tgcattcagg
    15001 aagttgtata tctcaagcct gatcttcctc gcctcacgat agtccatcct gtcaaatgag
    15061 tgccctccgg gcatgttttc aaaaatcctg taactgaaat tcttcttgtc tgcagctttg
    15121 agtgatttga tcaggtgttc cacctcaagt acgtttacat cctcatcatt ggtgtttgtg
    15181 tggatgagga ggggggtgtt gcggtaacgg tctgcctgcc aggccgggga gcgctttcgg
    15241 tattccgcca cattctgatc agccgttttg cctatgtgat agggtgctga gaagaggtca
    15301 cggtatccat cgttcttgta tcccatccgg gcgacgacgt cactgacagg cactcccgcg
    15361 aagcaggcct tgtatgcctc ggggtggtta aatatgcaga agagccctat caggcctccg
    15421 tggctccagc caataatccc gaccctgtcc ttgtcaacaa attcataatt atcgatcatg
    15481 tactggcggc tggcatcaac atcctccact tccaggccgc cataatctat cctgcgatac
    15541 attccggcgc cgtaccctgt ggagcccctg tattctgcag caacaacaat atacccctgt
    15601 gacaccatct cacggatcat gtgcgtatgg taggtggtaa agttagcatg cactccaccg
    15661 tggggaagga ccagaagcgg gtatttggcg gatggattga tgtctcgcgg gatgaagaca
    15721 taactccaga acttgagcgg attaccggcg ccctgtccgg tcgggttgct ctcctttgcc
    15781 ggcggcggtc cggtgatata gaccttgtca atgaaagcga tgtcacccac gcggtcgaac
    15841 cacagcaggt catcaatgct tttttcaatc acatcaagcc ggtgagacag ggcatcgaag
    15901 tttctgttca cctcattgat ggcattcatc aattcggtgt tttcgtgttg cgcccttacg
    15961 atgccgttaa ggcaaaaaat cgcagctgca accaggaggg tttttaaaat aagccttttc
    16021 atatcaattt ggtttattat atctctgttt ctccgctgaa gacaaaggta gccggaccgg
    16081 taaggaaaat atccgtagct ctttctccgt caatattgaa cctgaccgac aacattccgc
    16141 ccctggtctg caccccggtt gccggtgtga caatttttcc gctgtacact gccgcaatgg
    16201 cagatgctgt tattccggtt ccgcaggcaa gtgtttcttc ttccactccc cgttcgtagg
    16261 ttcgtaccca aagccttccg tccctgatgc ttacgaggtt gacgttcacc ccttcaggtg
    16321 cataggcagg tgaccagcgg aggggcctgc cgaaggcgac catgtcagtt gccgagttgt
    16381 catcggtgaa caccaccagg tggggcactc cggtgttgac aactattccg tcaggagtat
    16441 gcctgattcc gctgacgtca cgtattgata cctctgtcag cccgtcacct gccggcctgg
    16501 ccgtatgagg tccgtcgccc gccatgaagg caatatcgcg gaaacccgtg atatgcctca
    16561 tggcaaagtg tgccgcgcac ctggcaccgt ttccgcacat ttcaccgggt gaaccgtcag
    16621 agttgaaata agacatcctg aagtcatacc cttcgcactc ttctatgagt atcactccgt
    16681 ctccccctat tccgaaatgg cggtcgcata acttcctgat aagcaatgca tcatcatgcg
    16741 caataatgga aggagtgttt tttacaatta tgaagtcgtt gcctgctccc tcatatttgt
    16801 taaagaccat tttcatttcg ttaaattgaa gttaattatt ttgaaaagag cgcctcagtt
    16861 tgttaaaaaa ttgaaaattt gtagaacaat gcttctatgg aagtgttgta aagatattaa
    16921 tcttttgggc aggcacacta tttgattaaa tcagtaagga tcaaagaaag gcataaccaa
    16981 aaatataaaa acaatgaaaa aaagagattt gataactact ttgctggttg ctcttttcgg
    17041 agcgctggca gggcttttca tctttaccag gtttcttgac agggataaga tggtgattgg
    17101 gactgagaag gagaggcaga tgattgagga gaatgcgagg tatacgatga tgatgcctca
    17161 gtcaggggtg aatgatttta catttgcggc agagcagaca gtgcatgtag tggttcatgt
    17221 caagaccagg gcgacagtca gttcctcata ttcgaaccct ctttatgagt ttttttatgg
    17281 caggggttca aaccagtcaa gggtggtgcg tgggttcggc tcgggtgtga tagtcactga
    17341 agacgggtac attgttacca ataatcatgt catagaggat gctgatgagg ttgatgtgac
    17401 gctgaatgac aatcgtacct tttcagccga gatcgttggc cgtgatccga gtacggatat
    17461 agcggtcatt aagataaagg ccagctctct tccgtatata aggttcggta attcagatgc
    17521 aatacgtctc ggggagtggg tgctggcagt agggaatccg ttcaacctga catcgacggt
    17581 tacggcgggg atagtaagcg ccaagggaag gagcctgggt ctgcttgaca accagtacag
    17641 gatagagtca ttcatccaga ctgacgccgc cctcaaccag ggcaacagcg gaggtgcgct
    17701 ggtaaacgtt caggctgagc tcataggcat caccactgca attatttcac ccagcggcgc
    17761 ctatgccggc aactctttcg cagttcccac gtctattgta aggaaggtct acgaggacat
    17821 cagacagttc ggggaggtgc agcggggtct gttgggtgta aatatcaccg atgtcactgc
    17881 tgagctggct aaagaggaaa acctcaggga gataaagggt gtttatgttg cgggggtgac
    17941 ggatgacggt gctgcgaaag aagcagggct ggcagaaaag gatgtgatca ttgcaatcaa
    18001 tgatgaaccg gttgaaacca cggcggatct tcaggagaaa gtgagccgtt acagaccggg
    18061 cgacaaactc gaggtgacct acctcagaaa gggccgtaag gataaaaaga gtgtggttct
    18121 ccgcaatatt gagggtggta ccggcgttct agctcccggg acacaatcct caacagtgtt
    18181 cggcgccaca ttcacgccgg tcactgcagg cgagaaaagt cagtataaaa tatcaggagg
    18241 tgtaaagatc acttcgattt cggatggcag gttccgcgac cttggccttt cgaaaggaac
    18301 gatcattgtt gacgtgaacg gagtaaaagt gaacagcggt gcagatgtcc gcaaagcaac
    18361 aggtgacgag aagaatctga cctcagtgga aggatttaca cccaatggaa cgtacttcaa
    18421 gtaccagaca aggaggtaga aataaggcgt aattacaagg atcagaagat cagtgaatta
    18481 aatgaaattg cgaataccat gcagtgtggt tgaatatata tttgtttagt aacttaataa
    18541 gaagtatctt tgcgaaaatt ttttcgaagg gatatagaga atatagagaa tgagacaact
    18601 taaaattacc aaatccatta ccaaccgtga aagtgcgtcg ttggacaagt atctgcagga
    18661 gattggtaag gaagagttga tatcggttga ggaagaggta gagttagcac aacggatcaa
    18721 gaagggtgac agggcagcgc tggagaagct taccaaagcc aatcttcgtt tcgttgtttc
    18781 agtggccaaa caatatcaga atcaggggct tagtcttccc gacctcatta atgagggtaa
    18841 cctcggcctt atcaaggctg cagaaaaatt tgatgaaacc aggggtttca agttcatatc
    18901 gtatgcagtc tggtggatca ggcaatcaat ccttcaggct ctcgcggagc agtcaaggat
    18961 agtgcgtctt ccactcaacc aggttggctc actgaacaag atcaataagg catttgcaag
    19021 gtttgaacag gagaatgagc gtattccttc cccagaggag cttgccgagg tgcttgacct
    19081 gccgaaggag aaagtaattg acactctcaa ggtatcgggt cgtcatgtgt cagttgatgc
    19141 accctttgtg gaaggtgagg acaacagcct tctggatgtg ctcaccaaca tagattcacc
    19201 gattgccgac aagatgctga ttgacgagtc tctctcaaag gagattgacc gtgcactggc
    19261 aacacttacc gagcgtgaga gggatataat acgttatttc tttggcattg cctgccagga
    19321 gatgactctt gaagagatag gggagaagtt tggccttacc cgtgagaggg tcagacagat
    19381 caaggagaag gccattcgca ggctgcgtca tacatcccgc agcaaactcc tcaagggata
    19441 cctgggttag tattcccggg gcaaggtaaa cagataaaag aggtgcgtca gcatctcttt
    19501 tttcatatat atactgtctg tgcggagggt gatgattgtg acgggtctct ttttgttatt
    19561 tttgccctac cggagaatca tggaattcag gcttacatca gactatacac ccgcaggtga
    19621 tcagccagaa gccatacggc agctgaccga cgggctgatt cagggggtac cctgtcagac
    19681 actgctgggg gtgaccggat caggaaagac ctttaccatt gccaatgtaa ttgcacaggt
    19741 caacagaccg gtgctggtgc tgagccacaa caagacgctc gcagcgcagc tgtacggcga
    19801 attcagggag ttcttcccgg aaaatgcagt ggaatacttt gtgtcatatt acgactacta
    19861 tcagccggag gcttatctcc ctgttacgga cacctatata gagaaggacc tttcaataaa
    19921 tgatgaaata gaaaaactca ggctcagcgc cacctcgtca cttctttccg gcaggcgtga
    19981 tgtaattgtg gtctcttctg tatcctgtat ctacggtatt ggcaatccgg atgatttccg
    20041 ttccaatacc gttcatgtga tcaggggggc gaagatcagc cgtacgctgc tgcttcgcag
    20101 gctcgttgac agtctgtatg cacgcaatga actggatttc agccacggca cattcagggt
    20161 caggggagat accgtagatg ttttcccggc ttacgccgac gtcgccctgc gtatagtttt
    20221 tttcggtgac gagatagaag aggtatccac tttcgatccg ctgtcaggtc agcatcgtga
    20281 ttacctggat gagtatgtga tctatccggc gaaccttttt gttactacca gggagcgtat
    20341 caacatggct gtatcacaga tacaggatga catggtgctg caggccaggc acttccggga
    20401 taccggccgg ccggaggagg ccaaaaggat tgaacaaagg gtacagtatg accttgagat
    20461 gatcagggaa ctgggttatt gcagcggtat agagaactat tccagatact ttgacggcag
    20521 ggctccagga acaagaccct tctgcctgct tgattgtttt ccaagggatt tcataacggt
    20581 gatcgacgaa agtcatgtca ccgttcccca gataagggcg atgtacggag gtgatcactc
    20641 gcgaaagcag accctggtgg aatatggctt ccgcctgccg gctgcactgg acaacaggcc
    20701 gctgaggctg gaggagttcg aggcgctgac cggtcagacg atatacatga gcgcaacacc
    20761 ggccgaatat gagatggaaa agagcgaagg ggtattcgtt gaccaggtga taaggcccac
    20821 agggctcctt gatccgccgg tggaagtgcg gcccagccgc aaccaggtgg atgatctcat
    20881 ggaggagatc aggaaacgct ctgccaggaa cgagagggtt ctggttacaa ccatgacaaa
    20941 aaggatggca gaggagctca ataactacct cgccaggttc gacatcaaat cgagatacat
    21001 ccattcagat gttgaaacca ttgagagagt ggagattctg gaggggctca gatcgggcgt
    21061 gtttgatgtg cttgtaggtg tgaaccttct ccgcgagggg cttgaccttc ctgaagtgtc
    21121 actggtggca atactcgatg ccgacaagga gggtttcctc aggtcaggaa gggcgctgac
    21181 acagacggca ggcagggcgg caaggaatat taacggattg gtggtgatgt atgctgacag
    21241 gattaccggc tcaatgcagg agactatcga tgagacgaaa agaaggcagg ccaaacagct
    21301 caggtataac tctgaaatgg gaataacacc gcagcaggtg gtgaagagaa gcggaaccat
    21361 gttaaagggg ctgagccaca agggtgtgga gcttcatgct tacaccggtc ctgaaaaggt
    21421 tgatgtggca gctgatcctg ttgtgaagta tatgtcaagg gagcagcttg aaaaggcgat
    21481 tgaaaaaacc cgcaaggcga tggagaaggc tgctgcagcg ctagacttca ttgaggctgc
    21541 caggctgcga gatgagatcg agggtatgcg gaagctggca aacgaaagag gatagcctgc
    21601 aggaagggta cgaaaagagc cgttctgccg tttgatacta ttggcggtgt tccggcaagg
    21661 gccagaagtt gaatacaaat tgatcaatat gcagaaaact gttacggcct taatcatggc
    21721 ggtactttta acaggagcat gcaccaggct gcctgatcct gaacctgttg ataatcccag
    21781 ggtagtgata ctcatgtatc accgtatcac ggagggtgaa gcgggtaatc tttatgaacg
    21841 tagtgttgag gagttcgaaa aggatctcat ttacctcagg gaaaacaata tcagggtgat
    21901 cgacttcaat gagctggagg ctgtggtgtc aggcgatttg aagcttacca cccatgcagc
    21961 agtgattact ttcgatgacg gcgatcattc atggtacact catgctgccc cgctgctgaa
    22021 gaattacagg atgaaggcaa ccttctttct ctgggcctct aagatcggga tggattcgtt
    22081 ccttgactgg gatgaggtga agcgtttgag cagttacacc ctcgacggcg gtgtaaggcc
    22141 attctccttc gggtcacata ccatgtcaca ccactatctt tttaatatga agggagccct
    22201 tggcgggggt gaagccttcc aggagtacct tgacgaggag cttggcggtt caaaggtgct
    22261 tattgacagg catatctatg gcactgttga agcccttgcc ctgcctttcg gcgacggagc
    22321 aggtgatgag gagatcatcg caggagcccg gcgtcacgga tacaaataca tccgcacatc
    22381 cgaacggaat gtgacaggaa tggcaggagc tgacatgttc aggcttccaa gcctgccgtt
    22441 gcttgatgat actgagcaga aaactatagg cacttacctc gggatttatt gacaggtatc
    22501 ccgactaacc ctg
//
LOCUS       DAOPBK010000013        22388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2461_length_22388_cov_5.840595, whole genome shotgun sequence.
ACCESSION   DAOPBK010000013 DAOPBK010000000
VERSION     DAOPBK010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2461_length_22388_cov_5.840595"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PLA17_01595"
     CDS             complement(<1..1342)
                     /locus_tag="PLA17_01595"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPK84137.1"
                     /translation="MEKTLISALLLCVPLVIGAQPFNTFPVTTQKPPLHGRHWMAITG
                     KPLAATAGAMIFDRGGNAVDAACAMLAATCTMWDVLSWGGETQALIYNPKTGKVLAIN
                     ALGVAPTGATAEFYKSKGYKYPPEYGPLAAVTPGTPGGLMVMLAEYGTMSLKEVLAPA
                     MEMAAGYPIEAQTANSIERAKNRIKEWPYSAKVFLPHAGEEREAPSAGEVFVQKELLA
                     TLTKLVEAEQQALKKKKSRRDAIYAAYDRFYNGDIADEFVRGCQEQGGLITKEDLARW
                     QVKIEEPLMTEYKGIEVYKLQQWTQGPVMLQTLNILENFNLKEMGYNSTRYIHTLYQA
                     MNLAFADRDFYYGDPAFPPEEPMQGLLSKDYARERSKLINPDRNDPAVRPGDPYPFEG
                     KVNPFSTLLDSWQADARIIYPSDDPERYSSFMSKFQSGTTSIEAADEEGWVVSVT"
     gene            1523..2599
                     /locus_tag="PLA17_01600"
     CDS             1523..2599
                     /locus_tag="PLA17_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HPK84138.1"
                     /translation="MNPELYFWFAFILIFVVGFWVDMRAAKRRHAEESVSTALKWTGF
                     WISIAFLFGLAILLFFPQNENSTISTRSMMGVKFYSAYLTEYMLSVDNLFVFIMIFTM
                     MSVPGEIQPILLKYGILLSIFFRTLFILIGMGLINRFEWLIYLFGAFLIYMTFKMMKS
                     HDEDETIDPEKNTLIRAAKKLFQVDVNNKEPKFHSRVNGKFAITPFALTFLLIGSTDV
                     MFAIDSVPAVIGVIREGNHALTLEESNFLAISSNTFALMGLISLFFALKGIMKMFRHL
                     KTGICIVLFFVGAKMLLAGVDSVNMFFARHSWVSLAVILSTLALSILYSVVETRIIEK
                     REAKLATGNGTVDTLDALEDKKEE"
     gene            2796..3434
                     /locus_tag="PLA17_01605"
     CDS             2796..3434
                     /locus_tag="PLA17_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK84139.1"
                     /translation="MNEFDIKAAEWDKSQMRRERAEAVAIAIDRQIPLDRNMTAMEFG
                     AGTGLLSFMLLDKLGSVTLIDSSEGMVNVVREKLSGSSAASAMKVVKADLEKEEYPDE
                     IFDLIYTLMVLHHVNDVEKIISKFSSMQRSGGYLAIADLNSEDGSFHGNGFTGHRGFD
                     PGYLATILERHGYAGIRHSTVYNIGKTLPDGTRKEFGVFLITAVKKQVLPAD"
     gene            3465..3863
                     /locus_tag="PLA17_01610"
     CDS             3465..3863
                     /locus_tag="PLA17_01610"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPK84140.1"
                     /translation="MADIEITFDGGKVITAHVNGKTIKTDQSVKHGGGGTAPEPFELF
                     LASIGTCAGIYVKSFCDQRGINGEGIKIIQSVEIDPDKRVPSRFRLNIQLPADFPEKY
                     RTAVINAAELCLVKKTISNSPQFEITSSVS"
     gene            4101..4616
                     /locus_tag="PLA17_01615"
     CDS             4101..4616
                     /locus_tag="PLA17_01615"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPK84141.1"
                     /translation="MTERMKEVKASDLTDNLIRLIASDWMLVTAGTREKFNTMTASWG
                     GMGHLWNRNVAFVFVRPERYTYDFIESAEGFTLTFFDENYRDALNLCGTKSGRDCDKV
                     AEAGLTPFFTGSGLPAFAEARIIIECRKLYSAQLTREAFIDSEPLNKHYSAEDKLHKM
                     YIAEIVRVWKK"
     gene            complement(4926..6407)
                     /locus_tag="PLA17_01620"
     CDS             complement(4926..6407)
                     /locus_tag="PLA17_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPK84142.1"
                     /translation="MRKLLVLLALSLLVFPSRAQEISEETLLKAMHTISSHDLLEYVM
                     IQCDEKYQGRLTGTAEYQACAEWLAGEFESWDISPAGDEGGWFQWFDIPYTMVYPDCG
                     LTLHVPLKKDEVLLKHYNYVTEYMPGSTSGNGEVTAEVVYAGYGITAPELNYDDYRNI
                     DVKGKIVLVERESPVGPSAGEEKFMPWYEYSFHQHKLANAVKHGAAGMIYNYGPIANP
                     NNDYYEDFVYVHIGDTVVRDIFAGTGYNHRDVVRQINSTLKPNSFNTKKVVTVKMSTR
                     HFPDGKGSNVIGILKGTDPVLKDETIIIGAHLDHLGLCYEMIPGANDNASAVAVMMGV
                     AKALATNKIPLKRSVMFLSFGAEEQAIMGAYEYIKQPVMPLEKSVLLNMDGVGVGHSI
                     RAAAGTNYPKLWSFVEEANNKYIHRPLSTSYFSNLGRPRLDAARFIGAGVPSLSFSTY
                     GSTNYYHIPLDNIDIIKPEIMEDLAQLLLVTVVNMANSPDPLR"
     gene            complement(6567..7589)
                     /locus_tag="PLA17_01625"
     CDS             complement(6567..7589)
                     /locus_tag="PLA17_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84143.1"
                     /translation="MKVLVIYNIDHNWDATEINDTRNSNTVLAEALAKEGFETYLEEL
                     SNPHLECILEKHSPEDTIVFNLCEALPGIPFSEKTVTEILSDRGFTYTGNGPEVIDLS
                     YDKQKTKERIISLGIPVPEGAVLTPEEAGSWTLFPSIVKPSREHCSLTITEKSVVFDT
                     NSLREQILLVNKELNQPAMVEDFIDGREFHVSVWNNNPPEILPVAEMDFSAFTEPGER
                     LCTYDSKFIPGSEHYEKIETLIPAPLDGHLSRRLGEKVLAAWKGFGCNDYARFDLRLR
                     DGVFYLLDVNPNNDISFDTSFALAAEKNNYSYSRMVKRIVMMSAMRHPLFGAVEYPIV
                     TDEKKL"
     gene            complement(7690..8877)
                     /locus_tag="PLA17_01630"
     CDS             complement(7690..8877)
                     /locus_tag="PLA17_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPK84144.1"
                     /translation="MMKHLILITGLALLIPPGSWAQQANVNLDYNPQKNTENLVPFSA
                     SVNSPEVRDDNMVTFRLKAPNAQKVVLPVEAIHTANNLGREPIPFTKGDDGVWTLTIG
                     PLKPDMYAYHFNVDGIQIADPNNTYAAFTAMPPYSELIVHGDGPAYYDARNVPHGTVT
                     RHVYYSEVTGGERELYVYTPPAYNPKKKYPVLYLLGGSGELPSNWIYDGRANFILDNL
                     LAEGKAVPMIIAIPNNQIIHRNHPRHTELTFDIFEKELRNHVIPLVDKAYSTIRLPKG
                     RAISGLSMGGRHSMLIGFRSLDLFASFGILSAGDINAETSLAHFLNDPKVNEKVDYLF
                     VGQGTEEANGFMGQRCLALHEALVKHNIKHHYYTGGHGGHDWSTWRHLLYYQFLPNLW
                     RGK"
     gene            complement(9132..12941)
                     /locus_tag="PLA17_01635"
     CDS             complement(9132..12941)
                     /locus_tag="PLA17_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPK84145.1"
                     /translation="MKTRRNQLFLLVLFSILIMAFMPGSEKAKKPIYLNPAYSFKERA
                     ADLVSRMTPEEKQSQLGNTMAPIPRLGVNHYDIWGEALHGIMGRNNNSGMTATSFPNS
                     VAVGATWDPELIKRETKVISDEARGFNHNLIFTLTYWSPVIEPARDPRWGRTAETFGE
                     DPFLVSQIGKGFIQGLMGDDPVYLKAVPCGKHYFANNTEFNRHNGSADMDDRDMREYY
                     LLPYRTLIRDYKLPSIMTAYSAVNGIPVSASKYLVDTIARKTYGLDGYVTGDCGAIDD
                     MVRGHNYAENHVVAAALGIKAGVDTDCGSVYQANALEAVRLGILTEAEIDRALVNFFT
                     IRMRMGEFDPREIVPYAGIKPGIINDPSHNDLALEVATKTPVLLKNSTITEAGGKALP
                     IDLKRVRKIAVLGPQADKVELGDYSGEVEPHLSISPLEGIQNYIKEHNLNTEIVSVIS
                     GNTDRKTDFLTVNRFSTVRNGEVLEEYDATQFSSSAPGLIVASRYGWTVISGIKDGDW
                     TAYDNIDITNIDSIRFNLSSSADGGILEVRVGSAAGNVLASERISSPPDRGGFPGWAR
                     PRVVSVKINTLGITGPQTLVLVYREAEKEIDAAALDLAAGCDVALVFVGTDQSTGREE
                     SDRFALTLPGNQNELIKAVAAINPNTIVVMQTMGMVEVEQFKNLADVTGIIWTGYNGQ
                     AQGTAMAKILFGDVNPGGKLNVTWHKSLNDLPDFNDYSLRGDGTNGRTYMYFNKDVSY
                     EFGYGLSYTTFAYSNFAISKTSITPNDKITLSVDVQNTGTVDGEEVVQVYVKTPDSHA
                     SLERPIKRLKGFKRVMIPRGQVKTVTIDINCEDLWFWDSEKGKITFDPGRYIFEIGAS
                     SRDIKGEVEARMSGTYKPVLTTVVAGCDRVVLRPGDVVQTSVTASMSDDSFYDITKAG
                     ITFKSNNPGVAAVDEKGRVTATGVGVASIFAYVTIDGTTVSNSYPVKVMPDLNPGSVT
                     VNGKRIPGFDKEVKAYSYLLKSNSKIPVVKAEAPGSGVSVEIEQARAIPGTATVQLID
                     NVTLEKNVYYLNFDTESVSDEFNGGTVDPRWKWVRENKSTRSLSVKPGSLTITGEPGG
                     VLEATNNARNLLLQSANNDWTIETRLVCSRSPSQPENAGILAYQDDDNFVKLMFRAVV
                     KTTRVRGVQPGTIDLVMEENGIAKSLATFNLRNEVTGDNALSLKLEKRGSIYTAYYSL
                     DGTNWELLGTASLLLKDIRAGLIVCDGTVAQYMKSTFWFDSDTTKPDTPFDVSFDYFR
                     IKNSGLK"
     gene            complement(13333..15921)
                     /locus_tag="PLA17_01640"
     CDS             complement(13333..15921)
                     /locus_tag="PLA17_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPK84146.1"
                     /translation="MKTRSYMSGFILTVVIAVAALVSCNRADREEYTAEGVSKLLATY
                     RFETISDVRYNLIFTIDTGKSTPVEGTVMIDFVRRSGREPLVLDFRVPGDHLRSVSVN
                     GREVEAALTNGHIVIDRGLLSRRYNRVEVSFRAGDLSLNRNDEYLYTLFVPDRACTAF
                     PCFDQPDLKGRFTLALDIPESYRAMSNNPIVSAVTTDGRVTLRFSETKPISTYLFAFA
                     AGRFELVEKEIEGVKMEMLHRENRPGYVENNSDEIFRLHYSALKWLEEYTQIPYPFDK
                     FGFVLLPPFQYSGMEHPGSIFYRASSLLLEASPTLNEKLSRASLIAHETSHIWFGDLV
                     TMKWFDDVWLKEVFAGYMSDKIAGPDFPDVNHDLRFLLSRYPAAYQVDRTRGTNPIIQ
                     ELENLKDAGSLYGGIIYNKAPIVMRQLEQLAGEENLKRGLQEYLKKYSWGNARWDDLV
                     AILEKAAQKPLGEWSRMWVKEAGMPVIAPVITEEEGYKIFFHEDDPAGLLRHWPQTLK
                     TMVITATDTVTVDMEPADRDSRVTTADQPLCIIPDISGRAYGTFLLDSLSAGHLLSEL
                     PGMHDPLLKGIIWINLNENLINGSIRPADFYSAAYSDLQGITDLQLRNYVSGRFSYVW
                     WNWLTAEERYELAPEAEAMLGNKMTTTESPAGRRTWFSLLRNVTVTPEGMKYLTSIWK
                     RGELPGTVTPGRKRSDPVKLSEDELCTLALTLALKNSPDAGSILAQQRERITGRERME
                     RFDFVTPSVSNDETVRDAFFESLSDPSNREREPWVLEALGYLHHPMIAERSEKYIQKS
                     LEMLEEIKATGDIFFPGNWVSTTLAGHSSPEARATVEKFLDDHPDYPADLKLKILQAA
                     DRLMRQ"
     assembly_gap    16048..16147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16276..19392
                     /locus_tag="PLA17_01645"
     CDS             16276..19392
                     /locus_tag="PLA17_01645"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPK84147.1"
                     /translation="MKKMILILLVAGLAGTICGQSHSAFLSDPAISPDGSKIAFVYES
                     DLWMADRNGGTAHRITAMTGEESVPRFSPDGKWLAFASTQNGNADVYIMPVDGGTLQQ
                     LTWNDGNDYPDSWSWDSKTIYFTSDRFNTFGTYTVPATGGTPARLFSDNYFDQAHHVV
                     EIPGSNGQECYFTTSWESYMFPHRKRYRGENDPDIEFYSKSTGDYKRLTEWEGKDMWP
                     SVDRNGKLWFASDEGNGEYNLYTFDNGAKRQMTSFSTSIRRPQVSADGRFVVFARDYR
                     IWTFDVATGQSSPCEINVWSNENLATEIGYNSAGKITDFDVSRDGKKIAFVSRGRMFV
                     SDITGKFIKEMPTDRGERVQEVRWMKDNESLLYTRTVKGWTNLFTITASAPAAEKQIT
                     RYERTLQNLLVSPDGDKALVNSGDSHIDLIDLKNFNVSGIIKDEFWFRPTRPRFSPDS
                     RYILYSAYRNFEPDIFIYDTREATTHTITSNGVAESDPCWSPDGRYIYLSADRYNAGF
                     PRGGGVSKLYRIPLYRFSESLRTDEYDKLFAKKASKDSLKVDIKIETDGIADRWEQID
                     VKGNSQSSPHVFNVRGKTMVLFNNSPNPRERVLTKVELSPFDPPKSAAIGDKGFSRLI
                     MTGDKFYALISGDVHEVKPAEGKAEKITLSATFSKNLHDEFVQMFYENWATLAEHFYD
                     LNYHGVNWKEMLSRYEQYLPLVRNRDNLRSIQNDMLGELNSSHLGFSSSGEEARLFYR
                     LQTNATGIIFSNDDPCVVEGWVKRSPADLTETSLRGGDRLVAVNGIRVEASVDRDKFF
                     ALPQLEKEITLRFDRKGEEYDMTLRPVSSAALKTLLYDSWVAENRRIVDSLSGGRVAY
                     VYMKDMGTSSLEKFLIDMTGIALGKEALILDIRNNRGGNVHDDVIKFLSQRPYLEWQA
                     RNGKRSPQPNFAPSGGPIILMVNEQSLSDAEMTAAAFRELKLGTIVGTETYRWIIFTS
                     SRMLVDGSSTRLPAWGCYDLEGNDLEITGVKPDIYVRTTFGDMQKGADPQLVRAVEEA
                     VKGIRSRQ"
     gene            complement(19601..19969)
                     /locus_tag="PLA17_01650"
     CDS             complement(19601..19969)
                     /locus_tag="PLA17_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPK84148.1"
                     /translation="MGSYVNGHLIKDEIVVYETKYHWVIFFSLKSIFTLTLYAWFKRW
                     LAEFVITNRRIVIKEGFIARRTFEMNLSKIETVNVDQSVMGRILNYGSIIIIGTGGTT
                     ERFHNIARPMVFKRRFQEQC"
     gene            complement(19999..20385)
                     /locus_tag="PLA17_01655"
     CDS             complement(19999..20385)
                     /locus_tag="PLA17_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HPK84149.1"
                     /translation="MPRVFKALEIEKEEKEIMRDYLDRHMPHVICPDTVKRGEKFKVK
                     VRMGDEYPHPDDDDHFISLIQLWNRETLIAEARYSAGANGHRPIHVEVDFYLVAPEVS
                     MNLSAMAVCTKHGLWQSEDKAVKVTN"
     gene            complement(20849..21520)
                     /locus_tag="PLA17_01660"
     CDS             complement(20849..21520)
                     /locus_tag="PLA17_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84150.1"
                     /translation="MKYISKLAAACLMILAVTDLCHSQESQSVNMAQYLFPEFTECII
                     KSKTGSVTTETGNYNTITGKMAFMKDGVIYGLTTARQADTITIDNRHFVPFGEFIYEV
                     VVNAPITLFIQHESRLIPPGQQVGYGGTSKVSKVNTYSHMSTQGGVYSLEIPPDFEIK
                     YLPVYWVRHNGEMHSFLGRARFIKIFPEYKKEIGSFIKSQGIKMEDREDLIRLVKYCN
                     ELYAQ"
     gene            21723..22235
                     /locus_tag="PLA17_01665"
     CDS             21723..22235
                     /locus_tag="PLA17_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84151.1"
                     /translation="MKNILIIGLIFLVSMVSCSKEDSDADLLYGNWTLESDSGYPAPR
                     VEFNTKGDYCMVDYTNVSFVGTTFCGYEITGEFSINGNQITFHTAVVERLEDGTGETE
                     FPYEVAITGSSPFGSFYFGLSFVSTLPDGHFSGVLNLQFEPVVWEIITLTDNTLEVKI
                     GTHTFRFVRR"
     gene            22310..22381
                     /locus_tag="PLA17_01670"
     tRNA            22310..22381
                     /locus_tag="PLA17_01670"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22342..22344,aa:Met,seq:cat)
BASE COUNT         5481 a         5519 c         5384 g         5904 t
ORIGIN      
        1 gggttacaga gacaacccat ccctcctcgt cagcggcctc aatggaggtg gtacccgact
       61 gaaatttact catgaaactg ctgtaccgtt ccggatcatc agacggatag ataatacggg
      121 catcagcctg ccatgaatcg agcagcgtgg agaaaggatt caccttcccc tcgaagggat
      181 agggatcacc cggcctgaca gccggatcat tgcgatcagg gttgataagc ttgctccgct
      241 cccttgcata atctttggag agaagaccct gcatcggctc ttcgggcggg aaggcagggt
      301 caccataata gaagtccctg tcggcaaaag ccaggttcat tgcctggtaa agggtatgga
      361 tataccttgt ggagttgtag cccatctcct tcaggttgaa gttttcaagt atgttcaggg
      421 tctggagcat caccgggccc tgggtccact gctgcagctt atagacttca atgcccttgt
      481 actcagtcat aagcggttcc tcaatcttaa cctgccagcg ggccaggtct tcctttgtta
      541 tcagtccgcc ctgctcctgg catccacgca caaactcatc tgcgatgtca ccgttgtaga
      601 aacggtcata agcagcatag atggcatcac gtcttgattt cttcttcttc agcgcctgct
      661 gttcggcctc taccagtttt gtcagggtag caagaagctc cttctggaca aacacttctc
      721 cggcagaggg tgcctcccgt tcttccccgg catgcgggag aaacaccttc gctgaatagg
      781 gccattcctt tatcctgttt tttgcacgct caattgaatt tgctgtctgg gcctctatcg
      841 ggtagccggc agccatctcc atggccgggg caagaacctc tttcaggctc atggtgccat
      901 actcggccag catcaccatc agtcctccgg gtgtgcccgg cgttactgct gctagcggac
      961 catactcagg cgggtatttg taacccttcg atttgtaaaa ttccgctgtc gcgcctgttg
     1021 gggccactcc aagggcattg attgccagca cctttccggt cttcggatta tagatcagcg
     1081 cctgggtttc acctccccag ctaagtacgt cccacatggt gcatgtagct gccagcatcg
     1141 cgcatgcggc atccactgcg ttgccacccc gatcaaatat cattgcccct gccgtggcag
     1201 caagaggttt gccggtgatg gccatccagt gacggccatg cagcggtggc ttctgggtgg
     1261 taaccggaaa tgtgttgaat ggctgtgcac cgatcaccaa cggaacacac aggaggagtg
     1321 ctgagataag ggtcttttcc attacatttt tgggcattat tgttggtcaa ggttcgtcat
     1381 cgccacaggc aagttcgcaa ataattacaa taaagaacga aaaatgttat tgcaaccatg
     1441 acaaatgttg tcagttttat aattgaagca gcatacttta gcgcctcaaa ataatgagta
     1501 ataatgatta ataatgagta aaatgaatcc ggagttatat ttttggtttg ccttcatttt
     1561 gattttcgtt gtaggctttt gggtggatat gcgtgctgcg aagcgcaggc atgcagaaga
     1621 gagcgttagc accgcactca aatggacagg cttttggatc agcattgcat ttcttttcgg
     1681 gctggccatt ttactgttct ttccccagaa tgaaaacagc acaatcagca ccaggagcat
     1741 gatgggagta aaattttact ctgcctatct cactgagtat atgctctctg tagataacct
     1801 ctttgttttc atcatgattt ttaccatgat gagtgtacct ggtgagatac aacctattct
     1861 tctgaagtat ggtatattgc tttcgatatt cttccgtacc ctgttcatac ttataggaat
     1921 ggggctcata aacaggtttg agtggctgat ctacctgttc ggagctttcc ttatctacat
     1981 gacattcaag atgatgaaga gccatgatga ggatgagacg attgatccgg agaagaatac
     2041 actgatacgt gcagcaaaaa aattattcca ggtagatgtc aacaacaagg agcctaaatt
     2101 ccattcccgt gtcaacggta agtttgcgat cactcccttt gccctgacct tcctgttgat
     2161 aggatcaacc gatgtcatgt ttgccattga ttccgttccg gctgtgatag gggttattcg
     2221 cgagggaaac catgcactta cacttgaaga gagcaacttc cttgcaatct catccaacac
     2281 gtttgccctg atgggtctta tctcactgtt cttcgcacta aagggaataa tgaaaatgtt
     2341 caggcacctc aagacaggaa tctgcattgt ccttttcttt gtgggagcaa aaatgcttct
     2401 tgccggcgtc gattcagtca acatgttttt tgcaagacac tcatgggtat ctctggcagt
     2461 aatcctctca accctggcgt tatcaattct ctattccgtg gtggagacca ggataattga
     2521 aaaacgtgag gcaaagcttg caactggcaa tggcactgtc gacaccctcg acgcccttga
     2581 ggacaaaaaa gaagaatagc cgggcagata cagctaacta tttcaactga aataatcagg
     2641 gaacagggat tgatgtttct gctgaaatga tcagtgaact ggggtgggta ttcctgctgc
     2701 aggcattatc atttttccac ccgatacggc attgtttaaa caaaacaggg ttttggttgt
     2761 tgaatagccg aaccgggaag agagcagaac attaaatgaa tgaatttgac ataaaggcgg
     2821 cggaatggga caaaagccag atgcgccgtg aacgggcaga ggctgtagcc attgcaattg
     2881 accgccagat accccttgac agaaacatga cagccatgga attcggagca gggacaggac
     2941 tgctgagttt catgctgctg gataagttag gctctgttac cctgattgac agctcggagg
     3001 gaatggtgaa tgtcgtgaga gaaaaactgt ctggttcgtc tgctgcctct gcgatgaagg
     3061 ttgtgaaagc tgatctggag aaggaggagt accctgatga aatctttgac ctgatctata
     3121 cgctgatggt tcttcatcac gtcaatgatg ttgaaaagat aatctcgaaa ttcagctcca
     3181 tgcagaggtc cggcggatat cttgccattg ccgacctgaa cagtgaggac ggatccttcc
     3241 acgggaatgg ctttacgggc cacaggggat ttgaccccgg ttatctggca accatccttg
     3301 aaagacacgg ctatgccggg ataaggcaca gtaccgttta caatatcggg aaaactctcc
     3361 ctgatggcac cagaaaagaa ttcggggtat tcctgatcac ggccgtgaag aaacaagtat
     3421 taccagcaga ctaaatcacc cgataaaaca aaaaaaacaa taatatggct gacattgaaa
     3481 ttacattcga cggcggcaaa gtgattaccg cccacgtaaa cggcaaaact attaaaaccg
     3541 atcagtcggt aaagcatggt ggcggcggca ctgcccccga gccatttgaa ctgttccttg
     3601 catcaatcgg aacctgtgcg gggatctacg tcaaatcgtt ctgtgaccag agggggatca
     3661 atggcgaagg catcaaaata attcagtcgg ttgagattga ccctgacaag agggtgccct
     3721 cccggttcag gctgaatatc cagctgcctg cggattttcc tgagaagtac cgcacagcag
     3781 taatcaacgc ggcagagctc tgcctggtaa aaaaaaccat cagcaactca ccgcagttcg
     3841 agataacatc ttcagtcagc tgaggtgacc tcctgccggg atagtcaagg cagattgtgt
     3901 ccattataca attttaaact cccggctgaa gcgaccacct gcaggggcgg tctccgttac
     3961 tgccccctcc aggcctgatt tcttcctttg ctccggttgt aacaattgct atattagccg
     4021 ggaaagcttg tgctgatggt gcatccctgc atgctttaag gttgcaacaa ttgctatatt
     4081 agccggaaaa ctatcgcttt atgacagaga gaatgaagga agtgaaagcg tcagatttga
     4141 ctgacaacct gatcaggctt attgcatctg actggatgct ggttaccgcc gggaccaggg
     4201 agaaattcaa caccatgacc gccagctggg gagggatggg ccatctgtgg aacaggaacg
     4261 tggcatttgt ttttgtacgc cctgaacgct atacctatga tttcatagag agcgcggaag
     4321 gttttacact gacctttttc gatgaaaatt accgtgatgc tctcaacctg tgcggaacga
     4381 agagcggcag ggattgtgac aaggtggcag aagctggatt gacgcccttt tttaccggtt
     4441 caggtctgcc ggcatttgct gaagcaagga ttatcattga gtgccgtaag ctctactccg
     4501 cccagctcac cagggaggca ttcattgata gtgagcccct gaacaaacat tacagtgctg
     4561 aagacaaact gcacaagatg tatatagcag agattgtaag ggtttggaaa aaataacccg
     4621 gaacctggtc tcttcttagc gttacattac tctcaggtaa aactggcttt aagtagttga
     4681 cccttaacaa gtcttgtttt tcaattattc tgtacagtgg catatcttac tgacgaactg
     4741 ccggtcagta aatccaggac aaaataaggt gagatacttt gacagtaaca taagaatgaa
     4801 aaccgttctg gtttgtttct tctgcaactg acggatattg gcacgccctg cagcacggta
     4861 aggaaaagca accgggagcc aaagcattgt cgcagaaccc gctgccatgc acacccgacc
     4921 gaactctatc tcagcggatc agggctgttt gccatgttca caacggtcac aaggagaagt
     4981 tgcgccaaat cttccataat ctccggcttg atgatatcaa tgttatcaag aggaatatga
     5041 tagtaatttg ttgagccgta ggttgaaaaa ctgagagaag gcactccggc gccaataaac
     5101 ctggcagcat caaggcgtgg cctcccgaga ttggagaaat agctggttga aagcggtctg
     5161 tgaatgtatt tattgtttgc ttcttcaacg aatgaccaca gtttcgggta attggtgccc
     5221 gcagcagccc ttattgaatg gcctacaccc acaccgtcca tattgagcag gacactcttt
     5281 tcaagcggca taaccggctg tttgatatac tcataggcgc ccataattgc ctgctcttca
     5341 gcgccaaaag agagaaacat aactgacctc ttgagaggta tcttgtttgt ggccaatgct
     5401 ttggcaacac ccatcatcac ggctacggca gaagcattat cattggcgcc tggtatcatc
     5461 tcataacaca gcccgaggtg atcaaggtga gcacctatta ttatggtttc atccttcagc
     5521 acagggtcgg tacctttcag tatccctatg acattgcttc ctttgccatc aggaaaatgt
     5581 ctggtactca ttttaactgt tacaaccttt tttgtgttga atgaattggg tttgagtgtt
     5641 gaattgatct gcctgaccac atccctgtga ttgtatccgg tgccggcaaa gatgtccctt
     5701 acaaccgtgt cacctatgtg gacgtagaca aaatcttcat aataatcatt gttcggattg
     5761 gctatcgggc cataattata tatcatccct gcagcgccat gcttaaccgc gtttgcaagc
     5821 ttatgctggt gaaacgaata ctcataccag ggcatgaatt tttcctcccc ggctgaagga
     5881 cccacaggtg attcacgttc tacaagcaca attttgcctt tcacatcaat attacggtag
     5941 tcgtcatagt tgagttcagg cgcggtgatt ccatatccgg cataaaccac ctcagctgtc
     6001 acctcgccgt tcccggaggt ggaacccggc atatattcag tcacgtaatt atagtgcttc
     6061 agaagaacct catccttttt gagcggtaca tgaagggtca atccgcaatc gggataaacc
     6121 attgtataag ggatatcaaa ccattgaaac catcctccct catcgcctgc cggcgaaatg
     6181 tcccagctct caaactcacc cgcgagccat tctgcacacg cctggtactc ggctgtgccc
     6241 gtcagtctgc cctggtactt ttcatcacac tgtatcatga catattcaag caggtcatgg
     6301 cttgagatag tgtgcatagc cttcaggagc gtctcctcgc tgatttcctg cgcccgcgaa
     6361 ggaaaaacca acagtgacaa tgccagtaaa acaagtagtt ttctcatatt atcaagttta
     6421 taaggttata tcatattaat ctggtgccat taattcccgg tgcctgattg tgacttttct
     6481 cctgaagcaa caatggggtg gctgccggtg tcttgagatc gggggctttc cactgaggca
     6541 ataatagggg ggctggcagt ggctgatcag agcttctttt catctgtgac aatgggatat
     6601 tcaactgcgc cgaacagagg gtgcctcatg gctgacatca ttactattct ctttaccatc
     6661 cggctgtagg aataattgtt cttctccgct gccagggcga agcttgtatc aaagctgatg
     6721 tcattgtttg gattaacatc aagcagatag aaaacaccat cacgcagtct gagatcgaac
     6781 ctggcgtaat cattgcagcc aaaacccttc catgcagcca gtaccttctc cccgagacgt
     6841 cttgacagat gcccgtccag gggagcgggg ataagcgttt caatcttctc ataatgctct
     6901 gagccgggaa tgaacttgga atcataagtg cagagcctct cccccggttc agtgaatgct
     6961 gagaagtcca tctcagccac cggcaaaatc tcgggcggat tgttgttcca gacagagaca
     7021 tgaaactcac ggccatcaat aaaatcttca accatggccg gttgattaag ctctttgttc
     7081 accagcaata tctgttccct gagcgaattg gtatcaaaca caactgactt ttcagtaatt
     7141 gtcaggctgc agtgctccct ggagggttta actatggagg gaaagagagt ccagctgcca
     7201 gcctcctccg gtgtgagcac tgctccttca ggcactggta tgccaaggct gattatccgt
     7261 tccttggtct tctgcttatc ataactcagg tctatcacct caggtccgtt cccggtatag
     7321 gtaaagcccc tgtctgaaag aatctcggtc actgtttttt cgctaaacgg gatgcccggg
     7381 agggcttcac aaagattgaa gacaatggta tcttcaggtg aatgtttctc aagtatgcat
     7441 tcaagatgag gattacttaa ctcctcaagg tatgtctcaa atccctcttt tgccagcgcc
     7501 tcagccaaaa cagtgttgga gttccgggta tcatttatct cagttgcatc ccagttgtga
     7561 tcaatgttat atatgacaag aaccttcaaa aaaatgagtt tgccacgaaa ctaaaagaat
     7621 ttttgaagaa tgtctgacat acgggctgat ttgttctgaa aaaaacgttg tttttcccgg
     7681 ctgtttgagc tatttgcccc tccagaggtt gggcagaaac tgataataga gcaggtgccg
     7741 ccaggtagac cagtcgtgcc cgccatgacc acccgtataa tagtggtgtt tgatgttatg
     7801 ttttacaagg gcttcatgca gagccaggca tctttgcccc ataaaaccat ttgcctcctc
     7861 agtgccctgg ccaacaaaaa ggtaatcaac cttctcgttt actttcggat cattcaggaa
     7921 gtgtgcgagc gacgtttccg catttatatc ccctgcactc aggatgccga aactggcaaa
     7981 caggtcaagc gaacgaaaac cgatcaacat actgtgccgg cctcccattg acaaacccga
     8041 tatggcccgg ccttttggta accggatcgt gctgtatgct ttatcaacca gcgggattac
     8101 atggttccgg agttcctttt caaaaatatc aaaggtaagt tcggtatgcc tgggatgatt
     8161 ccggtgaatt atctgattgt tgggaatggc aataatcatc ggcacagctt tcccttcggc
     8221 cagcaggtta tcaagaataa aattcgcccg tccgtcataa atccagttcg acgggagttc
     8281 accgcttccg cccagcagat aaagaaccgg atattttttc ttcggattat aagcgggagg
     8341 agtgtaaaca tagagttcgc gttcaccgcc agttacttcc gaataatata catggcgtgt
     8401 cactgttccg tgaggaacat tccgggcatc ataatatgcc ggcccgtcgc catgcacaat
     8461 cagctcactg taaggcggca tggctgtaaa ggcagcataa gtattattcg ggtcagcaat
     8521 ctgaatgcca tctacattga aatggtaggc atacatatcc ggtttgagcg gccctatcgt
     8581 gagcgtccag acaccatcgt caccctttgt aaaaggaata ggttccctgc caaggttatt
     8641 ggctgtgtgg attgcctcga ccggcagaac aaccttctgc gcattgggcg ctttcaaccg
     8701 gaaagtaacc atattgtcgt cgcgcacctc gggcgagttt acagaggcgc tgaacggcac
     8761 aaggttctct gtattcttct gcggattgta atcgagattc acgttagcct gctgcgccca
     8821 tgatccgggc ggtattaaca aagctaaccc ggtgatcaaa atcaaatgtt tcatcataag
     8881 acaataattt tgaatttaaa ggtatgatgt aacccacatg actgacaatt acataaatat
     8941 gtacttgtgt atctgataga tgcaggtttc atatctgttt tttaatcaat agatcggtat
     9001 attttaaaaa tgattattaa caactacctg attatcagga ttgatcattc tttaaagtga
     9061 gcactcgaat taattggttt tatatctgtc agcaagaatt tacaaatctg ttatttaata
     9121 ttatccggtt attatttcag cccgctgttc tttatgcgga aatagtcgaa agagacatca
     9181 aagggcgtgt ctggttttgt tgtatcagaa tcaaaccaga aggtactttt catatattga
     9241 gctactgtgc catcgcatac gatcaggcct gccctgatgt ccttcagcaa caaactggcc
     9301 gttccgagca actcccaatt tgtgccgtca agtgaataat aagccgtata gatacttcct
     9361 cttttctcca gcttaagact taatgcattg tcacctgtga cttcatttct gaggttgaag
     9421 gttgccagtg attttgcgat gccgttttct tccataacca ggtcgattgt gccgggttgc
     9481 acacccctga ccctggtagt tttgaccaca gccctgaaca tcagcttcac aaaattatca
     9541 tcgtcctggt aagccaggat tcctgcattt tccggctgtg acggtgatct ggaacatacc
     9601 agacgggttt cgattgtcca gtcgttgttg gcgctctgca gcaataaatt tctcgcatta
     9661 ttggtagcct ccaggacacc tcccggttca cctgtaattg tcagcgaacc tggttttacg
     9721 gacagactcc gggttgattt gttttcgcga acccatttcc accgggggtc aaccgtaccg
     9781 ccattgaatt catcgcttac tgattccgta tcaaaattca ggtagtagac attcttttca
     9841 agggtgacat tatcaatcaa ttgcactgtg gcagttcccg gtattgccct ggcctgttca
     9901 atctcaaccg aaactccgct gcccggagct tcagctttca ctacgggaat tttcgaattg
     9961 cttttcagga ggtaactgta cgcctttacc tctttatcga atcccggaat tcttttcccg
    10021 ttaactgtaa ccgatccggg attcaggtcc ggcataacct taaccgggta gctgtttgaa
    10081 acggttgtcc cgtcaattgt cacataagcg aaaatggatg ccacacccac gcctgtagct
    10141 gttaccctgc ccttttcatc gacagcagct acaccaggat tgttgctttt gaaggtaatc
    10201 cctgcttttg tgatgtcata gaaactgtcg tcagacatgc tggcagtaac acttgtctgc
    10261 acaacatctc ccggccttag caccaccctg tcacacccgg ccacaacagt tgtcaatacc
    10321 ggtttgtagg taccgctcat ccgcgcctcc acctctcctt ttatgtcgcg tgatgatgct
    10381 ccaatttcaa aaatatacct gcccggatcg aaagtaatct tccccttttc cgaatcccag
    10441 aaccaaaggt cttcacagtt gatatctatt gttactgttt ttacctgtcc gcgggggatc
    10501 atcacccttt tgaatccctt cagccgcttt atcggacgtt caagggatgc gtgactgtcc
    10561 ggggttttca cataaacctg caccacctct tctccgtcaa cagttccggt attctgaaca
    10621 tcaacactta aagtgatctt atcattcggt gtgatgctgg ttttgctgat tgcaaaattg
    10681 ctgtatgcga atgtggtgta tgacagaccg tatccgaact cgtaagaaac atctttattg
    10741 aaatacatgt aagtgcgccc gttggtgcca tcgccgcgca ggctgtaatc attaaaatca
    10801 ggcaggtcat tcagtgattt atgccatgtc acattgagct ttccgcccgg gttcacatca
    10861 ccgaacagta ttttcgccat tgccgtgccc tgtgcctgac cgttgtaacc tgtccagatg
    10921 atccctgtaa catcagcgag attcttgaac tgttccactt caaccattcc cattgtttgc
    10981 atcactacaa ttgtgttggg atttattgca gccaccgcct ttatcagctc attctgattg
    11041 cccggaaggg ttaacgcgaa acggtctgat tcctctctgc cggtggactg gtcggtgcct
    11101 acgaaaacca gggcgacatc gcagcctgct gccaggtcaa gtgctgctgc atcgatctcc
    11161 ttctctgctt cgcgatagac aagtacaagt gtttgcgggc cggtgatacc cagggtgttg
    11221 attttcaccg ataccaccct ggggcgtgcc caaccgggga atcctcccct gtccggaggg
    11281 ctgctgattc tttccgaggc cagtacattg ccggcggcag aaccaaccct gacctccagt
    11341 atgccaccat cagccgatga actcaggttg aaccttattg aatcaatatt tgtgatatct
    11401 atattatcat aggcagtcca gtcgccgtcc tttattccgc ttataacagt ccatccatat
    11461 cttgacgcta caatcaggcc gggagctgaa gagctgaatt gtgttgcatc gtattcctcc
    11521 aacacctctc cattccggac tgtcgaaaaa cggttcacgg ttaaaaagtc ggtctttctg
    11581 tcggtgtttc cgctaatcac ggatactatt tcggtattca ggttatgctc ctttatgtag
    11641 ttctggatcc cttccagcgg tgaaatactc agatgaggtt caacctctcc tgaataatcg
    11701 cccagctcaa ctttatcggc ctgtgggccc aatacagcaa tcttcctgac acgcttcagg
    11761 tcaatgggta aagctttgcc ccctgcttct gtgattgtgc tgtttttcag aagaacgggt
    11821 gttttggttg caacttcaag cgcaaggtca ttatgagaag ggtcgttgat tattcctggt
    11881 ttaatcccgg catagggaac aatttcacgg gggtcaaatt ctcccatcct cattctgatg
    11941 gtgaagaaat tcaccagcgc cctgtcaatc tctgcttcgg taagtattcc cagtcgaaca
    12001 gcttccaggg cattggcctg gtaaacgctg ccgcaatcgg tgtcaacacc tgcctttatt
    12061 cccagcgctg ccgccactac atggttttca gcataattat gccctctgac catatcatca
    12121 atcgcgccgc agtcgccggt tacataaccg tccaggccat aggttttccg cgcgatggta
    12181 tcaacaaggt atttgcttgc cgagaccggt attccgttta cagcgctgta ggctgtcatt
    12241 attgacggaa gcttataatc ccttataagg gttctgtaag gcaggagata gtattcgcgc
    12301 atatcgcggt cgtccatgtc tgcgctgcca ttgtgccggt tgaattccgt gttgtttgcg
    12361 aagtagtgtt ttccgcaggg gactgctttc aggtagacag gatcgtcacc cattaatccc
    12421 tgaataaatc ctttaccgat ctgagaaaca agaaaggggt cttccccgaa agtctctgca
    12481 gtacgtcccc accgcgggtc acgggccggt tcgatgacag gcgaccagta tgtaagtgtg
    12541 aatatcagat tgtggttaaa tccccgggcc tcatcggaaa tcacctttgt ctcgcgtttg
    12601 atcagctccg ggtcccaggt cgccccgacg gccacgctgt tggggaaaga agttgcagtc
    12661 atgccgctgt tgttgtttct gcccattatt ccatggagcg cctcgcccca gatgtcataa
    12721 tgatttaccc ccaggcgcgg aatgggggcc attgtattgc ccaactggct ctgcttctct
    12781 tcgggggtca ttctggaaac aaggtctgca gccctttcct taaatgaata cgcgggattc
    12841 agatagattg gcttctttgc tttttcgctg cctggcataa aagccattat caggatagag
    12901 aagagtacca ataggaacag ttgatttcgc ctggttttca taggtaaagc attttttata
    12961 tttgtagttc aatctgagtg catacaccgg ttgccttcat tatattccac cggaaaaaat
    13021 gataaataat tctgtgtcaa cagaacatca aaccatcccg aactgtgagc ctacaatttg
    13081 caccggcgtg aggttggctt tggaaccggc ttttgcataa ggaataagct cctctttaac
    13141 gggatttctc cacacaaatt taagaaaaaa agagagaagg aaaccggttt gccttactgt
    13201 gagctgattt atcgtgacga atgttttaca acatgcttcc tgccacaggc agagagttct
    13261 gcataaagat cttcaactgc ccctgctgcc tcatcaacct gtcagctctg cctggaggac
    13321 cttcaactgt ctctactgcc gcatcagcct gtcagctgcc tggagaatct tcagcttcag
    13381 atcagccggg taatcagggt gatcatcaag gaacttctca accgtggcgc gggcttcggg
    13441 agaagagtga ccggcaagag tggttgacac ccagtttccc gggaaaaaga tgtcaccggt
    13501 agccttgatc tcttccagca tttccagcga tttctgaata tacttctcag acctttctgc
    13561 tatcatcggg tgatggagat agccgagtgc ctccagcacc cacggttccc gttcgcggtt
    13621 ggatggatcg ctaaggctct cgaagaatgc atccctgact gtctcatcat ttgacactga
    13681 cggtgtcaca aaatcaaacc gctccattct ctccctgccg gtgattcgtt cgcgctgctg
    13741 cgccagaatg ctgccggcat caggactgtt cttaagtgcc agggtcagtg cgagggtgca
    13801 tagctcatcc tcgctcagtt tcacaggatc agaccgtttc ctgcccggag tcacagttcc
    13861 gggaagttct cctcttttcc agatggaggt gaggtacttc atcccctccg gtgtgacggt
    13921 cacattcctc agtaaactga accatgtccg tctcccggca gggctttccg ttgttgtcat
    13981 tttatttccc agcattgctt cagcctccgg tgccagctca taccgctcct cagcggtgag
    14041 ccagttccac catacgtatg aaaaccgccc tgacacataa ttgcgaagct ggaggtcagt
    14101 tataccctgc agatcactgt atgccgccga atagaaatca gccggcctta tactcccgtt
    14161 gatcaggttc tcattgaggt ttatccagat gatacccttc aacaacggat catgcatccc
    14221 ggggagttca gacaggaggt gcccggcact caggctgtca agcagaaatg taccataggc
    14281 ccttcctgaa atatccggga taatgcacaa cggctggtca gcggtggtga cccgggaatc
    14341 gcggtccgcc ggttccatgt caacggtaac cgtatcagtt gcagtgatta ccattgtttt
    14401 cagtgtctgc ggccagtgcc tgagcaatcc tgccggatca tcttcatgaa aaaagatttt
    14461 gtatccctct tcttccgtga tgaccggtgc tattacaggc atacccgcct ctttgaccca
    14521 catcctgctc cattcgccga gaggcttttg cgcggccttc tcaagtattg ctaccaggtc
    14581 atcccaccgt gcattgcccc atgagtactt tttcagatac tcctgcaacc ccctcttcag
    14641 attctcctct cctgcaagct gttcaagctg gcgcatcact atgggggcct tgttgtagat
    14701 gatgcctccg taaagtgacc cggcatcctt caggttctca agctcctgga ttatagggtt
    14761 ggtgccgcgt gtacgatcaa cctgataggc agcagggtat cgcgagagaa ggaaccgcag
    14821 gtcatggttg acatccggaa aatcaggtcc ggcaatcttg tccgacatgt agcctgcaaa
    14881 cacctccttc agccatacgt cgtcaaacca cttcatcgtc accagatcac caaaccagat
    14941 atgcgatgtc tcatgtgcaa tcagcgaggc ccgtgaaagc ttttcgttga gagtgggaga
    15001 agcttcaagc agcagggaag aagccctgta aaatattgaa cccgggtgtt ccatgccgct
    15061 gtactgaaac ggcggcaata gtacaaaccc gaacttgtca aagggatagg gtatctgagt
    15121 gtactcctca agccacttca gggcactgta atgaagccgg aatatctcat ccgagttgtt
    15181 ctcaacataa ccggggcggt tttcgcggtg cagcatctcc atcttcaccc cctcaatctc
    15241 cttctccacc agctcaaatc tgcctgcggc gaaggcgaag agataggtgc tgatcggctt
    15301 tgtttcgctg aagcgaaggg taaccctgcc atcagtggtg acagccgaaa cgatggggtt
    15361 gtttgacatc gcccggtaag attccgggat atccagtgca agggtgaaac ggcctttcag
    15421 gtcaggctgg tcaaagcatg ggaatgctgt gcatgccctg tcggggacga acagagtata
    15481 gaggtattca tcgtttctgt tcagtgagag gtctcctgca cggaaactca cctcaacacg
    15541 gttgtatctt cgtgagagca gaccacggtc aatcactata tgaccgttgg tcagcgccgc
    15601 ctcaacctct ctgccgttca ctgaaactga cctgaggtga tctccgggca ccctgaagtc
    15661 gagcaccagc ggctccctcc cggaccttct cacaaaatca atcataaccg tgccttcaac
    15721 cggagtactc ttccctgtat caatcgtgaa tatgagattg tacctgacat cacttatggt
    15781 ctcaaacctg taagtcgcca gaagtttcga gacaccttca gcggtgtatt cttctcggtc
    15841 tgcgcggttg catgaaacga gtgcagcaac ggcaatcaca acggtcagaa tgaagcctga
    15901 catgtaagat cgggttttca tgaaatctgt ttctggtcat caaatatagc aaatcggact
    15961 taaaggtttc cttaagcggc ctgcaatcct tcagtatccg tcatgagagc atgatccttc
    16021 attgttgcag ataaggcata tgtccctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnncat atgtcccttg tatatctaca gtgtcagtca tgagaaagtg cctgcatggc
    16201 aggttgctgc agaacattcc ggagggcaga gtgatattct ctatctttga attgttatcc
    16261 gttaattact acgccatgaa aaaaatgatt ctcattctgc ttgtggccgg gctggccgga
    16321 accatctgcg gacagagcca ctctgccttt ctctctgatc cggcaatctc tcctgacggg
    16381 tcaaaaatcg cattcgtata tgaaagcgac ctgtggatgg ctgaccgcaa tggcggcact
    16441 gcacaccgca taacagcaat gaccggagag gaatctgttc cacgtttctc tcctgacggg
    16501 aaatggctgg cttttgcctc cacacagaac ggcaacgccg atgtctacat catgccggtt
    16561 gatggcggca ccctgcagca gctgacctgg aacgacggca atgactatcc tgacagctgg
    16621 tcatgggatt caaaaacaat ctatttcaca tcggacaggt tcaatacgtt tggcacttac
    16681 acggttcctg caacgggagg aacccctgcg cgactcttct cggacaacta tttcgaccag
    16741 gctcatcacg tggttgaaat cccgggaagc aacggacagg agtgttactt tacaacttcg
    16801 tgggaaagct acatgttccc ccacaggaaa agatacaggg gagagaatga ccctgacata
    16861 gagttttaca gcaagagcac cggcgactac aaacggctga ccgaatggga aggaaaggac
    16921 atgtggcctt ccgttgaccg caacgggaag ctctggtttg catccgacga gggcaacggt
    16981 gaatacaatc tttatacttt tgacaacggt gcgaagcgac agatgacaag cttttcgacc
    17041 tccatccgcc gtccgcaggt gagcgctgac ggcaggttcg tggttttcgc gcgtgactac
    17101 cggatatgga ccttcgatgt tgccaccggc cagagttcgc cctgcgaaat aaatgtctgg
    17161 tcaaatgaga acctcgccac ggagatcggc tacaacagcg ccgggaaaat caccgacttc
    17221 gacgtgtccc gtgacgggaa gaagattgcc tttgtctcac gcggcaggat gtttgtctcg
    17281 gatatcacag gcaagttcat aaaggagatg cccactgaca gaggcgagcg ggtgcaggaa
    17341 gtcagatgga tgaaggataa tgagtcactc ctctacaccc gcaccgtgaa agggtggact
    17401 aacctgttca caatcactgc ttcagcgcct gctgccgaga aacagatcac acgatatgag
    17461 cgcacactgc agaatctcct tgtcagccct gatggcgaca aggccctggt aaacagcggt
    17521 gacagccata ttgacctcat tgacctgaag aatttcaatg tatccgggat cattaaggat
    17581 gagttctggt tccggcccac ccggccgcga ttctcacctg acagcaggta tatactctac
    17641 tccgcttacc ggaacttcga gccggatatc ttcatttatg acaccaggga ggccactact
    17701 cacaccatca ccagcaacgg ggtggctgaa tcagacccct gctggtcacc tgacggccgg
    17761 tatatttacc tgagcgccga caggtacaat gcaggcttcc ccaggggggg cggagtgagc
    17821 aagctctacc gcatcccgct ttaccgcttc agcgagtcgc tgcggacgga tgagtacgac
    17881 aagctcttcg cgaagaaagc ttcaaaggac agcctgaagg tggatataaa aattgagact
    17941 gacggaattg ctgaccgctg ggaacagatc gatgtaaagg ggaattcgca gtcatcacct
    18001 catgttttca atgtcagggg caagacgatg gtgctcttca acaactcccc caatccgcgt
    18061 gaaagggtgc tgaccaaagt ggagctctcg ccttttgatc cgccgaagag cgccgccata
    18121 ggcgacaagg gtttcagcag actgataatg acaggcgaca agttctatgc acttatctcc
    18181 ggagatgtcc atgaggttaa accggccgaa ggaaaggctg aaaaaattac gctgtcagcc
    18241 actttcagca agaatctgca cgatgagttt gtccagatgt tttatgaaaa ctgggctacc
    18301 ctggcagaac atttctatga cctgaactat cacggggtca actggaaaga gatgctttcg
    18361 cggtacgagc agtacctccc cctggtgcgc aacagagata acctgaggag catccagaat
    18421 gatatgctcg gtgagctgaa ctcatctcac ctcgggttca gcagttccgg tgaggaggcg
    18481 cggctattct accggcttca gaccaacgcc acggggatta tcttcagtaa tgatgatccc
    18541 tgtgtggttg aagggtgggt caagcgttct ccggctgacc tgactgagac atcgcttcgt
    18601 ggcggcgaca ggctggtagc tgtaaacggc atacgggtgg aggcttccgt tgaccgggat
    18661 aaattcttcg ccctgccgca gcttgagaag gagattacat taaggtttga caggaaaggg
    18721 gaagagtatg acatgaccct ccggcctgtc agctcagctg cattgaagac gctgctctac
    18781 gacagctggg tagctgagaa caggaggatt gtcgacagcc tgtcaggtgg cagggttgca
    18841 tacgtatata tgaaggatat ggggacgtcg agcctcgaga agtttcttat tgacatgact
    18901 ggtattgccc ttggcaagga ggctctgatc cttgatatcc ggaacaacag gggaggcaac
    18961 gtacatgatg acgtcataaa gttcctttcg cagaggccct atctcgagtg gcaggcacgc
    19021 aacgggaagc gctcaccaca gcctaatttc gcgccgtcag gcggaccaat tattcttatg
    19081 gtgaatgagc agtcgctgag tgatgcggag atgaccgccg ccgcgttcag ggagctgaag
    19141 ctggggacaa ttgtgggtac ggaaacatac aggtggataa tatttacctc ttcgaggatg
    19201 ctcgttgacg gctcctctac ccggttgccg gcgtggggct gttatgacct tgagggaaat
    19261 gacctggaga ttaccggggt gaagcctgac atctatgtcc gcaccacgtt cggtgacatg
    19321 cagaaagggg ctgatccaca gctggtgcgt gccgttgagg aggcggtgaa gggaatcaga
    19381 agcaggcagt aggtttgcaa ttcctaatgg tctgcggcca ttcagtgtga tttaatcatc
    19441 tgtactgcaa tttattatgg ctcgcaggca ggttatgtgt gcagtgcatt aagcattgca
    19501 gatggcaagg atacgaaata ctgccatggc aatagcatta agagttgcgg atggcaggga
    19561 tgtactagcc tgccatggca gtaacactaa gaaatacaga ttagcactgt tcctggaacc
    19621 tccgcttgaa gaccatcgga cgggcgatgt tatggaatct ctcagtggtg ccgccggtac
    19681 cgatgatgat tattgaaccg taattcagga tacgtcccat tacactctgg tctacattca
    19741 ccgtctcaat ttttgagagg ttcatttcaa atgtgcgccg ggcgatgaac ccttccttta
    19801 tcacaatgcg cctgttggtg atgacgaatt ctgccagcca tctcttgaac caggcataaa
    19861 gggtaagggt gaatatcgat ttcagtgaaa agaaaattac ccagtggtat ttggtttcat
    19921 atactactat ctcatccttg atgaggtgcc cgtttacata acttcccatt gtttatactc
    19981 ttattaatct attatatatc agttagttac ctttactgct ttatcttcac tctgccagag
    20041 accatgcttt gtgcagacgg ccatggcact gaggttcatt gacacttcgg gtgcaactaa
    20101 atagaaatca acctccacgt gaatggggcg atggccgttg gcgcccgccg agtaacgcgc
    20161 ctcggcaatc agtgtttcac gattccagag ctgaatcaga cttatgaagt ggtcatcgtc
    20221 atccgggtga gggtactcat cacccatgcg gaccttaacc ttgaactttt caccgcgttt
    20281 gacagtgtca gggcagatta catgaggcat gtgccggtcg agataatcgc gcattatctc
    20341 cttctcttcc ttctctatct caagtgcttt gaatactctt ggcatgacat ttttgtttag
    20401 cacaaccagc cggactgaat ggtcagtccg ggagatgtaa aactaggaaa atttccggtt
    20461 tgttatcaaa ccgtgaatat ttcgtgaggg atgaaaccag ggtcagacgc tattacattc
    20521 tccatttcct gttccatggt tttaccgccg gggatggctg catggcatta tcacttgggg
    20581 tgacctctca tcacaagttt ttcccggtcc cgattgctga taaccgtagg ctctatcgct
    20641 ttgcgtgacc tctcatcatc ttttgttccc cggtcccggc cgccggagcc cctggcacca
    20701 aagttcactg tcgggacgac aaggccacct ggctccgaag tccacttttt agacgacaga
    20761 gcatctggca atgatgctca ccgttgagac aacagagtcc ctggctcccg gttcattata
    20821 ttccattgtt gcttgataat cagcagtatt actgggcgta cagttcgttg cagtacttta
    20881 cgagcctgat caggtcctcg cggtcctcca ttttaatgcc ctgtgatttt atgaagcttc
    20941 cgatctcctt tttgtattca gggaaaattt taatgaacct ggcacgtccc aggaagctgt
    21001 gcatctcgcc attgtggcgg acccagtaca caggcaagta cttaatctcg aaatcaggtg
    21061 gaatctcgag gctgtagaca cctccctggg tgctcatatg ggagtaggtg ttaaccttag
    21121 agactttgga ggtgccaccg taacctacct gctgaccggg agggataaga cggctttcgt
    21181 gctgtataaa cagggtgatt ggagcgttga ccacaacctc gtaaatgaat tcaccaaaag
    21241 ggacgaagtg ccggttgtct attgttattg tgtcagcctg tctggctgtt gtcaggccat
    21301 agatgacacc gtccttcata aatgccattt tacctgtgat ggtgttgtaa ttgcctgttt
    21361 ccgttgttac tgaacctgtt ttcgatttta tgatgcactc agtgaattcc gggaaaagat
    21421 actgcgccat gttcaccgac tggctctcct gcgagtgaca aaggtcagtg actgccagta
    21481 tcatcaggca tgcggctgct aattttgaga tgtatttcat aacttctgat atcctccttt
    21541 tcaattaaag ttacattctg ccggataatt ccgaaagtta acaattttaa agcggtcagg
    21601 tatggccggt gcgcctggaa taactatggt tctcgtctga gaggagaaaa acagtgacaa
    21661 taggcgctgc ttgcaaatta tatctgtttt tcttaacttt cgcagtcaaa ttcctgtaaa
    21721 taatgaaaaa tatattaatt attggattga tttttctggt gtcaatggtc tcctgcagca
    21781 aggaggattc cgatgccgat ctgctttatg gcaactggac acttgaatca gattcaggtt
    21841 atcccgctcc cagggttgaa ttcaatacca aaggtgatta ctgcatggtt gattacacca
    21901 atgtgtcatt tgtcgggaca acgttttgcg gttatgaaat taccggtgag ttcagtatca
    21961 atggcaacca gatcaccttc catactgccg ttgttgagag actggaagac gggacaggag
    22021 agacggagtt tccgtatgag gtggccataa cgggcagttc tccgtttgga agcttttact
    22081 tcggattgag ttttgtttct actcttccgg acgggcattt ttccggcgtg cttaacctgc
    22141 agtttgagcc ggttgtctgg gagataatca ctctgactga caatactctg gaggttaaga
    22201 tagggacaca taccttccgt tttgtcagaa gataaatgac agccggtcat ttgacaatac
    22261 agattatttt ttgttcttaa ggaaatagta tgtatctttg cacccacatt gcggggtgga
    22321 gcagtggcag ctcgttgggc tcataaccca aaggtcggag gttcgaatcc tccctccgct
    22381 acaaagaa
//
LOCUS       DAOPBK010000014        22054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2503_length_22054_cov_4.660121, whole genome shotgun sequence.
ACCESSION   DAOPBK010000014 DAOPBK010000000
VERSION     DAOPBK010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22054
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_22054_cov_4.660121"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="PLA17_01675"
     CDS             complement(<1..1528)
                     /locus_tag="PLA17_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPK84152.1"
                     /translation="MRTKLLMLFAVLGLMLSQSIYAQPKTVTGTVTDATTNESLPGVN
                     VIIKNTTTGTTTDYNGAYSISVAPGQVLVFTFVGYASQEVTVGASNRIDVRLSPDVEF
                     IDEIVVVGYGTQKRVNLTGAVATVNVEKTLEARPIADAARGLQGTMPGLSVVIPSGEI
                     GSDPLIKIRGQIGSLQGGSSPLILVDNVEVPSLQMLNPDDIESISILKDAASASIYGA
                     KAAFGVVLITTKKGARVEGVNVTYSGNVSFQNISKKMEMAQLEALEYSLLAAERIGNT
                     VTGAFWKVTREGYEKAVEWQEKWAGVVKPDDPMLYGRDWYVDADNYKIGLRTYDAYDY
                     MIREWTPTQLHNFSVNGKSGRTDYNISLGYLDQTGIIKPSKIDDFKRYNGSVKLGTEV
                     TDWLKVNVGMLYSKRNKRHAYSTSSTTADPWLYLYRWGPTYPFVTEDGLPTRNPAYEM
                     SAANTANQENNYTSVTGGLEITPLKDWKIIFDYTHSNLEFITKRPGTSFAARNTWSAA
                     V"
     gene            complement(1642..2787)
                     /locus_tag="PLA17_01680"
     CDS             complement(1642..2787)
                     /locus_tag="PLA17_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPK84153.1"
                     /translation="MKKRDFLKISGIAAGALLAGCKASPSDPSAGRIRGAVKRGGPLK
                     LSYKPYELQLRHVFTVATNSRTTTPVVLTTLEYDGVKGYGEASMPPYLGESHESVLNF
                     LSKVNMEQFSDPFLMEDILSYIDGVMPGNYAAKASLDIALHDLTGKLLGQPWFRLWGL
                     NPQKTPATSFTIGIDTADVVREKTLEAGAFKLLKIKMGRDNDKEMIDTVRSVTDVPVF
                     VDVNQGWKDRNYALEMANYLSERGALFIEQPMPKEQVDDIAWLTERSPLPILADEALQ
                     TVEDLLPMKGVYSGINIKLMKCGGMHAAYKMITMARQLGMKILMGCMTETSCAVSAAA
                     QLSPLVDWADLDGNLLISNDVYEGMTVVDGKITLPERPGIGIVEIKS"
     gene            complement(2854..3945)
                     /locus_tag="PLA17_01685"
     CDS             complement(2854..3945)
                     /locus_tag="PLA17_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPK84154.1"
                     /translation="MTEKLEAIAAEMVPQRAESICDVTLVVSEGGILTVKGETNLPEA
                     KSAVIEMLEESGVTFADSITLLPDPAVVEKPWALTSVSVCNIRTRPSHAAEMATQALM
                     GTPVRILNKRDGWLLVQTPDSYIGWTDDPLAELSDEELAAWKGSERLIYIGHTGVITD
                     SRGETISDIVFGVILVKTGSRGNYWTVALPDGRTGEVRKQESEDFRKWAESAGIEPER
                     LIRFARTFTGSPYLWGGTSTKGVDCSGLTKTVYYYGGVIMTRDASGQFRYGEEVSIDK
                     PCETLLPGDLLFFGRLRDGKPRITHTGMYIGDTEYIHSSGLVKVNSFDSTRSNYSAYL
                     LEILQGARRVTGFTPGKGLERVAQHGWYF"
     gene            4229..5485
                     /locus_tag="PLA17_01690"
     CDS             4229..5485
                     /locus_tag="PLA17_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPK84155.1"
                     /translation="MKSLGRTIISASVALLITFLSSCRATGQVVETGIEVMARNNFDL
                     LQGKRVGLVTNPTGVDRNLRSTVDIINSAPGVKLTALYGPEHGVRGEFPAGEYVESET
                     DQSTGLPVYSLYGKTRKPSPEMLNNIDILVYDIQDIGSRSYTFISTLGLLMEAAAEKG
                     IPVVVLDRPNPLGGIKMEGVVTRPGFFSFVSQFAIPYIHGMTVGELAMFLNGEGMLAN
                     GIRCDLHVVKMNGWSRKMMFEETGLHWIPSSPHVPHRDSPLYYPATGIAGELYAVSIG
                     VGYTIPFQTFAAEWINADSLASALSALKLPGVIFRPVHYKPYYSTLQGKLVHGVQIHL
                     TDPAKAPLSLLQFYIMQEAHRLWPDKNLFEICNPSRHQMFDKVCGSDEVRKAFTRAFR
                     VYDILELWNSDIPAFRARASKYFLYK"
     gene            complement(5686..>7914)
                     /locus_tag="PLA17_01695"
     CDS             complement(5686..>7914)
                     /locus_tag="PLA17_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84156.1"
                     /translation="PDLGLLTSLRFLEWVAQNPNGVISLPTGKTPEYFIKWTKYLLNN
                     WDSPEARKIREDHGLYLQEKPSLRGLHFVQIDEFYPIDPRQHNSFYDYVRKFYIEGFD
                     LDPAKALLINSEEIVLADGKHYSEVFPDNLIDLSLRNREAVGSLEKLQQESIFRIDNW
                     CTGFEKKIRALGGIGFFLGGIGPDGHIAFNTRGSDRNSTTRLTATNFETQAASAGDLG
                     GIEVSRTRLVITIGLGTITFNPDAVAIIFAAGDAKALVVRNALEHEPDNLYPATVLQR
                     MKNARFYLTAGAAVQLHDSVEKYFREGEWTFEKTERAVFELCQKIDKYAHNLVLEDLK
                     NDEYCSLIPGLSLNTVTEVIESTKRKIEAGLMTERNTTFLHTGPHHDDIMLGIFPAII
                     PQLRHITNKFHFSVLTSGFNAVTNTMLLELLEETQRLISVGMIQMLRYPDFFESGYKF
                     KWDKDVSHYLDKVADKDEAGKLRGICHRIVRAMVDIYRLKSKDDLIEQIRKNIEIIRN
                     SYSGEKNPPDIQLLKGMIREYEEELVWAHYGVLVRNIRHMRLGFYTGDIFTQQPDKHR
                     DVKPILEYLRQIEPDIISLAFDPEGSGPDTHYKVLQAIAEALRQWSSEKDLSGLRIIG
                     YRNVWFKFRPSEANVFVPVSLNSMAVLNQSFRDCYISQVNASFPSPAYDGPFSHLSQQ
                     VWVEQYKQVQLILGKNFFYENESPKIRATHGMVYYKEMPVDEFLQHARELEKSVEGVN
                     IH"
     assembly_gap    7915..8014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8015..10833)
                     /locus_tag="PLA17_01700"
     CDS             complement(<8015..10833)
                     /locus_tag="PLA17_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HPK84157.1"
                     /translation="MKQNPIFETLGKELEGDLYTDDIQRILYATDASAYREIPMAVTR
                     PASVSDIRKILAFARDKGVSVIPRGAGTSLAGQVVGPGIVMDISRYLNHILEFNPDER
                     WVRVEPGVVLAELNRYLAPYRLQFGPETSTADRCCMGGMLGNNSCGSHSLIYGSVRDH
                     ILEVDAILSDGSEVQFGALTTDEFREKCNGDPGLLETKIYRNLNEMLSDERNAGEIRR
                     EYPHPALRRRNNGYALDALLETDPFTGNGQKINVCKLLAGSEGTLAFTTSMKLNLLPV
                     TDDLTGLLCAHFSSLEDAVRANIIALKHNPAAVELIDDYILNCTKDNIEQNRNRFFVR
                     GDPKAILLIEITRPTVEEIERTAVAIEKDMTGAGFGYHFPLYTGADEMARVWELRKAG
                     LGVLLNIPGRRKSVQVIEDTAVLPDLFPGYIAEAAGILEKYGLNCAYYAHIATGELHL
                     SPLLDLKNPDDIRTFRGLAEDMAALVKKYRGSLSGEHGDGRLRGEFIPLMLGPHNYAL
                     LKELKKTWDPTGLFNPGKITDTPPITENLRYPAGERRCYPGLVFEFPEEEGLLYAAEK
                     CSGSADCRKSALIGGTMCPTFMATRDEDKSTRARANILREFLTRSEKKNKFDHEEIYR
                     VMDLCLSCKACKAECPSNVDMAKLKAEFLQHYYDIHHVPFRAWLIAWLPRLYVPGMMV
                     RPLTNLLTSLPLFKRIIGFSVKRPVPALSPVTLRRWERRHRRKNGKLSASDSDRSAGF
                     SGLTETRDVNRPENTAPPKGTVCLFADEFTNYNDSDIGIKVIMLLERLGYRVIIPRHV
                     ESGRTFLSKGLLRSARRIAVKNIMMLRDVVSDERPLLGIEPSALLAFRDEYPEIAGRA
                     LADDARRLAGNAMLIEEFICREADRGNISAADFTDRPAEILLHGHCQQKAIASTAPTI
                     RMLSLPVNYRVKEINDGCCGMAGAFG"
     gene            complement(12188..12694)
                     /locus_tag="PLA17_01705"
                     /pseudo
     CDS             complement(12188..12694)
                     /locus_tag="PLA17_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(12701..15172)
                     /locus_tag="PLA17_01710"
     CDS             complement(12701..15172)
                     /locus_tag="PLA17_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPK84158.1"
                     /translation="MKRVVLMLLLAVLAAGIARADSWIRINQLGYLPGSVKVAVYISQ
                     EPVAEKSFTVYDALTDKPVFRGAAVICDGAVWGMAAAARLDFSGLTTPGGYYIIYSRT
                     KSETFRLASNAYDGTADFILNYIRQQRCGYNPFLADSCHRYDGVIVDHPTLSGTPIDV
                     RGGYHDASDHLRYTATTANSVYQMMFAYMKSPEIYADNWDAAGAAGSNGIPDILDEVK
                     WGLEWLLKMNPDSGVMFNQVADDRDHRGFRLPNHDTVSYGHGPYRPVYFVTGKPQGLA
                     AFKNRTEGVSSTAGKFASVFALGARLFARSDSLFAGTMADRAADAFEFACTDPGATQT
                     ACNVSPYFYEEDNYVDDMELAAWELWKLTGNNWYLEQADYWGTLEPCTPWIEKDTARH
                     YQFYPFVNLGHAGLALSGTEYSGKYIEFMRKGLEIINSRTRDDLFQVKIPFVWCSNNY
                     VVAALTQCRLYREATGDGRFDYMEAALRDWLFGCNPWGTSMICGLPAGGDYPQYPHSA
                     VTVFLGETTTGGLVDGPISKAIHESLLWLTLLRPDPYAAFQGGRAVYHDDIGDYSTNE
                     PTLDGTASLSYYLSALEKEGRTGMAESADDVYDAHGAIIRRGAGEKKIHLVFSADEHG
                     EGLEHILNVLEDKNITGSFFLTGNFLRNRSFASAVRRMIAGGHYVGPHSDGHLLYIPW
                     ENRDTLLVTKQQFNDDLKRNIAALRKAGLKKEIPRWFLAPYEWYNSAISRWTEDMGMK
                     LVNFTPGTGTNADYTTPDMANYRSSDQLIQRLASFGESSPQGLNGAIILIHPGTVPER
                     TDKLWLRLEELIDYYTSEGYTFNRL"
     gene            complement(15174..16517)
                     /locus_tag="PLA17_01715"
     CDS             complement(15174..16517)
                     /locus_tag="PLA17_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPK84159.1"
                     /translation="MTGKREPEISVGIVTGSGLTFTLNGDFFAGDSRKMLPGVYTAVA
                     DAEGLLLMNEAGQVRAGDEITFTPSDFRSCSFTLHAVTIGVNFHWQRNEDQTFRGDLK
                     FIKQGGEVTAVNILPVEEYLVSVISSEMSATSSSELLKAHAVISRSWLLAQMDKTLQL
                     IDTGEKYRTSFVTDEEITRWYDREDHRDFDVCADDHCQRYQGITRASTNAVEEAVRKT
                     GGEVLMYGDFICDARYSKCCGGATELFENVWEPVNHPYLRKVIDAGAGARTTDIDLTD
                     EKNATGWITGNPEAFCNTTDRNILRQVLNNYDQETNDFFRWKVTWRQDELSDLVRERT
                     GIDFGRITSLEPLERGTSGRIIRLRITGDRKSMVFGKELEIRKVLSKTHLYSSAFIVE
                     KTEENGDTLFVLRGAGWGHGVGLCQIGAAVMGATGYTYRQILSHYFRGSEIKKLY"
     gene            complement(16514..17452)
                     /locus_tag="PLA17_01720"
     CDS             complement(16514..17452)
                     /locus_tag="PLA17_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HPK84160.1"
                     /translation="MTDYSQKVRDLFEGQLREWALARDNYSRLDGVMVRTLSFPGYDI
                     SVQFNPGRITSSAAKVDAKSIGARPCFLCEKNRPGEQRGIPFGDDYIILINPFPIFRR
                     HLTIVSQCHTPQRISGNFDTMLSLAQALPDYVIFYNGPQCGASAPDHLHFQAGNRGFL
                     PIEKDVENPSLCRVVDEKDGTDLLLWSSYGRGILTLRGSGAEALTATFSRFHDRLALL
                     EPDRPEPMLNILAYHDEERWTVHIIPRRVHRPQCYFAEGDNRILLSPGSVDLGGVFIT
                     PRGEDFDKISAEDVTNILNEVCLGEDELKILTQELL"
     gene            complement(17466..18935)
                     /locus_tag="PLA17_01725"
     CDS             complement(17466..18935)
                     /locus_tag="PLA17_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK84161.1"
                     /translation="MDKSLICFLPFSDTGSVTASIASLRSSGLVSRVFVVLPPGSRGL
                     PDGSEAGLITSEGFAATDALRKMAAAAVESDYVLTYSKGFPLDMGNHALARMKQVCGD
                     TGAGMVYSDYYEKKGDAIAPHPVIDYQEGALRDDFNFGSVILYSGKAFRDACARMDRE
                     YAFAGLYDLRLKISQKYPLIHIPELLYTEVETDTRKTGEKQFDYVDPRNRAVQVEMEK
                     ACTDHLRCIGGWLPPRFRDVSFDDDFEYDASVVIPVRNRVKTIADAIRSVLGQKTKYR
                     FNLIVVDNYSTDGTTEIIRSFAGDSRLVHIIPGRKDLGIGGCWNEAVFSDRCGRFAIQ
                     LDSDDLYIDDSVVTRIVDAFHEQQCAMVVGSYRMVNFNLDEIPPGLIDHREWTPDNGR
                     NNALRINGLGAPRAFYTPVLRKIRIPNVSYGEDYAVGLAISRHYRIGRIYEPLYLCRR
                     WEENSDAVLDVAKANAHNLYKDRIRTIELLARKKMLAGS"
     gene            complement(18946..21927)
                     /locus_tag="PLA17_01730"
     CDS             complement(18946..21927)
                     /locus_tag="PLA17_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HPK84162.1"
                     /translation="MMILIPGQQSAGQKASRAERVAVSKMEKWVSPLFEGAIPKQFII
                     DSLKVDEENREINIWFPVAASYIPVREETVTSLENSVRSALGRKFTGYRINMISNGFS
                     LESLVPNYFRNTMPVDDNRIIPGAEDPPVLIRRVNAVSPEKGLGGKHIALWHSHGYYF
                     DMPLDRWEWQRAKLFGSVEDMSVMAYVLPYLTPMLENAGAVVLLPRERDIQVNEVIVD
                     NDISTGASGFVLQIPGEPENAGRGFLLKDTLFNGDNPFMAGTSLKISSGSALYVPEIP
                     ERGWYGVSVSYPRVPDYKGKATVRVTHTGGVSEFIVDQSIGGGTWLWLGTFHFGTGAD
                     PLKGSVTISGMDGSAALLDAVRFGGGMGNVARRPAESMISNQWSLNAGSQQATADSLP
                     SAPREYSWKLSGKPRFLEGARYWLQYAGMPDSLVYTPNKGRNDYNDDYMSRAEWVNYL
                     LRRPDTTVSGGLGIPVDLSFAFHTDAGVTPDDSIIGTLGIYSTITNGGLFPDGTSRLA
                     SRDFTDIVQTQIVEDIRALFNPDWTRRAMWDRSYYEARKPDVPAMLLELLSHQNMADQ
                     RYGFDPGFRFHVSRAIYKGILRYLADAGGREYVVQPLPVSHLAIEPVEGRRVSIRWQP
                     VTDPLESTADPVSYRVYMRSGDDGFDNGTPVSGTTFVTELPDYNTVYSFRVTAVNDGG
                     ESFPSEELSVAVNPASDDIVLIVNGFDRVSGPAWFDRDGMAGVAWWDDRGVADRYNFI
                     STGDQYDFERTSPWTDDDNAGWGASYSNDEGRIIPGNTFDFTRVHGESVIAAGKSFFS
                     VSDEVFTGNDFDLSRWCVVDLLFGEEKTTTSAYWPDRKDFRIYTPEFLRTLERMQKAS
                     LPVFMSGSYPGTDLVMTNDTSVASLVKKTLHFMPRTGHAVRTGSVAATDKAAPAFTGR
                     FEFNTGITDKIYAAESPDAIEPAGRQSVTAFRYLENNTSAAVMYTGDVRSFVMGFPFE
                     TIISRKERDELMKQILDFLLK"
BASE COUNT         5239 a         5965 c         5245 g         5505 t
ORIGIN      
        1 gcactgcagc tgaccaggta ttacgcgccg caaacgaagt acccggtctt tttgtaatga
       61 attccaggtt ggaatgtgta taatcaaaga tgattttcca gtctttcagc ggagtgattt
      121 caagcccgcc ggtcacactg gtatagttat tttcctggtt ggctgtgttt gctgcagaca
      181 tttcatatgc cgggtttctg gttggcaaac catcctcagt aacaaacggg tatgtcggtc
      241 cccagcggta tagatacagc cacgggtcag cggtagtaga gctcgtcgaa tatgcatgcc
      301 tcttgtttct cttggaataa agcatcccga catttacctt cagccagtca gtcacctccg
      361 tgccaagctt gacagatccg ttgtaccgtt tgaaatcatc tatctttgaa ggcttgataa
      421 tacctgtctg atcaagataa cccaggctga tattgtaatc tgtcctgcct gacttgccgt
      481 taactgagaa attatgcagt tgggtgggag tccactccct gatcatataa tcataggcat
      541 cataagttct cagcccgatt ttataattat cagcgtcgac ataccagtca cgtccataaa
      601 gcatgggatc atcaggcttc acaacaccag cccatttttc ctgccattca acagctttct
      661 catagccttc gcgggtgacc ttccagaatg cgccggtaac ggtgttaccg attcgttcag
      721 cggccagtaa tgaatattcc agagcctcca gctgggccat ttccattttc ttcgagatat
      781 tctggaatga gacgttcccg ctataggtta cattcacgcc ctcaaccctg gcacccttct
      841 tcgtggtgat cagaaccaca ccgaaggcag cttttgcacc ataaattgag gcagatgcag
      901 catccttcag aattgaaata gattcaatgt catcagggtt aagcatctga agtgacggaa
      961 cctccacgtt atcaaccagt ataaggggtg atgaaccgcc ctgaagtgaa ccgatctgac
     1021 cacggatctt aataagcgga tcagacccaa tttcaccact tgggataaca acgctgagac
     1081 cgggcatcgt tccctgcaga cctcttgcag catcggcaat aggtcttgcc tccagggttt
     1141 tttcaacgtt taccgttgca accgctcctg tcaggttgac tcttttctga gtgccgtaac
     1201 cgaccaccac gatttcatca ataaattcga catcaggact caatctcaca tcaatcctgt
     1261 ttgatgcccc tacagtcacc tcctggctgg cataccccac gaaggtgaag acaagtacct
     1321 gtccgggagc gacactgatg gagtaagccc cgttataatc agtggtcgta ccggtggtag
     1381 tgttcttgat aatcacattg acgccaggca acgactcatt cgttgtggcg tcagtgacag
     1441 tccctgtcac ggtcttaggc tgagcataaa tgctttgtga aagcatcagt cccaaaaccg
     1501 caaacaacat taatagtttg gttctcatgt gattggattt agggttaatt agttagttat
     1561 tatataggta tatgtctgaa aacaacaaat tcatttctca atatgcagtt tatccttttt
     1621 ataaatgatg tactacaact tttaactttt tatctcaact attcctatcc cgggccgctc
     1681 aggaagggtg atcttcccgt caactaccgt cattccttca tatacatcat tactgatcag
     1741 caggttgcca tccagatcag cccagtcaac cagtggtgat agctgagcag cggccgaaac
     1801 tgcgcatgag gtttcggtca tacaacccat cagaatcttc attcccaact gccttgccat
     1861 ggttatcatc ttgtaggctg catgcatccc tccgcacttc atgagcttga tattgatacc
     1921 tgaataaaca cccttcatcg gaagcagatc ctcaaccgtc tgaagggcct catcggcaag
     1981 aatcggcaac ggactcctct cagtgagcca tgcaatgtca tcaacctgct ccttcggcat
     2041 aggctgctca atgaaaagag cacccctctc actaagatag ttcgccatct caagggcata
     2101 attgcggtcc ttccaaccct ggttcacatc aacaaaaaca ggtacatcag tcaccgaacg
     2161 gacagtatca atcatctctt tatcattgtc cctgcccatc ttgatcttca acagtttgaa
     2221 agcgcctgcc tccaacgttt tttccctgac cacatccgcc gtatcaatac ctatcgtgaa
     2281 actggttgca ggagtcttct gaggattaag tccccagagc cggaaccatg gctggcccag
     2341 aagcttgccc gtgagatcat gcagtgcaat gtcaagcgat gccttggccg cataattgcc
     2401 gggcataacc ccgtcaatgt agctcaggat atcctccatc aggaaggggt cactgaactg
     2461 ttccatgttg actttggaaa ggaaattgag caccgattca tggctctcac ccaggtaggg
     2521 aggcatggaa gcctcaccgt agcctttcac accgtcatat tcgagggtgg tgagtactac
     2581 cggggtggtg gtcctggagt tggtggcaac tgtaaataca tgtctgagct gcagctcata
     2641 aggcttgtag ctgagcttaa gggggcctcc tctctttacg gcacccctga ttcttcccgc
     2701 cgacggatca gaaggagaag ccttgcatcc ggcaaggagt gcaccggctg caataccgct
     2761 gattttaaga aaatctctct ttttcattta agtaagtcag aattataaaa ccagatagag
     2821 cctgcatgtc tgacctgcag tctccatgcc ctgtcagaaa taccagccgt gctgtgcaac
     2881 gcgttcaagg cccttgcccg gggtaaaccc tgttacccgt cttgctccct gcaatatctc
     2941 aagaaggtag gcgctgtagt tgctcctcgt ggaatcaaag ctgttgactt tcacgagccc
     3001 cgaggagtga atatactctg tatcaccaat gtacatgccg gtgtgcgtta ttctcggctt
     3061 gccatccctc agcctgccaa agaaaaggag gtcacccggc aggagtgttt cgcatggttt
     3121 gtcaatgctc acctcttcac catacctgaa ctggcccgat gcatcacggg tcataatcac
     3181 accgccataa taatatactg tcttcgtaag tccactgcag tcaaccccct tggttgatgt
     3241 gcctccccag aggtagggtg aaccggtgaa agtcctggca aagcgaatca gcctctccgg
     3301 ctctatgcct gcactctcag cccacttccg gaaatcttca gactcctgct tccttacctc
     3361 ccccgttctg ccgtcaggca gcgctacagt ccagtaatta cctcttgagc cggtcttgac
     3421 cagtatcact ccgaagacaa tgtcagatat agtctctcct ctgctgtcag ttattacccc
     3481 tgtgtgccca atgtatatca gccgttcgct gcccttccac gcggccagtt cctcatcact
     3541 gagctccgcc aaaggatcat cagtccagcc gatatatgaa tcaggtgtct gtacaagcag
     3601 ccagccgtcc cgtttgttga gtatcctgac cggtgttccc atcagcgcct gcgttgccat
     3661 ctctgccgca tgtgaaggcc tggttctgat attgcataca ctgaccgatg tcagggccca
     3721 tggcttttca accactgccg gatcaggcaa tagggtgatg ctgtcggcaa aagtgactcc
     3781 cgactcttca agcatctcaa tgacagcact tttagcttcc gggagatttg tctctccttt
     3841 aaccgtaaga ataccgcctt cagagacaac cagagtgaca tcacagattg actcggcgcg
     3901 ctgtggcacc atttcagctg ctattgcctc aagcttttcc gtcaattctg ccggaacctc
     3961 ctttttctgg caaccagcaa actgaaaaag aacgaataat aagacaaaac agggtaaggg
     4021 tttcataaaa gttgtattac atcttacaag tccaaaatta aatatttata atattcaagt
     4081 caatagacaa ttgaaattta tttaatttgt catacagaaa tcttataacc ggcagtgccg
     4141 ggatttgtat tatcagaatt gaaaactgcc tgttcaggcc ttgtcattcc tgccaggcaa
     4201 ccggtataga acataaaatg aacagtgtat gaagagctta ggaagaacaa tcatctctgc
     4261 gtcagtggca cttttaatta catttctttc ctcctgccgg gcaaccgggc aggttgtgga
     4321 gacaggaata gaggtaatgg ccagaaacaa tttcgacctc cttcagggga aaagggtggg
     4381 tttggtgacc aatcccacag gggttgaccg caatctgagg tcaacggttg acattataaa
     4441 cagtgccccg ggtgtaaagc tgacagccct ttacgggccc gagcacggcg ttcgcggcga
     4501 gttccctgcc ggagaatatg ttgaatccga gactgatcaa agcaccggtc ttcctgttta
     4561 cagcctctac gggaaaacgc ggaagccttc accagagatg ctgaacaaca tagatatcct
     4621 ggtctatgac atccaggata tcggatcaag atcatacacc tttatcagca ccctcggcct
     4681 gctcatggag gcggctgccg aaaaaggaat tcctgtcgtg gtgctcgacc gtcccaatcc
     4741 tcttggcgga ataaagatgg aaggagtggt cacccgcccc ggattctttt cattcgtcag
     4801 ccagtttgct attccgtata ttcacgggat gaccgtgggg gagctcgcca tgttcctcaa
     4861 cggcgaaggg atgctggcca acggaatcag gtgtgatctt catgtggtga aaatgaacgg
     4921 atggtcaaga aagatgatgt tcgaagagac aggcctgcat tggattccct cctctcctca
     4981 tgtcccgcac cgtgattcgc ctttgtacta tccggccacc ggcatagcag gagaactcta
     5041 tgccgtcagt ataggggttg gatacaccat tccctttcag acctttgcag cagaatggat
     5101 caatgctgac tcacttgctt ccgcactcag cgcactgaag cttcccgggg tgatcttcag
     5161 accggttcat tacaaaccct attacagcac tctccaggga aagctggttc acggagtgca
     5221 gattcacctg acggacccgg caaaggctcc gctgtcactt ttacagtttt atatcatgca
     5281 ggaggcacac cgcctgtggc ccgacaagaa tctctttgaa atttgtaatc cgtcaagaca
     5341 tcagatgttc gataaggttt gcggttctga tgaggtcaga aaagcattca ccagggcatt
     5401 cagggtgtat gacatcctgg agctctggaa cagtgacatt cctgcattca gggccagggc
     5461 ctcgaagtat tttctgtata agtgagtctg ccggcatgca ttggtcaggc ctgtggttct
     5521 gctactgtat gtcaccatgg tctgagctct aacaacaacg accctgtttc gcatactcgc
     5581 gcttagctcc tcctgtcacc actggcctga gttcccgtca gtggatatta acagtctgag
     5641 atcgtgggca ataagatagt caggcattat tgtctgagct ccacgtcagt ggatatttac
     5701 tccctcaacg ctcttttcaa gctcacgggc gtgctgcagg aactcatcaa caggcatctc
     5761 cttgtaatag accatgccat gcgttgccct gatcttgggg ctctcattct catagaagaa
     5821 attcttgccc agtatcagtt gtacctgctt gtactgctca acccacacct gctgtgacag
     5881 gtgactgaat ggcccgtcat aggctggact cgggaacgat gcattcacct ggctgatata
     5941 gcagtcacgg aacgactggt tcagcacagc cattgaattg agtgatacgg gcacaaagac
     6001 gttggcctct gacggacgga acttgaacca cacgttacgg taaccgataa tcctcagacc
     6061 tgacagatcc ttctcactgc tccactgccg gagtgcctcg gcaatagcct ggagcacctt
     6121 gtagtgcgta tcaggcccgg aaccttcggg atcaaatgca aggctgatga tatctggctc
     6181 tatctgtctg agatactcaa gaatgggctt cacatcgcga tgcttgtcag gctgctgggt
     6241 gaagatatca ccggtataga agcccagcct catgtgcctg atgttgcgaa ccagcacccc
     6301 gtaatgtgcc cacacaagct cctcctcata ctccctgatc atccccttga gcaactgtat
     6361 gtcaggcgga ttcttctcgc cactgtagct gttccttatt atctctatgt ttttccttat
     6421 ctgttcaatc agatcatcct tcgatttcag gcgataaata tcaaccatgg ccctgacaat
     6481 cctgtgacag attcccctga gcttgcctgc ttcatccttg tcagccacct tgtcaaggta
     6541 atgactcaca tctttgtccc atttgaactt ataccctgac tcgaagaagt caggatatct
     6601 cagcatctga atcattccta ccgagatgag acgctgtgtc tcctcaagca gctcgaggag
     6661 catggtgttg gtcaccgcat tgaaaccaga tgtgagtacc gaaaaatgga acttgttggt
     6721 tatatgcctc aactgcggaa taatggccgg gaaaataccc agcattatat cgtcatgatg
     6781 tggcccggta tgcaggaagg tggtgttccg ctcagtcatg agcccggctt cgatcttacg
     6841 ttttgttgac tctatcacct cagtgacggt gttgaggcta agtccgggga tcaatgagca
     6901 gtattcatca ttcttgagat cctccagcac aagattatgt gcatatttat ctatcttctg
     6961 acagagctca aaaaccgccc tttcggtttt ctcaaaggtc cattcgcctt ccctgaagta
     7021 tttctcaacg gagtcatgca actgaactgc tgcaccggcg gtgagataga acctggcgtt
     7081 tttcattctc tgaagcacag tggctggata gagattgtcc ggttcatgct caagagcgtt
     7141 ccttacaacc agcgccttgg cgtcaccggc agcgaagata atagccacag catcaggatt
     7201 gaatgtaatt gttcccagac cgatagtaat caccagacgc gttcttgaca cctctatccc
     7261 gccaagatcg cctgcagagg ctgcctgtgt ctcgaagttt gtagcggtga gcctggtggt
     7321 ggagttacgg tcacttcccc tggtgttgaa ggcaatgtga ccgtcagggc cgataccgcc
     7381 aaggaagaag ccgataccgc caagggcacg gattttcttt tcaaacccgg tgcaccagtt
     7441 gtctatcctg aagattgact cctgctgcag tttctcaaga gaaccaactg cctccctgtt
     7501 gcggagcgaa aggtcgatga gattgtcagg gaaaacctcg gaataatgct taccatcagc
     7561 caggacaatc tcttccgagt tgatcagcaa tgcctttgcc ggatcaaggt cgaagccctc
     7621 aatataaaac tttcgcacat agtcatagaa gctgttgtgc tggcgcggat caatggggta
     7681 aaactcgtcg atctgtacga aatgaagacc tctcagcgac ggcttctcct gcaggtacaa
     7741 tccatggtcc tccctgatct tgcgggcttc gggactatcc cagttgttca gcaggtattt
     7801 tgtccatttg atgaaatatt caggtgtctt gcccgtaggc aggctgatga ctccgttggg
     7861 gttttgggcc acccattcaa ggaacctgag tgatgtcagc agccccagat cgggnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgaat gctcctgcca ttccgcagca
     8041 cccgtcattt atctctttca ccctgtagtt aaccggaagt gaaagcatcc tgattgtcgg
     8101 agcagtggag gctatagcct tctgctgaca gtgtccgtga agcagtatct ccgccggccg
     8161 gtcagtaaag tccgccgcgg atatattccc ccggtcagct tccctgcaga tgaactcctc
     8221 aatcagcatt gcattgccgg caaggcgccg ggcatcatca gcaagcgctc ttccggctat
     8281 ctccggatac tcatcgcgga aagcaagcag ggctgagggc tctatcccaa gtaacggcct
     8341 ttcatcggaa acaacatcgc ggagcatcat aatgttcttt actgcaatcc tgcgtgccga
     8401 cctgagcagt cccttcgaga ggaacgtacg gccgctctca acgtggcgcg gtataattac
     8461 tctatagccg agccgctcga gaagcatgat aaccttgatg ccaatatcac tatcattata
     8521 gttggtaaac tcatcggcaa agaggcagac agtacccttt gggggtgctg tgttctccgg
     8581 ccggttgacg tccctggttt ccgtgaggcc tgagaagccg gctgatctgt cactgtcaga
     8641 agcgctcagc ttcccgttct ttctcctgtg gcgcctctcc catctcctga gtgtcaccgg
     8701 cgaaagcgcc ggaacaggcc ttttaacgga aaaaccgatg atccttttga acagcggcag
     8761 gctggtaagc aggttggtaa gaggtctgac catcatcccg ggaacgtaaa gacgtggcaa
     8821 ccaggctatc agccaggcgc ggaacgggac gtggtgtata tcatagtagt gttgcaggaa
     8881 ctcagccttt aatttggcca tatcgacatt tgaggggcac tcagccttgc aggccttgca
     8941 tgacaggcag aggtccatca cacgatatat ctcctcgtgg tcaaacttgt tcttcttctc
     9001 tgaccttgtc aggaactccc tgagaatgtt tgccctggcg cgtgtcgact tatcctcatc
     9061 acgggttgcc ataaaggtgg ggcacatggt gccgccaatc agtgcgctct tccggcagtc
     9121 ggccgaaccg ctgcatttct cggctgcata gagcagaccc tcctcttcgg gaaactcgaa
     9181 aaccaggccc ggataacatc tcctctcacc tgccggataa cgcaggtttt cggtaattgg
     9241 aggagtatcg gtaatcttgc ccggattgaa gagcccggtc gggtcccatg tctttttcag
     9301 ctctttaaga agtgcatagt tatgaggtcc gagcatcaaa gggataaact caccacgaag
     9361 cctgccgtcg ccatgttcac cgctgagcga gccgcggtat ttcttcacca aggcggccat
     9421 atcctccgcc agcccgcgaa acgtgcggat atcatcgggg ttcttgaggt caagcagcgg
     9481 gctgagatgc agttcgcctg tggcgatgtg ggcatagtag gcacagttga gcccgtattt
     9541 ctccagtatg cctgcagctt cggcaatata accgggaaag agatcaggca acaccgcagt
     9601 gtcttcaatc acctgcacgc tctttcgccg acccggtatg ttgagcagca ctcccagtcc
     9661 tgccttgcgc agttcccata cccgggccat ctcatcagct ccggtgtaga gcgggaagtg
     9721 gtatccgaac cccgcaccgg tcatatcttt ttcaatggcg acggccgttc tttcaatctc
     9781 ctcaactgtc ggccgggtga tctcaataag cagtatggct ttcgggtctc ccctgacaaa
     9841 gaagcggttc ctgttttgct cgatattgtc cttcgtgcag ttgagtatat agtcatctat
     9901 cagctccacc gctgccggat tatgcttcag cgcaatgatg tttgccctga cggcatcctc
     9961 caggctgctg aagtgggcac agagcaggcc ggtgaggtca tctgtcacag gcaacaggtt
    10021 gagtttcatc gaggtggtga atgccagagt gccttcagaa cctgcaagga gcttgcagac
    10081 attgattttt tggccgttgc cggtgaaagg atcggtctcc agcagggcat cgagagcata
    10141 gccgttgttg cgccttcgca gggcaggatg cgggtactcc ctgcgtatct caccggcatt
    10201 gcgctcatca gagagcatct cattcagatt cctgtaaatc ttcgtctcaa gtaatccggg
    10261 atcaccgttg catttctccc tgaactcatc ggtggtcagt gcaccgaact gcacctcaga
    10321 accatccgag agaatagcgt caacctccag gatatgatcc ctgacacttc cataaatgag
    10381 tgaatgggac ccgcacgagt tgttgccaag cattcctccc atgcagcacc tgtcagctgt
    10441 cgatgtttcc gggccgaatt gcagcctgta cggcgccagg taacggttaa gctctgcaag
    10501 caccactccg ggttcaaccc tcacccacct ctcatcaggg ttaaactcga gaatatggtt
    10561 gagatacctc gaaatatcca tgacaattcc cgggcctaca acctggccgg cgagggaggt
    10621 gcctgcacct ctcggtatca cagaaacacc cttgtcacgg gcaaatgcca ggatcttcct
    10681 gatatctgat accgacgccg gtctggtgac agccataggt atctccctgt aggctgaggc
    10741 atcagtggca tacagaatac gctgaatgtc atcagtataa agatctcctt caagctcttt
    10801 cccgagggtt tcaaaaatgg gattctgctt catatactgt tgacataatt tattttgaat
    10861 cctttttccc aaaagccgga tcaatcttca gcagggagac cgaaataatc ggaacaatac
    10921 tgcaaatcat cacccagatg aagaaatggt tgtaaccaag ctgttcctgg agccacccgg
    10981 caaacatccc gggcagcatc atcccgagag ccatgaaacc tgtgcagata gcataatgag
    11041 cagtcttgta ttttcctacg gagaagtaga tcaggtaaag catgtaagct gtaaacccga
    11101 agccgtaacc aaactgttcg ataaagacac aaatattgat aatcagcatg ttatctgtct
    11161 gggtaaagct caggtagagg aatgtgaccg aggtcagcag catgctcagg gtcattggcc
    11221 agagccattt cttcaagcct cccaccgcgg ctaccaaacc accgattatg ccgcccagcg
    11281 tcagcccgat tatccctaca gtgccgtata caagaccgac ctctccggtt gtcagtccga
    11341 gtcctccctt gtcgatcggg tcaagcagga aggggttgat aagcttaagc aactgagcct
    11401 ccgcgaaacg gaatgtcagc atgaagaaaa gtgcggcgcc cacctgtggt ttcctgaaga
    11461 aggaggcaaa agtgactccg aactccttca gcacatcgga ggccttcagg ttttcatgcg
    11521 gcctgtcagc gtcaggtttc gggagaacga tcctgtgata gaaactcaga acaaagaata
    11581 aaccggcaag cacaaagaag gtgactgccc aggccatcgg tatgtttcct gacagaccct
    11641 catatgttac tgaagtatag ctggcaagtt ttgagtcagc ctggaacacc atgtaagcag
    11701 gcttgttcca gtttgtttcg ttgaagacca ggtgttcgcc ctggataagg gcaatacttc
    11761 tatctccctt tttcagggct gtgttgagaa ccacctcttt accatgatca ggcttcccgc
    11821 ttatcctgac gggaacaacc gccacgcttc ctgttacgga ggctgcagtt cccctctcct
    11881 ctccgaagtt atccctgatc caggccccca ggggatccga gacattactg ctccaccatc
    11941 ccttctcctt cgccatcttc tcttcaagct tgtagaagcc gttgctgaga ttggatgatt
    12001 ttatcgagtc aagcaaaagg cccaggctgt ccttgtcaac agtctgagtg ctgatcatca
    12061 gtgaatcact atccagcaag aaatacaagt cacctggttt tgccgtattc tccgccacat
    12121 gcggcatcat cagagtagtt ttcgaatagt ttggtgaagc ctctatgaac agggttaccg
    12181 gttccctgcc ggaggagctc tcaaggaacc ctgcaagaat taccagaaca ccctgtccca
    12241 tgatagttgc aatacggtag aaagtgcttc ggataccaac atattttgcc tgctggtttg
    12301 tattgagccc cagcatgtag aatccgtcag cagcaatatc atgggtagct gacccgaatg
    12361 ccaggagcca gaagaatgcc agtgttattt tgaaaaagaa cgatgccggt attgtcaggg
    12421 caatgctggc aaagcccagg gcaataacaa gctgggtgat gactatccac catcgttttg
    12481 ttttaagcag gtcaaccagc gggctccaca ggggtttgat gacccacggg agataaagga
    12541 ggctggtgta cagggcgatg tcggcattgg agatgcccat ccgcttgtac atgatcaccg
    12601 atataacatt gactgccaca tagggcaagc cttcggccag atagagggtc ggtatccatg
    12661 cccatggcga acgtgacttt tcttgtgctg ccatatagtt ctataacctg ttgaatgtgt
    12721 atccttctga tgtatagtaa tcaatcagct cttccagtct gagccagagc ttgtcagttc
    12781 gttccggtac cgtgccgggg tggatcagga ttattgctcc gttcagcccc tgcggtgagc
    12841 tctccccgaa ggaggccagt ctctgaatga gctggtccga tgaacggtaa ttagccatgt
    12901 caggggtggt gtagtctgcg ttggtcccgg ttccaggagt gaagttgaca agcttcattc
    12961 ccatatcttc tgtccaccgg ctgattgcgc tgttgtacca ttcgtacggt gcgagaaacc
    13021 agcggggaat ctccttcttc agccctgcct tcctgagagc tgcaatattg cgtttcagat
    13081 catcattgaa ctgttgcttc gtaaccagca gggtatcacg gttctcccac gggatataga
    13141 gaaggtgccc gtcagaatgc ggacccacgt aatgccctcc tgctatcatc ctcctgaccg
    13201 cggaagcaaa gctcctgttc ctcaggaaat ttcctgtaag gaaaaatgac cccgtgatat
    13261 ttttgtcttc cagcacattg agtatatgct caagaccctc tccgtgctca tcggcagaaa
    13321 agaccagatg gatctttttc tcccctgctc ctctccttat tatggctcca tgggcatcat
    13381 agacgtcatc tgctgattca gccatgccgg ttctcccctc cttctcaagt gcagaaagat
    13441 aatagctgag gctggccgtc ccgtcaagag tcggctcgtt ggtgctgtaa tcaccaatat
    13501 catcatgata gacggccctg cctccctgga atgccgcata gggatcaggc ctgagaaggg
    13561 tcagccacaa gaggctctca tgaatagcct tacttatggg accgtcaacc agtccaccgg
    13621 tggttgtctc tcccaggaaa accgtcactg cagaatgggg atattggggg taatcgccgc
    13681 ctgccggcaa tccgcatatc attgatgtgc cccatgggtt gcacccgaag agccagtcac
    13741 gcaaggcagc ctccatgtag tcaaagcggc catcgccggt tgcctcacgg tagagtctgc
    13801 actgggtcag tgcagccacg acataattat tcgagcacca cacaaatggt atctttacct
    13861 ggaagagatc atccctcgtt cttgaattga tgatttccaa tcctttgcgc atgaactcaa
    13921 tgtactttcc cgaatactcc gttcctgaga gggcaaggcc tgcatggccg aggttgacaa
    13981 acggatagaa ctggtagtgt ctggcagtat ccttctctat ccagggagta cagggctcaa
    14041 gcgttcccca gtagtcggcc tgttcaaggt accagttgtt gccggtcagt ttccatagct
    14101 cccatgccgc aagctccata tcatcaacat aattgtcttc ctcatagaaa tagggagaga
    14161 cgttgcaggc tgtctgtgtg gctccggggt ctgtgcaggc aaattcaaaa gcgtcagccg
    14221 ccctgtctgc cattgtccct gcaaaaaggg agtcactccg ggcgaagagc ctggcgccca
    14281 gtgcaaatac cgaagcaaac tttccggccg tggaggagac cccctcggta cggttcttga
    14341 acgctgccag cccctgaggc ttgccggtca cgaagtaaac cggcctgtag ggaccatggc
    14401 cgtaactgac agtgtcatgg ttgggcagcc ggaaacccct gtgatcacgg tcatcagcca
    14461 cctggttgaa catcacaccg ctgtcaggat tcatcttcag aagccactcc aggccccatt
    14521 ttacctcgtc aagtatgtca ggtataccat tgctgcctgc tgctccggca gcatcccaat
    14581 tgtcggcata tatttccggt gacttcatat aggcaaacat catctggtat accgagttgg
    14641 cggtagtggc tgtgtacctc aggtgatcac tggcatcatg gtaaccgcct cggacatcta
    14701 tcggggtacc cgagagtgtc gggtgatcta ctatcacccc gtcatacctg tgacatgagt
    14761 cggcaagaaa ggggttatac ccgcaacgct gctgcctgat gtaattgagt atgaagtcag
    14821 cagttccgtc gtatgcgttt gacgcaaggc gaaaggtttc cgactttgtg cggctatata
    14881 ttatatagta tcctcctggt gttgtcagcc ctgaaaagtc aagcctggcg gcagcggcca
    14941 ttccccagac ggccccgtca cagatcacgg cagcgccacg gaaaacaggt ttgtcagtga
    15001 gcgcatcata taccgtgaat gatttttccg ctaccggttc ctgtgagata tagacagcca
    15061 ccttgaccga gcccggaagg taacccagct gattgatcct tatccatgaa tcagccctgg
    15121 caatacccgc ggccagtacc gcaagcagga gcatgagaac aactcttttc atctcagtag
    15181 agttttttta tttctgaacc tctgaagtaa tgagagagaa tctgcctgta ggtatagccg
    15241 gtggctccca tgaccgcagc tcctatctgg cacagaccca ccccatgacc ccatccggca
    15301 cctctcagca caaagagggt atcaccattt tcttcagttt tttccacaat aaaggcggag
    15361 ctgtaaagat gtgttttgga aagcaccttt ctgatctcca gctccttgcc aaaaaccata
    15421 cttttccggt caccggtaat cctgagccgt atgatacggc ctgaggttcc tctttcaaga
    15481 ggttcgagcg atgtaattcg cccgaagtcg attcctgttc tctcccttac caggtcactg
    15541 agttcatcct gacgccaggt taccttccac cggaaaaagt cattggtctc ctggtcgtaa
    15601 ttgttgagaa cctgccggag aatattcctg tcagtggtat tgcagaaggc ttccggattt
    15661 ccggttatcc acccggttgc atttttttcg tcagtgaggt caatgtccgt ggtccgggct
    15721 ccggcgccgg catcgattac cttccggaga taaggatgat tcaccggttc ccatacattt
    15781 tcgaaaagtt ctgttgcacc tccgcagcac tttgaataac gggcatcgca tataaaatca
    15841 ccgtacatca gcacctcccc gccggttttt ctcacagcct cttcaacagc gttggtcgat
    15901 gcccttgtga ttccctggta cctctggcaa tgatcatcag cgcatacgtc aaaatccctg
    15961 tggtcttccc tgtcatacca cctggtgatt tcttcgtccg tgacaaagct ggtgcgatat
    16021 ttttcgccgg tgtcgatcag ttgcagggtc ttatccatct gggcgagcag ccagctcctg
    16081 gatattacgg catgagcctt cagaagctct gatgacgagg tggcgctcat ctcggatgat
    16141 atgacactca caaggtactc ctcaacggga agaatattca ctgctgtcac ctctccgccc
    16201 tgttttatga atttcaggtc acccctgaag gtctggtctt cattcctttg ccagtgaaaa
    16261 ttgacgccta tggttacggc atgaagggtg aagctgcaac tcctgaagtc tgacggcgtg
    16321 aaggttattt catcgccggc cctaacctgc ccggcttcat tcatcaggag cagcccttca
    16381 gcatctgcaa cggcagtata gactcccgga agcatcttcc ggctgtcgcc ggcaaagaaa
    16441 tcgccgttaa gggtaaatgt cagtccgctg cctgtgacaa ttcccacact gatctctggt
    16501 tctctttttc cagtcatagt aattcctgcg tcaggatttt caattcatct tcccccaggc
    16561 atacctcatt gagaatgttt gtcacatcct cagccgatat tttgtcgaag tcctcacccc
    16621 tgggggtgat gaagactccc cccagatcaa ccgatcccgg gcttagcagt atcctgttgt
    16681 cgccttcggc gaaatagcac tgtggcctgt gcactctcct cgggataatg tgaaccgtcc
    16741 acctctcttc atcatggtaa gccagaatgt taagcatcgg ttcgggacgg tcaggctcca
    16801 ggagggccag cctgtcatgg aatctggaga aagttgcggt cagggcttca gcaccactgc
    16861 cacggagagt cagaatgccc cttccgtaac tgctccagag caatagatcc gtgccatcct
    16921 tttcatcaac caccctgcat agtgagggat tttccacatc cttctcaatt ggcaggaagc
    16981 ccctgttgcc ggcctgaaaa tggagatgat ccggagctga tgcaccgcac tgcggaccgt
    17041 tgtaaaagat aacataatca gggagcgcct gcgccaggct gagcattgta tcaaagtttc
    17101 cggatatccg ttgcggagtg tgacactgtg aaacgatagt cagatgacga cgaaaaatcg
    17161 ggaaaggatt aatcagtatt atgtagtcat ctccgaaggg aatgcccctc tgttcaccgg
    17221 gccggttctt ctcacagagg aaacatggcc tcgccccgat ggactttgca tcgacttttg
    17281 ccgccgagga ggtgatacgg cccggattga actgtacgga aatatcatag ccgggaaaag
    17341 ataaagtgcg taccatcact ccgtcaagcc gggaataatt gtcacgggcc agggcccatt
    17401 ccctgagctg cccctcaaac agatccctta ccttctgcga ataatcagtc attcatatcc
    17461 tgttttcaac tgccggccag catctttttc cgtgccagca actcaattgt ccgtatcctg
    17521 tccttgtaaa gattatgggc atttgccttt gccacatcaa gcaccgcatc ggagttctct
    17581 tcccacctgc ggcacagata gagcggttca taaatgcgcc ctatccggta atggcggctg
    17641 atggcgaggc ccactgcata atcttccccg tagcttacgt tgggaatcct gatttttctt
    17701 agaacgggcg tataaaaggc cctcggtgca ccgaggccgt ttatcctcag tgcattgtta
    17761 cggccgttat caggagtcca ctccctgtgg tctatcagac ccggcggaat ctcatccaga
    17821 ttaaaattga ccattctgta cgatcccaca accattgcac actgctgctc atggaaggcg
    17881 tccacaatac gggtgaccac gctgtcatca atgtacagat catcgctgtc aagctgaatt
    17941 gcaaacctgc cgcagcggtc gctgaaaacc gcctcgttcc agcatccccc gataccaagg
    18001 tccttcctgc cgggaatgat gtgcaccaga cggctgtcac cggcaaatga cctgatgatc
    18061 tcagtggtgc cgtcagtgga atagttgtca accactatga gattgaagcg gtactttgtc
    18121 ttctggccaa gcactgacct gatggcatct gcaatggttt ttaccctgtt cctgacggga
    18181 ataaccactg atgcatcata ctcaaaatca tcatcaaagc tgacatcacg gaaccgggga
    18241 ggcagccatc cgccaatgca cctgagatga tcagtgcagg ctttctccat ctccacctgt
    18301 acagccctgt tgcgggggtc aacataatca aactgcttct ctcccgtctt acgggtgtct
    18361 gtctcaacct ccgtataaag gagttccgga atatgaatca ggggatattt ctgtgatatt
    18421 ttcagtctca ggtcgtaaag accggcaaaa gcatactccc tgtccatgcg ggcgcaggca
    18481 tccctgaacg ccttcccgct gtacagtatc actgacccga agttgaaatc gtcacgaagc
    18541 gccccctcct ggtaatcaat taccggatga ggagctattg catcaccttt cttctcataa
    18601 taatcagaat agaccattcc tgcaccggtg tcaccgcata cctgcttcat ccttgccagt
    18661 gcatggtttc ccatgtcaag ggggaatccc ttgctgtaag tgagcacata gtcactctca
    18721 acagcagctg ccgccatctt cctgagggca tctgtcgcgg cgaatccttc cgaagtgatc
    18781 agcccggcct ctgacccgtc agggagtccc ctgctccccg gaggaagtac gacaaacact
    18841 ctgctgacaa gtcctgaaga tctcagtgaa gcgattgaag ctgtcactga tccggtatcg
    18901 gaaaagggca ggaaacaaat taaggattta tccatggtat atctcttatt tcaataaaaa
    18961 gtcaagtatc tgtttcataa gctcatcacg ctccttccgg ctgatgatgg tctcaaaggg
    19021 gaatcccatg acgaagctcc ttacatcacc cgtgtacatt acagctgcag aagtgttgtt
    19081 ctcgaggtac ctgaatgctg ttaccgactg cctgccggca ggttcaattg catcaggcga
    19141 ttcggcagca taaatcttat cggtaatgcc ggtattgaat tcaaaccggc cggtgaatgc
    19201 aggtgctgcc ttatcggtgg cagcaacgct gcctgtcctt accgcatgac cggtccgggg
    19261 catgaaatgc agggttttct tcaccagcga tgcaactgaa gtgtcattgg tcattaccag
    19321 gtcagtgccg ggatatgaac ctgacatgaa tacggggagt gatgctttct gcatcctctc
    19381 gagggtccgg aggaattcag gggtgtagat acggaaatct ttcctgtcag gccagtatgc
    19441 cgacgtggtt gtcttttcct ccccgaagag gagatcaacc acacaccatc gggagagatc
    19501 aaaatcatta cccgtgaaga cctcatcact gacggagaag aatgatttcc cggcagctat
    19561 cactgactca ccgtgaacac gggtgaaatc aaaggtgttt cccggtataa tccttccctc
    19621 atcatttgag tatgaagcgc cccagccggc attgtcatca tcggtccatg gcgaggtccg
    19681 ctcaaaatca tactggtcgc ccgtgctgat gaaattgtac cggtcagcca caccacgatc
    19741 atcccaccat gccaccccgg ccatgccgtc acggtcaaac catgcggggc cggaaacgcg
    19801 atcaaatcca tttactatca gaacaatatc atcagaggca ggattgactg caacagacaa
    19861 ctcctctgac gggaagcttt cgcctccgtc atttactgct gttaccctga agctgtacac
    19921 ggtgttgtaa tcaggcagct cagtgacaaa ggtagttccg gaaaccgggg tgccgttgtc
    19981 aaatccgtca tcaccactgc gcatgtagac cctgtatgat accgggtctg ctgttgactc
    20041 cagcggatcg gttaccggct gccaccggat gctgacccgc ctgccctcaa ccggctcaat
    20101 tgccagatga gaaacaggca gtggctgaac gacatactcc ctcccgccgg catcagcaag
    20161 gtacctgaga atgcccttgt agattgcccg gcttacatga aagcggaacc cgggatcaaa
    20221 gccgtaacgt tgatcagcca tgttctggtg cgacaaaagc tcaagaagca tggcaggcac
    20281 atcaggcttc ctggcctcat aatatgacct gtcccacatg gcacggcggg tccagtcagg
    20341 attgaaaagc gccctgatgt cttcaacgat ctgggtctga acaatatcag taaaatctct
    20401 gctggccagc ctgctggtac cgtcagggaa caggcctcca ttggttattg ttgagtatat
    20461 tcccagggtt cctattatcg aatcatccgg agtaacaccc gcatcagtat gaaaggcaaa
    20521 cgaaaggtca acaggtatgc ccaggcctcc cgaaacggtt gtatcaggcc tgcggagcag
    20581 gtaattgacc cactcggccc ttgacatgta atcatcatta taatcgttcc ttcccttgtt
    20641 gggggtgtaa accagcgaat caggcatacc tgcatactgg agccagtacc ttgctccctc
    20701 gaggaacctg ggtttgccac tcagtttcca gctgtactca cggggagccg atggcagtga
    20761 gtctgccgtt gcctgctgac tgccggcatt gagtgaccac tggttcgata tcattgattc
    20821 tgccggcctg cgggcaacat tccccatgcc gccgccaaaa cgaactgcat ccagcagcgc
    20881 tgctgagcca tccatccccg aaatggtcac cgagcctttc aggggatcag cacctgtacc
    20941 gaagtggaat gttccgagcc atagccacgt gcccccacct atcgactggt caacaatgaa
    21001 ttcactgaca cctccggtat gagtgaccct gaccgttgcc tttcccttat agtcaggcac
    21061 ccgtggatat gagactgaca cgccgtacca tccacgttca gggatttcag gaacatagag
    21121 tgccgagccg ctgctgattt tcagtgaagt tcccgccatg aacggattat caccattaaa
    21181 gagggtatcc ttcagaagaa acccccttcc tgcattttca ggttctcccg gaatttgtaa
    21241 tacaaacccg gaagcccctg tggaaatatc attgtctact ataacctcat tcacctgaat
    21301 atccctttca cgcggcagaa gcacaaccgc acccgcattt tccagcatgg gagtcaggta
    21361 aggcagcaca taggccataa ccgacatatc ttcaacactg ccgaacagct ttgccctttg
    21421 ccactcccac ctgtcaaggg gcatatcaaa ataatacccg tggctgtgcc acagagctat
    21481 gtgctttccc cccagaccct tttccggcga gacggcattc accctccgga taagcaccgg
    21541 aggatcttct gcaccaggta taatcctgtt gtcatccacc ggcatggtgt tcctgaagta
    21601 attagggacc aggctctcca ggctaaagcc gttcgaaatc atattgatcc tgtaaccggt
    21661 gaattttctc ccaagtgcag accttacaga gttttcaagg ctggtgacgg tctcttccct
    21721 gaccgggatg taggaggctg ccacggggaa ccagatattg atttccctat tctcttcatc
    21781 aaccttcaat gaatcaatga taaattgttt cggtatggct ccttcgaaaa gagggctgac
    21841 ccatttttcc atcttcgaaa cggcaactct ttcggcccgg ctggctttct gcccggcaga
    21901 ttgctgaccc ggaattagaa tcatcatgat aaaagtcagg aaatgaagga gatatgctat
    21961 tcgcattttc gaatatattt tgctgaatat actaataatg attcaaaagt agaaagtttt
    22021 ttaaaatatg tcatacatct gacaaaaact gata
//
LOCUS       DAOPBK010000015        21932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2524_length_21932_cov_4.864104, whole genome shotgun sequence.
ACCESSION   DAOPBK010000015 DAOPBK010000000
VERSION     DAOPBK010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21932
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2524_length_21932_cov_4.864104"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..486)
                     /locus_tag="PLA17_01735"
     CDS             complement(100..486)
                     /locus_tag="PLA17_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK84163.1"
                     /translation="MKDPSSSKRMAILIVEDDPYSQLYFAKLLSDMYDTAVADCAADA
                     WDLLHDRKFDLVLMDISLKGDEDGLSLTRRLRAEEDFKKLPIIAVTAYAFPDDRKRAM
                     KAGCTDYLPKPVSSNDLYRKITELTR"
     gene            complement(483..1589)
                     /locus_tag="PLA17_01740"
     CDS             complement(483..1589)
                     /locus_tag="PLA17_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPK84164.1"
                     /translation="MGPSEVFFTDMHSTSSLNLLKKLEKLMMAAGFEKIDFNRKMTAI
                     KVHFGEPGNLAYLRPNFAAQVVSMIRGKGGKPYLTDCNTLYSGERRNAPDHLDAAFSN
                     GYNILSAGCHVIIGDGVKGTDYREIEVNLDYTRTAMIGTAIADADIIITLNHFKGHEQ
                     TGFGGALKNLGMGCASIGGKLFLHSGNQPVIDLKNCTGCNMCVSSCAHDAVHMSEDGK
                     AQIDNERCTGCGQCVAVCQFDAARVRWSADGDILSKKIAEYACAVVKGKPALHVNFIM
                     DVSPDCDCWGHNDMPLVEDIGIAASTDPLALDKACADMVMEAPAARHLRYKDNDLRGE
                     DKFRIAHGNTDWNIGLLHGEKIKLGSLSYKLIKI"
     gene            complement(1610..2590)
                     /gene="pfkA"
                     /locus_tag="PLA17_01745"
     CDS             complement(1610..2590)
                     /gene="pfkA"
                     /locus_tag="PLA17_01745"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPK84165.1"
                     /translation="MAKINKIGVLTSGGDAPGMNAAIRAVTRTAIYNGLEVVGIRHGY
                     MGMINANFKQLYAHSVSDTIQRGGTILKTARCDPFRTPEGRKIAYENLRAAGIDGVVV
                     IGGDGSFKGAGMFNGEYNIPFVGVPCTIDNDIYGTDYTIGYDTALNTVVEAVDKIRDT
                     ASALNRMFFVEVMGAEAGHIALYSGIACGAEAIIMPEIKGEMRDITKMIETGNKRKKS
                     SNIIIVAEGDEEGGAIALAEKLKPQLKDYEVRVAILGHLQRGGSPSALDRVNASRLGN
                     AAVEALLDGQQSVMVGLQNDEIVLIPFRKAVKLHKGLNQHLVDIIDILNV"
     gene            complement(2659..3534)
                     /locus_tag="PLA17_01750"
     CDS             complement(2659..3534)
                     /locus_tag="PLA17_01750"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPK84166.1"
                     /translation="MKVDIEESSGFCFGVKNAVTIAEDVLRDGETVYCLGEIVHNDIE
                     VARLTSLGLKAITYEEFATLRDNKVLIRAHGEPSSTYEKARENNITIIDATCPIVRSM
                     QEKIRKVAELSRDEKGQIVIYGKEGHAEVIGLMSAAGPGGILVTGKGDLRRIDYTRPV
                     YLFSQTTKSKVQYDVVAEIIRLNMEKVSPDHSSSNLKVHNTICRQVSEREPRLREFAS
                     KHDVIVFVSGRNSSNGRMLFEVCLKVNPRSYFISSPDELKRKWFNDSASAGICGATST
                     PGWLIQEVADIVAAI"
     gene            complement(3524..4228)
                     /gene="cmk"
                     /locus_tag="PLA17_01755"
     CDS             complement(3524..4228)
                     /gene="cmk"
                     /locus_tag="PLA17_01755"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPK84167.1"
                     /translation="MTRKKRKIIITVDGYSSCGKSTFAKAIARELGYIYIDSGAMYRA
                     VTLFALRRGLAGNGIADEAGILQALPEITITFKPDSETGFSATYLNGEKVEREIRDRE
                     VSDNVSPVSTIAGVRARMVELQRSMGAGGGVVMDGRDIGTVVFPDARLKIFMTARPEV
                     RAQRRYLELVTKGLPADFDAVMKNISERDRIDSSREVSPLRQADDAVVLDNSDMTPDE
                     QMVWFRELYRKRINES"
     gene            complement(4261..5346)
                     /gene="porQ"
                     /locus_tag="PLA17_01760"
     CDS             complement(4261..5346)
                     /gene="porQ"
                     /locus_tag="PLA17_01760"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HPK84168.1"
                     /translation="MILRMRHRAPNLLSGIAVLQRWAVVLIFLTALPGPALKGQTGGD
                     NTYEFLNLSHSAFANATGGISVASWRSDLTLPYFNPAMLNAAMDNDISLSFSSWFAGI
                     RYGYASWAHSTATAGNFAGGISFASYGTFTEADEAGNITGSFTAAEYGFNIIWSWQID
                     SSFSVGVNLKPVISNLERYFSAGLCADIGATYRNERLLLDAGLVIRNAGLQLIGYTGD
                     GGEPLPFEIIAGATKRLAHAPFSFSLTLRHLEKPDMTFDYNPEDDAGQGGFGENILRH
                     IVLGVEILPSDAFWIGAGLDYQRRAEMRTGQRTGMAGFTAGFGIRTKAFELAFAHGAF
                     HPAGGSNHITVTLKPDIIFKKTEHAEP"
     gene            complement(5407..6321)
                     /locus_tag="PLA17_01765"
     CDS             complement(5407..6321)
                     /locus_tag="PLA17_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPK84169.1"
                     /translation="MKGRPYAALVLSMIFWALSFVWFKVANVDYNPITIVFIRLCIAI
                     LFLTGFIWITGRFTAIQKRDRKYFFLLALFEPFLYFLGESFGLTYVSSTVGSVIISTI
                     PVFAVIFAWVIHRERLKLINYLGVILSFIGVLIFITNKSGNIVMNPKGLALMFLAVVS
                     AVGYNMVLRRLAYDYDPVIIVNTQNIIGAILFLPLFLVLDLKDLLATGIVTKSFGSII
                     FLAIFASCGAFVLYAYSVRFVGIARANIFSNLIPVFTAIFAFFIVGDRLTVRNGIGMA
                     IVIAGLFLSQAWNNRKAGVDAGFTGKGD"
     gene            6574..7437
                     /locus_tag="PLA17_01770"
     CDS             6574..7437
                     /locus_tag="PLA17_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPK84170.1"
                     /translation="MNGIKIVVLAKQVPDTRNVGPDAMKADGTVNRSVLPAIFNPEDL
                     NALEQALRIKERYEGVTVTLLTMGPVRAAEIIREGLYRGADDGVLLTDRAFAGSDTLA
                     TSYALALAVRRLAPDLVIGGRQAIDGDTAQVGPQVAEKLGWPQVTYAEEMHFEGDKLI
                     IRRRLERGVETVSAPLPLVVTVNGTAPACRYRNARLVMKYKYARTDTELQTESSDYIA
                     AGRSSIRLTEWGVDAIGADREQLGLSGSPTKVKEIENIILQAKESKRLTSSDADVESL
                     VRELLEDHTIG"
     gene            7462..8481
                     /locus_tag="PLA17_01775"
     CDS             7462..8481
                     /locus_tag="PLA17_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPK84171.1"
                     /translation="MNNLIVFCELDEGRPADVSLELLTRGRSLANTLGCRLEAVVLGN
                     GLEGIENELFPYGVDVVHVADDPVLEHYRTMPYTSVITGLFRQEKPQIALMGATSTGR
                     DLGPRVSSLLHSGLTADCTSLEIGDHYVSKTDTTYKNLLYQIRPAFGGNIIATIVNPD
                     HRPQMATVREGVMKREIADKNYRGELRKIDVASFIRPEDLAVEIIERHMEARKVDIKS
                     ARIIVSGGYGMGSKENFAMLYELASVLGGEVGASRAAVDAGFAGHERQVGQTGVTVRP
                     KLYIACGISGQIQHTAGMNQSGKIIAINNDPVAPINTIADYVIHGDVATVIPKLIKYY
                     KANAR"
     gene            8517..10223
                     /locus_tag="PLA17_01780"
     CDS             8517..10223
                     /locus_tag="PLA17_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPK84172.1"
                     /translation="MANFYTDNKDIRFHLRHPAMEKIVRLKEMDYRDKEKYDYAPHDF
                     EDAMDNYDKVLEIIGAICAEVIAPNAEDVDLEGPTVTGGHVTYARGTRENHETLTKAG
                     VIGMNFPRQYDGLNFPMVPYVMAAELVSRADAGFANIWGLQDCGETILEFASDEIKQE
                     FLPRFNKGATASMDLTEPDAGSDLQAVQLKAHYDEERGVWLLNGVKRFITNGDAEISL
                     VLARSEEGTSDGRGLSLFIYDRADGGVIVRRIENKMGIKGSPTCELAFRNVPVKLIGE
                     RRLGLIKYVMSLMNGARLGVGAQSVGIAQAAYEEARKYASERKQFGKTIDQFPAVYEI
                     LTLMKVRLAAMRSLLYETARFVDTYKAWSFIAAERQLTKEERDDLKINTRLADIYTPM
                     VKLVSSEWCNSIAYDAVQIHGGAGFIKDFPVERLYRDARITSIYEGTSQLQVVAAIRG
                     VTTGGYLNQIREYEKRELRPELDHLKKELVSLTDDYEKRVAQVVSAGNNELTDFHARR
                     LVEMAANIVMGYLLLDDAQRDACRTSLAELFIPLAVSENRVRSEYIDSFEEKLLGLYK
                     IG"
     gene            10396..13473
                     /gene="ccsB"
                     /locus_tag="PLA17_01785"
     CDS             10396..13473
                     /gene="ccsB"
                     /locus_tag="PLA17_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPK84173.1"
                     /translation="MKALRFLISPVMMAVLFIALAASLAAATFIENDFGQEAARAAVY
                     NTRWFELLFLLLTLNLAGQIYTFRLWRREKLTLLLFHAAFIVMVAGAAITRYTGYDGM
                     MHIREGDSSNVTYSSGATLIFELAGDDGALPDSHSEPLSIYGGRHGKYCKSLEADGTK
                     ATLTFERYIAGAVKTVQETPGGEPMVSLLFTSDMVSSETVVLVLGRTVMIGDMKAGFG
                     TPADITVTTDGNLFFISSSTGLRSTAMQEMKSAEHPADSLVPLMAGTVYTAGSYRIMP
                     RIMTMSGAIVPSVTAEEQSAGGALEFSLKGDGFQETIWLWDDDGEERSEWSGDAGRLT
                     ARVSYGASLRELPFSLRLDDFIVEHYPGSNSPSGFTSRVILSDPEHGTVMPYDIYMNH
                     ILKYRGYRFYQSSYDSDELGTVLSVNHDPAGMYTTYTGYGLLFLFIILSLLNRKSFFR
                     TVTPGHWASNYRKPAAALVLILLTSLSPLKSQHLVIERKAADSFGAVLAQDQKGRTKP
                     LYTISSDILRKVAGENSFEGMSPMQVFLGCSFDFYHWQNVALIKVSSAELRRELNLEG
                     EKAAFSDIVKMAKDGGYLLGPLVDKAYAKSVTERTKFDKEVIKVDERVNICYMMSRGD
                     FMHIFPLRDGTDHWGSAEEAVRHGIGQEDSLFVISLIPLWAQSVTSVGHPMAPPADYI
                     TALTDYQHRHAGYELPSECKVKAELFYYKARIFEKLFPWYAAVGLIMIIAIITMIIRG
                     RELSGAVLKVLALLVAAGFLFHTLGLGIRWYISGHSPMSNGYESMLFISWVTLLAGLI
                     FSRRSLLTLAATSVLGGLTLMVAHLSFMDPEITNLVPVLRSYWLTLHVSVITGSYGFL
                     GLGAILGIIVLVMMLFIHDGNRARISAVIDELTVINYRTLTLGLYFLTIGTFLGAIWA
                     NESWGRYWGWDPKETWSLITIIVYTLVTHSRMMPGMKETYIFNLLSLFALSSVLMTYF
                     GVNYYLSGLHSYAGGDAVPVPAFVYVAVAVLISLAVAAGYKYRRALKRQVGGN"
     gene            complement(13653..13728)
                     /locus_tag="PLA17_01790"
     tRNA            complement(13653..13728)
                     /locus_tag="PLA17_01790"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13691..13693),aa:His,seq:gtg)
     gene            13888..15234
                     /locus_tag="PLA17_01795"
     CDS             13888..15234
                     /locus_tag="PLA17_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPK84174.1"
                     /translation="MSRLKIIDNISGLQAVQLLRFLTFLIISIVFTKSHLTRAQIGNW
                     ELFLFISSLLSFFWVTGIIQSLLTLYERNRTFKTDGTRAGRKSPEIYNAFLLISFFSL
                     MVFILGIPIRFMLESSQADTDIPYPNLLLLYILVSNPVALIEYIYLLNKRPHRILQYG
                     IYTYSAQLLLVLAPVIAGYDEKSAIWGLFAITAARWVWLITLLRRYTLLRVSPEFIRE
                     HLRVGMPLILAFLISGSAQYVDGIIVSARYSDPGVFAMYRYGAKEFPLVLLLANGLSN
                     ALLPQFSTREGMREALVTLRKRSDRLIKYLFPISMVTMLVARWVYPRLFTPEFTRSAD
                     VFMIYLLLIIPRLVFPQTIVIGRRKTNVTLYTATIELAVNIPLSLLLIKPYGVVGVAL
                     ATFIVYFLEKIFLIYYVWKKMKIRPAEYIPLTRWIIYSALLILLFVLIDHRIIDIY"
     gene            15324..16811
                     /locus_tag="PLA17_01800"
     CDS             15324..16811
                     /locus_tag="PLA17_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPK84175.1"
                     /translation="MAFHNSSLEDYFGTFRRNIVGIDQNYISPYGLKKIIYCDWIASG
                     RLYGPIEEKISHIIGPFVANTHTETSETGSMMTWAYHYAQEIIKKHVNAGPDDVLIGA
                     GAGMTAVINKLQRILGLKGCLYPGKAGCLDATDRPVVFITHIEHHSNQTSWYETLADV
                     VIIEPGSDLTVDPENLRKALEKYSDRKFKIGSFSGCSNVTGAFTPYHTLARIMHEHGG
                     LCFIDFAASAPYVDINMHPDDPMEKLDAIFFSPHKFLGGPGSSGIMLFDKSLYKSSVP
                     DQPGGGTVDWTNPWGEYKYVDNIESREDGGTPAFLQMMRAALAIRLKEQMGTAKIMAR
                     EEELLAMTFSGLSAIPGLHILASNVRHRLGALSFYIDDLHYNLVVKLLSDRYGIQVRG
                     GCACAGTYGHYLLDVTYDHSRRITEMINRGDLSEKPGWVRLSIHPTMTDAELEMVIAA
                     LAEIRDNAAEWSKDYIYSRRTNEFTHRDQTGTGMERVSPWFNLQT"
     gene            16817..18025
                     /locus_tag="PLA17_01805"
     CDS             16817..18025
                     /locus_tag="PLA17_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84176.1"
                     /translation="MKITEVTTESLRKEFIDFPKKLYRGDPNWVCPLDSETEAVFSHE
                     RNVAFRHGEAVRWILSDGDGNTIGRVAAFIDEIRSKANRQPTGGLGFFEVIESRDAAF
                     LLFDTARQWLTERGMKAMDGPINFGDNDNYWGLLVEGFMQPGFGMPYNKPYYKHYFTD
                     YGFRTYFEQYSYHKDVAAVEVFPERFRKIAEWISKRPGYSYRHFDFSQRDKFAADMVE
                     IYNATWAVFKEDFTPLDPARIEDTLHQASAFIDPELIWFAYHNDKPVAFFIIFPDLNQ
                     IIRHFNGRLTLLNKIRFLWYKMTHEMTRVRAFVAGVHPSYQNAGIESAIFLELFRVFR
                     KKRYYRELELSWVGDFNPKMISIYEAIGATRAKVHLTLRYMIDDSLPFKMYKDEMEEN
                     RAARQAGQKK"
     gene            18122..18388
                     /locus_tag="PLA17_01810"
     CDS             18122..18388
                     /locus_tag="PLA17_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPK84177.1"
                     /translation="MKEGIHPNNYRLVVFQDMSNGYTFMTRSTVSSRDTIKWEDGKEY
                     PLIKLEISNTSHPFYTGKMKLVDTAGRVDKFMNRYKDHLAKKTK"
     gene            18526..20988
                     /gene="lon"
                     /locus_tag="PLA17_01815"
     CDS             18526..20988
                     /gene="lon"
                     /locus_tag="PLA17_01815"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPK84178.1"
                     /translation="MEKKVYGNWDRIGLRELIDEGREIIPILTDGDDQELEDVQIPGS
                     IPLLPLRNTVLFPGVVIPIAVGRSKSVQLVRDAYRGDKLVAAVAQLDAEVDDPGVRDL
                     NAVGTLGHVVKLLEMPDGTTTAIIQGRKRVAIDNIIQTTPYMTAQIRAMPEPRGVDDE
                     KDFEVIIGSLKDLSVKIVKLNPNISPEATFAVRNIENSTYLINYICANTEINVTDKQK
                     LLEAGSIRERGLRLLEHLVREIQLLELKNELQSKVKYEIDQQQREFLLHQQMKTIQNE
                     LGGSPIEKEVEEFRSRGAKRAWGEDVAKVFNRELDKLSRLNPAAAEYSVQVNYIQTLL
                     ELPWNEYTTDNLDLENAKSVLDEDHYGLEEVKERILEHLAVLKLKGDLKSPILCLYGP
                     PGVGKTSLGKSVARALGRKYARMSLGGLHDEAEIRGHRKTYIGAMPGRVVQNIRRAGS
                     SNPVFILDEIDKVGQDFRGDPSSALLEVLDPEQNTHFYDNFLELEYDLSKVLFIATAN
                     TLSTVSPALRDRMELIEISGYLVEEKREIAVKHLIPKQLDAHGVTPEQFMLSREVIEE
                     VIQKYTRESGVRELDKRIAKLVRFRAREIAFENKPEPEITSAMVEEILGPPKYLKDIY
                     DGNDQAGVVTGLAWTATGGEILFVETSLSRGKGNLTLTGNLGEVMKESAVIALEYLKA
                     HASLLNLPETFAEKWNIHIHVPEGAIPKDGPSAGITMAVSIASAFTQRKVKKYVAMTG
                     EITLRGKVLPVGGIKEKILAAKRAGIKVIVLSEHNRKDVEEIQEKYVKGLKFKYVSTI
                     MDVLDHVLLQQKVANPRIIEFE"
     gene            21004..21480
                     /gene="coaD"
                     /locus_tag="PLA17_01820"
     CDS             21004..21480
                     /gene="coaD"
                     /locus_tag="PLA17_01820"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPK84179.1"
                     /translation="MDKVALFPGSFDPFTVGHESLVKRCLRLFDKIVIAVGENSDKKN
                     LFSTEMRMKMICRVFEGESRIEVTRYSGLTVEFCRSCGARYIIRGLRTAADFEYERAM
                     GQMNRKMAPEIDTVFLLTSAEHTPINSTIVRDIIKNGGDVSMFVPAAINISEYLGK"
     gene            complement(21619..>21932)
                     /locus_tag="PLA17_01825"
     CDS             complement(21619..>21932)
                     /locus_tag="PLA17_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPK84180.1"
                     /translation="GHMEENETPEECAIREVTEECNIHGHIIKGTLPPSYHTYLFDNK
                     PYMKKTSWFRMSYSGEMITDPQASEGITHAQWLVPDEIARIKSNTFQSLLDLLNFTLR
                     V"
BASE COUNT         5414 a         5451 c         5765 g         5302 t
ORIGIN      
        1 taaaagagcc tgactcattc tgttcttatt aatccccggg caccccgggt gctatgatta
       61 gtttgcaaaa gcccttatcc tgcagaaact ttttgactat taacgggtca gttccgtgat
      121 cttgcgatac aggtcattgc tgctcaccgg cttgggcaga taatcagtgc aaccggcctt
      181 cattgcacgc ttacggtcat cagggaatgc ataggccgtc accgctataa taggcagttt
      241 cttaaaatcc tcctcggcac gcagcctgcg cgtcagcgag agaccatcct catccccctt
      301 cagggagata tccatcagca ccagatcaaa cttccggtca tgaagcagat cccatgcatc
      361 tgcggcgcaa tcggccacag cagtgtcata catatctgac aacagcttcg caaaataaag
      421 ctgactgtac gggtcatcct ccacgatgag aatggccatt cgctttgatg atgacgggtc
      481 cttcatatct tgatcagttt gtatgataaa gatccaagct taatcttctc cccgtgaagc
      541 agtccaatgt tccagtcggt gttgccatga gctatccgaa acttatcctc cccccggaga
      601 tcattatcct tatacctcaa atgccgtgcc gcaggcgctt ccatgaccat atcggcgcat
      661 gccttgtcaa gggcaagggg gtctgtggaa gccgcaatgc ctatgtcctc aaccagcggc
      721 atgtcattgt gtccccagca atcgcagtcg ggcgagacat ccatgataaa attgacatgt
      781 agtgcaggct ttcccttaac caccgcacag gcatactcgg ctatcttttt gctgagaata
      841 tctccatctg cgctccatcg aaccctcgca gcatcaaact ggcagacagc cacgcactgt
      901 ccgcagccgg tacatctttc gttatcaatc tgtgccttcc cgtcttcact catgtgaaca
      961 gcatcatggg cacagctgct gacgcacatg ttgcaaccgg tacagttctt caggtcaatg
     1021 accggctgat tccccgagtg caggaaaagc tttcctccga tggaagcaca tcccataccg
     1081 aggttcttca gtgcgcctcc gaaaccagtc tgctcatgcc ccttgaagtg attgagggtg
     1141 ataattatat cagcatcggc tatagccgta ccgatcatcg ctgtcctggt ataatcgagg
     1201 ttaacctcta tctcacggta atcagtcccc ttgactccgt cgccaattat cacatggcac
     1261 cctgcgctga ggatattgta gccatttgag aatgctgcat caaggtgatc gggagcattt
     1321 ctcctctccc ctgaatagag ggtgttgcag tcggtcaggt atggtttgcc acctttcccc
     1381 cttatcatgg aaaccacctg tgccgcgaag tttggcctga gatatgcaag gttgcccggc
     1441 tcaccgaaat ggacctttat tgccgtcatc ttacggttga aatcgatttt ttcgaaaccg
     1501 gctgccatca tcagcttctc cagcttttta agcaggttca gcgatgatgt gctgtgcata
     1561 tcggtgaaaa atacttcaga aggtcccatt aaaaacaaaa cgttctttat cagacattaa
     1621 gtatgtcaat aatgtcaaca aggtgctggt taaggccttt atgcagttta acggctttcc
     1681 tgaatggaat gagcactatt tcgtcattct ggagtcctac cattacgctc tgctggccgt
     1741 caaggagtgc ctctacagca gcatttccca gcctgctggc gttaacacgg tcaagcgctg
     1801 acggcgaccc tccgcgctga aggtgtccaa gaatggcaac ccgtacctcg tagtccttga
     1861 gctgaggttt gagcttctcg gccagtgcta tggcgccacc ctcttcatcg ccctcagcaa
     1921 caatgatgat attgcttgat ttttttcgct tgttacccgt ctctatcatc ttggtgatgt
     1981 cacgcatctc accctttatc tcaggcatga tgatagcttc agcgccgcag gcgattccgc
     2041 tgtaaagggc aatgtggcct gcctcggcgc ccatcacctc cacaaaaaac atcctgttga
     2101 gggcgctggc ggtatcacgg atcttgtcaa cagcctccac cacggtgttg agcgcggtgt
     2161 catagcctat cgtgtagtca gtgccatata tgtcattgtc tatcgtgcag ggcacaccca
     2221 cgaacgggat gttatactct ccattgaaca tgcccgctcc cttgaatgag ccgtcacctc
     2281 ctataacaac aacgccatcg atgcctgctg ccctgaggtt ctcataagca atcttcctcc
     2341 cctcgggagt acggaaggga tcgcatctgg ctgtcttgag aattgtgccg cctcgctgta
     2401 tggtgtctga aaccgagtgg gcgtagagct gtttgaagtt ggcattgatc atgcccatgt
     2461 aaccgtgcct gatgccaacc acctcaagcc cgttataaat agctgtgcgc gttacggcgc
     2521 ggatagctgc attcattccg ggagcatctc cgcctgatgt gagaacacct atcttattaa
     2581 tttttgccat ttcagaaatt atttgttgat gctaaaatac ttaattatca tgttccggca
     2641 attttgaaaa taacaatatc atatcgcagc gacaatatct gcaacctcct gtatgagcca
     2701 tccgggggtg gatgttgcgc cgcatatccc tgcactggct gaatcattga accatttgcg
     2761 cttcagctca tcgggcgagg agataaagta ggagcggggg ttcaccttaa ggcacacttc
     2821 gaaaagcatg cggccgttag agctgttcct gccgctgaca aaaactatca catcatgctt
     2881 cgaagcaaat tccctcagac gtggttcacg ttctgacacc tgcctgcaga tggtgttgtg
     2941 caccttcagg ttggacgatg aatgatcagg cgagaccttt tccatattga gcctgataat
     3001 ctcagccaca acatcgtact gcaccttgct cttggtggtc tgcgagaaga ggtataccgg
     3061 cctggtgtag tctatcctgc gaaggtctcc cttgccggtc accagtatgc ccccgggccc
     3121 cgcggcactc atcagcccta tgacctcggc atgaccctcc ttgccgtaga tgactatctg
     3181 tcccttctcg tcacgcgaca gttcggccac ctttcgtatt ttctcctgca ttgacctgac
     3241 aatagggcag gtggcgtcaa tgatcgtgat gttgttctcc cttgcctttt catatgtgga
     3301 ggagggctca ccgtgcgccc ttatcagcac cttattgtcg cgcagggttg caaactcctc
     3361 ataggtgatt gccttcagtc cgaggctggt cagcctggcc acctcgatgt cattgtgtac
     3421 tatctcaccg aggcagtaga cggtttctcc gtcgcgcagg acatcctcag cgattgtaac
     3481 ggcattttta actccgaaac agaatcccga actctcttct atatcaactt tcatttatcc
     3541 tttttctgta aagctctctg aaccagacca tctgttcatc gggtgtcatg tcgctgttgt
     3601 caagaacaac tgcatcatca gcctgccgga gcgggctcac ctccctcgat gagtctatcc
     3661 tgtcacgttc gcttatgttt ttcatcaccg catcaaagtc agccggcagt cccttggtca
     3721 caagctctag gtaccgtctc tgtgctctca cctcgggcct ggctgtcatg aagatcttta
     3781 gccgggcgtc cgggaagacc accgttccaa tgtcacggcc gtccatcacc acacccccgc
     3841 cggcgcccat tgaccgttgc agctccacca tcctggctct cacgccggca atggtgctga
     3901 cggggctgac gttatccgag acctcgcggt cacgtatctc cctctccacc ttctcgccat
     3961 tgagataagt ggctgagaat cctgtctcgc tgtcgggttt gaaggtgatg gtgatctccg
     4021 gaagagcctg cagtatcccg gcctcgtcgg caatgccgtt tcctgccagt ccgcgcctca
     4081 gcgcgaagag agtgacagcc ctgtacattg ctcccgagtc gatatagata tatcccagtt
     4141 cccttgctat cgctttcgcg aaggtgcttt tgccacacga cgagtatccg tcaaccgtaa
     4201 tgattatttt tctttttttt cttgtcattg tctctttcag gttaaaacga acagatgcag
     4261 ttaaggttca gcatgttctg tttttttaaa tataatatca ggtttcagtg taacggtaat
     4321 gtggtttgat ccgcctgccg gatgaaatgc gccatgggcg aaagccagtt caaaggcctt
     4381 tgtgcgtatt ccgaaccctg ccgtaaagcc cgccataccg gtccgctgcc cggtccgcat
     4441 ctcagccctg cgctggtagt caaggccggc gcctatccag aatgcgtctg acggaagaat
     4501 ctcgacaccc aggacaatgt gcctgagaat gttctctccg aaaccgccct gaccggcatc
     4561 atcttcggga ttataatcaa aagtcatgtc aggcttctcc agatgcctga gtgtgagcga
     4621 gaagctgaag ggggcatggg cgagcctctt ggtggctccg gcaataatct caaacggaag
     4681 cggctcacct ccgtcaccgg tataaccgat aagctgcagt ccggcgttgc ggattaccag
     4741 cccggcatcg agcagcagcc gctcattgcg gtatgttgcc ccgatatcgg cacagaggcc
     4801 tgcagaaaaa tagcgttcga ggtttgagat cacaggcttg aggttcacac ccacggagaa
     4861 ggaggagtcg atctgccatg accagatgat gttgaaccca tattctgcag cggtgaagct
     4921 gccggtgata ttacctgcct cgtcggcctc ggtgaaggtg ccgtagcttg caaagcttat
     4981 ccccccggcg aagttgcccg cggtggctgt gctatgcgcc caggaggcat agccatacct
     5041 gattcctgca aaccatgatg agaagctcag cgagatatca ttgtccatcg ccgcattgag
     5101 catagccggg ttgaagtagg gcagggtcag gtcgcttcgc caggaggcga cggatattcc
     5161 gccggtggca ttggcaaatg ccgaatggct gaggttaaga aactcgtagg tattatcacc
     5221 gccggtctgg cctttaagcg ccggaccggg cagagccgtg aggaatatga ggacgacagc
     5281 ccaccgttgc agaacagcaa tgccggaaag cagattggga gccctgtgcc tcatcctcag
     5341 aatcattaag gacaaagata ataatcccgg caaaatggaa tgatcaaagc tggtatttca
     5401 ggattgtcag tcacccttcc ctgtaaagcc ggcatcaacc cctgccttcc tgttattcca
     5461 ggcctgggaa aggaagaggc cggcaatcac aattgccatc cctatcccgt ttctcaccgt
     5521 cagcctgtcg ccaacgatga agaaggcaaa gattgcagtg aacaccggaa taagattgct
     5581 gaatatgttt gcacgggcaa tcccgacaaa cctgactgaa taggcataaa gcacgaatgc
     5641 tccgcacgag gcgaagatgg ccaggaaaat tatcgagcca aagctcttcg tcacgatgcc
     5701 tgtggcaagc aggtccttca gatccagcac aaggaagagt ggaaggaata gaattgcacc
     5761 aataatattt tgagtgttga ctattatgac cggatcataa tcataagcca gacggcgaag
     5821 aaccatgttg taaccgacgg ccgagaccac ggcgaggaac atcagcgcca gtcctttcgg
     5881 gttcatcacg atattaccgg atttgttggt gatgaatatt aaaactccta tgaatgagag
     5941 aataactccc agatagttaa tcagtttcag ccgctcacga tggattaccc atgcgaagat
     6001 gactgcaaag accggtatgg ttgaaattat taccgaaccc accgttgacg agacatatgt
     6061 cagtccgaag ctttcaccca ggaagtaaag gaaaggctca aacagcgcaa gtaggaagaa
     6121 atacttccgg tctctcttct gtatggctgt gaaccgtccg gtgatccata taaaacccgt
     6181 gagaaagagt attgcaatgc acagcctgat aaagacgatg gttatggggt tataatcaac
     6241 gttggcaacc ttgaaccata caaatgagag tgcccagaaa atcattgaaa gaactagggc
     6301 tgcgtatggt ctgcctttca tgggagataa taaatgagtg attttgtcgg cggcgaaatt
     6361 aactgatttt tcaatacccg tgacagagcg gggaattaag aagctttagt tttcgaacgt
     6421 acttgatata tgttcggcaa aagcctcata ataatgtaat atacagttca atatatatca
     6481 atcatctttc gaccggtata aataaaaatc atttttattc gtttggtgat atggttttat
     6541 tattatattt gccacttcaa aagcgataat gagatgaacg gaatcaagat cgttgtactt
     6601 gccaagcagg ttcctgacac gcgaaatgtt ggtcctgatg ccatgaaagc tgacgggacg
     6661 gtgaacagga gtgttctgcc tgccatattt aatcccgagg atctgaatgc cctggagcag
     6721 gctttgagaa taaaggagcg ttatgaaggt gtaacggtaa cgctgcttac gatgggtccg
     6781 gtcagggctg cggagataat acgggaggga ctgtaccgtg gagcagatga tggcgtactg
     6841 cttactgaca gggcttttgc cggttcagac acgctggcca cgtcgtatgc tcttgcgctg
     6901 gcagtgcgca gactggcgcc tgacctggtc ataggaggac ggcaggccat tgacggtgac
     6961 accgcccagg tggggccgca ggtggcagag aaactgggat ggccgcaggt gacctatgca
     7021 gaagagatgc atttcgaggg cgacaaactg ataattcgcc gccgtcttga gaggggagtg
     7081 gagacagtat cagctcctct tccgctggta gttacagtga acggaacagc gccggcatgc
     7141 cgttatcgca atgccaggct ggtgatgaag tacaagtatg cacgcactga taccgagctt
     7201 cagactgaaa gctctgacta cattgctgca ggcaggtcat ccatcaggtt aactgaatgg
     7261 ggcgttgatg ccatcggggc tgaccgtgag cagctcggtc tctccgggtc acccacaaag
     7321 gtgaaggaaa ttgagaacat aatcctccag gcaaaggagt cgaagcgcct tacctcgtca
     7381 gacgcagatg ttgagtcact ggtcagggag ttgcttgagg atcatacaat tggttgataa
     7441 acggataaaa ctatagttgc agtgaacaat ctaatagtct tttgtgagct tgatgaaggc
     7501 aggcctgccg acgtcagcct tgagttgctc accaggggca ggtcactggc caacaccctt
     7561 ggctgccgcc tcgaagcggt ggtgcttggc aacgggctgg aaggcatcga aaacgaactt
     7621 ttcccctatg gtgtcgatgt ggtacatgtg gctgatgacc cggtgctgga gcactaccgt
     7681 acaatgcctt acacctcggt gatcaccggt cttttcaggc aggagaaacc acagattgca
     7741 ctgatgggag ccacatccac aggccgtgac cttggcccaa gagtctcgtc actcctgcac
     7801 agcgggctga cagccgactg caccagcctt gagataggcg accattatgt cagcaagacc
     7861 gataccacgt ataaaaacct gctctatcag atcaggcctg cttttggcgg taacattatt
     7921 gccaccatcg tcaatcctga ccaccggcca cagatggcca ccgtacgtga gggggttatg
     7981 aagcgcgaga tcgcagacaa gaattacagg ggagagctca ggaagataga tgttgcctcc
     8041 ttcatcagac ccgaagacct tgccgtggag attattgaaa ggcacatgga ggcaaggaag
     8101 gtggatatca agtcggcccg gataattgtt tccggcggtt acgggatggg gtcgaaagag
     8161 aatttcgcca tgctttacga gcttgcttcc gtgcttggtg gcgaggtggg tgcttcaagg
     8221 gctgccgtag atgcagggtt tgccgggcat gagcgtcagg taggacagac cggggtgaca
     8281 gtgaggccaa agctctatat agcatgcggc atctcaggtc agattcagca taccgccggg
     8341 atgaaccaga gcggaaagat catagccatc aacaatgacc ctgtggcgcc catcaacact
     8401 attgccgact atgtcataca cggtgatgtg gccacggtca ttccgaagct tatcaaatac
     8461 tacaaggcta acgccaggta aaatcaacag cagtctgaac gcaaaaagaa acaccaatgg
     8521 caaatttcta tactgacaac aaggatatca ggttccacct tagacacccg gcgatggaga
     8581 agatcgtcag gctgaaggag atggattaca gggataagga gaaatatgac tatgccccgc
     8641 atgacttcga ggatgccatg gacaattatg acaaggtgct ggagatcatt ggcgccatct
     8701 gtgccgaggt cattgcacca aatgcggagg atgttgatct tgagggcccc acggtgactg
     8761 gcggtcatgt tacctatgcc aggggcacac gtgaaaacca tgagacgctc accaaagcag
     8821 gggtgatagg gatgaatttt ccgaggcagt atgacggact gaatttcccg atggtaccat
     8881 atgtcatggc tgctgagctg gtttcaaggg ctgatgcagg atttgcaaac atctgggggc
     8941 tgcaggactg cggtgagact atcctcgagt ttgcaagtga cgagataaag caggagtttc
     9001 ttcccaggtt caataaaggt gccacagcct ccatggacct taccgagccg gatgccgggt
     9061 ctgacctgca ggcagtgcag ctcaaggctc actatgacga ggaaagaggt gtatggctgc
     9121 ttaacggggt gaagcgtttc atcaccaacg gcgatgccga aatctccctg gtccttgccc
     9181 gctccgaaga ggggacctct gatggcaggg gactgtcact ctttatttat gacagggctg
     9241 atgggggcgt catcgtcaga cgtatcgaga acaagatggg catcaagggc tcacccacct
     9301 gcgaactggc cttccggaat gtgccggtaa aactaatagg tgagcgccgt ctgggtctca
     9361 tcaagtatgt catgtcactg atgaacggcg cccgcctcgg tgtgggagca cagtcagtag
     9421 gaatagccca ggctgcctac gaggaggcca ggaaatatgc ctcagagagg aaacagttcg
     9481 gaaagacaat tgaccagttc ccggctgtct atgagatact gaccctgatg aaggtccgcc
     9541 ttgcggcgat gcgttcactc ctctatgaga ctgcccgctt cgttgacaca tacaaggcat
     9601 ggtcatttat tgcagcggag cgccagctaa caaaagagga acgtgacgat ctgaagatca
     9661 acaccaggct ggcagacatc tacactccga tggtgaagct ggtcagcagt gagtggtgca
     9721 acagtattgc ctatgacgcg gtacagatac atggcggagc aggcttcatt aaggattttc
     9781 ccgttgagag gctttaccgt gacgcacgga tcacctccat ttatgaaggc acatctcagt
     9841 tgcaggtcgt tgctgccatc aggggagtga caacaggagg gtacctgaat cagatacgtg
     9901 aatatgaaaa gagggagctc aggcctgagc ttgatcacct gaagaaggag cttgtctccc
     9961 tgacggatga ttatgagaag agggttgcac aggtggtttc tgccggcaac aatgaactga
    10021 cagatttcca tgcacgccgc cttgttgaga tggcagctaa cattgtcatg ggttacctgc
    10081 tgctcgatga tgcccagcgt gatgcctgcc gcacctccct ggccgaattg tttattcccc
    10141 tcgctgtatc ggaaaaccgc gtgaggtcag agtatataga ctctttcgag gaaaaactac
    10201 ttggcctcta taagataggc tgacgcacct ccttccgtta tcccggcatc gccttaatga
    10261 tgcgggaaag cattaaatag tcgcccggat gacaggctcc aatctgccat ccgggttttt
    10321 ttgatgcaca tgatacttgg catataaaac tgcggtgatt tccgaaaata gatagatttg
    10381 tataaaactg atttcatgaa agcattaagg ttcctgattt cacctgtaat gatggctgtt
    10441 ctcttcattg ctctcgcagc atcactggct gcagcaacat tcattgagaa cgatttcgga
    10501 caggaggctg ccagggcagc agtttacaac acccggtggt ttgagctgct ctttctgctg
    10561 ctgaccctga atctggccgg gcagatctac accttcaggc tgtggcgcag ggaaaagctt
    10621 acactgcttc tgtttcatgc agcatttata gttatggttg caggtgctgc aataaccagg
    10681 tatacgggtt atgacggcat gatgcatatc agggagggag actcctccaa tgtcacatac
    10741 tcctctggcg ccaccctcat attcgagctt gcaggggatg atggcgccct gccagacagt
    10801 cactcagagc ctctcagtat atacggaggc cggcacggca aatactgtaa gagccttgag
    10861 gctgacggga cgaaagcgac cctgaccttt gaaaggtata ttgccggtgc cgtaaaaacg
    10921 gtgcaggaga ctcccggagg agagccaatg gtatcactgc tcttcacctc tgacatggtc
    10981 agcagtgaaa ctgtggttct ggtgttggga aggacagtga tgataggtga catgaaggct
    11041 ggtttcggga ccccggctga catcaccgtg acaactgacg ggaatctgtt ctttatcagt
    11101 tcatccacgg gactcaggtc gacggcaatg caggagatga agtcggcaga acaccctgct
    11161 gactctcttg tgccactgat ggcaggcacc gtttacaccg ccggcagcta caggataatg
    11221 ccccggataa tgaccatgag cggtgccata gtgccctcgg tcactgcgga agaacaaagt
    11281 gccgggggtg cactggagtt ttccctgaag ggtgatggtt ttcaggaaac aatctggctg
    11341 tgggatgatg acggggaaga gcggtcggaa tggagcggcg atgccggcag gctgactgcc
    11401 agggtgagtt acggcgccag cctgagggaa ctgcccttca gcctcaggct tgacgatttt
    11461 atcgtggagc actatcctgg ctcaaacagc ccgtcaggat tcacgagcag ggtgattctc
    11521 agtgatccgg agcacggcac cgtaatgcct tatgacattt acatgaatca tatattgaaa
    11581 taccgcgggt atcgttttta ccagtcctcc tatgatagtg acgagcttgg tactgtcctg
    11641 tctgtcaacc atgatccggc agggatgtac accacttaca caggttatgg tttgctgttt
    11701 cttttcataa ttctctcact tctcaacagg aaatcattct tccgtacagt gactcccggg
    11761 cactgggcct cgaattaccg caaacctgct gctgcccttg ttttgattct tctgacctcc
    11821 ctgtcaccgc tgaagagtca gcatctggtg atagagcgga aggcggcaga cagtttcggc
    11881 gcagtgctgg cacaagacca gaagggcagg acaaaacccc tctataccat cagcagtgat
    11941 atcctgcgga aagtggcagg ggagaatagt tttgaaggga tgtctccgat gcaggtattc
    12001 cttggctgct catttgattt ttatcactgg caaaatgtgg cgcttataaa ggtttcaagc
    12061 gcggagctcc gccgtgagct taaccttgag ggtgaaaagg ctgctttttc tgatatcgta
    12121 aagatggcca aagatggcgg ttacctgctt gggccgcttg ttgacaaggc ctatgcaaag
    12181 agtgtgactg agcgcacgaa gtttgacaag gaagtaatta aggtggatga gagagtcaac
    12241 atctgctata tgatgtcgag gggagatttc atgcatattt ttccgctgcg cgacggcact
    12301 gaccactggg gaagcgctga ggaggctgtc aggcacggta tcgggcagga ggattctctc
    12361 tttgtgatta gtctcattcc gctgtgggct cagtctgtta cttcagtggg gcacccaatg
    12421 gctcccccgg cagactatat cacagcgctc accgactacc agcaccgtca tgccggttat
    12481 gaactgcctt cagagtgcaa ggtcaaggct gaactattct attataaggc caggatattt
    12541 gagaagcttt tcccatggta tgctgccgtt gggctgataa tgatcattgc cattattacg
    12601 atgattatca ggggcaggga actcagtgga gcggttctta aggttcttgc cctgctggtg
    12661 gctgccggtt tcctgtttca caccctcggg cttggcatca ggtggtatat ttccgggcat
    12721 tcgccgatga gcaacggtta tgaatcaatg ctgttcattt catgggttac attgctggcg
    12781 gggctgatct tcagcaggag gtcactattg actctggcgg ctacctctgt tcttggcgga
    12841 ctgacactga tggttgctca tctgagtttc atggatccgg agatcacgaa cctggtaccc
    12901 gtgttacgtt catactggct tacccttcat gtctccgtca tcaccggcag ctacggattc
    12961 ctgggtctgg gcgccatcct cggaataatc gttcttgtga tgatgctctt catccatgat
    13021 ggtaacaggg cgaggatcag tgccgtgatc gatgagctta ctgttatcaa ttacaggact
    13081 ctgaccctgg gactctattt cctgactatc ggcaccttcc ttggggctat atgggccaat
    13141 gaatcgtggg gccggtactg gggctgggac ccgaaggaga cttggtcact gatcaccatc
    13201 attgtgtaca ctctggtgac acattcgaga atgatgccgg ggatgaagga gacgtacatc
    13261 ttcaatctcc tttcgctgtt tgcgctctcg tcggtactga tgacctattt cggtgtcaac
    13321 tattacctgt cggggctgca ctcctatgcc ggcggcgatg cagtaccggt accggcgttt
    13381 gtttatgtgg cagtggcggt tctcatcagc ctggcggtag cagccggata taaatacagg
    13441 agagcgctga agcggcaggt ggggggcaat taacttcgaa ctaagcattc cgtatttttg
    13501 ataaatatga tataatagtc agaaaaagtt ggtaggattt ctctgtgata aagaacagat
    13561 gaggatttag gaaaagggga aataaaaagg ttattgattc ggtttaaaaa taaagctgac
    13621 actgatttaa tcagggtcag ctttatttgg cagggtggaa gaccggcctc gaaccggcga
    13681 cctccagatc cacaatctgg cgctctcacc aactgagcta caaccaccgt ttttgcggag
    13741 tacaaagaaa cagcaaaaat cccatttatg caagcagtca tttttacttt gttatcaatt
    13801 cgtgcaaata gtaactttgc cgcaccaaaa aacgggatgg tataattatt gtggcatcag
    13861 ggcatttccg atacataacg tgacagtttg agcaggctga agatcataga caacatctcg
    13921 ggactgcagg cagtacagct gctgagattc cttacctttc tcattatcag tatcgtattt
    13981 accaaaagcc atctcacccg ggcacagata ggcaactggg agctgtttct ctttatctcc
    14041 agcctgctca gcttcttctg ggtcaccggc ataatccagt cacttctcac cctctatgaa
    14101 cgcaaccgga ctttcaaaac cgacggaaca agggcgggga gaaaatcacc cgagatctac
    14161 aatgcgttcc ttctgatctc tttcttcagc ctgatggtat tcattctggg catacccatc
    14221 aggttcatgc ttgaatcatc acaggcagat actgacattc cctatcccaa cctgctgctg
    14281 ctttatatcc tggtcagcaa tcccgttgcc ctcattgaat acatctacct gctgaacaag
    14341 cgtcctcaca ggatactcca gtacggtatc tatacctact ctgcccagtt gctgctcgtt
    14401 ctggcgccgg tgattgccgg atacgatgag aagagcgcta tctgggggct ctttgcaatc
    14461 actgccgcgc ggtgggtctg gctcatcacc cttcttcgaa ggtacacgct cctcagggtg
    14521 tcgccggaat tcatcaggga acacctcagg gtggggatgc ctctcatcct tgccttcctg
    14581 ataagcggct cggcacagta cgttgacgga atcattgtct ccgcccgcta ttccgatccc
    14641 ggtgtctttg caatgtatcg ttacggggca aaggagtttc cccttgttct gcttcttgcc
    14701 aacggactga gcaacgccct cctgcctcag ttcagtacca gggaagggat gcgcgaagcc
    14761 ctggtgacac tccggaaacg gtctgacagg ctgatcaaat acctcttccc gatctcaatg
    14821 gttacgatgc tcgtggccag atgggtctat ccgcgcctct tcacccccga gtttacacgc
    14881 agcgctgacg tgttcatgat ctacctgctt ttgattattc caaggctggt attccctcag
    14941 accatcgtga taggaaggcg taaaaccaat gtgacactct atactgctac cattgaatta
    15001 gctgtaaaca tcccgctcag cctgctgctt ataaaaccat acggggtggt tggcgttgca
    15061 ctggcaacct tcattgtcta ttttctcgaa aagatattcc ttatctatta cgtgtggaaa
    15121 aaaatgaaga tcagacccgc ggaatatata cccctcaccc ggtggatcat ctattctgca
    15181 ctgctcatcc tgctatttgt gctgattgac caccgcatca tagacatcta ctgattcact
    15241 caggtaaact gaggaagtgc acatgcttca tacccaattc caaaaagaat tatatttggg
    15301 acgtattata acgcctgtaa aaaatggctt ttcacaacag cagtctggag gattattttg
    15361 gcacattcag aaggaatatt gtagggattg accagaacta catttctccg tacgggctta
    15421 agaagatcat ctattgtgac tggatagcca gcggaaggct gtacgggccg atagaagaaa
    15481 agataagcca tattatcggc cctttcgtcg ccaacacaca taccgaaacc tccgaaacgg
    15541 gttccatgat gacgtgggca tatcattatg cccaggagat aatcaagaag catgtgaatg
    15601 ccgggcctga cgacgtgcta attggcgcag gagccggcat gacggctgtc ataaacaaat
    15661 tgcagaggat actggggctc aagggttgcc tctaccccgg aaaggcaggc tgcctggacg
    15721 ctacagaccg tccggttgtc tttataaccc atatcgaaca ccattcgaat cagacctcat
    15781 ggtatgagac ccttgctgat gtggtaatca ttgaaccggg aagcgacctc acggttgacc
    15841 cggagaacct gaggaaagcg ctggaaaaat acagtgacag aaaattcaaa ataggatcat
    15901 tttcgggctg ctcaaacgta acgggggctt tcactcccta tcacaccctg gcacgcataa
    15961 tgcatgagca cggcggtctc tgcttcattg atttcgcagc ctcggctcca tatgttgaca
    16021 taaacatgca tccggatgac ccgatggaaa agctggatgc cattttcttt tcaccacata
    16081 agttcctggg cggaccgggg tcatcaggaa tcatgctgtt tgataagtca ttatacaaat
    16141 catcagtacc ggaccagccc ggaggaggca cggttgactg gaccaatccg tggggtgagt
    16201 acaagtatgt tgacaacatc gagtcaaggg aagacggcgg caccccggct tttctgcaga
    16261 tgatgagggc tgccctggca atcaggctga aagagcagat gggcaccgca aagataatgg
    16321 cccgcgagga ggagctcctg gccatgactt tcagcgggtt gtcggccata ccggggctgc
    16381 atatcctggc ttcaaatgtc aggcatcggc tgggcgcact ctccttttat attgacgacc
    16441 tccactataa cctggtggtc aagctgttga gtgaccgtta cggcatccag gtcaggggcg
    16501 gctgcgcctg tgcaggaacc tacggtcatt acctgcttga tgttacctat gaccacagcc
    16561 gccggattac agagatgatc aacaggggtg acctgtcaga aaaacccgga tgggtcaggc
    16621 tgtcaatcca tcccaccatg acagacgctg agcttgaaat ggttattgcc gccctggcag
    16681 aaatccgtga caatgctgct gaatggagta aggattatat atacagcaga cgcactaatg
    16741 aatttaccca ccgtgaccag accggtaccg gaatggagag agtaagcccg tggttcaacc
    16801 ttcagacctg aagacgatga aaataacaga agtaaccact gaatccctca gaaaggaatt
    16861 cattgatttt ccgaaaaagc tttacagggg agaccccaac tgggtctgcc ctcttgacag
    16921 tgagacagag gctgtcttta gccatgaacg caatgttgct ttcaggcacg gcgaggcagt
    16981 gcgctggatt ctctctgacg gagatggcaa caccataggc agggtggcgg cattcattga
    17041 tgagatacgc tcaaaggcaa acaggcagcc caccggaggc cttggctttt tcgaggtgat
    17101 agaatcgcgc gatgctgcct tccttctctt tgacactgcc cggcagtggc tgacggagag
    17161 agggatgaag gcaatggacg gccctatcaa cttcggagat aatgacaact actggggtct
    17221 gctggtggaa ggattcatgc agcccggctt cggaatgccc tacaacaaac cctactacaa
    17281 acactatttt accgattacg gtttccggac ctacttcgag caatacagtt accataagga
    17341 tgtagccgcg gtggaagtct ttcccgaacg gttcaggaag atagccgagt ggatttcaaa
    17401 gaggcccgga tactcttacc gccattttga cttttcgcag cgtgacaaat ttgctgccga
    17461 catggtggag atttacaacg ctacatgggc agtattcaag gaggacttca ccccgcttga
    17521 tccggcgagg atagaggaca ccctgcacca ggccagtgcc tttattgacc ccgagctgat
    17581 ctggtttgca taccataatg acaagcctgt ggcatttttc atcattttcc cggacctgaa
    17641 ccagatcatc aggcacttca acggccgcct gacattgctg aacaagatcc gtttcctctg
    17701 gtacaagatg actcatgaga tgaccagggt cagagcgttt gttgccgggg tacacccatc
    17761 ttaccagaat gccggcattg aatcggctat tttcctggag ctcttccgcg tcttcagaaa
    17821 gaagaggtac tacagggagc ttgagctctc atgggtagga gatttcaatc cgaagatgat
    17881 atcaatttac gaggctattg gcgctacaag ggcaaaggtc caccttaccc tgagatatat
    17941 gatagatgac tccctcccgt ttaaaatgta caaggacgag atggaggaga acagggccgc
    18001 cagacaggcc ggacagaaga agtgaatttt ttttgatact cttttgcatt ttctttattg
    18061 ttagctatct ttgcactccc ttgaaaaagg gatttttgaa cagtaattaa ttatacaaga
    18121 gatgaaagaa ggtatacatc cgaacaatta caggctggtt gtatttcagg atatgtcaaa
    18181 cggatacacc tttatgacac gttcaacggt cagttcacgt gataccatca aatgggagga
    18241 cggtaaggag tatccgctca tcaagcttga gatttcaaac acatcgcatc ctttctacac
    18301 gggtaagatg aagctggttg acacagcagg aagggtagac aagttcatga accgttacaa
    18361 ggatcatctt gcaaagaaga cgaagtaact gaaagtcaca tgatagaggg ctgccttaaa
    18421 caaggtggcc ctttttttgt tttattgttg catatgccgc ccggataagg aggatgccgg
    18481 cacagttaga gtaatattaa gtaaatttgc atacttttac tccatatgga aaaaaaggtt
    18541 tacggaaatt gggacaggat cggtttgcgt gagctgattg atgaggggag ggagataatc
    18601 cccatcctta ccgatggtga tgaccaggag ctggaggatg ttcagattcc ggggagtatt
    18661 cccctgttgc cgcttcgtaa caccgttctt tttccggggg ttgtgatacc cattgccgtg
    18721 gggcgttcca agtcggtgca gcttgtgcgc gacgcatacc gtggcgacaa actggttgct
    18781 gcagtggcgc agcttgacgc cgaggtggat gaccccgggg tgcgtgacct gaatgcggtg
    18841 ggaaccctgg gtcatgtggt aaagcttctg gagatgcctg acggcactac cactgccatt
    18901 atccagggca ggaaaagagt tgcaatagac aacataatcc agaccacgcc ctatatgacg
    18961 gcacagataa gggccatgcc cgaaccgagg ggagtggatg atgagaaaga cttcgaggta
    19021 ataatcggat cactgaagga tctctctgtc aagattgtca agctcaaccc gaacatcagt
    19081 cctgaggcca cctttgccgt gaggaatatt gagaacagca cctacctgat aaattatatc
    19141 tgtgccaaca ctgagatcaa cgtcactgac aagcagaagc tcctggaagc gggctcgatc
    19201 agggagcgcg ggctgaggct gcttgagcac ctcgttcgtg agatacagtt gctggagctg
    19261 aagaacgaac tgcagtcgaa ggtaaagtac gagattgacc agcagcagcg tgagttcctg
    19321 cttcatcagc agatgaagac tattcagaat gagctgggag gaagtcccat cgagaaggag
    19381 gttgaggagt ttcgctcccg gggagcgaag agagcctggg gtgaggatgt tgcgaaggtc
    19441 ttcaaccggg agcttgacaa gctgtcacgt ctcaatccgg ctgccgccga atactcggtg
    19501 caggtcaact acatccagac cctgcttgag ttgccctgga atgaatatac cactgataat
    19561 cttgatcttg agaatgccaa aagcgtactt gatgaagatc actacgggct tgaggaggtc
    19621 aaggagagga tactggaaca ccttgccgtg ctgaagctga agggcgacct gaagtcaccc
    19681 atcctctgtc tttacggccc gccgggagta ggcaagacct cgctgggcaa gtctgttgcc
    19741 cgggcactgg gccgcaaata tgcccgcatg tcgctgggcg gattgcatga cgaggcggag
    19801 atcagggggc accgcaagac ctacatcgga gccatgccgg gcagggtggt gcagaacatc
    19861 cgaagggcag gctcttcaaa tcccgtcttc atccttgacg agatagacaa ggttggacag
    19921 gatttcaggg gagacccgtc gtcggccctc ctggaggtgc ttgaccccga acagaacacg
    19981 catttctacg ataatttcct ggagctggag tatgatctct ccaaggtact gttcatagcc
    20041 acagccaaca cgctctcaac agtgagtccg gccctgcgtg acaggatgga gctgatagag
    20101 atatcgggat accttgttga ggagaaacgt gagatagccg tgaagcacct gatacccaaa
    20161 cagcttgatg cgcatggtgt gacaccggag cagttcatgc tcagccgtga ggttattgag
    20221 gaggtgatac agaaatatac gcgcgaatcg ggtgtcaggg agcttgacaa gaggatagcc
    20281 aaactggtca ggttcagggc acgtgagata gcctttgaaa acaagcctga accggagatc
    20341 acctctgcca tggtggagga gatacttggt ccgcctaagt acctgaagga tatttatgac
    20401 ggcaatgacc aggcgggcgt tgtcaccggc cttgcatgga ccgccacggg cggagagata
    20461 ctcttcgtgg agacaagcct cagccgtggc aagggcaatc tgacacttac cggcaacctg
    20521 ggtgaggtga tgaaggaatc ggccgtgatt gcccttgaat acctgaaagc gcatgcctca
    20581 ctcctcaacc tgccggagac cttcgcggag aaatggaata ttcacatcca tgttcctgaa
    20641 ggagccatcc cgaaggacgg tccttccgcc ggcataacca tggcagtttc aatagcttcg
    20701 gccttcacac agaggaaagt aaagaaatat gtggctatga ccggagagat aaccctgagg
    20761 ggaaaggtac ttcccgttgg cggcatcaag gagaagatcc tggcagccaa gcgtgccggc
    20821 ataaaggtga tagtgctctc tgaacataac cggaaggatg tggaggagat tcaggagaag
    20881 tatgtcaaag gactgaaatt caagtatgta agtacaataa tggatgtgct tgaccatgtg
    20941 ctgctgcagc agaaggtggc aaatccgagg ataattgaat ttgaataaca gcataatcag
    21001 gtcatggata aagttgctct tttcccgggt tcgtttgatc ctttcactgt aggtcatgaa
    21061 tcacttgtaa aaagatgcct caggctcttt gacaaaattg tcattgcagt cggggagaat
    21121 tccgacaaga aaaacctctt cagcacagag atgcgtatga agatgatctg cagagtattc
    21181 gagggagaga gcaggattga ggttacccgc tacagcggac taacggtgga attctgccgt
    21241 agttgcggag cacggtatat catcaggggc ctgcgtaccg cggcagattt tgaatatgag
    21301 agggcgatgg gtcagatgaa ccggaagatg gccccggaaa tagataccgt gttccttctt
    21361 accagcgccg agcatacccc gatcaactcc acaatagtcc gagatataat caaaaacggt
    21421 ggcgatgtct ccatgttcgt tcctgcagcg ataaatattt ctgaatacct ggggaaataa
    21481 gaggttctgc cggcactatt ttcacgggat gtccggtggc acagcggaca gccccggtca
    21541 ttttgcggca ggtcagagcc tgcggcacag ccccggtcat tttgcggcag gtcagagcct
    21601 gcggcacaag cctgattatt ataccctgag ggtaaaattc aacagatcaa gaagcgactg
    21661 gaatgtgttt gacttgattc ttgctatctc gtcaggtaca agccattgtg catgggtaat
    21721 tccctccgat gcctgagggt cagtgatcat ttctccgctg tagctcatcc tgaaccagct
    21781 tgtctttttc atgtaaggct tgttgtcaaa gaggtaggtg tggtagctcg gtggaagagt
    21841 tccctttatg atgtggccat gaatgttgca ctcttccgtg acctccctga tggcacactc
    21901 ttcaggtgtc tcattctcct ccatatgacc ct
//
LOCUS       DAOPBK010000016        21692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2561_length_21692_cov_6.067569, whole genome shotgun sequence.
ACCESSION   DAOPBK010000016 DAOPBK010000000
VERSION     DAOPBK010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21692
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2561_length_21692_cov_6.067569"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PLA17_01830"
     CDS             complement(<1..660)
                     /locus_tag="PLA17_01830"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPK84181.1"
                     /translation="MKKINETDNWNDREWAAAAAWLSGEDNGDGENARILAGEEADIM
                     KKWNDLKYSEEKPVDIDKAWVKLNGRIEAAERPVMPLPFKRTPFLTTIARIAAMVLIV
                     AALGWLLFEVAAPQKITLTSDSDQKNLEALLSDGSTVFLNRNSSLTYPKNFMSGTRKV
                     SLKGEAFFDIVPDEEHPFIIDAGKAKIQVLGTSFNVITENADNEVEVYVASGKVMVTS
                     VD"
     gene            complement(660..1193)
                     /locus_tag="PLA17_01835"
     CDS             complement(660..1193)
                     /locus_tag="PLA17_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HPK84182.1"
                     /translation="MFSDSEIIERIRQGDKQEFEKLFRSCYASLVRYAKTLIRDHDAS
                     EEIVQELFFRLWQDRQAIRIESSLNGYLYKSVYNRALHYLEHQKVISRHAGEVLAAAD
                     SASEPVTDAIYYHELQARVARVLERLPERSRLIFRMNRFEGLKYNEIADKLAVSLKTV
                     EADMGKALREFRKALAE"
     gene            complement(1212..2513)
                     /locus_tag="PLA17_01840"
     CDS             complement(1212..2513)
                     /locus_tag="PLA17_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPK84183.1"
                     /translation="MKTKVIFTAIVSALLLIVTAGDMPVLAAPAKSVKESPQQVITIR
                     GRIVDAETNQPLVFAGITVQGSNISTVTNLDGEFTLKLPGTETGNLEFSFIGYKNRVM
                     AIEEMKTNGQRNIIRLETALIPIREVVVKPLVPEEIIEQVIRRFDENYPGVANDMTGF
                     YRETIRKNRSYISIGEALVEIFKAPYQNSLRWDAVRIYKGRKSNDVEKMDTVLFKLQG
                     GPTTTLYLDVVKNPEMFLTREALTQYDLELSNIAMIDDRSNYVINFYQKPSITTPLYQ
                     GRLFIDIETYAVAQAEFAFNLENKELATSMFIRKKPVGMQVTPELTSYLVRFREQDGK
                     WYFNHSRAEVKFKVDWKKKLFNTNYTTMSELAITDRTEEDVVRFSNKERIKPTDFFTE
                     EVSAFADPDFWGDYNVIEPDQSIETAIRRLSRKVKFSDRKE"
     gene            2624..3592
                     /locus_tag="PLA17_01845"
     CDS             2624..3592
                     /locus_tag="PLA17_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HPK84184.1"
                     /translation="MAHGYLNASYSVFSYAAKRYGGIRAIPPLPPAISVELSSVCNLA
                     CPECVTGLGLTKRANSFIGYDLAAEIASQSGGRVLSAWLSFQGEPMMHPEFFRITELF
                     AGMNPVISTNGHFLNIENCRRLAASPLKKIIISLDGATPEVYTLYRKEGDHAVVMEGI
                     RRLSGTIREMKSKLKVVIQFLVHRGNEHEAEAVSSFARSVGAGFRVKSMQVLDPAGAG
                     KWVTSDPRKSRWVKGDDGAWRAAESSARGCFRMWSSVVITADGDVIPCCYDKELRHVM
                     GNLERQTLSAIWRGERYNSFREKVMSNRRQVDICSACPEGRRLFFK"
     gene            3660..6050
                     /locus_tag="PLA17_01850"
     CDS             3660..6050
                     /locus_tag="PLA17_01850"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPK84185.1"
                     /translation="MKILKITLVTIAVLLAVVLLAGVIAVPALRKSGLPELNGEKSLS
                     ALTAEVKVIRDERGVSHIYASNEHDLYFMTGYITAQERLWQMDMVRHATQGRLSELFK
                     RDMFETDIFLRALGMQEKSRMVLEKEDPEILATLQAYADGVNAWITGCGKKLPPEFRV
                     LGYEPEPWTMVDITNIIGFIGWDLASSNLSNEISNYKLGMKLGAEKAAELIADWNLVD
                     EVVFPDFRLDDKLTDKALKAVSSMEKLEELGIVTLSGSNNWAVSGSRSETGKPILSND
                     MHLGFNVPGFWLQVHQVIPGKLNVTGVLFPGEPFIIAGHNDRIAWGMTNLGVDDIDLF
                     VETVDSTGNNYLYNGEWLPFRDVEHTLKMTDDSSQTRVLRYTHHGPVISGMQNIDDVV
                     LSMCWSGYDYSDEIKAVYLLNRAGNWDEFRTALSHFKSISQNFAYADVDGNIGLNTGG
                     GVPVRKGTGLLPRRGDTDEYEWKGYVPFELLPSSFNPEEGFISSANQRTVDSSYPFFI
                     SGEFSMPYRIMRIREMAGEKQVLGIEDFKRMITDNHSAYAKMLTPVILKGAEALSDPG
                     ETERKAIEELRVWNYAMDASLVAPTLFEFIRMEMAYQLLGDELDELYGAALGRQHDFY
                     LYRLMKEGPDGWVDNVNTPEEESLETIIARSISAALDTLTARYGEYGEQWQWGRIHTI
                     TLEHPMGGVKILDRVLKLNSDTYGVGGSYHTVEPYAFRDNFKAHHGASERHIFNTADW
                     DKSLTVIPTGTSGIPGSPFYLSQTETYINNGFYSEPFSDAAVEAAKKYEMIFRPGK"
     gene            complement(6100..6663)
                     /gene="frr"
                     /locus_tag="PLA17_01855"
     CDS             complement(6100..6663)
                     /gene="frr"
                     /locus_tag="PLA17_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPK84186.1"
                     /translation="MTETLELIRVEAEDKMGKAIKYLEYELSHLRAGRATPLLLDGIN
                     VDYYGTMTPLAQVSNINTPDARNIIIQPWEKSMLGVIEKAILAANIGMTPMNNGEIIR
                     INVPPLTEERRHQLVKQVKQEGETAKISIRSGRKWFLDELKALLKEGLPEDEEKTAEK
                     LIQELTDKHIAMVDKIVETKEKDIMTV"
     gene            complement(6708..7415)
                     /gene="pyrH"
                     /locus_tag="PLA17_01860"
     CDS             complement(6708..7415)
                     /gene="pyrH"
                     /locus_tag="PLA17_01860"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPK84187.1"
                     /translation="MKYKRILLKLSGESAGGPDGTGLDAAVLSGYARQIKDVVDAGCQ
                     VAIVIGGGNIFRGLGSIGEGYDRVKGDQMGMLATVINSLALESFLSRADCNVKVFTSI
                     RMEPVGELYSRDRASAALDCGCVTILAGGTGNPFFTTDTAAALRAVELNCDVLLKGTR
                     VDGVYTADPEKDPSAVKYDSLTFGEAIEKKLRVMDQTAFSMCAENGMPLIVFNMNREG
                     NLMAVAKGNRCGTVINL"
     gene            complement(7545..7748)
                     /locus_tag="PLA17_01865"
     CDS             complement(7545..7748)
                     /locus_tag="PLA17_01865"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPK84188.1"
                     /translation="MMKMNIGKTDRSVRLILAVLLVILYFFVGKITGLIFIVLAIILA
                     VTALTRTCPLYYIFRTDTLDKKE"
     gene            complement(7972..8400)
                     /locus_tag="PLA17_01870"
     CDS             complement(7972..8400)
                     /locus_tag="PLA17_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK84189.1"
                     /translation="MMKIREIKRYSREAFKAVSDLIPQLSPESPPLSEEHFRKIIRTK
                     GTHLFVAEYRNRIIGMFTLATYPIPTTDKYWIEDVVIDKSVRGKGFGREIINYAIEFA
                     RQQGAENVNLTSRPFRTEANRLYKSIGFTLRETNAYRYNF"
     gene            complement(8535..12863)
                     /locus_tag="PLA17_01875"
     CDS             complement(8535..12863)
                     /locus_tag="PLA17_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPK84190.1"
                     /translation="MNSSLSHINSSAQNPIKPLINIGLDAGSTTIKVVVSDPQNKIIY
                     KDYRRHYADVLGNLKQILSDMLDKIGDCPVKLCITGSAGMGIAERYEVPFVQEVVASC
                     EVILRQFPEIKTFVDIGGEDSKMIFFKSGKTPDIRMNGSCAGGTGSFIDQMATLLNVT
                     MDEFNSLAEQAETIYPIASRCGVFSKTDVQNLLARNASKADIAASVFRAVSMQVITSL
                     ARGYEPEGKVFLCGGPFTFIPYLRKVFIDEIKMDHSEVVISENPEVTPAWGAAIIASD
                     RQESRLLSEYLSLFGEEVKENPKDAHNQLKPLFKDKQEYTEWLKSKEEYQFPAIDIKE
                     IKNPNCFIGIDSGSTTTKIIATDENARVFYHYYTKNKGFSLQAVTVGLQKLFKLTQEA
                     GIEMNVLGSCVTGYGEDLIKKAFHLDSGMVETIAHYMSACHFNKDISFILDIGGQDMK
                     AAFIENGVIKRVEINEACSSGCGSFIETFADSLNYSVSDFSKIALKSKHPCDLGTRCT
                     VFMNSKVKQSQREGASVEDISAGLGYSIVKNCLNKVLKLKDIKELGSHIMVQGGTFRN
                     LSVIRALEIELGKEIMVTDYPELMGAYGAALYARKKFETGQTRPVRLAAMAESREYSQ
                     KRTVCKGCENNCTVTHFRFDNDNVFYSGNKCEKVFNNSGETVAKGANLHTEKYDLLFN
                     RPVLEKGKITLGIPRTLGMYENYPFWHSLLTGCGIRIVLSDKSTMAMYETGISTVMAD
                     NICFPAKLVHGHIYNLADKKVDRIFMPYVVFEDKEDDKTSNSYNCPIITGYSDVIKSA
                     VNPKHKFGIPVDAPTFTFANKKLMRKSCVEYIKTLIPGTDTKTIERAFRNALVAQQSY
                     EEQLNRRSNQILERAVAENRLVILLAGRPYHIDPLIQHKISDLVSDFGADVITEDVVR
                     HLQANPEEVQSVMQWAYTNRIFKSALWTANEAPQNVHYVQITSFGCGPDAFILDEVID
                     ILHRKGKNATIIKVDDINNIGSTRLRIRSLIESLKFKKEDEVFEKDIAVHTPPFEKED
                     RRRTILGPWFGDFYSPFIPAAFELMGYKMENLPPSDKKSVEYGLKYSNNEICYPATLV
                     VGDLMKALESGRYGRNEVALGITQTGGQCRATNYVALIKKAMIAAGFEDIPVVTMSFT
                     AGLNEQPGFRVKWEKYIRAIFCAIVYADCISQFYYSTAPRETEIGGAKRLKEKYLNLG
                     IEALKNNEANRFFHLAEQAADDFLSINSLKEIPRIGIVGEIYVKYNDFGHKHVVNWLV
                     KQGVEAVVPPLIQFFISTFASQEARIQGNVKERTIPKFAMNFVENLAYRIIRKMEAGI
                     LHYPYYFPISNVHEGARDASRIISTNAQFGEGWRIPAEFSEFAHNGINNVISLQPFGC
                     IANHIISKGIEKRTKELFPNMNLLFLDFDSGMSEANIYNRLHFMIRNAQLEVPSVDQH
                     ELVDAV"
     gene            complement(12907..13545)
                     /locus_tag="PLA17_01880"
     CDS             complement(12907..13545)
                     /locus_tag="PLA17_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPK84191.1"
                     /translation="MSQLSDSEVKIIEAAKQVFLEKGLENAKMRDIADTAGISRTALN
                     YYYRTKENLFYKIIEEILEGIIPHFESLLSKDTLSFQERLLGIIDVYADFLSRNNAVP
                     RFVMMEMQRHPQTLFDFINTNQKVKEYLQLIHAMLDREAEEKSIKQLPMEELVTLFYG
                     LLFVPYLVDPFIQEFFLNEKSKIEAYHRNHLENMKQVLLLFLSVKSLNQTVN"
     gene            complement(13864..14694)
                     /gene="tsf"
                     /locus_tag="PLA17_01885"
     CDS             complement(13864..14694)
                     /gene="tsf"
                     /locus_tag="PLA17_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPK84192.1"
                     /translation="MSNISAADVAKLRRMTGAGMMDCKKALEEAAGDFEKAQELIRKR
                     GQAIANKRADREATEGVVLAKTTADGKTGMMISLNCETDFVAKNADFIDLGYKILDGA
                     LKSAPADLEALKNTVIDGKKVSDLVLEKSGITGEKFDLTYFGTITAEAVQPYIHQGNK
                     LATLVGFNKAGIDMQVYKDVAMQVAAMNPVSVDKDDVPEKILAQELDIAREQARREGK
                     PEEMIEKIAQGKLNKFFKESTLMNQEFIKESKINIRQYLESHDKSLRATGFLRYTLNV
                     "
     gene            complement(14726..15601)
                     /gene="rpsB"
                     /locus_tag="PLA17_01890"
     CDS             complement(14726..15601)
                     /gene="rpsB"
                     /locus_tag="PLA17_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPK84193.1"
                     /translation="MSRTNFKELLDAGVHFGHLKRKWNPAMAPYIFMERNGIHIIDLE
                     KTLIKIEEAAAALKQIAKSGRKVLFVATKKQAKEIVAEKVGAVNMPYVTERWPGGMLT
                     NFPTIRKAVKKMSSIDKMATDGTFDNLSKREKLQITRQRAKLEKNLGSIADMTRLPSA
                     LFVIDVCKELIAVHEAKRLGIPVFAMVDTNSDPSDIDFVIPANDDAAKSISLVVDIMC
                     QAIAEGLNERKAEKDKESSQTDREEMPEGSRKGKFRAADYEEDKPEMKDDDDIEDSAD
                     KDEEEEVEESIEEEE"
     gene            complement(15717..16103)
                     /gene="rpsI"
                     /locus_tag="PLA17_01895"
     CDS             complement(15717..16103)
                     /gene="rpsI"
                     /locus_tag="PLA17_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPK84194.1"
                     /translation="MEVINAIGRRKAAVARVYLRDGKGQIMINEREYKQYFQNEILQY
                     VVLQPLNLLNVADKFDITVTLDGGGSKGQAEAVRLGISRALIKVDPEFKSKLKAEGFT
                     TRDPREVERKKPGRPKARKRFQFSKR"
     gene            complement(16109..16564)
                     /gene="rplM"
                     /locus_tag="PLA17_01900"
     CDS             complement(16109..16564)
                     /gene="rplM"
                     /locus_tag="PLA17_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPK84195.1"
                     /translation="MNTLSYKTVSANKATVEKEWVLVDAEGQALGRLASVVALMLRGK
                     HKTSFTPHVDCGDNVIVINAEKIALTGAKMTDKEYIRHTGYPGGQRIVRADALMKKNP
                     ARLIEYAVKGMLPKNRLGAAIFKNLYVYVGPEHKHEAQKPKPIDLKTIK"
     gene            16691..>17406
                     /locus_tag="PLA17_01905"
     CDS             16691..>17406
                     /locus_tag="PLA17_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84196.1"
                     /translation="MFSRNIHQTVHLICLLLAAFMMPLSVWLLSVVSIIMSVNWLLGG
                     EYRHKINILRSRPGVLILSGLFAMYLVWLLNTTDFHGAVAELRLKLPLLFFPVVAGST
                     ESAERSLLRMVLLSFIAGCLFATVAGFAALAGLTPGEIHSSRELALFVPSIRLSILLC
                     FAIFSSLWLIIYDSAGQGKAGSHLPEWWQRLNSRLLPGTGGRLYVVMLAAAAMSYFLF
                     RLLSVTGLVIFVLLLFLTAV"
     assembly_gap    17407..17416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17417..18356
                     /locus_tag="PLA17_01910"
     CDS             <17417..18356
                     /locus_tag="PLA17_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK84197.1"
                     /translation="GGRRVRWGITFLAVAATITAALTIVSLSAWNRLHNPADPSVNGK
                     RQLTLSGRAYTHYPEETLIENGYLVWVNVCEEELRQEWNRRSLKPYDGADAAGNELRV
                     TLVRYITAKGMAKDSAAVAALTDRDIFNIEQGYANPLYARKGSPMARAYEIAWELDRA
                     ASGANPSGHSLTQRPEFYRAAAGIIRKHPWTGVGTGDLARAFSDEYISISTPLEPEYR
                     LRAHNQYLTFGVTFGIPGMILAITLLLIPWLINDGRFSYLFLVFMTLILISMFNDDTF
                     SSFTGASFFSYFYTLLIVTSAGKEYIHHSRQCGEEC"
     gene            18375..18854
                     /locus_tag="PLA17_01915"
     CDS             18375..18854
                     /locus_tag="PLA17_01915"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPK84198.1"
                     /translation="MRLNIKDRKYLELAIKTAGENMEDGGGPFGAIITKGETILARAG
                     NRVVPGHDPTAHAEVIAIRMAAEKLGTHDLSDCTIYASCEPCPMCLGAIYWAGIRRLV
                     YSSDRYTAASAGFSDNHIYEELALDNDSRSIEMVRGLKKEGDNVLKRWVDLPDKIQY"
     gene            18864..19388
                     /locus_tag="PLA17_01920"
     CDS             18864..19388
                     /locus_tag="PLA17_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPK84199.1"
                     /translation="MGKVDRDFYLADAVTVARQLPGMELVMASGEQCLRYVITETEAY
                     LGEGDRACHASRGRTRRTEPMFLTGGHLYLYFVYGMHWMLNVVTGPADHPQAVLIRGV
                     SQYQGPGRVTKGLGIDGSFNREDLTSSGRIWIEDTGFRPELISGPRVGIDYAGEPWIS
                     KPWRFIVRNEKEKI"
     gene            19385..>21692
                     /locus_tag="PLA17_01925"
     CDS             19385..>21692
                     /locus_tag="PLA17_01925"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPK84200.1"
                     /translation="MKKGFRLWNSITGWAVFAVSLVIYLMTIEPTVSFWDCGEFILCS
                     YRLEVGHPPGAPFFMLLGRFFTLFAGGDTSKVAMMVNILSALASAFTILFLFWTITRL
                     VRLATGDDETVTGGKSLAILAAGAIGALAYAFSDTFWFSAVEGEVYATSSLFTAAVVW
                     AMLRWYDEADDPRSGRWIILIAYLMGLSIGVHLLNLLALPVIVLIWYFRKHKATTRGF
                     IYALLAGFVILGVLNFVFVPGIPTVAGWFELFFVNTIGLSYNTGLYIYLIILTGLIAG
                     GIWYSLKKGRRILNYVMTVIAVLVLGYSSFAMIIIRANAHPPMNQNDPSDVFSFIYYI
                     NRSQYPRAPFLYGNYYSAPVIGLEKKIGGYNKKDGRYEPYYTTEYKYDKRFMTIFPRM
                     YSTDQGHIREYEYWGKVRGKKITVRSGQGDEQVTLPTFGENLRFFFRYQVGVMYMRYF
                     MWNFSGRQNDIQGNGNVMDGNWITGIPFLDNPRLGDQSLLPDDYRNNPARNAYFLLPL
                     LAGLAGLYWQYRRDGKGLSLVLLLFLMTGLAIVTYLNQNPSQPRERDYAYAGSFYAFA
                     IWIGMSVMLFYDLLRKTMKDHPAAILPSVLLLAAVPLLMLVRNYDDHDRSDRYTARDI
                     ASNYLATVDENAVLLTYGDNDSFPLWYIQDVEGFRTDVRVANLSYLQSGFYIETMSRK
                     AFESDALPFTIPVEKYIEGARVQLPVRELIKEPTPIRKVMEFVGSDDPKAMVDLSGRG
                     DYFNFIPVTEFIVDVDSAHVIATGAVRPY"
BASE COUNT         5167 a         5162 c         5205 g         6148 t
ORIGIN      
        1 atccacgctt gtcaccatca ctttaccgga ggcgacatac acttccacct cgttgtcagc
       61 attctcggtg atcacgttga acgatgtgcc cagtacctgg atttttgctt ttcccgcatc
      121 gatgatgaac gggtgttctt catccggtac gatgtcgaag aaagcttccc ctttcagcga
      181 tactttgcgc gttccgctca tgaagttctt cgggtaggtg aggctgctgt tccggttaag
      241 gaagacggtg ctgccgtcag agaggagcgc ctccagattt ttctggtcag agtctgaagt
      301 cagtgtgatt ttctgagggg cggccacctc aaagaggagc catcccaaag ctgcaacgat
      361 aagtaccatt gcagcaattc ttgcaatagt tgtaaggaag ggagtccgtt taaatggcag
      421 gggcataaca ggcctttcgg ctgcttcgat tctgccattc agcttcaccc atgctttatc
      481 aatatcaaca ggtttctctt ctgaatattt caggtcattc catttcttca ttatatctgc
      541 ttcttcaccg gccagtatgc gggcattttc gccgtcaccg ttatcctcac cggatagcca
      601 tgctgcagca gcagcccatt cccggtcatt ccagttatct gtctcattaa tctttttcat
      661 cactctgcaa gtgcttttct gaactcccta agtgcctttc ccatgtctgc ctcaaccgtt
      721 ttgagcgaga cggcaagctt atcagcaatc tcattgtact taaggccttc gaagcggttc
      781 atccggaata tcagcctgct tctttcgggc agtctctcga gcacccttgc cactctggcc
      841 tggagctcat gataatagat tgcatctgtc accggctcgg aagctgagtc tgctgcagcc
      901 agcacttcgc cagcgtgacg gctgatcacc ttctgatgct caaggtaatg caatgccctg
      961 ttgtatactg atttgtaaag gtaaccgttc aaagaacttt caattctgat ggcttgcctg
     1021 tcctgccaca gcctgaagaa gagttcctga actatctcct ccgaggcgtc gtggtccctg
     1081 ataagggttt tggcatagcg taccagtgaa gcatagcagg accggaacag cttctcaaac
     1141 tcctgtttgt ctccctgtct gatcctttct atgatttcac tgtcgctgaa catgggcaga
     1201 accccagggt tttactcttt tctgtcactg aatttaactt tcctgctgag ccttcgtatg
     1261 gctgtctcga tgctctggtc aggttcaatc acgttgtagt caccccagaa atcagggtca
     1321 gcaaaagctg aaacttcctc cgtgaagaag tctgttggtt ttatcctctc cttgtttgaa
     1381 aacctcacca catcttcttc ggtacggtca gttatggcaa gctctgacat ggttgtatag
     1441 ttggtattga agagcttctt tttccagtct accttgaatt tgacctcggc cctggaatgg
     1501 ttgaaatacc actttccatc ctgctcacgg aaccggacca gataggaggt cagttcggga
     1561 gttacctgca ttccgacggg tttcttcctg atgaacatcg atgttgcgag ctccttgttt
     1621 tcaaggttga aggcgaactc agcctgcgct accgcatagg tttcgatgtc gataaaaagg
     1681 cgaccctggt aaagaggtgt ggttattgaa ggcttctggt agaagttgat gacatagttg
     1741 gaacggtcat caatcatggc gatattggag agctccagat catactgggt gagggcttcc
     1801 cgggtgagga acatttcggg gttcttcaca acgtcaagat agagagtggt ggtaggcccg
     1861 ccctgcagtt tgaagaggac ggtatccatc ttttccacgt cattgctctt tctgcccttg
     1921 taaattctga cagcatccca cctgagactg ttctggtatg gtgccttgaa aatttcaacc
     1981 agggcttcac ctatagagat gtaagaacgg ttcttgcgta tcgtttcgcg ataaaagccg
     2041 gtcatgtcat tggctacacc aggatagttc tcatcaaagc gtctgatgac ctgttctatg
     2101 atctcttcag gtacaagagg ttttacaacg acttccctga tggggatcaa tgctgtctca
     2161 agtctgatga tgttgcgctg tccgttggtt ttcatctctt caatcgccat caccctgttt
     2221 ttgtagccta tgaatgagaa ctcaaggttg cctgtttcag tgccggggag tttcaatgtg
     2281 aattccccgt cgaggttggt gacagtggag atgttgcttc cctgtacggt tattcctgca
     2341 aatacaaggg gctggtttgt ctcggcgtca acaattcttc cgcggatggt tatcacctgc
     2401 tgggggctct ccttcactga ttttgccggc gcagccaaaa ccggcatgtc gcctgcggtc
     2461 acaatgagga gcagtgccga aacaattgct gtaaagatga cttttgtttt catagtctta
     2521 tttttttttg ttcttaatcc ggatcattgt tccatattaa tgaccggatc agcccctttt
     2581 cccctataca atcacaggaa aaaattgttc atttgccttt gccatggcac atggatacct
     2641 gaatgcaagt tactctgttt tctcttacgc tgcaaagaga tacggtggta tccgtgccat
     2701 tccgccgctg ccgccggcaa tcagcgttga gctgagcagc gtgtgcaacc tcgcctgtcc
     2761 tgagtgtgtc accggcctcg gccttacaaa gagggcaaac agcttcatcg gttatgatct
     2821 tgccgcggag atagcctctc agtcaggggg acgggtgctg tcagcctggc tcagcttcca
     2881 gggagaaccc atgatgcacc ctgaattctt caggataacg gaactttttg ccggaatgaa
     2941 tccggtgata tcaaccaacg gccatttcct caacattgag aattgcagaa ggcttgctgc
     3001 ttcgccgctg aaaaaaatta tcatctcact ggatggcgcc accccggagg tttacactct
     3061 ctaccggaag gagggagatc atgctgttgt catggagggc atcaggagac tttccggaac
     3121 aataagggag atgaagtcaa aactgaaggt tgtcatccag ttcctggttc acaggggtaa
     3181 tgagcatgaa gctgaagcgg tctcttcctt tgccaggtca gtgggtgcag gcttcagggt
     3241 aaagagcatg caggtgcttg acccggccgg tgccgggaaa tgggtgacat ctgacccgcg
     3301 gaagtcgcgc tgggtgaaag gcgatgacgg tgcctggcgg gctgcagagt catcagccag
     3361 aggatgcttc aggatgtgga gctcggtcgt aatcactgct gacggcgacg ttattccctg
     3421 ctgctatgac aaagagctaa ggcatgtgat gggaaacctt gagcgacaga cactcagcgc
     3481 tatatggaga ggagagagat ataattcatt cagggagaag gttatgagta accgccgtca
     3541 ggtggacatc tgcagtgcct gcccggaagg gaggaggctt ttttttaaat gagcagcaga
     3601 atgcctattg ccatgtggca ttcaattcgt aattttaaac aaatcaaacc ggaggttata
     3661 tgaagatact gaaaatcaca ctcgttacca ttgctgttct actggcggtt gttctgctgg
     3721 ccggagtgat agctgttccc gcccttcgca agtcgggcct tccggaactt aacggagaaa
     3781 aaagtttatc ggcccttacg gccgaagtga aggtaatccg cgatgagcgc ggcgtatctc
     3841 acatttatgc ctcaaatgag cacgatctct atttcatgac cggctacatc acagcccagg
     3901 agaggctctg gcagatggac atggtaaggc atgccacgca gggcaggctg tctgagctgt
     3961 tcaagaggga tatgtttgaa accgacatct tcctgcgtgc cctcggaatg caggagaagt
     4021 cgcggatggt gctggagaag gaagatcctg agatactggc aacccttcag gcctacgctg
     4081 acggggtcaa cgcatggatc accgggtgcg ggaaaaaact accgcctgag ttcagggtac
     4141 tgggatatga gccggaaccg tggaccatgg ttgacattac caacataatc gggtttatcg
     4201 gctgggacct ggcctcgtca aacctctcga acgagatcag caattacaag cttggcatga
     4261 agctgggcgc tgaaaaggct gcagagctca ttgctgactg gaatctggta gacgaggttg
     4321 tctttcccga tttcaggctt gatgataagc ttacagacaa ggcgctgaaa gcagtcagct
     4381 ccatggagaa acttgaggag cttggcattg tcaccctctc gggaagcaat aactgggccg
     4441 tgtcaggaag caggagcgag acggggaaac cgattctttc aaatgacatg caccttgggt
     4501 tcaatgtccc cgggttctgg ttgcaggtgc atcaggttat tcccggaaaa ctcaatgtca
     4561 ccggcgtgct tttcccggga gagcccttca tcattgccgg acacaatgac aggatagcat
     4621 ggggaatgac caacctgggc gttgatgaca tcgacctctt cgttgaaacg gttgattcaa
     4681 caggcaataa ttacctctac aatggcgaat ggcttccctt ccgtgatgtg gaacacacgc
     4741 ttaagatgac tgatgactca tcacagacaa gagtattgag atatactcat cacggccccg
     4801 ttatctcagg aatgcagaac attgacgatg tggttctctc aatgtgctgg agcgggtatg
     4861 attacagtga cgagataaag gcagtctacc ttctcaacag ggcaggcaac tgggatgagt
     4921 tcagaacggc cctcagccat ttcaaatcga taagccagaa cttcgcctat gctgacgtcg
     4981 atggcaacat agggctcaat acaggaggcg gagtgcctgt cagaaaggga acgggacttc
     5041 ttccgcgaag gggagacacc gatgagtatg aatggaaagg ctatgtgccg tttgagctcc
     5101 tgccttcatc attcaatcct gaggagggat ttatctcatc agcaaaccag aggacggtgg
     5161 acagcagtta tcccttcttc atcagcggcg agttttcaat gccataccgt atcatgagga
     5221 taagggaaat ggccggggag aagcaggtgc tgggaataga ggattttaaa agaatgatca
     5281 ccgacaatca ctcagcctat gcgaagatgc ttaccccggt gatattgaag ggggctgagg
     5341 cactcagcga tcccggcgaa acggaaagga aagcgattga ggagctgagg gtatggaact
     5401 acgcgatgga tgcttcgctt gtggccccga cgctttttga gttcatccgc atggagatgg
     5461 cataccagct cctgggtgat gagctggatg agctctatgg agcagcactg ggcagacagc
     5521 acgacttcta tctctacaga ctgatgaagg aggggccgga tggatgggtt gacaatgtca
     5581 acacacctga agaggagagc ctggagacca tcattgcccg cagcataagt gcggcgctcg
     5641 ataccctcac tgccaggtac ggcgaatatg gtgagcagtg gcagtggggc aggattcaca
     5701 ccataaccct tgaacatccc atgggagggg taaaaatact tgacagagtg cttaaactca
     5761 actcggatac ctacggtgtg ggaggcagct accataccgt tgagccctat gcattcaggg
     5821 acaatttcaa ggcacatcac ggcgcatccg aaaggcatat tttcaatacg gctgactggg
     5881 ataagtcact cactgtcata cctaccggca cctcaggcat accgggcagc ccgttctacc
     5941 tctcacagac agaaacatat atcaacaacg ggttctacag tgaaccattc agtgatgctg
     6001 cagttgaggc agcaaagaaa tacgaaatga tcttcaggcc gggcaaatag cctgccggca
     6061 caggtattga acaaaaaaag gggtgttgtg cccctttttt tatactgtca ttatatcttt
     6121 ctccttggtc tccactatct tgtcgaccat ggcgatgtgt ttgtcagtca gttcctgaat
     6181 gagcttttct gcagtttttt cctcgtcttc aggcagtcct tccttcagca gtgctttaag
     6241 ttcgtcaagg aaccatttgc ggccggaccg gatgcttatc ttggccgttt cgccctcctg
     6301 tttcacctgc tttaccagct gatgacgcct ttcttccgtc aggggaggca catttatacg
     6361 gatgatttcg ccgttgttca ttggggtcat gccgatattg gctgcaagaa tggccttttc
     6421 gatcacaccg agcatactct tttcccatgg ctgtatgata atgtttctgg cgtcaggggt
     6481 gttgatattt gacacctgtg ccagcggggt catggtaccg taatagtcta cgttaatacc
     6541 gtcaagcagc agcggggtgg ctctcccggc gcgaaggtgg gagagttcgt actcgagata
     6601 cttgatagct tttcccatct tatcttcagc ctcgactctg atcagttcca gtgtttcagt
     6661 catatcgttg tttttattct ggtgtaaaca aaagtaggaa aaacttttca gagattaata
     6721 acggtaccgc accggttacc ctttgcaaca gccatcaggt tgccctcccg gttcatgttg
     6781 aaaacaatca gcggcatacc gttctcggcg cacattgaga atgctgtctg gtccatcacc
     6841 ctgagctttt tttcgatggc ttcgccgaag gtgagtgaat catatttcac tgcggaagga
     6901 tccttttccg ggtcagcggt atagacgccg tcaaccctgg tgcctttcag gaggacatcg
     6961 cagtttagtt ccacggcacg aagggctgct gccgtgtcag tggtgaagaa ggggtttccc
     7021 gttccgccgg ccagtatggt cacgcagccg cagtcgagtg cagctgaagc cctgtctctg
     7081 ctgtacaatt ctcccacagg ctccatgcgt attgaggtga acacctttac gttgcagtct
     7141 gcccgtgaca ggaagctctc cagtgcgagg ctgttgatca cggtggcaag catacccatc
     7201 tgatcaccct tgacgcggtc atagccttcg ccgatgcttc ccagtccgcg aaaaatgttt
     7261 ccgccgccga ttactatggc tacctggcat cctgcatcaa ccacatcctt tatttgcctt
     7321 gcatagccgg aaagcacggc agcatccaga ccggtaccgt cagggccgcc tgcggactca
     7381 ccgctgagtt ttagcagaat tcttttgtac ttcataataa tgttccgttt aatgatggaa
     7441 acgaaattag caaaaaatgt atccggaagg tcatccgtga aaaaaaacaa agcggcgctc
     7501 ccgcgaggat acgccgctgc atgcccgtaa agcgggaaat gccgttactc tttcttatcc
     7561 agtgtgtctg tcctgaatat gtaatacagc gggcaggtac gggtcaaagc ggtaacagcc
     7621 agaatgatgg caaggacaat aaatatcagt cctgtgatct tgcctacgaa aaaatagaga
     7681 ataaccagca gtactgccag aatcagtctg acagacctgt ctgttttacc gatgttcatt
     7741 ttcatcatag tataattttg atttttaagg tattattgag accacatgac tgaatataat
     7801 gcaaacaggt gcttatatat ttgatacatg tgggtttcat ctatcatcaa tagttttagc
     7861 gaccgaaaat tactaataaa taagaatcat tgttctttat atgtgactga attattctga
     7921 attaattcct ggctttaagg ctccttttcc ttgtcacact gatggcagga ctcagaagtt
     7981 atatctgtat gcgttggttt ccctgagggt aaatcctatt gatttataca atctgtttgc
     8041 ctcggtcctg aaaggtcttg atgtcaggtt aacattttcg gctccctgct gcctggcaaa
     8101 ctcaattgca taattgatga tttctctgcc gaagcccttt ccccttaccg atttatcaat
     8161 taccacatcc tcaatccagt atttgtcagt cgtcggaata ggatatgtcg ccagagtaaa
     8221 catcccgata atcctgttcc ggtattcggc cacaaaaagg tgagtccctt tagttctgat
     8281 tattttcctg aaatgttctt ctgaaagtgg gggcgattcg ggtgaaagct gaggaatgag
     8341 atctgatacg gctttgaaag cctccctgct gtatcttttt atttccctga ttttcatcat
     8401 aatagaattt tagttttaat ggtatgatgc ctgccccgct caggcggggg aaccccttaa
     8461 aaatataaaa tcctcagaat atctgaaatt tagttagttg catttgtttt ccggtcaaac
     8521 ggatttctat attttcagac cgcatcaaca agttcgtgtt gatcaacaga gggaacttcc
     8581 agttgtgcat tcctgatcat aaagtgtaac cggttataaa tgttggcttc gctcattcca
     8641 ctgtcaaaat ccagaaacag cagattcata tttgggaaga gctcttttgt tcttttttct
     8701 attcctttgg aaataatatg attagctata catccaaagg gttgcagact gatcacattg
     8761 tttatcccat tgtgtgcaaa ttcagaaaac tctgccggaa ttctccaccc ttcgccaaac
     8821 tgagcattgg tgctgattat tcttgatgca tctcttgcac cttcatgaac gttggaaatg
     8881 gggaagtaat acggataatg gagaattccg gcttccattt tacgaattat tctgtaagcc
     8941 agattttcaa cgaaattcat tgcaaattta ggaatagtgc gctctttgac atttccctga
     9001 atccgggctt cctgacttgc gaatgtactg ataaaaaact gtatcagtgg aggcacgaca
     9061 gcttctacac cttgctttac aagccagtta acaacgtgct tatggccaaa gtcattgtat
     9121 tttacataaa tttctcccac tatgcctatt ctcgggatct ctttaagact gtttatactc
     9181 aggaaatcat cagctgcctg ctccgccaga tgaaagaagc ggttggcctc attattttta
     9241 agagcttcta tccccaagtt taaatacttt tctttcaggc gttttgcacc gccgatttcg
     9301 gtttcacggg gtgctgtgga atagtagaac tgcgaaatgc agtcagcata aacaattgca
     9361 cagaatattg cccggatgta tttttcccac ttcaccctaa aaccgggttg ttcattcaat
     9421 cctgctgtga aagacatagt taccacagga atgtcttcaa atcccgcagc tatcatagcc
     9481 ttttttatca aagccacata gtttgtagcg cggcattgcc cgccggtttg tgttattccc
     9541 aatgcaactt catttctgcc atacctgccg ctttccagag ctttcattaa atctcctact
     9601 accagagttg ccggatagca gatctcatta tttgagtatt ttaatccata ttccactgat
     9661 ttcttgtcgc ttggtggcag gttctccatt ttatagccca ttaattcaaa ggcggcaggg
     9721 ataaacggcg aataaaaatc tccaaaccac ggtccgagaa ttgttcttct cctgtcttct
     9781 ttttcgaaag gcggcgtatg tacagcaata tccttttcga aaacttcatc ttcttttttg
     9841 aacttcaggc tctcgataag cgaacgtatt cttaaccggg tagaaccgat attattaata
     9901 tcatctactt ttattatcgt tgcatttttg cctttgcggt gcaaaatatc aatcacttca
     9961 tccagaataa aggcatcagg accacatccg aatgatgtta tttgcacgta atgcacattt
    10021 tgaggtgctt cattcgctgt ccagagtgcc gatttgaaaa tccggttggt gtaagcccac
    10081 tgcattacgc tctgcacttc ttcggggttg gcttgcaggt ggcgcacaac atcttcagta
    10141 attacatcag caccgaaatc gcttaccaga tcggaaattt tgtgctgtat cagcggatca
    10201 atatggtatg gtcgtccggc aagcagtata accaggcggt tttcggcaac agccctctct
    10261 aatatctgat ttgagcgtct gttcaactgc tcttcatagc tctgctgagc aaccagggca
    10321 ttcctgaaag ctctctcaat tgtttttgta tctgttccgg gaataagggt tttgatatac
    10381 tccacacatg atttacgcat cagtttttta ttggcaaatg taaatgtcgg agcatcaaca
    10441 ggaatgccaa atttatgctt tggattgaca gcacttttaa ttacatcaga atagcctgta
    10501 ataatcggac aattgtagct gttactggtt ttgtcgtctt ctttgtcttc aaaaacgaca
    10561 tagggcataa aaatcctgtc cactttctta tctgccagat tatagatatg cccatgcaca
    10621 agttttgcag gaaagcagat attgtcagcc attaccgtgc tgatccccgt ttcgtacatt
    10681 gccatggttg atttatcgga aaggacgatc cggattccgc acccggtcag cagtgaatgc
    10741 cagaacggat agttttcata cattcccaga gtacgcggaa taccaagtgt aatttttcct
    10801 ttttccaaaa cggggcggtt aaacagcaaa tcatattttt cggtgtgcag atttgcccct
    10861 tttgctacgg tttctcctga attgttgaag accttttcgc atttattgcc agagtaaaac
    10921 acattgtcat tatcaaatct gaaatgagtt accgtacagt tattctcaca ccctttacaa
    10981 actgttcttt tttgtgaata ctctctcgat tctgccatgg cagccagcct gacaggcctg
    11041 gtttgacctg tttcaaactt tttgcgtgca tagagagctg ctccgtaagc tcccatcaat
    11101 tcgggataat cggtaaccat aatttccttt cccaattcta tctccaatgc acggataacc
    11161 gataaatttc taaatgtgcc gccctgcacc ataatatgac tgcccagctc tttgatatcc
    11221 ttcagtttca acaccttatt cagacagttt ttcactatgg aatagcccag tcctgctgaa
    11281 atatcctcta ctgaagctcc ttcccgctgt gattgcttca ctttggaatt cataaatacc
    11341 gtgcaacgtg tacctaaatc gcatgggtgt tttgatttca aggctatttt tgagaaatcg
    11401 gaaactgaat agttgagtga atcggcaaaa gtttcaataa aagagccaca gcctgaagaa
    11461 cacgcttcgt tgatttccac gcgtttaata actccgtttt caataaaggc ggctttcata
    11521 tcctgtccgc caatgtctaa aatgaaagag atgtctttgt tgaaatggca ggcgctcata
    11581 tagtgagcaa ttgtttcaac cattcctgaa tcaagatgaa aagccttttt gattaaatct
    11641 tcaccatacc cggttacaca acttccgagt acattcatct ctattccggc ttcctgtgtc
    11701 agcttgaaca gtttttgcag ccctacagtt acagcctgca gtgaaaatcc cttattctta
    11761 gtgtaatagt gataaaaaac cctggcattt tcgtcagtgg cgatgatttt tgttgtggtt
    11821 gaaccactgt cgatgccgat aaagcagttt ggatttttaa tctcctttat gtctatcgcg
    11881 gggaattgat actcttcttt ggattttagc cattcggtgt actcctgttt atctttgaaa
    11941 agaggtttta attgattatg agcatctttt ggattttctt tgacctcttc accaaaaagg
    12001 gaaagatatt cggagagtaa tctgctctct tgccggtcgc tcgcgatgat agctgcaccc
    12061 caggccgggg ttacttccgg attttcagaa atcacaactt ccgaatggtc catttttatt
    12121 tcatcgataa atacctttcg cagatatgga ataaatgtga atggaccacc acaaagaaag
    12181 actttcccct ccggctcgta tcctcttgcc agggatgtga ttacctgcat gcttactgcc
    12241 ctgaaaaccg atgcggctat atcagctttg cttgcgtttc gggcaagcag attctgtacg
    12301 tctgtttttg aaaaaacacc acaacgggaa gctatcggat agattgtttc ggcttgttca
    12361 gccaatgaat tgaactcatc catagtgaca ttgagcaatg ttgccatttg gtcaataaat
    12421 gagcctgtgc cgcctgcaca acttccgttc atccggatat caggcgtttt gccggatttg
    12481 aaaaaaatca ttttggagtc ttcaccgcca atatctacaa atgtctttat ttcagggaat
    12541 tgacgcaaga taacttcgca ggaagctact acttcctgga caaatgggac ttcataccgt
    12601 tcagctattc ccattccggc tgaaccggtt atacaaagtt tgacggggca atcgccaatt
    12661 ttatccagca tatcagataa aatctgtttc agattgccta aaacatcagc ataatgccgg
    12721 cggtaatctt tgtaaatgat tttattctga ggatcagaga ctactacctt gatggttgta
    12781 gagccggcat ccaatcctat gttgattaag ggttttatag ggttttgtgc tgaagaattt
    12841 atatgtgaga gtgatgagtt cataaagttt atctcgggca atagatgcgt ttttaaagca
    12901 attgattcag tttacagtct ggttcaggga tttcacactt aaaaacagga gtaaaacctg
    12961 tttcatattt tccagatgat tccggtgata ggcttctatt ttgcttttct cgttgagaaa
    13021 gaattcctgt ataaacgggt ctactaaata gggaacaaag agcaacccgt aaaaaagagt
    13081 aaccaattct tccattggca gttgtttgat actcttttct tctgcttcac ggtcgagcat
    13141 ggcatgaata agctgaaggt attcttttac tttctgattt gtattgataa aatcgaaaag
    13201 tgtttgcgga tgccgttgca tctccatcat aacaaagcgc ggcactgcat tgttacggct
    13261 taaaaaatca gcataaacgt caataatacc cagaagacgc tcctgaaaac tcagagtatc
    13321 tttgcttaac aggctctcaa agtgtggaat aatgccttct aaaatctctt caataatctt
    13381 gtaaaacaga ttctctttgg tgcgataata ataattcaag gctgttctgc taattccggc
    13441 cgtatctgca atgtcccgca tttttgcatt ttcaagcccc ttctccaaaa atacttgttt
    13501 cgcagcttca attattttca cctccgaatc gcttaactgg ctcattttat attatttaat
    13561 tgttctgacg ttagtgtttg acactaatgt caaaattagt ctgtttattt gaattgacca
    13621 aaaagaagac tgtctaaaag gagcagcccc gttttttggg gggatataac aattatttac
    13681 ttactgaagt tccgcaggca agaatgggtt gaaaagtggc acatagaaag ctttaggatt
    13741 tttaaatcct tctgaaaatc agtatactta aggtaattat aacaaaaacg acgtccgtgg
    13801 ggacgtcgtg aaagagggtg cccttttgag acaccctctt cgattatttt aagtcattat
    13861 tgatcagaca ttaagggtat agcggaggaa tccggttgct ctgaggctct tgtcatggct
    13921 ctcgaggtac tgccttatgt tgatcttgct ctccttgatg aattcctggt tcataagggt
    13981 gctctccttg aagaacttgt tcagtttgcc ctgggcaatc ttctcaatca tttcttcagg
    14041 tttgccttca cggcgtgcct gttcccttgc gatgtcaagc tcctgggcaa gtatcttctc
    14101 aggaacgtca tccttatcaa ctgaaacagg gttcatggct gcaacctgca tggccacatc
    14161 cttgtagacc tgcatgtcaa tacctgcctt gttgaaccct accagtgtgg caagcttgtt
    14221 accctggtgg atatatggct gtacggcttc tgctgttatt gtaccgaagt atgtaaggtc
    14281 aaatttttct ccggtgatac cgcttttctc aagtacgagg tcgctgactt ttttgccgtc
    14341 gatgacggta tttttgagag cttcaaggtc agccggggct gatttgaggg ctccgtcaag
    14401 gatcttgtat ccgagatcaa taaaatcagc gtttttggca acgaaatcag tctcgcagtt
    14461 cagtgagatc atcattcctg ttttgccgtc agctgttgtt ttggccagta caacgccttc
    14521 tgtggcttcg cggtcagcgc gcttgttggc gatagcctga cctctcttgc gtatcagttc
    14581 ctgggctttt tcaaaatctc ctgctgcctc ttcaagggcc ttcttgcagt ccatcatgcc
    14641 ggcgccagtc attcttctca gtttggcgac atcggcagca ctaatatttg acattttatt
    14701 taaatctgtt aattggttga tttgactatt cttcctcttc gatgctctct tcgacttcct
    14761 cttcttcgtc cttgtcagct gaatcctcta tgtcgtcgtc atccttcatt tcaggcttat
    14821 cctcctcata gtcggcagcc ctgaacttac ctttgcggga gccttccggc atctcttccc
    14881 ggtcagtctg tgaactctcc ttgtcctttt ctgccttgcg ctcattgagt ccttcagcga
    14941 tagcctggca catgatgtca accaccagtg aaattgactt ggcggcgtca tcatttgccg
    15001 gtatcacaaa atcgatatca gacgggtctg aattggtatc aaccattgca aacacgggta
    15061 taccgagtct ttttgcctca tgaacggcga taagttcctt gcagacgtca atcacgaaga
    15121 gtgcggaagg cagcctggtc atgtcggcta ttgaaccgag gttcttttcg agtttggctc
    15181 tctggcgggt gatctgcagc ttttccctct tggagaggtt gtcaaaggtg ccgtctgtag
    15241 ccatcttgtc aattgagctc atttttttga ccgccttacg gatggtgggg aagttggtaa
    15301 gcattccgcc cggccatctt tcggtcacgt atggcatatt aaccgctccc accttttcag
    15361 cgacgatttc cttcgcctgc tttttggttg ccacaaagag aaccttgcgg cctgatttgg
    15421 ctatctgctt gagtgcagca gcagcctctt caatcttgat caatgttttt tcaaggtcaa
    15481 tgatgtgaat gccgttccgc tccatgaaga tgtagggagc catcgccggg ttccattttc
    15541 tcttgaggtg tccgaagtgc acgccggcat ccagtaattc tttaaaattg gttcttgaca
    15601 ttttgatagt gttgttaagt ttacgttctg tgaagtcaat tgcagagtag ttcctggccg
    15661 gaagtcaatg caatttagat actaaacctg catctataat aattgctgtt gaactcctaa
    15721 cgcttgctga actggaatct cttgcgggcc ttgggtctgc cgggcttctt tctttcgacc
    15781 tcgcgcggat cacgcgtggt gaagccttca gccttaagtt tagacttgaa ctccggatca
    15841 accttgatca gagccctgga aatcccaaga cgaaccgcct ctgcctgacc ttttgatcca
    15901 ccgccgtcaa gggtgactgt gatgtcaaat ttgtcggcaa cattgagaag gttaagaggc
    15961 tggaggacaa catactgcag gatctcgttc tggaaatact gtttgtactc cctttcattg
    16021 atcatgatct gtcccttacc atctctgaga tacactctgg cgacagccgc tttccttctt
    16081 ccgatagcgt tgataacttc catgtttctt acttaatggt ttttaaatca atgggttttg
    16141 gtttctgagc ctcgtgtttg tgttcggggc ctacatagac gtaaaggttc ttgaagatgg
    16201 cggcgccaag cctgtttttc ggaagcatac ccttgacggc atattcgatc agccgtgcag
    16261 gattcttctt catcagagca tcagccctga caattctctg gcctccggga taaccagtgt
    16321 gcctgatgta ctccttgtca gtcatcttcg ctcccgtcag cgcaattttt tctgcattga
    16381 taacaattac gttatcacca cagtcaacat gaggcgtaaa gcttgtcttg tgcttccccc
    16441 tcagcatcag ggcaacaaca gatgccagac gcccgagtgc ctgaccctcc gcatctacaa
    16501 gtacccactc tttctcaaca gtcgccttat tggctgaaac tgtcttgtag cttaaggtgt
    16561 tcactttgta cttaaggttt tgttaattaa tctgttttta aaataaaaac cccatttttg
    16621 gggtctgcaa aaatacaata tatttttatt tcgagcaata tgttgtactt ttaatttctt
    16681 taatatccgc atgttcagca gaaacatcca tcagaccgta catctgatct gcctcctgtt
    16741 ggccgcgttc atgatgcctc tctccgtctg gctcctctcc gtggtatcta ttatcatgtc
    16801 tgtcaactgg ttgctcggag gcgaataccg gcacaagatc aatattttaa gatcacgccc
    16861 gggtgtcttg atactgtccg gactcttcgc gatgtacctg gtgtggctgc tgaatacaac
    16921 cgacttccat ggagcagttg ctgagctcag gcttaagctt ccgctgcttt tcttccctgt
    16981 tgtggccgga tcaaccgaaa gtgcagagag gtcacttctc cggatggttc tgctttcctt
    17041 catcgcagga tgcctgtttg ccactgttgc cggcttcgcc gcactcgccg gactgacgcc
    17101 cggagagata cactcttccc gcgaacttgc actgtttgta ccgtcaatac ggctctccat
    17161 attgctctgc tttgcgattt tttcctcttt atggcttatc atttatgaca gtgcggggca
    17221 gggaaaggca gggtctcatt tgccagagtg gtggcagcgt ctgaatagcc ggctccttcc
    17281 cggcaccggc gggaggctgt acgtggtgat gctcgcggcc gcagccatga gctatttcct
    17341 gttcaggctt ctctcggtga cggggcttgt tatttttgtg ctgctgctct tcctgacagc
    17401 tgttttnnnn nnnnnncggg ggcaggcgcg tacgttgggg cataaccttc ctggctgtgg
    17461 ccgcgacgat aaccgctgca ttgacaatcg tgtcactcag cgcatggaac agacttcata
    17521 accctgccga tccgtcggtg aacgggaagc ggcagctcac attgtcgggc agggcttata
    17581 cccactatcc tgaggagacc cttatagaga acggctacct ggtctgggta aatgtgtgcg
    17641 aggaggaatt gcggcaggag tggaaccgga ggagcctgaa accatacgat ggcgctgatg
    17701 cagcaggcaa tgagctgcgt gtgaccctgg tgagatacat caccgcgaag ggaatggcca
    17761 aggactcggc agcagttgca gcgcttacag acagggacat tttcaatatt gaacaggggt
    17821 atgccaatcc tctttatgcg aggaagggga gccccatggc cagggcgtat gagattgcct
    17881 gggaacttga ccgtgcagcc agcggagcca atccctcagg ccactcactc acgcagcggc
    17941 cggaattcta ccgggcagcg gccggaataa tcaggaaaca tccctggaca ggagtgggaa
    18001 caggtgatct ggcccgcgcc ttcagtgatg agtatatatc aatctccacc ccgttggagc
    18061 ctgaatatcg cctgagggcg cataatcagt atctgacctt cggagttacc ttcggcattc
    18121 ccgggatgat actggctatt accctgctgc tcataccgtg gctgataaac gacggccgct
    18181 tctcatacct gtttctggta ttcatgacac tcatcctgat ctcaatgttt aatgatgata
    18241 cattcagttc atttaccgga gcgtcattct tttcttattt ttacaccctg ttaattgtca
    18301 cttcagccgg taaggagtat attcatcata gccggcagtg cggggaagaa tgttaattgt
    18361 cacatttgac ggtaatgagg ctcaatatca aggacaggaa atatcttgaa cttgcaataa
    18421 aaactgcagg ggagaatatg gaagatggcg gagggccatt tggtgcaata atcacaaaag
    18481 gcgaaaccat ccttgcccgg gcaggcaaca gggttgttcc cggacatgat ccgacagccc
    18541 atgccgaggt gatagccata agaatggctg cagagaagct gggcacgcat gacctgtctg
    18601 actgcaccat ttacgcttca tgtgaaccct gtccaatgtg tctgggagcc atttactggg
    18661 cagggatcag gcgcctggta tactcctccg acaggtacac cgcagcctcc gcagggttca
    18721 gtgacaatca tatttatgaa gagctggcgc ttgacaatga cagcagaagc attgagatgg
    18781 tccgcggact gaagaaggag ggagacaacg tactgaaaag gtgggtggat ctccctgaca
    18841 agatacagta ctgaacagca gcaatgggta aagtcgacag ggatttctat cttgcagatg
    18901 cagttaccgt cgcccggcag ttgccgggta tggagctggt catggcatcc ggagagcaat
    18961 gtctcagata tgtaattacg gagacggagg cctacctggg tgagggtgac agggcatgcc
    19021 atgccagcag gggaagaacc cgcaggacag aaccgatgtt cctcacagga gggcatcttt
    19081 acctctattt cgtctacggg atgcactgga tgctgaatgt ggttaccggc cccgctgatc
    19141 atccacaggc tgtgctcata aggggggtgt cacaatacca ggggcccggg cgcgtcacaa
    19201 agggacttgg gattgatggt tcattcaaca gggaggacct gacatcgtca ggcaggatat
    19261 ggatagagga taccggcttc cgcccggagt tgatctcagg gccgagggtg ggaatagact
    19321 atgccggcga gccatggata agcaaaccgt ggcgcttcat tgtaagaaac gaaaaagaaa
    19381 agatatgaaa aagggattca gactatggaa cagcataacg ggctgggcag tgtttgccgt
    19441 ctcgcttgtc atctatctga tgaccatcga acccactgtc agtttctggg actgcgggga
    19501 gtttatcctc tgctcctaca ggctcgaggt ggggcatccg cccggagctc cgttcttcat
    19561 gctccttggg cgcttcttta ccctgtttgc cggaggcgat acctcgaagg tggccatgat
    19621 ggtaaacata ctttccgctt tggcgagcgc ctttacaatc ctgttcctgt tctggaccat
    19681 cacaaggctt gtccgcctcg ccaccggtga tgacgaaacc gtaaccggag gaaaatcact
    19741 ggccattctg gctgccggag caatcggtgc actggcatac gctttctcgg acactttctg
    19801 gttctcggca gtggagggtg aagtatatgc caccagctca ctcttcactg ctgcagtggt
    19861 atgggcgatg cttcgctggt atgatgaagc cgacgatccc cgttcaggga gatggatcat
    19921 tctgatagcc tatctcatgg gcctttcaat aggggtgcat ctgctgaacc tgcttgcact
    19981 gccggtgata gtcctgatat ggtacttcag gaagcacaag gcaacgactc gtggcttcat
    20041 ctatgcactt cttgccggtt ttgttatcct cggtgtgctc aacttcgtgt ttgtgccggg
    20101 cataccaaca gtggccggat ggtttgagct cttctttgta aacaccattg gtctgtctta
    20161 caatacgggc ctttatatct atctgatcat cctgaccggc ctgatagcgg gagggatatg
    20221 gtattcactg aagaaggggc gcaggatact gaactacgtg atgacggtga ttgccgtcct
    20281 ggtgctgggt tattcatcgt tcgccatgat aatcataagg gccaacgccc acccgcccat
    20341 gaaccagaat gacccgtcag atgtcttttc attcatctat tacatcaaca ggtcgcagta
    20401 cccgcgtgct ccgttcctct acggcaacta ttactcagcc ccggtgattg gacttgagaa
    20461 gaagatcggc ggttacaaca agaaggatgg aaggtatgag ccttattata ctaccgaata
    20521 caagtatgac aagcgtttca tgaccatttt cccgcgcatg tacagtactg atcagggtca
    20581 tatccgggag tatgagtact ggggcaaggt aaggggaaaa aagatcactg tcagatcggg
    20641 ccagggcgat gagcaggtaa ccctacccac ctttggggag aacttgcggt tctttttcag
    20701 gtaccaggtg ggagtgatgt acatgaggta tttcatgtgg aacttctcgg ggcgccagaa
    20761 tgacatccag ggcaacggca acgtcatgga cgggaactgg atcaccggca ttcccttcct
    20821 tgacaatccg cgtctcggtg accagtcatt gcttcccgac gactaccgta acaacccggc
    20881 ccggaatgct tatttcctgc ttccgctgct tgccgggctg gccggcctct actggcagta
    20941 caggcgtgac ggcaaggggc tgtcactcgt gttacttctt tttctcatga cggggcttgc
    21001 cattgtgaca tatcttaacc agaacccgag tcagccccgc gagagagatt atgcctatgc
    21061 aggttcattt tacgcctttg ccatctggat aggaatgagc gtcatgctct tctatgatct
    21121 tttgaggaag acaatgaagg atcatcctgc agccattctc ccgtcggtgc tgctgctggc
    21181 ggcggttccg ctgctgatgc ttgtccgcaa ttatgatgac catgacaggt ctgacagata
    21241 tactgccagg gatattgcat cgaattatct tgctacggtg gatgagaatg ccgtgctgct
    21301 tacctatggt gacaatgact cgttcccgct gtggtatatt caggatgttg agggcttccg
    21361 cactgatgtg cgtgttgcca acctcagtta cctgcaaagc ggtttctata tcgaaacaat
    21421 gagccgcaag gcctttgaat ctgatgcact gccgttcacc attccggttg aaaagtacat
    21481 tgagggtgcg cgtgtgcagc ttcccgtaag ggagctaata aaggaaccca cacctatccg
    21541 taaggtaatg gagtttgtcg gatcggatga tccgaaggcc atggtagatc tttcgggccg
    21601 gggtgattac ttcaatttta tcccggtcac ggaattcatt gttgacgttg actcggcaca
    21661 tgtaattgca acgggagccg tgcgtccgta tc
//
LOCUS       DAOPBK010000017        21295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2608_length_21295_cov_5.075471, whole genome shotgun sequence.
ACCESSION   DAOPBK010000017 DAOPBK010000000
VERSION     DAOPBK010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_21295_cov_5.075471"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2664)
                     /locus_tag="PLA17_01930"
     CDS             complement(<1..2664)
                     /locus_tag="PLA17_01930"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPK84201.1"
                     /translation="MRKYILSFLLSLLCSGLCAQVELKEPVDYVDPLMGTTIPQWTLF
                     PGATTPSGMVKISPDNQVNGWEAGYDYKIESIAGFSHIHSRTMGGLLVMPVTGELKTT
                     PGKPTEPGSGYRSRFSHSNETALPGYYSVFLDDYGIRAELTSTTRTAFQRYTFPEADT
                     ARIVIDLQIPTDRGYQILEARVRKVSNLEIEGFSTQQSLLGAENNLYTIHFVIRFSKD
                     FVSFNGWVGETILRDRGDIHQLYDNEDAGLFLNFRTTEGEEILVQSAISLVSIEQARL
                     NLDTELGPFAWNFNSVKEKSREEWNTLLKTITVEGGDEENLRMFYTSMYRAFAGHTTW
                     SDVNGKYVDMNEKVQVLTDPAYPVYGCDPAGTGTIALFQLRNLCDPDRANKCVRSLLE
                     LYARGGWIPEASEGIEYNATMASSYAIAMMGAAFQQGIRDYDTSMMYKAIVHDRTVPG
                     VPHAGGGFAGAGYLDSYIKSGSVTGVTDSAAATSEYAFIDFATSQMAKVSGNISDYKT
                     FTKRAFSYGNLTCHDNRYLSVFAPHDVSGIINTFGRETFIDRLEKSIEGSLRHGEPGK
                     ITPDMIRQIRYSGQYNIEQSPLQAAWLFNYAGVPWLTQKWTREIMSGYSSGDACIITP
                     GLDPAQAGALFVMSAIGLFQTNGGTSAEPFYEIGSPLFKKATIHLDGRFYPGGEFIIL
                     AENTTDKNRYIRGATLDGTVLDKPWFSQSELAKGGTLLLTMGSRPNHKWGSDPDDAPP
                     SMSTRLTPKETDAIMKYDKYAEDLAAWNQALRAYYYHKKEHFESLPNTENEIIFLGNS
                     ITDNSEWWELFSDTHVKNRGIGGDDTDGILERLDEVTESSPSKIFIMIGTNDLSNGKS
                     VEYIVENYRKIIACIKHSTPDTKIYIQSILPT"
     gene            complement(2751..4085)
                     /locus_tag="PLA17_01935"
     CDS             complement(2751..4085)
                     /locus_tag="PLA17_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPK84202.1"
                     /translation="MMPQCLSAQTVIWEAPEHFNKFNSVTGGWLASDATISLPLPDDK
                     TLWLFGDSFIGEKNGEFGINPLKSKFINNSAILETGDDLTSLYGGTFENPSSFVPGEG
                     ADYFWPEHAVIENDIVKVFAIRIKNVDYGTPGFNFQVGTTYKASFKYPGFEHISTTRF
                     ESVTDSTFRFGACVIKSGQHTYIFGVKDTTAGIFTYPLPYLARVSESIEEPWEFYAGS
                     DTWSNDLADAVSIGNRGMSESFYVYEKNGKFYLIMHEIWLVGELWILEADKITGPWNT
                     KASGGIENRFAVIRKPAVNLTYNLFAHPHFQNDGRILVSFNVNTSNFASIYTDTRNYR
                     GRFYWLDVEGAVVASVPDTIKLFSTPVGIGSVPAGYDQGRISYHPATRSIHIRNVNTP
                     SLLSIYGTDGRLYLSRSIEMDTEIDCSHLPAAIMIISLRSGGAIETKKVFLK"
     gene            complement(4185..6521)
                     /locus_tag="PLA17_01940"
     CDS             complement(4185..6521)
                     /locus_tag="PLA17_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84203.1"
                     /translation="MKKSIFILLLPALIGFAGCNDSKSVQTGLSHAIPSLEELSGVWV
                     STDTVVMEPSIRNFRGQALLNRDMTSISWFVSAPFSGGYHTGTLRVNGKTPGANSWRW
                     QPYQALRKGSIDNLDFLSSTRMLFEKDAVMWEVEITNSDTVTREVNLELDLIGFISKF
                     GGDWQWWYPYPSMSGTVTNRNEEVENIREHLGENVFEKEGWAWELVDGKPVLQKVKMR
                     WPGDADIMNSKKFTTNVENNTVYIRDTESQAITSFSVVTGPDRLTGSNCGATASWKYE
                     LKPGEKRTIKYLMAYGDNPETIKQNVTDWSAGFDTLFSGIKGSWEEKWDEIFKPGNSL
                     ISGCFPILVTDDAVARKVYYTSPLTMLYLINTNLPEHSKVFLTGGPRWGASITFFWDI
                     TLWSTLWAVVDPVMMKEHLSSWIKIDPSKYYGKDNFGGKGVGNGYSANYWALFQLIRD
                     YVSVSGDYAYLDETIGDKTVLQHLDDYSMNWKNISLYGTEGCTDDVYRLADFGDDEWN
                     LLECVPTYKHIVPSFNVGYAWMMRETAKLHQHRGEQQKADSLNVLASEMVKRILQLYA
                     GNGVWNSLYPGNKKIEVRHCLDFSFFGKYIPDDIPANIKSEMLGFLYDELMTDHWMRA
                     QSLKDLAADKSDRADHGPFGAFDGWPVDIMDALTQMGFPQQALDFYHAVEPVTYEGIW
                     AQAHELWGENKTNKKALVRIPERDWCNRESSSGVAFAQTMLKDFFGFYPDPDGTAIKA
                     SQGFQFEGSMHHVYYQNEYYTIKYRSGKPVMIKEKTRK"
     gene            complement(6534..8318)
                     /locus_tag="PLA17_01945"
     CDS             complement(6534..8318)
                     /locus_tag="PLA17_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HPK84204.1"
                     /translation="MKDTINENEILKPNFNKVPVIRVAEFNEGYFKGWDSIFGVLSGR
                     IKSVSGQKTVIVVDCNLGIYYDELLSIFSERLEPELVITSNDYLFSPEEVQRITWPDV
                     TNDRIFGYLTRFNFSDLTDTAKLNSCREKVRDLTEGLVLVFGYGASLIADEPLILVYA
                     DMPRRETELRMRAGIVGNLGLRNRQELAETKYKRAFFVDWRVCDRHKKKLMARWDFVL
                     DTTDMDDPRMITASAFSRSLRKALTTPFSIVPTFDQGIWGGQWMKKMFALDPEPENYS
                     WCFNCIPEEQSLMLDFGEGLFEMPSVNLVFAHPAELLGDAVYGRFGDEFPIRFDYLDT
                     MDGGNLSLQVHPVTEYIQEKFGMHYTQDESYYILDTGEDPSVYLGLREGINPREMIDD
                     LNKAQNDGSMFDDVRYINRWPAKKHDHFLIPGGTVHASGKNCLVLEISATTYIFTFKL
                     WDWGRVDIDGRPRPINIDHGMNVIQWYRTTDWVRKNLINRFRKISEGDGWTEESTGLH
                     EREFIETRRHWFSKKVNHNTNGGVNVLNLVEGREIIIESPDNSFEPFIIHYAETFIVP
                     ASVGAYSIRPYGESEGKLCATIKAYVRT"
     gene            complement(8302..9768)
                     /locus_tag="PLA17_01950"
     CDS             complement(8302..9768)
                     /locus_tag="PLA17_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK84205.1"
                     /translation="MKKIIIFLTFLSITFGSCQDFLEQDLRSELNYDNYFSSEKDLVS
                     FADGMFGGLITWTWTGGGLFFNNYWVLQDLASDNCYETGPSIDMLDLAQFTFDVNNAT
                     VYYIWVGCYEVLNSTNVLLEESENIKDYSRPEIKNHLQGEAYFLRGMLYFDLVRLFGD
                     VPLQLFSTKTAAETQIARTPVADVYESIISDLEKAEQLLAVNPFPNRVAGMPTSMTAS
                     ALLAKVYLTRGALNNNAADFNSARTYLEKVLGHYTLEPEYADIFKLSNANSGEILWAI
                     NFSGTLGEGWTTNQFIVRLMPTTISENGTRNGQGWERPLDILYDSFSDTDKRKAATFI
                     TEFEGETFDGPFIRKYWDQEAEGGRQNGESDVDYIYLRYSDVLLMYAEALNEIHKGPT
                     TEAYDAINAIRERANLDDLAAGLSYQEFKDALLQERQWEFVMEGHRWFDLVRMGKLLE
                     RVQIAKPTANIQTYHLLFPIPQKERYLNHKLTQNEGYN"
     gene            complement(9786..12869)
                     /locus_tag="PLA17_01955"
     CDS             complement(9786..12869)
                     /locus_tag="PLA17_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK84206.1"
                     /translation="MKYSILFLAFILFASTAFTQEVTIRGKVVDEDKAPMPSVAVVIE
                     GTTTGTMTDADGMFTLAIDKGTARTITCSFVGFSKNSTRIVEGVTYYEFTMVSETTEL
                     DAIVVIGYGTQRKEDVTVSISTVKAREIDSPAITSLDQSLQGQASGVVVSQSSGKPGA
                     PVSIRIRGTTSINGTNEPLYVIDGIPIITDPSELTTGTIQGSAINPLSSINPQDIESM
                     QILKDASATAIYGARGANGVILINTKRGKKGALQVNLNFSYGMQQLAKKLDLLNAREL
                     AELGNDAVTEARKYYPQIAYGSNFAVPSRFGEGTDWQGEIFRIAPLSNSQISLQGGNE
                     STVYFLSANLVTSKGIIHNSDFNKGTVRVNLDTELSEKVKTGVNLNLSQSVSNGVTTG
                     VPNVASSVTAMALLFNPGQEVNDSTQVGGYTYESNTINAIPNPVAEMNETDIVITTDR
                     MIGDFYIDWDIIKNLQFKFKAGVDAFFNKEQQYIPSYIKRGQDKGKGSNVNIQGYTWL
                     IENTLTYKFAIDKHHFNVLAGQTAQKFVSDRAEIAVERFEDERLTYYNLGMGLDKTIM
                     SGYSSWAMLSGLGRVMYNFDSKYYMTVSGRLDGSSKFGTDNKWGFFPSVSAAWRISKE
                     NFLANAKAINDLKLRLSAGSVGNEGIPSGATLSLMGTHQYFYGEGPGAEVIGTYVYSL
                     QNNDLKWEVTRQYDAGIDLSLFRSRIEFTIEAYLKNTSDLLLYLPVNRSSGFEYVWAN
                     VGDLQNRGIEFSVNSVNFEGDFRWNTRFNLSFNRNEVTNLDKSDNIYGTSVMNILDWT
                     MISEGQPIGVIYGYKSDGIIQLDEDPAKVPFFPSKIARWGDRKYLDKVPDGKLTVDDY
                     YRLGNVNPDFSFGLRNTFSYRNFSLDIYIQGDYGNEIVNFNRIQLESFDGYQNNSAVA
                     LERWTETNPTNEYPRANASPHGIIMSDVWVEDGSYARLKQVIFSYNLPKELLSKIKIS
                     NLQLSLTGYNLYTLTRYSGYDPEVSIFGGSVFGKGADYGAYPTARSLLFSINATF"
     gene            complement(13050..13925)
                     /locus_tag="PLA17_01960"
     CDS             complement(13050..13925)
                     /locus_tag="PLA17_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84207.1"
                     /translation="MKKRILLLSAIALMVIGLTVFLVSCKKDIVTEMTISQTATPQLN
                     IGDNATAVITIKADGVKSFKVYKLVDNVRDEGTDVKADLIKSENTYTYNFSYTVQEFD
                     DLHTLGFEFEMVDDEDLQKKVGLVVNINISLKSTFVKYDWKVTASTWMGVDVLTAADA
                     AIVYRFSADGTYQEDLGADYASDFHHFCYWVFKETPANGDTIAIVRLVRRLKQGDTAI
                     DEYYDYNITAASETEMTMYWDLEVWGLLDIENKFKSQPKGAFVPYGTAEMEAIVNANA
                     SLSCSNISQSLLTIQ"
     gene            complement(14082..14816)
                     /locus_tag="PLA17_01965"
     CDS             complement(14082..14816)
                     /locus_tag="PLA17_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPK84208.1"
                     /translation="MNFKIDHKSSSPLHIQVENLLREMIRDPEYSKGKFLPREVDLAK
                     LLGISRNTLRQATNKLVYEGLLIRKKGVGTIVANTAVHTKLKNWLSFTQEMKTLGIDI
                     INYELKVTWIEPEQEHIGFFRIPEKKTILRLERLRGSAEGPFVYSVSFFHPRIGLTGK
                     EDFNQPLYEILEKEHATVAKLSREEISAVAADGSLARMLNLHTGDPILKRKRFVYDPG
                     SRPIEYNVSFYRTDSFVYSVESEREL"
     gene            15038..16276
                     /locus_tag="PLA17_01970"
     CDS             15038..16276
                     /locus_tag="PLA17_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK84209.1"
                     /translation="MSSRDRIIAAINHRQPDRVPVDLGASTVTGISAIAYNNLKKQLG
                     INQPARVFDVVQQLANIDMEVINMFGVDALDINRISAESGEWYEVELADGSRAEFPHW
                     YQPVKMPDGSWQTFHDDGTVLSRMPVGATFFDQMFFPYENGYPDSFDNLNDALTKISW
                     VVHSHSSNLNDEELREKLIKLKELSGKALVMSGGVKLLELGFFIRRMDNFLTDLLLEP
                     EKLSQMLDKFVEMHLAGLENKCDSLGDIVDVIRFGDDLGMKTGPLMDLDTFRRFFKPR
                     YKILCDYVKKHTGMKIFLHSCGSIRQFIPDLIEVGIDILNPVQTNSHDMDPASLKKEF
                     GKDIVFWGGGVDTVSVINHGTPEEVRKDVLKRCEIFSKDGGFVFAPIHNILSEVPPMN
                     IIAAYNAVREFNGQPNCIND"
     gene            16906..18213
                     /locus_tag="PLA17_01975"
     CDS             16906..18213
                     /locus_tag="PLA17_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPK84210.1"
                     /translation="MTKNNNAPLSDRFNIGQDWASVVTGFILILFVVITGYAVATPSF
                     GGKAGWNFDHDFTGMFGASSLWTSLLFTLMISVVTATIGMLLSGEKIRNYFTGFVAIF
                     LLVILAQFVSSFAGFKNLGLETVLFSLIIGLLIGNFIRLPEWLRSGVQTELYVKIGLV
                     LLGATILFRDILTAGAFGLIQAVIVVTVVWYFAFWLSRKMKVDDEFSTMLASAVSICG
                     VSAAIATAGAINGDKKKLSFIISLVLIIAIPMMIFLPVIAKWMGLSEVVTGAWLGGTI
                     DTTGAVVAAGTIAGETGLKYATIVKFSQNVLLGLAAFAISIFWAYRSRGAGEKGQHVP
                     VKIIWDRFPKFVLGFILASLIFSFILSPGMAESSGKVIKSFSTFWFNLAFISIGLETR
                     FADFKQMESRKPFYSFLIAQTFNIIFTLGVSCLLFQLINTNIK"
     gene            18210..18794
                     /locus_tag="PLA17_01980"
     CDS             18210..18794
                     /locus_tag="PLA17_01980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK84211.1"
                     /translation="MNKVRNIIFLLITSAIMALSSCDGKKEVIINTGFGEIHVALDLQ
                     NAPVTSANFLRYVDAGLFDSACFYRVVRPDNQQNDSIRIAVIQGGRYNQEETDGFPPI
                     AHETTEMTGIRHLNGTISMARWTPGTATSEFFICVGDQPELDYGAKRNPDGQGFAAFG
                     QVTKGMDVVMKIHSIEAPAQYLDKPILIYSIKRK"
     assembly_gap    18808..18907
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19053..19898
                     /gene="nfo"
                     /locus_tag="PLA17_01985"
     CDS             19053..19898
                     /gene="nfo"
                     /locus_tag="PLA17_01985"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPK84212.1"
                     /translation="MKYVGAHVSASGGVENAPLNAYNIGAKAFAFFTKNQRQWVAPPL
                     TSKSIEGFRKRCEEYDFGPHQILPHDSYLINLGNPDPEGLGRSRHSFIDEMKRCELLG
                     LDRLNFHPGSHLGKMTEEECLDLIAESVNIALDKTAGVIAVIENTAGQGTNLGYRFEH
                     LRHIIDRVDDKSRVGVCIDTCHAFTSGYDLSTEEGFEKVFNEFERVVGFNYLKGMHLN
                     DSKKPFGSRVDRHDNLGKGLLGNEVFRLIMNDPRFDGMPLILETPDENLWVEEIKYLY
                     SLQNR"
     gene            20061..>21295
                     /locus_tag="PLA17_01990"
     CDS             20061..>21295
                     /locus_tag="PLA17_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPK84213.1"
                     /translation="MTNYIILALCVIIILSYLFDISSRYSRVPGIILLIGLGIALQLI
                     SLSTGFDIPNLKPILPVIGTLGLVMIVLDASLDLKLEKKKSRVLINTVLSALVLLILV
                     VTIGSLVLVKIFGHTPISAVLNMIPLGIISSAAAIPSAEGLSSKEKEFIVYESSFSDI
                     LGIMAFDFILLGQSEMNLGVGNFLIAGFLTIVIAAVTTVGLAWLLHKANYHVNYVIIM
                     TFVVLVYVLAELYHLPALLLVLVFGLALSNNRIVENTFISRYIEFGKFNRDLNAFRRI
                     LGELTFLVRSFFFIIFGYYTSVEGVFYPENLITAFIITAGILLIRWLFFYCILRIKSL
                     PLVLFAPRGLITILLFLSIPAAYMIPEINTEVITLVILMTLLAMMLGNIISPRKAKRV
                     VSAAKTDPQVKNVLGVFDYA"
BASE COUNT         5431 a         5069 c         4698 g         5997 t
ORIGIN      
        1 agtagggagg atactctgaa tatagatctt tgtatctggc gttgagtgtt tgatgcatgc
       61 tattatcttc ctgtagtttt cgactatata ctccacactc ttaccattag aaagatcgtt
      121 ggtcccgatc atgataaaga ttttcgacgg actggattca gtaacttcat caagacgttc
      181 caggataccg tcagtgtcat ctccgccaat acctctgttc ttgacgtgtg tgtcactgaa
      241 gagctcccac cattcgctat tgtcagttat gctgttacct agaaaaatga tttcattttc
      301 cgtatttgga aggctttcaa agtgctcttt cttgtgatag taataagccc tgagcgcctg
      361 gttccaggct gcaaggtctt cggcatactt atcatacttc atgatcgcat ctgtttcttt
      421 gggcgtcaga cgggtgctca ttgacggggg tgcgtcatca ggatcactgc cccacttgtg
      481 gtttggccgc gatcccattg tcagtaacag ggtacctcct ttagccaatt ccgactggct
      541 gaaccagggc ttgtcaagga cagtgccgtc aagtgtggcg cccctgatat acctgttttt
      601 atcagttgta ttttctgcca ggataatgaa ttctccccct ggatagaatc ttccatcgag
      661 atggattgtt gccttcttaa aaagtggact gccaatttca taaaaaggtt cggctgaagt
      721 gccgccattt gtctggaaaa gcccgatggc gctcatcacg aataacgcac ctgcctgtgc
      781 aggatcaagc cccggggtga tgatacaggc gtctcctgag gaatatccgg acatgatttc
      841 acgtgtccac ttttgtgtca gccatggcac acctgcatag ttaaagagcc atgctgcctg
      901 tagtggggat tgttcgatgt tgtattgacc agaatatcgg atctgcctga tcatatcggg
      961 ggtgattttc cctggctcac catgccggag tgatccttca atgctttttt ccagcctgtc
     1021 aatgaatgtt tcccgcccga aggtattaat tattccgctg acatcatgtg gagcaaaaac
     1081 ggataaatac ctgttgtcgt gacaggttaa attaccatag ctgaatgctc ttttcgtaaa
     1141 tgttttataa tctgaaatat tacctgatac ctttgccatc tgggaggtgg caaaatcgat
     1201 gaaagcatat tctgatgtag cagcagccga gtcggtcact ccggtcacgc ttcccgattt
     1261 gatataagaa tcgaggtacc ctgctcctgc aaatcctccc cctgcatgag gcacccctgg
     1321 tactgtcctg tcgtgaacga ttgctttgta catcatgctt gtatcataat cgcgtatacc
     1381 ctgctggaaa gcggctccca tcatggcaat cgcgtatgac gaggccattg ttgcattata
     1441 ttcaatgcct tccgaagctt cggggatcca tccgcctctg gcatatagtt ccagcaatga
     1501 tctcacacat ttgtttgccc ggtcaggatc gcacagattc ctgagctgga aaagagctat
     1561 ggtacctgtt cctgcaggat cacacccgta aacagggtaa gccggatcgg tcaatacctg
     1621 taccttttca ttcatgtcaa catacttccc gttcacatca ctccaggttg tatgtccggc
     1681 gaaagcacga tacatgcttg tatagaacat cctgagattc tcctcgtcac ctccttcgac
     1741 ggttatggtt tttaaaaggg tgttccattc ctccctgctc ttttccttca cagaattgaa
     1801 gttccacgcg aatggcccca gttcagtatc caggttaagc ctggcctgct caatgcttac
     1861 gagcgaaatc gctgactgaa caagtatttc ttctccttcc gtggtcctga agttcaggaa
     1921 gagacccgca tcttcattat catataactg atggatatcg cctctgtcac gcagtattgt
     1981 ttcccccacc catccgttga atgatacaaa atccttgctg aatcgtatta caaaatgaat
     2041 tgtgtacagg ttgttctcag ctcccagcag gctttgctgt gttgaaaaac cttctatttc
     2101 caagttgctt acttttctta cgcgggcctc aagtatttga taaccgcggt cagtcggtat
     2161 ctgcagatct atcacgattc gggctgtatc agcttcagga aatgtatatc tctggaatgc
     2221 tgtccttgtc gttgaggtaa gctctgccct gataccataa tcatcgagaa agaccgagta
     2281 ataaccaggc agcgcggtct cattactatg gctgaagcgt gaacggtaac cggagccggg
     2341 ctccgtgggt ttgccgggtg tcgtcttcag ttcgccggtg acgggcatga ccaggagtcc
     2401 gcccatggtc ctggaatgga tatgactgaa gcccgctatg ctctcaattt tgtaatcgta
     2461 tcctgcctcc catccgttta cctgattgtc aggactgatc tttaccatac ctgaaggtgt
     2521 ggttgcgccc ggaaaaagtg tccactgtgg aatagtagtg cccatcagcg gatcaacata
     2581 gtcaactggt tctttcaatt cgacctgtgc acagagtccg gaacagagta aagacaaaag
     2641 aaaggaaaga atatactttc tcataagcgg tgatttgtat gggctttctc tgaatagcat
     2701 agaatagaag tattttattc cgggttcata aacccgataa ttctgcctgt ttatttcaga
     2761 aatactttct ttgtttcaat agcaccccca cttctcagac ttatgatcat tattgcagca
     2821 gggaggtggc tgcagtctat ctctgtgtcc atttcaatac tgcgcgacag gtataacctg
     2881 ccgtcagtgc cgtagatgct cagcagcgat ggggtattga cattccgaat gtgaatgctc
     2941 ctggttgccg gatggtaaga gatccttccc tgatcgtagc ctgccggtac ggagcctatt
     3001 ccaaccggag tgctgaacag tttaatggta tcaggcacgg atgcgacaac cgcgccctca
     3061 acgtccagcc agtagaaccg gcccctgtaa ttacgggtgt cagtgtatat ggaagcgaag
     3121 ttggaagtgt tgacattgaa actgacgagt atcctcccgt cattctgaaa atgaggatgg
     3181 gcaaagaggt tatacgtcaa attgacagct ggcttcctga taactgcaaa ccggttttca
     3241 atgccacctg aggctttggt gttccaaggt ccggtgatct tatcggcttc aagtatccac
     3301 agctctccca ccagccatat ttcatgcata atcaggtaaa acttcccgtt cttttcatag
     3361 acatagaatg actcgctcat cccacggttg cctatggaga cagcatcagc aagatcattt
     3421 gaccatgtgt cagacccggc ataaaactcc catggttcct caatggattc tgacaccctg
     3481 gcaagatagg gcaagggata ggtgaaaatg cctgcggtgg tatcttttac cccaaagata
     3541 tacgtatgct gaccactctt gatgacacac gctccaaacc ggaaggtaga gtctgtcacg
     3601 gactcgaacc tggtggttga tatgtgctca aagccaggat acttgaaaga ggccttatat
     3661 gtcgttccga cctgaaagtt aaatcccggt gtaccgtaat ctacattctt tatcctgatg
     3721 gcaaagacct ttactatgtc attctcaata acagcgtgtt caggccagaa gtaatccgct
     3781 ccttctcccg gaacgaatga ggaagggttt tcaaaagtgc cgccatacaa ggaggtcagg
     3841 tcatctccgg tctcaagaat agcagagtta ttgatgaatt tcgatttcag tggattaata
     3901 ccgaattctc catttttttc tccgataaag ctgtcaccga acagccagag ggttttatca
     3961 tctggcaggg gaagggaaat agtagcatca gaggcaagcc atcctcccgt aacactgttg
     4021 aatttattga agtgctctgg cgcctcccat atcacagtct gtgcagaaag gcattgaggc
     4081 atcataccca ggcccgctaa cagccagaac agagcttgtt gcgatctgat gatacactta
     4141 gcgttcatct tgactgcaaa taggttttac tgaatttgtt gatattattt ccttgttttc
     4201 tcttttatca ttactggctt accggatctg tatttgattg tataatattc attctgatag
     4261 tacacatggt gcatgctgcc ctcaaactga aaaccctggc tggcttttat tgcagtaccg
     4321 tcaggatcag ggtagaaacc gaaaaagtct ttgagcatgg tctgcgcaaa cgctacacct
     4381 gatgatgatt ccctgttaca ccagtcgcgc tcggggatcc ttaccagcgc ctttttgttt
     4441 gttttgttct caccccacaa ttcatgagcc tgagcccata ttccttcgta tgtgacgggc
     4501 tcaacagcat ggtagaagtc aagcgcctgc tgcgggaaac ccatttgtgt aagagcatcc
     4561 attatatcaa ccggccaacc gtcaaaagca ccgaaaggac catgatcagc acggtcagat
     4621 ttgtcagcag caagatcctt gagcgactgt gccctcatcc agtgatcagt catcagttca
     4681 tcatagagga atcccagcat ttcactctta atattggcgg gtatatcgtc agggatgtac
     4741 ttgccgaaga atgaaaaatc aagacaatgg cgtacctcta tctttttgtt tccgggatag
     4801 aggctgttcc atacaccgtt tccagcataa agctgcagga tacgttttac catttcagaa
     4861 gccaggacat tcagggagtc agccttctgt tgttctccgc gatgctgatg gagcttggct
     4921 gtctcacgca tcatccatgc ataaccgaca ttgaacgatg gtacaatatg cttgtaggta
     4981 ggaacacact caagaaggtt ccattcatca tcgccgaaat cagccagcct gtaaacatca
     5041 tcagtgcagc cttcagtgcc ataaagagaa atgttcttcc agttcattga gtaatcatca
     5101 agatgctgca gtactgtttt atcgccaatt gtctcatcca gataagcata atctcctgaa
     5161 acagaaacat aatcgcggat aagttggaat agtgcccagt aatttgcgga atagccgttc
     5221 cccacacctt tgcccccgaa attatccttg ccgtagtatt tgctcgggtc tatcttaatc
     5281 catgaagaaa gatgctcttt catcattaca ggatcaacca cagcccagag tgttgaccac
     5341 agggtgatat cccagaagaa agtgatggat gcgccccaac ggggtccgcc tgtaagaaac
     5401 accttgctgt gctccggcag gttggtgttt atcaggtaaa gcattgtcag cggactggta
     5461 taatacactt tcctcgccac ggcgtcatca gtaacaagaa tggggaagca acccgagatg
     5521 agtgaattcc ccggcttgaa tatttcatcc catttctctt cccagctccc ttttatccca
     5581 ctgaaaagtg tatcaaaacc ggcagaccag tctgtaacat tctgttttat cgtttccgga
     5641 ttgtctccgt aagccatgag gtactttata gtcctcttct cacctggctt cagctcatat
     5701 ttccatgagg ctgttgctcc gcaattgctg cctgttagcc tgtcagggcc ggtaactaca
     5761 gaaaatgatg taattgcctg tgattcagta tcacggatat agacagtgtt gttttccaca
     5821 tttgtggtga attttttact gttcattata tctgcatcac caggccatct catctttact
     5881 ttctgcaaca ccggcttgcc atcaaccagt tcccatgccc agccctcttt ttcaaagaca
     5941 ttttcgccaa ggtgttccct gatgttctct acctcctcat tgcggttggt gactgtgcct
     6001 gacatgctcg gataagggta ccaccactgc cagtctcctc cgaacttgct gataaatcca
     6061 ataagatcaa gttcaagatt gacctccctg gtgacggtgt cgctgttggt tatttcaacc
     6121 tcccacatga ccgcatcctt ttcaaacagc attctcgttg aacttagaaa gtccaggttg
     6181 tcaatgctgc cttttctaag tgcctggtac ggctgccacc gccaggaatt cgccccgggc
     6241 gtctttccat taaccctcaa tgttcctgtg tgataccctc cggagaaagg tgcagagaca
     6301 aaccagctaa tggatgtcat atccctgttt agcagcgcct gacccctgaa gttcctgatg
     6361 gaaggttcca ttacaaccgt atccgttgaa acccatacgc cagacaactc ttcaagtgaa
     6421 gggatggcat ggctcaatcc ggtctggaca gatttactgt cattgcaacc ggcaaaccct
     6481 atcagtgccg gaagcagcag gataaatatt gactttttca ttttcggtct tttttatgtt
     6541 ctgacatatg ctttaatagt tgcacatagt ttgccttccg attcaccata tggcctgatg
     6601 gaataggctc cgacacttgc ggggacaatg aaagtctcgg catagtgaat aatgaacggc
     6661 tcaaatgaat tatcagggct ttcaataatg atctcgcggc cttcaacaag gttcagcacg
     6721 ttaactcctc cattggtatt atgatttact ttctttgaga accagtgcct tcgtgtttca
     6781 atgaactctc tttcatgcag accggttgat tcttctgtcc atccgtctcc ttcagagatt
     6841 ttccgaaacc ggttgatcag gttcttcctt acccagtctg ttgtcctgta ccattgtata
     6901 acattcattc catgatctat atttatcggt ctgggtctcc catcaatgtc aacacggccc
     6961 caatcccaga gcttgaatgt aaaaatatag gtggtggcgc tgatttccag cacgaggcag
     7021 ttctttcccg aagcatggac tgtacctccc ggaataagaa aatgatcatg cttcttcgcc
     7081 ggccacctgt tgatgtacct gacatcatca aacattgatc cgtcattctg cgccttattc
     7141 agatcatcaa tcatctcccg gggatttatg ccctccctca ggccaaggta caccgaagga
     7201 tcctcccctg tatctaatat ataataactt tcgtcctggg tatagtgcat accgaacttc
     7261 tcctggatgt attctgtgac cgggtgtacc tgcaggctta gattacctcc gtccatcgta
     7321 tccaggtaat caaacctgat tggaaactca tcgccaaatc tgccgtaaac ggcgtcaccc
     7381 agcagctctg ccggatgtgc aaatacaagg ttgactgaag gcatctcgaa gagtccctct
     7441 ccaaaatcaa gcatcaggct ctgttcctcg gggatgcagt taaagcacca tgagtagttt
     7501 tcaggttccg gatcaagggc aaacatcttc ttcatccatt gcccgcccca gatgccctga
     7561 tcaaaggtag ggacaatact gaacggtgtg gtcagggctt ttcgcagtga cctgctgaag
     7621 gcagaggctg ttatcattcg gggatcatcc atgtcagtgg tgtcaaggac gaaatcccac
     7681 ctggccataa gctttttctt gtgcctgtcg cacaccctcc agtcaacaaa aaaagctctc
     7741 ttgtactttg tctctgccag ctcctgcctg ttcctgagac cgagattccc aacaatgccg
     7801 gccctcatcc tcagctctgt ctcccggcgt ggcatatcag catagacaag gattaacggc
     7861 tcatcagcaa tgagtgatgc accgtagcca aagacaagca ccaatccttc agttaaatcc
     7921 cttacttttt cacgacatga gttcaacttt gcggtgtcag tgaggtctga gaaattgaac
     7981 cgggtaaggt atccgaaaat cctgtcatta gtgacatccg gccacgttat cctttgcacc
     8041 tcctcagggg agaaaagata gtcgttgctg gtaataacca gttcaggttc aagtctttca
     8101 gaaaatatac tgagcaactc gtcatagtaa attcccaggt tgcaatcaac cactatgaca
     8161 gttttctgtc cggagacact ttttattctt ccggataata caccgaagat actatcccaa
     8221 cccttgaaat agccttcgtt gaattccgca acacgtatta ccggtacttt attgaaatta
     8281 ggcttaagta tttcattttc gttaattgta tccttcattc tgagtgagtt tatgattgag
     8341 gtacctttct ttctgaggta tcgggaatag gagatggtaa gtctggatat tggctgttgg
     8401 ctttgctatt tggacgcgtt ccaaaagttt ccccatcctc acaagatcaa accacctgtg
     8461 gccctccata acaaactccc attgccgttc ctgcagcaag gcatccttga actcctggta
     8521 actcaatccg gcagcaaggt catcaaggtt tgccctttca cgtattgcat ttatagcgtc
     8581 ataagcttca gtggtaggtc ctttatgaat ttcattaagc gcttcggcat acatcagcag
     8641 cacatccgaa tagcggagat agatatagtc aacgtcagat tctccgttct gcctgcctcc
     8701 ttctgcctcc tgatcccagt atttccttat gaacggcccg tcaaaggtct caccctcaaa
     8761 ctcagtgata aatgttgctg ctttcctttt atcagtgtca gagaagctgt cataaaggat
     8821 gtccagcggc cgctcccatc cctggccgtt acgcgtgcca ttttcagaga tcgtggttgg
     8881 catgaggcgt actatgaact ggtttgtcgt ccatccttcg ccaagtgttc cgctgaaatt
     8941 gattgcccac aggatctcgc cggaatttgc attactcagc ttaaagatgt cagcatattc
     9001 cggctcaaga gtgtaatgac caagcacctt ctcaaggtat gtcctggcag aattgaaatc
     9061 cgcagcattg ttgttcagtg caccccttgt caggtacacc tttgccagta atgctgatgc
     9121 ggtcatgctt gttggcatgc ctgcaaccct gttggggaac gggtttacgg caagcagctg
     9181 ctctgctttc tcaagatcgc ttattatgct ttcatataca tcggctaccg gtgtcctggc
     9241 gatctgggtc tctgcagcag tctttgttga gaaaagctga agcggtacat caccgaacag
     9301 cctgacgagg tcaaagtaaa gcatgccacg caggaaataa gcttcaccct gaaggtggtt
     9361 cttgatctcc ggccttgaat aatctttaat attctctgat tcttcaagca ggacgttggt
     9421 cgaatttaat acctcatagc agccaaccca gatataatat accgttgcat tattgacatc
     9481 aaaagtgaac tgtgccagat caagcatgtc gattgaagga cccgtctcat aacagttatc
     9541 ggatgccaga tcctgcaaaa cccagtaatt attgaaaaac agccccccgc cggtccaggt
     9601 ccatgtgata agtccaccga acatgccatc ggcaaagctt accagatctt tctctgagga
     9661 aaagtaattg tcatagttca gttcagaacg caggtcctgt tccaggaaat cctggcagct
     9721 gccaaatgtt atagacagga aggtaaggaa tatgattatt tttttcattg ctttggattt
     9781 agcggttaga acgttgcgtt gatactgaac agaagtgatc tcgcggttgg gtatgcacca
     9841 tagtcagctc ccttcccaaa gacactccct ccgaaaatgg aaacttccgg atcatacccg
     9901 gaatatcttg taagagtata cagattgtat ccggtcagtg acagttggag gttacttatc
     9961 ttaatcttgc ttaggagctc ctttggcaga ttgtaggaga agattacctg tttaagcctg
    10021 gcatatgaac catcttcaac ccagacgtct gacattatta taccgtgagg cgaggcattt
    10081 gccctcggat attcattggt cgggttggtt tcggtccacc tctcaagcgc aacagcggag
    10141 ttattctggt acccgtcaaa gctctcaagc tggatcctgt tgaagttgac aatctcattt
    10201 ccgtaatcac cctggatgta aatatcaagt gaaaaattcc tgtatgagaa tgtattcctg
    10261 aggccaaatg agaaatcagg attaacattg cccagtctgt agtaatcatc aactgtcagt
    10321 tttccgtcag ggactttgtc caggtacttg cggtctcccc accttgctat cttggacggg
    10381 aagaagggta ctttagcagg gtcttcatca agctggatta tgccatctga cttgtacccg
    10441 tatatcacac ctatcggttg accctcactg atcatcgtcc agtcaaggat attcatgaca
    10501 gaagtcccat aaatgttgtc tgacttgtca aggttcgtca cttcattcct gttaaatgag
    10561 agattgaagc gcgtattcca cctgaagtcg ccttcaaagt ttaccgagtt cactgaaaac
    10621 tcgatacccc tgttctgcag gtcaccaacg ttagcccata catattcaaa tcctgacgat
    10681 ctgttcaccg ggagataaag cagaagatcg gatgtgttct tcaggtaagc ctctatggta
    10741 aactcgatcc ttgacctgaa gagactgagg tcaattccgg catcatactg ccgggtgacc
    10801 tcccatttca ggtcattgtt ctgaagagaa tagacgtatg tcccgataac ctcagcaccc
    10861 gggccttccc catagaaata ctgatgtgtt cccatgaggc ttaatgtggc acctgacggg
    10921 atgccttcgt tccctacact tccggcacta agcctgagct taagatcatt gattgctttg
    10981 gcattcgcca ggaagttttc tttcgagata cgccaggcag cagaaacaga tggaaagaac
    11041 ccccatttgt tgtcagttcc aaactttgac gatccgtcaa gtctccctga cactgtcatg
    11101 tagtatttgc tgtcaaaatt atacatgacc cttccaagcc ctgacaacat agcccatgag
    11161 ctgtagccgg acatgatcgt tttatcaagc cccataccaa ggttataata tgtaagcctt
    11221 tcatcttcaa agcgctcaac ggctatctca gctctgtctg acacaaattt ctgggcagtc
    11281 tgaccggcta gcacattgaa gtgatgcttg tcaattgcga acttataagt gagggtattc
    11341 tcaatcagcc acgtataacc ctggatgttg acattagatc ctttaccctt gtcctgccct
    11401 cttttaatat aacttgggat atactgctgc tccttgttga agaaggcgtc gacgccggcc
    11461 ttgaatttaa actgcaggtt ttttataata tcccagtcga tatagaaatc gcctatcatc
    11521 ctgtcagtgg tgataactat gtcagtttca ttcatctccg caaccgggtt tgggatagca
    11581 ttaatggtat tgctctcata ggtatagccc cccacctgag ttgagtcatt gacctcctgt
    11641 ccggggttaa acaacagagc cattgcggtc acactggagg ctacattcgg aacccctgtc
    11701 gtgactccgt tgctcacaga ttgcgacaga ttcaggttga cacccgtttt gaccttttct
    11761 gacaactcag tgtcaagatt taccctgaca gtacccttat taaagtcaga gttgtgaata
    11821 atacccttgc tggtaaccag gtttgcagat agaaaataaa ccgtactctc attgcctccc
    11881 tggagagata tctggctgtt tgatagcggt gcaatgcgga aaatctcgcc ctgccagtct
    11941 gttccttctc cgaaacggct gggaacagcg aagttgctgc cataggcaat ctgcggataa
    12001 tattttcttg cctcagtgac agcatcatta ccaagctcgg caagctctct cgcattgagg
    12061 agatcaagct tcttcgccag ttgctgcata ccgtacgaaa agttaagatt gacctgcagg
    12121 gcacctttct tccccctctt ggtatttatc agtataaccc cgttggcccc cctggctcca
    12181 tagattgctg tggcggaggc atcttttaat atctgcattg actcaatatc ctgaggattg
    12241 atgcttgata aaggattaat ggccgagcct tgtattgtac ccgttgtcag ttctgacgga
    12301 tcggtaatta tagggatgcc atcaatgacg taaagcggct cattcgtgcc gttgatggat
    12361 gtcgtacccc tgattcgtat tgataccggg gctccgggct ttcctgagga ctgactgacg
    12421 actacacccg atgcctgtcc ctgaagtgac tggtcaagcg atgtgatggc cggtgaatcg
    12481 atctccctgg ctttcaccgt tgagatggaa acagtcacgt cctccttcct ctgggtcccg
    12541 tacccgatca cgactatagc atccagctca gtcgtttccg aaaccattgt gaattcatag
    12601 tatgtgacac cttcaactat tcttgtactg ttttttgaga aacccacaaa tgaacaggtg
    12661 atagttctgg cagtaccctt gtcaatagcc agggtaaaca tgccgtcagc atccgtcatc
    12721 gtgccggtgg ttgttccctc aataacaacg gcgacggaag gcattggtgc tttgtcctca
    12781 tcaacaacct tacccctgat tgtcacctcc tgggtaaaag ctgtacttgc aaatagaata
    12841 aatgctaaaa acaggattga gtatttcatt ttcagaaatt tacactgatc aacataaagc
    12901 acataacgaa cttgcagaat ctcactgccc ttttcacaac agagaaatgc tcaaaatagc
    12961 cacgaagtct ctatttcatg gctgcctcca agacacccgg gctggaactg ccttccaggc
    13021 agccatgaaa agaatagtaa tttgtacaat tattgtattg tcagcagcga ttggcttatg
    13081 ttactgcaac taagtgaagc attggcattg acaattgctt ccatctcggc agtgccataa
    13141 ggcacgaatg ccccttttgg ctgcgatttg aatttattct caatgtcaag caaaccccaa
    13201 acctccaaat cccagtacat ggtcatttcc gtttcactgg cggcagtgat gttatagtca
    13261 taatactcat caatcgcggt atcgccctgt ttcagccttc ttaccagccg cacgattgcg
    13321 atggtatcgc cattggcagg ggtttctttg aaaacccagt agcaaaaatg atgaaagtca
    13381 gaggcataat cagcccccag gtcttcctga taggttccgt ccgcactgaa tctgtagaca
    13441 atggcagcat cagcggctgt aagaacgtcc acgcccatcc aggtacttgc ggtaaccttc
    13501 cagtcatatt ttacaaacgt tgacttgagc gagatgttta tgtttaccac cagacctact
    13561 tttttctgca ggtcttcgtc gtctaccatc tcaaactcaa atccaagtgt gtgcaggtcg
    13621 tcaaactcct gaaccgtata gctgaagttg taagtatacg tgttttcact cttaatgagg
    13681 tcagctttca catcagtgcc ttcatctctt acattatcaa ctaatttata aactttgaac
    13741 gacttgacac catcagcttt aattgttatc acagccgtag cattatcacc aatgttcagt
    13801 tggggtgtgg ctgtctgact gatggtcatt tcagtaacga tatccttctt gcacgaaaca
    13861 agaaaaacag taagtcctat caccataagg gcgattgcag aaagtaacag aattcttttt
    13921 ttcatgattt taagggttag gtttataatt caagaggtat ttattttcat atgttcaaat
    13981 gtatgaacat atatatattt atcaaaatta aaaaaaagca gattaaaaaa gaactgtttt
    14041 aataaaaaaa agtggagagg tcctgtaata actcaggaat actaaagctc tctctcactt
    14101 tcaacactgt aaacaaagct gtcagtacgg taaaaactca cattgtactc tatgggtctg
    14161 ctacccggat catagacaaa tctctttcgc ttcaggatcg gatccccggt atgaagattt
    14221 aacattcgtg ccagtgaccc atcagcagca actgcgctta tctcttctct cgacagtttt
    14281 gcaactgtgg catgttcctt ttccaggatt tcatatagtg gctggttgaa atcttctttt
    14341 cccgtaagac caattctcgg atgaaagaat gatactgaat aaacaaaagg accttcggca
    14401 ctgcctctca ggcgttcaag cctgaggata gtcttcttct ctggtatcct gaaaaaaccg
    14461 atatgttcct gctcaggctc tatccatgtc actttcagtt cataattgat gatatcaatg
    14521 cccagggtct tcatttcctg ggtaaagctt aaccagtttt tcaactttgt gtgtactgcg
    14581 gtattggcaa caatggtacc gacccctttc ttcctgatga gcaatccttc ataaaccagc
    14641 ttgttggttg cctgcctgag ggtgtttctt gaaataccaa gtagttttgc gaggtcaacc
    14701 tcccgcggaa ggaactttcc ttttgaatat tccgggtccc ttatcatttc acggagaaga
    14761 ttctcaacct gaatatgaag gggggaagag ctcttatgat caatcttgaa gttcatttcc
    14821 aaattcacga cctttctgct gtccggggca gagaagctat ttgaatgcaa gatcgtcatt
    14881 ttataaatac aaacaataat aagcggcatc cacttattga aaacaactgg catctatgac
    14941 aggaggattt taccacctgt tatttgatca tccaggcgcc agatgatttg attctggtat
    15001 gaagaaactt tacctttgat ataaaaggct gatcagcatg agctcacgtg acagaatcat
    15061 tgccgcaata aaccacaggc agcctgacag ggtgcctgtt gacctgggag cctcaacggt
    15121 taccggtatt tcggctatcg cctacaacaa cttgaaaaaa cagttgggca tcaaccagcc
    15181 tgcccgtgta tttgatgttg tccagcaact ggcaaatata gacatggagg tcatcaacat
    15241 gttcggtgtt gatgctcttg atatcaacag gatttctgca gaatccgggg agtggtatga
    15301 agttgaactt gctgacggca gccgggccga gtttcctcat tggtatcagc ccgtaaaaat
    15361 gcctgacggc tcatggcaga cattccatga tgacgggact gttttatcga ggatgccggt
    15421 tggtgcgaca ttcttcgatc agatgttttt tccatacgaa aacggatacc ctgactcttt
    15481 tgataacctg aatgatgctc tcacgaagat tagctgggtt gttcattcac attcatcaaa
    15541 cctcaacgac gaggagctga gggaaaagct tattaaactg aaagaattat ccggaaaagc
    15601 tctggtaatg tccggaggtg taaagcttct ggaacttggg ttcttcatca gaagaatgga
    15661 taatttcctg accgaccttc tgttggagcc ggaaaaactg tcacaaatgc ttgataagtt
    15721 tgttgaaatg cacctcgccg gactggaaaa caagtgcgac tcgctgggtg atatcgtcga
    15781 cgtgatacgc tttggcgatg acctgggaat gaaaaccggc cctttaatgg accttgatac
    15841 gtttcgcaga ttcttcaaac cgcgctacaa aatcctgtgt gattatgtga aaaaacatac
    15901 cggcatgaag atcttccttc actcatgcgg cagcataagg cagttcattc cagacctgat
    15961 agaggttggc attgacatac tgaatcctgt tcagaccaac tcacatgata tggatcctgc
    16021 cagcctgaag aaagagttcg gaaaggatat cgtcttctgg ggcggaggcg ttgatactgt
    16081 ctctgtgatt aaccatggca ctcctgaaga ggtaaggaag gatgttctga aacgttgtga
    16141 gatattttcg aaagacggag ggttcgtttt tgctcctatt cataatatac tttcagaagt
    16201 tcctcccatg aacattattg ctgcttataa tgccgtgagg gagttcaatg gtcagcccaa
    16261 ttgtataaat gactgaattg caggctataa ggaaattgat ctggttgcct gccatcaccc
    16321 tgttgatatt tgcctgctcc cacagtaagc aggtaccccg gaccaaaacg gtgaccctca
    16381 gcactgaagt tggcgagatt ctcatcatta ccataaaaca gacagcgccg ctatgtaata
    16441 catgcattta ggaatgtagg catattggtt agtggcaacc atacgccatc aaatgatagc
    16501 ccataatatc aactcattat aaacctaaag ctgcaatgcg ggttaagtat tgcaaccagt
    16561 ttctgatcag gccgggcaat gttatatcgt taccgtggat ggccagaacc tggtctgatt
    16621 ctatggttct gaaataggga aaaatacctg cccttttggt ttgcagagtc aggccgttgc
    16681 cggcaaagac attacagagt tttttaatgg tacctgacaa gtgaaaacaa tgtgaattca
    16741 gttatttgcc gaaaatagca gcataactac tccgataatg ggatgcccta atgagaatac
    16801 cattttatgc ttatttatca ctcctgaatt gtcgtcatat ttgtaatcat tgataataag
    16861 cgtttaaccc caccctcaac aggaggagag aaaactgttc aaggcatgac taaaaacaac
    16921 aacgcacccc tctctgacag gttcaacata ggacaggact gggcctcagt ggttacagga
    16981 ttcatactta tccttttcgt ggtgattaca ggttatgctg ttgccacccc gtcattcgga
    17041 ggaaaggcag gatggaactt tgatcatgac ttcacaggta tgttcggagc atcgtcactc
    17101 tggacctccc ttctttttac cctgatgatt tctgtggtta cggcaacaat agggatgctg
    17161 ttgagcggcg agaagatcag gaactatttt accggtttcg tcgccatttt cctgcttgtc
    17221 atccttgcac agtttgtctc gtcatttgcc ggtttcaaaa atctcggact ggagacagtg
    17281 ctcttctccc ttatcatagg ccttcttatc ggtaacttta tcaggctgcc ggaatggctg
    17341 cggtcaggtg tgcaaacgga gctttacgta aagataggac tggtactgct tggcgccacc
    17401 atcctgttca gggatatcct cacggccgga gcattcggac tgatccaggc tgtgattgtg
    17461 gtgacggttg tgtggtattt tgccttctgg ctatcgcgaa aaatgaaggt tgacgatgag
    17521 ttctccacca tgcttgccag tgctgtctca atctgcgggg tgtcggcagc cattgccact
    17581 gcaggggcca tcaacggcga caagaagaaa ctctcattca taatctccct ggtactcatc
    17641 atagccatcc cgatgatgat tttcctgcct gtcattgcca aatggatggg actctccgag
    17701 gttgtcacag gcgcctggct gggaggtacg atagacacca cgggtgctgt tgttgccgca
    17761 ggaaccatag cgggtgagac agggctcaaa tatgcaacaa tagtcaagtt ctcgcagaac
    17821 gtactattgg ggctggctgc gtttgcaatc tcaatcttct gggcatacag aagcaggggt
    17881 gccggtgaaa aaggccagca tgtgccggtg aaaatcatct gggatcgttt cccgaagttc
    17941 gttcttggtt ttatcctggc ttcactgatc ttctcattca tcctcagtcc cgggatggca
    18001 gagtcgtcag gaaaggtcat caaatcattc agcaccttct ggtttaacct ggcatttata
    18061 agtatcggac tggaaaccag attcgccgat ttcaaacaaa tggaaagcag gaagcccttc
    18121 tatagcttcc tgatagcaca aaccttcaac ataatattta ccctgggagt ctcctgcctg
    18181 ctgttccagc taataaatac caacataaaa tgaataaagt aagaaacatt atctttttgc
    18241 tgattaccag cgccattatg gcattaagct cctgcgatgg aaagaaagag gtgattatca
    18301 ataccgggtt tggtgagata cacgttgctc tggatctgca gaatgcccct gtcacttccg
    18361 ccaattttct aaggtatgtt gatgccggcc tgtttgacag tgcatgcttt tacagggttg
    18421 tcagacctga caaccagcag aatgattcga tacgcattgc agttatccag ggcgggcggt
    18481 acaatcagga agaaaccgac gggtttcctc cgatagccca tgagacaacg gagatgaccg
    18541 gaatcaggca cctcaacggt acaatctcaa tggcaaggtg gactcccgga acagccacat
    18601 cggagttctt tatctgcgtg ggtgatcagc ctgaacttga ctacggagcc aaaaggaatc
    18661 ctgacggtca gggctttgcc gccttcggtc aggtaacgaa gggaatggat gtggtcatga
    18721 agattcacag cattgaggcc cctgcacagt acctcgacaa accgattctg atatacagca
    18781 tcaagcgcaa atagtaattg ttgcagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnctc gacaaaccga ttctgatata cagcatcaag cgcaaatagt aattgttgca
    18961 gcgcaacgtg ctccgggctt gcaggattgg gattcactga tatctttgtc tgtgaaaaac
    19021 tgctgacaga gcgttagaca aaaacatatc caatgaaata cgtaggagca cacgtaagtg
    19081 cctcaggcgg ggtagaaaat gcacccctta atgcttataa tataggagca aaggctttcg
    19141 ctttctttac caaaaaccag cgtcaatggg tggcgccgcc tcttacatcg aagagtattg
    19201 aaggctttcg caaaagatgc gaagagtatg acttcggacc tcaccagatc ctgccacatg
    19261 acagttatct gataaacctt gggaaccctg acccggaagg actgggacgc tcacgccact
    19321 ccttcattga tgagatgaag agatgtgaac tgctgggact tgacagactg aacttccacc
    19381 cgggaagcca cctgggcaag atgacagaag aggagtgcct tgacctgata gctgaatcgg
    19441 tgaatattgc ccttgacaaa accgctggtg tcatagcggt aatagagaat actgcaggac
    19501 agggaacaaa cctgggctac cgttttgagc acctccgtca tataattgac agagttgatg
    19561 acaaaagcag agtaggagtg tgtatagaca cctgccatgc attcacctcc ggatatgacc
    19621 tctcaaccga agagggtttt gaaaaggtct ttaacgaatt tgaaagggtg gtcggattca
    19681 actatctgaa aggaatgcac ctgaatgatt caaagaaacc gttcggatct cgtgttgaca
    19741 ggcatgacaa cctggggaaa ggcctactgg gtaatgaggt gttcagactg ataatgaatg
    19801 atccccgctt tgacggcatg ccacttatcc ttgagactcc cgatgagaat ctgtgggtag
    19861 aggagataaa atacctttac tctcttcaga acaggtaaac ctcttcgctc cgcgttatta
    19921 ttgaccggtt atcagccctg gggctgatgc ctggcagata atccggtcat ctgtatgata
    19981 tttcgttttt acgctttttt ttacctttgt cgccggcaat gatcctttca ggattatcag
    20041 agccgggaag cgtgccattt atgacaaatt acattattct tgccctttgt gtgattatca
    20101 ttctctccta tctgtttgac atttcaagca gatactcgag ggttccgggg attatcctgc
    20161 ttataggcct tggtattgcc ctgcaactga tatcactgtc aacaggcttt gatattccca
    20221 acctgaaacc catattgcca gtgatcggga cattgggact ggtgatgata gtgcttgatg
    20281 ccagccttga cctgaagctc gaaaagaaaa agtcaagggt tcttattaac actgtattgt
    20341 cagcccttgt attgcttatt ctggtggtta caataggatc actggtgctt gtaaaaatat
    20401 ttggtcatac cccgatttct gcagtgctga acatgatacc cctcggaata atcagcagcg
    20461 cggcggcaat tccctctgct gaaggattga gttccaaaga gaaggagttt attgtttatg
    20521 aaagctcctt ctcagatatt ctgggtatca tggcctttga ctttatactt ttaggtcagt
    20581 ctgagatgaa cctgggggtc ggtaattttc tgatcgcagg ctttctgaca atagtgattg
    20641 ctgctgtcac tacagtagga cttgcatggt tgctgcataa ggcaaattat catgtcaatt
    20701 atgtaataat aatgacattc gtcgtgctcg tatatgtact ggctgaactg taccatctcc
    20761 ccgcgcttct gctggttctg gttttcgggt tagctctctc caacaacaga atagttgaaa
    20821 acacatttat cagcagatat attgaatttg gcaagtttaa ccgtgatctg aatgctttca
    20881 gaagaattct cggggagttg actttcctgg tgagatcatt cttttttata atatttggtt
    20941 attatacctc cgtggagggt gtcttctacc cggagaacct gattacagca ttcatcatta
    21001 ctgccggaat actgcttatc agatggctat ttttctattg catattgcgt attaaaagcc
    21061 tgcccctggt tctttttgcc ccgcgtggac tgatcaccat ccttctcttc ctcagtattc
    21121 ctgctgcata catgatcccc gagataaaca ccgaagtcat tacactggtg attcttatga
    21181 cactcctcgc aatgatgctg ggcaatataa tctctccacg aaaggctaaa agggtagtat
    21241 cagccgctaa aacagatcct caggtaaaga atgttttagg tgtttttgat tatgc
//
LOCUS       DAOPBK010000018        21201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2619_length_21201_cov_6.452048, whole genome shotgun sequence.
ACCESSION   DAOPBK010000018 DAOPBK010000000
VERSION     DAOPBK010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2619_length_21201_cov_6.452048"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="PLA17_01995"
     CDS             complement(<1..1879)
                     /locus_tag="PLA17_01995"
                     /inference="COORDINATES: protein motif:HMM:NF010380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HPK84214.1"
                     /translation="MTDSRFQPDEWKIIETRFDAVRGRDSESIFALGNGMMGQRANFE
                     ETYTGDSLRGNYVAGVYYPDKTVVGWWKVGYPEYFAKVPNAPSWTNIIVRIDGEELDL
                     AKCKVKSFRRELDMKEGVLTRIFTAVMPSGREAEVTARRFLSMDEPELAAIRYSVRIK
                     GNGAKVELIPWLNADVYNEDANYDEKFWENESSSHDGRRSVVVAQTRKTAFVVATAME
                     NSFTLNGKPLKGEPVITTTDKTAHCAFISACSDGDIFTVTTYAAVLSSFNHPVEGIAE
                     KAMVSAGKAAAKGFEALLDAHRNAWAQKWEHGDIVIRGDIAAQQGIRFNIFQLNQTYT
                     GEDPRLNIGPKGFTGEKYGGSTYWDTEAYVLPFYLSTADTHVARNLLLYRYKHLGKAI
                     ENAKKLGFTGGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAYAIFNYVRHTGDQDY
                     LSEYGLEVLIAISRFWAQRTNWSQEKNRYVILGVTGPNEYENNVSNNWYTNILACWTL
                     KYTSDMLDWLRSSSRVKYNAIVARTHLDTEAEPYHWKEIIENMYLPVHHDLGIFLQQD
                     GFMDKVQQTVKDLDPAERPLNQHWSWDRILRSVFIKQADVLQGIYFFEDDYDEDTIRR
                     NF"
     gene            complement(1886..2536)
                     /gene="pgmB"
                     /locus_tag="PLA17_02000"
     CDS             complement(1886..2536)
                     /gene="pgmB"
                     /locus_tag="PLA17_02000"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HPK84215.1"
                     /translation="MMDLKGCIFDLDGVIVDTAKYHYMAWRRLAKELGFVFTLEDNEA
                     LKGVSRMASLEILLSKGNIHATRQEKEELAARKNGWYVEFISGMKPDEILPGSVKLLR
                     SLRKEGILTAIGSASKNAGTIIDKINLRDMFDVIIDGNKISKAKPDPEIFLRAVQEMK
                     LSPPHCIVFEDAQAGIEAAIAGGMKCVGVGSPELLGRADLVIPGLNKITLEQLRAL"
     gene            complement(2536..4071)
                     /locus_tag="PLA17_02005"
     CDS             complement(2536..4071)
                     /locus_tag="PLA17_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK84216.1"
                     /translation="MIYRRKPRLSGWQIWNMSFGFLGIQFGFALQNGNVSRIFQTLGA
                     EIDNIPILWIAAPVTGLLVQPIVGHMSDRTWNRLGRRRPYFLTGAIFASLALAIITNS
                     PTWIFATVMLWILDASINISMEPFRAFVGDMLSSEQRTTGFAMQSFFIGVGAVIGSFL
                     PWILANWFNVPEVPAEGKLIPFNLKLSFYIGSAVLILAVLWTIIRTKEYSPAELEEFE
                     KGERIARGEPPEEKTVSEQAETVRNINFYRPGIIWLLAGLALTFILTQVNLEKELYVV
                     TGGISLFGLLQIIAGFLKNGGKGNNGLNSILTDLKNMPTTMGQLAVVQFFTWFALFSL
                     WIYTTSAVTSKIYGTSDTTSLAYNEGANWVGVLFGIYNGFAALVAFLLPVLAKHTSRK
                     ITHAICLLVGGVSLMSISLFNSPEMLILPMLGIGLAWASILSMPYAILTGSLPHNKMG
                     VYMGIFNFFIVIPQIIAASILGSMVKHLFSGNTMLALVTGGISMIIAALAVNFVKDVD
                     DRR"
     gene            4354..5964
                     /locus_tag="PLA17_02010"
     CDS             4354..5964
                     /locus_tag="PLA17_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HPK84217.1"
                     /translation="MGDFNYEKLFQHGRDVTKYRRISADHVRMTECCGRKILHVQAEA
                     LRILARQAFIDINFYLHTSHLEKLSMILRDPEATDNDRFVAWMMLRNSVVSAEGKLPW
                     CQDTGTAIVMAWKGEDVYTGIDDALHLSRGIWETYNEKNLRYSQIVPSSMFAEKNSGS
                     NLPAQIDIASCQGGDYRFLFIAKGGGSANKTLLYQESNSLLNEASLSRFIREKIEDLG
                     TAACPPYYLALVIGGTSAEMTLKTVKLASTGYLDNLPISGNSSGRAFRDPEWEEKIVK
                     ICQEYGVGAQFGGKYFVRDVRVIRLPRHAASCPVGIGVSCSADRNIKGKITTEGIFIE
                     QLEREPGKFLPEKAPELDKPVEINLERPMREILAELSKYPVKTRLSLTGTLIVARDIA
                     HARIKRMLDEGKPMPGYFKNHPIYYAGPAKTPEGMASGSFGPTTADRMDPYVDLFQSL
                     GGSMIMLAKGNRSKQVTDSCKKHGGFYLGSVGGPAAILAAENIVSVKVVDFAELGMEA
                     VRKIVVKNMPAFILTDDKGNDFYSGNSR"
     gene            complement(6076..6780)
                     /locus_tag="PLA17_02015"
     CDS             complement(6076..6780)
                     /locus_tag="PLA17_02015"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPK84218.1"
                     /translation="MEEKVYSMPRIGDKAPEFRAVTTQGEINFPNDYKGSWVILFSHP
                     ADFTPVCTSEFMTFATMAEKFEKANTKLVGLSVDGLYSHIAWLRTIKEKIEYKGMKDV
                     EVKFPLIEDITMEVARKYGMMMPGESDTKAVRAVFFIDPKGIIRTILYYPLSLGRNFE
                     EIYRVVIGLQTADKFSVALPADWQPGDDVIVPTAGSCGVAKERMENKDEMKCYDWFFC
                     TKKLDKQKILDTILKK"
     gene            complement(6884..7105)
                     /locus_tag="PLA17_02020"
     CDS             complement(6884..7105)
                     /locus_tag="PLA17_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84219.1"
                     /translation="MIRLLSYQPWHLSGPGIFPGTTKTLRETMTRFHEKEKEETCNII
                     LQTPDDQNEFLKLHARNLQHHPSDPDGEV"
     gene            complement(7102..7539)
                     /locus_tag="PLA17_02025"
     CDS             complement(7102..7539)
                     /locus_tag="PLA17_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPK84220.1"
                     /translation="MENYRTRLRESGLRVTPQRLTVYEAVDVLRNHPTAEEVSHYISK
                     KHPEIALGTIYKTLETLAEKNLLKRVKTDSGLLRYDAADESHHHIYCTNSDRIDDYYD
                     TELTKMLHDYFETNRIPDFRIEDIKLQIVGNYTDNRKNTRKKR"
     gene            7883..>8366
                     /locus_tag="PLA17_02030"
     CDS             7883..>8366
                     /locus_tag="PLA17_02030"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HPK84221.1"
                     /translation="MTNRKSLALAALLLIQTAFVNAQVLEPVAWSFKSEKIAEKSYDI
                     VMTATIDDTWHLYAMDLPEGGPVATSFTFETPEGYTLEGKPVAVNKPEVKFDNSFGMD
                     IGMHSGTVEFRQKISVNKLPVTVSGFVTFMSCDDTQCLAPRDLEFSVNIGDEVAASAD
                     K"
     assembly_gap    8367..8466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8467..10098
                     /locus_tag="PLA17_02035"
     CDS             <8467..10098
                     /locus_tag="PLA17_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPK84222.1"
                     /translation="DLEFSVNIGDEVAASADKVDEAAGTTLPVDKPRKGFLKFFLLSM
                     LAGFAGILTPCVFPMIPMTVAFFSRGSGKKSSAVGQALIFGLSIILLYSSLGIIVSLT
                     SAGAGFANTLSTHWIPNLLFFSLFLIFAASFLGAFEIVLPSKWVSSSDLKVDKGGALA
                     AFFLGLTTVLVSFSCTGPIVGALLVEAATGDVLRPTIGMFGFGLAFAIPFTLFALFPS
                     WMQKLPKSGGWLNSVKVVLGFIMLAFSLKFASTIDTVYNLGILSRDIYLAIWIVLFVL
                     LGIYLMGKIRFVHDSDVPHIGVFRLLLIIASFTFALCLVPGLFGAPLTRISALLPPRE
                     TSGFNLLKAISENRATAPATEFPGMVPGIAVAGASGFDTPASGAVSAAGTALCEEPKY
                     GDFLHFEYGLQGYFDLDQGLACAKKLGKPVLIDFKGHACANCKEMDARVWSDPAVQQR
                     LRNNFILVALYVDDRTKLPENEVFVSKVDGKEKKTMGKKNEDIEISMFNTNSLPLYAI
                     VDTDGKPLIESRGTDFDVQAYIEWLDRGAEAFRNQ"
     gene            complement(10101..>10661)
                     /locus_tag="PLA17_02040"
     CDS             complement(10101..>10661)
                     /locus_tag="PLA17_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HPK84223.1"
                     /translation="HDPAELIRLHGGKKRFVEHLDSLFTMYLPDSFFAETEDITREGI
                     IGNYVHGNEPSHHVPYLYNHAGAPRKTQYRVRHIMDTKYLPAPDGLSGNDDCGQMSAW
                     YIFSALGFYPVAPGSDRYDLGSPLVKEATISLENGKKFTVRAVNQGPNNIYVKKAELN
                     GKKLDRHYITHDEIMNGGSLVFYMSK"
     assembly_gap    10662..10761
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10762..12309)
                     /locus_tag="PLA17_02045"
     CDS             complement(<10762..12309)
                     /locus_tag="PLA17_02045"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPK84224.1"
                     /translation="MSIKIHAALLLSMVTILCAGQDADILKYVNPFIGTDKMGHTYPG
                     ATVPFGMVQLSPDTDTLPYEINGSYNRDVYRYCAGYQYTDKTIVGFSHTHFSGTGHSD
                     LGDILLMPTTGPLQLNPGTADAPEKGYRSRFSHSNEKASPGYYSVLLEDHDILAELTA
                     TTRTGVHRYTFNKGGDAHLILDMVHGIYNDAGKNVWTFIRVENDTLITGFRQTSGWAR
                     TRSLYFAIAFSKSFTSYGFRDDSPARVYKGFWRRFDQRTNFPEAAGRNIRGHFDFNTE
                     PGEQITVRVAISPVSSAGAMANMIAEAPRADFDAARLAAEVLWRKELEKVKVEMMTEG
                     QMTSFYTSLYHTFLSPTIYTDTDGSYRGLDQNIHHAEGFTNYTTFSLWDTYRALHPLF
                     TIIQQERTADMINSMLAHYDQSVHHMLPVWSHHANENWCMIGYHSVPVIADAIVKKIG
                     GFDHARAAAACVTTARNGWYDGLDDYMKLGYVPDERTGSSVSVTLEYAYDDWCIAQAV
                     SKLNPGEL"
     gene            complement(12603..14135)
                     /locus_tag="PLA17_02050"
     CDS             complement(12603..14135)
                     /locus_tag="PLA17_02050"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPK84225.1"
                     /translation="MDTLIITGSGLTVNDVVNVARHGQKVELHPDARKRILECRAMLE
                     KKIAANEIMYGVNTGIGEFSEIVLNDEQIKEFQKYLVYNHAAGIGDPAPIEIVRGAML
                     SRINVHAHGNSGCRLEITETYVEMLNKGVTPYACQKGSVGACGDLAPMSQIALLLMGE
                     GKAFYKGELLSGAEAMKRAGIKVPGLLVRDGLAVINGSNMLTAMSAIWLVDANNWLKQ
                     AEIAAAMSLEALKANMKPYTPLLHEVRGFKGAMRSANAINKCVTGGDLKEGRIKSKVQ
                     DAYSMRSTPQVIGAAHDMLGYARSQVEIELNGVCDNPVFFPDKDLQLSGANFQGTPVA
                     VPMDMAGATITMVCVLSERRLNRLNNPALNVGLPAFLTKGAGMFSGLMLSQYTADMLI
                     VEQRILSAPASIQSIPAAADQEDFVSMGMNTAIKNNQILDNAYGILGIEYMAAAQALD
                     FRKSEYSFGTGVQKAKDVIRKHVQFLDIDRPLYEDHTTMKELVKSCEILHEVEKTVGS
                     LE"
     gene            14446..15819
                     /locus_tag="PLA17_02055"
     CDS             14446..15819
                     /locus_tag="PLA17_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HPK84226.1"
                     /translation="MKSRFTIILLLVIVPAAILLSLAAFNIIRSLPQEDLKGRYQYKD
                     FESALKCRSCHPGIYEQWSQAMMSQAYTHHWDEIEYFELAVPHAEVNPSLKDAVDGCN
                     GCHTPMGYMAGVFPPPRPSEKSMANESVSCEVCHLTRRAQVDPPFNFSYYIEPGRVKY
                     SGREPEIQSPAHKIVQNDFFRTTEFCGNCHNEKNPYGIWVKSTQLEWMEGPYAREGVR
                     CQDCHMPEGQYTLALMGKERDDARLHLFHGAHDPGKVRGTIELRIEPDVRLAEPGENV
                     VFTVALFNQKTGHKFPTGSVEDRIAWLDVEATDAKGKTYHLEVDRKGFEGEEYTISGD
                     YLAYQDMAMPLKLNDFKGVQRDGIPYGNRIFRMPFFDEQGNMTILQWNTFSLGVDYRI
                     GPRETKVEKFTFKIPWDAAPGEMKVRAVLNYQLLVKPVADFLKVPPDESEIIMVNEHS
                     TTVGILP"
     gene            15844..17043
                     /locus_tag="PLA17_02060"
     CDS             15844..17043
                     /locus_tag="PLA17_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84227.1"
                     /translation="MKTSFIARAAILTVTIIMLLPYKQMSAIPAFARKYQISCQVCHT
                     PVMPRLKAFGDDFAGDGFRMTEYESPRHFIQTGDDRLSLFRELPLAFRIDGHVTANFN
                     GEGSVDFAAPFMLKILSGGELSDRLSYYFYFMLDERGRVAGLEDAFIMYKDLFRTGIN
                     IYLGQFAVSDPMFKGEMRYTIENYHIYATAPGQSSVNLKYDKGILLDKGFKTGTTVVF
                     EVISGTGLGSADHWYVFDKDKYKNMLLKVTQDIGEYASLGFFGYTGKELLWTGTGDIV
                     SSVKMYGPDLVLDFNEKLIIGLQYLWRTDSDVYLNPGQPVRKNVLTHGGFAEVIYSPK
                     GDMSTWYLNGLLNWVESDLDMLDYRSATLHAGYIIRRNVRLVTEYTRVFSGNPYGKLS
                     GGFVSAF"
     gene            17112..18998
                     /locus_tag="PLA17_02065"
     CDS             17112..18998
                     /locus_tag="PLA17_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPK84228.1"
                     /translation="MKKFMVFAAILFLASCTGKKEVKQYTIDQFYKNVSIQFSDFSPD
                     ETKGLVTSNETGIYNLFEIDFADGTRKQVTFSDTESLFGIGYVHGTGQILYSSDKGGN
                     ENDHIYLLNEDGTSTDLTPGEAVKASFSGFSLDKKVIYLLSNKRDARFFDLYRMNVGE
                     WKEEMVWENSMGLEYAGASRDRSIIAMVKPITTSESQLFIHDLATGETKEVSEPGKPG
                     MYMASGFTNDNAWFFYTTDAGREFSYLMRYSLATGERELLYETSWDVTGSWLSRNEKY
                     QLIGINEDGRIQVNITDYATGEKVTHPEVPGASVNELAISESEKYLLLSLGTSKTPGD
                     LYLYEFGSTGIKKLTDVLNPEINPADLVDAQVVRFSSFDGLEIPAIYYKPLTATKKNK
                     VPALVWVHGGPGGQTMAYYNPIIQYLVNHGYAVLGVNNRGSSGYGKSFYRMDDRNHGD
                     KDLKDCIWGKKWLQAQEYIDGEKIGIIGGSYGGFMTMAAMTSAPEEFRVGVNLFGVTN
                     WLRTLKSIPPYWASFRDALYTEMGDPFTADSVRLYNISPLFHGQNVKNPVMVLQGAND
                     PRVLQVESDEMVEAIRAAGVPVEYVIFPDEGHGFIKKENEIKAYSQILQFLDKYLKGA
                     GEEG"
     gene            complement(19158..20390)
                     /gene="hydF"
                     /locus_tag="PLA17_02070"
     CDS             complement(19158..20390)
                     /gene="hydF"
                     /locus_tag="PLA17_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPK84229.1"
                     /translation="MKAKGRDTKPHVGIFGRRNVGKSSLINALVGQDIAIVSEIAGTT
                     TDPVKKSIEIHGLGPLILIDTAGIDDSGELGELRIKKTMDALKLIDLAILVLSDNSFD
                     DHEAELTQEFGRLDIPYVIVHNKSDLSPVKDEFRKKIRNLTGREVAAYSALFPDRYNP
                     ALLEMIKEAMPPIAMKSHGLIGDLVNPGDIVLLVTPVDIEAPEGRMILPQVQVIRDTL
                     DNDCTAIILKETDIESFLEKTGIRPQLVVTDSQVFGKVNAVIPRDIPLTGFSIVLARQ
                     KGDFEEYLRGTPKIGELQDGDRVLLLESCTHNVSCEDIGRVKIPRWLTNFTGKKLDFD
                     VVAGLDTFPRPIEDYALVIQCGGCMVTHRQLVNRLREPKKHNVAITNYGMAIAYLHGI
                     FDRAIEPFTGKRQEAGDL"
     gene            complement(20452..>21201)
                     /locus_tag="PLA17_02075"
     CDS             complement(20452..>21201)
                     /locus_tag="PLA17_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84230.1"
                     /translation="RETSERLEAMKKQWSEHEADVKNSIIRICDVNHVNYVEKVPFRG
                     NPDNTIEISGEYIIFDAKSPAGDDLTNFPNYIKTQAENLKKYASQDNVRKSIFLVVPT
                     NTIDSIRQLTYRLGDYDVFVITKDALEPVILSLKKIEEYDLAEKLSPEDRDSICRIIG
                     KFAHTAKRRIQVDQFFSEEFIDIVLRSQKELPEDLRKQVIEFEKSEKLNPPTERRAKI
                     ISIDELQKKSEEIDAEAQSRKLSPHVSNDES"
BASE COUNT         4915 a         5231 c         5323 g         5532 t
ORIGIN      
        1 cgaaattgcg cctgatggta tcctcgtcgt aatcatcctc gaagaaatag ataccctgga
       61 gcacatcggc ctgtttgata aagaccgacc ggagaatgcg gtcccatgac cagtgctgat
      121 tcagaggcct ctccgccggg tcaaggtctt taaccgtctg ctgcaccttg tccatgaatc
      181 cgtcctgctg caaaaaaatc ccaagatcat gatgcacagg caggtacata ttttcaatta
      241 tctccttcca gtgataaggt tctgcctctg tatcgagatg ggtccgggca acaatggcgt
      301 tatatttcac cctgcttgat gacctgagcc agtcgagcat gtcagaggtg tatttcaatg
      361 tccagcatgc caggatattt gtgtaccagt tgttgctgac attgttctcg tactcattcg
      421 ggccggtaac accaagtatg acatacctgt ttttttcctg tgaccagttg gttctctgcg
      481 cccagaagcg cgaaatggct ataagcacct caaggccata ttccgaaaga taatcctggt
      541 ctcccgtgtg cctcacatag ttgaagatag cataggcgat ggcgccgtta cggtgtatct
      601 cctcaaaggt tatctcccat tcattgtggc actcctcgcc gttcatcgtg accatcgggt
      661 atagtgctgc cccgcccgtg aatcccagtt tcttcgcatt ctcaatggcc ttgcccaggt
      721 gtttatacct gtaaagcagc aggttgcgtg ctacatgtgt gtctgccgtg ctcaggtaga
      781 agggcaggac gtaagcctcg gtgtcccagt aggtacttcc tccgtacttc tctccggtga
      841 atcccttggg cccgatgttc agcctggggt cctcaccggt atatgtctgg ttcagctgga
      901 atatattgaa ccgtattccc tgctgtgctg ctatatcacc acggatgacg atatcaccat
      961 gttcccactt ttgggcccat gcattacgat gtgcatcaag caatgcttca aatccctttg
     1021 cggcggcctt gccggccgaa accattgcct tctcagcgat cccctccacg ggatgattga
     1081 aagaggagag taccgcggca tatgtggtca ctgtaaagat gtctccgtca gaacaggcag
     1141 atatgaatgc gcagtgagcg gttttgtcag ttgtggtaat gacaggttcg ccctttagcg
     1201 gctttccgtt cagggtgaag ctgttttcca tcgcggtggc aaccacgaaa gctgtttttc
     1261 gcgtctgcgc aacaactact gagcgtctgc catcatggga agatgattcg ttctcccaga
     1321 acttctcatc ataattggca tcctcattat atacgtcggc gttcagccag ggaataagct
     1381 caacctttgc gccattccct ttgatccgca cactgtacct gattgcggcc agttcgggct
     1441 catccatcga gaggaaccgc ctggcggtta cctcagcctc cctgcccgag ggcataactg
     1501 cggtaaagat tcgcgtcagc accccttcct tcatgtcaag ctccctgcgg aagctcttca
     1561 ctttgcattt tgcaaggtca agctcctcac cgtctatgcg tactataatg tttgtccatg
     1621 aaggcgcgtt gggcaccttg gcaaagtatt ccgggtaacc gactttccac catccgacaa
     1681 cggtcttgtc agggtaatat acaccggcta cgtagttgcc ccggagtgaa tcaccggtat
     1741 aagtctcttc aaagttagcc ctctgtccca tcattccgtt acccagagca aaaatgctct
     1801 cggaatcgcg gcctctcacg gcgtcaaacc gtgtctctat gattttccac tcatcgggct
     1861 gaaaacgtga atcagtcatt gtgaattata aagctctcaa ttgttccaaa gtaattttat
     1921 tcagaccggg aatgacgaga tcagccctgc caagcagttc ggggctgcct actcccacac
     1981 acttcattcc gccggcgatt gccgcctcta ttcctgcctg cgcatcttcg aaaacaatgc
     2041 agtgcggtgg cgacagcttc atttcctgta cagccctgag gaatatctcc gggtcaggtt
     2101 tggcctttga tatcttgttg ccgtcaatga tcacatcaaa catgtcacgg aggtttattt
     2161 tgtcaatgat tgtcccggcg tttttgcttg cagaaccaat agcggtcagg atgccctcct
     2221 tcctgagact cctgaggagc ttcacgctgc ccggaaggat ctcatcaggt ttcattccgg
     2281 agatgaactc aacataccag ccgttcttcc ttgctgccag ctcctccttt tcctgtctgg
     2341 tggcatgaat gtttcccttg ctgagaagta tctccagtga agccatccgg cttactccct
     2401 tgagagcctc attatcttca agcgtgaata caaaaccgag ctcctttgcc aggcgcctcc
     2461 aggccatata gtgatacttg gcggtgtcaa cgataactcc gtcgagatca aaaatgcacc
     2521 ctttcagatc catcatcacc tcctgtcatc aacgtctttg acgaaattga ccgccagagc
     2581 tgcaattatc attgagatcc ctccggtcac cagcgccagc atcgtattgc ctgagaaaag
     2641 gtgcttgacc atgcttccga ggatgctggc tgcaattatc tgcgggataa cgatgaagaa
     2701 gttgaagatg cccatataga cacccatctt gttgtgaggc agtgaccccg tcagtatggc
     2761 atagggcatt gagaggatgc ttgcccatgc cagtccgatg ccaagcatcg ggagaatcag
     2821 catttcgggt gagttgaaca gactgattga catcaggctc actccaccca ccaggaggca
     2881 gatggcatga gttatcttgc gtgatgtatg ttttgccagt accggtagca ggaaggcaac
     2941 cagtgccgca aatccgttgt aaatcccgaa aagcactccc acccagttgg cgccttcgtt
     3001 ataggcaagg gaggtggtgt cactggtgcc gtagattttt gaggtgacag ccgatgttgt
     3061 atatatccac agggagaaga gggcaaacca tgtaaagaac tgcaccacgg caagctgtcc
     3121 catggtagtg ggcatattct tgaggtcggt aagaatgctg ttcagcccgt tgttgccttt
     3181 gccgccattc ttcaggaatc ctgcaattat ctggagcaat ccgaagagag agataccacc
     3241 ggtaacgacg taaagctcct tctcgagatt aacctgtgtg aggatgaagg taagcgccag
     3301 gcctgccagc agccagatga tgccgggacg gtaaaaattg atattcctga ccgtttctgc
     3361 ctgctctgac acggttttct cttcaggagg ttcgcctctg gctattcttt ctcctttttc
     3421 aaattcctca agctcggcag gcgagtactc ctttgtccgg ataatggtcc agagtactgc
     3481 caggatcaac accgcagagc cgatatagaa tgagagtttc aggttgaagg gtatcagctt
     3541 gccctcggcg ggaacctcag gtacgttgaa ccagttggca agtatccagg gaaggaagct
     3601 gcctataacg gctccaacgc cgatgaagaa gctctgcatt gcaaatcctg ttgttcgctg
     3661 ttccgatgaa agcatgtcac ccacaaatgc ccggaacggc tccatggaaa tattaatgga
     3721 agcatccagg atccacagca tcacagtggc gaaaatccag gtgggtgagt tggtgatgat
     3781 ggcaagtgcg agagaggcaa aaatggcacc ggtaaggaag tagggacgac ggcgcccgag
     3841 acggttccag gtacggtcac tcatgtgccc cacgatgggc tgtacaagca gtccggttac
     3901 gggagctgct atccagagta tcgggatatt atcaatctcc gcacccagag tctgaaaaat
     3961 acgactcacg ttgccgttct gaagggcgaa accaaactgg atgccgagga atccgaagct
     4021 catgttccaa atctgccagc ctgacaaccg cggcttgcgt ctgtagatca cattcataat
     4081 agttttgctg ttactttatt accctgttcc gttaactcat tcaaaattag ttgaaaatct
     4141 ggttccgcaa tagcctctgc gagcttctaa ccgctgattt ttcgggcatt tgcgttggtt
     4201 cggttatttc aaacgttttc ggctttaggc ataattttta ctgagtctgc ttatttagac
     4261 acattataaa taacatattc cattactgcc tcttgattgc tgccttatgc cttcctatct
     4321 ttgaaatggc aaagtcctta atacaacgga accatgggag atttcaacta tgagaagcta
     4381 tttcagcacg gcagggatgt gacaaagtac cgccgcatat ctgctgatca tgtcaggatg
     4441 accgaatgtt gcggcaggaa gattcttcac gttcaggctg aagctctcag gatactggca
     4501 cggcaggctt ttatcgatat caatttctat cttcatacgt cgcaccttga gaagctctcg
     4561 atgattctcc gtgatccgga ggccaccgac aatgaccgct ttgtggcatg gatgatgctt
     4621 agaaattctg tcgtttcggc ggaagggaag cttccgtggt gccaggacac aggcacagcc
     4681 attgtaatgg cctggaaggg tgaggatgtc tataccggaa tagatgatgc acttcatctc
     4741 agccgtggaa tatgggagac ctacaatgaa aaaaacctgc gctattcaca gatagttccc
     4801 tcttcgatgt tcgccgagaa gaacagcggc agcaatctgc cggcacagat tgacatcgcc
     4861 tcgtgtcagg gaggtgatta tcgtttcctc ttcatcgcca aaggcggcgg ttctgccaac
     4921 aagactttat tgtaccagga gtcgaattcg cttctgaacg aagcttcact cagcaggttc
     4981 atccgggaga agattgagga tcttggcacg gcggcctgtc cgccatatta tcttgccctt
     5041 gtcattggcg gcacctcggc cgagatgact ctcaagaccg tgaagctggc ctccaccggt
     5101 tatcttgata accttcccat ctcgggtaat tcttcaggcc gcgcatttcg tgacccggag
     5161 tgggaggaaa agattgtaaa gatctgtcag gaatatggtg tgggcgccca gtttggcgga
     5221 aagtattttg tgcgtgatgt cagggtcatc cgcctgcccc gccatgccgc ttcatgtccg
     5281 gttggcattg gtgtcagttg cagcgctgat cgcaatatca aagggaagat cactactgaa
     5341 ggcattttca tagagcagct cgagagagag cccgggaagt tccttcctga gaaggctccg
     5401 gagcttgaca aaccggtgga gatcaatctt gagcgcccaa tgagggagat actggctgag
     5461 ttgagcaaat acccggtaaa gacgcgcctg agtctcaccg gcaccctgat agtggcccgc
     5521 gatatagccc atgcacgcat caaacggatg ctcgatgagg gcaagccgat gcccggctac
     5581 ttcaaaaatc acccgatata ctatgcaggt ccggcaaaga caccggaagg gatggcatca
     5641 ggctcgttcg gacccacgac agccgaccgg atggatcctt acgtggacct cttccagagt
     5701 cttggcggtt caatgatcat gcttgccaag ggcaaccggt cgaagcaggt gactgattca
     5761 tgcaaaaagc atggcggctt ttacctcggt tctgtcggag gcccggctgc catactggct
     5821 gctgaaaaca ttgtctctgt taaggtggtc gacttcgcgg agcttggcat ggaggctgtc
     5881 cgcaaaattg tagtcaaaaa tatgccggct ttcatcctga cggatgacaa ggggaatgat
     5941 ttttattcag gcaatagcag gtgaacccca cccttccttt gtctgcgact cccgcaccac
     6001 ctcccgtgca gaaacctctg ttgaccagaa tgtaacggca ggtctgagac ctgccgttac
     6061 ctggtcataa cataattatt tcttcaatat ggtgtcaagg atcttctgct tgtcaagttt
     6121 tttggtgcag aagaaccagt cgtagcactt catctcatcc ttgttctcca tgcgctcttt
     6181 ggcaacccca catgatccgg ctgtgggaac gatgacatcg tcacccggct gccagtcggc
     6241 aggaagagcc actgagaatt tgtccgcagt ctgcaggccg atgactaccc tgtatatctc
     6301 ctcgaaatta cggccgaggc tcaacgggta gtaaaggatg gtgcggataa ttcccttggg
     6361 atcaataaag aagactgccc ttacggcttt ggtgtcactc tctccgggca tcatcatgcc
     6421 atatttccgg gcgacttcca tagttatatc ctcgatgagg gggaatttga cctccacatc
     6481 cttcatcccc ttgtactcaa tcttttcctt gatggtgcgc agccatgcta tatgactgta
     6541 aaggccgtct acggaaaggc cgacgagctt cgtatttgcc ttttcgaatt tttctgccat
     6601 tgtggcaaag gtcatgaact cagatgtaca taccggtgtg aagtctgccg ggtggctgaa
     6661 aaggattacc cagcttccct tatagtcatt ggggaaattg atctcaccct gtgtggttac
     6721 tgccctgaac tcaggagcct tgtcgccaat acgtggcatc gaataaactt tttcttccat
     6781 aattaattgt aatttagttg tttaataatt attttctttt tggtttcttc tctcagtcat
     6841 caaatcatac tcctccttca ttgcaacggg gttctcttca tggtcaaacc tcaccgtcag
     6901 ggtctgaagg atgatgttgc aggtttcttg cgtgaagttt cagaaattcg ttctgatcat
     6961 ccggggtctg aaggatgatg ttgcaggttt cttctttttc tttttcatga aatctggtca
     7021 tagtctctct tagagttttt gttgttccgg gaaaaatacc cgggcctgat aaatgccagg
     7081 gttgatatga taggagcctt atcatctctt cttcctggta ttcttcctgt tatcagtgta
     7141 atttccgacg atctgcagct tgatatcctc gattctgaaa tcgggaatac gattggtttc
     7201 aaaatagtca tggagcattt tcgtcagttc ggtgtcatag taatcatcta tgcggtcaga
     7261 gttggtgcag tagatatggt gatgactttc gtcagccgca tcatacctga gaaggccgct
     7321 gtcagttttt actcttttca gcagattctt ttcagccagt gtctccaggg ttttatagat
     7381 ggttcccagc gctatctcag ggtgtttttt actgatatag tggctgacct cctcggcggt
     7441 aggatggtta cgtagaacgt ctactgcctc ataaacagtg agtctctggg gtgtgaccct
     7501 cagtccgctc tccctgagcc tggttctata gttttccatt ttcatcacct aagaataatt
     7561 cctaacaaag aataaacaaa tataagctga aaaagttaaa taacaaaata cttcttttgc
     7621 tattttgttt aaagtatcac caagtcgctg taattcaaag gttcagatag acagaatttc
     7681 atttgccggc atatcaggca taatccggtc aacctttttc ctgttgccga ttgctcacac
     7741 aggggaacga atgaccctcc tgttcatgct ttaacatttt tgagaattgg ttgttatgat
     7801 taaagtcaga ctatcataaa ggcaatgtca agtatctgac ttatataatt atcgttattt
     7861 tagtggcctg aaaaaacgag agatgacaaa cagaaaatca ctggcacttg cagcgttgct
     7921 gctgatacag acagcgttcg tcaatgcaca ggttcttgaa ccggtggcat ggagtttcaa
     7981 aagtgaaaag atcgcggaga agagttatga tattgtcatg actgccacca ttgatgatac
     8041 atggcatctt tatgccatgg acctccccga gggtgggccg gtcgccacca gttttacctt
     8101 tgaaacccct gagggttata ccctggaagg caagccggtg gctgtcaata agccggaagt
     8161 caaattcgac aacagtttcg ggatggatat agggatgcac agtggcacag tagagttcag
     8221 gcagaaaata agcgtcaaca aacttccggt tactgtcagc ggtttcgtta cattcatgtc
     8281 ttgtgatgac acacagtgtc ttgctccgcg tgatctggag ttctcggtca atatcgggga
     8341 tgaagttgca gcatccgccg ataaagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnngatc tggagttctc ggtcaatatc ggggatgaag ttgcagcatc cgccgataaa
     8521 gttgatgagg ctgcaggcac caccctgccg gttgataaac cccgcaaggg ctttctgaaa
     8581 ttcttccttc tttcgatgct ggccggcttt gccgggatac tcaccccgtg cgtattcccg
     8641 atgataccga tgaccgtggc atttttctca cggggttccg ggaaaaagag ttctgcagta
     8701 gggcaggccc tgatcttcgg attatcaatc atattgctct attcatccct cggaataata
     8761 gtatcactga ccagcgccgg agcaggattt gccaacacgc tcagcacaca ctggattccc
     8821 aacctgctct ttttcagttt gtttttgata tttgccgctt cattcctggg cgcatttgag
     8881 atagttcttc cctcaaaatg ggtaagttct tctgacttaa aggtagacaa agggggagcg
     8941 cttgctgctt tcttcctcgg actgacaaca gtacttgtct cattctcatg caccggaccg
     9001 atagttggcg cactgctcgt agaggcagca acgggtgacg tccttcgtcc cacaataggg
     9061 atgttcgggt tcggacttgc ctttgccata ccctttaccc tgtttgccct cttcccctca
     9121 tggatgcaga agttgccaaa gtcaggcgga tggctcaact cggtcaaggt agtgctggga
     9181 ttcatcatgc tggccttcag cctcaagttc gcttctacaa tagataccgt ctataacctc
     9241 ggaatcctct ccagggatat ctacctggca atctggatcg tgctgttcgt gctgcttggc
     9301 atctatctga tgggtaaaat caggttcgtg catgacagtg acgttccgca tatcggtgtc
     9361 ttcaggctgt tgctcataat agcatcattc acctttgcgc tctgccttgt gcccggtctt
     9421 ttcggcgcac cgctgacgcg aatctctgct cttcttcccc ccagggagac atcaggattc
     9481 aacctgctca aggccatcag tgaaaacagg gctacggcgc ctgccaccga atttccgggc
     9541 atggtgccgg gtattgctgt agcaggcgct tcagggtttg acactccggc atcaggtgca
     9601 gtcagcgctg ccggaacagc cctctgtgag gaaccaaaat atggtgactt tcttcatttt
     9661 gaatatggtc tgcagggata ttttgacctg gatcagggcc tcgcatgcgc gaagaaactg
     9721 ggcaaaccgg tgctgatcga ttttaaggga catgcctgtg ccaactgcaa ggagatggat
     9781 gcgagggtat ggtctgatcc cgcagtgcag cagaggctgc gcaacaattt cattctggtg
     9841 gcgctgtatg ttgatgaccg gacaaagcta cctgagaacg aggtgtttgt ctcaaaagta
     9901 gacggaaagg aaaaaaagac aatgggcaag aagaacgagg atattgaaat ctcgatgttc
     9961 aacaccaatt cacttccgct ctatgctatc gttgacacgg acggcaagcc gctcatagag
    10021 tcccgcggaa ccgatttcga tgtccaggct tatatcgaat ggcttgatcg gggggcggag
    10081 gcattcagga atcaataacc ttattttgac atatagaaga caaggctgcc gccattcatg
    10141 atctcgtcat gggttatata gtggcggtca agcttcttgc cgttcaactc tgccttttta
    10201 acgtagatgt tatttggtcc ctggttcaca gccctcaccg taaacttctt tccgttttcc
    10261 agtgatatgg tggcctcctt taccagcggg ctgccgaggt catagcggtc tgagcccggc
    10321 gccaccgggt agaaacccag tgcactgaag atgtaccatg cgctcatctg cccgcagtcg
    10381 tcattaccgc tgagaccgtc tggcgccggc aggtacttgg tgtccattat atgtctcacc
    10441 cggtactgtg tctttcgcgg cgccccggcg tgattgtaca ggtagggtac atggtgcgag
    10501 ggttcattgc cgtggacata gttgcctata atgccctcgc gggtgatatc ctcggtttcc
    10561 gcgaagaagc tgtcagggag gtacatggtg aagagtgaat cgaggtgttc cacgaatctt
    10621 ttcttcccgc cgtggaggcg gatcagctct gccggatcat gnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn naagctctcc cgggttcagt ttgctgacag cctgtgctat
    10801 gcaccagtca tcataggcat actccagggt gacggagacc gatgatcccg tacgttcgtc
    10861 aggtacgtat cccagcttca tatagtcatc gaggccgtca taccagccgt tgcgggcagt
    10921 ggtgacacag gcggcggcag cccttgcatg gtcaaagcct ccgatctttt tcactatggc
    10981 atctgcgatt accggcaccg aatggtatcc gatcatgcac cagttctcat tggcatggtg
    11041 cgaccaaacc ggtagcatgt gatgaacgct ctgatcatag tgtgcgagca ttgagttgat
    11101 catgtcagcc gttcgttcct gctggatgat ggtgaagagc ggatgcagtg ccctgtaggt
    11161 atcccagagt gagaatgtag tatagttggt gaatccctcc gcatggtgga tgttctggtc
    11221 gagacccctg tagcttccgt cggtatcagt gtagatggtg ggagagagga aagtatggta
    11281 aagtgaagtg tagaaactgg tcatctgccc ttcggtcatc atttcaacct tcactttttc
    11341 cagctctttg cgccacaata cttcggcagc gagcctggca gcatcaaagt cagcacgtgg
    11401 cgcttcggca atcatgttgg ccattgcccc ggctgagctc accggcgaga tggctaccct
    11461 gacggtgatc tgctcaccgg gctctgtatt gaaatcaaag tgccccctga tatttcgtcc
    11521 ggccgcttcg ggaaaattgg tgcgctggtc aaagcggcgc cagaagcctt tgtaaaccct
    11581 ggctggtgag tcgtcacgga aaccgtatga tgtaaaagat ttggagaaag cgatggcaaa
    11641 atagagcgac ctggtgcggg cccatccgct ggtttgcctg aagccggtaa tcagtgtgtc
    11701 attttccaca cgtatgaatg tccagacatt tttacctgca tcattgtaga tcccatgaac
    11761 catgtcaagg atgaggtgtg catcgcctcc cttgttgaag gtgtaacggt gaacgccggt
    11821 acgtgtggtt gccgtcagct cggccagtat gtcatgatct tccaggagaa ctgaataata
    11881 accgggtgag gccttttcat tgctgtggga gaatcttgag cggtagccct tttctggcgc
    11941 atctgccgta ccgggattca gctgcagcgg tccggtggtt ggcatgagca gtatatcacc
    12001 gaggtcagaa tggccggtac cgctgaaatg ggtgtgtgag aatcctacga ttgttttgtc
    12061 agtatactgg taaccggcac agtacctgta aacatccctg ttgtaacttc cgttaatctc
    12121 gtagggaagt gtgtcagtat cgggtgagag ctgcaccatt ccgaacggca ccgtggcgcc
    12181 agggtaggtg tgacccatct tatcggtacc gataaaggga ttgacatatt tcagaatatc
    12241 tgcatcctga ccggcgcata gtattgttac catggaaagg agcagggcgg cgtgaatctt
    12301 tatgctcatt gcttgtacag ttgctgaccc aaaggtaaaa atatcgtcag caaaaaaaag
    12361 ctattacttt ccggaaccag cttctgttgt catcgctgtt cctggtattt cagaagctcc
    12421 tcgttatatt tcctcctctc ggggatgcgc acatatctga gcccatgctt gttgccatag
    12481 tcagaacagg ccttctgtgc ccatgatgtt acagcctcca ggttgcttaa gtcattaccc
    12541 ttcctgcaac ctggctgctt cgctaaaatg atccgacgaa tctttatttg acgtttgtca
    12601 ccctattcga ggcttcccac ggtcttctcc acctcatgca gaatctcgca tgatttcacc
    12661 agttccttca tggtggtgtg gtcttcatag agcggacgat cgatatccag gaactgcacg
    12721 tgtttgcgga tgacatcttt ggctttctgt acccctgttc cgaagctgta ctcgctcttc
    12781 ctgaaatcaa gagcctgtgc tgctgccata tattcgatgc caagaatgcc gtaagcgttg
    12841 tcaagtatct ggttgttctt gatggctgtg ttcatgccca ttgagacaaa atcttcctgg
    12901 tcagcggcag ccggtattga ttgtattgat gcgggtgctg agagtatcct ctgttccaca
    12961 atcagcatat cagcagtata ctggctgagc atgagtcctg agaacatacc ggcacctttt
    13021 gtaaggaatg caggcaggcc gacgttgaga gcgggattgt taagcctgtt cagtctcctc
    13081 tctgacaaca cgcacaccat ggttattgtt gcgcctgcca tatccattgg aacggcaacg
    13141 ggtgttccct ggaagttagc gccggagagc tgcaggtctt tgtcagggaa gaataccggg
    13201 ttgtcacaaa cgccgttgag ttctatctca acctggctgc gggcatatcc cagcatatca
    13261 tgtgcagcgc cgataacctg cggggttgat ctcattgagt aggcgtcctg tacctttgac
    13321 ttgatgcgcc cctccttcag gtcaccgccc gtaacacatt tattgattgc atttgcgctg
    13381 cgcatggctc ctttgaaacc gcgtacctca tgaagcagcg gagtgtatgg cttcatgttt
    13441 gccttgagag cctcgagcga cattgcagca gctatctcag cctgtttcag ccagttattg
    13501 gcatcaacca gccagatggc gctcatggcc gtaagcatat tcgaaccgtt gattactgcc
    13561 agtccgtcac gtaccagcag tccgggcacc ttgatacccg cccttttcat ggcttcggcg
    13621 ccgctgagaa gctctccctt gtaaaacgcc ttcccttcgc ccatgagaag gagtgctatc
    13681 tgtgacattg gtgccaggtc accgcaggct ccaacggatc ctttctggca agcatatggt
    13741 gtgacaccct tgttaagcat ctcaacgtag gtctcagtga tctcaagacg gcagcccgaa
    13801 ttgccgtggg catgcacatt tatgcgactg agcatcgcac cgcggacaat ctcaatcggg
    13861 gccggatcac caataccggc agcatggtta tatacgagat atttctggaa ctctttgatc
    13921 tgctcatcgt tgagaacaat ctcggagaac tcgccaatgc cggtgttaac accgtacatg
    13981 atctcatttg cggcgatttt tttctcaagc atggcgcggc attcgagaat cctcttgcgg
    14041 gcatcgggat gaagctccac tttctgtccg tgtcgtgcaa cattgacaac gtcattgacg
    14101 gtgagtcctg aacctgtaat tattaatgta tccattgata ttaagttttt atgttttctg
    14161 aattgatgat ttataactgg tcaagaaaaa gcgaagagaa gcgacttcac cgtggtgcgt
    14221 cgccttgcgg gcggcattca gtacatgccc gagcggttga ttttgatctt gtattccagt
    14281 acccgtctgc gtgtcatgat gcagatttac agcaccaaat atatgaaaag aaatgaatct
    14341 gccatgtgac gtaagttaca gccactcttt tttgctatca tatatatcat aattctgcaa
    14401 tagtccctct ttttttgtaa tctttaggtt ttaaatctct gatatatgaa aagcagattt
    14461 accataattt tgctgctggt aattgttcct gcagcaatac tcctttcact ggcagccttt
    14521 aacataatca gatcgcttcc gcaggaagat ctgaagggca ggtaccagta caaggatttc
    14581 gagtcggccc tcaagtgccg ctcgtgtcac ccgggcatct atgagcaatg gtcccaggcg
    14641 atgatgtcac aggcctacac ccatcactgg gatgagattg agtattttga gcttgccgta
    14701 cctcatgcgg aggttaatcc ttccctgaaa gacgcggttg acggctgcaa tggatgccat
    14761 acaccgatgg ggtacatggc aggggtattc cctccgcccc gtccatcgga aaagagcatg
    14821 gcaaacgagt cagtctcgtg cgaggtctgc catcttaccc ggagggcaca ggttgatccg
    14881 cctttcaatt tcagctacta catcgaaccc gggcgggtaa aatacagcgg tcgggagccg
    14941 gaaatacagt cgcctgcaca caagatcgtt cagaatgatt tcttccggac cacggaattt
    15001 tgcggcaact gtcacaacga gaagaatcct tacggcatct gggtgaaaag cacgcagctg
    15061 gaatggatgg aggggccata cgccagggag ggtgtgcggt gccaggactg ccacatgccg
    15121 gagggacagt acacccttgc tctgatgggg aaggagcgtg atgatgcccg cctccacctg
    15181 ttccacggag ctcatgaccc aggcaaggta agaggaacca ttgagcttcg gattgagcct
    15241 gacgtgcgtc tcgctgagcc cggagagaac gtagtcttta ccgttgcact cttcaatcag
    15301 aaaaccggcc acaagtttcc caccggctcg gtggaagacc gaatagcctg gcttgatgtg
    15361 gaagctactg acgcaaaggg taaaacatat cacctggagg ttgacaggaa aggtttcgaa
    15421 ggcgaggagt acactatctc gggtgattat cttgcctacc aggatatggc catgccgctg
    15481 aagcttaatg atttcaaagg ggtgcagcgc gacggcatac catacgggaa caggatattc
    15541 cggatgccct ttttcgacga gcaggggaat atgacaatcc tgcagtggaa caccttttcg
    15601 ctgggagttg attaccgcat tggccccagg gagacaaagg tggagaaatt cacattcaaa
    15661 ataccctggg atgctgcacc cggtgaaatg aaggtaagag ctgtgctcaa ctaccagctc
    15721 ctcgtcaagc cggtagccga ctttcttaaa gtaccaccgg atgagtcaga gatcataatg
    15781 gtcaatgagc attcaacaac agtcgggatt cttccctgat aatcaaatct taaagaaatt
    15841 tatatgaaaa cttctttcat tgccagagct gccatactga ccgtaacgat cattatgctg
    15901 cttccatata agcagatgtc ggccataccg gcattcgcac gcaaatacca gatcagctgc
    15961 caggtgtgcc atacacctgt catgccgaga ctgaaagcct tcggagacga ctttgccggt
    16021 gacggtttca ggatgacgga gtatgaatca ccacggcatt tcatccagac aggggacgac
    16081 cggttatcgc ttttccgtga gctgcccctg gcattcagga ttgatggcca cgtgactgca
    16141 aacttcaacg gtgagggcag cgttgatttt gctgctccgt ttatgcttaa gatcctttca
    16201 gggggtgagt tgtcagacag actctcctat tatttttatt tcatgcttga tgagcgcggg
    16261 agggttgccg ggctggagga tgccttcatc atgtataaag accttttcag aacagggata
    16321 aatatatacc tgggacagtt tgcagtctca gacccgatgt tcaagggcga gatgcggtac
    16381 actattgaaa actatcacat ctatgctaca gcgccgggtc agtcatctgt taacctgaag
    16441 tatgacaagg ggatacttct cgacaagggt tttaaaacag gtaccacggt ggtgtttgag
    16501 gtgataagcg gaacgggcct tggcagtgcc gatcactggt atgttttcga caaggacaag
    16561 tacaagaata tgctgctgaa agtcacacag gatatcggtg agtatgccag cctgggtttt
    16621 ttcggttaca ccgggaagga gcttttgtgg accggtactg gtgatatcgt gagctcggtg
    16681 aagatgtacg gccctgatct ggtgctcgat ttcaacgaaa agctgattat cgggctgcag
    16741 tatctgtgga ggacggattc ggatgtttac ctcaatccgg ggcagccggt gagaaagaat
    16801 gtgcttaccc atggcggatt cgctgaggtg atctattccc ccaaagggga tatgagtacc
    16861 tggtatctta acgggctgct caactgggtg gaatcagacc tggatatgct tgattatcgt
    16921 tctgcaaccc tgcatgccgg atacatcatc agacgcaacg tgagactggt aaccgaatat
    16981 accagggtct tcagcggcaa tccctacggc aagctcagcg gtggctttgt ctcagcattt
    17041 tgatgcacaa gttgtgcagc aaccgtgaaa ttttcgagtc ttttatttaa taatacttta
    17101 aaatcgatcc tatgaaaaaa ttcatggttt ttgccgctat tctatttctc gcttcctgta
    17161 ccgggaagaa ggaggtgaag cagtacacta tcgaccagtt ctacaaaaac gtaagcattc
    17221 agttttcaga cttctcgcct gatgagacca aaggcctggt aaccagcaat gagaccggga
    17281 tatacaacct gtttgagatt gactttgctg acggtaccag gaagcaggtt accttctccg
    17341 acaccgaatc actgtttggc attggttatg tccacggcac cggccagata ctatattcat
    17401 cagacaaggg cgggaatgag aacgatcata tttatctttt aaatgaggac ggaaccagca
    17461 ccgacctgac tcccggtgaa gccgttaagg catcattcag cggcttcagc cttgacaaga
    17521 aggtgatcta tcttctttcc aacaagcgtg atgccaggtt tttcgacctt tacaggatga
    17581 atgtgggtga atggaaggag gagatggtgt gggaaaacag catggggctc gaatatgccg
    17641 gagccagcag ggacagaagc atcatagcga tggtaaaacc gattactacc agcgagtcgc
    17701 agctttttat ccatgacctg gccacggggg agacgaaaga ggtttccgaa cccggcaagc
    17761 ccggaatgta tatggcttcc ggctttacga atgacaatgc gtggttcttc tatactaccg
    17821 atgcaggcag ggagttttca tacctcatga ggtactccct ggctaccggt gagcgtgagc
    17881 ttctctatga aaccagctgg gatgtcaccg gcagctggct gagcaggaat gaaaaatacc
    17941 agctcatagg aatcaacgag gatggccgga tacaggtaaa catcactgac tatgcgacag
    18001 gggagaaggt tacccatccg gaggtccccg gagccagcgt caacgagctc gcaatctcag
    18061 agagcgagaa gtacctgttg ctcagtcttg gcacatccaa aacaccgggt gacctctatc
    18121 tgtatgagtt tggaagtacc gggataaaga agctgacaga cgtcctcaac ccggagatca
    18181 acccggctga ccttgtcgat gcgcaggtgg tgcggttctc ctcgtttgac ggcctggaga
    18241 tccctgccat ctactacaag ccactgacgg caacaaagaa aaacaaggtt ccggcactgg
    18301 tatgggtgca tggcggaccg ggcggacaga caatggcata ctataatccg atcatacaat
    18361 atcttgtaaa ccatggatat gcagtgctcg gagtgaacaa ccgcggtagc agcgggtatg
    18421 gaaagagctt ctacaggatg gatgaccgga accacggtga caaggacctg aaagactgca
    18481 tctggggcaa gaagtggttg caggcacagg aatatatcga cggtgaaaag atcggcatca
    18541 tcggtggaag ctacggcgga ttcatgacca tggcagccat gacatcggct cccgaagaat
    18601 tcagggttgg cgtcaatctc ttcggggtga ccaactggtt gcgcacccta aagtcaatac
    18661 cgccttactg ggcatcattc cgtgatgctc tctataccga gatgggtgat cccttcacgg
    18721 ctgactcggt aaggctctat aatatttcac cgcttttcca cgggcagaat gtaaagaacc
    18781 ctgttatggt gcttcagggg gccaatgacc cgagggtact gcaggtagag tctgacgaga
    18841 tggttgaggc tatcagggct gccggtgtgc cggtggagta tgtgatcttc cctgacgaag
    18901 gccacggctt tatcaaaaag gaaaatgaaa tcaaggcata ttcacagata cttcaattcc
    18961 ttgacaaata cctgaagggt gccggggagg agggctgacg tcatgcagac cccctgaacg
    19021 tgatatgttc caatgaaaac aggcaaatgg ccggcggtca ggcaagatcc ccggcctttg
    19081 tttttgactg tagtgtaccc tgagttaaag atgatccccg gctttcactc tcagcctgcc
    19141 ggagattaga ttcctgttca cagatcacct gcttcctgcc gtttgccggt gaacggttca
    19201 atggcgcggt cgaaaatacc gtgaaggtaa gcaatggcca ttccgtagtt ggtaatggca
    19261 acattatgtt tttttggctc gcgcaacctg ttaaccagct gccggtgcgt caccatgcat
    19321 ccgccacact ggataaccag ggcataatcc tctatcggcc gcggaaaggt gtcaagccct
    19381 gcaacgacat caaaatcaag cttctttcct gtaaagttcg tcagccagcg gggaatcttg
    19441 actctcccga tatcttcaca ggagacgttg tgggtgcatg attccagcag caacaccctg
    19501 tcaccgtcct gcaactcccc gatcttcggc gttccgcgaa ggtactcttc gaagtcgccc
    19561 ttctgcctgg ccagcacgat actgaaacct gtcagcggga tatcacgggg gataactgca
    19621 ttaacctttc cgaatacctg gctgtcagtt accaccagct gcggcctgat gccggtcttt
    19681 tcaaggaatg actctatatc agtctccttg aggatgattg ccgtacagtc attgtcaagg
    19741 gtatctctta tgacctgcac ctgcggaaga atcatcctcc cttcaggcgc ttcaatatca
    19801 acaggtgtca caaggaggac tatgtcgccg ggattcacga ggtcacctat aaggccatgg
    19861 ctcttcattg caatcggggg catggcctct tttatcattt ccaggagggc aggattgtac
    19921 ctgtcaggga acaatgccga gtaagcggca acctccctgc cggtgagatt cctgattttt
    19981 ttgcgaaact catccttaac cggagagagg tcacttttgt tatgtacaat cacataagga
    20041 atatcgagtc tgccgaactc ctgcgtaagt tcggcttcat ggtcgtcaaa gctgttgtca
    20101 gaaagcacca ggatagccag atcaataagc ttcagggcat ccattgtttt ttttatcctg
    20161 agctctccca actctcccga atcatcaatt cccgccgtgt caatgagaat aagcggcccg
    20221 aggccatgga tctcgatact ctttttcacc gggtcagttg ttgtgccggc tatttcagag
    20281 actatggcaa tatcctgacc aacaagcgca tttatcagtg agctcttgcc cacattcctc
    20341 cgtccgaata tacctacatg gggcttggta tctcttcctt ttgctttcat aattcgctct
    20401 cagttccttt gacttttctc ccttgaattc ctttccgcgg cacaggtttt ttcatgattc
    20461 atcgttggaa acgtgagggg agagtttcct cgactgcgct tccgcatcga tttcttcact
    20521 ctttttctgc agttcgtcta tcgagatgat cttggcgcgt ctttcagtcg gtggattcag
    20581 cttttccgac ttttcgaact cgatgacctg cttcctgaga tcttcaggca gttctttctg
    20641 gcttctcagg acgatatcaa tgaattcttc agaaaaaaac tggtcaacct gtatccgtcg
    20701 cttggccgtg tgggcgaact tgccgatgat acggcagata ctgtcacggt cctccgggct
    20761 cagtttctcc gccaggtcat actcctcgat ctttttaagc gacagtatca ccggctccag
    20821 ggcatccttt gtgatcacga atacgtcata gtcacccagc cggtatgtca gctgcctgat
    20881 actgtcgatg gtattggtcg gcacaaccag gaatatcgat ttcctgacat tatcctggct
    20941 ggcgtatttc ttaaggtttt cagcctgggt cttgatgtag ttcgggaagt tggtgagatc
    21001 gtcgcctgcc gggcttttgg cgtcaaagat gatatactcg ccgctgatct cgattgtgtt
    21061 gtcaggattt cccctgaagg ggaccttttc cacgtagtta acgtggttga catcgcagat
    21121 gcgtatgatg gagtttttca cgtctgcttc gtgctctgac cattgcttct tcatggcttc
    21181 cagcctttca gacgtctcgc g
//
LOCUS       DAOPBK010000019        20988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2647_length_20988_cov_4.817131, whole genome shotgun sequence.
ACCESSION   DAOPBK010000019 DAOPBK010000000
VERSION     DAOPBK010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20988
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2647_length_20988_cov_4.817131"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLA17_02080"
     CDS             <1..630
                     /locus_tag="PLA17_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HPK84231.1"
                     /translation="ALDISRGFHAIMQEVTESGFSRIPVYSETLDHIRGILFVKDLLP
                     YLGKPDNFRWQSLIRPAYVVPETKKISELLKEFQTKKIHMAIVVDEYGGTSGLVTLED
                     VLEEIVGEITDETDEEVPLYKQTGEGKWVFDGKIMLNDFYRITGLENDPFQEVRGDSD
                     TLAGLLLELLGEIPVKGREITAGGFRFTIESIENRRIREITAEKKKEEI"
     gene            627..1199
                     /gene="gldD"
                     /locus_tag="PLA17_02085"
     CDS             627..1199
                     /gene="gldD"
                     /locus_tag="PLA17_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HPK84232.1"
                     /translation="MRKTMAAALLASALIFAGCDHPTVPKPKGYFRIDLPQKSYVHFD
                     SPCSYSIEYPEYAVINLHPMAATDTCWMDIEFPSLKAKIHLTYFDINDNLASLTELSR
                     EMAYKHTPKAEAIDEKLWTDESAGVYGMLYDLKGNTASAVQFFVTDSIRHYLRGSLYF
                     TAQPNEDSLMPVISFIREDIIHLIETLNWR"
     gene            1203..1847
                     /locus_tag="PLA17_02090"
     CDS             1203..1847
                     /locus_tag="PLA17_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPK84233.1"
                     /translation="MGCITKHYLNRDTVIGVWKIEEDIDTLLSMVDFSPEDRKRYEYF
                     RSNSRKTEFLSVRALLAEMLGREARIVYNKNNKPFIKDGSHFISITHSNKLTAILLSK
                     TERVGIDLEYMRMNINAFASKFINKGEKITRRWADRTYHLYIHWCAKEAIYKICDKEG
                     INIVNDITIEPFVVKESGEIRGSVENERISDQFTLYYSRYDNYTIVWTKKKHDG"
     gene            1837..2577
                     /locus_tag="PLA17_02095"
     CDS             1837..2577
                     /locus_tag="PLA17_02095"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPK84234.1"
                     /translation="MMVDIPWKKRKLIARLIDPDHAGEETLVTAVRLADEAGVDIFMA
                     GGSLTSVPVTDVIRKLKQLSSIPVVLFPGNLTQLTAGADAIFLLSLISGRNPELLIGS
                     HVIAAPLLKNMRERVIPVGYMLIDCGGGTSVEYMSQTSPIPSGKNDIAVATALAGEML
                     GMKAIYLEGGSGAARPVNPSMIKAVSDELTIPVITGGGIDSAEKVEVAFGAGADMVVI
                     GNGCEKNPELIKGACRVRDSYNRDTFII"
     gene            complement(2606..4255)
                     /locus_tag="PLA17_02100"
     CDS             complement(2606..4255)
                     /locus_tag="PLA17_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPK84235.1"
                     /translation="MKRVITVLYLLVLTVSVTAQSAYIPPDKPKLVIGIIVEQMRYDQ
                     LERIWEILPDNGLKKMVNEGTFYRNASIDYLSTQAAPGFATISTGASPSAHGITSDSW
                     FHPFNDEMIFCVQDASASPVGGSFETGLFSPVNLLSSTFADELQMASCGGSKVYGIGM
                     KEMSAIITAGHAADGAFWYDDRTGTWMSSTYYTDSLPPWLMDFNAMMMPQQLLNQQWL
                     PLLDPATYPGCQPDSSSLERGFNGQTWFPYNLKELSTKGKLLNVTRDFSVLRQTPFAD
                     DYTTELALRLIDNEQLGQDDITDFLAITYSSTDYVGHRFGPSSVEASDALVRLDRNIA
                     RLIENIDKSLGKKNVLIYLVSAHGVSEIPAVLEQSRVPAGYFRLNQSLQLLRSYLNAV
                     YGQGDWVRGFFDSQIYLNRGLIEGAKINLEDMQKKVARFMVQLSGISAAVPTSAFETS
                     DFSGGLLRKMSNNYNQLRSGDVMIALNPGWVEKTDDVTGHNSPWEYDNHVPLIWFGWT
                     ASRASVTRSVSTRDIAITLSVLCKVPLPNASSGDPLHELFR"
     gene            complement(4343..6058)
                     /locus_tag="PLA17_02105"
     CDS             complement(4343..6058)
                     /locus_tag="PLA17_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPK84236.1"
                     /translation="MKLHSIIPVLLLLVSMQAVCGQESPAGDDIRNVVSRYGQAEVMI
                     SYPGYDAMTRLAARFSVSSCDGLTATLCLSPRDVDEFIAAGIPCKLIAPEERKGFHTA
                     SSVAEAMRWQSYPTWKHYDTIMHKIAGQWPDFCRLDTIGFSVQGRAVLVLKISDNPEE
                     DEPEPEVMISSSIHGDETAGFVLLMRLAEYLASSGGAAGAGLASELVSELEIWINPLA
                     NPDGMYRGGDTLIYPVRANSNGYDLNRNFPDPEVAAPPPLQKETLDMIRFMEERRFAL
                     SVNLHSGAEVVNYPWDKWTRNHADDAWFNAISRRYADTVHLWSEPGYMTLLDNGITRG
                     SNWYQIRGGRQDFVTWGLGGREVTLELDDTKMTPGSNLELMWEWNHRSLLRYIAEALT
                     GIKGMVTDAETGDPLSAKIFITGHDRDSSHVWSDTTGFYTRLLPPGTWNLTFSCPGYK
                     TYVLTAELTSSSPLQVSDIQLEKDNQDYPQPPESGLLVFPNPSDGNFNIMPPVAVTGE
                     VTVTVTSTSGALMKHYRTTARPGIPVECRFTGLPSGLFIVSVRREPNGPMVRGKAVIT
                     QLINR"
     gene            complement(6067..7155)
                     /gene="ychF"
                     /locus_tag="PLA17_02110"
     CDS             complement(6067..7155)
                     /gene="ychF"
                     /locus_tag="PLA17_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPK84237.1"
                     /translation="MALKCGIIGITSVGKTTIFNCISNTRGETDTYGTGVKANLGVIK
                     VPDKRLYELEKFQPTERIVHTTVDIVDIPGLVRGQGDSSGARLLGEIRNTDALIHVLR
                     CFDDDNLPHIDGSVNPVRDLENVEFELQVRDVESVMKKIQRLERAAKTGDKEAKHGIE
                     ILNLYREHLEGFQNASSLELKPEEKKVVDDIFLLTMKPVLYVCNVDEASAVDGNRYVD
                     DVKSFLAPRGAEPLMIAGALEAEIANLTEETDRLEFLEAAGLTEPGVNKLVGAAYRML
                     NLQTFFTVGPKEIRAWTITAGMTAPQAAGVIHSDLERGFIRAEVMKYDDFIRLGSEHA
                     CREAGKLMVEGKTYVVNDGDILHIRFNV"
     gene            7266..7829
                     /locus_tag="PLA17_02115"
     CDS             7266..7829
                     /locus_tag="PLA17_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK84238.1"
                     /translation="MPQITEENIRITEDNIREALATDADKAFRMIIDLYGRRLYWHIR
                     RLVITHDDADDALQNTFLNAWKNIGSFRFDSSLLTWLWTIATNEALTLIRRRAREKSV
                     AFDDVSEILAASAEGSTWFDGDAAMVRLQNAILKLPEKQRAVFNMRYFDEMSYEDMSS
                     VTGTSVGALKASYHHAVKKIEQFLERD"
     gene            8052..8591
                     /locus_tag="PLA17_02120"
     CDS             8052..8591
                     /locus_tag="PLA17_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84239.1"
                     /translation="MKLNDEILKNNPFRVPDDYFDTLADRTMAAIDKEAGKDGKRAVI
                     NMKAGKDGKRAMINMEAARDGERAAISEENIRKPGRVIRLKPFMALAAAILGFAILAT
                     ALVRLINADRPGMVTDSGHSLYADLIVEEIDTYMLEDELHMTDPAIPEMTMETIESEA
                     IIDYLMTEDITLDDIYELL"
     gene            8599..9138
                     /locus_tag="PLA17_02125"
     CDS             8599..9138
                     /locus_tag="PLA17_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84240.1"
                     /translation="MKRIITTIAALLLTGVAAMTLRAQGQGMGQGAMQGDSEKLTAYK
                     IAFFTKNLDLTPAEAEKFWPVYNDYSAKKSKLQTDRMLLLRNAAHHQASMTENEVASS
                     ADKLIKSFADEAALVASFYESLKKVLPPAKVIRLCQVENQYKQHLLRELNQRRQGQSQ
                     KGQGLRRSSGQAGSAALTE"
     assembly_gap    9248..9347
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9378..10766)
                     /gene="hisS"
                     /locus_tag="PLA17_02130"
     CDS             complement(9378..10766)
                     /gene="hisS"
                     /locus_tag="PLA17_02130"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPK84241.1"
                     /translation="MAQKPSIPKGTRDFSPAEMLCRNYIFDTIRKIFSLYGYLPIETP
                     AMENLSTLLGKYGEEGDRLIFRILNSGNFLADVSPSALSAADTSAVTRHISEKGLRYD
                     LTVPFARYVVQHRDEITFPFRRYQIQPVWRADKPQRGRYREFFQCDVDVIGSDSLLNE
                     DELVRITDRIFGELGINIIIKINNRKILAGIAEIAGAGERLTDITVAIDKIEKIGREG
                     VRQELAAKGIAPGVIARLEPIIDLSGSTEQKLAVLKKAMAESASGMQGIREMEELTGY
                     LAVEPVKATVELDLSLARGLNYYTGTIIEVSAADLPFASICGGGRYDDLTGIFGMPGV
                     SGVGVSFGADRIYDVMMQLNLLPEQTGSSTEVLVINFGSAELPALMKVAGELREAGIA
                     TELYPDPAKLRKQFAYADSCMIPLVVIAGSNELAAGQATVKNMKTGEQTAVPFHELTS
                     FIRRLKTLLHTR"
     gene            complement(10979..11335)
                     /locus_tag="PLA17_02135"
     CDS             complement(10979..11335)
                     /locus_tag="PLA17_02135"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPK84242.1"
                     /translation="MDEIKTYTQSERDWAMFCHLSSFAGYFFPFGGIIGPLICWISRK
                     DQSEWVNTNGKSALNFQISILLYTILLIPLCFIIVGIPLLVALGILEMVCVIVASIKA
                     AQGEVFKYPISIPFIQ"
     gene            complement(11381..12232)
                     /locus_tag="PLA17_02140"
     CDS             complement(11381..12232)
                     /locus_tag="PLA17_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84243.1"
                     /translation="MKKISTLALSLLLASAIFAQKRPYPTFDDAEKFKKSVTCIVRED
                     DPFSFYNAEIKAAVDKYWKATPVKYITTEEFNVMRNDPAYSFLVLTISNFSNDKSGST
                     YDFLNLLLGADVDELDQLPEFCAIPLCFSGAPEEEYSYKLGLIIRFMEYHADLVMKNP
                     TLSALRYLKYYNKNVPEIKDKTILVRQSDLAPEIDTPEKIASYYPYKVRIVDEEEIIQ
                     AIEEKQKDVLIVHKVGPDGVKQTGTCMKTLIGTDDAIMYYYDDHLVDRRNASGLLISD
                     LKRLARF"
     gene            complement(12243..12992)
                     /locus_tag="PLA17_02145"
     CDS             complement(12243..12992)
                     /locus_tag="PLA17_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84244.1"
                     /translation="MKKCLLTLIIALVATGLAKGQQGDTLVQYTPDFKFRDGIYLNFD
                     MVKTNSPIPKAKLFTSVDYNDKDFFDQVISSEKIYFYDNMGVRQEIEKDAIWGFARNG
                     VLYAKVQNNFNRITFFGSIIHFVADVTTQTGYNPYGYYDPYFYSPYRSFYSPYYYNPY
                     YSRYYDPYYYGRSPYGRHTSRTDLEMFMIDFETGKTYEYNLDNLEALLMRDPELYEEF
                     VSMRNKNQKKMMFVYLRKFNERNPVFLPSGN"
     gene            13133..14464
                     /locus_tag="PLA17_02150"
     CDS             13133..14464
                     /locus_tag="PLA17_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK84245.1"
                     /translation="MPNRSRSSTIPHSDLMNRWTRDRILLPVAVALLLVVIALVTALA
                     GLVYVPIAAGVLALAPLVLAIAKLRKLIADNMAIFRELALIAEKNEEVINSFSHKIRE
                     PLNNLVLLGSMLSETTTSPKQQELVDTFIASTGSMVEVVNELTMETAGNITFEKRGEI
                     CFRLGPIIRDTIGLMKLSRRHEQSVVVYDDRGEGETEYRGDPIIIKQILIDLLSTAIS
                     GPSEKVTVKIQVRDAGERDGVHKLEFTLSSNNQIVFIGKDRQPYPLAGRLIRDCGGKW
                     QEATSGTGSYFVFYIFLRRAEKVARPAAASSHIREMETEKSRKELKELNILLVEDNPI
                     NQRITQLMLEPLVKRLEVARNGKEALDMFGTARYDLILMDVQMPVMNGLVAAEKIRGL
                     EMSSSGHVPIIAITANAMLGDREQCLAVGMDDYISKPIEPEVLIEKITALI"
     gene            complement(14467..15399)
                     /locus_tag="PLA17_02155"
     CDS             complement(14467..15399)
                     /locus_tag="PLA17_02155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPK84246.1"
                     /translation="MRDSTALNSLIVFLIIAALVYLIILQSWVLLRTKKHLREIQGAY
                     GEIEAQRSELQLRNKELTDSMNYARRIQAALLPAEHHIRRIFPDHFIYYRPKHIVSGD
                     FYWISERDEKYFIAAADCTGHGVPGALMSMIGLELIQKIVNELKVDDTDQVLMTMNRE
                     LESAFFKEESGKALIKDGIEMSICIIDKNTKVMEFSGAFLPVYIVRDDKLIEIKGDKK
                     NVVQSFAMVSFSRSTFTLQEGDILYLFSDGYADQFGGPENKKFMYRRLRHILLTISKY
                     PLTDQQRILDETIVSWMEGHDQIDDIMILGVKPF"
     gene            complement(15444..17333)
                     /locus_tag="PLA17_02160"
     CDS             complement(15444..17333)
                     /locus_tag="PLA17_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HPK84247.1"
                     /translation="MSFNGLSQFVSFLESRGELVRVTSPVAPVLEITEIADRLVKNGG
                     KAVLFENTGTDFPVLINTFASDTRMAAAIGRNSLDDAGDELFALVESLTRAQGLTGKL
                     ARLPGMLRLLTLSPKRKSGRGECQEVVEQTPDLDRLPILKCWPHDRERFITLPLVHTR
                     NPETGKYNLGMYRMQVMGRDRTGMHWHRHKTGARHFEAWKRRGGRMPVTVTLGGDPVY
                     TYVATAPLPEDINEYLLAAFLRRKRVTLVKCLTNDLWVPADSDFVIEGYVDTAEAPVT
                     EGPFGDHTGFYSLADLYPMFHVTCITHRKNAVYPATIVGVPPMEDAWIGKATEKIFFA
                     PIKIAIAPEIRELHMPASGVAHNLVIVRIEKSYPGQGMKVLSALFGAGQMMLSKYIIV
                     VSGNVHVTDYLAVAEEVFANVRFDSDLLLTRGPLDVLDHSSDRVSMGGKLGIDATLKL
                     PEERIDEYHSIPLLLLTGWQFDEKVITGMKTLADYALPVAILTITKPEEGLDMHLLAA
                     SLPASLTTPGTVIIAVDDGADADDPAMLTWLVTGNSDPARDLYHLEGGAIFIDATSKT
                     RCLPVFPRDWPNVVCSDPETIELVNSRWPEYGLGPFIESPSLRLLPLLMPGEASVKTV
                     NSTAG"
     gene            complement(17437..18714)
                     /gene="eno"
                     /locus_tag="PLA17_02165"
     CDS             complement(17437..18714)
                     /gene="eno"
                     /locus_tag="PLA17_02165"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPK84248.1"
                     /translation="MSQIVSIHAREILDSRGNPTIEVEVITTSGASGRAAVPSGASTG
                     ENEALELRDGDKSRYLGKGVLKAVHNVNNVIAEELMGMDVTNQIVIDQKMIDLDGTPT
                     KSTLGANATLGVSLACAKAAAAYHGLPFYRYIGGTNAHVLPIPMMNIINGGAHSDAPI
                     AFQEFMIRPKGAGTFAEGLRMGAEVFHSLKKVLKERGLSTAVGDEGGFAPALKGTEDA
                     LDCIIKAITAAGYKPAVDVMVALDCAASSFFENGIYDYTKFEGKDAKKLTPAQQADYL
                     TALVEKYPIIDSIEDGMSEGDWEGWKMHTDQLGSKIQIVGDDLFVTNVKYLEKGIRLG
                     CANSILIKLNQIGTLSETLSCIEMAHRAGYTTVISHRSGETEDYTIADVSVAINSGQI
                     KTGSLCRTDRVAKYNQLLRIEEELGDNAVYGYR"
     gene            complement(18826..19434)
                     /gene="rplQ"
                     /locus_tag="PLA17_02170"
     CDS             complement(18826..19434)
                     /gene="rplQ"
                     /locus_tag="PLA17_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPK84249.1"
                     /translation="MRHLNAINSLGRKSADRKAMMANLASSLILHKRISTTTAKARAL
                     RTYVEPLITRSKDDSTHSRRVVFSYLGNKNAVAELFREVAPKIASRPGGYTRILKTGS
                     RIGDAAEMCIIELVDFNENMLAAGEGTKTRASRRRRGSRKKSAEVAPAAETAVTEAAT
                     ETIVAEAPAEAAAATETTAEVAATEAETPAEETGAETEDSKE"
     gene            complement(19457..20449)
                     /locus_tag="PLA17_02175"
     CDS             complement(19457..20449)
                     /locus_tag="PLA17_02175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPK84250.1"
                     /translation="MAILAFQKPDKVILLEADDQYGKFEFRPLEPGYGITVGNALRRI
                     LLSSLEGFAITTVKIEGVDHEFSTIPGVMEDVTEIILNMKQIRFRRMVEDIDNEKIVV
                     KVSGQEAFKAGDLNNSLSSFEVLNPDLVICRMEPDVKLQIEINISKGRGYVPAEENQP
                     AEAEFGLIAIDSIFTPIRNVKYAIENYRVEQKTDYEKLIIEINTDGSIHPKDALKEAA
                     KILIYHFMLFSEEKITIDYEDRMANEEFDEEILHMRQLLKTKLVDLDLSVRALNCLKA
                     AEVEALGDLVKFNKNDLLKFRNFGKKSLTELEDLLATMGLSFGMDVTKYKLDKD"
     gene            complement(20470..>20988)
                     /gene="rpsD"
                     /locus_tag="PLA17_02180"
     CDS             complement(20470..>20988)
                     /gene="rpsD"
                     /locus_tag="PLA17_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPK84251.1"
                     /translation="FPPGQHGLTKKRKKTSEYGIQLREKQKVKYTYGLLERQFRNTFE
                     KAVAASGVTGLVLLQMLESRLDNVVYRLGIAPTRPAARQLVTHRHITINGNVVNIPSM
                     MLRPGDIIGVREKSKSLEIITECLSGNRRSKMTWLEWDDTQMAGKYMSVPERSEIPED
                     IKEQLIVELYSK"
BASE COUNT         5140 a         5209 c         5332 g         5207 t
ORIGIN      
        1 gcccttgaca taagcagggg attccatgcc atcatgcagg aggtgaccga atcaggcttc
       61 tcccggattc cggtctattc ggaaaccctc gaccatatca ggggcatcct gtttgtcaag
      121 gacctgctgc catatctcgg caaacctgac aacttcaggt ggcagtcgct catccgccct
      181 gcttacgtgg ttccggaaac gaaaaaaata agtgaactgc tgaaggagtt ccagacaaag
      241 aagatacaca tggccatagt ggtcgacgaa tacggtggta caagcgggct ggtgacgctt
      301 gaagacgttc tggaagagat agtgggtgag atcaccgatg agacagatga agaggtgccg
      361 ctgtacaagc agacaggtga aggcaaatgg gtcttcgacg gcaagataat gctgaatgat
      421 ttttaccgta ttacaggact ggagaatgac ccgttccagg aagtgagagg cgactctgat
      481 accttggccg gattgctcct ggaactgctc ggggaaatcc cggtcaaggg aagggagata
      541 accgcgggag gcttcaggtt cacaattgaa agcattgaaa accggcgtat aagggagatc
      601 accgctgaaa agaaaaagga agagatatga gaaaaaccat ggcggctgca ctgttggctt
      661 ctgcactgat atttgcagga tgtgaccatc cgacggtgcc aaaaccgaaa ggatacttca
      721 ggattgacct gccgcaaaag agttatgttc attttgactc gccctgctcc tactccatcg
      781 agtaccctga atatgccgtt atcaacctgc acccgatggc cgccactgac acctgctgga
      841 tggacatcga attcccttcg ctgaaggcta agatacacct cacatatttc gacataaacg
      901 ataatctcgc atcgctgacc gagctgtcaa gggaaatggc atacaaacac acccccaagg
      961 ctgaggctat cgatgaaaaa ctctggacag atgaatcggc aggagtttac gggatgctct
     1021 atgacctgaa ggggaatacc gcctcagcgg tgcagttctt tgtcactgac agcatccgcc
     1081 attacctgag gggatcactc tatttcacag cccagcccaa tgaggactca ctcatgccgg
     1141 tgatttcatt catcagagaa gacattattc accttatcga gacgctgaac tggcggtaga
     1201 ccatgggctg catcaccaag cactatctga acagggatac ggttatcggt gtctggaaga
     1261 tcgaagagga catcgatact ctcctttcga tggttgactt cagccccgag gaccggaaga
     1321 gatatgagta cttccggagc aactcaagga agacagaatt tctgagtgtc agggccctgc
     1381 tggcggagat gctgggacgt gaagccagaa tagtctacaa caagaacaat aagccattta
     1441 ttaaagacgg ctcgcatttc atcagcataa cacattccaa caagcttaca gcaatacttt
     1501 taagcaagac cgagagggtc ggcattgacc ttgaatacat gcggatgaac atcaatgctt
     1561 ttgcctcgaa gtttattaat aaaggtgaga agatcacccg caggtgggct gaccgtacct
     1621 atcacctgta tatacactgg tgtgccaagg aagccatata caaaatatgt gacaaggagg
     1681 ggataaacat tgtcaatgac attacaattg aaccttttgt ggtcaaggag tcgggagaga
     1741 tcaggggcag tgtggagaac gagagaatca gtgaccagtt cacgctatat tattcaagat
     1801 acgacaatta cactattgtc tggacgaaga agaagcatga tggttgatat accctggaag
     1861 aaaagaaagc ttattgcccg gctgattgat cctgatcacg ccggtgaaga gacgcttgtc
     1921 actgctgtgc ggctggctga tgaggcaggt gttgatatct ttatggcagg cggcagtctg
     1981 accagtgtgc ctgttactga tgtaatcaga aagctgaaac agctttcatc catcccggtg
     2041 gtgcttttcc cgggaaatct tacacagctc actgccgggg ctgatgcaat tttcctgctg
     2101 tcactcatca gcgggcgcaa cccggaactg ctcatcggca gccatgtgat cgcagcacct
     2161 cttcttaaaa acatgaggga gagagtaatc ccggtgggtt acatgctgat tgactgcgga
     2221 ggtggcactt cagtggagta tatgagccag acatcaccca tcccgtcagg aaagaatgat
     2281 attgctgttg ccacggcact tgcaggagag atgcttggca tgaaggccat atacctggag
     2341 gggggcagcg gcgctgccag acctgtaaat ccgtcaatga taaaggctgt cagtgatgaa
     2401 ctgacaatac ccgttattac cggcggcggg atagactccg ccgaaaaggt ggaggtggct
     2461 tttggcgccg gggctgacat ggttgtcata ggtaatggct gcgaaaagaa cccggagctg
     2521 ataaaaggtg catgccgcgt cagggatagc tataaccgcg acacattcat catctgagat
     2581 tgtaccgcca cggccggtac aacggttatc tgaacagttc gtgcaacgga tctccgctcg
     2641 acgcgttggg caacggcacc ttgcacagta ctgagagggt gatggcaata tccctggtgc
     2701 ttacgctgcg ggtgactgac gccctgctgg ctgtccagcc aaaccagatc agcggcacgt
     2761 gattatcata ctcccagggc gaattatgac ctgtaacatc gtctgtcttc tcaacccatc
     2821 ccggattcag agctatcatc acatcacccg aacgcagctg gttatagttg ttgctcatct
     2881 tgcgcaacag cccgccggag aagtcactcg tctcgaaggc tgaggtggga acggcagctg
     2941 atatgcctga gagctgtacc atgaaacggg ctaccttctt ctgcatatcc tccaggttga
     3001 tcttcgcacc ctcaatgaga ccgcggttaa gatatatctg gctgtcaaag aagcccctaa
     3061 cccagtctcc ctggccatag acggcattga gatagctgcg gagcagctga agtgactggt
     3121 tcagcctgaa ataacctgca ggcaccctgc tctgctccag tactgccgga atctctgaga
     3181 ctccgtgagc tgacaccagg taaataagta cattcttctt gccgagcgac ttgtcaatgt
     3241 tctcaataag cctggcaatg ttcctgtcaa gtctcacaag cgcatctgat gcctcaacgg
     3301 aggaggggcc gaaacggtgt cccacatagt cggtagatga atatgtaatt gcaaggaaat
     3361 cagtgatatc atcctgaccc aactgctcat tgtcaatcag cctgagagcc agttcggtgg
     3421 tataatcatc agcaaacggg gtttggcgga gaacggagaa atctcttgtg acattgagca
     3481 gtttgccttt ggtggagagc tccttcaggt tgtaagggaa ccatgtctgg ccgttgaatc
     3541 ccctctcgag gctgcttgag tcaggctgac atcccgggta tgttgccggg tcaagcaggg
     3601 gcagccattg ctggttgagg agctgttgcg gcatcatcat ggcgttgaag tccatgagcc
     3661 atggcgggag tgaatcggtg taataggtgc ttgacatcca ggtaccggta cggtcatcat
     3721 accagaaagc gccgtctgca gcgtgaccgg ctgtgatgat ggcagacatc tccttcatgc
     3781 caattccgta aactttcgat cctccgcatg aagccatttg cagctcatca gcaaatgttg
     3841 acgagagcag gttgacaggc gagaagaggc cggtctcaaa actgcctccg acagggcttg
     3901 cagatgcatc ctgaacacag aagatcatct catcattaaa cggatgaaac catgaatcag
     3961 aggtgatgcc gtgagctgag ggagaggccc ccgtggatat ggttgcaaat ccgggagctg
     4021 cctgggtgga gaggtagtct attgatgcgt tgcggtaaaa ggtgccttca ttgaccatct
     4081 tcttcagacc gttgtcagga agaatctccc atatgcgttc aagctggtca taacgcatct
     4141 gttccacaat aatgcctatt acaagcttcg gtttgtcagg cggaatgtat gccgactgcg
     4201 ccgtaacgct gacagttaat acaagcagat ataatactgt aattacccgt ttcatcttca
     4261 aagtccctcc atggaaataa gttccaaaaa tatcagtttt gattttatct gcaacgctgt
     4321 gtggaaaaga tatcatcacc ggtcaccggt ttatcaattg tgtgattact gctttgcctc
     4381 tcaccatggg accattgggc tccctcctga cagaaacaat gaagagtccg gagggcaggc
     4441 cggtgaatct gcattcaacc gggatacctg gccgggcggt tgtcctgtag tgtttcataa
     4501 gcgcaccgga agttgaagtg actgtaacgg tcacctcgcc tgtcactgcc acgggaggca
     4561 tgatgttgaa gtttccgtct gacgggttgg gaaacaccag cagacctgat tccggcggtt
     4621 gaggatagtc ctgattgtcc ttctcaagct gaatgtcact gacctgaagg ggactcgagg
     4681 aggtgagctc tgctgtgagc acgtatgttt tatatcccgg gcatgagaag gtgaggttcc
     4741 aggtgccggg aggaaggagg cgtgtataaa acccggtggt gtctgaccag acatgtgatg
     4801 agtcgcggtc atgtccggta atgaaaatct tcgcgctgag cggatcgcct gtttcggcgt
     4861 cagtgaccat gccctttatc cctgtcagcg cttcggcgat atagcgaagc agtgaacggt
     4921 ggttccattc ccacatcagt tccaggtttg agccgggggt catcttggta tcatcaagtt
     4981 ccagtgtcac ttcccgtcct ccaaggcccc atgtgacaaa gtcctgtctt ccgcccctta
     5041 tctggtacca attggatcct ctggtaatgc cgttgtcaag gagggtcata tatccgggtt
     5101 cggaccatag gtgaacggta tctgcgtaac gcctgctgat tgcattgaac caggcatcat
     5161 cagcatggtt tctcgtccat ttgtcccacg gatagttcac gacctcggca ccgctgtgca
     5221 ggttcacaga gagagcgaac cgtctctcct ccatgaatct gatcatgtca agtgtctctt
     5281 tctgcagcgg cggcggcgca gccacctcgg ggtcggggaa gttgcggttg aggtcatagc
     5341 cgttgctgtt ggcgcgtacg gggtagatca gtgtgtcacc gccgcggtac atgccatcag
     5401 ggttggcaag ggggttgatc catatttcaa gttctgacac cagttccgaa gccaaccccg
     5461 ctccggctgc gccgccgctg gaagccaggt attcggcgag acgcatcaaa aggacaaaac
     5521 ctgccgtctc atcaccatgg attgaggagc tgatcatcac ctccggctca ggctcatctt
     5581 cctccgggtt atcagagatc ttcaatacca gcacagccct cccctgaaca ctgaagccta
     5641 tggtatcaag acgacagaag tcaggccact ggccggcaat cttatgcatt atggtgtcat
     5701 agtgtttcca ggtgggatat gactgccagc gcattgcctc ggctaccgat gaggcagtat
     5761 ggaagccctt ccgttcctcc ggcgcaatca gtttgcaggg tatgcctgcc gcgatgaact
     5821 catcaacgtc acggggagag agacagagcg tggctgtcag cccgtcgcat gacgagactg
     5881 agaaacgtgc agccagtctg gtcattgcat cataaccggg atagctgatc atcacttcag
     5941 cctggccgta cctgcttaca acattccgga tatcatcccc cgccgggctc tcctgtccgc
     6001 agaccgcctg catcgaaacc agcagcagga gtaccggtat gatactgtgg agcttcaact
     6061 ttattgttat acgttaaacc ggatgtggag tatgtcacca tcgttcacca cataggtctt
     6121 gccctccacc atcagctttc cggcttcgcg gcatgcatgc tcggagccta gcctgatgaa
     6181 atcatcatat ttcatcacct ccgccctgat gaatcctctc tcgagatcgg agtggatgac
     6241 gccggcagcc tgtggtgcgg tcattccggc agtgatggtc cacgccctta tctcctttgg
     6301 ccccacggta aagaaggtct gaaggttaag catcctgtag gcagccccga caagtttgtt
     6361 cactcccggc tccgtcagtc ccgcagcctc caggaactcc agacggtctg tctcttcggt
     6421 gaggttggct atctccgcct caagcgcccc ggcaatcatc agcggctcag cgcccctcgg
     6481 ggcaaggaag ctttttacat catcaacata cctgttcccg tcaacagccg aagcctcatc
     6541 aacgttgcac acatagagca ccggcttcat ggtcagcagg aagatatcat ccacaacctt
     6601 tttttcctca ggtttcagtt caagggagga tgcgttctga aaaccttcga gatgctccct
     6661 gtaaaggtta agaatctcaa tgccgtgttt tgcttcttta tcacctgttt ttgccgcccg
     6721 ctccagccgc tgtatctttt tcattactga ctccacatcc ctgacctgca actcaaactc
     6781 aacgttttcg aggtcacgga ccgggttgac agatccgtct atatgcggga gattgtcatc
     6841 atcaaagcac cgtagaacat gaatcagggc atcggtattc cgtatctctc ccagcaggcg
     6901 tgcccctgaa ctgtcaccct gccccctgac cagaccaggg atgtcaacga tgtcaaccgt
     6961 tgtgtgcact atccgttcgg tcggctgaaa cttttcgagt tcgtagagcc ttttgtcagg
     7021 cacctttatt acgccaaggt ttgcctttac tccggtaccg tatgtgtctg tctcaccgcg
     7081 ggtgtttgat atacaattga agatagtggt cttccccaca ctggtgatcc ctattattcc
     7141 gcatttcaat gccatatctc ttgatgtaat gctgcaaagt taccaaaaag aggctaagtt
     7201 tgaattgcag attaaaccat tggcgcctcc gggcgtctga atgattatac cgccacggaa
     7261 ggttaatgcc gcaaattact gaagagaata tcaggattac tgaagataac atcagggaag
     7321 ctctggcaac ggatgccgat aaggcattcc ggatgattat tgatctatac gggcggaggc
     7381 tctactggca catccgccgg cttgtaatca cccatgatga cgctgatgac gcgctgcaga
     7441 acactttcct gaatgcatgg aaaaacatcg ggtcattcag gtttgacagc tcgctgctca
     7501 catggctctg gacaattgcc accaatgagg ctcttactct aataaggcga agggcacgtg
     7561 agaagagcgt cgcatttgat gatgtcagcg aaatccttgc tgcttcggct gaaggaagca
     7621 cttggtttga cggcgatgcc gcgatggtca gacttcagaa tgctatcctg aaactgccgg
     7681 aaaagcagcg tgctgtattc aacatgaggt actttgatga gatgagctat gaggatatga
     7741 gctcagtaac aggaacctcg gtgggggccc ttaaggcatc ataccaccac gcagtaaaaa
     7801 aaattgagca atttcttgaa cgcgattaaa cttattcccg gtcagggtat caaagatatg
     7861 gtatgaaaca cacatgtatc aattacacaa acagctgcct gtataatttt cagttataca
     7921 ggttctgaca tacccttggg ataaaattat aatatgatga aaaccacatg tatttcatac
     7981 acaaacacgt atgtatataa ttttcaaaca tgtgggttcc atcagaccat tgaaataaaa
     8041 attatatact gatgaaactc aacgatgaaa tattaaaaaa taatcccttc agggtgccgg
     8101 atgattactt cgacacgctg gctgaccgta ccatggccgc gatagacaag gaggccggaa
     8161 aagatgggaa gagggccgta ataaacatga aggccggaaa agatgggaag agggccatga
     8221 taaacatgga ggccgcaaga gatggggaga gggctgcgat aagtgaggaa aatatccgta
     8281 agccgggcag ggtgataagg ctgaaaccat tcatggccct ggcagctgcc atactgggtt
     8341 tcgcaattct tgcaactgca ctggtcaggc tgataaatgc tgaccggccg ggcatggtga
     8401 ctgattccgg ccacagcctc tatgctgacc tcatcgttga agagatagac acatacatgc
     8461 ttgaagatga gctgcacatg actgacccgg ccatcccgga gatgactatg gagacaattg
     8521 agtcagaggc tatcattgat tacctgatga ctgaagacat cactcttgat gacatatatg
     8581 aactgttata ataatgctat gaagagaatc attacaacca ttgccgcact gttgctgacg
     8641 ggagtcgcag ccatgaccct cagggcacag ggccagggta tgggccaggg tgccatgcag
     8701 ggtgacagtg agaaactcac cgcctacaag attgccttct tcacaaagaa cctggacctt
     8761 actcctgccg aggcggaaaa attctggccg gtctacaatg attattcggc gaagaagagc
     8821 aagctgcaga ccgaccgtat gttgcttttg cgaaatgcgg ctcatcacca ggccagcatg
     8881 actgaaaacg aggttgcatc ctctgccgat aagctgataa agtcatttgc cgatgaggca
     8941 gccctggtgg catcattcta tgagagcctg aaaaaggtgc ttccaccagc caaggtgatc
     9001 aggttgtgtc aggttgagaa ccagtacaag cagcatttgc tgcgagagct gaaccagaga
     9061 agacagggtc agtcacagaa gggacagggt ttgaggagga gcagcggtca ggcaggatct
     9121 gccgcgttga ccgagtgatt gttcaggagg tcttcacccg gcgtatcagg aggccaatac
     9181 ccggtgtgtc aaaagatcac gacctggtgt atctagaggt ctttacctgg tgtgtaacaa
     9241 ggtctttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag atcacgacct
     9361 ggtgtatcta gaggtcttta cctggtgtgt aacaaggtct ttagccggcg aatgaatgag
     9421 gtgagttcat ggaacgggac agctgtctgc tctcccgttt tcatgttttt gacggtagcc
     9481 tgcccggcgg ctaactcatt gctgccggct atcaccacaa gaggaatcat gcatgaatca
     9541 gcgtatgcaa actgcttcct gagctttgcc gggtcagggt agagttctgt ggcgatgcca
     9601 gcctccctga gttcaccggc aactttcatc agtgcaggca gttccgcgct gccaaaattg
     9661 atcacgagca cctctgtgga tgagccggtc tgctccggga gcagattgag ctgcatcatc
     9721 acgtcataaa tcctgtcggc cccgaatgag acaccgacac ctgacacgcc aggcatgccg
     9781 aagatgcccg tgaggtcatc ataacggcct ccgccgcaga tgcttgcgaa gggaaggtct
     9841 gctgcactga cctcaattat ggttccggta taatagttaa gtccgcgtgc aagcgagaga
     9901 tcaagctcca cagttgcctt taccggttca actgcaagat atcccgtaag ctcctccatc
     9961 tccctgatcc cctgcatacc tgaagctgat tctgccatag ccttcttcag cacggcaagt
    10021 ttctgttcgg ttgagccgct caggtcaatt atcggttcga gcctggcaat caccccgggt
    10081 gcaatgcctt tcgcggccag ctcctgcctt actccttccc tgcctatctt ttcaattttg
    10141 tcgatagcca cggtgatatc ggtgagcctc tcgccagctc cggcaatttc agcaatacct
    10201 gccagtatct tgcggttgtt tatcttaata atgatgttaa tgccgagctc gccaaaaatg
    10261 cggtctgtta tccgtaccaa ctcatcctca ttaagcagtg agtcgctgcc gatcacatcc
    10321 acatcgcact ggaagaactc cctgtacctc cccctctgtg gcttgtcggc cctccagacg
    10381 ggctgtatct ggtacctgcg gaacggaaat gtgatctcgt cacgatgctg caccacataa
    10441 cgtgcgaatg gaacggtgag gtcatacctc agtccctttt cgctgatatg gcgggtcacg
    10501 gcagatgtat ctgcagccga gagtgccgat ggtgacacgt cagcaagaaa gttacccgaa
    10561 ttgaggatcc tgaagatgag cctgtcaccc tcttccccgt atttgcccag aagggttgag
    10621 aggttttcca ttgcaggggt ctctattggc agatagccgt agagcgaaaa tatcttcctt
    10681 attgtatcga agatatagtt ccggcatagc atctctgccg gagagaagtc gcgtgtgccc
    10741 ttggggatgg atggtttctg agccatgacg ggaaatcgtt acccggcaaa aataggaaaa
    10801 aaaggtggat ggggcatatt acccctgccc atatacaaat catgaatcat catcacgtaa
    10861 cggcaggtct cagacctgcc gccacctgcc ccatatgcaa atcatgaata accatcatgt
    10921 agcgacaggt ctgagacctg tcgctacatg ccgccaccga atcataatga taatttgttt
    10981 attggataaa ggggattgat atcgggtatt tgaatacctc gccctgtgca gccttaatgc
    11041 ttgccacaat gacgcatacc atctcaagga tgccaagggc aaccaacagg ggaattccaa
    11101 caatgataaa acaaagcgga atgagtaaaa tggtatacag taagattgaa atctggaaat
    11161 taagtgctga cttcccgttg gtattgaccc attcggactg atccttcctt gatatccagc
    11221 agatcagcgg cccgataatc cccccgaagg ggaagaaata accggcaaag gatgacaggt
    11281 ggcagaacat tgcccagtcg cgctctgact gtgtataggt tttgatttcg tccattgctt
    11341 tttttcttaa agacggattc cggttaaaaa tgctgcatca tcagaagcgg gcaagcctct
    11401 taagatcgga aatcagcagg ccgctggcat ttcttctgtc aaccagatga tcgtcatagt
    11461 aatacattat tgcatcatct gtcccgatga gggttttcat acaggtacct gtctgcttga
    11521 cgccgtcggg gcccaccttg tgaacaatca ggacatcctt ctgcttttct tcgatggctt
    11581 gaatgatttc ctcttcatcc acaatcctga ccttgtaggg ataatatgac gcaatttttt
    11641 ccggggtgtc aatctcgggg gccaggtcag actgccgtac caggatggtc ttgtccttta
    11701 tttcaggtac attcttgtta taatatttga gataccgcaa tgctgagagt gttgggttct
    11761 tcatcaccag atcggcatga tattccatga accggatgat aaggccgagc ttgtaactgt
    11821 attcctcttc aggggcaccc gagaagcaaa gtggaatggc gcagaattcc ggcagctggt
    11881 caagttcgtc aacgtcagcg cccagcaaca ggttcaggaa gtcataggtg gagcctgact
    11941 tgtcattgct gaagttggaa atggtaagaa ccaggaaaga gtatgccggg tcattgcgca
    12001 tgacgttgaa ctcctccgtg gtaatgtatt tcaccggggt tgccttccag tatttgtcta
    12061 cggcagcctt tatttctgca ttgtaaaatg agaagggatc atcttccctg acaatgcatg
    12121 ttactgattt tttgaatttt tcagcgtcat caaaggtagg gtatggcctc ttttgtgcga
    12181 agatggctga agccagcagg agagacaggg cgagggtaga gatttttttc attaaacagt
    12241 tattaattcc ccgaaggaag gaaaacaggg tttctttcat tgaacttcct caggtagaca
    12301 aacatcattt tcttctggtt tttgttgcgc attgacacaa actcttcgta cagctccggg
    12361 tcccgcataa ggagcgcttc cagattatcg aggttatact cgtacgtctt ccccgtctca
    12421 aaatcaatca tgaacatttc aaggtcagta cgtgacgtgt gccggccata gggagatctg
    12481 ccgtagtagt acgggtcata atagcgcgaa tagtatgggt tgtaataata tggcgagtag
    12541 aatgacctgt aaggtgagta aaaataggga tcatagtaac cgtaggggtt gtaacctgtt
    12601 tgggtggtga catctgccac gaaatggatt attgacccga agaaggtgat gcggttgaag
    12661 ttgttctgca cctttgcata gagaacaccg ttccgggcga agccccagat cgcatccttc
    12721 tctatctcct gcctgacacc catgttatca tagaaatata tcttctcgga ggagatcacc
    12781 tggtcaaaaa aatccttgtc gttgtaatca accgaagtga agagtttagc ctttgggatg
    12841 gggctgtttg tctttaccat gtcaaaattc aggtatatac cgtccctgaa cttgaagtca
    12901 ggagtatact gaacaagagt atccccctgc tgtcccttgg ctaaaccggt ggcaaccaag
    12961 gctataatca gtgtcagtaa gcactttttc atcacactct tttacagatt ttacctcaaa
    13021 aactataccg taaggcaaaa ataatctttt ctccgtactg atgccagagc tctctttatc
    13081 tttaatgaaa ttgatgtata tttgagtgac tttaatctac tgtgttcacg atatgccgaa
    13141 cagatcaaga tcatccacca taccgcactc agacctcatg aaccggtgga cgcgtgaccg
    13201 tatactgctg ccggttgcgg tagcgcttct cctggtggtc atagcccttg tcaccgccct
    13261 tgccgggctg gtatacgtgc cgatagcggc aggtgtgctt gccctggccc ctcttgtgct
    13321 ggcgatagca aaattgcgca agctcattgc agacaatatg gcgattttcc gggagctggc
    13381 tttaattgcc gaaaagaacg aggaggtgat aaactccttt tcgcacaaga taagggagcc
    13441 gctcaacaac ctcgtcctgc ttggcagcat gctgagtgag accaccacat cacccaagca
    13501 gcaggagctt gtggatacct tcattgcttc caccggatca atggtggagg tagtcaatga
    13561 acttaccatg gaaaccgccg gcaatatcac ttttgagaag cggggcgaga tttgtttcag
    13621 actggggccc atcatccgtg ataccattgg gctgatgaag ctcagcagga gacatgaaca
    13681 gagcgtggtg gtctatgatg accgcggcga gggagaaaca gaatacaggg gtgacccgat
    13741 catcatcaag cagatactca tcgatcttct aagcactgcc atctcaggac cgtcagaaaa
    13801 ggtgactgtc aagatacagg tcagggatgc cggtgaaagg gatggagtac acaagcttga
    13861 attcaccctg tcatcgaaca accagatagt cttcatcggc aaggatcgcc agccttaccc
    13921 cctggcagga agactgatac gcgattgcgg gggcaaatgg caggaggcaa cttccggaac
    13981 cggttcatat ttcgtcttct atatcttcct gcggagggca gaaaaggtgg cccgcccggc
    14041 tgcagcaagc tcccacataa gagagatgga gacagagaag agccgtaagg aactgaaaga
    14101 gcttaacata cttctggtgg aagacaaccc catcaatcag cgtatcaccc agctgatgct
    14161 tgaaccgctt gtgaagaggc tcgaggttgc ccgtaacggc aaggaggcgc ttgatatgtt
    14221 tggcacagca cgctatgacc ttatacttat ggatgtgcag atgccggtga tgaacggact
    14281 ggtggcagcg gagaagatac gagggctgga gatgagctcc agcggacacg tacccatcat
    14341 tgccattacc gccaatgcaa tgcttggcga cagggaacag tgcctggctg tcggtatgga
    14401 cgattacata agcaaaccta tagaaccaga ggtgctcata gagaaaataa cagctcttat
    14461 ctgacctcag aaaggcttga cccccaggat cataatgtca tcaatctggt catgcccttc
    14521 catccatgac actatagttt catccagtat cctctgctgg tcagtcagtg gatatttact
    14581 tattgtcagc aggatatgcc tcagacggcg atacataaac ttcttgtttt ccggccctcc
    14641 gaactggtca gcatacccgt cggagaagag atagaggata tctccctcct gcagggtgaa
    14701 agtactacga ctgaatgata ccatggcaaa tgactgaacc acattcttct tgtctccctt
    14761 gatctctatc agcttgtcat cacggactat atacaccggc agaaatgcgc ccgagaactc
    14821 catcactttc gtgtttttgt caataatgca gatactcatc tcaattccat cctttatcag
    14881 ggctttgccg ctttcctcct tgaagaaggc tgattcaagc tcacggttca tggtcatcag
    14941 cacctggtct gtatcgtcaa ccttcaactc attgacaatc ttctggatga gctccagtcc
    15001 gatcattgac ataagcgctc ccggcacccc gtgcccggtg cagtcagctg ccgcgatgaa
    15061 gtacttttcg tcacgctcac tgatccagta gaagtcgccg ctgacgatgt gcttcgggcg
    15121 gtaatagatg aaatgatcgg gaaaaattct tcgaatgtga tgttcggcag ggagaagagc
    15181 agcctgtatc ctgcgggcat agttcatgct gtcagtcagc tccttgtttc gcagttgcag
    15241 ttccgatctc tgagcctcta tctcgccgta tgcgccctgt atctcacgaa ggtgcttctt
    15301 ggtacggagc aacacccatg actggagaat gatcagataa accagtgctg caatgataag
    15361 gaacacaatg aggctgttga gagccgtgct gtctctcacc ccctgcacag tggcagccaa
    15421 caggtccgtt gaagataagc ctgtcacccg gcagttgaat taaccgtctt tacggaagct
    15481 tcgccgggca tcagcagggg aaggagcctg agagacggtg attcaatgaa aggaccgagt
    15541 ccgtattcag gccacctgga gttgacaagt tcaattgttt ccgggtctga acagaccacg
    15601 tttggccaat ctctggggaa aacaggcagg caacgggttt ttgaagtggc atcaatgaag
    15661 atggcgccgc cctcaagatg atagagatca cgggccggat cactgttgcc ggtaaccagc
    15721 cacgtgagca tggcggggtc atcagcgtca gctccatcat ccacagcaat gatgacggtg
    15781 cccggtgtgg taagtgatgc gggcaaagag gcagccagga gatgcatgtc aagcccctcc
    15841 tccggtttgg ttattgtcag tattgccacc ggcagggcat aatcagccag cgtcttcatc
    15901 ccggtaatga ccttttcatc gaactgccag ccggtgagaa ggagcagcgg tattgaatga
    15961 tactcatcga tccgttcctc cggaagcttc agggtagcgt caatgccaag cttgcctccc
    16021 atgctgaccc tgtctgaaga gtgatcaagt acgtcaagag ggccccttgt aagcagaagg
    16081 tcagaatcaa acctcacatt ggcaaaaacc tcctcggcca cggcgaggta atcagttaca
    16141 tgaacgttgc cgctgactac tattatatat ttgctgagca tcatctgccc tgcgccgaaa
    16201 agcgcactca gcactttcat cccctggccc ggatagctct tctcaatacg cactatcacc
    16261 aggttgtggg caacgcccga ggcaggcatg tggagctcgc gaatctccgg ggctattgca
    16321 attttgatgg gtgcaaagaa tattttttca gtagcttttc ctatccaagc atcctccatg
    16381 ggaggcactc ctactattgt ggcgggatag actgcatttt ttctgtgggt gatgcaggtg
    16441 acatggaaca taggatagag gtcggcaaga gaataaaagc cggtgtgatc accaaacggc
    16501 ccctcggtga caggggcttc ggccgtgtca acatatcctt ctatgacgaa atcagagtcg
    16561 gccggtaccc acaggtcgtt ggtaaggcat ttgaccaggg tcactctctt acggcgcagg
    16621 aatgctgcaa ggaggtactc attgatatct tccggcagcg gagccgtggc aacataggta
    16681 taaaccgggt ctcctccgag ggttacagtg acaggcatcc tccctcccct cctcttccat
    16741 gcctcaaagt gcctggcacc ggtcttgtgc ctgtgccagt gcattcctgt cctgtcacgg
    16801 cccatgacct gcatgcggta catgccgagg ttgtattttc cggtctcagg gtttctggta
    16861 tgcaccagtg ggagggttat gaacctttcc cggtcatggg gccagcattt caggatgggc
    16921 agcctgtcaa ggtcaggtgt ctgctccacc acctcctggc attcacccct gcctgacttt
    16981 ctttttggcg acagggtgag aagacggagc atccccggca gacgcgccag cttgcccgtc
    17041 agaccctgcg cacgggtaag ggattcaacc agggcaaaca gctcatcgcc tgcatcatca
    17101 agggaattac gtccaatggc agctgccatg cgcgtgtcag aggcaaatgt gtttataagc
    17161 acagggaagt cagtgccggt attctcaaac agaacagctt tgcctccatt ttttacgaga
    17221 cggtcagcta tctcggtaat ctcaagcaca ggggcaacag gggaagtaac acgcaccagc
    17281 tcaccacgtg attccagaaa tgacacaaac tgagacagac cgttgaatga cattgaataa
    17341 gagatttatg caaatataaa aataaaaact ccgccccggg ccgtatcagg cccgcaccgg
    17401 cggagttttc atattatggt aacgtagcgg gagcggttac ctgtaaccgt agactgcatt
    17461 gtcaccaagt tcttcctcaa tgcggagcaa ctgattgtac ttggcaacac ggtcagtacg
    17521 gcagagtgaa ccggtcttga tctgacccga atttattgca actgaaacgt cagcgatggt
    17581 atagtcttca gtctcgcccg aacggtgcga aatgacggtg gtgtagcctg ccctgtgagc
    17641 catctcgatg cagctgagag tctcactcag ggttcctatc tgattcagct tgatgagaat
    17701 actgtttgcg cagccaaggc ggataccctt ctcaagatac ttaacgttgg tcacgaagag
    17761 atcatcacct acgatctgga tctttgaacc gagctgatcc gtatgcattt tccatccttc
    17821 ccagtcaccc tcactcattc cgtcctcgat ggaatctatg ataggatact tctcaaccag
    17881 cgcagtgaga taatcagcct gctgtgcagg ggtgagcttc ttggcatcct tgccttcgaa
    17941 cttggtgtaa tcataaatac cattctcgaa gaatgaactg gcagcacagt cgagcgctac
    18001 catcacatca acagccggct tgtatcctgc agcagtgatc gccttaataa tacaatcaag
    18061 tgcatcctcg gttcccttga gtgcaggagc aaaaccaccc tcatcaccta ccgcagtgct
    18121 gagaccacgc tccttgagca ctttcttcaa tgaatgaaac acttcggctc ccatcctgag
    18181 accctcagca aaagtgccgg cgcctttggg acggatcatg aattcctgga atgctatcgg
    18241 tgcatcagaa tgagcaccac cattgatgat gttcatcatc ggaatgggca gcacatgggc
    18301 gttggttccg ccgatatacc tgtagaaagg caatccgtga taggctgctg ctgccttcgc
    18361 gcatgccagt gacacgccga gggtggcatt ggcgccaagg gtactcttgg tgggtgtacc
    18421 gtcaaggtca atcatcttct ggtcaatgac tatctggttg gtaacatcca ttcccatcaa
    18481 ttcctcagct ataacattat tgacattatg gacagctttg aggacaccct tgccaagata
    18541 acgcgactta tcgccgtctc tcagctccag tgcctcgttc tcaccggttg atgctccgga
    18601 gggtactgcc gccctgcctg atgctccgct ggttgttatg acctcgacct cgatggtcgg
    18661 atttcccctt gaatcaagga tttctctcgc atgaatacta actatctgac tcattgtaac
    18721 ttatgtttaa ttatgattat acgcaatata caaaaaaaag ggatacggtc attaagacaa
    18781 tatcccttcc atatatgagc atctgctgca tataagcatc tgctgctatt cttttgaatc
    18841 ttcggtttcc gctcctgttt cttcggccgg agtttcagcc tctgtggcag caacttctgc
    18901 ggtagtttca gttgcggcag cagcttcggc cggagcttca gccacgatag tttcggtagc
    18961 agcttcagtt acggcagttt cggctgcggg agcaacttct gcgctctttt tcctggagcc
    19021 gcgacggcgg cgtgaagccc tggttttcgt gccctctccg gcagcaagca tgttctcatt
    19081 gaagtcgacc agttcaatta tacacatctc agcagcatca cctatgcgcg atcctgtctt
    19141 aaggatgcgg gtgtatccgc ccggacggct ggcgatcttc ggagctactt ccctgaagag
    19201 ttcagcaacg gcatttttat tcccaagata actgaataca accctgcgcg agtgagttga
    19261 gtcatccttt gacctggtga tgagtggctc aacgtatgtt ctcagtgcac gtgctttggc
    19321 tgttgttgtg ctgatacgct tgtgaagaat cagtgatgat gccagattgg ccatcatagc
    19381 cttcctgtca gcacttttcc ttccaaggga gtttatagca tttaaatgtc gcatttctct
    19441 ctatccttac ctgttattaa tctttgtcaa gtttgtattt tgtcacatcc atcccgaatg
    19501 agaggcccat tgtcgcaagc agatcttcaa gctcggtaag tgacttcttg ccgaagttgc
    19561 ggaacttgag gagatcattc ttgttgaatt taaccaggtc tccgagtgct tcaacctccg
    19621 ctgccttgag gcagttgaga gctctgacgg agaggtcaag gtcaacaagt ttggtcttga
    19681 gcagctggcg catgtggagt atttcctcgt caaattcctc atttgccatc ctgtcttcgt
    19741 agtcgatggt gatcttctct tcggagaaga gcatgaaatg gtatatcagg attttcgctg
    19801 cttccttgag tgcatccttc ggatggattg agccgtcagt atttatttcg atgattagtt
    19861 tttcgtagtc ggttttctgt tcaaccctgt agttttctat tgcatacttg acgttacgga
    19921 taggggtgaa gattgaatcg atggcaataa gtccaaattc tgcttccgcg ggctggtttt
    19981 cctcagcagg cacatagcct ctgcccttgg agatattgat ctcgatctgg agcttgacat
    20041 cgggttccat gcggcagatt accagatcag ggttcagcac ctcgaagctg ctgagtgaat
    20101 tattgaggtc accggctttg aatgcttcct gccctgagac cttgactaca atcttctcgt
    20161 tatcgatatc ctcaaccatt ctcctgaaac ggatctgttt catgttaaga atgatttcgg
    20221 tgacatcttc catcacgccg gggattgttg agaactcatg gtctacaccc tctatcttga
    20281 cggtagtgat ggcgaatcct tccagtgagg agaggagtat tcttctgagg gcattaccca
    20341 cggtgatgcc atagccgggt tcaagcgggc ggaattcaaa ctttccgtac tgatcgtccg
    20401 cttccagtaa tattactttg tcaggtttct ggaatgctaa tatggccatt atcgaatatt
    20461 attactttat tacttggaat agagttcaac tataagctgt tccttgatat cttccggtat
    20521 ctcggaacgt tcagggacgc tcatgtattt tccggccatc tgcgtgtcat cccactccag
    20581 ccaggtcatt ttggaacggc ggtttccgct gaggcattct gtaatgattt cgagtgattt
    20641 tgacttttcc cggacgccga tgatatcgcc ggggcgaagc atcattgatg gtatgttaac
    20701 cacgttgccg ttgattgtga tatgacgatg tgtcaccaac tggcgtgctg ccggtcttgt
    20761 gggagctatg ccaaggcggt ataccacgtt gtcaaggcgt gattccagca tctggaggag
    20821 aaccaggccg gtaacaccgg aggcagccac tgccttctcg aaggtattgc ggaactggcg
    20881 ctcaagcagg ccataggtat acttcacctt ctgcttttca cgcaattgta tgccatattc
    20941 cgatgtcttt tttctctttt tggtcagacc gtgctggccg ggaggaaa
//
LOCUS       DAOPBK010000020        20647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2706_length_20647_cov_5.316288, whole genome shotgun sequence.
ACCESSION   DAOPBK010000020 DAOPBK010000000
VERSION     DAOPBK010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20647
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_20647_cov_5.316288"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLA17_02185"
     CDS             <1..573
                     /locus_tag="PLA17_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPK84252.1"
                     /translation="IYTVRESLWMSVPSIAGALLGAVIAVKINERVMELLIAGLLVFM
                     FFVILYKPEKWLRDQAQSAIMQRRWWVPVLFFIIGIYGGFIQAGVGFFLLAALVLGTG
                     MGLTRANAHKSLIVAVMTAISLVVFVFSNQIYYLYGIILAVGQGAGAWLGSKVAVSWG
                     PKVVRVILLVCIMVSAIELTGLYDLVAGWF"
     gene            complement(586..1488)
                     /locus_tag="PLA17_02190"
     CDS             complement(586..1488)
                     /locus_tag="PLA17_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPK84253.1"
                     /translation="MPLLRNNTSEPLTKERKQGLIFSALTHLLLAILIIFVTFRVRIP
                     DPVEEGLLVNFGFDETGEGLFEPAPEPVSPPPPPESAWEQGGEEALLTQDFEEAVEVE
                     KKEPGPEEIKRLAEAREAEIKRQLEAEARAEAERKRLEQEEAERRKREEEQRRIDQQN
                     ARARGAFSNAGNVGTSGQSQGVAGGRGNQGVESGTPDAPNYGPGGGQGTDGISFDLGG
                     RKAQSLIRPPYDLQKEGVVVVAITVDRSGRVTDATPGIKGSTTLEVDLLKLAKEAALK
                     TRFESSNNAPIIQKGTITYVFRLK"
     gene            complement(1469..1861)
                     /locus_tag="PLA17_02195"
     CDS             complement(1469..1861)
                     /locus_tag="PLA17_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPK84254.1"
                     /translation="MAITNKNKVSIQFSNAGMSDIVFLLLLFFMLTSTLVHPTALKLL
                     LPKGTTQSSARPMTTVSITKDLQYYIEDQPIDLEFLESALRSRVQDSPEIYISVHADR
                     SVPIEYVVNVMNIAAKNQFKLILATTPK"
     gene            complement(1865..2557)
                     /locus_tag="PLA17_02200"
     CDS             complement(1865..2557)
                     /locus_tag="PLA17_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPK84255.1"
                     /translation="MISAAILLQGTPVTEATEVTKEATMSLADLYLAGGWVMYLITLL
                     SFVALYIFIERYLVIKKASKSDANFMNRIKDYMYEGKVDAALQLCRRTDSPQARMVEK
                     GITRLGRPLADVTVAIENVGRLEVHKLEKRFPTLATIAGAAPLMGFFGTVIGMVQAFY
                     EMSLAGSSLDISTLSGGIYTALITTVGGLILGILAYFGYNQLVVKVENVVFQLETTST
                     EFLDILNEPVKE"
     gene            complement(2684..7204)
                     /locus_tag="PLA17_02205"
     CDS             complement(2684..7204)
                     /locus_tag="PLA17_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPK84256.1"
                     /translation="MAKIIKKIIRGLLIITAVLVVVPLILFLLLQTPLIQTWTVNRVT
                     KAVSRETGAEIMIGRVSYSLWHEIVLDDVLLKDLNGDTIIAARKVDMRLREIRPSQKI
                     YKFRTIDLHEPDFRMIRDTAGVMNLTAYLQSLKSDRERDSTRNIEIHFADIDLLNGSF
                     TLSDMSDTVGVIPGGVNYRNMYIRHITGKVNDLRVIPDSVSMVIRGLAFQEDGGFISR
                     SLDMNMAVAESDLYFRDFDLLTDSSAISAEKILIMPREKDSWKDFINDVRLDFLFDNS
                     IVNTSDISYFVRPLNGVSEKVSFSGRVTGTVAEMKGKNIRIDYESSTRLRFDFDISGL
                     PEINDSYLYISFTDMKTRAADIERFTLPGRKPFRLPAVAHDIGLVTYKGSFTGFTTDF
                     VSFGRFTTERGTFDTDLSLKPDGRNAFGFRGSLRTSAVDLGYMTKKSEMFGGLWMHAE
                     VDGTMKSFRHLSANINGVIDSVEINKYLYRNITVSGTYANKIWDGSVEVNEPNIRMSL
                     LGRFDLEKSKPEFDFTMNLASADLHTLNLVTKDSIFRATALLTANFRGNSMENLEGDL
                     RLINSTLENSNGKLNIYDFLVSSEIVGGVPFLKLRSDFADADVRGKYSVDAIKRSVAE
                     IKADLFPSRFSKPVARKGESTSEAMFSLDTRIKRIDRLNEFFGTGLSISEGTLITGHF
                     WSDRPEVIAGIEADAVTYLGVRMGRLNMTGSLQNGSMKLDLAADTVTLPDNSELGNFA
                     LGAISSNDTIDLGMNWDNHDNGRTLGEIRVRGFFGHSAGNLPSLTVAVLPASLTVNSS
                     QWNITPARIVIDSVSAYFDNILVSSRTKYFSLGGRLSKDPDEKLTLTFEGLNMSYLNN
                     LLQGKQDTKKPAQMKFEGTMSGNLTLSDVYNNLLFESSINITDFRLNDNNYGLMTIIS
                     EWDPGRKVAVINMGNDFAGKKLIDVSGTYSPTARVIDISASASDLPLDVLNPFLSSFA
                     SDIRGFGTGTIRLNGKLRQLEMTGAVMAHDASMKIDFLQTRYSFSDSVRFSPAGIILR
                     NINIYDTQKNQGMINGMITHRAFRNFGVSLDINMDKMLVLNTRPKDNDSFYGTAYASG
                     YAGIRGNDEKLVFNISARTAPDTEFFVPLNSSAKVSDYPFIEFLKPAGETGKQEQADR
                     HGSFVRQEEGSKIELNFDLDVSPEAEVQLILDETSGGVIRGKGEGKLNISLNSRGDIR
                     MAGDYVISEGDYLFTLGNILNKRFAVEKGGTVSWNGAIEDASLDIRALYKTKASLSEI
                     FGEEEFTELKAKMPVECVLSLSGRLLNPGISFDISLPTADERTRELLRLAIDTEEELR
                     RQFLYLLVMNSFCPDPSLYNSGAATGTPLAQGGYAASPIAGGVTTTTEMLSNQVSNWL
                     SQISNDFDIGFSYRPGSELTDQEVELALSTQLLNDKVTVNGNVDVRGNQSNTYTKNIT
                     GEFTVEVRLTDMLRFKVFNRSNHNLYYQVHPYTQGVGLFFRRDFSTLKDLFIRPDEKK
                     KKVNGDGESGKD"
     gene            7257..8282
                     /gene="tsaD"
                     /locus_tag="PLA17_02210"
     CDS             7257..8282
                     /gene="tsaD"
                     /locus_tag="PLA17_02210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPK84257.1"
                     /translation="MTPQITILAIESSCDDTSAAVICDGFVLSNLIANQDVHKAYGGV
                     VPELASRAHQVNIVPVADQALARAGKRADEVSAVAFTQGPGLLGSLLVGTSFAKGFAL
                     ARNIPLIAVNHLQAHVMSHFAMEHGRENRVPQFPFLCLLVSGGHTQLIVLRDHLEMEI
                     IGNTIDDAAGEAFDKCAKVMGLPYPGGPEIDRLATDGNPDAFRFAKPRIQGLNFSFSG
                     LKTSFLYFLRDRVAEDPGFIGKNLNDLAASLQRTIIEIIIGKLVRASRETGIREIGIA
                     GGVSANSGLRRAATREAEKRGWNLFLPELRYTTDNAAMIGITGYYKYLKAEFTDHSVI
                     PAARIDT"
     gene            complement(8239..8751)
                     /locus_tag="PLA17_02215"
     CDS             complement(8239..8751)
                     /locus_tag="PLA17_02215"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPK84258.1"
                     /translation="MERRRKIINPFDADQNTCFGCGPKNQIGLKLLFEEDDEKVYASW
                     MPDRQYQGYINVLHGGIIATLLDEVSAWCVYLKACTAGVTSSMTVRYLKPVHISKGPV
                     TVEAMITKREEKSALISAALHDGEGKLCAEAELDYFIYPEKVARARYHYPGREAFKYQ
                     SEPPESPNGQ"
     gene            complement(8726..9313)
                     /locus_tag="PLA17_02220"
     CDS             complement(8726..9313)
                     /locus_tag="PLA17_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPK84259.1"
                     /translation="MNLQDYYRILGIKQNATDDEIKKAYRRKAMEYHPDRNSSPDSQE
                     MFIRVTEAYEYLMSHPYRRNITEEEIMRNYQAWIDYRREEARRRAEAYAKASYAEFRK
                     SPVYRSTTAIDGTIVFLGLILATMVIVTTVYGYIYRMRIADTPKEEPSMTLAAISLII
                     GFSYLIISIFYLSAWIARQKKKRRKTEDGAQKKNN"
     gene            9428..10294
                     /gene="zupT"
                     /locus_tag="PLA17_02225"
     CDS             9428..10294
                     /gene="zupT"
                     /locus_tag="PLA17_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPK84260.1"
                     /translation="MDSSRLLFAFGITLFAGLATGIGGVIAFTARRTNTTFLAFSLGF
                     SAGVMIFISFTEILTEAGDIMRLNYEANTAAWLTFISFVAGIGLSAAIDKILPSEGNP
                     HELKRVEQMHPESLNPDKPECERRKNRNRGGHCIGDRKLMRIGTFTAIAIGIHNFPEG
                     IATFMSAMSDVAVGISIAVAVAIHNIPEGIAVSVPVYYATGSKRKALAWSVLSGFSEP
                     IGALVGYFILRLFNTSISLGYVFAMVAGIMVYISFDELLPAAHKYGKHHVAIYGLVSG
                     MIIIGISLILLG"
     gene            complement(10327..10479)
                     /locus_tag="PLA17_02230"
     CDS             complement(10327..10479)
                     /locus_tag="PLA17_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84261.1"
                     /translation="MVTLAGTGTERLRAGVVTLAGTAAGQLTRAWRSYGPVWLPWQAQ
                     ARLPDS"
     gene            complement(10531..11784)
                     /locus_tag="PLA17_02235"
     CDS             complement(10531..11784)
                     /locus_tag="PLA17_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HPK84262.1"
                     /translation="MGLKNRLIIMNFLQFFIWGSWLLTIGAYWFQNKNWSGTDFGAIF
                     STMGLASLFMPAIAGIIADRWINAEKLYGIFHIAGAILLSLIPAVNDPDTFFWLLLLT
                     MMFYMPTLALAITVAYSSMKNSGMDIIKHYPPVRVWGTIGFIVALWVVSLLGFETSPM
                     QFYIAAVASLALGLYAFTLPKCPPLAKGSKKKLYEELGLNAFKLFKSTKMALFFIFAM
                     LLGAALQLTNAYGDTFLHDFAKVPAYKDLIAVKYPAIIMSVSQISETLFILTIPFFLK
                     RFGIKKVMLMSMVAWVLRFGLFGLGNPAEGLWMIILSCIIYGMAFDFFNISGSLFVET
                     QSTPDIRASAQGLFMMMTNGFGAFLGSRISGIVIDKYFILESGAFDWKGIWFAFAGYA
                     LVIAILFAIFFKHKHVPEETVSVSH"
     gene            complement(11969..12610)
                     /locus_tag="PLA17_02240"
     CDS             complement(11969..12610)
                     /locus_tag="PLA17_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPK84263.1"
                     /translation="MKNIVLFAIMTLITGILSGQPVQDIKLNAPDMERGLTIMKAFEN
                     RASATSFSSRELSLQDLSDLLWASNGINRPESGKRTAPTAVNAQDIDIYVLLRSGAYL
                     YDHKTHTLKGIAAGDNRTLVSDRQTAFADAPVMIVLVSDISRFTVGDDARKLSWGAMD
                     AGIVSQNVSLFCAGAGLVTRPRASMDFEGLKKLLQLNSTRHLMLNNPVGYPNE"
     gene            complement(12671..13582)
                     /locus_tag="PLA17_02245"
     CDS             complement(12671..13582)
                     /locus_tag="PLA17_02245"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPK84264.1"
                     /translation="MKKITFLLLLALLLAGINPAAFGQDADTIQGWQKSGVFSLNVSQ
                     ATFTNWAAGGQNSVALNGLVNLGANFRMGKSAWDNALIIGYGKMIQKGNDLGWVKTDD
                     RIDFQSKYGRKATDKWFYSGLMSFKTQMDNGYNYPDTDNKISGLLSPAYLLFSLGMDY
                     KPNPAFSMFLSPLTSKNTIVMDDDLSTAGAFGVEPGKNFRAELGAYANIAYKNDEVFK
                     NVSFMTKLDLFTNFLKNPQNIDISWEALLVLKVNEYISATVNTLLLYDDDILIKIDED
                     GAGNPLLGKRAQFKEVIGVGFTYKFIK"
     gene            complement(13661..14419)
                     /locus_tag="PLA17_02250"
     CDS             complement(13661..14419)
                     /locus_tag="PLA17_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HPK84265.1"
                     /translation="MTRTRIAALVVSLIMLTGCAELLNTLKTVSAVKQLTEADIIAGL
                     REALSVGARNAGGRLALQDGYWGDPEVRIPLPEEAKAIVENIGKIPGGQQLIDNVLLS
                     INRAAEDAARDVAPIFVNSVMQMTITDGYNILHGADNAATVYLKNTTWNGLYDLYKPK
                     ISASTGKEIVAGVSAQDSWNTLTDKWNQVANSVAGRLAGFKPVNTDLDDFLTRKALEG
                     VFLKLEGEELKIRKEVSARVTPILREVFGTLDQQ"
     gene            complement(14442..14852)
                     /locus_tag="PLA17_02255"
     CDS             complement(14442..14852)
                     /locus_tag="PLA17_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84266.1"
                     /translation="MKRFLTIAIIVLLLVAAGIFWWRHYFVFGEGVKAGDLNFLVRKG
                     YIFKTWEGKLIQTGFRTPTPGAMQSNEFEFSITNDSLATILERASGKFVELRYKEYIA
                     PLPWRGMSKYVIIEILDIRDSEHEHQTLIPYQAI"
     gene            complement(15024..15350)
                     /locus_tag="PLA17_02260"
     CDS             complement(15024..15350)
                     /locus_tag="PLA17_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84267.1"
                     /translation="MENQEKKETYHQKIQEQLDEWRADIDRLKEKAKNAKAESKLKYQ
                     ENIDKLELKMNEGKSKLKDLKESSAEAWDALKDGADSIWDTMKATFAEVREKLTDKDD
                     DDDKKA"
     gene            complement(15570..16469)
                     /locus_tag="PLA17_02265"
     CDS             complement(15570..16469)
                     /locus_tag="PLA17_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84268.1"
                     /translation="MKRSYFILCLALLITAPAIFGQKPIKVLEDSIQFGNYLYPGFNV
                     TIPEASYDKVLKDWIKTQESGTKSKVQTESGEMTIFGANIKEISPAPVNIYSRIMNED
                     TLSRMLVCIELKKDQYVGSASGDLQVTSAKTYLKEFAKKQYMDFIKEELAAEEKILRD
                     LKKDLGSLESSKARSQRKARSNRNTVNAEQEKLLVKHNELSLLSNEIINKNNEVMEMP
                     VGPGRDAMAAQVKELEKRRKKLQNEINKGENRIRKAKSAIDQADRAIPRNEDEQAVMK
                     SRIDAQQAVVQTFIDKLNNVKVY"
     gene            complement(16703..17713)
                     /locus_tag="PLA17_02270"
     CDS             complement(16703..17713)
                     /locus_tag="PLA17_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK84269.1"
                     /translation="MKPIDRTLGFINGIAVDRVPFHPILMRFAARHAGVRYRDFCLSP
                     AHKCMANIRCAEDFRSDWVNTMSDPWAEAEAFGTVLSYPDDDLPKVERHAIEEISDID
                     RMAVLRPGDHRRMLARVEEIETYRRESAGALFICGWVEGPFAAWCDIRDINMAMTDLY
                     EYPVEVHKALDIITESAMAFITPQVRAGAHCIGIGDSVCSLISPELYDEFCFEREKIL
                     VEHIHSLGALAKIHICGNISAILPRVISTGADIIDIDHRTGPVTEVLGLLQPGQVFSG
                     KSDPVSVLQDGDTASISSSCRSFFDEAGGRAILSAGCEVTPGTPVENLRFFSGMAGEL
                     LP"
     assembly_gap    17763..17862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17882..18643)
                     /locus_tag="PLA17_02275"
     CDS             complement(17882..18643)
                     /locus_tag="PLA17_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HPK84270.1"
                     /translation="MLALEWLNNARNVMNNIEETQLENIRKAAEVMADTIECGRWVHT
                     FGCGHATLPIEEMYPRIGGFVGFHPMIEIPLSFFTHITGEMGVHQFVFLERVEGYGVE
                     IMKGYTFDKRDTMWLFSHSGINNVNIDIALESKKRGMKVIVFGSAAAAKGKKTRHSSG
                     KTIFDIADIVVDTCAPLADASVNLRNHHDRIGPVSTMAFVTCVWMTITTVAEILVDRG
                     VKLYIHPSHNVPGDNTARERLDEALAEYKRRIAGV"
     gene            complement(18670..19833)
                     /locus_tag="PLA17_02280"
     CDS             complement(18670..19833)
                     /locus_tag="PLA17_02280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK84271.1"
                     /translation="MKQTYNRRLVFWTACLGMLLFGIGLIMLGSVLPDLRERHSLNAV
                     EAGTLLSLLPFGIIFGSLLFGPVCDRYGYRALLAVSALLMFAGFEGLAFTRTTGILKL
                     SVFLFGLGGGAINGATNAVVADISTTGKGAALSILGVFFGIGALGMPLLLSLLMAKFD
                     FQVIVAATGVLALAAAVLFAFVRFPAAKQAEGISMKQVGSLFRDVTLLLIAFFLFFQS
                     SFEGLINNWTTTYLMDRVAAPQEVALMGLSAFVAGMTVARLLVGSVLRNLPERQLLYI
                     SLGVTFAALLLFSLSRSTVPAVAGLFLLGAGLAAGFPTMLGIVGNRYPDLSGTAFSFV
                     LVIGLVGNMIVNYLMGIVTENAGIKHLTTFTYAELAILVVMATAIFGRLKMNK"
     gene            complement(19848..>20647)
                     /locus_tag="PLA17_02285"
     CDS             complement(19848..>20647)
                     /locus_tag="PLA17_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK84272.1"
                     /translation="VAAAEGEGDPVEAIGISVPGISWRSKGTVWAPNIQGWDDYPLLK
                     EVTNVAGRIPVVIESDRSCYISGEMWKGNARDCRDAIFLAVGTGIGAGIAVNGELLHG
                     ADDIAGAIGWMALSRPFMSKYAICGNFEYFASGEGIARQAADYLNKHDKYQGELRKGQ
                     GMKVTTHDVFAAREKGDVAASAIIDLAVEYWGMAAANLVSLFNPEKIIFGGGVFGPAV
                     ALIPAIYHEAEKWAQPISIRQVSFEPSALGDSAGVYGAGYLAMKQTG"
BASE COUNT         4840 a         5524 c         4835 g         5348 t
ORIGIN      
        1 atctatacag taagagaaag tctctggatg tcagtgcctt ccatagccgg agccctcctg
       61 ggtgcagtga tagcagtgaa gatcaatgaa agagtgatgg agcttctgat agccggactg
      121 cttgtcttca tgttctttgt aatactttac aagcctgaga agtggttgcg tgaccaggct
      181 caatctgcga tcatgcagcg gcggtggtgg gtgccagtgt tgtttttcat catcggcata
      241 tatggcggtt ttatccaggc cggggtgggc tttttcctgc tggcggcgct ggtgctcggg
      301 accggcatgg gcctgaccag ggccaacgcc cataaatcgc tgattgtggc tgtcatgact
      361 gccatatcac tggttgtatt tgtcttcagc aaccagattt attatctcta tggcatcatc
      421 ctggctgtag ggcagggtgc gggagcctgg ctcggctcga aggtggcagt aagctggggc
      481 cctaaagtag tacgtgtaat actgcttgta tgcatcatgg tctcggcaat tgaactcacc
      541 ggactctatg atctggtggc agggtggttc tgaaacagaa gaattctatt tgagacggaa
      601 aacgtaggtg attgtccctt tctgaatgat gggggcatta ttgctgcttt caaacctggt
      661 cttcagcgca gcttcctttg ccagcttgag gaggtccacc tcaagtgtgg tcgatccctt
      721 gatgcctggc gttgcatcag taacccttcc ggaacggtca accgttatcg caacaacaac
      781 aactccctct ttctgcaggt catagggagg tctgatgagt gactgtgcct tccttccccc
      841 gaggtcaaaa gatatgccgt ctgtaccctg cccgcctccc ggcccgtaat ttggtgcatc
      901 aggagtacct gactcaactc cctggttgcc ccgcccgcct gccacaccct ggctctggcc
      961 tgaggtaccc acattgcctg cattgctgaa ggcgcccctg gccctggcat tctgctggtc
     1021 aatgcggcgc tgctcctcct cccgcttccg tctctcggcc tcctcctgtt ccaggcgctt
     1081 ccgctcagcc tcagccctgg cctcagcctc cagttgcctt tttatctctg cctcacgcgc
     1141 ctcagccagc cgtttgatct cctcaggacc gggctctttt ttttctactt ccacagcctc
     1201 ctcgaaatcc tgtgtcaaaa gcgcttcctc acctccctgc tcccatgcag attcaggcgg
     1261 agggggagga gatacaggct ccggtgccgg ttcaaacaat ccttcacctg tctcatcaaa
     1321 tccaaagttg acaagaagac cctcctcaac cggatcaggt atcctcaccc ggaatgtgac
     1381 gaaaatgatg agaatggcaa gaagaaggtg agtcagggcg ctgaagatca agccctgctt
     1441 cctctccttt gtaagaggct cacttgtatt atttcggagt agtggcaaga attagcttga
     1501 actggttttt tgcggctatg ttcatcacgt tgactacata ctctatagga acactcctgt
     1561 cagcatgcac cgaaatgtat atctcgggac tgtcctgaac cctcgacctc aatgcagact
     1621 ccaggaattc aagatcgatg ggctgatctt caatgtaata ctgaagatcc tttgtaattg
     1681 atactgtggt catcggacgg gctgaggact gggtcgtacc cttgggcaac agcagcttca
     1741 gggctgtcgg gtgaaccagg gtagaggtca gcatgaagaa cagcagaagc aggaacacga
     1801 tatcagacat gcctgcattg ctgaactgaa tactgacttt attcttgttc gtaattgcca
     1861 tcagttactc ttttacaggt tcattgagga tatcgaggaa ctcagttgag gttgtttcaa
     1921 gctggaacac tacattttca accttgacaa ccagctggtt gtacccgaaa taagcgagaa
     1981 tcccaagtat cagccccccc accgtggtaa ttagcgcggt atagatacct ccgctgaggg
     2041 tgcttatgtc aaggctgctt cctgccagtg acatttcata gaatgcctgc accataccta
     2101 tcaccgttcc aaagaatccc atcaggggag cggctccggc aattgttgcc agtgtcggga
     2161 acctcttctc aagcttgtga acctccagcc tgcccacatt ctctatggca accgtcacat
     2221 cggccagagg cctgccgaga cgggtgatcc ccttctcaac catccgggcc tgcggtgagt
     2281 cggtcctcct gcagagctgc aacgccgcat caaccttgcc ttcatacatg tagtccttta
     2341 tgcggttcat gaagttggca tcactctttg atgccttctt gatcaccaga tacctctcaa
     2401 taaaaatgta aagtgcaaca aaggagagca gtgtgatgag atacatcacc catcctcctg
     2461 ccaggtaaag atcagccagt gacattgttg cctcttttgt gacttcagtg gcttcagtga
     2521 ctggtgttcc ctgtaataaa atagctgctg atatcatttt tcttggttta agatgttaac
     2581 agtttgactt gctttggcgt atcatcgcgc gaatatagta aaaaacggag ttcatcccct
     2641 gtaagtatcc tgttgttgat aaaacgggtt cattgttgaa atattaatcc ttccctgact
     2701 ccccgtcacc attgaccttt ttcttcttct catccggtct gatgaaaagg tctttcagcg
     2761 tgctgaaatc gcgtctgaaa aaaagtccca ccccctgggt gtaaggatga acctggtaat
     2821 agaggttgtg attcgaacga ttgaatacct tgaacctgag catatcggta agtctgacct
     2881 ccaccgtgaa ctcgccggta atattcttgg tgtaggtgtt tgactggttt ccccgcacgt
     2941 ctacgttccc gttgacggta accttgtcat tcagcaactg cgttgagaga gccagctcca
     3001 cctcctggtc ggtgagctcg ctcccgggtc tgtagctgaa gcctatgtca aaatcattgc
     3061 ttatctgtga cagccagttg ctgacctggt tggaaagcat ctcggtagtg gttgtgacgc
     3121 cacctgcaat tggtgatgcg gcatatcccc cctgagccaa cggtgtgcct gtggcagcgc
     3181 ctgaattgta aagtgaagga tcagggcaaa agctgttcat taccagcagg taaagaaact
     3241 ggcgcctgag ctcctcctct gtgtcaatgg ccagtctcag cagctcacgg gtgcgctcgt
     3301 cagcggtagg gagactgatg tcaaagctga ttcccggatt caggagcctt cccgagagac
     3361 tgagaacgca ttcgaccggc atcttggcct taagctcggt gaattcctcc tccccgaata
     3421 tctccgagag tgatgccttg gtcttgtaca gggccctgat atccaggctg gcatcttcaa
     3481 tggccccgtt ccacgagact gtccctccct tttccactgc aaacctcttg ttgagtatat
     3541 ttccaagagt gaagaggtaa tctccttcac ttatcacata gtctccggcc atacgtatgt
     3601 caccccttga gttcagtgag atgttcagct tgccttcacc ttttcccctg atgacacctc
     3661 cggatgtttc gtcaaggata agctgcacct cggcttcagg cgatacgtca aggtcgaagt
     3721 tcagctcgat cttgcttccc tcttcctgcc tcacaaaact gccatgcctg tcagcctgct
     3781 cctgcttacc ggtctccccg gcaggtttca aaaactcaat gaagggatag tcacttactt
     3841 tggccgatga gttcagcggc acaaagaatt cagtgtccgg agccgtccgg gccgagatat
     3901 tgaaaaccag cttctcatca ttacccctta tccctgcata acctgaagca tatgcggtgc
     3961 cgtaaaaact gtcattgtcc tttggcctgg tgttgagaac cagcattttg tccatattta
     4021 tgtcaaggct cacaccaaaa ttcctgaatg cacggtgcgt gatcataccg ttgatcatgc
     4081 cctgattttt ctgtgtatca taaatattga tattcctcag gatgatgcct gcaggcgaaa
     4141 accttacgct gtcagagaaa ctgtatcggg tctgaagaaa atcgatcttc attgaggcat
     4201 catgcgccat tactgctccg gtcatctcca gttgccgcaa ctttccattc agcctgatgg
     4261 ttcccgttcc gaaacccctg atatccgaag caaaagagct gagaaacgga ttgagaacat
     4321 caagcggcag atcagatgcc gaagcagaaa tgtcaattac acgtgccgtg ggcgaatagg
     4381 ttccgctgac atcaatgagc ttcttcccgg caaagtcatt gcccatattg attaccgcca
     4441 cctttcttcc cggatcccat tcgctgataa tggtcataag gccatagttg ttgtcgttca
     4501 gcctgaagtc agtgatattt atgctgcttt caaaaagtag attattgtaa acatctgaca
     4561 gggtgagatt gccgctcata gtcccttcaa acttcatctg ggcaggtttc tttgtgtcct
     4621 gcttcccctg gaggaggttg ttcagatatg acatgttgag tccctcgaac gtcagggtca
     4681 gcttctcatc ggggtctttc gagagcctgc ctcccagact gaaatatttt gtcctgctgc
     4741 ttacaagtat attgtcgaaa taggcgctga cagaatcaat tacgatcctg gcaggagtga
     4801 tattccactg tgagctgttg acagtcagac tggcaggaag cacggcaacc gtcagtgatg
     4861 gcaggttgcc ggcactgtga ccgaagaatc ccctgactct gatctctccc agggtacgcc
     4921 cgttgtcatg attatcccag ttcatgccca ggtcaattgt gtcattgctg cttatggcac
     4981 ccagggcgaa atttcccagc tccgaattgt caggcagcgt cactgtatct gctgccaggt
     5041 ccagcttcat gctgccattt tgtaacgaac ctgtcatgtt cagacggccc attcgtacac
     5101 ccaggtatgt taccgcatct gcctcaatcc cggcaatgac ctccggacgg tctgaccaga
     5161 agtgaccagt gataagcgtt ccttccgaga tactgaggcc ggtcccgaaa aactcattca
     5221 gtctgtcaat cctcttgatc ctggtatcca gggagaacat cgcctcggat gttgactcac
     5281 cctttctggc tacaggttta ctgaacctcg aagggaaaag gtctgccttg atctctgcga
     5341 cgctcctttt tatggcatca acggagtatt ttcccctcac atcagcatcg gcaaaatctg
     5401 acctgagctt caggaaaggc acaccgccga cgatttcgga actgacaagg aaatcatata
     5461 tgttgagctt cccgtttgag ttctcaagag tcgagttgat aagcctcagg tctccttcca
     5521 ggttttccat tgagttgccc ctgaaattgg cagtgagaag cgccgtggcc ctgaagatgg
     5581 aatctttcgt cacaaggttc agagtgtgca ggtctgcact ggcaaggttc atggtgaaat
     5641 caaactccgg cttgctcttt tcaagatcaa acctgccgag aaggctcatc ctgatatttg
     5701 gctcattcac ctcaacactg ccatcccaga tcttgtttgc ataggtccct gatacagtga
     5761 tgttcctgta taggtatttg tttatctcta cactgtctat caccccgttg atgtttgccg
     5821 agagatggcg gaatgacttc attgttccgt caacctctgc atgcatccac agcccgccga
     5881 acatctcact cttttttgtc atgtacccga gatcaacagc actggttctg agagaacccc
     5941 tgaatccgaa ggcgtttcgc ccgtcaggct taagcgagag gtctgtgtca aacgtacccc
     6001 tttcagtggt gaatctgccg aaagagacaa agtcagtggt gaatcctgta aagcttccct
     6061 tgtaggtgac cagccctatg tcatgagcaa ctgctggcag cctgaacggc ttcctgcccg
     6121 gaagggtgaa ccgttcgata tctgcagcac gggttttcat gtcggtgaaa ctgatgtaca
     6181 gataactgtc gtttatctca ggaagtccgg aaatgtcaaa atcgaaccgc agcctggtgg
     6241 agctctcata gtcaatcctg atattcttcc ctttcatctc tgcaacggtg ccggtcaccc
     6301 tgcctgagaa actgaccttc tcactgaccc cgttaagggg cctgacaaaa tatgagatgt
     6361 cagaggtatt tacgatggaa ttatcaaaca ggaagtcaag ccttacatca ttgatgaaat
     6421 ccttccatga atctttttca cggggcataa tcaggatctt ttcagcagaa atggcgcttg
     6481 agtcggtgag caggtcaaaa tccctgaagt agaggtcgct ctcagccacc gccatattca
     6541 tatcaagact cctgctgata aatccgccgt cttcttgaaa cgccagcccc cttatcacca
     6601 tgcttacaga gtcgggtatt accctgagat cattgacttt tccggtgata tgcctgatat
     6661 acatgttcct gtagttgacg ccacccggta taaccccgac ggtgtcagac atgtctgaca
     6721 gtgtaaagga tccgttcagc aggtcaatat cagcaaaatg tatctcaatg ttccttgtgc
     6781 tgtcacgctc ccggtcactc ttcagtgact ggagataggc agtcagattc atgaccccgg
     6841 cagtgtccct gatcatcctg aaatcgggct cgtgaaggtc aatggttctg aatttgtaaa
     6901 tcttttgtga tggtctgatc tcacgcagcc tcatatcaac cttgcgggca gcaatgatgg
     6961 tatcaccgtt caggtccttc aacagtacat catcaagaac aatctcatgc cacagtgagt
     7021 atgaaaccct gcctatcatg atctcagctc ccgtttccct ggagaccgcc tttgtcactc
     7081 tgtttaccgt ccaggtctgg atcagcggcg tctgaagcag caggaagaga atcagcggca
     7141 ccacaaccag cactgctgtt atgatgagaa ggcccctgat gattttttta ataattttgg
     7201 ccattcacga agagattccc gccgctggcg aagttataaa aaagagtgat acaaaaatga
     7261 caccacaaat tacgatcctc gcgatagaat catcctgcga tgacacctct gccgcagtga
     7321 tctgcgacgg attcgtactt tcaaacctga tcgcaaacca ggatgttcat aaggcttatg
     7381 ggggtgtggt gcccgaactt gcctcaagag cccaccaggt aaacatagtc cctgtggcag
     7441 atcaggcact tgccagggcc gggaaaagag ctgacgaggt aagcgcagtg gccttcacac
     7501 agggaccggg attgctcggt tcactcctgg tcggaacatc atttgccaaa ggttttgcgc
     7561 tggcgcgaaa catccccctc attgctgtaa atcacctcca ggcacatgtc atgtcgcact
     7621 tcgccatgga acacggaagg gaaaaccgtg tcccgcaatt ccccttcctg tgcctgctgg
     7681 tgtcaggcgg ccatacacaa ctgatcgtct tgcgtgatca tctggaaatg gaaatcatag
     7741 gcaataccat agatgatgca gcaggcgagg catttgacaa gtgcgccaag gtgatgggac
     7801 tcccttaccc gggcggaccg gagatcgacc ggcttgcaac agacggcaac cctgacgcat
     7861 tccgtttcgc aaaaccgcgg atacagggac tgaatttcag tttctccgga ctgaagacaa
     7921 gctttctcta tttcctgcgc gacagggtgg ctgaggaccc cggattcatc gggaagaatc
     7981 tcaatgacct tgctgcctca ctgcagagga ccatcattga gataatcatt gggaagctgg
     8041 tgagggcttc cagagagacc ggcatcaggg agatcggaat tgcaggaggg gtctctgcca
     8101 acagtggcct gagacgtgct gcgacgcgtg aagctgaaaa aagaggctgg aatcttttcc
     8161 tccccgaact caggtatact acggataacg ccgcaatgat cggtattaca ggatactaca
     8221 agtacctcaa ggcagaattc actgaccatt cggtgattcc ggcggctcgg attgatactt
     8281 gaacgcctcc ctgccagggt aatggtaccg ggcacgggca accttctcag gataaatgaa
     8341 atagtcgagc tcagcctcag cacagagctt cccttcaccg tcgtgaagcg ctgctgagat
     8401 caacgcgctc ttttcttccc tcttcgttat catagcctca acggttacag gtcctttgct
     8461 gatatgtacc ggtttgagat acctgaccgt cattgaagag gtaacgccgg ctgtacaggc
     8521 cttgagatag acacaccatg cgctgacctc atcgagaagg gtggcaatga tgcctccgtg
     8581 cagcacattg atataaccct ggtactgcct gtccggcatc catgaggcat ataccttctc
     8641 atcatcctcc tcaaacaaca gtttcagccc tatttggttt ttcggaccgc atccgaaaca
     8701 ggtgttctgg tcagcatcaa acggattaat tatttttctt ctgcgctcca tcttcagttt
     8761 ttctcctctt tttcttttgc cgggcaatcc atgcactcag gtaaaatatg gaaatgatca
     8821 aatatgaaaa gcctatgatc agtgatatgg cagcaagcgt cattgacggt tcctcctttg
     8881 gcgtatcggc aattctcata cgataaatgt acccgtagac cgttgtgact atgaccattg
     8941 ttgccagaat gagtcccaag aagacaatgg tgccgtcaat ggctgtcgta ctcctgtaaa
     9001 ccgggctttt ccggaattcc gcgtatgagg cttttgcata agcctctgca cgcctgcgcg
     9061 cttcctccct ccggtaatca atccatgcct ggtaattgcg cattatctct tcctcagtga
     9121 tattccgccg gtagggatga gacatcagat actcataggc ctcggtaacg cggatgaaca
     9181 tctcctggga atcaggcgaa ctgttccggt caggatggta ctccattgcc tttctcctgt
     9241 atgccttctt tatctcatca tcagtggcat tctgcttaat gccgagtatc ctatagtaat
     9301 cctggagatt catgactcaa tgcaatatca aaataaggat ttttttcagt agaaatactc
     9361 ctcatgttgt ttttcctgtt attcttttac ttttgcaatt taaactcatt ctaaataacg
     9421 tctcctgatg gatagttccc ggctactctt tgcatttgga atcactctct ttgctggcct
     9481 tgcaaccggg ataggcggtg tgatagcttt caccgccaga cgtaccaata ccacatttct
     9541 tgctttttcg cttggattct cagccggagt tatgatcttc atctcattca ctgagatact
     9601 taccgaagct ggtgacatca tgaggctgaa ttatgaagcc aatactgcag cctggctgac
     9661 atttatctct tttgtggcag gcattggcct ctctgccgcc attgacaaga ttcttccatc
     9721 agagggtaac ccccatgaac tgaagagggt agaacagatg catcccgaat ccctcaaccc
     9781 ggataaacct gaatgcgaaa ggagaaagaa ccggaaccgc ggaggccact gcattggaga
     9841 ccggaaactg atgcgcatag gaacatttac tgccattgcc attggtatcc ataacttccc
     9901 cgaagggatt gccacattca tgtcagccat gtctgatgtg gctgtaggta tcagcattgc
     9961 agtggctgtg gctattcata atattccgga gggtatcgcc gtgtcggtgc ctgtctatta
    10021 tgccaccggc tcaaaaagga aggcgcttgc atggtcagta ctgtcaggat tctcggagcc
    10081 gattggcgca ctggtaggat atttcattct gaggctcttc aataccagca taagcctcgg
    10141 ctatgtattt gcaatggtgg ccggtataat ggtctatatc tcctttgatg aactcctccc
    10201 ggctgcccac aagtatggca aacaccatgt cgcaatctat ggcctggtaa gcgggatgat
    10261 aataatcgga atcagcctga tcctgctcgg gtaaccacac cggcccgtag cctctccatg
    10321 ccctggtcag ctgtccggca gccgtgcctg cgcctgccag ggtaaccaca ccggcccgta
    10381 gcttctccat gccctggtca gctgtccggc agccgtgcct gccagggtaa ccacaccggc
    10441 ccggagcctc tccgtgcctg tgcctgccag ggtaaccaca ccggcccgga gcctctccgt
    10501 gcctgcgcct gccccacacg gggcaaaaaa tcagtggctt actgaaactg tctcctccgg
    10561 cacatgtttg tgcttgaaga atatggcgaa cagtattgca atcaccagcg catatcccgc
    10621 aaatgcaaac catattccct tccagtcaaa tgctccgctc tccaggatga agtatttgtc
    10681 aatcacaatt ccgcttatgc ggctgccaag gaatgccccg aagccgttgg tcatcatcat
    10741 gaagagtccc tgcgcgcttg cacggatgtc aggagtgctc tgggtttcca cgaagagcga
    10801 accggaaatg ttgaagaaat caaatgccat cccgtaaatg atgcacgaga ggattatcat
    10861 ccagagccct tctgccggat tgccaaggcc gaacagcccg aaacggagca cccatgccac
    10921 catgctcatc agcatgacct tctttatccc gaaccgcttc aggaaaaatg gaatggtcag
    10981 tataaaaagg gtctccgata tctgtgacac agacataatt atggcagggt atttcactgc
    11041 aataagatcc ttgtaggcag gtacctttgc aaaatcatgc aggaatgtgt ctccgtacgc
    11101 attggtgagc tgcagcgcag cacccagcaa catggcgaag atgaagaaga gagccatctt
    11161 ggtgcttttg aaaagcttga atgcattgag tccaagctcc tcataaagct tcttcttact
    11221 gccttttgcc aacggaggac acttgggaag ggtgaaggcg taaagcccaa gtgcaagcga
    11281 agcgacagca gctatgtaaa actgcatggg tgaggtttca aatcccagca gactcaccac
    11341 ccaaagcgcc acaatgaaac cgatggtgcc ccagaccctc acgggcggat aatgctttat
    11401 gatatccatc ccgctgttct tcatcgagct gtatgccaca gtaatggcca gggccagtgt
    11461 cggcatgtaa aacatcatgg tgagaagcag cagccagaag aaggtatcag gatcgtttac
    11521 agcaggtatc agtgagagaa gaattgcccc tgcaatgtga aaaattccat acagcttctc
    11581 cgcgttgatc cacctgtcag ctataatacc cgcaattgcc ggcatgaaaa gggaggccag
    11641 acccattgtt gaaaagatgg ccccgaaatc ggtccctgac cagtttttat tctgaaacca
    11701 gtatgcccct atggtcagaa gccatgaccc ccagatgaag aactggagga agttcattat
    11761 gataagccgg ttcttgagtc ccataatcct gttttttact ttgaatctgt gaatatagta
    11821 aactatactg ttccggataa ccagtctcat gcatcacccg gccaggtgac acaatacccg
    11881 tttttattca gatggctgtc agcgttgtca atcaaatatc cgcgcagggg gagagatgat
    11941 ctgtcagctt aaatatgcca gagaaccact actcgttggg ataaccgaca ggattgttga
    12001 gcatcagatg ccgggtgctg ttcagctgga gcagcttctt cagcccctca aagtccatcg
    12061 atgcacgcgg acgggtaacc agtcctgccc cggcacagaa gagagataca ttctgtgata
    12121 ctatccctgc atccattgca ccccagctca gcttgcgggc atcatcacca accgtgaagc
    12181 gcgatatgtc agagaccagc acaatcataa ccggtgcatc agcaaaagcg gtctgcctgt
    12241 ctgaaaccag cgtcctgttg tcaccggcag ctattccctt aagagtatga gtcttatgat
    12301 catacaggta agctcccgac ctgagcagca cataaatgtc aatatcctgt gcattgaccg
    12361 cagtgggagc agtgcgcttg ccgctttccg gcctgttaat gccgtttgat gcccagagca
    12421 ggtcagagag atcctgcagg gagagctccc tggaactaaa ggaggtggcc gaagccctgt
    12481 tttcaaatgc cttcatgatt gtcagccctc tctccatgtc aggggcattc agcttgatgt
    12541 cctgcacagg ctgacctgac agaattcccg taattagtgt cataattgca aaaagtacta
    12601 tgtttttcat actgctttgt gattaacaat ttagcaaaaa cttaccggcc tgttactatt
    12661 aaggtgtctt ctatttaatg aacttgtagg taaaaccaac gccgatgacc tccttgaact
    12721 gtgcgcgctt gcccagtaga gggttgcctg caccatcctc gtcaatcttg atgagtatgt
    12781 catcatcgta gagcaggaga gtgttgacag ttgcagagat gtattcattg accttgagaa
    12841 ccagcagcgc ttcccagctg atgtcaatat tctgcgggtt cttcaggaag tttgtaaaga
    12901 ggtcaagctt tgtcatgaaa ctgacgttct taaaaacctc gtcattcttg taggctatgt
    12961 ttgcatatgc accgagctcc gctctgaaat tctttccggg ttccactccg aaagcacccg
    13021 ccgtggaaag atcatcatcc atgacaatgg tattctttga tgtcagcggt gagagaaaca
    13081 tcgagaaagc cggattgggc ttgtagtcca tacccagtga gaacagcagg taggccggcg
    13141 agagcagacc cgatatcttg ttatccgtat ccgggtaatt gtatccgttg tccatctgcg
    13201 ttttgaaact catcagaccc gaatagaacc acttgtcagt tgccttccga ccgtacttcg
    13261 actgaaagtc gatacggtca tcagtcttga cccacccgag gtcattccct ttttgaatca
    13321 tctttccgta accgatgata agggcattgt cccatgccga cttgcccatc ctgaagtttg
    13381 ctccaagatt aaccagtccg ttcagcgcca ctgagttctg gcctccggca gcccagttgg
    13441 taaacgttgc ctgtgacacg ttaagggaga atactccgct cttctgccat ccctggatgg
    13501 tgtcggcatc ctggccaaat gccgccgggt ttattcctgc aagtagcagt gcaagtaaaa
    13561 gtaaaaaagt tattttcttc attaatatca gattttgtgt gcaaatataa caacagaggc
    13621 ataaacttgt tgagcccgct tcaaacagtc cggaggtgtc ttattgctgg tccagcgtcc
    13681 cgaatacttc cctgagtatg ggagtgaccc tggccgaaac ctcttttctg atcttcagtt
    13741 cctcaccttc aagcttgagg aagaccccct cgagtgcttt acgtgtcagg aaatcgtcaa
    13801 gatcggtatt caccggtttg aatccggcca gccttcctgc cacactgttg gccacctgat
    13861 tccacttatc cgtaagggtg ttccatgaat cctgtgctga gacacctgcc acaatctcct
    13921 tgccggttga tgcgctgatc ttaggcttgt agagatcata aagcccattc catgttgtgt
    13981 ttttcagata aacggtggca gcattgtcag cgccgtggag gatattgtac ccgtcagtga
    14041 tggtcatctg catcaccgaa ttcacaaaga tgggagcaac atcgcgggca gcatcctcag
    14101 cagccctgtt gatgctcagc agcacattgt caatcaactg ctgtcccccg ggtatttttc
    14161 caatattctc aactattgcc ttcgcctctt caggcagtgg tatcctgact tccggatctc
    14221 cccagtatcc gtcctgcaat gccagtcgcc cgcctgcatt gcgcgctccc acgctgagag
    14281 cttccctgag ccccgctatg atatctgctt ccgtaagctg tttcaccgct gaaaccgtct
    14341 tcagcgtgtt cagcaattca gcacatccgg tcagcataat aagcgataca accagtgctg
    14401 caattcttgt tctggtcata agtttttctc ttttgttcgt ttcagattgc ctggtagggt
    14461 atgagcgtct gatgctcatg ttccgaatcc cttatgtcaa ggatctctat aatcacatat
    14521 ttgctcatcc ccctccatgg aagcggtgcg atgtactcct tgtaacggag ctcgacgaat
    14581 ttgccgctgg ctctttcaag tatcgtggcc agactgtcat tcgtaatgct gaactcgaac
    14641 tcattgctct gcatggctcc gggagtcggt gtcctgaaac ctgtctgtat cagcttgcct
    14701 tcccaggtct tgaaaatata gcctttcctt acaaggaagt tcaggtctcc ggccttaacc
    14761 ccctctccga atacaaagta atgacgccac cagaagatac cggcagcaac aagcagcagt
    14821 acaataattg ctattgttaa aaatcttttc atcttttttg cgattgttaa atcatatggg
    14881 ggtttatgtt tatcagtaca aaactatctg atcccggtag tctgcaatca gtaaccatac
    14941 aataccacaa catgctgata aacaacaaat tgatcgtagg ctcactactg tctgctgccg
    15001 acggccgggt caggacgcac tcctcacgct ttcttatcgt cgtcgtcgtc cttgtccgtc
    15061 agcttctccc tgacttcggc aaaggttgcc ttcatggtat cccatattga atcagctcca
    15121 tccttcaggg catcccatgc ctcagcactt gattccttga ggtctttcag tttcgacttc
    15181 ccttcgttca tcttgagctc aagcttgtca atgttttcct ggtatttgag ctttgattcg
    15241 gctttggcat tctttgcctt ttctttcagt ctgtcaatat ctgccctcca ttcgtccagc
    15301 tgttcctgaa tcttctggtg gtaggtttcc tttttctcct ggttttccat aattgattta
    15361 atattttggt tcattaatgg gtattagaaa atactatgat ggaataatcg catgccaggc
    15421 atctctttgt gtatggagtg cctgtcacag agatgcttac aggatgcgat tacgctaatg
    15481 tcaaagagct taaaaaaacc agttaacaat cagtgggttt caagtgcccg ctggttatac
    15541 tggttataaa ctaatactgt ccgtaaggat cagtagactt tgacattgtt cagcttgtca
    15601 atgaaggtct gaacaacagc ctgctgtgca tcaatccttg atttcatcac agcctgttca
    15661 tcttcattcc tggggatagc cctgtcagcc tgatcaatgg ctgatttagc cttcctgata
    15721 cggttctcac ccttgttgat ctcattctgg agcttcttgc gccttttctc gagttccttc
    15781 acctgggcgg ccatggcatc cctgccggga cctaccggca tttccatcac ctcgttgttc
    15841 ttgtttatga tctcatttga cagaaggctc agttcattat gtttgacaag cagcttctcc
    15901 tgttcggcgt tcacggtgtt cctgttgctt ctggccttcc tttggctacg ggccttgctg
    15961 ctctccaggc ttccaaggtc ctttttgaga tctctcagta tcttctcctc ggcggcaagc
    16021 tcctccttta tgaagtccat gtactgcttc ttcgcgaact ctttaaggta agtctttgct
    16081 gaagtcacct gaaggtcacc tgaggcagat cccacatact ggtccttctt gagctctata
    16141 cataccagca tcctgctgag ggtatcctca ttcattatcc tgctgtagat attgaccggg
    16201 gcaggggaga tctccttgat attggccccg aaaatggtca tctctccgct ctcagtctgt
    16261 accttcgact tggtaccgct ttcctgggtc tttatccagt ccttgagaac cttgtcatag
    16321 ctggcttcgg gaatggtaac attgaacccc ggataaaggt agttgccaaa ctgaatgcta
    16381 tcctcaagca ccttgatcgg tttctgtccg aaaattgccg gcgccgtaat caacagtgcc
    16441 aggcataaaa tgaaatagga tcgtttcatc tggatttagt ttagatcaag gttcaaagat
    16501 actatctttc agagaaaatc tacaattttt acctgtaaaa gtgaaggcct gacagcacat
    16561 cacggtaatt gttccttgcc gggcagaggg cgaactgctg ccggttagtt gttgccctga
    16621 cagcacatca cggcaattgt tcccacggag ccggtgcggt ggcacgctca ctctccctga
    16681 ttatctcagc cgccttgtaa aatcatggga gaagctcccc ggccatcccg gagaaaaagc
    16741 gcaggttctc caccggcgta ccgggcgtta cctcacaccc ggccgagagg attgcacggc
    16801 ctcctgcctc atcaaagaat gaacggcaac tgctgctgat agatgccgtg tcaccgtcct
    16861 gtaacaccga aaccggatcg cttttgccgg aaaaaacctg tcccggctgc aataaaccaa
    16921 gcacttccgt caccggtcct gtccggtgat cgatatctat tatgtctgcc ccggtactga
    16981 ttacccgcgg aaggatagcc gatatgttcc cgcagatgtg gatctttgcc agcgctccca
    17041 gtgaatgtat gtgttccacc aggatcttct cccgttcgaa gcagaactca tcatacagct
    17101 ccggggagat gagtgagcag accgaatcac cgataccgat gcagtgagcc ccggccctga
    17161 cctgcggggt aatgaaagcc atggcgcttt cagtaataat atccagcgct ttatgaactt
    17221 caaccggata ctcgtaaagg tcagtcatcg ccatattgat atccctgatg tcacaccagg
    17281 ccgcaaatgg accctcaacc cagccgcaaa tgaacagtgc gccggcactc tctctccggt
    17341 atgtctctat ctcctccaca cgggccagca tccgccggtg gtcacccgga cggagcacgg
    17401 ccatcctgtc aatgtcagat atctcttcta tcgcatgacg ctccaccttc ggcagatcat
    17461 catcagggta ggagaggacc gtcccgaagg cttcagcttc tgcccatggg tcagacatgg
    17521 tgtttaccca gtcagacctg aaatcttcag cacacctgat attggccatg cacttgtgag
    17581 ccgggctgag acagaagtcc cggtaccgca ctcctgcgtg acgcgctgca aaacgcatca
    17641 ggatgggatg gaagggcacc ctgtcgacgg caatgccatt tatgaatcct aaagtcctgt
    17701 caatcggctt catggtgata tacttttata tagtactgcc aatcggcttc atggtgatat
    17761 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcttcatg ccgtttaatt
    17881 gtcatacccc tgcaatcctg cgcttgtact ctgccagggc ctcatcaagc ctctccctgg
    17941 cagtgttgtc acccggtacg ttatgactcg ggtgtatgta gagtttaact cccctgtcaa
    18001 ccagtatctc cgcaaccgtg gtgattgtca tccagacaca ggtgacaaaa gccattgtgg
    18061 agacggggcc tatcctgtca tgatgattcc tgagattaac tgaggcatcg gcaagtggtg
    18121 cacaggtgtc aaccactatg tcggctatgt caaagatggt ctttccggag gaatgcctcg
    18181 tcttctttcc cttcgcggca gcggcagatc cgaagacaat caccttcatg ccccttttct
    18241 tcgattccag ggcaatgtcg atgttcacgt tgttgatccc cgaatgggag aaaagccaca
    18301 tggtgtcacg cttgtcgaag gtatatccct tcatgatctc aacgccataa ccctcaactc
    18361 tctctaggaa tacgaactgg tgaacaccca tctctccggt tatgtgggta aagaaactga
    18421 gcgggatctc aatcatcgga tgaaaaccca caaagccgcc aatcctcgga tacatctcct
    18481 caatgggcag tgtggcatgg ccgcatccaa aggtatgaac ccaacgtccg cattcaatgg
    18541 tatcagccat cacctctgca gccttgcgga tattctccag ttgtgtctcc tcaatgttat
    18601 tcattacatt tctggcatta ttcagccatt caagtgctag cataattgtt ctgtttgatt
    18661 gttggtaatt tatttattca tcttgagtct gccaaagata gccgttgcca tgaccaccag
    18721 gattgccagc tctgcatatg tgaatgtggt aagatgctta ataccggcat tttcagtcac
    18781 tatccccatc aggtagttaa caatcatgtt gccaactaac cctatcacaa ggacgaagct
    18841 gaaggcggta cccgacagat cgggatacct gttccctaca attcccagca tggtgggaaa
    18901 ccctgccgcc agtccggcgc cgagcaggaa gaggccggcc actgccggca ccgtggagcg
    18961 gctgaggctg aaaagaagca aagctgcaaa ggtgacgccc agggagatat agagaagttg
    19021 cctttcaggc aaattcctca gcacagaccc taccagcagc cgtgccaccg tcataccggc
    19081 cacaaaggcc gaaagcccca tcagtgcaac ctcctgcggc gcagcaaccc tgtccatgag
    19141 gtaggtggtg gtccagttgt tgataagccc ctcgaagctg ctctggaaga agagaaagaa
    19201 tgctatcagc agaagggtga catccctgaa gagggagcca acctgcttca tcgaaatacc
    19261 ctccgcctgt ttggctgcag ggaaccttac aaatgcgaag agcactgctg cagccagtgc
    19321 cagcactccc gtggcggcca caatgacctg gaaatcaaat ttggccatca ggagactgag
    19381 cagcagcggc atgcccaatg ccccgatacc gaagaatacg ccaaggatgc tcagcgccgc
    19441 acccttgcct gtggtgctga tgtctgccac cactgcattt gtggctccgt tgatggctcc
    19501 cccgcccaga ccgaagagaa agactgacag tttcagtata cctgtggtgc gggtaaaggc
    19561 aagcccctcg aatccggcaa acatcagcaa cgctgacact gcaagaagcg ccctgtagcc
    19621 gtaccggtca cacaccggcc cgaagagaag cgatccgaaa ataatcccga agggaagaag
    19681 tgacagcaga gtgccggcct ccaccgcatt gagcgaatgt ctctccctca ggtcaggtag
    19741 cacggaacca agcatgatca gtccgatacc gaaaagaagc atgcccaggc atgctgtcca
    19801 gaatacgagg cgtctgttat aagtctgttt catggttatg gagatggtta acctgtctgt
    19861 ttcatggcaa gatatcctgc accgtaaaca ccggcgctat cgcccagtgc cgagggctca
    19921 aaacttacct gccgtatgct tatcggctga gcccacttct cagcttcatg gtaaatggca
    19981 ggaataagtg caacggcggg gccgaaaaca cctccgccaa atattatttt ttcaggattg
    20041 aacagactga ccaggtttgc tgcggccata ccccagtatt ccacggccag gtcaatgatc
    20101 gccgaggctg ccacatcacc cttctcccgg gcagcgaaca catcatgcgt ggtgaccttc
    20161 ataccttgac cttttctcag ctctccctgg tatttgtcat gcttattcag atagtctgca
    20221 gcctgacggg ctatgccttc acctgaggcg aaatactcaa agttgccaca gatggcgtac
    20281 ttgctcatga agggtcttga cagtgccatc cagcctattg cccctgcgat atcatcagcg
    20341 ccgtgcaaca attcgccgtt tacggcaata cctgcaccta ttcccgttcc aacggccagg
    20401 aagatcgcat cacggcagtc gcgggcattg cctttccaca tctcacccga gatatagcat
    20461 gacctgtcac tctcgataac aacaggtatc cggcctgcaa catttgtaac ctccttcagc
    20521 agggggtagt catcccaccc ttgaatattg ggggcccata ccgtaccctt cgagcgccag
    20581 ctgatgcctg gcactgatat gcctattgcc tccaccggat caccctcacc ctcggcagca
    20641 gcaacct
//
LOCUS       DAOPBK010000021        20206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2783_length_20206_cov_5.194978, whole genome shotgun sequence.
ACCESSION   DAOPBK010000021 DAOPBK010000000
VERSION     DAOPBK010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20206
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_20206_cov_5.194978"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3571)
                     /locus_tag="PLA17_02290"
     CDS             complement(<1..3571)
                     /locus_tag="PLA17_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK84273.1"
                     /translation="MAEKLKNIRIVGVALLITVALAAVADLLYFSDLEWRYRTARADA
                     RLKSLENYAGELLRTAEEMISEAGDSSVLFHSTLGSDALKKGITLLVYHERRIAYWSD
                     NSIAFPVIYEEHFDEHKPVFYSNGWFIPVYLENSEYEMVALIKVYRQYPIVNDILQTG
                     FPETFSLPDGVKITFDPSASDFIIWGSEEEYHFGLIFPETKPNTIFLAIPALLWLIVL
                     FLLVRLVLLVARHIDMKSRTPSGIVFAFAALLAVYGIILLTGLPRSVTSTGLFSPFNW
                     SAGRLIPTVGHVLLLGILVLSGIRFTFRSDRFSKPWSGKGIWKYLVPGVILAMGYVIF
                     LLVEAHFRDLVVNSAISFEAFRILDMTFMSLAGIVSVMLLLTIPVILIMYAARMMHGW
                     SSREILAVAGGITLLLPLACLADTGCNGWSILWILVMITTAFIWQRKSYSGVSLAIIY
                     SVITAMLSTALIIKYSIIREERNMKVMAVSLASDNDMVAEGMIIDMWPALESDTVLAR
                     LVKKEFITPGDLNSVYRYLEDGYFYGYWQNYDISIVICRNDSPLSLGSTGAEVANCFD
                     WFDNRIMNEGDTITNTGFYFMRNQAGRAWYLSKLLYEISPGVTNGLFIELVSHIETFL
                     AGYPELLLDGGYQRFPMLKDISYAKYNKGVLVLRSGEYPYDASIPGGQPEREEFRFED
                     GRDYKHLYYTLGDMTLAVTVTRVKFLDRIITFAYLFIANLTYVIILLVIFTMKPAELL
                     KTDTFRRRLQLSFAAVLTMVFIIVITGALILSTRQFRSNHLRIISEKTASLAIELDHK
                     LSTEKTLDRGWQTPDYPTLNHLLVKFSNVFFTDINLYSPSGRLIATSRPEVFSRKLEG
                     SMIDPVAFGMLTSGGRDEFINTESIGNMSFLSAYLSFYNENNELLAYLNIPYFAMQNL
                     LTKETSNLIVTLVNFALLFLLIMMWVAVFLSERIIRPLSMLQEAMASVAYGRKNEHIR
                     YDSRDEVGELVRQYNRMTDELEESAGKLARTEREMAWREMARQIAHEIKNPLTPMKLN
                     VQQLLKSWNDRVSDFEKKIESFTVNQIEYIDNLSNIASAFSSFAKMPVAEPAEVDVLA
                     QLNTTLAMFGHADDTTITLESGNITRAVVMADREHLNSIFSNLLKNAIQSIPPGRMGE
                     IRIALSADDSHVRVRFIDNGTGIPDELIPKMFTPNFTTKSSGMG"
     gene            complement(3576..4049)
                     /locus_tag="PLA17_02295"
     CDS             complement(3576..4049)
                     /locus_tag="PLA17_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPK84274.1"
                     /translation="MKMVLLLTGKTDQSWIRDGVAEYEKRISKYARLETITLPDIRNP
                     GNRPAEWVKAAEAEKILAQFRSDDHVVLLDERGKGYSTLELADWMRRTVMLPAKRIVF
                     VVGGPYGFDSSVTARADHMLSLSRLTFSHQVVRLLFAEQLYRVLSVIAGDPYHHE"
     gene            4200..4601
                     /locus_tag="PLA17_02300"
     CDS             4200..4601
                     /locus_tag="PLA17_02300"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HPK84275.1"
                     /translation="MNKKIMAIAGVLLTFTFLLSAQDQPRIPAGIAIAFRTGSASGLS
                     EYLNKTVELVLPGKEDFYSKSVAETILKDFFTRYRSREFVIRHQGARGDAQYAIANLE
                     TDKGKFRVYFLLKKVDGNPLIHQIRIEADNE"
     gene            4594..5436
                     /gene="nadC"
                     /locus_tag="PLA17_02305"
     CDS             4594..5436
                     /gene="nadC"
                     /locus_tag="PLA17_02305"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPK84276.1"
                     /translation="MSDNQLRSFIINALAEDTGDGDHSSLASIPDTATGRARLIVKES
                     GIIAGLRVAREVFSIADPSLQVEILLHDGDRVQYGDIALTVSGKAHSILRAERLVLNF
                     MQRMSGIATATALYTEKVSDTNAKITDTRKTTPGMRALEKEAVRTGGGVNHRMGLYDM
                     IMLKDNHIDYAGGIAAAIRRTQEYLSANRLNLKIEVEARNIREVAEILETGGVDRIML
                     DNFNIPETREAVALIAGRTETESSGGITLENVRDYALCGVDYISIGALTHHIKSLDMS
                     LKAY"
     gene            5446..6402
                     /locus_tag="PLA17_02310"
     CDS             5446..6402
                     /locus_tag="PLA17_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPK84277.1"
                     /translation="MSARKTLIAITNRIRKAVLPGFDNAPLELVAKLIVKGLFGRGVL
                     VTRASSIAFNMLMALLPATIFLFTLIPFVPIPNFQAELVKLFENFLPVTAYNLFETTI
                     IDVITNKSGTLLIVMFIATIIFSTNGIHALMHAFNVSVHDFASRSWIQQRKIAIFLLV
                     FILIMFSAAGALIILSRVVVNRLVEIGVLEMNLVFYIVMAFKWLLIIVMLFFAISTLY
                     YLVPAHRKDFRYISPGSILATSLFIITSLAFSAYVNNFGQYNKLYGSIGTLIVILIWL
                     YLNSVALLVGFELNVSIRAATCNPENGCRQEPDHNPPSEVNG"
     gene            complement(6500..8374)
                     /locus_tag="PLA17_02315"
     CDS             complement(6500..8374)
                     /locus_tag="PLA17_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HPK84278.1"
                     /translation="MKRFIYTLIIAFVLPSAAFAYKLSVDSIPLELRENAAAVVRTHQ
                     MVYTVLSPGKAKASHRLVLTLLNESVDHLRLYEINYDKFRSVTRINAAVYDANGKFIE
                     AIPSSRIMDVGEGGGFISDARMKRIIFPVNRYPFTIEVEYDVTINGSLNLESWSFHPN
                     PYISVEESSVQYIIPSLVPFRYSGHYMNASFDSIRTADNTIYTWVQRKMPAIGKWFFS
                     PLIVSRTPFLLASIEDFEFGGIKGSMRTWKSLGQWAWELNKGLDQLGEAEKKRVAELT
                     SGLKSDREKAEALYRYMQSRTRYASIQLGIGGYKPAPAMEVSEKGYGDCKGLTNYMYA
                     LLREAGITSHYTWVRAGTNKSIIPAFVSDQFDHIILCVPLQGDTVWLECTSQTQPFNF
                     LGSFTCDRDVLLITPDGGKMVRTPPFSHSYRKSTGIINVNRREASKGSLRMTSGGLGF
                     DKNQIFSGKSVIEMKRMINEGMALGTFRADTAAYREITEGEPVSELDFDLTLNDFAVV
                     AGTRIHFNPCLNGFGHQPFDTVSVRIYEVEELIDSIVYRFPAEYRAEHIPSPVSAEGP
                     YGSYRYSIETLDDGSLLFRRKLSLNKGIYTGDRARELFVFLNTAARSDQRRIYLNRAQ
                     "
     gene            complement(8386..10344)
                     /locus_tag="PLA17_02320"
     CDS             complement(8386..10344)
                     /locus_tag="PLA17_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPK84279.1"
                     /translation="MNLPKLLAVPLLLLIANPETVMSQIRDITFGDIPREDLEMSEYN
                     PDPSADAVILENYASTFMRSGERIQVITDCHVRIKIINTDGLDYANVELPYWSGEKII
                     ALRAASYNLEEGQVVQNMVDKKSFYRDNASRYRNILRFSVPNVRPGTVIEYKYTLESP
                     DYFSLYTFKFQEEIPVRRCVFRVEFPGYFEYKFVTGGDFKIINYKAEQKRVVFGRSTI
                     EGFSGTWRGSNIPAYRKEPFSTGSDDYFAHIGFELSKINIPGYYFQDVSPTYQKFSEK
                     LLDRDDFGGYINKSSVVNEKVEELKAQGGSGTDLLRRIYAFVSDYMMWNGYNDFTASA
                     SMARVYREARGNSADINLMLLAMLRKAGITSDPVILSTRENGLINPYFAINSKFNHVV
                     IFAVADGKSWIIDATDPIRPFNMLPTECLNGQGWVVNRHGGSWADINNGEHYGEDVRL
                     EMNLDENGSLTGNATTVYESYDAWLIRKLCYLQGEDAYRNFVQASKPKWRISGLGLEN
                     LDDSEKPIIEKTTMTIPDAAERSSIFMYLNPVVDGRTESNEFHAGERLSVIDFTCPDF
                     WKYSCSIKIPDGWAVVNLPQSVNLRLDGGGAVFTYNISAEDGTIRLDSEISLSTVTFV
                     PERYETIRNFWSSIIRKQAEVVILKKEI"
     gene            complement(10364..10552)
                     /locus_tag="PLA17_02325"
     CDS             complement(10364..10552)
                     /locus_tag="PLA17_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84280.1"
                     /translation="MIRSLTAEPHWKERYTWEGIFLSLLNGMEVFILEEVIEPERTLV
                     ICGSITLPWKINRKNERD"
     gene            complement(10573..11097)
                     /locus_tag="PLA17_02330"
     CDS             complement(10573..11097)
                     /locus_tag="PLA17_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84281.1"
                     /translation="MIRDILKYTLLALALLFAGCEDERVPRDYPRVRTLEVTGITPDG
                     ALFAGEVYEPGTGKISDHGFVCGISTPGIDSDNKVYLDSFEGPGRFAKEIPTTLTEGV
                     TYKVTAFVKSGEYTVYGNVIRTGMPNFRFTLGLICGRTHEFVYQTGGYDIESPYSLTV
                     TGSQWESLRRLRIR"
     gene            complement(11107..13842)
                     /locus_tag="PLA17_02335"
     CDS             complement(11107..13842)
                     /locus_tag="PLA17_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPK84282.1"
                     /translation="MIRDILKYTLLALALLFAGCEDERVPRDYPRVRTLEVTNITGEG
                     ALFSAEVYDAGNVEITGHGFTWSREKPEINLNDRVNLGPFSGIGHYSSRITTALEPGV
                     KYKVAAFIRAGNYTVYGNTVEFESLGSLGPVITGITPERALCGDTILIRGKNFSWVGH
                     NNKVFFNETAAKVCNEVTDTTLLAVVPFSLTVPEQTVSVEVTGNRTVYTGSKLIVDLP
                     QIESIIPSQARWGDTITMSFRYLKKSDNIRFFIDQTQLVLTEAYDGETARAIVPFEAV
                     QPRIDLSVSVSGTRLVFFGSFTLLPPIITSIYPVMASWSDTVTLYGVFNANQAATQVL
                     FDERAANIVSVARDSIKVIVPNDLLGTTAVLTYKYGDFTCSSTQMFSLLPPVIETVSP
                     MSDYTGGIVTITGKYFKNYYTTVKFNDILSSVISVTGTSIQCYAPGNFCGPSEISVTV
                     CGQTTLFGQTFDLTNPSVVSFYPTHASPGDTITIEGLNLQNVSLFLISIKPDNPAYGG
                     YGFETVTKEQGLATAIVPSADYTSGLVTAWAWRDWIESYLAGDEMFSIDAPVINSFSP
                     SSGSAGTVVTLSGEHFSLVPQYNRVTINDYPVEIVSCSRNEIRFRMPNMSAGNYHISL
                     SICGHQVSSAGTFENISPWRRLQDLPFTNNSFTMDFGDEVFVAAPVASSNVTLYKFVP
                     ENYSFVTAGEVHPSLSYFGRTVVKGDRAYMMANTYSEVKFLEFNRNTMSLSVVSDPPG
                     KVSTHTILMDGDSVIYAGGGDSLTYRGYYVSEFWKYNLFSGTWKRLKDLPFFCMTSSE
                     FTINGRNYVISIDKKLWEYNPVADTWSHISTYPGTGTYDMMIVVCNGRVYIGHGRYGN
                     NQIYYYDPQTNSWDELLNEFPRFRVKPVDFEYGGKIYFGGSDRNDFWEYDPMLDH"
     gene            complement(13852..16701)
                     /locus_tag="PLA17_02340"
     CDS             complement(13852..16701)
                     /locus_tag="PLA17_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPK84283.1"
                     /translation="MHTFRSSLLSTVIACMSALTLFLSGCEDERVPREYPRVRTLEVT
                     GITEEGALFSAEIYDAGNVEITEHGFTWSFTEPSVSSDERIYLGGFSGTGRFEAEIST
                     ALAEGVTYEVCAFVKAGEYTVYGEKIKFISLGSKAPVVTGFSPLSAGWGDTITVTGEK
                     FSHRNITNRIYIDEKACNPFYSSDTLLRFVLPIEVEKTSNSLSVSIMGNIATANEKLL
                     LITPEFYDYSPYEVLWGDTVTFTGHHLGLIGYYPGDGMLLNGLIRSGVACRSPGEVSF
                     VIPGQLEEVSSTVSINYGPFSYTFPRSLTLLPPVADSIRPAEGTWGTTVILYGRFNMV
                     MERNKIMFGDKLARIISVACDSAIVKVPDDLLEYVSKVSYQSAPFPASEFPGSFYLKR
                     PEITGFSPAEGYVGERVTIRGRYFSKNAATVHIGGGQAIVRSANDSVIVCYVPGDAYG
                     ECDVTVTQMDYSVTAPGKFNSTNHVITGIDPPLPAFGETVTVRGTNFRPGTLIYIGPY
                     QIMTLSQTENEIKFVVPEWLPYQAGSLIAKYINPNSGYNPESSFTYPGQFPVKDFTVT
                     GVTPVSGEAGDILTISGSDFGTPYITFGSVQGEVLESTSSSITVRVPPLSAGEHTINV
                     TIGDRTHACPVKYTHSSAWMQLADLPFLYDYGCTFDLGEEAYVITGGKTDTYDKEVYR
                     FETSTKGFTRMAGTFRSGILNPISCTLGGKGYMIGQKSTSWTGIGFELFDPDNMTVSK
                     LPDYPGSLNTNPCIIADDSVIYAGCGKIAIPNYFTWYKDFWKYSPATGKWTRLADCPY
                     NVSFSNQVYIDGRLIFLGNSHDTGGTRYLLEYQPLTDTWKETVMDENEFDYWLLLGSR
                     LGARVSVVNGGKWYVGFGDLYQTHEGYGYTNPDINNRFYVFDPVDDSWGTISNVVAPP
                     RTFALAFSTGGKIYIGGHQIYKWYDFWEYDPMLDH"
     gene            16853..18085
                     /locus_tag="PLA17_02345"
     CDS             16853..18085
                     /locus_tag="PLA17_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84284.1"
                     /translation="MKRYLLTAVMTIFFTGFVSSQKEFLNGYIIKNDGSYTYGQVANI
                     SKGYSVEECLFRWFDISAVYSFKPEEIRAFGFTYGMRYKSVMSGKRGFFMACLVDGKV
                     DLLYDGKKMYLDGEGIDLIPLDNGSGSTILDGKRVSFKGYRDLLEKLPDPGDNFTVPS
                     DLPLSPEKMAEVIAAYNKSQGTDATMLAMKNPGGVHEEMRNLGLLVSNYGFLAGMNAS
                     RYDARKVVAARNLFVPEMDFFEVIPVAGAFFNRPLSRNRDFLSLNLEVMVFSTNIYMY
                     SETVQSSVTTRSDITLSYTGIKIPLSLRLTFLEGSYKPFINAGLFAMVNHGGKYTRDG
                     EVENSMHVVRPFTDNSLTVSKTINGFLGGIGMKKEFNLKQSISLEFRAEYGSGIYAQD
                     GLKQNTLSFNVIAAIDFF"
     gene            18263..19561
                     /locus_tag="PLA17_02350"
     CDS             18263..19561
                     /locus_tag="PLA17_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84285.1"
                     /translation="MKKAVFISALMILAACQHKENTFWFNADMAKKYFSAVEEICNSD
                     NGKLWGENLFGPVMYVDGQSRTLYANFPDKEGLLRERDGVYTGVLPKERIIANNVIEF
                     GGVKYAMVPLPEKDDRYRMVTRTIHSLFHCYQDRHGLKPSTFSTRHLNEKNARLYLKL
                     EWKALTNAITSSGEKRDQAIRDALVFRGARRELFPSAITDENKFENFEGLTTYTYVKL
                     CTSGPEEIRTRILEYLDRVYQNRSYAFGYGFVNGALYAVLLNDKDFDFKQIQTQDYDL
                     GKAVLDAYGVTLPAVCRDVAGSLAFNYDIQAIRAEESQRESKINEYTRRIVATFYEKP
                     VLTISMESPNFSFEPEDIDYLDTLGTMYKKLRVSDNWGRLAVDKGSALITNDLRTLRV
                     SAKNLLVERNHISGEGWHLVLNDGWRPEQLDNGNYTITRH"
     gene            complement(19571..>20206)
                     /locus_tag="PLA17_02355"
     CDS             complement(19571..>20206)
                     /locus_tag="PLA17_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPK84286.1"
                     /translation="KPIDGVTHRETLEMFGDLIKILHPFMPFITEEIWQRLADRPEGS
                     SIMVEDMPLPAPYEAGMLTRFDTVREIVSAVRTVRKEKNLPLREPLVLCASPGATGNE
                     FDPVLIRMCNLSEIRVTEEKVEGAASFRIGTSEFFVPLSKGIDNEDEIRKLEDELVYQ
                     EGFLASVLKKLSNEKFISKAPQKVLDNERKKEADSRARIATIKESLAHLKK"
BASE COUNT         5288 a         5096 c         4653 g         5169 t
ORIGIN      
        1 gccccatacc tgatgatttt gtggtaaaat taggagtgaa cattttggga atcagctcat
       61 ccggaatacc ggtgccgttg tcaatgaatc ttaccctcac atgactgtca tcagcagaaa
      121 gtgctatcct gatttctccc attctgccgg gaggtataga ctgtatcgcg ttcttcagca
      181 ggtttgaaaa gatactgttc agatgttccc tgtcagccat cactactgcc ctggtgatgt
      241 tgccactttc cagtgtaatg gttgtatcgt cagcatggcc gaacatggcg agagtggtgt
      301 tcagctgggc cagaacgtca acctcggcag gttctgccac cggcatcttg gcgaaagatg
      361 agaaggctga agcaatattg gaaaggttgt caatatattc aatctgattg actgtaaagc
      421 tctcgatttt cttttcaaaa tcagaaaccc tgtcattcca ggattttaac agttgctgca
      481 cattgagctt catcggggtg agagggttct tgatctcatg ggcaatctgc ctggccatct
      541 cacgccaggc catctcacgt tcggtgcgcg caagcttgcc ggcgctctct tccagctcat
      601 cggtcatccg gttgtattgt ctcacaagct cgccaacctc atcgcgactg tcatatctta
      661 tatgttcatt cttccttccg taggcaactg aagccattgc ctcctgaagc atactgagcg
      721 gcctgataat cctttcactc aaaaagactg ccacccacat cataatcaac aggaacagaa
      781 gtgcaaagtt gacgagtgtg acaataaggt tcgaagtctc ctttgtcagg aggttctgca
      841 tggcaaaata tggtatgttg agataggcca ggagctcgtt gttctcattg taaaatgaaa
      901 gataggctga gaggaaactc atatttccaa tgctctcagt attaataaat tcatccctgc
      961 ctccggatgt aagcataccg aaagcgacag gatcaatcat gcttccttca agcttgcgcg
     1021 aaaagacctc aggccttgat gtggctatga gcctcccgga aggtgaatag agattaatgt
     1081 cagtaaagaa gacatttgag aacttcacca gcagatggtt aagagtggga taatcaggcg
     1141 tttgccaccc tctgtcaagc gtcttctccg ttgagagctt gtgatccagc tcaatggcga
     1201 gcgatgccgt tttttcactt attatcctta ggtgattact gcggaactgt ctggtgctca
     1261 atataagtgc tcctgtaatc acaattatga agaccatggt aaggacggct gcaaatgaca
     1321 gttgcagacg ccggcgaaaa gtatccgtct tcagtagctc ggcaggcttc atagtgaaga
     1381 tgacaagcag tattattacg taggtaaggt tggcaatgaa gagataggca aaagtgatta
     1441 tccggtcaag gaactttacc ctggttacag tgacagcaag ggtcatgtca cccagtgtat
     1501 agtacaggtg tttgtaatcc ctgccatcct caaacctgaa ctcctccctc tccggctgac
     1561 ccccggggat ggaggcatca tacggatatt cgcctgacct cagaaccagc acgcctttgt
     1621 tgtatttcgc gtacgagata tccttaagca tgggaaatct ctgataacca ccgtcgagca
     1681 gcaactcagg gtagcctgcc aggaacgtct ctatatggct gaccagctca atgaagagcc
     1741 cgttggtcac tcccggtgat atctcataca ggagctttga caagtaccag gctcttccgg
     1801 cctggttgcg catgaagtaa aagccggtat tcgtgatggt atcaccctca ttcattatgc
     1861 ggttgtcaaa ccagtcgaag cagttggcca cctctgcacc cgtggagccc aggctgaggg
     1921 gtgagtcatt gcggcagatc acgatgctga tatcatagtt ctgccagtaa ccgtagaaat
     1981 agccatcctc aagatacctg tagacagaat tgaggtcgcc gggagtgatg aattcctttt
     2041 tgaccagccg ggcaagcacg gtgtcactct ccagggcagg ccacatgtca ataatcatcc
     2101 cttctgccac catatcattg tcactggcca gtgagacggc cattaccttc atgtttctct
     2161 cctccctgat gattgagtat ttgattataa gtgcggtgga gagcatggcc gttattaccg
     2221 agtagatgat tgcgagtgat accccggaat aggatttcct ctgccatata aaagctgtgg
     2281 tgatcatcac cagtatccag aggatgctcc agccattgca gccggtatcg gcgaggcatg
     2341 ccagagggag cagcaaggtg atgccgccgg caactgcaag aatctcacgc gacgaccatc
     2401 cgtgcatcat cctggctgcg tacatgataa gtatcacagg gatggtcagc aacagcatca
     2461 cagagactat gccggccagg ctcatgaagg tcatgtcaag tatccggaat gcctcaaaac
     2521 tgattgccga attcaccacc aggtccctga aatgggcctc aaccaggagg aatatgacat
     2581 atcccattgc cagtatcact cccgggacca gatatttcca aatacccttc ccgctccacg
     2641 gcttgctgaa cctgtcgctc ctgaatgtga accggatacc tgaaaggacc agtatgccga
     2701 gaagcaggac gtgtcctaca gtcggaatca acctgccggc tgaccagttg aacggtgaaa
     2761 acagcccggt tgaggttacc gaacgcggca atcccgtcag aagaatgatt ccgtataccg
     2821 ccagaagcgc agcaaatgca aagacaatac ctgatggcgt cctggatttc atgtcaatat
     2881 gccgggcaac aaggagtacc agccttacca ggaggaaaag gacaataagc cataacagcg
     2941 ccgggatggc caggaatatg gtgttgggct ttgtttcggg gaaaataaga ccgaagtgat
     3001 attcttcctc cgatccccat ataatgaagt ccgaggcgga agggtcaaag gtgattttga
     3061 ccccatcagg aagagaaaac gtctcgggaa agcctgtctg aaggatatca ttcacgatgg
     3121 ggtactgcct gtacaccttg atcagcgcca ccatctcata ttcactgttt tcaaggtata
     3181 ccggtatgaa ccagccattg ctgtagaaga ccggcttatg ctcatcaaaa tgttcctcat
     3241 agatcaccgg gaaggcaatg ctgttgtctg accagtaggc tatgcgtcgc tcatggtaaa
     3301 ccagcagggt gatccccttc ttcagggcgt cgctgcccag agtactatgg aacagtaccg
     3361 aagaatcacc cgcttcggag atcatttctt cagctgtcct gagcaactcc ccggcataat
     3421 tctcaagtga tttgagcctg gcatcagcgc gtgctgtacg gtatctccac tccaggtcac
     3481 tgaaatagag aaggtcagca actgctgcca gggcaacagt gatgagaagg gcaactccta
     3541 ctatcctgat gttcttcagt ttctcagcca taaggctatt catgatgata tgggtctccg
     3601 gcaattaccg aaagcaccct gtatagttgt tccgcgaaca ataatcgtac cacctgatgt
     3661 gagaaggtga gcctcgagag tgacagcata tgatctgccc ttgccgttac cgaggaatcg
     3721 aagccatagg gtcctcccac cacaaacacg attctttttg ccggcagcat cactgtccgg
     3781 cgcatccagt cagcaagttc cagcgttgaa taacccttcc cgcgttcatc aagcaggacc
     3841 acgtgatcat cactccggaa ctgtgcaagt atcttctctg cctccgcggc ctttacccat
     3901 tctgccgggc ggttgcccgg gtttctgata tcggggagag tgattgtttc gagacgggcg
     3961 tactttgaga ttctcttctc atattccgcc accccgtctc ttatccatga ctgatcagtt
     4021 tttccagtca gcaggagcac catcttcatt actctccccg gtttctgttg agtatggcaa
     4081 atatagcaat taagcccttt attgcgggca gggcttcctg ataattaaaa gtggttcggt
     4141 ttttgatata tttggttaaa tttgctttgt ccttacttgt aaagcataaa ggaaacaaaa
     4201 tgaataaaaa gattatggca atagcaggtg tgcttctcac cttcacattt ctgctttctg
     4261 cccaggatca gcccaggata cctgcaggta tcgcaatcgc cttccgtacg ggaagcgctt
     4321 cggggctctc cgagtacctc aacaagacag ttgagctggt tctgccgggc aaggaggact
     4381 tttacagtaa gagtgtagct gaaaccatac tgaaagattt tttcacacgc taccgctcac
     4441 gggaatttgt catcaggcac cagggggcga ggggcgacgc ccagtatgca atagcgaacc
     4501 tggaaacaga taagggcaag ttccgcgttt atttcctgct gaagaaagtt gacggtaacc
     4561 ccctgataca ccagattagg attgaagctg acaatgagtg ataaccagct acggtcattt
     4621 attatcaatg cccttgccga agacacgggc gacggagatc acagttccct tgcatccatc
     4681 cctgacactg ccacgggccg cgcaaggctt atagtaaagg aaagcggcat catagcaggg
     4741 cttcgcgttg cacgcgaagt gttcagcatt gcagatccgt cactgcaggt ggagatactt
     4801 cttcatgacg gcgacagggt gcaatacggc gacatagctc tgacagtgtc aggcaaggct
     4861 cactctatcc tgcgcgccga gagactggtg cttaatttta tgcagagaat gagtggcata
     4921 gccacagcca ccgccctcta tacggaaaaa gtcagtgaca caaatgcaaa aatcactgac
     4981 acccgcaaaa ccaccccggg aatgcgcgca ctcgaaaagg aggcagtgcg caccggagga
     5041 ggagtcaacc accgcatggg cctctatgat atgatcatgc tgaaggataa ccatattgac
     5101 tatgccgggg gtattgcagc agcaatccgg cggacacagg agtacctctc agcaaacaga
     5161 ctcaacctga agatagaagt ggaagcacgg aacatcaggg aggttgcgga aattcttgaa
     5221 acgggagggg ttgaccggat tatgcttgac aatttcaata ttcctgaaac cagggaggct
     5281 gtggctctga ttgccggcag aactgagacc gaatcatcag gaggcatcac cctggaaaac
     5341 gtgagagatt atgcgctgtg tggcgtggat tacatttcaa taggggctct gacccaccat
     5401 ataaaaagcc ttgacatgag cctgaaagcc tattgataac cctgtatgag cgccaggaaa
     5461 acgctgatcg caataaccaa cagaataagg aaagccgttc tgcccggctt tgacaacgca
     5521 ccgcttgagc tggtggcaaa gcttattgtc aaggggctgt tcggacgcgg agtgctcgta
     5581 accagggcct catccatcgc cttcaacatg cttatggcac tgctccccgc aacaatcttc
     5641 ctcttcacgc tcataccgtt tgttcccatt ccaaattttc aggctgaact tgtaaagctg
     5701 tttgagaact ttctccctgt gacagcatac aaccttttcg aaacgaccat tattgatgtc
     5761 atcacaaaca aaagcggcac cctgcttata gtgatgttca ttgcaaccat cattttttca
     5821 accaatggca ttcacgcgct gatgcacgcc ttcaacgttt cggtgcatga ctttgccagc
     5881 cggtcatgga tacaacagag gaagattgca atcttcctgc tcgtttttat ccttatcatg
     5941 ttctctgctg caggcgccct gataatcctc agccgcgtgg tggtcaacag gcttgttgaa
     6001 ataggggtac tggagatgaa cctggtattc tatattgtaa tggccttcaa atggcttctt
     6061 atcattgtga tgctcttttt tgcaatttcc actctctatt atcttgtccc tgcccacagg
     6121 aaggacttcc ggtatatctc cccgggatca atcctcgcca ccagcctgtt tatcattacc
     6181 tcactggcct tctcagccta tgtcaacaac ttcggccagt acaacaagct ttacgggtct
     6241 atcggaacgc tgatagttat ccttatctgg ctttacctga attcagttgc tctgcttgta
     6301 ggcttcgagc tgaatgtcag tatcagggct gctacatgca acccggagaa cggctgccgg
     6361 caggaacctg atcataaccc gccttccgaa gtcaatggct gagtgtccgc aggtcactga
     6421 aaaaaggatt tccggccgta ggccgaagtt cacagctgaa ctgaccggta aacgcaatcc
     6481 ctgatagctc tgagccctgt cactgcgccc tgttcaggta tatccttctc tgatcactcc
     6541 gggctgcagt gttaaggaag acaaacagtt cccttgccct gtcaccggta taaataccct
     6601 tgttcaggct cagcttcctc ctgaaaagga gagatccatc gtccagggtc tcaatactgt
     6661 atctgtatga tccataagga ccctctgcag atacaggcga cggaatatgt tctgccctgt
     6721 attcagccgg gaatctgtag acaatggaat ctataagctc ctccacttca taaatcctga
     6781 ccgaaacggt atcaaagggc tggtgcccga agccattgag acagggattg aagtgtatcc
     6841 ttgtcccggc taccactgca aaatcattga gagtgagatc aaagtcaagc tccgagacag
     6901 gctccccttc tgttatctca cggtatgcag cagtatcagc cctgaatgtg cccagagcca
     6961 ttccctcgtt tatcatccgc ttcatctcaa tgacactctt gccgctgaaa atctggttct
     7021 tgtcaaaccc gagcccgccg gaggtcattc tcagtgatcc cttcgatgcc tcccttctgt
     7081 tcacattgat tataccggta ctcttcctgt aactgtggct gaacggtggc gtacgcacca
     7141 tcttcccccc atcgggggtg ataaggagca catccctgtc acatgtaaag ctgccgagga
     7201 aattgaaagg ttgtgtctgg cttgtgcatt ccagccatac tgtgtctccc tgtaacggga
     7261 cacaaaggat gatgtggtca aactggtctg acacaaaggc aggaatgatg gatttgtttg
     7321 ttccggctct gacccaggtg taatgtgatg ttattcctgc ctccctgagc agtgcgtaca
     7381 tatagttggt cagccccttg cagtcgccat atcccttttc cgatacttcc atggcaggcg
     7441 caggcttata accgccaatt cccagctgaa tggatgcata acgggttcgt gattgcatat
     7501 agcggtaaag tgcttctgcc ttttccctgt cactcttcag gccggaggtc agttctgcaa
     7561 cgcgtttctt ttctgcctcg cccagctggt caagcccttt gttgagctcc caggcccact
     7621 ggccaaggct cttccatgta cgcatgcttc cttttattcc tccgaactca aagtcctcta
     7681 ttgatgccag gagaaatggc gtacgtgaaa caataagcgg tgaaaaaaac catttcccga
     7741 tggcgggcat cttcctctga acccatgtgt agatagtatt gtctgcagtc ctgatactgt
     7801 caaatgacgc attcatgtag tgcccggagt accggaacgg aaccaggctt gggatgatgt
     7861 actgtacgga tgattcttcc accgagatat acggattggg atgaaaggac cacgattcaa
     7921 ggttaagaga gccattgatt gtcacatcat actccacctc tatggtaaac ggatacctgt
     7981 tcacggggaa gatgatcctt ttcatccttg catcggagat aaaacctccc ccttctccta
     8041 catccataat cctcgatgag gggatagcct caatgaattt tccgttggcg tcatagaccg
     8101 ctgcattgat ccttgtcact gacctgaatt tgtcatagtt tatctcgtac aggcgcaggt
     8161 ggtcaacgct ctcgttcaac agcgtgagga ccagcctgtg gcttgccttt gcctttcccg
     8221 gtgacaaaac cgtgtaaacc atctggtggg tcctcactac ggcagccgca ttctccctta
     8281 gctcaagcgg aattgaatcg actgacagtt tgtatgcgaa tgctgccgaa ggcaacacaa
     8341 aagctataat cagggtgtaa ataaatcttt tcatgattga ccggatcaaa tttctttttt
     8401 cagtatgaca acctctgcct gtttgcggat aatggacgac cagaagttcc ttatagtttc
     8461 atatctctcc gggacgaagg tgaccgttga gaggctgatt tcagaatcaa gcctgattgt
     8521 accgtcttcg gcggagatgt tgtaggtgaa cacggctcca ccaccatcaa ggcgcaggtt
     8581 gaccgattgc ggcagattga caacggccca tccatcgggt attttgatgg aacagctgta
     8641 cttccagaaa tcaggacagg taaagtcaat aaccgacagg cgctccccgg catggaattc
     8701 attcgattct gtccggccat cgacaacagg attcaggtac atgaatatgc tgcttctctc
     8761 ggctgcgtca gggattgtca tggtagtttt ttcaataatt ggcttttcgg aatcatcaag
     8821 attttcaagt ccgagcccgg aaatcctcca ctttggtttt gaggcctgaa caaaattccg
     8881 gtaggcatct tctccctgca ggtagcataa cttcctgatc agccaggcat catagctctc
     8941 ataaacagtt gtcgcattgc ctgtcaggct gccgttctca tcgagattca tctccagcct
     9001 gacatcttcg ccataatgct caccgttgtt gatatctgcc catgatcccc catgcctgtt
     9061 taccacccat ccctggccgt tcaggcactc tgtcggcaac atgttgaaag gtcttatcgg
     9121 atcagtggcg tcaatgatcc agcttttgcc gtcagcgaca gcaaagataa ccacgtgatt
     9181 gaatttgctg tttatggcaa agtatggatt aattagtcca ttctccctgg tgctgaggat
     9241 aaccggatcc gatgtaatgc ctgctttcct taacatggcc agcagcatca ggttaatatc
     9301 agcggaattg cccctggcct ctctgtaaac cctggccata gatgctgatg ccgtgaagtc
     9361 attgtatccg ttccacatca tgtagtcaga aacgaaggca tatatccgcc tcagcaggtc
     9421 tgtcccgctg ccaccctgcg ccttaagctc ctccactttc tcattcacaa cagagctttt
     9481 attaatgtaa cctccgaaat catccctgtc aaggagtttc tcggagaatt tctggtaagt
     9541 gggtgataca tcctggaaat agtagccggg aatattgatc tttgaaagct caaacccgat
     9601 gtgtgcaaag tagtcatcag aacccgtact gaaaggttct ttacggtatg cgggtatatt
     9661 gctgccccgc cacgtgccgg agaacccttc gattgtactt cttccgaaca ccactctttt
     9721 ctgttcagcc ttgtagttaa tgattttaaa atcaccgcct gtaacgaact tgtattcgaa
     9781 gtatcccgga aattcaaccc ggaaaacgca cctcctgact ggtatctcct cctggaactt
     9841 aaaggtatag agtgaaaaat agtcagggct ctcgagagtg tacttgtact caatcaccgt
     9901 gcccggcctt acgtttggaa ccgaaaagcg gagaatgttc cggtaacggc ttgcattatc
     9961 acggtaaaag ctcttcttgt caaccatgtt ctgcacaacc tgcccctcct ccaggttata
    10021 ggaagctgcc ctcagcgcaa ttatcttttc accgctccag tagggcagtt caacattggc
    10081 ataatcaagc ccgtcagtgt ttatgatctt tatcctgaca tgacagtcag tgatcacttg
    10141 aatcctttcg cctgaccgca tgaatgtact tgcatagttt tccaggatca cggcatcggc
    10201 tgaggggtca gggttatatt cagacatctc caggtcttcc ctcggtatat ctccgaaggt
    10261 gatgtccctg atctgtgaca tgacagtttc aggatttgca atcaggagaa gcaatggcac
    10321 tgccagcaat tttggtaaat tcataatttt ctttttatga caatcaatca cgttcatttt
    10381 tcctgtttat tttccatggc agggttatac ttccacatat caccaaggtt ctctccggtt
    10441 ctataacctc ctccaggata aatacttcca ttccattcaa aagcgaaagg aaaatacctt
    10501 cccatgtgta tctctccttc cagtgaggtt cagctgtcag tgaccggatc atagcatatt
    10561 atctggttgc agtcatcgga tccgtaaccg acgcaggctt tcccattgtg accccgttac
    10621 tgtaagggaa taaggtgact ctatgtcata acctccggtt tgataaacaa attcatgggt
    10681 ccttccgcag atcagtccaa gggtaaacct aaagttgggc atacctgtcc tgatgacgtt
    10741 gccatataca gtatactcac ctgacttcac gaaggcagta accttatatg tgacaccttc
    10801 agtcagggtc gttgggatct ctttcgcgaa ccggcccgga ccctcaaatg agtcaaggta
    10861 aaccttgttg tcagaatcga taccaggggt gcttataccg catacaaaac catgatcaga
    10921 gattttgccg gtgccaggtt catatacttc cccggcaaac aaagcaccat caggcgtgat
    10981 gcctgtaacc tcgagggtcc tgacgcgcgg gtaatcacgc ggcacacgct catcttcaca
    11041 gccggcaaaa agaagagcaa gggccaataa tgtatatttc agaatatctc tgatcatctc
    11101 atttctctaa tgatcaagca tgggatcata ttcccagaag tcattcctat ccgagcctcc
    11161 gaagtaaatc ttgcctccat actcaaaatc aaccggtttt acccggaatc ggggaaactc
    11221 attcaacagt tcatcccagc tgttggtctg gggatcataa tagtatatct gattgtttcc
    11281 gtatcttccg tgaccaatat agaccctccc gttgcaaacc acaatcatca tgtcataagt
    11341 gccggtaccg ggatatgttg aaatatgtga ccatgtatca gctacaggat tatactccca
    11401 taactttttg tcaattgaaa tgacataatt ccgtccgttt atggtgaatt cactggaagt
    11461 catgcagaaa aacggaagat ccttcaatct tttccaggtt cctgaaaaca ggttatactt
    11521 ccagaactca ctcacgtaat aacctctgta ggtaagtgaa tctcctccac cggcatagat
    11581 taccgagtct ccgtccatca ggattgtatg cgtactgact ttgcctggag ggtcagaaac
    11641 aacactcagg gacattgtgt ttctgttgaa ctcaaggaat ttgacttctg aatatgtatt
    11701 cgccatcata tacgccctgt cgcccttgac aaccgtccgt ccaaaataac taagcgaagg
    11761 atgtacctca ccagcagtga caaaggaata attctccgga acaaacttat aaagagtcac
    11821 atttgagctg gctacaggtg cagcaacgaa cacctcatca ccaaaatcca tcgtgaatga
    11881 attattagta aaaggaaggt cctgaagcct gcgccaggga gaaatatttt caaaagtgcc
    11941 tgctgaactt acctgatgac cacatattga cagcgaaata tgatagttac ccgcagacat
    12001 gtttggcatc cgaaatctga tttcattccg gctgcaactg acaatctcaa ccggataatc
    12061 gtttattgtg accctgttat attgaggtac caggctgaaa tgttctcctg atagtgtaac
    12121 caccgtacct gcggatcccg aagatggcga aaatgaattt atcaccggag catcaatact
    12181 gaacatttca tcaccggcaa ggtaagactc tatccagtca cgccatgccc atgcagtgac
    12241 cagccctgac gtataatcag cggatggaac aatggctgtc gccagaccct gttcctttgt
    12301 aactgtctcg aaaccatacc ctccgtatgc cggattgtca ggctttattg agatcaggaa
    12361 cagacttaca ttctgcaggt tcaggccttc aattgttatt gtatcacccg gagaggcatg
    12421 agtgggataa aaagagacca cactgggatt tgtgaggtca aatgtctgac cgaatagtgt
    12481 tgtttgcccg cataccgtaa cgcttatctc tgaaggcccg cagaaattgc cgggggcata
    12541 acactgaatg cttgtacctg tcacactgat aacgcttgac aaaatatcgt tgaactttac
    12601 agttgtataa taatttttga agtatttacc tgtgatggtg actattcctc cggtataatc
    12661 actcatcggg ctgacagttt caataacggg aggtaacaaa gaaaacattt gggtagagct
    12721 gcaggtgaaa tcaccgtatt tatatgtcaa tactgccgtt gtacccaaaa ggtcattggg
    12781 tactataacc tttattgaat cccttgcaac agaaacaata tttgcagccc tttcatcaaa
    12841 cagaacctgg gttgcagcct gatttgcatt gaaaacccca taaagtgtaa cagtatcaga
    12901 ccacgatgcc ataacaggat aaatactggt gattattgga ggaagaagag tgaaactccc
    12961 gaaaaataca agcctggttc cactgactga aacagacaga tctatccggg gttgaacagc
    13021 ttcaaaaggt actattgccc tggctgtttc accatcgtac gcctccgtta aaaccagttg
    13081 ggtctggtca atgaaaaacc tgatgttatc tgatttcttt agatatcgga aactcattgt
    13141 aattgtatca ccccaccggg cctgggaagg tataattgac tcaatctgcg gaagatccac
    13201 aatgagtttg ctgccggtat aaacagtccg gttgccggta acctccacgc tgactgtctg
    13261 ttctggcact gtcagtgaaa agggcacaac cgcgagaagc gttgtatcgg taacctcatt
    13321 gcatactttt gcagctgttt cattgaagaa tactttgtta ttgtgaccaa cccagctgaa
    13381 gttttttccc ctgataagga tagtatcacc gcaaagtgcc ctttccgggg taatgccggt
    13441 tattaccgga cccaggctgc ccaggctctc gaattcaact gtgttcccgt aaactgtata
    13501 gttgccggcc ctgatgaagg cagccacctt gtattttacc cccggttcca gagcagttgt
    13561 tatcctggaa gaatagtgcc cgatgcctga aaatgggcca agatttaccc tgtcattcaa
    13621 gttgatctcc ggcttttcac gtgaccaggt aaatccgtgc ccggtgattt ctacattccc
    13681 ggcgtcataa acctctgcgg agaagagcgc accctccccg gtgatgtttg taacctcgag
    13741 ggtcctgacg cgcgggtaat cacgcggcac tcgctcatct tcacagccgg caaaaagaag
    13801 agcaagggcc aataatgtat atttcagaat atctctgatc atctcatttc tctaatgatc
    13861 aagcatggga tcatattccc agaagtcata ccatttataa atctgatggc ctccgatata
    13921 tattttgccc cctgttgaaa acgccagagc aaaagtcctt ggtggtgcta caacattgct
    13981 gatggttccc cagctgtcat caaccggatc aaaaacatag aaacggttat ttatatccgg
    14041 gttagtgtat ccataaccct catgcgtctg atacaagtca ccgaaaccga cataccattt
    14101 gccaccgttt accacactta cccttgcacc gagcctggat cccagcaaaa gccagtaatc
    14161 aaactcattt tcatccatga cggtctcctt ccatgtgtct gtcaacggct gatattcaag
    14221 caggtatctc gtgccaccag tatcatgtga attcccaagg aaaatcagtc tcccatcaat
    14281 gtaaacctgg tttgagaagg agacattata cggacagtca gccaggcgcg tccatttgcc
    14341 tgtcgcgggg ctgtatttcc agaagtcctt gtaccatgta aagtagttgg gaatggcgat
    14401 tttgccacaa ccggcataaa tcactgaatc atccgcaata atacaaggat ttgtattaag
    14461 gcttccggga tagtcaggca gtttgctgac cgtcatatta tcaggatcga acagctcaaa
    14521 cccgatcccg gtccagctgg tgctcttctg ccctatcata tagcctttcc ctccgagagt
    14581 gcaggagata ggattaagga taccggaccg gaaggttccg gccattcttg tgaatccttt
    14641 tgttgaagtc tcaaaacgat atacctcctt atcataggtg tctgtcttgc caccggtaat
    14701 aacataggct tcctcgccta aatcaaaagt acagccatag tcatacagga acggaaggtc
    14761 tgccagctgc atccaggcac tgctgtgcgt gtacttcacc ggacaggcat gtgttctgtc
    14821 gccgatagtg acattgatgg tatgttcgcc ggcagagagc ggtggcaccc tgactgttat
    14881 ggatgacgaa gttgactcaa gtacctctcc ctgcacggat ccgaaggtaa tatagggagt
    14941 gccgaaatca ctccctgaga ttgtaaggat atctcccgcc tctccggata caggcgtcac
    15001 tccggtcacc gtgaaatcct tcaccgggaa ctgtcccgga taagtaaagg aagactctgg
    15061 attgtaacca gaattaggat ttatgtattt ggcgatcaga ctccctgcct gataaggcaa
    15121 ccattctggt acaacgaatt ttatttcatt ttctgtctgc gataaagtca tgatctggta
    15181 aggccctatg tatataagcg ttcctggcct gaagttagtc ccccttacag tcacggtttc
    15241 gccaaaagcc ggcagcggcg gatcaatacc ggtgatgaca tggtttgtcg aattaaactt
    15301 ccccggagca gtgacagaat aatccatctg ggtgactgtc acatcacact cgccataagc
    15361 gtctcccggc acataacaga ctatgactga atcattggcg gatctgacaa tggcctgtcc
    15421 gcctcctata tgaacagtag cggcattctt actgaaatag cgtcccctga tggtgacacg
    15481 ttctcccaca tacccctctg ccggagagaa tccggttatt tcgggtcttt taagataaaa
    15541 actgcctgga aattcactgg cgggaaaggg cgctgactga taacttacct ttgagacgta
    15601 ctccaggagg tcatcaggaa ccttgacaat ggctgaatca catgccacgg aaataatccg
    15661 ggccagtttg tcaccaaaca tgattttgtt tcgttccatg accatgttga atctcccgta
    15721 aaggatcact gttgtgcccc aggtaccttc agccggtctt attgagtcag ccacaggagg
    15781 aaggagagta aggctccggg ggaaggtata actgaatggc ccatagttaa ttgacactgt
    15841 tgacgagact tcttcgagct ggcccgggat cacaaaagag acctctccag gactcctgca
    15901 tgcaacccca ctcctgataa ggccgttcag caacatgccg tccccaggat agtagccaat
    15961 caaccccagg tgatgacccg tgaaggtaac agtatcaccc cacagcacct catacgggga
    16021 gtaatcataa aattcaggag taatcagcaa aagcttttca tttgcagtgg cgatattccc
    16081 cataatgctg actgataaac tgttactggt tttttcaacc tcaatcggaa gtacaaacct
    16141 cagcagtgta tcgctggaat aaaatggatt acatgccttc tcgtcaatgt aaattctgtt
    16201 cgtaatgttc ctgtgactga acttctctcc ggtaactgtt atcgtatctc cccagccggc
    16261 ggagagcggg ctgaaacctg tcactaccgg tgccttgctt cccagactga tgaatttgat
    16321 tttttcacca tatacagtat actcaccagc tttgacaaaa gcacatacct cgtaggtaac
    16381 gccttcggcc agtgcagtac ttatctcagc ctcaaacctg cctgtgccac tgaaaccacc
    16441 gaggtatatc cgctcatcgg aactgacaga tggttcagta aatgaccagg taaatccgtg
    16501 ctcggtaatc tctacattac cggcgtcata aatttctgcg gagaagagcg caccctcctc
    16561 tgtgatgccc gtaacctcga gggtccttac ccggggatac tcacgcggta cacgctcatc
    16621 ttcgcaaccg ctgagaaaca atgtcagcgc tgacatacag gctatgactg tagatagaag
    16681 agagctcctg aaggtatgca tccctgatga cattagtctt taattaccag acattggtaa
    16741 atctactgaa aaattcaatt caacggagca taatttttaa acaaaattta gcatttacac
    16801 tcctgattac gatttatttt cttaagttag tactgttcat tcctgcaaga caatgaaaag
    16861 atatctgctg acagccgtga tgacaatttt cttcacaggt tttgtctcct cccagaaaga
    16921 atttctgaac ggctatatca ttaaaaatga tggcagctat acctatggcc aggtggccaa
    16981 catctcaaaa ggctattccg tggaagaatg cctcttccgg tggtttgaca tttccgccgt
    17041 atattctttc aagccggagg aaataagggc attcgggttt acctacggaa tgaggtacaa
    17101 gtcagtcatg tcaggtaaac gcgggttctt catggcatgc cttgttgacg gcaaggttga
    17161 cctgctatat gacgggaaaa aaatgtatct cgacggggaa gggattgacc tgatacccct
    17221 tgataacgga tcaggcagca ctattctgga tggaaagaga gtcagtttta aaggctacag
    17281 ggaccttctg gaaaagcttc ctgacccggg tgataatttc accgtgccct cagacctccc
    17341 tctctctccg gaaaaaatgg cagaggttat tgcagcatac aacaaaagcc agggtactga
    17401 tgccacaatg cttgcgatga aaaaccccgg aggcgttcat gaagagatgc gtaacctcgg
    17461 attactggtg agcaattacg gattccttgc cggaatgaat gcctcaagat atgatgcgcg
    17521 gaaagtggta gcagcaagga acttattcgt gcctgagatg gattttttcg aagtgattcc
    17581 ggtggcaggc gccttcttca accggcctct gagcagaaat agagacttcc tgtcactgaa
    17641 ccttgaagtg atggtcttca gtacaaatat ctacatgtac tccgagactg ttcagtcttc
    17701 tgtcacaaca agaagcgata tcactttgag ttataccggt ataaaaatac ctctgtcact
    17761 aaggcttaca ttcctggaag gaagctacaa acccttcatc aacgcaggtc tcttcgccat
    17821 ggtcaaccac ggcgggaaat acaccaggga tggggaagtg gaaaattcca tgcatgttgt
    17881 aaggccgttc actgacaatt cactgacggt tagcaaaacg ataaacgggt tcctcggagg
    17941 aataggaatg aagaaggaat tcaatcttaa acaaagcatc tcactggaat tcagggctga
    18001 atatggctcg ggaatctatg ctcaggatgg cctgaaacaa aacaccctca gctttaatgt
    18061 tatagctgca atagactttt tctagtgcag ccgcttcccc gccggtgaat atgcagcccg
    18121 gaaccggccc tgcccgcggt gatctccggt gcataactga tctccacccc gaaacaaaat
    18181 catgaataaa tccaggcaga aaaatgcccg cagcccgggt taatctattt ttattatttt
    18241 tgaacctgaa gtttccaaac ggatgaagaa agctgttttc atatctgccc tgatgattct
    18301 tgctgcatgc cagcataagg agaacacttt ctggttcaat gccgacatgg caaagaagta
    18361 tttcagcgcc gttgaggaga tctgcaacag tgacaatggg aagctgtggg gtgaaaacct
    18421 tttcggacca gtgatgtatg tcgatggcca aagcaggacc ctctacgcca actttcccga
    18481 taaggaaggc ctgctcagag agagagacgg agtttacacc ggagtcctcc cgaaagagag
    18541 aattattgca aacaatgtca tagagtttgg cggtgtgaaa tatgcaatgg taccccttcc
    18601 cgaaaaggat gacaggtacc gcatggtcac ccggaccatc cacagcctct tccactgcta
    18661 ccaggacaga catggcctga agccttccac cttcagcacc aggcacctca acgagaaaaa
    18721 cgccaggctt tacctcaagc ttgaatggaa agcacttaca aacgcaatca cctcttcagg
    18781 cgagaaacgt gaccaggcta tccgtgatgc cctggtcttc cgcggagcaa gacgcgaact
    18841 ctttccctca gcaataacag atgaaaataa gttcgagaac tttgaaggac tgaccacata
    18901 tacatatgta aagctgtgca cctcgggccc cgaagagatc agaacaagga tactcgaata
    18961 ccttgacagg gtctaccaga acagatcata tgcttttggc tacggattcg ttaacggagc
    19021 actttatgcc gtactgctga atgacaagga tttcgatttt aaacagatac agacccagga
    19081 ctatgacctg ggcaaagccg tgctcgacgc atacggagtt acgctgcctg ctgtctgccg
    19141 cgacgtggca ggatcacttg catttaacta tgatatccag gctatccgtg ctgaagagag
    19201 ccagcgcgag tcgaagataa atgagtacac ccgccggatt gtggcaacat tctatgaaaa
    19261 gccggtgctg acaatatcta tggagagccc caacttcagc ttcgagccgg aggatataga
    19321 ctacctcgat acgctgggca ccatgtataa gaaactccgt gtgtcagaca actgggggag
    19381 gctggcagtt gacaagggaa gtgcgcttat cacaaatgac ctgcgcactc tcagagtgag
    19441 cgcaaagaac ctgctggttg aaagaaacca tatatccggt gaagggtggc acctggtact
    19501 aaacgacgga tggcgtccgg aacagctgga taacggcaac tacacaatta ccagacatta
    19561 aataacagtg ctatttcttc aggtgggcca ggctctcctt tattgtagct atccgcgccc
    19621 gtgagtcagc ctctttcttc cgctcattat caagtacttt ctgaggagcc ttggaaataa
    19681 acttctcatt tgaaagcttc ttgagaacag aggccaggaa cccctcctga tagaccagtt
    19741 catcttccag cttgcggatc tcatcttcat tatcaatacc cttactcagg gggacaaaga
    19801 attcggatgt accaatcctg aacgaagctg ctccctcaac cttctcttcg gtaaccctga
    19861 tctcagagag gttgcacatg cggattaaga ccggatcaaa ctcatttccg gttgcaccgg
    19921 gagaggcgca tagaacaagg ggttcgcgca acgggagatt cttttccttc cttaccgtgc
    19981 gcacggcgct gactatctcg cgcaccgtgt caaaacgcgt cagcattccg gcctcgtaag
    20041 gtgcgggaag gggcatatct tcaaccatga tgctgcttcc ttcaggcctg tcagccagcc
    20101 tctgccatat ctcctcggtg ataaagggca tgaagggatg cagaatcttt atcaggtcac
    20161 caaacatctc cagtgtctca cgatgagtca caccgtcaat gggctt
//
LOCUS       DAOPBK010000022        19750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2862_length_19750_cov_5.229906, whole genome shotgun sequence.
ACCESSION   DAOPBK010000022 DAOPBK010000000
VERSION     DAOPBK010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19750
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_19750_cov_5.229906"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLA17_02360"
     CDS             complement(<1..225)
                     /locus_tag="PLA17_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84287.1"
                     /translation="MKAVMIVHNLALTEKVEFTLEHFGIRGYTHWDNVKGSGSFTGVP
                     HLGTHTWPEINGVTLTFVDDEKVALLLEAVR"
     gene            complement(222..3383)
                     /locus_tag="PLA17_02365"
     CDS             complement(222..3383)
                     /locus_tag="PLA17_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK84288.1"
                     /translation="MSIYASAVKKPVTTILIFVITMVLGLYSLTQLPIDLYPEVELPF
                     LSVYTTYNGASAADIETNITRPIEAALNSVSGLKEMTSRSMDNASVIFMEFEYETNLD
                     EASNDVRSMLSFVQGYLPEDAEDPAIFKFNSSMMPVIFYAITAQESYRGLEKILDEKL
                     VNPLNRIDGVGNVALSGVPGREIYVEVDPRRMEAYNLTIEQIGNVLRAENLNMPAGYL
                     EMGKTDYPIRIQGELAESDLISGLIVGNYNGNNIYIRDVATVNDTIRETKMVSKVDGE
                     QGMVLFVQKMSGANSVQICREVDRDIKKLQKDLPPDIRIEKIIDTSDFIQGSINNLTE
                     TLMYAALFVILVVLFFLGRWRATLIIILTIPISLLVAFVYIFITGSSINIISLSSLSI
                     AIGMVVDDAIVVLENITKHIERGSRPREAAIYATNEVWLAVIVTTLTIVAVFLPLTFV
                     KGLTGVLFSQLGWIVSITIIMSMLAAITLTPTLSALLLRLRPVRENAPIWTWDGSVYK
                     FLEWLDRFYVRTLSWVLHHKRFVVTAAILIFAGSMFLFRFIGTEFMPSADESRVSATI
                     ELQTGTRVSETEKVADRIYTLVKEQVPEIKLISMSAGSDDSGGFATLFSDGGTHVATF
                     TFALVDVEERKRSSREVAEQIRGIIAPMPEVINFSVLSESAGMGSMGGGNTVDIEIYG
                     FDLTRTNLLAEELAERVKKIPGATNVDISRDKSKPELQVILDQNKMLRYGLTTAQVSS
                     MIRNRVDGLIATRLRQFGDEYDIIVRYKESARNSLTELQNIGIATPAGQVIRLGEIAD
                     VKEHWSPPNIERKRKERIVTVSVTPYKRALNLIAADIQKEIAAVEIPSGVMVEISGAY
                     EDMMESFMDIGLLMVVAIILVYLVIASQFESLKMPLIIMLSIPFAFSGVAIALFLSGI
                     NLSLIAAIGAIMLIGIVVKNAIVLVDFINLTRDRGVELYQAVLISGRSRLRPVLMTSM
                     TTILAMIPLAINPGEGSELWQPMGVTVIGGLVFSTIVTMVIVPVGYVLMARHGERDRK
                     HKVVYRDMKFLDDIKENHENGKS"
     gene            complement(3410..4462)
                     /locus_tag="PLA17_02370"
     CDS             complement(3410..4462)
                     /locus_tag="PLA17_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK84289.1"
                     /translation="MNRTILSLIFAGGILLASCASKDQKAGASAASTADSVAAIPVKI
                     MPVSRTTIARTIDYTATIQAYEEVNLAPSTPGRVDQIYVEVGDRVQKGQKLFLMDRTQ
                     YYANKIQLANLEKDLARLDTLLKAGSVKEQLYDQTKAQYEIMKTNVEFMEVNTMLEAP
                     FSGIITGKYLENGELYSGVPGMSGKAAVVTLMQINPVKVIVNISEQYFPLIKNGMKAR
                     IMADVYPDKSFEGVIFRVHPTIDAMSRSFSTEIRINNGAEMLRPGMFARAYIDMGETE
                     AVVVPANTVLLQEGTNERFVFVVENDVALRKTVIIGQRFDDRFEIAGGDLKEGDNLVT
                     DGQARLKSGQKVEVSR"
     gene            complement(4491..5816)
                     /locus_tag="PLA17_02375"
     CDS             complement(4491..5816)
                     /locus_tag="PLA17_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK84290.1"
                     /translation="MMKRRLTAVFIILVVAVSATLRAQDTPQVLSLTVDQAVEYALDH
                     NRSVAAARYDLLASEKSVWETLAQGLPSIDASASLSYNLAIMTRIIDFGGTPTPFKFG
                     TKYDAAVGVSASTVVFNAPWLVGIETAKMAQVLARQGLTQTTLDIKENVRNVYYLILT
                     LKETMKVVERNLENLNEILASTKAMYSVGMAEATDVDQMQSTVTMLENSRSSMLRTLE
                     VNHNMLRFLLGVPANTEIILTDSIDGITAQIDVEALLADEFRLEDNISYQLIESQLAM
                     SELALKSAKASNLPTLAGTIYYNRTGMGDELMHMQYFPYSATAVQLQIPIFASGLRSA
                     KISKAKINLEKSINTKSMISEQLLMQERQLRYNLLNASEQFRSQKANIEIAGRVLESY
                     QNKYDQGMASSLDLTQANNNYLTAQNNYIQALMNLLQTKVEFDKLMNKL"
     gene            complement(5935..6552)
                     /locus_tag="PLA17_02380"
     CDS             complement(5935..6552)
                     /locus_tag="PLA17_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPK84291.1"
                     /translation="MSQMEEYNARIIAGAADLFRMYGIKSVTMDDIASRLGISKRSIY
                     ERFKDKDSLLFAVINSMIEKQREAIERILADSPDVITAIFTIIRMGRDHAAAMNPLIS
                     ADLKKYHSKVMQKLKEKCGHPDYESAGRILEKGISQGIFRSDIHVEIVSRAFRGIISL
                     TRNEELFPRERFMQRDLVRNIIVNFMRGISTPRGIELIDSMENEI"
     gene            complement(7109..8323)
                     /locus_tag="PLA17_02385"
     CDS             complement(7109..8323)
                     /locus_tag="PLA17_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPK84292.1"
                     /translation="MLRYLALIIIMLPFAVQAQITAPGSHAVRYTSYPATTRQDPIFI
                     FCAVNGSETGTLEAASPGGTAPFAFTWTRYNQSGGGYNIPVKSESGLTSTAAGLAEGG
                     YRVRITDGGGYSTELYAWVNIDIPVAGAALLHNTCDYVALDGTKAVDPFTYYDPLTNV
                     SRLLPNGVKHLWSSSPASAIPYPDLEIDPITFSPPLTDVRYTLQVTDSFGCSTSASFD
                     YTSIHVKAEFTADQNEGESPLEITFTDKSVRASKYLWRFGDDSVSTLKDPGTHTYYIP
                     GEYTVSLVIESELTCRDSATVKIKVLPSAMQIPNVFTPNDDGLNDYFVPDKKSIRFLN
                     LQIFAKSGHRVYYYQGDGDDLQNWLGWDGRINYSERKAEPGAYYYVLRAEGYDDVEYK
                     GREFRGTVYLYR"
     gene            8414..11017
                     /locus_tag="PLA17_02390"
     CDS             8414..11017
                     /locus_tag="PLA17_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPK84293.1"
                     /translation="MFRDYLKELNEAQQAAVVNTSGPAMVIAGAGSGKTRVLTYRIAH
                     LLQQGVKAHRILALTFTNKAAGEMKERIIRIVGPEKARYLWMGTFHSIFAKFLRYDGH
                     HLGYKSNFTIYDQDNTRSLIRTIIKELNLNDSVYKPSSVAGRISYAKNNLITARQYSS
                     DPQITESDKASRIPEMAAIYRHYAARCFKANAMDFDDLLMNINILFHDFPAVLEEYRN
                     RFDYILVDEYQDTNYAQYIIVHKLSENHRNLCVVGDDAQSIYSFRGARIENILNFKND
                     YPDYQLFKLEQNYRSTQTIVNAANSVIEKNAGQISKTIFSKNEKGEKIRVIQTTIDSE
                     EGSLTASDILDTRMRERLNWSDFAILYRTNAQSRIFEERLRKRNIPYKIYGGQSFYER
                     KEIRDLIAYLRLVVNPDDEEALRRIINYPRRGIGDTTIMKLMELARSLDMSIWSIISS
                     QELSAKYLAPALRAKVSHFTGLMLPVMAVAETATAYDIAFSMAAASGILNELKQGQVP
                     EEIDRLENVQELLNGIKEFTESAVTNGEPADIATWLQSVSLLTDQDMEKPEDRDKVTL
                     MTIHSAKGLEFKHVYIAGLEENLFPSAMNLFNPRELEEERRLFYVALTRACRRVTLSY
                     ALNRYKWGTLERSSPSRFIGEIESKFLCYPQTGGKPFGQRAFYEPGPAGIIPDQPLTF
                     SPTARMTRLRDLKAPSTAERVNGAGSAGNGAAGDTAGRSVTGHGGGRSKGMDGSGGAG
                     RCTGKGDSSTGTGMGSGTGMGGGTGLGGGAISTGTGNGGAGTEMGSGTGIGGGAGMGG
                     STGMDGSGGAISTSINLVLEPGDRVVHERFGEGEVISVEGESPNTTALVNFKSSGTRK
                     LLLRFAKLKRI"
     gene            complement(11179..11955)
                     /locus_tag="PLA17_02395"
     CDS             complement(11179..11955)
                     /locus_tag="PLA17_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84294.1"
                     /translation="MEAKRIYRYVPSFSGAYGTGWKAMADNFLRLLLVVLVLVVIGSP
                     MLGSNTNWEFDPGDFSNIPFDVTDFFKIGAIGVAAAFLGLIALLWFFLLIPVFNYGAK
                     MMFVQAARQITPEFDMLISGFRKNYLNIVLANLLLTALIGIGIVCLIVPGIIIACRLA
                     FTPYLVMDRNLDPIRAAEESWRLTRGHGWTIFLMGFISIFIYIAGFICFLVGVLVSDM
                     WVKSSFAALYHSVLIEKGLLQAESVEVTEPVQGDAAANEL"
     gene            12128..13078
                     /locus_tag="PLA17_02400"
     CDS             12128..13078
                     /locus_tag="PLA17_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK84295.1"
                     /translation="MEFISLVIFIFSAAMAAGSILLVTRLLEEFAHPCFRSLFYHNLF
                     ISAFGFYGIWGQFIIMALAGSRLDPEIFSTISVLSLLPGLPFLVFAWMMLIRFAVEAS
                     GHKLRNWFTVVFLIVNFGIIAALSLVIRDMSFNDALPLFKYYYIAAALLFATMAATLI
                     VRGDSVSPGRSDRIALALIITGGALAQSLSLLLIHHSAWMAMAFVFLLFAAITLMSLY
                     LSYRADLKPVRLAEPLPLPNGFEDFIRKNEISPREAEIITEICNGLSNQEIADKLFIS
                     LQTVKDHTSRIYMKTNVRNRMQLMALVRGMDTGTAAGEHL"
     gene            complement(13131..13997)
                     /locus_tag="PLA17_02405"
     CDS             complement(13131..13997)
                     /locus_tag="PLA17_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPK84296.1"
                     /translation="MAPKDSAEAKDIKSSMAGYISVSLELLAMQPVPDDAAIHDVRVL
                     MKKYRAALRLVKPILNEAVYRREYLAGRETGRLLAQWRESAVLRKTAKALKKDNPELF
                     IRLDGNEKIQKLLRKSYSTWEQAGDSAKLVHEVAERLKKTHYRLRFLSLNEPDMRLMQ
                     GELGRSHAAASAAYLACRNKPVARLLHDFRKKSKTLMYQLTFFRHLAPKSVRQLEKRL
                     GVMTQNLGKYNDLAQLMLLTEYRYGDPENSDADNELAVVIRDRQDRYLAKVWPLAYQI
                     FAPGKKLEVLPG"
     gene            14156..15208
                     /locus_tag="PLA17_02410"
     CDS             14156..15208
                     /locus_tag="PLA17_02410"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPK84297.1"
                     /translation="MGKSNGRNSKPRTIGILTAGGDCPGINAAIRGVGKTAIVKYGMK
                     VIGISDGYAGLINKEYRELTESDLSGILTLGGTILGTSREKPYKGNGKKKDESRKPLL
                     IKEHYEQMGLDCLVCIGGNGTMKTANLLSEEGLNVVGVPKTIDNDIWGTDITFGFDSA
                     VNIATEAIDRLHSTANSHKRIMVIEIMGHNAGWLALYAGMAGGGDVILIPEIPYNEKV
                     VIDYLCERADNNKPYSIVVVAEGVEIPEGEDSPARYLAGRIQKGTGLETRETVLGYVQ
                     RGGSPSPMDRVLASRFGAAAANLIARGEFGRMVAQRNGIITSVKLSEVGGKTKLVKSD
                     DPMVRQAREMGTCFGD"
     gene            15283..15813
                     /locus_tag="PLA17_02415"
     CDS             15283..15813
                     /locus_tag="PLA17_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84298.1"
                     /translation="MVKKINKDASETRKLIFIRHSKAESYSSEISDFERSLTMKGKVY
                     SRLMAEILKAKGEDPGRIISSPAFRSLETAIIFCRGYGLNMDEIKLIPELYDGMREEE
                     FLPFIRKQNDKDHTITIFGHNPMITEMAAYFACDEPRSIAKTGIYCLAFDVASWSEVE
                     PESGRTLYYLTPSDLS"
     gene            15810..17858
                     /gene="ppk1"
                     /locus_tag="PLA17_02420"
     CDS             15810..17858
                     /gene="ppk1"
                     /locus_tag="PLA17_02420"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPK84299.1"
                     /translation="MNKAYIPKEISWLSFNARVLQEAANNNVPLLERFNFLGIYSNNL
                     DEYFRVRVATLRRLAFFSNKARNILGYCPKAALKSIQETVLRQNEIFEKTYSDLLDEL
                     KKHQIHFVNEEQLDDAQTEFVRNYFRKEVRSRLMPLIISKDNKLPNLTDDAIYLAIQL
                     RNSETGKKRYALLEIPPAGILPRFVMLPGKKGMRYVIFLDDVIRWGLKEIFSIMPYDE
                     ITAYTIKVTRDAELEIADDISESYIDKISRSLQQRKKGSPVRFVHDREIPEEFLKILS
                     KKLNLGSEDVIIPGNRYHNFKDFMKFIEIEGEGLNYPKLPPVRHPALQYGKSILSVIM
                     KRDIMFYFPYHPFDHFIDLLREASIDPSVVSIHITLYRLARNSSVINALMNAARNGKS
                     VTTVVELQARFDEEANIHWGNKLLDEGVKVIYGVPGLKVHSKLCLITRVKGRTTQRYA
                     AVGTGNFNEDTARVYTDHLLLTTEKKITNEVLKVFNFFNANYRKDKFYHLLVSPFGLR
                     NRLMIMIENETKNAKKGKEAYIKIKINNLTDEDIISRLYKAADAGVQIRLIVRGMFSM
                     APGAKGVSENIQAIGIVDRFLEHTRFMIFCNGGDELVYIASADIMPRNLDRRIEVTCP
                     VWDPAIKKEINDIFNIQWNDNVKARILDAELSNTMVSNGEPPLRSQTEIHRYFSGIGS
                     "
     assembly_gap    17867..17966
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18001..19605)
                     /locus_tag="PLA17_02425"
     CDS             complement(18001..19605)
                     /locus_tag="PLA17_02425"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPK84300.1"
                     /translation="MKNYLLPKIFLLVLITVFSCSRGEKKGTGLANSFRFEEYTIMQL
                     QQGYDKGEYTVTDVVQAYLDRISAIDDNGPTLNAVIEVNPDALAIAAQLDAELKEGRK
                     RGPMHGVPVLLKDNIDTHDQMSTTAGSRALSGSRPLQDSYIAMKLREAGAVIIGKANL
                     SEWANFRGNLSSSGWSGVGGQTKNPYVLDRNPCGSSSGSGVAVSANLCMISIGTETNG
                     SIVCPSTANGIVGIKPTVGLLSRSGIIPISFTQDTPGPMGRTVSDAAAALGAMTGIDP
                     NDSKTAASEGKYLTDYTPFLKEGGLEGKRLGWYTSARGTHFKVDTLMQHAVDFLKSQG
                     AEVIEIDNIYSREVGSLSFQVMLYEFKDGLNKYFASLGDNAPVKSVEELIEFNRNDSI
                     ELRYYDQELLIEAQNKGDLTSREYLDALEKMNRLVRDEGIDKVMKEYNLDAFIAPTGS
                     PAWKTDLVNGDSYTVSSSSPAAVAGYPNVTVPMGYVDGLPVGISFFGRAWSEPLLIEI
                     AYSYEQGTKYRRAPQFLPTLPFEAEN"
BASE COUNT         4820 a         4972 c         4965 g         4893 t
ORIGIN      
        1 ccgtaccgcc tccagcagga gggcaacctt ttcatcatct acgaaggtga gagtcacccc
       61 gttgatctcg ggccaggtat gggtgcccag gtggggcacg ccggtaaatg atccggaacc
      121 tttaacatta tcccagtgag tatatcccct gataccaaaa tgttccagcg tgaactctac
      181 cttctccgtc agggcaaggt tatgaactat cattactgct ttcatgactt tccgttttcg
      241 tggttttcct taatgtcgtc gagaaacttc atgtccctgt ataccacctt gtgtttcctg
      301 tccctttcgc cgtggcgggc cataagaaca taacccaccg gcacaatcac catcgtcaca
      361 atagtggaga acaccagacc accgataact gtaacaccca tcggctgcca tagctccgaa
      421 ccctcgcccg ggttgatggc cagcgggatc atggccagaa tggtggtcat ggaggtcatg
      481 agcacaggcc tgagcctgga acgtcctgat atcagaactg cctgataaag ctccacgcct
      541 ctgtcacgcg tgaggttgat gaagtcaaca agcacgatgg cgttcttgac tacgataccg
      601 atcagcatta tggcgccgat tgcggcgatg aggctgaggt tgattcctga cagaaacagc
      661 gctatagcca ctccggagaa ggcaaacggt atcgagagca tgatgatcag cggcatcttg
      721 agcgactcaa actgggatgc gattaccagg tagacaagta tgatcgcaac aaccatcagc
      781 agccctatgt ccatgaatga ttccatcatg tcctcatagg cgccggagat ctcaaccatc
      841 acgcctgatg gaatctccac agcggcaatt tctttctgga tatcggcagc aataaggtta
      901 agcgccctct tgtagggtgt caccgaaacg gtcacaatgc gctccttccg ctttctctca
      961 atgttgggtg gtgaccagtg ttcctttaca tcggctattt cgccgagcct gatcacctgt
     1021 cctgcaggag tggctatgcc gatattctgc agctcggtga gcgagttgcg ggctgactcc
     1081 ttgtacctga caatgatatc atactcatca ccgaactggc ggagacgggt ggcgatcagg
     1141 ccatcaacac ggttcctgat cattgatgaa acctgagctg ttgtcagtcc gtacctgagc
     1201 atcttattct ggtcaagtat cacctggagt tccggtttcg acttgtcacg gctgatatca
     1261 acatttgtgg cgccgggaat ctttttaacc ctctctgcca gctcttccgc caggaggttg
     1321 gtccgggtga gatcaaatcc gtatatctct atgtcaacgg tgtttcctcc tcccatggag
     1381 cccatgccag ctgattccga gagtaccgaa aaatttatca cctcaggcat cggggcaatt
     1441 ataccccgta tctgctctgc cacctccctg gaagacctct tcctctcttc cacgtcaaca
     1501 agtgcaaatg tgaaagtggc aacatgggta ccgccatcgc tgaagagcgt ggcgaatccg
     1561 ccgctgtcat ctgagcctgc tgacatggag ataagcttta tctcgggtac ctgttccttc
     1621 acaagcgtat agattctgtc tgccaccttt tcggtttcgg agaccctggt ccctgtctga
     1681 agctcaatcg tggcagagac tctagattcg tcagctgacg gcatgaattc tgtgcctatg
     1741 aacctgaaga ggaacattga gccggcaaag atcagtatag cggctgtaac gacaaatctc
     1801 ttgtggtgaa gcacccagga gagtgtcctg acataaaatc tgtcgagcca ctcaaggaat
     1861 ttgtagaccg agccgtccca tgtccatatt ggtgcatttt ccctgaccgg tctcagcctg
     1921 agcaacaggg cgctcagcgt cggggtgagt gtaatggcgg ccagcattga cattatgatt
     1981 gtgattgaca caatccatcc aagctgtgag aagagcaccc cggtaagtcc cttgacgaat
     2041 gtcaggggaa ggaacactgc cacaatggtc agcgtagtga caataacagc cagccacacc
     2101 tcattggtgg catagattgc ggcctcccgc ggccggctgc cgcgctcgat atgctttgtt
     2161 atgttctcaa gcacaacaat ggcatcatca accaccatac cgatggcaat cgagagtgac
     2221 gagagcgaga ttatgtttat tgagcttccc gtgatgaata tgtacacgaa ggctaccagc
     2281 agcgatatgg ggatagtcag gatgattatg agggtggccc gccatctgcc caggaaaaac
     2341 agcactacca gtatcacgaa gagagctgcg tacatcagtg tctccgtaag gttattgatt
     2401 gaaccctgta taaaatccga tgtatcaatg atcttttcaa tgcggatatc aggcggaagg
     2461 tctttctgaa gcttttttat gtcacggtct acctcgcggc atatctgaac actgtttgct
     2521 cctgacatct tctggacgaa gagaaccatt ccctgttcac catcaacctt tgacaccatc
     2581 ttggtctccc tgattgtgtc attaacagta gcaacatccc taatgtatat gttgttacca
     2641 ttatagttgc cgacaatcag accggaaatc aggtcacttt cagccagctc gccctgtatt
     2701 ctgatcgggt agtcagtctt gcccatctcg aggtatcctg caggcatgtt caggttctca
     2761 gcccttagca cgttgccaat ctgctctatt gtaaggttat aggcctccat tctcctcggg
     2821 tccacttcaa catatatctc cctgccgggc actccggaga gcgctacgtt tccaactcca
     2881 tcaatacggt tgagcgggtt aaccagtttc tcgtcgagaa tcttctccag cccccggtaa
     2941 ctctcctgtg ccgtgatggc ataaaagatg acgggcatca tgctcgagtt gaacttgaag
     3001 atggcagggt cttcagcatc ttccggaagg tagccctgaa cgaatgaaag catgcttcgc
     3061 acatcgttgg aagcttcgtc aaggttggtc tcgtactcaa actccatgaa gataacagat
     3121 gcgttgtcca ttgaccgtga ggtcatttcc ttcagtccgg agacggagtt gagtgcggcc
     3181 tcaatgggac gggtaatgtt tgtttcaata tccgctgcac tggctccatt gtaggtagtg
     3241 taaactgaca ggaaggggag ctctacctca gggtagaggt ctattggcag ctgcgtgaga
     3301 gaataaagcc ctagtaccat cgtgatcaca aagatcagta tagtggttac gggctttttt
     3361 actgcactgg catatatact catattctat aattgtctct ggttaatcat catcttgata
     3421 cctctacctt ctggccgctt ttgagacgtg cctggccgtc tgttacaagg ttgtctccct
     3481 ccttcaggtc gccgccggcg atctcgaaac ggtcatcaaa cctctggcca attatgacag
     3541 ttttgcgcag tgccacatcg ttttcaacaa caaagacgaa tctttcgttg gtgccctcct
     3601 gcagaaggac agtatttgcc ggaacaacca cagcctctgt ctcgcccatg tcaatgtatg
     3661 cacgtgcgaa catgcccgga cgaagcatct ctgcgccgtt attaatccgt atttcggtac
     3721 tgaatgagcg tgacatggca tctattgtgg gatgaaccct gaagatgaca ccttcaaagc
     3781 ttttgtcagg gtatacatct gccatgatac gcgctttcat tccgttcttt atcagaggga
     3841 aatactgctc ggagatgttg acaataacct tgaccgggtt tatctgcatc agagtgacca
     3901 cggcagcctt gcctgacatg ccgggcactc ccgaatagag ttctccgttc tcaaggtact
     3961 tgcctgttat gattccggaa aacggagcct caagcatggt gttcacctcc atgaattcca
     4021 cgttcgtctt cataatctcg tattgtgctt tcgtctggtc atagagctgc tctttcacgc
     4081 ttcctgcctt aaggagggtg tcaagccggg ccaggtcctt ctcaaggttt gccagctgaa
     4141 tcttgtttgc ataatactgt gtgcggtcca tcaggaagag cttctgaccc ttctgcaccc
     4201 tgtcacccac ttcgacgtaa atctggtcaa ccctgcccgg ggttgacgga gcgaggttaa
     4261 cctcctcgta tgcctgaatt gtagctgtat agtctattgt tcttgcaatg gtggtcctgc
     4321 tgaccggcat gatcttaacc gggatggctg caactgagtc tgcagttgat gccgcagagg
     4381 ctccggcctt ttgatccttt gaagcacatg atgcaagcaa aatgcctccg gcaaagatca
     4441 gagaaagaat tgtgcggttc attgtattct gttttattgt tgtttttctt ttacagtttg
     4501 ttcataagct tgtcaaattc caccttggtc tgcaggaggt tcataagagc ctggatatag
     4561 ttgttctgtg cagtcaggta gttattattg gcctgtgtca ggtccaggct tgaagccatc
     4621 ccctggtcat acttgttctg atagctttcc agaacccgtc cggcaatctc tatgtttgcc
     4681 ttctggctcc tgaactgttc actcgcgtta agcagattgt accgtaactg cctctcctgc
     4741 ataagcaact gctcggaaat cattgatttt gtattgatac tcttttcaag attgatcttc
     4801 gccttgctga tcttcgcact cctcagtccg gatgcgaata tcggaatctg gagctgtact
     4861 gcagttgccg aataggggaa gtattgcatg tgcatcagct catctcccat accggttctg
     4921 ttgtagtaga tagtacctgc aagagttgga aggttactgg ccttcgcgct tttaagcgcc
     4981 agctccgaca tggccagctg gctctcaata agctggtagc tgatgttgtc ttccagcctg
     5041 aactcgtcgg caagaagggc ttcaacatca atctgagccg ttattccgtc aatgctgtca
     5101 gtgagaatta tctcagtgtt tgcaggaact ccgagaagga accgcagcat attatgatta
     5161 acctccaggg tgcgaagcat tgacgaacgt gagttttcaa gcatggtaac ggtcgactgc
     5221 atctgatcca catcagttgc ttcggccatg ccaacactat acatggcctt tgtggatgca
     5281 agaatttcgt tcaggttttc caggttcctc tccacgactt tcattgtctc tttcagtgtg
     5341 aggatcaggt agtatacgtt cctgacattt tccttgatgt caagtgttgt ctgcgtgagt
     5401 ccctggcgtg caagtacctg agccatcttt gcggtctcga tgcccaccag ccatggtgcg
     5461 ttgaatacaa ctgtcgatgc cgatacccct acggctgcgt catacttggt accgaacttg
     5521 aaaggagttg gcgtgccgcc gaaatcaatg atccttgtca tgatggcaag gttataactc
     5581 agggaggcgc tggcgtctat tgaaggaagc ccctgagcca gggtttccca gacactcttc
     5641 tccgatgcaa gcaggtcata ccgtgcagcg gccacgctcc tgttgtggtc aagggcatac
     5701 tccaccgcct gatccaccgt cagcgagaga acctgcgggg tatcctgcgc ccgcagcgtg
     5761 gcagagactg caacgacaag aattataaaa acagctgtta atcttctttt catcataata
     5821 tttggtttaa aaggtatgat ggagcccaca tgactgaggt ttatgtaaac aggtgtttgt
     5881 ctgtttgata catgtgggtt tcatatagtt gtcctttatg ttgctgtgtc agaatcatat
     5941 ttcattttcc atcgaatcga tcagttcaat accccgggga gttgatatgc ccctcataaa
     6001 gttcactatg atgttccgca cgaggtccct ctgcatgaat ctttcgcggg ggaagagctc
     6061 ctcgttgcgt gtcagtgaga ttattcccct gaaagccctg cttacaatct ccacatgaat
     6121 atcactcctg aagataccct ggctgatccc cttctccagt atcctgccgg ccgattcata
     6181 gtcgggatgt ccgcatttct ccttcagttt ctgcattacc ttgctgtggt atttcttcag
     6241 gtcggcgctg ataagaggat tcatggctgc ggcatgatcc cttcccatcc ggataatagt
     6301 gaagatggca gtgattacat caggtgagtc tgccagaatt ctttcaattg cttctctctg
     6361 tttctcgatc atcgaattga tcactgcaaa cagcagggaa tccttgtctt tgaacctctc
     6421 atagatgctt cttttcgaga tcccgaggcg ggaagcgata tcatccatgg tgactgactt
     6481 aatgccgtac atccggaaca gatctgccgc accggctata atcctggcat tatattcttc
     6541 catctggctc ataatatttt tggaaacagg gcgcaatatt ctgaaaacta tttgagtttt
     6601 cctagttttt aatgtcaaat gtttgttaaa ttctgttgag ttgcggtaaa tggcaggcga
     6661 acggagaatg gtcatcaggg ggcataccgc cgttgtacaa tggtgcaagc cctgtgggct
     6721 gttcattgcc agcactgctg cctgtggact attcaatgcc cacactgccg gatactgtct
     6781 ggattgatta ctgattgcgg actatcatct gccggctgtt cattaccaat actgttgact
     6841 accgggtgat gattgctgac tgctgattat cgccagccgg ttgctcgtgg tcaaaactgc
     6901 ggccggctgt tcattaccaa tactgttgac taccggatga tgattgctgg ctgctgatta
     6961 tcgccagccg gttgctcgtg gtcaaaactg cggccggccg ttcattacta acaccgctgc
     7021 tgccggctga attgactgct gaatgcaggc tttcgactgg ctgttgcctg aattgactgc
     7081 cggctgaatt gactgctgac tgctgttcct acctgtacag atagaccgtc ccccggaact
     7141 cccgcccctt gtattcgaca tcatcatatc cctcagcgcg aaggacatag tagtatgcgc
     7201 cgggctccgc cttcctttct gagtagttga tacggccatc ccaccccagc cagttctgga
     7261 gatcatcacc gtcgccctga tagtagtaga ccctgtgacc gctcttagcg aagatctgca
     7321 ggttcaggaa acgtattgat ttcttgtcgg gcacaaaata atcattgaga ccgtcgtcat
     7381 tgggggtgaa cacattggga atctgcatcg ccgatggcag aacctttatc ttcaccgtgg
     7441 cagagtcacg gcatgttagc tcgctctcta taacgagtga gacagtgtac tctcccggta
     7501 tataataagt atgagtgccc ggatccttca gcgttgatac cgaatcatca ccgaacctcc
     7561 agagatactt tgatgccctg acagatttat cagtgaatgt gatctccagc ggtgactctc
     7621 cctcattctg atcggccgta aactcggctt tgacatgaat ggaggtatag tcgaacgatg
     7681 cagaggttga gcatccgaag ctgtcagtga cctgtagcgt atacctcaca tcagtcagcg
     7741 gcggcgagaa tgttatggga tcaatctcaa gatcgggata tggtattgcc gatgccggcg
     7801 aagatgacca gaggtgcttc accccatttg gcagcagcct tgagacattg gtcaacggat
     7861 cataatatgt aaagggatca accgcttttg ttccgtcgag ggcaacataa tcacatgtgt
     7921 tgtgcagcag tgcagcgccc gcaacaggta tgtcgatgtt cacccatgca tagagctcgg
     7981 tgctgtatcc tccgccatcg gtgatccgga ccctgtatcc tccttctgcc agacctgcgg
     8041 ctgtcgatgt caatcctgac tcactcttca cgggtatgtt gtaaccgcct cctgactggt
     8101 tgtatctggt ccatgtaaag gcaaagggtg cggttcctcc cgggctggca gcctcaaggg
     8161 taccggtctc acttccattg actgcacaga agataaaaat cgggtcttgc ctggtggtgg
     8221 ccggatagga ggtataacgc acggcatggc tgcccggggc agtgatctgc gcctgcaccg
     8281 caaacggcaa catgatgatt ataagggcca ggtatcttaa cacacctagt taacggattt
     8341 actcattgat atgatatgca aacctaataa aaaatgccgt tctttggggg attaaaattc
     8401 tgcactctca ttcatgtttc gcgactatct gaaagagctt aacgaggcac agcaggcggc
     8461 cgttgtcaac acatcaggtc cggccatggt cattgccgga gcgggatcag gaaaaaccag
     8521 ggtattgact taccgtattg cccatctgct ccagcagggt gttaaggctc accgtatact
     8581 ggccctgacc ttcaccaata aggcagccgg agagatgaag gagcggataa tcaggatcgt
     8641 cggccccgaa aaggcacgat acctgtggat gggaacattt cattcaatct tcgcgaaatt
     8701 cctccgttat gacggccacc acctgggata caaaagcaac ttcacaatat atgaccagga
     8761 taacacccga agcctgatac ggacgataat aaaggaactg aacctgaatg actcggtcta
     8821 caagccctca tctgtcgccg gtcgcatatc atacgccaag aacaacctta tcaccgcccg
     8881 gcaatacagt tcggacccac agattaccga gtcagacaaa gcgtcgcgca taccggaaat
     8941 ggctgccata tacaggcact atgcagccag gtgcttcaag gcgaatgcca tggacttcga
     9001 tgacctgctg atgaacataa acatcctgtt ccatgacttt cctgcagtgc tggaggagta
     9061 caggaaccgg ttcgactata ttctggttga cgaataccag gataccaact atgcccagta
     9121 tatcatcgtc cacaaactgt cagagaatca caggaacctg tgcgtggtgg gtgatgacgc
     9181 gcagagcata tactcattcc gcggagcccg gatagagaat atcctgaatt tcaaaaacga
     9241 ttaccccgac taccagctgt tcaaactgga gcagaactac cggtcaacgc agaccatagt
     9301 caatgctgcc aacagcgtca tagagaagaa cgccggccag atcagcaaga ccatcttctc
     9361 caaaaacgag aagggcgaaa agatcagggt cattcagaca acaattgaca gcgaggaggg
     9421 cagcctgaca gcttcagaca ttcttgacac acggatgcgc gagaggctta actggagtga
     9481 ttttgcaata ctttaccgca ccaatgccca gtcgcgtata tttgaagaga gactgcggaa
     9541 gcgcaacatt ccctataaga tatacggcgg acagtcattt tatgagcgta aggagatacg
     9601 tgacctgatc gcttacctga ggcttgttgt caacccggac gacgaagagg cactcaggcg
     9661 cattatcaac tatccgagaa ggggcatcgg tgacactacc ataatgaagc tgatggagct
     9721 tgcccgttca cttgatatga gcatctggtc aataatctct tcacaggagc tgagcgcgaa
     9781 ataccttgct cctgccctgc gggcgaaggt gagccacttc accgggctga tgctgcccgt
     9841 gatggccgtg gcggagacgg ccactgctta tgacatagca ttcagcatgg ctgccgcaag
     9901 cgggatcctc aacgagctga aacagggaca ggtacccgaa gagattgaca ggctagagaa
     9961 cgtgcaggaa ctgctgaacg gtatcaagga gttcaccgag tcagcagtca caaacggaga
    10021 accggccgac attgccacat ggctgcagtc ggtctccctg ctcactgacc aggacatgga
    10081 gaaacctgaa gaccgcgaca aggtcacgct gatgaccatc cattctgcca aaggtctgga
    10141 attcaagcat gtatatatag caggccttga agagaatctc tttccctcag caatgaatct
    10201 tttcaatccg cgtgagctcg aggaggagcg ccggctcttc tatgttgcac tgactcgtgc
    10261 ctgccggcgg gtaacgctca gctatgcgct caacaggtac aagtggggca ctcttgagag
    10321 aagctcacca agccgtttca taggtgagat agagagcaag ttcctgtgtt acccgcagac
    10381 gggaggaaag cctttcggcc agagggcatt ttatgaaccg gggcctgccg ggataatccc
    10441 tgatcagccc ctcaccttct cccctacggc aaggatgacc aggctgcgtg atttgaaggc
    10501 gccttcgacg gcagaacggg tgaatggcgc aggctctgcc ggcaacggag cagcgggtga
    10561 caccgccgga aggagcgtaa cgggtcatgg tggcggcaga agcaaaggga tggatggcag
    10621 cggcggcgcc ggcagatgca cggggaaggg tgacagcagc actggcacag ggatgggttc
    10681 cggcaccggg atgggtggcg gtacaggatt gggtggcggc gccatcagca cagggactgg
    10741 taacggcggc gctggcacgg agatgggttc cggcaccggg ataggtggtg gcgccgggat
    10801 gggtggcagc accgggatgg atggcagcgg cggcgccatc agcaccagca taaacctggt
    10861 gctggagccc ggagacaggg tggttcacga acggttcggc gagggcgagg taatctccgt
    10921 tgagggcgaa tcaccaaata caactgcact tgtcaatttc aaatcctccg gaaccaggaa
    10981 gctcctgctt cgctttgcga aactgaaacg gatttagagc agattactgc cggcagcagg
    11041 attaactctc catcttcgtc ggagtctcaa actgccgatt gccagtctgg cacttctgac
    11101 taaaacaaag gggaccgtca tggctgaccg gccccctgtg aagattatga agttcaaata
    11161 gatggatact cagtagaatt acagctcatt tgcggcggca tctccctgca ccggttcggt
    11221 tacctctaca ctctccgcct gcaaaagacc tttctcaatc agtactgagt gatagagagc
    11281 tgcaaatgag ctcttcaccc acatgtctga caccagaaca cccaccagga agcagatgaa
    11341 acctgctata tagatgaata tggagatgaa gcccatcagg aagatggtcc atccgtggcc
    11401 gcgcgtcagt ctccagctct cctccgctgc cctgatcgga tcaaggttcc tgtccatcac
    11461 aaggtaaggg gtgaatgcca gacggcatgc gatgataatg ccgggcacta tcagacagac
    11521 gattccgatc cctatcagtg cagtcagaag aaggttggcc agcactatat taagataatt
    11581 cttccggaat ccgctgataa gcatgtcgaa ttcaggggtt atctgccttg cagcctgtac
    11641 gaacatcatc tttgccccgt aattgaagac cgggattagc aggaaaaacc agagcagtgc
    11701 gatgagtccg aggaaggcag cagccacccc gatagcgcct atcttaaaga agtcagtcac
    11761 atcaaatggg atattgctga aatcgccggg atcaaactcc cagttcgtat ttgaccccag
    11821 catcggggat ccgatcacaa ccaggaccag aaccacgagc agtaacctga gaaaattgtc
    11881 agccattgcc ttccatccgg tgccgtaagc accgctgaaa gaaggtacat acctgtaaat
    11941 tctttttgcc tccatgattt tgtttgcttt ttactgtttt gaataatttt tttgtcatca
    12001 caaatctatt atggaaccct cggacggaat tcatacctta gtagtgattt taaaaaacac
    12061 tacttaagta tgccaccccc tacttaagta gtatctgtaa atcaatgaaa tagataaata
    12121 ttcccgaatg gaattcatca gtctggtcat ttttattttc tctgcagcga tggctgcagg
    12181 gagtattttg cttgtcacca ggctccttga ggagtttgct cacccctgct tcaggtcgct
    12241 tttctaccac aacctgttta tttccgcatt tggcttttat ggaatatggg ggcaattcat
    12301 cattatggcg ctggcgggca gcaggctgga ccctgaaatc ttttcgacaa tatcagtttt
    12361 gtcactgctg ccgggattgc ccttcctggt gttcgcatgg atgatgctca tcaggtttgc
    12421 tgtggaagca tcgggacata agctacggaa ctggttcacc gtggttttcc tgattgtcaa
    12481 ctttgggatt atcgcggctt taagcctggt tatcagggac atgagcttca atgatgcttt
    12541 gcccctcttc aaatattatt atattgctgc agcgctcctc ttcgccacca tggcagccac
    12601 cctgattgtg cgtggtgatt cagtctctcc cggccgcagt gaccgtatag ctctcgcatt
    12661 gattataaca ggaggcgcat tggcccagtc cctctccctg ttactgattc accactccgc
    12721 ctggatggcg atggcatttg ttttccttct ctttgcagcc attaccctca tgtcattata
    12781 cctcagctac agagctgacc tgaagcctgt caggctagct gaaccactgc cactgccaaa
    12841 tggctttgag gatttcataa gaaaaaacga gatatctccg cgcgaagcag aaattatcac
    12901 tgaaatatgc aacggcctga gtaaccagga gatagccgac aagctcttca tcagcctgca
    12961 gacggtgaag gaccacacct cgcgcatcta catgaagacc aatgtaagga acaggatgca
    13021 gctgatggcg ctggtaaggg gaatggatac cggcactgcg gcgggagaac acctttagcc
    13081 tggcagcacc tgcaattgcc ggataccggc actgcggcgg gagaaggcct tcagccaggc
    13141 agtacctcca gtttctttcc cggagcaaat atctggtatg ccaatggcca cacctttgca
    13201 aggtacctgt cctgcctgtc cctgatgaca acagccagtt cattgtccgc atcactgttt
    13261 tcagggtcac cgtaccggta ctccgtaagg agcattaact gtgcaaggtc attgtatttt
    13321 cccaggttct gtgtcataac cccgagtctc ttctcgagct gcctgacact ctttggcgcc
    13381 aggtgcctga agaaggtcag ctgatacatg agtgtcttcg actttttccg gaaatcatgc
    13441 agaagccggg ctacaggttt gttgcgacag gccaggtaag cggccgaagc agcggcatga
    13501 ctcctcccga gttcaccctg catcagccgc atgtccggct cattcagact caggaacctg
    13561 agcctgtagt gtgtcttttt cagtctttcg gcgacttcat gtaccagttt tgcactatca
    13621 ccggcctgtt cccatgttga ataggatttg cgcagcagtt tctgaatctt ctcattcccg
    13681 tcaagcctga taaaaagctc cggattatcc ttcttcagtg ccttcgccgt tttgcgaagc
    13741 acggcactct ccctccactg tgccagcagg cgtcctgttt cccgtccggc gagatactcc
    13801 ctgcgataaa ccgcctcatt cagtattggc tttacaagtc ttaaagcggc acggtacttc
    13861 ttcatcagca ccctgacatc atggattgca gcgtcatcgg gcactggctg catcgccaga
    13921 agctccagcg aaacggaaat atatccggcc atcgaacttt ttatatcctt cgcctcggca
    13981 gaatctttag gggccatgat cagtaaattg attggtgcta aagttacata aatgtaacgg
    14041 gtttcctgtc ttttatcctt tccagtggtt tatctttgac actgaccagg ttgcaaatga
    14101 ccggataaag ggtctggctt tttcgtacat ttgatgtggc aaaactttaa caacaatggg
    14161 caaatcaaac ggcaggaaca gtaagccccg caccataggt atcctcaccg cgggaggcga
    14221 ctgtccgggt atcaacgcag ccatcagggg cgttggaaag acagccatag tcaagtacgg
    14281 tatgaaggtc atcggcatca gtgacggcta tgccggactg ataaacaagg agtaccggga
    14341 actgacggag agtgatctct caggcatcct gacccttggg ggaaccatcc tgggcacttc
    14401 acgcgagaaa ccttataagg gaaatggcaa aaaaaaggac gagtcgcgaa agccacttct
    14461 gataaaggag cattatgagc agatggggct cgactgcctg gtatgcatcg gcggcaacgg
    14521 cacgatgaag acggccaatt tactttcaga agagggcctg aatgttgtag gtgttcccaa
    14581 aaccattgac aatgacatct gggggactga tattactttc ggattcgact ccgcggtaaa
    14641 cattgccact gaagccattg accggctgca ctccacggcc aactcgcaca agcgtatcat
    14701 ggtgattgag ataatggggc acaatgccgg ctggctggcg ctctatgccg gaatggccgg
    14761 cggaggagat gtcatactca ttcctgagat tccctataat gaaaaggttg tgatagacta
    14821 tctctgcgaa agggctgata acaacaaacc ttactccatc gttgttgtgg ctgagggcgt
    14881 tgagataccg gaaggagaag actctccggc aaggtacctg gcagggagga ttcagaaggg
    14941 aaccggtctg gaaacaagag agacggttct tggttatgtg cagagaggcg gatcgccatc
    15001 acccatggac agggtgctgg cttcaaggtt cggcgcagcc gcggcaaacc tgattgccag
    15061 gggcgaattc ggcaggatgg tggcacagcg caacggtata ataacctcgg tgaagctttc
    15121 ggaggtgggc gggaaaacca agctcgtaaa gagtgatgac ccgatggtca ggcaggccag
    15181 ggagatggga acctgtttcg gcgattgaca catgaacatg acatggtgac acggaaccgg
    15241 ggtgcggtta cctgaaacag caaatcagat acgattcaaa atatggtgaa gaagattaat
    15301 aaagatgcaa gcgagacaag aaaactgatt tttataagac acagcaaggc agagagttat
    15361 tcctccgaaa tctccgattt tgagagatcg ctgacgatga aggggaaagt gtactcaaga
    15421 ctgatggcag agattctcaa ggcaaaaggc gaagacccgg gaagaattat cagcagtcct
    15481 gcattcaggt cactggagac agctatcatt ttctgcaggg gatacggcct caacatggat
    15541 gagataaaac tgatcccgga attgtacgac gggatgcggg aggaggagtt cctgccattc
    15601 ataagaaagc agaacgacaa ggaccataca atcaccatat tcgggcacaa cccgatgata
    15661 actgaaatgg cagcatactt cgcctgcgac gagccccgct cgatagccaa aaccggcatc
    15721 tactgcctgg catttgatgt cgcaagctgg tctgaagtag agccggagag cggacgaacc
    15781 ctttactatc ttactccttc tgacctgtca tgaacaaagc atatattccc aaagagataa
    15841 gctggctgtc gttcaacgcc agggtgctgc aggaagccgc caacaacaat gtacctctcc
    15901 ttgagagatt caattttctc ggaatctatt ccaataacct cgacgagtat ttcagggtca
    15961 gggtggcaac tctcagacgc ctggcatttt tcagcaacaa ggccagaaac atcctgggct
    16021 actgcccgaa agctgccctg aagagtatcc aggaaacagt tctcagacag aatgaaatct
    16081 ttgagaagac ctattccgac ctgctcgatg aactaaagaa acaccaaatc cacttcgtga
    16141 atgaggaaca gctggatgac gcacagactg agttcgtgag gaactacttc cgcaaagagg
    16201 tccgttcgag gctcatgcct ctcattatca gtaaagacaa taagctgccc aatctcactg
    16261 atgatgccat ttaccttgcc atacaacttc gcaacagcga aaccggaaag aaaagatatg
    16321 ctttgctgga gatacccccg gctggcatac tgccgagatt tgtgatgctg cccgggaaaa
    16381 agggtatgag gtatgtcatc ttcctcgatg acgtaatcag gtgggggctg aaggagatct
    16441 tctccataat gccatatgat gagatcacgg catacaccat caaggtcaca agggatgccg
    16501 agcttgagat tgcggatgac atttcagaaa gctacataga caagatttcc aggagtctgc
    16561 agcaaagaaa aaaagggagc ccggtgcggt ttgtgcatga cagggagata cctgaagagt
    16621 tcctgaagat actctccaaa aagctgaacc ttggctctga agacgtgatc ataccgggca
    16681 acaggtacca taacttcaag gacttcatga agtttatcga gatcgaaggc gagggtctca
    16741 actacccgaa actgccaccg gtgaggcatc cggcacttca atacgggaag agcatcctct
    16801 cggttataat gaagagagat ataatgttct atttccctta ccatcctttt gatcatttca
    16861 ttgacctgct cagggaggca tcaatagacc cgtccgttgt atcgatacat atcacgctct
    16921 acaggctggc gaggaactca agtgtcatca atgcactgat gaatgccgcc cgcaacggta
    16981 agagcgtaac taccgtggtt gagctgcagg cgcggttcga tgaggaggct aatatccact
    17041 ggggcaacaa gttgctcgat gagggggtga aggtcattta tggcgtgccg ggactgaagg
    17101 ttcattccaa gctctgcctg ataaccaggg tcaagggcag gacaacgcag aggtatgcag
    17161 cagtgggcac cggcaacttc aatgaggata ccgccagggt gtacactgat cacctcctgc
    17221 tcactacaga gaagaagatc accaacgagg tgctgaaggt tttcaatttc ttcaatgcca
    17281 attaccgcaa ggacaaattc taccatttgc tggtgtcgcc cttcgggctg cgcaacaggc
    17341 ttatgattat gattgaaaac gagactaaaa atgctaagaa aggcaaagag gcttatatca
    17401 aaatcaagat caataacctg acggatgaag atattatctc gaggctttac aaggctgctg
    17461 atgcaggggt acagataagg cttatagtca gggggatgtt ctccatggcg ccgggagcaa
    17521 agggagttag tgagaatata caggccattg gtattgtgga caggttcctg gagcacacga
    17581 gattcatgat cttctgcaac ggaggcgatg aactggtcta tatcgcttcg gccgacataa
    17641 tgccgcgtaa ccttgaccgc aggatagaag tcacatgtcc tgtctgggac ccggcgataa
    17701 agaaggagat aaatgatatc ttcaacatcc agtggaatga taatgtcaaa gcgcgtatcc
    17761 ttgatgcgga gctgtcaaac acgatggtga gcaacggcga acctccgttg cggtcacaga
    17821 cagagataca ccggtatttt tcagggattg gttcgtagct tcttttnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnntgcc agtcaggtct tctccggttc cggattactg
    18001 tcaattctca gcctcaaacg gcagtgtcgg caggaactgt ggcgcccggc ggtattttgt
    18061 accctgttcg taggaataag caatctcaat aagaagcggc tcgctccatg ccctgccgaa
    18121 gaaggagatt ccaaccggaa ggccgtcaac ataacccatc ggcacggtga cattaggata
    18181 accggccacg gctgccggtg atgaactgct gacagtgtaa gagtcgccgt tgaccaggtc
    18241 ggtcttccag gccggtgagc ctgtcggggc aatgaacgca tcaaggttat actctttcat
    18301 taccttgtct attccctcat ctctcacgag ccggttcatc ttctccaatg catccaggta
    18361 ttccctggag gtgaggtcac ctttgttctg cgcttcaatc agcagttcct gatcataata
    18421 tcttaactca atgctgtcat ttctattgaa ctctatcagc tcctccacgc tctttaccgg
    18481 ggcattgtcg cccagggagg caaagtattt gttcagcccg tccttgaatt cgtagagcat
    18541 tacctgaaat gaaagggagc ccacctcgcg actatatatg ttatctatct ctatcacctc
    18601 tgctccctgg cttttgagaa agtcaaccgc atgctgcatc agggtgtcaa ccttgaagtg
    18661 tgttcccctg gccgatgtgt accagcccag tcgtttgccc tccagtcccc cttccttcag
    18721 aaaaggagta taatcagtaa gatatttgcc ctcagaagct gctgtcttgc tgtcgttcgg
    18781 atcaattccc gtcattgcac cgagggctgc agctgcgtca ctcactgtcc ggcccatggg
    18841 gcccggtgta tcctgggtga atgagatggg aattattccg ctgcggctaa gcagacccac
    18901 tgtaggtttg ataccaacga tgccgttggc ggttgagggg catactatgg agccattggt
    18961 ttcagtgcct atggatatca tgcagaggtt tgctgagacg gccacacctg atccggagct
    19021 tgatccgcac ggattgcggt caaggacata gggattcttt gtctgtccgc ccacgccgct
    19081 ccagccgctt gaggaaaggt tgccgcggaa gttggcccac tcgctgaggt tagccttgcc
    19141 tatgatgacg gctcctgcct cacgcagctt catggctata tagctgtcct gcagcgggcg
    19201 cgagccgctc agtgcgcgtg agcctgcagt ggttgacatc tggtcatggg tgtcgatgtt
    19261 gtctttaagc agcaccggca ctccatgcat gggaccgcgc ttcctgccct ccttcagctc
    19321 cgcgtcaagc tgtgctgcta tggccagtgc atcaggattg acctcaatca ctgcattgag
    19381 ggttggtccg ttgtcatcaa tggcgctgat ccggtcaagg taagcctgta ccacatcggt
    19441 aacggtgtat tcacccttgt catagccctg ctgaagctgc atgattgtgt attcctcgaa
    19501 acggaacgaa tttgctaatc ctgttccctt tttttctcct ctgctgcacg agaagaccgt
    19561 aattaatact aacaggaaaa ttttgggtag taaataattc ttcatgtccg atagcttgat
    19621 aattaaatgc atcacataaa gcaaagtaat aaaaaaatat aaaagtgaat gtttgtataa
    19681 tttctcatca agccaggagt aattattatt cgttgttttt actggtagtg ttccaattag
    19741 tgtgtctggg
//
LOCUS       DAOPBK010000023        19656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2882_length_19656_cov_5.386715, whole genome shotgun sequence.
ACCESSION   DAOPBK010000023 DAOPBK010000000
VERSION     DAOPBK010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19656
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2882_length_19656_cov_5.386715"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="PLA17_02430"
     CDS             complement(<1..2260)
                     /locus_tag="PLA17_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPK84301.1"
                     /translation="MKRMMLLTLMLPVALLVYPQDKKALTIDDLVTWNRISERVISDD
                     GSLIAFRTEPLKGDPVITLYDGTGSLKATFRCATGATVSSDSRFLFFTIKPPEEEVRA
                     LRLKKTKKDDMPLDKLGIYNVTTGVTDTVARLKSYKTPDRWSGWLAWQCEPLKEKPAA
                     MPDTAAGDNGKGKKPKSESADNGYTLYVRNLASGLTDTVRFVTDYLFAEEAEKLMYAT
                     TGEDGIMEPGVTIIDLKRGEKSMLYTGKAKYRQLAMDKKGERAAFIISFDEKDKAGNT
                     YSLCFWNGKGVASAVATKGTAGLPEGWIINENARLSFAEKSPRLFFGTSPEYKIRDTT
                     VLEDERAVVDVWHWSEGVLHTAQVVRKAREMKRTYTAVCHTDRGKVVQLATAEMPEVQ
                     LPEKGDGATALGISSLPYELESMWEGRSGNDLWTVDIMTGNAVKIKENLKARPRFSPG
                     GRYLYWYHGADSSWYSYDVTAGKETRLTTPATLRVWDELNDVPDIPGSYPAAGWLKDD
                     RALLVSDRYDIWSLDPAAATPARNVTSNGRQEGIRYRLLDFDPENDYIDPSERQYLIG
                     VNESTRAEGFYSLDIRKKNPPVRLAGGDYTLGIPVKARKADRLIYTKENFTLFPDYLL
                     TDLSFRNETRLTDANPQQKDYLWGTAEVVKWTSLDGRPLEGLLYKPENFDPAKKYPMI
                     VNFYEKSSTELNRHRIPEPGRSTIDYHYYTSNGYLIFNPDVYYIDGYPGQSACNCVMP
                     GIMSLIEKGFVDE"
     gene            2497..3480
                     /locus_tag="PLA17_02435"
     CDS             2497..3480
                     /locus_tag="PLA17_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84302.1"
                     /translation="MNRIVLLAGRLSVMILISFAALPASAQGTVVTGIITDHRNIPIV
                     GVRACQINSTNCTVSDINGIFHLVLDEGKEKILNVTCPGFNPAEAVISDTTTFPVRII
                     LVPMYIPEGSWANADDYLEPGGQTTIRSALSLSLIFSDFSEFNEQLGSYNTDAMEYFS
                     VTGPEMGASFSGFYAGLGVGFGYHYEDEHESLAVNLNNTMYKLITGYDIVNSTRIRMT
                     PALSVNWLKFRLKNSPNERRVPLEQYLSDREIDLRFNQVVAMAGLNLEYKFYSGINET
                     GNYWSVGIYGGYAVKVNRKPWIWSDGNRITTDNEIRLKPFTAGISVSFHGR"
     gene            3524..4510
                     /locus_tag="PLA17_02440"
     CDS             3524..4510
                     /locus_tag="PLA17_02440"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK84303.1"
                     /translation="MNYRKLGKTGFEVSEISLGTWQVGGKWGSKFDHTLAESIIREAI
                     DNGVNFIDTADIYSDGESEKAVGKTVRESGAAVRIATKCGRQINPHVNRGYTPEVLRG
                     FVEESLKRIGTDCIDLIQLHCPPTEVYYRPEIFGEFEKLKEEGKILNLGVSVEKIEEA
                     IKAIEYDNVTTVQLVYNIFRQRPREKFFSMAAEKNIGLIIRIPLASGLLTGKFGPDTR
                     FAPGDHRFFNRGGMFFDKGETFAGVPYDAGLRAVSRLKELFPESTRLAPVALNWILIN
                     GEVSCIIPGASSPGQVRSNLEALTFPGFTEEQKRAITAIYEEDIKPLVHHLW"
     gene            4736..6331
                     /locus_tag="PLA17_02445"
     CDS             4736..6331
                     /locus_tag="PLA17_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPK84304.1"
                     /translation="MFKKIPHTYVIVFSIVVLCAVLTWIVPGGAFERETVNVNGIDRS
                     VVIPGSFHYTEKNPQTWQVFSALFDGFVDKADIIVFILIIGGAFWIMNESKAIDVAIG
                     SVLRFTEKLGNRGVIRAIGADNLIFIVIMTMFSFFGAVFGMSEETIAFTIIFVPLAVS
                     MGYDSIVGVSLCFVAAALGFAGAFLNPFTIGIAQGLSDLPLFSGLEYRLVMWIIISLL
                     GFAYILVYARKIKREPEKSPVYDDDDYWRKLHSEKQLKIDYHTPLSAWIVYIILTVVM
                     ILFSVFYPVSSLSFGESQLRVPAIPVLTGVFVFTGWLTLRKSVHFFIIDLLLFTIFFL
                     ITGVMGYGWYIMEIATLFFAMGLASGIAMGYGANRITKLFLDGVKDIQSAALIVGLAG
                     GIIIILQNGNIIDTLLYKLSESISGAGKMASVSMMYITQTVINLFMPSGSAKAALTMP
                     MMSQFSDLIGLSRQATVVAFQLGDGFTNMITPTSGVLLGVLSVAKIPYEKWFKWVLPL
                     ILILFFLGFLLLIPTVFMELKGF"
     gene            complement(6487..7158)
                     /locus_tag="PLA17_02450"
     CDS             complement(6487..7158)
                     /locus_tag="PLA17_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK84305.1"
                     /translation="MKKVKFGLIIVLAGSLLLAGCASMNKATKGAVIGTAAGGAVGAV
                     VGKAAGNTAVGAIVGATVGGATGAIIGHKMDKQAAEIEEAVPDAKVERVGEGIVVEFS
                     SAVLFGFDQSDISTDAKTSLDKLVKVLNTYPDTNIEVQGHTDSKGSEAYNQTLSEKRA
                     GAVSAYLIAKEINAERITTKGFGETMPEYDNETEEGRAQNRRVEFLITANEKMIAEAE
                     AEAQK"
     gene            complement(7201..8742)
                     /locus_tag="PLA17_02455"
     CDS             complement(7201..8742)
                     /locus_tag="PLA17_02455"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPK84306.1"
                     /translation="MSRKDDFFRSINAGYACKGDFIVLGGAMLDGEAIADAHVKIPLK
                     TLNRHGLIAGATGTGKTKTLQVLAEQLSLKGIPSLVMDVKGDLSGLARPGQENDFIRN
                     RHGKINLPYSTMGFPVELLTISEQNGVRLRATVSEFGPVLFSRILDLNDIQGGIMSVI
                     FKYCDDNRLALIDLKDIRKVIQYLTGEGKDELEKEYGAMSTASTGVILRKIIELEQQG
                     AERFFGEESFDTSDLMRRDSGGNGYINIIRLTDIQDKPKLFSTFMLSLLAEVYNTMPE
                     KGDVPKPELVIFIDEAHLIFSQASKVLLEQIETIVKLIRSKGVGVYFITQNPMDVPAS
                     VLAQLGLKVQHALRAFTAKDRKDIKLTAENFPISDYYRTEEAITSLGTGEALVTALNE
                     KGVPTPLAVTMVRAPMSRMDILTPEEIDSINRSSQLVTKYSRVVDPESAAEMLEKKIR
                     DAEERRKEMETRRDYEKDYRRSTGRRTGTVTRRRTTTHPVVKVLTSATFIRGVMGILT
                     KAMKK"
     gene            complement(8818..9924)
                     /locus_tag="PLA17_02460"
     CDS             complement(8818..9924)
                     /locus_tag="PLA17_02460"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HPK84307.1"
                     /translation="MKKTIILLLVVAVAAACAREPKFTLTGTIDGAGNETVVLQKRIP
                     GGYQVLDSATIENGTFKIEGVIDYPQMVNLAIRGKRGGLNFFIENSDIAVHGHADSLY
                     MGSVSGSATQAEYDSYKALLDETNAEMTKLYDSYRQARTDGNEELVASLEKEIEALDE
                     KQMNIKKEFLANNTSSFIAPAVLSEISYYLEADEMEGILNSFDTTLNQVQTVITLKER
                     LVQLKAVAIGQKAPDFTLNDPDGNPISLSSKIGGDTKLLLLDFWASWCGPCRAENPFV
                     VKTWTEYNKKGFDVFGVSLDRSREDWLKGIQDDKLTWTHVSEVKYWDCAPAKLYAVSA
                     IPSNFLIDENGIIVGHNLRGDALAEKVKEILDAR"
     gene            complement(9987..11087)
                     /gene="meaB"
                     /locus_tag="PLA17_02465"
     CDS             complement(9987..11087)
                     /gene="meaB"
                     /locus_tag="PLA17_02465"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPK84308.1"
                     /translation="MSRERRSAGALSVQQGVPQPPSINPEMMNRLNKKRRKMLSADDY
                     VDGILRGNRTILSQAITLIESNLTEHYDLAQEVVERCLPTSSRSVRIGITGVPGAGKS
                     TFIETFGTMLTRHYGRRLAVLAIDPSSQQSGGSIMGDKTRMEQLSTDPDAFIRPSPSA
                     GTLGGVARKTRESIILCEAAGFDTIIVETVGVGQSETVAHSMVDFFLVLMLAGAGDEL
                     QGIKRGIMEMADTIVITKADGNNIQKAEMARVQYKNALHLFPPAPSKWAPRVMTCSSV
                     EKTGITDIWEAIRDYVSVTKSNGWFWERRREQAVTRMHETIIDSLKQSFYTNPDVTAM
                     LPELERKLREGEITSYKPAALLLDKYFGNTCK"
     gene            complement(11126..12202)
                     /locus_tag="PLA17_02470"
     CDS             complement(11126..12202)
                     /locus_tag="PLA17_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPK84309.1"
                     /translation="MKRNISFLILMLVCAGMMAQPKLNFANTRHNYGTIKEEAGKQEA
                     VFNFTNTGDSVLVLTKVQSSCGCTAADYTRSPVPPGGKGYVKAVFDPKGYSSRFAKSV
                     TVYSNAKPAVVVLVIEGNVAPREKTVEELYTFAVGPVRFQSNHLAFTTTTKSEKKIRV
                     MPVINTSKEPATVEFEGLPAHLQLKVSPATLKPGEKGMIEGTYDAKLNSSAWGNVNDL
                     VRLKVNGQSQPNIYLYVSANLVEDFSGLTAQQLANAPVFKLDMNTVDFGQIKQNSSAD
                     IEYFFTNEGKSDLIIRHVKAGCGCTALMPTDNVIKPGARGSIKAKFNSGGYKGQIYKN
                     IFVYTNDPKNSEVMLMIKGEVLVE"
     gene            12420..15695
                     /locus_tag="PLA17_02475"
     CDS             12420..15695
                     /locus_tag="PLA17_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84310.1"
                     /translation="MIRKGLHIPLLALFFLLAMAPASGQFYNGMQMNFGKNRVQHKNF
                     YWTYFRFDEIDCYYNEFGREVAEYACKVAYEKLNEIEDYFDYTVEKRPILIVYNKKND
                     FRQSNIGLISAEDDYNVGGYSRVIKNKIMLWFNGDHEEFSKLIAAAIAEVVVYEMLYN
                     ADLRDRTTSGNQIQIPDWYIKGLINYVAWGWDSETDNRVRDAFENNRYKNLDNLEYEE
                     AIFAGHSFWKYIGDTSGDAIIPNIIYLSKIYRNVEDGFLYGLGKSIKDTYRGWREYYS
                     NYYNTKPGENYQVPREIKKAKQYERFTDVKVSPDGRYVVWVANDWGKRRIWMHDVSTG
                     KSRKIFTAEPKYEQSVDHTYPVLTWHPGGQILTFANEEKGGVQLYFYRTAEKKFEQRT
                     MPFFEKVLSLSYSPDGSRLLVSAVINGMTDIFVHTIISGTNERITYDIADDLNPLFVK
                     GSDNVIVFSSNRLTDTLSNRGDPQERTGLTYNLFTYNLGTADGKLTRLGDDSMQDLTK
                     PVQPTSSSAGFIGNGNGIMNHYTGTIDSAIAYIDTALHYTYFITPRQTTDLPRNLEAF
                     DRSGGQDAGVIFNQGRYRIFTGPTSATQVADTADIKTTTWRMEELEMLRAADSINQMR
                     DWLLAERARMNDTLKKPLYEYYAPANEPIDFRNYVFEKEKENYYELQWRKDYMDINLD
                     TSRLKMPEALVYRPAFYNNYTATQIDFNFLNNTYQAYNGGAPYFNPGLNMLFKIGTLD
                     LFEDYRITGGFRFSGNFDSNEYLVSLEALRGRFDKQVIFHKQSFDSYNDTSYYKIKSY
                     TTYMSLRKPLSAVLALKGTLTYRYDNFVVQSTDQASLIAPSFNRHWAGLKGEVIYDNT
                     RKRTINIYYGTRFKVFGEYYREVTGRKSDMFVFGADIRHYLKIHRDLIWANRFAASTS
                     FGPSRLIYYMGGVDNWMKLPFGRLPQFDQTIPVNPNVRYGFQALATNMRGFTQNIRNG
                     SNFALINSEIRWPVVRYFAGHPLRSNFLNSLQLVGFYDVGMAWSGWDPWGNENYWNDE
                     VYRNGPVVVTVDAMREPLVMGFGGGARAQLFGYFIRADLAWGVDNGYLLPKIFYLSFS
                     LDF"
     gene            15875..16042
                     /locus_tag="PLA17_02480"
     CDS             15875..16042
                     /locus_tag="PLA17_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPK84311.1"
                     /translation="MAYKISEDCTACGTCIDECPVEAISEGDIYKIDPDICTECGACA
                     DVCPVEAIHPA"
     gene            complement(16294..16968)
                     /locus_tag="PLA17_02485"
     CDS             complement(16294..16968)
                     /locus_tag="PLA17_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84312.1"
                     /translation="MKYLIYIGLASILLISCEDNLQFEKMPCTYLDYYYYRDEPYYLG
                     EMSDEYILIACDQSNNDSSIRDFIKSIDFFDHSFNYEINEITNYPYKYLIAKLIKKCT
                     CEEIAWILDSLKQAPIVVYTHYTTKTNDCSNLIWEPIGKLCVNTYSNIFYVRVKDAGN
                     ISDLNNIISETNTTLIEQDRFMSNWFSLSAIKNSKGDALHMANYFYETGLFDACEPDI
                     IKIAIE"
     gene            complement(17145..18182)
                     /locus_tag="PLA17_02490"
     CDS             complement(17145..18182)
                     /locus_tag="PLA17_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84313.1"
                     /translation="MTHNARIIAAAALVLLTLFSSSCRRDPYRVNVSGIALDLRIRDL
                     GSEIFGTPPNEMAAKADSLRIEYGRVLDIYSTVIGLGDPSDERWNSAFVLFATDLRNL
                     DLWDTVKKVWPDTERLEKELESAFRHYKHYFPDRVVPQVVACLTIFNNSIIVDDSLLM
                     VSLDRYLGAGSAYYPAIGIYNYQARKMTPDYTASDCMYAWAATEWDYNSAGYGAKNLL
                     NTMLHEAKLVYFTRRMMPVLADTVLFGFTGRQLEFCKESEAMIWEYLVSKDLLFSTDG
                     FMIRKFTGEAPFTSYFTEQSPGRAVVWTGFRIIERYMNNNPDVTMEELMAMTDCQVIL
                     AGAKYNPKMSN"
     gene            18259..18957
                     /locus_tag="PLA17_02495"
     CDS             18259..18957
                     /locus_tag="PLA17_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPK84314.1"
                     /translation="MKRAIKRTVSGTMIFVLMAALPARAVAQELVFVVHADPVISWMG
                     SNESEYVSEGARAGFDIGLNVLHYFAENYAISSGISLISAGGRQSSTESHTLVFNNLT
                     PPTIPPGDEMKYNLRYLNIPIGVRLQTNPVGYLTYFTDIGFDVRLLIKSTIDLPTLQI
                     FAENAKNEVYGMNAGWHIGGGIEYELNFDASLIAGISYAQDFFDITKDLKDVNQHPDR
                     SGLRMFKIRLGLKF"
     gene            18968..>19656
                     /locus_tag="PLA17_02500"
     CDS             18968..>19656
                     /locus_tag="PLA17_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPK84315.1"
                     /translation="MKVALAQLSYIPGDIAYNSGKILTAIEEARRQGADLVVFSELAV
                     TGYPPLDLLRRSDIIDSSMAAIREIATHCNEIAAIVGGPSPNTGDYGKELHNSAFFLH
                     KGKIKAVVNKTLLPNYDIFDEERYFKAGDTFRPVELNGQKIAVTICEDIWYGQPFGDK
                     GPRRLYSMAPLDEIMKEHPSFIINIAANPFAHNRISVREEVFIRNTRKYMVPLISVNQ
                     TGGYSELIFDGS"
BASE COUNT         4947 a         5116 c         4598 g         4995 t
ORIGIN      
        1 tctcgtctac aaatcccttt tcaatgagtg acataattcc gggcattacg caattgcagg
       61 cactctgccc cggatatccg tcaatgtaat atacgtcggg attgaagatg agatacccgt
      121 tactcgtata gtaatgataa tcaattgtcg acctgccggg ctcgggaatc ctgtgccggt
      181 tgagctccgt agaactcttc tcatagaaat tgacaatcat gggatacttt ttcgccgggt
      241 caaagttctc cggtttgtaa aggagcccct ccagcggccg cccgtcaagc gaggtccact
      301 tcaccacttc cgccgttccc cacaggtaat ccttctgctg cggattggca tctgtcagcc
      361 ttgtctcatt cctgaacgaa aggtcagtga gcaggtagtc agggaaaaga gtgaagttct
      421 ccttcgtgta gatcaggcgg tcagccttcc tggctttgac cggaatgcca agggtataat
      481 ctccccctgc aagcctgaca ggcggattct ttttgcgaat gtcaagcgaa tagaagcctt
      541 ctgcccgggt agattcgttt acaccaatca gatactgcct ttccgaagga tcaatataat
      601 cattctcagg atcgaagtcg agaagccggt acctgatccc ctcctgcctt ccgttcgaag
      661 ttacattcct ggcaggggta gctgcagccg gatcaagtga ccagatatca tatctgtctg
      721 ataccagtaa agccctgtca tccttcagcc atccggctgc cgggtatgac ccgggaatat
      781 caggcacatc attcagctca tcccataccc tgagggttgc aggggttgtc agccttgtct
      841 ccttccctgc cgtgacatca tatgagtacc atgagctgtc agcaccatgg taccagtaga
      901 gatacctgcc gccgggcgaa aagcgtggcc gtgctttgag attctccttt atcttcaccg
      961 cattgccggt catgatatcc actgtccaca gatcgtttcc ggaccggccc tcccacatcg
     1021 attcaagctc atagggaagc gatgatattc ccagggctgt tgcaccatca cccttttccg
     1081 gaagctgcac ctccggcatc tctgccgtgg ccagctgaac caccttcccg cggtcagtat
     1141 gacagacggc tgtataggtt cttttcatct cgcgtgcctt cctaacaacc tgggcagtat
     1201 gaagaacacc ctctgaccag tgccatacgt ctacaactgc acgctcatcc tccagaacgg
     1261 tggtgtccct gattttgtac tcaggagatg tgccgaaaaa cagcctcggt gacttctccg
     1321 cgaagctcag cctggcattc tcgttgatga tccacccttc aggcaggcct gcggttccct
     1381 tcgtagccac agccgatgcc actcccttgc cgttccagaa acagagtgaa tatgtgtttc
     1441 cggctttgtc cttttcgtcg aacgagatta tgaatgcagc cctctcaccc tttttgtcca
     1501 ttgccagctg tctgtatttc gccttaccgg tgtaaagcat actcttctcc cctctcttca
     1561 ggtcaatgat tgtcactcca ggctccatta tgccatcctc accggtggtg gcatacatca
     1621 gtttctccgc ctcttcggcg aaaagatagt cggtgacgaa cctgaccgta tctgtcaggc
     1681 cgcttgcaag attcctgaca taaagggtgt aaccgttgtc agccgactca ctcttcggct
     1741 ttttgccctt tccgttgtca ccggcagccg tatcaggcat tgccgccggc ttctccttca
     1801 gcggctcaca ttgccatgcc agccaccctg accacctgtc aggggtcttg tatgacttca
     1861 gcctggcgac agtatcagta actcccgttg taacattgta tatgccaagc ttatccagcg
     1921 gcatatcatc ctttttcgtc tttttcagcc ggagtgccct cacctcctcc tctggcggtt
     1981 ttattgtaaa aaagagaaat ctcgaatcag atgagaccgt tgctccggtg gcacacctga
     2041 atgtcgcctt aagtgagccg gttccgtcat aaagggtgat caccggatct cccttcaggg
     2101 gctccgtcct gaaagcgatc agggagccgt catcggaaat aaccctctct gaaatacggt
     2161 tccaggtgac caggtcatca attgtcagcg cctttttgtc ctgtggatac accagcaacg
     2221 caaccggcag catcagggtg agcaacatca ttcttttcat actgcggata agataattgg
     2281 ttcaagactt aaaaataaca atttcctgat gcctgaaaag atgttttaca gccttccggc
     2341 aacagagccg gggttgcttt tatcccgtca attttaataa atttgagcgg catttctgaa
     2401 tcccgaacct gacttcagca acaaagagac aaaggctggc gtggaggtac agggaaggag
     2461 tccacacctg atcaaccatt tacaagacgt aaagctgtga acagaatagt tttgcttgcc
     2521 gggcggctct ccgtgatgat cctgatctca ttcgcggctc tgcctgcctc tgcgcaggga
     2581 accgtggtta ccggtataat aaccgaccac aggaatattc cgatagtggg agtcagagcc
     2641 tgccagatca acagcacaaa ctgcactgta tctgacatta acggcatatt tcacctcgtt
     2701 cttgatgaag gtaaggaaaa aatactcaat gtcacctgtc ccggtttcaa tccggcagag
     2761 gcggtgataa gtgacacaac aacctttccc gtaaggatca tcctcgttcc aatgtatatc
     2821 ccggagggga gctgggcaaa cgctgatgac tacctggaac caggggggca aacaaccatc
     2881 cgttcggcat tgagcctcag cctgattttc agcgactttt cagaattcaa cgagcaactg
     2941 ggaagttata acacagatgc aatggagtac ttctcggtaa ccggccctga aatgggagcc
     3001 tcattttccg gattctatgc aggattaggg gttggtttcg gataccacta cgaagatgaa
     3061 catgagtctc ttgccgttaa cctgaacaat accatgtaca agctgatcac aggctatgat
     3121 attgtaaact ccacgaggat aaggatgaca cctgcccttt cggtcaactg gctgaagttc
     3181 aggctgaaga attctcccaa cgaacggcgt gtaccccttg aacagtacct gtcagacagg
     3241 gagatagatc tcaggtttaa ccaggtagtt gcaatggccg gcctgaacct cgaatacaag
     3301 ttctacagcg gtattaatga aacgggtaat tattggtcag tgggcattta cggaggctat
     3361 gcggttaaag tgaacaggaa gccatggata tggtcagacg gtaaccgcat cacaacagac
     3421 aatgagataa ggctgaagcc attcactgca ggaatcagcg tgtcattcca cggcaggtaa
     3481 taaatgagaa aaccagccag tactggcaaa aatatacgga aagatgaact acaggaaatt
     3541 agggaaaaca ggatttgaag tttcggaaat ctctctcgga acatggcagg tgggtggcaa
     3601 atgggggtca aagttcgatc atacccttgc tgaatcaatt atccgtgaag caattgacaa
     3661 cggtgtcaat ttcatagata ctgcagacat ttactccgac ggcgagagtg aaaaagccgt
     3721 gggcaaaacc gtgcgtgaat cgggcgctgc ggtacgcata gccacgaaat gcggcagaca
     3781 gatcaacccg catgtaaaca gggggtacac tcccgaagtg ctccgcggct tcgtggagga
     3841 gtcactgaag aggatcggca cggattgcat cgacctgatc cagctccatt gtcccccgac
     3901 ggaagtatat taccgtccgg agatattcgg tgaatttgaa aagctgaagg aagaggggaa
     3961 gatcctcaac ctgggtgtca gcgtcgaaaa gatcgaggag gcaatcaagg caatcgaata
     4021 cgataatgtc accaccgtcc agttagtgta taacatcttc cgccagcggc cacgcgagaa
     4081 gttcttcagc atggctgccg aaaagaatat cgggctgatc atacgcatcc cgctggcaag
     4141 cggcctgctg accggaaaat tcggacctga cacccgtttc gcccccggtg atcaccgctt
     4201 cttcaaccgc ggcggtatgt tcttcgataa gggtgaaacc ttcgcgggag tgccttatga
     4261 tgccgggctc agggctgtgt caaggctgaa ggaactcttc ccggagagca cccggctggc
     4321 ccctgtcgcc ctgaactgga tactcataaa cggggaggtc agctgcatca tccccggagc
     4381 ttcctcaccc ggacaggtga gatcaaacct ggaagcactg acattccccg gctttacgga
     4441 agaacagaaa agagcaatca cggctatata tgaagaggat ataaagccgc tggtacacca
     4501 tctctggtaa ccggtaccgg caaaagcgtc ccggtccatc tctggtaacc ggtaccggca
     4561 gaagtgtccc ggtcctactc tggtaaccgg taccggcaga agcgccccgg tccggctccg
     4621 gtgggtgata accggctgcg ggattggttg cagcctgaag catgaattat atattccccg
     4681 cagaggataa agcaatgtaa aagaattatt attttgcact gccaaacatt ctctcttgtt
     4741 caagaaaatc ccccatacct acgtcattgt cttcagtatt gtggtgctgt gtgcagtgct
     4801 cacatggata gtgcccggcg gagcatttga gagggagacg gttaacgtca acggtatcga
     4861 tcgcagcgtg gtcataccgg gctcatttca ctataccgaa aagaacccgc agacatggca
     4921 ggtcttctca gccctgttcg acggatttgt tgacaaggct gacataattg tattcatact
     4981 catcatcggc ggtgcgttct ggatcatgaa tgagagcaag gccatagatg tggccatcgg
     5041 gtcagtgctg cgcttcacgg aaaagctggg gaacagggga gtgatcaggg ccataggtgc
     5101 tgacaacctg atcttcatag tgataatgac gatgttcagc ttctttggcg ctgtgttcgg
     5161 catgagcgag gagaccattg cattcacaat tatcttcgta ccgctggcag tctcaatggg
     5221 ttatgactcg atagtgggag tctccctttg cttcgttgct gccgcccttg gatttgccgg
     5281 tgccttcctt aaccccttca ccattggaat tgcgcagggt ttgtctgacc tgcctctctt
     5341 ttcaggactt gagtaccgcc tggtaatgtg gatcatcatc agcctcctcg gatttgccta
     5401 tatactggtt tatgcccgta aaataaagag agagccggag aaatcacctg tatatgacga
     5461 tgacgattac tggcgcaagc ttcattcaga gaaacagttg aaaatcgatt atcatacacc
     5521 tctgtcggcc tggattgttt atataatcct cacggtagta atgattctct tttcagtttt
     5581 ttatccggtc tcctcactct ctttcggaga gagccagctc agagtgccgg ccattccggt
     5641 cctgacagga gtgtttgttt ttaccggttg gctaactctc cggaaatcag ttcatttttt
     5701 cattattgac ctgctcctgt ttaccatctt ctttctgatt acaggggtaa tgggttacgg
     5761 gtggtacata atggagattg ccacactctt ctttgcaatg ggactggcgt caggaattgc
     5821 gatggggtat ggtgccaaca ggatcacgaa actgtttctc gacggggtga aggatattca
     5881 gtcagctgcg ctgatagtgg gtctcgcagg cgggatcatc attatcctcc agaacggcaa
     5941 cattattgac accctgctgt acaaactttc ggagtcaatc tccggtgcgg ggaaaatggc
     6001 atcggtaagc atgatgtaca tcacccagac ggtaatcaac ctctttatgc catccggctc
     6061 agccaaagcc gcccttacca tgccaatgat gtcacagttc tcagacctga tcggtctctc
     6121 aaggcaggcg acagtggtgg cctttcagct cggcgacgga ttcaccaaca tgataacacc
     6181 aacatcaggt gtgctcctcg gagtgctgag cgttgcaaaa atcccgtatg aaaaatggtt
     6241 caaatgggtt ctgcccctta ttctgatact ctttttcctc gggttcctgt tgcttattcc
     6301 cacagtattc atggagctga aagggttctg accgtgtccg gatctgtctg aaaatcagcc
     6361 acaataccac gattcataag ccataaggaa agagcccgac aacgggcaca agaggagcct
     6421 tctgcccctg atagaatcct tatcctgtaa tttatctggc tggcttataa aaactattac
     6481 ctgaaattac ttctgagctt ctgcttctgc ctcggcaatc atcttctcat tggcggtgat
     6541 caggaactca accctgcggt tctgtgccct gccctcttct gtttcattgt catattcagg
     6601 cattgtttca ccgaaaccct ttgtggtgat tcgctcagcg ttaatctctt tggctatcag
     6661 gtatgctgac actgcaccgg ctctcttctc ggagagggtc tggttgtatg cttcgctccc
     6721 cttgctgtca gtatgaccct gaacctcgat attggtatca ggataggtgt taagaacctt
     6781 aaccagtttg tcaagactgg tttttgcatc tgtagaaatg tctgactgat cgaaaccaaa
     6841 caaaacagcg ctgctgaatt caaccacaat accttcgcca acgcgttcaa ccttggcatc
     6901 aggaacggcc tcctcaatct cggcagcctg cttatccatt ttatggccaa ttatagcccc
     6961 cgtggcgccg ccaactgttg caccgacaat tgcaccaaca gcagtgttgc ctgcagcctt
     7021 gcctaccacg gcacccactg caccgccggc tgcagtgcca attacagcac cctttgtggc
     7081 cttgttcatt gaagcacatc ctgcaagaag aagtgatccg gccagaacaa taattaatcc
     7141 gaatttgacc tttttcattt cactcaattt aaattcaaca attataatat ctactcttcc
     7201 ttactttttc attgccttgg taagtatccc catcactccg cggatgaacg tggccgaggt
     7261 cagcaccttt accaccggat gggttgtggt cctcctcctg gttactgttc cggtcctcct
     7321 gcctgttgac cgtctgtaat ccttttcata atcacgccgc gtttccatct cctttcttct
     7381 ttcctccgca tccctgattt tcttctcaag catctccgca gcgctctcag gatcaaccac
     7441 ccgcgaatac ttcgtcacca gttgtgacga gcggttgatt gagtctatct cttccggggt
     7501 caggatatcc atcctgctca tcggggctcg taccattgtc accgccagcg gggtgggtac
     7561 acccttctcg ttcagcgccg tcaccagcgc ctccccggta cccagcgagg taatggcctc
     7621 ctccgtccgg tagtaatcgg aaatcgggaa gttttcagca gtaagcttga tatccttcct
     7681 gtctttggca gtgaaggcac gaagcgcatg ctgcaccttc aggccaagct gagccagcac
     7741 cgaagccggc acatccatcg ggttctgggt gatgaagtaa actcccacgc ccttggaacg
     7801 tataagtttg actattgttt cgatctgttc aagaagaacc ttgcttgcct gactgaaaat
     7861 gaggtgtgcc tcatcaatga aaatcaccag ttccggcttt ggaacgtcac ccttctcagg
     7921 catggtattg taaacctcgg caagcagcga aagcatgaat gtggagaaaa gcttcggctt
     7981 gtcctggatg tctgtcagcc ggatgatgtt aatgtatccg ttcccgccgg agtctctcct
     8041 catcaggtct gaagtatcga atgattcctc accgaagaac cgttcggcac cctgctgttc
     8101 cagctctatg atctttcgca ggatcacacc tgtggaggca gttgacatgg ccccgtattc
     8161 cttttcaagc tcatccttcc cctctccggt aaggtactga atcactttcc ggatatcctt
     8221 aaggtctatc aacgcaagcc ggttgtcatc acagtacttg aatatgacag acatgatacc
     8281 gccctgaatg tcgttcaggt caagtatgcg cgagaagagc acaggcccaa actcggagac
     8341 cgttgcacgt aacctgaccc cgttctgttc cgagattgtc agcagctcaa cagggaaacc
     8401 cattgtcgag tatgggaggt tgatcttccc atgcctgttt ctgatgaaat cattttcctg
     8461 ccctggtctg gccaggccgc tgaggtcacc cttcacgtcc ataacaagtg aaggaatacc
     8521 cttcagcgac agctgctcgg cgagcacctg aagcgtcttc gtttttcctg ttccggtggc
     8581 accggcaata agaccatggc ggttcagcgt cttcagcgga atctttacat gggcatcggc
     8641 tattgcctct ccgtcaagca tagcaccgcc aaggactata aaatcgccct tgcacgcgta
     8701 tccggcgtta atggacctga aaaaatcatc tttcctgctc attttctaag ggttaaagta
     8761 aataaaagcc gttgagacac ctgccccaac ggctctctaa attaataaaa aatcagatta
     8821 cctggcatca agtatctctt ttaccttttc tgcaagtgca tcaccgcgaa ggttatgtcc
     8881 tacgataata ccgttctcat caatcaggaa gtttgagggt atggcactta ctgcatacag
     8941 tttggcaggt gcgcaatccc agtatttcac ttcagaaaca tgtgtccagg tcagtttgtc
     9001 atcctggata cccttcagcc agtcttccct gctgcggtca agtgatacgc caaacacatc
     9061 aaatcccttc ttgttgtact cagtccatgt cttgacaaca aaggggttct ccgcgcggca
     9121 gggtccgcac catgatgccc agaaatcaag cagtaacagt ttggtgtcac ctccgatttt
     9181 ggagctgagt gagataggat tgccgtccgg atcattaagg gtgaaatcag gggctttctg
     9241 tcctatggca actgccttga gctgcacaag tctctccttc agtgtaatga ctgtctgaac
     9301 ctggttaagt gtcgtatcga agctgttaag aatcccttcc atctcatccg cttcaagata
     9361 atatgaaatc tcgctcagaa ccgcgggtgc aatgaatgaa gaggtattat ttgcaaggaa
     9421 ttccttcttt atattcatct gcttttcatc aagggcttcg atctcttttt ccagcgaagc
     9481 gacaagctcc tcatttccgt ccgttcttgc ctggcgatag ctgtcgtata actttgtcat
     9541 ctcggcattg gtttcgtcga gcagcgcctt gtatgaatca tattcggcct gggtggctga
     9601 accgctcact gaccccatat agagtgagtc agcatgtccg tgaacagcaa tatccgagtt
     9661 ttctataaag aaattgagac cgcccctctt gcccctgatg gccaggttaa ccatctgcgg
     9721 gtaatcaatc acaccttcaa tcttaaaggt gccattctca attgtagccg agtcaagaac
     9781 ctgataacca ccgggaattc ttttctgcag aactactgtt tcatttccgg caccgtcaat
     9841 ggtacctgta agagtgaatt ttggttccct ggcgcaggct gctgcaacag caaccaccag
     9901 caaaaggata attgtcttct tcatctgttg atttttaatg ggtaaacact ttcttctttc
     9961 gaaggttcaa agatacaaat aattgactac ttgcaagtgt tgccaaagta tttatccagc
    10021 agcaatgcgg caggtttata tgaggttatc tccccttcgc gaagttttct ttccagttcg
    10081 ggcagcatgg cagtcacatc aggatttgta tagaaagatt gcttaagtga gtcaatgatt
    10141 gtttcgtgca tccgtgtcac ggcctgttcc ctccttctct cccagaacca tccgttgctt
    10201 ttcgtaacac tgacataatc acgaatggct tcccagatat cggtaatacc ggttttctca
    10261 actgatgagc atgtcatcac cctgggagcc catttcgacg gtgcaggagg gaagaggtgc
    10321 agtgcgttct tgtattgcac cctcgccatc tcggctttct gaatattatt cccgtcggcc
    10381 ttggtgatga cgatggtatc tgccatctcc atgatgcccc gtttaattcc ctgaagttca
    10441 tcacctgccc cggcaagcat aagcaccaga aagaagtcta ccattgaatg agctactgtc
    10501 tctgactgtc ccacacccac cgtctccacg ataatggtat caaaaccggc agcttcacag
    10561 agaataatgc tttcgcgtgt cttgcgtgcc acgccgccca gcgtgccggc agaaggtgac
    10621 ggacgaatga aagcatccgg atcagtgctc agctgctcca tccgtgtctt gtctcccata
    10681 atgctcccac cgctttgctg gctgctcggg tcaatggcca gaacggcaag acgcctgcca
    10741 taatggcgcg tcagcattgt tccgaatgtt tctatgaatg tgcttttccc ggcaccggga
    10801 acacccgtaa tacctatcct gactgacctc gagctggtcg gcaggcaccg ttccacaacc
    10861 tcctgggcaa ggtcataatg ttccgtcaga ttactctcaa taagcgttat ggcctggctc
    10921 agaatagtgc ggttgccacg aagaatgccg tctacatagt catcggcact cagcatcttc
    10981 cgcctctttt tattcaggcg gttcatcatt tcaggattga tactcggagg ctgcggcact
    11041 ccctgctgta ccgagagagc cccggctgat ctcctttccc tgctcattgc gtctctgcct
    11101 ccatgctttc ctgtttagcc ttatcctatt ccaccagaac ctctcccttg atcatgagca
    11161 tcacctcgga gttcttcggg tcattggtat aaacaaagat attcttgtat atctgtccct
    11221 tatagcctcc cgagttgaat ttcgccttta tcgatcccct tgctcccggc ttaatgacat
    11281 tatcggtggg catgagcgcg gtgcacccgc aaccggcctt gacatgcctt attataagat
    11341 cactcttgcc ctcgttggta aagaagtact cgatatcagc cgacgagttc tgctttatct
    11401 gtccaaagtc cacagtgttc atatcaagct tgaagaccgg tgcattggcc agctgctggg
    11461 cagtgagccc tgagaaatcc tccaccagat tggcggaaac atacaggtat atgtttggct
    11521 gtgactggcc attgacctta agcctgacca gatcattcac attaccccat gcactgctgt
    11581 tgagctttgc gtcataggtg ccctcgatca tccctttctc tccgggtttc agcgtggcag
    11641 gcgagacctt cagctgaaga tgggccggca gcccttcaaa ctcaactgtc gccggctcct
    11701 ttgatgtatt gatgaccggc atcaccctga tcttcttctc tgacttggtg gtggtggtga
    11761 acgccaggtg atttgactgg aacctcaccg ggccaacggc aaaagtataa agctcttcaa
    11821 ccgtcttctc cctgggcgcc acattcccct caatcacaag cacgacaacg gccggctttg
    11881 cattcgaata tacggtaaca ctcttcgcaa aacggctgct gtaacccttc gggtcgaaca
    11941 cggcttttac atatcccttc cctccgggag gaacaggtga cctggtatag tcagctgccg
    12001 tacaaccaca tgacgactga accttcgtaa gaacaagcac cgagtctccg gtattggtaa
    12061 aattgaaaac agcttcctgc ttcccggcct cttcctttat ggttccgtaa ttatgacggg
    12121 tgttggcaaa attgagtttc ggctgcgcca tcatacccgc acaaaccagc atcaaaatca
    12181 gaaatgaaat attccgcttc atatgtactt atgtttttaa cctaatctgc ccgattcatc
    12241 atcaaatcaa tgctctcaac aaagatcagg ccagaagggt ttcaccataa taacaatcaa
    12301 aattaacaaa aaagtatgag ccattgtttt tttatttgtt tatagattag ttttgtcaca
    12361 cggatgcacc cgtttggaac agattttgct tcggatacgg tggcttgccg ccatttttta
    12421 tgatacgaaa aggactccac ataccgttac tggcactctt ctttctcctg gccatggcgc
    12481 cggcttcagg ccagttctat aacggtatgc agatgaactt cggcaagaac agggttcagc
    12541 acaagaactt ctactggacc tacttcaggt ttgacgagat tgactgctat tacaacgagt
    12601 tcggcaggga ggtggcggaa tatgcctgca aggtggctta tgaaaaactc aatgagattg
    12661 aggattattt tgactacacc gtcgaaaagc gacccatact catcgtctac aacaagaaga
    12721 atgacttcag acagagcaat ataggcctta tatccgccga ggatgactac aacgtcggcg
    12781 gctacagcag ggtgatcaag aacaaaataa tgctctggtt caacggtgac cacgaggagt
    12841 tcagcaaact tattgccgcg gctattgccg aagtggtggt ctatgaaatg ctctacaacg
    12901 ctgacctccg tgaccggact accagcggaa accagataca gatccctgac tggtatatca
    12961 aaggactgat taactacgtc gcctggggat gggacagcga gaccgacaat cgagtcaggg
    13021 acgcgtttga aaacaaccgc tacaaaaacc tcgacaatct tgaatacgag gaagccatat
    13081 tcgcaggcca ttcattctgg aaatacatag gtgacaccag tggtgacgcg attatcccca
    13141 acataatcta cctctcaaag atctaccgga acgtggagga cggattcctc tatggccttg
    13201 gcaagagcat caaggataca taccgcggct ggagggaata ttacagcaat tactataata
    13261 cgaagccggg tgagaactac caggtgccaa gggagataaa aaaggcaaaa caatatgaac
    13321 ggttcaccga tgtgaaggtg agccccgacg gccggtatgt tgtctgggta gccaatgact
    13381 ggggcaagag aaggatatgg atgcatgacg tatctaccgg gaagagcagg aaaatcttta
    13441 ctgcagagcc taaatacgag cagtcggttg accataccta ccctgtgctt acatggcacc
    13501 ccggcggtca gatactgaca ttcgccaacg aggagaaggg aggcgttcag ctttacttct
    13561 accgtactgc ggaaaagaaa tttgaacaac gaacaatgcc tttcttcgaa aaggtactct
    13621 cactctccta ctcccctgac ggatcacggc tccttgtatc ggcagtgatc aacggcatga
    13681 ccgacatctt cgtgcatacc atcatcagcg gcaccaacga acggataacc tatgacattg
    13741 ccgatgacct gaaccccctg ttcgtaaagg gaagtgacaa tgtaattgtc ttctcgtcca
    13801 acaggctcac agacacactc tccaaccgcg gtgacccgca ggagagaaca gggcttacat
    13861 ataatctttt tacatataac ctggggacag ctgacggcaa gctgaccagg cttggagatg
    13921 attcaatgca ggacctgacg aaacccgtgc agccgacatc ctcgtcagca ggcttcatag
    13981 gaaacggtaa cgggataatg aaccactaca ccggaaccat tgacagcgca attgcataca
    14041 ttgatactgc gcttcattat acctacttca tcaccccacg ccagacaacc gaccttccgc
    14101 gaaacctcga agcttttgac agaagcggag gacaggatgc cggtgtcata ttcaaccagg
    14161 gccggtacag gatctttacc ggaccaactt ccgccaccca ggtagctgac actgctgaca
    14221 taaagacaac cacctggcgc atggaggagc ttgaaatgct cagggctgct gacagcatca
    14281 accagatgag agactggctt ctcgccgaaa gggctcggat gaatgacacc cttaagaaac
    14341 ctctttacga atactatgcc cctgccaacg aaccgataga cttccggaac tacgtcttcg
    14401 aaaaggaaaa ggaaaactac tatgaactgc agtggcgtaa agattacatg gatatcaacc
    14461 ttgacacatc aaggctgaag atgcctgagg cacttgtcta ccgcccggca ttctacaata
    14521 actatacggc cacccagatc gacttcaact tcctgaacaa cacctatcag gcatataacg
    14581 gcggagctcc ttacttcaac ccgggactca acatgctctt taaaatagga acccttgatc
    14641 tcttcgagga ttaccgtata accggaggat tcaggttctc aggaaacttt gacagcaatg
    14701 aatacctggt cagccttgaa gcgctcagag gacggttcga caagcaggtc atcttccaca
    14761 aacagtcgtt tgacagttat aatgacacca gttactacaa aataaagagc tacaccacct
    14821 acatgtcgct caggaaaccg ctgagcgccg tattggcact gaaggggaca ctcacctacc
    14881 gttatgacaa ttttgtggta cagtcaactg accaggccag cctgattgcc ccttcgttca
    14941 atcgccactg ggccgggctg aagggcgagg tcatctatga caatacacgc aaacgcacca
    15001 tcaatatcta ttatggaacc aggttcaagg tcttcggtga atactaccgg gaggtgaccg
    15061 gaaggaagag tgacatgttc gtatttggtg ctgacataag gcattacctg aaaatccacc
    15121 gcgaccttat atgggcaaac cggtttgctg ccagcacctc gttcggtccc tcgaggctga
    15181 tctattacat gggaggcgtg gacaactgga tgaagcttcc gttcggccga ttgcctcagt
    15241 ttgaccagac aatacccgtc aacccaaatg taaggtatgg tttccaggcc ctggccacga
    15301 atatgagagg tttcacccag aatatccgta acggcagcaa ctttgcactg atcaacagtg
    15361 agatacgctg gccggtggtg aggtatttcg cggggcaccc gctgaggtca aattttctga
    15421 acagcctgca gctcgttggc ttctatgatg ttggcatggc gtggtcagga tgggatccct
    15481 ggggtaatga aaactactgg aatgacgagg tctaccggaa tggtccggtg gtggtaacgg
    15541 ttgatgccat gcgcgagccg ctggtcatgg gcttcggcgg tggtgccagg gcgcagctgt
    15601 tcggatattt cataagagca gacctggcct ggggcgttga caacggttac ctccttccga
    15661 agatcttcta tctctcgttc agtctcgact tctgacaccc cctcggcgga agctgttatt
    15721 acggcgtaaa ctgctccggt tcaggtattg catattgctg ccggaatgct gtttaacata
    15781 ctaccggaaa gggcaaattt tatttctctg tttcgttagg aaagaatgaa aataagagat
    15841 aattttacgc ccgtaatctt taaaaaaaag tataatggct tacaaaattt cagaagactg
    15901 cacagcttgc ggaacctgta tagatgaatg tccggttgag gctatatctg agggtgacat
    15961 ctacaagatt gacccggata tatgtaccga atgtggggcc tgcgccgacg tttgcccggt
    16021 agaggctatt catccggcat aaccgcattc cccattaagg aaaataaagt gggctgctcc
    16081 agatctaaag gggcagcctt tctttttgct tgcaatctgc tctccggaac caggcatctg
    16141 ccagcatgca tgatctacat ggcggacata tgtactcagg caagcgccca ccggaacagg
    16201 ctgtggcgcc tgccacaaaa ctttcctctt tagttgcccg attgccgcat catggagggc
    16261 caatgacata taccaacctg tttgaaaatt attttattca atagctattt tgataatatc
    16321 tggttcgcaa gcatcaaata aacctgtttc atagaaatag ttagccatgt gcaatgcatc
    16381 accctttgaa tttttaatgg cactcaatga aaaccagttg ctcataaatc tatcctgctc
    16441 aattaaggtg gtattggttt cagatattat gttattcaaa tcagagatat tacctgcatc
    16501 ttttactctt acatagaaga tattactgta ggtgttgacg cagagttttc caataggctc
    16561 ccagattaaa tttgagcagt catttgtttt ggttgtataa tgtgtgtaga caacaattgg
    16621 ggcctgtttt aaagaatcca gaatccaggc aatctcttca catgtacact ttttaatcag
    16681 tttagctata agatatttgt agggataatt agtgatctca tttatttcat aattaaaact
    16741 atggtcaaaa aagtcaattg attttatgaa atcccgaatt gaggagtcat tattgctttg
    16801 atcacaagca attaaaatgt actcatctga catttcaccc aggtaatagg gttcatcccg
    16861 gtagtaataa taatccagat aagtacaagg cattttttca aattgaaggt tgtcctcaca
    16921 acttatgaga aggattgatg ccaatcctat gtaaattaga tatttcatga ttgtgtcggt
    16981 atatttccac gcggcttcat tataaattaa tacgaaacca actgccgaaa cgctgcatga
    17041 aaaagagttt ttttcaacag accccgtcga agggcagata aacagactgc gacgaaagac
    17101 agaaaattgc taattgctaa ttgcacattg ttaattgcta attgctaatt gctcattttc
    17161 ggattatact ttgccccggc cagaatcacc tggcagtcag tcattgccat gagctcctcc
    17221 atggtcacat ccgggttatt gttcatatac ctctcaatga tcctgaatcc tgtccatacc
    17281 actgcccggc ccggcgactg ctccgtaaag taactggtaa acggagcctc accggtaaat
    17341 ttgcggatca taaaaccatc ggtagaaaac aacagatcct tgctcaccag atactcccat
    17401 atcatagctt cactctcctt acagaactcc agttgtctcc ccgtaaaccc gaaaagcacc
    17461 gtgtctgcaa gcacaggcat catccgtctg gtgaaataga ccagtttggc ctcatgaagc
    17521 atggtgttca gaaggttctt cgcaccgtaa ccggctgagt tgtaatccca ctccgtggcg
    17581 gcccatgcat acatgcagtc agaggcagtg tagtcaggcg tcatcttcct ggcctggtaa
    17641 ttatagatgc ctatcgccgg ataatatgct gagccggctc caagataccg gtcaaggctt
    17701 accatcagca gagagtcgtc aacaatgatg ctgttgttga atatcgttaa acatgcaaca
    17761 acctgcggca caaccctgtc aggaaagtag tgcttgtaat gcctgaacgc gctctcaagc
    17821 tccttctcaa gtcgttccgt gtcaggccac accttcttca ccgtatccca cagatcgagg
    17881 ttgcggagat cggtggcaaa gagaacgaaa gccgaattcc accgctcgtc tgaaggatca
    17941 cccagcccga tgactgtgct gtaaatatca agtaccctcc catactcaat cctcagggaa
    18001 tcagctttgg ctgccatctc gttcggcgga gtaccgaaaa tctcgctccc caggtctctt
    18061 atcctgagat caagagctat ccctgacacg ttaaccctgt aaggatcacg gcggcatgat
    18121 gatgaaaaca gggttagcag caccagggca gcggcggcga ttattctggc attatgggtc
    18181 atgcagtctg taatctaacg ttgaaagtac aaaataagga catttattta tatttttagc
    18241 ccggcaaaga gtgataagat gaaaagagca atcaaacgga cggtgtcagg gacaatgata
    18301 tttgtactga tggctgcgct acccgccaga gccgtcgcgc aggagcttgt gttcgtggtc
    18361 cacgctgacc cggtgatctc atggatgggc tcaaacgagt cagaatatgt cagtgaagga
    18421 gcacgggcag gattcgacat aggactcaac gtgctgcatt acttcgcaga gaactacgcc
    18481 atttcatcgg gcatcagcct catctccgcc ggaggaaggc agagctccac tgagagtcat
    18541 acactggtgt tcaataacct tacccccccg accatcccgc ccggcgatga aatgaagtat
    18601 aatctgaggt acctgaatat cccgattgga gtccggcttc agacaaaccc ggtgggatat
    18661 cttacctatt tcactgacat cgggttcgat gtcaggctgc tgatcaaatc tacgatagac
    18721 cttcccaccc tgcagatttt tgccgaaaat gccaaaaatg aagtctacgg aatgaatgcc
    18781 ggatggcata tcggaggcgg gatcgaatat gaacttaatt tcgacgcatc cctgattgca
    18841 ggcatctcct acgcacagga cttctttgac atcaccaagg acctgaagga tgtgaaccag
    18901 caccctgaca gatcgggact gaggatgttc aaaatcaggc tcggattaaa attctgacct
    18961 gccagctatg aaggttgctc tcgcacagct gagctatatc cccggtgaca tagcatacaa
    19021 cagcggtaag attctcacgg ccatcgaaga ggcacgccgg cagggcgctg acctggttgt
    19081 cttctccgaa ctggcggtga caggctaccc tccgcttgac ctgctgcggc gcagtgacat
    19141 cattgactcc tccatggccg caatccgtga gattgcaacc cactgcaacg agatcgccgc
    19201 cattgtgggc ggaccctcgc ccaacaccgg tgattacgga aaagaactcc acaactcggc
    19261 cttcttcctc cataaaggaa agattaaagc ggtggtgaac aagacattgc tgcccaacta
    19321 tgacatcttt gatgaagaga ggtatttcaa ggcaggagac acattccggc cggttgaact
    19381 caacggtcag aagattgcag tcaccatttg tgaagatatc tggtacgggc agccatttgg
    19441 cgacaagggc ccccggcgtc tgtacagtat ggcgccactg gatgagataa tgaaggagca
    19501 tccctcgttc atcataaaca ttgcagccaa tccatttgca cataaccgga tcagtgtcag
    19561 ggaggaggta ttcatccgca acaccaggaa atacatggtg ccgcttatct ccgtcaacca
    19621 gacaggaggg tattccgagc tcatctttga cggttc
//
LOCUS       DAOPBK010000024        19183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_2963_length_19183_cov_4.878816, whole genome shotgun sequence.
ACCESSION   DAOPBK010000024 DAOPBK010000000
VERSION     DAOPBK010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19183
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2963_length_19183_cov_4.878816"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLA17_02505"
     CDS             complement(<1..749)
                     /locus_tag="PLA17_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84316.1"
                     /translation="MKDLLFKKILLVPVLMTGVFALFLSSCDKDKDDDSDGKSLAVTV
                     NFSGTKVPSAGEQLMVVLVYSPLSEVDMENDGPDDILLHTLTANDITSGVTLTFTNID
                     PAKAEVYVAAFVDSDGDGSPGPGELLECYQDVSALDALTGKANATNVAGKKAITINLD
                     QILTMPSLAVTVNFTGTKPEVGKELMVVLVYSPFSEVDMENDGPDKLLSHFLTGNDIT
                     NGVTLTFEDINPFAEEVYVAAIVDFDGDGSPG"
     gene            complement(1156..1233)
                     /locus_tag="PLA17_02510"
     tRNA            complement(1156..1233)
                     /locus_tag="PLA17_02510"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1196..1198),aa:Val,seq:tac)
     gene            1544..7339
                     /locus_tag="PLA17_02515"
     CDS             1544..7339
                     /locus_tag="PLA17_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HPK84317.1"
                     /translation="MKQIHLSTLVLALLLALPPQSADAQVGPGGVGTLTNTQLWLRSD
                     SLIAIKPLYFVTSWIDLSGQMRDFSAVVMGQTVPTLSLNSINGYPAVTFSDQGGVGGD
                     FLGYNGSLGITGSDAATVVIVARNTTAADEQNGGLYMGQKNIGGTNAVRSYGLEYSDA
                     VRFNGQSQVFNDGHTPDDWRIIYYTNPAGASVSAYRAFLNGTPLTGNSTSDFVPSLVS
                     NFTLVGATQMNGTYNPAGYFNGDMTEIVVFSGQLNDAERVVLQNSLGAKYLLPIEDDH
                     YAWEATHSHDVSGIAAFNGTTFTNAWSTGMLSVTTPTDLSEGEYLFFGHDKAGARTWT
                     TTEVPAPGTYRLPREWRFDETSDVGTVTISIPASSLPALPSGYPTVGILIDDDGDFSS
                     GATMYRTNLSGGNYTVDLNIADGQYLTIIAFRPEVNFTVPAGSGPESDTPVTVQVSLN
                     YPHTSDVTVSYSATGGTATEGTDYTLDPGSVTIPAGSLTATFAINVIDDDVVEPSETI
                     ITTLSNPSTGVYIGTQNSYTYTILNDDTVYAAFSSATASGPEGNGPAPVSSPVIVVSG
                     GIITVPGSVIVTVSNGTASSDDWSQTGNMITIPAGDYTTPVAIPIPASVLTIQGDLTV
                     EPDETVNLNMNTFVTVAAGPVVSSVYTILNDDNSTASVTAPTAVIQEGGPGAAGTGTF
                     VFAFSNPVSNARTVSYTVTGTATSGTDYVALAGSFVMPAGALSYDLTLTSIADLLVEG
                     DETVRVTITGVSGTPAVDVDATPAEITIADDDLPVILFSPAAVSMTEGSTATIEVWLQ
                     NAPAGPVTINVSSTMTGLLNISPVSLTFNSSNFSTHQVITLQAIENVYLGDETDNVIL
                     AVDDALSFDPFDDLPDIIIPVTIINNDVASIVVNPTSVTVGENETATFTVVLSAAPPS
                     GNVVVDLVSNNTGVATIDIALLTFTAGDYNVPRIITVSGVNNFTVPDESTTISLVVND
                     ELSYDGYDGVTATVNVNVTNDDLAGFTVTPLTLTIDEGGPAGQFTIVLNAQPLTDVVF
                     DLVNADPVHITHVSQVTFTPENWNVPVVVMVTAIEDLLDADRTDVIAVTINQALSDNS
                     FDALPAQDVTVHIRDNDPPVITGCPDNITVNTSPGECSATVSWTPPVSTAPMTSTHLP
                     GSAFASGVTTVTYTSTDADGMVSTCSFTVTVNDTESPVISCKNITADLDASGNATIVP
                     SDVLLSAPTDNCAIADVTLSRSSFTCEDLGQVTVTVTATDASGNVALCEAVVTVADPF
                     VTSLNAGPDAEICTTEPSYIITGASATNMTLSWSTSGSGSFSDPAALNPVYTRGTTDL
                     TEVVLTLTGSKINGCGETLTDAMTLTFAGEPSANAGADKDLCSGTADVALSDATASNG
                     IISWSTSGNGTFSDPLIATPVYTFGSSDTGPVTLTMTVTSALCGTVSDNLTITFTPEP
                     VADAGPGGSVCRNEAGFQVAGASHAGGTVTWSSSGDGTFSDNTADNPYYTFGEADFAA
                     GSATLTMTVTGGGACGTETSSATVTINALPVPEIASQQQITCNGLTDGAVQLAASGGL
                     EPYAYSIDGGAFQASGDFTGLAAGIHVFEVMDDNGCVAGITVEITEPLPFTAVIDGTD
                     DVTCHGSSDGAIYATLSGGTEPYNISWAGPDGYTASTATITGLGAGTYTLTVSDRNNC
                     VTLSYIEVITQPDAIEITSVVISDNNGYGVTCPGSADGSITVAVQGGTPPLVLEWKGP
                     DGFTSDQPSISMLGAGEYRLTISDAAGCTLTADYTLTAPEELDISAITTDASCPDTPD
                     GSIDLTVSGGSGTLIYLWNDGATVADRTALLPGDYTVDVTDASGCTTTFKVTVGVMGY
                     DCLRIYEIITPNGDGRNDTWQLRNAWLYPDAEVFVYTRWGKLVYHSRNATDEWDGTFN
                     GKLLPNDSYHYVIHLNDGSEPRTGIISIISK"
     gene            7360..8349
                     /locus_tag="PLA17_02520"
     CDS             7360..8349
                     /locus_tag="PLA17_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPK84318.1"
                     /translation="MKTLRIVAVVGLLAVIPVTRAQQFPMYSQYMMNGFLLNPSYAGS
                     DYYTTFGLTVREQWLNLPHAPSTYAAAFQTRLLNDSYITKSTAVRKKISKPTKGGRVG
                     VGGYLFSDHNGIMHRTGVQLAYAYHLPVGDEQQLSFGLSLSAYQYFVDIGGAVMPDDV
                     QDELLNNYDQVVYIPDANFGVSYTTRRYYAGFAMTNLFRGTLMLGNGSENSRSELGHF
                     FLTGGMRFFPGTDWIIEPSVMLKSSDMVFKSFQVDLTGRVYYKDDYWLGLSYRTGDAV
                     ILMAGVKVDRFYLGYAFDFTLSEIRSYTYGTHELTLLARFGDNPRRYRWINKY"
     gene            8349..9767
                     /locus_tag="PLA17_02525"
     CDS             8349..9767
                     /locus_tag="PLA17_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84319.1"
                     /translation="MRHIIPFLLIVAACLSCDPLANSVSGRLAFSSDTISFDTLFSGY
                     GSATLELRIKNLEDTPVLIDRIWLGGGSGSPFRINIDGQPVAEAQNITLARGDSIFIF
                     IIVTIDPSGSDLPLAVTDSVNFLSGSHSGRVILEAWGQDVRLVTEDITSPAVWSEGKP
                     YVITGHLLVDTLVTLTLEPGTRVYMHHGAEVTVAGTLVAAGTPEKRITIATDRLEKEY
                     RDVPGRWKGVKFQSCSRGNILNYTDIRNAQIAVEIEGHPASVPDLTMNSTTLMHNSVA
                     SLAASNADLFAVNSLFAHSGFSTVSLSEGGNYEFIHCTISNIWEYSFRYEPALLISAG
                     DGTMPAVSVINSVISGSLPGELLIDAPAAEVESWFRADSSLIKVDTLTADWYSTPLFR
                     NVITTQSPRFIDENSWDFRPDTLSPLLDRAGKSEMKLWPADIRNKQRPAFDGPDIGAF
                     ERQEGEKRKEKETGMAGMHVRH"
     gene            9834..10823
                     /locus_tag="PLA17_02530"
     CDS             9834..10823
                     /locus_tag="PLA17_02530"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPK84320.1"
                     /translation="MRVILSVMAFLAMMLLQASGQDFSVMFYNVENLFDTVDDTTKND
                     EEFLPGGSRRWTASRYHRKLSAVARAVAVAGEWELPSLAGLCEVENEEVLRDLVYGTI
                     LSAGNYGIVHRESPDPRGIDLALLYRRDCFSFVEADAWLPADVDSMPVLTRNILHVKL
                     TAGADTLHIVICHLPSRKGGVLAAADLRERMVRLAGAGIDSIMSASGGRAAVIVMGDF
                     NTAPGEPLMEQFAREAGLVNLSTETAGEGKGSYRYQGTWEMIDQILVSTPMTDSTNVF
                     YALPGTFRVVDAPFLLTTDGTYPGKKPFPTYGGYSYSGGYSDHLPVTVRVSVR"
     gene            complement(10827..12239)
                     /locus_tag="PLA17_02535"
     CDS             complement(10827..12239)
                     /locus_tag="PLA17_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK84321.1"
                     /translation="MKIFPDDRIFEVVAGVADRENVEAYVIGGFVRDHIMGRVNPERD
                     IDIVVLGDGPAMARAVAREISRDIKVTVFRTFGTATFRYRNSDIEFVGARKESYSSDS
                     RKPSVSPGTLEDDQKRRDFTINALAISLNRDNPGEVLDPFGGIDDIRNGIIRTPLDPD
                     ATFSDDPLRMMRAVRFAAQLDFRIADETLTSIRSNAERIKIVSAERITTELNKIMTTA
                     KPSAGFLLLEQTGLLELILPEIARLRGVEDKDGKGHKDNFHHTLKVLDNLAEKSSDLW
                     LRWAALLHDVAKPVTKKFVQGTGWTFHGHEFIGAKMVPEIFKRLKLPLNDRMKYVQKL
                     VGLHLRPVSLVQDEVTDSAVRRLLFEAGEDIDDLMLLCEADITSGNRMKVLKHRENFA
                     LVRRKLKEIDEKDAIRTFQPPVDGEEIIATFNIPPGPMVGVIKSAIKEAILDGIIPND
                     HEAAREFMIRKGREMGLEPV"
     gene            12400..12768
                     /gene="trxA"
                     /locus_tag="PLA17_02540"
                     /pseudo
     CDS             12400..12768
                     /gene="trxA"
                     /locus_tag="PLA17_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            complement(12961..13158)
                     /gene="xseB"
                     /locus_tag="PLA17_02545"
     CDS             complement(12961..13158)
                     /gene="xseB"
                     /locus_tag="PLA17_02545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPK84322.1"
                     /translation="MAKKEIGFNEAMKGIEQILRNIEEGEPDIDRLSENVRKAAELIK
                     ICRRRLRETGEKIDGIMEELR"
     gene            complement(13158..14414)
                     /gene="xseA"
                     /locus_tag="PLA17_02550"
     CDS             complement(13158..14414)
                     /gene="xseA"
                     /locus_tag="PLA17_02550"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPK84323.1"
                     /translation="MNRDAKISLTELLGLVRDKIYEALPEAFWVVAEIAEMKVNSTGH
                     CYLELTGSDTPGGRVTSRARATIWASKYRSLNTLFNATAGIPLRAGITILFRATVEYH
                     ELYGLSLNITDIDPAYTAGDMALRREAVIRRLTAEGVLSMNSGLPLTLYPRNIAVVSS
                     SRAAGYQDFTDHLLNNPYDYVFKTTLFEAVMQGEETEASVIGALDRISEETGKFDAVA
                     IIRGGGSTIDLSWFDNYAIAFHVTQFPLPVLTGIGHEKDQSVTDMVAWKALKTPTAVA
                     GFLIDRTLECENRIISMAGEMASAVTGALEAADEQLSSFQNRTAATARLVVRVNNERL
                     GYHTETLRRSARNALQKAGDKTGRLEQSLRYLDPASVLKRGFTLTSRNSIIIRSAAEL
                     EEGDNITTHFEKGTVKSTVKEIKNKK"
     gene            complement(14411..16060)
                     /locus_tag="PLA17_02555"
     CDS             complement(14411..16060)
                     /locus_tag="PLA17_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPK84324.1"
                     /translation="MNRVVSALIPLLFVIAGIMPGKAQQRYPGYDELWFYRVWFSEKT
                     NSISEYSPEELLSPAAIARREKYGIPLVTESDIPVSSQHIRTLTGAGFRLRCTSKWLN
                     TALFTTTGAIKSDIAGEYPFIDSIQLVKHPVDPVKRNYSKYGITEPGMITDKFDPRLP
                     LNGDMLHKSGLTGLNITIAVLDAGFINADKIEALEPLRKRGGIITTRDFINSSSYVYD
                     YHTHGTSVLSILAGAMPGIINGTAPGAKYMLLRTEDVLSEFPVEEDYWIAAAEYADSA
                     GADIITSSLGYFEFDDPSMDYPFSDMDGDRPFITRGADMASSKGILVVNSAGNERNKA
                     WLRIIAPSDGDSVLCVGAVNHDLTISDFSSAGYSSDGRVKPDMVAPGVLIPLQFEPGV
                     WHSGSGTSFSCPVISGLSASLMQAVPDASPLEIINALRKSSDRYTRPDSLYGYGLPDF
                     LKTLRLLEDEHTFRPEAMMSAGPNPFTDEILVWFHEPPGSLTVTITGTNGTTILARHF
                     PAFAARLFRLDGLGSLAEGFYLVRIVTDQGEEVYKMIRTDR"
     gene            complement(16057..16833)
                     /locus_tag="PLA17_02560"
     CDS             complement(16057..16833)
                     /locus_tag="PLA17_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84325.1"
                     /translation="MKTRLIFILPLLLLSTTVMSQNVTDAGGKKQGPWVKKYPNGNIM
                     YQGTFRDDKPVGLFKRYTEEGLLLSELTYSDSKDEASAVFYHPDGLKAAEGKYVARSK
                     EGLWKFWSATRPHYLICEEYYHNDIRHGLSQKFYPDSTLAEKVAWDMGNRTGEWLQYY
                     PDGAICLRAEFVAGRLEGPFSYYHPNGNLQYEGHYKEDNRTGDWMVFKEDGSLKQIIT
                     YREGVPADPKLADQETKFLDDLEKNKGKIEVTDITGSVIK"
     gene            complement(17137..19059)
                     /gene="dnaK"
                     /locus_tag="PLA17_02565"
     CDS             complement(17137..19059)
                     /gene="dnaK"
                     /locus_tag="PLA17_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPK84326.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIPNSEGKRTTPSVVAFIE
                     NGERKVGDPAKRQAITNPRKTVSSIKRFMGRSFDEVSNEMKKMTYEVVRGDNNTARVK
                     IDDRNYSPQEISAIVLQKMKKTAEDYLGQEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LKVKRIINEPTAASLAYGLDKKAQDLKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDHLIIDWLADDFQKEEGIDLRKDPMALQRLKEASEKAKIELSSTTTTEIN
                     LPYIMPVDGIPKHLVKTLTRAQFEKLTDKLINAVIGPCQKALDDAGMKTSDIDEVLLV
                     GGSTRIPAIQQIVEKFFGKTPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETMGGVMTRLIESNTTIPTRKTETFTTASDSQPSVEIHVLQGERPMAAGNKTIGR
                     FHLDGIPPAPRGVPQIEVTFDIDANGILNVSAKDKGTGREQRIRIEASSGLKEEEIRR
                     MRDEAKANEAADKAAREIADKVNEADSMIFQTEKQIKEYGDKIPSDKKGRIENALGQL
                     REAHKNKDITAIDRALAEINAAWQAASEDIYKQTQGGPGPQAGPEAGPQAGTGGTTGG
                     KDGDEQVTDVDFEEVK"
BASE COUNT         4542 a         4837 c         4778 g         5026 t
ORIGIN      
        1 cccggacttc catcaccgtc aaaatccaca atagcagcga catatacttc ttctgcaaat
       61 ggattgatat cttcaaaggt aagtgttacc ccgtttgtaa tatcattgcc cgtaaggaaa
      121 tgagacagta atttatccgg accgtcattt tccatgtcaa cttcagaaaa gggagaatat
      181 actagcacca ccatcagctc tttaccaact tctggtttag tgcccgtaaa gttcactgtc
      241 actgccagtg agggcatcgt cagaatttga tccaggttaa tggttatcgc ttttttcccg
      301 gccacattcg ttgcatttgc ttttccggtc agagcatcca gggcactcac atcctgatag
      361 cattcaagaa gttcgccagg tcccggactt ccatcaccgt cagaatctac aaaagcagca
      421 acatatactt ctgctttagc cggatcgatg tttgtaaagg taagcgttac accgcttgta
      481 atatcattgg ccgtaagggt atgcaacagt atatcatccg ggccgtcatt ttccatgtca
      541 acttcggaca agggagaata tacaagcacc accatcagct gctcaccggc tgaaggaact
      601 ttagtacccg aaaaattcac tgtcactgcc agtgatttgc catcgctatc atcatctttg
      661 tctttgtcac aactgctgag gaataatgca aacacacctg tcattagaac aggaaccagc
      721 agaattttct tgaataataa atccttcata attactgtgt ttaaatgaaa cataggttta
      781 ataaaacata caaaaaacgt catctcaatt atgccttgtt atgccgaggg gcgcctaagc
      841 acctgtgttt caatatgaaa gacacgtcgc ttcattcaac atgatacttg atctataagg
      901 ataacgtaat gatttaaaat atgttacctt tttttgacaa aaactgaggc ggggagcctt
      961 tctattactc tctatgcagt aatcatttaa tgtgtcaaat acattataat caaatgtaga
     1021 tggaagtgcg ggtagatgca gcgagacact tcaataatca ggtataccgc tctttttata
     1081 cgagatatgt tgcaacaata atgcaaccgt ataaaacaat aaggaggtta acgctatgct
     1141 aacctcctca ttcactggtg ggcgatgtag ggatcgaacc tacgacctcc tgcttgtaag
     1201 gcaggcgctc tgaaccaact gagctaatcg cccggaaaga gagtgcaaat atacgctcta
     1261 ttttttattc cgcaatagcg gttttaaatt aaaaaccgac ttccgggaaa ggcaaaaccg
     1321 tttttgtaac tgccacacaa caacattgtg aatcatttta ttatgactgt taattatacg
     1381 ctttgccctg atagttccgt tattaaagaa tatatttata ttagcttttt ggtcatttat
     1441 gaccttgatt gagctgcgat tgtaaggtga taacctgagg gttgtagact caatagtttg
     1501 ataaatagtg tattatgatg cctaaacgct gatcctgact gtcatgaaac agatacacct
     1561 gtcaaccctg gtgcttgcac ttcttctcgc tctgcctccg caatcagctg atgcgcaggt
     1621 tggcccgggc ggagtaggca cactcaccaa tacgcaactc tggctaaggt cagacagcct
     1681 cattgcgata aagccactct attttgtaac cagctggatt gatctctccg gccagatgcg
     1741 tgacttcagt gcagtggtca tggggcagac cgttcctacc ctttcactga acagcataaa
     1801 cggttatccg gctgtcacat tctccgatca gggcggagtg ggaggcgact tcctcggata
     1861 taacggatca ctgggcataa caggatcaga tgctgcaacg gtggtgatag ttgcccgcaa
     1921 taccactgct gccgatgagc agaacggagg cctctacatg gggcagaaga atataggtgg
     1981 cacaaatgca gtcaggagct acggacttga gtatagtgat gctgtcagat tcaacggcca
     2041 gagccaggtc tttaatgacg gccatacgcc agatgactgg agaataatat actacacaaa
     2101 ccctgcaggg gcgtctgtct ctgcctatag ggcatttctc aacggtaccc cgctgacagg
     2161 taactcaacc tccgactttg tgccttcact tgtttccaac tttacccttg ttggagcaac
     2221 ccagatgaac ggaacctata acccggccgg ttatttcaac ggtgacatga cagagatagt
     2281 cgtcttctca ggccagctga acgatgctga acgggttgtg cttcagaaca gccttggcgc
     2341 aaaatacctg cttccgatag aagatgacca ttatgcctgg gaagcaaccc attctcatga
     2401 tgtgtcaggc attgccgcat ttaacggcac aaccttcacc aatgcatggt caacaggtat
     2461 gctttcagta actactccta ccgacctcag tgaaggcgag tacctctttt tcggacatga
     2521 caaggcaggg gccaggacat ggacaaccac agaggtgcct gctcccggca cttaccggct
     2581 gccaagggaa tggcggtttg acgagacttc agatgtcgga acggtgacaa tcagtatccc
     2641 ggcatcatca ctgccggcac tgccatcagg ctacccgacg gttggaatac ttattgatga
     2701 tgacggcgat ttcagttccg gagccacaat gtatcgaaca aacctgtcgg gaggcaacta
     2761 cactgttgac ctgaacatag ctgacggcca gtacctgaca ataattgcat tccggccgga
     2821 ggtgaatttt accgtaccgg caggatcagg gccagagagt gatactccgg tcactgtgca
     2881 ggtttccctc aactatcctc acacctctga tgtcacagtc agctactctg caaccggcgg
     2941 aacggcaaca gaaggaactg actatacgct tgatcccggt tccgtgacaa ttccggcagg
     3001 gtcactgact gccacttttg caatcaatgt catagatgat gacgtggttg agccttcaga
     3061 aaccataatt accactctct ccaatccttc cacgggggta tatattggta ctcagaacag
     3121 ttatacctat actatcctta acgatgacac cgtctacgct gcattctcct cagccactgc
     3181 ttctggtcct gagggtaatg ggcctgcacc ggtatcatca cctgtaatag tggtcagcgg
     3241 aggaattatc accgttccgg gaagcgttat tgtgacagtt tcaaatggca ctgcctcatc
     3301 ggacgactgg tctcagaccg gcaacatgat taccatacct gcaggcgact atacaacgcc
     3361 ggttgcaata ccaataccag catctgtact gactattcag ggagatctca cggtggaacc
     3421 tgatgagacc gttaacctga acatgaatac ttttgtgact gttgccgcag gtcctgttgt
     3481 gagctctgtt tacaccatcc tcaatgatga caactcaaca gcaagcgtca ctgccccgac
     3541 agcggtcatt caggaaggcg gaccaggtgc agcaggaaca ggcacctttg tatttgcttt
     3601 cagcaatcct gtttcaaatg caaggacagt atcatacact gtaacaggaa cagcaacctc
     3661 cggcactgac tatgttgcac ttgcgggatc atttgtgatg ccggcaggag cactgtcata
     3721 tgatcttaca ctgacttcca ttgccgatct cctcgttgag ggtgatgaaa cggtaagggt
     3781 caccatcacc ggagtctcag gaactccggc agttgacgtt gacgcaacac cagctgagat
     3841 tacaatcgct gatgatgacc ttcctgtcat tctgttctcc ccggcagcag taagcatgac
     3901 tgaagggtca actgctacca tcgaggtctg gctgcaaaat gcacccgcag gcccggtaac
     3961 catcaacgtt tcttccacga tgacagggct gttgaatatc agtccggttt cactcacatt
     4021 caattcatcg aatttttcca cgcatcaggt gataaccctg caggcaattg agaatgtata
     4081 cctgggcgat gagactgaca atgtaatact ggcagtggat gatgctcttt cgtttgatcc
     4141 ctttgatgac ttgcctgata ttattattcc ggtcaccatt atcaacaatg atgtggcctc
     4201 tatagtggtt aatcccacaa gtgtcacggt tggtgaaaac gaaaccgcga ccttcactgt
     4261 ggttctgtca gcagcgcctc cgtccggcaa cgttgtggtt gatctggtca gcaacaacac
     4321 cggggtggct accatcgata ttgccctgct cacctttacg gcaggagatt ataatgttcc
     4381 caggatcatc accgtatccg gcgtgaacaa cttcacggtg ccggatgaat caacaaccat
     4441 ctcccttgtc gtgaatgatg aattgtcata tgacgggtat gacggagtga ccgccaccgt
     4501 caatgtcaac gtcacaaatg atgatctggc agggttcacg gtcacgcctc ttacgctgac
     4561 cattgacgag ggaggtcctg ccggccagtt taccattgtg ctgaatgccc agccactgac
     4621 tgacgtggtt tttgacctcg tcaatgctga tccggttcat atcacccatg tttcgcaggt
     4681 gacctttaca ccggagaact ggaatgtgcc ggttgtggtc atggtcacag ccatcgagga
     4741 tcttctggat gctgaccgta ctgatgtcat tgctgtcaca atcaatcagg ccctgtctga
     4801 caacagtttc gatgcattgc cggcacagga tgtcacagta catatcaggg acaatgatcc
     4861 gcctgttatt accggttgtc ctgacaatat tacggtcaat acatccccgg gcgaatgttc
     4921 agccacagtc agctggactc ctcccgtgtc aacagcaccg atgacatcca cccaccttcc
     4981 gggttcggcc tttgcttcag gtgtgaccac ggtgacctat acgtcaaccg atgctgacgg
     5041 tatggtttct acctgcagtt tcacggtaac cgtgaatgat accgaatcgc ctgttatcag
     5101 ttgcaaaaac ataacggcag atctcgatgc ttccggtaat gcaaccattg tcccgtcaga
     5161 tgttctgttg tctgctccca cggacaactg cgcaattgcc gatgtcacgc tcagccgctc
     5221 ctccttcacc tgtgaagacc tgggtcaggt taccgttacg gtaacggcaa ctgacgcaag
     5281 cggtaatgtg gcactgtgcg aggcagtcgt aaccgttgca gatccgtttg tcacatcgct
     5341 gaatgccggt cctgatgcgg aaatatgcac tacggaacca tcatatatca tcacaggtgc
     5401 atcagctacc aatatgaccc tgtcatggag cacatcgggc agcggtagct tcagtgaccc
     5461 ggcagcactc aatccggttt ataccagggg tacaactgat cttacagagg tggttctgac
     5521 cctgaccggg tcaaagataa atgggtgcgg ggagacactg actgatgcca tgacgctgac
     5581 ttttgccggt gagccgtcag ccaatgccgg agccgacaag gacctctgca gcggaaccgc
     5641 cgatgttgcc ctgtcagatg caacggcatc caacgggata atatcctgga gtacatcggg
     5701 caacggcacc ttcagtgatc cgttaattgc cactccggtt tacaccttcg gaagctcaga
     5761 tacaggtcct gtcactctta ccatgacggt cacaagcgca ttatgcggga ccgtgagtga
     5821 caatcttaca ataaccttta cccctgaacc tgttgcagat gccggtccgg gaggatcagt
     5881 atgccggaac gaggccgggt tccaggtagc cggcgcttcc catgcaggag gcacagtaac
     5941 atggagcagc agcggggacg gcactttcag tgacaatacg gcagataatc cctattatac
     6001 tttcggcgaa gcagactttg cagcaggatc agcaacactc acaatgacag ttacaggtgg
     6061 tggagcctgt ggcacggaaa cctcttcagc cactgttaca atcaacgccc ttcctgtgcc
     6121 tgaaatcgcc agtcagcagc agattacctg taacggtctt actgacggtg cagtacaact
     6181 ggccgcatcc ggcggacttg aaccttatgc ttacagtatc gatggaggag cattccaggc
     6241 ttccggtgac ttcacgggcc tggctgcagg aatccatgtg ttcgaagtga tggatgacaa
     6301 tggctgtgtt gccggaatca ctgtggagat taccgaacca ttgccgttta cggctgtgat
     6361 tgacggtact gatgatgtaa catgtcacgg cagctctgac ggtgcaatct acgcaaccct
     6421 ctcaggagga actgaacctt acaatatcag ctgggccggg ccggacggat atacggcatc
     6481 caccgcaacc attaccggcc tgggtgcagg tacctacaca ctcactgtct cagataggaa
     6541 taactgcgta acactcagct atatagaagt gattacacag cctgatgcaa ttgagatcac
     6601 atccgtcgtg atttcagata acaatggcta cggagtaacc tgccccgggt ctgcagacgg
     6661 ttcaataacc gtggcagtac agggaggaac gcctccgctt gttctggaat ggaaaggtcc
     6721 tgacggattc acatctgacc agccttcaat ctccatgctt ggtgcaggcg agtacaggct
     6781 gaccatttca gatgcggcag ggtgtaccct cacagcagac tatacactta ctgcacccga
     6841 agaattggat atttcggcca taacgactga tgcctcctgt cctgatacgc ctgatggaag
     6901 tattgacctg acggtctccg gcggttcggg aactctcatc tacctgtgga atgacggtgc
     6961 aactgttgct gacaggacag ctcttctgcc cggagactat accgttgatg tgactgatgc
     7021 cagcggatgt accacaacct ttaaggtcac tgttggtgtc atgggatacg actgcctgag
     7081 gatctatgag atcattacac caaacggtga cggcaggaat gatacatggc agctgcgcaa
     7141 tgcatggctc taccctgatg ccgaagtctt tgtctatacg cgctggggca agctggtcta
     7201 tcattcaagg aatgccactg acgagtggga cggcactttc aacggaaagc tcctgccaaa
     7261 tgactcgtac cattatgtga ttcacctgaa cgacggctct gagccgcgta ctggaataat
     7321 ctcgatcata agcaagtaac cagataaccg gaaggagaaa tgaaaacttt gagaatagta
     7381 gcagtagttg gccttctggc ggttatacct gtcaccagag cgcagcagtt cccgatgtac
     7441 agccagtaca tgatgaacgg ctttcttctc aatccatcgt atgccggaag tgactattac
     7501 accacattcg gcctgacagt ccgcgagcag tggcttaacc tacctcacgc cccaagcacc
     7561 tatgctgcag cttttcagac caggttgctg aatgacagtt acatcacgaa gtcaactgca
     7621 gtcagaaaga agatcagcaa gcctacaaag gggggaaggg taggagtagg aggttatctc
     7681 ttctcggatc ataacggtat catgcacagg acaggcgttc agctggcata tgcgtatcat
     7741 ctccctgtag gagatgagca acaactctct ttcgggttgt cgctcagcgc ataccagtat
     7801 tttgttgata tcggtggtgc cgtaatgcct gatgacgtgc aggatgagtt gctgaacaac
     7861 tatgatcagg tggtttatat ccctgatgcc aatttcgggg tcagttatac cacgaggaga
     7921 tattatgcag gtttcgcaat gaccaacctt ttcaggggta cgctgatgtt gggcaatggc
     7981 agcgaaaaca gccgctccga gcttggccat ttcttcctga ccggaggtat gagatttttc
     8041 ccgggaaccg actggatcat cgaaccgtcg gtaatgctca agtcatcgga catggtgttc
     8101 aaatcgttcc aggttgacct tacgggaagg gtttattaca aggatgatta ctggctgggg
     8161 ctctcgtaca ggaccggcga cgcagtgata ctgatggccg gagtaaaggt ggacaggttc
     8221 tacctgggtt atgcatttga ctttactctc tcagagatcc gcagctatac ctacgggaca
     8281 catgagctga ccctgcttgc ccgctttggt gacaacccga ggcggtacag gtggatcaac
     8341 aaatactgat gaggcacatc ataccctttt tacttattgt cgcagcctgc ctgagctgtg
     8401 acccgcttgc caattctgtc agcggccgac tggccttctc ttcagacacc atctcatttg
     8461 acaccctctt ttccggatat ggctcggcta cccttgaact ccggataaaa aacctggagg
     8521 atacccctgt acttatagac cgcatctggc ttggcggagg ttccggttca ccattcagga
     8581 taaatattga cggccagccc gttgcagagg ctcagaacat tacgcttgcc agaggcgaca
     8641 gcatcttcat ttttatcatt gtgacaatcg atccttccgg tagtgatctg ccactggctg
     8701 tcaccgattc ggtgaatttc ctgtcgggaa gccattccgg cagggttatt ctcgaagcct
     8761 ggggtcagga tgtacgtctt gtcacggagg atataacctc gccggctgta tggagtgaag
     8821 gaaagcctta tgtcatcact ggccatctgc tggttgacac ccttgtgacg ctgacacttg
     8881 aaccgggaac aagagtctac atgcatcacg gggcggaggt gactgtggct ggcacactcg
     8941 ttgcagccgg aacgcccgaa aagagaataa caattgcaac cgacaggctg gaaaaggaat
     9001 acagggatgt gcccggacgg tggaaaggtg tgaagtttca aagctgcagt cgaggcaaca
     9061 tattgaatta tacagatata cgtaatgctc agatagctgt tgaaattgaa ggccatcctg
     9121 cttcggttcc ggacctgacc atgaacagca ccaccctgat gcataacagt gttgcctcac
     9181 tggcagccag taatgctgat ctgtttgcag tcaactccct ttttgcccat tcggggttca
     9241 gtaccgtctc gctctctgag ggcggcaact atgagttcat tcactgcacc atcagtaata
     9301 tatgggagta cagtttccgc tatgagccgg cgcttctgat aagtgccggc gacggaacca
     9361 tgccggcagt gtcagtaatc aattcagtga tcagcggcag cctgccaggt gagttgttga
     9421 tagacgcgcc ggcggctgaa gtggaatcgt ggttcagggc tgacagctca cttataaagg
     9481 ttgacactct gactgctgac tggtattcaa cacctttgtt cagaaatgtt atcacaactc
     9541 agtctccccg cttcattgat gaaaattcat gggatttcag gcctgatacg ctctcacccc
     9601 tccttgaccg tgcagggaag agtgagatga agctatggcc tgccgatatc cggaacaagc
     9661 agcgaccagc cttcgatggt cccgatatcg gggcgtttga aagacaggaa ggagaaaagc
     9721 ggaaagagaa agaaaccggc atggccggca tgcatgtcag gcattgacaa atgtgactat
     9781 tggttgtata tttgttctgc tgctatttta atcagtgcat tattcatcgt gtgatgaggg
     9841 taatactctc agttatggct tttcttgcaa tgatgctgtt gcaggcatcc ggccaggatt
     9901 tcagcgtgat gttttataac gttgaaaacc tgtttgatac tgttgatgat accacaaaga
     9961 atgatgagga attccttcca ggtggatcaa ggaggtggac agcctcgagg tatcacagga
    10021 agctcagcgc tgtcgcgagg gctgtggcgg ttgcagggga gtgggagctc ccttctctgg
    10081 caggtctctg cgaggtggaa aatgaagagg tgctgagaga tcttgtttac ggtactattc
    10141 tttcagccgg caattacgga attgtccaca gggagtcacc agaccccagg ggtatagacc
    10201 tggcgcttct ttaccggcgt gattgttttt catttgtaga ggctgatgca tggctccctg
    10261 cggatgtaga cagcatgcca gtcctgacgc ggaatattct ccatgtgaag ctcaccgcgg
    10321 gagctgatac gctgcatatt gttatctgtc acctgccgtc acggaaagga ggcgtgctgg
    10381 cggctgctga cctgcgggaa agaatggtca ggctggcagg cgccgggatt gactccatca
    10441 tgagcgcctc aggagggagg gcggcggtaa tagtcatggg agatttcaac accgctccgg
    10501 gtgagccgtt gatggaacaa tttgccaggg aggcaggtct ggtcaacctc tcgactgaaa
    10561 cggccggaga ggggaagggt tcatacagat atcagggaac ctgggagatg attgaccaga
    10621 tacttgtctc aaccccgatg acagacagca cgaacgtctt ttatgcattg cccggcactt
    10681 ttcgtgtagt ggatgccccc tttctcctta caactgacgg cacttacccg ggcaaaaagc
    10741 ccttcccgac ctatgggggt tacagctatt caggagggta ctccgatcat cttccggtga
    10801 ctgtcagggt atcagtaaga tgattgtcat accggctcca gtcccatctc acgtcctttc
    10861 cggatcatga attcacgggc ggcctcgtga tcattgggga tgattccgtc gagtatggcc
    10921 tctttgatgg cagatttaat gactcctacc atcgggcccg gaggtatatt gaatgtggca
    10981 attatctctt caccatcaac cggaggctgg aatgtccgga tggcatcctt ctcatctatc
    11041 tccttcagtt tgcggcgcac cagcgcgaag ttctcacgat gcttcagcac cttcattctg
    11101 ttgccggatg tgatgtccgc ctcgcatagc agcatgagat catcaatatc ttcaccggct
    11161 tcgaagagga gcctgcgcac ggccgagtca gtgacttcat cctggacgag tgagacaggt
    11221 ctcaggtgca ggcccacgag cttctgcaca tatttcatcc tgtcattgag aggaagcttc
    11281 aggcgtttga agatttccgg aaccattttg gctcctatga attcatgtcc gtgaaatgtc
    11341 caccccgtgc cctgcacgaa cttctttgtg acaggctttg ccacgtcatg aaggagagca
    11401 gcccagcgca gccagaggtc actgctcttt tctgccagat tgtcaaggac tttcagggta
    11461 tgatgaaaat tatccttatg tcccttacca tccttgtcct caacacctct gagcctggct
    11521 atttccggca ggatgagttc gagcagaccg gtctgctcca gcagcagaaa ccctgctgaa
    11581 ggctttgcag tggtcattat cttgttcagt tcggtggtga tcctttcagc cgatacaatc
    11641 tttattcttt ctgcattgct cctgatcgat gtcagtgtct catcagcgat ccggaaatcc
    11701 agttgcgcgg cgaaccttac ggctctcatc atcctgagcg ggtcatcgct gaaggtggcg
    11761 tcagggtcaa gcggtgtccg gataattccg ttcctgatat catcaattcc cccgaacgga
    11821 tcgagtactt cgccgggatt atccctgtta aggcttattg caagggcatt gattgtaaag
    11881 tcgcgtcttt tctggtcatc ttcaagtgtc cccggagaga cagatggctt gcgggagtcg
    11941 gatgagtatg actctttcct tgcgccaaca aactcgatgt cagagttccg gtacctgaac
    12001 gtagccgtgc cgaaggtcct gaatacggtc accttgatgt cgcggctgat ctcgcgggca
    12061 actgcgcgcg ccattgcagg cccgtcacca agaactacaa tgtcaatatc cctttcgggg
    12121 ttcaccctcc ccattatatg atcacgtaca aagccgccga tgacatatgc ctccacgttt
    12181 tctctgtcag ccacgccggc cactacctca aaaattctgt catcagggaa tatcttcatt
    12241 gtatgtatga cattttagcc gaaaccggtg acaaaattac aatatatagg ttaacttgaa
    12301 gaaaatttga tctccataag cacccggcac accaattgta aagcgtgaac aatatgcgca
    12361 ataatctgtt aaacaataaa aagaacaaat atggcagaac atttgacaaa agagactttt
    12421 cttgagaaag tattcaatta tgagcagaac aaggagtgga aatacaacgg tgacaaacca
    12481 tgtataatcg atttttatgc tgactggtgc gggccatgca ggatggttgc gcctatcctg
    12541 gaggaactgt cgaaggagta cgaaggcaag ctttatgtgt ataagattga tacagaggca
    12601 gaacaggagc ttgcgtcagc attcggaata aggagtattc cctcgttgct cttcgttccg
    12661 atggagggtc agcctcagat ggcaatgggt gccctgccaa aggaagcgtt tgaaaaagcc
    12721 ttcaaggata tatttggaat agaaaaggat caaattgtta ataaataatc tgttttttta
    12781 acaatttcct gcaacaatat cgtaaattaa tcgttctatt tgttgaaggt agttttcaaa
    12841 cagaagagtt agttttctgg aatttaggtt agggtttcga gttgaagctc cgtcacttac
    12901 ggcggagctt ccttgatttt ataccccggg aagttcagaa tgaaataccg gctgcaggaa
    12961 ttacctgagt tcttccatta tcccgtcaat tttctcacct gtctcgcgca gtcggcgccg
    13021 gcatatcttt atgagctcag cggctttcct gacattctct gacagcctgt caatgtcagg
    13081 ttcaccctct tcgatattcc tgaggatctg ttcaataccc ttcatggctt cattgaaccc
    13141 aatttctttt tttgccatta tttcttgttt tttatctctt taacagtgct ttttaccgta
    13201 cccttttcaa aatgggtggt aatgttgtca ccctcctcaa gttcagcggc tgaacggatt
    13261 atgatgctgt tcctggaggt cagtgtaaac cctcttttaa gaacggaggc cgggtcaagg
    13321 tagcgcagtg actgctcaag tctgccggtt ttgtcaccgg ccttttgcag tgcgttcctg
    13381 gctgagcgcc ggagtgtctc ggtgtggtat ccaagtctct cattgttgac ccttacaacc
    13441 agtcttgcgg ttgctgctgt acggttctga aatgacgaga gttgttcatc ggccgcttcg
    13501 agagcacctg ttaccgctga tgccatttca cctgccatac tgattatcct gttttcacat
    13561 tcaagtgttc tgtcaatcag gaatcctgca acggccgtgg gagtcttaag tgctttccat
    13621 gcaaccatgt cagtgactga ctggtccttt tcgtgtccta tgcctgtcag tacaggcagg
    13681 gggaactggg tcacatgaaa tgcaatggca tagttgtcaa accagctcag gtcaatggtg
    13741 gagccgcctc cccttattat tgccaccgcg tcaaacttcc ctgtctcctc tgagattctg
    13801 tcaagagctc cgatgacaga tgcctcagtc tcctcaccct gcatcactgc ttcaaaaagc
    13861 gtggttttaa agacataatc gtagggattg ttcagcaggt gatcggtgaa gtcctggtag
    13921 ccggcagccc ttgatgaaga tacaacggca atattcctcg ggtagagggt aagaggaagc
    13981 cctgagttca tggagagcac tccctcagcg gtaagccttc ttattaccgc ctcgcggcga
    14041 agtgccatat cacctgcggt gtacgccgga tcaatgtctg taatgttcag gctgagtccg
    14101 tatagctcat ggtattccac tgttgccctg aagaggatgg ttattccggc gcggaggggt
    14161 attccggcgg tggcattgaa gagcgtgtta agcgacctgt attttgatgc ccagatggta
    14221 gcccttgccc tggaggtgac tcttcctccg ggtgtgtcac tgccggtgag ttcaaggtag
    14281 cagtgccctg tactattgac cttcatctcg gctatctcgg caaccaccca gaatgcttcc
    14341 ggcagtgctt cgtatatctt gtcgcggacc agccctaaca gctctgtcag tgatattttt
    14401 gcatcccggt tcacctgtct gtacgaatca ttttatagac ctcttccccc tggtctgtta
    14461 ctatcctgac cagataaaag ccctcagcaa ggctgccgag tccgtcaagc ctgaataagc
    14521 gagcggcaaa ggccgggaag tgccttgcca gtatcgtggt gccattcgtt cctgttatag
    14581 tcactgtcag tgaacccggg ggctcatgga accagacaag tatctcgtca gtgaaaggat
    14641 tgggcccggc gctcatcatt gcttcgggtc tgaaagtatg ctcatcctcg agcagcctga
    14701 gtgttttcag gaagtcaggc aacccgtatc cgtacagtga gtcgggtctt gtgtacctgt
    14761 cgctgctttt gcggagcgca ttgataattt caagtggtga ggcatcaggc acagcctgca
    14821 tcagtgaagc cgagaggccg cttatgacag ggcatgagaa agatgtgccg ctgccgctgt
    14881 gccacacccc cggctcaaat tgaaggggta tcagcacacc cggcgccacc atgtccggct
    14941 tgacccggcc gtctgatgag taaccggctg atgaaaagtc agatatggtg aggtcatgat
    15001 taaccgcgcc cacgcacagt acgctgtcgc cgtcagaagg ggcaataatc cgtagccatg
    15061 ccttgttgcg ttcattcccg gcactgttga caacgagtat ccccttggag gaggccatgt
    15121 cagcacccct tgtaatgaaa ggtctgtcac cgtccatatc gctgaacggg taatccatcg
    15181 acgggtcgtc aaattcaaag taaccgaggg atgatgtgat aatatcagct ccagcgctgt
    15241 cggcatattc cgctgcggca atccagtaat cctcctctac gggaaactcg gaaagaacgt
    15301 cttctgtccg gagaagcata tattttgccc ccggggcagt gccattgatg attcccggca
    15361 tcgcccccgc gaggatggag agcactgatg taccatgtgt atgataatca taaacatagc
    15421 tgctgctgtt tataaaatca cgggtggtga ttataccgcc ccgcttcctg agcggttcaa
    15481 gggcttcaat tttgtcagca ttgatgaatc cggcatcaag aactgcaatg gttatgttga
    15541 gtcctgtcaa tcctgactta tgaagcatat cgccgttgag aggcaggcgc gggtcaaatt
    15601 tgtcagtgat catgcccggt tctgttatgc cgtatttgct gtagttcctt ttgacaggat
    15661 caaccggatg ctttacgagc tggatgctgt caataaaggg atactccccg gcgatgtcac
    15721 tctttatggc tccggtagtg gtaaagagag cggtattaag ccactttgac gtgcacctta
    15781 acctgaagcc tgcccctgtg agggtccgta tatgctgact gctgacaggg atgtcgcttt
    15841 ccgtaaccag ggggatgccg tacttttccc tcctggcaat tgcagcaggt gaaagcaact
    15901 cctctggtga gtattcactg atgctgtttg tcttttcgct gaaccagacc ctgtaaaacc
    15961 acagttcgtc atatccggga tatctctgtt gagctttccc cggcataatg cctgcgataa
    16021 caaaaagcaa cggtatgagg gcagatacga ctctgttcat tttattactg atcccgtgat
    16081 atctgtcacc tctattttcc ccttgttctt ttcaagatca tcgaggaatt ttgtctcctg
    16141 gtcagccagt tttgggtcag ccgggacgcc ttccctgtag gtgataatct gcttcaggga
    16201 gccatcttcc ttgaagacca tccagtcacc tgtcctgtta tcctccttat agtggccttc
    16261 atactgcagg ttgccgttag ggtggtagta ggagaagggg ccttcgagcc tgcctgccac
    16321 gaattcagcc ctgaggcata ttgccccgtc ggggtagtac tgcagccatt ctccggtgcg
    16381 gttgcccata tcccatgcca ccttttctgc cagggtgctg tcaggataga acttctgtga
    16441 cagtccgtgc ctgatatcgt tgtgataata ctcctcgcag ataaggtagt gcggccgggt
    16501 ggctgaccag aacttccaga gtccttcctt gctgcgtgct acgtatttgc cttcggcagc
    16561 ctttagcccg tcggggtggt agaatactgc ggatgcctca tccttgctgt ctgagtatgt
    16621 cagttccgaa agaagcagac cctcttcggt atatctcttg aacagtccga ccggtttgtc
    16681 atccctgaag gtgccctggt acatgatatt gccgtttggg tattttttaa cccatgggcc
    16741 ctgctttttc cccccggcat cagtcacatt ttgtgacatg acggtagtgg atagcaacag
    16801 tagtggaaga atgaatatta atctggtttt catgatttcc ttatctggta caaagatata
    16861 accaaatagt ttgttctgtt cgtatctgtg gcatcagaaa aaggaaagcc acggcagcaa
    16921 aagctgccgt ggcttttacc ggtggtttat ttcatataac gtggtgcagt atcagagtct
    16981 aatggtttaa ttaacctgag acttaaaacc tggtgcaaat gcagtacaaa aatcggaaaa
    17041 atgcactaat aataagaatt tgttaaaatg aaaatagcgg caaaacactg ttttaccgct
    17101 atttacttat aattgttaat cgtttttgat tgtcaattat ttaacttcct cgaagtcgac
    17161 gtcagtcacc tgctcgtctc cgtccttgcc gccggttgtt cctccggtgc ctgcctgcgg
    17221 gcctgcttca ggaccggcct gcgggccggg gcctccctga gtctgtttgt aaatatcttc
    17281 ggaggcggcc tgccatgcgg cgttgatctc cgcgagtgca cggtcaatgg ccgtgatgtc
    17341 cttgttctta tgtgcttcgc gaagctggcc gagggcattt tcaatgcgtc ccttcttgtc
    17401 tgaaggaatc ttgtcaccgt actccttgat ctgtttctca gtctggaaga tcatgctgtc
    17461 agcttcgttg accttgtcag ctatctccct tgctgccttg tcggctgcct cattggcctt
    17521 ggcctcatcg cgcatgcggc ggatctcctc ctccttgagt cctgatgaag cctcaatccg
    17581 tatcctctgt tctcttccgg tgcctttatc cttggctgag acattgagta taccgttggc
    17641 gtcaatgtca aaggtaacct cgatctgcgg gacaccgcgc ggggccggcg ggatgccgtc
    17701 aagatggaag cggcctattg tcttgttgcc ggcggccatc gggcgttcgc cctgcagcac
    17761 gtgtatctca accgaaggct ggctgtcgct tgccgttgtg aatgtctcgg tcttgcgcgt
    17821 ggggatggtg gtgttcgact cgatcagtct ggtcatcaca ccgcccatgg tctcaatgcc
    17881 aagtgacagc ggggtgacgt cgagaagaag cacatccttc acttcgccgg taagaacacc
    17941 tccctggatg gcagcgccca ctgcaaccac ttcgtcagga ttgaccccct ttgaaggagt
    18001 cttgccgaag aacttctcaa ctatctgctg gatggcaggt atacgggttg agcctccgac
    18061 gagcagcacc tcatcaatat ccgatgtctt catgccggca tcatcaagcg ccttctggca
    18121 cggtccgatg actgcattaa tgagcttgtc ggtaagtttt tcaaactgtg cccttgtgag
    18181 cgtcttgacc agatgcttgg gaataccgtc aactggcatt atatagggga ggttaatctc
    18241 cgtggttgtg gtgcttgaaa gttctatctt ggccttctct gatgcttcct tcagtctctg
    18301 aagagccatc gggtccttgc ggaggtctat tccttcctcc ttctggaaat catcagcaag
    18361 ccagtcgatt atcaggtgat caaagtcatc gccgccgagg tgggtgtcac cgtttgttga
    18421 cttaacctca aagactccgt cgccgagttc aaggattgag atatcgaatg tacctcctcc
    18481 caggtcgaac actgcgatct taagatcctg tgccttcttg tcaaggccgt aagccagtga
    18541 cgcagccgta ggctcgttga taatgcgctt gaccttcagt cctgcaatct caccggcttc
    18601 cttggtagcc tggcgctgtg agtcgttgaa gtatgccgga acggtaatga cagcctcagt
    18661 gacttcctgg ccgaggtaat cctcggcagt ttttttcatt ttctggagca ctatagccga
    18721 aatctcctgc ggagaatagt tgcggtcatc aatcttaacc ctcgcggtgt tgttgtcacc
    18781 acggacgact tcataggtca tctttttcat ctcgttggaa acctcgtcaa agcttcttcc
    18841 catgaaacgt ttgattgatg aaacggtttt acgcggattg gtgatggcct gtctcttggc
    18901 agggtcacca accttgcgtt cgccgttctc aataaaagca actaccgaag gagtagtacg
    18961 tttgccttca ctattgggta tcaccacagg ctcgttgcct tccatcactg aaacgcagga
    19021 gttggtggtt cccaggtcaa taccgataat ttttcccatt gttattctga tttatatgct
    19081 aattttcttt ttatacgcct ctgaagtgac aatgattgtg ccatagcgct caggcctgag
    19141 ttattatgaa attatgtcat gatgatgcga acctgccggc agt
//
LOCUS       DAOPBK010000025        18774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3049_length_18774_cov_5.023879, whole genome shotgun sequence.
ACCESSION   DAOPBK010000025 DAOPBK010000000
VERSION     DAOPBK010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18774
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3049_length_18774_cov_5.023879"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1551)
                     /gene="mnmE"
                     /locus_tag="PLA17_02570"
     CDS             complement(148..1551)
                     /gene="mnmE"
                     /locus_tag="PLA17_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPK84327.1"
                     /translation="MNLQRDTTIAAPATGIGGAIGIIRVSGPGTFKMVDSLFFPAGDR
                     EILSASGYTVHFGSIRKGDEFIDEVLLTLFRAPASYTGEDSAEISHHASPYIQHRIME
                     LLIDLGVKPAMPGEFTRRAFMNGRMDLSQAEAVADLIAADSAASHRLAATQLRGGFSE
                     GIEKLRKELITFASLIELELDFSEEDVQFADRFAMTETVIKIKKMVDRLISSFRLGNA
                     IRKGIPVVIAGRPNVGKSSLLNILLEEERAIVSDIPGTTRDTVEEVLHVEGLLFRFID
                     TAGLRQGGDEIEELGMERTLNKLKAAEIVLAVTEATDSAGEISDFAASVSEMTGNTEA
                     RIILIINKSDLITRNIASSLKKELEGLCPFPVLFVSAKYSDGFDALKKQLTASVETGR
                     LDNPQYIVTNARHYDALRNVSESLQRVLDGFRDGVPTVLIATDVRQAIYYLGLITGKI
                     LPEDILGEIFARFCIGK"
     gene            1640..2899
                     /locus_tag="PLA17_02575"
     CDS             1640..2899
                     /locus_tag="PLA17_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK84328.1"
                     /translation="MRTLTSVTIIAGLIFLTGCTGNHNKLTSEEIEQGWELLFDGKSL
                     DHWRLYNGEGTGSWGVENGCLAADGTGSDSTGYIISKKEYASFDLKFEWKIEPEGNSG
                     VMYHVVESDRFRTPYLTGPEYQLIDDTGWPEELEEWQKTGADYAMYLPDTSKKVLNEP
                     GQWNSSRILFDNGHVEYWLNGEKIVEFEAWTPEWFALKNSGKWAGAPEYGLATVGYLS
                     LQDHGSRTWFRNIKIKELPSRETGPINLFNGSDLTGWTQYGTEKWYVEDSLLVCESGP
                     DKGYGYLATNMYYKDFDLSLDFRQVSDGNSGVFFRSVIDGTIISGWQVEVAPQGYDTG
                     GIYESYGRGWLVQIPDEKENILIPGEWNRMRIKVVGPDVTVWLNDTEMTRLNDPAIGK
                     ANGRIALQIHDGGGIKVQWKNLVLQPL"
     gene            complement(3036..3815)
                     /locus_tag="PLA17_02580"
     CDS             complement(3036..3815)
                     /locus_tag="PLA17_02580"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPK84329.1"
                     /translation="MKKMLFLLTAISVLSLQAMLAQQSDPVQLMDKSRDLTMSGNIQS
                     TVVLTITEKNGAVRNRKLTMYTKSYDDGTIKRMVKFLDPADVRGTAMLIVDKKASQDD
                     MWIYLPALKRTRRIVSTEKGKSFMSSEFSNADMSSGNNSDFRVKHMPESGKAGTWVIE
                     SVPISDDKADEYGYSRKVTFLEMSSLITKKIDFYNFDNELFKTIEILATQPIAGKEGY
                     VMTDMIAKNHLNGRSSRVKFDQVNTSATIADNVFTVESLAR"
     gene            complement(3821..6100)
                     /locus_tag="PLA17_02585"
     CDS             complement(3821..6100)
                     /locus_tag="PLA17_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPK84330.1"
                     /translation="MTDFIIKYRWIIVAVSFTLAIGIGLLIPSSETDPDIRNYIPEHM
                     DSRVTNDSIEKEFGVQDMIMVLFTDTTILKPGDLRQVREIDRAFSRMDGIGTVNSLFT
                     ARKIEGMDGMMVVDPLIDRIPESAEGLESLKQDILANDFARDIVISSDMTSAAITGTI
                     NTNVSEYETLSKVDSVIAASSGDAQILTGGLPYIRKYITSDVNHDAVILIPLALIIML
                     TVLKLSLGEWRSVFIPFTVVILSTLVTMGMIPLCGWKFSIIMCLAPIILISVANNYGI
                     YIVSRHQELRRSGTPMTGRETVRAIIGTLNMPILFSGLTTVAGLLGLLAHSIIPARYV
                     GVLSATGVSLALAMSLLLIPVLIYFQHSPGMKPPPPLRQRNDLISRAIARISSVVVKR
                     PGRVLLVSIGLTLLVGIGAFFLRIETNQESFFPQKHPVRKAAGIINTKFGGSQTISVM
                     ISGDIKDPVVMNGIDDLTGYLKGLEGVGSVFSISDVVREMSKALYEPEEEEYDMIPSS
                     GEAIAQMFELYNMSGDPDDFKQLMNFENTKAHLLVRLSQTDNETVRNLKDEIKSYTEN
                     FPAKVTIGGYAFIMNDFAQKIIKGQVSSLVFALITVMILLAIIFRSVRGGLTGSIPLA
                     ASIIIQFGVMGYTGIPIDAATALLSSIMIGVGIDFTIQFLWRYKIELANDASHTEAII
                     TTFRTTGRSIVINALTVMAGFSATFISGFLSIRYFGYLTIVSIASCLIYAVIVMPAFM
                     LYFKPSFIEANKKPNIIKQ"
     gene            complement(6107..7453)
                     /locus_tag="PLA17_02590"
     CDS             complement(6107..7453)
                     /locus_tag="PLA17_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84331.1"
                     /translation="MARKFLSAFLVLHLCFSLSAQEKSFTVGGFVRGGGYFSTGDYQH
                     DINAAFGDVALNLNATDNLSFKGFGDLRVRRGQQFGENISTFDVREAWGMYYNNFMSI
                     SLGKKIVKWGKTDIFTPLSRFNPVDYTFRSPDFEDAELGNLLGELIITPAPFFRLSLV
                     ASPFWNPSVLIIKPITLPPSMEVTLPEGLRTGNGYYSWGVRGDFTFSAFDAGLQYYHG
                     PELMPGLSLVSADYTNPLQPAISVEGVPYIISSTGFDFETTVSPFVLRGAAAITTPAE
                     EKRGNEEIPFRQVEWVAGLDWTPGALRITAEYSGKKVMDFYEAPYDPIIGVEPDMQKL
                     MALFNTPGFDPVEFTRLQIEAFNRLYNNQLHEYYHSAGLRIEADLLNGRFFPSVTTVY
                     NFTSKDFTIRPVIKYKPSDGVTLSAGYEHYSGATGGMYDIVDDFMKVLYFAIRVDF"
     gene            complement(7565..>8522)
                     /locus_tag="PLA17_02595"
     CDS             complement(7565..>8522)
                     /locus_tag="PLA17_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK84332.1"
                     /translation="MYSFPFLAFTYGPLLWLYIRHMTRPGLKFSPWNALHFIPFIVFF
                     AVSVIFREEPILDDLSGFLVRDRFIPLRIVYSICFFLSVTVYSVLSFLEIRNHQKRLR
                     DMISYTSAKLSLNWMKILSITFYTIYVLVFILGGIKIFSGILPFDPYVLTFFFIAFFS
                     FVFSFYAIRQPEILDYPAMSPEEENINAANGTARYARSGLRDEQAEEYLNTLLMFMDQ
                     EKPYLNGDLTITDLSRKTGIPRHYITEVLNEKHGRNFFTFVNEYRVREVISRLGDPRY
                     QHYTILAIAFDSGFNSKSTFNTFFKAYTGKTPSDFRQQMEKA"
     assembly_gap    8523..8532
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8533..8779
                     /locus_tag="PLA17_02600"
     CDS             <8533..8779
                     /locus_tag="PLA17_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84333.1"
                     /translation="ISRTVRFTRAKIISIQKRNTHAVIILLAVVNGLRVARRSPAKND
                     CVIPIYNTGSMKFISAGICSEINKRIKKYLIFEIGGR"
     gene            8823..9032
                     /locus_tag="PLA17_02605"
     CDS             8823..9032
                     /locus_tag="PLA17_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84334.1"
                     /translation="MIVQRFTGREGTFHAGQTALKGRRTGHAAITDGSGDTAGTRMKT
                     VAGCGIHLVRSPDDIGSTVARVLFK"
     gene            9065..9811
                     /gene="fabG"
                     /locus_tag="PLA17_02610"
     CDS             9065..9811
                     /gene="fabG"
                     /locus_tag="PLA17_02610"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPK84335.1"
                     /translation="MKLLEGKTAVITGGSRGIGKEIALRFATEGANIAITDIVDNEEF
                     ATTIREISAHGVKAKGYISDASLFLDAEKVIEEIVKDFGTVDILVNNAGITRDTLLMR
                     MTEEQWDLVLKVNLKSVFNLTKAVIRPMMKQRNGAIINMSSVVGVSGNAGQANYSASK
                     AGIIGFTKSVAKELGSRNIRCNAIAPGFILTEMTNKLPEDVKNDWIAKIPLKRGGQTG
                     DVANVALFLASDLSSYVSGQVISVCGGMAM"
     gene            10138..10209
                     /locus_tag="PLA17_02615"
     tRNA            10138..10209
                     /locus_tag="PLA17_02615"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10170..10172,aa:Thr,seq:cgt)
     gene            10310..10382
                     /locus_tag="PLA17_02620"
     tRNA            10310..10382
                     /locus_tag="PLA17_02620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10343..10345,aa:Gly,seq:ccc)
     gene            10518..10841
                     /locus_tag="PLA17_02625"
     CDS             10518..10841
                     /locus_tag="PLA17_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84336.1"
                     /translation="MKKIAFVCLLSFIIISCKNHYDEYPDNPAWLNARIAHMETVDYY
                     YGTKVFLYEWHSAFYYWISIPISSCMMCEFYNYAGDEVLTPDNIDDFQKNGKMIKIVW
                     QRDKF"
     gene            complement(11007..11660)
                     /locus_tag="PLA17_02630"
     CDS             complement(11007..11660)
                     /locus_tag="PLA17_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84337.1"
                     /translation="MGVRVGMTSEQNLQALTDPSLYSTISGFDDRYQGVRGSPRMLDT
                     LLPSTVKLKGEPELFEVKIDIDVVRNTMLFVLSSSGDLLEVASSWIEELIIHDGERDL
                     LFRTTDGLKFDRELRGNRFYQVLYEGPHLFIKIPDRDFLEASFTGLYNPDKRYDEYVP
                     NDKYYLMGSDSILHRVQLNRKSLSRLFPSWSKQINSVFRENSEDPEQQVIRLLEEVQ"
     gene            complement(11911..12843)
                     /locus_tag="PLA17_02635"
     CDS             complement(11911..12843)
                     /locus_tag="PLA17_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPK84338.1"
                     /translation="MKYIGLNSQIWRNNFKSVILLILFPVVILGLVWLFVFLVQPLNE
                     YRIERANQLFLTSLPWVLAGVGVWFVIAWFSHTGMIERATGSRPLSRKENKRIYNLVE
                     NLCIASGMPMPKVNIIDDDSLNAFASGINTSTFTVSLSRGIVDKLDDAELEAVIAHEL
                     THIRNRDVRLLIVSIIFVGIFAFLTEALVRSVRFSGGSNKKDGRVVIVALLLAAIGYF
                     LSSIFRFAISRQREYMADAGSAMMTKNPLALASALEKISVDSQIEAVKRNDVAQMFID
                     NPRDKKVRPVSFSIGTLFMTHPPIEKRIALLRQF"
     gene            complement(12849..13403)
                     /locus_tag="PLA17_02640"
     CDS             complement(12849..13403)
                     /locus_tag="PLA17_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPK84339.1"
                     /translation="MFYALLIIIAVLALLAVSLYNRLVRLRNNRENAFADIDVQLKQR
                     LDLIPQLVEAVKAYMKHESTVLVEITKARAGAMEAKGIDEKIAADGKLSSALQGLRIA
                     VEAYPELKASTSFMQLQEEISDIENKLAAARRFFNSATKELNNAVEVFPSNLIAGMFG
                     FKREAMYDLADNRAQAEETPRLNF"
     gene            complement(13556..14206)
                     /locus_tag="PLA17_02645"
     CDS             complement(13556..14206)
                     /locus_tag="PLA17_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK84340.1"
                     /translation="MKDEKSVIGEVISFVKEAMLEHESGHGWSHIERVVRMALHIRES
                     EGRGERLTVELGALLHDVGDHKFRAHDGPAEIRRLLERLGVDSKVTDEVVRINENISF
                     SKGRRPDSAGIEFRIVQDADRLDAIGAIGIARAFSYGGFSGNEIYDPRISIADPPLLA
                     GTGSKSASTIHHFYDKLLLLKDLMNTPTGRRLAEERHDYMVRYLERFFMEWNTGSM"
     gene            14278..15522
                     /locus_tag="PLA17_02650"
     CDS             14278..15522
                     /locus_tag="PLA17_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPK84341.1"
                     /translation="MNSAEIITIGDELLIGQTVDTNSAWMGMQLSMRGIQVNRIVSIS
                     DRREEIIAALDESLRRVPLVLITGGLGPTSDDITKETLTEYFGTKLVMSQEVLDEVTN
                     RITRRNLEMNENNRRQAMVPESCRVLANHAGTAPAMLFEKEGRIIVSMPGVPSEMKHI
                     MHEHVLPMISSMTGGHTIIHRNIMTFGTFEAKLAERLEGFEKELPAMVKLAYLPSHGV
                     IKLRLTGSGDDPELVRRTEEEQVRKLYSIIPDVIYGEDEITLEEIVGKLLLDNNQTVS
                     TAESCTGGKIASMITSVPGSSAWYRGSVVAYDNSIKAGVLGIDPDIISRYGAVSEETV
                     RAMAERMRSIAETDYAVAVTGIAGPTGGTPEKPVGTVWIAVASAAGVVAEKHLFADDR
                     QINIFRSATNALNLLRKHIISR"
     gene            15641..15880
                     /gene="rpmB"
                     /locus_tag="PLA17_02655"
     CDS             15641..15880
                     /gene="rpmB"
                     /locus_tag="PLA17_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPK84342.1"
                     /translation="MSKVCQVTGKRAMVGNNVSHSKRRTKRKFMVNLFKKRYFLAEEN
                     RWITLKVSAAGMRKINKNGLSSVLKEAKEKGYIIN"
     gene            15898..16086
                     /gene="rpmG"
                     /locus_tag="PLA17_02660"
     CDS             15898..16086
                     /gene="rpmG"
                     /locus_tag="PLA17_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPK84343.1"
                     /translation="MAKKTKGNRVQVILECTEHKESGQPGTSRYITTKNRKNTPDRIE
                     RRKYNPILKKVTVHREIK"
     gene            16098..16253
                     /locus_tag="PLA17_02665"
     CDS             16098..16253
                     /locus_tag="PLA17_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HPK84344.1"
                     /translation="MAKKAVATLKAGTGKAFTKCIKVVKSEKTGAYQFKEEVVNNENI
                     NDFFTRK"
     gene            16338..17303
                     /gene="ftsY"
                     /locus_tag="PLA17_02670"
     CDS             16338..17303
                     /gene="ftsY"
                     /locus_tag="PLA17_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPK84345.1"
                     /translation="MALFGLFKKEEKEILDRGLSKTRDSVFSKLTRAVAGKSKIDDEV
                     LDNLEEVLVTSDVGVETTLRIIDRVSKRVARDKYLNTTELNAILREEIAGLLEENKVT
                     HDVSSGFDAPLPAKPYVILVVGVNGVGKTTTIAKLAYNFRKAGRSVLLGAADTFRAAA
                     IEQLKIWAERAGVPMVSQQMGADPASVAYDTLKSAVASETDIVLIDTAGRLHNKIGLM
                     NELSKIRRVMEKVIPFAPHEVLLVLDGSTGQNAFEQARQFTAATEVNALAITKLDGTA
                     KGGVVIGISDQFRIPVKYIGVGEKMDDLRIFNRYAFVDSLFSDRT"
     gene            17303..18598
                     /gene="rimO"
                     /locus_tag="PLA17_02675"
     CDS             17303..18598
                     /gene="rimO"
                     /locus_tag="PLA17_02675"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPK84346.1"
                     /translation="MKRNRVNIVTLGCSKNTVDSQKLMRQLAAGGYSVTAGSADPADI
                     VIINTCGFINDAKEESVDTIIRYAEAIRREGHGRLFVMGCLVERYRNELQHELPEVDG
                     WFGVNRPQEIISRLGLAYREELTGERVITGPAHYAYLKVSEGCERTCAFCAIPDIRGR
                     YISAPLEKLVEEARLLASQGVKELILIAQDLTLYGTDLYGRQMLPGLVRRLAEIDSLE
                     WIRLHYLYPAGFPMEILEIMQENPKICRYIDIPVQHISDKVLKMMRREHDSAGTKRLL
                     ADIRRILPDAAVRTTVIAGHPGETEKEYRELLEFIGEYRFDRLGAFRYSHEENTHGAL
                     NYKDTIPERTKERRVAGIMKLQQEISAARNASMTGKVIKVIIDRREGLFHVGRSQHDS
                     PEVDQEILVRAAGKIEPGTFADVKITGSTEYDLYAELLQ"
BASE COUNT         5057 a         4318 c         4642 g         4747 t
ORIGIN      
        1 ttgttgcccg gcaactatat tatttttctt gggcaacaat ctggcaacaa agataggtaa
       61 aataaaggaa attgggcaac aaataaaaga aaataaatta cttaattgct aataaaaaca
      121 agctgttgga aaaaatgaga aagataatta ctttccaata caaaacctcg caaagatctc
      181 tccaagtata tcctcgggta atatctttcc tgttatcaac ccgaggtaat agattgcctg
      241 tcttacatca gtggctataa gaaccgtggg taccccgtcg cggaatccgt caagcaccct
      301 ctgaagactc tctgaaacat ttctgagagc atcataatgt cttgcatttg taacgatgta
      361 ctgaggattg tcgagcctgc cggtctcgac tgaggctgtc agctgcttct tcagagcatc
      421 gaaaccatct gaatactttg ctgaaacgaa gagcacaggg aaggggcaga gtccctccag
      481 ttccttcttc agtgatgaag caatatttcg tgtaataagg tcagacttgt tgatgatcaa
      541 tattatcctg gcttctgtat tacctgtcat ctcactgact gaagcggcaa agtcggatat
      601 ctcaccagcg ctgtcagtgg cttccgtcac tgccagaaca atctctgctg ccttcagttt
      661 attcagcgta cgctccattc ccagttcctc aatctcatca cctccttgcc tcagcccggc
      721 agtgtcaatg aaccggaaga gaagtccttc cacgtgcaat acctcttcaa ccgtatcacg
      781 cgttgtgccg gggatatccg acacaattgc acgctcctcc tcaagcagta tgttgagcag
      841 cgacgacttg cccacattcg gtctgccggc aattaccacc gggatgcctt tcctgattgc
      901 gttgccaagc ctgaatgagg agatcagcct gtcaaccatc tttttgatct taatgactgt
      961 ctcggtcatt gcaaaccggt ctgcaaactg cacgtcctcc tcgctgaaat cgagctcaag
     1021 ctcaatcagg gaagcaaagg ttatcagttc cttcctcagt ttttctattc cttcagagaa
     1081 gcctccccgc aactgtgttg ctgcaagacg gtgtgacgcc gccgaatcgg cagcaatcag
     1141 atcagctacc gcctcggcct gcgagagatc catcctgccg ttcatgaaag ccctccgggt
     1201 aaactcaccc ggcatggcag gcttcacacc cagatcaata agcaactcca tgatcctgtg
     1261 ctggatgtaa ggtgatgcat gatggcttat ctcagcagag tcttctccgg tataagaggc
     1321 aggagccctg aacaacgtca gcagaacctc atcaatgaac tcatcacctt ttctgatact
     1381 tccgaaatgg acagtataac cagaggctga gagaatctcc ctgtcgcctg ccgggaaaaa
     1441 caatgagtca accattttaa atgtgccggg accgctgaca cggattatac caatggctcc
     1501 gccaataccg gttgccggcg ctgctatcgt tgtgtctctc tgtagattca tcggcatttt
     1561 ttttgcgtcc cggcaaagat aatgattgaa gataagttat tatatgctaa tttagaagct
     1621 tgactaacat ataataacta tgagaacatt gactagtgta acaattatcg ccggtctgat
     1681 tttcctgaca ggatgcaccg gtaaccacaa caagctgact tctgaggaaa ttgagcaggg
     1741 ctgggaactg ctgtttgacg gcaaaagcct ggatcattgg agattatata acggcgaagg
     1801 caccggctcg tggggtgttg aaaacggatg cctggctgct gacggtaccg gcagcgacag
     1861 cacaggttat ataataagca aaaaggaata tgccagtttt gatctgaagt ttgagtggaa
     1921 gattgagccc gaaggcaaca gcggagtaat gtatcatgtg gttgaatctg acaggttcag
     1981 aacaccctat ctgacaggtc ctgagtacca gctgatagat gacaccggct ggcccgaaga
     2041 actggaagag tggcagaaaa ccggtgccga ttacgcgatg tacctgcctg atacttcaaa
     2101 aaaggtactc aatgaaccgg gccaatggaa cagttcaaga attctgtttg acaacggcca
     2161 tgtggaatac tggctgaacg gagagaagat agttgagttt gaagcctgga ctcctgagtg
     2221 gtttgccctc aagaacagtg gcaaatgggc aggagcaccc gaatacggac ttgcaaccgt
     2281 tggttacctc agccttcagg atcatggttc cagaacatgg ttcaggaaca ttaaaatcaa
     2341 ggaactgcct tcaagggaaa ccggcccgat caaccttttc aatggctctg acctcaccgg
     2401 ctggacacag tacggaactg agaagtggta tgtagaggac agcctgctcg tgtgtgaaag
     2461 cggtccggat aagggttacg gataccttgc cacgaacatg tactataagg atttcgatct
     2521 ctcgctcgat ttccgccagg tttcagacgg taacagcgga gtcttcttca ggtcagttat
     2581 tgacggaaca attatctcgg gctggcaggt agaggtagct ccacagggtt atgatacagg
     2641 cggtatttat gagtcatacg gaagagggtg gcttgtacag atccctgacg agaaggagaa
     2701 tattcttatc cccggtgaat ggaacagaat gaggattaaa gttgtcggcc ctgatgtgac
     2761 cgtatggctc aatgatacgg aaatgacacg gctcaatgac cctgctatcg gaaaggccaa
     2821 tggcaggatt gcactacaga ttcatgatgg gggaggcata aaggtgcaat ggaaaaacct
     2881 ggttctgcaa cctctgtaag ccaccctgac cattgggtgc ctctatgagg ccgaaagata
     2941 cggaaagaca ttgcgaagcc ccgttactta atatttgtga gcggggcttc gtaattagtt
     3001 aaattattgt taatcagcga ttaatgacag gcaactcacc tcgccaggct ctcaaccgta
     3061 aaaacattat cagcaatggt tgcagaggtg ttaacctggt caaatttgac tctggagctt
     3121 cgcccattga gatggttttt ggctatcatg tcagtcatca cataaccctc tttgccggct
     3181 ataggctggg tggccagtat ctcgattgtt ttgaacagtt cattgtcaaa attatagaaa
     3241 tcgatcttct ttgtaatgag gctgctcatc tcaagaaaag taacctttct tgaataaccg
     3301 tattcgtcag ctttgtcgtc acttattggt acgctctcta ttacccatgt gccggccttt
     3361 cctgattcag gcatgtgctt cactctgaag tcggaattgt ttccgcttga catgtctgca
     3421 tttgagaact ctgagctcat gaaactcttt cctttttcag tgctgacaat acgccgggtc
     3481 cttttcagcg ccggaagata aatccacatg tcgtcctggc ttgccttttt gtcaacaatg
     3541 agcattgccg ttccccgcac atcggccggg tcgaggaact taaccattct cttgatcgtt
     3601 ccgtcatcgt atgatttggt gtacatagtg agcttccggt ttctgacggc accgttcttt
     3661 tcggtgattg tgagtacaac cgttgactga atatttcctg acatagtcag gtcacggctt
     3721 ttatccatca gttgcacggg atctgactgc tgtgccagca tggcctgcag tgacagcacc
     3781 gatattgctg ttaagaggaa taacatcttt ttcattctta ctattgtttt ataatgtttg
     3841 gtttcttatt tgcctcaatg aatgaaggct tgaaataaag catgaatgcc ggcatgacta
     3901 taacggcata gataaggcat gaagctatag agacgatagt caggtaaccg aaatatctta
     3961 ttgacaggaa accggagata aatgtagccg agaatccggc catcacggtc agcgcgttga
     4021 taactatact ccttccggta gtcctgaagg tggtaattat agcttcagta tgagaagcat
     4081 catttgccag ttcaatcttg tatctccaca ggaactggat tgtaaagtcg attccgacac
     4141 caatcattat tgaggagagg agggcagtgg cagcgtcaat tggaatgcct gtgtagccca
     4201 ttacaccaaa ctggattatt attgaagctg ccagggggat tgatcctgta agcccgcccc
     4261 tgacagacct gaagattatt gcaagaagaa tcatcactgt tatcagggca aagacaagcg
     4321 atgagacctg ccctttaata atcttctgag caaaatcatt catgatgaat gcataccctc
     4381 cgatagtcac ttttgcaggg aaattttcag tatagctttt tatttcatct ttaagatttc
     4441 tgacggtctc attgtctgtc tgtgacaggc gaaccagcag gtgggccttt gtgttttcaa
     4501 agttcatcag ctgcttaaaa tcgtccggat ctccgctcat gttatacagc tcgaacatct
     4561 gagcgatggc ctcgccggat gaaggtatca tgtcatattc ctcttcctcc ggctcataaa
     4621 gtgccttgct catctccctt acaacatccg agatggagaa gacgctgccg acaccttcca
     4681 gacccttcag gtaaccggta agatcatcaa tcccattcat tacgacagga tcctttatat
     4741 caccgcttat catcaccgag atggtctgtg atccgccgaa ttttgtattg attattcctg
     4801 ctgcctttct caccggatgt ttttgcggaa agaaactctc ctgatttgtt tcaattctaa
     4861 ggaaaaatgc accgatgccc acaaggagtg tcaatcctat tgacacgagc aatactcttc
     4921 ccggacgctt gacgactact gacgagattc ttgcaattgc acgggaaatc aaatcgtttc
     4981 gttgacgcag cggtggcggt ggtttcattc ccggagaatg ctggaaatag atcagaacag
     5041 gtatcagcag cagactcatt gccagtgcca ggctgacgcc cgttgctgac agtacgccaa
     5101 catacctggc cggaattatt gaatgtgcaa ggaggccaag caatccggca actgtggtca
     5161 gtccgctgaa caggatgggc atattaagtg tgcctattat tgccctgact gtctccctgc
     5221 ctgtcatggg tgtgccagac cttctgagtt cctgatgcct ggatacaatg tatatgccgt
     5281 aattatttgc gactgagatc agaatgatag gggcaaggca catgattatg gagaatttcc
     5341 atccacacag tggaatcatg cccattgtca caagtgttga gagtatcaca accgtgaagg
     5401 gaatgaagac acttctccac tctcccaggg agagtttcag caccgtcagc atgattatca
     5461 gagccagggg tatcaggatc actgcatcat gattcacatc cgatgtaatg tatttcctta
     5521 tgtatggcaa tccgcctgtc aggatctgtg catccccgct gctggctgca atgactgaat
     5581 cgaccttgct cagagtttca tattcagata catttgtgtt gatggttcct gtgatggctg
     5641 ccgaagtcat gtctgatgat atgacaatat cacgggcaaa gtcattcgcg agtatgtcct
     5701 gtttgaggct ttccaggcct tcggccgatt cgggtattct gtcaatgagc ggatcaacca
     5761 ccatcattcc atccatgcct tcaatcttcc ttgctgtaaa gagagagttg accgtaccta
     5821 tcccatccat ccgcgagaaa gccctgtcaa tctccctgac ctgcctcagg tcaccaggct
     5881 tgagaattgt agtgtctgta aataatacca tgatcatatc ctggacaccg aattcctttt
     5941 caatggagtc attggtgacc ctggaatcca tgtgctcagg gatataattc ctgatgtcag
     6001 gatctgtttc cgatgatggt atgagaaggc ctattccgat tgccagtgta aacgagacgg
     6061 ctactataat ccatctgtat ttaattataa agtcagtcat agtatcttaa aaatctaccc
     6121 tgattgcgaa gtataatact ttcataaagt cgtcaacgat atcgtacata ccgcccgtgg
     6181 ctccactgta atgctcatat ccggcgctga gtgtcacccc gtcagagggt ttgtatttga
     6241 ttaccggacg aattgtaaag tcctttgatg tgaagttgta aaccgtcgta acagaaggaa
     6301 agaatcttcc attgagaaga tcagcctcaa ttctcagtcc ggcagagtgg tagtattcat
     6361 gcagctggtt gttatacagt ctgttgaatg cctcaatctg cagccgggta aactcaaccg
     6421 ggtcgaatcc gggtgtgttg aagagtgcca tcagcttctg catgtcaggc tccacgccaa
     6481 taatagggtc ataaggtgcc tcgtaaaaat ccattacctt tttgcctgag tactctgccg
     6541 ttatccttag tgcgccagga gtccagtcca gccctgcgac ccattcaacc tgtcggaatg
     6601 gtatctcctc attgcctctc ttttcttcag ctggagtggt gatggcagcg gcgccccgca
     6661 acacaaacgg agaaactgtt gtctcaaaat cgaaccctgt actgctgatt atatatggca
     6721 cgccctcaac cgagatggct ggctggagcg gatttgtgta atcagcactg acaagcgaga
     6781 gcccgggcat cagttccggg ccgtgataat actgcaggcc tgcatcaaat gcactgaaag
     6841 tgaagtcacc ccttacgccc caggagtagt atccgttgcc tgtcctgagt ccctcgggta
     6901 gtgtcacctc cattgacggg ggaagcgtga ttggcttgat aatcaagact gacggattcc
     6961 agaaaggtga tgctacgaga gataacctga agaatggggc aggggtaata atcagttcgc
     7021 ccagaaggtt tcccagttct gcatcctcaa aatcaggtga ccggaatgta tagtcaaccg
     7081 ggttaaatct cgacagaggg gtaaagatat cagttttccc ccacttgaca atctttttcc
     7141 cgagggagat gctcatgaag ttgttgtagt acatgcccca ggcttccctc acatcaaaag
     7201 tgctgatgtt ctcgccgaat tgctgtcccc tccgcacccg aagatcaccg aaacccttga
     7261 aactcaggtt gtctgttgcg ttaagattga gagcgacatc gccaaaggcg gcattgatgt
     7321 catgctgata atcaccggtg ctgaaatatc ctccacctct gacaaatcct ccgactgtga
     7381 aactcttctc ctgtgctgac agggaaaaac agagatgtaa aaccaaaaat gcagacaaaa
     7441 atttccttgc catagttttt tttatgacaa agatggcctg ctatagtgtg ccggtcgtcc
     7501 attccatgcg gaccgaacct gtcatggcgg cggaagaggt tcgaaccgag cggacaagac
     7561 gtctctatgc tttttccatc tgctgccgga aatcgctggg tgtcttgcct gtataggctt
     7621 taaaaaaggt gttgaaggtt gatttcgagt tgaaccctga gtcgaaggct attgcaagaa
     7681 ttgtatagtg ctggtacctg gggtcaccga gtctgctgat tacctccctg accctgtact
     7741 cattcacaaa ggtgaagaaa ttccttccat gcttttcatt caggacttcg gtaatgtaat
     7801 gtctgggtat cccggttttt cttgaaagat cagtgattgt aaggtcaccg ttaagataag
     7861 gcttctcctg gtccataaac atgagcaggg tgttcaggta ctcttcggcc tgttcgtcgc
     7921 gcagtccgct tctggcatac ctggctgtgc cgttggcagc gtttatgttc tcttcttcag
     7981 ggctcattgc cggataatcg agtatttcag gctgccggat tgcataaaag ctgaatacga
     8041 aggagaagaa tgcgatgaag aagaatgtaa gcacataagg atcaaaggga agtattccgg
     8101 aaaagatctt tattccgccc agtataaaga caagcacata gatggtataa aatgttatag
     8161 agaggatttt catccagttc agtgacagtt tggccgatgt atatgagatc atatcgcgga
     8221 gtcgcttctg gtgatttcgt atctccagga acgagagcac gctgtagact gtcacagaga
     8281 ggaagaaaca gatgctgtaa acaattctca gcgggatgaa tctgtctctc accaggaatc
     8341 cgctcaggtc atccaggatt ggctcttcgc gaaatataac cgagactgcg aagaagacaa
     8401 taaagggaat gaagtggagt gcattccatg gagagaattt caatccgggc cttgtcatgt
     8461 gccggatgta cagccagagc agaggtccgt aggtaaaggc caggaaaggg aatgaataca
     8521 tannnnnnnn nncatatcca ggaccgtgcg gtttacaagg gcaaagatca tctctataca
     8581 aaagaggaac acccacgctg ttattatcct gctggctgtt gtaaacggtc ttcgagtcgc
     8641 aagaagaagt ccggcaaaaa acgattgcgt tattcctata tataatactg gttcgatgaa
     8701 attcatatct gctggaatat gcagcgaaat taacaaaagg attaaaaaat accttatttt
     8761 tgagatagga ggcaggtaaa gaagaccaca atttgagtat cccggttaac ccgtagttga
     8821 aggtgatcgt tcagcgtttc accggcaggg agggaacatt tcatgccggt cagaccgcac
     8881 tgaaagggag gcgcacgggt catgccgcaa taacagacgg ttccggtgac actgccggaa
     8941 ccaggatgaa aaccgtagcc gggtgtggca tacatctggt gagatcacct gatgatattg
     9001 gttcaacagt tgcacgggtg ctattcaaat aaaatcatat atttgtcccc aaaattttac
     9061 agaaatgaaa ttactcgaag gaaaaacagc agtgatcaca ggtggatcaa gaggcatagg
     9121 caaggagata gcattgcgct tcgctaccga gggagccaat attgctatta ccgatatagt
     9181 tgacaatgaa gaatttgcca ctactatcag ggagatatcc gctcacggtg taaaggctaa
     9241 gggttatata tcagatgctt cactttttct tgatgcagaa aaagtgattg aagagatagt
     9301 taaggatttt ggcactgttg acatccttgt aaacaatgcc gggataacac gtgatactct
     9361 cctgatgagg atgacagagg agcagtggga cctggtgctg aaagtgaacc ttaaatcggt
     9421 cttcaatctc accaaggctg taatcaggcc gatgatgaag caaaggaacg gtgcaatcat
     9481 caatatgagc tcagtagtag gtgtcagcgg caatgcagga caggcaaact attcagcctc
     9541 caaggcaggt atcatcgggt tcaccaagag tgttgccaag gagcttggtt cgcgcaatat
     9601 aaggtgtaac gccattgcac cgggctttat acttacggag atgacgaaca agcttccgga
     9661 agatgtaaag aacgactgga tagcaaagat tcccctgaag cgtggcggac agaccgggga
     9721 tgtggcgaat gtggctcttt tcctggcttc tgatctctct tcatatgttt ccggccaggt
     9781 catcagtgtc tgtggcggta tggcgatgta atagctgcat cctgacagat caatgcagac
     9841 cggggctcca ggaagctcca caatcgtaaa acctgactgt tgcattttga tagatttacg
     9901 cagaccagag aggcatgacc tgcttccaga aaaagtcatt taaaaatccc ctgaaagctc
     9961 atttacattt tgacagagga tattttaacc aatgctgtaa atttttatca agcagttaag
    10021 taatttgtta tatttgcacc ggcagggcgc tattccctca agcacgagga aatgcagcga
    10081 gacataatta ttccacactt atttcttatg cgccctgctt ttttttattg tacgcatgcc
    10141 tccttagctc agtggtagag cagctcactc gtaatgagca ggtcgtgggt tcaactccca
    10201 tgggaggctc agagagaggg agttggatgg agagctcacg aaccatgaaa gtttcaactt
    10261 ccatgcgagg ctcagagaga aggaattgga aggggataag gaataatagg cgggaatagc
    10321 tcagtcggta gagcatcagc ttcccaagct gagggccgcg ggttcgaatc ccgtttcccg
    10381 ctcgttgaca atcaggagtt tagccttgta acggggcaag ctcctgattt ttgtttatta
    10441 atatgaaaaa tttgtcgaac tttggtatta attggattta tttttgatgt gaaaatatta
    10501 atactaaacc tgatgccatg aaaaagatag catttgtatg tcttctctca ttcatcatta
    10561 tatcctgcaa aaatcattat gatgaatatc ctgataatcc tgcctggtta aatgccagaa
    10621 ttgcccatat ggaaacggtt gattattatt acggtacgaa agtattttta tacgaatggc
    10681 attctgcctt ctattattgg atatccatac ctatatcatc ctgtatgatg tgcgaattct
    10741 ataattatgc aggagatgag gtattgacac cagataatat tgatgatttc cagaaaaatg
    10801 gcaaaatgat taagattgtc tggcaaagag ataaattcta accagacacc gttagtttgt
    10861 taaaacctct tccagcctga aacaatagta tcagaaccct attcgccggc atatgtgcag
    10921 agtcacacgt ggcagcaatt ccggatggcc tgtagccatc tttcattgcg cgtatccgca
    10981 tctctttttg aatggcatgg agccagctat tgcacctcct ccagcagcct gataacctgc
    11041 tgctcaggat cctccgaatt ttccctgaaa acgctgttga tctgttttga ccatgacggg
    11101 aacaatctgg agagcgattt cctgttgagc tgaaccctgt gtagtatgga gtcgctgccc
    11161 atcaggtagt acttgtcatt cgggacatac tcatcatacc gtttgtcagg attgtataaa
    11221 cctgtaaagc tggcctcaag gaaatccctg tcaggaattt tgatgaatag atgcggaccc
    11281 tcgtataaaa cctgataaaa cctgtttcct ctgagttccc ggtcaaattt aagtccatca
    11341 gtagttctga acagcaggtc cctctctccg tcatgaataa tcaattcctc aatccacgag
    11401 gaggcaactt caagaagatc accggaagat gacaatacga acaacattgt atttctgaca
    11461 acatcaatgt cgattttcac ctcaaaaagt tccggttcac ctttaagttt taccgtggaa
    11521 ggaagaaggg tatcaagcat tctgggtgag cccctgactc cctgatatct atcatcaaaa
    11581 cctgatattg tgctgtagag tgagggatca gtaagcgcct gcaggttctg ttcacttgtc
    11641 atgccaactc tgacacccat tttatcctga cctccggagt taacagatat caggatcaaa
    11701 gaaacgaata agcaacctat tttcttcata gcctgagttt ttgtgtttac agcaagcccg
    11761 gaataccgac tgatttatac aaatatacga aatcagcctt atgcattgct tgaatgattc
    11821 cataaaaact gagaattcat tcacctgttt ttcaggcatc aggatgcgga gaatgtattc
    11881 gggctggaag agtagggtgg tgaatcatca tcagaactgc ctgagcaatg caattcgttt
    11941 ttcgatggga gggtgggtca taaacagggt accgattgaa aatgataccg gccttacttt
    12001 cttgtccctt gggttgtcaa tgaacatctg tgcgacatca tttcgcttga ctgcctctat
    12061 ctgcgaatca acagatatct tttcaagcgc tgatgcaagt gcaagaggat ttttggtcat
    12121 cattgccgaa ccggcatctg ccatgtattc gcgctgacgc gaaatggcaa accggaagat
    12181 cgacgagagg aagtacccga tagcggcaag caaaagggca acaattacca ccctgccgtc
    12241 ttttttgttg ctgcctccgc tgaagcgaac cgaacggacc agtgcctcgg taagaaaagc
    12301 aaagattccg acgaaaatta tggaaacgat caggagcctg acatcgcggt tgcggatatg
    12361 agtcagctca tgggcaatca cggcttcaag ctcggcatca tcaagtttgt cgacaatccc
    12421 ccgtgataag gagacggtaa aagtagaggt gttaattccg ctggcgaagg cattaagcga
    12481 gtcatcgtcg attatattga cctttggcat tggcatgccg ctggcaatgc acaggttctc
    12541 aaccaggttg taaatcctct tattctcctt cctgctgagg ggtctggagc cggttgccct
    12601 ttctatcatc cccgtatggg agaaccatgc aatgacaaac cacactccaa cgcctgcaag
    12661 cacccatggc aatgaagtga gaaagagctg gttggcccgt tcaatcctgt actcattcag
    12721 gggctgtaca aggaaaacaa agagccacac caaccccagt atgacaacgg gaaagagaat
    12781 gagcaaaatg acagatttga agttattcct ccagatctga ctgttaagcc cgatgtactt
    12841 cattccggtc agaagttcag cctgggagtc tcctctgcct gtgccctgtt atcagcaaga
    12901 tcatacattg cttcgcgttt gaagccgaac atccctgcaa taaggtttga ggggaatacc
    12961 tcaacggcat tgttcagttc cttcgtggcg gagttgaaga accggcgtgc tgcagcaagc
    13021 ttattctcaa tgtcagatat ttcctcctgc aactgcatga aactggtgct tgccttcagc
    13081 tccgggtaag cctccacggc aatcctcagt ccctgcaggg ctgaagagag tttgccatca
    13141 gctgcaatct tctcatcaat tccctttgcc tccatagcac cggcacgggc tttggtgatc
    13201 tcgacaagca cagtgctttc gtgtttcata taagccttca ccgcttcaac cagctgcggt
    13261 atcaggtcaa gcctctgctt cagctgtaca tcgatatcag caaaggcgtt ttccctgttg
    13321 ttcctgaggc gtacgaggcg gttgtagagg ctcactgcca ggagggccag aaccgcaatg
    13381 ataatcaaca atgcatagaa catttcactg gtttttattt gtttccaaaa ataacacatt
    13441 aataactatt ctgacaggtt ttcagagatg tgcctgtact tattttaagc ctctccatgt
    13501 cgcgtagtac cacttgtcag gctaactacc cgggtcttcg gaagataaac tcatgttaca
    13561 tgctccccgt attccattcc atgaagaacc gttcgaggta cctgaccatg taatcatgtc
    13621 tctcttcagc aagcctgcgg cccgtgggag tattcataag gtctttcagg agcaggagtt
    13681 tgtcatagaa gtgatgaatg gtcgacgccg attttgaacc ggttccggcc agcaggggcg
    13741 ggtcagctat gcttatgcgg ggatcatata tttcattccc actgaacccc ccgtaactga
    13801 atgccctggc aatgccgata gctccgatgg cgtcgagcct gtcagcatcc tgaactatcc
    13861 ggaactctat gcctgcgctg tcaggtcttc tgccttttga gaatgagata ttctcattga
    13921 ttctgacaac ttcatctgtt accttgctgt ctacgccaag gcgctccagc agccttcgta
    13981 tctctgcagg cccgtcgtga gccctgaact tatggtcgcc cacatcgtgc aaaagagcgc
    14041 ccagttctac tgtcagcctc tctccacggc cctcactttc acggatatgc agggccattc
    14101 tgacaaccct ttcgatgtga gaccatccgt ggccgctttc atgctccagc atagcctcct
    14161 tcacgaagct gattacctca ccgatgactg acttctcatc tttcataagc aacaaaataa
    14221 ttagtgactc aaaatattga taattttgcc acgctgacca aatctcacaa acaagatatg
    14281 aacagcgcag agattatcac gataggcgat gaattgctga taggacagac cgttgacacc
    14341 aactcggcat ggatgggcat gcagctcagt atgaggggta tacaggttaa ccggattgtc
    14401 tcgatctctg acaggaggga agagatcatt gcagctctcg atgagtcgct gcgcagggtg
    14461 ccgcttgttc tgattacggg aggactcggg cctaccagtg atgatatcac gaaggagaca
    14521 ctgacagagt atttcgggac gaagctggta atgagccagg aagttcttga tgaagtcacc
    14581 aacaggatca cgcggaggaa cctggagatg aatgagaaca acaggcgtca ggcgatggtg
    14641 cctgagagct gcagggtact tgcgaaccac gcgggcacgg ctccggccat gctgtttgaa
    14701 aaggagggaa ggatcattgt gtcaatgccc ggggtgccct cggagatgaa gcacatcatg
    14761 catgaacatg ttctgccgat gatatcttcc atgactggcg gtcataccat cattcacagg
    14821 aacataatga ctttcggcac tttcgaggcg aagctcgccg agaggctgga gggttttgaa
    14881 aaggaactgc ctgccatggt gaagcttgct tacctgcctt cacacggtgt catcaagctc
    14941 cggctgaccg gcagcggaga tgaccctgaa ctggtgcgca ggacggagga ggagcaggta
    15001 cggaaactat actccatcat acctgatgtt atctacggtg aagatgaaat aacgcttgag
    15061 gagattgtag gcaagttatt gcttgacaat aatcagacag tatcaactgc tgagagctgt
    15121 accggcggga agattgcttc gatgatcacc tcagtgcccg gcagttctgc ctggtacagg
    15181 ggatcagtgg tagcttatga caacagcatc aaggccgggg tgctggggat tgaccctgac
    15241 atcataagca ggtacggtgc ggtaagtgag gagacggtga gggccatggc tgagcgtatg
    15301 cggtcaatag cagaaactga ctatgctgtt gcagtgacgg gtatagccgg acccaccgga
    15361 gggactcctg aaaaacctgt cgggaccgtc tggattgcag tcgcctcagc agcaggcgta
    15421 gtagctgaga aacacctgtt tgcagatgac cggcagatca acattttcag gtctgcaacc
    15481 aacgccctca acctgctcag aaagcatata atcagcaggt aaccctcgtg aaagcggctc
    15541 tgccggaaaa aaatgcataa taaatttggt taatagaata aatatcacga attttgcgaa
    15601 ccgatttttg gagttttcag aacaagtaaa aaggatagta atgtcaaagg tttgtcaggt
    15661 caccgggaag agagcgatgg ttggaaacaa tgtttcccac tcgaagagga ggaccaagag
    15721 aaaatttatg gtcaacctgt tcaaaaagag gtatttcctt gccgaggaga acaggtggat
    15781 cacccttaag gtatcagccg caggaatgag gaaaatcaac aagaacggac tttcatcggt
    15841 gcttaaggaa gcaaaggaaa aaggttacat tatcaactaa attaaaccgg aaaagagatg
    15901 gctaagaaga ccaaaggcaa cagggtgcag gttatccttg aatgcactga gcacaaggaa
    15961 tccgggcagc cgggcacctc aaggtatatt acaaccaaaa accgtaagaa cactcctgac
    16021 cgtattgaga gaaggaaata caatcccatc ctcaagaagg tcacagttca cagggagatt
    16081 aaataattca gtgagatatg gcaaagaaag ctgttgcaac acttaaggca ggaaccggca
    16141 aagcattcac caagtgcatc aaggtggtga aatcagagaa gaccggcgca tatcagttca
    16201 aggaagaagt agttaataac gaaaatatta atgacttttt caccaggaag taaggtcagt
    16261 tttttctgat cagttataca gggagacgcc ggtaagtgcg tctctttttt tacatttggg
    16321 gtattaacag cagaagaatg gcattgttcg gattgtttaa aaaggaagag aaggagatac
    16381 ttgaccgtgg cctgagcaag acacgtgaca gtgtcttttc gaagctcacc agggctgtgg
    16441 ccggcaaatc gaaaattgac gatgaggttc ttgacaacct tgaggaggta ctggtcactt
    16501 ccgatgtggg ggtggagaca accctccgta taattgacag ggtttcaaag cgcgttgcac
    16561 gtgacaagta cctgaacact actgagctca atgccatcct gcgggaagag attgccggtc
    16621 tgctcgaaga gaacaaggtg acgcatgatg tatcctcagg atttgatgca cctctgccgg
    16681 caaaaccata cgtgatcctc gttgttggcg tcaacggtgt gggcaagacc acaaccatcg
    16741 ccaaactggc atacaatttc aggaaagcgg gacgcagtgt gcttctcggg gcagccgata
    16801 ctttccgtgc tgccgcaata gaacagctta aaatatgggc agagcgtgcc ggagtgccga
    16861 tggtgagcca gcagatggga gccgaccctg cttcagtggc ctatgatacc ctgaagtcgg
    16921 cagtggcttc agaaactgat atagtactga ttgatactgc cggacgtctt cacaacaaga
    16981 ttggactgat gaacgagctg tcgaagatac ggagggtgat ggagaaggtg atacccttcg
    17041 cacctcatga agtgttgctc gtccttgacg gatcaacagg tcagaatgct tttgagcagg
    17101 ccaggcagtt cactgctgcc accgaagtca atgcactggc aatcaccaag ctggatggca
    17161 ctgcaaaagg cggggtggtg attgggattt cagaccagtt cagaataccg gtcaaataca
    17221 tcggggtggg tgagaagatg gatgacctga ggatcttcaa ccgctatgcc tttgtcgatt
    17281 cactcttcag cgaccgcacc tgatgaaacg taacagggtc aatattgtca ccctgggctg
    17341 ctcaaagaat accgttgact cacagaaact gatgcgccag cttgcggctg gcggatacag
    17401 tgtcacagcc gggtcagccg atcctgccga cattgtgatc atcaatacat gcgggttcat
    17461 caatgatgca aaggaagaga gtgtggatac catcatcagg tatgccgaag ccatccgcag
    17521 ggagggccat ggcaggctgt tcgtgatggg atgcctggtg gagaggtacc ggaatgagtt
    17581 gcaacatgag cttcctgagg tggacgggtg gttcggggtg aacaggccgc aggagattat
    17641 ctcacgactg ggcctcgctt acagggagga gctgacgggt gaaagagtta ttaccggacc
    17701 cgcacattac gcatacctca aggtgtcaga agggtgtgaa cgaacctgcg ccttctgtgc
    17761 aatacccgac atacgcggga gatacatctc ggccccgctg gaaaaactgg tggaggaggc
    17821 acgcctgctt gcatcacaag gtgttaagga actgatactt attgctcagg acctgactct
    17881 gtatggcact gacctgtatg gcaggcagat gcttcccgga cttgtccgga ggctggctga
    17941 gattgactct cttgaatgga tcaggctgca ctatctctac cctgcaggct tcccgatgga
    18001 gatacttgag atcatgcagg agaatccgaa gatttgcagg tatattgata tccctgtcca
    18061 gcatatttcc gacaaggttc tgaagatgat gcgccgcgag catgacagtg cgggaacgaa
    18121 gaggctgctg gctgatatcc gccggatatt gcctgacgca gcggtgcgca ccaccgtgat
    18181 tgccggtcat cccggcgaaa cggagaaaga gtacagggag ctgcttgaat tcatcggtga
    18241 gtacaggttt gacaggcttg gtgcattccg gtactcacat gaggagaaca ctcacggagc
    18301 gctgaattat aaggacacaa tccccgaaag gacgaaggag aggagggtgg ccggcataat
    18361 gaagctgcag caggagatat cggctgcacg gaatgcctcc atgaccggga aggttataaa
    18421 ggtgatcatc gacaggagag aaggtctgtt tcatgtgggg cgcagccagc atgactctcc
    18481 cgaagtggac caggagatac ttgtcagagc cgccggaaag attgagcccg gcaccttcgc
    18541 agatgtgaag ataaccgggt caaccgagta cgatctgtat gcggaactat tgcagtaaag
    18601 ttgacgacag atgccggcag ggcatcaggc cattgttaaa tcatgcagta atgcagacaa
    18661 caggtaccgg cagggcatca ggccattata aaaacatgcc ggcaacaata cctcagtgat
    18721 tcgtaaccag gcaaatcgca aattcgctta tcccttcacc tgattgcctt cagg
//
LOCUS       DAOPBK010000026        18757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3053_length_18757_cov_5.531654, whole genome shotgun sequence.
ACCESSION   DAOPBK010000026 DAOPBK010000000
VERSION     DAOPBK010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18757
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_18757_cov_5.531654"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLA17_02680"
     CDS             complement(<1..418)
                     /locus_tag="PLA17_02680"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPK84347.1"
                     /translation="MRRAVSLILLLALGFAVSGQPLSSKKIDRLSARVIKEFNVPGVA
                     VAVVKDGEVIHMKGYGVRSIATGQKTDEHTLFAIASNSKAFTAATLGILVDEGKLRWD
                     TRVTEIIPEFRLYNPYVTEEFIIKDLLCHRSGMGEGA"
     gene            complement(415..1977)
                     /locus_tag="PLA17_02685"
     CDS             complement(415..1977)
                     /locus_tag="PLA17_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPK84348.1"
                     /translation="MKKILAFSLMLLTCIATWSQPLKSDEIDRLVNRTLETFNVPGIA
                     VAVIKDDQVVHMKGYGVISITTGKKTDENTLFAIASNSKAFTSAALGILVDEGKITWD
                     TKVIDIIPEFRLYNSYVTEDFNIKDLLTHRSGMGLGAGDLMLWPDSSTFTREDIIHNL
                     RYLKQTSSFRTKYDYDNLLYIVAGEIVRRVSGQGWDEFVESRIMQPLGMTRSAASLNR
                     VRDRSNMIDAHVPVEGVLQVVPMYANTLLDPAGGIMSSVADMSKWVMMQLNRGKYGDG
                     NSRQMLSETVHREMWTPQTIMRLRNPGPYRSHFAAYGLGWSLTDVAGYLQASHTGGLA
                     GIVTQVTLIPELKLGIIVFTNQQEGSAFTAITNTIKDGYLDVKGNDWVKTLRENVDRN
                     LNEAKRITDEIWAKIEAQRSEGADQVSNAVYTGTYTDAWFGDIIISDEGGKLRFQSVR
                     SPKMCGEMFYYTGNTFVVKWDDRSMDADAWAVFALDKEGRAEAFTMEAISPLTDFSYD
                     FHDLSPKRKEKR"
     gene            complement(2017..4113)
                     /locus_tag="PLA17_02690"
     CDS             complement(2017..4113)
                     /locus_tag="PLA17_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPK84349.1"
                     /translation="MKQLTNLTALIMLLLILPGCGDRKSKSAEATDPWLWLEEVDAEK
                     SLDWAREQNRLTDEVLTVRPEFTELRDRFLGVLNDKERIIYPNITGEMSYNLWQDEKN
                     ERGLWRRMSKKDYLAGKDVWETVLDLDLLSAEEGKKWVFGGASWLGPENRYCLLWLSD
                     GGKDESYIREFDAVEKKFVEGGFSIPESKGSAAWVDRDQILVATNYGPGTMTSSGYPA
                     MAKLWKRGEDPANAVTMHSTDTANMGIFVSGGEVNGKQYVFLSDNIGFYESSTYLLTP
                     EGMKELDIPKDVETRGMFREAMLLYLQSDWTLNGTTYKKGTLVSFDLPAFLAGSPVVK
                     TLYEPGERESLGGVMSSRDFIVIATLENVQGRLRIMRPDGESWVTEALPVPEFGTVGL
                     ADADDKSNDFFFTFSNPVTPSTLYYGDGKQIRPIRKQKEYFNATGVKVDQYEVTSKDG
                     TKIPYFIVYPAEMALDGSNPTLIYAYGGFNSAVRPGYSSITGTGWIEKGGVYVIANIR
                     GGGEFGPEWHHSALKEKRQNAYDDLFAVAEDLISRKVTSPERMGFYGWSNGGLLAGVV
                     MTQRPDLWNAVVIGAPLLDMKRYNKLLAGASWMDEYGNPDIPGEWEFISKYSPYQNVK
                     KDVKYPEPLFITSTRDDRVHPGHARKMAAKMIDMGHPVLYHETIEGGHGAASTNAQEA
                     EMWALMYTYLNMKLAK"
     gene            4686..5018
                     /locus_tag="PLA17_02695"
     CDS             4686..5018
                     /locus_tag="PLA17_02695"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HPK84350.1"
                     /translation="MNSRNAICFRLILALLTAMIFACFTACSPDSATENPSDQKIILY
                     AEFMHEVNSFSPVITTEKNFKADHLFYGDQVPASAIEEDKQLAGFLSAVDKSGRGRIK
                     AIKTSKNI"
     gene            5020..7524
                     /locus_tag="PLA17_02700"
     CDS             5020..7524
                     /locus_tag="PLA17_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPK84351.1"
                     /translation="MKKITGLLFLLLITGVTTAQNKTVCQDWRISGVTRLFMPFFADQ
                     PSVDGKTFDAAALLENVQTDDADLKSWPVVTIPGDSVLTSVNSENQMVRMAGFISSDR
                     WTKASINISTNAIFELSLNGKRVKSQGKPSDRPVKIDLTLGTGIHEMSLKLISMEKTL
                     RFAADLSAANDSAVLSWSTNPTRTLTINDVLEGVSISGARLSNSGKYLLIQTNEVLAG
                     SGKIQSSFQVYDMELKRNLFALRNNALRADWMPKSDRLYYQVNRNETSDVIIYDMKSG
                     TESVAASGLKSAGRIVWSPAEDYFVYSRNEEAEKTGDLKRVFDNEDRIPGTRNRSYLY
                     MYNPGTKVSQPLTAGSRSAMLQAISPDGRRVLFSVSRQDYSEPPFSKQTLYELEISTM
                     RLDTIWSDKLHGGSCQYSPDGTQLLVSGGPETFGSLGVKVSEGRIPNSYDTQLFLFDL
                     KSRKAEPLTRNFDPAAGMAYWAPDGKIYLTATERDYVNLYRLDPKRRSFTRIDVPVEV
                     ITTVDYAKNNSIAVLRGTSICIPDRLYTLDLSTGKSTLLCDPAAGRLEDVISCQTEEW
                     NFKNSRGTTIYGRVYYPVNYDPSKMYPLIVNFYGGTTPTSRAFGGRYPKEIWAAEGYM
                     VYVLQPSGATGFGQDFSALHVNGWGREAIDDIIEGTKKFLAAFPSADADNVGCIGASY
                     GGFTTMMLQTRTDIFRTAIAHAGISDITSYWGEGYWGYSYSAGATTGSYPWNRRDIYV
                     DNSPLFNADKFSNSILLLHGTADTNVPPGESLQYYAALKILGKDVEMVLVSGENHQIL
                     DYKKRIEWHNTIMSWFDMKLKNQPQHWNEMYPDKNL"
     assembly_gap    7556..7655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7656..7954)
                     /locus_tag="PLA17_02705"
     CDS             complement(<7656..7954)
                     /locus_tag="PLA17_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84352.1"
                     /translation="MDKVIFFIALSLPVIFFSKKSLFEFHSQGFTRFFSFECIILLFV
                     FNYTKWFSEPFSLKQIISWLMLLVSLWFLIEAVIRLRRARKPGIVKVDEKLFRFG"
     gene            complement(7942..10302)
                     /locus_tag="PLA17_02710"
     CDS             complement(7942..10302)
                     /locus_tag="PLA17_02710"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPK84353.1"
                     /translation="MKKSLVIILLVQILFSGCKNDNRSSNDYILWYDRPAAEWIEALP
                     VGNGRLGGMLFGDPSFGRLQVNEESLWGGVNVPNNNPGALANLPGIRELILDGKIPEA
                     YELSEKYLAGIPGTTWSYQTVGDIFFRFADTTGVTGYRRELDLKTGIAGVSFDRNGKK
                     IEYEVYVPAGDNLMVISMRSQEVGGLDMDIMLTRPQDAVITVSENRIDMEGQIIDPDD
                     PDKGPGGEHMKFFASLIVMSCDGELKAGEKSLNYTGGSEMTLFFDARTDYNIDLLNFD
                     RTIDPRKDCVEGISKASWKPLTKIRKDHISEHSSMFNRVQLDLGGDPALEELPTDERL
                     RKFQAGGDDPGLIATYFQYGRYLLMGSSRHPAQLPANLQGIWNKEISAPWNSDYHTNI
                     NLQMNYWPAEVCNLTETSRPLVRFMEELTIPGSITARETYGARGWTLHHTTDVFGKTA
                     VLDGVYWGMFPLGGAWMTLPLWEHYEFTNDTAYLREQAYPVMKGAAEFIVTFLVPDRQ
                     GRLVTVPSYSPENSYIDPVSGKEFKLTYAPSMDNQIIREVLLNCRKAATILGIDREWS
                     DSLQTILDRVPADRVASNGTISEWIEEYEEAEPGHRHMSHLFALHPGTQITPETPELY
                     AAARRTLERRLENGGGHTGWSRAWIINFYARLLDGDEAYKHLRLLLEKSTLPNLFDNH
                     PPFQIDGNFGGTAGIAEMLLQSHNGVVHLLPALPAAWPDGKVTGLKARGNFETGMEWH
                     GGVAEIITVKSLSGGQLNLSYNGKLHSMVTEKGVTYKFDSNLQWIK"
     gene            complement(10448..11362)
                     /locus_tag="PLA17_02715"
     CDS             complement(10448..11362)
                     /locus_tag="PLA17_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84354.1"
                     /translation="MKRSRFTMAGIKRYLLVAVIMLVAVILLANKLIVWGAVFIAAGL
                     IVLGIQQLIQLNVKVSKFEDSISSLREKNQDLAKINEKLTEENAFLKDRKFQITQIKS
                     ILEMNLFEIDTRFKRTVNRREMLHDREIRHIGSLGVSLKAKYGIDLKELRFKYVPESD
                     ELIVANIHPRFLSFGNRKLEWEFFETLEYKRQLPLTVKRWMTSDDLAEKADRLKEEIR
                     LETEQSIENGPEEFSWIMEPIRENVENTIRIMFRGICNNIRIAELADSSFVPLEGIRL
                     EKPESQDSGHSLKAPGGINSGMKKYLDE"
     gene            complement(11359..>12084)
                     /locus_tag="PLA17_02720"
     CDS             complement(11359..>12084)
                     /locus_tag="PLA17_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HPK84355.1"
                     /translation="RAGYYCFDTFTPLNQEAYLAARWGVNCVLSAADEMLAGTRYAYV
                     LTRPPGHHTERSVFGGFCYFNNCAIAAHYLSRSSRVAILDIDYHHGNGQQQIFYSRSD
                     VLTISIHGHPSFAYPYFSGFEEEKGEGDGIGFNHNFPLPEKATHEQYVKALSRALNII
                     KKYNPDYLIVALGLDPAKGDPTGTWSFTHSNFTTNGEMIGSLKLPTLVVQEGGYRNQS
                     LGINARAFFTGFHRAHNSKNPTT"
     assembly_gap    12085..12184
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12286..12867)
                     /locus_tag="PLA17_02725"
     CDS             complement(12286..12867)
                     /locus_tag="PLA17_02725"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPK84356.1"
                     /translation="MSYTLQPGQKAIDFRLPATDGREYSLSDFNDHPFLVIFFTCNHC
                     PYVTGSDEVTRATAEKFREKGVAFIGINSNSEHTYEEDSFPNMIKRMKEHNFPWKYLH
                     DREQTVVKDYGALKTPHFFVFNRDRVLVYTGRAVDSPRDASRITVNDLDRVLEELTSN
                     RQISVPVTNPIGCNIKWEGRDAKWMPPEACDLV"
     gene            complement(12975..14102)
                     /locus_tag="PLA17_02730"
     CDS             complement(12975..14102)
                     /locus_tag="PLA17_02730"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPK84357.1"
                     /translation="MRTALLLSVCLLAVSCSTKQTGKTMFTSLDSLLASEFAAGEPGG
                     ALLVMKGDSIAYSKSFGIADIVTGEKITENTIFNLGSISKTFVANGILILQEEGKLSV
                     DDPLSKYFPEFKSTEIADRVTIMHLLTHTSGLPDNRPVSENPEFFLTAKDYENWAPVT
                     QAEDLVFEPGSRYEYSNPAYNALALIIEKVSGQKWQDFIAERIFKPAGMATSTITDGP
                     HPETGVSHAYEMVNGVWTESDYGEFPTFAAAGNGGVWSSVTGLALYEKAIRENLFLGA
                     ELTNKSRTICRPDAWAGDEPPFIGYSWFIGEEQILGQANKFGVKFVYHTGDQGGFKAF
                     HFSVPEKDLLYVGLFNRPPESLNRIIIKGLSILKENDWLDR"
     gene            complement(14189..15355)
                     /locus_tag="PLA17_02735"
     CDS             complement(14189..15355)
                     /locus_tag="PLA17_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPK84358.1"
                     /translation="MMNNRQIISTVAAILLMLPVLNHHNTYAQMKRARDYGIEIGILR
                     TGSNNAITDVPGVKVGQVTLWEGDSVRTGVTAILPHEGNIFQQKVPAGIFVGNGFGKL
                     AGYTQVEELGNLETPIVLTNTLNVATAVAAVIEYTLSQPGNERVMSVNAVVGETSDAR
                     INDIRGRHVTMEHVLEAIRNAAGGPVAEGNVGAGAGTVCFGFKGGIGTSSRVLPAVRG
                     GYTVGVLVQTNYGGILQINGVPVGQELGRYSFKNTVNEYKEDGSCMIVVMTDAPLTSR
                     NLKRLASRAFAGMMRTGSSGSNGSGDYIIAVSTAEELRIPYSSDSMYEQWREIRNDEM
                     DLLFQAAAEATEEAIINSLFAAESTRARNGMIIEALPVEKTLEIMRKYNRLTEK"
     gene            complement(15368..17032)
                     /locus_tag="PLA17_02740"
     CDS             complement(15368..17032)
                     /locus_tag="PLA17_02740"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPK84359.1"
                     /translation="MKRALFPAAIILLLVAISCNNSTQTSDMIITNARIWTGNADQPF
                     AEAMAVSGDTIVAIGSNREVMKHRSVSGTLIDLGGMFVVPGFIDTHVHLLQGGSNLAS
                     VQLRDASTPEEFIKRIREFAATQKPGTWILGGDWDGMGWESLPARSWIDSVTSDHPVF
                     VSRLDGHMALANSLAMKIAGIDRKVKDVTGGTIERDARGELTGIFKDNAMDLISVKIP
                     EQSAEEADLALVAAMKYLASNGVTSVHALDAAAYADGLDRVRASGRQITRIYYLQTIS
                     RWADMKEQVERTGRGDRWLSTGGVKGFVDGSLGSHTAAFLEPYTDLPTDTGLFVNSEQ
                     DLYTWVSAADKAGLQVAIHAIGDRAINTLLNIYERVADENGDRDRRFRIEHSQHIAPA
                     DIERYGKLKVIASMQPYHAIDDGRWAEEYIGPERAKTTYAFRSLLDSGATLAFGSDWF
                     VAPATPLEGIYAAATRRTLDGMNPGGWIPEQKITVEEALKAYTINAAYASFEEDFKGT
                     LEPGKLADFVVLDRDITAIDPVEIYDMKVQQTWVGGKKVFDRTEEQ"
     gene            complement(17045..17437)
                     /locus_tag="PLA17_02745"
     CDS             complement(17045..17437)
                     /locus_tag="PLA17_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPK84360.1"
                     /translation="MELKTDHTKQLYSYLSLRKAVGWTGILLSLGVSCLTRGGTLYPD
                     KPTKKHIMENEEKLIRVFTGPEPTTLLLMEMLEETGIKSLIKNDSGLGYLGAVPPVMD
                     LYILEENLEKAIPLLNEFKEKHYPETDS"
     gene            complement(17630..17926)
                     /locus_tag="PLA17_02750"
     CDS             complement(17630..17926)
                     /locus_tag="PLA17_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84361.1"
                     /translation="MRLTLNRYAGPMRKSIGRTLRVTMLITVAFIIKVLNSCIHPVRD
                     FDCSFNRITIRNLDNSGIYAPETDRDIMYTSAVAIIFAAVIHPVQRVNHSFNTC"
     gene            complement(17939..18412)
                     /locus_tag="PLA17_02755"
     CDS             complement(17939..18412)
                     /locus_tag="PLA17_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK84362.1"
                     /translation="MKVNIRRANEEDFPAILALINELAEYERAPEAVTNTVEQMGRER
                     DYFRCFVAESADHGIVGMALYFFAYFTWVGKSLYLEDIIISKQFRKRGIGEALMKRVM
                     REAWEENCKRVRWQVLDWNEPAISFYRKSGAEINNEWLNCTFDEKGIALFNELNS"
     gene            complement(18446..>18757)
                     /locus_tag="PLA17_02760"
     CDS             complement(18446..>18757)
                     /locus_tag="PLA17_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPK84363.1"
                     /translation="AKSSNFYLRFKGEVEEAVALMGIPSVSVFRPSLLMGKRNEFRLA
                     EEISKIFTPLLSFLFPAKYKPIKGLNVAQAMIAAASIEEPGFRVYHFNEMKALIKGSK
                     A"
BASE COUNT         4299 a         5087 c         4399 g         4772 t
ORIGIN      
        1 cagcgccttc acccatcccg ctccggtgac acagcagatc ctttatgatg aactcttcgg
       61 ttacatacgg gttgtaaagc ctgaattcag ggattatctc agtcaccctg gtatcccatc
      121 tgagtttgcc ctcatcaacg aggataccca gcgtggcagc agtaaacgcc ttgctgtttg
      181 atgctatcgc gaagagggta tgttcatcag ttttctgacc ggtggcaatt gaacgaacac
      241 catatccctt catgtgtatc acctctccgt cctttaccac agccacggcc acccccggga
      301 cattaaactc ctttatcact ctggcgctca gcctgtcaat ctttttagac gacagaggct
      361 gacccgatac tgcgaagcca agtgccagga gcaggatcag tgaaactgct ctcctcatct
      421 cttctccttt ctcttcggac tgaggtcatg gaagtcatag ctgaagtcag tcagcggtga
      481 gattgcctcc atggtgaatg cttcagccct gccctcctta tcgagcgcga agaccgccca
      541 agcatcggca tccatagacc tgtcatccca tttcacgaca aaggtgttgc ctgtgtaata
      601 gaacatttct ccgcacattt ttggtgacct gaccgactgg aaacgcagtt ttcctccctc
      661 atccgatata attatgtctc cgaaccacgc atcggtataa gtgccggtat aaacagcatt
      721 gcttacctga tcagcaccct ctgagcgctg tgcctcaatt tttgcccata tctcgtcggt
      781 tatccttttt gcttcattga ggttccggtc aacattttcc ctgagggttt ttacccagtc
      841 gttccccttt acatccagat aaccatcctt gatggtgttg gttatggcag tgaaagcaga
      901 gccttcctgc tggttagtga agactattat tcccagcttc agctccggta taagggtaac
      961 ctgggtaaca atgcctgcca gtcctccggt atgtgatgcc tgcaggtatc ctgccacatc
     1021 ggtaagactc cagccaaggc cgtaggcggc aaaatgactc ctgtacggcc cgggattgcg
     1081 gagccgcata atggtctgcg gcgtccacat ctcacgatgc accgtctcag agagcatctg
     1141 cctgctgttg ccgtcgccgt attttccacg gttgagctgc atcatcaccc atttgctcat
     1201 gtcagccacg ctgctcatga taccgccggc agggtcaaga agagtgttgg catacattgg
     1261 tactacctgg agaacacctt ccacgggaac gtgcgcatca atcatattcg acctgtctct
     1321 caccctgttg agcgaggctg cagatcttgt cattccaagc ggttgcatga tccttgactc
     1381 cacaaattca tcccatccct ggccggagac cctcctgaca atttcgccgg caacaatgta
     1441 aagcaggtta tcataatcat attttgtcct gaatgatgag gtctgtttca gatatctcag
     1501 gttatggata atatcctccc tggtgaacgt ggatgaatcg ggccagagca tcaggtcacc
     1561 tgcaccaaga cccatcccgc tccggtgggt cagcaggtcc tttatattga aatcctcggt
     1621 aacgtaggaa ttataaagcc tgaactccgg tataatgtca atgacctttg tatcccaggt
     1681 tatcttgcct tcgtcaacca gtattccgag agcggctgag gtgaatgctt tcgaattgga
     1741 tgcaattgca aaaagggtgt tttcgtcagt ctttttgccg gtggtaatgg agattacacc
     1801 gtaccccttc atgtggacta cctggtcatc ttttattact gccacggcaa tgcccggtac
     1861 gttgaaggtc tccagcgtac ggttgacaag cctgtcgatc tcgtcagact tcaggggctg
     1921 tgaccatgtt gcgatgcagg tcagcagcat cagtgaaaat gccagaatct ttttcatgtc
     1981 aacggtttaa gtcaatgaga atatgccttc aggcccttat tttgccagct tcatattcag
     2041 gtaggtgtac atcagtgccc acatctcagc ctcctgagca ttggttgatg ctgctccatg
     2101 gccgccctca atggtctcgt gataaagtac aggatgtccc atatcaatca tctttgccgc
     2161 catcttgcgg gcatggcccg gatgcaccct gtcatccctg gttgaagtaa tgaacagggg
     2221 ttccgggtat ttcacatcct tctttacatt ctggtagggc gagtatttgc tgatgaactc
     2281 ccactccccg ggaatgtcag ggttgccgta ttcgtccatc cagcttgcgc cggcgagaag
     2341 cttgttgtac cgcttcatgt caagcagcgg agccccgatg acaactgcat tccagagatc
     2401 aggcctctgt gtcatgacca cacctgccag caatccgccg ttgctccagc cgtagaatcc
     2461 catcctctcc ggcgaggtta cctttcttga gatgagatct tcagccacgg caaagagatc
     2521 atcatacgca ttctgcctct tctccttcag agccgaatgg tgccattcag gaccaaactc
     2581 gcctcctccg cgtatatttg caatgacata gacgccaccc ttttcaatcc agccggtacc
     2641 ggtgatggaa ctgtaacccg gacggacagc cgagttgaaa ccgccatagg catagatcag
     2701 ggtcggattg ctgccgtcca gggccatctc tgccggatag acaatgaaat agggaatctt
     2761 cgtcccatcc tttgatgtca cctcatactg gtcaaccttc acacctgtgg cattgaaata
     2821 ttccttctgc ttcctgatcg gcctgatctg cttcccgtct ccgtaataga gggttgaggg
     2881 agtgaccgga ttgctgaagg tgaagaagaa gtcatttgac ttgtcatccg catctgcaag
     2941 gccgacagtg ccgaattccg gtaccgggag agcctctgtg acccagctct ctccgtcagg
     3001 acgcattatc ctcagtctcc cctgaacgtt ttccagcgtt gctatcacga tgaaatcacg
     3061 ggatgacatc acacctccaa gactctccct ctctccgggc tcgtaaagtg tttttaccac
     3121 aggactccct gcaaggaacg cagggagatc aaaactgacc agcgtaccct tcttgtatgt
     3181 tgtgccgttg agagtccagt cagactgcag gtaaagcaac atcgcctccc tgaacatccc
     3241 ccttgtctca acatccttcg ggatgtcaag ttccttcatc ccctcagggg taagaagata
     3301 agtcgacgac tcatagaaac cgatattgtc actcagaaaa acatactgct ttccgttaac
     3361 ctcgcctccg ctgacaaaaa tacccatgtt ggcggtgtca gtactgtgca tggtaactgc
     3421 atttgccgga tcctcacctc tcttccacag ttttgccatt gccgggtatc ccgatgaggt
     3481 cattgttccg ggaccgtaat tggtggctac aagtatctga tccctgtcaa cccaggctgc
     3541 actgcctttg ctttcaggaa tggagaatcc tccctcgacg aactttttct ccactgcatc
     3601 aaactcgcgg atataactct catccttgcc accgtcagag agccagagca gacagtaacg
     3661 gttttccggc ccgagccatg aggctccgcc aaagacccac tttttccctt cttcagccga
     3721 aagcagatcc agatcgagaa ccgtctccca cacatccttc cctgccaggt aatccttttt
     3781 tgacatcctt ctccacaggc ctctctcatt cttctcgtcc tgccagaggt tatagctcat
     3841 ctctcctgta atgttagggt agataatcct ctccttgtca ttcagcaccc cgaggaacct
     3901 gtcgcgcagc tccgtgaatt ccggtctcac cgtcagaacc tcatccgtga gcctgttctg
     3961 ttcacgggcc cagtcaaggg acttttccgc atcaacctcc tcaagccata accaggggtc
     4021 agtggcctct gcacttttac tctttctgtc tccgcaaccg ggtaaaatca gtaacagcat
     4081 aatcaatgcc gtcaggttag ttaactgttt cattttttga atatttatga tttcttgtta
     4141 atgtatttaa tgttgcatct gaaaatgagc gcttccccga taataaagag aggccatctg
     4201 taatgatcag gaacacaagt tgttctcttc attgtcaacc gttaacctgc ctccggcgat
     4261 ttcttccgta aaaaacggct tttccagctt ggatatgatc ctttcatggt attcctgttt
     4321 gctgcggcaa attacaaaat ataagggaga actcttttcc tccccgccgg ataattggga
     4381 aaatggctcc tctgccatca gggaacgtgc cttggtctgg ccagtacttg ccggcaaacg
     4441 gttgaccggt gacacagcca tgtgcggggg aatgtaccct gatctgacca gtgtgctccg
     4501 gcaggcgctc aaccggtgac gcagccatgt gcgggggaat gtaccctgat ctgaccagcg
     4561 ggtcacccgg ttgcgttcat gacagctgcc ggaatcgcat tgacagatgg ctcaatgatc
     4621 aacaactaca acggaactta atattttaaa tccctttttt tatatttgtt gaaataccgt
     4681 ctataatgaa ttccagaaat gcaatttgct tcaggctgat ccttgcgctt ctcaccgcaa
     4741 tgatatttgc ctgtttcacg gcctgttctc ctgacagtgc aacagagaac ccttcggatc
     4801 agaagattat actttatgct gaatttatgc atgaggtgaa cagcttctcc ccggtgatta
     4861 caacagagaa gaatttcaag gcagatcatc tcttctatgg cgaccaggtg cctgcctcgg
     4921 ccatagagga ggacaagcag cttgccggat tcctctcggc agttgacaaa agcggcagag
     4981 gaagaatcaa agccataaaa accagtaaaa acatataata tgaaaaaaat cacgggcctt
     5041 ttattcctgt tgctgattac aggagtcaca actgcacaaa acaagactgt atgtcaggac
     5101 tggagaatct ccggagtaac ccggctgttt atgccgttct ttgctgatca gccatcggtt
     5161 gacggtaaaa cttttgatgc ggcagccctt cttgaaaatg tacaaactga tgatgcagac
     5221 cttaaatcat ggcctgttgt aacaattccg ggcgacagcg tgctcacttc agtcaacagt
     5281 gaaaaccaga tggtcagaat ggctggtttt atcagcagcg accggtggac caaagcctcg
     5341 atcaatattt caaccaatgc catcttcgag ctcagtctca acggtaaaag ggtgaagtca
     5401 cagggcaagc catcagaccg gcctgttaag attgatctga cactgggaac aggaattcat
     5461 gagatgagcc tgaagctcat cagtatggaa aaaacgctca ggttcgccgc tgacctgtca
     5521 gcagcaaatg attcggcggt actgtcatgg agcacgaacc ccacacggac acttacaata
     5581 aacgatgtcc ttgaaggagt gagcatatca ggagcaaggc tttccaattc aggcaaatat
     5641 cttcttatcc agaccaatga ggttttggcg gggagcggga aaatccaaag ctccttccag
     5701 gtttatgata tggagctgaa aaggaacctt ttcgccctgc gaaacaatgc cctccgggca
     5761 gactggatgc caaaatccga ccgcctctat taccaggtta acaggaatga gacgagcgat
     5821 gtgatcatat atgacatgaa gagcggaaca gagagcgttg ccgcatccgg actgaaatcg
     5881 gccggcagaa ttgtctggtc tcctgcagag gattattttg tttactcacg gaatgaggag
     5941 gcagagaaaa caggtgatct gaaacgggtt tttgacaacg aagaccgtat tcccggaacg
     6001 cgcaaccgga gttacttgta tatgtataac ccgggcacga aagtgtcaca accccttacc
     6061 gccggatcaa ggtcagcaat gctgcaggca atcagccctg acggccggag ggttctcttc
     6121 tccgtttcac gccaggacta cagcgagcct cctttctcca aacagaccct ctatgaactg
     6181 gaaataagca cgatgcgcct cgatacaatc tggagcgaca aactgcacgg cggttcatgc
     6241 cagtactcgc ctgacggaac acaactgctg gtatcaggcg gaccggagac tttcggcagc
     6301 ctgggggtga aggtgagcga gggcaggatt ccaaacagct atgatacgca gctctttctt
     6361 tttgatctga agagcaggaa agcagagcct ctgacacgca attttgaccc tgcggcaggg
     6421 atggcatact gggctccgga cggcaagata taccttacag ctacggaacg tgactacgtg
     6481 aatctctaca ggcttgaccc gaaaagacgg agctttacaa gaatagatgt gccggtggag
     6541 gtcatcacta ccgttgacta tgcgaaaaac aacagtattg ccgtgcttcg cggaacaagc
     6601 atatgcattc cggacagact atatacactt gacctgtcaa ccgggaaatc aaccttactc
     6661 tgtgatccgg ctgctggccg gctcgaagat gtgatatcct gccagactga agaatggaat
     6721 ttcaaaaact cccggggtac aaccatctat ggaagggttt actacccggt aaattatgac
     6781 ccgtcaaaaa tgtaccctct cattgtaaac ttctatggcg ggactacacc gaccagcaga
     6841 gctttcgggg gccgttatcc gaaggaaata tgggccgctg aaggatatat ggtttatgtc
     6901 ctgcagccct ccggagcaac gggattcggg caggatttct cagccctgca cgtaaatggc
     6961 tgggggcgtg aggcaattga tgatataatt gaaggcacaa aaaagttcct ggcagcattc
     7021 ccgtctgccg atgctgacaa tgtgggatgt atcggcgctt cgtatggagg gttcaccacg
     7081 atgatgctgc agacacgtac agatattttc cggactgcga ttgcccatgc cggcatcagt
     7141 gatattacca gctactgggg tgagggctac tggggctatt cttacagcgc gggcgccaca
     7201 acaggcagct atccctggaa cagacgggat atatacgtgg ataacagccc gttattcaac
     7261 gctgacaagt tcagtaactc catactgctc ctccatggaa cggcagatac caacgtgccg
     7321 ccaggtgaga gcctgcagta ttatgcagcc ctcaaaatcc tgggcaagga tgtggagatg
     7381 gtactcgtca gtggtgagaa ccatcagatt cttgactata aaaaacgtat tgagtggcat
     7441 aatacaatca tgtcatggtt tgacatgaaa ctcaagaacc agcctcagca ctggaacgag
     7501 atgtatcctg acaagaacct ctgaccggga aatgccgggc aatcagcggc agaggnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgccc gaacctgaac agcttctcgt
     7681 cgaccttcac aatacccggc ttcctggccc gccttaacct tataaccgcc tcaatcaaaa
     7741 accacagtga gacgagaagc ataagccatg agatgatctg cttcaaagaa aaaggctcac
     7801 tgaaccactt cgtatagttg aaaacaaaaa ggagaattat gcactcaaag ctgaaaaacc
     7861 ttgtaaaccc ttgactgtga aactcaaaca gactcttctt tgaaaaaaag atgaccggca
     7921 ggctcagggc aatgaagaaa attactttat ccattgcagg ttactgtcga acttgtatgt
     7981 aacccctttt tcggtaacca ttgaatgaag ctttccgtta taggacagat tcagctgacc
     8041 gcctgacagt gacttcactg ttattatttc agccactccg ccgtgccact ccatacctgt
     8101 ctcaaaattg cccctggcct tgagtcctgt taccttcccg tcaggccatg ctgccggcaa
     8161 tgcaggcaac aggtgcacca ccccgttgtg gctctgaaga agcatctccg cgattcctgc
     8221 agtacccccg aagttaccgt cgatctggaa tggcgggtgg ttgtcaaaga gattcgggag
     8281 cgttgatttc tcaagcagca gcctgaggtg tttgtaagcc tcgtcgccat ccaggagcct
     8341 cgcatagaag ttgattatcc acgccctgct ccatccggta tgcccgccgc cgttttcaag
     8401 ccgcctctcg agggtccgcc tggcagctgc atagagctca ggagtctccg gcgttatctg
     8461 cgttccggga tgcagtgcaa agagatgtga catgtgcctg tgccccggct ctgcctcctc
     8521 gtactcttcg atccactcgg atattgtgcc gttcgatgcc accctgtctg caggaactct
     8581 gtcaaggatg gtctgcagcg aatcggacca ctcacggtct atgccaagaa tggtggcagc
     8641 tttccggcag tttaagagaa cctccctgat tatctggttg tccattgacg gtgcataggt
     8701 cagcttgaac tctttgcctg aaacgggatc gatgtaactg ttctcgggag agtaggaggg
     8761 aacggtgaca agcctgccct gcctgtcagg caccaggaac gtcactatga actcagcggc
     8821 gcccttcatg acgggatatg cctgctcacg gagatatgcc gtatcatttg taaactcata
     8881 gtgctcccac agaggcagtg tcatccaggc gcctcccagg gggaacatac cccagtaaac
     8941 cccgtcaagc actgcggtct tgccgaagac gtcggttgtg tgatgcaaag tccatcccct
     9001 ggcaccgtag gtttctcgcg cagttatgct tcccggaatg gtcagctcct ccatgaacct
     9061 cacaagcgga cggctggtct cagtaaggtt gcaaacctcc gccggccagt aattcatctg
     9121 aaggtttatg ttggtgtgat aatctgaatt ccatggtgca gaaatctcct tgttccatat
     9181 accctgcaga tttgcaggga gttgcgccgg gtggcgcgat gaccccatga gcaggtaacg
     9241 tccatactgg aaataggtgg ctatcagccc cggatcatct ccgcccgcct gaaacttccg
     9301 gagacgttca tccgttggca gctcttcaag ggccggatca cctcccagat caagctgaac
     9361 cctgttgaac atcgacgaat gctcactgat gtggtccttg cgtatctttg taagcggttt
     9421 ccacgatgcc ttgctgatgc cttcgacaca atccttccgg ggatcaatgg ttctgtcaaa
     9481 gttcagcaga tcaatgttat aatcggtgcg tgcatcgaaa aagagggtca tctcacttcc
     9541 cccggtatag ttaagactct tttcgcctgc cttaagctcc ccgtcacagc tcatcacaat
     9601 caatgatgcg aaaaacttca tgtgttctcc gcccgggccc ttatccggat catcgggatc
     9661 aataatctgc ccctccatgt cgatcctgtt ctccgataca gtgattacgg catcctgcgg
     9721 cctggtcagc atgatatcca tgtcaagtcc gcccacttcc tgtgaacgca tgctgatcac
     9781 cataagatta tcgccggcag gcacataaac ttcatattca atcttctttc cgttcctgtc
     9841 gaagctgact ccggcaattc ctgttttcag gtcaagctca cgccggtatc cggttacccc
     9901 ggtggtgtca gcaaaccgga agaagatatc tcccacggtc tggtatgacc atgttgtgcc
     9961 cggtatgccg gccaggtact tttcagaaag ttcgtatgcc tctggtatct ttccgtcaag
    10021 tatcagctcc cttatcccgg ggaggttagc cagtgcacca ggattattgt ttggaacatt
    10081 caccccgccc cagagcgatt cctcattcac ctgcagacgc ccgaatgaag ggtcaccgaa
    10141 cagcatccct ccgagtcgcc cgttgcccac gggcagcgcc tcgatccact ctgcggcagg
    10201 cctgtcatac cacagaatat aatcatttga cgatctgttg tcgtttttgc atcctgaaaa
    10261 caggatctgt accaaaagga taataaccaa gctctttttc attgaatcac tatctattca
    10321 aagttttata caaaatgcat tgcaaagtct ccttgagggc tgcaaaccac agtctttata
    10381 ttaagacctg tccggggttt ggttaataag tcattcagca tttgcaagtg tttattcatc
    10441 ccggtgttta ttcatccagg tatttcttca tcccggaatt tattcctccc ggcgccttta
    10501 aagaatgtcc ggagtcttga ctctccggtt tttccagtct gattccctcc agcggcacga
    10561 aactgctgtc agccagctct gctatgcgga tattgttgca gatacccctg aacattatcc
    10621 tgatagtatt ctccacgttc tccctgatgg gttccatgat ccatgaaaac tcttccggcc
    10681 cgttctcaat cgactgctct gtctccagac ggatttcctc cttcagcctg tcagccttct
    10741 cagccaggtc atcgctggtc atccacctct tcaccgtcag gggcaactgc cttttgtact
    10801 ccagcgtctc gaagaactcc cattcaagct tgcgattgcc gaatgaaagg aaccgcgggt
    10861 ggatattggc cacaataagc tcgtcacttt caggtacata cttgaatcta agctccttga
    10921 ggtcgatgcc gtacttggcc ttgagcgaga cccccagact gccgatatgc ctgatctcac
    10981 gatcatgcag catctctctc ctgttgaccg tccgcttgaa cctggtatct atctcgaaga
    11041 ggttcatctc aaggatgctc tttatctgtg ttatctggaa tttccgatcc ttcaggaacg
    11101 cattttcctc tgtcagcttc tcgtttatct tcgccagatc ctggttcttc tcccggaggc
    11161 tggagatgct gtcctcgaac tttgagacct tcacattcag ctggataagc tgttgtatgc
    11221 ccaggactat cagtccggcg gcaatgaata cagcgcccca gactatcagc ttgtttgcaa
    11281 gcagtatcac ggccacaagc attattactg caaccagcag gtaacgcttt atcccggcca
    11341 ttgtaaatct tgatctcttc atgtcgttgg gttcttgctg ttatgtgccc tgtggaagcc
    11401 ggtgaaaaaa gccctggcat tgatgccgag cgactggttc cggtatcccc cctcctgaac
    11461 tacaagtgtg ggcagtttca ggcttccaat catctccccg ttagtcgtaa agttactgtg
    11521 agtaaagctc catgtcccgg tcggatctcc cttcgccggg tcgaggccaa gtgcaactat
    11581 cagataatca ggattgtatt tcttgatgat attaagtgca cgactcagcg cttttacata
    11641 ctgctcatgc gtcgccttct ccggaagagg aaagttatga ttgaaaccga taccatcacc
    11701 ctcgcccttc tcctcttcaa atccggagaa atagggatat gcaaatgagg ggtggccgtg
    11761 tatggagatg gttagcacgt cactccggct gtagaagatc tgctgctgcc cgtttccgtg
    11821 atgataatca atatccagta ttgctaccct gctgctgcgg ctcaggtagt gtgcagcaat
    11881 cgcacagttg ttgaaatagc agaacccgcc gaagactgat ctctcggtat ggtgcccagg
    11941 cggcctggta aggacgtagg cgtatcgcgt tcctgccagc atctcatcag cagcactcaa
    12001 gacgcagttg actccccatc gtgctgcaag gtatgcctcc tggttgagcg gagtaaatgt
    12061 gtcaaagcaa taataccctg ccctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnngattgc agcaggtccg gcagcacgcg gattatggtt gatctgccgg cggtgctccc
    12241 ccggtatcgc ttacagtaaa tttgccggcg ccgctccccc ggtactcata ccaggtcgca
    12301 cgcttcgggc ggcatccact tggcatcacg cccttcccac ttgatgttgc aacctatggg
    12361 attggtaaca ggtacgctga tctgacggtt cgaagtgagc tcctccagaa ccctgtcaag
    12421 gtcattgacc gttatacgtg aggcatctct tggcgagtca acagccctgc cggtataaac
    12481 cagcacccgg tcacggttga aaacaaagaa gtgcggcgtt ttgagcgcac cgtaatcttt
    12541 tactaccgtc tgctccctgt catgcaggta tttccacggg aaattgtgct ccttcatcct
    12601 tttaatcatg ttcgggaagc tgtcctcctc ataggtgtgc tcactgttgg agttgatgcc
    12661 aataaaagca accccttttt cacggaattt ctccgctgtt gccctcgtca cctcgtcgga
    12721 accggtcaca tacggacagt ggttgcatgt gaagaaaatc accaggaaag gatgatcatt
    12781 aaagtctgac aggctgtatt cccttccgtc agtggcagga agcctgaaat ctatagcctt
    12841 ctggccgggt tgaagtgtat aactcatatc actgcttgat ttacggttga ttattcattc
    12901 ttttttgcta agataatgac cgcaaccggt ttttcaagct catgaccctc gccggtgatg
    12961 atatgcatta cctgttacct gtcgagccaa tcgttctctt taaggatcga gagtcctttg
    13021 attattatac ggttcagact ttccggcggc ctgttgaaca gaccaacata cagcagatcc
    13081 ttctccggga ccgagaaatg aaacgccttg aatcctccct gatctccggt gtgatacaca
    13141 aacttcaccc cgaatttgtt ggcctgcccc agtatctgct cctcaccgat gaaccagctg
    13201 taaccgatga agggtggctc atctcctgcc catgcatcag gccggcagat tgtccttgac
    13261 ttgttggtga gttccgcacc gaggaagaga ttctctctga tggccttctc atacaatgca
    13321 agcccggtca ccgaactcca gacacctccg ttaccagccg ccgcgaatgt gggaaactca
    13381 ccatagtcag actccgtcca tacgccgttc accatctcat atgcatgcga tacacctgtc
    13441 tcagggtgag gtccgtcagt gatggtgctc gtagccatcc cggcaggctt gaaaatcctt
    13501 tcagcaatga aatcctgcca cttctgaccg ctgaccttct caatgatcag tgcaagggca
    13561 ttataagcgg ggttggaata ttcatacctg ctgccaggct caaaaacaag atcctctgcc
    13621 tgcgtcaccg gagcccagtt ctcgtaatct ttcgcggtga ggaaaaactc ggggttctca
    13681 ctcaccggcc tgttgtcagg gagtcctgat gtatgggtca gcaggtgcat tatcgtcacc
    13741 ctgtctgcta tttccgtgct tttaaactcg gggaagtatt tgctcagggg atcatcaacc
    13801 gagagcttcc cttcttcctg gagaatcagt atcccattgg ctacgaaggt cttggagata
    13861 gaacccagat taaatatggt gttttcggtg atcttttccc cggtcactat gtcggctatg
    13921 ccgaagcttt ttgaatatgc gatgctgtct cctttcatga caaggagcgc accgccgggt
    13981 tcaccggctg caaattcgga tgcaagcaac gaatcaagcg atgtaaacat ggtctttccg
    14041 gtttgttttg ttgaacatga aactgccagc aggcagaccg acaggagcaa ggctgttctc
    14101 atttaatttg ttttttggat aatgttatgc ttaacgactc tatccccggg gttaaccgcc
    14161 ccccccggtg caattctgag gagatagttc acttttccgt cagccggttg tatttacgca
    14221 ttatctccag cgtcttctca acgggcagcg cctcaatgat catgccgttc ctggccctgg
    14281 tggactcagc agcaaagagc gagttgatga ttgcctcctc ggttgcttcc gcagcagcct
    14341 gaaacaacag gtccatctca tcatttctga tctcgcgcca ctgctcgtac atcgaatcgg
    14401 aagagtaggg gatacgtagc tcctcagccg tggatactgc gattatgtaa tcaccgctgc
    14461 cgttcgagcc tgatgatcct gtccgcatca tgccggcaaa agccctggaa gccagccttt
    14521 tcagattcct ggaggtgagg ggcgcgtcgg tcatgactac aatcatacat gacccgtctt
    14581 ccttatattc attcacggtg tttttgaagc tgtaacgccc gagctcctga cctacaggaa
    14641 ccccgtttat ctgtagtatc cctccgtagt ttgtctgcac cagcacaccc accgtatagc
    14701 ctcccctgac ggcgggaagc acccgcgacg atgtgcctat ccctcccttg aatccgaaac
    14761 atactgttcc ggcgccggcg ccaacattac cttcggcaac aggaccgcct gccgcattcc
    14821 tgatcgcctc cagaacatgc tccatggtga catgccttcc ccggatgtca ttgatcctgg
    14881 catcactggt ctcgccgaca actgcgttga cactcatcac ccgctcattg ccgggctgcg
    14941 agagggtata ctcaatcact gcagcaacag cggtcgccac attaagcgtg ttcgtcagca
    15001 ctatcggcgt ctccaggttg cccagctctt ctacctgcgt gtatccggcc agcttgccga
    15061 acccgttgcc cacgaagata cctgccggga ccttctgctg aaaaatgttt ccctcgtggg
    15121 gaaggatggc tgtgactccc gttcttaccg agtcgccctc ccacagtgtc acctgaccca
    15181 cctttacgcc gggcacatcc gttatggcat tgttgcttcc tgtacgcagt ataccgatct
    15241 ctattccgta gtctctggct ctcttcatct gggcatatgt gttatgatgg ttcagtaccg
    15301 gcagcatcag caatattgca gccactgtac tgataatttg tctgttattc atcatttccg
    15361 tattcgctta ctgttcttca gtgcggtcaa acaccttttt cccgcctacc catgtctgct
    15421 gcaccttcat atcatatatc tcaacagggt cgatggcggt gatgtcacgg tcaaggacca
    15481 cgaagtcggc cagcttgccc ggctccagtg tgcctttgaa atcctcctcg aacgaggcat
    15541 aggcagcatt gatggtatat gccttcagcg cctcctcaac ggtgatcttc tgttcaggta
    15601 tccagccgcc ggggttcata ccgtcaagtg tccggcgtgt tgcggcagca tatatccctt
    15661 caagaggcgt tgcaggcgca acgaaccagt cgctcccgaa agcaagggtt gctccgctgt
    15721 caagcagcga gcggaacgca tatgttgttt tggcccgctc cggcccgatg tactcttcgg
    15781 cccacctgcc gtcatcaatg gcatgatatg gctgcatgct tgcaatcacc ttcagtttcc
    15841 cgtaacgctc aatatcagca ggagcaatgt gctgtgagtg ctcaatcctg aaccgcctgt
    15901 cacggtcacc gttctcatcg gcaacacgct cgtaaatatt caaaagggtg tttattgcgc
    15961 ggtcgccgat cgcgtggatt gccacctgca aacctgcctt gtcagcagct gaaacccacg
    16021 tgtaaaggtc ttgctcactg ttaacgaaaa gtccggtgtc agtcggtaga tcggtgtacg
    16081 gttcgaggaa ggctgccgta tgtgatccga gtgagccgtc aacaaacccc ttcacaccac
    16141 cggtgcttaa ccacctgtcg ccacgcccgg tacgctcaac ctgttccttc atatccgccc
    16201 agcgacttat tgtctgcagg tagtagatgc gtgttatctg tctgccgctt gcgcgaacgc
    16261 ggtcaaggcc gtcagcatag gctgctgcgt caagggcatg caccgaagtg accccgttgg
    16321 aggccaggta cttcatggcg gcaaccagtg ccaggtctgc ctcttcggcc gactgctccg
    16381 ggattttgac ggagatcaga tccatggcat tgtccttgaa aatgccggtc agctcgccgc
    16441 gtgcgtctct ctctatagtc cctccggtga catcctttac cttgcgatca atgccggcaa
    16501 tcttcattgc cagagagttg gccagtgcca tgtgaccgtc aaggcgcgag acaaaaaccg
    16561 gatgatcaga ggtaacggag tcaatccatg accttgcggg aaggctctcc caacccatcc
    16621 cgtcccagtc gcctccgagt atccaggtcc cgggcttctg tgtggcggca aactccctga
    16681 ttcgttttat gaactcctcc ggagtggagg catcacggag ctgcacagag gcaagattgg
    16741 accctccctg caacaggtga acgtgcgtgt caatgaatcc cgggacaaca aacatgccgc
    16801 cgaggtcaat gagtgtgccg gatacggatc tgtgcttcat cacttcgcgg ttgctgccaa
    16861 tggcaacaat ggtatcaccg gagaccgcca tggcctccgc aaacggctga tccgcgttcc
    16921 ccgtccagat cctcgcattg gttataatca tatcagatgt ttgagttgaa ttgttgcatg
    16981 agatggcaac cagtaacaag atgatggctg cagggaaaag tgctcttttc atacatggca
    17041 tgatttagga gtcggtttcg ggataatgct tttccttaaa ctcattgaga agcgggatgg
    17101 ccttctcaag gttttcttca aggatataga gatccatgac cggcggcacg gctcccaggt
    17161 atccgagccc cgagtcattc ttaatcagac tctttatgcc ggtctcttca agcatctcca
    17221 tcagcaggag ggtagtgggt tccgggccgg tgaataccct tatcagtttt tcttcgtttt
    17281 ccataatgtg cttttttgta ggtttatcag ggtaaagggt gcctcccctg gtcagacacg
    17341 agacaccgag cgaaagaagg atacctgtcc agcccaccgc ctttcgcaga gaaagatatg
    17401 aatagagctg tttcgtatgg tctgttttaa gttccataaa atcagtaaac tcatgccagg
    17461 ccggctgcac ccggggatgc ctgcgacagc tggtctgctc tgatcggtta aagtacaata
    17521 attattcata tccggttcag ggtgaaacaa aaaatcccgt agcgccggct acgggatttc
    17581 tgtgaattcc agcatggttt attgccggat tcagattaac attttttgct taacaagtat
    17641 tgaatgaatg atttactctc tgaacagggt ggatcacagc agcaaatatg atagccactg
    17701 ctgaggtgta catgatatcc ctgtcggtct ccggggcata gatgccggaa ttatcaaggt
    17761 tcctgatggt aatcctgttg aaactgcaat caaaatcccg gacaggatga atacagctat
    17821 tcaggacttt aatgatgaat gctactgtta ttagcatcgt gaccctcagg gttctgccga
    17881 tgctttttct catcggtccg gcgtatctgt ttaaagtaag tctcatatca cttttcattc
    17941 aggaattcag ttcattgaag agcgcgatcc ccttttcgtc aaaggtgcag ttaagccatt
    18001 cgttgttgat ctccgcacca cttttccggt agaaactgat agccggctca ttccagtcga
    18061 gcacctgcca tcgcacacgc ttgcagttct cttcccaggc ctcccgcatc acccgcttca
    18121 tcagagcttc accgattccc cgtttccgga attgcttgct gataatgata tcctcgaggt
    18181 agagcgattt tcccacccag gtgaaatagg cgaagaaata cagggccatc cccactatgc
    18241 cgtgatctgc actctccgcc acgaaacagc ggaagtaatc cctctcccgg cccatctgct
    18301 ccaccgtgtt ggtaacggct tcaggagccc gctcatactc tgccagttca tttatcaggg
    18361 ccaggattgc aggaaaatcc tcttcgtttg ctcttctgat atttactttc atacgcttgt
    18421 ctgctttttc aggtaatgaa tcctgtcagg ctttactgcc ttttatgaga gctttcatct
    18481 cattgaagtg atataccctg aagccaggtt cttcgatact cgcggcagca atcatggcct
    18541 gtgccacgtt aaggcctttt atgggtttgt acttcgccgg gaagagaaaa gagagcagag
    18601 gagtaaaaat cttcgagatc tcttccgcca ggcggaactc attccgcttg cccataagca
    18661 gtgagggccg gaaaacggaa acggagggga tgcccatcag cgcaacggcc tcctcaacct
    18721 cacccttgaa cctcagatag aagttgctgc tctttgc
//
LOCUS       DAOPBK010000027        18608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3076_length_18608_cov_5.447852, whole genome shotgun sequence.
ACCESSION   DAOPBK010000027 DAOPBK010000000
VERSION     DAOPBK010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18608
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3076_length_18608_cov_5.447852"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLA17_02765"
     CDS             complement(<1..712)
                     /locus_tag="PLA17_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPK84364.1"
                     /translation="MRLRIPAISLVILICSSAAGQIEFSSGSGYRYLKGINASDLPAD
                     WMTSQYNDSEWSEGNAPFRYGDGTGGVVLTDMMNSYSTLYLRSSFNAFSIDRIKTISF
                     RVNYDDGFVIWVNGTRVMARNAPATLSYNALATANHESGTFETFQLDSSDVQLVEGTN
                     LLCIQAFNVSLNSSDFYFDMAVNASLSLPEVPETLPLNFDHEAGFCETPFDLVISSGQ
                     PGYDVIYTIDGSNPQTSST"
     gene            complement(981..1202)
                     /locus_tag="PLA17_02770"
     CDS             complement(981..1202)
                     /locus_tag="PLA17_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84365.1"
                     /translation="MDYNFHIMSLPHGRTYMMNLTKNIVVRSSPGKLRKFYAKEKGGK
                     EFELNHLSALLQDTLKAARKITRVEYAQF"
     gene            1414..2754
                     /locus_tag="PLA17_02775"
     CDS             1414..2754
                     /locus_tag="PLA17_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK84366.1"
                     /translation="MNRGSGRISCIIAVAALMVLITPCVRGQTVNEPGAMPGSADDSL
                     GCVDYISWYKAAMNEGNISEALHPWRKALAGCPDISEELYTDGELLYRSLFDRTGEQE
                     YIDTIMLILTQRTYFYNNKPSNDLHKTEILLELAGDDPAYLGLCYNILAEAAGSFPEQ
                     MECRHYVQMAALAASLYVMEVIDGEELGNAFVTAIGTVDRRMENHIAGCNIAEDLNNM
                     ETFYRTSGAMTCEGLETLYGGKLDRNFRDTAFVSKLFSMTQEAGCTRSDLYYNVAVKM
                     FANDRSAKNAVRLAELNADAGNNDKAISYFTEAYNRDTNSVVRSDVLIRVAMMELGQG
                     KRQEARDRAEHAWQLNKKNAAALMLLAECYAGAELGNTFDNHAAYWVAADYLDAAVKA
                     DPSLRKEAEIKIKEYMQNFPTREECFYRRILDEGSIFTVGGWVNEVTRVRFRRE"
     gene            2891..3544
                     /locus_tag="PLA17_02780"
     CDS             2891..3544
                     /locus_tag="PLA17_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84367.1"
                     /translation="MKRIIAVLIFSLVMFPSFSQRQGVELSHYVFPDFTDGSVLMKSG
                     NRLQALLNYNALSEEMIFVDKDRKLAISREEKEKVDTVYIKGRKFFVLDSRFLELLYQ
                     SGYELYAEHKCDVKFPGQPAAYGGTSETSSVTAISGIYSGGVLYELKLPEDFKIKPYL
                     VYWLKKDGKINKFVNLKQLNKIFRDRKDQIQEYVSSNKTDYVDMESMVRLIRYLDAD"
     gene            3534..4157
                     /locus_tag="PLA17_02785"
     CDS             3534..4157
                     /locus_tag="PLA17_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84368.1"
                     /translation="MLTDGFSTCCGSPEYNAAYIDDPDSTNGATLPVYHTYLLTADLE
                     TGEIVLDKEFDLGEGVFLCTHFFDPVNKLYLLQRDDNKLLLVNPVTGTITKTVDIGKP
                     LINVVFNSDDGHLLSLIPASEPGICLIEVYEPSTGELISSRQVNGIDSYHFCMSGYDP
                     DSKCYLCVTDDNRVLFIEPETGTIARTIKLDYQLDDIRFLRKQAESF"
     gene            complement(4419..4492)
                     /locus_tag="PLA17_02790"
     tRNA            complement(4419..4492)
                     /locus_tag="PLA17_02790"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4456..4458),aa:Ala,seq:ggc)
     gene            4642..5328
                     /locus_tag="PLA17_02795"
     CDS             4642..5328
                     /locus_tag="PLA17_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPK84369.1"
                     /translation="MELKKSEKANLENKKSLFIQIGLIIALIVCIGAMEWTSGQKKES
                     VFDGMTEEVIEEEQVPVTEETPPEEMPPPEVTVTDIFEIVQDDVVIDREVRFEDDETS
                     IDKVVEIYAPVLQAEAEEVEEEVFVIVEDMPKFRGGDINKFREWVQKRVRYPELASEN
                     GIQGRVYITFVVEPSGNVSNVTVSRSVDQLLDDAAKEAVMASPKWEPGMQRGRPVRVR
                     YSIPIIFQLE"
     gene            5401..6573
                     /locus_tag="PLA17_02800"
     CDS             5401..6573
                     /locus_tag="PLA17_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84370.1"
                     /translation="MKRTIGILFIAAMLMSGCGSSKKQLEIGNYDAAISKAVAELRKK
                     PDSEKDIETLERAMNIALEQDNERIRFLKMEGRANAWDEIYMIYQKMNDRQSAVRTVT
                     PIRYQGRSIEWPYVDYMQEMVDAKKKAADFYYEHGQELMKNNTKESYRQAYAEFARAR
                     DYMGDYPNIDQMMQESHYLGISRAWVTVKNYSAIQFPEEFEKELLALDLPRLNSEWVE
                     YHTTMPGNDTQIDYQININLRMIAVTPDRQYQRDTLVKATVEDGFEYVKDARGNVMKD
                     SLGNDIKVKKFKELQCALIQTVQAKECVIQGDVEMLSFYPERTIKKEPIGARSNFEHI
                     SARAIGDVNALSEEQKKMLQSQPVQFPMDIDMVIMCTENLKSAIRGFMQANRRYIN"
     gene            6755..7927
                     /gene="hflX"
                     /locus_tag="PLA17_02805"
     CDS             6755..7927
                     /gene="hflX"
                     /locus_tag="PLA17_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPK84371.1"
                     /translation="MIGVITPEISEARARDYLDELAFLAETAGAVPVRKFLQRVEHPN
                     PRTFVNKGKIEEIAAFIAAGEITLAIFDDELTPSQMRNIENDLNCRIIDRTNLILDIF
                     AGRARTSHARTQVELAQYQYLLPRLTGMWTHLERQRGGIGLRGPGETEIETDRRIIRD
                     KITKLKEQLKKIDTQMSTRRKGREKMVRVALVGYTNVGKSTLMNLISKSDLFAENKLF
                     ATLDTTVRKVVLGNLPFLLSDTVGFIRKLPHDLVESFKSTLDEVREADMLLHVIDISH
                     PGFEEQVTVVNTTLQELESLNKPTIYVFNKTDLFTFTRKDEDDLTPVKRENLSLEELK
                     KTWMAGAGDQLTVFISARNRDNLDELRRILYTEVKKIHVQRYPYNDFLFESEEPVI"
     gene            complement(7920..8912)
                     /locus_tag="PLA17_02810"
     CDS             complement(7920..8912)
                     /locus_tag="PLA17_02810"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPK84372.1"
                     /translation="MKVAVVGVTGMVGRMMLKVLEERQFPVDRLFPAASERSVGKEVM
                     FRGKPVKVVSVMEAVESAPDVVLFSAGASVSKEWAPVFAENGATVIDNSSAWRMEKGI
                     PLVIPEINAAAITSATRIIANPNCSTIQLLMVIAPLHKEYRIRRLVISTYQSVTGTGI
                     RARTQLENERRGLGGEMAYPYPIDLNCIPQCDSFLDNGYTKEEMKLENETRKILDDED
                     IRITATAVRVPVTGGHSESVNIEFAREFDLDRVRALLASTSGVVLYDDPSQNKYPMPL
                     TVHDRDEVFVGRIRRDFSQEKSLNMWIVADNLRKGAATNAVQTAEYMMSAGLIK"
     gene            8993..10693
                     /locus_tag="PLA17_02815"
     CDS             8993..10693
                     /locus_tag="PLA17_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPK84373.1"
                     /translation="MKKIILLPLLPLYLSVTAQTGNDAFPFPATGDIVITEIMADPTP
                     SRGLPEREYLEIFNRSSDTLGLKGIMLIADKDTAYLSDGLIAPGEWIILCSTGSRTYL
                     LPFGRVMAVKSFPTLNDAGELISLRTPDGSLIHSVSYSPEFLGDGPRSGGGWSAELTD
                     TDNPFHEPDAWSPSTDPSGGTPGRVNSTVTSVADTRCPSVIAAWPLSEEVVAVLFDET
                     VIPAESGPWLADGVETMPALSGDPADRTLLVTLAGKMDPGIVLTLQFPPGLTDFAGNQ
                     PCTGDIRTGLPSDPAPGEIMFNELMPEPSPGCSEYLELFNNSDKIFDLSALLLAGSSR
                     GAATAFTALHRQLLPGAFVALTTGREDFASIYPCGDDKSVFRADRLPALPDGGGTLLL
                     YDRSLNVIDRVSYTPDMHMIFLSGTKGVALEKVSPGLPSDVASSWHSASGVCGFGTPG
                     APNSVLAGDTGETGGLSLSSGRISPDGDGFEDVISVGVFPGGEGNVITVTIFNDRGYP
                     VRRLAERVTADAGARFVWDGVSDSGARLPAGLYMIVAESFNTAGLSRRWKKVCALLYR
                     "
     gene            complement(10721..11530)
                     /locus_tag="PLA17_02820"
     CDS             complement(10721..11530)
                     /locus_tag="PLA17_02820"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPK84374.1"
                     /translation="MLEKIIKTSEFLVSKGMKDPDAGIVLGTGLGGLTAEIDIQVEIP
                     YEDIPDFPVSTVKGHAGKLIYGDFGGKKVVAMKGRFHYYEGYGSEQVTFPIRVMKELG
                     IRALFLSNAAGGVNPDFRIGDIMIIDDHICLVDNPLIGPNDDRIGPRFPDMSQPYDKK
                     LIEKAITIASELNIPVSRGVYLSTTGPTFETPAEFRFFRIIGADAVGMSTVPEVITAR
                     HMNLPVFAVSIITDLGVEGKIEYTTHESVQAAAEHSEEKMTKIMKELIAAL"
     gene            complement(11502..12566)
                     /gene="lpxK"
                     /locus_tag="PLA17_02825"
     CDS             complement(11502..12566)
                     /gene="lpxK"
                     /locus_tag="PLA17_02825"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPK84375.1"
                     /translation="MIKPGRLFLWAASKIYGMAVALRNRLYDLGIFRSHSFSLPVICV
                     GNITAGGTGKTTHVIYLAEKLSSIASVAILSRGYLRKSRGFLKVTADSTTALSGDEPL
                     LMAQNLPQAQVFVDRDRVNGIREIMRKEPGIRVVILDDGFQHRAVRAGMNIILTDWTR
                     LMTRDRLLPLGMLRENIRAVNRADAVIVTKTPGVASPSAREEIRRELMAAGACETIFF
                     TSLAYGQPLHLCSNSTGEIRQTTSVLLVTGIANPAPLKEYLESIAGQVTGIAFPDHHA
                     YTQADIGRISSAYDSLSGPDKLIVTTAKDGVRLKEITNIADHVRQALYYLPVRVHFIE
                     DEKLFLNKVYSYAGKDYQNF"
     gene            complement(12568..14334)
                     /gene="sppA"
                     /locus_tag="PLA17_02830"
     CDS             complement(12568..14334)
                     /gene="sppA"
                     /locus_tag="PLA17_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPK84376.1"
                     /translation="MKTFLKMLLAVIAGLIITSLLFFVVMLSALSGMAAAGKKPATIP
                     EKTVLVMKTGALIPDRTQNNPFTMIDPMTRSFTQTPGLNEILKNLKKAAEDEKVSGLL
                     IENGTMLSGWATADELRTALLEFKKSGKFVYSYSDYMLMQESYFISTAADAIWINPTS
                     MFDFKGLAAEVTFYKDALEKLGVEVQVIRHGKFKGAVEPFILDRLSDENRLQITRYVG
                     SIWEHVVKSISDSRGIPAERIMNLADSLAGYDPALMTTAGLIDGTIYRDQLEDSIRSA
                     AGIDSGKKINYVSMSKYSNVTVKHSAATGKDKIAVLFAEGSIVMGDGNDTNIGGNRYA
                     EELRKLRLDSTVKAIVFRVNSPGGNAIASDQIWREVELTDKVKPVVVSMGNYAASGGY
                     YISAAARRIIASPVTITGSIGVFGLIPNAKTLLDKKLGISYDVVRTNAYSDSPSLTRP
                     LSAFERNAIQGSVERTYSTFTGVVAEGREMSQTAVDSIGQGHVWSGADAIPIGLVDSF
                     GGLRDAISEAASLAGLEEYRTIEKPETSDFYTRLKQLSGDIRMKAIRRELGEVSRYYL
                     DLKEIISSGGIQAAMPYYLEIR"
     gene            14407..14919
                     /gene="folK"
                     /locus_tag="PLA17_02835"
     CDS             14407..14919
                     /gene="folK"
                     /locus_tag="PLA17_02835"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPK84377.1"
                     /translation="MKLSLCSEVIAGLGSNLGERHAALQRALALLREEAGEITALSSV
                     WETEPWGFDADEQFLNMVAVIRTFHSPVQLIQLFRSIEGRMGRRRSGGGRYESRVIDL
                     DILLWEERVISIPGLEVPHPKIADRRFVLKPLSEVAPAAVHPVTGLTVAEMLQLCADN
                     SDVRLAARQL"
     gene            complement(14977..15654)
                     /gene="radC"
                     /locus_tag="PLA17_02840"
     CDS             complement(14977..15654)
                     /gene="radC"
                     /locus_tag="PLA17_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPK84378.1"
                     /translation="MKITDWAVEDRPRERLWNKGPASLSDAELLAILIRSGTRNNSAL
                     DLARELLAMAGNSLSQLGRLSAGEIKKIKGMGEAKAVTIAAALELGRRRKLAEAADNP
                     QIRSSADVFSIFSPLMEDLSHEEFWILFLNRANRVTGRMKISQGGVSGTVTDVRIVMK
                     KAIETLASGLIICHNHPSGNTSPSDSDIRITLKIKEAGALMDIQLLDHIIVAGKDYYS
                     FADSGAL"
     gene            complement(15746..16000)
                     /gene="rpsT"
                     /locus_tag="PLA17_02845"
     CDS             complement(15746..16000)
                     /gene="rpsT"
                     /locus_tag="PLA17_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPK84379.1"
                     /translation="MANHKSALKTVRKTETRKADNKYYARTMRNALKRMRNTTGKAEA
                     EKMLPELNAMLDKLAKKNVIHKNKASNLKSSITKHAQSLK"
     gene            complement(16020..16094)
                     /locus_tag="PLA17_02850"
     tRNA            complement(16020..16094)
                     /locus_tag="PLA17_02850"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16059..16061),aa:Glu,seq:ttc)
     gene            complement(16212..16682)
                     /locus_tag="PLA17_02855"
     CDS             complement(16212..16682)
                     /locus_tag="PLA17_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84380.1"
                     /translation="MMKRTLTVILVLAALTFGVQAQSYKSAIGLRLGDPSGITFKTFI
                     NSTNALELIAGTGYWGHNFAVTGFYQWQNPTNWAPNLDWFVGPGAHVGFWNDYWKDEY
                     NTGILIGFDGIIGLEYTFDDIPLNLGFGVGPSLQVTSGPGFFYWNGGFSVRYIF"
     gene            complement(16749..17558)
                     /gene="zupT"
                     /locus_tag="PLA17_02860"
     CDS             complement(16749..17558)
                     /gene="zupT"
                     /locus_tag="PLA17_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPK84381.1"
                     /translation="MNPDFNTVLFAFLLTLFAGLSTGVGSTIAFFAKKTNVKFLTISL
                     GFSAGVMIYVAFVEIFVKGREVLTEAMGIRQGWWAAVIAFFAGIALIGIIDRLIPSQE
                     NPHEFKKIEGGEEERQSGRLLRMGAMTAIAIGIHNFPEGLATFTAALSDPQIGIAIAV
                     AVAIHNIPEGIAISVPVYYATGSKRKAFRLSFLSGLAEPVGALAGYLLLLPVMSDVLM
                     GILFSFVAGIMVFISLDELLPAAEKYGEHHLSIYGLVSGMAVMALSLLLFK"
     gene            17635..18474
                     /locus_tag="PLA17_02865"
     CDS             17635..18474
                     /locus_tag="PLA17_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK84382.1"
                     /translation="MKLSLFNISNFRVDGGAMFGVIPKALWSRQTESDELNTIVMSLT
                     SPVIETEGRVILVDAGWGDKQDEKFFRYTYLHGGSGLAGGLAECGYTPDDITDVIITH
                     LHADHCGGCFINSPGGGTVPFFPNATWHISRAQWEWANESNLREEDAFLPENIRPFGE
                     SGKLNFIEEPGELYRGVSLRICYGHTPGMLIPIISCYGRTMVYTGDLIPTVAHIPLLW
                     NMAYDLLPLVTIAEKQEILQEALQNDWLLLFQHDPAHECCSLSETPKGIRAGTRGRLS
                     DFI"
BASE COUNT         4607 a         4571 c         4769 g         4661 t
ORIGIN      
        1 cagttgatga ggtctgcggg ttgctgccat caatggtgta gatgacatca taaccaggct
       61 gacctgatga gatgaccaga tcaaacggag tttcacagaa tcctgcctca tggtcaaaat
      121 tcagcggcag tgtctccggc acctcaggca gggagagtga cgcattaaca gccatgtcaa
      181 aatagaagtc ggaactgttc agtgacacgt tgaaggcctg aatgcacagc agatttgtcc
      241 cttcaaccag ctgtacgtcg ctcgaatcaa gctggaaggt ctcaaatgta cccgactcat
      301 ggtttgctgt tgccagggca ttgtaagaga gtgtggccgg agcattgcgg gccataaccc
      361 tggtaccgtt gacccatatt acgaaaccgt catcataatt gaccctgaaa cttatggtct
      421 tgatcctgtc tatggaaaag gcattgaatg aacttcgcag gtaaagggta gagtagctgt
      481 tcatcatgtc agtgagtact actccgcccg ttccgtctcc atagcggaaa ggggcgttcc
      541 cctcgctcca ttcagagtca ttgtattggg aagtcatcca gtctgccgga aggtctgaag
      601 cgttgatacc tttaaggtac ctgtatccgg aaccggaact gaattctatc tgtcccgcag
      661 cagaggaaca gataaggatg acaagactta ttgcgggaat cctcaggcgc atctctttac
      721 aacaatcccg ggcatcaggt cattctgcat caacggcccg atttcccggg aaaaacaaag
      781 gtagctcatt tttctttata cgccccgcgc caacggatgt taccatatgt tgtacagaat
      841 gtaaaagccg gaatttaaaa aagctgcaga gggctgcatg gaacaatgcc ccttcaggtg
      901 ctgcacggaa caacgccctt caggtgccgc atatccttaa tgatccgtct ctcagcacct
      961 gccgtgctga cagggcgaaa tcagaactga gcatactcca cccttgtgat cttcctggct
     1021 gccttgaggg tatcctgcaa aagggcgctc aggtggttaa gctcaaattc cttcccgcct
     1081 ttttcctttg cataaaactt tctgagtttc cctggtgaag accttacaac aatgttctta
     1141 gtgaggttca tcatgtaggt tctcccgtgt ggcagactca ttatgtgaaa attgtagtcc
     1201 atattgaatg aaatacctgt tgttggtcaa atcacgtcag cgttttacgg atgcctgctt
     1261 ccgaaatcgg ttacattttc ttcatccgta attatttaac gtcagatata taaaaagatt
     1321 gtttgccccc tctattttgg atacgaattg agacagccgg gagttatata tcatataatg
     1381 aaagtcacct tatcataaac tgattgaaat aagatgaaca ggggaagtgg ccggatctcg
     1441 tgcattattg cggtggcagc acttatggtg cttattacgc cgtgcgtcag gggccagacg
     1501 gtgaatgaac cgggcgcaat gccgggaagt gctgatgaca gccttggttg cgttgattac
     1561 atctcatggt acaaggctgc catgaatgag ggcaacatca gtgaagcgtt acacccctgg
     1621 aggaaggcgc tggccggatg ccctgacatt tctgaagaac tttatactga cggtgaactg
     1681 ctctacaggt cactctttga cagaacgggc gagcaggagt acattgacac cataatgctg
     1741 atcctgacac agagaaccta cttttacaac aacaagccgt caaatgatct tcacaagacg
     1801 gaaattctcc ttgaactggc gggtgatgac ccggcatacc ttggcctgtg ttacaacatc
     1861 cttgctgagg ctgcagggag tttccccgaa cagatggaat gccgtcatta tgtgcagatg
     1921 gctgccctgg cggcaagcct gtatgtcatg gaggtgattg acggtgaaga gctgggcaat
     1981 gcctttgtca cggccatagg aaccgttgac aggcggatgg agaaccatat cgccggctgc
     2041 aacattgcag aagacctgaa taatatggaa accttttatc gcaccagcgg tgcgatgacc
     2101 tgcgaagggc ttgagacgct ctatggcggg aagctcgacc gcaatttcag ggacacggct
     2161 tttgtcagca agctgttttc gatgactcag gaggcaggat gcacccggtc ggatctctat
     2221 tataatgttg cagtaaagat gtttgccaat gaccggtcag cgaaaaacgc cgtcaggctg
     2281 gctgagctga acgctgatgc cggcaataat gacaaggcca tttcctactt caccgaagca
     2341 tataaccgtg acacaaacag tgtggttcgt tcagatgtcc ttatccgtgt tgcaatgatg
     2401 gagcttggcc aggggaaaag acaggaggcc agggaccgtg cggagcatgc gtggcagctg
     2461 aacaaaaaaa acgctgcggc gctgatgctc ctcgcagagt gttatgccgg cgccgagctg
     2521 ggcaacacgt ttgacaatca tgctgcctac tgggtggcgg ctgactatct tgatgcagcc
     2581 gttaaggctg atccttcact gaggaaggag gctgaaataa agataaagga gtacatgcag
     2641 aatttcccca cgagggagga gtgtttttac cgcaggatac ttgacgaggg gagcatcttc
     2701 actgtgggag gctgggtcaa tgaggtgacc cgagtaaggt tcaggagaga gtaatgaggc
     2761 aaatggaggg agacaaaatg cctttttgaa aggcaccttg ctgttaaact gttatttctg
     2821 tatagagtat cgttattgtt tttgtatttt tacggtgaaa gaaaatattc actgtcaaaa
     2881 tactatttag atgaaaagaa ttattgcagt tctgatattc agcctggtca tgttcccatc
     2941 cttttcccag agacagggcg ttgaactctc tcattacgtt tttcctgatt ttacagatgg
     3001 ttctgttctg atgaaatccg gtaaccggct tcaggctctg cttaactaca atgccttgtc
     3061 cgaggagatg atttttgtgg ataaagacag aaagctggcc ataagcaggg aggaaaagga
     3121 gaaggttgac actgtttaca taaagggaag gaagtttttt gtgcttgaca gccgttttct
     3181 cgagctgtta taccaatcgg gatacgagct gtacgctgaa cacaagtgcg atgtgaaatt
     3241 tccaggccaa ccggcagcct acggaggtac ttctgaaacc tcatccgtaa cagcaatttc
     3301 cggtatatac agcgggggag ttctgtatga gctgaaattg cctgaagatt tcaagataaa
     3361 accatacctc gtctattggc ttaaaaagga tgggaagatt aataaatttg tcaatctcaa
     3421 gcagctgaat aaaatattca gagacaggaa ggaccagatt caggagtatg tcagcagcaa
     3481 taagacagac tacgttgata tggagagtat ggtccggctg atcaggtatc tggatgctga
     3541 ctgacgggtt ttcaacctgc tgcggcagcc ctgaatacaa cgctgcatac attgatgacc
     3601 ctgacagtac caatggcgcc acactgccgg tatatcatac ataccttctg acagcagacc
     3661 tggagacagg ggagatagtt cttgacaagg aatttgacct gggcgaaggt gtttttctct
     3721 gcactcattt ctttgaccct gtgaataaac tgtacctcct gcagagagat gataataagc
     3781 tgcttcttgt caaccctgtc acaggcacaa ttaccaaaac tgttgatatc ggcaaacccc
     3841 tgataaatgt tgttttcaac tctgatgacg ggcacctgct gtctctgata ccggcaagtg
     3901 aacctggaat atgcctgatt gaggtctacg agccctccac cggtgaactg ataagttcca
     3961 ggcaggtgaa cggcatcgac agctatcatt tctgtatgtc cggttatgat ccggactcga
     4021 aatgctatct gtgcgtaact gatgataata gagtgctttt cattgaacct gaaacaggaa
     4081 caatcgccag aacaatcaag ctcgactatc agcttgatga tatcagattc cttcgaaaac
     4141 aggctgagtc tttctgagag acaacccttt ttatcagtat ttatgaataa ttcaggctga
     4201 tcagaccgga tgaacgaatc aacaaactct gttatcagaa gagttcatta ggcaaaggag
     4261 atacaatccg ttataaaaac tgcaaaagga aagcatgtag ccttattcgg tataccggat
     4321 tatcaaatcg atatactttt ctgcccggga gacaaaaact aaaagccctg caaacgtcag
     4381 atttgcaggg tcttgtgccg agttgcattg tggttttgtg gagataaggg gaatcgaacc
     4441 cctggccttg tgactgccag tcacacgctc tagccaactg agctatatcc ccatactaat
     4501 aatgaactgc tccggtgcag gaaaagagtt cttttcgccc aaagcggtac aaatatagaa
     4561 attttcccta aatcagttaa tattcccctt tcggagatta caaacaattt tgtaactttg
     4621 aactgccata acagcattac aatggaactg aagaaatcag aaaaggcaaa cctcgagaac
     4681 aagaaatcgt tgttcatcca gataggcctt ataattgccc ttattgtctg tatcggtgcg
     4741 atggaatgga caagcggaca gaaaaaggaa tcggtctttg acggaatgac cgaggaggta
     4801 attgaggagg aacaagttcc cgtgactgaa gagactcccc ccgaagagat gcctccgcct
     4861 gaagtgactg tgactgacat tttcgaaatc gttcaggacg acgtggtcat tgacagagag
     4921 gtaagattcg aggatgatga aacatccata gacaaggtgg ttgagattta cgcccctgtc
     4981 cttcaggcag aggcggaaga ggtggaggaa gaagtattcg tcattgttga agatatgccc
     5041 aaattcaggg gtggtgatat taacaagttc cgcgagtggg tacagaaacg ggtccgttac
     5101 cctgaactgg catccgaaaa cggcatacag ggaagagtgt acatcacctt cgtggttgag
     5161 ccaagcggca atgtcagcaa cgttaccgtc tcccgcagcg tcgaccagct ccttgacgat
     5221 gccgcaaagg aagcagtgat ggcatcaccc aaatgggaac ctggcatgca acgaggccgg
     5281 ccggtgaggg tgagatattc aataccaatc atattccagc tagaataatc tgacgtttcc
     5341 agcgttatat aagaaccccg ccttcaaaat gaataacggg gtttttaaaa atctggaaat
     5401 atgaaacgga ccatcggtat cttatttatt gcagccatgc tcatgtcagg ctgcggatca
     5461 tcgaagaaac agcttgaaat cggcaactat gacgcggcca taagtaaagc tgtggctgaa
     5521 ctcagaaaaa agcccgacag tgaaaaagac atcgagaccc tcgaacgggc catgaacatc
     5581 gccctcgaac aggacaatga acggattcgt ttcctgaaga tggaaggcag agccaacgcc
     5641 tgggatgaga tctacatgat ttatcagaaa atgaacgacc gccagtcggc agtgcgcacg
     5701 gtgaccccca taaggtacca gggtagaagc atcgaatggc cttacgttga ttacatgcag
     5761 gagatggtcg atgccaagaa gaaggcggct gacttctact acgaacacgg ccaggagctg
     5821 atgaaaaaca acaccaagga atcataccgc caggcttacg ccgaatttgc acgcgccagg
     5881 gattacatgg gtgactatcc gaatattgac cagatgatgc aggaatcaca ttacctcggt
     5941 atcagccggg cctgggtcac ggttaaaaac tattccgcca tccagtttcc cgaagaattc
     6001 gaaaaggaac tgctggccct tgaccttcca cggctcaaca gcgaatgggt ggaataccac
     6061 accaccatgc ccggtaatga tacacagatc gattaccaga tcaacatcaa ccttcgcatg
     6121 atagccgtca ctcccgacag gcagtaccag cgtgacacac ttgtgaaagc gactgtagag
     6181 gacggtttcg aatatgtcaa ggacgccagg ggtaacgtga tgaaagactc gcttggcaat
     6241 gacatcaagg taaagaaatt caaagagctt cagtgtgctc ttatccagac cgtgcaggcc
     6301 aaggaatgtg tcatacaggg tgatgtggag atgctttcgt tctaccctga gaggacaata
     6361 aaaaaggagc ccatcggagc caggtcgaat ttcgaacata tttcggccag ggccatcggc
     6421 gatgtcaatg cactgtcaga ggagcagaaa aaaatgctgc agtctcaacc ggttcagttc
     6481 ccgatggata ttgacatggt aataatgtgt actgaaaatc taaagagtgc tatcaggggc
     6541 ttcatgcagg ccaaccgaag gtatattaac tagattgtat tatttttgac gccccggccc
     6601 tcacagaccg gggcggtttc tttccgtcac ttttgttatg gccttgtatt caccctcagg
     6661 ccaccgggtc aggggagcgc ttcccggccg gacaagcatg aagtgcagga ataatgatta
     6721 ttgataaacc ttctgaagaa tagaaagagc ggtattgata ggggtgatca ccccggagat
     6781 cagcgaggcc cgtgcccgcg attaccttga cgagcttgcg tttctggcag agacagccgg
     6841 cgcagtgccc gtcaggaaat tcctgcagcg cgttgaacat cccaatccga gaacctttgt
     6901 caacaagggg aagatagagg agatagctgc ctttatcgct gccggagaga tcacactggc
     6961 aatttttgac gatgagctca ctcccagcca gatgcgcaat atcgaaaatg atctcaactg
     7021 ccgtatcatt gaccgcacca acctcatact tgacattttt gcgggcaggg cccgcacctc
     7081 gcatgcccgt acccaggtgg agctggctca gtaccagtac cttcttccga gactcaccgg
     7141 catgtggact cacctcgaac ggcaacgggg aggtatcggg ttacgcggtc cgggcgagac
     7201 tgagattgag actgaccgcc gtatcattcg tgacaagatc acgaagctca aggagcagct
     7261 taagaagatc gacacccaga tgagtacccg tcgcaaaggg agggagaaaa tggtcagggt
     7321 tgcccttgtc ggctatacca acgtcgggaa gtcgaccctg atgaacctga tcagcaagag
     7381 tgacctgttt gcggaaaaca agctctttgc cacccttgac accaccgtga ggaaggtggt
     7441 gctgggcaac ctgcccttcc tgctttctga tacggtgggt ttcatccgca agctgccgca
     7501 tgatctggtt gagtcgttca agtcaaccct cgacgaggtc agggaggctg acatgctgct
     7561 tcatgtcata gatatttcac accccggttt cgaagaacag gtgacagtgg tgaataccac
     7621 cctccaggag ttggaatcac tcaacaaacc gacaatttat gtcttcaaca agacagacct
     7681 gttcaccttt accaggaagg atgaggatga tcttaccccg gtgaagcgcg agaatctctc
     7741 tcttgaagaa ctgaagaaga cctggatggc aggtgccggt gaccagctga ctgtattcat
     7801 ctcagccaga aaccgtgata atcttgatga gctgaggcgg atactgtaca cggaggtaaa
     7861 aaagattcac gtacagcgct atccctacaa tgatttcctg tttgaatctg aggagcctgt
     7921 catttaataa ggccggcaga catcatgtat tcagctgtct gcaccgcgtt ggttgcggcg
     7981 cccttgcgta agttatcagc tacaatccac atgttgaggc tcttctcctg tgagaaatcg
     8041 cgcctgatac gacccacaaa cacctcgtca cgatcatgaa ccgtgagcgg catggggtat
     8101 ttgttctgtg agggatcatc atacagcacc acaccgctgg ttgaggcaag cagcgctctc
     8161 accctgtcaa ggtcaaattc gcgggcaaac tcaatattga cagactctga atgccctccc
     8221 gtcaccggaa ctctcactgc cgtggccgtg atccgtatat cttcatcatc cagtatcttg
     8281 cgtgtctcgt tctccagctt catctcctcc tttgtgtaac cgttgtcaag gaagctgtcg
     8341 cactgcggaa tgcagttaag gtcgatgggg taggggtatg ccatttctcc gcccaatcca
     8401 cgtctctcgt tttcaagctg tgttctggct ctgataccgg tgccggtcac tgactggtag
     8461 gttgagatca caagcctcct tatgcggtat tccttgtgaa ggggtgcgat gaccatcagc
     8521 aactgtatgg ttgagcaatt gggattggca atgatacggg ttgcagaagt gattgcagca
     8581 gcatttatct cgggtatcac cagaggtatt cccttctcca ttctccaggc ggaggagttg
     8641 tcaattaccg tggcgccatt ttcggcgaag acaggagccc actcttttga gaccgatgcg
     8701 ccggctgaga acagtaccac atcaggagca gactcaacag cttccatcac gctcacaacc
     8761 tttaccggtt tgcccctgaa catcacctcc ttgcccactg aacgctctga tgctgcagga
     8821 aaaagcctgt caaccgggaa ttgtctctct tcaagaactt tcagcatcat tctgccaacc
     8881 attccggtta caccaacaac tgctactttc atttctttcg tgtttaaaaa aaatgttcta
     8941 actttataaa gataaaagtg cgacaaatct aatccttttt tagtcccgct gcatgaaaaa
     9001 gattatactg ttgccgctat tgccattata tctgtctgtt acggcacaga ccggcaatga
     9061 tgcctttccc ttcccggcca caggcgacat tgtcattacc gagataatgg cagatcccac
     9121 cccgtctcga ggcctgcctg aacgtgagta tctggagata ttcaaccgga gcagtgatac
     9181 cctcgggctt aaggggatca tgcttattgc agataaagat acggcgtatc tttctgacgg
     9241 tctgatagct cccggagagt ggatcatact ctgttccacc ggaagcagga cttaccttct
     9301 tccgtttggg agggttatgg ctgtgaaatc attccctact ctgaatgatg ccggggagct
     9361 gatatccctg cgtacccctg acggctcact gatacattct gtcagctaca gcccggagtt
     9421 tctgggtgac ggaccccgct ccggcggagg ctggtctgca gaacttaccg acactgacaa
     9481 tccgttccac gagcctgatg cctggagtcc ttcgacggat ccttcaggag gcacaccggg
     9541 aagggtaaat tcaaccgtca cgtctgttgc agacacgaga tgcccttcgg taatcgctgc
     9601 ctggcccctg tcagaagagg tggtggcagt gctttttgac gaaacggtta ttcccgcaga
     9661 atccgggcca tggctggccg acggcgttga gaccatgcct gcattgtcag gagaccccgc
     9721 tgaccgcacc cttctggtga ccctggcagg gaagatggac cctggtattg tactgaccct
     9781 gcagttcccc ccggggctga ccgactttgc cggtaatcag ccctgcacag gcgatatcag
     9841 aacaggtctt ccttctgatc ctgcaccggg cgagatcatg ttcaatgagc ttatgcctga
     9901 accttccccc ggatgcagcg aatacctgga actgtttaac aattcggata aaatatttga
     9961 cctctctgcc ctgttgcttg ccggaagcag caggggtgcg gcaacagctt ttaccgctct
    10021 gcatcgtcag cttcttcccg gagcctttgt ggcactcacg acaggaaggg aggatttcgc
    10081 ttcgatctac ccctgtggcg atgataagtc agtgttccgg gctgaccggc ttcctgcgct
    10141 gcctgatggc ggtggcacac tgctgctcta tgacagaagc ctcaacgtca tcgaccgggt
    10201 cagctacact cccgatatgc atatgatctt tctttcaggg accaaagggg tggcgctgga
    10261 gaaagtctca cccggcctgc catccgatgt tgcctccagc tggcattcag cttccggggt
    10321 atgtggtttc ggcacaccgg gagcaccaaa ttcggtttta gccggtgata ctggtgaaac
    10381 cggcggtctc tccctctcat caggcaggat ttcgcctgac ggcgacggat ttgaggatgt
    10441 gatctcagtg ggtgttttcc cgggagggga gggaaatgtc attacagtga cgatattcaa
    10501 tgaccggggt tatccggtga ggcggctggc agagagggtc actgctgatg caggagcacg
    10561 gtttgtctgg gacggggtgt cagacagtgg tgccaggctg ccggcaggtt tatacatgat
    10621 agttgccgaa tcattcaata ccgccggctt atcgcgccgg tggaaaaagg tatgtgccct
    10681 gctttacagg taacagtaaa attaatgact taattgccta tcagagggct gcgatgagct
    10741 ccttcatgat cttcgtcatc ttctcttcgc tgtgttctgc tgctgcctgg accgattcat
    10801 gggtggtgta ctcgatcttt ccttcaacgc ccaggtcggt gatgatactg accgcaaaaa
    10861 caggaagatt catgtgcctt gcagtgatca cctccggcac tgttgacata ccaactgcgt
    10921 ctgctccgat gatccggaag aacctgaatt cggcaggagt ctcaaatgtc ggacctgtgg
    10981 ttgagagata gactcccctg ctgacaggta tattcagctc tgaagcaatt gtaattgcct
    11041 tctctatcag ctttttgtca tatggctggc tcatatccgg gaaacgggga ccgatgcggt
    11101 catcatttgg gccgataagg ggattgtcaa caaggcatat atgatcatct attatcatga
    11161 tatcgccaat cctgaagtcc gggttgactc ctcctgctgc attggagagg aacagtgccc
    11221 tgattcccag ctccttcatg actctgatgg ggaatgtgac ctgttcggag ccgtatccct
    11281 cgtaatagtg gaaacggccc ttcatggcaa ccaccttttt accgccgaaa tcgccataga
    11341 tgagcttgcc ggcatggccc ttgaccgtag aaacgggaaa atcaggaata tcctcatagg
    11401 ggatttcaac ctggatgtca atctctgccg tcagaccgcc caatcctgtt cctaaaacaa
    11461 ttccggcatc aggatccttc atcccttttg aaaccaggaa ctcagaagtt ttgataatct
    11521 tttccagcat aagagtaaac tttatttaga aatagttttt catcttcaat gaaatgcacc
    11581 ctgacgggca ggtaatacaa cgcctgcctg acatggtctg caatattagt gatttctttt
    11641 aatctgacgc catccttggc agttgtgaca attaatttat ccggaccgct caatgaatca
    11701 tatgccgagc ttattctgcc gatatcagcc tgtgtgtaag cgtggtgatc agggaaggca
    11761 ataccggtaa cctgacctgc aatgctctca aggtactctt tcagtggggc cgggttagct
    11821 atcccggtca caagcaggac ggaggtggtt tgccgtattt caccggtact attgctgcag
    11881 aggtggaggg gttgcccgta tgcgagtgaa gtgaagaaga ttgtttcgca tgcacctgct
    11941 gccatcaact ctctcctgat ctcctctctg gcagagggtg atgctacccc cggggttttt
    12001 gtaactatca ccgcatcagc cctattaaca gccctgatat tctcacgcag cattcccagg
    12061 ggcagcagac ggtcacgggt catcagcctg gtccagtctg tcagtatgat attcattccg
    12121 gctctgacgg ccctgtgctg gaagccatca tcgaggatca ccaccctgat gccgggctct
    12181 tttctcatga tctccctgat gccgtttacc ctgtcacggt caacaaagac ctgtgcctgc
    12241 ggaaggttct gtgccatgag cagcggctca tctcccgaga gggcggtggt gctgtcagcg
    12301 gtcactttca ggaatccacg gcttttacgc aggtacccgc ggctgagtat cgccactgag
    12361 gctatagagg agagcttttc agcgaggtaa ataacatgag ttgtcttgcc tgtacctcct
    12421 gcagttatat tccccacaca gataaccggc aggctgaatg aatgactgcg gaatattcca
    12481 aggtcataga gccggttcct caaggccacg gccatgccgt agatctttga agcggcccac
    12541 aggaagagcc tcccgggttt tatcatccta acggatttcc aggtaatagg gcattgctgc
    12601 ctgtattcct ccggaggaga tgatctcctt cagatcaagg taataccttg aaacctctcc
    12661 cagttccctg cggatggcct tcatcctgat gtcaccggaa agctgcttca gcctggtgta
    12721 aaaatctgat gtttccggct tttctatggt cctgtactct tccagtccgg caagtgaggc
    12781 agcttcactg attgcatcac ggagccctcc gaaagagtca acaagaccga tgggaattgc
    12841 atcggcgccg ctccatacat ggccctgtcc aatgctgtca actgcagtct ggctcatctc
    12901 cctgccttcg gcaacaacac cggtgaaggt gctgtatgtc cgttccacac tgccctgaat
    12961 ggcattcctt tcaaatgcgc tgagcgggcg ggtcagcgag ggtgagtcag agtatgcgtt
    13021 tgtacgtacc acatcatagc ttattcccag tttcttgtcg aggagtgtct ttgcgttggg
    13081 gataaggccg aagactccaa ttgatccggt tatggtaact ggcgatgcaa ttatcctgcg
    13141 cgcggcggcg gagatataat atccgccact ggcggcatag ttgcccatgg ataccacaac
    13201 aggcttgact ttatcagtca gctctacctc ccgccatatc tggtcggagg ctatggcgtt
    13261 gccgccaggt gagttgaccc ggaagacgat tgcctttaca gttgagtcaa gccggagctt
    13321 gcgcagctcc tccgcatacc tgttgccacc tatgtttgta tcattaccgt cacccatgac
    13381 tatgctgccc tcggcaaaga gaacagctat cttatctttc cctgttgccg cgctgtgctt
    13441 cacggtaaca ttggaatact ttgacataga gacataatta atcttcttgc cactgtcaat
    13501 acctgctgca ctcctgatac tgtcttcgag ctgatcacgg taaatggtgc cgtcaatcag
    13561 ccctgcggtt gtcataagag cagggtcata ccctgcaagc gaatcggcaa ggttcattat
    13621 cctttcggcg ggtatgccgc gggaatcaga tatcgacttc accacatgtt cccatatcga
    13681 gcccacgtac ctggttatct ggaggcggtt ctcgtcgctc agcctgtcga gtatgaaggg
    13741 ttccacggca cctttgaatt tgccatgtct tataacctgg acctcaacgc ccagcttttc
    13801 cagggcatcc ttgtagaaag tgacttcagc ggccagcccc ttgaagtcaa acattgaggt
    13861 ggggtttatc cagatggcgt cagcggcagt tgagatgaaa tagctctcct gcatcagcat
    13921 gtagtctgaa taggaataga caaatttccc gctcttttta aattccagca gggcggtgcg
    13981 cagttcgtca gccgttgccc atccggaaag catggtgccg ttttcgatga gaagaccgct
    14041 caccttctca tcttcagctg cctttttcag gtttttgagt atctcgttca gaccgggagt
    14101 ctgggtaaaa gacctggtca tggggtcaat cattgtaaac ggattgttct gtgtacggtc
    14161 tggaatgagc gcccctgttt tcattaccag tacggttttt tcgggaattg ttgccggttt
    14221 ttttcctgct gctgccatcc cggagagggc tgagagcata accacgaaga agaggaggct
    14281 ggtgattatg agtcctgcaa tcacagccag aagcatttta aggaaggttt tcatctctgt
    14341 aaagttgatt taagtgacca aattataaaa aaaacaggca tctttataat gtcttaacgg
    14401 gatgtaatga aattgtcact ctgttcagag gtcatagcgg gccttggaag caatctggga
    14461 gagaggcatg ccgcccttca gcgggcgctg gcgctgctca gggaggaggc aggagagatt
    14521 accgcactgt cttctgtctg ggagactgag ccatggggtt ttgatgccga cgagcagttt
    14581 ctgaacatgg tggccgttat caggacgttc cacagtccgg tgcagctcat tcagcttttc
    14641 aggtcaattg aggggcgcat ggggcgaagg cggagcgggg gcggcaggta cgagtcgcgc
    14701 gttattgacc ttgacattct cttatgggag gagcgtgtca tatccattcc cgggctggag
    14761 gttccccatc cgaagatagc tgacaggagg ttcgtcctga aaccgctctc ggaagtggct
    14821 cctgcagccg ttcacccggt aacggggctg acggtggcag agatgctgca gctctgtgcc
    14881 gacaattctg atgtaaggct ggctgcccgt cagctttaag gcatatacgg gaacaagccg
    14941 gcaccctgcc ggtgcaggac cgtatgcctg ccggttctac agcgccccgc tgtcagcaaa
    15001 actgtaataa tccttcccgg ccacgataat gtggtcaagc agctggatat ccatcagtgc
    15061 accagcctcc tttatcttca gggtgatgcg gatgtcagag tcgctggggc tggtgttccc
    15121 cgaagggtgg ttatggcaga tgataagccc ggatgcgagt gtctcaattg cttttttcat
    15181 gactatgcgt acgtcggtga ccgtaccgct tactcctccc tggctgattt tcatcctgcc
    15241 tgtcacccgg tttgccctgt tgaggaaaag aatccagaac tcctcgtgag aaaggtcttc
    15301 catcaggggg ctgaaaatac tgaacacatc ggctgatgag cgtatctgcg gattatcagc
    15361 ggcttcagcc agtttccgcc gccggcccag ttcaagggct gcagctatgg tgaccgcctt
    15421 ggcttcaccc atccctttga ttttcttgat ctcaccggcg ctgagtctcc ccagctggct
    15481 gagtgagttg ccggccatag ccagcagttc gcgtgccagg tccagagctg agttattcct
    15541 ggtacctgat cttatgagaa tggctaacag ttcagcatcg ctgagacttg cagggccctt
    15601 gttccagagc cgttcacggg gcctgtcctc gacagcccag tctgtaatct tcattgacat
    15661 gcagcaaaac aatttatccc gctgcaatat aaaaaaaaca gccgtcaccc ggaagtgatg
    15721 gctgtgaaat atgtggaaca gaaccctatt tcaggctctg agcgtgctta gtaatagaag
    15781 acttgaggtt ggaagccttg ttcttgtgga tgacgttttt ctttgccagc ttgtcaagca
    15841 tggcattgag ttcgggaagc atcttttctg cctctgcttt cccggtggtg ttgcgcatcc
    15901 tcttgagtgc gttacgcatc gtgcgggcgt agtacttgtt gtctgccttc cttgtctcag
    15961 tctttcttac ggttttgagt gccgacttat ggttagccat tttgttgata attgtaattt
    16021 ggtagtccat aggggaatcg aacccctgtt accaggatga aaacctgacg tcctaaccac
    16081 tagacgaatg gaccttaaag aacttaaaaa agcggatgca aagatggcat ctatttttca
    16141 atctgcaaaa taatatgcag aaaaaatgtc tcttattctg accggctcca aaatgacatt
    16201 gaaaggtatt ctcagaagat gtaccttact gagaatccgc cgttccagta gaagaaaccc
    16261 ggtccgctgg tgacctgtaa tgatgggcct actccaaagc cgaggttcag tggtatgtca
    16321 tcaaaggtgt actccagacc tattattccg tcaaaaccga taagtatccc ggtattatat
    16381 tcatccttcc agtaatcatt ccagaatccg acatgtgctc cggggccgac gaaccagtcg
    16441 aggttgggag cccagttggt aggattctgc cactggtaga atccggttac ggcaaagttg
    16501 tgaccccagt aacctgttcc tgcaatcagt tcaagagcgt tggtgctgtt gatgaatgtc
    16561 ttgaaggtaa tgcctgaagg gtcccctaac ctgaggccga tggctgattt ataactctga
    16621 gcctgcactc cgaaagtcag tgctgccagt accagaatga cagtcagtgt tcttttcatc
    16681 atattcatgt ttaagttgtt ttggacttgc aaagatatat ttatctgtca gattagaata
    16741 agttttgttt atttaaagag caacagactg agcgccatca ccgccatacc tgatacaagg
    16801 ccatagatag aaaggtggtg ctcgccgtat ttctctgctg ccggaagcaa ttcgtcaagt
    16861 gagatgaata ccatgattcc ggcaacgaat gaaaagagta tgcccataag tacgtctgac
    16921 attaccggca gcagcagcag ataacctgcc agggccccta ccggttcggc aaggccgctc
    16981 agaaaagaga gcctgaatgc ttttcgcttg ctgcctgtgg cataatagac cggcactgat
    17041 atggcgatgc cctcagggat attgtgtatt gccactgcca ccgcaattgc tatacctatc
    17101 tgggggtctg agagtgccgc agtgaatgtt gccagccctt cgggaaagtt gtgtatccct
    17161 atggctattg cagtcattgc tcccatgcgt agcagcctgc cgctttgcct ttcctcttcg
    17221 cctccttcta tctttttgaa ctcatggggg ttttcctgtg aaggtatgag tctgtcaatg
    17281 atgcctatga gggctatacc ggcaaagaag gcaattacgg ctgcccacca tccctggcgt
    17341 atgcccattg cttctgtcag aacctcgcgt cccttgacaa agatctcgac aaaagccacg
    17401 tatatcatta cacctgccga gaagcccagg ctaatggtca gaaattttac gttggtcttc
    17461 tttgcgaaaa aggcgatcgt actgcccacg ccagtggaga gcccggcaaa cagagtaagc
    17521 aggaatgcaa aaagtactgt gttgaagtcg gggttcattg ctgtctcagc attgctttat
    17581 cacaggtcaa aagtaccaaa attggttatc ttgcatccgc taaagagcag taaaatgaaa
    17641 ttatctcttt tcaacatttc aaatttccgg gttgacggcg gggccatgtt cggggtgatc
    17701 ccgaaggccc tctggagccg gcagacagag tcagatgagc tgaataccat agtgatgtcg
    17761 ctcacctcac ctgtcattga gaccgaaggg agggtcatac ttgttgatgc cgggtggggc
    17821 gacaagcagg acgagaaatt ctttcgttac acatatcttc acggcgggtc agggcttgcc
    17881 ggcggcctgg cggaatgcgg ttacactcct gatgatatta cggatgtgat aatcacccat
    17941 ctgcatgccg accattgcgg aggctgtttt ataaattctc ccggaggcgg cacagtgcct
    18001 tttttcccca atgccacctg gcacataagc cgcgcccagt gggaatgggc aaacgaatcg
    18061 aatctccgcg aggaggatgc ttttcttccg gagaatatca ggccattcgg cgaaagcgga
    18121 aagctgaact ttattgagga acccggcgaa ctttaccggg gagtgtctct gaggatatgc
    18181 tacggccata ccccgggaat gctaataccc attataagct gttatgggag gacgatggtt
    18241 tacaccggag accttatccc cacggttgcg catatcccgt tgctgtggaa catggcttat
    18301 gacctccttc cgctcgtaac catagccgaa aaacaggaga ttctgcagga ggccctgcag
    18361 aatgactggt tgctgttgtt ccagcatgat ccggctcatg aatgctgctc actttcagaa
    18421 acacctaaag ggatcagggc gggaacaagg ggaaggctgt ccgattttat ctgaatgaaa
    18481 ttattttgat gaggaagagc cccgtatcac caggtcggag gtgattatct ttttctgaac
    18541 aggaccgtgg cggttctcga tgcgatcaat cagcagcctt gcagccatct gtcccatctc
    18601 aaagccct
//
LOCUS       DAOPBK010000028        18526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3096_length_18526_cov_4.832927, whole genome shotgun sequence.
ACCESSION   DAOPBK010000028 DAOPBK010000000
VERSION     DAOPBK010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18526
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3096_length_18526_cov_4.832927"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="PLA17_02870"
     CDS             <1..1290
                     /locus_tag="PLA17_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPK84383.1"
                     /translation="HTGAMASPDTAVDALLRKAGIVRCYSRTDLAIVAALFTYKPLKG
                     KRIAIVTHAGGPAVMLTDILSEGGMEIPHLTGPAADRLLEKLFAGSSVANPIDFLATG
                     TAEQLGYIIDACENDFDEVDAIAVIFGSPGLFPVDDVYNLLHEKIRSCKKPVYPIMPS
                     VINVKREIAEFIARGNAIFTDEVFFGRALCMVNATPPPAVPESKGPHNELKVIREVIE
                     SASDGYLPPEKVNMLLKAAGIETVPESVVTTAAAAVRAAGAMGYPVVMKVVGPVHKSD
                     IGGVVLNIDSEERVRDEFARMMTLPEVTGILVQKMISGREIFCGATREDRYGHLVMAG
                     MGGIFIEVFKDVSTALVPVSDQEARSMIRSLRSYKIIRGIRGTAGVNEEAFVRVITSL
                     SDLLAVAPEIAEMDINPLLGTPDNVVAVDARIRVQAK"
     gene            1646..2968
                     /locus_tag="PLA17_02875"
     CDS             1646..2968
                     /locus_tag="PLA17_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPK84384.1"
                     /translation="MKNTPINRETVSNAIVSSGITNLGYASIREIVKLVNVLRRETGV
                     EFIRMEMGVPGLPSAEVGIRAEIEALKHGVASIYPDIEGLPEMKTEASKFVKNFLDVD
                     VKPECCVPTGGAMMGGMASFMVANRNDRNKEGTLFIDPGFPVQKTQTRILGQEFRSFD
                     VYNYRGEKLGPKLESILECGKVSTILYSNPNNPSWICLTDDELRTIGTLARKYDVIVI
                     EDLAYFGMDFRENYSVPGEPPFQPTVAKYCDDYILLISSSKIFSYAGQRIGLLVMSDH
                     LYSRHYPDLLRYYSTEIFGRAMIFGALYALSSGVTHSVQYGFTAILKAVNEGLYNFVD
                     NTREYGEKAAKMKEMFIKNGFEIVYDMDIDRPVADGFYFTISYPGMSGGELLQELLAY
                     GIAAITLNICGSERQEGLRACVSQVQKSQLPLLEERLRLFRENNPVNE"
     gene            3005..3232
                     /locus_tag="PLA17_02880"
     CDS             3005..3232
                     /locus_tag="PLA17_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPK84385.1"
                     /translation="MAKIKGAVVVDRERCKGCEVCVVTCPTGTLALSTEVNSKGYHYS
                     YAASPDTCIGCANCAVVCPDGVITVYKVKVD"
     gene            3253..4320
                     /locus_tag="PLA17_02885"
     CDS             3253..4320
                     /locus_tag="PLA17_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPK84386.1"
                     /translation="MEKELRLMKGNEALAEAVIRAGVDGYFGYPITPQSEVLEYLMEA
                     NPQQTTGMVVLQAESEVAAINMVYGGAACGKKVMTSSSSPGISLKQEGITYIAGAELP
                     CLIVNVVRGGPGLGTIQPAQSDYFQATKGGGHGDYHLLVLAPASVQEMADFVELGFDL
                     AFKYRNPVMILSDGAIGQMMEKVWLKPQKKRSEEILPWATTGKPASRPRNIITSLDLD
                     PERQEKFNQKLIAKYREMEEKEVRFEEFMTEDAEYLFVAYGTSARLCQKALQLARAEG
                     IRAGLLRPITLFPFPKKRLGELAGRVRGMMAVELSAGQMVEDVMLSANGKTKVRHYGR
                     TGGMIPTPEEVVDNLKSFIKE"
     gene            4326..5102
                     /locus_tag="PLA17_02890"
     CDS             4326..5102
                     /locus_tag="PLA17_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPK84387.1"
                     /translation="MAEVTLNDIIRPENMAYKRSGLMTDNVMHYCPGCGHGTAHQALA
                     AVLEEENLQSRTVGITPVGCSVLMYNYIDIDFQEAAHGRAPALATAVKRLMPDRFVFT
                     YQGDGDLAAIGTAETIHTCNRGENILIIFINNGIYGMTGGQMAPTTLPGMKSSTSPYG
                     RDVSLMGAPIKMADIVAMLPGTCYVTRQSVNTAAGVRKFKRALKKAVQYQNENRGTCF
                     IEVVSNCPSGWKMTPVESNKWLEENMFPFYPLGDLKVPEE"
     gene            5177..5722
                     /locus_tag="PLA17_02895"
     CDS             5177..5722
                     /locus_tag="PLA17_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPK84388.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKIMAYSGMMQDMEVSWMPSYGPEMR
                     GGTANVNVILSDERISSPILRSFDTAIILNQQSLDKFERSVKPGGILIYDGNGITRHP
                     ERKDISVYRIDAAEEASKMNMQRMFNMFVLGGFMKVKPILKTEFVLKGLKESLPERYH
                     HLIPMNEKAIERGKEIIKKVS"
     gene            complement(5762..6382)
                     /gene="nadD"
                     /locus_tag="PLA17_02900"
     CDS             complement(5762..6382)
                     /gene="nadD"
                     /locus_tag="PLA17_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPK84389.1"
                     /translation="MTETGLFFGSFNPVHIGHMAIANYITEFSPLREVWFIVSPHNPL
                     KVKESLLSERDRLYMTQLAIGDDARFRASDIEFTLPRPSYTIDTLTYLSEKYPARRFT
                     LIMGEDNLATLHKWKNAEMLVKQYPVVVYPRLFPGRKSSKKLQEILAMASVRHVDAPI
                     MEISGTFIRSAIREGKDVSWFVPAPVWKYIREMHFYEQRGAGVCDV"
     gene            complement(6379..6960)
                     /gene="gmk"
                     /locus_tag="PLA17_02905"
     CDS             complement(6379..6960)
                     /gene="gmk"
                     /locus_tag="PLA17_02905"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPK84390.1"
                     /translation="MCPADNKKVLIISAPSGAGKTTLINHLMGSGFPLLFSVSATSRK
                     PRGREKEGREYYFITAAEFRKRIRNDEFIEWQEVYENHYYGTLRREIERIRDAEKIPL
                     FDVDVMGGMNLKKIFGNDALSIFIMPPSVEELERRLVLRGTDTPEKIAMRVEKASSEI
                     LLAEHFDRVVINDDLEQARIDIVNIVRDFVEGP"
     gene            complement(6965..7840)
                     /locus_tag="PLA17_02910"
     CDS             complement(6965..7840)
                     /locus_tag="PLA17_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPK84391.1"
                     /translation="MIRSMTGYGKAMLETPQRKITVEIKSLNSKQADISTRFPWLCKE
                     KELEMRNMVIRKLNRGKIDLSVSFDAMESEQVPVINRNNVKNYFSQLKEISNEMGISD
                     DTGLLAIVMRLPETLRTEKAELSDEEWGRISGLIDEALAMTDLYRLEEGKAMENDLAK
                     SVAAILSYLENLEPMEGDRISRMREKLTALLTEAVGSENVDRNRFEQELIFYLEKLDI
                     NEEKVRLRKHCDYFLETMKEEGNHGKKLNFISQEMGREINTIGSKVNDAAMQKLVVMM
                     KDELEKIKEQTLNIL"
     gene            complement(7920..9299)
                     /locus_tag="PLA17_02915"
     CDS             complement(7920..9299)
                     /locus_tag="PLA17_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPK84392.1"
                     /translation="MNLPYCEPYRIKTVESIRQSTRQEREEWVKNASYNLFNLRSEQV
                     FIDLLTDSGTGAMSDRQWGAIMTGDESYAGASSYYTLKETIRDITGFEHFLPTHQGRA
                     AENVLFSVLVKEGDVIPGNAHFDTTKGHIEFRKAKAVDCTVSYARDIEVIDPFKGNVD
                     LERLEQVIRETPREKLGCIVMTVTNNTAGGQPVSMANLRGVRELADRYGVLVVMDSAR
                     FAENAYFIKRREEGYAGKSIREIVREMFSYADAMTMSSKKDAIVNMGGFIAMKDPELF
                     RKASTFNIIFEGYITYGGMSGRDMNALAQGLREGTELDYLEARIGQVKRLGEKIHEAG
                     IPVQLPIGGHAVFIDAGKFFPHIPASEFPAQRLAVEIYIEGGIRGTEIGTLLADRDPV
                     TGENRYPELELVRLAIPRRVYTDNHMDYVAAAVQNVWERRDQVRRGFAIKWEAPIMRH
                     FTVELELPG"
     gene            9729..11639
                     /locus_tag="PLA17_02920"
     CDS             9729..11639
                     /locus_tag="PLA17_02920"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPK84393.1"
                     /translation="MKPGTIKILLFFLCLALTPVLIAQVIDAPVIPEEPEEEADTLAV
                     AQPEPRKIKAAPVPGINGPQDIKLDREMSLRFLERFNSSPAMWKRSNDPLREAIRNLI
                     WLASRPPEDTVISFLSGYPFDRIKVPAESYYLFDSIRIILPILQPDTVHSDSVAASRV
                     DEVFIRTGNKVEKVRLTPDSRPVMKSDTLKLNDSVYVLMNDFIPARLPQSTNDTIMLV
                     ITDTLPEVTLTRTDLPFRYLRNPYVSDSLEAAVSSLIGYLEQRDSTRIRFVGDGARGA
                     DVWLNSLSDNMVRFWLPDGQGDSITVWIGNPVRNTFSLRAEEGVLFRKQAWYDRHADT
                     RVNVITAQEEALRRVALSKIKPEIWKFKTDISYLLSQGVVSNWAKGGENNISSVLDIT
                     TTLNNDRKLSKVISTSTGRFALGFQASGKYGGIRKNLDILEINSKINHKAFGKFDLSG
                     IFQFKSQFLPGYTYPNDTTSIMVSKFFNPATFILGYGLEYKPGKNTSISFSPFSYKGT
                     FVPDTANINQAKYGIPADKWSKNELGAYLTVNSKIVLFEKINMSNRLQLFSNFISKPQ
                     NIDVEWEMISTMPLNWFTDLRINVHLVYDDDTMLPVYKRGEPVLDTDGKQKKAPMVQF
                     KELLGVSFVFKF"
     gene            complement(11636..12508)
                     /locus_tag="PLA17_02925"
     CDS             complement(11636..12508)
                     /locus_tag="PLA17_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HPK84394.1"
                     /translation="MKKLLGRTHDYLSLIRFSHTIFAMPFALAGYFIGATRPGYGFTL
                     KTFLLVLACMVFARSAAMGFNRWADMYFDSLNPRTAVREIPSGKITPRQALLFVTVSS
                     LLFILSAALLNKLTFILSPVALIIVLGYSYTKRFTPLCHLVLGLGLSLAPIGAYLAVT
                     GSFALLPLLYSFIVLTWVSGFDIIYSLQDDQFDRDTGLYSIPAVMSRGKALAISVVLH
                     LITAALVVVAGVYGQAGFIYWTGAAIFTGLLIFQHMIVRPDDLSRVNLAFGTTNGISG
                     VIFGLAVVLDLLLL"
     gene            complement(12505..14955)
                     /gene="pheT"
                     /locus_tag="PLA17_02930"
     CDS             complement(12505..14955)
                     /gene="pheT"
                     /locus_tag="PLA17_02930"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPK84395.1"
                     /translation="MKISYNWLRDYLDFDAQPDELSSVLTSLGLEVEDVEEWVSVKGG
                     MKGVVIGRVLTCEKHPDADRLSVTTVDTGGPDPLHIVCGAPNVAAGQVVAVALPGTMV
                     FHGDEQFEIKKSKIRGQLSEGMICAEDELGLGADHEGIMVLDSNARIGMPAAEYFGIT
                     TDTVFEIGLTPNRIDCSSHFGVARDLAAFYNLRRPVRAKLPEISGFRKDRESGAIEVT
                     VEAPEACIRYSGLTVRGIRVEPSPAWLQTRLRAIGLHPINNIVDITNFVLHETGQPLH
                     AFDAAAITGDIVRVRHLPDKTKFVTLDGVERTLASSDLMICNRDEGMCIAGVFGGLKS
                     GVTEATTDLFLESATFNPVSVRRTSRRHDLHTDASYRFERGTDPEMTIYALKRAAMLV
                     KELAGGEITGDIIDLCPKPVRKAVVKYSLDRMTRLIGKEIPEETVKTILYSLDINITG
                     EEPGVLHLEIPSYRVDVTREADVTEEVLRVYGYDNIGISHEMRSMLSHTEKPDPEKIA
                     SNCSDMLAANGFCEIMCNSLSPAAWFETTGDFDLKSMVRLANPLSSDLNVMRLSLLPG
                     MLNAIAWNINRQNSNLRLFELGYTYARKEGKGTLEITDNFSEKQILALALTGNINEKR
                     WNAPEMSSGFFHLKGYTEMILSRFGITGKELIVTAENRGWFTEGMRYTAGSLEVASFG
                     KVSKKYLAMFDIRQEVFYAEIDRENLIRLISSRTIRFRELPRYPWVRRDLALLVDKSV
                     KFSQIRDIAFRTERNILREVDLFDLYESKEIGTDKKSYAVSFILHDERQTLTEKNIEK
                     TMNALVKAFERELGATLR"
     gene            15079..15855
                     /gene="surE"
                     /locus_tag="PLA17_02935"
     CDS             15079..15855
                     /gene="surE"
                     /locus_tag="PLA17_02935"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPK84396.1"
                     /translation="MKIEENDKLILLTNDDGLYAGGFKTLIRIMEDFGKVVVISTTEG
                     RSAMSQALTVKQPLRVKLLVEDDRKRIYACSGTPADGIKLAVSQLLDRKPDLVVSGIN
                     HGSNASASILYSGTMAAALEGCLYGITSVGFSLNSYSSSADFSGCEEYIRDICTMLLR
                     EPLPTGVCLNVNFPAVPASEIKGMMVARQALGNWQEEFEKRKDPAGKTYYWLTGSFIN
                     HEPEASDTDDWAINNGYVSVVPVTIDMTAYSFMETLKSKL"
     gene            15857..16153
                     /locus_tag="PLA17_02940"
     CDS             15857..16153
                     /locus_tag="PLA17_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84397.1"
                     /translation="MWFRQRFNNVISGVVGGLVMPLLGFLIFFLLTHHGLSMTDYFRK
                     MEVAGNISEIMSVSVFTNVVIYLLFNRLDMLRACRGVLGITLAWAFVVFAINLF"
     gene            16155..17288
                     /gene="lpxB"
                     /locus_tag="PLA17_02945"
     CDS             16155..17288
                     /gene="lpxB"
                     /locus_tag="PLA17_02945"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPK84398.1"
                     /translation="MKYFIIAGEPSGDLHGSNLVKHLLKSDPAADIACWGGELMQGEG
                     ARLLKHYRETAFMGVFEVLVNIRKVKANFSECKRQIMEMKPDVVILIDYPGFNLRMAR
                     FAKEAGLRVFYYISPKFWAWREGRVRKIKRYTDRLYIIFPFEKEFYRKHDFPAIFLGN
                     PLIDHIDAWRATAPDREMIRASLGLDARPVIALLSGSRLQEVSKILPVMAEVSEDFPD
                     YQFVVTAMDHLPARLYEEVSSAYPVRVITGRTYDLLAVAEAALVTSGTATLEAALFDV
                     PQAVCYSTSRLTYRLAKWLIRVRFISLVNLIMDREVVRELIQEGLNSASLHRELEEIV
                     ATGYRHERIKDDYRELRTILGGGGASARVAADMIESLVNKKAG"
     gene            17350..18501
                     /locus_tag="PLA17_02950"
     CDS             17350..18501
                     /locus_tag="PLA17_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPK84399.1"
                     /translation="MKRISLVAILLVLFPLVTFSRISIRIFARSKPSSLIVSMVNGDC
                     MLFDGTAGEVRLSQGETVAVTRYGDRVIYSTLSGIRGVSDSLAFTPAASGTLFTVRAP
                     GSSEPEKSLGGTLTVTSFPGSLRILNITTVEECLPGVVRAEAGRYGPSDYYRAQAVVA
                     RTYIYRNIDRHSLDGYNMCDDVHCQVYPGVVTDSVIVNACRSTSGLVLADRDSIMIEA
                     AFHGNCGGETASSADVWLAGYPYLVSVTDPWCSYSPSSTWRKNIARSEWNSFLRSKGI
                     APGTEGAIYSANAPAAGRITYRTVQGKTLWSEEIRLRFGLRSAYFTLSDAGDSILFNG
                     RGYGHGVGLCQDGAKHMAEKKMSYNRIIGFYYPGTIITDVKNARRPVRP"
BASE COUNT         4586 a         4614 c         4849 g         4477 t
ORIGIN      
        1 cacaccggcg ccatggcgtc acctgacacg gcagtggatg cactgctgcg caaggcaggt
       61 atagtaaggt gttacagccg gacagacctg gctatagtgg ctgccctgtt tacatataaa
      121 ccactcaagg gcaaaagaat agccattgtc acccacgccg gcggaccggc ggtgatgctc
      181 accgatattc tcagcgaggg cggaatggag ataccccacc tgacaggtcc ggcagcagac
      241 cggctgcttg aaaagctctt tgcagggtca tcggtggcaa acccgatcga tttcctggcc
      301 acaggtaccg ccgaacagct gggttatata attgacgcct gtgagaacga ttttgacgag
      361 gtggatgcaa ttgcggtcat cttcggcagc cccggactgt tcccggtgga tgatgtttac
      421 aacctgcttc atgaaaagat ccgcagctgc aaaaaaccgg tttatcccat tatgccgtca
      481 gtcataaacg tgaagaggga gatagccgag ttcatcgcga ggggcaatgc cattttcact
      541 gacgaggtat tctttggcag ggcgctgtgc atggtcaatg caacaccgcc cccggccgtg
      601 ccggaaagca aaggtcccca taatgagctg aaagtgatca gggaggtcat cgagagcgcc
      661 tctgacggtt atctgcctcc ggaaaaagtc aatatgctgc taaaggcagc aggaattgaa
      721 acggtacccg agagtgtagt cactaccgct gcagctgctg tcagggctgc tggcgcgatg
      781 ggttatccgg tggtgatgaa ggtcgtgggc cctgtacata agtcggatat tggcggtgtg
      841 gtacttaaca tcgacagtga ggagagggtc agggatgagt tcgcccgtat gatgactctt
      901 ccggaagtga ccggcatact ggtacagaag atgataagcg gacgcgagat attctgtggc
      961 gctaccagag aggaccgata cggtcacctg gtgatggcgg gcatgggagg aatctttatc
     1021 gaggtcttta aagatgtttc aactgcactt gtgcctgtgt ctgaccagga ggcaaggtct
     1081 atgatacgct ctctcaggtc gtacaagatt atcaggggca tacgcggtac tgccggcgta
     1141 aacgaggagg cattcgtcag ggtgataacc tcactttccg atctcctggc cgtcgcacct
     1201 gagatagccg agatggatat taacccgctg ctgggcaccc cggataatgt ggtggctgtg
     1261 gatgcacgca tccgggtaca ggcaaaatag agcaggcagc agccccgata agtgatgatg
     1321 tggggtgatt gagtttgcca ggtacaggca aaacagagca ggcaattgcc aatagatact
     1381 tgttgccgcc ctctgcagca ccatcctcgg agtcatcctc gcgatgcctg cgggagcagg
     1441 gaagaaggag cagggagtag agaagccggc atgattccat tcaaatagtg ccccggactt
     1501 ttaagaattg ctgatgttag gatttcttcc aactattggc cgggtcatta agcattttaa
     1561 ttcgtaatta tgataaatag catttatcgt tctcttgtaa ttttatatct taggagcaca
     1621 gatgagggcg ctaaataaag aatttatgaa aaataccccc attaatcgtg aaacagtaag
     1681 taatgcgata gtatccagcg ggattacaaa ccttggttac gcaagtatcc gggagatagt
     1741 caagctggtg aatgtgctac gacgcgagac aggtgttgaa ttcattcgca tggagatggg
     1801 tgttccgggc cttccctcgg cagaggtggg tatcagggcc gagattgaag ccctgaaaca
     1861 cggcgttgcc tcaatatacc ctgacataga aggtttgcct gagatgaaaa cggaagcatc
     1921 aaagttcgta aagaattttc ttgatgtaga tgtgaaaccg gaatgctgcg tgccaacagg
     1981 cggagcaatg atgggtggca tggcaagctt catggtggcc aaccgcaatg accgcaacaa
     2041 ggagggaaca ctcttcattg acccgggatt cccggtgcag aaaacccaga cgaggattct
     2101 cggccaggag ttcaggtcat ttgatgtcta caactaccgt ggagaaaagc tgggccccaa
     2161 actggaatca atactggagt gcggaaaggt ttcaacaatc ctctattcaa accccaacaa
     2221 cccttcatgg atctgtctca ctgatgacga actccgtaca ataggcactc ttgccaggaa
     2281 gtatgatgtc atcgtgattg aagacctggc ctactttggc atggatttca gggagaacta
     2341 ttctgttccc ggtgagccgc ccttccagcc aacggtggca aaatactgcg acgactatat
     2401 ccttctgatt tccagctcta agattttcag ctatgccggt cagagaatag gcctcctggt
     2461 aatgtcagac catctctata gccggcatta tccggacctt ctccgctact attcaaccga
     2521 gattttcggc agggcgatga tctttggtgc gctctatgcc ctctcctcgg gagtgaccca
     2581 ctcggtgcag tacggattta ctgccattct caaggcagtc aatgaaggtc tctataactt
     2641 tgtagacaac acgagggaat acggagagaa ggctgcaaaa atgaaggaga tgttcatcaa
     2701 gaacggtttt gaaattgttt atgacatgga cattgacagg cctgtggcag acggatttta
     2761 cttcactatc tcctatccgg gaatgagcgg gggtgaactg cttcaggagc tgctcgcgta
     2821 tggcatcgcc gcaataacac tgaatatatg cggcagcgag aggcaggagg gactcagggc
     2881 ctgtgtgtcg caggtgcaaa aaagccagtt gccgctgctt gaggaacggc tcaggctatt
     2941 cagggaaaac aacccggtaa atgaataaag cttattttta attaagggaa taaattcaaa
     3001 acttatggca aagataaaag gagcagttgt tgttgaccgc gaaaggtgca agggatgcga
     3061 ggtatgcgtt gtaacctgtc ctaccggaac cctcgccctg tcaacagagg taaattcaaa
     3121 gggatatcat tacagttatg cagccagtcc tgacacatgc atcggatgcg ctaactgcgc
     3181 agtcgtgtgt cctgacggcg ttattaccgt ctataaggta aaggttgatt aaaagaaaaa
     3241 taaaatatgt caatggaaaa ggaactgaga ctgatgaagg gcaacgaagc tctcgccgaa
     3301 gcggtgatac gtgccggtgt cgacggttat ttcgggtatc cgatcactcc gcagagtgag
     3361 gtacttgaat atcttatgga agccaatcct cagcaaacca ccgggatggt ggtactccag
     3421 gcggagagtg aggtggcagc catcaatatg gtttacggcg gcgctgcctg tggaaagaag
     3481 gtgatgacct cctcctcatc acccggaata agccttaaac aggaggggat aacctatatc
     3541 gcaggggccg agcttccctg ccttattgtc aatgtggtgc gcggcggccc gggactgggt
     3601 accatacagc cggcacagag cgactacttc caggctacaa agggaggcgg acatggcgac
     3661 tatcacctgc tggtactggc tcccgcatct gtccaggaaa tggctgactt cgtcgaactg
     3721 ggatttgacc tggcattcaa atacagaaac ccggtgatga tcctgtctga cggagccatc
     3781 gggcagatga tggagaaagt gtggctcaag ccgcagaaaa agagatcaga agagatactt
     3841 ccgtgggcaa caaccgggaa gccggcatcg cgcccgagaa atataatcac ctcactggac
     3901 cttgacccgg agcgacagga aaagtttaac cagaagctga ttgccaagta cagggagatg
     3961 gaggagaagg aggtacgttt cgaggaattc atgactgaag atgccgaata tctcttcgtg
     4021 gcatacggaa caagcgccag gctgtgccag aaagcactgc agctggccag ggccgaaggc
     4081 atcagggcag ggctgctgag accgataaca ctcttcccct tcccgaagaa gagactcggt
     4141 gagctcgccg gcagggtcag gggcatgatg gcagtggagc tgagcgccgg ccagatggtt
     4201 gaggacgtga tgctctccgc gaacggaaaa acaaaggtca ggcattacgg aaggacggga
     4261 ggtatgatcc cgactcccga agaggtggta gataacctga aatcattcat taaagaataa
     4321 aagatatggc tgaagtaaca ctcaatgata taatcaggcc cgagaacatg gcctacaaaa
     4381 ggtctggtct gatgaccgat aatgtcatgc actactgccc cggctgcgga cacggtaccg
     4441 ctcaccaggc cctggcagcg gtgctggagg aggaaaacct gcagagtcgc acggtgggaa
     4501 tcactcccgt cggatgctca gtactgatgt acaactatat agatatcgat tttcaggaag
     4561 cggcacacgg aagggcacca gccctggcaa cagctgtcaa aaggctgatg cccgacaggt
     4621 tcgtcttcac ctaccagggt gacggtgacc tggctgccat tggtaccgcg gaaacgattc
     4681 atacctgcaa caggggtgaa aatatcctga tcatctttat caacaacggt atctacggca
     4741 tgaccggagg ccagatggct cccacaacac tgccgggaat gaaatcgtca acatcaccct
     4801 acgggcgcga cgtttcattg atgggtgcac ccatcaagat ggctgatatc gtggccatgc
     4861 tgccgggtac ctgctatgtg acccgtcaga gtgtcaatac agctgccgga gtacgcaagt
     4921 tcaagagagc gctgaaaaag gcagtacagt accagaatga aaacagggga acatgtttca
     4981 tcgaggttgt gtcaaactgc ccctcgggat ggaagatgac acctgtggag tcaaacaagt
     5041 ggctggaaga gaacatgttc ccgttctatc ccctgggtga tttaaaggta cctgaagaat
     5101 aggggcctga tccgggtgaa gcatatttcg ctttgcaccg gaaattgagt ttaatgaatc
     5161 aatattacta cagagcatga cagaagaaat aataattgca ggttttggcg gacagggtgt
     5221 actctcaatg ggtaagataa tggcctattc cggcatgatg caggacatgg aagtgagctg
     5281 gatgccctcc tacggccccg agatgagggg cggcactgcc aacgtcaacg taattctcag
     5341 tgatgaacga atcagttcac caattctcag gagcttcgac acggccatca ttctgaacca
     5401 gcagagcctt gacaagttcg agaggtcggt caagccgggg ggaattctca tttatgacgg
     5461 taacggtatt acgagacatc ccgaaagaaa ggatataagc gtgtaccgga ttgacgcagc
     5521 ggaagaggct tcgaagatga acatgcagag gatgtttaat atgtttgtcc ttggcgggtt
     5581 catgaaggtg aagccgatac tgaaaaccga atttgtcctc aagggactga aagaatcact
     5641 tcccgagcgc taccaccatc tcatccccat gaatgagaaa gctattgagc ggggtaagga
     5701 gattattaaa aaggtttcgt gatttgaaat ttgcaattta cgattcagat ttgcgattcc
     5761 atcagacatc gcaaactcca gctcctctct gctcgtaaaa atgcatctcc cggatgtact
     5821 tccagacggg agcgggaacg aaccatgaga catccttccc ttcgcggata gcgctgcgga
     5881 tgaaggtgcc cgagatttcc atgatgggtg cgtcaacatg ccggaccgaa gccattgcca
     5941 gtatctcctg cagctttttg ctgcttttcc ggccagggaa gagcctcgga tagaccacaa
     6001 caggatactg ctttaccagc atttcagcat tcttccactt atgaagggtg gcgagattat
     6061 cctcgcccat gatcagcgtg aaccgccgag ccgggtactt ctcggaaaga tatgtcaggg
     6121 tgtcaattgt gtatgaaggc ctgggcagag tgaactcaat gtctgaagcc ctgaaacggg
     6181 catcatcacc tattgccagc tgagtcatat agagcctgtc acgctcactg agaagcgact
     6241 ccttcacctt cagcggattg tgcggactga caatgaacca tacctccctg agaggggaga
     6301 attcggtgat atagttggca atggccatgt gacctatatg caccgggttg aatgatccga
     6361 agaacagtcc tgtctcagtc atggcccttc cacaaagtcc ctcacaatgt ttactatatc
     6421 aatacgggct tgctcaaggt cgtcattaat gaccaccctg tcgaaatgtt cagccagcag
     6481 tatctccgaa gaagctttct ccaccctcat tgcaatcttt tccggtgtgt cagttccgcg
     6541 cagcacaagc ctccgttcca gttcctccac agaagggggc atgatgaaga ttgacagggc
     6601 atcattgcca aagatcttct tcaggttcat tcctcccatt acatccacgt caaagagcgg
     6661 aattttttct gcatcgcgta tgcgctcaat ctccctgcga agtgttccgt aataatggtt
     6721 ttcataaacc tcctgccact ctatgaattc atcattccgg attctttttc tgaattccgc
     6781 agcagtgatg aagtaatact ccctgccctc tttctccctg ccgcgcggct tcctgctggt
     6841 ggcggaaact gaaaagagga gggggaagcc tgagcccatg agatggttta tcagtgtcgt
     6901 tttaccggca cctgagggag cggaaatgat aagtaccttt ttgttgtcgg ccgggcacat
     6961 ctgtttaaag gatattaagt gtctgttcct tgatcttctc cagctcgtcc ttcatcatca
     7021 ccaccagttt ctgcatggca gcatcattta ccttggagcc gatggtattg atctcgcggc
     7081 ccatctcctg cgagatgaag ttgagcttct tcccgtggtt gccttcttct ttcatcgttt
     7141 ccaggaagta gtcgcagtgt ttacgcagcc tgaccttttc ctcgttgatg tcaagttttt
     7201 caaggtagaa aataagctcc tgctcaaacc tgttcctgtc aacgttttct gatcctacag
     7261 cctctgtcag caatgctgta agcttttcgc gcattcgtga gatacggtca ccctccatgg
     7321 gttccaggtt ttcaagatag ctgaggatgg cagcaaccga tttggcaaga tcgttttcca
     7381 tggcttttcc ctcttccagc cggtagaggt cggtcattgc cagggcttca tcgatgagtc
     7441 cggaaatcct gccccactct tcatcagaga gctccgcctt ctccgtgcgc agtgtctcgg
     7501 gcaggcgcat cactatggcc agaagtccgg tgtcatcact gatccccatt tcattgctga
     7561 tctccttcag ctggctgaag tagttcttta cgttgtttct gttgatcacc ggcacctgct
     7621 cgctctccat ggcgtcaaag cttaccgaga ggtcgatctt gcccctgttg agcttcctga
     7681 tgaccatgtt acgcatctcc agctccttct ccttgcatag ccaggggaat ctggtgctga
     7741 tatctgcctg cttgctgttg agtgatttga tctcaacagt gatctttctt tgcggagtct
     7801 ccagcatggc tttgccatag ccggtcatcg atcttatcat cgtgttgcgg taattacagg
     7861 gttaaagata ggtttttatt cgcagcggaa gtgatctggt ccggtggtga agctgtaacc
     7921 taacccggca gctcgagctc cacggtgaag tgcctcatga ttggcgcctc ccactttatt
     7981 gcaaatcctc ttctgacctg gtctcttctc tcccagacgt tctgcaccgc tgcagccacg
     8041 tagtccatgt ggttgtcagt gtaaaccctt cgcggtattg ccagcctgac cagctcgagc
     8101 tccggatacc tgttttcacc tgtgacgggg tcacggtcgg caagcagtgt acctatctcg
     8161 gttccccgga tgcctccctc aatgtatatc tccaccgcca gcctctgggc cgggaactca
     8221 gaagcaggga tgtgggggaa gaactttcct gcatcgataa agacggcatg gccaccgatg
     8281 ggtagctgca ccgggatgcc ggcctcgtga atcttttctc ccagcctttt tacctggcct
     8341 atacgtgcct caaggtagtc taactccgtt ccttcgcgaa gcccctgtgc aagtgcgttc
     8401 atgtcacggc ctgacatgcc gccgtaggtg atatagccct cgaatatgat gttgaaggtg
     8461 gaggccttcc tgaacagttc agggtctttc atggcaatga acccgcccat gttgactatg
     8521 gcatccttct tcgaactcat ggtcatggca tcagcatatg agaacatctc gcggactatc
     8581 tccctgatac tcttgccggc ataaccctct tcgcgtctct tgatgaagta ggcgttctct
     8641 gcaaagcggg cagagtccat caccacaagc actccatacc tgtcagccag ttccctcact
     8701 ccccgcaggt tggccatgct taccggctgg ccgccggcag tgttgttggt tacggtcatt
     8761 actatgcatc ccagcttttc ccgcggagtc tccctgataa cctgttccag tctttcaagg
     8821 tcaacatttc ccttgaacgg atcaatcacc tcaatgtccc tggcgtatga gacggtacag
     8881 tctaccgctt ttgccttgcg gaactctata tggcccttgg tggtgtcaaa gtgggcattg
     8941 ccggggatca catcaccctc ctttaccagc acggagaaga gtacattttc agcagccctt
     9001 ccctggtgcg tggggaggaa atgctcaaaa ccggtgatat ccctgattgt ctctttaagg
     9061 gtgtagtagg atgaggctcc ggcatagctc tcgtcgcccg tcatgatggc gccccactgc
     9121 ctgtcgctca tcgcgccggt acccgagtct gtcaggagat caatgaatac ctgctctgaa
     9181 cgcaggttaa acagattgta tgaggcattc tttacccact cctcccgctc ctggcgggtg
     9241 ctctggcgga tcgactcaac tgtctttatc ctgtaaggtt cacaatatgg taagttcatg
     9301 gttgttaatg atttataatt ctgttaaggt ctatgattat ctgttgttta ttgtctgttt
     9361 gctgtaattc ttacaatctt cagaagcatt ggcttcagct gatgtccggg ttttattgtc
     9421 tgtttgctgc aattcttact ggtctgcgac taatattcaa cgtatgctct ccatctgcaa
     9481 tcagatgcca ttgttgtctg ttaaaaccaa cgcttaagaa aatttaacaa ttaacggaag
     9541 ataattgctc caaaggaagc aaccatgccg gtcagaatgc gtctctgttc ttgatgttac
     9601 gataattcag cttcctgttt gtgatgctgt agaagcggga tttcatggaa ttaatgtaat
     9661 tcggctattt ttgcaaatat aaccattttg cttttggggg acaatccaga actgttaaac
     9721 gttttgaaat gaaaccggga accataaaga ttcttttgtt ttttctttgc cttgccttga
     9781 caccggtact gattgcacag gttattgatg caccggtgat tcccgaggag ccggaggagg
     9841 aggctgatac actggcagtg gcacaaccgg agcccagaaa gattaaggct gctcccgtgc
     9901 cgggtatcaa cggtccgcag gatataaaac ttgacaggga gatgtcactc aggttccttg
     9961 agcgtttcaa ctcctcaccg gcaatgtgga aaagaagcaa tgaccctctt cgtgaagcca
    10021 tcagaaacct catctggctt gcttcaaggc ctccggaaga tacagtaatc agtttccttt
    10081 ccggttatcc gtttgacagg ataaaggtac cggctgaaag ctattacctg tttgactcga
    10141 taaggatcat tcttcccatc cttcagcctg acacagtgca ctctgacagt gttgctgcct
    10201 cacgcgttga tgaggtgttc atcagaaccg gcaacaaggt ggaaaaggta aggctgaccc
    10261 ctgactccag accggtaatg aaaagtgata cactcaaact gaatgactct gtttacgtcc
    10321 tgatgaacga tttcataccg gcgcggctgc ctcaaagcac caatgacaca attatgctgg
    10381 taatcaccga tacccttccg gaagtcaccc ttacccgcac tgatctccct ttcagatatc
    10441 tcaggaatcc gtatgtcagt gattcactgg aagctgctgt cagttcgctc atcggttatc
    10501 tcgaacagcg tgattccacg aggatacgct ttgtcggcga cggggcaaga ggcgctgatg
    10561 tatggctcaa cagcctctct gacaacatgg tacggttctg gctgccggac ggtcagggcg
    10621 attcgataac ggtttggata ggcaatccgg tgcgcaacac cttctcactc cgtgcagagg
    10681 agggtgtgct cttcagaaaa caggcctggt atgacaggca tgccgacacc cgcgtgaatg
    10741 tcataacagc gcaggaggag gcgctgagga gagttgcact gagcaagata aaacctgaga
    10801 tatggaagtt caagacagac atctcatacc tgctctcaca gggtgtggtc tctaactggg
    10861 caaagggggg agaaaacaac atctcctcag tgcttgacat aaccacaacc ctgaacaacg
    10921 acaggaagct ttcaaaggtg atctcaacca gcacgggcag attcgcgctg ggattccagg
    10981 cttccggcaa atatggcggc ataaggaaaa accttgatat cctcgagatt aactccaaaa
    11041 tcaatcacaa ggcattcggc aaattcgacc tctcggggat cttccagttc aagtcgcagt
    11101 tcctgccggg ttacacatac ccgaatgaca ccacctcaat aatggtctcc aagtttttca
    11161 atccggccac ctttattctg ggttacggcc ttgaatacaa acccggcaaa aacacatcga
    11221 taagcttctc cccattttca tataagggca catttgttcc tgacaccgca aacatcaacc
    11281 aggccaaata tggtattcct gctgacaagt ggtcaaagaa cgagctgggt gcatacctga
    11341 ccgtcaacag caaaattgtg ctcttcgaaa agataaacat gagcaacagg ttacagctct
    11401 tcagcaattt catttcgaag cctcagaata tagacgttga gtgggagatg atatccacga
    11461 tgccgcttaa ctggttcact gacctcagga taaacgttca cctggtttat gatgatgaca
    11521 ccatgctccc ggtatataaa aggggagagc cggtgcttga caccgatggc aagcagaaga
    11581 aggctcccat ggtgcagttc aaggagttgc ttggcgtcag cttcgtcttc aagttttaaa
    11641 ggaggagcag gtcaagtacc actgcaagcc cgaaaatgac tccggatatt ccgttggttg
    11701 tgccgaaggc aaggtttacc ctgctaaggt catcaggcct gacaatcatg tgctgaaaaa
    11761 tgagcaagcc ggtgaatatg gctgcacctg tccagtatat gaatcctgcc tggccatata
    11821 ctcctgccac caccaccagg gctgccgtaa tcaggtgaag cacaactgaa atggccagcg
    11881 ctttgccgcg cgacatcacc gccgggattg aatagagtcc tgtgtcacgg tcaaactggt
    11941 catcctgaag ggaatagatt atgtcaaaac cactcaccca tgtcagcacg atgaaggagt
    12001 agaggagcgg aagcagggca aaagagcctg tcaccgccag gtatgctcct atgggagcaa
    12061 gggagagccc cagtcccagc accaggtggc acagcggggt gaaccgcttg gtgtaactgt
    12121 acccgagaac aattatcagg gccacaggag agagtataaa ggtcagtttg ttgagcaggg
    12181 cggcggatag aatgaataaa agtgaactga ctgttacaaa cagcagggcc tggcggggag
    12241 tgatcttccc tgacgggatc tcacgcactg ccgtccgtgg attgagtgaa tcaaaataca
    12301 tgtcagccca ccggttgaaa cccatggccg cacttcgtgc aaagaccatg catgccagaa
    12361 caagcaggaa ggtcttcagt gtgaagccgt accccggccg ggttgcccct ataaagtaac
    12421 cggcaagagc aaagggcatc gcaaagatag tgtgactgaa cctgatgagc gacaggtaat
    12481 cgtgtgttct gcccaaaagc tttttcatct cagcgtggca cctaactcac gctcaaaggc
    12541 tttgaccagg gcgttcatgg tcttctcaat attcttttcg gtgagcgtct gtctctcatc
    12601 atgtagtatg aagctgacgg cgtacgactt cttgtctgtc cctatctctt tactctcata
    12661 aagatcgaag aggtctacct ccctgagtat gtttctttca gtcctgaagg caatatcacg
    12721 tatctgactg aattttactg acttgtcaac aagcagggcc aggtcacggc gcacccacgg
    12781 gtatctgggc aattccctga aacggatggt ccggctgctg ataagcctga taaggttctc
    12841 ccggtcgatc tcggcgtaaa atacctcctg cctgatatca aacatagcca ggtatttttt
    12901 tgacactttg ccgaaggagg ctacttcaag acttccggca gtgtatctca tcccttcggt
    12961 gaaccagccc cggttttctg cagttacaat gagctccttg ccggtgatgc cgaaacgtga
    13021 aaggatcatc tccgtataac ccttgaggtg aaagaatcct gatgacattt caggggcatt
    13081 ccaccgtttt tcgtttatat tcccggtcag tgcaagggca agtatctgtt tttcgctgaa
    13141 gttgtcagtg atctccaggg tgccttttcc ttctttccgg gcgtaggtgt atccgagttc
    13201 aaacagcctc aggttgctgt tctgcctgtt gatgttccag gcaatggcgt tcagcatgcc
    13261 cggcagcagc gagagtcgca tgacgttaag gtcactgctg agcggattgg ccagtctcac
    13321 catcgacttc aagtcaaagt cacccgtagt ttcaaaccag gcagcagggg agagggagtt
    13381 gcacattatc tcgcagaagc cgttggcagc gagcatatct gagcaattgg aggcaatctt
    13441 ttcagggtca ggcttttcag tatgtgacag catggagcgc atctcatggc ttatccctat
    13501 gttgtcataa ccgtaaaccc tgagcacctc ttcagtgaca tcggcctcac gggtgacatc
    13561 aacacggtat gacggtatct caagatgcag gacgccgggt tcttcgccgg tgatattgat
    13621 atccaggctg tagagaatgg ttttgactgt ttcttccggt atttccttcc ctataagtct
    13681 ggtcatccgg tcaagggaat acttcacaac tgcttttctc accggcttgg ggcagagatc
    13741 aatgatatct cctgttatct caccgccggc aagctcctta accagcatgg cagctctctt
    13801 cagggcatag atggtcatct ccggatcagt gcctctctca aacctgtatg atgcgtcagt
    13861 gtgcaggtca tgcctccgcg atgtgcgccg tactgacacc gggttgaagg tggcgctctc
    13921 gaggaaaagg tcggtggtgg cttcggtgac gcctgatttc agtccgccga aaacacctgc
    13981 aatacacatc ccctcgtcac ggttgcatat catcaggtca gacgatgcaa gggtgcgttc
    14041 cactccgtca agggtaacga atttcgtttt gtcaggaagg tgccttaccc tcactatgtc
    14101 tccggtaatg gctgcagcat caaatgcatg cagtggctgt cctgtctcgt ggaggacgaa
    14161 gttggtgatg tcaacgatgt tgttgatggg atgaagtccg attgccctga gcctggtctg
    14221 taaccatgcc ggggaaggtt caaccctgat accgcgcact gtcagtccgg agtaacgtat
    14281 gcatgcttcc ggagcctcta cggtgacctc tatggcacca ctctccctat ctttcctgaa
    14341 ccctgaaatc tcaggcagct ttgcccttac cggtctccgg aggttgtaga aggcagcaag
    14401 atcgcgtgcc accccgaagt gtgagctgca gtcaatcctg ttcggtgtca gccctatctc
    14461 aaagaccgtg tcggtagtaa ttccgaaata ttcagcagca ggcatgccga tgcgtgcgtt
    14521 gctgtcaagc accattatcc cttcatggtc agcgcccagg ccaagctcat cctctgcaca
    14581 gatcattcct tcggacagct ggccgcgtat ctttgatttt ttaatctcaa actgttcatc
    14641 tccgtgaaag accatcgttc ctggcagggc cactgccacc acctgtcctg ctgctacgtt
    14701 cggagccccg catacaatat gcaacgggtc aggtcccccg gtatccactg tggtgacact
    14761 cagcctgtca gcatcagggt gcttttcaca ggtgagtacc ctgccaataa ccactccctt
    14821 cattccgccc tttaccgaaa cccactcttc aacatcctca acctcaaggc ccagtgatgt
    14881 cagaaccgat gacagctcat ccggctgcgc atcaaaatca agataatccc tgagccagtt
    14941 gtaagaaatc ttcatcgcgg tattgttatt aggttttcag aaatgcaaaa gtaaggaaaa
    15001 aaggtttgag gcatcaagca gacgcggcat taaaagcaga gccattatca ttatatttgc
    15061 acttcaacaa aaattcagat gaaaatagaa gaaaacgata agcttattct tttgacaaat
    15121 gatgacggac tctatgcagg tggctttaaa accctcataa ggatcatgga ggacttcggc
    15181 aaggtggtgg taatctcaac caccgagggc cgcagtgcca tgtcacaggc gctgactgtc
    15241 aagcagcccc tgagggtcaa gcttctcgtg gaggatgacc gcaagcggat ctacgcctgc
    15301 agcggcactc ctgcagatgg aatcaaactg gcggtcagcc agctgcttga ccgcaagcct
    15361 gacctggttg tctcaggcat caaccatggc tccaacgcct ccgccagcat actctattca
    15421 ggaacaatgg cagccgctct tgaaggatgt ctgtatggca tcacgtcagt gggattctct
    15481 cttaacagct attcgtcctc agccgatttc tcggggtgtg aagagtatat cagagatata
    15541 tgtacgatgc tccttcgcga gccattgccg acaggtgtat gccttaatgt caacttcccg
    15601 gctgtgccgg cttctgagat aaaagggatg atggtagccc gccaggccct gggtaactgg
    15661 caggaggaat ttgaaaagag aaaagaccct gcgggaaaga cctactactg gctgacaggc
    15721 agttttatca accatgagcc ggaggcatca gatactgacg actgggccat taataacggg
    15781 tatgtctctg tagtgccggt gaccattgac atgactgcct acagctttat ggaaaccctc
    15841 aaatcaaaac tctgatatgt ggttcagaca aaggttcaat aacgttatat caggtgttgt
    15901 gggaggattg gtgatgcctc ttctggggtt cctgatattc tttctgctga cacatcacgg
    15961 gctttcaatg acagattatt tcaggaagat ggaagttgcc ggaaatattt cggaaataat
    16021 gagtgtctcc gtctttacca atgtagtcat ctatctgctt ttcaaccgcc ttgacatgct
    16081 gagagcctgc aggggagtgc ttggaataac cctggcgtgg gcctttgttg tcttcgctat
    16141 aaacctgttt taggatgaag tacttcatca ttgcaggcga gccatccggt gatcttcacg
    16201 gttccaacct ggtgaagcac ctgctgaaga gtgatccggc agcagatatt gcatgctggg
    16261 gaggtgagct gatgcagggc gaaggtgcac ggctgctgaa acactaccgt gagactgcct
    16321 tcatgggcgt tttcgaggtg cttgtcaaca tccggaaggt gaaagccaac ttcagcgaat
    16381 gcaaaagaca gataatggag atgaaacccg atgtggtgat ccttatagac tatccgggat
    16441 tcaatctgcg tatggccagg tttgcaaagg aggccggcct cagggtcttc tactacattt
    16501 cgccgaaatt ctgggcatgg cgtgaaggaa gagtaagaaa gataaaacgc tatacagaca
    16561 ggctttacat catcttcccg tttgaaaagg agttttaccg gaagcatgat ttcccggcta
    16621 tcttccttgg caatcccctc attgaccata tagatgcatg gagggccaca gcgcctgaca
    16681 gggagatgat cagagcctca ctgggacttg acgccagacc ggtgattgca cttctctccg
    16741 gcagcaggct tcaggaggtg agcaaaattc ttccagtcat ggcagaagtc tctgaagact
    16801 ttcctgatta ccagttcgtg gtgacggcaa tggatcatct gcctgcccgg ctttatgaag
    16861 aggtctcttc agcataccct gtcagggtta tcaccggcag gacctatgac ctcctggctg
    16921 tggctgaggc cgcgcttgtc acctccggta ctgcaaccct tgaggctgcg ctctttgatg
    16981 tgcctcaggc ggtatgctac agtaccagca ggcttacata caggctggca aaatggctta
    17041 tcagggtcag gttcatctca ctggtgaacc tcatcatgga cagggaagtg gtgagagagc
    17101 tgattcagga ggggctgaat agtgccagcc tccacaggga actggaggag atagttgcca
    17161 ccggttacag acatgagagg ataaaggatg attaccggga gttgcggacc atactcggag
    17221 gtggcggggc atcagcgagg gtggcagcgg atatgataga aagtctggtt aacaagaaag
    17281 caggatgagg ataatgaaac ccatgatgga tgaagatatg gcagacactc ctgttcaaat
    17341 gaaaggaaaa tgaagagaat ctcactggtt gccatactgc tcgttctgtt tccgctggtc
    17401 acattctccc gtatctcaat acgtatcttt gcccggtcaa agccttccag cctgattgtc
    17461 agcatggtaa atggcgactg tatgcttttc gacggaacgg ccggagaggt gcgtctcagc
    17521 cagggcgaaa ccgttgccgt gacacggtat ggtgacaggg taatctacag cacactgtca
    17581 ggaataagag gggtatctga ttcactcgcc tttaccccgg ctgcatcagg cacgctcttc
    17641 actgtcaggg cacccggcag cagcgagcct gagaagagtc tcggcggcac gctgacagtc
    17701 acttcatttc ccggttcact ccggatactt aatatcacaa ccgttgaaga gtgccttccc
    17761 ggagtggtac gtgccgaggc gggcagatac ggaccgtcag attactacag ggcacaggcc
    17821 gtggtagcgc gaacctatat ctacaggaat attgacaggc attcacttga cgggtacaat
    17881 atgtgcgatg atgttcattg ccaggtctat ccgggagtgg tgaccgacag cgtgattgtc
    17941 aacgcctgcc gctcaacatc cgggctggtg cttgctgacc gcgacagcat aatgatagaa
    18001 gccgcgttcc acggtaactg cggcggcgag acggcatcct cagccgacgt gtggcttgcc
    18061 ggttatccct atctcgtgag cgtgacggac ccctggtgca gctattcccc atcatcaaca
    18121 tggaggaaaa acatagcgcg aagcgaatgg aacagtttcc tgagatcgaa gggtatagcc
    18181 ccgggaacgg agggggccat ctattcggcg aacgcaccgg cggcgggaag gataacatac
    18241 cgcaccgtac agggaaagac tctgtggagc gaagagatac ggctccgctt cgggctcagg
    18301 tcagcttact ttactctttc agacgccggt gacagcattc ttttcaacgg gagaggatac
    18361 ggtcatggtg tgggcctgtg ccaggacgga gcaaagcata tggccgaaaa aaagatgagt
    18421 tataacagga taatcggctt ctattacccg ggcaccatca ttaccgatgt caaaaatgcc
    18481 cgcaggccgg tcagaccatg aaagttttgg atatttttac agcaaa
//
LOCUS       DAOPBK010000029        18327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3144_length_18327_cov_5.374945, whole genome shotgun sequence.
ACCESSION   DAOPBK010000029 DAOPBK010000000
VERSION     DAOPBK010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18327
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3144_length_18327_cov_5.374945"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="PLA17_02955"
     CDS             complement(<1..2002)
                     /locus_tag="PLA17_02955"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPK84400.1"
                     /translation="MTDIPSKYDPASTEGKWYSYWLEKGLFRSRPDNRKPYTIIIPPP
                     NVTGVLHMGHILNNTTQDVLVRRARMLGFNACWVPGTDHASIATEAKVVAKLQSEGIE
                     KRSLSREQFIDHAWEWTHRHGGIILEQLKRLGASCDWDRTLFTMDEPRYKSVIKVFVD
                     LYEKGLIYRGVRMVNWDPQALTAVSDEEVIHTEEKSKLYYVRYKIVGEDNYIMVATTR
                     PETILGDTAVCANPNDERYAGMKGKKVIVPMANREVPFIFDEYVDMEFGTGCLKITPA
                     HDINDYEIGLKYNLESIDIFNDNGTISEAAGMFTGKDRFEARVLAEEELRRTGALVKV
                     EDYMNKIGRSERTHAVIEPKLSLQWFLKMKDVSKPALDAVMDDTVRLYPDKFRNVYRH
                     WMENVHDWCISRQLWWGHRIPAWYYNSNEFVVAVNEDEALEKARRVTGRADLTLNDLH
                     QDEDVLDTWFSSWLWPITCFDGINHPDNEDIKYYYPSNVLVTAPEILFFWVARMIIAG
                     YEYRGREPFGDVYLTGLVRDKQKRKMSKSLGNSPDPIELMEKYGADGVRVGMLLCSPA
                     GNDLLYDDSLPEQGRNFANKIWNAFRLVMKMSTDEMLEQPAASKAAVAWMRNTIDKTL
                     ADIEDSFVRYRISDALMQAYKLFWDDFSGWYLEIIKPAFGKPI"
     gene            2208..3404
                     /locus_tag="PLA17_02960"
     CDS             2208..3404
                     /locus_tag="PLA17_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPK84401.1"
                     /translation="MPKISEKAGMMPASPIRKLVPYAEAAKRRGVKVYHLNIGQPDIH
                     TPEIALNAVKNMDLKVIEYTHSAGNESYRRKLAESYRKIGIDIDHTEIIVTTGGSEAV
                     LFAFMTCLNPGEEVITFEPFYANYNGFAITAGVTIVPVRSFIKDDFALPSIDEIEKKI
                     TPKTKAILICNPNNPTGALYSKEELMKLGEIVKKHKLFLFSDEVYREFCYGAEHFSVM
                     QLEGIRDNAVLLDSVSKRYSMCGVRIGALITHNKEILAAALKFAQARLCPPELGQLAA
                     EAALDTPKEYFREVYDEYIARRDFMVNALNQMPGVYCPLPRGAFYTVVKLPIDDADRF
                     AQWLLEDFNYKNQTVMVAPATGFYFTPGAGKNELRVAYVLKIDDLRNAMETLAEALKV
                     YPGRVD"
     gene            complement(3516..4901)
                     /gene="gatD"
                     /locus_tag="PLA17_02965"
     CDS             complement(3516..4901)
                     /gene="gatD"
                     /locus_tag="PLA17_02965"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HPK84402.1"
                     /translation="MTEDFFQGYKGRALEVLKKFNVRVWGQADIETDRGLFKGTVLPR
                     SENNDDLHIVIKIESGYNIGIDIATIISMTETGYNKANYKIPEKEFPYSDDKPNVKLF
                     GTGGTIASRLDYRTGAVIPAFSPGELYGAVPELADICNIRTEKLFAIFSENMGPGQYK
                     LLARAIGDEIAKGIDGIMIGHGTDTLHHTAAALSFMVQDSPVPIVLVGSQRSSDRPSS
                     DAALNLIHASTAAGHGDIAEVMVCMFGPTSDEYGFLHRGTRVRKMHSSYRSTFRTIGD
                     IPVATVRRGEVKPIQKVYNPRRNDRNVKIMPYFNENVTMVYFYPHMKADMIHSLIDNG
                     YKGIVIVGTGLGHVNRELYPALERARRENVAVYMTLQTIWGYVHMFVYETGRDMMAKG
                     VIPLGNMLPEVAWVKLGWALGQTDDLEEVKRIMLTPINDEITEREPYNGYLVYQGGVP
                     EVEEFIRRVHK"
     gene            complement(4907..6889)
                     /gene="gatE"
                     /locus_tag="PLA17_02970"
     CDS             complement(4907..6889)
                     /gene="gatE"
                     /locus_tag="PLA17_02970"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HPK84403.1"
                     /translation="MSIIDPRKNYEETREEIGYVARKKATLNDYKRIGFKSGLEVHQQ
                     LLTRNKLFCRCPAGIYHDDNDFDAEVIRHMRPTLSEMGVYDGTALMEFRTRKEITYRI
                     NNETACTYEIDDTPPFPVDREALEIAISIALAARMNIVGEVHITRKQYLDGSIPAGFQ
                     RSAIISMNGELQLKSRKVRLIQLSLEEDACREVSDIGHKRIFKTDRLGMPLIEIVTCP
                     DCLTPDELAEAAEYIRFLNRSTGKVRTGMGAAREDVNVSCEGGNRVEIKGVAHIRWIP
                     ELSHNEAFRQYSLLNIRSKLLKRIKDPAAWSVKTKEITERDQEFHFAPVRSAIDRGER
                     VIAMNLPGCRGILSHFTQPGKIFADEISDRLKVIACIEKPNMTHSEEISGGNFNNLFP
                     ELAIWLSADAGDAQIIIWGPDEDIPTAIETVEERIRLAFGGVPNETRKAFKDGTTMFE
                     RVLPGRDRMYPDTDSQPIAVTTEMLEKLSRNIPVEISERFIQMKEWNLPEDTWHYLLS
                     KNLVVLIERISNDFGFDPKEIGILTGHRFRSLEGRGRVAAGFSYERLYDLFAFLKNEK
                     LLTPVARIMLPAAVAVASPDFRSILAGTGLRRATMNEIREEAKELSARFPEICHNRNM
                     AGMEEWIMGQLHLKALGTVKLSEVRNMVTELLEAGD"
     gene            complement(7119..8363)
                     /locus_tag="PLA17_02975"
     CDS             complement(7119..8363)
                     /locus_tag="PLA17_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK84404.1"
                     /translation="MSIGTFIASRLIKDRNRGTAFSRPINVIAIIGIALGLAVMILAV
                     SILTGFKQQIRDKVAGFGAHIQVVNFDANLSYETVPISSNQPFVPKIREHRGIRNVQV
                     FATKAGIIKTDENIQGVVLKGIGSDYDWSFFRSNMVEGETLSVSDTARSNDVVISKKV
                     ADMLGFMPGDSFSMFFVQDPPRMRKFTVLGIYETSLEEFDKTYLFCDINHIRSLNGWS
                     DDQISGFEIYINDFDQLDYMTDVIRDSIGYRISEEEEQLKVTNIRGRYPQIFDWLGFQ
                     DTNVIIILVLMILVAGFNMISGLLILILEKTNMIGVLKALGAGNKVIRRIFLYQAAWL
                     TAKGLFWGNIIGIGLALLQLRTEMISLDPSSYYLSSVPINLDPVHIILLNIGTMIAII
                     LMLLIPSQLIARITPVKAIRYN"
     gene            8441..9616
                     /locus_tag="PLA17_02980"
     CDS             8441..9616
                     /locus_tag="PLA17_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPK84405.1"
                     /translation="MKKLITPLLIVAMVITACSAGNENTTLMTGAERTVEYLPLLRGK
                     RVAVVANQTSMVSKTHLVDTLLSEGVDIRLIFAPEHGFRDLADDGTVITSGSDPVTGI
                     AVISLYSSKKKPVPEDLADIDVVLFDLQDVGTRFYTYLTTMCYVMEACAENGKQFMVF
                     DRPNPNGYYVDGPILDTASYRSFVGIHPIPVVHGMTFGEYAGMVNGEGWLAGGIRCDL
                     KVISCTGYTHATMYRLPVIPSPNLPNMNSVYLYPSLCFSEGTVLSCGRGTGFPFQVLG
                     APEIPDTGFSFTPQPSFGSSNPKHNGVTCYGIDLRNATADGLVPKPEINLDWIIEMYR
                     AYPDKENFFNGYFDTLAGSSTLREQIISGMSATEIRASWKEGLERFSATREKYLLYD"
     gene            complement(9642..11060)
                     /locus_tag="PLA17_02985"
     CDS             complement(9642..11060)
                     /locus_tag="PLA17_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPK84406.1"
                     /translation="MRKLISFLFIAFLSVISCEKGNRRVYTERVLPRDLDAILAEGKI
                     RAVTNINQTSYFIYNGEPMGFHFELLKKFADHLELELEIITSNDIDEALEYLRSGEAD
                     LVAIGLSINADRKEMMQFTEPLIQSSQLLVQRKPNGWEKMTEEAVAEKLVRNQLDLAG
                     KTIYVQKGSSYAQRLYNLERESGIDIGIIEVPYDAEELARQVGRGEIEYTICDDYMSN
                     IIATLYPVLDMSTPVSFPLNIAWSVRKEGTGALVEKLNGWITGFKTTREYARLERKYF
                     SGLQPASMALSEYFATNSYGKVSPYDDIIKNYADTIGWDWRLVAALIYQESRFNPSVT
                     SPRGAYGLMQVMPSTGRHFGFDVKRSVENNIRAGVSYIRMLDRLFSQWVSDPDERVKF
                     ILASYNAGHGHVIDAIRLAEKNGLDPGRWDDNVSLFLERKADPAFYADPVVRNGRLKQ
                     GVQVNAYVADILKRYKHYRNIK"
     gene            complement(11133..13247)
                     /locus_tag="PLA17_02990"
     CDS             complement(11133..13247)
                     /locus_tag="PLA17_02990"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HPK84407.1"
                     /translation="MRRVPLYIASFLLFLPLTLAGQEPDTLRAVVRSDTATVTRSDTT
                     AVVQQDTTTVTRSDTTRSHKERGLPGALPAEAGARPGIPDAETGAEIDTTFTTASRQW
                     TLSSDFTTEIPVVLDTAFSLFHRYRITDSYSDFNAYTGNYGLPLYQINFFDREWKPDR
                     YLYIYYLPFMFTPLNTLFINTRVPFTELKWSNGGARSVAEQTFRIRHSQNVNRKLNIG
                     LIYDIVYNIGQYKLQKAIDKNFLLHGSYNSNDYNVYASAGINNHESGESGGMISKDDL
                     KQYNPEGVPFRLSDLSSAKSTLKNRYLMLVQRYAPGGRRDTVTGEVLKSGPITFSHIG
                     ILEWSKRGYIDKYPAADLYDTIMINRSVTADSLQERILSNTLRVDFAAGRTDRFRIGA
                     GAGIRSELRSYGQIMPGDTLTRPDTIRRKMNSLVLTGKVFNNIGSKFGWSASGDLWFQ
                     GYRAGDFIVDGRIYKEFETRRRGMITWDATGTIASYTPSFWYYSWGSNNFAWQLDHKR
                     EFRLIAGSSLKWPDLKFSLKFNYAIVDNFIGMGTGSLPYQHEGGLSLLALTAKKEFVI
                     WKLHLDNTLLLQQSTNTDVLDLPLVTARSAFFFDHVFRFKSTGGELSLQLGAEAMIHT
                     LYDAMSYMPATGRYFNQNDGKTGNYPFVNAFINLKIKRTRIFVMADHLNSGLTGYEYF
                     LVPGYPLNIRMIKYGLAWTFYD"
     gene            complement(13464..14948)
                     /gene="ftsZ"
                     /locus_tag="PLA17_02995"
     CDS             complement(13464..14948)
                     /gene="ftsZ"
                     /locus_tag="PLA17_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPK84408.1"
                     /translation="MSDDLMNFDLPVDRSSIIKVLGIGGGGGNAVNHMYRKGIKDVNF
                     IVCNTDLQALMNSPVPVRVQLGETTTEGLGAGSQPERGRASAIENMEDVMKALSGNTK
                     MVFITAGMGGGTGTGATPVIAQACKDAGFLTVAVVTIPFRSELNQRIKLAISGINELR
                     DKVDSLIVINNERLRQIYGDEGVSAAFARADDVLANAVKGIAEIITCTGYINVDFADV
                     DTVMRDSGVALMGIGNASGEERATRAIEAALTSPLLSSSDITGARSLLLNIKSGTGAN
                     EITMDELGELTDYVYETVSDDALIIRGLSTDEELGDAISVTVVATGFEECADLEGVKP
                     NLRKEVVSLNSAPPQPGHADAASENDGQYIVRSTRASDRAIPPHSASFNQGVIDFEPD
                     GDIDISPAPARPAQQAPDRNVGTLRKLKHMQNVIRNEGLSTQSMSDNIEDFEEVPAYV
                     RKKITIHNASKAAGSKVSKFTLTLDEDNQPVIRENNAYLNDNVD"
     gene            complement(14958..16352)
                     /gene="ftsA"
                     /locus_tag="PLA17_03000"
     CDS             complement(14958..16352)
                     /gene="ftsA"
                     /locus_tag="PLA17_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPK84409.1"
                     /translation="MSNKDQLVAAIDIGTTKIVAIVGRSDGNGGIEILGLSNTPSTGV
                     KRGVVLNIEETVRAIRTTVDDLEERTGLVLTDVFVGIAGRHIKSKMARGYITRDSHET
                     EIDINDIRRLTADMYKLPIDVGEEIIHVIPQTYIVDNESGINNPIGICGKRLEANFHI
                     VIGQIASARNTERCINKAGMNLMSLMLEPLASSEAVLTDEEKEAGVVLVDIGGGTTDV
                     AIYYDNVIRHTAVIPFGGNMVTADIREGCSILQKQAEDLKIKFGFAIGDIAPENKVVS
                     IPGIAGREPKEISFKNLAYIIQSRMEEILDAVNYEIQNSGFAEKLTAGVVVTGGGAML
                     KNLPQLVRFKTAMDVRIGHPNVHLTGKGRDEISQPMYATSVGLILQGIEYLEETRTFT
                     RIGVKNQEPVAEHQQPMHTPPPVKEEPAEVEHDELFHDGQRRTGKFLGDRLRGLWDKI
                     IEATDEKLDDTPTK"
     gene            complement(16452..17225)
                     /locus_tag="PLA17_03005"
     CDS             complement(16452..17225)
                     /locus_tag="PLA17_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HPK84410.1"
                     /translation="MSILMRIVTIVTGAALFLTPVFISKMTADAICEGVVINLADSSK
                     HQFVTNNDIMNALRATGIRVTGQKIRDIPLTKLEERVKAFTELRVAEVYISEDRKLHV
                     YGNQREPVMRVMASYGGDFFIDREGVIMRRHNLYTPNLHILEIDMVFNSEQMTGTNIF
                     ESEKTANLAQAFELVNYIRGDSFWNGMIDQLSMSRDGRVTLVPHVGNHTVKMGRVENY
                     EEKLHNLRVFYRQAMPVAGWDRYRVINVEYKGQVVCQRR"
     gene            complement(17319..>18327)
                     /locus_tag="PLA17_03010"
     CDS             complement(17319..>18327)
                     /locus_tag="PLA17_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPK84411.1"
                     /translation="TVSTMTAHWLSSSPVGCSAFLGGISVNYNTNLLLSESRYTVMEA
                     DEFDRSFLHLSPLIAVVTAIDPDHLDIYGTAESMVEAYEEFCHRVRPGGTLILNENIA
                     SSLSLPGDVNIYTYGTGAKASFRAESVRKEEGSYTFDIITPGESVRNVRIHLPGMVNI
                     LNATAASAAAWCAGVSPEIIRSAVGSYRGVRRRFDVRYSGNDIIYIDDYAHHPQEINA
                     LVSAVRDFWPGKHVTGIFQPHLYSRTRDFADGFAEALSRLDEIILLTLYPAREMPIPG
                     VSSDMIAGMMKNRNVKVVTKEELLPALNSVKKGLLLTIGAGDIDRFVEPITEMLKNRN
                     A"
BASE COUNT         4284 a         4946 c         4439 g         4658 t
ORIGIN      
        1 caatgggctt gccgaaggcg ggcttgatga tctcaagata ccagccggag aaatcatccc
       61 agaagagttt gtaggcctgc atcagcgcat cggagatgcg gtagcgcaca aagctgtcct
      121 ctatatccgc aagggtcttg tctatggtat tgcgcatcca agcgacagcc gccttcgatg
      181 ccgccggctg ttcaagcatc tcatcagtac tcattttcat cacaagccgg aaggcattcc
      241 atatcttgtt ggcgaagttg cgcccctgct ccggaaggct gtcatcatac agaaggtcat
      301 tccccgcagg ggaacagagg agcataccca cccttacacc atcagcaccg tatttctcca
      361 taagctctat cgggtcagga gagttgccga gtgatttcga catcttgcgc ttctgcttgt
      421 cgcgcaccag ccctgtaagg tatacatcgc cgaatggctc acggccccgg tattcatatc
      481 cggcaatgat catcctggca acccagaaaa agagaatctc cggagcggta accaggacat
      541 ttgaggggta gtaatattta atgtcctcgt tatcagggtg gttaatgccg tcaaagcagg
      601 ttatgggcca tagccatgaa gagaaccacg tatcaagcac atcttcatcc tggtgaaggt
      661 cattcagggt caggtctgcc ctgccggtga cgcggcgggc cttttccagg gcctcatcct
      721 cattcacggc aacaacaaac tcattgctgt tataatacca tgccggaatc cggtgacccc
      781 accacagttg cctgctgatg caccagtcat gcacattctc catccagtgc ctgtaaacat
      841 tcctgaactt gtcagggtag agcctgacag tatcatccat caccgcatca agggcaggct
      901 tggagacatc cttcatcttc aggaaccact gcagtgagag ctttggttca ataaccgcat
      961 gagtgcgctc tgacctgcct attttattca tgtaatcctc aacttttaca agagcgcctg
     1021 tacggcgaag ttcctcttca gcaaggacgc gggcttcgaa acggtctttg cccgtgaaca
     1081 tgcctgccgc ttccgagatg gtaccgttat cattgaaaat gtctatactc tccaggttat
     1141 acttcaggcc gatctcatag tcgtttatgt catgagccgg ggtgatcttc aggcaccctg
     1201 tcccgaactc catgtcaaca tactcatcga agatgaaagg aacctcgcgg ttggccatgg
     1261 gtacaatgac cttcttcccc ttcatgcctg cataacgttc atcattcggg ttggcgcata
     1321 cagccgtatc accaaggatg gtctccggcc tggtggttgc caccatgata taattgtcct
     1381 caccaactat tttgtaacgc acataataga gtttcgactt ctcttcagtg tggataacct
     1441 cttcatcaga gacagccgtg agcgcctgcg gatcccagtt gaccattcgt actccgcggt
     1501 agatcaaccc cttttcataa agatcaacaa agacctttat cactgatttg taacggggtt
     1561 catccatggt gaacagggtg cggtcccagt cacatgatgc cccgagtctc ttcagctgct
     1621 caaggatgat gcctccgtgc ctgtgtgtcc actcccaggc gtgatcaata aactgctcac
     1681 gtgaaagtga tctcttctct attccctcac tttgcagctt ggcaacaacc ttggcctcgg
     1741 tagctatgct ggcatgatcg gtgcccggca cccagcaggc gttgaaacca agcatacgcg
     1801 cacgcctgac aagcacatcc tgggttgtat tgttgagtat gtgccccatg tgaagcaccc
     1861 cggttacatt cggaggagga atgataatgg tgtaaggttt gcggttgtca ggcctgctgc
     1921 ggaaaagccc cttttccaac cagtaactgt accatttccc ctctgtggaa gccggatcat
     1981 attttgaagg aatatcagtc atgggatttg atatttgata tttgttcgat gtaatgtggc
     2041 aaacttctgt ttattggctg gtaattcaat gtttaagggc acaaatttaa caatttgcag
     2101 ccattatgta aaaagaggcc gggcttatgc gtaaaatcat tttcatgtta tctgaatttg
     2161 aatacatttg gaacgctaag ttatcagtgc tttataaatt tacagtcatg ccaaagattt
     2221 cagaaaaggc cgggatgatg ccggcatcac ccatccgcaa gcttgtacca tacgccgagg
     2281 cagcaaaacg caggggagta aaggtctacc acctcaacat agggcagcct gacatacaca
     2341 ccccggagat tgctctcaat gctgtgaaga atatggacct gaaggtcatt gagtacaccc
     2401 attccgccgg gaacgagtca taccgccgta aactggctga aagttacagg aagatcggca
     2461 ttgacattga ccatactgag atcattgtta ccacgggcgg ctccgaggct gtcctcttcg
     2521 cattcatgac atgcctcaac ccgggcgaag aggtgatcac cttcgaacct ttctatgcca
     2581 actacaacgg tttcgccatc acggccggcg tcacgattgt accggtcagg tcattcatta
     2641 aggacgattt tgcactgcct tcaattgatg agattgagaa gaagataacc ccaaagacga
     2701 aggcaatact gatttgcaac ccgaacaacc ccaccggcgc tctctattcg aaggaagagc
     2761 tgatgaaact gggcgaaata gtgaagaaac ataaactttt cctcttctct gacgaagttt
     2821 acagggagtt ctgttacggt gcagagcact tctcggtaat gcagctggaa ggtataagag
     2881 acaacgctgt gctgcttgac tcagtttcaa agagatacag catgtgcgga gtgcgtatcg
     2941 gagctctgat cactcacaat aaggagatcc ttgctgcagc actcaagttt gcccaggcaa
     3001 gactctgccc gcccgagctg ggtcagctgg cggctgaggc tgcccttgac acgcccaaag
     3061 agtatttcag ggaggtttac gatgagtata ttgcccgccg tgatttcatg gtgaatgcac
     3121 taaaccagat gccgggtgtc tactgtccgc ttccccgcgg tgcattctac acggtggtga
     3181 agctgccgat agatgacgcg gaccgttttg cacagtggct gctcgaagat ttcaactaca
     3241 agaatcagac agtcatggta gcccccgcaa caggatttta cttcaccccc ggcgccggca
     3301 agaacgagtt gcgtgttgca tacgtcctga agattgatga cctgcgcaac gccatggaaa
     3361 cgctggccga ggcgctgaag gtgtaccccg gaagggtcga ttgattattt gaagcctgca
     3421 cccggcaggc acttccgggt caacatgatg tcatattact ctttcaacgc agaaaaccat
     3481 tgcctgctgc ctggcggcac tctcttctgc ctgctctact tgtgtacccg cctgatgaat
     3541 tcctccacct ccggaacccc gccctggtac accaggtaac cgttatacgg ctcccgttca
     3601 gtgatctcgt cattgatggg ggtgagcatg atccgcttca cctcctccag gtcatcggtc
     3661 tgacccagcg cccagcccag cttcacccat gcaacctcgg gcagcatgtt gcccagcggt
     3721 atcacaccct tcgccatcat gtcacgaccg gtctcatata caaacatgtg aacataaccc
     3781 cagatagtct gcagcgtcat gtataccgca acattctccc gccgggctct ctccagagcc
     3841 gggtagagtt ctctgttcac atgtccgagc ccggttccca ctatgacaat ccccttgtaa
     3901 ccattgtcaa tgagtgaatg gatcatatcc gccttcatat gcggatagaa ataaaccatg
     3961 gtcacattct cattgaaata tggcatgatc ttcacattgc ggtcattgcg ccgcgggtta
     4021 tataccttct gaatgggctt cacctcaccc ctccttaccg ttgccactgg tatgtcacct
     4081 attgtccgga aagtggacct gtatgagctg tgcattttcc gtacccttgt gccgcggtgc
     4141 aggaaaccgt actcatcact tgtaggaccg aacatgcaga ccatcacttc cgcgatgtca
     4201 ccatgcccgg cagccgtgga ggcatgtatc aggttcaggg cagcgtctga cgacggccgg
     4261 tcagatgacc tctgcgatcc gacgagcact atcggcacgg gagagtcctg taccatgaac
     4321 gagagcgctg cagccgtatg atgcagtgta tcagttccat gaccgatcat gatgccgtca
     4381 attccttttg cgatctcgtc gcctatggcc ctggcaagca acttgtactg gccggggccc
     4441 atgttctcgc tgaatatggc aaagagcttc tccgtgcgta tattgcatat atcggccagc
     4501 tcgggcacgg caccgtacag ctcaccggga gaaaacgccg gaattactgc accggtgcgg
     4561 taatcaagcc tcgaggcaat ggttcccccc gtaccaaaca atttgacatt gggtttgtca
     4621 tcagaatagg gaaattcctt ctcggggatc ttataattgg ccttgttgta accggtctcc
     4681 gtcatgctga ttatggtggc aatatcaatg ccgatattat accctgactc tatcttgatc
     4741 acgatgtgga gatcgtcatt attctctgaa cgcggcagga cggtcccttt aaacagcccc
     4801 ctgtcagtct ctatatcagc ctgaccccag acacggacat tgaacttctt cagcacctca
     4861 agcgccctgc ctttgtatcc ctggaaaaaa tcttcagtca taacactcaa tctccggcct
     4921 caagtaattc tgtcaccata tttctcactt ccgacaattt cacggttccg agcgctttca
     4981 aatgcaattg tcccattatc cactcttcca tcccggccat attgcggtta tggcatattt
     5041 ccgggaaccg tgcggagagt tcttttgcct cctccctgat ttcattcatc gtggcacgtc
     5101 tgagcccggt tcctgccagt atactcctga aatcaggaga tgccactgca actgctgcag
     5161 gaagcattat tcgcgccacc ggcgtgagaa gcttctcatt cttaaggaag gcaaagaggt
     5221 catatagtct ttcatagctg aatcctgccg ccaccctgcc ccgcccttcg agactgcgga
     5281 aacggtggcc tgtcagtatg ccgatctcct tcggatcaaa gccgaaatca ttgctgatcc
     5341 gctcaatcag taccaccagg ttcttgctca gcaggtaatg ccaggtgtct tcgggaaggt
     5401 tccactcctt catctggatg aaccgttctg aaatttcaac cggtatgttt ctgctgagct
     5461 tctccagcat ctccgtcgtg acagctatgg gctgtgaatc ggtgtcagga tacatgcggt
     5521 cacgaccggg aagcaccctc tcaaacattg ttgtgccatc tttaaacgcc ttgcgggttt
     5581 catttggcac tcccccgaag gccaggcgta tccgctcctc aactgtttca atggcagtgg
     5641 ggatatcttc gtcagggccc catattatta tctgggcatc accggcatcg gccgaaagcc
     5701 atattgccag ctccgggaag aggttgttga agttgccccc ggagatttcc tccgaatggg
     5761 tcatgttggg cttctcgatg caggctatca ccttgagcct gtcactgatc tcatctgcga
     5821 aaatttttcc gggctgggta aaatgcgaga gtatacccct gcagccgggc aggttcatgg
     5881 caatgactct ctctccgcgg tcgatggccg acctgaccgg tgcgaaatga aactcctgat
     5941 ccctttctgt gatctccttt gttttgactg accatgcagc cgggtccttt attctcttca
     6001 gcagcttcga gcggatgtta agcagcgagt actgtctgaa tgcttcgttg tgtgacagct
     6061 ccggaatcca tctgatatgt gccaccccct tgatctccac ccggtttccg ccctcgcagc
     6121 tgacgttcac gtcttcgcgc gcagcgccca ttcccgttct caccttgcct gtactgcggt
     6181 taaggaacct gatatattcg gccgcctcgg ccagctcatc aggagtgagg cagtcaggac
     6241 aggtcacgat ctctatcagc ggcatgccca ggcggtcagt cttgaatatc cgtttgtggc
     6301 ctatgtctga cacttcgcgg cacgcgtcct cctccaggct gagctggatg agcctcacct
     6361 tcctgctttt cagctgaagc tctccattca tgctgattat ggcggagcgc tggaatccgg
     6421 cgggaatgct tccgtcaaga tactgtttac gggtgatatg cacctcaccc acgatgttca
     6481 tcctggcagc cagtgcaatg cttatggcaa tctccagcgc ctcacggtca acggggaaag
     6541 gaggggtgtc atctatctcg tatgtgcagg ctgtttcatt gttaatacgg taggtgatct
     6601 ccttcctggt tctgaactcc atgagcgcag tgccgtcata gactcccatc tcactgagag
     6661 ttggtctcat gtgacgaatc acctccgcgt caaaatcatt gtcatcatgg taaataccgg
     6721 ccggacaacg acaaaagagc ttgtttctgg tcagcagttg ctggtgcacc tcgaggcccg
     6781 acttaaaacc aatccgctta taatcattaa gcgtcgcctt tttacgggca acatacccga
     6841 tctcctcacg ggtctcttcg tagttcttcc gcggatcaat tattgacata cctgaagaca
     6901 ttgcatctgc aatttacgca gaagcccgga atattggaag tgtccattta aagaaagttt
     6961 gcggaacttt ctgctattaa atgttccaat gcctggcgat taaaattatg ttgaaatcaa
     7021 gagaagggga tttataaaat aaatcttgtg gaagaatatt ggtatttgca ttaaaatgga
     7081 aggagcttaa taatattaag cgcgcagtaa cctgattatc agttgtatcg gattgctttg
     7141 acaggtgtaa tgcgggcaat gagctgagaa ggtataagaa gcatcaggat aatggcaatc
     7201 atcgtcccga tgtttagcag gattatatga acggggtcaa gattgatcgg aacactgctg
     7261 agataatatg aggagggatc gagcgagatc atttctgtcc tgagctgcag cagcgccagc
     7321 ccgataccta ttatgttacc ccagaaaagg cccttggccg tcagccaggc ggcctggtaa
     7381 agaaaaatcc tgcggataac cttgttgccc gctccgagag ccttcagcac acctatcatg
     7441 ttggtcttct caagtatcag aatgagcagg ccggaaatca tattgaaccc ggcgacaagg
     7501 atcataagca ccaggatgat tatcacgttg gtgtcctgaa atcccagcca gtcaaatatc
     7561 tgcgggtaac gccccctgat gttggttacc ttgagctgct cctcttcctc actgatccgg
     7621 tagcctattg aatcacggat aacatcagtc atgtaatcaa gctggtcgaa atcatttatg
     7681 taaatttcaa agccgctgat ctggtcatca ctccagccgt tgaggcttct tatgtggttg
     7741 atgtcgcaga agaggtaggt cttgtcaaac tcctcgaggc tggtttcata gattcccaga
     7801 acggtgaact tgcgcatccg gggcggatcc tgaacgaaaa acatcgaaaa tgagtccccc
     7861 ggcatgaaac ccagcatgtc agccaccttc ttcgagatga ccacatcatt gcttcgtgcc
     7921 gtgtcactga cacttaacgt ctctccctcc accatgttcg acctgaagaa actccagtca
     7981 tagtcactgc caatcccctt gagaacaact ccctgaatgt tttcatcagt cttgatgatt
     8041 cccgccttgg tggcaaagac ctgcacgttc cttattccac ggtgctccct gattttcggg
     8101 acaaagggct ggtttgagct gatgggcaca gtctcgtagg aaagattggc atcaaagtta
     8161 accacctgga tatgagcccc gaagcccgcc accttgtcgc gtatctgctg cttgaagccg
     8221 gtgaggattg acactgcgag gatcatcact gccagcccca gggcgatgcc gataatggct
     8281 atgacattta ttggccgtga aaaggcagtg ccacggttgc ggtcctttat caggcgactg
     8341 gctatgaatg ttccgattga cattgcgaca aaattaagaa ttaagcgcga agaatctgtt
     8401 tattgcccct tttcttatct ttacacgaaa cctgactgaa atgaaaaaac taatcactcc
     8461 gctgctcatt gtagcaatgg tcattacggc ctgttcggcc ggaaacgaaa acaccaccct
     8521 gatgacaggc gccgaaagaa ccgtcgagta cctgccgctg ctcaggggaa aaagagtggc
     8581 ggtggtggcc aatcagacct cgatggtcag caagacccac ctggttgaca cactgctctc
     8641 cgaaggtgtc gacatccgtc tgatctttgc tccggaacat ggtttcagag accttgccga
     8701 cgatggtaca gtgatcacct cgggcagtga tccggtaacc ggcatagcgg tcatcagcct
     8761 ctattcctcg aagaaaaaac cggtgccgga agatctcgct gacatagatg ttgttctctt
     8821 tgacctgcag gatgtgggaa ccaggtttta cacatatctg accaccatgt gttacgttat
     8881 ggaggcatgc gccgaaaacg gcaagcagtt tatggtgttt gacaggccga acccgaacgg
     8941 ttattatgtt gacggaccaa tacttgacac cgccagttac cgctctttcg tgggaattca
     9001 tcccataccg gtggtgcatg gaatgacctt cggcgagtat gccggcatgg tgaacggtga
     9061 ggggtggctg gccggaggca tcaggtgcga cctgaaggtg atcagctgta ccggctacac
     9121 ccatgccacg atgtaccggc tgccggtgat accgtcacct aaccttccca acatgaactc
     9181 tgtctatctc tacccgtcac tctgcttttc ggaagggact gttctgtcat gcgggagggg
     9241 taccggtttt cctttccagg tgcttggagc tccggagata ccggatacgg gattcagctt
     9301 caccccgcag ccttcattcg ggtcttccaa tcccaaacat aacggagtga cctgctatgg
     9361 catcgacctt cgcaatgcga cagctgacgg cctggtgccg aaacctgaga taaaccttga
     9421 ctggataatt gagatgtaca gggcataccc tgacaaggag aacttcttca acggttattt
     9481 tgacaccctg gccggtagca gcacgctccg cgagcagatc atcagcggca tgagtgccac
     9541 ggaaataagg gcgtcatgga aggaggggct ggagaggttc agcgccacga gggaaaaata
     9601 cctgctgtat gattagcaac catgttttgt tcagtgtatc gctacttgat gttccggtaa
     9661 tgcttgtacc gcttcaggat atccgccaca taagcgttga cctgcacacc ctgcttcagc
     9721 ctgccgtttc tcaccaccgg atcggcataa aacgccggat cagccttcct ctccaggaaa
     9781 agtgacacgt tgtcatccca cctgcccgga tcaagcccgt tcttctcggc aagcctgatt
     9841 gcatcaatca catgtccatg accggcattg taactggcaa gaatgaactt caccctctca
     9901 tcgggatcac tcacccactg gctgaataac ctgtcgagca tcctgatata gctgacgccg
     9961 gcgcggatgt tgttttcaac cgatctcttc acgtcaaacc cgaagtgccg cccggtggaa
    10021 ggcatcacct gcatcaggcc gtaggccccg cggggcgaag tgaccgacgg gttaaacctc
    10081 gactcctggt agatcagggc tgcaacaaga cgccagtccc aaccaatggt gtcagcataa
    10141 ttcttgatga tgtcatcata cggtgacacc ttgccgtaag agttggtggc aaaatattcc
    10201 gaaagggcca tggatgcggg ctgtaatcca ctgaaatact tcctctcaag cctggcatac
    10261 tcacgtgtgg ttttaaaccc ggtgatccac ccgttaagct tttcgacaag ggcgccggta
    10321 ccctctttcc tcacactcca cgcaatatta agagggaagc tcactggagt tgacatgtca
    10381 agaacgggat agagagtagc gatgatgttc gacatgtagt cgtcacatat tgtgtattct
    10441 atctctcccc tgcccacctg gcgggcgagc tcctctgcat catagggaac ctctatgatg
    10501 cctatgtcaa tacctgactc acgctcaaga ttatagagac gctgagcata tgatgatccc
    10561 ttctgtacat agattgtttt ccccgcaaga tcaagctggt ttcttaccag cttttccgcg
    10621 actgcctctt ctgtcatctt ttcccacccg ttgggtttgc gctgcaccaa cagctgactc
    10681 gactgaatca ggggctcggt aaactgcatc atctcctttc ggtctgcatt tatacttaga
    10741 ccgatagcca cgagatcagc ctcgcctgac cggagatact caagcgcttc atctatatca
    10801 ttgctcgtaa ttatctcaag ttcaagttcc aggtgatctg caaacttttt gagcaattcg
    10861 aaatgaaagc ccatcggctc tccattatag atgaaatagc tcgtctggtt tatattggtt
    10921 accgccctga tcttgccttc agcgagtatt gcgtcaaggt cccttggcag aactctctcc
    10981 gtataaaccc ttcggttccc cttttcacag gagataactg acaaaaaagc aataaacaag
    11041 aacgaaatta acttcctcaa tacctaaaca attagttcga caattcaatt ccctaaaaca
    11101 ggaattattg acggcgcaaa gataaaataa ttttaatcat agaaagtcca tgccagtccg
    11161 tattttatca ttctgatatt cagaggataa cccggaacaa ggaaatattc atacccggtc
    11221 aggcccgaat tcagatgatc agccatgacg aaaattctgg ttctttttat cttcagattg
    11281 ataaaggcgt ttacgaaagg ataattgccc gtttttccgt cattctggtt aaaatatctg
    11341 cccgttgcag gcatgtaact cattgcatca tagagagtat ggatcattgc ctctgccccc
    11401 agttgaaggc ttagttcccc tccggttgat ttaaacctga aaacatggtc gaaaaagaag
    11461 gcactgcgtg ccgtaactag cggaagatca agaacatcag tattagtgct ctgctgcagt
    11521 aagagggtat tgtcaaggtg cagcttccat attacaaatt ccttcttcgc ggtgagcgca
    11581 agaagagaga ggcctccctc atgctggtaa gggagagagc ctgttcccat acctataaag
    11641 ttatcaacaa tggcatagtt gaacttcagg ctaaatttta gatcgggcca cttcagagat
    11701 gagcctgcaa tgagcctgaa ctcacgttta tggtcaagct gccaggcgaa gttgttggag
    11761 ccccatgaat agtaccagaa tgacggggta taactggcaa ttgtgcctgt tgcatcccag
    11821 gtaatcatac cacgccggcg tgtctcaaac tccttgtata tgcggccgtc aacgatgaag
    11881 tcacctgccc ggtacccctg gaaccagaga tcaccggagg ccgaccatcc aaacttgctt
    11941 ccaatgttgt tgaaaacttt tcctgtcagg acaagggaat tcatctttct cctgatcgta
    12001 tcgggtctcg taagtgtatc tccgggcata atctggccgt atgagcggag ttcactcctt
    12061 atccctgcac cggctcctat cctgaaacgg tcggttcgcc ctgcggcgaa atccacacgc
    12121 agggtattgg agagaatacg ctcctgaagc gaatcggcag tgacactcct gtttatcatt
    12181 atggtgtcat acaggtcagc agcgggatat ttgtcaatgt aacctctctt gctccactca
    12241 agtatgccga tatgtgagaa agtgatggga ccgcttttca gtacctcgcc ggtgacagtg
    12301 tcacgcctgc ctcccggggc ataccgctgt accagcatca gatagcggtt cttaagggtg
    12361 ctcttcgcgc tgctgagatc actgagacgg aatggaacac cctcaggatt atactgctta
    12421 aggtcatcct ttgaaatcat gccgccgctc tcccctgact catggttatt gatgcccgcg
    12481 gaagcatata cattataatc atttgaattg taggagccat gcagcaggaa gttcttgtct
    12541 atggccttct gcagtttata ctggccgata ttgtaaacta tatcataaat gaggccgatg
    12601 ttgagtttcc tgttgacatt ctgcgagtgc ctgatcctga aagtctgctc ggccacgctc
    12661 ctcgccccgc cgtttgacca cttcagctcg gtgaaaggaa cacgggtatt gatgaagagg
    12721 gtgttcaacg gggtgaacat gaagggcagg taatagatat acagatacct gtcaggcttc
    12781 cattcacggt caaagaaatt gatctggtac aggggcaggc cgtagttgcc ggtgtaggca
    12841 ttgaaatccg agtaactgtc tgttatcctg tagcggtgaa acagcgagaa agcggtgtca
    12901 agcaccaccg gtatctcggt ggtgaagtcg gatgagaggg tccactggcg ggaggcggta
    12961 gtgaatgtgg tgtcaatctc cgcgccagtt tccgcgtcag gaatgccggg ccgggcgccg
    13021 gcctctgcag gaagtgcccc cggcagcccg cgctctttgt gagaacgggt ggtatcgctc
    13081 cgggtcaccg tagtggtgtc ttgctgcacc acagcagttg tgtcgctccg ggtcaccgtt
    13141 gcagtatcgc tcctgacgac agccctgagg gtgtcaggct cctgtcctgc cagcgtaagc
    13201 gggaggaaca gaaggaaaga agctatatac agcggcactc tgcgcatcac cggcaaattt
    13261 actgaaaaag ttgcattgca gttgagttga gcttgaggat gaagaaaaag gagacggctt
    13321 ttacaaaatt gttggagccg gatcagaaaa aagcaggggc cgggtcagaa aaagcaggag
    13381 ccgggtcaga aaaagcagga gccgggtcag aaaactctgc ccggctccgg tgaacaacaa
    13441 ccatataata gcaaaaaaca aaatcagtct acattgtcat tcagatatgc attattttcc
    13501 ctgatgacag gctggttatc ctcatcgagg gtaagggtga acttcgacac cttgctcccc
    13561 gccgccttgc tggcattgtg gatggtgatc ttctttctca cataggccgg cacctcctcg
    13621 aaatcctcga tattgtcact catcgactgg gtggagagcc cttcgttcct gataacattc
    13681 tgcatgtgct tcagcttgcg cagtgtcccc acgttcctgt caggcgcctg ctgggcgggg
    13741 cgggcaggag cgggcgaaat atcaatgtca ccgtcaggct cgaaatcaat cactccctgg
    13801 ttgaaggagg cgctgtgagg gggaatggcc ctgtcagagg cacgggtaga ccgcacgata
    13861 tactgaccgt cattctcaga ggcggcgtcg gcgtggcctg gctgcggcgg agctgaattc
    13921 aggctgacaa cctccttcct gaggttgggt ttgactccct ccaggtcagc acactcctcg
    13981 aagccggttg caacaacagt gacactgatg gcatcaccaa gctcctcatc agttgacaat
    14041 cctcttatta tcagcgcatc atcagatacg gtctcatata cataatcagt cagctctcca
    14101 agctcatcca tggtgatctc gttggctccg gtgcccgact tgatgttcag aagcaggctt
    14161 cgtgccccgg tgatgtcact ggagctcagc aacggcgaag taagtgcagc ctcaatagcc
    14221 ctggtggcac gctcctcgcc ggaggcattg cctattccca tcaacgcaac acccgagtca
    14281 cgcatcaccg tgtcaacatc ggcaaaatcg acatttatat aaccggtgca ggtaattatc
    14341 tcggctattc ccttcacggc attcgccagc acatcatcag cccgtgcaaa tgcggccgat
    14401 acgccctcat cgccgtatat ctgcctcaac cgttcattat tgattacaat gagcgaatca
    14461 accttgtctc tcagttcgtt tataccgctt attgccagct tgatacgctg attcagctct
    14521 gagcggaacg ggatggtaac cacggcaacg gtgaggaatc ctgcatcctt gcaggcctgt
    14581 gctatcacgg gagttgcccc ggtaccggta cctcctccca tgcctgctgt gatgaagacc
    14641 atcttggtgt tgcctgaaag cgctttcatg acatcttcca tgttctctat ggcggaagca
    14701 cgacctctct caggctgact tcccgcaccg agaccctcgg tggtggtctc tcccaactgc
    14761 accctgaccg gcaccgggct attcataaga gcctgaaggt cagtgttgca aacgatgaag
    14821 ttgacatcct tgatgccctt ccggtacatg tggttgacag cattgcctcc gccgccacca
    14881 atgcccagga cctttattat tgatgacctg tcaacgggca ggtcaaaatt cattagatca
    14941 tctgacatgg cttcatctta ttttgtaggt gtatcatcaa gtttctcgtc tgtagcctca
    15001 atgatcttat cccatagacc ccgcagcctg tcacccagaa acttgccggt ccgccgttgc
    15061 ccgtcgtgga acagttcatc atgttccacc tctgcaggtt cctctttcac gggcggaggt
    15121 gtatgcatcg gctgctggtg ctctgccacc ggctcctgat ttttcacgcc gatgcgagtg
    15181 aaggttctgg tctcctccag atattcaatc ccctggagta tcaggcccac gctggtggcg
    15241 tacatcggct ggctgatctc atccctgccc tttcctgtga gatgtacgtt gggatgaccg
    15301 atgcgcacat ccattgcggt tttgaacctg accagctgcg gaaggttctt cagcatcgct
    15361 ccgccgccgg tgacaaccac tcctgcggta agtttttcgg cgaagccgga attctgtatc
    15421 tcgtagttga ctgcatcgag tatctcctcc atacggctct ggatgatgta tgccagattc
    15481 ttgaaggaga tttccttggg ctcacgtccg gcgatgccgg gaattgaaac caccttgttc
    15541 tccggagcga tgtcgccaat ggcaaagccg aatttgatct tcaggtcctc ggcctgcttc
    15601 tgcagtatgg agcaaccctc gcggatgtca gcagtaacca tattgccgcc aaacggaata
    15661 acagccgtgt gcctgatgac gttgtcatag tagatggcca catcagtggt tcctccgccg
    15721 atgtcaacca gcaccacgcc tgcctctttc tcttcatcgg tcagcacggc ttcggatgat
    15781 gccagtggct cgagcatgag gctcatcagg ttcattcctg ccttgtttat acacctctcg
    15841 gtgttgcgcg ccgaggcaat ctgccctatc acgatatgga agttggcctc cagcctcttg
    15901 ccgcagatac cgatgggatt gtttataccc gactcgttgt caacgatata ggtctgcggt
    15961 atgacatgaa ttatctcctc acccacatca atggggagct tgtacatgtc agccgtcaga
    16021 cggcgaatgt cgtttatgtc aatctccgtc tcatgggagt cgcgcgtgat ataaccgcgg
    16081 gccatcttgc ttttgatgtg cctgccggcg ataccgacaa agacgtcggt aaggacgagg
    16141 cctgttcttt cttcaaggtc atctacggta gttcgaatgg ccctcaccgt ctcttcaatg
    16201 ttaagcacca caccgcgctt gacccctgta gagggtgtgt tgctaagtcc gagtatctca
    16261 atacccccgt tgccgtcact gcgccctaca atagcaacga tcttcgtcgt gccaatgtca
    16321 atggctgcta ctaactgatc tttattgctc ataattcaat tatatggttg ttcaacaatt
    16381 atcatttcca gttcacataa gtcacactct cctgaatcag tcatccgttt catctatctt
    16441 ctgcgtttta tctatcttct ctggcagaca acctgcccct tgtactcaac atttatcacc
    16501 ctgtacctgt cccaccctgc tacgggcatc gcctggcggt aaaaaacacg gaggttgtgc
    16561 agtttctctt cataattttc aacccgaccc attttcacgg tgtgattgcc tacatggggc
    16621 accagtgtta cccgtccgtc acgactcatg ctgagctggt caatcatccc gttccagaag
    16681 ctgtctccac gtatatagtt caccagctca aaggcttgcg caaggtttgc ggtcttctcc
    16741 gactcaaaaa tgtttgtgcc ggtcatctgc tcggaattaa agaccatatc aatctccagg
    16801 atatgcaggt tgggtgtgta aagattatgc ctcctcatga tgacaccttc gcggtcaatg
    16861 aagaaatcac ctccgtacga tgccatcacc ctcatcaccg gctcccgctg gttaccgtat
    16921 acgtgaagct tcctgtcttc ggagatgtag acttccgcca ccctcagctc ggtgaatgcc
    16981 ttcacccgct cttcgagctt cgtcaagggt atatctctga tcttctgacc ggtaaccctg
    17041 atgcctgtgg ctctcagtgc attcatgatg tcattgttgg taacgaactg gtgctttgac
    17101 gagtcagcaa ggttaatgac cactccctcg catattgcat cagccgtcat tttgctgatg
    17161 aataccgggg taaggaacag cgctgctccg gtaacaatgg tcactattct catcaaaata
    17221 ctcatccgtt cctcctctgt ctcaagcgtt tctattcttc agtatttctg ttaattgtca
    17281 ctttcttcat cagaattctc attcgttata cctctgtctc aagcgtttct gtttttcagc
    17341 atttccgtta tcggttcaac aaatctgtct atatcgcctg cgccgatagt cagcaacagg
    17401 ccctttttca cactattcaa agcaggcaac agctcctcct tcgtcacaac cttcacattc
    17461 cggttcttca tcattcccgc gatcatatcc gaggagacac ccgggattgg catttccctc
    17521 gcgggataga gtgtcagcag tattatttcg tcgagcctgc tgagtgcctc cgcgaagcca
    17581 tcggcgaaat cgcgcgtacg gctgtagaga tgcggctgga aaatgccggt gacatgcttc
    17641 cctggccaga agtcgcgtac ggcactgaca agcgcgttta tctcctgcgg atgatgtgcg
    17701 tagtcatcaa tgtagattat atcattgccg gagtatctga cgtcaaaccg gcggcgcact
    17761 ccacggtaag aaccaacagc gctccgtatg atttcgggtg aaacgcctgc acaccaggct
    17821 gctgcagatg ctgcggtggc attaagtata ttcaccatgc ccggaagatg gatgcggaca
    17881 ttcctgaccg actcaccagg agtgataatg tcaaatgtgt agctcccctc ctccttcctg
    17941 actgattcag ccctgaacga ggctttcgca ccggttccgt aagtataaat gttgacatcg
    18001 cccggaagcg agagcgatga tgcaatgttt tcattgagga tgagtgtccc tccgggacga
    18061 acccggtgac agaactcttc gtaggcttca accatcgact cggctgtgcc gtatatatca
    18121 aggtgatcag gatcaattgc ggtaaccacc gcgatgagag gagagagatg caggaaagac
    18181 ctgtcgaact catctgcctc catcaccgta tagcggcttt cggagagaag caggttggta
    18241 ttgtaattta cggatatgcc accaaggaag gctgaacaac caaccggcga agagctcagc
    18301 caatgggcag tcatagtact cacggtg
//
LOCUS       DAOPBK010000030        18170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3170_length_18170_cov_5.156500, whole genome shotgun sequence.
ACCESSION   DAOPBK010000030 DAOPBK010000000
VERSION     DAOPBK010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3170_length_18170_cov_5.156500"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PLA17_03015"
     CDS             <1..1220
                     /locus_tag="PLA17_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPK84412.1"
                     /translation="GDVWSYRAMIADSTLEGQKPLAESTVTIASLLQRNGYITGAVGK
                     WGLGAPHTDGRPVSQGFDFFYGYNCQRIAHTYYPTHLWRNNHRVWLRNDTLAPHTGLD
                     QGSDPLDPASYEKYSLPDYAPDLMFREILGFIDSCSGKPFFLYWATPIPHLALQAPEE
                     WVNRYHEKFGEEPPYNGNEGYFPVRYPHATYAAMISYLDDQIGSLITHLKERGIYENT
                     IIFFTSDNGPAWSAGTDPEWFASASPFRGSEGYGKGSLNEGGIRVPLIASWPGVTEPG
                     GVTGHLSAFQDFLPTICEITGTEIPGETDGISFLAVLRGGKQKDHDYLYWEFPEYGGQ
                     QAVLIGRYKALRKGMHNGNDAFALYDLENDPQESVDISADHPEIMKRVGEIIRKEHNT
                     SGNPRWRYRLPGE"
     gene            1330..2151
                     /locus_tag="PLA17_03020"
     CDS             1330..2151
                     /locus_tag="PLA17_03020"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPK84413.1"
                     /translation="MSELAEIVEILEGFVPVNLEGIAAVRYMNRVDTKYLFSSGRLPE
                     LLRGVQQHYSALEIESMREFPYKTVYFDTPDLLFYTQHITGKLNRTKVRIRTYESNGL
                     TFLEVKQKSNKGRTCKSRLEKEPGDMNHIEQSREFLNELVSPEAAALKPVITTSFTRI
                     TLVNLERSERITIDYNLAWNNFRGDSLRMPFLAIAEIKSDKSTAMSPFFLMLKEMGVR
                     RTGFSKYAVGMALVNRTERQNNLKPKFLLINRIRDEYYRNDAARHQCSHGRTGNV"
     gene            2087..2788
                     /locus_tag="PLA17_03025"
     CDS             2087..2788
                     /locus_tag="PLA17_03025"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPK84414.1"
                     /translation="MNITGMTLPDISVAMAGQEMFSFAGLPELLLRFCINLVVILVLV
                     RWLYYSTTRRKDYLFTYILISSVVFLLCYLLESVSLQIGFALGLFAIFGIIRYRTNPM
                     PIKEMTYLFLVIGISVINALTAGTSMIEIIFANLAVILITLGLEKVWLLRHISSKSII
                     YEKINLIVPEKYDEMIADLQQRTGIKEIKKVEIGAINFLKDTCRMIIYYEDRGRNVNL
                     ADQNGFIKQDDNDDD"
     gene            2775..3452
                     /locus_tag="PLA17_03030"
     CDS             2775..3452
                     /locus_tag="PLA17_03030"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HPK84415.1"
                     /translation="MTMIRKPAVLLIVMLFSVIAAGQKTDYGIWYEATATKNIRKGLR
                     ADLEASVRTNENAGNIEKFYLEPGLRYKFNDYFNAGVYYRFIGQKEKDGLYHARHRWF
                     IQMKGTAPSVARFTFAVRYRIQQQFRTYHKDPEDKEPQWYQRVRFELDYDVRGIPLKP
                     YINAEMHMQLFNPNDILADKWRSMVGIEYTLNKRHTFGLEYIYNDSKVTKPPYENLLG
                     ITYSVKL"
     gene            3695..4609
                     /locus_tag="PLA17_03035"
     CDS             3695..4609
                     /locus_tag="PLA17_03035"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPK84416.1"
                     /translation="MNKWTTEKIGDLNGKRIVITGSSHGIGYEAAGVLASKGAEVILA
                     VRNREKGEKAAAKIRAMNGSQPVVVMNLDLTDLESVKKFAEEFKSRYDRLDVLINNAG
                     VMVPPYKKTKDGFELQFGTNHLGHFALTGQLLPVLMNTPSSRIVSTSSIAARRAKIHF
                     DNLDGSKGYRPMKFYRQSKYANLLFAIELQNRLKSAGSSTISVACHPGISATNLLSRG
                     KGRETGRVMKALLRLVAQPASMGALPTLCAATHPELKGGEYFGPEGPGNTRGYPVLNN
                     DPARLYNPDISARLWEVSEALTGTRYSI"
     gene            complement(4891..6354)
                     /locus_tag="PLA17_03040"
     CDS             complement(4891..6354)
                     /locus_tag="PLA17_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84417.1"
                     /translation="MIEKSVRIHDKFSLELKFGYSTRRKLKENLFEVNTWIFIPNSLD
                     INRQTYDKSDFYKDLKSNIRLITPEFLLRDIADKTKSPFIHLEASFNALAVTPTRSNA
                     RNYEHQIKMFNSILKSSLRDHRLHIIRNIETEDRDYLISNYLQSSREILSNYRSLRHI
                     IAVPTVRRELFSLYILGDEFMSNLIEQHTFLLADEIRKNHPELYRQTGPMLTELIRGE
                     VEYRRSKGFPTAENLGREEIRELIYRRGTLKKFFDSQLFLATRKKRDGFIAEQLIYSI
                     AAGFAMIFATAIAFIFQKKFGNFTIPLFVALVISYMLKDRIKELTRYYFAGKLKKVFF
                     DHKTRISVTPDKSVGWCKESFDFVSAEKVPADILAIRAQSLDLEVQSVSGWERVVLYR
                     KLMKLDRPSLDGIFTHYTITGVNDIIRFNVSNFVHNMDNPEIPFRILAGDDFEEISGQ
                     KVYYLTFVINVRYDGENQVYLYRVTFNRSGILKVDSL"
     gene            complement(6366..7988)
                     /locus_tag="PLA17_03045"
     CDS             complement(6366..7988)
                     /locus_tag="PLA17_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPK84418.1"
                     /translation="MNSSVKEQEHEEVFDPLDMQNYRIEKLPKRSKGKFEKWLAIAGV
                     PLAIVSFILLAFVFRIPFLSDIDPERLISSAAKKAYEALGAEGFMRTNIMMLAIFVAA
                     LILWITEAIPNYLTSLIIIISLILTKVVPEKEALAQLGHPVMWLNIMSFVLASMLVAT
                     GVAKRFALWFIIKFGKNASSIFISFILINLVLSAFISATTAKAAILLPIFMVIASIYG
                     AHSGTRNNFGRSIVLQNLLNINIGAGAFMTGSGANLLAAALIGGAIGGKVFFGDWMMA
                     MFPIMVGLMFIGYLVAMKIYFPLSPEERLPQIEGGMERLREELKKLGKVDVSEVKAII
                     LFILILGFWATDRLHGISATSVAFVGAVIALLPGIGIMKWNDVDIPWHLMLFSAGAYT
                     LGSGFDYTDLPSIAVNAIFDRMGIGQGTPFWLLYILLTGVMVFSALLFQSKTMRAMIF
                     VPIAIGVANRFGFSVISLALPVAFMIEHVYVLPFNSKPAALLYETDQYSISDAFKFGI
                     TMMIIAWLLIIIAGETWFRFLGITPNGVFGLF"
     gene            complement(8002..8469)
                     /locus_tag="PLA17_03050"
     CDS             complement(8002..8469)
                     /locus_tag="PLA17_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPK84419.1"
                     /translation="MALEIEKKFLVTGDFMKESTKATRITQGYLSSVPERTVRVRIKG
                     DKGYITVKGIGSASGATRFEWEKEIPVPEVEQLLAICEPGVIDKTRHLVVSGKHTFEV
                     DVFHGENEGLVLAEVELSSEDETFVRPGWLGREVTGDSRYYNSMLMKNPYKRW"
     gene            complement(8486..9127)
                     /locus_tag="PLA17_03055"
     CDS             complement(8486..9127)
                     /locus_tag="PLA17_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84420.1"
                     /translation="MAKEELKIGEISKPRFEFRSFGQDFTKAAARMARLSMPVPEKVR
                     ERYSDEIYILSRTNDINNTKIRDGKMDIKTFVQNVDGLEQWNPLMKGEFPMKASVLEG
                     EVFPAFQVSMPRLTKDEYTYDEFMEMVYSHPDLAAVKVHKQRFGYMVNNTICEFGAVL
                     INGAKVYTINSESTEIEDIKKTVSEIGLEGVENINYLQAIKRVIGMINKPLAN"
     gene            complement(9373..10197)
                     /locus_tag="PLA17_03060"
     CDS             complement(9373..10197)
                     /locus_tag="PLA17_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPK84421.1"
                     /translation="MMKTKYFFLLIASLAIFAAGCVEDEVYVGPPSISNMSITPQAPG
                     TTDEVTVTARVTDLKGVTDVKLMYKAGNAAFTEVNMTAGANYQYSGKIPPQAPSTVVQ
                     YYVTASNVSGLTGTLPADAPASTNAYTVGAPSIVINEAFSRGTDAEPDWIEIYNNSDV
                     VVDISGYKIYDNGGQSGSKPKMEFPAKTTIPARGFYVIVVDDKATAYPEGSDFGLGSK
                     GDEVWLETPQGFVIDNMVIPALDLEQSYGSKPDGAATRFVLLERTRGASNNTSPTL"
     gene            complement(10227..11612)
                     /locus_tag="PLA17_03065"
     CDS             complement(10227..11612)
                     /locus_tag="PLA17_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPK84422.1"
                     /translation="MRKPQMISFMLLLAIIIGTGGIVSGQDNDSVRYNEYGLAVKRTP
                     LQAERRNGVLVFESKDQKARVWTDLRVQADGAIFFGEPYNPIGNGTSIRRARMAFKTL
                     FGEHWYGEIDMDISNSELELKDAYLRYSFNKHSVGHGLEIQIGNFKEPFSMETTTTSR
                     YLAFIERANVVATFGPSRHIGIMASYYKNWFLALGGVHFQDIGGLEERTFSKDANKDF
                     GIDEGYSYTGRLVVMPGYANPDFGLHFGMAGSYRTPTTDAEIPGSFRYSTRSLISINR
                     KKYLDTDDIVNVDHALLGGLEFAAYYKGLRMQSEYITNKVNLKNGGVEKFGGFYVYGT
                     WLMFGGKHNYDVTAGEFTQPSRGKKWGDLELAFRYDYIDLNSRQEGIMGGAADGYTIG
                     LNWHANENVKIMLNYAILNHDRYANGKGKLFVGHDIDGNLTKKPSQVVEAAGKAGEDF
                     SFLGIRCEIDF"
     gene            complement(11623..13377)
                     /locus_tag="PLA17_03070"
     CDS             complement(11623..13377)
                     /locus_tag="PLA17_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPK84423.1"
                     /translation="MFFLYLTSGLFLGWSLGANDAANIFGTAVGSKMIRFRTAALVAS
                     IFVIIGATLQGGGTTQTLSQLGSVNALPGAFTVALAAAIVVFLMTKALLPVSTTQAIV
                     GAIIGWNLFTGNSTDVKTLTTILTTWVVSPVIGAIFSALFYILLRALLKKLQLHLIVL
                     DVFIKWALIIVGAFGAYSLGANNIANVMGVFAASAPDVVIDMGLFRLDGLQVLFLLGG
                     IAIALGIITYSKGLMMQVGNGIMALSAEAAIVAVFSQAVVLFIFSSTAFSEFLQGIGL
                     PPIPLVPVSSSQVIVGSIIGIGMIKGVNEIKFKTIKGILYGWVLTPIAAGFLSFFALF
                     FMKNLFNLNVGTEPGAADATADMAVTTGTVVSETTRHINLVWPGISLIVLAVLIIIII
                     YSFSQLKKRLAAEKELLEREKETIIARKALTDAGVRAVQKENLYLQNEIENKRRDLIN
                     YAMNIVEQKEYFGKIADSLRTILSDKKPESREEKLRKLELDIRQRMSFEDKVEDFNIR
                     IERMHKDFIGRLVQLNPDLSPNDIRLAVLLRLGIATKQIATLMNISPKSVEINRYRLR
                     KKLGLDRGSNLTQFINNI"
     gene            complement(13393..14040)
                     /locus_tag="PLA17_03075"
     CDS             complement(13393..14040)
                     /locus_tag="PLA17_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPK84424.1"
                     /translation="MAFFKNATNVINTIETFFNKIDEGVLVFKEGVTNYLHGNQEAFN
                     NNIANMARLEADADNLRRDIENKLYAHSLMPQVRGDILRLLEEMDTIIDTAKSNLFQF
                     DVEVPNIPSELNQDLILLTDMCVSSVAAVIPAARSYFRTPETVKDQIHRVYFYEKEAD
                     KLADSIKRKVFRQMDGLKLSEKFHLRYFTLHIETVSDTAEKVADLLSIMAIKRTM"
     gene            14487..15737
                     /gene="aroA"
                     /locus_tag="PLA17_03080"
     CDS             14487..15737
                     /gene="aroA"
                     /locus_tag="PLA17_03080"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPK84425.1"
                     /translation="MNITISPSGISGTIEAPASKSLTQRAIAAGLLAEGSTVILNPAY
                     CNDSLAAVAMAGELGASVHSEPDRIIIESRRKPTGAVTLNCSESGLALRMFAPISAII
                     TESVKLEGQGSLMRRPVAMIGQALSQLGVTVEATDGRLPLKLTGRLRPGRAFVDGSAG
                     SQLLTGLLMALPLADGESRIDVAGLTSKPYIRLTLSLLAEFGITVENDSFRTFIIPGN
                     QSYRAHEYTVEGDWSSAAFLLVAGAMAGRVTVNGLNLHSVQADRAILTVLNDAGAALF
                     AAPDAVTSSLSGLHSFTFDATDSPDLFPPLAALAAGCEGTSSIRGVSRLRHKESDRAV
                     AIADVLEAMKIRVKISGDEMLITGGRVQGAVVSSHNDHRIAMMASVMAASATAPVTVA
                     GAEAVAKSYPGFYNDMGRLGAVIF"
     gene            15902..17026
                     /locus_tag="PLA17_03085"
     CDS             15902..17026
                     /locus_tag="PLA17_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPK84426.1"
                     /translation="MITGFRQKDIEEIFCTPIVQQTAFWSEVKRRMGISSIACNFMVA
                     EDDIKPQGEANRKITSDLLVVMRQIDPNHSIAYVPYGPELEPADEFRGVFLEELSECL
                     RSHLPADCFLIRYDLAWESFWAGDEETCTPEGLWPGPPAYPLQELRFNFSTRNMNFRK
                     AQSNILPSNTVFLDLLRDEASLLKSMKSKTRYNIGLALRKGVTVSVAGPEQLERWYAL
                     YRETACRNNFFLHDIEHFRAVLSSRAENTQSPAEVCLLIAEHDKDPLAAMFLVITGNR
                     GTYLYGASSSHKRNLMATYALQWKAITLAKEKGCSEYDMFGISPGPDPDHPMYGLYRF
                     KTGFGGEIYHSMGCWDYPIDNRVYSYFAASELTRQGYHLS"
     gene            17389..>18170
                     /locus_tag="PLA17_03090"
     CDS             17389..>18170
                     /locus_tag="PLA17_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPK84427.1"
                     /translation="MKQLIMIMLIVLLCSFQAALSQRSISGKVTDASDGVALPGVTVI
                     VKGLTVGTSTDTDGNYRLSVPAGSNTLVFSMIGYHDHEVILGASNTCDVALEPATELI
                     EDVVVTALGIKRDKKALGYSVSSIKAAEITVAGTPQNALASLYGKAAGVGIQLGTAGP
                     TGGVNIKIRGSAGLDVSTKTRPLFVVDGVPIFDENSNMANRGYDPLNSPDYGTGINDI
                     NSEDIESIEILKGAKATVLYGSRAGNGVVMITTKKGQATRGLG"
BASE COUNT         4723 a         4307 c         4397 g         4743 t
ORIGIN      
        1 ggggagatgt ctggagctac agggcgatga tagccgattc cacactggag ggtcagaagc
       61 cgctggcaga gagtaccgtc accatcgcct ccctgctgca aagaaacgga tatattaccg
      121 gtgctgtagg caaatggggc cttggtgcgc cacatacgga cggacgccct gtcagccagg
      181 gatttgactt cttctacgga tacaactgcc agaggattgc ccacacttac tatcctacgc
      241 atctctggcg caataaccac agggtatggc tgaggaacga cactcttgcc ccgcatacag
      301 gacttgatca gggttctgac ccgcttgacc cggcctccta tgagaaatac agtctccctg
      361 attatgcgcc ggatctgatg ttcagggaga tacttggttt cattgactcc tgttccggaa
      421 aacccttttt cctttactgg gcaactccca taccccatct ggctctgcag gcgcctgagg
      481 aatgggtaaa caggtaccat gagaagttcg gtgaggagcc tccctacaat ggcaatgaag
      541 gctatttccc tgtaaggtat ccccatgcca cctacgctgc aatgatctca taccttgatg
      601 atcagattgg cagcctcatc actcacctga aggagagggg catttatgag aataccatta
      661 tcttctttac cagtgacaat ggtccggcat ggagcgcagg aactgatcct gaatggtttg
      721 caagcgcttc ccccttcagg ggatcagagg gttatggcaa aggttcactc aacgagggtg
      781 gtatcagggt tccgctgatc gcctcatggc ccggggtgac tgagccggga ggtgtcaccg
      841 gccatctctc tgcctttcag gacttccttc cgacgatatg cgagataacc ggcacagaga
      901 tacccggcga aactgacggc atcagtttcc ttgcggtact ccgcggagga aaacaaaaag
      961 accatgacta tctctactgg gagtttcccg aatacggagg gcagcaggcc gtgctcatcg
     1021 ggaggtataa ggcgctccgc aagggaatgc acaatggcaa tgacgctttt gcactctatg
     1081 accttgagaa tgacccgcag gagagtgttg atatttcagc agaccatccg gaaatcatga
     1141 agagggtcgg ggagataatc cggaaagagc acaatacctc agggaatccg cgatggaggt
     1201 acaggctgcc gggagaatga cacatggtga aataatgccg gcttccggaa cgttatagtt
     1261 taaatgcacc ggcatgggaa aagagctttc ttcctgcacg aagtcagctc tctttacgcg
     1321 gtttccggaa tgagtgaatt ggcagaaata gttgaaatac ttgaaggatt tgtgccggtg
     1381 aacctggagg gtatcgccgc cgtgaggtac atgaaccgtg ttgacaccaa gtacctcttt
     1441 tcatcaggca ggcttccgga gcttctccgc ggggtgcagc agcactacag cgccctcgag
     1501 attgaaagca tgcgggagtt cccttacaaa acagtatatt ttgacacgcc tgacctgctg
     1561 ttctataccc agcacatcac cgggaagctc aatagaacaa aggtcagaat caggacttac
     1621 gagtcgaacg gactgacctt cctggaggta aaacagaaat cgaacaaagg ccgcacatgt
     1681 aaatcacggc ttgaaaagga gcccggtgat atgaatcaca ttgagcagtc aagggagttc
     1741 ctcaatgaac ttgtctcccc ggaagccgcg gcgctgaaac ctgtcatcac cacctccttt
     1801 acccgcatca cccttgtgaa cctggaaaga tccgagagaa taaccatcga ttataacctg
     1861 gcatggaaca acttcagggg tgacagcctg aggatgccct tcctggcaat agctgagatc
     1921 aagagtgaca agtcaacggc aatgtctcca ttctttctca tgctcaagga gatgggggta
     1981 cgcaggacag gattcagcaa atatgcggtt ggtatggccc tggtgaacag gacagagagg
     2041 caaaataatc taaaaccgaa gtttttatta attaacagga tcagggatga atattacagg
     2101 aatgacgctg cccgacatca gtgtagccat ggcaggacag gaaatgttta gcttcgcagg
     2161 attaccggag ttgttgctgc gtttctgcat caacctcgtg gtgatactgg ttctggtgcg
     2221 ctggctttat tacagcacca ccaggagaaa ggattacctc tttacctaca ttctcatttc
     2281 cagcgtggtt ttcctgctct gctacctgct tgagagtgtc agccttcaga tagggtttgc
     2341 acttggcctg ttcgcaatct tcggcataat cagataccgg acaaacccga tgcccatcaa
     2401 ggagatgaca tacctcttcc tggtgatagg tatttcggtg atcaatgccc ttactgcagg
     2461 cacaagcatg attgagatca tctttgccaa cctggccgtg atactcatca ccctgggact
     2521 tgagaaggta tggttgctga ggcatatctc ttcaaaatcg atcatatatg aaaagatcaa
     2581 cctgatagtg ccggagaagt atgacgagat gattgctgac ctgcagcaga gaacagggat
     2641 caaggagata aagaaggttg aaataggtgc gattaatttt ctgaaggata catgcaggat
     2701 gataatctat tacgaagaca ggggaagaaa tgtaaacctg gctgaccaga acggattcat
     2761 caaacaggat gacaatgacg atgattagaa agccggcggt tctgcttatt gtgatgctgt
     2821 tctccgtgat tgcggcggga cagaaaactg attacggcat ctggtatgaa gccactgcta
     2881 caaagaatat ccggaagggg ctcagggctg atcttgaagc cagcgtcagg accaatgaga
     2941 atgccggtaa tatagaaaag ttttacttag agcccggatt gaggtacaag ttcaatgact
     3001 acttcaatgc aggagtttat tacaggttta tcgggcagaa ggagaaggat ggtctgtacc
     3061 acgcgcgtca ccggtggttt attcagatga agggcactgc tccatcagta gcccgcttca
     3121 cctttgcagt caggtaccgt atccagcagc agttcagaac ttaccataag gatccggaag
     3181 acaaggaacc gcaatggtat caaagggtaa gattcgaact tgattatgat gtcaggggta
     3241 tcccgctgaa gccctacata aatgcggaaa tgcatatgca gctttttaac cccaacgata
     3301 ttcttgctga caagtggagg tcaatggtgg ggatagaata tacgctcaac aagaggcata
     3361 ctttcggact cgagtatata tacaatgaca gcaaggttac aaaaccgcct tacgagaacc
     3421 ttcttggcat tacctattca gtgaagcttt agtttcattc cgtcagagtc acttttaatc
     3481 tgttcacgga cagctgttac gtaccttcag tgccatcgtt taatgatgtc aggtaagttg
     3541 ttccgtcgcc gggtgctggt ttgaagttgt cagacataac cttaatcagt agtcttaatt
     3601 cagggagtat tatagtggtg caaatcgatg atttgcctta tatttacagc aggttttcag
     3661 ccccggactg tttttatttt ttaaaaaaac tgacatgaat aaatggacta ccgaaaaaat
     3721 tggagatctt aacggaaaga ggatagtaat tacaggatca tcacacggga taggttatga
     3781 ggcggccgga gttctggcat caaagggtgc tgaggtgatt cttgcagtaa ggaacaggga
     3841 gaaaggggag aaggccgctg caaagatcag ggccatgaac ggatcgcagc ctgtggttgt
     3901 gatgaacctc gacctgacgg accttgaatc ggtaaagaaa tttgcagaag agtttaaatc
     3961 ccgttatgac aggcttgatg tgctgatcaa caatgccgga gtgatggttc caccctataa
     4021 aaagacaaag gatggctttg aacttcaatt cggcacaaac catctcgggc actttgcact
     4081 gaccggtcag ttgctgccgg tgctgatgaa cacaccgtcg tcgcgcattg tctccaccag
     4141 cagtatagct gcgaggaggg caaagattca ctttgacaat ctggacggtt cgaaaggata
     4201 cagacctatg aaattttacc gccagagcaa atatgccaat cttctgttcg ccattgagtt
     4261 gcagaacagg cttaagagtg cagggtcatc aaccatcagc gttgcctgtc atccgggcat
     4321 cagtgccacg aatcttctct cccgcggcaa gggaagagag accggcaggg tgatgaaggc
     4381 actcctgcgg ttggtagccc agccagcttc gatgggagca ttgccaacac tctgcgctgc
     4441 cactcatcct gaactgaagg gaggtgagta tttcggtccc gagggcccgg gtaataccag
     4501 gggttatcct gtgcttaaca atgacccggc aagactgtac aatccagaca tctctgccag
     4561 gctgtgggag gtgtcggaag cattgacagg aacccggtat agcatctgag gagaatcagc
     4621 aggttatctg gaattagccg caggctgtaa tgctttgcat aacctggagt gagcctgcca
     4681 caaggtattg gccccggcct gccaggcatt gcataacctg gagtgagcct gccacagggc
     4741 attggccccg ggtcataaaa gttgcgggct catggaaacc ttaccacagg gaggcagtga
     4801 caatgctggt aacgctacgg ccggcaagta tcatacacgg ctatttggat caccagaaat
     4861 gtaatgattt cacaatagta agataaagtg ttacagggaa tcgaccttca ggatacctga
     4921 cctgttgaaa gtaaccctgt agaggtagac ctggttctcg ccgtcatatc tgacattgat
     4981 gacaaatgtg aggtaataga ctttctgacc gcttatttcc tcaaaatcat ccccggcaag
     5041 tatcctgaac ggaatctccg ggttgtccat attatgcaca aagttagaga cattgaacct
     5101 tataatgtca ttgacaccgg ttatcgtata atgtgtgaaa atgccatcaa gtgatggtct
     5161 gtcaagcttc atgagcttcc tgtaaagcac caccctttcc cagccgctga cactctggac
     5221 ctcaagatca agtgactgag ccctgatagc gaggatatca gccggcacct tttcagctga
     5281 gacaaaatca aagctctcct tgcaccagcc aactgattta tccggggtga cggagattct
     5341 tgtcttatga tcaaagaaaa ccttcttcag ctttccggcg aagtaatacc tggtaagctc
     5401 cttgatccgg tccttgagca tgtagctgat aacaagggca acgaagagcg gaatggtaaa
     5461 gttaccgaac ttcttctgga atataaatgc gatagccgtt gcgaagatca ttgcaaaacc
     5521 ggcggcaatg ctgtaaatga gctgttcagc aatgaatccg tctctttttt ttctggtggc
     5581 gaggaacagc tggctgtcga agaacttctt cagagtgccc cgtctgtaga tcaactccct
     5641 gatctcctcc ctgcccaggt tctcagccgt ggggaagccc tttgatctcc ggtattcaac
     5701 ctccccgcgg atgagttccg taagcatcgg gcctgtctgt ctgtacagct ccggatggtt
     5761 cttcctgatc tcatcagcca gcagaaaggt atgctgttca atgaggttac tcatgaattc
     5821 atcaccgagg atgtatagcg agaagagctc tctgcgcacc gtgggcaccg caattatatg
     5881 cctcaggctc ctgtaattgg aaagtatttc acgtgaactc tgaaggtaat tggatattag
     5941 atagtcacga tcctctgtct caatgttccg gatgatgtgc aggcggtgat cacgaaggga
     6001 tgacttgagg attgagttga acatctttat ctggtgctca taattccggg cattactacg
     6061 tgtaggagtg acagccagtg cgttgaatga agcctcaaga tgaatgaatg gcgactttgt
     6121 cttgtccgct atgtcacgta aaagaaactc cggggtgata agcctgatgt tcgatttcag
     6181 gtctttgtag aaatctgatt tatcataggt ttgcctgttg atatccaggc tgttgggaat
     6241 gaatatccat gtattgacct cgaaaaggtt ctccttcagt ttcctcctgg ttgagtaccc
     6301 gaatttaagc tcgagggaga atttatcatg tatcctgaca ctcttttcga tcatctccgg
     6361 tcatatcaga atagtccgaa aactccgttc ggcgttatgc caaggaacct gaaccaagtc
     6421 tcacctgcga taatgatgag cagccaggca atgatcatca ttgtgatacc aaatttgaaa
     6481 gcgtcggaga tgctgtactg gtctgtctca tacaacagtg ctgcaggttt gctgttaaag
     6541 ggaagaacat agacatgctc aatcatgaaa gctaccggca gtgcaaggct gataacgctg
     6601 aagccgaatc tgttggcaac accaatggca atgggaacaa atatcattgc cctcattgtc
     6661 ttcgactgga acagcagggc gctgaatacc atgacaccgg ttagcagtat ataaaggagc
     6721 cagaacggag ttccctgacc tatacccatg cggtcgaaaa ttgcattgac ggcaattgag
     6781 ggcaggtcag tataatcaaa gccggaacca agagtatatg cacctgccga gaagagcatc
     6841 aggtgccatg gtatatccac atcattccac ttcataattc ctatccccgg gaggagggca
     6901 atgaccgctc ccacgaacgc cacggaggtg gcacttatcc catgcaggcg gtcggtagcc
     6961 cagaatccga gtatgaggat aaaaagaatg atagccttga cttcacttac atctacctta
     7021 cccagcttct tcagctcttc gcgaagcctt tccattccgc cctcgatctg tgggagcctc
     7081 tcttcgggag agaggggaaa atagattttc atggcaacca gatagccgat gaacattaac
     7141 ccgaccatta tcggaaacat cgccatcatc cagtcgccaa agaacacctt tcctccaata
     7201 gcccctccga taagtgcggc ggcgagcagg ttggctcctg agcctgtcat gaaagccccc
     7261 gctccgatat tgatattcag aaggttctgc aatacaatgc tgcgcccgaa attattcctc
     7321 gtacctgagt gtgccccgta gattgaggct atgaccatga atatgggaag tagtatggca
     7381 gcttttgccg tggtggcaga aatgaatgca gagagcacca ggttaatcag aatgaagctg
     7441 ataaagattg atgatgcatt cttcccgaat ttaataatga accacagtgc aaatcgtttt
     7501 gccacgccag tggcaaccaa catgctggcc agcacaaatg acatgatatt cagccacatg
     7561 accgggtgtc cgagctgtgc aagagcctcc ttttcaggaa ccactttggt gaggatgaga
     7621 ctgattatta tgatcagaga ggtaagataa tttggaatgg cctcggtgat ccacagaatg
     7681 agagcagcga caaagatcgc cagcatcatg atattcgtcc gcatgaagcc ctcagcgcca
     7741 agcgcttcat aggctttctt tgcagcagaa gagatgagcc tttcaggatc aatgtcactc
     7801 aggaacggaa tcctgaagac aaatgccagc aatatgaaac ttactattgc aagaggcact
     7861 cccgctatgg caagccattt ctcgaactta ccctttgatc tcttcggtag cttttcaatc
     7921 ctgtagtttt gcatgtcaag aggatcaaac acctcttcat gttcctgctc ttttacagac
     7981 gaattcatat cctgtcagtt attaccagcg tttgtaagga tttttcatca acattgaatt
     8041 gtaatacctg gaatcacctg tcacttcccg gccaagccag ccgggtctga caaatgtctc
     8101 atcctctgag gagagttcca cctcggcaag tacaagtcct tcattttcac catgaaaaac
     8161 atcaacttcg aaggtgtgtt tgcctgatac aacaaggtga cgggttttgt caataactcc
     8221 gggttcacat attgctagaa gctgctcgac ttccggaacg gggatttcct tttcccattc
     8281 aaagcgtgtt gctcctgagg cgctgccaat acccttcacg gtaatgtagc ccttgtcacc
     8341 cttgatcctt accctgactg ttctttcagg aactgagcta agatagccct gggtgatcct
     8401 ggttgccttt gttgattctt tcatgaagtc gcctgtcacc aggaacttct tttctatttc
     8461 aagtgccatt tttgttctgt tggtttcagt ttgccagtgg tttgttgatc ataccaatga
     8521 ccctttttat tgcctgcagg tagttgatgt tctccacccc ctcgaggcca atctcactga
     8581 ccgttttctt tatatcctca atctccgttg attctgagtt aattgtataa acctttgcgc
     8641 cgtttatcag tactgcacca aattcacaga ttgtattgtt caccatatat ccgaaacgct
     8701 gcttgtgaac cttcactgct gcaagatcag gatgactgta aaccatttcc atgaattcat
     8761 cataggtgta ttcatctttg gtcagtctgg gcatgcttac ctggaatgca gggaagacct
     8821 ctccctcaag aacggatgcc ttcatgggaa actcaccttt cataagcgga ttccactgct
     8881 cgaggccgtc aacattctgc acaaaggtct taatatccat cttgccgtcg cgaattttgg
     8941 tgttgttgat atcattggtc cgcgacagta tataaatctc gtcagaatat ctctcccgga
     9001 ctttttccgg tacgggcatt gacagccgtg ccatgcgggc tgcagctttt gtaaagtcct
     9061 gtccaaatga tctgaattca aatctcggtt tcgatatttc tcctattttg agctcttctt
     9121 ttgccataca agtaaatttt gtttggtaat gtcaatggtt cggtttattt ttcagatgat
     9181 cactcatata actaccagat tatcagcatt gatcatcttt ctaagatgag cgcctgatta
     9241 atgagctata tgtctgccag caaaaagcta ccgacctgtt attaatttaa aacaacggtt
     9301 tatgatatgg tgaaccatga aataaaggta atcaccggcg ggggaagagc cggtgattac
     9361 cgttttccgc catcagagtg tcggtgaggt gttgtttgat gctcccctgg ttctctcaag
     9421 caggacaaac ctggtagctg cgccgtcagg tttgcttccg tatgactgtt cgagatcaag
     9481 agccgggatc accatattgt caatcacaaa tccctggggt gtctcgagcc atacttcgtc
     9541 accttttgat ccgagaccaa aatctgaacc ttcaggatat gcagttgctt tatcatcaac
     9601 cacaatcacg taaaatcccc tggcagggat tgttgttttg gcagggaact ccatcttggg
     9661 tttcgagcct gactggccgc cattatcata aatcttataa cctgagatat ctacaacgac
     9721 atctgagttg ttatagatct ctatccagtc aggttcagca tcggtacctc ttgaaaaggc
     9781 ttcgttgatt actattgatg gtgcgccaac cgtataggcg ttggttgagg caggagcatc
     9841 ggcagggagg gtccctgtca gtcctgatac gttggaagca gttacataat actgaactac
     9901 cgtggagggc gcttgtggtg gaattttgcc actatactgg tagtttgcgc cggcggtcat
     9961 gttcacctcg gtaaaggcag cgtttccggc cttgtacatc agcttgacat cagtgactcc
    10021 cttcaggtcg gtcacccttg ctgtcaccgt tacctcatca gttgttccgg gtgcctgcgg
    10081 tgtgattgac atatttgaaa tggacggggg gccaacatat acctcatctt ccacgcatcc
    10141 tgcagcaaag atggcaagtg aggctatcaa cagaaaaaag tattttgttt tcatcatatt
    10201 attgttttga tttttacggt atgaaattag aagtctatct cgcagcgtat gcccaggaag
    10261 ctgaaatcct caccggcctt gccggctgcc tcaacaacct gtgaaggttt ctttgtcagg
    10321 tttccgtcta tgtcatggcc gacgaacagc ttgccttttc cgttggcata cctgtcatgg
    10381 ttcaggatag cgtaattgag cattattttc acattttcgt tggcatgcca gttgagtccg
    10441 atggtatatc cgtcggctgc gccgcccata atgccttcct gcctgctgtt gaggtcaatg
    10501 tagtcatacc tgaatgcaag ttcaaggtca ccccatttct tgcctcttga aggctgggtg
    10561 aactcaccgg ctgtgacatc atagttatgc tttcctccga acatcagcca ggtgccataa
    10621 acatagaatc ctccgaattt ttcaacaccg ccgtttttaa ggttcacctt gttggttata
    10681 tactcactct gcattcttaa tcccttatag taggcagcaa attcaaggcc gcccaacaga
    10741 gcatggtcaa catttacaat atcgtctgta tcaagatatt tcttcctgtt gattgatatc
    10801 agtgacctgg tgctatacct gaatgaaccc gggatctcag catcggtagt gggggtgcgg
    10861 tatgaacctg ccattccaaa gtgaaggcca aaatcaggat tggcatagcc tggcatcaca
    10921 acgagcctgc cagtatagga ataaccctca tctataccaa agtctttgtt ggcgtctttt
    10981 gagaaggtcc tttcttcaag tccgccgata tcctggaaat gaacgcctcc cagtgcaagg
    11041 aaccagttct tgtagtagct ggccataatc ccgatatgtc ttgacgggcc aaaagtggca
    11101 accacgtttg ccctttcaat gaatgccagg taccttgatg ttgtcgtagt ctccatggag
    11161 aagggttcct tgaaattacc tatctgaatc tcaaggccat gaccaaccga atgtttattg
    11221 aagctatacc tcaggtaggc atccttgagc tccagttccg aattggagat gtccatgtcg
    11281 atctcaccgt accaatgctc cccgaagagg gttttgaaag ccatgcgggc tcttctgatc
    11341 gaggtcccgt taccgattgg attgtatggc tccccgaaga atatggctcc gtcagcctgt
    11401 acccgcaggt cagtccagac acgtgctttc tgatcctttg attcaaagac caggactccg
    11461 tttcttctct ccgcctgcaa tggcgttctt tttacggcaa gaccgtactc attgtaacgg
    11521 acagaatcat tgtcctgccc cgaaactatg ccgcctgttc ctattataat ggcgagcagc
    11581 agcataaaac tgatcatttg tggttttctc ataattgatt tgttaaatgt tatttataaa
    11641 ctgtgttaga ttggatcctc tgtcaaggcc tagttttttg cgcaaacggt atctgtttat
    11701 ctctacgctc tttggagaaa tattcattaa tgtggcaatc tgcttggtgg caattcccag
    11761 ccgcagcaat actgccagcc tgatatcatt gggggagaga tcggggttga gctgcacaag
    11821 tcttccgatg aaatccttat gcatcctttc aatcctgata ttgaaatctt caaccttgtc
    11881 ttcaaagctc attctttgtc taatgtccag ttccagcttc cggagcttct cttcccttga
    11941 ttcaggtttt ttatcgctga gaattgtcct gagagaatct gcaattttgc caaagtattc
    12001 tttttgttca actatgttca ttgcataatt gataaggtct cttctcttgt tttctatttc
    12061 gttctgcagg tacaggtttt ccttctgcac tgcacgcacc ccggcatctg tcagggcttt
    12121 ccttgctata attgtctcct tctccctttc cagtaattcc ttttctgctg ccagtcgctt
    12181 tttcagctga ctgaatgaat agattataat aattataagt accgcaagaa caatcaggga
    12241 tatccccggc cagaccaagt taatgtgcct tgttgtttct gataccactg tgcctgttgt
    12301 cactgccata tcagcagtcg catccgctgc tccgggttct gtaccaacgt tgaggttaaa
    12361 aaggtttttc atgaagaaga gggcaaagaa tgaaagaaac cctgcagcaa tcggggtaag
    12421 cacccacccg tatagaatgc cctttattgt cttgaatttg atctcattta ctccttttat
    12481 cattccaatg ccgataattg accccactat tacctgtgag gaagaaaccg gtaccagcgg
    12541 tattggtggc agtccgatac cctgcaggaa ttcggaaaaa gccgtcgatg aaaagatgaa
    12601 tagcacaaca gcctgtgaaa atacggcaac aatggctgcc tcagcagaga gggccatgat
    12661 tccgttgccc acctgcatca tcagcccctt gctgtatgtg atgattccca gggctattgc
    12721 tataccacct aggagaaaaa gtacctgcag gccgtcaagt ctgaacaagc ccatgtcaat
    12781 aaccacatcg ggcgccgaag cagcaaatac acccattacg ttggcaatat tgtttgcccc
    12841 aaggctgtaa gccccaaatg cacctactat gataagagcc catttgatga atacatcaag
    12901 cacaataaga tgtaactgaa gtttcttgag cagagccctg agaagaatgt aaaagagagc
    12961 ggagaaaata gctccgatta ccgggcttac cacccatgtg gtcagaattg ttgtcagagt
    13021 cttaacatca gtgctgttcc cggtaaagag attccatccg attatggctc cgactattgc
    13081 ctgtgtagta gaaacaggca gcagagcttt tgtcataaga aaaaccacta ttgcagctgc
    13141 cagagcaact gtaaaggcgc ccggcagggc attaaccgaa ccaagctgac tgagtgtctg
    13201 cgtggttcca ccgccctgaa gcgtggctcc tataatgaca aaaatgctgg caaccaatgc
    13261 agcggtacgg aatctgatca tctttgaccc gacggcagtg ccaaaaatgt tggccgcatc
    13321 attggctccc aatgaccatc cgagaaaaag tccgctggtt aagtaaagga aaaacactgc
    13381 ctgtgacttg ctttacattg tacgtttgat agccattatt gaaaggagat cagcaacctt
    13441 ctccgccgtg tcagatactg tctctatgtg cagggtaaaa taacgcagat ggaacttctc
    13501 acttaacttg aggccgtcca tctgcctgaa taccttcctc ttgattgaat cagccagctt
    13561 atcagcctcc ttttcataaa aatataccct gtgtatctga tccttcactg tttccggagt
    13621 cctgaagtaa gacctggctg ccgggataac cgcagcgact gatgagacac acatgtcagt
    13681 aagcagaatg agatcctggt tcaactccga cggaatattc ggaacttcaa catcaaactg
    13741 aaaaagattg ctcttggctg tatcaataat agtgtccatc tcctcaagca acctcaatat
    13801 gtcacccctg acctgtggca ttagtgagtg ggcataaagc ttattctcta tgtcacgcct
    13861 cagattgtct gcatcagctt ccagcctggc catattggct atgttgttgt taaaagcctc
    13921 ctgattgcca tgcagatagt ttgtaacacc ctccttgaag acaaggacac cttcgtcaat
    13981 cttattgaag aaagtctcga tcgtattgat cacattggtt gcattcttga aaaaggccat
    14041 cgtgtaacac tatagtttga cggcaaaggt aaaccattaa ctttactctg agcactattt
    14101 gcggttgtag tttaactaca ggcaagtcat ggatgtatag agtttcgtta aatattgtat
    14161 taactgtata tcaattgttt atattcgatt aaaaggctgt atgtagtgtt atattatcag
    14221 taacactgat ttttgtagag gccttgtata gaaatttaaa agcataaaat caggactgaa
    14281 agcctctaca tgaaacatgg ttcatgcctt gcgaaacgat acaaaccagt caggtggaca
    14341 ggggagtcag aaagggtctg acacccgggt ttatcgaatc agttccggaa acgagtcacc
    14401 gggaatctgt caaataccag attaaagtga tgaaagatga gtagatttgt gtttaagcca
    14461 ggccgcagag gcctcatcat gagtctatga acattacaat aagtccctca ggaatcagtg
    14521 gaacaattga agcgcctgca agcaagagtc ttacgcagag agccatagct gcggggttgc
    14581 ttgcagaagg gagcacagtt atcctgaatc ctgcatactg caatgattcc ctggcagcag
    14641 ttgcaatggc cggcgaactg ggagcatcgg ttcacagcga accggacaga ataatcattg
    14701 agtcgcgaag gaagccaacc ggagcggtta cacttaactg cagtgagtca gggctggcat
    14761 tgcgtatgtt tgcccccata tccgctatta taacggagag cgtaaaactc gaagggcaag
    14821 gttcgctcat gcgcaggcct gtggcaatga tcggtcaggc gctgagtcag ctgggtgtga
    14881 ccgttgaagc aactgacggg agactgccat taaaacttac cgggcgtctc aggccaggcc
    14941 gggcatttgt tgacggttct gccggatcac agctgctgac agggctgctg atggcacttc
    15001 cccttgctga cggggagagc aggattgatg tggcaggtct gaccagcaag ccatacatca
    15061 ggcttaccct cagtctgctg gctgagttcg gaatcactgt tgagaatgac agcttcagaa
    15121 cattcataat ccccggaaat cagtcataca gggctcatga gtacaccgtg gagggcgact
    15181 ggagcagtgc ggcgttcctt cttgttgccg gcgccatggc cgggagggtg acagttaatg
    15241 gtctgaatct ccatagtgtg caggctgaca gggcaatact aactgtactt aatgatgctg
    15301 gcgctgcact gtttgccgct cctgacgctg ttacatcgtc tctctcagga cttcactcct
    15361 tcacttttga tgccaccgat tctcctgatc tttttccacc tctggcagcg ctggcagccg
    15421 gttgtgaggg caccagcagt atcaggggtg tgagcaggct gaggcacaag gagagcgaca
    15481 gggcagtggc aattgccgat gtgctggagg caatgaagat cagagtaaaa atcagcgggg
    15541 atgagatgct catcacggga ggcagggttc agggagctgt tgtctcttcg cataatgacc
    15601 accgcatagc gatgatggcc tccgtaatgg ctgccagcgc cacggctccc gttactgttg
    15661 caggagctga ggcagtggca aaatcatacc ccggattcta caatgacatg ggccgcctcg
    15721 gagcggtaat attctgatca gacggaagcc gggttgtgga ttgcgccatt accggaacct
    15781 ccctgtcagg gaggctgttt cggctcaaat caattcctta agaaatatcc ttggtattct
    15841 gattattaag tctcatgttt atggcgccgg caaaaggcag gcccttatcc agtcttatat
    15901 catgattacc gggttcagac agaaagacat agaggagatt ttttgcaccc cgatagtgca
    15961 gcagactgca ttctggtcgg aagtgaagcg ccgcatgggc atcagttcca tagcctgtaa
    16021 cttcatggta gctgaagatg atattaaacc acagggcgaa gcgaacagga aaatcacctc
    16081 cgatctgctt gtggttatga gacagatcga tccgaatcat tccattgcct atgtccctta
    16141 cggtccggaa ctggaaccgg ctgacgagtt caggggagtc ttcctcgaag aactttcaga
    16201 gtgtctccgt tcgcacttgc cggccgactg ttttttgata aggtatgatc tggcgtggga
    16261 gtcattctgg gcaggcgatg aggagacatg tacgcctgaa ggtctctggc ctggccctcc
    16321 agcatatccc ttgcaggaac tgaggtttaa tttctccacc cggaatatga atttcagaaa
    16381 ggctcagtca aatattcttc cgtcaaatac ggtttttctg gacctcctca gggatgaggc
    16441 ttcattgcta aagtcaatga agtccaagac caggtacaat atcggtctgg cactgcgcaa
    16501 aggagtaact gtaagcgttg cagggccgga acaactggag agatggtatg ccctttaccg
    16561 cgagacagcc tgcaggaaca atttttttct gcatgacata gaacatttcc gtgctgttct
    16621 cagcagccgg gcagaaaaca cacagtcacc cgccgaggtc tgtctgctca ttgcagaaca
    16681 tgataaggat cctctggcag caatgttcct ggttataacg ggaaacaggg gaacatatct
    16741 ttacggagcc tcatcgtcac acaagcgcaa tctgatggca acctatgccc tgcagtggaa
    16801 agcaatcact ctcgcgaagg agaagggatg ctctgaatat gacatgtttg gcatctctcc
    16861 aggccctgac cctgatcacc ccatgtacgg gctttaccgg ttcaagaccg gtttcggagg
    16921 agagatttac catagcatgg gatgctggga ctatccgatt gataacaggg tatattcata
    16981 tttcgccgca agcgaattaa cgcgtcaggg atatcacctc tcctgaatct gtaactgcat
    17041 tttcatcata atctaatcga gccggcatca gtggaaatta cataaacagg tagttctgtt
    17101 ttgccacatg tgcgtttcat atttttttgc attaaatcat acataatact tgcatttttg
    17161 tgaaggacat aataaatttg tatcaggctt gtgtctgatt atatctgata attaaaacgt
    17221 tttcggttca gataatgctt ctactaaacc cagaaataac cctgataaac ctgcacatta
    17281 tgaaaatgac aatttgcaat tctccctgag caggttcctg ccggtattat accggccctg
    17341 caagattcag ttaacaaaca gcccgttttt acatctaaaa tctagcttat gaaacaacta
    17401 atcatgatca tgttgatagt gttgctatgc agttttcagg cagcgctgtc acaaagaagc
    17461 atttccggaa aggtgacgga tgccagcgat ggtgttgccc ttcccggtgt aaccgtcatt
    17521 gtaaagggac tgaccgttgg gacctctacc gatacagacg ggaactacag gctatcggtt
    17581 ccggccggca gcaataccct ggttttttcg atgatagggt atcatgacca cgaggtaatt
    17641 ctcggggcgt ccaacacctg tgatgttgca cttgaaccgg ccacagagct aatagaggat
    17701 gttgtagtca cagctcttgg catcaagcgt gacaagaagg cactaggtta ttcagtcagt
    17761 tcaatcaagg ctgctgaaat aactgttgcc ggaacgccac agaacgccct tgcctcgctc
    17821 tatggcaagg ctgccggtgt tggtatacag ctaggcaccg caggccctac cggcggagta
    17881 aacataaaga ttcgtggttc cgcaggcctc gatgtcagta caaagacccg gccgctgttc
    17941 gtcgttgacg gagttccgat ctttgacgaa aactccaaca tggcaaacag gggttatgac
    18001 ccgctcaact caccggacta cggtaccggg ataaatgaca tcaacagtga ggatatcgaa
    18061 tcgattgaga ttctcaaggg tgccaaggcc accgtacttt acggaagccg tgccggtaac
    18121 ggagttgtga tgatcacaac caagaagggg caggctacca ggggtctcgg
//
LOCUS       DAOPBK010000031        17982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3226_length_17982_cov_5.000725, whole genome shotgun sequence.
ACCESSION   DAOPBK010000031 DAOPBK010000000
VERSION     DAOPBK010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17982
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3226_length_17982_cov_5.000725"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLA17_03095"
     CDS             complement(<1..835)
                     /locus_tag="PLA17_03095"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPK84428.1"
                     /translation="MAKIKVAINGFGRVGRNAFKIAFEEHPNIEIVGINGRTDAKTLA
                     HLLKYDSTQGRFPGVTHDDQHIIVRGQKIKVTAENNLTAIPWAVTPDIVIESTGIYTT
                     RESPKGGYGDHLKNGAKKVLLTFPAKDTVEAMIVVGVNDEILKPEHTCVSNASCTTNC
                     LAPVAKVLNDTFGIELGFMTTIHSYTNDQRILDRSHKDLRRARSAALSQIPTTTGAAK
                     SVGKIIPELAGKLDGLSVRVPTPTGSLVDFVAILKKTATKEEINAAMQKAAEGPMKGI
                     IE"
     gene            complement(917..1711)
                     /locus_tag="PLA17_03100"
     CDS             complement(917..1711)
                     /locus_tag="PLA17_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84429.1"
                     /translation="MIKRFWHVILFISICGSLAGQGDIDPQQRIFWRNEKSIGPSLNA
                     DGWSLIYRDLRQFKPNQRWFMEGSLEVFKHPKEVKLSNYYFQSPGSFVFGKLNSTWTI
                     GAGAGYQLEIFEKHDLGGVSVSWFGGGGATLLFAKPIYYKIIVLLGNDYYTIEEQKFD
                     LTIHQPLDIMSKASFFKGFDEIKLYPGLYTRTGFNFEYSRNDRITHTVEVGASLHAFT
                     KTIPIMAGDDNKQFFPSIFVGYRIGIILDPVNQRSLFDLLRKQPAE"
     gene            complement(1755..1880)
                     /locus_tag="PLA17_03105"
     CDS             complement(1755..1880)
                     /locus_tag="PLA17_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84430.1"
                     /translation="MKKVGLIAVLALLAVLAFSSCNREACPAYSSADAASTEQEG"
     gene            complement(2134..5436)
                     /locus_tag="PLA17_03110"
     CDS             complement(2134..5436)
                     /locus_tag="PLA17_03110"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84431.1"
                     /translation="MMKKLLTLIFVLLLAGSGSLWAQRKDAKKDAKASESQARPSVPS
                     SFFNAISFRSVGPAWASGRIADLAVNPSNHSEIYAAVASGNIWKSENNGTTWKPIFDK
                     YGAYAIGCIVIDPGNHNVVWAGTGENNHQRALGWGDGVYRSDDAGASWKNMGLKESRQ
                     IGGIAIDPRNSDVVYVAAEGSAWGPGGDRGLYKSTDGGKTWEKILNISENTGVNNVVL
                     DPRNPDVIYCTSEQRRRHVGLKIGGGPESAVYKSTDAGKSFEKIMKGLPGGDIGGMGI
                     AVSPVNPDVLYLIMEAQGDAGGFFRSVNRGASWEKMSSYHSTGQYYNKIICDPKDVNR
                     IFSLDTYTQVTVDGGRTWKNLGNKERHVDDHAMWIDPTNTDHFYIGGDGGIYETYDNG
                     SNFIFKSNLPVTQFYRVAVDNSYPFYYIYGGTQDNNSMGGPSANTRNGVVNDDWFVTS
                     GGDGFWLAIDPEEPDIVYTESQYGNASRYDRRSGERISIKPFPGEGELTYRWNWDTPI
                     LISPHNNKRIYMAANFLFRSEDRGSSWKTISPDLTRNENRDNFKVMDRYWPSDAVAKN
                     RSTSLWGTIVSLSESRLQEDLLYTGSDDGVIAVSEDGGASWRMNKTYPGVPEYTWVSD
                     VLADKHNVNVVYATFNNHKRDDFRPYVLKSTDKGRTWTSISSNLPANGPVHTIEQDHV
                     NPNLLFLGTEFSFFVSFDAGATWTEFNRGLPTIAVRDIAIQERENDLVIATFGRGFYI
                     IDDYTPLQNYSNSITTKAGYIFPVKDAKMFVQTSGFDNQGSMYYVAENPPYGATITYY
                     VNDVPKTARDIRKENEKKLAEKGDFIPQPTARELSLEEREIKPYLIFTITDEEGSVIR
                     KLYKNASKGVGRVTWNFTYAGYQPVRSSGKFNPVAEEGRGRWGGGGISVMPGKYFVSM
                     AIYANGEIKELAGPEPFVCKPLDIVTLPVTNAAAKEKWMKEMAAFAKTAYGAISYVTE
                     LDATVNTIMQTIHQMPDAPASLMKEAAALSDEMNELTYLIRGISVGASTEETPPSPVS
                     LSARLSTMSRSLYSNSGDISGIADQQFTILKKEFPALLERIQKAGRAVDALNAKLDEI
                     KAPWTTGRVPKF"
     gene            5639..6382
                     /locus_tag="PLA17_03115"
     CDS             5639..6382
                     /locus_tag="PLA17_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPK84432.1"
                     /translation="MNSTYFYRRTIRKYAQRDIDNELLERLLMAGTRASTTGNMQVYS
                     IIVTRDQEMKKRLSPAHFNQPMVTEAPVVLTFCADFNRFNLWCRLRDAEPGYDNYLSF
                     MTAAIDALLAAQTICNAAEEEGLGICYLGTATYNADSIIEVLELPRGVVPVATVTLGW
                     PAQVPEQPDRLPLEAVVHYEKYHEFTPELIDSLYREKEARADSRQFIAENNKSTLAQV
                     FTDVRYKKADNEHFSEKFLDVIRRQGFAV"
     gene            complement(6600..7676)
                     /locus_tag="PLA17_03120"
     CDS             complement(6600..7676)
                     /locus_tag="PLA17_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPK84433.1"
                     /translation="MTYDHKYLLDFTTGVFSRVGCNHDDAAAVAAVLVAAELRGHSSH
                     GMIRVREYYELWKSGRVNVSPDVKVVHETPSTAVVDGDRCFGMVAGVRSMRLAIEKAA
                     ATGTGWVAARNSNHFGIAGYYAMMALEHDMVGIAVTNANPLVVPTFSVSRFLGTNPLA
                     VAVPALEEPPFVADFATTPIARGKLAVSEKKGEKVALGYVNDADGNPSDDPGILRRGG
                     GMVTLGGDHQHGSHKGYCMTAVIDIFSALFSGANFGPFVPPSVAYLPVPENQPGKGTG
                     HFFGAMRIDAFRPANEFREAMDHWIRTFRSAKPAKKEQRVLIPGDPEREAEKRFRQEG
                     IALVPAVVDDLKAIAAELGFEFGV"
     gene            complement(7770..11078)
                     /locus_tag="PLA17_03125"
     CDS             complement(7770..11078)
                     /locus_tag="PLA17_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPK84434.1"
                     /translation="MKKNLFAIAIIAVLVLGASGNLSGIDKNNTRMLSQPAASNTHVA
                     FIYAGDLWVMNADGTNPRRLTVDEGIESQPCFSPDGRLIAFSAQYDGNTDVFVIPVEG
                     GVPVRLTWHPGADYARTFTPDGAKVVFSSQRASFTGRYSQLFTVGIEGGYPEQMQIPN
                     AHYVSLSPDGTGIAYTPLSPVHAQWKNYRGGTVSTIWLFTQTDRSVIKVPQPQGGCND
                     THPMWIGDRIYFLSDRNGEFNLFSCSSSGADIRQHTTFTDFPVLNASATTGRIVFEQA
                     GYLHSYDIASGESERLKTAIAADLLELRPRYVQDPGYIRSWHISPTGSRAVLGYRGEI
                     ITLPAEKGDPRNITNTPGSHEKYPAWSPDGKTIAFFSDESGEYTLNLKPQDGKGEVRV
                     YKLPGTGFYAYPVWSPDSKKITFTDNGRNLWLLDLTTSAIRKIDSDEIVFPGPFRKMH
                     GDWSSDSRWIVYTKMLNSFYRVVWLYSVDRQKSFPVTDGLSDASDPVFDPAGDFIYFF
                     ASTDAGPVINWFDLSSADMRMTNSIYLVTLRKEIISPFAKESDEEKGTEAGPDADKPA
                     ATNPEKSSGKGSSKSAAKAPATEKSDLLKVETDGLQERIVSVPLSAGNYSSLGVAGKG
                     EILYIVTNPADRRSMLHKYNVSDRKDEEVMELNSYVVSADRKKMLYTRGSTYGITAAG
                     KKPEPGRGVLNTGAIQVRIDPKEEWSQIFDEAWRINRDFFYDPGMHGADWKAMKEKYS
                     VFLPHLTCRSDLNRVIQWMCSELGVGHHRVGGGERLNDPQTVGGGLLGADFSLENNRY
                     RLKKIYGGLNWNPGLRSPLTEPGLNVTTGEYILAIDGSDVIADENIHRFLENKDGKIV
                     ELTIGPNPDYTGSRVIKVVPIGNELSLRNRDWVEGNLRKVTEATDGKVAYVYVPNTSG
                     QGHEYFKRYFFPQANRQAIIIDERFNGGGMIADYYIDLLQRPLQSYWNMRYGNDLKTP
                     SASIQGPKVMIIDETAGSGGDMLPWMFRRFGVGTLVGKRTWGGLVGTLGFPELMDGGS
                     VTAPNLAIWTEDGFVVENVGVAPDIEVEQWPADVLEGRDPQLEKAIEVALRELEKNPP
                     VTPVRPPYPVRVRK"
     gene            complement(11075..11845)
                     /locus_tag="PLA17_03130"
     CDS             complement(11075..11845)
                     /locus_tag="PLA17_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK84435.1"
                     /translation="MQKTVLITGATSGIGEATAVKFAESSDRLIITGRRKERLDELAD
                     HLRKTYGTEVLTLCFDVRNRHEVEEHLGALAEEWKKIDILINNAGLAVGLSHIDEGDT
                     DDWDRMIDTNVKGLLYITRIVVPLMVARGTGHIFNIASIAGKEVYENGAVYCASKHAV
                     DALSKAMRIDLLKHGIKVTNIAPGMVETEFSEVRFKGDTERAATVYAGLRPLTGDDIA
                     GVIHYCASLPDHVCINDVLITPKAQANATITFRKTGQP"
     gene            11993..12739
                     /gene="ubiE"
                     /locus_tag="PLA17_03135"
     CDS             11993..12739
                     /gene="ubiE"
                     /locus_tag="PLA17_03135"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPK84436.1"
                     /translation="MHDTTRNSGPSAEKVESMFDSISRHYDRLNHLLSFGTDRLWRRR
                     AVSLIGRHIKPEKMLDVAAGTGDLAIEALRLHPEKITGIDISSGMLGEGRRKIEEMGY
                     GDRIELLKGDVSATGFSNGAFDVVMSAFGVRNFENTLAGLTEMYRVVRPGGMIMVLEF
                     SKPEWFPFRQIYGFYFRRILPFIGGRISGDRSAYSYLPDSVMAFPDNEKFLALLEKAG
                     FRRVAQKRLTGGIASIYSGFRPGEEQCNKS"
     gene            12814..13473
                     /locus_tag="PLA17_03140"
     CDS             12814..13473
                     /locus_tag="PLA17_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPK84437.1"
                     /translation="MPLLLVAQKAKPKNDSNYDDRILHFGFSMGVNTMDFIVNNKADS
                     ELKAEVTSLRPGINIQIVTDFRPATYLDLRFLPGVSFGQRNINFYDTNGVKWGETQVI
                     ESSFLEFPLLLKAKGMRLNNSRPYLMTGVNFRYDLAGKKEYDEDKAVHIRLKRPDLYY
                     EVGAGIDFYLPYFKLTVEAKMSTGLFDIMVHEPSPGFEEYVTSIGSLRSQIWSLSFHF
                     E"
     gene            complement(13433..14251)
                     /locus_tag="PLA17_03145"
     CDS             complement(13433..14251)
                     /locus_tag="PLA17_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPK84438.1"
                     /translation="MHRKITRNHVKLILSLQKRKVREDKSLFVIEGDKLVREYILAGN
                     RVMLLAGKPEWIDSESEAVIGGADEVVMVSYEELRRISSLRTPHNVLAVAPMRKVQYS
                     DDSLRGRLTPVLECVQDPGNLGTIMRIAAWFGMTNIVCSPDSVDSYNPKVIQATMGAF
                     MHVNVRYRPLDELLGYAAGEGIPVYGTTTDGTSVYDSELGREGIILFGNESKGISAGL
                     LKHVTSRITIPGPPNPAAGLESLNVSMAAAIICSEFARRGRSIQNGKRETISGI"
     gene            14311..16668
                     /locus_tag="PLA17_03150"
     CDS             14311..16668
                     /locus_tag="PLA17_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPK84439.1"
                     /translation="MIKLRANINITGPKLFLLVIIIFTAVSCNPTKYVPSGELLLSRN
                     ELSVNHGDEPLPATVNRSAMKPYIRQQPNKRFLGMRFHLGLFNLSNITREKWPHGWLR
                     RIGEEPVIYDQVAAERSAGQIQSYLRSKGFFNATVTDTVNVLSGNEAKVVYRVNPGRP
                     YRIADIRYEIQDSILSSLIMIDTVNCTVDRGMIYDVDLLRKERQRLERFIRDIGFYTF
                     STENVFYRIDSSLMNHQMIVNYVVSPGVTFDSRGMQVTTPHRMYRIRDIYLYPEFDPR
                     TALTGGEEYTSTLDTSYFGGIHFVSPPGRTLIRPEVLAQAVYIAPGSLYSVTNTERTQ
                     QHLNDLRNHRLVNVSFIDAGSLSGERRDEGMLDCVIQLTPMQRQSFTVELEGTNTGGN
                     LGGALNLIYQNKSLLKGAEVFSLKLKGAYETLTEDITGFRNTQQYGIEATLRIPKFMT
                     PFPAKESFIRNKDPKTVLTAGYNYQKLPILARSSANISVGYSWNGNKYTRFSMNPLSL
                     NVVRLHYINPDFQAMINDKPYFVNSLRDVRIVGGRYDFVFSDQMILKSRDFWNLRLGL
                     DIAGNLLDVAYRAAKAKPAPDGSYHLFRQPFAQFIKGEIDAAYNYRFNEASSIVYRFL
                     AGVGWPYGNSEVMPFEEQYFGGGSNDIRAWMVRTLGPGSYVQDTSLIQTADMKLEANV
                     EYRFKLFWILEGALFVDAGNIWTVREDADRPGSVFRLRSFADDIAVGTGLGLRFDLKF
                     VLLRADFGLKVRDPRETDGSKWIPFNRPYNRKKDITMVIGIGYPF"
     gene            16776..17882
                     /locus_tag="PLA17_03155"
     CDS             16776..17882
                     /locus_tag="PLA17_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPK84440.1"
                     /translation="MKRFRKSITALFGFDRRERRGTFVLSLIVVVLLLVRLTAMRPGE
                     VPAELPPLPPAEDVIAVGSDRETASPVLFIFDPNTCSYDDLLRLGLSARQARTLVNYR
                     SAGARFRRPDDLRRVYGIDSASAARLIPYIFIEGDPEGKESGGRKEWSGAEQEGRKPG
                     SRKGWSGADQVRKASVASANGYRSDPQRRPSADQAETAEGVPQYNHGDNRAASTALGD
                     EVALDINRCSAGELVALPGIGPVLAERIIKYRNLLGGFVDKWQLAEVYGLDSSVARLA
                     AERLTLTFDEVRPLVLDSASYSDLARHPYLGYETARKITTYRMVSGLPVTLGEMVSSG
                     VITSDQAGRIAPYVRPAPGVTGTDYEFISSKVLK"
BASE COUNT         4137 a         4622 c         4680 g         4543 t
ORIGIN      
        1 attcaataat gcctttcatc ggaccttcag cggccttttg cattgctgcg ttgatctcct
       61 ccttcgttgc ggtcttcttc agaatggcaa caaagtcaac cagtgagccg gtgggcgtcg
      121 gcaccctcac tgaaagacca tcaagcttgc cagccagttc gggtatgatc ttgccgactg
      181 acttggctgc accggtggtg gtcggaatct gtgagagggc agctgacctt gccctgcgga
      241 gatccttgtg cgaacgatca aggatacgct ggtcattggt gtatgagtga atggtagtca
      301 taaagccgag ctcgatacca aaggtgtcat tcaggacctt cgctaccggt gcaaggcagt
      361 tcgtggtgca ggatgcattt gatacgcagg tatgctccgg tttgaggatt tcatcattca
      421 caccaacaac tatcattgcc tcaacggtat ccttcgcggg gaaagtgaga agcacctttt
      481 tcgcaccatt cttcaggtga tcaccgtaac ctcccttggg gctctccctc gttgtgtata
      541 taccggttga ttcaattacg atatcaggtg tgactgccca tggaattgct gtaagattgt
      601 tctctgccgt gactttgatc ttctggcccc ttactatgat atgctgatca tcatgggtta
      661 ctccgggaaa ccgtccctga gtagagtcat atttaaggag atgagcaagt gtcttcgcat
      721 cggtgcgacc attgattcct actatctcaa tgtttggatg ttcttcaaag gcgatcttga
      781 aagcgttgcg accgactctg ccaaacccgt tgattgctac ttttatcttt gccatagttg
      841 taaaataata aggttactaa aataatcgac aaaaatacat aaaatataat aaggagagag
      901 caacaatcag aaataattat tctgcgggct gcttccggag aagatcaaac aggctccgct
      961 ggttgacggg atcgaggatt atgccaatac ggtaacctac aaatatcgaa gggaagaact
     1021 gcttgttgtc atcgccggcc attatgggaa tggtctttgt aaatgcatga agtgaggcgc
     1081 caacctccac ggtgtgggtt atccggtcat tcctgctgta ttcaaaattg aacccggtgc
     1141 gcgtataaag ccccgggtag agtttaatct catcaaaacc cttgaagaag gaggctttgc
     1201 tcatgatatc gagcggctgg tgaatggtca ggtcaaattt ctgctcttct attgtatagt
     1261 agtcgttccc gagcagaacg atgatcttgt aatagattgg tttggcgaag agcagggttg
     1321 ccccgccacc tccaaaccag ctgaccgaaa cccctcccag gtcatgtttc tcaaatatct
     1381 cgagctggta accggcccct gccccgatgg tccatgtgct gttcagcttg ccgaacacaa
     1441 aggaacccgg actctggaaa tagtaatttg agagctttac ctccttgggg tgcttgaaga
     1501 cttcgaggct cccttccata aaccagcgct gattgggctt gaactgacgc aggtcacggt
     1561 aaatgagtga ccagccgtca gcattgagtg acgggccgat agatttctca ttgcgccaga
     1621 aaatcctttg ctgagggtca atatctccct gacccgcgag agacccgcag atagagataa
     1681 aaagtatgac gtgccaaaac ctcttaatca ttattacatt aatgttaaga ctggcagcgg
     1741 gggagaagct atgatcagcc ttcctgttcc gttgaagcag catcggcact gctgtatgcc
     1801 ggacatgctt cacgattgca tgaactgaat gccagtactg caagcagagc gaggacggct
     1861 atcagaccaa cttttttcat aattcctgcc tttaagtctg agacaaaagt aactttattt
     1921 tttaaacaag atttgtgcca tattattatt tatgcccgct ccttttttaa acaaacaatg
     1981 ctgtcagtga actgctgact gcaggccaca gagtgaagaa accccggtat gcctgatggc
     2041 tgccggggtt tcaaaaagag agagggtgcc ccatttgtat tatgagacac cctcctgatt
     2101 atttgcaaat ctcaacgtgc aatgttaaat cccttagaat ttgggcactc ttccggttgt
     2161 ccatggagcc tttatctcat ccagtttcgc gttgagcgca tcaacagccc tgcctgcctt
     2221 ctggattctc tcaagcaacg ccggaaactc cttcttcagg atagtgaact gctggtcagc
     2281 aatgccggag atatcaccgg agttgctata gagcgatctt gacatggttg acagcctcgc
     2341 agagagcgat acaggtgacg gaggcgtctc ctcggtgctg gcgccgacag agatacccct
     2401 gatgagatat gttagctcat tcatctcatc actcagcgct gcagcttcct tcatcagtga
     2461 tgcaggtgca tccggcatct gatggatggt ctgcataatg gtgttaaccg ttgcgtcaag
     2521 ctcagtgaca tatgagattg cgccgtaagc agttttcgca aaggcagcca tctccttcat
     2581 ccatttttct ttggctgctg cattggtgac cggcagggtc actatgtcaa gcggtttgca
     2641 cacgaagggc tcgggaccgg ccagttcttt aatctcgcca ttggcgtaga tagccattga
     2701 aacgaaatat ttgcccggca tgaccgagat gcctccgccg ccccagcggc cacggccttc
     2761 ttcagcaacg ggattgaact tcccggagga cctgacaggc tgataaccgg cataggtaaa
     2821 gttccatgtc acacggccca ctcccttcga agcattttta taaagcttgc ggattaccga
     2881 accctcctcg tcagtaatgg tgaagataag atatggcttt atctctcgct cctcaaggct
     2941 gagctccctt gctgtaggct gcggaatgaa atcacccttt tcagcaagtt tcttttcatt
     3001 ttccttcctg atatcacggg cggtcttcgg aacatcgttc acataatagg tgatggtagc
     3061 tccatagggt ggattctccg ccacataata cattgatccc tggttgtcaa acccactggt
     3121 ctgtacgaac attttggcat ccttgacggg gaagatatat ccggcttttg tagtaatgct
     3181 gttggagtaa ttctgcagcg gtgtgtagtc atctatgata taaaatcccc ttccgaatgt
     3241 tgcaatcacc aggtcgttct cacgctcctg gatggcaatg tcacgcactg cgattgtcgg
     3301 aaggcccctg ttgaactcgg tccatgtggc gcctgcgtca aatgaaacga agaagctgaa
     3361 ctcagttccc aggaagagca ggttgggatt gacatgatcc tgctctattg tatgaaccgg
     3421 tccgtttgcc ggcaggttgg agctgatcga tgtccatgtg cgtcccttgt cagtgctctt
     3481 cagtacatac ggcctgaagt cgtcacgctt gtgattgttg aatgtcgcat agaccacgtt
     3541 tacattgtgc ttgtcagcca gcacgtcact cacccaggta tattccggca cgccgggata
     3601 ggttttattc atcctccagc tggcgccgcc gtcttccgag actgcaatta ctccgtcatc
     3661 acttccggta tagagcagat cctcctgcag gcgcgattcg gaaagggaga ctattgttcc
     3721 ccagagtgag gttgacctgt ttttcgcgac agcgtctgac ggccagtacc tgtccatcac
     3781 cttgaagttg tcgcggttct cgtttcttgt caggtcaggc gatattgtct tccagctgct
     3841 tccgcggtct tcactgcgga acaggaagtt ggcagccatg tagatccttt tgttgttgtg
     3901 cggactaata aggattggag tgtcccagtt ccagcggtag gtgagttcac cctcaccggg
     3961 gaagggcttg atgcttattc tctcgccgct tctgcggtca tagcgggatg cattgccgta
     4021 ctgtgattcg gtatagacga tgtccggttc ttccggatcg attgccagcc agaatccgtc
     4081 acctcccgag gtaacgaacc agtcgtcatt gacaacgccg tttctggtgt tggccgaggg
     4141 gcctcccatg gagttgttgt cctgcgtacc gccgtagatg taatagaagg ggtatgaatt
     4201 gtcaacagcc acccggtaga actgggtcac cgggaggttt gacttgaaga tgaaattgct
     4261 tccgttgtca taagtctcgt agataccgcc gtcgccaccg atgtaaaagt ggtcagtgtt
     4321 ggtcggatca atccacatgg catggtcgtc aacatggcgt tccttgttgc ctaaattctt
     4381 ccatgtgcgg ccgccgtcaa cagtcacctg ggtgtaggtg tcaagactga atatcctgtt
     4441 tacatccttc gggtcacata tgatcttgtt gtagtactgc ccggttgaat gataggagct
     4501 catcttctcc catgaagcgc ccctgttgac cgacctgaag aagccgccgg catcaccctg
     4561 tgcttccatt atgagataga gaacgtcagg gttaaccggc gagacggcta tgcccatgcc
     4621 gccaatgtct cctcccggaa gccctttcat tatcttctca aatgattttc cggcgtcggt
     4681 tgacttgtat actgctgatt ccggtcctcc gccgattttc agtccgacgt gcctgcggcg
     4741 ctgttcgctt gtgcaataga tgacatcagg attgcgcgga tcaagcacca cgttgttgac
     4801 gccggtgttc tcactgatgt tgagaatctt ttcccatgtt tttccgccgt cagttgattt
     4861 atagagtccg cggtcaccgc ccgggcccca tgcagaccct tccgctgcta cataaaccac
     4921 atcgctgttg cgggggtcaa tggctattcc gcctatctgg cgcgactcct tcaggcccat
     4981 attcttccag ctggcacctg cgtcatcaga gcggtaaacg ccgtcacccc atcccagtgc
     5041 acgctgatga ttgttttctc cggtgccggc ccagaccacg ttgtggttgc ccgggtcaat
     5101 gacgatacag ccgatggcat atgcaccgta cttgtcaaaa atcggtttcc aggtggtgcc
     5161 gttgttctca ctcttccaga tgttgcccga ggcaacagct gcgtagatct ccgagtgatt
     5221 tgagggatta accgcgaggt ctgctatacg gcctgaagcc catgccggtc ctacagacct
     5281 gaaagagatg gcattaaaga atgatgaggg tacggagggc cttgcctggc tctccgatgc
     5341 cttcgcatcc ttcttcgcat ctttcctttg tgcccacagg ctgcctgagc ctgccagcaa
     5401 caacacaaaa atcagtgtca gaagtttttt catcatgata taattttgat tttaaaggta
     5461 tgacggaacc cgcatgactg aaaattacat aaacagttgt atgtgtattt gatacatgag
     5521 ggtttcaaaa gtggtttcct ggttggtttg ttactcttgc taagttaaga taaggaatta
     5581 tgtcatctga ctttttaacg ttatttaatt attttgctgc agattaaaat aagtcgtgat
     5641 gaacagtaca tatttttacc ggagaacgat aagaaaatac gcacaacgag atattgacaa
     5701 tgaactcctt gagcggctgc tgatggcagg aacaagagcg tctaccacgg gtaacatgca
     5761 ggtatacagt attatagtga ccagggatca ggagatgaaa aaacgactct caccggccca
     5821 tttcaaccag cccatggtaa cggaggcgcc ggttgtgctt accttctgtg ccgacttcaa
     5881 caggttcaac ctgtggtgca ggctgcgtga tgccgagccc ggctatgaca attacctgtc
     5941 gtttatgact gcggcaattg atgcacttct ggctgcccag acaatatgca atgctgctga
     6001 agaggagggg ctgggcatct gctatctcgg aaccgccacc tataatgcag acagtatcat
     6061 tgaggtgctg gaacttcccc ggggcgtggt acctgttgca actgtcaccc tgggctggcc
     6121 ggcacaggtg ccggagcagc ctgacaggct acccctggaa gcggtagtgc attatgaaaa
     6181 ataccatgaa ttcaccccgg agctgatcga cagcctgtac agggagaagg aggcacgtgc
     6241 tgactcccgg cagttcattg ccgaaaacaa caagagcacc ctggcacagg tctttaccga
     6301 cgtccgttac aaaaaggctg ataacgagca cttttcagaa aaatttctgg atgtgatcag
     6361 gagacagggg tttgctgtct agaatccagc agccggccgg gggggcatca gcattcggcg
     6421 ggctttgctt tccggaagcc gccaactggc cggaccttac gctccggaag cggccagaat
     6481 gagcctgccc tgcatgagga agatcagttc catgcccgaa tctggatatc tgtcagaagg
     6541 aacgcatctg tcagtgtatg aagaggatca cttcagttct ttgagtaccg gggcaatgac
     6601 tataccccga actcaaatcc cagctcggcg gcgatggcct tcaggtcatc aaccacggcc
     6661 ggcacaagcg caatcccttc ctggcggaat cttttctccg cttctctttc cgggtcaccg
     6721 gggatcagca cacgctgttc cttctttgcc ggtttggcgc tgcggaatgt tctgatccag
     6781 tgatccattg cctccctgaa ctcatttgcg ggcctgaagg cgtcaattct catggcgccg
     6841 aagaagtgtc cggtaccttt gcccggctgg ttctccggca ccggcaggta agccacgctg
     6901 ggtggcacga acgggccgaa gttggctccg ctgaagagag cgctgaatat atcaatcaca
     6961 gcggtcatgc agtacccctt gtggctgccg tgctggtgat cgccccccag ggtgaccatg
     7021 ccaccgccgc ggcgcagtat gcccgggtca tcggaaggat tgccatcggc atcgttaaca
     7081 tatcccaagg ctaccttctc gcccttcttc tcggatactg ccagcttgcc ccgtgcgatg
     7141 ggagtggtgg cgaagtcagc cacgaagggt ggctcctcaa gtgcgggaac tgccactgca
     7201 agagggttgg tacccaggaa gcggctcacc gagaaggtgg gaaccacaag cggattggcg
     7261 ttggtgacgg caatgcccac catatcatgt tcgagagcca tcattgcata gtaaccggct
     7321 atgccgaagt ggtttgagtt acgggctgcc acccagcccg tgcctgtggc agccgccttc
     7381 tcaatggcca gacgcatcga cctgaccccc gcaaccatgc cgaagcacct gtcaccgtcg
     7441 acaacggcgg tggagggggt ctcatgaacc accttcacgt cagggctgac gttaacccgc
     7501 ccgctcttcc acagctcata gtactcgcgg acccttatca tgccgtgcga tgagtggccg
     7561 cgcagctccg ccgccaccag cacggcagca acggcagcag cgtcatcatg gttgcaaccc
     7621 acacgggaga aaactccggt ggtgaaatca agaaggtatt tgtggtcgta tgtcatgata
     7681 tacagttttt tatgctttga ctattaatta ttaactggcc ggtttgtttt gccacgtgac
     7741 gcatcttcat gcgtccggca tccggctgac tatttcctga ccctgaccgg atatggcggt
     7801 ctgacgggtg tgaccggcgg gttcttctcc agctccctga gagccacctc aatggctttc
     7861 tcaagctgcg gatccctgcc ttcaagcaca tcggcaggcc actgttcaac ttcaatgtca
     7921 ggggccacac ctacattttc aaccacaaat ccatcttcgg tccagatagc cagattgggc
     7981 gctgtaaccg atccgccatc catcagttcg ggaaaaccca gtgttcctac cagtccgccc
     8041 caggtacgct tgcctaccag ggtgccgaca ccaaacctgc ggaacatcca gggaagcatg
     8101 tcaccgcctg aaccggccgt ctcatctatg atcatcacct tcggcccctg aattgaggcg
     8161 ctcggggtct tgagatcatt gccgtagcgc atgttccagt atgactgcaa cgggcgctga
     8221 agcagatcaa tgtagtagtc ggcaatcatc cctccgccat tgaaccgttc gtcaatgata
     8281 atagcctgcc tgttggcctg cgggaaaaag tacctcttga agtactcatg accctgcccc
     8341 gaggtgtttg gcacatatac ataagccacc ttaccatcgg ttgcctcggt gacctttctc
     8401 aggttaccct caacccagtc acggttcctc agactgagct cgttgccgat gggtacaacc
     8461 tttatcacac gtgaaccagt gtaatcagga ttcggcccaa ttgtaagctc aacgattttt
     8521 ccatctttat tctcaaggaa cctgtggatg ttttcatctg cgatgacgtc actgccgtca
     8581 atggcaagaa tatattcccc ggtcgtgacg ttcagtcccg gttcggtaag aggtgacctg
     8641 agaccggggt tccagttaag tccgccgtag atttttttca gacggtacct gttgttttcc
     8701 agcgagaagt ctgcgccgag gaggccgcct ccgaccgtct gcgggtcatt cagcctctca
     8761 cctccgccaa ccctgtggtg gccgacgcca agctcactgc acatccactg gatgaccctg
     8821 ttgaggtcac tcctgcaggt caggtgaggc aggaacacgg aatacttctc tttcattgcc
     8881 ttccagtcgg ccccgtgcat gcccgggtca tagaaaaagt cacggttgat cctccatgcc
     8941 tcatcaaata tctgagacca ctcttctttc ggatctattc tgacctggat tgccccggta
     9001 ttcagtacac ccctgcctgg ttccggtttc ttgcctgcag ccgtgatccc gtaagtcgaa
     9061 cccctggtat agagcatctt cttcctgtca gctgacacaa catagctgtt aagttccatc
     9121 acctcctcat ctttcctgtc agagacattg tatttatgaa gcatcgacct gcggtctgca
     9181 gggttggtaa caatgtatag tatttcacct tttcccgcta ccccaagtga actgtaattg
     9241 ccggcagaca gcggtacgct tacgattctt tcctgcagcc cgtctgtctc caccttcagc
     9301 agatcactct tttccgtggc aggtgccttt gctgcgctct ttgatgaacc cttgccggag
     9361 ctcttttcag ggttagtggc tgcaggttta tccgcgtcgg gtccggcttc tgttcccttt
     9421 tcttcatcac tctcctttgc aaagggtgag atgatctctt tacggagggt gacaagatag
     9481 attgagtttg tcattctcat gtctgcgctc gaaaggtcga accagttgat gaccgggccg
     9541 gcgtcagtgg atgcaaagaa atagataaaa tcacctgccg ggtcaaagac gggatcggag
     9601 gcatcgctca gcccgtcagt gacgggaaat gatttctgcc ggtcaacgga atagagccag
     9661 acaaccctgt aaaaactgtt cagcatcttc gtgtacacga tccacctgga gtctgatgac
     9721 cagtcgccgt gcatctttct gaacggcccc gggaagacaa tctcgtcaga atcgattttc
     9781 cttattgctg aggttgtcag gtcaagcagc cataggttcc ttccgttatc cgtaaaggtt
     9841 atctttttgc tgtcaggtga ccataccggg taagcataga atcctgttcc cggcagtttg
     9901 tacaccctga cctcaccctt tccgtcctgc ggtttcaggt tcagggtata ttcaccggat
     9961 tcatccgaga agaatgcaat tgtttttccg tcaggtgacc atgccggata tttttcatga
    10021 ctgccgggtg tattggtgat attcctcggg tctccctttt cagcaggaag ggtgattatt
    10081 tctcccctgt agcccagcac agcgcggctt cccgtcgggg agatgtgcca gctccggatg
    10141 tatcccgggt cctgaacata acgcgggcgc agttcaagca ggtcagcggc tatggctgtc
    10201 ttcagcctct ccgattcgcc actggcgatg tcatatgaat gaaggtatcc ggcctgttca
    10261 aagacaatcc tgcccgttgt ggctgaggcg ttaagcacgg ggaagtcagt gaatgtggtg
    10321 tgctgtctga tgtcagcgcc actgctgctg catgaaaaga ggttgaactc tccgttacgg
    10381 tcactcagga aatatatcct gtcgccgatc cacatgggat gggtatcatt acatcctccc
    10441 tgtggctgcg gcacctttat cactgacctg tcggtctgtg tgaagagcca gatggttgaa
    10501 acggttcctc cgcgatagtt tttccactgg gcatgtaccg gggagagcgg ggtgtaggct
    10561 attcccgttc catcgggaga cagggagaca tagtgagcat ttggtatctg catctgttcc
    10621 gggtatcccc cttcaattcc caccgtgaag agctgtgaat agcggccggt gaatgacgcc
    10681 ctctgcgagg agaagaccac cttcgccccg tcaggggtaa aggttcgtgc ataatcagct
    10741 cccggatgcc aggtgagtct taccggcaca cctccctcca ccgggatgac aaagacatcg
    10801 gtgttcccgt catactgggc gctgaatgct atcagcctgc cgtcagggga gaaacagggt
    10861 tggctttcaa ttccttcatc taccgtaaga cggcggggat tggttccatc ggcattcatc
    10921 acccagagat ccccggcgta gataaatgcc acgtgtgtat tactggctgc gggctgtgac
    10981 agcatccgcg tgttattttt gtcaattcca ctgagattgc ctgatgctcc cagaacgaga
    11041 actgcaataa ttgcaattgc aaagagattt tttttcatgg ctgtccggtt tttctgaaag
    11101 tgattgttgc gtttgcctgt gccttgggtg ttatgagcac gtcattgatg cagacatgat
    11161 cgggaaggct ggcgcagtaa tggataaccc ctgcaatgtc atcgccggtg agcggcctga
    11221 ggccggcata aacggttgcc gccctttcag tgtcaccctt gaaccgtact tccgagaact
    11281 cggtctcaac cattcccggt gctatgtttg tcacctttat gccatgcttc agaaggtcaa
    11341 tccgcatagc ctttgaaaga gcatccacgg cgtgcttgga ggcacagtaa acggcgccgt
    11401 tctcgtacac ctccttgcct gcaattgatg cgatattgaa aatgtgaccc gtgccccttg
    11461 ccaccatcag cggcacaact atccgggtaa tgtagagaag ccccttcaca ttggtgtcaa
    11521 tcatcctgtc ccagtcatcg gtgtctccct cgtcaatatg actcagtccc actgccagtc
    11581 cggcattatt gatgagaata tcaatcttct tccactcctc tgcaagggcc ccgagatgct
    11641 cttccacttc gtgcctgttc ctcacatcga agcatagggt aagcacttca gtgccataag
    11701 ttttccggag gtgatcagcc aattcatcca gtctctcttt tcttcttcct gtaatgatta
    11761 gtctgtcaga tgattctgcg aacttcacgg ctgtagcttc cccgatgcct gaagttgcgc
    11821 ctgtaataag tactgttttc tgcataacat aactttttca gatgggtaaa tgtaaaaata
    11881 ttaagggtat ctttgccgcc tgtcaggaaa ttgatggttt aatctatcat aaggactcat
    11941 ggatgtgtgg cagtgatgca agcaaaccgc cggccgaaaa aaatctgtag tgatgcatga
    12001 cacaacccgt aacagcggac cttctgcaga aaaggttgag agtatgtttg attcaatctc
    12061 aaggcattat gaccggctta accatcttct ttcctttggc actgacaggt tatggaggag
    12121 gagggccgtg tcactcatcg gcaggcatat taaaccggag aaaatgcttg atgttgccgc
    12181 gggtacgggt gatctggcca tcgaggcgct caggctgcat cccgaaaaga tcaccgggat
    12241 tgacatcagc tccgggatgc tgggggaagg ccgccggaag attgaggaga tgggttatgg
    12301 agacagaatt gagctgctga agggagatgt ctctgcaaca ggattcagca atggtgcttt
    12361 tgatgttgtg atgtcagcgt tcggggtgcg caattttgag aacaccctgg ccggactgac
    12421 cgagatgtac cgggtggtca ggcccggcgg aatgataatg gtgctggaat tctcaaaacc
    12481 ggaatggttc cccttcaggc agatatacgg gttttatttc aggaggattc tgccattcat
    12541 aggaggaagg atttcgggtg acaggtcagc ctattcctat ctgcctgatt cagtgatggc
    12601 atttccggat aatgaaaaat ttctggcact gcttgagaaa gccggattcc gccgtgttgc
    12661 acagaagagg ctgacaggag gcattgccag catatattcg ggattcagac ccggtgaaga
    12721 gcaatgcaat aaaagctaag ttttccgttt tattattcag gaccattcag aagagtgtgt
    12781 aaaagaactg ccatatttat ccttctgctt ctgatgccat tattgctggt tgcccagaag
    12841 gcgaagccaa aaaatgactc gaattatgac gacaggattc tccatttcgg cttcagcatg
    12901 ggtgtcaaca ctatggattt catagtgaac aataaggcgg actctgaact gaaggcagag
    12961 gtcaccagcc tgcggccggg tatcaatatc cagatagtaa ctgacttcag accagcaaca
    13021 tatcttgacc tcaggtttct gccgggagta tccttcgggc agcgaaacat caatttctat
    13081 gacacgaacg gtgtaaagtg gggagagaca caggttatcg aatcttcgtt cctggaattt
    13141 cctcttttgc tgaaggcaaa ggggatgagg ctgaacaatt caagacccta cctcatgacc
    13201 ggggttaatt tccgttatga tctggcgggg aaaaaggagt atgatgagga taaggctgtt
    13261 catatcaggc tgaaaaggcc cgatctgtat tatgaggttg gtgcgggcat agatttttat
    13321 cttccctatt tcaaactgac cgttgaggca aagatgtcga caggtctctt tgatatcatg
    13381 gttcatgaac cttctcctgg cttcgaagag tatgtcacct ctatcggatc actcagatcc
    13441 cagatatggt ctctctcttt ccattttgaa tagacctgcc tcgtctggca aactcagaac
    13501 agattatggc agctgccatg ctgacgttca gtgattccag cccggcggca ggatttggcg
    13561 ggccggggat ggtgatacgg ctggtgacat gtttcagcag cccggcggaa atcccttttg
    13621 attcgttgcc gaagaggatg atgccctccc tgccgagctc actgtcatag accgatgtac
    13681 cgtcagtggt ggttccgtaa acggggatgc cctcgccggc agcgtagcca agaagctcat
    13741 caagcggcct gtaccggacg ttcacatgca tgaaggcgcc cattgtggcc tggattacct
    13801 tgggattgta agagtcaacc gaatcgggag aacagacgat gttggtcatt ccgaaccacg
    13861 cggcaattcg catgatggtc ccgaggttgc ccggatcctg aacgcattcc agtaccggtg
    13921 taagcctgcc ccggagggag tcatcactgt actgcacctt cctcatgggg gctacggcca
    13981 gcacgttgtg cggggtacgg agcgagctta tccgcctcag ctcctcgtag ctcaccatga
    14041 caacttcatc ggccccgccg ataactgctt cagattcact atcaatccat tcaggcttgc
    14101 ctgccagaag catgaccctg ttgcctgcaa gaatatactc cctgaccagc ttgtcgccct
    14161 caatgacaaa gagtgattta tcctccctta cctttcgttt ctgaagagag agtatcagtt
    14221 ttacatgatt gcgggtgatc ttcctgtgca taattaacta ataatagagt ttataatgat
    14281 atatttgcgt gcaccactct tcccgggcaa gtgatcaaac tgagagcaaa tataaatatt
    14341 acgggaccca aactctttct gcttgtcatc attattttca cggctgtatc gtgcaatccc
    14401 acaaaatatg tcccttcggg cgaattgctc ctcagcagga atgaactttc cgtcaatcat
    14461 ggcgatgaac cgttgcccgc gacggtgaac cggagcgcaa tgaagccata tatcaggcag
    14521 cagccaaaca agaggttttt agggatgagg ttccacctcg gactattcaa tctctccaac
    14581 atcacccgtg aaaaatggcc ccatggctgg ctcaggcgta tcggagagga accggtgata
    14641 tatgatcagg tcgctgcgga gaggtctgcc gggcagattc agagctacct ccggtcaaaa
    14701 ggctttttca atgccactgt aactgatacg gtcaatgtct tgtccggaaa cgaggcgaag
    14761 gttgtttacc gggtcaaccc gggaagacct tatagaattg ctgacatcag gtatgagata
    14821 caggattcca ttctcagtag cctgatcatg attgacacgg taaactgcac cgtggacagg
    14881 gggatgatat atgatgttga cctcctcagg aaggagcgcc agagactgga gcggtttatc
    14941 cgcgacatag gcttctatac tttttccacg gagaatgtct tttaccgcat tgacagctcc
    15001 ctcatgaacc atcagatgat tgtcaattat gtcgtctccc cgggggtgac ctttgacagc
    15061 cgtggtatgc aggtgacaac tcctcacagg atgtaccgga tcagggatat ctatctctat
    15121 cctgagtttg atccccggac agcgctgacg ggtggagagg agtataccag cacccttgat
    15181 acctcatatt tcggaggaat ccattttgtg tcacctcccg gccgcaccct catcaggccc
    15241 gaggttctcg cacaggcagt atatattgca ccggggtcac tctatagtgt caccaatacg
    15301 gaaaggacac agcaacacct caacgatctc aggaaccaca ggctggtgaa tgtctcgttc
    15361 atagatgcag gaagcctttc aggcgaaagg agggatgagg ggatgctcga ctgtgtcatt
    15421 cagctcaccc cgatgcagcg gcaatcattc accgtggaac tggagggcac caatacaggc
    15481 ggaaaccttg gcggagccct taaccttatc tatcagaaca agagcctgtt aaagggcgct
    15541 gaagtattca gcctgaagct gaagggtgca tacgaaaccc tcaccgaaga tatcaccggt
    15601 ttcaggaaca cccagcaata tggcattgag gctaccctga ggatacccaa gttcatgacc
    15661 cccttccctg caaaggaatc gtttatcagg aacaaggatc cgaaaacggt tcttacggcc
    15721 gggtacaact accagaagct gccgatactt gcccgctcgt ctgcaaatat atcggttggt
    15781 tattcatgga acggtaacaa gtacaccagg ttcagtatga acccgctctc gctgaacgtg
    15841 gtgaggcttc attacatcaa tcctgatttc caggcgatga ttaacgataa gccatacttt
    15901 gttaattcgc tgcgtgacgt caggatcgtg ggcggacggt atgacttcgt gttcagcgac
    15961 cagatgattc tgaaatcacg tgacttctgg aacctccgtc tcgggctgga tattgccggt
    16021 aacctgcttg atgtcgccta cagggcggct aaggcaaagc cggctcctga cggaagctac
    16081 catctgttcc gtcagccgtt tgcccagttc atcaagggag agattgatgc agcctacaac
    16141 tacaggttca atgaagccag cagcatcgtt tacaggttcc tggccggggt gggatggccc
    16201 tatggcaact cggaggtgat gccttttgag gaacaatact tcggcggcgg atcaaatgat
    16261 atcagggcgt ggatggtcag aacgctcgga ccaggatcgt atgtgcagga tacatcattg
    16321 atccagacag ctgacatgaa gctcgaagcc aacgtcgaat acaggttcaa gctcttctgg
    16381 atactggagg gggctctctt cgtagacgcc ggtaatatat ggacagtcag ggaggacgcg
    16441 gacagacccg gaagcgtttt ccgtctcagg tcattcgccg atgacattgc cgtgggtacc
    16501 ggccttggcc tgaggtttga cctcaagttc gtcctgctgc gtgccgactt cggactgaag
    16561 gtacgtgacc cacgggagac ggatggctcg aaatggatac ccttcaatcg cccctataac
    16621 cgcaagaagg acattacgat ggtgataggg ataggttatc ccttttagtc ggcagttaat
    16681 caatgaatta catactgtag actgcaggcc tgacacctcc ccttgccttg tattccgatt
    16741 tttcatatct ttcatgaggt cttgctgcat cgtttatgaa gcgattcagg aaaagtatta
    16801 cggcactgtt cggttttgac cgcagggaga ggcgcggaac gtttgtactg tcactgattg
    16861 ttgtggtact cctgcttgtg cgccttacag ccatgcggcc cggggaggtg ccggcagagc
    16921 ttcctccgct gccaccggca gaggatgtca tcgcggtcgg cagtgacagg gaaactgcct
    16981 ctccggtgct cttcatattt gatcccaaca catgcagcta tgatgacctt ctgagactcg
    17041 gcctgtcggc caggcaggca cgcactcttg taaactaccg gagcgccggg gcgaggttcc
    17101 gccgcccgga tgacctgcgg cgggtatatg gaatcgattc agcctctgcc gccaggctga
    17161 taccgtatat atttatagag ggagacccgg aggggaagga gtcaggtggc aggaaagaat
    17221 ggtccggagc ggaacaggag gggagaaagc cagggagccg gaaagggtgg tccggagcag
    17281 accaggtgag gaaagcatca gtggcatcgg caaacgggta caggagtgac ccgcagcgaa
    17341 ggccatcggc agatcaggca gaaacagcag agggagttcc gcagtacaac catggagata
    17401 accgggcagc cagcacagca ttgggtgatg aggttgcgct tgacattaac cggtgcagtg
    17461 ccggtgagct ggtcgccctt cccggcatcg ggccggtact ggcagagagg ataataaaat
    17521 accggaacct tcttggcggg tttgttgaca aatggcaact ggcagaggta tacggccttg
    17581 acagctcggt agcccggctg gcagcggagc ggctcaccct gacatttgat gaagtaaggc
    17641 cgctggttct tgacagcgca agctacagtg atcttgcaag gcatccctat ctggggtatg
    17701 agactgcaag gaagattaca acctacagga tggtatccgg gctgccggtc acactcgggg
    17761 aaatggtcag cagtggtgtt atcacttcgg atcaggccgg gcggatagcc ccttatgtga
    17821 gacctgcccc gggcgttaca ggaaccgatt atgaatttat ttcatcaaaa gttttgaaat
    17881 aattttctga gatactaatt ttgcagcctg ttttaaaatc aagttcaaat atgattattg
    17941 taccggtaaa agaaggcgag aacatagaca ggactctcaa ga
//
LOCUS       DAOPBK010000032        17866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3248_length_17866_cov_4.440177, whole genome shotgun sequence.
ACCESSION   DAOPBK010000032 DAOPBK010000000
VERSION     DAOPBK010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17866
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_17866_cov_4.440177"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLA17_03160"
     CDS             <1..202
                     /locus_tag="PLA17_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84441.1"
                     /translation="RTEEGATLQCVRTEEGATLHCVRMEEGATLYCVRMEETENNYVE
                     YYGELWKNLMTAAESCLTATTR"
     gene            148..783
                     /gene="folE"
                     /locus_tag="PLA17_03165"
     CDS             148..783
                     /gene="folE"
                     /locus_tag="PLA17_03165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPK84442.1"
                     /translation="MEKLNDGSGKLSDGYNKIEKWNSEVIEEIAGYYYKILRLLGENP
                     DREGLLKTPERVAKAFSFLTRGYYSEPEEILRSAIFKEEYRQMVIVKDIDIYSLCEHH
                     MLPFHGKAHVAYIPDGYITGLSKIARVVETYAARLQVQERLTTQIRNCIQENLKPLGV
                     AVVIEAQHMCMQIRGVQKQNSVTTTSAFTGCFLSNMNTREEFMHLIANKLH"
     gene            817..1701
                     /gene="fabD"
                     /locus_tag="PLA17_03170"
     CDS             817..1701
                     /gene="fabD"
                     /locus_tag="PLA17_03170"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPK84443.1"
                     /translation="MKAYVFPGQGAQYSGMGKDLYEKFPVAHDMFEKANALLGFRITD
                     IMFSGTDEELRQTRVTQPAIFLHSVILTAVAGSSFKPAMVAGHSLGEFSALVAAGALS
                     FEEGLALVARRASAMQKACNINPSTMAAVLGMDDAVVEEVCAALNGIVVPANYNSPGQ
                     IVISGTMEGVSAATEELKKRGAKRIVPLSVSGGFHSPSMEPARQDLEEAIRKARFSRP
                     LCPVYQNVTGMPVTEPDMIMVNLIAQLTSPVRWTQTIQTMIADGVTEFIEAGPGNVLQ
                     GLIRKINPNVAASSIDPM"
     gene            complement(1823..2845)
                     /locus_tag="PLA17_03175"
     CDS             complement(1823..2845)
                     /locus_tag="PLA17_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPK84444.1"
                     /translation="MAETNNNKYAAKDFKSDQEVRWCPGCGDHAILAALQKTLAELDI
                     PKEKYVFVSGIGCSSRLPYYMNTYGFHGIHGRAAAIATGIKIANPDLMVWEMTGDGDA
                     LAIGGNHYIHAIRRNIDIKIVLFNNEIYGLTKGQYSPTSKKGLITKTSPYGTIEEPFK
                     VGELTIGANGRFFARTVDYNLQLSSEVYKEAAKFHGTAVVEVVQNCVIFNDGTHDAFT
                     SKENRDENTIILRHGEKMIFGKNRDKGIILKDLKLKVVKIGEDGITEKDILTHDAHEP
                     NPGIHYRLAGMSLPEYPVALGVIRAISTKTYDAAMMEQHERVQARSKIRTVDELLKSG
                     ATWEVK"
     gene            complement(2838..4691)
                     /locus_tag="PLA17_03180"
     CDS             complement(2838..4691)
                     /locus_tag="PLA17_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPK84445.1"
                     /translation="MGKRSKVIEKDAVVIRFSGDSGDGMQLTGTLFSNTSALFGHDIS
                     TFPDYPAEIRAPQGTVGGVSGFQVHIGHKLINTPGDFTDILVAMNPAAIRANVRWLKK
                     GSTIIYDSDNFTEKSIQRAGYKADPFAEDKLDSMNLIAAPITTLVKEALKDMDLDPKT
                     VLRTKNMFALGLIYWLFDSRLKYTEEFIEEKFGKKPLVAEANNKVLRAGYYYGETIEA
                     LTPSYRVNRAPIKKGTYRNINGNQATAWGVLAAAEKAGLKLFIGSYPITPATGILEEL
                     ALRKDMGVKSLQAEDEIAGICTSIGASFAGRMAVTSTSGPGLALKSEALNLAVMTELP
                     LLVIDVQRGGPSTGLPTKTEQADLWQALYGRNGESPIPVIAASTPSDCFRFGFEAAKI
                     AIEHMTPVILLTDGYLANGSEPWRIPSTKDLPSITVPYAVRSDKEFLAYERDPKTLVR
                     KWAIPGTPGLEHRVGGLEKTIKGSVSYIPENHEYMVKTREEKVERIVNELPNLEVLGK
                     KTGDLLVIGWGSTYGHITSAVTELQKEGYAVSAAIFNYIRPLPKNTAKVFAGFDKLLV
                     CELNTGQFANYLRIKHQKYTYNQYNKVQGLPFTITEIKDHCVKLLKGKKNG"
     gene            complement(4894..5700)
                     /gene="murI"
                     /locus_tag="PLA17_03185"
     CDS             complement(4894..5700)
                     /gene="murI"
                     /locus_tag="PLA17_03185"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPK84446.1"
                     /translation="METGNRPIGVFDSGVGGLTVAQAIRQILPGEQIVYFGDTAHLPY
                     GDKSEEAIRGYSGKITEFLLGYDAKVVLIACNSASASAYEALLAEYGNRVIILDVINP
                     VVEYCASRNFQRLGVIGTKVTVNSGTYTRKLKEASPTTSVVSLATPLLVPMIEEGFIF
                     DDISNAIIRTYLSDQKLNDIDGLILGCTHYPIIRNQISKFFNFSVEVVDSARIVAVRL
                     REILEEHNLVNRGKPGEDLFFVSDFTDYFEKIARMFFESSINLRRADIWS"
     gene            complement(5758..6270)
                     /locus_tag="PLA17_03190"
     CDS             complement(5758..6270)
                     /locus_tag="PLA17_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPK84447.1"
                     /translation="MKKVFGILLFAAMFFMAQDGFAQSFKTGHINRDEIITSMPEYDT
                     AMNKYNEFGQELTNALELMQVELNNKYDQFTKEEKNLSELVKANKVQEITDMQTRMNN
                     FQQQAQLQLQEKQAELLNPIIEKANNAINAVAKEGGYIYIYDIRTLVYVDTVKSTDIG
                     PLVRARLGIK"
     gene            complement(6310..6825)
                     /locus_tag="PLA17_03195"
     CDS             complement(6310..6825)
                     /locus_tag="PLA17_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPK84448.1"
                     /translation="MRKIIIMAVIVSAATLSGMAQKFAFVDSEYIRNNIPAFTAAQQQ
                     LEKLSRGWEKEVADGYAAVEQMYKDYQAEVVLLSQDQKKKREEAIIAREKEVKELQES
                     YFGMKGELYKKREELVKPIQDEILKAIKEISVEGSYAVIFDTSAGSNILFANPRYDLS
                     DQVLQKMGYKN"
     gene            complement(6852..9374)
                     /gene="bamA"
                     /locus_tag="PLA17_03200"
     CDS             complement(6852..9374)
                     /gene="bamA"
                     /locus_tag="PLA17_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPK84449.1"
                     /translation="MKKMKPMRILSLFALLLTGLIATAQTEEKYVSYTYPEEYTLAGI
                     TISGVKYLDPNALISLSGLRIGQKLDIPGELISAAAKKLMDQGLFSDVRITVTGKEEE
                     NIWLDIHLQERPRLSSLRFEGIRKSEETDLTEKLSLPPGSQVTAHLLNRAERTIRNHY
                     IEKGFLNAAVDIIQKDDPDLPNNVILNFVVAKNAKVKIEQIIFEGNDYFMDKRLRRVM
                     KNTKQKDLNIFKGSKFIEDKYEEDRAKLVEFYNENGFRDFRILGDSTYALEDGRIALV
                     IKVNEGNQYYLRNVDWVGNSIYTKDQLSRVFNFPSGEIYNQTMIDKRLNVDDDAVSSL
                     YLNYGYLFSRLTPVEKKVEGDSIDLEVRIYEGEPATLNNIIITGNTRTNEHVARRELF
                     TYPGDLFSKDKLIRSARQVAVLGHFDPEKINPQPLLNQVEGTVDLLYALEEKANDQFE
                     ISGGWGQGMLVGTVGVRFNNFAMRNFFKWNEWRPYPSGDGQSLQIRAQSNGSYYQSYN
                     ISFIEPWLGGKKPNTLSISAFRNKMSESKKSLEEDRQSMIIDGGSIGFGKRLEWPDDY
                     FSVFYEASFQRYNLNKFDRFQFLFKTGTANLATLSAKITRFSAGPNPIYPTGGSTFTL
                     SLQATPPFSAISGKSFAGLTDQEKYKWIEFYKAVFKADYYLPLDGAGKLILNTKAAFG
                     YLGFYNKDIGPSPFENFYLGGDGMTGYSFYGREVIALRGYTNGSLTPWIDKLEADGKI
                     RRVESGNVYSKLTVELRYPISLNPQATIFALAFLEAGRAWHNIRDYNPFRMNRSAGVG
                     LRANLPMFGLLGIDWGYGFDLPVTGRSEDAGSQFHFVIGQQF"
     gene            complement(9376..10125)
                     /locus_tag="PLA17_03205"
     CDS             complement(9376..10125)
                     /locus_tag="PLA17_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPK84450.1"
                     /translation="MTFKDQIDLNRLPVHVAVIMDGNGRWAHMRGRERVFGHHEGVVA
                     VRKTVEASAELGVRYLTLYAFSTENWNRPDDEVSALMDLMIESINNETRTLIEKSISL
                     RVIGDLSRLSEKVRSRLFETMQITSGGTRMMLVVALSYSSRWEISEACRRIAVEAREG
                     RLNPDQIDEHTIGTYLTTSGIPDPELMIRTSGEQRISNFLLWQLAYTELYFTEKLWPD
                     FGKEDFYEAVADFQKRERRYGKTSEQIAENT"
     gene            complement(10128..10775)
                     /locus_tag="PLA17_03210"
     CDS             complement(10128..10775)
                     /locus_tag="PLA17_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPK84451.1"
                     /translation="MKRSIIITLLIILALPALPQRNADYGVSAGVVNYLGEQNPLNAF
                     FKPGPAVQLFYRYNFNPRQAVRANLLSGYMTPLGSLLGELGATYEFNFYPYSTFSSRR
                     IDYSPYIAGGAAFSMMSKAGPFLSIPFSAGFKVNVANQVGVEIEYGFRKTFTDKLDGQ
                     SYITDPGGEPVDGTWTHNNDWYSFLGVSVTWKMYNRLAGCPAFAEMEKDKKRKRR"
     gene            complement(10772..11500)
                     /locus_tag="PLA17_03215"
     CDS             complement(10772..11500)
                     /locus_tag="PLA17_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPK84452.1"
                     /translation="MKRYEATAAIGTSQFFGDIGGYSVGENFLGLKDITFRQTRFSVN
                     GSFRYFITDNVAARLSFTYCMFHADDARGANEPRGYKTSSFLYEPALFGEYYLVRNRE
                     RNSFLFQTYRYRRASRLRDFFRSVDVYALTGIGGAGYNVFYGNEKMQDRWDKEPSLKR
                     SGFTAVIPLGVGAKLAIDPNLLIGVELAGRYALSDYLDGYTTDDFSRRNDAYHTFSVT
                     VNYRIKTARNGLPDLRSLRQSPRL"
     gene            complement(11650..12528)
                     /locus_tag="PLA17_03220"
     CDS             complement(11650..12528)
                     /locus_tag="PLA17_03220"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPK84453.1"
                     /translation="MDKTIALYSKSLTEKTTAGLMRVIDALYDRGVTIYLNIMDPAGN
                     HFKHDHRVILFSELSDLPANVFMILSIGGDGTFLETARRVRTSGIPVAGINTGRLGFL
                     ANIKDNEIGKSVDMIISGNYELISRSMLEITFPPELAAGGNAVALNEITVQKGDQSMI
                     TITVEVDGTFLNTYRADGLIVSTATGSTAYNLSVGGPILYPTDDSIIIVPMSPHNLTV
                     RPIIVSGDSVITLVTGGRSSQCMVTIDSKAIRVPSGTVLEVRRSASMLSTVTIAGNDF
                     FSTLRNKLMWGADPRN"
     gene            complement(12541..13206)
                     /locus_tag="PLA17_03225"
     CDS             complement(12541..13206)
                     /locus_tag="PLA17_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPK84454.1"
                     /translation="MLAKDMISDVVPALKTSETAQTALNWMETFRVSHLPIVNESDYL
                     GLISDSDIYDLNQPEEPIGNHPLSLPRPSVKMHQHIYEVIALAARHKLTVVPVIDEDN
                     HYRGSITGNDIIRGIASISAIDQPGGILILKMLQRDYSMSRISQIVESNDARILSMYI
                     TSDPDSTSLEITLKVNTTDLVSIIKTFERYSCEVTSWVTDNDELDRFYRDRYNLLMKY
                     LNM"
     gene            13292..14011
                     /locus_tag="PLA17_03230"
     CDS             13292..14011
                     /locus_tag="PLA17_03230"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPK84455.1"
                     /translation="MTRLSVNINKVATLRNARGGNVPDVKLAAIKCQSFGAGGITVHP
                     RPDERHIRYSDVYDIRDVVTTEFNIEGNPEEQFIRLVLDVVPDQVTLVPDAHDAITSN
                     AGWDTRKNLSFLTDVIARFHGKGIRTSIFTGTDPEIIEYAARTGTDRIELYTEPYASG
                     YHADRKAAVAPFVTAAIVARNAGLGVNAGHDLDLHNLAWLHSNIPWLMEVSIGHALIS
                     DALYYGLENTIQMYLRELRRS"
     gene            complement(14025..14633)
                     /gene="udk"
                     /locus_tag="PLA17_03235"
     CDS             complement(14025..14633)
                     /gene="udk"
                     /locus_tag="PLA17_03235"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPK84456.1"
                     /translation="MLIVGIAGGTGSGKTTVVRKLIDMLPQGEVVVLPQDSYYRDNSH
                     IPVEQRQSINFDHPDSVEWPLLISHLAELKAGRPIMMPIYSYLTCVRSEETVPVRPAE
                     VVVVEGILVLADPGLRQMLDIKVFVDADADDRLGRVIQRDIIERGRSVLMVLERYNKT
                     VKPSHLQFIEPSKRYADIIIPGGGENLVGIEVLKTIVEKHLR"
     gene            14675..14977
                     /locus_tag="PLA17_03240"
     CDS             14675..14977
                     /locus_tag="PLA17_03240"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HPK84457.1"
                     /translation="MVKHIVIFKLAQPVTPEKRNQSVMKLKEIFGPLGNRLKYIVEYR
                     TGVNISDVDHAGDFVIDATFASLDDLRRYQSSELHREAVAAASIIKKAKLVVDYTF"
     gene            14983..15981
                     /locus_tag="PLA17_03245"
     CDS             14983..15981
                     /locus_tag="PLA17_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPK84458.1"
                     /translation="MSSLYPIRFKPMLRELLWGGDALRERFGKKAKPGTKIGESWEIC
                     GMPGNSSVVANGFLKGNSLEEIAEIYMDELLGEAVFAKFGTEFPLLVKLIDAADKLSI
                     QVHPDDRLASERHHAWGKTEMWYVIDAKPGSVIYTGFRKKTTREEYLEHLEAKKLAEL
                     INVTPVRSGDVFFIPAGLVHAIGGGVLLAEIQQTSDVTYRIYDWDRVDTSGRPREMHT
                     SLALDAINFSFDSTNLIRTSPELNRTVLLAESQYFHTSLITFDRPIIKDYSLTDSFII
                     YICTQSLAVVECNGHSEEIKAGETILIPASADSVAIIPRGTATLLEVFIPQKNRSK"
     gene            15978..16739
                     /locus_tag="PLA17_03250"
     CDS             15978..16739
                     /locus_tag="PLA17_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84459.1"
                     /translation="MKQLTLLTAAIIMILASACGGGAGQEGSGRKKDAHEADTGYTGI
                     KNYIKDNIKLKEVTYKNGIREGMTRTFYKGGQLEQEIPYSGDSKNGEAKWFYTDGKLF
                     RTTPFVNDTIHGDQVQYYKNGGVKARLSYTDGKRHPSLEEFLMSGEKVTDYPALRYRV
                     NDRYAERGLYKIFVEFDNMAENGKFYRGDYINGLVDLDSLNPMLQTVTTGYLDLQKRP
                     GHKADSVVVIASYLTQFGNRLYYRLAIPLPYKDLN"
     gene            16745..17131
                     /locus_tag="PLA17_03255"
     CDS             16745..17131
                     /locus_tag="PLA17_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPK84460.1"
                     /translation="MSQILVPGIRLTTTRTVSKNDTAAVYGSGLHDVLSTPAMVAFME
                     QTAMQAVEACLNEGEGTVGTEVNIRHLKATPVGMTITCTATLQEVNGNRLLFHVEADD
                     EIGKIGEGQHERFIINNQRFKEKLNG"
     gene            17131..17739
                     /gene="yihA"
                     /locus_tag="PLA17_03260"
     CDS             17131..17739
                     /gene="yihA"
                     /locus_tag="PLA17_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPK84461.1"
                     /translation="MIIRSASFVTSSARISQLPPPDLPEYAFIGRSNVGKSSLINMLV
                     SRKGLAKTSGSPGKTQTINHFIINESWYLVDLPGYGYARVSQKMRDQWEKLITSYITM
                     RENLILLFVLIDGRHEPMASDLAFMERLAMNGIPFARVFTKTDKLSAAEAAKIREVHD
                     RVMLRSWESLPPAFVTSASRGTGREAVLDYIESNMIFFTGMK"
BASE COUNT         4341 a         4490 c         4435 g         4600 t
ORIGIN      
        1 gaggacagag gagggagcca cgctacagtg cgtgaggaca gaggagggag ccacgctaca
       61 ttgcgtgagg atggaggagg gagccacgct gtattgcgtg aggatggaag agacagaaaa
      121 caattatgtt gaatactacg gagaactatg gaaaaactta atgacggcag cggaaagctg
      181 tctgacggct acaacaagat agagaaatgg aactctgagg tcatagagga gatcgcagga
      241 tattactaca agatactaag gcttctggga gaaaaccctg acagggaggg gttacttaag
      301 acccccgagc gcgttgccaa ggcattcagc ttcctgactc gtggttacta ctctgaaccc
      361 gaggagattc tgcgcagcgc catcttcaag gaggagtacc ggcagatggt gattgtgaaa
      421 gacatagaca tttactcact gtgcgagcat cacatgctgc cgttccacgg caaggcgcat
      481 gtggcgtaca ttcccgatgg ttatatcacc gggctgagca aaatagcaag ggtggttgaa
      541 acctatgctg cccggctcca ggtgcaggag aggctgacaa cacagatacg aaactgtatc
      601 caggaaaacc tgaagcctct tggtgtggcc gtagtcattg aggcacagca tatgtgcatg
      661 cagatacgag gtgtgcagaa gcagaactcg gtcactacaa cctcggcttt caccggatgt
      721 ttcctctcta acatgaatac acgcgaggaa ttcatgcacc tgatagccaa taagttacac
      781 tgaatttatt tattcaccca acaactgaat ccaaatatga aggcttatgt attccccggt
      841 cagggagcgc agtactccgg catgggaaag gatttgtatg agaaatttcc cgtggcgcat
      901 gatatgtttg aaaaagccaa tgctttgctt ggcttccgca ttacggacat catgttttca
      961 ggcaccgacg aggagctgag gcagacgcgt gtgacgcagc ccgcgatttt tcttcactcg
     1021 gtgatcctga cggctgtggc cggaagtagc tttaaaccgg cgatggttgc cggtcactcg
     1081 ctgggggagt tttcggcgct ggtagctgcc ggagctctct cctttgagga gggactggcg
     1141 cttgttgcca gaagggcctc cgcgatgcag aaggcctgca acatcaaccc gtctaccatg
     1201 gccgcagtgc tgggcatgga tgatgcagtg gtggaagagg tatgtgccgc gctgaacggc
     1261 attgtggtgc cggccaacta caacagtccc ggccagatag tgatctccgg caccatggaa
     1321 ggggtgtcag cagccactga ggagctgaag aagaggggag caaaacggat cgtgccgctg
     1381 tcagtgagcg gcggatttca ttcgccaagc atggagcctg cccgccagga tctggaagag
     1441 gccatacgga aggcacgctt cagccgcccg ctgtgccccg tctaccagaa tgtgacaggc
     1501 atgccggtga cggaacctga tatgattatg gtgaacctga ttgcacagct gacctcgccg
     1561 gtgaggtgga cacagaccat acagacgatg attgccgacg gtgtgacgga gttcattgag
     1621 gcggggccgg gcaacgtgct gcaggggttg atcaggaaga taaatccgaa cgttgccgca
     1681 tcatcgattg acccaatgtg atgtgtggac tgttgctgac ggtcatgtac gttgcgcata
     1741 cagccgatcc cctgctttca aggaaccggc tgtttttcat cacaccgggc agagcccggc
     1801 aacggcgttg ttttagtggt gattacttca cctcccaggt tgctccgctt ttgagcaatt
     1861 catctactgt tctgatcttt gatctggcct gtacccgctc atgctgctcc atcatggctg
     1921 cgtcataggt ttttgtactg atggccctta taacgccaag cgccacaggg tattcaggaa
     1981 gcgacatgcc tgcgagcctg tagtgaatgc ccgggttggg ctcatgtgca tcatgggtaa
     2041 ggatatcctt ctctgtaata ccgtcctcgc caatctttac cactttcagc ttgaggtcct
     2101 tcaggatgat gcccttatcc cggttcttgc cgaagatcat cttttcacca tgacggagaa
     2161 tgatggtatt ctcatcgcgg ttctccttgc tggtgaaggc gtcatgagtt ccgtcattga
     2221 agatgacaca gttctggaca acctccacaa cggccgtgcc gtggaacttt gctgcctcct
     2281 tgtaaacctc acttgagagc tgcaggttat agtcaaccgt acgggcgaag aagcgtccgt
     2341 tggcacctat tgtcagttcc cctaccttga agggttcttc tattgtgcca tacggcgaag
     2401 ttttggttat gagtcccttt ttcgaagtag gagaatactg ccctttggta aggccataaa
     2461 tctcgttgtt gaagaggacg atcttgatgt caatattacg ccttatagcg tgtatatagt
     2521 ggttccctcc gattgcgagt gcgtcgccgt caccggtcat ctcccatacc atcaggtcag
     2581 ggtttgcaat ctttataccg gtggctattg ctgccgccct gccgtgaata ccgtgaaacc
     2641 cgtaggtatt catgtaatac gggagccttg aagagcaacc tatacctgac acgaagacat
     2701 atttttcctt cggtatatca agttcagcaa gagtcttctg aagtgcggcc agaatggcat
     2761 ggtcaccgca tcccgggcac catcttactt cctgatcgct tttgaagtct ttcgctgcat
     2821 atttattatt atttgtttca gccatttttc ttacccttaa gcagtttaac acaatgatct
     2881 ttaatctccg tgattgtgaa cggaagcccc tgaaccttgt tgtactggtt gtacgtgtac
     2941 ttctggtgct taattctcag gtagttggca aactgacctg tattaagttc acagaccagc
     3001 agtttgtcaa atcctgcaaa cacctttgca gtgtttttcg gcaggggtct gatgtagttg
     3061 aagatggctg ccgatactgc gtatccctcc ttctgcaatt cagtcacggc ggaagtaata
     3121 tgcccatagg tgctgcccca tccgataacg agcagatcac cggttttctt tccgaggacc
     3181 tccaggttgg gcaattcatt tacaatccgc tccacctttt cttcacgggt tttgaccatg
     3241 tactcatggt tctccgggat gtatgatacg ctccccttga ttgtcttctc gagtccgcca
     3301 acacgatgct ccagtcccgg tgtgccgggg atggcccatt tgcgtaccag tgtcttgggg
     3361 tctctctcgt atgcaaggaa ctccttgtca ctcctgactg cgtatggtac ggtgattgat
     3421 ggaaggtcct tggttgatgg tatcctccat ggctccgatc cgttggccag atagccgtca
     3481 gtaagcagga tcaccggggt catgtgttcg atggcaattt ttgctgcctc gaatccgaac
     3541 ctgaagcagt ctgatggtgt gctggctgca atgaccggta tcgggctttc gccgttacgg
     3601 ccatagagtg cctgccacag gtcggcctgc tcggttttgg ttggcagccc cgtggagggt
     3661 ccgccacgct gaacgtcaat caccagcagt ggcaattcag tcatcacggc caggttaagg
     3721 gcttcagact tcagtgcaag gccggggccg gatgttgaag tgacagccat gcgccctgcg
     3781 aaactggctc cgatggaggt gcaaataccg gctatctcgt cctcagcctg gaggctcttg
     3841 acacccatat ccttccttag cgccagttcc tcaaggatac cggttgccgg ggtgatggga
     3901 tatgaaccaa tgaagagctt cagaccggct ttctcagcag cggcaagtac gccccatgcg
     3961 gttgcctggt tgccgttgat gttgcgatac gtgcctttct tgattggcgc acgattaacc
     4021 ctgtatgaag gtgtcagtgc ctcaatggtt tctccgtagt agtaacctgc cctgagcacc
     4081 ttgttgttgg cctcagccac gagcggcttc ttgccgaatt tctcctcaat gaactcctca
     4141 gtgtacttaa gcctgctgtc aaagagccag tagatcagcc ccagtgcaaa catgttcttc
     4201 gtcctgagta cggtcttggg gtcgaggtcc atatccttga gggcttcctt caccagggtg
     4261 gtgataggcg ccgcaatgag gttcatagag tcaagcttgt cttctgcgaa cgggtcagcc
     4321 ttgtagcctg ccctctgtat cgacttttca gtgaagttat ctgagtcata aatgattgtg
     4381 gaccccttct tcagccacct tacattggca cggatggcgg cggggttcat cgcaacaaga
     4441 atgtctgtga aatctcccgg agtgttaatc agtttgtgcc cgatgtgtac ctggaatccg
     4501 gagacgcctc cgacggtacc ctgcggagcc cttatctctg caggatagtc cggaaaggtg
     4561 gagatgtcat gaccaaacag ggccgacgtg ttggagaaca gggtgcctgt aagctgcatc
     4621 ccgtctcccg agtcgcctga gaacctgatc accaccgcat ccttctcaat gacctttgat
     4681 ctttttccca tgatgattat tatctatttg attttgagta agtacccgta taaaatatca
     4741 acctgaaaag atgaagctaa gttcaccatt aacctgatac aactgtatga ttttggtcat
     4801 gttataccat gaccttccta atacctgatc aggatagaag atccctgcga aaaaaacatg
     4861 ccgatgtctc tgtggaaagc tatttgccag gtatcatgac cagatatctg ccctgcggag
     4921 gttgatggag ctctcaaaga acatcctggc aatcttctcg aagtagtctg taaaatctga
     4981 cacaaagaac aggtcttcac ccggcttgcc cctgttgacc aggttatgct cctccaggat
     5041 ttcccggagc ctgactgcaa caatacgtgc tgaatcaacc acctccacgc taaagttgaa
     5101 gaacttgctt atctggttac ggatgatggg gtagtgtgta catccgagta tcaggccgtc
     5161 aatgtcattc agcttctggt cagatagata ggtgcggatg atggcattgc tgatgtcatc
     5221 aaagataaag ccctcctcaa tcatgggtac gagcagcggc gttgcaagtg agaccacgct
     5281 ggtagtgggt gatgcctcct tcagcttcct ggtgtatgtg cctgaattaa cagtcacttt
     5341 ggtgcctata acccccagcc tctgaaagtt gcgtgaagcg caatattcaa ccaccgggtt
     5401 tatcacatcg agtataatga ccctgttgcc gtattctgca agcaatgcct cgtatgctga
     5461 ggctgatgcc gagttgcagg caatgagtac cactttggca tcataaccga ggagaaactc
     5521 ggttatcttc cccgaataac ccctgatggc ttcttctgac ttgtcaccat aaggcaggtg
     5581 cgcagtatca ccaaaatata caatctgttc cccgggcagt atctggcgga tggcctgtgc
     5641 caccgtcaac cctcccacac ctgagtcaaa caccccgatc ggccggttgc cagtttccat
     5701 gactttataa tgtaagactg cccgccaggg gcgggcagca ctttgatttc attttaatta
     5761 tttgattccc agcctggccc ttaccagcgg gccgatgtca gtgctcttta ccgtatcaac
     5821 gtaaacgagg gtgcggatat catatatata tatgtaaccg ccctcctttg caacggcatt
     5881 gatagcgtta ttggccttct caatgatagg attcagaagc tcagcctgct tctcctgcag
     5941 ctgtaactga gcctgctgct ggaaattgtt catgcgtgtc tgcatgtcag ttatctcctg
     6001 caccttgttt gcctttacaa gttcagagag attcttctcc tccttcgtaa actgatcata
     6061 cttgttgttc agttcaacct gcatcagctc aagggcgttt gtcagctcct gaccgaattc
     6121 attgtattta ttcatggcag tatcatactc aggcatcgac gtaatgattt catcgcgatt
     6181 gatgtgacct gtcttgaacg actgcgcaaa tccatcctgt gccatgaaaa acattgcagc
     6241 aaacaggagt atcccaaata cttttttcat tgtcttaatt ttcaaattat aacgtgcaaa
     6301 tgtaattatt taattcttat atcccatttt ctgcagaacc tgatctgaga ggtcatatct
     6361 cgggttggcg aagaggatat tcgaacctgc ggatgtgtca aaaatcactg catagctccc
     6421 ctcgacagat atctccttta ttgctttcag tatctcatcc tggataggtt ttaccagttc
     6481 ctcgcgtttt ttgtagagct cgcccttcat tccgaaatag ctctcctgaa gctcttttac
     6541 ctccttctcc ctggcaatga tggcctcttc cctcttcttt ttctgatcct gtgagagcaa
     6601 caccacctca gcctggtaat ccttgtacat ctgctccact gccgcataac cgtcagccac
     6661 ctccttctcc catcccctgg aaagtttttc aagctgctgc tgtgctgccg taaaagcagg
     6721 tatgttattg cgtatgtatt cgctgtcaac gaacgcaaat ttctgtgcca taccgcttaa
     6781 cgtggcggca gacacaatta ctgccatgat aatgatcttt ctcattgctg ttgtatttga
     6841 tcttgtttta atcagaattg ctgacctatt acgaagtgaa actggcttcc ggcatcttcg
     6901 gatcttccgg tgacaggtaa atcgaaaccg taaccccagt caatgccaag gagaccaaac
     6961 atgggcaggt ttgccctcag gcctaccccc gccgagcggt tcatgcggaa aggattgtag
     7021 tccctgatat tatgccaggc cctgcctgcc tcgagaaagg ccagtgcaaa aatggttgcc
     7081 tgcgggttca gcgagatagg gtaacgcagc tccacggtga gcttcgagta aacgttaccg
     7141 gattccaccc gtcttatttt tccatcggcc tcaagcttat caatccatgg tgtaagtgat
     7201 ccgttggtgt aacccctgag ggctataacc tcgcggccgt aaaagctgta tccggtcatg
     7261 ccgtcacctc cgagataaaa gttttcaaag ggcgaaggcc cgatgtcctt attgtaaaat
     7321 cccaggtacc cgaaagcagc tttggtatta agaatgagct tgcctgcgcc atcaaggggc
     7381 aggtaataat ctgccttgaa gacagccttg tagaactcta tccacttgta cttttcctgg
     7441 tcagtgaggc ctgcaaagct ttttccgctg attgctgaaa agggcggtgt ggcctgcagg
     7501 gagagtgtga aggttgaacc tccggtcggg taaatggggt tgggccctgc cgagaacctg
     7561 gtgattttgg cgctcagcgt tgccagattg gccgtaccgg tcttgaaaag aaactggaac
     7621 ctgtcgaact tgttcagatt gtatctctga aagcttgctt catagaatac cgagaagtag
     7681 tcatcaggcc attccagcct cttgccgaag cctatcgaac caccgtcaat gatcattgat
     7741 tgcctgtctt cctcaagtga tttcttgctt tcactcatct tgttccggaa tgcagaaata
     7801 gagagggtgt tgggcttctt gccaccgagc catggttcga tgaatgagat attatagctc
     7861 tgataataac tgccgttcga ttgtgctctg atctggagac tctgtccgtc acctgacggg
     7921 tacggtctcc actcgttcca cttgaagaag ttgcgcattg cgaagttatt gaatctcacc
     7981 cctaccgttc ccacgagcat tccttgtccc catccgcctg aaatctcaaa ctggtcattg
     8041 gctttttcct cgagcgcata tagcaggtca acggttccct ccacctggtt gaggagaggc
     8101 tgggggttga tcttttcggg gtcgaaatgc cccagtacgg ctacctgcct tgctgaacga
     8161 atgagtttat ccttgctgaa gaggtcaccc gggtatgtga acagctcacg cctggccaca
     8221 tgttcatttg tgcgggtatt accagtaata atgatgttat tgagagttgc cggctcacct
     8281 tcgtagattc gcacttcaag gtcaatggag tcaccctcca ccttcttctc aacgggagtg
     8341 agccttgaga agagatagcc gtaattgagg tagagcgaac tcacggcatc atcatccacg
     8401 ttaagacgct tgtctatcat ggtctggttg tatatctcgc cggaggggaa gttgaagaca
     8461 cgcgacaact gatcttttgt atagatgctg ttgccaaccc agtcaacatt ccgaaggtaa
     8521 tactgattac cctcattgac cttgatcacc agtgctatcc tgccatcttc cagtgcatag
     8581 gtggagtcac ccagtatgcg gaagtctcga aatccgtttt cattgtagaa ttcaacaagc
     8641 ttggcacggt cctcctcata cttgtcttcg atgaacttgg agcctttgaa gatattgagg
     8701 tccttctgct tggtgttctt catcacacgg cgaaggcgct tgtccatgaa gtagtcattg
     8761 ccttcaaaaa tgatctgctc aatcttaacc ttggcattct tggcaacgac gaaattgagg
     8821 atgacattat tgggcagatc gggatcatcc ttctggatga tgtcaactgc ggcgttaagg
     8881 aatccctttt caatataatg attcctgatt gtacgttcgg ccctgttcag caggtgtgcc
     8941 gttacctgtg aacccggagg gaggcttaat ttttcagtca ggtctgtctc ttccgatttc
     9001 ctgatccctt cgaacctgag cgaggagagc ctcgggcgct cctggagatg tatatcaagc
     9061 cagatattct cttcctcttt cccggtaacg gttatcctta catcagagaa gagcccctga
     9121 tccatcagtt tctttgctgc tgccgaaatc aactcaccgg gtatatccag tttctggcct
     9181 atcctcagtc cggagaggct tatcagggca ttgggatcaa ggtatttcac ccctgagatg
     9241 gttatgccgg caagagtgta ctcttcgggg taggtatagc tgacgtattt ttcctccgtc
     9301 tgggctgtgg caatcagtcc tgtcagaagc aaggcaaaaa gagatagtat ccgcataggt
     9361 ttcatttttt tcatctcagg tgttttcggc aatctgctca ctggtcttcc catatctcct
     9421 ttcccttttc tgaaagtctg ccactgcttc gtaaaaatcc tccttaccaa aatcgggcca
     9481 aagtttttca gtgaaataaa gttcagtata tgcaagctgc cagagaagga agttgctgat
     9541 acgctgttct ccgctggtac gtatcatcag ttcgggatca ggaatacctg atgttgtcag
     9601 atatgtgcct atggtatgct catctatctg atcaggattc aatctaccct cccttgcttc
     9661 aactgctatc cggcggcatg cctccgaaat ctcccagcgc gatgagtaac tgagagcgac
     9721 aacgagcatc atccgtgtac caccggatgt gatctgcatt gtctcgaaca gcctgctcct
     9781 tactttttcc gaaaggcgtg agaggtcacc tatgaccctg agagagatac tcttctctat
     9841 cagggttctg gtctcattgt ttattgactc aatcatcagg tccatcaggg ctgagacctc
     9901 atcatcaggc ctgttccagt tctctgtgga gaaagcatac agagtaagat acctgactcc
     9961 cagttcggca gaagcctcca ctgtcttgcg cactgccacg accccctcat gatggccgaa
    10021 gaccctttca cggcctctca tgtgtgccca gcggccgttg ccgtccataa tgacagccac
    10081 atgcaccggc aacctgttca aatctatctg atccttaaaa gtcaacgcta tctcctcttt
    10141 ctttttttgt ctttctccat ctctgcaaag gcaggacaac ctgcaagcct gttgtacatc
    10201 ttccaggtga ctgaaacccc caggaaacta taccagtcat tattgtgggt ccatgttccg
    10261 tcaaccggtt cgccacccgg atctgttata taggactgac catccagttt atcggtaaaa
    10321 gtcttgcgga atccatattc gatctcaacg ccaacctggt tagccacgtt gaccttaaaa
    10381 ccggcagaga atggaatgga gaggaatggg ccggccttgc tcatcattga aaaggccgcg
    10441 cctccggcga tatatggtga atagtctata cgcctgctgc tgaatgtgga gtatgggtaa
    10501 aagttgaatt cataggttgc gcccagctct cccagcaatg acccgagcgg tgtcatgtaa
    10561 ccgctcagga ggtttgccct tacggcctgt ctgggattga agttgtaacg gtaaaacagc
    10621 tgtacggcag gaccgggttt gaagaaagcg ttgagagggt tctgttcccc caggtagttc
    10681 accaccccgg ctgagacacc gtaatcagcg ttgcgctgag gcaatgccgg aagagccaga
    10741 attatcagta gggttattat tattgatctt ttcataaccg tggtgattgg cgcaatgatc
    10801 tgagatcagg caaaccgttg cgcgcggttt taatcctgta gtttactgtc acgctgaagg
    10861 tatggtaagc atcatttctc ctcgaaaaat catccgtggt ataaccgtcc agataatcag
    10921 agagagcata cctgcctgcc agctccaccc ctatcaggag gttgggatca atggccagct
    10981 tggcgcccac acccagcgga atcacagcgg tgaatccgct cctctttagt gacggttcct
    11041 tatcccacct atcctgcatc ttctcattac cgtaaaagac gttatatccg gctccgccga
    11101 taccggtcag tgcatagaca tcaacactcc tgaaaaagtc gcgcagcctg cttgccctcc
    11161 tgtaccggta ggtctgaaag aggaagctgt ttcgctcacg gttcctgacc agataatact
    11221 ctccaaacaa ggccggttcg tacaggaagc tgctggtttt gtagcccctg ggttcatttg
    11281 ctcccctggc gtcatcagcg tgaaacatgc agtaggtgaa tgagagcctt gcagcaacgt
    11341 tgtcagtgat aaaatacctg aaagacccgt ttacgctgaa gcgggtctgc ctgaatgtta
    11401 tatccttgag cccgagaaag ttttcgccaa cactgtaacc tccgatgtca ccaaagaatt
    11461 gtgatgtgcc gatagccgcc gtggcttcat atcgtttcat cttccacagc tgacctgata
    11521 cgaatggcgc cgtgaaagac accaccatac acatcactat cagacgctta accatcagtc
    11581 acattgcaat tgcggctaca aatataagga atgtccaata tttaaaactt aaatattcct
    11641 taattattgt cagttacggg gatcagcacc ccacatgagt ttatttctga gggtgctaaa
    11701 aaaatcattt ccggcgatgg ttaccgtgct gagcatggaa gccgaccgcc gcacctcaag
    11761 aacggtgcct gacggtaccc ttatggcctt tgaatcaatg gtaaccatgc attggctgct
    11821 ccggcctccc gtgaccaggg tgataacact gtcacctgag acaattattg gcctcacagt
    11881 caggttatgc ggtgacatgg gaacgattat tatactgtca tcagtaggat aaagaatggg
    11941 gccacccaca ctcagattgt aggccgttga tcctgtagcc gttgagacga tcagtccgtc
    12001 ggccctgtaa gtattcagga aggtaccatc aacctcaaca gtaatagtga tcatgctctg
    12061 gtcacctttc tgtacggtta tctcattcag tgccacggcg ttaccacctg ctgccaattc
    12121 cggcgggaaa gttatctcga gcatcgagcg gctgataagc tcataattgc ccgaaataat
    12181 catatcaact gacttcccta tttcattatc cttaatattg gccaggaagc caagacgccc
    12241 ggtgttgata cccgctaccg gaatgccgga agtacgtacc ctcctggctg tctcaaggaa
    12301 agttccgtca ccgcctatgc tcagtatcat gaacacattg gccgggagat cagacaactc
    12361 gctgaatagt atcaccctgt ggtcatgctt gaaatggttc cctgcagggt ccatgatgtt
    12421 caggtaaata gtcacacccc tgtcatagag ggcatcaatg accctcatca ggcctgcagt
    12481 ggtcttttcg gtaaggctct tgctgtacag tgcaatggtt ttgtccatct tgatagcggg
    12541 ttacatgttc aggtacttca tcagcaggtt gtacctgtct ctatagaaac ggtcaagttc
    12601 atcattgtca gtcacccagg atgtgacctc acatgagtat ctctcaaatg tctttatgat
    12661 ggaaacaagg tcggtagtat ttaccttcag tgttatctca aggctggtgg agtcagggtc
    12721 agatgtgatg tacatgctga gtatccttgc atcattgctc tcaactatct gtgatatcct
    12781 gctcatggag tagtcgcgct gaagcatctt cagtatcagt atcccgcccg gctggtcaat
    12841 ggctgagatt gatgcgatac ctcttattat atcattgccg gtgatggagc cacggtaatg
    12901 gttatcctca tcaatgaccg gcacaacggt aagcttgtgc cgtgctgcaa gcgcaatcac
    12961 ctcatatata tgctgatgca tctttaccga gggtctgggc aatgaaagcg ggtggtttcc
    13021 tataggctct tccggctggt tgaggtcata gatgtcactg tcagagatga ggccgagata
    13081 gtcactctca ttcacgatgg gaaggtgtga caccctgaaa gtctccatcc agttaagtgc
    13141 agtctgcgct gtttcagatg ttttcagtgc aggaaccacg tccgatatca tatcttttgc
    13201 aagcattcag cagctctttg gcgaataaaa gtacaaaaaa ctgcggatct ttataacttt
    13261 gcccgcggaa tcagctgaag agaagagagg catgacaaga ctgagtgtaa atataaacaa
    13321 ggttgccacc ctgcgcaatg cccgtggcgg taacgtacct gatgttaagc tggctgccat
    13381 caaatgtcag tcattcggcg ccgggggaat caccgttcac ccgaggcctg acgagaggca
    13441 tatccgttac agtgatgttt atgatatcag ggatgtagtc acaaccgagt tcaacatcga
    13501 gggcaatcct gaggaacaat tcatcaggct ggtgcttgac gtggtgcctg accaggtgac
    13561 tctggtaccc gatgcacacg atgcaataac ctcgaatgca ggctgggata ccaggaagaa
    13621 cctctccttt cttaccgacg tgattgcccg gtttcacggg aaggggatac gtacctccat
    13681 cttcacaggc actgatccgg agataataga atatgcagca cgcaccggca ctgaccggat
    13741 tgagctctat accgaaccct acgcatcagg gtatcatgct gacaggaaag cagcggtggc
    13801 ccctttcgtg acggcagcca tcgtggcacg caatgccgga ctgggcgtaa acgcaggtca
    13861 tgaccttgac cttcacaatc ttgcatggct gcacagcaat attccgtggc ttatggaggt
    13921 ctccataggg catgcactta tttctgacgc cctctactac gggctcgaaa ataccattca
    13981 gatgtacctg cgcgaattgc gcagaagctg acagaacgcc accattatct caggtgtttc
    14041 tcaactatgg tcttcagcac ttctatccca acaaggttct cgcctccacc cgggataatt
    14101 atgtctgcat atctctttga cggctcaata aactgaaggt gagagggttt caccgttttg
    14161 ttgtatcgtt cgagtaccat cagcaccgag cggccgcgtt caattatgtc acgttgtatc
    14221 accctgccca gccggtcatc cgcatctgca tccacaaata cctttatatc aagcatctgg
    14281 cggaggccgg gatcggcaag aacaagtatc ccctccacca caaccacctc ggccggcctg
    14341 acgggaacgg tctcctctga cctgacacag gtaagatatg agtagatagg catcatgatc
    14401 ggcctgccgg ctttcagctc agccagatgg gatatcagca gcggccactc aaccgagtca
    14461 ggatggtcaa aattgatgct ctgcctctgt tcaacaggta tgtggctgtt atcacggtaa
    14521 tagctgtcct ggggcagtac caccacctcc ccctgcggaa gcatatcaat cagcttcctg
    14581 actaccgttg tctttcctga tcctgtgcct ccggcgatcc cgacaataag cataaaatga
    14641 ttatttttgt atcaaaagta acaaaaatca ctttatggta aagcacatcg tgatcttcaa
    14701 gcttgcccag ccggtcactc cggaaaagag gaaccagtct gtgatgaagc tgaaggagat
    14761 tttcgggcct ctgggcaaca ggctgaagta cattgttgag tacaggacag gggtaaatat
    14821 ctccgatgtg gaccatgccg gggattttgt aattgatgcc acatttgcct cacttgatga
    14881 tctgaggcgg tatcagtcaa gtgagcttca ccgtgaggct gtggcggcag cttccattat
    14941 caaaaaggca aaactggtgg ttgactatac cttctgaagc tgatgagttc actttatccc
    15001 atccggttca agcccatgct cagggagttg ctctggggag gagacgccct gagggagcgg
    15061 ttcggcaaga aagcaaagcc gggcaccaaa atcggtgaga gctgggaaat atgcggaatg
    15121 ccgggaaaca gttcagtggt ggccaacgga ttcctgaaag gaaacagcct tgaagagatt
    15181 gcggagatct acatggatga gctgctgggt gaagcggtct tcgcaaagtt cggaacggag
    15241 ttcccgctgc ttgtaaagct cattgatgct gccgacaaac tctcgattca ggtccatccg
    15301 gatgacaggc ttgcttccga gaggcatcat gcctggggaa aaaccgagat gtggtatgta
    15361 attgatgcga aaccggggag tgtaatctac accggattca ggaaaaagac cacaagggaa
    15421 gagtatctcg aacacctgga agcaaaaaag cttgctgaac tgataaatgt tacacctgtc
    15481 agatccggtg atgtcttctt cattcctgcc ggattggtgc acgccatcgg agggggagtg
    15541 cttcttgcgg agatacagca gacatctgac gtcacctatc gcatctacga ctgggaccgt
    15601 gttgacacgt caggcaggcc gcgcgagatg catacctcac tggctctcga tgccataaat
    15661 ttcagctttg acagcaccaa tctgatcaga accagtccgg aactcaaccg gacagtgttg
    15721 ctggctgaaa gtcaatattt ccataccagc ctgataacct ttgacaggcc gataataaag
    15781 gattacagcc tgactgactc cttcatcatc tacatctgca cacagagcct ggcagtagtg
    15841 gagtgcaacg gccacagtga ggagataaag gctggtgaga ccattctcat tcctgcctcg
    15901 gctgacagtg ttgccataat cccccggggc acagccactc tgcttgaagt attcatacca
    15961 caaaaaaata gatcaaaatg aaacagctta cactacttac agcagcaatt atcatgattc
    16021 ttgcctccgc ctgcggagga ggtgccggcc aggaaggaag tggcaggaag aaggatgcgc
    16081 atgaggctga cacaggatat acaggcataa aaaactatat caaggataat ataaagctca
    16141 aggaggtcac atataaaaac ggtattcgtg aagggatgac ccgtaccttt tacaagggcg
    16201 gccagttgga gcaggagata ccatactccg gtgacagcaa gaacggagaa gccaaatggt
    16261 tctatactga cgggaagtta ttccggacaa ccccctttgt caatgatacc attcatggtg
    16321 accaggtgca gtactacaag aacggagggg taaaggcacg tctcagctat actgacggca
    16381 agcgtcaccc ctcgctggaa gagttcctga tgagcggaga gaaggtgacc gattatcctg
    16441 cactgcgtta cagggtcaat gaccgttacg cggaaagagg actctacaaa atattcgtcg
    16501 agttcgataa catggctgag aatggaaagt tttacagggg tgactacatc aacggcctgg
    16561 ttgaccttga ctccctgaac cccatgctgc agaccgtcac caccgggtat ctcgatttgc
    16621 agaaaagacc aggccacaaa gccgattcag tggtagtaat agcatcatac ctgacgcagt
    16681 ttggcaacag gctctattac cggcttgcaa tacccttacc ttataaagat ttaaactaaa
    16741 ctatatgtca cagattctag tgcctggcat caggctcaca acaacaagaa cggtcagcaa
    16801 aaatgatacg gccgcggttt acggatcagg tctgcatgat gtgctctcca cacctgccat
    16861 ggttgctttc atggaacaga cagcgatgca ggctgttgaa gcctgtctca acgagggaga
    16921 gggcaccgtg ggaacggagg tcaatatcag acaccttaaa gccacaccgg tgggcatgac
    16981 catcacctgt accgcaaccc ttcaggaggt aaacgggaac aggctcctct ttcatgtaga
    17041 ggcagacgac gagatcggca agattggcga aggacaacat gagaggttta ttatcaacaa
    17101 ccagagattc aaggaaaagc tgaatggata atgatcatca gatcagcctc ctttgtcacc
    17161 agtagtgcaa ggatcagtca gttgccgccg cctgaccttc cggaatatgc attcataggc
    17221 aggtcgaacg tcggcaagtc atcgctgata aatatgctgg tctcgagaaa agggttggca
    17281 aagacatcgg gaagcccggg caagacacag accatcaatc atttcattat caatgagagc
    17341 tggtacctgg ttgacctgcc cggatatggt tatgcacgcg tgtcacagaa gatgcgtgat
    17401 cagtgggaga agctcatcac atcatatatc actatgaggg agaatctcat cctgctcttc
    17461 gtgctgattg atggcaggca tgaaccgatg gcatctgacc tggcattcat ggagagactg
    17521 gcaatgaacg gaataccctt tgccagggtc ttcaccaaaa ccgacaagct gtcagctgct
    17581 gaagcagcga agatcaggga ggtccatgac agagtaatgc tgcgctcatg ggagagcctc
    17641 cctcctgcct tcgtaacatc tgcttccagg ggcacgggca gggaggcggt attagactat
    17701 atcgaaagca atatgatttt ctttaccggg atgaaatgat taacccataa atattaattt
    17761 tggggcatca tagagtgaaa ccaaaaacaa cttccttata tgatcagcag ggctccgttt
    17821 aagatttttt cctgtacctc ttcattgaca atagcagaaa agattg
//
LOCUS       DAOPBK010000033        17818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3259_length_17818_cov_4.400721, whole genome shotgun sequence.
ACCESSION   DAOPBK010000033 DAOPBK010000000
VERSION     DAOPBK010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17818
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3259_length_17818_cov_4.400721"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..602)
                     /locus_tag="PLA17_03265"
     CDS             complement(159..602)
                     /locus_tag="PLA17_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84462.1"
                     /translation="MRQLIQILMVTLFLSLLVPGEAAAQCKGFAKSLCKTELGDYLHD
                     GNYHAAVLVEGEEAELYKTFYSDLDYRLAICSADGMPPVEFIVYDATGKVLYNNKDEG
                     PAKTWDFRLESSQQLKVVVKVSTFGSREQLPVSGCVAIMFGFKAK"
     gene            complement(609..1529)
                     /locus_tag="PLA17_03270"
     CDS             complement(609..1529)
                     /locus_tag="PLA17_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84463.1"
                     /translation="MNSKLKILPLILGVTALAFCLVATSCKGKTRGDASRDKAEAVSP
                     DEQTMEQIKNAEKVFNALPTPLEVAQLMKEAGARFDATLLNPVENKSNYTSSKSMALN
                     LGTYVCDLSFASMYDETSLLVNYMEAAEDMATGLGILDAIDETTVERLEQNINNRDVI
                     MEIVSETFLNSQAYLEDDEQHAVAATIIAGGFIEGLYISTRLVDMKNYKGNKLVQTIA
                     DQKMSVDDMIRLLETYTDNTAIRELLGPIHDLKAIFDKIERSTTPSNAQYNEKIGMTE
                     ITGAAKSDMKQEVFLEIRDKVAEIRNSYVN"
     gene            1645..2160
                     /locus_tag="PLA17_03275"
     CDS             1645..2160
                     /locus_tag="PLA17_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84464.1"
                     /translation="MERSAKKLSELERILEKGTRREINDRIRLLRTEMPFGGALKMLA
                     MFYDKTGDHILRAAISDLFNDIKERSAMAEVIESISAVRKPDSRAMLISSCWQSGLDY
                     SPFAVTLAEYFVSGDYMTSLECFTVLDTCASSISHNDRARIIITMQEKKASWETPKQI
                     LAGELIALLKG"
     gene            complement(2188..3165)
                     /locus_tag="PLA17_03280"
     CDS             complement(2188..3165)
                     /locus_tag="PLA17_03280"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPK84465.1"
                     /translation="MSIINTRYLGFDLSSPIIVGSSNFTSRVESIVEAEAAGAGAVVL
                     KSLFEEQIVSQAHSLSSQESYPEADDYLKYYTRTNSVDTYLDLIAGARKKVKIPVIAS
                     INCFSPEGWIDFALEIEKAGASALEVNIFFVPTDKQKSSADSEKVYFRIIDNLKRKLK
                     IPVSVKIGPKFSNILNFVWQMHNHGAAGVTMFNRFYEPDIDINNMNIVPAPVLSNPLE
                     QRYVLRWIAMVSAQDINIEISASTGVYTGEDAIRYLLAGATTVQVCSALYKRGFAVVS
                     EINETLRRWMDNKEFKSISDFRGKLNYRNIDNPSLFERSQFMKHYSSYI"
     gene            complement(3172..3846)
                     /locus_tag="PLA17_03285"
     CDS             complement(3172..3846)
                     /locus_tag="PLA17_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPK84466.1"
                     /translation="MISNIAENIRKLLDELPPGVSVVAVTKTRTEEEILEAYRAGLRI
                     FGENRVQEMIAKKPLLPDDISWHFIGRLQSNKVKQTVAAASVIESVDSLRLLRLIDAS
                     ALAQGKCTECLLQVHIAAEETKSGFSLEDMEETDWRQVSDSLKAARICGVMGMATFTD
                     DMDKVRSEFRALYNLFGRIREKYFAGSSHFREISMGMSGDWPLAVSEGSTMIRIGTLI
                     FGERNN"
     gene            complement(3862..5112)
                     /locus_tag="PLA17_03290"
     CDS             complement(3862..5112)
                     /locus_tag="PLA17_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPK84467.1"
                     /translation="MAVVKPFRGLRPPANLAAEIACLPYDVMNSEEAAVMAKGKEKSL
                     LHITKAEIDLPEGTDVHSEEVYQKAFENFNLWQKKGWLAEDKERYFYIYAQTMEGRTQ
                     YGIVGAAAVNDYLSGKIKKHELTRPDKEEDRMTHVRANNANIEPVFYTYPAVEEIDKI
                     VEKIVKDEKPEYDFVAEDGIGHHFWVVWDRKTNERIEKLFAEKVPYMYVADGHHRTAA
                     AARVGKEKKEKNPAHRGDESYNYFMTVIFPDDQLKIFDYNRVVKDLNGMTPEQFLEKL
                     KESFDISEKGSEIHRPARLHNFGMYLDGKWYSLTAKEGTYSDDDPIGVLDVTILSEQV
                     LQPLLDIQDLRRSKRIDFIGGIRGLGELKKRVDSGEMKVAFALYPVSMKQLITIADTG
                     NIMPPKTTWFEPKLRSGLVIHKLD"
     gene            complement(5132..6058)
                     /locus_tag="PLA17_03295"
     CDS             complement(5132..6058)
                     /locus_tag="PLA17_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK84468.1"
                     /translation="MNILVATEKPFAPVAVSMIREVVEKAGHKLTILEKYKTPQELHA
                     AAADADAMIVRSDIVDSAVIAAAPKLKIVVRAGAGYDNLDLNACTAANVVAMNTPGQN
                     SNAVAELAFGMMVYGARGGFDGKAGSELMGKTLGLHAFGAVGRCMARIASGFGMKVCA
                     FDPFIDGKIIEEAGVTPVATIEDLYSGCQYVSLHIPANEQTKGSIGEKLLSLMPQGAV
                     LINTARKEVINEAELVAMMEKRGDLKYLSDVTPDNAELFAQRFAGRYFFTPKKMGAQT
                     AEANINAGVAAARQIVSFFATGDQTFRVNKPK"
     gene            complement(6075..7145)
                     /gene="serC"
                     /locus_tag="PLA17_03300"
     CDS             complement(6075..7145)
                     /gene="serC"
                     /locus_tag="PLA17_03300"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPK84469.1"
                     /translation="MKKHNFYAGPSIMPQYTNEKVIEAIRDFAGTGLSIMEVSHRSKE
                     FDAVMESAVTHVKELLDIPEGYSVIFLGGGASLQFAMLPMNLMEKKAAYINTGEWASR
                     AIKEAKLFGEVIEVASSKDRNFNYIPKNFDIPSDVDYFHITTNNTIFGTQWKKDPDVK
                     VPLVADMSSDIFSRPVDVSRYSVIYGGAQKNLAPAGVTFVIIKDSALGKVTRQLPTML
                     NYKTHIDKGSMFNTPPVLPVFAALKTLEWLKQKGGVAAMAKINQEKAALLYDEIERNK
                     LFKVTVPDPEDRSLMNVCFVMSDNYKELEKEFGDFAKSKGMVGIAGHRSVGGFRASLY
                     NALPIESVRALVECMKEFENKH"
     gene            complement(7228..7944)
                     /locus_tag="PLA17_03305"
     CDS             complement(7228..7944)
                     /locus_tag="PLA17_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPK84470.1"
                     /translation="MTQNSLEICFTPAAFEAHADSEAVVVIIDVLRATSSICTAFANG
                     VREIIPVETVEEARAMKARGYLVAAERDGYVLDFADFGNSPFNFTPEKVKGRSVVYST
                     TNGTGIMKRASVCHDVVIGSWLNISVLCNWIAEQGRKVVIVCAGWKKRFSLEDAVCAG
                     AMATRLLESGKFCTICDSVHAATDLWMMARTDLLGYMEKAAQRSRLRDKGLDDCIPYC
                     HKFDVTDVIPVLRGGRLTTL"
     gene            complement(7969..9078)
                     /gene="gcvT"
                     /locus_tag="PLA17_03310"
     CDS             complement(7969..9078)
                     /gene="gcvT"
                     /locus_tag="PLA17_03310"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPK84471.1"
                     /translation="MKNTPFLKYHEALGARIIPFAGYNMPVEYTGITEEHLTVRSGVG
                     VFDVSHMGEFWVNGPRALDFLQYITSNDVSALTDGKVQYACFPNDRGGIVDDFLVYRF
                     SAEKYLLVVNAANIEKDWEWCVKHAAAFGITAGPGGELNNASDGTAQLAVQGPMALKA
                     MQKLTDEPVTDMEYYTFKVLKFAGIDDVIFSITGYTGSGGCEIYIRNADAPRLWEEVF
                     RAGEEFGIKPVGLGARDTLRLEMGFCLYGNDINDTTTPIEAGLGWITRFVPDKDFIGR
                     DLLLKQKEEGPERRLKGFVMIDKGIPRQHYEVVDADGNQIGEVTSGTMAPSLKQGIGL
                     AYLNKGFWKSDTEIFIRVRGRDLKAKVTTLPFYKQ"
     gene            9229..9582
                     /locus_tag="PLA17_03315"
     CDS             9229..9582
                     /locus_tag="PLA17_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPK84472.1"
                     /translation="MEYRVFRPEYLLSITGGDMETMNEIAGIFGSQVPEFVNDMKSLL
                     GQEKYYELGLLAHKAKGSVTVLGMDETAKMLKEFELLAKAGEQKEKYSGFISRFEADT
                     SMVMAEVEDYLSKNR"
     gene            9595..9939
                     /locus_tag="PLA17_03320"
     CDS             9595..9939
                     /locus_tag="PLA17_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK84473.1"
                     /translation="MTDSTDGIEILSFRTDRINALNVDVIRPVILKLFETPYTKVVID
                     MAGVNYLDSTGFAMFLHLLRVARSNYCTYRLCSLTPGVLELFEMLRLHHSLDIYPDRE
                     TCLESYRQNSPY"
     gene            complement(9936..12527)
                     /gene="clpB"
                     /locus_tag="PLA17_03325"
     CDS             complement(9936..12527)
                     /gene="clpB"
                     /locus_tag="PLA17_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPK84474.1"
                     /translation="MNFENFTIKAQESVQKAVTIASGKGQQAIECGHLLKGILTEAES
                     IVSYIMSKTGGNPAGFGKALDAIIDGYPRVSGGEPYFSQNVAGVFRRAADHAAKMQDR
                     FVSVEHLLLALSESADSISQLMKDNGMVTSGLLKAIADLRKGAKVNSQTSEETYDSLN
                     RYAIDLNERARAGKLDPVIGRDEEIRRVLQILSRRTKNNPILVGEPGVGKTAIAEGIA
                     QRIVRGDIPEDLVRKRIFSLDMGALIAGAKYKGEFEERLKSVVNEVIASDGDVILFID
                     EIHTLVGAGKGEGAMDAANILKPALARGELRAIGATTLNEYQKYFETDKALERRFQTV
                     MIDEPDRADAISILRGLRERYETHHKVIIRDEAIISAVDLSYRYITDRFLPDKAIDLI
                     DEAAARLRLEMNSVPEEIDTADRQIRQLEIEREAVKRDGDTVKEKELSKEIADLAAGR
                     DELKARWRAEKDLVENLQKKKQEGEELRFEAERAERAGDYGRVAEIRYGKLTDNEKEI
                     EELNAEIAEKRNAGSMVRDDVRSDDIAEIVSRWTGIPVSRMLESEKEKLLRLEEELHR
                     RVVGQDEAIRAVSDAVRRSRAGLQDEKRPVGSFIFLGTTGVGKTELARALAEYLFNNE
                     NLMTRIDMTEYQERHSVSRLIGAPPGYIGYDEGGQLTEAIRHKPYSVVLLDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTVNFRNTIIIMTSNLGSSLIRESIEESSGEMTEEKAE
                     ELRADLVQMLRTTLRPEFVNRVDEIVMFRPLTLMQIREVVMMQFSRVAQMLEEKGISS
                     ELTEKAVAWLAERGFDPLFGARPVKRLIQKEIVNELSKKIIAGSIDKSRKVKIDVQSG
                     ELVITNA"
     gene            12688..15762
                     /locus_tag="PLA17_03330"
     CDS             12688..15762
                     /locus_tag="PLA17_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK84475.1"
                     /translation="MNEKILNTLMHLFAIIAPAQGNESDRRGVVEAFLTPQLNQEGVK
                     AYLKIFDAYYAEAQKRLKKGKEVRRNSSISVRIIKICYEIGTQLTLNQKIIVLVQLLE
                     YCRSDNKEVSNTELEFIITAAEGFNIDPEDYLLIRQFVLSTRDEVPESSQILIIDSDR
                     VNRYEKTHHIVNDSFRGQVRVLNLPQADMLFIRAFGTGELLLSGQLRHEDRVYLFERG
                     ASLKFMSSKPIYYSEVIRQFLDESALASRVVYEVNDIEYKFKGGQVGLHRMSFAEESG
                     RLIGIMGSSGAGKSTLLNVLNGINRPDSGEVLINGINIHDEPDKIKGLIGFVSQDDLL
                     IEELTVFENLFYNAKLCFDNLDDEAITSKVDDVLHSLGLFEIKDMKVGTPLNKKISGG
                     QRKRLNISLELIREPAIMFLDEPTSGLSSLDSENILDLLNDLKLKGKLIFVVIHQPSS
                     DIFKMFDRLIFLDSGGYMIYYGIPVRAIDYFKDKVQLPRYNDSECHACGNVNPEQIFS
                     IVESKVLDEFGRPTMTRKVSPAEWSAYFNSRREELPPHPVAGNGIPEITLRTPGRLKQ
                     MAVFVKRDILAKLSDSQYLIITLLEAPVLALFLAFIIRYFDESVSQPVYTLLQNDNLP
                     VYIFMAVIVAIFMGLTVSAEEIIKDRKILKREAFLNLSWNSYLLSKICVQFLISAIQA
                     ASFVAVGNTVFGIRGMWFEYWLVLFSCWATSNMMGLLISDSFKTVVTIYILIPFLVIP
                     QIILSGIIVKYDKLNPTISSPVSIPLYGEVFAARWGYEALAVDQFINNRFEKQFYGYD
                     KVISTAKFRKDIWYNEMKSILSRLESNLERERLDKSSVNDMMLIRNEIEEELGFTAAV
                     TFDPGIINPDSITAEALAAARDYIERVRRYYIEVSRNAVEARDKVIIAFEQADREAFI
                     RMKKQHTNESLEEFVRNDNEKDKIKRYRDRLYQNYDQIFFDPTHPLVKAHFYAPQKQV
                     FGLFFGTLAVNTAVIWFMTICLYILLYFRVLHHILDSSEKFIRVMRTKRERG"
     gene            complement(15975..16355)
                     /locus_tag="PLA17_03335"
     CDS             complement(15975..16355)
                     /locus_tag="PLA17_03335"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPK84476.1"
                     /translation="MEPIIIEGTPKTPSIKFDTRDGVFEIKGRSIPENSVEFYKPLNE
                     WLDQYMQTPLDKTVVNIRLEYFNTSSSKCILDVFKRLEAIHRSKHDVVINWFYEEDDE
                     DMLEAGEDYDSIIKVPFKMIEIVE"
     gene            complement(16381..16935)
                     /locus_tag="PLA17_03340"
     CDS             complement(16381..16935)
                     /locus_tag="PLA17_03340"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HPK84477.1"
                     /translation="MSFDLENLVSDLDIRGAVLYYKGNVESDTITKALDLVEAKLFAT
                     REAPRLRKKVYNVLVESLQNLYHHSDRVPRGYHAARPDRFGLVVMEKTERGYRITTCN
                     FVAIVRVKELEEKLTRINRSTPEEIKEQYKDVLNNQEITEKGLGGLGLLDIARKTGHR
                     IDFRFRQYDSLNTAFRLTAVVAQV"
     gene            complement(17078..>17818)
                     /locus_tag="PLA17_03345"
     CDS             complement(17078..>17818)
                     /locus_tag="PLA17_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPK84478.1"
                     /translation="VNVHISNNRVTISIDSTVVPLYRRGYRRRMSEAPLSEVLAAGLI
                     ALSGWKPGTPLTDGMCGSGTIVTEAGLISCNIAPGRMRKNFGFMRWKDYDAALFRSVR
                     FDAEKRERKSPAAIFGSDISAEACAIARANVRAAGLEDTVEISGRDFFESPPPAENGM
                     LIINPPYGRRLGTSDMDEFYGRIGSVLKHRYEGYTAWILSGDMDSLRKVALKPFRKFD
                     LLNGDIECRFQGYELYGGTRKHKNVSGA"
BASE COUNT         4338 a         4690 c         4043 g         4747 t
ORIGIN      
        1 aaaaggatca tattgctttc ctgtgaattc tttgacagtg ggtttatgaa aaaaaaggag
       61 cgcggctata tgtagcgctc cggtacctgt gcagataaaa ctgaccttaa gggggcaact
      121 cactctgccc atcagccggt caattcatcc gggcaatgct attttgcctt gaaaccgaac
      181 atgattgcaa cgcaaccact gaccggcaat tgttctcttg aaccgaaggt tgatactttt
      241 actaccactt tcagttgctg gcttgactca agcctgaaat cccatgtctt ggccgggccc
      301 tcatccttat tgttgtagag cactttaccg gtggcatcat aaactatgaa ctccaccgga
      361 ggcatgccat cagccgaaca aattgcaagc cggtaatcca ggtcggagta gaaggtcttg
      421 tagagttcag cctcctcacc ctcaaccagg acggctgcat ggtagttacc gtcatgaagg
      481 tagtcaccca gttccgtctt gcagagtgat ttggcaaatc ctttgcactg cgcggcagct
      541 tccccgggaa caagtagcga gaggaagagc gtaaccatta aaatctgtat aagctgtctc
      601 atcggtatct agtttacgta tgaattcctg atctctgcca ccttatccct gatttccagg
      661 aaaacctcct gcttcatgtc tgattttgcc gcaccggtga tctccgtcat gccgatcttt
      721 tcattgtact gcgcatttga gggtgtggtt gaccgttcaa tcttgtcaaa gatggctttc
      781 agatcgtgta tgggcccgag gagttctcgt atagcggtat tgtcagtata tgtttccaga
      841 aggcgtatca tatcatcgac tgacatcttc tggtcagcga tggtttgtac cagtttgttg
      901 cccttatagt ttttcatatc aaccagcctg gttgatatat aaagcccttc tataaagcct
      961 cccgcaataa ttgtagcagc cacggcatgc tgttcatcat cctcaagata agcctgtgaa
     1021 ttgaggaatg tctccgagac tatctccatg ataacatctc ggttattgat attctgctca
     1081 agccgctcaa cggtggtctc gtcgatggca tcaagaatac ccaggcctgt ggccatgtcc
     1141 tcagccgcct ccatataatt gaccagcaag gaggtctcat catacatgct cgcaaaactg
     1201 aggtcacaaa catatgtgcc cagattaagc gccatgctct tgctggaggt atagttgctc
     1261 ttgttctcaa caggattcag cagtgtagca tcaaacctcg cccctgcttc cttcatcagc
     1321 tgagccacct cgagcggtgt aggcaacgca ttgaatacct tctcggcatt ctttatctgc
     1381 tccatcgtct gctcatcagg actgacggcc tccgccttat ccctggatgc atcacccctg
     1441 gtttttccct tgcatgaggt ggcaaccagg cagaatgcca gagcggttac acccagaatc
     1501 agtggcagaa tcttaagctt actgttcata gtatttgtag tttatgattt cacaggcctt
     1561 ctccagacca atttacactt ttttaataaa cgaatttgcc atgaacttat tatttagcaa
     1621 taaatttgtt ccaaagcaaa ccggatggaa agatcagcaa agaaactgtc agaactggaa
     1681 aggatactcg aaaagggaac ccgaagagag ataaatgacc gtatcaggct gctccgcacc
     1741 gaaatgcctt tcgggggtgc actgaagatg cttgcaatgt tttatgacaa gaccggagat
     1801 catatcctca gggccgcaat atcagacctg ttcaatgata taaaggaaag atcggccatg
     1861 gccgaagtga ttgaatcgat ctctgcagta agaaaaccgg attccagggc aatgctcatc
     1921 tcgtcatgct ggcagtcagg acttgattat tccccgtttg cagtgacact tgccgaatat
     1981 tttgtttcag gtgattatat gacctctctc gaatgcttca cggtactcga cacctgtgcc
     2041 tcatcaataa gccacaatga ccgcgccaga ataataatta ccatgcagga gaagaaggcg
     2101 tcatgggaaa caccaaagca gatacttgcc ggtgagctca tagccttgct gaaaggctga
     2161 agcccgggca acctgcagaa cagctttcta tatgtaggag ctgtaatgct tcataaactg
     2221 cgaccgctca aacagggatg gattgtcaat attcctgtaa ttaagctttc ccctgaagtc
     2281 gcttatgctt ttgaactcct tgttgtccat ccacctgcgc agagtctcat tgatctctga
     2341 cacaaccgca aaccccctct tgtatagggc agagcagacc tgtactgtag tagcgccggc
     2401 cagcaggtac ctgatcgcat cctcaccggt atatacaccc gtggaggcag agatttcaat
     2461 attgatatcc tgggcactga ccatagctat ccaacgaagc acgtaccgct gttcaagagg
     2521 attggaaagc accggggcag gcacaatgtt catattgttg atatcaatgt caggctcgta
     2581 gaacctgttg aacatagtca cccccgcagc tccgtggttg tgcatctgcc acacgaagtt
     2641 caatatgttt gaaaacttcg ggccgatttt gacgctgacc ggaatcttaa gctttctctt
     2701 aaggttgtca attatcctga agtaaacctt ctccgaatca gcactgcttt tctgcttgtc
     2761 agtgggtacg aagaagatat tcacctcaag cgcactggcg cctgccttct ctatctcaag
     2821 tgcaaagtca atccatccct cgggtgagaa gcagttgatg cttgctataa caggtatctt
     2881 caccttcttg cgtgctccgg ctatcaggtc aagataagtg tcaacactgt tggtacgggt
     2941 atagtactta aggtaatcgt ctgcttcggg gtatgattcc tgtgacgaca atgaatgggc
     3001 ctgtgaaact atctgttcct caaacaggga tttcaggaca actgctccgg ctccggctgc
     3061 ctcagcctca acgattgatt ccaccctgga ggtgaaattg gaactgccca cgataatggg
     3121 actgctgagg tcgaatccca ggtagcgtgt gttgattatg ctcatcttgc tttaattatt
     3181 tcgttctcca aatataagtg ttcctattcg aatcatggta cttccctcac tgacagccag
     3241 gggccagtca cccgacattc ccatcgagat ctccctgaag tggctgctgc cggcgaaata
     3301 cttctcacga atcctgccga aaagattgta aagggccctg aactctgacc ggaccttatc
     3361 catgtcatcg gtgaatgttg ccatccccat cactccgcat atccgtgctg ccttcagcga
     3421 gtcagatacc tgacgccagt cagtctcttc catatcctcc agtgagaaac ctgacttggt
     3481 ctcttccgca gcgatatgaa cctgcagaag acactctgtg catttcccct gcgccagtgc
     3541 gcttgcatct atcagccgca gaagcctgag tgaatcaacc gactcaatta cagaggctgc
     3601 tgcaactgtc tgtttcacct tgtttgactg caggcgacct atgaaatgcc atgaaatatc
     3661 atcaggaagc aagggctttt ttgctatcat ctcctgcacc ctgttctcac caaagatgcg
     3721 gagtcctgcc ctgtatgcct caagaatctc ttcctctgtc cgtgttttgg ttactgcaac
     3781 cacgctgacg cctggaggca actcatcaag cagcttcctg atgttttctg caatattgct
     3841 aatcatggca ccggactgcc atcagtcaag tttatgaatc accaggccgc ttctgagctt
     3901 gggttcgaac catgtggttt ttggaggcat gatgttcccg gtatcagcta tggtaatcag
     3961 ctgtttcatg gatacgggat agagtgcaaa ggccaccttc atttcaccgc tgtcaaccct
     4021 tttcttcagc tcgccgagac ccctgatgcc accgataaaa tctatccgct tcgaacgccg
     4081 cagatcctgt atgtcaagca gaggctgcaa aacctgttcg ctaaggatag tcacatcaag
     4141 aaccccgatg ggatcatcat cagaatatgt accctctttg gccgtcaggc tataccattt
     4201 gccatcaaga tacattccga aattgtgaag cctggcaggc ctgtgaatct cacttccctt
     4261 ctcactgata tcaaatgatt ccttcagctt ttcgagaaac tgttcgggtg tcataccatt
     4321 gaggtccttc acaaccctgt tgtaatcgaa gatcttgagc tggtcatcag ggaagattac
     4381 tgtcatgaaa tagttgtacg actcatcgcc cctgtgggcc ggattcttct ctttcttttc
     4441 cttaccgaca cgcgctgcag ctgctgtacg gtggtgacca tcagcaacat acatataagg
     4501 aaccttttca gcaaaaagct tttcgattct ttcgttggtc tttctgtccc atactaccca
     4561 gaagtgatgg ccaatgccgt cttcagcaac gaagtcgtac tcgggtttct catccttcac
     4621 gatcttttca acaattttgt caatctcctc aacagcagga taggtataga acaccggttc
     4681 aatgttggca ttgtttgccc ggacgtgggt catgcggtct tcctccttat caggacgggt
     4741 cagctcgtgc tttttgatct tgcctgaaag ataatcattc actgctgcgg cgcccacgat
     4801 gccgtattgc gtacggcctt ccatggtctg ggcatagatg tagaaatatc tttccttgtc
     4861 ttctgccagc cacccctttt tctgccatag gttaaaattt tcaaaggcct tctgatatac
     4921 ctcctccgag tgcacgtcag tgccttcggg caggtctatc tcggctttgg tgatgtgtag
     4981 cagtgatttt tccttaccct ttgccatcac ggctgcctct tcggagttca tcacgtcata
     5041 gggaaggcag gcaatctctg ctgcaagatt ggccgggggc ctgagccccc ggaaaggttt
     5101 aacaacagcc atatctgaaa tttatctctt actatttggg tttattaact ctgaaagtct
     5161 ggtcacctgt tgcaaagaat gaaactatct gacgtgcagc agccactccg gcattaatgt
     5221 ttgcctctgc tgtctgggct cccattttct ttggtgtaaa gaaatagcgg ccggcgaacc
     5281 tctgtgcaaa cagctctgca ttatcagggg ttacatcaga aagatacttc aggtcacctc
     5341 tcttttccat catggcgacc agttctgcct cattgataac ctccttgcgg gcggtgttaa
     5401 taagcactgc tccctgtggc atcagtgaca gaagcttttc tcctatggag ccctttgtct
     5461 gttcatttgc aggaatatgc aatgaaacat actggcagcc agaataaagg tcttcaatgg
     5521 tggccaccgg ggtgacaccg gcttcctcaa taatcttgcc gtcaataaag gggtcaaaag
     5581 cacaaacctt catcccgaat cctgatgcaa tgcgggccat gcacctgcct acagcaccga
     5641 atgcatggag gcccagggtc ttgcccatca gttcggagcc cgctttgccg tcgaagcctc
     5701 cgcgtgcgcc atagaccatc atcccgaatg caagctctgc aacagcattt gaattctgcc
     5761 cgggagtgtt catggcaacc acgttggctg ccgtgcaggc attcaggtca agattgtcat
     5821 aacctgctcc ggccctgaca actatcttaa gtttaggtgc agcggcaatc accgctgaat
     5881 caacaatatc cgagcggact atcattgcat cagcatcagc tgctgctgca tgaagctcct
     5941 gaggggtctt gtacttctca aggatggtga gtttgtgacc tgccttctcc accacctcac
     6001 ggatcatgga taccgcaacg ggtgcaaatg gtttttcagt tgctacaaga atattcatat
     6061 cgtcctcctg ttttttagtg tttgttttca aattccttca tgcactctac caatgccctt
     6121 acactctcaa tcgggagagc attgtagaga gacgcacgga agcctcccac agagcggtga
     6181 ccggcaatgc ccaccatacc cttcgacttg gcaaaatcgc caaactcctt ctcgagctcc
     6241 ttgtagttgt cagacatgac gaaacagacg ttcatcagcg acctgtcctc cggatcgggt
     6301 acagttacct taaacagctt gttgcgctcg atctcatcat atagcagtgc tgccttctcc
     6361 tggttgatct tcgccattgc tgccactcca cccttctgct tcagccactc gagggttttc
     6421 agggctgcaa atacaggaag taccggaggt gtgttgaaca ttgaaccctt atcgatatgg
     6481 gtcttgtagt tcagcatcgt gggtaactgg cgggtaacct tgccaagggc agaatcctta
     6541 atgatgacga aggttacgcc ggccggtgca aggttcttct gtgcaccgcc atagatgacc
     6601 gaatacctgg aaacatcaac cggacggctg aagatatccg atgacatgtc agccacgagt
     6661 ggcaccttta catccggatc cttcttccac tgcgtaccaa aaatggtgtt attggttgta
     6721 atatggaaat agtcaacgtc tgagggaata tcaaaattct ttggtatgta gttgaagttg
     6781 cgatcttttg atgatgccac ctcaataacc tctccgaaaa gtttggcttc cttaatagct
     6841 ctgcttgccc actcaccggt atttatgtag gcagccttct tctccatcag gttcatcgga
     6901 agcatcgcaa actgcaggct ggcgcctcct ccgaggaaga taactgaata accttcaggt
     6961 atgtcaagca gttccttgac gtgagtgaca gcactctcca tgacagcatc aaactccttg
     7021 ctgcggtgtg agacctccat aatggaaagg ccggttcctg caaaatccct gatagcttca
     7081 atcacctttt cattggtgta ctggggcatg attgaaggcc ctgcgtagaa attgtgtttt
     7141 ttcatttcgt gattttttat ttgttcttgt aattcatttc aatctgttta tgccgccgga
     7201 tgtgaccgac tgcggcaatg taaatgctca gagggtagtc agcctgccac ccctgagcac
     7261 cggtatcaca tctgttacat caaatttatg acaataagga atacaatcat ccagcccctt
     7321 gtctctgagt ctggatctct gggctgcctt ctccatataa ccaaggagat cagtccttgc
     7381 catcatccag aggtcggtgg cggcatgcac tgagtcacat atagtgcaga acttcccgct
     7441 ttccaggagc cttgtggcca tagcccctgc acataccgca tcttcgaggc tgaagcgttt
     7501 tttccatccg gcacatacaa tcaccacttt acgtccctgt tcagcaatcc agttgcagag
     7561 gacagagatg ttgagccatg aacctatgac cacgtcatgg cagacagaag cacgtttcat
     7621 gatgccggtg ccattggtag tgctgtagac aacgctcctg cccttcacct tttcaggagt
     7681 gaagttgaac ggagagttgc cgaaatcggc aaagtcaagc acgtatccat cgcgctcggc
     7741 ggcaaccagg tacccccggg ctttcatcgc ccttgcctct tcaaccgtct caacgggtat
     7801 tatctcccgg actccattgg cgaatgcggt acagatggaa cttgtagccc ggagcacatc
     7861 tattatgacc actacagcct cgctgtcagc atgcgcttca aaggctgcgg gggtaaaaca
     7921 tatttccagg ctgttctgtg tcatggatgc aataataccg gttaatattc actgtttgta
     7981 aaatggcagg gtcgtgacct ttgccttgag gtctctgccc ctgacacgga tgaatatttc
     8041 ggtatcactc ttccagaagc ccttgttcag atatgccagg cctatcccct gcttcagtga
     8101 gggagccatg gttcccgagg tgacctctcc tatctggttg ccatctgcat caaccacctc
     8161 ataatgctgc ctgggaattc ccttgtcaat catgacaaaa cccttgaggc gtctttcggg
     8221 tccctcttcc ttctgtttca ggagcagatc cctgccaatg aaatccttgt ccggaacgaa
     8281 cctggtaatc catcccagac ctgcttctat tggagtagtg gtatcattga tatcatttcc
     8341 gtaaaggcag aatcccatct caagacggag agtatccctg gcgcccagac cgacaggctt
     8401 tattccaaac tcttcacctg ccctgaaaac ctcttcccag agacgtgggg catcggcatt
     8461 cctgatatat atctcgcacc cgcccgagcc tgtatatcct gtaattgaga agataacatc
     8521 atctattccg gcaaacttca gcaccttgaa ggtgtaatat tccatgtcgg tgacaggctc
     8581 atcggtcagc ttctgcatgg ctttgagcgc catcggaccc tgcactgcca gctgggctgt
     8641 tccgtccgag gcattgttca gttccccgcc cggtcctgcg gtgatgccga aagcggctgc
     8701 atgctttaca caccattccc agtccttctc aatgttggcc gcattgacca caagaagata
     8761 cttctcagcg ctgaaccggt aaacgagaaa atcatccaca atgcctcccc tgtcattcgg
     8821 aaaacaggcg tactgtacct tgccgtcagt cagggcagaa acatcatttg aggtgatata
     8881 ctgaaggaaa tcaagggcgc gcggaccatt gacccagaat tcacccatat gcgaaacatc
     8941 aaatacgcca accccggaac gtaccgtgag atgctcttcc gtaatccccg tatattctac
     9001 aggcatattg tagcctgcaa aaggaattat cctggcaccg agtgcctcat gatactttag
     9061 aaaaggagta tttttcattt gattaaactt ttgattaagt ggcaaaaata ggcattttcg
     9121 gacgaaaaaa agctgctata tatcattttt gacaccggtt cttcaggtcg ttcaaatgct
     9181 gtactggttt tataatgact atatttgtaa tctcaaaaca gcgaagaaat ggaatataga
     9241 gtattcaggc ccgaatacct gttgagcatc acgggaggtg atatggagac catgaatgag
     9301 attgccggga tattcggcag ccaggtgccg gaattcgtaa atgacatgaa gagcctgctc
     9361 ggtcaggaga agtattacga actgggtctg ctcgctcata aggccaaagg atcggtgacc
     9421 gttcttggca tggatgaaac cgccaaaatg ctcaaggagt ttgaattgct tgcaaaagcc
     9481 ggggagcaga aggagaaata cagcgggttt atatccaggt ttgaagctga tacaagtatg
     9541 gtcatggccg aggtagaaga ctacctctcc aaaaacaggt aattgtttat ggtggtgacc
     9601 gacagtacag acggtattga gatcctgagt ttcaggaccg accgtatcaa tgctctcaac
     9661 gtggatgtga taaggcccgt tatcctgaaa ctctttgaga ctccgtatac caaagttgtc
     9721 atagacatgg caggtgtcaa ctatcttgac agcacaggct ttgccatgtt cctccacctt
     9781 ctcagggtgg cacggtcaaa ttactgcacc tacaggcttt gctcacttac cccgggggta
     9841 ctggagcttt tcgaaatgct ccgtttgcac cattcgctgg atatttaccc tgaccgggag
     9901 acctgcctgg aaagttatcg tcagaactcg ccctattagg cgtttgtaat caccagttcg
     9961 cctgactgca catctatctt tacttttctg cttttatcaa tggagccggc aatgatcttt
    10021 ttggagagct cattgactat ttccttttga atcagccgtt tcacgggccg tgccccgaat
    10081 agcggatcaa agcccctctc tgccagccag gccactgcct tctccgtaag ctcactgctt
    10141 atacccttct cctcaagcat ctgtgcaacg cggctgaact gcatcattac cacctccctt
    10201 atctgcataa gggtcagtgg cctgaacatc actatctcat caaccctgtt gacaaactct
    10261 ggtctgaggg ttgtgcgaag catctgtacc agatcggccc tgagctcctc agccttctcc
    10321 tctgtcattt caccgctgct ctcttcaatg ctctcacgga taagcgatga gccaaggttg
    10381 gatgtcatga taataatcgt gttacggaag tttacagtcc ttcccttatt gtctgtcagc
    10441 ctgccgtcgt caagcacctg gagcagtatg ttgaacacat cggggtgggc cttttcgatc
    10501 tcatcaagca gcaccaccga atatggcttg tgccttatgg cttccgtgag ctggccaccc
    10561 tcgtcgtaac caatgtatcc cggaggtgcc cctataagcc ttgatacgct gtggcgctcc
    10621 tggtattcgg tcatgtcaat gcgcgtcatc aggttctcat tgttgaacag atattcagcc
    10681 agggccctag ccagttcggt cttgccgaca ccagtggtac cgagaaatat gaatgagccc
    10741 accggcctct tctcatcctg cagtccggct cttgaacggc gcacagcatc cgagactgcc
    10801 cttatggcct catcctggcc aacaaccctg cggtgaagtt cctcctccag tctcagtagc
    10861 ttctccttct cactctcaag catccgcgaa acgggtatgc ctgtccacct tgacacgatc
    10921 tcggcaatat catctgaccg cacatcgtca cgtaccattg agccggcatt ccgcttttca
    10981 gctatctccg cattcagttc ctcaatctcc ttttcattgt cagtgagttt gccatagcgt
    11041 atctcggcaa cccttccgta gtcacctgcc ctttcagctc tctcagcctc gaacctgagc
    11101 tcctctcctt cctgtttctt tttctgcagg ttttccacca ggtccttttc agcgcgccag
    11161 cgtgccttga gctcatcgcg ccctgcagcc agatcagcaa tctcttttga cagctccttc
    11221 tccttgactg tgtcgccatc acgcttcact gcctcccgtt ctatctcaag ctgcctgatc
    11281 tgcctgtctg ccgtatcaat ctcctcaggc accgagttca tctcgagcct cagccttgct
    11341 gccgcctcat caatgaggtc aatggccttg tccggcagaa acctgtctgt gatgtaacga
    11401 tatgagaggt ccacggcaga gatgatagcc tcatccctga tgatcacctt atggtgcgtt
    11461 tcatacctct ccctgagccc cctgaggatg gagatggcat ctgccctgtc aggctcatct
    11521 atcatcactg tctggaatct cctttcgagg gctttgtctg tttcaaaata cttctggtat
    11581 tcattcagcg ttgttgcgcc tatggcgcga agctctcccc tggcaagcgc cggtttgagg
    11641 atgttggctg cgtccattgc accctcgcct ttgcccgcac cgacgagagt atgtatctca
    11701 tcaatgaaca ggattacgtc accgtcagat gcaataacct catttacaac tgacttgagc
    11761 ctttcttcaa attcaccctt gtactttgca cccgcgatga gcgctcccat atcaagcgaa
    11821 aatatccgct tcctgacaag gtcctcaggg atgtctccgc gcacaatcct ttgcgcaata
    11881 ccctccgcga tggctgtctt gcccacgcca ggctcaccta caaggatggg gttgtttttg
    11941 gtcctgcgtg acagtatctg cagcaccctc cttatctcct cgtcacggcc aatgacaggg
    12001 tccagcttcc cggccctggc acgctcattc aggtctatgg catacctgtt caatgaatca
    12061 tatgtctcct ctgatgtctg gctgttcacc ttcgcgccct tgcgcagatc agctatagcc
    12121 ttcaagagac ctgaagtaac catgccgtta tccttcatga gttgcgagat gctgtcagca
    12181 gattcggaga gtgccagcag caggtgctca acagatacaa acctgtcctg catctttgcg
    12241 gcatgatccg cagcacgcct gaataccccg gcgacattct gagaaaaata tggttcgccc
    12301 ccgcttaccc tggggtagcc gtcaatgatg gcatcaagag ccttgccgaa acctgccgga
    12361 tttccaccgg ttttggacat gatatatgag acaatgctct cagcctcagt caatattcct
    12421 ttaagcagat ggccacactc aatagcctgc tgacccttgc ccgaggcaat ggtcacagcc
    12481 ttctgtacag actcctgtgc ttttattgta aaattttcga agttcatagt tttactcttt
    12541 ttacctgcac tgttcaattt atttgccaat tgcctgaagg gactcaattc atgccattat
    12601 gacagacaag agataatata gaaggtgcca tgccgttata taatcacgat tcaggagacc
    12661 aaatgccgtt attgaaacct ctcagggatg aatgagaaaa tacttaatac cctgatgcat
    12721 ctctttgcca tcattgcccc ggcacagggt aatgagagcg acaggcgggg agtggtagag
    12781 gcatttctca caccccagct caaccaggag ggggtaaaag catacctgaa aatctttgat
    12841 gcatactatg ccgaggccca gaagagactg aagaagggca aggaagtccg ccgaaactca
    12901 tcgatatcag taaggattat caagatctgc tatgagatcg gcacacagct gaccctcaat
    12961 cagaagatca tcgtgctggt acagttactc gaatactgcc ggtctgacaa taaggaggta
    13021 agcaacaccg aactcgaatt cattattact gctgcagaag gcttcaatat cgatccggag
    13081 gattacctgc tgatccggca attcgttctg tcaacaaggg atgaggttcc tgaatcatct
    13141 cagatattga ttattgacag tgacagggtc aaccgatatg agaagaccca ccacattgtc
    13201 aacgactcat tcaggggcca ggtcagggta ctgaacctgc cgcaggctga catgctcttc
    13261 atcagggcat tcggcaccgg ggaactgctc ctgagcggcc agctgagaca tgaagacagg
    13321 gtctacctct ttgagagggg cgcctctctt aagttcatga gcagcaaacc tatctattac
    13381 tcagaggtca taaggcaatt cctcgacgaa agcgccctgg cttcacgggt ggtctacgag
    13441 gtaaatgata tcgaatacaa gttcaagggc ggacaggtag gtctgcaccg catgagcttc
    13501 gcggaagagt caggaaggct tatcgggatc atgggatcat ccggggctgg caaatcaacc
    13561 ctcctgaacg tccttaacgg catcaacaga cctgactcgg gcgaagtgct tatcaacggc
    13621 atcaacatac acgatgaacc ggacaagatc aagggactga tagggtttgt gtcacaggat
    13681 gacctcctga ttgaagagct gactgtattt gagaacctgt tctacaatgc aaagctctgt
    13741 ttcgacaacc ttgatgatga ggctatcacc agcaaggtgg atgatgtact gcacagcctt
    13801 ggcctgtttg agattaagga tatgaaggtg ggaactccgc tcaataagaa aatttcaggt
    13861 ggtcagcgca aacggctcaa tatttcactt gagctgatca gggaacctgc aataatgttc
    13921 cttgatgaac ccacatcagg tctctcttca ctcgactcag aaaatattct tgaccttctc
    13981 aatgatctga aactcaaggg caaactgata ttcgtcgtca tccaccaacc ctcttcagac
    14041 atcttcaaga tgtttgacag gctcatcttc ctcgattctg gcggatacat gatctattat
    14101 ggcatcccgg taagggccat tgactacttc aaggacaagg tacagcttcc gcgatacaat
    14161 gacagtgagt gtcatgcatg cgggaatgtt aacccggaac agatcttcag tatcgtggag
    14221 tcaaaggtgc ttgacgaatt cgggaggccg acgatgacga gaaaggtatc cccggccgaa
    14281 tggagcgcct atttcaattc ccgtcgtgaa gagctaccac cgcatccggt ggccggcaac
    14341 ggcatacctg agataacgct gcgcacaccg ggcaggctta agcagatggc ggtatttgtc
    14401 aaacgtgaca tactcgccaa actctctgac tcgcaatacc tcattattac ccttctcgaa
    14461 gcgccggtac tggccctgtt cctggcattc attataaggt attttgatga aagcgtaagc
    14521 cagcccgtct acaccctgct tcagaatgac aacctcccgg tttatatctt catggcagtg
    14581 atagtggcaa tattcatggg cctgacagtc agcgcggagg agatcatcaa ggacaggaaa
    14641 atcctcaagc gggaagcttt ccttaatctc agctggaaca gctacctgct ctcaaaaata
    14701 tgtgttcagt ttctgatctc tgcaatccag gccgcatcat tcgttgccgt tggaaataca
    14761 gtattcggaa tacgcggtat gtggttcgaa tattggctgg tgctcttctc atgctgggca
    14821 acgtccaaca tgatgggact gctgatctct gacagtttca agacggtagt tacgatttac
    14881 atcctgattc cgttcctcgt tatacctcag atcatactga gcggaataat tgtgaagtat
    14941 gacaagctta atcccactat ctcgtcacct gtctcgatac cgctttacgg tgaggtcttt
    15001 gcagccaggt ggggttatga ggcgctggcc gtggaccagt ttataaacaa caggtttgaa
    15061 aagcagttct acggttatga caaggtcatc agcactgcca agttcaggaa agacatctgg
    15121 tataatgaga tgaaaagcat cctctcaagg ctggagagca accttgagag agagaggctt
    15181 gataaatcat cagtaaatga catgatgctc atcaggaacg agatcgaaga ggagctcggt
    15241 tttactgcag ccgtaacgtt tgacccgggc atcataaatc cggattccat caccgctgag
    15301 gcactcgctg cggcacgcga ttacatagag agagtcaggc gctattatat tgaagtgtcg
    15361 cggaacgctg tcgaagcgcg tgacaaggtt atcattgcct ttgagcaggc tgacagggaa
    15421 gcatttatcc ggatgaagaa acagcacacc aatgaatctc tcgaggagtt tgtaaggaac
    15481 gacaatgaaa aggacaagat aaagcgatac cgtgacaggc tctaccagaa ctatgaccag
    15541 atattctttg atccgacgca cccgttggta aaggcgcact tctatgcgcc gcagaaacag
    15601 gtgttcgggc tcttttttgg cacgctggca gtcaatacgg cagtcatctg gtttatgacc
    15661 atctgtcttt acatcctgct ctacttcagg gttctgcatc acatccttga ttcttcggaa
    15721 aagttcatca gggtgatgcg cacgaagcgc gaaagaggct aaagtttata aaagcgatgc
    15781 gtacgaagcg caaatgaggc tgaagtttat aagaacgatg cgcacgaagc ccgaatgaag
    15841 ctgaagatta taagagtgat gcgcacgaag cgcaaatgag gctgaaggag ataatatata
    15901 aggggcatct gtctgatgac agataccccc ccctttcaat atcgtaaccg atctgattct
    15961 gtgccgaagc cgggttactc gactatctca atcatcttga aaggcacctt gatgatggaa
    16021 tcatagtcct cacctgcctc cagcatatcc tcatcgtctt cctcgtagaa ccagttgatt
    16081 accacgtcat gttttgacct gtgaatggct tccaggcgct tgaagacgtc aagtatgcat
    16141 tttgaggagc tggtattgaa atattcaagg cggatgttga caaccgtttt gtcaagcgga
    16201 gtctgcatgt attggtcaag ccattcattc agtggcttgt agaattccac cgagttctcc
    16261 ggtatcgacc tccccttaat ttcaaataca ccatcccttg tatcaaactt tatcgacggt
    16321 gtcttcggtg ttccttcaat aatgatcggt tccataacta tctttttttt gttaattttt
    16381 ctatacctgt gccaccactg cagtgagccg gaacgcagta ttcagactat cgtattgcct
    16441 gaacctgaaa tcaatgcgat ggcctgtctt cctggctata tcaagtaacc ccagtccgcc
    16501 aagccccttc tccgttatct cctgattatt aaggacatcc ttatactgtt ccttaatctc
    16561 ttcaggagta gaacggttta tcctggtcag tttctcctca agctccttaa ctctcacaat
    16621 ggctacaaaa ttgcatgttg tgatgcgata acccctttcg gttttttcca tcaccaccaa
    16681 cccaaacctg tcaggcctgg cagcatggta acctctcggc accctgtctg aatggtgata
    16741 gaggttctga aggctctcca ccagcacatt gtaaaccttc tttctcagcc tgggggcttc
    16801 acgcgtcgca aacaactttg cctccacaag gtccaatgcc ttcgttattg tgtccgactc
    16861 aacatttccc ttgtaataaa ggactgctcc tctgatgtca aggtcgctca caagattttc
    16921 aaggtcaaag ctcatcgggc ctctgtgcaa gtatacaaag ctagaacgag taacaaaaat
    16981 atattattgt tgacaaaagc caacataccc tcttattatt ttggaaatga caaccttttt
    17041 ttgaatctga atgcaccggt tagtaacaaa acctgtttca ggcaccggaa acatttttat
    17101 gttttctggt tcctccgtag agctcatagc cctggaaacg gcattcaata tcaccgttga
    17161 gaaggtcgaa cttgcgaaaa ggtttaagcg ctaccttcct gagcgaatcc atgtcacccg
    17221 acaggatcca ggcagtgtaa ccctcataac ggtgcttcag cactgaaccg atcctgccat
    17281 agaactcatc catatcagat gttcccaggc gcctgccata tggcgggttg ataatcagca
    17341 ttccgttttc agccggagga ggtgattcaa agaaatcacg tccggatatc tcaacggtat
    17401 cctccaggcc tgctgccctg acgtttgctc tggcaatggc acaggcttca gcagatatgt
    17461 cactgccgaa tatcgcggct ggtgattttc tttcccgctt ttcggcatcg aacctgactg
    17521 acctgaagag tgcagcatca taatccttcc atctcatgaa tccgaaattt ttcctcattc
    17581 tcccgggagc aatattgcat gagatgagcc cggcttccgt cactatggta cctgatccgc
    17641 acatgccatc agtcagtggt gttccgggct tccagcccga aagggctatc agccccgcgg
    17701 caagcacctc gctcagagga gcctccgaca ttctccggcg gtaacctctc ctgtaaagag
    17761 gcaccaccgt tgagtcaatt gatatggtta ccctgttgtt gctgatgtga acgttcac
//
LOCUS       DAOPBK010000034        17057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3422_length_17057_cov_4.997647, whole genome shotgun sequence.
ACCESSION   DAOPBK010000034 DAOPBK010000000
VERSION     DAOPBK010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17057
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3422_length_17057_cov_4.997647"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLA17_03350"
     CDS             complement(<1..756)
                     /locus_tag="PLA17_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPK84479.1"
                     /translation="MKKRGINSVRTPVYRDSGFELPTAEATSGAFAAETGHEREPDLY
                     IYSRYRNPTVVAAEEAVMEVEGCGWALLAQSGMAAIDIALSIFQEAGRQSKWLFFSEI
                     YGGTLSYISSVLQKRRGIDVSLFRPVDGRYDYRQLESALSDFRPDILFFETISNPLLI
                     VAEGRRVIELGKKNGAKVLVDNTFATPMLWKPLSDGADLVIHSATKYLSGHGNITAGV
                     ICGNNDKLMQSAVEYRKYVGHMLSPDDAYRLQTQ"
     gene            complement(710..2233)
                     /locus_tag="PLA17_03355"
     CDS             complement(710..2233)
                     /locus_tag="PLA17_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPK84480.1"
                     /translation="MRKGFSFDAALCVSCKACSAACILENGFQPGTRSLFTWNEHALP
                     LVGVINLSMACNHCARPACASGCPALAYTIGPDGVVIHHADRCMGCGYCTWRCPYDAP
                     KINPALGYIEKCHLCTDRTAQNIEPACVAACPTGALKMTEREVFDGALPAWFPDTGIK
                     PSVFLKNSENYDRPLIIPADSESAKDEISAPLNTGADSEDDISALQDTRDDSEDDISA
                     LQDTGADSEDDISALPDTGDDSEDEISALPDTRIDSSVRLKKEWSLALFSLLVITASV
                     MMMVSALRGMATAGFIPPILLAGAMAVSLLHLGVPRRSWRAVLNILSSPLSREIAMVL
                     LLAFVSLAGWLKSDLFPPLITGLLALLTLISVDNVYFAADRSFSLKLHSGQAFFTGLY
                     AVTWFIEPTILFIVFSMLAALSVVMRYKSTEAGSLARTLYYIRAMALPVVFMLIYPDS
                     PLTDIAALVVFMAGLIADRLLFYCDIRPPNIKDTLTEHLEKEYEKKRDKQRQNAGIS"
     gene            complement(2230..4254)
                     /locus_tag="PLA17_03360"
     CDS             complement(2230..4254)
                     /locus_tag="PLA17_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPK84481.1"
                     /translation="MKEFITVCPRNCYSTCTFRVFTEDGRVMRILPYEGNLATPEGPC
                     IKGLSYLEREYSSERLIYPLGRQSDGSFVRIGTDEALNIIAERLCGAKAKHGPHSVLF
                     YSGSGSSGLSNDIAVSFWKLFGGATTTYGNLCWPAGLEAVRLTLGEVKHNLPWDIANA
                     SMIILWGKNSAETNVQEMIHIAKARQNGAKVVVIDPRRTLTADKADLLIRPRPGTDAA
                     LALAMARVIIDRGLTDREFISRHVSGFEQFAASLHITPAKAQEITGVPAQTIIDIAVE
                     AAVAGRLTIVAGYGLQRYTNGGQTIRSLLSIPVITGMIGKPGCGFNFANLQSYIFDDV
                     KEPLSYYPHSETDQPFRRAISMATFGQDFFALEEPAIEFAWIERGNPVIQLPGSSEVK
                     KALESVPFKVVVEQFMTDTAAMADIILPAKGILEQADVVGSYWNPYAQYKPKIVDPPG
                     EVLPESETYWRLARLMGLNADEAGIPAPEKYDDWLMKRISCTPGFAFEELMQKPLIPD
                     RNEHPAYANMKFATPSGKIELWSERAVKLWGVNPLPTYEPLDNFGEAHLPMRLMTPNI
                     ASRIHSQFGNLEVVRSVVEEPVWEISVADARSRGLKSGDMIRVFNSRGEVTGRVSVTH
                     RVKSGSLVFPNGIWLGEGGGVNRLIAPSETDMGHGAAFHNTRVDIEKMAP"
     gene            complement(4263..4682)
                     /locus_tag="PLA17_03365"
     CDS             complement(4263..4682)
                     /locus_tag="PLA17_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84482.1"
                     /translation="MKGKILMVLLASVLALTSCKKRNFTDKGPTDVRIHNQTGQVVSE
                     VTVTVTDDPAYSIRSHNYGTLAAGACSEYFRFDIAHTEADITVKIDDVIYSTPATQFK
                     YLTYIGPDRITYSITIVDAVNRILDIETIIEEPIDDL"
     gene            complement(4733..5491)
                     /locus_tag="PLA17_03370"
     CDS             complement(4733..5491)
                     /locus_tag="PLA17_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84483.1"
                     /translation="MKRIVLIQFIFLACMVTLAGQSPEERYAQAGELYGSGDYSGAAS
                     LYRKLWEEGYRSRDLLYNAGNAFFKSGDNASAILFYERAKLLAPADEDIDYNLQIARS
                     RITDRIESVPQLFFVRWFDFLALLTSTNTWAVAALVMFLAALVLALLFLTKARARGRL
                     LSFWLAIAALLFSFLMILLALRNNNLVNHNKKAVITCSIVTGRSAPGETGNELFVLHS
                     GTTVTIEQELGAFREVRLPDGNKGWIRSECMENI"
     gene            complement(5504..>6196)
                     /locus_tag="PLA17_03375"
     CDS             complement(5504..>6196)
                     /locus_tag="PLA17_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84484.1"
                     /translation="PRNTGTFDIPPVSYSVFDPGQEKYVTLRTGGFTLNVTGTPGQEE
                     GAAPVYIPGEDVKYIGQDIRFIRNGNGRLTTLSSPLVASLHYWLWFALALFIALLVLV
                     IRKQQIRRNADKAGMRNRRAAKNARKRLSRATALLKSGKKEEVNAEVARALWGYLGDK
                     LRIDQSDLTREKCYTALRERKADEEMITELDRILTATEYSQYAPVSEVESPAALCERA
                     ALLIGKLDNVLD"
     assembly_gap    6197..6296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6297..7126)
                     /locus_tag="PLA17_03380"
     CDS             complement(<6297..7126)
                     /locus_tag="PLA17_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPK84485.1"
                     /translation="MKRITTVLVFLLACLVAAAQEVTLTVDAPESAVAGERFRIIYTI
                     NSTDGQFTRPKFDPSFSVQGPQQSTNRNVQWINGDMKVVSSTTLIYYVTAGTPGTYTI
                     PAAQFETKRVTVSSQEKQIVIYDGDSSQQATGRQQGQTSAAGDAPASGSEVSMRILLS
                     DRDVYAGQPITGTLKLYTRINLSGIQELKLPDFKGFLKEDIETPPLRSLDNEVIDGVQ
                     YGTGVIQRFVLYPQIAGEIRIEPAEMTALVQERTTAARDPFFNDPFFDSFFSSVSTVP
                     R"
     gene            complement(7137..7886)
                     /locus_tag="PLA17_03385"
     CDS             complement(7137..7886)
                     /locus_tag="PLA17_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK84486.1"
                     /translation="MRGKETAYRLVVAVICAAAISPSLHGQEEREYIRKGNRLYRKSE
                     FAGSEGMYRLAEEEGKSSGDAVFNLGDALYRQNRFGEAAEEFSRAVQTGEADSLRQAD
                     GFYNLGNSLLKEQKFSESINAYINSLKLNPDNIQAKYNLAYAQDQLKKQEEQQQQQQD
                     QDQNQDQNQDKQNQQDQQNKDQDDNKDQEQKQQDDQQQQQQEQQQQQSSMSREDAKRL
                     LDALAANEKETQEKVQREKAAGARVRVIKNW"
     gene            complement(7883..8938)
                     /locus_tag="PLA17_03390"
     CDS             complement(7883..8938)
                     /locus_tag="PLA17_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPK84487.1"
                     /translation="METFRFAHPDMLYLLLLVPVLIIIWAIGNRKRRLARERFSDASM
                     LERLSPDYSPARMAVKFIIRLLAFIMAVLTVARPQFGSRLEEAKREGVEVVIALDVSN
                     SMLATDIIPSRLERAKQAITQLVDQLKEDRIGLILFAGDAYTQIPVTNDYLSAKMFLS
                     AAGPDAVSKQGTAIGAAINLGIRSFTPDSDKSRALIIITDGENHEDDAVEKAREAAEK
                     GIVVHTIGIGSPDGSPIPVMSGGRVDYLKDRQGNTVISKLDEKGLQEIASVTGGRYVR
                     ASNTSIGLTEIYNDIGRMKKSEINTMMYTEYNEQFLIPAALLFILLVTDLLLANRKSN
                     LLRRIGIIGKESKKERI"
     gene            complement(8946..9929)
                     /locus_tag="PLA17_03395"
     CDS             complement(8946..9929)
                     /locus_tag="PLA17_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPK84488.1"
                     /translation="MVFENGWFLWFLLIIPLLVAHYIWRQGKANAPVKLSTAGFFAEG
                     RKTFRHYLRHLLFALRVTAIALIIVVIARPQSVDRWQSSTTEGIDIILALDVSGSMLA
                     RDFSPDRLEASKNVATEFISGRPYDRMGLTVFSGESFTQCPLTTDHAVLINLLREVKS
                     GIIEDGTAIGVGLATAINRIKDSDAISKVIILLTDGVNNRGSIDPMTAAEIAKTFGIR
                     VYTIGVGSMGYADYPVQTPFGMRYQKMQVEIDEALLQKIAEMTGGHYFRAVDNTSLQK
                     VYGEIDKLEKSKIETREHSRRDEVFMSWAFAALVLFSLELLLRYLFMKNMS"
     gene            complement(9931..10959)
                     /locus_tag="PLA17_03400"
     CDS             complement(9931..10959)
                     /locus_tag="PLA17_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84489.1"
                     /translation="MRKAFISILVLLASGVLLSARQIRVTSKPDTSVILIGDQIGFTV
                     TAELPSGLSAYMSGAADTLAGKIVILRRDPRDTALSADGSVTITDRYLITAFDSGSYT
                     IPPFYAEVASGDSLLRFFSDYSFLDVLRPDVTPPDTTDVIFDIVGPRSAPVTFREILP
                     WIIITLIVVVILWLLARYLPRNPLERFMRPPAPPEPAHVIALRDLKALRSEEIWQKGE
                     IKEYYSRLSDILRRYISNRYGISSPELTTDETVRMLQKASVTTAGQMAVVKEVLSLSD
                     MVKFAKYLPDSALHESCMDSAVSFVEETRLPDAVPADSTDKSDKTENGDGAGALPGRE
                     NEKGGLHA"
     gene            complement(10964..11827)
                     /locus_tag="PLA17_03405"
     CDS             complement(10964..11827)
                     /locus_tag="PLA17_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPK84490.1"
                     /translation="METTELLKKVRKIEIRSRGLTRQIFAGKYHSAFRGRGMTFSEVR
                     EYQVGDDIRNIDWNVTARFGHPYVKVFEEERELTVMLLVDVSRSGDFGTVASTKREMM
                     TEIAAVLAYSVIVNNDKVGLMLFSDRVEKYIPPKKGRSHMLRIIREMLGYKPSGSATS
                     LSEPLRYLTNIIRKRCTAFILSDFIAPPFTDALRIASGKHDVVAIRVSDRREKEMPDV
                     GFIKILNPETNREEWIDTSFSAVRRDYAAQWKKHDEAVGSAFVSCGVDSISVSTGEDY
                     IKPLISLFRHR"
     gene            complement(11842..12246)
                     /locus_tag="PLA17_03410"
     CDS             complement(11842..12246)
                     /locus_tag="PLA17_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84491.1"
                     /translation="MKRLQLSLILLIFMIPVKAQEFYGMTEKNIRALMERDYQGLTPD
                     NMVRNNLFRYLRYHSADDDETWIIFLDDRNRCKGVRITYSNTLYDTKISELNRKYGYG
                     EGGKWSYRLERNRIAVTVHRDEWFFTVTHVRM"
     gene            complement(12251..13246)
                     /locus_tag="PLA17_03415"
     CDS             complement(12251..13246)
                     /locus_tag="PLA17_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPK84492.1"
                     /translation="MEHTTDIRLLNEKIEKESAFVDLVRQEINKVIVGQRHLTDSLLI
                     GLLSNGHILLEGVPGLAKTLAIKTLASIIDTKFSRIQFTPDLLPADLIGTLIYSQRKE
                     EFVVRKGPIFANFILADEINRSPAKVQSALLEAMQERQVTIGDTTYRLDEPFMVLATQ
                     NPIEQEGTYPLPEAQVDRFMLKVVITYPEKEEERLIIRENLAREFPVAKTILKPEDII
                     RARSVVRDVYLDEKIENYILDIVFATRQPDKYGLSKFTPLIAYGASPRAGINLALAAK
                     AYAFIRRRGFVIPEDVRSLCADVLRHRIGLTYEAEAENITQDQIIAEILNTVEVP"
     gene            complement(13341..14498)
                     /locus_tag="PLA17_03420"
     CDS             complement(13341..14498)
                     /locus_tag="PLA17_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPK84493.1"
                     /translation="MIKKTILLAALMMLVIAPDIEARTADSTDVYMITCAPGNESYSI
                     YGHTALRIAIRGSDFDMVYNWGIFDFSTPNFVYRFARGRLDYMLGAYSYSRFLQEYIS
                     EGRSVWSQKLNLTTTEKERLFLLINENLRPENVKYRYDFFYDNCATRVRDIIAASADD
                     TVIFPVKEKSFSFRQLIDPYQKVLPWLDLGADMLLGLPADRKASVADEMFLPILLRDN
                     FAGTVIVHDGIEESLAEPLETVVDIPATQTASKAWVPQALFWLIFAFVVLVTFVFKRP
                     GLEKVIDYILYFIFSVIALLLIFTNIFSEHDALHLNLLFLGINVLIALLFIMMIAGKK
                     SVKLSRLTLTLSLLWFPVALIAGQGINPALIPLVLTIMVRLFRHCSFGKEL"
     gene            complement(14636..16138)
                     /gene="glpK"
                     /locus_tag="PLA17_03425"
     CDS             complement(14636..16138)
                     /gene="glpK"
                     /locus_tag="PLA17_03425"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPK84494.1"
                     /translation="MKKRYILSLDQGTTSSRAILFGSDGQPVASSQKEFTQIFPQPGR
                     VEHDPAEIWSSQAGVVAEVVAGSGADYSDIVAIGITNQRETTVVWNRRSGRPVYNAIV
                     WQDRRTADMCDTLRAEGHGAMIAEKTGLVIDAYFSATKVKWILDHVDGARAAAERGDL
                     AFGTIDSWLVWNLTGGRLHVTDVSNASRTMLFNIRSLQWDEELLSLFEIPSSMLPEVR
                     SSSEIYGHSTGLFASGIPVAGIAGDQQAAMLGQMCVDEGMVKNTYGTGCFMMMNTGSE
                     PMRSGNRLLTTVAWKIGDNTTYALEGSIFIGGAVVQWLRDGLGIIRQSSDVETLASRV
                     SSAEGVCFVPAFAGLGAPYWNQHARGTITGLTRGTTAAHIARAALESIAFQTLDVLLA
                     MEKDSDLKIKELRVDGGATVNDSLMQFQADILQTDVLRSAVTETTALGAAFLAGLATG
                     FWGGMDDLRGLWQAGHHFVPAMKPEERNRLIREWNRAVKSAAAWADDAVR"
     gene            16296..16934
                     /locus_tag="PLA17_03430"
     CDS             16296..16934
                     /locus_tag="PLA17_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPK84495.1"
                     /translation="MVNRYIITIGRQFGSGGREIGQKLSARLGIAFYDKELIRIASQQ
                     SGVKEEFFERVDEQKHFSLFPGILGLRTSLTDDFFPNYYLSNESLFRIQSDVIRRLAG
                     EGSCIFVGRCADYVLKEERNCLTLFISADMADRIKRIAVRHKLSESKAKDLIERTDKG
                     RSSYYNYFSGKTWGAAESYHLCINSSLAGIDETVRLISHVAGIRFGLTEKRE"
BASE COUNT         3956 a         4518 c         4163 g         4320 t
ORIGIN      
        1 ctgtgtttgc agacggtagg catcgtcagg cgagagcatg tgccccacat atttgcggta
       61 ctctacggca ctctgcatca gtttgtcatt gttgccgcat atcacaccgg cagtaatgtt
      121 gccgtgacca gagagatact tggtggcgct gtggatcaca aggtcagctc cgtcactgag
      181 aggtttccac agcatcgggg tggcaaaggt gttgtctacc aaaaccttgg ctccattttt
      241 tttgccaagc tcaatgactc ttcgtccctc tgcaacgata agcaacgggt tagagattgt
      301 ctcgaaaaag agaatgtcag gcctgaaatc agacagtgcg ctttcaagct gccggtagtc
      361 atacctgccg tcaacgggtc tgaagagact cacatcaatg ccgcggcgct tttgcagcac
      421 gctgctgatg tatgacagtg tgccgccgta gatctccgaa aagaagagcc atttgctctg
      481 tctgcctgcc tcctggaaga tcgaaagggc tatgtcaatg gcggccatgc ctgactgtgc
      541 cagcagcgcc cagccacacc cctcaacttc catgactgcc tcctcagcag caacaaccgt
      601 cgggttgcgg tatcgtgaat atatatacag atcaggctcc cgctcatgac ccgtctctgc
      661 tgcaaaagcg cccgatgtgg cttctgctgt aggaagttcg aagccggaat cacgatatac
      721 cggcgttctg acgctgttta tccctctttt tttcatattc tttctccaga tgttctgtta
      781 atgtatcctt tatatttggc ggcctgatgt cacagtagaa aaggagcctg tcagcaatca
      841 agcctgccat gaaaaccacg agggctgcaa tgtctgttaa cgggctgtca gggtagatca
      901 gcataaaaac aacaggcaac gccattgccc ttatgtaata gagtgtgcgc gcaaggctgc
      961 cggcctccgt ggatttgtat cgcattacca ccgaaagggc agccagcatc gaaaaaacaa
     1021 taaagagtat ggtgggctca atgaaccatg tgacagcata caagcctgta aaaaaagcct
     1081 ggccgctgtg aagtttcagg ctgaacgacc tgtcagcggc gaaatagaca ttatctacgg
     1141 agattagtgt cagcagtgcc agcagccccg ttatcagcgg cggaaaaagg tctgatttca
     1201 gccaccccgc tagtgacacg aaggcaagca gaagaaccat tgctatctca cggctcagtg
     1261 gagatgagag gatgttgaga actgccctcc acgacctccg gggtactccg aggtgcaaaa
     1321 gggaaacagc cattgctccg gccaggagga ttgggggaat gaatccggcg gttgccattc
     1381 cccggagtgc ggagaccatc atcatgaccg aggctgttat tacgagcagc gaaaagagcg
     1441 caaggctcca ctcctttttc agccgtacag aagaatcaat tctggtgtcc ggcagcgcag
     1501 agatctcatc ttctgaatca tctccggtat ccggcagtgc agagatatca tcttctgaat
     1561 cagccccggt gtcctgcaat gcagagatat catcttctga atcatctctg gtgtcctgca
     1621 gtgcagagat atcatcttct gaatcagccc cggtgtttag cggcgcagat atctcatctt
     1681 ttgccgattc actgtcggca ggaattatca gcggcctgtc atagttttca ctattcttca
     1741 ggaaaacaga cggttttatc cccgtatcag gaaaccatgc cgggagagcg ccgtcaaata
     1801 cctccctttc tgtcattttc agcgctcctg tcgggcaggc ggcaacgcag gccggctcaa
     1861 tattctgagc ggttctgtca gtgcataagt ggcatttttc aatatatccc agggctggat
     1921 tgatcttcgg ggcgtcatag ggacacctcc atgtgcagta tccacacccc atgcaccggt
     1981 ctgcatgatg gataacaacg ccgtcaggcc caatggtgta tgccagcgcc gggcagcctg
     2041 aagcgcaggc gggccgggca cagtggttgc aggccatgga gaggttgatg acgcccacca
     2101 gcggaagtgc atgttcattc cacgtgaaga gggagcgtgt gccgggttgg aacccgttct
     2161 caagaatgca ggcagcgcta caagccttgc agcttacaca gagtgcggcg tcaaatgaga
     2221 atcccttcct catggcgcca tcttttctat gtcaaccctc gtgttgtgaa aggcagcacc
     2281 atgccccata tcggtctctg acggcgctat gagcctgttc actcccccgc cctcgccaag
     2341 ccatataccg ttgggaaaga ccaggctgcc gcttttgacg cggtgtgtga cgctgactct
     2401 tcccgtaacc tctccgcgtg agttgaacac cctgatcatg tcgccacttt tcagacccct
     2461 gctccttgca tcagcaaccg atatttccca taccggctct tcaaccacac tcctgacaac
     2521 ttccagatta ccgaactgtg aatggatacg tgatgcaata ttcggtgtca tgagcctcat
     2581 gggaagatgc gcctccccaa agttgtccag tggttcatag gtcggcagcg gattcacacc
     2641 ccacagcttc actgcccttt cagaccagag ctctatcttt cccgaaggag tggcaaattt
     2701 catgtttgcg taggccgggt gctcgttccg gtcaggtatc agcggcttct gcatcaactc
     2761 ctcaaatgcg aaacccggtg tgcagcttat cctcttcatg agccagtcgt catacttctc
     2821 gggggctgga atgccggcct catccgcgtt cagacccatc agccgggcca accgccagta
     2881 tgtttcgctc tcgggaagca cctcgccggg cggatcaaca atcttaggtt tgtactgggc
     2941 atacggattc cagtaggagc ccaccacgtc agcctgttca agtattccct ttgcaggaag
     3001 gatgatgtca gccatcgctg ccgtgtcagt cataaactgt tctactacaa ccttgaaagg
     3061 aaccgattcc agcgcttttt tcacctcgct gctccccgga agctgaatga ccggattccc
     3121 tctctctatc caggcaaact ctattgccgg ctcctccagg gcaaagaagt cctgcccgaa
     3181 ggttgccatg gagatggcac gcctgaatgg ttggtcagtt tcagaatggg gataataact
     3241 caggggctcc tttacatcat caaatatgta gctctgcagg ttggcaaaat tgaacccgca
     3301 tcccggcttg ccaatcatgc cagtaattac cggaatggaa agaagtgacc ttattgtctg
     3361 ccctccgttg gtatagcgct gcaacccgta gccggccaca atggtcagac ggccggcaac
     3421 ggctgcctcc actgcaatat ctatgattgt ctgagctggt actcctgtaa tttcctgtgc
     3481 tttggcaggt gttatatgaa gtgatgctgc aaactgctca aagcccgaaa catgcctgct
     3541 gatgaactcc ctgtcggtca gtccacggtc aattatcacc cgggccatag ccagcgccag
     3601 ggctgcgtcg gtgccgggcc tgggtctgat gagcaggtca gccttgtcgg cagtgagggt
     3661 acgacgcggg tctatgacaa ccacttttgc tccgttctgt cgcgccttcg cgatgtgaat
     3721 catctcctgc acgttggtct cagcggagtt ctttccccag aggatgatca tggacgcgtt
     3781 tgcaatgtcc cagggcaggt tgtgcttaac ctcacccagt gtaagcctga cagcttcgag
     3841 tccggccggc cagcacaggt tgccgtaggt tgtggtggct ccgccgaaca gtttccagaa
     3901 actgacggca atatcattgc tcagccctga ggaaccactg ccactgtaaa acaacactga
     3961 atgcggacca tgctttgcct tcgccccgca gagtctctct gcaatgatat tcaaggcttc
     4021 atccgtgccg atgcgcacga aggagccgtc agactgcctc ccgagaggat aaatcaaccg
     4081 ttctgaagag tattcccttt caaggtagga aagtcctttg atacacggcc cttcgggtgt
     4141 agccaggttg ccctcgtaag ggagaatgcg catcaccctg ccatcttccg tgaataccct
     4201 gaaggtgcat gtactgtaac agttgcgcgg acataccgtg atgaactcct tcattcccgg
     4261 ggctaaagat cgtcaatagg ctcctcgatg attgtttcga tatccagtat ccggttcacc
     4321 gcatcaacta ttgtaatact gtaggtgatc ctgtcaggac cgatgtaagt caggtattta
     4381 aactgcgttg ccggcgtgga ataaataaca tcgtcaattt ttacagtgat gtcagcctcg
     4441 gtatgggcaa tatcgaatct gaagtattca gagcaggctc ctgcagccag agtgccgtaa
     4501 ttatgtgacc ttattgagta tgccgggtca tcagtgacgg tgaccgttac ttcgctgaca
     4561 acctgacccg tctggttatg gatacggacg tcggtcgggc ccttgtcagt gaagttgcgt
     4621 ttcttgcatg atgtcagcgc cagcacggat gccagcaaaa ccatcaggat ttttcctttc
     4681 atgtccggac aaattgattc cgggagaaag ttaagaaaat agttttaacc aattatatat
     4741 tttccatgca ctctgacctg atccatccct tgtttccatc gggaagtctc acctcgcgga
     4801 atgcccccaa ctcctgttca atggtgacgg ttgtgcctga atggagtaca aagagctcat
     4861 tgccggtctc acccggggcg cttcggcccg taacgatgct gcaggtaatc actgcttttt
     4921 tgttatgatt gacgaggtta ttgttccgaa gagccagtaa aatcatcagg aaagagaaca
     4981 gcagcgctgc gatagcgagc cagaacgaga gaagccgtcc ccttgccctg gcctttgtga
     5041 ggaacagaag tgcaagcacc agggctgcaa ggaacatcac aagggcagcc actgcccagg
     5101 tatttgtcga tgtaagcagc gcaagaaaat caaaccacct gacaaaaaac agctgtggaa
     5161 ccgattcaat cctgtctgta atacggctcc tggctatctg caggttatag tcaatatcct
     5221 catctgcagg cgccagaagc tttgcccttt cataaaagag gatggcggag gcattgtcgc
     5281 ctgacttgaa gaaagcgttt cccgcattgt agaggagatc cctgctcctg tacccctcct
     5341 cccaaagctt cctgtagagg gatgcagcac ctgaatagtc acctgaacca tagagctcgc
     5401 ctgcctgggc ataccgttct tcgggtgatt gcccggcaag agtaaccatg caggcaagga
     5461 atataaattg tataagcact attcttttca tcttcttcct cctctagtcc agtacattgt
     5521 ctagtttccc gataagcagg gcagctctct cacatagtgc cgcagggctt tctacttcag
     5581 ataccggagc atactgtgag tattcggtag ccgtgagtat ccggtcgagc tccgtgatca
     5641 tctcctcatc tgccttcctc tctctcagtg ccgtgtaaca cttctcccgg gtgagatcag
     5701 actggtctat gcgtagtttg tcaccgagat aaccccagag tgccctggca acttcggcat
     5761 tcacttcctc cttctttcct gatttgagaa gtgcagtggc cctggagagt cttttgcggg
     5821 cattctttgc cgcccggcgg ttccgcattc ctgccttgtc agcattgcgc ctgatctgct
     5881 gcttacgtat aacaagcacc aggagtgcaa taaagagggc aagcgcaaac catagccagt
     5941 aatgcagtga tgccaccagc ggtgaggata gcgtggtcag cctgccattg ccattccgga
     6001 tgaacctgat atcctggccg atatacttca catcctcccc gggaatatag accggagcag
     6061 cgccttcctc ctgcccgggt gtgccggtga cgttgagagt gaatccgccg gtgcgcagtg
     6121 tcacatactt ttcctgcccg ggatcgaaaa ccgaatatga tacgggaggt atatcgaagg
     6181 tgccggtatt tctcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg
     6301 gtacggttga tactgatgaa aagaaactgt caaagaacgg atcgttaaag aagggatctc
     6361 ttgcagctgt agtccgttcc tggaccaggg cggtcatctc tgccggctca atgcgtatct
     6421 cacccgcgat ctggggatag agcacgaaac gctggatgac gccggtacca tactgaacac
     6481 cgtcaatgac ctcattatca agactgcgca aaggcggggt ctcaatatct tccttcagga
     6541 aacccttgaa gtcaggaagc ttaagttcct gtattccaga aagattaatg cgggtgtaga
     6601 gcttcagcgt tccggttata ggctgacctg cataaacatc acggtcactg agcagtatcc
     6661 tcatggagac ctctgaccca gaggcaggag catcgccggc tgcacttgtc tgtccctgct
     6721 gccggcctgt tgcctgctgc gacgaatctc cgtcatatat gactatctgt ttttcctgcg
     6781 agctgactgt gactcttttg gtttcgaact gagccgccgg aatggtgtaa gtgcctggtg
     6841 taccagcagt aacatagtaa ataagggtgg tggaggaaac cactttcatg tctccgttga
     6901 tccactgcac attacggttg gttgactgct gaggaccctg cactgagaat gaaggatcga
     6961 acttcggccg ggtgaattga ccgtctgtag agttgatggt atagatgatt ctgaaccttt
     7021 ccccggcaac ggcgctctcc ggtgcatcaa cggtaagcgt tacctcctgg gcagctgcca
     7081 caaggcaggc cagcagaaaa accaataccg tcgttattct tttcatcact gcaaaattac
     7141 cagtttttaa tgacccttac ccttgccccg gcagcctttt cacgctgcac cttctcctga
     7201 gtctcctttt cattggcagc aagagcgtca agaagacgct ttgcatcctc acgactcatg
     7261 gatgactgct gctgttgttg ttcttgctgt tgctgctgct gatcatcctg ctgcttctgc
     7321 tcctgatcct tgttatcgtc ctgatccttg ttctgctgat cctgctgatt ctgtttatcc
     7381 tggttctgat cctggttttg gtcctgatcc tgctgctgct gttgttgttc ttcctgtttc
     7441 ttcagctggt cctgggcata agccaggttg tactttgcct ggatattgtc aggattgagt
     7501 ttcagcgagt tgatgtaggc attgatactc tcgctgaatt tctgctcctt cagcagtgaa
     7561 ttgcccaggt tatagaatcc gtcagcctgc ctgagtgaat ctgcttcacc ggtctgtact
     7621 gcacggctga attcctccgc agcttcgcca aacctgttct gcctgtaaag ggcgtcaccg
     7681 aggttgaaga ccgcatcgcc tgaagatttt ccctcctcct ctgcaagacg gtacattcct
     7741 tcactgccgg caaactcact tttcctgtaa agcctgtttc ccttcctgat atattccctc
     7801 tcctcctgtc cgtggagtga aggagagatt gctgccgcgc agatgacggc aacaaccaac
     7861 ctgtaagctg tttccttccc tctcatatcc tttccttttt ggattctttt ccgataatcc
     7921 ctatacgcct gagaagattg ctcttcctgt tggccagcag caggtcagtc acgagcagta
     7981 tgaagagaag cgccgcaggt atgaggaact gctcattgta ctcggtgtac atcatggtgt
     8041 tgatctcact ctttttcatc cggccaatgt cattgtaaat ctcggtcagt cctatgctgg
     8101 tattgctggc cctgacatat ctaccgcccg ttaccgacgc aatctcctgc aaacctttct
     8161 catcaagctt cgaaatgacc gtatttccct gtctgtcctt aagataatcc actcttccgc
     8221 cactcatcac cggtatggga gatccgtcag gcgagcctat acctattgta tgcaccacga
     8281 tacccttctc agccgcttcg cgtgcctttt caaccgcatc atcctcatgg ttctcaccat
     8341 cagtgatgat tatcagcgcc ctgctcttgt cactgtcagg agtgaatgac ctgattccca
     8401 gattaatggc agcgccgata gcggttccct gctttgaaac ggcatcaggt cctgctgctg
     8461 acagaaacat tttggcggag agatagtcat tggtaaccgg tatctgtgta tatgcatctc
     8521 cggcaaagag aatgaggccg atgcggtctt ccttcagctg atcaacgagc tgggttatag
     8581 cctgtttggc cctttcgagc cttgagggaa ttatatcggt agccagcatc gagttgctca
     8641 cgtcaagggc tatcaccact tccactccct cacgtttagc ctcttccagc cgcgagccga
     8701 actgtggtct tgcaacggtg agcacggcca taatgaaggc cagaagcctg atgatgaatt
     8761 taaccgccat tctggcaggc gaataatcag gcgagagccg ttccagcatt gacgcgtcgc
     8821 tgaagcgttc cctcgccagt ctccttttcc tgttgccgat agcccatatt ataatcagca
     8881 ccggcaccag gaggagcagg taaagcatgt caggatgtgc aaatctgaat gtttccataa
     8941 tcgcgctatg acatgttttt cataaagaga tacctcagca gaagctcaag ggagaagagg
     9001 acaagagctg cgaacgccca tgacatgaac acttcatccc gcctgctgtg ctccctggtc
     9061 tctatctttg atttctcaag cttgtcaatc tcaccgtaca ccttctggag gctggtattg
     9121 tccactgccc tgaaatagtg accgccggtc atctctgcaa tcttttgaag aagagcttca
     9181 tctatctcca cctgcatctt ctggtatctc atgccaaagg gtgtctgaac cggatagtct
     9241 gcatacccca tgcttcccac accgattgta tataccctga tgccaaatgt cttcgctatc
     9301 tccgctgccg tcatggggtc aattgatccc cggttgttta cgccgtcagt cagcaggatt
     9361 atgactttac tgatggcatc tgaatccttt atcctgttga ttgcagtggc cagtcccacg
     9421 ccaatggctg tgccatcctc aataataccg ctcttcactt ccctgagcag gttaataagt
     9481 acggcatgat ctgttgtcag ggggcattgc gtaaaactct caccactgaa cactgtcaac
     9541 cccatgcggt catacggtct tccgctgatg aattccgttg cgacgttctt ggatgcttcc
     9601 agcctgtcgg gactgaaatc acgggcaagc attgatcccg acacgtcaag ggcaaggata
     9661 atatcaatcc cctcagtggt ggagctctgc cacctgtcaa cactctgcgg cctggcaatt
     9721 accactatta tcagcgctat ggctgtcacc ctgagtgcaa agagcaggtg tctcaggtag
     9781 tggcggaatg ttttgcgccc ttcggcgaag aatcctgccg tggagagttt taccggggca
     9841 tttgcctttc cctggcgcca gatgtagtgt gccaccagga gcggtatgat gagcagaaac
     9901 cagaggaacc agccgttctc gaagacaatg ttatgcatgg agccctcctt tctcattttc
     9961 ccttccgggg agtgcaccgg caccgtctcc gttttccgtc ttatcagatt tatccgtgga
    10021 atcagccgga acggcatccg gaagcctggt ctcttcgaca aaactcacgg cactatccat
    10081 gcatgattca tgcaaagcac tgtcgggcag atatttggca aacttaacca tgtctgagag
    10141 tgacaatacc tccttaacaa cagccatctg tcccgcagtt gtcaccgatg ccttctgcag
    10201 catcctcaca gtctcgtctg tcgtcagttc gggagagctg ataccgtaac ggttggaaat
    10261 atatctcctg agtatgtccg agagccttga gtaatactcc ttgatctccc ccttctgcca
    10321 tatctcctca ctcctgaggg ccttcaggtc acgcagtgca atgacatgtg ccggttcggg
    10381 cggggctggc ggcctcataa accgctcaag cggattcctt ggcaggtagc gtgcaagcag
    10441 ccacaggatc accacgacaa tcagggtgat tattatccaa ggcaggattt cccggaaggt
    10501 tacgggggcg cttcgcgggc caactatgtc aaaaataaca tcagtagtgt caggcggggt
    10561 gacatcagga cgaagcacat ccagaaatga gtaatcagag aagaatctga gcaggctgtc
    10621 gccggaagcc acctccgcat agaatggcgg aatggtatat gaaccggagt caaatgccgt
    10681 aatgagatac ctgtcagtga ttgttaccga cccgtctgct gagagcgctg tgtcacgagg
    10741 gtccctgcgc aatattacaa tctttccggc cagggtgtcg gcagcaccgc tcatataggc
    10801 agagagacct gaaggcagct ccgcggtaac agtaaagcca atctgatcgc caataagtat
    10861 gactgaggtg tcaggcttag aggtaaccct tatctgccgg gctgacagga gaactcctga
    10921 tgcaagtaaa accaatattg aaatgaaagc ctttctcata ttactacctg tgcctgaaca
    10981 gtgagataag tggtttgata taatcttctc cggtgctgac ggatatcgaa tcaactccgc
    11041 aactgacaaa ggctgagcca actgcctcat catgcttttt ccactgcgcg gcataatccc
    11101 tcctgacagc cgaaaaggag gtgtctatcc attcttcacg gttggtctcc ggattcagaa
    11161 tcttaatgaa gcccacgtca ggcatctcct tctcacgcct gtcactgacg cggatggcaa
    11221 ccacgtcatg tttgcccgat gcgatacgca gggcatcggt aaatggcggt gcgatgaaat
    11281 cggacaggat aaaggcggta cacctcttcc ttattatgtt ggtaaggtat ctcagcggct
    11341 cagagagtga tgtggctgat cctgaaggct tataaccgag catctcacga atgatcctga
    11401 gcatatggct tctgcccttc ttcgggggaa tatatttctc cacacggtca gaaaagagca
    11461 tcaggcctac cttgtcattg ttgacaatta ccgaatatgc aaggacggca gctatctcgg
    11521 tcatcatctc cctcttggtg gaagccaccg taccaaaatc tcccgaccgg ctcacatcaa
    11581 ccagcagcat gactgtcagc tctctctcct cctcgaagac cttgacatat ggatgaccaa
    11641 accgggcggt gacgttccag tctatgttgc ggatgtcatc accaacctgg tattcgcgca
    11701 cctcgctgaa ggtcattccc ctgccacgga aagcactgtg atatttacct gcaaagatct
    11761 ggcgggtcag cccgcgcgac ctgatctcaa tcttccttac cttctttaac agttctgttg
    11821 tttccacttc acttacaggt gttacattcg cacatgtgtg actgtaaaaa accattcatc
    11881 acggtgaacc gtgactgcaa tccggttccg ttccagcctg taactccact ttccgccttc
    11941 gccatatccg tacttcctgt tcagttcact gatcttcgta tcgtaaagag tgttgctgta
    12001 tgttattctg acgcctttgc acctgtttct atcgtcaagg aatataatcc atgtctcatc
    12061 gtcgtcagcc gaatggtatc tgagatacct gaagagattg ttcctgacca tgttgtcagg
    12121 cgtcagaccc tgataatccc tctccatgag agcacggatg ttcttttcag tcatgccgta
    12181 gaattcctga gcctttacag ggatcataaa tatcagcagg atcagtgaca gttgtaatct
    12241 cttcatcctt ctacgggact tcgactgtat taagtatctc tgcaatgatc tgatcctgag
    12301 tgatattttc agcctcagcc tcgtatgtca gtcctatgcg gtggcggagg acatcggcac
    12361 agagggagcg gacatcttcg ggaatcacga atccccttcg ccttataaag gcatatgctt
    12421 tagcagccag cgcgaggttt atccctgccc tgggcgaggc tccgtaggct atcagcgggg
    12481 tgaactttga gagaccgtat ttgtcgggtt gacgggtggc aaatacaatg tcaaggatat
    12541 agttttcgat cttctcgtca aggtaaacat ccctgaccac gcttctggcg cggattatat
    12601 cctccggctt cagtattgtc tttgcaacgg ggaactctct ggccaggttc tccctaatga
    12661 taaggcgttc ctcctccttc tcggggtagg tgatcaccac cttaagcatg aacctgtcaa
    12721 cctgcgcttc cggcagcgga taggtaccct cctgttcgat agggttctgt gttgccagca
    12781 ccataaacgg ctcatcaagc ctgtatgtgg tatcacctat ggtcacctga cgctcctgca
    12841 ttgcctccag gagggcgctc tgaaccttgg ccggtgagcg gttgatctca tcggcaagaa
    12901 tgaaattggc aaagatgggc cccttgcgta caacaaactc ctcttttcgc tgactgtaga
    12961 tcaatgttcc gataaggtca gcaggcaaaa ggtcaggagt gaactgaatc ctgctgaatt
    13021 ttgtgtcaat gatcgaagcc agggtcttga tggcaagtgt tttggccaga ccaggcacac
    13081 cctcaagaag gatatgcccg ttggaaagaa gcccgataag cagtgaatca gtcagatgac
    13141 gctgacccac tattaccttg ttaatctcct gcctgacaag atcaacaaag gcgctttcct
    13201 tttcaatctt ctcattcaga agcctgatgt cagttgtgtg ttccatatac tgttatattg
    13261 taattccttc ctgagcttaa aagcttcgca aagatcattt ctgatattaa aaatccttgt
    13321 taaatgttgt taaagatgtg ttaaagctcc ttcccgaagc tgcagtgcct gaagagcctg
    13381 accatgattg tcagcaccag cggtatcagc gccgggttta tcccctgccc ggcaatgagg
    13441 gcaacaggaa accacaggag tgacagggta agagtcaacc tgctcagctt caccgatttt
    13501 ttcccggcaa tcatcatgat gaaaaggagt gcaatcagca cgtttatccc aaggaaaagc
    13561 aggttcagat gaagtgcatc atgttccgag aagatatttg tgaaaatcag caagagtgca
    13621 attacggaaa atatgaaata gagtatgtaa tcaatcacct tctccagccc cggccttttg
    13681 aaaacaaatg taacgagcac aacaaatgcg aatatgagcc agaagagagc ctgcggcacc
    13741 catgccttac tggcggtctg tgtggcaggg atgtctacca cagtctccag aggctctgcc
    13801 agcgactcct ctattccgtc atgaactatg acggttccgg cgaagttgtc tctcagcaat
    13861 atgggcagga acatctcatc agccactgag gcttttctgt cagccggcag tccgagcagc
    13921 atgtcggcgc caagatccag ccacggaagc accttctggt aggggtctat cagctgacga
    13981 aagctgaagc tcttctcctt caccggaaaa atgacggtgt catcagcaga ggcagcaatg
    14041 atgtcgcgta cccgggttgc gcagttgtca taaaagaagt cgtacctgta cttcacgttt
    14101 tctggccgga ggttctcatt gataaggaga aacagcctct ccttctccgt tgtggtcagg
    14161 ttaagcttct gagaccatac agacctcccc tcggatatat attcctggag gaacctgctg
    14221 taactgtatg ctcccagcat ataatcgagc ctccccctgg caaacctgta gacgaagttg
    14281 ggtgtggaaa agtcaaatat gccccagtta tagaccatgt caaaatctga accgcgtatg
    14341 gcaatcctga gtgcggtatg gccgtatata gagtagcttt cattacccgg tgcgcaggta
    14401 atcatatata catctgtact gtcagcagtc cgggcttcaa tatccggtgc aatgaccagc
    14461 atcatcaggg cagccaggag gattgttttt tttatcatga tacaattctg attttaaagt
    14521 gtgattgaac ccgcatgact gagaatttta tgaacaggtg tttgtctgtt tggttcatgt
    14581 gggtttcatt atttttctcg acaaaggtct ttctgattat ttgttgcaga tcttatcatc
    14641 ttacggcatc atctgcccat gcagcggcac tcttcaccgc acggttccat tcgcgtatga
    14701 gcctattcct ttcctccggt ttcatagccg ggacaaaatg atgtccggcc tgccagagac
    14761 cccgcaggtc atccatgccg ccccagaacc ctgttgccag accggcaaga aaagctgcac
    14821 ccaatgctgt cgtttccgtt actgctgaac gaagtacgtc tgtctgaaga atgtcagcct
    14881 ggaattgcat caggctgtca ttgacagtag ctccgccatc gactcttaac tcttttattt
    14941 tcagatcaga atccttttcc atcgcaagta acacatcaag tgtctgaaat gctatgcttt
    15001 caagggcggc acgggcgatg tgggctgcgg ttgtaccccg tgtcaggccg gtaatggtac
    15061 ccctggcatg ctggttccag tatggcgctc ccaggccggc gaatgccggg acaaagcaga
    15121 cgccttccgc cgagcttacc cgtgatgcaa gggtctccac gtcagatgac tgccgtatga
    15181 tgccgagtcc gtcgcgcagc cattggacca ctgccccgcc tataaagata ctgccctcaa
    15241 gggcataggt tgtgttatct cctatcttcc atgccaccgt agtaagcagc ctgttgccgg
    15301 atctcatcgg ttcacttccg gtattcatca tcataaaaca accggtacca taggtgttct
    15361 ttaccattcc ttcatctacg cacatctgtc ccagcatggc agcctgctgg tctcccgcta
    15421 tccctgcgac gggtatcccg gaggcaaaga ggccggtaga atgaccgtaa atctctgatg
    15481 atgaacgcac ttcaggcagc attgatgaag gtatttcaaa gagggaaaga agttcttcat
    15541 cccattgcag gctgcggata ttgaacagca tggtgcgtga tgcatttgaa acatccgtga
    15601 catgcagcct gccgcctgtc aggttccata ccagccatga gtcaatagtt ccgaatgcaa
    15661 ggtcacccct ctcagctgct gccctggcac catcaacatg gtcaagaatc cattttacct
    15721 tggtggccga gaagtaggcg tcaatcacca ggcctgtctt ttcagctatc attgcgccat
    15781 ggccttcagc cctgagggta tcacacatat ccgctgtgcg acggtcctgc cagactatgg
    15841 cattgtacac aggcctgccg cttctcctgt tccataccac ggttgtctcg cgctggtttg
    15901 tgatgccaat ggcgacaata tcagaatagt cagcaccgct tccggctacc acttccgcta
    15961 ccacaccggc ctgagaagac catatctcag cgggatcatg ctccacccgg ccgggctgcg
    16021 gaaaaatctg ggtgaattcc ttctgggacg atgccacagg ctgcccgtca cttccgaaaa
    16081 ggatggccct tgagctggtg gttccctgat caagcgagag aatgtatctc ttcttcatac
    16141 tgtatgctcc tgtacgagaa atacagcagc taaagtacta ttttttgggt taatgccgtc
    16201 acagtcatcc ggctgtacct tacggaatat atacctgcac atggcagaaa attaatttta
    16261 aagcttaaat ttgagtatga taaaaccacc ggtcaatggt aaaccgatat attatcacta
    16321 taggaaggca gtttggcagc ggaggacggg agataggcca gaaactctct gccaggctgg
    16381 ggatagcgtt ttatgacaag gagctcatcc gtatcgcatc acaacagagc ggggtcaagg
    16441 aggagttctt cgagagagtt gatgagcaga agcatttcag cctcttcccg ggaatactgg
    16501 gactgcgcac ttcgctgacc gatgatttct tcccgaatta ctatctcagt aacgaatcgc
    16561 tgttcaggat acagagtgac gtgataagga ggctggctgg cgagggatca tgcatttttg
    16621 taggaaggtg tgcagattat gtgttaaagg aggaacggaa ctgcctgacc ctgtttatca
    16681 gcgctgacat ggcagaccga attaaacgga tcgcagtcag acacaagctt tcagaaagca
    16741 aggcgaaaga tttgatagag cgcactgaca aaggccgttc atcatattac aattatttca
    16801 gcgggaagac atggggtgcc gcagagtctt atcatctctg tataaattct tccctggccg
    16861 ggattgacga aaccgtcagg ctgataagcc atgtagccgg aatccgcttc ggactcacgg
    16921 aaaagaggga gtgattgtca taattgatca cctttcttaa aacggacact ctaattaatt
    16981 gataaggttt ctgttggcag aaacctgccg gcctgttatc ccggaggaga gatcagtaaa
    17041 aaagaagctg cctcaat
//
LOCUS       DAOPBK010000035        17036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3426_length_17036_cov_5.320358, whole genome shotgun sequence.
ACCESSION   DAOPBK010000035 DAOPBK010000000
VERSION     DAOPBK010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17036
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_17036_cov_5.320358"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLA17_03435"
     CDS             <1..560
                     /locus_tag="PLA17_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84496.1"
                     /translation="EEFYSYYGMKPSGNYSGHFSNAGEYLLTTDKSGNKILGFTFSDD
                     FPWPVEADGDGYSLVSSAADPSGNPGDATYWKRSMNKGGSPFADDPVSTAIERFINGR
                     RGELRIWPNPTSGMLVIDIDEEISGPMKLLFTSVTGATALVRDVEGTTLIDLSEHGLA
                     PGVYTVTAWHAGVSYMTKLIYIPER"
     gene            655..876
                     /locus_tag="PLA17_03440"
     CDS             655..876
                     /locus_tag="PLA17_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84497.1"
                     /translation="MDFKTGIGSSLKKMAFRIAGVVLMAYMLSFASCDTVPICWYCEN
                     PHNPTERQNVCNAMTKSKLESYGYVCTPY"
     gene            complement(895..2427)
                     /gene="aspA"
                     /locus_tag="PLA17_03445"
     CDS             complement(895..2427)
                     /gene="aspA"
                     /locus_tag="PLA17_03445"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HPK84498.1"
                     /translation="MNKNDILQFLKGIELLRNLTDGELNELAGHIHGKQCNAQYDSER
                     TRSENDLLGEREVPAEAYYGVQTLRAMENFNISAVTLNSFPSLIEALSMVKEAAAEAN
                     HELGLLDRSVRDAIVLTCHEIRNGRFHDQFVVDMIQGGAGTSTNMNANEVIANRALEM
                     LGYEKGDYAWCHPNNHVNLSQSTNDAYPTAVKIALYNLNRKLVAALETLVESFRRKGE
                     EFSHVLKMGRTQLQDAVPMTLGQEFGSYAVTLEEEIERLNMNAGPFLEINMGATAIGT
                     GINSEPGYSEKVTRHLRRITGLDLVLAPDLVKATQDTGAFVIYSSAVKCLAIKLSKIC
                     NDLRLLSSGPRTGIHEINLPPMQPGSSIMPGKVNPVIPEVVNQIAFKVMGNDLTVTMA
                     SEAGQLELNVFEPVMVHSLFESVELLINGMNTLRYRCIDGITANEEHCRSMVEKSIGL
                     VTALVPWIGYKSANELAAEALSSGESVYSLVLSKNLLSKEKLDQILVPENMLRPGKMK
                     DE"
     gene            2504..3283
                     /locus_tag="PLA17_03450"
     CDS             2504..3283
                     /locus_tag="PLA17_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPK84499.1"
                     /translation="MTEFDRFYYLGTLYLLNYPAMNTCLKTVCLAMVLMLFGPGMSGQ
                     NQIPFNGPWEDEKFFNAGYTLGFNVMTFDIDPSEHFTLTDSVYPARGSFSPGLNIHLS
                     LNFRLNQFFDIRLLPGISFGQRNVKYSSDRVHDTLFTPQKVESSFIEVPLQLKYGWRM
                     QNVKPYVVGGINYRYDFYAQKKYRLERPVYLRLNKPDLYYEAGAGVSFYLSEIRFALE
                     LKMSNGIADILAHDPHPDYPEYCNTIEKLKSRIWVLSLHFE"
     gene            3400..4122
                     /locus_tag="PLA17_03455"
     CDS             3400..4122
                     /locus_tag="PLA17_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPK84500.1"
                     /translation="MSFINEIWAGDGKTAGYKIPAEQLTITMRYNYKTLFASQHEKFT
                     AILGQDYFSSLLMEDDLSDSAIIISDKRVYQIGRLYESRSRIYGGGLRASRGKKIVSL
                     LDITGTACKEITRPVPGGALIAFGLLAAILGILSDVYQSTYIMLVIAAVSIAAGIWLI
                     TKLRKRYLVIEYAGGSMVHPVKFITDTELNLFQGIIADEKEKLISGTGEFKECPNCGE
                     KIQPVAKICRFCGEAQQEPGTE"
     gene            4386..6554
                     /locus_tag="PLA17_03460"
     CDS             4386..6554
                     /locus_tag="PLA17_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPK84501.1"
                     /translation="MEKRNITRTVSLALLLLTAFALPAGIGRDDGSTPPNLSDSSMML
                     MQVADRDFGDAPASYGSVDHLIDGRIYLGSVPPDAEASQHYSDEADGDDLNGIDDEDG
                     VKIPDMVQGGKATIQYYVTINPYSSISRYVYFSAWIDWNGDGDFNDSGERVATNVLRL
                     RSGTYSLDVTVPQNAVASRPTFARFRLGPRVDSPAGSANFGEVEDYIVNIRCANPPAP
                     RVGRITQPSCHSPTGSVVLENLPGTGVWTLTRVQDGDLVTGSGTSYTVTGLMPGTWNY
                     TVTNDGGCTSGPSANISIVQGPDIPGAPVVGTITQPSCNVSTGSLVLTGLPGTGNWTL
                     TRQPDGATFHGSGTSTTITGIQPGTYTFTVTNSIGCTSLPSQEVTIDPQPSIPTAPVP
                     GAITHPTCDLPTGSVVLSGLPATGTWTLTRFPGSININASGTTFNVIGLNPGSYNFTV
                     TNAAGCTSPVSADVHINPQPGPVPTLVINNPDPVCSPGVVDLTRPAVTAGSTPNLTYT
                     YWMDVHATDPLNTPSAAPEGTYYIRGTIPGGCSAVSPVTVTAYRQPTANAGVDQELTF
                     VFTTTLDAAEPDENSTGTWSVLNGTGTFANPNSAKTTVTDLSMGENLLLWTVSNNVCP
                     PATDEMLVTVKKVVIPTLITPDMDHKNDYFVIRDKAVLGRVELTVFDRRGALVFENLN
                     YDNLWHGTNYNGEPLPDDIYFYVIKTENGLSLSGYLYIRR"
     gene            complement(6639..8105)
                     /locus_tag="PLA17_03465"
     CDS             complement(6639..8105)
                     /locus_tag="PLA17_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK84502.1"
                     /translation="MKKITIILMVLGLMLITSCEDFLDVKPSNYAASETSITNAADAK
                     VAINGLMRKMTSSSYYGRNFVIYGDAKGGDFAIRSQGRGLDYYYSFNHSATSGSGSGF
                     WEQIYNCILQANNLIMNIEMMEAEDRGSAALSEYKGMALTARALMYFDLVRLYGKMYN
                     MEKSSYGVPLVLEPLDASAQPTRASVEEIYTQVLSDLTAAAPLLSKKKANGYINYYAN
                     KAIEARVHLFMDNFSASLAAAEEVINSGVYSLYSNDAWVGSWATQFGSESIFELGVYP
                     AEGDLRTGSLGYYLLRLGKVTGAMGWFMASDYWIDRMNQDPTDIRWAVMDYDESSDTR
                     FGSCLKYVGGSSMNGDKASASAVNIKVIRLSEMYLIAAEAALKLPTSDKARAAGYLNQ
                     IRKRSPGLAEATEETVTADMILDEKSKEFFAEGLRYFDMLRLNKTIEFNDEFIYPAVV
                     ITHREKTLDRTFYKTILPIDKGEIDANPAIGSQQNPGY"
     gene            complement(8124..11255)
                     /locus_tag="PLA17_03470"
     CDS             complement(8124..11255)
                     /locus_tag="PLA17_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK84503.1"
                     /translation="MKKLIFIFLSVLLVNAGMAQTRVTGKVTDSEDGSPVPYAVVVIK
                     GLRGVGTNTDADGNYSLSNVPPDAVLVFSYVGYKTQEIPVSGRSVINVEMVTDALALD
                     EVIVVAYGTSTKGTFTGAASVVKADAIKDVPTLSFENAMGGKIAGMQITTLSGQAGST
                     SSIRVRGVGSMNASNEPLYVIDGVPVISGNVTQMGIYTSSNVMSSINPNDIESITVLK
                     DAAASALYGSRAANGVIVITTKQGKQGKPVINFKASVGITPSFATDNYEVASTEQQVE
                     LYYENFWNAGVLYDGMTSEEASASALAQLNKRFNKHGYTFSAPDHTVNSLTIGGDRAG
                     QYFDWEDVLFRTAVYQAYDLSVSGGTEASNYYTSFSYTNDLGRSVINAFDRITGRMNF
                     GQKIGKYIELNTNINVSSSNKEGFNDSRALGNNYFLQSRNLLWGLYWPTDYGTGLPWT
                     ARYGSYAYNPVYYNNEWENWSKTQRVFANETVTIKILPELILKSIFSFDNTQTLDHLY
                     YSALHFSGTATQGSVSENSTKISELVSSTTLNYSKTFGGVHNVSALVGWEVAKNQTDY
                     QRSTGTNLPTSALHTVATAGKLDASAYYWGHTLASMLSRAEYNYDNKYYASASFRRDG
                     SSKLGPDSRWGNFWSVAGSWRIDQESFMDALSVISSLRLRASYGVNGTLPTSNYGWRS
                     LTSYANKYMEQPGSSVSTIADADLSWETSYTSNIALEFGLFSQRLTGSLEYFNRDSKN
                     LLQDVPLSRVTGFYTTLDNVGEINNRGLEIELYGEIMRAGDFTWSAGLTASHIKSTVT
                     RLYGGQDIIWYEPTGDDNRTKFIYTEGQSTLAIYGLEWAGVERETGLNLWFLNNDKEP
                     DLMVDGRPATYNYKNASEIVLGDAHPKLFGGYNTDLAWKGISLSLNFIYKLGGYTYDA
                     PGRDINDDGYYWERIMSLDAYENRWTPLNKDARYPQRVAIDMEDVNQKSSRHMHPADF
                     TRLKSITLAYNLPKDLLSKIRVSGARVYFNGANLWTLAAWKVYDPEVNEYGSRGWEMP
                     IGKTYTFGIDVSF"
     gene            11580..13244
                     /locus_tag="PLA17_03475"
     CDS             11580..13244
                     /locus_tag="PLA17_03475"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HPK84504.1"
                     /translation="MKLFKAFIPVMLLFFGCNATDTYDVLIRNGNIADGSGSPAFRGD
                     IGIMSDTIAAIGDLRKARGKTEIDANGMTVAPGFINMLSWAVESLIEDGRSMGDIVQG
                     VTLEVLGEGDSWGPWSESMKREFKASQSDIKYDIEWTTLGEYLEYLEKKGVSTNVASF
                     VGTATLRIHTVGYDNRPPSLEEMDSMKLLVKQAMEEGAIGVSSALEYVPASFATTGEL
                     TELCRVAAEYDGMYISHIRNEDDTFLEAIDDFLKIPAATGIRSEIYHFKQVGRTNWHK
                     LDQAVAKIDSARKAGLHVTADMYNYPASGTGLGIIMPEWVQEGGFDKWVARLRDPKVR
                     KVIGPEIMKTILRKQGTPEKIIVIGFDTDSLKYLQGKTVAEIAALRGTSPEETIMDLI
                     IDDGSNVSAVFFSMSEDNLRKEIAIPWMSFGSDGKSMAPEGVFLKSATHPRAYGNFAR
                     LLGRYVRDEKVIPLEKAVYRLTTLPAANMKIEKRGALRVGYYADVVVFDLENIQDHST
                     FEDPHQLSTGMSHVFVNGVQVLRDGVHTGATPGRVVRGPGFRTEAR"
     gene            complement(13332..13580)
                     /locus_tag="PLA17_03480"
     CDS             complement(13332..13580)
                     /locus_tag="PLA17_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPK84505.1"
                     /translation="MEIVVSLLIGAVAGWLGSVIYKGSGLGLPGNIIVGLIGGPVGYW
                     ILGKLGITLGGGWFGAILTGAIGAIIILFLINLVVRKK"
     gene            complement(13635..15419)
                     /locus_tag="PLA17_03485"
     CDS             complement(13635..15419)
                     /locus_tag="PLA17_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84506.1"
                     /translation="MMMKAFLLTVILAALAAAGVSQPLPQFKLTGTFGEQQMVIENGV
                     AGTRVLINAPVTGFGKEDRVLLVLYALPNGNTIEQTFGKKLKEGDDWHYDIQHIGAQT
                     RFLRRMITDRTVVVACLENEVKSWPAWSANTPDYKEIVKKMVDEITSLFAPWNPELVL
                     NGHSGGGRFIFNYLDAHEQIPGSVVRIAFLDSNYGWEDDRYGPKIVNWLRSGRNRYFC
                     TLAYNDSVVVYNGKPLVSPEGGTWYRSRKMNDYLSASFRLKRYERDSLIWYSSRDRRI
                     VFIFKPNPEGKILHTQQVEYNGFVHSMLSGTRLEQRGYRYFGMRAYQDLISDTVTLPI
                     RRLNIPPRDHSSVTGSEFMKRINDLSREEREKEIYREVASGNIPGFLRETVTLRAVFH
                     DLNDIPHMLEYEVMPDYLAIGSDDDYCRIPMSPGTARRLADLFGASLLTAKLSDHIWS
                     VAEVKPEPFFYRPVGNENEKAAKFFEHNEQIERQLADAGWRPGQLVAGIKKDVIISSH
                     IADRPDRVVIYGWHRHDGSPIQPVYSGHIWWYVDYSHGIRFMNAQVLVDGSPVKVSDL
                     LKDPVLFRIVTDEENPLRLSFYPENMIL"
     gene            15577..>17036
                     /locus_tag="PLA17_03490"
     CDS             15577..>17036
                     /locus_tag="PLA17_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK84507.1"
                     /translation="MSEEILRAMMELFALIVKQDGGMLQSERDFVSAFLTKQLPHQSA
                     DEFMHLFLENAGPLGQKGKKPMSGSASVKDSVRILNNCNKINKTLSQEQRVIVLMRCF
                     ELIDSGRQYTPQRMNIINTIAEVFRISTSEFNSIMQFVREDDRAGFSDQSMIVIDTGR
                     VAYNKPGPESFIVFLKVASVNLYFMKCFCDGNTLLNGLPLVCRRVYLFAPGSSVQAPP
                     APSIYYSDIISAFAAGKDIHRLSFVAEKISYSFPDRTPAITDVSFAAGEGMLIGIMGA
                     SGSGKTTLLNLLTGLVKPESGEIRINSIPIYTNDKRLDGVIGYVPQDDILIEDLTVFE
                     NLFYAASLCFAGRKREELTADVNKTLQSLGLYEKKDYRVGSPLNKVISGGQRKRLNIA
                     LELIREPSVLFLDEPTSGLSSADSENVMDLLHELTVRGKLVITVIHQPSSDIFKGFDK
                     VLILDMMGHLVFYGNPLEAIVHFKTLESHINCGVAE"
BASE COUNT         4366 a         4312 c         4069 g         4289 t
ORIGIN      
        1 ccgaagagtt ttattcatat tatggtatga aaccaagcgg aaactacagt ggacattttt
       61 cgaacgccgg agaatacctg ctgacaacag ataaaagtgg taacaagatt cttgggttca
      121 ccttctcgga tgacttcccc tggcccgtag aggcagacgg cgacgggtat tcactggtat
      181 caagtgctgc agatccgtcc ggtaatccgg gtgacgccac ctactggaag cgctcgatga
      241 acaagggagg gtctcctttt gccgatgacc ccgtatccac ggcaatagaa agatttatca
      301 atggcaggag gggcgaacta cggatctggc ccaacccgac aagcggcatg ttagtgatag
      361 atattgacga ggagatcagc ggcccgatga aactcctgtt cacatctgtt accggtgcaa
      421 cggctctcgt acgcgatgtt gaaggcacaa cactgattga cctgtcagag catgggctcg
      481 cgccgggagt ttacaccgtt accgcctggc acgccggggt ttcatacatg acaaaactta
      541 tatacattcc cgagaggtag tgatctccat gaacagcgat caggctgatc tgtaaccccg
      601 gaacctgaaa aaatgatttt gaaatttgct cagtacctga ttaactttgc ataaatggat
      661 tttaaaaccg gtataggatc atccttgaag aaaatggcct tccgcatagc gggagttgtg
      721 ctgatggcat acatgctgag ctttgcttca tgcgatacgg tacctatctg ctggtattgc
      781 gagaatccgc ataacccgac tgagcgacag aacgtttgta acgccatgac caaaagcaag
      841 cttgagtcat acggttatgt ctgtactccg tactgatctt ccggatgtgc ccggtcactc
      901 atccttcatc tttcccggcc tcaacatgtt ttcagggaca agaatctgat caagtttttc
      961 ctttgacagc agatttttgc tgagcaccag tgaatatacg ctttcacccg atgaaagggc
     1021 ttccgctgcc agttcattgg ccgacttgta tcctatccag ggcaccagtg ccgtcaccag
     1081 tccgatgctc ttctcaacca tagaccggca gtgttcctca ttggcagtga taccatcaat
     1141 gcacctgtat ctcagtgtgt tcattccgtt aatcagcagc tcaaccgact caaagagact
     1201 gtgaaccatc actggttcaa agacattgag ttcaagctgg ccggcttccg aagccatggt
     1261 aaccgtaagg tcatttccca tcaccttgaa tgcaatctgg ttgaccactt cgggaattac
     1321 cgggttgacc ttcccgggca tgattgatga cccgggctgc atcgggggaa ggttgatctc
     1381 atggatacct gtcctggggc ccgaggagag caaacggaga tcattacaga tcttcgacag
     1441 cttaatggcc agacacttca cggcagatga atatatgaca aatgcccctg tatcctgtgt
     1501 tgccttcaca aggtcgggag caagtaccag gtcaagaccg gtgatcctcc tcagatgccg
     1561 ggttaccttt tctgaatagc cgggctccga attgatgcct gtaccgattg ccgtggcacc
     1621 catgttaatc tcaagaaaag gacctgcatt catgttcagt ctctcaatct cctcctccag
     1681 ggtgacggca tatgacccga actcctgacc cagggtcatg ggaacggcat cctgcaactg
     1741 ggtgcgcccc atcttgagaa catgtgaaaa ctcctctcct ttgcggcgga atgactcaac
     1801 aagggtttca agcgccgcca ccagtttccg gtttaagtta tagagcgcta tcttcaccgc
     1861 ggtgggatag gcatcatttg tagactgaga aagattcaca tgattgttcg ggtggcacca
     1921 ggcatagtcg cccttctcat atcccagcat ctcaagcgcc ctgtttgcga tcacctcatt
     1981 ggcattcata tttgttgacg tgccggcccc gccctgaatc atatcaacca caaactggtc
     2041 atgaaatctg ccgttccgga tttcgtggca tgtcagaaca attgcatccc tgacgctccg
     2101 gtcaagcagc cccagctcat ggtttgcctc tgcagccgct tccttaacca ttgagagtgc
     2161 ctcaatgagt gatggaaaag agttcagcgt cacagctgag atattgaaat tttccatagc
     2221 cctgagtgtc tgcacgccat aataagcctc agccggcacc tcacgctcac ccaggaggtc
     2281 attttcagat cgtgtgcgtt ctgaatcata ctgtgcattg cattgcttcc cgtggatgtg
     2341 tccggcaagc tcgttcagtt ccccgtccgt cagattcctt aagagctcaa tccccttcag
     2401 gaattgtaaa atgtcgttct tattcatggt tcaactatcc cttaatcagt aaacggcttc
     2461 aaggtctaat aattttaaat aattattaat actaaaaatc agcatgaccg aattcgatcg
     2521 gttttactat cttggtaccc tatacctgtt gaattatccg gcaatgaata cctgtctgaa
     2581 aacagtttgt cttgcgatgg ttttgatgct gttcgggcct ggcatgtcag gacagaatca
     2641 gatcccgttt aacggcccat gggaggatga aaagtttttc aatgcaggat acaccctcgg
     2701 attcaatgtg atgactttcg acattgatcc ctcggagcat tttaccctca ccgattcagt
     2761 ttatccggcc aggggatcat tctctcccgg gctgaatata cacctgtcgt tgaatttcag
     2821 gctgaaccag ttttttgata taaggctcct gcccggcata tctttcggac agcgaaatgt
     2881 gaaatattca tctgaccggg tacatgacac gctctttact ccgcaaaagg ttgaatcgag
     2941 tttcattgag gtccccctgc aactgaagta cggctggagg atgcagaatg tcaagcctta
     3001 tgttgttggt ggcatcaatt acaggtatga tttctacgcc cagaaaaaat accgcctgga
     3061 gcgtcctgtt tacctgaggc tgaacaaacc agacctgtac tatgaggctg gtgcgggggt
     3121 cagtttttac ctgagtgaaa taaggttcgc cctggaactg aagatgtcaa acggtattgc
     3181 agatatactt gcccatgacc ctcatcctga ctatcctgaa tactgcaata cgattgagaa
     3241 actaaagtca cgaatctggg tgttgtctct tcattttgaa taagtgcggc aggagtaata
     3301 gagctgcaga cgggtaatct aagaacttag tccattaatc ctctttgtgc cattgttggt
     3361 caaaattatc ttctatttca ggcctgtagc gcttacacta tgagctttat taacgaaatt
     3421 tgggcaggcg atggtaaaac tgcaggctac aagatacctg ctgaacaact gacaataacg
     3481 atgagataca actacaaaac cctctttgcg tcgcagcacg aaaagtttac tgcgatattg
     3541 ggtcaggatt atttctcgtc gcttctgatg gaagatgatc tgtcagattc tgccattatc
     3601 atttcagaca agagggttta ccagataggc cggttgtatg agtcacgttc ccgcatttac
     3661 ggcggaggac taagggccag caggggaaag aagattgtca gtctcctgga tattacaggc
     3721 actgcctgca aggagataac cagacccgtt cccggaggag ccctgatagc atttggcctg
     3781 ctggcagcca ttctcggcat tctctctgat gtgtaccagt caacttatat aatgctggtc
     3841 atcgcagccg tgtcaattgc cgcaggcatc tggctcatca caaagctcag gaaaagatac
     3901 cttgtcattg aatatgccgg aggaagcatg gtgcatccgg taaaattcat tacagacacc
     3961 gaactcaacc ttttccaggg catcattgcc gatgagaagg agaaactgat ttcagggacc
     4021 ggggaattca aggagtgtcc caactgcggt gagaaaatac agcctgttgc aaagatatgc
     4081 cgtttctgcg gcgaagccca gcaggagcct ggtacagagt aaccacgcca gggatcagcc
     4141 ggattttacc atgtcataat taccgtttat tgatcaatta atttcattta ttcgtcaaat
     4201 cggaatttat gtcgtacagc atgaacacat ggtgtgagag gccggaaaaa tgcaagtacg
     4261 cgcaacttca gcccggaaca gggataatct gccaatcaaa ttatctttgc atataccttt
     4321 ctgagtctct aacttgtagc atttcaggtc cggtaactgt caaccgggtc tgttaaaaaa
     4381 gcagaatgga aaaaagaaac ataacacgaa cagtctccct tgcattgctc cttctgacag
     4441 cctttgccct cccggcggga ataggaaggg atgatggttc cacccctcca aacctgtctg
     4501 actcatccat gatgctcatg caggttgctg accgtgactt tggggatgcc ccggccagct
     4561 acggatcagt tgatcatctg attgacggac gcatctatct tggctctgtg ccgccggatg
     4621 ctgaagcttc acagcactac tctgacgagg cagacggtga tgacctgaac ggaattgacg
     4681 acgaggacgg agtgaagatt cccgacatgg tacagggggg taaagctact attcagtatt
     4741 atgtgacgat aaacccgtac tcctccatat cgagatatgt ttacttcagc gcctggatcg
     4801 actggaacgg agacggagac ttcaatgact cgggagagag ggtggcaacc aatgtgctga
     4861 gattaaggag tggcacctac agcctggatg taactgtgcc tcagaatgct gtagcatcca
     4921 gacccacctt cgcaaggttc aggctgggcc cgcgcgttga ttctcctgcc ggttcggcaa
     4981 atttcggtga agtggaggat tatattgtga atatcagatg tgcaaatcct ccggctccgc
     5041 gggtaggccg tatcacgcaa ccttcctgcc attccccgac cggcagcgtg gtgctcgaaa
     5101 acctgcccgg aacaggtgta tggaccctca cacgcgttca ggacggagat ctggtgacag
     5161 gcagcggtac atcatatact gttacgggtc tgatgccagg cacatggaac tatacggtta
     5221 ccaatgatgg aggctgcact tccggtccgt cggcaaacat aagcatcgta cagggccctg
     5281 acatacccgg tgccccggtg gtgggaacaa ttacccagcc ttcgtgcaat gtctccaccg
     5341 gaagccttgt cctcaccgga ctgcccggca caggtaactg gacgctgacc aggcagcctg
     5401 acggagccac attccatgga tccggtacaa gcacaaccat aaccggaatt cagcccggca
     5461 catatacatt tacggtcacc aattccatcg gctgcacctc attgccgtca caggaggtta
     5521 caattgatcc gcaaccttcc atccccaccg caccggtgcc gggagcaatc acacacccca
     5581 catgcgacct tcccacggga agtgttgtct taagcggcct ccccgctaca ggcacatgga
     5641 cactcacccg tttccccgga agtatcaaca tcaacgcctc gggcaccact tttaatgtaa
     5701 tcgggctgaa tcccggttca tataacttta ccgtgaccaa tgccgccggc tgtacatcac
     5761 cggtatctgc tgatgttcac atcaatcccc agcccggtcc cgtaccaacg ctggtaatca
     5821 ataatcctga tccggtctgt tcaccgggtg tcgttgatct gacgcggccg gccgtaactg
     5881 caggatctac accaaacctc acctatacct actggatgga tgttcatgct actgatcctc
     5941 tgaacacgcc gtcagcagcc cctgaaggga cctactacat caggggtaca atccccggtg
     6001 gttgttccgc cgtgagcccg gtaaccgtta cggcataccg gcaacccaca gcaaatgcag
     6061 gagtagacca ggagctgaca tttgtcttca ccaccaccct tgacgctgcc gaacctgatg
     6121 agaattccac gggaacatgg tctgtgctga atggtaccgg aactttcgca aatccgaatt
     6181 ctgcaaaaac aaccgtgaca gatctctcca tgggcgaaaa cctcctgtta tggaccgtat
     6241 cgaacaatgt ctgtccacct gcaacggatg agatgcttgt cactgtgaag aaagtagtga
     6301 tcccaacgct gattaccccc gatatggatc ataaaaatga ctatttcgta atcagggata
     6361 aagctgttct cggcagggtt gaactgactg tgtttgacag aaggggagcg ctggtttttg
     6421 agaatctcaa ctatgacaat ctctggcacg gaacaaacta caatggtgaa cccctgcctg
     6481 atgacattta tttttatgtc ataaaaactg agaacggtct ttcactgagt ggttatctct
     6541 atatcagaag gtagattcac tcatataaat gaaaaatcaa ggccgcccac aaacggggcg
     6601 gcctccttat ttgcataaag ccttactaca gccaggtatc agtaaccggg attctgctga
     6661 ctgcctattg ccgggttggc atcaatctca cccttatcta taggcaggat agtcttgtag
     6721 aatgtcctgt caagggtctt ctcacggtgt gttattacaa ctgccggata gatgaactca
     6781 tcgttgaact caatggtctt gttgagcctg agcatgtcaa aatacctgag cccttcggca
     6841 aagaactcct tgctcttctc atcaaggatc atgtcagcag tcacagtttc ttcagtagcc
     6901 tctgccaggc ccggagatct cttccttatc tgattaagat aacctgcagc cctggctttg
     6961 tcagacgtcg gaagcttcag ggcagcttcg gcagcaatga ggtacatctc ggaaagccga
     7021 atcaccttaa tgtttactgc cgaggctgaa gctttgtcac cattcattga tgatcctcca
     7081 acatacttaa ggcatgaacc aaagcgcgtg tcagaagatt catcgtagtc catcactgcc
     7141 cagcgaatgt cagtcgggtc ctggttcatc ctgtcaatcc agtaatcact tgccatgaac
     7201 caccccattg ctcctgtcac cttgccgagg cgaagcagat agtacccgag ggaaccggtt
     7261 ctaaggtctc cttcagcagg atatacgccc agttcaaaga tcgattccga accgaattgt
     7321 gtggcccagg aaccaaccca ggcatcattt gagtagagtg aataaacccc gctgttgatg
     7381 acctcttcgg cagcagccag actggcgctg aagttatcca tgaagaggtg cacgcgtgct
     7441 tcgatggcct tgttggcata gtagttaatg tagccgtttg cctttttctt cgagagaagc
     7501 ggtgccgcag cggtcaggtc actcagtacc tgggtatata tctcctcaac cgatgctctt
     7561 gttggctggg ctgaggcatc aaggggttca agcaccaggg gaacaccgta tgatgatttt
     7621 tccatgttgt acattttgcc gtacagcctg accaggtcaa aatacatcag tgcccttgcg
     7681 gtcaatgcca ttcccttata ctcgctgaga gcagcgcttc ccctatcttc tgcctccatc
     7741 atttcgatgt tcataatcag gttgttggcc tgcaggatac agttgtatat ctgctcccag
     7801 aaccctgaac cggaacctga tgtggcagaa tggttgaatg aatagtagta atcaaggccc
     7861 ctgccctgtg aacgaatggc aaaatcgccc cccttggcat cgccatatat tacgaagttc
     7921 cgcccgtaat aggacgaaga agtcatcttt ctcatcagcc cgttgatggc caccttggcg
     7981 tcagcggcat ttgtgatgga agtttccgaa gccgcgtagt tgctgggctt gacatcgagg
     8041 aaatcctcgc atgaggtgat cagcatgagg cccagaacca taagtattat agttatcttt
     8101 ttcatcgttg tgccttttta ggattagaaa ctgacatcaa tgccgaaagt ataagtcttg
     8161 ccaattggca tttcccagcc tctggagccg tattcattaa cttccggatc atacaccttc
     8221 caggcagcaa gtgtccagag gttggctccg ttgaagtaga ccctggcccc tgatactctg
     8281 atcttgctca gaagatcttt tggcaggttg taagcaagag taatgctctt caggcgggtg
     8341 aagtcggccg ggtgcatgtg ccggctgctc ttctggttta catcttccat gtcaatggcc
     8401 acacgctgag gataacgcgc atccttgttg agtggtgtcc accggttttc ataggcgtca
     8461 agtgacatta tcctttccca gtagtagccg tcgtcattga tatccctgcc cggagcatcg
     8521 taggtgtaac cgccaagctt gtaaatgaag ttgagcgaaa gcgaaatacc cttccaggcc
     8581 agatcggtat tataacctcc gaatagtttc ggatgggcat ctccaagaac tatttcgctg
     8641 gcattcttgt agttgtatgt ggcaggcctg ccgtcaacca tcaggtcagg ttccttatca
     8701 ttattcagga accacagatt gagcccggtc tcccgttcaa caccggccca ctcaagtccg
     8761 tagatggcca gcgtcgattg accttcagtg taaataaatt ttgtccgatt atcatcgccg
     8821 gtaggttcat accatattat atcctgccct ccatacaacc tggtcacagt tgacttaata
     8881 tgcgaggctg taagcccggc actccaggtg aaatctcctg ctctcattat ctcaccgtag
     8941 agttcaattt ccagtccgcg gttgtttatc tcgccaacat tatcaagcgt tgtataaaaa
     9001 cctgtcaccc ttgagagagg aacatcctgg agaaggtttt tcgaatctct gttaaagtac
     9061 tccagtgaac cggtcagtct ctggctgaat aacccgaatt caagtgcaat gttggaggtg
     9121 tagcttgtct cccatgacag gtctgcatct gcaatggtac ttacactgct gccgggctgc
     9181 tccatgtatt tgttggcata gctggtaagt gagcgccatc cgtagttgct ggttggcaga
     9241 gtaccgttaa caccgtatga tgctcttagc ctcaggctgc tgatgacaga cagagcatcc
     9301 atgaagctct cctgatctat tctccatgat ccggctactg accagaagtt gccccagcgt
     9361 gaatcagggc caagctttga tgagccgtca cggcggaatg aagccgaagc ataatacttg
     9421 ttgtcatagt tgtactcggc ccgtgagagc attgaagcca acgtatggcc ccagtagtag
     9481 gcgctcgcat caagcttgcc tgccgtggct acggtatgaa gggcgctggt gggaagattt
     9541 gtgccggtag atctctggta gtcagtctgg ttttttgcaa cctcccagcc aaccagggca
     9601 cttacattat gtactccgcc aaaagtcttg ctgtagttga gagtggttga agagacaagc
     9661 tcactgatct ttgtgctgtt ctcggaaacg gaaccctggg tggctgtgcc actgaaatgc
     9721 agagcactgt aatacaggtg atcaagggtc tgggtattgt caaatgagaa gatagacttg
     9781 agaatcagct caggaagtat ctttatggta acagtctcat tggcaaatac ccgctgggtc
     9841 tttgaccagt tttcccactc attgttgtag taaaccggat tatatgcata gcttccatac
     9901 ctggctgtcc agggcaaccc cgtaccatag tcagtcggcc agtagagccc ccacagcagg
     9961 ttacggctct gcaggaagta gttgtttccc agggcacggc tgtcattgaa accctctttg
    10021 ttactgctgc tgacattgat atttgtgttc agctcaatat atttccctat cttctgaccg
    10081 aaattcattc ttcccgttat acggtcaaag gcgttgatga cactgcggcc gaggtcattt
    10141 gtataggaga atgaagtgta gtaatttgaa gcctctgtac caccactgac agagagatca
    10201 taagcctggt acaccgctgt gcggaaaaga acatcctccc agtcgaaata ctgaccagct
    10261 ctatcacctc ctatcgtaag cgaattgacc gtatggtcag gagccgagaa ggtataacca
    10321 tgcttgttga acctcttgtt cagctgtgca agggcactgg cactggcctc ttcactggtc
    10381 atgccgtcat aaagaacacc tgcattccag aagttctcat aatagagctc cacctgctgt
    10441 tcagtgctgg caacctcgta gttgtcagtt gcaaaagagg gggttatacc cactgaagcc
    10501 ttgaaattaa tcacaggctt gccctgctta ccctgttttg tggtgatgac aattacaccg
    10561 ttggcagctc ttgacccgta aagggctgag gcagcagcgt ctttcagaac ggtgattgac
    10621 tcaatgtcat ttggattaat cgagctcatc acattactgg aggtgtaaat acccatctgt
    10681 gtgacgttac ccgatatcac cggaacacca tcaataacat aaagaggctc attggaagca
    10741 ttcatggaac ctacgccgcg caccctgatc gaggaggtgg aaccagcctg ccctgaaagg
    10801 gttgtaatct gcatgccggc gatctttcca cccatggcat tctcaaatga aagcgtcggg
    10861 acatccttga tggcatcagc cttcaccacg ctggcagcac cggtaaaggt tccctttgtg
    10921 gatgtaccgt aggcaacaac aatcacctca tcaagcgcaa gagcatcagt gaccatctca
    10981 acattgatga ccgatctccc tgaaaccggg atctcctgcg ttttgtaacc cacatagctg
    11041 aacaccagta cagcgtcggg aggaacattg ctgagagaat aatttccgtc agcatcagta
    11101 ttggttccaa ctccccgtag cccctttatc actactactg cataagggac cggagaacca
    11161 tcttcagaat cggttacctt cccggtaaca cgggtctgcg ccatgccggc attgactaac
    11221 aataccgaca ggaaaatgaa gattagtttt ttcatggaaa agagcgttag tttgattaaa
    11281 gttaactctc acaagttact gagaaattta ttcttattaa aaaaaattgt attacatcat
    11341 actgctttaa aaaacagagg tgaagcaaca ttttgaatga actgaaaaac gcattaaatg
    11401 ccattaccag gttaccggat atcatccgga agattccgaa cagaaaccat tctttccgga
    11461 aatttatgtt aaaaataata tctgcggagc caaaatgctc tttcaggctg cttaaaaatt
    11521 tttcagatag tcattttttt ttcacttttg agtagatttc actcaaatcc ttgttcaaaa
    11581 tgaaattatt caaagctttt atcccggtga tgctgctatt cttcggctgc aatgccactg
    11641 acacgtatga cgtactcatc cggaacggaa atattgcaga tggatcaggc tcgccagcct
    11701 tcaggggaga tattggtatt atgtctgaca ccattgctgc cattggtgac ctccggaagg
    11761 ccagaggcaa aacagagata gatgccaacg gaatgaccgt tgcaccaggg tttatcaaca
    11821 tgctcagctg ggcagtggaa tcactgatag aagatggcag gtcaatgggt gacatagttc
    11881 agggtgtgac ccttgaggta ctcggtgagg gtgactcatg gggcccctgg agtgaaagca
    11941 tgaagaggga atttaaggca tcacagagcg acataaaata tgatatagaa tggacaacgc
    12001 tgggtgaata tctcgaatat ctcgaaaaga agggggtatc aaccaatgtg gcatcttttg
    12061 tagggactgc aaccctcaga atccatactg taggatatga caaccgtccg ccatcactgg
    12121 aggagatgga ttcgatgaaa ctgctcgtaa agcaggccat ggaagagggc gccatcggtg
    12181 tcagttcggc cctggaatat gtccctgcca gcttcgccac caccggagag ctgaccgaac
    12241 tctgccgggt ggctgcagaa tacgacggaa tgtacatctc acatatcagg aacgaggatg
    12301 acaccttcct ggaagccatt gatgatttcc tgaaaattcc tgctgcaaca ggcatcaggt
    12361 ctgagatata ccacttcaag caggttgggc ggaccaactg gcacaagctt gatcaggccg
    12421 tggcgaagat tgattcggca cggaaggccg ggctccatgt cacggcagac atgtacaatt
    12481 acccggcaag cggtaccggg ctgggtatca tcatgcccga atgggtgcag gagggcggat
    12541 ttgacaaatg ggtcgcaagg ctccgtgatc cgaaggtaag aaaggtgatc ggaccggaaa
    12601 taatgaagac gatactgaga aaacagggta cacccgaaaa gattattgtc atcggattcg
    12661 atactgattc actgaaatac ctgcagggaa aaacagttgc cgaaatagcc gccttgaggg
    12721 gcacatcacc ggaggagacc atcatggacc tcatcatcga tgacggaagc aatgtatcgg
    12781 ctgtattctt ttccatgtca gaggataacc tcaggaagga gattgccatt ccctggatga
    12841 gcttcggttc agatggcaaa tcgatggcac cggagggcgt ttttctgaaa tcggctaccc
    12901 atccgagggc atacggcaat ttcgcccggt tgctgggcag gtatgtaagg gatgaaaagg
    12961 tcatcccgct ggagaaagct gtctacaggc ttaccaccct tccggctgcc aatatgaaaa
    13021 ttgaaaagcg tggtgccctc agggtaggtt actatgctga tgtggttgtt tttgacctgg
    13081 agaatattca ggaccactca acctttgagg acccgcacca gctctcaacc ggcatgtcac
    13141 atgtttttgt aaacggagtc caggtgctcc gtgatggagt ccataccggc gccaccccgg
    13201 gcagggttgt acgtggcccg gggttccgga cggaagccag ataacgcttg ctgaggttca
    13261 ctgtttttaa aagtttcggc aaacagatgt aataatctgc cgaccttgtc tagaataaag
    13321 agactgactc tctatttctt cctgacaaca aggtttataa ggaagaggat aattattgca
    13381 ccgattgcac ccgtcagaat tgcaccgaac cagccgccgc caagggtaat accaagcttg
    13441 ccaagaatcc agtaacccac cgggccccct atcagaccca cgataatgtt acccggcaga
    13501 cccagaccgc tgcccttgta aattacactg cccagccagc ctgctacagc tccgataaga
    13561 agtgaaacca ctatttccat ctcaatcagt tttaggattt aactttaaag gcccggaaat
    13621 ttattgaata gaatctacaa tatcatattc tcaggataaa aagagagcct gagaggattt
    13681 tcctcatcag tgacaatcct gaaaagcacc ggatctttca ggagatcaga gactttaaca
    13741 ggactgccgt caaccagtac ctgagcgttc ataaacctta ttccatggct gtaatcaaca
    13801 taccaccaga tatggccgct gtatacaggc tggatggggc ttccgtcatg tcggtgccag
    13861 ccgtatatca ccaccctgtc aggccggtct gcaatatgtg atgaaataat aacatccttc
    13921 tttatacctg ccacaagctg accgggcctc cagccggcat cagccagttg cctctcaatt
    13981 tgttcattgt gttcaaaaaa cttcgccgcc ttttcatttt cattaccgac aggtctgtaa
    14041 aagaacggct caggcttcac ttcagctaca gaccatatat gatcactcaa ttttgctgtc
    14101 agcaatgaag caccaaacag gtcagccagc cgccgggctg ttcccggact catgggaatc
    14161 cggcagtaat catcatcact ccctatggcc aggtaatcag gcattacctc atattccagc
    14221 atatgaggaa tatcgttcag atcatggaac actgccctga gggtaacggt ttcacgcagg
    14281 aaaccaggaa tattcccact ggcaacttcc cggtatattt ctttctcccg ttcctcccgg
    14341 gaaagatcat tgattcgctt catgaactct gaaccggtaa cggatgagtg atcacggggc
    14401 ggaatattca gcctccggat ggggagagtg acggtgtctg atatcagatc ctggtaagcc
    14461 cgcatcccaa agtacctgta tccccgctgt tccagtctgg tgccactcag catactatga
    14521 acgaatccgt tatactccac ctgctgagta tgaagtatct tcccttccgg gtttggcttg
    14581 aagatgaata caatcctcct gtcacgtgac gaataccaga tcagtgagtc acgttcatac
    14641 ctcttcagac ggaacgatgc agagaggtaa tcattcatct tcctgctccg gtaccatgtg
    14701 cctccttcag gtgacaccag cggtttgccg ttgtaaacaa ccacgctgtc gttataggcc
    14761 agtgtgcaga aatacctgtt cctgcctgac cggagccagt tgacaatctt cggcccgtaa
    14821 cggtcatcct cccacccgta attgctgtcg aggaatgcaa tccgtacaac acttcccggt
    14881 atctgttcat gggcatcaag atagttgaaa ataaaccgcc cgccgccgct gtgtccgttc
    14941 agaaccagct caggattcca gggtgcgaac agggaggtga tttcatcaac catcttttta
    15001 acaatctcct tatagtcagg ggtattggca ctccatgccg gccagctctt cacttcattc
    15061 tcaagacagg ctaccaccac ggttctgtca gttatcatcc ttctcaggaa cctggtctgt
    15121 gcgcctatat gctggatgtc atagtgccag tcatcaccct ctttcagctt cttcccaaaa
    15181 gtctgttcaa tggtgttgcc gtttggaaga gcatagagta ccagcagtac ccggtcttcc
    15241 ttgccgaatc ctgtcacggg ggcgttgatg agcaccctgg tacctgcaac gccattttca
    15301 attaccatct gctgttcgcc gaaggtcccg gtgagtttga actgcggcag gggctgcgag
    15361 acacccgcag cagccaatgc tgccagaatc actgtcagaa ggaaagcttt catcatcata
    15421 cccgacatat aagaataaaa gagcggcatc cggcagatgc atggttataa aagtaagtaa
    15481 taaagattaa ggatatccat cattaacaat tagtccgcat ttattatccg attttattaa
    15541 cttgtccgtt atatgtgata taactgaccg gaaaagatga gtgaagagat tctgcgggcc
    15601 atgatggaat tgtttgccct gattgtcaag caggacgggg gcatgcttca gagtgaacgt
    15661 gattttgtct cagctttcct taccaaacag cttccgcacc agagcgcaga tgaattcatg
    15721 catctcttcc ttgaaaatgc cgggccactc gggcaaaagg gcaaaaagcc gatgtccggg
    15781 tcagcctcgg taaaagattc agtcagaatc ctcaacaact gtaataagat caacaaaacc
    15841 ctttcccagg aacaaagggt cattgtactc atgcgctgct ttgaactgat tgattccggc
    15901 aggcagtaca ctccacagcg tatgaatatc atcaacacca tcgctgaggt gttcaggata
    15961 tcaacgtctg aattcaacag tatcatgcag tttgtcaggg aagatgaccg ggcaggcttc
    16021 agtgaccagt cgatgattgt aatcgacacc gggcgggtgg catacaacaa acccggtcct
    16081 gaatccttca ttgtcttcct gaaggtggca agtgtcaacc tttacttcat gaaatgcttc
    16141 tgtgacggga acaccctcct taacgggctc cccctggttt gccgcagggt ctacctgttt
    16201 gccccgggaa gttctgtaca ggcgcctcct gcaccaagca tatattacag tgatatcatc
    16261 tcagcttttg ctgccggaaa ggatattcac aggctctctt tcgtggccga aaagatcagc
    16321 tattccttcc ctgacagaac tccggccata acagacgtca gctttgcagc gggcgaagga
    16381 atgctgatag ggatcatggg ggccagcgga tcgggaaaaa ccacactgct caacctgctg
    16441 acaggccttg taaaacctga atccggtgag atcaggataa acagcatccc gatttatacc
    16501 aacgacaaga gacttgatgg agtaatcggg tatgtgcccc aggatgatat cctgattgag
    16561 gatctgactg tctttgagaa tcttttctat gccgcatctc tctgttttgc cggcaggaaa
    16621 cgggaggagc tgacagcaga tgtaaataaa actcttcaga gcctgggcct ttacgaaaaa
    16681 aaggattacc gcgtaggatc accactcaac aaggtcatca gcggcggaca gaggaagagg
    16741 ctgaacatcg ccctcgagct gatccgcgaa ccatctgtcc tcttccttga tgaacctact
    16801 tcagggctct catcagccga ttctgaaaac gtgatggatc tcctacacga actgacagtc
    16861 aggggcaagc tggtaataac agtcattcac cagccttcat cagatatttt caagggattt
    16921 gacaaggtgt tgatccttga catgatgggc cacctggttt tctatgggaa cccgcttgaa
    16981 gcaattgtgc acttcaaaac ccttgagtca catatcaact gtggggtcgc cgaatg
//
LOCUS       DAOPBK010000036        16807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3481_length_16807_cov_5.108405, whole genome shotgun sequence.
ACCESSION   DAOPBK010000036 DAOPBK010000000
VERSION     DAOPBK010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_16807_cov_5.108405"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLA17_03495"
     CDS             <1..342
                     /locus_tag="PLA17_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPK84508.1"
                     /translation="QHALKLYLESHNMSATTEEGKRAVLTLIENEVNEYRINGKYGHM
                     FPHRWLPVALGVLNESFNEENGMMNSTMKIVRGKITDRYHDLIEWLYTPMGKIVHNER
                     NMEEVEKMKLG"
     gene            363..2189
                     /locus_tag="PLA17_03500"
     CDS             363..2189
                     /locus_tag="PLA17_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK84509.1"
                     /translation="MKYFLIPVLVVISGIMTCCHREKAPEWRPAENPIYTVWGEKLDP
                     SAPWPEYPRPALARDEWMNLNGLWDYAICPKDADRPEPQGKILVPFPVESALSGVRER
                     VNDSLALWYSRDFVLPKQWKDKRIIINFEASDWETTVWVNDKLATVHRGGYDPFWCDI
                     TNLLDGRKKHTLVVRVTDPTDSGPQPRGKQVTKPGGIWYTPTSGIWQTVWLEPVEKTW
                     IGDLRVVPDIDNGVLHVTVIEEQCKGGPDGCPPVDMAPSLAEITLLAEGETVAVASGT
                     AGTVVTLPVPDARLWRPDDPYLYDLKVRLITGGQVADQVTSYAGMRKISLGTGEDGFT
                     RILLNNEFIWQNGPLDQGFWPDGLYTPPSDEAMVYDLEMLKKMGFNMLRKHVKIENRR
                     FYYHTDRMGLLVWQDMPSGDEYIWGDKPDIVKSAADSAIFMDELTMMIGTKFNHPSIV
                     MWVVYNEGWGQYETPAVTGYVMALDTTRLVNSASGWTDRGTGHVLDIHHYPDPVAPGP
                     EKERAIVLGEFGGLGLPVEGHTWEQKNWGYEKMGDTTSLLNKYSEFYAAVRQMVAEKG
                     LSASVYTQTTDVETETNGLMTYDRKVDKMGYENVRKAMEGSK"
     gene            complement(2264..2815)
                     /locus_tag="PLA17_03505"
     CDS             complement(2264..2815)
                     /locus_tag="PLA17_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPK84510.1"
                     /translation="MTEEIKKLWPELEWINDPELREKTARTWEVALERSVLTVDDLNR
                     IPFTLLCGPDLKVTFMDHKRCVVHVARDAGMKMNEFFRDELPVNMDVLISGAILADVG
                     KMLEYELDASGKAVQGNYGKYLRHPFSGVSLAEECGVPPEVCHIIAAHAHEGDLVKRT
                     TEAYIVHHADFMTFLPFKSRLIV"
     gene            complement(2822..4018)
                     /locus_tag="PLA17_03510"
     CDS             complement(2822..4018)
                     /locus_tag="PLA17_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPK84511.1"
                     /translation="MTRRTIVALPGDGIGAIVLKQAIRVLDAAGFNADYVYGDIGWDF
                     WCREGNPLPRRTIDLIAEHKIALFGAITSKPKDDAAAELSPELQGKGFTYSSPIVGLR
                     QHFGLDICVRPCRSYKGNPLNFIRRDKDGNPEEPMVDVVIFRQNTEGLYGGVEWSDPP
                     QQVYDALMTHHKFRQNFGWCPKEELAVSTRIFTKKYTGRIVRAAFEHAVKFGYKSVTV
                     CEKPNVIRETSGLMWKIAREMQKNEFPGIILRNTNIDAQMMWLTKNPEDYGVIVAGNM
                     FGDIVSDAFAGLIGGLGFACSAQFSEDGIGVFEPTHGSAPKYAGYEVPIVNPVAMIES
                     ACMMLDYIEEKDIATKIRRAIAEVIEEGKYKTYDMMKMSGKPEVVKMGAASTIQMTDA
                     IISKLK"
     gene            4176..5636
                     /locus_tag="PLA17_03515"
     CDS             4176..5636
                     /locus_tag="PLA17_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPK84512.1"
                     /translation="MGELGEMKPRKLWGYFEEICKIPRLSKNEGRIRAYLLDFAKQHN
                     LEAKEDHVGNILIIREAAPGKEKVMTVVLQSHLDMVGEKNASHPHNWAEDPIIPVIDG
                     KWVTASGTTLGADDGIGIAAQMAVLTDANLQCGRIECLFTVDEESGMTGAINLKPDFL
                     TGRILLNLDSEDEGILYIGCAGGIDTQAFLTYTPERVTTGHRAMNISVTGLHGGHSGD
                     EIHKGYGNSIKIITRLLQDLEKKYDIRISNFSGGNLRNAIPREAFVTVTFNPAHEEKI
                     LADTAAYNTLIRDEYNSLEPDLKVAATPADVPELVIDGGTQHRLLGALSLVPHGVLRW
                     SKEIPDLVETSSNLATIRLTDDNMIHVVTTQRSSSEESKHYAAVKAEASFELAGAKVT
                     HSDGYPGWQPNMNSAILRQMIDSYRKLFGKVPQVKAIHAGLECGLILEKYKGIDMISF
                     GPTIRGAHTPEERIEIETVQMFWDLLIDVLRHIPEE"
     gene            complement(5639..5800)
                     /locus_tag="PLA17_03520"
     CDS             complement(5639..5800)
                     /locus_tag="PLA17_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPK84513.1"
                     /translation="MIRYICPVCGYIYDPEQGDPHSGVEPGTPFEDLPSGWVCPVCGV
                     PHDEFEEVS"
     gene            complement(5817..7367)
                     /gene="citF"
                     /locus_tag="PLA17_03525"
     CDS             complement(5817..7367)
                     /gene="citF"
                     /locus_tag="PLA17_03525"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPK84514.1"
                     /translation="MKLVENAAGRLVPDEINGKEAIPFMGVGKYRPSGRKYGPSIPTC
                     IDYPEDNNKVVGSLKEALVRCGLKDGMTVSTHHHLRDGDLVSNQIFAIASELGVKDLV
                     WLPSASFPCNDPVIQYLEDGTVNRIEGSMNGPLGMFVSEGRMKGMAVLRSHGGRVQAI
                     QDGDVKIDIAVLAAPAADSFGNARGTGGPSATGVIGYAKPDYLYAEKVIVVTDNLVNL
                     PCFPMEIEGNYVDYVVVTDKIGIPEKIVSGTTQITKSPDRLLIAELTTSFLEKSGLLR
                     DGATMQAGAGGTSLAIAVYVHQLLKKKGWKLSFGFGGSTKYMVDMLEEGHMGYILDAQ
                     AFDLDSVASIEKNLNHIAYPVFNAYNYHSKGNLTTMMDIMILGATEIDTGFNGNVVTH
                     SDGLLLHGIGGWQNCLHARNVIVPVPLFRDRIPVIVDRVTTLCGPGELIDVIITERGI
                     AINPRRQDLIDAVRGKGLPLVTIEELKDIAEKICGKPEKADFDDRVVAAIKWVDGTVI
                     DAVRKVKR"
     gene            complement(7380..8600)
                     /locus_tag="PLA17_03530"
     CDS             complement(7380..8600)
                     /locus_tag="PLA17_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPK84515.1"
                     /translation="MKQVITGNTGPRIRSDIEVMLELTETGGIELNLKSKVKTMYGKA
                     IERQCRDLLHYFGIENARLSMNDSGALPFVIAARIEAAVKALTGTEKCFIPEMAAENL
                     YASSRDRFRFSRLYLPGNSPGMFLNAGLHSPDGVILDLEDSVAPERKDEARILVRNAL
                     RVVNFYGAERMIRINQGERGLDDLEMLIPHNVHLVLIPKCEDAETVRKVDNRIREIKA
                     REGQNEMVFLMPIIESAAGVEHAMEIATAAKSVVAMAIGLEDYTADLGVQRTKEGKES
                     LYARNRLVVASKAAGIQPIDSVFSDVGDMEGLLNNVLSAKAMGFEGMGCIHPRQIAVI
                     REGFSPSPQELEKAKKIVIAYRDALEKGLGVVALGTKMIDPPVVARAEKTITLAVRLG
                     LLPENWIDLEESKN"
     gene            complement(8760..9188)
                     /locus_tag="PLA17_03535"
     CDS             complement(8760..9188)
                     /locus_tag="PLA17_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPK84516.1"
                     /translation="MKNFKFKINGNQYDVEVLGIDGSMARIDVNGTVYDVEMQRDTPK
                     TKAVVSSASTHARPLKETKEAPESASGKATEIKAPLPGIIIQVLVKVGDDVKPGQTVC
                     TLETMKMENAIKAETAGKVTAVNVSPSQSVLQDEVLVALG"
     gene            complement(9211..9576)
                     /locus_tag="PLA17_03540"
     CDS             complement(9211..9576)
                     /locus_tag="PLA17_03540"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HPK84517.1"
                     /translation="MNWIYLIEGSQILDQLNWIVIVVGIGIILLSLLFVYWFIKYIIK
                     IILNLIPGKSARKKGTEPVVVQTADTTESDEVNAAIAMTIYLYFNELHDVESGVVTIK
                     RISRHYSPWNSKLYHMRNL"
     gene            complement(9589..11148)
                     /locus_tag="PLA17_03545"
     CDS             complement(9589..11148)
                     /locus_tag="PLA17_03545"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPK84518.1"
                     /translation="MKSQERIKQLLDYREKARKGGGEKRIKDQHSKGKLTARERINLL
                     LDEGSFEEFDMFVTHRTEDFGLADKKFLSDGVITGHGTIDGRVVYVFAQDFTVFGGSL
                     SETFAQKICKIMDKAMQAGVPVIGINDSGGARIQEGVRSLAGYAEIFERNILASGVIP
                     QISAIFGPCAGGAVYSPALTDYIIMSRKSSYMFVTGPKVAKAVTGEDITIDELGGAGV
                     HTSKSGVAHFAVDEEQEGLDLIRKLLSFMPQNNLEEPPQLECDDPIDRLEDELNDIIP
                     ESPNQPYDVKDVIVRVVDHHDFLEVHAAYAKNIVVGYARFDGMPVGIVANQPAYLAGV
                     LDIEASRKAARFIRFCDAFNIPIVTFVDVPGFLPGSTQEYGGIIIHGAKLMYAYGEAN
                     VPKVTVTLRKSYGGAHCVMSSKQLKGDINYAWPTAEIAVMGPQGAIEVLEGKAISEIA
                     DDEERKEYVAKKEQEYRDKFANPYEAAKYGYLDDVIEPRNTRFRVIRALRTLSTKKDP
                     GPMKKHGNIPL"
     gene            complement(11162..11581)
                     /gene="mce"
                     /locus_tag="PLA17_03550"
     CDS             complement(11162..11581)
                     /gene="mce"
                     /locus_tag="PLA17_03550"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPK84519.1"
                     /translation="MKPTHIEHIGIAVKSIDEALPFYENVMGLKCYNIEEVADQKVRT
                     AFFMIGQTKIELLESTDPEGPVGKFIEKRGEGIHHIAFAVDQIEDKLRKAEAAGIRLI
                     DSTPRKGAEGLSIAFLHPKSTFGVLTELCEDKNSKNK"
     gene            complement(11674..13236)
                     /locus_tag="PLA17_03555"
     CDS             complement(11674..13236)
                     /locus_tag="PLA17_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPK84520.1"
                     /translation="MKSSALKHIRTVFASLVLLLFVFLFVDFRGVLGDKAFGPILHLQ
                     FVPSFLKFVIAGVAASTGFIIVLVLTLLTGRTYCSFLCPLGILQDVISRAGGLVKRRF
                     RKYGYKKPYTILRYFILVITAALLAIGGVYVLTILDPYSTFGRLMTCFVQPVVLLINN
                     LFAGILGKFGIYSVYHVDIKPYELAAYIVPAAFLLLIGLLAFRYGRLYCNSVCPVGTL
                     LGLLSRVSLFRIRFDEDKCTRCGRCAMVCKSSCIDFLNKSVDLSRCVNCFNCVDACRG
                     NSMSYGLVRLKRQQKDSDEPDSSRRGFIAGSLALLAGGSQLLKAQDTTPSPAKESTVR
                     EDRLYPVSPPGSSGIEMFTSRCTACTLCVNACPTNVLQPSTSQYGFAGIMQPRMDFHS
                     GFCNYDCTRCSEVCPTGAIMPLMPEAKKLTQIGKAVFIKENCIVNTEKTACGACSEHC
                     PTKACHMVPFEGSLLIPEIRDEICIGCGACEYACPTRPFRAVFVDGNPIHEAASRPET
                     EELKEVPTEFPF"
     gene            complement(13243..14169)
                     /locus_tag="PLA17_03560"
     CDS             complement(13243..14169)
                     /locus_tag="PLA17_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPK84521.1"
                     /translation="MKRRSFLRNSLGAGLAAGAFMSFGGYKNAFAGKPLPSGTDYDLI
                     AVMGGEPAAMFDMGIQALGGMGTFVKKGQKVLVKPNIGWDVVPEKGGNTNPALVKRII
                     EHCYRAGAKEVLVFDNTCDNWIKCYKNSGIEQAVRDAGAKMVPSNSESYYQEVTIPNG
                     KKLTKVKVHELVLSSDVFINVPILKHHSSALMTGALKNMMGVVWDRSYWHRNHLNQCI
                     ADYALFSKKPNLNVMDAYNVMMRNGPRGVSVADLSNMKSLMLSTDWVAIDAAGAKLMG
                     REPSSIEHIKLAAGMGIGKMDLAAMNIHRMKM"
     gene            14321..15790
                     /gene="larC"
                     /locus_tag="PLA17_03565"
     CDS             14321..15790
                     /gene="larC"
                     /locus_tag="PLA17_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPK84522.1"
                     /translation="MKILYYDCFSGISGDMNLGALLDLGVSSEILTDGLKKLNTGGWR
                     LDIRRDQRHGITGTKVTVITDHDDYEHPYGNDHKQCHDSQHDHEHSHEQHHHNHLEHE
                     HNNGQRHESHQDHGHDHGHHHDNLHDHGYTHEQCHDSHHDHGHSHEQCHDNHHEHSHE
                     QHQDHHHEHRNLADVEKIINESGLPAPVRELAMKIFRHIAEAEAVVHDKPLHEVHFHE
                     VGAIDSIVDIVGAAICLNEIGADRVYVSEIELGSGMVRCQHGLLPVPAPATTRIISGF
                     PVHIGGVDFEATTPTGAAIIAAIAEPLPPSMRMVIRKSGYGIGQKNNPSRPNILRVCL
                     AESAGEAERGHPARLVECNVDDMNPEMSEYISGRLFAAGAGDVWFTPVIMKKGRPAFT
                     ISVICEEDQLDAVREILFTESTTIGLRVLPFSKETLHREFEEVDTRFGKVTVKKSYFN
                     GRLVSVKPEADRCAAIAATTGIPMKNVISEITSLIRDES"
     gene            15787..16590
                     /gene="larE"
                     /locus_tag="PLA17_03570"
     CDS             15787..16590
                     /gene="larE"
                     /locus_tag="PLA17_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPK84523.1"
                     /translation="MTAARKLDRLRSILSGSGSAVIALSGGTDSTFLASVAAEVPGLR
                     LMAVTVSTPYMFSSEVDEAARFCGEKGIPHRQAAFDIPSSVASNPPDRCYLCKKEVMK
                     AISAVAREEGYASVFDGTNADDLLDYRPGLRALEEEGVRSPLAEAGLTKDEIRALARV
                     AGLDAADRPSDTCLLTRFPHDTEIKHSDLRRAGEAERIVSEAGFAGARVRVHGELIRL
                     ELMKEQFELLALTETRMTITSALKKLGYRYITIDAEGYRSGSMNKTTKK"
     gene            16587..>16807
                     /locus_tag="PLA17_03575"
     CDS             16587..>16807
                     /locus_tag="PLA17_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84524.1"
                     /translation="MNSSELEKILGEIRAGKVSVEEAMEKLRDFPYTDLGFARIDNHR
                     ELRRVPRDSLLCRQGPGAGQGYFQGHGGT"
BASE COUNT         4113 a         4281 c         4275 g         4138 t
ORIGIN      
        1 cagcatgccc tgaagctcta ccttgaaagc cacaacatgt cggccaccac cgaggaaggc
       61 aagcgcgccg ttctcactct catagagaac gaagtgaatg aataccgcat taacggcaag
      121 tacggccaca tgttcccgca ccgctggctg ccggtagcgc tgggagtact caatgagagc
      181 ttcaatgaag agaacggcat gatgaactca accatgaaga ttgtcagggg aaagataacc
      241 gaccgttatc atgacctcat cgagtggctc tacaccccta tgggcaagat agtacacaat
      301 gaacgcaaca tggaagaagt tgagaagatg aaactcggct gaccagttaa aacccttcac
      361 agatgaaata ctttttaatt cctgttcttg ttgtaatttc cgggattatg acctgttgcc
      421 acagggagaa agccccggag tggagacctg ctgaaaaccc gatttataca gtctggggtg
      481 agaaacttga tccttcggca ccctggccgg aatacccgag accggcactt gccagggatg
      541 aatggatgaa cctgaacggt ttatgggact atgccatctg tccgaaagat gcagatcgtc
      601 ctgaaccaca gggaaaaatc ctcgtaccct tccctgttga atcagctctc tccggtgtca
      661 gggaaagggt gaatgactcc ctggcgctgt ggtattcacg ggatttcgtt ctgccgaaac
      721 aatggaagga taagcggatc ataatcaatt tcgaagcttc cgactgggag actaccgtat
      781 gggttaatga caagctggca acagtacacc ggggcggtta tgatcccttc tggtgtgaca
      841 tcaccaattt gcttgatggc agaaagaagc atacgcttgt tgtcagggta actgatccca
      901 ccgacagtgg cccccagcca aggggcaagc aggtaacgaa gcccggtggc atctggtaca
      961 ctccaaccag cggcatctgg caaaccgtat ggcttgaacc ggttgaaaag acctggatag
     1021 gggatcttcg tgtagtgcct gacattgaca acggggtgct tcacgtgacg gtcatcgagg
     1081 agcagtgcaa gggaggtccg gatgggtgcc cgcccgttga catggccccg tcacttgcgg
     1141 agataaccct gcttgctgaa ggtgaaacgg tggctgtggc atcaggtaca gcaggcaccg
     1201 tggtgacact gccggtgcct gatgcaaggc tctggcgccc tgatgacccg tacctctatg
     1261 acctgaaggt gagactgata accggaggcc aggttgctga ccaggtgacc tcctacgccg
     1321 gtatgagaaa gatatcactg ggaacaggtg aggatggctt tacccgcatt ttactgaaca
     1381 atgaatttat ctggcagaac ggtccgcttg accagggatt ctggcccgac ggactttata
     1441 ccccgccatc cgatgaagca atggtttatg accttgagat gcttaagaag atggggttca
     1501 acatgctgcg caagcatgtc aagatagaga acaggaggtt ctattaccac actgaccgga
     1561 tgggcctgct ggtgtggcag gatatgccca gcggcgatga atacatctgg ggtgacaagc
     1621 ctgacatagt caagagtgca gctgactctg ccatcttcat ggatgagctc acaatgatga
     1681 tcggcacgaa gttcaatcac ccgtcaattg ttatgtgggt tgtttacaac gaaggctggg
     1741 gccagtatga gacaccggca gtaaccggtt atgtcatggc tcttgacacc acgaggctgg
     1801 tcaacagcgc ctcggggtgg actgacaggg gtacggggca tgtgcttgat atacaccact
     1861 atcctgaccc tgtggctccc ggtcctgaaa aagagcgggc cattgtgctg ggtgagttcg
     1921 gagggctggg tctgccggtt gagggacata cctgggaaca gaagaactgg ggttatgaaa
     1981 agatgggaga caccacctcc ctgctgaaca aatacagtga gttctacgct gccgtcaggc
     2041 agatggttgc cgaaaaggga ctgagtgctt cagtctacac acagaccact gacgtggaaa
     2101 cggagaccaa cggcctgatg acatatgacc gcaaagtgga caagatgggg tatgaaaatg
     2161 tgagaaaggc gatggaaggc agtaaataaa acagtagtca gcatctgagc aggtaattga
     2221 tttgctgcag attactgact acagaatgat aagggcaggc tgatcagacg atcagcctgc
     2281 ttttaaatgg cagaaatgtc atgaagtctg cgtgatgcac aatataggct tcggtggtac
     2341 gctttaccag gtcaccctcg tgggcatgtg ctgcaatgat atggcacact tcgggtggca
     2401 caccgcactc ctctgccagg ctgacgcctg agaaggggtg tctcagatac ttcccgtagt
     2461 tcccctgcac agccttcccg gatgcatcga gttcatattc cagcatcttt ccgacatcag
     2521 ccaggatagc gcctgagata agcacatcca tgttgaccgg cagttcatcg cggaagaatt
     2581 cgttcatctt catccccgca tcgcgggcaa catgcaccac gcatctcttg tggtccatga
     2641 aggtgacttt caggtcagga ccgcaaagga gggtgaatgg tatgcggttc aggtcatcaa
     2701 cggtaagcac gctccgttcg agggcaactt cccacgtgcg ggcggtcttc tccctcagtt
     2761 caggatcatt gatccattcc agttcaggcc acagtttctt tatctcttcg gtcatatcct
     2821 gttattttaa ttttgaaatg attgcatctg tcatctggat ggttgaggct gcccccatct
     2881 ttaccacctc gggcttgccg ctcatcttca tcatgtcata ggtcttgtac tttccctctt
     2941 cgatgacttc cgcgatagcc ctgcgtatct ttgtggcaat atccttttcc tctatgtagt
     3001 caagcatcat acatgctgac tcgatcatag ccacagggtt aactataggt acttcatagc
     3061 cggcgtattt gggagctgat ccgtgagtag gttcaaacac cccaatccca tcttctgaga
     3121 actgagcgct gcatgcaaat ccgagtccgc cgatcagccc ggcaaaggcg tcggaaacga
     3181 tatctccgaa catattaccg gcaactatta cgccataatc ctcgggattc tttgtgagcc
     3241 acatcatctg tgcatctata tttgtgttcc ggagaatgat gcccgggaac tcattcttct
     3301 gcatctccct ggctatcttc cacatgagac ctgaggtttc acgtatcacg tttggttttt
     3361 cacagacagt gactgatttg tagccgaatt taacggcatg ttcaaatgct gccctgacaa
     3421 tgcgcccggt atatttcttc gtgaagattc ttgttgagac tgccagttcc tctttcgggc
     3481 accatccgaa gttctggcgg aacttgtgat gggtcatcag cgcatcatag acctgctgcg
     3541 gaggatcgga ccattccaca ccgccataga gcccttcggt attctgcctg aatatcacta
     3601 catctaccat cggttcctca ggatttccgt ccttatcgcg gcggatgaag ttgagggggt
     3661 tgcccttgta gctgcggcac ggtctgacgc agatatctag gccgaagtgt tgcctgaggc
     3721 ccacgatggg gctgctgtaa gtaaagcctt tgccctgaag ttccggtgag agctcggcag
     3781 cggcgtcatc ctttggtttg gaggtgatgg caccgaagag cgcgatcttg tgttcagcaa
     3841 tcagatcaat ggtacgccgg ggaagaggat taccctcacg gcaccagaaa tcccagccga
     3901 tatctccgta aacataatca gcattaaaac ctgccgcatc aaggacgcgg attgcctgct
     3961 tcaaaacaat ggcacctata ccgtcacccg gcagtgccac aatagtacgt ctggtcataa
     4021 ttttattctt taaactgaat tatttcgttt ccggtaaaaa tatatttttt cctgacttaa
     4081 aaaaaagagt ttcagcaggt aaaattcata gaggttgcaa cggaaaaaaa ttatatttgg
     4141 ggctaaatca tttaacataa aagacaagac acagaatggg tgagttgggg gaaatgaagc
     4201 ccagaaaact ctggggttac tttgaagaaa tctgcaagat accacgactg tcgaagaacg
     4261 aaggaagaat cagggcatac ctgcttgatt tcgcgaagca gcacaacctt gaggcaaagg
     4321 aggaccatgt agggaacata ctgattatca gggaagctgc ccctggtaag gagaaggtta
     4381 tgacagtggt tcttcagagc cacctcgata tggtaggtga aaagaatgcc tcccatccgc
     4441 ataactgggc cgaggatcct attataccgg ttattgacgg aaagtgggta accgccagcg
     4501 gcacaacact tggcgctgat gatggtatag gcattgcagc acagatggct gtgctgacgg
     4561 atgcaaacct tcaatgcgga cggatcgagt gcctctttac cgttgatgag gaatcaggaa
     4621 tgaccggagc aatcaacctc aagcctgatt ttttaacagg acgcatatta ttgaacctcg
     4681 actcggagga tgagggtatt ctctacatcg gctgtgccgg cggaattgat acccaggcct
     4741 tccttaccta cactcctgag agggttacta cagggcacag ggccatgaac atctccgtca
     4801 ccgggctgca cggcggccac tcaggcgatg agatacacaa gggctacggt aattccataa
     4861 agataataac caggctgttg caggatcttg agaagaagta tgatatcagg atcagcaatt
     4921 tctccggcgg caacctccgc aatgcaatac cgagggaggc gtttgtcacg gtgacattca
     4981 atccggcaca tgaagaaaag atactggcag acacggcagc ctacaatacc cttatcaggg
     5041 atgagtacaa cagcctggaa cctgacctga aggtagcagc aaccccggca gatgttcctg
     5101 agctggtcat tgacggcgga acacagcaca ggttgctggg tgcactgagc ttagtgcctc
     5161 acggtgtgct ccgctggtca aaggagatac ctgacctggt tgagacatca agcaacctgg
     5221 caacgataag gctcaccgat gacaacatga tccacgttgt caccactcag aggagttctt
     5281 cagaagagtc aaagcactat gccgcggtga aggctgaggc aagcttcgag ctcgcagggg
     5341 cgaaggtgac ccattctgac ggttaccccg gctggcagcc gaacatgaac tcggcaattc
     5401 tgaggcagat gattgattca taccggaagc ttttcggcaa ggtgccgcag gtcaaagcca
     5461 ttcatgcagg acttgagtgc gggctgatcc ttgagaagta caaggggata gacatgatct
     5521 cattcggccc taccatcagg ggtgcacata ctcccgaaga gcggatagag attgagactg
     5581 ttcagatgtt ctgggatctg cttatcgatg tactgaggca catccccgaa gagtagtgtc
     5641 agctgacctc ctcaaattca tcatggggca caccacaaac ggggcatacc catcctgacg
     5701 gaagatcttc gaagggtgtt cccggttcca cgccactgtg agggtcaccc tgttccgggt
     5761 catatatgta gccgcatacc gggcatatgt atctgatcat aattactgtt ttactattat
     5821 ctctttactt ttctgacagc atcaataacc gttccgtcaa cccatttgat ggcagccacc
     5881 accctgtcgt caaaatccgc tttctccggc ttgccgcata tcttctcggc aatatccttc
     5941 aactcttcta ttgtcaccag tggcaggccc ttgccccgta ctgcatcaat caggtcctgc
     6001 ctgcgggggt tgatggcaat gcccctctcg gtaatgatca catcaatcag ttcgccaggc
     6061 ccgcagaggg tggtaacccg gtctacaatc accgggattc tgtcacggaa aagaggtacg
     6121 ggaactatga cgttgcgcgc atgcaggcag ttctgccacc caccgattcc atgcagcaga
     6181 aggccatcag agtgggtcac cacgttcccg ttaaagccgg tgtcaatctc ggttgcccca
     6241 aggatcatga tgtccatcat ggtagtgaga ttgcccttcg agtggtagtt gtacgcgttg
     6301 aataccgggt aggcaatatg gttcaggttt ttttcgattg aggcgactga atcaaggtca
     6361 aaagcctggg catcgagaat atatcccata tggccttcct caagcatgtc aaccatatat
     6421 ttggtactgc caccgaagcc aaaactgagt ttccatccct ttttcttcag gagctggtgc
     6481 acgtaaaccg caattgcaag actggttcct cctgccccgg cctgcatggt tgccccgtcg
     6541 cggagcaggc ctgacttctc aaggaaagat gttgtcagtt ctgcaatcag cagcctgtca
     6601 gggctctttg tgatctgtgt ggttcccgag acgatttttt caggaatacc tattttgtct
     6661 gtaaccacaa cataatcaac ataattgccc tctatctcca tcgggaagca tggcaggtta
     6721 acgaggttat cggtcaccac tatcaccttt tcagcataga ggtaatcagg cttggcgtat
     6781 cctatgacgc ctgttgcgga tggtcctcct gttcccctgg cattgccgaa ggagtcagca
     6841 gcgggggctg ccagcacagc gatatctatc ttcacgtcgc cgtcctgtat tgcctgtaca
     6901 cgtcctccgt gcgagcgcag cacagccatc cccttcattc ttccttctga cacgaacatg
     6961 cccagcgggc cgttcatgct tccctcgatg cggttgacag tgccgtcttc cagatactgg
     7021 atgacagggt cattgcaggg gaatgatgct gacggcagcc agacaaggtc cttcactccc
     7081 aactcagagg cgatggcgaa tatctggttg cttaccaggt caccgtcgcg cagatggtgg
     7141 tgagtagata cggtcatacc atctttcagc ccgcagcgta cgagggcttc cttaagggag
     7201 cccacaacct tgttgttatc ctccgggtaa tcaatacatg ttggtatcga aggtccgtat
     7261 ttcctgcctg aggggcgata tttgcccaca cccatgaaag ggatggcttc cttaccgttt
     7321 atttcatccg gcaccagacg tccggcagcg ttttctacaa gtttcatgac ggtaatgcat
     7381 taattttttg actcttccag atctatccag ttctccggca gcagacccag cctgacggcc
     7441 agggtaattg tcttttcggc ccttgccaca actggcgggt ctatcatttt tgtgccgagt
     7501 gcaacaactc caagtccctt ttcaagcgca tcgcggtatg cgatgactat cttctttgcc
     7561 ttttccagct cctgcggtga gggggagaat ccctccctga taacggctat ctggcgcggg
     7621 tgaatgcagc ccattccctc gaagcccatg gcttttgccg agagtacgtt attgagcagc
     7681 ccttccatat cacctacgtc agagaagacc gaatcaatcg gctgaatacc ggctgccttg
     7741 gaagcaacca caaggcggtt gcgggcatag agtgactcct ttccttcctt tgtgcgctgc
     7801 actccgaggt cagcggtgta atcttccaga cctattgcca tggccactac tgatttggca
     7861 gcggtggcaa tttccatggc atgctccact ccggcagcac tctcaatgat aggcatcagg
     7921 aagaccattt cattctgtcc ttcccttgcc tttatctccc tgattctgtt atcaacctta
     7981 cgcacggttt cagcatcttc gcacttcgga ataagcacca ggtgaacgtt atggggtatc
     8041 aacatttcaa ggtcatcaag tccgcgttca ccctggttta tgcgtatcat cctctcagcc
     8101 ccgtagaaat tgacaaccct gagggcattt ctgacaagga ttcttgcctc atcctttcgt
     8161 tccggggcaa ctgaatcttc caggtcaaga atgactccgt caggtgaatg aagtccggcg
     8221 ttgaggaaca ttcccgggct gtttccgggg aggtagagcc ttgagaacct gaacctgtct
     8281 cttgatgagg cataaaggtt ttcggcggcc atctccggta tgaagcactt ctcagtacct
     8341 gtaagggctt ttaccgctgc ttcaatcctg gcggcaatga caaacggcaa cgctcctgaa
     8401 tcattcattg agagcctggc attttcaatg ccgaagtaat ggagaaggtc acggcactgc
     8461 cgttctatgg ccttgccgta catagttttt accttcgatt tgagattcag ctcgatacca
     8521 cctgtttcag tgagttcaag cattacttca atgtcagacc gtattctggg tccggtatta
     8581 cctgttatta cctgtttcat tttctactct ctgaaatgaa attcaaaaac ataataaatt
     8641 atccgggatg gctgaaccgt ataccttctg caattgcagg gccgataaca ccagccacgt
     8701 tcggtcccat ggcgggcacg ggagttgaat ggctttgtca gctttctccg gtttgaattc
     8761 tagcctaatg ccaccagcac ttcatcctga agaactgact ggcttgggga gacattaacg
     8821 gctgtcacct ttcctgccgt ctcagcctta atggcatttt ccatcttcat tgtctcgagg
     8881 gtgcaaactg tctgacccgg tttgacatca tcacccacct tgacaaggac ctgaataata
     8941 attcccggca gcggggcctt gatctccgtt gcctttcctg aagcagattc aggcgcttcc
     9001 tttgtctcct ttaacggcct tgcatgtgtt gaagctgatg atacaacggc tttagtcttg
     9061 ggtgtatcgc gttgcatctc aacatcatag acagttccgt tcacatcgat ccttgccatg
     9121 cttccatcga tcccaagaac ctcaacatca tactggtttc cgtttatctt gaacttgaag
     9181 tttttcatta cacttacgga ttattttttc ttaaaggttt ctcatatggt acaatttgga
     9241 gttccagggc gaatagtgcc tggagatgcg cttgatggtc accaccccgc tctccacgtc
     9301 atgcagttca ttgaaataga ggtaaatggt cattgcgatg gctgcgttga cctcatctga
     9361 ctctgttgta tctgctgtct gaacaactac cggttcagta ccctttttgc gggcagattt
     9421 tccgggtatt aagttcagga taattttgat aatgtacttt atgaaccaat atacgaacag
     9481 cagggagaga agtattattc cgatgcccac cacaattacg atccagttga gctgatcaag
     9541 gatctgtgag ccctcaatga gatatatcca gttcatgatc atttcttatc agagtgggat
     9601 gttgccatgt ttcttcattg gtcccgggtc cttctttgtg gagagtgtcc tgagagccct
     9661 tataaccctg aacctggtgt tccggggttc aatcacatca tcgaggtacc cgtatttggc
     9721 tgcctcgtag gggttggcaa acttgtcgcg atactcctgt tcctttttgg ccacgtactc
     9781 cttgcgttct tcatcatcag caatctcgct gatagctttt ccttcaagaa cctcgatggc
     9841 tccctgggga cccatcactg ctatctcagc agtaggccag gcgtagttga tatcgccttt
     9901 cagctgtttg gatgacatga cgcaatgggc tcctccatat gatttcctga gggtgacggt
     9961 caccttcggc acattggctt cgccgtaggc gtacatcagc ttggctccat gaatgataat
    10021 accaccgtac tcctgggttg agcctggaag gaatcccggg acgtcaacga aggtaacgat
    10081 ggggatattg aatgcatcac agaatcttat gaagcgtgct gccttgcgcg aagcttcaat
    10141 gtcaaggaca cctgccaggt atgcgggctg gtttgcaact atgccaacgg gcataccgtc
    10201 aaaccttgca taaccgacca ctatgttctt ggcataggcc gcatggacct caaggaagtc
    10261 atgatggtca acgaccctta caatcacatc ctttacatca taaggctggt tggggctctc
    10321 cggtatgata tcattcagtt catcctccag tctgtcaatc gggtcatcac attccagctg
    10381 cgggggttcc tcaagattgt tctgaggcat gaagctcagc agtttcctga taagatcgag
    10441 cccctcctgc tcctcatcaa cggcgaagtg tgccactccg gacttggaag tatgaacgcc
    10501 ggcgccgcca agttcatcaa ttgtaatatc ctcaccggta acggcttttg ccacctttgg
    10561 accggtgacg aacatatagc tgctcttgcg gctcatgatg atatagtcag tcagtgcagg
    10621 ggaatagact gcaccgccgg cgcaggggcc gaagatggcc gatatctgcg gaatcactcc
    10681 tgatgcaagg atattgcgct caaatatttc tgcatagcct gcaaggcttc tgacaccttc
    10741 ctggatacgg gcgccgccgg aatcattgat accgatgaca ggaacgcctg cctgcattgc
    10801 cttgtccata atcttgcata tcttctgggc gaatgtttct gacagtgacc cgccaaagac
    10861 cgtgaaatcc tgggcaaaga catagactac ccttccgtcg atggtgccgt ggcctgtgat
    10921 aacaccgtca gagaggaatt tcttgtctgc cagcccgaag tcctcggtcc tgtgggtaac
    10981 gaacatgtca aactcctcga agctgccttc atcaagcagc aggttaatgc gttccctggc
    11041 agtaagcttg cctttggagt gctgatcctt aattcgtttt tcgccgccgc ccttacgcgc
    11101 tttctccctg taatcaagga gttgcttgat tctttcctga ctcttcattg aacctggtta
    11161 tttatttgtt tttgctgttt ttgtcctcgc atagttcggt aagcacaccg aaggttgatt
    11221 tgggatgcag gaaagcgatg ctcagcccct cggcaccttt tctgggagta ctgtcaataa
    11281 gcctgatccc agctgcctca gccttacgga gtttatcctc aatctggtct actgcaaaag
    11341 caatatgatg aattccttct cccctctttt cgatgaattt gcctaccggc ccttccgggt
    11401 cagttgactc gagcagctca atcttagtct gcccgatcat aaaaaaagcg gtcctgactt
    11461 tctggtcagc cacctcctca atgttgtagc acttcaggcc catgacattc tcatagaacg
    11521 gaagtgcctc gtcaatggat ttgactgcaa ttccaatgtg ctcaatatgt gtgggtttca
    11581 taataaatca attaagattg aaataataat aagaaaatgc aagttaaatc tatttaactc
    11641 atccccacac gataaaaatc atgttttcag gtctcagaat gggaattccg tcggcacttc
    11701 cttcagttct tcggtctcgg gcctggaagc agcttcatgg atagggttac cgtcaacgaa
    11761 gacagccctg aagggtcttg tggggcatgc atactcacag gctccgcaac cgatgcaaat
    11821 ctcgtcccta atttcaggta tgagcaggct tccttcaaag ggtaccatat ggcatgcctt
    11881 ggtgggacaa tgttcagagc aggctccgca tgctgtcttt tcagtattga caatacagtt
    11941 ttcctttatg aaaacagctt tgcctatctg agtcagtttt ttggcttccg gcatcagtgg
    12001 catgattgct cctgtggggc agacttcaga acaacgggta caatcatagt tgcagaaacc
    12061 gctgtgaaaa tccatcctcg gctgcattat accggcaaaa ccgtactggc tggttgaagg
    12121 ctggagcaca tttgtggggc aggcattgac gcacagtgta catgccgtgc atctggaggt
    12181 gaacatttca atgccggatg atccgggagg cgataccggg tagagccggt cttccctgac
    12241 tgttgactcc tttgcaggtg atggcgtggt gtcctgcgcc ttcagcaact gcgaacctcc
    12301 tgccagcagg gcgagtgatc cggcaatgaa gccgcggcgc gacgagtcag gttcatcact
    12361 atccttctgt tgccgcttca gtcttaccag accgtatgac atgctgtttc ccctgcatgc
    12421 gtcaacacag ttgaaacagt tgacacagcg gctcagatca actgatttgt tcaggaaatc
    12481 tatgcatgat gatttgcata ccattgcaca cctgccgcat cgggtacact tatcctcatc
    12541 gaacctgatt ctgaagagcg acaccctgct cagcaggcca agcagtgttc caacagggca
    12601 gacgctgttg cagtagagcc tgccgtacct gaaggcaagc agcccgatca gcagcaggaa
    12661 tgctgctggc actatgtagg cggcgagctc atagggtttg atatccacgt ggtataccga
    12721 atagatgccg aatttaccca ggatgcctgc gaacagattg ttgattaaaa gcaccaccgg
    12781 ctgcacaaag caggtcatca gcctcccaaa ggtactgtac ggatcaagga ttgtgagcac
    12841 atagacgcct ccgatagcca ggagtgccgc tgttatcaca aggatgaagt aacgcagtat
    12901 ggtgtagggt tttttgtaac cgtacttgcg gaaccgtctc tttaccagcc ctcctgcccg
    12961 gcttatcaca tcctgaagta tgcccagcgg gcagagaaat gaacagtaag ttctcccggt
    13021 gaggagggtc agaaccagaa cgattatgaa tccggttgag gctgccacgc ctgcgatgac
    13081 aaacttgagg aatgagggca caaactgaag atgaaggatc gggccgaaag ccttatcgcc
    13141 aaggactccc ctgaagtcta cgaacaggaa tacaaacagc agaagcacga ggctggcaaa
    13201 tacggtacgg atgtgtttca gggcggagga cttcatgagt ggttacattt tcatcctgtg
    13261 tatattcatt gctgcaaggt ccatttttcc tattcccatt cctgcggcaa gttttatatg
    13321 ttctatggat gagggttccc ttcccatgag ttttgcgcct gctgcatcaa tggccaccca
    13381 gtcagtagag agcatgagtg atttcatatt tgagaggtca gccactgaca cgccacgtgg
    13441 cccgttacgc atcatcacat tgtaggcatc catgacattg aggtttggct tcttcgaaaa
    13501 cagtgcgtag tcagcaatgc actggttgag atgattcctg tgccagtagg aacggtccca
    13561 taccaccccc atcatgttct tgagggctcc cgtcatcaat gccgaactgt ggtgcttgag
    13621 gataggaaca ttgataaaga catcgctcga aagaacaagt tcatgaactt tgaccttcgt
    13681 cagttttttc ccgttgggaa tggtcacctc ctgataatag ctttccgagt tgctcggcac
    13741 catctttgct ccggcatccc tgacagcctg ctcaatgccg gagttcttgt agcatttgat
    13801 ccagttatcg catgtattgt caaataccaa tacctccttc gctcctgccc tgtaacaatg
    13861 ctcaataatc ctcttcacga gggcagggtt ggtattccct cccttttcag gcaccacgtc
    13921 ccagccgata ttcggcttca ccagcacctt ctgtcctttt ttgacaaaag tacccatgcc
    13981 gcccagtgcc tgtatgccca tgtcaaacat tgcagccggc tctcccccca tcactgcaat
    14041 aaggtcataa tcagtacctg aaggaagagg ctttccggca aatgcattct tataaccacc
    14101 aaaactcatg aaggctcctg cagccagacc ggcaccaagt gaatttcgca agaaacttct
    14161 ccttttcatt accttccgtt tgatattacc ccaaaaatga gaaatgcaaa gtaaacattt
    14221 tttttaatat atcggctccg tattcttata ttagcagcct gagaatgcga ccttaagcga
    14281 ttatgcgctg ccggcaggtg atgttaaaaa aactaaaaaa atgaagatac tatattacga
    14341 ctgcttttca ggaatcagcg gagacatgaa cctgggtgca ctgcttgacc tgggagtaag
    14401 cagtgagata ctgaccgacg gcctgaagaa actcaataca ggtggctgga ggctggatat
    14461 caggcgggat cagagacatg gcatcaccgg cacaaaagtt acagtcatca cagatcatga
    14521 tgattatgag cacccgtacg ggaatgatca caagcaatgc catgacagcc aacatgacca
    14581 cgaacacagc catgagcagc accaccacaa ccacctggag cacgagcaca ataatgggca
    14641 gcgccatgag agtcaccagg accatggaca cgatcatggg catcaccatg acaatcttca
    14701 tgaccacgga tacactcacg agcagtgcca tgacagccac catgaccacg ggcacagtca
    14761 tgaacagtgc catgacaacc accacgagca tagtcatgag cagcaccagg accatcacca
    14821 tgagcacagg aatcttgccg acgtggaaaa gataatcaat gaaagcggtc tgccggctcc
    14881 ggtcagagag ctggcgatga agatattcag gcatatagcc gaagctgaag cagttgtaca
    14941 tgacaaaccc ctgcatgagg ttcattttca tgaggtgggt gcaattgact ccattgttga
    15001 catagttggt gcagccattt gcctcaatga gatcggcgct gacagggttt atgtttcgga
    15061 aattgaactc ggaagcggta tggtcaggtg ccagcacggg ctgctgcctg tccctgcacc
    15121 tgcaacaaca cggatcatca gcggttttcc ggttcatatc ggcggggttg attttgaagc
    15181 caccaccccc accggggctg caatcattgc tgccattgca gaaccattgc cgccatcaat
    15241 gagaatggtc atcaggaaga gcggttacgg cattggtcag aaaaacaacc cttccagacc
    15301 caacatactc agggtctgtc tggcggagag tgccggtgaa gctgagagag ggcatccggc
    15361 aaggcttgtt gaatgcaatg tggatgacat gaatcctgag atgtcggaat acatatcagg
    15421 caggcttttc gccgcaggcg caggagacgt atggttcacg ccggtgataa tgaaaaaggg
    15481 gcggccggcc tttaccatca gcgtcatctg cgaggaggat cagcttgatg ctgtcaggga
    15541 gatactgttc accgagtcaa ccacaatagg gctgagggta ctgccctttt ccaaggagac
    15601 acttcaccgc gaatttgaag aggttgatac caggtttggg aaggtgacag tcaagaaatc
    15661 atatttcaac ggaaggctgg tgtcggtcaa gcctgaagcg gacaggtgtg cagccattgc
    15721 agccacgacg ggcataccga tgaaaaacgt gatcagtgag ataacctctc taatcaggga
    15781 cgaatcatga ccgctgcacg gaagcttgac agactcaggt ctatcctttc cggatcaggt
    15841 tcggcagtaa tagccctgtc gggtggtact gacagcacat tcctggccag tgttgccgca
    15901 gaggtgcccg gactccgcct gatggccgtg acggtaagca ccccgtacat gttctcatca
    15961 gaggttgatg aggctgcccg cttctgcggg gagaagggca ttccccaccg gcaggccgct
    16021 tttgacatcc cgtcttcggt tgcctcaaac cctcccgacc ggtgttatct gtgcaagaag
    16081 gaggtgatga aagccatctc cgccgtggcg agggaagagg gatatgcatc agtcttcgat
    16141 ggcaccaacg cagatgacct gcttgattac cgtccgggac tgagggctct tgaggaagag
    16201 ggagtaagaa gtccgcttgc tgaagccggt ctgacaaagg atgagataag ggcgctggcc
    16261 agggtggccg gtctggatgc agctgacaga ccctctgaca cctgcctgtt gacccgcttc
    16321 ccacatgaca cggagataaa gcactctgat ctcaggagag ccggggaggc tgaaaggata
    16381 gtgagcgaag caggattcgc gggcgcccgg gtaagggtgc acggtgaact gatcaggctt
    16441 gagctgatga aggagcagtt tgaactgctt gccctgactg agacaaggat gacaattacc
    16501 tctgcactga aaaaacttgg ttacagatat attacaattg acgctgaagg ttacaggagc
    16561 ggaagcatga acaaaacgac gaaaaaatga attcatcaga gcttgaaaag atacttgggg
    16621 agatcagggc cgggaaggtg agtgtagaag aggctatgga gaagctgcgt gactttccct
    16681 atacagacct gggatttgcg cgcatagata atcaccggga gctgaggagg gtaccccgag
    16741 atagtttact gtgccggcaa ggccccggag caggtcaggg ctattttcaa ggccatggag
    16801 ggacaca
//
LOCUS       DAOPBK010000037        16627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3531_length_16627_cov_5.111151, whole genome shotgun sequence.
ACCESSION   DAOPBK010000037 DAOPBK010000000
VERSION     DAOPBK010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3531_length_16627_cov_5.111151"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLA17_03580"
     CDS             complement(<1..321)
                     /locus_tag="PLA17_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HPK84525.1"
                     /translation="MNIDLNKKLPAYLTEKGNIVTTILFTAGFALLFLNLYTPFGVST
                     WFEVSKPTLLFYSSGVILAGVLVVAISRILMYHHVKRGKSLLIWHYLIWVAAEIIAMA
                     LFFTL"
     gene            709..1875
                     /locus_tag="PLA17_03585"
     CDS             709..1875
                     /locus_tag="PLA17_03585"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HPK84526.1"
                     /translation="MGYTATERQISSHFREGLAEGVIPGTGVTVSVRRFSSAVDSSLR
                     DGQNVPGLFRSLFRSREVEESNREFSYYILSPCVDSSKPCDGSGAIILLHGLNERNWS
                     KYIQWGVSLADRTGRPVVLFPLAYHMNRSPRSWIDRHIMMPLVSARQALMPDARLSTF
                     VNVALSTRMTASPQRFMLSGYQTARDLTALIRSMREGSIPGIAAGGRTDFFAYSIGAL
                     LIQVMMLSDTALLPDDSRIMLFCGGSAFSMMNGTSKLIMDSRAFEMLLSFYIGWPESI
                     MNGGRKRLTRLMNDTPEGRAFFAMTTLQRLENLRGRPFNRYDGRLKAVTFESDTVIPA
                     KAVMETLSGADTEVWDPGYPCTHESPFPVLAGANEIKVDRTFDRLFDTAARFFA"
     gene            1915..2826
                     /locus_tag="PLA17_03590"
     CDS             1915..2826
                     /locus_tag="PLA17_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPK84527.1"
                     /translation="MKSLLIPPYLREGDRIEIISPASHIEEGVVHSAVQMLEESGFRV
                     TLGSHVFTRSGPFAGNEAERLHDIQQATDDPDVKAVICSRGGYGMSRIVDRIDFSALK
                     KQPKWYAGYSDITSLHMWLNRVCGIASLHAEMPLNYSNSEYLPVCYDTLVKALRGEAE
                     PVRWLPLHEASLEVSGHVTGGNISLIYSLNGTPAQPDTEGAILFIEEIGERFYHLDRM
                     LVAMRMAGVLRNLSALVVGGMEKITEGEHVFNQTIEEVVMNVVAGYDYPVLFNFPAGH
                     IPDNRAIYLGRNARITQSGSEAVMSWI"
     gene            complement(2985..4181)
                     /locus_tag="PLA17_03595"
     CDS             complement(2985..4181)
                     /locus_tag="PLA17_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPK84528.1"
                     /translation="MNEPARSALLPREEQIEKAVGRKQMSIGLPRDVHDDEKRIPLTP
                     EAIRILTDAGNEIFMERGAGQAAGFTDREYSDNGAIVADTPSRIYECDIIIKITPFKQ
                     NEASQLKVGQTVISFMNVATMDEDVLSTLMRKRVTALASEKIKDTDGMLPVVESMSEI
                     SGITSVLLASEYLSSASGGKGVMLGGVTGVTPTEVIIIGANTAGEYAARAALGLGAIV
                     RVFDASVHRLRNFQNLLGQRIYTSTFHPQVLQKALKSADVLIGALSSDILMPWFYVTE
                     EMVQGMKRGSVIIDLSVDTGGCVETAEPRAIKDPIYEKHGVIHYSAWNLPSRVPKTAS
                     IALSNVFRPLMQEMADAGSITHMLRFNPGLRSGVYIYNGILTNDVLGQKFGILSQDIN
                     LLLAAF"
     assembly_gap    4317..4416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4417..4765)
                     /gene="tsaE"
                     /locus_tag="PLA17_03600"
     CDS             complement(<4417..4765)
                     /gene="tsaE"
                     /locus_tag="PLA17_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPK84529.1"
                     /translation="MKIVIADKSRLHDAARHFLKETAGKRLFAFYGEMGSGKTTFIRA
                     LCHEMDVADTVTSPTFTLVNEYRRPGSTPVYHFDFFRIKKITEVLDFGIEEYFDSAAP
                     CFMEWPELIEPLLP"
     gene            complement(4783..6336)
                     /locus_tag="PLA17_03605"
     CDS             complement(4783..6336)
                     /locus_tag="PLA17_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HPK84530.1"
                     /translation="MRQIRILWVDDEIGVLRSYILYLEEKGFETDTCHSGNDAIAMVR
                     EKAYDIIFLDEHMPGLSGIETLRRIKAIRPAIPVVMITKSEEEDIMDAAIGSQIADYL
                     IKPVKPQQILLTLKRILNIEEIVARQTTTVFREEFSSIADMISSASTFEQWSELYRRL
                     TFWQGELIRSPDTGMREVLRMQESDANRAFAKYISGNYMKWVSAGAADRPLMSPAVLS
                     KRIFPMISRETPLFFIIIDNMRLDQWQTIAGDLRKVMRIVDEELYLSILPTTTQYSRN
                     ALFAGLMPAAIEELMPEYWIDDHEDEGKNLHEEQLLSKNLLRAGINCRWSYNKINSDA
                     EGRSVNDRIAQLMGNDLNVLVYNFVDMLSHARTEINLIRDLTADEASWLTLTHSWFIH
                     SPLMELLRLVSSRGARVVFTTDHGSVRVQRPLKVVGDRQTSMNLRYKMGRNLDYNPRE
                     VFEITAPAKAGLPMVNITSRYIFACANDFLVYPNNYNSVVAYYRDSFQHGGISMQEML
                     LPLVSLEPL"
     gene            complement(6352..9012)
                     /locus_tag="PLA17_03610"
     CDS             complement(6352..9012)
                     /locus_tag="PLA17_03610"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK84531.1"
                     /translation="MKLRHREYTAAVMVIIALILAGCSAEKNTGASRFYNSLISRYNI
                     WFNGNEAYKAGVKKVRESHRDDYSDLLPVFEYSDAESARMANSDMERAVQKASKVIAL
                     HSITAKPEEKNRRNASSRSEEFYNRREFNEWVDDSYLLMGKAQFYLKNFMAARSSLSF
                     AVSIATDRDLVTEAGIWMARIQTEEGNYTEALRVLQEVTASPESLSRQMKAMYYSTRA
                     DIILRQKKYNEALEPLTAATEYAVNNRTKVRLTYLLAQVCRAAGDNDLSTHYFSKVMK
                     MNPPYDLEFNAGINLAGVTDISHGDADDLFKSLRKMLRDSRNKDYLDQIYFALGELEQ
                     RRGNIDEALKLWSQAAASSTVNTRQKARSYLAIAGHLYQKPDYLKSWYYYDSASYLID
                     NRFPNYEMISRRTSDLGEYAGFHTVVITEDSLRRVAAMSTAERDALIAGMIRKVEEEL
                     TSGRTGDGRDRYNMGQYYENEQRYRGAISAEGGWYFYNQAALTFGRTEFRRRWGDRRL
                     EDNWRRANKTRSAFAPGTIQDAENNGEKTDSAGVAPERTKEYYLRNLPLTDSLLKVSV
                     TRSAEALLGEGKILASRLNDTLSAATSLEEAARTGTDDDIRAEALYELYRLLSKSDPS
                     RAERRRAELLSSYPDSEYALILSDPDYANRQKEMAAKASRSYNAAYLAFTEERFADAI
                     SLCTEAGRLYPEDELLPKFMLLDAMATGALEGELAYKAKLDSLVAKFPTTPEGQRAAE
                     ITDFLRKEKPEIRIAEDTRIAEEIYIADTLQPHYVIIIASNPNANMNQMVFDIINHNL
                     DQYPDRSYRTEGAAIDAGYLLITVGPFEKTADAVTWYRSFNPEQVVREAATAGLTVYL
                     ISRDNLQQFREDKNTDRYAIFHSKAYPNLR"
     gene            complement(9120..10220)
                     /locus_tag="PLA17_03615"
     CDS             complement(9120..10220)
                     /locus_tag="PLA17_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPK84532.1"
                     /translation="MYKKEDILNALRKVNHPEKGTDIVTLGMVGDITQSGEGISLVLV
                     PEKSNDPFIASLRSSCVRALKESLGPDAVIASIDVKPRIVVEKMAEPKRDVLPDVKNI
                     IAVSSGKGGVGKSTVAVNLAVALAREGYATGLLDADIFGPSVPRMFDAGEYRPDIRSE
                     NGTDMIVPMSKYGVKILSIGFFVRDSDAVIWRGPMASSFLKQLISQGDWGELDYLIFD
                     LPPGTSDIHLTLVQEVPVTGAIVVTTPQEVALADARKGIAMFRSEAINVPVLGLVENM
                     SYFETPELPGRRYYIFGREGGSRLATESGTLLLGQIPIVESICDGGDSGRPAALEDNA
                     TGTAFRNLADMVVKQVEIRNAELAPTKRVEMK"
     gene            complement(10564..10920)
                     /locus_tag="PLA17_03620"
     CDS             complement(10564..10920)
                     /locus_tag="PLA17_03620"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPK84533.1"
                     /translation="MKKRSSARVGDNKGFFERVYEVTKLVPYGRITSYGAIARYLGVE
                     GSARMVGWALNSSHTANGYIPAHRVVNRNGILTGKHHFGNSSTMQQLLENENFTVIDD
                     RVVEFRERFWDPSVEL"
     gene            complement(10889..11587)
                     /gene="trmB"
                     /locus_tag="PLA17_03625"
     CDS             complement(10889..11587)
                     /gene="trmB"
                     /locus_tag="PLA17_03625"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPK84534.1"
                     /translation="MGKNKHARWAEMKGFDRVIEPSFEEVFRCDHELKGKWHAEWFGN
                     SNPITLELGCGRGEYTVGLAGLYPAKNIIGIDIKGARMWRGARDAHLMGLPNSTFIRT
                     RIEFTGSLFAPGEIDEIWLTFPDPRMKRAGEKKRLSGPHFLNLYRQYLKDGGIVHLKT
                     DCRELYDYTLALAKANGLAIITSVTDLHREMPGDPLLSIRTHYEEIFLNQGIPITYLS
                     FRLPNEKEIVSPGG"
     gene            complement(11596..12084)
                     /locus_tag="PLA17_03630"
     CDS             complement(11596..12084)
                     /locus_tag="PLA17_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPK84535.1"
                     /translation="MKGGKSKIIAAVLMIAVAAMACDRSIQYSENYSLEKGRWSMFDP
                     AKYTCAIDDTLKIYNIQLSLRTSTDYPYRNIYLFVVTSFPSGTFVTDTIQAMVTDEKG
                     RWLGRGTGDLRELTIPFKSNVYFPEPGEYHFRVIHGMRDTLLNGVYDMGMKISLTGGK
                     AK"
     gene            complement(12065..13210)
                     /gene="ricT"
                     /locus_tag="PLA17_03635"
     CDS             complement(12065..13210)
                     /gene="ricT"
                     /locus_tag="PLA17_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPK84536.1"
                     /translation="MINDDIQAQPGEEQPKNEIKQAIGGANKLNEYNWLSDLPFDERH
                     NEVVEVRFKNTRKGFYRNVNNLKLEVGDIVAVEASPGHDIGYVSMTGFLVNQQLKKMK
                     TRPVIDELKKVYRKARPVDLQKWEEAKSLEVSTMLRSRKIAENLGLDMKIGDVEYQGD
                     KTKAIFYYLADERVDFRQLIKVLAEEFHIRIEMRQIGARQEAGRIGGIGTCGRELCCS
                     TYVTNFISVTTSHARYQDLSLNPQKLAGQCGKLKCCLNYELDCYIDAQKEFPSKEIPL
                     ELAGGTAHFIKMEVHAGIYWYSTDPRITANMTAVPVARVREIIRLNRKGIKPDTLLAK
                     PANNRMERGNDLLLQNSGLDRFESKSRPREGKSRNRPYHRKNERRQK"
     gene            complement(13317..14444)
                     /gene="holB"
                     /locus_tag="PLA17_03640"
     CDS             complement(13317..14444)
                     /gene="holB"
                     /locus_tag="PLA17_03640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPK84537.1"
                     /translation="MNFSQIPGQQAVIDRLRSSVAENRVSHALLFYGPEGSGKFALAL
                     AFARYISCEKRTAEDACGVCPSCVKYDKLIHPDLHFVFPVIKKKQNMEYVSDNYIAQW
                     REMVLRSPWFSLSTWTEAMEVANEQAVIYVAEAAEIIRKLSLKAFESDFKIMIIWLPE
                     KMNAETANKLLKMIEEPPARTLFILVSEEDDRLLPTITSRCQHIRIPSIPANELSAYL
                     VKTMGMDHVRASETAAIANGNMVRAMELAREDDNTTVHLDRFIKLMRTAWTRDIHSLV
                     NWSEETAALGRERQKSFLSYSLRMMRENFILNYNGKENGLVYLTRAEDDFSEKFHPFI
                     NEKNIAAFNHEFNLAYAHVESNGNAKMIFLDLALKTMRHIR"
     gene            14690..15952
                     /locus_tag="PLA17_03645"
     CDS             14690..15952
                     /locus_tag="PLA17_03645"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPK84538.1"
                     /translation="MKTIDNFSFKGLRAIVRVDFNVPLDKTTGVVTDDTRIRGALKTI
                     RKIIADGGSVILMSHLGRPQGVMEKYSLKPVLPVLEKHLGMKVAFAPDALGEEAAAMA
                     AALKPGEVMMLENVRFYPEEEGKPILPETATDEEKKTAKAALKVKQKEMAEKLSTYAD
                     IYVNDAFGTAHRAHATTAVIAGFFPEDKRMFGFLINSELAAMDKVLKSAGKPFTAVMG
                     GAKVSDKILIIENLLDRVDNLIIGGGMTYTFIKAMGGNIGNSLCEADKLDVAKAVIEK
                     AKAKGVSLLLPVDGVNADKFDAEANTYISAIDDVRDGWMGLDIGPKSIELFEKVIMDS
                     KTILWNGPMGVFEMAKFAAGTTAIAKAVAKATENGAFSLIGGGDSVAAINKAGLADKV
                     SYVSTGGGAMLEYMEGKKLPGITAIRGE"
     gene            15952..16488
                     /locus_tag="PLA17_03650"
     CDS             15952..16488
                     /locus_tag="PLA17_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84539.1"
                     /translation="MTRRVIPVVALMMMFAGCGGNDQKTSGTITLSSDLYDGGSYYYA
                     MGLSFDEAGEVPTLPDQYRADIILGAGPVEGGPVVAYLTANTYKPPFALAGTYDSESL
                     AKSAFSELKSVGSYSWVEFASPLAANQIWVVKTRDSKYAKLRIISVILNTSVTPAAAT
                     CTLEWVWQPDGSSTFPAK"
BASE COUNT         3716 a         4298 c         4327 g         4186 t
ORIGIN      
        1 aagggtgaag aagagagcca tggcaatgat ctcggcggcc acccagatga gataatgcca
       61 gatgagaagg ctctttccgc gtttgacgtg gtggtacatc agaatacggc ttattgccac
      121 caccagcacc cccgcgagga tgacaccgct ggaatagaac agcagggtgg gcttcgaaac
      181 ctcaaaccag gtacttacac caaagggtgt gtacaggttg aggaagagaa gtgcaaagcc
      241 agccgtgaaa agtatcgtgg taacgatatt ccccttttcc gtcaggtacg cgggcagttt
      301 tttgttcagg tcaatattca tggtatctat tgcaaaaata atacttttac cgacctgctg
      361 tttaaggtac tgaaaaggga gtcgattccg ggagacatat aaccagtaac tgttccgggt
      421 gctgaaaagt gagtcaattc cggaagaagt gtaaccagta actgttccgg gtgatgaaaa
      481 gagagtcaat taaggaagaa gtgtaacaag caactgttcc gggcggttga gagcagaact
      541 taaaaaggcc tgtcaggcat cattccggca tgaatcatgg cagaaatttt cacccctgaa
      601 tgctctgttt cgcccctgcc cggagaattc cggcccgctg gcgggtgcaa gacacctaat
      661 ttttggagtg ccgcttactc tctgttttgt ttgctccaaa attcagctat gggttatacg
      721 gcaacagaac ggcaaatctc ctcacacttc agggagggct tggcggaagg agtgattccg
      781 ggaaccgggg taactgtctc tgtccggaga ttcagctctg ccgttgacag ctcccttcgt
      841 gacgggcaga atgtgccggg cctgtttcgt tctcttttcc gctcacgcga ggtggaagag
      901 agcaacaggg agttcagtta ttatatcctg agtccttgtg tcgacagtag taaaccatgc
      961 gacggcagcg gtgcaatcat actgcttcac gggctaaacg aacggaactg gtcaaagtac
     1021 attcagtggg gtgtatcgct ggcagacagg actgggcgcc cggtggtgct gttcccgctg
     1081 gcctatcaca tgaaccgctc accgcgcagc tggattgaca ggcatatcat gatgccactg
     1141 gtctcagccc ggcaggcgct gatgcctgac gccaggctct ctacttttgt gaatgtggct
     1201 ctgagtaccc gcatgacagc ctcgccccag aggttcatgc tctcgggata tcagaccgca
     1261 cgtgatctta ctgcgctgat ccgttccatg agggagggca gcatccccgg gatagctgcc
     1321 ggcgggcgga cagatttttt tgcctactcc ataggagctc tgctgatcca ggtcatgatg
     1381 ctctccgata cggcactgct gcctgatgac agccgcatca tgctcttctg cggcggcagc
     1441 gctttcagca tgatgaacgg aacctcgaag ctgataatgg acagccgcgc tttcgagatg
     1501 ctgctctcat tttatatagg ctggcctgag agtatcatga atggcgggag gaaacgactt
     1561 acccggttaa tgaatgatac ccccgagggg agagcgttct ttgccatgac cacgctgcag
     1621 cgactggaaa atctgcgggg aagaccattc aaccgatatg atggcaggct gaaagcggtg
     1681 acgtttgaat cggatactgt cattcctgca aaggctgtta tggagacact cagcggtgca
     1741 gatactgagg tatgggatcc gggatatccg tgtactcatg agtcaccctt cccggttctt
     1801 gccggggcca atgaaatcaa agttgaccgg acctttgacc ggctctttga taccgctgcg
     1861 cgattttttg catgaaatga ctatttttga tacttaaccg tatcaacaga gtctatgaaa
     1921 tcacttttga ttccgccata cctcagggag ggcgaccgta ttgagatcat cagccctgcc
     1981 agtcatatcg aagagggcgt ggtacacagt gccgtacaga tgcttgagga atcaggcttc
     2041 cgtgtcaccc tgggcagcca tgtttttacc cgcagcggcc catttgcagg aaatgaggca
     2101 gagcggctgc atgacattca acaggctacg gatgacccgg atgtaaaggc tgttatctgt
     2161 tcacgcggcg gttatggcat gtcaaggatt gttgaccgga tcgacttctc agctctgaaa
     2221 aagcagccta agtggtatgc gggctacagc gatatcactt cactgcacat gtggctcaac
     2281 cgggtatgcg gcattgcctc gctgcatgcc gagatgccgc taaactactc caattctgaa
     2341 taccttccgg tatgctatga caccctggta aaagcactgc ggggcgaagc tgaaccggta
     2401 cgctggctgc ctctgcatga ggcctcgctt gaggtctccg gccacgtcac cggtggcaac
     2461 atatcgctga tatacagcct caacggaaca cctgcgcagc ctgacacaga gggggcaatc
     2521 ctgttcatag aggaaatagg ggagcgcttc tatcatctcg acaggatgct ggtagcaatg
     2581 aggatggcag gagtgctgag aaacctgtcg gcactggtgg taggaggtat ggagaagata
     2641 acggaggggg agcacgtgtt taaccagacc attgaggagg tggtgatgaa tgtggtggca
     2701 gggtatgatt atcccgttct cttcaacttt ccggcaggac atatacctga caacagggca
     2761 atttacctgg gaaggaatgc aaggatcaca cagtcaggca gtgaagctgt catgagctgg
     2821 atctgatctg tggcgggccg gctcgatctg atccatgacg ggccggctcg atctgattcg
     2881 tagccggatt gactcaatcc gatctttggc gggccgagtc gatttgatcc gccacaggct
     2941 gcaatgcttt atggcctgca gccattatta ctactgcctg ctgatcagaa cgctgccagc
     3001 agcaggttga tatcctgcga aaggataccg aacttctggc ccagcacatc attggtgaga
     3061 ataccattgt agatatagac accgctccgt agtccgggat tgaatctgag catatgggta
     3121 atgcttcctg cgtcagccat ctcctgcatc agtggtctga agacgttgct cagtgctatg
     3181 gaggctgtct tgggcacccg cgaaggcagg ttccaggcgg agtagtgtat cactccatgc
     3241 ttctcataga tcgggtcttt tatcgccctc ggctcagccg tctccacaca gccgccggtg
     3301 tcaacactga gatcgatgat aaccgaaccg cgcttcatcc cctgcaccat ctcctcggtg
     3361 acgtaaaacc agggcatgag tatatctgat gagagggctc ctatgagaac gtcagctgac
     3421 ttgagcgcct tctgcagcac ctgcgggtga aatgtagagg tgtatattct ctgccccagc
     3481 aggttctgaa agttacggag gcgatgcacc gatgcgtcaa acactctcac gatggctccc
     3541 agccccagcg ctgcccgtgc ggcatactcg ccggccgtat tggcgccgat tatgatcacc
     3601 tccgtgggtg tcactccggt cacacctccg agcatcactc ccttgccccc ggaggcggag
     3661 ctcagatatt cggatgccag gagtactgat gtaatgccgc tgatctcact cattgactca
     3721 accaccggaa gcatcccgtc ggtatccttt atcttttcgc ttgccagggc cgtcaccctc
     3781 ttgcgcatga gtgtcgaaag aacatcctcg tccatggtgg cgacattcat gaatgagatg
     3841 accgtctgcc cgacttttag ctgtgaggct tcattctgtt tgaagggagt tatcttgatt
     3901 ataatgtcac actcatatat cctggatgga gtgtcagcca caattgcccc attgtcgctg
     3961 tattccctgt cggtgaaacc ggcagcctgt ccggcacctc tctccatgaa gatctcgttg
     4021 ccggcatcgg taagaatcct gattgcttca ggggttagtg gtattcgttt ttcatcatca
     4081 tgaacatcac gggggagacc aatcgacatc tgcttcctgc ccacagcttt ttcaatctgc
     4141 tcttcccttg gcaggagagc gcttctggcc ggttcattca tctggcttcg ttttaaaagg
     4201 ttaagagtga ggaaaaaact taccagatca aaattaagat tttaattgta cagatgacag
     4261 aatgaagagg ataaatcaga gacggtttcc tgcaaagtta acagggcgat tctgtcnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgg cagcagcggt tcgatgagtt
     4441 caggccactc catgaagcat ggtgcggcgg aatcaaaata ttcctcgatg ccgaagtcga
     4501 gcacctcggt aatcttcttt atcctgaaaa agtcgaaatg ataaacaggt gtactgcccg
     4561 gcctgcgata ctcgttgacc agtgtaaagg tgggactggt gacggtgtct gccacgtcca
     4621 tctcatggca cagtgccctg atgaaagttg tctttccgga gcccatctca ccgtaaaagg
     4681 caaagagtct tttcccggct gtctccttca ggaaatgcct tgcagcatca tgcagtcggc
     4741 tcttgtcggc aataacgatt ttcatgctgc aataatggca aatcagagcg gttcaaggga
     4801 gaccagtggc agcagcatct cctgcatcga aatgccgcca tgctggaatg agtcgcgata
     4861 gtaggcaaca acgctgttgt agttgttcgg gtagaccagg aagtcatttg cgcaggcgaa
     4921 aatatacctg gaggttatgt tcaccatggg caatcctgcc tttgccggtg ctgtgatctc
     4981 aaatacttcg cgcgggttat agtcgaggtt cctgcccatc ttgtaacgca gattcatcga
     5041 tgtctgcctg tctcccacca ctttaagcgg ccgctgcacc ctgaccgatc cgtggtcggt
     5101 ggtgaatacc acccgtgccc cacgggatga gacgagcctc agcagttcca tcagcggaga
     5161 gtggatgaac cacgagtgtg tcagcgtgag ccaggaggcc tcgtcagcag tcaggtcacg
     5221 gatgaggttg atctcggtcc gggcatggct cagcatgtca acaaagttgt ataccagcac
     5281 gttaaggtca ttgcccataa gctgtgcgat acggtcgttg acgcttctgc cctcagcgtc
     5341 ggaattgatc ttgttatagc tccaccggca attgataccg gctctgagca ggttctttga
     5401 caggagctgc tcttcgtgca ggtttttgcc ttcatcctca tgatcatcaa tccagtattc
     5461 aggcatcagc tcctcgatgg cagccggcat cagaccggca aagagggcgt tgcgtgaata
     5521 ctgcgtggtg gtggggagga tactcaggta gagctcttcg tcaacaatgc gcatcacctt
     5581 tcgcagatcg cctgcaatgg tctgccactg gtcgaggcgc atgttgtcaa tgatgatgaa
     5641 aaagagcgga gtctccctgc tgatcatggg aaaaattctc tttgacaaca cggctggcga
     5701 catcaggggc ctgtcagccg ccccggcgga aacccacttc atgtagttgc cggaaatata
     5761 ttttgcgaaa gccctgttgg catctgactc ctgcatgcga agcacctcgc gcataccggt
     5821 atccggtgac ctgatcagtt ctccctgcca gaaggtgagc cggcggtaga gctcgctcca
     5881 ctgttcaaag gtggaggctg acgagatcat gtcagcaatg ctgctgaact cttcccggaa
     5941 gacagtagtg gtctgccttg ccacaatctc ctcgatattc agtatacgct taagagtcag
     6001 cagaatctgc tgcggcttga cgggcttgat aaggtaatca gctatctgtg agccgatagc
     6061 ggcgtccatg atatcctcct cctcgctctt ggtgatcatg acaacgggga tggcaggcct
     6121 tatggccttt atccggcgca gggtctcaat accgctgagg ccaggcatat gctcatcaag
     6181 gaagatgatg tcataggcct tctccctgac cattgcaatt gcatcattgc ctgaatggca
     6241 ggtgtcagtc tcaaaaccct tttcttcgag gtaaaggata tatgacctga gaactcctat
     6301 ctcatcatct acccagagaa ttcgtatctg tctcatttgc agcttttcag gttaccgaag
     6361 gttgggatac gctttcgagt gaaagatagc atatctgtcg gtattcttgt cttcccggaa
     6421 ttgctgcagg ttgtcgcggc tgataagata taccgtcagg ccggcggtgg ctgcttccct
     6481 gacaacctgt tccgggttga atgatctgta ccatgtcact gcatcggctg ttttttcaaa
     6541 tggcccgacg gtgataagaa gatatccggc gtctatggca gctccttcgg tgcgatagct
     6601 ccggtcaggg tactggtcaa ggttgtggtt gatgatgtca aacaccatct ggttcatgtt
     6661 tgcattggga ttggaggcaa tgattatgac ataatgaggc tgcagtgtgt ctgcaatgta
     6721 aatttcctcc gcaatgcggg tatcttctgc tatcctgatt tccggtttct ccttgcggag
     6781 gaagtcggtt atctccgcag ccctttgccc ttcaggtgtt gtgggaaatt tcgccaccag
     6841 cgagtcaagt tttgccttgt aagccagttc gccctccagg gcgcctgtgg ccatggcatc
     6901 gagaagcatg aatttaggca acagttcatc ttcgggatag agtcttccgg cttcggtgca
     6961 gaggcttatt gcgtcggcaa atctctcttc ggtaaatgcc aggtaagcag cgttatagct
     7021 tcgcgaggcc ttcgcagcca tctccttttg tctgttggca taatcaggat cagacaggat
     7081 gagggcatat tccgaatcag gatatgatga cagcagttca gctctgcgcc tttcagccct
     7141 ggacgggtcg cttttactca gcagccggta tagttcgtag agagcttctg ccctgatgtc
     7201 gtcatcagtt cctgttctgg cagcctcttc aagtgaggtg gctgcagaga gagtgtcgtt
     7261 gagccttgat gcaagtatct tgccctcacc aaggagcgcc tccgcgctcc gtgtcacaga
     7321 gactttgagg agcgagtctg tgagaggcag gttacggaga tagtactcct ttgtccgttc
     7381 cggagccact ccggcgctgt cagtcttttc cccgttgttc tcagcgtcct gtattgttcc
     7441 gggggcgaat gctgatctgg tcttgtttgc cctcctccag ttatcctcga gtcttctgtc
     7501 tccccagcgg cgcctgaatt cagtacgtcc gaaggtcaga gccgcctggt tgtagaaata
     7561 ccatcctcct tcggccgaaa tagctccgcg gtatcgctgt tcattctcat aatactgccc
     7621 catattgtac ctgtctctgc cgtcaccggt tcgcccgctg gtcagctcct cctccacttt
     7681 tcttatcatg cctgcaatca gggcatctcg ctcggcagtg ctcatggcgg ccaccctgcg
     7741 gaggctgtct tcggttatca ccacggtgtg gaagccggca tattcgccaa ggtcagaggt
     7801 cctgcggctg atcatctcat agttcgggaa acggttgtct atgaggtatg aggcgctgtc
     7861 atagtagtac catgacttca gataatcagg cttctggtat aagtgtccgg caatggcaag
     7921 gtatgaacgt gccttctgcc tggtgttgac agtgctgctg gctgctgcct gtgaccatag
     7981 cttcagggct tcgtcaatgt tgccccggcg ctgttcaagc tcacccaggg caaagtatat
     8041 ctgatcaaga taatccttgt tcctggagtc gcgaagcatc ttacgcagtg atttgaaaag
     8101 atcgtcagcg tcaccatgcg aaatatctgt cactcctgcc aggttgatgc ctgcattgaa
     8161 ctccaggtca tagggcggat tcattttcat cactttgctg aagtaatgtg ttgagaggtc
     8221 attgtcaccg gcagccctgc agacctgggc aaggagatag gtcagacgca cctttgtcct
     8281 gttgttcacg gcgtactccg tggcggcggt gagaggttca agagcctcgt tatacttctt
     8341 ctgcctgaga atgatatctg ccctggtgga gtaatacatt gccttcatct gacgtgacag
     8401 actctctgga ctggctgtga cctcctgcag cacgcgcagc gcttcagtat aatttccctc
     8461 ctccgtctga atgcgggcca tccatatacc ggcttcggtg acaaggtcac ggtcagtggc
     8521 aatgctcacg gcaaatgaga gtgatgagcg ggctgccata aaattcttca ggtagaactg
     8581 cgccttgccc ataagcaggt aggagtcatc aacccattca ttgaattccc tcctgttgta
     8641 aaactcctca ctcctggagg atgcattcct ccggttcttt tcttcaggct ttgcggtgat
     8701 ggagtggagc gctatcactt tggaggcttt ctgaacagca cgttccatgt cggaatttgc
     8761 catgcgggcc gactccgcat cggagtattc gaaaacggga aggaggtcac tgtagtcatc
     8821 gcggtgcgac tctctcacct tcttcacacc ggccttgtag gcttcattgc cgttgaacca
     8881 gatattgtac ctggagatca gtgaattgta aaaacggctc gcaccggtgt tcttttcagc
     8941 agaacacccg gcaagaatca gagcgataat gaccattacc gccgcagtgt attccctatg
     9001 ccgtaatttc aacaaaaatg gtttatactc taataactga tgaggcactc tgaaagtatc
     9061 caaagttaag aaaaagaggc gggaaaaaag gtgccagact accgggggcg gcagcatgct
     9121 tacttcatct caaccctctt cgtcggggca agctccgcgt tgcgtatctc cacctgtttc
     9181 accaccatat cggcaaggtt cctgaaagca gttcctgttg cgttgtcttc cagcgccgcc
     9241 ggcctgccgg agtcacctcc gtcacagatg ctctccacaa tgggtatctg ccccagcagc
     9301 agggtgccgc tctctgtggc aagcctgctg ccgccttccc tgccgaagat gtagtatctc
     9361 ctgccgggaa gctcgggagt ttcaaagtag gacatgtttt caacgagacc cagtacaggc
     9421 acgttgattg cctcgctgcg gaacatcgct atccccttcc gcgcatctgc cagcgccacc
     9481 tcctggggtg tggtcaccac aatggctcct gtgacaggca cctcctgaac aagtgtcagg
     9541 tgtatgtcac tcgtcccggg cggcaggtcg aagatgagat aatcaagctc tccccagtcg
     9601 ccctggctga taagctgctt caggaaactg ctggccatgg gcccccgcca gatgacagca
     9661 tcagaatccc tgacgaagaa acctatggag aggattttta cgccatactt tgacatcggg
     9721 acaatcatgt ctgttccgtt ctcactccgt atatcgggac ggtattcgcc ggcatcgaac
     9781 attctcggca ccgaggggcc gaagatatcg gcgtcaagaa gtccggtggc ataaccttcc
     9841 cgagccagcg ccactgccag gttcacggcc accgttgact tgccaacacc acccttgcct
     9901 gaggagactg ctatgatgtt cttcacgtca ggcagcacgt ctctcttcgg ctctgccatt
     9961 ttttcaacca ctatcctcgg tttgacatct attgatgcga tgaccgcgtc agggccgaga
    10021 gattccttca gagccctcac gcagctgctc ctgagggatg caatgaacgg gtcatttgat
    10081 ttttccggca caagcaccag ggagattccc tctccggatt gtgtaatgtc acccaccatt
    10141 ccgagggtga cgatgtcagt acccttctcc gggtggttga ccttgcgaag ggcgttcaga
    10201 atatcttcct ttttgtacat gttattttgt tatgattaat gatctggtct gtaatgctga
    10261 atcctttccc cggcgctctg cggctgccgg gtcaggctct gcaggcaata tagattctcc
    10321 tcctttgatt tgcaatgcat tttgctctcc tgttcctgcc acagcttccg ggtcaggctc
    10381 tgcgggcaat ataaattctc ctcctttggt ttgcaatgtc ttatgttctc ctggccctgc
    10441 cacagcttcc gggtcaggct ctgcaggcaa tttgaattct tgtcattagg cctgcgatac
    10501 attatgttct ccctggctac gtcactgctc cctgcttctt tcattatcaa cacaatctgt
    10561 catctatagt tcaacagagg gatcccagaa ccgctccctg aattcaacca ccctgtcgtc
    10621 tatcaccgta aagttttcat tctccaataa ttgctgcatc gttgatgagt tgccgaagtg
    10681 atgcttaccg gtgagaatac cgttgcggtt taccacacgg tgtgccggga tatacccgtt
    10741 agcggtgtgc gatgaattga gggcccatcc caccattctg gcggagccct ccacgcccag
    10801 atatctggca atggcgccgt atgacgtaat ccggccatag ggcaccagct tcgttacttc
    10861 gtacacacgc tcgaagaagc ctttgttatc acccacccgg gctgacgatc tctttttcat
    10921 ttggcagtct gaatgacaga tatgttatcg ggataccctg attaaggaaa atctcttcgt
    10981 aatgtgtcct gattgagagc agcggatcac ccggcatctc ccggtgaagg tcggtgacgg
    11041 atgtgattat tgcaaggccg tttgcctttg caagcgcgag tgtataatca tacagttcac
    11101 ggcagtcggt cttcagatgc actatgcccc cgtctttcag gtattgcctg tacagattca
    11161 ggaaatgcgg gcctgaaagc ctcttctttt cccctgccct cttcatccgg gggtcaggga
    11221 aggtcagcca tatttcgtct atttcgccgg gggcgaagag cgagcctgta aactctatgc
    11281 gcgtacggat gaatgtggaa ttaggaagac ccatgagatg ggcatccctg gcgccccgcc
    11341 acatacgcgc ccccttgatg tctatgccga ttatattctt cgcggggtag aggcctgcaa
    11401 gccccacagt gtattctccc ctgccgcatc cgagctccag ggtaatggga ttgctgttgc
    11461 cgaaccattc cgcgtgccac tttcccttaa gttcgtggtc acacctgaac acctcctcaa
    11521 aagagggctc gataacacgg tcgaaccctt tcatctccgc ccaccgggcg tgtttgttct
    11581 tgcccaaaat ccaggttatt ttgctttccc gcctgtcagc gaaatcttca tccccatgtc
    11641 atagacacca ttcaggagtg tgtccctcat accgtggata accctgaaat gatattcgcc
    11701 cggctcagga aaatatacat ttgacttgaa ggggatggtc agttcacgca ggtcacccgt
    11761 ccccctgccc agccacctgc ctttttcatc ggtcaccatg gcctggatgg tgtcagtcac
    11821 aaacgtgcct gaagggaagg atgtaacaac aaagaggtag atgttccggt acgggtagtc
    11881 ggtcgaggta cgcagtgaca gctgtatgtt gtatatcttt aatgtgtcat caatggcaca
    11941 tgtgtatttt gccggatcaa acatgctcca tctgcctttc tccagactat agttttccga
    12001 gtattgaatg ctccggtcgc atgccatggc agccactgca atcatcagca cggctgctat
    12061 gatcttactt ttgcctcctt tcatttttcc tgtgatatgg tctgtttctg ctcttgcctt
    12121 cacgcggacg gctctttgac tcaaacctgt caagcccgct gttctgcagc agcagatcat
    12181 tgcctctttc catgcggttg tttgccggtt tggccagcag ggtgtcaggc ttgatccctt
    12241 tacggttcag cctgattatc tcgcgtaccc gtgccacagg cactgccgtc atgttggcgg
    12301 taatccgcgg atcagtcgaa taccagtaaa tgccggcgtg cacctccatc tttatgaagt
    12361 gggctgtgcc tccggcaagt tccaggggta tctccttcga gggaaattcc ttttgcgcat
    12421 caatatagca gtcaagctca tagttcaggc agcacttgag tttgccacac tgcccggcaa
    12481 gcttctgcgg attgagggag aggtcctggt agcgtgcatg tgatgtggtc accgagatga
    12541 aattcgtcac ataggtagaa cagcacaact ccctgccaca ggtgccaata cctccgatgc
    12601 gccctgcctc ctgccgggca cctatctgcc tcatctctat tcttatatga aactcctcgg
    12661 caaggacctt gataagctgg cggaaatcaa ccctctcatc ggcaaggtag tagaagatgg
    12721 cctttgtctt gtcaccctgg tactcaacat caccgatctt catatccaga ccaagatttt
    12781 ctgcaatctt cctcgagcga agcatggtgg aaacctcaag actctttgcc tcctcccatt
    12841 tctgaaggtc aacaggcctg gccttacggt agaccttctt cagctcatcg atgacaggcc
    12901 ttgtcttcat cttcttaagc tgctgattga caagaaaacc ggtcattgac acataaccga
    12961 tatcatgtcc gggtgaggcc tccactgcaa caatgtcacc aacttccagt ttcaggttgt
    13021 tcacattgcg gtagaaaccc tttcttgtgt tcttgaagcg gacttcaacc acctcattgt
    13081 ggcgttcgtc gaagggcagg tcggaaagcc agttgtactc attcagcttg tttgcccccc
    13141 cgattgcctg ttttatttca ttctttggct gctcttcacc gggctgggcc tgtatatcat
    13201 cgtttatcat aacatcttgt taaccgctgg cggaagtaaa gatcaatttc atcctttccg
    13261 taaaggtgaa tacaaaatta ggaataaaag agctattgtc aagtgattcc tttcagttac
    13321 ctgatatgtc tcatggtctt cagggccaga tcaaggaaga tcattttggc attgccgttg
    13381 ctctcaacgt gggcgtatgc aaggttgaac tcatggttaa aggcagcgat gttcttctcg
    13441 ttgatgaagg ggtggaattt ttcagaaaag tcatcctcgg cgcgggtgag gtaaacaaga
    13501 ccgttttcct taccgttgta atttagtatg aaattttcac gcatcattct cagggaatag
    13561 gagagaaagc tcttctgtct ctcccttccc agcgctgcag tctcctctga ccagttcaca
    13621 agcgagtgga tgtcacgggt ccacgccgta cgcatcagtt ttatgaaccg gtcaaggtgt
    13681 accgtggtgt tgtcatcttc gcgggcaagt tccatggccc tgaccatgtt gccgttcgca
    13741 atggcggctg tctccgaggc cctgacatga tccatgccca tggtctttac caggtatgca
    13801 ctcagttcat ttgctggtat tgaaggaatc ctgatgtgct ggcatcgcga ggtgatcgtc
    13861 ggaagcagcc tgtcatcctc ttcggaaaca agtatgaaga gggtgcgggc agggggctcc
    13921 tctatcatct tgagaagctt gttcgccgtc tcggcattca tcttttcggg aagccagatg
    13981 atcattatct tgaagtcaga ctcaaatgcc ttgaggctga gctttcggat tatctccgct
    14041 gcttcggcca cgtatatcac ggcctgttcg ttggccacct ccatcgcctc tgtccatgtg
    14101 ctgagcgaga accatggtga ccggagcacc atttcgcgcc attgcgcgat atagttatca
    14161 ctgacatact ccatgttttg tttcttttta atgaccggga agacaaagtg gaggtcagga
    14221 tgtatcagct tatcatactt gacgcatgac gggcaaacgc cgcaggcatc ttcagcggtg
    14281 cgcttctcgc agctgatata ccttgcaaac gccagcgcca gggcgaactt gccgcttccc
    14341 tcgggaccat aaaagagcag ggcatgactg acgcggttct ccgccacgga gctccgcagc
    14401 ctgtcaatca ctgcctgctg tcccggtatc tgtgaaaagt tcatattgcc tttcggatga
    14461 cgaagagcaa atttatacaa aaatcagaaa atatctgcct ttcagagcgc cgcgggtgaa
    14521 ctgtcatggt caggctcctg cacgaagatt cccgtattta acaaatcacc gtggtatttg
    14581 ttaaataaaa aataaattgc cattattaac cctcctgcat gcgaggaacg cttcaacttc
    14641 ttatttttgc acaatacaaa cgaacagtta atttataagt ataaattcta tgaaaaccat
    14701 tgacaatttt agttttaagg gcctcagggc aatagtacgt gttgatttca atgtgcccct
    14761 tgacaaaacc acaggcgtgg ttacagatga cacccgcata cgcggagctc tcaagacgat
    14821 aaggaagatt atcgctgacg gcggaagtgt cattctcatg tcacacctgg gccggccgca
    14881 gggagtaatg gagaagtatt cccttaaacc agttctcccg gttctggaga aacacctggg
    14941 gatgaaggtt gcttttgctc ctgacgccct tggcgaagag gccgcagcga tggctgccgc
    15001 cctcaagccg ggcgaagtga tgatgctcga aaatgtcagg ttctatcctg aggaggaggg
    15061 caagccgata ctcccggaaa ccgccacgga tgaggaaaag aaaactgcaa aggcagccct
    15121 gaaggtgaaa cagaaggaga tggctgagaa gctttcgacc tatgctgaca tatatgtcaa
    15181 cgatgcgttt ggcacggctc acagggccca cgctaccacc gccgttatag ccggcttttt
    15241 cccggaagat aaaaggatgt tcggcttcct tataaacagc gagcttgctg caatggacaa
    15301 ggtgctgaag agcgccggca aaccgttcac tgcagttatg ggtggtgcca aggtgtcaga
    15361 caagatactc atcatcgaga acctgcttga cagggtcgat aacctgatta tcggcggagg
    15421 catgacatac accttcataa aggctatggg aggaaacata ggcaattccc tgtgtgaggc
    15481 cgacaagctt gatgtggcaa aggcggtcat cgaaaaggca aaggcaaaag gggtcagcct
    15541 gctcctcccg gttgacgggg tcaatgctga taagtttgat gctgaggcta atacatatat
    15601 ttcggcaatt gatgatgtga gggacggatg gatgggcctt gacatcggac cgaagagcat
    15661 tgagcttttt gaaaaggtga ttatggattc aaaaaccatc ctctggaacg gcccgatggg
    15721 ggtgtttgag atggctaagt ttgccgccgg aaccacagca atagcaaagg cagtggcaaa
    15781 agccaccgaa aacggagcat tctcactgat aggcgggggc gactctgttg cagcaataaa
    15841 caaggccggc ctggccgaca aggtgagcta tgtctctacc ggcggaggcg ccatgctgga
    15901 atatatggag ggcaaaaagc tgccgggcat cacagcaatc aggggtgaat aatgaccaga
    15961 cgggtcatac cggtcgtggc cctgatgatg atgtttgcag ggtgcggagg caacgaccag
    16021 aaaacctcgg gaacaatcac tctttcgagt gacctgtacg atggagggtc ttattattat
    16081 gccatgggac tctcctttga tgaggccggc gaggtgccca cgcttcctga tcagtaccgg
    16141 gctgatataa tactgggcgc tggtccggtt gaaggcgggc cggtggttgc ataccttacg
    16201 gcaaatacat acaagccccc attcgccctg gcgggaacat atgatagcga gagcctggcg
    16261 aagagtgcct tcagcgaact gaagagcgtg gggagttatt cgtgggtgga atttgcatca
    16321 ccgcttgccg ccaaccagat atgggtggta aagacacgcg actcgaaata tgccaagctc
    16381 aggattatca gcgtgatact caatacatcg gtcactcctg cagctgccac ctgcaccctt
    16441 gagtgggtgt ggcagcctga cggatcatca accttcccgg cgaaatgatg gaattcccgg
    16501 aagagatatt caggatctcc ggcgccagct catttgaaaa gctggcactg gagatctttc
    16561 gcttccaggc tgccgggtgc ccggtctaca gtgaatatat aagccttctc ggaatcaaac
    16621 cggaaga
//
LOCUS       DAOPBK010000038        16526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3559_length_16526_cov_5.700079, whole genome shotgun sequence.
ACCESSION   DAOPBK010000038 DAOPBK010000000
VERSION     DAOPBK010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3559_length_16526_cov_5.700079"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLA17_03655"
     CDS             <1..580
                     /locus_tag="PLA17_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPK84540.1"
                     /translation="VWLDPAKTTPYQFYQFWLNVADADAEKFIRIFTMLPQSEITGLA
                     EAHQAEPERRILQKRLAEEVTVMTHSREEYEAAVEASQILFGKGTTEQLRKMNENTFL
                     ALFEGVPMFDISAGLLEAGVTLVDLCAVHTAITESKGEFRRLISGGGVSLNKSKMDNA
                     EMIITREHLLNNKYILIQKGKKSYFLIRVKEE"
     gene            619..1506
                     /locus_tag="PLA17_03660"
     CDS             619..1506
                     /locus_tag="PLA17_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPK84541.1"
                     /translation="MKGNIRAEFRQDSFDLLNFFIRHFRKFVIAGIVAAILSAVISLL
                     IRPLYESTVILYPSSNITEATSLLGETESRTALFGDDDATEKLIQVINSAQVREYLKE
                     KYNLMQHYNIKPGEKYPNTLMKAKMDKYMRCTRTDYGSVKIRVRDRDRVYACNMANDM
                     AARADTIFNNLQREAAMVTLDEISRLYDIQKRLVRQYEDSLMNLSSAAALRLYSTFEM
                     ENDYLGLMRGRFLEAQALSRQTLPYILVVDRAVVAEKKVFPKRTVMVLLSTVSVLLLL
                     AMVLFIAEGVKLRRADDRQ"
     gene            1493..2875
                     /locus_tag="PLA17_03665"
     CDS             1493..2875
                     /locus_tag="PLA17_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK84542.1"
                     /translation="MTGNELLILIIPAVFAILLLALLSLEYLLLLTLFLTPLSLQLSY
                     LTGSTGFDLSLPTEPVMAFLLLTVLFRLTVTREFPKELLRHPMTWMAGLYLLWTFITA
                     LTSTIPGVSFKTLAYRLWFMAGFYLVTARLAFVERFRKRYILAYAAGLAVVVIYFIIR
                     VQGAGLLNQQFAHSACYPFYKDHTSFGASMAFIIPPLTVMLFSRGSLWHRGFLAALLL
                     IFVAGFVLSYSRAAWVSLIAASALSLILWTRMPVKLLSLSALLFVIVLAFSAGSIWQK
                     MDSTTEDSSTDLGQHLRSSSNITTDQSNLERINRWKCALRMFAEKPNTGWGPGTYQFC
                     YAPFQKPSERTAISTDFGDAGNSHSEYLGALSESGWPGALIFLAMTLCAVITGIKVWY
                     REGRGEKGYFALAVVTGLVTYMVHGVMNSFLDSDKIAALWWGFIALIVAMDLDGQRIA
                     GAPESDSSPA"
     gene            complement(2993..4225)
                     /locus_tag="PLA17_03670"
     CDS             complement(2993..4225)
                     /locus_tag="PLA17_03670"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPK84543.1"
                     /translation="MKTREGRPPKDRLKMSKFKLDALLDITLSINANLPTTTLLGKYE
                     SILRDELGIGKILLFKYGEQWEPLLSSGFPESLKESVDVENDLLHFREIQTEVPQERF
                     RAVDFIIPVLHNNEPSAYVLIGEIDEEREGMSPLIKHLHFIQTISGIIVVAIENQRLF
                     NESLRQEALRRELELAARMQMKLIPNNTKLPSDNLIRAKGFYFPHYEVGGDYYDFMQL
                     SEEIAGFCIADISGKGMSAAILMSNFQASLRALFTANASFEELIRTLNTLVVTNSDGE
                     GFITLFIARYNTRSRELEYVNAAHTPPLIINLSTGQSSLLRPTCVGVGMIDDIPCVKT
                     ETLTLKNPTKIICYTDGLSELKDEGGRDYGIAPLVKHFPNRKPLADNIESLISDIDIS
                     SNNQRLFDDVSILAIEFP"
     gene            complement(4227..4793)
                     /gene="efp"
                     /locus_tag="PLA17_03675"
     CDS             complement(4227..4793)
                     /gene="efp"
                     /locus_tag="PLA17_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPK84544.1"
                     /translation="MATTADFRNGMVIEFNDDLYTIVQFQHVKPGKGPAFVRTKLKNV
                     TTGKVLDNTFSSGVKVNVARVERRPHQFLYSDDMGYHLMHVETFDQITVPHSMIENSD
                     LLKEGDTVEVVVHADTENVLLVDLPVSVVMEVTYSEPGLRGDTATNTLKPATIETGST
                     IMVPLFVNAGDKIRIDTRDRSYIERVKG"
     gene            complement(4816..5595)
                     /locus_tag="PLA17_03680"
     CDS             complement(4816..5595)
                     /locus_tag="PLA17_03680"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPK84545.1"
                     /translation="MKKILIAALIAITAALQLLDAQKQPYHPGEKVTYQIKYGIVGSG
                     QASLELSEGFHKGQPVWHAVISGKTTGLADALYRVRDVYESYIDPENDLPVFSIRNIQ
                     EGRYRKYNEVGFDHMSETDSTLVFSDLTGNHKGPKGLLDIVSCFYWFRKYYLASGKEL
                     VPGETYKMMTWFADELYPIILRYKGKETVRVRGKRIECYRFHPVTEVGRVFKTEEDMA
                     MWFTTDENFLPVKIRFDIFVGSFYVDMVSYEGLRAPLSFID"
     gene            complement(5599..6303)
                     /gene="tsaB"
                     /locus_tag="PLA17_03685"
     CDS             complement(5599..6303)
                     /gene="tsaB"
                     /locus_tag="PLA17_03685"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPK84546.1"
                     /translation="MILCIETATSVCSAALCDETRVHGLIEAPQGMSHASQLTVIIQQ
                     LMKENRITATDLDAVAVSKGPGSYTGLRIGVSVAKGIAYGAGIPLIGIDTMAAMCSGY
                     ISLHPEDLLPGTLLCPMIDARRMEVYNALFAADGTQVMETSASIIDKDSFSEFLSENR
                     VIFFGNGSGKCRGIITGPNALFRDDFSLSASYLLIPSVNAVRNRQFEDLAYFEPYYLK
                     EFIATVPKSLFPDKPE"
     gene            complement(6325..7593)
                     /locus_tag="PLA17_03690"
     CDS             complement(6325..7593)
                     /locus_tag="PLA17_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK84547.1"
                     /translation="MNNNRLLKTLILAVILLLILAVVGKKAGWFGKEATVKVAIEKAG
                     INPIIETVTANGKIQPETEIKISPDVSGEIVQLDVREGDFVQKGTLLFKIKPEIYISA
                     RDRAAATLNSTKARLAQVEAQMIQAELAFNRSKKLFEENTISQADFEQAEAQYKVAKA
                     EMESARYSVKSSEASLKEANENLIKTTVYSPMTGTISSLLVEQGERVVGANMMTGTEV
                     LRVADMNRMEVVVDVNENDIIRVKLGDTAIVEVDAYIDRDFKGIVTEIANSANTLGTT
                     SDQVTNFEVRILILKESYDDLISENNPSPFRPGMSASVDIYTNRKAGAMTVPIQAVTT
                     RTDTTITDPAARNDIRTLVFVSDGTYALARDVKTGIQDNVNIEILSGLTGDEDVIVQP
                     FSAISKKLSDSTRIEVVEKETLFSTEKSKK"
     gene            complement(7614..8981)
                     /locus_tag="PLA17_03695"
     CDS             complement(7614..8981)
                     /locus_tag="PLA17_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK84548.1"
                     /translation="MKRKTLYLSFILLLGPLALFSQQKKWTLEECIAHALQNNISIKQ
                     QEIMNRYQVAALEQSKLNLLPTLNGSASHNYAFGRALDETTYEFTENETVQSNNFYAG
                     SNVTLFRGLVNYNTIRKNMYEVLASEQELEQFRDNISLNVSLAYLQILLNQELVTATE
                     SQVELTKQQIDRTRKLVEAGSLAQGSLLEIEAQAAREELQLVNMRNQLALSLLTLAQM
                     LELQNIDDFEVAIPVITVRDEAVDGNPAAIYSIAEKMRPEIMSAEYKLKSAEYDLAIA
                     KGNRSPRLSLGASVSTGYSDMRLKPITNENYPFREQLNDNLNYGLGLSLNIPIFNGWQ
                     VNTLIKNSMLGIENSKYALENSKKQLYKNIQQAYTDASGALKKYKASQKAVISMEEAF
                     RYAEQKLGVGLVTAVEYNQSKTQLLNAQSEMAQAKYEYVFKTKVLDFYKGVPLTLEHI
                     TIFAK"
     gene            9148..10224
                     /gene="fbaA"
                     /locus_tag="PLA17_03700"
     CDS             9148..10224
                     /gene="fbaA"
                     /locus_tag="PLA17_03700"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPK84549.1"
                     /translation="MTNKGLNLEPGVISGEDVKKLFDHANKNDFAIPAVNVVGTDSVN
                     AVLETAREVNSPVIIQFSSGGAHFFGGQGLAKENHMGAIAGGISGALHVHNVVEYYGI
                     PVILHTDHAARKLLPWIDALLDAGEEFYELNGKPLFSSHMLDLSEEPLKENIETCKRY
                     LERMNKMGMTLEIELGITGGEEDGVDNTGIESSRLYTQPEDVAYAYEELMKVSHNFTI
                     AASFGNVHGVYKPGNVTLRPEILKNSQDYIRKKFKTGPNPVNFVFHGGSGSEKHLIAE
                     AIKYGAIKMNIDTDMQWAFWAGVRDYYEGKKDYMQGQIGNPEGEDKPNKKYYDPRVWL
                     RKGQENFIARLKEAFADLNAVNRL"
     gene            10360..10602
                     /locus_tag="PLA17_03705"
     CDS             10360..10602
                     /locus_tag="PLA17_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84550.1"
                     /translation="MRRGSFTKEKIKRTTTFTLRLNEREAKALNIYCKRYRVSSKSDF
                     MRRTIMQAIVVRFDADHPSLWEQGELNLFSHEAKNN"
     gene            10680..11063
                     /locus_tag="PLA17_03710"
     CDS             10680..11063
                     /locus_tag="PLA17_03710"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPK84551.1"
                     /translation="MELEIIKIEPTRVTPRVLFLEGHIELRGRSISENSTDFYKPLAA
                     WVDSYVEKTEVRTRVIFAFDFINTASTKWIYSIVKRLALYPNVRELVSIDWYYEKGDD
                     DQYELGQIIHSFIDCPFIFYETEQT"
     gene            11111..11183
                     /locus_tag="PLA17_03715"
     tRNA            11111..11183
                     /locus_tag="PLA17_03715"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11144..11146,aa:Gln,seq:ttg)
     gene            complement(11240..11383)
                     /locus_tag="PLA17_03720"
     CDS             complement(11240..11383)
                     /locus_tag="PLA17_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84552.1"
                     /translation="MDERPEVRRIGAPEGWSTGGTEDQKSGEMEHRSAGGPFACTGCL
                     FMA"
     gene            complement(11424..11963)
                     /locus_tag="PLA17_03725"
     CDS             complement(11424..11963)
                     /locus_tag="PLA17_03725"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPK84553.1"
                     /translation="MSSKPLRFTDGVVTIRKLRRSDKFRLAEIANNEKIAANLRDSFP
                     SPYTLEDAQKFISMCLRQVPYEIFAIEFEGEFVGTIGLHRQSDVYRKTAELGYLIGEP
                     YWHKGIAPRAVNLICEYGFREMDIIKIFSAVFAFNTASQRVLEKCGFTLEAVLKAAAI
                     KNGRICDEYRYALIPEKYK"
     gene            complement(11996..12142)
                     /locus_tag="PLA17_03730"
     CDS             complement(11996..12142)
                     /locus_tag="PLA17_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84554.1"
                     /translation="MANRFFFLWLPETTLITGIIAGRHLLNTRMAAFRNGTMSLFNIQ
                     SCTI"
     gene            complement(12201..13472)
                     /locus_tag="PLA17_03735"
     CDS             complement(12201..13472)
                     /locus_tag="PLA17_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84555.1"
                     /translation="MKKTLDINIAGQLFRVDEDAWEVLKHYLDHVSARFKSEQGADET
                     LADIEARIAEIFGGGKEPPTLVSKEMVTNMINIMGAPEDYYDDGPAVKYKKLYVRKSM
                     YDPNSFSARLGRTLSGFFTAFGKLMSAIMRVFAIILGAFFTLFGFLLFFTFVILIFFN
                     NAPFFASVMEPQITNVHGLLSIVLNTNAIWPILILAALVTLLPLAAVIWLGIKLIFRI
                     RESFRVLNIVLFLVWTASLCALAVILSLQLSVYSNRESVEKRLTLDPAPKTLWINTMK
                     KQANLSHDRYASVEDFRFFVDRSNDILHASPELRIRGSDNGTGYIAVQRRANSNSDTE
                     AVRNARQIEYDWKMSGDTLYLDEYCTLPPGAKWNGSIVDIDIRLPEGTEIRFVPEVSP
                     DVLNFHLFPWKDQAWKIVDGWPRSIDDRTDQ"
     gene            complement(13481..13810)
                     /locus_tag="PLA17_03740"
     CDS             complement(13481..13810)
                     /locus_tag="PLA17_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPK84556.1"
                     /translation="MNTDNIRAQMRKGILEYCILAVLSRNSCYAVDIINELKKARVIV
                     VEGTLYPLLTRQKNAGLLSYRWQESPQGPPRKYYELTELGQIYLRELDKVWDELVESV
                     NQIRDRK"
     gene            complement(13853..14428)
                     /locus_tag="PLA17_03745"
     CDS             complement(13853..14428)
                     /locus_tag="PLA17_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPK84557.1"
                     /translation="MTVNINLGGYAFYLDDDAYERLRQYIKSLEKEFSGEPGAAEIMS
                     DIEGRISELFRGKLSSYKQVITLKDVEEVMAILGSPEDISGVDSAGEVPPRSQRRMYR
                     DPDRRIIGGVCAGISAYLDWQSYIMRIIFTALIFAGGFSLLLYLILWIVLPEAKTTAQ
                     KLEMRGDPVTIENITDSVRQEFNTVKEKMNL"
     gene            14593..15606
                     /locus_tag="PLA17_03750"
     CDS             14593..15606
                     /locus_tag="PLA17_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK84558.1"
                     /translation="MTNFIEKSKSMKVYNWGILGTGNIAHKFARALMLLANANLYAVG
                     SRDAGRAEKFAGEFGFSRHFGSYEEMLADPGIDVVYISSPHTMHMEHTLMALRNGKHV
                     ICEKPMSITAEEVSLMADEAKRNDLFLMEALWPPFQPSYLKADEILRSGKMGKLLHIR
                     GKFAFAVPYDPELRTYNLALGGGALLDIGIYPVMDILRYLGEPENIVATSVLAPTGAD
                     QSTAMIFKFSDGRMADAYCSFTNMGGVSTEFYCEKGNLILTRGRDRNQHLIIEVQGDV
                     DDRIFTPPAQGYQYEAAEVMNCLDRGLTESPVVPLSFSLLLASILDEVRRKAGIIYPG
                     RDR"
     gene            15628..16386
                     /locus_tag="PLA17_03755"
     CDS             15628..16386
                     /locus_tag="PLA17_03755"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPK84559.1"
                     /translation="MTKDSSAGERKRLLLSMLIPFMLVFLMWLMRGASLLFGNEFYFL
                     GIYPGRVSSLTGIITSPFVHSGIRHLVNNSLPLFILGTALFYFYSQVSFRVLLWITLL
                     TGLAVWITGRPAWHIGASGIVYGLAAFLFVSGIIRRHIPLMGLSLLVVFLYGSMVWGI
                     FPGFGMDISWESHMLGAGAGVILAIWYRHEGPQKPVPFYEREEENETGEDSGEVAAVD
                     PVTGAGSAAVTDSVTEAGFKTITRAGPAPESRSK"
BASE COUNT         3855 a         4055 c         4193 g         4423 t
ORIGIN      
        1 tgtatggctt gacccggcaa aaaccacacc ttatcagttc taccagttct ggctcaatgt
       61 ggctgacgcc gacgcggaaa aattcatcag gatattcaca atgttaccac agtcagaaat
      121 aaccggactg gcagaggctc accaggctga gccggaacgg cgtatactgc aaaaacgcct
      181 tgccgaagag gtgactgtaa tgactcactc cagggaggag tacgaggccg cggtggaagc
      241 ctcacagatt ctcttcggga aagggaccac cgaacagcta agaaagatga atgaaaacac
      301 ctttcttgca ctgtttgagg gagtgccgat gtttgatatc agcgcaggcc tgctggaagc
      361 aggggtgact ttagtcgacc tctgtgcagt acataccgcc atcaccgaat cgaaaggtga
      421 gttccgccgc cttatcagcg gagggggtgt gagcctcaac aagagtaaga tggacaatgc
      481 ggagatgatt attacacggg aacatcttct gaacaacaag tatatcctca tacagaaagg
      541 gaaaaagagt tacttcctta tcagggtaaa ggaggagtga ttgactgaac ataattcaaa
      601 tcaaacaggg aggtcagtat gaagggcaac atcagggcag aattcaggca ggactcattt
      661 gacctgttga actttttcat caggcatttc aggaagtttg tgatagccgg catcgttgcg
      721 gccattctgt cagctgttat atcactactg atcagaccat tgtacgaatc aacggtcatt
      781 ctctatccct cttcaaacat taccgaagcc acctcactgc ttggcgagac tgaatcgcgc
      841 actgccctgt tcggtgacga tgacgccacc gagaaactca tccaggtgat caacagcgcg
      901 caggtcaggg agtatctgaa ggagaagtat aacctgatgc agcattataa tatcaagccg
      961 ggcgaaaaat acccgaatac cctcatgaag gcaaagatgg acaagtacat gcgttgtacc
     1021 cgtacagatt acggatcagt gaagatacgc gtccgcgacc gcgaccgggt gtatgcctgt
     1081 aacatggcaa acgatatggc cgccagggct gacaccatct tcaataacct gcagcgcgaa
     1141 gctgcaatgg tgacgctgga tgagataagc cgcctctatg atatccagaa acgcctggtg
     1201 aggcagtacg aagacagcct tatgaacctc agcagcgctg ctgccctgag gctctactcc
     1261 acctttgaga tggagaatga ttaccttggt ctgatgcgcg gccgtttcct ggaggcccag
     1321 gcactgagcc gccagactct tccctatatc cttgttgttg acagggctgt ggtggcagag
     1381 aagaaggtgt ttccgaaacg aactgtcatg gtgttgctgt caacagtctc agtgttgctg
     1441 ctgctggcca tggttctgtt catagccgag ggggtgaagc tccggcgtgc cgatgacagg
     1501 caatgaactg ctgatactga tcatcccggc agtgtttgcc atactgctgc tggcgctact
     1561 ctcacttgag tatctccttc ttcttacgct tttcctgaca ccgctctccc ttcagctgag
     1621 ctatctgacc ggtagtaccg gctttgatct gtcactcccc acagagccgg tcatggcctt
     1681 tctgctgctt acagtcctgt tccggcttac cgttacacgt gaatttccga aggagctgct
     1741 gaggcatccg atgacgtgga tggcaggcct gtacctgttg tggacattca taacagccct
     1801 gacatcgacg ataccgggag tttcattcaa gacgctggca taccggctgt ggttcatggc
     1861 aggcttttac cttgtgacag cccggctggc atttgttgag cggttcagaa aaagatacat
     1921 actggcttac gcagccggac tggcagtggt ggttatctac ttcatcatca gggtacaggg
     1981 agcaggcctg ctcaaccagc agtttgccca ttcggcctgt tatccgtttt ataaagacca
     2041 cacctcattt ggcgcctcga tggctttcat cattcctccg ctgacagtga tgctcttctc
     2101 caggggcagc ctgtggcaca ggggttttct ggcagccctg ctgctgatct tcgttgcagg
     2161 ctttgtcctc tcatacagcc gtgccgcctg ggtgagcctg atagctgcat cggccctgtc
     2221 gctgatcctg tggaccagaa tgcctgtgaa gttactgtca ctatccgcac tccttttcgt
     2281 tattgttctg gccttctctg ccggttctat ctggcagaag atggacagca ccactgaaga
     2341 ctcatccact gacctgggcc agcatctccg gtcgtcatca aatattacca ccgaccagtc
     2401 gaaccttgaa cgtatcaaca gatggaaatg tgcgctgagg atgttcgctg agaaacccaa
     2461 tacgggctgg ggtccgggaa cttaccagtt ctgttatgcc ccttttcaga aaccgtctga
     2521 gcggacggca ataagcactg attttggcga tgccggtaat tcccacagtg aatatctcgg
     2581 tgcattgagc gaatcaggct ggccgggggc gctgatattc ctggctatga ccctttgcgc
     2641 tgtcattacc ggaataaagg tgtggtaccg cgaagggaga ggggaaaagg gttattttgc
     2701 cctggctgtg gtgacggggc tggtgaccta catggttcac ggggttatga acagcttcct
     2761 tgacagtgat aagatagcag ccctgtggtg ggggttcatt gcactgattg tggcgatgga
     2821 cctcgatggg cagagaattg ccggcgctcc ggaaagtgac agcagtccgg cgtagctttt
     2881 caccctgatg tccggagtag ctttctgctc tgatgtccgg tccagctttt cgccctgata
     2941 accggcatag ttttctgctc tgatccccgg caggatgaag agtaagaaag aatcagggaa
     3001 actctatcgc aaggattgag acatcatcga agagtcgctg gttgttgctg cttatgtcta
     3061 tatcactgat aagagattca atattatctg ccagcggctt cctgttgggg aaatgcttca
     3121 ccaggggagc tattccgtag tccctgcctc cttcatcctt aagttctgag aggccatcgg
     3181 tgtaacagat gattttggtg ggattcttaa gtgtcagggt ttccgttttc acacagggga
     3241 tatcatctat catgcccact cccacgcatg taggtcttag aagcgatgac tgcccggtgc
     3301 tgagattgat aataagcggc ggggtgtgag cagcattgac atactccagt tcgcgtgatc
     3361 tggtattgta ccgggcaata aacagtgtta tgaacccttc tccgtccgag ttggtgacca
     3421 caagggtgtt cagtgtcctg ataagttcct cgaagcttgc atttgcggta aagagagccc
     3481 ggaggcttgc ctggaaattt gacatcagta tggctgcaga catccccttc cctgagatgt
     3541 cggcaataca gaaccctgca atctcctctg acagctgcat gaaatcgtag taatcaccac
     3601 ctacctcata atgggggaaa taaaaacctt tggccctgat gagattgtct gaagggagct
     3661 tggtgttgtt agggataagc ttcatttgca tccgggcagc aagctcaagt tccctgcgaa
     3721 gggcctcctg ccggaggctc tcattgaaga gcctctggtt ctcaatggca acaacgatga
     3781 tgccggaaat tgtctgaatg aagtggaggt gctttataag cggactcatt ccttctctct
     3841 cctcgtcgat ctcaccaatc aggacataag ccgagggttc gttgttgtga agaacgggga
     3901 ttataaagtc aacggccctg aagcgctcct gtggaacctc ggtctgtatc tccctgaaat
     3961 ggagcaggtc attttcaacg tcgacagact ctttcagtga ttcggggaat cctgaactga
     4021 gcaggggctc ccactgttcg ccatatttga aaagcagaat ctttccaata cccagttcat
     4081 cacggaggat tgactcatat ttcccgagca acgttgttgt aggaaggttg gcattgatgg
     4141 agagggtaat gtcaagcagg gcatcgagct tgaacttcga catcttcagc ctgtctttcg
     4201 gtggcctgcc ttctctcgtc ttcacgtcag ccctttaccc tttctatgta tgagcggtca
     4261 cgcgtgtcaa tacgtatctt gtcaccggca ttgacaaata gtggtaccat gattgttgat
     4321 ccggtctcga ttgtagccgg cttaagggtg ttggttgccg tatcaccgcg gaggcccggc
     4381 tctgaatagg ttacttccat taccacgctg accggaaggt ctaccaggag cacattctcc
     4441 gtatcagcat gcaccactac ctctaccgta tcaccctctt tcagaagatc actgttttcg
     4501 atcatcgaat ggggtactgt tatctgatca aatgtctcaa cgtgcatcag gtgatagccc
     4561 atatcatcac tgtaaaggaa ctggtggggt ctgcgttcca cccttgccac attgaccttc
     4621 actccggagc tgaatgtatt atcaagtacc tttccggttg taacgttctt gagtttcgtt
     4681 cttacgaagg ccgggccctt tcccggtttt acatgctgaa actgaacgat ggtataaagg
     4741 tcatcattga attctatcac catcccgttt ctgaaatctg ctgttgtagc cataaagaaa
     4801 aacaattcat tttggttagt caataaagct gaggggcgct ctaagccctt catagcttac
     4861 catatccacg taaaatgacc ctacaaagat atcaaatcgt attttaaccg gtaaaaaatt
     4921 ttcatctgta gtgaaccaca ttgccatatc ctcctccgtt ttgaatactc tgccaacctc
     4981 ggtcacggga tggaaacggt agcattcgat ccgcttcccc cttaccctga cagtctcttt
     5041 ccccttgtat ctcaatataa taggatacaa ttcatcggcg aaccatgtca tcattttgta
     5101 tgtctctccc gggacaagct ctttcccgga agccaggtaa tatttcctga accagtaaaa
     5161 gcaggagaca atgtcaagga gccccttcgg tcctttatgg ttacctgtga ggtcactgaa
     5221 gacgagggtt gagtcagttt ctgacatgtg gtcaaatcca acctcgttgt atttgcggta
     5281 gcgtccctcc tggatattcc ttatgctgaa aaccggaagg tcattttccg ggtcaatgta
     5341 gctttcatag acatctctca ccctgtagag ggcgtcggcc aggccggtgg tcttcccgga
     5401 tatcactgca tgccataccg gctgcccctt gtggaacccc tcactgagct cgaggcttgc
     5461 ctgaccgctg cccacgatac catatttaat ctggtatgtt accttttctc cggggtggta
     5521 gggctgcttc tgggcatcaa gcaattgcag ggctgcagtt attgctatca gggccgcaat
     5581 taatattttt ttcatttctc attcaggttt gtccgggaag aggcttttcg ggacggtggc
     5641 aatgaactcc ttgaggtaat agggttcgaa gtatgccagg tcctcaaatt gcctgttcct
     5701 cacagcattt actgacggga ttagcaaata tgatgccgag aggctgaagt catccctgaa
     5761 caaggcattc gggccggtaa taatgccgcg gcatttgccg gacccgttcc cgaagaaaat
     5821 cacccggttt tcagagagga attcactgaa tgagtctttg tcgattatgc ttgccgaagt
     5881 ttccatgacc tgtgttccgt cagcggcaaa aagggcattg tagacttcca tgcggcgggc
     5941 atcgatcatc gggcacagga gggtcccggg cagaaggtct tccgggtgga ggcttatgta
     6001 gccgctgcac attgcagcca tggtgtctat tcctataagt ggtatacccg cgccgtaagc
     6061 tatgcctttg gcgactgaga cacctattct cagtcccgtg tatgaacccg gtcccttgct
     6121 gacggcaacc gcatcaaggt ctgttgccgt gatgcggttt tctttcatga gttgttgaat
     6181 gattactgtg agttgtgagg catgagacat gccttgcggt gcctcaatga ggccatgcac
     6241 cctcgtctca tcacacaagg cggcagagca tacactggtg gctgtctcaa tgcagagaat
     6301 cattcagtta tgtaagtaaa ttctttactt ctttgacttt tcagtgctga agagagtctc
     6361 tttctctaca acctctatgc gtgttgaatc agacagtttc ttggagatgg cgctgaaagg
     6421 ctgaactatc acatcctcat ccccggtgag tccgctcagg atctctatgt tgacattgtc
     6481 ctgtatgccg gtcttcacgt cgcgcgccag cgcataggtt ccgtctgaga caaacaccag
     6541 ggttcgtatg tcattcctgg cagcagggtc ggtaatggtt gtgtcagtgc gggtggtaac
     6601 ggcctggatg ggcacagtca tcgcaccggc tttgcggttg gtgtatatgt caaccgaggc
     6661 agacataccg ggcctgaacg ggctgggatt gttttcagag atcaggtcat catatgattc
     6721 tttaagaatg agtatcctga cctcgaagtt ggtcacctgg tcagaggtgg tcccgagggt
     6781 gtttgccgag ttggctatct cagtcacaat acccttgaaa tcacggtcaa tataggcatc
     6841 cacctcaaca attgcggtat cgccaagttt aacccttatg atgtcatttt cattgacgtc
     6901 aaccaccact tccatccggt tcatgtctgc cacgcggagc acctcggtac cggtcatcat
     6961 gttggcaccc acaacccttt cgccctgttc aaccagcagg cttgagatgg tgcctgtcat
     7021 gggtgagtag acagtagttt ttatcagatt ctcatttgcc tccttgagtg atgcctcaga
     7081 gctcttgaca gagtaccggg cgctctccat ttcggctttg gccaccttgt attgtgcctc
     7141 cgcctgctcg aagtcggcct gtgagattgt gttttcctca aacagcttct tgctcctgtt
     7201 gaaagccagc tcagcctgga tcatctgggc ctccacctgt gccaggcggg cctttgtcga
     7261 gttgagtgtg gcagcagcgc ggtcacgtgc tgagatatag atctccggct ttatcttgaa
     7321 aagcagggtt cccttctgga cgaaatcgcc ctccctgaca tcaagctgga cgatctcgcc
     7381 ggagacatcg gggctgatct ttatctcggt ttcaggctgt attttgccgt ttgcagtcac
     7441 cgtctcaatg attggattaa tacctgcctt ttcaatggca accttgacgg tcgcctcttt
     7501 accaaaccag ccggccttct tgcccacgac ggcaagaatg aggaggagaa tgacggcaag
     7561 aataagggtt ttaagaagtc tgttattatt catcggttga atttttactg gaattatttg
     7621 gcaaagattg taatgtgttc aagggtcagc gggacacctt tatagaaatc gaggaccttg
     7681 gttttgaaga cgtactcgta ttttgcctgg gccatttcgc tctgggcgtt aaggagttgg
     7741 gtctttgact ggttatactc cacggcggtg accaatccca ctcccagttt ctgttcggca
     7801 taacggaatg cctcctccat ggagatgaca gccttctggc ttgctttgta cttcttgagt
     7861 gctccgctgg catcagtgta tgcctgctgg atattcttat aaagctgctt tttgctgttt
     7921 tcgagggcat actttgaatt ttcgattccc agcatggagt tttttattag ggtgttgacc
     7981 tgccagccgt tgaagatggg gatattaagt gataatccga gtccgtaatt caggttgtca
     8041 ttcagctgct ccctgaacgg gtaattctca ttggttatgg gcttgagcct catatctgaa
     8101 taacccgttg acactgaggc gccgagactc agccgcgggc tcctgttgcc tttggctatt
     8161 gcaaggtcat attcagcact tttgagtttg tattcagcgc tcattatctc cgggcgcatc
     8221 ttttctgcta tcgaatagat tgctgcagga ttaccgtcaa cagcctcgtc cctcaccgtt
     8281 atgacgggga tggctacttc aaaatcgtca atattctgca gctcaagcat ctgggccagg
     8341 gttagaagtg acagtgcaag ctggttacgc atgttcacca gttgcagctc ctcgcgtgcg
     8401 gcctgtgcct ctatctcaag caggctgcct tgtgccaggc tgccggcctc taccagcttg
     8461 cgggtccggt ctatctgctg ttttgtgagt tccacctgtg attctgtggc tgtcacaagc
     8521 tcctggttga ggagtatctg aagataggcc agggatacat tcagcgagat attgtctctg
     8581 aactgctcaa gctcctgttc actcgccagc acctcataca tgttcttccg gattgtgttg
     8641 tagttcacca gccccctgaa aagggtaaca ttgcttccgg cgtagaagtt gttcgactgt
     8701 actgtttcgt tctcggtgaa ctcgtatgtg gtctcgtcaa gtgcacgtcc gaatgcataa
     8761 ttgtgagatg cgctgccatt gagggtagga agcaggttaa gctttgactg ttcaagcgct
     8821 gcaacctgat acctgttcat tatctcctgc tgctttatgc tgatattgtt ctgaagggca
     8881 tgcgcaatgc actcctccag tgtccatttc ttttgctggg agaatagcgc aagcggaccc
     8941 agcaaaagaa taaagctcag gtaaagagtc tttcttttca taatctgatt gacagcatta
     9001 attaagtgta cgaagatatg aaatagatca atattttaaa gacagcaaag atattatgat
     9061 gttgcataca gcctgtcaac agtatcattc atttttgtgt atctttgcgc agcctgacta
     9121 caaaaaaaca atcaaattta taaaacaatg actaacaaag ggttaaatct ggagccgggc
     9181 gtgatcagcg gtgaagatgt gaagaagctg tttgatcatg ccaataaaaa tgatttcgcc
     9241 attcctgccg ttaatgtggt aggaaccgat tctgtcaatg ctgtacttga gacagcccgt
     9301 gaggtaaatt caccagttat catacagttt tcaagtggag gtgcacattt ctttggaggt
     9361 cagggacttg ccaaggagaa tcatatgggt gccatcgccg gaggcatatc gggtgcattg
     9421 cacgttcaca atgtggtgga gtattatggc atacccgtaa tactgcatac tgaccatgct
     9481 gcccgtaagc tcctgccctg gattgatgcg ctgcttgatg caggcgagga gttttatgag
     9541 ttgaacggca agccgctgtt cagctctcat atgcttgatc tctcggagga gccgctgaaa
     9601 gaaaacattg agacatgcaa gaggtatctt gagaggatga acaagatggg gatgaccctt
     9661 gagattgaac tcggcatcac cggtggcgag gaggacggtg ttgacaatac cggcattgaa
     9721 agctcgaggc tttacaccca gcctgaggat gtggcatatg catatgagga actgatgaag
     9781 gtttcacaca actttacaat tgctgcatca ttcggtaacg tacacggtgt gtacaagccc
     9841 ggtaacgtaa cgctcaggcc cgagatactg aagaactcgc aggactatat ccggaagaaa
     9901 ttcaagaccg gccccaatcc cgtgaatttc gtctttcacg gcggctcagg atcagaaaag
     9961 cacctgatag ccgaggccat taagtacggc gccatcaaga tgaacattga cactgacatg
    10021 cagtgggcgt tctgggcagg tgtcagggat tactacgagg gcaaaaagga ttacatgcag
    10081 ggccagatag gcaatcctga gggagaagac aagcctaaca agaagtatta tgatccgagg
    10141 gtatggcttc gcaaaggtca ggagaacttc attgccaggc ttaaggaagc gtttgccgac
    10201 cttaatgcag tgaacaggct gtaagtttta ttaataagct aacataaagc agatcagctc
    10261 attgcagttg atctgctttt gtattgttga taagttgtat tattcatttg ctcactggat
    10321 ggccatgcgg gctactttag cacaaaattg acttcctgat tgagacgcgg atcttttacc
    10381 aaagaaaaga ttaagaggac caccacgttt accctcaggc ttaatgagag ggaggcgaag
    10441 gcgctcaata tctactgcaa gcggtacagg gtaagtagca agtcggattt catgaggcgg
    10501 accatcatgc aggccattgt cgtacgtttt gatgcggacc atccttcatt gtgggagcag
    10561 ggtgagctga acctgttctc tcatgaggcg aaaaataact gaaaagagtc agggcaactc
    10621 ttattttatt atttttgtta ctccacgata gcggttgtaa ggtatcgact cggaagaaaa
    10681 tggaattgga aataattaaa atagaaccca ccagggttac cccgcgggtt ttgttcctcg
    10741 agggtcatat tgagctgagg ggcaggtcaa tatctgaaaa ttcaactgat ttttacaagc
    10801 cgttggcggc atgggttgac tcctatgttg agaagactga ggttcgcacg agggttattt
    10861 ttgcctttga tttcataaac acggcctcaa cgaagtggat ctattccatt gtcaagcggc
    10921 ttgcccttta cccgaatgtc cgcgagttgg tttccattga ctggtattat gagaagggtg
    10981 atgatgatca gtatgagctg gggcagataa ttcactcttt tatcgactgt ccgttcatct
    11041 tttacgagac tgagcagacc tgaaaatcag gaaaaatatt tatgtttgca gcgccttgtc
    11101 aggcgccgat tgacccatgg tgtaattggc aacacaacgg attttggttc cgcagagtcc
    11161 tggttcgagt ccaggtgggt caacagagaa cagccggaag gctgtttttt gttgaagatc
    11221 ggcgaattcg ccggcataat tatgccataa acaggcaacc ggtacaggca aatggccctc
    11281 cggcactacg gtgctccatc tctccggact tctggtcttc tgttcctccg gtgctccatc
    11341 cctccggtgc tccaatcctc cggacctccg gcctttcgtc catccggcct tcctgtcctt
    11401 tggtcctccg gtgcgcactc tgatcattta tatttttcgg gtatcagcgc ataccggtac
    11461 tcatcgcata ttctcccgtt ctttatcgcg gcagccttca gcaccgcttc cagggtgaag
    11521 ccgcatttct ccagcacacg ctgtgaagca gtgttgaatg caaacaccgc tgaaaatatc
    11581 ttgatgatat ccatttcgcg aaagccatat tcgcagatga ggttcactgc ccggggagct
    11641 attcccttgt gccagtaagg ttcccctatt agatagccaa gctcggccgt cttgcggtat
    11701 acgtcactct gcctgtgaag gccgatggtt cccacaaatt ctccctcaaa ttcaatggca
    11761 aatatctcgt atggcacctg cctgagacac atcgagatga acttctgggc atcttccagg
    11821 gtatagggcg aagggaatga atccctcagg ttggcggcta ttttttcatt gttggctatc
    11881 tctgccaggc ggaatttgtc actgcgtctg agcttgcgga ttgtgaccac accatcggta
    11941 aatctcagtg gttttgaact catggctgta tgaccgggtt aggcccgtaa aggtattaaa
    12001 ttgtgcagga ttgtatgttg aaaaggctca tggtaccgtt ccggaaggcc gccattcttg
    12061 tattcagaag atggcgtccg gctatgatgc cggttatcag ggttgtttca ggcagccaaa
    12121 ggaagaaaaa gcggtttgcc ataaaaaatc ccggttgtta aggccgggat attgaagggt
    12181 aaaaatacgc aggtacagat ttattgatct gtccggtcgt ctattgagcg cggccaacca
    12241 tccacgattt tccatgcctg gtctttccat ggaaacagat gaaagttcag aacgtcgggt
    12301 gaaacctccg gcacgaacct gatctcagta ccctcgggaa gccgtatgtc tatatccact
    12361 atcgatccgt tccactttgc tcccggcgga agggtacagt attcgtcgag gtacagggtg
    12421 tcacctgaca ttttccagtc atattcgatc tgccgtgcat ttctgacagc ctcagtgtcg
    12481 gaattgctgt tggcccttct ctgcacggcg atgtacccgg tgccgttatc actgccgcgg
    12541 attctcagct ccggcgatgc gtgaagaata tcattgctcc tgtccacgaa gaatctgaaa
    12601 tcttccaccg aagcgtacct gtcatgactc aggtttgcct gcttcttcat cgtgtttatc
    12661 cagagtgtct tcggggcggg gtcaagcgtc agcctctttt ccactgactc cctgtttgaa
    12721 tagactgaaa gctgaaggct gagtataact gccagtgcac acaacgaagc agtccatacc
    12781 aggaagagca ctatattcaa caccctgaaa ctctccctga tcctgaatat aagcttgatg
    12841 cccagccata tgacagcagc cagcggcaag agtgtcacca gcgccgcgag aatgagtatc
    12901 ggccatatgg cgtttgtgtt gagcactatg ctaagaaggc cgtgaacatt tgtaatttgc
    12961 ggctccatca cggaggcaaa gaatggggca ttgttgaaga agatcagaat cacaaaggtg
    13021 aagaagagca gaaatccaaa gagggtgaaa aatgccccca gaattattgc aaataccctc
    13081 atgattgccg acatcagctt cccgaatgcc gtgaagaatc ctgacagtgt cctgcccagc
    13141 ctggctgaga aactgttggg atcatacatt gatttcctta cataaagctt cttgtacttt
    13201 accgccgggc cgtcatcata atagtcctca ggcgcaccca taatattgat catattggtg
    13261 accatctcct tcgagacaag agtgggaggc tccttgccgc ctccgaagat ctccgcaatg
    13321 cgcgcctcta tgtcagcgag cgtctcatcg gctccctgct cggatttgaa cctggcagag
    13381 acatggtcaa gatagtgttt cagcacctcc caggcatcct cgtccacacg gaagagctgc
    13441 ccggcaatgt tgatgtcaag ggtttttttc atggctgcct ttatttcctg tcacgtatct
    13501 ggttgaccga ttccacaagc tcgtcccata ctttgtcaag ttccctgaga taaatctgtc
    13561 ccagctcggt aagctcgtaa tatttcctgg gtggaccctg cggcgactcc tgccagcggt
    13621 agctgaggag ccctgcgttc ttctgccttg tcagcagggg gtagagtgtt ccttcaacga
    13681 ctatcacccg ggctttcttg agctcattga tgatgtcaac ggcgtagcag gaattccttg
    13741 acagcactgc caggatgcag tactcgagaa tccccttgcg catttgtgct cttatgttgt
    13801 ccgtattcat ggcttgttct ttattgtctg ctttttatgc cttcggcgaa ggtcagagat
    13861 tcatcttttc ctttacggtg ttgaactcct gcctcacaga atccgttatg ttttcaatgg
    13921 tgaccggatc accccgcatc tccagcttct gtgcggtggt ttttgcctcc ggaagcacta
    13981 tccacagaat gagatagagc aacaggctga agccacctgc aaatatcagc gccgtaaaga
    14041 tgatcctcat tatatacgat tgccagtcga ggtatgccga gattccggca catacccctc
    14101 ctatgatccg cctgtcagga tcacggtaca tcctgcgttg tgacctgggc ggcacttcgc
    14161 cggcagaatc aaccccgctt atatcttcag gagatccaag tatggccatc acctcctcca
    14221 cgtccttcaa ggtaatgacc tgcttgtagc tgctgagctt tcccctgaac agctccgata
    14281 tccttccctc gatatcggac attatctcag cagcccccgg ctcacccgaa aattcctttt
    14341 ccaggctctt tatgtactgc cggagccgct cgtaggcgtc atcgtcgagg tagaatgcgt
    14401 accccccgag attgatgttg acagtcattt tcatcgtttc tattataagg tacctgttaa
    14461 tacaaattaa tattgcattg caaatatatg aatttaaaat aatactatgc aatacataga
    14521 agtaaaaatt taagatattt tttgaaggga tttcagactt atcacccggg caaatggcaa
    14581 aataggggat gaatgactaa ctttatagaa aaaagcaaga gtatgaaagt gtacaactgg
    14641 ggaatcctgg gcaccggcaa cattgcccac aaatttgcga gggcgctgat gctgcttgcc
    14701 aacgctaatc tttatgctgt tggttcgcgt gatgccggca gggcagagaa gtttgccggt
    14761 gagtttgggt tcagcagaca tttcgggagt tatgaagaga tgctggccga tcccggcatt
    14821 gatgtggttt acatctcctc gccgcatacc atgcatatgg agcatacgct catggctctc
    14881 cgcaacggca agcatgtcat ctgtgaaaag ccgatgtcaa taacggcaga agaggtatca
    14941 ctaatggctg atgaagcgaa gaggaatgat cttttcctga tggaggcatt atggcctccg
    15001 ttccagccat cgtacctgaa ggcagatgag atactgcgta gcgggaagat gggaaagctg
    15061 ttgcatatcc ggggtaagtt tgcctttgct gttccgtatg atccggaact gcgcacctac
    15121 aatctggcac ttggcggtgg tgcactgctt gacataggca tctacccggt gatggatatc
    15181 ctgagatatc tgggtgagcc tgagaacata gttgctactt ctgtccttgc ccccaccggc
    15241 gctgaccaga gtactgccat gatcttcaaa ttcagtgatg gccgcatggc tgatgcttac
    15301 tgctcattta caaacatggg gggtgtttca acagaattct actgtgagaa gggcaatctc
    15361 atccttaccc ggggacgtga ccggaaccag catctgataa tagaggtgca gggtgatgtt
    15421 gatgaccgta ttttcacccc tcccgcccag ggatatcagt acgaggctgc cgaagtgatg
    15481 aactgtcttg acaggggtct gaccgaaagc cccgtcgtcc ccctctcctt tagcctcctg
    15541 ctggccagca tccttgatga ggtgcgccgg aaggcaggca taatatatcc gggacgtgac
    15601 cgttagtgca accatctgct gaacggtatg acgaaagact catctgccgg cgaaaggaag
    15661 cgcctgctgc tcagcatgct cattcctttc atgctggtct tcctcatgtg gctgatgagg
    15721 ggggcgtcac tgctgttcgg caacgagttt tattttcttg gcatctatcc cggcagggtc
    15781 tcttctctca ccggcatcat cacctctccg tttgttcatt ccggaatcag gcatctggtc
    15841 aataactccc ttcctctttt tatccttggc acggcgctgt tttacttcta ctcgcaggtc
    15901 tcattcaggg tactgctgtg gatcacgctt ctcaccggcc ttgccgtgtg gattacaggc
    15961 cgtccggcct ggcatatcgg ggcaagcggc atcgtttacg gtctggctgc gtttctattt
    16021 gtcagtggta ttatccgcag acatatcccg ctgatgggtc tgtcactcct tgttgtcttt
    16081 ctctacggca gcatggtctg gggaatattt cccggatttg ggatggatat ttcatgggaa
    16141 tcgcatatgc tcggtgcggg agccggagtg atacttgcaa tatggtatcg tcatgaagga
    16201 ccccagaagc cggtgccatt ctatgaacgt gaggaggaga atgagaccgg ggaagattcc
    16261 ggagaagtag ccgcggttga tcctgtgacc ggggccggct ccgcagccgt aactgattct
    16321 gtgaccgaag ctggattcaa aaccataacc cgcgcaggac cagcgcctga atccaggtct
    16381 aaataaaaca ggcccgggcc cggggtttaa taaaatgaga tttagcctgc gaatcaagga
    16441 aattaattgt taattttaac gccacttaac ccaacctaaa ggcccggtaa tgtatgtcag
    16501 aactggaact ccggcatttg atgagc
//
LOCUS       DAOPBK010000039        16467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3581_length_16467_cov_6.530283, whole genome shotgun sequence.
ACCESSION   DAOPBK010000039 DAOPBK010000000
VERSION     DAOPBK010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16467
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3581_length_16467_cov_6.530283"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1628
                     /locus_tag="PLA17_03760"
     CDS             291..1628
                     /locus_tag="PLA17_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HPK84560.1"
                     /translation="MRRTLLTILLSLVIASLGAHPWKPRHYVIIDSDGGIDDLKAICM
                     LMASPDVRILAITASDGFHKAPVAYGKLRGLADGLHHEGLPVAGPENAVTLIEKMLAS
                     ETTPVKFLSLGSLKTAAEAMEQAPSFAGKVKQIYWSNSGLPGKEGSNYKSDPASAEKV
                     LAGKIPVTVTGGGGDGFYDVPLLDAISHIHSPYAEKARELINSMPSHSFVYTAFDEMV
                     PLLLHFPELFSPEGSDESISYRTPSDIDGLRTAALKMLRGETVERMQTIKRMPSDTSF
                     YMPDIQPFVTEIRNRYGEDEWTSGVIANELHRHLGVFAIIGVKMGIRAREYFCTGVDE
                     MEVTTLAGSTPPLSCMNDGIQVSTGATPGHGLLTVSRQQPCLPAADFTHKEQTIRITL
                     KKELADRVSAELKEINFIYGLDSDIYWELVRNNSIKYWLQFDRHEIFDIEVIK"
     gene            1637..3970
                     /locus_tag="PLA17_03765"
     CDS             1637..3970
                     /locus_tag="PLA17_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HPK84561.1"
                     /translation="MKPPILICILAVFMQMVACSNEQATAPAVIPQPVEMVLSHGWFR
                     LAPDTRISWSGSQEASAVAAQLASVLRPATGFELIVSEGIDGDISLLIDASEEWKPEE
                     YRLTVEKNKVIITAGSGAGLFRGIQTLRQLLPPQAFSHNRAEGVKWAIPCVRVTDYPR
                     FGWRGMHLDVSRHFFDKEFVKKYIDILAMHKLNVFHWHLVDDQGWRIEIKKYPELTEV
                     GAWRVDREDKPWDSRQPQQPGEKATYGGFYTQDDIREIVAYAADRFITVVPEIEMPAH
                     VGSAMAACPEYSCTGGPFTVPPGGVWPITDIYCAGKEETFAFLEDVLTEVIDLFPSEY
                     IHIGGDEADKTEWKRCRDCQARMRKEGLKDEHELQSYFIKRIELFLNSKGRRLIGWDE
                     ILQGGLAPEATVMSWQGFEGGIAAARSGHKAVMVPTSHCYLSVYQGDPVTEPESFRGL
                     LTLKKVYSFEPVPPELTAEEAELIIGAQGCLWTEYITDGTRAEYMILPRLSALSEVIW
                     SPSDRRNWEEFNIRLPKMLERFDAAGINYSRGSYRVDMYASYDEASGDIILEMASEQP
                     SPEIRYTTDGSDPLPGSEEYSGPLKMTGKTTVKAAIFENGKMIGGISSKAININKATG
                     MKVQYNIPFSDKYRGHADRTLVNGINGSGDFNDGHWQGFEGTDMDVVIDLGDETTISS
                     ISSGYMAAVGSWIFLPRSVEYSCSNDGNDYTPLGTVITEVDPADQGNMIEVYSSSFPA
                     VNTRYVRIVARGLITCPPWHAGAGNKAWLFCDEISVE"
     gene            complement(4123..5052)
                     /locus_tag="PLA17_03770"
     CDS             complement(4123..5052)
                     /locus_tag="PLA17_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HPK84562.1"
                     /translation="MNLTYIDFLVSAVLAAIMFGVGLSINFSDLKEIRRSPKAFILGL
                     VSQMILLPLIVFLIIGFTDLPPTLKVGFIILAACPGGTTSGFVTVLFRGNVALSVSLL
                     AINSLLTLLTIPLLVNLALTVFIGRHSPFELPFVESFLQIFLISILPAMAGIVLKYFR
                     EEIANRIQKPVRNVMIILFAGVYAIIAFAKESSGGSGITTEDFWKIFPYAVLINFAGF
                     AAGMAMGLFGKTGLRTSWTIGVEVALQNTTLALLVAGTLIQSNEMVKPALVYALFSFW
                     TALLYGVSVKWYDGSRLFGEFREIREKKINGNR"
     gene            complement(5187..6443)
                     /locus_tag="PLA17_03775"
     CDS             complement(5187..6443)
                     /locus_tag="PLA17_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPK84563.1"
                     /translation="MKNKFLSLAIVVVAGTMALMASCEDQTAKILPTDPVEIELTLKQ
                     KEVVALTNRFAFDLFKPVVAGEGSGTNIMISPFSITSALSMTLNGAAGETFNAVRSAL
                     RYDGQSLEEINDTWRKLVTEMVPVDERVVMEIANSVWAENNFPVKKDFIDALKEWYLA
                     EARNFDITDPGSVDIINGWIEEKTHDRIQNMLSSLDRDLVMLLINAVYFNGKWKHQFD
                     VKNTTERPFFVTPGNPVNVPVMYQKQKFSMSRPEKVTLVELPYGQGNYSMVVALPDEG
                     IALSEIVTGLDAGKWEEWMESLSYGPTEVELYMPKFRYEYKRKLNDDLISLGMGRAFD
                     PGTADFSRISDEEIFISFVLHQTFIENKEEGTEAAAATVAGFTRTSLPPEPEVIDINR
                     PFLYFIRETTTGTIVFMGLVADPTGV"
     assembly_gap    6648..6747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7046..7837
                     /locus_tag="PLA17_03780"
     CDS             7046..7837
                     /locus_tag="PLA17_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HPK84564.1"
                     /translation="MSTLTNEELIRYQRHLTIAEIGGKGQELLKKASVLVIGAGGLGS
                     PVLMYLAAAGVGRIGIVDSDEVTTGNLQRQILYETGDVTRPKAETAAKKLRRINPHTE
                     VLAYPVRFTEENAGRLMAGYDIAVDCCDNYSTRYVLCDATLKAGIPMVYGAVREFMGQ
                     ASVLNYRGGPTYRDLYPEDMLPEREERSVAPGVGSSVPPGVESSAATGVESSVAPGVT
                     GPLPGIIGAVQACEVIKIITGAGEILSGRLFQADMLNNRIDIITL"
     gene            complement(7827..8834)
                     /locus_tag="PLA17_03785"
     CDS             complement(7827..8834)
                     /locus_tag="PLA17_03785"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK84565.1"
                     /translation="MNCFLTGSNGFIGRRLVEELSAGGNKVKCLVRSPDKFRALSSLP
                     GVTPVVGDLDAVDALDEALPGCDTVFHLAAYAKPWSKDRSLPYRINVAGTENVLKAAL
                     KAGVRRFVFTSSAAVIGPSPGTDPIDESFPRTVPWFNEYEETKAVAGELVRSYNRDGM
                     ECVIVNPTRVYGPGPINESNSVTKMISLYARGRWRIIPGDGKSVGNYVMVDDVVRGHI
                     LASQKGRPGEQYILGGENLTFDRFFETLATLTGRRLWLVRVPVSLMSAVAGVMEWQAS
                     FTGIPPLITPPWIKKYLCHWSLSSGKAEAELGYHPVSFADGARTTLEWLGESITSCFK
                     G"
     gene            complement(8922..10862)
                     /locus_tag="PLA17_03790"
     CDS             complement(8922..10862)
                     /locus_tag="PLA17_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84566.1"
                     /translation="MKHRLALAAALLAIVIAASGQTGKVEHSVYVPIDPQVVQDQDEM
                     TWDDYRPIPGKNWADPALKPERGFRMALVAVDFSDQPFVMTLPKGSDLFGNPQIDPVD
                     RSEIPQFYADFWLRPSAVNYGQTINGYWMEQSRGKFGITQLEAFGPYRMPKPLWAYGL
                     SEWGQNSATPDGSKVNYRMERDIDSLWRADKGDIKGNYDAILRVYAGYDETGVWQEFG
                     EMKFNSRDEIPAEWGNPNPDMPRWAPTRYVEWTSWKAGQMQWGLSSIRQGENSGTITH
                     ELGHFAFRTGDNNNNPYIKPYRRAGSGPWDMMDRGSFNGPGGPHMRWVVPPIAGAAMP
                     AGLMLRNRLVNGFITEHDIITVSREGLASLAPVIARITARAVQPLPGEYAGMMVRLDG
                     AEPHDRTPVTDPATDPLSPGKPRFNYYSLEVVQRIGYDSFCPDNGVLMAMNTDGEGSN
                     GGPNGFNCFNWVIDAHPEDINMVDYVKPDGEKVMRTVADYRQLNDALFHAGLNSGSEF
                     EYVDTHNRLHFYIIDIHRNEQEILSYTVGVRSLDSDVVREPVTVSAGKAAQKIAGEGI
                     VEFAVSGDDLCRLYAVVTGDGWEVKLFNEIVAPGDGKTVKVPVYVRAKQGCSKKAEIT
                     FSAVSESDSKYRSAASIKVKLQ"
     gene            complement(10892..12394)
                     /locus_tag="PLA17_03795"
     CDS             complement(10892..12394)
                     /locus_tag="PLA17_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPK84567.1"
                     /translation="MQAQQNKVVDRIIELGQTDNRTMHHLDVISNRFGGRLVGSDAYE
                     NAAEWCASQFRSWGMEVIMDEAGTVPVGFNRGPWFGRMLSENGMILHFATPSYTAGTK
                     GVQRGHVLLEPKSRDEFERMKGALKGAWVLIGGKNEGWPVDCSPSGDSLRAAAMVYND
                     SVALLNREIMRENMMNRDAPPKELHKLREVPALFYREMCEAGILGVIQSSEVPIRALY
                     DRKNIDRISFDNLPTVCDIKLDEHQYDIIAQMARERRYFLLEFDIRNHFKPGPVKYYS
                     VIGVIKGKKYPNEYVMMGGHLDAYDVATGGVDDGSGVSPVMEAARLIMAAGGKPDRTI
                     LACLWAAEEFGLVGSKHWVETNMEKLPKISNYFNRDGGPMPNSGITVPPAMYDDFEKI
                     CKPLNSINPDFPFTLRKRTDPPRPRPASAGGSDHAYFAINGVPTLNFDGPDVKGYNFN
                     YGEIWHTERDTYDKSISEYQEHTSVVTAVVVYGLANLDHLLSREGLYREE"
     gene            complement(12567..13259)
                     /locus_tag="PLA17_03800"
     CDS             complement(12567..13259)
                     /locus_tag="PLA17_03800"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPK84568.1"
                     /translation="MKKLVILTGAGMSAESGIKTFRDSGGLWEEHKVEDVATIEGWYR
                     NPDLVQKFYNDRRRQLEKAVPNPGHTGLKALEKLFDVHIITQNVDNLHERAGSKNIIH
                     LHGLLTQARSSKNPDMVIDIGYRDISPGEQAADGSLLRPNIVWFGEAVPAMEEAADIA
                     SQADLFAVIGTSLNVYPAAGLIHYVPARTPVFLIDPKPVSVYTNHKVDYISEGAGKGV
                     AILAEKLLDFTT"
     gene            13628..14320
                     /locus_tag="PLA17_03805"
     CDS             13628..14320
                     /locus_tag="PLA17_03805"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPK84569.1"
                     /translation="MKNLLIILALVAFLPSDQEEVRYTQARSRMVKSQIAARGISDPA
                     TLRAMERVPRHLFVPAALAGSAYSDQPLPIGYNQTISQPFIVAYMTELSKPAKGKKAL
                     EIGTGSGYQAAVLAEIVDTVYTIEIVPELAREAASRLKVLGYDNIVVRYGDGYKGWPE
                     HSPFDIILVTAAADHIPEPLKEQLAEGGRLVMPVGNPATFQELIVLTRRKGRITEQRL
                     APVRFVPLKRLK"
     gene            14385..14948
                     /locus_tag="PLA17_03810"
     CDS             14385..14948
                     /locus_tag="PLA17_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPK84570.1"
                     /translation="MIYVIIAAAVILLLSALLIFNSLIRKKNDVENAFASIDVMLRKR
                     YDLIPQIVETVKAYMNYERELLTKITELRIRAMSQTLTAEERVQVENRLGTSLTDLLI
                     AVENYPDLKASTSFLQLQATLNEVEEQLAASRRAFNAAVTVYNNSIEVFPSNIIASMM
                     RYKRRSLFEIPAAKREEVSSIPPVNMV"
     gene            14963..15925
                     /locus_tag="PLA17_03815"
     CDS             14963..15925
                     /locus_tag="PLA17_03815"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HPK84571.1"
                     /translation="MSRYGTFREFYEKELLPDLESIDMRRRATNKKVLTVLFVTAAAV
                     LAELLLIPDNTDFPKSAPMIITAFAGLVAAGIVSKNYRKEYKEKIIARIAGYAEEGLT
                     YKPDASIARYDFVKSRIFTLSCDRYSGEDYFRGRIGKTETEFSEVHAMHRNTSGTGSQ
                     QRDNYTTIFKGIFIIADFNKNFRTHTVVLPDTAEKLFGKFGQSLQSVSFGRGKLIRLE
                     DPRFEKEFCVYGDDQIESRYILTPSLMERILAFREKWKTKVYLSFLDSKVYIAISMNR
                     DLFELRPFKPAADFDFLEETLRFLTLLTGVVDDLNLNTRIWTKE"
     gene            16039..>16467
                     /locus_tag="PLA17_03820"
     CDS             16039..>16467
                     /locus_tag="PLA17_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84572.1"
                     /translation="MEKLNLFFDRIKDLSFWQRLFQWRRVRNLSFDAYEEFRALARET
                     EAVRETAGRLQNRITELSAHNENLSRRVHEMEVQSARKDANIEELNGRLREDSASIQE
                     LTKKVAGFEATREDNVKNYNENIARLNQTKESLEAERRRLN"
BASE COUNT         4244 a         4290 c         4023 g         3810 t
ORIGIN      
        1 agtgaacacc ctgcttccag gaggtggttg cctgcgggtg aacatggcac acagcgcaga
       61 aatcattggt tgaggtcacg tctacagcct tgccgaatat ggcaaggata accattccgg
      121 tcacaaggcc gataaaaaac agtagccagg ttttcctctt cacataatgg aaattaacgt
      181 tgtcaatata gtaaaaaaga gtttttcttc gacagccggc gaaccacact ccgggagatt
      241 ccgtcagaat tttcaatgct aatttgctat ttttggcaaa aagcaaagaa atgagacgca
      301 cattactcac catacttctt tcacttgtta ttgcttctct cggggcacac ccctggaaac
      361 caagacatta cgttattatc gacagtgacg gcgggattga tgacctgaag gcaatatgca
      421 tgctgatggc atcaccggat gtaaggattc tggcaataac ggcatcggac ggatttcaca
      481 aggctcctgt ggcttacggg aagctaaggg ggctggccga tggacttcat catgaaggcc
      541 ttccggtggc cggaccggaa aatgcggtca cgctcattga gaagatgctg gcttcggaga
      601 caacaccagt caaattcctg tcgctgggca gccttaaaac cgcagcagag gcaatggagc
      661 aggcaccctc attcgccggc aaggtgaagc agatttactg gagcaacagc gggctaccgg
      721 gaaaagaggg aagcaattac aaatctgatc cggcgtcagc ggaaaaggtt ctggcgggga
      781 agattccggt aactgtgaca ggaggcggag gtgatggttt ctatgacgtt ccactcctgg
      841 atgccatcag ccacatccat tctccatatg cagaaaaggc aagagagctg ataaactcaa
      901 tgccatcgca cagctttgtc tataccgctt tcgacgagat ggtacccctg ctcctgcact
      961 tcccggagct tttcagcccg gagggcagtg atgaaagtat atcataccgc accccgtcag
     1021 acatagacgg gctccggaca gcagcgctta aaatgcttcg cggcgagacc gtggaaagaa
     1081 tgcagaccat aaaaaggatg ccatctgaca catcgtttta catgcctgat atccagcctt
     1141 tcgttactga aataagaaac aggtatggtg aggatgagtg gacctcggga gtgatagcca
     1201 acgaactgca tcgccacctt ggagtatttg caattatcgg agtaaagatg ggcatcaggg
     1261 cacgcgaata tttctgcacg ggagttgatg agatggaggt gaccaccctt gccggatcaa
     1321 ctcccccgct aagctgtatg aatgacggca tccaggtcag caccggagca acaccggggc
     1381 acggactgct gacggtcagc cggcagcagc cctgcttacc ggctgccgac ttcacccaca
     1441 aggagcagac cattcgtatt acactgaaga aggagcttgc cgaccgggtc tccgcggaac
     1501 tgaaagagat taattttatc tacggactcg acagtgatat ctactgggaa ctggtcagaa
     1561 acaactccat caagtactgg ctgcagtttg acagacatga gatatttgac atcgaagtaa
     1621 tcaaatagat gatattatga agccgccgat tctgatctgc atcctggcag tattcatgca
     1681 gatggttgca tgcagcaatg agcaggcgac tgctccggct gttataccac aacctgttga
     1741 aatggtcttg tcgcacggct ggttcaggct tgcacccgat acccgcattt catggtcagg
     1801 cagtcaggaa gcgtcagccg tggccgcaca gcttgcctcg gtgttgcgcc ctgcaacagg
     1861 ttttgaactg attgtgtcgg aaggaattga cggtgatatc tcgttactga ttgatgcctc
     1921 tgaagagtgg aaacccgaag agtaccggct gacagtagaa aaaaataaag tgataatcac
     1981 tgccggatca ggagcgggac tgttcagggg aatccagaca ctgagacagt tgctgccgcc
     2041 acaggcattc tcgcacaacc gggcagaggg ggtgaaatgg gcaattccct gtgtcagagt
     2101 gacagactat ccgcgctttg ggtggagagg aatgcatctc gacgtcagca ggcatttctt
     2161 tgacaaggag ttcgtgaaaa agtacatcga tatcctcgcc atgcataagc tgaatgtttt
     2221 ccactggcac ctggtagatg accagggatg gcggatagag ataaaaaagt atcctgagct
     2281 cactgaggtc ggagcctggc gcgtcgaccg cgaggacaaa ccctgggact ccaggcagcc
     2341 tcagcaaccc ggcgaaaagg caacatacgg cggcttctac acacaggatg atatcaggga
     2401 gatagtggca tatgcagctg atagatttat cacagttgta ccggagatag agatgccggc
     2461 gcatgtcggg tcggccatgg ccgcctgccc ggaatactca tgcacgggag gccccttcac
     2521 cgttcctccc ggcggggttt ggccaataac ggacatatac tgcgccggca aggaggagac
     2581 attcgctttc cttgaggatg tgctgaccga ggtgattgat ctcttcccgt cagaatatat
     2641 ccatattggt ggcgatgagg ctgataaaac ggagtggaaa cgttgtcgcg actgccaggc
     2701 acgtatgcgg aaagaaggat tgaaggacga acacgaactg cagagctatt tcataaaacg
     2761 aatcgagctg tttttaaact ccaaaggccg caggcttatc ggttgggatg agatactcca
     2821 gggcggactg gctcccgaag ccactgttat gtcatggcag ggattcgagg gaggcatcgc
     2881 agccgcaaga tcaggccaca aggcggtaat ggtccccacg tcacactgct atttgagcgt
     2941 ttaccaggga gaccccgtaa ccgaaccgga atcgttccgc ggattgctga cactcaaaaa
     3001 ggtctactcg tttgagcctg tacccccgga acttaccgct gaagaagcag agctgatcat
     3061 cggggctcag ggatgcctct ggactgaata cattactgac gggacaagag ctgaatacat
     3121 gatactgcca aggctgagcg cactttcaga ggtaatctgg tccccgtcag acagacgaaa
     3181 ctgggaagaa ttcaacattc gcctcccgaa gatgttggaa aggtttgacg cggcaggcat
     3241 caactactcg cggggttcat accgtgtcga catgtatgct tcatatgacg aggcatcagg
     3301 agacatcata ctggaaatgg catcggagca gccctcccct gaaatcaggt acacaaccga
     3361 cggatccgat cccttgcctg gttccgaaga atattccggg cccctcaaga tgacgggaaa
     3421 gaccactgtg aaggcagcaa tttttgaaaa cgggaaaatg attggcggaa taagctcgaa
     3481 agcaatcaac atcaacaagg ccaccggtat gaaggtgcag tacaacattc ccttctctga
     3541 caagtacagg ggtcacgctg accgcacact ggtcaatggc ataaacggca gcggcgactt
     3601 caacgacgga cactggcagg gatttgaagg aaccgacatg gatgtggtca tagatctggg
     3661 tgacgaaaca acaatatcat caatatcatc gggttacatg gccgccgtcg gaagctggat
     3721 cttcctgcca cgcagcgttg aatactcctg ctcgaatgac ggcaacgatt atactcccct
     3781 gggcacggtg atcacagagg ttgaccctgc cgatcagggc aacatgattg aggtatattc
     3841 ttcatccttt ccggccgtaa acacaagata tgtccgtata gtggcgcgtg gtctcataac
     3901 ctgcccgcca tggcatgccg gcgccggaaa caaagcatgg ctcttctgtg atgagatcag
     3961 tgtggaatga tgcacgggtt actattcccg gtgcttatcc acaggcgggt catacagcat
     4021 tgtatatggc cgggcgaaac cattgatgac gtaaggaata atgagaacgt ctgacgtgtt
     4081 gaggtgaagc tgttcaatgg atgttaagtg ctgaaacggt aatcaacggt tgccgttaat
     4141 ctttttttcg cgtatctcgc gaaactcgcc gaacagtctg gagccgtcgt accacttgac
     4201 agacacaccg taaagcaacg ctgtccaaaa agagaagagt gcatatacaa gtgccggctt
     4261 gaccatttca ttgctctgta tcagtgtgcc ggccaccagc aacgccaggg tggtattctg
     4321 cagggccacc tcaacaccta ttgtccatga tgtcctcagc cctgtttttc cgaacagtcc
     4381 cattgccatc ccggcagcaa atccggcaaa attaatcaga acagcgtatg gaaatatctt
     4441 ccagaagtcc tctgttgtaa tccctgaacc accgcttgat tcctttgcaa aagctataat
     4501 ggcatagact cctgcaaaca ggataatcat cacattccga accggcttct gaatcctgtt
     4561 ggctatctcc tccctgaagt acttcagcac tatcccggcc attgccggca gtatggatat
     4621 aaggaaaatc tgcaggaatg attctacaaa cgggagttca aaaggagagt gcctgccaat
     4681 gaataccgtc agcgcgagat tgaccagcag cgggatggtt aacagtgtca ggaggctgtt
     4741 tatggcaagg agtgataccg agagagccac gttacccctg aacaatacag tgacaaaacc
     4801 tgaagtcgtg ccgccggggc atgctgcaag aatgataaaa ccaaccttca gggttggagg
     4861 gagatcagta aaacctatta taaggaatac tatcagcggc agtagtatca tctgtgagac
     4921 aagcccaagg ataaatgcct tcggcgaccg gcgaatctcc ttcagatcgg agaaattgat
     4981 tgacagcccc acaccgaaca tgatggcagc cagcacggca ctgacaagaa aatcgatata
     5041 ggtcagattc atcactgtaa agaaaatgga ttttcaccac atgacaataa acaatcaggt
     5101 cctgaattgc tgaaccggaa agccacttcc agcccggaaa tgagtcagca taattcagtc
     5161 attgactatt cgcaatacgc aaatcctcac acccccgtgg gatcagcaac caggcccatg
     5221 aatacgattg tgccggtggt tgtctccctg ataaaataga ggaacggcct gttgatatct
     5281 atcacctcag gctcaggagg aagtgatgtg cgggtaaacc ctgctacagt agctgcagca
     5341 gcttcagtac cctcttcctt gttttcgata aatgtctggt gaagaacaaa tgagatgaat
     5401 atctcctcgt cacttatccg gctgaagtca gcagtgccgg gatcaaatgc ccggcccatc
     5461 cccaggctga tcagatcatc gttcagctta cgcttgtatt cgtacctgaa tttcggcatg
     5521 tacaactcaa cctcagtcgg cccgtatgac aggctttcca tccactcctc ccacttgccg
     5581 gcatcaagtc cggtaacaat ctccgaaagg gcgattcctt catcaggaag agccaccacc
     5641 atcgagtagt taccctgccc gtacggcaac tcaaccagcg tcaccttctc cggacgcgac
     5701 atggagaatt tctgcttctg gtacatcacc ggcacattta caggattacc gggagtgaca
     5761 aaaaacggac gctcagttgt attcttaacg tcaaactgat gtttccactt gccattgaag
     5821 taaaccgcat tgataagcag catcaccaga tcacggtcaa gtgatgaaag catattctga
     5881 attctgtcgt gggtcttctc ttctatccag ccattgatga tatccactga gcccggatcg
     5941 gttatgtcaa aattccgggc ttcagccagg taccactcct tcagtgcatc aatgaaatcc
     6001 ttctttaccg gaaagttgtt ttcggcccat accgagttgg caatttccat tacaacacgc
     6061 tcgtccaccg gaaccatctc agtcacaagc ttgcgccagg tgtcgttgat ctcctcaaga
     6121 ctctggccgt catagcggag agccgatctg accgcgttga atgtctcgcc ggcagcgccg
     6181 ttcagagtca ttgaaagtgc actggtgatg ctgaaaggag agatcatgat gttcgtacct
     6241 gatccctctc ctgccaccac aggtttgaaa aggtcgaagg caaacctgtt tgtcaatgcc
     6301 acaacctctt tctgtttcag ggtaagctct atctccacgg gatcggtcgg gagtatcttt
     6361 gccgtttgat cttcacatga tgccatcagt gccatcgtgc ctgcaaccac aacaattgcc
     6421 agacttaaga atttattttt cattatttcc tctgttaagg tacaacattt atgtacaaat
     6481 atgataccaa cagcaagaga gtcccgctcc cggccggagt acttctccac ctgaatcctc
     6541 tcccaaaaag aatcccgctc ccggccggag cgctctttca cccggttcct ctcccgaaga
     6601 gagtcacgct cccggccgga gtacttctcc aaccgggtcc ctgccagnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnccc gaagagagtc acgctcccgg ccggagtact
     6781 tctccaaccg ggtccctgcc agcaaataaa tcttttgtta tacagtctgc ctgattgtgg
     6841 aaagagcagc caggcttaat gcaaaccatc ctggccaccc ggcagataat gagaggtgat
     6901 atcatgattt tttcataagt tagcgcatag attcgatatt tctgaaattg gaaaacgcaa
     6961 cactccggca tttatggaaa tgatccgcaa acagctggaa acgaccgaaa cctgtgcaga
     7021 ctgagaataa caaacccggg cagaaatgag tacacttacc aatgaagaac ttatccgcta
     7081 ccagaggcat ctgacaatag ccgaaatcgg cgggaagggg caggagttgc tcaaaaaggc
     7141 atctgtcctg gtgattggtg ccggcggcct gggatcaccg gtgctgatgt atctcgcagc
     7201 tgccggggta ggcaggatag gcattgtaga ttctgatgag gtcactaccg gcaacctcca
     7261 gcggcagatt ctctatgaga caggcgacgt gaccagaccc aaggccgaaa ccgccgcgaa
     7321 aaagctcaga agaatcaacc ctcatactga ggtgctggca tacccggtgc ggttcactga
     7381 agaaaatgcc ggcaggttaa tggccggata tgatattgcc gtcgattgct gcgataacta
     7441 ttcaacccgg tatgtcctgt gtgacgcaac cctgaaggct ggcataccga tggtttatgg
     7501 cgccgtaagg gagttcatgg gacaggcttc ggtcttgaac tacaggggtg gccccacata
     7561 cagggatctc taccccgaag acatgctccc ggagagggaa gagagatcag tcgctcccgg
     7621 tgtagggagt tctgttcctc caggtgtaga gagttcagcc gccaccggtg tggagagttc
     7681 agtcgctccc ggtgtgacag gcccccttcc cggcataata ggtgcagtac aggcatgcga
     7741 ggtgatcaag ataataaccg gtgccggtga aatactctcc ggccgcctgt ttcaggcaga
     7801 catgctcaac aatagaatcg atattattac cctttgaaac agctggtaat gctttccccc
     7861 agccactcaa gagtcgtcct ggctccgtca gcaaatgaaa ccggatgata tccaagttca
     7921 gcttcggcct tgccgctgct caggctccag tgacacaggt atttcttgat ccatggaggg
     7981 gtgatcagcg gcggtatgcc cgtaaacgac gcctgccatt ccatcacccc ggctactgcc
     8041 gacatcaggc tgaccggaac ccttaccagc cacaggcgcc tgccggtaag agtggcaagg
     8101 gtctcaaaga agcggtcaaa ggtcaggttc tctccgccca gtatatattg ttcacccggc
     8161 ctgcccttct gagaagccag aatatgccct ctgacaacat catcaaccat gacgtaatta
     8221 cccacgcttt tgccgtcacc cggaatgatg cgccatctac cccgggcata aagggaaatc
     8281 atcttcgtga ccgagttgct ctcattgatc ggtcccggac cgtaaactct tgtcggatta
     8341 acaatcacac attccatccc gtcgcggttg taagacctta ccagctcccc ggccactgcc
     8401 ttggtctcct catactcatt gaaccatggc actgtacgtg ggaatgattc atctatggga
     8461 tcggttcccg gcgaaggccc tatcactgca gccgaagagg tgaagacaaa acgcctgacc
     8521 cctgctttca gggcagcctt cagcacgttc tccgttccgg cgacattgat cctgtatgga
     8581 aggctcctgt ccttagacca gggtttggca tacgcagcaa ggtgaaaaac cgtgtcacaa
     8641 cctggcaaag cttcatcaag tgcatccacg gcatcaaggt cacccacaac aggtgttact
     8701 cccggaaggg aggagagagc cctgaactta tcaggcgacc gtacaaggca tttcacctta
     8761 ttcccgccgg cggagagctc ctcaaccagc ctcctgccaa taaaaccgtt cgatccggtc
     8821 agaaaacagt tcataccagg ctcctttcag atatcaaata tacctgaacg aagccacaaa
     8881 tcaggctgtt caaacatcaa ttccgtgaaa aaccggataa actactgcaa tttcaccttt
     8941 atcgaggctg ccgacctgta tttactgtca ctttccgaca cagcggaaaa ggttatttca
     9001 gcctttttgc tgcagccctg ctttgccctg acataaacgg ggaccttcac tgttttgccg
     9061 tcaccggggg caactatctc attaaacagt ttaacctccc atccgtcacc cgtgacaacg
     9121 gcgtacaacc tgcacagatc atctcctgaa acagcgaact caacaattcc ttcaccggct
     9181 atcttctgag cagcctttcc ggcagaaacg gttaccggtt ccctgacaac atccgaatca
     9241 agtgacctca ctccgactgt gtatgagaga atttcctgct catttctatg aatgtcaatg
     9301 atatagaaat ggagcctgtt gtgggtgtca acatattcaa actcgctgcc tgaattcagt
     9361 cccgcgtgga aaagcgcatc attaagctgc cggtagtctg ccactgtacg catcaccttt
     9421 tcaccatcgg gcttaacata atccaccatg ttgatatcct cagggtgagc atcaattacc
     9481 cagttaaagc aattgaatcc gttggggccg ccgttgcttc cctccccgtc ggtgttcatt
     9541 gccatcagca ccccgttgtc agggcagaag gagtcatacc cgatacgctg caccacttcg
     9601 agggagtagt aattaaacct tggcttgccc ggagagagcg ggtctgtagc cggatcggtc
     9661 acaggtgtac ggtcatgagg ctcagcgccg tctagccgta ccatcatacc cgcatattca
     9721 ccaggcaatg gctgaacggc gcgtgccgtg atccgtgcaa tcacaggtgc caaagaagcc
     9781 agcccttcac ggctcacggt aataatatca tgttcggtaa taaacccgtt cacaagcctg
     9841 ttgcgcagca tcagccctgc cggcattgct gcccccgcta tgggtggcac cacccacctc
     9901 atatgagggc cgccggggcc attgaatgaa ccgcggtcca tcatgtccca gggccctgat
     9961 ccggctcgtc tgtaaggttt gatataggga ttattgttgt tgtcacctgt tctgaaggca
    10021 aagtgcccca gctcatgggt gatggtgcct gaattctcac cctgccgtat ggatgaaaga
    10081 ccccattgca tctggcctgc cttccaggaa gtccattcaa cataccgtgt gggagcccag
    10141 cgcggcatgt cagggttcgg attgccccac tcagccggaa tctcatccct gctgttgaac
    10201 ttcatctcac caaactcctg ccatacacct gtctcatcat accctgcata tactctcagg
    10261 atggcatcat aattgccttt tatgtcacct ttgtcggcac gccagagaga gtcaatgtcg
    10321 cgctccatcc ggtaattcac ctttgatccg tcaggtgtgg cactgttctg cccccactcg
    10381 ctcagcccgt atgcccacag tggtttcggc atacggtatg gaccgaaggc ttcaagctgt
    10441 gttattccga actttcccct cgactgttcc atccagtatc cgtttatggt ctgaccataa
    10501 ttgacagccg aaggcctgag ccagaaatca gcataaaact gcggtatctc acttcggtct
    10561 accggatcaa tctgaggatt tccaaaaagg tctgatcctt tgggcagggt catcacaaaa
    10621 ggctggtcag aaaagtcaac ggctaccagt gccatccgga acccacgctc aggcttcagg
    10681 gccggatcag cccagttctt gccggggatc ggcctgtagt catcccaggt catctcatcc
    10741 tgatcctgta ccacctgcgg atctatcgga acataaacag agtgctcaac ttttcctgtc
    10801 tggccggaag ctgcaatcac tatagccagc aatgcagcag caagagctaa tctgtgcttc
    10861 atcacctctc ccttgtttta attattgact attactcttc cctgtaaagc ccttcccttg
    10921 acagcaggtg atcaagattg gcaagaccgt aaactactac agcagttacc actgaagtat
    10981 gttcctggta ctccgagatg ctcttgtcat atgtgtcgcg ttcagtatgc catatctccc
    11041 cgtaattgaa attgtaaccc ttgacatccg ggccatcgaa attaagcgtg ggaacaccgt
    11101 ttattgcaaa atatgcatga tcagaaccgc ctgccgaagc cggacggggc cggggcggat
    11161 cagtacgttt cctgagtgtg aaggggaagt caggattgat gctgttgagc ggtttgcata
    11221 ttttttcaaa atcatcgtac atggctggcg gaacagtaat cccggagttt ggcatcggtc
    11281 ccccgtcacg gttgaagtag ttggagatct tcggcagctt ctccatattg gtttcaaccc
    11341 agtgttttga accaaccagt ccgaactcct cggcagccca gaggcatgcc aggatggtgc
    11401 ggtcaggctt gccgccggca gccattatca ggcgtgccgc ttccatgacc gggctgacgc
    11461 ctgatccgtc gtccacacca ccggttgcaa catcatatgc atcaagatga ccgcccatca
    11521 tcacatactc attcgggtat ttcttgccct tgattacacc tatcacactg taatacttca
    11581 ccggcccggg tttgaagtga ttccttatat cgaactcgag gagaaaatat cttctctcac
    11641 gggccatttg tgcaattata tcatactggt gttcatcaag cttgatatcg caaactgtag
    11701 gaaggttgtc aaatgagatc ctgtcgatgt tcttcctgtc atacagcgcc ctgatgggta
    11761 cttcagacga ctgaatcacg ccaaggattc ctgcctcgca catctcacga taaaatagag
    11821 caggcacctc cctgagctta tggagttcct tcgggggtgc atccctgttc atcatgttct
    11881 cacgcatgat ctccctgtta agcaacgcga cggagtcatt gtaaaccatg gcggcagccc
    11941 ttaggctgtc gccactgggc gagcagtcaa caggccagcc ctcattcttc cctccgataa
    12001 gcacccaggc tcccttcagt gcgcccttca tcctttcaaa ctcatccctg cttttgggct
    12061 cgaggagtac atgtccgcgc tgaactccct ttgtgccggc agtatatgaa ggagtggcaa
    12121 aatgcaatat cattccgttt tcagagagca tcctgccgaa ccatgggccg cggttgaaac
    12181 caaccggaac tgttccggct tcgtccatga tcacctccat gccccagctt ctgaattgtg
    12241 acgcacacca ttcagctgca ttctcatatg catcggaacc aacaagcctt ccaccgaaac
    12301 ggttggaaat gacatcaagg tgatgcatcg tacggttgtc tgtctggccc agttctatga
    12361 tccggtcaac aaccttgttc tgctgagcct gcatccctgt agcaagcaaa atcagcaata
    12421 agggaagaaa taattttttc atgtcactcc ttttggattt ttagcaccgc caaactataa
    12481 attaaggtta agaaatagaa ggacaacagc accttattat gatgccggat agttaaaaaa
    12541 agttatattc tgaccggtgt gctgccttaa gtagtgaaat ccaaaagttt ttcggccaga
    12601 atggcaacac ccttgcccgc accctcggaa atataatcga ccttgtggtt tgtgtaaact
    12661 gaaaccggtt tcggatcaat cagaaaaacc ggtgttcttg ccggaacata gtgtatcagc
    12721 ccggcagccg gataaacatt caacgatgtg ccaatcacgg cgaaaagatc agcctgtgag
    12781 gctatgtctg ccgcttcctc catggcaggt acagcctcac caaaccagac tatgttcgga
    12841 cgcagtaatg acccgtcagc agcctgttct cccgggctga tatcacgata gccaatatca
    12901 atgaccatgt cagggttctt gctgctgcgc gcctgtgtca gcaacccgtg aagatggatg
    12961 atgtttttac tgcctgctct ctcatgaagg ttatcaacgt tctgtgtgat aatatgcaca
    13021 tcaaacagct tctcaagcgc tttcagaccc gtatggcccg ggttaggcac agccttctcc
    13081 aactgacgcc ggcgatcatt gtaaaacttc tgcaccaggt caggattacg gtaccatcct
    13141 tcaatggttg ccacatcctc aaccttgtgc tcctcccaaa gacctccgct gtcacggaat
    13201 gttttaatgc cgctctctgc gctcattccg gctcccgtta agatgaccag ttttttcatc
    13261 ataatataat tttgatttta acgacatgat ggaatctgca tgaccgaaaa tcaaataaaa
    13321 aggtgtttgt gtatttcaac agtccggtat cattttcaaa caactgttca taactgtctg
    13381 attatcagga gtggtcattt cttttccaat tgatcactcc aattaattgg taatgtgcct
    13441 gttggcaaac acttgcaggt ctgttatctg tttgacacat gcaggtttca tgtcatgaat
    13501 ttaagaaaaa acggggaaat aaaaaatgcc ggagagcagg gtatttgagg ctgcaaatca
    13561 taccctgact tttgccgggt atacagtgtc aggacatttt tttgtaactt gtgtctgtcg
    13621 gaaccacatg aagaacctgc tgataatatt agccctcgtt gccttcctgc catcagacca
    13681 ggaagaggtt cgctacactc aggcccgaag ccggatggtg aagagccaga tagctgcacg
    13741 cggcatctct gaccctgcca cgctgagagc catggagcgg gtcccgcgcc acctgtttgt
    13801 acctgcagcc ctcgcaggtt cagcatacag tgaccagcca ctgccgatag gttacaacca
    13861 gaccatatca caaccgttca ttgttgccta tatgactgaa ctgtcaaagc cggccaaagg
    13921 taaaaaagcc ctggagatag gtacaggctc ggggtaccag gctgccgtac tggccgagat
    13981 tgttgatacg gtctatacca ttgagattgt gccggagctg gccagggaag ctgcttcgcg
    14041 gcttaaggta ctgggttacg acaatatagt tgtcaggtac ggcgacgggt acaaggggtg
    14101 gcctgaacac tctccctttg atataatact ggtgactgcc gcagctgacc atatacccga
    14161 accacttaag gaacagctgg cagagggagg acggctggtg atgccggtag gaaatcctgc
    14221 gacatttcag gagcttattg tgctgaccag aagaaagggc aggataaccg aacagcggct
    14281 tgctccggta cggtttgttc ctctgaaaag attaaaatag ctatttttac aagccccgca
    14341 cactgaccgg gctgaggaat gctgaacctg ttaatacacc tgcaatgata tacgtaatta
    14401 tcgcggcagc agttatattg ctgctttcgg cattactgat cttcaactcc cttatccgga
    14461 aaaagaacga cgtggagaat gcatttgcct ccattgacgt tatgctcagg aaaaggtatg
    14521 atcttatccc acagatcgtt gaaaccgtaa aggcttacat gaattatgaa agagagctcc
    14581 tcaccaaaat tactgaattg aggatcaggg caatgtcaca aacgctaacg gcagaggaga
    14641 gggtgcaggt ggaaaaccgc ctcggtacca gtctgactga tctgctgatt gcagtggaga
    14701 attaccctga tctcaaggcc agcaccagct tcctgcagtt gcaggctacc ctcaacgagg
    14761 tggaggagca gcttgcagca tcacgccgtg cattcaatgc tgccgttaca gtatataaca
    14821 acagcattga ggttttccct tcgaacatta ttgcctccat gatgcgatat aagagaagat
    14881 ctctctttga gatccccgca gcgaaacggg aagaggtcag ctccataccg cccgttaaca
    14941 tggtataaag cataatacca gaatgagcag atacggcacc ttcagggagt tttatgaaaa
    15001 ggaactgctg cctgaccttg agtcaattga tatgaggcgc agggcaacca ataaaaaggt
    15061 cctgaccgtc ctgtttgtta cagccgctgc cgtgctggcg gagctgttgc ttatacctga
    15121 caacacggat ttcccaaagt cggcaccgat gattattacc gccttcgcgg gactggttgc
    15181 tgccggaatt gtcagcaaaa actacaggaa ggagtacaag gaaaagataa tagcacgtat
    15241 cgcaggatat gcagaggaag ggcttacata taagcctgat gcatccattg ccaggtatga
    15301 tttcgtgaaa agcaggattt ttacactctc atgcgacagg tacagcggag aggattattt
    15361 tcgcggcagg ataggcaaga ctgaaacaga gttttctgaa gtccacgcca tgcaccggaa
    15421 taccagcggt acgggcagcc agcagaggga taattatact accatattca aaggcatatt
    15481 cattatcgca gatttcaaca agaatttcag aacacatact gttgtgcttc cggacactgc
    15541 cgaaaagctc ttcggcaaat tcggacagtc gctccagtct gtttcattcg gcaggggtaa
    15601 actgatacgc ctggaagacc cccgctttga aaaggagttc tgcgtttacg gtgatgacca
    15661 gatagagtca aggtatatcc tcaccccttc cctgatggaa cggatacttg ccttcaggga
    15721 gaagtggaag acaaaagtgt acctctcttt cctcgactca aaggtctata tagccataag
    15781 catgaacagg gacctcttcg agctccggcc tttcaagcct gcagccgact ttgacttcct
    15841 tgaagagact ctccggttcc tgacactgct taccggggtg gttgatgatc tcaacctcaa
    15901 tacccgtatc tggaccaaag aataacctca ctctcaaccg tacctgaagg gcatcatcat
    15961 gaaaaggcag acacattttt atggaatgat agtcccgctt tcagagatga ccattgcaga
    16021 tttgctacct ttgtctttat ggaaaaacta aacctctttt ttgaccggat aaaggatctc
    16081 agcttctggc agaggctctt ccaatggaga agagtccgga acctcagctt tgatgcctac
    16141 gaggagttcc gtgcactcgc cagggagaca gaggctgtca gggagaccgc cggcaggctg
    16201 cagaaccgca tcacggaact gtcggcacac aacgaaaacc tctcccggag ggtgcatgag
    16261 atggaggtgc agtcggcacg caaggatgca aatatcgagg agctcaacgg gcgccttcgc
    16321 gaagactcag ccagcataca ggaactcaca aaaaaagtgg caggtttcga agccacacgt
    16381 gaagacaacg ttaaaaacta taacgagaac atcgcacggc tgaaccagac caaggagagc
    16441 ctcgaggctg aacggcgcag gctgaac
//
LOCUS       DAOPBK010000040        16383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3600_length_16383_cov_4.597562, whole genome shotgun sequence.
ACCESSION   DAOPBK010000040 DAOPBK010000000
VERSION     DAOPBK010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16383
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_16383_cov_4.597562"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLA17_03825"
     CDS             complement(<1..417)
                     /locus_tag="PLA17_03825"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HPK84573.1"
                     /translation="MMKKGVKITVIILVTLLLTVMAAAIIIPVVFKDKIKEKVENVLN
                     EKLTAKVSFDNYRLNLFRAFPNASFSLVDLSVTGTGDFEGDTLASVKSAGIVINLRSL
                     FGDGGYEIKSVIIDKPFVNAIINNAGKANWDIVREFP"
     gene            complement(742..2223)
                     /locus_tag="PLA17_03830"
     CDS             complement(742..2223)
                     /locus_tag="PLA17_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPK84574.1"
                     /translation="MKRFIILFLITVFVSACNRGGQQYDAAPETPDTGDLTMGAGLQK
                     FFSLRREAILDTIGDGLLVLRSDYGFNGGRHGYRAASNFWYLTGYGQPGTIMSLSGGD
                     SRPYALYTRERSVRDIIYSGNLPDPRELIGTWAPDTLLDYREADKIIAKAARAGRHIY
                     LDFRDRVFRDHVLELLGAGGNAGALLRDINPALSEMRVDKDEYEVMMLQKAIDITGKG
                     FETVLAECAPGMYEFEIEALLEYEWRRNGSPMPAFGSIVGSGENAVTLHYSANNRKME
                     EGDLLLMDVGAEYGYYAADITRTIPVSGRFTEEQRDIYDLVLRAQKAAIGEMKPGAPI
                     TAAHRIATNTIIDGLHALGLVTDPDCQWQRKFYTIYHICHFLGLDVHDTGNQGIPQSM
                     IGSYLTADTAVGRPLQAGMVLTVEPGIYLRANGLDQLELLYGREAGEGEIADFIGKVR
                     PVYERYSNIGVRIEDDVLITATGNTVLSASIPKEPNEIERLMK"
     gene            2334..3209
                     /locus_tag="PLA17_03835"
     CDS             2334..3209
                     /locus_tag="PLA17_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPK84575.1"
                     /translation="MHRTAIIFTLISIMILSFRSDKPAYKLYDSEGKIVKYEKMLNRL
                     SSADVVFFGELHDNPIAHWLEYEVTAGLYEIIRDRLVLGAEMFETDNQLLIDEYLRSE
                     YEADKFEAEVKLWKNYRTDYRPLVEFARKNNLPFIATNVPRRYASMVSRGGFEELDSL
                     TAEAKRYLAPLPVSYDPELKCYKDMLSMQGMPGMGARPNQNLPKAQAVKDATMAHFIA
                     ENLRPGKVFIHYNGAYHSDNYLGIVHYLKLYRPEVKVATITTVLQEDITDLEEENRGL
                     ADFIVAVPVTMTRTY"
     gene            3258..3881
                     /locus_tag="PLA17_03840"
     CDS             3258..3881
                     /locus_tag="PLA17_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPK84576.1"
                     /translation="MQTRVRQILDQEAEAVRKIPVTESYNQAIALIHKHVHLLKGKLV
                     TSGMGKAGQIAQNIATTFSSTGTPAVFLHPSEARHGDLGVLHENDIMLVISNSGRTRE
                     ILELIELKNNLFPDMPVVVITGNNSSPLWKGASVGLYTGGPAEVCPLGLTPTTSTTVM
                     TVIGDVLVVLMIEKTGFTAAEYAKRHHGGYLGYKSRECSGNSGETIY"
     gene            complement(3875..4669)
                     /locus_tag="PLA17_03845"
     CDS             complement(3875..4669)
                     /locus_tag="PLA17_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPK84577.1"
                     /translation="MMKKSEIIERIKSKKAFISDMDGVIYHGNRLLPGVPEFVEWLKK
                     EGKKFLFLTNSSERTPKELQEKMLRLGIDIDENAFYTSALATAQFLASQKPEGTAYII
                     GEAGLINAMYNAGYTMNNYNPDYVVVGDTRSYSFEKIEQAVNLVLKGAKLIGTNPDLT
                     GPVENGIIPATKALISPIELSTGKQAYFVGKPNPLMMRIALKKIGCSNEETIIIGDRM
                     DTDIIAGIESEIDTLLVLSGISTLKTAEKFAYRPSYILEGVSELVQ"
     gene            4963..6279
                     /locus_tag="PLA17_03850"
     CDS             4963..6279
                     /locus_tag="PLA17_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HPK84578.1"
                     /translation="MSRKNYIVGMTVIAIFFFIFGFITWLNGILIPFLHTACELTEFQ
                     AYFVTTAFYISYFVMSLPSSEVLKKTGFKNGMSIGLWVMAVGALIFIPAALSRSYAIF
                     LLGLFVQGTGMALLQTASNPYVTIIGPRESAAKRISMMGIANKFAGAIAPIILGAHIM
                     KDSHVLDRQLAEAADAVMRAGILDQLAARVINPYIIMAVILVALGLLLRFAHLPEVDT
                     DSEEPTVGESNVNKTSIWHFPHMILGAIALFFYVGVEVIAGDTIIRYGQSLQIDMSSA
                     KYYTSLTMLGMIIGYICGIIFIPRMLSQVNALRISAVLGIIFTFGAVLTPAGQQITMP
                     FRDLVTFQAVTLTIPVTVLFVALLGLANALVWPSVWPLALNGVGRFTKTASALLVMAV
                     VGGAIIPLIYGKLADIFNTQSAYWVCLPCYLILLYYSLHGYRLKGR"
     gene            6289..7389
                     /locus_tag="PLA17_03855"
     CDS             6289..7389
                     /locus_tag="PLA17_03855"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPK84579.1"
                     /translation="MGTDLKSIFSRFRGEGTFLEGKSFGPGHIHDTFRIITAEQECDN
                     YILQRLNSNVFRDIPGLQENMERVTGHLRNKIAAIPGSDLKRECLTLVPDRETGRSFI
                     TDSEGRFWRMFIFISDHRSYEKVDSEHKAFEGGRIIGRFQAMLADLPGKPLHETIPDF
                     HNIASRLDAFHRVISLDPAGRVASVRSEIAEITSREEEMKTIIRLGAAGKIPIRITHN
                     DTKFNNILFDRDDRALLIIDLDTVMPGYVHYDFGDAIRTAANRADEDAEDLSTVSMDI
                     NIYRAYAEGFLSETRNTLNAVEIEYLPFAPKLLTYIMATRFLTDFIDGDNYYKIRHPL
                     HNLQRTRAQIALLRSMEAQYGDMVQIIKNITK"
     gene            complement(7549..9006)
                     /locus_tag="PLA17_03860"
     CDS             complement(7549..9006)
                     /locus_tag="PLA17_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPK84580.1"
                     /translation="MSEIKKLEPKLLWHYFYEITQVPHPSKKEHRMTEFLKKFGEEHG
                     LETIVDKTGNVIFRKPATKGLENRKGIILQAHVDMVPQKNTDKKFDFEKDPIETWIDK
                     GWVKAKGTTLGADNGIGVAAAMAAMTDPKLAHGPLEALLTVDEESGMTGARNLKKGIL
                     KGEILLNLDSEDEGELYVGCAGGVDVGATRNYRQEKTPEGMTAYKLSLKGLRGGHSGL
                     DIHLGKANANKLMFRFFQHAEHDFGMRIADYTGGDLRNAIPRESFATIVVPSIKKAQF
                     EKFMKGYEKMYVAEFKEADPDIKLIFKVADAPAKVMNEADQYRIIRGVFVCPNGVQRM
                     STSMPGVVETSNNLSIVRIGNGKFEAYCLCRSSVDSAKEATAWKIAAGFQLAGCEVEL
                     SGSYPGWKPDMNSAILSTMKDTYNKLYGRIPEIKAIHAGLECGIIMGVYPKLDAISFG
                     PTIRHPHSPDEKVEIESVVKFWNFLVTTLKNAPKA"
     gene            complement(9014..10429)
                     /locus_tag="PLA17_03865"
     CDS             complement(9014..10429)
                     /locus_tag="PLA17_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK84581.1"
                     /translation="METIRKTLADSKTARWTALAVVAFTMLCGYFLTDVMAPLKPLLE
                     QQLSWSSEEYGLFTSAYGWFNVFLLMLIIGGIILDKMGVRFTGKMATMLMVAGTAVKY
                     WAVSTDIFAGQTWHFLFWDANPQVLIAGLGYAIFGVGVETAGITVSKIIVKWFKGKEL
                     ALAMGLEMASARIGTGLALAGSAPLAKALGHVSMPVLVALILLCVGMIAFFIYCGMDK
                     KLEASIAQSKEGEPEEEQEESFKLSDILFIIKNRGWWFIAILCVLFYSAVFPFLKYAA
                     DLMVNKFGMDPATAGLIPSLMPFGTILLTPIFGNLYDRRGKGATIMIIGALLLIFVHS
                     MFSIPAVNKPWMALLLIIVLGIGFSLVPSAMWPSVPKIIPYQKLGTAYALIFWVQNWG
                     LMGVPYLIGWVLDNYCITGTRILEGVEVPTYDYTLPMMIFTAFGLLAIVFGFLLKAEN
                     KRKGYGLEMPNIEEKKVTTDN"
     gene            10600..11121
                     /locus_tag="PLA17_03870"
     CDS             10600..11121
                     /locus_tag="PLA17_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HPK84582.1"
                     /translation="MKELIFVFLLAAAGLTVSAQESAVKWYSIEEAVALAQKNPRPIF
                     VDTYTDWCGWCKKLDKDTFSNPVIAEILNNKYYAVKFDAEGKEPVVFQGRKFVNDGSL
                     GKTHQLAYALLQGKLGYPTVVFLTASSELITPVSGYKTPEQIEPLLNYFAGTGWQKQS
                     YDDFLKTFTPKVR"
     gene            11168..11881
                     /locus_tag="PLA17_03875"
     CDS             11168..11881
                     /locus_tag="PLA17_03875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK84583.1"
                     /translation="METREEIGKWYDGFADCQEKTGVNLRHYKIIGFLTGAGLSRHSR
                     VLEIGCGIGTLTGLLSRYLRKGQIVAVDISSRSVEIAKKRLKHAGNVMLMVSDMTDFH
                     DPGLFDFIVLPDVLEHIPASQHRSLFSTLASHMHDNSVILIHIPHPRAIDYLRKTSPD
                     KLQIIDQAIEADALLADAAASDLVLVNYISYSLFNKENDYAVITLRKKCAVTLAPLSA
                     IIISARKMSARIRFLLSRL"
     gene            11891..12961
                     /locus_tag="PLA17_03880"
     CDS             11891..12961
                     /locus_tag="PLA17_03880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK84584.1"
                     /translation="MNRKILYIYPWEAAFIDKDLDFLSRRYSVVTLGHDWRDKRRTLP
                     NFLKQFIFLCRNMAGTRAILVMFGGYWSFLPALAGKFTGVPVYIIPGGTDCVSFPSLN
                     YGSLRKPVMRTFIRWSYQLCTKLLPVDESLVLSDYTYLPDADHPKQGFRYFFPGLLTP
                     HRVIHNGFDPAFFTADPAGKKENSFIAVAHAQEMQRVRIKGIDLVILLAKRFTNCTFT
                     LVGVSDSLKRELSPLPSNLSVYPWMSKEKFIGHLAESRFVLQLSVSEGFPNALCEAML
                     CHCIPVGSAVGAIPSIIADTGFVIASPEWEYLEKRFEEILHTGAEEQHRLGIMARNRV
                     ADMYHISRREKAFLEILDEIPG"
     gene            complement(12984..14105)
                     /locus_tag="PLA17_03885"
     CDS             complement(12984..14105)
                     /locus_tag="PLA17_03885"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HPK84585.1"
                     /translation="MKKESIIVLALLALMISGCSPYSGKTPADTVVSQLGDKISVTDG
                     CLVYALPMTVFELDIIAEKRTEVPGPYARYASELTGLDNVITGHTEKWSLAGIRLSAV
                     EELDPSQFYIIQGTAMMQTNMLALRKSGLVLDINPDLYSSATYSNLQGDPDYAGMLFP
                     DRGAYEYVVTKTDTAYRLVKVDTAFIRVPYLVQKKKGMSLVEEAREAAGRLLELREGR
                     HMILTGETNVFPQDGAALEEINRLEREYMALFAGKSFTETRHFRIWITPDQQMAGQKT
                     TIFTFSETSGVNTSPDGPGEPVLMEIGPSGKTRDLNMVMRPASMQKHANPADRVYYRV
                     PDVAEINISLAGENLCTARRLIYQYGSLVALPANFIIGK"
     gene            complement(14114..15643)
                     /locus_tag="PLA17_03890"
     CDS             complement(14114..15643)
                     /locus_tag="PLA17_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPK84586.1"
                     /translation="MKIFTSAEIREIEAATIRLEPVTPAGLMERAATALFQQVKDIVA
                     PERKINVFAGPGNNGGDGLVLARLLHEDGYVVRVFVIETGSARSSELNLNAERLERSG
                     IIPVTITDPGQVPAIWRDEVIIDAIFGTGLRKSPSGVAAGVIQRINETGAFVISVDVP
                     SGLFCEDNQDSDRDSIVRASKTLTFQFPKLSFMFAGNGIYTGEWEVIDIGLHTDTISS
                     MATPCHYVLRETVRPMIRKRNKFDHKGTYGHALLIAGSYGKAGAATLSAGAALRSGAG
                     LVTVHTPSSAVLALQAALPEAMVSPDSGTGNVTALPDLKPYNAIGVGPGLGTDPDTAS
                     VLRELLKNISVPLILDADALNIIAQNREWLDLLPPLTVLTPHPGEFRRLTGTTSTDFR
                     LVQEQREFAVRYRCVVVLKGAHTSVALPDGTLFFNSTGNPGMATGGSGDVLTGIITSL
                     LAQGYDPSDAAVAGVYLHGLSGDIALRIHSEEAVIAGDILMNIGRAFRETNFSNFNVY
                     L"
     gene            15792..>16383
                     /locus_tag="PLA17_03895"
     CDS             15792..>16383
                     /locus_tag="PLA17_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK84587.1"
                     /translation="MLKNHPKGLLVAFFANMGERFGFYTMMAILVLFLQAKYGLAAEK
                     AGDIYSWFYFAIYALALVGGILADSTRKYKTVILFGIIIMFAGYILMAVPGMSLTFVV
                     IALFTIAFGNGLFKGNLQAVVGQMYDDPKYSKLRDSAFMIFYMGINIGAFFAPYAANG
                     IRNWWLKTNGFAHDGSLPALCHQFTGGQMTDPAIVQE"
BASE COUNT         4119 a         4270 c         3772 g         4222 t
ORIGIN      
        1 cgggaattcc ctgacaatat cccaattggc tttcccggca ttgttaataa tggcattgac
       61 aaatggttta tcaatgatga cagacttgat ttcataaccg ccgtcaccga agagactcct
      121 gaggtttatt actattcctg ccgacttcac tgaggcgagg gtatctccct cgaagtcgcc
      181 ggttccggtc acactcaggt caacaagaga gaaggaggcg ttgggaaagg ccctgaacag
      241 gttcagcctg taattgtcaa aagagacttt tgcagtgagt ttctcattca gaacgttttc
      301 aaccttctcc tttattttgt ccttaaaaac tacaggaatt atgattgccg ctgccattac
      361 tgtcagcagc agtgtcacga gtatgattac agtaatttta actccttttt tcatcatagt
      421 atagttttga ttttaagagt ttgatgtctg tccagctcaa gcaggggaac ccggatgact
      481 gaaaattgga taaacaggtg tttctgtatt tgatacatat tgggttcacg ccagtcataa
      541 ctgatattgt tgtttaacgg aaaagctgtc agaatagttc tgtgtgtatg aaagaacatt
      601 acaaaatgca ggccggtcac aaaagtaggc aaaattgcaa ttgcactgac atcccgggca
      661 aataagcaac aggctgcttc acaagcaggt gataaccacc agcatcttca ggtactgtac
      721 aataagctaa taagcaacag gctacttcat aagcctctct atttcattgg gctccttcgg
      781 gatggatgcc gaaagtacag tgttgcctgt ggcagtgata agcacatcat cctctatcct
      841 gacacctata ttactgtatc tttcgtaaac gggcctcact ttcccgatga aatcggcaat
      901 ctccccctct ccggcttctc tgccataaag caactcaagc tgatcaagcc cgtttgcccg
      961 gagatagatg ccaggttcca ccgtaagcac catccctgct tgaagaggtc ttcccactgc
     1021 cgtatcagct gtgagataac tgccaatcat tgactgtggt attccctggt ttccggtatc
     1081 atgtacgtca agtcccagaa aatggcagat gtgatagata gtatagaatt tgcgctgcca
     1141 ctggcagtca ggatccgtta ccagccccag agcatggaga ccgtcaataa tagtgttggt
     1201 tgcgattctg tgtgcagcgg tgatcggcgc tcccggcttc atctcaccta tcgcggcctt
     1261 ctgtgcccgc aataccagat cataaatgtc cctctgctcc tctgtgaacc ttccgctcac
     1321 gggaatggta cgggtaatgt cagcagcgta gtatccgtat tcagcaccaa catccatcag
     1381 caacaggtca ccctcctcca tcttcctgtt gttagccgaa taatgaaggg taacggcatt
     1441 ctcacctgaa ccaactatag acccgaaagc aggcatcggg gatccgttcc gccgccactc
     1501 atattccaga agggcctcta tttcaaactc atacattccc ggggcgcact ctgccagaac
     1561 tgtctcaaaa cccttgccgg taatgtcaat cgccttctgc agcatcatta cctcatattc
     1621 atccttgtcg accctcatct ctgacagcgc cggattgata tccctgagta atgcaccggc
     1681 atttcccccg gctcccagca gctccagcac atgatccctg aaaacgcggt cgcgaaagtc
     1741 aagatagatg tgccttcccg cccttgccgc tttggcaatt atcttatcag cctcacgata
     1801 gtcaagcagg gtatcaggag cccatgttcc tataagttca cgagggtcag ggagattccc
     1861 tgaatagatg atatcacgaa cactcctctc ccttgtataa agggcataag ggcgggaatc
     1921 cccgcctgac agagacataa tggtccccgg ctgcccgtat cccgtcaggt accagaaatt
     1981 gcttgctgcc ctgtatccat gcctgcctcc gttaaatcca tagtctgacc ggagtacaag
     2041 cagaccgtca ccgatagtat ctagtatagc ttcccgcctg agagaaaaga atttctgaag
     2101 cccggcaccc atggtcaggt cacctgtatc aggtgtttca ggagccgcat catactgctg
     2161 cccgccacgg ttgcaggcag acacgaaaac agtgataaga aagagaatga taaacctttt
     2221 catggaacaa atgttgtatc cccaaagata ttacaaatca atccggaata attggtttaa
     2281 tttgctttca attgctaata aatcttaatt atcaaccctt acaatccaca ataatgcaca
     2341 gaacagccat catattcacc ctgatctcca taatgatcct ctccttcagg tcagacaagc
     2401 ctgcatataa actgtatgat tctgagggca agatagttaa atatgaaaag atgcttaaca
     2461 ggctcagctc ggcagacgtc gtctttttcg gggaactgca cgataatccc attgcccact
     2521 ggcttgaata tgaggtcact gcaggtctct atgaaattat tagagataga cttgtgctgg
     2581 gcgccgagat gttcgagacc gacaaccagt tattgattga tgaatacctc cgatcagaat
     2641 atgaagctga caagttcgag gctgaggtta agctctggaa gaattacagg acagattaca
     2701 gaccgctggt tgagtttgca aggaagaaca acctgccctt cattgcaacc aacgtgccgc
     2761 gcaggtatgc ctcaatggta tcacgtggcg gatttgagga gcttgactca cttactgctg
     2821 aggcgaagag gtatcttgca ccgttgccgg tttcatatga tcctgagctg aagtgctaca
     2881 aggatatgct ctcaatgcag ggcatgcccg ggatgggtgc caggccaaac cagaacctcc
     2941 cgaaggccca ggctgtcaag gatgccacaa tggctcactt cattgcggaa aacctgcggc
     3001 cgggaaaggt cttcatacat tacaacggag cataccactc tgacaactac ctggggatag
     3061 tccactacct gaagctttac aggccggaag taaaagttgc aaccattaca accgtactgc
     3121 aggaggatat aactgacctt gaagaggaaa acaggggtct ggctgatttc attgtagctg
     3181 tgcctgtgac catgacacgg acatactgaa acagaataag tttaaaataa atatctttgg
     3241 attcttattc cagaatcatg cagaccagag tcagacagat acttgaccag gaggctgaag
     3301 ctgtcaggaa gatacctgtc acagagagct acaaccaggc aattgcactg atacacaaac
     3361 atgtacacct gcttaaaggc aaactggtaa caagcggtat gggtaaagcg ggacagattg
     3421 cccaaaacat tgccactaca ttcagctcca ccgggacacc ggcggttttc ctccacccca
     3481 gtgaagcccg gcacggtgat cttggcgtgc tccatgaaaa tgacattatg ctggtcatct
     3541 ccaattcagg acgcacccgg gagattctcg agctcattga actgaaaaac aatctcttcc
     3601 cggacatgcc tgttgtggta atcacaggca ataatagttc gccactatgg aaaggggcca
     3661 gtgtgggcct ttatacaggc ggccctgccg aggtgtgtcc gcttggactg acacccacca
     3721 cctcaaccac tgtcatgacc gtcatcggtg acgtgctggt ggtccttatg attgaaaaga
     3781 ccggcttcac cgctgcagaa tatgccaaac ggcaccatgg cggatacctg ggttacaaat
     3841 caagggaatg ctccggaaac tccggagaga ctatctactg aaccagttca gacactccct
     3901 caagtatgta tgagggtctg taggcaaact tttcagccgt cttcagggtt gagatgcccg
     3961 acagtaccag cagggtgtct atctctgact caatgccggc aatgatatcc gtatccatcc
     4021 tgtcgcctat gatgattgtc tcctcatttg aacacccgat ttttttcagg gctatcctca
     4081 tcatcaaggg attaggcttg ccaacgaagt atgcctgctt gccggtggag agctctatcg
     4141 gtgagatgag agccttggtg gccggaataa tgccgttctc taccggacct gtcaggtcag
     4201 gatttgttcc gatcagcttg gcgcccttaa gcaccaggtt gacagcctgt tctatctttt
     4261 cgaaactgta gctgcgggta tcaccaacaa ccacataatc aggattatag ttattcattg
     4321 tatatcctgc attgtacatt gcattgatca gaccggcctc tcctattata taagccgttc
     4381 cttccggttt ctgtgaggcc aggaactggg ctgtggccag cgcactggta tagaaagcat
     4441 tctcatcaat atctatcccg agcctgagca tcttttcctg tagctccttg ggggttcttt
     4501 cactgctatt ggtcaggaat agaaacttct ttccctcctt cttaagccac tccacaaatt
     4561 ccggaacgcc cggaagaagt ctgttaccat ggtatatgac accatccatg tcactgataa
     4621 aggccttctt gctctttatc ctttcaataa tctcactctt tttcatcatt ctgatttctg
     4681 gtgcaaaatt aatacttcgt tgccatattg cattataata agttgtgtgg tcttaaaaag
     4741 agagacatgg cgggtctttt cttatccggc cagactgcat attggtcagg agagcctcaa
     4801 acaggaagga attgccataa attaaaagtg atattgcagt ttatttccaa aaagcgtagt
     4861 tttgatgcct gaccgacgca aatttcactg cagcggcaaa actaaccaca acttattaat
     4921 cataatataa acaagatgac ccaaggtgca acatcggccc ccatgagccg caagaactac
     4981 atcgttggca tgacggtaat tgccatattc tttttcattt ttggttttat tacctggctc
     5041 aacggaatac tcattccatt tctgcacacg gcctgtgagc tgactgaatt ccaggcatat
     5101 tttgtcacca cagcttttta catctcatac tttgtgatgt cactgccatc atctgaggtg
     5161 ctgaagaaaa ccggcttcaa aaacggcatg tcaataggcc tctgggttat ggccgtgggt
     5221 gcactgattt tcattcctgc tgcgctttcg cgaagctatg ccatcttcct ccttggcctc
     5281 ttcgtccagg gtacagggat ggccctgctg cagaccgcct ccaacccgta tgtgactatc
     5341 ataggtccgc gcgagagtgc tgccaagcgg atcagcatga tggggatcgc caacaagttc
     5401 gccggcgcaa tagccccgat catcctcggt gcacatatta tgaaggattc ccatgtgctt
     5461 gacaggcaac tggccgaagc cgccgatgca gtcatgcggg caggcatcct tgaccagctt
     5521 gccgccaggg taataaatcc atatataata atggctgtca tcctcgtggc gctcggactg
     5581 ctccttcgct ttgcgcacct gccagaggtg gataccgatt ctgaagaacc cactgttggt
     5641 gaatccaacg tcaataaaac cagtatatgg catttcccgc atatgattct tggagccatc
     5701 gcactcttct tctatgttgg cgttgaggtc atcgccggtg atactataat aagatatgga
     5761 cagtcattgc agattgacat gtcatcagca aaatactaca catcgcttac catgctggga
     5821 atgatcatcg ggtatatctg tggtatcatc ttcataccca gaatgctttc acaggtaaat
     5881 gccctgcgca tatccgccgt ccttggaatt atcttcacct tcggcgctgt tctcacccct
     5941 gcagggcagc agatcaccat gcccttcagg gatcttgtaa ccttccaggc tgtcacgctc
     6001 acaatcccgg ttactgtgtt gtttgttgca cttctcggac tggccaatgc acttgtctgg
     6061 ccctctgtgt ggccactggc ccttaacggt gtgggccgtt tcaccaaaac agcctcagcg
     6121 ctgcttgtga tggccgtggt aggaggcgca atcatacctc tcatctacgg caaactggcc
     6181 gacatcttca acacccagtc tgcttactgg gtctgcctgc cctgctacct tatcctgctc
     6241 tactactcac tgcacggata caggctgaag ggaagataga gagccactat gggaaccgat
     6301 cttaaaagca tattcagccg cttccgcggc gaaggtacat tccttgaagg aaagtctttc
     6361 ggcccgggac atatacatga caccttccgg atcataaccg ctgagcagga gtgtgacaac
     6421 tacattctcc agcggcttaa cagcaacgtc ttccgtgaca ttccaggcct ccaggagaac
     6481 atggagaggg ttaccggcca cctgaggaat aaaattgcag ccattcccgg gagcgacctg
     6541 aaacgggagt gtcttaccct ggttcctgac agggagacag gaaggtcttt catcactgac
     6601 agtgagggcc gattctggag aatgttcata ttcatctctg accacaggtc atatgaaaag
     6661 gttgactccg aacataaagc ctttgaaggt ggaagaatca taggaaggtt ccaggccatg
     6721 ctggccgatc tgcccggaaa gcctctgcat gaaacaatac ctgattttca taatattgcc
     6781 agccgccttg atgcctttca cagggtaatt agtctggatc cggccggcag ggtggcatcg
     6841 gtcagatcag agattgcaga gatcaccagc cgggaggagg agatgaaaac catcatcaga
     6901 ctgggtgccg ccggaaaaat tccgatcagg ataacccata atgataccaa gttcaacaat
     6961 atcctcttcg acagagatga cagggcgctg ctgataatcg atcttgatac ggtgatgccg
     7021 ggatatgtcc attacgactt cggagatgcc atccgcacag ccgccaaccg ggctgatgaa
     7081 gatgcagaag acctttcgac cgtatcaatg gatatcaaca tctaccgtgc atacgccgaa
     7141 ggtttccttt cggaaacaag aaatacgctc aatgccgttg aaatagaata tcttcccttc
     7201 gcgccgaagc tgctcaccta cataatggca acacgtttcc tgactgactt tattgacggc
     7261 gacaactact acaagatcag gcatcccctc cataacctgc agcgtacaag ggcccagatc
     7321 gcccttctcc gcagcatgga ggcccagtac ggggatatgg ttcagataat caaaaacata
     7381 acaaaataaa gaaattcatc acacacggaa gtatacgtcc agagtgttct ccctgtcaac
     7441 ccggccaccg ggttgtcaaa gggccacacc aacaaaactc ctcagttcat ggagctgaat
     7501 agtcttctgc tttaaaagaa ctatcgtatt ccgtgcagtg tcagcatatc aagcctttgg
     7561 tgcattcttg agagttgtca ccaggaaatt ccagaacttg acaaccgatt cgatttccac
     7621 cttctcatca ggactgtgcg ggtgacggat ggtcgggcca aagctgattg cgtcaagctt
     7681 cggataaaca cccatgatta tgccgcactc gagacctgca tgaattgctt ttatctcggg
     7741 tatcctgcca taaagcttat tgtaggtatc tttcatggta ctgaggatgg cgctgttcat
     7801 gtcaggtttc cagcccggat agcttcccga tagttcaacc tcacagcctg ccagctgaaa
     7861 tcctgcagca atcttccatg ctgttgcttc cttggctgaa tcaacggagc ttctgcagag
     7921 acagtaagcc tcaaacttgc catttcctat cctgacaatg gagagattgt ttgatgtctc
     7981 aaccacgccc ggcatactgg tgctcatcct ctgaacgccg ttggggcaaa caaatactcc
     8041 ccttatgatg cggtactggt ctgcttcatt catcaccttc gcgggcgcat cggcaacctt
     8101 gaagatcagc ttgatatcag gatcagcctc cttgaattca gctacataca tcttttcata
     8161 acccttcatg aacttctcga actgggcttt tttaatggag ggaacaacta ttgtggcaaa
     8221 tgactccctg gggatggcat ttcgcaggtc accgcctgta tagtcggcaa tacgcattcc
     8281 aaagtcgtgc tcagcatgtt gaaaaaatct gaacatcagc ttattggcat tggctttccc
     8341 aaggtgaata tcaagtcctg aatgccctcc cctgagtccc ttcaggctca gtttataggc
     8401 cgtcatcccc tccggagtct tctcctgcct gtagttgcgt gtggcaccca cgtccactcc
     8461 gcctgcacag ccaacgtaaa gttcaccttc atcctccgag tccagattca gaagtatctc
     8521 tcctttgagt atgccctttt taaggttcct ggcaccagtc atgccggatt cttcatcaac
     8581 cgtgagcagc gcctccagag ggccgtgagc aagcttggga tcggtcatcg ccgccatggc
     8641 ggcagccact cctataccat tgtcagcgcc aagcgtggtg ccttttgcct taacccatcc
     8701 cttgtcaatc caggtttcaa tgggatcttt ctcaaagtca aattttttgt cggtattctt
     8761 ctggggcacc atgtcaacat gagcctgaag aattattccc ttgcggttct ccagcccttt
     8821 ggtggccggt tttctgaaga tcacgttccc ggttttgtca acaatggtct caagaccgtg
     8881 ttcctctccg aactttttca agaattccgt catccggtgc tccttcttgg agggatgcgg
     8941 cacctgtgtt atttcataga aatagtgcca caggagcttt ggttcaagtt ttttaatctc
     9001 actcatggtt atcttagtta tctgttgtta ctttcttttc ttcaatattg ggcatctcca
     9061 gaccatagcc ctttctcttg ttctcagcct tcagcaggaa gccgaacact attgccagta
     9121 agccgaaggc agtaaagatc atcattggta atgtgtagtc ataagtgggc acttcaactc
     9181 cttccaatat gcgggtaccg gtaatgcaat agttgtcgag gacccatccg atgaggtaag
     9241 gcactcccat gagtccccag ttctgaaccc agaagatcag ggcatatgcg gtaccaagtt
     9301 tctggtaggg aataattttg ggtactgaag gccacattgc tgaaggtacc agagagaaac
     9361 ctatacccaa tactataatc agcaataatg ccatccatgg cttattaaca gccgggatgg
     9421 aaaacattga atgtacaaaa atgaggagaa gggcgcctat tatcattatt gtagcaccct
     9481 tgcccctgcg gtcatacagg tttccgaaaa tgggtgtcag gaggatggta ccaaacggca
     9541 tcaatgaagg tataagtcct gctgttgccg ggtccattcc aaacttgttc accataaggt
     9601 ctgctgcata tttgaggaac gggaacaccg ccgagtagaa gaggacgcat aatatggcaa
     9661 tgaaccacca tcccctgttt ttgattatga acaatatatc tgaaagcttg aagctctcct
     9721 cctgctcttc ttccggttcc ccttcctttg actgcgctat ggatgcttca agcttcttgt
     9781 ccattccgca gtagataaag aaggctatca tgcccacgca cagcaggatg agtgcaacca
     9841 gaaccggcat ggaaacatgt ccgagggctt tggccagggg tgctgatcct gcaagcgcca
     9901 atccggtacc tattcttgca ctggccatct caagccccat tgcaagagca agttctttgc
     9961 ccttgaacca cttcacaata attttcgaaa cagttatacc ggcagtctca acaccaactc
    10021 cgaatattgc ataaccgagc cctgcaataa gaacctgcgg gtttgcatcc cagaagagaa
    10081 aatgccatgt ctggcctgca aaaatatcgg tagagacagc ccagtacttg acagccgtac
    10141 ctgccaccat aagcatcgtt gccatttttc ctgtaaacct cactcccatc ttgtcaagaa
    10201 tgatacctcc gataatcagc atgagaagga atacattgaa ccagccataa gcactggtga
    10261 acaacccgta ctcttcactg ctccacgaca gctgctgctc aagcagcggc ttgagaggcg
    10321 ccatgacatc agtcaggaaa tatccgcaca acatggtgaa tgctacaact gccagggcag
    10381 tccatcttgc tgtctttgag tcagcaaggg tctttctgat cgtttccatt ttaagatttc
    10441 gtgtttaaca ttgtgaacat ggtgcgtaaa tgtataaaaa attagttcca attattgcgt
    10501 taaaaaacat agaactgaac tttgtgccaa ttggactgaa agactaattt tgtgcaatca
    10561 tcgttaaatt gttcaaccgg atataattaa tgatctaata tgaaggaact gatatttgta
    10621 tttttattgg ctgcagccgg acttactgtt tccgcacagg aatcggctgt gaagtggtat
    10681 tccatagagg aggcggtagc tctggcacaa aagaacccca gacccatctt cgttgatact
    10741 tacaccgact ggtgcggctg gtgcaagaaa ctcgacaagg atactttttc aaaccctgtg
    10801 atagccgaga tactcaataa taaatattat gcggtaaagt ttgatgccga aggcaaggaa
    10861 cctgttgtct tccagggcag gaaattcgtc aatgacggta gccttggcaa aacccatcag
    10921 cttgcctatg ctctccttca ggggaaactc ggttacccga ccgtggtgtt ccttaccgcc
    10981 agcagcgaac ttatcactcc tgtgtcagga tataaaacac ccgaacagat tgaaccgttg
    11041 ctcaactact ttgcaggtac cggctggcag aaacagagct atgatgattt tctcaaaacc
    11101 tttaccccca aggtccggta accccgcgct gctacctgat tgcttaactg ccagttagct
    11161 gagtaagatg gaaacccgcg aagaaatagg caaatggtat gacggatttg ccgactgtca
    11221 ggaaaagacc ggtgtcaacc tgcgacacta taaaataatt gggttcctca ctggtgccgg
    11281 tctctcacgc cacagccgcg tcctggagat aggctgcggc atcggcacac tgacgggctt
    11341 gctgtcacgc tacctccgca aaggtcagat tgtggctgtt gacatcagct cccgctcagt
    11401 ggaaatagca aagaaaaggc ttaagcatgc cggcaatgtc atgctcatgg tttcagacat
    11461 gaccgacttt catgatcccg ggctattcga cttcatcgtc ctgcccgatg tcctggagca
    11521 catccctgcc agtcagcaca ggagcctctt cagcacactt gccagccata tgcatgacaa
    11581 ttcggtaata ttaatacata tacctcatcc cagagccatt gactacctgc ggaaaacctc
    11641 accggacaaa ttgcagatca ttgatcaggc catagaggct gatgccctgc ttgccgatgc
    11701 cgccgccagt gaccttgtgc tggtgaacta catttcatac tctctcttca acaaggagaa
    11761 tgactatgcg gtgattacac tgagaaagaa gtgtgcagtt acccttgccc cactgtccgc
    11821 aataatcatc agcgcaagga aaatgagcgc caggatccgg ttccttttat caaggctcta
    11881 aatcccactg atgaacagga agattctcta catatatccc tgggaagctg cctttatcga
    11941 taaagacctc gatttcctgt cacggcgcta ttctgtggtg accctggggc acgactggcg
    12001 tgacaagcga aggactcttc ccaactttct gaagcagttc atattcctct gccgcaacat
    12061 ggcgggtacc agggcaatcc ttgtaatgtt tggaggttac tggtcctttc tgcccgctct
    12121 tgccggaaag tttaccggtg tgcctgttta tatcattcca ggcggaacgg attgtgtatc
    12181 attcccttcg cttaactacg gcagcctgag gaaaccggtc atgcgcacct ttatcagatg
    12241 gtcttatcag ctctgcacca agcttttgcc agtggatgag tcactggtac tgtcagatta
    12301 cacctatctt cctgacgctg accacccgaa gcaggggttc agatatttct tcccgggtct
    12361 gcttacccct caccgggtta ttcacaatgg ctttgacccc gctttcttca ccgctgatcc
    12421 ggccggcaaa aaggagaaca gcttcattgc cgttgcccat gcacaagaga tgcagcgcgt
    12481 aagaatcaag ggaattgacc ttgttatcct tcttgcaaag cgatttacca actgcacctt
    12541 taccctggtt ggtgtctcgg attcgcttaa gcgggaactg tcacccctgc cttccaacct
    12601 gtcggtttat ccctggatgt cgaaagagaa attcatcggc cacctggctg agagccgttt
    12661 cgttctgcaa ctatctgtct ctgaaggatt tccgaatgcc ctctgtgaag cgatgttgtg
    12721 ccattgcata cctgtgggat cagcagtcgg ggctattcct tccatcatag ccgataccgg
    12781 atttgtgatt gccagcccgg aatgggaata tcttgaaaag agatttgaag agatactgca
    12841 taccggggct gaagaacagc accgccttgg tatcatggca cgcaacaggg tagcagacat
    12901 gtatcacata tcccgcaggg aaaaggcttt cctcgaaatc cttgatgaaa ttccgggctg
    12961 aggggaagac ccttttgctg tcgctacttt ccgataatga aattcgccgg cagggcaacc
    13021 aggctgccgt actggtaaat cagcctcctg gcagtgcaga gattctcccc ggcaagtgag
    13081 atgtttatct ccgccacatc cggcaccctg tagtagaccc tgtcagccgg attggcatgt
    13141 ttctgcatgg aggccggtct catcaccatg ttcagatccc tggttttgcc tgaagggcca
    13201 atctccatca gtacaggttc tcccggaccg tccggagaag tatttactcc tgatgtctca
    13261 gagaaggtaa agatggtggt cttttggccg gccatctgct ggtcaggagt gatccatatc
    13321 cggaagtgcc tggtctcagt gaagctcttg cctgcaaaca gtgccatgta ctccctctcg
    13381 agcctgttta tctcttcaag tgcggctccg tcctgcggga aaacatttgt ctcccctgtc
    13441 agaatcatat gcctcccctc acgcagctcg agcagtctgc cagcagcctc acgggcttct
    13501 tccaccagtg acattccttt tttcttctga acgaggtaag gaaccctgat gaaagcagta
    13561 tcaaccttaa ccaggcggta tgcagtgtca gtcttggtca caacatactc ataggctccc
    13621 ctgtcaggga agagcattcc tgcataatca ggatcacctt gcaggttcga atacgtggca
    13681 ctgctgtaca ggtcagggtt gatgtcaaga accagaccgc tttttctgag agccagcata
    13741 ttggtctgca tcatggcggt cccctgaatt atatagaact gtgacgggtc aagctcttcc
    13801 acggcactca gtctgatacc ggcaagactc catttctcgg tatgcccggt aataacatta
    13861 tccaggcctg tcagctcaga cgcatacctt gcatatgggc caggcacctc ggtacgtttt
    13921 tcagctataa tatcaagctc aaaaacagtc atcggcagcg cgtagaccag gcatccgtct
    13981 gtcacgctga ttttatcgcc gagctgacta accactgtat cagccggagt ttttccgctg
    14041 tacggcgagc agcctgagat catcagggca aggagagcca gaactattat actctctttt
    14101 ttcatctcaa ccattaaaga taaacattga agttactgaa gttagtctcc cggaaggccc
    14161 ttccgatatt catcaggatg tctcccgcta tcaccgcttc ctctgaatga attctcagag
    14221 ctatgtctcc tgaaaggcca tgcagatata cacctgccac tgcagcatct gagggatcat
    14281 atccctgtgc cagcagtgaa gtgattattc ccgttagtac gtcgccgctg ccgcctgtag
    14341 ccattcccgg attgccggta ctgttgaaga agagtgttcc gtcaggcagt gcaacggagg
    14401 tgtgtgcacc cttgagaacc acaacgcacc ggtaccttac ggcaaactct ctttgttcct
    14461 gcacaagcct gaagtcagtg ctcgttgtac ccgtcagcct gcggaattcc ccgggatggg
    14521 gtgtcagtac agtcagcggg ggcagcaagt caagccactc cctgttctga gcaatgatat
    14581 tcagggcgtc tgcatccagg atcaggggta cagatatgtt tttaagcagt tccctgagaa
    14641 cggaagccgt atcggggtca gttccaagtc ccgggccaac cccaatggca ttatacggtt
    14701 tcaaatcagg caatgccgtt acgtttcccg tgccgctgtc aggagagacc atggcctcgg
    14761 ggagtgctgc ctggagcgcc agtactgctg aggatggcgt atgtacagta accaggcccg
    14821 cgccggaacg cagagctgcc cctgctgaga gcgtggctgc tcctgccttg ccatagctgc
    14881 cggctatcag caaagcatga ccataggtcc ccttgtgatc aaacttgttt cttttcctga
    14941 tcatcggcct gacggtttca cgcaggacat aatgacaggg agtggccatc gatgatatgg
    15001 tatcagtatg aagcccgatg tcaattacct cccattcacc cgtgtaaata ccgtttccgg
    15061 caaacatgaa agagagcttc gggaactgga aagtgagtgt ttttgaggct ctgacaatag
    15121 agtccctgtc tgaatcctga ttatcttcgc agaaaagccc tgatggaaca tcaactgaaa
    15181 ttacaaatgc accggtttcg tttatgcgtt gtatcactcc ggcagcaact cctgacgggg
    15241 atttacgcaa acctgtccca aagatcgcat ctatgatcac ttcgtcgcgc cagattgcag
    15301 gtacctgccc gggatcggta attgtcaccg gaatgatgcc gctgcgctcc aggcgttcgg
    15361 cattcaggtt taactcactg ctgcgggccg agcctgtctc aatgacaaat accctgacta
    15421 cgtagccatc ctcatgcagc agcctggcca ggacaagccc gtcgccccca ttattgccag
    15481 gccctgcaaa cacattgatc ttcctctcag gggccacaat gtcctttacc tgctggaaaa
    15541 gagctgtggc agcacgctcc ataagtcctg ccggagtgac aggctcaagt ctgattgtgg
    15601 cggcttctat ttcccggatc tctgctgatg taaatatctt catggtgtac cgcttgctga
    15661 tagaatacaa atttaagaat atagacgatg taaaaatatc ttcattgtga tttaatccaa
    15721 tcactcttat ttaaaaagat taatatattt gtcaccgtca gaataaacta aaccaattaa
    15781 tacagaaaac tatgcttaag aatcatccaa agggcctgct tgttgccttc ttcgcgaata
    15841 tgggtgagag gtttggcttt tacaccatga tggcgattct tgtgcttttc ctgcaggcca
    15901 aatacggtct ggcagctgaa aaggcaggag acatttacag ctggttctat tttgccatat
    15961 atgcgcttgc tcttgtgggc ggtatactgg ctgactcaac ccggaaatac aagacggtga
    16021 tactgtttgg tatcataatc atgtttgccg ggtatattct tatggcagtg ccaggcatgt
    16081 cgctcacctt tgtggtcatt gccctcttca ccatagcctt cggtaacggc cttttcaagg
    16141 gcaacctcca ggctgttgtg ggccagatgt atgacgaccc gaagtattca aagctgaggg
    16201 actcggcatt catgattttt tacatgggta tcaacattgg cgccttcttt gctccatatg
    16261 cagcaaacgg tatcagaaac tggtggctta agaccaacgg atttgcccat gacggaagcc
    16321 ttccagcgct gtgtcaccag tttaccggcg gtcagatgac cgaccccgca atagtgcagg
    16381 agt
//
LOCUS       DAOPBK010000041        16266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3635_length_16266_cov_4.372587, whole genome shotgun sequence.
ACCESSION   DAOPBK010000041 DAOPBK010000000
VERSION     DAOPBK010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16266
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3635_length_16266_cov_4.372587"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLA17_03900"
     CDS             <1..919
                     /locus_tag="PLA17_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK84588.1"
                     /translation="GYIAALIVIAANLFYDIVLINNFNPVKIGAYFADTRHLLSDLLW
                     PFIYHVVKYRRGVVETNLRNAFPEKSPEERKRIAKRFYRHLTDLIVETLKAMHMSPEQ
                     IKKRFSVSDTSLADRFYSEGRDIVALCSHYNNWEWYSSMQLSSKHRVILIYKPLKNKD
                     FDRLLHKIRTRFGTWVTPMSHIIRDLVKCRNEKILTMSGFIADQTPPPDDNAFWTTFL
                     NQDTCFYRGAEKVAVKYDMPVIFINIARKKRGFYELEYSLISEHPREEEPNAIIARYA
                     AMLEEAIRVKPEYWLWSHRRWKHKREEKK"
     gene            916..1938
                     /locus_tag="PLA17_03905"
     CDS             916..1938
                     /locus_tag="PLA17_03905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK84589.1"
                     /translation="MIRTAVVILNWNGKKFLKQFLPGVVKNTLAPDTSVIVADNGSED
                     DSAKWLAENHPEVKVIRLDSNYGYAGGYNRALKEVEAEYYILLNSDVEVEPAWAEKLI
                     TFMEMHPDVGACQPRIRSYYNRDSFEYAGAAGGYIDRLGYPFCRGRIFDTIENDNGQY
                     DDVREVFWASGSCMVVRGAAFDQCDGFDESFFLHMEEIDLCWRMQNAGYTISYFPHSV
                     VWHVGGGTLGYGSPSKIYYNFRNSLIMLAKNLPAKHLRRTIFIRQVLDGLAVVMLLFR
                     EGRAAAAAVLRAHRDFRRQKTAIRTFRTLTPNPGIFTPHTTMNRYLLYEYYIRKRKTF
                     TELRWR"
     gene            1953..3095
                     /locus_tag="PLA17_03910"
     CDS             1953..3095
                     /locus_tag="PLA17_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPK84590.1"
                     /translation="MTIFITVVLTVYILTNLWLWFKGRRALSGAGINTVWYNAIFIAL
                     ASAFVIGKLLENSFTNVFIDIVNIIGGFWLAFMLYGFLAWLAADILMLLQKPFRLITP
                     DLIPKLRLWLFAGISSATILLIIIGSVNAVSPVITRYTIEVSRKPDTLHEPMHIVAVS
                     DIHLGSIIRKRSMRHLSEMITGEEPDLVLFLGDLLDGSIGPVLRGDLLSCLSIPAPRF
                     GIFAITGNHEYIGDFAKSVSYIESKGIRVLKDEIVSLENGVSIIGRLDRTAMHSPGVG
                     RKPLEQLLAESDTTAPLILLDHQPYELSALASTPVDMQLSGHTHNGQLWPLNLITDRL
                     YELSYGYRMFGKTHVIVSSGFGLWGPRMRIGTRPEILSVTLTGKSE"
     gene            complement(3087..4379)
                     /gene="murF"
                     /locus_tag="PLA17_03915"
     CDS             complement(3087..4379)
                     /gene="murF"
                     /locus_tag="PLA17_03915"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPK84591.1"
                     /translation="MEIERLYGLLRGSAGVTTDSRAVKSGELFFALKGPSHDGNRYAA
                     AALEAGALAAIVDDRSLSGERIIVVDNVLETMTALAACQRKRLQMPVIGITGTNGKTT
                     TRELITAVLSRHGRVHSTQGNFNNHIGVPLTLLSAPDDAAFLVVEMGANHMGEITALC
                     AATMPTHGIITNIGTAHIEGFGSFENVRKAKSELYQSLRENNGTAIYNDDNPILCELV
                     SGMTNTVPYSAPGGHTLSATPAGDDLMLLEVKAVIDGREHCFKTELFGSYNLDNVRAA
                     MATGLLFGIPEGEIIDAVSSYKPSNNRSQVTETGRNTVIRDAYNANPSSMEKALASFA
                     ALKAGKKMVILGDMLEMGSESAHGHAAVIRQLRSIGDVRVVLVGPLFREAAADFPATL
                     FGTSEAAAEWLRSERPEGYTILVKGSRGIMLEKVFPLL"
     gene            complement(4447..5934)
                     /locus_tag="PLA17_03920"
     CDS             complement(4447..5934)
                     /locus_tag="PLA17_03920"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPK84592.1"
                     /translation="MYKLRALPVFVVVALFLFSCKGARKDNVSPTTGWSYRDPKSSDV
                     NDLGYPIGEASEQITGPSLVLIEGGTFTMGRVDQDVMFDWNNTPRRVTVSSFYMDETE
                     ITNLDYREYLYWLRRVYSDYPEVHRRALPDTLVWRSPLALNEPYVQYYFRHPSFNNYP
                     VVGVSWLQANDYCVWRTDRVNEQILINSKWQEANVEQSGANNFNTEAYLAGQYEGIAG
                     QRVDELHPRVRQEDGILLPRYRLPTEAEWEFAAYGLRGNTFEERIYERAIYPWDGHNV
                     RNAAPKYRGEFMANFVRGRGDMMGVAGNLNDAGSITVDVRSYWPNDYGLYCMAGNVNE
                     WVMDVYRQLSSEDVDMFNPFRGNVFTDLRRDANGDVVEKDRLGRLQIDTVRPEDVAHR
                     YNYQKADYRNYLDGDVISSISSSLEWSGNADLPQTERMYSQNETDNDYVTLINDRARV
                     FKGGSWKDRAYWLNPSTRRYLDQEASRDDLGFRCAMIRVGSPSGR"
     gene            complement(6008..6973)
                     /locus_tag="PLA17_03925"
     CDS             complement(6008..6973)
                     /locus_tag="PLA17_03925"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPK84593.1"
                     /translation="MKLRNRTLAAALLICTLTLSGQDTWYGSLSGMQVMYNPAFAGVE
                     GVSAIKISAFSFLPGNGFGLRSVYASYDGYFRSIRGGAGAWVSDDIPGDVMHDLRGGA
                     FYSYHFRAGRRLYVTAGLTAAMISRNIRSGSVILPGDIDPFRGITGGGTEYIPPAPIT
                     RFDLGTGFTVSSADWFAGASIIHLTRPSLSDDALEHNRVNRLLTLTGGLSLTPGRKDF
                     SLLPSAALLIQGDEARIYLGSEASWKGLAGALSLWHVTNGFTALGASAGWEVATVKIM
                     LSYSYIISGGGASFGGTSMVKAGVMFSFGNVEKSRGAHIIKLPLL"
     gene            7079..10480
                     /gene="porU"
                     /locus_tag="PLA17_03930"
     CDS             7079..10480
                     /gene="porU"
                     /locus_tag="PLA17_03930"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPK84594.1"
                     /translation="MMKSVVTGLMVMAFVLNAASQDYAASSVLGSGQWVKIAVTEEGI
                     YRLDYSHLKEMGFTDPSSVVLYGNNAGQLSFMNDNSAPDDLRKVAFRAEKGADGVFNE
                     GDYLLFYAEGTHRWVADPGTRSRRFVRHHYSDTAWYFITSQPGEALPVLTETPPATLP
                     TFISSSTDLYMRHEQEEINLIRSGREWYQQVVPGTQNSIGPGFTDLAASGKIHYTIRV
                     AARSGTETSFILREDGETIKTITVPAVTMTDFNGVYAATMTVSDSVVPSSGSPAFTLA
                     FSSGGNMAASGYIDYADFKARASLVWRNRNLFISDSRSVGTSTVTRFTVEGSSSLSIW
                     DITNPYAPLAIETTPSSGNTLFMAATDSLRRFIAFSMAQVKQPLRSVRVPAQNLHAMM
                     PAGMIIVTHPLFMTCAGQLADLHLADDGLTSLIVTPEQIYNEFSGGVPDAVAIRNFVR
                     MIWKRGMQSGPQMRYLLLFGDGSYENKTPPPGNSSFIPTWQSVNSTTGVLSFTSDDFY
                     GLLDDGEGEADGFLDIGIGRLPAYDTASAGIMIRKISSYLQGSNQGSWRNILCLVADD
                     EDSNLHMFDAEGLAEAATTAAPPLMVEKIYLDAYRQIGSVSGDSYPDASRAIDNRMAA
                     GCLIMNYVGHGNESGLAHERVIRTDNINSWKNSKMLPLFITATCEFSRFDDVEINRAS
                     SSITARNSAGEMVLLNPDGGGIALMSTTRVVYSAPNYTLNRAIYDYAFRTDADGRSMR
                     FGDIIRQAKVSSGAGMNKRSFLLLGDPALRLAWPSTGKVVTDSINGVHVSMPFDTLRA
                     LSLMTVSGHVEDAAGNLMTGFNGTVEPVVSDKPGYVSTLANDGGSPMTFPVDGNVIFR
                     GKTTVTDGRFSFSFIVPLDINYSYGSGSVKYYAYNGVDDLNGSFPGITVGGFSDAAFD
                     DTEGPQIRLFMNDTLFNNGGVTDTSPILLALLSDRSGINTSGAGIGHDIIAWLDDDIS
                     GAVVLNSLFRADVGKHSSGSIAYPLLVTDQGRHTVSLRAWDNLNNPTVATLEFTVETS
                     GNFRLTDLLTFPNPVTDGTKFTAGHNRPGTEISLTITILSSDGRVVKVIKSDFYSDGY
                     ALPDIPWDGCDEKGGRVARGLYLWRAEAITANGEKTSATGRFIIL"
     gene            10640..>11051
                     /locus_tag="PLA17_03935"
     CDS             10640..>11051
                     /locus_tag="PLA17_03935"
                     /inference="COORDINATES: protein motif:HMM:NF040321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPK84595.1"
                     /translation="MVVPFLTIAPDSRAGALGNAGVATSADLYSMHWNPAKYAFIEGK
                     GGFAMSYVPWLRALVNDINMSNISGFYRIDNRQVVAASLMYSALGRIQFTNDQGGVLM
                     DMNPNEFSVDAAYSRLFSEHWSGGVALRFIYSNLT"
     assembly_gap    11052..11151
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11152..11853
                     /gene="porV"
                     /locus_tag="PLA17_03940"
     CDS             <11152..11853
                     /gene="porV"
                     /locus_tag="PLA17_03940"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HPK84596.1"
                     /translation="SEHWSGGVALRFIYSNLTGGQTVGSTETDVGTAFAADASVFYTT
                     PLKLGAKTGNYAAGLNFSNIGSKMSYTADNDPDFIPMNMRLGTTFSIDLDKYNTIEFS
                     LDINKLLVPTPPFYDEDENITDGMDPNVSVPVAIFQSFYDAPGGFKEELQEFTQSFGI
                     EYWYNQQFALRAGYHNEAMYKGNRKFFTAGAGFKLSLINIDFSYLISLTQNSPLAGTV
                     RFSLGFEFDSLRSQK"
     gene            11864..12364
                     /gene="ispF"
                     /locus_tag="PLA17_03945"
     CDS             11864..12364
                     /gene="ispF"
                     /locus_tag="PLA17_03945"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009231428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPK84597.1"
                     /translation="MTLHHRIGQGIDFHRLEEGRELWLGGVRIDSTKGCVAHSDGDVL
                     IHAICDALLGAAGLNDIGYHFPDTSEEFRAIDSKILLKRVVELITGAGYRIVNIDSTV
                     CLEQPKLAPYITAMRTTMAAIVNISPVAVSVKATTTEKMGFTGRGEGVMALAVCLISD
                     LIPDNI"
     gene            complement(12489..14498)
                     /locus_tag="PLA17_03950"
     CDS             complement(12489..14498)
                     /locus_tag="PLA17_03950"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPK84598.1"
                     /translation="MNDFKSQVSEGIPSHLPAPKAWDPAVNHAPRRRDILTAEEKKLA
                     LRNALRYFPPSLHRVLAPEFAAELSERGRIYMYRYRPDYEMKARPIGDYPHRSAQAAA
                     IMLMITNNLDPAVAQHPHELITYGGNGAVFQNWAQYRLTMKYLSEMTDEQTLVMYSGH
                     PLGLFPSHREAPRVVVTNGMVIPNYSSQDNWERFNALGVSQYGQMTAGSYMYIGPQGI
                     VHGTTITLLNAKRRVKPVDPEGRRSRLFISSGLGGMSGAQPKAGNIAGIVSVIAEVNP
                     KAIETRYKQGWVDEVYTDLSELVVRVRKACSDGEVVSLAFHGNIVDLWERFSDEGVWA
                     DLGSDQTSLHNPWAGGYYPAGISFDEANRIMAEDPEKFRALVQGSLRRQVEAINRHTA
                     HGTYFFDYGNAFLLEASRAGADIGNGRGGFRYPSYVEDIMGPMCFDYGFGPFRWVCCS
                     GDPADLEKSDAMAAGTLESLMKESPEDIRQQMSDNIHWIREAGKNRLVVGSQARILYA
                     DAAGRIMIASAFNDAIASGVISGPIVIGRDHHDVSGTDSPFRETSNIYDGSRFTADMA
                     VQNVIGDAFRGATWVSLHNGGGVGWGEVINGGFGMLLDGTPETSRRIRSMLFWDVNNG
                     IARRSWARNSGAVAAITREMERTPGLVVTMPQEADENLIDEVFGQ"
     gene            complement(14617..15384)
                     /locus_tag="PLA17_03955"
     CDS             complement(14617..15384)
                     /locus_tag="PLA17_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPK84599.1"
                     /translation="MTKETKLAETELSIEEKLRALYSLQLVDSEIDKIRILRGELPLE
                     VQDLEDEVAGLETRITNLKGEVSELEKSVIGKTNEITASQALIKKYEEQQNNVRNNRE
                     YDSLSKEIEFQTLEIELCNKKIREFTAQTADKKEMMTEAQTMLEERKRDLESKKSELE
                     EITHDTQKEEEQLTVKSVELQTRIEERLLMAYKRIRSNARNGLAVVPVKRDACGGCFN
                     QIPPQRQLDIRSRKKIIVCEYCGRILVDDEIVGEAKQ"
     gene            complement(15368..>16266)
                     /locus_tag="PLA17_03960"
     CDS             complement(15368..>16266)
                     /locus_tag="PLA17_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPK84600.1"
                     /translation="KRLAYGSNSETSVASAIRKGIAVYSAHTTLDNAGNGVSRILAEK
                     IGLESIRVLEPLRDRLLKLVTFVPESHAAAVRDALFAAGAGNIGNYSSCSFNARGEGT
                     YRAGEGADPFAGSIGELHTEPEVRIEVVLPSHLKGACLKAMLAAHPYEEVAWDLIALE
                     NEYTGAGAGAIGALAAPLTGTALLERLRRLTGLPVIRYSNDPARTVSTVAVCGGSGSH
                     LISSAARAGADAFITGDIKYHAYAEAPRDMLVVDIGHYESERFSLEILYNLIQKKFPN
                     FALRFSGIKTNPINYYGYDKRD"
BASE COUNT         3875 a         4368 c         4022 g         3901 t
ORIGIN      
        1 aggttatatc gccgcgctta tcgttatcgc ggcaaatctt ttttatgata tcgttttaat
       61 caataatttc aatcccgtca aaatcggcgc atattttgcg gatacgcggc atctgctgtc
      121 agacctgctg tggccgttca tctatcatgt cgtaaagtac cgccgcgggg tagtagagac
      181 aaacctcaga aatgcatttc cggaaaagag cccggaagaa cgtaaaagga tagcaaagag
      241 attctacagg catctcactg acctgatagt ggagaccctg aaggctatgc acatgtcacc
      301 ggagcagatc aaaaagcgtt tttccgtttc cgacacctcc cttgcagaca ggttctacag
      361 cgaaggacgt gatattgtcg ccctttgcag ccactacaac aactgggagt ggtactcctc
      421 aatgcagctc tcatcaaaac acagggttat acttatttat aaaccactga aaaacaagga
      481 ttttgaccgg cttctacata agataagaac aaggttcggg acatgggtca cacccatgag
      541 ccatatcata agggatctgg taaaatgcag gaatgaaaag attctcacca tgtcaggatt
      601 cattgcggac cagacacctc cccccgatga caatgcattc tggacaacat tccttaatca
      661 ggatacatgc ttctaccggg gggcggaaaa agttgcggtt aaatatgata tgccggtaat
      721 atttattaat atagccagga agaaaagagg cttttacgaa cttgaataca gtctgatatc
      781 agagcacccc cgcgaagagg aacccaatgc cataattgcc cgttatgccg ccatgcttga
      841 agaggcaatc agggttaagc ctgaatactg gctatggtca caccggcggt ggaaacataa
      901 aagagaggag aaaaaatgat cagaacagcc gtagtcatcc tcaactggaa cggaaaaaaa
      961 ttccttaaac agtttcttcc gggtgtagtt aagaatacgc ttgcacctga cacatcggtc
     1021 attgtggctg ataatggatc cgaagatgat tcagcaaagt ggctggcgga aaaccacccg
     1081 gaggtcaagg ttatcaggct tgacagcaac tatggatatg cgggaggtta caacagggca
     1141 ctgaaggagg ttgaagccga atactacata cttctgaatt ccgacgtgga ggttgagccg
     1201 gcgtgggcag agaagctgat cacctttatg gagatgcacc ctgacgtggg tgcctgccag
     1261 cccaggatca ggtcatacta caaccgtgac tcgtttgagt atgcaggcgc tgcaggaggg
     1321 tatattgacc ggctgggcta ccctttctgc cgtggacgta tctttgacac aatagagaat
     1381 gacaacggcc agtatgacga tgtccgcgag gtcttctggg catcaggctc atgtatggtc
     1441 gtcaggggtg ccgcattcga ccagtgtgac ggctttgacg agtcattttt cctccacatg
     1501 gaagagattg acctctgctg gaggatgcag aacgccggat ataccatcag ctactttcct
     1561 cattccgtgg tctggcacgt gggaggaggc accctcggtt acggatcacc atccaagatc
     1621 tattacaact tccgcaacag ccttatcatg ctggccaaga acctcccggc aaagcatctc
     1681 cgcagaacca tcttcatccg ccaggtgctt gacggcctgg cagttgtcat gctgctcttc
     1741 agggaaggcc gcgcagcagc agcagcggtt ttaagggcac acagggattt caggaggcag
     1801 aagacagcta tcagaacctt cagaaccctg acacctaacc cgggaatctt cactccgcat
     1861 accactatga acagatacct cctttatgag tattatatac gcaaaaggaa aacatttaca
     1921 gaactcaggt ggcgctgaac aggaagcagc agatgacaat attcattacg gtagtactaa
     1981 ccgtctatat ccttaccaac ctgtggctct ggttcaaagg ccggagagct ctctcagggg
     2041 ccggaataaa caccgtatgg tacaatgcaa tcttcattgc cctggcatca gccttcgtga
     2101 tcggcaaact ccttgaaaac tctttcacaa atgttttcat agacatagta aacattatcg
     2161 gcggcttctg gctggcgttc atgctctacg gattccttgc atggctggct gcggatatcc
     2221 tgatgctgtt gcagaagcct ttccgcctga tcactccgga tctcatcccg aaactcaggc
     2281 tgtggctgtt tgcaggaatc agctcagcaa cgatcctgct gatcattatc ggatctgtta
     2341 atgcggtaag cccggttatt accagataca ccatcgaagt cagcaggaag cctgacaccc
     2401 ttcatgaacc gatgcacatc gttgccgtct ctgacataca ccttggcagc attatccgga
     2461 agagaagcat gcgccatcta tcggaaatga taaccggtga agagcctgac ctcgtcctgt
     2521 tccttggcga tctgctcgac ggaagcattg gtccggtgct gcggggtgac ctgctctcct
     2581 gtctcagcat ccccgcacca cggttcggga tctttgccat tacagggaac catgagtata
     2641 taggtgactt tgcaaagtca gtatcatata ttgaaagcaa gggaataagg gtgctgaaag
     2701 atgagattgt cagccttgaa aacggtgtca gtataattgg ccggcttgac agaacagcca
     2761 tgcattcacc gggcgtcggc cgcaagccgc ttgagcaact gctggctgag agtgacacca
     2821 ctgctccatt gattctgctt gatcaccagc cttatgagct gtcagccctg gcctcaacac
     2881 cggttgacat gcaactctca gggcataccc acaacgggca gctatggccc ctcaacctta
     2941 tcacagacag gttgtatgaa ctgagttacg gatacaggat gttcggaaag acacacgtga
     3001 tagtctcttc cggatttggt ttatggggtc cccggatgcg gataggaacc aggccggaga
     3061 tactttctgt taccctgacg ggaaaatcag agtaagggga aaactttttc aagcattata
     3121 cccctcgatc ctttgacaag gatggtatag ccttcgggtc tctctgacct cagccattcg
     3181 gcggcagcct ctgatgttcc gaagagggtg gccgggaaat ctgctgccgc ttcccggaaa
     3241 aggggaccca caagcaccac cctgacgtca ccgatactcc tcagttgcct gataacggca
     3301 gcatggccgt gagcgctctc actgcccatc tccagcatat caccaaggat aaccattttc
     3361 tttcccgcct tcagcgcagc aaatgaagcc agcgccttct ccatgctcga gggattggca
     3421 ttgtatgcat cacggataac ggtattcctg cctgtctccg tcacctgtga ccggttattc
     3481 gagggcttgt atgatgaaac tgcatcaatt atctctcctt ccggaatgcc gaaaagcagt
     3541 ccggttgcca ttgcagccct tacattgtca aggttgtatg atccgaagag ttctgtttta
     3601 aaacagtgct ccctgccgtc aatcaccgct tttacctcca gcagcatcag gtcatcaccg
     3661 gcaggagtgg cgctgagagt atgtcctccc ggtgccgagt agggcacggt attggtcatc
     3721 cctgatacca gttcacacag gatgggattg tcatcgttgt atattgcagt gccgttattt
     3781 tcccgtaatg actggtatag ttcggacttc gcttttcgca cattttcgaa cgagccgaaa
     3841 ccctctatat gtgccgtgcc gatattggtg atgatgccat gtgtaggcat tgttgcagca
     3901 cagagggcgg ttatctctcc catgtggttg gcacccatct ccaccaccag gaatgctgca
     3961 tcatcaggtg cgctgagcag ggtcagcgga acacctatgt gattgttgaa gttgccctgg
     4021 gtgctgtgta cccttccgtg ccgcgacaga acggcagtga tcagctccct ggtggttgtc
     4081 ttgccgttgg tgccggttat cccgatgacg ggcatctgca gcctcttccg ctgacaggcg
     4141 gccagggctg tcatggtctc aaggacattg tcaacaacaa ttattctttc tccgctgagc
     4201 gaccggtcat ccactatggc ggccagcgct ccggcctcca gggcggctgc ggcatacctg
     4261 ttcccgtcat gggaaggacc cttgagtgca aagaaaagct caccactctt tactgccctc
     4321 gaatccgtcg ttacaccggc agaaccccgt aacagaccgt acaacctttc aatctccatc
     4381 ctgttttaaa aagaagcctc attactttgg ataatgaggc tttattctta catgtttata
     4441 tatacattat cgacccgagg gacttcctac cctgatcatt gcacacctga aaccaaggtc
     4501 atcacgtgat gcttcctgat caagatagcg gcgggttgag gggttgagcc agtaagccct
     4561 gtctttccat gatccgccct tgaataccct tgccctgtca ttgatgaggg taacatagtc
     4621 attatcagtc tcattctgtg aatacatcct ctctgtctgg ggcaggtcgg catttcctga
     4681 ccattcaagg cttgaggaga tgctggagat gacgtcaccg tcgagatagt tcctgtagtc
     4741 tgccttctga tagttgtacc tgtgggctac atcttccggc ctgacagtgt caatctgcag
     4801 tcttcccagc ctgtccttct caaccacatc gccgttggcg tcacgcctca gatcggtgaa
     4861 cacgttaccc ctgaacgggt tgaacatatc cacatcctct gaggagagct gacggtaaac
     4921 gtccatcacc cactcgttaa cgtttcctgc catgcagtag aggccatagt cattgggcca
     4981 gtatgatctt acgtcaaccg tgatacttcc cgcgtcgttc aggtttccgg caacacccat
     5041 catgtcaccg cgtccgcgaa cgaagttggc catgaattca cccctgtact tgggggctgc
     5101 gttacggacg ttgtgaccgt cccacgggta gatagccctt tcatatatcc tctcctcgaa
     5161 ggtgttgccc ctgaggccgt aggctgcaaa ttcccactcg gcctccgtcg gcaggcggta
     5221 gcgcggaagc aggatgccat cctcctggcg gacacgcgga tgaagctcat caactctctg
     5281 cccggcgata ccttcatact gacctgccag ataggcttca gtattgaagt tattggctcc
     5341 tgactgttcc acattggctt cctgccactt gctgttaata agaatctgct cgttgaccct
     5401 gtcagtacgc cagacacaat agtcattggc ctgcagccag ctgacaccca caaccggata
     5461 attattgaac gaggggtgac ggaagtagta ctgaacgtat ggttcattca gcgcgagcgg
     5521 acttctccag accagagtgt cagggagagc gcggcggtga acctccggat aatcgctgta
     5581 caccctcctc agccagtaca gatactcacg gtagtcgagg ttggtgatct ctgtctcatc
     5641 catataaaag gaggaaacgg ttaccctgcg cggcgtgttg ttccagtcaa acattacatc
     5701 ctgatcaacc cgccccatgg taaaggtacc accttcaatc agcaccaggc tcggaccggt
     5761 gatctgctcc gaagcctctc ctatcgggta acccaggtca ttgacatcgc tcgatttggg
     5821 atccctgtag ctccagccag tagttggaga cacattatcc tttctggctc ccttgcagga
     5881 aaaaaggaag agagcaacaa ctacaaaaac cggtaaagct ctgagtttat acatagcaat
     5941 ataaggttac ttgattactt tttagtttaa aaactgtatc catttgcata actctgcacc
     6001 aaaataacta caaaaggggc aactttatta tatgtgctcc cctacttttt tcaacatttc
     6061 cgaaactaaa catcaccccg gcttttacca tggaggtgcc cccgaatgaa gcgccaccac
     6121 ctgagataat gtaactatat gataacatga tctttactgt tgccacctcc catccggcgg
     6181 aagctcccag agccgtaaat ccgttagtga catgccatag tgaaagagcg ccggcaagac
     6241 ccttccacga ggcttcgctc cctaaataaa ttcttgcctc atcaccctgt ataagtagcg
     6301 cagctgatgg caacagggaa aaatcctttc tgccgggtgt gagcgagagt ccccctgtca
     6361 gtgtaagcaa ccggttcacc ctgttgtgct caagggcatc atcgctgagc gagggcctgg
     6421 tcagatgtat gatcgacgca ccggcgaacc agtctgctga tgatacggta aagcctgttc
     6481 ccaggtcgaa acgtgtaatg ggggcaggag gaatatattc agtgcctccg ccggttatgc
     6541 ccctgaaagg gtcgatgtca cccggaagga taaccgatcc gcttcgaata ttccggctga
     6601 tcatcgcggc tgtcagtcct gcggtaacat agagcctgcg accggcacgg aagtggtatg
     6661 aatagaatgc tccgcccctg agatcatgca tgacatcccc cggaatatca tcagagaccc
     6721 aggcacctgc cccaccccgg atggacctga aatacccgtc atatgatgcg tacacagacc
     6781 tcagcccgaa gccgtttccc ggaagaaagg aaaatgcaga aatctttatt gcagagacgc
     6841 cctcaacacc tgcaaatgcc ggattataca ttacttgcat accagacagt gacccgtacc
     6901 atgtatcctg gccactgagg gtcaacgtgc agatgagcag cgccgcagca agagtcctgt
     6961 ttctgagctt catggttgtg tttcagtatt atcaacgctg ccgggagttc attattttgc
     7021 ctgcagctgc atcaatacgg gagcaaagat agatgacttg actatctttg ccatgattat
     7081 gatgaaaagt gttgtcaccg gactcatggt tatggccttt gtgctgaatg ctgcttcgca
     7141 ggattatgcg gcttcatcag tgcttggcag cgggcagtgg gtaaagatag ccgtgaccga
     7201 agagggcatt taccgtctcg actattccca tctgaaggag atgggattca ctgacccctc
     7261 ttcagtggtc ctttacggca acaatgccgg ccagctctca ttcatgaacg ataactccgc
     7321 ccctgatgac ctgagaaagg ttgccttcag ggcagaaaag ggagccgacg gagttttcaa
     7381 cgaaggtgat tatctcctct tttatgctga aggcactcac cgttgggttg ctgaccccgg
     7441 cacgcgcagc cgccggttcg taagacatca ttactcagat acggcatggt atttcatcac
     7501 ctcgcagccg ggagaagccc ttcctgttct gacagagact ccgccggcaa cattacctac
     7561 attcatatcc tcatcaactg acctgtacat gagacacgaa caggaggaaa tcaacctcat
     7621 aaggtcagga agggagtggt accagcaggt ggttcccggt actcagaaca gcatcggacc
     7681 gggatttaca gacctcgctg ccagtggaaa aatacactac accataaggg ttgccgcgag
     7741 gtctggcaca gagacctcct tcatcctcag agaggatggt gaaacgatca aaaccataac
     7801 cgtaccggca gtgaccatga ccgatttcaa cggtgtttat gccgccacga tgactgtttc
     7861 agattcagtc gtcccctcct ccggatcacc ggcattcact ctcgcattct catcaggcgg
     7921 caatatggcc gctagcggct acattgacta tgccgatttc aaagcgaggg cttcactggt
     7981 ctggcgcaac aggaacctgt tcatcagcga cagcaggtct gttggcacgt cgacagtcac
     8041 ccggttcacc gtggagggct cctcttcgct gagcatctgg gacatcacca atccctatgc
     8101 cccgctggcg atagagacaa ctccctcctc cgggaacaca ctctttatgg cggcaactga
     8161 cagcctcagg agattcattg ccttcagcat ggcacaggtg aaacagcctt tgagaagcgt
     8221 ccgggtgcca gcccagaacc tgcacgccat gatgcctgcc ggcatgatca ttgtcacaca
     8281 cccgctcttc atgacctgtg ccggacaact tgcagacctg catcttgctg atgacggcct
     8341 gacatcattg atagtgaccc cggaacaaat ctacaatgag ttttcgggag gggtgcccga
     8401 tgcggttgca ataagaaatt tcgtcaggat gatctggaag agggggatgc agtcaggtcc
     8461 gcaaatgagg tacctgttgc tctttggtga cggctcgtat gagaataaaa cccctccgcc
     8521 gggaaacagc tcattcatcc ccacatggca gtctgtcaac tcaacaaccg gagtactctc
     8581 cttcacttca gacgattttt acggactgct tgatgatggc gagggtgaag ctgacggatt
     8641 ccttgacata ggcatcggcc gtctgccggc gtatgacacc gcatcggccg gcattatgat
     8701 aagaaaaatt tcatcctacc ttcagggatc aaaccagggt tcatggcgga atatcctgtg
     8761 ccttgttgca gacgatgaag attccaatct gcatatgttt gatgctgaag gccttgccga
     8821 agcagccacc accgcggcac cgcccctgat ggtggagaaa atatatctcg atgcttaccg
     8881 gcagattggt tctgtctccg gcgattccta tcctgacgct tcaagggcta ttgacaacag
     8941 gatggcggcc ggatgcctga taatgaacta cgtgggtcat ggcaacgaat caggcctcgc
     9001 ccacgaaaga gttattcgta cagacaatat caactcgtgg aagaacagca agatgcttcc
     9061 gctgttcatc accgcaacat gtgagttctc aaggtttgat gatgtggaaa tcaacagggc
     9121 ttcctcctcc ataaccgcaa gaaactcagc cggcgagatg gtgcttctca atcctgacgg
     9181 cggagggatt gccctgatga gcacaaccag ggtcgtctac tcagctccga actatacact
     9241 gaacagggcc atctatgatt atgctttcag aacagatgct gatggccgat cgatgaggtt
     9301 tggcgacatt atcaggcagg ccaaggtcag ctccggagcg gggatgaaca aacgaagctt
     9361 cctgctgctg ggtgaccctg ctctcaggct ggcatggcct tcaacgggga aagtggttac
     9421 tgacagtatc aacggggtcc atgtctcaat gccttttgac accctgaggg cactgtcact
     9481 gatgactgtc agcggtcacg tggaagatgc cgcaggaaac ctgatgaccg gattcaacgg
     9541 cacagtggaa cctgtggtat ctgacaagcc cggatatgtc agtaccctgg ccaatgacgg
     9601 tggttcccca atgaccttcc cggtggatgg taacgtcatc ttccggggta aaacaactgt
     9661 caccgacggc cgtttcagct tctcattcat cgtacccctt gacatcaact acagctatgg
     9721 cagcgggtca gtgaaatact atgcatataa cggcgtcgac gatctcaacg gaagcttccc
     9781 gggaattacg gtaggaggct tctctgacgc ggcattcgat gatactgaag gaccacagat
     9841 caggctcttc atgaatgaca ccctgttcaa taatggtggg gtgactgaca cctcgccaat
     9901 actgctggca ctgctcagcg acagatcagg cattaatacc tcaggggcgg gtataggaca
     9961 tgacataata gcatggcttg atgatgacat ctccggggcg gtggtgctta acagcctctt
    10021 cagggctgac gtgggcaaac acagttcagg ctccattgcc tatccacttc ttgtgaccga
    10081 ccaggggaga cacacggtct ccctgagggc ctgggataac cttaacaacc cgacagtggc
    10141 cacgcttgag tttacagttg aaacaagcgg caacttcagg ctcacggacc tgctcacatt
    10201 cccaaatccg gtgactgacg gcacaaaatt tactgccggg cacaacagac cgggtacaga
    10261 gatcagcctg accataacaa ttctcagttc tgacggacgt gtggttaagg taataaagag
    10321 tgatttttac agcgacggtt atgcccttcc cgacatccca tgggacggct gcgacgagaa
    10381 gggcggaagg gtggcaagag gactctatct gtggcgtgca gaagcaatta ccgcaaatgg
    10441 cgagaaaaca tcagccacag gccgtttcat cattttgtaa aacattacaa taagatgccc
    10501 ttatttttgt tttatatttg cacagatttt ttggatatat ctagatatga aaaataatgc
    10561 gctgattgct ggcataatat tgctattata tctgtttacc gttccggtta ctgcacagga
    10621 ggataataga acaattcagg tggttgttcc ctttctgacc atcgcacctg attctcgtgc
    10681 cggagcactg ggaaatgccg gagttgcaac ctctgcagat ctgtacagca tgcactggaa
    10741 ccctgccaaa tatgccttta ttgagggtaa gggtggcttt gccatgtcat acgtaccatg
    10801 gctgagagcc ctggttaacg acataaacat gtcaaatatt tcaggattct accggattga
    10861 caacaggcag gtagttgctg ccagtcttat gtattcagca cttggcagga ttcagttcac
    10921 aaatgatcag ggaggcgtat taatggatat gaatccaaat gaattttcag ttgatgctgc
    10981 ctattcgcgc cttttctcag agcattggtc cggtggcgtt gcactcagat ttatctattc
    11041 aaacctgacg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcagagcat
    11161 tggtccggtg gcgttgcact cagatttatc tattcaaacc tgacgggcgg acaaactgtt
    11221 ggatcaactg aaacagatgt aggtactgct ttcgcagcag atgcctctgt cttttatact
    11281 acaccgctga agctgggtgc caaaaccggt aactacgcag ccggcctgaa cttctccaac
    11341 ataggttcca aaatgagtta cacagccgat aacgatccgg acttcatacc aatgaacatg
    11401 cgtcttggaa ccacgttcag cattgacctt gacaagtaca atacaatcga attctccctt
    11461 gacatcaata aactgctcgt gccaacccct ccattctatg acgaggatga aaatattact
    11521 gacggcatgg atccgaacgt atcagttccc gttgcaatct tccagtcatt ctatgatgct
    11581 cccggaggat tcaaggaaga gctccaggag tttacccaat ccttcggtat cgaatactgg
    11641 tataatcaac agtttgcact cagggcaggc tatcataacg aagccatgta caaaggcaac
    11701 agaaagtttt tcactgccgg tgccggtttt aagctcagcc tcataaatat cgacttctca
    11761 taccttattt ctcttacaca aaacagcccg cttgcaggta ctgtgagatt ttcactgggg
    11821 tttgaattcg attccctgcg cagccagaag taagctaacg gttatgacac tccatcacag
    11881 gataggacag ggcatcgact ttcaccgcct tgaggagggt cgtgagctct ggctgggtgg
    11941 cgtacgcatc gactctacaa agggatgtgt ggctcactct gacggcgatg tccttatcca
    12001 tgccatctgt gatgcacttc tgggtgcggc aggacttaat gacatcggct atcacttccc
    12061 cgatacatcg gaggagttca gggcgattga cagtaagata ctgctgaaac gggtggttga
    12121 gctgatcacc ggcgcaggtt acaggatagt aaacattgac agtactgtct gcctcgagca
    12181 gccgaagctg gcgccatata tcaccgccat gcgcacaacc atggccgcaa ttgtgaatat
    12241 ctcacccgtt gctgtttcgg ttaaggctac caccactgaa aagatgggtt tcaccggccg
    12301 cggcgaaggg gtcatggccc tggccgtctg cctcatctcc gacctgatcc cggataatat
    12361 ctgatagtct gaaagcctga gaatctgaat gttgtgccgc aaatcactgc tgcacgctgc
    12421 ctgattgcct ggtgcctgat tatctgttgc ctgattgcca gttgcccgat tgcctgttac
    12481 ctgattgcct actgcccgaa aacctcatct ataaggtttt catcagcctc ctgcggcatg
    12541 gtgacaacca gcccgggggt gcgttccatt tcccttgtta tggctgccac ggcgccgctg
    12601 ttacgtgccc agctgcgacg ggcaataccg ttgttgacat cccagaacaa catggaccgg
    12661 atgcggcgcg atgtctccgg agtgccgtcg agcagcatcc cgaaaccgcc gttgatcacc
    12721 tcgccccagc ccacaccgcc gccgttgtga agggagaccc atgtggcccc gcggaaagcg
    12781 tcgccaatga cattctgtac tgccatgtca gccgtgaagc gtgaaccgtc atagatgttc
    12841 gatgtctcac ggaacggtga gtctgtgcct gacacatcgt gatgatcacg gccaattact
    12901 atcgggccgc tgatgacccc cgaagctatt gcatcgttga aggccgaggc aatcattatc
    12961 cttccggcgg cgtcggcata aaggatacgc gcctgggaac caaccaccag gcgattcttt
    13021 cccgcctcgc ggatccagtg aatattgtca gacatctgct gacggatatc ctcaggtgac
    13081 tctttcatga gactctcaag agtgccagca gccatggcat cactcttctc caggtcggca
    13141 gggtcaccgg aacagcagac ccagcggaac ggaccgaagc cgtagtcaaa gcacatggga
    13201 cccatgatat cctcaacgta agacggataa cggaatcctc ccctgccgtt acctatatcg
    13261 gcgccggcac gcgaagcttc cagaaggaag gcattgccat aatcaaagaa ataggttccg
    13321 tgtgccgtat gtctgttaat ggcctccacc tgccggcgca gcgaaccctg caccagtgcg
    13381 cggaacttct ccgggtcttc agccataatc ctgttagcct catcaaagct gatccctgca
    13441 gggtagtaac cacctgccca cggattatgc agcgaggtct gatccgatcc caggtcagcc
    13501 cacactcctt catcagaaaa cctctcccac aggtcaacaa tatttccatg aaaagccagt
    13561 gacaccacct caccgtcact gcaggcctta cggacccgca ctaccagctc tgacagatca
    13621 gtatacacct catcaaccca gccctgcttg tagcgcgttt caatagcctt cgggttgacc
    13681 tcggcaatga ctgacacaat cccggcaata tttcccgcct tgggctgagc ccctgacatg
    13741 ccccccagcc ctgacgagat gaagagcctg ctcctgcgtc cttcagggtc aaccggctta
    13801 acccgtcgct ttgcattgag gagggttatg gtggtgccgt gaacgatccc ctgcggaccg
    13861 atatacatat atgatccggc agtcatctgt ccgtactgtg acacaccaag ggcattgaac
    13921 cgctcccagt tatcctgcga cgaatagttt gggatcacca tcccgttggt gacaaccacc
    13981 cgcggcgcct ccctgtgtga cgggaacagg ccgagggggt gccccgagta catcaccagc
    14041 gtctgctcat cagtcatctc agagagatac ttcatggtga gcctgtactg ggcccagttc
    14101 tggaatacag ctccgtttcc gccataggtg ataagctcgt ggggatgctg ggcaacagca
    14161 ggatcgaggt tgttggtgat catcagcatt attgcggcag cctgcgccga gcggtgcgga
    14221 taatcgccta tcggacgggc tttcatctcg tagtcaggcc tgtaacgata catgtatatg
    14281 cgtccccgtt cgctgagctc ggctgcaaat tccggagcca gtaccctgtg aagcgacggg
    14341 ggaaaatacc tgagggcatt gcggagggca agttttttct cctcagcggt gagaatgtca
    14401 cgcctgcgcg gggcatgatt caccgcgggg tcccaggctt tcggtgcagg aaggtgtgat
    14461 ggtatgcctt cactaacctg tgatttgaaa tcattcatga tattggataa agttcagtat
    14521 tgtaaagata ataaaaagag agagcgacac cttgcgtggt gcctctctca atatcattca
    14581 acatggcaat accctgttgc tgtatggtac tgccagctat tgcttcgctt ctccgacgat
    14641 ctcatcatca acaagtatgc ggccgcaata ctcgcagaca atgatctttt tcctcgacct
    14701 gatgtcaagc tggcgctgtg gtggtatctg gttgaagcat ccgccgcatg catccctctt
    14761 caccggcacc accgccagac cgttgcgggc attgctgcgt atgcgcttgt atgccatcag
    14821 caacctctcc tctattctcg tctgaagttc aactgatttt acagtaagct gctcctcctc
    14881 cttctgggtg tcatgggtta tctcctccag ctctgacttc ttgctctcca ggtcacgttt
    14941 ccgctcttca agcattgtct gtgcctcagt catcatctcc ttcttatctg cagtctgggc
    15001 ggtgaactca cgaatcttct tgttgcacag ttctatctcc agtgtctgga actcaatctc
    15061 ctttgagagg gagtcatact cacggttgtt gcgtacgttg ttctgctgct cctcgtattt
    15121 ctttatcagt gcctgagagg cagttatctc gttggtcttg ccaatgacag atttttccag
    15181 ttcggaaact tcgcctttaa gattggttat tctggtctcg agccctgcca cctcatcttc
    15241 aaggtcctgt acctcaagag gcagctcgcc acgaaggatg cggattttat ctatctctga
    15301 atcaacaagc tgtaatgagt agagagctct cagtttctct tctatggaaa gttctgtttc
    15361 agccagttta gtctcttttg tcatacccgt agtaattaat gggattggtt tttatcccgg
    15421 aaaaccggag tgcaaagtta ggaaattttt tttgaatcag gttatagagg atttccagtg
    15481 aaaatctttc gctttcgtag tgtccgatat ccaccacgag catatcccgg ggtgcttcgg
    15541 catacgcgtg atatttgata tctcccgtga tgaatgcgtc agcgccggcg cgcgcggcgc
    15601 tgctgatgag gtgtgaccct gaccctccgc atacggccac ggttgaaacg gtgcgggcgg
    15661 ggtcattgct gtagcgtatg acgggcaggc cggtgagcct ccgcagccgc tccagcagcg
    15721 ccgtgcctgt cagcggagct gccagggcgc cgatggcgcc agcgccggca cccgtgtact
    15781 cattctcaag cgcaataaga tcccatgcaa cctcctcata gggatgtgct gccagcatcg
    15841 ccttcagaca ggctcccttc agatgggaag gcaagacaac ctcaatacgt acctccggct
    15901 cggtgtgcaa ctcaccaatg ctgcccgcaa acggatcagc tccctccccg gcacggtatg
    15961 tcccctcgcc tcttgcattg aagctgcagg agctatagtt tccgatattc ccggcaccgg
    16021 cggcaaagag ggcatcacgc actgccgcgg cgtgtgactc gggcacaaag gtgacaagct
    16081 tcagcagcct gtccctcagc ggctccagca ctcttatgct ctccagccct atcttttcag
    16141 ccagaatacg gcttacgcca ttgccggcgt tgtcaagagt ggtatgtgca gaataaacag
    16201 ctatgccttt cctgatcgcc gatgccacgc tggtctcgct gttgctccca taagccagcc
    16261 gcttca
//
LOCUS       DAOPBK010000042        15944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3735_length_15944_cov_5.524954, whole genome shotgun sequence.
ACCESSION   DAOPBK010000042 DAOPBK010000000
VERSION     DAOPBK010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15944
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3735_length_15944_cov_5.524954"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLA17_03965"
     CDS             <1..723
                     /locus_tag="PLA17_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84601.1"
                     /translation="VASSESGAPGNGTGARPGLIGDAESSPITSSGVGFPDTALSSEA
                     GTPGGALSTGTPGAGTFRILPPMTAFLALVNHIYKHEIKGEFQLRLWTDIYLLLERYG
                     KQILTSGLADAAEEAGIRKETVAVLTVMKQVWGVVLPEGMAVSSDAENAVVALFMNRL
                     AHPESVGSITQREMFMKNLRALKSPLKKFIFILGDIFPSIGFMKRRYNCRSKMAAFLF
                     YPHRLGKILWILGLLRTEKYDT"
     gene            1313..2782
                     /gene="cysS"
                     /locus_tag="PLA17_03970"
     CDS             1313..2782
                     /gene="cysS"
                     /locus_tag="PLA17_03970"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPK84602.1"
                     /translation="MKSALYLYNTLTRRKELFEPIAPPFVGMYVCGPTVYSEAHLGHA
                     RPAVTFDVLYRYLMHLGYNVRYVRNITDVGHLENEADDTGEDKISRRARQERLEPMEV
                     VQKYMNSFHQAMEKLNTLAPSIEPRATGHIIEQQQLTEKLIKSGYAYEVNGSVYFDVE
                     KYNSRHRYGILSGRLLDEMRAGSRALEGQDEKRGQFDFALWKKASPGHIMKWPSKWSD
                     GFPGWHLECSVMSTKYLGETFDIHGGGMDLIFPHHECEIAQSTAACGKQPVRYWVHNN
                     MVTINGQKMARSLGNFITLEEMFSGSHKLLTRAYSPMTIRFFMLQAQYRSTLDFSNEA
                     LLAASLGLQKLMNAEKTLSGLVPGGESSVDISGIAAKCYEAMSDDLNTPVAIAHLFDA
                     VRIINLVNDGKEKLSAEDIRSLQELFRIFIHDLFGLRTEESVGNDRLEGVLKLLLDIR
                     QDAKAKKDWAVSDRIRDRLNELGITVKDRKDGYDWEITA"
     gene            2779..4323
                     /gene="ispG"
                     /locus_tag="PLA17_03975"
     CDS             2779..4323
                     /gene="ispG"
                     /locus_tag="PLA17_03975"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HPK84603.1"
                     /translation="MSLTPYKSDIIQIGSLTMGGALPVRVQSMTNTDTLDTASSVSQC
                     IRIIEAGGELVRLTAQGVREAENLAAIKKGVRAAGFETPLCADIHFNPGAAEVAARIV
                     EKVRINPGNYADKRASFIRQELTDSGWMAELERTRERLMPLIKICAEYGTAVRIGVNH
                     GSLSDRIMTRYGNTPEGMAVSAIEFLKIFRGEGFNKIVVSMKSSDTLTMVMANRLLVR
                     MMIDEGMHYPIHLGITEAGEGEDGRIISAAGTGTLLAEGIGDTVRVSLSEPPEDEIPV
                     ARAIIKAVAGEACRVMNPVASLEQRKPGEKWFPQVYTREGERFMDESGEPFTGEVLTV
                     TPSGLQTMGGRQAYDRVLNPVFNYDNPEQLAIGAAALLGRFFIARHPAGLCISNSGTV
                     QGDALIRLAFSILQATEARITRNRYISCPTCGRTRFNLQEAVRKVKAATAHLTGMKIA
                     VMGCVVNGPGEMAGADYGYVGAGEGKVHIYRGTEAVIKNVPEAEAPGKLLELISSDQE
                     RRTPVN"
     gene            complement(4352..6496)
                     /gene="rnr"
                     /locus_tag="PLA17_03980"
     CDS             complement(4352..6496)
                     /gene="rnr"
                     /locus_tag="PLA17_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPK84604.1"
                     /translation="MSRKKTPKKGGIDQKKLIELIVRAMAKTPRQPLNYKQIAAKLKI
                     TEKEEKRALSEALLRMKKDGTVDERGHGKYILRAVGGYVTGTIDLTKFGYAFVITDEL
                     EDDVFIAARNLNTALHGDQVKVLLYARRKGNRPEGEVVEIISRSRATFVGSVELTKQF
                     AFLIPDNKNMPFDLFIPLSKLNGAKQGQKAVARIVEWDQRSRNPVGEIIEVLGNPGEN
                     DTEMHAILAEFGLPNRFDPGIEAAAEKIPAEITADDIAARRDFREVPTFTIDPEDAKD
                     FDDALSMRELPNGNIEVGVHIADVTHYVKPGSPIEKVARERATSVYLVDRTIPMLPER
                     LSNNICSLNPSEDKLTYSAVFEMDAKANLVNEWFGSTIINSDRRFSYSEAQKVIDTGE
                     GDMHEQLLTLNNLAQLLRRRRFASGSFSFERLEVRFNLNEEGVPLGILFREFGTANQL
                     IEEFMLLANRRVAEFVGRKLKGKTFVYRVHDKPDPEKLSSFSYFIKRFGYEIDAENIK
                     RLPAAMNKLMEEVSGKKEQNIVETLALRSMAKAVYSTDNIGHYGLAFRHYTHFTSPIR
                     RYPDMMVHRLLTKYLEGEPVHDKEKYEKLCEHSSKMERLATDAERASIKYKQVEYMSD
                     RIGEVYEGVISGVTEWGIYVEIIENQCEGMVALRELQDDYFEYDEENYCIRGRHSGKV
                     YMLGDKVSVEVVKADLQKRQLDYRLAESEDRG"
     gene            6563..6853
                     /locus_tag="PLA17_03985"
     CDS             6563..6853
                     /locus_tag="PLA17_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPK84605.1"
                     /translation="MISLSSTPSHSPSVVYRRINGEYLLIPLTDNIADMDSLYRLTET
                     GAFIWELIDGERDISDIAAKIVEEFDVEPEIAEKDALEFFREIQEFLHFRET"
     gene            6850..>7580
                     /locus_tag="PLA17_03990"
     CDS             6850..>7580
                     /locus_tag="PLA17_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84606.1"
                     /translation="MTVDLVIAGRRIRLHSREGVEIRPDERFSAFTAPAEMPFAVHRD
                     ILPETVPDTLPQEGQSLTGAGMLSEDQCHTGAGMSPENQRHTGAGMSPEDQCHTGAGM
                     SPGSTGTSSDRVTGEDHDLDADMVVDADLVADADLVVEVEPGPGEIPASAEKVFDAQL
                     MEEVPGGVINSGEPFWEIFSGGGVTYARVFLKEPERTALLEMPNGEKRWRIFTGEVTG
                     APEDVGVRVTTDSCNDRSGLAPDNF"
     assembly_gap    7581..7680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7681..8744
                     /locus_tag="PLA17_03995"
     CDS             <7681..8744
                     /locus_tag="PLA17_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84607.1"
                     /translation="VTTDSCNDRSGLAPDNFISPHLADPSQVDTGPDHPTAGTIAEAP
                     ITVNPLPYPLDGLLFYFLFSRAGDIMIHGSGVSCQGRGWLFTGRSGSGKTTLARIFDR
                     AGDRVIHDDRLVLCRSEDGWMMHNTPVYRNDEPRSAPLDHLWIIRHGSANVSEPVTGA
                     EAVAMILANSIQQNWDRVAAARLAAAADDLVSSVRVSRLSFLPDGTIREYLRLRKEEE
                     ISLAASAAGALLSAGKNITVTAGGYSMWPAIRPGDKVEIAPFVEGAAAAGRIVALRRD
                     GGFVLHRITRVMTVSGRRVIVTCGDAAARADEPAGAGMIAGIVHSVTRSGRLITPPRR
                     RWPRWVNRITAAVAGWVRR"
     gene            complement(8871..9422)
                     /locus_tag="PLA17_04000"
     CDS             complement(8871..9422)
                     /locus_tag="PLA17_04000"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK84608.1"
                     /translation="MSATGEKITHLRKSRGLTQEELAENARVNLRTVQRIESGDTEPR
                     VTTLRALCNAMNVNIEEVIETGLRENRSILFWLHLTPAAVFLLPLGNIILPLILWLSH
                     KDKTSHVREQGANIINFQILWYVVTVIIVGILMFGLKDYMATAYMLLLVLMTNVIYPV
                     FVAIRIRKGKIRNYYPAIIRFIR"
     assembly_gap    9624..9723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9828..13469
                     /gene="metH"
                     /locus_tag="PLA17_04005"
     CDS             9828..13469
                     /gene="metH"
                     /locus_tag="PLA17_04005"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPK84609.1"
                     /translation="MNRSVREALAERILVLDGAMGTMIQRHRLTEEQYRGAQYASHPR
                     SLRGNNDLLSLTQPAIIRDIHLSYLRAGADIISTNTFNANRISLSDYDMEPLVYDINV
                     AAARLAREAAEQHRAESGRESWVAGSVGPTNRTASMSPDVNDPGVRTVTFADLAEAYD
                     EQVRGLIDGGVDILLVETIFDGLNAKAALFAISGVLEERGIDVPVMVSGTITDTSGRT
                     LTGQTLGAFLITMSHYPLLSIGLNCALGAEQLKPFIEELAAKAPFHVSAHPNAGLPNQ
                     FAEYDQSPEYMGNIIRSFIRNRWVNIIGGCCGTTPDHIKVIASIAAGQKPRALPERKP
                     VTVLAGLEPLVIRPDSNFVNIGERTNVAGSLKFARLIREGNFAAALDVARDQVEGGAQ
                     VIDICMDDAMIDGVAAMRRFINIVMSEPDIARLPIMVDSSKWEVLEAGLQCIQGKPVV
                     NSISLKEGEEEFLKKARLVRRYGAAAVVMLFDEKGQAATFERRREVADRSYRLLTEKA
                     GFPPEDIIFDPNILAIATGIEEHNNYAVDYIKTTEYIKRNLPFARVSGGVSNLSFSFR
                     GNNPVREAMHAVFLYHAIKAGMDMGIVNPGLLKVYTSVEPQLLQLTEDVVLNRRKDAT
                     DRLLQYASAMKGEKQDEETKRPEWREGDVVSRIRHALVNGIDTYIEDDVEEARQQYDR
                     ALGVIEGPLMDGMNEVGDLFGSGKMFLPQVVKSARVMKRAVARLTPYIKAEIKGGETT
                     SAGKVLLATVRGDVHDIGKNIVEVILACNNYEVIDLGVMVSAEKILDTAIKENVDFIG
                     VSGLITPSLEEMVHIASLMEERGMKIPLLIGGATTSEIHTAVRIAPVYSYNTVYVKDA
                     SRAVSVMSGLAATDGSLGRSMKERYERLRHDHEASRRRKRLIALQAARANGMKTMQPM
                     PAPAMPGIINSGPVSLRTLVDYIDWTYFFFAWKINGRYPDIFNDPVKGAEAKKLYDDA
                     QVMIERIISEGLMRAEALSGIFPAERHGDDVKLITGGTASGSAAAPGETWLRFLRNQE
                     EKEPGQHNLCLADFVAEKDDHAGLFVVSVKTINPLPADIAGDDYRTIMLGILADRFAG
                     AAAEWLHREIRVKHWGYAAGEQLSMAEMFRGRYQGIRPAPGYPACPDHTGKKILFDIL
                     RVPETTGVTLTDSFAMVPVSSVCGYLFGSPGSSYFNVGTIGRDQLEDYAARCGMSVEA
                     AAKWLAPNL"
     gene            complement(13530..14792)
                     /gene="metK"
                     /locus_tag="PLA17_04010"
     CDS             complement(13530..14792)
                     /gene="metK"
                     /locus_tag="PLA17_04010"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPK84610.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALLDNFLAWDPESKVACETL
                     VTTGQVVVVGEVKTKTYIDVPTIIRNTINQIGYTKAEYRFDGNSCGILSAIHGQSPDI
                     SMGVERGKKEEQGAGDQGMMFGYAVDETENYLPLSLDIAHELVYQLAEIRREGRQMKY
                     LRPDSKSQVTIEYNDNGTPERVHTIVVSTQHDEFDKDEKKMLETIKKDVINILVPRVK
                     AQYGKEVQQLFDGKIIYHVNPTGKFVIGGPHGDSGLTGRKIIVDTYGGRGAHGGGAFS
                     GKDPSKVDRSAAYAARHIAKNMVAAGVAREMLVQVSYAIGVAEPVGIFVNTFGTSKVE
                     QSDGEIAKIIDKMFDLRPAIIEKVFDLRKPIYSETAAYGHMGRQPRTIEKTFQSFNSR
                     PDKKVTVRLFSWEELNRVNDIKQAFGLK"
     gene            complement(15221..15874)
                     /locus_tag="PLA17_04015"
     CDS             complement(15221..15874)
                     /locus_tag="PLA17_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84611.1"
                     /translation="MCKRTVMLIALLLSGLHSFSQERWIDESNGKPEVEIGYLHLYNN
                     LNADNYFKFKYRFATDNGDASLGGISLKLNIPTKLKYLDITVGSIIVKGLDLLEYGYV
                     NPQYADNHDYIINGGGIYFGVSPGIKGKILSLTTEFGIGFFSFKEYVSIVNNVTDPFA
                     DEHNLKASYGPGAISSVGLGIRIWKLGINPSVVAIYSGGAGASFTFYGFNLPLTLQF"
BASE COUNT         3670 a         3879 c         4427 g         3768 t
ORIGIN      
        1 gtagccagca gcgagtctgg cgctccaggc aatggcacag gagccaggcc cgggttgatc
       61 ggtgatgcag aatccagtcc tataaccagc agcggagtgg gcttccctga cactgcactc
      121 agcagcgaag cgggcacccc cggcggcgca ctcagcacgg gcacccccgg tgccggaaca
      181 ttccgtatcc ttcctccgat gacggcattc ctcgccctgg taaaccacat atacaagcat
      241 gagataaagg gggagtttca gttgagactc tggacagaca tatacctgtt gctggagcgg
      301 tacggcaaac agattctgac ctccgggctg gctgatgcgg cggaagaggc gggcatcagg
      361 aaggagaccg ttgcggtact cacggtaatg aagcaggtat ggggagttgt actgcctgaa
      421 ggcatggccg tcagctctga tgccgaaaat gcagtcgtgg ccctgttcat gaacaggctg
      481 gctcatccgg aatcagtcgg atcgatcact cagcgcgaga tgttcatgaa aaacctgagg
      541 gcgctgaaga gtccgctaaa aaagtttatc ttcattcttg gcgatatttt tccctccatc
      601 ggattcatga aaagaaggta taattgccga tccaaaatgg cagcttttct tttttaccca
      661 cacaggttgg gtaaaattct gtggatttta ggtcttctgc ggacagaaaa atacgacact
      721 taataaaaag tataaaaaac tgctaaatta tttcagtttt tgttggagcg aaccggatta
      781 attattaaat ttatctcagt caacttttct tttagtcaat gtcatgtctg ggcttattca
      841 gataatgggt tattgaacat aaaagacggg agatatgtgt ctgcaatttc gtttttatag
      901 gtattaatgg gcaaggcgct tcatctgcgc agtctcgctg catgaaccgt gctttgctaa
      961 tgctgccgtc atgtgtatgt cagctctgcc ccgggttcca ctggcccggg gcagtttttt
     1021 gtaagccttt tgatattgtc agcaccccca cggcagtttt tttgtgtgcc cgtcgtggac
     1081 gataactata gggtggaagt cccgaacgca ccctgacagt gagaagcctg ttgatatttc
     1141 cggcatcctg acactgtttc ctggcagtgt caatgcaatt ctcagtgcat ttcgggcacc
     1201 ctgacactgc atcatggtca tatcaatgca atgcttgatg aacttcgggc aggtggtttt
     1261 gtgacggcat aatgcggcaa aaagcattac ttttgcataa acagttgatg agatgaaaag
     1321 tgcactctac ctatacaaca cccttacacg caggaaggaa ctgtttgagc ctattgctcc
     1381 accgtttgtc ggcatgtatg tctgcggccc caccgtctat agcgaggctc atctcggcca
     1441 tgcccgtccg gcggtgacct ttgacgtgct ctacaggtac ctgatgcacc tgggctataa
     1501 tgtgaggtat gtgcgtaaca taaccgatgt ggggcatctt gaaaatgaag ctgatgacac
     1561 gggagaggac aagatcagcc gtcgtgcccg ccaggagcgc ctcgagccga tggaggtggt
     1621 tcagaagtac atgaacagct tccaccaggc aatggagaag ctgaatacac tggctccatc
     1681 aatagagccg cgcgccaccg gccacatcat agagcagcaa cagctgacag aaaagcttat
     1741 taagagtggt tacgcctatg aggtgaacgg ttcggtctat tttgacgtgg agaagtataa
     1801 cagcaggcac aggtacggta tcctttccgg acgtcttctt gatgagatgc gtgccggcag
     1861 cagggcactg gaggggcagg atgaaaagcg gggccagttt gatttcgccc tgtggaagaa
     1921 ggcttcaccg gggcatataa tgaagtggcc ctcaaaatgg agcgacggtt tcccgggctg
     1981 gcacctcgag tgctcggtga tgagtacgaa atatttagga gagaccttcg atatccacgg
     2041 tggtgggatg gatctcatct tcccgcacca tgagtgcgag attgcccaga gcaccgctgc
     2101 ctgtggaaaa cagccggtgc gatactgggt gcataacaac atggtgacaa tcaacggtca
     2161 gaagatggca cggtcactcg gcaacttcat caccctcgag gagatgttct ccggttcgca
     2221 caagctgctg acaagggcat actcgcccat gaccatccgc ttcttcatgc ttcaggctca
     2281 ataccgcagc acgctcgact tcagcaatga ggcgctgctg gctgcctcgc tggggctgca
     2341 gaaactgatg aatgcggaga aaacgttgtc cgggctcgtg cctggcgggg agtcttctgt
     2401 tgatataagc ggcatcgccg cgaagtgtta tgaggccatg agcgatgacc tcaacacacc
     2461 ggtggcaata gcacacctct tcgacgcagt caggatcatc aaccttgtca acgatggaaa
     2521 ggagaagctg tcggcagagg atatcaggtc actgcaggag ctcttccgga tcttcatcca
     2581 tgaccttttc ggcctccgca cggaagagtc agtcggcaac gacaggctgg agggtgtgct
     2641 gaaactgctc ctcgacatca ggcaggatgc aaaagcaaag aaggactggg ctgtttctga
     2701 ccgcatccgc gacaggctga acgagctggg gatcacggtc aaggaccgca aggacgggta
     2761 cgactgggag ataacggcat gagccttacc ccttataaat cagatattat acagataggc
     2821 agcctgacca tggggggtgc tctccccgta cgcgttcaga gcatgaccaa cacagacacc
     2881 cttgacacgg cttcgagtgt cagtcagtgc atcagaatca tagaggcggg aggtgagctg
     2941 gtgaggctta cggcccaggg tgtcagggag gcggagaacc ttgctgccat taagaaagga
     3001 gtcagggcgg caggatttga aacgccgttg tgcgctgata tccatttcaa tcccggcgct
     3061 gccgaggtgg cggcacggat cgttgagaag gtacgcatca acccgggcaa ctatgctgac
     3121 aaacgcgctt cattcatcag gcaggagctc actgactccg ggtggatggc agaactggaa
     3181 cgcaccaggg agaggctgat gccactaata aagatatgtg ccgaatatgg tactgccgta
     3241 cgaatagggg taaaccacgg ctcgctctct gaccggatca tgacacgcta tggcaacacc
     3301 cccgagggga tggctgtatc ggcaatagaa ttcctgaaga tattcagggg agaaggattc
     3361 aacaaaatcg tggtctcaat gaagtcgtca gacacactca cgatggttat ggccaaccgg
     3421 ctcctggtga ggatgatgat tgatgagggg atgcactacc ccatacatct gggcatcact
     3481 gaggcaggcg aaggtgagga cgggagaatc atctccgctg cagggacagg aacgctgctg
     3541 gcagagggga ttggagatac ggttcgtgtt tcgctctctg agccgcctga ggatgagatt
     3601 cctgttgccc gtgcaattat taaggctgtc gccggtgagg cgtgccgggt gatgaatccc
     3661 gttgcatcgc tggagcagcg aaaaccgggg gagaaatggt tcccgcaggt atatacccgt
     3721 gagggagagc gtttcatgga tgagtcgggt gaaccattca ccggggaggt gctgaccgtc
     3781 acaccctccg ggcttcagac catgggtggc aggcaggctt atgaccgggt tctgaatcct
     3841 gttttcaatt atgacaatcc ggaacagctg gccataggag ctgcagcgct gcttggaagg
     3901 ttttttattg cacgccatcc tgccgggctc tgcatatcaa acagtggcac cgttcaggga
     3961 gatgcactga tcaggctggc attctccatt ctgcaggcca ctgaggcaag gatcacccgt
     4021 aacaggtaca tatcatgtcc cacctgcggc cgtaccagat tcaatctgca ggaggcggtg
     4081 cgtaaggtga aggcggcaac cgcgcacctc accgggatga agattgcagt gatgggatgt
     4141 gtggtcaacg gtcccggtga aatggccgga gctgattacg gatatgttgg cgcgggagag
     4201 gggaaagttc acatctaccg gggaactgaa gccgtcatca aaaatgtgcc tgaagcagaa
     4261 gcgcccggca agcttcttga gctgataagc agtgatcagg aaagaagaac cccggtcaat
     4321 tgaccatccg gaaatcagca aacctgatac gctaacctct atcttctgat tcagccagcc
     4381 ggtaatcaag ctgcctcttt tgcaggtcag ctttcaccac ctcaacactg accttgtcgc
     4441 cgagcatata aaccttgccc gaatgccttc cgcgtatgca atagttttcc tcgtcatatt
     4501 cgaagtagtc atcctgcaac tccctgaggg caaccatccc ttcgcactgg ttctcaatga
     4561 tttcgacata gataccccac tcggtcactc cggagatgac accctcgtac acttccccga
     4621 ttctgtcact catgtactct acctgtttgt acttgattga tgccctctcg gcgtctgttg
     4681 caagacgctc catcttagag gagtgctcgc acagcttttc gtacttctcc ttatcgtgta
     4741 ccggctctcc ttccagatat tttgtcagca gtctgtgcac catcatgtca gggtaccggc
     4801 ggatgggcga ggtgaagtgt gtgtaatgcc tgaaggcgag gccatagtgg ccgatgttgt
     4861 cagtggaata gaccgctttg gccatggagc gcagggcgag ggtctcgacg atgttctgct
     4921 ccttctttcc cgagacctct tccatcagtt tgttcattgc tgccgggaga cgcttgatgt
     4981 tttcagcgtc aatctcatag ccgaaccgtt tgatgaaata actgaacgag ctcagtttct
     5041 caggatcagg cttgtcatgg acacgataca caaaggtttt tcctttcagt tttctgccga
     5101 caaattctgc cacacgcctg ttggcaagca gcataaactc ttctatcagc tggttggcgg
     5161 tgccgaactc gcgaaagaga atgcccaggg gtactccctc ttcattcaga ttgaacctga
     5221 cctcgagcct ctcaaaggag aacgagccgg aggcgaaacg cctgcgtcgc agcagctgtg
     5281 ccaggttatt gagcgtcagc aactgttcat gcatgtcacc ctcgccggtg tcaataacct
     5341 tctgggcttc actgtaagag aagcgcctgt cagaattgat gatggtgctc ccgaaccact
     5401 catttacaag gttggctttt gcatccatct caaaaacagc tgaataggtc agcttgtcct
     5461 ctgacgggtt aagtgagcag atattgtttg agagcctctc gggaagcatt ggtatggtgc
     5521 ggtcgaccag gtagacagag gttgctctct cacgggcaac tttttctatg ggtgagcccg
     5581 gtttgacata atgggtgacg tcggcgatat gcacaccaac ctcgatgtta ccgttgggca
     5641 actccctcat cgagagggca tcgtcaaagt ccttggcatc ctccgggtca atagtgaagg
     5701 tcgggacttc gcggaaatcc cgcctcgcgg cgatatcatc agcggtaatc tcagcgggaa
     5761 ttttttctgc tgcagcctca attccgggat caaacctgtt gggcaggccg aactcagcca
     5821 ggattgcatg catctccgta tcattctcgc cggggttgcc gagaacctca attatctcac
     5881 ccacagggtt tcgcgagcgc tggtcccact ccactatgcg tgccacggcc ttctgtccct
     5941 gcttcgctcc gttgagtttt gagagaggaa taaagaggtc gaacggcatg ttcttgttgt
     6001 cgggtatgag gaatgcgaac tgtttcgtta actctaccga gccgacaaag gtggctcgtg
     6061 aacggctgat aatctcaacc acttcgccct cggggcggtt ccccttgcgg cgtgcataga
     6121 gcagcacctt cacctggtcg ccatggaggg cggtattaag gtttcgcgct gcgatgaata
     6181 catcatcctc cagctcatcg gtgatcacaa aagcgtatcc gaatttggtg aggtcaattg
     6241 tgccggttac ataaccgcct acagccctga ggatatattt gccatggccc cgttcatcga
     6301 cggtgccgtc cttcttcatc ctgagaagag cttcggagag cgcccgcttc tcctcctttt
     6361 ctgttatctt taattttgcg gctatctgtt tgtagtttaa cggttgtctc ggggttttag
     6421 ccatcgccct gacaattagc tcaataagtt ttttttgatc aatcccgcct tttttaggtg
     6481 ttttttttct agacatttct gctttgttta gcgcttccaa agatagattg aaattggcta
     6541 tctttgaatt gattatacga ttatgatttc actctcatct accccctcgc attcgccctc
     6601 ggtggtgtac cgcaggatca atggcgagta cctgctcatt ccactgacgg acaatattgc
     6661 cgacatggat tctctgtaca ggctaaccga gacaggtgct tttatctggg agttgattga
     6721 cggagagcga gacatcagtg atatagcggc aaagatagtg gaagagttcg atgtagaacc
     6781 tgagattgcc gaaaaagatg ccctggaatt tttcagggag attcaggaat tccttcattt
     6841 cagggagacg tgacggttga ccttgtcata gccgggcggc ggatacggct tcactcccgt
     6901 gagggtgtgg agattcgtcc tgatgagcgt ttcagtgcgt tcacggcgcc ggctgagatg
     6961 ccgtttgcgg tacatcgtga cattttgcct gagactgtgc cggacactct gcctcaggag
     7021 gggcaatctc ttacaggtgc cggaatgctt tcagaggatc agtgccatac aggtgccgga
     7081 atgtctccgg agaatcagcg tcatacgggt gccggaatgt ctccggagga tcagtgccat
     7141 acaggtgccg gaatgtctcc ggggagcacc ggaacttctt ctgaccgggt gacaggggaa
     7201 gatcatgatc tggatgctga catggttgtt gatgctgatt tggtcgctga tgctgatctg
     7261 gttgttgagg ttgagcccgg gcccggggag attcctgctt cagcagagaa ggtgtttgat
     7321 gcgcagctta tggaggaggt gcctggcggg gtaatcaatt caggcgaacc attctgggag
     7381 attttttcgg gaggaggtgt cacctatgcg cgcgtgttcc tgaaggagcc ggagaggacg
     7441 gctcttctcg aaatgcctaa cggcgaaaag cggtggcgga tatttacggg tgaggtgacc
     7501 ggcgctccgg aggatgttgg cgtcagggtc acgacagatt cttgcaatga caggtcaggt
     7561 ttggcaccgg acaatttcat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 gggtcacgac agattcttgc aatgacaggt caggtttggc accggacaat ttcatctcac
     7741 cgcaccttgc agatccatct caggttgata ccggcccaga ccatccaaca gccgggacta
     7801 ttgctgaagc acctattact gtcaatcctc ttccatatcc tctagacggg ttattatttt
     7861 actttctctt ctcccgtgcc ggagatatca tgatccacgg ctcaggggtg tcatgccagg
     7921 gccggggatg gctcttcacc ggccgttccg gcagcggcaa gaccaccctg gcacggatat
     7981 ttgaccgggc aggcgacagg gtcattcatg acgacaggct ggtgctgtgc cgcagtgagg
     8041 acggatggat gatgcacaac acacctgttt accgcaatga tgaaccccgg tctgctcctc
     8101 ttgaccacct gtggataata aggcatggct cagccaatgt gtcggagcct gtcaccgggg
     8161 cagaggctgt ggcaatgatt cttgccaact ctatccagca gaactgggac agggtagcgg
     8221 cagcaaggct tgccgctgca gctgatgacc tggtatcgtc ggtacgcgtg agcaggctct
     8281 cgttcctgcc tgacggaacc attcgtgagt acctgcgtct gaggaaggag gaggagattt
     8341 cacttgctgc aagcgctgcc ggggctctac tgagtgcagg caaaaacatt actgtcactg
     8401 cagggggtta cagcatgtgg cctgccatca gaccgggtga taaggtggag atagctccat
     8461 ttgttgaggg ggctgcggcg gcggggcgga tagtggctct gcgtcgcgac ggcggcttcg
     8521 ttttgcacag gattacccgg gtgatgaccg tttccggacg cagggtgata gtcacctgcg
     8581 gtgacgccgc cgcgcgtgct gatgagcctg ccggagcagg gatgatcgcc ggcattgttc
     8641 actcagtcac ccgctccggc agactcatta ccccgccccg ccgccgctgg ccgcggtggg
     8701 tgaaccggat aacggccgct gttgccgggt gggtgagaag gtgactccgc tgttataatt
     8761 cctgaacccc tgcagcgttc cggttggggc ggcctcgcgg ataaagcatt gttccgtcat
     8821 tgtttcatgc aacaacgaga ggggaactat acatcctggt cttgaaaaga ctacctgatg
     8881 aaccttataa tagcgggata ataatttctg atcttcccct ttcttatccg gatggcgaca
     8941 aaaaccggat aaataacatt tgtcatcagg acgagaagca gcatgtatgc ggttgccata
     9001 taatctttca gtccgaacat aagaatgccg actatgatga cagttactac ataccagaga
     9061 atctgaaaat taataatgtt ggctccctgc tctctgacat gtgacgtctt gtccttgtgc
     9121 gacaaccata aaatcagtgg cagaataatg ttaccgaggg gaagcagaaa gactgcagca
     9181 ggtgtaaggt gaagccagaa caggatgctc ctgttttcac ggaggccagt ctcaatcacc
     9241 tcttcgatat ttacattcat ggcattgcat aatgccctca gggtggtgac cctcggctcc
     9301 gtgtcaccgc tctcgatgcg ctggactgta cgcaggttca cccttgcatt ttcggcaagc
     9361 tcctcctggg tgaggccccg ggattttcta agatgagtga ttttttctcc tgtggcagac
     9421 atatcccaac tatttgtatg cgaaaggtaa tgaaaatgtt ctggcagtgt tgcggcattc
     9481 atgcggcatt ggctgacatt taagcggcat ttgtgccgga attgcaggat caggggtcca
     9541 ttccggctgg cagcatcaat cacccaccag gcacctgcca cggagtatgc cccgtaagat
     9601 tgcctttgtt gccgggtggg tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnntggggcg gcctcgcggt gattcaaccg gatgacagtc actacacaga ctggttttca
     9781 tatatcgcgg ctttaagtta gctttgacaa actataatcg ccagacattg aatagatctg
     9841 tcagagaggc actggctgag aggatactgg tcctcgacgg agccatggga acaatgatac
     9901 agcgccacag acttaccgag gagcagtacc gcggtgcgca gtatgccagc cacccccggt
     9961 cactcagggg caacaacgac ctgctgtcgc tgacacagcc cgccataatc cgtgatattc
    10021 acctgagcta cctgagggca ggcgctgaca taatcagcac caataccttc aatgcaaaca
    10081 ggatatcgct gtcagactac gacatggagc cgctcgttta tgatataaac gtggctgccg
    10141 cacggctggc cagggaggct gctgagcaac accgtgcaga aagtggccgc gagtcgtggg
    10201 tggccggctc cgttggtccg acaaaccgta cggcctcaat gtcgcccgat gtgaatgacc
    10261 caggcgtacg gacagtaaca tttgcagatc ttgcagaggc ctatgacgaa caggtgcgcg
    10321 gactgattga cggcggcgtg gatatcctgc ttgttgagac catctttgac ggcctcaatg
    10381 ccaaggcagc actgtttgca ataagcggcg tactggagga gagaggtatt gatgtgccgg
    10441 taatggtctc aggcaccatc actgatacca gcggccgtac actcacaggg cagaccctcg
    10501 gggcctttct cattacaatg tcgcactacc cgctgctgag catagggctc aattgtgcac
    10561 tgggggcaga gcagcttaaa cctttcatag aggagcttgc ggcgaaggcg ccattccatg
    10621 tttcggcaca ccccaatgcc ggtctgccaa accagtttgc tgagtatgac cagtcgcccg
    10681 aatacatggg caatatcata aggagtttca tccggaacag gtgggtgaac atcatcggag
    10741 gctgctgcgg caccaccccg gaccatatta aggtcattgc ctccattgcc gcaggacaga
    10801 aaccgagggc gcttccggaa aggaaacccg tcacggtact tgctggtctc gagccgctgg
    10861 tgattcgtcc tgacagcaac tttgtcaaca ttggagagcg gacaaacgtg gccggttcgt
    10921 taaagttcgc gcggctgata cgcgagggga acttcgcggc agcgctcgat gtggcgcgtg
    10981 atcaggtgga gggcggcgca caggtgatag atatatgtat ggatgatgcc atgattgatg
    11041 gcgtcgcagc catgagacgg ttcatcaaca tagtgatgag cgagcctgac atagcaaggc
    11101 tgcctataat ggttgactcc tcgaagtggg aggtgctgga ggcaggactg cagtgcatac
    11161 agggcaagcc ggttgtcaac tccatcagcc tgaaggaggg tgaggaggaa ttcctgaaga
    11221 aagcacggct ggtgaggcgt tacggcgctg ccgcagtggt gatgctcttc gatgagaagg
    11281 gacaggctgc cacattcgag agacgcaggg aggtggctga cagatcgtac cgtctgctga
    11341 cggagaaagc cggtttcccg ccggaggata tcatcttcga tcctaatatc ctggctatcg
    11401 ccacaggcat tgaggagcac aataactacg ctgttgatta cataaaaacc actgaatata
    11461 ttaaaagaaa cctcccgttt gcaagggtaa gcggcggagt gagcaacctg tcgttttcgt
    11521 tccgcggcaa taacccggtc agggaggcga tgcacgcggt gttcctctat cacgccatta
    11581 aggcaggcat ggatatgggg attgtcaatc ccggactgct gaaggtctat acctccgtgg
    11641 aaccacagct gctgcaattg acagaggatg tggtgttgaa ccgacggaag gatgctaccg
    11701 acaggctgct gcagtatgct tcggcgatga agggggagaa gcaggatgag gagacaaagc
    11761 gcccggaatg gcgcgaaggc gatgtggtga gccgtatcag gcatgctctt gtgaacggta
    11821 tagacacata catagaggat gatgttgagg aggcccggca gcagtatgac agggcactgg
    11881 gagtcatcga gggtccgctg atggatggaa tgaacgaggt gggtgacctg ttcgggtcgg
    11941 gaaagatgtt cctgccgcag gtggtcaaga gcgccagggt gatgaagagg gcagttgcac
    12001 ggctgacacc ttatatcaag gctgagataa aggggggaga aaccacctct gcaggcaagg
    12061 tgttgctggc aacagtcagg ggcgacgttc atgacatcgg gaaaaatatt gttgaggtca
    12121 ttttggcgtg caataattat gaggtgatag acctcggcgt gatggtgtcc gcggaaaaga
    12181 tacttgatac agccataaag gagaatgttg atttcatcgg tgtatcaggg ctgatcaccc
    12241 cgtcgcttga ggagatggtg catatagcat cgctcatgga ggagaggggg atgaagatac
    12301 cgctcctgat aggcggggca acaacctcgg agatacatac agcggtcagg attgcgcctg
    12361 tttactcgta taacactgta tatgtgaaag atgcgtcgcg ggctgtctct gtgatgtcag
    12421 gtctggcggc tactgacggc tctctcggca ggagcatgaa ggagaggtat gaaaggctgc
    12481 gtcatgacca tgaggcatcg cggaggcgga agaggctcat agcactgcag gcggccaggg
    12541 caaacggcat gaagaccatg cagccaatgc ctgcaccggc aatgccggga ataataaact
    12601 ccggtcccgt gagcctgcgg acactggttg attacataga ctggacctac ttcttcttcg
    12661 cgtggaagat caacggcagg tatcctgata tcttcaatga tcctgtaaag ggggcagaag
    12721 cgaagaaact ttatgatgat gcacaggtga tgattgaacg gattatcagc gagggattga
    12781 tgagagctga ggcgctctcg gggatcttcc cggcagagcg gcatggagat gacgtgaagc
    12841 tgatcaccgg aggcacggca tcgggaagtg ctgccgctcc cggtgaaacc tggcttcgtt
    12901 tcctgcgcaa ccaggaggag aaggagccgg gccagcataa cctttgcctc gcagacttcg
    12961 tcgcagaaaa agatgatcac gccggactct tcgtggtgtc agtgaagacc atcaatccgt
    13021 tgccggcaga tattgcggga gacgactacc gtaccattat gctgggaata ctcgctgacc
    13081 gctttgccgg ggcggcagcc gaatggcttc atcgcgagat acgcgttaaa cactggggtt
    13141 atgcagcagg tgagcagctt agcatggctg agatgttcag gggcaggtac cagggtatca
    13201 ggccggcgcc gggctaccct gcatgccccg atcataccgg caagaagata ctgtttgata
    13261 ttctcagggt gccggagacg acaggtgtca cgctgacgga tagttttgcg atggtgcctg
    13321 tctcttcggt ttgtggttat ctgtttggat cgcccgggtc atcatacttc aacgtgggaa
    13381 ccatcgggcg cgaccagctt gaggattatg ccgccaggtg cggcatgagc gttgaggcag
    13441 cggcaaaatg gctggctccg aatctttaga tttgcatcat ggaacagacg accgtaatag
    13501 aacttataaa gaactctgaa aagttgcttt tactttagcc cgaaagcttg ctttatgtca
    13561 ttgaccctat tgagttcttc ccaactgaag agccggacgg ttaccttctt gtcaggtctg
    13621 ctgttgaatg actgaaaggt tttctcaatg gttcgcggct ggcggcccat gtgtccgtaa
    13681 gctgccgtct cactgtagat tggcttgcgc aggtcaaata ccttctcaat gattgccggc
    13741 cggaggtcga acatcttgtc aatgatcttt gcaatctcac cgtcgctctg ctccaccttc
    13801 gaggtaccga aagtattcac gaagatgcca accggctcgg ccacgccgat ggcgtatgag
    13861 acctggacaa gcatctcgcg ggcaacaccg gcagcaacca tgttcttggc tatatggcgt
    13921 gctgcgtatg cagccgaacg gtcaaccttc gaggggtctt tgcccgagaa ggcgccgccg
    13981 ccatgagcac cacggccgcc atacgtgtca acgattatct ttctgcctgt caggccgctg
    14041 tcgccgtgag gcccgccgat aacgaatttg ccggtgggat tgacatgata tatgattttc
    14101 ccgtcaaaga gctgctgcac ctctttgccg tactgagcct taacccttgg aacgaggatg
    14161 tttatcacat cctttttgat cgtctccagc atcttcttct cgtccttgtc aaattcatca
    14221 tgctgggtgg aaacgactat cgtgtgcact ctctctgggg tgccgttgtc gttatactca
    14281 attgtgacct gactcttcga gtcgggcctg aggtatttca tctgcctgcc ttcgcgacgg
    14341 atctcagcga gctggtaaac gagttcatgg gcaatatcga gagagagagg caggtagttt
    14401 tctgtctcgt caacggcgta tccgaacatc attccctggt cgcctgcacc ctgctcttcc
    14461 ttcttcccgc gctcaacgcc catagagata tcgggcgact gtccatgaat ggcagacagg
    14521 attccgcatg agttgccgtc gaacctgtac tcagcctttg tgtagccgat ctggttgatg
    14581 gtatttcgga taattgtcgg tacgtctatg tatgtttttg tcttgacctc gcccacaaca
    14641 acaacctggc ctgtggtgac gagagtttcg catgccacct ttgattcggg gtcccatgcc
    14701 aggaagttat caagaagtgc gtcggagatc tggtcggcaa ccttgtcagg gtgcccctcg
    14761 gaaacggatt cggaagtaaa taaatagctc atctgttaat cttattaagt ttaacaatgg
    14821 caggggcgga ttgttgagaa agcagcggat ggataccggt ttagcatttt tttccgtggt
    14881 tgcaatcagt gcaaatctgt ccacctgtcg tataattggg gtcaaagata tatattaaaa
    14941 ggaaatgaac tcgagtttgg ccgggatatt tatttaagag ttagaactcc aatattttca
    15001 tctcagttat cggtggctaa tagtatagac aaagctacag atgctctgtt ctgcaaataa
    15061 ccaatgaaat gataatttct gagggctgca ggctatcttc cgttcagctg cctgaaggtt
    15121 tcttcgaggc gggcgccggc taagcgtgtc atacgttatt ccgtatttaa ctgaagatca
    15181 ggaacagtca ggcctgctgc ctgactgttc ctgaaacaat tcagaattgc aaagtaagcg
    15241 gcaggttgaa gccatagaac gtgaagcttg cccctgcgcc tcctgagtag atagcaacga
    15301 cggatggatt aatccccagt ttccagattc ttatgcccag acctacagag ctgatagctc
    15361 ccgggccgta tgatgctttc aggttatgct cgtctgcgaa cgggtcggtg acattgttta
    15421 ctatgctcac atattccttg aacgaaaaga accctattcc aaactctgtg gtaaggctga
    15481 ggattttgcc tttaattccg gggctgacac caaaatagat accgcctccg ttgattatgt
    15541 aatcatggtt atccgcatac tgcggattta cataaccata ctccaacaaa tccagacctt
    15601 tcacaattat agacccaacg gtaatatcca ggtatttgag tttggtcggg atatttagtt
    15661 taagactaat gcctccaaga cttgcatctc cgttatcagt ggcaaatcta tatttgaatt
    15721 tgaaatagtt atctgcattc agattattgt aaagatgcaa atacccgatt tcaacttcag
    15781 gtttaccatt gctctcgtca atccatctct cctgggagaa agagtggagt cctgacagaa
    15841 gcagggcgat tagcataact gttcgtttac acatggccaa ccatattttg tttgcagcta
    15901 aaagttatta aatcaaacac ttacattcaa ttctcctatt attc
//
LOCUS       DAOPBK010000043        15909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3749_length_15909_cov_6.752113, whole genome shotgun sequence.
ACCESSION   DAOPBK010000043 DAOPBK010000000
VERSION     DAOPBK010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3749_length_15909_cov_6.752113"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2429
                     /locus_tag="PLA17_04020"
     CDS             <1..2429
                     /locus_tag="PLA17_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK84612.1"
                     /translation="TGSATCIECHKKEYDLWKGSHHDLAMDIATDSTVLGDFNDFKFR
                     HRGEVHRFFRKGEKFFVNTEGVNGRFEDFEVAYTLGYTPLQQYLVPFEGGRLQCLPIA
                     WDTGQGRWFHLGDTLYTDEEAGPGNWLHWTRQAQNWNGMCADCHTTNLKKNYNPVTRT
                     YNTTWSEIDVSCEACHGPASEHLAWAALPEGSRPADVNTGLIVRTRDLNNEELLNVCA
                     RCHSRRAVMGDYTDGNGDLLNYMIPMLTVRPLYHTDGQILDEVYEYGSFTQGRMYEKG
                     VRCSDCHDSHSVRTIEPDNRLCLQCHRPDIYDTPKHHFHKMDPGSVAGPGSIVGQGSI
                     VGQGNIAGPGSVVEPGSVVEPGSVVEPGSVVDPWSVEGQWSVVRQENVVRPGSVAGPG
                     DDKGIKGGAGITGVRRLINRGEPQYVEGTGAQCVNCHMTGRYYMGNDYRRDHSFRIPR
                     PDLTAATGVPNACNDCHKDKTTAWSQSYILKWYGERMRPHYGETFAAAEKGDRSVIPD
                     LILYSENELFPLMVRATAVRFLGRFNTPESNRAVELALTDPAPLVRHTAVMSYNPTDI
                     MSYEKLMLPLLNDPVRGVRSEAAIRLSEVREERLSLPARKARRAALDEYMRINLYNAD
                     FPGGLFNLGVMYANAGDLARAEAAYRDALGIDDQFYLAKVNLATLFAQQGRPGEAEKL
                     LRDLLRDHPELHELNSSLAMLLAEQGRYGESREFFLKAAGLMPEQTRILYNLGLLENK
                     LGNTISAEKYLTEALNRDPGNYDYLYALATFCLEHGKKERALTYASRMAARYPGDQAA
                     RQLLRAAGKN"
     gene            complement(2456..5791)
                     /locus_tag="PLA17_04025"
     CDS             complement(2456..5791)
                     /locus_tag="PLA17_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPK84613.1"
                     /translation="MKNNQCHSGRAIKHAGLLMVLLLAPVFTAGAQDPFMQYPDIQGN
                     TIVFASGGDLWKVAAEGGTACRLTFSDGRESMPEISPDGSLIAFTGEYDGNTDVYVMN
                     IDGGNITRLTWHPGADEVVGWNAAKNKIMFTSGRNSPSRYVKMYLVSPDGTGLEEMIM
                     YDAARGSFSPDGMKIAYNKDSQDNATWKRYRGGRAQEVYIYDLETNKETNISNYEGSD
                     RWPMWIGNKIYFSSDRDRVLNIWSYDTGSGKIEQVTRHTEYDVRHPDYGGNQIVYELG
                     GDIWKLDLTTGVSARVPVKILTDMEERRPYMKDVSRNITGVNISPSGKRALVVARGDI
                     FTLPEKEGPVRNLTASSGARDKDAVWSPDGSRIAFFSDASGEYELYVMKPDGKEEPLK
                     LTSLGAGYRHTLKWSPDSKKIAWTDQTLTLWYIDVATKAITKVDKEEYENVDVSLDLK
                     SIFDYSWSPDSRYIVYSKMNEDYMYQLYVYGLETKSINGISNGLFHDFNPVFTNDGEY
                     ILFISNRRFSPTYCDLEWEMVYQKVAGVYAVTLRKDGKSLMPWRSDEEPVSSTVAPVS
                     TGTVDAGKGQAGRSAGKTAVAAAPASPLMVKIDFDGITDRVEALPVAKGNYRNLAVND
                     RSIFWLNSDEGNFNRFEVSGHGPMNLFSWSLKSGKASQIAEGIDDYRLSADGSTIVLR
                     KDGGVSLMPADGGNPSPLRLTDMKVWYDPVSEWKQIFNEAWRMERDYYYEPGMHGQDW
                     PALKLKYEKLADRATCRQDLTFIIGEMIAELNTSHTYVYGGDAKRRSEPVNTGMLGAD
                     YSVDKQNNFYRFSKIFREKDWSREAWPPLAKPGVNITEGDYLLKVNNNEVKADREVYS
                     YFVGLAGKQVTLTVNSKPTLTGAREVTVVPSDSETRFRYMDWLESNRMTVDKASGGKI
                     GYIYLPDTWNGSATDFPRYFYSQTKKEGLIIDGRFNGGGLDPEIFLQRLQKKPHGYWT
                     RRQSADQPIPHLAVNAHMALITNRYAGSGGDELPYEFRWFGMGPVIGTRTWGGLVGVS
                     QFIELIDGGSITAPDYRIYNEKGEWVVENEGVTPDIIIDIDSKKYSEGYDTQLMKAVE
                     VVMKKISEEPRSFPQHKPYPVDR"
     gene            complement(5814..6089)
                     /locus_tag="PLA17_04030"
     CDS             complement(5814..6089)
                     /locus_tag="PLA17_04030"
                     /inference="COORDINATES: protein motif:HMM:NF009508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HPK84614.1"
                     /translation="MFYILFYKTVENYIERRAPFRDEHLGLVHEFRNDGRLFMAGALA
                     EPSDGAVLVFRGDTPQAAHDFADRDPYVRNGLITEWSVRPWMVVTGQ"
     gene            6341..7675
                     /locus_tag="PLA17_04035"
     CDS             6341..7675
                     /locus_tag="PLA17_04035"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK84615.1"
                     /translation="MRRKSTYSGSIPLKISIIFRSLRHRNYRLFFSGQSISLIGTWMQ
                     RIALPWVVYRMTGSEVLLGIVGFAGSIPSFLLAPFAGVLIDRWSRYRVLLLTQIISML
                     QAGVLAWLSLSGSLEIWHILVLSVALGCINSFDMPARHSFVIDMVNGKEDIGNAIALN
                     SMMFNGARLIGPSVAGIVLASVGEGACFLINALSYIFVIASLMMMRVTETREKKEPVP
                     MFREMKEGWDYVFGFAPIKHIILLLALVSLMGASYQVLMPVYAKDILQGDSHTYGFLM
                     GGAGAGALLGAIYLASRNTVLRLGRLIPAATALLSTGLIAISISKSFFISMFLIFFTG
                     LGMMAHTAASNTVLQTIADDDKRGRVMSFYTMALMGTAPFGSLLVGWMAKVLGTPWTI
                     FIGGAACLLGALVFYRRLPELKRLVRPVYVRMGILPEVAKGIRTASEPGSGV"
     gene            7831..9147
                     /locus_tag="PLA17_04040"
     CDS             7831..9147
                     /locus_tag="PLA17_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK84616.1"
                     /translation="MRTKRSIAVAAALIIGCTAVTAQAKQWSLTDCIGYALEQNIQVR
                     KAGVSVSLGELNLQQSKDNMLPSLSASLSENLGWRQETNAPDETSWAGSSRTNASLSS
                     GLTLFNGFQLKNQVRQAGLDLKSLQYSADQTMESISLNIMSAYLQVLYSEEQVTNAGN
                     QAEVTREELALAAERLSLGAIANSDYLQVKAQLASEESSLASAANSLKMARLNLMQLM
                     EYPVDEAFEIIRPDIDAIISTAPATDAVSLYEEALRVKPQVKIASLNRENASIDLEIA
                     RGGFLPSLSLNAGMSTGFTGATEMGAQLKSGFSPSVGLSASIPIFRNNQTRASVTRAK
                     YGITTAELDELNTRNQLRKEVEQAVLDAGSALINYQAALKRYDSSVENYNVSEEKFKL
                     GAMNSVDFLIQKTNLTAAESNLLQAKYELVYSHKIIDFYRGVELTF"
     gene            9172..10584
                     /locus_tag="PLA17_04045"
     CDS             9172..10584
                     /locus_tag="PLA17_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK84617.1"
                     /translation="MMKKSNVFISAALLTVAVATLATVHSCRKSNTYRYETVKVTRGS
                     IVNEVTATGTLEAITSVVVGTQVSGIVEKLYVDFNSPVKKGQILAELDKTALRSSVQQ
                     SLASLENSKAEMEYQASNYKRSKALREKNLIAEADYDQAKYNYDRSVATLKNSQAQYD
                     KALVQLGYATIYSPIDGVVMNRAVDEGQTVAASFNTPEIFTIAQDLTQMQVEADINES
                     DIGIVRVGQRVEFDVDAYTGEKFTGTVEQIRLAPVTTSTVVTYTVIINAPNPDQKLLP
                     GMTANIVIYAEEHLDVLTLPYKAVKFKPDAEYLARMAEDFAAGKAPGTMNAKAGSGAF
                     PGAGSGPLPGGGSRPLPGAGSGAFPGAGSGTFPGTGAGPFPGTGTGEGFEMPAMVWIR
                     AGEGIQPVPVKLGSNDGVNAEVISGLAEGDEVIVKMTVATAKEKKEKKAASNPFMPGP
                     PGRRNTQGGSRAGTDSSTKR"
     gene            10587..11327
                     /locus_tag="PLA17_04050"
     CDS             10587..11327
                     /locus_tag="PLA17_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK84618.1"
                     /translation="MKTIIDIANLKKDYHVGEVTVHALRGVDLKINEGEFVAIMGASG
                     SGKSTMLNIIGCLDRPTDGTYLLDNVNVAGMSKNERAAIRNLKIGFVFQSYNLLSRTS
                     ALENVELPLMYNRSVSSRERREKAIAALEAVGLADRMNHMPNQLSGGQQQRVAIARSL
                     VNDPVVILADEATGNLDTRTSYEIMSLFQELNNKGKTIVFVTHEPDIAKCMTRSVVFR
                     DGSIIREEDVKNRLNAAEVLRTLPVEQD"
     gene            11339..12559
                     /locus_tag="PLA17_04055"
     CDS             11339..12559
                     /locus_tag="PLA17_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK84619.1"
                     /translation="MNPGNLLKIAIKALKRNKMRSFLTMLGIIIGVASVITMLAIGQG
                     SKKSIQDQIAGMGSNMLFVMPGNMRMGGVQQGSSSSQRLTVADVKAIKTECDAIVAVS
                     PEVRSSGQAVSGNSNWPTTIYGGNEQYLEIRSWDVVSGRNITDTEAESSAKVCLVGRT
                     VADELFGEGIDPTGETIRFQSIPFKIIGVLSKKGQNSFGQDQDDVIIAPYTTVQKRIL
                     AQTHIQSIQMSARSAEESELAQSQVEEVLRRTHKLRENEDNDFEVRSQEELATTMTSV
                     TEILTILLGAIAGISLLVGGIGIMNIMYVSVTERTREIGLRMSVGGRSIDILLQFLIE
                     SILLSVFGGLIGIVIGFGASAIVEALTSWPISVMWSSVILAFAVCTVIGIFFGWYPAR
                     RAADLDPIDALRFE"
     gene            12606..13679
                     /locus_tag="PLA17_04060"
     CDS             12606..13679
                     /locus_tag="PLA17_04060"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPK84620.1"
                     /translation="MAKRRRGLPVLLHATGWIILFILPQFLISGGLFNDARTTRVVLF
                     NTLVFLILFYTNYFWLVPRLAQKKKWLAFLVLAASLIVLLGYLSGKYYENLFAPPREV
                     RERIREVADHRPQGDNRMPRRGGGMVLYNFFITSFLVSGFAVGIRYAESAVRKEEEIK
                     ELEREKLNSELALLKNQVSPHFLFNTLNNIYSLIGINRDDARDAVLSLSKMMRYMLYE
                     SEQGNTRLSNEIAFMKGYIDLMKLRLSDRVKLTVSFPEDYEDQDVPPLLFIPFIENAF
                     KHGVSAAGNSFIDIALGVEEGMITFIAANSIGGRVSDGPQVASGIGLDNVRKRLALLY
                     PGRHDLRIDRQESVFTVKLTIRS"
     gene            13692..14405
                     /locus_tag="PLA17_04065"
     CDS             13692..14405
                     /locus_tag="PLA17_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPK84621.1"
                     /translation="MTRVVAIDDEPLALQLVKGYVEKTPTLELAGAFDNPVDAVAFIR
                     SGDVDLVLLDIQMPDLTGTELARVIAGGPKVIFTTAYEKYALEGFRLDAVDYLLKPFS
                     YAEFLKAVQKAEKLINLERKELPSLEIRNEFLFIRSDYKIRRIDFSEIHYIEGLKDYV
                     KIFLRGEKKPLLSQSTLKALESRLPEDRFMRVHRSYIVNLETVKVIDRGRIVYGEVRI
                     PVTEQYRDRFQEFLDRNFI"
     gene            14669..15217
                     /locus_tag="PLA17_04070"
     CDS             14669..15217
                     /locus_tag="PLA17_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84622.1"
                     /translation="MKTRFALLMILALVAVMFSGCEKEKEDPSVTLGAQANATVPGFY
                     SVSLDKTYTMSQAAASPGVIDILCFYEAEGGNNISLASPGSGIKEIFTGDDRPDTWSI
                     KNTTFFFQTALTASQFEAVQDGDALIETSFDADNGRKKAKDVQVGQVWAFKTQDGTYG
                     LLLATAVTQGTDGTVTFKLRTK"
     gene            15225..>15909
                     /locus_tag="PLA17_04075"
     CDS             15225..>15909
                     /locus_tag="PLA17_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84623.1"
                     /translation="MAKRIQCIALLIIIVFAGCVREDPETTPLILLKTGAAFTADGSE
                     VPPGGVLRFGLSVSGGGGAITNLVVKRISDGVAVTEADKGMYISYGGLDTTLIYTKSY
                     AQTERWVFSVMNSYRDTASVSLTVLKGAGSAWGEINYHPSIKAGLQDNQTLPHFVDLH
                     SGAVWDAENVTGHEAEVDMAAFWYITSGTSSPTLTCPAYSSALTYYPLFGSWDVRNQT
                     MYDYYTSDND"
BASE COUNT         3863 a         3950 c         4351 g         3745 t
ORIGIN      
        1 tcacaggctc tgccacctgc atcgagtgcc ataagaagga gtatgacctc tggaaaggat
       61 cacaccatga tctggcgatg gacatcgcaa cggacagcac cgttcttggt gatttcaatg
      121 attttaagtt caggcacagg ggagaggttc accgtttctt ccgcaagggc gaaaaattct
      181 tcgtcaacac cgaaggagtc aacggcagat tcgaggattt tgaggtagcc tatacgctgg
      241 ggtacactcc cctgcaacag tatctggtac cgttcgaagg aggacgtttg cagtgcctgc
      301 cgattgcatg ggataccggg caggggagat ggtttcacct gggtgacacc ctttacactg
      361 atgaggaggc agggcccggc aactggctgc attggacccg gcaggctcag aactggaacg
      421 gcatgtgtgc cgactgccac actaccaacc tgaagaagaa ctacaacccg gtcaccagaa
      481 catacaacac cacctggtca gagatcgatg tcagctgcga ggcatgtcac ggacccgcct
      541 cggaacacct ggcatgggca gcactaccgg agggctcgag gccggcggat gtcaatacgg
      601 ggctgattgt ccggacccgc gatctgaata acgaagagct gctgaacgtg tgcgcacggt
      661 gtcactcgcg cagggcggta atgggagact atacagacgg caacggcgac ctgctcaatt
      721 acatgattcc gatgctgacg gttcggccac tgtatcatac agatggacag atacttgatg
      781 aggtttatga atatggctcc ttcacccagg gcaggatgta tgaaaagggt gtcaggtgca
      841 gcgactgcca tgactcgcac tctgtcagaa ccattgagcc ggacaaccgg ctctgccttc
      901 agtgccaccg tccggatatc tatgacaccc cgaagcatca cttccacaag atggatccgg
      961 ggagtgttgc gggtccgggg agcattgtgg gacaggggag cattgtggga caggggaaca
     1021 ttgcgggacc ggggagcgtt gtggaacctg ggagtgttgt ggaacctggg agtgttgtgg
     1081 aacctgggag tgttgtggat ccgtggagcg ttgaggggca gtggagcgtt gtgaggcagg
     1141 agaacgtcgt aaggccgggg agcgttgcgg ggccgggaga cgacaaaggt ataaaaggtg
     1201 gtgcaggaat tacaggcgtc cgcaggctta ttaaccgcgg agaacctcaa tatgtcgagg
     1261 gcaccggtgc acagtgcgtc aactgccaca tgaccggcag gtattatatg ggcaacgatt
     1321 accgtcgtga ccacagcttc aggattcccc gtcccgacct gacggcagcg acaggtgttc
     1381 ccaatgcctg caacgactgc cacaaggata aaaccacagc ttggtcacag agttacatcc
     1441 tgaagtggta tggtgagcgc atgaggcctc attacggaga gaccttcgcg gcagcggaaa
     1501 aaggagaccg gagcgtaata cctgatctga tactctattc ggagaatgag cttttcccgc
     1561 tgatggtgag ggctacggca gtcaggttcc tcggacggtt caacaccccg gagagcaatc
     1621 gtgccgttga gctggcgctg acagatccgg ccccgctggt gaggcatacg gcggtgatga
     1681 gctataatcc gaccgacatc atgtcatacg agaagcttat gttgcctctt ctcaatgacc
     1741 cggtcagggg ggtcaggagt gaggcagcca tcaggctctc cgaggtgcgg gaggagcggc
     1801 tgagcctccc tgcccgtaag gcgcggaggg cggcgctgga tgagtacatg cgtatcaacc
     1861 tgtacaatgc cgattttccg ggaggcctct tcaatcttgg ggtcatgtat gccaatgccg
     1921 gtgacctggc gcgggctgag gcagcataca gggatgccct tggtattgat gatcagtttt
     1981 atctggctaa ggtgaacctg gcaactctct ttgcgcagca ggggaggccc ggcgaggcag
     2041 agaaactgct tcgtgatctg ctcagagatc acccggaact gcatgaactc aacagttcac
     2101 tggcgatgct gctggccgaa caggggagat acggggagag ccgtgagttc ttcctgaaag
     2161 cggccgggct gatgcctgaa cagacgagaa tactctataa tctcggcctg ctcgagaaca
     2221 agctcggtaa tacaatcagc gccgaaaagt atctcacgga ggccctgaac agggaccccg
     2281 gcaattatga ttatctgtat gccctggcca cattttgcct ggagcacggt aaaaaagaga
     2341 gagcgctgac ctatgccagc cggatggcgg cccggtaccc gggtgatcag gccgccaggc
     2401 agctgctcag ggcggcaggc aaaaattagg atgccgctgc cagggtctct tacaattacc
     2461 tgtcaacagg gtatggctta tgctgcggaa atgaacgcgg ctcttccgag atctttttca
     2521 tgacaacctc tacggccttc atcaactgcg tgtcataccc ctccgagtac tttttcgaat
     2581 ctatgtcaat gattatatca ggagtgacac cctcgttctc cactacccat tcacccttct
     2641 cattgtatat gcgatagtcg ggcgctgtga tgcttcctcc gtcaataagc tcaatgaact
     2701 gtgacacacc caccagtccg ccccaggtgc gggttcctat taccggtccc atcccgaacc
     2761 agcggaactc gtaggggagt tcatcgcccc ccgagccggc atacctgttt gttatcaatg
     2821 ccatgtgtgc gtttacggca aggtggggaa tgggctgatc agcagactgc ctgcgggtcc
     2881 agtatccatg aggctttttc tgcagacgct gcaggaagat ttccgggtca agcccgccgc
     2941 cattgaaacg accgtcaata atgagtcctt ccttctttgt ctgcgagtag aagtatctcg
     3001 ggaaatcagt tgctgagccg ttccaggtgt caggcaggta tatgtagcca atctttccgc
     3061 cggaggcttt atccactgtc atacggtttg actcgagcca gtccatataa cggaatctgg
     3121 tttcactatc ggacggaaca actgttacct cgcgtgcacc tgtcagcgtc ggttttgagt
     3181 ttacagtcag cgttacctgc ttgccggcca gacccacgaa atagctgtaa acttccctgt
     3241 cagcctttac ctcattgtta ttaaccttta gcaggtagtc accttcggta atattaacac
     3301 ccggttttgc cagcggtggc caggcctcgc gcgaccagtc cttttccctg aatatcttgc
     3361 tgaagcggta gaagttattc tgtttatcca cagagtaatc agccccgagc atcccggtat
     3421 tgaccggttc ggaacgccgt ttggcatcgc cgccgtaaac atatgtatga gaagtattaa
     3481 gctcggctat catctcaccg atgataaagg tgaggtcctg ccggcatgtt gccctgtcag
     3541 ccagcttttc atatttgagc ttgagagcgg gccagtcctg cccgtgcatc cccggttcat
     3601 aataatagtc gcgctccatg cgccaggcct cattgaatat ctgcttccac tcgcttaccg
     3661 gatcatacca gaccttcatg tcggtcagcc tgagcggcga aggatttcct ccgtctgccg
     3721 gcatcagtga tactccgcca tccttccgga gcacaatggt gcttccgtcg gcagagagcc
     3781 tgtagtcatc aatcccttct gcaatctgag acgcttttcc cgatttaagg gaccatgaga
     3841 acaggttcat cggcccgtgt ccggagacct cgaaccggtt gaagttgccc tcatcactgt
     3901 tgagccagaa gatagacctg tcatttacgg cgaggttgcg gtagttgccc tttgcaaccg
     3961 gaagggcttc aacgcggtct gttattccgt cgaagtctat cttcaccatg agtggtgaag
     4021 ccggagcagc agcaacagct gtttttccgg ctgaacggcc tgcctgacct ttaccggcat
     4081 caacggttcc cgtgctgacc ggagccaccg ttgatgaaac aggttcctca tcactcctcc
     4141 agggcataag tgatttaccg tcttttctga gcgttacagc atagactcct gccaccttct
     4201 ggtaaaccat ctcccactcc aggtcacaat atgtgggact aaaccttcgg ttggagatga
     4261 acaggatata ctctccgtca ttggtaaaaa caggattgaa atcatggaag agcccgttgc
     4321 tgatgccgtt gatgcttttt gtttccagtc cgtaaacata gagctgatac atgtagtctt
     4381 cgttcatttt cgagtagact atatagcggc tgtcaggtga ccatgaataa tcaaagattg
     4441 atttgaggtc aagagaaacg tcaacgttct catactcttc cttgtcaacc tttgtaattg
     4501 cctttgtggc cacgtcaatg taccacagtg tcagggtctg gtctgtccat gcaatttttt
     4561 tgctgtcagg tgaccatttc agtgtatggc ggtatcctgc tcccagggag gtaagcttca
     4621 gcggctcctc cttgccgtca ggtttcatga catagagttc atattcgcct gaggcgtcgg
     4681 agaagaaggc tatccggctg ccgtcgggtg accatactgc atccttgtcg cgggcgccgg
     4741 atgaggctgt caggttacgg accgggccct ccttttcagg aagagtgaat atatcgccac
     4801 gcgcgactac cagtgcacgt ttgccggagg gtgagatatt gacaccggtg atattgcggc
     4861 tgacatcttt catatacggg cgccgctcct ccatgtcggt aagtattttc accggtaccc
     4921 tggcagagac acctgtagtg aggtcaagct tccagatatc tcctcccagt tcatatacaa
     4981 tctggttgcc tccgtagtcg gggtgtctca catcatattc agtgtgtctg gtaacctgct
     5041 caatcttccc gctgccggtg tcatatgacc agatattcaa taccctgtcg cggtctgaac
     5101 tgaagtatat tttattgcct atccacatcg gccacctgtc tgacccttca tagttgctta
     5161 tattggtctc cttgttggtt tcaaggtcat agatatagac ctcctgtgcc cttcctcccc
     5221 tgtaccgttt ccaggtggca ttatcctgcg agtccttgtt gtaggcaatc ttcattccgt
     5281 caggggagaa gcttccgcgt gcagcatcat acatgatcat ctcttcaagg ccggtgccat
     5341 cgggagagac caggtacatc ttcacatatc gcgaggggct gttgcggccg gaggtgaaca
     5401 tgatcttgtt tttggcagca ttccacccca caacttcatc cgctccgggg tgccaggtga
     5461 gccttgtgat attaccaccg tcaatattca tgacgtagac atcggtgttg ccgtcatatt
     5521 cacccgtgaa ggctatcagg ctgccgtcag gtgagatttc cggcatactt tcacggccgt
     5581 cgctgaatgt cagcctgcag gctgtaccgc cctctgcagc gactttccac aggtcaccgc
     5641 cactggcgaa aacgatagtg ttcccctgga tgtcagggta ttgcatgaat gggtcctgtg
     5701 ctcctgccgt aaatacaggc gcaaggagga gtaccatcag tagcccggca tgtttgattg
     5761 ccctgccgga gtggcattgg ttgttcttca tataacttga taattttggt ttgtcattgg
     5821 cctgtgacta ccatccaggg ccttacagac cattcggtga tgagtccgtt cctgacgtag
     5881 ggatcgcggt ctgcaaaatc atgagctgcc tgtggggtat cacccctgaa taccagcacg
     5941 gccccgtcgg acggttcggc caatgctccg gccatgaaga ggcgaccgtc attgcggaac
     6001 tcgtgcacca gaccgaggtg ctcatcgcgg aacggagccc ggcgctcaat gtagttctcc
     6061 acagtcttgt aaaacaggat gtaaaacata cggaagttat ttgtctgtat taatgttata
     6121 ttgttgcaac tgatttcagc cgcataattt tttatgacgt gacaagttat aaatttcaac
     6181 ggatgtcagt aaggccggag tagttaaaac cataccctga caaataaatt cttctcaggg
     6241 aaaggagccc ggataatttc tgaagagtta cttttgtgcc ctgattcagg tacccgatca
     6301 gttcattgct gacaggagct gatcacgggc aattattctg atgagacgaa aaagtacata
     6361 cagcggttcg atacctttaa aaataagtat catcttccgg tcactccgcc accgcaatta
     6421 ccggttgttc ttcagcgggc agagcatctc gctgatagga acctggatgc agaggatagc
     6481 gttgccgtgg gttgtctaca ggatgaccgg ctcagaggtt ttgctgggca ttgtgggttt
     6541 tgccggctca ataccctctt ttctgctggc tcccttcgcc ggagtgctga tagaccgctg
     6601 gagccgctac agggtgttgc ttttgactca gataatctcg atgctccagg caggtgtgct
     6661 tgcatggctc agcctcagcg gaagcctgga aatatggcat atactggtac tgagcgtggc
     6721 actgggctgc atcaactcat ttgacatgcc ggcacgccac tcttttgtaa ttgatatggt
     6781 aaacggcaag gaggatattg gcaatgccat agcccttaac tccatgatgt tcaacggcgc
     6841 caggctgata gggccttccg tggccgggat agtcctcgct tccgtgggcg agggcgcctg
     6901 tttcctcatc aacgcgttga gttacatctt cgtcatcgct tcgctgatga tgatgagagt
     6961 gacggagacc cgcgaaaaga aagagcctgt tccgatgttc cgggagatga aggaggggtg
     7021 ggattacgtt ttcggctttg cgccgataaa gcatattatt ctgctgctgg cactggtcag
     7081 tcttatggga gcctcatacc aggtgctgat gccggtttat gcgaaggaca tactgcaggg
     7141 cgactcacac acttacggat tcctgatggg cggggcgggc gccggagccc ttctcggggc
     7201 catttacctt gcctcgcgaa atacggttct gaggctggga cggctgattc ctgcagctac
     7261 ggcattgctt tcgaccggat taatagccat ctctatcagt aaatcattct tcatatcgat
     7321 gttcctgatc ttctttaccg gtcttgggat gatggcgcac acagccgcca gcaacaccgt
     7381 tctgcagacc atcgccgacg atgacaagag ggggagggtc atgagcttct ataccatggc
     7441 cctgatgggc actgcaccgt tcgggagcct gctggtagga tggatggcca aagtgctcgg
     7501 caccccgtgg accatcttca tcggcggtgc tgcctgcctg ctgggagcac tggtcttcta
     7561 ccggaggctg cccgagctca aacggctggt cagacccgtt tatgtcagga tgggcatcct
     7621 gcccgaagtg gcgaaaggaa tccgcacagc ctctgaaccc ggatcaggag tctgaacccc
     7681 gtttcttaca aaattccgca aatcgggcct ttcgttccgt cggtaaaaaa aacctttccg
     7741 tcggtgagta ttcccttccg gtctaaaaga ttttcagccg gcaccttact acaatagttt
     7801 tgtctcatca tgtttcacaa aatgaaaaga atgagaacga aacgatctat tgcagtggca
     7861 gcggctctga ttattggctg cactgccgtc actgcccagg cgaagcagtg gtcactgacc
     7921 gactgcatcg gatatgctct ggaacagaat attcaggtga ggaaggccgg tgtctctgtc
     7981 agtctgggag aactgaacct tcagcagtca aaggataata tgctgccatc cctcagcgca
     8041 tcactgagtg agaacctggg ctggcgacag gaaacaaacg ccccggatga aacctcatgg
     8101 gcgggttcct cacgcaccaa cgcctcgctc agcagcggac tgacgctctt caatggtttt
     8161 cagctgaaaa accaggtaag gcaggcaggg ctcgatttaa aatcattgca atacagcgct
     8221 gaccagacaa tggaaagtat atcactgaac attatgagcg cttacctcca ggttctttat
     8281 tcagaggaac aggtaacaaa tgccggtaac caggcagagg ttacccgcga ggagcttgcc
     8341 ctggcagcgg agagactctc gctgggtgcc attgccaaca gcgactacct ccaggttaag
     8401 gctcagcttg catcagagga gtcgtcgctg gcatcagctg ccaacagcct taagatggca
     8461 aggctgaacc tgatgcagct gatggaatac cctgtagatg aggcgttcga gattatacgc
     8521 cctgatattg atgccatcat ctccacagca ccagctactg atgcggtgtc actctacgag
     8581 gaggccctca gggtaaagcc ccaggtaaag attgcctcgc taaaccgcga gaacgcctca
     8641 attgacctgg aaattgccag gggaggattc cttccttcac tgtcactgaa tgccggcatg
     8701 agcaccgggt tcaccggggc gacagagatg ggtgcacagc tgaaaagcgg attctcaccc
     8761 tctgtgggat tatcagcttc gataccaatt ttccgtaaca accagaccag ggcttctgtt
     8821 accagggcaa aatacggtat taccacggca gagcttgatg agcttaatac aagaaaccag
     8881 ctccgaaaag aggttgaaca ggctgtcctt gacgccgggt cggctttgat aaattaccag
     8941 gccgccctga aaaggtatga ttcatcagtt gaaaattaca atgtgtcgga ggaaaaattc
     9001 aagctgggag ctatgaactc ggttgatttc ctcatacaga agacaaatct taccgctgcc
     9061 gagagcaacc tgctccaggc gaaatacgag cttgtctaca gccacaagat aatagacttc
     9121 tacaggggag ttgaactgac attctgagat aaaaacagta aacaataaac aatgatgaaa
     9181 aagagcaatg tattcataag cgccgcgctc cttacggtgg ctgttgccac tctggccacg
     9241 gtgcacagtt gcaggaaaag caatacctac cggtatgaga ctgtaaaggt gacgaggggc
     9301 tcgattgtca atgaggtcac tgccaccggg acccttgagg caatcacctc tgtggtggtg
     9361 ggaacacagg tgtcgggtat agttgaaaag ctttatgtcg atttcaactc accggtaaag
     9421 aagggtcaga ttcttgcaga gcttgacaag acagccctga ggtcaagtgt tcagcagtcg
     9481 ctggccagcc ttgaaaactc aaaagcagag atggagtacc aggcatcgaa ctataagcga
     9541 agcaaggctc tccgggagaa aaacctgatt gctgaagctg attatgacca ggcgaagtac
     9601 aactatgaca ggtcagtggc taccctgaag aactcacagg cacagtatga caaagcgctt
     9661 gtacagcttg gctatgccac tatctattcg cccattgacg gggttgtgat gaacagggct
     9721 gttgacgaag gtcagacggt ggcggcaagc ttcaatacgc cggagatttt tacaattgcg
     9781 caggatctca cccagatgca ggttgaggct gacataaacg aatctgacat aggtattgtc
     9841 agggtaggcc agagggttga atttgacgtt gatgcatata ccggagagaa attcaccgga
     9901 acagttgaac agataaggct tgcgcctgtg acaacatcca ctgtggttac ctatacagtt
     9961 attatcaatg cccctaaccc ggatcagaaa ctacttccgg ggatgacagc caacattgtt
    10021 atctatgcgg aagagcatct tgatgttctt acccttcctt acaaggcggt caagttcaaa
    10081 ccggatgctg agtacctggc caggatggct gaagatttcg cggcgggcaa ggcgcccggc
    10141 acgatgaatg ccaaagcagg ctccggagca tttccgggcg cgggctcagg gccactccct
    10201 ggcggaggtt caagaccact cccgggagca ggctcagggg cattcccggg tgcaggttcc
    10261 ggaacattcc cgggcacggg agcgggacca ttccctggta ctggcacagg tgaaggattt
    10321 gagatgcctg ctatggtatg gatcagggcc ggagagggaa tccagcctgt tcccgttaag
    10381 cttggatcaa atgacggtgt aaatgccgag gtgatttccg gcctcgccga aggtgacgag
    10441 gtgattgtga agatgaccgt agccaccgca aaggagaaaa aggaaaaaaa ggcagcctcc
    10501 aatccgttta tgcctggccc tccgggaagg agaaacacgc agggcggttc acgggcagga
    10561 acagattcat ccacaaagag ataacaatga agacaatcat tgacatagcc aaccttaaaa
    10621 aggattatca cgtgggtgag gtcacggttc acgccctcag aggggtggat ctgaagataa
    10681 acgagggtga gtttgttgcc atcatggggg ccagcggttc aggcaaatcg acgatgctga
    10741 acatcattgg ctgccttgac cggccaactg acgggaccta cctgcttgac aacgtcaatg
    10801 ttgcaggaat gtcgaagaat gaacgtgcgg caatccgcaa cctgaagatc gggttcgtct
    10861 tccagtcgta caatcttctc tcacgtacat ctgcactgga gaatgttgaa ctaccgctga
    10921 tgtataatcg ttccgtaagc tccagggagc gcagggaaaa ggccattgcg gcccttgaag
    10981 cggtggggct ggctgacagg atgaatcata tgcccaacca gctctcggga ggtcagcagc
    11041 agagggttgc aatagcacgc tcactggtga atgacccggt tgtaatcctc gccgacgagg
    11101 caacgggaaa ccttgatacc agaacctcat atgaaatcat gtctctcttc caggagctca
    11161 acaataaggg aaagacaata gttttcgtga ctcatgagcc cgatattgca aagtgcatga
    11221 cacggagcgt ggtgttccgc gacggaagca taatcaggga ggaagatgtt aaaaacaggc
    11281 tgaatgcggc agaggtgctc aggactttgc ctgtggaaca ggactaaaaa ggagagaaat
    11341 gaatccggga aatcttttaa aaatagcaat caaagcattg aagcgcaaca agatgcgctc
    11401 attcctgacg atgctcggga taataatcgg cgttgcatcc gtaattacaa tgcttgcaat
    11461 tggccaggga tcaaagaaaa gcatccagga tcagatagca ggcatgggtt caaacatgct
    11521 gtttgtaatg cccggcaata tgaggatggg aggggtacag caggggagct cttcatcaca
    11581 gcggcttacc gtggcagatg taaaagccat caaaactgaa tgtgatgcca ttgttgccgt
    11641 ctcaccggag gtaagaagca gcggtcaggc tgtctccggc aattcaaact ggccaactac
    11701 aatctacgga gggaatgaac agtatcttga gatcaggagc tgggatgtgg tcagtggtcg
    11761 caatatcact gacaccgagg cggaaagttc cgcaaaggta tgccttgtgg gccggacagt
    11821 agccgatgag ctgtttggtg aagggattga ccccacggga gagacaatcc gttttcaaag
    11881 cattccgttc aagatcattg gcgtgctctc gaaaaaaggc cagaacagtt tcgggcagga
    11941 ccaggatgat gtgattatcg ccccgtacac cacggtacag aaaagaatcc ttgcacagac
    12001 gcacatacag tcaatccaga tgtcagcccg cagtgcggag gagtctgaac tggcgcagtc
    12061 tcaggttgag gaggtccttc gccgtaccca taaactgcgc gagaacgagg acaatgactt
    12121 cgaggtccgc agccaggagg agcttgcaac tacaatgact tccgtgactg agatactgac
    12181 catactgctt ggcgccatag ccggtatatc actcctggtc gggggcatag gcatcatgaa
    12241 tatcatgtat gtttcagtca ccgagcgtac gcgggaaata ggcctcagga tgtcggtcgg
    12301 cggcagaagc attgacatac tgcttcagtt cctcattgag tcgatactgc tgagcgtttt
    12361 cggggggctg atcgggatag taatcggttt cggagcttca gccattgttg aagcgctcac
    12421 ctcgtggccc ataagcgtta tgtggagctc ggtaatcctt gccttcgcag tatgcacagt
    12481 gataggtatc ttcttcggct ggtatccggc acggcgggca gccgatcttg acccgataga
    12541 cgcactgaga tttgaatgat taaaccagat tgattagctt tgatgtaggc atgaaaagtg
    12601 attcaatggc taaaaggagg aggggactgc ccgttttgct gcatgcaacg ggatggataa
    12661 tcctgtttat cctgccccag tttctgatct cgggagggtt gttcaatgat gcacgtacaa
    12721 ccagggttgt gctcttcaat acccttgtgt tcctgatact tttctatacc aactatttct
    12781 ggctggttcc ccgccttgcc cagaagaaga agtggcttgc cttcctggtg ctggcagcat
    12841 cactcatcgt tttgctgggc tatctctccg gcaagtatta cgagaatctt ttcgcgccgc
    12901 cgcgcgaagt aagggagcgt atcagggagg tggcagatca caggcctcag ggagataacc
    12961 gcatgccgcg aaggggaggg gggatggtgc tttataactt cttcatcaca tcattccttg
    13021 tctcaggctt tgccgtgggg atcaggtacg ctgagagcgc cgtcaggaaa gaggaggaga
    13081 taaaggagct ggaacgcgag aagctgaatt cggagctggc gctcctcaag aaccaggtca
    13141 gcccgcactt tttattcaac accctgaata atatctattc actgataggg ataaaccggg
    13201 acgatgccag ggatgcggtg ctgagcctct cgaagatgat gagatacatg ctctatgagt
    13261 ctgagcaggg taataccagg ctcagcaatg agatagcatt catgaagggt tacattgacc
    13321 tgatgaagct caggctcagt gaccgggtaa agctgacggt aagtttccct gaggattatg
    13381 aggatcagga tgtgccgccg ctgcttttca ttccgtttat tgaaaatgcc ttcaagcatg
    13441 gggtcagcgc agcaggcaac tccttcattg atattgcact cggggttgaa gaggggatga
    13501 ttaccttcat tgctgccaac agcattggcg gcagagtttc tgacggaccg caggttgctt
    13561 caggcatagg gcttgacaac gtaagaaaga ggcttgccct tctctacccc ggccgtcatg
    13621 atctgagaat tgaccgccag gaatcggtat tcacggttaa actgactata agaagctgat
    13681 agtgtctgtt catgacaagg gttgtagcca tagatgatga gcccctcgca ttgcagctgg
    13741 tgaagggtta tgttgaaaag acgccaaccc tggagctggc cggggcattc gacaatcctg
    13801 ttgatgctgt ggcattcatc cggtcaggcg atgttgacct ggtgttgctt gacatccaga
    13861 tgcctgacct gacgggaacc gagctggcca gggtgattgc cggcggcccg aaagtgattt
    13921 ttaccacggc ttatgagaag tacgcgctgg agggattcag gctcgatgca gttgattatc
    13981 tgctcaagcc gttcagctat gccgagttcc tgaaggccgt tcagaaggcg gagaagctga
    14041 taaaccttga gaggaaggag ctgccttcac tggagatcag gaatgagttt ctctttatca
    14101 ggagcgatta caagatcagg cgcatcgatt tttcagagat acactatatt gagggtctga
    14161 aggattatgt aaagatcttc ctgaggggag agaagaagcc tctcctgtcg caatccaccc
    14221 tgaaggcgct tgaatccagg ctgccggagg ataggttcat gcgcgttcac cgttcatata
    14281 ttgtcaatct tgagactgtt aaggtcatcg accgtggacg tattgtctat ggcgaggtgc
    14341 ggatacctgt caccgaacag taccgtgaca ggttccagga gtttcttgac cggaacttca
    14401 tctgagaaca aaaatcactg agccggggaa tcaatcaggc agacaatctc atcaaacccc
    14461 ggagccaatt tcttttttct cttcataatt taaagatcgg aattataata tattgaatag
    14521 aggcctcttg cattataaac atcaagagtt acactgcgtc tgcttaatga ttaacaacaa
    14581 tatgatttgt gtcatttatt agtgcaaacc gcatgttatc tttgtcggcg atctgaaaaa
    14641 aaatgcataa ctaaaaaaga aagaaaaaat gaaaacaagg tttgctctgt taatgattct
    14701 ggcactggtt gctgtaatgt tttcaggctg cgagaaggaa aaggaagatc catctgtaac
    14761 cctcggtgcg caggctaatg caacagtacc cggattctat tcggtttcac ttgataagac
    14821 atacaccatg agccaggctg ctgccagccc cggggtaata gacatattat gcttctatga
    14881 ggctgagggt ggcaacaaca tttctcttgc atcgccgggt tcaggcatta aggaaatttt
    14941 caccggtgat gacaggcccg atacctggag cataaagaat accacttttt tcttccagac
    15001 cgcactgaca gcatcccagt ttgaggctgt acaggatggt gatgcactta ttgaaacctc
    15061 ctttgatgct gacaacggcc gcaaaaaggc caaggatgta caggtaggac aggtctgggc
    15121 gttcaaaact caggacggca cctacggact cctgcttgct actgcagtaa cccagggcac
    15181 agacggtact gttaccttca agctccggac aaagtagttc tgccatggca aaacggattc
    15241 agtgcatagc gttactgatc atcattgttt ttgccggttg cgtcagggag gaccctgaga
    15301 ccacgcccct gatcctgctt aagacaggcg cagcttttac cgctgatggc tcggaagtgc
    15361 cgcccggggg agtactcagg ttcggtctct cggtatccgg aggaggcggg gccatcacaa
    15421 acctggtggt taaaaggatc tctgacggtg tggctgtcac tgaagctgac aaaggcatgt
    15481 atatcagtta cgggggactt gacaccaccc tgatctatac caaaagctat gctcagactg
    15541 agcggtgggt gttttcagtc atgaactcgt accgtgacac ggcatcagta tcactcacgg
    15601 tactgaaagg cgccggctct gcctggggcg agattaacta ccacccgtcg ataaaggcag
    15661 gcctgcagga taatcagaca ttgccccatt tcgtggacct tcattcagga gctgtctggg
    15721 atgctgaaaa cgtgacgggt cacgaggcag aggttgacat ggcagccttc tggtatatta
    15781 cctccggcac ctcatcaccc acactcacct gtccggcata ttcctccgca cttacatact
    15841 atcccctgtt cgggtcatgg gatgtaagga accagacaat gtatgactac tacacttctg
    15901 acaatgacc
//
LOCUS       DAOPBK010000044        15543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3871_length_15543_cov_4.898631, whole genome shotgun sequence.
ACCESSION   DAOPBK010000044 DAOPBK010000000
VERSION     DAOPBK010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_15543_cov_4.898631"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLA17_04080"
     CDS             <1..933
                     /locus_tag="PLA17_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPK84624.1"
                     /translation="GIKPEEVDSILSIPFMPISFFRDHLVMTGGGEAERIFLSSGTTA
                     IRGDTGDMKNQADTEVRNQSGAVNPKRSRHEVKSLTLYNESLERTFRMFYGDPGGYAI
                     TGLLPSYLEREDSSLIYMVNRLMQLSGNDAGGFFPDDHTALLQTIEKARAARLKVLLM
                     GVTFALLDLAEKHPVDLSDVIIMETGGMKGRRREMIREEVHEIIKKAFGVSSVHSEYG
                     MTELLSQAYSRGDGIFRTPPWMKVLIRDSHDPMSHSSDAGAAGGISVIDLANLWSCSF
                     LATDDLGRMHPGGLFEVQGRFDEADIRGCNLLVS"
     gene            complement(1163..2365)
                     /locus_tag="PLA17_04085"
     CDS             complement(1163..2365)
                     /locus_tag="PLA17_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPK84625.1"
                     /translation="MKKTVLILMAIAVAAMPMYAQKSKKQAAPEPEGYKFTTVVSLPV
                     TPVKNQSATGTCWCFATTSFMESELLRMGKGEYDLAEMYVVRKTYENRLFDNYLRRGK
                     GNLGQGSLSPSWLRVFRENGIVPQEVYSGINYDSQLHNHSELQSYIDAIATVPVKMRR
                     LSEESNEVANAVLDIYMGEVPATFTYKGQSYTPKTFAASLGLNMDDYVHITSFSHFPF
                     YTEGLLEVPDNWEMNRFHNVPLDELIQIMDYALNNGYTVAWDGDVSERGFSHANGVAI
                     NPDLARVESLAGTDRARFEAAQGAPAANQALFEKPGPEINVTQEIRQNGYETYVTSDD
                     HLMHVTGIVKDQNGTKYYITKNSWGTDRNPHGGYLNMSESYVRAKTVFILVHKDAVPE
                     DIRSKMGI"
     gene            complement(2690..3052)
                     /locus_tag="PLA17_04090"
     CDS             complement(2690..3052)
                     /locus_tag="PLA17_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPK84626.1"
                     /translation="MSDRKGLGPIGEELAASWLEGKGYSIRHRNWHSGRTEIDIIAEN
                     STFIVFAEVKTRFEGYQVHPSEAVNVPKQRRIIFAASNYIDTYGITKEARFDIISVII
                     GENGHEIDQIEGAYYPTM"
     gene            complement(3119..3760)
                     /locus_tag="PLA17_04095"
     CDS             complement(3119..3760)
                     /locus_tag="PLA17_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPK84627.1"
                     /translation="MEKIRIALADDHQLFRNGLKILLGGYPEFEVVAEASNGTELLKV
                     LENCPTDIVLMDINMPEMDGVEATSRISRQIPEVFVIALSMYGEEEYYYRMVEAGAKG
                     FILKDSDISDVHDAIIAVHRGGNYFSQELLYHVIRRIKNREQEEKSSNLSRREKEILF
                     KICEGLSNHEIAEELFISKRTVDKHRANLLAKTGSKNTASLILYAIKNRLIEV"
     gene            complement(3979..4926)
                     /locus_tag="PLA17_04100"
     CDS             complement(3979..4926)
                     /locus_tag="PLA17_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPK84628.1"
                     /translation="MVRLVALFISVVLQIVAASIAIRFMKITRYRLSWILLSTSFVLM
                     AVRTLIQLIEYFQGKPSFELMMVDEWMNVLISVMIITGVVLIREIFYSLKRAEIDRAR
                     YDRRLLNAVINTEETEKKRFAKDLHDGLGPLLSTVKMSLSALNSRVTDPVGQEILQNT
                     NHIVNEALNAIKEISNNMSPHVLTNLGVASAISAFAAKVNMMRGVVIDFRTNMEGERF
                     DTDKEVVIYRAACELINNGMKHSGASRIEIDLHRHKKIITLQYLDNGRGFDMEKLSSE
                     EAVGMGIPNIETRVRSVDGVFVIESSRGKGTSALIKVDQ"
     gene            4991..6247
                     /locus_tag="PLA17_04105"
     CDS             4991..6247
                     /locus_tag="PLA17_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPK84629.1"
                     /translation="MKTFYTGLIALYSVMARAAIPFSAKARRWVRGRRGWRERLSSFS
                     RGEGKVAWVHCASLGEFEQGRPVIEKIRRERPDWKMVVTFFSPSGYEVRKDYTGADMV
                     MYLPTDLPANVRFFLDHLRPDLVLFVKYEFWYNYLGELKRRNILTCLVSGIFRPDQYF
                     FRWYGRPARGMFSVFNRMFLQDEQSGRLLESIGYHNYSVTGDTRFDRVSQIAAAAKEL
                     PVIEKFRGGESLFVAGSSWDEDEDIIARYINANSGTMKWIIAPHEIDESHLCRIEKRL
                     APKSVRYSRYDDEAGDCRVMIIDNIGMLSSVYRYASIAAVGGGFGRGIHNILEPACWG
                     IPVLFGPNHLKFREAVQLKERGGAVSFNNFETFATIVEKYLSDPAAIEAAGNASAAYI
                     AENKGSTDKVYKEIFEGQTAENLLKK"
     gene            6404..9676
                     /locus_tag="PLA17_04110"
     CDS             6404..9676
                     /locus_tag="PLA17_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPK84630.1"
                     /translation="MRKLKITLMLLSLIIGSATLMGQVTTSSLTGTISDENGDPMAGA
                     TIVATHVPSGTVYGAIANSVGLYSIQGMRPGGPYTVNVTFIGYKTQSYTEITLLLGEN
                     TIMNSTMVPTTTEMTEITVVGYATSKFSTTKTGAVTNISNTQIANLPTVSRSIMDVTR
                     LSPYGGNGMSFVGSDGRTANFTVDGANFNNNFGLSSALPGGGSPISLESIEELQVVIA
                     PYDVRQTNFIGGGVNAITKSGTNTFKGSLYTFHRNENMRGDAVYGNPIAGARNIDRNT
                     TYGFTFGGPLIKNKLFLFANGEMAKTPTVVNRWRASTDGVADPDNYISRTTINDLRTV
                     SDYVKSKYGYDTGSFTDFPADENNYKLLVRLDWNMTDKHRVALRYNYTKNLYWSSPNA
                     SSMDGGTRMSEARMSQASMSYANSMYSMNNLVHSFSLDLNSRLSDNLSNQFLATFSKL
                     DDVRGSTSNEFPFIDILKDGQAYLSLGYELFTWNNAVHNNVWNIKDELTYYSGNHKII
                     GGIAYEYKMADNAYMRNGTGYYRYRSLDDFLNEGTPEIVNLTYGYDGESNPAARIRTN
                     KIGIYGQDDWSVTPNFKLSYGLRIDGLFFNNDDLMTNKAIYDLNYSDRHIDTGKWPTA
                     NLIVSPRVGFVWDAYGDKSLKLRGGTGLFAGNLPLVFFTNMPTNGGMVQYQAQINAAN
                     AAKRGFSMDEFAGGLVTDASGQATTAALYNRLISLGYPSTISPEDGTVPSAVNGVDPN
                     FKMPQVWKTSLAVDYAFKTSFPLSVTLEGIFNKTINGVSISDWSIPDVGGFARFNGVD
                     NRPVYPAGYRTKTKAFVLENTKLGYGWSGNITINAQPAEWLNVMAAYAHTVAKDVTGM
                     PGSAAESAFTYVPTVEGPNFIRLHNSQYNTPDRLVASLTAHDNSGNHFSLIYESWLGG
                     ANYSFMMLNDINSDGYNYDVIYVPTDAEVQSNQFRFVSADDKTRFMDYVHANSYLKNR
                     QGKYAEPYSVYSPWVHRIDFGYKHDFVFNANNTKHTLQLSLDLKNVLNLFNSEWGVAK
                     YLNPAIGSEARILKFEGVDAEGVATFSTPSAINGDTKTFTPSHSLGQCWYALIGVKYI
                     FN"
     gene            9693..11189
                     /locus_tag="PLA17_04115"
     CDS             9693..11189
                     /locus_tag="PLA17_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HPK84631.1"
                     /translation="MKLKYFFPLIIAAVAFMASCDDEFSVTLLDEIQVSSSYVSIPVE
                     GGSSSITVTATESWTVVNNLSWLTISSASGSAGESTLSFSASATLDGRTGEVKLQCGD
                     KTQRIIVIQGVPVVATATCAEVIAGPNDKTYKVTGVCTAITNTTYGNWYLQDATGTIY
                     IYGTLDAKGGTKNFLSWGLEVGDEVTVQGPKTTYGTTVELVDVTVLKINKSLVKVDSV
                     ENATLPIEGGEFIAYLTCKGQGVSVEIPEDAESWLSISSIQSEGTSVVVKFKAAPNEG
                     GDRSTTITFYTTDGTKDYSSQTKLSQTGAIIKASIAEFLAAPVGDVQYRLAGVITNIA
                     NPTRGNLTLRDFSGEVFVYGIQDFSTKDLKVGDIITIVGKRAEYNGNPQVGSSVLESS
                     IPVTPATIAEVLAKPDDPNTYFMVTGEIISIASTEWGNLTLKDGDSEIYSYGCYPGYG
                     ATGDARKNLLVDKGIKVGDQLTVIAPKTTYQGTPQLSRSIYFSHTSAK"
     gene            11731..14277
                     /locus_tag="PLA17_04120"
     CDS             11731..14277
                     /locus_tag="PLA17_04120"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPK84632.1"
                     /translation="MGKPSKTQGETSKKTFTYDEALEATRQYFGGDDLAAKVWTSKYA
                     LKDSFGNLYEKTPDDMHRRLASEVHRIEMKYPNPVSFEAIYEAFKDFRYILPQGSPMT
                     GIGNDFQIASLSNCFVIGNKGDSDSYGGIMKIDQEQVQLMKRRGGVGHDLSHIRPKGS
                     PVLNSALTSTGVVPFMERYSNSTREVAQDGRRGALMLSISIKHPDAGSFIDAKLEAGK
                     VTGANVSVKITDEFMQAVIDGKSFRQQYPINSDDPKYVKDIDARKLWNKIVHNAWKSA
                     EPGVLFWDTIKKESVPDCYADLGYETVSTNPCGEIPLCPYDSCRLIAINLFSYVKDPF
                     TPKASFDYDLFRRHVHLSQRIMDDIIDLEQEKIDAIIEKIKADPEDDELKRVEQKMWE
                     NIRHKTGEGRRTGIGITAEGDMLAALGLRYGSDEATEFSVNVHRTLAIEAYRSSTKMA
                     TERGAFPIYNAEREKNNPFINRMRKADPEMVAEMEKSGRRNIALLTIAPTGSTSLLSQ
                     TTSGIEPVFMPFYKRRRKVNPSDKDVKVTFIDEVGDHWEEFTVFHHKFIDWMKVNGYN
                     YDEVKDMSEARINDIVAASPYYKATANDVDWVAKVRMQGAIQKWVDHSISVTINLPSG
                     VKEDLVSELYLTAWKEGCKGATVYRDGSRAGVLVSNSREEKKSNWSPLRPKVLEADVI
                     RFQNNEEKWIAFVGIKDHVPYEIFTGLQDDEMFPIPKSIIKGKIIKNKDDAGNTRYDF
                     QYTDKYGYKNTLGGLSHMFKPEFWNYARLISGVLRYGMPVSDVVDLVSSMKLDSESIN
                     TWKNGVERSLKRYIPNGTKAKGKCENCGSTSLVYQEGCLICTDCGTSKCN"
     gene            complement(14378..15283)
                     /locus_tag="PLA17_04125"
     CDS             complement(14378..15283)
                     /locus_tag="PLA17_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPK84633.1"
                     /translation="MLGNHFHKLILLVTLAALTVVISCRRIQDPPLPDDSNPFAACVI
                     PGTDNTLDIVSFNVQGFPKEGYLSITTLADLLEAMDPDVVALQEVASETEFNRLVELM
                     PGWSGVFYPINNAEWNLAYLIRVSELETVAGSLRTLFEGDRYAFPRPPMEIMVRHRKS
                     GREIILINLHLKCCEGEDNENRRRSASEKLKEYLDQQRPDDAVVMLGDYNDEIASGSA
                     TVNPFLNFINDDSSYRFADMEIALGSKLWWSYPSWPSHIDHILVTDELFESIDTTMVI
                     KASPCYPDYEVVISDHRPLMIVLTP"
BASE COUNT         4096 a         3656 c         3805 g         3986 t
ORIGIN      
        1 ggaatcaaac cggaagaggt tgattcaatc ctttcaatcc ccttcatgcc aatatcgttc
       61 ttcagggatc atctggtcat gaccggagga ggggaagctg aacggatttt tctgagcagc
      121 ggcacgacgg cgattcgtgg cgacaccgga gacatgaaga atcaggcaga cacagaagtg
      181 aggaatcaat ccggtgctgt caaccccaaa cgcagccgcc atgaggtaaa aagccttacc
      241 ctttacaatg aaagcctgga aagaacattc aggatgtttt acggtgatcc gggcgggtat
      301 gcaatcaccg ggcttttacc atcatatctt gagagggagg attcgtcgct catatacatg
      361 gttaacagac ttatgcagct ctcgggcaat gatgccggcg gttttttccc ggatgaccat
      421 acggctcttc tgcagaccat tgaaaaggcc agggcagcac gactgaaagt gctgctgatg
      481 ggagtcacct ttgcgttgct tgacctggcg gagaagcatc cggttgatct cagcgatgtc
      541 atcattatgg aaaccggcgg catgaagggc cggaggcgtg agatgatcag ggaagaggta
      601 catgagataa tcaagaaagc ctttggtgtc agctcggtac actcggaata cgggatgacc
      661 gaactgctca gtcaggctta ttcccggggg gatgggatat tccgtacccc gccatggatg
      721 aaggtgctga tacgtgacag ccatgatccg atgtcacact cttcagatgc cggagcggcc
      781 ggaggtataa gcgtcattga ccttgccaac ctctggtcat gttcattcct ggcaactgac
      841 gatcttggcc ggatgcaccc tggcggactc tttgaggttc agggcaggtt tgacgaggct
      901 gatatcaggg gatgcaattt actggtgagt tgaagtattg ggtcaggtga gttgaaacga
      961 tggatcaggt gagttgagga agtgcatcag gaggattgct ctccggcatt gtccgtttcc
     1021 ttttaaggtt gacaatctgg acttaatcct caatccttga aaaattacca gctgtttctg
     1081 accacattac cataaaaaaa gtgctcatga attgatcatg agcactgttt ctaaaaaaag
     1141 gttcctcagg tttttccggg gctcagattc ccattttgga cctgatatct tccgggacag
     1201 cgtccttatg aacaaggata aagaccgtct tggccctcac gtagctctca gacatgttca
     1261 ggtaaccgcc atgaggattc ctgtcagtgc cccatgagtt cttcgtgatg taatacttcg
     1321 tgccgttctg gtcttttaca ataccggtga cgtgcatcag gtggtcatcg gaggttacgt
     1381 atgtttcgta tccgttctga cggatttcct gggtgacatt tatttcagga cctggttttt
     1441 caaacaatgc ctggttggcg gcaggggcac cctgagctgc ctcgaaacgg gccctgtctg
     1501 tgccggctaa cgattccacc cttgcgaggt cagggttgat ggcaacgcca ttggcatgag
     1561 agaagcccct ctcgcttaca tcgccgtccc aggcaacagt ataaccgttg ttaagtgcgt
     1621 agtccattat ctggatcagt tcgtcgaggg gcacattgtg aaaccggttc atctcccagt
     1681 tgtcaggcac ctcaagaagt ccttcggtat agaaggggaa gtgcgaaaag gaggtgatgt
     1741 gaacataatc atccatgttc agtcccagac tggctgcaaa tgtcttcgga gtgtagctct
     1801 ggcccttata ggtaaaggtg gcaggcactt ctcccatgta aatgtcgaga acagcgtttg
     1861 ccacttcgtt cgactcctcg ctgaggcgcc tcatttttac cggaacagtc gcaatggcgt
     1921 ctatgtaaga ctgtaattca ctgtggttat gaagctgtga atcatagttg atgccgctgt
     1981 aaacctcctg cgggacaatc ccgttttctc tgaaaacgcg aagccatgaa ggagacaggc
     2041 tgccctgacc caggtttcct tttccccggc ggagataatt gtcaaataac cggttctcgt
     2101 aagttttcct taccacgtac atctccgcga gatcatattc gcccttgccc atcctcagga
     2161 gctccgactc catgaatgaa gtggttgcaa agcaccagca tgtgcctgtg gctgactggt
     2221 tcttcaccgg agtgacggga agggagacca ctgtggtaaa tttatatccc tcaggctcgg
     2281 gagcggcctg tttcttgctc ttctgggcgt acatcggcat ggctgcaaca gctattgcca
     2341 tgagaatcaa aactgtcttt ttcatcatag tattatttag aatttaaagg tatgatggaa
     2401 cccgcatgac tgaaaataat atacagttgt ttgtctgttt gatacatgtg ggtttcatct
     2461 atgtctgttt tggtttgtta atttcagtaa tccggtatat ttttcaaatg aatgctcata
     2521 actacctgac tatcaggatt gatcatcttt ttaaatgaac acgccaatta attgattatg
     2581 tgactgttaa ataaaaactt gccggcccgt tacatttggg acaaatcaaa aatatacaat
     2641 aatattcaaa agggaaatgt tttagagcag cagcgccgtg tggaggaaat cacatggtgg
     2701 gatagtaggc gccctcaatc tggtcaatct catgaccatt ctctccgatt attacagata
     2761 tgatatcaaa cctggcctct ttggtgatgc cgtaagtatc tatgtagttg gaagctgcga
     2821 agatgattct cctctgtttg ggcacgttta cagcttcgga agggtgaacc tgataacctt
     2881 caaatcttgt ttttacttcg gcgaatacga tgaaggtgct gttttcggct ataatgtcta
     2941 tttcagtgcg tcccgaatgc cagttccggt ggcggattga atagcccttc ccttccagcc
     3001 acgaagcggc tagctcttca ccaatgggtc ccagtccttt gcggtcactc atatcatact
     3061 tgccctggcg ggggaggaga gcttattcag gctgctcacg gattgcttta cggagaggtc
     3121 agacttctat cagcctgttc tttattgcgt agaggatcag gctggcggtg ttcttggaac
     3181 cggttttggc cagcaggttg gcccggtgct tgtctaccgt ccgcttgctg atgaacagtt
     3241 cctcggcaat ctcgtggttg gagagtcctt cgcagatctt gaaaagtatc tccttctcgc
     3301 gtctcgacag gtttgaactc ttctcctcct gttccctgtt cttgatacgc ctgatgacat
     3361 gatatagcag ttcctgcgag aagtagttgc ctccgcggtg taccgcgata attgcatcat
     3421 gcacatcaga gatgtcggaa tccttcagaa tgaatccctt cgctccggcc tcaaccattc
     3481 tgtaataata ctcctcctca ccgtacattg acagtgcaat aacgaatacc tccgggatct
     3541 ggcgggaaat gcgcgatgtg gcttccaccc cgtccatctc aggcatgttg atatccataa
     3601 ggacaatatc agtgggacag ttttcgagca ccttcagcag ttcggtgccg tttgatgcct
     3661 cagctaccac ctcgaattcg ggatagccgc caagtaggat tttcagtccg ttccggaaga
     3721 gctggtggtc atcagcaaga gctattctga tcttttccat acaatgctta atgccctgcg
     3781 ggcatgttat tttttacaat gctttcctcc ctcggggata ttacctgtgt cattcttctg
     3841 tcatgtctta ccccttcagg gaatattatc tatgctattt ctctacactg tttcgcccct
     3901 tcgggggaat attatctata ttattcttct gcaatgctta atcccgggga caaatcctgc
     3961 tactgccaac ttccggtttc actggtccac ctttatcagc gcgctggttc ccttgcctcg
     4021 cgagctttca atgacaaaga ccccgtcgac agatctcacg cgggtttcta tgttcggaat
     4081 acccattccc accgcctctt ccgatgacag cttttccatg tcaaatccgc ggccgttgtc
     4141 aagatactgc aaggtaatta tttttttgtg gcggtgaaga tctatttcta ttctggaagc
     4201 gccggagtgc ttcatgccgt tgttgataag ctcgcatgcc gcacggtaaa taaccacctc
     4261 cttatcagtg tcaaaccgct ccccctccat gtttgtccgg aagtctatca ctacgccccg
     4321 catcatgttc acctttgccg cgaaggcgct gatggcgctt gccaccccca ggttagtcag
     4381 cacatgagga ctcatgttgt tggatatctc cttgatggca ttcagggctt cattcacaat
     4441 atggttcgtg ttctgaagaa tctcctgtcc caccggatcg gtgaccctgc tgttgagtgc
     4501 tgagagagac atttttacgg tagagagcag cggacccagt ccgtcgtgca aatcctttgc
     4561 gaaccgcttt ttttcagtct cttccgtatt gattacagca ttaaggagcc ttctgtcata
     4621 tcttgccctg tcaatttccg cacgtttcag tgaataaaag atctccctga taagtaccac
     4681 acccgtaatt atcatcaccg agatcagcac gttcatccac tcatcaacca tcatcagttc
     4741 aaacgagggt ttgccctgga aatactcaat gagctggatg agagtgcgga ctgccatcag
     4801 cacgaatgat gtggaaagaa gaatccatga gaggcggtaa cgtgttatct tcatgaatct
     4861 tatggctatg cttgcagcaa ctatctgcaa cacaaccgat atgaacaggg ctaccaggcg
     4921 taccattgca acaaaaattt aatatataca aaagtatgag tttattacct ttggggcgtc
     4981 aaaaccagga atgaaaactt tctatacagg cctcatcgct ctttactctg tcatggcacg
     5041 ggcagcgata cctttcagcg ccaaagcccg cagatgggtg agaggcaggc gtggatggag
     5101 ggaacggctc agttcattca gcaggggaga agggaaagtg gcatgggttc actgtgcatc
     5161 actcggtgag tttgaacagg gcaggcctgt tattgaaaag ataaggaggg agcgtccgga
     5221 ctggaagatg gttgtgacct ttttctctcc atcggggtac gaagtgagaa aggattacac
     5281 aggagctgac atggtcatgt accttccgac tgatctgcct gccaatgtca ggttttttct
     5341 tgatcacctg agacctgacc ttgtgctgtt tgtcaagtat gagttctggt acaattacct
     5401 tggtgagctc aagaggagaa atatcctgac ctgtctcgtc tccggaatat tcaggcctga
     5461 ccagtatttc ttcaggtggt acggcagacc ggcaagagga atgttttcgg tgttcaaccg
     5521 catgttcctc caggatgagc agtcgggccg gcttcttgaa agcataggat atcataatta
     5581 cagtgtgacc ggtgacaccc gtttcgacag ggtcagccag attgccgctg ccgcgaagga
     5641 gcttccggta attgaaaaat tccggggcgg agagtcactt ttcgttgcag gctcaagctg
     5701 ggatgaagat gaagatataa ttgccaggta cataaatgca aacagcggca caatgaagtg
     5761 gatcatcgct ccgcatgaga tagacgagag tcacctgtgc cgtatagaga agaggcttgc
     5821 ccccaaaagc gtccgctatt cgcgatacga cgatgaggcc ggagattgcc gggtaatgat
     5881 tattgacaac atcggcatgc tctcatcggt ctaccggtat gcatccattg ccgccgtggg
     5941 cggagggttc ggaaggggga tacacaacat tctcgagcct gcatgctggg gcataccggt
     6001 gctttttggc ccgaaccacc tgaagttccg tgaggcagtg cagctgaagg agcggggcgg
     6061 ggccgtctca ttcaataatt ttgagacatt tgcaacaatt gttgaaaagt atctctcgga
     6121 tccggcggcg attgaggcag ccgggaatgc cagtgctgca tatatagctg aaaacaaggg
     6181 ttctacagat aaagtatata aagaaatttt tgaaggccag accgctgaaa acttgttgaa
     6241 aaagtgataa aaagttgttt ataattccca aatgccattt ctgacttatt atgctcataa
     6301 ctgtaattta acccttcacc caatgagaac cgaaacttga cccgaaccaa ttgaaattca
     6361 atacatttaa cagaatacaa caacaaccat aacttattca actatgcgaa aactaaaaat
     6421 tacactcatg ctgttgtccc tgattatagg gtcagccacc cttatgggtc aggtgacaac
     6481 ctcgtctctc accggtacaa tatccgatga gaatggtgat ccgatggcag gagctactat
     6541 tgtagccacc cacgtgccat caggtaccgt ctatggtgca attgccaaca gtgtgggtct
     6601 ctattcgatc cagggcatgc gcccgggcgg cccttatacg gtcaatgtaa ctttcatcgg
     6661 ctacaaaacc cagagttaca cagagattac cctgttgctc ggggaaaaca caatcatgaa
     6721 ctcaaccatg gtaccaacca ccaccgagat gacggagatt accgtggttg gttacgccac
     6781 atcaaaattc agtaccacga aaacaggtgc ggtgacgaat atctcaaata cacagatagc
     6841 taatctgcca acagttagcc gcagtattat ggatgttacc cgcctttcac cctacggagg
     6901 gaatggtatg agctttgtcg gctctgatgg ccgtaccgca aactttacag tggatggtgc
     6961 gaatttcaac aacaacttcg gtttgagttc tgccctgccc ggtggaggca gcccgatctc
     7021 tcttgagtct atcgaagaat tgcaggttgt aattgctcca tatgatgtca ggcagactaa
     7081 ctttattggt ggtggtgtaa acgctattac caaatcaggt acaaatacct ttaaaggcag
     7141 tctctatact tttcacagga atgaaaacat gcgcggtgat gctgtctatg gtaacccaat
     7201 agcaggtgcg cgcaatattg accgcaatac aacctatggt tttacttttg gcgggccact
     7261 tatcaaaaac aaactgttct tgtttgcaaa cggtgaaatg gctaaaacac caaccgttgt
     7321 caaccgttgg agagcttcaa cggacggtgt tgccgatccc gacaactata tttcccgcac
     7381 aacaataaac gatctcagaa cggtttctga ttatgttaaa agcaagtatg gttatgatac
     7441 gggatcattt accgacttcc ctgctgatga aaacaactac aagcttcttg tgcgtcttga
     7501 ctggaatatg acagacaagc atcgtgtggc cctgcgttat aattacacga aaaaccttta
     7561 ctggagctct ccgaatgctt catcaatgga cggaggcacc cgtatgtctg aggcaagaat
     7621 gtcacaggcc tcaatgtcgt atgcaaactc gatgtattca atgaacaatc ttgttcattc
     7681 attctccctt gacctgaaca gccggttgtc agataactta tcaaaccagt tcctggcaac
     7741 attctcaaaa cttgacgatg tccgcggatc tacttcaaat gaattcccct tcattgatat
     7801 tctgaaagac ggacaagcct atctctctct gggatatgaa ctctttacat ggaacaatgc
     7861 tgttcacaac aacgtctgga atatcaagga cgagctgaca tactattcag gcaatcataa
     7921 aattatcgga ggaatagctt acgaatacaa gatggcagat aatgcctata tgcgtaacgg
     7981 tacaggatac tatcgctaca ggagtcttga cgacttcctg aacgaaggaa cacctgaaat
     8041 tgtaaacctg acttatggtt atgatggaga gagcaatcct gcagcccgca taagaacaaa
     8101 caaaattgga atttacgggc aggatgactg gagcgttacc ccgaatttca aactttcgta
     8161 cggacttcgt atcgacggac ttttcttcaa taatgatgat ttgatgacaa acaaggccat
     8221 ttatgacctg aattattccg atcgtcatat tgatacaggg aaatggccca cagctaatct
     8281 cattgtttct ccccgcgtag gttttgtgtg ggatgcatat ggcgacaaga gcctgaagct
     8341 tcgtggaggc accggtcttt tcgcgggtaa tttgccgctt gtattcttta ccaatatgcc
     8401 taccaacggt ggaatggtac agtatcaggc acagataaat gcagcgaacg cagcaaaaag
     8461 aggtttctca atggatgaat ttgccggcgg tcttgtaact gatgcaagcg gacaagctac
     8521 tactgccgca ctttataaca ggttaatatc attgggatat ccttcaacta tttctcctga
     8581 agatgggaca gttccctctg ctgtcaatgg tgtggatcca aatttcaaaa tgccccaggt
     8641 atggaaaaca tcacttgccg ttgattatgc attcaagaca tccttcccgc tctctgtaac
     8701 gctggaagga atcttcaaca aaaccatcaa cggtgtttcc atttctgact ggagtattcc
     8761 agatgttggt ggtttcgccc gtttcaacgg cgttgacaat cgtccggttt atcctgccgg
     8821 ttatcgtact aaaacaaaag cctttgtgct ggaaaacacg aaactcggtt atggctggtc
     8881 aggtaacatt actatcaatg ctcagccggc agaatggctt aatgtgatgg ctgcctatgc
     8941 acacacggta gccaaagatg ttaccgggat gcccggatca gctgcagaat ctgcatttac
     9001 atacgtaccc accgtggaag gccccaactt catcagattg cacaattcac agtacaacac
     9061 acctgatcgt ctggtggctt cgctcaccgc acatgacaac agcggcaatc acttcagcct
     9121 tatctatgaa tcatggcttg gtggagcaaa ctactcattc atgatgctta acgacatcaa
     9181 cagtgacggt tacaactacg atgtaatcta tgttcccacc gacgctgagg tgcagagcaa
     9241 tcaattcagg tttgtttcgg cagatgacaa aacccgcttt atggactatg ttcatgccaa
     9301 tagttatctg aaaaatcgtc aggggaaata tgcagagccc tacagcgtct actctccctg
     9361 ggttcatcgc attgactttg gatacaaaca tgattttgtc tttaatgcaa acaataccaa
     9421 acatacgctg cagcttagcc ttgatctcaa aaacgttctg aacctgttca actctgagtg
     9481 gggcgtcgcc aaatatctga atccggcaat cggttcggaa gctcgtatcc tcaaatttga
     9541 aggtgtggat gctgaaggtg tggctacatt ctcaacaccc tctgctatca atggtgatac
     9601 caaaacattc acaccaagcc actcattggg tcagtgctgg tatgctttga tcggtgtcaa
     9661 atatattttc aactaattaa agtacaggca atatgaaatt gaaatacttt tttccattaa
     9721 tcatcgcggc agttgccttt atggcaagct gtgacgatga attctctgtg acgttgcttg
     9781 atgaaatcca ggtatcgtcg tcttatgtgt ctatcccggt agagggtggc tcttcatcca
     9841 ttaccgtaac agctactgag agctggacag tagtgaacaa tttatcatgg ctgactatct
     9901 cttcagcctc cggcagtgca ggtgaatcaa cactttcgtt ttcagcttca gctaccctgg
     9961 atggccgtac cggagaggtg aaacttcaat gtggagacaa aacccagaga ataatcgtta
    10021 tccagggcgt gccagtagtt gcaactgcca cctgtgcgga agtaattgcc ggaccaaacg
    10081 acaagaccta taaggtaacc ggggtttgta cggcaataac caacaccacc tacggcaact
    10141 ggtatttaca ggatgcaaca gggactattt atatctatgg tactcttgat gcgaagggtg
    10201 gtacgaagaa ctttctaagc tggggacttg aagtgggtga tgaagtaact gttcagggac
    10261 caaaaacaac ctacggcaca acggtggaac tggtagatgt aacagttctc aaaatcaaca
    10321 aatcacttgt gaaggttgat tcggtggaaa acgcaacgct tccgatcgaa ggtggtgagt
    10381 ttattgcata ccttacctgc aaaggacaag gcgtatcggt agaaattcct gaagatgccg
    10441 agtcatggtt gtcaatttcc tccatccagt cggaaggaac ctctgttgtt gtgaaattca
    10501 aagccgctcc caatgaagga ggagaccgct caaccacaat cacattctat accactgacg
    10561 gtacaaaaga ctactcttca caaacaaaac tttcccagac aggagcgatt ataaaggcct
    10621 ctattgccga gtttcttgcg gctccagttg gtgatgttca gtaccgcctg gcaggtgtga
    10681 taaccaatat tgccaatccc acacgtggca atttaaccct gagagatttc tcaggcgagg
    10741 tgtttgtgta tggtatacag gatttcagta ccaaagattt gaaagtgggt gacatcatca
    10801 caattgtcgg aaaacgtgcc gagtataatg gaaacccaca ggttggctct tctgtattgg
    10861 aaagctcgat tccggtaaca ccggcaacca tcgcagaggt gctggccaag ccagacgatc
    10921 ccaatacata ctttatggta accggcgaaa tcatatcgat tgccagtacc gaatggggta
    10981 atcttacctt gaaagatggc gatagtgaaa tctattcgta tggctgctat ccgggttatg
    11041 gcgcaaccgg cgatgccaga aaaaacctgt tggtggacaa aggtattaaa gtgggagacc
    11101 agctgacggt tattgcaccg aagaccactt atcaaggaac cccacagctt tcacgtagta
    11161 tttatttcag ccacacaagt gcgaagtaag taatgtgaca cgcagcataa gttgcgtgag
    11221 agataataag aggagctgcc tcatagcggg gcagctcttt ttttcaggct ggtatagatc
    11281 cctctaccct tttgtaagag ctgcttcata gcggggcagc tctctttttt cagacttgat
    11341 atagattctt ccaccctttt atgggggctg tctcaaagcg gggcaactct cttattatca
    11401 gcatgtcagg cgtggcagac ggagggaggt cgggttggcg ggcaagaaga gtgttttgta
    11461 agagacagag agatggtatc aacaaagttc ataaaagtta atatgtatgt taataaaaga
    11521 gaacttttat ccttgactgc tctgacccgc ttgcctattt tagcaaaacc cgttcatatt
    11581 tacggaaggt agactaacta acttattttc aaactttaat tttttttacg atcaatggga
    11641 gattcaattg tccattcggc taccgtcgcc tctaatgaaa aggaaatggt aaggaaagag
    11701 gagaagaaaa tcaaaaagga agagccggtg atgggtaaac ccagcaaaac tcagggtgaa
    11761 acgtcaaaaa agactttcac ctatgatgag gctttggagg ctacccgtca gtacttcggc
    11821 ggtgacgacc tggcagccaa ggtatggaca agcaagtatg ctcttaaaga ctcattcggc
    11881 aacctatatg aaaagacacc tgatgacatg cacaggcgtc ttgcttccga ggttcaccgg
    11941 attgagatga aatatcccaa ccctgtttcg tttgaggcta tttatgaagc attcaaggat
    12001 ttcaggtaca tccttccgca gggcagcccc atgaccggta taggcaatga tttccagatt
    12061 gcctcactct caaactgttt cgtgattggc aacaagggtg actctgactc atacgggggc
    12121 atcatgaaga ttgaccagga gcaggttcag ctgatgaagc gccgcggcgg tgtgggtcat
    12181 gacctctcgc acatccgccc gaaaggctca ccggtgctta acagcgccct cacctctaca
    12241 ggagtggtac ccttcatgga gcgctattca aactccaccc gcgaagtggc ccaggatggc
    12301 cgccgcggag cgctgatgct ctccatctca atcaagcacc ctgacgccgg gagcttcata
    12361 gatgccaagc tcgaagccgg aaaggtcacc ggcgcaaacg tctcggtgaa gattaccgat
    12421 gaattcatgc aggcggtgat tgacggaaag agtttccgcc agcaataccc catcaattcg
    12481 gatgatccaa agtatgtcaa ggatatagat gcccgcaagc tctggaacaa aattgtccac
    12541 aatgcatgga aatcagccga accgggagtg ctcttctggg ataccataaa gaaagaaagc
    12601 gtacccgact gctatgctga cctgggttat gagactgtat caaccaaccc gtgcggcgag
    12661 atacctcttt gtccgtatga cagctgcagg ctcattgcaa ttaacctctt cagctatgtg
    12721 aaggacccct tcacaccgaa ggcaagtttc gactatgatc tcttccgcag gcatgtgcat
    12781 ctctcacaac gcataatgga tgatatcatc gacctcgagc aggagaagat tgatgctatt
    12841 attgagaaga taaaagctga cccggaggac gatgagctca agagggtgga gcagaaaatg
    12901 tgggaaaata tcaggcacaa aaccggtgaa ggccggcgta ccggcatcgg catcacggcc
    12961 gagggcgaca tgcttgctgc actgggtctc cgttacggga gtgatgaggc aacggaattc
    13021 tcggtgaatg tacaccgtac cctggcaatc gaggcctaca ggtcgtcaac caaaatggca
    13081 actgaaagag gggcattccc catctacaac gctgaaaggg aaaagaataa tccctttatc
    13141 aaccgcatgc gtaaagcaga cccggagatg gttgccgaaa tggaaaaatc cggcaggcgc
    13201 aacatcgcac tgctgaccat agctcctaca ggttcaacca gccttttgag ccagacaacc
    13261 tcgggcattg aaccggtgtt catgcccttc tacaagcgtc gccgcaaggt taacccaagt
    13321 gacaaggacg tgaaggtgac cttcattgat gaggtgggag accactggga ggagtttacc
    13381 gtattccacc ataagttcat tgactggatg aaggtgaacg gctacaacta tgatgaggtg
    13441 aaggatatgt cagaagcacg gataaatgac attgtcgctg cctctcccta ttataaggca
    13501 acagcaaatg atgtagactg ggttgcaaag gtgcggatgc agggagccat acagaaatgg
    13561 gttgaccact ctataagcgt caccatcaac cttccctcag gggtgaagga agatctggta
    13621 tctgaactct atctgaccgc ctggaaggag ggttgcaagg gagcaaccgt ctaccgtgac
    13681 ggatccaggg caggtgtgct tgtctccaac agcagggagg agaagaagag caactggtcg
    13741 cctttaagac ccaaggtgct cgaggccgat gtcatccgct tccagaacaa cgaggaaaaa
    13801 tggattgcat ttgtcggtat caaggaccac gtaccatacg aaatattcac cggtctccag
    13861 gatgatgaga tgttccccat tcccaaaagt attatcaagg gtaagatcat caagaacaag
    13921 gatgatgcgg gcaatacgcg gtacgacttc cagtacacgg acaaatacgg ttataagaac
    13981 actcttggcg gcctgtcaca catgttcaag ccggaattct ggaactatgc ccgcctgata
    14041 tcgggtgtgc tacgctacgg gatgccggtg agtgacgtgg ttgacctcgt ctcgtcgatg
    14101 aagcttgaca gcgaatcaat caacacatgg aagaacggtg ttgagcgttc gctcaagcgt
    14161 tatatcccga atggcacgaa ggcaaagggc aagtgcgaaa actgcggctc aacctcactt
    14221 gtctaccagg agggttgcct gatatgcact gactgcggaa cctcgaagtg caactgagca
    14281 cttattaagc atcatatcac atttaaggaa gagtgtctca aaatataaat ggggcactct
    14341 tttttttggt aatgtggtta aaaagagctg gtgattttca gggagtaagg acaatcatca
    14401 gcggccggtg gtcagagatg accacttcat aatcagggta gcagggactg gcctttataa
    14461 ccattgtggt atcgatggat tcgaacagtt catcagttac cagtatatgg tctatatggc
    14521 ttggccacga tggataggac caccatagtt tgcttcccag ggcgatttcc atatcggcaa
    14581 acctgtatga tgaatcatca ttgatgaagt taaggaaggg gtttacagtt gctgaaccgg
    14641 atgcaatctc atcattgtaa tcgcccagca tcacgacggc gtcatcaggt ctttgctggt
    14701 caaggtattc cttcagcttt tccgatgctg agcggcggcg gttttcgtta tcctcgccct
    14761 cgcagcactt caggtggagg ttgataagga ttatttccct tcctgatttc ctgtgcctga
    14821 ccattatctc catcggaggg cgggggaagg catagcggtc accttcgaaa agggtccgca
    14881 gcgaaccggc tactgtttcc agttcactta ccctgatcag gtatgccagg ttccattctg
    14941 cattgtttat cggatagaag actccgctcc atcccggcat cagttccacg agcctgttga
    15001 attcagtttc ggaggcaacc tcctggagtg ccaccacatc agggtccatg gcctctaaca
    15061 ggtctgccag tgttgtgatg cttaaatatc cttctttcgg gaatccctga acgttgaagg
    15121 atacgatgtc cagggtgtta tccgtgccgg ggataacgca ggcggcaaag gggttgctgt
    15181 catcgggtaa cggaggatcc tgtatcctgc ggcaggaaat tacaacggtc agtgcagcaa
    15241 gggtgaccag taatatcagt ttatgaaaat gatttcccaa cataccctgt aaagttggca
    15301 atttttaatt catcaggcaa tagcagtcat gggcgacagg gaaaaaaagc tgtcccaatg
    15361 cggaaaacag gaagaaatga agagtcatcc cccaaaaaat gacaccctct tccagaacgg
    15421 aagtccgcag gcggaagtcg acaaccggta ttcaggagcc tgcagtctgc agtcggtatc
    15481 ctgtagcctg caaccggcag tcagcaacct gcacttccgg atatgaagta ccggggcagc
    15541 aat
//
LOCUS       DAOPBK010000045        15525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3875_length_15525_cov_5.009114, whole genome shotgun sequence.
ACCESSION   DAOPBK010000045 DAOPBK010000000
VERSION     DAOPBK010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_15525_cov_5.009114"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLA17_04130"
     CDS             complement(<1..257)
                     /locus_tag="PLA17_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPK84634.1"
                     /translation="MPVYDISPGVKGLIFDLDGTLADTMPWHFRAWQKACSRFGITME
                     TEFLQAHTGAPGWEIAGEVIEINGKRGMISPEEILKMKMEE"
     gene            complement(431..2464)
                     /locus_tag="PLA17_04135"
     CDS             complement(431..2464)
                     /locus_tag="PLA17_04135"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPK84635.1"
                     /translation="MTKNVNERAADNIRILSMAMVEKANSGHPGGAMGGADFIHILFS
                     EFLRFDPADPEWINRDRFFLDPGHMSPMLYSVLTLTGHFSIDDIMQFRQWGSVTPGHP
                     ELDVKRGVENTSGPLGQGHTMAVGAAIAEKFLATRFGRLFEHKIYAFISDGGIQEEIS
                     QGAGRLAGHLGLSNLIMFFDSNDVQLSTKTSEVTSEDTAAKYKAWGWRVMEVDGHSHD
                     QIRGALRMAVDENEKPVLIIGRTVMGKGLIDEAGNSFEEKVSTHGQPVSKAGASFKKS
                     VASLGGDPGNPFAIFSDVRQLYAEVIKNKKAAADAWKQKKETWAAANPDKARLLGSFY
                     AGVIPEINFAAVAQKENDATRNASGVLLSLFAEKIPNMIVASADLANSDKTDGFLKKS
                     KPFVRGDFSGAFLHAGVSELTMAAIMNGMALHGGVIPVCGTFFVFSDYMKPAVRLAAL
                     MGLPVKYVWTHDAFRVGEDGPTHQPVEHEAQIRLMEHLKNHHGENSMLVLRPADAIET
                     NIAWKLALENRTTPTALILSRQNIRTLPAPGTTRPDAAAMAARGAYTVTESDGSPDVI
                     LVASGSEVATLVEAALLLQADGIKTRIVSAPSEGLFRIQDEQYRHSVITPGIPVFCLT
                     AGLPVTMLGFAGSGGKIWGLESFGFSAPYGVLDQKLGFTGENIAGEVKKLLEK"
     gene            complement(2526..3974)
                     /gene="gnd"
                     /locus_tag="PLA17_04140"
     CDS             complement(2526..3974)
                     /gene="gnd"
                     /locus_tag="PLA17_04140"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HPK84636.1"
                     /translation="MKADIGLIGLAVMGENLVLNMESRGYTVAVFNRTTEKVSSFVNG
                     RGKGKKLIPAYSPGEFIASLSRPRKVMLMVKAGKPVDDFIELLIPLLDKGDIIIEGGN
                     SHFPDTIRRTAYVESKGLLYIGTGVSGGEEGALLGPSLMPGGSVEAWPHVKDIFHSIA
                     ARASDGAPCVTWIGDNGAGHFVKMAHNGIEYGDMQLICEAYHLMLSSGAFTYDEMADI
                     FEGWNKGELDSFLIQITGEILRFRDTDGQPLVTRILDSAGQKGTGKWTVISALDHGVP
                     LSLIGESVFARFISAMKKERVKASPLFNNPAGVTIRDKKQFVDDIRRALYASKIVSYA
                     QGFNLLRNAAAEYKWNLNYGEIAMIWRGGCIIRSAFLNKIYEAFSREPGLPNLITDEY
                     FTGILRENSDSWRRVVAHAVATGIPVPAMSSALAWFDSYRSEWLPANLLQAQRDYFGA
                     HTYQRTDKPEGEFFHTNWTGRGGDTASSTYNA"
     gene            complement(3991..>4668)
                     /locus_tag="PLA17_04145"
     CDS             complement(3991..>4668)
                     /locus_tag="PLA17_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3410 domain-containing protein"
                     /protein_id="HPK84637.1"
                     /translation="EAGRGGTEFHPLRTLLTDCDIITFHVPLTREGDDKTFRLIDRDI
                     LSRPGRQKIIINSSRGEVVDNLALREALKSGRVRGAALDVWEGEPAADPQLIELADIA
                     TPHIAGYSVDGKANATISSVGAVAAELGLPMHDWAPAELPQPAMPLIDLTGKGGATAV
                     EIVAHAVKHTYNVKEDDLLFRNNRENFEYLRDNYRIRREFSSYRVKTNDPEAERIMEK
                     LGFQIIK"
     assembly_gap    4669..4768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4769..5199)
                     /locus_tag="PLA17_04150"
     CDS             complement(<4769..5199)
                     /locus_tag="PLA17_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythronate-4-phosphate dehydrogenase"
                     /protein_id="HPK84638.1"
                     /translation="MKIVIDENIPFIRGAFERWADVVYAPGRSIDARMVADADALIVR
                     TRTRCDAALLKGSSVRIVASATIGFDHIDTLWLEQNGIKWANAPGCNSGSVMQYITSL
                     LFFLAEKHSFDPCSVTLGIVGVGNVGSKVARAAGAIGMRVL"
     gene            complement(5201..6472)
                     /locus_tag="PLA17_04155"
     CDS             complement(5201..6472)
                     /locus_tag="PLA17_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPK84639.1"
                     /translation="MVYYSEYRPDNGFDRDKIRAALESILGSFSERKRMLILPPDITR
                     AHSGAGMITELLREKLGERITDILPALGTHTPMETAELSRMFGRTPQTLFREHRWRTD
                     IEELGRVPASFVSSVTGGRLNFDYPVQVNRLLSSGGHDLIISVGQVVPHEVTGMANYN
                     KNVFVGCGGSEAINKSHFIGAVHGIENILGTTDNPVRAVLNKASEIAVPRLPLLYILT
                     VVAPDNQGCLKIMGLFAGDDIECFRQAAALSHELNFTIMPRRLKKVVVWLDPEEFKST
                     WLGNKSIYRTRMMIADDGELVVLAPGVRTFGEDPAIDRLIRKYGYRTTPEILAFTEAN
                     DDLKENLSAAAHLIHGSPENRFRVTYAAGHLSRAEVESAGYDFGDIDLLMKRYDPRSL
                     RTGFHTTADGEEFYFISNPATGLWKAAEQEQ"
     gene            complement(6536..7381)
                     /locus_tag="PLA17_04160"
     CDS             complement(6536..7381)
                     /locus_tag="PLA17_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK84640.1"
                     /translation="MNDFSDLRGKVAAITGGSGIIGKTLARGLCNAGINAIILDYKPE
                     RGKLTAKEISAGAEGGTAICFAADVTDRESLIRAREKIREKFGALDFLINCAGGNKAT
                     ATTAKEVIAESEKELKGTFFDLDIGGFDGVFDLNFKGTVLPTMILAEDMIRRGSGVIL
                     NISSMNAYRPLTKIPAYSAAKAAINNFTQWLAVHFAPAGVRVNAIAPGFLLTNQNRFL
                     LTDEKTGEMTPRGRKIISATPMGRYAEPEELIGTMLYLLSDASSFVTGVVIPVDGGFS
                     AFSGV"
     gene            complement(7394..8896)
                     /locus_tag="PLA17_04165"
     CDS             complement(7394..8896)
                     /locus_tag="PLA17_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK84641.1"
                     /translation="MTNYRWAITSLLFFSVVINYMDRQVLSLTWKDFIAPEFHWTNND
                     YGTITGLFSIFYAVCLLFAGRFVDWIDTKKGFLWAIGIWSAGAVLHAFCGIATSGIVA
                     GKWLVGFEEARRIISTVDDIGMVTSVSVTLFIFARFVLALGEAGNFPAAIKTTAEFFP
                     KKDRAFSTSIFNSGAQVGALIAPITVPVIAAKYGWEMAFIAIGAIGFIWMGFWTFMYK
                     KPHEHPRVNKAELDYINQDIIAETKANNNIAKPEKKISFLQTFKYRQTWAFAFGKFMT
                     DGVWWFYLFWTPAYLSDVYGLTSDTTMAKTLIFVLYAIVMLSIIGGWLPKYFVDKLGM
                     NPYAGRMRAMLIFAFFPLLALLAQPLGHISYWFPVIIIGIAAAAHQAWSANLFSTIGD
                     MFPKSVIATVTGIGGMAGGVGAFIINKGSGALFDFASGSTMVDGQVAVMTRELLAAGA
                     QYLNGPIKFFGFEGIRAGYFIVFCICAVAYLIGWVAIKMLVPKYKPIVID"
     gene            complement(9102..10508)
                     /gene="uxaC"
                     /locus_tag="PLA17_04170"
     CDS             complement(9102..10508)
                     /gene="uxaC"
                     /locus_tag="PLA17_04170"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPK84642.1"
                     /translation="MKEYINDDFLLQSESARKLYHEHSEGFPIIDYHCHLSPEAIAND
                     RRFEDLGQLWLEGDHYKWRAMRANGIDEKYITGKDTSFREKYDRWAETVPMTMRNPLY
                     AWTHLELKRVFGVGRLLNPDSAAEIWEECTALLQQPGYSARGLMKKFNVEVVCTTDDP
                     IDSLEHHKTIKNQGFEIRVLPAWRPDKAMSPEDPAKFIDYINELEETSGTEIRGFASL
                     IEALRVRHAYFAEAGCVISDHGIEQFHADDYTEKEVGYILRKVLSGSHLENSEISKFK
                     SAMLYELAVMDSERGWVQQFHYGAIRNNNSRMLAQLGPDAGYDSIGDFTVARSLSKFL
                     DRLDAGGNLAKTILYNLNPRDNELIATMAGNFQDGPVPGKIQFGAGWWFLDQETGMIN
                     QLNTLSLMGLLSRFTGMLTDSRSFVSYPRHEYFRRILCNMLGDDIEKGKLPESEMKTI
                     GKMVEMISYYNARDFFGF"
     gene            complement(10888..11778)
                     /locus_tag="PLA17_04175"
     CDS             complement(10888..11778)
                     /locus_tag="PLA17_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84643.1"
                     /translation="MNTYQNHPLAGAVDLDSAFNKLWYFYKKYFVGLYIISVVGSLVS
                     SIFISGLDLASLQTTTDPVEMLEIMKGFAGPYALALLVALVLSVLLHAWVLERPAGEA
                     GFISALLKKSLVAMVPYMIAMIIFGVMAVMLVSIGIVLLVLPGLFAIFYMATVGVFVM
                     PVMLAETRNPLEALTRSFKLAHKNLWTNIGWVIVVILILIVASLVLGAIVMLPFTGSF
                     VKSIANPEEASAMLDMARNPVYIGLSALTGGLVTPVMPILAFLLYFRNRGDEVAVEVT
                     TDDDNTVKVEDLYPPMPPGE"
     gene            complement(11829..14798)
                     /locus_tag="PLA17_04180"
     CDS             complement(11829..14798)
                     /locus_tag="PLA17_04180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK84644.1"
                     /translation="MHPTIVAARERLFALILTQHRRWGTILMPYMLEKMAGREYYTAE
                     EALSPFPNSFTLSELDNEQKEAVRLVNEYSDRNLFKLFSKHRNVREFLENVSEKEFSE
                     LIKPFIESRIAQCLRITLNEEIPLFLQKPGVNTLHHEDRLEINPLTADPVFRFDRTPD
                     ETAYSLIVESAGKPVNLRGQQIEILSNRPCIIRSGHTIHFIAEIEGIKIKPFLTRERI
                     IIPEKTVEKYFSTFVLQVINNHRVTGRGFSFKHSEAEKKAFLTVERTITGVPAAILKF
                     RYDNRDIYYPDDITHFTAFSNKEGHFIFLRKERDTAWEKSCLDALDEAGLTSDDGINF
                     TTPWSRESEGEAMYNLIETVSYNRKRLEDAGMIIRTGYLDRPFSLHRIEVRISHTVIN
                     DWFDLKAEVIIGPFTIPFTRFRRNILEGIREYPLPDGTVAILPEEWFARYRGLFEMGT
                     ASDDGLLLHKQHFSILSEAVSSEECDLCTGLAKLVMPEKLEILPAPEGLKAELRPYQK
                     EGMSWLHFLQSNNLGGCLADDMGLGKTLQALALLMYNRERMKTRMISRRCPESGADGS
                     DRMQHTLFGRETPPATSLIIVPASLCHNWHNEIKRFCPSMKTLTHYGASRQKSASHFT
                     SYDIVLSSYHTVRQDIELFTRVCFFYVVLDESQHIKNPSSHLYRSVMRLRAEHRLVLT
                     GTPVENSLTDLWTQLNFVNPGLLGSLAFFRREFARPIEKNNEEEKENRLRKIIRPFIM
                     RRTKEMVVSELPPVTEQVIQCDMSDEQAAVYEKEKSAVRNAILENIESVGIEKSAIIV
                     LQGLMRLRQLANHPVMTDENYRGGSGKFDAVTHNIENVVAEGHRILIFSSFVKHLELF
                     PGWLDKNGIGYTMLTGSTRERGKVIGSFRNNENIRVFLISLKAGGVGLNLTEADYVFI
                     LDPWWNPASEIQALSRAHRIGQEKRVFVYRYISGNTIEEKIQRLQEKKSRLAGTFIDV
                     NNPLSNLGVNEMLEIIN"
     gene            15015..>15525
                     /locus_tag="PLA17_04185"
     CDS             15015..>15525
                     /locus_tag="PLA17_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HPK84645.1"
                     /translation="MKKVIITGILLSLTLLLFSQETPVTTGWRAIRASKVSVDGRALT
                     TDDPAPEGWINATVPGTVLTTLVNNSIMPEPWYGMNNEKIPDVWDVGRDYYTYWFFTR
                     FSIPDLDSTRQVWLNFRGINYRAEIWFNGSLLSDTATDGMFLRRKYNITSLLNHDSHN
                     RLAVKVEPPL"
BASE COUNT         3515 a         4020 c         3864 g         4026 t
ORIGIN      
        1 aactcctcca tcttcatctt cagaatctct tcaggagaaa tcattcccct cttgccgtta
       61 atctctatca cctccccggc tatctcccag ccgggtgcgc cggtatgggc ctggagaaat
      121 tcagtctcca tagtaatccc gaaccgcgag catgccttct gccatgcccg gaaatgccat
      181 ggcatggtat cggcgagggt tccgtcaaga tcgaagatga gtcctttgac tccgggtgat
      241 atgtcgtaaa caggcattgg ttataattat gtcagaaatc aacagtgggc agtgtcagtt
      301 aatggtctgt ggttagaaat tatttgatta tatggttgtc agttgcattg caaagctgat
      361 gacgataatg tttatccggt tatgaaaagt gcggctccgg ctggagaccg cactcaggtt
      421 cgcagatccg ctatttttca agcaatttct tcacctctcc ggcaatgttt tcgccggtaa
      481 accccagttt ctggtcaagt acaccataag gagcagagaa gccgaagctt tcaagccccc
      541 agatctttcc gcctgatccc gcaaagccga gcattgtcac cggcagtcct gccgtcaggc
      601 agaagaccgg gatgcccggg gtgatgacgc tgtggcggta ttgttcatcc tggatgcgga
      661 agagtccttc cgaaggggca gatacaattc ttgttttgat tccgtcagcc tggaggagca
      721 atgctgcctc caccagcgtt gccacctctg atccgctggc aaccagtatg acatccgggc
      781 tgccgtcact ctctgtcacg gtgtaggcgc ctcttgcagc cattgcagca gcatctggcc
      841 gtgttgtgcc cggtgccggg agagtcctga tgttctgccg tgaaagtatc agggctgtgg
      901 gggttgtcct gttttccagg gccagtttcc aggcgatatt cgtctcgatg gcatcagccg
      961 gacgaagcac cagcatgctg ttttcaccgt ggtggttctt aaggtgttcc atgagacgta
     1021 tctgcgcctc atgttccacc ggctggtgag tgggaccatc ctcgcccaca cggaaagcgt
     1081 catgggtcca tacatatttg accggcaggc ccatgagcgc agccagcctt actgcgggtt
     1141 tcatgtagtc agagaagacg aagaatgttc cgcagacggg aatgacacca ccgtgcaggg
     1201 ccatgccgtt cattatggcg gccatggtaa gctcggagac gcccgcatga aggaaggcgc
     1261 ctgagaaatc tcccctgaca aaaggtttgc tctttttgag gaatccgtca gtcttgtctg
     1321 aattggcaag gtctgcagat gcaacaatca tgttgggtat cttctctgca aagagggaga
     1381 ggagaacacc tgaagcattc ctggtggcat cattttcctt ttgcgccacg gcggcgaagt
     1441 tgatttccgg gatgactccc gcatagaagc tcccgagaag acgggcctta tcgggattgg
     1501 ccgctgccca tgtctccttc ttctgcttcc aggcatctgc agcagccttt ttattcttga
     1561 ttacctctgc gtacagttgc ctgacatcgc tgaagatggc gaaaggattc ccgggatcac
     1621 cgccgaggct ggccactgac tttttgaatg aagctcctgc ctttgagaca ggctgtccgt
     1681 gggttgatac cttttcttcg aagctgttac cggcttcatc aataaggcct ttccccatca
     1741 ccgtccggcc gatgataagg acgggcttct cgttctcgtc gacagccatc cggagtgcac
     1801 cccgtatctg gtcatgactg tggccgtcta cctccatcac ccgccagccc catgccttgt
     1861 acttggctgc ggtatcctca gaagtcactt ccgaagtttt ggttgagagc tggacatcat
     1921 tggagtcaaa gaacataatc aggttgctca gcccgagatg acctgcaagc ctccctgctc
     1981 cctgggagat ctcctcctgg attcctccgt cagagataaa tgcatatatc ttgtgttcga
     2041 acagccgtcc gaacctggtg gcaagaaatt tttccgcgat agcggcgccg acagccatgg
     2101 tgtgaccctg tccgagaggt ccggaggtgt tctccacacc tctttttaca tccagttcgg
     2161 gatggccggg cgtcacgctt ccccactgcc ggaactgcat gatgtcatca atggaaaaat
     2221 ggccggtcag tgtcagtacc gaatagagca tcggcgacat atgtccggga tcaaggaaaa
     2281 agcggtcacg gttgatccat tcagggtcag ccggatcaaa tctcaggaat tcagaaaaga
     2341 ggatgtgaat aaaatcagct ccgcccattg ctcctccggg gtgcccggag ttggctttct
     2401 ccaccatagc cattgaaaga atacggatat tgtctgctgc gcgttcgttt acgtttttgg
     2461 tcataataat tcaagcctta atgttgaagg gtaaaaatat agaaaaaagt ggtacctgag
     2521 cctgttcagg cattataggt tgaggaggcg gtatcacctc cgcgtcccgt ccagtttgta
     2581 tggaagaatt caccttccgg tttgtcggtc cgctggtagg tatgtgcccc gaaatagtca
     2641 cgctgtgcct ggaggaggtt ggcaggcagc cactctgacc ggtagctgtc aaaccaggcg
     2701 agggccgagc tcatggcggg gacgggtatg cctgttgcca ccgcgtgggc caccacgcgg
     2761 cgccagctgt cactattctc cctgagtatc cctgtgaagt actcgtctgt gatcaggttg
     2821 ggcaggcccg gttcccggct gaaagcctcg tatatcttgt tgaggaaggc tgaccgtatt
     2881 atgcaccctc ccctccatat cattgctatc tctccgtagt tgaggttcca tttgtattct
     2941 gcggcggcgt tcctgagcag gttgaaaccc tgtgcatagg agacgatctt tgatgcgtac
     3001 agtgctctgc ggatatcatc aacaaactgt ttcttgtccc tgatggtcac cccggccgga
     3061 ttgttgaaga ggggagaggc tttcaccctc tcctttttca ttgctgagat gaaacgggcg
     3121 aagaccgatt cgccgatgag gctgagaggc acaccatggt caagggcgct gatgacagtc
     3181 catttgcctg ttcccttctg gcctgccgag tcaagtatac gggtgaccag tggctggccg
     3241 tcggtgtctc tgaaccggag tatctcaccg gttatctgta tgagaaagga gtcaagctca
     3301 cccttgttcc acccctcgaa tatatcagcc atctcatcat aggtgaaagc tccggaactt
     3361 agcatcaggt ggtaagcttc gcatatcagc tgcatgtcgc catactcgat gccattgtgt
     3421 gccatcttta cgaagtggcc ggcgccgttg tcacctatcc atgtgacaca gggggcgcca
     3481 tcagaagccc tggctgcaat ggaatgaaaa atatccttca catgaggcca tgcctccacg
     3541 gaaccgccgg gcatcagcga agggccgagc agcgcgcctt cttcacctcc tgaaactcct
     3601 gtgccgatat aaaggagacc tttgctttca acataggcag ttctcctgat agtgtcagga
     3661 aaatgagagt tgcccccctc tatgatgata tcacccttgt cgagcagcgg tatcagcagt
     3721 tcaatgaagt cgtcaaccgg tttgcctgcc tttaccatca gcattacttt tctcggccgg
     3781 ctgaggcttg caatgaattc cccgggagag tatgcgggga tgagtttttt tcctttgccc
     3841 cgtccgttca caaatgatga tactttttct gttgtgcggt tgaaaaccgc cacggtgtaa
     3901 ccacggctct ccatattcag taccaggttc tcacccatca ctgcaagccc aatcagtcca
     3961 atgtctgctt tcacgtagat atttttaatc tcatttaatt atctgaaaac caagcttttc
     4021 cataattcgt tctgcttccg ggtcattggt tttgaccctg tatgaggaga attcgcgcct
     4081 gattctgtag ttatcgcgaa ggtactcgaa attctccctg ttgtttctga agaggaggtc
     4141 atcctccttc acgttgtatg tatgttttac tgcatgtgcc actatttcaa cggctgtggc
     4201 gccgcccttg ccggtgaggt caataagcgg catggccggc tgcggcagct ccgccggggc
     4261 ccagtcgtgc attggcaaac cgagttctgc agccaccgcc ccgacggagc tgatggtggc
     4321 gttggccttg ccgtcaacgg agtaacctgc aatgtgaggt gtggcgatgt ctgccagttc
     4381 aatcagctgc gggtcggcag ccggctcccc ttcccatacg tcaagggcgg cgccgcgcac
     4441 tctgcccgac ttcagtgctt cacgcagcgc aagattgtct accacctcgc cgcgcgatga
     4501 gttgatgatg attttctgcc tgccaggccg cgaaaggata tccctgtcga tcaggcggaa
     4561 tgttttgtca tcaccttcgc gggtgagggg aacatgaaat gtaatgatat cacaatctgt
     4621 caataatgta cgtagtgggt ggaactcagt tccacccctg cctgcctcnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa cagtacccgc atgccaatgg cgcctgccgc
     4801 ccttgccacc tttgatccga cattgccaac gcccactatg ccaagcgtca cagaacaggg
     4861 gtcaaacgag tgcttctccg ccagaaaaaa gaggagggag gtgatgtact gcatcaccga
     4921 gcctgagttg cagcccgggg cattggccca tttaatccca ttctgctcca gccagagggt
     4981 atcgatatga tcaaacccga tagtggccga ggctactatc ctgacgctgc ttcctttcag
     5041 aagggcagca tcgcatctgg tgcgtgtgcg gacaataagc gcatctgcat ctgccaccat
     5101 acgcgcatca atgctgcggc caggtgcata aaccacatcg gcccaccgct caaaggcgcc
     5161 cctgatgaag ggtatatttt cgtctatgac aatcttcata tcactgttcc tgttcagccg
     5221 ctttccagag ccccgtagcg ggattggaga taaagtaaaa ctcttctccg tcagcagtgg
     5281 tgtggaaacc tgtccggagg ctccttgggt cataccgctt catgagcagg tcaatatcac
     5341 caaagtcgta gcctgccgac tccacctctg cccggctgag gtgaccggct gcatatgtca
     5401 ccctgaaacg gttctcgggt gagccatgga taagatgggc agcggcgctg aggttttcct
     5461 tcaggtcgtc attggcttcg gtgaatgcca gtatttcagg agtggtgcgg tatccgtatt
     5521 ttctgatcag cctgtcaatg gcagggtctt caccgaaggt tctgacaccg ggggccagca
     5581 ccaccagttc accgtcatcg gcaatcatca tacgtgtccg gtagatactc ttgttgccga
     5641 gccatgtgct tttgaactct tcaggatcga gccataccac cacctttttc agccttcggg
     5701 gcattatggt gaagttgagt tcatgcgaga gtgcggcagc ctgcctgaag cattcaatgt
     5761 catcaccggc aaacaggccc atgattttca gacagccctg gttatcaggg gccaccaccg
     5821 tgaggatgta gagcagcggc aaccgcggga cggctatttc ggaagctttg ttcagcaccg
     5881 ccctgaccgg gttgtcagtc gtgcccagta tgttttcaat accatgtacc gcccctatga
     5941 aatgactctt gtttatggct tcgctgccac cgcagcccac gaaaacattc ttgttgtaat
     6001 tggccatgcc ggtcacctca tgcggcacca cctggccaac ggagattatc aggtcatgcc
     6061 ctcctgatga gaggaggcgg ttaacctgca ccgggtaatc aaagttgagg cgtcctccgg
     6121 taaccgatga cacgaatgat gcaggcaccc tcccaagttc ctctatatct gttcgccaac
     6181 ggtgttccct gaagagtgtt tgcggcgttc ttccgaacat ccttgaaagt tcggctgtct
     6241 ccatgggcgt atgcgtgcca agggcaggga ggatgtcagt gatccgttca cccagcttct
     6301 cccggagcag ttcggtaatc atcccggcgc ccgagtgtgc ccgtgtaatg tcgggtggca
     6361 gtatcagcat ccgcttcctt tcgctgaagc tgccaaggat agattcaagg gcggctctga
     6421 ttttatctct gtcaaagccg ttgtcgggtc tgtattcgga ataatatacc atgttgctaa
     6481 cttcaacata attgctggtt ctttgtaaac aaaagaacca gttgcccaaa aatctctaga
     6541 ctccgctgaa tgcactgaaa cctccatcga caggtatcac taccccggtg acaaacgatg
     6601 aggcatcaga aagcagatag agcattgtgc cgattagttc ttccggctcc gcatagcgtc
     6661 ccattggtgt ggctgatatg attttcctgc cgcgcggagt catctcaccg gttttttcat
     6721 cagtcagcag aaaccggttc tgattggtca gcaggaatcc gggtgcgatg gcgttgaccc
     6781 tcactccggc gggtgcgaaa tggacagcga gccactgtgt aaagttgtta atggctgcct
     6841 tggctgctga ataggcgggt atcttggtta gcggcctgta ggcattcatt gaggagatat
     6901 taaggatgac gccactccct cttctgatca tatcttccgc caggatcatc gtgggcagca
     6961 ccgttccctt gaagttgaga tcaaagacac cgtcaaatcc tccgatatca aggtcgaaga
     7021 aagttccttt cagctctttt tcgctttccg caatgacctc ttttgccgtt gtggcggtgg
     7081 ctttattgcc tccggcgcag tttatgagga agtcgagagc accgaatttt tccctgattt
     7141 tctcgcgtgc cctgataagt gactctctgt cagtcacatc tgccgcaaaa cagatcgctg
     7201 ttccaccttc agctcctgca cttatctctt ttgcagttaa cttccccctt tccggtttgt
     7261 aatcaagtat tattgcatta atacccgcat tgcaaagccc cctggcgaga gttttcccaa
     7321 ttattccgct gccgccggtg atggcggcca cttttcctct cagatcactg aaatcattca
     7381 tcttactgtt tttctagtca attactatcg gtttgtattt cggaaccagc atcttaatcg
     7441 ctacccatcc gatgaggtaa gctacagcgc agatgcaaaa gacaataaag taacctgccc
     7501 tgatgccctc gaatccgaag aactttatgg ggccgttaag gtactgtgct ccggcggcaa
     7561 gcagttccct ggtcattact gcaacctgac cgtcgaccat ggttgagcct gatgcgaagt
     7621 caaacagggc gcctgagcct ttgttgatga tgaaggctcc gacgccgcct gccattccgc
     7681 ctatcccggt aactgtggcg ataaccgatt tcgggaacat gtcaccgatg gtggaaaaga
     7741 gattggcaga ccaggcctgg tgcgcagccg cggcaatacc tattataata accgggaacc
     7801 agtaggagat atgccccagc ggctgtgcca gcagggccag caacggaaag aatgcgaaga
     7861 tcagcatggc acgcatacgg ccggcgtagg gattcatacc cagtttgtca acaaagtatt
     7921 tcggcagcca gccacctatt attgagagca ttacaatcgc gtaaagcacg aaaatcagtg
     7981 tttttgccat tgtagtatct gatgtcagtc cgtaaacgtc actcaggtaa gccggtgtcc
     8041 agaagaggta aaaccaccat accccgtcag tcatgaactt tccgaaggcg aacgcccatg
     8101 tctggcggta tttgaatgtc tgcaggaaag agatcttttt ttccggtttg gctatattgt
     8161 tatttgcctt cgtctccgcg atgatgtcct ggttgatata gtcaagctct gccttattta
     8221 cccgggggtg ctcatgaggt tttttgtaca tgaaggtcca gaatcccatc cagataaacc
     8281 cgattgcgcc gatggctatg aatgccattt cccagccgta ttttgctgca atgaccggta
     8341 cggttatggg tgctatcagg gctcccacct gtgctccgga attgaatatg cttgttgaga
     8401 atgcgcggtc ttttttgggg aagaactctg ctgttgtctt gatggctgca gggaagttac
     8461 ccgcctcccc gagggccagc acgaagcgtg caaaaataaa gagtgtgacg ctgaccgaag
     8521 ttaccatccc gatatcatcc accgtactga ttatccttcg tgcctcctca aaccccacca
     8581 gccattttcc ggcaacgatg cctgatgttg ctatcccgca gaaggcatga agcacagcgc
     8641 ctgcagacca tattcctatt gcccagagga atcccttttt ggtatctatc cagtcgacaa
     8701 accttccggc aaacagcagg cagacggcat agaagatcga gaaaagaccg gttatggtac
     8761 cgtagtcatt gttggtccag tgaaactcag gtgctatgaa atccttccag gtcagtgaaa
     8821 gcacctggcg gtccatgtaa ttgatcacta ctgaaaagaa caacaatgag gtgatggccc
     8881 atctgtagtt tgtcatcctg gattgtgtgg tttctgaggc catattgttt tattaaatgc
     8941 ttatttcata tgttggtagt tgcggtcagg agcacctaac tgatcctttg caaaaatgag
     9001 ggtcagaatg acagaagagt ggaacaagga cggtctcatc cattaccagt gcaacattaa
     9061 tccgtttaaa acctgacatc atctatctct ttttggtcgt ttcagaaacc gaaaaagtcc
     9121 ctggcgttgt aataagagat catctcaacc attttaccta tggtcttcat ctctgattca
     9181 ggcagcttcc ctttttcaat atcatcaccc agcatattgc agagaattct tcggaagtat
     9241 tcatgcctgg ggtatgatac aaagctgcgc gagtcagtga gcatccctgt gaacctgctg
     9301 agaaggccca tcagtgacag tgtattgagt tggttgatca tacccgtttc ctggtcaagg
     9361 aaccaccagc cggccccgaa ctgtatcttt cccgggaccg ggccatcctg gaagttgccg
     9421 gccatggttg ctatcagctc gttgtctctc gggttgaggt tgtagagtat cgtttttgcc
     9481 aggttgccac cggcatcaag cctgtcgagg aatttcgaaa gtgatctggc aaccgtaaag
     9541 tcgccaatgg agtcgtatcc ggcatccggg cctaactgag ccagcatccg gctgttgttg
     9601 ttgcgtatgg cgccgtaatg aaactgctga acccatcctc tctcgctgtc cattactgcc
     9661 agctcataca gcatggctga cttgaatttt gaaatctcac tattctccag gtgtgaccct
     9721 gaaagcacct tccggaggat atatcccacc tctttttcag tgtaatcatc ggcatggaac
     9781 tgttcgatgc catggtcact gatgacgcat cccgcttcag cgaagtaagc gtgcctgaca
     9841 cgcagggctt ctatcagtga ggcaaatccc ctgatctcag ttcctgaagt ctcttcaagc
     9901 tcattaatat agtcaatgaa tttcgcagga tcttccggtg acatggcctt gtctggtctc
     9961 catgcgggca ggactctgat ctcaaagccc tgatttttta ttgttttatg atgttcgagg
    10021 ctgtcaatgg ggtcatcggt agtgcatacc acttctacat tgaacttctt cattagtccg
    10081 cgtgcagaat atccgggttg ctgaagaagt gccgtgcatt cttcccatat ttcagcagcc
    10141 gaatcagggt tcagaagcct gcccacgccg aagaccctct tcagctccag gtgggtccat
    10201 gcataaagcg gattgcgcat ggtcatcggc acggtctctg cccaacggtc atatttctcc
    10261 cggaaagagg tatcctttcc tgttatatat ttctcatcta taccgttggc acgcattgca
    10321 cgccacttat aatggtcgcc ctcaagccag agctgcccaa ggtcctcaaa gcgcctgtcg
    10381 ttggcaatgg cttcgggact gaggtggcag tggtagtcaa ttattggaaa tccttccgaa
    10441 tgttcgtgat agagcttgcg ggctgactca ctctggagca ggaagtcgtc gtttatgtac
    10501 tctttcatat cattcgtgaa ttggttttca gctttgtcgg aaaaatagca ataaaaaact
    10561 aaaccagagt ctcaaaacag caataattcg cacataaaaa aaggttcgta attactgcaa
    10621 caatggcact gcaatcggtt gccatgacag gccggtgcca gagcttccgg atcaatacca
    10681 tccagcatgg agacaatggt taccgatggg tatctcggca attttgtcat gaaacaatta
    10741 acatcctggc agcacagatg atcgccaccg gtagcccggc tgtcatggtg aagctcatca
    10801 cctgcaaaga gggcctgaag ggaagatctc cggcaggatg ctgaaaaggt tcagccggta
    10861 aaacaggtta ttccgttccg ggtgctgtta ttccccggga ggcattggcg ggtacaggtc
    10921 ttctaccttt acagtgttgt catcatcggt tgtaacctca acagccacct cgtcacccct
    10981 gttcctgaaa tatagcagga acgcaagtat cggcatgacc ggtgtcacca ggccgccggt
    11041 cagggcactg aggccgatgt aaacagggtt ccgtgccata tcaagcattg ccgaagcttc
    11101 ctcagggttg gctattgact tcacgaatga tccggtaaag ggaagcatca ctatagcccc
    11161 gaggaccagg gatgcaacta tcaggataag aatgactaca ataacccagc caatgttggt
    11221 ccacaggttt ttatgtgcca gtttgaatga acgtgtaagt gcctccagcg ggttacgggt
    11281 ttcggcaagc atcaccggca ttacaaatac cccgacagtt gccatgtaaa agatggcaaa
    11341 aagtccggga agaaccagga gaacaattcc gattgacacc agcatcacgg ccatcactcc
    11401 aaagatgatc atcgcaatca tgtatggaac catcgcaaca agtgattttt tcagcagggc
    11461 agatatgaag cctgcttctc ccgcaggcct ctccagtacc catgcatgca gcagaacgct
    11521 cagcaccaat gctaccagga gagccagggc atagggtccg gcaaaaccct tcattatttc
    11581 aagcatttcg acgggatcag tggttgtctg aagtgatgcc agatcgaggc ctgagatgaa
    11641 tatggatgaa accagtgatc cgactacgga tatgatatat agtccaacga agtacttctt
    11701 gtagaagtac cacagcttat tgaatgcact gtcaagatcg acagcccctg caagaggatg
    11761 gttctgatag gtgttcatca cagcgttttt agaagatttg taaattctgg ggtaaatcta
    11821 ctaaattatc agttaattat ctcaagcatt tcgttcacgc cgaggttgct cagcggattg
    11881 ttgacatcaa tgaaagttcc ggccagtcgt gattttttct cctgcagccg ctggatcttc
    11941 tcctcgatgg tattgccgga gatatacctg taaacaaaga cccttttctc ctggcctatc
    12001 ctgtgcgccc tgctgagcgc ctgaatctca gatgcagggt tccaccacgg gtcgaggatg
    12061 aatacgtaat cagcctctgt aaggtttagc ccgactcctc cggccttgag cgaaatcagg
    12121 aaaaccctga tgttttcatt atttctgaag gagcctatca ccttcccccg ttccctggta
    12181 gaaccggtaa gcatggtgta tcctatgccg ttcttgtcga gccatccggg gaacagctcg
    12241 aggtgcttga caaatgatga gaatatcagt atccggtgtc cctcagccac cacgttctca
    12301 atgttatgtg tcacggcatc gaacttgcct gagcctcccc tgtagttttc atcggtcata
    12361 acggggtggt ttgccagttg ccggagcctc atcagcccct gaagaacgat gatggctgat
    12421 ttttcaatgc caacagactc tatgttttcc aggatggcat tgcgtacagc agatttttcc
    12481 ttttcgtaaa cagcagcctg ttcgtctgac atgtcacatt gaataacctg ctccgttacc
    12541 ggaggaagct cactcaccac catctccttg gtgcgtctca tgatgaaagg ccggattatc
    12601 ttcctgagcc tgttctcctt ttcttcttcg ttgttctttt cgattggcct cgcaaattct
    12661 ctcctgaaaa atgcgagtga tcccagcaat ccgggattga cgaagttaag ctgtgtccac
    12721 aaatcagtca gtgagttctc taccggtgtt ccggtaagca ccagcctgtg ttcagcccta
    12781 agcctcatca ccgacctgta gaggtgtgag gaggggttct tgatatgctg gctctcatcg
    12841 agcaccacat agaagaagca aaccctggtg aagagctcaa tgtcctgcct gaccgtatga
    12901 taacttgaca gtactatatc atagctggta aagtgtgatg ctgatttttg ccgtgatgca
    12961 ccgtagtggg tcagtgtctt cattgacggg cagaaacgtt ttatctcatt gtgccagttg
    13021 tggcatagcg acgccggtac aatgatcagg gaggtggccg ggggagtttc cctgccaaag
    13081 agggtatgct gcatccggtc actgccgtca gcgcctgatt ccgggcacct cctgcttatc
    13141 atgcgggttt tcatcctttc cctgttgtac atgagcagcg ccagggcctg cagtgttttg
    13201 ccaagaccca tgtcgtcagc aaggcaaccg ccgaggttgt ttgactgcag gaaatgtaac
    13261 cagctcattc cctccttctg gtagggccgc agctctgctt taagtccttc cggtgcaggt
    13321 aatatctcca gcttctcagg catgacaagc ttcgccagcc cggtacagag gtcgcactct
    13381 tccgatgaga cagcctcgct caagatgctg aaatgttgct tgtgcagcag cagaccgtca
    13441 tcgctggcag tgcccatctc gaacagtccc cggtatctgg caaaccattc ctccgggaga
    13501 atggcaactg ttccgtcagg caacgggtat tcgcggatac cttcaagaat gtttctcctg
    13561 aaacgtgtaa agggaattgt aaacggtcct atgatcactt cggctttcag gtcgaaccag
    13621 tcatttatta ccgtgtgact gattctcacc tcaatcctgt gcagcgagaa gggccggtca
    13681 agatatccgg tacggattat catacccgca tcctccagtc tctttctgtt ataggagact
    13741 gtttcaataa ggttgtacat tgcttcccct tccgactccc tggaccatgg ggtggtgaag
    13801 ttaatgccgt catcacttgt caggcctgct tcatcaagtg catccaggca actcttttcc
    13861 catgccgtat cacgttcctt ccgcaggaag atgaaatggc cctccttatt gctgaatgct
    13921 gtgaaatggg taatatcatc aggataataa atatctctgt tgtcatagcg gaattttagt
    13981 atggctgccg gaactccggt gatggtacgc tcaacagtta gaaatgcttt tttctccgct
    14041 tccgagtgct taaaggagaa ccctctccct gtcacccggt gattgttgat cacctggagc
    14101 acgaacgtgg agaagtattt ttccactgtt ttctcaggaa tgatgatcct ctccctggtc
    14161 aggaaaggct tgattttgat cccttcaatt tcagcgatga agtggatggt atgccccgat
    14221 ctgatgatgc atggcctgtt tgacagtatc tcaatctgct ggccgcgaag attgactggt
    14281 ttgccggctg attccacaat caggctgtac gcggtttcat cgggagtacg gtcaaaccgg
    14341 aacacgggat ctgccgtcaa tggattgatt tccagtctgt cttcatgatg aagggtgttt
    14401 actcccggct tttgaaggaa gaggggtatc tcttcattca gtgttatccg gagacactgt
    14461 gcaatcctcg actctataaa cggctttatc agttcgctga actctttctc gctaacattt
    14521 tccaggaact ccctgacatt ccggtgtttt gaaaaaagct tgaagaggtt gcggtctgag
    14581 tattcgttaa caagccgcac cgcctccttt tgctcgttat caagttctga aagggtaaag
    14641 ctattgggga atggcgacag cgcctcttcc gcggtgtaat actcccttcc cgccatcttc
    14701 tcaagcatgt atggcatcag tatggtgccc catctgcggt gctgggtcag aataagtgca
    14761 aacagccttt ccctggctgc tacaatggtg gggtgcatct gtaaacgaaa taaacatgca
    14821 attttgaaat tccccgggta aaatcaaata gctggcatta aattattatt tacccggggc
    14881 tggtcaattt acctgtgact ttgtcgtttt taagcgctga tgatgagtct tttacggcat
    14941 ttgtgtcctg cctgcagcag tgtgaaagga taattttaat atctttccgt acttgtttta
    15001 taagggaaca acaaatgaaa aaggtcatta taaccggtat tttattgagt ttgactttgc
    15061 tgctcttctc tcaggagaca cctgtcacaa ccggatggag ggcaatccgc gcatcgaaag
    15121 tatctgttga cggcagggcg ctgaccacag atgaccctgc accagagggg tggataaatg
    15181 ccactgttcc cggcactgtc ctgactaccc tggtcaataa cagcatcatg ccggaaccat
    15241 ggtacggaat gaataacgag aaaattcctg atgtatggga tgtgggccgc gattattaca
    15301 catactggtt cttcacaaga ttctcaatac cggaccttga ctccacccgc caggtgtggc
    15361 tcaatttcag ggggataaac tacagggccg agatatggtt caacggcagc ctcctcagtg
    15421 acaccgctac tgacggtatg tttctccgca ggaaatacaa tataacctcc cttctgaacc
    15481 atgacagtca taaccggctg gcagtcaagg tagaaccgcc gctta
//
LOCUS       DAOPBK010000046        15442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3905_length_15442_cov_6.257750, whole genome shotgun sequence.
ACCESSION   DAOPBK010000046 DAOPBK010000000
VERSION     DAOPBK010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3905_length_15442_cov_6.257750"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /gene="nadA"
                     /locus_tag="PLA17_04190"
     CDS             complement(<1..315)
                     /gene="nadA"
                     /locus_tag="PLA17_04190"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPK84646.1"
                     /translation="MVVDNNIIEKIKALRKEKNAVIFAHYYQTSDIQAIADFIGDSLA
                     LSQKAAETTADIIVLAGVKFMAETVKILSPEKKVIIPDLMAGCSLADSCKKEDFEEFI
                     NDN"
     gene            765..1205
                     /gene="rpiB"
                     /locus_tag="PLA17_04195"
     CDS             765..1205
                     /gene="rpiB"
                     /locus_tag="PLA17_04195"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPK84647.1"
                     /translation="MEKIIIASDHAGFRLKEKLVSWLITSRYEVRDIGCFSEDSVDYP
                     DYAHPLAVAVENGEYDYGITICGSGNGISMACNKHASIRSAVCWNNEISRLARSHNDA
                     NICALPGRFLTEAEAIQIVKVFLTTSFEGGRHKRRIDKIPIKTV"
     gene            1206..1646
                     /locus_tag="PLA17_04200"
     CDS             1206..1646
                     /locus_tag="PLA17_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPK84648.1"
                     /translation="MLSATCKYGIRAVLYIGSKPDTKVNVGLKEISENLKIPQPYLAK
                     ILQVLARKKILRSTKGPHGGFYLLVPAEKLTLMDIIDAIDGRDFFDSCYVTGERCNFD
                     KPDDGRCILHNDLREEKMRLEKFFSSKTVDQIIARVRNDPSVKI"
     gene            complement(1618..1920)
                     /locus_tag="PLA17_04205"
     CDS             complement(1618..1920)
                     /locus_tag="PLA17_04205"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPK84649.1"
                     /translation="MLNIPYLDKMGHFVMYMFFSAILLLDSCRWQTSRNLRYIILLIP
                     LFFGALMEILQMTTTTRKAECMDMAANIGGIVAGILLAHIALKILERFRSSQTDHS"
     gene            2096..3226
                     /gene="hemW"
                     /locus_tag="PLA17_04210"
     CDS             2096..3226
                     /gene="hemW"
                     /locus_tag="PLA17_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPK84650.1"
                     /translation="MAGIYIHIPFCKSFCNYCDFYSITDDSLKGDFVRALIREASLRS
                     SYLEGETVETIYFGGGTPSLLEPATAAAIINDLRKIFPFSGDPEITFEVNPDDVSEGM
                     PGELKKAGVNRISLGVQSWDDRSLRYLGRRHDAAQSVRALEMIFGEGINNVSVDIIYG
                     VPGTTTVDLKADLEKTLVFPVTHLSAYHLTVEDGTRFGRMKKEGKLKEIDEESSSAMY
                     SLLVRYCREHGFVNYEISNFAKEGFISRHNSSYWKQIPYLGLGPSAHSFDRRSRQWNV
                     SDVRKYIRAVSSGEIPCEREELDKIMMFNEYVMTTLRTMWGIDLIHVEEFYDKELHDY
                     LVNLSGKYITYGLMKREKNTLVLTDQGRMMSDNIIAELLADL"
     gene            complement(3227..4135)
                     /locus_tag="PLA17_04215"
     CDS             complement(3227..4135)
                     /locus_tag="PLA17_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84651.1"
                     /translation="MENQEKYQAEHVEHKKGAPVVMIVSTIILTLGLIALVVLYYNQK
                     SKMVEMEQVLTEEKDSLANELRMMMYGYDTLKTSNDTLNAELLRERTRIQKLLEVNAS
                     NAQLIRKYKAEISTMREIMKSYIVQIDSLNTRNQMLTAENVEIKQQMTAVQETNVELE
                     KVKEELTSKVTVASMIQAKDVVAVALNKKRKETTELRNLDKVRVCFTLRENPIASAGN
                     KVVYLRVVRPDQLVITPSPDNLFEVKGEQLIFSANRAVDYANTDIEMCIFLDNTGDFI
                     TGTYDVELYLDGEKIGTGAFSLKGKG"
     gene            4250..5653
                     /locus_tag="PLA17_04220"
     CDS             4250..5653
                     /locus_tag="PLA17_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPK84652.1"
                     /translation="MSGFYGSVSKRSCIDDVFYGTDYHSHLGTKRAGLVFYDREIGFR
                     RRIHNLENEYFRSKFEAELKEFKGNSGMGIISDTDPQPILFDSHLGRFALVTVSKIVN
                     INELADYFLARKKHFTENFMGNINPTEMVANLICEGDSYVEGIENVFDKIQGSCSLLL
                     LTTDGIIAARDKLGRTPVVIGMKEGARAVVSETCSLPNTGFETEYYLGPGEIVHIRAS
                     GFTRLKAPGRRMQICSFLWVYYGFPTSEYEGRNVEQIRYGCGAALARRDPVKADLVCG
                     VPDSGVGHAIGYSNEKQLPYMSVFSKYTPTWPRSFTPQRQVQRELVAKMKLIPNKKLI
                     KGRKIIFLDDSIVRGTQMRNNTRDLRDAGAAELHMRIACPPLLYPCQFLNFSQSRSTL
                     ELAARKAIIRLEGDEKKVEEYLDPDSEKYGKMVKEIARDLGMDSLMFQRLDDLVKAIG
                     LPREKLCTHCWDGSGKF"
     gene            5953..7242
                     /locus_tag="PLA17_04225"
     CDS             5953..7242
                     /locus_tag="PLA17_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK84653.1"
                     /translation="MKIPGITDLKPLSLFFIYLLLLSEINLSAQDTVSVRLNLKECIE
                     FGLKNNPGLQSSIYLVEEMKAKTDEALSGYYPAVSLNSDADTYSKNNGSQRYNNISSG
                     INFSYNIFQGNRIKATYGASQDNYQASRYNHETLRQDLVFNIIKAYYRILQSEWILRS
                     SEESVNNSRLHLDFAHARQKAGMATRSDILKSEVELSNAELERIKASNTLLAAQGNLN
                     QLLGMPADNDIRLVDDLSLIDKDFLPAYDSLLNEAFSLRTELKRYQSLMNAQMKYIQV
                     AKSGYYPSLSANASYNYAGPEISAMERNWWMGMTLSVPVFKGFSTKARISQEEYALKG
                     LEKDFELLKHQISQEVWNAWLAVKESYERIETSEKAFESARENLSLAEGEYKEGVGSM
                     IQLTDAQTTFVSIEQNYIQALADYKISFAELERTIGR"
     gene            7261..8313
                     /locus_tag="PLA17_04230"
     CDS             7261..8313
                     /locus_tag="PLA17_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK84654.1"
                     /translation="MKRTHLIAGIILIFITFILLWLFLFRQDNKSNTELQTVVAERRD
                     IGSSVLATGIIKPKVGAEVKVGSRISGVVKKLRANIGDYVQAGQVIAEIDDAELRAKL
                     NQNLAAVNKARADYDYAKLNMERQASLLEQNYISRQTYDLAENAFKIADAQLKQAEAN
                     AEVAKVQLSYTSIYALTSGVIASVSTQEGETVMAGLAAPTFVNIIDLTRLEVQVYVDE
                     TDIGKILIGQEATFTVDTYSDTDFKGNVTAIYPKAVIQDNVVNYIVVVEIIDFKDKIL
                     RPEMTATVTIHLETRKNVIAIPSKAIIRERGERFVTVLENGTAVSRKIKTGWYNSSFT
                     EVVDGLNEGETVLLPE"
     gene            8354..9088
                     /locus_tag="PLA17_04235"
     CDS             8354..9088
                     /locus_tag="PLA17_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK84655.1"
                     /translation="MGNVIIKAVELSKIYNKNATGEIAALRNVSLEIRKGELIAIMGS
                     SGSGKSTLMNILGCLDKPTQGKLYFEGTEVGNMTDQELALLRNRKIGFVFQSFNLLAR
                     TNALENVELPLIYSDKTNISKLAREALESVGLGDRIYHYPNELSGGQQQRVAIARALV
                     NDPEIILADEPTGNLDTKSSYEIISLFQKLNNQGRTIVIVTHEQDIADHAGRIIKISD
                     GLIVSDEVNRSQKNATSELTKLLKEE"
     gene            9090..10304
                     /locus_tag="PLA17_04240"
     CDS             9090..10304
                     /locus_tag="PLA17_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK84656.1"
                     /translation="MKTRRIMINALKNLAKFKVRSFLMMIGIVIGILALTMVVSIGLG
                     AKNQVMERVKKFGLESLMIHARAGATTSQQTSSSQQVTTLTLDDAAYLKREIRAIDEV
                     APFNRKGSTITWFEKSTTAPMFGVTPAWAYVWNWDAAEGEFITEEDMNSLNRVCLLGP
                     TVKRELFGDKDPIGEIIQVGNVQFEVKGVLVSKGISPSGGDMDNRIKVPLTTFLRRVA
                     NVDYLAGIKILLKSSSDVNKTAEEIRSLLRERHNIVEGLPDDFIVTTPDEITATAEKI
                     SGTFNVFLALLAGISLITGGIVVANIMFISVNERKKEIGLRKALGANRGDILNQFLLE
                     AAAVTLLGGIIGVLLGVLGAKIMNIVMETPVSVSWASILTGVIFSTIIGIIAGIQPAR
                     RAAGLQPVDALR"
     gene            10349..11572
                     /locus_tag="PLA17_04245"
     CDS             10349..11572
                     /locus_tag="PLA17_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK84657.1"
                     /translation="MKITKSIRISRNQLLAHKLRTALALLGIIIGVAAVIIVVAIGNG
                     AQKEVLSLIESMGTDLIIVNAGQVRKTAGRQQVSGTATTLTIRDSEFIADECPDVKYA
                     VPVQTKSMQVKFGNLSTNTTITGTTSDFERVRNFRAETGSFFTEEDNRASMRYAVMGR
                     TVVKNLFGESDPLGETIRIGKVTFIVTGVMESKGVDMNGADQDDQIFIPLQTALRRVF
                     NLTYINTIYVQARSSEKMQNAVDQVSELLRERHRLNRLQKPDDFTIQTQVELLEAQKE
                     TTDTFTSLIVGIASISLLVGGIGILAIMLIAIRERINEIGLRMAVGAGKIDILVQFII
                     ESSILSISGGVIGILIGIVVSVIVSLATKWNLIISVPSIFYSFLFSIVVGMFFGVYPA
                     RKASLLDPIEALRNE"
     gene            complement(11588..12751)
                     /locus_tag="PLA17_04250"
     CDS             complement(11588..12751)
                     /locus_tag="PLA17_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPK84658.1"
                     /translation="MSKLIYLDNSATSFPKPDVVYDFMHSFYKKHGVNPGRSGFDAAL
                     ETEEVVANTRKMLTNLFNGGGDHNRLTFSYNATDSLNLVLQGLANSGDHVITTMLEHN
                     SVLRPLYHMQEEGRIDVTHIPFNSDGFVDPDDIKKAVRKNTRMVVVNHCSNVIGTVQP
                     VSEIGKICREAGLLFVVDGSQSAGAIPVDMQAMNIDAFIFTGHKCLMGPTGIGGSYVR
                     EGVEIKHTRYGGTGVRSAYPRHLDEYPYRLETGTLNLVGVAGLNAGVKWVTEQGMENL
                     HRKEMALWSRLKAGIEKNENVITYCAGSAENHNPVLSFNIEGFEAGDVGTMLDVDYNI
                     ACRTGLQCAPLVHRHLGTEEIHGTVRLSIGPFTTEEDVDAAIEAVADIASLRR"
     gene            complement(12786..14066)
                     /locus_tag="PLA17_04255"
     CDS             complement(12786..14066)
                     /locus_tag="PLA17_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPK84659.1"
                     /translation="MTAEQKRQKALEVISRKDNGRLLTYLNSCVHCGLCAESCIFYLT
                     TKDPKYIPARKVDLVASVYRRYNTLTGKLVPGLTGARELDGDTIKEMIDILYGACTMC
                     GRCTIHCSIGIDIADVVRAGRSMLATMDLVPATLQSTVMAAIETGNNMAIPTEDLVDT
                     IKWLEEDLQFELDDQNAAIPLDEPGKKIIYTLNPREPKFFPLSISAMAKVFYMAGESW
                     TLSTEMYDVTNYAYFSGDDTLAAEIVNRLEEVTLKLGAGKVVLAECGHGSRAFRWEGP
                     NYIHRRYLFEVVTSIELLKEYIDTGRIKPDKSKNKRRVTLHDPCNLVRGGGIMKQQRD
                     ILNSCVTDFVEMTPCGNDNYCCGGGGGQLSMSEYNERRMSAGRIKADQIRATGAEIVV
                     TPCHNCVDQLIQINQAYKLNVQIKTMAEIVADAL"
     gene            complement(14080..14700)
                     /locus_tag="PLA17_04260"
     CDS             complement(14080..14700)
                     /locus_tag="PLA17_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84660.1"
                     /translation="MEWFRILALISAAVCLTALLSHFIRIVRAGKPVDYALRAGDTTR
                     GIIYSISGAMSPTKKESAYLHLPTYTAGIIYHLGTFLSLLLFIILLAGFTPSGTLSLV
                     ISLLLAVSTLAGLMILVKRFLNNELRSLSNPDDYISNLLVTLFHLMTIAALNFEGARI
                     FYFLEASLLMLYLPVGKLRHTIYFFAARYHLGYFFGYRGTWPPVKQ"
     gene            complement(14712..15029)
                     /locus_tag="PLA17_04265"
     CDS             complement(14712..15029)
                     /locus_tag="PLA17_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPK84661.1"
                     /translation="MPQIEIEGRVFEVDGDGFLVDPDLWNDEVAELFAKSDGIEEMTD
                     KHWAVVRVIRKHFEENGNAPMVRVLCRDSGVRLKEVYELFPLGPARGACRIAGLPKPD
                     GCV"
BASE COUNT         4406 a         3228 c         3534 g         4274 t
ORIGIN      
        1 attatcattt atgaattctt caaaatcttc ctttttacat gagtctgcaa gggaacatcc
       61 ggccataaga tccggtatta tcaccttttt ttcaggcgaa agtattttta cagtttcggc
      121 catgaattta accccggcaa gaacgataat atcagccgtt gtttcagctg ccttctgtga
      181 cagggcaaga ctgtcgccta taaagtcagc tatggcctgg atgtctgagg tctgataata
      241 gtgagcaaat attactgcat ttttctcttt tctgagagct tttattttct ctattatgtt
      301 gttatcaaca accattttat taataatatt ttttaaaatt tatttttata atgattatga
      361 tatcctgtta attctgttaa aacaatttta cgatttcatt tgcaaaaatg ggagttaaat
      421 caataataac atcctttcaa cacatcaaaa aatgtacctt ttctgaaata tagacaatta
      481 gagatgtaat taacaattgt tacacttgtt ttatcaatag atgcgaaaca ggtaaatcaa
      541 tggcggaaac tgtttggtaa acgttgaaag taatttgtca attaccagga aaaacatttt
      601 gatttgattg taaaaaaaca tggtgttgac aaccacgggt tatgatttat tgaatgaaca
      661 aataatcaaa tcccgggtta catgttatga acaaatcaaa cttccgtgtt ttcttttctt
      721 tttataatta cagatttata tttgtatgaa aatataaaat caatttggaa aaaattataa
      781 ttgcctcaga ccacgcgggc ttcaggctga aggagaagct cgtgagctgg ctcatcacca
      841 gcaggtacga agtcagggat attggatgtt tttcagagga tagtgttgat tatcctgatt
      901 atgcacatcc gcttgccgtt gcggtagaga atggtgaata tgattatgga ataaccattt
      961 gcggatcagg aaacgggata agcatggctt gcaacaagca cgcctcgatc aggtcagcgg
     1021 tttgctggaa taatgagata agcaggttgg caaggtcaca taatgatgcc aatatatgtg
     1081 ctcttcctgg aagatttctc accgaagcag aagcaataca gattgtaaaa gtatttctta
     1141 ccacctcttt tgagggtggc aggcataaga gacgcattga caagattcca atcaaaactg
     1201 tgtaaatgct gtcagccacc tgtaaatatg gcatcagggc agtgttatac attggctcca
     1261 aaccagatac aaaggttaat gttggtctta aggagatatc tgagaatttg aaaattcccc
     1321 agccttatct tgcgaagata ctgcaggtgc ttgcccgcaa aaagattctt cggtctacca
     1381 agggccccca cggagggttt tatctgttgg ttccggcaga gaaactgaca ctgatggata
     1441 ttattgatgc cattgacgga agggattttt ttgacagttg ctatgtgaca ggggagaggt
     1501 gcaattttga taagcccgat gatggcaggt gcattctcca taatgatctc agggaagaga
     1561 agatgcgtct tgaaaaattc tttagttcaa aaactgttga tcagataatt gccagggtca
     1621 ggaatgatcc gtctgtgaag atctgaatct ctcaaggatt ttaagtgcta tatgtgccag
     1681 caggatgcct gccactatac ctcctatgtt ggcagccata tccatgcact cagccttgcg
     1741 tgttgtggtg gtcatctgga gtatctccat cagagcaccg aaaaacaggg gtataagcaa
     1801 aataatatat cttaagttac ggcttgtttg ccagcggcat gaatcaagaa gaagtatagc
     1861 cgagaaaaac atgtacatta caaaatgccc cattttgtca agatagggta tgtttagcat
     1921 ccgccccggt atcagggact tgcttccgga gagactgagc cacagaatta acaaccctat
     1981 tattgtactc ttataatatt gttttataaa gagatatacc atgattgctt ttgaaacgtc
     2041 gaaaataaca aactagtatt aattcttttg taattttaaa gtataaattt tatcaatggc
     2101 gggaatttac atacacatac cattctgcaa aagtttctgc aattactgtg atttttacag
     2161 tataacagat gacagtctta agggtgattt tgtcagggcg cttatacgtg aagcctccct
     2221 gagatcatcc tatctggaag gagaaaccgt ggagaccata tacttcggcg gcggcacacc
     2281 atcactgctt gagcctgcta cagcagcagc cattattaat gatctgagaa aaatatttcc
     2341 tttttccggt gatccggaaa taactttcga agtcaaccct gatgatgttt ctgaaggcat
     2401 gcccggtgaa ctgaagaaag ccggcgtaaa caggataagc cttggagtgc agtcgtggga
     2461 tgacaggagc cttcgttacc tgggccgcag gcatgatgca gcccaatcgg tcagggccct
     2521 cgaaatgatt ttcggggaag gaatcaataa tgtctccgtt gacattatat acggcgttcc
     2581 cggaacaaca accgttgatc tgaaagctga cctggaaaag acccttgttt ttcccgttac
     2641 tcatctgtcg gcttatcatc ttactgtgga ggatggcacc aggtttggcc ggatgaagaa
     2701 ggaggggaag ctgaaagaga ttgatgagga gagttcttct gccatgtatt ctttgcttgt
     2761 cagatactgc agggagcacg gcttcgtcaa ttatgagatc tcaaattttg cgaaggaggg
     2821 gtttatatca aggcataact cttcatactg gaagcagata ccctatctgg gtcttggtcc
     2881 ttcggcgcac tcgtttgaca ggcgttcacg ccagtggaat gtctccgatg tgaggaaata
     2941 cattagggct gtcagcagcg gcgagatccc ctgcgagagg gaggagcttg acaagataat
     3001 gatgttcaat gagtatgtaa tgacaacgct gagaacgatg tggggtattg atcttatcca
     3061 tgtggaggag ttctacgaca aggagttgca tgattacctg gtcaatctct cgggcaaata
     3121 tatcacctat ggcctgatga aaagggaaaa aaataccctg gtccttacag accagggcag
     3181 aatgatgtct gataatatta ttgccgaatt gctggcagat ctgtgatcag ccttttccct
     3241 tcagtgagaa ggctccggtt ccgatttttt cgccgtcaag atatagctcg acatcgtagg
     3301 ttcctgttat aaagtcgcct gtattgtcga ggaaaataca catctcgatg tcagtgtttg
     3361 cataatcaac ggccctgttg gctgagaaga tcagctgttc gcccttgacc tcgaagagat
     3421 tgtcaggtga aggagtaata accagctggt cggggcgcac cactcgcagg taaacaacct
     3481 tattgcctgc tgaagcaatc gggttttccc tcagggtgaa gcagacgcgt accttgtcga
     3541 ggtttctcaa ctcagtggtc tccttgcgtt tcttgttgag ggctactgca accacatctt
     3601 ttgcctggat catcgaggca accgtgactt tggatgtcag ctcttccttc actttttcaa
     3661 gttcgacatt ggtctcctga accgcagtca tctgttgttt gatctcaacg ttttctgcag
     3721 tgagcatctg gttgcgcgtg ttcagtgagt ctatctgaac aatataactc ttcattattt
     3781 cacgcatggt gctgatctca gccttgtatt tcctgatgag ctgggcattt gaagcgttga
     3841 cctcaagaag cttctgaatc cttgtccgct cgcgcaggag ctcagcattc agcgtgtcat
     3901 tggatgtctt aagggtatca tagccataca tcatcatgcg cagctcattt gccagagagt
     3961 ctttctcttc tgtaagcacc tgctccatct caaccatttt actcttctgg ttatagtaca
     4021 ggaccaccag ggcaattaag cccagggtca gaattatggt tgaaacaatc atgacaaccg
     4081 gagcgccttt cttgtgctca acgtgttctg cctgatattt ttcctgattt tccatttgtt
     4141 tgtattttaa tccggtgtaa aattaacaat tattacagta caacaaattt ttctcatgtt
     4201 tgattatctt cgccttacat ttatataaaa gtcacactta ttttttctca tgagcggatt
     4261 ttacggttct gtctccaaaa ggagttgtat tgatgatgtt ttttatggta cggactatca
     4321 ttcacacctg ggtaccaaga gggccggatt ggttttctat gacagggaga taggattcag
     4381 gaggcgaatc cacaaccttg aaaatgagta ttttcgtagt aaattcgaag cagagctgaa
     4441 ggaatttaaa gggaacagcg ggatggggat aatcagtgac actgatccgc aaccgatact
     4501 gtttgactca cacctcggcc gttttgccct ggtgactgtt tcgaagattg tgaacataaa
     4561 tgaactcgct gattatttcc tggcacgcaa gaagcatttt accgaaaatt tcatgggaaa
     4621 catcaacccc acagagatgg ttgccaatct tatctgtgaa ggagattcct atgtagaggg
     4681 gatcgaaaat gtatttgaca agatacaggg atcatgttca ttgctgttgc tgaccactga
     4741 cggcatcatt gcagcgcgcg acaagctcgg aaggacgccg gtggttattg gcatgaagga
     4801 gggggcccgg gctgttgtat cagagacctg ttctctgccg aataccggtt ttgaaactga
     4861 atattatctc gggcccggag agattgtgca catacgtgcg agcgggttta cgaggctcaa
     4921 ggcacccgga agacggatgc agatttgctc ctttttgtgg gtttattacg gattccccac
     4981 ttctgaatat gaaggaagga atgttgagca gatacgttat ggttgcggcg ctgcgcttgc
     5041 caggcgtgat ccggtgaagg ctgacctggt gtgcggtgtc cccgactctg gtgttggtca
     5101 tgcgattggt tattcaaatg agaaacagct gccatatatg agtgtctttt caaaatacac
     5161 tcccacctgg ccgaggagtt tcacgccgca gaggcaggtg cagcgtgagc ttgtggccaa
     5221 gatgaagctc attccaaaca aaaagcttat caagggcagg aagataatat ttcttgatga
     5281 cagcattgtc aggggaacac agatgaggaa caacacccgt gatctgcgcg atgccggcgc
     5341 cgctgagctg cacatgcgca tagcatgccc gccgctcctg tatccatgcc agttcctcaa
     5401 tttttcacag tcgcgatcaa ccctcgagct tgctgcccgc aaggctatta tcaggcttga
     5461 gggagatgaa aagaaggttg aagaatatct tgatcctgac tcagagaagt acgggaaaat
     5521 ggtaaaagag attgccaggg atctcggaat ggattcactt atgtttcaac gacttgatga
     5581 tctggtaaaa gccatcggtc ttccccggga aaagctgtgt acccattgct gggacggcag
     5641 cggcaaattc tgaatttcag tcagttttta tttctggttt taattctatg gtttaacagg
     5701 gtcaaataag tatccattgc gtttactttt tttggtattg cgattaaacc tattgaacca
     5761 atccgcatct gagtaatgat aaactatgat caatcatagg atgcaattac tcatgaaacc
     5821 cacatctgtc aaacacataa agacctgttt atacaatttt cagttatgtg ggctccatca
     5881 tatttttaaa atcaaaacta catcatgaca agaatttgcc tgaaaaagag tggcatgcaa
     5941 ctgttatttg agatgaaaat tcccggaata acagacctga aacctctctc tctttttttt
     6001 atatacctac tgttattaag tgaaatcaac ttgtctgctc aggatactgt ttcagtccgc
     6061 ttgaatctga aagagtgtat tgaattcgga cttaaaaaca atcccggact tcaatcttca
     6121 atatatcttg ttgaagaaat gaaagcaaaa acagatgaag cattatccgg atattaccct
     6181 gctgtaagtt tgaattcgga tgcagataca tattcaaaga acaatggcag tcagcgctat
     6241 aataatatta gttctggaat taatttctca tataatattt ttcagggaaa caggataaag
     6301 gcaacatatg gtgcctcgca ggacaattac caggcaagca gatataatca tgaaactctg
     6361 aggcaagatc ttgtatttaa tattataaag gcatattaca ggatacttca atcagaatgg
     6421 atactgagaa gcagtgaaga atccgtgaat aattccagat tgcatcttga ttttgcccat
     6481 gccagacaaa aagcaggaat ggcaacccgt tccgatattc ttaaatctga agttgagctt
     6541 tcaaatgcag aactggagcg gataaaagct tcaaataccc tgctggcagc tcagggcaac
     6601 ctcaatcagt tgcttggtat gccggcagat aatgatatca gattagttga tgatctctca
     6661 cttattgaca aggacttttt accggcttat gattcccttt tgaatgaagc attcagtttg
     6721 cgcactgaac taaagagata tcagtcactc atgaatgcgc aaatgaaata tatacaagtg
     6781 gcaaaaagcg gatattatcc ttcattaagc gcaaatgcca gttataacta tgccgggcct
     6841 gaaatttctg ccatggagag aaactggtgg atgggaatga ctctgtcagt accggtgttt
     6901 aaagggtttt caacaaaagc ccgtatcagt caggaggaat atgcacttaa aggtcttgaa
     6961 aaggattttg aacttttaaa acatcagata agccaggagg tctggaatgc ctggctcgca
     7021 gttaaagaat catatgaaag gatagagact tcagaaaaag catttgagag tgccagggaa
     7081 aatctttctc tggcagaagg cgaatacaag gaaggtgtcg ggtcaatgat tcagctgact
     7141 gatgcgcaga caacttttgt atccattgaa caaaactata ttcaggctct tgcggattat
     7201 aaaatctcct ttgccgaact ggaaagaact ataggcagat aacacttcaa atcataaata
     7261 atgaaacgaa ctcacttgat tgccgggata atactaatat ttataacatt cattctgttg
     7321 tggttattcc tgttcaggca ggacaacaaa agcaatacgg agttacagac tgttgttgcc
     7381 gaaaggagag acatcggttc atctgtactt gctacgggga taataaagcc caaagtaggg
     7441 gcagaggtta aggtgggttc gaggatatca ggagtggtaa aaaaacttcg ggcaaacatc
     7501 ggtgattatg ttcaggcagg gcaggtaatt gcagagatag atgatgcaga attaagagcc
     7561 aaattgaatc agaatctggc agctgtcaat aaagccaggg ctgattatga ttatgcaaag
     7621 cttaatatgg agaggcaggc atcactgctt gaacaaaact acatctcccg gcagacgtat
     7681 gacctcgcgg aaaacgcctt taaaatagct gatgcacagt taaaacaggc agaagcaaat
     7741 gctgaggtgg caaaagttca gctctcctat acttcaatct atgccctgac atcaggggta
     7801 attgcatcgg tctccacaca ggagggggaa accgtaatgg caggtcttgc tgccccgact
     7861 ttcgtcaata taattgacct cacccgcctt gaggtacagg tttatgttga tgagaccgac
     7921 ataggtaaaa tcctgatcgg acaggaagcc acatttactg tcgataccta ctcagatact
     7981 gattttaaag gaaatgttac ggcaatatat cctaaagctg ttatccagga caatgtggta
     8041 aactatatag ttgttgttga aataattgat tttaaggaca aaattctcag gcctgagatg
     8101 acagctactg ttacaattca tctggaaacc agaaagaatg taattgctat tccgtcaaag
     8161 gcgataatca gggaaagggg agaacggttt gtaacagtat tggagaatgg cacggcggta
     8221 tcaagaaaaa taaagacagg ctggtataat agtagtttta ccgaggtggt tgacggattg
     8281 aacgaaggag aaacagtgct gttgcctgaa taagcaatga caagaccaga gaatatacta
     8341 aatggaaaac attatgggaa atgtaattat caaagccgtt gagcttagta agatttacaa
     8401 caaaaacgca actggtgaaa ttgccgccct gcgcaatgtc tcccttgaga tcagaaaggg
     8461 agagctgatt gcaataatgg gctcttccgg ttcaggaaaa tcaacgctga tgaatatcct
     8521 gggctgcctc gacaagccaa cacagggtaa actctatttt gaaggcactg aggttggaaa
     8581 catgacagat caggaactgg ctcttctcag aaacagaaag atcgggttcg tattccagtc
     8641 ctttaacctg cttgcaagaa caaatgcact tgaaaatgta gagctacctc tcatctattc
     8701 tgacaaaaca aatatttcaa aactggccag ggaggcactc gagtctgtcg gacttggtga
     8761 cagaatatac cattatccca atgagttatc cggcggacag caacagcgtg tggcaattgc
     8821 ccgggctctg gtaaatgacc ctgaaataat tcttgcggac gaaccgacgg ggaatctgga
     8881 cacgaaatca agttatgaaa tcatttctct ttttcagaaa ctgaataatc agggcaggac
     8941 aatagttatt gttacacatg aacaggatat agctgatcat gccggaagga ttatcaagat
     9001 ctctgatggc ttaatagtca gtgatgaggt gaacagatct cagaaaaatg ccacatcaga
     9061 actcacaaaa ttattaaagg aggagtagaa tgaaaaccag gagaataatg ataaatgcac
     9121 tgaagaattt ggcaaagttc aaggtgcgct ctttcctgat gatgatcggg attgttatag
     9181 gaatacttgc tcttacaatg gttgtatcta ttggattggg agccaaaaac caggtaatgg
     9241 aacgggttaa gaaattcgga cttgaaagcc tgatgataca tgccagggcc ggagctacaa
     9301 cgtcacaaca gacttcgtcg agtcaacagg ttaccactct aacccttgat gatgctgcat
     9361 atctcaagcg tgagatacgc gccattgatg aagttgcccc atttaaccgc aaggggtcaa
     9421 ctataacctg gtttgaaaaa tcaacaactg ctcctatgtt tggggtaact cctgcatggg
     9481 catatgtctg gaactgggat gctgctgagg gagaatttat taccgaagag gacatgaatt
     9541 ccctgaacag ggtatgtctg ctcggtccta cagttaagag agagttattt ggtgataaag
     9601 atcctatagg agagataatt caggttggca atgtgcagtt tgaagtcaag ggagtactgg
     9661 tttcaaaagg tatcagtccg tcaggtggag atatggataa cagaatcaag gttccgctaa
     9721 caacttttct gagacgtgtg gcaaatgttg attacctggc aggaatcaag attctcctga
     9781 aaagttcatc agatgtaaat aaaacagctg aagagatcag atcacttctc agggaacgtc
     9841 ataatattgt tgaggggtta cctgatgatt ttatagttac aacaccggat gagataactg
     9901 caacagcaga gaaaatatca ggtacattca atgtttttct cgccttactg gccggaatct
     9961 cactgataac cggaggtatt gttgttgcca acataatgtt catatcggta aatgagcgga
    10021 aaaaagagat tggtttaaga aaagcattgg gagcaaacag aggtgatatt ctgaatcagt
    10081 ttcttttgga agcagcagcc gttaccctgc tgggagggat cataggtgtt ttgctgggcg
    10141 tactcggagc caaaatcatg aatattgtaa tggagacgcc tgtctctgtt tcctgggcaa
    10201 gcatactcac cggagtaatc ttctctacaa taataggcat aattgcagga attcagccgg
    10261 cccgcagggc tgccgggctt cagcccgttg atgccctcag ataagaacca attgaaaagg
    10321 agtatccgga tgttcctttt ttataatcat gaagattaca aagagcataa gaatttcgcg
    10381 aaatcagctt cttgcacata agctgcggac agctcttgcc ctgcttggaa taataatagg
    10441 agtagcggca gtaattatcg tggttgcaat tggtaacggt gcacaaaaag aggtcctttc
    10501 acttattgaa tcaatgggaa ccgacctcat catcgttaat gccggacagg tgcggaaaac
    10561 tgcaggacgg caacaggtat cgggcactgc tactaccctg acaatcagag attcagaatt
    10621 tatagctgat gaatgtcctg acgtaaaata tgctgttccg gttcagacaa agagtatgca
    10681 ggttaaattt gggaacctta gcactaatac aactattacg ggtacaactt ctgattttga
    10741 gagagtcagg aatttccgtg ctgaaacggg atcatttttt acggaagagg ataacagggc
    10801 atcgatgcga tatgctgtta tgggccggac agttgtaaaa aacctttttg gggagagtga
    10861 tcctcttggt gagaccatcc gtataggaaa agtaacattt attgttaccg gggtaatgga
    10921 atcaaaaggg gtagatatga acggggcaga tcaggatgac cagatcttca tacccctgca
    10981 gactgccctg cgaagagttt ttaacctgac atatataaat acaatttacg ttcaggccag
    11041 atcgtctgaa aaaatgcaga atgcagttga tcaggtatca gaactattgc gtgagagaca
    11101 cagacttaac aggctgcaaa aacctgacga tttcacaatt cagacacagg ttgaattact
    11161 tgaggcacag aaagagacca cagatacttt tacatcactg atcgtgggta ttgccagtat
    11221 ttcactgctt gtcggaggca tcgggatatt ggcaattatg ttgattgcca taagggaaag
    11281 gatcaatgag ataggtttaa ggatggctgt gggagcgggt aaaattgata ttctcgtgca
    11341 gtttataatt gaatcttcaa tcctcagcat aagcggagga gtaataggaa tcctgatagg
    11401 aatagtggta tcagtaatag tcagtctggc aacgaagtgg aatctgatta tttcagttcc
    11461 gtcaatattc tattcatttc tcttttcaat agtggtaggg atgttttttg gcgtctatcc
    11521 ggcaagaaaa gcatccctgc ttgatcctat tgaagcattg agaaatgaat aaccgggtag
    11581 ctgacagcta ccttctcagt gatgcaatat ctgcgacagc ctctatggct gcgtcaacgt
    11641 cttcttcagt agtaaatgga cctatactga gtctgacagt gccatgtatc tcctctgtgc
    11701 ccaggtgcct gtgaaccagg ggggcacatt gtaaaccggt acggcatgca atgttatagt
    11761 caacgtcaag catggtaccg acatcacccg cttcaaatcc ttcgatgttg aaactcagaa
    11821 cagggttgtg gttttctgca cttcctgcac agtaggttat cacattttca ttcttctcaa
    11881 taccggcttt cagcctgctc caaagagcca tctccttgcg gtgaaggttt tccataccct
    11941 gttcggtcac ccatttgacg cctgcattca gccctgccac accaacaaga ttaagtgtac
    12001 ccgtctcaag ccggtatggg tattcatcga ggtgccgggg gtaagctgaa cgtacacctg
    12061 tgcctccgta acgggtgtgt tttatctcaa caccttccct gacatatgag ccaccaattc
    12121 cggttggccc cataaggcac ttgtgaccgg tgaagatgaa agcatcgata ttcatggcct
    12181 gcatgtcaac gggaatcgct cctgcgctct ggctgccgtc aaccacaaat aacaatccgg
    12241 cctcccggca gattttacct atttccgata ccggctgcac agtacctatc acattcgaac
    12301 agtgattgac aacaaccatc ctggtgtttt tcctgacagc ctttttgata tcgtcgggat
    12361 caacaaaccc gtcactgttg aaaggaatat gggttacatc aatccttcct tcctcctgca
    12421 tatggtacaa gggcctcaac actgaattgt gttccagcat ggtggtgatt acatgatcgc
    12481 cgctgttggc cagtccctga agcacgaggt ttagcgaatc ggttgcatta tagctgaaag
    12541 taagtctgtt atgatctcca ccgccattga agagatttgt caacatcttc ctggtgttcg
    12601 caacaacctc ttctgtttca agggctgcgt cgaagcccga ccggcccgga ttcacgccat
    12661 gctttttgta aaaactgtgc atgaagtcat aaacaacatc aggtttcgga aaactggtgg
    12721 ctgaattgtc aagatagata agtttgctca tcgggttgta ttttgaaagt tttcgcgata
    12781 tgcatttata gcgcgtcagc aactatttct gccatcgtct ttatctgtac attgagttta
    12841 tatgcctggt taatctgtat aagctggtcc acgcagttat ggcagggagt aacaactatc
    12901 tcggcgcccg ttgcccttat ctggtcggcc tttatcctgc cggcagacat gcgcctttca
    12961 ttatactcgc tcatcgacag ttgtccccct ccgcctccgc aacagtagtt gtcgttacca
    13021 cagggagtca tctcaacgaa gtcagtgaca cagctgttga ggatatccct ctgctgcttc
    13081 attataccgc ctcctctcac caggttgcat ggatcgtgaa gggtgaccct tcgtttgttt
    13141 tttgacttgt caggcttgat acgtccggta tcaatatatt cctttagcag ctcaatggaa
    13201 gtaaccacct caaagaggta tctccggtga atgtagttgg gcccttccca tctgaatgcc
    13261 cttgaaccgt ggccacactc cgcaagcact accttcccgg cgccaagctt caaagtcacc
    13321 tcctcaagcc ggttgacaat ttcagcggca agggtgtcat ctcctgagaa gtatgcatag
    13381 tttgtaacgt catacatctc tgtagacaat gtccagcttt cgccagccat atagaatact
    13441 tttgccattg ccgaaattga aagcggaaag aatttcggtt ctcttggatt cagtgtataa
    13501 ataatctttt tccccggttc atcaagagga attgctgcat tctgatcatc gagctcaaac
    13561 tgcagatcct cctccagcca cttgattgtg tcaacaaggt cttccgtcgg tatggccata
    13621 ttgttcccgg tttcaatggc tgccattacg gtactctgaa gagtagccgg aacaaggtcc
    13681 atcgtggcaa gcattgaccg gcctgcccgt acaacatcag ctatgtcaat gccaattgaa
    13741 cagtgaattg tgcatcggcc gcacattgtg caggcgccat acagaatgtc aatcatctct
    13801 tttattgtat ccccgtcaag ctccctcgct cctgtcagtc cgggaaccag cttgccggtc
    13861 agagtgttgt atctcctgta aacagaagct accagatcaa ccttccgtgc cggtatgtat
    13921 ttcggatcct tggtggtaag gtagaagata cagctctcgg cgcatagccc gcagtggaca
    13981 caactgttca ggtatgtgag aagcctgccg ttgtctttgc ggcttattac ctcaagcgct
    14041 ttctgtctct tctgttcagc ggtcattccc attgaagtgt tattgtttta ccggcggcca
    14101 tgttcccctg tatccaaaga aatagccaag gtgatacctt gcggcgaaga aatatatcgt
    14161 atgccggagc ttgccaaccg gcaggtaaag catcaggaga gaggcttcca ggaaatagaa
    14221 aatcctggcc ccctcaaaat taagagcagc aattgtcatg agatgaaaca gggtaaccag
    14281 caaattcgag atgtaatcat cgggattgga aagtgacctc aattcattat tcaggaacct
    14341 cttcaccagg atcattaatc cggccagggt tgacactgcc agcaggagtg atatgaccag
    14401 actcaaagtc cctgaagggg tgaaccctgc cagtaatatt ataaaaagca acagagagag
    14461 gaaggttccc aggtgatata ttattcctgc ggtataggtg ggcaggtgaa gataggccga
    14521 ttcctttttt gtcggactca ttgcccccga tatcgaatat attatacccc gtgttgtatc
    14581 tccagccctg agggcataat caacaggctt ccctgctctg acaattctga taaaatgaga
    14641 gagcaatgca gtcaggcaga ctgcggctga aatcagcgcc aaaattctga accactccat
    14701 ctgccggttt gttatacgca tccatccggt ttgggtaatc ctgcaatacg gcaggcacct
    14761 ctggccggac ccagggggaa cagttcataa acctctttaa gcctcacccc ggaatcgcgg
    14821 cataacaccc tgaccatcgg agcgttaccg ttttcctcaa aatgttttct tattactctg
    14881 acaacagccc aatgcttatc ggtcatctcc tcgattccgt cactcttcgc aaacagttcg
    14941 gctacttcat cattccacag gtcaggatct acaagaaatc cgtccccgtc aacttcaaat
    15001 actcttcctt caatttcaat ttgtggcata actaacagtt attcagctta tcagctttca
    15061 gtttctattc tatccaggat ttcatcaagt ggtgtggatg tatcaggcaa aacaaagtcg
    15121 ggacaaaagt gaagagtttt actgataatg aatctcctgc aagtctcata acccggagtg
    15181 cacccatggc accacgattc aatatatcgc tccctgctaa tttcatcaat ggggaattcc
    15241 ggatcgttca ccagtttgca tccaccaatg ttgggacaac agggatcaga cataatatta
    15301 tctcgttaaa atcatcatac agaggtgcaa attatgttaa ttaaaagaga tatcgaaatg
    15361 attattgtca actcttttct ctttttcaga caactcagat cttctgctat cactccttta
    15421 gccccataac gaaccctgga ga
//
LOCUS       DAOPBK010000047        15409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_3910_length_15409_cov_5.156962, whole genome shotgun sequence.
ACCESSION   DAOPBK010000047 DAOPBK010000000
VERSION     DAOPBK010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15409
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3910_length_15409_cov_5.156962"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLA17_04270"
     CDS             <1..491
                     /locus_tag="PLA17_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HPK84662.1"
                     /translation="GIYFFEDDYDEDTIRRNSDFYEPRCVHESSLSASIHSVIAARLH
                     DTERAYSLYLRTARLDLDDYNCEVREGLHITSMAGTWLAIVEGFGGMRIRNERVCLNP
                     VIPAAWESYSFIIRYRNNPFRVEVNRTKVIVVNLSQADIPLAVYGEQVTLKSDEERRF
                     EK"
     gene            488..2332
                     /locus_tag="PLA17_04275"
     CDS             488..2332
                     /locus_tag="PLA17_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPK84663.1"
                     /translation="MIKKVFQTLVLFMIPALLPVKGQVIERIDPPSWFTGMKNGALQL
                     MVYGREIGSYDVKTDYPGVEIMTTVRTDNPNYLFVNLNISDEAVPGTVNLIFTRGRQK
                     LAHSYPLLARPAGPARGINSSDVIYLLMPDRFANGDPSNDNVAGMTEQPDRSNPTGRH
                     GGDLKGIEDNLDYLYKLGVTGIWLNPFLENNQQHSSYHGYSTTDFYKSDPRYGSNDDF
                     KSLVTEAHKRGIKVIMDMIFNHIGSFHWWMNDLPSKDWIHQFDEFTRTNYRASTYMDP
                     YAAESDRMLMEKGWFDVTMPDLNQSNPLVETYLIQTSLWWIAFSDLDGIRMDTYSYPQ
                     PDMMSRWAKRVTDEFPGFFIVGEVWYDEPAQISYWAYNKKNSDGYRSNLPSVTDFPVC
                     FATHRAFEGKTNPTDGLSKLYNTLSQDYLYPEPLRNVIFLDNHDMTRFFTQTGQSPEA
                     FRLGLSFIMTTRGIPQIYYGTEIVMEGDKSQGDGRLRDDFPGGWPGDARNVFTGAGIT
                     SLEKEALDFTTHILNWRKNKEVIHTGKLKHYIPSDGVYVYFRYNCSESVMVIINANSK
                     ESRTISGEKYAESLEGFTFGTDIISGRKIDNLQSFTIAPQTAMIIELK"
     gene            2343..3713
                     /locus_tag="PLA17_04280"
     CDS             2343..3713
                     /locus_tag="PLA17_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPK84664.1"
                     /translation="MKEHIFKILILFIGVATLMAACRPKPAAVIEPLKTTVVHADWTR
                     NMVLYEINTRQFSEEGTFAGVQARLPQLKELGVNVLWFMPIYPIGELNRKGELGSYYS
                     ISDYKGINPEFGTIDDFKVLVDTAHAMGFHVILDWVGNHSAWDNPLATEFPEWYEKDS
                     LGNFVSPFDWTDVIQFDYTAEPLWDYMLSAMRYWVEEVGIDGYRCDFPGLVPEEFWFR
                     ATTELNSIRPVLMLAEDEEHSYLLERAFDMNYGWAHHHLMNAVAGGQRKPAALDSIIQ
                     YELKRYAPESYRLRFMTNHDENSWNGTIKEKMGEAQKAFACYLFTIPGVPLLYNGQEA
                     DLDKRLEFFRRDPIEWKETELTPFYTKLVHLRTSHPALRHGTEGGSYDVLKTSSRNVV
                     AYKRVRGENEVLTILNISNKPVTVALKDGATAGTYRDLFTDGSMEITRGGKMQLGPWG
                     YMVLVK"
     gene            complement(3930..6749)
                     /locus_tag="PLA17_04285"
     CDS             complement(3930..6749)
                     /locus_tag="PLA17_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPK84665.1"
                     /translation="MKIKIPALVLIFAGWNLAASAQVVTTDPVFPVASGPVVITFHAD
                     KGDMGMKDYTGDDVYAHTGLITSLSSGPSDWKYVVSGTWSTTVPPPDKVKLTRVGTNT
                     YTLTISPSIRAYYGVPESETIQKLAFVFRNGNASRTGRDVNGADIFYDVTEESSYEIL
                     LSEPANYTSIVNAGDVVPVTFSASVSDSLILYQNGVQLKKVTETTLTHSVTAAGSGSY
                     KLLAKAWYNNVMKADSAYYFIRTPTVVTPVPAGLKPGVNITGDNSAAFLLYAPDKSSV
                     FVIGDFNSWVYSSEGFMKRSPDGNWYWLEVTGLDPDGEYGFQYLVDETLRIPDPYTTK
                     VLDPWNDKYIDESVYPELKPYPEGVTSELVSVFRTRPPEYTWKNNDFTPPARDTLIIY
                     EMLVRDFVETHTFRSVRDTLDYLKRLGVNAIELMPVNEFEGNSSWGYNPSMYFAVDKY
                     YGTPDDLKELIDSCHGRGIAVIMDIVLNHSYNSNPLVRLYWNSANNQPAANNPWFNAT
                     TPHYYSFGGSDFNHESNDTKALIDSICHYWIDEFRFDGFRFDFTKGFTNKISNNDYSV
                     SAYDPSRIAILKRIADRIRAYKPDALLILEHFTDNAEERELAAYDFLLWGNGKWRYAD
                     ASTGNSSDLSDASWKLLGFDRPGVIDYMESHDQERIMYLNLTRGKADGGYNIKDFNTA
                     IKRVKLTATFFLTIPGPKMLWQFQELGYDVILGDGYQRLEEKPILWNYYTVPVRKNLF
                     DNFAALIELRKKYPAFSSDNFTLFQSGMTKRLNIVHPDMDVVILGNFDFFPRTIDPNF
                     TRTGPWYEFFRGTQIEVNASNQNTPLSLVQGEYRIYTSKQVDRPSFLLGMEEPLADND
                     EGPVFDVGPNPFRERTVIKFTGDDQYKPHTVEVISANGAVLQIMSTPAGIDNVILDGS
                     AFDAGLYYLKVTTGRVSAVKRVVKY"
     gene            complement(6871..8421)
                     /locus_tag="PLA17_04290"
     CDS             complement(6871..8421)
                     /locus_tag="PLA17_04290"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HPK84666.1"
                     /translation="MKSILKIMMLPVTAVLLLAGCTQETSEIRLDPKLGTTKVFNITS
                     NSATVTGFVVAEGDGFTMRGICYDKQPAPTTSGPTAQYTGAVTNAAYDVTLTGLDFAR
                     TYYARAFATGPAGTVYGEELTFTTLPIIPTVTTAEATDVEGTTAVSGGSVTADGGSAV
                     TARGVCFGTSTRPTIDAPNGKTVDGEGLGDFVSNLTGLKGLTTYYARAYATNSAGTAY
                     GNEITFTTLVSVREWYVPGDYVGASYPNSGMADWSPDKSPMVKSNEANPNNVEGYIYM
                     ANGSNWFKFATQPNWDGPNYGVDPTPGVLNPAGDNLNLPAGYYKININAGVEPMTYTA
                     VAMNWGVIGDLNGWSAQVPLTYDRNVRVWKGGMHMPAGGWKFRANDDWAYNYGAPAGS
                     ENLVAGGDNIATAVEDDYAITLDLSTPLAYTYRADRWGLIGDATPGGWDNDTNMTWDA
                     VNGCFTVTLNLTANSFKFRANDGWDYNLGGSLTALTPGGDNISVTEAGNYTITLDPWT
                     KVATMTRN"
     gene            complement(8457..9488)
                     /locus_tag="PLA17_04295"
     CDS             complement(8457..9488)
                     /locus_tag="PLA17_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HPK84667.1"
                     /translation="MKKNILIYLTFIGLAALITSCEKDGEKVIMLSNPTAPALTTIPD
                     LTFQRVNGMNVLEFVGTPVNPGFQASATYYLEACASGTSFADPVTLWTGSQCKSMKAT
                     VSDLNGILIKKFPADASSALDLRLRAVLVVDAGTGAPGTGTNTFTYNSDLKSVTVTLY
                     GLPRLDLIGSGMTQKIESTLGDGKYTGLVKLNPANAFTLTDPDAGTNYGGAGGVLAVN
                     GAAIVPENAGWNRLNVDVNALTYDISEYRIGLVGSATPNGWDTPDQPMEYDPATGTWS
                     ITIDLIEGEIKFRLNNGWAWNLGGTPDNLTHDGPNIPLTAGNYTITLTITQPNGPNEA
                     GSCIIVKNN"
     gene            complement(9507..11135)
                     /locus_tag="PLA17_04300"
     CDS             complement(9507..11135)
                     /locus_tag="PLA17_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK84668.1"
                     /translation="MNKTILIFGIILSALMISSCVKDLDVAPQDPTKILAGNLGDDPA
                     YMDQALAKIYASFIISGQGANGGDDIAASDGNFFTTMRALWNLQEITTDEAICAWGDV
                     GIADLNTQTWSPTNPFLTALYQRLALSITYANDFINNAQQYGTENVERYVAEARFLRA
                     LAYYWAIDLFGQFPFTTEEDGVGKFFPKIGSRQEIFAYIESELLDIENKLGAPRFSYP
                     QADQAAAWMLLSRLYLNAQVYTGTPRWNDVVTYTGKVIGAGYSLASDYRQNFSADNDG
                     RHNPEMIFAWEQDGMNVQGYVGTTFIIESSSDAQYIRAEDFHGLTSNTNWNGNRARIQ
                     FLDIMVDTIATYGNVPIPATDPTFSQCPDKRVFLKMKKDRNIPSPSASGDYGIGVYKF
                     TARNTNGSLPANYNTAFACTDFPVFRLADAYMMRAEALYRTGGDANIDLAVADINKIR
                     ERAYGNTSGNITAADLNLDFIIDERGREFYYEAQRRTDLIRFGKFTGGDYLWQWKGGA
                     YKGASTSEHLNLFPIPGDELSSNPNYNGVNNPGY"
     gene            complement(11156..14113)
                     /locus_tag="PLA17_04305"
     CDS             complement(11156..14113)
                     /locus_tag="PLA17_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK84669.1"
                     /translation="MEKLTGKRLFMLTFGIMMSLVVFGQQVRVSGTVTDAADRQTLPG
                     VNVVVKGTLTGTITGIDGEYSLMASPGDVLVFSFIGYLPQEITVGESTVINVAMRTDV
                     QALDEVVVIGYGTVKKSDATGSVSAISSKDFNKGAITSAQDLLVGKSAGVVITTSGGA
                     PGSGATIRVRGGSSLNASNDPLIIIDGVPIDNNNVSGSSNILSFVNPNDIETFTVLKD
                     ASATAIYGSRASNGVILITTKKGTAGSPLTVSYDGSVSVANAIAFVDVYSGDEIRQLA
                     LEKKELFGIDNLNLLGTQNTNWQKEIFRAAVSHDHNLSLAGSVNALPYRFSVGYTDEN
                     GILKNTDMQRFTGSLSLNPTFFNNRLKVNMNLKGMNTHHNYGDAGAIGSAVNMDPTQV
                     IYDGNVNSAGYFQWANYGANLGTANPVEQALAADNRSVVNRVIGNLQLDYALPFLPEL
                     RANLNMATDYAVSDGHNNRPRTSPSVLTSPLYGRLSDYSGTNYNNLLDFYLNYNKGLE
                     SIKSVIDVTAGYSWQHFKREGESYTRGRVDAGHPLYQMSDSSSFVTENYLVSFFGRVN
                     YTLADKYLLTFTLREDGSSRFAKANRWGTFPSVALAWKIKDESFMRNVRGVTDLKLRL
                     GWGITGQQDIGNDYPAMALYRQASEGSYYYINGKFIPTLRPDAYDPEIKWEETTTMNI
                     GLDFGFVNDRITGSIDLYKRVTDDLLNSVTIPSGSNFSNTLLTNVGSLENKGAELSLN
                     FIPVSTTDKLLSIGFNVTYNVNEITKLLITDDPNYIGILYGDAFTGQKQVTRVGYPAY
                     SFFVNRQVYDTNGNPIEGLYVDESGEGGVVNGDNADKYIYHNPVADYLLGFSARFEYK
                     NFDLSASARASIGNYVYNQVLAGASFDQMQQIGYWKNFTRALSETNFVKRQFTSDYFV
                     ENASFLKLDNVSAGYRFDNLFGTVGARVSFTVQNALILTRYSGIDPEVPGGVDNNFYP
                     RPRTFMLGIGLTY"
     gene            14340..15353
                     /locus_tag="PLA17_04310"
     CDS             14340..15353
                     /locus_tag="PLA17_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK84670.1"
                     /translation="MSRENITIKDIARELGISPSTVSRALKDHPDISQATRDAVNELA
                     ERWNYRPNPIALSLKSGSSKTIGVIIPDVVHFFFSTVISGIEDVVYQRDYNMILCQSN
                     EMWEHEVKNIKTLLSSRVDGILASVAKTTTDFGHYRSITDKDIPLVFFDRAAEEIDTD
                     SVVIDDETGAYRAVNHLLRMGKKRIVHLAGPPRLAIARNRLNGYLKAMKEYRITPQDD
                     YIVKCDDIESAERIIPELLKQTPGPDAFFAVNDLTAAQTLMIVKRHGLRIPQDIAIVG
                     FTNSQIATLTDPGLTSVDQKGFEMGQLAARLLIDRIENRHGPVQKKIITSDLVIRGSS
                     SSK"
BASE COUNT         3849 a         3797 c         3865 g         3898 t
ORIGIN      
        1 agggtatcta tttcttcgag gatgattacg acgaggatac catcaggcgc aattccgact
       61 tctatgagcc gcgttgcgtg catgaatcat cactctcggc aagcatccac tcagtgattg
      121 ctgccaggct gcatgacacg gagagggcct attcactgta cctccgcacc gcaaggcttg
      181 accttgacga ttacaactgc gaggtgaggg agggactcca cataaccagc atggccggaa
      241 catggctcgc catagtggag gggttcggcg ggatgcggat acgcaatgag cgtgtctgcc
      301 ttaatccggt tattccggct gcatgggaat catactcatt tataatcaga tacagaaaca
      361 atccattccg ggttgaggtc aacagaacaa aggtgatagt ggtgaacctc tcacaggctg
      421 atatcccact tgctgtttac ggagagcagg tcacgctgaa atcagatgaa gaaaggagat
      481 ttgaaaaatg ataaaaaagg tattccagac gcttgtttta ttcatgatcc cggcactgct
      541 gccggtgaag ggacaggtta ttgaacgcat tgacccgccc tcctggttca cagggatgaa
      601 gaatggtgct cttcagctga tggtctatgg cagggagatc ggatcatacg acgtgaaaac
      661 tgattatccg ggggtagaga tcatgactac cgtacggact gataacccga actatctctt
      721 tgtcaacctg aatatttcag atgaggctgt tccgggaacc gtgaacctga tattcacccg
      781 cggcagacag aagcttgcac acagctatcc cctcctggcc aggccggccg gaccggcaag
      841 gggaatcaac tcctcagacg tgatctacct gctgatgcct gaccgtttcg ccaacggaga
      901 cccctccaat gacaacgttg cgggtatgac agaacagcct gaccgcagca accccacagg
      961 caggcacgga ggagacctga agggaataga ggataacctc gattacctgt ataagctggg
     1021 tgttacagga atatggctga atccgttcct tgagaataac cagcagcact cctcatacca
     1081 cggctattcg actactgatt tctataaatc agatccgcgc tacggatcaa acgatgattt
     1141 caaaagcctg gtgactgagg ctcacaagag aggaataaag gttatcatgg atatgatatt
     1201 caatcatata ggatcattcc actggtggat gaatgatctc ccctcaaagg actggatcca
     1261 tcagtttgac gagtttaccc gcaccaatta cagggcttcc acctatatgg acccgtacgc
     1321 cgcagagagc gacaggatgc tgatggaaaa gggatggttc gatgtgacca tgcctgacct
     1381 caaccagagc aatccacttg tggagaccta tcttattcag acaagcctgt ggtggatcgc
     1441 cttcagtgat ctcgacggca tcagaatgga tacgtacagt tacccccagc ctgacatgat
     1501 gtcaagatgg gcaaagcggg tcaccgatga gttcccgggc ttcttcattg taggcgaagt
     1561 gtggtatgat gaaccagccc agatttctta ctgggcatat aacaaaaaaa acagcgacgg
     1621 gtacaggtca aacctgccat cggtgaccga tttccccgtc tgctttgcaa ctcacagggc
     1681 attcgaaggc aagaccaatc cgaccgacgg gctctcgaag ctatacaata ccctgtcaca
     1741 ggactacctc taccccgaac cactcaggaa tgtcatcttc cttgacaacc atgacatgac
     1801 gaggttcttc acccagaccg gccaaagccc cgaagcattc aggctgggac tgagcttcat
     1861 catgaccaca aggggaatac cccagatata ttacggaacg gagatagtca tggagggcga
     1921 caagagccag ggtgacgggc gcctgagaga tgacttcccg ggaggatggc ccggcgatgc
     1981 aagaaatgta ttcacaggcg ccggtattac ctcactggag aaggaagccc ttgattttac
     2041 aacacatatc ctgaactgga ggaagaataa ggaggtgata cataccggca aactgaaaca
     2101 ttacatacct tcagacggcg tctatgtcta tttcagatat aactgcagcg aaagcgtaat
     2161 ggtgataatc aatgcgaaca gcaaggagag ccgtaccatc agcggtgaga aatatgctga
     2221 gtcactggaa ggattcacgt tcggcactga tatcatctct ggccggaaga ttgacaatct
     2281 gcaatcattt accatagccc ctcagacagc aatgataatt gaactaaaat aacctgatca
     2341 caatgaaaga gcacattttt aaaatcttaa ttctgttcat tggtgtagca acactgatgg
     2401 ctgcctgccg gccgaaaccg gctgcggtaa ttgagcctct gaaaaccact gtcgtccatg
     2461 ccgactggac ccgcaacatg gttctgtatg agattaacac ccggcagttc tccgaagagg
     2521 gaacctttgc cggagtgcag gcgagactcc cacagctgaa ggagctggga gtgaacgttc
     2581 tgtggttcat gccaatctat cccattggcg aattgaaccg gaagggtgaa ctcggcagtt
     2641 actattcaat aagtgattac aaaggaatca atcccgagtt cggaacaata gacgacttta
     2701 aagtgcttgt tgacactgcc catgccatgg gcttccacgt gattctcgac tgggttggca
     2761 atcactcggc atgggataat cctcttgcca ctgaatttcc ggagtggtat gaaaaagact
     2821 cattgggaaa ttttgtctcg ccgtttgact ggactgatgt gatccagttt gactacactg
     2881 cagagccgtt gtgggattac atgctcagtg caatgaggta ctgggttgaa gaggttggca
     2941 ttgacggcta ccgctgtgat ttcccgggac tggttcctga ggagttctgg ttcagggcca
     3001 ctaccgaact caactcaatc aggccggtgc tgatgcttgc agaggatgag gagcattcat
     3061 acctgctgga gcgtgccttt gacatgaact acggctgggc gcatcatcac ctgatgaatg
     3121 ctgtggcggg aggccagcgc aaacctgctg cccttgacag cattattcag tatgaactga
     3181 agcgttatgc tcccgaatca tacaggctgc gcttcatgac caaccacgac gagaactcgt
     3241 ggaacggtac tatcaaagaa aagatgggtg aagcacagaa ggcattcgcc tgctatctct
     3301 tcaccattcc cggcgtgcct ctgctctaca acggccagga ggctgatctt gacaagaggc
     3361 tggagttctt caggcgtgac ccgattgagt ggaaggagac cgaacttact cccttctaca
     3421 cgaagctggt tcacctgcgt acatcacatc cggcactgcg tcatggcact gaaggcggat
     3481 catatgatgt gctgaagaca agcagcagga atgtggttgc atacaagagg gtcagaggtg
     3541 aaaatgaggt tctgactatc ctcaatatca gcaataaacc ggtgactgtg gcactgaagg
     3601 acggagccac agcaggaacc tacagggacc ttttcactga tggcagcatg gagatcaccc
     3661 ggggcgggaa gatgcagctc gggccatggg gttatatggt actggttaaa taatcataca
     3721 gacttgccac agacaaaaag ggtgtctcaa aagggcaccc tttttcaaac ccggactttg
     3781 gatccgggtt ttctcatgtc tgaaaatatt atctctgaag gcctcaggtc acggggtctg
     3841 aaggttttat ctttaaaggc aacaggcccc gggatctgaa gctcaatatg attaacggac
     3901 ttctggcgtc agaccttcag aactgtaaac tagtatttta ccactctctt tacggcagac
     3961 acccttccgg ttgtcacctt caggtagtag agacccgcgt caaatgcaga accatcgagg
     4021 atgacattat ctatgcccgc aggtgtgctc attatctgca gcacggctcc gttggcagag
     4081 atcacctcaa ccgtgtgggg tttatactga tcatcaccgg tgaattttat caccgtcctt
     4141 tcacggaaag gattcgggcc gacgtcaaac accggtcctt catcgttgtc agcaagcggt
     4201 tcctccatac caagcaggaa agagggcctg tcaacctgtt tcgaggtata gatgcggtat
     4261 tctccctgca caagggagag gggtgtattc tggttggaag cgttaacctc tatctgggtg
     4321 ccgcggaaga actcatacca tgggccggtg cgggtgaaat tcggatcgat cgtgcgggga
     4381 aaaaagtcaa aattgcccag aataacaaca tccatatcag gatgaactat gttcagcctt
     4441 ttggtcatcc cgctctggaa gagagtgaag ttatccgagg agaaggccgg atatttcttc
     4501 ctcagctcta tcagggcagc aaagttgtca aaaaggttct ttcgtaccgg tacagtgtag
     4561 taattccaca ggatgggctt ctcttccagc ctctggtaac catcaccgag gataacgtca
     4621 taccccagct cctggaactg ccagagcatc ttcgggcctg gtattgtcag gaagaatgtg
     4681 gccgtaagtt taaccctttt aatagccgtg ttgaaatctt ttatattata tcctccatca
     4741 gccttccccc gggtaagatt gagatacata atcctctcct ggtcatggct ctccatgtaa
     4801 tcaatcaccc ccggcctgtc aaaaccgaga agtttccacg atgcatcgga aagatctgaa
     4861 gagttgccgg ttgaagcatc tgcgtacctc cacttcccgt tgccccaaag gagaaaatca
     4921 tatgctgcca gttccctctc ctcggcgttg tcggtaaagt gttccagtat cagcagagca
     4981 tcaggcttgt aagccctgat cctgtctgca atacgtttga ggatggctat ccgcgagggg
     5041 tcatacgctg ataccgagta atcattgtta ctgattttgt tggtaaaccc tttggtaaag
     5101 tcgaacctga aaccgtcaaa cctgaactca tcaatccagt aatgacatat actgtcaatg
     5161 agcgcttttg tgtcattgct ctcatggttg aaatcagagc ctccgaaact gtaataatgg
     5221 ggagttgtgg cgttgaacca tggattgttg gctgcaggct gattgtttgc actgttccag
     5281 tagagcctta cgagcggatt cgagttgtac gaatgattga gaactatgtc catgattaca
     5341 gctattcccc tgccgtggca tgaatctatc agttccttca gatcatcagg agtgccgtaa
     5401 tacttgtcaa ccgcgaagta cattgaaggg ttgtaacccc agctgctgtt gccctcgaac
     5461 tcattcacgg gcatcagctc aatggcgttg actcccaggc gcttcaggta atcaagcgtg
     5521 tcacgaacac tcctgaaagt gtgtgtctcc acaaaatcac gaaccagcat ttcgtagatg
     5581 atcagagtgt ccctggcagg aggcgtaaaa tcgttgtttt tccatgtgta ctccggagga
     5641 cgtgtcctga agactgacac aagttcactt gtcactccct ccgggtaggg cttcagttca
     5701 gggtaaaccg actcgtcaat gtacttgtca ttccacgggt caagtacctt tgtggtgtac
     5761 ggatcaggta tgcggagcgt ctcatctaca aggtactgaa atccatattc cccgtcaggg
     5821 tcaagtcctg tcacctcaag ccagtaccag ttgccgtcag ggcttctctt catgaatccc
     5881 tcactgctgt atacccagct gttgaagtcg ccaatgacaa acacactgct cttgtcgggt
     5941 gcataaagaa ggaatgctgc cgaattgtct cccgtgatgt tcaccccggg cttcagccct
     6001 gccggaacgg gcgtcaccac cgttggagta cgtatgaaat agtatgcgga atcagctttc
     6061 atgacattgt tgtaccaggc ctttgccagc agcttgtagc tccctgagcc ggcagcagtg
     6121 acggaatggg tcagggttgt ctccgtcact ttcttaagct gcactccgtt ctggtagaga
     6181 atgagtgaat cagatacaga agcagaaaat gtaacaggca ccacatctcc ggcatttact
     6241 atggaagtat aattggctgg ttcggaaagc agtatttcat aactgctctc ctccgtgacg
     6301 tcatagaata tatctgcgcc gttgacatca cgaccggtcc gcgaggcgtt gccgttgcgg
     6361 aacacaaaag ccagtttctg aatcgtctcc gattccggga caccgtaata tgccctgatt
     6421 gacggggata tggttagtgt ataggtattg gtgccaaccc gtgtcagctt tactttatca
     6481 ggtgggggca cggtggtgct ccaggttccc gagacaacat acttccagtc agacgggcct
     6541 gaactcaggc tggtgatgag gccggtatgc gcatagacat catctcccgt atagtccttc
     6601 atccccatgt cacccttgtc agcatgaaag gttattacca cagggcctga tgccaccggg
     6661 aagacaggat cagtagtgac cacctgtgcc gaagcggcca ggttccaccc tgcaaatatc
     6721 agtaccagtg ccgggatctt gattttcatt gtttctgatt gatagtgtga atcttgctaa
     6781 atataatcaa aaacctcttc cagtcacaga agaggttttt gatttcaagg aacgctacgg
     6841 ccgcagccgt aacattgtga actattccgc ttagttcctg gtcatggttg ccacctttgt
     6901 ccagggatcc agggtgatgg tgtagtttcc tgcctctgtc acagaaatgt tgtcaccacc
     6961 gggggtgaga gccgtaaggc ttccgcccag gttataatcc catccgtcat tggccctgaa
     7021 cttgaatgag tttgcagtca ggttcagagt cactgtaaaa catccgttga cggcatccca
     7081 ggtcatgttg gtgtcattat cccagccgcc cggtgtggca tcgccgatga ggccccacct
     7141 gtcagccctg tatgtatatg ccagaggagt actgaggtca agggtaatgg cataatcatc
     7201 ctcaacggct gtggcaatat tatcacctcc ggctacgaga ttctcgctgc ctgccggtgc
     7261 tccgtagttg taggcccaat catcattggc cctgaacttc catccgcctg caggcatatg
     7321 cataccgcct ttccagacac gaacattccg atcatatgtc agtggcacct gggcgctcca
     7381 tccattgaga tcacctatca caccccagtt cattgccact gcggtatagg tcatcggttc
     7441 aactccggca ttaatgttga tcttatagta ccctgcgggc aggtttaggt tatcacctgc
     7501 gggattgaga acacccgggg tcgggtcaac tccataattc ggtccatccc agttgggctg
     7561 tgttgcaaac ttgaaccagt tgctgccatt ggccatatag atataccctt ccacattgtt
     7621 cggattggcc tcgttgctct tgaccatggg tgacttgtca ggcgaccagt cggccatgcc
     7681 ggagttcgga tagctggcac ctacatagtc ccccggaacg taccattcgc gaacgctcac
     7741 cagggtggtg aaggttattt cgttaccgta ggctgtacct gcactgttgg tggcatatgc
     7801 acgtgcgtag tacgtggtca gtcctttcag gccggtaagg ttgcttacaa agtcacccag
     7861 accttcacca tcaactgttt tcccgttggg ggcatcaatg gtgggcctgg ttgaagtgcc
     7921 gaagcagaca ccgcgggcgg taactgcaga ccctccgtca gcggtgacgc tgccacccga
     7981 tactgctgtg gttccttcta catcggttgc ttctgcagtt gtgactgttg gaattatcgg
     8041 cagggtggtg aatgtgagtt cctcaccata gacagtgccg gcgggtccag tggcaaatgc
     8101 acgtgcataa taggtccggg caaaatccag gcctgtcagt gttacgtcat aggcagcatt
     8161 ggtcacagca ccggtatact gagccgtcgg gcctgaagtg gtgggagcag gctgcttgtc
     8221 atagcaaatg cccctcatgg tgaatccatc gccttcagcc acgacaaatc ctgttaccgt
     8281 tgctgaattt gaagtgatat tgaacacctt ggtggttcca agcttaggat caagtcttat
     8341 ttcagatgtc tcctgtgtac aaccggcaag caaaagtacc gctgttacag ggagcatcat
     8401 tatcttcaaa attgatttca ttttcataaa tatttacttt ttataatgtc cctgaatcag
     8461 ttattcttca caataatgca tgaaccggct tcattaggac cgttgggctg cgtgatggta
     8521 agcgtaatgg tatagttacc tgctgtcaag ggaatgttag gcccgtcatg ggtcaggtta
     8581 tcgggtgtgc cgccgaggtt ccatgcccat ccattattca acctgaactt gatctctccc
     8641 tctatgaggt ctattgtaat tgaccatgtg ccggttgcag gatcatattc cattggctgg
     8701 tcaggagtat cccagccatt cggcgtagcc gaacctacca ggccaatcct gtattccgag
     8761 atgtcatagg tcagggcatt gacatcaacg ttcagtctgt tccatccggc gttctccgga
     8821 acgatggctg cgccattgac tgcaagcaca ccgccggctc cgccgtaatt cgtgccggca
     8881 tcaggatcag tgagagtgaa ggcattggcc gggttaagct tgaccagacc tgtatatttg
     8941 ccgtcaccga gggttgactc gatcttctgg gtcatgcccg atccgatgag gtcaaggcgc
     9001 ggcagaccgt acaatgttac agtaacactt ttcaaatcag agttgtaagt gaaggtattg
     9061 gttcctgtgc cgggagctcc cgtaccggcg tcaaccacca gtacagccct gagtctcagg
     9121 tcaagagcag aggatgcatc agcgggaaac tttttgataa ggataccatt caggtcactt
     9181 acggtggcct tcattgactt gcactggctc cctgtccaga gggttacagg atcagcgaaa
     9241 gatgttcctg atgcacatgc ctcaagataa taggttgcag aagcctggaa accagggtta
     9301 acaggtgtac cgacgaattc aagcacgttc attccgttaa ctctctgaaa cgtaaggtca
     9361 ggaattgtgg taagcgccgg agctgtcgga tttgaaagca tgatcacttt ctcaccgtcc
     9421 ttttcacagg aagtgatgag tgctgccaat ccgatgaatg tcagatatat taagatattc
     9481 ttcttcattt ttctttgcat taaggattaa tagcccgggt tatttacccc gttataattc
     9541 gggtttgatg aaagttcatc accgggaatc gggaacagat tgaggtgctc cgatgtgctg
     9601 gcgcccttat atgcgccacc cttccactgc cacagatagt cgcctccggt aaatttgccg
     9661 aaacgtatca ggtcagtgcg tctctgggcc tcatagtaga attcgcgtcc acgctcgtca
     9721 atgatgaagt cgaggttaag atcagcagcg gtgatattgc cgctggtgtt accataggcg
     9781 cgctccctga tcttgttgat gtcagcaacg gccaggtcaa tgttggcatc tcctcctgtt
     9841 ctgtacagag cttcagccct catcatgtag gcgtcagcga gacggaagac cgggaaatca
     9901 gtacatgcaa atgcagtgtt atagtttgca ggcagtgagc catttgtgtt acgtgcggta
     9961 aacttgtaaa cgccaatgcc atagtcgccg ctggctgaag gtgaagggat attcctgtcc
    10021 ttcttcatct tcaggaagac cctcttatca gggcactgag aaaaggtcgg atcagttgcc
    10081 ggtattggca cattgccata agtggcaatt gtatcaacca taatatcaag gaattgtatc
    10141 cttgcgcggt taccgttcca gttggtgttt gaggtcagac cgtggaaatc ttcggccctg
    10201 atatattggg catcactgga tgattcaata ataaaggtag tgccaacata cccctgtaca
    10261 ttcattccat cctgctccca tgcgaagatc atctcagggt tgtggcggcc atcattgtct
    10321 gcactgaagt tctgcctgta atcagaagcc agtgagtaac cggcgccgat aaccttgccg
    10381 gtgtaggtga ccacatcatt ccacctgggt gttcctgtgt atacctgggc attaaggtaa
    10441 agccttgaaa gaagcatcca tgctgcagcc tggtcagcct gcgggtatga aaaccttggg
    10501 gcaccaagct tgttctcaat gtcaagcagt tcagactcga tataggcgaa tatctcctgc
    10561 ctgctaccta tcttcgggaa gaacttgccc acaccatctt cttctgtggt gaacgggaac
    10621 tgtccgaaca ggtcaattgc ccagtaatag gcaagagccc tcaggaaacg agcttcagca
    10681 acataccttt caacattctc tgttccatat tgctgagcat tgttgataaa gtcattggca
    10741 taggtgatgc tcagggcaag acgctgataa agagcagtca ggaaaggatt ggtcgggctc
    10801 catgtctggg tattgaggtc agctataccg acatcacccc atgcacatat agcctcatca
    10861 gttgtaatct cctgaaggtt ccagagagcc ctcattgtag tgaagaagtt accgtcagaa
    10921 gctgctatgt catcgcctcc gttggctccc tgacccgaga ttataaagct ggcatagatc
    10981 ttagccaggg cctggtccat ataggcagga tcatcaccga ggttacctgc aaggattttt
    11041 gtcgggtcct gcggggcgac atccagatcc ttcacgcatg atgagatcat cagcgctgaa
    11101 agtataattc cgaatattaa aattgtctta ttcatatctg cttatcttta ttttattagt
    11161 aggtaagccc tataccgagc atgaaggtgc ggggcctggg gtagaagttg ttgtcaacac
    11221 cacccgggac ctccgggtca atgccgctgt acctggtaag tatcagtgca ttctgcacgg
    11281 tgaagctgac gcgggcgcca acagtaccga agaggttgtc aaacctgtaa cctgcactca
    11341 cattgtcaag cttcaggaat gaggcattct ctacaaagta atcactggta aactggcgtt
    11401 ttacgaagtt ggtctcgctg agcgccctgg tgaagttctt ccagtagcca atctgctgca
    11461 tctgatcaaa tgaagcaccg gccaatacct gattgtagac atagttgcct atgcttgccc
    11521 ttgcagatgc ggaaagatca aagttcttgt actcaaacct tgcggagaag ccaagaagat
    11581 aatctgccac aggattatga tagatgtact tgtcagcatt gtcaccgttg accacacctc
    11641 cttcacctga ttcatcaaca taaagcccct ctatggggtt gccattggta tcataaacct
    11701 ggcggttaac aaagaatgag taggcagggt agccaaccct ggtgacctgt ttctgacccg
    11761 tgaaggcatc accgtagagt atacctatgt agttgggatc atcagtgatc agcagcttag
    11821 tgatctcatt cacattgtat gtgacattga atcctattga aagaagcttg tcagtggttg
    11881 agaccgggat aaagttcagc gaaagctcag cccctttgtt ttcaagactc cccacattcg
    11941 taagcaatgt gtttgagaag ttacttccgc tggggatggt gaccgagttc agaagatcat
    12001 cggtgactct tttataaaga tcgattgagc cggtgatacg gtcattgacg aacccgaaat
    12061 cgagaccgat attcatggtt gtggtctctt cccattttat ttccgggtca taggcatcag
    12121 gcctcaatgt cgggatgaac ttcccgttaa tgtaatagta tgagccttca gatgcctggc
    12181 ggtagagagc catggcagga taatcattac cgatatcctg ctgaccggtg ataccccatc
    12241 cgagcctgag tttcaggtca gtcacgcctc tgacattcct catgaacgat tcatccttga
    12301 ttttccatgc aagagccact gacgggaagg taccccagcg gttggctttt gcgaaacgtg
    12361 atgagccgtc ttcgcgaagg gtgaatgtaa gaagatactt gtcagccagg gtgtagttaa
    12421 cccggccaaa gaatgaaacc aggtagttct cagttacgaa ggatgagctg tctgacatct
    12481 ggtagagggg atgcccggca tcaaccctgc ccctggtata tgattctcct tctctcttga
    12541 agtgctgcca cgaataaccg gctgtcacat caatgacact tttgatgctc tccagtccct
    12601 tgttgtagtt cagataaaaa tcaagaagat tattgtagtt agttccgctg tagtcagaaa
    12661 gccttccgta aagcggggag gtgagaactg atggtgacgt cctgggacga ttgttatgac
    12721 cgtcactgac agcatagtcg gttgccatgt tgaggtttgc cctcagttcc ggaaggaaag
    12781 gaagggcata atctaactga agattaccga ttaccctgtt gactactgac ctgttgtcag
    12841 ctgcaagagc ctgctcaacg gggtttgccg tgccaaggtt ggcgccatag ttggcccact
    12901 ggaagtagcc tgctgaattg acattgccgt cataaataac ctgggtcggg tccatattga
    12961 ctgctgatcc gatagctcct gcatcaccgt aattatgatg agtgttcatt cccttgaggt
    13021 tcatgttaac cttaagccgg ttattgaaga aggtgggatt aaggctcagt gacccggtga
    13081 agcgctgcat gtcagtgttc ttcaggatac cgttctcgtc agtatatccg actgagaagc
    13141 ggtagggaag agcattcact gaaccggcga ggctcagatt gtggtcatga gatacggctg
    13201 cacggaagat ctccttctgc cagttggtgt tctgggttcc gagaagatta aggttatcta
    13261 ttccaaagag ttcttttttc tccagagcca gctgccttat ctcgtcacct gagtaaacat
    13321 caacaaaggc gatagcgttg gcaaccgata cactgccgtc atacgagact gtcagggggc
    13381 taccggcagt tcctttcttg gtggttatga gaatgacgcc gtttgatgct cttgagccat
    13441 aaatggccgt tgcggaagca tccttgagca ccgtgaaggt ctcgatgtca ttcgggttta
    13501 caaacgaaag gatgtttgag cttcctgaaa cattattatt gtcgattggc acaccgtcaa
    13561 taatgatgag aggatcattt gatgcattaa gtgatgatcc cccgcgaacg cgtattgttg
    13621 cgccgcttcc cggtgctccg cctgaggtgg tgataaccac accggcactc ttgcctacaa
    13681 gcaggtcctg agctgaagtg atcgcacctt tgttgaaatc cttcgagctg atagctgaca
    13741 ccgatccggt agcatcactc ttcttgacag taccgtaacc gatgacaaca acctcatcga
    13801 gggcctgaac atcagtacgc atggccacgt tgatgacagt gctttcacct acggtaatct
    13861 cctgcggaag ataaccaatg aagctgaaca caagaacatc cccgggactt gccatcaggg
    13921 agtattcccc gtctatgccg gtaatggttc ccgtcagcgt gcccttgaca acgacgttaa
    13981 cgccgggtaa cgtctgtctg tcggctgcgt cggtaactgt acctgacact cttacctgtt
    14041 gcccgaagac aacaagactc atcattatgc cgaaggtcag catgaagagc cgttttccag
    14101 ttagtttctc cataaattga tagtttaatt acttttcaga attgttgtta tcgctagatt
    14161 aaattgtcca ggtttgggat gttaaaattc gacctttttt cagagttaac caattctttt
    14221 tcagcttttt tacgcaaacg ttgccgcaaa cgtttgaatt gagggtgagg tatgttttca
    14281 tgattaaccg ggtagcactt ttttttatta tctttagcat ctgacagctc cttactaaaa
    14341 tgagcaggga aaacatcacc ataaaagata ttgcacgcga actcggaatt tctccctcaa
    14401 ctgtttcaag ggcgctcaag gatcatcctg atatcagcca ggcaacccga gatgccgtca
    14461 atgagcttgc cgaacgctgg aactacaggc ccaaccccat agccctcagc ctgaagagcg
    14521 gatcctcaaa aactattggg gtaatcatcc ctgacgtcgt gcattttttc ttctcaacag
    14581 tcatcagtgg catcgaggat gtggtctacc agcgtgatta caacatgatt ctctgccaga
    14641 gcaacgagat gtgggagcat gaggtaaaga acatcaagac tcttctctca agccgtgttg
    14701 acggcatcct tgcatctgtt gccaagacca ctacagactt cgggcattac cggagcataa
    14761 cggataagga tattccgctt gttttctttg acagggccgc tgaagagatt gataccgaca
    14821 gtgttgtcat tgatgacgag accggagcat acagggcggt gaatcatctc ctccgcatgg
    14881 gaaagaagcg catcgtgcac cttgcaggac ctccccggct ggcaatagcc cgcaacaggc
    14941 ttaacggata tctgaaggca atgaaggagt accggatcac ccctcaggat gattacattg
    15001 tcaagtgcga tgatatcgaa agcgccgaaa ggataatccc tgaattgctg aaacaaaccc
    15061 cgggtcctga tgcttttttt gccgtaaatg acctgacagc cgcacagaca ctcatgatag
    15121 taaagagaca cggactgagg attccgcagg atattgccat agtgggattc accaacagcc
    15181 agattgcaac ccttaccgat cccggcctga catccgttga tcagaagggc tttgagatgg
    15241 gacaactggc tgcaaggctg ctgattgacc gcatcgagaa ccgccacggt cctgttcaga
    15301 aaaaaataat cacctctgac ctggtgatac ggggctcttc ctcatcaaaa taattccatt
    15361 cagataaaat cagacagcct tccccttatt cccgccctga tccctttcg
//
LOCUS       DAOPBK010000048        14916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4056_length_14916_cov_5.248436, whole genome shotgun sequence.
ACCESSION   DAOPBK010000048 DAOPBK010000000
VERSION     DAOPBK010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14916
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4056_length_14916_cov_5.248436"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PLA17_04315"
     CDS             <1..922
                     /locus_tag="PLA17_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84671.1"
                     /translation="NEIYLLQLPHGSEGWKAAGGSSGLPRPVAYGASASVRAGVVCMG
                     GENESGITDEAFMISITDGKPLIIPLPELPVPLSGAAAASSGSVVFIAGGQTPDGSSS
                     SMWSLDTDEISQGWRKHAEMPLPLINSVMAAVEGKELKIWIIGGRTRGEGDATSVIRQ
                     EIFSYSPAADRWTRAGNLTGKTGVMPLAAGTGAAIDERYVALFGGNDGSVFNKVEAVL
                     SAMATESDTLELKQLKNEYISLQESHPGFSRAVLILDTKTGRCIQAGEIPGPAQVTTT
                     AVQTPWGIVIPSGEIKPGVRTTAIRMAGFR"
     gene            997..2181
                     /locus_tag="PLA17_04320"
     CDS             997..2181
                     /locus_tag="PLA17_04320"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPK84672.1"
                     /translation="MDTQLQSIAERYRNALLNDIIPFWMKYSPDHEHGGYFTCLDRSG
                     KVYDTDKFIWLQGREIWMFSHLFNNVEKRQEWLDMALSGAAFLGKYGSDRDGNFYFSL
                     TREGKPLVQPYNIFSDCFAAMAFGELFRATGKSRYGEIALNTFRNILNRSGNPKGIWS
                     KVYPGTRPLKGFTLPMILSNLSEIMAPLLDAGTVERLRGESVHEVMDVFYSREHGLIL
                     ENVAPDGSFSDSFEGRLLNPGHAIEAMWFMMDLSEIMGDHRLAVKATEITLQTLSKGW
                     DDKHGGIFYFLDVKGYPPQQLEWDQKLWWVHIEALVALAKGYRLTGDRRCLDWFEKVD
                     AYTWSHFPDPEFGEWYGYLNRSGEVLLPLKGGKWKGCFHIPRGLLQVWKTFSDVEPVK
                     SE"
     gene            2184..3425
                     /locus_tag="PLA17_04325"
     CDS             2184..3425
                     /locus_tag="PLA17_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK84673.1"
                     /translation="MKESVAERRYAWVVVALLWVVALLNYMDRQMLSTMKNAMMVDIV
                     ELEKAENFGRLMAVFLWIYGLMSPVAGIIGDRLNRKWLIICSLFVWSGVTFAMGYTHD
                     FGVLYALRAIMGVSEALYIPAGLSLIADYHQGSTRSLAVGIHMTGLYMGQALGGFGAT
                     IADSLSWQKTFHYFGLVGIVYSLVLILFLREYKQRQIIKKASSGLSGLRSVASGLSML
                     FGMVSFWVILFYFAVPSLPGWAVKNWIPTLFSSSLGIEMPVAGPMSTITIAFSSLVGV
                     IFGGILSDRWIQKNIRGRIYTGAIGLSLTIPSLLLVGLGHTYLWIIGGAVLFGIGYGM
                     FDANNMPILCQFVTPRLRATGYGLMNMAGVFAGAFITRVLGKSMDSGNLARDMALLAI
                     PVLLAVILQLAILRPESANKE"
     gene            3496..4602
                     /locus_tag="PLA17_04330"
     CDS             3496..4602
                     /locus_tag="PLA17_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPK84674.1"
                     /translation="MILLNGEVGNYIYSNGRKYSYFAGNNYLGLAGHPEIKKAVIRAV
                     EKYGVNFAASRHTTGTSDLHLELEGALAAFKGKEDAVVFASGYQGNSILLEILKGSYS
                     TIFIDQAAHASIIAAIPADVVRVIYYDHCDAGHLDYLLDYNRGSGPLVITDGIFALTG
                     EIAPLDQIYEVVRKHNAILVVDDAHATGVIGATGKGTPEHFGLPGDGRIYQTETMSKA
                     LGGYGGFISGSRQVTGMIREKSATYQASTALPPPIAAAGTASLALIRENPGMRRELLD
                     KAWMIREAVAAMNFRTTRFGTPIIPLLFNSPEKANGLSQFLEENGVIVPSVNYPARQQ
                     LYLVRIAVSITHTEDQIGQLLGLLKQWKDKNESN"
     gene            4589..5662
                     /locus_tag="PLA17_04335"
     CDS             4589..5662
                     /locus_tag="PLA17_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPK84675.1"
                     /translation="MKAIKIRKVVIEPLNLDLEEPFRISIGTKYSIENALVTVILDNG
                     IEGYGEAAPLEPINGENQSTVLATLKSCVDFIKGKEITEYRSIAATLRGVFQPQVTAR
                     CAIEMALIDAYAKVLGIPLYRFFGGVGNRVETDYTIDIVPPDVAKRNAAKLAKEGYTV
                     LKTKVGKKLTDDIDRLLAIRDGAPQCGITIDANQGYSPCEAVHFTEEMTKNGIRPILF
                     EQPVSKYDLAGMRFVKDHTSIPVAADESVFTSADAINVVRTGCADYINIKLMKSGIIE
                     AMDIAAIARSANIKLMIGCMLESKLALGCAVHMVAGMGCFSHVDLDPHSEPEKEPFAG
                     GPDYRAPFYTLSPELHGIGCTRK"
     gene            complement(5684..8353)
                     /locus_tag="PLA17_04340"
     CDS             complement(5684..8353)
                     /locus_tag="PLA17_04340"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84676.1"
                     /translation="MKHSLSLLFFLSIAFLSCNRGNTPLYKSEMFAVYPDRVVQEGFT
                     STVVSRGEMNSDYRSPGAAIDPVIEFKFSINGRDNELPFGVNHQANIFPVNGELTVLD
                     IKFGQKSVPDTLRDASQPLPKNTKVRFRIDFSQVLDSFRDKGYYEDILGNRIFRDDFK
                     GLYIAGDTYPLSWDFENIPGNPDMKMEDPDGDGIFEKELTFNVFDPSAHTSSSWKLTN
                     DISQYPQFGSSSLLLDALYNMALDEMMMLMEEDGTFRTGKEWAGVWTRDISYATLLSL
                     AWLDPERCMTSLMSKVSDNRIIQDTGTGGAWPVSSDRVVWALAAWEIYKYTGDKEWLL
                     NAFGIITNSMESDIMTITDHETGLIRGESSFLDWRKQTYPLWMEPADIYGSLNLGTNV
                     AYCQALRVTGMMARELGREDNWSERAEELGEKINNHLWLEEKGYYGQYLYGRRYRTVS
                     PRAEALGESFSVLFGIADGDRSLRVLQNTPVTPYGITCIYPQIPGIPPYHNNGIWPFV
                     QAFWTMANAQERQLEAVEAGMASILRPAALFLTNKENFVAENGDFEGTETNSDRQLWS
                     VAAMLAMHHRVLMGINFEADKLNFSPVIPESFNDTYSLSGLKYRNGTYNIKVTGWGDG
                     VMSFKIDGKESADHYIPATTTGNHNIEIVMNGRTTGSTHKAVSNLTAPETPLLSVTDS
                     GLQWSQVSETTGYRVFKNGKVIASTAGTDFVPGNPDEAAEYQVMAVGSNLTESFMSNP
                     VTVSPAANGTITAEAESFNVSGENRYPGYTGSGYVKFSLSENRELRIRITAREGKYAL
                     VMRYSNGTGPVNTDNNCAIRSLYVNGIYTRALVFPQRGKDEWSDWGYTYPEWIDLKEG
                     ENILAIRYENFNRNMDGEINDFLLDRITLERFN"
     gene            8707..8841
                     /locus_tag="PLA17_04345"
     CDS             8707..8841
                     /locus_tag="PLA17_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84677.1"
                     /translation="MKILFEITSTETGKVLIKRRRIARALRWWLRDNGIAYDYIYYLI
                     "
     gene            8873..9511
                     /locus_tag="PLA17_04350"
     CDS             8873..9511
                     /locus_tag="PLA17_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84678.1"
                     /translation="MENQTLRNNAGATSDRLFGMISGRSMTAAFASLNHIFSELTAEV
                     RQDLISYLDGMGLIDDASMLVIPSTRHFFYDSEDMKGVKTIVNLKPLNYMRELRGFLC
                     RLSEMLPDDSAFVGCFTDNKAQNGFSDKYSNLPKKLSDKAEAYENGIESRIPFINRMY
                     SFIDMKTNRYLTVRTVYALLEECNLQLITMTEKNGLTYFHAKRRSAAMGLIA"
     gene            9583..10539
                     /locus_tag="PLA17_04355"
     CDS             9583..10539
                     /locus_tag="PLA17_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84679.1"
                     /translation="MKKMFYVLAALVMVAALGSCQQDDDEIMLKKKDSVSEAVLPPSD
                     AGITPVIVDGANNGGNVTCAEVAVAFNLPAGYFLCGDKIDFNDGIFDGEFPDGLNVMV
                     QDGVYVEFSMDAPLLIDGVEYVVGAAIVKGGNAANVYFYPGGSMGDKNLSSPVNRSGK
                     PAGLSNLTFCLVKANTLVIATKTYLAVPVEGEEVSYERIGWAVSGGLGVSEEYGLHMG
                     YNYYDFNGENEFELVKATLGAIEGPVGTIKVSDYWVENEHFLEVVMEIDEGFNYVFDD
                     TYLYVGSLAGYEGRWHRVFPWSIEGVFSASRVFRINFDEITL"
     gene            11320..12321
                     /locus_tag="PLA17_04360"
     CDS             11320..12321
                     /locus_tag="PLA17_04360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPK84680.1"
                     /translation="MKEPLFEYLNSNIKHQIIRGDSLSILKQIPDKKFDLVITSPPYN
                     IGKSYEVKTSIENYLSTQEEIIIELVRTLSDKGNLCWQVGNFVDKGEIFPLDIYYYQI
                     FKKFGLKLRNRIIWHFGHGLHASNRFSGRYETLLWFSKTDDYIFNLDNVRIPSKYPGK
                     LAFKGPKKGKPSGNPLGKNPSDIWEIMAQDWETGMWDIPNVKSNHPEKTIHPCQFPVE
                     LVDRCVLALTNDNSWVLDPFAGVGSAIISSIRNNRNAIGIEKEEEYCKIALKRIEDLR
                     EGKLKIRPMNKPIHKPSGNEKVSQIPKEWQQIQNQPGLSFEQKAKNENITEVFTSER"
     gene            12284..12898
                     /locus_tag="PLA17_04365"
     CDS             12284..12898
                     /locus_tag="PLA17_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HPK84681.1"
                     /translation="MKISQKYSHLNGEEYLIVHHNNLYKEIMQVVNSINATEYLTKVS
                     QEKTMPGAMLFSPIELNKAFNNEFKALGWSESRYKYYITTDQRLLPELITLPYDKQRD
                     FLISKGVTHPISSYKQTDFVKDQIAVEVQFGKYAFVAFDLFVKHLLFYSGGVINLGIE
                     ILPTKTMQSKMSSGVAYYEGEVYNILRHGRNNPPVPLLILGIEP"
     gene            13317..13838
                     /locus_tag="PLA17_04370"
     CDS             13317..13838
                     /locus_tag="PLA17_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84682.1"
                     /translation="MKLKKKRYIVLLLSMFVGYGCSNTINKNPIQTVNTKSIIDTIRL
                     KSLPSIGDILGDSIRLTGNNRGLLVNSSEICTSTTILNNGIIFDIAWDKDYRVNFIGT
                     TDSGFITNENVRVDMTLKSIKDIQNVDILKMAGWGYYIKLKSGWFAGFCIGKTCTDRG
                     LKDDDKVAFIFKR"
     gene            13950..14588
                     /locus_tag="PLA17_04375"
     CDS             13950..14588
                     /locus_tag="PLA17_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84683.1"
                     /translation="MKNYILLFVGLMLFVSCENEDIQSNPKLCSDEYFYYSGGSKTFL
                     KHSLNEVWIVFKQSDLTGELAKSILEKYSFISTDNISSDSFSGKTLAIINENCNCSDF
                     KNYLEELNKDNEISSATPVFYLSDVDPMSYWILLSEVLTKNDNERITESEFVEYAETL
                     NLELIESNYSTQHFKVKDVETGFEALEIANEIYESGKAQYSHPNFIAHMTLF"
BASE COUNT         4119 a         3215 c         3595 g         3987 t
ORIGIN      
        1 taatgagatt tacctgctgc aattgcctca tggcagcgag ggctggaaag cagcgggggg
       61 cagtagcggg ctgccccggc ccgttgccta cggtgcctca gcatcggtca gggccggcgt
      121 ggtttgcatg ggaggggaga atgaatcagg cataacagat gaagccttta tgatttcaat
      181 aacagacggc aaaccgctga ttatacctct gcctgaactc ccggttcctc tgtccggcgc
      241 agctgctgcc tcatcaggtt ccgttgtttt tatagcagga gggcagaccc ctgacggctc
      301 ttcctcatca atgtggagcc tcgatactga tgagataagc cagggatgga gaaaacatgc
      361 tgaaatgcca ctaccgttga taaacagcgt catggctgcc gttgaaggca aagaacttaa
      421 aatatggatt attggtggca gaacaagggg agagggtgat gccacatccg tgatacgtca
      481 ggagattttc agctattctc cggctgccga caggtggacc agggcaggta accttaccgg
      541 gaaaaccggt gtcatgcctc ttgcggccgg caccggggca gcaatagacg aaaggtatgt
      601 tgccctcttt ggtggcaatg acggatctgt attcaacaaa gtagaggccg ttctctctgc
      661 aatggcaaca gaatctgaca ctcttgagtt gaaacagctg aaaaatgaat atatctctct
      721 ccaggagagt catcccggtt tttccagggc agtgctgatt cttgacacaa aaaccggtag
      781 atgtattcag gcaggggaga ttcccggccc ggcacaggta accaccacag ccgtgcagac
      841 tccgtggggc attgtgatac cttcaggtga aataaaaccc ggggtgagaa ccacggcaat
      901 aagaatggcc ggattcaggt aaggcagact atggataatc acttacgagc gaggatctgc
      961 aaaaggtcat gatgaaatta cagttctggc aacactatgg atactcaatt acagtcaata
     1021 gctgagcggt acaggaatgc cttgttaaat gatataattc cattctggat gaagtactcg
     1081 cctgatcatg agcatggagg ctatttcaca tgccttgacc gtagcgggaa agtatatgac
     1141 actgacaagt tcatctggct tcagggcaga gaaatatgga tgttcagcca cctgttcaac
     1201 aatgttgaga aacgccagga atggctcgat atggcactgt caggggctgc cttccttggg
     1261 aaatacggaa gtgaccgtga tggcaacttt tatttttccc tgaccaggga agggaaacca
     1321 ctcgtccagc catacaatat tttctctgac tgttttgctg caatggcatt cggtgaactg
     1381 ttcagggcaa cgggcaagag caggtacggt gagattgcat tgaatacctt ccggaacatt
     1441 ctgaaccgtt ccgggaatcc taaaggcata tggtcaaaag tatatcccgg cacacggccg
     1501 ctgaagggtt tcacccttcc aatgatcctc agcaatctct cggaaatcat ggctcccctt
     1561 cttgatgccg ggactgttga aaggttgagg ggggagtctg ttcatgaagt catggatgtc
     1621 ttctacagca gggagcatgg cctgatcctt gagaacgtgg ctcctgacgg atcattctcc
     1681 gactcatttg aaggccggct gctgaatccc ggccacgcca tcgaggccat gtggtttatg
     1741 atggacctct ctgaaatcat gggagatcac aggcttgcag tcaaagccac agagatcacc
     1801 ctgcagacac tgagtaaggg atgggatgat aagcacggtg gcatattcta ttttctggac
     1861 gtcaaaggat atcctccgca gcaactggag tgggatcaga aactatggtg ggtgcatatc
     1921 gaggctcttg ttgcactggc aaaggggtac agactgacgg gtgacaggcg ttgtcttgac
     1981 tggtttgaaa aggttgatgc ctacacgtgg agtcactttc ccgaccctga attcggagaa
     2041 tggtacgggt acctgaaccg gagtggtgaa gtgctcctgc cgctcaaggg cggaaagtgg
     2101 aagggatgtt tccatatccc gagaggatta ttgcaggtat ggaagacatt cagtgatgtt
     2161 gaaccggtaa aatcagaata ataatgaaag agagcgtagc tgaaagaagg tatgcatggg
     2221 tggtggtggc gctgctgtgg gtggtggctt tgcttaacta tatggaccgc cagatgcttt
     2281 ctaccatgaa gaatgcaatg atggttgaca tcgtggagtt ggaaaaggcg gagaatttcg
     2341 gcaggctgat ggctgtcttc ctctggatat acgggcttat gagtcctgta gcgggaatca
     2401 ttggcgacag gctcaacagg aaatggctga tcatctgcag cctcttcgtc tggtcaggtg
     2461 tcaccttcgc catgggctat acccatgact ttggggttct ctatgccctg cgggcgataa
     2521 tgggtgtcag tgaggcgctt tacatacctg ccgggctttc cctgattgcc gattatcacc
     2581 agggatcaac acggtcactg gcggttggaa ttcacatgac cggactctat atgggacagg
     2641 cactgggtgg tttcggtgct accattgctg acagcctgtc atggcagaaa acatttcact
     2701 acttcggact cgtaggcata gtatacagtc tggtcctgat attgttcctt cgcgaataca
     2761 aacagaggca gataataaaa aaggcctcat cgggtctctc cggactcagg tcagtggctt
     2821 cggggctttc catgctgttc ggtatggtaa gcttctgggt gatactcttt tattttgccg
     2881 taccgagcct gccaggctgg gcggtcaaga actggatacc gactctcttt tcatcaagtc
     2941 tcggaattga gatgccggtg gccggaccaa tgtcaacaat aaccattgca ttctcctctt
     3001 tggttggtgt gatcttcggg gggatactct ccgacaggtg gatacagaag aacattcgcg
     3061 gacgtatcta tacgggagca ataggcctgt cgctcaccat tccctcactt ctgctggtcg
     3121 ggctcgggca cacttacctg tggatcatag gtggcgcagt gcttttcgga atagggtatg
     3181 gtatgtttga tgccaataac atgccgatcc tctgccagtt tgtgacacca cgcctgaggg
     3241 caacgggtta tggcctgatg aatatggcag gcgtatttgc cggagctttc attaccaggg
     3301 ttctcggaaa atcaatggat tccggtaacc tggcacgtga tatggctctt ctggcaatac
     3361 cggttctgct tgccgtcata cttcagctgg caatactgag acccgaaagc gcaaataaag
     3421 agtagtgaca gggtgtctga ctactatata aagattaatt ttgatacaat caataatttc
     3481 agataagcag ggagtatgat tctattgaac ggcgaagtcg ggaattacat ttacagcaac
     3541 ggcaggaagt attcttactt cgcgggaaat aactacctgg ggctggcagg tcatcctgag
     3601 ataaagaaag cagttatccg tgcggtggag aagtatggtg ttaattttgc tgcatcacgg
     3661 cacactacag gtacatcaga ccttcacctt gagctggagg gtgccctggc cgccttcaag
     3721 ggcaaggaag atgcagttgt ctttgcctca ggttaccagg gcaacagcat cctccttgag
     3781 atactgaagg ggagctattc aacgatcttt atcgaccagg cggcccatgc cagcattatt
     3841 gctgccatcc cggccgatgt ggtcagggtg atctattatg accattgtga cgccggtcat
     3901 ctcgattacc tgctcgatta taaccgggga tcgggtccgc tggtaatcac cgatggtatc
     3961 tttgccctga cgggcgaaat agctcctctg gaccagatat atgaggttgt cagaaaacac
     4021 aatgccatcc tggttgttga tgacgcgcat gctacggggg tcatcggtgc cacaggcaag
     4081 ggaacaccgg aacactttgg ccttcccggc gacggaagaa tataccagac cgaaactatg
     4141 agcaaggcgc ttggcggtta cggcggattc atatcgggca gtcgccaggt caccgggatg
     4201 atccgggaga agtcggcaac ataccaggcc tcaactgccc tgcctccgcc aatagctgct
     4261 gcgggaacag cctcgcttgc actgatcagg gaaaaccccg gaatgcgcag ggaactgctt
     4321 gacaaggcgt ggatgatcag ggaggcagtt gctgccatga atttccggac cacccgcttc
     4381 gggactccaa ttatacctct tctcttcaac agtcccgaaa aggcaaacgg actttctcaa
     4441 ttccttgaag agaacggggt tatagtgccc tctgtcaatt atccggccag acaacagctg
     4501 tatctggtga ggattgctgt atccatcact catacggagg atcagattgg tcagttactt
     4561 ggattattaa aacaatggaa ggataaaaat gaaagcaatt aagattagga aggtagttat
     4621 tgagccgttg aatcttgatc tcgaagaacc attcaggata tccatcggca caaagtacag
     4681 cattgagaat gcgctggtga cggtgatcct cgacaacgga atcgaaggtt acggtgaggc
     4741 agctccgctt gagccgatta acggtgagaa ccagtcaact gtactggcca ccctgaagag
     4801 ctgtgtggac ttcatcaaag ggaaggagat aaccgaatac aggtcgatag ctgccactct
     4861 tcgcggcgta ttccagcctc aggtgactgc acgctgtgct attgagatgg cgcttattga
     4921 tgcctatgcc aaagtgcttg ggataccgct ctaccggttc ttcggcggag tcggaaacag
     4981 ggttgagact gattacacca ttgatatagt gccgcccgat gtggcaaaaa ggaatgccgc
     5041 caaactggcg aaggagggtt ataccgtact gaagacaaag gtgggaaaga agctgacgga
     5101 tgatattgac cgcctgcttg caatcaggga tggtgctccg caatgcggaa tcacaattga
     5161 cgccaatcag ggctattcac cctgtgaagc ggttcacttc accgaggaga tgacaaagaa
     5221 tggcataaga cccatcctgt ttgagcagcc ggtctcaaaa tatgaccttg ccgggatgag
     5281 gttcgtaaag gatcacactt ccatacctgt tgccgcagat gagtcggtat tcacaagcgc
     5341 tgacgccata aacgtcgtac gcacgggttg tgcagactat atcaatatca agctgatgaa
     5401 gtcaggcatc attgaggcga tggatattgc agccattgcc aggagcgcca acatcaagct
     5461 gatgattggc tgcatgcttg aatcaaaact ggctctgggt tgtgcagtgc acatggtggc
     5521 ggggatgggc tgcttcagcc atgttgatct cgacccgcac agtgagcctg aaaaggaacc
     5581 gtttgcaggc ggacctgact acagggcgcc gttctatact ctttcaccgg agcttcacgg
     5641 gatcgggtgc accaggaaat agttgccggt aaactgctgc ccgtcagttg aacctctcca
     5701 gggttatcct gtcgaggaga aagtcattta tctccccgtc catgttcctg ttgaagtttt
     5761 cgtacctgat ggcaagaatg ttttccccct ccttcagatc gatccactca gggtatgtgt
     5821 atccccagtc tgaccattca tcctttccgc gttgagggaa tacaagcgcc ctggtataga
     5881 tgccattgac atacaatgac cgtattgcac agttgttgtc ggtgttgaca ggcccggtcc
     5941 cgttggagta gcgcatcacc agtgcatatt tcccttccct tgcggtaatt ctgattctga
     6001 gttcacggtt ctcagacagg ctgaatttca catatccgct gccggtgtat cccgggtacc
     6061 tgttttcacc actgacattg aagctctctg cttcagctgt tatggtgccg ttggctgccg
     6121 gtgagacggt aacaggattg ctcatgaagg actctgttaa gttgcttcca acagccatca
     6181 cctggtattc agcagcctca tccggattcc cgggaacgaa atctgttcct gcagttgaag
     6241 caatcacttt cccgtttttg aaaaccctgt agccggttgt ttcgcttacc tggctccact
     6301 gaagaccaga gtctgtcact gaaaggagtg gtgtctcagg tgcggtcagg ttactgacag
     6361 ccttatgggt gctgcctgtt gtccttccgt tcatgactat ctcaatgtta tggttacctg
     6421 tcgttgtggc cgggatataa tgatcagccg attcctttcc gtcaatcttg aatgacatta
     6481 ctccgtcgcc ccaccccgtg accttgatat tataggttcc gttacggtat ttcaggccgg
     6541 acagcgagta ggtgtcgttg aaactttccg gtattaccgg actgaaattc aacttgtctg
     6601 cttcgaaatt aatgcccatt aatacacggt ggtgcattgc cagcattgcc gcaacgctcc
     6661 acaactgcct gtctgagttt gtttcagttc cttcgaaatc tccgttttcg gccacgaagt
     6721 tttccttatt ggtaaggaag agtgcagcgg ggcgtaatat cgaagccatg ccggcctcaa
     6781 cggcttcaag ttgtcgctcc tgcgcattgg ccatggtcca gaaagcctgc acgaagggcc
     6841 atatcccgtt gttgtgatat ggaggtatcc cgggtatctg cggatagatg catgttatcc
     6901 cgtaaggggt gacgggggta ttctgaagca ccctgaggct cctgtctcca tctgctatac
     6961 cgaagagtac cgaaaatgat tcgccgagag cttccgcccg tggcgaaacc gtcctgtatc
     7021 gccttccgta aagatactgg ccgtaatagc ccttctcttc cagccagagg tggttgttga
     7081 tcttttcccc gagttcttcg gccctttcgc tccaattgtc ttctctgccc agttcccggg
     7141 ccatcatccc tgtcactctg agtgcctggc aataggcaac gttggtaccc aggttaagag
     7201 agccgtaaat gtctgcaggc tccatccaca gcggataggt ctgtttcctc cagtcgagga
     7261 aggatgattc accccgtatc agtcctgttt catgatcagt gattgtcatg atgtcagact
     7321 ccatgctgtt ggtgataatt ccaaaggcat tcagcagcca ctctttgtca ccggtatatt
     7381 tatatatttc ccatgcagcc agtgcccata cgaccctgtc ggaagaaacc ggccatgctc
     7441 cgccggtgcc ggtatcctgt attatcctgt tatcagacac tttgctcatg aggctggtca
     7501 tgcatctttc cgggtcaagc caggccagtg agagaagggt ggcatagctt atatcgcggg
     7561 tccagacccc cgcccattct ttgccggtac gaaatgttcc atcctcctcc attagcatca
     7621 tcatctcgtc gagtgccatg ttataaagtg catcgagcag gagggaagag cttccgaact
     7681 gagggtattg tgaaatgtca tttgtaagtt tccatgatga tgaagtatgc gcagaagggt
     7741 cgaagacatt gaatgtcagt tctttctcaa agatgccgtc gccgtcggga tcctccatct
     7801 tcatatccgg gttccccggt atattctcga agtcccagct gagcgggtag gtatcaccgg
     7861 caatgtaaag tcccttgaag tcgtccctga atatccgatt cccaagaata tcctcataat
     7921 atcccttatc cctgaatgag tccagcacct ggctgaagtc gatcctgaac ctcacttttg
     7981 tgttttttgg caggggctgt gaggcatctc tgagcgtgtc cgggaccgat ttttgcccga
     8041 attttatatc cagaaccgtt aactccccat tcacgggaaa gatgttggcc tgatggttta
     8101 ccccaaatgg cagttcgtta tcacggccgt tgatgctgaa cttgaactca atcacagggt
     8161 caatggctgc cccggggctc cggtaatcag agttcatctc acctctgctt acaactgtgg
     8221 aggtaaaccc ttcctgtacc acacggtcgg ggtagaccgc aaacatttcc gatttgtaca
     8281 ggggagtatt tccacgattg catgacaaaa acgcgattga caggaagaaa agaaggctca
     8341 aagaatgttt catgaacatg atttctctat tatttcgcaa atttaatcca aattaatcat
     8401 ccgttcgtca aaatttagat tcatgctgtt ttttggtttc agattcatat aagaataaga
     8461 tactgagcgc attgaaatac aaattattga ttttcccccg gaaattcgga ttacgctggt
     8521 aaaatagagc ctctttttga cgaatgcaag gttaaatggt gtgagcggac tctctgctcc
     8581 gacggatgcc ccataatgct tgacaaaggt ttttaaaaac agtcgtaaat tgccattaca
     8641 tgggcgtttg cgacttgaca cgccttggta tgtgctttaa atttgtcata actaaaagag
     8701 cgaaaaatga agatactttt tgaaatcaca tcaaccgaga cgggcaaggt gctcataaag
     8761 cgcaggagga tagccagggc attacgctgg tggctcagag acaatggtat tgcatatgac
     8821 tatatctatt atctgattta gaaatttaga aaacacaata aaatataaag agatggaaaa
     8881 tcaaacctta agaaacaatg caggtgcaac ttctgaccgg ttatttggta tgatctccgg
     8941 acgcagcatg acggctgcgt ttgcaagttt aaaccatata ttttccgagc tcactgcaga
     9001 agtgcgccag gatctgatca gctatcttga cgggatgggg ctgattgacg atgcttccat
     9061 gctggtcatc ccatcgacaa ggcatttttt ctatgatagc gaggacatga agggagtgaa
     9121 gaccatcgtg aatctgaaac cgcttaatta catgagggag ttaagaggtt tcctgtgcag
     9181 gctatcagaa atgcttcctg acgacagcgc atttgtcgga tgctttactg acaacaaggc
     9241 gcagaacggt ttctccgaca aatacagcaa tctgcccaaa aaactttcgg acaaggctga
     9301 agcttatgag aatggcattg aatcaaggat accttttatt aacaggatgt acagctttat
     9361 tgatatgaag accaacaggt atctgacagt aaggacagtc tacgctctcc ttgaggaatg
     9421 caatctgcag ctgattacca tgaccgagaa aaacggcctg acctattttc atgcaaagcg
     9481 caggtctgca gctatggggc tgatagcgtg acagatatat tcaaaaatat taatagacaa
     9541 atatttatta tttaattaat tactcactaa aaaatggctg agatgaaaaa aatgttttat
     9601 gtgctcgcgg ctcttgtaat ggtagctgcg ctgggttcct gccagcagga tgacgacgaa
     9661 attatgctga aaaagaaaga ttctgtctct gaggcagtac ttccgccttc ggatgctggt
     9721 ataacgcctg tgattgtaga cggtgccaat aatggaggca atgttacatg cgctgaggtg
     9781 gctgtagcat ttaacctgcc cgcaggatat ttcctatgcg gggacaagat tgactttaat
     9841 gacggaatat ttgacggtga gtttccggat ggcctgaacg tgatggtaca ggatggcgtt
     9901 tatgtggaat tcagcatgga tgcacctctt cttattgatg gcgtggaata tgttgttggt
     9961 gcagcaattg taaaaggagg taacgctgcc aatgtctatt tctatcccgg cggcagtatg
    10021 ggcgacaaga atctcagctc accagttaac aggtccggca agcccgccgg cctgagcaac
    10081 ctgaccttct gcctggtcaa agccaatacg cttgtgattg ccactaaaac ctacctggca
    10141 gtaccggtag agggagaaga ggtctcctat gaaagaatcg gatgggctgt tagtggcgga
    10201 ttgggtgtca gtgaagaata cggacttcat atgggataca attactatga cttcaacggc
    10261 gaaaatgagt ttgagcttgt caaggctaca ctcggagcga ttgaaggtcc tgtgggaact
    10321 atcaaggtat ccgattactg ggtggaaaat gaacatttcc ttgaggtagt tatggaaatt
    10381 gacgagggtt tcaactatgt gttcgacgat acctatcttt atgtaggatc gcttgccggc
    10441 tacgagggaa gatggcacag ggtattccca tggtctattg aaggagtctt ttctgcctcc
    10501 agagttttca gaatcaactt tgatgagatt acattgtaga atggagagat aagtttctgt
    10561 aaaaatgggt cggccataat agccggccct ttttttatca ccctgtcatg ccttttgact
    10621 gattataacg gaccacttcc attaataaga gcagccctca cttttctgtc ccctctccgg
    10681 gaaactttct gaagttgtga gggctgcctt tcaaaaataa aaaggacctt cggaagtata
    10741 aattaacaaa aaatatatga caaaaccatg tggcagtatg ctgattttga ccaatatagg
    10801 ctattcaatg atcaacaggt gccaggcatt tacaaacgaa gctttcatga tctcactact
    10861 gaggtcaaaa gcccttttca gtgcgtatct ataagaattt cagcttgcat cagattgtta
    10921 aaatattctt gtgtatcacg atttcgttat gattcccgga aggttgcaaa aggagttttg
    10981 tttaacttgt atcgaagttt gaacgtggaa agtcagtcag cagtaatttt cactgcaagt
    11041 agtttgtgga tagcataatc tgcagtgtga ctgacggggc attgccgtat caggagtcac
    11101 gggaagttgg ctttcgtaca tcaactatca gaattggaat atacacgttg aagctgacat
    11161 taagtcacta acagtaaatt atctacacct tttgtataca tattataaag ttaactgcaa
    11221 cgctcagata cgaatcatag tgaataactt tcttttgaaa aagtagtcat tcagctattc
    11281 aatctgtatt tttgaaatca atacactaaa aacattctcg tgaaagagcc attatttgaa
    11341 tatttgaatt ccaatatcaa acaccagata atcagaggag atagtctgtc aattctcaaa
    11401 cagatacctg acaagaaatt tgatcttgtt attacttcgc ctccgtataa tattggcaaa
    11461 tcctatgaag ttaaaactag cattgagaac tatctctcca ctcaggaaga aataataatt
    11521 gaacttgtaa ggacactttc agataaaggt aatctatgct ggcaggttgg taattttgtt
    11581 gataaaggag aaatattccc tctcgacatt tattactatc agatattcaa gaaatttgga
    11641 cttaaactca ggaatagaat tatctggcac tttggacatg gacttcatgc tagtaacagg
    11701 ttctccggaa gatatgagac actattgtgg ttcagtaaaa ctgatgatta tatctttaat
    11761 ctggataatg tcagaattcc ctcaaaatac cctggcaaac tggcttttaa gggaccaaag
    11821 aaaggtaagc catctggaaa tcctctgggg aaaaatccta gtgatatttg ggaaatcatg
    11881 gcacaggatt gggaaacagg tatgtgggat attccaaatg taaaatcaaa tcatccagaa
    11941 aaaacaatac atccttgtca attcccggta gagcttgttg atagatgtgt attggcatta
    12001 actaatgata atagttgggt ccttgatcca tttgctggag ttggttcggc aataatctct
    12061 tccattagaa acaataggaa tgcaataggt attgaaaaag aagaagaata ctgcaaaatt
    12121 gccctcaaga gaatagaaga cttaagagaa ggcaagttga agataaggcc aatgaataag
    12181 cctattcata aaccatcagg taatgaaaaa gtgtcacaaa ttcccaaaga gtggcaacaa
    12241 atacagaacc aacctggtct ttcattcgaa caaaaagcca aaaatgaaaa tatcacagaa
    12301 gtattcacat ctgaacggtg aagaatactt aattgtccat cataataacc tgtataaaga
    12361 aataatgcag gtggttaata gtataaatgc cacagagtac ctgacaaagg taagccagga
    12421 aaaaactatg cctggggcaa tgcttttcag cccaattgag cttaataaag cctttaacaa
    12481 tgaatttaaa gctttaggct ggtctgaaag tagatacaag tattatatta caacagatca
    12541 gagacttctt ccagaattga taacccttcc ttatgataaa cagagggact ttcttatctc
    12601 taaaggagtt acccatccta tttcttcgta taaacaaaca gattttgtaa aagatcaaat
    12661 agcggttgaa gttcaatttg gaaaatatgc ttttgttgcc tttgatctgt tcgtaaaaca
    12721 cttgcttttc tactcaggtg gagtaataaa tcttggtatt gaaattttgc cgaccaaaac
    12781 catgcaatca aagatgagta gcggagtagc atattatgaa ggtgaggttt acaatatcct
    12841 cagacatggt cgtaacaatc cacctgtacc attgctcatt cttggcatag aaccttgaag
    12901 ggaactgtag ttaacaggtc ggtatatgtc attggctttt ccgcaccgcg actggttttt
    12961 gcgcgccgat ccaacagacc ggcacaccaa aaccagttgg tgctttatcg gcccggccta
    13021 gcggctgaag ccgccgggca aaaaccattg cgcggtgcgg ccctgccccg gctcccgcca
    13081 gggtctccct catggcgtca cgatttttta cgtaccccaa aactttgtta tgaaaagaat
    13141 aaaactacat accatcacga gtgcaaaaaa agcactatag attgtttatt ccggccatat
    13201 ctcgtgaccc tggccaaaag cagattccct gtcacggctt ccattatcaa aacaacaaaa
    13261 acgccttata atgccgcgcc atcagcacca aattaaaatg aaccgctgct attaacatga
    13321 aacttaaaaa aaagagatat atagtacttt tattaagtat gtttgttgga tatggatgtt
    13381 caaatactat caataaaaat ccaattcaga cagtaaatac aaaatctatt attgatacaa
    13441 tccgactgaa aagtttacct tcaattggag acattcttgg tgattcaatt agattgaccg
    13501 gtaataatag aggactcctg gttaatagct ctgaaatttg cacttcaaca accatcttaa
    13561 ataatggaat aatttttgat attgcctggg ataaggatta ccgggtaaac tttattggga
    13621 ctactgattc cggttttata actaatgaga atgtcagggt agatatgacg ttgaaaagta
    13681 ttaaagatat tcagaatgtt gatattttaa agatggcagg atggggttat tatattaaac
    13741 taaaatcagg ctggtttgca ggattctgta ttggtaaaac atgcacagat agaggattaa
    13801 aagatgatga taaagtagca ttcattttta aaagataatt aactggtgct aaccaggcgg
    13861 tatctgcaat tgccttcggc tccgtagaac gtcagatgca agtttgacgc aacgtaaaga
    13921 gtaagaaaat catctggtaa taaagagtta tgaaaaatta tattctatta tttgtcggac
    13981 tgatgctctt tgtttcttgt gaaaacgaag atattcagtc aaatccaaaa ttatgttcgg
    14041 atgaatattt ttattattcc ggaggttcaa aaacatttct aaaacattca ttaaatgaag
    14101 tatggattgt atttaagcaa agcgacttaa caggagaatt ggcgaagtca attttagaaa
    14161 agtattcctt cataagcaca gacaatatct cctctgacag tttctctggc aaaaccctgg
    14221 caattattaa tgaaaattgc aattgttcag acttcaaaaa ttatctggag gaattaaata
    14281 aggataatga aatatcttct gccacaccag tattttattt gtccgacgtt gaccctatga
    14341 gttattggat tttactaagt gaagtgctta ctaagaatga caatgaaaga ataactgaat
    14401 cggaatttgt agagtatgca gaaacgttaa atttagaatt gattgaatca aattattcaa
    14461 cacaacactt taaagttaag gatgtagaaa cgggatttga agcgttggag atagctaatg
    14521 agatttacga aagtgggaaa gctcaatatt cgcatccaaa ttttattgca cacatgacat
    14581 tattttaaaa ccggcacctg acaaagttca tactgcaggg cgaagtccgg tgatacgcca
    14641 actgcggatt cccgttccac agcctcggct ctgccgaacg ttaccagcaa gccagagtag
    14701 tcttgagaaa aaaaataagc acaagtgcac aaccttttag acacaattcg catcttttaa
    14761 taagtgcgag attgtcattc aatctgcctt aactaattat taatgaaata taaaaatata
    14821 ttcattctat atttcagcat agtactgcta accgcattca gttgtgaacg caatgatgat
    14881 atcattgata atggcttgtc tgagcccagg cctgct
//
LOCUS       DAOPBK010000049        14868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4070_length_14868_cov_5.027206, whole genome shotgun sequence.
ACCESSION   DAOPBK010000049 DAOPBK010000000
VERSION     DAOPBK010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14868
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_14868_cov_5.027206"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="PLA17_04380"
     CDS             <1..1722
                     /locus_tag="PLA17_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK84684.1"
                     /translation="YNACQTGTDEATYAQSADGNFKDHDLSGVGSLTPTSSRSDVLWG
                     TAFSNINTASGVIENAAAVGVSDALIAEARFFRAFDYFLLVQTFGGVPLDLGSGELMF
                     NTTPVRVSVRNTVPEVYTKAIFPDFIKAVNDLPETPRVPGAVTKTVARLHLAKAYLTY
                     GWWLENPNSIPTYPAADRVDPDGHNAQWYFQQAYDIAVTAIKNPGPYGLMETFYDVNL
                     AQNDRNKEILLWADHTQESEFYNGGSLTYGSGGAPDNFAGWMMTWNYTVIRSSGVSSV
                     QREAEQHLGRPWTRMCPTIGVIENTFADKVNDSRYDGTFTTVYRGNWPKGGVTAPSLV
                     NANGMSVVPGDAILTFLNDNDPGVVYDVANANIGAGVLPGRADFVIAPRGISRVVYPG
                     LWKLGPYRTDNGNGLGQPNAGSTRPYNIAKFSELYLVAAEAAVKGATTGAGMTAYDLV
                     NVLRARAGIWRWDNNGNIAKTADNSAEMVAATPATITVDYILGERSREFYGEGYRWFD
                     LVRTQTWASKAATYEICGASYGDHTPVTFTRTIEPKHYLRPIPQGQIDGMEMTAEEKA
                     AYQNPGY"
     gene            1924..2589
                     /locus_tag="PLA17_04385"
     CDS             1924..2589
                     /locus_tag="PLA17_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HPK84685.1"
                     /translation="MTEILERISTCVELGKVDIKSPYPPAMKGQEGAFELTRLALDEG
                     VSPGDILEKALIPAMSRVGQKFSRNEIFVPQMLMAAKAMSSSMTHIKPFYLSGETKRR
                     GTFIIGTVKGDLHDIGKNLVSMMIEGSGWEIVDLGVDVSTEKFIEAVEKHPGSVVGLS
                     ALLTTTMENMRKSVEVLKNRFPAMKILVGGAPVTEEFCRKIGADFYSPDPQGAVEYLR
                     TLT"
     gene            2710..3555
                     /locus_tag="PLA17_04390"
     CDS             2710..3555
                     /locus_tag="PLA17_04390"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPK84686.1"
                     /translation="MKTILKGTSKEVVIDTEGPVVIIGECINPTRRKKLASTLQANDF
                     SYMLELAKSQIDAMADVLDVNVGFPGVVDEELLPRAVIELQERFDIPLCLDSPNPKAI
                     EAALKVARGKCLINSVNAEEASLQRLLPVAREYGAAIIGLVLDDDGITHDPEKRLRIA
                     EKIIERAVRSGIKEEDVIIDPMAMAVSADPNACRATLETIRLVHDRLGHNITQGASNI
                     SFGLPNRENINNAYMAMSIMYGLTCPIANPEKITGLVRAADLVLGRDDYAIRFIEHFQ
                     LTQLP"
     gene            3555..4241
                     /locus_tag="PLA17_04395"
     CDS             3555..4241
                     /locus_tag="PLA17_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPK84687.1"
                     /translation="MRLSYRYSELLPSVDALAGVMGYLPGTAPEPVTDLIAGLSEELM
                     TTGEARAEYTIFDHVKLDPLSKTVEVEGVVFHVKPIVFSQIKNAESVALFICTAGSEV
                     DELSRKSMKEGDLLRGYVYDVIGSEVAEGAADRMQESMKTAVASSGMSITNRFSPGYC
                     GWDVAEQHKLFTFFRENYCGITLTDSALMDPVKSVSGMIGIGSRVKYSPYRCHQCDDK
                     NCIYRGKKNR"
     gene            complement(4378..6321)
                     /locus_tag="PLA17_04400"
     CDS             complement(4378..6321)
                     /locus_tag="PLA17_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPK84688.1"
                     /translation="MKTKLDILSAILFASLITIPLNSQVRLPRLISDGMVLQRDTELK
                     IWGWAAPGEKVTVEFAGKQYTNTTDSSGEWMVTLLPMQAGGPHTMTIVASNKLTVSNI
                     LIGDVWLCSGQSNMELPVRRVRPLYEAEIAAAENNSIRSFTVPKRFVFTGPESDLPGG
                     EWRAANPETVLDFSSAAWFFAREINQTCGVPVGILLSAFGGSPAEAWISEESLEAFPE
                     HYAELRKLNEESYISNIEKEDRRRIADWYSNLQKEDLAYRAGGLRWSDIDPDSDDWSS
                     FTVPGFFSATPLKGINGVVWFRKEIDIPASAAGQPGTVNLGRIVDADSAFLNGTFIGT
                     VSYQYPPRWYTVPAGIVKEGKNILTVRLISNIGDAGFVPDKTYELTAGDFKTSLEGEW
                     KYKVGAVLPPLRGQTFFGYKPAGLYNAMLAPLLNYSIKGILWYQGESNTGRPEEYRSL
                     LPAMIRNWRNSLGQGDVPFLVVQLPNFMESRETPYDSGWALFREAQTEALELPATGMA
                     VTYDIGEWNDIHPLNKKDVGHRLALIARKVAYGDSEVVASGPTYKSMRVRGRRIIISF
                     DNTGSGLTVRNGKRPGHFAIAGEDMQFVWAKARIKGDRVIVRARKVKRPVAVRYAWAD
                     NPEGANLYNREGLPAAPFRTDGW"
     gene            complement(6538..9411)
                     /locus_tag="PLA17_04405"
     CDS             complement(6538..9411)
                     /locus_tag="PLA17_04405"
                     /inference="COORDINATES: protein motif:HMM:NF027309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HPK84689.1"
                     /translation="MIISAGKRAEAIILASAGSCAPLVVSPGEWPGVTRALADLQNDL
                     SLVTGAMPDLITASKPPRAEFMIIAGTIGKSAAIDRLTKRKKIDVSAIKDKWESFIIQ
                     TVEKPFPGVKSALVIAGSNKRGTIYGIYEISRQAGVSPWHWWADVPAGKSSELYVIPG
                     RHVWGEPSVKYRGIFLNDEYPALTRWVAFKYGNVTPSVNPPVPPDVANYGSEFYSRIF
                     ELLLRLKANYLWPAMWNNAFNEDDLRNASLADEYGIVMGTSHQEPMIRAQKEWDRRYL
                     KTLGNWSWTHHEDTLVKFWREGIRRNKDYESIITIGLRGADDTEMGPGGPAANIEKLE
                     KIVEVQREMIAEEIDSDVTKVPQSWCLYKEVQDYYHEGMRVPDDVTLLWAEDNWGNVR
                     RLPVANERKRSGGAGIYYHFDYHGGPRSYQWINTSPIPKIWDQMSLAKQYGADRIWIV
                     NVGHFRGYEIPLEYFLDLAWDTGAMGSKGMSAWTEQWAADVFGPEHASEISEIIRDYT
                     MFNGRRKPELLSPSTYSLVDYHEAERVVEEYNRLADKAEHIGHLLPAEMKDAYFHLVL
                     FPVKACALVNELYVTAGKNALYASQGRANAGKMADRTEQLFAADTALMGYYNRVYAGG
                     RWKHFMDQAHLGYIAWNDPPVNSLRHIRLERPVPAQGALPAITAEGSAEAWPGSATTP
                     LLPPFDVFNKQTRYFELFNRGDQPFVYTASADHPWIILSSSHGTVTDQERVDVSINWP
                     ELPYGQHNGTVTVAAERSEFKIGITAFRPEEPDPETVEGFVEADGYVSMEAVSYTGRH
                     DSGERGWEQIEDYGRTGSGMRARAFTDAPPAVPGKDSPVLEYRIYFFSAGEFETVLYM
                     APSLNFLPDRNFRIGLSVDDSEPQLITVVPAGFNAENFNREWEETVRNSIRYVKGKIM
                     VAGPGYHTLKVWMIDPGMVLEKVVVNTGGLRSSYLGPPESYSR"
     gene            complement(9717..11102)
                     /locus_tag="PLA17_04410"
     CDS             complement(9717..11102)
                     /locus_tag="PLA17_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84690.1"
                     /translation="MKRAILLVITTGMVLLYQSCSEPEVPEKPDIDPVITNIAIDPAV
                     TYQEMIGFGGSLTWYSDRITSSPHKEQICTLLFEDLGTDIIRFKNNYYPLGYPAVKSP
                     ATMENGSIKTLWSVTGDLYAIAKQLNPAIQTLVSSWTPPSALKSNGNLREGTLKKDKG
                     AFVYEAFGEYWNDLLDNLQFNPDYISIQNEPGWITPDWETCEWRPVETTEFAGYDAAF
                     DAVYNKIMTRTDPPKMIGPEAENIGYSGKLGGNTFAVFSDPLKNKPHLEAYAYHTYNY
                     TASTLIAQTKADLNMIRDSYGNKPCIMTEYSNFTWFNTAWFIIQNINEANAAGYIYWL
                     MAWDDSNNDSMIRLTPSGTFTVTPFYYMMKHFSKEIDRGYFRVKVSSQSVPMSAFIDP
                     SGRKITVVAVNPTIDAVNYKFEVTGRTITSIRAFQTDGINNYREIQPLSSDKYITLKS
                     KSVTTVVLDLL"
     gene            complement(11174..13447)
                     /locus_tag="PLA17_04415"
     CDS             complement(11174..13447)
                     /locus_tag="PLA17_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPK84691.1"
                     /translation="MKKTVNAPYKDPSLSIKKRVEDLMGRMTLEEKCAQLMGLWNGGV
                     EDFKPEIFSDPAKMKEIFGLGCHSVHPAPFGIKDTVTMRNAIQKYLVEETRLGIPALF
                     VDEGQHGMMRPEATVFPQAIGLACSWDTALFEKVYGIVAHEMRSRGTHHALTPVIDVC
                     RDPRWGRTEETYGEDPYLNGMLSIAAVKGLQGSDNGKVDKNNVAATLKHLVGHGESEG
                     GLNQAPANHSIRVLRDYHMPPFKMCIDHAKPVSIMPSYNEVDGVPSHANRWLLKDLLR
                     DEWGYKGMIVSDYNGVEHLFNKHFVCTDAVDATITAFNSGVQFEFPQANLYRLLPELV
                     RKRKVKKEDVDHAVEQALAFKFSMGLFENPYIDEKEAVAVSKSLMHRKTALKAAHESI
                     VLLKNDGLLPLKKGKYKKIAVIGPCAKDVWFGGYSGEPYEKVSLLDGIRAKAGSGTEV
                     LFAQGCRLTINTTLSYFNWKYDEIIFASREENLKLIKEAVKVAKEADLVILALGENEH
                     LCREAWAQNHIGDNMTLDLFGEQNELADAITALGKPVVLYLANGRPLSINKLAETVPA
                     IVEGWYMGQETGTAAADIIFGEVNPSGKLTITFPKSVGQIPMYYNHKPSARWQDYISL
                     DIKPLFHFGYGLSYTKFKYGKPVLKKTTIKKSEATKVMVEVKNAGKVAGDEIVQLYIR
                     DMVSSVTRPVKELKGFARISLKPGETKKVTFEITPDKLAFHNIDMKYVVEPGDFEIMT
                     GPSSRDEDLQKTMLTVV"
     gene            complement(13461..14579)
                     /locus_tag="PLA17_04420"
     CDS             complement(13461..14579)
                     /locus_tag="PLA17_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPK84692.1"
                     /translation="MKKIFMPAVAAIAIMLSACGNNDITLKKAFEDHFLIGTAMNGMQ
                     ISGTDPQALPFIASQFNSVTAENEMKWERIHPEPGVYNFGLADSIVDFAEQNGMKVIG
                     HTLVWHSQTPRWVFQDSLGNQLTRDALLERMREHIHTVVGHYKGRVLGWDVVNEALNE
                     DGTLRNTKWLEIIGEDYIEKAFIFANEADPDAELYYNDYNIEEPAKLAGVIPIIENLQ
                     AKGIRIDGVGIQGHWHLDSPDLAIIDESIGLYGALGIKVMITEMEINVLPTPTRFFGA
                     EISNMGAYLDSLNPYVEGLPDSVQMLLTNRYEEVFRILLKHDDLITRVTFWGVHDGYS
                     WKNGWPIPGRTNYPLLFNRDYTPKPAYDAVIREVKKLK"
     gene            complement(14629..>14868)
                     /locus_tag="PLA17_04425"
     CDS             complement(14629..>14868)
                     /locus_tag="PLA17_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK84693.1"
                     /translation="KVGLAVGGALAGWLLNGYGYVANVAQTATAVQGIRLSVSIFPVI
                     ALVFCVVALFRYDIGKKVEYQMQDELAERRKAYTE"
BASE COUNT         3396 a         3869 c         3734 g         3869 t
ORIGIN      
        1 tacaatgcct gtcagaccgg taccgatgaa gccacctatg cccagagcgc tgacggcaac
       61 ttcaaggatc atgacctctc gggtgttgga tctcttaccc ctacttcaag caggtcagat
      121 gtactctggg gaacggcatt ctccaatata aacaccgcca gcggagtgat tgagaatgct
      181 gcagctgttg gtgtttctga cgctctgata gccgaagcaa gattcttccg cgccttcgac
      241 tatttcctcc tggtgcagac tttcggaggt gtgccgcttg accttggctc gggtgaactc
      301 atgttcaata ccactcctgt cagggtatcc gttcgcaaca ccgtaccgga ggtttacaca
      361 aaggcaatat tccctgattt tatcaaggct gtgaatgatc tgccggaaac tccccgtgtt
      421 ccgggagccg tgacaaagac cgtggcacgc ctgcatctgg caaaggcata ccttacctac
      481 ggatggtggc ttgagaatcc aaacagtatt ccgacttatc ctgcggctga cagggttgat
      541 cctgacgggc ataatgcaca gtggtatttc cagcaggcat atgatattgc agtaaccgca
      601 ataaagaatc ctggtcctta cggcttaatg gagacattct atgatgttaa ccttgctcag
      661 aatgaccgta acaaggagat acttctctgg gctgaccata cccaggaaag tgaattctac
      721 aacggaggaa gtctgactta tgggagcggc ggggctccgg acaactttgc cggatggatg
      781 atgacctgga actacacagt cataagaagt tcaggagtaa gctcggttca gcgtgaggct
      841 gaacagcatc tgggccgtcc atggacccgc atgtgcccga cgattggtgt aattgagaat
      901 acatttgccg ataaggtcaa tgactcacgg tatgacggta cgttcacaac tgtttaccgt
      961 ggcaactggc ccaaaggcgg agtcaccgcc ccttcacttg tgaatgccaa cggcatgtcg
     1021 gtagttccgg gtgacgcaat tctgaccttc ctgaatgata acgatccggg agtagtctat
     1081 gacgttgcaa atgccaacat cggagccggt gtgctgcctg gcagagctga cttcgtaatt
     1141 gccccgcgcg gcatcagcag agtggtctat cccggccttt ggaagcttgg tccataccgc
     1201 acggataacg gcaacggact ggggcagccc aatgccggct caacccgtcc gtacaacatt
     1261 gcaaagttct ctgaactata cctggtggca gcagaggcag ctgtcaaggg tgcaacaacc
     1321 ggggccggaa tgacagcata tgacctggtt aacgtgcttc gcgcgcgcgc tggcatatgg
     1381 cgctgggata acaacggaaa catagccaaa acagcggata acagtgccga aatggtggca
     1441 gccactcctg caacaattac tgttgattat attctcggtg aaagatcacg ggagttttat
     1501 ggcgaaggct accgctggtt tgacctcgtc cgtacacaga catgggcttc caaggcggct
     1561 acctatgaaa tctgcggcgc aagctacggt gaccatactc cggtaacctt tacccgtacc
     1621 atcgagccaa aacattatct gaggcctata ccccagggtc agattgatgg aatggaaatg
     1681 acagctgagg aaaaggctgc ctatcagaat cccggatatt gatcatccgt gatgtttttg
     1741 acatacagca ttgaaaaacc aacgactgtt tctatttgaa tatatggtta gagttttaga
     1801 ttagggttgg ttcaggttaa agtgtccgtg gttttgtcca cggacacttt ttacctgttt
     1861 ttcttgtttc tttgtaaaag cctgccacca gggcagtcaa ggtttattgt aatgattcct
     1921 gagatgactg agatacttga gagaatctca acctgtgttg aattaggcaa ggttgatatc
     1981 aaatcaccct atcctcccgc aatgaagggg caggagggtg cctttgagct tacaaggctt
     2041 gcccttgatg agggagtgag cccgggagac atactcgaga aagcgctgat tccggcaatg
     2101 tcaagggttg ggcagaagtt ttcaagaaat gagatattcg tgcctcagat gcttatggca
     2161 gccaaggcta tgagcagctc aatgacccat atcaaaccct tttacctctc gggagagaca
     2221 aaaaggagag gcaccttcat aattggtacc gtcaagggcg accttcatga cataggcaag
     2281 aacctggtct caatgatgat agaaggatcg ggctgggaga ttgtcgatct gggggttgat
     2341 gtcagcacgg agaaattcat cgaagctgtt gagaagcatc cgggctccgt ggtcggactc
     2401 tctgcccttc tgacaacaac catggaaaac atgcgcaaaa gtgtggaggt cctgaaaaac
     2461 agattccctg ccatgaaaat ccttgtcggg ggggcgccgg tcacggagga gttctgcaga
     2521 aagatcggag ccgattttta ctctcctgat ccccagggag cagttgaata tctcagaacc
     2581 ctgacctgaa tcaaataatt atttgttatt tcgctttgca gtgtacggaa ccaatacggt
     2641 gacgatgcac cggaattgtt ttctggcaat agttgccggg catatacata taatcttgat
     2701 aatcagacaa tgaagacaat tctgaaggga acgtcaaaag aggtggtaat tgataccgaa
     2761 ggtccggtag tgattatcgg agaatgcatc aatccgacaa ggcggaagaa actggcatca
     2821 acgcttcagg caaatgattt cagctatatg ctggagcttg caaagtcaca gattgatgcc
     2881 atggcagatg tccttgacgt aaatgtcggc tttccgggag ttgttgatga ggaattattg
     2941 ccacgggcgg ttattgaact gcaggagcgc tttgacatac ctctgtgtct tgactcaccc
     3001 aatccgaaag ccatcgaggc tgccctgaag gtagccaggg gcaagtgcct gataaattct
     3061 gttaatgcag aggaagcctc cctgcagaga ctgcttccgg tggcccggga atacggggca
     3121 gcgataatag gactggtgct tgatgatgat ggcataaccc atgatccgga gaagcgactg
     3181 aggattgccg aaaagataat tgagagggcg gtcagatcag gaattaaaga ggaggatgtg
     3241 atcattgacc cgatggcgat ggctgtcagc gctgacccca acgcctgcag ggctaccctt
     3301 gagaccatca gactggttca tgacaggctt ggacacaaca taacacaggg agccagtaat
     3361 atctcattcg gactgcctaa ccgcgagaac atcaataacg cctacatggc catgtcaatc
     3421 atgtacggcc tcacatgccc cattgccaat cctgagaaga taacagggct ggtaagagct
     3481 gctgatctgg tactcgggag agatgactat gcgatcaggt tcatagaaca cttccagttg
     3541 acacaactgc cctgatgagg ttaagctaca gatacagtga acttctgccc tctgttgacg
     3601 ccctggccgg ggtcatggga tacctgcccg gcaccgcacc tgagccggta acagatctca
     3661 ttgccgggct gtctgaggag cttatgacta caggagaggc cagggcagag tacaccatct
     3721 tcgatcatgt caaacttgac ccgctcagta aaactgttga ggttgagggc gttgtttttc
     3781 atgttaagcc aattgtcttc agccagataa agaatgccga gagtgttgca ctcttcatat
     3841 gcaccgccgg cagtgaagtg gatgaactca gccggaaaag catgaaggag ggtgatcttc
     3901 tgcgtggtta tgtttatgat gtgataggat cagaggtggc ggagggagcc gccgacagga
     3961 tgcaggagag tatgaaaacc gctgtagcat catcaggtat gagcattacc aaccgcttca
     4021 gcccgggtta ctgcggatgg gatgtggctg aacagcataa actcttcact ttcttcaggg
     4081 agaactattg cggcatcacc ctcaccgact ctgccctaat ggatcctgtg aagtcagtca
     4141 gcgggatgat cggtatcggg agcagggtga agtattcacc ttaccggtgc catcagtgcg
     4201 atgacaagaa ctgcatctac cgggggaaga aaaaccggta gaggccggca gtttccggtc
     4261 agccgttgcc ccccggcctg ctatccggac tcggtgctgc gtgcaggtcg ctgactccct
     4321 gacctgttat caggtgtcag tgctgagtgc aggccgctgc ccccctgatc tgacctgcta
     4381 ccagccatcg gtacggaacg gtgctgcagg caacccttcc ctgttataga gatttgctcc
     4441 ttcagggtta tcagcccagg cataccttac ggctaccggt ctcttcactt ttcttgccct
     4501 tacaatcacc ctgtcgcctt ttatccttgc tttcgcccat acgaactgca tatcctcgcc
     4561 ggcgatggcg aaatgccccg gtcttttacc gttacgaacc gtcagtcccg atcctgtatt
     4621 gtcaaacgaa atgatgattc tccggcccct gactctcatt gacttgtatg tggggcctga
     4681 agctaccacc tctgaatcac cgtatgctac ttttctggca atcagggcca gcctatgtcc
     4741 cacgtccttc ttgttaagcg gatggatgtc attccattct cctatgtcat atgtgaccgc
     4801 cattcccgtt gccggcagtt caagcgcctc tgtctgtgcc tccctgaaga gcgcccagcc
     4861 gctgtcatag ggagtctccc ttgattccat gaagtttggc agctggacca ccaggaacgg
     4921 cacatcgccc tggccgaggc tgttcctcca gttccggatc attgccggca ggaggctcct
     4981 gtactcttca ggtcttcctg tgttggactc gccctggtac cacaggatgc ctttgatgct
     5041 gtaattcagc agtggagcca gcatagcgtt ataaagccct gccggcttat aaccaaaaaa
     5101 tgtctgtcct ctcagcggtg gcagaacagc ccctaccttg tatttccact caccctcgag
     5161 gctggttttg aagtcaccgg ccgtcagttc ataagtcttg tcagggacga agccggcatc
     5221 accgatattg cttattaatc tcaccgtcag aatgtttttc ccctctttca ctatacctgc
     5281 cggtactgtg taccatctcg gaggatattg gtaggatact gtcccgataa atgtgccgtt
     5341 cagaaatgca gaatccgcat ccacgatacg cccaaggttg accgttcccg gctggccggc
     5401 tgcagatgca ggtatgtcaa tctcttttct gaaccatacc acgccgttga tccctttcag
     5461 aggtgttgcc gaaaagaatc ccggcacagt aaaactgctc cagtcatcac tatcagggtc
     5521 gatatcgctc catctcagac ccccagccct gtatgccagg tcttccttct gaagattgct
     5581 gtaccaatca gcaatccgcc gccggtcttc cttttctata ttgcttatat aggactcttc
     5641 gttcagtttc cggagttcag catagtgttc aggaaatgcc tcaaggctct cttcgcttat
     5701 ccatgcttct gccggcgagc ctccgaaggc tgatagcaga attcctaccg gcaccccgca
     5761 ggtttgattt atctctctgg caaagaacca tgctgcggag gaaaaatcaa ggaccgtctc
     5821 cgggttggca gctctccatt cgcctcccgg caggtcactc tccggccctg tgaaaacaaa
     5881 tctctttggc actgtaaacg acctgattga gttgttctct gccgctgcaa tctcggcctc
     5941 gtagagtggc cttacgcgtc tgaccggcag ttccatgttc gactgcccgg agcacagcca
     6001 tacatcgcca atcaaaatat tgctgacagt cagcttgttg cttgccacaa tggtcattgt
     6061 atgaggccct ccggcctgca ttggcagcag cgttaccatc cattcaccgg aagaatcagt
     6121 ggtgtttgtg tattgtttac cagcaaattc tactgtaacc ttttctccgg gtgcagccca
     6181 gccccatatc ttcagttcag tatcgcgctg cagcaccatg ccgtcgctta tcagccgtgg
     6241 caaccgcacc tggctgttca gtggaatggt gatgagactt gcaaacagta ttgcggaaag
     6301 gatatcaagt tttgttttca taataatatg agacgtttga gtttgctgtt atacaatgct
     6361 ttatgccctg cggggcgata tcatgaatct tgtctttctt ttacatttct acatgtcgtg
     6421 gcgggttata attgccaata tcattgccct cactgcagtc cggttctttc actaaaatga
     6481 gccgccgggt catgttttca cgtctgctcc tctgctgctt tttgcttccg gtccctctca
     6541 cctggaatag ctctccggcg ggccaaggta tgatgatctg agaccgccgg tgtttaccac
     6601 aaccttttcc agtaccattc ccgggtcaat catccacacc ttcagggtat ggtagccggg
     6661 cccggctacc atgattttgc cctttacgta gcggatgctg ttgcggactg tctcttccca
     6721 ttccctgttg aaattctcgg cgttgaatcc cgcggggaca accgttatca gttgcggttc
     6781 gctgtcatca actgacagcc ctattctgaa gttcctgtca ggcaaaaagt tgagtgaagg
     6841 agccatatag agcactgttt caaactcgcc ggcagagaag aagtatatcc tgtactccag
     6901 cacaggtgag tctttgccgg gcactgcagg aggcgcatcc gtaaatgccc gggcacgcat
     6961 gcccgagcct gtcctgccgt aatcctcaat ctgctcccag cctctctcac cgctgtcatg
     7021 tctgccggta tagctgactg cctccattga tacgtagccg tcagcctcaa cgaatccctc
     7081 aacagtctca ggatcgggct cctccggccg gaaggcagta atacctattt taaattcact
     7141 cctttcagca gcgacggtta ctgttccgtt gtgctgcccg taaggaagtt ccggccagtt
     7201 gatggagacg tcaactcttt cctggtcagt gacggtgcca tggctgctgc tgaggatgat
     7261 ccaggggtga tcagcggaag cggtgtaaac gaacggctgg tctcccctgt tgaagagttc
     7321 aaaatatctt gtctgtttgt tgaaaacatc aaacgggggc aggaggggag ttgtcgctga
     7381 tcccggccat gcttccgcag aaccctctgc cgttatggca ggtaatgctc cttgggcggg
     7441 gaccggccgt tcaagcctta tatgtctgag gctgttcaca ggcggatcat tccaggcgat
     7501 atatcccagg tgggcctggt ccataaagtg tttccacctg ccgcctgcat aaacacggtt
     7561 atagtatccc atcagggcag tgtcggcagc aaagagctgc tctgtgcggt cagccatctt
     7621 cccggcattc gccctgccct gtgaagcgta cagggcattc ttcccggcgg tgacatagag
     7681 ctcatttacc agggcacatg cctttacagg gaagagaaca agatgaaagt aggcatcctt
     7741 catctctgca ggcaacagat gtccgatatg ctcagccttg tctgccagtc tgttatactc
     7801 ttccacaacc cgctcagcct catggtagtc aacaagagag taggttgaag gggaaagaag
     7861 ctcaggtttg cgacggccgt tgaacatggt gtaatctctt attatttctg agatttcgct
     7921 ggcatgctcc ggaccaaaaa catctgcagc ccattgctca gtccatgccg acatcccttt
     7981 actgcccatg gcgccggtat cccaggccag gtcaagaaaa tactccaaag ggatttcgta
     8041 tcccctgaag tgtcccacat tgacaatcca tatcctgtcg gcaccatact gttttgccag
     8101 tgacatctgg tcccatatct tcgggatggg acttgtgttt atccattggt agctgcgggg
     8161 tccgccgtgg taatcaaaat gatagtaaat cccggctccg ccgctgcgtt ttctttcatt
     8221 ggcaacaggg agacgccgta cgttgcccca gttgtcctct gcccacaaca gggtcacatc
     8281 gtcgggtacc ctcatcccct catgataata gtcctgtacc tccttataga gacaccatga
     8341 ctggggaacc tttgtgacat cactgtctat ctcctccgca atcatctcac gctggacctc
     8401 aactattttt tccagctttt cgatattggc cgcaggtccg ccgggtccca tttccgtatc
     8461 gtcagcgcca cgcagcccga tggttattat gctttcataa tctttgttgc gccgtattcc
     8521 ttcacgccag aatttcacaa gtgtgtcttc gtgatgtgtc cagctccagt ttccaagggt
     8581 tttcaggtac cgccggtccc attccttctg ggccctgatc atcggttcct ggtgactggt
     8641 acccatgaca atgccatact cgtcagccag gcttgcgttg cgaaggtcgt cttcattgaa
     8701 ggcattgttc cacattgcag gccagagata gtttgccttc agcctgagca gcagttcgaa
     8761 gatccgtgaa tagaactcac ttccataatt ggccacatca ggaggtaccg gcggattgac
     8821 agaaggtgtg acgtttccgt atttgaatgc aacccatctt gtcagtgccg ggtactcatc
     8881 attaaggaat atgccgcggt attttacaga gggttcgccc catacatgac gccccggaat
     8941 aacatataat tcactgcttt tacctgccgg cacgtctgcc caccagtgcc atggagaaac
     9001 accggcctgc cttgatattt cgtagatgcc gtagatggtg ccccgtttgt tgctgccggc
     9061 aatgacaagt gcactcttca ctcccgggaa gggtttttca acagtctgga taataaaaga
     9121 ttcccacttg tcttttatgg cgctgacatc aatcttcttc cttttggtca ggcggtctat
     9181 ggcggcgctt ttacctatcg ttcctgctat aatcatgaat tcagcccggg gcggttttga
     9241 ggctgtgatc agatccggca tcgcgcctgt taccaggctg aggtcattct gcaggtcggc
     9301 aagagccctt gtcactcccg gccactcccc ggggctgacg acaagcgggg cgcagctgcc
     9361 ggctgaggca aggataatgg cttcagctct cttcccggca gaaatgatca cctttgcctg
     9421 cttctgggcg gcagcagaaa aggagcagag taaaagcgcg accagcagag aggcgacacg
     9481 ggctccgggg tctgaaggtt gcgacccctt tcgggaatca ggatttcggc cccgccgccg
     9541 gctcctcagt ctggaagttg aaagtttgaa agtcaaaagt ttgaaagtca gaggtcggaa
     9601 gatcaaaggt ccgaagtcca gagatccgaa gttcaggagt tctgaagatc tggcagccgg
     9661 agggtcccgg cgacgggttg cagggtaatt attcatcagc agaaatcttg atttgtttac
     9721 agaagatcaa gaacgacagt ggttactgat ttggacttca aggtaatata cttgtcactg
     9781 ctgagaggct ggatctccct gtagttgttt attccgtcag tctggaatgc acggattgat
     9841 gttattgtcc tgcctgttac ttcgaatttg tagttgaccg catcaatagt cggattgacc
     9901 gccacaaccg taatcttcct gcctgaagga tcaatgaatg ctgacatcgg aaccgactgg
     9961 gacgatactt taaccctgaa ataacctctg tcaatctcct ttgaaaaatg cttcatcatg
    10021 taatagaaag gagtgacagt aaaagtgcct gatggagtca gcctgatcat ggagtcatta
    10081 ttggagtcgt cccacgccat cagccagtag atataacctg cggcatttgc ctcgttgatg
    10141 ttctggatga tgaaccatgc tgtgttgaac catgtgaagt ttgaatactc ggtcattatg
    10201 cagggtttgt ttccgtagga gtcacggatc atgttgagat cagcctttgt ctgggcaatg
    10261 agggtagaag cagtataatt gtacgtatga tatgcatagg cttccaggtg cggtttgttt
    10321 ttgagaggat ctgaaaaaac ggcgaaagtg tttcccccca gcttaccgct gtaacctatg
    10381 ttttcggcct cgggtccgat catcttcggc ggatcggttc ttgtcatgat cttgttgtaa
    10441 acagcgtcga aagctgcatc ataaccggca aattccgttg tctccacagg cctccattcg
    10501 cacgtttccc agtcgggcgt gatccaaccc ggttcattct gaatgctgat gtaatcagga
    10561 ttgaactgca ggttgtcaag aaggtcattc cagtactccc cgaaagcctc atacacaaac
    10621 gctcccttat cctttttgag ggtgccttcg cgcagattgc cgttgctctt cagcgccgac
    10681 gggggagtcc atgaactcac gagtgtctgt attgccggat tgagttgctt tgctatggca
    10741 tagagatctc cggtcacact ccacagcgtc tttattgaac cgttctccat ggtggcaggg
    10801 ctttttacgg ccgggtaacc aagcggatag taattattct tgaacctgat tatatccgtt
    10861 cccagatcct cgaaaagaag agtgcatatc tgctccttat gcgggcttga agtgatcctg
    10921 tcagagtacc aggtcagtga tcctccgaat ccgatcatct cctggtaagt caccgcaggg
    10981 tcaatggcaa tattagttat aaccggatca atatcaggct tttccggaac ttcgggttcg
    11041 ctgcacgact ggtacagcag aaccattcct gttgtaatta caaggagtat cgctcttttc
    11101 ataaaatcag aattcactaa atctgcggac catttcaaag aaaaggtccc gtaccagtcc
    11161 ctgttccggg ctcttaaaca acggtcagca tcgtcttctg cagatcctca tcccttgaag
    11221 aagggccggt cattatctca aaatcacccg gttccactac atatttcata tcaatattgt
    11281 ggaatgcaag tttgtcagga gtgatttcga aggtgacctt ctttgtctca ccgggcttca
    11341 acgagatccg cgcaaacccc tttagttcct tgacaggacg ggtcacactg ctgaccatat
    11401 cgcgtatgta aagctgcacg atctcatcac cggccacttt accggcgttc ttcacctcaa
    11461 ccatgacctt cgtggcttca ctcttcttta tagtcgtctt cttcagaacc ggcttgccgt
    11521 atttgaattt tgtatagctc agcccgtaac cgaagtggaa caatggtttg atatccaatg
    11581 aaatgtagtc ctgccaccgg gcgctgggct tgtggttgta atacatcggg atctgtccga
    11641 ccgatttcgg gaaggtgatg gtaagcttgc cggacggatt gacctctccg aagatgatgt
    11701 ctgcggctgc agtacctgtc tcctgcccca tgtaccatcc ctcaacaata gcgggaactg
    11761 tttcagccag cttattgatg gagagcggtc tgccgttagc caggtaaagc accacgggct
    11821 tgccgagggc tgttatggcg tctgccagtt cattctgttc gccgaagaga tcaagggtca
    11881 tgttgtcacc aatatggttc tgagcccatg cctcgcggca gagatgttca ttttcgccca
    11941 gggcaagtat taccaggtca gcttcctttg ccactttcac ggcttccttt atgagtttga
    12001 gattctcttc acgcgaagcg aagatgatct catcatattt ccagttgaaa tagctgagcg
    12061 tagtgttgat ggtcagcctg caaccctgtg cgaagagcac ttcggtgccg cttcctgcct
    12121 tcgccctgat tccgtcaagg agactcacct tctcataagg ctcgcccgaa tagcctccga
    12181 accacacatc cttcgcgcag gggccgatga cagcaatctt tttgtatttc cccttcttca
    12241 gcgggagaag cccatcgttc ttcagcagga caatagactc atgggcggcc ttcagtgctg
    12301 tcttcctgtg catcagtgat ttacttaccg ctacggcctc tttctcatct atatatggat
    12361 tctcgaagag ccccatgctg aacttgaatg ccagcgcctg ctcaacagca tggtcaacat
    12421 cttccttttt gactttcctt ttccttacca gttcgggaag gaggcgatac aggtttgcct
    12481 gcgggaactc gaattgcacc ccggagttga atgctgtgat ggttgcatcc acagcatcgg
    12541 tacagacaaa gtgcttgtta aagagatgtt ctacgccatt gtagtccgat acaatcattc
    12601 ccttatagcc ccattcatcg cgaaggagat ctttcagaag ccacctgttc gcgtgcgagg
    12661 ggactccgtc gacctcgttg tacgaaggca tgattgacac cggttttgca tggtcaatgc
    12721 acatcttgaa cggcggcatg tgataatcac gcagcaccct tattgaatgg ttggcaggcg
    12781 cctggttgag tccgccttca gattcgccat ggcccacaag gtgcttgagt gtagcagcaa
    12841 cattgttctt gtcaactttg ccgttgtcac ttccctgcag tccctttact gctgctattg
    12901 agagcatccc gttgaggtac gggtcttctc cataggtctc ctcggtgcgc ccccagcgcg
    12961 ggtcgcggca gacatcaata accggggtaa gtgcgtgatg ggtaccacgc gatctcattt
    13021 catgggctac aatgccgtac actttttcaa agagcgcggt atcccaggag catgccagcc
    13081 ctattgcctg cgggaatacg gtggcttccg gcctcatcat gccatgctgc ccctcgtcaa
    13141 caaagagggc ggggataccc aggcgggtct cttcaaccag atacttctgg atggcattgc
    13201 gcatcgtgac ggtgtcttta ataccaaagg gagccggatg aaccgagtgg caaccgagac
    13261 caaagatctc cttcatcttt gcaggatcac tgaagatctc aggtttgaaa tcctctactc
    13321 ctccattcca gagtcccatc agctgggcac atttctcctc aagagtcatt cttcccatca
    13381 ggtcctcaac tcttttcttt atggaaagag aaggatcctt atacggtgca tttactgttt
    13441 ttttcattat gatgtgattt ttatttaagt tttttcacct cccggatgac agcatcatag
    13501 gctggtttgg gagtatagtc acggttgaaa agaagcgggt agttggtacg gccggggatg
    13561 ggccatccgt tcttccatga atagccgtca tgtacgcccc agaaggtcac cctggtaatg
    13621 agatcgtcat gcttcagtag tatcctgaaa acctcttcat agcgatttgt aagtagcatc
    13681 tgaacggaat cgggaagccc ttccacgtag ggattgagtg agtcaaggta cgcacccatg
    13741 ttagatatct ctgccccgaa aaaccttgta ggagtaggca gcacgttgat ttccatctcg
    13801 gtgatcatca cctttatccc cagtgcgcca tacaggccga tgctctcgtc gatgattgcc
    13861 aggtcaggtg agtcgaggtg ccagtgcccc tggattccca caccgtcgat acgaatccct
    13921 tttgcctgaa ggttctctat tattggtatc accccggcaa gtttggcagg ctcctcaatg
    13981 ttgtagtcat tgtaatagag ttctgcatca gggtcagcct cgttggcgaa gatgaaagcc
    14041 ttttcgatgt agtcttcacc tattatttcg agccattttg tattacgcag cgtgccatct
    14101 tcgttcaagg cttcgttgac cacgtcccag ccgaggacac ggcctttgta gtgtccgacg
    14161 acggtatgga tatgctcacg catcctttcc aggagagcat cgcgtgtcag ctgattgcca
    14221 agtgaatcct ggaaaaccca cctgggcgtc tgggagtgcc ataccagtgt atgaccgatg
    14281 actttcatgc cgttctgttc cgcaaagtcg acaatactgt cggccaggcc gaagttgtaa
    14341 acacccggtt cgggatggat acgttcccat ttcatttcat tctcagcggt gacggagttg
    14401 aactgactgg caatgaaagg cagggcctgc ggatcagtac cggagatctg catcccgttc
    14461 atggcagtgc ctatcaggaa gtgatcttca aacgccttct tcagggtgat gtcattgttt
    14521 ccgcatgctg agagcatgat cgcaatggct gcaacagccg gcataaaaat ctttttcata
    14581 gttgttttat ctgtttataa ttcataatat ctgcatctgc cagttggttt attccgtata
    14641 ggctttgcgc ctttctgcca gttcatcctg catctggtat tcaacctttt tcccgatgtc
    14701 gtatctgaag agcgctacaa cgcagaagac cagtgcgatg accgggaata tgctcaccga
    14761 gagtctgatc ccctgtacgg ctgttgctgt ctgtgccacg ttggcaacat atccgtatcc
    14821 attaagcagc catccggcca atgcaccgcc gacagccagc ccgacttt
//
LOCUS       DAOPBK010000050        14857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4073_length_14857_cov_5.114917, whole genome shotgun sequence.
ACCESSION   DAOPBK010000050 DAOPBK010000000
VERSION     DAOPBK010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14857
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4073_length_14857_cov_5.114917"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3297)
                     /locus_tag="PLA17_04430"
     CDS             complement(<1..3297)
                     /locus_tag="PLA17_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TANFOR domain-containing protein"
                     /protein_id="HPK84694.1"
                     /translation="MIVFLSLATFCNAQAQTTMSVSVMPPYSNKLSDYTATPGKLNVI
                     INSPYGLDGMDVEYYLLGTIINSDESIIIRTRRDYKPPQPMVIKVVQSPGGAFITPPY
                     VLTYNDIRQIFDEQNLEYSGITRHEVMQNGLPEGIYTICFDMIHYTYSQNIIAACSAP
                     FSISSVEAPIIISPLNNTTLREPETKNVVFTWTRPPNAPVNTQYKLKIIELNSPTDNF
                     QDKIRSAGYPTFFETTVNGNAYLYSAANPALKPGKTYAFVVSAIDPSGGTAFRNKGFS
                     EINLFTYQKPDGQQTMIGKMETKPGFIQANKEIITSMLRPTTLKGTLVYQFKNGGEKK
                     PLSGARIKLVEKYIAKNADGTVNMVTTHRINKNPDYMSKDIKTVTTGDNGEFSFTFLS
                     NNKGEPLAETDCYVIVAKLFNHPSDDQNNNLFADLSNTMQRGGPNPGNMTLENSATDN
                     NSCKIYRCYGIEIEGEHARYYLNPDQELRYFFEVNGGDTKDAGEVVSLVKTIDLNVRV
                     EAKDAGKFTINNKKELANMNVYIYRKVNFDYPSLFPENDVTPDKTDNFPPPKGNGMIC
                     VGKGLTDENGIARIKSLVLSDNPTYQYFMYIRMDMGNYNYESKEMTPIDFKELVEGNT
                     TYDGKTTFIPNLSLQDLLSFHSGGEFYTLNNDTRLIEGFDLTQQMELKFPTLRVIITE
                     KDGNKQIDDPQTRIILEEKYSEGTLIIEPGNVGQPLQSPLTVTKTMAQIDGGRYEINN
                     LPVASQVYPAEIKGPSRKITIKAPGFDEVTLTVKEGNPLYFSERYEMPVLLNYGAKFK
                     GTVFDAETNKPLDNVFLNILGETSKSTSTGPDGKYYTEVKKLDTDRTVVITREGYMSD
                     TVVLKLNKSFNGHNFVLYRKARRLRVRVWSTTSSSNKKGFVVTLPNVPSSWRVEPNTI
                     NNTALQITTGSVAKQMASIVSAVSSTGSGSSKPASSRTLQRTSSAGMVQMVSQSSSPY
                     TVTTDENGVADFMFTGGGDENQKFEVQVTNSPTSSENIESAIVNVEIPVQDYFVNKTV
                     TLLQGGCLSGMVYLGEGTEQPVSDMVVEATYRADDGYYTVKAVTDASGKFLLRNLPGY
                     SKQFKLQITAG"
     gene            complement(3320..4966)
                     /locus_tag="PLA17_04435"
     CDS             complement(3320..4966)
                     /locus_tag="PLA17_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84695.1"
                     /translation="MFVISTLNAQDLANLKGTKPVLVNGSIGFASSFYNASGIPDRQS
                     PFAYGINANATLTLYGISMPFSFTWYSNDKAGFRQPFNQFGISPTYKWLTVHLGYRNV
                     SFSEFTLNGFTFLGAGVEARPGKFRLGAVYGKFNQNSSYDLVMADSIPKLTRMGWATK
                     VGYGTDDRFVDISMLRIGDNPKNFVDSLAKLGQPTPAQNLAVGLASKFRISKNLFFHF
                     DASMSFFTQNQKIAASDSITDGLLKFAGNFITVNNTSEYHKALKTGLSYRFTPTVISG
                     IEYRRIDPGFQSMGSYFFNNDLELITLNQSASFIKNKLNARGSLGLQRDNLGSTKNST
                     SKRVIGSLSMNYNINQNWGVDASYSNFSTNQKAIKNVTDNTLMIYQVNHNLMFMPRFV
                     KAGEKFSHMVMLNLNWMILDDKNTQTSSATDTDTKVAMLMYALGLLKQKININVGVSY
                     TNMSNQSLSNQLIGSTLGVSSTLLKDKLLLNWNNSFMLNKINSDNGTVFNTGLSANYR
                     FLPKHTVTLTFNLINNSFSDSATVPSFNEFRGDIGYVFTF"
     gene            complement(5344..5430)
                     /locus_tag="PLA17_04440"
     tRNA            complement(5344..5430)
                     /locus_tag="PLA17_04440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5394..5396),aa:Ser,seq:cga)
     gene            complement(6008..6388)
                     /locus_tag="PLA17_04445"
     CDS             complement(6008..6388)
                     /locus_tag="PLA17_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPK84696.1"
                     /translation="MKRRLPGIILSSITCCLLIMSLSTAAGQTGDGQPAQARSAVIDS
                     VAHSLAERIKVYPNPVRSELIADHIEGVTMIEIFDVTGNKCLNEVCDNHNQMTIEVST
                     LKKGIYFIRFISPGATIMKRFIKE"
     gene            6738..8831
                     /locus_tag="PLA17_04450"
     CDS             6738..8831
                     /locus_tag="PLA17_04450"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPK84697.1"
                     /translation="MRRAFILIMAVALAAVSCNTQTKDQGNPFYAEWKTPFGVPPFDQ
                     IRNEHYLPAIDSGIALARLQIAEITANEDAPTFANTVAQYDRAGELMSKVAYVFYSQT
                     SANTNDTLEALQMEIAPKMSAFSDEVLLNAELFSRIKVVWENRSAEELNDEELFLLEN
                     MYKSFVRNGALLGPEDQEILKKLNQELSLLTVKFSQNVLEETNSFRLVIDDEADLKGL
                     PESVIAGAADEAKSAGMEGKWVFTTRKPSMIPFLQYADNRDLRKKLYDAYLNRGNNGN
                     ENDNNKILADIVRIRAERAKLLGYRTHADIVLETRMAKTPENVLNLLNNLLERSLRVA
                     KRERDEMQKIADATGGNFKLEPHDWWYYAEKLRKARYDLDENELRPYFSLDNVRDGAF
                     AVANKLYGITFSPIDKIPLPHPDAQAFEVKEADGSHIGVLYMDFYPRESKRQGAWCGG
                     YRDHKIVDGREITPVVTIVGNFTPPSGGTPALLSMDDVLTLFHEFGHGLQALFSENTY
                     SSTTVAWDIVELPSQIMEHWATKPEVLAMYAKHHETGEIIPEALVEKIRNARYFNTGF
                     DNVEIMAASLLDMAYYSLEAPVQVDPQQFEKETMKKIGLIPEIEPRYRSTYFLHIIDG
                     YDAGYYVYTWAAVLDHDAFEAFNENGLFDQATATSFRKNVLEKMGIMDAEKMYLNFRG
                     REPDIEPLLRNRGLN"
     gene            complement(8893..11118)
                     /locus_tag="PLA17_04455"
     CDS             complement(8893..11118)
                     /locus_tag="PLA17_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPK84698.1"
                     /translation="MEATAKKSGFGTAPVYFTAISTIIGAIVFLRFGFAVGTLGFWGT
                     LLLIIMGHAVTIPTALAISELATNKRVEGGGEYFIISRSFGLNIGATIGIALYLSQAI
                     SVAFYIIAFAESFEFLFDFVAQRFSFILPRQVISLPALVILGLMILRKGSATGVKMLY
                     VVNAILFITLLLFFLGKPAEGSLATGQLPLRDIRNMSDFFIVFAICFPAFTGITAGVG
                     LSGDLRNPARAIPLGTILATLTGMVVYILVIWKLAISASPEELLADQLIMGKIAIGGA
                     IIIPLGLASSTLTSALGSVMVAPRTLQALAKDTSFPSMRLNRWLASARSHDGEPVNAT
                     LVTLLIASLFVLLGSVNAVAEIISMFFLVTYGSLCLISFLNHFGSSPSYRPSFRSKWY
                     LSLTGCVIAGIVMFKINTLYALTASVLIVIIYSCINHYHKDRTGLESLFANTLFQINR
                     NLQVYLQKSGSRRKEKEWRPGVICISKDSFRRERAFRLLNWLSYSYGFGTYLHRIEGY
                     YSRATSQQAAEELAKLIELYETTHNHVYVDTIISPSYTSAIVQAIQVPGISGMENNMV
                     VFEYDKDDPENLPEIIDNIRLIKAGNFDLCLLASSSREMICSKGIHVWIDFNEPENAS
                     LLILLSFIISGHPDWKKSFIKVFSTCKPGQVEATRRELEALITSGRLPITEKNVEIIE
                     ITHGSSVKSLINEKSATAAMVMTGFRPEMLRQNGEAILGGYDEAGTILFVNSQYQKEI
                     I"
     gene            11219..12253
                     /gene="rlmN"
                     /locus_tag="PLA17_04460"
     CDS             11219..12253
                     /gene="rlmN"
                     /locus_tag="PLA17_04460"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPK84699.1"
                     /translation="MSREKETLAGSDLEESEELLGRLGIDRRHSRRLLYWVYRRGIRS
                     FDEINDIPQKFLRILSEYAVPGLSSPTASEVSSDGSVKYLFTSKNGMAHETVFLPDDK
                     RRTVCVSVQSGCRMGCRFCATGKSGWNGNLTAGEIVNQVISIPHEITHIVMMGMGEPG
                     DNIDEVIRACRILTAEWGLAAGRRRVTVSTVGVTPSVRRLLDETECNITLSLHSPFPE
                     ERLEVIPAEARWPFAGTLDMLKQYDNRIKRRFTVAYVMIEGRNDSQRHLEELKRLLAC
                     TGIRVNLLPYHSVDEEDERSSGISVMMQFKHELVTSGIGASVRKSRGADIAAACGMLA
                     ARSRRGARKQ"
     gene            complement(12439..12969)
                     /locus_tag="PLA17_04465"
     CDS             complement(12439..12969)
                     /locus_tag="PLA17_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK84700.1"
                     /translation="MILENDGVRLRAVEPEDLELLYRWENDETNLRQGNTLVPYSRYI
                     LKKYIAESGKSIFETGQLRLMIDVLPDERTIGTIDLYEFDPFHNRAGVGILIADESDR
                     QKGYASAALACVIEYAFEIIGLHQLWCNILESNAPSLNLFTNHGFSICGTRKEWTRVS
                     DGYETEHLLQLIKPHK"
     gene            complement(12953..14599)
                     /locus_tag="PLA17_04470"
     CDS             complement(12953..14599)
                     /locus_tag="PLA17_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84701.1"
                     /translation="MGIRGISFLMLLLLLLPLNGQKKSELKEIWAEAESHYLYGEYEL
                     ANPLYLILNELIPGNSNIKYKIGNCYLNIFDEKQKAIPFLEEAVKNTSYNSKTGKLKE
                     TRAPLDAYFSLANAYLIANKLDSAMNTYRFFSQLITETSSMQNKEFINQQMQACNVAS
                     EQMNNPVRVRIQPLKGYINQGLVNERPVVSYDGNTMAYTERRGLESVVYVTRMEGTSW
                     GTPVDITLEASMGTDCHTTSLNNDGTELYLFKNDNYDGNIYMTRYTDGKWSHIEKLNK
                     NINTKFYESHAAISSDGKKLYFTSNREGGLGELDLWVSEKDQTGDWGVPTNLGNVVNT
                     PYNEETPFISGDGNTLTFSSEGHGSMGGYDIFLAKLSNNQWTTPVNIGYPVSSTDDNI
                     AFQPFDNGRQGFYSIMTGYKKKEIAHVDFNVPDEEKKDTVTISFNLDDLPYLTVVDSS
                     MLITDMVIRDVKDTDETVEPDVLYYTVQVMALYNPVDPAYFEYAKISVFYNSEDQFYR
                     YTTGRFDSKASAYEERDRLLSLGYPGDIFIKKVYRNDSGK"
     gene            complement(14658..>14857)
                     /locus_tag="PLA17_04475"
     CDS             complement(14658..>14857)
                     /locus_tag="PLA17_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84702.1"
                     /translation="GYDIFCSELQYNGLWSTPENIGYPLNTPDDNIFFCPTDNGKGGY
                     MSFSGRDDSSGGADIYRVRFK"
BASE COUNT         3786 a         3362 c         3587 g         4122 t
ORIGIN      
        1 tcccgctgta atttgcagtt tgaattgttt ggagtacccg ggcaaatttc gcaacaggaa
       61 ttttccactt gcatcagtca ctgctttaac ggtgtaatag ccgtcatcag cacgataggt
      121 agcttctacc accatatcgg aaacgggttg ttcagttcct tcacccagat aaaccatgcc
      181 cgaaagacaa ccaccctgaa gcagggtaac cgttttattc acaaagtagt cctgaacagg
      241 aatctccaca ttgactattg ccgattcaat attttcggaa gaggtgggtg agttagtcac
      301 ctgcacttcg aatttttgat tttcatcgcc tccaccggta aacataaaat cggcaacgcc
      361 gttttcatcc gtggtgaccg tgtagggaga ggagctctga gataccatct gaaccattcc
      421 ggcactgctt gttctttgta aagttcttga tgaagcaggt tttgaggagc ctgatcccgt
      481 tgaggaaaca gcagagacaa tagaagccat ctgttttgca acactgcccg tggtaatctg
      541 caatgctgta ttattaattg tgttcggttc aactctccac gatgagggta cattgggcaa
      601 cgtaacgacg aatcctttct tgttgctgct gctcgttgtt gaccagactc tcactctcaa
      661 tcttcttgcc ttcctataga gtacaaaatt gtgcccgttg aatgatttgt tcagtttcag
      721 gacaacagta tcggacatat atccttcacg tgtgataacc accgtacggt ctgtatccag
      781 tttttttact tccgtataat attttccgtc aggcccggta gatgtggatt tggaggtttc
      841 gcccaggata ttcagaaata cgttatccaa tggcttattg gtttcggcat caaatacagt
      901 tcctttgaat ttggcaccgt aattcagcaa gacaggcatc tcatatctct cactgaaata
      961 gagcgggtta ccctctttga cagttagcgt aacctcgtca aatccgggtg cttttattgt
     1021 tatttttcgc gaaggcccct tgatctctgc cggataaacc tgggaggcta ccggcaaatt
     1081 gtttatttca tacctgccac cgtctatttg agccatcgtt ttcgttactg tcaggggcga
     1141 ttgtaatggt tgccccacat tccccggttc gataatcaga gttccctcag aatacttttc
     1201 ttctaaaatg attcttgtct gcggatcatc tatttgtttg tttccgtctt tctctgtaat
     1261 aataacacgg agggtgggga attttaattc catctgttgg gtgaggtcaa aaccctctat
     1321 gagtcgggta tcattattta atgtgtaaaa ctcaccacct gaatggaagg aaagtaaatc
     1381 ctgcaaagat aagttgggaa taaatgtagt ttttccgtca tacgtagtat tgccctccac
     1441 taattccttg aaatcaatgg gtgtcatttc tttcgactcg tagttgtagt ttcccatgtc
     1501 catcctgatg tacataaagt actgatatgt aggattgtcg ctaagcacta acgattttat
     1561 ccttgctatt ccattttcgt cagtcaaacc tttgcccacg caaatcattc cgttgccttt
     1621 tggcggcggg aaattgtctg ttttatcagg agtaacgtca ttttccggaa agagagaggg
     1681 ataatcgaaa ttgactttcc gataaatata gacattcata ttcgcgagtt ctttcttatt
     1741 gtttatagtg aacttaccag cgtctttcgc ttccaccctg acattcaagt caatcgtttt
     1801 aaccaatgaa accacttctc ccgcatcttt tgtgtcgcct ccattcactt caaaaaagta
     1861 tctcaattcc tgatcgggat tcaggtagta gcgtgcgtgt tcgccttcaa tctcaatacc
     1921 gtagcaacga tatattttac aggaattatt atcagttgca ctgttttcca gggtcatatt
     1981 tcccggatta ggacctcctc gctgcattgt gttagagagg tctgcgaaca gattgttatt
     2041 ttgatcatcg gatggatgat taaacagttt ggcaactata acatagcaat ccgtttctgc
     2101 caatggttct cctttgttat tggataaaaa agtgaaagaa aactcgccat tgtctcctgt
     2161 ggtaactgtc ttaatatctt tactcatata atcaggattt ttattaatcc tgtgagtcgt
     2221 aaccatattt accgttccat ctgcattttt tgcaatatat ttttccacca gttttattct
     2281 ggctccgctc agcggttttt tttcaccacc atttttaaac tgatagacaa gtgtgccttt
     2341 cagtgtggtg ggccggagca ttgaagttat gatctctttg ttggcctgaa tgaatccagg
     2401 ctttgtttcc atcttcccga tcatcgtttg ttgtccatcg ggcttttggt aggtaaaaag
     2461 gtttatttca ctgaatcctt tgttccggaa agcagtccct cccgacgggt caatggcgga
     2521 aaccacaaaa gcataggtct tgcccggctt gagggcggga ttggcggcag aatataaata
     2581 ggcattgcca ttcacggtag tttcgaagaa cgtaggataa cctgccgaac ggattttgtc
     2641 ctgaaaatta tcagtcggac tgttcagttc gatgattttc agtttgtact gcgtattcac
     2701 cggtgcattg ggcggacgag tccacgtgaa taccacgttt ttggtctccg gttcgcgcag
     2761 tgtcgtattg ttcagcgggc tgatgatgat gggagcttcc accgaagaga tggaaaacgg
     2821 agcactacag gcagctataa tattctgact gtacgtataa tgaatcatat caaaacagat
     2881 ggtatagatt ccttcaggca agccgttttg cattacttca tgacgtgtga tgcctgaata
     2941 ttcaagattt tgttcatcga atatctgacg tatatcgttg tatgtaagta cataaggcgg
     3001 tgtaataaat gcgccgccag gcgattggac tacctttatt accatcggtt gcggcggttt
     3061 ataatctcgc cttgtgcgga taataatgga ttcgtcggag tttatgatag tcccaagcag
     3121 ataatactca acatccatcc cgtccaaacc atacggagag ttaatgatca cattcaattt
     3181 accgggagtt gcagtgtaat ccgacagttt gtttgaatag ggcggcatca ccgagactga
     3241 catggtggtc tgcgcctgcg cattgcaaaa cgttgccagg ctcaggaaaa cgatcaatga
     3301 tcgtttgatt ctttttattt tagaatgtaa acacataacc aatatctcct ctaaattcgt
     3361 taaatgatgg tactgtggca gaatctgaaa aactattgtt aatcagatta aaagtgagtg
     3421 ttaccgtatg tttcggcaga aagcggtaat ttgcgctcag tccggtattg aaaaccgttc
     3481 cgttatcgct gtttatctta ttgagcataa aggaattgtt ccaattgagt aaaagtttat
     3541 ctttcaacag ggtggacgaa acgcccagcg tacttcctat tagttgattg gaaaggctct
     3601 gattggacat attggtgtaa cttacgccca cgttgatatt tattttttgt ttcagcaaac
     3661 ccagggcgta catcaacatc gccactttgg tatcggtgtc ggtagcgctt gaagtttgcg
     3721 tgtttttatc atcaagtatc atccagttca gattcaacat taccatgtgc gaaaactttt
     3781 cgcctgcttt aacgaatcgg ggcataaaca tcagattatg atttacctga taaatcatca
     3841 gcgtgttgtc tgtcacgttt ttaatggctt tctgattggt cgaaaaattg ctgtacgaag
     3901 catcaactcc ccagttttga ttgatattgt agttcatgga aagtgagccg ataacgcgtt
     3961 tcgaagtact atttttggtg ctgccgaggt tgtctcgctg caatccgagt gacccgcgtg
     4021 cattcagttt gtttttaata aacgaagccg actgattgag cgtgataagt tccaggtcgt
     4081 tgttgaaaaa atagcttccc atggattgga agcccggatc tatcctgcgg tactcgattc
     4141 cggaaatcac ggttggtgtg aaacggtagc ttaagcctgt tttaagggct ttgtggtatt
     4201 cgctggtgtt gttcacggta ataaaatttc cggcgaattt aagcagcccg tccgtaatgg
     4261 agtccgaagc cgctattttc tggttttggg tgaaaaaact catcgaagca tcgaaatgaa
     4321 agaacagatt ctttgaaatc ctgaactttg aagccagtcc aaccgccagg ttttgtgcgg
     4381 gtgtaggttg acctaatttt gccagactgt ccacgaagtt tttcggattg tcgccgatac
     4441 ggagcatgga aatatccaca aagcggtcgt cggttccgta acccactttt gttgcccatc
     4501 ccatgcgtgt tagtttggga atggaatcgg ccataacaag gtcgtaactg gagttttggt
     4561 taaatttacc gtacaccgca ccaagccgga atttcccggg acgtgcttcc acaccggctc
     4621 ccagaaaagt aaatccattt aacgtgaact ccgaaaacga tacgttccgg tatcccagat
     4681 gcaccgtgag ccatttgtac gttggactga tgccgaactg gttgaacggt tgccggaatc
     4741 cggctttgtc gttgctgtac caggtgaacg aaaacggcat gctgataccg taaagcgtta
     4801 acgtggcatt ggcgttgatg ccgtaagcaa agggtgattg gcggtcgggg atacccgagg
     4861 cgttgtaaaa agaacttgca aacccgatgc tgccgtttac cagcactggt ttggtcccct
     4921 tgagatttgc taaatcctgc gcattcaatg ttgatataac aaacattaaa atgctgaaaa
     4981 gcagggttct gttgagtttg tcatttaaca ttgcaattta ttttgtcagt taaatagcga
     5041 tatttcctgc tgaaacagaa gacagggtca ttctggaata aagatatgca actttatatc
     5101 tctaaaacta taaagtcacg gttattatta tctgtatcgt agtgtttggt ttaccagcag
     5161 gagcgcgctc acggctgcct gcaccaatat gttcatcgcg ctctggttcc atcaggcttt
     5221 gtaactcttt ctggggcttt caaatggctt tacacccgga cagtctcata tgcaaaagat
     5281 cctgcaagtc attgatttac aggacctttc ttgggtttgg ctcattctct cttagccttt
     5341 ccgcggagag agagggattc gaacccccgg tccggttacc cggacaacgg ttttcgagac
     5401 cgccccaatc gtccactctg gcatctctcc ggactggctt tgcccaccgg gcacagcgga
     5461 tgcaaatata actcaaattc ttaagactca tttttgttca tagaaaactt acacaagctc
     5521 tgtttgatgt cataacatag agtaggtgaa ggagccggta aagggtaccc cggagtaacc
     5581 ggcaaggcac catgaatatt ttttcggtaa agtgtaaact attccggatt ttatccgtct
     5641 ttatataaag agatgagaaa atcttctttg gttagtttca ggtaaacact aagagggtgt
     5701 cttataatac aaatgaaatg gggcaccctt tttctttttg tcaaagccgc atcgcatcag
     5761 cggttttttt cataaatccg gaacctgcaa tgcagcatac catagtcaac aaatccggaa
     5821 aggcgaagaa cagcaatgcc tcccggaata tcctttcatg cgaccggtga gcaaaaggcc
     5881 gcctgtaaca tctttcatgc gaccggtgag caaaaggccg cccgtaacat ccatcatgca
     5941 agctgtgaac aaaaaaaacc gcccggaaga tccatggcgg ttagtgtata aaaaaagaac
     6001 gtgccggtta ttcttttata aatctcttca ttatcgttgc tccgggagag atgaacctta
     6061 tgaaataaat ccccttcttg agcgtggaaa cctctatggt catctggttg tgattgtcgc
     6121 atacctcgtt aaggcattta ttcccggtta catcaaatat ctcaatcatc gtgacgcctt
     6181 caatatgatc ggcaatcagt tcactcctga ccgggttggg ataaaccttt attctttcag
     6241 cgagactgtg tgccacagaa tcgataacgg ctgacctggc ctgggctggc tgaccgtcac
     6301 ctgtttgtcc ggctgcagtg ctcagcgaca ttatgagcag gcagcaagtg attgatgaca
     6361 agataattcc gggtaaacgt cttttcatag gcaaattgtc aatacttttg cgaggttagt
     6421 cattattgca tcaaatttaa cagatgcaac tgcactttat actccttttc cgtcataaat
     6481 gtttcaaact ttaataagtt atcaacaaaa ttttttttca gagtcttcgt taagccccgg
     6541 atcatgcccg ggttccgggc gaggaatgaa aattcataga aggttttgtt taaaaaaact
     6601 acctttaagg tctgaattaa aaatcaaaaa taaaacttaa cgtgaaaccc acatgtgccg
     6661 gacagaaaaa catctgttta ttatggttta cagtaatgtg gattccatca tacctttaaa
     6721 accaaaatca tattatgatg agaagagcat ttatactcat tatggcagtt gcacttgcag
     6781 ctgtctcatg caacacacaa acgaaggatc agggcaatcc cttctatgct gaatggaaga
     6841 ccccctttgg cgttccaccc tttgaccaga tcaggaacga gcactatctt cctgccattg
     6901 actcgggcat tgcccttgcc aggctgcaga tagctgagat cactgcaaat gaagatgcac
     6961 ccacatttgc caacactgta gcccaatatg acagggcagg tgagctgatg tcgaaagtgg
     7021 catatgtgtt ctattctcag accagcgcaa acaccaatga tacccttgag gcgctgcaga
     7081 tggaaatagc tcccaaaatg tcagcattca gtgatgaggt tttactgaac gcagagcttt
     7141 tcagccgtat aaaggtggtt tgggaaaaca ggtcagctga agagctgaat gacgaggagc
     7201 ttttcctgct cgaaaacatg tataagagct ttgtgcgtaa cggcgccctc ctgggtccgg
     7261 aagatcagga gattcttaag aagctgaacc aggagctctc tctcctgacc gttaaattca
     7321 gccagaacgt cctggaggag accaacagct tcaggcttgt cattgatgac gaggctgacc
     7381 tgaaaggtct gcctgagagt gttattgcgg gtgctgccga cgaggcaaag agcgcaggca
     7441 tggagggcaa atgggttttc accacccgga aaccgagcat gattccgttc ctgcagtatg
     7501 ctgataaccg tgatctgcgc aagaaactct atgatgcata ccttaatcgc ggcaacaatg
     7561 gtaatgagaa tgacaacaac aagatattgg ctgacattgt aaggatcagg gcagagaggg
     7621 cgaagcttct gggatacagg acccacgctg acatagtcct tgagacacgc atggccaaaa
     7681 cccctgaaaa tgttctgaac ctgctcaaca acctgcttga gaggtcactc cgggttgcaa
     7741 agcgtgagcg tgatgagatg cagaagattg ctgatgccac tggcggcaat tttaagcttg
     7801 agccccatga ctggtggtac tatgcggaaa agttacgcaa agccaggtat gatcttgacg
     7861 agaacgaact gaggccgtat ttcagtcttg acaacgtacg tgatggcgcc tttgccgttg
     7921 ccaacaaact ctacggtatc accttctccc ccatagataa aattcccctt cctcaccccg
     7981 atgcacaggc atttgaggtg aaggaagccg atggcagcca tataggtgtg ctttacatgg
     8041 acttttatcc gcgtgagagc aagcgccagg gagcatggtg cggaggttac cgtgaccaca
     8101 agatagttga tggcagggag atcactccgg ttgtgacaat agtgggcaat ttcactcctc
     8161 cttcaggagg cacaccggcg ctgctgagca tggatgacgt actgactctc ttccatgagt
     8221 tcggtcatgg gcttcaggct cttttctccg agaataccta ctcatcaacc accgtggcat
     8281 gggatatagt tgaactgccc tcgcagatca tggagcactg ggccacgaag cccgaagtgc
     8341 tggcaatgta cgcgaagcat cacgaaacag gggagataat acctgaagca ttggttgaaa
     8401 agatcaggaa cgcaaggtac tttaatacag gatttgacaa tgttgagatt atggctgctt
     8461 ctcttcttga catggcctat tactcacttg aagctccggt gcaggttgat ccgcagcagt
     8521 tcgagaagga gaccatgaag aagattggtc tgatacccga gatcgagcca agataccgga
     8581 gcacctattt cctgcacatt attgacggct atgatgccgg ttattatgta tatacctggg
     8641 cagcagtgct tgaccatgac gcttttgagg cattcaacga gaatggcctg tttgatcagg
     8701 ccactgccac atcgttcagg aagaacgtgc ttgagaagat gggaatcatg gatgctgaaa
     8761 agatgtacct taacttccgc ggccgtgagc cggatataga accgttgctc aggaacaggg
     8821 gactgaactg accggcaggg ttcatcagga tattcaagag gctgccatca tgcgggaggc
     8881 agcctcttgc ttttatataa tctctttctg gtactgcgag ttgacaaaca ggatggtgcc
     8941 ggcttcatca tagccaccga ggatagcctc accgttctgc ctcagcatct ccggtctgaa
     9001 accggtcatc accatggcag cggtggcaga cttttcatta atgagacttt tgactgagct
     9061 cccgtgagtg atctcgatga tctccacatt cttttcagtt atcggaagcc ggcccgaagt
     9121 gatcagcgcc tccagttccc gtcgtgtcgc ctcaacctgg ccgggtttgc atgtgctgaa
     9181 taccttgatg aatgattttt tccagtcagg atgacctgag atgatgaagc tcagcagtat
     9241 cagcaggctt gcattctcag gctcgttgaa atcgatccat acgtgaatgc ccttgctgca
     9301 gatcatctcc cttgaggagg aggcaaggag gcataaatca aagttgcctg ccttgatgag
     9361 acgtatattg tcaatgatct ccggcaggtt ctcaggatca tccttgtcat attcaaagac
     9421 aaccatattg ttctccatgc cggagattcc cggtacctgt atggcctgga caatggccga
     9481 tgtgtacgag ggagatatga tggtgtcaac atagacgtgg ttatgcgtgg tctcatacaa
     9541 ttctatcagt ttagccagct cctccgctgc ctgctgactc gtagcccggg aatagtatcc
     9601 ttcaatccgg tgcaggtagg tgccgaaacc ataactgtaa gagagccagt tcagaagccg
     9661 gaaagccctt tcccgcctga aggagtcctt ggagatgcat atcaccccgg gcctccattc
     9721 cttctccttt cgcctgctcc cgctcttttg aaggtaaacc tgcaggttcc ggttgatctg
     9781 aaagagggta ttggcaaaga gcgattccag acctgtccgg tctttatggt aatggtttat
     9841 acatgaatag attataacta tgagcacgga agcggtcagg gcatacagtg tatttatctt
     9901 gaacatcaca atgcctgcaa taacacagcc tgtcagtgag aggtaccatt ttgacctgaa
     9961 cgacggcctg tatgaaggtg atgagccgaa atggttaagg aatgatatca ggcagagtga
    10021 accgtatgtt acgaggaaga acatggatat tatttcagcc accgcgttga cactgcccaa
    10081 cagaacaaac agagaggcga tcagcaatgt caccagtgtg gcattcaccg gttccccgtc
    10141 atgacttctt gcagatgcga gccacctgtt gagtctcata gagggaaatg aggtatcttt
    10201 tgccagagcc tgcagggttc tgggagcaac catgaccgag ccgagagcag aggtgagagt
    10261 tgaggatgcc agacccaggg gaataatgat tgctcctccg atggctatct ttcccattat
    10321 cagttggtct gcaagaagct cttccggcga ggccgagatg gccagtttcc agattacaag
    10381 gatgtaaacc accatgcctg tcagtgttgc cagtattgtg ccaagaggga tggccctggc
    10441 aggattcctc aggtctcctg acagtcccac accggcggta attccggtga aagcggggaa
    10501 gcagatggca aatacaatga agaaatcact catgttgcgt atatccctca gcggcagttg
    10561 cccggttgcc aggctgccct cagcaggttt gccaagaaag aataacagga gggtgataaa
    10621 caggatggca ttcacgacat agagcatctt cacaccggta gcggatccct tcctcagtat
    10681 catcagcccg agaatcacaa gcgccggcag gcttatcacc tgtctgggca ggataaatga
    10741 gaatcgctgt gcaacgaagt cgaacaggaa ttcgaatgac tcggcaaagg ctatgatgta
    10801 aaaggctacg ctgattgcct gtgacaggta aagtgcaatg cctattgttg cgccgatgtt
    10861 cagaccgaat gacctggata ttataaagta ttctcctcct ccttcaaccc gcttgttggt
    10921 agccagttct gagattgcca gggcggtggg gatggtcacg gcgtgtccca tgataataag
    10981 cagcagggtt ccccagaatc ccagagtccc tactgcaaag ccgaagcgga ggaacactat
    11041 ggctccgatt atggttgaaa tggccgtgaa atagacaggg gctgttccga atccggactt
    11101 cttggcagtt gcttccatca gggcaggtct gaaattagct tcatttttga gtagtaaaga
    11161 tgtaaaaaaa agagggaaaa cagtaaatta atatctttat atcagagcag aaaccaatat
    11221 gagccgggaa aaagaaacgc ttgcaggatc agatctggag gagagtgaag agcttctggg
    11281 caggctgggg attgacagac gccattcgcg aaggcttctt tactgggtct atcggcgggg
    11341 catcaggtcg tttgatgaga tcaatgacat cccgcagaag ttcctgcgca tcttgtcaga
    11401 atatgcggtt cccggcctga gttcacccac tgcttcagag gtttcttctg acggttccgt
    11461 caagtatctg ttcaccagca aaaacggcat ggcccacgag acggtatttc tgccggatga
    11521 caaacgccgg acggtatgtg tttcggtgca atcgggctgc cggatgggat gccgtttctg
    11581 tgccacagga aagagcgggt ggaacggaaa cctgactgcc ggtgagatag tcaaccaggt
    11641 tatcagcatc ccccatgaga taacccatat agtgatgatg ggcatgggtg agcctggtga
    11701 caacattgat gaggtgatac gtgcctgccg cattcttact gccgagtggg gcctggcagc
    11761 cggacgaaga cgggtaacgg tgtcaaccgt gggggtaacg ccttctgtca ggcggctgct
    11821 cgacgagact gagtgcaaca tcactctcag cctccactcg ccgtttccgg aagagaggct
    11881 tgaggtaatt cccgcagagg cgcgatggcc gtttgccgga acactcgata tgctgaaaca
    11941 gtatgataac cggattaaaa ggcgctttac cgtggcatat gtgatgattg agggaaggaa
    12001 tgattcgcag aggcaccttg aggagcttaa gagacttctt gcctgcacag gcataagggt
    12061 taaccttctt ccttatcatt ctgttgatga ggaagatgag agaagttccg gcatctctgt
    12121 catgatgcag ttcaagcatg aactggtgac atccggtatc ggagcctcag taagaaagtc
    12181 aagaggagcc gacatagcag ccgcctgtgg aatgctggct gcaaggagca ggcgcggggc
    12241 aaggaaacag taatcagatc acactgaagg atatggaagg agtagcattg gcagctctga
    12301 aagacaggag cggagcagca ttggcagccc tggcactaat actgacagcg gaataacctt
    12361 ccaggtccaa acaccggaat ctgcgcgggc agtatctatt tttccggggc gaatagtctg
    12421 aatctgcgca ggcagtatct acttatgtgg tttgataagc tggaggaggt gttcggtttc
    12481 gtaaccgtcg cttaccctgg tccactcttt cctggttccg cagatggaga agccgtgatt
    12541 ggtgaaaaga ttcaggctcg gagcattgct ctccaggatg ttgcaccaga gctggtgcag
    12601 accgatgatt tcaaaagcat attctatcac gcaggccagt gcggccgaag cgtatccctt
    12661 ctgcctgtca gactcatcgg caatgaggat gccgacaccg gcacgattgt gaaagggatc
    12721 gaattcgtac aggtcaatag tgcctattgt ccgttcatcg ggcagaacgt ctatcataag
    12781 cctgagctgg cctgtttcaa atatgctttt tcctgattcg gcaatatact ttttcaggat
    12841 ataccttgag tagggtacaa gggtatttcc ctgtctcagg ttggtttcgt cgttttccca
    12901 gcggtataac agttccaggt cttcgggctc aactgcgcga agtctgaccc cgtcattttc
    12961 cagaatcatt cctgtaaact ttctttataa agatatcacc agggtatccg aggctgagca
    13021 gcctgtccct ttcctcgtat gcacttgcct ttgagtcaaa cctgcctgtg gtgtagcggt
    13081 aaaactggtc ttcgctgtta tagaacaccg atatcttagc atactcaaaa taggctggat
    13141 caacaggatt atacaacgcc attacctgca ccgtgtaata gagtacatca ggctcaactg
    13201 tctcatcagt atcttttaca tccctgataa ccatgtcagt gatgagcata gatgagtcaa
    13261 caaccgtcag atatggcaga tcatccagat taaagctgat ggtgacagtg tcttttttct
    13321 cctcgtcagg aacattgaaa tcaacgtgag ctatctcctt cttcttgtat ccggtcatga
    13381 ttgagtagaa accctgcctg ccgttatcga acggctgaaa ggcgatgttg tcatcggttg
    13441 agctgacggg gtatcctatg ttgaccggag tggtccactg gttgtttgac agttttgcaa
    13501 ggaagatatc ataaccaccc atcgaaccat gtccttctga actgaatgtc agtgtgttgc
    13561 catctcctga gatgaagggt gtctcttcgt tgtagggagt attcaccaca ttccccaggt
    13621 tggtgggcac tccccagtct ccggtttgat ccttttccga gacccacagg tctagttcac
    13681 ccaggccgcc ttcgcggttg gaggtgaagt aaagtttctt cccgtctgat gatatggcag
    13741 cgtgcgattc gtagaatttt gtattaatgt ttttgttcag tttttcaatg tgcgaccatt
    13801 tgccgtctgt atatctggtc atatagatat tgccgtcata gttgtcattc ttgaacaggt
    13861 atagctcggt gccgtcattg ttcagtgaag ttgtgtggca gtcggttccc atggatgcct
    13921 caagggttat gtcaaccggt gttccccatg atgttccctc catgcgggtg acatatacca
    13981 cgctttcaag tcctcttctt tcggtgtatg ccattgtgtt gccatcatag ctgaccaccg
    14041 gcctttcgtt caccaggccc tggttgatgt atcccttcag gggctggatg cgtaccctga
    14101 caggattgtt catctgttcg gaggccacgt tgcaggcctg catctgctga tttatgaact
    14161 ccttgttctg catcgatgat gtttcagtaa tcagctggct gaagaacctg taagtgttca
    14221 tggcgctgtc aagcttgttg gcaatcaggt aggcatttgc cagcgagaag taagcatcaa
    14281 ggggtgcacg tgtttccttg agtttgccgg ttttggaatt gtaactggtg tttttgacag
    14341 cttcttcaag gaacggaatg gctttctgtt tctcatcgaa aatgttgagg tagcagttac
    14401 cgatcttgta cttgatattg gaattaccag gtatgagttc attaagaatg agataaagcg
    14461 ggttggccag ctcatattca ccatagaggt agtgggattc agcctccgcc catatctcct
    14521 tcagctcact tttcttttgt ccgttaagcg gcaaaagcag gagcagcagc atcagaaatg
    14581 aaattcccct aattcccata actcagttta cctgtaaaat taaacctttt gcagcatact
    14641 gtacctcttc agctacatta tttaaatctg acgcgataga tgtcagcccc tccggaactg
    14701 tcgtcccttc cggaaaatga catatatcct cctttgccat tatctgtagg acagaaaaag
    14761 atattatcat caggggtgtt cagagggtat cctatatttt ccggagtgct ccataaaccg
    14821 ttgtactgaa gctcactgca gaatatatca tagcctc
//
LOCUS       DAOPBK010000051        14797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4090_length_14797_cov_4.452856, whole genome shotgun sequence.
ACCESSION   DAOPBK010000051 DAOPBK010000000
VERSION     DAOPBK010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14797
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4090_length_14797_cov_4.452856"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLA17_04480"
     CDS             complement(<1..306)
                     /locus_tag="PLA17_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPK84703.1"
                     /translation="MDTLLRSGIKEAVRTLYSVVADDDLLQIQKTRKEFEGDFTLVVF
                     PLSKISRRSPEQTAREIGDYLKEKINAVSGYNVIRGFLNLTISPAEWSRRLAEMHADT
                     "
     gene            517..975
                     /locus_tag="PLA17_04485"
     CDS             517..975
                     /locus_tag="PLA17_04485"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HPK84704.1"
                     /translation="MGKLQDIRIEATVKTPQVSFNAATGSLTFTGKSLPENASGFFEP
                     LYKWASDYAKSPAESTNLKFNVDYFNTSSVIWMGKILKVLTKIRKSDHILFVHLYFDI
                     EEYDSMGEEDVRESLSPFLDVTADATCSVGIRLYGVDEEGNILKENIVLI"
     gene            1083..2576
                     /gene="amrB"
                     /locus_tag="PLA17_04490"
     CDS             1083..2576
                     /gene="amrB"
                     /locus_tag="PLA17_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPK84705.1"
                     /translation="MNIFGQGNDRKPVVSGSFYPSGSERLRSELSGYFASFRKPDAGV
                     KVRAIIVPHAGYVYSGHTAAAGYAAIPGEAVYDNIFLIGVSHRYAFEGAAVFSSGNLI
                     TPLGTLRVNREIGERLRSTNKWFIAKDEAHGPEHSLEVQLPFIQYHFKKPPTIVPILI
                     GTRNTTVLKAVALGLQPWFNDRNLFVISSDFSHYPSYEDARRVDKLASDAIVKGDAEA
                     FIRTIRNIEASGVDNLATAMCGWPAGAVLLYMAEKDKGLKFRNVEYTNSGDARVGSKD
                     EVVGYNAIVLEKKGSAPESLTQTATEKKSSAPESFTLTAAEKKILLGTAREAITARLN
                     HRKPVPVDAGRLTPRLREPLGAFVTLTIKGELRGCIGRFTSPDPLWEVVGAMATEAAF
                     GDPRFPALTKAEYPSVSLEISVLGPMKKINSINEIKIGRHGIYIKKGFRSGTLLPQVA
                     TERGWTVEQFLGYCARDKAGIGWDGWKDKETEIFVYEAYVFGEQEQK"
     gene            2573..4999
                     /locus_tag="PLA17_04495"
     CDS             2573..4999
                     /locus_tag="PLA17_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84706.1"
                     /translation="MTYHVVPIGVTLLVIYLFSLYLSCFGFTPRQSHRRFWNWILLFT
                     FLIAGLFGLFLALRITYRWEISFAESLLHWHVETGIAMVFASFIHLSWHLGYYFRRSR
                     IRENNVVHAAVQPDAGTDATGLRPLLLLTGFVSSASQFILLREAAILGGGTEAMAGLF
                     LWLWLIIAAGGAVTGSCSVITSMRRMVWTLLAGTALAPLCFLMMNQIILTPGQTPSFL
                     QALVILAVSVTPVTFVSALIFVRISVIRNAAGIARAGNSFTAEMIGSVAAGVLTALTV
                     TIRIPNYQLYLVILAMAVAFATWFLGYRGRIKIAVAAAMAVTATLLFIFPPDAAIRSL
                     LLRGVKAEKSIDTPFGNITTGVYSGERTVYYDHRPVFFPGDVISAEENIHYALLQRQS
                     YNRVLLVSGGLRRHLPELIKYSIGELTYLEMDPGLIAAEGVSDTLCGQMRVRVVRDDP
                     MAFLRADIGTFDAIIQLIPPPSTLSVDRFYTVEYFRMVREQLSPEGLFMCTPMPWFNY
                     SPESYRRGFSPFFNALSDVFRHVTLIPGSMLYAVASDTPVSNAVVQLAGQHGIGTSYV
                     NGDYLDDNEIKTKSEQIMSNVDRNAGMNTALRPVISLFSNILSLERMGMKGGIITLLV
                     LLMIIPFAFISRGGQAMFASSAGLAGFGMILIFILQMTAGNIYILTSVILTLLMAGLA
                     AGATLGERLGLKSLKICVLILTGIFTLTGFLAPALTVSSSGPVLTFIFVMLPAAGIVT
                     GAIYRILTSHPEGRVTGIVYSADMAGSALGYLTVATLLVPLAGTANSCFILALFILLS
                     GIVASVTGKA"
     gene            5031..6149
                     /gene="amrS"
                     /locus_tag="PLA17_04500"
     CDS             5031..6149
                     /gene="amrS"
                     /locus_tag="PLA17_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPK84707.1"
                     /translation="MNDNKNKISRRSFARRGSLALAAGFFAPFPAGAMRVAPGEKLAM
                     FQESGPRGVVCLICPNECTLKDGELSDCRNRIARKSKLYTLAYGNPCAVNIDPVEKKP
                     LYHFLPGTTTFSIATAGCNLACLNCQNWTISQTTPEKTRNYNLPPADVVTQAAAGRCR
                     SIAYTYSEPVTFYEYVYETSRLAREKGIKNIMVSNGYINREPLRQLCRYIDAANIDLK
                     SFSDSTYLKLSGGRLQPVLDSLKTYRDEGVWLEITNLIVPGWTDKPNEIRQLCDWLAE
                     NGFADTPLHFSRFHPQYKLEHLPPTPAATMTKATETAKAAGIKYVYTGNLPAAGNDDT
                     ICPSCGKKVVDRSGFRVVSQSISGGKCSCGTKIPGVWS"
     gene            6183..8225
                     /gene="ligA"
                     /locus_tag="PLA17_04505"
     CDS             6183..8225
                     /gene="ligA"
                     /locus_tag="PLA17_04505"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPK84708.1"
                     /translation="MKLSDAKRRVEELRKTIDEHNHRYYVLNQPVITDFEFDLLLNEL
                     QTLEKKFPELVTPDSPTMRVGSDLTEEFGQVEHRWPMMSLGNTYSEEELVEFGERVKR
                     TLGHEPEYVCELKYDGVSISLTYENAALSAAVTRGDGMVGDDVTVNVRTIRSIPVRIN
                     APGVPSSFVIRGEIYLPLKGFDEINAARIAKGEAPFANPRNAAAGTLKLLDPGMVAAR
                     PLDCFLYFLLGDDLPAANHYDNIMAARSWGFRTPDIMERCSGMDGVLSFIRKWESERK
                     DLPYDTDGVVVKVNSLEAQRLLGSTAKSPRWAIAYKYAAEQAVTELLSVDFQVGRTGN
                     VTPVANLRPVQLAGTTVKRASLHNSDQIELLGLHYGDTVIIEKGGEIIPKIVAVDAGQ
                     RAGDAAVVTFPDKCPECSTPLVRNEGEANHYCPNYLHCPPQITRRIIHFVSRKAMDIE
                     GLGEETVELIWSNGLIHNVADLYDLKHEQLALLDRMGDKSASNILASLAASLSAPWNR
                     KLFALGIRHVGETVAKTLATAFPDIESLMEATEEELLTLPDIGPRISASVREYFSDED
                     NREIIRRLKAAGMTFGGAVQTVTSSGPLTGKTIVVSGTFEHFTREGIRAAIEAAGGKN
                     AGSVSGNTSFVVAGSDMGPAKRAKAASLGIPVITEEEFIKMIHKDIPDNFRKLKIL"
     gene            8277..8804
                     /locus_tag="PLA17_04510"
     CDS             8277..8804
                     /locus_tag="PLA17_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84709.1"
                     /translation="MMLHRRLGALRRMKQDFSLDGVRKIGILWDASYENDFQHLAALK
                     RQLSESGKSVEVIAWIPGRDVPDKLTGLSYMKFLRKADLNWAYLPVSGDANKFLETKY
                     DLLIDVNPSSLFQLSALAALSAAPMKVGPDLTDEPENAPYDLMIRAPKPFSIALFIDQ
                     AMHYLSLIGSPRTRA"
     gene            complement(9010..12330)
                     /gene="secA"
                     /locus_tag="PLA17_04515"
     CDS             complement(9010..12330)
                     /gene="secA"
                     /locus_tag="PLA17_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPK84710.1"
                     /translation="MSIINKVLSLFLGNKEERDLKEINPFVDKIRKESALISALSNDA
                     LRDRTAALKQEIQDHIAAEEKEIADLRAEAENEEDVYRKEELYNHVDKLVKEVLEKIE
                     EKLDEILPAAFAIVKETARRFKENEVLEVTAREWDRNLAATRPSITVEGDKAYWKNRW
                     MAGGNEIVWDMVHYDVQLIGGVALHKGKISEMATGEGKTLVATLPVFLNALAGKGVHI
                     VTVNDYLSKRDSEWMGPLYEFHGLSVDCIDKHEPNSNARRKAYNSDITFGTNNEFGFD
                     YLRDNMAVNPEDLVQRKHHYAIVDEVDSVLIDDARTPLIISGPTAKSDTSQFDEYKFK
                     VEKLVNAQRKQVTQLLADSKRLITQGDNEQGGLLLLRAYKGLPKNSALIKYLSEEGVR
                     TLLQKTENFYMQNNSKEMPKVTEELFFVIDEKANSIELTEKGHDLISTSVEDSSFFVL
                     PDVGSKVADIERTIIDENERRKAKDELLQEFAVKSERVHTMTQLMKAYTLFEKDVEYV
                     VMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKENVKIEASTQTYATITLQNYFRMYH
                     KLAGMTGTAITEAGEFWNIYKLDVVVIPTNVPVIRNDHEDLVYKTKREKYNAVIDEIL
                     KLHKAGRPVLVGTTSVEISELLSRMLKMKGLKHNVLNAKLHQREADIVAEAGKSGTIT
                     IATNMAGRGTDIKLTPEVKRAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSQFFV
                     SLEDDLMRLFGSERISGIMDKLGLKDGEMIQHPMITKSIERAQKKVEENNFGIRKRLL
                     EYDDVMNSQREVIYSKRRHALLGERLSVDIANMMYDVTENVVNTYKDVSDFEEFQFAL
                     IRTLSIDSPIAKAEFTKNSNEEVADTVYEKVLEAYRRKSEIISATAYPVLKDVYERMS
                     ATYENVVVPISDGVRTYNVITNLKAAVESQGRELLRSFEKTSVLATIDDAWREHLREM
                     DDLKQSVQNATYEQKDPLLIYKFESFELFKTMINEVNQDVVSLLMKGTIPAAASTDNV
                     REAHRRRQTDMSRMRTQKSDYDSYSSSGGGSPEQRQKQMAPVRVEKKVGRNEPCPCGS
                     GKKYKHCHGQGAANSAGL"
     gene            complement(12514..13002)
                     /locus_tag="PLA17_04520"
     CDS             complement(12514..13002)
                     /locus_tag="PLA17_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPK84711.1"
                     /translation="MLTKKIEDLCNRQIEREGYSSNLYLAMASWAETKGMSGIAEWLY
                     AQAEEERAHMLKFFRYVNERNGTAIVPEFKKPPVKYKGIPELFDEVLKHEKFISASIN
                     EVLEAAIADKDYATVNWIQWFVDEQVEEESSAQMIIDKIKLAGEHGIYFLDRDIMNFR
                     GE"
     gene            complement(13059..13424)
                     /locus_tag="PLA17_04525"
     CDS             complement(13059..13424)
                     /locus_tag="PLA17_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK84712.1"
                     /translation="MAGVMIVEDDSALRELLRDALEKRKYTVIAAADGKEALAKFRPS
                     IVDLVITDLLMPEEDGLMVIMKIREIKPSTKVIAISGGGMAGPGSYLMMASKLGADAV
                     ISKPFLPSELVMKVKELLE"
     gene            complement(13455..14237)
                     /locus_tag="PLA17_04530"
     CDS             complement(13455..14237)
                     /locus_tag="PLA17_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK84713.1"
                     /translation="MSARKDKNQADNSQITPVNDPVADSRLGALAGGIAHDLNTVITT
                     IYGYSEMALEGLADSPEAAENVRRIIGAADRARMLTRQLLDLSRQAAREKVPVRVAAV
                     LDDTIDFIRPSVPEGIRILRRVTAHDAYVNAVPDQLFRVFLNLTMNAVQAMTPKGGTL
                     TVTIGCREDETGIHQGDQCRELHIRFSDTGKGMDKALQQEVFKPFVSAGKEHGTGLGL
                     SVVADAIRELGGTITVSSEPGVGSAFDIIIPGAFFGTLPEKH"
     gene            complement(14255..>14797)
                     /locus_tag="PLA17_04535"
     CDS             complement(14255..>14797)
                     /locus_tag="PLA17_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84714.1"
                     /translation="ARASQEASLSPAPATQRPKPPVTGDEPKKVSIKSIIEDAKSRDA
                     IVSEPQPDTILTFPDNEFTTEEFHEVWASFAEEIREESPRISVTLSSVTPELLADKTI
                     LLKLDNLTLKESFDDNFRQRLEGHLRRALRNGSIRLQTIVEATDRGEILYSPEQKFNH
                     LAEKNPALRELKRTFNLDFE"
BASE COUNT         3386 a         3791 c         3782 g         3838 t
ORIGIN      
        1 ggtgtcagcg tgcatttcag caagtcgtct gctccattct gccggtgata tggtcaggtt
       61 caggaatccc ctgatcacat tgtagccact aacggcattg atcttctcct tgaggtaatc
      121 gcctatctcc ctggcggttt gttcgggtga ccggcgggaa attttagaaa gcgggaagac
      181 cacaagggta aagtcgccct caaattcctt cctggtcttc tgtatctgca ggagatcatc
      241 atcagccaca acactgtaaa gtgttctgac agcctctttt atcccggatc tgagcaacgt
      301 atccatcctc tttcaattat ggctgcaaag atagcataat agttgttttc agcatccggt
      361 aattgatcct cagacaggat gatgatgaat tgttcaaaaa acttgataac tgttaattat
      421 tttttgacga atactccggc atcctcaaaa aaacaggtta cctttagctt caacaatcgg
      481 gcaattgtgc cctaacaggg aaggaatcat aatcttatgg gaaaactgca ggatattcga
      541 attgaggcta ccgttaaaac accgcaggta agttttaacg cagccaccgg aagtctgaca
      601 ttcacaggca aatcattgcc ggaaaacgct tcaggtttct ttgaaccgtt atacaaatgg
      661 gcgtcagact atgccaaaag tccggctgag agcacgaatc tgaaattcaa cgttgattac
      721 ttcaatacct cgtctgtcat atggatgggt aagattctga aagtactgac gaaaatcagg
      781 aagagtgatc atatactgtt cgtgcatctc tattttgaca tcgaagaata tgattcaatg
      841 ggtgaagagg atgtaaggga atctctctcc ccgttcctgg atgtcaccgc tgatgccacc
      901 tgcagtgttg gcatcaggct ctatggagtt gatgaagagg gtaacatact gaaagagaat
      961 atcgttctca tctgacaggg tcaacctggg ttttatcggt ttttcgttta actttacggg
     1021 atataactgc ttttgctatg aaaagggtta gtagtatcct cgtaatttta atttttccgc
     1081 taatgaatat attcggacag ggaaatgaca ggaagccggt tgtgtcagga agtttctatc
     1141 catccggcag cgagaggctc aggagtgaac tttcaggata tttcgcatca ttcaggaagc
     1201 ctgatgcagg agtgaaggtg agggccataa ttgtacccca tgccggctat gtatattcag
     1261 ggcatacggc agcggcaggt tatgccgcca taccgggtga ggctgtatat gacaacatct
     1321 tccttatcgg tgtcagtcac cgctatgcct ttgagggtgc agcggtattc agttccggga
     1381 accttatcac tccgctggga acgctgagag tcaacaggga gattggtgaa aggctcaggt
     1441 caacaaacaa gtggttcatc gcaaaggatg aggctcacgg tccggagcat tcgctcgagg
     1501 tgcaactgcc attcatacag tatcatttca agaagcctcc gactatcgtt ccgatactga
     1561 taggcaccag gaacaccaca gttctgaagg cggtagccct gggactgcag ccgtggttca
     1621 atgaccgcaa cctgtttgta atcagcagtg atttctcgca ttatccctcc tatgaagatg
     1681 ccaggcgtgt cgacaagctt gccagtgatg ccatagtcaa gggtgatgcc gaggctttta
     1741 tccgcaccat aaggaatata gaggcttcgg gtgttgacaa cctggccacg gccatgtgtg
     1801 gatggccagc aggagcggta ctgctctata tggctgagaa ggacaaggga ctgaagttcc
     1861 gaaacgttga atacaccaac tcaggtgatg ccagggtggg ctcgaaggat gaggttgtgg
     1921 gttacaacgc aatagtactg gagaagaagg gttctgcacc tgaatccctc acacagacgg
     1981 caacagagaa gaagagttcc gcacctgaat cattcacact gacggcagca gagaaaaaga
     2041 tcctgcttgg cacagcgagg gaagccatta cagcgaggct gaaccatcgc aaacctgttc
     2101 cggtggatgc aggcaggctg acgcccaggc tcagggagcc gctgggagca ttcgttacac
     2161 tgaccataaa gggtgagctc aggggatgca tcggacggtt cacctcacct gatcccctgt
     2221 gggaggtggt aggtgccatg gccacggagg cagcctttgg tgatcccagg ttcccggcgc
     2281 tgacgaaagc cgaataccct tccgttagcc tggagatttc ggttctcgga ccaatgaaaa
     2341 aaataaacag tatcaacgaa ataaagatcg gccggcacgg catctatatc aaaaaaggct
     2401 tccggtcagg aaccctgttg cctcaggtgg ccactgagcg gggctggacc gtggagcagt
     2461 tcctcgggta ctgcgcccgt gacaaggctg gcataggctg ggacggctgg aaagataaag
     2521 agacggagat atttgtctat gaagcctatg tcttcggaga acaggagcag aaatgaccta
     2581 tcatgttgtg cctatagggg tgaccctcct ggtcatctac cttttttcac tctacctctc
     2641 atgttttggg ttcaccccca ggcagtcgca tcgtcgtttc tggaactgga tactgctctt
     2701 cacctttctc attgccggcc ttttcggtct cttcctggcg ctgcgcatca cctaccggtg
     2761 ggagatctct tttgcggagt cgctgttgca ctggcatgta gagacaggca tcgcaatggt
     2821 atttgcatct ttcattcatc tctcatggca cctgggctat tatttccgca ggtcgcgcat
     2881 acgcgaaaat aacgtggttc atgccgcagt gcagccggat gcggggactg atgctacagg
     2941 actcaggccg ctgctgctgc tgacgggctt tgtaagctcg gcatcgcagt tcatactttt
     3001 acgggaggct gccatacttg gtggcggcac ggaagcgatg gcgggcctct ttctctggct
     3061 gtggctcatc attgcagccg gcggcgccgt gaccggaagc tgttcggtca ttacctccat
     3121 gcggaggatg gtatggacgc tgcttgccgg cactgccctt gccccgctct gtttcctgat
     3181 gatgaaccag ataatcctca cccccggcca gacaccgtcc tttctgcagg cactggtgat
     3241 tctggctgtc agcgtgacgc ccgtgacgtt tgtctcggcg ctcatattcg tgcgcatatc
     3301 ggtgatcagg aatgctgcag gtattgcccg ggcaggaaac agtttcaccg ccgagatgat
     3361 cggatcagta gcggcaggtg tgctgacggc gctgacggtg accatcagga tacccaacta
     3421 tcagctctac ctggtcatcc tggcgatggc agtggcattt gcaacatggt tcctcggtta
     3481 ccggggcagg ataaagatag cagtagctgc tgcaatggcc gtcacagcca ccctgctttt
     3541 catcttcccg cctgatgcgg cgataagaag ccttctcttg cgaggcgtca aagccgaaaa
     3601 gagcatcgac acacccttcg ggaacatcac tacgggtgtc tacagcggcg aacgcaccgt
     3661 ctattatgac cacaggccgg tctttttccc cggcgacgtt atttcagccg aggagaacat
     3721 tcattatgcc ctgctgcagc gccagagcta taacagggtg ttgcttgtct cgggcggact
     3781 gaggaggcac ctgcctgagc tgataaagta cagtatcggt gaactgacct acctggagat
     3841 ggaccccggc ctcattgcgg ctgaaggggt gagtgacaca ctctgcggcc agatgagggt
     3901 aagggtggtc cgtgatgacc cgatggcctt cctgagggct gatatcggaa cctttgatgc
     3961 aataatccag ttgatacctc ccccttcaac gctgtcagtt gaccgtttct acaccgtgga
     4021 atacttccgg atggtgaggg agcaactttc gcctgaaggt ctcttcatgt gtaccccgat
     4081 gccatggttc aactattcac cggagagtta cagaaggggg ttttcaccct tcttcaacgc
     4141 actctctgac gtattcaggc atgtaacgct catcccgggc tcgatgctct acgccgtggc
     4201 ctccgacacc ccggtcagca atgcagtggt gcagcttgcc gggcagcacg ggatcggaac
     4261 cagctatgtt aacggcgact acctggatga caatgagata aaaacgaaga gcgaacagat
     4321 aatgtcaaat gtcgacagga atgcagggat gaatacagct ctgcggcctg taatatccct
     4381 cttctcgaac atcctttcac tggagcggat gggaatgaag ggaggcatta tcaccctgct
     4441 ggtgctgctg atgataatcc cctttgcctt tatttcaagg ggcggacagg ccatgttcgc
     4501 ttcctcagcc ggactggcag gattcggtat gatattgatc ttcattctcc agatgaccgc
     4561 agggaacatc tacattctga catctgtcat tctgacgctt ctgatggccg gactggcagc
     4621 gggcgctaca cttggtgaac ggctggggct taagagcctg aagatatgtg ttcttatcct
     4681 gacagggata ttcaccctga cgggatttct ggcgcctgct cttacagtat catcctccgg
     4741 cccggtgctg acattcatct tcgttatgct gcctgcggca ggaattgtca caggtgcaat
     4801 ttacaggata ctgacatcac acccggaagg acgggtcaca ggcattgtct actcagctga
     4861 catggcaggc tctgccctcg gttacctcac tgttgcaacc ctgcttgtcc cgctggcggg
     4921 aacggcaaac agctgtttta tccttgcact cttcattttg ctttcaggca tagttgcatc
     4981 tgtgacggga aaagcgtaga tttacatttg gcgacagaat ccataacaga ttgaacgaca
     5041 acaaaaataa gatttccagg aggagttttg caaggagggg ttctcttgcc cttgctgcag
     5101 gcttttttgc cccctttcct gcgggagcca tgagagtggc gccgggagag aaactggcca
     5161 tgttccagga gagtggcccc aggggtgtgg tttgcctgat atgtcccaac gagtgcaccc
     5221 tcaaggatgg tgaactgagc gattgccgca accgtattgc caggaaatca aagctttata
     5281 cactggctta tggcaacccg tgcgctgtaa acatagaccc ggtggaaaag aaacctctgt
     5341 accatttcct cccgggcacc accacttttt cgatagccac tgccggatgc aacctggcat
     5401 gcctcaactg ccagaactgg accatctcgc agacaacacc ggaaaagaca aggaattata
     5461 acctgccgcc cgctgatgtg gtgacacagg ctgccgccgg acgatgcagg tctattgcct
     5521 atacctattc cgagcctgta accttctatg aatacgtcta cgaaacatcc aggctggcaa
     5581 gggagaaggg aataaagaac ataatggtat caaacggtta tatcaacagg gagccgctca
     5641 gacagctctg ccgctatatt gacgctgcca atatagatct caaatcattc agtgacagca
     5701 catatctgaa actctcaggt ggcaggctgc aacccgttct tgacagcctc aaaacatacc
     5761 gtgacgaggg tgtctggctg gagatcacca acctgattgt tccggggtgg actgacaaac
     5821 ccaatgagat caggcagttg tgcgactggc tggctgagaa cggattcgct gacactccac
     5881 tccatttcag ccgtttccac cctcagtata agctggagca ccttcctccc actcctgccg
     5941 caacaatgac aaaggcaaca gagacagcaa aggctgccgg gatcaaatat gtatataccg
     6001 ggaatcttcc cgcggcagga aatgatgaca ccatctgccc gtcgtgcgga aagaaagtgg
     6061 tggacagaag tggattcaga gtagtttcac agtccatttc cggtggaaaa tgctcatgcg
     6121 gaaccaaaat acccggagtc tggagttaaa tcatcattac aaatatcttc ccggatctca
     6181 ttatgaagct ttctgacgct aaacgaaggg ttgaagaact aaggaaaaca attgatgaac
     6241 ataaccacag atactatgtt ctcaatcaac ctgttataac tgactttgaa tttgacctgc
     6301 tgctgaatga gcttcagacg ctcgagaaaa aattcccgga acttgtaact cctgattctc
     6361 caaccatgag agtgggaagt gatctcacgg aggagtttgg acaggtggag caccggtggc
     6421 ctatgatgtc actggggaac acctacagtg aagaggagct tgttgagttt ggggagaggg
     6481 tgaagaggac cctgggccat gagccggaat atgtatgtga actcaagtat gacggggtat
     6541 ctatcagcct gacctatgag aatgcggcac tgtcagctgc tgtaacgcgc ggtgacggga
     6601 tggttggtga tgatgtgacc gtcaatgtaa ggaccattcg cagcatacct gtcaggataa
     6661 atgcccccgg tgtcccctcc tcttttgtaa tccggggcga gatatacctt ccgctgaaag
     6721 gtttcgatga gattaatgct gcccgtatcg cgaagggaga ggcgcctttc gccaatccga
     6781 ggaatgctgc cgcaggcacg ctcaagctgc ttgaccccgg gatggtggcc gcacgtccgc
     6841 ttgactgctt cctctacttc ctgctgggag atgacctccc tgccgccaat cattatgaca
     6901 atataatggc agccaggtcg tggggtttcc gtactcctga catcatggaa cgctgcagcg
     6961 gaatggatgg ggtgctgagc tttatcagaa agtgggagag tgaacgaaaa gatctccctt
     7021 atgacactga cggtgtggta gtaaaggtca actcacttga agcacagcgg cttctgggga
     7081 gcactgccaa atcgccccgc tgggccatag catacaaata tgccgcggag caggctgtca
     7141 cggagctgct atcagttgat ttccaggtgg ggagaacagg gaatgtcacc cccgttgcca
     7201 atcttcgtcc cgtgcagctt gcggggacaa ccgtaaagcg cgcatcgctg cataacagtg
     7261 accagataga actcctgggg cttcactatg gtgacactgt tataattgaa aagggcggcg
     7321 agatcatccc caagattgtt gctgttgatg ccgggcagcg tgccggtgat gcggccgttg
     7381 taacattccc cgataaatgt cctgaatgct ccacacctct ggtacgcaac gagggtgaag
     7441 ccaaccacta ctgccccaac tacctgcact gtccgccaca gatcacccgg cgtatcatcc
     7501 atttcgtctc acgcaaggcg atggatatag agggccttgg cgaagagaca gtggaactga
     7561 tatggtcaaa tggcctcatt cacaatgtgg ctgacctgta tgatcttaaa catgaacagc
     7621 ttgcgttact tgacaggatg ggtgacaaat ccgcttccaa catccttgcg tcacttgccg
     7681 cctcactctc cgcaccgtgg aacaggaaac tctttgccct gggtataagg cacgttggcg
     7741 agactgtggc gaaaacactg gccacggcat tccctgatat agagagcctt atggaggcta
     7801 ctgaagagga gttgctgacg cttcccgata taggtccgcg tatcagtgcc agcgtgcgtg
     7861 agtacttttc agatgaagac aacagagaga taataaggcg gctgaaggcg gcaggaatga
     7921 ccttcggggg agccgttcag acagttacat cctccggacc gcttaccggt aaaacgatag
     7981 tggtgagcgg taccttcgag cactttaccc gtgagggtat cagggcagcc attgaagccg
     8041 cgggaggcaa aaatgcgggg tcagtctcgg gaaacacctc cttcgtggtg gcaggaagcg
     8101 atatgggacc ggcaaaaagg gcaaaagccg cttcgctggg cattcctgta atcacagagg
     8161 aggagtttat caaaatgata cataaagata tacctgataa ttttcgtaag ctaaaaattt
     8221 tgtaggtttg cactcttatg ggattctttg tcaatttaag gttaaaagcc ggcaggatga
     8281 tgcttcacag gcgcctggga gcgctgagaa ggatgaagca ggatttcagc cttgacgggg
     8341 tcaggaagat aggaatattg tgggatgcct cctatgaaaa tgatttccag catctggcgg
     8401 ctctcaaaag gcagctttca gagagcggga agagtgttga ggtgatcgca tggattcctg
     8461 gcagagatgt tcccgacaag cttaccggtc tgtcatacat gaaattcctg aggaaagccg
     8521 atctcaactg ggcttacctc ccggtttccg gtgacgcaaa taagtttctt gaaacgaaat
     8581 atgacctgct catagatgtc aatccctctt ctctctttca gctcagtgca cttgccgcac
     8641 tttcagcagc gccgatgaag gtgggtccgg atctgactga tgagccggag aatgcaccct
     8701 atgatcttat gatcagggcg ccgaagcctt tcagcattgc attgttcatt gatcaggcaa
     8761 tgcattacct gtcacttatc gggagcccca ggacacgggc ataatcagca gcaccagccg
     8821 aagaagcccg gctctgattg ctggcgtaat ctgtatctac gaggcagccc ggctccggct
     8881 gccatggcaa ttaatatctt cgggtgagga aactaagctc tgattgctgg cgtaatctgt
     8941 atctacggac ggggcagcca agctccggcc accagcataa tcatcatctg cgggtgaagg
     9001 attacagctc tacaggcctg ctgaattagc agcaccctga ccgtgacagt gtttgtattt
     9061 tttcccgctt ccgcacgggc acggttcatt cctgcctact ttcttttcca ctctcacagg
     9121 agccatctgc ttctgtctct gttcgggcga gccgcccccg ctgctgctgt agctgtcata
     9181 atcgctcttc tgggttctca ttctgctcat gtctgtctgt ctgcgacggt gggcttcccg
     9241 tacgttgtcc gttgaggcgg cagcaggaat tgtgcccttc atcaggagac tgaccacatc
     9301 ctggttcacc tcgttaatca tagtcttgaa cagctcaaat gattcgaact tatatataag
     9361 cagcgggtct ttctgttcgt aggtggcatt ctgtaccgac tgtttgagat catccatctc
     9421 cctcaggtgt tcacgccatg cgtcatcgat agtggccagt acggaagtct tttcgaatga
     9481 acggagcagt tctctgccct gactctcaac ggcagccttg aggttggtga tgacattgta
     9541 ggtacggact ccgtcagaga tgggtacaac cacgttctca taggtagctg acatccgttc
     9601 atagacatcc ttaagcaccg gatatgccgt tgcggatatg atctcgctct tgcgccggta
     9661 ggcctcaagc accttttcat atacggtatc tgcaacttct tcgttgctgt ttttagtgaa
     9721 ttcagccttc gcaatcggcg agtcaatcga aagagtcctg atcagggcaa actgaaattc
     9781 ctcaaaatca gacacatcct tatatgtatt gactacattc tctgtcacgt catacatcat
     9841 gtttgcaata tcaacactga gtctttcacc gagaagtgca tgtcggcgct tgctgtagat
     9901 gacctccctc tgagagttca tcacatcatc atactccagc aacctcttcc tgatgccgaa
     9961 gttattctcc tccacctttt tctgggctct ttctattgat ttggtgatca tgggatgctg
    10021 tatcatctct ccgtccttca ggcccagttt gtccatgata cctgatatcc tttccgatcc
    10081 gaacaggcgc atcaggtcat cctcaagcga gacgaagaac tgtgatgatc cggggtcacc
    10141 ctgtcggcct gcgcggcccc tcagctgacg gtcaacgcgc cgtgactcat gcctttctgt
    10201 gccaatgatt gcaagtccgc ctgctctctt cacttccggg gttagcttga tgtcagtacc
    10261 acgacctgcc atgtttgtgg caattgttat ggtaccgctc tttcctgcct ccgcaactat
    10321 gtctgcctca cgctggtgca acttggcatt cagcacgttg tgtttcagcc ccttcatctt
    10381 gagcatgcga ctcagcaatt cggatatttc aacagaagta gtacccacca gaactggtct
    10441 gcctgccttg tgaagcttca gtatctcatc gatgaccgca ttgtatttct cgcgcttggt
    10501 tttgtagaca aggtcctcat ggtcattcct gataaccggg acgttggtag ggatgaccac
    10561 cacatcaagc ttgtatatat tccagaactc acctgcttcg gtaatggcag taccggtcat
    10621 gcctgccagc ttgtggtaca tcctgaagta attctgcagg gtgatggtgg cataggtctg
    10681 cgtggaggcc tcaatcttca cgttttcttt tgcctcgata gcctgatgga ggccgtctga
    10741 ataacggcgt ccctcaagga ttcgacccgt ctgctcgtca acaatcttaa ccttgttatc
    10801 catgaccacg tactccacat ccttctcgaa gagggtataa gccttcatca gctgggtcat
    10861 ggtatggacg cgctctgact tgaccgcaaa ctcctgcagg agttcatcct tggccttcct
    10921 tctttcattc tcgtcaatga ttgttctttc gatgtcagct accttacttc cgacatccgg
    10981 cagaacaaag aagctggagt cctcaactga tgttgaaatc aggtcatgtc ccttttcagt
    11041 cagctcgatg gagtttgcct tttcgtcgat gacgaaaaac agttcctctg taactttagg
    11101 catctccttg gagttgttct gcatgtagaa gttttctgtc ttctggagca gtgttctcac
    11161 cccttcttca ctcaggtact ttatgagagc actgttcttc gggaggccct tgtaggctct
    11221 cagaaggagc aggccgccct gttcgttgtc gccctgggtg ataagcctct tggagtcggc
    11281 aagcagttgg gtcacctgtt tacgctgtgc atttaccagc ttctccacct tgaacttgta
    11341 ctcgtcgaac tgtgaggtat cactcttggc ggtggggccg gatatgataa ggggcgtacg
    11401 ggcatcatcg atgagtacgg agtctacctc gtcaactatg gcgtagtgat gtttcctctg
    11461 aaccaggtct tcagggttta ctgccatatt gtcacggagg tagtcaaatc cgaattcgtt
    11521 gttggtcccg aaagtgatgt cagagttgta tgccttgcgt cgtgcattcg agttgggttc
    11581 atgcttgtca atgcagtcta ctgaaagccc gtggaactca tacagcggcc ccatccattc
    11641 ggagtcacgt ttcgacaggt aatcatttac cgtgactatg tggacgcctt tccctgccag
    11701 ggcgttaagg aacaccggca gtgtggccac gagggtcttg ccctcaccag tggccatctc
    11761 ggaaatctta cccttgtgca gtgccacccc gccaatgagc tggacatcat agtgtaccat
    11821 gtcccataca atctcatttc ctcctgccat ccagcggttt ttccagtatg ccttatctcc
    11881 ctctacggta attgaggggc gtgtggcagc aaggttgcgg tcccactccc tggcggtcac
    11941 ctcaagcacc tcgttctcct tgaagcggcg tgctgtctcc tttactatgg cgaatgccgc
    12001 gggaagaatt tcgtcgagct tctcttcgat cttttcgaga acctctttta ccagtttgtc
    12061 aacatggtta tagagttctt ccttccggta gacatcttct tcattttccg cttcggccct
    12121 cagatcggca atttccttct cctcagccgc gatatggtcc tgtatctcct gtttgagtgc
    12181 tgctgtcctg tcacggaggg catcattgga aagtgcagag atcagggcgc tctccttccg
    12241 tatcttatca acgaacggat tgatttcctt gaggtcacgt tcctccttgt ttccgaggaa
    12301 cagggagagg actttgttaa tgatgctcat ttaccgtcta tttaaaaaca gttgtaaaaa
    12361 taatacattt tatggttcct tgtgggagaa aggttcatca gatgagatgt tgtcagtccg
    12421 cagggctcac aagagtataa accgggggag ctgcaagcca taaaaaaacc gggggctgtc
    12481 ccggttttta ttgtctcttt tgtcagcgat ctgctattca cccctgaagt tcatgatgtc
    12541 acggtcaagg aagtaaatgc cgtgttcacc tgcaagtttg atcttgtcta tgatcatctg
    12601 ggcgctgctc tcttcctcaa cctgttcatc aacgaaccac tgtatccagt tgactgttgc
    12661 ataatccttg tcagctatgg ctgcctccag cacctcgttg atgctggcac taatgaactt
    12721 ctcatgttta agcacctcgt cgaacaattc ggggatgccc ttgtatttca ccgggggctt
    12781 cttgaattcg ggcacaatgg cagtaccgtt acgctcattt acatatctga aaaacttcag
    12841 catatgtgct ctctcctcct cagcctgtgc atagagccat tctgcaatac ctgacatccc
    12901 ttttgtctct gcccatgaag ccatggcaag atagaggttt gacgagtacc cttctctttc
    12961 gatctgcctg ttgcagagat cttcaatttt ttttgtcaac atagttttat ctgtttattg
    13021 gttcttcttt ccaagaaaca atccaggaat gataatgttt actccagaag ttcctttact
    13081 ttcattacca gttctgatgg aaggaatggt ttggatatca ctgcgtcggc tcccagtttg
    13141 ctggccatca tcagatagct tcccgggcct gccatccctc ctccactgat ggctatcact
    13201 ttggttgacg gcttgatttc acgaatcttc attatcacca tcagcccgtc ctcctccggc
    13261 atcaggaggt cagtaatcac aaggtcaact attgaggggc ggaattttgc cagtgcctcc
    13321 tttccgtcag cagcagcaat aaccgtatac ttgcgttttt cgagggcatc cctgagcagt
    13381 tcgcgcaggg cggaatcatc ctcaactatc attactcctg ccatatgatt tcagtgctgt
    13441 tccggcggcg caagttaatg tttttcaggg agagtgccaa aaaatgcacc gggtatgata
    13501 atatcaaaag cactccccac accgggttct gaactgacgg tgatggtacc tcccaactcc
    13561 ctgattgcat ctgcaacaac tgacaggcca agacctgttc catgctcctt gccggccgag
    13621 acgaaaggct tgaatacctc ctgctgcaac gctttatcca tcccttttcc ggtatcgctg
    13681 aagcggatgt gcagttccct gcactggtca ccctggtgaa tacccgtttc atcttcgcgg
    13741 cagccgatgg tgactgtcag ggtccctcct tttggtgtca tcgcctgaac agcattcatt
    13801 gtcaggttca aaaaaaccct gaagagctgg tcaggcacgg catttacata tgcgtcatgg
    13861 gcagtcaccc ggcgcaatat gcgtattccc tccggaactg atggcctgat aaaatcaatg
    13921 gtatcatcaa gcaccgcagc caccctgacc ggcaccttct cacgggctgc ctgacgactc
    13981 aggtcaagaa gttgacgtgt aagcatcctg gccctgtcgg ctgctcctat gattctccgg
    14041 acgttttcgg cagcctcagg ggagtcggca aggccctcaa gcgccatctc actgtatccg
    14101 tatatagtgg taattactgt attgaggtca tgtgcgatcc cgccggcaag cgctcccagt
    14161 cgcgagtcgg ctaccgggtc attcacgggg gttatctgac tgttgtcagc ctggttttta
    14221 tcttttctgg cactcacgct cttctgttaa gtctctattc aaaatcgagg ttgaatgttc
    14281 tcttcagttc cctgagagcc ggattcttct ctgccaggtg attgaacttc tgttcaggtg
    14341 agtagagtat ttctcccctg tcggtggctt ccacgatggt ctgaagcctg atggagccgt
    14401 tccggagtgc ccgccgcagg tgaccctcaa gtcgctgtct gaaattatca tcaaaactct
    14461 ccttgagggt caggttgtca agcttcagca gtatagtctt gtcggcaagc agttcaggtg
    14521 tcactgaaga gagtgtcaca gatatccttg ggctctcctc ccttatttct tcggcgaagc
    14581 tggcccatac ctcatgaaac tcctccgtgg tgaactcgtt gtcagggaag gtcaatatgg
    14641 tatctggctg tggttcactg acaatggcat cccgggactt tgcatcttca atgatgcttt
    14701 ttatggaaac ctttttgggt tcatcaccgg tcacaggggg tttgggtctt tgtgttgccg
    14761 gtgccggtga aagggatgct tcctgtgatg ccctggc
//
LOCUS       DAOPBK010000052        14796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4091_length_14796_cov_4.759107, whole genome shotgun sequence.
ACCESSION   DAOPBK010000052 DAOPBK010000000
VERSION     DAOPBK010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14796
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_14796_cov_4.759107"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..409
                     /gene="rpsO"
                     /locus_tag="PLA17_04540"
     CDS             140..409
                     /gene="rpsO"
                     /locus_tag="PLA17_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPK84715.1"
                     /translation="MYLTTEKKQEFFRTFGSSELDTGSAEGQIALFSYRISHLTEHLK
                     KNKKDFSTQRALTNLVGKRRRLLDYLKDKDISRYREIIKALNLRK"
     gene            500..2758
                     /gene="pnp"
                     /locus_tag="PLA17_04545"
     CDS             500..2758
                     /gene="pnp"
                     /locus_tag="PLA17_04545"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPK84716.1"
                     /translation="MYKLIEKSIDLGDGRSIVIETGKLAKQADGAVVVRMGKTMLLAT
                     VVAAKDAKEDTDFMPLSVEYKEKYAASGRFPGGFLKREARPGDNEILISRLVDRALRP
                     LFPDDYHAEVFVTINLISAEKDIMPDALAGLAASAALAVSDIPFHGPISEVRVSRVNG
                     EFIVNPTFDQVEIADLDIIVGATYENIMMVEGEMKEVSEDVMLEALRIAHEAIKPQCV
                     AQKELSEMVGKSVKREYCHEKNDEELRKKIWDETYDKCYSLAASGSDKKTRTDGFEAI
                     KAEFLAQFPEDAPADEMLVNRYFHDVEKEAARRLVLDEHRRLDGRKLDEIRPIWCEID
                     PLPAAHGSAIFTRGETQSLTTVTLGTKLDEKMIDEVLKQGSEKFTLHYNFPPFATGEA
                     KPLRGTSRREIGHGNLAHRALKNMMPPEETNPYAIRVVSDILESNGSSSMATVCAGTL
                     ALMDAGIQIAKPVSGIAMGLITDKDGKKFAVLSDILGDEDHLGDMDFKVAGTRDGITA
                     TQMDIKVDGLSFEVLKQALEQARRGRMHILDVMEETIAEPRPDLKPHAPRIEKIFIPK
                     EMIGAVIGPGGKIVQDIQATTGATIVIEETNGQGVVDVFGENYEVIRAALDRINAICA
                     VPEVGKVYTGKVKTITQFGAFVEILPNKDGLLHISEMDWKKVGTVEELFKEGDTVEVQ
                     LIEIDQKTGKLRLSRKPLIPKPEGWVEPPRREHTERKERQGSRDGNRGDRGNRDHRPH
                     DRRRDNNRQKSD"
     gene            2980..3624
                     /locus_tag="PLA17_04550"
     CDS             2980..3624
                     /locus_tag="PLA17_04550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPK84717.1"
                     /translation="MENIKYNYIVIEGNIGAGKTTLASMIARDCNAKLILERFADNPF
                     LPKFYNDPARYAFPLELSFLADRYGQLRDELVEPDLFKSFTVADYYFMKSLIFSSKTL
                     EQDEFNLYRQIFYIIYSSIPKPDLYVYLHVPPERLLKNIAMRGREYEKSITADYLSGI
                     QESYFTFFRQHQENRYLVLDVSNIDFVACHDDYLKVKEMIFTEPVSPGINLRNF"
     gene            3747..5453
                     /locus_tag="PLA17_04555"
     CDS             3747..5453
                     /locus_tag="PLA17_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84718.1"
                     /translation="MKNFTRLFVILLAAVLMSGCSSLDKMKKNANLVKYEISPKVLET
                     HAGIVAAQVKVTYPEKYFDKNTTLKITPVLSYEGGETAFDELLFTQGEKVLANNKPVA
                     WTGGTVNWSGDVNYIPEMKVSEFLLYIDAERKGRTLSFDPIKLADGVIATSTLVEYHA
                     MPIVLKDNYQRIVPEQKVADILYNINQSNIRPSELKSADIKALKDYIALVMENERMEV
                     KGVTNSSYASPDGPLALNERLSVERGKAADRYLKREYGKIPELAELFASQTTAEDWEG
                     FKTLVQESSIADKELILRVLSMYQDPVVREQEIKNMATAYEALADQVLPKLRRSKLIV
                     DVNLIGLSDEEILAAIKDDPSVLSLEQLLYAATLTNDTKDMLKYYEMAAEKDPKCYRA
                     WNNIGWTYLQMGKADDAMVALEKAKALKNDDNVKNNMGFAALLKGDISAASEYFNSMS
                     AATPESKFGLGTIAIHEGKYDQAVNLLSDTPTMNLALAQLLKGDINKAKMTMDAVEPC
                     KCGAPSYLKAVIAARLDNKDGVINGLREAFGYNAKWKNYALTDLEFAKYFTDDAFIAV
                     TK"
     gene            complement(5514..6044)
                     /locus_tag="PLA17_04560"
     CDS             complement(5514..6044)
                     /locus_tag="PLA17_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPK84719.1"
                     /translation="MRKLTNDELNRKSFTEFRASEKSPFIIVLDNVRSLNNVGSIFRT
                     ADAFLVEAIWLCGITATPPHREIHKTALGATESVEWRYFESSADAVRQLKERGYTIVS
                     VEQAEGAVSPDLFVTEPEKRYALVFGHEIRGVTEEVVNLSDACIEIPQYGTKHSFNVA
                     VSAGIVLWELAGRIKR"
     gene            complement(6101..6247)
                     /locus_tag="PLA17_04565"
     CDS             complement(6101..6247)
                     /locus_tag="PLA17_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84720.1"
                     /translation="MNLSAALVNRGVVMTISVHDCPGLPVTSAPVNVEAAMTFSPELS
                     ISAR"
     gene            6351..8363
                     /gene="priA"
                     /locus_tag="PLA17_04570"
     CDS             6351..8363
                     /gene="priA"
                     /locus_tag="PLA17_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPK84721.1"
                     /translation="MSRQFADIIIPVAVPGSFTYEVPEHLQGAVRRGSLVNVPFGQSR
                     KSTGLVVKIHEIPPAEVSLREIDSLLPGETAVNDRLTDFLLWISDYYMAYPGEVMKAA
                     IPSADDLPGRVLKRRQKEEPDVPESELIMPAELNRVQMEAYNSVKQLFADRETVLLHG
                     VTSSGKTEIYIHLMREQLDQGKQVLYMLPEIALTTQIIERLRKHFSSAIGVFHSRLTP
                     AARRKVWKKVSEGSLRAVLGVRSSLFLPFSDLGLIIVDEEHDSSYKQYDPAPRYHARD
                     AGMMLARIHGGRVLLGSATPSLESYHNALSGRYGFVELLTRYGEVMMPEMVIADTRRG
                     GKKRGPSARFSPLLLEAMEEALKREEQVILFQNRRGFSPFLMCSDCGHVPACTNCSVS
                     YTYHKSINRLVCHYCGKSVRPPSECPDCGSVNISTMGFGTERIEEEIRLLFPSARVAR
                     MDQDTVRNRGAASDILSSFAAGETDILIGTQMISKGLDFERLTVVGILDADNMMHFPD
                     FRAHERSFQLMEQVSGRAGRRTRRGKVIIQTADPANMVLRQVLKHDYRSMSSAQLEER
                     ALFGYPPFTRIIRIALKHRDLDELSASAARLADTLRRHLGNRVLGPEFPMVMQVQKWY
                     IKTVMIKIDSTLSPSKVKELVRRTMESELKYPRKGVLRIHADVDPQ"
     gene            8420..8791
                     /locus_tag="PLA17_04575"
     CDS             8420..8791
                     /locus_tag="PLA17_04575"
                     /inference="COORDINATES: protein motif:HMM:NF039364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HPK84722.1"
                     /translation="MKIEVSNGEIADKLTIIELKLERISDPDKLKNLKKEHAILDEAV
                     MSFMKKDDPLYRELYQINSELWEIEDRIRDLERNKDFGEEFIQTARSVYFSNDKRSEI
                     KRKINMITGSGLVEEKSYEKY"
     gene            8793..9818
                     /locus_tag="PLA17_04580"
     CDS             8793..9818
                     /locus_tag="PLA17_04580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPK84723.1"
                     /translation="MSLLVIRTSSMGDVIMTLPVLRSFAAAYPGEELVFVTRKPFDQF
                     VTGIPGVKIFLFDHRGRHRGFRGILRLWNDLRSMYHITAVADLHDVIRSKVVRLLFRM
                     GGCHVARIDKERIRRRKLVRGRLTGSLTHTVERYRTVFKQAGYPLKLISGPWLLPSDE
                     GRAVAAARTGGMKRPFLGVAPLAKHPLKVWPLEKMEAMLRMLMSRKECTIFLFGSPDE
                     SAVVDEIAARVPGAISMAGKISLGGEIALIADLDLMVAMDSSNMHLAAMTGVETLSIW
                     GGTHPRAGFSAWGMPPENQIQIPDTELTCRPCTIYGKGTCRRGDLACLEWLTPEAVLQ
                     KILNKLN"
     gene            9822..12149
                     /locus_tag="PLA17_04585"
     CDS             9822..12149
                     /locus_tag="PLA17_04585"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPK84724.1"
                     /translation="MKFTGTNHLIIALAFIALAGAGCRVTPEGSADRISAEPADLVNT
                     LMGTDSEYLLSHGNTYPAVALPFGMNFWTPQTRDNGNGWGYTYDDHRIVGIKQTHQPS
                     PWINDYAAFSLMAVTGGNRLTEKARASWFSHKAEVAKPWYYSVYLADYDITAEVTPTE
                     RAALFRFTFPESDSSAVVIDAFAGGSMVRIIPESNRVVGYCRNNHGGVPENFHNYFVI
                     EFDRQFDFWSLVLDGTLRPGVMETGGDHAQAVIGFRTERGLQVTARVASSFISPEQAI
                     LNLERETGGRGFDAVRDAGRERWNAELGRIKVEGGTDDQKRTFYSTLYRTMLFPRMFH
                     EIDNSGNIVHYSPYNGKVLPGRLFTDNGFWDTFRAAMPLITIIYPDLSSHIMEGLVNT
                     YLESGWLPEWASPGHRNCMIGSNSASLIADAYLKGIRGYDIDTLWKALVKNTLGHGPV
                     HSVGRYGAEYYNSLGYVPYDVGINENVARTLEYAYADWCLWKLAQELGRPEEETALYE
                     ARARNYKNVFDSERKLMRGKNLDGTFQSPFSPYKWGDAFTEGNSWHYTWSVFQDVAGL
                     AELMGGDREMARMLDSVFVVPPLFDDSYYGFPIHEIREMQIMNMGNYAHGNQPVQHMI
                     YLYNYTPEPWKAQMRVREVMDKLYNYTPDGYCGDEDNGQTSAWYVFSTLGFYPVTPGT
                     GEYVLGSPLFTRATVTLSDGRKLVINAPKNSRDNIFVRDVTLNGKKLNLNFVTHEELT
                     AGGTLEFDMSNEPAMERGTTPSARPYSMSTDDRQN"
     assembly_gap    12264..12363
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12440..12889
                     /locus_tag="PLA17_04590"
     CDS             12440..12889
                     /locus_tag="PLA17_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPK84725.1"
                     /translation="MSLTERIAKDLMAAMKAQDKGVLEALRAAKTAFILARSERGQDT
                     VLTEEEEIKIIRKLVKQRKESAAIYREQKRPDLCEKEENEASVLERYLPAAMTEQQLE
                     AVLREIISRIGAKGPSDMGRVMGVATKELAGKADGREISAKVKQLLG"
     gene            complement(12904..13485)
                     /locus_tag="PLA17_04595"
     CDS             complement(12904..13485)
                     /locus_tag="PLA17_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84726.1"
                     /translation="MSALETLKNLVLAPYILKATALISVQRAVGGNQFRHCFSTLGIL
                     LDYKFFTDTVLLKASLLHDLLEDLPETQVEEIRCIDSDGTNVVQLVLEVTKRKDETKE
                     QYLQRLLEYGSRDALLLKCADRISNITDLHRDIQSEQKISDYLDQTEKYVIPMAKRVN
                     NDMFIELTDLVAKRRGILRMLENNKRAETVPGL"
     gene            13796..>14796
                     /locus_tag="PLA17_04600"
     CDS             13796..>14796
                     /locus_tag="PLA17_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPK84727.1"
                     /translation="MILTRVLPAFVLIITMVAPGVVIAQVPVEVSTEKVVSGGRVYYM
                     HEVQKNQTLYSIAKAYRVSVDDLSRENVIPPNGIQAGQMLKVPAPDQEGSATSQSRPP
                     AQQAAAVTQPRPAVQPSRPAIASGVPGIDISGEKIVSGGKTYYMHEVQKGQTLYSIAR
                     AYRVTILDIDRENSIPPGGIQTGQVLKIPASLAFEVIEEQTPPADAAATPVRGTATAT
                     NAPRGNTAADAKIPGQATARQQTRMPFQEQPAARSDTEKPVVDDDMTVKTTEVKENAD
                     VKTQTAAKAQPEPEMQQQAQPEVKTEQHAQPEAETRQQAQQQTPSTGKSDDQPKPVQP
                     "
BASE COUNT         3843 a         3600 c         3874 g         3379 t
ORIGIN      
        1 acttccccgc atgctgctga ggatgcccgg cgttaaatac aaaaaaaatt tgttcaaatt
       61 gtttgaatta ttacaaaata gagttgtatc tttgcgttcc aaaattggag aaattattaa
      121 caatctattg gataaggata tgtatttgac cacggagaag aaacaggaat ttttcaggac
      181 attcggatca tcagagttgg acacaggcag tgctgagggt cagattgctc ttttctccta
      241 taggatttcg cacctcaccg agcatttgaa gaagaacaag aaggatttca gtacacagag
      301 ggcgctgacc aatctggtcg gaaaaagaag gcgtttgctc gactatctga aagacaagga
      361 tatcagcagg tatcgtgaga tcatcaaggc actgaacctc cgaaaataag caaaaaaggc
      421 aatttcatat tgcctttttt aattcccgga gagaaatgcc ccttgactat catttatccc
      481 gtaataatca gtagtttaga tgtataagtt aatcgaaaaa agcattgacc ttggcgatgg
      541 ccggtcaata gtaattgaaa cgggcaagct ggccaagcag gccgacggtg ctgtggttgt
      601 aaggatgggt aagaccatgc ttctggcaac cgtcgttgca gctaaggatg caaaggaaga
      661 cacagacttc atgccgctgt ccgtagagta caaggaaaag tatgcggcat cgggccgttt
      721 tcccggaggc ttcctcaaga gagaggcaag acccggtgac aatgagattc tgatctcacg
      781 actggttgac agggcgctgc gtccgctctt cccggacgac tatcacgcgg aagtgtttgt
      841 aaccattaac ctcatctcgg ccgagaagga tatcatgcct gacgcgcttg ccggactcgc
      901 cgcatcagcg gcactggcag tgtctgacat acctttccat ggcccgatat cagaggtaag
      961 ggtatcaagg gttaacggcg agttcatcgt caatcctaca tttgatcagg tggagattgc
     1021 ggaccttgac atcattgtcg gagcaacata tgagaatatc atgatggttg agggtgagat
     1081 gaaggaggtt tcagaggatg tcatgctcga agcgctgcgc atcgcccatg aggctatcaa
     1141 accgcagtgc gttgcacaga aggaactctc tgaaatggtc ggcaagtcag taaagagaga
     1201 gtactgtcat gagaagaatg atgaggaact ccgcaagaag atatgggatg agacttatga
     1261 caagtgttac agtctggctg ccagcggctc tgacaagaag acccgcactg acggctttga
     1321 agcaataaag gctgagttcc ttgcacagtt cccggaggat gcaccggcag atgagatgct
     1381 ggtgaacagg tacttccatg atgtggagaa ggaggctgcc cgtcgtcttg tgcttgatga
     1441 gcacaggcgt cttgacggcc gcaagcttga tgagatccgc cccatctggt gtgagattga
     1501 tccgcttcct gcagcccatg gctcagctat cttcacccgc ggtgagacac agtcgctcac
     1561 cactgtcacc cttggcacca agcttgacga gaagatgatt gacgaggtgc tcaaacaggg
     1621 ttccgagaaa ttcacgcttc actataactt tccccctttc gccaccggcg aagccaaacc
     1681 attgcgcggt accagccgca gggagatagg tcacgggaac ctggcacaca gggctctcaa
     1741 gaacatgatg ccgcccgagg agaccaatcc atacgcaata cgtgtggtat cagatatcct
     1801 cgaatcaaac ggctcatcat cgatggcaac cgtctgcgcc gggacactgg ccctcatgga
     1861 tgcaggtata caaattgcca agccggtttc cggtatcgct atggggctga ttactgacaa
     1921 ggacgggaag aaattcgcag ttctttcaga tatactgggt gatgaagacc atctcggcga
     1981 tatggatttc aaggtcgcag gcacccgcga tggcattacc gccacacaga tggacatcaa
     2041 ggttgacggt ctttcgttcg aggttctcaa acaggcgctc gaacaggcgc gccgtggcag
     2101 gatgcatatc ctcgacgtca tggaagagac cattgccgag cccaggcctg acctcaagcc
     2161 tcatgcacct agaatagaga agatttttat acccaaggag atgataggtg ctgtgatagg
     2221 ccccggcgga aagattgtac aggatattca ggccaccaca ggtgcaacta tagttatcga
     2281 ggagaccaac ggccagggtg tggtcgatgt ctttggcgag aactacgagg ttatcagggc
     2341 ggcccttgac cgtatcaacg ctatatgtgc tgttcctgag gtaggaaagg tctataccgg
     2401 caaggtgaaa accataacac agttcggtgc gtttgttgag attctcccga acaaggacgg
     2461 actgctgcac atctcggaga tggactggaa gaaggtaggc actgttgagg agctcttcaa
     2521 ggagggtgac actgttgagg tgcagcttat tgaaattgac cagaagaccg gcaagctgag
     2581 gctctcgcgc aagcccctca tcccgaagcc ggaaggctgg gttgaaccgc ccaggcgcga
     2641 acatacggaa agaaaggagc gtcagggatc acgtgacgga aaccgcggtg atcgcggaaa
     2701 ccgtgaccat cgcccgcatg accgccgtcg tgacaacaac cggcagaaat ccgattgatc
     2761 aatcacaact gacagaaatc cggtggatca ttacaaccgg caaaaatccc cggttgatca
     2821 tccaggatag ttattaaggc ggaggctgtc tcataaaagg gacagcttct cttttgtgca
     2881 ccctgttaca actatttcgc ggaaacgcgg gtcttcgatt tatttgcatt gtgaaggcta
     2941 aatgttaatt ttgatatccg gtgcaatgtg gcaacaggga tggagaacat aaagtacaac
     3001 tatattgtta ttgaggggaa tatcggtgcg ggaaagacta ccctggcgtc aatgattgca
     3061 agggactgca atgccaaact gatccttgaa cggtttgccg ataacccttt cctccccaaa
     3121 ttctacaatg atcccgcacg gtacgccttc cccctcgagc tatccttcct ggctgaccgt
     3181 tacgggcagc tcagggatga actggtggaa ccggatcttt tcaaaagctt cacagtggct
     3241 gactactact tcatgaagtc gctcatcttc tcttccaaaa ccctggaaca ggatgagttc
     3301 aacctttacc gccagatatt ctatattatc tatagctcca ttcccaagcc tgacctgtat
     3361 gtttacctgc atgtaccccc tgagagactg ctgaaaaaca ttgcaatgag gggtagggag
     3421 tacgaaaagt caattactgc cgattatctc tcgggaattc aggagagcta ctttaccttc
     3481 ttcaggcagc atcaggagaa ccgttacctg gtgctcgatg tctcaaatat tgactttgtt
     3541 gcatgccatg atgactacct taaagtgaag gaaatgatct tcaccgaacc tgtctccccg
     3601 gggattaacc tccggaattt ctgatttttt cattcaggtg ttgcaggtaa tgactaaaaa
     3661 atatcttaaa tttgcagaca aatcagaaat atcgtataat tgctctaatt aacagaaact
     3721 gatcgcaact aattaaaaca cacattatga aaaatttcac cagacttttt gtcattctgc
     3781 ttgctgctgt acttatgtca ggttgcagca gtcttgacaa gatgaagaaa aatgccaatc
     3841 tcgtcaagta tgaaatttcc cccaaagtgc ttgagactca tgcaggcatt gttgcagcac
     3901 aggttaaggt aacctatccg gagaagtact ttgacaagaa caccacgctg aagatcaccc
     3961 ctgttctttc atatgaaggc ggtgagacgg catttgatga actgctcttt acccagggag
     4021 agaaagttct ggccaataac aaaccggttg catggactgg cggaacggta aactggtcag
     4081 gtgatgtaaa ctatatacct gagatgaagg tgtctgagtt ccttctttac atagatgctg
     4141 aaagaaaggg caggactctc tcatttgatc ccatcaagct ggctgacggt gtaattgcaa
     4201 cttcaaccct tgttgaatat cacgcaatgc ccatcgtact gaaagacaac tatcagcgca
     4261 ttgttcccga gcagaaggtg gccgacattc tttacaacat aaaccagtcg aatatcaggc
     4321 cttcagaact gaagtctgct gatattaagg ctctcaagga ttatattgca cttgtaatgg
     4381 agaacgaaag gatggaggtt aagggtgtaa ccaacagctc ttatgcttca cctgatggtc
     4441 cgcttgcact taacgaaagg ctttcagttg agaggggtaa ggctgctgac aggtacctca
     4501 agagggaata tggcaagata cccgaactgg ctgaactatt cgcaagccaa accactgctg
     4561 aagactggga aggtttcaag actctcgtgc aggagtcgtc aatcgctgac aaggaactca
     4621 tcctgagggt tctctcgatg taccaggatc ctgtcgtccg cgaacaggag atcaagaaca
     4681 tggcaactgc atatgaagca cttgcagatc aggttcttcc gaagctgaga aggtcaaaac
     4741 tgatcgttga cgttaacctg atcggcctca gcgatgagga gatactcgcc gccatcaagg
     4801 atgatccgtc agtactcagc cttgagcagt tgctctatgc tgccactctg accaatgata
     4861 ccaaggatat gctgaagtat tatgagatgg ctgccgagaa ggatcccaag tgttacaggg
     4921 catggaacaa tatcggctgg acttatctgc agatgggcaa agctgatgat gccatggtag
     4981 ctcttgagaa ggccaaggct ctcaagaatg atgacaacgt gaagaacaac atgggctttg
     5041 ccgctcttct gaagggtgat atcagtgcag cttccgagta tttcaactcg atgtcagccg
     5101 ccactcccga atcaaaattc ggacttggca caattgcaat tcatgaaggc aaatatgacc
     5161 aggcagtcaa cctgctcagt gacacgccca caatgaacct tgcactggca cagcttctca
     5221 agggtgacat caacaaggcc aagatgacca tggatgcagt tgaaccctgc aaatgcggcg
     5281 ctccctcata cctcaaggct gtaatagcag cacgccttga caacaaggat ggtgtcatca
     5341 acggactgcg cgaagcattc ggttacaacg ccaaatggaa gaactacgct ctgacagacc
     5401 ttgagtttgc caagtacttc acagatgatg cattcattgc agtaacgaag taacaaaacc
     5461 acagatattg aaaaaccccg tccaccacat ggcagacggg gttttttaat atgttagcgt
     5521 ttgattcttc ctgccagctc ccacaggact atcccggcac tgacagccac attgaatgag
     5581 tgtttggtgc catactgggg tatctcgatg catgcgtctg acagattcac cacctcttcg
     5641 gtaacccctc tgatctcatg gccgaatacc agtgcatacc tcttttctgg ctccgtcaca
     5701 aacaggtcag gtgatacggc tccctcggcc tgctccacgg aaacaatggt gtaacccctc
     5761 tccttaagct gcctgacagc atcggcagag ctctcaaagt acctccattc aaccgattca
     5821 gtcgccccca gcgcagtctt gtgtatctcg cggtgagggg gtgtggccgt gatgccgcaa
     5881 agccatattg cctccaccag gaaagcgtcg gccgtcctga aaattgaccc cacgttgttg
     5941 agactgcgaa cgttgtcaag gacaataata aaaggagact tctccgaagc tctgaattca
     6001 gtaaaactct tccggttcag ttcatcgttt gtcagcttcc tcattggttg aaattttaac
     6061 aaaaatagtt ataaaaagga gtaaattacc tgtctgttct tcacctggca ctgatagaaa
     6121 gctcagggct aaaggtcatt gccgcttcaa cattcaccgg tgccgatgtt accggaaggc
     6181 cagggcagtc atgaactgat atggtcatta ccactcctct gttcaccagt gctgccgaaa
     6241 ggttcatata agccctgaac caatatggtc attgccctca agttcataaa ttcaggccgg
     6301 agagatggcc ttttttgacg tttattctta atttgccgct gtttcaacca atgagcagac
     6361 aattcgcaga tatcatcatt cctgttgccg tgcccggctc atttacctat gaagttccgg
     6421 agcatcttca gggtgcagtc cgccgcggca gcctggtgaa cgttcccttt ggtcagagca
     6481 ggaagagtac cgggctggtg gtaaagatcc atgagatacc accggcagag gtctctctca
     6541 gggagattga cagcctcctt cccggcgaaa cggctgtcaa tgaccgtctt accgactttc
     6601 ttctgtggat atctgattat tacatggcct atccgggcga ggtgatgaag gcagcaatac
     6661 cgtcggccga tgatcttccc gggcgcgtgt tgaaacgcag gcagaaggag gagcctgatg
     6721 tgccggagag tgagttaatc atgccggcag agctgaacag ggttcagatg gaggcctata
     6781 attcagtaaa acagctgttt gcagaccgtg agacggtgct gctgcatggc gttacctcca
     6841 gcggcaagac cgagatatat attcatctta tgcgtgagca gcttgaccag gggaagcagg
     6901 tcctttacat gcttcctgag atagctctta caacgcagat cattgagaga ctcaggaagc
     6961 atttcagcag cgccatcggg gtgtttcact cacgtcttac ccccgcggca cgaagaaaag
     7021 tgtggaagaa agtcagtgag ggaagcctca gggctgttct gggtgtacgc tcatcactct
     7081 tccttccatt cagtgacctg ggtctgatca tagtggatga ggagcatgac tcatcataca
     7141 agcagtatga tccggccccg cggtatcatg cacgtgatgc cggaatgatg cttgcccgca
     7201 tacatggagg cagggtgctc ctcggttcag ccacgccgtc acttgaaagt taccacaacg
     7261 cactctccgg aaggtacggg ttcgttgagc tgctgacacg ttacggtgag gtcatgatgc
     7321 ctgagatggt catcgctgac actcgccggg gtggtaaaaa gaggggtccc tcggctcgtt
     7381 tttcacccct tcttcttgag gcgatggagg aggcgctgaa gcgcgaggaa caggttatcc
     7441 tcttccagaa caggagaggc ttctcaccct ttctgatgtg ctctgactgc ggacatgtcc
     7501 ctgcctgcac caactgttcg gtaagctaca cctatcacaa aagcatcaac cggctggtgt
     7561 gccactactg cggcaagagc gtgagaccac cctcggagtg ccccgattgc ggatcagtga
     7621 atatctccac catgggcttc ggcactgaaa gaatcgaaga ggagataaga ctgctgttcc
     7681 cttcggcgcg cgttgcgcga atggatcagg acactgtcag gaacaggggc gctgcctccg
     7741 atatactgag cagcttcgcc gcaggcgaaa ccgatatcct tatcgggaca cagatgatct
     7801 ccaagggact cgacttcgaa agactgacgg tagtgggtat cctcgatgcc gacaatatga
     7861 tgcatttccc cgatttccgg gctcacgagc gcagcttcca gctcatggaa caggtgagcg
     7921 gaagagcagg aaggaggaca cgccggggga aggtgattat ccagaccgct gaccctgcaa
     7981 acatggtcct cagacaggtg ctgaaacatg attacaggtc gatgagctcg gcgcagcttg
     8041 aggagagggc tctcttcggc tatcccccct ttacaaggat aatcaggata gcgctgaaac
     8101 accgtgacct tgacgagctt tctgcctcgg cagcaagact ggctgacacc ctccgcaggc
     8161 acctcggcaa ccgtgtgctg gggccggagt ttccgatggt gatgcaggtt cagaagtggt
     8221 acatcaaaac ggtgatgata aaaatagaca gcacgctctc cccctcgaaa gtcaaggaac
     8281 tggtgaggcg cacaatggag agcgagctga aatatcccag gaaaggagtg ctgcgcattc
     8341 atgccgatgt cgatccgcaa tgatctcatt tgccctttaa tttttaaagg ttatttttac
     8401 agaatcaaag ctgtttttaa tgaaaataga ggtttcaaac ggagagatag cagacaaact
     8461 gacgattatt gaactgaagc tggaacggat cagtgacccc gataaactga agaacctgaa
     8521 aaaggagcat gccattcttg atgaggcggt tatgagtttc atgaagaaag atgacccgct
     8581 gtaccgggaa ctctaccaga tcaactccga attgtgggaa attgaagacc ggataaggga
     8641 ccttgaaaga aacaaggatt ttggagaaga attcattcag actgccaggt cagtatactt
     8701 ttcaaatgac aagcgttccg aaatcaaacg gaagattaac atgataaccg gttcgggtct
     8761 cgtagaggaa aagtcgtacg aaaaatactg agatgagcct tctcgtgata cgcacatcat
     8821 cgatgggtga tgtgattatg acactgcccg tgctcaggtc gtttgccgcc gcatacccgg
     8881 gcgaagagct ggtctttgtc accaggaagc cttttgatca gtttgttacc ggcattccag
     8941 gggtgaagat ttttcttttc gatcatcgtg gcaggcacag gggtttccgg ggcatcctga
     9001 gattatggaa tgatcttcgg tcaatgtatc acatcactgc ggtggctgac ctccatgacg
     9061 ttatcaggtc gaaggttgtg cgcctgttgt ttcgtatggg cggctgccac gtggccagga
     9121 tagacaagga gcgtatccgc cggcgcaagc tggttcgcgg gaggctcacc ggctcgctca
     9181 cccataccgt tgaacgctac cgcaccgtat ttaagcaagc cggatatccg ctgaagctga
     9241 tctccggccc ctggttatta ccctctgatg aagggagagc ggtggcagcc gcccgcaccg
     9301 ggggcatgaa aagacctttc ctgggagtgg cgccgctggc gaaacaccct ctcaaggtat
     9361 ggcccctgga gaagatggag gccatgctcc gcatgttgat gtcgcgaaag gaatgcacaa
     9421 tattcctttt tggttctcct gacgagtctg ctgtagtgga tgagatagct gcccgtgtgc
     9481 ccggagccat atccatggca gggaagatca gcctgggagg agagattgca cttattgctg
     9541 accttgacct gatggtagcc atggactcgt caaacatgca cctggctgcc atgacgggtg
     9601 ttgaaacgct ctcaatatgg ggcggcaccc atccccgtgc cggattcagt gcctggggta
     9661 tgccgcctga aaaccagata cagataccag ataccgaact cacatgccgt ccctgtacca
     9721 tctacggaaa agggacctgc cggcgtggcg acctcgcctg cctggagtgg ctgacaccgg
     9781 aggcggttct gcaaaaaatc ctgaataaac tgaattgaca tatgaaattc accggaacca
     9841 accatctgat tattgctttg gctttcatag cccttgccgg cgccgggtgc agagtgacac
     9901 ctgaaggtag tgcagaccgc atctctgcag aacctgctga cctggtaaac acacttatgg
     9961 gtacagattc cgagtacctg ctctctcacg gcaacaccta tccggcagtg gcattgccgt
    10021 tcgggatgaa cttctggaca ccgcagacac gtgataatgg caacggctgg ggatacacct
    10081 atgatgacca taggatagtg ggtattaaac agacacatca gccaagtccc tggataaatg
    10141 attatgcagc cttctcgctg atggctgtca cgggtggcaa ccgtctcacc gagaaggccc
    10201 gtgcatcatg gttctcgcat aaggctgagg tggcaaagcc atggtattac agcgtttacc
    10261 tcgccgacta tgacatcact gcagaggtga cacccacgga gagggcagcc ctcttcaggt
    10321 tcacatttcc tgagagcgac tcctccgctg tggtcattga tgcctttgcc ggcggatcaa
    10381 tggtaagaat cattccggaa agcaacaggg ttgtgggtta ctgccgcaac aaccacgggg
    10441 gagtgcctga aaacttccat aattattttg tcattgagtt tgaccggcag tttgacttct
    10501 ggtcgttagt ccttgacggc actctccggc ccggtgtcat ggagaccggt ggtgaccacg
    10561 cccaggcggt cattggattc cggactgaaa gaggcctgca ggtaactgcc agggtggctt
    10621 catcattcat ctctcccgaa caggccattc ttaatcttga acgtgagacg ggcgggcgcg
    10681 gattcgatgc tgtccgtgac gccggacgtg aacgctggaa cgctgagctg ggccgcataa
    10741 aggtggaggg cggtactgat gaccagaagc gtaccttcta ttcaaccctc taccgcacga
    10801 tgctcttccc ccggatgttt cacgagattg acaactccgg gaatattgtt cactacagtc
    10861 cttataacgg caaggtgctt ccaggcaggc tcttcaccga caatggcttc tgggatacct
    10921 tccgtgctgc gatgccgctt attaccatta tctaccccga tctgagctcg cacatcatgg
    10981 agggtctggt caatacatac ctggagagcg gctggctgcc cgagtgggcc agtcccggcc
    11041 accgcaactg catgataggg agcaattccg cctcactgat agctgatgcc tatctgaagg
    11101 gaataagggg atatgatatc gataccctgt ggaaggcatt ggtgaagaac accctcggcc
    11161 acggtccggt gcattccgtc ggcaggtacg gagctgaata ctacaacagc ctgggttatg
    11221 taccatacga tgtgggcata aatgagaatg tggcgcgtac actggagtat gcctacgcag
    11281 actggtgcct gtggaagctg gcgcaggagc ttggaaggcc ggaggaagag actgcccttt
    11341 atgaagcccg ggcccggaac tataaaaacg tttttgacag tgagagaaag ctgatgaggg
    11401 gcaaaaacct cgacggtaca ttccagtcgc cgttcagccc ctataaatgg ggtgatgcct
    11461 tcacagaagg aaacagctgg cattacacct ggagcgtctt tcaggatgta gccggactgg
    11521 cagagctgat gggtggcgac agggagatgg cacgtatgct cgattcagta tttgtggtgc
    11581 cgccgttgtt tgacgactcc tattacggct tccccattca tgagatacgt gagatgcaga
    11641 taatgaacat gggcaactat gcccacggca accagccggt tcagcatatg atctacctct
    11701 acaactacac gcctgagcca tggaaggcac agatgagggt acgcgaggtt atggacaagc
    11761 tttataacta tacacctgac ggttactgcg gagatgaaga taacgggcag acctcagcct
    11821 ggtatgtttt cagcactctg ggtttctatc cggtgacgcc cggaacaggc gaatatgtac
    11881 ttggctcacc gctgtttacc agggcaacgg taaccctctc tgatggaagg aagctggtaa
    11941 tcaatgctcc gaagaacagc cgtgataaca tatttgtaag agatgtaacc cttaatggca
    12001 aaaaactgaa tctcaatttt gtaacccatg aagagctgac cgcaggaggc accctggagt
    12061 ttgacatgag caatgaacct gccatggaga ggggtacaac gccttcagcc agaccatatt
    12121 cgatgagcac agatgacaga caaaattgat ttttcagatc gtcgcctggg ctctcctcct
    12181 gcttcttcgg ctacaggttt ccctggtttt tctccggtct gtggcaacag ttcaaaaagc
    12241 tcttctcctg cctgcggcga cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnngggtgat ttccacttgt ggcaacaggg agtagtataa attcgtatat tcatgaacat
    12421 aataatacca atcagcaaca tgtcactcac agaaagaatt gcaaaggacc tgatggcagc
    12481 catgaaggct caggacaagg gggtacttga agccctgagg gcggcaaaga ccgcgtttat
    12541 tcttgccagg tcagagagag ggcaggatac cgttctcact gaagaggagg agataaagat
    12601 catccgtaaa ctggtgaagc agaggaagga gtcggcagcc atataccgcg agcagaagag
    12661 gcctgacctt tgtgagaagg aggagaatga agcctcagtg ctggaaagat atcttcctgc
    12721 agcaatgaca gagcagcagt tggaagctgt tctcagggag atcatcagcc gtataggggc
    12781 aaagggcccg tcagacatgg ggcgtgttat gggtgttgcc acgaaggaac tggcaggcaa
    12841 ggccgatggc cgtgagattt cagcaaaggt gaagcaactg ctgggataag gcggcagggc
    12901 cggtcataaa ccgggtactg tttccgccct cttgttgttc tcaagcatac gcaggatacc
    12961 gcgacgcttt gcaaccaggt cagtcagctc gataaacatg tcattgttga cccttttggc
    13021 cattggaatg acatattttt ccgtctggtc aagatagtcg gagattttct gttccgactg
    13081 tatatcgcgg tgaaggtctg taatgttact gatccggtca gcgcacttga gcagcagggc
    13141 atcccgtgac ccgtattcaa gaaggcgctg caggtactgc tccttcgtct catcctttct
    13201 cttggtaacc tccagcacaa gctgtacaac attggtgcca tcactgtcaa tacaccttat
    13261 ctcttcaacc tgggtctcag gaagatcttc aagcagatca tgcagcaggg aggccttgag
    13321 caagacggtg tcagtaaaga acttgtagtc aagcaatata cccagcgtag aaaaacagtg
    13381 cctgaactgg ttgccaccca cagccctctg tacgcttatc agggcagtgg ccttcagaat
    13441 ataaggcgcc agcacaaggt tcttcaatgt ctcaagggca ctcatggtaa aggttgcaaa
    13501 gccacaaagt taacaattcc ggagattcca atctgttatt atgtcataca aattataaac
    13561 agtctctgaa caatgataat acctgtgaaa aacagatagg tgacaataat tagatgagat
    13621 cggagaacac tatccctcca ttctgagaag agtcagcatt ctgcggagtg aattcatgga
    13681 gtcagttttt ttcctgaaaa cagataaaac aaccggccgg agcctatgtt tagtttttct
    13741 gacacctctc agtttaatga aaatgttacc tttgtttatg aaaactcaaa atggtatgat
    13801 tttgacacgt gttctgccag catttgtact gataataacc atggttgccc ccggagttgt
    13861 cattgcacag gtacctgtgg aggtgtcaac tgagaaggtg gtctctggcg gcagggttta
    13921 ttacatgcat gaggttcaga agaaccagac tctctattcc attgcgaagg cttacagagt
    13981 atccgttgat gacctgtcac gtgagaatgt cattccccca aacgggattc aggcagggca
    14041 gatgctgaag gtacccgcac ccgatcagga gggctcggcc acatcgcagt cgcgtccccc
    14101 ggctcagcag gctgctgctg tcacccagcc acgtcctgca gtgcagcctt cgcgtccggc
    14161 gattgccagc ggcgttcccg ggatagatat ttcaggtgaa aagattgttt cgggcggaaa
    14221 gacttattac atgcatgagg tgcagaaggg ccagactctc tattcgattg ccagggctta
    14281 ccgggttacc atcctggata ttgaccgcga gaactccatc ccgccgggag gcatacaaac
    14341 cggccaggtt ctgaagatac cggcatcatt ggcgtttgag gtgattgagg agcagacacc
    14401 tcctgcagat gctgctgcca cgcctgtcag gggcactgca actgcgacaa atgcaccccg
    14461 tggcaatact gctgcagacg caaagatacc agggcaggcg acagctcgcc agcaaacccg
    14521 gatgcccttc caggagcagc ctgctgccag gagtgatacc gaaaaaccgg tagttgacga
    14581 tgacatgact gttaaaacca cggaagtgaa agagaatgcg gatgtaaaga cccagactgc
    14641 agcaaaggcg cagcctgaac cagaaatgca gcagcaggct cagcctgagg taaaaacgga
    14701 gcagcatgct cagcctgagg cagaaacgcg ccaacaggct cagcagcaga caccttcaac
    14761 cggtaagagt gatgatcagc caaagcccgt ccagcc
//
LOCUS       DAOPBK010000053        14706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4121_length_14706_cov_4.415535, whole genome shotgun sequence.
ACCESSION   DAOPBK010000053 DAOPBK010000000
VERSION     DAOPBK010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14706
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4121_length_14706_cov_4.415535"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PLA17_04605"
     CDS             <1..1184
                     /locus_tag="PLA17_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84728.1"
                     /translation="VQNVTIVLGLRAFDSAILPEQAIGRGLRLMRDISPDRTQTLEVI
                     GNDNFEKIVKQLELEGVGINTTNTPPPLPVTIAPEKKRLPYDIEIPATDFSYTRAYKN
                     LSGINGKIFPSLFSSSKLSEDRKTLLKMEVMNLGVEVHQIAVESEFLELGRDLTSYIV
                     SQVMKKAGLTDCFQFVYPICEDYILNKCFEVKIDDIEKEELRKVLRELSIQEAIIELL
                     ATEIGKATVEKKQIKVKGKNIKLSDTPKFTWRRGHIRLKKTVFNFVAIYNPFEAEFAE
                     FLDNASDIEAFAALADIFRIDYLSNSGAIRYYFPDFVAVQLVGKKKVFWILETKGREY
                     PDLDNKNKAIERWCKAVSEQTGYEWKYLLVKQVIFNVDKKSTKTLKQLLDKINSQAKI
                     E"
     gene            1391..1747
                     /locus_tag="PLA17_04610"
     CDS             1391..1747
                     /locus_tag="PLA17_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84729.1"
                     /translation="MKKLKLGLMVILSVCMIVGMESCKRCKGEDPSARVVNNGTSVAS
                     VQIKTSGGFTVNINNVDPGTASPYSNYAPGEITFTITVDSKEYVKTVQMDQCYNYDIV
                     IDKDNNITSTSIERTR"
     gene            2166..3038
                     /locus_tag="PLA17_04615"
     CDS             2166..3038
                     /locus_tag="PLA17_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84730.1"
                     /translation="MEKCYLSYKTKFVITILTVSMAFLFTSCYSNYLTIDYEVHWGAV
                     WNDKHTKVAFVASKRAYRNAEGIAAFPDGGRSLYLLEDVGLYVFDYENKLLDELITFS
                     DLTSWLGTYRSGWHVTLALTDTMVYYLLSPVPGWDWQTEQARTPENLQYITSLKERYQ
                     QAYAFDMYTQNDKVVDSTAFNKLIIKSEKCDLTSLYNQLEKIPLTDWDLKPAEIFPKS
                     DEEYIEETIYLRNPSAKTRRAVVEQIIARKSKAEIESLIKKMDNYKNSLEGSEKIMYE
                     LKSKDTYEQIKALL"
     gene            3013..4056
                     /locus_tag="PLA17_04620"
     CDS             3013..4056
                     /locus_tag="PLA17_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84731.1"
                     /translation="MNRLKHCCKVACLIVITICFASNGWAQSESEWEDFYFKVCDYDY
                     EDMPIDPYPESNIFDGDIKTCWVCGSYKENNPESVHLKMPHGAKVYVGIFTGYGKSQA
                     LFYQNARPKKIRLSVYATVHPEGHVSEIADGYKGLKFPREQIITLADSFGIQKFELDF
                     SRKELNEFSKEVYRQARSYFESPILDSCFFVKIDILESLPGTKYDDICISELYLTGEK
                     CTLNSSTPLTPIKNVYTNQAENSILIDDDQNQGIVIYHDSLSVLQIIEVSANNKWAIV
                     ISMPAEVGEGRVETLYQLVNIPDRKIVNNEIEECTGNYLSGNELAFTTNASGQLMLTY
                     RANDDEYYSIVLK"
     gene            4370..5635
                     /locus_tag="PLA17_04625"
     CDS             4370..5635
                     /locus_tag="PLA17_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84732.1"
                     /translation="MKNQRNTCLEKFKNQLSLLAILFLALPFIVVSCDAIEDIDPIAT
                     ADEYEPNNQLAEAYAIALDTKYNAKISEVTDDDWYKITPSHGSNTYDKVQISVTNVSA
                     DLFIHLELYGNDGKSLATHGATTKGQSLIYTVATPGIAFYIRLSGWDGYVNDHQSRGS
                     YSFTVSNMDANDEYAPNHTIETAKPLAFGNSYNGVIVSKAEDDYYKFENPKPGTWNSY
                     TFNLTNVSEDLKVRIRIYGADKSELCSKGAGTAGADLSYTFVSKENVFYLQVLGWDGY
                     INDHCSSGSYTLTPVNNGNDDYEPDDTFDDARPITSYPTGDLTGTILADAVNDNGGDY
                     EFFKVSIANNKKVSWSVKPVAANTELHFHVYEANREYKGYADGADGQTITGSMNNKTG
                     VNSYFYIKLGAYVGDNGNYTISFTETDAD"
     gene            5658..6476
                     /locus_tag="PLA17_04630"
     CDS             5658..6476
                     /locus_tag="PLA17_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84733.1"
                     /translation="MKRLVIISTLIFIATITAFSQAENVAKEVLNAYKNKDVELLKKN
                     ASGILKMAITKAYFDDKSIQEDARTVDRWDGTIKEVRYASDNIMGRKTVMATAYYADG
                     GKDQIYAVLLSSMDNGKNWVMVGSGLVSLEKKEFEQLSKTIPADGAKKGFSVNWPKKE
                     SKADKAIEESETESNLSVELANGDTFDKISEAKLVECINSLDDDNFFIILNNKENFIQ
                     AAFSDGNFVVEYKEDGQQFAAEKVLTKEETIQLFKDYYQGKDNWKTEIVWKIME"
     gene            complement(6576..6650)
                     /locus_tag="PLA17_04635"
     tRNA            complement(6576..6650)
                     /locus_tag="PLA17_04635"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6613..6615),aa:Val,seq:gac)
     gene            complement(6742..8265)
                     /locus_tag="PLA17_04640"
     CDS             complement(6742..8265)
                     /locus_tag="PLA17_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK84734.1"
                     /translation="MTTLSFGNFFKRILVGTAFNRYRQVFDRAGYAIMLVDNATNHFI
                     KANRAASELFGYTEDEFRKMTPLELSTETCEEPTRSGEKTEESLIMPARRKDGKIITI
                     KVQTSFLEKFRISLISDVTEEYRIRRALELNEERLREAQRIGRFGWWEYRPETNRYIG
                     SEEISRIYNDELSGHSLTYHDIVAYTHPEDRSYFINALEDALEKKKESYSLTYRIITP
                     NGHVKAIAVNSNVKYDENGRLLYRYGTCQDITETQKIMDDLIAARRQAEESDRLKTTF
                     LANFSHEIRTPLNAMLGFLNILTSLDTTEKEKEEMVRLIEINSQRLLRLINDMIDMSR
                     IESNTMEIKYTNIEINGFVNSMVSSFNHELEASGKKDLRLVPVVDKTKTAVYQYIDEL
                     RLSQIFSNLISNAIKFTDQGEIKYGYRISRSDNRYSIVYFVIDNGQGIRLDRLDDIFQ
                     PFVQYWNKDTPKVQGAGLGLAICKRLAELMKGRIWVESQPGKGSSFLFSFNTPVMIE"
     gene            complement(8326..10821)
                     /locus_tag="PLA17_04645"
     CDS             complement(8326..10821)
                     /locus_tag="PLA17_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPK84735.1"
                     /translation="MPSFRAPLSTVVEADDGTLLGARVAADGQWRFPPPDSLPEKYIT
                     CLINYEDRWFRWHPGVNPVAVIKALSSNIRAGKTVRGGSTITMQVARMARGNPERTYG
                     GKLIEMFSAVKLEFFRSKKTILKLYASNAPFGGNTVGIEAAAWRYTGRGTADLTWAEA
                     AMLAVLPNAPALIYPGRHSERLKEKRDRLLLTLVERGKIDSLTCELAMGEPLPEAPLP
                     MPSLAPHLTGKFWLEAPGTRNRTNIDPLLQREASELVNRHVSSLEGNGIHNAAAVVVE
                     VSTGNVVAWVGNSTLPDTTGRHGRDVDMITAPRSTGSIMKPFLYAGLLSSGMLLPNAL
                     IPDIPTRFQGFRPQNADFSFSGAVPAGDALARSLNIPAVRMLQMYEEERFLLLLHQMG
                     FTTFTKPASHYGLSLILGGGEATLLELASAYTAMARTLKNSHKPTAGDQQVESAPVSE
                     TESNIQSNRTQRGVHKSDPSRREQDQPIVRSVRSQRTIHESDPASREQDQSRTRSARS
                     QRTVNVSDPATPEKNQLRSNLSEQQLVNTPLTPASIWLTYEALRRVNRPETETGWQYF
                     GSAPEVAWKTGTSFGYRDAWAIGTTPRHVIAVWVGNADGEGRPGLSGIASAAPLLFDI
                     IRLLPPSSSWFARPDEGMTLVEVCASSGYRAGPDCTERRFEWVPEAGLRSEACPYHTI
                     VSLDPSETWRVNSSCAAPGEMVARSWFVLPPAMEYYYRMRNPSYRTLPPFRKGCSDDM
                     QLPSMEFIYPSRDARIFIPRSLQGQLMSMLPEIAHRRRNATVYWHLDNKYIGMTRHIH
                     QTEIRVGEGEHLITAVDNEGMTVSRKFYCIGTF"
     gene            complement(11526..>14706)
                     /locus_tag="PLA17_04650"
     CDS             complement(11526..>14706)
                     /locus_tag="PLA17_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPK84736.1"
                     /translation="FESQTIFDGNINSSGEASVSFSPDEQLQAPGMLNAIFTTRVFEK
                     GGDASITQTVVPYAPYPAFVGMNIPALGATGRIIYTDRPNEVRLVTLDKNGKPVRSEV
                     EINVYKLDYRWWWESDDEYLGSYISGQRNANVFSETITTSGGEGKTSFSISRENWGRY
                     LVRATLPSGHSTGIIVLVDWPWDYGMKPGGNDGATLLQLNTDKEKYSVGDEISLTFPA
                     PENGRAIVTIENATGLLDVTEMKTRAGNTEVKIKATPAMSPNAYLYVTMLQPHAQTVN
                     DAPVRLYGVIPVMVEDPATKLLPVITMPDKIRSQQEVEIRVEEKNRQEMTYTLAVVDE
                     GLLDLTGFRTPDPWKWFFVKHALGVHTWDLYDIVFGAFGGKLGRGFATGGSEAAIDQS
                     KNRERRFEPVVRFIGPFTLAPGKKASHKITLPQYTGSVRVMVTAAGKANSFGSAEKTV
                     TVADPLMILATAPRVLSPGDRVALPVTVFAQEKNISDVSVSATANDMITLTESTGAVT
                     FSEPGDKDLELMFSTANRTGTAKIDVSAEGRGEKASYSLTVPVRSPNPPDRRSETKSI
                     AKGGKFEKSFSPFGLAGTSAVSVEIFTLPSVNLSKRLKYLTSFPHGCSEQITSGAFPQ
                     VYLPDLLGSGLDNPEIVKNNVQMALRTLSARQIASGAVAQWPGSTYPDDWVTSYVGHF
                     AVEAQKAGYTIPAGFLTKWTSYQRKQASAWRYQPQYRYTANDQAYRLFTLALAGSPDK
                     GAMNRMREIADLPSLARWFLAAAYATTGRVEVAQTLIDVRNLKTEDEYVYYYYGSSLR
                     DQSIILYTLTQMDNKDESLRLLKEVAGAMSRDSWYSTQTTAWGLFAYMNYAKKMKSDG
                     SKPLKATLEFNGNREKLSAAGGAILKNFTPAASNRLVVENESDNPLFVTITEEGVPSV
                     ADMTVKENNLAMKVSYLNMESEPVDVSSLAQGTSFIMAVDITNTTFRTVGNLALTQMV
                     PSGWEIQNTRLFEAQLQLKEGAYEYRDYRDDRVYTYFTLRSGETKRFFIILTASYRGS
                     YSMPAVVCEALYDESFFARRPGFAVEVTR"
BASE COUNT         3902 a         3433 c         3564 g         3807 t
ORIGIN      
        1 atgttcagaa tgtaaccatt gttttgggtt tgcgtgcttt tgattctgcg atactcccgg
       61 aacaggcaat tggcagaggt ttgcgtttaa tgagagatat cagtcctgac cgcacccaaa
      121 cgctggaagt aattggaaat gacaactttg agaagattgt aaagcaattg gaactcgaag
      181 gagtaggcat taatacaaca aacacaccac cgccattgcc tgtaactatt gctcctgaga
      241 aaaagagact accttatgac attgaaattc cggcaactga cttttcatat accagggcat
      301 ataagaacct ttcaggaatt aacggcaaaa tatttccatc gttgttttca tcttcaaaat
      361 taagcgaaga caggaaaacg cttttaaaaa tggaagtgat gaacctaggt gttgaagttc
      421 accagatagc cgttgaatct gagtttctcg aattaggcag agaccttact tcttacattg
      481 tttcgcaggt gatgaagaaa gccggcttga cagattgctt tcagtttgtt tatccgattt
      541 gtgaagacta tattctaaat aaatgttttg aagttaaaat tgatgatatc gaaaaagaag
      601 aattaagaaa agttctgcga gaactctcca ttcaggaggc tatcatcgaa ctattggcga
      661 cagaaatagg gaaagctaca gttgagaaaa aacagataaa agtcaaagga aaaaacataa
      721 aactatcaga cacacctaaa ttcacttgga ggcggggtca catacggcta aagaaaaccg
      781 tttttaattt cgttgcaatt tacaacccgt ttgaagctga atttgcagaa ttccttgata
      841 atgcaagcga cattgaagct tttgctgcgt tggcagacat attcagaata gattatcttt
      901 caaattccgg agcaatccgt tattattttc ccgattttgt ggcagtacaa ttagttggta
      961 agaaaaaggt gttctggatt ttagaaacta aaggtcgtga ataccccgat cttgacaaca
     1021 agaacaaagc gatagagcgc tggtgcaaag cagtatcaga acagacaggt tacgaatgga
     1081 aatatttgtt agttaaacaa gtaatattca atgtggataa aaaatcaacg aaaactttaa
     1141 agcagttatt agataaaatt aatagtcagg caaaaattga ataagtcaat acatctgcaa
     1201 gcacatttaa aagctccgct agccaccgct aactatttca aaaccagaca gaaataacac
     1261 ttcccgcccc aaaaatgatt tggatattga aactattatt gtgaattctt cgttagtcag
     1321 atgagaagaa ccatttgaaa aatggaatta gtcacataaa gtgacagagt atgaatcaaa
     1381 aatttaaaaa atgaaaaaat tgaaattagg attgatggtt atcttgtctg tatgcatgat
     1441 tgttggcatg gagagttgta aaaggtgtaa aggagaggat cccagcgcca gggttgttaa
     1501 taatggaacc agtgtagcca gtgtacagat taaaacgtcc ggggggttca cggtaaatat
     1561 caataatgtt gatccaggca ctgcttcacc ttatagcaac tatgccccgg gagagattac
     1621 atttacaatt acagttgaca gcaaggaata tgtgaagaca gttcaaatgg accaatgtta
     1681 taattatgac attgtcattg acaaggataa taatataaca tcaacgtcaa tagaaagaac
     1741 aagataagac aacagagagc ctgccctgtg gtggcttgcc aggaggaata aaccaaaaag
     1801 agggtgtctc aaaaggggca ccctctttta ttcttttctc cgtaaggcgt actggaatca
     1861 taccagtgac ctcctgcctt tcaggcatta atctctcttc ttctttcact ctatttcttc
     1921 caaaactctg atattgctaa aaataaggtg attagaacat gaattatatg atcctgaaag
     1981 gttgcaaaag gaattttgtt taacttgtag cgtagtttga atgtaaaaag tcagtcagca
     2041 gtaatattca ctgcaagtgg caggcggata acacaatcta cagtgtgacc accgaagcat
     2101 tgctgcaata agggtcacaa gaaactggct ttcgtacgtc agctgccaga attgatataa
     2161 acacaatgga aaagtgttat ttgtcttata aaaccaaatt tgttattacg atactcacag
     2221 taagtatggc atttttattt acatcgtgct attccaatta tttgacaatt gattatgaag
     2281 tccattgggg cgctgtttgg aacgataaac atacgaaagt ggcttttgta gcatcaaaaa
     2341 gagcctaccg taatgcagag ggtattgccg ctttccccga tgggggaaga tccctgtatt
     2401 tattggagga tgttggactt tatgtttttg attacgaaaa caaattactc gatgagctga
     2461 ttacctttag tgacttaacc agctggctgg gcacttatag gtcagggtgg catgtaacat
     2521 tggcactgac tgataccatg gtttattacc tcctgtcacc ggttccgggc tgggactggc
     2581 aaacagaaca agcccgaact cccgaaaact tgcagtatat aacatcattg aaagaaagat
     2641 atcagcaggc ttatgcattt gatatgtata cccaaaacga taaggttgtt gattccacgg
     2701 cgttcaataa gcttatcatc aaatcagaaa aatgcgatct gacttcgcta tataatcaac
     2761 ttgagaaaat tcctttgacg gattgggacc tgaaacccgc tgaaatattt cctaaatctg
     2821 acgaggagta tattgaagaa acgatttacc tgcgaaatcc gtcagccaaa acccgacggg
     2881 ctgtagtaga gcaaataata gcaagaaaga gtaaggctga gattgagtcg ttaatcaaaa
     2941 aaatggataa ttataaaaat agtcttgaag ggtctgagaa gatcatgtac gaactgaaat
     3001 caaaagatac ctatgaacag attaaagcat tgttgtaaag tagcctgcct tattgtaatt
     3061 actatttgtt ttgcaagtaa cggatgggca caatcggaaa gtgaatggga agatttttat
     3121 tttaaagtct gtgattacga ttatgaagat atgcccattg atccataccc tgaatcaaac
     3181 atatttgatg gtgatattaa aacctgctgg gtatgtggct catataaaga aaataaccct
     3241 gaaagtgtgc atttaaagat gccccatggc gctaaggtat atgttggtat ttttaccgga
     3301 tacggaaaga gccaggcgct gttttatcaa aatgcccgcc ccaaaaaaat acgattatcg
     3361 gtttatgcaa ctgttcatcc cgaaggacat gtttctgaaa ttgccgatgg atacaaaggg
     3421 cttaaattcc cgcgcgaaca aattataact ttggctgata gttttggtat tcaaaaattt
     3481 gaattagatt tttcgcgaaa agaactgaat gaattcagca aggaggtata tcggcaggca
     3541 cggtcatatt ttgagtcacc catactcgat tcctgttttt ttgtaaaaat cgatatatta
     3601 gaatcattgc cggggactaa atatgatgac atctgtatct cggaactcta cttaaccgga
     3661 gaaaagtgca cactaaatag ctcaactcca ttgacaccaa taaaaaatgt ttatacaaac
     3721 caggccgaaa acagcatttt aatagatgac gatcaaaacc agggtatagt aatttatcac
     3781 gattcactgt cggttttaca aatcatcgaa gtatcagcca ataataaatg ggccattgta
     3841 atatcgatgc cggctgaggt tggagaggga agggttgaaa cactttatca gctcgtaaac
     3901 atacctgacc ggaaaattgt caataatgag atagaagaat gcactggtaa ttacctgtcg
     3961 ggcaacgaat tagcttttac aaccaatgcc agtgggcagc taatgttaac atacagggct
     4021 aatgatgacg aatattacag catcgtgcta aaatgaaaac gattgtaata cgtattacat
     4081 attaaaacta attttactgc ttttgcagct ctgccccgta ccaggttttg taacggcaga
     4141 atgcgccgtc atccacaatc tacaataatt tcatacatct gtgcgttaag tattaagcac
     4201 gcagctgttt acaactctat cgtaaacaaa ctgtgaatgg catgttgaat atgtatgaac
     4261 ttaaaaaaat attacttcaa gaaacttcaa gtctcctaaa aactgaccct gattgtattt
     4321 ccagaaatca taataatgaa ttcacatttg tttaattaaa ttttttgtca tgaaaaatca
     4381 aagaaacact tgtcttgaga agttcaaaaa tcagttgtca ttattggcaa ttttattttt
     4441 ggcactacca tttattgtgg tttcctgtga tgctattgaa gatattgatc ctatagccac
     4501 agctgatgag tatgagccta acaatcaact ggctgaagca tacgcgattg ctctggacac
     4561 taagtacaac gctaagatta gtgaagtgac agatgatgat tggtataaga ttacgccttc
     4621 acatggcagt aatacttatg ataaggtgca gattagcgta accaatgtat ctgctgattt
     4681 atttattcac ctggagcttt acggtaatga tggcaaaagc cttgcaacac atggcgctac
     4741 aacaaaagga cagagtctga tttatacggt tgccacaccc gggattgctt tttatattcg
     4801 ccttagtggt tgggacgggt atgtaaatga ccatcaatca cgtggcagtt actcctttac
     4861 tgtttccaat atggatgcca acgatgagta tgcaccaaac catactattg aaacggcgaa
     4921 acctcttgca ttcgggaact cctacaacgg ggtaattgta tcgaaagctg aagatgatta
     4981 ttataaattt gagaatccta aacccggcac atggaattcc tatacattta atttaaccaa
     5041 tgtatccgaa gacttaaaag taagaattcg catttacgga gccgacaagt cagaattgtg
     5101 ttccaaagga gctggtactg caggagcaga tctttcatac acctttgttt ccaaggagaa
     5161 tgtgttttat ctgcaagtac tgggttggga tgggtatatc aatgaccact gctcaagtgg
     5221 tagttataca ttgactccgg tgaataacgg aaatgatgac tatgagccag atgacacatt
     5281 tgatgatgca aggccaatca cttcataccc aaccggtgac ctgacaggta caattttagc
     5341 agatgctgtc aatgataatg gtggagatta tgaattcttc aaagtctcga tagctaataa
     5401 taaaaaagta tcatggtcgg ttaaaccggt agctgcaaat acggaactgc attttcatgt
     5461 ttacgaagca aacagagaat ataaaggata tgcagatggt gcagacggtc agacaataac
     5521 tggttcaatg aataataaaa ccggagtgaa ttcctatttc tatattaaac tcggtgctta
     5581 tgttggcgat aacggcaact ataccatttc ttttactgaa actgatgcgg attaataatt
     5641 aatttaaaat agttgtcatg aaaagactcg taattatttc aactttaatc tttattgcta
     5701 ctattactgc cttttcccag gcagagaatg tagctaaaga ggtgttaaac gcatacaaaa
     5761 acaaggatgt tgaattgctc aaaaagaacg cctccggaat tctgaaaatg gcaatcacga
     5821 aagcttattt cgatgataag agcattcagg aagatgccag gacagtcgat agatgggatg
     5881 gcactataaa agaggtgcgc tatgcaagcg ataatataat gggccgaaaa acagttatgg
     5941 ctacagcata ttacgctgac ggggggaaag accagattta tgctgtcctt ttatcaagca
     6001 tggataacgg gaaaaattgg gttatggtag gaagtggcct ggttagttta gaaaagaaag
     6061 aatttgaaca gttaagcaaa accattcctg ccgatggggc gaaaaaggga tttagcgtca
     6121 attggccgaa aaaggaatca aaggccgata aggcaataga agaatcagaa acagagtcaa
     6181 atctctcagt cgagctggcc aatggtgata cctttgataa gataagtgaa gcaaaattgg
     6241 tggaatgtat caacagcctt gatgatgata atttctttat aattctgaat aacaaggaaa
     6301 actttattca agccgcattt tctgatggta attttgttgt tgaatataaa gaagatgggc
     6361 aacaatttgc tgcagaaaag gtgctcacaa aagaagagac catccaatta tttaaagatt
     6421 attatcaggg aaaagataac tggaaaacag aaattgtctg gaaaataatg gagtagactt
     6481 ttaattggtc aatccttaaa atcatcaact gtttttgacc atattaccca aaaaagggtg
     6541 tctcaaaaga ggcacccttt ttaatacttt ctccgtgggg cgtactggaa tcgaaccagt
     6601 gacctcttgc ctgtcaagca agcgctctaa accaactgag ctaacgtccc gttatgcctt
     6661 ccgccggcag gcggctatct ataaccagct gagccgccgg cccgggcggg aacaaagata
     6721 aacaaaattt gagttctgca attactcgat catcacgggc gtgttgaacg agaagaggaa
     6781 cgacgatccc ttgccgggct gcgactcaac ccagatgcgt cccttcatca gctcggcaag
     6841 acgcttgcag atggccagac ccaatcctgc accctgcacc ttaggggtat ccttattcca
     6901 gtactgtaca aacggctgga aaatatcatc aagcctgtca agccttatcc cctggccgtt
     6961 gtcaatgacg aaatagacaa tagagtaacg attatcactc cgggagattc tgtaaccata
     7021 ttttatctcg ccctgatcag tgaatttgat ggcattgctg ataaggttgc tgaagatctg
     7081 actcaggcgc agttcgtcaa tgtattgata aacagcggtc ttcgtcttgt caacaacagg
     7141 tacaagcctt agatccttct ttcccgaagc ctccagttca tgattgaaag aggagaccat
     7201 tgagttgaca aacccgttta tctcaatatt ggtgtacttg atctccatgg tgttgctctc
     7261 gatgcgtgac atgtcaatca tgtcattgat gagccgaaga agcctctggg agttgatctc
     7321 gatgagcctc accatctcct ccttctcctt ttcggtagtg tcaagcgaag taaggatgtt
     7381 gaggaacccc agcatcgcat tgagaggtgt acgtatctcg tggctgaagt tggcaaggaa
     7441 tgttgtcttg agcctgtcgg actcctcggc ctgtcgacgt gcggcaatga ggtcgtccat
     7501 tattttctgt gtctcggtga tgtcctggca ggtaccataa cgatagagca gacgtccgtt
     7561 ttcgtcatat ttgacgttag agttgacggc aatggccttc acatgcccgt taggagttat
     7621 aattctgtag gtgagagaat aagactcctt ctttttttcg agggcatcct ccagtgcatt
     7681 tataaagtat gatctgtcct ccgggtgagt gtaggctaca atgtcatggt aagtgagcga
     7741 gtgacctgaa agttcgtcgt tataaatcct cgatatttct tccgacccta tatacctgtt
     7801 tgtctccgga cggtattccc accatccgaa ccggcctatc cgctgtgctt ccctgaggcg
     7861 ttcctcattc agctcaaggg cccggcgtat ccggtactct tcggtaacat cgctgataag
     7921 gcttatccgg aatttttcca ggaaacttgt ctgcaccttg atggtaatga ttttaccatc
     7981 cttgcgcctt gccggcatga tcagtgattc ttcggttttc tcacccgagc gggtcggttc
     8041 ttcacaggtt tctgttgaga gttcaagcgg agtcatcttc cgaaactcat cttcggtgta
     8101 tccgaacagc tcagatgccg ccctgtttgc cttgataaaa tggtttgtag cgttatcaac
     8161 aagcataatt gcatatcccg ccctgtcaaa aacctgcctg taacggttga aggcagttcc
     8221 cacgaggata cgtttaaaga aattcccgaa tgaaagtgtg gtcacctttc cttaaagttg
     8281 gcttgttatg cgatggtaaa aatagttctt tttccggatt tgccgtcaaa atgttccgat
     8341 gcagtaaaac ttgcgtgaaa cggtcatccc ttcattgtcc acggctgtta tgagatgctc
     8401 gccctcacct accctgatct ctgtctggtg aatatgcctt gtcatcccga tatatttgtt
     8461 gtcaagatgc cagtaaacgg ttgcgttccg cctgcggtgg gcaatttccg gcagcattga
     8521 catgagttgt ccctggaggc tgcgggggat gaaaatgcgc gcatcgcgcg aagggtagat
     8581 aaactccatc gagggcagct gcatatcatc agaacaccct ttgcgaaagg gtggaagcgt
     8641 acggtatgac ggattacgca tgcgatagta atactccatt gccggcggaa ggacgaacca
     8701 tgaccgggca accatctctc ccggtgcggc acaggaactg ttgacccgcc atgtctctga
     8761 cggatcgagg ctgacaattg tatgatacgg gcaagcttcg ctgcgaagtc ctgcctcagg
     8821 aacccactca aacctcctct cagtgcagtc gggaccagcc ctgtatccgc ttgaggcaca
     8881 tacctcgacg agggtcattc cctcatcagg tctcgcgaac caggatgatg acggtgggag
     8941 gagccttatg atgtcaaaga ggaggggtgc tgccgaggcg atacccgaca atccgggacg
     9001 accctcgccg tcagcattgc ccacccacac cgcgatgaca tgccggggtg ttgtgcctat
     9061 ggcccacgca tcgcgatagc cgaagctggt accggttttc catgccacct ccggcgcact
     9121 gccgaagtac tgccacccgg tctctgtctc gggcctgttg acccgccgaa gggcttcata
     9181 ggtcagccag atggatgcag gagttagagg ggtgtttaca agttgctgtt ctgacaggtt
     9241 ggatcggagt tgattcttct ctggtgttgc aggatcagat acattaactg tgcgttgcga
     9301 ccttgcactt cttgtgcggg attgatcctg ttcccgacta gcaggatcag attcatgaat
     9361 tgtgcgttgc gacctgacgc tgcgtacgat gggttgatcc tgttcccgac gcgaagggtc
     9421 ggatttatgt actccacgtt gagttctgtt actttgtata ttggattcag tctctgacac
     9481 cggagcagac tcaacctgct gatcgccggc agttggctta tgactattct ttagggtgcg
     9541 cgccattgcc gtatatgctg atgccagctc cagcaacgtg gcctcgccgc cgccgagtat
     9601 gagggagagg ccgtagtggg aggcgggttt ggtgaaggtg gtgaagccca tctggtgcag
     9661 gaggagcagg aaccgttcct cttcgtacat ctggagcatg cgtacggcgg gtatgttgag
     9721 tgagcgggcc agcgcgtcac cggcaggcac ggcgccgctg aaggagaagt cggcgttctg
     9781 ggggcggaag ccctggaagc gggtggggat atcgggtatg agtgcgttag gaaggagcat
     9841 gcccgaggag aggaggcctg catagagaaa cggtttcatg atgctgccgg tgctgcgggg
     9901 cgccgtgatc atgtcaacat cgcggccgtg cctgccggtg gtgtcaggga gggtgctgtt
     9961 acccacccag gcaacgacgt tgccggtgga gacctcaact accacggcgg cggcgttgtg
    10021 gataccattc ccctccaggg aggagacatg cctgttgacc agttccgatg cctcgcgctg
    10081 gagcaaaggg tcaatattgg ttcggtttct tgtaccgggc gcctcaagcc agaactttcc
    10141 tgtgagatgc ggcgccagcg acggcatggg aagaggagcc tccggcaacg gttcacccat
    10201 tgccagctcg catgtcaggc tgtcaatctt ccctctctcc accagggtca gcagcagcct
    10261 gtctctcttc tctttcagcc gttcggagtg gcgtccggga tagatgaggg caggggcatt
    10321 gggcagcaca gccagcatgg ctgcctctgc ccatgtcagg tcggccgtgc cgcgaccggt
    10381 gtacctccag gcagcagcct cgatgccgac agtgtttccg ccgaaagggg cattggaggc
    10441 atagagcttg aggatagttt tttttgagcg gaagaattcc agcttgaccg ctgagaacat
    10501 ttcaatgagc ttgccgccat aggtgcgttc cgggttgccg cgtgccatcc tcgccacctg
    10561 catggtgatg gtgctgccgc cccggaccgt tttgcctgcc ctgatattac tggacagtgc
    10621 ttttatgacc gcaacgggat tgacccccgg gtgccaccgg aaccagcggt cctcgtagtt
    10681 gatgaggcag gtgatatatt tttccggcag gctgtcgggc ggggggaatc gccactggcc
    10741 gtcggcagcc acacgggcgc ccaggagggt gccgtcatca gcctccacca cggtagagag
    10801 gggtgcacgg aatgatggca tcggcgccac aaggatggca agaagaagga cggcgagtcc
    10861 gtagattaca atgctttatg gcctgtggcc agttctgcgg tattccatct ctatatctgc
    10921 aatatcataa ttgctataat atataaggta cagcaattgc tggcttccga ctaatactgt
    10981 tgaccgatgc tgcagaccgg aacctttgac cagtactgct atccattgcc gtcgatctgt
    11041 acagtcaacc tgtgcttgcc aactgctagt ccctgaccac tggcctctga ctgctggctg
    11101 ctgaccagtt ctgctgatcg ctggcagtca accagaacag ttgacacttt gtttcctgcc
    11161 agttctgcca actgctggca gccagccgga gctccagacc ggacttgccg gtcagtagtg
    11221 tcaaccactg gctccgaccc ttagccattg ccccccggcc ccgcccggtg ctgttagctg
    11281 atattgccgg ccgctgacct ccgacccctg gccattgacc cccggccctg ccaggtgcta
    11341 ccggtctgtt cggcgccacc acccggcact acccgctggc caccaactgc tactaccaac
    11401 gtcccaacca ctaacttgca ccgcttccca ctaatcgcca gccagcacca tctcccgtct
    11461 cccggcactg ccaaccgctg gtcgccaatt ggtcctgtca atttctagct tccagccaat
    11521 cctccctatc tcgtaacctc cactgcaaaa cccggccggc gggcgaagaa ggattcatca
    11581 tagagcgcct cacacaccac cgcgggcatg ctgtaactac cacggtacga ggcagtcaga
    11641 atgataaaga acctcttcgt ctcacctgac cgcagagtga aataggtgta aacacggtca
    11701 tccctgtagt cgcggtactc atacgcaccc tccttcaact gcaattgcgc ctcaaagagc
    11761 cgggtgttct gtatctccca tcccgatggc accatctgcg tcagtgccag attacccaca
    11821 gtacggaacg tggtattcgt tatgtcaaca gccattatga aacttgtccc ctgcgccagc
    11881 gagctgacat caacaggttc cgactccata ttgaggtaac tgaccttcat cgcaaggtta
    11941 ttctccttca cggtcatgtc agccacggag ggcacaccct cctcggtgat ggttacaaac
    12001 agtggattgt cgctttcgtt ctcaacaacc agcctgttgg aggcggcagg agtgaagttc
    12061 ttcaagatgg cgccgccggc agcactgagc ttctcacggt tcccgttgaa ctccagcgtc
    12121 gccttgagcg gtttcgatcc atcgctcttc atcttcttcg cgtagttcat atacgcaaac
    12181 aggccccacg ccgtggtctg cgtactatac cagctgtcac ggctcatcgc cccggccacc
    12241 tccttcagca gcctgagcga ttcatcctta ttgtccatct gcgtgagggt atagagtatt
    12301 atcgactgat ccctgagcga gctgccatag taatagtaca catactcatc ttcggtcttc
    12361 aggttacgca cgtcaatcag cgtctgcgcc acctcaaccc gtcccgttgt ggcataggca
    12421 gctgcaagga accatctggc cagcgacggc agatcggcaa tctcgcgcat gcggttcatt
    12481 gcgcccttgt cgggcgaacc ggccagggcc agtgtaaaca gcctgtaagc ctgatcattg
    12541 gctgtatagc gatattgcgg ctgatacctc catgccgagg cctgcttcct ctggtacgag
    12601 gtccatttcg tgaggaaccc tgccgggatg gtatatccgg ctttctgtgc ctcaacggcg
    12661 aagtgtccca cgtatgaggt tacccagtcg tcaggatagg tgctgccggg ccattgtgcc
    12721 acagcccctg aagctatctg cctcgcggag agggtacgca atgccatctg cacgttattt
    12781 ttgacaatct cgggattatc gagcccggag cccagcagat caggcaggta aacctgcggg
    12841 aacgccccgg aggtgatctg ctcggagcag ccgtgcggga acgaagtgag atacttcagg
    12901 cgtttgctca gattcaccga cggcagtgtg aatatctcaa ccgacactgc agatgttcct
    12961 gcaaggccaa acggcgagaa actcttttca aactttcccc ctttggctat cgattttgtc
    13021 tcagacctcc tgtcgggcgg attgggtgag cgtaccggca ctgtcaggct gtacgatgcc
    13081 ttctcgcctc tcccctcggc gctgacgtct atctttgccg ttcccgtgcg gtttgcagtg
    13141 ctgaacatca gctccagatc cttgtcgccc ggctcgctga atgtgacagc gccagtcgac
    13201 tccgtcaggg tgatcatatc attcgccgtg gcggaaacag agacatcaga aatattcttt
    13261 tcctgtgcaa aaacggtcac cggcagagcc accctgtcgc ccggcgagag caccctcggg
    13321 gctgttgcca ggatcatcag cgggtcagcc accgtcactg ttttttcggc tgagccgaag
    13381 ctgttggctt taccggccgc ggtgaccatt accctcaccg atccggtgta ctgtggcagc
    13441 gtgatcttat gagatgcctt ctttcccggt gcgagggtga agggaccgat gaaccttacg
    13501 accggttcga agcgtcgctc cctgttcttg ctctggtcta tggctgcctc acttcctcct
    13561 gtcgcgaatc cgcggcccag tttgcctccg aaagcgccga agacgatgtc atagaggtcc
    13621 catgtatgca ctcccagcgc atgcttgacg aagaaccatt tccacgggtc aggtgtgcgg
    13681 aaccctgtca ggtcaagcag gccctcatca accactgcca gcgtgtatgt catctcctgg
    13741 cggttcttct cctccacgcg tatctctacc tcctgctgtg agcgtatctt gtcaggcatg
    13801 gtgatcaccg gcagaagctt cgtggccggg tcttccacca tcaccgggat gactccgtaa
    13861 agtctcaccg gcgcatcatt gacagtctgc gcgtggggct gcagcatcgt aacatagaga
    13921 taggcattgg gagacatggc cggtgtagcc ttgatcttaa cctcggtgtt gccggcccgg
    13981 gtcttcatct cagtcacgtc aagcagcccg gtggcgttct ctatggttac aatggcgcga
    14041 ccgttctccg gcgccgggaa ggtgagactg atctcatcac ccacgctgta tttctccttg
    14101 tcagtgttga gctgcagaag agtggcgccg tcgttgcctc ccggcttcat gccgtaatcc
    14161 caaggccagt caaccagcac aataatcccg gtggagtgcc ccgaggggag tgttgccctg
    14221 accagatagc gtccccagtt ctccctgctg atgctgaagg aggtcttgcc ttcgccgccg
    14281 gatgtggtta ttgtctcaga gaaaacattg gcgtttcttt gaccggagat ataggaacca
    14341 agatattcat catcagattc ccaccaccag cggtaatcaa gcttatagac attgatctcc
    14401 acctcgctgc ggaccggttt gccgttcttg tcgagggtca cgagcctgac ctcattggga
    14461 cggtcagtat agatgattcg tccggttgct cccagtgcgg gaatattcat cccgacgaag
    14521 gcaggatacg gggcatacgg aaccacggtc tgtgttatgc tggcatcacc gcccttttca
    14581 aacaccctgg tggtgaatat cgcgttgagc atgcccggcg cctgtaactg ttcatcaggg
    14641 gagaaggaga cagaggcttc gccggagctg ttgatgttgc catcgaagat agtctggctc
    14701 tcgaaa
//
LOCUS       DAOPBK010000054        14415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4235_length_14415_cov_5.480084, whole genome shotgun sequence.
ACCESSION   DAOPBK010000054 DAOPBK010000000
VERSION     DAOPBK010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14415
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4235_length_14415_cov_5.480084"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLA17_04655"
     CDS             complement(<1..198)
                     /locus_tag="PLA17_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84737.1"
                     /translation="MKIWVLWRSFFIPTGAAFFMRFAQVRENAGEGIDLKKPLKINGF
                     CFYLGGADGTRTRDPRRDRPVF"
     gene            488..583
                     /locus_tag="PLA17_04660"
     CDS             488..583
                     /locus_tag="PLA17_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPK84738.1"
                     /translation="MKEWQQRPLDPVYYVVWMDGIVVKVRQNGNW"
     gene            complement(658..1932)
                     /locus_tag="PLA17_04665"
     CDS             complement(658..1932)
                     /locus_tag="PLA17_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPK84739.1"
                     /translation="METKNNNRTIHVYAHWTGMKEPLYMGELNSEFTRGKEVFSFNYS
                     ENWLKSSYSQVLDPELHFYSGSQYVSDEKMNFGVFLDSSPDRWGRLLMRRREAAIARA
                     EGRNANTLRESDYLLGVFDGHRMGALRFKDKLDGPFLNDNKDFASPPWTSIRELEQIS
                     LKLEDDNVIENPEYQKWLMMLVNPGSSLGGARPKASVLDTDGHLWIAKFPSKADKKDI
                     GGWEMLTNEIAKNAGINVASSMVQKFSSNHYTFLTKRFDRTSNGERIHYASAMTMLGY
                     VDGTDFKDGASYLEIADFISNHGANVENDLTELWRRIVFNIFVSNTDDHLRNHGFILT
                     EKGWILSPAFDLNPNEDGAGLSLNISLDDNSLNLEIPLEIIDYFRLEKSKALKIIDEI
                     RISVKKWRSIANKYHLSKTEQDIMSYVFDKTI"
     gene            complement(1916..2260)
                     /locus_tag="PLA17_04670"
     CDS             complement(1916..2260)
                     /locus_tag="PLA17_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK84740.1"
                     /translation="MRTKRSILLPKAQKALSTFGENIKLARLRRKYSAEQVAERANIS
                     RPTLLSIERGNPSVSIGAYVKVLFVLGLEKDFAQLAKDDALGRRLQDAKLTIKERAPK
                     IKKTRTTDGDKE"
     gene            3174..>7507
                     /locus_tag="PLA17_04675"
     CDS             3174..>7507
                     /locus_tag="PLA17_04675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPK84741.1"
                     /translation="MDQISFIDTEVDPKTLKILDIGSIRSDGGTFHKNSVAEFISFLD
                     GTKFICGHNILRHDIKYIGKALAEAGIDPSNIIDTLFLSPLLFPSKPYHALLKDDKLQ
                     TDYANNPLNDSIKARDLFYDEITHFNQTEENLKQIYYLLLKERPEFRAFFRLIGYTSD
                     ETNPESLIRRKFENEICEQADLTGMIEGEPVGLAYSLALINSFILYPEKRSVTPPWVL
                     KNYPEVEQIMFRLRNRPCIRGCSYCNKALDIHSGLKRVFGFDSYRTFRGEPLQEQAVR
                     AAINNKSLVAVFPTGGGKSLAFQVPALMSGDNVQGLTVVISPLQSLMKDQVDNLEKAG
                     ITRAVTINGLLDPVERAKSIERVEDGSASLLYISPESLRSITIERLLLGRKIVRFVVD
                     EAHCLSTWGQDFRVDYLYIAEFIKSVQKKKNLQEAIPVSCFTATAKPQVIDDICNYFM
                     GELSLDLERFISPPSRTNLQYKVFEKGSEEEKYQALRDLIEERHCPAIVYVSRTRKAY
                     QLAERLTKDGLNAKPYHGRMDVQEKTANQEAFISGDVPIMVATSAFGMGVDKKDVGMV
                     VHYEISDSLENYIQEAGRAGRDENIVADCFVLYNEEDLNKHFILLNQTKLTIKEIQQV
                     WKAIKDITRFRSSVSNSALEIARKAGWDDRVSEIETRVKTAIAALENAGYLKRGQNMP
                     RVFANSILSRTAQEAIEKIYASTRFNERQKQQAVRIIRRLFSSKSRKHATGEEAESRV
                     DYISDHLGIVKEEVIEIINLLREEKILADAKDLTAFIKSREKRNRSLNIVDTFRQIEN
                     CLVRIFDEEAKMFDLKELNETAEAQGCEGSTVSRIKTVINFWAIKNWIKRKNLEYSKN
                     HVSIVSLYPRELLIERLKKRHELARFIIEFLWERCKADLSEEDVAKEEVLVEFSVLEL
                     KTEYESQQSLFRTEVSPDDIEETLFWLSRIDAIDIEGGFLVTYNRLTIERLEQDNRKR
                     YTAEDYQNLNRFYDNKAQQIHIVGEYARKMLVDYRDALQFVEDYFRLTYPSFLNKYFR
                     SRQREITRTLTPDKFRQLFGELSPAQLKIINDAETQHIVVAAGPGSGKTRVLVHKLAS
                     LLLVEDVKHEQLLMLTFSRAAATEFKRRLIGLVGNAANFIEIKTFHSYCFDLLGKIGT
                     LEKSDAILKTTVEKIKGREVEASRITKTVLVIDEAQDMSEEEFALIAALIERNEDMRV
                     IAVGDDDQNIFEFRGSSSRYLEHFIRANQAFKHELVENFRSKNNLVEFTNQFVIRIRH
                     RLKNTPIIARQTDNGKIKVVRYLNGTLIVPFVHDLLNTELSGTTCALTRTNDEALQVA
                     GLLLRNGMPARLIQSNDGFNLINLAEIRFFLSQPGLAGNIITVTDEAWDKAKRSLAQR
                     YLRSTKYELCKNLIRAFEAANPKMRYRSDLEVFIRESKLEDFYGENGETIIVSTIHKA
                     KGKEFDNV"
     assembly_gap    7508..7607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7889..9532
                     /locus_tag="PLA17_04680"
     CDS             7889..9532
                     /locus_tag="PLA17_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPK84742.1"
                     /translation="MSEKYSVSPSGEKFPLPKPEDYKAEFEKIKRRVKQERAKGREIV
                     VVMGVGFVGAVMAAIIADTVDKKGEPSKFVIGMQRPSVRSFWKIPLLNRGISPVKAED
                     PEVDPMIARCVREKKTLIATYTYDVLKLADVVVVDVQCDYIKEEFGNVHAGHTDMAAL
                     EASLAIIAEHIPAEALVLIETTVAPGTTEQVAYPIMKKAFRKRGITTDPLLAHSYERV
                     MPGRNYVASIRDFWRVCSGINEEAKNRVVKFLTEVLNTEKYPLTVMDRPIESETAKIV
                     ENSYRATILAFLDEWSLFAERNGVDLIKVIKAIKMRPTHSNMIFPGPGIGGYCLPKDG
                     GLGVWAYKHILGFEDDIFRITPMAIDINDTRGLHVGQLTRDALRNMGKPIAAADVLVL
                     GAAYREDVGDTRYSGSEVVIRKLAEMGAEIRVHDPYVEHWWEFEQQDTYPAEGQSKAQ
                     FFRNQEKLKEIRIEQNLNKALKGAEAVILAVPHSAYLNLDPDKVVKTIGQPAAIIDCF
                     GILTDDKIRRYFQLGCEVKGLGRGHIQRIKEETRKAQEI"
     gene            9563..10414
                     /locus_tag="PLA17_04685"
     CDS             9563..10414
                     /locus_tag="PLA17_04685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPK84743.1"
                     /translation="MKRKVDELYDEYRNGNVSRREFMKKLALVTGGTAAAVVILPGCN
                     GATTRAAAKNAMKVVSEFITYPGASGDMRAWISIPEGKEKYPGVLVIHENRGLVPHIR
                     EVNDRMAMEGFVALAPDALSPLGGTPDDQNKSREMMGQLDREETIRNFVAAVKYLKTH
                     PRTTGKVGCTGFCWGGAMTNQVAVNSPDLDAAVPYYGSTPEPEDVPKIRAKVMAHYAD
                     NDERINAGIPAFEEALKKAGIEYHIFIYEGTQHAFNNDTGERYNKEAADLAWKRTTDF
                     FKEKLKS"
     gene            complement(10411..11550)
                     /locus_tag="PLA17_04690"
     CDS             complement(10411..11550)
                     /locus_tag="PLA17_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HPK84744.1"
                     /translation="MSSESREFQVFVKPVGARCNLRCSYCYYSGHSHPGRESGGQRMS
                     DKVLERYIRQHIQAAGGGELFFSWHGGEPALAGIEFYRRAVALEKKYAPPECRMVNGI
                     QTNATLLDDEWGRFLSEEGFYVGVSLDGPERFHDSSRLRAYGRGTFADVMRGVEILRR
                     HRVPHEVLCVVSNNNVHAPLEVYRFLRDLGIEFVTFLPLVERKSRNSSEVTARSAGAA
                     AFGRFLAEIFDEWIANDIGKIKVQIFEEALRTAFGQEHTLCIFKPVCGAVPAVEMNGD
                     FYSCDHFVDGEHLIGNIMNRSLESLLDDPRQKAFGEAKKTTLPRYCLDCEVLDMCNGE
                     CLRNRFISAPDGEPGLNYLCDGYRYFFNHCRPFINEVARLWRKGS"
     gene            complement(11574..12875)
                     /gene="thrC"
                     /locus_tag="PLA17_04695"
     CDS             complement(11574..12875)
                     /gene="thrC"
                     /locus_tag="PLA17_04695"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPK84745.1"
                     /translation="MKYYNLKNPLVKTGFREAVERGISPDMGLFMPERIPRLSDDFFR
                     RLASMTLPEIGMEVGSLYTGDDIPRSVMRQICGEAFDFPVIIRRLDERKQVLELFHGP
                     TAAFKDFGARFMAGCFRYFSSRDDKRRVVLVATSGDTGGAIASSFLGVEGVDVIILYP
                     SGKVSNLQENQLTTLGRNITAIEVEGSFDDCQAMVKSALSGSANRTNLKLTSANSINI
                     ARLIPQSFYYYYAIGRSGSGMAVSVPGGNFGNITAAAMARRSGLGIHHLVAATNVNRV
                     VPDYLKTGIYTPAESVATIANAMDVGNPGNFVRLRRIFDDDYEAIKDSVSGYWFDDDR
                     IREHIGQEYQRTGYLLDPHSAVASLGLEAYLNQHPGTVGIFVATAHPAKFREIVEPLT
                     GQNVEIPESLRNFLAGKKQSVKMKAGPQNLEEYLDDRYQNE"
     gene            complement(12895..13824)
                     /locus_tag="PLA17_04700"
     CDS             complement(12895..13824)
                     /locus_tag="PLA17_04700"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPK84746.1"
                     /translation="MAGKRIRVKAPATVSNLSCGFDVLGLALDEPFDVVDLELTDTGT
                     VVIDDIRGCGRLSRDPEQNVVGVVLKAASAMAGSSTGFRVSIEKGIRPGSGIGSSSAS
                     AAAAAFGANLLLGEKFTSSEMVRLAMEGERLASGAAHADNVSPALLGGIILVRSYEPL
                     DIVRLHVPPELYCVVIHPETEIKTSVARGLLDRQVPLTTAVRQWGNLAGLVAGLYRED
                     YDLIGRSLIDFVAEPKRSVLIEGFDLVRSTAMECGALGAGISGSGPSVFALCRGGDSA
                     AAVMKGMSEAMKSQGIGFDAYVSPVSRRGAQVW"
     gene            13921..>14415
                     /locus_tag="PLA17_04705"
     CDS             13921..>14415
                     /locus_tag="PLA17_04705"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPK84747.1"
                     /translation="MRLTLQIILLSLLSLQVAGQNFTDSNLPIVIISTDGGVTIPDEP
                     KVKATMKIIDRGPGERNYVTDQDNPLYLNYNGRIGIETRGSSSQESPKKNYGFKTRKE
                     DDKTNNNVSLLGMPVENDWILGGMVFDTAFIRDYYCHNLYGKMGNYASRAVYCEVIIN
                     NNYAG"
BASE COUNT         3969 a         3466 c         3238 g         3642 t
ORIGIN      
        1 gaataccggc ctgtcacgcc gggggtcgcg ggttcgagtc ccgtccgcac cgcccaaata
       61 aaaacagaaa ccgttgattt tcaacggttt ttttaagtca atgccttccc cggcattttc
      121 ccggacttga gcaaacctca taaaaaaagc ggctccggtt gggataaaaa atgatcgcca
      181 taaaacccat attttcaaag aactcctgat ggaatggcaa taatcattca gaatacacaa
      241 cttaattgga cgaagaaaga ggttctcgaa tgttggtatt aatcagttat taaccagatt
      301 tcgtagatgt tcaatgagtt cctcaaaatc aggacattta ccataaatca tactcctctg
      361 catttcctgg taatctaacc gatattgctc catgaattct gctggtggga tgaaatccag
      421 atcattaaga gttaattcag aatatgcgat tcctgataca ggagtgaaga ccgctctgtg
      481 attacatatc aaggaatggc agcagcgtcc ccttgatcct gtctactatg ttgtctggat
      541 ggacggcata gttgttaagg tgaggcagaa tggcaattgg tgacagatgt ggcgttcttt
      601 aaacccggcg gctactcgtt tacacaaaaa ttttcacagg ctcctcctcc agtaaactta
      661 tatcgtttta tcaaaaacat atgacataat gtcctgctcc gtcttagaaa gatggtactt
      721 atttgctata cttctccatt tttttaccga aattcttatt tcatctatta ttttcaatgc
      781 ctttgatttt tcaagacgga agtaatcaat aatctctaaa gggatctcaa gatttaatga
      841 attgtcatca agagaaatat taagtgataa cccggcacca tcctcattcg gattcaaatc
      901 aaatgcaggt gaaagaatcc atcctttctc agtaaggata aatccatgat ttcgaagatg
      961 atcatcagta ttagacacaa aaatattgaa cactattctt ctccataatt cagttaaatc
     1021 gttttccaca tttgcaccgt ggttagatat gaaatctgca atttcgagat agcttgcgcc
     1081 atccttaaaa tctgtgccat caacatagcc cagcatagtc attgctgaag cataatgaat
     1141 tcgttcacca ttagaggttc tgtcaaatct cttagtaaga aatgtataat ggttgcttga
     1201 gaatttttga accattgaag aagcaacatt tataccggca ttttttgcaa tctcatttgt
     1261 aagcatctcc caccctccaa tgtctttctt atctgcttta cttgggaatt tggcaatcca
     1321 aagatggcca tcagtatcaa gtacacttgc ctttggtctt gcaccaccaa gagacgatcc
     1381 cggattaacg agcatcataa gccatttttg gtattcaggg ttttcaatta cattatcatc
     1441 ctctaatttt aagctaattt gttccaactc tcttattgaa gtccatggcg gagaggcaaa
     1501 atctttatta tcattcagga atggcccatc taatttatct ttaaatcgta atgcccccat
     1561 cctatggcca tcaaatactc ctaacaaata atctgattct cttaatgtat tcgcattcct
     1621 gccttctgcc cgtgcaattg ctgcttcacg ccttctcatc aaaagtcgac cccatctgtc
     1681 aggcgatgaa tccagaaaaa ctccaaaatt cattttctca tcacttacat attgtgaccc
     1741 ggaatagaaa tgcagttcag gatcaagaac ctgggagtaa cttgatttta accaattctc
     1801 agaataattg aatgaaaaga cctctttacc cctggtgaac tcagagttca attcgcccat
     1861 gtacaaaggt tctttcatgc ctgtccaatg agcatataca tggatagttc tattgttatt
     1921 ctttgtctcc atctgtggtt cttgttttct ttattttggg ggcccgctct ttgatcgtta
     1981 atttggcatc ctgaagtctc cttccaagag catcatcctt ggccaattgt gcaaagtctt
     2041 tttccaaacc aagaacaaaa aggactttta catatgcccc aatgcttacg cttgggtttc
     2101 ctctctctat agataataat gttggcctgc taatattagc tctttcagct acttgctcag
     2161 cactatattt ccttctaagc cttgcaagtt taatgttttc gccaaaagtc gaaagtgctt
     2221 tctgcgcttt tggcaataat attgatcttt ttgtcctcat aacgtaaatt atattttaca
     2281 aatatacatg tttttgtaaa ttacaattta cattatgaca tttttttccg tattactatt
     2341 cccctttctt aaaaaaatag cgcaaacctc ttttgacata ttttttggaa ctatcaattt
     2401 ctattattca ttttccggta attccaggat attgtcgctt gccccaattt ctttcagttg
     2461 cggtattgac gttcctgcaa taccctttat cgatccaatg aagtggttgg tattaagtaa
     2521 aaccctctcc gattctcttt tctgtattgc ctccgcctct tgttgaagta tttctgatag
     2581 tttctgttca tattttaatg ccagatcctg ctcctggtca aggagtctcc tttttaacta
     2641 cttattttca attttggtag tcttcagttc tgatatttcc ttcttcgctg actcagcctc
     2701 ggatgaaaga gctttcaatg gctgttcata ctgcttttca acctggcttt taatttgtct
     2761 gtttacgttt tccgattcta ccttcagctt ttcagaaacc agttggtcaa tattggatta
     2821 ctgcttttta atgtcagcct ccttttgctt gacggcattt tcttttactg catactcttc
     2881 ctgaatctta gtgatgctct gattcatttc attgcagtac ttttcttcta ttggccgggc
     2941 aagaacatcc ttaacacgcc atgtaaattc atttctctaa aatcacaata agggctcaat
     3001 accaggcata gaaatattct gtactgccaa ttaattcaat taattgaccc ggcaacacaa
     3061 ctacgcaaac agactcccgt tataaaataa accctggaat agtaagggat atataaatat
     3121 tactacaacc gtatcattca taacgttgga aagctcttcg cccggagtaa gcaatggacc
     3181 aaatatcatt catagacacg gaagttgatc cgaaaacctt aaaaatactt gatatcggaa
     3241 gtatcaggag cgatggcggt acattccata aaaactctgt tgctgagttc attagctttc
     3301 ttgacggcac aaaattcata tgcggtcata acattttaag acacgacatt aaatatattg
     3361 gcaaggcatt ggccgaagcg ggaatagacc cttcaaacat catcgacact ctcttccttt
     3421 cacccttact cttcccgtcg aagccttatc atgcccttct gaaagacgac aagctgcaaa
     3481 cggattacgc caacaaccct ctgaatgatt ccatcaaggc cagagatctg ttctatgacg
     3541 agattaccca tttcaaccaa actgaagaaa acctgaaaca gatctattat ctcctgctga
     3601 aagagagacc ggagttccgt gcattctttc gacttatcgg gtacacatca gacgagacaa
     3661 accctgaaag tcttattcgc agaaaatttg aaaatgaaat atgtgaacag gctgacctta
     3721 caggaatgat tgaaggagaa ccggtcggac ttgcctattc tcttgcgctc atcaactctt
     3781 tcatccttta cccggaaaaa agatctgtga ctccgccatg ggtcctgaag aattacccgg
     3841 aggtggaaca gataatgttc cgcttgcgga acagaccatg cataagaggc tgcagctatt
     3901 gcaacaaggc cctggatatc cacagtgggc tcaaaagagt gttcggcttt gactcctaca
     3961 gaactttcag gggcgaacca ctccaggagc aggctgtgag agctgccatt aacaataagt
     4021 cgcttgtagc agtgtttccc accggcggcg gaaagtcgct tgccttccag gtgcctgccc
     4081 tcatgagtgg tgataatgta cagggactga cagttgttat ttcgcccctg caatcattga
     4141 tgaaagatca ggttgacaat ctggaaaagg ccgggataac acgcgcagtt acgataaacg
     4201 gcttgcttga ccctgttgaa agggctaaat cgatcgagag ggttgaggat gggtcagcct
     4261 ctcttctcta tatatcaccg gaatccttgc gctcgataac gatcgaacga ctgctgctgg
     4321 gaagaaagat tgtccgcttc gttgttgacg aagcacactg cctctcaaca tggggacagg
     4381 acttcagggt tgattatctg tacattgcgg aatttataaa gtcagtccaa aagaagaaaa
     4441 acctgcagga agctattcct gtctcatgct tcacggcaac ggcaaagcct caggtgatag
     4501 atgatatctg caattacttt atgggtgagc tctcgcttga cctcgagcgc ttcatctcac
     4561 caccttcaag gacaaacctg cagtacaaag tatttgaaaa aggaagcgaa gaagaaaagt
     4621 atcaggcatt aagagatctg attgaagaga ggcactgccc ggcaatcgta tatgtatcaa
     4681 ggacacgaaa ggcataccag ctggcggagc gactgaccaa agatggcttg aatgccaagc
     4741 cataccacgg ccgtatggat gtgcaggaaa aaacggcaaa ccaggaagct ttcatctcag
     4801 gagatgttcc aatcatggtt gccacctccg cattcggaat gggggtagat aaaaaggatg
     4861 ttggcatggt ggtgcactat gagatatccg attctctcga aaactatatc caggaagcag
     4921 gcagggcagg tcgtgatgaa aacatagttg ctgattgctt tgtcctctat aacgaagagg
     4981 accttaataa acactttatc ctcctgaatc agacaaagct gaccatcaag gagatacagc
     5041 aggtctggaa agcaattaag gatatcaccc ggtttcggtc gtcggtatcc aactctgccc
     5101 tggagattgc gcggaaggca ggatgggacg atcgtgtttc tgagatagag acacgggtca
     5161 aaacagccat agcagcgctt gagaatgccg gctatctgaa acggggacag aacatgcccc
     5221 gtgtctttgc caacagtatt ctttcccgga ctgcgcagga agctattgaa aaaatctatg
     5281 cgtcgacaag gtttaatgag aggcagaagc agcaggcagt cagaattatc aggagactct
     5341 tttccagtaa aagcagaaag catgctaccg gcgaagaggc tgaatcgagg gtcgattaca
     5401 tcagtgatca tctcgggata gtaaaggagg aggtgattga gattatcaac ttacttcgcg
     5461 aagagaagat actggctgat gcaaaggatc tgacagcatt tatcaagagc agggaaaaaa
     5521 gaaaccggtc acttaacatc gtcgatacat tccgacagat tgagaactgt ctggtccgga
     5581 tttttgatga agaagcgaag atgtttgacc tcaaagagtt aaatgagaca gcggaagctc
     5641 agggttgcga aggatcaact gtcagcagga tcaaaacggt aatcaacttc tgggcaatca
     5701 aaaactggat taagcgcaag aacctggaat attcgaaaaa ccatgtttca attgtcagcc
     5761 tctatccgcg ggaattactg attgaacggt taaaaaagcg acatgagctg gcaaggttca
     5821 tcattgagtt tctgtgggaa aggtgcaaag ccgatctgtc tgaagaggat gtcgcgaaag
     5881 aggaggtgct ggttgagttt tcggtccttg aactgaaaac agaatatgag agtcagcaga
     5941 gtcttttccg gactgaggtg tcacctgatg acattgaaga gactcttttc tggctttcaa
     6001 ggattgatgc aatcgatatt gaaggtggct ttttggttac atacaacaga cttactattg
     6061 aaaggcttga gcaggacaac agaaagagat atacggctga agattatcag aatctgaaca
     6121 ggttttacga caacaaagcc cagcagattc atatcgtagg agagtatgcc aggaagatgc
     6181 tcgttgatta cagggatgcc ctgcagttcg tagaagacta tttccggttg acctatcctt
     6241 cgttcctgaa caagtatttc aggagcagac agagggagat cacccgcacc ctgacacctg
     6301 acaagtttcg ccagttgttc ggagagctct ctcctgccca gctgaaaatc attaatgacg
     6361 ctgaaacaca gcatattgtt gttgcagcag ggccaggcag tggtaagacc agagtgctgg
     6421 ttcacaagct ggcctccctc cttcttgtgg aagatgtcaa gcatgaacag ttgctgatgc
     6481 taaccttctc aagggcggca gccacggaat tcaaaaggcg gctgattggc ctcgttggaa
     6541 atgcagccaa ctttattgag ataaagactt ttcactcata ctgttttgat cttctgggta
     6601 aaattggcac ccttgagaag tctgatgcta ttcttaagac aaccgttgag aagataaaag
     6661 gcagggaggt agaggcgagc cgcatcacaa agacagtcct ggtaattgac gaggcgcagg
     6721 atatgtccga ggaggagttt gcactgatag cagccctgat tgaacgtaat gaagatatga
     6781 gggtgatcgc cgtcggggat gacgaccaga acatctttga attcaggggt tccagttcaa
     6841 gatatcttga gcattttata agggcaaatc aggcatttaa acatgagctg gttgagaact
     6901 tcaggagtaa aaacaacctg gttgaattca ccaatcagtt cgtaatacgt ataaggcaca
     6961 ggttaaaaaa cactccaatt attgccagac aaaccgacaa cggaaaaata aaagtggttc
     7021 gttatctaaa cggaactctt atcgttccat ttgtacatga tctgctcaat accgaactgt
     7081 caggcaccac ctgcgctctc accagaacca atgatgaggc attgcaggta gcaggcttgc
     7141 tcctgagaaa cggaatgccg gccagattga tccaaagcaa tgacgggttc aatctcatca
     7201 atctcgcgga aataagattc tttttgagtc agccgggcct ggccggaaac ataattaccg
     7261 ttactgatga agcctgggat aaagcaaagc gctcattggc acagagatac cttcgcagca
     7321 caaaatatga attgtgcaaa aacctcatcc gggcgttcga ggctgccaat ccaaagatga
     7381 ggtacaggtc agatctggag gtcttcatcc gcgaatcgaa actggaagat ttctacggtg
     7441 agaatgggga gacaattatc gtttccacga ttcacaaggc aaagggcaag gaatttgaca
     7501 atgtcttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac aaagagcaat
     7621 cctcacaacc tgcaactggt tgaaaactcg ctttaagtgt ttaccctttg taagaaaaag
     7681 gttaaattct ggcgcagaac tttcactatt tttgtattac attgtaaaag gggttgtttg
     7741 ttgtaagtaa ttaaagtatg actgattaat ttgtaaacga agaacagatg ccctttctat
     7801 gaataatgca ggttaatatg gaattcattt caatcagatc tctttttact gcttttaatt
     7861 atccgtctaa catcttaaat agaattatat gagcgaaaag tattctgtca gccccagcgg
     7921 cgaaaaattt ccccttccga aaccggagga ttataaagct gagtttgaga aaatcaaaag
     7981 acgcgttaaa caggaacgtg ccaaaggccg cgagatagtt gtggtcatgg gcgttggatt
     8041 cgtcggggct gttatggcag ccattattgc tgataccgtt gacaagaaag gagagccctc
     8101 gaagtttgtg atcggtatgc aacggccaag cgtgcgcagc ttctggaaga tacccctgct
     8161 caatcgcggt atatcacccg ttaaggccga agacccggag gtagacccga tgatcgcacg
     8221 gtgtgtcagg gagaagaaga cactcattgc aacatatacc tatgacgtac tcaagctggc
     8281 agacgtggtg gtggttgatg ttcaatgcga ctacattaag gaggagttcg gcaacgttca
     8341 tgcaggacat actgacatgg cagccctgga agcctcgctg gcgattatcg ccgaacatat
     8401 ccccgccgaa gctctggtgc tcatagagac caccgttgcc cctggcacca ctgagcaggt
     8461 ggcatatccc attatgaaga aagcattcag aaaacgtgga atcactactg atccgctcct
     8521 ggcacacagc tatgaaaggg tcatgccggg acgtaattat gtagccagta tccgtgattt
     8581 ctggcgggta tgcagcggta tcaacgagga ggctaaaaac cgggtcgtta agttcctgac
     8641 cgaagtgctc aacactgaaa aataccctct cactgtgatg gacaggccca tagagtctga
     8701 aacagcaaaa attgtggaga acagttaccg ggccaccatc cttgccttcc tcgacgaatg
     8761 gagcctcttc gcagagcgaa acggtgtcga cctcatcaag gtgataaagg ccataaaaat
     8821 gagacccact cacagcaaca tgatcttccc cggaccgggg ataggcggat actgcctccc
     8881 gaaggacgga ggcctcggtg tctgggcata caagcacata cttggcttcg aggatgacat
     8941 cttcaggata accccgatgg ccatcgatat caatgacacc cgcggactgc atgtggggca
     9001 gctgacacgc gacgctctcc gcaatatggg caaacctatt gctgctgccg atgtgctggt
     9061 gctgggagcc gcttaccgcg aagatgtggg tgatacccgc tacagcggat cagaggtggt
     9121 catccgcaaa ctggcagaga tgggcgccga gatacgggtc catgatccat atgtggaaca
     9181 ctggtgggag tttgaacagc aggataccta cccggctgaa ggacagagca aggcacagtt
     9241 cttccgcaac caggagaaac taaaagagat acgtattgaa cagaacctga ataaggctct
     9301 caaaggcgct gaggcagtga ttctggccgt accccactct gcatacctca accttgatcc
     9361 tgacaaggtg gtgaaaacca tcggacagcc ggcagcaatc atagattgct ttggtatact
     9421 gacagatgat aaaatccgcc gttacttcca gctgggctgc gaggtgaagg gtctcggccg
     9481 tggccatatc cagaggatca aggaggaaac gagaaaagcc caggagattt aaagattcaa
     9541 aaccggataa agcaccaaac caatgaagag gaaagtagac gaactgtatg atgagtaccg
     9601 caatggcaat gtgagccgac gggaattcat gaaaaagctg gcactggtga ccgggggaac
     9661 cgctgctgca gtggtcatcc tgcccggctg caacggagcc actaccaggg cagccgccaa
     9721 aaacgccatg aaagtggtga gcgagtttat aacctatccg ggcgcttccg gcgacatgcg
     9781 tgcatggatc tcgatcccgg aaggaaagga gaaatacccc ggtgtgcttg ttatccacga
     9841 gaacagggga ctcgtaccgc atatccgtga ggtcaatgac cggatggcca tggaaggatt
     9901 cgtggcgctg gctcccgacg ccctctcgcc tctgggcgga acacccgatg atcagaacaa
     9961 gagccgcgag atgatgggcc agcttgaccg cgaagagaca atcaggaatt tcgtggcagc
    10021 agtgaagtac ctcaaaaccc acccacgcac tacaggcaaa gtgggctgta caggcttctg
    10081 ctggggcggt gcaatgacca accaggtggc agtcaactca cctgaccttg atgcagctgt
    10141 cccctactac ggatcaacac ccgaaccgga agatgtaccg aagatcaggg caaaggtgat
    10201 ggcccactac gcagacaatg acgaacggat caatgcaggc atcccggcat ttgaagaggc
    10261 actgaaaaag gctggcatag aataccatat cttcatctac gagggaacac agcatgcctt
    10321 caacaacgac accggagagc gttacaataa ggaggctgcc gacctggcct ggaaacggac
    10381 aaccgatttc tttaaggaaa aactgaagag ctaactccct tttctccaga gccttgccac
    10441 ttcatttatg aatggccggc aatggttaaa gaagtatctg tacccatcgc acaggtagtt
    10501 tagccccggt tctccgtccg gggcactgat gaacctgttg cgcaggcact cgccattgca
    10561 catatcaaga acttcacaat caagacaata gcggggcagg gttgtttttt tggcttcgcc
    10621 gaaagccttt tgccgggggt catcaagaag gctttccagt gaccggttca tgatattgcc
    10681 aatcagatgc tccccgtcga cgaaatgatc acatgagtag aagtctccat tcatctccac
    10741 tgcaggcaca gccccgcaga ccggcttgaa gatgcatagc gtgtgctcct gcccgaaagc
    10801 ggtgcgcaac gcctcctcga agatctgtac ctttatcttc ccgatatcat ttgcaatcca
    10861 ttcatcgaaa atttcagcaa gaaacctccc gaaggcagcc gcaccggcac ttctcgcggt
    10921 cacctctgat gaattccggc tctttcgttc caccagagga aggaaagtga caaactcaat
    10981 ccccagatct cttaaaaacc tgtatacctc aaggggagca tgcacgttgt tgttgctgac
    11041 aacacacaac acctcgtgcg gcacacgatg tctcctgagg atttctactc ctctcatcac
    11101 atctgcaaat gtgcccctgc cataagccct gagcctgctg ctgtcatgga accgctccgg
    11161 accgtcaagg ctgactccca cataaaaacc ttcctcgctc aggaatctgc cccattcatc
    11221 atccagcagc gttgcattgg tctggatgcc gttaaccatc ctgcactccg gcggagcata
    11281 ctttttttca agcgcaacgg cacgtctgta gaattcaatg cccgccagcg ccggctcgcc
    11341 gccatgccag gagaaaaaga gttctccgcc gccagctgcc tgaatatgct gccgtatata
    11401 tctctccaga actttatcac tcatcctttg cccgccggat tctcttcccg gatgactgtg
    11461 accggaatag tagcagtaac tgcacctgag gttacacctg gcgccaaccg gcttgacaaa
    11521 aacctggaac tcgcggcttt cagaactcat gacaggaatc tcccataatc cggttattca
    11581 ttttgatatc tgtcatcaag gtactcttcg aggttttgcg gaccggcttt catttttacc
    11641 gattgcttct ttccggccag gaagtttctg agactttccg gaatttccac gttctgccct
    11701 gtgagtggct ccacaatctc cctgaatttg gcaggatggg ctgtagccac gaaaatgccc
    11761 actgtcccgg gatgctggtt caggtaagcc tccagaccaa ggcttgcaac agcactatga
    11821 ggatcaagca gatagcctgt gcgttgatac tcctgaccta tatgttccct gatccggtcg
    11881 tcatcaaacc agtagcctga cacagagtct ttgattgctt catagtcatc atcaaatatc
    11941 ctcctgagcc tgacgaaatt gccgggattg cctacatcca tggcatttgc aatggtggca
    12001 accgattctg ccggagtgta tataccagtc ttaaggtaat caggcaccac cctgttgaca
    12061 ttggtagcag ccaccaggtg gtggatgccg aggcctgatc tccgggccat ggctgctgcc
    12121 gttatgttgc cgaaatttcc gcctgggaca gacactgcca tcccgctgcc tgaacggcct
    12181 atggcataat agtaataaaa gctctgaggt atcagccggg caatgttgat ggaattggca
    12241 gatgtgagtt ttaaatttgt gcggtttgca gaaccggaga gagcactctt taccatcgcc
    12301 tgacaatcgt cgaatgaacc ttccacctca atggcggtga tgttccggcc aagagtggta
    12361 agctggttct cctgcaggtt gctcacctta cccgagggat agagaataat tacgtcaacc
    12421 ccttccacgc caaggaaact tgatgctatt gctccaccgg tatcgcctga ggtggccaca
    12481 agcaccaccc tccgtttatc atcacgcgat gaaaaatatc tgaagcagcc tgccatgaac
    12541 cttgccccga aatccttgaa tgcggcagta ggaccatgaa acagttccag tacctgtttt
    12601 cgttcatcca gccgtctgat gattaccggg aagtcaaaag cctccccgca tatctgccgc
    12661 atcactgatc tcggtatatc atctcccgta tagagtgaac ccacctccat gccgatctcg
    12721 ggaagggtca tcgatgccaa ccgtctgaag aagtcatcac tcaaccgggg gatgcgctca
    12781 ggcatgaaca gccccatatc gggcgagatg cccctttcaa ccgcttccct gaagccggtt
    12841 tttaccagcg gatttttcag attataatat ttcatctatt cctgaactgc ccgattacca
    12901 aacctgcgca cccctcctgc ttacgggtga cacgtaggca tcaaatccaa tcccctgcga
    12961 tttcattgct tccgacatac ctttcatcac cgctgccgcg gaatcgccgc ccctgcacag
    13021 tgcaaatacc gatggccccg atccggagat tccggctccc aaagctccgc attccatggc
    13081 agtgctcctc accaggtcaa atccctcaat aagtactgac cttttaggtt ccgccacaaa
    13141 atcaatgagc gaacgcccta tcagatcata atcctccctg tagagcccgg ctaccagccc
    13201 tgccagattg ccccactgcc tcacagccgt ggtgagaggc acctgtctgt caagcaatcc
    13261 ccttgcaact gaagtcttta tctctgtctc agggtgaata accacacagt acagttccgg
    13321 cggaacatgg agtctgacaa tatcgagcgg ttcgtaactg cgtactagta tgattcctcc
    13381 gagcaacgcc ggtgacacat tgtctgcatg ggctgcgccc gaagcaagcc tttcgccctc
    13441 cattgcaagc ctgaccattt cagacgaagt gaatttctct ccaagcagaa ggtttgcacc
    13501 aaaagctgcc gcagcagcgc tggcgctgct tgaccctatc ccgctgccag gcctgatacc
    13561 cttttcaatg ctgaccctga atccggtgct gctgccggcc atggcagagg ctgccttaag
    13621 gacaacgcca acaacgttct gttcaggatc cctgctgagt ctgccacaac ccctgatatc
    13681 atcaatgaca accgtaccgg tatcagtcag ttcaagatca accacatcaa acggctcatc
    13741 cagggcaagc cccaggacat caaatccgca actgagattt gataccgttg caggtgcctt
    13801 taccctgatc ctctttcctg ccatgttaac tattgactat ggtttatctg caaatatacc
    13861 ccgctaaact caattaaatc ataatatatc ttacttttgg gaggttatga cgaaatgaag
    13921 atgagactta ctctgcagat tatattgctt tctcttttgt ctctgcaggt tgcaggacag
    13981 aatttcacag acagcaacct gcccattgtc atcatcagca ctgacggcgg cgtgaccatc
    14041 ccggacgagc caaaggtcaa ggctacaatg aagattattg acagggggcc gggagagcgc
    14101 aattatgtga ccgatcagga taatcctctc tatctgaatt ataacggcag aataggtatt
    14161 gaaacaagag gttcatcttc acaggaatca cccaagaaga attacggttt caaaacccga
    14221 aaggaagatg acaagaccaa caataatgtc agcctcctgg gcatgccagt ggagaatgac
    14281 tggatactcg gcggaatggt gtttgacacc gccttcatcc gcgactacta ctgccataat
    14341 ctttacggaa aaatgggcaa ttatgcctcc agggcagtct attgcgaggt aatcataaac
    14401 aataactacg cgggg
//
LOCUS       DAOPBK010000055        14342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4262_length_14342_cov_4.140967, whole genome shotgun sequence.
ACCESSION   DAOPBK010000055 DAOPBK010000000
VERSION     DAOPBK010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4262_length_14342_cov_4.140967"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLA17_04710"
     CDS             <1..723
                     /locus_tag="PLA17_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPK84748.1"
                     /translation="SGGVTHASFGGIGLGYYFGINPVVGAAVFGLGSAIGMEYMSARG
                     KMREDSAIGILWALGMAIGIIFIYLTPGYAPNLLSYLFGSILTVTDGDLIALGIISVV
                     LIAYTAVFYRTLLYISFDESYARTFTRHVDLFRYVSIGLTGLAIVLNIRMAGVIMIIA
                     LLTIPANIVMLFTRRYLRIIVLSTVVSFFGISAGFAISWFTNLPTGATIVAVLVLFWL
                     LARGARSLIRSSNIEKLKKTTI"
     gene            720..2114
                     /gene="lpdA"
                     /locus_tag="PLA17_04715"
     CDS             720..2114
                     /gene="lpdA"
                     /locus_tag="PLA17_04715"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPK84749.1"
                     /translation="MNEFDLIIIGSGPGGYVAAVRASQLGMKVALVEKAETGGICLNW
                     GCVPTKALLKSAQVLEYARRAADYGINAGEAPAPDFGAVIARSRGVADMMSKGVQYLL
                     KKYNVPVISGFGRLKGAGAVEVEAADGTKEIYNAKHIILATGARSRELPNLRQDGKRI
                     IGYREAMTLPSLPESMVVVGSGAIGSEFAGFYRTMGTEVTLVEFMPRILPIEDEEVSR
                     TMERSFKRAKMKVMVNSTVEKVDTTGEKCIVTIKTPRGEVTVETDIVLSAVGITPNIE
                     GIGLEEAGVKTEKGRVVVDEFYRTSVSGVYAIGDIIPGPALAHVASAEAIVCVEKIAG
                     KTPDPVDYCNIPGCTYTTPEVASCGMTEEAAKAAGIETKTGKFMFMASGKASAAGARE
                     GFVKLVFNAADDRLIGGHLIGMNVTEMISSLVTARKMGATAHDLKNSIHPHPTMSEGI
                     MEAAAAALGEAIHG"
     gene            2497..3057
                     /locus_tag="PLA17_04720"
     CDS             2497..3057
                     /locus_tag="PLA17_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK84750.1"
                     /translation="MAKDKDIIDGCLSGSRRDQELLYRRYSPKLYAVCLQYTGNTEDA
                     REVLQEGFIKIFENLSRFSHEGSFEGWMRRIIVNTALERYRNRYYLTRVDDIDEVTEL
                     EAEPDTNDFAGLEAFDLLNMIMELPPKYRMVFNLYAIEGYSHKEVGKMLGISEGTSKS
                     NLSRAREILQKKVMMLTGITRRMVNV"
     gene            3050..4360
                     /locus_tag="PLA17_04725"
     CDS             3050..4360
                     /locus_tag="PLA17_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPK84751.1"
                     /translation="MFEREPNIDVVFRNGLKNLEVLPPADVWDNIPPMPVKRSRLRII
                     SGIAAGVAALVSLALLARWYVNNNTAQELLSEVTVTGSNNHDVSFDIAKAEPVIRPAE
                     ARIVNLPFTPTPAESVTPADIQITEDMPSELIASVDLPERDVLPDKPVQLFSDEITII
                     KAGRFEGTAENLKTTLPDLKGSKTEQRFLLGASLSPAMGFSRSGENVRMAELIDYEKS
                     QPAYTTGLSVAYMISDRLSIQSGIGLSSIGQTISDVAVYAGLSDYYSVKSPYLYSVET
                     AAGLILAGNTDLYLADNNARVGTVIPGNMADPSKYMLTRVGSDIRQVFRYLELPVMIR
                     YKLIDRKVGLNVSGGVAYGFLVDNQAYTGKGSDLIHVGHTEGVSNFNLSSQLGLGMEY
                     NISRQVIFNLEPVFRYYVTPLSEFSGSLYKPYSVGFSSGFFFKF"
     gene            4446..5384
                     /locus_tag="PLA17_04730"
     CDS             4446..5384
                     /locus_tag="PLA17_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPK84752.1"
                     /translation="MKNTAGKAAIAIMFLLSAGFIFTLLVSADSNQQEAADYEECGPT
                     AISWTIPENVTFAGEALPLQNFDTRESLDRELNATAYRHGSTLLTIKRSGRYFGEIER
                     ILKEYGIPDDFKYLACAESDLSNVISPAGATGYWQIMEGTGKEAGMIINREIDERYDL
                     DKSTRFACKYFLKAYERYGSWTMAAASYNNGFRGLSEQVDIQKETNYYDLLLNEETAR
                     YIFRIVALKLVIAEPEKYGFNISPDVLYKPIPFREVKIDTAISSFEQFARQFDTNYKI
                     LKFLNPWLRKPFLTNSERREYTIRVPEKGARTQAGL"
     gene            5394..8228
                     /gene="uvrA"
                     /locus_tag="PLA17_04735"
     CDS             5394..8228
                     /gene="uvrA"
                     /locus_tag="PLA17_04735"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPK84753.1"
                     /translation="MPEKKDEIRVYGARVHNLRNINVTLPRNKLVVITGLSGSGKSSL
                     AFDTIYAEGQRRYMETLSAYARQFLGGMERPDVDRITGLSPVISIEQKTTNKNPRSTV
                     GTITEIYDFLRLLYARAADAYSCESGEKMVRYTDEQILDLVAERYDQHRVYILAPLIK
                     GRKGHYKELFEQLRRKGFLYARINGEIREITQGLKLDRYKVHFIELVIDKFQVGHVTE
                     KRLHDAVLLALDQGDGIMALLDAGDNSLRFYSRHLMCPVTGISYNEPAPHTFSFNSPQ
                     GACPKCNGLGEISEVDISRMMPDPSLSIRKGGIEPLGKYRNIWIFWMIEALGEKYDFT
                     VDTPVGEIPEDVLNKVFFGSDEVLKLSNTPPGVASGYFMTFEGVVNYVGNMEASGGRA
                     ANGTGRGKGQYIQYHVCPECNGTRLKKEALWFRIGGKNIGELAAMDLNELRNWLENVT
                     DDMSGRQKKIAAEILKEIRSRLGFLLDVGLDYLALNRGARTLSGGESQRIRLATQIGS
                     KLVNVLYILDEPSIGLHQRDNRRLTEALRNLRDAGNSVIVVEHDRDMILSADHIIDIG
                     PGAGINGGMIVAEGTPDDILKSDSLTAAYLNGTKAIEVPEKRREGNGKYLTVTGASGN
                     NLKKVTMKVPLGTFICITGVSGSGKSSLINQTLQPALAKKLHGALRVPLPYESIEGAE
                     NIDKVIEVDQAPIGRTPRSNPATYTGVFTDIRGLFAMTPDAKVRGFGPGRFSFNIRGG
                     RCEGCEGAGVKTIEMNFLPDVYVTCPDCGGKRYNRETLEVRFKGRSINDVLEMTINDA
                     VEFFKGIPSIYHKIKTMQDVGLGYVRLGQPSTTLSGGESQRVKLATELSRRDTGRTLY
                     ILDEPTTGLHFDDVKVLLDVLERLVDRGNTVMVIEHNMDVIKVADFIVDLGREGGRGG
                     GEIIFSGTPEEIVRLKDNYTGQFLAPVLEH"
     gene            8262..8978
                     /locus_tag="PLA17_04740"
     CDS             8262..8978
                     /locus_tag="PLA17_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPK84754.1"
                     /translation="MDEAKEMDLIKEAFEQKTWNEIHTMDSWRVLKIIAELVEGFEKL
                     ARIGPSVAIFGSARTHFNNKYYKLAEEIAYQVTRKGYGVITGGGPGIMEAANKGAKRG
                     NGKSIGINIDLPFEQKANPYIDRDKLITYDYFFVRKVMFMKYAQGFIVLPGGFGTLDE
                     LFEAITLIQTKKIGRFPIVLVGTKYWAGLFDWVTNVMLNEEHNISPEDMNLYRIVDKA
                     EEAVGYIDQFYSRYLLSPNF"
     gene            complement(9192..9662)
                     /gene="smpB"
                     /locus_tag="PLA17_04745"
     CDS             complement(9192..9662)
                     /gene="smpB"
                     /locus_tag="PLA17_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPK84755.1"
                     /translation="MRNNSSNIVIKNRRASHDYEFIEKLIAGIKLTGTEIKSIREGRA
                     SLTDSYCAFRNGELYVTGMHISEYYWGNINNHDPLAERKLLLTRKELRRWERKVNETG
                     LTIIITKLFINDRGLAKVEVALARGKRQYDKRDALRKRDHAREIDLQVKERGKK"
     gene            complement(9673..10254)
                     /locus_tag="PLA17_04750"
     CDS             complement(9673..10254)
                     /locus_tag="PLA17_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HPK84756.1"
                     /translation="MDFRFLYQRTKDFIINPGKAWDVVHRENRPIRYVRGSYALPLII
                     LVTVSAFLGSGLFINTTLKPMYSVLAAVNTFLFLYLGIYASAFIVREIMKAMDLGNDF
                     VVAFKLVAYSMAPVFLSLTISKLFESLLFINVLGLYGVYIFWIGMEEMIKPPDHKKMP
                     MIIATAVAMIIIFLILQIVLSKLAQTLYFSVFA"
     gene            10333..11259
                     /gene="miaA"
                     /locus_tag="PLA17_04755"
     CDS             10333..11259
                     /gene="miaA"
                     /locus_tag="PLA17_04755"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPK84757.1"
                     /translation="MDKPMHDIVVITGPTATGKTRLAALVADRLGGEVISADSRQVYR
                     GMDIGTGKDLSDYIVDGRRVPVHMVDIVDAGYRYNVYEYQRDFLRVFEDMSARDVFPV
                     VCGGSGMYVDSIVSGYRLVKVPVNAPLRALLAERSLDELTVMLSHYKNLHNKTDVDTV
                     KRAIRAIEIADYYANHPVDETPFPVRNPLVMALFVDRETRRQRISDRLRARLDEGMIE
                     EVQRLLAGGIHPDDLIYYGLEYKYITLYLTNRLTRDEMIQKLEVEIHRFAKRQMTWFR
                     GMERRGITINWIDGNMSDLEKTDHIIARLHHT"
     gene            11290..11919
                     /gene="pnuC"
                     /locus_tag="PLA17_04760"
     CDS             11290..11919
                     /gene="pnuC"
                     /locus_tag="PLA17_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HPK84758.1"
                     /translation="MFNIDTILFEILGYRMSLLELLAVLTGLAAVWYAARANIITWVF
                     AIVNAVLFFMLYYQVNLYSAMVLQIFFFCNAIYGWFNWRRQSSDGDKPVTLLSHKARV
                     FWVAGIIAFALAMGTVMGKIHLWLPDFFPVKATFVYTDAMIAVMSIAASLLCARLKLE
                     NWILWILVNIMSIAMYAMRGIMLVSVQYVIFLAMAIYGFMQWRRKVNLK"
     gene            complement(11951..12307)
                     /locus_tag="PLA17_04765"
     CDS             complement(11951..12307)
                     /locus_tag="PLA17_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPK84759.1"
                     /translation="MKRKIILKTTALVLVCAFILSGCASTTIINSSPSGAKVYIDGEP
                     VGTTPYTHTDTKIVGSSTPVRLVMDGYEDFNTLLKRNEEANVGAIIGGIFFLFPFLWT
                     MDYKASHTYELIPLYR"
     gene            12681..13781
                     /locus_tag="PLA17_04770"
     CDS             12681..13781
                     /locus_tag="PLA17_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HPK84760.1"
                     /translation="MSCSCSSPAPQSSGSDESLIKVGVFDGHGGAQTCIWEAVEAIKI
                     DKGMTVRLVTTSDIAGGCLDSLDAIIVPGGGGSRQYLNLGHENHERIRAFIANGGGAV
                     GICAGAYLFSSTPGYASMALNGAQAIDIEHDNRGHGLAKFTLSDEGKTIFPELASRDT
                     SFVVYYEGPVFVEAQDTITYTSFATMESDVHEQGNAPANMTNGKPFFIGNYFGKGRVF
                     SSIAHPEATPGMRWMIPRMVRWTLDREFVAYGQNAVRPDLFEQEILFTRDRLRQESAC
                     YPTFLYGTPEDKIEALNWLESNVSWEAKRWVQGLLFDSSADVRVRAAEYVANSEFTRY
                     LPDLRNAVRNEKDPAAREKMSASLSFLEGVLR"
     gene            13851..>14342
                     /locus_tag="PLA17_04775"
     CDS             13851..>14342
                     /locus_tag="PLA17_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84761.1"
                     /translation="MNKQFNFFLKNFRLFYDIDDVEIEYGKSEGKPVSISRKCEDSFW
                     LESNDINRDDVVWKEWAGVKIPFLFEKENGAEIITFTDDKAAINYDIVASAFYFLSGW
                     NEYVSSSRDQSGRVTYGGSIINELNIPEIPVVNYYFNILEVAVSHVCKRNIKRNLWGA
                     NYLA"
BASE COUNT         3636 a         3332 c         3911 g         3463 t
ORIGIN      
        1 agtggcggtg ttactcatgc ttctttcggg ggaataggac tgggatatta ttttgggata
       61 aacccggtgg ttggtgcagc agttttcgga cttggatcag caatagggat ggagtacatg
      121 tcagcccggg gaaagatgcg cgaggacagt gccataggga ttctctgggc gctgggcatg
      181 gccattggca tcatatttat ctatcttaca cccggttatg cgccaaacct gctcagttac
      241 cttttcggga gcatcctgac cgtgactgac ggtgacctga ttgctctggg gataatttcg
      301 gttgtattga tagcctatac ggcggttttc taccggaccc tgctctatat ctcctttgac
      361 gagagttatg cccgcacctt cacacgtcat gttgacctct tcaggtacgt ctcgattggg
      421 ttgaccggtc tggcaatagt gttaaacatc aggatggcgg gggtgataat gatcattgca
      481 ctgctcacca taccggctaa tattgtgatg ctctttacgc gcagatacct gcggatcatt
      541 gtgctctcca cggtggtcag cttttttggc atcagcgcgg ggtttgccat ctcgtggttc
      601 accaatctgc cgacaggtgc gacaattgtt gccgtgcttg tattgttctg gctgttggcg
      661 cgcggtgcgc gaagccttat caggagcagt aacatcgaaa aacttaaaaa aacaacgata
      721 tgaatgaatt tgatctgata atcatcggga gcggtcccgg cggatatgtt gcagcagtaa
      781 gggcatcgca gcttggaatg aaggtggctt tggtggagaa ggcggagacg ggcgggatat
      841 gccttaactg gggctgtgta cccaccaagg ctcttctgaa gagtgcgcag gtgcttgaat
      901 atgccaggcg tgctgccgac tacggcatta atgccggaga ggcgccggcg cctgatttcg
      961 gggcagtgat agcccgcagc cggggggtgg ccgatatgat gagcaagggg gtgcagtacc
     1021 tgctaaagaa gtataatgtg cctgtcatca gcgggtttgg ccggctgaag ggtgccggcg
     1081 ccgtggaggt ggaggcagcc gacgggacga aggagatcta taatgcaaaa catatcatcc
     1141 ttgctacggg agcaagatca agagagctgc cgaacctccg gcaggatggc aaaaggatta
     1201 tcggctaccg cgaagcgatg accctgccgt cgctgcctga atcaatggtg gtggtgggct
     1261 cgggggccat aggaagtgag ttcgccggat tctacaggac catgggcacc gaggttacac
     1321 tggtggagtt tatgccacgc atacttccga tagaggacga ggaggtgtca cgaacaatgg
     1381 aacgctcgtt caaaagagcg aagatgaagg tcatggtcaa ctcaaccgtg gaaaaggttg
     1441 acaccacagg agagaagtgt atagtaacaa taaagacgcc caggggcgaa gtgaccgtgg
     1501 agacagacat tgttctctcg gcggttggca tcactcccaa tattgaaggt atcggtctgg
     1561 aggaggccgg agtgaagaca gagaagggca gggtagtggt tgatgagttt taccgcacat
     1621 cggtgagcgg cgtttatgcc ataggagaca ttatccccgg tcctgcactg gcgcatgttg
     1681 cctcggcaga ggccattgtc tgcgtggaga agatagcagg taaaactcct gatccggttg
     1741 actactgcaa tatacccggc tgcacatata ccaccccgga ggtggcgtca tgcggcatga
     1801 cagaggaggc ggcaaaggcg gcaggcatag agacgaagac cggcaagttc atgttcatgg
     1861 catcgggcaa ggccagcgcg gccggcgcac gcgaggggtt tgtcaagctg gtgttcaatg
     1921 ctgccgatga caggctgata ggcggtcatc ttataggaat gaatgtcact gagatgattt
     1981 cgtcactggt gacggcacgc aagatgggag caacggctca tgacctgaag aacagcatcc
     2041 atcctcatcc gacgatgtcg gaagggatca tggaggctgc ggctgcagca ctcggtgagg
     2101 ctatccacgg gtagtcagtt ataagagtat tggccccagt ataggatggg agttaatccg
     2161 ctggtagtca gccatcaggg catggggtat ggcagcgccc ggaagttaat ccactggtag
     2221 tcagccatca gggcatgggg catggcagcg cccggaagtt aatccactgg tagtcaactg
     2281 tcagggcatc agtcaaggaa gcgccccggg cccccgtcca atggcgggca tgcattgtct
     2341 tatccctcag gccaatgcat ggcggttact gcgcaaccaa ctttattagg tataaatacc
     2401 accattaacg caacctttcg actcttttta acatcaatct atatactgag accggaaatt
     2461 aattcttacc tttataaact cgactagtaa ccgggtttgg cgaaggacaa ggatataata
     2521 gacggctgtt tatccggcag caggcgcgac caggagttgc tctaccggag gtattccccg
     2581 aagctgtatg ccgtttgcct tcagtatacg ggcaatacgg aagatgcgag ggaggtgctt
     2641 caggagggct tcattaagat ttttgaaaac ctgtcgcgct tcagccacga aggttctttt
     2701 gaaggatgga tgaggaggat tattgtcaac acggctcttg agaggtaccg caacaggtat
     2761 tatctcaccc gtgttgatga catagacgag gttaccgaac tggaagccga gcctgatacc
     2821 aatgactttg ccggattgga agcgtttgat ctgctgaaca tgatcatgga gctcccaccc
     2881 aagtacagga tggtgttcaa cctctatgcc attgaagggt attctcacaa ggaggtggga
     2941 aagatgctgg gtatctccga aggtaccagc aaatcgaacc tctcacgtgc cagagagatt
     3001 cttcagaaga aggtgatgat gcttacgggt atcacaagga gaatggtaaa tgtttgaaag
     3061 agagccaaat attgatgtag tattccgcaa cggcctgaaa aaccttgagg tgctgccgcc
     3121 tgcagacgtg tgggacaata tcccgccgat gccggtgaaa cgctcgcgcc tgaggatcat
     3181 ttccggcatt gctgccggtg tggccgcact ggtatccctt gctcttctgg ccagatggta
     3241 tgtaaacaac aacactgctc aggagcttct ctcagaggtt accgtgaccg ggagcaataa
     3301 ccatgatgta agctttgata tcgccaaagc ggaaccggtc atcaggcctg ctgaagcaag
     3361 aatagtaaat ctcccgttca ccccaacacc tgcggagagt gtgaccccgg ctgacataca
     3421 gatcacggag gatatgccct cagagctgat tgcatcagtc gatctgccgg agcgggatgt
     3481 tcttcccgat aagccggtac agctcttttc agatgagatc acgattatta aggccggccg
     3541 ctttgaggga accgctgaga acctcaaaac aaccctgcct gacctgaagg gctcaaagac
     3601 agagcagcga ttcctgcttg gcgcatcact ctccccggca atgggcttct cacggtcagg
     3661 agagaatgtc aggatggcag aactgattga ctatgagaag tcgcaacccg cttacacaac
     3721 cggcctgtct gtagcataca tgatttcaga ccgcctctcg atacagtctg gcataggcct
     3781 gtcgtcaata ggtcagacga tctcggatgt ggctgtttat gcaggccttt ctgactacta
     3841 ttcggtcaag agcccttatc tctacagcgt tgaaactgct gccggcctta tcctggcagg
     3901 caatactgat ctttatctcg ctgacaacaa cgcaagggtc gggacggtta tcccgggcaa
     3961 tatggctgac ccgtccaagt acatgctgac ccgggtgggt tcggatatcc ggcaggtctt
     4021 ccgttacctt gagcttccgg ttatgataag gtacaagctg attgaccgca aagtgggact
     4081 taacgtctca ggaggagtgg cttacgggtt tctggttgac aatcaggcat acaccgggaa
     4141 aggatctgac ctgatacatg tcggccacac ggaaggggtg agcaacttca acctcagcag
     4201 tcagctgggc ctcggcatgg aatacaatat ctcgcggcag gtgatattca acctggaacc
     4261 tgtgttcagg tattatgtca ctccgctcag tgaattctca ggttctctct acaaacctta
     4321 ttccgtcggc ttctcctcag gcttcttctt caaattctga cagccccggc tttcacgctt
     4381 tatttgcttc accccaagca atattttggc tatttttgca gtttcaaatt tgagattgtc
     4441 ataatatgaa aaatactgcc ggtaaagccg caattgccat aatgtttctg ctcagcgcag
     4501 gatttatttt taccctcctc gtttcagctg acagcaatca acaggaagca gctgattatg
     4561 aggagtgtgg gccgacggca atctcctgga ccatccctga aaatgttact ttcgccggtg
     4621 aggcattacc tctgcaaaat tttgacaccc gcgagagcct tgaccgtgag cttaatgcta
     4681 ccgcttaccg ccacggatca accctgctga ccatcaagcg ctcaggacgc tatttcgggg
     4741 agatagaaag aatactcaaa gagtacggta ttcctgatga tttcaaatac ctggcatgcg
     4801 ccgaaagcga ccttagcaac gttatctcgc cggcaggcgc caccggttac tggcagatca
     4861 tggaaggcac cggcaaggaa gccggcatga tcattaacag ggagatagat gagaggtatg
     4921 accttgacaa atccacgcgc tttgcctgca aatatttcct gaaggcttat gagcggtacg
     4981 gcagctggac catggcggca gcatcttaca acaacggctt cagaggcctc agcgagcagg
     5041 ttgacatcca gaaggagaca aattactacg atctgcttct gaatgaggag actgcaagat
     5101 atatcttccg cattgttgct ctcaagctgg tcattgccga accggaaaaa tacggtttca
     5161 acatcagccc cgacgtgctt tacaaaccga tacctttcag ggaggtgaag attgacaccg
     5221 ccatttccag ctttgaacag ttcgcacggc agtttgacac caactataag atcctgaaat
     5281 tcctgaatcc ctggcttcgc aagcccttcc tgaccaacag tgagaggcgt gaatacacca
     5341 tcagggtacc ggagaagggt gcaaggacac aggccggctt atagttacct ctcatgccgg
     5401 aaaagaaaga cgagataaga gtgtatggag cgagggtcca taaccttcgc aatatcaatg
     5461 ttacgctgcc gcgaaacaaa ctggtggtta tcaccgggct cagtggcagc gggaagtcgt
     5521 cactcgcttt tgacaccatc tatgccgaag gacagcggcg atacatggag acgctgtcag
     5581 cttacgcccg ccagttcctt ggtgggatgg agaggcctga tgttgaccgg attaccggac
     5641 tcagcccggt aatttcaata gaacagaaga ccaccaacaa gaatccccgc tctacggtgg
     5701 gaaccatcac agagatatac gacttcctgc gtctgctcta tgcgcgtgct gctgatgcat
     5761 actcctgtga gtcaggggag aagatggtca gatacactga tgaacagata cttgatctgg
     5821 tggcagagcg gtatgaccag catagggttt acatccttgc acccctgatc aagggcagaa
     5881 agggccatta taaggagctt tttgagcaac tgaggaggaa agggttcctc tatgcccgca
     5941 tcaacggcga gatcagggag atcacgcagg gactgaagct cgacaggtat aaggtacatt
     6001 tcatagagct ggttatagac aagttccagg tgggtcatgt gactgaaaag aggctgcatg
     6061 atgcggtgtt gctggccctt gaccagggcg acggtatcat ggcgttgctt gacgccggcg
     6121 ataacagcct gagattttac agtcgccacc tgatgtgtcc cgttaccggc atctcataca
     6181 atgagcctgc accgcacacc ttctctttca attcgccgca gggcgcatgc ccgaagtgca
     6241 acggactggg tgagatctca gaggtggata tcagcaggat gatgcctgac ccctcgctca
     6301 gcatccgcaa gggcggaata gaaccgctcg gcaaataccg caacatatgg atcttctgga
     6361 tgattgaggc cctcggcgag aagtacgact ttaccgttga cacccccgtg ggtgagattc
     6421 cggaggatgt tctgaacaag gtatttttcg gctctgacga ggtgctgaag ctgtcaaaca
     6481 ctcctcccgg cgtagcctcc ggctacttca tgacctttga gggtgttgtc aattatgtgg
     6541 gtaacatgga ggcatcgggc ggcagggctg ctaatggcac aggcaggggt aaggggcagt
     6601 atattcagta ccatgtctgt ccggagtgca atggtacgag gctgaagaag gaggccctct
     6661 ggtttcgcat tggcgggaag aatataggtg agcttgccgc catggacctc aacgagctga
     6721 ggaactggct tgagaatgtg actgatgaca tgtcagggcg gcagaagaag attgccgccg
     6781 agatactgaa ggagatacgc tcacgcctgg gcttccttct tgacgtgggg cttgattatc
     6841 tggcgcttaa tcgcggcgcc cgcactctct cgggtggaga aagtcagcgt atcaggctgg
     6901 caacacagat tggctcgaaa ctggtcaacg tgctctatat cctggatgag ccgagcatag
     6961 ggctgcatca gcgcgacaac cgcaggctga cagaggcgct tcgcaacctc cgtgatgcgg
     7021 gcaattcggt tatagtggtg gagcacgacc gtgatatgat cctcagcgct gaccatatca
     7081 ttgacatcgg cccgggtgcc ggcataaacg gcggaatgat cgtcgccgaa ggcacaccgg
     7141 acgatatcct gaagagtgac tctctgacag cggcatacct gaacggaaca aaagccattg
     7201 aggtcccgga aaagaggagg gagggcaacg gtaaatacct cacagtcacc ggggccagcg
     7261 gcaacaacct taaaaaggtg accatgaaag ttcctctggg caccttcatc tgcatcaccg
     7321 gggtctccgg cagcggcaag tcatcactca tcaaccagac acttcagccg gcactggcaa
     7381 agaagctgca cggcgcgctc agggtacccc tgccctatga gtctattgaa ggagctgaga
     7441 acattgacaa ggtgattgag gtagaccagg cgcccatagg acgcaccccc aggtcaaacc
     7501 ctgcaacata taccggtgtt ttcactgata tccggggcct tttcgcgatg actcccgatg
     7561 ctaaagtaag ggggtttggt ccggggcgtt tctccttcaa tatcaggggg ggcaggtgtg
     7621 agggatgtga gggtgccggc gtgaagacca tagagatgaa cttccttcct gacgtctacg
     7681 tgacatgtcc tgactgtggc ggaaaacgct acaacaggga gacactcgag gtgagattca
     7741 agggcaggtc aataaatgat gtcctggaga tgacaatcaa tgatgcagtg gagttcttca
     7801 aggggattcc ttcgatatat cataagataa aaaccatgca ggatgtgggc ctcggttatg
     7861 taaggctcgg gcagccctca acgaccctct ccggtggtga atcgcagcgc gtcaagctgg
     7921 ccacggaact ctcgaggcgc gataccggac gcacgctcta tatccttgat gaacctacaa
     7981 caggacttca ttttgatgat gtgaaggttt tgcttgatgt actcgagagg ctggttgacc
     8041 ggggaaacac ggtgatggtt attgaacaca acatggatgt aatcaaggta gccgacttta
     8101 ttgttgacct tggcagggag ggtggacgcg gcggcgggga gataatcttc tcgggaacgc
     8161 cggaagagat tgtcaggctg aaggataatt acaccggaca gttcctggca cccgtgcttg
     8221 aacattagaa aagcttatat ttactgacaa aatatttatt tatggacgaa gcaaaggaga
     8281 tggacctgat aaaggaggcg tttgaacaaa agacctggaa cgagatccac acgatggatt
     8341 catggagggt tttaaagatc attgccgaac tggttgaggg atttgaaaaa cttgcgagga
     8401 taggtccgag tgtggctatc ttcggctctg ccagaacaca tttcaacaac aagtattaca
     8461 agcttgcaga ggagatcgcc tatcaggtaa cccggaaagg gtacggtgtc atcaccggcg
     8521 gcggcccggg catcatggaa gcggccaaca aaggagccaa acgcggcaac ggcaagtcaa
     8581 taggtatcaa catagacctg ccttttgagc agaaggcaaa cccctatatt gaccgcgaca
     8641 agcttatcac gtacgactat ttcttcgtcc gcaaggtgat gttcatgaag tatgcacagg
     8701 ggttcatagt gctgcctggc ggcttcggca cccttgatga gcttttcgag gctatcacac
     8761 tgatacagac taagaaaatc ggacgtttcc cgatagtgct ggtgggaacg aagtactggg
     8821 cggggctgtt cgactgggtc accaatgtga tgctgaacga agagcataac atctcaccgg
     8881 aggacatgaa tctataccgg atagttgata aagccgagga ggccgtgggc tatattgacc
     8941 agttctactc aaggtacctc ctgagcccga acttctgatt tcctgcaaat tataaggctc
     9001 tcgaatatct cgtcatcacc acccctgagt ccgaacttct gattacctgc aaaatactgc
     9061 ctttcctgct ccgatgccgt taaggcaaaa gatcacagct gcttatggcc gattatggcg
     9121 cgccggaaga agtcagcagc tttcctgaat aatgcctgat aatccgataa aggtgaggca
     9181 gttactatag cttacttctt tccgcgctcc tttacctgga ggtctatctc gcgtgcatga
     9241 tcacgcttcc tgagagcgtc gcgcttatca tactgcctct tccccctcgc cagcgccacc
     9301 tccacttttg ccagcccgcg gtcattgatg aacagtttcg tgataatgat tgtcagtcct
     9361 gtctcattga ccttcctttc ccacctgcgg agctcctttc gcgtcagcag cagcttcctc
     9421 tccgctaacg gatcatgatt gttgatgttt ccccagtaat actcggatat atgcattccg
     9481 gtcacgtaga gttcgccatt gcggaaggca caatatgaat cagtcaggct ggctcttcct
     9541 tcacgtattg atttgatttc ggtaccggta agctttattc ccgcgataag cttttcaatg
     9601 aattcatagt catgtgacgc cctcctgttt ttgatgacaa tattgctgct attgtttctc
     9661 atcaggagga aatcaggcga atactgaaaa gtagagggtc tgggccagct ttgacagaac
     9721 aatctgaaga atgaggaaga ttatgatcat tgccacagcg gtggcaatga tcataggcat
     9781 ctttttatga tcaggaggtt tgatcatctc ctccatcccg atccagaaga tatagacgcc
     9841 ataaaggcca agcacattga taaagagcag ggactcaaac agcttgctga ttgtcagtga
     9901 gaggaatacc ggagccatgg agtaggccac cagcttgaag gccaccacga agtcattccc
     9961 cagatccata gctttcatta tctccctgac aataaaggca gaggcgtaga tgccaaggta
    10021 gagaaagaga aaggtgttga cagctgccaa caccgagtac atcggtttca gggtggtgtt
    10081 gataaacagc cctgagccca ggaaggcgct cacggtgaca aggattatca gcgggagggc
    10141 atagcttccc ctgacataac gtattggcct gttctcacga tgaaccacat cccaggcctt
    10201 gccgggattg atgatgaaat cctttgttct ctgatataga aacctgaagt ccatggcctc
    10261 tttcttttgc gaacggctaa aataatcaaa attgttatac cctgacatct ttatggctat
    10321 ctttaatttc tgatggacaa gccgatgcat gatattgtgg tgatcaccgg tcctactgcc
    10381 acgggcaaga caaggctggc ggcgctggta gctgacaggc tgggagggga ggtgataagt
    10441 gctgactcga ggcaggttta ccgcggaatg gatatcggga cgggcaagga cctgtcggat
    10501 tacattgttg acggccggag ggtgcctgtg cacatggtcg atatcgttga tgcggggtac
    10561 aggtacaatg tatatgagta ccagcgcgat tttctcaggg tttttgaaga tatgtcagcg
    10621 cgtgacgtgt tcccggttgt atgcgggggc tcgggtatgt atgttgacag tattgtgagc
    10681 ggatacaggc tggtgaaggt acctgtgaac gcgcctttgc gggctctgct ggcagagcgg
    10741 tcactggatg agttgaccgt gatgctttca cattacaaga atctgcataa caagacggat
    10801 gttgacacgg taaagagggc gatacgggct attgagatag ccgattacta tgcaaaccac
    10861 ccggttgatg agaccccgtt tcctgtaagg aatcctctgg tgatggccct gtttgtcgac
    10921 cgtgagaccc ggcggcaacg tataagcgac aggctgcgcg cgcgccttga tgaggggatg
    10981 attgaagagg tgcaacggct gctggcaggg ggtattcatc ctgatgacct gatctattac
    11041 gggctggagt acaagtatat aaccctttat ctgacgaaca ggctcacacg tgatgagatg
    11101 attcagaagc tggaggtaga gatacaccgt tttgccaagc gccagatgac ctggttccgc
    11161 ggcatggaac gccggggcat caccattaac tggatagatg gcaatatgag tgacctggag
    11221 aaaactgacc acataattgc caggttgcat catacataat cagatcacaa tcattcagac
    11281 ctcagatcta tgtttaacat tgacaccata ctctttgaaa tcctcggtta ccgtatgagt
    11341 cttctcgagc tgcttgcagt gctcaccgga ctggcagctg tctggtatgc agccagggca
    11401 aacatcatca cctgggtatt tgcaatcgtc aatgccgttc tcttcttcat gctttactac
    11461 caggtgaatc tctattcagc catggtgctg cagatctttt tcttctgcaa tgccatctat
    11521 ggctggttca actggagaag acagagcagc gacggcgaca agcctgtaac gctgctgagt
    11581 cacaaggcac gggttttctg ggtggcgggc attattgcct ttgcattggc catgggcacg
    11641 gtgatgggga agattcatct ctggctgcct gacttcttcc ccgtaaaggc caccttcgtc
    11701 tatactgatg ccatgatagc ggtgatgagt atcgcggcat ccctgctctg tgcgcggttg
    11761 aagctcgaga actggatact ctggatactc gtcaacatca tgagcatcgc gatgtacgca
    11821 atgcgtggca tcatgctcgt ttcagtacag tacgttatct tcctcgcaat ggcaatctat
    11881 ggcttcatgc aatggaggag gaaagtaaat ctgaaataag cacccggaat aataaagaga
    11941 tgaacctctt ctacctgtaa agaggaatca gttcataggt gtggctggct ttgtagtcca
    12001 ttgtccagag gaacggaaag agaaagaata ttccgccaat aatcgcacct acattagcct
    12061 cttcattcct tttaagaagc gtgttgaaat cctcataacc atccattacc agcctgaccg
    12121 gagtagaact gccaacgatt ttcgtgtcag tgtgggtgta tggggtagtc ccgacgggtt
    12181 caccatcaat gtaaaccttt gccccggaag gtgatgagtt gataatggta gttgatgcac
    12241 aacctgacag aatgaaagca cagacaagaa ccagtgcggt agtcttcaaa ataatttttc
    12301 ttttcatcgt aatacaattt tggttttaag ggtatgatga atcccacatg aataaaaatt
    12361 acaccaatat ctgtttgtgt atttgataca tgcaggtttc atagttctaa agaattaagt
    12421 caatgattga aaaacagtaa agatttatgt atttaacaaa attgattgct tgcaataagt
    12481 acaaagcgct gcaaatataa ttcaattaat tattctaaat caagaaaaaa attgctgcgt
    12541 tggttttcat taatatcata tatgcaggaa ggaacttttc atatcttagc ttcctgtagt
    12601 aaaacgtgag agaccacatc agtacaatta aattttgttt tatgagacat ttgggtgtta
    12661 tagctattgt aatcgcaatt atgagctgtt catgcagcag tccggctccg caatcgtcag
    12721 gttcagatga aagcctgatt aaagttggtg tattcgatgg gcatggtggc gctcaaacat
    12781 gtatctggga ggctgttgag gccattaaaa ttgataaggg gatgactgtc aggcttgtca
    12841 caacgtctga tatagccggt ggatgtctcg attccctgga tgccattatt gttcccggtg
    12901 gtggcggcag ccgccagtac ctgaacctgg ggcatgagaa tcatgagcgc atacgtgctt
    12961 ttattgccaa tggcggtggt gccgtgggta tatgtgccgg agcctatctt ttctccagta
    13021 ctccaggcta tgccagtatg gccctgaatg gcgcccaggc aatagatatt gaacatgata
    13081 accgtggtca tgggttggca aagttcaccc tttccgacga gggcaaaact atttttcctg
    13141 agctggcctc gcgtgatacc agctttgttg tttactacga aggacccgtt tttgtcgaag
    13201 cacaggatac tataacatac acctcctttg ccacgatgga gagtgacgtg cacgaacagg
    13261 gcaatgcccc tgcaaacatg accaacggca agccattctt tattggaaac tactttggca
    13321 agggacgggt tttcagttct attgcacatc cggaggctac tcccggtatg cggtggatga
    13381 tcccaagaat ggttcgctgg acccttgaca gggagttcgt tgcgtatggg cagaatgccg
    13441 tgcgtcctga tctgtttgaa caggagattc tgttcaccag ggacaggctc aggcaggaat
    13501 cagcttgtta tcccacgttc ctgtatggca cacctgagga taagattgag gcgcttaact
    13561 ggcttgaatc caatgtctca tgggaggcca agcgctgggt gcagggcctg ctctttgact
    13621 catcggccga tgtaagggta agggcagccg agtatgttgc caattctgag tttacccgct
    13681 acctgcctga tctgaggaat gccgtcagaa atgaaaagga cccggcagcc agggaaaaga
    13741 tgagcgcttc actcagtttc ctggagggag tgctccggta atgatgaata gcaatctttt
    13801 cgaactcatc aacggcctcg ggccggaata aagggattat tacagcattg atgaataaac
    13861 agttcaattt cttcttaaag aacttccggt tattctacga catagacgat gttgaaattg
    13921 aatatggaaa gagtgaaggt aagccagtct cgataagcag gaaatgtgaa gactctttct
    13981 ggttagaaag taatgacata aacagggatg atgttgtctg gaaggaatgg gctggtgtga
    14041 aaattccctt tctttttgaa aaggagaacg gtgctgagat aataactttt acggacgata
    14101 aggcagcaat caactatgat attgtggctt cagcattcta ctttttaagc gggtggaatg
    14161 aatacgtcag ctcttccagg gatcagtccg gaagagtcac atatgggggc agcataatca
    14221 atgaattgaa tatcccggaa attcctgttg taaactacta tttcaatata ttggaagttg
    14281 ctgtttccca tgtttgcaaa aggaacataa aaaggaattt atggggggcc aattatctgg
    14341 ct
//
LOCUS       DAOPBK010000056        14319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4271_length_14319_cov_5.198542, whole genome shotgun sequence.
ACCESSION   DAOPBK010000056 DAOPBK010000000
VERSION     DAOPBK010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14319
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4271_length_14319_cov_5.198542"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLA17_04780"
     CDS             complement(<1..477)
                     /locus_tag="PLA17_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HPK84762.1"
                     /translation="MRGLTNIEGIYFLGIGGIGMSALARYFLSQDYIICGYDRTRSKL
                     TDALTEEGCIITYEDSIDSLPVLFSEPSGNDRVLIVYTPAIPGESILLNFFRNNGYTI
                     YKRAEILGVISGETDAIAVAGTHGKTTVSTMTAHLLSSSPVGCSAFLGGISVNYNTN"
     gene            complement(640..1749)
                     /gene="murG"
                     /locus_tag="PLA17_04785"
     CDS             complement(640..1749)
                     /gene="murG"
                     /locus_tag="PLA17_04785"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPK84763.1"
                     /translation="MGNRNIRIIISGGGTGGHLFPAIAIANALRRIEPDVRILFVGAE
                     GKMEMQKVPDAGYEIVGLPVSGLVRSGSIRNISVAFRLFRSMIRAGQIIKKFDPDVVA
                     GVGGYASGPVLKQAQQRHIPTLIQEQNSYAGVTNKLLARRAVRICVAYEGMEKYFPKE
                     KIMLTGNPVRQVYDNLASLRGEALRYFNLDPEMPVILLLGGSLGAGTLNDSVCGSMDL
                     IETSGKQMIWQTGSHYYDRMRDAAEARGVTRIRVNGFISRMELAYAAADVIISRAGAG
                     TISELALVGKPVILVPSPNVAEDHQTHNAMALVNREAALLVRDADARDKLVETALELV
                     DDSTRRNSLSENIRKMAIRDADEVIAREVLKMVKR"
     gene            complement(1787..2977)
                     /locus_tag="PLA17_04790"
     CDS             complement(1787..2977)
                     /locus_tag="PLA17_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPK84764.1"
                     /translation="MADVTIKRFFKGDRYLWGLIAFFMLLSLLSVYSSSVSVAYASHH
                     GNTFYFLRSQFIMLMLGLLIIVVTHWIPYIRYMQFATIGLVVAILLLVITLFAGVSIN
                     QATRWLEIPVIGLRLQTSDLAKVALVIYLARGLTVYQHELNNFRVVTMKLVVPVGIVC
                     VLIMSENLSTAVMIFMISMIILFIGRIPIKFILAYVGLGVAVVILFASLLLVFKKDNN
                     RVQVWKNRIEARINKENSADANYQANQANIAISTGKIFGKLPGKSTQRNLLPQSNSDF
                     IFAIIVEEYGLFGAVIVIMAYLALMYRGVQIARKCDYAFPALMVLGLTVMIVFQAFLH
                     MMVAVGIAPVTGQTLPMISWGRTSVIVISFALGAMLSVSRVMNARERQERLSEEEQTD
                     KIPA"
     gene            complement(3067..4404)
                     /gene="murD"
                     /locus_tag="PLA17_04795"
     CDS             complement(3067..4404)
                     /gene="murD"
                     /locus_tag="PLA17_04795"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPK84765.1"
                     /translation="MNKRIVILGAGESGTGSAVLAQQKGFDVFVSDSGTVKEKYREIL
                     VSHNISFEEGKHTPELIMNAAEVIKSPGLLEDNPLVVAIREKGIPVISEIEFAGRYTD
                     ALKICVTGSNGKTTTTNLIYHMMKKAGLNVAMTGNVGNSFAMAVAEGGYDYYVIELSS
                     FQLDGMYDFRAEIAILMNITPDHLDRYGHEMQNYVDSKMRVIQNQTAADNFIWCADDP
                     VTAREVAARIINARSLPFSIDRCEGMAACIENENMILNITQKTSLMTIHDLALKGRHN
                     TYNSMAAAIAGKVLKIRKVTIRESLADFQGVEHRLESVIMVAGINFINDSKATNVNST
                     WYALECVKTDVVWIAGGIDKGNDYSELNTVVKEKVKAIVCLGRDNSKIISAFSDLVPT
                     IVETGSMEEAVKSAYYLAHKGDTVLLSPACASFDLFNNYEDRGQQFKMCVKNL"
     gene            complement(4406..4528)
                     /locus_tag="PLA17_04800"
     CDS             complement(4406..4528)
                     /locus_tag="PLA17_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84766.1"
                     /translation="MKRREDEPGWVRVKRRENEPGGVRVKRRENESGEVRVKRR"
     gene            complement(4662..5942)
                     /gene="mraY"
                     /locus_tag="PLA17_04805"
     CDS             complement(4662..5942)
                     /gene="mraY"
                     /locus_tag="PLA17_04805"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPK84767.1"
                     /translation="MLYYLFRYLDTLGFPGAGVFNYISFRSAAAVITSLLIAMLIGKR
                     IIYFLQRKQIGETIRNLDLEGQYRKQGTPSMGGLIIIASILVPVLLFARLDNVYIILM
                     IITTIWVGFIGFMDDYIKIFKKNKEGLPGRFKILGQTGLGLIVALTLYLSDDVVVRIN
                     VTPPAGVTVATADAAYDDPAGTADNQIAELQQHSTAVNVKSTKTTIPFVKNNEFDYAW
                     LVPFARGKTKQLLGWLVFMAMVVFVVVAVSNGVNLTDGLDGLATGTSAVAGVTLGILA
                     YVSGNIIYASYLNIMYLPNIGELVIFAAAFAGALVGFLWYNSYPAQVFMGDTGSLTLG
                     GIIAVFAIVIRKELLIPILCGIFFVESLSVMLQVSWFKRTRRKYGEGRRIFLMAPLHH
                     HYQKKGYAEPKIVTRFWIVAIILAVISVVTLKTR"
     gene            complement(5944..7401)
                     /locus_tag="PLA17_04810"
     CDS             complement(5944..7401)
                     /locus_tag="PLA17_04810"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPK84768.1"
                     /translation="MKELKHILKELSVTQIYGDQAKLITAVTADSREVKAGSLFIAVR
                     GTKCDGHAFISQAVEAGAAAVICEQLPEVLHEGVTMIVVDDSAAATGMAASAFYDHPS
                     RRLRLTGITGTNGKTTIATLLYKMFDVLGYKCGLLSTVCNYVFRTRCEATHTTPDPVR
                     LNALLAAMVDKGCEYVFMEVSSHAVEQKRIAGLHFAGGIFTNLTHDHLDYHGTFDNYL
                     AAKKKFFDGLTPEAFALVNNDDRNGSVMIQNCRASKHTFSMRGMADFRCNIIEQGFGG
                     MLLKINGTEVWTRFIGDYNASNLLAVFGGAILLGADKEEVLPVLSSLRPVDGRMEVIR
                     SDMGVIGIVDYAHTPDAVENVIEAINRLRQGAGRLIVVVGAGGDRDRTKRPVMARIAA
                     AGADRLILTSDNPRSEDPDTIIDEMQAGLAPDAVSKMIRITGRADAIRAAVMLATPGD
                     IVLVAGKGHETYQEIKGVRHHFDDREELRKAFKMS"
     gene            complement(7446..9479)
                     /locus_tag="PLA17_04815"
     CDS             complement(7446..9479)
                     /locus_tag="PLA17_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPK84769.1"
                     /translation="MKKTNKPKDQILARTGVIYGIVAVMAIAVFIRIIILQFVQSDKW
                     AAMADKVVFRRHVDIAARGDILSDDGRILASSVPYYTVYMDTRSSGMADTTWANGING
                     LCRGLSKHVGGNTPEGWKNALTSARKRGDRYYLVRRHVSYETLKHLKELPIFREGRFR
                     GGLLYQPENRRIMPNGLLARRTIGYITEDTLANIVGIEGSFNSYLAGKDGLKVQQRLT
                     GGAWIDVDNLGTIHARPGNDIVTTIDLDLQDATENALYKQLVKHKAHHGCAVVMEVKS
                     GDIKAIANLETGSDGLYHETYNYAVGESTEPGSTFKLMSMIVALEDGVIDLHDTINTG
                     DGTVKYYDKVIRDHEDKGLGKITAREVFEHSSNVGTAKIIYDNYKDNPRRYVDRLCSM
                     MINKPLGLQIKGEGKPVIRAPGDKLWSGVSLPMMSYGYEVQMTPLQILTFYNAVANGG
                     RMVKPRFVTEVRDGNRVVKSFGTEVITNAICSRSTIRKAQSLLEGVVEDGTAKNLKNS
                     NYKIAGKTGTAQIANARHGYRSGSGISYQASFVGYFPADNPLYSCIVVVNSPSNRVYY
                     GNVVSGPVFKEIADKVYANSFYRDLRALNPEGDEKVSLREAASEVGKNDLTVAGGVMP
                     NVVGMGLRDAVYMLENSGLRVHYTGRGRVVKQSPQPGVRVSRGATVAVELRLR"
     gene            complement(9525..>10041)
                     /locus_tag="PLA17_04820"
     CDS             complement(9525..>10041)
                     /locus_tag="PLA17_04820"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HPK84770.1"
                     /translation="EDQAPGQRRSKMSGQTEDQAQGQMRNHVRDQVHSHAQVQTSRYS
                     EGQGTQAKSSPAEGKGKGNGKSRGIFLKGLISGTVISDSIILKDIRYSVLIVLLAIIF
                     IANRFNAERMEREITVLEQEVRDLRAEALSVSADLGSVSRQSEITDLVKERGLGLEEL
                     REPPYRIVVNE"
     assembly_gap    10042..10141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10142..10321)
                     /locus_tag="PLA17_04825"
     CDS             complement(<10142..10321)
                     /locus_tag="PLA17_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84771.1"
                     /translation="MRRMGEPGDRYQNDMIYRAMEDDRRSSGYNPADHEPGNQGLEED
                     QAPGQRRSKMSGQTED"
     gene            complement(10318..11217)
                     /gene="rsmH"
                     /locus_tag="PLA17_04830"
     CDS             complement(10318..11217)
                     /gene="rsmH"
                     /locus_tag="PLA17_04830"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPK84772.1"
                     /translation="MTVYHLPALLEESIAGLNLKPEGIYVDVTFGGGGHSRAILEKLT
                     TGRLIAFDQDSDAVANFINDERFTFLCQNFRYLKNNLRYHGVKAIDGLIADLGVSFHQ
                     FDEQERGFSFRGNAKLDMRMNRQSSVKASDILWSYEEERLADIFYNYGELTTSRRLAA
                     AIVKARAVKPVTTVTDLTVTLRDLVPPREENKFWARLFQSLRIEVNSELVALKEMLTQ
                     ALQLLKPGGRLAVITYHSLEDRLVKNFFRSGNFEGESEKDFYGNVSAPLRVVNRKVIV
                     PTEQEIMNNSRARSARLRIAEKV"
     gene            complement(11217..11681)
                     /locus_tag="PLA17_04835"
     CDS             complement(11217..11681)
                     /locus_tag="PLA17_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPK84773.1"
                     /translation="MATFIGDYSCRLDAKGRVILPSAFKKQMPPAAEDRFVVRKDIFE
                     GCLVLYSIDDWNRQLEKIRSRINPYNREHNIFLRNFFKGTAEVALDSSNRLLIPKRLL
                     DLAGIQKDVVLAGQDGRIEMWAEEVYARIDMPSDDFANLAEKLLGTPDAGSS"
     gene            12329..13168
                     /locus_tag="PLA17_04840"
     CDS             12329..13168
                     /locus_tag="PLA17_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPK84774.1"
                     /translation="MEHSVNPLQVDIEKILADKNPKIHRILPGFVIRYLKRIAHQDSI
                     NEILRKFAHLRGAAFNDAALEYMGITYRAHGVENLPKGGRNIFVSNHPLGGLDGMVFM
                     SELTRHFPEIKFPVNDILLYIENYSDIFLPVNKVGTFGRDAAKLMEEAYASDYQLLNF
                     PAGICSRKIRGVITDLPWQKSFIVKAVQHQRDIVPCYFSGRNSSFFYNLANFRKFIGF
                     KMNIEMLYLVDEMFRQNGKDIDVYFGEPIPWQTFDKFRKPQEWADMVREKTYNLPERF
                     KSS"
     gene            13220..14179
                     /locus_tag="PLA17_04845"
     CDS             13220..14179
                     /locus_tag="PLA17_04845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HPK84775.1"
                     /translation="MENGKAEPLGEAVPAEDLEAELARIGYLRKTNNANNEVYIFDHR
                     NSPLLMQEIGRIRELTFRHAGGGTGKALDLDEYDLDPDRPQKQLIIWDPDNREIIGGY
                     RFIFPEQKDKDFTVNRFASSSYFTFSRKFIKKYLPHTMELGRSFVRPEYQSTAMGRKS
                     LFSLDNLWDGLGAIIIDNPRVRYLFGKVTMYTHYNVQARNMILYFMLKHFPDPDKLAV
                     PDNPIFVDINKEEMERLFVGKHFRDDLKILGHEVRRLGEKIPPLINAYMNLSPTMRCF
                     GTFINHHFGNVEETGIMITVRDIYIEKVNRHLASYREDFDISH"
BASE COUNT         3178 a         4021 c         3379 g         3641 t
ORIGIN      
        1 gttggtattg taatttacgg atatgccacc aaggaaggct gaacaaccaa ccggtgaaga
       61 gcttagcaga tgggcagtca tagtactcac cgtggtcttt ccatgggttc ctgccacggc
      121 aattgcatct gtttcaccgg atattacccc gagaatctcg gcacgcttgt atatggtata
      181 tccgttgttc ctgaagaagt taaggagtat gctctccccg ggtatcgcag gggtgtagac
      241 tatcagcacc cggtcattcc cggaaggctc cgaaaacagc acgggaagag agtcaatgct
      301 gtcttcatag gttatgatgc acccctcctc cgtgagagca tcggtaagct tgctcctggt
      361 acggtcatac ccgcagatga tgtaatcctg tgacagaaaa tagcgcgcca gcgcgctcat
      421 tcctatccct cctatcccca ggaaatagat accctctatg tttgtcagtc ccctcatcag
      481 ctattctgta tttaactatc cgtcactttt cttttgtcct gttttctctc tggtggttcc
      541 ctgttttctc tctggtagtt ccctgttttt ttctctcttc tatcgttttt tccgttcttc
      601 gccctgatta tcacttcttg ttcaccggga ggcgtcatat tatctcttca ccattttcaa
      661 tacttccctg gcaatcacct catctgcatc gcggattgcc atcttcctga tgttctctga
      721 cagtgagttt cgccttgtgc tgtcatcaac aagctccaga gcagtttcaa caagcttatc
      781 ccgggcatcc gcatcgcgca ccagcagtgc tgcctccctg ttcaccagcg ccatcgcgtt
      841 gtgcgtctgg tggtcttccg ccacgtttgg cgatgggacc agtatcaccg gtttgcccac
      901 cagtgccagc tctgagattg taccggcgcc ggcacgcgag attattacgt ctgcggcggc
      961 ataggccagc tccatgcggc tgatgaagcc gttgaccctg atgcgggtta cacccctggc
     1021 ctcagccgca tcgcgcatac ggtcataata atggctgccg gtctgccaga tcatctgctt
     1081 ccccgatgtc tcaataaggt ccatgctgcc acacacactg tcattcagcg tgccggcgcc
     1141 aagtgacccg ccaagaagca gtatcaccgg catctccgga tccaggttaa aatacctgag
     1201 agcctccccg cgcaacgagg cgaggttgtc atatacctgc ctgacaggat tgcccgtaag
     1261 cattatcttc tccttcggga aatacttttc catcccctca taggccacgc agatacgtac
     1321 ggctctcctg gccagcagtt tgtttgtgac acctgcatag ctgttctgct cctgtatcag
     1381 agtcggtata tgacgctgct gtgcctgttt caggaccggt cctgatgcat atccacccac
     1441 tcctgcaacg acatcaggat caaacttttt gattatctgc cctgccctga tcatgctgcg
     1501 gaagagcctg aaagcaacgc tgatattcct gatgctgcca cttctgacca gtccggacac
     1561 cggcagcccc acaatctcat aaccggcatc gggtaccttc tgcatctcca tctttccctc
     1621 ggcgcccaca aacaggatcc tcacatccgg ctctatcctc cgcagagcat tggcaatggc
     1681 aatagccggg aagagatgac cgccggtgcc gcccccgctg atgatgatcc ttatgtttct
     1741 gttgcccatg gttatctgta attcatctgt ttcttccctt tgccggtcat gccgggattt
     1801 tatctgtctg ttcctcctca gatagtctct cctgccgctc ccgcgcattc attaccctcg
     1861 atacgctcag catcgcaccc agtgcaaagc tgatcactat caccgaggtg cgtccccagc
     1921 ttatcatcgg cagcgtctgc cccgtcaccg gtgcgatccc cacagccacc atcatgtgca
     1981 ggaaagcctg gaatactatc attaccgtca gccccagcac catcagggcc gggaacgcat
     2041 aatcacattt tcgcgctatc tgcacccccc ggtacatcag cgccagataa gccattatca
     2101 ctattaccgc tccgaagagc ccgtactcct ctacaatgat cgcaaagatg aagtcggagt
     2161 tcgactgcgg aaggaggttt ctctgggtgc tcttgccggg cagcttgccg aagattttgc
     2221 ccgtggagat ggctatattg gcctggtttg cctgatagtt ggcatcggca gagttctctt
     2281 tgttgatacg tgcctcaata cggttcttcc atacctgcac acggttgtta tccttcttga
     2341 agacaagcag cagcgaggca aagagtatca ccactgccac ccccagaccc acataggcca
     2401 ggataaactt aatgggaata cggccgataa aaagtatgat catgctgatc atgaagatca
     2461 tgacagcggt ggagaggttc tccgacatga tcagtacaca gactatcccg accggcacca
     2521 ccagcttcat ggtcaccact ctgaaattgt tcagctcatg ctgatataca gtcagtcccc
     2581 gcgccaggta aatcacaagc gccaccttcg caaggtcaga ggtctggagt ctcaacccga
     2641 tcacaggtat ctcaagccag cgggttgcct ggttgatgct cacccctgcg aaaagcgtaa
     2701 tcacaagaag cagtatcgcc acaaccagcc ctatggtggc aaactgcata tatctgatat
     2761 aaggtatcca gtgggtgaca acaatgatca gcagacccag catcagcatg atgaactggg
     2821 agcggagaaa atagaaggtg tttccgtgat gcgacgcata ggccacgctg accgacgagc
     2881 tgtagaccga cagcagcgac agcagcatga agaaggcgat gaggccccac agatacctgt
     2941 cgcccttaaa aaaccttttt atcgtcacat ccgccatact atcatttttg cttcatcagt
     3001 aatcccttct cattctcctc ccgggtgttc ctcccttttt accctctcta agcatttccg
     3061 ccacccctac agattcttca cacacatttt aaactgctgc ccgcgatcct cataattatt
     3121 gaacaggtcg aagctggcac acgccggcga cagcagcacc gtatcgccct tgtgagcgag
     3181 ataataagct gacttgaccg cctcctccat cgagcctgtc tcgacgatgg tggggaccaa
     3241 atcgctaaag gcactgatga tcttcgagtt atccctgccg aggcaaacga tggccttcac
     3301 cttctccttt accacggtgt tgagctcgct gtagtcattc cccttgtcaa tgcctccggc
     3361 gatccacacc acatcggttt tcacgcattc aagggcatac caggtggagt tgacattggt
     3421 cgccttcgaa tcattgatga aattgatgcc tgccaccatg atgactgact ccagccggtg
     3481 ctctactccc tggaagtctg ccagactctc gcgtattgtc accttgcgga ttttcagaac
     3541 ctttccggcg atagccgcgg ccatcgagtt gtaggtattg tggcgtcctt tcagcgccag
     3601 gtcgtgaatg gtcataaggc tggttttttg ggttatgttt agaatcatgt tttcgttttc
     3661 aatgcatgct gccatcccct cgcacctgtc aatggagaat ggaagggaac gggcgttgat
     3721 gatgcgcgcg gccacctcac gggcagtcac cggatcatct gcacaccaga tgaaattgtc
     3781 agctgcggtc tggttctgaa tcactctcat cttggagtcg acgtagttct gcatctcatg
     3841 gccatacctg tcgagatggt caggggtgat gttcatcaga atggcgattt ctgccctgaa
     3901 gtcatacatt ccatcaagct ggaagctgct gagttctatc acgtagtaat catatccgcc
     3961 ctctgccacc gccatcgcga agctgtttcc cacgttcccg gtcatggcca cgttgagccc
     4021 ggccttcttc atcatgtgat agatcaggtt ggtggttgtg gtcttcccgt tgcttccggt
     4081 cacacatatc ttgagagcat cagtataacg gccggcaaat tctatctcgg aaatcaccgg
     4141 tatgcccttc tcccggatgg ccacaacaag cgggttatcc tcgagcaggc ccgggctctt
     4201 gatcacctcc gcagcgttca taatcagctc aggggtgtgc ttcccctctt caaaagagat
     4261 gttgtgagag accagtatct cacggtactt ttcctttacc gtgccactat cactcacgaa
     4321 aacatcaaat cccttctgct gagccagcac ggctgatccc gtaccgctct ctcccgcacc
     4381 cagtattaca attcttttgt tcattctatc ttctcttcac ccgtacctcg cctgattcat
     4441 tctctcttct cttcacccgc accccgcctg gttcattctc tcttctcttc acccgcaccc
     4501 aacctggttc atcctctctt ctcttcactc acaccccgcc tggttcattc tctcttctct
     4561 tcactcacac cccgcctggt tcattctctc ttctcttcac ccgcaccccg cctggttcat
     4621 tctctgccgt atctgcaatg ccaccgggcc tggttcgctt tctatcttgt tttcagtgtc
     4681 accacgctga tcacagccag tattattgcc acaatccaga aacgtgtaac tatctttggt
     4741 tcagcatatc ccttcttctg atagtggtga tgcagcggcg ccatcaggaa aatgcgtctc
     4801 ccctcgccat atttccgtct ggttctttta aaccagctta cctgcagcat caccgagagg
     4861 ctctccacga agaagattcc gcagaggatt ggtatcagaa gctcttttct tatcacgatg
     4921 gcgaagacgg caatgatccc tccgagggtc aggctgccgg tatcgcccat aaatacctgt
     4981 gccgggtagg agttatacca caagaacccc acaagggctc ccgcgaaggc ggcggcaaag
     5041 atcaccagct cgccgatatt cggcaggtac attatgttaa ggtagctggc atagatgatg
     5101 ttacccgata catatgccag aatcccgagc gtcactcccg ccacggcgct ggtacccgtc
     5161 gccagtccgt cgaggccatc agtcaggtta accccgttcg aaacggcaac aaccacaaat
     5221 accaccatag ccataaagac cagccagcca agcagttgtt ttgttttgcc cctggcgaag
     5281 ggaacaagcc acgcatagtc aaactcattg ttcttcacga aggggatggt tgtcttggtc
     5341 gatttgacat ttacggctgt agagtgttgt tggagttcag cgatttgatt atcagcagta
     5401 ccggccggat catcatacgc ggcatcagcc gtagccacgg tgactcctgc cggcggagtg
     5461 acatttatgc gcacaacaac atcgtcgctc agatagaggg tcagggctac tatcagacca
     5521 agccctgtct gtccgagtat cttgaacctg ccggggagcc cctccttgtt ctttttgaat
     5581 atcttgatgt aatcatccat gaacccgatg aatcccaccc agattgtggt tatgatcatc
     5641 aggatgatat agacgttgtc gagcctggcg aaaagaagca ccggcacgag gatggatgct
     5701 attatgataa ggccgcccat tgagggggtg ccctgcttgc ggtactgtcc ctcgagatcg
     5761 aggttgcgga ttgtctcccc tatctgtttg cgctgcagga aataaatgat ccttttgccg
     5821 atcagcatgg caatcagcag ggaggtaatg acagctgcgg cagacctgaa tgagatgtag
     5881 ttgaaaaccc ctgcgccggg gaaacccaat gtgtcaagat atctgaagag atagtacagc
     5941 atgtcaactc attttaaatg ctttgcgcag ttcctccctg tcatcgaagt gatgtctcac
     6001 acccttaatc tcctggtagg tctcatgccc cttgcctgca accagcacga tgtcacccgg
     6061 cgtggccagc atcactgcag cccttatggc atcggcgcgg ccggtgatgc gtatcatctt
     6121 agagacagca tccggtgcaa ggccggcctg catctcatca ataatggtgt cagggtcctc
     6181 gctgcgtgga ttgtcggagg tcagtatcag cctgtcagca cccgcagcgg caatacgtgc
     6241 catcaccggc cgcttcgtcc ggtcacggtc gcctcccgct cctaccacta ctatcagcct
     6301 gcctgcaccc tgacgcagcc tgttaatggc ttcaatcacg ttctctaccg cgtcaggggt
     6361 atgtgcatag tcaactatgc cgattactcc catgtcagag cggataacct ccatgcggcc
     6421 gtcaacagga cgaaggctgc tgagtaccgg cagcacctcc tctttatctg ctccaagcag
     6481 aatggctccg ccaaaaacgg ccagcaggtt tgatgcgtta tagtcgccta tgaaacgggt
     6541 ccatacctcg gtaccgttga tcttcaggag cattcccccg aatccctgtt ctattatgtt
     6601 gcaccggaag tcggccatgc cgcgcatgct gaaggtatgc ttcgaggctc tgcagttctg
     6661 tatcatgacg ctcccgttgc ggtcatcgtt gttgacaagt gcaaacgctt cgggcgtgag
     6721 cccgtcaaag aacttcttct tggctgccag gtaattgtca aaagtcccgt ggtaatcgag
     6781 atggtcatgt gtaaggttgg taaaaatgcc tccggcaaaa tggagcccgg caatgcgctt
     6841 ctgctcaacg gcatgggagc tcacctccat aaatacatac tcacatccct tatcaaccat
     6901 cgcggcaaga agggcattga gcctgaccgg atcaggggtt gtgtgggtgg cttcgcaccg
     6961 tgtgcggaag acatagttgc agaccgtgga gaggaggccg cacttgtaac ccagcacatc
     7021 gaacatttta taaagaagcg tggctatggt ggtcttgccg ttggtgccgg tgatgcccgt
     7081 gagtctcaac ctgcgcgagg gatggtcata gaaggccgag gctgccatgc ctgttgcagc
     7141 agcagagtca tctacaacga tcatggtcac accctcatga aggacttccg gaagctgctc
     7201 acagataaca gcggcagcgc ctgcctccac ggcttgactg atgaatgcat gaccatcgca
     7261 cttcgtgcca cgaacagcta taaaaaggct tcccgccttt acctcacgcg agtcagccgt
     7321 aacagcggtg ataagctttg cctgatctcc gtagatctgt gttacactca gctccttcag
     7381 tatatgtttt aactccttca tcacttcatt ttcatctcat ctgcacctgt atccctgact
     7441 gacgctcatc tcaacctcag ttctactgcc accgttgctc ccctggacac cctgactccg
     7501 ggctgtggcg actgcttcac cacccttccg cgaccggtgt aatgcacccg cagcccgctg
     7561 ttctcaagca tgtaaaccgc atcgcgcaaa cccatcccca ccacattcgg catgaccccc
     7621 ccggcaaccg tgaggtcatt ctttcccact tcacttgcag cttcgcgaag cgaaaccttt
     7681 tcatcaccct ccgggttgag tgcacggaga tcacgataaa agctgtttgc ataaaccttg
     7741 tcagcaatct ccttgaatac gggacccgat accacgttcc cgtaatagac ccggttggaa
     7801 ggtgaattga caaccacaat acaggagtag agcggattat cagcggggaa gtatcccaca
     7861 aacgatgcct ggtatgatat ccctgagcct gagcggtacc cgtgtctggc gtttgctatc
     7921 tgcgccgtgc cggttttgcc cgcaattttg taattgctgt tcttcaggtt tttggcggtg
     7981 ccatcttcaa cgacaccttc gaggagcgac tgcgccttgc ggatggtgga ccgggagcag
     8041 atggcattgg tgatcacctc ggtgccaaac gatttcacta cccggttgcc atccctgacc
     8101 tcagtgacaa acctgggctt taccatcctg ccgccgttgg ccacggcatt atagaaagta
     8161 agtatctgca ggggagtcat ctgcacctcg taaccgtatg acatcattgg cagcgagaca
     8221 cctgaccaga gcttgtcgcc cggagcacgg atgacgggct taccctcacc cttgatctgc
     8281 aggccgagag gcttgttgat catcatcgaa cagagacggt caacgtaccg gcgggggttg
     8341 tccttgtagt tatcgtagat gattttggcg gttcccacgt ttgaggagtg ctcaaacacc
     8401 tcgcgggcag tgattttgcc cagcccctta tcctcatgat cacgtatcac cttgtcataa
     8461 tactttacag tgccgtcgcc ggtgttgatt gtatcatgaa gatcgatgac tccatcttcc
     8521 agtgcaacga tcatcgacat cagcttgaag gttgagccgg gctccgtgct ttcgccgacg
     8581 gcgtagttat aggtctcatg atataatccg tcagagcctg tctcaaggtt tgcgatggcc
     8641 ttgatgtcgc cgctcttcac ctccatcacc acggcgcaac cgtgatgggc cttgtgtttc
     8701 acgagctgct tgtacaaagc gttctctgtc gcatcctgaa ggtcaagatc gatggtggtg
     8761 acaatgtcat tcccggggcg ggcgtgtatc gtcccgaggt tatcaacatc aatccatgct
     8821 cctcctgtca ggcgctgctg caccttcagc ccgtccttcc ccgcgagata actgttgaat
     8881 gacccctcaa tgccgacgat gttcgcgagc gtgtcctccg tgatatatcc tattgttcgc
     8941 ctcgcgagaa gtccgtttgg cataatgcgc ctgttttcag gctggtagag cagaccgccg
     9001 cggaatctgc cttcacggaa gatcgggagc tccttcagat gcttgagtgt ctcgtaactc
     9061 acatgtctcc tgacaaggta atacctgtct ccacgcttgc gtgcactggt gagggcattc
     9121 ttccacccct cgggtgtgtt gccgccgaca tgcttcgaga gaccgcggca gagtccgttg
     9181 atgccgttgg cccaggtggt gtcagccatg cctgagctgc gggtgtccat gtagacagtg
     9241 tagtacggga ctgagctggc caggatgcgg ccgtcgtcag agaggatatc acccctggca
     9301 gctatgtcaa catgcctgcg gaacacaacc ttgtcagcca tggcggccca cttgtctgac
     9361 tgcacaaact gcaggatgat gatacggatg aagacggcga tagccatcac tgccacaata
     9421 ccgtaaataa ccccggtacg ggcaagaatc tggtctttcg gtttgttcgt cttcttcatg
     9481 ggtcaggtat ggttctgtct gtcgggcggt tgaagtttta ccggttattc attcactact
     9541 attctgtatg gcggctcacg gagctcctca aggccgaggc cccgttcctt caccagatca
     9601 gttatctcac tctggcggct cacgctccca aggtcagcag agacggagag cgcctctgca
     9661 cgaaggtccc ttacctcctg ttcgagcacg gttatctcgc gctccatgcg ttccgcgttg
     9721 aacctgttgg caatgaagat tattgccagc aacactatca gtacagagta tcttatatcc
     9781 ttgaggatta tgctgtcaga gatgactgtt ccgcttatca gccccttgag gaagatgccc
     9841 ctgcttttgc cattgccttt acctttgccc tcagcgggcg aagatttcgc ttgagtgccc
     9901 tgcccctcag agtaccttga agtctggact tgtgcatgac tatgcacctg atcccgaaca
     9961 tggtttcgca tctgaccctg tgcctgatct tccgtctgac ctgacatctt gcttcgcctc
    10021 tggcctggtg cctgatcttc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 natcttccgt ctgacctgac atcttgcttc gcctctggcc tggtgcctga tcttcctcca
    10201 gaccctgatt gccaggctca tgatcagcag gattataccc tgatgagcga cggtcatctt
    10261 ccatcgcccg gtaaatcata tcattctggt atctgtctcc cggctctccc atccttctca
    10321 aaccttctcc gctatcctga gcctggcgct gcgtgcacgg ctgttattca ttatctcctg
    10381 ctccgtcggg acaatcacct tgcggttcac caccctgaga ggcgcactta cattcccgta
    10441 aaaatccttc tcactctcac cctcgaaatt gccgctgcgg aaaaagtttt tcaccagcct
    10501 gtcctcaagg gagtgatatg tgatcactgc cagccttcct ccgggcttca gtaactgcag
    10561 agcctgtgtc aacatctcct tcaatgcaac gagctcactg ttcacttcta tccggagcga
    10621 ctgaaacagg cgcgcccaga acttgttctc ctctcttggc ggcacgaggt ctctgagtgt
    10681 cactgtgaga tccgtgactg ttgttaccgg ctttacggcc ctggccttta cgattgctgc
    10741 tgccagcctc cgcgacgtgg tcagctcacc gtagttgtag aaaatatcag ccagcctttc
    10801 ctcctcgtaa gaccagagga tgtcggatgc cttcactgac gactgcctgt tcatccgcat
    10861 atcaagtttg gcgttgccgc gaaacgagaa tcccctctcc tgctcgtcga actggtgaaa
    10921 tgacacaccc agatcggcta tcagtccgtc aatggctttt acaccgtggt aacgcaggtt
    10981 gttcttcaga tacctgaagt tctggcaaag gaaggtgaac cgttcgtcat ttatgaagtt
    11041 tgcaacagca tcagaatcct gatcaaaagc aatcagcctg ccggtggtaa gcttctcgag
    11101 gatggcccgt gagtgtccgc cgcctccaaa tgttacgtcg acataaatcc cttcaggctt
    11161 gaggttgagc cctgcaatgc tttcttccag cagtgccggg agatgatata ctgtcattat
    11221 gatgagccgg catcgggagt gccaagcagt ttctccgcca ggttggcgaa gtcgtcggaa
    11281 ggcatgtcga tacgggcata aacctcctca gcccacattt cgatgcgacc gtcctgcccg
    11341 gccagaacaa catctttttg tattccggca aggtcaagga ggcgctttgg gataagcagc
    11401 ctgttgctgc tgtcaagtgc tacttcggcg gtacccttga agaaattgcg gagaaagata
    11461 ttgtgttccc tgttgtatgg gttgatcctg ctgcgaatct tctcaagctg cctgttccag
    11521 tcatcaatcg agtacaatac caggcaccct tcgaagatat cttttctgac cacaaagcga
    11581 tcttcagctg ccggtggcat ctgcttttta aacgctgagg gaaggattac tcttcccttg
    11641 gcgtcaagtc tgcatgaata atcgccgatg aatgtggcca tcacagttgt tgtttcaaat
    11701 ggctaaagta ggaattaatt tcccactttc ctccactttt ctccattttt cttcactatt
    11761 gttgaaaact ttgtttccgg agcggaaatt gtgtgtgatg gcagaaaaat ttgatatttg
    11821 ctatgtgctg taatacaatg ctttatgacc tttttgaaat attatctgcc ttgcgggcaa
    11881 aatatgattt taaataccca ttctgcaatg ctttatccgc tgctgccctc cgccggatgg
    11941 tcatcctgat tattccgggc caaactgatg attattccgg accaaagtga gccacccatg
    12001 ctgatccaaa ctgagccaga ataaccagtc atcccgggta aaatcatggt ccacggtgcc
    12061 acttcgccgc aggcgagaag gtattgaaac cagcggttac ctccattaat aatccgccgc
    12121 aggcgagaag gtattgaagt cagcggttac ccccattaag aattcaccgc aggcgagaag
    12181 gtattgaagt cagtggttac ccccattaag aattcatcga aagcaatcag gtattgaagc
    12241 cagcgcttgc ctcctgtaac aattcgtagc aggcgataca cgattataaa tcctcatgtt
    12301 tatctttgca atcgtttaag gtgacaaaat ggaacactcc gtcaatcctc ttcaagtgga
    12361 tatcgagaaa attctcgctg acaagaaccc gaagattcac aggattctcc ccggctttgt
    12421 gatccgttac ctcaaacgca ttgcgcacca ggacagcatt aatgaaatcc tgcgcaaatt
    12481 cgcgcatctg cgcggtgctg cctttaacga tgcagccctt gaatacatgg gcatcacata
    12541 ccgtgcccac ggagtcgaaa acctgcccaa aggaggcagg aacatttttg tctcaaacca
    12601 cccccttggc ggccttgacg gcatggtctt catgagcgag ctcaccaggc actttcccga
    12661 aataaaattt ccggtaaatg atatcctgct ttatatcgag aattacagtg acatcttcct
    12721 cccggtgaac aaggttggca ccttcggccg cgatgcggct aaactgatgg aggaggctta
    12781 tgcttcagac tatcagcttc ttaactttcc cgcagggata tgttccagga aaatcagggg
    12841 cgtgataact gacctgccat ggcagaaaag cttcatcgtc aaggctgtac agcaccagcg
    12901 cgatatcgta ccgtgctact tctcgggacg caacagctca ttcttctaca acctcgccaa
    12961 cttcagaaaa tttatagggt ttaaaatgaa catagagatg ctctacctgg ttgacgagat
    13021 gttcaggcag aatggcaagg acattgacgt ctacttcggt gaaccgatac cctggcagac
    13081 atttgacaag ttcaggaagc cgcaggagtg ggctgacatg gtacgtgaga aaacatataa
    13141 tttaccggaa agatttaaaa gcagttaact attatttctt acttttagct ttcaattcaa
    13201 tctttttgtg gataagaaaa tggaaaacgg caaagcggag cccctgggtg aggctgtgcc
    13261 cgcagaagat ctggaagcgg aactggccag gattggttac ctgagaaaga cgaacaacgc
    13321 caataacgag gtatatatct ttgaccaccg gaattcgcca ttgcttatgc aagaaatagg
    13381 ccgtatccgc gagctgacct tcaggcatgc cggaggaggt accggtaaag cgctggatct
    13441 cgacgaatat gacctcgatc cggaccgtcc gcagaaacag ctgataatat gggatccgga
    13501 caaccgcgag ataatcggcg ggtaccgctt catcttcccg gaacagaagg ataaggattt
    13561 tacggttaac cgttttgcct cgtcatcgta cttcactttt tcgcgaaaat tcattaaaaa
    13621 atatctcccg cacaccatgg agctgggacg ctcgttcgtc agaccggaat accagagcac
    13681 cgcaatgggc cgcaagagcc tcttctctct tgacaacctg tgggacgggc tgggggctat
    13741 aatcatagat aacccgcggg tacggtacct gttcgggaaa gtgacgatgt acacccatta
    13801 caatgtgcag gcacgaaaca tgatccttta tttcatgctg aaacacttcc ctgatcctga
    13861 taagctggca gtgcctgata accctatctt cgttgatatc aacaaggagg agatggagag
    13921 gctttttgtc ggaaaacatt tcagggatga cctgaagatt cttggccatg aggttcgcag
    13981 gctgggtgaa aaaatccctc cgctgataaa tgcctatatg aatctttcgc caacgatgag
    14041 atgcttcgga acattcatca accaccattt cgggaatgtt gaggagacag ggattatgat
    14101 cacggtgcgg gatatttaca tcgaaaaggt caaccggcac ctggcatcat acagggagga
    14161 ttttgacatc tcgcattagg ttggcaacct cttcttttac aaagatctgc ctgtagatta
    14221 gctttgggtt atcgctaatc ttaccatatc tgtccgagat tagtttcgcg ttgccgcttg
    14281 ttctacttaa ccacctgggt tttgaaagcg atcacatcc
//
LOCUS       DAOPBK010000057        14077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4349_length_14077_cov_4.678648, whole genome shotgun sequence.
ACCESSION   DAOPBK010000057 DAOPBK010000000
VERSION     DAOPBK010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4349_length_14077_cov_4.678648"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLA17_04850"
     CDS             complement(<1..1233)
                     /locus_tag="PLA17_04850"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPK84776.1"
                     /translation="MKKIRYISRLAGIMVVVLAVNACEPQMADKPEIGAAPTADQLNF
                     TITPGSDAFNFVIENTSSVTGIASWDFGNGTKATGNSNIARYPMPGDYTIAMTLVTRG
                     GMATITKTLTQTETDFSIFTDPVYVNLTGGIDNTAGKTWVLDVATRGHLGVGPVDDPT
                     AMSWWAAAPYDKDGTGLMDDEITFKLDGFVVTYDNKGVSYVKAYRANDPALASVYLNP
                     RENKGDWDVDYATPVSGTFNIIKVSEGKYTITLNGEKPIFPCFDVGAKDNTYTIRTIE
                     ENLLELTCLESYEGWNNWHYYLIPKGYVRPTITYTVNATEGVDNEVSCSVTGYTIPEG
                     QKVTDVTWNFGDGSDEVTAGKDEVVKHVFMRKGTYNVTAKLNTSLGVLTGTKQIVLAN
                     NNSAYVPFLLDMMIVYNDL"
     gene            complement(1257..2867)
                     /locus_tag="PLA17_04855"
     CDS             complement(1257..2867)
                     /locus_tag="PLA17_04855"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK84777.1"
                     /translation="MKKSILYSVFFLAVIVAGSSCSRDFLELEPKTGQTEANYYKTES
                     DAFLAMTAVYDAYALQNWQFAATMADIWSDDAFCGGSDASDMSQWQDMEMFKMEPENN
                     SSSDLWSRCYAGVYRANLYLQKQDGIDWKSEGLKERYEAEVKFLRAYFYWDLVRHFGW
                     VPVMTEVLPSVEDYRSLTQSTPSEIFTLIASDLLDAVDVLPNQVTPAEKGRVTKGAVK
                     AMIARIYLYHTGMSAISGLGLTAEQWGNGSTVINKAYVQTALDQVINSGVYSLIPDYA
                     DLFDWDNQNTVESVLEMQYSDKAKSGDWGGWNINGNFASVWISVRNPQGDPTVFPGWS
                     FSVPTWSLADEFEPGDPRKYVTLYDAEAMLTDYNRAFQNTGYFNKKYMARADYQGSGG
                     DPNHNFPRNFMDIRYADVLLMAAEVWLTDNPGKAAGYFNQVRTRALGAAAEKTTITLD
                     DIYHERRVEFGGEGLRKWDLLRRGLDYAGEKINASFNIPAGTPNASDFAPRTFKPDSW
                     GMFPIPASEIRNMNSGVLKQMVPAYIGL"
     gene            complement(2887..5958)
                     /locus_tag="PLA17_04860"
     CDS             complement(2887..5958)
                     /locus_tag="PLA17_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK84778.1"
                     /translation="MKQTILALFFIACSLALNAQGLQIKGVVTSADDGQPVPGVSVLV
                     KGTTTGTMTDMNGYYSLDNVPMNSLLVFSFVGMKAQEVVVTESTTINIVLASDIELID
                     EVVVVGYGTQKKSLVTGAIAKVEGDDLRKATDMRVTQALQGKTAGVVITANSGQPGSQ
                     ISVRIRGAGTYGDAEPLYIIDGLPMSGAGTDFLNASDIESIEVLKDAASAAIYGARGA
                     NGVVLITTKTGRTDTKLTVTYDGYYGFQNPWKKMPLLNSREYIMLINEASVNAGLGPR
                     FSQAQIEGFTADTDWQDEMFYYNAPKQNHSLSFTGGSDRLEYSSSLNYFSQEGIVSKG
                     KSSFEKVGYRLNAAATFGLFKLGGNVNLAGITSKGIATNDRYGLGLNQALNMPPVIPV
                     MFSDGTWGTSEAYGFGLQEITNPVAMASYNNSRTRTYKLIGNLYGEFDFGKVSPALRG
                     LTFRSSFGGEYALVNTDSYTPEYYLDAMHFSNIDKAYKGLDMWSRWNFENVFTYAKTF
                     DVHNLTIIAGTTAFKDMHENLWGTKNDLIFDDFEHSYLDNATDPESANAGGGYSDHTV
                     SSLFARLNYDLKNRYMLTATIRKDGSSRFGSENKYGYFPSASIGWVISREDFMSNLTD
                     IFNVLKLRASWGQNGNENIGNFNYTSIIGNSAIYYFGDGKTQYNGTVPTKLANPMLKW
                     EASEQTNIGLDMTTLGSTLTVTLDYYIKKTKDWQVAAPIPMLVGNGAPTINGGEVKNS
                     GFEAQVSYRKQIGKLFLGASVNGAFNKSEVLDLKNTDKVLKGGDGGFGQSELIYVQVG
                     FPMGVFFGIKTDGVFQNQGEIDAYVNRAGEKIQPNASPGDIKFIDSNGDGTISDGDRV
                     QIGSPLPDFTGGLNLNAEIYGFDFNMFLYAALGQEIYSATRRYDMNYANYTADWLDRW
                     TGPGTSNYYPRVTFTDNNLNHKTVSDFYIQDGSFVRLRNITLGYSLPQNITKAIKLKK
                     LRFYLSAENLYTFTKYTGYDPEIGGGVFDNGIDRGIYPQPRTIMTGINVTF"
     gene            complement(6218..9115)
                     /locus_tag="PLA17_04865"
     CDS             complement(6218..9115)
                     /locus_tag="PLA17_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HPK84779.1"
                     /translation="MIRRLISVVFFLLPAVYNATAEVQEIGTPFIRNFTKADYRASTQ
                     NWAVAQDARGFMYFANNDGLLVYDGVQWHLYRMPNYSMVRSILMGAKGEIFIGAYNDF
                     GKMVPEAGGNLSFQSFRELIPEEYRNFDDVWNIFTYRDKTVFQSYYAAYFYRDGTPMT
                     VVAAPSRFQNSYTVRGRLLFNDTEYGLMEFDGTRLARLPGCESLTGMDICSLLPYGSG
                     NEMMICTLGNGLFIYDGAKLRKWDSALSSILSRDQVFSATMIYDDYYVMGTILNGVMI
                     IDPEGRIVQHINRKNGLQNNTVLALFTDRNGNLWLALDNGIDYVTINSPVTFIRSTEG
                     FGAGYASVIHDGKLYLGTNQGLYVRNWSERETGDDFRMIPGTYGQVWYLGVHNGILLC
                     GHNNGTYLVSGEKADLISRVSGGWKYMELKNHPGYLLGGTFSGMILLRWEQGTWKFVR
                     QIPGMTESFRVFEEDREGEIWMSHGFKGIFRVRLSAELDSVTSVRFYDSSDGLPSDFY
                     LGIFRVLGQLVVVSETEIYEYSPETDRFVRSTYFNQLFSPLVAVSHLHEDKEGNIWYV
                     AKNRAGVFRLKEDHTYEHIATPFLLLSGGFIHGFESFYPYAPEHVIIGTEDGFAHYSS
                     RTSSLGYEGFSVYITLGEALHHDSVFYYGRTPSSAVREISYRFRYANNNMRFMFASPV
                     YDNAGNTEYSYRLEGYDDRWSDWSRTYYKEYSNLPDGKFTFHVKARNQLGIESKPDSL
                     EFVIEHPWYKSPVAIIAYVIAGIIMVLLLARFISWRIDVSRRNERVKNARIYEEKEQE
                     YKRQALESEKEIIRIRNERLQAEMILRDKELANQAMSLVRKNEFLNQIKDELRKVISD
                     CNDRSVGTKISGILQRIDREVDSNKQREIFESAFDEVHEEFLQGVKTRYPELTPGELR
                     LCAYLKMNLPTKEIAPLLNISVRGVEICRYRIRKKMGITRDVNLTTHLLSI"
     gene            9525..10046
                     /locus_tag="PLA17_04870"
     CDS             9525..10046
                     /locus_tag="PLA17_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HPK84780.1"
                     /translation="MIKVHQNRIPNEFFVTKGSGCSEYAIHPGSYHMALHDAGISEYN
                     IMNYSSVLPATSRLVSLDEVDMPPFGSELCTIMAVANGFYGEHVSAGIIFAWLYTDDT
                     CEEKVGGLVCELAGYFDMEALEERLYTVLHDLHDRTFSQFYLGEPNVIRESLTIDKRY
                     GTALVSLCFVNFI"
     gene            10317..12149
                     /locus_tag="PLA17_04875"
     CDS             10317..12149
                     /locus_tag="PLA17_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPK84781.1"
                     /translation="MNDKRAICRTTLVLAFFIMTAMLCAQQRGSTHTFALGTSEFFLD
                     GKPFQIISGEIHPGRIPEEYWRHRIRMVKAMGCNTVSAYLFWNHHESEEGIYDFHTGN
                     RDIARFISTAAEEGMWVLIRPGPYVCAEWEFGGIPPYLLRNQGIKVRCMDPAYMKAAE
                     RYISRLADVLRPFLVTNGGPVLMIQIENEYGSYGNDRNYLIALKEIWERSGIDVPFFT
                     GDGPTTYMLEAGTLPGCAVGLDSGSSEKDFELARKMNPGVPVFSSETYPGWLTHWGED
                     WARPDTAELLREVKFLMDNRKSFNFYVIHGGTNFGFTAGANSGGKGYEPDLTSYDYDA
                     PIDEQGRATPKYMALRKLIGSYLPKREKLPSIPDPIPAMSFPETDLVPFTSVWNNLPE
                     PVLSVQPKPFEAYGQDYGFMLYSTRLTGHKRGKLTVIDLHDYATVFLDGKYVGTLDRR
                     LGINAIELPQPDSEYPLLEIFTEGMGRINFAHSMIDRKGITDRVILNGMTLMNWEVRG
                     FPMSDGYVESLEATATEMTRPGVFFRGTLKLDKRADTFIDMTNFVKGFVWVNGHNLGR
                     YWEIGPQTRLYCPASWLKDGENEIIVFDLHKTTPGSVRGFPAMN"
     gene            complement(12157..13203)
                     /locus_tag="PLA17_04880"
     CDS             complement(12157..13203)
                     /locus_tag="PLA17_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPK84782.1"
                     /translation="MDFSLLIIIIILAFAFDLINGFHDAANSIATIVSTKVLTPFQAV
                     LWAASFNWLAYLVFQLNVADTISKTVDTSAITLLVILSGILAAIIWNLTTWFLGIPSS
                     SSHTLVGGFSGAAIAYAGWNVVHTGVILKIGAFIFLAPIIGMLFAYIISVSLLWLCRR
                     ANPFRLEKKFKMGQLLSSALFSLGHGGNDAQKVMGIIAAALMVYFHGVDSSAVPRWAR
                     IEMVDGKMDSIPHWIVLGCYTAISLGTLMGGWRIVKTMGTKITKITPFEGVAAESAGA
                     LLLFGTEYFGIPVSTTHTITGAIMGVGLTKRITAVRWGVTINLLWAWIITIPISMAIA
                     AGVYHLLSAITGLQ"
     gene            complement(13215..13862)
                     /locus_tag="PLA17_04885"
     CDS             complement(13215..13862)
                     /locus_tag="PLA17_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPK84783.1"
                     /translation="MKLDKFFSFLVPKEGRFYPLFKDASANLIIGAELLVEFMNEPSP
                     EKREILGNKIKETELVGDQIARNLYNALSGTFITPFDREDIFELTHATDSVLDLIHGA
                     SKQARLYNLMEIPSEFREMADVIRKASIEINTAFNGLRDVLNLSKYKDNCQRIQELEE
                     QADDINHRYLAKLFQEEQDAIELIKKKDILAALEKAVDRCDDVSDILSSVIIKIA"
BASE COUNT         3619 a         3514 c         3274 g         3670 t
ORIGIN      
        1 caggtcattg taaactatca tcatgtcaag gagaaatggc acataggcag agttgttgtt
       61 agccaggaca atctgcttcg ttcctgtcag tactccaagg ctggtgttca gtttcgcagt
      121 cacattgtat gttcccttgc gcatgaagac atgctttaca acttcatcct tgcctgcggt
      181 gacttcatcg ctgccgtcac cgaaattcca tgttacatca gtaacttttt gcccttcagg
      241 aatggtgtaa ccggtaacag agcatgaaac ctcattgtca accccctctg ttgcatttac
      301 ggtgtatgta atagttggcc tgacataacc cttgggtatc aggtagtagt gccagttgtt
      361 ccatccctcg tatgattcga ggcaggtaag ctcgagaagg ttttcctcaa ttgtgcgtat
      421 ggtgtaggta ttatcctttg caccaacatc aaagcagggg aaaattggtt tttcaccatt
      481 cagggtgatg gtgtatttgc cttcggacac cttgatgata ttgaaggtgc ctgacaccgg
      541 tgttgcgtag tctacgtccc agtcaccctt gttttcacgt ggattgagat aaactgatgc
      601 cagtgccgga tcattggcac ggtaagcctt gacatagctt acgcccttat tgtcataggt
      661 aaccacgaaa ccgtcgagtt tgaaggtgat ctcatcgtcc atcaggccgg tgccatcctt
      721 gtcataggga gccgctgccc accagctcat tgctgtgggg tcatcaaccg ggccaacgcc
      781 cagatgaccg cgggtagcca catcaaggac ccacgtcttc ccggcagtat tgtcaatgcc
      841 tcctgtaaga ttaacataga caggatcagt gaagatggaa aagtcagttt cagtctgcgt
      901 cagggtcttc gtaatggtag ccattcctcc tcttgtgaca agtgtcatgg ctattgtata
      961 gtcacctggc atcgggtacc tggctatatt tgagttaccg gtagcttttg tgccgttacc
     1021 gaaatcccat gaagcgatac cagtgactga tgaagtgttt tcaatcacga agttaaacgc
     1081 atcgcttccg ggtgttatgg tgaagttgag ctgatcggct gtaggagcag cccctatttc
     1141 gggcttgtca gccatttgag gttcgcatgc attgacagcc agcacaacga ccatgatgcc
     1201 cgctaatctg cttatatatc tgattttttt cattacacta tttattattt aataattcat
     1261 aagccaatgt atgcgggaac catctgtttc agcactccgc tgttcatatt ccttatctca
     1321 ctggcgggaa tcgggaacat gccccatgaa tccggcttga aggttcttgg agcaaagtcc
     1381 gaagcgttgg gagtaccggc cgggatgttg aaactggcgt tgatcttctc tcctgcatag
     1441 tcaaggcctc tcctcagcag atcccatttt ctcagcccct cacctccgaa ttcaacgcgc
     1501 ctttcatgat agatgtcgtc aagggtgatg gttgtttttt cagctgcggc acctagtgcc
     1561 ctggtccgta cctggttgaa ataaccggca gccttgcccg gattgtcagt gagccatacc
     1621 tctgcagcca tgagcagcac gtcagcatac cttatatcca tgaagttacg ggggaagtta
     1681 tggttcgggt cacctcccga tccctggtaa tcagcccttg ccatgtattt cttgttgaaa
     1741 tagccggtat tctggaatgc cctgttataa tcagtcagca tcgcttcagc gtcatagagg
     1801 gtaacgtact tcctcgggtc tccaggttcg aactcgtcag caaggctcca ggtgggcact
     1861 gagaatgacc atccggggaa aacggtcggg tcaccctggg ggttacgaac gcttatccag
     1921 actgatgcga agttgccgtt gatattccag ccgccccagt cacctgactt ggctttgtct
     1981 gaatactgca tctcaagcac tgactcaaca gtgttctgat tatcccagtc aaacaggtca
     2041 gcataatcag gtatcagcga atagactccg ctgttgatta cctggtcgag agctgtctgt
     2101 acgtatgcct tattgatcac tgtggaaccg ttaccccact gctctgcagt cagtcccagg
     2161 ccgctgatag cgctcatacc ggtgtgatat aggtagatgc gggcaatcat agccttgacg
     2221 gcaccttttg tcacacggcc cttttctgca ggagtgacct ggttcgggag gacatcaact
     2281 gcatcaagca gatctgatgc aatgagtgta aatatctctg aaggagtgct ttgtgtaaga
     2341 ctcctgtaat cctcaaccga cgggagcacc tccgtcatca ccggaaccca gccgaaatga
     2401 cgtactaaat cccagtagaa ataggcacgc aggaatttaa cctcggcttc atacctctcc
     2461 ttgagacctt cactcttcca gtcaatgcca tcctgcttct gaagatagag gtttgccctg
     2521 tagacaccgg catagcagcg gctccataaa tctgaagatg aattattctc aggctccatc
     2581 ttgaacatct ccatatcctg ccactgactc atgtcagaag cgtcagaacc tccgcagaat
     2641 gcatcatctg accatatatc ggccattgtc gcggcaaact gccagttctg cagtgcatat
     2701 gcgtcataaa cagcagtcat ggccaggaag gcgtcagact ctgtcttgta gtagttggct
     2761 tccgtctgac cggtctttgg ttcgagctcc aggaaatccc tggaacagga tgagccggca
     2821 acaattacag ccagaaagaa aacactgtat aatattgact ttttcatagt tctgttcttt
     2881 tgaatgttag aaagtaacat ttatgccggt catgatcgta cgtggctgcg gataaatacc
     2941 gcgatcgatc ccgttgtcaa atactccgcc tccgatttca ggatcgtagc cggtatactt
     3001 tgtaaatgta tagaggttct cagccgacag atagaaacga agcttcttga gcttaatggc
     3061 tttggtgata ttctgcggga gagaataccc cagcgtaatg tttctaagtc tgacaaaact
     3121 tccgtcctga atgtagaagt cactgacggt tttatggttc aggttgttgt cagtaaaagt
     3181 gacacgcggg taatagttcg aagtgccggg accagtccag cggtcaagcc agtcagctgt
     3241 atagttggca tagttcatgt cataccgcct ggttgcgctg tatatctcct gacccagggc
     3301 tgcatagagg aacatgttga aatcaaagcc gtatatctca gcgttcaggt taagcccgcc
     3361 ggtgaagtca ggcaacggag agccgatctg gaccctgtca ccgtcactga ttgtaccatc
     3421 accattacta tcaataaact taatatcacc gggacttgca tttggctgta tcttttctcc
     3481 ggcacgattg acgtaggcgt caatctctcc ctggttctgg aagactccgt cagtcttgat
     3541 accaaaaaag acgcccatcg gaaaacctac ctgtacatat atcagttcgc tctggccaaa
     3601 accgccatca ccacccttca gaaccttatc agtattcttc aggtcaagga cttcactctt
     3661 gttaaaggca ccgtttactg aagctcccag gaagagcttg cctatctgct tacggtaaga
     3721 gacctgtgct tcgaagcccg agttcttaac ttcgcctccg ttgatcgtgg gagctccgtt
     3781 gccaacaagc attggaatgg gtgcagcaac ctgccagtcc ttggtcttct ttatatagta
     3841 gtcaagtgta acagtaagtg tgctgccaag ggttgtcata tcaagaccga tgttggtctg
     3901 ctctgatgct tcccatttga gcatgggatt ggccagcttg gtgggcaccg taccgttgta
     3961 ctgtgtcttt ccatcaccaa aatagtagat ggcgctgtta ccgataatgg aagtatagtt
     4021 aaagttgccg atattctcgt ttccgttctg accccagctt gccctgagtt tcaggacatt
     4081 gaatatatca gtaagattac tcatgaaatc ttcccttgag ataacccatc ctattgaggc
     4141 agaggggaag tatccatatt tgttttcgct tccaaacctc gaggatccgt ccttcctgat
     4201 tgtggcggta agcatgtagc ggtttttcag gtcatagttc aaacgggcaa agagcgagga
     4261 gacggtatgg tcactgtaac ctccgcctgc gttggcgctc tcagggtcag ttgcattgtc
     4321 aaggtatgaa tgctcaaagt catcaaagat cagatcattc ttcgtacccc agagattttc
     4381 atgcatatcc ttgaaggcag ttgtacctgc tatgatggtc aggttatgaa catcaaacgt
     4441 ttttgcatag gtaaacacgt tttcaaagtt ccaccttgac cacatgtcaa gtcccttgta
     4501 agccttgtca atattgctga agtgcatggc atcaaggtaa tactccggtg tatagctgtc
     4561 agtattgacc aatgcatatt cacccccgaa tgatgacctg aaagtgagtc ccctgagtgc
     4621 cggggatacc ttgccaaaat cgaactcgcc gtacaggttg ccgatcagct tgtaggttct
     4681 tgtccttgag ttattgtaac ttgccatggc cacagggttg gtgatctcct gcagaccaaa
     4741 tccgtatgcc tcggaagtac cccatgtacc gtcagaaaac atcaccggaa tcaccggtgg
     4801 catgttaagg gcctggttca gaccaagacc atacctgtca ttggtcgcaa tacccttgct
     4861 ggttattccc gcaaggttga catttcctcc cagcttgaag agaccaaagg tggcagctgc
     4921 attcagcctg tagcctactt tctcaaagct tgacttgcct ttggaaacta taccttcctg
     4981 gctgaaatag ttcagcgagg atgaatactc cagtctgtca ctgccgccgg tgaaagaaag
     5041 agagtggttc tgctttggag cattgtagta aaacatctca tcctgccagt cagtatctgc
     5101 tgtaaagccc tctatctgtg cctggctgaa acgtggcccg agacctgcat tgactgatgc
     5161 ctcattgata agcattatgt attcacgtga attcagcaga ggcatctttt tccagggatt
     5221 ctgaaaaccg taataaccgt cataggtcac tgtcagttta gtgtccgtac gcccggtctt
     5281 tgtggttata agaacaaccc cgtttgctcc gcgtgcacca taaattgcag cagatgcggc
     5341 atctttcagc acctctattg actcaatgtc acttgcattg agaaagtcag taccggcacc
     5401 gctcatcggc aatccgtcaa taatatacaa aggttccgca tcaccgtagg tgcctgcccc
     5461 cctgatacgt accgagatct gagagcccgg ctgaccagag ttggccgtga tgaccacacc
     5521 ggccgtcttg ccctgaagtg cctgcgttac acgcatgtca gtggccttgc gcaggtcatc
     5581 accctcaacc ttcgcaattg caccggtgac aaggctcttc ttctgagtgc catatcccac
     5641 aacaacaacc tcgtcaatca attcaatatc agaggccaat acaatgttaa tggtggtgct
     5701 ctcagtcacc acaacttcct gtgccttcat ccctacaaat gagaagacaa gaagtgagtt
     5761 catgggtaca ttgtcgagac tgtagtaccc gttcatgtca gtcatagttc cggtggtggt
     5821 tcccttgact aaaacactta ctcccggaac aggctgtccg tcgtctgctg acgttacaac
     5881 tcctttaatc tgcagcccct gagcatttaa agccagggaa caggcaatga aaaaaagagc
     5941 caggattgtt tgtttcataa acgttgagtt tttgagtttg gttatcaggt tttcagatga
     6001 ttataaaacc acccgtttgc aaggagcaag ttaagaaaga gaaattgatt atcacttgtt
     6061 aaaatttact tcaaatatac atcagtcatt tttttaaaat acatcattaa ttcatcattg
     6121 aaatcacata atgttgattt acagatacat aataaacact tattgagtgt ataaaataca
     6181 ccaattacca gaagcaatac tacagcgccg cgttttttca aatactgaga agatgggttg
     6241 tgaggtttac atcacgggta atgcccatct ttttcctgat cctgtacctg catatctcaa
     6301 cacctcttac tgatatattg agcagaggag caatctcctt ggtcgggaga ttcattttaa
     6361 ggtatgcgca caatctcagt tcgccgggag tcagttcggg atatcttgtt tttacaccct
     6421 gaagaaactc ctcgtgtacc tcatcaaagg cactctcaaa tatctctctc tgcttattgc
     6481 tgtcaacctc ccggtctatc cgctgcagaa tcccggaaat cttggtgccg acagacctgt
     6541 cattgcaatc actgatcact ttcctcagct cgtccttgat ctggttgaga aattcattct
     6601 tacgcacaag actcatagcc tggttagcca gctccttgtc acgaagtatc atctcagcct
     6661 gaagcctttc attcctgatc ctgattatct ccttctcaga ctccagcgcc tgccttttgt
     6721 actcctgttc tttctcctcg tagatacggg cgttctttac tctttcattc cttcttgaga
     6781 catctatcct ccagctgata aaacgggcaa gaagcagcac catgattatt cctgcaatga
     6841 cataggcgat aattgccacc ggcgacttat accagggatg ctctataaca aactcaaggg
     6901 agtcaggttt gctctcaatt ccgagctgat tcctggcttt tacatggaag gtgaatttcc
     6961 cgtcagggag atttgaatac tccttatagt atgtacggct ccagtcagac catctgtcat
     7021 catacccttc cagcctgtaa ctgtattcgg tattgccggc attgtcatat accggcgaag
     7081 caaacatgaa cctcatgttg ttgttggcat atctgaatct gtagcttatc tcccggactg
     7141 cagatgaagg agtgcggcca taataaaata ccgagtcgtg atgcagtgcc tctccaaggg
     7201 taatgtaaac cgagaaccct tcatatccaa gtgaagaggt acgcgatgaa tagtgggcaa
     7261 aaccatcttc cgtgcctata atcacatgct ccggagcata tgggtaaaaa gactcaaacc
     7321 cgtggatgaa ccctcccgag agcaggagaa aaggagtggc aatatgctca taggtgtgat
     7381 cttccttcag cctgaatacc ccagccctgt tctttgccac ataccatatg ttcccctctt
     7441 tgtcctcatg gagatgtgag acagccacca ggggactgaa aagctggttg aagtaggttg
     7501 acctgacaaa ccggtctgtt tcaggcgaat attcatagat ctcagtctct gataccacca
     7561 cgagttgtcc gaggacacga aaaataccaa gatagaaatc cgatggcagg ccatctgagg
     7621 aatcatagaa cctgacgctg gtcaccgaat cgagctctgc cgagagcctc accctgaata
     7681 tccccttgaa tccgtgactc atccatatct ctccctctct gtcttcctcg aaaaccctga
     7741 acgactcggt catacccggt atctgcctga caaacttcca cgtgccctgc tcccacctta
     7801 aaagtatcat tccgctgaat gtacctccca gcaggtatcc cgggtggttt ttcagttcca
     7861 tgtatttcca tccgccggag actcggctga taaggtcagc cttctctccg gagaccagat
     7921 aggtgccgtt attatgcccg cagagcagta taccgttgtg aactcccagg taccacacct
     7981 gcccgtaggt tccgggtatc atccgaaaat cgtcacccgt ctccctttct gaccagttcc
     8041 ttacatacag cccctgattt gttccgaggt agagctttcc atcatggata accgatgcat
     8101 atcctgcgcc aaagccttcc gtgctgcgga taaaggtaac aggggaatta atggttacat
     8161 agtcgattcc gttgtcaagt gccagccaga ggttaccgtt cctgtcggtg aacagggcaa
     8221 gaaccgtgtt gttctgcaga ccattcttcc tgttgatgtg ctgaacaatt ctcccctccg
     8281 ggtcaataat catcacgccg ttaaggattg tgcccatcac gtaataatca tcatatatca
     8341 ttgtagcgct gaatacctgg tcacggctca gtatactgct gagtgcactg tcccactttc
     8401 gaagcttcgc gccgtcataa ataaacagac cgttccccag ggtgcagatc atcatctcat
     8461 tcccggaccc gtaaggaaga agtgagcaga tatccattcc ggtcagcgac tcgcagccgg
     8521 ggagtcttgc aagccgggtt ccgtcgaact ccataagtcc gtattcggta tcattgaaca
     8581 gcagcctgcc ccttacagtg taagaattct ggaaccgtga cggagcagcc acaactgtca
     8641 taggtgtgcc atcacggtaa aaataggctg cataatatga ctggaacaca gttttgtcgc
     8701 ggtatgtgaa tatgttccat acatcgtcaa aattcctgta ctcctcgggt atcagttccc
     8761 tgaaggattg gaaagagaga ttaccgccag cttcaggcac catttttccg aagtcattat
     8821 aggctccaat gaatatctcc ccctttgcac ccatcagaat tgacctgacc attgagtagt
     8881 tgggcatccg gtaaaggtgc cactgaaccc cgtcatagac caggagtccg tcattattgg
     8941 caaaatacat gaatcccctt gcatcctgcg ccacagccca gttctgggtg ctggcacggt
     9001 agtcggcctt tgtgaagttc ctgatgaacg gcgtgccaat ctcctgaacc tcagctgttg
     9061 cattataaac tgcgggaagg agaaaaaaca ccactgaaat caacctcctg atcatatcat
     9121 gtttttaagt cctacaattt atgttttttt tggcagcttc ccgcgaataa aacctgacag
     9181 cgcttcaagg gaatgaaaac tgatattgct tccctggaat gagaactgat aatgcttcac
     9241 aggaatgaaa cctgatactg cttccctgga atgagaactg ataccgcatc gcgggaataa
     9301 aacctgacag cgccgggaaa atgtatgtca ccgcaacagt gatcagatac tcccgcgtcc
     9361 attctataac aagtgtctga aacataacat cccttcgcgg atcctgcaaa cccatacccg
     9421 tccgaaccga ttatgccgca gtatttatgt tgattcttta tttttggtat attcgcctgg
     9481 tccggtctta tgaagggtat aaatctaaag tattgaaaaa caaaatgatt aaagtccacc
     9541 aaaacagaat tcccaacgag tttttcgtta ccaagggttc aggatgcagc gaatatgcca
     9601 tccatcccgg ttcatatcac atggcactgc atgatgcagg catatcggag tacaacatta
     9661 tgaactattc ttcggtgctg cctgcaacct caaggctggt gtcacttgac gaggttgaca
     9721 tgcccccatt cggctccgaa ctctgtacga tcatggcagt agccaacgga ttctacggtg
     9781 agcacgtgtc agccggaatc atattcgcgt ggctctacac ggatgacacc tgtgaggaaa
     9841 aggtcggagg cctcgtctgt gaactggccg gctactttga tatggaagct cttgaagaga
     9901 gactctacac tgttctccat gacctgcatg acagaacctt ctcacagttt tacctgggag
     9961 aaccaaacgt catcagggag tctctgacaa ttgacaagag gtacggaact gcactcgtat
    10021 ctctctgctt cgtcaatttc atctgatctt tccggagcat agaagagagg agagtctgat
    10081 tcatctgatc gttccggagc atagaagaga ggagagtctg attcatctga tcgttccgga
    10141 gcatacaagg gaggagattc tgatcttaaa ctgagtgggc aaaagtccac tgccacaacc
    10201 agggaaacat gtccctgacg ataatccgcc cggcactcag gaagacttac ggaggcagga
    10261 taaaacagaa cagtctgaga agcatttcat attgtaacca atatatatta caggccatga
    10321 atgacaaacg tgctatttgt cgtacaacac ttgttctggc attcttcatt atgactgcca
    10381 tgctttgtgc gcagcaacgc ggttcaacgc acacctttgc actgggaacc agtgagtttt
    10441 tccttgacgg gaaaccgttc cagattatca gcggtgagat acaccccgga aggatccctg
    10501 aggagtactg gcgtcacagg atcaggatgg taaaggcgat ggggtgtaat accgtctctg
    10561 cgtacctttt ctggaaccat catgaaagcg aagagggaat ctatgacttc cacacaggca
    10621 accgtgatat cgcaaggttc atctccactg cagccgagga ggggatgtgg gttctcataa
    10681 ggcccgggcc ctatgtatgc gctgaatggg agtttggcgg aatacctccg tacctgcttc
    10741 gcaatcaggg cataaaagta cgctgcatgg atccggccta catgaaagct gcagagagat
    10801 acatatcacg gctggctgat gtgcttcgcc ccttcctggt gacaaacgga ggtccggtgc
    10861 tgatgatcca gattgaaaat gaatacggaa gctacggcaa tgaccgcaac tacctcatcg
    10921 ccctgaaaga gatatgggaa cggagcggta ttgatgtccc gttttttacg ggtgacgggc
    10981 ccacaacata catgctcgag gcaggaacgc taccgggatg tgccgtggga ctcgactccg
    11041 ggagctcgga gaaggatttc gaacttgccc gtaaaatgaa tccgggagtc cctgtcttct
    11101 ccagcgaaac ataccccgga tggctcaccc actggggaga agattgggca aggcccgaca
    11161 ctgccgaact gctgcgagaa gtcaagttcc tgatggataa ccggaaatca ttcaattttt
    11221 atgtcattca cggaggaaca aacttcgggt tcacggcagg ggccaactca ggcgggaagg
    11281 gatacgagcc tgatctgacc agctatgact atgatgcgcc aatagatgag cagggaaggg
    11341 caaccccgaa atacatggca ctcagaaaac tgatcggctc atacctccca aaaagagaaa
    11401 aactgccgtc aataccggat ccaatacctg ccatgtcttt cccggagact gacctggtgc
    11461 cattcacatc tgtctggaac aacttgcctg aaccggtatt gtcagttcag ccaaaaccgt
    11521 ttgaagcata cgggcaggat tacgggttca tgctgtacag caccaggctg acgggccaca
    11581 agagaggcaa actgacagta attgacctgc atgactatgc cactgttttt cttgacggaa
    11641 aatatgtcgg gacacttgac aggcgactgg gaatcaatgc aattgaactg cctcagccgg
    11701 attcagagta cccgctgctg gaaatattta ccgaagggat gggaagaatc aactttgccc
    11761 attcaatgat tgaccgcaag ggaataactg accgtgtgat actgaacggg atgacgctga
    11821 tgaactggga ggtacggggt tttccgatga gtgacggcta tgttgaatcg ctggaggcca
    11881 ccgctaccga aatgaccagg cccggagtat tcttcagggg caccctgaaa cttgacaaga
    11941 gagcagatac ctttattgat atgaccaatt ttgtcaaggg gtttgtttgg gtcaacggcc
    12001 ataacctggg aagatactgg gagatcggtc cgcagacacg gctttactgc ccggcatcat
    12061 ggcttaagga tggggaaaat gaaataatag tatttgatct tcacaagacc acgcccgggt
    12121 cggtaagggg attcccggcc atgaactgac aactggtcat tgcaggccgg ttattgctga
    12181 caacaggtgg tagaccccgg cagcaattgc catcgatata ggtattgtaa ttatccatgc
    12241 ccaaagcagg ttgatagtta caccccatct tacagccgta atcctttttg tcaggcctac
    12301 ccccatgatt gctccggtaa ttgtgtgggt tgtgcttaca ggtataccga aatactcagt
    12361 tccgaacagc agcagtgctc cggccgattc agcagcaaca ccctcaaacg gagttatctt
    12421 ggttatcttg gtacccatcg tcttgacgat cctccagccg cccataagtg ttccaaggga
    12481 gattgcagta tagcaaccca gcactatcca gtgaggtatt gaatccatct tgccgtcaac
    12541 catttcgatc ctcgcccagc gcggaacggc tgatgaatca actccatgga aatagaccat
    12601 gagtgcagcg gcaatgatcc ccataacctt ctgtgcatca tttccaccgt ggccaaggct
    12661 gaaaagggct gatgaaagga gctggcccat cttgaacttc ttctccagcc tgaagggatt
    12721 cgccctccgg cacagccaga gcagtgaaac agaaatgata tatgcaaaca acatcccgat
    12781 aatcggagcc agaaagataa aggcacctat cttcaaaatc acacctgtat gcaccacatt
    12841 ccatccggca tacgctattg cagcaccgga aaagcctccc acgagggtgt gtgacgaact
    12901 ggagggaatg ccgaggaacc atgtcgtgag gttccagatg atggcagcca gaatgccaga
    12961 caatataacc agaagcgtaa tagctgaggt atcaaccgtt tttgaaatgg tatcagccac
    13021 attgagttga aaaacaaggt acgcgagcca gttgaatgat gcagcccata acaccgcctg
    13081 gaatggtgtg agcacctttg ttgatacaat cgtagctatt gagttcgccg catcgtgaaa
    13141 cccgtttatc aggtcaaatg cgaacgcaag aatgattata atgatgagaa gtgaaaaatc
    13201 catctgcagg ccggttacgc gatctttata atcacagaag aaagaatatc agacacatcg
    13261 tcgcaacggt caacagcctt ctcaagggct gccagaatat ccttcttttt aatcagctca
    13321 atcgcatcct gctcttcctg gaaaagtttt gccaggtatc ggtggttgat gtcatcggcc
    13381 tgctcctcaa gctcctggat cctctggcaa ttatctttgt atttcgaaag gttgagcaca
    13441 tcgcgcaacc cgttgaaagc tgtgttgatc tcaatggagg ccttcctgat gacgtcagcc
    13501 atctcccgga actctgatgg aatttccatg agattataca ggcgggcctg ctttgatgca
    13561 ccgtggatca ggtcaagcac agagtccgtg gcatgggtca gttcaaaaat atcctccctg
    13621 tcaaagggtg tgataaaggt gccgctcagg gcattgtaaa ggtttctggc tatctggtct
    13681 ccgacaagct ccgtctcctt tattttattg ccaagaatct ctctcttctc cggagagggc
    13741 tcattcatga actccaccag caactctgca cctatgataa ggttggctga tgcatccttg
    13801 aaaagtggat agaacctgcc ctccttggga accaggaatg aaaagaactt gtcaagtttc
    13861 atcagtaagt gtaaaatatg ttaacgcctg ccaaatgtat attaatgact tcactaatta
    13921 aagaagtttt gatataatgt tcattaaatg ttacaaaatt gtttcaggca ggattctggc
    13981 aactttcgct atattagcag attatttaaa aaccggtcaa aaggcatgaa gctgacattt
    14041 tacggagctg caagggaggt gacaggcagt aagatac
//
LOCUS       DAOPBK010000058        14007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4370_length_14007_cov_5.523724, whole genome shotgun sequence.
ACCESSION   DAOPBK010000058 DAOPBK010000000
VERSION     DAOPBK010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4370_length_14007_cov_5.523724"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLA17_04890"
     CDS             complement(<1..240)
                     /locus_tag="PLA17_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPK84784.1"
                     /translation="MTNKRKIINDPVYGFISVPNEFIYDLISHQWFQRLRNIRQLGLS
                     SFVYPGAVHSRFQHSLGAMYLTGQAVHTLRSKGIEI"
     gene            445..894
                     /locus_tag="PLA17_04895"
     CDS             445..894
                     /locus_tag="PLA17_04895"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84785.1"
                     /translation="MITKDHIRRLVEGHISGTGIFPVDVRLSSTGRITVLIDRMDGVT
                     IDDCAMLSRQISNDLGEEAGNYELNVSSPGLDMPLLVPQQFIKNEGRMVDVVTNEGER
                     FKGIMMNVTAGGFDLETEKKRGKEIAGTVRSFNFEDVKSVKVSISFK"
     gene            911..2155
                     /gene="nusA"
                     /locus_tag="PLA17_04900"
     CDS             911..2155
                     /gene="nusA"
                     /locus_tag="PLA17_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPK84786.1"
                     /translation="MENVNLIDTFSEFKELKNIDRPTMMSVLEDVFRGMLAKKYGSAD
                     NFDIIVNIDKGDFEIWRNREVVDDEDFTDATTQIPLSEAHKIDEDYEVGEEVSDEVRF
                     LDFGRRAILALRQNLVARILDLEKNNIYLKYKDRIGDIVTGEVYQVWKRETLVLDDDG
                     NELILPKPEQIPSDHFRKGDTIRAVVIRVEMKNNTPNIILSRTSPVFLERLFELEVPE
                     IFDGLITIRKIVRIPGERAKVAVESYDERVDPVGACVGMKGSRIHGIVRELRNENIDV
                     INYTSNIQLFITRALNPAKISSIKLIESEKKAEIYLKPSEVSLAIGKGGLNIKLAGQL
                     TGYDIEVYREPDNEDEEDVNLDEFADEIEGWIIDELKAIGCDTARSVLRLTIHDLVKR
                     TDLEEETIREVVNILKSEFEKE"
     gene            2214..5117
                     /gene="infB"
                     /locus_tag="PLA17_04905"
     CDS             2214..5117
                     /gene="infB"
                     /locus_tag="PLA17_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPK84787.1"
                     /translation="MTEEKKAIRLSKAAREFNVGIQTIVEFLNKKGFDISTDPNGKIP
                     HEAYALIVKEYSSDLSVKKDAEKLALKEKITPKRSLSIEDMESGRDGDNGEPDSDFLI
                     TDISGIGIKKHIDLRTEARKPEVRTEDKSDLTPPAKPVKKEKKAPVAAPSPEPAVTTT
                     TEAPVAAEVPETPAGPRKPKVVGKVDLDKLTKPEKPSKKAAGKKKEAAPEVRKQEPEA
                     PRPEPEPVVTAPEPEPPVTQEPKETEIFRPGFEKLTGPKIIDKIKLEPEVKKSRDQST
                     GDKKKHSRKRERIKAKPREKVALDTHVAPSPQETRKEKKGSQKDHRGDRKKSYRALRP
                     EVDREDVEKNIRDVNLLMSKSKSRVAKYRRDKRDAISQRMKDETEKRELEKSVLKVTE
                     FVSVNEMATMMDVPVVDIISTCMSLGLIVSINQRLDAETMALVAEEFGFTVEFVSAEL
                     QEAVKEEEDDESKLRPRPPIVTVMGHVDHGKTKLLDYIRNTNVIAGEAGGITQHIGAY
                     SVTLEGEKIITFLDTPGHEAFTAMRARGAQITDIVIIVVAADDGVMPQTREAMNHAHA
                     AGVPIIFAINKIDKPTANPEKIREELANMNFLVEEWGGKYQSQDISAKNGINIDLLLE
                     KILLQAELLELKANPDKLANGTVLESSLDKGRGFVATVLVKAGTLNVGDVVIAGSCFG
                     NVKAMYNERNQKISKAGPSMPVLILGLNGAPQAGDRFNVMESEKEAKDIATKRAQLQR
                     EQDLRTQKHITLDEIGRRIAIGNFQELNIIVKGDVDGSVEALSDSLIKLSTEEIQINI
                     IHKAVGQISESDILLAAASNAIIVGFQVRPSLSVRKLAEKEGIDIRLYSIIYDAIEEI
                     KAAMEGMLSPEIKEEVVATVEVREIFKIGKVGTVAGCFVRDGKISRNTKVRVIRDGIV
                     IYTGDLGSLKRFKDDVREVTSGYECGLNIENFNDIKAGDVVEGYQTVQIKKTL"
     gene            complement(5383..5757)
                     /gene="gcvH"
                     /locus_tag="PLA17_04910"
     CDS             complement(5383..5757)
                     /gene="gcvH"
                     /locus_tag="PLA17_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPK84788.1"
                     /translation="MNLPTNLKYTKDHEWVLIKGNEATVGITDFAQHELGDIVFVEIE
                     TVGEELDHGEVFGTVEAVKTVSDLFMPLSGTVKEKNEELDTAAELVNSDPYGKGWMIR
                     IELSNPSEIDELLDAEGYKKLL"
     gene            complement(5871..13226)
                     /gene="sprA"
                     /locus_tag="PLA17_04915"
     CDS             complement(5871..13226)
                     /gene="sprA"
                     /locus_tag="PLA17_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPK84789.1"
                     /translation="MLFSLSSLVIKAQTETDNSLLRLNYDPAFPWEAKKSSPLFLYNP
                     SNITTRVEYDGRNNQYIIYQKAGTLDFRKPVYMSAEEYRRFEFNQAMREYWDASLRGD
                     ATGYRSSLIPQIEVGGETFDRIFGSNIINIVPQGSAELIFGINISHTENPTISERLRT
                     IPTFDFQEKIQMNVTGTIGDKMQLGINYNTEAMFEFENRTKLEYSGKEDEIIKKIEAG
                     DVTLPLNGTLITGSYSLFGLKTELQFGKLTMTTVFSQQKGESSVVEVKGGSQISDYEI
                     TADNYEANRHFFLSQEFRNNFDGALSNLPIVSTGLNIEKIEVWITNQTSRFEDVSNRN
                     IIAFLDLAENQTNIFNSVPEFQGIPGTPLNPSNNTNGLYEQLNTTYSNVRNIDQVADA
                     FAGLYPGFQIGRDYEKIENARKLTEREYTVNRQLGYISLNVALNNDEVLAVAYEYTLN
                     GQVFKVGEFSTDGITAPDALILKLIKGTSLSPQIPTWKLMMKNIYNIGSGRIEPKNFE
                     VNILYQDDKTGNALNYLPGTPLDGQVLLQALGLDQLNSQLDRQPDGLFDFVDGITVMA
                     ERGRIIFPVLEPFGRHLLKYITDPEQIRKYVFTELYDSTQTVARQMAEKNKYLLRGQF
                     TSASGSEIRLNVANIPEGSVKVTAGGVTLTENVDYTVDYNMGSVKIINSAIIESQTPV
                     QVSLESNQFFGLQTKTLLGTHLNYRFSDKFNLGGTVLRLTERPYTQKVTYGDDPISNT
                     IYGFDASYRSESQLLTNVINKLPFIKTNTPSSINFFGEFAHLVPGHSRAITSEGAVYI
                     DDFEASEISLDLRAFNAWSLASIPQGLDHYFPEARLSKDLRSGINRARLAWYVIDPLF
                     LRNGSTTPGHIRSNPDTQSSHFVREIYETEIFPYKESPSGIPTNISVLNVAFYPEERG
                     PYNFDAQAGEYSAGLNYDGLLNSPRTRWGGMMRELLTNDFESANIQYIKFWLMDPFVE
                     NPDHEGGDLYFNLGNISEDILRDSRKQFENGLPTSPLVVNVDTTVWGRVPTVQAVVHA
                     FDNNIESRRYQDVGLDGLSNEDERTFFDSYLQAVGAVVSPEILEKILQDPANDDFHYF
                     RGSDYDLLQLGILERYKNFNGTEGNSPTSEMSDESYPTSGSTLPDTEDINRDNTLSET
                     ESYYQYHVSLRPEDMVVGRNFIVDQLEYTATFANGEKSPVKWYQFKIPITDYERIVGS
                     IRDFKSIRFLRMYMRNFDEHVIMRFARLELIRSEWRKYNLSFFEGGERITVPEEDDGS
                     FEISSVSIEENAGKEPVNYVLPPGFDRVIDPSNPHLRQLNEQSMVLRVRDLADGDARA
                     TYKNVALDMRQYRRLKMEVHAEALIGEPLQDDELTVFVRLGSDYKNNFYEYEIPVKLT
                     PYGRYSNESEEQRAVVWPELNKIDIDLSLLLDAKQSRDAAMRAPGSVVSEADIWSVHD
                     GIARISVSGNPNLSNVRVVMVGVRNPIRTRRPQTDDGAPLSAEIWVNELRLADFREDG
                     GWAANAQMQARLADLGTINLVGQTSTPGWGSIDKNVSQRSMEQVIQYDLSSNLELGKF
                     FPEKAGIRIPVYMGYSENRIRPQYDPLDPDILLNDALDNLGSKEERDSVLNLSEEYSR
                     RRTFTVSNAGTTWRGEKPHAWDPANVTVSYAFNEVYNTDPRTEIDVERTYRGGIAYDF
                     EARPKNFTPFQKSGLFSSSVFRLIKDFNIYLFPKHITVRTDLYRYYNEIRTRNINNPN
                     LLIKPVFTKDFQWNRFYDIKYDITKQLKVDFTASSIARIDEPEGGVDRRRYPELFDAW
                     RDSIMTNLASFGRPTSYYHLLNLNYNIPVNKLPLLSWLTSNARYSARYDWLAGTIFPD
                     SLNINPGNTIKNSNNSQFTLQANLTNLYSKVKFLKEVEASTQPGAVRRMSLGYEEVTH
                     SRRGLNFKAGKPRIIIHNLKTKDVTVTVNAPDGTVVSGKFDVVSDVRIDFTADADVTN
                     ASVDIKGRVEKKPGIASVAGKYLVRGLMALRNVSATYTVEQGHILPGYLPGTTFLGQQ
                     NYGGMSSPGWRFLVGLTDERFFDEAVINGWITTDTLLNNAAAFSKREQINMRANLEPF
                     PGFRIDITGDRRYSETISSYLRADLNGNFPDSTRNTRITGNFTISVVSWGTAFEKIPG
                     SGDYNSGTFERFREYTAIISQRRAQERSRIDNSYDPGFDPVTGEPVTGPYASGYGLTS
                     AEVLVPAFLAAYTKRDPDKISLSPFPSILHIMPNWRINFEGLTRFEAVRKIFNSVSLS
                     HQYRSTYTIGSFNTSLYYDPDESGISRIRDLKSNFIPQYEINTVTINEQFSPFINIDL
                     GWKNSLTTRIEYRKSRTVTLNLTSNQVADIRNDEITIGAGYRFDDVAITLRSRTGQRA
                     LQSDLNIKLDLSIRDNKTLARKLIEEVNQPVAGQRVFTLGATADYVLSDRFNLQIYAD
                     HTMNDPFVANTYLTSNTNFGFSLRFTLVQ"
     gene            complement(13291..13872)
                     /gene="ruvA"
                     /locus_tag="PLA17_04920"
     CDS             complement(13291..13872)
                     /gene="ruvA"
                     /locus_tag="PLA17_04920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPK84790.1"
                     /translation="MIDYITGTVTELNPACAVVECNGIGYDINISLNTFSALEKDASC
                     KILIHEAIRDDAHLLYGFATADERNLFRQLITVSGVGAGTARMMLSSLTPSDLREAII
                     SGNVAILKGVRGIGLKTAQRIIVDLKDKIGKHAGAGEILTFSDNTAREEALSALVMLG
                     FARSSVLKVIDNILRENNKLQVEEIVRKALKLL"
BASE COUNT         3501 a         3423 c         3656 g         3427 t
ORIGIN      
        1 aatctcaata cccttgctcc tgagtgtgtg aacagcctgc ccggtcaggt acattgcccc
       61 gaggctgtgc tgaaaacggc tgtgtaccgc accggggtag acaaagcttg agagcccgag
      121 ctgcctgatg ttgcgcaacc gctgaaacca ctgatgactt atgaggtcat agataaactc
      181 attcggtaca ctgatgaatc cgtataccgg gtcattgata atctttctct tgttagtcaa
      241 acagcatgga ttttatggag acaaatttat tcaatagttt tcataaatgg gaaagaaaag
      301 cttcatatac agttctccag acacatttta gtataatcta tccattgata attgaaaaaa
      361 tggttttatc tttgcacccg accagaaggg tacgattggc ctaggggaca aaaagtcccc
      421 tattttgtta tcaaaaatca gactatgatt acaaaggacc atatcaggag acttgtggaa
      481 gggcacatca gcggcaccgg catattcccc gttgatgtca ggctgagcag caccggacgc
      541 attaccgttc tcattgacag gatggatggt gttaccattg atgactgcgc catgctgagc
      601 aggcagataa gcaatgacct cggcgaggaa gccggcaatt atgaactgaa cgtctcgtct
      661 cccggactcg atatgcccct gctggtgccg cagcagttca taaagaacga gggcagaatg
      721 gttgatgtgg tcaccaatga gggtgaacgc tttaaaggga taatgatgaa tgttacggca
      781 ggcgggtttg acctggaaac tgaaaaaaag agagggaagg agattgccgg aacggtcaga
      841 tcatttaatt ttgaagatgt taagtcagta aaagtaagta tatcattcaa ataaagtaca
      901 gattacaaca atggaaaatg taaatctcat agatactttt tcggagttca aggagctgaa
      961 gaatattgac aggcccacca tgatgagtgt gctcgaggat gtattcaggg gcatgcttgc
     1021 caaaaaatac ggttctgctg acaattttga catcatcgtc aacatagaca aaggcgactt
     1081 tgaaatctgg cgcaaccgcg aagtagttga tgatgaggat tttactgatg caactaccca
     1141 gataccgctc tccgaagccc acaagattga cgaggactac gaggtgggtg aagaggtatc
     1201 agacgaggtc agatttcttg atttcgggcg cagggcgata ctggctctca ggcagaatct
     1261 ggtggcacgt atccttgacc tggaaaagaa caacatttac cttaaataca aggaccgcat
     1321 cggtgatatc gtcaccggcg aagtttacca ggtgtggaaa cgcgagaccc ttgtccttga
     1381 tgatgacggc aacgagctta tacttcccaa accggagcag attccctctg accacttccg
     1441 caagggtgat acgatacgtg ccgtggttat cagggttgaa atgaaaaaca acacgccgaa
     1501 cattattctg agccgtacct ctcccgtgtt cctggagaga ctctttgagc ttgaggttcc
     1561 cgagatcttt gacggcctga tcacaatccg caagatcgtc aggattcccg gcgaaagagc
     1621 caaagtggct gttgaatcat atgacgagcg cgttgacccg gttggcgcat gcgttggcat
     1681 gaagggatca cgtatacacg gtatcgttcg cgagctgcgt aatgaaaaca tcgatgttat
     1741 caattacaca tcgaacatac agctctttat caccagggca ctcaacccgg ccaagatatc
     1801 ttcaataaaa ctcatcgagt ctgagaagaa ggccgagatt tacctcaagc cgagcgaagt
     1861 atcactggca atcggaaagg gaggtctgaa catcaaactt gccggtcagc ttaccggata
     1921 tgacatcgag gtttaccgcg aaccggataa cgaggatgag gaagacgtca accttgatga
     1981 gtttgcagat gagatagaag gatggattat tgatgagctg aaagccatcg gctgtgacac
     2041 ggcacgaagc gtactgaggc tgaccatcca tgacctggta aagagaactg atcttgagga
     2101 ggagacaatc agggaggttg tcaatatcct caaatctgaa tttgaaaagg aatagcctgc
     2161 aacctcaagg caataatata ttaacttaaa gagaggttac tataaagtga tatatgactg
     2221 aagaaaagaa agccatacgg ttaagcaagg cagcccgcga gttcaatgtc gggattcaga
     2281 ccattgttga gttccttaac aaaaagggat ttgacatctc cactgatccc aacggaaaga
     2341 tcccgcatga ggcttacgct ctgatagtca aggaatacag cagcgacctc agtgtcaaga
     2401 aggatgccga gaaactggct ctcaaggaaa agatcactcc aaagaggtct ctctccatcg
     2461 aagatatgga gtcaggcagg gatggtgaca acggggagcc tgacagcgat ttccttatca
     2521 ctgacatttc cgggatcggg attaagaagc atattgacct ccggaccgag gcacgcaagc
     2581 ctgaggtcag gactgaagac aagagtgacc tcactcctcc cgcaaaaccg gtgaagaagg
     2641 agaagaaggc tccagttgct gctccttctc cggaaccggc tgtcacaaca acgaccgaag
     2701 cgcccgttgc ggctgaagtg ccagaaacac ctgccgggcc caggaaaccc aaggtggtgg
     2761 gcaaggttga tcttgacaaa ctgaccaagc ccgagaaacc gtcgaaaaag gccgccggga
     2821 agaaaaaaga agctgcacct gaagtcagga agcaggagcc tgaggcaccc cgccccgaac
     2881 cggagcctgt tgtgactgca ccggagcctg aacccccggt gacccaggaa ccaaaagaaa
     2941 cagagatatt ccgcccggga tttgaaaaac tcaccggccc gaagatcata gataagataa
     3001 agctcgaacc tgaagtcaaa aagagccgtg accagtcaac tggtgacaag aagaagcata
     3061 gcaggaagag ggagaggatc aaggccaagc cacgcgagaa ggtggccctt gacacccatg
     3121 ttgccccttc gccgcaggaa accaggaaag agaagaaggg atcacagaag gatcaccgcg
     3181 gcgacaggaa aaagtcgtac cgcgccctga gacctgaggt tgacagggaa gacgtcgaga
     3241 agaatatcag ggatgtcaac ctcctgatga gcaagagcaa gtcgagggtg gccaagtacc
     3301 gccgtgacaa gcgcgatgcc atcagccaga ggatgaagga cgagaccgaa aagagggagc
     3361 ttgaaaagag cgttctcaag gttacggagt tcgtttcggt taatgagatg gcaacaatga
     3421 tggatgtgcc tgtggttgat atcatctcca cctgtatgag cctcggactt attgtctcta
     3481 taaaccagag gcttgacgca gaaaccatgg cgctggtagc tgaggagttc ggattcacgg
     3541 tagagttcgt cagcgccgag ttgcaggagg cagtgaagga ggaggaagat gatgaaagca
     3601 agctccggcc gcggcccccg attgtcactg tgatgggaca cgttgaccat ggcaagacca
     3661 agctccttga ctatatccgc aacaccaacg tgatagccgg tgaggctggc ggcataacac
     3721 agcatatcgg agcctattcc gttactcttg agggagaaaa gatcattaca ttccttgaca
     3781 ctcccggtca tgaagcattt accgccatgc gtgcccgcgg tgcccagatc acagatattg
     3841 tcattatcgt tgtggctgct gatgacggag tgatgcccca gacacgcgag gcaatgaacc
     3901 atgcgcatgc agcaggcgta cccataatct ttgccataaa caagatagac aagcccacgg
     3961 ccaaccccga aaagattcgt gaggaactcg ccaacatgaa cttccttgtt gaggagtggg
     4021 gtggcaaata ccagtcacag gatatctcgg ccaagaacgg catcaatatc gatctgctcc
     4081 ttgagaaaat cctgctccag gctgaactgc ttgaactcaa ggctaatccg gataaacttg
     4141 ccaatggcac cgtgctggag tcgtcgcttg acaagggacg cggttttgtg gcaacggtgc
     4201 ttgtcaaggc aggcaccctg aatgtgggtg acgtggtcat agccgggagc tgcttcggga
     4261 atgtcaaggc catgtacaac gagcggaatc agaagatatc caaagccggc ccctcaatgc
     4321 cggtactgat acttggcctt aacggcgcac cccaggctgg tgacaggttc aatgtcatgg
     4381 agtctgaaaa ggaggcaaag gatattgcca ccaagagggc tcagctgcag agggagcagg
     4441 acctgcgtac ccagaagcat atcacccttg acgagatcgg gcgacgtatc gccatcggca
     4501 atttccagga actcaatatc attgtcaagg gcgacgtcga cggatcagtg gaggctctca
     4561 gcgattcact cataaaacta tcgacggaag agatccagat caatatcatc cacaaggctg
     4621 tggggcagat ttcagaatcg gatatcctgc ttgccgcggc atcgaatgcc attatcgtgg
     4681 gcttccaggt gagaccatca ctcagcgtga ggaagcttgc cgagaaggag ggaatagata
     4741 tacgcctcta ttccatcatc tacgatgcca tcgaggagat caaggccgcc atggaaggga
     4801 tgctctcccc ggagatcaag gaagaggtcg tggctaccgt ggaggttcgc gaaatcttca
     4861 agatcgggaa ggtgggaacc gttgccggct gcttcgtgcg tgacggcaag ataagccgca
     4921 acacgaaggt gagggtgata cgtgacggta ttgtaatcta taccggtgac ctcgggtcac
     4981 tgaaacggtt caaggatgac gtcagggaag tgacctcagg ctatgagtgc ggcctcaaca
     5041 ttgaaaactt caatgacatc aaggcaggag acgttgttga aggataccag accgtacaga
     5101 tcaaaaaaac cctttaggat ttgcagagac ctccggtgag aagcaggaga tgttgttaaa
     5161 gagaccgtac tggccaggaa aaccctccag gaaccggaaa ccgacacaca ataaaaaagc
     5221 tgcatcaccg cagcattatt ggcaaaacac aggaacggtt atctttcccg gatttttctt
     5281 tgtatgctct cttatgtagg agcgaagaaa gtgacagtga acaaaaaaaa aggtgccctg
     5341 ttgagacacc ctctttattt cacattgtat tgatctttaa gtctacaaga gcttcttgta
     5401 tccctcggca tcaagaagtt catcaatctc tgacggattt gaaagttcaa tcctgatcat
     5461 ccatcccttg ccataaggat cactgttcac aagctcggca gccgtgtcaa gctcctcgtt
     5521 cttctctttc acggtcccgc tgagaggcat aaagaggtct gacacagtct tgactgcctc
     5581 taccgttccg aatacttcgc cgtggtcaag ctcctcaccc acagtctcta tctcaacaaa
     5641 aacaatatca cccagctcat gctgggcaaa atcggtaatg cctacagttg cttcgtttcc
     5701 ttttataaga acccactcat gatccttggt gtacttcagg tttgtcggga gattcatttc
     5761 ttttatattt attatgttcg actttgatct tcaggtatca ggttgtcaaa aatacatctt
     5821 ttttattata aaacccggac aggggcattt tttcggtctc gccacggcta ttactgaaca
     5881 agggtgaatc tgaggctgaa tccgaagtta gtgtttgagg tcaggtaggt gtttgccaca
     5941 aacgggtcat tcattgtgtg gtcagcgtaa atctgcaggt tgaacctgtc actgagcaca
     6001 tagtcagccg tagcgcccag ggtgaagacc ctctgccctg ccaccggctg gttcacctct
     6061 tcaatcagct tgcgggcaag tgtcttgtta tccctgattg agagatcgag cttgatgtta
     6121 agatcactct gcagcgcccg ctgaccggtg cgtgaacgaa gtgtgatggc cacatcgtca
     6181 aaacggtaac cggcgcctat agtgatctcg tcattcctga tatcggcaac ctggttgctg
     6241 gtgaggttca gggtgacggt acgcgacttg cggtattcga tcctcgtggt cagtgagttt
     6301 ttccagccaa ggtcaatgtt aatgaacgga ctgaactgtt cattgatggt gacggtattt
     6361 atctcgtact gtggaatgaa gttcgacttc aggtctcgta tccggcttat tcccgactca
     6421 tcagggtcat agtaaaggct ggtgttgaag gagccgatgg tatatgttga cctgtactga
     6481 tgtgaaaggc tgaccgagtt gaatatcttt cttaccgcct cgaacctggt gagtccctcg
     6541 aagttgattc tccagttggg cataatgtgc agtatggagg ggaatggtga caatgatatc
     6601 ttgtcagggt ctctttttgt gtatgctgca aggaaagccg ggacgagcac ctcagccgat
     6661 gtgagtccgt aaccgctggc atacggacct gtgaccggct ctccggtgac agggtcgaat
     6721 ccggggtcat aactgttgtc aatgcgtgaa cgctcctgag cccttctctg tgagataatg
     6781 gcggtatact cgcggaaccg ctcgaatgtg cctgagttat agtcacctga cccgggaatc
     6841 ttttcaaagg cggtacccca tgataccact gagattgtga agtttccggt aatgcgtgtg
     6901 ttcctggtgc tgtcagggaa atttccgttg aggtctgccc ggagatatga tgagatggtc
     6961 tctgagtagc ggcggtcacc tgtgatgtct atccggaaac ccgggaacgg ctccagattc
     7021 gccctcatgt tgatctgttc gcgcttgctg aaggcagctg cattgttgag gagagtatcg
     7081 gtggtgatcc agccgttgat aactgcctca tcaaagaacc gctcgtctgt cagacccacc
     7141 agaaaccgcc agccgggaga tgacataccc ccgtagttct gctgcccaag gaaagtggtg
     7201 ccggggagat atccggggag aatgtgaccc tgttcaaccg tgtatgttgc cgagacattc
     7261 cgcaatgcca tcaatccacg taccaggtac ttgcccgcaa cgctggctat tccgggtttc
     7321 ttttccaccc tgcccttaat gtcaacactg gcatttgtca catcagcatc ggcagtgaag
     7381 tcgatgcgca catcagaaac tacatcaaat tttccgctga caactgtacc atccggtgca
     7441 ttgacggtga cagtcacatc cttcgttttg aggttatgaa taatgatgcg gggtttgcct
     7501 gccttgaagt tgagtcccct gcggctgtgg gtcacctctt catagccaag gctcatgcgg
     7561 cggacggcgc cgggttgcgt ggaagcctcc acctccttca ggaacttgac cttgctgtaa
     7621 aggtttgtaa ggttggcctg cagggtgaac tggctgttgt tggagttctt gatggtgttg
     7681 cccggattga tattcaggga atcggggaaa atggtaccgg ccagccagtc atacctggcg
     7741 ctgtagcgtg cgttggaagt cagccatgag agcagcggaa gcttgttgac aggaatgttg
     7801 tagttcaggt tcagcagatg gtaataactg gtggggcgcc cgaagctggc caggttggtc
     7861 attatggaat cacgccatgc gtcgaacagc tccggataac gcctgcggtc aacacctcct
     7921 tcaggctcat caatgcgggc tatgctgctg gcggtgaagt cgaccttcag ttgcttcgtg
     7981 atatcatact tgatatcgta gaaacggttc cactgaaaat cctttgtaaa caccggcttg
     8041 atgagcagat tgggattatt gatgttgcgt gtcctgatct cgttatagta cctgtacaga
     8101 tctgtacgaa cggtgatgtg cttcggaaag agatagatgt tgaaatcttt gatgagcctg
     8161 aagaccgaag agctgaacag ccccgatttc tggaacgggg tgaagttttt cggccgtgcc
     8221 tcaaaatcat aggcaatacc tcccctgtag gttcgctcca cgtcaatctc agtcctcgga
     8281 tcagtgttgt acacttcatt gaatgcatag cttacggtaa cattggccgg gtcccatgca
     8341 tgcggcttct cacctctcca tgtggtacct gcattgctta cggtgaatgt gcggcgtcgc
     8401 gaatactctt ctgacaggtt gagaacactg tcacgctctt ccttcgatcc gagattatca
     8461 agagcatcat tgagcaatat gtcaggatcg agcgggtcat actgtggtct gatgcggttt
     8521 tccgaatagc ccatgtaaac cggaattcgg atgcctgcct tctcgggaaa gaatttgcca
     8581 agctcaaggt tagaggagag atcatactgt atcacctgct ccatgcttcg ctggctgacg
     8641 ttcttgtcaa tgcttcccca tccgggtgtg cttgtctggc ccacgagatt aattgttccg
     8701 aggtcagcca gtctggcctg catctgcgca ttggcagccc agccgccatc ctcgcggaaa
     8761 tcggcaaggc gaagttcatt gacccatatc tctgccgata gcggggcacc gtcatcagtt
     8821 tgcgggcgac gtgtgcggat ggggttcctc acgccaacca tcacaaccct tacattactg
     8881 aggttgggat taccgcttac cgagatgcgg gctattccgt catgcactga ccagatgtca
     8941 gcctcgctca ctactgaacc cggagcacgc atggccgcat cacgtgactg tttggcgtca
     9001 agcaacagag aaagatcaat atctatcttg ttcagctcgg gccagacaac agccctctgc
     9061 tcctcgcttt cattgctgta cctgccatac ggagtgagct tcaccggtat ctcgtactca
     9121 tagaagttat tcttgtagtc agaccccagc cttacaaaca ctgtcagctc gtcatcctga
     9181 agcggttcac cgattagcgc ctcggcatgc acctccatct tcagcctgcg gtattgcctc
     9241 atgtcaaggg ccacattctt gtaggtggcc ctggcatcac cgtcggccag atcgcggaca
     9301 cgaagcacca tagactgctc attaagctgc ctcaggtgag gatttgacgg gtcaatgacc
     9361 cggtcaaagc ccggaggcag gacgtagttc accggctcct tgccggcatt ttcctcaata
     9421 cttaccgagc tgatctcaaa agaaccgtca tcctcttcgg gaacagtgat gcgctcacca
     9481 ccctcaaaga aggagaggtt gtatttgcgc cattctgacc tgataagctc cagccgtgca
     9541 aaacgcatga taacatgctc atcgaaatta cgcatgtaca tccggaggaa cctgattgac
     9601 ttgaaatcgc gtatcgagcc tacgatcctt tcatagtcgg tgatcggtat cttgaactgg
     9661 taccatttga cgggcgattt ttcgccgttg gcgaaagtgg ctgtgtattc aagctggtca
     9721 acgatgaagt tcctgcccac aaccatatcc tcaggcctca ggcttacgtg gtactgataa
     9781 tagctctccg tctcgctgag ggtgttgtca cggttgatat cctccgtgtc aggcagcgtg
     9841 gaaccgctgg tgggatatga ctcgtctgac atctccgatg tgggtgagtt gccttcggtg
     9901 ccgttgaaat tcttgtagcg ttcaaggatg ccaagctgca gcaggtcata gtcagacccc
     9961 ctgaaatagt ggaagtcatc attcgccgga tcttgaagta tcttttcaag aatctcgggg
    10021 ctcacaaccg ccccgacagc ctgcaggtaa ctgtcaaaga aggttctctc atcctcgttg
    10081 ctaaggccgt caaggccaac atcctggtag cgacgtgact caatgttgtt gtcaaaggcg
    10141 tgtaccacgg cctgaaccgt aggaaccctt ccccatacag tggtgtcaac attcaccacc
    10201 agcggcgagg tgggcagccc gttttcaaac tgcttccgcg aatcgcgaag aatatcctcg
    10261 gaaatattac ccaggttgaa gtaaagatca ccaccttcat ggtcaggatt ctcaacgaac
    10321 ggatccatca gccagaactt gatgtactgg atgtttgccg actcgaagtc atttgtcagc
    10381 agctcgcgca tcatccctcc ccagcgggtg cgcggcgagt tcagaagtcc gtcgtaattg
    10441 agccctgccg aatattctcc ggcctgcgca tcaaagttgt acggcccgcg ctcctcagga
    10501 tagaatgcca cgttcagcac tgagatgttt gtagggatac cgctgggcga ctccttgtaa
    10561 gggaaaatct cggtctcata gatctccctc acaaagtggc tcgactgggt gtcaggattg
    10621 gagcggatat gccccggggt ggttgaaccg ttgcgcagga agagcggatc gatgacatac
    10681 catgccagcc ttgccctgtt gattcctgaa cggagatcct tgctcagcct tgcctcaggg
    10741 aaataatgat caaggccctg gggtatgctg gccagtgacc atgcattgaa cgccctgagg
    10801 tccagagaaa tctcgcttgc ttcaaagtca tcaatgtaaa cagctccctc cgatgtgatt
    10861 gccctgctgt gcccgggtac cagatgtgca aattcgccaa agaagtttat agatgaagga
    10921 gtgttcgttt ttatgaacgg gagcttgttg atgacattgg tcaaaagctg tgactctgag
    10981 cggtatgatg catcaaaacc atagatggtg ttggagatcg ggtcatcacc atatgtaact
    11041 ttctgtgtat aaggcctctc agtaagtctc agcaccgtgc cgccaagatt gaacttgtca
    11101 gagaagcggt agttgaggtg ggtcccgaga agcgtttttg tctgcaggcc gaagaactgg
    11161 ttgctttcaa gtgatacctg taccggtgtc tgagactcga ttattgctga gttgataatc
    11221 tttaccgaac ccatgttgta gtcaaccgtg tagtcaacgt tctcagtcag cgtaactcca
    11281 cctgcggtaa cctttactga cccctccggg atatttgcca cgtttagtct tatctcggag
    11341 cccgatgcgg atgtgaactg gcctctcagg aggtacttgt tcttctcggc catctgcctt
    11401 gccaccgtct gggtggagtc atacagttca gtaaagacat attttctgat ctgctccgga
    11461 tcagtgatat acttcagcag gtggcggcca aacggttcaa gcaccgggaa aataatcctc
    11521 cccctttcag ccatcaccgt tatgccgtca acgaagtcaa acaggccgtc aggctgccgg
    11581 tcaagctgtg agttgagctg gtcaagcccg agagcctgaa gcaatacctg tccatcaagc
    11641 ggggtgccgg gcaggtagtt cagggcattg cccgtcttgt catcctggta gagtatgtta
    11701 acctcgaaat tctttggttc aatcctccct gagccgatgt tgtagatgtt tttcatcatc
    11761 agcttccagg tgggtatctg cggactcagg gatgtgcctt ttatgagctt gagaatgagg
    11821 gcgtcaggcg cagtaatacc gtctgtagag aactcgccca ccttgaatac ctgcccgttc
    11881 agggtgtact cgtaagctac cgcaagaacc tcatcattat taagggccac gttcagggag
    11941 atatagccca gctgccggtt caccgtatat tcgcgttccg tcagtttgcg tgcattctct
    12001 atcttctcgt agtcacgccc tatctgaaat ccggggtata acccggcaaa agcatcagcc
    12061 acctggtcaa tgttccttac attgctgtat gtggtgttga gctgttcata gagaccgttg
    12121 gtgttgttgg agggattcag cggtgtgccg ggaattccct gaaactcagg aacactattg
    12181 aatatatttg tctggttctc tgcaaggtca aggaaggcaa taatgttcct gttgctgaca
    12241 tcctcaaaac ggcttgtctg gttggtaatc cagacctcga tcttctcaat attgagacct
    12301 gtgctgacta tgggcaggtt cgagagcgcc ccgtcaaaat tattcctgaa ctcctgcgag
    12361 aggaaaaagt gacggttggc ttcgtaattg tcagcagtaa tctcgtagtc acttatctgt
    12421 gaacctcctt tcacctccac cactgacgac tcacccttct gctgtgagaa gacggttgtc
    12481 atcgtcagct ttccgaactg aagctcggtc ttcaggccga agaggctgta gcttccggtg
    12541 atcagggtac cgttgagcgg aagggttaca tcaccggcct ctatcttctt gatgatctca
    12601 tcctccttgc cggagtattc aagcttggtc cggttttcaa actcgaacat tgcttccgta
    12661 ttgtaattga tgcccagttg catcttgtcg ccgatggtgc cggtgacatt catctgtatc
    12721 ttttcctgga agtcaaatgt aggaatggtt ctcagccttt ctgagatggt agggttctca
    12781 gtatgtgaaa tattgatgcc gaatatcagt tctgccgatc cctgcgggac gatatttatt
    12841 atgttgctgc cgaagatgcg gtcaaaagtc tcccctccga cctctatctg cgggatgagt
    12901 gacgacctgt agccggttgc gtcaccccgc aggctggcgt cccagtactc tctcatggcc
    12961 tggttgaatt cgaacctgcg gtactcttcc gctgacatgt ataccggttt gcggaagtcg
    13021 agagtgcctg ccttctggta tatgatgtac tggttgttcc ggccgtcata ctctacccgg
    13081 gtggtaatgt tggaggggtt gtagaggaag agaggtgatg actttttagc ctcccacggg
    13141 aatgccggat catagttcag tctgaggaga gaattgtcag tctcggtctg cgcctttatg
    13201 acaagcgaag aaagggagaa gagcatcaat agccccaccc tgatcaggag atctctatta
    13261 tgtaaagaaa actgcaatcc ggcaccctgt ttataacagt ttcagcgcct tcctgactat
    13321 ctcttccacc tggagtttat tgttttccct gaggatgtta tcaataacct ttaagacgct
    13381 gctcctggca aatccaagca tcactaaagc agataacgct tcttcacgtg cagtattgtc
    13441 ggaaaacgta agaatttcgc cggcgccggc atgttttccg attttgtctt tcaggtcaac
    13501 tattatcctc tgtgcggtct tcagacctat tcccctgacg cccttgagaa tggccacatt
    13561 gccactgatt attgcctccc tcaggtcaga aggagtcaac gaagaaagca tcattctcgc
    13621 tgttcccgct cccactccgc tgacagttat gagctgtctg aacaggttgc gctcatctgc
    13681 agtagcaaaa ccgtacagga gatgggcatc atcgcggata gcctcatgaa tcagtatttt
    13741 gcatgaggca tccttttcga gggcagaaaa agtattcagg gaaatattta tatcatagcc
    13801 tatgccgttg cactcaacca ctgcacaggc aggattgagc tcggtcacgg tgccggtaat
    13861 ataatcgatc ataatgcctg gtcgtaattt tcggtctcgg gaggaagctc atcaacgtgt
    13921 tcgggataaa tctcaatttt ttgctgctgc agcggattgc ggtttatctc ttcatgcatc
    13981 tccctgttac ggaatatgtc cactgca
//
LOCUS       DAOPBK010000059        13964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4390_length_13964_cov_5.445179, whole genome shotgun sequence.
ACCESSION   DAOPBK010000059 DAOPBK010000000
VERSION     DAOPBK010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4390_length_13964_cov_5.445179"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLA17_04925"
     CDS             <1..276
                     /locus_tag="PLA17_04925"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPK84791.1"
                     /translation="EQPVITSPDTVTAGEALILNADDTYLPGIKIDRYYWNFGDETVA
                     TGLEVSKIFTIPGRYNIQLIVSSAPDARGVVREFCVCKDIEVSANVK"
     gene            293..1279
                     /locus_tag="PLA17_04930"
     CDS             293..1279
                     /locus_tag="PLA17_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84792.1"
                     /translation="MRVNRLRLSLLALVVMAAPVVLRAQEVPGPDENIPFLITFGRDG
                     STSWGDDDFSQTFFFALPKDYKDPFYIRVYDPDVGGKTDELNNFWDTRMVYSIYGGKG
                     CYTDPDANGIDPKGNYKSGTLLATRTFGMDNRYDEQWFTFGPFNPADGEYYPKFLSNI
                     FKIICDGVSGDDGNLYKYALSRRPDANVPIDGANAFTYEYTFRLWNNNDTSFVSHIYP
                     FIDSGCIWVQQRNFDWDNDGHAMVVSVERKGQMIPISGENEWATSKIQIYEGEIGKSL
                     DFRFYKRRDELVKNNNVVVSVQNQYGENLPFFSAPIGGVPVYKPRIKVVPKK"
     gene            1282..4440
                     /locus_tag="PLA17_04935"
     CDS             1282..4440
                     /locus_tag="PLA17_04935"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPK84793.1"
                     /translation="MSGRFRYSIAVICIVCVNVLHPFGSSAQTYSFRTYDEYTGLPGT
                     WLTVIAQDRSGLLWMGVDTGLYRYDGFSFHHMPFPDTLPRGNVSALFCDRKGTMWVGM
                     NDGSLFTWNMTGGLKRMMLEDSDRINRITGSPDGKVWIVTQTSGIIIAAPGGDEKITR
                     MMLPDDMTLFDIAFAGNGTFLAATQNNLHLCAAEGDKAVSRLTFPELDYTWIQTVVRM
                     DDDTWFAGTDNMGLWMISRSGDSINARSVGDTLFMNTNIPALNAGPERTLTIATKELG
                     AVKVKFSADFSEMFITGRYDVTSGLPDNNVRTIFTDREENLWIGLYTSGLAAVTTNAF
                     TFCRPESDKEITYIGRAGTQVVAGTRSGLFDFDPLTGNFTRYRNIAARTGGSGITSWS
                     ADDPGNIWIGTAGDGLWLMKPDGNLKQFYRSGSPGRNHINSVAVENNNVWLGTSDGVV
                     LLDRETGVQKSVYTTMELLPHNFISQVVVNRDGEVLVATKSDRLCYIHVTGGVRTEGE
                     LMEGRLVNDIISISVAHDRSVCAGTKGNGLFLFAADTLLNYTEDDGLLNNFCYSAMIA
                     SDGRIWTGHQKGFSIINPATGSVRSYSTDFGVRGDCLSNALAQLPDGTVCVGTTDGLV
                     VYNPSLEPDSIIAPQAEIISVVINNVVYPYRESYNLPYRKSYTVKVNYAGIFLRDPLN
                     VVYRTRLDKDEQWGEITGDRSVAYSGLRDGHYQFSVEALTRDDPESATIASFDLVIQK
                     PVTDRWWFLLSVLALIAGVVYIIVRLRERAHRKQREYLESELQKRTAEVNEQKEELAQ
                     KNLDITESIKYAKRIQSSVLPDIARLNTVFREAFVFFQPRDIVSGDFYWFDWVDKDRF
                     IVVCSDSTGHGVPGAFMSMIGTALLQDIITVKKIIRPSQILHELDRQIFSTLNQNQEL
                     EASNDGMDIVVCEFNMATRQLVFSSAMRPVILVIDGVQHYVRGNRSSVGGESVSEKFY
                     DDQEYHLREGDMVFLFTDGYCDQFGGPGGKKMKISRLKTLIDDISPMSAADQYREVKG
                     FFFEWMGEGDQVDDVLFMGMRV"
     gene            complement(4441..4962)
                     /locus_tag="PLA17_04940"
     CDS             complement(4441..4962)
                     /locus_tag="PLA17_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84794.1"
                     /translation="MKNAIIITVIMVGLMMASCWSVETLPPEPAIEFRAFTIYDTTDI
                     LGNPAKAGKITFYFEDGDGDLGLNPPIEPGVDPSSNLFFRLYRKVNGTFEPAAPSDPL
                     YPSEYRIPYIDADGQNKIIKGTIDVTIFYLFYNPDDTIYYDFWIKDRSGNESNTATTC
                     IVVLGENGTCLPE"
     gene            complement(5007..6311)
                     /gene="der"
                     /locus_tag="PLA17_04945"
     CDS             complement(5007..6311)
                     /gene="der"
                     /locus_tag="PLA17_04945"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPK84795.1"
                     /translation="MSKIAAIVGRPNVGKSTLFNRLTESRKAIVNEESGVTRDRHYGK
                     ATWNGVDFSVVDTGGFVENSDDVFESEINRQVKLAISEADVILFMVDVTTGIHELDKA
                     VAAMLRRVTKKVILVVNKVDNNKRLLEAAEFYGLGLGDYFPVSSVNGSGTGDLLDELV
                     KHLPETPEEEGEDIPKVAVVGRPNVGKSSLANTLLGEERNIVTPVAGTTRDSVHSLYN
                     KFNHRFLLIDTAGLRKKAKVDEDIEFYSILRSVRAIEESDLCLLLTDATRGFEAQDMS
                     ILGIIQKNNKGVIVLVNKWDLKGKDHTTAKQTEEEIRKKTAPFIDYPIVFISAITKQR
                     IHKVPELISNVYENRSRKISTSKLNEVMLDIIGKTPPPSTKGKHITIKYVTQLPAEYP
                     VFAFFCNLPQYIREPYKRFIENKLRENFRLTGVPVRIFFRKK"
     gene            complement(6324..7202)
                     /gene="era"
                     /locus_tag="PLA17_04950"
     CDS             complement(6324..7202)
                     /gene="era"
                     /locus_tag="PLA17_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPK84796.1"
                     /translation="MEHRSGFVNILGNPNVGKSTLMNVLVGHKLSVTTPKAQTTRHRI
                     MGIVNGDGYQIVYSDTPGILNPKYKLQSAMMRSVESALDDADLILYVTDVVETASKHS
                     EHLNRILASPVQKIIVINKIDLTNQSDLEKLVEEWRERAPGVPVLPVSAVNRFNTDLL
                     LKEIISRLPEGPPYFPEDQLTDRYDRFFVTEIIRGKIFETCHKEIPYSVEVEIESYRE
                     EPEINRIAAVINVARDSQKGIIIGHKGSMLKRIGTAARKEMEEFLGKKVFLELFVRVA
                     HEWRDNPRMLKKFGYL"
     gene            complement(7212..7976)
                     /locus_tag="PLA17_04955"
                     /pseudo
     CDS             complement(7212..7976)
                     /locus_tag="PLA17_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            8093..9472
                     /gene="glmM"
                     /locus_tag="PLA17_04960"
     CDS             8093..9472
                     /gene="glmM"
                     /locus_tag="PLA17_04960"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPK84797.1"
                     /translation="MALIKSISGIRGTIGGKPGDSLTPVDIVKFASAYGTWLRRRGRG
                     PLRVVTGRDARVSGPMVAALVNSSLRSVGIDVIDTGMASTPTVEVAVTGLGAGGGIIL
                     TASHNPAQWNALKLLNEEGEFLSDADGREVLALAEAEDFDYVTYDRLGTETVDDSWTE
                     KHIRMVTGMTLTDCDAIRKAGFTVAVDAVNSVGGIVIPELLRALGVKKVIELYCEPDG
                     LFPHNPEPLPENLSEISRTVKESGAHVGFVVDPDVDRLAIVCEDGSMFGEEYTLVSVS
                     DYVLGHVKGPTVSNMSSTRALRDVTESHGCSYTSAPVGEVNVVAEMKRTGAVIGGEGN
                     GGVIYPALHYGRDALVGIALFLTHLAKQGVRCSELRKRYPDYHIAKTKIDLPGSIDMK
                     ELREKLVMKYPEADCDTRDGIKLDLPAGWVQIRKSNTEPIMRIYAEERTMEQAEKLAA
                     DVTAIVKNL"
     gene            9731..11245
                     /locus_tag="PLA17_04965"
     CDS             9731..11245
                     /locus_tag="PLA17_04965"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK84798.1"
                     /translation="MMKKTIIITSIIVLLAIIAMIIISKLSGRKDLSILFTEVQYGDF
                     EVVVTTTGELQAEVSTDIKGPEGLSSRNMRFGGIKISDLVPEGTEVKAGDYVATLDRS
                     SVDNSLKDELDRLESLETDLLKKQLDTTISLGNLRDELLNLKFNMDEAEITLEQSQYE
                     PPTTIRQARISLDKAQRSYDQAVSNYDLKVRQARADIRDIELQVSKQRRKVAEMQDII
                     QKFVISAPADGMVIYKREWGGAKRKVGSEISAWDPVVATLPDLTSMISKTYVNEIDVS
                     KVKAGQRVRLTVDAFPEKSYTGVVLSVANIGEQLPNTDAKVFEVISKIDGSDPILRPS
                     MTTGNQIITKTFSDVLFIPLETVFATIDSIPFVYKKDGTRQVVVLGESNENDVIVEQG
                     LKEGERLYLTIPEDGEKFKLQGDDLIAIIKERKKQEAEEEAKRLQNGNQPQNMMPENM
                     TPEKMREFMQNLTPEQREAMRQMRGSGRPGQMPGRPAGGSDTTVRMQRERTPNQ"
     gene            11286..12656
                     /locus_tag="PLA17_04970"
     CDS             11286..12656
                     /locus_tag="PLA17_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK84799.1"
                     /translation="MFKRYGHDIRIALESILSNKLKSALTALGIIFGVAAVISMLAIG
                     KGAQQEILDQIKMVGVNNILISPIVQEKDAAEEDGEKQLNKFSRGLTLLDVEAIRSTI
                     PAVKRISPEISFNSTIVLSGMRHTAKLVGVSNDYFELYNLPLSQGAFFNDYQEEYGIQ
                     VCIIGANIRAKFFSQVDPIGQYLKFDNVWLKVMGVLEKTNVSLTGFEEQGVNVYNDNV
                     YIPIKTMLMRYQNRALVNTKLASEASVHVFGGGRGGRGRVVVSSTNASTANAETNYNQ
                     LDKIVVQVAETEQLTPTTEVLSRMMLRRHSGVKDFEITVPELLLKQQQRTKDIFNIVL
                     GAIASISLIVGGIGIMNIMFASVMERIKEIGTRMAIGAKKMDIVVQFLSEAVLISVSG
                     GFIGIFLGIILAVLIQKIAGITTIVSFFSVVVAFGVSAAVGVIFGYSPAKRASERDPI
                     ESLRYE"
     gene            12673..>13964
                     /locus_tag="PLA17_04975"
     CDS             12673..>13964
                     /locus_tag="PLA17_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK84800.1"
                     /translation="MKSIKALSLTVLFSALVALPAMPQEVRLLTLDEVIKLAADQSPN
                     AYIAKHRFRASYWQYRTFVAEYRPTLSLSGNMPDYSTAYSRVWNSVAQQWEYASTNVL
                     QTSGNLSLAQNIGLTGGSVSLFSDLNYEKNFETGGERYITSPLNVRLTQPLFRYNELR
                     WQKKIEPLRYEEARKTYLRDVENVHMVAVQYFFNLALAQINKEIAETNMQNADTLYQI
                     AKGRYNLGTIAEDELLQMELSYLNAGTAINESEMNLRDRELKLRSFLGFNQSVRLELI
                     IPSEIPDLEVDVSEVLRLAEENNPELIALERQLVEAQSSVAQARAEKGLNANLTASYG
                     LRDQDPLLQQAYTHPNQQQTIRVSFSLPILDWGQGRGRYKMAQSSEELTRVQVEQSRI
                     DFEQNLLLDVQQFNMQDDQVIIAAKSDTVAAKMFEVTK"
BASE COUNT         3541 a         3291 c         3653 g         3479 t
ORIGIN      
        1 gagcagcccg ttatcaccag cccggatact gtcacagccg gagaagcgtt gatccttaat
       61 gcagatgaca cttacctgcc agggataaag atagaccgct actactggaa tttcggggat
      121 gagactgttg caaccggact ggaagtatcg aagatattta ccattccggg aagatataat
      181 attcagctga tagtcagttc agcacctgac gccaggggag ttgtgcgtga gttctgtgtc
      241 tgcaaggata ttgaagtatc agcgaatgta aaatgattat tgaaggagaa agatgagagt
      301 aaatcgctta agactgtcgc ttttagcact tgttgttatg gctgcccctg tggtgctgag
      361 agcccaggaa gtaccgggcc ctgacgagaa cattccgttc ctgataacct ttggcaggga
      421 tggaagcact tcatggggag atgatgactt ttcacagaca ttcttctttg ccctgccgaa
      481 agattacaag gatccgttct acataagggt gtatgacccc gatgttggcg ggaagacaga
      541 tgaactcaac aatttctggg atacccggat ggtctactca atttatggtg ggaagggctg
      601 ctacactgat ccggatgcaa acggaattga cccgaaaggc aattacaaga gtggaacgct
      661 gctggcaacc cgtacattcg ggatggacaa caggtatgac gaacagtggt ttaccttcgg
      721 acctttcaac ccggctgacg gtgagtatta ccccaagttc ctgagcaata tcttcaagat
      781 catctgtgac ggtgtctcag gtgatgacgg caacctttac aagtatgccc tctcgaggag
      841 acctgacgcc aacgtgccta ttgacggcgc aaatgccttt acctatgaat acactttcag
      901 gttgtggaac aacaatgaca catcatttgt ctcccatatc tatccgttta ttgacagtgg
      961 ctgtatatgg gtacagcaga gaaatttcga ctgggacaat gacggccatg ccatggtggt
     1021 atccgttgaa agaaaggggc agatgatccc gatttcaggc gaaaatgaat gggcaacatc
     1081 aaaaatacaa atctatgagg gagagatagg aaagtcactt gacttccgct tttacaagag
     1141 acgtgacgag ctggttaaaa acaacaatgt ggtggtatct gttcagaacc agtatggaga
     1201 gaatctcccg ttcttcagcg caccgatcgg aggagttccc gtatataaac cacgtataaa
     1261 ggtagtaccc aaaaagtaat catgtcagga agattcagat attcaattgc agtaatatgt
     1321 attgtttgtg taaatgtgct gcatccgttt ggttcatctg cacagacgta cagtttccgt
     1381 acctatgatg agtataccgg cctgccgggt acctggctga ctgtgatagc gcaggacaga
     1441 agcggccttc tctggatggg agttgacact ggcctctacc gttatgacgg cttctcattt
     1501 catcatatgc catttcctga taccctgccg cggggtaacg tgagcgccct cttctgtgac
     1561 cggaagggaa cgatgtgggt gggaatgaat gatggctcgc tcttcacctg gaacatgaca
     1621 ggaggattga agagaatgat gctggaggat agtgacagaa tcaaccggat aacaggcagc
     1681 cctgacggca aggtttggat agtcacgcag accagcggga ttatcattgc tgcccccggt
     1741 ggtgatgaaa aaatcaccag gatgatgttg cccgatgata tgactctgtt tgacatcgcc
     1801 tttgccggta acggaacatt tcttgctgca acgcaaaata atctgcatct ctgtgctgct
     1861 gagggtgata aggcggtcag caggctcacc ttccccgaac ttgactatac atggattcag
     1921 actgttgtcc ggatggatga tgacacctgg ttcgccggta ctgacaacat gggcctgtgg
     1981 atgatcagca ggagcggtga ctcaataaat gccaggagtg tgggtgacac cctcttcatg
     2041 aacacgaaca tcccggcact caatgccggg ccggaaagga cgctgaccat tgccacaaag
     2101 gagttggggg cagtaaaggt taagttcagc gctgacttca gtgagatgtt catcaccggg
     2161 agatatgatg tcacttcagg tctccctgac aacaacgttc gcaccatttt taccgaccgg
     2221 gaagaaaacc tttggatagg gctatatacc agtggcctgg ctgcagtcac aaccaacgcc
     2281 tttaccttct gcaggccgga gagtgacaag gaaatcacct atattggccg ggccggcaca
     2341 caggtggttg ccggaaccag gagcgggctt tttgacttcg atccgcttac cggaaacttt
     2401 acaaggtacc gtaatattgc tgcaagaacg ggcgggtcag gcattacctc ttggtctgcc
     2461 gatgaccccg gaaatatatg gataggaaca gcgggagacg gtttgtggct gatgaagccc
     2521 gacgggaacc tgaaacagtt ctatcgttcc ggcagcccgg gacgcaatca tatcaattcc
     2581 gtagccgtgg agaataataa tgtctggctc ggaaccagtg acggagtagt gctgcttgac
     2641 agggagacgg gagtgcagaa aagtgtatat acaacgatgg aattgctgcc gcacaacttt
     2701 atctcacagg ttgttgtcaa ccgcgacgga gaagtgctgg tggccacaaa gagtgacaga
     2761 ttgtgctaca tacatgtgac cggaggggta cggactgagg gcgagcttat ggagggcagg
     2821 ctggttaacg atattatcag tatctctgtt gctcatgacc gttctgtatg tgccggaaca
     2881 aaaggcaacg ggctctttct ctttgctgct gacacacttc tgaattatac cgaggatgac
     2941 gggttgctga acaatttctg ctacagcgca atgattgcct ctgacggaag gatatggacc
     3001 ggacaccaga agggattttc aatcatcaat ccggcaaccg gctccgtaag gtcctatagc
     3061 accgacttcg gggtgcgggg cgattgtctc tccaatgcgc ttgcccagct ccctgacgga
     3121 accgtatgcg tcggaacaac agacgggttg gtggtgtaca acccttcact cgagcctgac
     3181 tcgataatcg caccacaggc agaaatcatc tctgtggtta tcaacaatgt cgtttatccc
     3241 tacagggaga gttacaacct cccatacagg aagtcataca ctgtgaaggt aaattatgcc
     3301 ggtatctttc ttcgtgaccc cctgaacgtg gtctaccgta ccaggcttga caaagatgaa
     3361 cagtggggtg aaataaccgg tgacaggtca gttgcctaca gtggtctgcg tgacgggcac
     3421 taccagttca gtgttgaagc cctgaccagg gatgaccccg aaagtgcaac cattgcatca
     3481 tttgaccttg taatacaaaa acctgttact gacagatggt ggttcttgct ctcggtgctg
     3541 gcactcattg ccggtgtggt ttacattatt gtcagacttc gtgagagggc ccacaggaaa
     3601 cagcgcgaat acctcgagag cgagttgcag aaacgtacgg ccgaagttaa cgagcagaaa
     3661 gaggaacttg cccagaaaaa ccttgacatc actgaaagca tcaagtatgc caagcgtatc
     3721 cagagcagcg tgctccccga cattgcccgc ctcaacactg tcttcaggga ggctttcgtc
     3781 ttcttccagc cgcgtgatat cgtcagcggt gacttctact ggtttgactg ggtagacaag
     3841 gaccgtttca ttgttgtctg ctctgactcc accggacacg gggtgccggg tgcattcatg
     3901 tcaatgatcg gtacggcgct gctgcaggat atcattaccg tcaaaaagat aatcagaccc
     3961 tcacagattc tgcatgagct tgacaggcag atattctcca ccctgaacca gaatcaggaa
     4021 ctggaagcat caaatgacgg tatggacatt gtggtatgcg aattcaatat ggccaccagg
     4081 caactggtat tctcatcagc tatgagaccc gtaatattgg tgattgacgg agtgcagcac
     4141 tatgtaaggg gaaacagatc atcagtaggc ggtgaatcgg ttagcgaaaa attctatgat
     4201 gaccaggagt accacctcag ggagggagat atggtcttcc tcttcactga cggttactgt
     4261 gaccagttcg ggggaccagg gggcaagaag atgaagatca gccgtctgaa gactctgata
     4321 gatgacatca gcccgatgag cgctgctgat cagtacaggg aggttaaggg cttcttcttt
     4381 gaatggatgg gtgagggtga tcaggttgat gacgtcctct ttatggggat gagagtctga
     4441 tcattcagga aggcaggttc cgttttcacc cagcacaact atgcatgtgg ttgcggtgtt
     4501 gctctcattg ccgctcctgt ctttaatcca gaaatcgtaa tagattgtgt catccggatt
     4561 gtaaaacaga tagaagatag taacatctat tgtacccttg attatcttgt tctgcccgtc
     4621 cgcatcaatg tatggtatgc ggtattctga agggtaaagc gggtctgaag gtgcggcagg
     4681 ctcaaaagtg ccgttaactt tgcggtacag cctgaaaaag agattgctcg acgggtcaac
     4741 gcccggttct atgggcggat tgagcccaag gtcaccgtcg ccatcctcga agtaaaatgt
     4801 aatcttacct gcttttgcag gattgccaag tatgtctgta gtatcataga tagtgaacgc
     4861 acggaactca atagcgggct ccggtggcag cgtctcaacg gaccagcagg aagccatcat
     4921 caggccaacc atgataactg ttatgataat tgcattcttc atatgcagtc tatacatccc
     4981 cgcatcaaaa atcaaaccaa atgtatctat ttttttctga aaaaaatcct gaccggtact
     5041 ccggtaagcc tgaagttctc ccttagcttg ttctcgatga accttttgta aggctccctg
     5101 atgtactggg gcaggttgca gaaaaaggca aataccggat actctgccgg gagctgggtc
     5161 acatacttta tagtgatatg ctttcccttt gttgaaggcg gaggagtttt accgataata
     5221 tcaagcatca cctcattcaa cttcgaagtg gaaatcttcc tgctcctgtt ttcatatacg
     5281 ttcgatatca gttcaggcac cttgtgaatc ctctgcttcg tgattgccga gatgaaaact
     5341 atagggtaat caataaaagg tgctgtcttt ttcctgatct cttcttctgt ctgctttgcc
     5401 gtggtatgat cctttccttt caggtcccac ttgttcacca ggacgatcac tcccttgttg
     5461 tttttctgaa tgatgccgag gatgctcata tcctgagcct caaagccgcg ggtggcatct
     5521 gtcagaagca ggcagaggtc gctctcctcg atggctctta ccgatctcag tattgagtag
     5581 aactcgatat cctcatccac ctttgctttt tttcgcaggc cggcagtatc tatcagcagg
     5641 aaacggtggt tgaacttatt gtagagactg tgtactgagt cacgtgtcgt gcccgccaca
     5701 ggggttacaa tgtttctctc ctcacccaga agggtgttgg cgagtgatga cttgccgacg
     5761 ttcgggcggc ctaccacagc cacctttggt atatcctccc cctcctcttc gggtgtctcc
     5821 ggaaggtgtt tcaccagctc gtcgagcaga tctccggtac cgcttccgtt gaccgagctc
     5881 accgggaagt agtctcccag tcccagaccg taaaactctg ccgcttcaag aaggcgtttg
     5941 ttgttatcaa ccttgttgac cacgaggatc accttcttcg taaccctcct cagcattgcc
     6001 gccactgcct tgtcaagctc atggatgcct gttgtcacgt caaccatgaa gagtatcacg
     6061 tcagcctcgc tgatggcaag ctttacctgc ctgtttatct cactctcgaa gacatcgtcg
     6121 gagttttcca cgaagcctcc cgtgtcaacc actgaaaagt ccacaccgtt ccaggtggct
     6181 tttccgtagt gcctgtcgcg ggtgacgccg ctctcctcat tcactatggc cttgcggctc
     6241 tccgtaagac ggttgaagag ggttgacttg cctacgttgg gtcttccgac aattgctgct
     6301 atcttgctca taaacactaa aatctacaga tatccgaatt ttttcagcat acggggattg
     6361 tcacgccatt catgagcaac ccttacaaac agttcaagga agactttttt cccgaggaac
     6421 tcttccatct cctttctggc agccgtgcct atacgcttga gcattgagcc tttgtggccg
     6481 atgattatcc ccttctggct gtcgcgggca acattgatga ctgcggctat ccggttaatc
     6541 tctggctctt cacggtaact ctcaatctcc acttccactg aatagggaat ctccttatgg
     6601 caggtctcga aaatctttcc cctgatgatt tcggtgacaa agaaacgatc ataacgatca
     6661 gtgagctgat cttcagggaa gtatggaggg ccttcaggga ggcggctgat aatctccttc
     6721 agcaaaaggt cagtgttgaa tctgttgacg gctgacacgg gcagcaccgg cacgccggga
     6781 gctctctctc tccactcctc cacgagcttt tccagatcag actggtttgt gaggtcaatt
     6841 ttgttgatta ctatgatctt ttggacgggg gaggcaagta tacggttgag gtgctcactg
     6901 tgctttgatg cagtctcaac cacatctgtc acatagagaa tcaggtctgc gtcatcgagg
     6961 gctgattcca ccgatctcat catggctgac tgcagcttgt atttcgggtt gaggataccg
     7021 ggagtgtcag agtagacaat ctggtacccg tcgccgttca cgatacccat aatcctgtgg
     7081 cgtgtggtct gcgccttagg ggtggtcacc gagagcttat gaccgacgag tacgttcatc
     7141 agtgtcgatt tccctacatt gggattgcca agaatattta caaaacctga tctgtgttcc
     7201 atgacgggca gttatttacg gatgccgcgc ttgagcatgc tgacttcctt ttgcgtaagg
     7261 tgcctccagt ggcctcgttg caggttcttt tttgtcagac ctgcaaaact gacgcggtca
     7321 agttttctga ccttataccc aagtttttca aagatgcgtc ttaccaccct gttctgccct
     7381 gagtgaatct caatgcccac ctgcgtgtgg tcttcagagt cggcatacga tatggcatct
     7441 gcgctgatct ttccctcttc caggtcaact ccgctgacga ttgtttccat gtcattcctg
     7501 gtgacctcac ggtcaaggaa gacatgatat acttttcccg tgccgtatga agggtgtgtc
     7561 agctttccgg ccagatcacc gtcattggtc agcagcagta cgcctgtcgt tgccttgtca
     7621 agccttccta cagggtatat ccgttctgtg gtggcatcct ttacaaggtc aaggacggtg
     7681 ctttcggcat gaggatccct gacggttgtc acataaccct ttggcttatt gagcagtata
     7741 taaaccttct tgcctggtgt cagcaggcgg cccctgtatt ccactttatc ggatggcatt
     7801 acctttattc ccatatcggt cactttcttc ccgttgaccg atatataccc ctgttttatc
     7861 agctcatcgg cttcgcgcct tgcgcagact ccgctggctg caatgtattt gttgagcctg
     7921 acaggttcgg cctccggttt cagggtatct ttgccttttt taaacttttt ccgtagcccg
     7981 gctctctttt tttccatcct ctgactttta cagagtgcaa atttagaact atttaccggt
     8041 ttttatcccc gttatttttt gcacctttgc attttaccta aacctggaat atatggctct
     8101 tataaaatca atctcaggga taaggggaac catcggcgga aagcccggtg actctctcac
     8161 gcctgttgat atagtgaaat ttgcatctgc atacggtacc tggttaagga ggcggggcag
     8221 aggcccgctg cgcgttgtta ccggccgtga tgcccgtgtc tccggtccga tggttgccgc
     8281 gctggtcaac tcgtcactcc gaagcgtggg gatagacgtt attgataccg gaatggcatc
     8341 cacccctacc gttgaggtgg ctgtgacagg cctcggggca ggaggaggca tcatactgac
     8401 tgcaagccat aaccctgcac agtggaatgc actcaagctg ctcaatgaag agggagagtt
     8461 cctcagtgat gctgatggca gggaggtcct ggcactggcc gaagcagagg actttgatta
     8521 tgtgacctat gacaggctgg gtacggaaac cgttgatgac agctggactg aaaaacatat
     8581 caggatggta accgggatga cgctgactga ctgtgatgct atcaggaagg ccgggttcac
     8641 cgtggccgtt gatgctgtca actcggtggg aggcatagtc atccctgaac ttctgagagc
     8701 cctgggcgtc aaaaaggtga ttgagcttta ctgtgagcct gacggcctct ttccgcataa
     8761 ccctgaacca ttgcctgaga atctgtcaga aatctcccgc acagtgaagg agagcggcgc
     8821 ccatgtcggc ttcgtggttg accctgatgt tgacaggctg gccattgtat gcgaagacgg
     8881 gtccatgttc ggggaggagt atacactggt atcagtttca gactatgtcc tgggccatgt
     8941 caagggtccg acggtatcaa acatgtcatc aaccagggcg ctgagggacg tgacagagag
     9001 tcatggctgc agctatacct cagcacccgt gggtgaggtc aacgtggtag cggagatgaa
     9061 gcgcacagga gctgtcatag gcggtgaggg caacggagga gtcatatacc cggcattgca
     9121 ttacggacgt gatgccctgg tgggaatagc tctcttcctg acacacctgg caaaacaggg
     9181 tgtcagatgc agtgaattgc ggaagaggta tcccgattac catattgcaa agacaaagat
     9241 tgatctcccg ggcagcattg atatgaaaga gcttcgcgaa aagctggtca tgaaatatcc
     9301 ggaggctgac tgtgacaccc gcgacgggat aaaacttgac ctcccggcag ggtgggtgca
     9361 gataaggaaa tcaaacaccg aacctatcat gcgcatttat gccgaggaga gaacaatgga
     9421 acaggctgaa aaactggcag ctgatgttac tgccattgtt aaaaatctat aaatctggct
     9481 cgccagggca gatttctgac ttttttgaac ggtttttgtg ctcatccaac gggcagaagt
     9541 taatgaaagt taatcttttc ttaaaaagaa cagagtaagc ctaaatttgt aataatttca
     9601 cgcctttaat aaaataaaca gataattcag cagatgaaac ccacatgatt gaattcaaac
     9661 gcaaacacga aatgaatata attttcaatc atgtgcggtt ccatcatacc gctaagatca
     9721 aaattatatt atgatgaaaa agactattat cattacctca attattgtat tgctggctat
     9781 tatcgccatg ataatcatca gcaagctttc gggcaggaaa gacctgagca tattgtttac
     9841 cgaggttcaa tatggtgatt ttgaggtggt tgtcacaaca acgggggagt tgcaggcaga
     9901 ggtttcaact gatatcaagg gaccggaggg tttgagcagc cgcaacatga ggttcggagg
     9961 aatcaagata tctgatcttg taccagaagg taccgaggta aaggcaggtg attatgtcgc
    10021 cacgcttgac cgctcgagtg ttgacaacag tcttaaagat gagcttgaca ggctggagtc
    10081 tttagaaacc gacctgctga agaagcagct tgacaccaca atctctcttg gcaacctgag
    10141 agatgagctc ctgaatctca agttcaatat ggatgaagca gagattaccc tggagcagtc
    10201 acaatatgag cctcctacta ccatcaggca ggccaggatt tcacttgaca aggcccagcg
    10261 ttcctatgat caggcggtca gcaactacga cctgaaggtc aggcaggcca gggctgatat
    10321 cagggatatt gagcttcagg tttcaaaaca gcggagaaag gtggccgaga tgcaggatat
    10381 catccagaaa tttgtgattt cagctccggc tgatggtatg gttatctaca agagagagtg
    10441 gggtggcgct aaacgcaagg tgggttcgga aatcagtgcc tgggatccgg tagtggcaac
    10501 tcttccagac ctcacatcaa tgatatcaaa gacatatgtc aatgagattg acgtcagcaa
    10561 ggtaaaggcc ggtcagcgtg tcaggttaac cgttgatgcc ttccccgaga agtcatacac
    10621 cggtgtggtg ctcagtgttg caaacatagg cgagcagttg cccaacactg atgccaaggt
    10681 gtttgaggta atatcgaaaa ttgacggatc tgatccgata ttgaggccct ccatgactac
    10741 gggcaaccag ataattacca aaacattcag tgatgtttta tttattccgc ttgagacagt
    10801 gtttgccaca attgacagta tcccctttgt ctataagaag gatggaacac gccaggtggt
    10861 ggtgctcggt gagtcgaacg agaatgatgt gatagtggag cagggcctga aggagggtga
    10921 aaggttatac cttacaattc cggaagatgg cgaaaagttc aagcttcagg gtgatgatct
    10981 gattgccatt atcaaggaga ggaagaagca ggaggcagag gaggaagcca aacgtctgca
    11041 gaacggcaac cagcctcaga atatgatgcc cgaaaacatg accccggaga agatgcgtga
    11101 gttcatgcag aatctgactc ccgaacagcg tgaagccatg cgtcagatgc gcggttccgg
    11161 acgccccggc cagatgccgg gcagaccggc aggcggcagc gatacaacgg tcaggatgca
    11221 gagggaaaga acacccaacc agtaacagga ataaaagatt ctgaggcaaa taaacatgaa
    11281 ataacatgtt caaaagatac ggacacgaca tacgcatcgc ccttgagtca attctctcca
    11341 acaagctcaa atcagcactg accgcactcg ggatcatatt cggggtggcg gctgtgataa
    11401 gcatgctggc aataggcaag ggtgcacagc aggaaatcct tgaccagata aagatggtag
    11461 gggtgaacaa tatactcatc agccccatcg tgcaggagaa ggatgccgca gaggaggatg
    11521 gagagaaaca gctgaacaaa ttctcgagag gccttaccct gcttgatgtt gaggcaatac
    11581 gcagcaccat ccccgcggtg aagagaatca gccccgagat atcattcaac tccaccatcg
    11641 ttcttagcgg tatgcgccat acggccaagc tggtaggggt atcaaatgat tattttgagc
    11701 tttataatct tcccctttcg cagggcgcct tctttaatga ctaccaggaa gaatacggaa
    11761 tccaggtttg tatcataggt gccaacatca gggccaagtt cttcagccag gttgacccta
    11821 tcggccagta tctcaagttt gacaacgtct ggctgaaggt gatgggtgtg cttgaaaaga
    11881 ccaatgtttc cctgaccgga tttgaggaac agggagtaaa cgtttacaat gacaacgttt
    11941 atattcctat caagacaatg ctcatgcgtt accagaaccg ggcgcttgtc aacaccaagc
    12001 tggcaagcga ggcatcggtt catgtctttg gtggcggaag aggagggcgc ggacgggtgg
    12061 ttgtcagcag taccaatgcc tctactgcca acgccgagac caactataac cagcttgaca
    12121 agatagtggt tcaggttgct gagaccgaac agcttacccc tacaactgaa gtcctcagca
    12181 gaatgatgct caggcggcac tcaggcgtga aggactttga gatcacggtt ccggagttgt
    12241 tgctgaagca gcagcagagg acaaaggaca ttttcaacat cgtgctgggt gccattgcca
    12301 gcatctcact tatcgtcggc ggaataggca tcatgaacat tatgtttgct tcagtgatgg
    12361 aaaggatcaa ggagatagga acaaggatgg ccattggcgc caaaaagatg gatattgtgg
    12421 tgcaattcct gtcagaggct gtactgatca gtgtctccgg aggtttcatc ggcatattcc
    12481 tcggaataat actggcagtt ctgatacaga agatagcggg tataaccacc attgtttcgt
    12541 tcttttccgt tgtggtggct ttcggcgttt ctgccgccgt gggggtcatt ttcggctatt
    12601 cgccggcaaa gagagcctcc gaaagagacc cgattgaatc acttcgttac gaataataat
    12661 atcattattg atatgaaaag cataaaagca ctttcattga ccgttttgtt ttcagcgttg
    12721 gttgccctcc cggcaatgcc gcaggaagtc aggctcctta cgctggatga ggttattaag
    12781 cttgctgcag atcagtcgcc caatgcatac atagcaaagc accgttttcg tgccagctac
    12841 tggcaataca ggacttttgt tgcggaatac cgccctacgt tgtctctttc cggcaatatg
    12901 ccggactaca gtactgccta cagcagggta tggaactccg tagcgcagca gtgggagtat
    12961 gcttctacga acgttctgca gacctcaggc aacctgtcgc ttgcgcagaa catcgggctg
    13021 acgggcggtt ctgtctcgct cttctctgac ctgaattacg aaaagaattt cgagaccggt
    13081 ggagaaaggt acattacatc accgcttaat gtcaggctga cccagcctct cttcaggtat
    13141 aacgagctga gatggcagaa gaagatagag cctctcaggt acgaggaggc acgtaagacc
    13201 tatctgaggg atgtggagaa cgttcatatg gtagcggtgc agtacttctt caacctggca
    13261 ctggcccaga taaacaagga gattgccgag acaaacatgc agaatgcaga taccctgtac
    13321 cagattgcca aaggccggta taacctgggt acgattgctg aagatgaact gttgcagatg
    13381 gagctttcat atcttaatgc cggaacagcc atcaatgaat cagaaatgaa cctccgtgac
    13441 cgtgagctta agttgaggtc attccttggt tttaaccagt cggttcgtct ggagctgata
    13501 atccccagtg agataccgga tctggaagtt gacgtcagcg aggttctgag actggctgaa
    13561 gagaacaacc cggaactgat tgcccttgag cgtcagcttg ttgaggctca gagctcggtt
    13621 gcacaggcaa gggcagagaa aggtttgaat gcaaacctga cggcatcata tggtctgcgt
    13681 gatcaggatc cgttgcttca acaggcctat acgcatccta accagcagca gacaataagg
    13741 gtctcattct cccttcctat tcttgactgg ggtcagggaa ggggccgtta caagatggcc
    13801 cagtcaagtg aggagctgac ccgcgtgcag gtggagcagt cacgcattga ctttgaacag
    13861 aacctgcttc ttgacgtgca gcagttcaac atgcaggatg accaggtgat cattgccgcc
    13921 aagtctgaca cagtggccgc caaaatgttc gaggtgacca agca
//
LOCUS       DAOPBK010000060        13314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4646_length_13314_cov_5.167735, whole genome shotgun sequence.
ACCESSION   DAOPBK010000060 DAOPBK010000000
VERSION     DAOPBK010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4646_length_13314_cov_5.167735"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /gene="thyA"
                     /locus_tag="PLA17_04980"
     CDS             complement(<1..432)
                     /gene="thyA"
                     /locus_tag="PLA17_04980"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPK84801.1"
                     /translation="MKQYLDLLSHVMEHGIEKRDRTGTGTVSTFGYQMRFDLSGRFPL
                     MTTKKLHLKSIIHELLWFLSGSTNVRYLQENGVRIWNEWADPDGNLGPIYGYQWRSWP
                     ASDGRSIDQVKNVVTSLKNNPDSRRHIISAWNVGEIEKMALP"
     gene            complement(448..2403)
                     /locus_tag="PLA17_04985"
     CDS             complement(448..2403)
                     /locus_tag="PLA17_04985"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPK84802.1"
                     /translation="MKVLRILIAAGLLIPVPLLSKEYKVKSPDGKITLLAETTAGINL
                     TVSYDNKEIITGLSPELQLEGVTIPGQRPAITKAVTSSANNMLVPVVPRKNSRVPDIF
                     NALELRFRGGSSLEFRVYDDGVAYRFRTAMKGDIKVMDEQFAFSLPGGAGVYWPLEEG
                     FMSHNECTFHPASMDTIGPEHLASLPALFTSGSLNILLSEADIRDYPGLWINGDGRGG
                     VKGVLPAYPAEVKAYNDRNVYVTGREEYLAVTPGTRTFPWRLFIITPDDAGLVESEMV
                     FRLGAPPAPGTDFSWVRPGKVAWDWYNANNIYGVDFRAGLNNDTYRYYIDFASENGIE
                     YVILDEGWYKLGDVMEQSPGFDVGELCEYAASKNVGIILWVVWKTFHDKIDEALIQFE
                     KWGVKGIKVDFMQRDDQWMVNYYHEVAEKAARHKMLVDFHGSYKPDGMERAWPNVITR
                     EGVKGMENNKWSTDCDPEHDLILPFTRMVAGAMDYTPGAMVNMQQRDFRPIFYRPASQ
                     GTRVHQMAMYVVFESPLQMLADSPSNYKRNQECTSFIAAVPVTWDETRVIEARKGDNI
                     VMARRHGTTWYLAAMNDWKPFVTEVDLSFLPAGEYSMEIFSDGINADRHGEDYKHITR
                     NAAAGEKITLSLAPGGGWVAKITPKAR"
     gene            complement(2418..3023)
                     /locus_tag="PLA17_04990"
     CDS             complement(2418..3023)
                     /locus_tag="PLA17_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HPK84803.1"
                     /translation="MQNKLIRLKVIGISYSQTQSGAYALILAEENGERRIPIIIGGFE
                     AQAIVIKLENLEPPRPLTHDLFRSFAAACGVTVEQVLINRLREGIFYSEIVCSDGERR
                     FNIDSRTSDAVALALRFECPIFIEPQILNEAGIVMSATTGDEEEKQPKLQKEKRERVS
                     SEYSVFSVDELNDMLSDAISREDYEQAAAIRDEMARRKGEG"
     gene            3267..3575
                     /gene="rplU"
                     /locus_tag="PLA17_04995"
                     /pseudo
     CDS             3267..3575
                     /gene="rplU"
                     /locus_tag="PLA17_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            3932..4192
                     /gene="rpmA"
                     /locus_tag="PLA17_05000"
     CDS             3932..4192
                     /gene="rpmA"
                     /locus_tag="PLA17_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPK84804.1"
                     /translation="MAHKKGVGSSRNGRESESKRLGVKLFGGQAVKAGNIIVRQRGTV
                     HNPGENVGLGKDHTLFALVDGTVEFKKKRDNKSYVSVKPAAE"
     gene            4298..5572
                     /gene="serS"
                     /locus_tag="PLA17_05005"
     CDS             4298..5572
                     /gene="serS"
                     /locus_tag="PLA17_05005"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPK84805.1"
                     /translation="MLTINLIREQSSFVIERLKIKNFDAAEIVQNVLDLDRRRRELQA
                     ESDSIQAELNTLSREIGAMMKAGRRDEADAARQGVVAMKEKQKQLSETMEVVSDEMQT
                     ELVRLPNLPHALVVPGKSADDNVVVRSGGTIPHLYEGALPHWDLIRKYDIIDFDLGVK
                     ITGAGFPVYKGKGARLQRALINFFLDEGEKAGYREVEPPIVVNEESGFGTGNLPDKEG
                     QMYHIGIDNLYLIPTAEVPVTNIYRDVILDAAVLPVKNMAYTPCFRREAGSWGAHVRG
                     LNRLHQFDKVEIVQIAHPEKSYESLDEMVSHVESLLVKLELPYRIVRLCGGDLSFTSA
                     MTYDFEVWSVGQQRWLEVSSTSNFETFQANRLRCRFREKGARQTTLCHTLNGSALALP
                     RIVAALLENNQTPEGIKIPSVLVPYTGFGMIS"
     gene            5578..6462
                     /locus_tag="PLA17_05010"
     CDS             5578..6462
                     /locus_tag="PLA17_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPK84806.1"
                     /translation="MKKSQQAYLYASLSVVLWGTIPTAFKIALSELNIVTMLGITTLI
                     STAVLFAIMILTGKLRLLRQTTGRELLWSALLGLVNPVAYYLILLTAYSRLPGQVAQP
                     INMIWPIILVFFSIPLLGQRIPARSFLALAISLAGVYIISSQGTPLSPGRSDTLGVIL
                     ALISSVLWALYFVLNVRDRRDESVKLFTGFLFGSAYMIILMASVGSFNQPLSFRGVAA
                     SVYCGFFEMGITFWLWLKALQLSETTDKVSNLVYFAPFISLILLHFIIDEPVYYTTPA
                     GLLLIIGSVIFQNRTKKT"
     gene            6509..7843
                     /locus_tag="PLA17_05015"
     CDS             6509..7843
                     /locus_tag="PLA17_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPK84807.1"
                     /translation="MKRVFIVSLLIIPLLFGCKKDPEPSKTPTLEERARDGLYDLMKY
                     VYLWYSNMPTVKTSDYTGPEQLLEAVRYRPRDKWSFIADYESFMASMQGSFVGHGIRM
                     GLDNANNVRVVSLYEGSDLWPKGVRRGWIVKEINGTAIAPIFISGNNTAYNNLMGPPT
                     AGTSNTFTFIDPDNQEVTYTSVKSSFTINSVTASKVLDLTGGKTGYLCFETFIEPSEQ
                     ELNEAFADFKASNVTDLIIDLRYNTGGYMSIAQQLASLVLTWDDTTKVCYKLTYNNLV
                     GPEWNESFNFVRTESPLGLDRVVFITTRSSASASEVVINSLMPWVDVKLVGDTTHGKP
                     AGMNIWGYPFPSDAVPKPDYKYVFAPITFEYLNSDDQGRFYDGIKPDVRAADDITHDF
                     GDPEEASLQAAINLLEGKKSVTAEPVRRPTIWSEGNQLPTDLILGPSKEIGK"
     gene            7840..8403
                     /gene="ruvC"
                     /locus_tag="PLA17_05020"
     CDS             7840..8403
                     /gene="ruvC"
                     /locus_tag="PLA17_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPK84808.1"
                     /translation="MNDQQSGRLILGIDPGTTVTGYGLISVSGKSPSLITLGIIDLRK
                     KEDHFRKIRTIFEETLGLIDRYHPDEFAIEAPFFGKNVQSMLKLGRAQGAAISAALYR
                     DLPVFEYAPRMIKMSITGRGQASKEQVASMLQTLLAFRGESMVLDATDALGAAMCHFY
                     QSSKPVADRKYRNWGDYIKKNSDRIKQ"
     gene            complement(8393..9103)
                     /locus_tag="PLA17_05025"
     CDS             complement(8393..9103)
                     /locus_tag="PLA17_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPK84809.1"
                     /translation="MFHIKDIADDFLSLFLPRPCLSCGVPLVRGEEVLCTGCMLDMAR
                     TDFHLRRGNMLEQAFWGRCIVERAAAYSVYNRGSRIRKLIHLLKYSGRKDIGIMLGRL
                     YGSVLAESGFTDGIDMIIPVPLDPVRERRRGFNQSKCIAEGIASHCCLPVRSDILRRT
                     GKSGSQTKRGRYERWENVEGLFTVTRPREIAGRHLLLVDDVITTGSTVEACVTALQSA
                     GGVRVSVAALAAAQKLTV"
     gene            9214..9522
                     /locus_tag="PLA17_05030"
     CDS             9214..9522
                     /locus_tag="PLA17_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84810.1"
                     /translation="MKKWMIGSLFLIVMMFVAAVVYAQTTVKEKKHTKSECVFVDKNK
                     DGKCDICGSTADACKKEAVKGKECSGCPSAAACGENKGEVVPAKEGTKATTPCCAGKK
                     "
     gene            complement(9671..10069)
                     /locus_tag="PLA17_05035"
     CDS             complement(9671..10069)
                     /locus_tag="PLA17_05035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPK84811.1"
                     /translation="MASIFTRIINGEIPCYRVAENDRYIAFLDVRPLKAGHTLVVPKK
                     EVDYIFDMDDEALSGMIIFAGKVARAMKEVIECNRIGVAVIGLEVPHAHIHLIPISRE
                     GDMLFTNPRVSLSPEENERLAKEIGAHVKL"
     gene            complement(10082..10555)
                     /gene="greA"
                     /locus_tag="PLA17_05040"
     CDS             complement(10082..10555)
                     /gene="greA"
                     /locus_tag="PLA17_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPK84812.1"
                     /translation="MMSEFVYVTEEGLQKMKEELDQLRNERPAVARLIADARDKGDLT
                     ENAEYSAAKEVQGMLEMKIAKLEDTVARSRIIDSSRITTKTVQILNRVKILNKANGSL
                     MEYQLVPEGEANLKEGKIAATSPIAQGLMGRKVGEVVEIRVPAGTIPFEIIEISI"
     gene            complement(10676..11317)
                     /locus_tag="PLA17_05045"
     CDS             complement(10676..11317)
                     /locus_tag="PLA17_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84813.1"
                     /translation="MEDVGNLIFYILLGIIALVGSFQGKGKKKSGKPGPVQRQPGTLT
                     TRPPVKTTAQTARPAPSTIRQERQTPFTVREERPVPGPSPWTMPAGQIIEGRYEEPMA
                     RDFSREGNIRNDMAGAFSSEGSVASGMGEAFANEGSIEDSMAAAFASEGVSALGELAT
                     GDFFQADITDGEISDAPGYDYEALPGTDIHKEGFDLKRAVIYSAILDRKEYSR"
     gene            complement(11323..11862)
                     /locus_tag="PLA17_05050"
     CDS             complement(11323..11862)
                     /locus_tag="PLA17_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84814.1"
                     /translation="MKRGLVLLRFVAAVIVLLFVVEATECNAQKNATKRFERETFGKS
                     RRSKPVKESGESRAAAKAMKEQARKEARREKEDEKALKEKRERHYQIQSEETKMRMEN
                     NSRNTADRYKVKRQKERREQTKPELKKPQQPVPDRAGPKPKTKDPSKQPVLKQQKKKA
                     KTINPKKQPKIKQHKVKKY"
     gene            11927..12412
                     /locus_tag="PLA17_05055"
     CDS             11927..12412
                     /locus_tag="PLA17_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84815.1"
                     /translation="MSVSNLRIFLLFSLFTLLIISCDRDKNDVIPDITVDFTITSSDP
                     EFFDLFNAPGTSALISSAHRHLGLGTAGFDGNGIIVYSTGLHGFEFPAFDRTCPHCYA
                     NNEVSVAVTVDGVYAICPECSTNYALGGSGLPQSGPGHYYLKNYRTSFSGYSIRVWNR
                     R"
     gene            complement(12419..>13314)
                     /locus_tag="PLA17_05060"
     CDS             complement(12419..>13314)
                     /locus_tag="PLA17_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPK84816.1"
                     /translation="ILEEDPQKWHRTVSEMKGISEETTTGVHRLYQMLEAGELLVPAI
                     NVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVCGYGDVGKGSARSMRSYG
                     ARVIVTEIDPICALQAAMEGFEVRTVEESLAEGNIFVTATGNRDVITIEHMEKMKDQA
                     IVCNIGHFDNEIQVDLLNAYKGIRKINIKPQVDKYLFPDGHAIFLLAEGRLVNLGCAT
                     GHPSFVMSNSFSNQTLAQIELWTKEHAIGVYRLPKQLDEEVARLHLAQLGVSLTKMTR
                     AQSDYIGVPEGGPYKPDHYRY"
BASE COUNT         3203 a         3603 c         3224 g         3284 t
ORIGIN      
        1 cggaagggcc atcttttcta tctcccctac attccaggct gagatgatat gtcttcgcga
       61 gtcgggatta ttcttcagtg atgtgaccac gttctttacc tggtcaatcg atctgccgtc
      121 acttgccggc catgatcgcc actgataacc gtagatgggg ccaagattac cgtcagggtc
      181 agcccactca ttccatatcc tgacaccgtt ttcctgcagg tatcgcacgt tggtgcttcc
      241 gctgaggaac cataacagct catgaatgat tgatttcagg tgcagctttt tggtggtcat
      301 cagcggaaac cgcccggaca gatcaaagcg catctggtag ccgaaggtgc tcaccgtacc
      361 ggtacctgtc cggtctctct tctcaatgcc atgttccatg acatggctca gcagatcaag
      421 atattgtttc atagaattcc ggactatcta tctggctttg ggagtgatct ttgccaccca
      481 tcctcctccc ggagccaggc tgagagtgat cttttctccg gcagcagcat tgcgggtaat
      541 atgtttgtaa tcttctccgt gcctgtcagc gttaattccg tcggagaata tctccatgct
      601 gtattcaccg gccggcagga acgaaaggtc aacctctgtc acaaacggtt tccagtcatt
      661 cattgcggcc aggtaccatg tagtaccgtg cctgcgcgcc atgacaatat tatcaccctt
      721 cctggcttca atcaccctgg tttcatccca ggtgaccggc acagcggcaa tgaaggaggt
      781 gcactcctgg ttgcgtttat agttggaggg actgtcagcg agcatctgca gggggctctc
      841 gaaaaccaca tacatagcca tctgatgtac cctggtgccc tggctggccg gtctgtagaa
      901 tatcggcctg aagtcgcgct gctgcatgtt taccatggca cccggggtat agtccatcgc
      961 gccggctacc atgcgggtaa agggcagtat caggtcatgt tccggatcgc aatccgtact
     1021 ccacttgttg ttctccattc ccttgacccc ttcgcgggtg attacattgg gccaggctcg
     1081 ctccatcccg tcaggcttgt aggagccgtg gaaatcaacc agcattttat gtctggcagc
     1141 cttttcggca acctcgtgat aatagttgac catccactgg tcgtcacgct gcatgaaatc
     1201 aaccttgatc cccttcactc cccacttctc aaactgtatt agggcttcat caatcttgtc
     1261 atgaaaggtt ttccagacaa cccacagaat gattcccacg tttttcgatg cggcatattc
     1321 gcacagctca ccgacgtcaa atccgggcga ctgctccata acatcaccca gtttgtacca
     1381 gccttcgtcg aggatgacgt attcaatacc gttctccgat gcaaaatcta tatagtacct
     1441 gtatgtgtca ttgttgagtc cggccctgaa atcaaccccg tagatattgt ttgcgttgta
     1501 ccagtcccat gccacctttc cgggtctgac ccagctgaag tctgttcccg gtgccggtgg
     1561 ggctcccagc ctgaagacca tctcactctc aaccaggccg gcatcatcag gggtgatgat
     1621 gaagaggcgc cacgggaagg tgcgtgtgcc cggcgtcact gcaaggtact cttcgcgccc
     1681 ggtcacatag acgttgcggt cattgtacgc tttcacttca gcagggtatg caggaagcac
     1741 acccttcact cccccgcggc cgtctccgtt tatccacaag cccgggtagt cacgtatgtc
     1801 agcctcactg aggaggatgt tcaacgagcc cgaggtaaaa agcgccggca ggctggccag
     1861 gtgttccggg ccgattgtat ccattgatgc ggggtggaag gtgcactcat tatgtgacat
     1921 gaagccctct tccagtggcc agtatactcc ggctccgccg ggaagcgaaa aggcaaactg
     1981 ttcatccatt accttgatgt ctcccttcat tgccgtcctg aacctgtatg ccacaccgtc
     2041 atcataaacg cggaactcaa gtgacgagcc gcccctgaag cgaagttcaa gcgcgttgaa
     2101 aatgtcaggc acccggctgt tcttccgtgg caccacggga accagcatat tatttgctga
     2161 tgaggtgaca gccttcgtta tggccggacg ctgcccggga atggtcaccc cttccagttg
     2221 cagttcgggc gacagcccgg tgattatctc cttgttatca taactgacgg tgagattaat
     2281 ccccgccgtg gtctcggcaa ggagtgtgat ctttccgtcc ggtgatttca ccttgtactc
     2341 ctttgacagc aggggcacag gtatcagcag ccctgctgca atcaaaatcc ttaaaacctt
     2401 catctctccc tgattaatta tccttctcct ttccttctcg ccatctcgtc ccggatagct
     2461 gccgcctgct cataatcctc acggctgatt gcatcggaaa gcatatcatt aagctcatcc
     2521 acggaaaata cgctgtattc gctgctcacg cgctctctct tctccttttg cagtttcggc
     2581 tgcttctcct cctcatcccc ggttgtggcg ctcatgacaa tgccggcctc attcagaatc
     2641 tgaggttcaa tgaatatcgg acattcaaac ctcagtgcca gtgccacggc atcagaggta
     2701 cgcgagtcta tgttaaaacg cctttcgccg tcactgcaca cgatctccga atagaatatc
     2761 ccttcccgga gccggttgat gagtacctgt tccaccgtta caccacaggc tgcggcaaag
     2821 ctgcggaaga gatcatgtgt gagcggcctt ggcggttcca ggttttcaag cttgatgact
     2881 atggcctgag cctcaaaccc cccgataatt ataggtatac gccgttcccc gttttcctcc
     2941 gccagtataa gggcataggc gcctgactgt gtctggctgt atgaaatgcc tataaccttc
     3001 agacgaatga gtttattctg catagtttac cagattttct gcctgacagg ccaataagac
     3061 aaatataaat aatatggctc tttatctcct ccccttcccg attttttatc cacattgtat
     3121 tggtgataaa ttattgtcac cgatttgtac ataagttaat ttttatatct ttgcagtccg
     3181 aaaaaccaag atccggcggg gcctgcagaa gtgtcatgca cagaggagca tgaccgcctc
     3241 acggagtaac ataagagaga ttgaaaatgt acgcaatcgt tgaaattgcc ggtcagcagt
     3301 tcaaggtaga agcaggacag aaaatatatg ttcaccatct tgatgcagct gaaggcgaca
     3361 gtgtagagtt tgaaaaagta cttcttacag acaatgatgg tgtggtcacc attggtgaac
     3421 cccttgttcc caacactgtt gttgagggca aggtcctcgg tcatgtaaga ggagacaaaa
     3481 tcatcatctt caagaagaag agaaggaagg gttatcagaa gaggaccggg caccgccagg
     3541 aattcacaca ggtggagatt ctgagaatag gcgccgccat tgagcctgag aagaaaaccg
     3601 caccaaaggc agagaaaaaa gctgcagaag tacccattgc agaagaggtg aaggtagaaa
     3661 agaaaactga aaaagcacca gcagctgaaa aggtgaagac aaaaaagaaa gttgccgaga
     3721 caccggctgc agaagaggtg aagacgaaaa agaaagctgc tgaggaaccg gctgcagaag
     3781 aggtgaaggc agagaagaaa aaagctcctg caaaaaaagc cgccgccagc aaggaaacgg
     3841 ctgagaagaa ggaaaccgta aagaaggctc cggcaaaaag cacaaagaaa ccggcaaagg
     3901 aataagcgat aaacagtaaa aagaaaacga tatggcacac aagaaaggtg taggcagttc
     3961 acgtaacggc cgcgaatcag aaagcaaaag gcttggtgtc aagctcttcg gagggcaggc
     4021 agtaaaggca ggcaacataa tcgttcgcca gcgtggaacc gtccacaatc ccggtgaaaa
     4081 cgtcgggctc ggaaaggatc ataccctctt tgccctcgtt gacggaaccg tggagttcaa
     4141 aaagaagcgt gacaacaaat cttacgtctc ggttaaaccc gcggcagaat aggatactga
     4201 caacttacag caactcctga aaaatacgct gaggcgcata ttttcaggag tttttgtttt
     4261 aattttacca cgcttatcac caaagattcc ttccggtatg ttaaccatca acctgattcg
     4321 tgagcaatcg tcgttcgtca ttgaacggct gaaaatcaag aacttcgatg ctgcagagat
     4381 agtacagaat gtgcttgatc ttgaccgccg ccgccgcgag ctgcaggctg agagtgactc
     4441 catccaggct gagctcaaca ccctgtcacg tgagattggt gccatgatga aggcagggcg
     4501 cagggatgag gctgatgctg cccgtcaggg cgtagtggct atgaaggaga agcagaagca
     4561 gttgtcagag accatggaag tggtttcaga cgagatgcag acagaactgg tcaggctgcc
     4621 caacctgccg catgcgctgg tagtcccggg gaaaagcgcc gatgacaacg tggtggtgcg
     4681 cagcggcggc accatacctc atctttatga aggagccctc ccccactggg acctgataag
     4741 gaagtatgac ataattgatt tcgaccttgg ggtcaaaatt accggcgcgg gttttcccgt
     4801 ctacaagggc aagggtgcca ggctccagag agcactcata aacttcttcc tcgacgaggg
     4861 tgagaaggcc ggctaccgtg aagttgaacc gcccatagtg gtcaatgagg agtcgggctt
     4921 cggcacaggc aatctgcccg acaaggaggg gcagatgtat cacattggca ttgataacct
     4981 ttatctgata cccactgctg aggtgcccgt aacaaatatc taccgcgatg tgatccttga
     5041 tgctgccgta ctgccggtga agaacatggc atacaccccc tgcttccgcc gtgaggcggg
     5101 ctcgtggggc gcacatgtga gaggcctcaa cagactgcac cagtttgaca aggtcgagat
     5161 agtacagata gcacatcctg aaaaatctta tgagtcactt gacgagatgg taagccacgt
     5221 ggaaagcctc ctggtgaagc tggagctgcc ataccgtatt gtgaggctct gcggcggcga
     5281 cctctcattc acctctgcaa tgacatatga ctttgaggta tggtctgtag ggcagcagcg
     5341 ctggctggag gtgagctcaa cctctaactt cgagactttc caggccaaca ggctcaggtg
     5401 ccgcttccgt gaaaaaggag ccagacagac caccctctgc cataccctta acggaagcgc
     5461 attggcattg ccgcgtatcg tggccgcact tctcgagaac aaccagacac ctgagggaat
     5521 aaaaataccg tctgtgctgg taccctacac agggttcgga atgatcagct gagagagatg
     5581 aaaaagagtc agcaggcata tctttatgcc tccctctcag tggtgctatg gggtaccatt
     5641 cctaccgcct tcaagatagc cctttccgaa ctgaacatcg tgaccatgct ggggatcacc
     5701 accctgatat ctacagcagt tctgtttgca ataatgattc ttacaggcaa gctccgcctc
     5761 ctccggcaga cgacaggccg tgagctgctc tggtcagccc tgctggggct ggtcaatccc
     5821 gtcgcatact atcttatcct gctgaccgca tacagccgcc tgccgggcca ggtggcacag
     5881 cctatcaaca tgatatggcc catcattctt gtcttctttt ccataccgct gctggggcaa
     5941 cgcatacctg cccgcagttt cctcgcactc gcaatcagcc tcgccggggt atatataata
     6001 tcatcacagg gcaccccgct cagcccgggc aggtcagaca ccctgggcgt catcctggca
     6061 ctcatcagca gcgtcctctg ggctctttat ttcgtcctta acgtgcgcga caggcgcgat
     6121 gagtcggtaa aactgtttac cggtttcctg ttcggttcag cctatatgat catcctgatg
     6181 gcatccgtgg gctccttcaa ccagcctctg agcttcaggg gggtggcggc atcggtctac
     6241 tgtggcttct tcgagatggg aattaccttc tggctctggc tgaaagcact tcaactctcc
     6301 gagacaaccg acaaggtgag caacctggtt tatttcgctc cgttcatctc cctgatactg
     6361 ctgcacttta tcatagacga acccgtttat tatacaaccc cggcaggact gcttctcatc
     6421 ataggaagcg tcattttcca gaacaggaca aagaaaacct gaatcaaccg gcacattttt
     6481 tgttactttt acatcaaatt tactcattat gaaaagagta tttatagtat cacttctgat
     6541 catacccctg cttttcggat gcaaaaaaga cccggagccg tctaaaactc ccaccctgga
     6601 agagagggcc cgcgacggtc tgtatgatct gatgaaatat gtctatctct ggtacagcaa
     6661 catgccaact gtcaagacat ctgactacac cggcccggag caactcctgg aagccgtgag
     6721 atacaggccg agagacaagt ggagcttcat tgcagactat gagagtttca tggcttcaat
     6781 gcagggcagc ttcgtgggtc acggaatcag aatgggcctt gataatgcca acaatgtcag
     6841 ggtggtaagc ctttacgaag gctcagacct ctggccaaaa ggagtaagaa ggggatggat
     6901 agtaaaggag attaacggca cagccattgc ccccatcttc atctcgggca acaacacagc
     6961 ctacaacaac cttatgggtc cccccacggc aggaacgtcc aacacgttta cattcatcga
     7021 tcctgataac caggaggtga cctatacctc cgtcaagtcg agcttcacca tcaactccgt
     7081 gacggcatca aaggtcctcg acctcaccgg aggcaagacg ggttacctct gctttgagac
     7141 attcattgaa ccgtctgaac aggagcttaa cgaagccttt gccgatttca aggccagtaa
     7201 cgtcaccgac ctcataattg acctccggta caataccgga ggatacatga gtatagccca
     7261 gcagcttgcc agccttgtgc tgacatggga tgacaccaca aaggtgtgct acaagctgac
     7321 atacaataat ctggtgggtc ccgagtggaa tgagagcttc aatttcgtca ggacggagag
     7381 ccccctgggc cttgaccgtg ttgtctttat caccacccgc agttcggctt ctgccagcga
     7441 ggtggttatc aacagcctga tgccctgggt tgatgtgaag cttgtgggtg acacaacaca
     7501 tggcaagcct gccggcatga acatctgggg atatccgttc ccctctgacg ctgtgccgaa
     7561 accggattac aagtatgtct ttgcaccaat aacctttgag tatctgaact cagatgacca
     7621 gggccgtttt tatgacggga taaagcctga tgtgagggct gctgatgaca taacacatga
     7681 ttttggcgat cccgaagaag cctccctgca ggcagcaata aacctgcttg aaggaaagaa
     7741 gtcagtgacg gcagagcccg tcaggcgacc caccatatgg tctgaaggaa accagttgcc
     7801 caccgacctc atcctgggtc catcgaaaga gatcgggaag tgaatgatca gcagagcggc
     7861 aggttaattc tcggcattga ccccggtaca accgtcacgg gttacggcct tataagcgtg
     7921 tcgggaaaaa gcccgtcact catcaccctt ggcatcattg acctgcgcaa gaaggaggat
     7981 catttccgaa agatacggac catcttcgag gagacgctcg ggcttattga caggtatcat
     8041 cctgatgagt ttgctatcga ggctcccttt ttcggcaaga acgtacagag catgctcaag
     8101 ctgggcaggg cacagggtgc ggcaatatca gctgcgctct acagggacct gccggtattc
     8161 gagtatgcac cgagaatgat caaaatgtct atcaccggca gaggccaggc cagcaaggag
     8221 caggtggctt ccatgctgca gaccctgctt gccttccgcg gcgagtctat ggttcttgac
     8281 gctaccgatg ccctgggagc agccatgtgt cacttctacc agagcagcaa accggttgca
     8341 gacaggaaat acaggaactg gggcgactac ataaaaaaga acagcgacag aatcaaacag
     8401 taagcttctg tgcggcagcg agggctgcca cgctgaccct gacaccaccg gcagactgga
     8461 gcgcggttac acaggcttcc acagttgaac cggtggtgat tacatcatca accagcaaca
     8521 gatgccggcc ggcaatctcc cggggtctgg tcaccgtgaa gagtccttcg acattctccc
     8581 acctctcata ccttcccctt tttgtctgtg agccagactt gcccgtccgg cgaagaatat
     8641 cgctccttac cggcaggcag caatgtgaag caattccctc ggcaatgcac ttactctggt
     8701 tgaatccgcg gcgcctttcc cttaccggat cgagcggtac ggggatgatc atatcaatcc
     8761 cgtcagtgaa tccgctttcc gccagaacag agccgtacag cctgccaagc atgatgccga
     8821 tatccttcct gcctgagtac ttcagcaggt gaataagttt tcttatgcgg ctaccgcggt
     8881 tatacaccga ataagccgct gccctttcca caatgcacct gccccagaaa gcctgctcga
     8941 gcatgttgcc gcggcggagg tgaaagtcag tacgtgccat gtcaagcatg caaccggtac
     9001 agagcacctc ttctcccctg accagcggaa caccacacga gaggcatggc cgcgggagga
     9061 acagactcag aaagtcgtca gcaatatctt taatatgaaa cacttcacag cgagaagcag
     9121 aatcaaagat atgatattta taacacctcc gtaacggtaa ttaaaatttt tctatattgc
     9181 ggccgtttaa taaacaaacc aattggcaca agtatgaaga aatggatgat tggcagcctg
     9241 tttctcatcg tgatgatgtt tgtggctgca gtggtctatg ctcagactac cgttaaggaa
     9301 aagaagcaca ccaaaagtga gtgtgtcttt gtcgataaga acaaggacgg taagtgtgat
     9361 atatgcggtt caaccgcaga tgcatgcaag aaagaagctg tgaaaggcaa agagtgctcc
     9421 gggtgcccgt ccgctgcagc atgcggcgag aataagggtg aagtggttcc ggcaaaggaa
     9481 ggcactaagg caaccactcc atgctgcgca ggtaagaaat aaccagacag cagataataa
     9541 aaagagggtg tctcaaaggg gcaccctttt tcttttgtgc ttactgttca tttccagccc
     9601 tggtcagcga ccggggcttt tttttgaccg gacatggcag gtgccggttt tattgttgtc
     9661 agtaccgcga tcagagcttt acatgtgctc caatctcttt tgccagacgc tcattttctt
     9721 ccggtgacaa tgaaaccctg gggttggtaa agagcatatc cccctcacgg gagatgggaa
     9781 taagatggat atgggcatga ggcacttcaa gcccgatcac tgccacacca atcctgttgc
     9841 attctatcac ctccttcatt gccctggcca ctttcccggc aaagattatc attccggaga
     9901 gtgcctcatc atccatatca aagatataat ccacttcctt cttggggact accagggtgt
     9961 gaccggcctt aaggggcctg acgtcaagaa atgcaatata gcggtcattc tctgccaccc
    10021 tgtaacaggg tatctcaccg ttgatgatgc gtgtaaagat tgatgccatg atatcttgtt
    10081 ttcagattga tatttcaata atctcaaacg ggatggtgcc ggcaggtacc ctgatctcca
    10141 ccacctcacc cacttttctg cccatcaatc cctgagcaat tggtgatgtg gctgctattt
    10201 ttccctcctt gaggttggct tcaccctccg gcacaagctg gtattccatc agtgagccgt
    10261 ttgccttatt gagaatcttg accctgttca gaatctgtac cgttttggta gttatccttg
    10321 agctgtcaat aatgcgtgac ctggctaccg tgtcttcgag tttggctatc ttcatctcga
    10381 gcataccctg tacctccttg gcagcagaat actccgcgtt ttctgtaagg tcacccttat
    10441 cacgcgcatc ggcaatcaat cttgcaacag ccggcctttc attacggagc tggtcaagct
    10501 cttccttcat tttctggagc ccttcttcag ttacataaac aaattccgac atgatttccc
    10561 gtaaatttgt taaaaaataa gaagaatccc ggcattaccg gaactcttca gagaacaaat
    10621 ataaaaaatt tctaaataaa aaacaaattc cctccgcctg gtaattatca gataatcaac
    10681 gtgagtactc ttttctgtca agaattgctg aataaatcac agcccttttc aggtcaaatc
    10741 cttccttatg aatatcagtg ccgggcagag cttcataatc atagcccgga gcgtcactta
    10801 tctcaccatc tgttatatca gcctgaaaga aatcacccgt tgccagctca cccagggctg
    10861 agacaccttc tgaagcaaaa gcggcagcca tggaatcctc aatggagccc tcattggcga
    10921 aggcttcgcc catcccggat gcgactgacc cttcgctgct gaaggcaccc gccatgtcat
    10981 tcctgatgtt tccctcccgg ctgaaatcac gggccatcgg ctcttcatat ctcccctcaa
    11041 ttatttgtcc ggcgggcata gtccatggtg aaggtccggg aacgggtctc tcctctctga
    11101 cggtgaaagg cgtctgtctc tcctgcctta tagttgaagg cgccggtctg gcggtttgtg
    11161 ccgtggtttt caccggaggc cttgtggtaa gtgtgcccgg ttgacgttgc acgggtcccg
    11221 gtttgccact cttctttttg cccttaccct ggaatgaacc taccagggca atgatcccca
    11281 gtagtatata aaaaatcagg ttgccaacat cttccatctg cttcagtatt tttttacctt
    11341 gtgttgcttg attttaggct gttttttcgg attaatcgtc tttgccttct tcttttgctg
    11401 cttcagcacc ggctgcttcg agggatcctt ggtcttcggc ttcggtccgg cacggtcagg
    11461 caccggctgc tgtggcttct tcagttccgg cttcgtctgc tccctgcgct ctttctgcct
    11521 tttaaccttg tacctgtcag cagtgttcct gctgttgttc tccatccgca tcttcgtctc
    11581 ctcgctctgg atctgataat gcctctccct cttctcctta agggctttct catcctcctt
    11641 ttcccgcctg gcctccttcc tggcctgttc cttcatcgct tttgcagccg ctctgctctc
    11701 accgctctcc tttacaggct tgctgcgacg cgatttgccg aaggtctccc tctcaaaccg
    11761 cttggtggca ttcttctggg cattgcactc agttgcctcc accacgaaaa gcagaacgat
    11821 taccgctgct acaaacctta ataatactaa ccctctcttc atttcgtttt tccgttagcc
    11881 gtgtcagact taaaataact acatttacag aaacggaagc aagcccatgt cagtttcaaa
    11941 tttaaggatt tttttactct tttctctgtt cacactgctt ataatctcct gtgacaggga
    12001 taagaacgat gtgatccctg acatcacggt tgacttcacc atcacttctt ccgatcctga
    12061 attttttgat ctcttcaatg ctcccggcac cagcgctctg atatcttcag cccacaggca
    12121 ccttggtctc ggtacagccg gttttgacgg caacggcata atagtctaca gtaccgggct
    12181 gcatggtttt gaattccctg cctttgaccg cacatgtccg cattgttacg ccaacaatga
    12241 agtgagtgtg gctgtgactg ttgacggcgt gtacgccatt tgcccggaat gttctacgaa
    12301 ttatgcactc ggagggtcag gcctgccgca gagcggaccc ggtcactatt atctcaagaa
    12361 ttaccgcaca tccttctccg gctacagtat aagggtctgg aacaggagat agaccctcct
    12421 agtacctgta atggtcaggc ttgtaggggc ccccttcggg aacgcctatg tagtctgact
    12481 gggccctggt cattttggtc aggctcacgc caagctgtgc aagatggaga cgtgccacct
    12541 cctcatcgag ttgcttggga agcctgtaaa caccgatagc gtgctctttg gtccacagtt
    12601 caatctgtgc cagcgtctgg ttgctgaatg agttgctcat cacgaacgac ggatggcccg
    12661 tggcgcagcc aagatttacc agcctgccct cggcaagaag gaaaattgca tggccgtcag
    12721 ggaacagata cttgtcaacc tgtggcttga tattgatctt cctgataccc ttgtatgcat
    12781 tcagcaaatc aacctgtatt tcgttgtcaa agtgtcctat gttgcagacg atggcctgat
    12841 ccttcatctt ctccatgtgt tcaatggtga tgacatcacg gttgcctgtt gcagtaacga
    12901 atatgttgcc ttcagcaagg ctctcctcaa ccgtccttac ctcaaagcct tccattgcag
    12961 cctgcagtgc acagatagga tcgatctcgg tcacaatgac ccttgcgcca tacgacctca
    13021 ttgaccttgc agatcccttg ccgacgtcac cgtatccgca tacgacaaca accttgcctg
    13081 ctatcatcac atccgttgcc ctcttgatac cgtcggcaag tgactcacgg caaccataaa
    13141 ggttgtcaaa tttgcttttt gttaccgaat cattgacatt gattgcaggc accaggagtt
    13201 ctcccgcctc cagcatctgg taaagacgat gcacgcctgt ggttgtctct tcggatatac
    13261 ccttcatctc actgaccgtc ctgtgccatt tctgcggatc ctcctccagt atcc
//
LOCUS       DAOPBK010000061        13306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4651_length_13306_cov_4.933137, whole genome shotgun sequence.
ACCESSION   DAOPBK010000061 DAOPBK010000000
VERSION     DAOPBK010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4651_length_13306_cov_4.933137"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..672)
                     /locus_tag="PLA17_05065"
     CDS             complement(184..672)
                     /locus_tag="PLA17_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPK84817.1"
                     /translation="MSFNKVILVGNVGKDPEIRRFENNIKASFSLATSETYTPRGGEK
                     VTQTEWHNIVAWRRLAELSENYIRKGSQILVEGKLRYRSYDDREGIKRYIVEVEADVI
                     QLLGRKPDNAGQPGQSYKSNQGYQGSTPGYSAPTQQASPSAGEEPAEDLSSQEPIDDL
                     PF"
     gene            945..1232
                     /locus_tag="PLA17_05070"
     CDS             945..1232
                     /locus_tag="PLA17_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPK84818.1"
                     /translation="MTKADIVNEIAKSTGIEKVVVQKTVESFMEAVKNSMVAGNNVYL
                     RGFGSFIVKKRAKKTARNISKNTTIIIPAHYIPAFKPAKSFINKVKTHVKK"
     gene            1433..2989
                     /locus_tag="PLA17_05075"
     CDS             1433..2989
                     /locus_tag="PLA17_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPK84819.1"
                     /translation="MNKDLIISVNDAEISIALLEDKQLVELNKEKQNVKFSVGDIYLG
                     KVKKIMPGLRAAFINVGFEKDAFLHYHDLGAQFRTLHRYYHLATQNKARMTSVSRFKP
                     EPDIDKDGQIADVLTVGQTVIVQITKEPISTKGPRLASEVSLAGRNLVLMPFSDKVSV
                     SSKIENADERNRLKLLIQSIKPKNYGVIVRTAAEGKKVAALDAELKELINRWEKALSG
                     VRRDVKSPRLLLGEMNRTTTILRDILSTDFNSIIVDNEVMAGEVKSYIREIAPEKEKI
                     VKVYKEQIPIFDYYGISRQIKGLFGRTVSCKGGSYLIIEHTEALHVIDVNSGNRSKSG
                     SDQESNALAVNLEAAREIARQLRLRDMGGIIVIDFIDMQSNENRQSLHERMKELMSAD
                     RAKHNILPLSKFGLMQMTRQRVRPEMTIVTDEKCPVCRGEGKTAPAILLTDEIERTLS
                     CIIEKVKTTRFILRTHPFVAAYLREGLFSRISRINKKYGVSIKVQGIDSFHFLQYQFY
                     DRQGNEIDLT"
     gene            complement(3051..4142)
                     /locus_tag="PLA17_05080"
     CDS             complement(3051..4142)
                     /locus_tag="PLA17_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPK84820.1"
                     /translation="MNYRTILITAILLPAGLALAGQQYSDIKTLKRSFTAGSEAILEV
                     TNKYGDIHVTHTDDDSVTVMVEITASSNTGDKIDALMSDVDVNIWQSGSVVRAETTFR
                     KGITPLFESLRGLTKNLINFNSRLKIDYRIECPPETVLSITNRYGDVYIGDETQVLAL
                     KLSNGNLDAATVGNAQMMELTFCKANVRSISEGKLILSFSELRTRETGKIKLTSTSSK
                     VWIDNCRSVDLDSKRDDLHFGSVESMTGVSYFSDIITDRVTGEINMTVKYGNLSFENV
                     DRGFRLTDIRSAYTDIDMALEKGSAYDLEIRHANAFVSLPGFDPEPERTEIDAEEKVF
                     LTKASAGSGAGRSKIRIDATRGEIRLLQK"
     gene            complement(4132..4731)
                     /locus_tag="PLA17_05085"
     CDS             complement(4132..4731)
                     /locus_tag="PLA17_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84821.1"
                     /translation="MDELEKHIRSIRDELDIHEPGHDLWDMIGRDLPGNEKMPETSII
                     RQKGSGRNRQKGFTGRRMQIRSVIWRAAVAVIVAGFAIATIAGMLLRSQRLNDPQVAA
                     VRETYRYYDERIRSLYEEAEPLLTANPDISTELNLGMTELESLSAQIIRDLDDNIASS
                     EVIEALIGNYRLRIELLEDMLNLMKEEGVEAEKNKGNEL"
     gene            complement(4724..5278)
                     /locus_tag="PLA17_05090"
     CDS             complement(4724..5278)
                     /locus_tag="PLA17_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK84822.1"
                     /translation="MATPDIHIHDRLIDECREGNRRAQFRLYELYSKAMFNTAYRIMG
                     NREDAEDMLQEAFTDCFRKIGSYRTESTFGAWLKTIVINRCISRLRKREAELVFIDDY
                     SRHEMQQDEPQEMIWPDPSVIARAVERLPDGYRVVFSLYLLEGYDHTEISQILGISES
                     TSKTQYSRAKEKLKKILSEMRRDG"
     gene            5854..7074
                     /locus_tag="PLA17_05095"
     CDS             5854..7074
                     /locus_tag="PLA17_05095"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPK84823.1"
                     /translation="MRTKFLLMTLCLFAVSCKLNEEQVSRKAERIHDNILSVDTHCDT
                     PMRLLRGSFDLGARNDRSCVDFPKMKEGRLDAEFFAIFIGQGPRTPEDYEREHGRTLE
                     VFDSIKASVARNQDIAGIALNPDDALRLKNEGKVAVFIGIENGYPIGTDITRIGQYYD
                     LGARYITLCHSSNNDICDSSSDKGGPEHNGLSAFGEEVVREMNRVGMMVDVSHISDES
                     FYDVLAVTKAPVIASHSSCRALCENPRNLNDDMLQALKKNGGVIQICILSDYLRQPVP
                     NPEYDAKVRELRESWRSMGELTMEQQEKRWEEFSKLKAQYVKLATIEDAVNHIQHVID
                     VIGIDHVGIGTDFDGGGSIEGCTDASMMKNITKEMIRRGYSRDDIARIWGGNIMRVMR
                     EVQAAASREQSVTN"
     gene            complement(7251..8342)
                     /locus_tag="PLA17_05100"
     CDS             complement(7251..8342)
                     /locus_tag="PLA17_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPK84824.1"
                     /translation="MKNKDYIISGIQQMGVGVYDLSEAWDWYIRVFGMDCRIFEDDTV
                     AKLMLPYTGGEPRSRHAVLAMNLQSGGGFEVWQYKGREPVYRDCETKLGDLGIFACKM
                     KVKDVDSAWRYWKDNNVEVIGQPAADPSGSKSFFVKDPFGNLFHIVGASDWFMNLKKI
                     SGGSYGAIIGVSDIDRALPLYSDILGYDRVVFDSTGTFEDLASVPGGNGRFRRILLAP
                     SKPFHGGFNPVYGQSVIELVQALDREPTKIFEGRLWGDPGFIHLCYDISGMDNLRKYC
                     SEAGFPFTVDSKASHEGSSFDMGEAAGFFAYIEDPDGTLIEFVETHKVPILKKYGWYF
                     DLRKRDPNKPLPSWMIRALRFSRVKSSRH"
     gene            complement(8353..9153)
                     /locus_tag="PLA17_05105"
     CDS             complement(8353..9153)
                     /locus_tag="PLA17_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK84825.1"
                     /translation="MKDFFAGKVVWITGASSGIGEALVKAFTRGGSRVIASSIDTEGL
                     ERVAGECSEMKGSITCVPFDLSDTSGIESLVKEVAQKEGRIDMLLNIGGISQRALLIE
                     TPMWLDRKIMEINYFGTIALTKALLPHMIAGGGGHIAATSSITGRFGFPLRSAYSASK
                     QALHGFFETLLIEHGKDNIRVSVLIPGRVQTAISLHALTANGREHGRMDDGQKGGVTP
                     EKAAKQIIRGLQRNKREILVGSKELLMLKIRKYLPSLFFRIAGRIKPM"
     gene            complement(9156..10484)
                     /locus_tag="PLA17_05110"
     CDS             complement(9156..10484)
                     /locus_tag="PLA17_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPK84826.1"
                     /translation="MGKKNIEKYDILYQLLKQYVDFWHNYIFYRRVIVLGRDAIDFSV
                     PTILAPNHQNALMDAMAVLCTLDRQLVFLARADIFRKKFVAKILYFLKILPVYRIRDG
                     FDAVKQNDDTFRTTLRVMENRNGVVILPEGNHATFRKLRQLKKGICRIAFQTAEAKKF
                     KGEINLVPVGLEYSHYWRFRQVLTVVYGNPIHVSEYYDSYRENPNRAITELRDRLSDE
                     MKKVMVHIEDEENYEAVNELRSIINEKYSDKIKYPKLFRDKRLISKLNELRLSDRESF
                     REICEKSLAVRDNATALNVTYRHLRKKSHRLLWLILSSLLLLVTSPIFVAGSLFNILT
                     FQVAYQQAIKSKDPAFISTARYGFFLGLSFIFGPLYLILALIFIKPWWLAIMVFMIIP
                     VLGIMAWNWFLLLLRTVGGFRIWNLRRTGNPLFKSLLNTYNQLVTHTLSL"
     gene            complement(10688..11065)
                     /locus_tag="PLA17_05115"
     CDS             complement(10688..11065)
                     /locus_tag="PLA17_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPK84827.1"
                     /translation="MKAEKGERIDKFLWFVRLYKSRSIATEECRKGRVIVSGHPAKPA
                     SLIAPGATITVRKPPVTYTYTVIGIPRGRIGATLVSSFITDITPEEEKAKLSQGRMVS
                     GYRPRGSGRPTKRERRELDNFLE"
     gene            complement(11069..11677)
                     /gene="pth"
                     /locus_tag="PLA17_05120"
     CDS             complement(11069..11677)
                     /gene="pth"
                     /locus_tag="PLA17_05120"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPK84828.1"
                     /translation="MKHLIAGLGNIGDEYRNTRHNVGFMVLDAAAAASGIIFKDSRYG
                     YVTRMRHKNAELVLLKPSTFMNRSGNAVRYWLKKEKIEIENLLVITDDLAIPTGSIRL
                     RSAGSAGGHNGLTSISEVLGTDQYARLRIGIGSDFPRGRQVDYVLGEWSPEELVIMKK
                     RIPLAVEMMLSFAVAGCELTMTAYNHKGKDLSDDSVTPAARK"
     gene            complement(11695..12315)
                     /locus_tag="PLA17_05125"
     CDS             complement(11695..12315)
                     /locus_tag="PLA17_05125"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPK84829.1"
                     /translation="MKTIEIKAFPREHLGKKSTNSLRAEDNVPCVMYMEKENLHFYAH
                     ENTFRGLVYTPDVFLVNLEVAGNSYKAVVKEIQFHPVTDRLQHIDFMQVHEEKPVTIN
                     IPIRITGESSGVKAGGKLRIKRRTLKVKGLIKNLPDHLTIDITGLGIGQSIKIGDLKY
                     DSLDVIDNKRAMIVSVEASRVSLKEEETAAPEAEDSAAEADAPDNK"
     gene            complement(12420..>13306)
                     /locus_tag="PLA17_05130"
     CDS             complement(12420..>13306)
                     /locus_tag="PLA17_05130"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPK84830.1"
                     /translation="AEKIAKHLGIELGKSSLLSFSDGEFQPCIDESVRGCHVFIIQST
                     NPPAENLMELLLMIDAAKRASAYKVNAVIPYYGYARQDRKDRPRVSLGAKLTANLLTK
                     AGVDRIITMDLHADQIQGFFDVPVDHLFGSALFAPYISNLNLPNLTVSAPDMGGSKRA
                     NAYARHLQCEMVLCYKLRKKPNVVEEISIIGDVEGRNVVIIDDMVDTAGTMTFAATVM
                     KDRGALSVRAVSTHPVLSGNAVEKIEKSPLEELVVTDTIPLVCQSPKIKVLSIDEIFA
                     ETIKAVYTNTSISSGFVF"
BASE COUNT         3290 a         3314 c         3195 g         3507 t
ORIGIN      
        1 ccgggggtaa gggagaagta tgccacctcg gaaccggaga tgaatgcaga tgctataata
       61 aggagaataa ggaagataat ttccgtgccc agggccaggg gtgatcctgt agcaacggcc
      121 agagccatta ttccggttaa tgaacctgcg ggaagtgtct ccaaaattat ccgggttggt
      181 tacctagaat ggcaggtcat caataggttc ctgagagctg aggtcttctg ccggttcttc
      241 tcctgctgaa ggagatgcct gctgagtagg ggcagaataa ccgggagtac tgccctggta
      301 tccctggttg ctcttgtaac tttggccagg ctggccagcg ttgtcaggct tgcggccaag
      361 aagctgtatc acgtctgctt caacctccac aatatatctc ttgatgcctt cgcggtcatc
      421 gtatgacctg tagcgaagct ttccctccac tagaatctgt gatcctttgc gaatgtagtt
      481 ttcagacagt tcggcaagtc ttctccaagc cactatgttg tgccattcgg tttgtgtcac
      541 cttttcacct cccctgggag tataggtttc gcttgtcgcg agtgaaaatg atgccttgat
      601 gttgttctcg aaacgccgga tctcggggtc cttgccgacg ttgcccacca gtattacctt
      661 attgaatgac atagctttat tatttattta cttatatgaa gtgatgtaaa gttacgaaaa
      721 atattgctgt gacgtcatca gggggctctc tctctggcgg gagccgtctc acgagcctgc
      781 ccgcagggca tgtttttgca ctgcatattc tattaggtat caaaaaatca gcaatttcgg
      841 actgaaaaaa accaatgtca actgtaaatg ataaataata ttttatattt gcacattgaa
      901 aacagaacat caaaataata tgttgaattt aaaattctta caacatgaca aaagcagaca
      961 ttgttaacga aattgccaag agcactggaa ttgagaaagt ggttgttcag aagaccgttg
     1021 agtctttcat ggaagcagta aaaaactcga tggttgccgg caataatgtt tatctcaggg
     1081 gatttggcag cttcatcgtg aagaaacgtg ccaagaaaac agccaggaac atatcgaaga
     1141 atacaaccat tatcattcct gctcactata tcccggcttt caaacctgcc aaatctttta
     1201 tcaacaaggt aaaaacacac gttaagaaat aatgatatgc caagcggtaa aaagagaaaa
     1261 aggcataaga tggcaactca caagcgtaaa aaacgcttgc gtaaagacag gcataagaag
     1321 aagaagtagc ataaatgcat agtctgtagc tcttatatct aacctaaaga tcagggatct
     1381 ttaggttagt tgcattataa aaatgctatt ggctggcaac aaattccgga atgtgaacaa
     1441 ggatttaatt atcagcgtaa atgatgctga gatatccatt gcactactgg aggataagca
     1501 gcttgtcgag ttaaacaagg aaaagcagaa tgtaaaattc tcagtcggcg acatttacct
     1561 gggtaaggta aagaagatta tgccggggct tagagcagcg ttcattaacg ttgggttcga
     1621 aaaagatgct ttcctgcatt atcatgacct gggagcccag ttcaggaccc tccaccgtta
     1681 ttaccatctg gctacacaga acaaggccag aatgacgtca gtgtcgaggt tcaagcctga
     1741 accagacatt gacaaggacg ggcagatagc tgatgtactg acagtaggac agacagttat
     1801 cgtgcagata accaaggaac ccatctccac aaaggggccc aggctggcct cagaggtgag
     1861 cttagcagga cgaaaccttg tcctcatgcc attctcagac aaggtctctg tctcttcaaa
     1921 gatagagaat gcagatgaac gaaatcgtct caagctcctg atacagagca taaagccaaa
     1981 gaattacggc gttatcgtgc gcactgccgc agaagggaaa aaggtagcag cactcgacgc
     2041 tgaactaaag gaacttatta accgatggga aaaggctctc tccggagtgc gccgcgacgt
     2101 caagtcgccg cgactgctcc tgggtgagat gaatcgcacg acaacaatcc tcagggatat
     2161 actcagtact gacttcaaca gcatcatcgt agataatgag gtgatggccg gcgaagtgaa
     2221 atcatacatc agggagatag ctcctgagaa agaaaagatc gttaaggtat acaaggagca
     2281 aatccccatc tttgactatt acggcatcag ccgtcagatc aagggtctct tcggacgaac
     2341 cgtttcttgc aagggcggct catatcttat cattgagcat actgaagcac tccacgtcat
     2401 agacgtcaac agcggcaaca ggtcaaaatc aggctctgac caggagtcaa acgccctggc
     2461 cgtcaatctt gaggctgcaa gggaaatagc ccgtcagctg cgcctgaggg acatgggcgg
     2521 aattatcgtg attgacttca ttgacatgca gtcaaacgaa aaccgccagt ccctgcatga
     2581 aaggatgaaa gagcttatga gtgcagacag ggccaaacat aatatactgc ctctgagtaa
     2641 attcggactg atgcagatga ccaggcagag ggtgcgcccg gaaatgacca tcgttactga
     2701 tgagaaatgc cctgtctgca ggggagaggg gaaaacagcc ccggctatac tgcttaccga
     2761 tgagatcgaa cgaacactct cttgtatcat tgagaaggtg aaaaccacaa gatttatcct
     2821 caggactcat ccgttcgtgg ctgcctacct gagggaaggg ctcttctcca ggatcagcag
     2881 gataaacaag aagtacggtg tcagcattaa ggtgcaggga atagattctt tccatttcct
     2941 gcagtaccag ttctacgaca ggcagggtaa cgaaattgac cttacctgat tttcctggta
     3001 tattgaatat tatgaataca tattcgtaac gacaggtctc agacctgtcg ttacttctgc
     3061 agcaacctta tctcgccccg ggtggcgtct atcctgatct ttgaccggcc ggcaccgctg
     3121 ccagccgagg cctttgtcag aaaaaccttc tcctcagcat ctatctctgt cctctccggt
     3181 tccggatcaa atcccggcag ggagacgaat gcattggcat gtcttatctc caggtcatag
     3241 gcagaaccct tttccagggc catgtcaatg tcagtatagg cagagcgaat gtctgtcagt
     3301 ctgaacccac ggtcaacatt ctcaaatgag aggttcccgt atttcactgt catatttatc
     3361 tccccggtta ccctgtcagt tattatatcg ctgaaatagc tgacacctgt cattgactcc
     3421 acggagccga agtgaagatc atcacgcttg gagtcaaggt caaccgaacg gcagttgtca
     3481 atccatacct ttgatgaggt ggaggtgagc ttgattttcc ctgtttcgcg ggtgcgcaac
     3541 tcgctgaatg agagaatcag tttgccctca ctgatacttc ttacgttggc tttgcagaag
     3601 gtgagctcca tcatctgtgc attgcctacg gttgcggcat ccaggttgcc atttgacagt
     3661 ttaagtgcca gaacctgtgt ctcatccccg atgtatacat caccgtaacg gttggtaata
     3721 ctgagcacgg tttccggtgg acactcaatg cgatagtcta tcttgagacg tgaattgaag
     3781 tttataaggt tctttgtcag gcccctgaga ctctcaaaga ggggtgtgat ccccttgcgg
     3841 aatgttgtct ccgcccgcac aaccgaacct gactgccaga tatttacatc aacgtctgac
     3901 atcagcgcat caattttgtc gccggtattt gacgaggcgg tgatttcaac catcaccgtg
     3961 acagagtcat cgtctgtatg ggtgacatgt atgtcgccat atttgttggt tacctcgagg
     4021 atagcctcgc tgcctgccgt gaaggatctc ttcagggtct tgatatcaga atactgctgg
     4081 cccgcaagtg ccagtccggc cggcagcagt attgccgtta ttaagatagt tctatagttc
     4141 attgcctttg tttttttctg cttcaacgcc ctcctccttc atcaggttga gcatatcttc
     4201 aaggagttct atccggagcc tgtagtttcc tatcagggct tcaatcacct cactgctggc
     4261 aatattgtca tccaggtcac ggatgatctg tgccgaaagg ctttcaagct cagtcattcc
     4321 caggttcagt tcggtgctga tatccgggtt ggctgtcagc agaggctctg cttcctcgta
     4381 aagtgatctt atcctttcgt cataatacct gtaggtctct cttacggctg ccacctgcgg
     4441 gtcattcagt ctctgagacc tgagcaacat gccagctatg gtggctattg caaatccggc
     4501 aactatcact gccactgcgg cacgccagat cacactgcgt atctgcatcc gcctgcccgt
     4561 aaaacctttc tgcctgtttc ggccgctgcc tttctgcctt ataatggagg tttcaggcat
     4621 cttctcattg ccgggaaggt cacgcccgat catatcccac aggtcatggc cgggctcatg
     4681 tatatcgagc tcatccctga tgctccttat atgtttctca agctcatcca tctcttcgca
     4741 tttctgagag tatcttttta agtttttcct ttgctcttga atattgtgtt ttggaggttg
     4801 attccgagat ccccagtatc tgtgagatct cagtatgatc atatccctca aggaggtaga
     4861 gactgaaaac cactctgtac ccgtcaggca gccgttcgac agccctggca atcacggagg
     4921 ggtccggcca tatcatctcc tgtggctcat cctgctgcat ttcatgcctg ctgtagtcat
     4981 caatgaaaac cagctcagcc tccctcttcc gcagcctgct gatacacctg tttatgacta
     5041 ttgtcttcag ccatgccccg aaggttgact cagtgcggta tgaccctatc ttcctgaaac
     5101 agtctgtaaa cgcttcctgc agcatatcct cggcatcctc cctgttgccc attatcctgt
     5161 aggctgtatt gaacatcgcc ttcgagtaaa gttcatacag cctgaactgt gctctccggt
     5221 ttccttcgcg gcattcatca atgagcctgt catgtatgtg tatatccggt gtggccaagt
     5281 tccagtttgt tcttgatcaa tgacgcaaca aagttacact ggttgcatca acattgcata
     5341 atcggttttc agggtcgcta tttttgcaat tatatgcgag cagcagcaca ttttaattat
     5401 atttgtgtct ggttaataac ctgatcagac aagaacaaga cagatcgtaa gatatttaag
     5461 atgcttttta ctgtccaaaa gcaatttttt cattcttttc atggctgccc cgacacttag
     5521 tcggggcttt ttttcatgag gcaccggttg tgccccacta agtgccgggg cttcttttat
     5581 gtgaggcacc ggcagcaaat ggacttctga cccggggttt cagcaatcag ctgactcaaa
     5641 aatacctgcc accgggttca ttggcttgat gtaggcaaaa acctttgatt catacctttc
     5701 ggttcagaaa acccttcgac tgactgcatg caggcacccc cgctctcatt cataatcaca
     5761 tatttaacct tttttatgac tttagctgca gcccggaagt tataatcaac ctttgatatt
     5821 catatcttag cactgacatc ataaattaat accatgcgta ccaaattcct tttaatgacg
     5881 ctctgccttt tcgcggtgtc atgcaagctt aatgaagagc aggtcagccg caaggctgag
     5941 aggattcatg ataacatcct ttctgttgac acacactgtg acacacctat gcggctctta
     6001 aggggcagtt ttgacctcgg agcgcgtaat gataggagct gtgtcgattt tccgaagatg
     6061 aaggagggaa ggcttgatgc cgaattcttt gccattttca tcggacaggg gccgcgtacc
     6121 cccgaggatt acgagaggga gcacggaagg acccttgaag tatttgattc aatcaaggca
     6181 agcgtagccc gcaaccagga tatcgccgga atagcactta atcctgatga tgccctgcgg
     6241 ctgaagaatg aaggcaaggt ggcggtgttc attggaatag aaaatggcta tccgataggt
     6301 acagacatca caagaatcgg gcagtattat gacctcggtg cgcggtatat cacactgtgc
     6361 cattcttcca acaacgacat atgtgactcg tcgagtgaca agggtggccc tgaacataat
     6421 ggtctttccg ccttcggcga agaggtggtc agggaaatga accgcgtagg gatgatggtt
     6481 gacgtttcac acatctctga cgaatcattt tatgatgtac tggccgttac gaaggcgcct
     6541 gttatagctt cacattcatc atgccgcgca ctgtgtgaga atccgcggaa tctgaatgat
     6601 gacatgcttc aggccctgaa gaaaaacggg ggcgttattc agatatgcat actgagcgac
     6661 tatctcaggc agccggtacc aaacccggag tatgatgcaa aggtcaggga actgcgcgaa
     6721 agttggagga gcatgggtga gttgaccatg gagcagcagg aaaaacgctg ggaggagttc
     6781 tcaaagctga aggcacagta tgttaaactg gctaccatag aagatgccgt gaaccatatt
     6841 cagcatgtga ttgacgtcat cgggattgac catgtgggca taggaactga tttcgacgga
     6901 ggaggcagca tagaggggtg tactgatgcc tccatgatga agaatatcac gaaggagatg
     6961 atccgccgcg gctacagccg tgatgatatt gccaggatat ggggcggaaa tatcatgagg
     7021 gtcatgaggg aagtgcaggc agcagccagc agggagcaga gcgtgaccaa ctgatccggc
     7081 ggttcatttc agtgaaggcg ccggactaca gcgaagacac cggatttcag cgaaagagcc
     7141 ggagtgtagt gaagacgccg gagtgcagcg aagacaccgg atttcagcga aggcgccgga
     7201 ctacaatgaa gagaatagtg tgacaatgac agcctgcagg gcttaaagca tcaatgcctg
     7261 ctgcttttga cccgcgagaa tctcaatgcc ctgatcatcc atgacggcag tggcttgttg
     7321 ggatcgcgtt tccgcaaatc aaaataccat ccatactttt tgagtatggg cactttgtgt
     7381 gtctccacga attctataag ggttccgtca ggatcttcga tataggcgaa gaagcctgct
     7441 gcctcgccca tgtcaaagct gctgccctca tgactcgcct tgctgtcaac cgtaaacggg
     7501 aagcctgcct cgctgcaata cttgcgcagg ttatccattc cgctgatgtc atagcagagg
     7561 tgaatgaatc cggggtcgcc ccagagcctc ccttcaaata tcttcgtcgg ttcgcggtca
     7621 agcgcctgta ccagctcaat gacactctgg ccgtagacgg gattgaatcc gccatggaat
     7681 ggttttgagg gcgccagaag gatgcgtctg aagcgtccat tgccacctgg caccgaagcc
     7741 aggtcttcaa aagtgcctgt gctatcgaac accaccctgt catatcccag tatgtcagaa
     7801 tagagcggca gggcacggtc aatatctgat acgccgataa tggctccgta tgatccgccg
     7861 gatatctttt tcagattcat aaaccagtcg gatgctccca cgatgtggaa caggttgccg
     7921 aacgggtctt tgacaaagaa gctcttactg cctgaaggat cagccgctgg ctggccaatc
     7981 acctccacat tgttgtcttt ccagtatctc caggcgctgt caacatcctt caccttcatc
     8041 ttacaggcga agattccaag gtcgcccagt ttggtttcac aatcacggta taccggctct
     8101 cttcccttgt actgccacac ctcgaatccg cccccgctct gcaggttcat cgccagtacg
     8161 gcatgcctgc ttcgcggttc gccgccggta tatggaagca taagtttcgc caccgtatca
     8221 tcctcgaaga tgcgacaatc cataccgaac accctgatgt accaatccca tgcttcactg
     8281 aggtcataaa cccctacgcc catctgttgt ataccgctga tgatgtaatc tttatttttc
     8341 atgattcctt ccttacattg gtttgatccg tcccgctatc ctgaagaaaa gcgatggtaa
     8401 atatttcctt attttaagca tcagaagctc cttgctgcca acaagtatct cccttttatt
     8461 ccgctgaagc cccctgataa tctgctttgc agctttttcg ggggtgacgc cgcctttctg
     8521 cccgtcgtcc atcctgccgt gttcccttcc gttggctgtc agcgcatgaa gcgaaatggc
     8581 agtctgtacc cttcccggta ttaacacgga gacccggatg ttgtccttgc cgtgttcaat
     8641 gagaagggtc tcgaagaaac cgtgcagcgc ctgcttggag gctgagtatg ccgagcgcag
     8701 cgggaagccg aaccggcctg tgattgagct ggtggcagca atatgccccc ctcctccggc
     8761 aatcatgtgg ggcaggagtg ccttggtgag ggcgatggtg ccgaaatagt tgatctccat
     8821 gatcttacga tcaagccaca taggtgtctc tataagcaga gccctctggc ttatcccccc
     8881 gatattgagc agcatgtcaa tccggccttc cttctgagcc acctctttta ccaggctttc
     8941 gataccggag gtatctgaca ggtcaaaagg cacgcaggta atactcccct tcatttcgga
     9001 gcactctccg gctacccttt ccagcccttc tgtatcgatc gatgatgcta tcaccctgct
     9061 tccgccgcgg gtgaaagctt ttaccaatgc ttcccctatt cctgaggagg ccccggtaat
     9121 ccagactacc tttccggcaa aaaaatcttt cattatcaga gagataaggt gtgtgtgacg
     9181 agttgattat atgtgttgag caatgatttg aagagcgggt ttccggtgcg gcggaggttc
     9241 catattctga aaccgcccac ggtgcgaagc agcagcagaa accagttcca tgccattatg
     9301 cccagtaccg ggataatcat gaaaaccatt attgccagcc accacggctt gatgaagata
     9361 agggccagta tcaggtagag cggcccgaag ataaagctta gccccaggaa gaacccgtat
     9421 cttgccgtgc tgatgaatgc ggggtccttt gactttatag cctgctggta tgccacctgg
     9481 aatgtaagta tattgaagag ggagccggcg acaaatatag gagaggtgac aagcagcagc
     9541 agtgatgaca gtatcagcca taagagccgg tggcttttct ttctcaggtg acggtatgtg
     9601 acattcagcg ccgtggcgtt gtcacgcacc gcaagagatt tctcacatat ctccctgaag
     9661 ctctcccggt cagacagtct aagctcgttg agcttgctga tcagccgctt gtcacggaaa
     9721 agcttcgggt actttatctt gtcactgtac ttctcattga tgatgcttcg aagctcattg
     9781 acagcctcat agttctcctc atcctcaata tggaccataa ccttcttcat ctcatcagag
     9841 agcctgtcac ggagttcagt tatcgccctg ttgggattct cgcggtatga gtcatagtat
     9901 tcagatacat ggatcggatt gccgtacaca acagtaagca cctgcctgaa acgccagtaa
     9961 tgtgagtact caagcccgac cggaaccagg ttgatctcac ccttgaactt ctttgcctca
    10021 gcagtctgga aggcgatgcg gcagataccc ttcttcagct gtctcagctt cctgaaggtg
    10081 gcatgattgc cttcgggcag gataaccact ccgttgcggt tttccatcac cctcagtgtg
    10141 gtacggaagg tatcgtcatt ctgctttact gcatcaaagc cgtccctgat acggtagaca
    10201 ggcagtatct tcaggaaata gaggattttt gccacgaact tcttcctgaa gatatcagcc
    10261 ctcgccagga agaccagctg acggtcaagg gtgcatagca cagccatggc atccatcagt
    10321 gcattctggt gattgggcgc caggatagtt ggcacggaaa aatctatagc atccctgccc
    10381 agaacgataa ccctgcgata gaaaatataa ttgtgccaga aatcgacata ttgtttcaaa
    10441 agctgataga gaatatcata cttctcaatg ttcttcttac ccattcccca atgaagtttt
    10501 taccttctga aagatagttg tgaagcccac gggcctcaca ttttgctaat ttatgaaaag
    10561 tttatccgta cacttgataa attggcagga gtaatggatt attatctcct ttttaggtat
    10621 ttataccacc atggccggag gtgtgctgtt tcaatccatg aattaattcc ggagtgtgac
    10681 attgattcta ttccaggaag ttgtcaagtt ccctgcgttc cctcttggtc ggacggccgc
    10741 tgccgcgggg ccggtaaccg ctgaccattc ttccctggct aagcttcgct ttctcttctt
    10801 ccggggtgat gtcagttatg aagctgctta ccagcgtcgc acctatcctt ccccggggaa
    10861 tgccgatgac ggtgtaggtg tatgtgaccg gtggtttcct gacggtgatg gttgcgcccg
    10921 gggcgataag tgatgccggt ttggcgggat ggccgctgac aatgaccctt cccttcctgc
    10981 attcttcggt ggctatactg cgggatttat acagcctcac aaaccagagg aacttatcta
    11041 tcctttcacc cttctctgct ttcatagctc actttctggc agcaggggtg acgctgtcat
    11101 cactgagatc tttacccttg tggttatagg cggtcatagt gagctcgcat cctgccacgg
    11161 caaaagagag catcatctcc accgccagtg gtatcctttt cttcataatg acaagctctt
    11221 ccgggctcca ttcgccaagg acatagtcaa cctgcctgcc tctggggaag tcactcccga
    11281 tgcctatcct gaggcgtgca tactgatctg tcccaagcac ttcactgata cttgtcagtc
    11341 cgttgtgccc gcctgcgctt cccgctgacc tgagtcttat gctgccggtg ggtatggcca
    11401 gatcatctgt aataaccagt aggttttcaa tctcaatctt ttccttcttc agccagtacc
    11461 gcacggcatt gccgctgcgg ttcatgaatg tagagggctt gagcaacacc agttcagcgt
    11521 ttttgtgcct catcctggtg acatacccgt aacggctgtc cttaaaaatg ataccggatg
    11581 ctgcggctgc agcatccagt accataaatc ctacattatg acgcgtgttc ctgtactcgt
    11641 cacctatgtt gccaagaccg gcaataagat gtttcattac atacagttac cagcttattt
    11701 attgtcaggg gcatcagcct cggcagcaga atcttcagct tccggagcag ctgtttcttc
    11761 ctccttgagg gaaaccctgg aggcttcgac agatacaatc attgcgcgct tgttgtcaat
    11821 tacatccagg ctatcatatt tgagatcacc tattttgatc gactgtccga taccaaggcc
    11881 ggttatgtca atggtcagat gatcaggcaa gttctttatt agtcccttaa ccttgagagt
    11941 tctgcgcttg attcgaagct tgccgccggc cttcacacct gatgattcac cggtgatcct
    12001 gatcggaatg ttaattgtaa caggcttctc ttcatgtacc tgcatgaaat caatatgctg
    12061 cagcctgtca gtaacaggat ggaactggat ttccttcacc actgccttat agctatttcc
    12121 tgcaacttca aggttcacaa gaaacacatc gggagtgtag accagtccgc ggaaagtgtt
    12181 ctcgtgtgca taaaaatgaa gattttcctt ctccatgtac atcacacatg gtacattatc
    12241 ctctgcccgc aggctgtttg tactcttctt gcctaaatgt tcacggggga aggctttgat
    12301 ctcaatagtt ttcatcaatc tttaatttgt tgtgctactc aaggttaatg ccatataacc
    12361 tcccatcaca cgtttgacgc tttgttgaaa aaaatggcgt gcaaatatag ctaaaaagct
    12421 tagaaaacaa aacccgaact gatagaggta ttcgtataaa ccgcttttat tgtttcggcg
    12481 aagatctcgt caattgaaag caccttaatt ttcggcgact ggcagacgag tggaatagtg
    12541 tctgtgacaa cgagttcctc caatggagac ttctctatct tctcgaccgc atttccggaa
    12601 agtacggggt gtgttgatac agctcttaca ctgagagcac cgcggtcttt cataaccgtg
    12661 gcagcaaaag tcatcgtccc ggcagtgtct accatgtcat ctatgatgac cacattcctt
    12721 ccctccacat ctcctatgat tgaaatctcc tcaaccacgt tgggcttctt cctcagcttg
    12781 tagcacagca ccatctcaca ctgcaggtgg cgtgcatatg cattagccct ttttgagccg
    12841 cccatatcag gggctgaaac agtcaggttg gggagattaa ggttgctgat atatggagcg
    12901 aagagggctg aaccgaagag gtgatcaacg ggtacatcga agaacccctg tatctggtct
    12961 gcatgaagat ccatggtaat gatcctgtca acaccagcct tggtcagcag gttggcggtg
    13021 agctttgctc ccagggatac tctcggccgg tctttgcggt cttgccgtgc atagccgtag
    13081 tacggtatta ccgcattcac cttgtaggcc gaagccctct tggcagcgtc aatcatcagg
    13141 agcagctcca taaggttctc ggcgggaggg ttggtagact gaattatgaa cacgtgacaa
    13201 cccctgaccg attcgtcaat acagggttgg aattcgccgt cactgaaact cagcagtgag
    13261 ctcttgccta gttcgatgcc aaggtgtttg gcaatctttt ctgcta
//
LOCUS       DAOPBK010000062        13170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4698_length_13170_cov_5.763401, whole genome shotgun sequence.
ACCESSION   DAOPBK010000062 DAOPBK010000000
VERSION     DAOPBK010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4698_length_13170_cov_5.763401"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..747)
                     /locus_tag="PLA17_05135"
     CDS             complement(142..747)
                     /locus_tag="PLA17_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84831.1"
                     /translation="MKKLIISMILLAVGGALWSQENGSLNFSGSYIYMPQYRMSGAGY
                     TISLQQSLKPWLRLEIGGSYTLGSRTIKRNKTVNNVRLLDLDYHHAAYSLHVAPVVTT
                     GNAGVASLELFAGPVITWQSNVFDLNHYEMPESPGYIGRMTDIVYVNRVVEGTFFGGI
                     AGCRVNVRTGENWQVNLGVSAMGIIKAVSSVSASIGVRYCW"
     gene            complement(758..1630)
                     /locus_tag="PLA17_05140"
     CDS             complement(758..1630)
                     /locus_tag="PLA17_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84832.1"
                     /translation="MKAKNILLFLLAAALMASCEREDMTSPVALFSAQTPPIYKFKVN
                     LDASGSFPTEDVDWLEYRWDINGDHLGWETDWSGNPIVTIEFPFAISGYIGLQVRNSD
                     GRITELYQGFITDLNYEIFRPHTNLEIGFSVITYRFYHPGHLWAWMWSCENIQLPDSD
                     GWYNYTAAADRFAYGSLMPWSVADTLEKNYHLPSKADWQEMINYCGGATLAGFNLQVE
                     AEHGLQLTLPGIVTEGLLREQNSAGYYWTGDEVDEESAWALKISADSDEAKFVILDKS
                     SLASVRLMNILNYR"
     gene            complement(1641..2327)
                     /locus_tag="PLA17_05145"
     CDS             complement(1641..2327)
                     /locus_tag="PLA17_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84833.1"
                     /translation="MKITAYITLILTVSIAGTNCLAQTDTVLVETELKQDQEFYYRNK
                     YRYLDVRLGDEDRLLKIGIQPFKPSDNFSFLVFTLHGGFEQKMNTSLTTVTEINSVMT
                     WTKEEAFHQQGISLGGRYYFLKKREIEQGRSGNNCQGVYGLFKMSNLLALITLRADDE
                     NPDPMSRDPYLERYIKGSLTPETGIGYQQRLDYHLYFDSALTCNYNILDKDFGFQISF
                     LFGVLFSYNK"
     gene            complement(2332..3009)
                     /locus_tag="PLA17_05150"
     CDS             complement(2332..3009)
                     /locus_tag="PLA17_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84834.1"
                     /translation="MKTKDKLITRVLRRRQYLLCLTALVLALLTSREANGQDFRESPY
                     NKRIFSINSGSVFSGDGDCWGIGTGLSHLKTIGRRFYIRENLTSWIINGESWIEGAFE
                     NQTAIDLSAELGFSPFRMGQRFFSIHGGVCSAFFINSDPTNGGRWLVYFPDTGDYSYM
                     EYYEQVLIKTLDFGITFGVNYHRQITPALYLNARADFRTHFSTASAISMITVGIGFDA
                     RELFKKQ"
     gene            complement(3017..3874)
                     /locus_tag="PLA17_05155"
     CDS             complement(3017..3874)
                     /locus_tag="PLA17_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84835.1"
                     /translation="MKILHYIAMSLVLLGTCDLTAQEVMQKASRSDERSFGKGISGVS
                     VYGEKASITVRGAAVSDVKVLLRPVSRNRTRELAIADLKYIHYSAEKEGDILVIRNSF
                     RGKLEQITSNLSMEIEIIMPSSLPVSITNLYGPVEAGNLASITANVSFGSLRLKDISS
                     AARITARYSDMELTSVRGRADIKAEKSDILTTALGATTVINCSYGTANLEMSGSGPLI
                     VKGYRTTVNISVDEPGRFQYSLKAPQGRIIMSDGKQYRNQPVEVTPQSPAGLIDVTTS
                     YCDITIAKK"
     gene            complement(3871..4575)
                     /locus_tag="PLA17_05160"
     CDS             complement(3871..4575)
                     /locus_tag="PLA17_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84836.1"
                     /translation="MNREFDDILSERIRELPSHQPSAALWKEIESLLDREDAILSQLH
                     DLPIHHPSSAVWENIEAALQEERPDRSSDADFRSTVKRTLQMPPDSDDIMLTGKKRAG
                     IRRRTLLMTIGSVAAAALILFAIPQLLPTEKGITVESEIIPGEEYGSPVTMTGADEDP
                     IEVIRELCRTGAPICQSELFREKMQLYQELNEELRQLEMVIDQVGDSSEIIQSVIRIE
                     NLKSGTLQELIQLIHS"
     gene            complement(4559..5107)
                     /locus_tag="PLA17_05165"
     CDS             complement(4559..5107)
                     /locus_tag="PLA17_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK84837.1"
                     /translation="MQQSEQNQGDRELVRGCLGNNRLVQRALYEKYSARLYTLIYRLI
                     SDHDASNDVLQDAFIEIFRSLATFRFESSLFTWMRTIVIRNAMSYLRKNARLQVITDE
                     IPDQASESSFNFTAEHLEKAILSLPAQARSVFILIEVEGYKHQEVAEILGINEGTSKS
                     QLNYAKKLLRNRLTKPSDEQRV"
     gene            complement(5302..6159)
                     /locus_tag="PLA17_05170"
     CDS             complement(5302..6159)
                     /locus_tag="PLA17_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPK84838.1"
                     /translation="MEVVYLNGQFLPKDQALISPDDRGFLFGDSVYEVARWYGGYFFD
                     LPGHTGRFRRSMKETNMVWEDEAKLEEIFEELIERNGLGADCAIVYFQATRGVAPRNH
                     AFPDPPVKPTIYGFARRHNINTLAAERGVAIWLTPDPRWNRCDIKTTALIANVLPYQE
                     AHDQGFAEVCFVRNGFVTEGAHSNIFFVRNEALYTHPENNDILSGITRKNIISVARAN
                     DVSVVEEAVATDMIPYMNEAFICNTTGEIVPVTRISDQVIGDGNPGHLTRKLQVLLRE
                     FIQSCDPHR"
     gene            6347..7213
                     /gene="lipA"
                     /locus_tag="PLA17_05175"
     CDS             6347..7213
                     /gene="lipA"
                     /locus_tag="PLA17_05175"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPK84839.1"
                     /translation="MRGKRRLPSWLRMERASGESYTMVKNLVESHRLHTICTSGNCPN
                     IGECWNAGTATLMILGDICTRSCKFCGTKSGRPLPPDPGEPDRVAQAVKTMHLKHCVI
                     TSVDRDDLPDGGAAHWAETIRRIREVNPGVTIETLIPDFRGKTEHIDMVIDAGPDVIS
                     HNMETVRRLTPLIRSVAKYEVSLAVLRHIASRGVRAKSGIMLGLGESEGEVTETLADI
                     YATGCRIVTIGQYLAPSLEHIPVVEYVTPEKFEEYRKTGLETGFDFVESSPLVRSSFH
                     AEKHVKERLHDL"
     gene            7241..7945
                     /gene="lipB"
                     /locus_tag="PLA17_05180"
     CDS             7241..7945
                     /gene="lipB"
                     /locus_tag="PLA17_05180"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPK84840.1"
                     /translation="MINNAGIKVIFDDLGLRDFKETWDYQEHFFNLKVAEKGRPESDG
                     KTTPDRLIFVEHNHVYTLGKSGSEQNLLLDYIQLRARDASFYKIDRGGDITYHGPGQL
                     VGYPIFDLEKLKLGLREYIHLLEEAIIRCISRFGIEGSRLEGATGVWIDPDKGARARK
                     ICAIGVRASRFVTMHGFALNVNTDLSYFNHINPCGFTDKGVTSIGKETGEKREMDDVK
                     QVMYNTMREVFGYEYY"
     gene            7957..9663
                     /gene="recJ"
                     /locus_tag="PLA17_05185"
     CDS             7957..9663
                     /gene="recJ"
                     /locus_tag="PLA17_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPK84841.1"
                     /translation="MDKRWLLKERGDEHVVTTLATEMGVPVTVAGLMAQRGITTKQQA
                     EEFFNPTLESLHDPFLMKDMNRAVDRITTAISRNEKILVYGDYDVDGTTSVAMLYSFL
                     RERHSCLDYYIPDRYHEGYGLSSRGIDYAAGGGCKLIIALDCGIKAVEKVKYARSKGI
                     DIIICDHHLPGEGIPDATAVLDPKQPGCGYPYKEMSGCGVGFKLMQAICMLQGIPFEE
                     IIPYLDLVAVSIGSDIVPMTGENRILAYYGLKQLNESPRLGFQKIIEKAKIKKPLVIE
                     DVVFRIGPRINAAGRMESGSRAVDLLVSQNSQEAMTICDAIDVNNDHRRKKDSEITAE
                     ALRMVSDDIRSGNARTTVLFHRGWHKGVIGIVASRLIETYYRPTVILTESKDGYATGS
                     ARSVPGYDLYQAIEYCSDLLESFGGHMFAAGLTLKQENLQEFRERFEQYVSETIRDEH
                     LVPSVNIDEEIPLEEVTMEFCRHLEKFQPFGPENTAPVFVSRRVKTLSCQSVGANGIH
                     LKLSFARENGEPVGAIAFGQADMIETFRESGGVVDIAYSVEMNEFRGKRSVQLNIKDI
                     KG"
     gene            9801..10607
                     /locus_tag="PLA17_05190"
     CDS             9801..10607
                     /locus_tag="PLA17_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84842.1"
                     /translation="MMLRWNAVLMLLFFHFTVIHAGEPLFVPHGAGAMGVAFSVTATP
                     GHWNCFHNQALLTAVPSSGFSIGLENRFMMPALSSKAVSGVLAFKPAPLGIMVSHYGN
                     ADYFRIFTGLGSAVRISDGISLGLQVDYISENGAGEYRDVSFVTFETGVTCLVAPALT
                     LGVHLFNPFSPLNTLPSSIDAGFHWKQSDELFLTLTGSKVSNEPLSVQCGISWDVMKR
                     LTLRTGYMSSPSAFAFGIGFKTALLEADAGFLLNGVTGVTSSVSITWLIR"
     gene            10697..12760
                     /locus_tag="PLA17_05195"
     CDS             10697..12760
                     /locus_tag="PLA17_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84843.1"
                     /translation="MSFRSAVSVMILLLLSGTLFCQEENLSLRESVEEILALSDDADP
                     AALLEELEELKAFPVNINGGDEKEISRLFFLTEFQVMVLADHVRKNGSVVTLYELALL
                     PAFDRNTVLLMAPYISLKPAKPKGGRSYSRTLVTLTASARFSNDENAAEGMRSLMKMK
                     HKQGSFTFGLTAENDPGEPFIFKNAPGADFLSGYVQYEGKGLINRIIAGDFSVRAGEG
                     VLLNSSRWMGSWLSSPSFMSGRSVSSPYTSTEENSFFRGISLTLGGVNSGALLFASSN
                     KIDARLLFTDDSVATGVRNLVRGGIHVTESQLAARNSLTETVTGLRLIAGTGKARGGV
                     TATATWFSLPFVPDVTEPENLHAFAGERLLNLSADIRAGTGSILFFTEAGLGLSGPLT
                     GTAGTDNPGPQADTTGTDPPGSVSAIDRLNLRNSWAATAGLRAKPSGRVTFNLLARHF
                     APGYHAFHSGAFRAGSGSGNETGLSASVHLEAARHLFITAAADHYRIPWPRYRSSSPS
                     YGSRIEIKGEYVPRENISLRLSWTSSAREYDAAAETGTAVPVTRTRQQLGLVFDMSIA
                     STLRLTTRASASFIHPSQERGYLLGQDFSWSFRRVPLKLWFRYALCSTGGWDSRLYAW
                     ENDLLSSFTIPALYGEMARSFLMLSWKPAEFIEARVKYGYTDGRNNSSGGMKQELKGQ
                     IKIEF"
     gene            12888..>13170
                     /locus_tag="PLA17_05200"
     CDS             12888..>13170
                     /locus_tag="PLA17_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84844.1"
                     /translation="MIVTISNFKTEVDFSLDENMRRVITSEMGQHKIDSIKTREDLIC
                     YYKNLTPLFQPGYSSLSDDNHYIFAKVEYLLAQECFQDNCKSLTRREILE"
BASE COUNT         3099 a         3269 c         3434 g         3368 t
ORIGIN      
        1 ctgcgaacca gttcctgctc actcaaatgt ggtgtgctca tgctgtaaaa aatttctgca
       61 aaggtagcac ttgagagtca aaaggcaata gcggaaattc gctgcagggg atataacaat
      121 gtaaatatat acctgtgtct ctcaccagca gtatcgtact ccgatgctgg cactgacaga
      181 tgagactgcc ttgattatgc ccatggcact aaccccgagg ttcacctgcc agttttctcc
      241 ggtacgcaca ttgaccctgc accctgcgat acccccgaag aaggttcctt ccaccactct
      301 gttgacatat actatatctg tcatccttcc aatgtaacca ggactttcag gcatctcata
      361 gtggttcagg tcaaacacat ttgactgcca tgttattacc ggtccggcaa aaagttccag
      421 tgaagccacc ccggcgttgc ctgtagtcac aaccggagcc acatgcaaac tatatgctgc
      481 atgatggtag tcaaggtcga gcagcctcac gttatttaca gttttgttcc ttttgattgt
      541 gcgagagccg agggtatagc ttcccccgat ctcgagtctg agccatggct tcaggctttg
      601 ctgaagggat atggtataac cggcgccgct catccggtac tgtggcatat aaatatatga
      661 gcccgaaaaa ttgagtgatc cgttctcctg tgaccagagt gcaccgccaa cggcaagcag
      721 gatcattgat ataatcagct ttttcatggt ttccgggtta tctgtagttt aatatattca
      781 ttaatcgcac tgaagccagg cttgatttat caagtatgac aaactttgcc tcatcactgt
      841 cagccgagat cttaagggcc catgcagact cttcatcaac ctcatcgccg gtccagtaat
      901 aacctgcgct gttctgttcc cgcaacaggc cttcagtaac tatccccggc agggtgagtt
      961 gcagtccgtg ctccgcctct acctgcaggt tgaatcctgc cagggttgct ccgccacagt
     1021 agttgatcat ctcctgccag tcggcctttg agggaaggtg gtagtttttt tccagggtgt
     1081 ctgccacaga ccatggcatc aggctgccgt atgcgaaacg gtctgcagca gcagtatagt
     1141 tgtaccaccc gtctgagtca ggaagctgaa tgttctcaca agaccacatc catgcccaga
     1201 ggtgaccggg atggtagaac cggtaggtta ttactgagaa tccaatctca agattcgtat
     1261 ggggcctgaa aatctcgtag ttcaaatcgg taatgaaacc ctggtaaagt tccgtaattc
     1321 ttccatcgct gttcctcacc tgaagaccta tatagccgct gattgcaaaa gggaactcta
     1381 ttgtgacaat gggattgccg ctccagtctg tttcccatcc gaggtgatcg ccgttaatat
     1441 cccagcggta ttcgagccag tcaacatctt ctgtcggaaa ggagcctgac gcatcaagat
     1501 ttaccttgaa cttatatatc ggtggcgtct gagcgctgaa aagggctacc ggcgatgtca
     1561 tatcttcccg ttcacatgat gccatgagcg ctgcagccag caagaatagg agtatatttt
     1621 ttgctttcat ctcaggtagt ctatttgtta taactgaata aaactccgaa cagaaaactt
     1681 atctggaatc cgaaatcctt gtccagaata ttgtagttgc atgtaagagc cgaatcgaaa
     1741 tagagatggt agtcgagccg ctgctggtac ccgatgcctg tctccggagt gagcgagccc
     1801 ttgatgtacc gttcaaggta aggatcacgt gacattggat cagggttttc gtcatccgcc
     1861 ctgagggtta tcagggcgag caggttactc attttgaaga gcccgtaaac accctgacag
     1921 ttattgccgc tgcgtccctg ttcaatttct ctctttttga ggaagtagta ccggcctccc
     1981 agtgatatac cctgctggtg aaatgcctcc tctttggtcc aggtcatgac agaattgatc
     2041 tcggtcactg tggtgagtga cgtgttcatc ttctgctcaa agcctccatg cagtgtaaat
     2101 accaggaaac tgaaattgtc gctgggcttg aaaggctgga tgcctatctt cagcagcctg
     2161 tcttcatctc ccagtcgcac atcgaggtag cggtacttgt tgcggtagta gaattcctgg
     2221 tcctgcttca gttcggtctc aaccagtact gtatcggtct gtgcaaggca gttcgttcct
     2281 gcgatgctga ctgtaaggat aagagttatg tatgctgtta tcttcatctc tttactgttt
     2341 tttaaagagt tcgcgggcat cgaagccgat acctacagtg atcattgata tggccgaggc
     2401 ggtactgaaa tgtgtccgga aatcggcccg ggcattcagg tatagtgccg gagtaatctg
     2461 ccggtggtag ttgactccga aagtaattcc gaaatcaagt gtttttatca gcacctgctc
     2521 ataatactcc atgtagctgt aatcaccggt gtcagggaag tagacaagcc atcgtccgcc
     2581 gttggttgga tctgagttga taaaaaaggc actgcagaca ccaccgtgta tgctgaagaa
     2641 gcgctgaccc atcctgaagg gcgagaaacc cagttcggca ctcaggtcga tggcagtctg
     2701 gttctcgaag gccccttcaa tccagctctc tccgttgatt atccatgatg tcaggttctc
     2761 ccgtatatag aaccgcctgc caatggtctt caggtgagat aagccggtac cgattcccca
     2821 gcagtcgccg tcgcctgaaa agacagaccc gctgttaatg ctgaagatcc gcttattgta
     2881 aggactctcc ctgaaatcct gtccatttgc ctcccggctc gtcagcagag ccaggaccag
     2941 ggctgtcaga caaagcaaat attgccttct ccgtaagacc cgtgttatca gtttatcctt
     3001 tgttttcatg gccggattat ttctttgcta ttgtaatgtc acagtatgaa gtggttacat
     3061 caataagccc tgcaggagac tgtggtgtga cttccaccgg ctggttccgg tattgtttcc
     3121 cgtcagacat gattattctt ccctgcggtg ccttgagtga atactggaat ctgcccggtt
     3181 cgtcaactga gatgttaacc gtggtacggt accccttgac aatcagaggc cctgatcctg
     3241 acatctccag gtttgcagtc ccgtacgagc agttaatgac tgttgtggct ccgagggctg
     3301 tagttaatat gtcagatttt tctgctttga tatcagccct tccgcggaca gatgtcagtt
     3361 ccatatccga gtagcgggct gttatcctgg ctgccgagct gatatctttc agccggagcg
     3421 agccgaagga gacattggcg gttattgaag cgaggttacc ggcttcaacg gggccataca
     3481 ggttggtgat gctcaccggg agggaagaag gcatgatgat ctcgatctcc atgctgaggt
     3541 tactggttat ctgttccaat ttacccctga atgaattcct gatcaccagg atgtcaccct
     3601 ctttctcagc tgagtaatga atatatttca gatcggctat agccagctcc ctggtcctgt
     3661 tccgtgagac aggcctgagc agcaccttca cgtcgctgac ggctgcgcct ctgacagtta
     3721 tggaggcctt ctccccatat actgagaccc cgctgatacc ctttccaaag ctcctctcat
     3781 cactccttga tgccttttgc atcacctcct gtgctgtgag gtcacaggtt cccaggagaa
     3841 caagactcat tgctatgtaa tggagaattt tcatgaatgg atcagttgta tgagttcctg
     3901 cagggtgccc gacttaagat tctcgatccg tattaccgac tgtatgattt cggaggagtc
     3961 gcctacctgg tcaataacca tttcaagctg gcggagctcc tcgttcagtt cctgatagag
     4021 ctgcatcttt tccctgaaga gctctgactg gcatatcggg gcgccggtcc tgcacagctc
     4081 cctgatgact tcaatcgggt cttcgtctgc gccggtcata gtcacggggg aaccgtactc
     4141 ttcaccaggg atgatttcac tctcaactgt aatgcctttt tctgttggca gaagttgggg
     4201 gattgcaaac aaaatcaggg cagcggccgc aacactgcct atagtcatga ggagcgtcct
     4261 ccggcgaatt ccggcacgct ttttcccggt cagcatgata tcatcactat caggaggcat
     4321 ctgcagggtc cttttcaccg tgctgcggaa atctgcgtct gacgatctgt ccggacgctc
     4381 ctcctgcaat gcagcctcaa tgttttccca taccgcggat gacgggtgat gaatgggcag
     4441 atcatggagc tggctcagga tggcatcttc cctgtcaagg agagattcta tctccttcca
     4501 gagagccgcg gaaggctggt gggagggcag ctcccttatt ctttcagata atatgtcatc
     4561 aaactctctg ttcatctgac ggttttgtca gtctgtttct taacagcttc tttgcataat
     4621 tcaactgcga ctttgaagtc ccttcattta tacccagtat ttcggctacc tcctggtgct
     4681 tatagccttc tacctcaatc aggataaaca cgctccgtgc ctgtgccggc agtgagagta
     4741 tcgccttctc gaggtgttca gcggtgaaat tgaacgagct ctctgacgcc tggtcaggaa
     4801 tctcgtcggt tattacctgc agccgggcat ttttcctcag gtagctcatc gcatttctga
     4861 ttactatagt gcgcatccat gtaaacagtg acgattcaaa cctgaaggtt gccaatgacc
     4921 tgaatatctc aatgaacgca tcctgaagaa catcattgct ggcatcatga tcgcttatca
     4981 gcctgtatat cagagtgtag aggcgagcgc tatacttttc gtacagggca cgctgcacca
     5041 gcctgttgtt tcccaggcat ccccggacaa gctccctgtc gccctgattc tgttcactct
     5101 gctgcatctt tgtcttctgt ctatatagtg cagatcaggc ctgaaaaggt tggaagagga
     5161 aaaaaatttt tatgtgacca cttgacatca tggactcaga cagcctgaca ttcaaggtgc
     5221 aggctatcta tacaaaatta ccgggattgt ttgtaattta caattcttta tcgtctgaga
     5281 cgagggggcg gatgccgtta tctaccgatg cggatcacat gactgaatga actccctgag
     5341 cagcacctgc agcttccgtg tcagatggcc cggattacca tccccgataa cctgatcact
     5401 gatccgcgtc actggcacta tctctccggt ggtgttgcag ataaacgctt cgttcatgta
     5461 ggggatcatg tcggtggcga cagcctcctc aaccacgctg acatcatttg ccctggcaac
     5521 ggagataatg tttttccggg taatccctga gaggatatca ttgttttccg gatgggtgta
     5581 aagtgcttcg ttcctcacaa aaaagatatt cgaatgagct ccttcggtca caaagccgtt
     5641 ccgcacgaag cacacctctg caaatccctg gtcatgtgcc tcctggtaag gcagtacgtt
     5701 ggctatcagg gcagtggtct tgatatcgca gcggttccac cggggatcag gggtgagcca
     5761 tatagcgaca ccccgctcag cagcaagcgt attgatgttg tggcggcggg caaaaccgta
     5821 gatggttggt ttgaccggcg gatcgggaaa ggcatggttg cggggtgcaa cgccacgtgt
     5881 tgcctggaaa tagactatcg cacaatcggc gccaagcccg tttcgctcta taagctcttc
     5941 aaagatctcc tccagctttg cttcatcttc ccataccatg tttgtctcct tcatgctgcg
     6001 cctgaacctg ccggtatggc ccgggagatc aaagaagtac ccgccatacc atctggctac
     6061 ctcgtaaaca ctgtccccga aaagaaaacc ccggtcatca gggctgatca gcgcctgatc
     6121 cttcgggaga aactgaccgt taagatatac tacttccata ttttaaaatt tacactttac
     6181 cggccgggtc atgttggcct gcgactcttt tttcccgcat acgcgggatt atgaaaacca
     6241 atcgtcaaag atacattttc aatggattgg tcatacaaat ttcaacccct ccttactctt
     6301 ttattatgta cctttgcata ttcacaaaat tcgtcaatca ggatttatga gaggcaagag
     6361 gcgactaccg tcgtggctca ggatggaaag ggcaagcggg gagagttaca ccatggtgaa
     6421 gaacctcgtt gagagtcacc gcctgcatac catctgcaca tcgggcaact gcccgaacat
     6481 cggggagtgc tggaatgccg gcactgccac cctgatgatc ctcggtgaca tttgcacaag
     6541 atcatgcaaa ttctgcggca cgaagagcgg caggccgctg ccgcctgacc cgggagagcc
     6601 tgacagggta gctcaggcgg tgaagaccat gcatctgaag cactgcgtca tcacttccgt
     6661 tgaccgtgac gacctgcctg acggcggtgc tgctcactgg gcagaaacga tcaggaggat
     6721 cagggaggtg aatccgggcg tgaccatcga gacactgatc cccgacttca ggggcaaaac
     6781 agagcatatt gacatggtga ttgatgcagg gcccgatgtc atctctcata acatggaaac
     6841 agtcaggcgc ctgactcccc tgatcaggtc agttgcaaag tacgaagtaa gcctggcggt
     6901 gctcagacac atcgcttcgc gcggtgtgcg cgcaaaatcc ggtattatgc ttggcctcgg
     6961 agagagcgaa ggggaggtga ctgaaacgct ggccgatatt tatgcaaccg gttgcagaat
     7021 tgtgactatc gggcagtatc ttgctccaag ccttgagcat ataccggttg ttgagtatgt
     7081 tacgcctgag aagtttgagg agtacaggaa aacagggctt gagaccggat ttgatttcgt
     7141 tgagagcagt ccgctagtca gaagttcctt ccatgctgaa aagcatgtga aagagcgact
     7201 gcacgattta tagtgaccag ggaagttttt gtaacagatt atgataaaca acgccggcat
     7261 aaaggtaatt tttgatgatc tcggtctgag agacttcaag gagacctggg attatcagga
     7321 gcatttcttc aatctcaagg tggcggagaa gggcagaccg gagagtgatg gcaaaactac
     7381 tcctgaccgg ctgatctttg tggagcataa ccatgtttac actctgggga agagcggatc
     7441 agagcagaac ctgctgcttg actatatcca gttgcgggcc cgcgatgcct cgttctacaa
     7501 gatcgacagg ggaggggata ttacctatca tggccccggc cagctcgtgg gttatcccat
     7561 ctttgacctt gaaaagctga agcttgggct tcgcgaatat attcatctgc ttgaagaagc
     7621 aattatcagg tgtatctccc gtttcggtat tgaggggagt cgcctggagg gcgccacggg
     7681 ggtatggatt gaccccgaca agggagcccg cgcgagaaaa atttgtgcca taggcgtaag
     7741 ggcctcgcgg ttcgtgacaa tgcacggctt tgccctgaac gtgaacactg acctctccta
     7801 ctttaaccat atcaaccctt gcggcttcac cgacaaaggt gttaccagca tcgggaaaga
     7861 gacgggagaa aagagagaaa tggatgatgt gaagcaagtg atgtacaaca caatgaggga
     7921 ggtcttcgga tatgaatatt attaaaaata aatataatgg acaaaaggtg gctcttgaaa
     7981 gagaggggcg acgaacatgt cgtcacgacc ctggcgacag agatgggggt gcctgtcact
     8041 gtcgccggcc tgatggctca gcggggcatc acaacgaaac aacaggctga ggaattcttc
     8101 aacccgaccc ttgaatccct gcatgacccc ttcctgatga aggacatgaa cagggcggtg
     8161 gacaggatta ccacggcaat atcgcgaaac gaaaagatac ttgtttacgg cgactatgat
     8221 gttgacggta ccacgtcggt ggcaatgctc tattccttcc tgagggagag gcattcctgc
     8281 cttgattact acatccctga ccgttaccat gaggggtacg gactctcctc aaggggtatc
     8341 gactatgctg ccgggggggg ctgcaaactg attattgcgc ttgattgtgg aattaaggct
     8401 gttgagaagg taaaatatgc acggtcaaag ggtatagata tcattatttg tgaccatcac
     8461 ctcccgggcg agggaatccc ggatgccact gccgtccttg accccaagca gccggggtgc
     8521 ggttatccct acaaggagat gtcagggtgc ggtgtgggat tcaagctgat gcaggctatc
     8581 tgcatgttgc agggaatacc gtttgaagag attattccct atcttgacct ggttgcagtc
     8641 agcatcggct ctgacattgt gccgatgacc ggcgagaaca ggatactggc ttattacggg
     8701 ctgaagcaac tgaacgagtc accccgcctg ggatttcaga agatcattga aaaggcaaag
     8761 ataaaaaagc cgctggtcat tgaagatgtg gttttccgca tcggcccccg gatcaatgcc
     8821 gccggccgga tggagtcggg gagcagggcg gtagatctgc tggtatcgca gaattcgcag
     8881 gaagcaatga ctatctgcga tgccattgac gtcaataatg accaccgcag gaaaaaagac
     8941 agcgagatca ctgccgaagc gctccgcatg gtgagcgatg acatcaggtc aggcaacgcc
     9001 cgcactacgg tactgtttca ccggggatgg cacaaggggg tgattggtat tgtggcatcg
     9061 cggctgattg agacatatta ccggcctact gtgatactga cggagtcgaa ggatggatat
     9121 gccaccgggt cggcacgcag cgttcccgga tatgatcttt accaggcaat cgaatactgc
     9181 tctgacctgc tcgagagttt tggagggcac atgtttgcag ccggactgac cctgaagcag
     9241 gagaacctgc aggagtttcg cgagaggttt gagcaatatg tcagcgagac gataagggat
     9301 gagcacctgg tgccatcagt caatattgat gaagagattc ctcttgaaga ggtcaccatg
     9361 gaattttgcc ggcatcttga gaagtttcag ccgttcggcc ctgagaatac ggcgccggtg
     9421 tttgtctcaa gaagggtcaa gacgctctca tgccagtctg tgggtgccaa cggtattcat
     9481 ctcaagctct ccttcgccag ggagaacggt gagcctgtgg gagccattgc ctttggccag
     9541 gctgacatga ttgagacctt ccgtgagtcc ggcggtgttg ttgacatagc ctactcagtg
     9601 gagatgaatg agtttcgcgg aaagaggtca gtgcagctca atatcaagga tatcaagggg
     9661 tagaggtgtc agccatccgg cattgacctg ccggttttct gacaattgcc tgttgctaat
     9721 cgtctgttga cttttttctc acttttcctt gacattctcc tttccctgtc ctaaatttga
     9781 tggagtaatt atgtctcatt atgatgctgc ggtggaatgc agtgttaatg ctgctgtttt
     9841 ttcattttac cgtcatacat gccggtgaac cattgtttgt gcctcatggc gcgggtgcga
     9901 tgggggtggc tttctcggtt acggcaacgc cgggtcactg gaactgcttc cataaccagg
     9961 cgctgctcac tgccgtccct tcatcagggt tctcaatcgg actggagaac cggttcatga
    10021 tgccggcgct ctcatcaaag gcagtcagcg gtgtgctggc cttcaaaccc gctcccctgg
    10081 ggatcatggt ttctcattac ggtaacgctg actacttccg gattttcacg gggcttggca
    10141 gcgctgtcag aatcagtgac ggtatctccc ttggtctcca ggtagattat atctctgaaa
    10201 acggagcggg agaataccgg gatgtgtcat ttgttacatt cgagacagga gtcacatgtc
    10261 tcgttgcacc tgcgctgact ctcggggtgc atctcttcaa ccccttctct cccctgaata
    10321 cccttccttc atcaattgat gccggctttc actggaagca gtccgacgaa ctttttctca
    10381 ccctgaccgg ctcgaaggtg agcaacgagc cgctctccgt gcagtgcggc atttcatggg
    10441 atgtaatgaa aaggctgact ttgcgtaccg ggtatatgag ctcaccttct gccttcgctt
    10501 tcggtatcgg gttcaagacc gcccttctgg aggctgatgc cggatttctg cttaatggtg
    10561 ttaccggtgt tacatcttct gtgtcaatta catggttgat cagatagagt gttgaccctc
    10621 atggagacca taagcatgac catattggaa ataagattaa cccgggtatc gcgatcatga
    10681 ctttaattat tcacttatga gtttcaggag cgctgtttca gtaatgatat tgttactcct
    10741 ttcggggact ctcttctgcc aggaggaaaa cctgtcactg agggaatcag ttgaagagat
    10801 tcttgcgctg tctgatgatg ccgatccggc tgccttgctt gaggaacttg aggagctgaa
    10861 ggcttttccc gtgaatatta acggcggaga tgaaaaggag atctcacggc tctttttcct
    10921 gacagaattc caggtcatgg tacttgccga tcatgtcagg aagaacggca gcgtggtgac
    10981 cctctatgag cttgccctgc ttccggcttt tgaccgcaac acggtgctgc tgatggctcc
    11041 gtatatatcg cttaagcctg cgaagccgaa aggagggagg agctacagcc ggactctggt
    11101 gacgctgacg gcgtcagcac ggttcagcaa cgacgaaaac gctgcggagg gcatgcggtc
    11161 attgatgaaa atgaaacaca agcagggaag tttcactttc ggtctgacgg cggagaatga
    11221 ccccggcgag ccattcatct tcaaaaatgc tccgggggcc gactttctct cgggttatgt
    11281 tcagtatgaa ggcaaaggat taataaacag gattattgca ggcgactttt ccgtcagggc
    11341 gggagagggg gtgctcctga acagcagccg gtggatgggt tcatggctca gctcgccgtc
    11401 gttcatgtcg ggacgaagtg tttcatcgcc ctatacatca acagaagaga acagtttttt
    11461 caggggaata agcctcaccc tcggaggagt gaattccggc gccctgctgt ttgcctcttc
    11521 aaataaaatc gatgcccgtt tgctctttac agatgacagt gttgccaccg gggtgagaaa
    11581 ccttgtcagg ggaggcattc atgtcactga atcacagctt gcagcacgca acagcctcac
    11641 tgaaacggtg accggcctgc gtctgattgc cggtacggga aaggccaggg gcggggtgac
    11701 tgcaactgcc acctggttct ccctgccgtt tgtccccgac gtgactgagc cggagaacct
    11761 tcatgccttt gcaggcgaaa ggctgctcaa tctgtctgcc gacatcaggg cagggacagg
    11821 ctccatactg ttttttaccg aggcaggcct gggtctttcc ggtcctctga caggcactgc
    11881 aggaacagat aatccgggtc cgcaggcaga cacaaccggc acagaccctc cgggttcagt
    11941 gtcagccata gacagactga atcttcgcaa ttcatgggca gcaacagccg ggttgagggc
    12001 aaaaccatcc ggaagggtta cgtttaatct cctcgcgcgt cacttcgccc cgggatacca
    12061 cgcgttccat tccggggcct tcagggcggg atcgggatca ggcaatgaga ccggtctttc
    12121 agcctcggta caccttgaag ccgcaaggca tctgtttatc actgcagcag ctgatcacta
    12181 ccgcataccc tggccgcgat accgttcatc atcaccttcg tatggcagca gaattgagat
    12241 caagggagaa tatgttcccc gcgaaaacat ctctctgagg ttgtcatgga catcctcagc
    12301 acgggagtat gatgctgccg cggagacggg cacagccgtc ccggtgacgc gtaccaggca
    12361 gcagctgggt cttgtgtttg atatgagcat agcctcgact ttgcgtctca ccacccgggc
    12421 ttcggcatcc tttatccatc cttcgcagga gagaggttat ctgctcggcc aggacttttc
    12481 ctggtcattc cggcgtgtac ctttgaagct ctggttcagg tatgcacttt gctcaacggg
    12541 aggctgggac agccgtctct atgcctggga gaatgacctt ctttcctctt tcaccattcc
    12601 ggcactttac ggagagatgg cgcgctcatt cctgatgctt tcctggaagc ccgctgaatt
    12661 cattgaggcg agggtgaagt acggttatac agatggcagg aataactcat cgggcggtat
    12721 gaaacaagaa ttgaagggtc agatcaagat tgagttttaa gtgacggcca acaggccatg
    12781 cccggatcat ttctgaagtc agtttagcat taatttataa agaagccttc tgtacatcac
    12841 tggaggatac agaaggaacg attttagcac acaaccctac tgaaaaggtg attgtaacga
    12901 tttcaaattt caaaacagag gttgacttta gccttgatga gaatatgaga agggttataa
    12961 cctcggaaat gggtcaacat aaaatagata gtattaaaac gcgtgaagat ttaatatgct
    13021 actataagaa tctcacccct ctttttcagc ccggatattc cagccttagc gatgacaatc
    13081 attatatttt tgcaaaagta gagtacttat tggcgcaaga gtgttttcag gacaactgta
    13141 aatcactgac aagaagggag attttagaag
//
LOCUS       DAOPBK010000063        13117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4724_length_13117_cov_5.043408, whole genome shotgun sequence.
ACCESSION   DAOPBK010000063 DAOPBK010000000
VERSION     DAOPBK010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_13117_cov_5.043408"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /gene="thyA"
                     /locus_tag="PLA17_05205"
     CDS             <1..371
                     /gene="thyA"
                     /locus_tag="PLA17_05205"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPK84845.1"
                     /translation="LPPCHILFQFYVAGDRLSCQLYQRSADIFLGVPFNIASYSLLTM
                     MMAQVTGYKPGEFIHTLGDAHIYLNHIDQVKLQLTRDPRRLPVMTLNPDVMDILGFTY
                     ADFSLSEYDPHPAIKGEISV"
     gene            373..861
                     /locus_tag="PLA17_05210"
     CDS             373..861
                     /locus_tag="PLA17_05210"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPK84846.1"
                     /translation="MITIIVAIDRNNGIGYNNRLLAHIPGDLRRFREITMGHCLIMGK
                     NTWESLPNRPLAGRKNIVLTDDERDYFSGAERALSIDEALGLCDPGREIFIMGGGSVY
                     RQFLPKADRLMVTHIHREYQADTFFPKIDPKEWYVSEQEDFFSEDPSELSFSYTTYLR
                     RR"
     gene            complement(996..2210)
                     /gene="clpX"
                     /locus_tag="PLA17_05215"
     CDS             complement(996..2210)
                     /gene="clpX"
                     /locus_tag="PLA17_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPK84847.1"
                     /translation="MDKCSFCGRTKKEANLLIAGLEGHICDHCIEQAHNIMTEELGGR
                     SSFNLDSIKLRKPDEIKNFLDQYVIGQDRAKKILSVAVYNHYKRLMQKTDSDDVEIEK
                     SNIILVGKTGTGKTLLARTIARMLHVPFTIVDATVLTEAGYVGEDIESLLTRLLQNAD
                     YDVKAAEKGIVFIDELDKIARKGDNPSITRDVSGEGVQQGLLKLLEGAVVNVPPQGGR
                     KHPEQKMIPVDTRNILFICGGAFEGIEQKIAHRLNTTVVGYTASRSRDHYDKDDLLKY
                     IAPQDLRAYGLIPEIIGRLPVLTHLSPLDPRALRAILTEPKNAITKQYVKLFAMDGIK
                     LAFTPEVLDYIVDKAVEFKLGARGLRAICEVIMMDAMFELPSGDVKEFTVTTEYASNK
                     LERVDMKVLKAS"
     gene            complement(2222..2899)
                     /gene="clpP"
                     /locus_tag="PLA17_05220"
     CDS             complement(2222..2899)
                     /gene="clpP"
                     /locus_tag="PLA17_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPK84848.1"
                     /translation="MPDINDFKKYAADRSGISSMTMHRFISIANSYISPTIIEERQLN
                     VASMDVFSRLMMDRIIFLGLPIDDYVANIIQAQLLYLDSVDPDKDIQIYLNTPGGAVH
                     AGLGIYDTMQYISADVATICTGMAASMGAVLLSAGAKGKRSALKHSRIMIHQPMGGAE
                     GQASDIEIAVREILKAKRELYEIIALHTGKTVEKVEKDSDRDYWMTAAEAKEYGMIDE
                     VLIRNKN"
     gene            complement(2953..4284)
                     /gene="tig"
                     /locus_tag="PLA17_05225"
     CDS             complement(2953..4284)
                     /gene="tig"
                     /locus_tag="PLA17_05225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPK84849.1"
                     /translation="MNVTRENIDALNATVRIDIDKADYEEKVEKKLREYRRTASIKGF
                     RPGHVPVQMIRKMYGTTVLIDEVNNIVSETLSEYIKNENLDILGDPIPKNDGHIFDPE
                     KSDQFSFTFELGLAPEFEVNLNKKHKLTRYLIEPDTKMIADSIDSYTRRYGEFITAEA
                     AEEKDMLKGIIESSDGSVKNDAATLSVEMVKDEEIKNSFLGKQAGDTVEFDLRRAFPN
                     DYEIAGLLKKQKDEVKEFQGQYSIQIMEISRFVPAENNQELWDKVYGEGTVKSVTEFE
                     AKVTEEIREFFNRETEYKLRTDARDLALAKTAFELPEEFLKRWLLRVNEKTTAEDIEK
                     DFDHFREDIRWQLIKNRVAKDNEVKITDEEILGEAKAFTRAQFAQYGLQYVSEEQVTS
                     FAINFLKNEEEARRIAEKVLDTRVLGLLIDAVRVDEKRVTPEEFNKLFGNK"
     gene            complement(4363..4447)
                     /locus_tag="PLA17_05230"
     tRNA            complement(4363..4447)
                     /locus_tag="PLA17_05230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4411..4413),aa:Leu,seq:tag)
     gene            complement(4504..5229)
                     /gene="lptB"
                     /locus_tag="PLA17_05235"
     CDS             complement(4504..5229)
                     /gene="lptB"
                     /locus_tag="PLA17_05235"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPK84850.1"
                     /translation="MRLYTTNLVKRYKTRTVVNEVSVDVEQGEIVGLLGPNGAGKTTT
                     FYMIVGLIRPNGGQIWLDNTEITDYPMYRRARLGIGYLAQEASVFRKLSVEDNLMAVM
                     ELSDMTRQERKERQESLLEEFGLTRIRKSLGIQLSGGERRRTEIARALALKPNFILLD
                     EPFAGVDPIAVEDIQMIVSNLKDKNIGILITDHNVHETLSITDRAYLLFEGKILKQGT
                     AAQLADDEQVRKVYLGKNFELRR"
     gene            complement(5240..6340)
                     /gene="tatC"
                     /locus_tag="PLA17_05240"
     CDS             complement(5240..6340)
                     /gene="tatC"
                     /locus_tag="PLA17_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPK84851.1"
                     /translation="MAPIMAGKSKAKNSNPDGEMTFLQHLEELRWHIIRSLVAIVIGA
                     IVAFMFKDIIFDHIILAPNNPDFITNRLLCRLADMVNVPLLCINQNPVELISIKLTGQ
                     FTTHITISLVAGLILAFPYVFREFWSFFRPALYEKERKYARGAVTMASLLFLAGIIFG
                     YFIIAPLSINFLGTYRVSDLVTNQINITSYIGSVTSVALASGITFELPIVVFFLARIG
                     VLTPEFMRKYRRHAIVVVLVVAAVITPPDVFSLILVSIPLLILYEVSIFLAARVVRQR
                     EAAEAKEAAEEAKAEAARRKKAAEEAADAKDAEAAADKQKAAEAAEKEKEAETKATAE
                     AVAEKNRTNPENPDSQGNNDSRPGEPDETAGN"
     gene            6569..8746
                     /gene="recQ"
                     /locus_tag="PLA17_05245"
     CDS             6569..8746
                     /gene="recQ"
                     /locus_tag="PLA17_05245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HPK84852.1"
                     /translation="MHTDASIHDYLKRYFGFDMFKGNQGEIIKSVLAGNDTFVLMPTG
                     GGKSLCYQLPAVMMDGTAVVISPLIALMKNQVDAMRNFNTTDDVAHFLNSSLTKTAIA
                     KVKQDVLQGNTKLLYVAPESLTKEENIEFLKSVKVSFYAVDEAHCISEWGHDFRPEYR
                     RIRPIIDTISRAPIIALTATATLKVQHDILKNLGITDAMVFKSSFNRPNLYYEVKAKQ
                     DATREIIKYIKNNPGKSGIIYCLSRKKVEELAETLKVNDIKALAYHAGMDSQTRTSNQ
                     DKFLMEEVDVIVATIAFGMGIDKPDVRYVIHFDMPKSLEGYYQETGRAGRDGGEGRCI
                     AYYSYDDIVKLEKFMQGKPIAEQEIGKQLLLETVSYAESSTCRRRILLHYFGEEYTQD
                     NCEACDNCLHPKTQFEGQDYVVSMLETVLAVKEKHKADHIAKILAGTMNSAIKSYKHH
                     KLETFGIDEEKDERFWNMVIRQALINKLLDKDIENYGLLGLTPKGHEFLDKPQSFLLQ
                     EDHDYTETEDEEASFGARTAAADEELLSILKDLRKKIAKQAGQPPYNIFQDPSLEEMA
                     IQYPVTLEELQNITGIGAGKAQRYGREVVEVIKNYVADKEIIRPQDMVVKSVVNKSGL
                     KVYIIQSIDRKMPFEDIADAKGIEYDELLTEIEAIVGGGTKLNIDYYIDTVIDEDRQE
                     DIYSYFMEEAESGSLEEAINELAGEFEEEEIRLVRIKVLAEGN"
     gene            complement(8942..10186)
                     /locus_tag="PLA17_05250"
     CDS             complement(8942..10186)
                     /locus_tag="PLA17_05250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK84853.1"
                     /translation="MWWLVIPVIIYSSGLLALWASIARRSNGADAGDAGQPEAGEAMA
                     ASMTGQHPLTGDNGGVPETDALHGAGVSEAPQEPTGLPAVSVIVAARNEERHIETLLG
                     SLVKQDYPDGLLEIIIVNDNSTDRTPVAASEFIEADKGMSAHRIRLIYNPFSGKKRAV
                     RYGIEKSSGEVILTTDADCVVGPEWVRRHASWYTSGGPDVVLAPVIQKPAEGFWHGFG
                     AYEFAALQAITEASTVPGHPVMCNAANMSFRRDVYLRHAEELHPDLPSGDDMFLLHAV
                     KRDGGTVRHEGSNAAAAVTAGAVTAAALLRQRARWASKAGRYRDPATLTLAAATAACN
                     AAVATAVVTACMSVEYLLPAAVMYGVKALPDYLLIATELKKRGARVKAVLFMVSEILY
                     PFWFVTVAVMSLFPSSRRFRRR"
     gene            complement(10199..10405)
                     /locus_tag="PLA17_05255"
     CDS             complement(10199..10405)
                     /locus_tag="PLA17_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84854.1"
                     /translation="MITIRKATPDDAPAIISFHQAMAMETEGMECGTAGLRLYVEADN
                     VLAQQVYEAMGIDGNHYRTFKWMV"
     gene            10981..11442
                     /locus_tag="PLA17_05260"
     CDS             10981..11442
                     /locus_tag="PLA17_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HPK84855.1"
                     /translation="MNKSLRLALIEKLVREHVIGSQDELLRLMEGTGMKCTQATLSRD
                     LRQLGISRGFDGSGGYRYILPSGRDESAEMVPDNTAAAISGMVWARGVLLISTPPGFA
                     SAVAIRIDLAGRYEIAGTIAGDDTILLIPRDNISNEAVEECLRAIFHSDIK"
     gene            11454..12707
                     /locus_tag="PLA17_05265"
     CDS             11454..12707
                     /locus_tag="PLA17_05265"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPK84856.1"
                     /translation="MKKVMLAYSGGLDTSVILKWLINKGYEVIAYVADVGQDDDFGAV
                     REKALAIGASSVIIDDLKREFVTDYIFQAVKANAVYEGRYLMGTALARPLIAKKQIEA
                     AIEQEADYVAHGSTGKGNDQVRFELAYYALKPDIRVIAPWKDAEFLSQFHGRSDMIRY
                     AAVHSIPVKASISKPYSEDDNLMHISHEAGILEDPLYFAGKDILQKMVMPMDAPDKET
                     HISITFKDGIPVNVTNKDDGTSYSDPLELFTCLNRLGGANGIGLLDMVENRFVGIKSR
                     GIYETPGATILYAAHRDIEGIAMDREVMRLRNMLAPVFAELVYNGFWFSPEMEFLKAA
                     IDKSQEVIDGVVHMILYKGNVIIEGRESPSSLYNKELSSMDIEGGFNQSDSLGFIRIN
                     AIRLMAHNAIMEANKKKVTEDAPVG"
     gene            12691..>13117
                     /locus_tag="PLA17_05270"
     CDS             12691..>13117
                     /locus_tag="PLA17_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84857.1"
                     /translation="MHLWDKGGTARESVISFTCDSDRLYDARLAPYDIVGSMAHAVML
                     ERCGLITAGEKEALLSGLARLLDEVLQSDFSIGKEFEDIHSWVEIRLGDMAGEGAGVN
                     RDRQGTRDAHSAAGELHAGSPGAQDARSAAGELHAVSPGA"
BASE COUNT         3086 a         3289 c         3372 g         3370 t
ORIGIN      
        1 cccttccgcc ctgccatatc ctgttccagt tctatgtggc cggcgacagg ctctcatgcc
       61 agctctatca gcgcagcgct gacatcttcc tgggcgtacc tttcaacata gcttcctatt
      121 cgttgcttac catgatgatg gcgcaggtga ccggttacaa gccgggcgag tttattcaca
      181 ccctgggcga tgcacatata tacctcaatc acattgacca ggtaaaactg cagctcaccc
      241 gtgatccgcg caggcttccg gttatgaccc tgaatcctga tgtcatggac atcctcggtt
      301 tcacctacgc tgacttctct ctttcggaat atgacccaca cccagccata aaaggcgaaa
      361 tttcagtata aaatgatcac aataatagta gcaattgacc gcaacaacgg catcggatac
      421 aacaacaggc tgttggcaca catcccgggc gacctccgga gattcaggga aattaccatg
      481 ggccattgcc ttatcatggg caagaatacg tgggagtcac tcccgaacag accgcttgca
      541 ggcaggaaga atatagtact taccgacgac gaacgtgact acttcagtgg tgcagaaaga
      601 gccctttcaa ttgatgaagc gctgggtctc tgtgatccgg gcagggaaat attcatcatg
      661 ggaggcggat ctgtatacag gcagttcctg ccgaaggcag acaggctgat ggtgactcat
      721 atccacaggg agtatcaggc tgacaccttc ttcccgaaga ttgatccgaa ggaatggtat
      781 gtttcggaac aggaggattt cttttcggaa gacccctctg aactcagttt ttcatatacc
      841 acctatctca ggcgcaggtg actttacggc ctggtctcat actgcactat gggcaccggg
      901 atcatcccgg gcgcaggtga ctttcaggtt tggcggtata cagtgctatg ggcaccgggg
      961 tcatcccagg cacagataag gtgactttcc gcgaattatg atgccttgag cactttcatg
     1021 tccaccctct cgagcttatt ggaggcatat tcggtggtga ctgtaaactc cttcacatcg
     1081 cctgacggca gttcgaacat cgcgtccatc atgatgactt cgcagatggc acgaaggcct
     1141 cttgcaccca gcttgaactc cacggccttg tctacaatgt agtcaaggac ctcaggggta
     1201 aaggctagtt tgataccgtc catggcaaac agtttgacat actgttttgt gatggcgttc
     1261 ttcggctctg tcagaatggc tctcagcgcc ctggggtcaa ggggagagag gtgggtcagc
     1321 accggcagac ggccgatgat ctcagggatg agaccgtaag cgcgcaggtc ctggggtgca
     1381 atgtacttca gcaggtcatc cttatcatag tggtcacgtg agcggctggc agtgtatccc
     1441 accacggtgg tgttgagcct gtgtgctatc ttctgctcta tcccctcgaa tgcacctccg
     1501 cagatgaaga ggatattcct ggtatcaaca ggtatcatct tctgctcggg gtgtttcctg
     1561 ccgccctgcg ggggcacgtt cacaactgct ccttcgagga gcttgagcag accctgctgg
     1621 acaccctcac ctgacacgtc acgggtgatt gagggattat cgcccttgcg ggctatcttg
     1681 tcaagttcat caatgaacac tattcccttc tctgcagcct tgacatcata gtcggcattc
     1741 tgaagcagac gggtaagcag gctctcgata tcttcaccca cataacctgc ctctgtcagg
     1801 acggttgcat caactattgt aaagggtaca tgaagcatac gggctattgt gcgggccagc
     1861 agcgtcttgc cggtacctgt tttacccacc aggatgatgt tcgatttctc aatctccaca
     1921 tcatcactgt cagtcttctg catcagcctt ttgtagtggt tatagacggc taccgagaga
     1981 atcttctttg ccctgtcctg cccgattaca tattgatcaa ggaaattctt tatctcatcc
     2041 ggcttgcgga gcttgatgct gtcaaggtta aagcttgacc tgccacccag ctcctctgtc
     2101 attatattat gtgcctgctc tatgcaatga tcgcatatat gaccctcgag accagcaatg
     2161 agaaggttag cctctttctt ggtacgtccg cagaacgagc atttatccat caggggtata
     2221 attagttttt gttcctgatc agaacctcgt caatcatacc gtattcctta gcctctgcgg
     2281 ctgtcatcca gtagtcgcgg tctgagtctt tttcaacctt ttcgacagtt ttgccggtat
     2341 gtaatgcgat tatctcgtaa agttccctct tggccttcag tatctccctg acagctatct
     2401 cgatatctga tgcctgtccc tctgcgccgc ccatcggctg atggatcata atacgggagt
     2461 gtttgagtgc cgagcgtttg ccctttgcgc ctgccgaaag cagcactgct cccattgagg
     2521 cagccatgcc ggtgcagatg gtggccacgt cggcagagat gtactgcatt gtgtcataga
     2581 tgcccagccc tgcatgtact gcaccgcccg gggtgttgag gtagatctgt atgtccttat
     2641 caggatcaac cgagtcaaga tagagcagct gagcctgtat gatattggcc acatagtcgt
     2701 caataggaag gccgaggaag ataatacggt ccatcataag acgcgagaag acatccatag
     2761 atgccacgtt cagctgccgt tcctcaataa ttgtcgggga gatatagctg ttggcgatag
     2821 agatgaaacg gtgcatcgtc atgctgctga tgcctgaccg gtcagcggcg tattttttaa
     2881 aatcattgat gtctggcatg gtctgaatct ttgattgaat gccaaatata cgaattaata
     2941 ggctttaagg tttcacttat tgccgaatag cttgttgaat tcctcgggtg ttaccctctt
     3001 ctcatccacc ctgacggcgt caatcagaag gccgagcacc cttgtgtcaa gcactttttc
     3061 ggcgatgcgg cgggcctcct cctcattttt caggaaatta atggcaaatg atgtgacctg
     3121 ctcttcagaa acgtattgca ggccatactg ggcgaactgg gcccgggtaa aagccttcgc
     3181 ctccccaaga atctcctcgt cggtgatctt cacctcattg tcctttgcaa ccctgttctt
     3241 tatcagttgc cagcggatgt cttcacggaa gtgatcgaaa tccttctcta tatcctctgc
     3301 ggttgttttt tcattgaccc tgagcaacca tctcttgagg aattcctcag gcagttcaaa
     3361 ggcggttttt gccagagcca gatcgcgggc atcagtgcga agcttgtatt cagtctcacg
     3421 gttgaagaac tccctgatct cttcagttac ctttgcctcg aactcagtga ctgatttcac
     3481 ggttccctct ccgtagacct tgtcccatag ctcctggttg ttttcagccg gaacgaagcg
     3541 ggatatttcc attatctgta tcgagtattg cccctggaac tctttaactt cgtccttctg
     3601 cttctttagc aggcctgcaa tctcataatc attcgggaat gccctgcgaa gatcgaactc
     3661 aacggtatct ccggcttgtt tgccaaggaa agagttcttt atctcctcat cctttaccat
     3721 ctccaccgac agggtggcag catcattttt caccgatccg tcagaagact ctatgatacc
     3781 cttgagcatg tccttctcct ctgctgcttc ggcagtgatg aactcgccgt acctgcgggt
     3841 gtagctgtca atggagtcgg caatcatctt tgtgtcaggt tcaatgaggt agcgggtgag
     3901 cttatgcttc ttgttaaggt tcacctcaaa ttcgggtgcc agccccagtt cgaaggtgaa
     3961 gctgaattga tccgatttct caggatcaaa tatatgtccg tcatttttcg gaatcgggtc
     4021 acccaggatg tcaagatttt catttttgat gtattcagaa agagtttccg aaacaatgtt
     4081 gttgacctcg tcgatcagga cggtggttcc gtacatcttt ctgatcatct ggacaggcac
     4141 gtgtccgggc cggaacccct ttatgctggc tgttctccgg tattccctga gtttcttttc
     4201 aaccttctct tcatagtcgg ctttgtcaat atcaatcctg actgttgcat tcagtgcatc
     4261 aatgttctct ctggtgacat tcatcttgta aaagtttact attgatttaa aaaccgccat
     4321 aacctgcgcc atgcggcgaa gtgtcagagc ggttcaggtg tctggtgcgg atgaagggac
     4381 tcgaaccccc acgccctgag gcacaagatc ctaagtcttg ctcggctacc aattacgaca
     4441 catccgcaaa aagtggtgca aaattagtaa aaattacgaa gttgcaaaag atgcgaaatt
     4501 aaattacctc ctgagttcga agttcttacc gaggtagact tttcttacct gttcatcatc
     4561 agctagctgt gcagcagtgc cctgtttcag tatcttcccc tcgaacagga ggtaggccct
     4621 gtcagtgata gagagcgttt cgtggacgtt atggtcggtt atcaggattc ctatattttt
     4681 atctttcagg ttagagacga tcatctggat atcttcgaca gcaatggggt caacgccggc
     4741 gaagggttca tcgagaagga tgaagttggg tttgagtgcc agggcgcggg cgatttccgt
     4801 acggcggcgt tcgcctcccg acagttgaat gccgagactt ttgcgtatgc gtgtcaggcc
     4861 gaactcttcg aggagtgact cctgccgctc cttcctctcc tgtctggtca tgtcagagag
     4921 ttccatgacg gccatgaggt tgtcttcaac gctcagtttg cggaagacgg aggcttcctg
     4981 tgccaggtag ccgataccga ggcgtgccct gcgatacatg ggatagtcgg ttatctcggt
     5041 gttgtcaagc catatctggc ctccgttcgg ccgtatcagt cccacaatca tgtagaatgt
     5101 ggtggttttg ccggcgccgt tgggaccgag gagacctact atttctccct gctcaacatc
     5161 gacggagacc tcgttgacca ctgtgcgtgt cttgtaccgt ttgacaagat ttgttgtgta
     5221 aagcctcata gtttcatggt tagttgcctg cagtttcatc cggttctccc ggccggctgt
     5281 cattattgcc ctgactatca gggttctccg gattagtccg attcttttcg gcaactgctt
     5341 ctgctgtcgc ctttgtttct gcttcttttt ccttttcggc tgcctcagcc gctttttgct
     5401 tatcggctgc tgcttctgca tccttcgcgt cggcggcctc ttctgctgcc tttttccttc
     5461 tggccgcctc tgccttcgcc tcttctgccg cctctttcgc ctcggccgcc tccctctgcc
     5521 tgacgaccct tgctgccaga aagatactaa cttcatagag gataagcagc ggaattgaca
     5581 caagaatgag gctgaatacg tcaggaggcg taatcactgc tgcaaccacc agtaccacta
     5641 caattgcatg ccggcggtat ttcctcatga attcaggtgt cagcaccccg atgcgtgcca
     5701 ggaagaagac aacgataggg agttcgaagg ttatcccgga ggcgagagcc acagatgtga
     5761 ctgagccgat ataggatgtg atgttgatct ggttggttac caggtcactg acacggtatg
     5821 ttccgagaaa gtttattgag agcggggcta tgataaagta accgaagatg ataccggcga
     5881 ggaacagaag tgaagccatg gttacggccc cgcgggcata ttttcgttct ttctcgtaaa
     5941 gggccgggcg gaagaacgac cagaattccc ggaagacata agggaatgcc agtatcaatc
     6001 ctgccaccag tgatatagtt atatgtgtgg tgaactgacc tgtcagcttg atgctgatca
     6061 gttccacggg gttctggttg atgcagagca acgggacgtt caccatgtcg gccagccggc
     6121 agagaagcct gtttgtaata aaatccggat tgttcggggc aagaatgata tgatcaaaga
     6181 ttatatcctt gaacataaat gccacaattg ctccgatcac gatggccacc agtgacctta
     6241 tgatgtgcca tctcagctcc tcaaggtgct ggaggaaggt catctcacca tccgggttgc
     6301 tgttcttcgc tttgctttta ccggccataa tcggcgccat tgttgaagcg tgtaaagata
     6361 gataaaactt aggtttcaaa gcaatatacc ctgcttcctg ctgcgttact tactattgga
     6421 gggatattta ttcccatagc tgacaaaaac ttttgatata tatttttatt tgagttaaat
     6481 ttgagtgaag ggtaacatgt tggataaact ttgcgtaact tgttatataa ttattaacgt
     6541 ctgattgcag gcggagagga gttgttatat gcatactgat gcttccatac acgactatct
     6601 gaagaggtat ttcgggtttg acatgttcaa gggcaaccag ggagagatta ttaaaagcgt
     6661 tcttgcaggc aatgatactt ttgtgctgat gcccaccggt ggcggcaagt cactctgtta
     6721 ccagctgcct gcagtgatga tggatggtac ggcggtggtt atctcgccac tcattgctct
     6781 gatgaagaac caggttgacg ccatgcgtaa cttcaacacc actgatgatg tggcgcattt
     6841 tcttaattct tcacttacca aaacagccat tgccaaagta aagcaggatg tactgcaggg
     6901 caatacaaaa cttctttatg ttgcgccgga gtcacttacc aaggaggaaa acatagaatt
     6961 cctcaaaagc gtgaaagtct ccttctatgc cgttgacgag gcacactgta tttcagaatg
     7021 gggacacgac ttcaggcctg agtaccgccg gatcagacct attattgata ctatttcccg
     7081 cgcacccatt attgctctta ctgcaaccgc caccctgaag gttcagcatg acatactcaa
     7141 gaacctgggt attactgatg caatggtatt caagtcctct ttcaacagac ccaacctcta
     7201 ttatgaggtg aaggcaaaac aggacgccac ccgcgagatc atcaaataca taaaaaacaa
     7261 tcccggcaag tcaggcatca tctactgcct cagccgcaag aaggtggagg aactggctga
     7321 gacattgaag gtcaatgata tcaaggccct tgcataccat gcaggcatgg actcgcagac
     7381 gcgtacctca aaccaggata agttcctgat ggaggaggtg gatgtaattg tggccactat
     7441 cgcctttggc atgggtatag acaagcccga cgtccggtac gtcattcact ttgacatgcc
     7501 caagagcctt gagggatact accaggagac cggacgtgcc ggccgtgacg ggggagaggg
     7561 gagatgcatc gcttactaca gctatgatga tatcgtcaag ctcgagaagt ttatgcaggg
     7621 caagccgatt gccgagcagg agataggcaa gcaactgcta cttgaaacag tttcctatgc
     7681 agaatcatca acctgcagac gccgtatcct gctccattat ttcggcgagg agtacacaca
     7741 ggataactgt gaggcctgtg acaactgcct gcaccccaaa acccagttcg agggacagga
     7801 ttatgttgtc agcatgcttg aaacggtgct ggcagtgaag gagaaacaca aggcagatca
     7861 tattgccaag atccttgccg ggacgatgaa ctccgccatc aaatcataca agcaccataa
     7921 actggagaca ttcggcatag acgaggagaa ggatgagcgt ttctggaaca tggtgatcag
     7981 gcaggctctc attaacaagc tgctggacaa ggatatagag aactatgggt tgctcgggct
     8041 taccccgaag gggcatgaat tccttgataa gccccagagc tttttgctcc aggaggatca
     8101 tgactacacc gagacagagg acgaggaggc atcctttgga gcgcgtactg ctgcagccga
     8161 tgaggagttg ctgagcatac tcaaggatct caggaagaag atagcgaagc aggccggtca
     8221 gcctccctat aatattttcc aggacccctc gctggaggag atggcaattc agtacccggt
     8281 taccctggag gagttgcaga acatcactgg catcggagcc ggcaaggcgc aacgttatgg
     8341 cagggaagtg gttgaagtga tcaagaacta tgtagctgac aaggagatca tcaggccaca
     8401 ggatatggtt gtgaagtcgg ttgtcaacaa gtcgggtctc aaggtttaca tcattcagag
     8461 cattgaccgc aagatgccgt tcgaggatat tgccgacgcc aagggcattg aatatgatga
     8521 gcttctgaca gagatagagg ccattgtggg tggcggcacg aaactgaaca ttgattacta
     8581 tatagatact gttattgacg aggaccgtca ggaagatatc tactcatatt tcatggaaga
     8641 ggctgagagc ggttccctgg aggaggctat caatgaactt gccggcgagt ttgaggagga
     8701 ggagatccgc ctggtacgta tcaaggtgct tgcggagggg aattagggat gccttcggtt
     8761 atcagctggc agtcagcaga tttttttgcg gttggcgtat ccggaagtgt ggcccggata
     8821 tcgggagaag tatttattgc acatggtgca ttctgcgtag tggttcgggt gccggcaaag
     8881 cagtttgttg cgcctggcgc attcagcatt atccaccctg cttgttttta ccctggtatg
     8941 tttatcgcct cctgaatctc ctcgacgacg ggaacagact catcacagcc accgtcacga
     9001 accagaaggg gtataatatc tctgacacca tgaacaacac cgctttcacc ctggcgcctc
     9061 tcttcttcaa ttcggtggca atcagcagat aatcgggcag agctttgacg ccgtacatta
     9121 cagctgccgg cagcaggtat tcaacagaca tgcaggcagt tactacggcg gtggcgacgg
     9181 cggcgttgca cgccgccgtc gctgcggcga gggtgagagt ggcagggtca cggtaacgac
     9241 cggcttttga tgcccatcgt gcgcgctggc gcagcagagc cgcagccgtg accgcaccgg
     9301 cggtcaccgc cgccgccgcg ttgctgccct catgcctgac agtgccgccg tcacgcttca
     9361 cggcatgaag caggaacatg tcatcacctg acggcaggtc gggatgcaac tcctctgcgt
     9421 gacggaggta gacatccctg cgaaatgaca tgttggctgc attgcacatt accgggtggc
     9481 ccggcaccgt ggaggcttcg gttatggcct gcagcgcagc aaactcataa gctccgaaac
     9541 cgtgccagaa accttcagcc ggcttctgta taaccggtgc gagaacaaca tccggcccac
     9601 cactggtata ccatgaagca tgccggcgca cccattccgg cccgacaaca caatcagcgt
     9661 cagtagtcag gataacctca cccgatgatt tttcaattcc atatctcact gccctcttct
     9721 tgcctgaaaa agggttgtaa ataagcctta tacgatgagc agacatgccc ttgtcagcct
     9781 caatgaattc actggctgct accggggtcc ggtcagttga attgtcattg acaataatta
     9841 tctcaagaag gccatccgga taatcctgct tcaccaggct cccgaggagg gtttctatat
     9901 gtctctcttc attgcgtgct gcaacaatca cacttaccgc gggcagtcct gttggctcct
     9961 gtggagcctc agaaacacct gctccatgaa gagcgtcagt ttcaggaacc cctccgttat
    10021 caccggttaa cggatgctgt ccggtcatgc tggcggccat agcctcacct gcttcgggct
    10081 gtcctgcatc ccctgcatct gcaccattac tgcgccgggc aatgcttgcc cacagggcca
    10141 gaagtccaga ggagtaaatt attacaggta ttacaagcca ccacatgaca gactcctgtt
    10201 acaccatcca cttgaaggtc cggtagtggt ttccgtctat tcccatagcc tcgtagacct
    10261 gctgtgctaa cacattatca gcctcaacgt agaggcgcag gccggctgtt ccacactcca
    10321 ttccctcagt ctccatggcc atggcctgat gaaaggagat gatggcgggg gcgtcatcgg
    10381 gggttgcttt gcggatagta atcattgcta tgatgtttgt ggcattatct cagtttgcaa
    10441 ttcctgatgc tctgcgcgca ccgggttttt cgtatcagtg atctgcactt ccttatccct
    10501 ttcgggaatc agccttgcgg tctcttcttc agcacttcct tatccctttc gggaatcagc
    10561 cttgcggtct cttcttcagc atttccttat ccccctcaga aatcagcctt atggtatgtt
    10621 cttcaactct tctttatccc tttcgggaat cagccttgcg gtccggaaca gtcagattta
    10681 cccgcctcac cagtattatg ggctctcgag ccaggaagca gccccctttg aatatcctcc
    10741 ttcatcattc ctgtccggat tgtaaaaata caatttttgt gactggcaac agttcacggt
    10801 attttttata taatcgcttg taactgccag attatcaaga tatatcactc ttccccaatg
    10861 agcactctga ttaattcatc atatgtctgt taacaaaaat tcaccgacct gccagaccaa
    10921 aatgcttgta attattaaaa taatgcataa atttgcacac tatatgtata aaaatacaat
    10981 atgaacaaat cattacgact ggcgcttata gagaagcttg tcagggagca tgtgatcgga
    11041 tcacaggatg agttgctcag gttgatggag ggcacaggga tgaagtgtac gcaggcgaca
    11101 ctctcccgcg atttaaggca gctgggtatc agcaggggat ttgatggcag cgggggttac
    11161 cgctatattc ttccctcagg cagggatgag tctgctgaga tggttcctga caacactgcg
    11221 gctgcaattt ccggtatggt atgggcaagg ggagtgctgc taatcagcac cccgccgggg
    11281 tttgcatcag cagtagccat caggatagac ctcgccggcc gttacgaaat tgccggtaca
    11341 attgccggtg acgacacaat cctgctgatc ccgcgtgaca atatcagcaa cgaggcagtt
    11401 gaagagtgcc tccgcgcaat tttccattca gatattaaat aacagagatc acaatgaaaa
    11461 aagtaatgct tgcatattcc gggggtctcg atacctccgt gatactcaaa tggctcataa
    11521 acaaaggata cgaagtaatt gcctatgtgg ctgacgtagg gcaggatgat gatttcgggg
    11581 ctgtccgtga gaaggcactt gcaataggag cctcctcggt gataatagat gacctgaaaa
    11641 gggaattcgt cacagattat attttccagg cagtgaaggc aaacgctgtt tacgaaggcc
    11701 gctatcttat gggcacggct ctggcacgtc cgctgattgc gaagaagcag attgaggctg
    11761 ccatagaaca ggaggctgat tatgttgccc atggatcaac cggcaagggg aatgaccagg
    11821 tgcgtttcga gctggcctac tatgccctta aacctgatat cagggttatt gcaccctgga
    11881 aggatgctga atttctttcc caattccatg gcaggtcaga catgatcagg tatgctgccg
    11941 ttcacagcat tccggtaaag gcatccatct ccaagcccta cagtgaggat gacaacctga
    12001 tgcatatcag ccatgaggca ggtatccttg aggatccgct ctacttcgcc gggaaggata
    12061 tccttcagaa gatggtgatg ccaatggacg cacctgataa ggagacacat atatccatca
    12121 ctttcaagga cggtatcccg gtgaatgtca ccaacaagga tgacggcaca agctacagcg
    12181 accccctgga gctctttacc tgtctcaaca ggcttggcgg cgccaacggg ataggcctgc
    12241 ttgacatggt tgaaaacagg ttcgtgggga tcaagtcgag gggtatatat gaaactcccg
    12301 gagcaaccat cttgtatgct gcccacaggg acattgaagg tatagccatg gaccgcgagg
    12361 tgatgcgcct gcgcaacatg cttgccccgg tgtttgctga gctggtttat aacgggttct
    12421 ggttctcgcc cgagatggag ttcctgaagg ctgccataga caagagccag gaggtaatag
    12481 atggggtggt gcatatgatc ctttacaagg ggaacgtcat catcgaggga cgtgagtcgc
    12541 cttcttcatt atataataag gagttgtcga gcatggatat cgagggaggt ttcaaccagt
    12601 ctgacagtct cggattcata cgtataaacg caattcgtct gatggctcac aatgccatca
    12661 tggaggcgaa caaaaagaaa gtgacggaag atgcacctgt gggataaggg aggcactgcg
    12721 cgggaatcgg tcatcagctt cacatgtgac agtgacaggc tgtatgatgc gaggctggcg
    12781 ccttatgaca ttgtggggtc aatggctcat gcagtcatgc ttgaacggtg cgggctgata
    12841 acggccggtg agaaggaggc tcttctgtcc ggccttgccc ggctccttga tgaggtgttg
    12901 cagagcgatt tctcaatcgg gaaggagttt gaggatattc attcgtgggt ggagatacgc
    12961 ctgggtgata tggcagggga aggagcgggt gtgaatagag accgccaggg cacccgggat
    13021 gcccatagtg cggcaggtga gcttcatgca ggcagccccg gcgcgcagga tgcccgtagt
    13081 gcggcaggtg agcttcatgc agtcagccct ggtgcgc
//
LOCUS       DAOPBK010000064        13058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4753_length_13058_cov_5.018303, whole genome shotgun sequence.
ACCESSION   DAOPBK010000064 DAOPBK010000000
VERSION     DAOPBK010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13058
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4753_length_13058_cov_5.018303"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLA17_05275"
     CDS             <1..272
                     /locus_tag="PLA17_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84858.1"
                     /translation="DMMAGSPLFISLLALFLSVMMLLNVRMFSLKFTSLKWKGNEERL
                     IFVAVSILLLVIFRIAALPMIMAAYLLISFIFSVTAGRRTSSGVM"
     gene            complement(219..1388)
                     /locus_tag="PLA17_05280"
     CDS             complement(219..1388)
                     /locus_tag="PLA17_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HPK84859.1"
                     /translation="MITHIDNLETNAAVSMDIVQTGSWGFGEGPLIIAGPCSAESEEQ
                     LMATARALKATGRVHLLRAGLWKPRSRPSSFTGEGFRALEWLRRVKAETGLRVAVEVA
                     SPEHARAAIEAGVDALWIGARTVSNPFSVDTIASALRGSDIPVMVKNPLSPDLDLWEG
                     AIERVYSAGIRKIAAIHRGFTPYNKSRYRNLPQWELAIELRSRIPSLPVICDPSHMSG
                     KASLVPEMAQKALDMNMAGLMIEVHHDPASALSDREQQLTPDEFEKMMGSLLFRSHTS
                     SDIGFINHLEELRNRIDSIDYQLIGLLASRMRISGEMGEYKCRSGVTVFQLDRWLEIL
                     ESRTQQGVSEGLDIEFVGRVIRMIHEESIRCQDVVMKKLHDAGGCPPAGSDGENE"
     gene            complement(1372..2502)
                     /locus_tag="PLA17_05285"
     CDS             complement(1372..2502)
                     /locus_tag="PLA17_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPK84860.1"
                     /translation="MTLQISYLFLREYWWLIISLLGGLLVFLMFVQGGQSFIFSLPRN
                     DIQRKMVTYSLGRKWGFTFTTLVTFGGAFFASFPLFYATSFGGAYWLWMAILFSFVIQ
                     AVAYEYRSMPANVYGTRLFDTFLFINGCLGPFLIGVTVATFFTGSDFTLDLFNRVHWG
                     SGWHGLEALINPVNLLLGFAVLFLARVLGLMYLENSIDDKELTASIKKALVRNAVPFL
                     LFFLAFVAAILLGKGYRADPVTGEVTLVKYHYLHNLAGNVVTLVLFAGGVILVLISLW
                     LGIFRGSRRAIWYAGFGTVPVVIALFLMAGYGETAFYPSSTDPGSSLTLAKASSSYFT
                     LKTMMYVSFIIPFVVAYIWYAWIAITRSRITREELAGDDHTY"
     gene            complement(2515..4071)
                     /locus_tag="PLA17_05290"
     CDS             complement(2515..4071)
                     /locus_tag="PLA17_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPK84861.1"
                     /translation="MIENFDLSLVNWSRAQFALTAIYHWLFVPLTLGLSFILAFMETL
                     YVRTGDEMWKKITKFWMTLFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLA
                     VEGILAFFLETTFVAVMFFGWNKVGKKFHLASTWLVAIGASLSALWILVANAWMENPV
                     GMAFNPDTARNEMNSFREVVFNPVAVDKFLHTVTSGFVLASVFVVGISAWFLIRKREQ
                     ELARRSILIAGIFGLVSGLAVALTGDSSARTLAQVQPVKFAAMEALYEGKSDAGLTVI
                     GALTDSGEKIGEKKVHGMKLKIEIPGLLSIMTTGKGDGYVPGLKDLVNGNPERGILSV
                     SEMMERGGHARQVLIDYKMAKELGDTDSMAMLAARFEDKDFVENYFRYFGYSYFDHPW
                     QVIPSVPVTFYAFRVMVGLGFFFILLFVLAIWFHRRKTLEKNTWFLWIALFSIPLAYL
                     ASELGWIVTEMGRQPWIIQNLMPVHVAVTNISAGAVRTTFWLFAVLFTALLIAEISIM
                     VRQIKIGPKQ"
     gene            complement(4076..4303)
                     /locus_tag="PLA17_05295"
     CDS             complement(4076..4303)
                     /locus_tag="PLA17_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HPK84862.1"
                     /translation="MGSAAKTVNRIFRFYYDGFRTMSWWGKKVWIIILVKLFIIFLIL
                     RIFFFPDFLKVNFSNDRERSDYVLEQLTGNN"
     gene            complement(4303..5439)
                     /locus_tag="PLA17_05300"
     CDS             complement(4303..5439)
                     /locus_tag="PLA17_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPK84863.1"
                     /translation="MNTTLRNILIIIGLAILVYLFWYFRSMIAYILAAGVISLIGRPV
                     VDLLNGIRIWKFRFPKALSSLLTLLLLYGLFALFFAIFIPLVSRQIDALSGFDAGSIV
                     QGLRDQLDKIDTVLRKFYRDMPADKTLYDIVVTTITNILNPTSITDFAGNIVTVLRKM
                     VVGFVAITFLAFFFLKDEGLFKETIMVLVPDQYEKRVRNVLHSIKKLLIRYFIGIILQ
                     STVVLINITIGMTIIGFPFHQALVMGLLIGIFNIIPYVGPWLGGFAAVLMGVATAVTT
                     TGYPVIWLLIIYMVIVIACTQIIDNNLIQPMIYSRSVNAHPIEIFLVILAVGSFAGII
                     GMILAVPAYTALRVFAREFFYNFGPVRKITSGLGKEIRETEPEP"
     gene            5680..6108
                     /locus_tag="PLA17_05305"
     CDS             5680..6108
                     /locus_tag="PLA17_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPK84864.1"
                     /translation="MLPTITRRGYKPLTLSDFLNEDFFPTFTRSSTSLPAVNIREDEK
                     AFYLELAVPGMDKKNLKIEVKDEVLTISSEHNEEKHDEREGYMRREFSYSSFCRSFYL
                     PEDVNGDKIKANYKDGILNVEIPKLEEEKKKEKTRTISIS"
     gene            complement(6269..7486)
                     /locus_tag="PLA17_05310"
     CDS             complement(6269..7486)
                     /locus_tag="PLA17_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPK84865.1"
                     /translation="MKADNRVIDLTPDVKWIGVLDYDIRTFDIVMHTDHGTTYNSYFI
                     NAEKKAIIETAKEKFSDVFLSKLRQVTDPAELAYIVLDHTEPDHSGAMRQLLDLAPGA
                     VVVGSGNTIRYLGDIVNRPFKSLIVKDGDTLDLGNKTLKFISAPNLHWPDSIYTYIVE
                     DKILFTCDSFGAHYCHAELFDDLVGDYQADFRYYFDVILKPYSKFMVKAIEKIRPLEI
                     SMIAPGHGPVLRKNWKSIVDETRRLSEEYLALTERSGVKRVLITYVSAYGYTAEMARL
                     IAEGASKAENLEVEVLDIETADIGLIDSKIAIADGIIVGSPTINQNTLLPVYNLMAVV
                     SPLRDRGKLAGSFGSYGWSGEAPSMIAEMLKNLKLKYFEEPAAFKFVPEDGKDKTLIE
                     YGERFGKALTSPV"
     gene            complement(7498..8172)
                     /locus_tag="PLA17_05315"
     CDS             complement(7498..8172)
                     /locus_tag="PLA17_05315"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPK84866.1"
                     /translation="MTIGKFRIHREGYRIIIGVFLGLVALNIAGWLVWAGPTLYHWLA
                     AFASLLLFLFIVMFFRTPGRPLEPNPLLVYAPADGKIVVIEETYEKEYFRDNRLQISI
                     FMSPFNMHSNRYPISGTVTWTHYQPGKKFHARSPKSSELNERSSVVVSSENGTEILVR
                     QVAGAVARRIVTYSKKGDKVHQGDELGFIKFGSRVDIFLPTGTVVDVRMFEQVRASRT
                     ILASVI"
     gene            complement(8174..9016)
                     /locus_tag="PLA17_05320"
     CDS             complement(8174..9016)
                     /locus_tag="PLA17_05320"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPK84867.1"
                     /translation="MSNFVRRTVTGAFIVIFVLGGLWLHPVSFFLVGAVMIAGTQREY
                     YLMVRGTGVRPQLITGIISGFLLYVISTTVALGWVPRSWFLVMIPVMSIIMIIELFRN
                     VERPFDSLAHTFFSILYTAVPFSMFPFSAFNQTGLSPLIPMEGIVFSPGIMVGFFLLL
                     WTNDTGAYLVGSALGRHQLFKRISPKKTWEGFFGGLALTLLVARLFSGWLGVADTKGW
                     VVIALIISVGGTFGDLLESMLKRSLGLKDSGTILPGHGGFLDRFDSVVVAFPLVYLYI
                     ATML"
     gene            complement(9018..9215)
                     /locus_tag="PLA17_05325"
     CDS             complement(9018..9215)
                     /locus_tag="PLA17_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84868.1"
                     /translation="MENEWVVIAGFSDDVRSQIAVERLLSNGIDAVAVDKRDRIYKIG
                     EIEIFVHRDNVIIAKEIIKDL"
     gene            complement(9205..11337)
                     /gene="ftsH"
                     /locus_tag="PLA17_05330"
     CDS             complement(9205..11337)
                     /gene="ftsH"
                     /locus_tag="PLA17_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPK84869.1"
                     /translation="MAESNTPKSGKNEKSSGKSWFNSSWLFAILLLTFIVFQVLSSGK
                     YTRKAGQREIEEMIMHHDIEKVIIVNEEQAEIYLFPDSLKSGRYPDFAEQRNFGLQPP
                     KPHYYHTIGTVELFQKFFDEAQEKAGYTEDQRIYPEYQKRKDYLGTFLGFMLPIILIA
                     AIWIYFIRRMSSGAGGGAGGIFSVGKSKAQIFDKDTHIKINFNDVAGLEEAKTEVMEI
                     VDFLKNPKKYTSLGGKIPKGALLIGPPGTGKTLLAKAVAGEANVPFFSISGSDFVEMF
                     VGVGASRVRDLFKQAKEKAPCIVFIDEIDAVGRARGRNPNFGANDERENTLNQLLTEM
                     DGFGTNSGVIILAATNRADILDKALLRAGRFDRQIHVELPDLNEREAIFKVHIRPIKL
                     EDGFDISFLAKQTPGFSGADIANVCNEAALIAARKNKSVVQKQDFLDAIDRIIGGLER
                     KNKIISMEEKRTIAYHEAGHATVSWLLEHASPLLKVTIIPRGKALGAAWYLPEERSIS
                     TREHILDELAYALGGRAAEELVIGKKSTGALSDLEKVTKQAYAMVAYFGMSDEVGNIS
                     FYDSSGQNEYGFTKPYSERTAELIDREARKIVEDSYLKAKEIISANLDGLTQLAELLL
                     EKEVIFSEDLERIFGKRRGDKKTEDAAAEDAAADSSAQESGATGSPAREAADSSAQEG
                     GATGSPAREAETTAESTTTEVPKPGDGE"
     gene            complement(11345..11728)
                     /gene="rsfS"
                     /locus_tag="PLA17_05335"
     CDS             complement(11345..11728)
                     /gene="rsfS"
                     /locus_tag="PLA17_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPK84870.1"
                     /translation="MGKGRKESTGVTDSIVRGLLEKKGEKVALIDLRNIENRICDWFV
                     ISHAASTRLVDSLVWSVEDVVRRETGVKPCRIEGRENCIWVLLDYGDILVHIFQKPYR
                     EFYDLESLWADGRITFIDDNMVKKG"
     gene            11899..12639
                     /locus_tag="PLA17_05340"
     CDS             11899..12639
                     /locus_tag="PLA17_05340"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPK84871.1"
                     /translation="MIVGTYVRHFRKVSSTNSIAAAMLKEQAPAEGTVITASYQESGR
                     GQPGNSWESESGKNLIMSVILYPVMVRPADQFIISRMVALAVHDVVSKYTKNASIKWP
                     NDIYVNNDKIAGILIENSIMGETLSSSVAGIGLNVNQIVFRSGAPNPVSLKQVTGTTH
                     NLKAVKEEMMAALDRRYAMVSSGETAVLENEYQQLLYRAGEWHRYSDDNGEFEGMIER
                     VMTDGMLSVRRRDGTSRLYAFREIDYIL"
     gene            complement(12614..>13058)
                     /locus_tag="PLA17_05345"
     CDS             complement(12614..>13058)
                     /locus_tag="PLA17_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84872.1"
                     /translation="SPVTTFISRRVEVPCGDSDLYAFLTDMNNLRTVLPSGSVTDWEA
                     TEERCSFETDRAGRITVTRAEALPHSEISYEAESFLTGRVKVRITIGFISAMRSAVRL
                     DTGLEMNPLMRMLIGDSAGRYLDMLMDIIESYDGYDMIRGYNQSP"
BASE COUNT         3351 a         3500 c         3052 g         3155 t
ORIGIN      
        1 atgacatgat ggccggatca cctctgttca tcagcctcct cgctctcttc ctgtcggtaa
       61 tgatgctcct caatgtacgg atgttttcgc tcaagttcac cagcctgaag tggaagggaa
      121 acgaggagcg cctcatcttc gtggcggtaa gtatcctgct gcttgtcata ttcaggatcg
      181 cggcgctgcc aatgataatg gctgcctatc tcctcatttc attcattttc tccgtcactg
      241 ccggcaggag gacatcctcc ggcgtcatgt agcttcttca tcaccacatc ctggcagcgg
      301 atcgattcct catgaatcat ccgtattacc ctgcccacaa actcaatgtc aagaccctcg
      361 ctgacaccct gctgtgtcct cgactccagg atctccagcc accgatcgag ctggaagacg
      421 gtcacgccgc tccggcactt gtactctccc atctccccgg atatgcgcat cctcgatgcg
      481 agcagtccga tcagctgata gtcaattgag tctatcctgt tgcgcagttc ctcgagatga
      541 ttgatgaacc cgatatcact gctggtgtgt gaccggaaaa gcaagcttcc catcatcttc
      601 tcaaattcgt cgggagtaag ctgctgctcc ctgtcgctca gcgcactggc agggtcatgg
      661 tgcacctcaa tcatcagtcc tgccatgttc atgtcaagcg ctttctgtgc catttccggc
      721 accaggcttg ccttccctga catgtggctg ggatcacaga taacaggaag ggatggtatc
      781 cggctccgga gctcaatggc cagctcccac tgtggaagat tgcggtagcg gcttttgttg
      841 tagggggtga agcccctgtg aattgccgct atcttgcgta tgcctgcgct gtacactctc
      901 tcaatggctc cctcccacag atccaggtca ggactgagag ggttcttgac catcacgggt
      961 atatcacttc cccgcagggc tgaagcgata gtgtcaacgc tgaacggatt cgacactgtg
     1021 cgtgccccga tccacaacgc atcaacgcct gcctcaatgg cagcacgggc atgctcaggt
     1081 gatgccacct ccaccgccac ccggagccct gtctccgcct ttaccctgcg aagccactcc
     1141 agcgccctga acccctcacc cgtgaatgaa gagggccttg aacgcggttt ccataaaccg
     1201 gccctcagaa gatgaaccct gcccgtggct ttcagcgccc tggctgtggc cataagctgc
     1261 tcctcgctct cggcgctgca cggccctgca attattaaag gcccttcgcc aaaaccccac
     1321 gatcccgtct gcactatatc cattgacact gccgcgttag tttccagatt atcaatatgt
     1381 gtgatcatcg ccagccagtt cctccctggt tatccggctc cgggtgatcg cgatccaggc
     1441 ataccaaata tatgccacaa caaacggtat gatgaacgaa acatacatca tcgtcttcag
     1501 ggtaaagtaa ctcgatgatg cctttgccag cgtcagagag ctgccaggat cggttgatga
     1561 cggatagaaa gctgtctctc cgtagcctgc catcaggaac agggcaatta ctaccggtac
     1621 tgttccgaaa ccggcatacc atattgctct tcgcgaaccc ctgaagatac ccagccatag
     1681 tgatataagg accagtatca ctcctcccgc gaataaaacc agggtgacga cgttgccagc
     1741 caggttatgc aggtaatgat attttacaag tgtaacctct ccggtgaccg gatcggccct
     1801 gtaacctttc ccgaggagaa tagccgcgac aaatgcaaga aagaagagca ggaacgggac
     1861 ggcatttctc accagtgcct tctttattga agctgtcagc tccttatcgt cgatgctgtt
     1921 ttcaaggtac atcagcccca gcaccctggc caggaagagc accgcaaatc caagcagcag
     1981 gttaacagga ttgatcagtg cttccaggcc gtgccagcct gagccccagt gcaccctgtt
     2041 gaaaagatcg agggtgaagt cagagcctgt gaagaaggtg gccacggtga ctccgataag
     2101 gaacgggccc aggcagccgt tgatgaagag gaaggtgtca aagagacgtg tgccatagac
     2161 gttggcaggc atggagcggt actcgtaggc tactgcctgt atgacaaagc tgaacagtat
     2221 tgccatccat aaccagtatg cccctccgaa gctggttgca tagaagagcg ggaaagaggc
     2281 gaagaaggca ccgccgaatg tcaccagggt ggtgaaggtg aaaccccact tccggccaag
     2341 ggaataggtt accatcttcc gctgaatgtc gtttcgcgga agcgaaaaga taaacgactg
     2401 tcctccctga acaaacatga ggaaaacgag gaggcctccg agaagtgata ttatcagcca
     2461 ccagtactcg cgcaggaaca aataagatat ctgcaatgtc ataatccttt cctcctactg
     2521 ttttggtccg atttttatct gtcttaccat aattgaaatc tctgcaataa gcagggctgt
     2581 gaacagcact gcaaagagcc agaaagttgt ccggacagcg cctgcactga tatttgttac
     2641 ggccacatga acaggcatga ggttctgtat aatccagggc tgccggccca tctctgtgac
     2701 tatccagccg agctcactgg ccaggtaagc cagcgggatt gagaagagtg ctatccacag
     2761 gaaccaggta ttcttctcca gggtctttct ccggtggaac cagatggcaa gcacaaaaag
     2821 caggataaag aagaagccca gccccaccat aaccctgaag gcatagaatg tcaccggtac
     2881 agagggtatg acctgccagg gatgatcaaa atagctgtac ccgaaatacc tgaagtagtt
     2941 ttctacgaaa tccttatcct caaatctggc tgccagcatc gccattgagt cagtgtcacc
     3001 gagttccttt gccatcttgt agtcgataag cacctgcctt gcgtgacctc ctctttccat
     3061 catctcactg acagagagaa tacctctttc aggattgccg ttaaccaggt ccttcagccc
     3121 ggggacgtaa ccgtcgccct tgcctgtagt catgattgag agcagcccgg ggatctcaat
     3181 cttcagcttc atgccatgta ccttcttctc gccaatcttc tcgccgctgt cagtaagtgc
     3241 accgataacc gtcaggcctg cgtcgctctt tccctcatac agagcctcca ttgcagcaaa
     3301 tttaaccggc tgcacctgtg ccagggtacg tgccgaagag tctcctgtca gcgccaccgc
     3361 cagccccgat accaggccga atatgccggc aatgagtata ctccttcgtg ccagctcctg
     3421 ctctctcttc cttatcagga accaggcact tatgccaaca acaaaaaccg atgccagaac
     3481 aaatccggat gtcaccgtgt gcaggaattt atctactgca acaggattga agaccacttc
     3541 gcggaagctg ttcatctcat tgcgtgcggt atcaggatta aaagccatgc caaccgggtt
     3601 ttccatccat gcattggcaa ccagtatcca gagggctgaa agactagcgc caatggcgac
     3661 aagccaggtg gaggccaggt ggaatttttt gcccaccttg ttccagccga aaaacatgac
     3721 agccacgaag gttgtttcaa ggaagaaggc caggatgcct tcgacagcca gcggcgcccc
     3781 gaagatatcg cccacaaacc acgaatagtt cgaccagttg gtgccaaatt caaattcaag
     3841 tatgatgccg gtggccaccc ctatggcgaa gttgatgccg aagagtgtca tccagaattt
     3901 tgttatcttc ttccacattt catcaccggt gcgcacatag agagtctcca tgaaggcaag
     3961 aataaatgag aggccgagcg tcagaggcac gaagagccag tgatagattg ctgtcagtgc
     4021 gaactgagcc cttgaccagt ttacaagtga caggtcaaag ttttcaatca tattgtcagt
     4081 tgtttccggt taattgttca agtacataat cactcctctc cctgtcatta ctgaagttaa
     4141 ccttcagaaa atcagggaag aaaaatatcc tgagtattaa aaaaatgatg aatagcttga
     4201 caaggattat tatccagacc tttttccccc accatgacat ggtccggaaa ccgtcataat
     4261 agaatctgaa tatcctgttg actgttttgg ctgcgctgcc cattatggtt ccggctctgt
     4321 ttctctgatc tcttttccca gacctgatgt aatctttctt accggtccga agttatagaa
     4381 gaactcacgt gcgaagaccc tgagagcggt ataggccggc accgccagaa tcattccgat
     4441 gattcctgca aagctgccaa cggccaggat taccaggaag atctcaatcg gatgggcatt
     4501 gacgcttcgc gaatagatca tcggctggat aaggttgtta tcaatgatct gggtacaggc
     4561 aatgacgata accatataga ttataagcag ccatatcaca ggataacctg ttgtggtcac
     4621 cgcggtggcc acacccatga gaactgcagc gaaaccacca agccaggggc ctacataggg
     4681 gatgatgttg aagatcccta tcagcagccc catcaccagc gcctggtgga acgggaaacc
     4741 gataatggtc ataccgatgg tgatgttgat aagcaccacg gtgctctgaa gaatgatacc
     4801 tatgaaatac ctgatgagca gtttcttgat tgaatgaagc acattcctga cccgcttttc
     4861 atactgatcc ggcaccaaca ccatgatcgt ctccttgaac agcccctcat ccttcaggaa
     4921 gaagaatgcg aggaaggtta tagccacgaa gccgacaacc atcttgcgca atacggtaac
     4981 aatattcccg gcaaaatcgg taatggaggt cggattgaga atgttggtta ttgttgtgac
     5041 aacaatgtca tacagggttt tgtcagccgg catatcccgg taaaactttc tcagtaccgt
     5101 atcaatcttg tcaagctggt cacgaagacc ctgcacaatg gaaccggcgt caaaaccgga
     5161 aagggcatca atctgacggc tcaccagcgg gatgaaaatg gcaaagaaga gtgcaaagag
     5221 cccatagagc aacagtagag tcagcaacga gctgagtgcc ttcgggaatc tgaatttcca
     5281 gatacggata ccattcagca gatcaactac cggcctgcca attagtgaga tcaccccggc
     5341 agcgaggata taggctatca tactcctgaa ataccagaaa agataaacca atatagccag
     5401 tcctataatt atgaggatgt tacggagggt agtattcatt gtcaaacagg tctcaagcaa
     5461 ttggttttcc gtacttcacc gtgcgggcaa acattgcatt ttcaaaatta gaaataattc
     5521 ccggtttaac aacctgatca tataaaatgc cattatgtca tgctaaatat tgattgcatg
     5581 ccattgtgtc atctttttgg aagcggcaca tgaattgaaa tattaagggt gtaaaagcaa
     5641 ataaaaataa agtctaataa tataaaaaag gaggttacta tgttaccaac gattaccaga
     5701 agaggttaca agccgcttac tttatcggat tttctcaatg aagatttctt tcccacattc
     5761 accaggtcat caaccagttt gcctgcagtg aatatcaggg aagatgagaa ggcgttttat
     5821 cttgagcttg ccgttcccgg catggacaag aagaacctga agattgaagt aaaagatgag
     5881 gttctcacca tttcttcgga gcataatgag gagaagcatg atgagcggga gggttacatg
     5941 cgcagggagt tcagttacag ctcattctgc cgcagtttct atcttcccga ggatgtaaac
     6001 ggagataaga ttaaagccaa ctacaaggat ggcatcctga acgttgagat tcccaaactt
     6061 gaagaagaga agaagaagga aaagaccagg acaatctcaa tctcgtaaca tccacaacat
     6121 tgcatcatag tgccgggtct gagacccggc actaccatta tgtccggatg attctgcatg
     6181 catgcacgtg gcgccgggtc tcagattctg catgcatgca cgtggcgccg ggcctcagac
     6241 ccggcgttac catatataat atccaatgtt atacaggaga tgtcaatgcc tttccaaacc
     6301 gctcaccata ttcaatgagc gttttgtcct taccatcctc gggaacaaac ttaaaggcgg
     6361 ctggctcttc gaaatatttc aatttcaggt tcttcagcat ctccgcaatc atcgatggcg
     6421 cctcaccact ccagccgtat gatccgaatg accctgccag ctttcccctg tcacgcaacg
     6481 gactgacaac agccatgaga ttgtagaccg gcagaagggt gttctggttg atggtaggcg
     6541 atccgactat gatgccatct gctatggcaa tcttcgagtc aatcaatccg atatcagccg
     6601 tttcaatgtc cagaacctcc acctccaggt tttctgcctt tgaagcgcct tcagctatca
     6661 acctggccat ctccgctgtg tatccgtatg ccgagacata tgttatcagc actctcttca
     6721 cgcctgaccg ctcagtaaga gcaagatact cttctgataa acggcgggtt tcatctacga
     6781 tactcttcca gttcttcctg agaacagggc catggccggg ggcaatcatt gaaatctcaa
     6841 gcgggcgtat cttctctatg gccttgacca tgaacttgct gtaaggcttc aggatcacat
     6901 cgaaataata cctgaaatct gcctgataat cacccacaag atcgtcaaac agctccgcat
     6961 ggcagtaatg cgccccgaat gaatcacatg taaacagtat cttatcttcc acaatgtatg
     7021 tatagatgct gtcaggccag tgaaggttcg gagccgagat gaacttcagt gtcttgttgc
     7081 ccaggtcaag tgtatcacca tctttcacta tcagtgattt gaaaggcctg tttactatgt
     7141 caccgagata cctgattgtg ttgccgcttc caaccaccac cgccccggga gccagatcaa
     7201 gcaactgacg catggcgccg gaatggtcgg gctcagtatg atcaaggact atgtatgcca
     7261 gttcagcagg atcagtgacc tgccggagct tgctgagaaa aacatcactg aatttctcct
     7321 tggctgtttc tattatggcc tttttctcag cgttgatgaa ataggagtta taggtagtgc
     7381 cgtgatcagt atgcatcacg atatcaaaag tcctgatgtc ataatcaagc actccaatcc
     7441 attttacatc aggagtaaga tcaattacac ggttgtctgc tttcattttt ttaaacatta
     7501 aataacactt gcaagaattg ttctgctggc cctgacctgt tcaaacatcc tgacatcaac
     7561 caccgttccg gtgggaagga agatatctac gcgcgagcca aacttgatga accccagttc
     7621 atcgccctga tgcaccttat ctcccttttt cgagtaggtc actatgcgac gggccacggc
     7681 acctgccacc tgcctgacaa gaatctcagt gccgttctca ctggaaacaa ccacactgct
     7741 cctttcgttc aattcggatg atttcgggct tcgggcatga aattttttgc ccggctggta
     7801 gtgtgtccag gtaacggtac ctgagattgg atacctgttg ctgtgcatgt tgaagggcga
     7861 catgaatata gagatctgaa ggcgattgtc cctgaaatac tccttctcat atgtctcttc
     7921 aataaccaca atcttaccgt cagccggtgc atagacgaga agagggttgg gctcaagcgg
     7981 tctgcccggt gtcctgaaaa acatcacgat gaaaagaaac aggaggagtg acgcaaaagc
     8041 ggccagccag tgataaagcg tagggccggc ccacaccagc catccggcga tgttcagggc
     8101 aaccagccca agaaacacac ctataattat cctgtaaccc tccctgtgta tcctgaattt
     8161 tccaatagtc atatcaaagc atcgttgcta tgtaaagata aaccagcggg aatgccacta
     8221 ccacgctgtc aaacctgtca agaaagccgc cgtgtcccgg caaaattgtt cctgagtcct
     8281 tcaatcccag gctccgcttg agcatcgact ccagcaggtc accaaacgtt ccccccacgg
     8341 agatgatcaa tgcaatgacc acccaccctt tggtatcagc cacgccgagc cacccggaaa
     8401 agagccttgc cacgagcaat gtcagcgcca ggccgccaaa gaaaccttcc catgttttct
     8461 ttggtgatat acgtttaaag agctggtgcc gccccagggc agaccctaca agataggcac
     8521 cggtatcatt ggtccatagc agcaggaaaa aaccaaccat gattccgggt gagaatacaa
     8581 tcccctccat cggtatcagc gggcttaagc ctgtctggtt gaaagcagag aagggaaaca
     8641 tggaaaacgg cactgcggtg tacagtatcg agaaaaaagt atgtgccagg gaatcaaaag
     8701 gtctttccac gtttcggaac aactcgatga tcattattat tgacatcacc gggatcatta
     8761 caaggaacca gcttctcgga acccacccca gcgccactgt ggtggagatg acatagagaa
     8821 ggaatccact gattattccg gtgatcagtt gcggccgtac ccctgtaccc ctgaccatga
     8881 ggtaatactc acgctgtgtg ccggcaatca tcactgcacc aacaaggaag aatgatacag
     8941 ggtggagcca caatccacca agtacgaaaa tcactatgaa ggcaccggtg acggtgcgtc
     9001 tgacaaaatt tgacacacta caaatccttg attatttcct tggctatgat cacattgtca
     9061 cgatggacaa aaatctcgat ctcacctatc ttatatatgc ggtcacgctt gtctaccgcc
     9121 actgcatcga ttccgtttga cagaagcctc tcaacggcaa tctgacttct gacatcatcg
     9181 ctgaagcctg ctatgacaac ccactcattc tccatcaccg ggctttggga cttcagtagt
     9241 tgtggactca gcagtggttt cagcttcccg ggccggtgat ccggtagctc cgccctcttg
     9301 tgcagatgag tctgcagcct cccgggccgg tgatccggta gctccgctct cttgtgcaga
     9361 tgagtctgca gcggcatcct ccgcggcggc atcctcagtc ttcttgtcac cacggcgctt
     9421 gccgaagata cgctcaagat cctcactgaa gatgacctcc ttctcaagca acagctcggc
     9481 aagctgtgtc aacccgtcta aattggccga gatgatctct ttagccttca gatatgaatc
     9541 ctcgacaatt ttccttgcct ctctgtcaat cagctcggct gtcctctcac tgtacggttt
     9601 ggtaaagccg tactcattct ggccggagga gtcatagaaa ctgatattac ccacctcatc
     9661 agacatgccg aagtaggcca ccatggcata tgcctgcttg gtaaccttct ccaggtcact
     9721 cagtgcaccg gttgatttct ttccaatgac cagctcctca gcggctcttc cgccaagggc
     9781 ataggccagt tcgtcaagta tgtgttcacg cgttgagata gacctctcct ctggcagata
     9841 ccacgctgcg cccagcgcct tgccccttgg gatgatggtc accttgagca acgggctggc
     9901 atgttcgaga agccagctga cagttgcatg accggcctca tggtaggcaa tggtcctctt
     9961 ctcctccatg gagatgattt tgttctttct ctccaaccct ccgatgatac ggtcaatggc
    10021 atcaaggaaa tcctgctttt gaaccacact cttgttcttc ctcgcggcaa taagcgcagc
    10081 ctcattgcac acgttggcaa tgtcagcccc tgagaatccc ggagtctgct tggccaggaa
    10141 ggagatgtca aacccatcct caagtttgat cgggcggatg tgcaccttga aaatggcctc
    10201 acgttcattc aggtcaggaa gctccacgtg tatctgccgg tcgaaacggc ctgccctgag
    10261 cagagccttg tcaaggatat cagctctgtt ggtggctgca aggataatca ctccgctgtt
    10321 ggtaccaaac ccgtccatct cggtaagcag ctggttaagg gtattctccc gctcatcatt
    10381 ggcgccgaaa ttggggttac ggccacgggc acgccctaca gcatcaatct catcaatgaa
    10441 gacaatacag ggagccttct ccttggcctg cttgaacagg tcccttaccc tcgaggcacc
    10501 cacacccaca aacatctcca cgaagtcaga ccctgaaatg gagaagaaag gaacgttggc
    10561 ttcaccggca acagccttgg ccagcaatgt ttttcccgtt cctggaggac cgataagcaa
    10621 agctcccttg ggtatcttgc cacccagaga ggtgtatttt ttcggattct tgagaaaatc
    10681 aactatctcc ataacctctg tcttggcctc ctcaaggcct gccacgtcat tgaagttgat
    10741 cttgatatgg gtatccttgt caaatatctg ggcttttgat ttcccgacac tgaagatgcc
    10801 accggcaccg ccaccggcac cggaagacat gcggcgtatg aaatagatcc agatagcggc
    10861 tatcagaatg atcggaagca tgaacccaag gaacgttccc aggtagtcct tccttttctg
    10921 atattcagga tatatgcgct gatcttccgt ataaccggcc ttctcttgtg cctcatcaaa
    10981 aaacttctga aaaagctcaa cagtgcctat ggtatgatag taatgtggct tgggcggctg
    11041 cagtccgaaa ttccgttgct ccgcaaaatc aggataacga ccgctcttga gggagtcagg
    11101 gaagagatat atctccgcct gttcctcgtt gactatgatc accttctcga tgtcatggtg
    11161 catgatcatc tcctctatct ccctctggcc cgccttccgg gtatacttgc cggaactgag
    11221 gacctggaaa acgatgaatg tcagcaacag aatcgcgaac agccagctgg aattaaacca
    11281 tgacttgccg ctactctttt cattcttgcc tgacttggga gtattgcttt ctgccataaa
    11341 taaatcatcc tttctttacc atgttgtcat cgataaacgt tatgcggccg tcagcccata
    11401 acgactcgag gtcataaaat tccctgtaag gcttctggaa gatatgcacc agaatgtcac
    11461 cgtagtcaag caatacccag atgcagtttt ccctgccctc aatgcggcag ggctttaccc
    11521 ctgtctctct cctgaccaca tcctccactg accatacaag tgaatcaacc agtctggttg
    11581 aggcggcgtg actgataacg aaccagtcac aaatcctgtt ttcaatattc ctcaaatcta
    11641 tcagagccac cttctcaccc tttttctcca gcagacccct gacaatgctg tcggtcaccc
    11701 cggtggactc cttcctgcct tttcccatca ataatctttt cagttataaa cagttctgta
    11761 agcagatgca aaattacaaa catttatccg tattttggtc cgcctttagt tcttttcaca
    11821 atttgggtgc caatttgatc agaacggtta ttactgccgc tttccgtcag aaaagcaccc
    11881 ctttgtcaga tgattgtcat gatagtaggt acatatgtca ggcacttcag gaaagtgtca
    11941 tccacgaact caattgcagc tgccatgctg aaagagcagg ctcctgccga aggtacagtg
    12001 ataaccgctt cataccagga gagcggcagg ggacagcctg gcaacagctg ggagagtgag
    12061 tcaggcaaga atttgattat gagtgtaata ctttacccgg tcatggtccg gccggctgat
    12121 cagttcatca tttcaaggat ggtcgcgctg gcagttcacg acgtggtatc gaaatatacg
    12181 aagaatgcga gcatcaagtg gcccaatgac atctatgtga ataatgacaa aattgcaggc
    12241 attctgatcg agaacagtat catgggcgag actctcagca gcagtgtagc gggcatcgga
    12301 ctcaatgtaa accagattgt cttcaggagc ggtgcaccaa atcctgtctc actgaagcag
    12361 gttacaggca ccacccataa tctcaaggct gtgaaagagg agatgatggc tgcccttgac
    12421 aggaggtacg cgatggtcag cagtggagaa actgccgtgc tggaaaatga ataccagcag
    12481 ctcctctacc gggccgggga atggcaccgc tattcagatg acaacggcga gtttgaaggg
    12541 atgatagaga gagttatgac tgacggcatg ctctccgtca ggagacggga cggcaccagc
    12601 cggctgtatg ctttcaggga gattgattat atcctctgat catgtcataa ccgtcgtatg
    12661 actctatgat gtccatcagc atgtcaaggt atcgtcctgc cgaatcacca atgagcatcc
    12721 tcatcagggg gttcatctcc agcccggtgt caagacgaac tgctgatctc atcgcgctga
    12781 tgaatccgat ggtgatcctc acctttaccc tgccggtgag gaacgactct gcctcatagg
    12841 atatctccga atgaggcaat gcttctgccc gtgtcacggt gattcgtcct gccctgtcag
    12901 tttcgaaact gcacctctct tcagtggctt cccagtctgt gactgaaccg gaaggaagta
    12961 ccgtacgcaa gttattcata tctgtcagga acgcgtagag gtcactgtct ccacatggta
    13021 cctctaccct cctgctgata aaagtggtca ccggactc
//
LOCUS       DAOPBK010000065        12921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4812_length_12921_cov_5.158946, whole genome shotgun sequence.
ACCESSION   DAOPBK010000065 DAOPBK010000000
VERSION     DAOPBK010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4812_length_12921_cov_5.158946"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..707)
                     /gene="purN"
                     /locus_tag="PLA17_05350"
     CDS             complement(135..707)
                     /gene="purN"
                     /locus_tag="PLA17_05350"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPK84873.1"
                     /translation="MKNIAIFASGAGTNAENIIRYFSTEKKAKVTLVLSNKPQAMVLK
                     RAAALGVDAFFFDRSQFYTTGEVLMMLRRRETHIVVLAGFMWLVPGDIIDEYRGRIVN
                     IHPALLPGFGGKGMYGDRVHRAVLDAGCTESGITIHYVNEHYDSGDIILQVRCPVLPG
                     DDVNTLASRIHALEYKHYPEVISRLVDELE"
     gene            893..1129
                     /locus_tag="PLA17_05355"
     CDS             893..1129
                     /locus_tag="PLA17_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPK84874.1"
                     /translation="MSDISTRVKKIIVEKLGVDETEVTPEAHFTNDLGADSLDQVELI
                     MEFEKEFNIGIPDDQAESISTVGEAIKYIEENAK"
     gene            1150..2397
                     /gene="fabF"
                     /locus_tag="PLA17_05360"
     CDS             1150..2397
                     /gene="fabF"
                     /locus_tag="PLA17_05360"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPK84875.1"
                     /translation="MRRVVVTGMGALTPIGNNLQDFWKNLVDGVSGAELITRFDPSRF
                     KTRFACEIKEYDPSRFLDRKESRKMDRYTQYAHIAADEAVKDAALDPGAIDKDRCGII
                     WGSGIGGIETFLDEVKGFVTGDGTPRFSPFFIPKMISNIAAGMLSIKYGFRGPNFGTV
                     SACSSSTNALIDAYYYIKLGKADLFIAGGSEASINPAGIGGFNAMQAMSTNNDEYATA
                     SRPFDVTRDGFVIGEGAGTLILEELGHARARGARIYAELAGSGLTADAYHITAPHPDG
                     IGAVNVMKQAIDEAGLKPSDVDYINVHGTATPLGDIAEIKAINALFGEHACKLNISST
                     KSMTGHLLGAAGAVEAIATIMAIVHGIVPPTINFRNPDPAIDQNLNLTLNKAQQRTVN
                     VALSNNFGFGGHNASILIRKFIE"
     gene            2397..3134
                     /locus_tag="PLA17_05365"
     CDS             2397..3134
                     /locus_tag="PLA17_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HPK84876.1"
                     /translation="MFLLNRKKSNTLIHDRDKFRSELKNLLGFRPRNLRLYEMAFIHR
                     SATYILPDGVRVNNERLEYLGDAIIDSILSDYLFHLYPEAAEGFLTKTRARIVNRETL
                     NQLGLSMGLDRLIVSNLAPSDSPRNLYGNAVEALVGALFIDRGYDRTRKFLIERGLKR
                     HFNLSALLAAETDFKSMILEYCQKKKQKLNFTSREKQSGGNGHPIFTVTLEISNKVIS
                     QGEGSTKKDAEQVASMAALNKLGLLKG"
     gene            3131..3559
                     /locus_tag="PLA17_05370"
     CDS             3131..3559
                     /locus_tag="PLA17_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HPK84877.1"
                     /translation="MSREKKITRHTIGTGEPQPFFFLGVVSAEPDYRLSVMINRHLGT
                     DLRKCNEDITVAGPAGTQQFSRFSPENRAFSLVSNRSGVNVLLRKLKNIDYLLVPGGV
                     QGKKEAEELAASLRLIPEITAVFIFDSRHNSDQNLSLLAL"
     gene            3831..5369
                     /locus_tag="PLA17_05375"
     CDS             3831..5369
                     /locus_tag="PLA17_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPK84878.1"
                     /translation="MKRSYIVLLAIFFFLAISALVIIQVRWINNVVSAEDQQFRFGVN
                     EALKEVVSELEKAETYKRIMSDINPEPTPQETDETSDQSEQKSAEEKLLEKYGFDPGV
                     RSVIISRAGYTYLLDSDSLEVESFFESAEPDGQILTAGATSRMTSKIISIEKIVSKIL
                     HETPPLRDRFTADELNNLIRRSLDAVGIHLEYECSVRGAYGDLIYITPNFQGSSGANK
                     YLRQLFPNDPVPGNNILTIYFPEEEEYKFSQIAFMASSSMLIVLLLTLLATGTFIVIF
                     RQKRLSEIRSDFINNMTHELKTPIATISLASQMLDDPSIPDSSRNIGSIATVIREESN
                     RLKVLVERVLHTAIFEKAKLKLNKQPVDIHSLILRSVDAFKLQVNGRGGTISTFLDAS
                     DPVVAIDEANIFNVMLNLIDNALKYTLDTPEITITTATWNRGIIITVSDNGIGIPREH
                     LKHVFEKFYRVPAGNTHNIKGFGLGLSYVKKIIEEHYGTIKVESQTAKGTRIIIHLLK
                     EPPK"
     gene            5366..6052
                     /locus_tag="PLA17_05380"
     CDS             5366..6052
                     /locus_tag="PLA17_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPK84879.1"
                     /translation="MKKISILLAEDDPNLGQLLKNYLIAREYDTTLVTDGAQAMQIYR
                     KERYQLCILDVMMPELDGFTLAKKIRALDPHIPIIFLTAKNLKEDVIEGFKSGADDYL
                     TKPFSMEELLYRIEAILRRASGKMAEPPASSYTIGKFTFDVTKQLLIFNDQSRKLTTK
                     ESELLELLCRHRNEVLERNFALKSIWIDDNYFNARSMDVYITKLRKYLSRDESVEILN
                     IHGKGYKLLC"
     gene            complement(6399..8186)
                     /gene="lepA"
                     /locus_tag="PLA17_05385"
     CDS             complement(6399..8186)
                     /gene="lepA"
                     /locus_tag="PLA17_05385"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPK84880.1"
                     /translation="MEYIRNFCIIAHIDHGKSTLADRLLEKTSTVDERDFHDQVLDNM
                     DLERERGITIKSHAIQMSYQYEGTEYSLNLIDTPGHVDFSYEVSRSIAACEGALLVVD
                     ATQGIQAQTISNLYMAIEHGLEIIPVINKMDSPSAMPEEVKDQIVDLIGCSREEIIEA
                     SARTGMGIDRIMSEIITRIPAPKGDPDAPLQALIFDSVFNSYRGIIAYFKIINGTIRT
                     HEMVKFVNTGSEYDADEIGVLKLKREPRDVLRAGDVGYIISGIKVSAEVKVGDTITHV
                     ARPCDKAISGFADVKPMVFAGIYPVDADDYEDLRASIEKLQLNDASLTFVPESSAALG
                     FGFRCGFLGLLHMEIIQERLDREFDMDVITTVPNVSFRVLTTKGEIIDVHNPSGMPPA
                     TTIDEIEEPYIHAQVISKSEYLGAILKLCIDKRGTMKNQVYLTSDRVEVTFDMPLSEI
                     VFDFYDKLKSISKGYASFDYHITGYKKADLVRLDILLNGEMVDALSTLIYRGHAYDFG
                     RRMCEKLRELIPRQQFDIAIQAAIGAKIIARETVKAVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGNVEVPQQAFLAVLKFD"
     gene            complement(8501..9904)
                     /locus_tag="PLA17_05390"
     CDS             complement(8501..9904)
                     /locus_tag="PLA17_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPK84881.1"
                     /translation="MTDPRQVYELEHEKPGRLMWKYFLPAFASMMANALYNVVDRIYI
                     GQGVDALALSGLTVVFPLMIVFMAFGMLVGMGSAVRISLALGEKDFNRANRILGNAFF
                     LSLVLGLVLMGAGFLVRDRVLQIFGAGPETIKYASDYFTIILLGTPLATVGFSLNNVI
                     RAEGNPRIAMYSIFISAGLNIILDPIFIFALDMGVKGAAIATVISHLVLAVWVLLHFR
                     SKRSVTRLTAATFRPDFYIIRHIISIGFAPFAMNLASSIIWGVMNTKFIRYGGDISVA
                     ALGIVNSATMLLVISIISINMAVQPIIGFNYGAGLFCRVKETIMKAIRYATLIAVGGW
                     LICMLAPGVIISIFNTDSPELREAGTLGLRIYCAALPVVGFQIIASNYFQAIGKARLA
                     IFLSMLRQIIILFPLIIILPEFWGASGVWVAIPVSDFVAAVISFILFRREIAKLECDM
                     PVAGRKNPEIAPPPETC"
     gene            complement(9992..11626)
                     /gene="groL"
                     /locus_tag="PLA17_05395"
     CDS             complement(9992..11626)
                     /gene="groL"
                     /locus_tag="PLA17_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPK84882.1"
                     /translation="MSKEIKFNIEARGLLKEGVDRLADAVKVTLGPKGRNVVLEKKFG
                     APQITKDGVTVAKEIELSDPYQNMGAQMVKEVASKTSDTAGDGTTTATVLAQSIISVG
                     LKNVTAGANPMDLKRGIDKAVATVVESIKNQSQSVGEDLKKIEQVARVSANNDDEIGK
                     LIATAMDKVKKEGVITVEEAKGTETKVEVVEGMQFDRGYISAYFVTNPDKMEVEMENP
                     YILIHDKKISSMKDLLPILEETARSGRGLMIIAEDVEGEALATLVVNRLRGSLKVCAV
                     KAPGFGDRRKEMLEDIAILTGGTVISEERGMKLENTTMADLGVADKVTVNKENTTIVN
                     GAGEKEMIQARINQIKQQISTTTSDYDKEKLQERLAKLAGGVAVLYIGAASEVEMKEK
                     KDRVDDALHATRAAIDEGIIPGGGVAYIRAIPLLEKLKGDNDDQNTGIDIVKRAIEEP
                     LRQIVTNAGLEGAVIVQKVREGKDDFGYNAHTDKFENLMKAGVIDPAKVARIALENAA
                     SIAGMFLTTEAVIVEEKEENPMPNPAMGGGGMPGMM"
     gene            complement(11647..11925)
                     /locus_tag="PLA17_05400"
     CDS             complement(11647..11925)
                     /locus_tag="PLA17_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPK84883.1"
                     /translation="MAQLKGKVLAGKVLVKPQEAEEKTASGIIIPDSAKEKPRAGTVV
                     LVGAAKKDEEMELKKGDVVLYGKYSGQELNIDGTDYLLLSQSDVLYIS"
     gene            complement(12058..12408)
                     /gene="secG"
                     /locus_tag="PLA17_05405"
     CDS             complement(12058..12408)
                     /gene="secG"
                     /locus_tag="PLA17_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPK84884.1"
                     /translation="MGIYIFLTILVIIACALVILVVLVQNSKGGGLAANFTSAGQSMG
                     VRKTADFLEKSTWTLGVAILVLCLAATASIPRGQGAKESVIKSSIERAQDPNAIPVLP
                     TQTPVQGQPDGGNE"
     gene            complement(12416..>12921)
                     /locus_tag="PLA17_05410"
     CDS             complement(12416..>12921)
                     /locus_tag="PLA17_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84885.1"
                     /translation="LELIPDEPVLEYEPAAQPVPDELVLEYEPAAEPVPDEPVVETES
                     ATQLTPDEPPEEAEPAAQLTPDGPPEEAEPAAQLTPADLIDRFIRTSPSIERLSPDDL
                     QPVKDLSAESTEEHGKFITETLAKIYINQGYYSRAINIYEKLSIQYPEKSVYFASRIE
                     KIKDLIK"
BASE COUNT         3246 a         3284 c         3070 g         3321 t
ORIGIN      
        1 tttctgccag ttagtcgggc tgagtttgcg tccgtgactc tggctgagtt tctgtctggt
       61 gagtggacga gggtttgtgt ccgtgactct ggctgagttt ctgtctggtg gccgggcgag
      121 ggggcgcgcc accctcattc cagttcatca accagtctgc tgatcacctc gggatagtgt
      181 ttgtattcca gggcatggat acgtgatgca agggtattta catcatctcc cggcagaacc
      241 gggcagcgta cctgcagaat tatatcacct gaatcatagt gctcattcac gtaatggatg
      301 gttatgccgc tctctgtgca tccggcatcg agtaccgcac ggtgaaccct gtcgccatac
      361 attccctttc caccgaaccc gggcagaagg gcagggtgaa tgttcacaat ccgtccacga
      421 tactcgtcga taatatcacc cggaaccagc cacatgaacc ctgccagaac cactatatgg
      481 gtttccctcc tgcgcagcat cataagcacc tctccggtgg tgtaaaattg agaccggtcg
      541 aaaaaaaatg catccactcc caatgccgca gcccttttca ataccatagc ctgaggctta
      601 tttgacaaaa cgagggttac ttttgctttt ttttcggtcg aaaagtatct gatgatgttt
      661 tcagcgttgg tcccggcacc tgaggcaaat attgctatat tcttcatatt ctgatactta
      721 gtctgttttt cggttcgggc cggcggagct caattttgag tcttaaaata ctgaatattt
      781 gtgacttatg tctgtcaaga tacaaatttc cggtaaattc cttgaatttt atggtacaaa
      841 tatatttctt tgcagagttt taaaagtgag tattaaccta aaattgacta gtatgtctga
      901 catttccaca agggtaaaga agatcatcgt tgaaaagctt ggcgttgatg aaacagaagt
      961 tactcccgaa gcacatttta caaatgacct tggtgcggac tctcttgacc aggtagagct
     1021 gatcatggaa tttgaaaagg agttcaatat cggaattcct gatgatcagg cagaaagcat
     1081 cagcacagta ggcgaagcca tcaagtatat tgaagagaac gccaagtagt tatctctggt
     1141 aacccataaa tgaggcgtgt agtagttacc gggatggggg cgctgacccc catcgggaac
     1201 aatctgcagg atttttggaa aaatcttgtt gatggtgtca gtggtgctga gttgataacg
     1261 cgttttgacc cgtccaggtt caaaaccagg tttgcctgtg agatcaagga gtatgatcct
     1321 tcccgttttt tggacaggaa ggaatcgcgc aaaatggacc gctataccca gtacgcccat
     1381 attgctgctg atgaggcagt aaaagatgca gctcttgatc ccggggcaat cgacaaggat
     1441 cgttgcggta taatctgggg ttcaggcatt ggcggcatag agacttttct tgacgaggtg
     1501 aaggggtttg ttacgggcga cggcacgccc cgtttcagtc catttttcat tcccaagatg
     1561 atcagtaaca tcgctgccgg aatgctctca atcaagtatg gtttccgcgg gcccaacttt
     1621 gggactgtct cagcgtgttc ttcttctacg aatgcactca ttgacgcata ctattacata
     1681 aagcttggca aggctgatct gttcattgca ggaggctcgg aggcgtcaat caatccggca
     1741 ggcataggcg ggttcaacgc catgcaggcc atgtcaacaa acaatgatga gtatgccact
     1801 gcctcccgcc cctttgacgt cacccgtgat ggctttgtca ttggcgaggg tgccggcacc
     1861 ctcatactag aggagcttgg ccatgccagg gccaggggcg cacgcatcta tgcagagctg
     1921 gcaggttccg gcctcacagc tgacgcctat catattactg ctcctcaccc tgacggcata
     1981 ggagctgtca acgtgatgaa acaggctatc gatgaagccg gtctgaaacc ctctgacgtt
     2041 gactatatca atgttcacgg cactgccacc cctcttggtg acatagctga gataaaagcc
     2101 atcaatgctc tctttggcga acatgcctgc aaactgaaca tcagctcaac aaaatcgatg
     2161 accggccacc ttcttggtgc cgccggggca gttgaagcca tagcaacaat catggcgatt
     2221 gttcatggca tcgttccgcc aacaattaac ttcaggaatc ctgatcctgc aatagaccag
     2281 aacctgaacc tcaccctgaa caaggctcag cagagaacag taaacgtggc tctcagcaac
     2341 aacttcggct tcggggggca caacgcatca atcctgatcc ggaaattcat tgaatagtgt
     2401 tcctgcttaa caggaagaaa agcaacacac tcatccatga ccgtgacaaa ttccggtcag
     2461 agctgaagaa ccttctcggg ttccgtccgc gtaacctcag gttgtatgag atggccttca
     2521 tacaccgttc cgcaacctat atcctgcctg acggcgtcag ggtcaacaat gaacgtcttg
     2581 aatatctggg tgatgcaatt atagacagca tcctgtcaga ttatcttttc cacctgtacc
     2641 cggaagcagc cgaagggttc ctgacaaaaa cccgtgcacg catcgtcaac cgcgagacgc
     2701 tcaaccagct tggcctttcc atgggacttg acaggctgat agtctccaac ctggcaccct
     2761 cagactcgcc ccgcaatctc tacggcaacg ccgttgaagc actcgtgggt gcgctgttca
     2821 tcgacagggg ctatgaccgc acccgcaaat ttcttatcga gcgcggactg aaaagacatt
     2881 tcaatctcag cgccttgctg gcagcggaga cggatttcaa gagtatgatc ctcgaatatt
     2941 gccagaagaa aaaacaaaag cttaacttca cctcacggga gaagcaatca ggcggcaacg
     3001 gtcaccccat cttcaccgtg acactcgaga tcagcaataa ggtgatttcg cagggcgaag
     3061 ggtcaacgaa gaaggatgct gagcaggttg cgtcgatggc tgccctcaat aaactgggtc
     3121 tgttaaaagg atgagcaggg aaaagaagat caccaggcat actatcggaa caggcgaacc
     3181 acagccattc ttttttctgg gagtggtgtc agctgagccc gattaccgtc tttcagtgat
     3241 gataaacaga catctgggaa ctgacctgag aaaatgcaat gaagatataa ccgttgccgg
     3301 tccggcagga acacagcagt tctcacgttt ctcacctgaa aaccgtgcat tttcactcgt
     3361 atcaaaccga agcggggtaa atgtcctgct gcgcaaactg aaaaacatcg attacctgct
     3421 ggtgcctggc ggcgttcagg ggaaaaaaga agctgaagag cttgccgctt cactgaggct
     3481 tatccccgaa ataaccgcag tgttcatttt tgacagccgc cacaacagtg accagaacct
     3541 ctcactcctg gcgctgtaaa agagaaagag gagacaattt ctcgcctcct cttcagtgtt
     3601 aaccctaaaa ctaacctaac ctatgaaaaa actccactta tactatcaca attatcgtgc
     3661 cataactgat gcaaatatca gaaccttttt ccggatattt tcttaaggat atgttaatga
     3721 attgatcttt aacatttttt taatactttg ttttttttac cttgcctcta tacctgttat
     3781 agaagattaa gtgtatctat taaagattta acagattaat tttgttaacc atgaaacgtt
     3841 catacattgt tctcctggcc atattctttt tcctggccat atctgccctg gtaataatcc
     3901 aggtcagatg gatcaacaat gtagttagcg cagaggatca gcaattcagg tttggagtga
     3961 atgaagccct taaagaggtg gtatcagagc tggagaaggc tgaaacatac aagcgcataa
     4021 tgagcgatat caaccccgaa ccgactccac aggagaccga tgaaacttca gaccagtcag
     4081 agcagaaatc agccgaagaa aagcttcttg aaaaatacgg tttcgacccc ggtgtcaggt
     4141 cagtaattat cagcagggcc ggttatacat acctgctcga ttccgacagt ctggaagttg
     4201 aatcattctt tgaaagtgct gagcctgacg ggcagattct cacagccggc gcaaccagca
     4261 ggatgaccag caagatcatc tccatagaaa aaattgtaag caaaatactc catgagactc
     4321 ctcccctgcg tgaccgtttc acggcagatg agctgaacaa cctgataagg agatcacttg
     4381 acgccgtggg tatccatctt gaatatgagt gttcggtgcg gggcgcctat ggcgatttaa
     4441 tttatataac accaaacttt cagggaagca gcggggcaaa caagtacctc cgccagctct
     4501 tcccgaatga cccggtgccc gggaacaata tccttacgat ctattttccc gaggaggaag
     4561 agtataagtt cagccagatc gcattcatgg caagctcatc aatgctgata gtcctgctgc
     4621 tgacactgct tgctacagga accttcatcg tcatcttccg ccagaagagg ctgtcagaga
     4681 tacggagcga cttcatcaac aacatgactc atgagcttaa aacgccgata gctaccatat
     4741 cactggcatc gcagatgctt gacgacccct ccatccctga tagctcgcga aatattggca
     4801 gcatagccac ggtgatcagg gaagagagca acaggctgaa ggtgctggtt gagagggtgc
     4861 tgcatacggc aatctttgaa aaagccaaac ttaagctcaa caaacagcct gttgacattc
     4921 attcactcat cttacggtca gttgatgcct tcaagctcca ggtaaacgga cgcggcggaa
     4981 ccatctcaac cttccttgac gcctctgacc cggttgtggc aattgatgag gccaacatct
     5041 tcaatgtgat gctcaacctc attgacaatg ccctcaagta caccctcgac acacctgaaa
     5101 taaccatcac aactgcaaca tggaacaggg gcatcatcat taccgtatca gacaacggaa
     5161 ttggcatccc gcgggagcac ctgaaacatg tcttcgaaaa gttttaccgc gttcctgcgg
     5221 gaaatacaca taatatcaaa ggatttggcc ttggactcag ctatgtgaag aagatcattg
     5281 aagagcatta cggcaccatc aaagtggaga gccagaccgc aaaaggaacc aggattatca
     5341 ttcatctttt aaaagaaccg ccaaaatgaa gaaaatcagc atattactgg cagaagatga
     5401 tcccaacctc gggcagttgc tgaagaacta cctcattgca agggagtacg atacaacact
     5461 ggtcaccgac ggtgcacagg ccatgcagat atacaggaaa gagagatatc agctttgcat
     5521 ccttgatgtt atgatgccgg aactcgacgg attcacgctt gcgaagaaaa tcagggcgct
     5581 tgaccctcac atcccgatca tcttccttac cgccaagaat cttaaggaag atgtgattga
     5641 aggattcaag tcaggtgctg atgactacct gaccaaacca ttttcaatgg aagagctgct
     5701 gtaccgcatt gaggccatac tgcgtcgtgc ctcaggaaaa atggccgaac ctcctgcatc
     5761 atcatacacc atcgggaaat tcacctttga cgtgacgaaa cagttgctga ttttcaatga
     5821 tcagtcgcgc aagctcacca ccaaggagtc tgagctcctg gagctgctct gccgccaccg
     5881 taatgaggtg cttgaacgta atttcgcact caagtccatc tggattgatg acaactattt
     5941 caatgcgcgg agtatggatg tttacatcac caaactgcgc aagtatctga gcagggatga
     6001 atccgtggag atactgaata tccacggcaa agggtacaaa ctgctttgct gagcaggatt
     6061 attccgaaac cgggcagggg cgcctggtgc aggactatac agaaaatggg caggggcgcc
     6121 aggtgcagga ctataccggg agcggccagg gataccgtgt acagggcaat tgcgaaaccg
     6181 gacagcgctg ccggatgcag gacactattc cgaaagcggg cccgactgac aggtgcacaa
     6241 atgaataccc cgcccggata acaatccgca cggggtatga gtctaggtta gtttcagggt
     6301 ctttcaacaa ataaaaaaat cttccctctc aaaacccaac gaagtgctaa gatagtatta
     6361 tttcttgatt tgcaatacct cccctctcat tttttatgtc agtcgaactt aagcactgcc
     6421 aggaatgcct gctgcggcac ctccacgttg cctatctggc gcattctttt cttgcccttc
     6481 ttctgtttct caagcagttt tcgcttcctg gtgatatcac cgccgtagca ctttgcggtt
     6541 acatccttac ggactgcctt gactgtttcg cgggcaatga tcttggcgcc tatggctgcc
     6601 tgtatggcaa tgtcaaactg ctgtctcggt atcagctcgc gcagcttttc acacatcctg
     6661 cgtccgaaat catatgcatg gcccctgtag ataagggttg agagtgcatc aaccatctcc
     6721 ccgttgagaa gtatgtcaag cctcaccaga tcagctttct tgtaaccggt gatatgatag
     6781 tcaaatgagg cataaccctt cgagatgctc ttcagtttgt catagaagtc gaacacaatc
     6841 tctgacagtg gcatgtcaaa ggttacctcc accctgtcac tggtgaggta aacctggttc
     6901 ttcatcgtac ctctcttgtc aatgcatagc ttcaggattg ctcccaggta ttccgacttc
     6961 gatatcacct gggcatgaat gtatggttcc tctatctcgt cgatggttgt tgcgggcggc
     7021 attcctgacg ggttgtgcac gtcaatgatc tcaccctttg ttgtcagcac acggaaagag
     7081 acattcggca cggtggttat gacatccatg tcgaactcgc ggtcgagccg ttcctgtatt
     7141 atctccatat gcagcagccc gaggaagccg cacctgaagc cgaagcccag ggcagcggat
     7201 gactcaggaa cgaaggtcag tgacgcatca ttgagttgca acttttcaat tgatgcccta
     7261 aggtcttcgt agtcatccgc atcaacgggg taaatgcctg caaagaccat aggcttcacg
     7321 tcggcgaagc cggagatggc cttgtcacac ggcctggcca catgtgttat tgtgtcgccc
     7381 acttttacct cggcagagac ctttatgcct gaaatgatat agcccacgtc acctgccctg
     7441 agcacatcgc gcggttcgcg cttaagcttc aggacaccta tctcatcggc atcatactcg
     7501 ctgccggtgt ttacaaactt caccatttca tgggtcctga tggtgccgtt gataattttg
     7561 aaataggcta tgatccccct gtatgagttg aagacagagt caaatatgag cgcctgaagg
     7621 ggtgcatccg ggtcgccttt gggggcaggt atcctggtga tgatctctga catgattcgg
     7681 tcaatgccca ttccggtgcg ggcgctggcc tcgattatct cttcccggct gcagccgatg
     7741 aggtcaacaa tctgatcctt gacctcttca ggcatggcgc tgggcgaatc catcttgttt
     7801 atcacaggga tgatctcaag cccgtgctca atggccatgt agaggttgga tattgtctga
     7861 gcctgaattc cctgggtggc gtcaaccacc aggagggctc cttcgcaggc agcgatggaa
     7921 cgcgaaacct cataggaaaa atcgacatgc ccgggggtgt caatcaggtt gagagaatac
     7981 tccgtcccct catactgata agacatctgt atggcgtggc tcttgatggt gattccacgc
     8041 tcacgttcaa gatccatatt atcaagaacc tgatcgtgaa aatcacgctc atcaacggta
     8101 ctggtcttct caagaagcct gtctgcaaga gtgctcttgc catggtctat gtgcgctatt
     8161 atgcaaaagt tgcgtatata ttccatcagc cgccgttcgg ttcaatacag gtgcaaatat
     8221 atacaatttg accctttctt aattaacccc cggattatga taccttaccg cattttctgc
     8281 caatttatca gattacctga tcaggaacag atgataaccc tgtcagtgtc ggtcacgttg
     8341 tttatcccgg gataccttgt ctgcggaaga gtggccgaaa gattcttcgg gatggactgg
     8401 cgctgccgat gaaggtttca ctgacagcag gcagagctgc tgcagtggca gcgttcgcac
     8461 tgtttctgac agtgctgagg aaaaagcggt tgcagtagaa tcagcaggtt tcaggcggag
     8521 gggcaatctc cgggtttttt cgcccggcta cgggcatatc acattcaagc ttcgcgatct
     8581 cgcggcggaa taagatgaat gatatcaccg ccgccacaaa atccgagacg ggaatggcta
     8641 cccatacacc tgatgcgccc cagaactccg gaagaatgat gatcagcgga aaaagaatga
     8701 ttatttgcct gagcatggag aggaatattg ccagcctggc ctttcctatc gcctggaaat
     8761 agttggaggc tatgatctgg aatccgacaa cgggcagggc ggcgcagtat atgcgaagcc
     8821 ctagtgtgcc tgcctcgcgc agttcagggc tgtctgtatt gaagatgctt attattaccc
     8881 cgggagcaag catacatatc agccatccgc caacagcaat cagcgtggcg tatctgattg
     8941 ccttcattat ggtctccttc acacggcaga agaggccggc gccatagttg aatccgataa
     9001 taggctgcac agccatattg atggagataa tcgagatcac cagcagcata gtggcgctgt
     9061 tgactatacc aagcgctgct acggagatgt caccgccgta cctgatgaat tttgtattca
     9121 ttactcccca gataatgctc gaggcgaggt tcatcgcaaa cggggcgaag cctatggaga
     9181 ttatatgcct gatgatatag aagtcaggtc tgaatgtggc agcggtgagc ctggtaacgc
     9241 tcctcttgct gcggaaatga agcaataccc atacggccag tacgagatgc gatatcaccg
     9301 tggcgatggc tgctcccttt actcccatgt caagtgcaaa gatgaatatc ggatcaagta
     9361 ttatgttcag gcctgcacta atgaatatgg aatacatcgc tatcctcgga ttaccctcgg
     9421 ccctgatgac gttgttcagt gagaagccca cggtggccaa tggtgttccg aggaggataa
     9481 tggtaaaata gtcagaggcg tatttgattg tctccggccc ggcgccaaag atctgcaaaa
     9541 cccggtcacg caccaggaac cctgctccca tcagtaccag tcccagaaca agtgaaagaa
     9601 agaatgcatt tcccaggatg cggttagccc tgttgaagtc cttttcacca agggcgagtg
     9661 agatacgtac tgccgagccc ataccaacga gcatcccgaa ggccatgaag actatcatca
     9721 gcgggaacac cactgtcaga cctgacagtg ccagtgcgtc aactccctgt ccgatataga
     9781 tgcggtcaac cacgttgtag agcgcgtttg ccatcatgct ggcaaatgcg gggaggaagt
     9841 atttccacat aagtctcccc ggtttctcgt gctcgagctc gtatacctgc ctcggatcag
     9901 tcatgactgc aaagatgggg aaatcaggca ataaaaagct gccccggagt ccggagcagc
     9961 ctttccaact aaatcaataa gctattttct tttacatcat accaggcatt ccgccgccgc
    10021 ccattgcggg gttcggcatc ggattctcct ctttctcctc gacaataact gcctcggtag
    10081 tgaggaacat acctgctatt gatgcggcat tttccagagc tatcctcgca acctttgccg
    10141 gatcaatcac accagccttc ataaggttct cgaacttgtc agtatgagca ttatacccaa
    10201 aatcgtcttt accttcgcgg actttctgca caataactgc accctcgagt ccggcattgg
    10261 taactatctg acgaagaggt tcttcgatag ccctctttac aatatcaata ccggtattct
    10321 gatcatcatt gtcgcctttc agtttttcaa gcaggggaat tgccctgatg taagccactc
    10381 cgcctccggg gataataccc tcgtctatag cagcacgtgt agcgtggaga gcatcgtcaa
    10441 cgcggtcctt cttttccttc atttccactt ctgaagcagc tcctatgtag agaactgcca
    10501 cgccgccggc cagtttggca agcctctcct ggagtttctc cttgtcgtag tctgaggtag
    10561 tggtggatat ctgctgcttg atctggttga tccgtgcctg gatcatttcc ttttcacctg
    10621 caccgttcac tatagtggta ttctccttat tgacggtcac tttgtcagcg acgccgagat
    10681 cagccatagt ggtattttcg agcttcatgc ctctctcttc cgagatgacg gtaccgcctg
    10741 tcagaatggc tatatcttca agcatctctt ttcttctgtc gccaaaaccc ggggctttga
    10801 cggcacaaac cttcagtgaa cctctcagac ggttcaccac cagggttgca agagcctcgc
    10861 cctcaacatc ctcggcaatg atcatcagac cacggccact gcgtgcggtt tcttcaagta
    10921 tcgggagaag gtctttcatt gatgagatct tcttgtcatg gatcaggatg tatggatttt
    10981 ccatctcaac ttccattttg tcaggatttg tgacaaaata ggctgaaatg tatccgcggt
    11041 cgaactgcat accttcaaca acctcaactt ttgtctcggt accttttgcc tcttccacgg
    11101 tgatgacgcc ttccttcttc accttgtcca tcgcagtggc gatgagttta cctatctcat
    11161 cgtcgttatt tgctgaaaca cgggcaacct gttcaatttt tttcagatcc tcgccaacgc
    11221 tctgtgactg gtttttaatg ctctcaacaa ctgttgccac agccttgtca attcctctct
    11281 tgaggtccat cgggttagca ccggcagtta cgtttttgag tcctacactg ataatgctct
    11341 gtgccagaac tgtggctgtg gtggtaccgt cacctgcagt gtctgaggtc tttgatgcaa
    11401 cttccttgac catctgtgca cccatattct ggtatggatc ggagagttcg atctcctttg
    11461 caacggtcac accgtccttg gtaatctgtg gagcgccgaa tttcttttcc aataccacgt
    11521 tgcgtccctt ggggccaaga gttaccttaa cggcatccgc aagacggtca acaccttcct
    11581 tcagaaggcc acgagcttcg atattaaatt ttatttcctt tgacattgtt attctttttt
    11641 aagttgttac gaaatataga gaacatcaga ctgagaaaga aggagatagt ctgtaccatc
    11701 aatattcagt tcctgaccgg aatatttccc atagagaaca acatctccct ttttaagttc
    11761 catctcttca tcttttttcg cagcaccaac aagaacaaca gtgcctgcgc ggggtttttc
    11821 ctttgctgag tcagggataa taatgccact ggcggtcttt tcctcagcct cctgaggctt
    11881 aacgaggacc ttacctgcca gcactttacc ttttaattgt gccattgcta gaagttttat
    11941 agttaaacaa tatttttcct gcttcagaat ttcataatct gtgccaaatg caagttggca
    12001 aaaaaaaagt gtcagttcat gacaaactga cactcctttg tctttatggt gtcactatta
    12061 ttcgttaccg ccgtcaggct gcccctgtac aggtgtctgt gtgggaagca ccggtatggc
    12121 gttgggatcc tgtgctcttt caattgacga cttgatgact gactctttcg caccctgtcc
    12181 tctcggaatt gaggcagtgg ctgccaggca aagaaccagg attgcaactc cgagggtcca
    12241 tgttgatttc tcaaggaagt cagctgtctt gcgtacgccc atcgactggc cggctgaggt
    12301 gaagtttgct gcgagaccgc ctcccttgga gttctgaacc agcaccacca gtattacaag
    12361 tgcacatgca ataataacca ggatcgtaag gaaaatataa atacccattg ttatactatt
    12421 ttattaaatc tttgatcttt tcaatacggc ttgcaaagta aacacttttt tccggatatt
    12481 gtattgagag tttttcataa atatttattg ccctggaata atagccctgg ttgatgtaaa
    12541 tttttgccag tgtttcagtt atgaatttgc catgttcctc cgtgctttct gccgacaggt
    12601 cctttaccgg ctggagatcg tccggagaga gcctctcaat tgaaggactg gttctgataa
    12661 agcggtcaat cagatcagcc ggggtcagct gggctgctgg ttctgcctct tccggcggcc
    12721 cgtccggggt cagttgcgct gctggttctg cctcttccgg cggctcatca ggggtcagtt
    12781 gtgtggcaga ctcagtctct acgaccggtt cgtccggaac cggctctgca gcaggctcat
    12841 actccagcac tagttcatcc ggaaccggct gtgcagcagg ctcatactcc agcaccggct
    12901 cgtccgggat cagctcgagg a
//
LOCUS       DAOPBK010000066        12915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4816_length_12915_cov_5.841757, whole genome shotgun sequence.
ACCESSION   DAOPBK010000066 DAOPBK010000000
VERSION     DAOPBK010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4816_length_12915_cov_5.841757"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1855)
                     /locus_tag="PLA17_05415"
     CDS             complement(287..1855)
                     /locus_tag="PLA17_05415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPK84886.1"
                     /translation="MKSSDELILSIDFGTQSVRSVLIDLEGNVLQLAKVEIEPYFSVA
                     PGWAEQYPEYFRDKMFESLRKLFSETTYSKHLIKAVTLTSQRSTLINLDSEGKSLRPA
                     ITWLDQRLSAVGKYPGKIMDLALRLIGMREAVHFAVKNGECNWIIQNQPEIWEKTGKY
                     LLLSGYLTWALTGEYRDSVGCTVAYLPFDYKTQKWTSRKHMNFKMFPVDREKLPDLVK
                     PSEVLGTISREASELSGIPAGLPVIAAAADKASEVLGSGVIKPDVACLSYGTTATVQT
                     THSRYHEVIPFFPSYPSAVPDCFNTEVQIYRGYWMISWFKNEFGLKEIQMAEQTGGYP
                     EIFLDDMVDDVPAGSMGLMLQPYWSPGVKVPGTEAKGAIIGFGDVHTRAHVYRAILEG
                     LTYSLKDGLNRTVKRTKIPAKSVIVSGGGSQSRQAMQITADIFNLETFRPHTYETSAL
                     GAAINAAVGMKYYSDFPAAVAAMTRKGDRFVPDPHNVEIYSELYEKVYLKMYRHLQPL
                     YEQIRDVTGYPPKI"
     gene            complement(1865..3493)
                     /locus_tag="PLA17_05420"
     CDS             complement(1865..3493)
                     /locus_tag="PLA17_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPK84887.1"
                     /translation="MENGNSYRHILKWGDKHEESISHHMAEVIKEKFGLPEADFKRKH
                     LPGTEPVKIDKPSMLKPAQLDFFRSVSGAENIMTDDLSRARFSCGKFYGELLDLRFGL
                     VPDPPDGVVAPRTHEEVEKIISYCSDEGIAVVPAGGLSSVTGAVRAPAGGIALDLTKH
                     LNRVLAVNKVNKTVTVQAGIYGPALEEELNRQGYSCGHFPQSFEYSTVGGWVSARGAG
                     QASTGYGKIEDMLVSVRAVTPAGVIATGDFPRTAQGWDIFRLFAGAEGTLGVITEATL
                     HIFNYAPENTASAGFIFRSFEAAVEAMRTIIQAECGKPHLFRISDPDETDIAFRTSGF
                     DGTLADRVLQMLGFKTGSRCLMFVTVEGEKEYTRFVARKIKRKARAGKGMYIGTTPVR
                     KWLEQRYSSAYLRDPMMDLGIMTDTLETAVTWENLLNVWKAAHDYVARRAKAFMMIHI
                     SHVYENGANLYFTFLSPMEKGNERHDFTTFHRGLVETILKHGGSISHHHGVGRVLAPW
                     MEGHLGRSSMELLRAVKKHLDPNNIMNPGGTLGL"
     gene            complement(3498..5132)
                     /locus_tag="PLA17_05425"
     CDS             complement(3498..5132)
                     /locus_tag="PLA17_05425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPK84888.1"
                     /translation="MMLKRYIEEVGPNKFDLVIIGGGITGAAVAYIAAARGLSVALFE
                     KKDYGGATSAATSKLIHGGLRYLANGELKLVRESLRERRILGNIAPNFVYPLPFLLPM
                     YKRWRGNIWKMLIGMYLYDLLSYDKKDTWDKSKKLENHRLINRRKTIRVEPNLLKENL
                     RNAFYFFDYQSIFPERLTLAFIKSAAEYGASVSNYTPVEGFIYGEGRKITGVRVRDLF
                     TDRTREVYANLVVNCGGTWADKILSLASGEGEVKHRVKRSEGIHIITRKIAGNHVVSL
                     QKPDGGHMMIMPWREHSLIGTTDKEFFGDPDDYKVSMESIDEIIENVNTNYGRKIARE
                     DIIHAYGGLRPLVDDQTKGSYETSRKYEVYDNAADGIEGMITVEGGKYTTSRSLAREV
                     LRLISLKLNRTLSDPVSDNLYLSGCEIRDMRQFMIRQHLNYKDFSRNTVEYVSRNYGT
                     ESQVVFRIARSNTRFAEKVTHDGEILAEVVYAIQYESARTLRDIMLRRTGIGTLGNPG
                     KAMIDKIASLAAGMMGWSEKRKEDEIASLMKVYDIF"
     gene            complement(5349..6650)
                     /gene="creD"
                     /locus_tag="PLA17_05430"
     CDS             complement(5349..6650)
                     /gene="creD"
                     /locus_tag="PLA17_05430"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HPK84889.1"
                     /translation="METENLKQEKWYESMTFKMVLIGIMGLFLLIPLALIKDVIRERS
                     ANAETARTEIGQTWASAQTVTGPVLNIPGTQVIDKDGNFITTTMHILPDELRVNGVLE
                     PEIRYRGIYETVVYESEMEISGSFTIAGFEQLNDYTWEWDKAYISLGVSDNKGISNKA
                     ELSIGGRVISAIPGAGQSDLFDRGISFPYAIDPSKLAESGGEFTLELHLRGSSSIAFS
                     PVGQTTKVALSSKWNAPKFTGNFLPAERNVTNEGFTASWVVTHLNRSFPQVWKGKHYS
                     PADDAFGVDLILEADHYTKAERSAKYGLLFIALTFLVLIIIELRFSRRIHIFYYLLVA
                     LALIMFFSLLTALSEHIGFNPAYLISAVATIGLLTFFFRSLLDKGWMTLLISGLLTGL
                     YVFIFFLLAMKDYAFLAGNIGLFVLLAALMIISARYRLFRS"
     gene            complement(6705..7001)
                     /locus_tag="PLA17_05435"
     CDS             complement(6705..7001)
                     /locus_tag="PLA17_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPK84890.1"
                     /translation="MKQILANFNKVFESRVRLGIMSLLAVNPGMDFTGLKETLAVTDG
                     NLASHLRALEEAGYIRMKKSFINRRPNTRYSITDEGMESFSSHLKALEELIRNQ"
     gene            complement(6998..7636)
                     /locus_tag="PLA17_05440"
     CDS             complement(6998..7636)
                     /locus_tag="PLA17_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84891.1"
                     /translation="MTNQTDQHLEDIQAIRRLMESSSRFLSLSGLSGIITGVLGIAGA
                     LGAQLIITRICAPVDWYARPFAEGPDGIREFLPLFVMMAVILILAFSVAAFLSTRKAR
                     KSGIKAWTPVTRRMLVSLLIPLGTGGLFILLTAATVPAGVIVASTLIFYGLALISAGK
                     FTFGEIHWLGVLEVAAGLFCLALPQYSVLIWVLGFGLLHIVYGLFMHLRYRG"
     gene            complement(7720..>8772)
                     /locus_tag="PLA17_05445"
     CDS             complement(7720..>8772)
                     /locus_tag="PLA17_05445"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HPK84892.1"
                     /translation="PVTPGAAKSTGGIVALVALMLVVLMLAGCETVIDYKGPETEPKA
                     VIYALLEPDRFISVTISESHSVFQIPWKPRQIRDATVRVYRDGKLLETLAYQGSDPAD
                     ENAPVSPYSKYTSETNKPEYGHVYRIEVMIPGYPAAYGETELPAPVAVELADTTYSDI
                     GYGNRRLTVKLRFRDPADGKNYYRLTAPSTRGNYYGDHGQPYDPEQKVYVRINDLSYG
                     ALGDPIISPKQEDDEFFDVYPQNEYYVFTDDLIPGKEYTLNLEYNGFHFSTEHYEFLR
                     AFFRLNSITRDLYLYLQSYSAYNRTADNPFAEPVPVYSNITNGLGIVGALSFSQDSLI
                     IGEYPVEGVQYEMQLY"
     assembly_gap    8773..8872
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8876..11335)
                     /locus_tag="PLA17_05450"
     CDS             complement(8876..11335)
                     /locus_tag="PLA17_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK84893.1"
                     /translation="MRILLFLVFTALALSLSLEAQRSITGSSAATARITVSGFVTDAG
                     SGERMIDATVYERASFAGTTTNNYGFYSLPLPAGKAELIVSYLGYRPDTLVVQLSRDT
                     VINFSLVMLSSEIAAVTVTASGRRAKIESTQMSMIDIPVEKFLKLPVIFGEADVLKVI
                     QLLPGVQSGTEGSTGIYVRGGGPDQNLFLLDGVPVYNANHLFGFMSVFNPDAVKSVQL
                     YKGGFPARFGERLSSVVDIRMKEGNEKEFHGNVSVGLISSKLSLEGPIIKDKTSFVIS
                     GRRTYYDILTRPFIMKFNENQNDKTNAGYYFYDLNAKINHKFSEKSRLYLSSYLGRDR
                     AYMRHTSRSSYQSEDGEKRFEYKDDFGLGWGNFITALRWNYLITNKLFSNTTLTFSKY
                     DFDVTIESSVEDLNSKAKGVDYFKYFSGIEDVGAKTDFDYYLSPNHSVKFGAGYTYHH
                     FKPGITTYRFNSGIIGETGLDTVVGDMKVRAGEFIMYAEDNIDLTPNLKMNAGVRLSL
                     FNVQDTSYFVFQPRLSLRYIATNDLSFKLSYSKMGQFVHLLTTSAVSLPTDLWLPVTK
                     RYEPPVSHQVALGSGFLIPGDLEVSVEAFYKTMDNLIEYKEGASFGGTGEGWESKVEK
                     GRGWAYGSEIMIEKNYGKLTGWIGYTLSWTRRKFENLNYGNTFPAKYDRRHDVSLALT
                     YKFNDKVDAGLVWVYGTGNAATLGASQYPGDNLFVHDSRYTMITDFPGRNNYRLPSYH
                     RLDLGVNLHRQRKNGMSTWSFSVYNAYNRLNPFFVYWGEEWVEEPDPDNPGEKIYYSK
                     PELRKVTVFPIIPSVSYSFKF"
     gene            complement(11341..11751)
                     /locus_tag="PLA17_05455"
     CDS             complement(11341..11751)
                     /locus_tag="PLA17_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84894.1"
                     /translation="MKQLVLRNSLYARIYRSLGIVCLIMVIIIIITGDKNEWSFWIQI
                     LALLLLGLAFLSLNFGTLVNRLTVEKGVLTIKWYTKPMRIILPIHEIDEIMTDENFVR
                     IVMKTGRIIRLPTRLLEFEEKQAVRKFLKETTGF"
     gene            complement(11901..12311)
                     /locus_tag="PLA17_05460"
     CDS             complement(11901..12311)
                     /locus_tag="PLA17_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84895.1"
                     /translation="MNQLVLRDKVQEYLFRFLGVLTLLSVGLQLFIQSPKTAIFWMLA
                     VVFILVAIFFLTLNFGALINRITADRGILTIRWNTKILKKRLPIDQIAEITEDERYIR
                     ITMKDGSNIRLHVKHMDADKRRAARKFLKENTGL"
BASE COUNT         3118 a         3320 c         3109 g         3268 t
ORIGIN      
        1 gccttatgtt gaatgtcatc acatctattg ccggatcgtc agccggtaca catgaaaaga
       61 aaagaattgc aaaaagagca gtggtgataa gtgatctgaa catatcttat gaagtttgtt
      121 tgaagacagt ctaaagttac tttaaaaatt agttatcccg aaggaatgaa ccattgcgga
      181 gatcaatgaa cagatcttca gcggtaaagt ggcagcgctg acggaatccg gggctgatga
      241 agtgataatt gaaaatggcg gcaacatctt catgaaactg acagcatcag atcttcggcg
      301 ggtagccggt cacatccctg atctgctcat aaagaggctg cagatgacgg tacatcttca
      361 gatacacctt ctcatacaac tcactgtaaa tctccacatt gtgcgggtca ggcacaaatc
      421 tgtcaccctt cctggtcatt gccgccaccg ctgccgggaa atcactgtaa tacttcattc
      481 ctacagccgc gttaattgcc gccccgagag ccgaggtctc ataggtgtgc ggcctgaagg
      541 tctcaagatt gaatatatca gccgtgatct gcatcgcctg cctgctctga gaacctcccc
      601 cggaaactat aacacttttt gcaggtattt tcgtgcgttt aaccgtccgg ttaagtccat
      661 ctttaagtga ataggtgagc ccttcaagta tggcacggta cacatgcgca cgggtatgaa
      721 catcaccaaa ccctataatc gcacctttgg cttcagttcc cggaactttt acgccgggcg
      781 accagtaggg ctggagcatc aggcccattg agcctgccgg cacatcgtca accatgtcat
      841 caaggaagat ctcagggtaa ccgccagtct gctcggccat ctgtatctcc ttcaggccaa
      901 actcattctt gaaccagctg atcatccagt agcccctgta tatctgcacc tcggtgttaa
      961 agcagtcagg gacggcgctg ggatatgacg ggaagaacgg tatcacctcg tgatatctgc
     1021 tgtgggtggt ctgcaccgtt gccgtggtgc cataactgag gcatgccaca tcaggcttga
     1081 tgactccgga gcccagtacc tcactggcct tgtcagccgc agcagctatc accggcagcc
     1141 cggcaggaat gcctgacagc tccgaagcct cgcgactgat tgtccccaac acctcagagg
     1201 gcttaaccag gtctggcagt ttctccctgt caacaggaaa catcttgaag ttcatatgct
     1261 tcctgctggt ccacttctgg gtcttgtaat caaacggcag ataagcaacc gtacacccta
     1321 ccgaatcgcg gtactctcct gtcagggccc atgtcagata gccggagaga aggagatact
     1381 ttccggtctt ctcccatatc tccggttgat tctgaataat ccagttgcac tcgccgttct
     1441 tgacggcaaa atgaacagcc tccctcatgc caatgagcct cagggccaga tccataatct
     1501 ttccgggata cttccccact gcactaagcc gctggtcaag ccatgtaatg gccgggcgga
     1561 gcgacttccc ctcagaatcg aggtttatca gcgtcgatcg ctgcgaggtg agtgtcactg
     1621 cctttatgag atgcttgctg taagtggtct cgctgaagag cttgcgaagc gactcgaaca
     1681 tcttgtccct gaagtactcg gggtactgtt cggcccatcc gggagccacg gagaaataag
     1741 gctcaatctc aacctttgcc agctgaagca catttccttc aaggtcaata agcacggaac
     1801 gtaccgactg ggtgccgaag tcgatagaca atatcagttc atcagatgat ttcatgtcag
     1861 gttgttaaag gcccagcgta ccgccgggat tcattatgtt attcgggtca aggtgtttct
     1921 tcactgccct gagcagttcc atggagctcc tgccaaggtg cccttccatc cacggcgcca
     1981 gaaccctgcc cacaccatga tgatgcgaga ttgagccacc gtgtttcagg atggtctcta
     2041 ccagtccgcg gtgaaaagtg gtaaaatcat gtctctcatt tcctttttcc ataggactca
     2101 ggaaggtgaa gtagaggttg gcgccgttct cataaacatg tgatatgtga atcatcatga
     2161 aagcctttgc ccggcgtgca acataatcat gcgccgcctt ccagacattc agcaggttct
     2221 cccaggtaac cgcagtctcg agcgtgtcgg tcatgatgcc caggtccatc atcgggtcac
     2281 gcagataggc gctggagtat ctctgttcca gccacttcct gaccggagtg gttcctatgt
     2341 acatcccctt ccctgccctg gctttccttt ttatctttct ggcaacgaac cttgtatact
     2401 ccttctctcc ctcaaccgtc acgaacatca ggcagcgact gccggttttg aatcccagca
     2461 tctgcagcac cctgtcggca agggtgccat cgaacccgct tgtccggaat gcaatatctg
     2521 tctcgtcagg atcagagatc cggaacaggt gtggcttgcc gcattcagcc tggataatgg
     2581 tgcgcatagc ctcaacggct gcctcaaatg atctgaatat aaaaccggcg gaggcagtgt
     2641 tttcaggagc ataattgaag atatggagcg tggcttcggt gataacgccc agtgttcctt
     2701 ccgcaccggc gaagagcctg aagatatccc agccctgtgc agtacgcggg aaatcacctg
     2761 ttgctatcac tccggcgggt gtcacagccc tgaccgacac cagcatgtct tcaatcttgc
     2821 cgtaccccgt cgatgcctgt cctgcccccc tggcggagac ccagcctccc acggttgaat
     2881 actcaaaaga ttgcgggaag tgaccgcagc tgtaaccctg cctgttgagc tcctcttcga
     2941 gtgccggccc gtatatgccg gcctgcacgg taacggtctt atttacttta ttgacagcca
     3001 gcaccctgtt gagatgcttc gtgaggtcaa gtgcgatgcc tcctgcaggt gcgcgtacgg
     3061 cacccgtcac ggatgatagc ccgcctgcgg gaacaacggc tatcccctcg tcactgcagt
     3121 aactgattat tttttccacc tcttcatgag tacggggagc caccactcca tcaggagggt
     3181 caggcacaag gccgaagcgc agatcaagca gctcaccgta aaactttcca catgagaaac
     3241 gtgcccgcga cagatcatct gtcattatgt tctccgcacc cgaaacactc ctgaaaaaat
     3301 caagctgggc cggtttgagc atcgaaggct tatcaatctt cacaggttct gttcccggca
     3361 ggtgctttct tttaaaatcc gcttccggca ggccaaattt ttcctttatc acttcagcca
     3421 tgtgatggct tatgctctcc tcatgcttgt ctccccactt cagaatgtga cggtaactgt
     3481 tcccgttctc cataatgtca gaatatatcg taaaccttca tgagtgatgc aatctcatcc
     3541 tcttttctct tttcgctcca acccatcatt ccggccgcca gcgaggcaat tttatcaatc
     3601 atcgctttgc cgggattgcc cagtgtgccg ataccggtcc tccgcagcat gatatcacgg
     3661 agtgtcctgg cgctctcgta ttgtatcgcg tagaccacct cggcaagtat ctccccgtcg
     3721 tgagtgacct tctcggcaaa cctggtgtta ctgcgggcaa tgcggaagac aacctggctc
     3781 tcggtaccgt agttcctgct cacatactca acggtgttac ggctgaaatc cttgtaattg
     3841 aggtgctgcc tgatcatgaa ctgcctcatg tcacgtatct cacaacccga caggtataga
     3901 ttgtcggaga ccggatcaga cagggtgcgg ttgagcttca gcgagatcag cctcagcacc
     3961 tcgcgtgcaa ggctgcggct ggtggtgtac ttgccaccct ccaccgtgat catcccctca
     4021 atgccgtcag cagcattgtc atagacctca tatttcctgg aggtctcgta ggagcccttc
     4081 gtctggtcat ctaccagcgg acgaagaccg ccataggcat gaataatatc ttcacgcgca
     4141 atcttccggc cgtaattggt gtttacattt tcaatgatct catcaatgct ctccatcgaa
     4201 accttatagt cgtcagggtc accaaaaaat tccttgtcgg ttgtacctat cagcgagtgt
     4261 tcgcgccagg gcatgatcat catatgacct ccgtcaggct tctgcagtga gaccacatgg
     4321 ttgcccgcga tcttccttgt gatgatatgt atcccttccg accgtttgac acggtgttta
     4381 acctctcctt cgccggaggc aaggctcagt atcttgtcag cccaggtgcc tccgcagttg
     4441 actaccaggt tggcatatac ctccctggtc cggtcggtga aaaggtccct caccctgact
     4501 ccggtgatct tcctcccctc tccataaatg aatccttcca caggcgtgta gttggagacc
     4561 gaagcaccgt attcggcagc cgacttgatg aaggccagtg tcaggcgttc agggaaaata
     4621 ctctgataat caaagaagta aaaggcattc cgcaggtttt ccttcagcag gttgggctct
     4681 accctgatgg ttttccttcg gtttataagg cggtggttct ccagtttctt gctcttgtcc
     4741 catgtatcct ttttatcgta tgagaggagg tcatagaggt acattcctat gagcatcttc
     4801 cagatattgc ctcgccaacg cttgtacatc ggaaggagga aagggagcgg gtagacgaag
     4861 ttcggcgcta tgttgccgag aatccgtcgt tcgcggagcg actcacgtac cagcttcagc
     4921 tcgccgttgg cgagatagcg gagtccgcca tggatgagtt tcgaggtggc tgccgaggtg
     4981 gcgcctccat agtccttttt ctcgaagaga gcaaccgaga ggccccgcgc agcagcgata
     5041 tatgcaacgg cagcaccggt gatgcctccg ccgataataa ccaggtcgaa cttattaggc
     5101 cccacttctt caatatacct tttaagcatc attgatcagt cagtttaaac gatgctgaaa
     5161 gttagtcgaa aaagctgaga atcctcatga ggcaaatcaa atcattccct tattaaaagc
     5221 tctccccgat gcgtgacgtc ttgcatcgga gaaagaggcc ggggataagg gaggagggaa
     5281 acccaaccgt cctccctttg cagtgagaat tcgcgggcgg gtgccggagg cattgcgccc
     5341 tgcataaact acgacctgaa gagcctgtac ctggcagata tgatcatcag cgctgccaga
     5401 agcacgaaga ggccaatgtt gccggcaagg aacgcataat ccttcattgc aagcaggaag
     5461 aaaatgaaca cgtaaagtcc tgtcagcagg ccactgatga gcagggtcat ccagccctta
     5521 tccagcaacg acctgaaaaa gaaggtcaaa agaccgatgg tggccaccgc ggagatgagg
     5581 taggcaggat tgaagccgat atgctcgctc aaggcggtaa gcagtgagaa aaacatgata
     5641 agcgccagtg ccacaagcag ataatagaag atatgtattc tccgggaaaa tcgcagttca
     5701 atgattatca gtaccaggaa ggtgagagcg atgaaaagga gaccgtattt ggcgctgcgc
     5761 tctgccttgg tgtaatggtc tgcctccaga atcaggtcaa cgccaaaggc atcatcagcc
     5821 ggggaataat gtttgccctt ccacacctgc gggaagctcc tgttaagatg ggtcacaacc
     5881 cagctggccg tgaacccttc gttagtcaca ttccgttccg caggaaggaa attaccggta
     5941 aatttgggtg cgttccattt tgaagagaga gcaacctttg tagtctgccc cacaggagag
     6001 aaggctatgc tgctgcttcc gcggagatgc aactcaagag tgaactcccc tcctgattct
     6061 gccagttttg aaggatcaat ggcatagggg aaagagatac ccctgtcaaa cagatcactc
     6121 tgccccgcac cgggaatggc gctgatgacc cttcccccga ttgaaagctc agccttgtta
     6181 cttatccctt tattgtcgga gacccccaat gatatgtagg ccttgtccca ctcccaggtg
     6241 taatcattga gttgctcaaa tcctgcaatg gtaaaactgc cggagatttc catctctgat
     6301 tcatatacaa cagtctcata aatccccctg taccttatct caggttccag cacaccgttg
     6361 accctgagtt catcgggaag gatatgcatg gttgtagtta tgaagttgcc gtctttatct
     6421 ataacctgtg ttcccggaat attcagcacc ggaccggtta ccgtctgggc cgacgcccag
     6481 gtctggccaa tctcggtacg ggcagtctcg gcattcgccg aacgctccct tatcacatcc
     6541 tttatcaacg caagcggaat gagcaaaaac agacccatta ttccgatcag taccatcttg
     6601 aaggtcatcg actcatacca tttttcctgt tttaagtttt ctgtttccat ttttattgag
     6661 ctgattaaag tactttgtat ttcaaagtat aggtataaaa aaatttattg gtttctgata
     6721 agttcctcga gcgccttcag atggctgctg aagctctcca tcccctcatc tgtgattgag
     6781 taacgggtgt tgggcctgcg gtttatgaag ctcttcttca tcctgatata accggcctcc
     6841 tccagagccc gcaaatgact ggccaggttg ccgtcagtga ctgcaagtgt ctccttcagg
     6901 ccggtgaagt ccatgccggg attcaccgcc agcagtgaca tgatacccag cctgacgcgg
     6961 ctctcaaaga ccttgttgaa gtttgcaagt atctgtttca tcccctgtac ctgagatgca
     7021 tgaatagtcc gtagacaatg tgcaggagtc caaatcccag aacccatatc aacacgctgt
     7081 actgcggcag tgcgaggcag aagagacctg cagccacctc aagcactccc agccagtgta
     7141 tctcgccgaa agtgaatttc ccggcactga taagggcaag tccatagaag atcagggttg
     7201 acgctactat gacaccggca ggaacagttg cggctgtcag aagaatgaac aatccgcctg
     7261 tgcccaatgg gatgagaagg ctcaccagca tcctcctggt gacgggtgtc catgctttga
     7321 tgccactttt ccgggctttc cgggttgaaa ggaatgctgc aacggaaaac gccagtatca
     7381 gaatcacagc catcatcaca aacaggggaa ggaactcacg gattccgtcc ggaccctctg
     7441 caaatggccg tgcataccag tcaacaggag cgcaaatcct ggtgatgatg agctgtgccc
     7501 ccaatgctcc ggcaatgcca agaactcctg taataatacc ggacaagccg ctgagcgaaa
     7561 gaaacctgga ggaagactcc attagcctcc tgatggcctg gatatcttca agatgctgat
     7621 ctgtctgatt tgtcattgta tttcgtagta ctttgcacta caaagtaaaa taatgctttt
     7681 tattcctgca agaaaaagtg agattttttt gggggaaagt caataaagct gcatctcata
     7741 ctgcaccccc tcaaccgggt actctcctat tatcagggaa tcctgtgaaa atgaaagtgc
     7801 tcccacaatc cccagtccgt tggtaatgtt ggaatatacc ggcacgggtt cagcaaacgg
     7861 attatccgct gtccggttat aagccgaata tgactgaagg tacagataaa ggtccctggt
     7921 tattgagttc aacctgaaga atgcgcggag gaactcgtaa tgctcagtac tgaaatgaaa
     7981 tccgttgtac tcaagattga gagtgtactc tttgccgggg ataagatcat cagtgaagac
     8041 ataatactca ttctgagggt agacatcaaa gaattcatca tcctcctgct ttggtgaaat
     8101 gatcggatcg ccgagggcac cgtagctgag gtcattgatg cggacataaa ccttttgttc
     8161 aggatcataa ggctggccgt ggtcaccata gtagttcccc cttgttgacg gtgcagttaa
     8221 cctgtagtag tttttcccgt cagccggatc gcggaagcgc agtttcacgg tcagccgcct
     8281 gttgccgtaa ccgatatcac tgtatgtggt gtcagccaac tcaacagcca ccggggctgg
     8341 cagctctgtc tctccgtaag ccgccgggta tcccggaatc ataacttcaa tacggtagac
     8401 atgcccgtat tccggtttat ttgtttcaga ggtgtacttc gagtagggac tcacaggagc
     8461 gttctcatca gccgggtcgg aaccctggta tgcaagggtt tccaaaagct taccgtcacg
     8521 gtaaacccgt actgtagcat ccctgatctg ccttggcttc catggtatct ggaagacaga
     8581 atggctctcc gatatcgtta ccgaaataaa cctgtccggt tcaagaaggg catagatgac
     8641 tgcctttggc tcggtctcag ggcccttata gtcaatcacc gtttcgcaac cggcaagcat
     8701 cagcactaca agcataaggg caaccagagc gactattcca cccgtgctct tcgcggcacc
     8761 aggggtgact ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccatcaga
     8881 acttgaatga atatgatacg gacggtatta tcggaaagac agtcaccttt ctcagttcag
     8941 gcttgctgta atagatcttt tctcccggat tatccggatc gggttcttcc acccattctt
     9001 cgccccagta gacaaagaac gggttgagcc ggttataggc attgtacacc gagaagctcc
     9061 atgtgctcat tccatttttt ctctgtctgt gaaggtttac ccccagatcg agtctgtggt
     9121 aagatggtaa acgatagttg ttgcgcccgg gaaaatcagt aatcatagta taccgtgaat
     9181 catgtacaaa gagattatca ccagggtact gtgaagctcc cagcgtggca gcgttccctg
     9241 ttccgtatac ccataccagt ccggcatcaa ccttgtcatt gaacttatat gtcagcgcga
     9301 ggctcacgtc atgccttcgg tcatacttcg ccgggaaagt gttcccatag ttcagattct
     9361 cgaatttgcg ccgggtccat gaaagagtat atcctatcca tcccgtcagc tttccgtagt
     9421 tcttttcaat cattatctct gatccgtagg cccatcccct gcccttctcc accttcgact
     9481 cccatccttc acccgtacct ccgaaggagg ctccctcctt gtactcaata aggttatcca
     9541 tggtcttgta gaatgcttcc accgatacct ccaggtctcc gggtatcagg aatcctgatc
     9601 ccagtgccac ctgatgcgag acaggtggct cataccgctt tgtaaccggc agccagaggt
     9661 ccgtcggaag gcttacggca gaagtggtaa gcaggtgtac gaactgcccc atcttactat
     9721 atgacagctt gaacgacagg tcattggtgg ctatgtaccg gagcgagagc cgcggctgaa
     9781 agacgaaata gcttgtgtcc tgaacgttga agagtgacag gcgaacaccg gcattcatct
     9841 tcagattcgg ggtgaggtcg atgttgtcct cggcatacat aatgaactca ccggctctaa
     9901 ccttcatgtc tcccacaacg gtatcaagcc cggtttcgcc aattataccc gaattaaacc
     9961 tgtaagtggt gatgccgggc ttgaagtgat ggtatgtgta accggcgccg aacttcacgg
    10021 aatggtttgg cgacaggtag tagtcgaagt ctgtctttgc accaacatcc tcaatcccgg
    10081 agaaatactt gaagtaatcc actccctttg cttttgaatt gagatcctca acagacgatt
    10141 caattgtcac atcgaaatca tatttgctga aggtaagggt agtattactg aacaacttat
    10201 tggtaatcag ataattccat ctgagagcag tgatgaagtt accccaaccc agtccgaaat
    10261 catctttgta ttcaaacctt ttttctccgt cctcactctg gtaacttgac ctggaggtgt
    10321 gtctcatgta tgccctgtct cgtccaaggt acgagctcag atagagtctg ctcttctccg
    10381 aaaacttgtg gttgatcttg gcattgagat cgtagaaata ataacctgcg tttgtcttgt
    10441 cattctgatt ctcgttgaat ttcatgataa acggcctggt aaggatatca taatatgtac
    10501 gtcgaccgga gatgacaaat gaagtcttgt cttttattat cggcccctca agtgacagtt
    10561 tggatgatat cagccctacg gagacatttc catggaactc cttctcgttg ccctctttca
    10621 tgcgtatgtc aaccactgat gacaggcgtt caccgaaccg ggccgggaat ccgcctttgt
    10681 aaagctggac tgatttgacg gcatcgggat tgaataccga catgaatccg aagaggtggt
    10741 tggcattgta gacaggtact ccatcgagga gaaagaggtt ctggtcaggg ccgcctccac
    10801 gtacatagat gccggtggat ccttcggtgc ccgactgcac acccgggagc agctgtatca
    10861 ctttcagcac atcggcttcg ccgaagatca ccggcaactt gagaaatttc tccaccggga
    10921 tgtctatcat gctcatctgg gtgctttcga tcttcgccct ccggccggag gccgtaactg
    10981 taacggcagc tatctcgctt gataacataa ccaggctgaa gttgataacc gtgtcacggg
    11041 atagttgtac gacaagggtg tcaggcctgt agcccaggta tgatacgatc agttcagcct
    11101 tcccggcagg cagtggcagg ctgtagaatc catagttgtt agtggtggta ccggcgaatg
    11161 aggctctttc ataaacggtg gcgtcgatca ttcgttcgcc gctgccagca tcggtgacga
    11221 agccgctgac ggtgatcctt gcagttgcgg cagatgagcc ggttatactc cgctgcgcct
    11281 caagagacaa gctcagggcg agtgctgtaa aaacgaggaa gagcagtatg cgcatacatt
    11341 tcagaagccg gttgtctctt tcaggaattt ccggacggcc tgtttctcct cgaactcaag
    11401 caacctcgtc gggagccgga tgatacgacc tgttttcatc acgatacgga caaagttttc
    11461 gtcggtcata atctcatcga tctcatgtat cggcaggatt atcctcatgg gtttagtgta
    11521 ccattttatt gtcagcacac ctttttcgac tgtcagcctg ttaacgagtg ttccgaagtt
    11581 caggctgagg aatgccagcc ccagcaggag cagggctaaa atctggatcc agaaagacca
    11641 ttcattttta tcaccggtaa tgattattat gataaccatg atcaggcaga cgatccccag
    11701 cgagcggtat atccgtgcat agagtgagtt cctgagtaca agctgtttca tgacagtaag
    11761 gtttattatt gtaaagatag gaaatcctat ggactggatg atggagtatt atgattttcc
    11821 tgaagtcaaa gaaatcatcc ggtggcaggg tgagttgccg gccggcaggg tgcggttcag
    11881 agtaaacaat gcagcggatt ctacagtccc gtgttctcct tcaggaattt gcgtgctgcc
    11941 cggcgtttgt cggcatccat gtgtttcacg tgaaggcgga tgttactgcc gtctttcatg
    12001 gttatccgga tgtatctctc atcctccgtg atctcagcga tctggtcaat ggggagacgt
    12061 ttttttaaga tttttgtatt ccatctgatt gtcagtattc cgcggtcagc ggtaatccgg
    12121 tttataaggg caccgaagtt cagggtcaga aagaagatgg caaccaggat aaaaaccaca
    12181 gccagcatcc agaagatggc ggttttggga ctttgaatga agagttgtaa cccgacactg
    12241 agcagtgtca gtactcccag aaaccggaag agatattcct gaaccttgtc tctaagcacc
    12301 agttgattca tcaggactga attggtttca acaataaagt taaataaaaa cggtgactaa
    12361 tggcaagata accgtaatga ttaagcacct gcggtcttca acttttggat ccgaaaacaa
    12421 ttttgtaaag aaagccatga ttatcatgac cattaacacc agagtgagtc tggagtttta
    12481 ttttcatttt agctgagttg ttcgggaact acggattttt tcgtctgagt tgaagaatgc
    12541 acgacattcc aatcagtaat gccacaatgc aggtccctgt cattgccact ctgctgtatg
    12601 ggccggaagg tggcattatg agtgctgtca atgaaagaat aaggcaaatg atgctcagga
    12661 ttaatcctgc ccacaatatc ttccttcttc tgctggtgtt catgtcagat tattattttt
    12721 tgttagtgtt ccaatcaatg tgtctcagat ggcagatctt catcgaagtt ttacatctca
    12781 aatagtcgac ccttaacaag tcatgttaaa gatagccatt tgcgaacact cctatacaga
    12841 tattgtaaag agaagccagc aaattatcca gtgagtgaag tataaacacc aggattatac
    12901 cgccctcttt tcggt
//
LOCUS       DAOPBK010000067        12895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4825_length_12895_cov_5.510826, whole genome shotgun sequence.
ACCESSION   DAOPBK010000067 DAOPBK010000000
VERSION     DAOPBK010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12895
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4825_length_12895_cov_5.510826"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLA17_05465"
     CDS             <1..194
                     /locus_tag="PLA17_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK84896.1"
                     /translation="RFLIGKIDVLELNNADTKKDQNKRNYIQALNNYWTYFFNIRALT
                     LYDFINRKPLETDYEKLVE"
     gene            341..3376
                     /gene="secDF"
                     /locus_tag="PLA17_05470"
     CDS             341..3376
                     /gene="secDF"
                     /locus_tag="PLA17_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPK84897.1"
                     /translation="MQNKGLISALAIALSIVCIYQLSFTVASLKVKKEARVYAAGDLN
                     KEVAYIDSVANLPKDQWKFLGNSYKEVQTKEINLGLDLKGGMNVVLEISVEDIIRALS
                     NYSTDKTFNEAIALAKQKQLGGGKEDFITLFVRSYEELDPAARLAGIFGTVELKGRVN
                     FNSTNAEVQKVLEGEINSAIDNAFNVLRNRIDRFGVIQPNISQLQPRGRILVELPGVK
                     DPERVRDLLQGTANLEFWETYDNPEVIGYLMQANDFLRSIMPTEVIAAATDTVAAEAV
                     TEAVTDAVQDTAATGEKSLLEMVSSDTAAAAEASSLEEFTRQNPLFGLLRPNVSLEGQ
                     PEPGSIVGFAAGKDTARVNSYLAMNQIKSMFPRELKFYWSQNPHKWDPTNTLYELHAI
                     RITTRDGRAPLTGDVVTSARPSTGVTGSDIKVDFSMNAEGAKIWQRMTRDNIGRCIAV
                     VLDDYVRSYPRVQNEIAGGNTEITGDFTLEEAEDLANILKSGKMPAPARIIQDTVVGP
                     SLGKEAINSGMKSFIIAFFVVLLYMIFYYSRSAGLVADIAMTANLFFMMGVLVSLNAV
                     LTLPGIAGIVLSIGMSVDANVLIFERIKEELRGGKGVKQAIADGYKGASSAIIDSNLT
                     TLLTGVILYYFGTGPIKGFASTLVIGIITSLFSAVLVSRLIYEFLLKRNVSLKFSASY
                     TENFLQNMHIDFVGIRKYGYIFSITITILAIITLSTKGLSKGIDFTGGRTYVVRFEQP
                     VNTVEIANLLSDEFEGDPMVVTFGSENQVKISTKFKVEDPAATDEVNGLLYDGLKELI
                     PGVDQQTFISDYIVSSETVGPTIAKDQASKAVWAILLALVGMFFYIFFRFRRWQFGLG
                     SLVSLAHDVIVVLGLYSILQGVMPFSMDIDQSFIAAILTVVGYSINDTVVIFDRIREY
                     LPIYRKRTKREVYNMALNSTLARTLNTGMATIFVLIVIFIFGGEVIRGFVFALLFGIV
                     FGTYSSLFVSTPVLYETTRGKDDDEAVPVEAEKKKKIAG"
     assembly_gap    3783..3882
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3946..4275
                     /locus_tag="PLA17_05475"
     CDS             3946..4275
                     /locus_tag="PLA17_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPK84898.1"
                     /translation="MGLLLFISGQEIVLILIAVLLLFGADKLPGIARAMGKGMREVRK
                     ATDEIRKEFDETTGDFRKDINDITDSVRRDITDVTDSVRKDFEDAAAPLSDEINQAAR
                     DINKELG"
     gene            4272..4925
                     /locus_tag="PLA17_05480"
     CDS             4272..4925
                     /locus_tag="PLA17_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK84899.1"
                     /translation="MIKATGIVKSYGDLRVLKGIDLEVAEREVVAIVGASGAGKTTLL
                     QIIGTLDKADSGTILINGQNPGQLSQKALAAFRNKNIGFVFQFHQLLPEFTALENVCI
                     PALIAGKSRTEAMERASELLDFLGMSDRLDHKPSQLSGGEQQRVAVARAIVNSPSMIL
                     ADEPSGNLDTENKTELHKLFFRLRDTFGHTFIIVTHDPQLASMSDRTIRIKDGLITG"
     gene            5522..6097
                     /locus_tag="PLA17_05485"
     CDS             5522..6097
                     /locus_tag="PLA17_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HPK84900.1"
                     /translation="MEKQSTHSISADKTDTKQLNTLKRENSKEESNLNMQISRELQEY
                     FKNTEFENLVSPGQSDLTLSGFLNNKLLIVFTIRTGIPYSLFALIKDYTPFTFNDWAE
                     YLNISGKSLTRYRQSDKFFKPIHTEKIIELAEVTNLGLGVFGSENKFKMWLETPNFAL
                     GNQKPFELLKDSYGKEMVMGELTRIDQGILA"
     gene            6097..6555
                     /locus_tag="PLA17_05490"
     CDS             6097..6555
                     /locus_tag="PLA17_05490"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HPK84901.1"
                     /translation="MEVFRLSKKKWASELSGKGAALKGARWNSPGTEIIYTASNRSLA
                     MAEVAVHLTLGTLPDDYMMVTIVIPDDVSLQSIRESDLPRFWNSFPYLPVTQTVGDNF
                     IRENSHCLLKIPSAVTKGDFNILINPFHPDFSRIRIKSIEPFPFDKRIFK"
     gene            6826..7596
                     /locus_tag="PLA17_05495"
     CDS             6826..7596
                     /locus_tag="PLA17_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPK84902.1"
                     /translation="MVQNIKDLKVFLDEKVAQYNNPTFIELDPIQIPHSYTTKEDIEI
                     SGFLSAIISWGNRTSIIKNAKRMMGYMGDSPYDFILNHNYRHLKQMDGFVHRTFNSDD
                     LLTFIEGLKHLYNGKNGLEGIFVKYQTNDSLQPAIHELKKEFFNIPHLGRTRKHLPDP
                     MTGSAAKKINMFLRWMVRRDDRGVDFGLWKSISPSLLSCPLDVHSGNVARRLGLLTRK
                     QSDAKAVAELDARLREFDPADPVKYDFALFGLGVFEHF"
     gene            7733..8446
                     /locus_tag="PLA17_05500"
     CDS             7733..8446
                     /locus_tag="PLA17_05500"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPK84903.1"
                     /translation="MANKYFRFKKFTVRQEQASFKVTTDSVLLGAWAQIEDAGTILDI
                     GTGTGLLALMAAQRSSARIVAIEPDNPSFRQALLNFECSPWKKRIMLLNVSLQEFKPE
                     TGRGFDAIIVNPPYFIDSLLNPDNNRARTRHTVTLSHSELLEAVVRLLNPGGSLHLVL
                     PVTGAEKFITLAESYGFFCNRRMMVRPTPEKAPVRVLMTFGREAIPCNEEELVIEKGG
                     RHIYSDEYVSLTKEFYLKF"
     gene            complement(8766..10103)
                     /locus_tag="PLA17_05505"
     CDS             complement(8766..10103)
                     /locus_tag="PLA17_05505"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPK84904.1"
                     /translation="MMKKVLILGAGLVSKPMVEYLLNEGFQVIVATRTVEKAQKLIGG
                     HKNGKAIAWVMEDKETLSDMVRDCDLAVSLLPYRFHVDVAKLCLKHKKHLVTTSYISP
                     AMQALHDDAVKTGVIFLNEVGLDPGIDHMSAMRVIDHVKQKGGHIEEFYSLCGALPAP
                     EDANNPFGYKFSWSPKGVVLASRNGATYLKHGKIVTIEPIDLFKAHFLHTFPGVGEME
                     VYPNRDSVSFVDIYGLKGVQTMYRGTYRFKGWCESLDLMKAIGLVNDGDKDYTGVTFR
                     QFVAEMAGVPGKDIKKELKEKPGLNASDKALEALEWLGLFDNTDMGYGVTSPFEITSD
                     LMIKKMWMNDNERDMVVMQHLFLVTYNDGKSEVISSRMLDFGTPATNTSIARTVALPA
                     AMAVKMILTGKINLHGVYRPTLPELYNPILDELEANGIKMVEEYGLPVDQMIK"
     gene            10376..>12895
                     /gene="mutS"
                     /locus_tag="PLA17_05510"
     CDS             10376..>12895
                     /gene="mutS"
                     /locus_tag="PLA17_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPK84905.1"
                     /translation="MQKYVETPLMKQYYSVKAKHPDAILLFRVGDFYETFGEDAIRAS
                     EILGITLTRRANGAASSVELAGFPFHALDTYLPRLVRAGQRVAICEQLEDPKLAKKIV
                     KRGITELITPGVSFSDNVLNHKENNFLAAIHIEKQSVGVALLDVSTGEFFVSEGDTPH
                     VEQILNNFQPREVLFEKRKTELFHERFGSRFYTYRLDDWIFTPDTANDRLLKQFGTTS
                     LKGFGVQGMTSGIIAAGAILWYLDMTRHENLGHINRLARIEEEKYVWLDRFTVRNLEL
                     FHSPFEGAKTLIEIIDRTLSPMGGRMMKRWLSLPLKDISVLNTRLDIVEYLIGHPDVK
                     EMIDGLIREVGDLERLLSKVAVRRVTPREVAQIWRALKAIGELKSICAATEFQPLVFL
                     ASGLDPCPELAETISRTLAPDPPTAVSKGHVIAEGISQELDELRKIYYGGKDYLNDLQ
                     QRESIRTGISSLKISYNNVFGYYIEVRNTHKDKVPPDWIRKQTLVSAERYITEELKEY
                     EEKILGAGEKILALETSLFNDLVVSIEPYINLMQADAAAVGRLDCLQSFAVVSDEYGY
                     VRPVLDDSHVLDIREGRHPVIERQLPPDDPYVPNDVWLDNDEQQVIILTGPNMSGKSA
                     LLRQTALIVIMAQTGCFVPAKEARIGIIDKIFTRVGASDNLSLGESTFMVEMNEAASI
                     LNNLSDRSLVLLDEIGRGTSTYDGISIAWAMVEYIHECRHRAKTLFATHYHELNEMEN
                     TFSRVKNFNVSIRELDDRVIFLRKLKRGGSEHSFGIHVARMAGMPKSVVGRANEILKM
                     LEEGRGGTNLGKPVEEIATHREGYQLSFYQLDDPVLKQIRDE"
BASE COUNT         3381 a         3000 c         3119 g         3295 t
ORIGIN      
        1 agcggttcct gattggcaag atagacgttc ttgaactgaa caacgcagat acgaagaaag
       61 accagaacaa gcgcaattat atccaggcac ttaataatta ctggacctat tttttcaata
      121 tcagggctct taccctgtat gatttcatta accgtaagcc gctggagacc gactacgaaa
      181 agctggtcga ataaattgaa aaggataatg tgaaactgcc ggtttatgcc ggcagttttg
      241 cttattaatc ataatggttt aagaaaataa agtcttatat ttgcgcctcg taaatattta
      301 agtgtttcac ttacaatcat aatattaaaa gcggatacag atgcagaaca aaggattaat
      361 ttcagctctg gctattgccc tgtccattgt ttgcatttac cagctttcct tcacggtggc
      421 ttcattgaag gtcaagaagg aggcaagggt atatgcagca ggtgacctga acaaggaggt
      481 ggcatacatt gactcggttg ccaacctgcc gaaggatcag tggaaattcc tgggcaattc
      541 ctataaggag gtgcaaacca aggagatcaa cctgggtctt gacctcaagg gcggaatgaa
      601 cgtggtgctt gagatttcag tagaggacat tatcagggct ctttcaaact atagtacaga
      661 caagacattc aatgaagcaa ttgcattggc gaagcagaaa cagcttggcg gaggcaagga
      721 agattttata acgctgtttg tacgctccta tgaagagctt gatccggctg ccagactcgc
      781 cggaatcttt ggaaccgttg agctgaaagg ccgcgtcaat ttcaattcaa ccaatgctga
      841 agtacagaag gtgctggagg gagagatcaa ctcagccata gacaacgcat tcaacgtgct
      901 caggaacagg attgaccgtt ttggcgttat acagcccaac atctcgcagc ttcagccgcg
      961 tggccgtatc ctggttgaat tgccgggagt gaaagacccg gaaagggtac gcgaccttct
     1021 gcaggggaca gctaatctcg agttctggga aacgtatgat aaccccgagg taatcggtta
     1081 cctgatgcag gctaatgact ttctcaggtc aataatgcca actgaagtca ttgctgcggc
     1141 tactgacact gttgctgcgg aagctgtcac ggaagctgtc acggatgctg tgcaggacac
     1201 agccgccacc ggcgagaaat cacttcttga aatggtcagc agtgacaccg ctgctgctgc
     1261 cgaggcatcc tccctcgagg agttcacccg ccagaatccc ctcttcggat tgctgagacc
     1321 aaacgtatca cttgagggac agcccgaacc gggcagtata gtgggcttcg ccgcaggcaa
     1381 ggacaccgca agggtcaata gctacctggc gatgaaccag atcaaatcaa tgtttcccag
     1441 ggagcttaag ttctactgga gccagaatcc gcataaatgg gacccgacaa atactcttta
     1501 cgagcttcat gccatcagga tcaccacacg tgacggccgt gctcctctga ctggtgacgt
     1561 tgtcacttca gcacggcctt caacaggcgt cacgggctct gatatcaagg ttgatttctc
     1621 aatgaatgcc gagggtgcga agatatggca gcgcatgaca cgtgacaaca taggcaggtg
     1681 cattgcagtg gtacttgatg actacgtaag gtcatacccg cgcgttcaga atgagattgc
     1741 cggaggaaac accgagatca cgggcgactt caccctcgag gaggccgaag accttgcaaa
     1801 tatcctcaag tcagggaaga tgcctgcacc tgcacgcatc atccaggata cggttgtagg
     1861 cccttcgctt ggaaaggagg caatcaacag cggcatgaaa tctttcatta tcgccttctt
     1921 tgtcgtgctc ttgtacatga tcttctacta cagccgctca gcaggtctgg tggcagacat
     1981 tgccatgact gccaacctct tcttcatgat gggcgtcctt gtctcgctca atgcggttct
     2041 gaccctgccg ggcatagcag gtattgtgct ctcaataggt atgtcggttg acgcaaacgt
     2101 gctcatcttc gagagaatca aggaggaact caggggaggc aagggggtta agcaggccat
     2161 tgctgacggt tacaagggag cctcttctgc catcatcgac agtaacctta caacactgct
     2221 taccggtgtg attctatatt acttcggtac aggtcctatc aaaggttttg catctacact
     2281 ggttatcggt atcattacct cactcttctc ggcagtgctt gtctcacgcc tgatctatga
     2341 attcctgctc aagcgtaatg taagcctgaa attctctgct tcatataccg agaatttcct
     2401 gcagaatatg catatcgatt tcgttggtat aaggaaatac ggttatatat tttccattac
     2461 aattacaatc cttgcaatta taaccctttc aaccaagggg ctcagcaaag gtattgactt
     2521 caccggaggc cgtacctatg tggtaaggtt tgagcagcct gtcaataccg tcgagattgc
     2581 taatctcctg tcagatgaat ttgaaggcga tcctatggtt gtgaccttcg gttcagaaaa
     2641 ccaggtgaag atcagcacca agttcaaggt tgaagacccg gcagccactg acgaggtcaa
     2701 tggtttgctg tacgatggtc tgaaggagct tattcccgga gttgaccagc agacgtttat
     2761 cagcgattat attgtcagtt cagagaccgt agggccgaca atcgcaaagg accaggcctc
     2821 gaaggcagtc tgggcgatac tgcttgcact tgtgggcatg ttcttctata tattcttcag
     2881 gttccgcaga tggcagttcg gtctcggatc tttggtttca cttgcacatg acgtgatcgt
     2941 tgtgcttggt ctatattcga ttttgcaggg cgtcatgcca ttctccatgg atattgacca
     3001 gtcgtttatt gcagctatac ttacggtggt tggttactcg ataaacgaca ccgtggttat
     3061 cttcgacagg atcagggagt atctcccgat ttacaggaaa cggacaaaga gggaggtcta
     3121 caatatggct ctcaacagca ccctggcccg tacactcaac accggtatgg caaccatctt
     3181 tgttcttatt gtgatcttca tattcggagg ggaggtgatc agaggatttg tctttgccct
     3241 gctcttcggt attgtcttcg gaacctattc atcgctcttc gtttcaacac ctgtgctcta
     3301 tgaaacaacc agggggaaag atgatgatga ggctgttccc gtagaggcgg aaaagaaaaa
     3361 gaaaatagcc ggataacggc tcccgcactg aggctgcccc gaaaggcagc ctcagccact
     3421 tcttccgggg gctgcctcat tctttctttt cctggctgcc tcaattgttt ctctccggca
     3481 gcctcctcac atcttctctg gcagcctcct catttcaagt gtgcccggca gcctcgtccg
     3541 cctcttcctg tcagccacag tcactccttt cctgatgtct tactcactgc tttccggtag
     3601 ccttagttgc ttcttcccgg ccgcttgagt tgttccctcc ttacctcctc actcacttct
     3661 agtgtgccct gcagcttcag tcgtttctct ccgggcagtc tcagtcactt cttctctggc
     3721 agcctcctca tttcttccca tcagcttcat atttatttac taactttgcc tggcataaaa
     3781 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcatttct tcccatcagc
     3901 ttcatattta tttactaact ttgcctggca taaaatgcca ccccaatggg attattgcta
     3961 ttcataagcg ggcaggaaat agtattgata ctgattgcgg ttctgctgct tttcggagct
     4021 gataagctgc cgggaatagc acgtgccatg ggcaaaggga tgcgcgaggt tcgcaaagcc
     4081 actgacgaga tcaggaagga gttcgacgag actaccgggg atttcagaaa agatatcaat
     4141 gatattactg actcggtcag gcgtgacatt acagatgtga ctgacagcgt gaggaaggac
     4201 tttgaggatg cagccgcacc gctgtccgat gagataaatc aggcagcacg cgacataaat
     4261 aaagaactag gatgataaag gcaaccggaa tagtgaagtc gtacggtgac ctcagggtac
     4321 tcaagggcat tgatcttgaa gtggctgaaa gagaagtggt tgccattgtc ggtgccagcg
     4381 gggcaggcaa gacaaccctc ctccagatca ttggcacact tgacaaggct gattcaggga
     4441 cgatattgat taatggccag aaccccgggc agctttcaca gaaagccctg gctgccttca
     4501 ggaataaaaa catcggcttc gtctttcagt tccaccagtt gcttcctgaa ttcaccgcac
     4561 ttgaaaatgt gtgcattccg gcgctgattg cggggaaatc acgtaccgag gctatggaaa
     4621 gagcctcgga gcttctcgat ttcctgggaa tgagtgacag gcttgatcat aagccttcac
     4681 agctcagtgg tggtgagcag cagcgtgtgg cagtggcacg tgcaatagtg aacagcccct
     4741 caatgatact ggctgatgaa ccttcgggaa acctggatac agagaacaag accgaactgc
     4801 ataagctgtt ttttcggttg cgtgacacct tcggacatac attcatcatt gtaacccatg
     4861 atccgcaact ggcctcaatg tcagaccgga caataaggat aaaggacggt ctgattaccg
     4921 gttgaagctg atttcggagt cttgaaagat gaagaaattg ttttacggta tgatgaaaac
     4981 aaggaacttg aatatcgggc aaggagaatt gcatattgga acagtgagaa aaagtttgtt
     5041 cgagtttatt accatcaatt gtgaattgaa agctgagaaa gcaggctgaa aacgtaaatg
     5101 gtcatttccc ggcatagtga ccgtgaaacg gctctaattg atgaattaca tcaatatcta
     5161 aagctttttg gaagacccgg atggtagttg gagaaaggtt atacaagtaa cctatattaa
     5221 ctgccaaatt tactcttccc cgaaatgcgg gggcttactt ttaaaaatca aagatcgtaa
     5281 tgctgagaat cagtagtttg cgagaaaaaa ataatgactt gccggtttgc cggacaacaa
     5341 tgatctcatt tattaaaagt tcctgatccg gcaatctgcc agtcaatgac gcgcagtata
     5401 aaccgtgaaa aagaatccct tgttgcattc tgtaagggca gcgagcttca atgctatata
     5461 aatttggaaa ttaagacaat tggcttatct ttgtagacat atttccagac aaaaaaatat
     5521 tatggaaaag caatcaacgc atagcatcag tgctgacaaa acagatacga agcagttaaa
     5581 taccctcaag agagagaatt ctaaagagga gagtaatctg aatatgcaga ttagcaggga
     5641 gttacaagaa tactttaaga atacagaatt tgaaaatctg gtctcacccg gtcaatctga
     5701 tctgacatta tcggggttcc tgaataataa actcctcatc gtctttacaa tcaggacagg
     5761 tatcccctat tctttatttg cccttatcaa ggattatacc ccgtttacat tcaatgactg
     5821 ggcagaatat ctgaacattt cgggtaaatc acttacaaga tacagacagt cagataaatt
     5881 ctttaagcca atccatactg aaaagatcat tgaacttgct gaggtcacaa accttggtct
     5941 gggggttttt ggttcggaaa ataagtttaa aatgtggctt gaaacaccaa attttgctct
     6001 tggcaatcaa aaaccctttg agctactgaa agattcatac ggtaaagaga tggttatggg
     6061 agaacttaca cgcattgatc aggggattct tgcctaatgg aagttttcag gttatcgaaa
     6121 aagaagtggg cctccgaact atcgggtaag ggagctgcac ttaagggagc ccgctggaat
     6181 tcccccggta ctgaaattat ttatactgca tcaaacagat cactggcaat ggctgaagta
     6241 gccgttcatc tgacacttgg aactttacct gacgattata tgatggtaac gattgtaatt
     6301 cctgatgatg tttcactgca atcaatccgg gaaagtgatc tgccccgttt ctggaactca
     6361 tttccttatc tgccggtgac ccagacagtg ggtgataatt ttatcaggga gaacagtcat
     6421 tgtttattga aaataccatc cgccgttaca aaaggcgatt tcaatattct cattaatccc
     6481 ttccacccgg atttcagcag gatcaggatt aaaagtattg aacctttccc ctttgacaaa
     6541 aggattttta agtgaaaccc gcatgtatta aatgtataaa tacgtatgta tataattttc
     6601 aaatatgtgg attccatcag accttgcaaa tcaaaattag acactgatga aaaaaatgaa
     6661 tctccttcag caaaaaagtc caaaattatt ttcgccggat agcattatta tacctgaatt
     6721 atccttacta atcatcaatc tgaagtacat gttgagtcag gtaatcaagt agcgtaggaa
     6781 acccttatca taattcttac ttttaccatg tttaaagata tatgcatggt acaaaacata
     6841 aaagatttga aagtttttct tgatgaaaag gtagctcaat ataacaatcc aactttcatt
     6901 gaactcgatc cgattcaaat acctcattcg tacacaacaa aggaagacat cgaaatatcc
     6961 ggttttttat cagcgataat ctcatggggg aatcgcactt ccattataaa aaatgcaaag
     7021 cggatgatgg gatacatggg agattctcct tatgatttta tacttaatca taattaccgt
     7081 catctgaagc aaatggacgg ttttgtgcat cgtacattta attcagatga ccttttgaca
     7141 ttcattgaag gcctgaagca cctatataat ggtaaaaacg gactggaagg aatatttgtg
     7201 aaatatcaga ccaatgattc attgcaacct gcaattcatg aattaaagaa agaatttttt
     7261 aatatcccgc atcttgggag aacccgaaaa caccttcctg acccgatgac tggttctgct
     7321 gcaaaaaaaa taaacatgtt cctgaggtgg atggtgcgca gggatgaccg gggtgttgat
     7381 ttcggcctat ggaagagcat ctctccgtca ctgctctcct gcccgctgga tgttcattcc
     7441 ggaaatgtgg cccgcaggct gggcctgctt acacgcaagc agagcgacgc aaaggcggtg
     7501 gcagaacttg atgcccggct gcgcgagttt gatccggctg acccggttaa gtatgacttc
     7561 gcgcttttcg ggctgggggt gttcgagcat ttctgaacag agactctatt gcagcaccca
     7621 attcctttcc tttaagttgt tggggggggg tgttcgaaca tttctgaaca gagacttaaa
     7681 ctgattacca atcttccggc gacggccggc gacaaactga cgataatccg gtatggcaaa
     7741 caaatatttc aggttcaaga agtttaccgt gcgtcaggag caggcttctt tcaaggttac
     7801 aaccgacagc gtgctgcttg gcgcctgggc gcaaatagag gatgccggta caatactgga
     7861 tatcggtacg ggaacaggcc tgctggcgct gatggcagca cagaggtcct ccgcacggat
     7921 tgtggccatt gagccagaca acccctcttt ccggcaggca ttgcttaatt ttgaatgcag
     7981 cccgtggaaa aagagaataa tgcttttgaa tgtctctctc caggagttta agccggaaac
     8041 cggtcgcggt tttgatgcaa taattgtcaa tcctccctat ttcatcgatt cactactcaa
     8101 ccctgacaat aacagggccc gtacaagaca taccgtgacc ctcagccact ctgaacttct
     8161 tgaggctgtg gtgcgactgc tgaatccggg gggctcactt cacctggtat taccggtaac
     8221 cggggcggaa aagtttatca cccttgcgga gagttatggc ttcttttgca acaggcggat
     8281 gatggtcaga ccaacacctg aaaaggctcc ggtcagggtt ctgatgacct tcggccgcga
     8341 ggccataccc tgcaatgagg aggaactggt catagaaaaa ggagggagac acatctactc
     8401 tgacgagtat gtctccctca caaaagagtt ttatctgaaa ttttgatcgc tgacccgaat
     8461 ccgggttctg cgatattttc atctgcatct tgcccgggaa atcaaccatg actgcccggt
     8521 tcctggctct gcggttttcc ctgcgcagtc tgcccggcaa ctcacccctg acaatcccgg
     8581 ttcctgcttc tccgattttt tcgtctgcag gttacccggg agattaatcc cgtccagccg
     8641 gttcctggct ctgcggtttt ccctgcgcag tctgcccggc aactcacccc tgacaatccc
     8701 ggttcctgct tctccgattt tttcgtctgc aggttacccg ggaaatcacc actgacagcc
     8761 agaccctatt ttatcatctg atcaaccgga aggccgtact cctctaccat ctttatgccg
     8821 ttggcttcaa gctcatcgag gatgggattg tacagttcag gaagagttgg cctgtaaacg
     8881 ccgtgcagat tgatcttccc tgtaaggatc atcttgacag ccattgctgc cggcagagcc
     8941 acggtacgtg ctatggaggt attggttgcc ggggtgccga aatcaagcat cctcgagctg
     9001 ataacctcac tcttgccgtc attgtatgtc acgaggaaga ggtgttgcat gacaaccatg
     9061 tcacgctcat tgtcgttcat ccacatcttc tttatcataa ggtcagaggt gatctcaaat
     9121 ggtgaggtta ccccataacc catatcggta ttgtcaaaga gccccagcca ctccaatgct
     9181 tccagtgcct tgtcgctcgc gttgagtcct ggtttctcct tcagttcttt tttgatatcc
     9241 ttgcccggta caccggccat ttcagcaacg aactgcctga aggtgacgcc tgtgtaatcc
     9301 ttgtcgccgt cattgaccag tcctattgcc ttcatcaggt caagactctc gcaccatccc
     9361 ttgaagcggt atgtgccgcg gtacatggtc tgaacccctt tcagtccgta aatatccaca
     9421 aagcttacgg agtcgcggtt gggataaact tccatttcgc caactcccgg gaacgtatgg
     9481 aggaaatgtg ccttgaaaag gtcaataggc tctatagtca caatttttcc atgcttcaga
     9541 tatgttgcgc cgttacggct tgcaagcacc acacctttcg ggctccagga aaacttgtag
     9601 ccaaacgggt tatttgcatc ttccggagcc ggcagagcac cgcagagcga ataaaattcc
     9661 tctatatggc cacctttctg ctttacatgg tctattacac gcattgctga catgtggtca
     9721 ataccggggt caagtccgac ttcattaagg aagatgaccc ctgtcttcac ggcgtcatca
     9781 tgcagggcct gcatggcggg tgaaatatat gatgttgtga caagatgctt cttgtgtttg
     9841 agacataact tcgccacatc aacatgaaac ctgtaaggca gaagactgac agccaggtca
     9901 cagtccctta ccatgtcaga gagggtctcc ttgtcttcca ttacccacgc tattgccttg
     9961 ccattcttat gtccgccgat cagcttttga gccttctcaa cagtcctggt ggcaacgata
    10021 acctgaaaac cttcattaag caggtactcg accattggct ttgataccag gccggcaccg
    10081 agaataagta cttttttcat cataatataa ttttgatttt attttaatgg tatgatggaa
    10141 ctcgcatagc tattattata tacacaggta tttgtgtatt tgatacatgc ggatttcata
    10201 tacgttatgt tctctgatat ttcggatccc tgcaaggtca ttttcatgag cctccctgac
    10261 aaatataacc aaacatttgc ggactattta tgataaaagt tatgcttaag ttgcgggaga
    10321 tttttaattt tgtttcacgc tgttgcgcga gggtataaaa ctgtaaatat ctcagatgca
    10381 gaaatatgtc gaaacaccgc tgatgaagca gtattattcc gtcaaggcca aacaccctga
    10441 cgcgatactc ctgttcaggg tgggcgactt ctacgagact ttcggcgaag atgcgatacg
    10501 tgcatcggaa atactgggca taaccctgac caggagggcc aacggcgcag ccagctcggt
    10561 tgaacttgcc gggttcccgt ttcatgcgct tgacacatac cttcccagac ttgtcagagc
    10621 agggcagaga gtggcaatat gtgagcagct cgaagacccg aagcttgcaa aaaagattgt
    10681 caaacggggc atcactgaac tgatcacccc gggagtctca ttcagtgaca atgtgctgaa
    10741 tcacaaggag aataacttcc tggccgccat tcatatcgag aaacagtcgg ttggcgttgc
    10801 actactcgat gtctccaccg gcgagttctt tgtctccgag ggcgatacgc ctcatgtgga
    10861 acagattctc aataatttcc agcccagaga ggtgcttttc gagaaacgaa aaaccgagct
    10921 cttccacgaa cggttcggca gccggtttta cacatacaga ctcgacgact ggatatttac
    10981 cccggacaca gccaacgaca ggctgctgaa gcagttcgga accacatcac tgaagggctt
    11041 tggtgtgcag ggcatgacaa gcggtatcat cgccgcaggc gcaatcctct ggtaccttga
    11101 catgacccgt catgaaaatc ttggccacat caaccgcctc gcgcgtatcg aggaggagaa
    11161 atacgtatgg ctcgacaggt tcaccgtccg taatcttgaa ctgttccatt cgcccttcga
    11221 gggcgcaaag acgcttatag agatcattga ccggacgctt tcgcccatgg gcggaagaat
    11281 gatgaagcgg tggctctcac tcccgctgaa ggatatttcg gtgctgaata ccaggcttga
    11341 catcgtggag tacctgattg gccatcccga tgtgaaagag atgatcgatg ggctgatacg
    11401 cgaggtgggt gacctcgaaa ggctgctgag caaggtggcg gtgaggaggg tcactccacg
    11461 tgaggtggca cagatatggc gggcattgaa agctatcggc gaattgaaga gtatctgcgc
    11521 tgccacggag ttccagccgc tggtattcct ggcatcaggc ctggatccct gccccgaact
    11581 ggcagagacc atcagccgga cactggctcc cgatcctccg actgccgtca gcaaaggaca
    11641 cgttatcgcc gaaggtattt cgcaggagct ggatgaactc cgaaagatct attacggagg
    11701 aaaggattac ctgaacgacc tgcagcagag agagagtatc cggaccggta tcagctcgct
    11761 taaaatcagc tataacaacg tttttggcta ctatatcgaa gtgcgcaata cacataagga
    11821 caaggttccg cctgactgga tacgcaagca gaccctggtg agcgcagaaa gatatatcac
    11881 tgaggaactt aaggagtacg aagagaaaat ccttggtgca ggagaaaaga tactggcact
    11941 ggagacctcg ctgttcaatg acctggtggt ttcaatagaa ccatatataa acctgatgca
    12001 ggctgatgct gcggcggtag ggaggctcga ctgcctgcag tcatttgcgg tggtttctga
    12061 tgagtatggc tacgtgagac cggtgctgga cgacagccat gtgcttgata tcagggaggg
    12121 tcgtcatccg gtgattgagc gccagttgcc gcctgatgat ccgtatgtcc ccaatgatgt
    12181 gtggcttgac aatgatgagc agcaggtgat catactgacc ggtccgaata tgtcgggtaa
    12241 gtcggcgttg ctgaggcaaa cggccctgat tgtgattatg gcgcagaccg gctgtttcgt
    12301 tccggcaaag gaagcgagga taggcatcat tgataagata ttcaccagag tgggagcatc
    12361 ggataatctc tccctcgggg agtcaacctt tatggttgag atgaatgagg ctgccagcat
    12421 cctcaataat ctgtcggaca gaagcctcgt gctgcttgat gagatagggc ggggaacaag
    12481 cacctatgac ggcatttcca ttgcctgggc catggtagag tatatccacg aatgcaggca
    12541 cagagccaaa acgctctttg caactcatta ccatgaactc aacgagatgg aaaatacctt
    12601 cagcagagta aagaatttca atgtctcaat acgggagctt gacgacaggg tgattttcct
    12661 ccgcaagctg aagaggggag ggagtgagca tagtttcggg atacacgtag cgcgtatggc
    12721 cggcatgcca aagagtgttg tcggcagggc taatgagata ctgaagatgc ttgaagaggg
    12781 gcgtggcggc acgaatctgg gcaagccggt tgaggagatt gccacgcatc gcgaggggta
    12841 ccagctgagc ttctaccagc ttgatgaccc ggtgctcaaa cagatacggg atgaa
//
LOCUS       DAOPBK010000068        12837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4853_length_12837_cov_5.536770, whole genome shotgun sequence.
ACCESSION   DAOPBK010000068 DAOPBK010000000
VERSION     DAOPBK010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12837
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4853_length_12837_cov_5.536770"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLA17_05515"
     CDS             complement(<1..761)
                     /locus_tag="PLA17_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84906.1"
                     /translation="MRKRIVIFRNEAADDHLPWVEACNRRQGEVEAEVIDITASDWLE
                     RFHMKNYDLVLLRPPGRTELFKRLYDERAHILYNYAGVPVYPSLNEVLIYENKRFLSD
                     WLNINNIPYPQTRVFFDRKEAMEYAGSGVRLPLVAKTNIGASGNGVRFIHDKKELYDY
                     IHNAFGKGVGVRTGPKLRKGSIAGKLIRAFTIRGFVKKRMGEYRASYLNPQRGFVIFQ
                     EFVPHEFEWRCVRIGDSYFAHKKLARNNKSSGTLM"
     gene            complement(758..2563)
                     /locus_tag="PLA17_05520"
     CDS             complement(758..2563)
                     /locus_tag="PLA17_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84907.1"
                     /translation="MNRFILYRDFGDDTSRMTGNAGSGALAKPGGASTQTVRCGEYVL
                     SYYGNGYRHEGDTGIFIGGYIIPRNDVFKQYCGMSQHELVSHLYSNHGSSFTGYIKGI
                     FVVIILKAGCVELYFDRFGLFRSFYSSQGGQFMVSNTVSLLAWAGIELIPDKASLSMQ
                     ALFHRVPGRYTVYKDIFKTIGGDYFKLEGGTITHTRYYSPEYLMGLSQAAYDISLEEF
                     AAAFRNNVVNLNDFLKPESTLITLTGGKDSRTILAALLGAGIKPDAITYGNSLSRDAV
                     YASIVAHAAGIRHTITAPPDDREWFKNEAVNITGAGNPEISIHRSHRRHAFIVTAEQS
                     GSDSLFYAGYMGGELLMGIYYDDLIFTGFLRKLWDGYTVEELMAERLREYFITPVQEL
                     LAEVRERVRELACTDKGLSPEMREFHGLFEIGVPHHSQDITLASEFWNYPAPVFLDTD
                     FIELLFRSRDSFLFRDATTMNPFRRHSLFRINMGVQHILCPELDTVPFGKRGTYNIRE
                     YLRGPLAWSLVKGYRYLNDRIKYPPSFSYGKEYVDFLAGELEEARSGRSPAGRYYDLE
                     RAVRGLDGLEFPLQEKDLHKYSAIVAFHIMYRDKG"
     gene            complement(2568..3626)
                     /locus_tag="PLA17_05525"
     CDS             complement(2568..3626)
                     /locus_tag="PLA17_05525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPK84908.1"
                     /translation="MRLALAGDIAFSGLISSSSEANGERFRWLEDILSGCDGLIANLE
                     APVKADEKNSSKKAFLYADHEVTSDLLRRLNVICVSLANNHILDCGREGLANTIRLLD
                     DAGIYHSGAGLTEEQAAPVIFTVAGKRVAFASYNHSSTNPGREAYPDLFLNSFDLAHA
                     MAEVRNLKARAEIVVVSIHWGTDYSFFHEPWQRKTAEKIAEEGADIIMGHHSHTLQAF
                     EIIGSTQVFYGLGSLAFGDFEKNGKMYALFRRTKHSALFFTDPNRRLLEAVATHELKG
                     NYIVAGSKDIMKRNDRLLRLNRIREKNNYFSAMLRFHEKVLSRIYEYFFGYYMNPLAR
                     LFQFRNFRKVGRLLRQQP"
     gene            complement(3629..4888)
                     /locus_tag="PLA17_05530"
     CDS             complement(3629..4888)
                     /locus_tag="PLA17_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84909.1"
                     /translation="MNRRIRYTWQFYFVMAIPVINAIASNTTEYFSSDILNIGNLRAL
                     IIGLFAAWFMFTRHPNDSLSRFTLYYLGYYFLLALFSTDRFTSGTLYLKFLLGVIMMP
                     IGYYYIKSIARLEILLKVLLFTLGLHILNIIIVNIFQLGTSDYLDETFYFGAGRVNIT
                     KNILILVFMLPVTMLFIRKYRRIVFILYIVAFLIAIVGIKRSELISAAAGVVTYLAVK
                     QKFSLLLRTAVITSVSLLLIVVMFQGFADLFMSRFEAREERVELTEETIETEGRLSET
                     ETVFSAWSAGSFKHKLVGSEVFNDRLYFDTSRMLHTDYMIILNGSGLIGLFLWFYLFY
                     RIIKEKNKYYRLLRNHILFREMNAVFWVLLVVQLVLSVSGTVYAIELRSLIFLFWGAF
                     IGTMKGYLQDKGPVVNWSRSVVLTGKS"
     gene            complement(4881..5237)
                     /locus_tag="PLA17_05535"
     CDS             complement(4881..5237)
                     /locus_tag="PLA17_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84910.1"
                     /translation="MTASSVLFVIRSYSRTGAQPIHFFEIIDYLAGVFSIHVLELIHG
                     KPGTRQKREAVRLTKAHGLSHIITAINKAEAITDAIGKLITMNPVYPAKEHVSGFLWK
                     NRASEYEKLISFLAGE"
     gene            complement(5242..6447)
                     /locus_tag="PLA17_05540"
     CDS             complement(5242..6447)
                     /locus_tag="PLA17_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPK84911.1"
                     /translation="MRNKIIMLFLLILALPAIGQVDVDCEPVVLQKIDSVINYLRVGQ
                     TDTYDPVEVFIYHFDGVSEYPVIDKLALPSREPVNRQNYLFYEPSFKVKYVLQEWDGN
                     EFHDRSRTNYYYRPDRRLEREVFSRFDGSLWIPYQQHIYNYDDQNVIRTYLRQMMYTP
                     GIWTDFSYKNYIYDTQGRLIERNEQRIADGVIFWADLFEYNEANMVDVRVRQTLKYNP
                     QIRTYDLVNLERRRYSYNRYGNLESYLTDTWTNGSWVHAGKTRYFRSFILDKEIPICF
                     RGRTMIVPVRVAVRLFHVGAVPGRCECLTDGQDDGNNVRSNGKRRNNGLTLYPNPASN
                     SIRVIMPEGVSTYSDISLYDHNGTLVRRYPVGSQSDDLDISSLGAGTYYMVIIIAGEP
                     LMASFIKVR"
     gene            complement(6623..8047)
                     /locus_tag="PLA17_05545"
     CDS             complement(6623..8047)
                     /locus_tag="PLA17_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HPK84912.1"
                     /translation="MFWSFSDSMSTQLSQFIAGIILARILSPEEFGLIGMITVFVSIS
                     QSISDGGFGDALIRKKDASEADYSTAFYFNLIASAVIFAILWLTAPAVAGFYGRPELV
                     DIERVLAITILINAFGIVQRTQLTKNINFRMHMKVSLTASVVSGVAAIIMALTGFGVW
                     SLVWRSIIRSLIRAVMLWYTNRWVPFVRFSRESFRSLFSFGSKLLISNLIDTLYNNIF
                     LLIIGKFYSASQLGFFTRADQFSRLASRNLTNTVQRVSYPVLSLVQDENTRLREGYRK
                     LLNGTMYITFFLMLGMTAMARPMILTLIGEKWLPSVEYLQLLCLAAALYPLQVLNMNI
                     LSIKGRSDLLLKLEIVKKLLAIPVIMLGIFLGIRALLIGMVLHSLVSSFLNSYYSGRL
                     IYYPTADQLRDVMPSFLVALLASLCLLLLKFIPDLPPYVVFSMQVLMMLILAVVFGRF
                     FRLEGYIEIRNIITEKVPWLDRYL"
     gene            complement(8286..9266)
                     /locus_tag="PLA17_05550"
     CDS             complement(8286..9266)
                     /locus_tag="PLA17_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK84913.1"
                     /translation="MNLLTEKRVLVTGGAGFIGSSICHDLLERGNEVICLDNFATGKR
                     ENISDLRGNPKFKLIDGDIRDIETCHKAVKGVDVVLHQAALGSVPRSIADPATTNAVN
                     INGFLNMLIATRDAGIQRFVYASSSSVYGDSPELPKVEERTGKVLSPYAITKLVNEEY
                     ARVFGSLYGMETIGLRYFNVFGRRQDPNGPYAAVIPKFTMAFMKYESPVINGDGSNSR
                     DFTYIDNVVLANNLAATAENPAAINQAYNVACGDSVNLNEMTSMLRGYLAEKDPKIAD
                     VNPLHGPNRAGDIPHSMASIGKAVRLLGYKPMVRFREGLKRAVEWYVDNL"
     gene            complement(9382..>9665)
                     /locus_tag="PLA17_05555"
     CDS             complement(9382..>9665)
                     /locus_tag="PLA17_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84914.1"
                     /translation="APSPLGGWERYREWVARNLKYPEDILPVVRQEVVVSFTVQADST
                     ISDLKAVSSPGHSFTNEVFRLLIDGPQWVPARRGGKRMPEPVVLKIVFK"
     assembly_gap    9666..9765
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9766..10351)
                     /locus_tag="PLA17_05560"
     CDS             complement(<9766..10351)
                     /locus_tag="PLA17_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84915.1"
                     /translation="MKRDKRILIPRNEFEEEAGEGLGQLSREEAEADLRELKARIERR
                     IARPRAIWLSAAAAVVILLVGSALLVTLLRKRPLSDSQLAQRVPGKEGAAVADKEDMA
                     PAGEVITDTALIAMAEPIEKKGYESAVPAEDMSYAHARPAIAEIAEHEIETDATDEIF
                     ILAGEIIEDLEVADEQVVVLPVTEYQKVVEALQEE"
     gene            complement(10555..11172)
                     /locus_tag="PLA17_05565"
     CDS             complement(10555..11172)
                     /locus_tag="PLA17_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK84916.1"
                     /translation="MRPVFLRKKTETGSDEELLKRYRDEGDIRLAGQLYERYMNLVFG
                     VCLKYLEEREWAKDEVMNIFEKLVTVLPQQQEIGNFRTWLYVVTKNHCLMLLRSRKSE
                     AAHAEAMMNDPLFFMENTSGMHPMGDDEGGETDDPGGEDGMKRLEECIERLREEQRNC
                     IRLFFYEGCSYRQISERLGVDEMKVKSYIQNGKRNLKICMESMKV"
     gene            11613..>12837
                     /locus_tag="PLA17_05570"
     CDS             11613..>12837
                     /locus_tag="PLA17_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HPK84917.1"
                     /translation="MRTIASVIAFLLLTAAGPTTQILIQGTVTDSSGNPLSGVYVMIE
                     GAKNSVITGTDGSYSINAGLEAKVLVFCLRGMKEVKEPIMGRTIINVVLEPENEDITA
                     MNRKTGKEKKTDQTKEEYVVMEAEVYECLMEVVYDQVYAAPAGRNMKSSYYHLPPAEQ
                     NTESYAGISENGYRDPLREPYSTFSIDVDNASYSNVRRFINLGQKVPADAVRIEEMIN
                     YFRYDYPKPSGEHPFSLYTETGICPWNSNHYLLHVGLRGKDIDKSELPPSNLVFLIDV
                     SGSMDYPNKLPLLKSAFGLLVNELREEDRVAIVVYAGAAGVVLESTPGNRKETIMEAI
                     DRLNAGGSTAGGAGLTLAYKIAEKNFISGGNNRIILATDGDFNVGVSDNKSMQQLVEE
                     KRGLGIYMTVLGFGMG"
BASE COUNT         3402 a         3364 c         2701 g         3270 t
ORIGIN      
        1 ttcatgagag taccgcttga cttgttatta cgggctaatt tcttgtgggc aaagtacgaa
       61 tctcctatcc tgacacacct ccattcaaat tcatgtggca cgaactcctg gaaaattaca
      121 aacccacgct gcggattcag gtaagatgct ctgtattctc ccatcctctt cttcacaaat
      181 ccccttatgg taaatgccct gatgagctta ccggcaattg agcctttacg gagcttgggg
      241 cctgtcctta ctccgactcc cttgccgaag gcattgtgga tgtaatcata caactccttt
      301 ttatcatgga taaatcttac cccgtttcca gatgcgccaa tatttgtctt cgcaacaagc
      361 gggagcctga cccctgaccc ggcatactcc attgcctctt tcctgtcaaa gaaaacacgt
      421 gtctgaggat aaggtatatt attgatgttg agccagtcac tcaggaaccg cttattctcg
      481 taaatgagca cttcgttcag cgatgggtaa accggtactc ctgcatagtt gtaaaggata
      541 tgagcccttt catcatatag ccgcttgaat aactctgtac ggccaggagg gcgaagtaac
      601 accaggtcat aattcttcat gtggaatctc tccagccagt cagaagcagt aatgtcaata
      661 acctccgcct caacttcacc ctgtcgcctg ttgcaagcct ccacccatgg caggtgatca
      721 tcggccgcct catttcggaa tattacaatc ctctttctca tcctttatcc ctgtacatta
      781 tatggaacgc tacaatggct gaatacttat gcaaatcctt ctcctggagg ggaaattcaa
      841 gtccatccaa tccccggact gccctttcaa gatcataata acgacctgcc ggcgacctgc
      901 ctgaacgtgc ctcttccagt tctccggcca gaaagtctac atactccttc ccgtaggaaa
      961 aggagggcgg atatttaata cggtcattca gatacctgta tcccttgacc agactccatg
     1021 ccagcggtcc tctgagatat tccctgatgt tgtacgttcc tcgcttgccg aagggtacag
     1081 tgtcaagttc cgggcagaga atatgctgaa cgcccatgtt gattctgaat aacgaatgcc
     1141 ttcggaaagg attcattgtt gtggcatcac ggaaaagaaa actgtccctg ctgcgaaaaa
     1201 gaagctcaat gaaatctgta tccagaaaaa caggagccgg ataattccaa aattctgaag
     1261 cgagtgttat atcctgggaa tggtgtggca cccctatctc aaataaccca tggaattccc
     1321 tcatttcagg tgaaagaccc ttgtcagtac aggcaagttc cctgactctc tctctgacct
     1381 ctgccaaaag ttcctgaacc ggggtgatga agtattccct cagtctttct gccataagct
     1441 cctccaccgt atacccgtcc cacagtttcc ggagaaaacc ggtaaaaatc aggtcgtcat
     1501 aatagatccc catcaacaac tcaccaccca tgtaaccggc gtaaaagaga gagtcactgc
     1561 ctgactgttc cgctgttacg atgaatgcat gccgcctatg gctacggtga atgctgatct
     1621 caggattacc tgccccggta atgttgactg cctcattttt aaaccactcc ctatcatccg
     1681 gcggagccgt aattgtatgt ctgataccgg cggcatgagc aacaatagag gcatatacgg
     1741 catcccgtga aaggctgttt ccataggtaa tagcatcagg tttaatacct gctccaagca
     1801 gcgctgcaag aatcgtcctg ctgtcctttc cacccgtcag ggtgatcaat gtcgactccg
     1861 gttttagaaa atcattgaga ttgacgacat tattacggaa tgctgcagca aactcctcca
     1921 gggaaatatc ataggccgcc tgactcaacc ccatcagata ttccggtgaa taataacgtg
     1981 tatgtgtgat agtaccacct tccagcttga agtaatcacc tccgatagtc ttgaagatat
     2041 ccttgtaaac tgtgtaccgc cccggtaccc ggtgaaacaa tgcctgcatt gacagggagg
     2101 ctttatcggg tatcagctca atcccggccc aggccagcag actgacagtg tttgaaacca
     2161 tgaactgccc cccctgagag gaatagaaag acctgaaaag accgaagcgg tcaaagtaaa
     2221 gctcaacgca tccggcttta aggataatta cgacgaatat tcccttgata taaccagtaa
     2281 atgaacttcc gtgattgctg taaaggtgcg agacaagttc atgctgactc atgccacagt
     2341 attgtttaaa aacatcatta cgtgggataa tatagccacc gatgaagata ccggtatcac
     2401 cttcatgccg gtatccgttc ccataatagg agagcacata ttcaccgcac ctgacggttt
     2461 gggtactggc tcctccgggc ttcgctaaag caccagaacc agcattccct gtcatccggg
     2521 aggtatcatc cccgaaatca cgatatagaa taaacctgtt catagggtca tggctgttgc
     2581 cttaacaacc ggccaacttt cctgaaattc cgaaactgga acaaccgggc cagcggattc
     2641 atataatatc caaaaaaata ctcatatatc ctgctgagca ccttctcatg gaaccttagc
     2701 attgcagaga aataattgtt tttctccctg atgcggttca gtctcagcag acgatcattt
     2761 cgcttcatga tatccttact gccagctact atataattgc ctttgagttc gtgagttgca
     2821 acagcctcca gcagtctgcg gtttgggtct gtaaagaata gcgctgaatg ctttgtcctt
     2881 cggaaaagtg cgtacatttt tccgtttttt tcaaagtctc caaaagccag actacccaaa
     2941 ccgtagaaga cctgcgttga accaataatt tcaaaagcct gtagggtatg ggagtgatgg
     3001 cccatgataa tatcagcacc ctcttctgct attttttctg ccgtcttcct ctgccatggt
     3061 tcatggaaga atgagtagtc agttccccag tgtatactga caacgacaat ctctgccctg
     3121 gccttcagat ttctgacttc tgccatagca tgagccaggt caaatgagtt taggaagagg
     3181 tcgggatacg cttctctgcc cgggtttgtg gaactgtgat tataggatgc gaaagccacc
     3241 cttttccctg ccaccgtgaa tataaccggg gcagcctgct cctcagtcag ccctgcgccg
     3301 gaatggtaga tgccagcatc gtcaagcagc ctgatcgtat tggccaaacc ttccctgcca
     3361 caatcgagga tatgattgtt ggccagggag acacatatta catttaatct cctgagcaga
     3421 tctgaagtaa cctcatgatc agcatacagg aatgcttttt ttgaactgtt tttttcgtca
     3481 gctttcacag gcgcctcaag gtttgcaatg agtccatcgc atccggagag tatatcctcc
     3541 aaccacctga atctttcacc attggcttca ctactgctgc ttatcagccc gctgaaagcg
     3601 atatctcctg ccagcgcaag tctcataatc agctttttcc ggttaagacg actgatcggc
     3661 tccagtttac tacggggcct ttatcctgca ggtacccctt cattgtacca ataaatgcac
     3721 cccagaaaag gaagatcagg cttctcagct caatggcata aacggttcct gatactgaaa
     3781 gcacaagctg cactaccagc aagacccaga ataccgcatt catctcccgg aataggatat
     3841 ggttccgaag cagcctgtaa tacttgtttt tttccttaat aatcctgtag aaaagataga
     3901 accacaaaaa caatcctata agccctgaac cgttaagaat aatcatgtaa tcagtatgca
     3961 acatccttga ggtatcgaaa tagagcctgt cattgaaaac ctctgagcca accagtttat
     4021 gtttgaaaga cccggctgac caggcactga aaacagtctc tgtctcactc agacgaccct
     4081 ccgtctcaat ggtctcctct gtcagttcaa ccctctcctc cctggcttcg aatcttgaca
     4141 taaaaaggtc ggcaaatccc tggaacataa ccacgattaa taaaagtgaa actgaggtga
     4201 ttactgctgt cctcagaagc aagctgaact tttgtttcac tgcgagatat gtaactactc
     4261 cggcagctgc tgagataagc tccgaccgtt taattccaac aatggcaatg agaaaggcca
     4321 cgatgtacag aatgaaaaca atccgcctgt atttcctaat gaaaagcatg gtcacaggaa
     4381 gcatgaatac caggataaga atattcttgg tgatgttgac ccttccagct ccgaaataaa
     4441 aggtttcatc cagataatca gaagtaccca gttgaaatat gttgacgatg ataatattga
     4501 ggatatgcag tcccagggtg aacagaagta ccttcagcag tatctcaagc cgtgcaatgg
     4561 actttatgta atagtaccct atcggcatca taatgactcc aaggagaaac ttcaggtaga
     4621 gagttcccga tgtaaacctg tcagtggaga aaagggcgag aagaaaataa tagcccaggt
     4681 aatagagcgt aaaccttgat aagctatcgt tcggatgccg ggtaaacatg aaccatgctg
     4741 caaaaaggcc gataataagc gccctgaggt tgccgatatt tagtatatcc gaactgaaat
     4801 attcagtggt gtttgaggca atggcattaa ttacggggat cgccattaca aaataaaact
     4861 gccatgtata acggattctt ctattcacct gcaaggaatg aaatcagttt ttcgtactct
     4921 gaagcccggt tcttccacaa gaatccgctc acatgttcct ttgccggata aacgggattc
     4981 attgttatta actttcctat ggcatcggtg attgcctctg ccttattgat tgcggttatt
     5041 atatgactta aaccatgcgc tttcgtcaga cgtactgctt cgcgcttctg cctagtacca
     5101 ggtttaccat gtatcagctc aagtacatga atgctaaata ctccagccag gtagtctatg
     5161 atttcaaaaa agtgtattgg ttgtgctcct gttcttgaat atgatcttat gacgaacagt
     5221 acgctactgg cagtcattgg gttacctgac ttttataaat gatgccataa gtggctcacc
     5281 cgcaatgatt atcaccatat agtatgtacc tgcaccaagt gatgaaatgt caaggtcgtc
     5341 agactgacta cccacaggat acctcctgac aagtgtgccg ttatggtcgt aaaggctgat
     5401 atcactgtag gtggaaaccc cttcaggcat tatcactctt attgaattcg aggcgggatt
     5461 ggggtagagc gtcaggccat tatttcgcct cttaccatta ctcctgacgt tattcccgtc
     5521 atcctgaccg tcagtcaggc attcacatct tcccggtact gcacctacat ggaacagcct
     5581 gacagcaacc ctgacaggaa caatcattgt acggcctctg aaacatattg gaatttcctt
     5641 atcaagaata aaagagcgaa aatacctggt cttacctgca tgaacccagg atccgttggt
     5701 ccatgtatct gtcagataag actccaggtt tccgtatcta ttatatgaat atctccgcct
     5761 ctcaaggttg accaagtcat acgtccggat ctgcgggtta tatttcaatg tctgcctgac
     5821 cctgacgtca accatgttag cttcgttata ttcgaaaaga tcagcccaga aaataactcc
     5881 gtcagcaatc ctctgctcat tgcgctcaat aagtcttccc tgggtgtcat aaatatagtt
     5941 cttatatgaa aaatcagtcc agatccctgg tgtatacatc atctgtctaa ggtatgtacg
     6001 gattacattc tgatcatcat aattgtaaat atgctgctga tagggaatcc aaagactgcc
     6061 gtcgaaacgt gaaaacacct ccctttcaag ccgcctgtca ggcctgtaat aataatttgt
     6121 ccgtgaccgg tcatggaatt catttccatc ccattcctgt aaaacatatt ttaccttgaa
     6181 ggatggctcg tagaagaggt agttctgcct gtttaccggt tctcttgatg gaagggcaag
     6241 cttgtcaatg accggatatt ctgaaacgcc gtcaaaatga tatataaaaa cctccactgg
     6301 atcatacgta tcagtctgac ccaccctgag ataatttata accgaatcaa tcttctgaag
     6361 aacaacaggc tcacaatcga catccacctg tccgattgcc ggcaatgcca ggattagcaa
     6421 aaataacatt ataattttat tcctcatttt ctgatcgttg tgtttattat cttaaacgaa
     6481 gaagcttcga ttttgttctt ttatgattgg tttaaagatt atcagtactt cttttagctt
     6541 cctcagattt actttcttac tgtaaagtac aaataacggg taactcaccc tggcaatctt
     6601 ataaataatt ctattaccta tcctacagat accggtccag ccagggtacc ttttctgtta
     6661 ttatatttct aatttcgata tagccctcca gtctgaaaaa gcggccaaac accacggcca
     6721 ggataagcat catcaaaact tgcatcgaaa aaactacata aggtggaaga tcgggaataa
     6781 actttagtaa gagcaggcat aatgatgcaa gcaatgctac cagaaacgat ggcatgacat
     6841 cacgaagctg atctgcagtc ggataataaa taagcctgcc agaataatag ctgttcagga
     6901 aagatgaaac aagtgagtgc agaaccattc caatgagtaa cgccctgatc cccagaaata
     6961 ttccaagcat tatgacgggt attgctaaca gtttctttac tatctccagt ttcaataaca
     7021 ggtcagatcg gccctttatg ctcaggatat tcatgttaag gacttggagg ggatataatg
     7081 ctgccgcaag gcacagcaac tggagatact ctactgaagg gagccacttt tccccgataa
     7141 gtgtcagtat cattggtctc gccattgctg tcattcccaa catcaggaaa aaagtgatat
     7201 acatagtgcc attgagaagc ttcctgtacc cctccctgag tcttgtgttt tcatcctgca
     7261 caaggctcag caccgggtaa ctgacccgct gcaccgtatt tgtcaggttc ctcgatgcaa
     7321 gccggctgaa ctgatcagcc ctggtgaaga aaccaagctg ggaggcagag tagaatttac
     7381 cgataatgag caggaaaata ttattgtata acgtatctat aagattcgag ataagcagct
     7441 ttgagccaaa ggagaaaagc gagcgaaacg actcccgact gaatctgaca aacggaaccc
     7501 aacggttggt ataccaaagc ataacggccc tgatcaggct cctgataatg cttctccaca
     7561 caagactcca tacaccaaaa cccgtcagcg ccattatgat ggccgccact ccggatacga
     7621 cagacgcagt caggctcacc ttcatatgca tcctgaagtt tatgtttttt gtcagctgtg
     7681 ttcgctgaac aatgccgaat gcattaatta ggatagtgat cgctagaact ctctctatat
     7741 caaccaactc gggacgacca tagaacccgg ctactgcagg agcggtaagc cataatatag
     7801 cgaagatgac agcactggca atgagattga agtaaaatgc cgttgagtaa tcggcttcac
     7861 tggcatcctt cttgcgtatc agcgcatcac caaacccgcc gtcagaaata ctctggctga
     7921 tggagacgaa taccgtaatc atccctatca gcccgaactc ctcaggcgac agtatcctgg
     7981 ccagtataat ccctgcaata aactgcgaca gctgcgttga catactgtcg gaaaaactcc
     8041 agaacattcc ggagacagcc ttttgcttta atccttttga ggccaattga tgctgattgt
     8101 ggtatgacac tccctcttgc gtgacatact cttccttgga attttagaat cctccggttc
     8161 gtcagtcaca cagtatctgt gcctaacgta ctgtatatgt gattggtagg ccatgagcgg
     8221 cctatgcggt tgtgcccgtc tgcacaaata taagaaagaa catccgtcga gtcctgttaa
     8281 gaaactcaga ggttatcaac ataccactca acagcccgtt tcaatccctc tctgaaacgg
     8341 accatgggct tgtaaccaag cagcctcact gccttgccaa tgctggccat gctgtggggg
     8401 atgtcaccag cacggtttgg accgtgcagc ggattcacat ctgcaatctt cggatccttc
     8461 tctgcaagat aacccctgag catagatgtc atctcattga gattcaccga gtcgccacag
     8521 gcaacattat atgcctgatt tatggcggca ggattctccg cggtggctgc caggttgttg
     8581 gccagcacca cattgtctat gtaagtgaag tcacgcgagt tgctgccatc gccattgatg
     8641 accggcgact catacttcat gaaggccatg gtgaacttcg ggatgacggc tgcataggga
     8701 ccgttggggt cctgcctgcg gccgaagaca ttgaaataac gaagtccgat tgtctccatc
     8761 ccgtaaaggc tgccaaacac acgcgcgtac tcttcgttta ccagtttggt aattgcatag
     8821 ggcgaaagca ccttcccggt acgttcctct accttgggaa gttcggggga gtcaccgtaa
     8881 accgatgaac tggaggcata tacgaagcgc tgtatccccg cgtcgcgggt ggcgatcagc
     8941 atgttcagga aaccatttat gttgaccgcg tttgttgttg ccggatcggc aatgcttctg
     9001 ggcacactgc cgagagctgc ctgatgcagc accacgtcaa cacctttcac ggccttatgg
     9061 catgtctcaa tgtcacggat atcaccatca atcagtttga acttcggatt gcccctgagg
     9121 tcgctgatgt tttccctttt ccccgtggcg aaattgtcga gacagattac ttcgttgcct
     9181 ctctccagaa ggtcatggca gattgatgat ccgataaatc cggccccgcc ggtcaccagc
     9241 acgcgttttt cagtaagtag gttcatttaa ctaagaattt cgcaagttta tgaaaacagc
     9301 tcgcagagct tacagaattg taaacgcaat tgcctgtcaa aaaattggct gaaggccgtt
     9361 aagctttgta aaatggcatg tttatttaaa cacaatcttc aaaacaaccg gctccggcat
     9421 tctcttccct ccgcgtctgg ccggcaccca ttgcggccca tcaatcagca acctgaaaac
     9481 ctcattggtg aatgaatgac ctggtgaact tacagccttc agatcggaaa tcgtactgtc
     9541 tgcctgaacc gtaaatgaaa ccacaacctc ctgccgcacc acaggtaaaa tatcttcggg
     9601 atatttcagg ttccgggcaa cccactccct gtatctctcc cagccaccca gaggagaggg
     9661 tgccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttct tcctgcagag
     9781 cctccactac cttctggtac tcagtaaccg gtaatacaac aacctgttca tcagctactt
     9841 ctaaatcctc aataatttcc ccggctaaaa taaagatttc atccgtcgca tctgtctcta
     9901 tttcgtgctc tgctatctct gcaatcgcag gcctggcatg tgcataactc atatcctctg
     9961 caggcacggc agattcatat ccctttttct ctatcggttc cgccatggca atcagggcag
    10021 tgtcggtgat aacctctccg gccggcgcca tatcttcttt gtctgcaacg gcagctcctt
    10081 cctttccggg tactctctgt gccagttggg agtcagagag cggtcgcttt cttaacaggg
    10141 tcaccagcag ggctgagccg acaagcagga tgaccactgc ggctgcagcc gatagccata
    10201 ttgcacgcgg acgggcaatg cgccgctcta tccgcgcctt cagctcacgg agatcagcct
    10261 cggcctcctc ccggctgagc tgcccgaggc cttccccggc ctcctcctcg aactcattcc
    10321 gtggaattaa tattctttta tcccttttca tctccttcct ctttgtcctg caaaactcca
    10381 aactttcaaa acttcaaacc atcagacccg taaaactcca taactccaaa ccttcagacc
    10441 tgttaatcca gcaaacctcc atgcctgtaa acctccaaac cttcagaccc gccaaccttc
    10501 acacctccgt actttcagag ctgtaaacct tcaactctgc aaacctccag gtcatcagac
    10561 tttcatactt tccatgcata tcttcaggtt cctcttccca ttctgtatat acgatttaac
    10621 tttcatctcg tctacaccca gcctctcact tatctgccgg tagctgcacc cctcgtaaaa
    10681 gaacagcctg atgcagttcc tctgctcctc tctgagccgt tcaatgcact cctcaagtct
    10741 cttcatacca tcctccccgc cggggtcgtc cgtctcaccc ccctcatcgt ctcccatagg
    10801 atgcatacct gaagtatttt ccataaaaaa gagcgggtca ttcatcattg cttcagcatg
    10861 agccgcctcg ctcttccggc tcctcaacag catcaggcaa tgattcttcg tgaccacgta
    10921 aagccatgtc ctgaagttac cgatctcctg ctgctgcggc aacaccgtca ccagcttctc
    10981 aaatatgttc atcacctcat cctttgccca ctctctctct tccaggtatt tgaggcatac
    11041 gccgaagacc aggttcatgt atcgctcata caactgccct gccagcctga tgtcaccctc
    11101 atcgcggtaa cgcttcagga gctcctcgtc actccccgtt tcagtttttt ttctcaggaa
    11161 taccggccgc attcaaatcc gttttcggca gttgtcaatc agagtataaa cttacgaaaa
    11221 tcacccggtt cttatcagcc tggatagatc tgataggtca gttttgaccg gaacaaccgg
    11281 agaaagaata agggtagccc gaaggggccg atgaaatgtc tttcaggtct ctatggactt
    11341 tatccgtgta gccggcaaaa tggaaccagc cacccgacgg tgctgataaa aagtctctcc
    11401 ggatttttat cgaatctgcc agggccggcc ggcaaccagg aaatacccac acgccagaac
    11461 cgatatataa tctttccgga cctctataga ctctgccagg ggcgatcagc aaccagcccc
    11521 acccaccgga gccgccagaa aaattttcac aaacttttat ggaatctgcc cggccgctgc
    11581 atccttacca cagaatcaca aatcaaagaa ccatgagaac aatcgcatca gtaatcgcat
    11641 tcctgctctt aactgcagca ggaccaacaa cacagatcct cattcagggc acagtgaccg
    11701 acagctccgg caaccccctc agcggggttt atgttatgat agagggagca aaaaacagcg
    11761 tcataaccgg caccgacggc tcatactcca tcaacgccgg cctggaggcg aaagtccttg
    11821 tcttctgcct ccgggggatg aaagaggtta aggaaccgat aatgggacga accattatta
    11881 atgtggtact cgaaccggag aacgaggata tcaccgccat gaacaggaaa accgggaagg
    11941 agaaaaaaac agaccagacc aaggaagaat atgttgtgat ggaggctgag gtatacgaat
    12001 gcttaatgga agttgtttat gaccaggttt atgccgcccc ggcaggaaga aacatgaaat
    12061 catcatacta ccatctgccc cctgctgagc agaacaccga gtcatacgcc ggtatcagcg
    12121 agaacggtta ccgcgatccg ctgcgcgagc cttactccac cttctctatc gatgtcgaca
    12181 acgcctctta ttcaaatgtg cgccgtttca tcaacctcgg gcagaaggta cccgccgatg
    12241 ccgttcgtat cgaggagatg atcaactact tccgctatga ctacccgaag ccatccggcg
    12301 aacacccgtt ctcactctat accgagacag gcatctgccc ctggaacagc aatcactacc
    12361 ttctccacgt tggactcagg ggaaaggata tcgacaagag cgaactgccc ccctcgaatc
    12421 ttgtgttcct tatagatgta tcaggatcga tggattaccc gaacaagctc cccctcctga
    12481 aatctgcttt cggactcctt gtcaatgaac tcagggaaga ggatcgtgtg gcaatagtcg
    12541 tctatgccgg cgctgccgga gtggtgctcg agtcaacccc cggcaacagg aaggagacca
    12601 tcatggaagc cattgaccgt ctgaatgccg gtggctccac cgctggcggc gcaggtctca
    12661 cgctggccta taaaatcgct gaaaagaatt ttatcagtgg tggcaacaac cgcatcatcc
    12721 tggcaaccga cggcgacttc aacgtcgggg tttccgacaa taagtcgatg cagcagctgg
    12781 tggaggagaa gcgcggcctc ggcatctaca tgactgtcct cggattcggg atgggaa
//
LOCUS       DAOPBK010000069        12765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4895_length_12765_cov_4.807553, whole genome shotgun sequence.
ACCESSION   DAOPBK010000069 DAOPBK010000000
VERSION     DAOPBK010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4895_length_12765_cov_4.807553"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLA17_05575"
     CDS             complement(<1..572)
                     /locus_tag="PLA17_05575"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPK84918.1"
                     /translation="MNTIGTIFRVSLFGESHGPAVGVVIDGCPAGLHYWREELMADLS
                     RRKGLLPGTTTRRETDEPEVISGIYNDYTTGAPLVICTQNKDFNPGDYEQFTSIPRPG
                     HADFTAGFKYKGFSDMRGGGHFSGRLTWGIVTAGYFARKIVSPAIITATLVEAGGETD
                     IPAAIAKAMETNDTIGGVVECVVKNVPKGLG"
     gene            complement(589..1635)
                     /gene="aroB"
                     /locus_tag="PLA17_05580"
     CDS             complement(589..1635)
                     /gene="aroB"
                     /locus_tag="PLA17_05580"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPK84919.1"
                     /translation="MKRIRINGPAGESLILSGGTVDDVVTLLPKKGLFIITDRNVKKL
                     YGKQFPHGEVIAIEPGEKSKRLKVVEEVCSRMLEAGADRSSFILGFGGGVVCDIAGMV
                     ASVYMRGVRHAFVSTTLLSQVDASIGGKTGVNLGEYKNTIGTFRQPEFVLCDHEMLKT
                     LPEAEVRSGMGELFKYAVIRDRNLFFDISASAGQIIAGNMAVTGDLILRAVRIKAGVA
                     RDDPFEKGKRRILNFGHTFGHVIETHYGLPHGVAVANGMVIAAELSVWKGEMAHSELS
                     LLQTTLAKAGLLTGHKLPANVVSMISRDKKSESDRVNFVLLRSIGRTVIRRISTFELQ
                     TFVNYYNEKESHSQ"
     gene            1750..2394
                     /gene="nth"
                     /locus_tag="PLA17_05585"
     CDS             1750..2394
                     /gene="nth"
                     /locus_tag="PLA17_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPK84920.1"
                     /translation="MKTAERYSGILEWFKTNMPLPETELVYSDPFQLIVAVILSAQCT
                     DKRVNMITPALFARYPDAFSMAKATQEEIFGLIKSCSYPNNKAKHLLGMAEMIVNRFN
                     GKVPSTHEELTMLPGVGRKTANVIASVVWNSPVIAVDTHVFRVARRLGLTPGARNPLE
                     VELRLTREIPEELRPKAHHWLLLHGRYTCTARKPGCQECGLKQWCAWYASQTGK"
     gene            2490..4583
                     /locus_tag="PLA17_05590"
     CDS             2490..4583
                     /locus_tag="PLA17_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK84921.1"
                     /translation="MEKRKEPVNEPEHSEEYLTSLRNLAEIPELAFNSTNQLITLSNL
                     ETQKYIEVNQAFLDTTGYRREEIIGKTSDDIQLYVDLIQSRKYMRLISRLKQVSDLEI
                     KLRMRSGDERTFLFSARTIFMGDEIFLITYYNDISVLNDGFARAESEKILREIFDSMS
                     SYAIILRREGDDKHFIIDFNYRAEDVEQIDRKDVLGKEIGETPFARNETFLSLLENLE
                     SSHRPYKTPVSPDGSDDSGFYIGLLLSSGDILVTWEAGKAQKERESEILKQGIVFETF
                     AELLPEMIMEIDVEGHVTFMNTRGMMTLGFDNNDLANGITLSSLFMPEDLERLMSELS
                     GLTSPGQITFDEYVLVNKLKRRVTVLFHAGPIIIRNRVTGYRCVLTDVSKRKEYEQKL
                     SNEKALLEHLIDAAPEAIVLTDFDGRILRINHGFTRLFGYDADEAKNRLIDDLVVPEE
                     LRDEAIKIDETALDTGTGYLETIRRDKQGNRINVSLIASSVISNDMTIAFLGIYRDIS
                     KEIKSRLMQEIQYNISTLALQDSDIFDIYPTIVKEIGKLWNVNNFFIALYNREKDTLT
                     FPFFADERDHFQETAAKNTLTGWVINNNKAVLLDENDILMIEKTGAISLVGSPCKIWM
                     GVPLRMDDEVIGAICLQDYKKIDAFNNEDLQVFEFIANQIALTLQRRKMLDNLITAAR
                     EPRKLLSPSSSSCLP"
     gene            4571..5674
                     /locus_tag="PLA17_05595"
     CDS             4571..5674
                     /locus_tag="PLA17_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK84922.1"
                     /translation="MSTMSHEIRTPLNEVIGITNLLWQSDPREDQMDYINTLRFSANH
                     LLTLVNDILDYNKMEAGKIVFEKTEFDLTSMLEDMKRSYSHRALEKGIYFALEKSPDL
                     PVTLIGDPIRLNQILSNLLSNAMKFTSHGGVTLKASLKERQEGRAVIEFSVADTGIGI
                     PREKQDEIFESYAQASSDTTRKYGGTGLGLAICKRLVDLQGGTIGVTSEPDNGSVFTF
                     SIEYSMPAEEPRTAESGTASDSMNALVGKKILVAEDNKINFFVANKFLESWGVKVTHV
                     ENGALALEEIKKQDFDLILMDLHMPVMDGIEATRIIRSSADKKISQIPIVALTAAVMS
                     EAHDKIEDLAINDYVLKPFKPKDLYNRIVKNAR"
     gene            complement(5700..6902)
                     /locus_tag="PLA17_05600"
     CDS             complement(5700..6902)
                     /locus_tag="PLA17_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK84923.1"
                     /translation="MKKILFSLVVLLVSQVMSGQTGYETLLRAITLADRGEAEKAAAL
                     LAGTDEINGDVNLLSVRGDIYLRASMIREARADFMRAENLSQGSGLYGLARCAAADGD
                     AKGAVSLLEAHLKSAFRKSEPVIMLDPSFTSLSSSPEWRALWKKEWYRADERKSWEID
                     YYLKSGRNDMAAGVYAGLASLYPEKPVTEYCHARILMSNGKYREAADILETITGNSDA
                     PAGWLYTLAEARECEGNAYAAATIYDRMIRARVADPALLLKRARMLIRSGDREAAKKE
                     MQRYLSIDPDNSEALGLIGKTYAEEGAIYEALPYLNDNVEKHPGEASAFRLRGDAWLA
                     ARTWDRAAEDYTMSLDLDPENGNVNLNLGIALINSGKSDDACYYLRKARSLGVKEATE
                     YLARYCIR"
     gene            complement(6908..9517)
                     /locus_tag="PLA17_05605"
     CDS             complement(6908..9517)
                     /locus_tag="PLA17_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPK84924.1"
                     /translation="MIKRRIFYALALLLAVTFSATATHNRAGEITYRQISDLTFEVTV
                     TTFTYTLSKADRPSLDVEWGDNSITNVARISETILPNDYKKNVYVAEHTYPGPGVYRI
                     VVQDPNRNLGVENIPNSVNVVFSISTILIVNTGIGRNSTPLLLNPPYDKAALGQVFIH
                     NPAAFDPDGDSLSYKLTVCTREDGKPIQNYTLPPASRKFYVDSISGDLVWDAPTAIGI
                     YNVAMEIQEWRAGIKIGVVVRDMQIEVYETDNNPPVTSELTDLCVEAGRQVSLDIHAT
                     DVDLDSITLMATSGIFTSPDCPAEFTTLSSVAGHTTARLSWTPCHNMVRHQPYDIVIK
                     AEDSNRELQLVDIDNMSIKVLGPSPELLSAVPQGKVVQLSWAGYVTDQVAGFSIYRRE
                     GASALQVDTCFDGIPPSAGFVKVGYAGGAGTVSFTDTDDALEAGTEYAYRIVAVYPNG
                     TESKPSNEIVTSLITGIPLITNVSVRNTHHVNGSMTIAWQKPARLDTIPANGPYEYLI
                     YRAEGIAGTSWQLIHTLPTTDLNDTVYIDTLINTLDKGWVYRIELYNNAPGNRFLTGD
                     PGIASSLFLTALPGDRKVRFGITRNVPWINTSYDFYRYNGSAWEEAGSTNQLTFTDGG
                     LVNGTEYCYYVVSRVGYQGAGTPKNLINLSQRACAVPVDNEPPCTPAITVSSQCDSLY
                     NTVRWTFEDPLCLDDVAGYKLYYKQTTEEDLALLTTIDDRDVRKYVHSLPDYVAGCYA
                     ISTFDLIGNESPLSTMICVDSCNFYEIPNVFTPNGDDFNDWLLAKTSALVEKVEFRLF
                     NRAGVLVFSTQEPRLNWDGTYKGKVVPTGVYYYDCEVFERRISGLEQFHLSGFIHIYS
                     EKGAGPGIIEGKK"
     gene            complement(9621..10082)
                     /locus_tag="PLA17_05610"
     CDS             complement(9621..10082)
                     /locus_tag="PLA17_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84925.1"
                     /translation="MKTITLTLLLLIHPVHVSLTGIDYDGVKRVWSVFVKVWSDDLEA
                     DMNLGMSDGSEITGSTEERYFLYLTDRVMIMEDGKPVSMELISAGVDGLEHRFTLRAK
                     GRKGVKDVTVMNRIMTRLYEDQANMLLFGYEGMEEGYRFTVTDTLKSYLVK"
     gene            complement(10111..11568)
                     /gene="pyk"
                     /locus_tag="PLA17_05615"
     CDS             complement(10111..11568)
                     /gene="pyk"
                     /locus_tag="PLA17_05615"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPK84926.1"
                     /translation="MARNLLKKRTKIVATISDRNCEPAFLRRLHEAGMDVVRVNSAHL
                     DADGVRKIVANVRSISDHIAILLDTKGPEIRTTLCSEPLTFNKGDTVRVAGDSAAGTT
                     REVINVNYPDIAEYVPEGAIILIDDGEIEIKIIGREGDALIGKIENDGVLGSRKTVNI
                     PQVRINLPSVSERDLGFIDLAVELDIEFIAHSFVRSKDDVLAVQHLLDQRSSAIKIIS
                     KIENQQGVDNLEEIMDHSYGIMVARGDLAIEVPFERIPGIQRNIIARCIEKRKPVIVA
                     TQMLHSMIQNPRPTRAEVSDVANAVYSQTDALMLSGETAAGKYPVEAVRTMTRIASEA
                     EKEKDQYIEMPAGTLSGPVSAYLAKVAVKTSVRIKARAILADTINGTAIRNMAAFRGI
                     NPVIAHCYSHRTMRELMLSYGVIPIFIEKKMSVDEFIQVSVRELLKNFSMKESDIVVA
                     LAGNFSRGTGFSFIEVGTVGYLMERAGVTRQELMA"
     gene            complement(11576..12004)
                     /gene="aroQ"
                     /locus_tag="PLA17_05620"
     CDS             complement(11576..12004)
                     /gene="aroQ"
                     /locus_tag="PLA17_05620"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPK84927.1"
                     /translation="MTIEIINGPNLNLLGKREPAIYGTGGFEAVLGRLKERFPQVSFR
                     YSQSNIEGELVNRIQEAGSSSDGIIINPGGYTHTSVAIADAIAAITIPVIEVHISNLL
                     TREEFRHSSLTGRYCRGTIMGLGTDGYRLAAEALIEILKK"
     gene            12109..>12765
                     /locus_tag="PLA17_05625"
     CDS             12109..>12765
                     /locus_tag="PLA17_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPK84928.1"
                     /translation="MTISWTDAIRGFGRYLRIERSLSPNSVAAYLADVRRLHDWVESH
                     RPGSGPSELTPGDLSDFVAFIAARNDAVRTQARVISGIRSFYGYLLVENLVTDDPLML
                     PELPKTGMKLPEVLTVDEIDRMVASIDLSQPAGHRNRAIIETLYGCGLRVSELVNMRL
                     TDIHRGEGFVTVTGKGNKQRLVPIGSVTLKEIDNYMERRMMMPAIIDTNILFLNRRGR
                     R"
BASE COUNT         3066 a         3353 c         3151 g         3195 t
ORIGIN      
        1 ccgagcccct tcggaacatt tttcaccacg cactccacca ctcctccgat ggtgtcattg
       61 gtctccattg cctttgcaat agccgccggg atatccgtct cccctcccgc ttccaccagg
      121 gtggctgtta ttattgcagg cgacacgatc ttgcgggcga agtaaccggc agtaacgatg
      181 ccccatgtaa ggcggcccga gaaatgccct cctccgcgca tgtcgctgaa gcccttatac
      241 ttgaacccgg ctgtgaaatc agcatgaccg ggccgcggta tactggtaaa ctgctcatag
      301 tcgcccggat tgaagtcttt gttttgggtg cagatcacca gcggggcgcc tgtagtatag
      361 tcattgtaga taccgcttat cacctccggc tcatccgtct ccctgcgggt ggttgttccc
      421 ggcagcaatc ccttcctcct ggagaggtct gccatgagct cctcacgcca gtagtgcaga
      481 cccgcggggc aaccgtcaat caccactccc actgccgggc cgtgcgactc cccgaacagt
      541 gataccctga aaattgttcc gatcgtgttc atttttcaaa taacataatt actgactatg
      601 actctccttt tcgttgtaat aattgacaaa tgtctgtagt tcaaaagttg aaatccttct
      661 tatgaccgtt cttccgattg atctcaataa aacgaaattc acccggtctg actcactttt
      721 cttgtccctt gagatcatgc tgaccacatt cgccggcaat ttatggccgg tcagaagacc
      781 ggctttcgcc agtgttgtct gcaggaggct gagttcagaa tgagccattt ctcctttcca
      841 tactgacaat tccgcagcaa tgaccattcc gtttgccaca gccactccgt gagggagccc
      901 gtaatgtgtt tcaattacat ggccgaaggt atgcccgaaa ttgagtatcc ttctcttccc
      961 tttctcgaac gggtcatctc ttgccactcc ggcttttatc ctgacagccc tgaggatcag
     1021 gtcaccggtt accgccatgt ttcctgcgat gatctgtccg gctgaagccg aaatatcaaa
     1081 gaaaaggttc ctgtcacgaa tgactgcata cttgaacagc tctcccattc ctgacctcac
     1141 ctctgcctca ggcagtgttt tcagcatttc atggtcacag agaacgaact ccggctggcg
     1201 gaatgtaccg atggtattct tgtattctcc caggttcaca cccgtcttcc ctcctatgct
     1261 tgcgtccacc tgtgagagca gtgtggtgga aacgaatgcg tgcctcaccc ctctcatata
     1321 aactgaagcc accatgccag caatatcaca taccactcct ccgccaaacc cgaggatgaa
     1381 tgatgaacgg tcggcgcctg cctcaagcat cctgctgcag acctcttcca ctaccttcag
     1441 cctcttgctc ttctcccccg gttcaattgc aattacctct ccgtgaggaa actgtttccc
     1501 gtacagcttc tttacattcc ggtcagttat aatgaagagc ccctttttcg ggagcagagt
     1561 gacaacatca tccactgttc ccccggataa aataagggat tcccccgcag ggccgtttat
     1621 tctgatccgc ttcattccgg cacaaaataa aaaacaaaac cggttttcaa catgatttta
     1681 ttcagatagt ggtacttttt cggtaaaaaa taaaaatagt aaattcatcc cctgaaacag
     1741 aagagcagcg tgaagacagc agagagatac agcggcatcc tggaatggtt taagacgaac
     1801 atgccactgc cggagacgga gctggtatac agtgatcctt ttcagcttat tgtggctgtg
     1861 attctctcgg cgcagtgcac tgacaagagg gtgaacatga tcactcccgc tctttttgct
     1921 cgttatccgg atgcgttttc aatggcaaaa gcaactcagg aagagatatt cggactgata
     1981 aagagttgtt cctacccgaa caacaaggcg aagcatctcc tggggatggc tgagatgatt
     2041 gtaaaccgtt tcaatggcaa ggttccctcg actcatgagg aactgaccat gttgcccggg
     2101 gttggcagaa agacggctaa cgtcatagcc tcggtggtat ggaacagtcc ggtcattgcc
     2161 gttgacacgc acgtcttcag ggttgcccgt cgtctggggc tgactccggg ggcgaggaac
     2221 ccgcttgagg ttgagctcag gctgacacgt gagatacctg aagaattaag gccaaaggca
     2281 catcactggc tgcttctcca cggccgttat acctgcacgg cacgaaaacc cggatgccag
     2341 gagtgcggtc tgaagcagtg gtgcgcatgg tatgcttctc aaacaggaaa ataagtagac
     2401 ggggcagtcc gttatacatc atatatatat gagtaaattt gttcagttgg accgctattc
     2461 cccgtttaat gtacacacat gacatcagga tggaaaagag aaaagagcca gtcaatgaac
     2521 cggaacactc cgaggaatac ctgacctctc tcagaaatct tgcagaaatc cccgagctgg
     2581 cgttcaacag tacgaaccag ctgatcaccc tgagcaacct cgagactcag aagtacattg
     2641 aggtcaacca ggctttcctt gataccacag gttaccggag ggaggagatc atcggcaaga
     2701 cctccgatga cattcagctt tatgttgacc tcattcagag ccgcaagtac atgaggctca
     2761 tctccaggct gaagcaggtg agtgatctcg agatcaaact acggatgcgt tcgggtgatg
     2821 aaagaacgtt tctcttcagt gcccgtacta tcttcatggg cgatgagata ttcctcatta
     2881 catactacaa tgacatcagt gtactgaacg acggctttgc acgggctgag tcggagaaga
     2941 tactgaggga gatattcgat tccatgagca gctatgccat catcctcagg agagagggtg
     3001 acgacaagca tttcatcatc gacttcaact acagggccga agatgtggag cagattgacc
     3061 gcaaggatgt cctcggcaag gagatcggtg aaacaccttt tgcaaggaac gagacattcc
     3121 tctcgctcct tgaaaacctg gaatcaagtc atcgtccgta caagacaccc gtttcaccgg
     3181 atggcagtga tgactcaggt ttctatatcg gactattgct ctcttcagga gacatacttg
     3241 ttacctggga ggccggtaaa gcacagaagg aaagggagag cgaaattctt aaacagggaa
     3301 tcgttttcga gacctttgcc gaattgctcc ctgagatgat aatggagatt gacgtcgagg
     3361 ggcatgtcac tttcatgaac acccggggaa tgatgacact ggggtttgac aacaacgatc
     3421 ttgccaacgg aatcacactc tcgtcgctct ttatgcccga agaccttgaa aggctgatga
     3481 gtgagctctc cggcctgacc agtcccgggc agatcacctt tgatgagtat gtcctggtaa
     3541 acaagctgaa aaggagagtg acggtactct ttcacgcagg tccgattatc atcaggaaca
     3601 gggtaaccgg ttacaggtgt gtcctgactg atgtaagcaa gcgcaaggaa tatgaacaga
     3661 agctctcgaa tgagaaggct ctcctggagc atctgattga cgccgctccc gaagccatcg
     3721 tgctgaccga ctttgacggc cgcatcctca ggatcaacca tggttttacc cggctcttcg
     3781 gctatgatgc cgatgaggcg aagaacaggc tgattgatga cctcgttgta ccggaggagc
     3841 ttcgcgatga agctataaaa attgatgaaa cagcccttga caccggaaca gggtaccttg
     3901 aaacgataag gagggacaag cagggaaaca ggataaatgt ttcactgatt gcctcgtcag
     3961 tcatctcaaa cgacatgacc atcgccttcc tgggtatcta ccgcgatatc tcaaaggaga
     4021 taaagagcag gctgatgcag gagattcagt ataatatatc gacattggcg ttgcaggact
     4081 ctgatatctt tgacatctat ccgaccatag taaaggagat aggcaagctc tggaacgtca
     4141 acaacttctt catagcactt tacaaccggg aaaaggatac tctcactttc ccattctttg
     4201 cagatgagcg tgaccatttc caggagacgg ctgcaaaaaa caccctcacc ggatgggtta
     4261 tcaataacaa taaggctgtg ctcctcgatg agaatgacat tctcatgata gagaagaccg
     4321 gtgccatcag cctggtcggg tcaccatgca aaatctggat gggcgtgcca ctcaggatgg
     4381 atgacgaagt gataggagcc atctgcctgc aggactataa gaagattgat gccttcaaca
     4441 atgaagacct gcaggtgttt gaattcatcg ccaaccagat tgcacttacg ttacagcgtc
     4501 gcaagatgct tgacaatctc atcactgccg caagagagcc gaggaagctg ctttctccaa
     4561 gcagcagttc atgtctacca tgagccatga gatacgcaca ccgctgaatg aagtgattgg
     4621 cattaccaac ctgctgtggc agagtgatcc gcgtgaagac cagatggact acattaatac
     4681 cctgcgcttc tctgccaacc acctcctcac acttgtaaat gatattctcg attacaacaa
     4741 gatggaggct ggcaagatcg tctttgaaaa gaccgagttt gacctgacat ccatgcttga
     4801 agacatgaaa cggtcataca gccacagggc cctggagaag ggtatttact ttgccctgga
     4861 gaagagtcct gatctgccgg tgaccctaat aggtgacccc atcaggctta accagatcct
     4921 ttccaacctg ctttcaaacg ccatgaaatt cacatcgcac ggaggagtga cgttgaaagc
     4981 ttctttaaag gagcgtcagg agggcagggc agtaatcgag ttctcggttg ctgataccgg
     5041 tatcgggata ccccgcgaga agcaggatga gatctttgag agctatgcac aggcgtcgtc
     5101 tgacaccacc cgcaagtatg gcggaacagg gctgggcctt gcaatatgca agaggctggt
     5161 tgatctccag ggtggcacca tcggcgtgac cagcgagccc gacaacggtt cggttttcac
     5221 cttctccatt gagtacagca tgccggctga agagcccaga acagcagaga gcggaaccgc
     5281 atcagacagc atgaatgctc ttgtcgggaa gaagattctc gttgcggaag acaataagat
     5341 caacttcttc gttgccaaca aattccttga aagctggggt gttaaggtta ctcatgttga
     5401 aaacggggca ctggcgctgg aggagatcaa aaaacaggat ttcgacctca tcctcatgga
     5461 cctccacatg cctgtcatgg acggtattga ggctacgagg atcatcagaa gttcggccga
     5521 caaaaagata agccagatac ctattgtggc tctcaccgct gcagtgatgt cagaggctca
     5581 tgacaagata gaggacctgg ccataaatga ctacgtgctg aagccgttca agcccaaaga
     5641 tctttataac aggatagtga agaacgcaag ataggcgctg caatggcgcc ttttttattt
     5701 tacctgatac agtatctggc caggtattcg gttgcttcct tcactccaag gctccttgcc
     5761 ttgcggaggt aatagcaggc atcgtcactt ttgccgctgt ttatgagcgc tatcccgagg
     5821 ttgaggttca cattgccatt ctccggatcc aggtcaaggc tcattgtata atcttcggct
     5881 gccctgtccc aggtacgcgc tgcaagccac gcgtcgccac gcagcctgaa ggcagaggcc
     5941 tcaccgggat gtttctcaac attgtcattc aggtatggca gcgcttcgta tattgccccc
     6001 tcttccgcat atgtttttcc gatcagcccg agcgcctcgc tattgtcagg atcaatggaa
     6061 agatatctct gcatctcctt cttcgcagcc tccctgtctc ctgacctgat cagcatgcgt
     6121 gcccttttga gcagcaatgc agggtcagcc acacgtgccc tgatcatcct gtcatagatg
     6181 gttgccgcgg cataggcgtt tccctcacat tcacgggctt cagcaagggt ataaagccat
     6241 cctgccgggg catcactgtt cccggtgatg gtttcaagga tatctgctgc ctcacgatat
     6301 ttgccgttgc tcatgaggat gcgggcatga cagtattcgg taaccggttt ttccggatat
     6361 agtgatgcca gcccggcata cacccctgcg gccatgtcat tgcgacctga tttcaggtaa
     6421 tagtctatct cccagctttt cctctcatca gccctgtacc actccttctt ccatagcgct
     6481 ctccactcag gcgaggaaga gagagaggtg aatgagggat caagcatgat caccggctca
     6541 ctcttcctga aagccgactt aaggtgggct tccagcaatg agacggcacc ctttgcgtca
     6601 ccatcggctg cggcacacct cgcaaggcca tagagccctg atccctggct aagattctcc
     6661 gctctcatga agtcggctct tgcctccctg atcattgatg ccctgaggta gatgtcaccc
     6721 ctgactgaca ggaggtttac atcaccattg atttcatctg ttcctgcaag gagtgcggca
     6781 gccttttctg cttctcccct gtcggcaagg gtgattgccc tgaggagggt ttcataaccc
     6841 gtctggcctg acataacctg tgagaccagc aaaacaacca ggctgaagag tatctttttc
     6901 attgctttca ttttttacct tcaataatac cgggccctgc acccttttca gaatatatat
     6961 ggatgaaacc gctcaggtgg aactgttcca gtccgctgat ccttctctca aacacctcac
     7021 agtcatagta ataaacacct gtcggcacca ccttgccctt atatgttccg tcccagttca
     7081 gtctgggctc ctgggtgctg aacaccagca ccccggccct gttgaagagc ctgaactcaa
     7141 ccttctcaac cagcgcggag gtcttcgcaa gcagccagtc attgaaatcg tcaccgttgg
     7201 gtgtgaagac attaggtatc tcgtagaagt tgcaggagtc aacacagatc atcgttgaca
     7261 gcggactctc attaccgatc aggtcaaatg ttgagattgc atagcagcct gcaacatagt
     7321 cagggagtga gtggacatat tttctcacat ccctgtcatc tatggttgtc agcagcgcaa
     7381 gatcctcctc tgtggtctgt ttgtagtaga gtttgtaccc tgccacatca tcaagacaaa
     7441 gcggatcctc aaatgtccac ctgactgtat tgtaaagact gtcacactgt gatgacaccg
     7501 ttattgccgg cgtgcatggc ggttcattgt caaccggcac ggcacatgcg cgctgtgaga
     7561 ggtttatcag gttcttcggt gttccggcac cctggtatcc tacccttgaa acaacataat
     7621 agcaatattc ggtgccgtta accagcccgc cgtcagtaaa ggtcagctgg tttgtcgaac
     7681 ctgcctcctc ccatgcagag ccgttgtatc tgtaaaagtc ataggaggtg ttgatccatg
     7741 gcacattccg ggtaatcccg aagcgaacct tcctgtcgcc gggaagtgcg gtgaggaaga
     7801 gcgaggaggc tatgccaggg tcacctgtca ggaacctgtt tcccggggca ttgttataca
     7861 gttctatccg gtacacccag cccttgtcaa gcgtatttat cagggtgtca atataaaccg
     7921 tgtcattcag gtctgttgtg ggcagggtat gtatcagctg ccatgaagta cctgctattc
     7981 cttccgcacg gtatataaga tattcataag gaccgttggc gggtatggta tcaagacgtg
     8041 ccggcttctg ccatgcgatt gtcattgatc cgttcacgtg atgggtgttc cgtaccgaga
     8101 catttgtgat cagggggata cccgttatca gtgacgtgac tatctcattc gatggtttgc
     8161 tctccgtgcc gttcggatag acagccacga tcctgtatgc atattcggta ccggcctcca
     8221 gggcatcgtc agtatcggta aagctaacgg ttcctgctcc tccggcgtat ccgaccttta
     8281 caaatcctgc tgacggcggt atgccgtcaa agcaggtatc aacctgcagg gcggatgcac
     8341 cctctcgcct gtagatactg aatcctgcca cctggtcagt gacataccct gcccaggaaa
     8401 gctgaaccac cttgccctgc ggcacggctg acagcagttc gggtgagggg cccagcacct
     8461 ttatactcat gttgtctatg tcaaccagtt gcaactccct gttggagtct tccgccttga
     8521 tgacaatatc ataaggctga tgccttacca tattatggca tggcgtccag ctgagccgtg
     8581 ccgtagtatg gccggcgacg ctggagagtg tggtaaactc agccggacag tcgggggagg
     8641 taaagatgcc tgaggttgcc atgagagtga ttgaatcaag gtcaacatcg gtggcatgta
     8701 tatcaagtga aacctggcgg cctgcttcaa cgcacaggtc agtaagctcc gatgttacag
     8761 gagggttgtt gtccgtctcg taaacctcaa tctgcatgtc acgaaccacc acacctatct
     8821 tgatacctgc ccgccattcc tgtatctcca tggcaacatt gtatattcct attgcagtgg
     8881 gtgcatccca gaccagatca cctgagatgg aatcaacata gaattttctt gaggcgggag
     8941 gcagcgtgta gttctgaatg ggtttgccat cctcccttgt acagacggtg agcttgtatg
     9001 agaggctgtc gccgtcaggg tcaaaagctg cagggttgtg aatgaatacc tgccccaggg
     9061 cagccttgtc atatggcgga ttgagcagca ggggagtact gttcctgcca atgcctgtgt
     9121 tgacgatcag tatggtggag atggagaaaa caacatttac cgaatttgga atattttcca
     9181 ctcccagatt ccggttcggg tcctgcacca caatcctgta aaccccgggc ccgggatagg
     9241 tatgctctgc cacgtagaca ttcttcttgt agtcattcgg cagaatcgtc tccgagatcc
     9301 ttgccacgtt ggtgattgag ttatcacccc actccacgtc aagcgacggt ctgtctgcct
     9361 tgctcagtgt atatgtgaat gtggttacag tcacctcgaa ggtgaggtcc gatatctgcc
     9421 tgtatgtaat ctcccctgcc ctgttgtgtg tggctgtggc gctgaacgtc acagccagca
     9481 acaatgccag tgcataaaaa attcgccttt ttatcataat aaaattctga tttctaaggt
     9541 gtgacagaac ccacatgtct gaaattcaca taaacaggtg tctgtctgtt tgatacatgt
     9601 gggtttcatc tgactctgtt tcatttcaca agataactct tcaacgtatc ggttacggtg
     9661 aacctgtagc cctcctccat gccttcatag ccaaagagga gcatgttggc ctgatcttca
     9721 taaagccggg tcatgatgcg gttcatcacg gtaacatcct tcactccttt cctgcccttt
     9781 gccctcaggg tgaagcggtg ttccagacca tcaacgccgg ctgatatcag ttccatgctc
     9841 acgggtttcc catcctccat tatcatgacc ctgtcagtaa gatagaggaa atacctctcc
     9901 tccgtgctgc ctgttatttc gctgccgtcg ctcatgccaa ggttcatgtc agcctcaaga
     9961 tcatcactcc agaccttgac aaaaactgac cagacccttt tcacgccgtc ataatctata
    10021 cctgtaagtg aaacatgcac aggatggatc aggagcagga gtgtgagcgt tattgtcttc
    10081 atgccggcac ggaaataatt gcagcaaaaa tcaagccatc agctcttgtc tggtgacccc
    10141 tgccctctcc atgagatagc caactgtgcc cacctcgatg aaggagaagc cggtacctct
    10201 ggagaagttt cctgccagcg ccacaactat gtcgctctcc ttcatgctga aattcttcag
    10261 gagctccctt accgagacct ggatgaactc atcaactgac attttctttt caatgaatat
    10321 ggggattact ccatatgaca gcatcagttc cctcatggtc cggtgtgagt aacagtgcgc
    10381 gatgaccggg ttaatgcccc tgaaagcagc catgttcctg atggcggttc cattgatggt
    10441 atcagccagg atggctctgg cttttattct tacagaggtt ttcacagcca cctttgccag
    10501 gtatgctgaa accggcccgc tcagggtacc ggctggcatc tcgatgtact gatccttctc
    10561 cttctcagcc tcggaggcaa tgcgtgtcat ggttctgacc gcctctacgg ggtactttcc
    10621 cgcagcagtt tcaccgctca gcatcagggc atcagtctgg ctgtagacag cgttggccac
    10681 atcactcacc tctgcccttg tgggcctggg attctgaatc attgagtgca gcatctgcgt
    10741 ggctactata acaggcttgc gtttctcaat gcacctggca atgatgttac gctgaatgcc
    10801 ggggatacgc tcaaagggaa cctcgatggc gagatcaccc cttgcaacca ttatcccgta
    10861 agagtgatcc atgatctcct cgaggttatc caccccctgc tgattctcta tcttggagat
    10921 tatctttatt gcacttgacc gctgatcaag cagatgctga accgcaagca catcatcctt
    10981 tgacctgaca aaggaatgag ctatgaattc aatgtcaagc tcaaccgcca ggtcaatgaa
    11041 cccgaggtca cgttcactga cagagggcag attaatcctt acctggggaa tgttgacagt
    11101 cttgcgtgaa cccagaacac cgtcattttc tatctttcca atcagagcat ctccttctcg
    11161 gccgataatc tttatctcta tctcaccgtc atcaataagt ataatggctc cctccggaac
    11221 atactccgcg atatcagggt agttgacatt gatgacctca cgggtggtgc cggccgccga
    11281 atcgccggca accctgactg tgtcgccctt gttgaatgtc aggggttcgc tgcataaggt
    11341 tgttcttatc tcaggtccct tggtgtcaag gagtatggcg atatggtcag atatgctgcg
    11401 cacgttcgcc acgatcttcc ttacaccgtc ggcatcaaga tgggcagaat tgacccggac
    11461 aacatccatt ccggcctcat ggagcctgcg caggaaagcg ggttcacagt tcctgtcgga
    11521 aatggttgca actatcttcg ttctcttctt cagcaggttt cttgccatag tcagcttatt
    11581 ttttcagaat ttcgatgagg gcctctgccg caagcctgta gccgtctgtt cccagtccca
    11641 taatggtacc cctgcagtac cgccccgtga ggcttgaatg ccggaactcc tccctggtca
    11701 ggaggtttga aatatgtacc tctattaccg gtatggtgat tgcggcgata gcatcggcta
    11761 tagccacgga tgtgtgagta tagcctccgg gattgatgat gattccgtcg gagctgcttc
    11821 cggcttcctg tatcctgttc acaagttctc cttcgatatt cgactgcgag tacctgaatg
    11881 acacctgcgg gaaacgctcc ttaagccggc caagaacggc ttcaaatccc ccggttccgt
    11941 aaatagccgg ttctctcttt cccaggaggt tgagatttgg cccgttgata atttctattg
    12001 tcattttttc tgctcctgtg acggctctgt atgccggtac aaaaataaag aaatgaccgg
    12061 atttaagacg gttgaaagat ataactttgg gagcagcaac atgagcctat gacaatatcg
    12121 tggacagatg ccataagggg tttcggcagg tatctcagga ttgagcgttc gctttcgccg
    12181 aacagcgttg ctgcatacct ggctgatgta cgacggttgc atgactgggt ggagtcacac
    12241 aggccgggaa gcgggccgtc agagctcacc ccgggtgatc tgagtgattt tgtggctttt
    12301 attgcagcca ggaacgatgc tgtgcggaca caggcaaggg tcatctcggg aatcaggtca
    12361 ttctacgggt atctgctcgt tgaaaacctc gtcaccgatg atccgttaat gctgccggag
    12421 ctgccaaaga caggcatgaa acttccggag gtactcactg ttgatgagat agaccggatg
    12481 gttgcatcca tagacctgag ccagccggca ggacacagga acagggcaat tattgaaacc
    12541 ctgtacggat gcgggctgag ggtatcagaa ctggttaaca tgcgcctcac tgacatccat
    12601 cgtggtgaag gcttcgttac cgtcaccggg aagggcaaca agcaaaggtt agtgccaata
    12661 ggcagtgtaa cgctaaagga gatagacaat tacatggaac gccggatgat gatgccggca
    12721 ataattgaca ccaacatcct ctttctgaac cggcggggca ggagg
//
LOCUS       DAOPBK010000070        12625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_4955_length_12625_cov_5.264360, whole genome shotgun sequence.
ACCESSION   DAOPBK010000070 DAOPBK010000000
VERSION     DAOPBK010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12625
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4955_length_12625_cov_5.264360"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..1514)
                     /locus_tag="PLA17_05630"
     CDS             complement(321..1514)
                     /locus_tag="PLA17_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPK84929.1"
                     /translation="MTTRRSFIRQSGLTAGGLAAATALGASAFPAGEGSPRKQEGLKG
                     LKISKMFAEPLSIEMDPFPIALGVMTHLDNAFVTIVLENGIEGYGEAAPIMTISGENQ
                     QTILGTLNSCREFIVGQDINNYRSIAYTLKSAFWAQSAARCAIEMALLDAYTKSLKIP
                     FYRFLGGTDNRIETDFTIAIVPAEQAKQEAIQLAAEGFRVIKTKVGVNLKEDVERVLA
                     IRDGAPDCGILIDANQGYSPKTALRFINEVVNHGIYPVMFEQPVHKNDLAGMKHVRDN
                     TEILVGADESVFTRADAINIVRTGCADAINIKLMKSCIIESLDIAAVARSANLKLMIG
                     CMVETNLALGCAVHFAAGVGSFEFIDLDPSFEASECPVSGGPVYRAPLWTIGPEPGLG
                     FGKRQ"
     gene            complement(1598..3337)
                     /locus_tag="PLA17_05635"
     CDS             complement(1598..3337)
                     /locus_tag="PLA17_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPK84930.1"
                     /translation="MIFSKFNLLLGTVAALLLAGCGDNALKKARVEAPPATDAILVNQ
                     VGYYPDATKIALLRADADNFELIDASTGRCVFGAESGEPLYWELSGDTVRAADFSAFT
                     TPGTYRICLEGRDICSAPFRIGDDVYGEITRGAVRSYYLNRTAIEITEEFGGKWARPA
                     GHPDTAVIVHTSAASAGRPAGTLISSPGGWYDAGDFNKYVVNSSITNYTLLLFYQMFP
                     DYCRSLDLNIPESGNDIPDLVDELLWNLRWYLTMQDPADGGVYHKLTNLAFNAFMMPH
                     QATEPRYVVMKSTAATLDFAAVTAMAYRVFANDPSEELRALAATCLEASDKAMNWAAA
                     HPDVIYRQPDDVSTGAYGDDNLSDELFWAKAETALAHGKAPGEGFIEGVRAVTPTWNQ
                     SETLGLISMALSSDDSFAPLREAAASLLVAYADELAVKSEKCPYRISLDWFAWGSTSD
                     VANQAMIKIVAMKITGSDRYLPSLQADLNWLLGTNPTGYCFVTGFGTLSPMYIHHRAS
                     GADGVPEPVPGFLVGGPNTVVMNDCQPPVERSIFPAKSYSDVECSYSTNEIAINWNAP
                     LVFLLGAIDSMKP"
     gene            complement(3342..5774)
                     /locus_tag="PLA17_05640"
                     /pseudo
     CDS             complement(3342..5774)
                     /locus_tag="PLA17_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843139.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            complement(5851..7725)
                     /locus_tag="PLA17_05645"
     CDS             complement(5851..7725)
                     /locus_tag="PLA17_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HPK84931.1"
                     /translation="MNLHIIDLGIVILYLLVTIFIGNWVSKRASANIKSYFLGGNTLP
                     WYFLGISNASGMFDIAGTMLLVYWLAVYGLKSIWIPWLWPVFNQIVLMVFLSAWLRRS
                     NVMTGAEWIKTRFGDGRASNLSHIIVVIFAIVSVIGFLSYGFKGIGKFVAAFLPPLVS
                     NPEYLATHPQVNVNLYALILMAITTFYVVKGGMFSVVITEVIQYGILTVASIAVGIIA
                     ISQISPDALNNVIPEGWKTMWFGRTTGLDWTTISSTASPKLHSFNDWIKTDGYELFGL
                     FFVMMLFKGILVSAAGPAPNYDMQRILATKNPRDASRMSSLVSVVLNPTRYFMVAGLT
                     IIALVNFDRLYTGSLTNPDFESILPEVLASYVPVGLLGFLLAGLIAAFMSNFAATVNA
                     APAYLVNDIYKRYVNPHASEKTYVRMSYAASLLIVVIGIAVGFLVTSINDVVLWITAA
                     LWGGYTAPNFLKWYWWRFNGYGYFWGMLSGIAAALLLPALNLELLQNWPLTENFSMNA
                     FPLIFLISLAGSILGSLLTEKEDDETLKRFYRQVRPWGFWKPVEKMVLAEDPSFTPNR
                     DFWRDMFNIVVGIIWQLSLMAFPVFLVIREWKQFYVAVTVMIVTTVILKFTWWNKLKD
                     "
     gene            complement(7750..8496)
                     /locus_tag="PLA17_05650"
     CDS             complement(7750..8496)
                     /locus_tag="PLA17_05650"
                     /inference="COORDINATES: protein motif:HMM:NF040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HPK84932.1"
                     /translation="MISHKIVPNISVDCVVFGFDFEKLNVLLVKREMIDEKSGKALIS
                     DHTLAGYHIFDDEDLDSAAARILKGLTGLDNIFLEQFGAFGQLDRVSSPKDKMWLEYI
                     NQGFADRIVTVGYLALIDNTKVTLTLKDRDVSWFPVSMIAEMEMAFDHKFLFYRALEA
                     LQRKVKVEPIAFELLPDYFTLSQLQKLYEALLGTSFDKRNFRKKVAQMHYVIPLKKKQ
                     QGVPHKPAQFYLFSREVYEKTRKGRISFII"
     gene            8919..12026
                     /locus_tag="PLA17_05655"
     CDS             8919..12026
                     /locus_tag="PLA17_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK84933.1"
                     /translation="MSTTMLFAQQRTVTGRVVDDSNAALPGASVVVKGTTIGTATDLN
                     GNFTLQVPSDATTLVITFVGMDTKEVPVTEGPINVTMTMSAEEVDAVVVIGYGTIRRS
                     EVTSAIASVKTEEIKDLVVTGVDQALQGKVAGLQVMNNSGQPGGGVSVRVRGITSVNS
                     NDPLIVIDGVPFTDNNVENTGYDGLGGSDGQTRNSFLATLNPNDIESIDVLKDASAQA
                     IYGSQAANGVILITTKKGKAGDGKITYDFSYGLQQVSRTLDVMNLREFAEYQNSVIAE
                     LGSGYTPTEEFADPSLLGEGTDWQDAIFRQGYTMDHQLGISGGKDNTSYYFSAGYFDQ
                     KGILIGSDFKRYSTRFNLDSQVKKWLRAGVSSNITRAIQNVTLADAAESTIWWATLQN
                     PLVPVRNLDGTWAGNYTVGGYSYTADNPVATSSSRGNKGVNSQIFGNIYADVIFLEGL
                     SLRNEFSYQIGLQNNMAFQYEANIGTRSLQAQLYDSRSNSYYYAVRNYLNYDKSLGKH
                     RLSFTGGHEAQYSYWETMGGKKVDLQNNILDMNAGGTDKLTWDLTGGKGDWAMESYFV
                     RGNYTFDNRYSLSLSYRGDKSSNFGPNKKWGFFPGASVGWTVTNEKFAANWTNVMNYM
                     KIRLGAGAVGNQNLPGGAPHPPYTSNVNFWPGPVGFGQVGTASTNFIAGIANPDLGWE
                     SVITYNGGIDFGFIRGRIDLTVDLYKKVTSNMLLFSTGPALLGIGDAWNDLKAPIGNV
                     GQMTNTGIDISLVTRNISKPDFSWTTSFIFSHYKNVLDKLINESSSIDGKLYYDNYLI
                     THTVPGYSVGTFWGLVTDGLFRTEADLASSYPQFGLAVSQNETWLGDVRFKDINKDQV
                     IDSKDLTFIGSPIPDFTFGLTNSFRYRNIDLSIFLQGSYGAEIFNFMKWQLERMNNVY
                     YNQSTAVMDRYTETNKDGALPRFTYSNTNNTAMSDRYVEDGSYMRVQNITLGYRLPKH
                     VINRVNMNNLRLFVTVQNLHTFTKYTGYDPEVGSYNNSIRLMNVDAGHYPNPRTYMGG
                     VSVEF"
     gene            12046..>12625
                     /locus_tag="PLA17_05660"
     CDS             12046..>12625
                     /locus_tag="PLA17_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84934.1"
                     /translation="MKNRFIYFILILAATLASCSDSFTDRPSLSGATVSNYYNTADEV
                     RAATSTIYSGLAWSGYEARALDCIGEILSGNEQTYNGDDTPFQLITVSATSVRLSDAW
                     KAFYKINGWTSSLIATFEQKIADGGDAAILEPAIAECHFLRGTVYFYIARIWGHAPIV
                     TNPGAIALTGDFNIPRYYQNDVLRFALEELQLA"
BASE COUNT         3133 a         3293 c         3188 g         3011 t
ORIGIN      
        1 gatgtgcagt atcacggagc tgggacggca gaggtttgcc gcgtatgtgg cagcgctcag
       61 ggattatctc ggatctgtgg accagaagat ttgaagatct cagaatcaga gggtctgagg
      121 gtttttgcct cttttgtcaa ctccctcgcg atcgtgcgtt tgtgaagtat tgcaatagta
      181 tcagagtgcg gcgaaggtcc cgaccggaag gttgggacct tcgtaccgtc accatacttt
      241 aaccgctact gctgcctgcc aacagcagat ttcgctactg cctgcctgct gcctgtattg
      301 cccgccaaca agtgccttca ctactgcctc ttaccgaatc ccagtcccgg ttccgggcct
      361 atggtccaga ggggagccct gtaaaccggg cctccgctga ccgggcactc cgatgcctca
      421 aatgacggat cgaggtcaat gaattcaaaa ctcccgacac cggcagcaaa atgaactgca
      481 caccccagcg ccaggttggt ctcaaccatg cagccgatca tgagcttgag gttggcgctg
      541 cgtgctacgg cagctatatc aagcgactct ataatgcaag acttcataag cttgatgttg
      601 atggcatctg cacaacccgt acgtacaatg ttgatggcat cggcacgggt gaagactgac
      661 tcgtcggcac ccacaagaat ctcagtgttg tcacgcacat gcttcatccc ggccaggtca
      721 ttcttgtgaa caggctgctc aaacatcacc ggataaatac catgattcac cacctcattg
      781 atgaagcgaa gcgctgtttt cggcgaataa ccctggttgg catctatcag aatcccgcaa
      841 tcaggtgcgc catcccttat ggccagcacc ctttcaacat cctccttcag gttgacgccc
      901 acctttgtct ttatcaccct gaatccctcg gcagccagct gtatggcctc ctgctttgcc
      961 tgttcggccg gcacgatggc gatggtgaag tcagtctcaa tcctgttgtc tgttccacca
     1021 aggaacctgt agaacgggat cttcaggctc tttgtgtatg catcaagcaa ggccatttca
     1081 atggcacacc ttgcggccga ctgtgcccag aacgcactct tcagtgtgta tgcaattgac
     1141 ctgtagttgt ttatatcctg gcccacgata aattccctgc aactgttcag tgttcccagg
     1201 atagtttgct ggttctcgcc gctgatggtc atgatcggag ccgcttcacc gtagccctcg
     1261 atgccgttct caagaacaat ggtgacaaag gcattgtcaa gatgtgtcat cacgccaagg
     1321 gcgataggaa acgggtccat ctcaatactc aggggttcag caaacatctt gctgatctta
     1381 aggcctttca gcccctcctg tttccgtgga ctgccttctc cggccgggaa tgccgatgca
     1441 cccaatgcag tggcggcggc cagtccgcct gcggtcagac cgctctgcct gataaatgat
     1501 ctccttgtag tcattgctga ttcctttgtt gcactaatct acaaaatcag atcggatatc
     1561 ctgcaggaat caggctttat ttaatgacac aaattcttca gggcttcatg ctgtcaattg
     1621 cgccaagaag gaataccagc ggggcgttcc aattgatggc aatctcattt gtagagtagc
     1681 tgcattccac atccgagtat gactttgcag ggaagattga cctttcaaca ggcggctggc
     1741 agtcgttcat cacaacggta ttcggtccgc ccaccaggaa gccaggtaca ggttcaggta
     1801 ccccgtcagc gcctgaggcg cggtgatgga tatacatggg tgaaagggtt ccgaacccgg
     1861 tcacaaagca atatcctgtc gggttggtac ccagcagcca gttcaggtca gcctgcaggg
     1921 agggcagata gcggtcgctg ccggtaatct tcatggcaac gatcttgatc atggcctggt
     1981 ttgcgacatc ggaggtgctt ccccatgcaa accagtcaag gcttatgcgg taagggcatt
     2041 tttccgattt cacggcaagc tcgtcagcat aggcgacaag cagcgaggca gcagcctctc
     2101 tcaacggtgc gaaggagtca tcgctgctca gcgccattga gatcagcccg agtgtctccg
     2161 actggttcca tgtgggtgtc accgccctga cgccttcaat gaatccttcg ccgggagctt
     2221 taccatgtgc cagtgcagtc tcggcctttg cccagaaaag ctcatcggag aggttgtcat
     2281 caccgtaggc gccggtgctg acatcatcag gctgacggta aatcacatca ggatgagcag
     2341 ccgcccagtt catcgcttta tccgatgcct ccaggcatgt ggcggcaagg gccctgagct
     2401 cctccgacgg gtcattagca aagacccggt aagccattgc cgtaacggct gcaaagtcaa
     2461 gggtggcggc agtgctcttc ataacaacat accttggctc agtagcctga tgaggcatca
     2521 tgaacgcgtt gaaagcaaga ttggtcagct tatggtaaac cccgccgtcg gcaggatcct
     2581 gcattgtgag ataccatctc aggttccaga gcagctcatc aaccaggtct ggtatgtcat
     2641 tgccgctttc gggaatatta aggtcaagtg acctgcaata atccgggaac atctggtaaa
     2701 acagaagcag ggtataattg gtgatgctgc tgttgacaac gtacttgttg aagtcgcccg
     2761 cgtcatacca tcctccgggg cttgaaatga gtgtgcctgc cggcctgccg gccgaagcgg
     2821 ctgaagtgtg aacaatgacc gcagtatcag gatgaccggc aggacgcgcc catttgccac
     2881 cgaattcctc agtgatctcg atagcggtac ggttgaggta gtaagagcgc actgctccac
     2941 gcgtgatctc accgtacaca tcatcaccaa tccggaaggg agcggagcag atatccctgc
     3001 cctccaggca gatcctgtat gtgccgggag tggtaaacgc cgagaaatca gccgcacgga
     3061 cagtatcacc cgagagctcc cagtacagag gctcgccaga ctctgcgcca aacacacatc
     3121 tgcccgtgga ggcatcaatc agctcgaagt tgtcagcgtc agctctcagc agagctatct
     3181 tcgtagcatc cggataatat ccgacctggt tcacaaggat ggcatcggtt gccggaggag
     3241 cctcaactct tgccttttta agagcattgt ccccacaacc cgccaggagg agtgcggcaa
     3301 ccgttccaag caataaattg aatttactga atatcatgat attacattat tattttaacg
     3361 tcattttccg ttccttctgg aaacaggggc agcatgcatg aatcaacctt ttacccgtca
     3421 acggtaatct cccatactcc ccttgatttc cgttccgggt tgactacctc aatattgtaa
     3481 accgcacccc tgaacctgcg ctttaccgta aagccacccc agtcggccgg gatgcaggga
     3541 tcaacaatca gcccgtcata gtccggacgt attcccagaa tatattgtgt taccgcataa
     3601 tagcaccatg cggcagtgcc tgtcagccag ctgtttttcg cctcaccggg gcggaaggcg
     3661 tccttccctg ctatcatctg cgagtaaacg tacggttccg ttttgtgcag gtcacttatc
     3721 tcctccaggt aagcgggtgt tatccgcttg tagtagtcaa atgcccggta accgttgccg
     3781 gtaagggtct ccgcgatgat gatccacggg ttgttgtggc agaaaacgcc ggcgttctcc
     3841 ttgtatcccc cgggatagct ggagatctct cccaggttga gcatgtactt cgtgaaggcg
     3901 gggttattga gcactattcc atactcgcag gcaaggtatc tgttcaccga ggtcagtgcc
     3961 ttcagagccc tgccgtcatc aagaccaatg ccggccataa tgcacattcc ctgtgattct
     4021 ataaagattt ttccttcatc gttctccctg cttccaacct tccttccgta gtagtcatac
     4081 gccctgagaa accattcgcc gtcccatccg tgtgcattga tggctttaac catctcttca
     4141 atctgcctgg ctgcttcatc agccatccgg gtttctcccc tgcggcgggc gatggtaaca
     4201 aaatctgccc cgtagagcac gaacatagcg gcaatgaaga gcgactcagc cactcctccc
     4261 tttttgtttt cagttgtctg gaaactctca tcgggattca tggagaaaca attcaggttg
     4321 aggcagtcgt tccagtcagc acgccctatc agcggcaatc cgtgaggtcc caggttattg
     4381 accacgtgca tgaatgaccg gcgcaggtgc tcaaagaggc ttgccgcatc atcaggatta
     4441 ttgtcgaatg gaacgttctc gtcaagtatt gaccagtcgc ctgtttcctt gaggtatgtt
     4501 gccacgccga atatcagcca cagcgggtca tcattgaagt tactgcctat ctccgcgttg
     4561 ccgcgcctgg tcagtggctg gtactggtga tatgcgctgc cgtcaggcaa ctgggtggag
     4621 gcgatgtcaa taatcctctc gcgcgcctgt ggcgcgatca tctgaacata tcccgccagg
     4681 tcctggttgg agtcgcggaa tcccatcccc cggcctatac cagactcaaa aaaggaagca
     4741 ctgcgcgaaa aattatatgt ggccaagcac tggtacgggt tccagatatt gaccatccgc
     4801 tcaagccgcg gatcagggtg ggtcagggtg atcaccgaaa gcattctttc ccagtattcc
     4861 gtcagtgccg cgaaggcggc atcaaccttt tcaagggtgt tatacctttc tgtcatcgct
     4921 ttcgctctag ccttgtttat gaccccgggg gcagtaaact tctcattgtc tggattttcg
     4981 acatagccca gcacaaagat atattccttc acctcgcccg gttcgaggtc tgcaatgagg
     5041 cagtgtgagg caacgggcga ccagccatgg gcgatactgt tccggggttg tccggtaaag
     5101 accgcgtcag gcctgtcaaa accgttgtag agtcccaaaa acgcttcacg gtcagtatca
     5161 aaaccggcaa cctgccggtt gaccgaatag aaagcataat ggttgcgacg ctcacgatac
     5221 tcggtcttgt gatagatgac cccctcttca acctccacct cgccggtgga gaagttgcgc
     5281 tggaagtttg tctggtcatc aagtgcattc cagaggcacc actccacaaa cgagaaaagg
     5341 cggatgcgct tcggcatcct gccgttgttg cggagggtga tccgttgcac ctcgcaggtg
     5401 tcgcccgggg gaacgaagaa gagcatgtca gcccgcaggc cgttcttttc cgaaccaatc
     5461 cgtgtataac ccataccatg acggcaggag tagtcttcca catctgccct gaccggcttc
     5521 cagccgggag accagactga gccgttgtca ttgatataaa aatatttgcc cccgtcatcc
     5581 gccggcacgt tgttgtacct gtagcgggtg atacggcgca gacgcgcatc acggtaaaaa
     5641 ctgtagcccc cggcactgtt tgagattata gagaagaaat ccttgttgcc aaggtagttg
     5701 atccatggcc agggtgtcag gtaactggtg atgacatact ccttcgcggc atcatcaaaa
     5761 tatccgtatt tcattgtcag gtcactctta gagttttttc attttcaatt ccggcattgc
     5821 cggagactgg caacaggggt catccccgga ttagtcctta agcttattcc accaggtgaa
     5881 tttcaggatc acggtggtaa ctatcatcac cgtcacggcc acatagaact gtttccattc
     5941 ccttatcaca agaaaaacgg ggaatgccat cagggagagc tgccatatta tacccaccac
     6001 aatattgaac atgtcgcgcc agaaatccct gttgggagtg aaggagggat cctcggcaag
     6061 gaccattttc tcgaccggtt tccagaatcc ccatgggcgc acctgcctgt agaatctctt
     6121 cagtgtctca tcgtcctcct tttcggtaag aagtgatccc aggatgcttc cggcaagtga
     6181 gataaggaag atcagcggga aggcattcat gctgaagttc tctgtcagcg gccagttctg
     6241 aagcagttca aggttcagcg ccggcagcag aagggcagca gcgatgcctg agagcatacc
     6301 ccagaaataa ccgtagccgt tgaaccgcca ccagtaccat ttcaggaagt tgggtgcggt
     6361 ataccctccc cagagcgctg cggttatcca gagaacaaca tcattgattg atgtgacaag
     6421 gaagcctact gctattccga taaccactat cagcaatgag gcagcgtagc tcatgcgcac
     6481 ataggttttc tcgctggcat gggggttgac gtaccgcttg tagatatcat tcacaaggta
     6541 tgccggggcg gcgttcactg tagctgcgaa gttgctcatg aaggcggcaa taaggcctgc
     6601 aagcaggaac ccgagaagac ccaccgggac gtatgatgcc agcacctcag ggagaatgct
     6661 ctcaaagtcg gggttggtga gtgaccccgt atagagcctg tcaaagttca caagggcgat
     6721 gatggtgagg cctgccacca tgaagtaacg ggtgggattg aggactacgc ttaccagtga
     6781 gctcatcctg gatgcatccc tggggttctt ggttgccagg attctttgca tgtcatagtt
     6841 tggcgccggg cctgcagcag agacaagtat ccccttgaag agcatcatga caaagaagag
     6901 tccgaagagc tcgtatccgt cagtctttat ccagtcattg aatgagtgga gcttcggcga
     6961 agccgtagag gagatggtag tccagtcaag tcccgttgtg cggccaaacc acattgtctt
     7021 ccatccctcg gggatgacat tgttgagcgc atcgggagag atctggctga tggcaataat
     7081 tccaaccgct attgatgcta cggtcagtat tccgtactgt atcacttcgg tgataaccac
     7141 gctgaacata cctcccttga ccacgtagaa ggtggtaatg gccatgagga tgagtgcata
     7201 gaggttgacg ttcacctgcg ggtgagtggc caggtactca gggtttgaaa ccagtggcgg
     7261 gaggaatgct gccacgaact tgcctatccc cttgaagccg tatgagagaa aacctattac
     7321 gctgacgatg gcaaatatca ctactattat gtgtgacagg tttgatgctc tgccatcgcc
     7381 gaagcgtgtt ttgatccatt cggctccggt cattacattt gatcgcctga gccaggctga
     7441 gaggaacacc ataaggacga tctggttgaa aaccggccat aaccagggta tccagatgct
     7501 cttgaggcca tagaccgcga gccagtacac aagaagcatt gttccggcta tgtcaaacat
     7561 gccagatgca ttggagatac ccaggaaata ccatggaagg gtgttgccac ccagaaaata
     7621 cgactttatg tttgctgatg ccctctttga gacccagttc ccgataaata tggtgaccag
     7681 gaggtacaga atcactatac ccaggtcaat gatatgcaag ttcatggcgg tggttagttc
     7741 ggtttattgt cagataatga atgaaatgcg tccttttctg gttttttcgt acacctctct
     7801 gctgaagagg taaaactggg ccggtttgtg cggcactccc tgctgtttct tctttaacgg
     7861 gatgacgtag tgcatctgtg ccaccttttt cctgaagttt ctcttgtcaa atgatgtacc
     7921 cagcagtgcc tcgtacagtt tctgaagctg tgacagggtg aagtaatcag gcagcagctc
     7981 gaaggcgatt ggctcaacct tcaccttgcg ctgaagggcc tcaagcgctc tgtaaaaaag
     8041 gaatttatgg tcaaaagcca tctccatctc tgctatcatg gagacaggaa accaggagac
     8101 atcacggtct ttcagggtga gtgtcacctt ggtgttgtca atcagcgcca ggtaacctac
     8161 cgtaacaata cggtcagcaa atccctggtt gatatattcg agccacatct tgtccttggg
     8221 cgaggagaca cggtcaagct gaccgaacgc tccgaactgc tcaagaaaga tattgtcaag
     8281 cccggtcagc cctttcagta tcctggcggc agcgctgtca agatcctcgt catcgaagat
     8341 gtggtagcct gccagggtat ggtcactgat aagagccttg ccgctctttt catcgatcat
     8401 ctctcgtttg acaagcagga cattcagttt ttcaaagtcg aaaccgaaca ccacacagtc
     8461 gactgaaatg ttgggaacaa ttttatggga tatcatttta ttatatcggt gatgtcataa
     8521 aatgacttag tgtacaatgt aagattccaa agatatttca aaattctagt ataatcaata
     8581 ggaatgaaaa aaaagagaat acgcaaatac cgggaaaatt aatctgcgca tcattattaa
     8641 taatgtataa gatagcattt caaaaatgtt gctgcagcca acaaaaccgt ttacctgaat
     8701 gccgctgtat gaataattca tatctttgtg tataatatac accaagaaat gcaaaatttg
     8761 ccttttcaga tgcaggacta atcaaatatt tacaacaaag agacaaaata gtatcagtgc
     8821 tgcgctgaca agaagccgta actttaaaaa cctaaaactg tacagctatg caaaacaaac
     8881 tcttaagatt aacatttctt atttgtctgg cagctgtaat gtcgacaact atgcttttcg
     8941 cgcagcagag aactgtcacc ggaagggtag ttgatgacag caatgctgca ttgcccggag
     9001 cctcggtagt ggtgaagggc acgacaatag ggactgctac tgatctcaac gggaatttta
     9061 ccttgcaggt gccatctgat gccaccaccc ttgtgatcac attcgtagga atggatacaa
     9121 aggaagtgcc ggtcacagaa ggtcctatta acgttacaat gacgatgtct gccgaagaag
     9181 ttgacgctgt tgttgtgatc ggttacggta cgatcaggag atcagaagtg acatctgcca
     9241 ttgcctccgt gaaaacagag gagataaagg accttgttgt taccggtgtt gatcaggctc
     9301 ttcagggcaa ggtggccggt cttcaggtca tgaacaacag tggtcagccg ggcggaggcg
     9361 tctcggtaag ggtaaggggt atcacatccg tcaacagcaa tgacccgctc atagtaattg
     9421 atggtgtccc gtttactgac aacaatgttg agaatacggg atatgacggt ctgggaggtt
     9481 ctgacggcca gacccggaac agcttccttg caacccttaa ccccaatgat atcgaatcaa
     9541 ttgatgtgct gaaggatgct tcggcacagg ctatctacgg gtcgcaggca gccaacggcg
     9601 ttatcctgat aaccaccaag aagggaaaag ccggtgacgg aaagatcacc tatgattttt
     9661 catatggcct ccagcaggtg agcaggacgc ttgacgtaat gaacctgaga gagtttgccg
     9721 agtaccagaa cagtgttatt gccgagcttg gttccggata cacacccacc gaggagtttg
     9781 cagacccctc cctgcttggt gaaggaactg actggcagga tgcaatattc cgccagggat
     9841 acactatgga tcaccagctg ggcatctcag gagggaagga caatacaagc tattacttct
     9901 ctgccggcta ctttgaccag aagggaattc tgataggatc agacttcaag cgctactcaa
     9961 cccgtttcaa ccttgacagc caggtcaaga aatggctgag ggcaggggtc agctcgaaca
    10021 tcaccagggc tatccagaac gttaccctgg cagatgctgc tgagagtacc atatggtggg
    10081 ccacacttca aaaccccctt gtcccggtca ggaaccttga cggtacatgg gccggcaact
    10141 ataccgtggg aggctattcc tacacagctg acaaccccgt ggcaacaagc agcagccgtg
    10201 gcaacaaggg tgtaaacagt cagatattcg gaaatattta tgctgacgtg attttccttg
    10261 agggattgtc tctcaggaac gaattctcat accagatcgg gcttcagaac aacatggcat
    10321 tccagtatga ggcaaatatt ggaaccaggt cactgcaggc acagctctat gacagccgca
    10381 gcaactctta ttactatgct gtgaggaact acctgaacta tgacaagagc cttgggaagc
    10441 acagactctc cttcaccggc ggccatgaag cacagtactc ctactgggag accatgggag
    10501 gaaagaaggt tgacctgcag aacaatatcc tcgatatgaa cgcaggaggt actgacaagc
    10561 tgacatggga cctcaccggc ggcaagggcg actgggccat ggagtcatat ttcgtcaggg
    10621 gcaactatac ctttgataat cgttactcac tttctctcag ctacaggggt gacaagtcat
    10681 ccaacttcgg cccgaacaaa aagtggggct tcttcccggg tgcatctgta ggatggacgg
    10741 tcacaaacga gaagttcgcc gccaactgga ccaatgtgat gaactacatg aagataagac
    10801 tgggcgccgg tgccgtgggt aaccagaacc tgcccggcgg agctccacac ccgccttaca
    10861 cctcaaatgt caacttctgg ccaggcccgg tgggattcgg acaggtgggc accgcatcaa
    10921 ccaacttcat tgccggtatc gccaatcctg acctcggatg ggaatcagta ataacgtata
    10981 acggagggat agacttcggt ttcatcaggg gcaggataga cctgaccgtt gacctgtaca
    11041 agaaggtcac atccaacatg cttctattca gcacgggccc tgccctgctg ggtataggcg
    11101 atgcatggaa cgacctcaag gcacccatag gaaacgtcgg tcagatgacc aacaccggca
    11161 ttgacatcag cctggtgacg cgtaacatct cgaagcctga tttctcatgg accacctcct
    11221 ttatcttctc gcactacaaa aacgttcttg acaaactgat caatgagtca tcatcaatag
    11281 acggcaagct ctattatgac aactacctga tcacccatac cgttcccggc tattcggtcg
    11341 gaaccttctg ggggctggtg actgacgggc tcttccgcac agaagctgac cttgccagct
    11401 cctacccgca gttcgggttg gcagtttctc agaatgagac atggctgggt gatgtcagat
    11461 tcaaggacat taataaagac caggtgatag attcaaagga ccttactttc atcgggagtc
    11521 cgatacctga cttcaccttc ggcctgacca acagcttccg ctacaggaat attgatctct
    11581 ccatcttcct tcagggaagc tacggagccg agatattcaa cttcatgaaa tggcagctcg
    11641 agaggatgaa caacgtctat tacaaccagt cgactgctgt catggaccgt tacactgaaa
    11701 ccaacaagga cggagctctt cccaggttca cctattccaa caccaacaat acggcaatgt
    11761 ctgatcgtta tgttgaagac ggctcctaca tgcgtgtcca gaatatcacc ctcggataca
    11821 ggctgccaaa gcatgtgatc aacagggtga acatgaacaa cctgagactg ttcgttacgg
    11881 tccagaacct ccataccttc accaaatata caggctatga tcctgaagtt ggctcttaca
    11941 acaacagcat caggctgatg aacgttgatg caggtcacta ccccaacccg cgcacataca
    12001 tgggtggtgt aagtgttgag ttttaatgac tttaactaaa tgatcatgaa aaacagattt
    12061 atatatttca ttctgatact ggcagcaaca cttgcatcat gcagtgattc attcactgac
    12121 cgcccgtcgc tttcaggggc tacagtgagt aactactaca acactgccga tgaggtgagg
    12181 gctgccacga gtacaatcta ttcaggactg gcatggtcag gatatgaagc ccgtgccctc
    12241 gactgcattg gtgaaatcct ttcgggaaac gagcaaacct ataacggaga tgacaccccg
    12301 tttcagctga taactgtctc ggccacctcg gtgcgtctct ctgacgcatg gaaagccttt
    12361 tacaagatca acggatggac aagctcactg attgccactt ttgaacagaa gatagccgat
    12421 ggcggtgatg cagctattct tgaacctgca atagccgagt gccatttcct cagaggaacg
    12481 gtctattttt acatcgcccg catatggggt catgctccca tcgtaactaa cccgggcgcc
    12541 attgcactga caggtgactt caacatcccc cgctattatc agaacgatgt tctgcgtttc
    12601 gccctcgagg agttgcagct tgctg
//
LOCUS       DAOPBK010000071        12203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5147_length_12203_cov_5.056635, whole genome shotgun sequence.
ACCESSION   DAOPBK010000071 DAOPBK010000000
VERSION     DAOPBK010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12203
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5147_length_12203_cov_5.056635"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1192)
                     /locus_tag="PLA17_05665"
     CDS             complement(17..1192)
                     /locus_tag="PLA17_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HPK84935.1"
                     /translation="MKYNFEEAPDRVNTNCLKWDKREEVFGKADVIPMWIADMDFRTP
                     PFIIEALRERLNHEILGYSYRPDSYFKAFMNWVSTLHGWDIRQEWIEFSPGVVPALNV
                     CTLAYTSPNDEIIIQPPVYPPFYGAANDHGRRLVFNQLLETEKGYQMDFEGLRRIITP
                     ATRMLILSNPHNPVGRVWSRDELNELAEICHEKGIIILSDEIHSDLLLPGMKHVPLAS
                     VSEKAAAVTVTCMAPSKTFNLAGLSTSSMIISDPVLMEKFRKTLTGLHIHLGNIFGNV
                     ASEAAYTHGREWLEQLMRYVGENIDLVVSFCRERMPVIRPVRPEATYMIWLDCRAMGM
                     NGQELNRFFVESAGVGMNEGSRFGPGGEGFMRMNLACPGATVMRALEQIESALKSAR"
     gene            1383..2204
                     /locus_tag="PLA17_05670"
     CDS             1383..2204
                     /locus_tag="PLA17_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84936.1"
                     /translation="MKKSFTKLLVAVLILTVAGVSACKNRGESRKQKEINAVELGDLE
                     QKIEENVYPLPTSAEVIKRLTDMDLGYILGAINPPANARNYVESYNRSVNLGVYGADL
                     SYVTLYNMQQEVIDYLAAIRTLALEQNLSKIYDESLYDRIKASFDDRDTLVTILTDAF
                     DRTYSYMLDAGQANLSVLMLGGAWVEGIYLTLLVSESGAHVSGFETALLSQRKAFEEF
                     DELAAAYNSDPLVSRLLTALQPIRDLYAGLGEGLTLEDIERLKQTVTTVRAELIK"
     gene            2260..5382
                     /locus_tag="PLA17_05675"
     CDS             2260..5382
                     /locus_tag="PLA17_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK84937.1"
                     /translation="MRESVLHALIHIFAIISTVNPSGVTARGKKILRSYLRRYLNQEL
                     EEEYFRLYEDNLHFYEHELKAVVEGELSDEDSLITFQITNICRQIRKGLFIEERMIVF
                     LQLLEFVFEDGTVSEQENSIIDIVARTFNISKEERTNAHAFMLGMKFDKITPESLLAI
                     EGESNIGEPSSRYVNYHEWHHMVSHGMRGTIYVLFVRSTGHLLFMYDGKMHLHFRGRN
                     VVPMRPYLLDPGVNIRGHGMAPLYYTAIFRKFIRANFAEDILFEGQNIKYHFKGSENG
                     IQPMSFSVKSGNLVGIMGGSGVGKSTLLNILNGKLKPETGAVYINGYNLHDKPDELNG
                     LIGYVPQDDLLIEELTVYQNLYYNARLTFGDYDETRLNQVVVEMLKELDLYEFRDLLV
                     GDPLNKKISGGQRKRLNIGLELMREPAVLFADEPISGLASHDSGNVMELLKNQAVNGK
                     LVFVIIHQPSSDILKMFDRLWVLDRGGYMIYDGDPVEAIVYFKTETSQANAAESECPS
                     CGNVETDQILQIVEAKVVDSDGRRGQERQVSPVEWYQRYRQKMEPALSGTPEMKPLPA
                     SNFKLQKRNKQVATFFRRNLARKYADKQYMAINLIEPPLLAFILAFVSKYHSGSVYLF
                     SDNKSYPVFLFMSVVVALFLGLTVSAEEIFRDRKVLEREKYLNISRSSYLLSKITFLF
                     GLSAVQTLMYVLIAQHILQVEGMMWQQWLIMFTTSCFGNLVGLNISAGMKTVISIYIL
                     IPLVLVPQLLLGGAMIRFDDLHHSLTKKVYVPVIGDIMTTRWAYEAMAVEQFRSNRYM
                     KPFFETDMMISRYDWQSGYLINQLKRKNNEALYASDKPEYEYACRNSLEKLKTHIGEL
                     SAETGLDPARAMMALNSEPFTREASGIVNECLDSLQKRIRGKFLEQTAVKDSITDALV
                     AAMGRDELVRLKTAVYNNDLADIVLSRTVQNKYHETEERIIQKSDAVLMVPDSRIGRA
                     HFYAPCKMLGNLRIGTLWFNMMVIWLMNIFLFFTLYYNLLKLLLNLMERIKIPGFGSD
                     RLVPPWELIR"
     gene            5364..6167
                     /gene="rsmA"
                     /locus_tag="PLA17_05680"
     CDS             5364..6167
                     /gene="rsmA"
                     /locus_tag="PLA17_05680"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPK84938.1"
                     /translation="MGAYQIEIIMPGPRIRPKKHLGQHFLKDKRIAAAIVNALRADGC
                     DSVLEVGPGMGVLTNLLAERELPAFRVVEIDRESVDYLRQNVTGQVEIIEGDFLELDL
                     HRMGNLIAVIGNFPYNISSQIFFKILDNRDHVTEMVGMVQKEVAERICSEPGSRVYGI
                     LSVLLRAWYDIEYLFTVSEHVFSPPPKVKSAVIRLTRNNVERLGCDEKLFVSVVKRCF
                     NQRRKMLRNPVKAMVNSGAGDMPLLSRRAEQLSVSQFVELTNWVEENRV"
     gene            complement(6245..7492)
                     /locus_tag="PLA17_05685"
     CDS             complement(6245..7492)
                     /locus_tag="PLA17_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK84939.1"
                     /translation="MWFRMFRESFMFAFGSVAANKLRTFLTLFGITIGILSIISVFTV
                     FDWMEKSIKDSIATLGDNTIYVEKWPWTPDANIKWWDMMKWQPPSLDDYEAIVKRSST
                     AEAVCYSIGASEAVKYRGNLASDTYILAATHDFENIRSFEIERGRYFSLSESASGKPV
                     AIIGYEVADQLFGTVQPIGREITIDKYKAQVIGVFKKEGKGGISDNGMDQVVLVPLNF
                     SRHFINFRNPFLNSDIMIRGKEGVSVQELSDEVIMILRAARRIGPGKENTFSINRASQ
                     ITSTLKPVFRGINIAGWIIGGFSILVGGFGIANIMFVSVRERTSIIGIQMALGAKRRW
                     ILQQFLTESVLLSISGGILGLILVFIGTLFVNYFFTLGMHLTAGNILLAVFISAVIGI
                     VAGFAPASSASRMNPVEAIGYSF"
     gene            7652..9178
                     /gene="purH"
                     /locus_tag="PLA17_05690"
     CDS             7652..9178
                     /gene="purH"
                     /locus_tag="PLA17_05690"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPK84940.1"
                     /translation="MMSDIKIKRAFISVYCKDGLEAIVKKLSKLGVAIVSTGGSWDFI
                     TALGVPAVKVEDITTYPSILGGRVKTLHPGVFGGILARRGNDSDMKQVAEYNIPLFDL
                     VIVDLYPFGETVASGASDQEIIEKIDIGGISLIRAGAKNWADVLIISSRNQYDGLMKI
                     LDNGKGVVTSDERYRFALEAFAMTSHYDSAIFNWFNRNGDVKVFSKSITQSHTLRYGE
                     NPHQKGVFFGDPDSSFVQLHGKEISYNNLLDIESAISLIDEFDDLSCAIIKHNNACGV
                     ASRPELKEAWEAALACDPLSAFGGVVIVNREVDEIAAAEIDKIFLEVVIAPSFTPGAV
                     KILSSKKNRIILQRRSSGNDRESFRSMLGGVLWQERDSSTEQASDMRPVTTRQPSEEE
                     YADMVFANKIVKHSRSNAIVLAKSRQLCASGIGQTSRIDALRQAVAKAKTFGFSLEGA
                     VMASDAFFPFADSVTTAHEAGISAVVQPGGSVRDQESIDYCNKTGVAMVFTGIRHFKH
                     "
     gene            9211..10233
                     /locus_tag="PLA17_05695"
     CDS             9211..10233
                     /locus_tag="PLA17_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPK84941.1"
                     /translation="MGFFSFLSQEVAIDLGTANTNIIVNDKLVVDEPSIVAIDRNTGR
                     LIAIGDRARQMHGRTHENIRVIRPLRDGVIADFNAAELMIRGMIRMISSGPKFFSPSL
                     KMVVGIPCGSTEVGIRAVRDSSEHAGGRDVYLIYEPMAAAVGIGLDVEAPEGCMVVDI
                     GGGTTEIAVIALGGIVCNRSIRVAGDGFTSDIQAYMRHQHNIKIGDKTAEDIKIAVGA
                     ALPDIEDPPADFVVRGPNLMTSLPIEIPVSYQEIAYCLDKSLTKIEASILQVLEQTPP
                     ELYSDIVNKGVFLAGGGALLRGLDKRLTDKINIPFRVAEDPLHSVVRGTGVALKNVEK
                     YPFLMR"
     gene            10247..11071
                     /gene="mreC"
                     /locus_tag="PLA17_05700"
     CDS             10247..11071
                     /gene="mreC"
                     /locus_tag="PLA17_05700"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPK84942.1"
                     /translation="MRSLLNFLLRFKTLILFLVLEAVALVMISTSHNYHQTVFYGLSR
                     NISGFVARRVESATYYFRLRSVNEELVRENTLLRRRLEELTARPAEGFTGADDSISGV
                     NYTYMSARVISNSVNKQKNFITLDRGSKHGVEVGMGVASPAGVVGVVVGTSPNYSVVM
                     SLLNIDFRLSTSIARNDYFGSLAWDGINYRYAKLSEIPHHVTVSEGDTIITSGYSAIF
                     PPGLMAGTVTGDQKRGGDFVSMKVMLSVDFKKLTDVYVIGNLTREEWENLEEEVVR"
     gene            11068..11571
                     /gene="mreD"
                     /locus_tag="PLA17_05705"
     CDS             11068..11571
                     /gene="mreD"
                     /locus_tag="PLA17_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPK84943.1"
                     /translation="MNSFLRYTGAFVLLVALQLLIFNNIEFSGYVNPYVYVMFILILP
                     VSIPSWILLLLGFLTGFVIDIFSGTMGVHTFATVLAGFVRPWVLSLNVTAEAAEDDMS
                     PSARRSGLRWFLVYTVTVVLVHHLALFFVELFSFRYFFHTLLRVLLSTAVTTFFILLF
                     DFIRPHR"
     gene            11582..>12203
                     /locus_tag="PLA17_05710"
     CDS             11582..>12203
                     /locus_tag="PLA17_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPK84944.1"
                     /translation="MKDRYAHRKYVIMALVLMAAMILIARLFYIQIVDKTYRVSAANN
                     ALRPVTQYPARGLIYDRNGKLLVYNQAAYDLLVIPVQTSAFDTTRFNEILGITMDDFK
                     TRMKAAISYSRRAPSVFMKMISSETYAILQEELYLFPGFYVQPRTLRKYTRPVAAHLL
                     GYVGEVDNKLIAKDKYYRSGDYIGVSGIEKSYEEHLRGRKGVNIFLV"
BASE COUNT         3042 a         2969 c         3202 g         2990 t
ORIGIN      
        1 tactgactga tgtttcttac cttgcactct ttagcgctga ttctatctgt tcaagggcgc
       61 gcataaccgt tgcccccggg caggccaggt tcatgcgcat gaatccttcg ccccccgggc
      121 cgaagcgcga tccttcattc atgcccacgc cggcgctttc aacaaagaag cggttcagct
      181 cctgcccgtt catgcccatg gcccggcaat caagccatat catataggtg gcttccggtc
      241 gcaccggcct tataacaggc atacgttcac ggcagaagga gacaaccagg tcaatattct
      301 ccccaacata ccgcatcagc tgttcaagcc actcacggcc atgtgtatag gccgcttcag
      361 aggcaacgtt gccgaatatg ttaccaaggt gaatgtgaag tcctgtcagc gttttccgga
      421 atttttccat cagtaccgga tcggaaatga tcatcgagga ggtcgacagt cccgccaggt
      481 tgaacgtttt gcttggcgcc atacaggtta ctgtcactgc agccgccttc tccgagaccg
      541 atgccagcgg cacatgcttc atacccggca acaacaggtc tgaatgaatc tcgtctgaaa
      601 ggatgataat tcccttttca tggcatatct ctgccagctc gttcagctca tccctgctcc
      661 acactcttcc gacagggttg tgcggattag agaggataag catgcgtgtt gccggggtga
      721 ttatcctgcg gaggccctca aagtccatct gatatccctt ctccgtctca agaagctgat
      781 tgaacaccag gcggcgcccg tgatcatttg ccgctccgta gaagggcgga tagaccggtg
      841 gctgaatgat tatctcatcg ttgggggagg tgtatgcaag cgtgcagacg ttgagagccg
      901 gcacaacccc ggggctgaac tcgatccact cctgcctgat atcccatccg tgcagtgttg
      961 aaacccagtt catgaatgct ttgaaataac tgtccggcct gtatgagtat ccgagtattt
     1021 catggttcag cctttcgcgg agcgcctcaa taataaaagg aggtgtcctg aagtccatat
     1081 cggctatcca catcgggatc acgtcggctt tgccgaagac ctcctcacgc ttgtcccact
     1141 tgagacagtt ggtgtttacc cggtctggcg cctcttcgaa gttgtatttc atgagactat
     1201 aaatgctacg ggatgcaaag ttaacagaaa atgataatga actaccggcc tgatgccgct
     1261 gttcaggatg gatgaacacc gggtttgcat tgaccctgaa taattctgag atattattcc
     1321 ggccataaac ttaaaaagtc taattttgcg tagccaaaac acaagtatta aatatcagtc
     1381 atatgaaaaa gagtttcacg aaattactgg tcgctgtatt gatcctgaca gttgccggtg
     1441 tatccgcctg caagaacagg ggtgaatcaa ggaaacagaa ggagatcaac gccgttgaac
     1501 tcggagacct ggaacagaag atagaagaga atgtttatcc gcttcccaca tcggcagagg
     1561 taatcaagag gcttaccgac atggacctcg gttacattct cggtgctatc aatcctcctg
     1621 caaatgcccg caactatgtt gagagttaca atcgctcggt caacctcggc gtctatggcg
     1681 ctgacctcag ctatgtaacg ttgtataaca tgcagcagga agtgattgat tatctggcgg
     1741 ccatccgcac gctggcctta gaacaaaacc tgtcgaagat atatgatgaa tctctctatg
     1801 acaggatcaa ggccagtttt gatgatcgtg acacgcttgt gacaatcctt actgatgcct
     1861 ttgaccgtac ctattcatat atgcttgatg ccggtcaggc caacctctcg gttctcatgc
     1921 tgggcggcgc gtgggtagag ggtatttatc ttaccctgtt agtctccgaa tcaggtgccc
     1981 atgtgtcagg ctttgagaca gccctgctga gccagagaaa ggcgtttgag gagtttgatg
     2041 aactggctgc tgcgtataac agtgaccctc ttgtgtcgag gctcctgacc gccctgcagc
     2101 ccatacgtga tctctacgcc gggctgggcg aaggccttac actggaagac attgaaaggc
     2161 tgaaacagac cgtcaccacg gtcagagcgg aactgattaa gtaataaaaa acagatacct
     2221 gaagaccgca agtaaaaact gcggtctttt tttggggtta tgagggaatc agtattacat
     2281 gcgctgatac atatttttgc cattatctct actgtaaacc cttcaggggt tactgcaagg
     2341 ggcaagaaga tcctgcgcag ttaccttcgg agatatctta accaggagct tgaggaggag
     2401 tatttcagac tctatgaaga caatctccat ttttatgagc atgagcttaa ggctgtggtg
     2461 gagggagaac tctcagacga ggactccctg atcactttcc agatcaccaa tatctgcagg
     2521 cagataagga aggggctctt cattgaggag aggatgatag tcttccttca gttgctcgag
     2581 ttcgtctttg aagacgggac tgtctcagag caggagaact ccattattga cattgttgcg
     2641 cgaaccttta atatctcgaa ggaagagcgg accaatgccc atgccttcat gctcgggatg
     2701 aagtttgaca aaatcactcc tgaaagcctg ctggcaatcg agggcgagag caacatcggt
     2761 gagccctcgt caaggtatgt taactatcat gagtggcacc atatggtctc tcacgggatg
     2821 cgcggcacca tctacgtact cttcgtccgc agcaccggcc atctgctgtt catgtacgat
     2881 ggcaagatgc acctgcattt cagggggcgc aacgttgtgc cgatgagacc atacctcctc
     2941 gatcccgggg tcaatatccg cgggcacggc atggcaccac tctattatac cgccatcttc
     3001 agaaaattca tcagggccaa ctttgctgag gatatcctct ttgaagggca aaacatcaag
     3061 tatcacttca aaggctcgga aaacggtatt cagcccatga gcttcagcgt gaagtcgggc
     3121 aacctggtag gcataatggg tggcagcggt gtaggcaaaa gcaccctgct gaacatcctc
     3181 aacggtaagc tgaaaccgga aaccggagct gtatatatca acggctataa tctgcatgac
     3241 aagcctgacg aacttaacgg cctcataggc tatgtgccgc aggatgacct tctgattgag
     3301 gagctgacag tctaccagaa cctctattac aacgcgcgtc ttaccttcgg cgactatgac
     3361 gagaccaggc tcaaccaggt tgtggttgaa atgcttaagg aactggatct gtatgaattc
     3421 cgtgacctgc tggtgggtga tccgctcaac aagaagatca gcggcgggca gcggaagagg
     3481 cttaacatag gactggaact tatgcgtgag cctgcagttc tttttgccga cgagcctatc
     3541 tcaggacttg catcgcatga ctcggggaat gtgatggagc tgctcaagaa ccaggctgta
     3601 aacggcaagc tggtctttgt gatcattcat cagccgtcgt ctgacatact taagatgttt
     3661 gaccggctct gggtgcttga caggggagga tatatgatct acgacggtga tccggtagaa
     3721 gccatcgtct acttcaagac agagacttca caggccaatg ctgccgagag cgaatgcccc
     3781 tcgtgcggca atgtggagac agatcagatt ctccagatag ttgaagccaa ggtggttgat
     3841 tctgacggga ggagagggca ggagagacag gtgtcaccgg tggagtggta ccagcgctac
     3901 aggcagaaga tggaacccgc cctctccggc actccggaga tgaagccgct gcctgccagt
     3961 aatttcaagc ttcagaaacg gaataaacag gttgccacat ttttcaggag aaaccttgca
     4021 aggaagtatg cagacaagca gtacatggcc atcaacctta ttgagccacc cctgcttgcc
     4081 tttatccttg ccttcgtctc aaagtatcac tctggcagcg tatacctctt ctctgacaac
     4141 aagagctatc cggtcttcct gttcatgagt gtggtggtcg cactcttcct tggtctcacg
     4201 gtcagtgcgg aagagatttt cagggacagg aaggtacttg agcgggaaaa atatctgaac
     4261 ataagccgct caagctatct cctctcgaag atcaccttcc tgttcgggct gtcagcagtg
     4321 cagacactga tgtatgtgct tatagcacag catatcctcc aggttgaagg gatgatgtgg
     4381 cagcaatggc tgataatgtt caccacttca tgctttggca atctggtggg tctgaatatt
     4441 tcagccggca tgaagaccgt cataagcata tacatactca ttccccttgt gctggtaccc
     4501 cagcttctcc tcggcggcgc catgatcagg tttgatgacc tgcaccactc cctgacaaaa
     4561 aaggtgtacg tcccggtgat aggtgatatc atgacaacga ggtgggcgta tgaagccatg
     4621 gctgttgagc agttcaggtc aaacaggtat atgaagccgt tctttgaaac ggatatgatg
     4681 atcagccggt acgactggca atcaggttac ctgatcaacc agttgaagcg caagaacaat
     4741 gaggctctct atgcttcgga taagccggag tatgaatatg cctgcaggaa cagtctggag
     4801 aaactcaaaa cccatatcgg cgagctcagc gctgaaacag ggcttgatcc tgcccgggcc
     4861 atgatggcgc taaacagtga accgttcacc cgcgaggctt ccggcattgt aaatgaatgc
     4921 ctcgattctc ttcagaagag gatacgcggg aaattcctgg aacagacagc tgttaaggac
     4981 agtattacgg atgctcttgt ggcagctatg gggagggatg agctggttcg tctgaaaacg
     5041 gctgtctaca acaatgacct tgctgatatc gtgctgagcc ggacagtgca gaacaaatac
     5101 cacgagactg aagagcggat tatccagaag tcggatgctg tgctgatggt gcctgattcg
     5161 cgcatcggca gggcccattt ctatgcaccc tgtaagatgc ttggtaacct taggataggg
     5221 acactctggt tcaacatgat ggtgatatgg ctcatgaaca tatttctgtt ttttaccctt
     5281 tattacaatc tcctgaagct gttgctgaac ctgatggaga gaattaaaat cccgggcttc
     5341 ggatcagacc gtctggtgcc gccatgggag cttatcagat agaaatcatc atgccaggcc
     5401 cccggataag accaaaaaag cacctcggac agcatttcct caaggataag cggattgcgg
     5461 ctgccattgt aaacgcactg cgtgccgatg gctgtgactc agtgcttgag gtgggacccg
     5521 ggatgggagt gctgacgaac ctgctggcgg agagagagct tcccgccttc agggttgttg
     5581 agattgaccg ggagtcggtt gattatctga ggcaaaatgt gacagggcag gtagagatca
     5641 ttgagggtga tttcctggaa cttgatctgc accggatggg aaacctgatt gctgttatag
     5701 ggaattttcc ctacaacata tcaagccaga tattctttaa aatccttgat aaccgtgatc
     5761 atgtcaccga gatggttggc atggttcaga aggaggttgc ggagcgaatc tgctcagagc
     5821 cggggagtcg tgtctacgga atactcagcg tgcttctgcg tgcatggtat gacattgaat
     5881 acctgttcac cgtcagcgaa catgtttttt cacctcctcc gaaggtcaag tcagccgtga
     5941 tacgcctgac acgcaacaac gtggagcgtc tcgggtgcga tgaaaagctg tttgtcagcg
     6001 ttgtaaagcg ttgtttcaac cagcggcgca agatgttgcg caacccggtg aaagcgatgg
     6061 tgaacagcgg cgccggagat atgccgctgc tctcccggag ggctgagcaa ctatcggtaa
     6121 gccagtttgt ggagctgacc aactgggtgg aagagaacag ggtgtgagtt aacccttgtg
     6181 agaataagtt ccctctcaac ggggcgccgg caacatgtgt tgtcagggtg tgactgaccc
     6241 cttgtcagaa tgagtagcct atagcctcaa cagggttcat cctcgaggca gatgaagcag
     6301 gagcaaagcc cgcaacaatc cctatgacag ccgagataaa cactgcaagc agtatattgc
     6361 ccgcggtaag atgcatcccc agggtaaaga aatagttcac aaaaagagtt cctataaaca
     6421 caagtatcag cccgagtatc ccgccggaga ttgaaagcag cactgattcg gtcaggaatt
     6481 gctgaagtat ccatctcctc tttgccccga gcgccatctg tattcctatg atgctggtac
     6541 gttcacgtac ggatacaaac atgatgtttg caatgccgaa tccgcctaca agtatcgaga
     6601 atcccccgat gatccatccc gctatgttga tgcctctgaa caccggcttg agggtgctag
     6661 taatctggct tgccctgttt atcgagaagg tgttctcctt gcctggtcct atgcgcctgg
     6721 cggcgcgaag gatcattatc acctcatcgg agagctcctg aactgatacc ccctctttcc
     6781 cccttatcat gatatcagag ttgaggaacg gattacggaa gttgatgaag tgacggctga
     6841 agttaagcgg taccagtact acctgatcca tgccgttgtc acttattccg cccttgccct
     6901 ccttcttgaa gactccgatg acctgggctt tgtatttgtc aatggttatc tccctgccta
     6961 ttggctgaac agttccgaaa agctggtcag ccacctcata accgataatt gcaaccggtt
     7021 tgcccgatgc tgactctgac agggagaaat agcggcctct ctctatctcg aatgacctta
     7081 tattctcaaa gtcatgcgtg gcagccagaa tatatgtatc actggccagg ttgccacggt
     7141 attttacggc ctctgatgcc ccgatagagt agcaaactgc ctcagcagtg gaagatcgct
     7201 tcactatggc ttcataatca tccagtgaag ggggctgcca tttcatcata tcccaccact
     7261 taatgttggc gtcaggggtc catggccatt tctcaacata aatggtgttg tcaccaaggg
     7321 tggcgatgct gtccttgatg cttttctcca tccagtcgaa caccgtgaat accgagatga
     7381 tggatagaat cccgatggta atgccgaaga gagtcagaaa tgtacgcagt ttattcgccg
     7441 ccactgaacc gaaggcgaac ataaagctct ccctgaacat tctgaaccac atctccccgt
     7501 ctttaaaatg cgatcaaaga taattattta tgaaacatca acacatcccc gataattcat
     7561 caatccaatt atttgataaa tttgccaatc aaaaaaatta tctattttta tctgattact
     7621 tctgccttgg cgccgttaat ccaagttatt aatgatgagt gacataaaaa taaagagagc
     7681 ctttatttcg gtttattgta aggatggcct tgaagcgatt gtgaaaaaac tgagtaaact
     7741 gggtgttgcc attgtttcaa cgggcggatc atgggatttc attactgctc tgggtgtgcc
     7801 tgcagtgaag gttgaggata taacaaccta tccttccatc ctcggaggac gggtcaaaac
     7861 cctgcatccc ggagtattcg ggggtatcct tgcaaggagg gggaatgatt ctgacatgaa
     7921 gcaggtggcc gaatataaca ttccgctctt tgatctggtg attgtagacc tctatccctt
     7981 cggggagact gtagcttcgg gcgcctcaga ccaggagata attgagaaga tagatatcgg
     8041 cggtatttcc ctgataaggg ccggagcaaa aaactgggct gatgtgctga taatttcatc
     8101 caggaaccag tatgacggcc tgatgaagat tcttgataac ggaaagggcg ttgtcacctc
     8161 agatgagagg taccggtttg ccctggaggc atttgcaatg acttcacatt atgactctgc
     8221 aatattcaac tggttcaaca ggaacggcga tgtcaaggtg ttcagcaaaa gcataacgca
     8281 gtcgcatacc ctgagatacg gcgagaatcc ccaccagaag ggggttttct ttggtgatcc
     8341 tgacagctcc tttgttcagc ttcatggcaa ggagatttca tacaacaatc tgcttgacat
     8401 agagtcagcc atcagtctca ttgatgagtt tgacgatctc tcctgtgcca tcatcaagca
     8461 taacaatgca tgcggagtgg cttcgcgtcc ggagctgaag gaggcatggg aggctgcgct
     8521 ggcctgtgac ccgctgtcgg ctttcggggg tgtggtcata gtgaaccggg aggttgatga
     8581 gatagctgct gcagagatag acaagatatt cctcgaggtg gtgatagccc cctccttcac
     8641 gccaggtgcg gtgaagatcc tcagctcaaa aaagaacagg atcattcttc agcggcgcag
     8701 cagcggaaat gatagggaat cattcaggtc gatgctggga ggagtgctct ggcaggagcg
     8761 cgacagctcc actgaacagg catcggatat gagacctgtc acaacaaggc agccttctga
     8821 agaggagtac gctgacatgg tatttgccaa caagattgtg aagcacagcc ggtcaaatgc
     8881 aattgtgctg gcaaaaagcc ggcaactgtg tgccagcggg atcgggcaga catcaaggat
     8941 cgatgccctt cgccaggctg ttgcgaaggc aaagactttc ggcttctcac tcgaaggcgc
     9001 agtgatggct tctgacgcat tcttcccctt tgctgactcg gtcaccaccg cacatgaggc
     9061 gggtatttca gccgttgttc agccgggagg ttccgtacgt gatcaggaat caatcgatta
     9121 ttgcaataaa acaggcgttg caatggtttt tacaggtata aggcatttca aacattaaga
     9181 tagactaaca agaaacagtt tcacggaccg atgggatttt tttcttttct ttcacaggag
     9241 gttgctatag accttggcac agccaataca aatataatcg tcaatgacaa gctcgtggtt
     9301 gatgagccat ctattgtggc cattgacagg aatacaggca ggcttatagc aatcggtgac
     9361 agggccaggc agatgcatgg caggactcat gagaacatac gggtaatcag gccgctgcgt
     9421 gatggtgtca ttgctgactt caatgctgcc gagctgatga taaggggcat gatcaggatg
     9481 atcagcagcg ggcccaaatt cttttcaccg tcgctgaaga tggttgttgg tatcccatgc
     9541 ggaagcaccg aggtggggat aagggctgtc cgtgactcct cagagcacgc cggaggaagg
     9601 gatgtctacc tgatctatga gccaatggct gctgcagtag gtatcggcct tgacgtggaa
     9661 gcgcccgagg gatgcatggt ggtggatatc ggaggcggaa ccacggagat agccgtcata
     9721 gcgctgggcg gcattgtatg caacaggtcg ataagggtgg ccggtgacgg ctttacctct
     9781 gacatacagg cttacatgcg tcaccagcat aatatcaaga tcggtgacaa gactgccgaa
     9841 gatatcaaga ttgccgtggg agcagctctt cctgacattg aagacccgcc tgccgacttc
     9901 gttgtacggg gccccaacct gatgacctcg ctacccatag agatacccgt atcataccag
     9961 gagatcgcct actgtcttga caagtctctt accaaaattg aggcatcgat ccttcaggta
    10021 ctcgagcaga ctcctcccga actgtactct gacattgtca acaagggagt gttccttgca
    10081 ggcggcggcg ctctgctgag aggccttgac aagcgtctca ctgacaagat caacatcccc
    10141 ttcagggtgg cggaagaccc gcttcactct gttgtcaggg gcaccggtgt ggcgctgaag
    10201 aatgtagaga aatacccgtt cctgatgaga taaaccccgt tcatgaatga gaagccttct
    10261 gaacttcctg ctcaggttta agaccctgat ccttttcctt gtgctggaag cggtggctct
    10321 tgtcatgata tcaacatcac acaattacca tcagaccgtc ttctacggcc tgtcacgcaa
    10381 tatttccgga tttgttgcca ggagggtgga aagtgccact tactatttcc gcctgcgaag
    10441 tgtaaacgaa gagcttgtca gggaaaacac cctgctgcgg agacgccttg aggagctgac
    10501 cgccaggccg gcggaaggat ttaccggagc agatgattcc atcagcggtg tcaattacac
    10561 atacatgagt gccagggtca tcagcaattc agtcaacaaa cagaaaaact tcattaccct
    10621 tgacaggggg tcaaagcacg gagtagaagt tggcatgggg gtagcctccc ctgcaggagt
    10681 ggtaggagtg gtggtgggca cctcgccaaa ctattcggtg gtgatgtcgc tgcttaacat
    10741 tgatttccgc ctcagtacaa gtattgcccg caatgactat ttcggatcac tggcatggga
    10801 cggcataaat taccggtatg caaagctctc ggagattcct catcatgtga cggtaagtga
    10861 gggtgataca attattacaa gcggctattc agccatcttt ccacccggtt tgatggccgg
    10921 aacagttacc ggtgatcaga agcggggtgg cgacttcgtc tctatgaagg ttatgctctc
    10981 ggtagatttc aagaagctta ccgatgttta cgtaataggc aacctgacca gggaggagtg
    11041 ggaaaacctt gaggaggagg tggtaagatg aacagtttct tgaggtacac gggcgccttt
    11101 gtgctgctgg tagcgctgca gctcctcata ttcaacaata ttgagttcag cgggtatgtc
    11161 aacccttatg tgtacgtgat gtttatcctg atcctgccgg tgtcaatacc ctcatggata
    11221 cttctcctgc tgggctttct taccggcttt gtgatcgata tttttagcgg aaccatgggt
    11281 gttcatacct ttgcaaccgt acttgccggc ttcgtccgcc catgggtgtt gtcactcaat
    11341 gttaccgccg aagctgccga ggatgacatg tctccctctg ccaggcgaag cggactgcga
    11401 tggttcctgg tttataccgt tacagtggtg ctggtgcatc acctggctct cttctttgtg
    11461 gagctgttca gcttcaggta tttcttccac actctgctga gagtgttgct gagcactgcg
    11521 gttaccacat tcttcattct gcttttcgac ttcatcaggc cacacagatg aggatgcaga
    11581 tatgaaggac cggtatgcac acaggaaata cgttatcatg gcgttggtgc ttatggcggc
    11641 catgatactc attgcccggc ttttctatat ccagatagtt gacaagacat accgggtatc
    11701 tgctgccaac aacgcactgc ggcctgtaac gcaatacccg gcacgcgggc ttatctatga
    11761 ccggaacggc aaactgctgg tatacaacca ggcagcctat gacctgctgg tgatacccgt
    11821 ccagaccagc gccttcgata ccacaaggtt caacgagatc cttggtatca ccatggatga
    11881 tttcaagacc aggatgaagg ctgccatatc ctattcgcgc cgggcgcctt cagtcttcat
    11941 gaagatgata tcttcagaga catacgccat attgcaggag gagctttacc tcttccccgg
    12001 cttctacgtg cagcccagaa ccctcaggaa gtatacccgg ccggtggcag ctcacctgtt
    12061 gggctacgtg ggcgaggttg acaacaagct gatagcaaag gacaagtatt acaggtcggg
    12121 cgactatata ggcgtcagcg gtattgagaa atcatacgag gagcatctca ggggccggaa
    12181 gggggtcaac atattccttg ttg
//
LOCUS       DAOPBK010000072        12175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5174_length_12175_cov_4.524010, whole genome shotgun sequence.
ACCESSION   DAOPBK010000072 DAOPBK010000000
VERSION     DAOPBK010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5174_length_12175_cov_4.524010"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PLA17_05715"
     CDS             complement(<1..1116)
                     /locus_tag="PLA17_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPK84945.1"
                     /translation="MIWFPTSKKEMEAAGWERPDVILFTGDAYIDHPSFGAAVISRVL
                     ESAGMRVAVVPQPNWRDDLRDFTKLGEPRYFFAVTAGNMDSMVNHYTASRRLRSDDAY
                     TPGGIAGFRPDYATVVYTQILKKLYPSSSVLIGGIEASMRRLMHYDYWRDRLEPSILI
                     SSGADLLIYGMAERAVAQLAGRLLAGERMKEIHDLPQSAFTVSSDTVLPALPGVRDKA
                     LRPCHEALASRKAYAENFVLFEKTSNSIKPVRLTEQCGDVTVVVNPPLPPATEAETDA
                     VYDLLFNYAPHPRYKKKGAIPAWEMIKFSVNIHRGCFGGCSFCAIAAHQGKFISSRSE
                     ASVMREVERISRMDGFKGYLSDLGGPSANMYKMAGRER"
     gene            1411..3642
                     /locus_tag="PLA17_05720"
     CDS             1411..3642
                     /locus_tag="PLA17_05720"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPK84946.1"
                     /translation="MIFALPGNLSLLSAQKNSTRPKVGLALSGGGAKGLAHLGVIKVM
                     EEAGLKPDYVTGVSMGSIVGGMYAMGYTPDSIARMFSMFNWEYAMSDMIPENKIIFHE
                     KRYFHNSIIALPITRDAIKIPSGLISGQQIESGLNHYFWPAATITDFSKLPVPFLCLA
                     TDVVTSKKVVLQSGYLPDAIRASMAIPSVFTPVKTDTAILVDGGVVRNYAATELREMG
                     ADIVIGSYVSFKGYGEKDLESAYGILKQIGFLSSLADYEEQKQQTDILIEPELGEVGT
                     LAFSNVDTIISRGYHEALEYRDIFKKLADSLDAIGPRNPVKALPDVRYYVFDNIRVTG
                     NEKISDDQIIGILDISPGENVDRDLLEERIELLYGKNWFEKVRYMILPGDEGLTLEIN
                     CIERPRAMLYGSLYYDPVLSAGAVISISARDLVVTRSVINLDSYIGQYYRGRLSAIQY
                     IDKSQKTGLEFSLFTDNTRLPLIKLKDETIPMLSQNFNISLSLSKRLSLNHLMSLTAS
                     FENRHLVPDFLTSTMINRLTYDYLRLNYSYKVNSVDRKYFPRQGITSGISLSVLKMTR
                     GVVKTGSRRSVYLPGNEYSPFSFDRNYSARAWINSYSPVSGKVTLLVGGDLLLTTGTD
                     SITSGNDFWLLGGITPLTDRSIAAVGFHPNQITVRNAGGLRLGTDIEVTRDLHLAFDL
                     NLFALKEPDRETGFTIIGGYAIGAGYMTVVGPVRIGIMHGLYDKQILYRPVKGYVSLG
                     FNF"
     gene            3703..5943
                     /locus_tag="PLA17_05725"
     CDS             3703..5943
                     /locus_tag="PLA17_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK84947.1"
                     /translation="MNSNTVIQKHRRVCNLIVQKKVSQSFEIMSDMLDNVSVNDLREQ
                     FSELQMTYRNILRYTVEGIQDPEREKVYNRLLQSILRLNDKIKQDILARYSGWYTYSV
                     RASEQKEERLWGGNIIQSVDDLVFKSRLDDLLASAEISLPDPQSEAAMESERLSGIIF
                     NLLWLTDYYGEAEESLIDRLIGSARFEWHEIATFVSAVTLSSLRVWDPAKIRLLARLT
                     ADQTPQVAERAVTGLVFSLHYYNDRLNLYPEVIQTVRELAKLKNFRERCRIVVLQAIR
                     SRYTEQLSRRMNEEILPKVVKLKPRIEEKLDLDRILGDNPEEGLNPDWSEMFRGSEEM
                     FKTIEELTNLQMEGSDVYMSAFAGLKKFEFFRKLRNWFIPFYPDHEAVDSIFRDEILG
                     PGINELAEALYKTPFICNSDKFSLILNMKHMPSDQKSMMLKVFRMELDGLEQMKYDNE
                     LTDPAAVFRTTVTQYIQDLYRFFKLSDFRHEFDDFFTGRLDLYNSLFYRETCGSVADD
                     RSLADYFFSKEYYEDALALYLTILKSVTDDAELYEKAGYCYQKAGEYDKALEKYSMAT
                     IIEPRTWTLRKTGWCLRRLGKPAEALEAYRRALQNEPDDLNTVLMVAHCCLDTANYDE
                     ALKHYFRVEYESPGNSKVLRPIAWCYLVTGRYAEAEMYFERLAETGLTPYDKINMGHL
                     ALCQGNTRIAAGHYLSALAGGEMSAESFLSAFNDDIPFLAENGVNPDDLPILLDYVLM
                     NLKKEN"
     gene            complement(5879..7921)
                     /locus_tag="PLA17_05730"
     CDS             complement(5879..7921)
                     /locus_tag="PLA17_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPK84948.1"
                     /translation="MEEKKRFFSWWRAASLLFLAALLMPASLESQTQTPVPVPVTISG
                     EKIVTDGRVYYMHQVLKGQTLYSIARAYSVSVDLVARENGLVDNIISDGQVLRIPAAA
                     VEQGQAAMKNYQTGTQYGAAGQTRQAQQAGQAGPGQGRPAVAGTGTQMPQKNSPQDDY
                     IYHRVKRGETLGTIAGDYGITVRELKRANRGLLFPHEGDYLLIPKRKASYRSPDQRAV
                     PEQMPAGVVAEIPADTVMTDTLTVLDEPEVFTVPGEKTRIDRLHGTVRVAVLFPFFVR
                     ENNAMVYADTTKRVGTTTQVYDGSLPFLEAYEGVLIAADSLRSLGLNVEMKVFDTRAD
                     SAEIKRLIRTGELDNADLIIGPVFSYNLEQIAGWAGERDIPVVSPVPLRDSHIHENKP
                     SLYRVFPSQEVVQDLIADEVASRQGSRVIFLYSDTAMYDPATARLWQKVKTAASDTGF
                     SDGGSIVPFYYTGVTERRNVYGTVASFESQLDPERKNIIVLATTSTPVVSSAFSALHS
                     LTKRYDIMVIGYPEIRGLETVDLKYYYDLELHIPSESYVDFESPGVKSYSAIYLKKFR
                     TEPMAESFAWRGFDIAWYFIGGIAIGGKGFLKDPGTFNPELLCLEPDFRRNSRNNGYE
                     NRGMFILHYRKDMTIEILKPGHQAPEEEMNDRKERVSFPFSGSSERNRARWEGRQD"
     gene            complement(7960..8919)
                     /locus_tag="PLA17_05735"
     CDS             complement(7960..8919)
                     /locus_tag="PLA17_05735"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPK84949.1"
                     /translation="MAKAITGTDFNFPRQKGVYRGKVRDVYNIDDKYLLMVATDRISA
                     FDVVLPEGIPYKGQVLNQIATMFLEMVSDIVPVWNIASPDPNVTVGVRCQPYPVEMIV
                     RGYLTGSSWRLYRGGGREICGIPLPEGMREHQKFEKPLITPTTKAEQGMHDQDISAEA
                     IISSGLVPEEEYRKLEEYSMKLFMRGSEIAARQGLTLVDTKYEFGKRDGKIYLIDEIH
                     TPDSSRYFYSDGYEERFARGEHQRQLSKEFVREWLMANGFQGRSGEKVPQMTPEIVME
                     ISDRYIELYENITGKKFVKADMEDPLRRIERNVNSYLKQLQVR"
     gene            complement(8931..9902)
                     /locus_tag="PLA17_05740"
     CDS             complement(8931..9902)
                     /locus_tag="PLA17_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPK84950.1"
                     /translation="MTEQVILLDGIDPMVMFGPGNRLLDQIQGCFPKVRLIARGNEIK
                     CVGEADEVSVFVSRMNALIDYYRRYNRLDEDDIERLILDDEHMRSASNGDFEDALIFG
                     TSGRPIRARTVNQLQLVKEYAGNDLIIAEGPAGTGKTYTAIALAVRALKNHAVKKIVL
                     TRPAVEAGERLGFLPGDMKDKLDPYLQPLYDALHDMIPYRKLEQWIGDGTVQIAPLAF
                     MRGRTLESCFVILDEAQNATMSQLKMFLTRMGVNSKFILTGDTTQIDLPKRSESGLLR
                     AMHLLEGIEGISIIRFDERDIVRHRLVRHIVRAYDSEEKNRKEESQQ"
     gene            10038..10832
                     /locus_tag="PLA17_05745"
     CDS             10038..10832
                     /locus_tag="PLA17_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPK84951.1"
                     /translation="MPLITLTTEWRENDFFNGILRGKLASACPGTLIVNNAGGIPPLN
                     IMHGAFVVRNTFIHYPEGTIHLIFVSSEGPAEHPHLLVKAHNHWFVGANNGMFNLILN
                     SEPDLIISLRNDDREDEITLFAVTAAAIANGQPPESLGERISSISEKVPLRATIDRDV
                     IIGSVIFIDSYGNAITNITREIFARVFEGRDYRILIKSNNYFTEKISRFYREEPVGEI
                     VTRFNSLDLLELSINGANLCQLFSVDTGDAVRVEARNQGRETNGLF"
     gene            10845..11633
                     /gene="mazG"
                     /locus_tag="PLA17_05750"
     CDS             10845..11633
                     /gene="mazG"
                     /locus_tag="PLA17_05750"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPK84952.1"
                     /translation="MRKKAIREFARLLDIMDELRTKCPWDMKQTNQTLRKLTIEETYE
                     LADAILQDDNDAIRKELGDLMLHVTFYAKIGSEKELFDMGDVLEGINNKLVYRHPHVF
                     GDVNVTRGAREVEENWEKLKMTEHTEYKPVLSGVPVSLPATIKANRIQEKVRGVGFDW
                     NSREQIWDKVTEELDELKQAIVSGKKVDIESELGDLFFSLINAARLYEIDPESALEKT
                     NRRFMKRFNYLEQKTLAKNLSLHDLTLDEMNEIWEEAKQFDKPL"
     gene            complement(11630..>12175)
                     /locus_tag="PLA17_05755"
     CDS             complement(11630..>12175)
                     /locus_tag="PLA17_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HPK84953.1"
                     /translation="HTVSIGAFRAASEAFTDLAILQLDAHSDLRPFYEGSHLNHACVA
                     ARAKEYAPLVQVGIRSMSAEELPFADRRSIFFAHELYRDKTLYNKALDLLPGNVYITV
                     DLDVFDPSVMPSTGTPEPGGPDYRELMFFLRDVFSKRNVVGFDVVELCPSESNKAPDF
                     MAAKVVYQMLSYRFSAQPVSS"
BASE COUNT         3178 a         3117 c         2903 g         2977 t
ORIGIN      
        1 cctctccctc ccggccattt tgtacatgtt ggccgaaggt ccgcccaggt ctgacagata
       61 acctttaaac ccatccatcc ggcttatcct ctcaacctcg cgcattaccg aggcctctga
      121 acggctgctg atgaacttgc cctggtgagc agcgatggca caaaaactgc atcctccgaa
      181 acaaccacgg tgaatattga ctgagaactt tatcatctcc catgcaggga ttgccccttt
      241 ctttttatac ctggggtgag gagcgtaatt gaagagcagg tcatagacag catcagtttc
      301 agcctctgtg gcggggggaa gaggcggatt gaccaccaca gtaacatctc cgcactgctc
      361 ggtgagcctg acaggcttaa tgctgttgct tgtcttctcg aacaggacaa agttttcagc
      421 atacgctttt ctggaagcga gagcctcatg acacggcctc agcgccttat ccctgacccc
      481 cggaagagcc ggcaggacag tatccgatga aactgtgaag gccgactggg gcaggtcatg
      541 tatctccttc attctttcgc cggccaggag ccttccggca agctgcgcaa ctgccctttc
      601 agccatgcca taaatgagca ggtcagcacc gctggagatc agaattgaag gttcaagcct
      661 gtcacgccag tagtcataat gcatcaggcg gcgcatcgat gcttcaatgc cccctatgag
      721 caccgaagag gaaggataga gcttcttaag tatctgagta taaacaacag tcgcgtaatc
      781 aggcctgaat cctgcgattc ctccgggagt atatgcatca tctgatctca gcctgcgcga
      841 tgcagtgtaa tgattgacca tcgagtccat gttgcccgcg gtgactgcaa agaaataacg
      901 gggctctccc agcttggtga aatcacgcag gtcatccctc cagttgggct ggggcacaac
      961 ggcaaccctc atgcctgctg actcaagaac gcgggaaatt actgctgccc cgaaagaagg
     1021 gtggtcaatg taggcatcgc cggtaaacag tatgacatca ggtctctccc accctgctgc
     1081 ctccatctcc ttcttcgaag ttgggaacca gatcatcgta cttttctccc tgtttttcac
     1141 ctgcacaagt tacatataat ggataaattg ctcccaaatg taacagaaaa gtgctacctt
     1201 cacttgtcag cgtcaattat caggtcaatg tgcggacaag tcaacagatt gagagatacg
     1261 gtggcgccat tgcagccata accatgcaaa aaaatgtgtg gacaagtcaa tagattgaaa
     1321 gatgcggcgg caccgttgca gccatcatca tgcaaaaaaa tgtgcggaca aatcaataga
     1381 ttgagatgta cggcattatg tctgttgctg ttgatttttg cgttacccgg aaatctgtca
     1441 ttgctttccg cacagaaaaa cagcaccagg ccaaaggtgg gtctggccct cagcggcgga
     1501 ggcgcgaagg gtctcgctca cctgggggta ataaaggtaa tggaggaggc aggcctgaaa
     1561 cctgactatg tcaccggggt gagcatggga agcattgtag gagggatgta cgccatgggc
     1621 tacacccctg acagcatagc acgcatgttc agtatgttca actgggaata tgcaatgtca
     1681 gacatgatcc cggaaaacaa gatcatcttt catgagaaga gatattttca taacagcatt
     1741 attgccttac caatcacccg tgacgcaata aagatcccat cgggactgat cagcggccag
     1801 cagatagaga gtggtctgaa ccattatttc tggcctgcag ccacaattac cgatttttcc
     1861 aaactccccg tccccttcct ctgtctggca actgatgtgg taaccagtaa aaaggtggtt
     1921 ttacaaagtg gttacctccc ggatgccata cgggcgagca tggccatccc ctcagtattc
     1981 acccccgtca agacagatac ggcaatcctg gttgacggcg gtgtggtacg caattatgct
     2041 gcaaccgaac tgagggagat gggagcagat attgtgatag gttcatatgt gagcttcaaa
     2101 ggatacgggg agaaagacct ggaatccgcc tacggaatac tgaagcagat cggattcctt
     2161 agcagtctgg ctgactatga ggagcagaag cagcagactg acatcctgat tgagccggaa
     2221 ctgggagaag tgggtactct tgcattcagc aatgtagata ccattatttc cagaggctat
     2281 catgaagccc ttgaatacag agatatcttt aaaaagcttg ccgactccct tgatgccatc
     2341 ggtccgcgta acccggtaaa ggcactgccc gacgtccgtt attatgtttt tgataatatc
     2401 agggtcacag gaaacgagaa aatcagtgat gaccagatta ttggcatcct tgatataagc
     2461 cccggagaaa atgttgacag ggatctcctg gaagagagaa ttgaactgct gtacgggaaa
     2521 aactggtttg aaaaggtcag gtacatgatc ctacccggag atgaagggct gacactggaa
     2581 atcaattgca ttgaacggcc ccgggcaatg ctgtacgggt cactctatta tgacccggtt
     2641 ctcagtgcag gtgcagtgat aagcatctca gccagagacc ttgtcgtcac acggtcagtc
     2701 attaaccttg attcatatat tggtcagtac tatcgcggca ggctgagtgc gattcagtac
     2761 atagacaaaa gccaaaaaac agggcttgaa ttctcactat tcactgacaa tacccggctg
     2821 ccactcatta aactgaaaga tgagacaata ccgatgctca gccagaattt caatatttca
     2881 ctgagcctga gcaaacgact gagcctgaac cacctgatga gcctgacagc ttcgtttgaa
     2941 aaccggcacc tcgtccctga ctttcttaca tcaaccatga taaacaggtt gacatatgat
     3001 tacctcagac tgaactacag ctacaaggtc aactccgttg acaggaaata ctttcccagg
     3061 cagggtataa cttccggaat ctccctgtca gtactcaaaa tgaccagagg tgtggtcaaa
     3121 accgggtcaa gaaggtcagt ctatctcccg ggtaatgaat attcaccatt cagctttgac
     3181 cgcaactatt cggcaagggc atggattaat tcctattcac cggtttcagg gaaagtgaca
     3241 ttactggtcg gtggtgacct gttgttaact acaggcacag attccataac ctcgggaaac
     3301 gacttctggc ttcttggcgg aatcaccccc ctgactgacc gctccatagc tgctgtcgga
     3361 ttccacccaa accagattac tgtcaggaat gccggaggat tgcgtctcgg aaccgacatt
     3421 gaagtaacca gggaccttca cctcgcgttt gatctgaacc tgttcgccct gaaggagcca
     3481 gaccgcgaga ccggtttcac cattatagga ggttacgcaa taggtgcagg ttatatgaca
     3541 gttgtcggcc ctgtccggat tggaatcatg cacggcctgt acgacaagca gatactctac
     3601 aggcctgtaa agggatacgt aagccttgga ttcaacttct gaaaccgggg aaatggcctg
     3661 attgttgtct tattactgat ggcggtcatc acgacacgta ttatgaatag caatacagtc
     3721 atacagaaac acaggagagt ctgcaacctc atcgttcaga aaaaggtgag ccagagcttt
     3781 gaaatcatgt cagatatgct tgataatgtg tctgttaatg atctcaggga gcagttctcc
     3841 gagctgcaga tgacttacag gaatattctc cggtacactg ttgagggcat acaagacccc
     3901 gaaagggaga aggtttacaa ccggctcctg cagagtatac tcagactcaa tgacaaaatc
     3961 aaacaggaca ttttggcacg ttattcagga tggtacactt attctgtcag agccagtgag
     4021 caaaaggaag agaggctctg gggagggaat atcatccaga gtgtggatga ccttgtattc
     4081 aaatcacgcc ttgatgatct ccttgcatct gcagagataa gtctgcctga tccgcagagt
     4141 gaggcagcaa tggagagtga acgcctgtca gggattatat ttaaccttct gtggctgacc
     4201 gattattacg gtgaagccga agagagtctc atcgatcgcc ttataggatc agcccgcttt
     4261 gagtggcacg agatagcaac attcgtcagt gctgttacgc tgagcagtct gcgggtctgg
     4321 gacccggcca agatcaggct gctggccagg ctgacagctg accagactcc acaggtggct
     4381 gaaagagcgg ttacggggct ggtcttctca cttcattatt ataatgaccg cctgaacctc
     4441 tatcctgagg ttattcagac agtcagagaa ctggcaaaat taaagaactt cagggagaga
     4501 tgccgcatag tggtgcttca ggctatcaga tcacgctata cagagcagct cagccgcagg
     4561 atgaatgagg aaatacttcc gaaagttgtc aaacttaaac caaggatcga agaaaaactg
     4621 gaccttgaca gaattctggg cgataacccc gaagagggac tgaaccctga ctggtcagaa
     4681 atgttccgcg gatcagagga aatgtttaaa acgattgagg aactgaccaa tctccagatg
     4741 gaaggatcag atgtttatat gtcagcattt gcaggattga aaaagttcga attcttcagg
     4801 aagctgcgca actggtttat ccccttctac cctgatcatg aggcagtaga ctctatcttc
     4861 agggatgaaa tactcggccc gggcataaac gaactggctg aagcgctgta caaaaccccg
     4921 ttcatatgca actctgacaa attctctctc atccttaata tgaaacatat gccatctgac
     4981 cagaagagta tgatgctcaa ggtattcagg atggaacttg acggactgga acagatgaaa
     5041 tatgacaatg agctcaccga tcctgctgca gttttcagaa ctacggtgac acagtatatt
     5101 caggatctct accggttctt caagctctct gatttcaggc atgagtttga tgatttcttc
     5161 accggaaggc ttgatctgta caactccctt ttttacaggg aaacatgcgg ctcagttgct
     5221 gatgacaggt cactggccga ctatttcttc agcaaggagt attatgagga tgcactggct
     5281 ctttatctga ccatcctgaa gagcgtcacc gatgatgcgg aactgtacga aaaggcaggt
     5341 tactgctatc agaaggcagg agaatatgac aaagccctgg agaagtactc catggcaact
     5401 attattgagc cccgtacatg gacgcttcgc aagacaggat ggtgccttcg caggctaggc
     5461 aaaccggccg aagccctgga ggcataccgc agggcactgc agaatgagcc tgacgacctg
     5521 aatactgtcc tcatggttgc ccactgctgc cttgatactg ccaattacga tgaagcgctg
     5581 aaacactact tcagggtgga gtatgaaagc cccggcaatt caaaggtact gaggccaata
     5641 gcctggtgtt acctggtcac cggaaggtat gccgaagctg agatgtactt tgagaggctt
     5701 gcagagaccg gcctgacacc ctatgataaa ataaatatgg ggcaccttgc actttgccag
     5761 ggcaacacaa ggattgctgc cggacattat ttaagtgccc tggccggagg agaaatgtct
     5821 gcagagagct tcctgtcagc gtttaatgat gatattccct tcctggcaga aaatggcgtc
     5881 aatcctgacg accttcccat cttgctcgat tacgttctga tgaacctgaa aaaggaaaac
     5941 tgaccctctc tttccggtca ttcatctctt cttcaggagc ctgatgaccg ggctttaata
     6001 tttctatggt catgtctttg cggtaatgaa ggatgaacat ccccctgttc tcatagccgt
     6061 tattacgact gttgcgccgg aagtcgggtt ccagacacag aagctcagga ttaaaggtgc
     6121 cggggtcctt cagaaaaccc ttcccgccga tggctatacc accgataaag taccatgcga
     6181 tgtcaaaacc gcgccatgca aagctttcag ccatgggctc ggtcctgaat ttcttcagat
     6241 agatggctga gtatgatttt accccgggtg actcaaagtc gacatagctt tctgacggta
     6301 tatgcagctc aagatcataa taatacttca ggtcaactgt ttcaagaccg cgtatctcag
     6361 gatacccgat caccattata tcatatcgct ttgtcagact gtgaagtgcg gagaatgccg
     6421 aactgactac aggtgtactg gttgtggcca gcactatgat gtttttccgt tcaggatcaa
     6481 gctggctttc aaatgaagca accgtgccgt aaacatttcg tctttccgtc accccggtat
     6541 agtaaaacgg cacaatactc cctccgtctg agaagccagt atcactggca gccgttttca
     6601 ctttctgcca cagtctggca gttgccggat catacatggc cgtatcggaa tagaggaaga
     6661 tgaccctgct tccctggcgt gacgctactt catcggcgat taaatcctgc accacttcct
     6721 gacttgggaa aaccctgtac agcgaaggct tgttctcatg aatatgactg tcgcgaagcg
     6781 gcaccggtga tacaacaggt atatcccgtt ctccggccca gccggcgatc tgctccaggt
     6841 tataggaaaa cacggggccg attatcaggt ctgcgttatc cagttctcct gtccggatca
     6901 gtcttttgat ttctgcagag tcagccctgg tatcaaaaac cttcatctcg acgttcaggc
     6961 ccagtgaccg caggctgtca gcagctatca gcacgccttc ataggcttca aggaatggga
     7021 ggcttccgtc ataaacctga gtggttgtcc caaccctctt agtggtatct gcatatacca
     7081 tggcattgtt ttcccttaca aagaatggaa acagcactgc caccctgaca gttccgtgca
     7141 atctgtcaat cctggttttt tctccaggca cggtaaagac ctccggttca tccaggacgg
     7201 tcagggtatc tgtcatgacc gtatcagcag gtatttctgc aaccactccc gcaggcatct
     7261 gctccggaac cgctctttga tccgggctcc tgtaggaagc cttgcgcttg ggtataagga
     7321 ggtaatcacc ctcgtgaggg aagagcagcc ccctgttggc ccttttcagc tctctaaccg
     7381 taataccgta atctccggcg attgttccca gtgtctctcc tctttttacc cggtgatata
     7441 tataatcatc ctggggagaa ttcttctgtg gcatctgtgt acctgtaccg gcgacagccg
     7501 gcctgccctg accgggtccg gcctggcctg cctgttgtgc ctgcctggtt tgtcctgccg
     7561 caccgtactg tgtgccggtc tggtagttct tcattgcagc ctgcccctgt tccactgcag
     7621 cagcaggtat cctcagcacc tggccatcac ttatgatatt gtcaaccaat ccgttttcgc
     7681 gggccaccag gtcaacactc acactgtaag ccctggctat tgaatagagc gtctgaccct
     7741 tcaacacctg gtgcatgtaa taaaccctgc catccgttac aatcttctct ccggagatgg
     7801 taacaggtac cgggaccggg gtctgtgtct gggattcaag agacgcaggc atcagcagtg
     7861 ctgcaagaaa cagcagggaa gctgcccgcc accaggagaa aaacctcttt ttttcttcca
     7921 tcaattgatt cattcgtatt actgtcaccc tcccctgtat tatctcacct gcagctgttt
     7981 aagatagctg ttgacattcc tctctatcct tctgagggga tcttccatat cagcctttac
     8041 aaatttcttg ccggtaatgt tttcatagag ctcaatgtaa cggtctgata tctccatgac
     8101 tatttcaggt gtcatctgcg gcaccttttc accgcttctt ccctggaaac cgtttgccat
     8161 aagccactca cgcacaaact cttttgagag ctgcctctgg tgttcgccac gggcaaatct
     8221 ctcttcataa ccatctgaat aaaagtatct tgaactgtcg ggagtatgga tctcatcaat
     8281 aagatatatt ttcccgtccc gtttcccgaa ctcatatttg gtatcaacaa gcgtcagccc
     8341 ctgccttgcg gctatttccg agcctctcat gaacagcttc atcgaatact cctcaagctt
     8401 cctgtattcc tcctccggaa ccaggccgct gctgatgatt gcctctgcgg agatatcctg
     8461 atcatgcatc ccctgttcag ccttggtggt aggcgtaatg agaggttttt cgaacttctg
     8521 gtgttccctc atcccttcag gaagaggaat tccgcatatc tcccgtccgc ctcccctgta
     8581 aagcctccat gaacttcccg tcaggtaacc ccttactatc atctcaacgg gatatggctg
     8641 gcatctgacc ccgacagtta cattggggtc cggagatgca atattccata ccggcacgat
     8701 atcactcacc atctcaagga acatagttgc tatctgattg agaacctgtc ccttgtatgg
     8761 aatgccctca ggcagcacaa cgtcaaatgc cgagatacgg tcagtggcca ccatcaggag
     8821 gtatttgtca tcaatattat agacgtcacg aactttcccc cggtatactc ccttctgccg
     8881 cgggaaattg aaatctgttc ctgtaatagc ctttgccatg gatcggttaa ttattgctgc
     8941 gattcttcct ttctgttttt ctcctcgctg tcataagccc tcactatatg cctgacaagc
     9001 ctgtgtctta ctatatccct ctcatcaaaa cggataattg aaatcccttc aataccttcc
     9061 aggaggtgca ttgcccggag caatcccgac tcgctccgtt tcgggaggtc aatctgggtg
     9121 gtatcgcctg taaggatgaa cttggaattc acgcccattc ttgtgaggaa catcttgagc
     9181 tggctcatgg ttgcattctg ggcctcgtca agtatcacaa aacagctctc gagtgttctt
     9241 cccctcatga acgccagagg tgcaatctgc accgttccat cccctatcca ctgctcgagc
     9301 ttgcggtacg gtatcatgtc atgcagggca tcatagagag gctggagata aggatcaagc
     9361 ttgtctttca tatcaccagg caggaagccc agccgttctc ctgcctcaac ggcaggacgg
     9421 gtgagcacaa tcttcttaac agcatggttc ttcagagccc tgacagccaa agcaatggca
     9481 gtgtatgtct tcccggtgcc ggccggaccc tcggcaatga tcaggtcatt gccggcatac
     9541 tccttcacca gctgaagctg gttgacagtg cgtgccctga tagggcgccc actggtgcca
     9601 aatataagag catcctcgaa atcaccgttg gaggctgacc gcatgtgttc gtcatcaagg
     9661 atcagacgct caatatcatc ctcatcaagc ctgttgtaac gcctgtaata atcaattagt
     9721 gcattcattc gcgagacgaa aacggaaacc tcgtcagcct ctcccacaca tttgatctca
     9781 tttcccctgg ctatcagcct gactttcgga aaacaaccct gaatctgatc aagaagcctg
     9841 ttaccaggcc caaacatgac catcggatca ataccgtcaa gtaaaattac ctgttccgtc
     9901 atctgattct cttctccaaa tctttctaaa ggactctcct taagcttctt atatctgtga
     9961 tcttttgtgt gcatccattc ttccgcaaag taagtttatt ttttgcagaa gactatcttt
    10021 gctgtaacaa ttttaggatg ccacttatca cgttaaccac cgagtggagg gagaatgatt
    10081 tcttcaacgg gatactcagg gggaaacttg cctccgcctg ccccggcact ctcattgtga
    10141 acaatgccgg aggaatccct ccactgaaca taatgcacgg cgctttcgtc gtaagaaaca
    10201 ccttcatcca ttatcccgaa ggcaccatcc acctgatatt cgtcagcagc gagggacctg
    10261 cagagcaccc acaccttctg gtcaaagccc acaaccattg gtttgtgggt gcaaacaacg
    10321 gaatgttcaa cctgattctc aattccgaac ctgacctgat catcagcctc aggaatgatg
    10381 acagggaaga cgagataact ctttttgccg tcaccgccgc ggccatagcc aacgggcaac
    10441 cacctgagag cctcggggaa agaatctcct ccatatccga aaaagtaccg ctgagagcta
    10501 caattgaccg cgatgtgata ataggcagcg ttatcttcat tgactcatac ggcaacgcta
    10561 tcaccaacat tacccgggag atcttcgcca gagtcttcga gggaagggat tacaggattc
    10621 tgatcaagag caacaactat tttaccgaaa aaataagcag gttctaccgt gaagaacctg
    10681 tcggggagat cgtgacccgc ttcaactcac tcgaccttct cgaactttca atcaacgggg
    10741 caaacctgtg ccagcttttt tccgtcgata ccggtgacgc cgtcagggtt gaagcaagga
    10801 accagggtcg tgaaacaaac ggcctctttt aagaacttta acaaatgaga aaaaaagcta
    10861 tcagggaatt tgccaggctg ctcgatatca tggatgagct ccggacaaaa tgcccgtggg
    10921 acatgaaaca gaccaatcag acactcagaa aactgacgat agaggaaaca tatgagctgg
    10981 cagatgccat actgcaggat gacaatgatg ctatcaggaa agagcttggc gacctgatgc
    11041 tccacgtgac attctatgcg aagataggat cggagaaaga actctttgac atgggcgatg
    11101 tacttgaagg cataaacaac aaactggtat accgccatcc gcatgtattc ggagatgtga
    11161 atgtaacacg gggagcacgc gaagttgagg agaactggga aaaactgaag atgacggagc
    11221 acactgaata caaacccgta ctttcaggtg tgcctgtatc cctgccggca accataaaag
    11281 ccaaccgtat ccaggaaaag gtaagaggtg tgggatttga ctggaactcg cgtgagcaga
    11341 tatgggacaa ggtgacggag gagcttgatg aactcaaaca ggcaatagtc tcagggaaaa
    11401 aagtggatat tgagtctgag cttggcgacc tgttcttctc acttataaat gctgcaaggc
    11461 tatacgagat agaccctgaa tcagctctcg agaaaaccaa caggaggttt atgaagagat
    11521 tcaactatct ggaacagaaa acactggcaa aaaacctctc acttcacgac cttacccttg
    11581 atgagatgaa tgaaatctgg gaggaggcca aacagtttga taaacctctt tagctgctga
    11641 caggctgagc actgaacctg tagctcagca tctggtagac caccttcgca gccatgaaat
    11701 caggggcttt atttgattcc gaaggacaga gttccacaac atcgaaccca accacattgc
    11761 gtttgctgaa gacatcacgc aggaagaaca tcagctcccg gtagtcaggg ccgcccggct
    11821 caggagtccc tgtcgacggc atcaccgaag gatcgaacac atcgaggtca acagttatgt
    11881 agacattccc cggcagcaga tccagcgcct tgttgtaaag agtcttatcc ctgtaaagct
    11941 catgggcaaa gaagatgctc ctcctgtctg caaaagggag ttcctctgct gacatgcttc
    12001 tgataccaac ctgaaccagt ggcgcatact ccttcgccct tgcggcgacg caggcatgat
    12061 taaggtgtga cccctcataa aatggtctga ggtcagagtg agcatcaagc tgcaggattg
    12121 ccaggtctgt gaaggcttca gaggcggccc ggaaagctcc tatgctgacg gtatg
//
LOCUS       DAOPBK010000073        12051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5240_length_12051_cov_6.029677, whole genome shotgun sequence.
ACCESSION   DAOPBK010000073 DAOPBK010000000
VERSION     DAOPBK010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12051
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5240_length_12051_cov_6.029677"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLA17_05760"
     CDS             <1..260
                     /locus_tag="PLA17_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HPK84954.1"
                     /translation="LAPGKIISVTTLRISPDLSIAKAWVSIFPSEGSAAVLEVLRNRT
                     PKLRYELGQKVRNQLRIVPEIIFFIDDSNDYIDNISKLLKE"
     gene            266..1081
                     /locus_tag="PLA17_05765"
     CDS             266..1081
                     /locus_tag="PLA17_05765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK84955.1"
                     /translation="MHYDPIKELLGKVFNRHPLLRKTFYRLLNILLLRSWHVRRALKN
                     LGPSLPAVANILDAGMGFGQYSWWMASRFPSWQITAADIKNEQVADCNEFFRRTKLAT
                     RAHAIEADLVTWNSGERYDLILCVDVMEHIAEDRTVFSNFLNMTAGGGYLVISTPSDQ
                     GGSDVHSDEDTSFIGEHVRDGYAGEEITEKLRTAGYTEVSVSYTYGTPGNIAWRLSMK
                     YPVIMLSASKLFFILLPFYYLAVMPFVLALNCADLNGNHRKGTGLLVIAQRSK"
     gene            1078..2313
                     /locus_tag="PLA17_05770"
     CDS             1078..2313
                     /locus_tag="PLA17_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK84956.1"
                     /translation="MKLSLYIAKRYLFAKKSRNAINVISAISVAGVTVGTMALITVLS
                     VFNGLEDMVRSIFSTSDPEIKITPVAGKVFTPDNLMLEKLASAEGVDVYALTLEENAL
                     LRYDEQQYIATVKGVSLNYADVTDLDTAMWDGSFILQGENGNPYAVAGLGVANYLGMR
                     LNFVSPLAIYIPDRKAKIRGTPDNEFTRKYIFLSGIFAVEQEFDSKYIFLPLDFAREL
                     LAYTDEVSSIEVRMKPGADEKKTQNAIREIMGDGFRVQNRYEQQEIFYKVMKAERLAI
                     FVILSFILIIASFNIIGSLTMLIIEKERDIGILKSLGADNRLIKRIFIYEGWMISFIG
                     TIIGLLLGFILCAAQQYFGIVKLAGESLLIDAYPVRMRLADFFLVAATVLAIGYAAAW
                     YPVYYLSRRQLKEKEKAIK"
     gene            2310..3092
                     /locus_tag="PLA17_05775"
     CDS             2310..3092
                     /locus_tag="PLA17_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HPK84957.1"
                     /translation="MKTVYKIFFVTLMLMIVSCGNTENKKPSRKMRIPDDRLAAILTD
                     TYLTAGIMDLFAMRDTWAQRDSIQNYIDVIESHGYTYEQFETTMRYYFMSKPRKLSRI
                     YDRVTGNLLELETELMTQQSAVKPSEKNLWPGKPDYRLPEEFTRDPVWFDIPVDKPGE
                     YILKADIRVFEDDKSLNPRVTVYFSYRDSAGEEKRDYWQEIFLVKDGQIHNIQIKHTL
                     DTIPDARLRGWLLNHDNQSGKWEKHARISNIRIVHADDFAAQ"
     gene            3095..4198
                     /locus_tag="PLA17_05780"
     CDS             3095..4198
                     /locus_tag="PLA17_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK84958.1"
                     /translation="MRKISAQYLFTSAGKPLRRGIVTVAGDGTVTGVEDTGGSLRESA
                     STEFYNGVVIPGFVNCHSHLELSHMRGLIPAGGGLSDFIVSVRNRREASDDEIMAAAG
                     KADIEMHSGGVVACGDISNSPVTAGMSSAQSKLLAGVAEAFGLDPAVAEARMQRALEV
                     CREIRRHGLQAHVTPHSLYSLSLPLSQLIKEHIPADSIISLHFLESDDERRMAKGHAD
                     AALDLSGLASRLLLVHNTVITRDEAVKTASAGNTWFCLCPSSNIHISGKMPPAAMLRE
                     VTRRIAVGTDSLASTDRLSMIRELLLLQEAVPGITAEELIRCGTINGAMALKCDDTLG
                     SIEPGKRPGLLLVEPFDLQNMRLLPESRVRRLV"
     assembly_gap    4244..4253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4404..5186
                     /locus_tag="PLA17_05785"
     CDS             4404..5186
                     /locus_tag="PLA17_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK84959.1"
                     /translation="MPTFLFDSIVFGPVHSRRLGVSLGINLLPVTRKVCSYNCIYCEC
                     GWTPDKPDSKEKLPSRRQVRDALRKKLSEMKEQGKAPDVITYAGNGEPTLHPDFAGII
                     DDSLALRDEFFPAAKVSVLSNGSMLHRESVREALKRVDQNMLKLDSVFPDTVRQINQP
                     HVKTDLARYEEYIRELEGRFIIQTLFVRGEYEGRTVDNTTEEEITAWLEVISRLRPQL
                     VMIYTIHRDTPLGSNLRKVPESELNAIAQRVNRLGIATSVSG"
     gene            5192..6484
                     /locus_tag="PLA17_05790"
     CDS             5192..6484
                     /locus_tag="PLA17_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK84960.1"
                     /translation="MNGMRVLISPLSWGFGHAGRMIPLALALKRRGCEVIFAADALLT
                     KMIAPELPDIKTVVIPGLTIRYSRFMPQYLTIFLQLPVIVASAVREHATLRRLAKELN
                     PMVIISDNRFGFYHKKIFSVYITHQVRIAFPAFLAFLEPLAAWMHRMIISRYDLCLVP
                     DYRGSENLSGRLSHGGRLPANVAYIGPLSRFGVAGSEPSSPARAKSVPPGPGRAKGET
                     VAPGQMKRMPFAANRTKSEPVAPGQMKGEPLQTGQTENNPLQPGGGVPPAPPAQPYVC
                     LILSGPEPQRSLLLRKVSESLREFPLTVLSATPPQAPTPPEIPTPQQAPTLPEALTPP
                     KTPPPPGQAIHLITGADTTTMRQVITGASMVIARAGYTSVMELVSLGKGAVLIPTPGQ
                     TEQQYLGRHLDGRYGFITMNQNRLERLASVILPEHKKE"
     gene            complement(6461..7153)
                     /locus_tag="PLA17_05795"
     CDS             complement(6461..7153)
                     /locus_tag="PLA17_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPK84961.1"
                     /translation="MKPGENEMPDNGMAEEESRMNPGSERRLKRAAEIISVVFHPIFM
                     PLYGLLIIYSAHTLHSFLPSQIKRMILILVMANNLLLPMAFATFLYARGSIRTFNASD
                     RNERVLLLSFVLMMYSLTAFILLRMQVPTLFRAFFISIAIVTFTILLITIFYRISLHA
                     SGIGGLVVLVICMTTVYNIGMLWQSVAVALIAGAVMSSRIYLGDHTPTEVWTGVAAGA
                     VMMGLSLFFMLR"
     gene            complement(7150..8613)
                     /gene="rpoN"
                     /locus_tag="PLA17_05800"
     CDS             complement(7150..8613)
                     /gene="rpoN"
                     /locus_tag="PLA17_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPK84962.1"
                     /translation="MLDQKLQQKLLQKLSPQQIQMIKLLEVPAMQMEERIKKELEENP
                     ALEEGHEEDEVHGDEDEEEVDHEQEEREQDEFSLDDYLNDEDIPDYRLQVNNTSKDDE
                     RREDIPYAAGSSFRENLIQQFNLRTETEREKLLGEYVIGNIDNDGYLRREVENMVDDL
                     AFLQNITTDVSELDAILKIIQGLEPAGVGARNLRECLLLQIERKSQSVPSVALAKTVL
                     TDYFEEFSRRHYEKIMNRMGIEESDMKGIIDEVLKLNPKPGGTMSDPYARLTHHIIPD
                     FILEQSDSGFELSLNNRNIPELRVNRDYSEMLESYTRDKKAGKQNRDAIMFVKQKLDA
                     ARWFIDAMRQRQNTLLLTMNAILAYQEDYFAEGDDTKLKPMILKDVANMTGLDISTVS
                     RVANSKYIQTHFGIFPLKFFFSEGMQTDTGDEVSTREIKRILQECINNEDKSKPLTDE
                     KLTKILQEKGYQIARRTVAKYREQLNIPVARMRKEIT"
     gene            complement(8634..10019)
                     /gene="asnS"
                     /locus_tag="PLA17_05805"
     CDS             complement(8634..10019)
                     /gene="asnS"
                     /locus_tag="PLA17_05805"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPK84963.1"
                     /translation="MRKKVKELLAGHPTGEKVLVKGWVRTKREIKNVAFVALNDGSTI
                     NNIQIVIEINSFPAELLKEITTGASMAVTGTLVPSQGKGQNVEINASDITIYGKCDPE
                     TYPLQKKGHSMEFLREIAHLRFRTNTFGAVMRIRHAMAYAINKYFNDRGFYYLHTPII
                     TGSDAEGAGEMFHVTTLDLENLPRKKDGTVDYEQDFFGKPVNLTVSGQLEGELGALAL
                     GEIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFWDINDNMDLAEDFTKYLIRYALDNC
                     RDDLEFLNKMIDKGLIERLEFVLGNSYMRLTYTEAVRILEDAGRKWEYPVSWGSDLQA
                     EHERYLVETHFRRPVILTDYPKSIKAFYMKQNDDGKTVRAMDVLFPGIGEIIGGSQRE
                     EELDKLTRRIEELNLPLKDLWWYLDTRRFGTVPHSGFGLGFERLILFMTGMSNIRDVI
                     PFPRTPRNAEF"
     gene            10219..11061
                     /locus_tag="PLA17_05810"
     CDS             10219..11061
                     /locus_tag="PLA17_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPK84964.1"
                     /translation="MEVDSKIIVVPWDFTPVTEYALQHAIKISLMTRNPICLLHIVEK
                     ITTEGGMKAKQAAMNNRGSDVERMFGVKVKTHVTRGTIFKQIPEFVNDRNASLVVMGT
                     HGMSGMQKVTGSWALKVIAKSKVPYIVVQAPPADNEKYHDIVFPVDFRAEEKEKLSMA
                     IFMGKYFDSKIHILKAIRKDDSLAKRTNLNMNYTVRMLIQNNIEYEIREEPADKLGER
                     ALEFAQEINADLILIMTTKDITFADYIWGAKEQYIIANSSKIPVCCVNPTSAFANLGQ
                     FMYG"
     gene            11085..11795
                     /locus_tag="PLA17_05815"
     CDS             11085..11795
                     /locus_tag="PLA17_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK84965.1"
                     /translation="MSIVVSNVTKYYGAQKALDDVSFTIPAGEIVGFIGPNGAGKSTM
                     MKIITGFFPADAGEVTVCGLQAGPSAREMRRQIGYLPENNPLYLDMYVKEYLGYVAEI
                     YSPGRKSREAVSSVIERTGLTPEQGKKIGALSKGYRQRVGLAQALIHDPKVLILDEAT
                     TGLDPNQIVEIRNLISEAGKEKTVMLSTHIMQEVEAICSRVIIIDHGRIVADGPIDEI
                     SSLAGARLEETFRQLTGR"
BASE COUNT         2972 a         3150 c         3025 g         2894 t
ORIGIN      
        1 aacttgcgcc aggaaagata atctcggtga ccacgctgcg catctctcct gacctgtcaa
       61 ttgcaaaagc atgggtcagc atctttccct ccgaaggatc tgccgctgtc cttgaagttt
      121 tgcgcaaccg cacaccgaag ctaaggtatg agctgggaca aaaggtgcgc aaccagttgc
      181 gaatcgttcc cgaaatcatc ttcttcattg acgacagcaa cgactatatc gacaatattt
      241 ccaaactgct caaggaatag acccgatgca ctatgacccg atcaaggagc ttctgggaaa
      301 ggttttcaac cgccatccgc ttctgcggaa gaccttctac cggctgctga acatcctgct
      361 ccttcgctca tggcacgtca ggagggccct gaaaaacctg gggccgtcgc ttccggccgt
      421 tgcaaatatc cttgatgccg ggatgggctt cggccagtac tcgtggtgga tggcctcccg
      481 tttcccgtca tggcagatca ccgcagctga tatcaagaac gaacaggtgg ccgactgcaa
      541 cgaattcttc cgccggacaa aactcgcaac aagagcgcat gctattgagg ctgaccttgt
      601 tacctggaac agcggggaga ggtatgacct gatcctgtgt gttgacgtaa tggaacatat
      661 tgccgaagac cgcaccgttt tttccaattt cctgaatatg actgccgggg gcggttacct
      721 ggtcatctca acaccttcag accagggagg ctcggatgtc cattccgatg aagacacgtc
      781 gttcattggt gaacatgtgc gggacggata cgccggggag gagataacgg aaaagctccg
      841 gactgcagga tacacggagg tcagcgtgtc atacacctac ggcaccccgg gcaacatagc
      901 ctggaggctt tcgatgaaat atccggtcat catgctctca gcatcaaaac tcttctttat
      961 tctgctgcct ttctactatc ttgccgttat gccgttcgtg ctggcactca attgcgctga
     1021 cctgaacggt aaccaccgta agggaacagg attactggta attgcacaaa gaagcaaatg
     1081 aagctttcac tctacatagc aaaaagatac ctcttcgcga aaaaatcgcg gaacgccata
     1141 aacgtgatct cagccatctc ggtggcaggg gtgaccgtcg gaacaatggc actgataacc
     1201 gtactctcgg tctttaacgg cctggaggat atggtcagaa gcatcttcag cacctctgac
     1261 ccggagataa aaataacccc cgttgccgga aaggtcttta cccccgacaa cctcatgctg
     1321 gaaaagctgg cttccgccga aggcgttgac gtctatgcgc tgacactgga ggaaaatgca
     1381 ctcctccgct atgacgagca gcaatatatt gctacagtga agggagtgtc actcaactat
     1441 gccgatgtta ctgacctcga taccgcgatg tgggacggca gtttcatcct gcagggcgaa
     1501 aacgggaatc cttatgcagt agccggactg ggtgtggcaa actacctcgg gatgcggctt
     1561 aactttgtct ccccgctggc aatctatatt cctgaccgga aggcaaaaat caggggaaca
     1621 cctgacaatg agttcacaag gaagtatatt ttcctctcgg gaatctttgc cgttgaacag
     1681 gaattcgatt caaaatatat cttcctcccc cttgactttg caagggagct gctggcttac
     1741 accgacgagg taagctccat tgaggtgaga atgaaacccg gggcagatga gaaaaagacg
     1801 cagaatgcga tcagggagat aatgggtgac ggcttccgcg tgcaaaaccg ctatgaacag
     1861 caggagatct tttacaaggt gatgaaggct gagaggctgg caatcttcgt tatcctctca
     1921 ttcatactga tcattgcctc tttcaatatc atcgggtcac tgacgatgct gattattgaa
     1981 aaagagaggg atatcggcat actgaagagt ctgggggctg ataaccgcct gatcaaacga
     2041 atattcatct atgagggttg gatgatatcc tttatcggaa ccatcattgg cctcctgctg
     2101 ggattcatcc tctgtgcagc acagcaatac tttggcatcg taaagctggc aggtgagtca
     2161 ttgctcattg atgcctatcc cgtcaggatg cgcctggctg atttcttcct ggtggctgca
     2221 acggtgcttg caatcggcta cgcggcagca tggtacccgg tttactatct ctcgcgcagg
     2281 caacttaaag agaaggaaaa agcgatcaaa tgaaaacggt atataaaata ttctttgtga
     2341 ctctcatgct gatgattgtc tcatgcggga acacggagaa taaaaaacca tcgcggaaga
     2401 tgcgtatccc ggatgacagg cttgccgcga tactcactga cacctatctg acagcaggga
     2461 taatggatct ctttgccatg agggatacct gggctcagcg tgactcaatc cagaactata
     2521 tagatgtcat tgagagccac ggctatacct atgaacagtt tgaaaccacg atgaggtact
     2581 acttcatgag caagcccagg aagctgtcaa ggatatatga cagggtgacc gggaacctgc
     2641 tcgaactgga aacggagttg atgacacaac agagtgcggt aaagccttct gaaaaaaacc
     2701 tctggcctgg aaaacccgac taccggctgc cggaagagtt cacccgtgac cctgtctggt
     2761 tcgatatccc ggttgacaag cccggcgaat atatccttaa ggctgacatc cgcgtctttg
     2821 aagacgataa gagtcttaat ccgagggtca ccgtttactt cagctaccgt gacagcgccg
     2881 gagaggaaaa gcgtgactac tggcaggaga tcttccttgt aaaggacggg cagatccata
     2941 atattcagat aaaacatacc cttgacacta ttcccgatgc acgcctgcgc ggctggctac
     3001 ttaaccatga caaccaatcg ggtaaatggg aaaagcatgc ccgcatatcg aatatcagaa
     3061 tagtgcacgc tgacgacttc gcagcacagt gacagtgaga aaaatatctg cccaatacct
     3121 gtttacctcg gctggcaagc ccctcaggag aggcatcgtc accgttgccg gcgacggcac
     3181 cgtgaccggc gttgaagata ccggcggctc cctcagggag agtgcctcaa cggagtttta
     3241 caacggcgtc gtcataccgg gatttgtcaa ctgccattct catcttgagc tttcccatat
     3301 gcgaggcctt attcccgccg gaggcggact gagtgatttc atcgtctccg ttcgcaacag
     3361 gcgtgaggca tcagatgatg agatcatggc tgctgccggt aaggctgaca tcgagatgca
     3421 ttcaggcgga gtggtggctt gcggcgatat ctcaaacagc ccggttactg caggcatgag
     3481 ctcggcccag tccaaactcc tggctggcgt cgccgaggct ttcggcctgg atcccgctgt
     3541 tgcggaagcc cggatgcagc gagcccttga ggtgtgcagg gagataaggc gccatggtct
     3601 tcaggcacac gtgacccccc attcactcta ctcgctctct ctccccctgt cacagctgat
     3661 caaggaacat ataccggctg attcaattat ctcactgcat ttcctggaat cggatgatga
     3721 gcgcaggatg gcaaagggcc acgcagatgc agcccttgac ctctccggcc tcgcctcccg
     3781 gctgctgctg gtacacaaca cggttatcac cagggatgaa gcggtgaaga cagcctctgc
     3841 cgggaacaca tggttctgcc tctgcccctc gtcaaacatc catatatccg gcaagatgcc
     3901 acccgctgca atgctgaggg aggtgacccg gaggattgca gtgggaactg acagtcttgc
     3961 atctacagac cggttgagca tgatcaggga gttgcttctc ctgcaggagg cagtgccggg
     4021 catcacggca gaggagctga taagatgtgg aacgatcaac ggggccatgg cgcttaagtg
     4081 tgatgacacc ctcggatcaa tagaacccgg caagcgtccg ggcctgcttc tggtggaacc
     4141 gtttgacctg cagaatatgc gtctgcttcc tgagagccgg gtgcggagac tggtatgacg
     4201 cttacgtctg cttcgggaaa tcctcgtgag aaaactgcta tggnnnnnnn nnnctccggt
     4261 aatcgccggc aagaatccac ccctggcagg catctcttct cctgagagcc gcgtgcggag
     4321 actgacatac cacctgcctt tacttccggg cagccggata catatattat cgtaaatttg
     4381 tgccctgatc tatcatttgc gtaatgccca ctttcctttt tgacagcatc gtgttcggac
     4441 cggtgcacag ccgccggctg ggcgtctccc tcgggataaa cctccttccc gtcacacgca
     4501 aggtgtgctc atataattgt atatactgcg agtgcggctg gacaccggac aaaccggact
     4561 caaaggaaaa gctcccgtcg cgccgccaag tccgtgatgc actgagaaag aaactttccg
     4621 aaatgaagga gcagggcaag gcgcccgatg ttatcacata tgccggaaac ggcgaaccta
     4681 cactgcaccc cgacttcgcc gggataatag atgattccct ggcactgcgc gatgagttct
     4741 tccccgcggc aaaggtgtca gtgctctcca acggctccat gctccaccgg gaaagcgttc
     4801 gcgaagcact gaaaagagtt gaccagaaca tgctcaagct tgattcggta tttcctgata
     4861 ctgtcaggca gattaaccag ccccatgtga aaactgacct tgccaggtat gaagagtata
     4921 tcagggagct tgagggcagg ttcatcatcc agaccctctt cgtcaggggt gagtatgagg
     4981 gccggacggt tgacaacacc acagaggaag agatcactgc atggcttgaa gtcatatcga
     5041 ggctcaggcc tcaactggtg atgatataca ccatacaccg tgatacgcca ctgggcagca
     5101 atctcaggaa agtgcctgaa agcgaactca atgccatcgc ccaaagggtc aaccggctgg
     5161 gcatcgccac aagcgtctca ggataacagc catgaacggg atgagagtcc tgataagccc
     5221 tctcagctgg ggtttcggcc atgccggaag gatgatacct ctggcactgg cattgaaacg
     5281 aagaggctgt gaggtgatct tcgcagccga tgcactcctg accaaaatga tcgcaccgga
     5341 gctgcctgac atcaaaacag ttgtaatccc gggcctcacg atcaggtact cccgtttcat
     5401 gccgcagtac ctcaccatct tcctgcaatt gcccgtgata gttgcatcgg cggtaaggga
     5461 acatgccacc ctccgcagac tggcgaaaga gcttaacccg atggtgatta tctctgataa
     5521 ccggttcggc ttttaccaca aaaaaatctt ttcagtctat atcacccacc aggtgaggat
     5581 tgccttcccc gcgttccttg cgtttctgga accgcttgcc gcatggatgc accggatgat
     5641 aatcagcagg tatgatctct gtctggtccc tgattatcgc ggcagcgaaa acctgtcggg
     5701 aagactttct catggtggca ggctcccggc aaatgttgca tatatcgggc cactgtcaag
     5761 atttggcgtt gccggcagtg aaccatcttc gcccgcccgg gcgaagagtg taccacccgg
     5821 acccggccgt gcgaagggcg aaacagttgc gcccggtcag atgaagcgca tgccatttgc
     5881 agccaaccgg acgaaaagcg aaccagttgc gcccggccag atgaaaggtg agccattgca
     5941 gaccggccag acagagaata atccattgca accaggcgga ggggtgcctc ccgcaccccc
     6001 ggcacaaccc tacgtctgcc ttattctgtc aggtcctgag ccacagaggt cactgctgct
     6061 ccggaaggtt tctgaatcac tgcgggagtt tccgctgaca gtgctgagtg ccacacctcc
     6121 acaagcacct acacctccgg aaatacctac acctcagcaa gcacctacac ttcctgaagc
     6181 actcacacct ccgaaaacgc ctccgcctcc cggtcaggca atacatttaa taaccggagc
     6241 tgatacaacc acaatgcgac aggtcattac cggggcatca atggtgattg caagagccgg
     6301 atatacttca gtgatggagc ttgtttcact tggaaaggga gcggtgctta tacccacccc
     6361 gggacagacg gaacagcagt atcttggcag gcaccttgac ggacgttacg gttttatcac
     6421 catgaatcaa aacaggcttg aaaggctggc ctcagtgatc ctacctgagc ataaaaaaga
     6481 gtgagagacc catcatcaca gccccggctg ccactcctgt ccagacctcc gtgggcgtat
     6541 gatcaccgag gtatattctt gatgacatca ctgctccggc tatcagcgcc actgctacag
     6601 actgccagag catgccgatg ttgtacactg tcgtcatgca gataaccagc accaccaatc
     6661 ccccgattcc tgaagcatgc agtgagattc tgtaaaagat agttatcaga agtatggtga
     6721 aagtgacaat tgctattgag atgaaaaaag ccctgaacag agtggggacc tgcatcctca
     6781 acagaatgaa agctgtcagg ctgtacatca taagtacaaa tgaaagcagt aataccctct
     6841 cattgcggtc actggcattg aatgttctga tggagcctcg tgcataaagg aaagtggcga
     6901 aggccattgg caaaagcagg ttattggcca tcacaagtat taaaatcatc ctcttgatct
     6961 gggagggaag aaatgagtgc agtgtgtgtg cgctgtaaat gatcagcagt ccgtacagcg
     7021 gcatgaatat cgggtgaaaa acaactgaaa taatttcagc cgctctcttc agccgccgtt
     7081 cagagcccgg gttcatcctg ctctcctcct cagccatgcc attgtcaggc atttcattct
     7141 ctccgggctt catgttatct ccttcctcat tcttgcaaca ggtatattca gctgctccct
     7201 gtacttcgcc acggtacggc gggcaatctg atagcccttc tcctgcagta ttttcgtcag
     7261 cttctcgtcc gtcagcggct tgctcttatc ctcattattt atgcactcct gcagtatcct
     7321 ctttatctca cgggttgaca cctcatcacc ggtatcggtc tgcatcccct cggagaagaa
     7381 gaatttcagc gggaaaatgc cgaaatgtgt ctgtatatac ttgctgttcg ccacgcgtga
     7441 caccgttgat atatcaagcc ctgtcatatt ggccacatcc ttgagtatca taggcttgag
     7501 tttggtgtca tcgccttcgg cgaaataatc ctcctgatac gcaagaatag cattcattgt
     7561 cagcagcagt gtgttctgcc tctgtctcat tgcatcgatg aaccacctgg cagcatcaag
     7621 cttctgcttc acgaacatga ttgcgtcacg gttctgcttg ccagccttct tgtcacgggt
     7681 gtatgactca agcatctcac tgtaatcacg gttgaccctc agttcaggga tgttcctgtt
     7741 gttgaggctg agctcgaaac cgctgtcact ctgttcaagg atgaagtcag ggattatatg
     7801 gtgggtcagc ctggcatacg ggtcgctcat ggtgccgccg ggcttgggat tgagttttag
     7861 cacctcgtca atgatacctt tcatgtcact ctcctcaatc cccatcctgt tcatgatctt
     7921 ttcatagtgc cgtctggaaa actcttcgaa ataatcggta agaacagttt tggcaagggc
     7981 aactgagggg acagactggc tcttcctctc tatctgaagc aacaggcatt cacgcaggtt
     8041 gcgggcgccg acacctgccg gttccagacc ctgtattatc ttcagtatcg catccagctc
     8101 gctgacatcc gtggtaatgt tctggagaaa tgcaaggtca tcaaccatat tctccacctc
     8161 gcggcggagg tagccatcgt tgtctatgtt tccgattaca tactcgccaa gaagtttttc
     8221 cctctcggtc tctgtacgga ggttgaactg ctgtatcagg ttttcgcgga atgatgatcc
     8281 ggcagcatag ggtatatctt cacgccgttc atcatctttc gatgtgttgt tgacctgaag
     8341 cctgtaatca gggatatcct cgtcattcag gtaatcatcg agagaaaact catcctgttc
     8401 acgttcttcc tgttcgtgat caacctcctc ttcatcctca tcgccatgta cctcatcctc
     8461 ttcatgcccc tcttccaggg cgggattctc ctccagctcc ttctttatcc gttcctccat
     8521 ctgcatggca ggcacctcca gcagcttaat catctggatt tgctgcgggg agagcttttg
     8581 tagcagtttc tgctggagtt tctggtcaag catggagtcg tttattcact gttttaaaat
     8641 tcagcgtttc gtggagtacg tgggaacggt atcacatcgc ggatgtttga cataccggtc
     8701 atgaagagaa taagcctctc aaagccaagg ccaaagcccg aatgcggtac agtgccaaat
     8761 cttctggtat caaggtacca ccacaaatcc ttcagcggca ggttcagctc ctcaatcctt
     8821 cgcgtcagct tatccagctc ctcctccctc tgcgaaccgc ctattatctc gccgatgccg
     8881 gggaagagca catccatggc cctgactgtt ttaccgtcat cattctgctt catataaaaa
     8941 gccttgatgc tcttcgggta atctgtaagg atcaccggtc tcctgaagtg cgtctctaca
     9001 agataacgct catgttctgc ctgaaggtcg gagccccagc taacgggata ctcccacttg
     9061 cggccggcat cctcaaggat cctgacagcc tctgtataag tcagtctcat atagctgttg
     9121 ccaagcacga actcaagcct ttcaatcagg cccttgtcaa tcatcttgtt gagaaattcc
     9181 aggtcgtcac ggcagttgtc aagagcatat ctgatgaggt actttgtaaa atcctcagcg
     9241 aggtccatat tgtcatttat atcccagaag gccatctccg gctcaatcat ccagaactcg
     9301 gccaggtgtc gcggggtatt cgagttctca gcgcgaaatg taggaccaaa agtgtatatc
     9361 tcgcccagtg ccagggcacc aagctcaccc tcaagctgac cggagacagt aaggttgacc
     9421 ggcttcccga agaaatcctg ttcataatct actgtaccat cctttttgcg gggcaggttt
     9481 tcaaggtcaa gggttgtcac atggaacatc tcgccggcgc cttcggcgtc acttcctgtt
     9541 ataatgggtg tatgcaggta atagaatccc ctgtcattga aatacttgtt aattgcataa
     9601 gccatggcgt ggcggatgcg catcactgct ccgaaggtgt tcgtgcggaa gcgcagatgg
     9661 gctatctccc tgaggaactc cattgaatgg ccctttttct gcagcggata ggtttccgga
     9721 tcacatttgc cataaatggt gatatcagat gcgttgatct ccacgttctg acctttgccc
     9781 tgcgacggaa ccagggtgcc ggtcacagcc atgcttgcgc cggtggtaat ctctttcagc
     9841 agctcagcag ggaaactatt tatttcaatg acaatctgaa tgttgttaat tgttgaaccg
     9901 tcattaagcg caacaaatgc cacattcttt atctcccgct ttgtcctgac ccagcctttg
     9961 accagcactt tttctcctgt cgggtgaccg gcaagcagtt cctttacttt ctttctcatt
    10021 ttaaatgatc atcgcattat gaaggcaaaa ataatatttt gttcctgttg ccatactgat
    10081 attcgcggaa atggcactgt ttttgtgcat tgacgcaatt tttcacctgt tggcggcgtt
    10141 ttttaagcgt accggtatat cttgacttaa tcaggtaaaa agggtaattt tactgttcaa
    10201 actgtaaacg tttatattat ggaagtcgac agcaagatca ttgtggtgcc atgggatttt
    10261 accccggtga cagaatatgc attgcagcat gccatcaaga tttcactcat gacacgaaat
    10321 ccgatatgcc ttctgcatat cgttgagaag atcaccaccg agggagggat gaaggccaaa
    10381 caggctgcca tgaataacag ggggagtgat gttgagcgca tgtttggcgt aaaggttaag
    10441 actcacgtta ccaggggtac cattttcaaa cagattccgg agtttgtcaa tgaccgcaat
    10501 gcatcgctgg tggttatggg tacgcacggt atgagcggta tgcagaaggt taccggcagt
    10561 tgggcgctga aagtcattgc caagtcgaag gtgccatata ttgtggtcca ggccccgccg
    10621 gccgataatg aaaagtatca tgacattgtg ttcccggtcg atttccgtgc agaggagaag
    10681 gagaagctct cgatggccat cttcatggga aaatacttcg actccaagat acacatcctt
    10741 aaagccatca ggaaggatga ctcactggcc aaaaggacca acctgaacat gaactatact
    10801 gtcaggatgc tcatccagaa caatatagag tatgagatcc gcgaggaacc ggctgacaag
    10861 cttggcgaac gggctcttga gtttgcccag gagatcaacg cagacctgat actgatcatg
    10921 accaccaagg atatcacttt tgccgactat atctggggcg ctaaggagca atacatcatc
    10981 gccaacagtt cgaagatacc tgtctgctgt gtgaacccaa cctcggcatt tgccaatctc
    11041 ggccagttca tgtatggtta agacccggta agcgttagta ctcaatgtct attgttgtca
    11101 gcaacgttac gaaatattac ggagctcaga aggctcttga tgatgtttca tttaccatcc
    11161 cggcaggtga gattgtaggt ttcatcggcc ccaacggagc aggaaagtcg acgatgatga
    11221 agatcattac cggcttcttt cccgcagacg cgggagaggt taccgtatgc gggcttcagg
    11281 ccggcccgtc tgccagggag atgaggcggc agataggtta cctgcccgag aacaaccctc
    11341 tctaccttga tatgtatgtg aaggagtacc tcggatacgt tgctgaaatt tattcgcctg
    11401 gcagaaagag ccgcgaggct gtcagttccg taatagagcg caccggactt accccggaac
    11461 aggggaagaa gataggcgcg ctttccaagg gttaccgcca gcgggtggga ctggcacagg
    11521 cgctgatcca tgatccgaag gtgctgatcc tcgacgaggc caccacgggt ctcgatccca
    11581 accagatagt agagatacgc aacctcatct cagaagccgg caaagaaaag accgttatgc
    11641 tgtcaacgca tatcatgcag gaggtggaag ccatctgcag cagggtgata ataatagacc
    11701 atggccgtat tgtggctgac ggccccattg acgagatctc ctcgctcgcc ggcgcccgcc
    11761 ttgaggaaac cttcaggcag ctgaccggaa gatagcctgc agcccccaga aattatcatt
    11821 tcattggtta tttgcagaac agagcatcag tggctttgtc aatgttattt ggagaacgtc
    11881 taaattagtt ccctgtttaa aaatattctg aaatttcatg tcacaaaaca gaggattgtg
    11941 acacgtactg atgaatgatg ctcctgcaaa ggaaagtatc catctctgat tgtgtggaat
    12001 aataggagaa ttgaatgtaa gtgtttgatt taataacttt tagctgcaaa c
//
LOCUS       DAOPBK010000074        11919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5313_length_11919_cov_4.488031, whole genome shotgun sequence.
ACCESSION   DAOPBK010000074 DAOPBK010000000
VERSION     DAOPBK010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5313_length_11919_cov_4.488031"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..2039)
                     /locus_tag="PLA17_05820"
     CDS             complement(324..2039)
                     /locus_tag="PLA17_05820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPK84966.1"
                     /translation="MNKRTLLLTIFITATTVIGSQPSGNVSFVSPLRETPSLSASFAE
                     LRADHFHSGLDYRTGGVQGKEVLAIDEGYVYRIAVSPTGFGKALYVRHPSGYSSVYAH
                     LRSFRPDIEEYVRQQQYSRKSFSVSLFPQRTRFTVSRGEVIAWSGNTGGSTGPHLHFE
                     IRDSASEDPVNPLTFGLGISDRMRPVIDRVILYPLTRGAAVNSSHGNLSMRTVPSDGS
                     YGIAATTVPVVYGPVGIGIKCWDIFDNSSNKCGVYTIELLADGLKIFSFRADRFSYSE
                     TRYINAHIDYAARATSGEYLHRLHIQPGDRLSMYDGHVGRGVLKFNDDAEHEIKIIVT
                     DVHNNRSWVTFRVRSVSNPPVPPAEIKCSKVIPWGKAADFTADGIRIHFPAGALYDTL
                     WFNYKTRENTQRFLSPVHMLHNETVAIHDRFRLSVRPDTIIAGLENKLCLARISSKGV
                     VSYAGGKFAYGFVSGEMNSLGDYAVTLDTIPPSAKFSFANGANLSGRSQFTATIRDEF
                     SGINSYEMYVDGEWILAEYDAKNNLLICRPGTSEMKPGMLHKIELRVTDNCGNSNTVT
                     SEFRW"
     gene            2150..2443
                     /locus_tag="PLA17_05825"
     CDS             2150..2443
                     /locus_tag="PLA17_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84967.1"
                     /translation="MSVQGYEEYLLLRSSVEALIAEHDRLVEMAAQLNNKLSEAERQL
                     AAKEEEVKELKSRYERAKFSGAILGSGEDAVTARRRVSELVREIDKCIALLDR"
     gene            2454..2744
                     /locus_tag="PLA17_05830"
     CDS             2454..2744
                     /locus_tag="PLA17_05830"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPK84968.1"
                     /translation="MDDKLSIRVNVADRYYPLKVESESEERIRKAARMINEKVLQYKQ
                     RYIDKDTQDFLAMAALQYVIRLTELEEMEEDSSLEDGLKELTEKINLCLSKR"
     gene            2992..4542
                     /gene="rny"
                     /locus_tag="PLA17_05835"
     CDS             2992..4542
                     /gene="rny"
                     /locus_tag="PLA17_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPK84969.1"
                     /translation="MIIATTGVCFAIGGAALIAGSVLSFYLWQVMLRNRKKRIIADAE
                     TEAEVIKKEKILQAKERFLQLKAEHEKVINERNNRINQAENRVKQKELTLSQKLEEVQ
                     RRKNEADAIRDNMTQQIELLEKKSEEVSKLHRQKVEQLEAVSGLSADEAKNQLIDSLR
                     EEARTNAMSYINEILDDAKMTATREAKRIVLMAIQRVAAETAIENAVTVFHIDSDEIK
                     GRIIGREGRNIRALEAATGVEIIVDDTPEAIVLSGFDPVRREIARLALHQLVTDGRIH
                     PARIEEIVEKTRKQVEEEIVETGKRTAIDLGIHGLHPELIRLVGKMKYRSSYGQNLLQ
                     HSREVANLASIMAAELGLNPKLAKRAGLLHDIGKVPDDEPELPHAVLGMKIAEKFKEK
                     PDVINAIGSHHDETEMTTLIAPIVQVCDAISGARPGARREVIESYIKRLNELESVALS
                     YPGVTKTYAIQAGRELRVIVGADKVSDQDTESLSFEIARKIQNEMTYPGQIKITVIRE
                     TRAVNYAK"
     gene            4542..5702
                     /gene="wecB"
                     /locus_tag="PLA17_05840"
     CDS             4542..5702
                     /gene="wecB"
                     /locus_tag="PLA17_05840"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPK84970.1"
                     /translation="MKKLKILNIVGARPQIIKASAISRAIAGEFSDEIEEVVVHTGQH
                     YDRELSDVFFDELEIRQPDHNLQVGSGRHGRQTGMIMERIEDVILDEKPDCVVIYGDT
                     NSTLAAALAAAKQHFPVVHIEAGMRSFNKTMPEELNRIMTDHASTLLIAPTNTAFRNL
                     MSEGFHPENVAPYSINNPRIFLTGDIMYDNSLFYADLAERKKHSYLTGLGIDAREYVL
                     VTIHRDSNTDNPERLSAIFSTLKELSEANSMPMIMPLHPRTMIALRNRVPELYAAISD
                     HPFMMVIPPVSFLEMILLEKRARLIITDSGGVQKESHFFSRPCIVLRKETEWVELVRN
                     GTVKLADASPARIKDAWDYFIANSSTLQYPAFYGDGKAAIVILKEILAVFRS"
     gene            complement(5692..6555)
                     /locus_tag="PLA17_05845"
     CDS             complement(5692..6555)
                     /locus_tag="PLA17_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPK84971.1"
                     /translation="MKKTELIINPDGSVFHLHLLPGDIAEKIILVGDPGRVEMIAEML
                     DSVRVRKINREFCTVTGSYGETEVTIISSGIGTDNIDIVINELDALANIDLGTGMTLA
                     EPRSLTFVRIGTTGGLQPELPVGSFIATAKAVGFDGVLHFYEGYEWCLDHLLADSLAS
                     HLEWPDTLAYPYAVDADAGLLEKLAAGTARGITISTPGFYAPQGRRLRLEPFDNKLND
                     NITSFRFKGEKITNYEMESSAIYGLSKLLDHKALTLCVVIGNRVTGEFLTDYKPAVRK
                     LAAQVLDRLSS"
     gene            6822..9308
                     /locus_tag="PLA17_05850"
     CDS             6822..9308
                     /locus_tag="PLA17_05850"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPK84972.1"
                     /translation="MTYRTGRIIFPAILSIILSLNSVTGASQAKKRTEALRITEPIQI
                     DGKLDEAPWQKASPAGDFIIYNPHNGMPSKFRTEVRLLYDDDALYIGAIMYDSYPDSI
                     FTQLGQRDAGDINADHFWIEISPFNDGLNGEMFKVSASNVQIDNKMSTADSWMHRDSW
                     DAVWHSNTSISDEGWIAEMKIPYSALRFPRSASQLWGINFWREVRRNRETSSWNFVTK
                     EFGSPISHMGELTGIRDVKPPLRLSLVPYVSGYLEHVTDESGVGTSYNGGLDLKIGLT
                     ESFTLDATLIPDFGQVQSDDHVLNLSPYEVRYNEKRPFFMEGTELFNKGGIFYSRRIG
                     GTPHLIRDVYGMVGENETVIRNPAEVSLLNATKISGRTSSGLGIGLFNAVTNTTWAVV
                     ENKLTGETRHIMTEPLTNYNMLVLDQTLRNDSYVSVMNTNVIRAGKRNENFYTANVSG
                     FEALVKTADRLWSVSAGAVLSQKYYSSDPTALGHSLNFNAGRTGGKFRADYRFSMMSD
                     TYDPNDMGYLRKNNEIEHSVDFSYNTYEPFGNIMTTRTSLDFSYGQLYNPRAFTTAEV
                     RLDAMILLMNFWSLSLDLELTPWGEDDYFEPRTPDMSMFYHRPAAFEASFRGDTDRSK
                     RFYIQGNAEYMKAWSDYGQYGYEWSLQPEFKVGRRFSFSYSISVEDLINDIGFVPGNP
                     HEGITFGKRDVTTITNTISGAFIFSANSYLTLRGRHYWSRADYDGDYFTLQPDGSLLD
                     RIYSTPLNNSDVNTNFLNIDVVYTWRFAPGSELSLVWKNSIWSEGDVIIRNGFDNFGD
                     LLSMPQTNSLSLKILYYLDYQNFRKIFKSK"
     gene            9321..9776
                     /locus_tag="PLA17_05855"
     CDS             9321..9776
                     /locus_tag="PLA17_05855"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HPK84973.1"
                     /translation="MIQRIQTLFLLAVVFLSSLMLTGDLIQLTTDGGALFSISFPGMK
                     NQAGEVVQRLWPMSVILALVPLLALIALFLYKKRPLQMRLTMLVLLLSLGTLILGAFY
                     VLMFDRKIDVNIIWKVKVLFPFISAILAWLAYRAILKDELRVRSYDRLR"
     gene            complement(9833..10291)
                     /gene="pyrI"
                     /locus_tag="PLA17_05860"
     CDS             complement(9833..10291)
                     /gene="pyrI"
                     /locus_tag="PLA17_05860"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HPK84974.1"
                     /translation="MNDKKQLLVSAIESGTVIDHIPASSLFKVIKILGLDRIRNQITF
                     GTNLESKTSGKKAIIKIAGKFFEDDDIDRIALVAPKAKLNIIRDYQVIEKRVVEVPDT
                     INAIAKCMNPKCITNLEKVTTHFRVVSKKPVALKCLYCEKITDQERLEII"
     gene            complement(10307..11215)
                     /gene="pyrB"
                     /locus_tag="PLA17_05865"
     CDS             complement(10307..11215)
                     /gene="pyrB"
                     /locus_tag="PLA17_05865"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPK84975.1"
                     /translation="MKSRSLVSIDDLTTEEILKILTLAAEFEKNPVQDILRGKVVATL
                     FFEPSTRTRLSFESAVNKLGGKVIGFTDSSNSSVAKGESLNDTIRTVNNYVDLIVMRH
                     PIEGSARYASEIATVPVINAGDGANQHPTQTLLDLYSIQKTQGTLDNLNICMVGDLKY
                     GRTVHSLLMAMSRWKTSFHFVAPEELKMPDEQKLYLRNLGLDYHETTDLNEVINMADI
                     VYMTRVQRERFSDPMEYEKVKNAYVLHDYMLSDTKPEMRILHPLPRVNEISTDVDGNT
                     KAYYFEQALNGVYTRQAIICLLLGLK"
     gene            complement(11313..>11919)
                     /locus_tag="PLA17_05870"
     CDS             complement(11313..>11919)
                     /locus_tag="PLA17_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPK84976.1"
                     /translation="DTYKTIEGESQGLFKDRGSRFIALALPVTSREEIKARLAELRKE
                     YHDARHHCFAWVLGPDRQAWRVSDDGEPSGTAGRPILAQINARELTNVLIVVIRYFGG
                     TLLGVGGLINAYRNAAADALDKATMVEKYIMVRWSVSFPYTSMNDVMRVLRDESCEQS
                     EHRYSGTDVTVEIRFRASMADHVTGRLNKISGLQMKWLATE"
BASE COUNT         3102 a         2810 c         2958 g         3049 t
ORIGIN      
        1 tatacataag tgtttgtgta tgcaatacat gtgggtttca tggttataaa tcagtttaat
       61 gaacaagcta ttaaaaggat gctttatcaa attgaatatt aactttcttg tttagtgcta
      121 agttaataaa aaagtgactt ctatatactt aatcttctca caggcttttt tgcccttact
      181 cctgaaaatc acgggataat cccaatgcag ggtctcaaat ccctcactgc ctgtatgaca
      241 ggataatccg cataaatata atgtaacgag gggtctgcag acccctcgct acatgcatgg
      301 cagggtaatc cgtacatata tgattaccac ctgaattctg atgtcacggt attgctgttg
      361 ccacaattgt cggtaaccct cagttcaatt ttgtgcagca tgccaggttt catttcagag
      421 gtcccgggtc tgcatatgag caggttattc ttggcatcat actcagccag tatccattct
      481 ccgtcaacat acatctcgta gctgttgatt cctgagaact catcccgtat tgttgcagtg
      541 aactgcgacc ttccggaaag gttggcgcca ttggcaaatg agaactttgc agatggtgga
      601 atggtatcaa gggttacggc ataatctcca aggctgttca tctctccgct cacgaagccg
      661 taggcaaatt ttcctcctgc atatgagaca actcccttgc tgctgatcct ggccaggcac
      721 agcttgttct caagacctgc aattatcgtg tcaggcctta ctgacagcct gaacctgtca
      781 tgaatggcta ctgtctcatt atgaagcata tggacagggg acaggaatct ttgggtattc
      841 tcccgggttt tatagttgaa ccacagcgta tcatacagcg cccctgcagg gaaatgaatc
      901 cttatcccgt cagcggtgaa atcagcagcc ttgccccatg gaatcacctt gctgcactta
      961 atctctgccg gaggaaccgg gggatttgat accgacctga cccggaaggt gacccatgac
     1021 ctgttattat gcacgtcagt aacgatgatt tttatctcat gttcagcgtc atcattgaat
     1081 ttcaggactc ctcgtcctac atggccgtca tacattgaaa gcctgtctcc cggctgtata
     1141 tgcagcctgt gcaggtattc gcccgaagtg gcccgtgcag cataatcaat atgtgcgttg
     1201 atataccttg tctcgctgta agagaaacgg tcagccctga agctgaatat tttcaatccg
     1261 tcagccagca gttcaattgt atatacacca catttatttg agctgttgtc aaagatatcc
     1321 cagcacttga ttccgatgcc cacagggccg taaacaaccg gtacggttgt ggcagcgatg
     1381 ccgtacgatc cgtctgacgg cacggtacgc attgagaggt tgccatggct gctgttgacg
     1441 gcagcacccc ttgtcagcgg gtagaggatc accctgtcaa taaccggccg catacggtca
     1501 cttatgccaa ggccaaaggt gagcggattg acggggtctt cagaagctga gtcacgtatc
     1561 tcaaaatgca gatgcggccc tgtagagcct cccgtgttgc ctgaccatgc tatcacctct
     1621 ccgcgactaa cggtgaaccg ggttcgctgg ggaaagagag agacggaaaa gctcttgcgg
     1681 ctgtattgct gctgcctcac atactcctcg atgtcaggcc tgaagctcct cagatgtgca
     1741 tagaccgacg aatagcctga cgggtgcctg acatagaggg ccttgccaaa accggtggga
     1801 ctgactgcaa tacggtagac atagccttca tcaattgcaa gcacctcttt gccctgaact
     1861 cccccggtac ggtaatccag tccggaatga aaatggtcgg ctctcagttc tgcaaagctg
     1921 gccgagagcg aaggagtttc cctgaggggg cttacgaatg agacatttcc ggaaggctgt
     1981 gatccaatga cagttgtggc tgttataaaa atggtaagga gaagtgttct tttgttcatg
     2041 tcggtgtcag ttctgcaaat ctattgttct gcccgtgaag agcaaactta attccgcttt
     2101 ttttgaaaag agcgaaaata gtattatttt tgtattagag ttgtcagcca tgagtgtaca
     2161 gggatatgag gagtatcttt tgctcaggag ttcagtggaa gcgctgattg ccgagcatga
     2221 caggctggtt gagatggcgg cgcagctgaa taacaaattg tcagaagcgg agaggcaact
     2281 ggcagcgaag gaagaagaag taaaggagct taagtcaaga tacgaaaggg ccaagttctc
     2341 gggagcaatc ctggggagtg gagaggatgc cgttacggca cggaggaggg tgagtgaact
     2401 tgtgcgggag atagataaat gtattgctct tttagacaga taaatatata gcaatggatg
     2461 ataagctatc gatccgggtt aatgtggccg acaggtatta tccgttgaag gtggaaagtg
     2521 agagtgagga acgtatccgc aaggcggcaa ggatgatcaa tgagaaggtg ttgcaataca
     2581 agcagcgcta cattgacaag gacacgcagg attttctggc gatggcggcc ctccagtacg
     2641 tgatcaggct cactgagttg gaagaaatgg aggaggattc atcacttgag gatgggttga
     2701 aggagttgac tgaaaagatc aatctctgtc tgtcaaaaag atagatacgc gttctttaaa
     2761 atagcaggat attgcccgca ttgtttctct attcgttttg gaaactcaac agtttttact
     2821 ttggagatgg tcttggattg cttaggacag gcctcacccg gttcgccggg aacccgcttc
     2881 ggcggggcag tggaagcccg aggcaagacg gtcgctccac ccatgtagat atggggtttt
     2941 taaaagttgg aggaactctg cgggtttttt tatacaaata acaatagtta tatgatcata
     3001 gcaacaacgg gggtctgttt cgccatcggc ggggcagccc ttatagcagg atcagtgttg
     3061 tcattctacc tatggcaagt gatgctgcgc aacaggaaga agagaataat tgccgatgca
     3121 gaaactgaag ccgaagttat aaaaaaggag aagattctcc aggccaagga acggttcctg
     3181 cagcttaagg cagaacatga gaaagtaatc aacgagcgta acaaccgcat taaccaggca
     3241 gagaacaggg tcaagcagaa agagcttaca ctgtcacaga aactggaaga ggttcagcgc
     3301 aggaagaatg aggctgacgc catccgcgat aacatgacac agcagattga gctgcttgag
     3361 aagaaatctg aggaagtatc aaaactgcac cgtcagaagg tggaacagct tgaggcagta
     3421 tcgggcctct ctgcagatga agccaagaat cagctcattg attcactcag ggaggaagcc
     3481 cgcacaaacg ccatgtctta cataaatgaa atccttgatg atgcgaagat gactgccacc
     3541 agggaggcca agcgtattgt gcttatggcc atacagcgtg ttgcagcaga gacggcgatt
     3601 gaaaatgccg tcaccgtatt ccatattgac tcggatgaga tcaagggtcg catcattggc
     3661 cgtgagggca ggaatatcag ggctcttgag gctgcaaccg gagtggagat aatagttgat
     3721 gacacacccg aggcaatagt cctctccgga tttgaccctg tcaggcgtga gatagcgcgc
     3781 ctggcactgc atcagctggt caccgatggc cgcatacacc ctgcacgtat cgaggagata
     3841 gtagagaaga cacgcaagca ggttgaggaa gagatagtgg aaacgggcaa acgcacggca
     3901 atagaccttg ggattcatgg cctccatccg gagctcatca ggctggtggg aaagatgaaa
     3961 taccgttcat catacggaca gaatctcctg cagcattccc gtgaggtggc caatctggct
     4021 tcaataatgg cagccgagct tggccttaac ccgaagcttg caaagagagc aggactgctg
     4081 catgatattg gcaaggttcc cgatgatgag ccggaactgc cgcatgctgt ccttggaatg
     4141 aagatagcag aaaaattcaa ggagaagcct gatgtgataa acgccatcgg gtcacatcat
     4201 gacgagacgg agatgaccac actcatcgca ccgatagtgc aggtatgtga cgccatttca
     4261 ggtgcccgcc cgggcgcacg gcgcgaagta atagagtctt acatcaaaag gctcaacgaa
     4321 cttgagtcgg ttgccctctc ttatcccggt gtgacaaaga catatgcaat ccaggccgga
     4381 agagaactga gggtcatagt aggagctgac aaggtgtcgg atcaggatac cgagagcctc
     4441 tctttcgaga ttgcaagaaa gattcagaac gagatgacct atccgggtca gatcaagata
     4501 accgttatca gggagacaag ggcagtcaac tacgccaaat aatgaaaaaa ctcaagatcc
     4561 ttaacatcgt cggcgcgagg cctcagatca taaaagcctc cgccatcagc cgtgcgatag
     4621 cgggagagtt ctcagatgag attgaggagg tggtggttca taccggtcag cattatgaca
     4681 gggaactctc tgacgtcttt tttgacgaac tggaaataag gcagcctgat cacaatctgc
     4741 aggtagggtc aggaaggcac ggcagacaga ccggaatgat tatggaacgc attgaggatg
     4801 tgattcttga tgagaaaccc gattgtgtgg ttatctacgg tgacacaaac tctaccctgg
     4861 cggcagcgct ggcagcagcc aagcagcatt tcccggttgt ccacatagaa gcaggcatga
     4921 gatcattcaa caagaccatg cccgaggaac tcaaccgcat catgactgac catgcctcaa
     4981 cacttctgat agctcctaca aacacagcct tcaggaacct catgtctgag ggcttccacc
     5041 cggagaacgt ggctccctat tcaatcaaca accccaggat cttcctgaca ggtgacatca
     5101 tgtatgacaa ttctctcttt tatgctgacc tggcagagag gaaaaaacac agttacctga
     5161 cagggctggg gatagatgcc cgggaatatg tactggttac catccaccgt gattcgaaca
     5221 cggataatcc ggagagacta agcgccatat tcagcacttt gaaggagttg tctgaggcaa
     5281 actcaatgcc catgatcatg ccgctccacc cgagaacaat gattgctttg cgcaaccgtg
     5341 ttccggagct gtatgcagct atatccgatc accccttcat gatggtcatt ccgccggtct
     5401 ctttccttga aatgatattg ctggaaaaga gggcgcgact tatcatcacc gattcaggag
     5461 gtgtgcagaa ggagagtcat ttcttctcac gtccatgcat cgtgctcagg aaggagactg
     5521 aatgggtgga acttgtcaga aacggaacag ttaagctggc agatgccagt ccggccagga
     5581 taaaagatgc ctgggattat ttcatagcca acagcagtac actgcagtac ccggcgttct
     5641 acggagacgg aaaagcggca attgtaattc tgaaagagat acttgccgta ttcaggagct
     5701 gagtcggtca agaacctgtg cggcaagttt tctgactgcc ggtttgtagt cagtgagaaa
     5761 ttcaccggtc actctgtttc ctattaccac gcagagtgtc aatgccttgt gatccaatag
     5821 ctttgatagt ccataaatgg ctgagctctc catctcgtag ttggtgattt tctctccctt
     5881 gaacctgaat gatgtgatgt tgtcattaag cttattgtca aatggttcaa gcctgagccg
     5941 gcggccctgc ggtgcataga agccaggtgt cgatatcgtg atccccctgg ctgttccggc
     6001 agccagtttc tcaagcagtc cggcatctgc atccacggca taggggtagg caagggtatc
     6061 aggccattca aggtgcgatg caagtgagtc agccagcaga tggtcaagac accattcata
     6121 tccctcataa aaatgcagaa caccatcaaa tccaactgct ttggcggtgg ctatgaagga
     6181 acccacgggc agttccggct gaagtccgcc agtagtgccg atccttacaa atgtcagtga
     6241 acggggttct gccaaagtca ttccggttcc cagatcaata ttcgccagtg cgtcaagctc
     6301 gttgataacg atatcgatat tgtcagtgcc tattcctgat gatatgatag tgacctcagt
     6361 ttcaccatag ctgccggtga ctgtacagaa ctccctgttg atcttgcgta ccctcacgct
     6421 gtcaagcatt tcagcaatca tctcaaccct tccgggatct ccaacaagaa ttatcttttc
     6481 ggcaatgtcg ccgggaagaa gatgaaggtg aaacactgag ccgtcaggat tgattataag
     6541 ttctgtcttt ttcatcacga tatagttttg attctaaggg tgtgatggaa cccatataaa
     6601 tgaaaagtat ataaacagat gtttgtctgt ttgacagatg tgggtttcat cgcacgacta
     6661 ataagctgta aatgtacaaa aaatgtcgac tgtaaatcaa tagcatggca gatgatgccg
     6721 gcagttaata attgttaatt tgacaattgc atcaccatga tttgcggcag gaatttatct
     6781 ttgctgcctg accgaaacag atgaaaatgc attttcccca tatgacatac agaaccggca
     6841 ggattatttt tccggcaata ctttcaatca ttctgagtct taacagtgtg acaggagctt
     6901 cccaggccaa gaagcggacg gaggctttac gcattacaga gccaattcag attgacggga
     6961 agcttgatga ggcaccatgg cagaaagcct cacccgccgg tgatttcatt atttacaacc
     7021 cgcataacgg catgccctca aaattcagga ctgaggtcag gcttctttat gatgatgatg
     7081 cattgtatat cggagccata atgtatgaca gttatcctga cagcatcttc acccagcttg
     7141 gtcagaggga tgccggtgac atcaacgcag accatttctg gattgagatt tcgccattca
     7201 atgacgggct caatggtgag atgtttaaag tatctgcttc caacgtccag atagacaata
     7261 agatgtctac cgctgacagt tggatgcaca gagattcgtg ggatgctgta tggcacagta
     7321 ataccagtat aagtgacgag gggtggattg cagaaatgaa gataccctac tctgccctgc
     7381 gctttccaag gtctgcatca cagctctggg gcattaattt ctggcgtgaa gtgcgccgca
     7441 acagggagac ctcatcatgg aatttcgtca cgaaggaatt cgggtcgccc atttcgcaca
     7501 tgggtgagct taccgggata cgtgatgtta aaccaccgct gaggctttca cttgtgccat
     7561 atgtctcagg ttaccttgaa catgtaactg atgaatcagg agtaggcacc tcatataacg
     7621 ggggactcga cctcaaaatc ggcctgaccg agagcttcac ccttgatgca acgctgatac
     7681 ccgatttcgg tcaggtgcag tctgatgacc atgtattgaa cctgtcgccc tacgaggtca
     7741 ggtacaatga gaagagacct ttcttcatgg agggtaccga gctgttcaac aagggaggca
     7801 tattctattc ccgccgcata ggcggaacgc ctcatctgat acgggatgtc tatggcatgg
     7861 ttggagagaa tgagacagtg atccggaatc cggcagaggt tagtctgctt aatgcaacca
     7921 aaatatcagg aaggacaagc agcggcctcg gcatcggtct cttcaacgcg gtgaccaaca
     7981 ccacctgggc cgtggtggag aacaagctta ccggggaaac caggcatatc atgactgagc
     8041 cgctgacaaa ctacaatatg cttgtgcttg atcagaccct gaggaatgac tcatacgtga
     8101 gtgtgatgaa caccaatgtg atcagggccg gcaaaaggaa tgaaaacttc tatactgcca
     8161 atgtctccgg ctttgaggct cttgtcaaga cagccgacag gctgtggtca gtttccgccg
     8221 gcgctgtact gagccagaag tactactcat ccgatcccac cgctcttggt cattcactta
     8281 acttcaatgc cggcaggaca ggagggaaat tccgcgctga ctacagattt tcaatgatga
     8341 gtgacaccta tgatccgaat gacatgggtt atctccggaa gaataacgaa atagaacact
     8401 cggttgattt ctcatacaat acctatgagc cctttggcaa tatcatgact acccgtacct
     8461 cactcgattt cagttatggc cagctataca atccaagggc attcacaacc gccgaagtca
     8521 ggcttgacgc catgattctg ctgatgaatt tctggtctct ctcacttgac cttgagctca
     8581 caccctgggg tgaggatgat tattttgagc cgcgtacccc tgacatgtca atgttttatc
     8641 accggcctgc tgcatttgaa gccagtttcc ggggtgatac cgacagaagc aagaggtttt
     8701 acattcaggg aaatgctgag tatatgaaag cctggtcaga ctacggccag tacggatatg
     8761 aatggtcact ccagcctgaa ttcaaggttg gcaggcgctt ctccttcagt tattccatat
     8821 cggttgaaga cctgatcaat gatatcgggt ttgtaccggg caatccacac gagggaatca
     8881 ctttcggaaa acgtgatgtc acaaccataa ccaataccat ttcgggagca ttcatcttct
     8941 cagccaattc ataccttaca ctgaggggga ggcactactg gtcaagagct gattatgacg
     9001 gggattattt tacccttcag cctgatggca gccttttaga ccgtatctat tccaccccac
     9061 tgaacaatag tgacgtaaac accaatttcc tgaatattga tgtggtatat acctggcggt
     9121 ttgctccggg aagtgagctt tcactggtat ggaagaactc catctggtca gaaggtgatg
     9181 tcattatccg gaacggtttc gacaatttcg gtgatcttct atccatgccg cagacaaaca
     9241 gtctgtcatt gaaaatttta tactaccttg actaccagaa tttcagaaag attttcaagt
     9301 caaagtaatt aattttcaga atgatacaaa gaatacagac attgttcctg ttggctgtgg
     9361 tattcctgtc gtcgcttatg ctcaccggcg accttatcca gctgacgaca gatggtggcg
     9421 ccctgttcag catcagtttc cctggtatga aaaaccaggc aggagaggtg gtgcaacggc
     9481 tttggccgat gagtgttatc cttgcattgg ttccgctcct tgcactcatt gccctgttcc
     9541 tctacaagaa gaggcctttg cagatgaggt taaccatgct ggtcctcctg ctttcgctcg
     9601 gaaccctgat actgggagct ttttatgtgc tgatgtttga cagaaagata gatgtgaata
     9661 tcatatggaa ggtcaaggtt ctctttccgt tcatatctgc catcctggcg tggctcgcct
     9721 atcgcgccat cctgaaggat gagctcaggg tcaggtctta tgaccgtctc agatgatctg
     9781 cagtctctcc tgacagatct gccactgacc ggatgaatcc ccgcagacag cttcagataa
     9841 tttccagtct ctcctggtcg gtgattttct cgcagtagag gcacttcagc gccaccggtt
     9901 tctttgacac caccctgaag tgagtggtca ccttctcaag gtttgtaatg catttgggat
     9961 tcatgcattt ggctattgca ttgatggtgt caggaacctc cacaacgcgc ttctctatca
    10021 cctgatagtc acgaataata ttcagcttcg ctttgggagc cacaagggcg atcctgtcta
    10081 tatcatcatc ctcgaaaaac ttccctgcaa tttttatgat agccttttta ccgctggttt
    10141 tgctttcgag gttggtgcca aatgttatct ggttcctgat acggtcaaga ccgagaatct
    10201 ttattacctt gaaaagagag ctggcaggaa tgtgatcaat cactgtaccg ctttcaatgg
    10261 cactgaccag caactgcttc ttatcgttta tgttcttcat ggcttcttat tttaatccaa
    10321 gtaataagca tattattgcc tgcctcgtgt aaaccccgtt cagtgcctgt tcgaaatagt
    10381 atgccttcgt gttgccatca acatcagtgc taatctcatt cacacgtggc agaggatgca
    10441 gaatcctcat ttccggtttg gtatctgaca acatgtaatc atgcaataca taggcattct
    10501 ttaccttttc atactccatc gggtcagaga atcgttcacg ctggacacgg gtcatataga
    10561 cgatgtcagc catgttgata acctcgttca ggtcagtagt ttcatgatag tcaaggccaa
    10621 ggttcctcag gtagagcttt tgttcatcag gcatcttcag ctcttccggc gcaacaaaat
    10681 ggaaagatgt tttccaacgt gacatggcca tcagaagtga gtgaaccgtc cggccgtact
    10741 tcaggtcacc caccatgcag atattgagat tgtcaagtgt accctgcgtc ttctgtatgg
    10801 agtagagatc gagcagtgtc tgggtgggat gctgattggc cccgtcgcct gcattgatga
    10861 cgggcacagt cgctatctca ctagcatagc gtgcgcttcc ttcaatgggg tgtcgcatca
    10921 caataaggtc aacgtagttg ttgacggtcc tgatggtatc attcagactc tctcccttgg
    10981 caacgcttga attggaagag tcagtgaatc ctatcacttt cccccccagt ttgttgacgg
    11041 cgctctcaaa actcaggcgt gtgcgggtgg agggctcgaa gaagagcgtt gccacaacct
    11101 tgcctcgaag tatatcctga accggattct tttcaaattc tgcggccaga gtgagaatct
    11161 ttagtatctc ttcagtggtc agatcatcaa ttgagactaa gcttctggat ttcattattt
    11221 tatgttttat atgcctgaac tctggcaaag ttattataac tgcataagct attttctgcc
    11281 tgtttaaaac tagttaataa aaaacagccc gcctattctg ttgcgagcca tttcatctgt
    11341 agtccgctga tcttgttcag cctgcctgta acatggtcag ccatggaagc cctgaacctg
    11401 atctcaactg tcacgtcagt gccgctgtaa cggtgttccg actgttcaca tgattcatcc
    11461 ctgaggactc tcatgacatc attcatcgat gtatagggaa aggagacaga ccatcgtacc
    11521 atgatatatt tctcaaccat cgttgctttg tcaagggcat cggcggctgc attgcggtac
    11581 gcgtttatca gtccgcccac acccagcagg gttcccccga aatacctgat taccacaatg
    11641 agaacattgg tcagttcgcg tgcattgatc tgtgcaagga ttggtcttcc tgcggttccc
    11701 gatggctcac cgtcatcact gacacgccat gcctgcctgt cgggtcccag tacccatgca
    11761 aagcagtgat gcctcgcatc atggtactcc tttctgagct ccgcgagcct ggcctttatc
    11821 tcctccctgg aggtcacggg aagagccaaa gcgataaacc tgcttccacg gtctttgaac
    11881 agtccctgtg actctccctc tatcgtttta tatgtgtct
//
LOCUS       DAOPBK010000075        11784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5365_length_11784_cov_5.858897, whole genome shotgun sequence.
ACCESSION   DAOPBK010000075 DAOPBK010000000
VERSION     DAOPBK010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5365_length_11784_cov_5.858897"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2247
                     /locus_tag="PLA17_05875"
     CDS             <1..2247
                     /locus_tag="PLA17_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK84977.1"
                     /translation="WLFIRQDSANVDKILPGIIPNHKWEEVTLPHSAVIEPLVVTASQ
                     WTGICWYKKVYKPDRRQRHRHTGLLFDGAMHDAIVYLNGAEIARHTGGYLPFYVNLSN
                     DLEYGRENEIMVKLDNRENPHIPPGKPLGELDFLWYSGLYRNVSLYVTDKLHITAPPE
                     YQSTLGGGIITGVRDVSRESATLTLSVHVSNDDHIPRSFRMKNTLLDAAGLEVASSLS
                     ELIDMEPGQSQRLETRMIVMQPSLWTPATPFLYTISTELLSESSTVIDHRETRTGIRT
                     VRITPDKGLLLNSEPVRIMGTNRHQEYPYIGYALSDNASYRDAFKIREAGFNLVRCSH
                     YPPAPAFLDACDELGLMVIDAIPGWQFMGDSLFAEASVRDTRMMCRRDRNHPSVVMWE
                     SSLNETLMPVSFLARLHQTVKDELPFGNNLTCSWMDTICDVFIPARQHAPAPRYWKDY
                     RKAKPLFICEYGDWEYYAQDAGLNQTEFSDLLPAERNSRQLRGKGDRHMLQQAWNFQE
                     AHNDNLNGPGFGDANWVMFDYNRGYAPDIETSGIMDIFRLPKFAWYFYRSQTIEGPVC
                     FIASHNLPSSGNIVRVYSNADTVKLFRNDTLIAVQGPDTDINAVNLLHPPFTFTLPDY
                     SPGTLKATTLRNGKESASHSVTTPGRPAAIRLSADFSNKPLRADNADVIFVYATVTDS
                     AGNQVYTSDTMVEFALRGNASLIGDNPARAEGGIATILLRAEDGGKIMIKASSPGMSE
                     AELMTVSK"
     gene            complement(2277..5573)
                     /locus_tag="PLA17_05880"
     CDS             complement(2277..5573)
                     /locus_tag="PLA17_05880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPK84978.1"
                     /translation="MRPGRILLFLLAVVLTSFLLSLLTGSWRPGKEVIHHEQESTAHD
                     SVQTADTGSLGGSFHSGSDAGSRSLSSAGSSIDSCATSPRSSAIDSRISAPGISATDS
                     RTGSSSNSDAGSRSISSAGSATDSRTNSSGSYDTGSRAGLYVNNLARAPFHPGNAIKD
                     SVASGRQVRVIFYGDSQIEGDRVTSFLRRELREEGGGTGPGMISPVMPVMYTRSWVVR
                     SSSNWKRYTLLDYRNGTLSHNRLGPMLALCRFTPPGDSMQTRSFATVRISAVSGADPA
                     VSRYDNLRIFYGNNYDTVLVGIKSGSSLVDFAMLQMGEGPMEYSVPLPSLSEVTVEFT
                     GRNSPDIYALSLESSTGVIVDNVPVRGSAGLEFVMTDSRGFEGCYSDLKPDIIFLQFG
                     LNVVRNVRSEYHYYEEGLVKQVDYLKKVSGGVPVVLVSVTDMALRDNDTIRQFPNIRA
                     IRDAQKAVADRTGAVFWDAWESMGGPGSILTWYNHNPPLSSRDLTHLSNAGTDTIAAR
                     IYSSLLASRPVTAPAMAQVYQSAADSAPAGLRTTADLLLARMTAQPDTALFATERDTG
                     SVITAASGEESEETAISGRKEHSITSPVIGRIASLLRYHPGQSFIFTTLSFWIFFLVV
                     MTGFALLHRRRAMCHTWLLVVSLYFYYRTGGLFIILLLLTTLLTFFTAIMTGRAGTRA
                     GKRFWLVTNLVILLGFLSYFKYAGFFTDLINSLFNTNLVSRDIFSAWSNSLFGTKFDV
                     STIMLPVGISFYTFQALSYSIDVYRGQMAAERNIVDFAFYLTFFPQLVSGPIVRASEF
                     IPQMHGNYTISRNEFGYGLFLILQGLIKKMLISDLISTGFIDRVFESPAIYSGFENLM
                     AVYGYGLQIYVDFSGYTDIAIGIAMLMGFRLPLNFNSPYKAASPGEFWRRWHISLSRW
                     LKDYLYIPLGGSRKGPLRTGINLMVTMLLGGLWHGAATRFVVWGGLHGVALVVEKIWR
                     WLFGKVSVRKRGAHIAGILITFNFVSFMWIFFRACTMEQAVIMINQIFSSFSPGDYGL
                     VLISYLPVVVLIVSGYILHFLPVTVKESYRGLFIRLPLAAQLLVALAVALLLYKVGSE
                     VVQPFIYFRF"
     gene            complement(5587..7149)
                     /locus_tag="PLA17_05885"
     CDS             complement(5587..7149)
                     /locus_tag="PLA17_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPK84979.1"
                     /translation="MKKNLKKIILPLFLSIVALPALAVNDTIIIRSDESTELIEHDLD
                     SLLNNWFIRMSVAGADLSSADSVIHEFSDSIYKDRLSRINSIIIPPYNNIVRNHIHVY
                     TERKVDKFRVMLGLQDYYFPMIEDIFDSYGLPVELKYMAVIESALNPNAVSRAGATGL
                     WQFMYSTGRMYGLTINSVVDERRDPVRATHAAAKYLKDLYGIYNDWILVIAAYNCGPG
                     NVNKAIRRSGNRKDYWEIYYRLPRETRGYIPAYVAATYAMNYYREHNITPIQINLPLA
                     VDTVMVSTDMHLEQITAVLDLPLEELRAMNPQYRTGLVPGRSRPSPVTLPLDRLGDFI
                     AMTDTITGYRKDQYLTTVNQTSMPQSTASTFTPPDVTGKTRLTYTVKEGDNLGFISEW
                     YDVPLSELRYWNNIYRNIIRVGQKLTVYVDPSKAERYSKINSMTFAEKQQLEGKPVGN
                     TSLALASPQPLPVTSGEGDYVLYTVRYGDTVWDIAKKFSGVSVSDILSLNNITDASRI
                     KVGQKLRIRKKS"
     gene            complement(7157..7702)
                     /locus_tag="PLA17_05890"
     CDS             complement(7157..7702)
                     /locus_tag="PLA17_05890"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPK84980.1"
                     /translation="MRKERAKRYSIEPIRATMYAAALPGLGQIYNRKYWKVPIVYAGF
                     GALGYAVTFNTKNYNTFINAYQDFTDIIPETDSYLDLLVNMNPEQYDPVLHPKTYNPS
                     TESWVKTTLLNGVDYYRKYRDLSYIGIAAWYLLTIIDAHVDASLFDYDISDDIRASVV
                     PVSFNYSGVSPGITFALLKTF"
     gene            complement(7825..8712)
                     /locus_tag="PLA17_05895"
     CDS             complement(7825..8712)
                     /locus_tag="PLA17_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPK84981.1"
                     /translation="MNTSKKSPLGRGLGALIEGVEKEALEKKVEANLQIEIDAIDGNP
                     FQPRTRFDSQSLEELAASIRQMGIVVPLTVRETGDGRYQLIAGERRLRAARMAGLTHV
                     PAYVRTADDTAMLEMALVENIQREDLDAIEVAITYQRLIEECSLTQEQLSDRVGKQRS
                     TVANYLRLLRLPAEIQLGIRNRNLTMGHARTLVNIEDPARQINVFYHIIEEDLSVRAT
                     EDLVRRLQREAAKDPAKIEKKNKLNAEYQELSKQLSNLFQSDVQFRINQKGRGKIVIP
                     FSDSEEMERIIGLLDKLNR"
     gene            complement(8730..9509)
                     /locus_tag="PLA17_05900"
     CDS             complement(8730..9509)
                     /locus_tag="PLA17_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK84982.1"
                     /translation="MSRIIALANQKGGVGKTTTAINLAASLAALEKKVLIVDADPQAN
                     ATSGVGIDLKQVKTTIYDCLIDGVNPRQVILQCGIDNLSIIPSNIDLVGAEIEMLERP
                     EREKTLKKILAPIVTDYDYVLIDCSPSLGLLTVNALTAADSVIIPVQCEYFALEGLGK
                     LLNTIKIIQSNLNQSLEIEGFLLTMYDSRLRLANQVVEEVTRHFRQMVFNTIIQRNVK
                     LSEAPSYGQPAILYDAESRGTVNYLNLARELLERVEARQNN"
     gene            complement(9735..10859)
                     /locus_tag="PLA17_05905"
     CDS             complement(9735..10859)
                     /locus_tag="PLA17_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPK84983.1"
                     /translation="MKRIVGYISLLSLVLVSVISCSGSAGKRGTKAVEDAAVATESDP
                     RPAKRLIEVLSPADNASYVCTDRIVFSVGPAAGQTEVDSVQVWVGSRLARTLTTIPAM
                     AELDMDGNPGRISVRAVAYSQASRPQTVTLFINLVSDITPVTYRYRVVKSYPHDRRAY
                     TQGLVYDNGFFYEGTGQQGESSLRKVEAETGKVISQVNLDGSLFGEGVSLLGDFIYQL
                     TWTSKVGFVYEKETLRQVKRIYYQTQGWGLATMGDSLVMSDGTNVIWFLDADFNVLSS
                     VDVWDNKGVVDNLNELEIIDGELWANIWQTDRIARIDPHTGKVLGYVELNNLLPREER
                     DSMTDVLNGIAWDAEGRRLFVTGKYWSRLFEITVSPVSRN"
     gene            complement(10869..11273)
                     /locus_tag="PLA17_05910"
     CDS             complement(10869..11273)
                     /locus_tag="PLA17_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84984.1"
                     /translation="MKIAVDFDGTIVEHEYPAIGKEKLFAFITLKELQKRGALLILWT
                     FREGKELQEAVDFCSQNGIEFYAVNRNYPEEQWTDGNPRKINVDIFIDDRNAGGFPGW
                     GEVLHMIDPWTEREEEAMRRIKRRRFSLFGKK"
     gene            11364..>11784
                     /locus_tag="PLA17_05915"
     CDS             11364..>11784
                     /locus_tag="PLA17_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPK84985.1"
                     /translation="MIKHIPNTLTLLNLICGFAAIVCVIKGYNDYASYCIIVGMVFDF
                     SDGFAARVLKAYSALGKELDSLADMVTFGVAPGSIVFNLLTAGGMTELKAFAVAVIIP
                     AASALRLARFNNDPEQSSSFRGLATPASAFTIVSVSLA"
BASE COUNT         2846 a         3116 c         2970 g         2852 t
ORIGIN      
        1 tggctcttta tcaggcagga ctcagccaat gtagacaaga tactgccggg aataattccc
       61 aaccacaaat gggaagaggt gacactgcca cattcggcag taattgaacc tcttgttgtt
      121 acagcaagtc agtggaccgg tatatgctgg tataaaaagg tgtacaagcc tgacaggagg
      181 caaaggcaca ggcacaccgg actccttttt gacggagcca tgcatgatgc tatcgtttac
      241 ctgaatggtg cagagatagc acgtcatacc ggaggatatc ttcccttcta tgtcaatctg
      301 agcaatgatc ttgagtacgg cagggaaaat gagattatgg taaagcttga caacagggaa
      361 aatccgcata tccctcccgg caagcccctt ggcgaactcg atttcctgtg gtacagtggc
      421 ctttaccgca acgttagcct gtatgttact gacaagctgc acattaccgc accgcctgag
      481 tatcaatcca cactgggcgg agggattatc accggcgtca gggatgtgag ccgcgagtcg
      541 gccaccctga ctctctcagt gcatgtaagc aatgatgacc acattcccag gtcattcagg
      601 atgaaaaata cccttcttga tgcggccgga ctggaggttg cctcttctct gtcagagctt
      661 atcgacatgg aaccgggcca gagccagcgt ctcgagacca ggatgattgt catgcagcct
      721 tcattatgga ccccggccac acctttcctc tataccatat ctaccgaact gctctcagag
      781 agcagcacag tgattgacca tcgggaaacc agaaccggta taaggacagt gaggatcacg
      841 cccgacaagg gactgctcct gaacagtgag cctgtcagga ttatgggaac taaccggcac
      901 caggaatatc catacatcgg atatgccctg tcagacaatg ccagttatcg cgatgccttc
      961 aagatcaggg aggcaggctt caaccttgtc agatgctccc attatcctcc ggcgcctgca
     1021 tttcttgatg cctgtgatga gttgggcctg atggtcattg atgccatacc cgggtggcag
     1081 ttcatgggcg actcgctttt tgcagaggca tctgtgcgtg atacacgcat gatgtgccgc
     1141 cgtgaccgca accacccgtc agtggtgatg tgggagtcat cactgaatga gaccctcatg
     1201 cctgtcagtt tccttgccag actccaccag acggtaaagg acgagctccc atttggcaat
     1261 aacctgacct gcagctggat ggatactatc tgcgatgttt tcatcccggc acgccagcat
     1321 gccccagctc cccggtactg gaaagattac cgcaaagcaa aacctctttt catatgtgag
     1381 tacggcgact gggagtatta tgcgcaggat gcaggattaa accagacgga gttttctgac
     1441 ctcctgcctg ccgaaagaaa ctcaagacag ctgagaggca aaggcgacag gcacatgctg
     1501 cagcaggcat ggaacttcca ggaggcgcat aatgataacc tgaatggtcc gggattcggc
     1561 gatgccaact gggttatgtt tgactacaac aggggctatg ctcccgatat tgaaacctca
     1621 ggcatcatgg acatattcag gctgcccaag ttcgcctggt atttttaccg gagccagacc
     1681 atagagggcc cggtatgctt tatcgccagt cataacctgc cctcctcggg caatattgta
     1741 agggtataca gcaatgccga cactgtgaag ctcttccgca atgacacgct tattgcggtt
     1801 cagggacccg acactgacat caatgctgtc aatcttctcc atccaccatt caccttcaca
     1861 cttccggatt attcaccagg aacattgaag gccaccaccc tgcgcaacgg aaaggagagt
     1921 gcgtcacatt cagtcaccac cccgggcagg cctgcagcca tccggctgtc agccgacttc
     1981 agcaacaagc cgttacgagc cgacaatgct gatgtcatct ttgtttatgc tacagtaact
     2041 gattcagcgg gcaaccaggt ctacacctct gataccatgg tggaattcgc cttgagggga
     2101 aatgcctccc tgattggtga caatccggca agagccgaag gcggaattgc caccatcctg
     2161 ctcagggccg aagatggcgg caaaataatg atcaaggctt cttctccggg aatgtcagaa
     2221 gcagaattga tgactgtgtc gaaatgactc cgcttcccgg gttgccggga gaataatcag
     2281 aaacggaaat agatgaaggg ttggaccacc tctgacccca ccttgtagag gagcagtgcc
     2341 acagccaggg caaccagcag ctgtgccgcc agcgggagtc ttataaagag tcctctgtat
     2401 gactccttga ctgttacggg caggaagtga aggatgtatc ctgaaacaat cagtaccacc
     2461 acaggcaggt atgatatcag caccagacca tagtctcccg gagagaacga tgagaatatc
     2521 tggttaatca taatcacagc ctgctccatg gtacaggccc tgaagaatat ccacatgaag
     2581 ctgacaaagt tgaatgtgat aagtattccg gcgatatgtg cgccacgttt tcttactgac
     2641 accttcccga acagccagcg ccagatcttt tccacaacca gtgccacccc gtgaagcccg
     2701 ccccagacca caaaacgtgt ggcagctccg tgccacagac caccgagcaa catcgtgacc
     2761 atcaggttga tgccggtgcg caacgggcct ttcctgcttc ccccgagagg gatatagaga
     2821 taatccttca gccacctcga aagggatata tgccacctcc tccagaattc gccaggactg
     2881 gcagccttgt acggcgagtt aaagttcagc ggcagtctga atcccatcag catcgcgata
     2941 ccgatggcga tatcggtata gcccgagaag tcaacgtaga tctgcaaacc gtaaccgtac
     3001 accgccatca ggttttcaaa acccgaatag attgccgggg attcaaaaac cctgtctatg
     3061 aacccggtgg agatgagatc agagatcagc atcttcttga tcaatccctg gaggatgaga
     3121 aacaatccgt acccaaactc gttgcggctg atggtataat taccgtgcat ctgcgggatg
     3181 aactccgacg cccttacgat gggacctgac accagttgcg ggaaaaatgt caggtagaat
     3241 gcaaaatcta caatgttccg ttctgccgcc atctgccccc ggtaaacatc aatactgtag
     3301 ctgagtgcct ggaaggtgta aaatgagatg cccactggca gcatgatggt gctgacatca
     3361 aatttcgtcc cgaacagact gtttgaccag gctgagaaga tgtcacggct gaccaggttg
     3421 gtgttgaaca gagagttgat gaggtctgta aagaacccgg catatttgaa gtatgagagg
     3481 aaaccaagaa gaatcacaag gttggttacc agccagaatc ttttaccggc tctggtgcct
     3541 gcgcggccgg tcatgatggc agtgaagaag gtaagcaggg tggtgagcag cagaagtatg
     3601 atgaatagtc cgcctgtacg gtaataaaaa tagaggctga caaccagcag ccatgtgtgg
     3661 cacatggctc ttcgcctgtg cagcagggcg aaccccgtca tcactacgag gaagaaaatc
     3721 cagaaggaga gtgtggtaaa gatgaatgac tgcccggggt ggtacctgag cagagacgca
     3781 atcctgccaa ttaccgggct ggtgatgctg tgttcttttc tgcctgagat agctgtttct
     3841 tcactctcct ccccggatgc ggctgtgata actgaaccgg tatcccgctc tgtagcaaag
     3901 agcgcggtgt caggctgagc agtcatgcgg gccagcagta aatcagccgt ggttctcaga
     3961 ccggcgggag cggaatctgc ggctgactga tacacctgtg ccatagctgg cgccgtaaca
     4021 ggccgtgatg ccaggaggga ggaataaatc ctggccgcga tggtgtctgt tcctgcattt
     4081 gaaaggtgcg tcagatcccg ggatgaaagg ggaggattgt ggttgtacca ggtgagtatt
     4141 gatcccgggc cgcccattga ctcccatgcg tcccagaaca ctgcaccagt acggtcagca
     4201 accgccttct gggcatcacg gatggccctg atgttgggga attggcgaat ggtgtcatta
     4261 tccctcaggg ccatgtcggt cacgctgacc agcaccacag gaacaccgcc ggagaccttc
     4321 ttaaggtaat cgacctgctt taccagtccc tcttcatagt aatggtactc gcttctgaca
     4381 ttccttacca cgttcaggcc aaactgcagg aagatgatgt caggcttcag gtccgaatag
     4441 catccttcga aaccacggct gtcagtcatt acaaactcca gtccggcgct accgcgaacg
     4501 ggcacgttat ctacaatcac gcccgtgctg ctctcgaggc tcagtgcgta tatgtcaggc
     4561 gaattgcgtc cggtgaactc taccgtcacc tcagagagtg acggaagggg aactgagtac
     4621 tccatcggtc cctcgcccat ctgcagcatg gcaaaatcaa ccagtgagct gcctgacttt
     4681 atgcccacga gaaccgtgtc ataattgttg ccgtagaata tcctgaggtt gtcataccga
     4741 gatacagccg ggtcagcccc ggagacggcg cttattctca cggtggcaaa ggagcgtgtc
     4801 tgcatgctgt cacccggagg ggtgaaacgg cacagtgcca gcatggggcc cagcctgttg
     4861 tgggacaatg tcccgttcct gtagtcaagc agagtgtatc tcttccagtt tgatgaggat
     4921 cgtacaaccc atgaccttgt gtacatcacc ggcatcaccg gggagatcat gcccgggccg
     4981 gtgcctcctc cctcctccct cagctcacgc ctgagaaaag aggtgacacg gtcaccctca
     5041 atctgtgagt caccgtaaaa gattaccctt acctgcctgc ctgaggcaac tgaatccttg
     5101 attgcattgc ccggatgaaa gggtgctctt gccaggttat tgacatacag accggcgcgt
     5161 gacccggtgt catagctgcc ggaagagttg gtgcgggaat cagttgcgga accggccgaa
     5221 gaaatactgc gtgacccggc atcagagttg ctggaagagc cggtgcggga atcagttgcg
     5281 gaaatgccgg gagcactgat gcgcgaatca attgcagagc tgcggggaga agtggcgcat
     5341 gaatcaattg aggaaccggc cgaagaaaga ctgcgtgacc cggcatcaga accggagtgg
     5401 aaagaaccac caagtgaccc ggtgtctgct gtttgtacgc tgtcatgtgc tgtgctctcc
     5461 tgctcgtgat gtatgacctc ctttcccggt cgccaactgc cggtcagcag actcaggagg
     5521 aacgatgtca gaacgacagc aagcagaaat agcaggattc tgccggggcg catttttctt
     5581 tattattcag cttttctttc tgatccgcag cttctgaccc acctttatcc ttgaagcatc
     5641 ggtgatatta ttgaggctga ggatgtcact tactgaaaca ccactgaact ttttggctat
     5701 atcccaaacc gtatcgccat accttacggt atataacacg tagtcacctt ctccggaggt
     5761 tacaggtaat ggctgcggtg atgccagtgc caatgatgtg tttcccacag gcttgccctc
     5821 caactgctgc ttctcggcaa aggtcatgct gttgatcttg ctgtaacgct ctgcctttga
     5881 aggatcgacg taaaccgtaa gcttctgccc caccctgatg atgttgcgat atatgttgtt
     5941 ccagtatctc agttctgaaa ggggtacatc ataccattcc gagatgaatc cgagattatc
     6001 tccctccttc acggtgtatg tcagcctggt cttgccggtg acatcaggcg gtgtaaacgt
     6061 cgatgcggta ctctgtggca ttgaggtctg gttgaccgtg gtcaggtact ggtctttcct
     6121 gtaccctgta atggtgtcag tcattgcgat gaagtcaccc agcctgtcaa gtggcagagt
     6181 gacggggctg ggcctgcttc gtccgggaac aaggcctgta cgatattgcg gattcatcgc
     6241 cctgagctcc tccagcggca ggtcaagcac cgctgttatc tgttcaagat gcatgtcggt
     6301 tgacaccatt acagtgtcaa cggcaagcgg cagattgatc tgaatgggtg tgatattatg
     6361 ttcacggtag tagttcatcg cataggtggc tgcaacatat gccggtatat aaccgcgggt
     6421 ctcacgggga agacggtagt atatctccca gtaatccttc ctgtttcccg accggcggat
     6481 agccttgttg acattgcccg gtccgcagtt gtatgccgca attaccagga tccagtcatt
     6541 gtagataccg tacaaatcct tcaggtattt tgctgcagca tgggttgccc tgaccggatc
     6601 acgcctctca tccaccaccg aattgatcgt cagaccgtac atcctcccgg tgctgtacat
     6661 gaactgccac aaccccgttg cacctgcccg gctgaccgca ttgggattaa gggcagattc
     6721 aatgactgcc atgtacttaa gctccaccgg cagcccgtaa gaatcgaaga tatcctctat
     6781 catagggaag taatagtcct gcagacccag cataaccctg aacttgtcca ccttccgctc
     6841 cgtgtataca tggatgtgat tgcggacaat attgttgtag ggtggaatta taatggagtt
     6901 gatacggctg aggcggtcct tgtaaatcga atcgctgaat tcatgtataa ccgaatcggc
     6961 agaggaaaga tcggcacctg ctaccgacat cctgatgaac cagttgttca ggagactgtc
     7021 gaggtcatgc tctatcagct ctgttgattc atcactcctg attattattg tatcattaac
     7081 cgccagggcg ggaagcgcta ctatcgacag gaacagcgga aggatgattt tcttcagatt
     7141 cttcttcata ataaaattaa aacgtcttta acagggcaaa tgttataccc ggagatactc
     7201 cggaatagtt aaaactcacc ggcacaactg aagccctgat gtcatcggat atatcatagt
     7261 caaaaaggct ggcatctaca tgcgcgtcaa taatcgttaa aaggtaccat gccgcaatgc
     7321 caatatacga aagatcacgg tacttccggt aatagtcaac accgttcagg agagtggttt
     7381 ttacccacga ctcggtggat ggattgtagg ttttagggtg gagtaccggg tcatactgtt
     7441 caggattcat gtttaccaga aggtcgaggt aactgtcagt ctccggaatg atatcggtga
     7501 aatcctggta tgcatttata aatgtgttgt agtttttggt attgaaggtg acggcatagc
     7561 caagtgcacc gaaacctgca tagactatcg gcaccttcca gtatttgcgg ttgtatatct
     7621 gaccgaggcc cggcagtgct gcagcataca ttgtcgccct gattggctca attgaatatc
     7681 tctttgccct ctcttttctc acaggcacag tgtcagccgg ctgggccgtg ctggttaagg
     7741 taatgacaaa aaggagaaaa gctgctatag tgctgctgag cttcaaggac aaacatttta
     7801 gtttgccggc aaatataaat aaaattacct gttaagcttg tcgagcaatc ctattatgcg
     7861 ttccatctcc tcgctgtcag agaatggaat tacaattttt ccccggcctt tttgatttat
     7921 ccggaattga acatctgact ggaagaggtt gctgagctgt tttgacagct cctggtattc
     7981 ggcgttgagc ttattctttt tttcaatttt tgcagggtct tttgccgcct ccctctgaag
     8041 gcgcctgacc aggtcttccg ttgcccgtac agagaggtcc tcctcgatga tatgatagaa
     8101 aacattaatc tgcctggccg ggtcttcgat attcaccagg gttcgtgcgt gtcccatggt
     8161 gaggttacgg ttgcggatgc cgagctgtat ctctgcgggc agcctcagca ggcgcaggta
     8221 gttggccacc gttgaacgtt gtttccccac cctgtcactg agctgttcct gggtcaggct
     8281 gcattcctct attaggcgct ggtaggttat cgcaacctca atggcgtcga ggtcttccct
     8341 ctggatattc tccacgaggg ccatctcaag catagcagtg tcatcagccg tgcggacata
     8401 ggcaggaacg tgtgtcagcc cggccatgcg cgctgcacga agacggcgtt caccggcaat
     8461 gagctggtaa cggccgtcac ctgtctccct gacggtgagc ggaacaacaa tgcccatctg
     8521 gcggatggag gcggcaagct cctcaagtga ctggctgtcg aaacgggtac gtggctgaaa
     8581 aggattgcca tcaattgcgt cgatttctat ctggaggttg gcctccacct tcttttcgag
     8641 agcctctttt tcaacacctt caataagggc tcccagacct ctgcccagcg ggctcttttt
     8701 gctcgtgttc attatgcgga ttaacgtttt tagttattct gcctggcttc caccctctca
     8761 agcagttcac gggccaggtt gagataattg accgtacccc ttgattcagc atcataaagt
     8821 atggcaggct gcccgtagct gggagcctcc gagagcttta cgttgcgttg tatgattgta
     8881 ttgaagacca tctgccggaa atggcgcgtg acctcttcaa ccacctggtt agccaggcgc
     8941 aggcgcgagt catacatggt cagcaggaac ccctctatct caagtgactg attcaggttg
     9001 gactgaatga tcttgatggt gttgagcagc ttgcccagcc cctcaagcgc aaaatactca
     9061 cactgcacgg gaatgattac cgagtcggca gccgtgagtg cattgaccgt cagcaggccg
     9121 agcgacgggg aacagtctat cagcacgtaa tcataatcgg taactatggg tgcaagtatc
     9181 ttcttcagcg ttttctccct ctcgggtctc tcaagcatct ctatctccgc ccccacaagg
     9241 tcaatattgg aaggtatgat cgacaggttg tcaatgccgc actgcagtat aacctgccgt
     9301 ggattgaccc cgtcaatcag gcagtcatat atggttgtct tcacctgctt caggtctatg
     9361 cccacccccg aggtggcatt agcctgcgga tcagcatcaa caatgagaac cttcttttca
     9421 agcgcggcca gactcgcagc gagattgatt gcagtggtcg tcttccctac tcctcctttc
     9481 tgattggcca gtgctattat ccttgacata taatcctgtt tttcttttta ttaatgagct
     9541 tcaaatttac aattaatgcc attctccgga aaccggaccc gtatgttatt gtaaacaatt
     9601 ttttcggggt tattaacacg ttttcaacag gcggtccggt ttgaccggtc aattcagttg
     9661 cgcgagacgg gtgagacagt aatctcgaaa agacgggacc agtatttgcc ggtgacaaag
     9721 agaggctggc caattcagtt gcgcgaaacg ggcgagacag taatctcgaa aagacgggac
     9781 cagtatttgc cggtgacaaa gagacgccgg ccctcagcat cccaggcaat gccgttaagc
     9841 acatccgtca tggaatcgcg ctcctcacgc ggaagaagat tattcagctc aacatatccc
     9901 agcaccttcc cggtatgagg atcaatccgt gcaatcctgt cagtctgcca aatgttagcc
     9961 cacagctcgc cgtcaatgat ctcaagctca ttcagattat cgacaacacc cttgttgtcc
    10021 cacacatcaa ccgaggaaag aacattgaaa tcagcatcaa ggaaccagat cacgtttgtt
    10081 ccgtcactca tcaccaggct gtcacccatt gttgcaagtc cccatccctg ggtctgataa
    10141 tagatacgct ttacctggcg gagagtctcc ttctcatata caaaccccac cttgctggtc
    10201 catgtgagct ggtagatgaa atctcccagc aacgacaccc cctcaccgaa cagggagccg
    10261 tcgaggttca cctggctgat taccttgcct gtctccgctt ccacctttct gagtgacgac
    10321 tctccctgct ggcctgttcc ctcataaaag aacccgttgt catataccag tccctgtgta
    10381 taggctctcc tgtcatgcgg gtagcttttg acaaccctgt aacggtacgt aaccggtgtg
    10441 atgtctgaga ccaaattaat gaagagggtc accgtctggg gcctggaggc ctgtgagtat
    10501 gccactgccc tgacagagat tctgcccgga ttaccgtcca tatcaagctc cgccatggcg
    10561 ggaatggtcg tcagcgtacg tgccagcctg ctccctaccc acacctgcac tgagtcaacc
    10621 tctgtctgtc ctgcagcggg accaacggaa aaaacgatcc tgtcggtgca gacataggac
    10681 gcattatcag caggtgacag cacctctatc agtcttttgg ccggtctcgg gtcactctct
    10741 gtggcaacag cagcatcttc cacggccttt gttccacgct tcccggcaga gccggaacat
    10801 gagatcacgg ataccagcac cagactcagc agtgagatat aaccaacaat tctcttcatg
    10861 ggttctgttc attttttgcc aaacagactg aaccggcgcc tttttatcct gcgcatcgcc
    10921 tcctcctccc tttcagtcca tgggtcaatc atgtggagaa cctcacccca tcccggaaaa
    10981 ccgccagcgt tcctgtcatc aatgaagatg tccacattga tcttcctcgg gttaccgtct
    11041 gtccactgtt cttccgggta attacggttc acggcataaa attcgattcc attctgactg
    11101 caaaagtcga cggcttcctg taactccttc ccctcacgga aggtccacag gatgagcagc
    11161 gcccccctct tctgaagctc cttcagggtg atgaaggcaa aaagcttctc tttgccgatg
    11221 gcgggatact catgttcaac aatggtgccg tcaaagtcaa ctgctatttt tatattggtc
    11281 aggtccctca tgatccgaag gcaaaaataa acaaataata attgccacta ttattgtatt
    11341 ttagcgatgc ctcaaaaaga gttatgataa aacatatccc gaacacactc acccttctga
    11401 accttatatg tggttttgcc gcaatagtct gtgtaataaa aggttataat gattatgcat
    11461 catactgcat catagtcgga atggtgtttg atttttcaga tggttttgca gccagagtgc
    11521 taaaggcata ttcagccctg ggaaaagaac tggacagcct tgctgacatg gtaaccttcg
    11581 gggtcgctcc cggctccatc gtattcaacc tcctaaccgc agggggcatg actgaactga
    11641 aagcctttgc cgttgcagtc atcatacctg ccgcctcagc gctgaggctt gccagattca
    11701 acaatgaccc ggagcagtca tcatcattca ggggattagc cacacctgca agcgccttca
    11761 cgatagtctc ggtgtcactc gccg
//
LOCUS       DAOPBK010000076        11754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5385_length_11754_cov_5.443542, whole genome shotgun sequence.
ACCESSION   DAOPBK010000076 DAOPBK010000000
VERSION     DAOPBK010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5385_length_11754_cov_5.443542"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PLA17_05920"
     CDS             complement(<1..574)
                     /locus_tag="PLA17_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPK84986.1"
                     /translation="MMNEKKTTADTCPAIILSAGFSERMGQPKALLKWNPSTTFIEKI
                     INEFCSFNCNPVICVINKSIEPDCSRLRVPSRVKFIITEHPESGRFSSVRTGINEVTE
                     ADFCFIHNVDNPFVNRDVISRLFALRDPDAWCSPVYAGKGGHPVLISSSVIRVIANTH
                     DLAITLADILNSFPRINMEMADDTPLRNINT"
     gene            complement(571..1794)
                     /locus_tag="PLA17_05925"
     CDS             complement(571..1794)
                     /locus_tag="PLA17_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84987.1"
                     /translation="MDDSTVIKWVKEKTGLGDNLNHETLKAWQREKFSSVAEYAAGRT
                     KFYASMIDPSAPLEEMPFTLPSDLAADPLAFLAIPLNEVVRVTTLAHSGTTALRKRVF
                     FSAADIERTIEFFAVGMSTMTRAGDRVSILISNPTENSLGSLLRTSLSRIGVEATIAP
                     AIRSVDEALKAAQGADCLVGMPGEMLYMCHAFPWLKPRSLLLAGDIVPPPLADTIREI
                     WQSDLYIHYGHSEFGYGCAVDCTHHQGLHTRDADLVFEVIDPVTGKASAPGEVGEIVI
                     TTLTESAMPLIRYKTGNLSRLIDSRCQCGGMTHMISPVEGRVSNNIPIGNGRHLNIYQ
                     LDNLIFANRSVRGFNAFLKNENGRITLDLVIESREKISPSLLREALFEDISLRVSYGN
                     ADPFRQRAKRRIKVS"
     gene            complement(1807..2445)
                     /locus_tag="PLA17_05930"
     CDS             complement(1807..2445)
                     /locus_tag="PLA17_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPK84988.1"
                     /translation="METDSEYSNQACERPGGLTITRRAIEFCGFPPGARIIDVGCGSG
                     LTVEFLREKCGLEATGIDINATGENSHLRAAQARQLPFEPYSADGVFMECSLSLFNDQ
                     EAALSECFRVLKPGGWLIVSDMYARGEAARLTGPLGRIERRETIENLVSSHGFTVKLW
                     EDFSQYLMAWWGQMIMDQKMESFFNSSGCSPESLKRVKAGYFLMIASKPVYR"
     gene            complement(2519..4555)
                     /locus_tag="PLA17_05935"
     CDS             complement(2519..4555)
                     /locus_tag="PLA17_05935"
                     /inference="COORDINATES: protein motif:HMM:NF040157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HPK84989.1"
                     /translation="MNTRSANYFVSGCMVCGSELVVKEGTEKVVCHYCGEPYVTHITC
                     EKGHYVCDSCHSSESVRLITSICRESRERDAALLMQAIRSHKSFPLHGPEHHILVPAV
                     ILTALRNNGADITEEQIITAVQRGRTVPGGSCGFMGVCGAATGAGIAYSILTGASPYD
                     PELRQAVQKSTAAVLEEIASYSAARCCQRDTWIALKKISELLEQNLGLNMPVSPIRCR
                     QFSLNKECIHENCPLRDPQITTTDGHGKTWSLCPVCLKRITADRIMNEGKVYLRKTCE
                     DHGTFQTLIWRGYYSFDEWIGNQHVQFSHAPQCPDNCGLCDDHLQKTCCVLLNVTDNC
                     NLGCTFCLANQQNGKKDPSLSEIYESLNNLMVAGKSLVQLSGGEPTTRADLPDIVRAA
                     RQAGAKYVQLNTNGIRLGNDPEYVKALADAGLSFVFMQFDGTDDSINVKLRNRPLLEA
                     KKRAIENCAAFNLGVTLVPTLVRGVNTNNIGDIIRFAVNSSPAVRGVHFQPVSFFGRV
                     PRLPSDDDRFTLDELVHEISLQTGGKIDPANLLPSRCDHPLCGFHGDFVIDSNHELFP
                     LSERGCSEGTGCCDPDSAERNREFVARRWQRPVEETGRETGRSGDLHDMEYFLSRVRT
                     HGFTLTAMAFQDAGNIDLARLQHCSFHVYDKGRMMPFCAYYLNSWPGVAGQPCK"
     gene            complement(4689..4883)
                     /locus_tag="PLA17_05940"
     CDS             complement(4689..4883)
                     /locus_tag="PLA17_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HPK84990.1"
                     /translation="MEERKKLICFKCNKELVPKKTDFSYLGHTFFTEIPKCPQCGLVY
                     LSEEIVKGRISEVEMQLEDK"
     gene            complement(4888..7119)
                     /locus_tag="PLA17_05945"
     CDS             complement(4888..7119)
                     /locus_tag="PLA17_05945"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPK84991.1"
                     /translation="MAFLHELVRDCLQDEKAFCTVKCPFNLDVRDFMGKLQQGRFNAA
                     YKTYQNTVGFPGIVSVLCPEPCRDVCALKEKGGSVSMRLLEAASMNFARNREPDQYNM
                     PPKSARIAVIGAGPSGMACALRLTTRKYKVTLFEKSGHIGGHLNDLPDADILIDDIRL
                     QFKYETLDLRLNSRVTDISVPGFDAIYVATGMHGDTFGLSLTTEGHYSTDRNGVFMGG
                     SVTGADVMQAIAQGLNAASVIEGFLKTGRMDHFEPPATETKLTYSAIKIIPSGAVRPE
                     NGKEYSREEARAEANRCLKCTCDACVRYSPLLSYFRKFPKKITEEVEVSINPSSLDGE
                     ATLATRLISTCNYCGLCREVCPKEIDTGEFLLRSHYTMREKGKMPWAFHEFFLRDMEF
                     STGEASLLLQPREEGEVKYLFFPGCQIGASDPRLVIRSYDFLLKNYPSTALLLGCCGA
                     PADWAGNAPAHKEVLKKIRSAWNDLGNPTIVFACATCNLMFRKHLPEMKGVFIYDLIA
                     RSDAIPAIPSIHGNKTASVFDPCAARYEDGLQETIRSIATGAGFRLEPLPMEGRLAEC
                     CSYGGQVSVAHPPYAEYTVKKVINRNTNPYITYCSNCRDTFARAGKETMHILDVVFGL
                     EQRPQPTVSERHENRLRLKAVLLKEYGNDNFNMEMKESKLTMSSGLREKLDTSMILES
                     DILTLIETCEQSGNKILDPENGSFIGHLQIGNMTFWAEYRVLDDGRYELINCYSHRMK
                     IEE"
     gene            complement(7267..10206)
                     /locus_tag="PLA17_05950"
     CDS             complement(7267..10206)
                     /locus_tag="PLA17_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPK84992.1"
                     /translation="MNKEESIMKFKKMWLNINGANRMFACNPEKDSLADVLRRMGLTG
                     TKVGCNVGVCGSCSVILNGKLTRACTKKMRNLDDYSKVITIEGIGQPNNLHPLQAAWI
                     VAGAVQCGFCTPGFIVSSYALLLENNNPTREEVREWFHKNKNVCRCTGYVQIVDAVMM
                     AAAVMRGEKTIEDFSLGHQEDGEYYGKPITRPTALAKVCGLADYGDDQELKMPAETLK
                     VAMVQPKVAHHARIIRIDTSEAEKMPGVERIITAKDIKEAGGTNIMAEGHFFERTTLT
                     VPTRHVLCEDKIFRYGDVVALVCADTKDHAREAAAKVKVEIEQLPEYLSYLDAVMPDA
                     QRIHPSTPNIFAQQPVLKGVGLEEPSKVAELIDSSEYSVEGSFYSTREPHLSIEGDSV
                     QAYFDEDGMLAIQCKSQGVYSSIGRIGSSVGVAKDKIRIIMNPTGASFGWSTNAGDLC
                     LAAAAAYITKKPIALSMSYEEHQHFSGKRRPCYSNGRASCDKDGKLVAVEYDYGMDQG
                     AYTFGGDDIISKPSRFAFFPYKVPNVAGLTRIAITNHNFGTAYRSYGSPQAYTLSESL
                     MDMLAEKAGIDPFEFRWRNIAREGDLNINSRPFRMYPMEDMMKLMKPHYDKAVKEARE
                     KDTPGVRRGVGLAWGGFNVSEGPTDNATVHLELNADNTITKYDTWQELGQGGDVGSLM
                     VTLEALKPLKLKPEQIKLIQSDTKICPDSGMSAGSRSHYMNGNATIAAANKMLDAMRK
                     PDGTFRTYDEMVKEGLPTKFEGKFANVVTPGLSRLDPNTGMGDPTPAFTYALNMAEVA
                     VDTKTGKTTVTRFVCVADVGRIGNIDAVNGQAFGGISHSIGFALSEDYDDVKKHSNIA
                     GSGVPYIKDIPDEIIVLYNDNYDKTGPFGSSGASEAFQASGHVAVLNAIYNACGVRVH
                     EMPATKEKVKAGLDILARGEKIEPQKKYFLGSDLYDELENIKANPVPFGGNDFFKPIG
                     GAGERFF"
     gene            complement(10218..10748)
                     /locus_tag="PLA17_05955"
     CDS             complement(10218..10748)
                     /locus_tag="PLA17_05955"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPK84993.1"
                     /translation="MLKDHLAEANELFRSGFNCSQAIVAVFSEEHGFPAETAIRMAYP
                     FGRGMGGCGCTCGALTGAMMVIGMKYETASQIGMKYGAAGQPEMKSGAAGQPEMKYGA
                     AGQDETDNKKLAREKTRRLIEFFENEHGTTLCNDLTGLDQRKLNGPELMAMLPHFHSR
                     CQKFLETVVTFLEEEL"
     gene            11265..>11754
                     /locus_tag="PLA17_05960"
     CDS             11265..>11754
                     /locus_tag="PLA17_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPK84994.1"
                     /translation="MEERINGTLDKLEIKKINSGICTGTSWFETHGPVIMSEDPSTGR
                     EIAAVQSASYDDYDLMIKGAERAFLEWRRCPAPKRAEIVRQIGLKLREYKEDLGRLIS
                     IEAGKILQEGMGEVQEMIDICDFALGQARMMNGVMLRSERPDHKMYDQYHPLGIVGIV
                     TSF"
BASE COUNT         2810 a         2942 c         2810 g         3192 t
ORIGIN      
        1 gtgtgtttat atttctcaag ggcgtgtcat cagccatttc catgttaatt ctggggaaag
       61 aattaagaat gtccgcaagc gttattgcaa gatcatgagt gttggctatc actctgatga
      121 ctgaggagga gatcaggacg ggatgacctc ccttgccggc ataaacaggt gagcaccacg
      181 catccgggtc acgcaaagcg aagagtctgc tgatgacatc ccggtttaca aaaggattat
      241 caacattatg aataaaacag aagtcggctt cagtgacctc gttgatacct gttcttacag
      301 aagagaacct tcctgactca ggatgctcag ttattataaa cttcacgcgt gaagggaccc
      361 tcaatctact gcaatcgggt tctattgact tgtttatgac acatatcacg gggttacagt
      421 taaagctgca gaactcgttg attattttct caatgaaagt ggttgagggg ttccacttca
      481 acagtgcctt tggttgtccc attctttccg agaacccggc agaaagaata attgctgggc
      541 aggtatctgc cgtagttttt ttctcattca tcatgatact tttattcttc ttttggctct
      601 ttgcctgaag gggtcagcgt tgccatagct cacccttaaa ctgatgtcct caaaaagagc
      661 ctctctcagc agagacggac tgatcttttc cctggattct atcacgaggt caagggtaat
      721 ccttccgttt tcatttttca ggaatgcatt gaatcctctt acagacctgt tggcaaaaat
      781 caggttatca agctggtata tattcaggtg cctgccgttt cctatcggga tattgtttga
      841 tacacgtccc tcaaccgggg agatcatgtg tgtcatgccg ccacactggc accgggagtc
      901 tatcaaacgc gagaggttgc cggttttgta tcgtatgagc ggcatggctg actcagtcag
      961 tgtggtaatt acaatctctc ccacttcgcc cggggcggaa gctttcccgg taaccggatc
     1021 aataacttca aaaacaaggt ctgcatcacg tgtatgaagt ccctggtgat gagtacaatc
     1081 aaccgcgcat ccgtaaccga actccgaatg accgtagtgg atataaagat cgctttgcca
     1141 gatctccctg atggtgtcag ccagcggtgg aggcacaata tctccggcga gaagcagtga
     1201 gcggggttta agccatggga aggcatggca catgtagagc atttcaccgg gcatacctac
     1261 caggcagtca gcaccctgtg cggccttcag tgcttcgtcg actgatctga tggcgggagc
     1321 aatagttgct tccacaccta ttcttgagag gcttgtccgc agaagtgaac cgaggctgtt
     1381 ttcggttggg tttgagatca gaatggagac gcggtcaccc gcgcgggtca tcgtgctcat
     1441 gccaacggca aaaaactcta tggttcgctc aatatcggcg gctgaaaaaa agaccctttt
     1501 tctgagagca gtggtgcctg aatgtgccag cgtggttacc ctgaccacct cattcagcgg
     1561 tatggcgagg aaggcaaggg ggtcagccgc cagatcggaa ggcagtgtaa agggcatctc
     1621 ctccagtggt gccgaggggt caatcatact ggcatagaac tttgttctgc ctgcagcata
     1681 ctctgcaact gaagagaatt tttctctttg ccatgccttc agtgtctcat gattcagatt
     1741 gtcaccgagg cctgtctttt ccttaaccca ttttattaca gttgagtcat ccatctgaat
     1801 gaaatttcat ctataaaccg gtttggaagc tatcatcagg aagtagccgg cttttacccg
     1861 ttttaatgat tcagggctgc agccggagct gttgaagaat gattccatct tctgatccat
     1921 tatcatctgt ccccaccacg ccatgaggta ttgcgagaaa tcctcccaca gctttacagt
     1981 aaaaccatga gatgaaacaa ggttttcaat tgtctcacgt ctctctatcc ggccaagagg
     2041 gccggtgagc ctggccgctt cgcctctggc atacatatca gaaacaatca gccatcctcc
     2101 gggtttcaga acacgaaaac actcactcag cgcggcttcc tgatcattga agagtgagag
     2161 gctgcactcc atgaagaccc cgtctgcgga ataaggttca aaggggagtt gccttgcctg
     2221 tgcagcacgt aaatgactgt tttcaccggt agcattgata tctatgcctg tcgcttcaag
     2281 tccgcacttc tccctgagga attcaacagt gagaccgctc ccgcatccca cgtcaataat
     2341 ccttgccccg ggggggaacc cgcagaattc gatagcccgc ctggtgattg tcagccctcc
     2401 cgggcgttca cacgcttgat tgctatattc tgaatccgtt tccatcgggg aaaaaccaaa
     2461 taaccctgac tggcaaattg tgattactgt atcgtgattc cgtctccacc ggatttgctt
     2521 atttgcatgg ctgacctgca acaccgggcc agctatttag gtaataggca cagaatggca
     2581 tcatccgtcc cttgtcatag acatgaaagc tgcagtgttg caggcgtgca aggtcaatat
     2641 tcccggcatc ctggaaagcc attgcagtca gggtgaatcc atgtgtcctg accctgctga
     2701 ggaagtattc catatcatgc aggtcacctg agcggccggt ttctcttccg gtttcttcca
     2761 cgggtctctg ccatcttctg gcaacaaatt cgcggttacg ctctgcagaa tccgggtcac
     2821 aacaacctgt cccttcactg cagcccctct cagagagagg aaacagttca tgattgctgt
     2881 caatcacaaa gtcgccatgg aatccgcata gaggatggtc acaccgtgaa gggagcaggt
     2941 ttgcaggatc aatctttcct ccggtctgga gggagatctc atgaaccagt tcgtcaagcg
     3001 tgaaccggtc atcgtcagaa ggcagccgtg gtacccgtcc gaagaagctc acgggctgga
     3061 aatgcacacc tcgcacggca ggggaggagt tcacggcaaa cctgataata tcacctatgt
     3121 tgttggtatt aacccctctg accagcgttg gcaccagcgt cactccaagg ttgaatgcgg
     3181 cacagttctc aatggctctc ttctttgctt caagcagggg gcggtttctg agtttcacgt
     3241 ttatagaatc atctgtaccg tcaaactgca tgaatacaaa agagagacct gcatcagcca
     3301 gtgcttttac atattccggg tcattcccaa gcctgattcc gttagtgttc agctggacgt
     3361 actttgctcc ggcctgcctt gcagcccgaa cgatatcggg gaggtcagcc cgtgttgttg
     3421 gttcacctcc gctgagctgg acaaggcttt tcccggcaac catcaggtta ttaagtgact
     3481 cgtaaatctc actcaatgac ggatctttct ttccgttctg ctggttggcg aggcagaagg
     3541 tgcaaccgag gttacagtta tcggtaacat tcagcagcac gcagcatgtt ttctggagat
     3601 gatcatcaca aagtccgcag ttatcggggc actgaggagc atgggagaac tgcacgtgct
     3661 gattgccgat ccattcatca aagctgtagt aacctctcca gatcagggtc tggaaggtgc
     3721 catggtcttc acaggttttc cgtaagtaaa cttttccttc attcatgatc ctgtctgccg
     3781 ttatcctttt caggcatacg gggcagaggc tccatgtttt gccatggccg tcagtagtgg
     3841 tgatctgagg atcccggaga gggcaattct catgtatgca ctctttattg agactgaatt
     3901 gccggcaccg gatgggtgat acaggcatat tgagacccag gttctgttcg aggagttcag
     3961 atattttttt aagcgctatc catgtatccc tctggcagca gcgggctgca ctgtatgatg
     4021 caatttcttc aagcactgca gctgttgact tctgtactgc ctgcctgagt tccggatcat
     4081 agggcgaggc tcctgtaaga atggaataag caatgccggc accggtagcg gcgccgcata
     4141 cacccatgaa tccgcatgat cctccaggaa cggtgcggcc tctctgcaca gccgttatta
     4201 tctgttcctc agttatatct gcaccattgt tccggagtgc tgtaagaatc actgccggaa
     4261 caaggatatg atgctcgggt ccgtgtaaag ggaaagattt atgtgatctg atagcctgca
     4321 tcagcagggc agcatccctc tcccggctct ccctgcagat ggaagtaatt aatctgacgg
     4381 attctgaaga atggcagcta tcgcatacat agtggccttt ttcgcatgtt atatgagtga
     4441 catacggctc tccgcagtaa tgacagacca ctttttccgt gccctctttc actaccagtt
     4501 cactcccgca aaccatgcat cctgagacga aatagtttgc actccgcgta ttcatgttat
     4561 tcgtccttta gttgcatttc aagatcgtca gatttcatcg aatcctccaa gtcttcagca
     4621 tgttacttgc cctccagttg taattcaact tcgcctgatt ttatcgaacc ctccaagtct
     4681 tcagcatgtt acttgtcttc cagttgcatt tcaacctctg atatacggcc ctttacaatt
     4741 tcttcagaga gatagacaag accgcattgg gggcatttag ggatctctgt aaagaaggta
     4801 tgacccagat atgagaaatc ggtttttttc ggtaccaatt ccttattgca tttaaagcat
     4861 ataagcttct tcctctcttc catagcctta ttcctctatt ttcatccggt gactgtaaca
     4921 gttgatcagc tcataacggc cgtcatcaag tacccggtat tctgcccaga atgtcatatt
     4981 gccaatctgg agatgaccga tgaatgaacc attttcagga tcaagtatct tgttccctga
     5041 ctgttcgcag gtttcaatca gggtaaggat atccgattca agtatcatgc tggtgtcaag
     5101 cttctccctg agaccggagg acattgtcag tttggattct ttcatttcca tattgaaatt
     5161 gtcatttcca tactctttaa gcaacacagc cttcagacgc aatctgtttt catggcgttc
     5221 cgacactgtt ggctgaggcc tttgctccag gccgaagacc acgtcaagga tatgcattgt
     5281 ttccttcccg gcacgggcaa aagtgtcacg gcagtttgag caataagtta tataaggatt
     5341 ggtgtttctg tttataacct tttttaccgt gtactctgca tagggtgggt gtgccacaga
     5401 gacctgtcct ccgtagctgc agcattctgc aagccggccc tccattggga gtggttcaag
     5461 cctgaaaccg gccccggttg ctatactcct gattgtttcc tgcagaccat cctcatatct
     5521 tgccgcacag ggatcgaaaa ccgaagccgt tttgttcccg tggatggaag ggatggcggg
     5581 gatggcatcg ctgcgggcaa taaggtcata tataaataca cctttcatct ccggcagatg
     5641 ctttctgaac atcaggttgc aggttgcaca tgcaaataca atggtcgggt ttccaagatc
     5701 attccaggcc gaccttattt ttttcaatac ctctttgtgt gccggagcat tgcctgccca
     5761 gtcggccggt gctccgcaac acccaagcaa cagtgcggtg gaggggtagt tcttcaacag
     5821 gaaatcataa ctccggatta caagcctggg gtcagaagct ccaatctggc atccggggaa
     5881 gaaaaggtac ttcacctcac cctcttcacg gggttgaagc aggagagagg cttctcctgt
     5941 tgagaactcc atatctctga ggaagaattc atggaacgcc catggcattt tgcctttttc
     6001 ccgcattgta taatgacttc tcagcaggaa ctcgccggta tcgatctctt tcgggcatac
     6061 ctccctgcaa aggccgcagt agttacatgt tgagataagg cgtgttgcca gggttgcttc
     6121 cccgtcaagg gagctgggat tgatgctgac ctcaacctcc tcggtaattt ttttcggaaa
     6181 ctttctgaaa tagctcagca gcggcgaata acggacgcag gcatcgcagg tacatttcag
     6241 acaacggttt gcctctgcac gggcctcttc ccttgaatac tcttttccgt tttcaggcct
     6301 gacagccccg gaggggatga tcttaattgc actgtatgtc agctttgttt ctgttgccgg
     6361 cggttcgaaa tgatccatcc ggccggtttt cagaaaacct tctattaccg aagcagcatt
     6421 gagtccctgc gctattgcct gcatgacatc agcccccgta acagagcctc ccatgaatac
     6481 cccgttgcgg tctgttgaat aatgcccctc agtggtaaga gagagtccga aagtatctcc
     6541 atgcatgcct gtggccacat aaattgcgtc aaagccaggc acggagatgt cagtcaccct
     6601 gctgttcagt ctgaggtcaa gggtttcata tttgaactgc agcctgatat catcaatcag
     6661 aatatctgca tcagggaggt cattgagatg ccctcctata tgtccgctct tttcaaacag
     6721 tgtgaccttg tatttcctgg tggtcagtct cagggcgcat gccatgcctg aggggccagc
     6781 ccctataaca gctatccggg ctgacttggg aggcatattg tactgatccg gctccctgtt
     6841 gcgggcaaaa ttcattgatg ctgcttcaag caacctcatt gaaacagacc ctcccttttc
     6901 cttcagcgcg cagacatccc tgcagggttc agggcagagc acggatacaa tgccgggaaa
     6961 cccgacggtg ttctggtagg tcttgtatgc ggcattaaac ctgccctgtt gcaacttccc
     7021 cataaaatca cgcacatcga ggttaaaagg gcacttcacc gtacagaatg ccttttcatc
     7081 ctgcaaacaa tccctgacaa gttcgtgtaa gaatgccatt ttcccgtgga ggcaacatga
     7141 ttaccccata aacttactat aaaatcaata atgaatataa ctttctttcc ggcccgtgtt
     7201 ccctgccttg cgaaaatgga gagaagcccc gcgaaagggg gcttctccgg tattggtata
     7261 acataatcag aagaatcttt ctcctgctcc gcctatgggt ttgaagaagt cattaccgcc
     7321 aaatggaacg ggattggctt taatgttctc cagttcatca taaaggtcag aaccaaggaa
     7381 atatttcttc tgcggctcaa tcttttctcc gcgtgcaaga atatccagac ctgccttgac
     7441 cttctccttc gttgcaggca tctcatgtac cctgactccg caggcattat aaatggcgtt
     7501 cagcacagca acgtggcctg atgcctggaa tgcctcagag gctccggatg agccgaatgg
     7561 tccggtcttg tcataattgt cattgtagag aacgataatt tcatcaggaa tatccttgat
     7621 ataggggaca ccgcttccgg caatgtttga atgctttttc acatcatcat aatcttccga
     7681 gagggcaaag ccgatgctat gggatattcc tccgaaagcc tggccattca ctgcgtctat
     7741 attccctatt ctgccaacgt cagcaacgca gacaaaccgt gtaacggtgg tttttccggt
     7801 ttttgtatcc actgccacct cggccatgtt gagagcataa gtgaatgcag gtgtcgggtc
     7861 gcccatcccg gtattcgggt caaggcggct caggcccggg gtaacgacat ttgcaaattt
     7921 gccttcaaac tttgttggta acccttcctt aaccatttca tcataggtcc ggaaagtgcc
     7981 gtcaggcttt ctcatcgcgt caagcatctt atttgcagcg gcaatagttg cattgccgtt
     8041 catatagtgt gagcggctgc cggctgacat tccgctgtca gggcagattt tggtgtcact
     8101 ctgaataagc ttgatctgtt ccggcttgag ctttaatggt ttaagagcct cgagggtcac
     8161 catgagtgat cctacgtcac ctccctgacc gagttcctgc caggtgtcat acttggtaat
     8221 ggtgttatca gcgttcagtt cgaggtgaac agttgcatta tccgttggcc cttcgctgac
     8281 gttgaaacca ccccatgcaa ggcctacacc acgacgaacc ccgggtgtat ccttttccct
     8341 ggcttccttt actgccttgt cataatgagg cttcataagc ttcatcatat cctccatcgg
     8401 atacatcctg aatggcctgc tgttgatatt gaggtcgcct tcacgtgcaa tattcctcca
     8461 tctgaattca aacgggtcta ttcctgcctt ttctgcaagc atatccatga gtgattcact
     8521 gagggtatat gcctggggtg agccgtaact acggtatgct gttccgaagt tatggttggt
     8581 gatggctatg cgtgtgagac cggctacgtt tggcacctta taggggaaga aggcaaagcg
     8641 cgagggcttg gaaatgatat catcacctcc gaaagtgtag gcaccctggt ccatgccata
     8701 atcatactca acagccacca gttttccatc cttgtcacat gatgcacggc cgtttgagta
     8761 gcagggacgg cgtttgccgg agaagtgctg atgttcctcg taactcattg agagcgcaat
     8821 gggtttcttt gtgatatatg cagctgcagc agccaggcac aggtcaccgg cattggttga
     8881 ccatccgaaa ctggctcctg tgggattcat gattatcctg atcttgtctt ttgcaacacc
     8941 taccgagctt ccgatacgtc cgattgagga gtatacaccc tgtgacttgc actggattgc
     9001 gagcatgccg tcctcgtcaa agtaagcctg aaccgagtcg ccttcaatcg agaggtgcgg
     9061 ctcacgggtt gagtaaaagc tcccttcaac actatactcc gaactgtcaa tcagttccgc
     9121 aacttttgac ggctcttcca gtcccacacc cttaagcacc ggctgctggg cgaagatgtt
     9181 tggtgttgac gggtgaatgc gctgggcatc cggcatgacc gcatcaagat aactcagata
     9241 ttcaggcagt tgttcaatct caaccttaac ctttgctgct gcttcacggg catggtcttt
     9301 tgtatcagcg catacgagtg ccaccacgtc accataacgg aatatcttgt cttcgcacag
     9361 gacgtggcgg gtggggacgg tgagcgttgt gcgttcaaag aaatgaccct cggccatgat
     9421 gttggttcct cctgcctcct ttatgtcttt ggctgtgatt atgcgttcaa ccccaggcat
     9481 cttctcagct tcagaggtgt ctatacggat gatcctggca tgatgtgcca ccttcggctg
     9541 gaccattgct accttgagtg tttcagccgg catcttcagc tcctgatcat caccgtaatc
     9601 agccaatccg cataccttgg ccagagccgt cggacgggtt atcggcttcc cgtaatattc
     9661 gccatcttcc tgatgaccta atgagaagtc ttcaatggtt ttttctcctc tcatcaccgc
     9721 ggctgccatc atgacagcat caacaatctg gacgtacccc gtgcaacggc acacattctt
     9781 gttcttatga aaccactccc tgacctcttc acgggtaggg ttgttgtttt caagcagcag
     9841 ggcataagat gatacgataa atcccggggt acagaatccg cactgaacgg ctccggcaac
     9901 aatccatgct gcctggagag gatgaagatt attcggttgc ccgattcctt caatggttat
     9961 taccttgctg taatcatcca gattgcgcat cttttttgta caggcacgtg tcagcttgcc
    10021 attgagaatt accgagcacg agccgcagac tcccacattg cagccgacct tggttcccgt
    10081 aagtcccatc cgtctgagca catcggcaag tgaatccttt tcaggattgc aggcaaacat
    10141 cctgttggct ccgttgatat tgagccacat ctttttaaac ttcataatag actcctcctt
    10201 gttcattggt tatttatcta taattcttct tcaagaaatg ttacaactgt ttccaggaat
    10261 ttctggcatc tgctgtgaaa atgaggaagc atggccataa gctcaggtcc gttaagcttc
    10321 cgctggtcaa gacctgtaag atcattgcag agggttgtgc catgttcatt ttcgaagaac
    10381 tcaatcagcc tccgggtctt ctcacgggca agtttcttgt tgtcagtttc gtcctggccg
    10441 gcggctccgt atttcatttc gggctgaccg gcagctccgg atttcatttc gggctgaccg
    10501 gcagctccgt atttcatgcc tatctggctg gcggtttcgt atttcatgcc gataaccatc
    10561 attgcaccgg tgagtgctcc gcaggtacat ccgcagccac ccatccctct tccgaacggg
    10621 taagccatcc ttattgcagt ctctgccgga aaaccatgct cctcactgaa gacagcaact
    10681 atggcctgtg aacaattgaa cccagaccgg aataattcat ttgcttctgc cagatgatct
    10741 tttagcatat tgttcgacct gcaaattaat acataacgaa aaccataaaa aaagcggcaa
    10801 ccaaccgcct tgctgttatc ctacccgtaa aaaaccaatt tttgctgagc aggcataaaa
    10861 gtcttattta cagtattatt atctatagtt accggcattt atggcagtag tcaattctca
    10921 ttaataaaag tagtgtttgc cggagcaaaa aagcgtgaga aaaatcagct atttggtttt
    10981 cttgtttttg aagcagtgga acaggagagt taaggtgaca gaactcctga cataacatat
    11041 cagtaaaatg gtaaaaagct gcctgtttcc aatagacaat aatcaatata ataactcaat
    11101 acgcacattt tcactagtat atatcgaggc aaaatagttg cagcccatat atcaaccgga
    11161 gaaaaagcca gctggaattg cgaatatgat ttttgttatg aaaaaaaagg catatcggtc
    11221 ttatgtttga aacctcatta tagaatttta aagaaacaat agttatggaa gaaagaatta
    11281 atggtaccct ggacaagttg gagattaaga aaatcaatag tggaatatgt accggaacca
    11341 gttggtttga gacgcatggc ccggtgatta tgtcagagga cccgtccacc ggtagagaaa
    11401 tagcagctgt acaaagtgcg agttatgatg attatgatct gatgatcaaa ggggcggagc
    11461 gtgctttcct ggagtggcgc aggtgcccgg ctccgaagag ggcagagatt gtgaggcaga
    11521 taggcctcaa gctgcgcgag tacaaggaag atctgggcag gctcatcagc atcgaagccg
    11581 gcaagatcct gcaggaagga atgggtgagg ttcaggagat gattgatata tgtgattttg
    11641 cacttgggca ggccaggatg atgaacgggg tgatgctgag gtcagagagg cctgatcaca
    11701 agatgtacga ccagtatcat ccgcttggca tagttggcat tgtaacatca ttca
//
LOCUS       DAOPBK010000077        11642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5449_length_11642_cov_4.639941, whole genome shotgun sequence.
ACCESSION   DAOPBK010000077 DAOPBK010000000
VERSION     DAOPBK010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5449_length_11642_cov_4.639941"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2128
                     /locus_tag="PLA17_05965"
     CDS             <1..2128
                     /locus_tag="PLA17_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84995.1"
                     /translation="DTFRFFPEYMLTRAKTFDMKPDSDGRFPVLKAQDVDIQWLTDED
                     EWYAMNSKGKEFSMFGNNTSMDGTLILQPDKMKGQGEVNTADARITSETFSFGPMSFE
                     ADTSDYYLRALKGYGYGFVASDVNTHVDFATQKASFRLNTDSSFVVLPGIEYISKMTS
                     FEYDMEKKVMDMFQEGRESVPLMSPQELLEVPHGLKEKPTFLSTNNMKDTLKFQSGSA
                     SYFLQDEYLMVKDVNYIPVADALIQPGEGVLYIERGAQIRPTESAVVAVNNRHLIHSA
                     KLKIESSANYSGSGKYDYISADGSSQVIEFSDIRVDTMATKATGHIPATMNFTLSPAF
                     TYSGDVRLRSDSEQLFFTGAAGIVTDCRNIRSKQVKFRAHIDPANVLIPVTDKARDLD
                     DNLIFSGSFVTLDSAGVYGAFLSEKYSWSDTPLLNAEGFLFHDKGIDRYRIASMDKLA
                     DLRRHGDMVTFDRNMCLLLSEGKINFGVNYGLLRLNSSGAVTHNTDSSRVEVRSILGF
                     SFHFNAEALKTMAADIRTNPTLKTVNLSSEFNQKAMRDLLGEETAKKLNEELQLFGVV
                     RSLPKEYTSQLLLNDVTLQWNPYSLSFISKGKIGIGFIGDQPMNVYVDGWVELQRKRS
                     GDLLDIYLKASDNTWYWFSYFRGILMSYSSNPEYNDILTNTKEKLRRDPNASARIDFE
                     YMPGVPDRLRSFLRRMESGGLGFDEY"
     gene            complement(2176..2685)
                     /locus_tag="PLA17_05970"
     CDS             complement(2176..2685)
                     /locus_tag="PLA17_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPK84996.1"
                     /translation="MRIGVISDTHGWVDPAVYEHFASVDEIWHAGDAGNAGVIAELEA
                     FRPLRAVWGNIDGFDVRRVTKEHLFFSAGSKNVLMIHIGGYPGRYAPRALELIRELKP
                     DIFVCGHSHIAKVIYDRSFGMLCINPGAAGRTGIHKVMTMLRFTIDGANISDMEVIEF
                     GSRGGGQYQ"
     gene            2748..3452
                     /locus_tag="PLA17_05975"
     CDS             2748..3452
                     /locus_tag="PLA17_05975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPK84997.1"
                     /translation="MQIFYCEDISGKKALFSREESGHCLRVLRMRRGDTLTFTDGKGN
                     LYNGVIDTDDPKGMSVTITSSKNDPTPRNYRLNVAISPLKNEERLEWFIEKAVETGID
                     VITPVICSRSEKKSIRRKRAEGIMISAMKQSGRCILPGLEEPESAEEFIRRPAKEKRL
                     IATCDSSFDRIAITEAFNRGEDVTILIGPEGDFTPAEVQAAISAGYVPVHIGTGRLRT
                     ETAGIAACCSVYLANI"
     gene            complement(3669..3854)
                     /locus_tag="PLA17_05980"
     CDS             complement(3669..3854)
                     /locus_tag="PLA17_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK84998.1"
                     /translation="MQKKSFIIRIDRDKLSAIEKWASDEFRSTNGQIEWILDQALRKS
                     GRLRDKRNKTDQNKDEK"
     gene            complement(3857..4711)
                     /locus_tag="PLA17_05985"
     CDS             complement(3857..4711)
                     /locus_tag="PLA17_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPK84999.1"
                     /translation="MKEEKIFRASSGYLFLVITILALTATVFSFIGEIYWLGCILILV
                     DIFLLAGLMVVNPNESMVMVLFGAYRGTVKKNGFWWNIPFYSRKRISLRARNFDSEPI
                     KVNDKNGNPIKIGLVLVWKVEDTYKAAFDVDKYEHFVLVQSDAALRKLAGLYPYDNWE
                     GHEHEMTLRSGGEEVNDELEKEIRERLMIAGINVIEARINYIAYSEEIAGAMLRRQQA
                     AAVVAARFKIVEGAVSMVEMALEQLSDKNIIELDEEKKAAMVSNLMVVLCGDKDPNPI
                     INTGTLHH"
     gene            4799..5596
                     /locus_tag="PLA17_05990"
     CDS             4799..5596
                     /locus_tag="PLA17_05990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK85000.1"
                     /translation="MHERHTDRELYFREQSQTTSKYVIPFISGVKQVTSSLSVLEIGC
                     GEGGNLLPFLRAGCARVTGIDISEYKIGNARRFYSSIPGGESMEFIAADIYDCADVGK
                     FDLVMMRDVLEHIHDHARFIGFVKRLLARDSVLFIAFPPWNNPFGGHQQVCDSPVLSK
                     LPWIHLLPRGIYRWLLRVAGEESGKIEGLLEIRDTGINTGRFEKIIREEGFSVLKKSL
                     WFINPNFEVKFGLRPRLLPGFPGAIPFLRNFLATTCYYIISMPRQEP"
     gene            complement(5568..7601)
                     /locus_tag="PLA17_05995"
     CDS             complement(5568..7601)
                     /locus_tag="PLA17_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85001.1"
                     /translation="MKKVIFLMVMVLTACSTPAKKGGETASAFIGETAVTSAIDRIMG
                     EQAAADRLLVEKGVQHAASLWREEDGTAEEFVDFVSANYISDPAKRKSVFIKISNYLE
                     SLAGNMNEVTIDLRKVLDETGGEIDEIDRMFGNYSVGSHLQSDFYDNKIAFVIALNFP
                     YYTLEEKDNLGAGWSREQWAMARLGDQFVSRVPAELNQQANTALGNADMYIAEYNIHM
                     GKLRTDDGRQLFPDDMVLLSHWNLRDEIKSNYADRETGNEKQEMVYRVMERIIRQEIP
                     EKVINNPGYEWAPYSNKVTKDGEAADAPAEPDTRYSHIISVFNALKATDPYNPGMNTA
                     MLRSFSGGMEISEEDAAALFREYLSSPQLRQVAEIIKERLGRDLRPYDIWYDGFKSRS
                     SISESLLTAKTSALYPDAAAFRAGLPAILVKLGWSPERARYLADKIVVDPARGSGHAW
                     GAAMKGAVSHLRTRISDKGMDYKGYNIAVHEFGHNVEQTISLYDVDYYTLSGVPNTAF
                     TEATAFLFQDRDLMLLGINDANPEKEKLQTLDAAWSLMEIMGVSLVEMGVWNWLYENP
                     EATPAQLSDKTVDIAVEVWNNYFAPVLGVNDSPLLAIYSHMVSTPLYLANYAYGQIIQ
                     FQIEEQIKGRKFSDEVDRMYSLGRLTPQHWMSQATGTKISARPLLRALDEALK"
     gene            complement(7618..9291)
                     /locus_tag="PLA17_06000"
     CDS             complement(7618..9291)
                     /locus_tag="PLA17_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HPK85002.1"
                     /translation="MMKRLIVCFVAVMTMLWSCTSKREKMENRMRDFIASYEAAVIPL
                     YKESALSSWDANVNGTDEAWARNEKASLAYISYFTDKEAFAELKELKESGKVKDPVLA
                     RQLEVIYNQFLSGQVDTSLLAEQVRMETEITKKFNSFRADVNGKMLSDNEVEDILRNS
                     TNSGELQAAWEGHKKIGPVVADDVIKLVRHRNRIAQELGFGNYHQMSLKLSGQDPEEV
                     TRIFDELDSLTEGTFATLKGEIDDYYSKRYGIPAADLMPWHYQNRYFQEAPEIYSVDF
                     DSYYKDKDPVKLATTYYASIGLPVDEIIARSDLYEKPGKNQHAFSTDIDREGDVRTLD
                     NIRPDSYWMNTIMHELGHGVYSYYNDRSLPFAIRDAAHTFTTEAVANFFGRYATDPAW
                     MHFMGVIDEKEAERINEEAFNAFRLQQLVFSRWAQVMYRFEKAMYENPDQDLNQLWWD
                     MVEKYQMMKKPAGRDMPDWATKIHVALYPCYYHNYLLGELLASQFSAFITNSVVRGNT
                     GEDMKAMGEFFKTKVFMPGMRYEWNEMIEKATGEKLTARHYAAQVIGEE"
     gene            complement(9522..9707)
                     /locus_tag="PLA17_06005"
     CDS             complement(9522..9707)
                     /locus_tag="PLA17_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85003.1"
                     /translation="MDKNSTLLLSYFEITNTPSDLIADAFGIDNALSVLLPDMEDSPS
                     DEVIASLFEKIRKNKVN"
     gene            complement(9760..10347)
                     /locus_tag="PLA17_06010"
     CDS             complement(9760..10347)
                     /locus_tag="PLA17_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK85004.1"
                     /translation="MKSKMTDYELITSFMNGNEKSIELLILRHKSKVYSYISLYIRNR
                     DLADDIFQDTFLKVVQSIRSGRYQDDGKFISWVMRIAHNLIIDHFRHEKQMGIVFNDD
                     YETDLFNSKKLTDDNVEDIIVRRQVMRDVRRLINGLPEDQREVVIMRHYAGMSFKEIA
                     DMTGVSINTALGRMRYALINMRKLMVEKNITLSIG"
     gene            10543..>11642
                     /locus_tag="PLA17_06015"
     CDS             10543..>11642
                     /locus_tag="PLA17_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HPK85005.1"
                     /translation="MKGNIEIKGARVHNLKDISLSIPRNSLVVITGVSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGRMNKPDVDFINGIPPAIAIEQKVNTRNPRSTVGTS
                     TEIFDYLRLLWARVGRTISPVSGREVRRHTVDDVVNYLAALTPGTRVVLTAPIAVPDN
                     VDPAEYIKFLIRQGYTRISTEKGVIRLDDAGTTELMTEMRSTELIIDRFAAGDSPEEL
                     SRAADSVQTAFLTGRGEINLTVTDEETTRTVRFSDRFEEDGIAFEEPTEHLFSFNSPL
                     GACPLCNGYGKIIGIDESLVIPDENLSVYDNAIACWRGETMKRWKERLIYSADKFGFP
                     IHKPWYKLTPEQKELVWNGNRYFFGLNRFFQHL"
BASE COUNT         2970 a         2884 c         2737 g         3051 t
ORIGIN      
        1 cgacacattc aggttctttc ctgaatacat gctgacaagg gcgaaaacct tcgatatgaa
       61 gccggattct gatggcaggt tcccggtcct taaagcacag gatgtggata tacagtggct
      121 cactgatgag gacgaatggt atgcaatgaa cagtaagggc aaggagttca gcatgttcgg
      181 aaacaacacc tccatggacg gtacccttat ccttcagccc gataagatga aaggtcaggg
      241 tgaagtgaat actgctgatg cccggatcac ctctgagact ttctccttcg gaccgatgtc
      301 attcgaggct gacacttccg attactacct cagggctctt aaaggctacg gatacggttt
      361 tgtggccagc gatgtcaata ctcatgtcga ttttgccacc cagaaggcat cattccggct
      421 taataccgat tcatcatttg tggtgctgcc gggaatagag tacatatcca aaatgaccag
      481 ctttgagtat gacatggaga agaaggtcat ggatatgttt caggagggca gggagtctgt
      541 acccctcatg tcacctcagg aattgctgga agtacctcat ggcctgaagg agaaacccac
      601 attcctttca acaaacaaca tgaaggatac cctgaagttc cagtcagggt cagcatcata
      661 cttcctgcag gatgaatacc ttatggtgaa ggatgtcaat tatattcccg ttgctgatgc
      721 tctgattcag ccgggcgaag gagtgcttta catagaacgt ggagcgcaaa tcagacctac
      781 tgagagcgcg gtggtcgccg tcaacaacag gcacctgata cactcggcaa aactgaagat
      841 agaaagcagc gcgaactaca gcggatcggg caagtatgac tatataagcg ctgacggcag
      901 cagccaggta attgaattca gcgatatcag ggttgacacg atggccacca aagccaccgg
      961 ccacataccg gcaaccatga acttcacgct cagtccggcc ttcacctatt ccggtgacgt
     1021 gagactgagg tctgacagtg agcaactctt tttcacaggc gctgccggaa tcgtgaccga
     1081 ctgcaggaac atcagaagca aacaggttaa gttcagggct cacattgacc cggcaaatgt
     1141 gcttattcct gtcactgaca aggcacgtga ccttgatgac aatctcattt tttccgggag
     1201 tttcgtgaca cttgattcgg caggtgtcta tggcgccttt ctttcagaaa agtattcgtg
     1261 gagtgatact ccgctgctga atgccgaagg cttcctgttc catgacaagg gcattgacag
     1321 gtaccgtatc gcctccatgg acaagcttgc tgatctgcgc agacacggtg acatggtgac
     1381 atttgacagg aacatgtgcc tgcttctgag tgaaggaaag atcaatttcg gagttaacta
     1441 cggcctgctc aggcttaaca gttcaggggc cgtcacacac aataccgact catcaagagt
     1501 ggaagtgcgt tcaatactgg gattctcatt ccatttcaac gccgaagcgc ttaaaactat
     1561 ggctgccgat atacggacca atccgacact caaaacagtt aatctctctt cagagttcaa
     1621 ccagaaggcc atgcgtgatc tgctgggcga agagacagct aaaaagctca acgaggagct
     1681 tcagctcttt ggtgtggtgc gctcactacc gaaggagtac acctctcagt tgctcctcaa
     1741 cgatgttaca cttcagtgga atccctattc gctctctttc atatcaaaag gaaaaatcgg
     1801 aataggattt atcggtgacc agcccatgaa tgtctacgtt gacggatggg tggagcttca
     1861 gaggaagagg tcgggtgatc tgctggatat ctaccttaag gccagcgaca acacctggta
     1921 ctggttctca tacttcaggg ggatactgat gagttattcc tcaaacccgg agtataatga
     1981 catactcaca aatacaaagg agaagctgag gcgggatccg aatgccagtg ccaggataga
     2041 ctttgagtat atgccagggg tgcctgacag gctcaggagt ttcctgcgga gaatggagag
     2101 cggaggactt ggattcgatg agtattagac cgcctgcaac agcggcaggg agcgtttcag
     2161 gcagaatccg gaggtctact gatactggcc tcctcccctg ctgccaaatt caatgacctc
     2221 catatcactg atatttgcgc catctatggt gaaccggagc atggtcataa ccttatgtat
     2281 gccggtacgg cctgctgcac cgggattaat acagagcatc ccgaagctcc tgtcatagat
     2341 cacttttgca atgtgagaat gcccgcagac aaagatatcc ggcttaagtt ccctgatcag
     2401 ctccagcgcc cgtggtgcat acctgccggg atagcctccg atgtgaatca tcagcacatt
     2461 ctttgagcct gcactgaaga agagatgttc tttcgttacc ctcctgacgt caaacccgtc
     2521 aatgttgccc catacggcac gcaacggcct gaacgcctca agttcggcga taacgccggc
     2581 attgccggca tcacctgcgt gccagatctc atcaacactg gcaaagtgct cataaacagc
     2641 aggatcaacc cagccgtgcg tgtctgatat gactcctatc ctcattcttc tgatatgaaa
     2701 attatgacaa cgctaaaatc tgtaaatttg cgtaaacaat tgacagaatg cagatttttt
     2761 attgtgaaga tatctccggc aaaaaagctc tcttcagcag ggaagaatca ggccactgcc
     2821 tcagggtgct gagaatgcgc cgtggtgaca ccctgacctt tactgacggc aagggaaatc
     2881 tttacaacgg agtgattgat acagatgacc caaaagggat gtctgtcacc atcacatcat
     2941 caaaaaatga ccccacgccc cgaaattaca ggcttaatgt ggcaatatca cccctgaaga
     3001 atgaggagag gctcgaatgg tttatcgaga aggcggtgga gaccgggatt gatgtcataa
     3061 cacctgttat ctgcagccgt tcggaaaaga agagtataag aaggaaaaga gcggaaggaa
     3121 ttatgatctc ggctatgaag caatcgggca ggtgcatcct gcccgggctt gaggaaccgg
     3181 aatcagcaga ggagtttatt cgcagacctg cgaaggagaa aaggcttatt gccacctgcg
     3241 actcttcatt tgacaggata gccataacgg aagcctttaa ccggggcgaa gatgttacga
     3301 tcctcatcgg tcctgagggc gatttcaccc cggcagaggt gcaggctgcc ataagcgccg
     3361 gttatgtgcc ggtacatatt ggcactggac gtctcagaac tgaaacagca ggcattgctg
     3421 cctgctgctc cgtttacctt gcaaatatct gaaatcaggt accagtcaag aaatcaaata
     3481 cagtggttat cttctttacc ggtacattgt aaaactctgt atagctgcat tttttacaga
     3541 ttatggctga gtatttcctg gactggatgt caaaaagctg tgtaagaaga ctgtgggcaa
     3601 ccctgatctc gcttagccgg taattcctgc agccgcattt cggacaggtg tactttatca
     3661 tagggatttc acttttcatc tttattctga tctgttttgt tcctcttgtc gcgtaatctg
     3721 ccggactttc gcagtgcctg atcaaggatc cattcaatct gaccattggt gctgcggaac
     3781 tcgtcggagg cccatttctc aatggcgctc agcttgtccc ggtcaatccg tattataaac
     3841 gacttcttct gcatgactaa tggtgcagcg taccggtgtt gatgatcgga ttgggatcct
     3901 tgtcgccgca caacaccacc ataagattac ttaccatggc tgccttcttc tcctcatcaa
     3961 gttctattat attcttgtca gagagctgct ccagcgccat ttcaaccatc gaaacagcac
     4021 cctctactat cttgaatctg gcggcaacca ctgcagcagc ctgctgacgg cggagcatcg
     4081 caccggcaat ctcctcggaa taggcaatgt aattgatcct tgcctcaatc acattgatac
     4141 ctgcaatcat cagccgctcc cttatctcct tctcaagctc atcattcacc tcctcaccgc
     4201 cactgcgaag cgtcatctca tgttcatgac cctcccagtt gtcgtaggga tagagaccag
     4261 ccagtttgcg aagagctgca tcgctctgca ccagaacgaa atgctcatac ttgtcaacgt
     4321 caaaggcagc cttgtaggtg tcttcaacct tccatacaag caccagacct atcttgatgg
     4381 ggtttccgtt cttgtcgttc accttgatgg gttcgctgtc aaagttgcgc gccctgagtg
     4441 aaatcctttt gcgtgaataa aacgggatat tccaccagaa accgtttttc ttcaccgttc
     4501 ccctgtaggc gccaaatagc accatcacca ttgattcatt cgggttaaca accatcagcc
     4561 ctgccaatag gaagatgtca accagtataa ggatgcagcc cagccagtat atctcgccaa
     4621 tgaaggaaaa aacggttgca gtaagtgcaa gaattgtaat taccaggaaa agatatcctg
     4681 atgacgctct gaaaattttt tcttctttca tgatttgtga tattattttg atatcacaaa
     4741 gatatcacta tatacaatac aagtcaagag aaacagaaaa aaaaattaca tttgagccat
     4801 gcatgagcgg catacagaca gggaacttta tttcagggag cagtcacaga ctacatcaaa
     4861 gtatgtaatt ccattcatct cgggtgtaaa gcaggtaacc tcctcacttt cggtgcttga
     4921 gataggttgt ggtgaaggtg gaaaccttct ccccttcctc agggccgggt gtgccagggt
     4981 gacggggata gatatttcgg aatacaagat tgggaatgcc cgcaggtttt attcatccat
     5041 ccccggggga gaaagtatgg agttcattgc tgcggatata tatgactgcg cagatgtggg
     5101 gaagtttgac cttgtaatga tgcgtgacgt gctggagcac attcatgacc atgcaaggtt
     5161 cattggcttt gtcaagaggc tgcttgcccg tgacagtgtt ctgtttatag ccttcccgcc
     5221 ctggaacaat ccctttggcg gacatcagca ggtatgtgac agccctgttc tgtcaaaatt
     5281 gccctggatt catctgctgc cacgcggaat ttacaggtgg cttctcaggg tggcaggaga
     5341 agagagcggg aagatagaag gtcttctgga gataagggat acaggaatca atactggcag
     5401 atttgagaag ataataagag aagagggatt ctccgttctg aagaaatccc tctggttcat
     5461 caatccgaat ttcgaagtaa agttcgggct tcgcccccgt ttactgccag gctttccggg
     5521 tgctatcccc ttcctgcgga atttcctggc aacaacatgt tactacatta tttcaatgcc
     5581 tcgtcaagag ccctgagcaa cggccgggcc gaaatctttg ttcctgttgc ctggctcatc
     5641 caatgctgtg gtgtgaggcg tcccagactg tacatgcggt caacctcatc agagaacttc
     5701 ctgcctttaa tctgttcttc tatctggaac tggattatct gtccgtaggc atagttagcc
     5761 aggtaaagag gggtactgac catatgtgaa tagatcgcga gcagtggcga atcgttcact
     5821 cccaataccg gggcaaagta gttgttccac acctccactg caatgtcaac ggtcttatca
     5881 ctcagctgtg caggcgttgc ctccgggttc tcataaagcc agttccacac tcccatctca
     5941 accaggctca cacccatgat ctccatgagt gaccacgctg catcaagcgt ctgaagcttc
     6001 tccttctccg gatttgcatc gttgattccc agcagcatca ggtcacggtc ctggaacaga
     6061 aacgcagtag cttctgtaaa tgcagtgttt ggaacacccg aaagggtata gtaatcaaca
     6121 tcataaagtg aaattgtctg ttccacgttg tgtccgaact catgtaccgc gatgttgtat
     6181 cccttataat ccattccctt gtccgatatc cgggttctca ggtgtgagac ggcccctttc
     6241 attgcggcgc cccatgcatg gccggagccg cgggccgggt caaccacgat cttgtctgca
     6301 agatacctgg ccctctcagg cgaccagccg agtttgacca gtatggcagg aaggccggcc
     6361 ctgaaggcag cagcatcagg ataaagggct gatgtctttg cggtgagcag gctttcagat
     6421 atcgagctcc ttgacttgaa cccgtcgtac caaatatcat atggtctgag gtcacgtcca
     6481 agcctctcct ttataatctc tgccacctgc ctgagctgag gtgagctgag atactccctg
     6541 aacagggctg cagcatcctc ctcggagatt tccatccctc cggaaaaact gcgaagcatg
     6601 gcggtattca taccgggatt atacgggtca gtcgctttca acgcattgaa tacgctgatg
     6661 atatgagaat agcgggtatc aggttcagca ggtgcatctg ccgcttcacc atccttcgtc
     6721 actttgttgg agtaaggggc ccactcatac ccgggattgt tgataacttt ttccggaatc
     6781 tcctgcctga ttatcctttc catcacccgg taaaccatct cctgcttttc gtttccggtt
     6841 tccctgtcgg catagttcga cttgatctcg tcgcgcaggt tccagtgaga gagaagcacc
     6901 atatcatcag gaaaaagctg ccgtccgtcg tcggtgcgaa gctttcccat atgaatattg
     6961 tattcagcaa tatacatatc ggcgttcccc agtgcagtgt tagcctgctg attcagttcc
     7021 gccggaacgc gtgacacaaa ctggtctccc agccgggcca tagcccactg ttcccggctc
     7081 cagccggcac caaggttatc cttctcctca agtgtgtaat acgggaagtt gagcgcaatc
     7141 acaaaggcaa tcttgttatc atagaaatct gactgaaggt gggaaccaac agaatagttt
     7201 ccgaacatgc ggtctatctc atcgatttca ccgccagtct cgtcaaggac tttcctcaga
     7261 tcgattgtaa cctcgttcat attcccggcc agtgactcaa gatagttgct gatcttgatg
     7321 aacacggact ttcgtttggc cgggtcagaa atatagttcg cggagacgaa atcgacaaat
     7381 tcctcagcgg ttccgtcctc ttcgcgccag agcgaggcgg catgttgcac gcccttttcg
     7441 acaagtaacc tgtcagcagc ggcctgttct cccattatac ggtcaatggc agaggtgact
     7501 gccgtttcac cgatgaaggc tgatgctgtc tctcctccct tttttgcagg ggtcgaacat
     7561 gctgtcaata ccatgaccat aagaaaaatt acttttttca tttctatctg tttatgatta
     7621 ttcttcaccg atgacctggg cagcgtagtg ccgggctgtc agcttctctc ccgttgcctt
     7681 ttcaatcatt tcattccact catatcgcat gccgggcata aatactttgg ttttaaagaa
     7741 ttcacccata gccttcatat cctcgccggt attgcccctg acaaccgagt ttgtgataaa
     7801 tgcagagaat tgtgaggcaa gaagttcacc aagaaggtag ttatggtaat agcacggata
     7861 gagagccaca tgtatcttgg tggcccagtc aggcatgtca cgccctgccg gtttcttcat
     7921 catctggtat ttttcaacca tatcccacca caattggtta aggtcctgat ccggattttc
     7981 atacatggct ttttcaaacc tgtacatcac ctgtgcccag cggctgaaaa caagttgttg
     8041 cagtctgaaa gcattgaagg cctcctcgtt tattctctct gcctcctttt catctatgac
     8101 acccatgaag tgcatccatg caggatcagt ggcatatctg ccaaagaaat tggccacagc
     8161 ctccgtggtg aatgtgtggg ctgcatccct gatggcaaag ggcagggacc tgtcgttgta
     8221 atacgaatat acgccatggc caagctcatg cattatggtg ttcatccagt atgaatcagg
     8281 cctgatattg tcaagagtgc ggacatctcc ctccctgtca atatctgtgg agaatgcatg
     8341 ctggttcttg cctggctttt cataaagatc actcctggca attatttcat caaccggcaa
     8401 tccgattgat gcgtaatagg ttgttgcaag cttaacggga tccttatcct tataataact
     8461 gtcaaaatca acagagtata tctctggtgc ctcctggaag taccggttct ggtaatgcca
     8521 tggcataagg tcagcggccg gaataccgta acgctttgaa taataatcat ctatctctcc
     8581 tttaagagtg gcgaaagtac cctctgtgag actgtcaagc tcatcaaaaa tccttgtaac
     8641 ctcctcagga tcctggcctg acagtttcag gctcatctgg tggtagttgc caaaacccag
     8701 ttcctgtgca atcctgttcc ggtgcctgac aagctttata acatcatctg ccacaaccgg
     8761 tccgatcttt ttatggcctt cccatgcagc ctgaagctcg ccggagttgg ttgagttgcg
     8821 aaggatatcc tccacctcat tgtcactcag catctttccg ttgacatcag ccctgaatga
     8881 gttgaatttc ttcgttatct ccgtctccat cctcacctgt tctgcaagaa ggcttgtgtc
     8941 aacctgacca gagaggaact ggttgtagat aacttccagt tgtcgtgcca gaaccgggtc
     9001 cttcaccttt cccgattcct tcaattcctt aagttcggcg aatgcctcct tatcagtgaa
     9061 gtagctgata taagccaggg aggccttttc gtttcttgcc catgcctcat cagtgccatt
     9121 gacattggca tcccatgaag agagggctga ttccttgtac aggggtatca cagctgcctc
     9181 gtaggaggca atgaaatccc gcattctgtt ctccatcttt tcccgttttg aggtgcaaga
     9241 ccataacatg gtcattactg caacgaaaca gacaatcagt cttttcatca taaaacaatt
     9301 ttggttttaa aggtattatg gaacccatat aactgatagt tacataaaca ggtgtttgtg
     9361 tatctgacat atccgggttt catgatgttt ataattgggt tgtgctaagg taaaaaaaac
     9421 atgcaagaat gaccatgacg ccagtcatat aatagaagaa ccggacaggt gattcctgcc
     9481 ggttccccga ccaatgttaa aagtatttga ggaaagtata tttagtttac cttattcttt
     9541 ctgatctttt cgaacagact ggcaatgact tcatctgacg gtgaatcttc catatcagga
     9601 agcagtacac tcagggcatt gtctatgccg aaagcatctg ctatcaggtc tgacggggta
     9661 ttggtaattt caaaatatga aagtaagagg gtagaattct tatccatagg cgtctctgtt
     9721 ctcatatcaa aaaaacgccc ccttatatca aaaattgtat cagccgattg aaagggtgat
     9781 atttttttcg accattaact tacgcatgtt gataagtgcg tatctcatcc gtcccagtgc
     9841 ggtgttaata cttactccgg tcatgtcagc aatctcctta aaactcattc cggcatagtg
     9901 acgcataata accacctcac gctgatcttc agggagaccg ttgataaggc gtcttacatc
     9961 acgcataacc tggcggcgca cgataatatc ctcaacattg tcatccgtca gcttcttcga
    10021 gttgaagaga tcagtctcat aatcatcatt gaagactata cccatctgct tctcatgcct
    10081 gaagtgatca ataataaggt tgtgagcaat cctcatcacc caggagataa acttgccgtc
    10141 gtcctggtaa cggcccgagc gtattgactg aactactttg aggaatgtgt cctggaaaat
    10201 atcatcggcc aggtccctgt tgcggatgta aaggcttata taggaataga ctttactttt
    10261 gtgtcggagg atgagcagct ctatgctttt ctcattgccg ttcatgaagc ttgtaataag
    10321 ctcataatcg gtcattttgc tcttcatatc taactctatt attggttttg ggtagagtta
    10381 ttctataggc gttcctcctc tgttaagttg ttattttttt ctgttaactg ttggcttata
    10441 cggtatggtg ttccgttttg tttcatttct gactcagaca aagaaaacaa aaaatagtaa
    10501 attcaaaatt gttaattttg ccaacttatc aaccagggct gaatgaaggg taatattgag
    10561 ataaagggag cgcgtgttca taacctcaag gatatctccc tctcaatacc acgaaacagc
    10621 cttgtggtca tcaccggcgt ctcaggatca ggcaagtcgt cactggcatt tgatacaata
    10681 tatgccgagg ggcagcgaag atatgttgaa agcctctcct cctatgcacg gcagttcctg
    10741 ggcaggatga acaagcctga tgttgatttc ataaacggta tccctcctgc cattgccatc
    10801 gaacaaaaag tcaacaccag gaatccaagg tctaccgtag ggacctccac tgagatattc
    10861 gactatctgc gtcttctctg ggcccgcgtg ggacggacaa tttctccggt atcaggaagg
    10921 gaggtgcggc gtcacaccgt tgatgacgtt gtcaattacc ttgcggccct gaccccggga
    10981 acaagggtgg tgcttactgc tcccattgca gtgccggata atgttgatcc ggctgaatac
    11041 atcaaattcc ttatccggca ggggtatacc cgtatcagca ccgaaaaggg tgtcatcaga
    11101 cttgacgatg caggaacgac agagctgatg acggaaatgc gcagcactga acttatcatc
    11161 gacaggtttg ctgccggtga ctcacctgag gaactgagcc gtgccgccga ttcggtacag
    11221 acggctttcc tgacgggaag aggtgaaata aaccttaccg tgaccgatga ggaaacaacc
    11281 cggacggtaa ggttctctga caggttcgaa gaagacggca tagccttcga ggagcccaca
    11341 gagcatctct tcagcttcaa ctctccgctg ggagcatgcc cgctctgcaa tggctacgga
    11401 aaaataatag ggatagacga gagcctcgtc attcctgacg aaaacctctc agtctatgac
    11461 aatgccatag cctgctggcg cggcgaaaca atgaagcggt ggaaggagag acttatctac
    11521 agtgctgaca aattcggctt tcccattcat aagccctggt acaaactgac ccctgagcag
    11581 aaggagctgg tatggaacgg caacaggtac ttcttcggac tgaaccgctt tttccagcac
    11641 ct
//
LOCUS       DAOPBK010000078        11342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5625_length_11342_cov_5.193497, whole genome shotgun sequence.
ACCESSION   DAOPBK010000078 DAOPBK010000000
VERSION     DAOPBK010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11342
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5625_length_11342_cov_5.193497"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLA17_06020"
     CDS             complement(<1..205)
                     /locus_tag="PLA17_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85006.1"
                     /translation="MLKRLNFRNLAAIALCLAVAAIFSGCDREDDMLFEIDGLTVDNY
                     PRVDGSSSTQPLNTLIACKLLGWR"
     gene            complement(608..1381)
                     /locus_tag="PLA17_06025"
     CDS             complement(608..1381)
                     /locus_tag="PLA17_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85007.1"
                     /translation="MKRYYNAKKAFLLALILQLLAFSCNEVITVHTRIVNIDSDDAFL
                     RLHFEFNKATYDPVSFVDRIDIFRNDSLICRLTCTSGKGIANWDFPSVPNGFQVNSSI
                     KGNEIPEILKSQNVRFEFGNITRYSGYGSWVYNPKYSDSSYRWMFDEEGNQNIKIDSF
                     YEPWVGRDIVKIESTKDFQVTDSGFELKNAKGEFVEFKVKYRRNPAYIIALVLDQSVK
                     TGEILYLKFRVDSTKVYNEMIIVPDKSIIGIAGKYRDLK"
     gene            1603..3495
                     /locus_tag="PLA17_06030"
     CDS             1603..3495
                     /locus_tag="PLA17_06030"
                     /inference="COORDINATES: protein motif:HMM:NF003780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HPK85008.1"
                     /translation="MKKIILVLFVMTVVPGCHQDLPAQKKNRKAGSAVQPAVTIKPEA
                     QKQVQTATVVPVIVDGEAQKIPAFENADDWIREDLWVETEFDSDGDGKRDRMHVDVTR
                     PKQTDTEGIKLPVVYESSPYFAGTAGNNKEYFWNVRHELNTVPKPHIHPPQIKRRGER
                     PVISNSQVKAWLPYGFAVVHSSAPGTGLSQGCPTIGTRIEALAPKAVIDWLNGRAKGY
                     TTPDGNEEVKAYWATGKVGMIGTSYNGTIPFAAATTGVEGLEAIIPVAPNTSYYHYYR
                     SNGLVRSPGGYLGEDADVLYDFIFSNPDNCNYCDSVVRDGEMAAGLDRVTGDYNEFWA
                     KRDLMNYMKPYKAATLMAHAFNDWNVVPSHSYRFIEKLKEMGVPLQIYYHQGGHGGEP
                     PFKMMNRWFTRYLFGIENGVENDPKAWIVREGAERLSPTAYTDYPHPDARPVTLYLTG
                     GAPEAGGLVMTKQAKQEKETLVDNYSFTGSSLAQAEYSNHRLLYLTPVLTEPVHISGV
                     VKVKIRAASSKPAVNLSVWMVALPWEDGRMTRMTESIITRGWADLQNHRSLTESEPLV
                     PGKFYEMSFGLEPDDQIIPAGKQIGLMIFSSDKEFTLHPVPGTELTVDLDGTEVTIPV
                     VGGVIK"
     gene            3981..5225
                     /locus_tag="PLA17_06035"
     CDS             3981..5225
                     /locus_tag="PLA17_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPK85009.1"
                     /translation="MKNSVLITLCLLVFAGLSSCSKDKGEEPDLTPKNIEVTAKSSEV
                     ITYSNEFGVDLFSKVALAENKNLMLSPLSASAALTMLLNGCGGDTYDQLKSTLKYPEQ
                     LTISEINEVYKSLVSQLLVVDPKVKLALANAIFYRMGFTVKPPFLSTMENDFNAEIDG
                     LDFSLPSALTTINKWASDNTNGKIPKVLEKISGDAVMFIMNALYFKGDWSYQFDKNLT
                     SDRVFYTDESNSVIVSTMNGDVGSKVTGGAGYNAIELPYGQTNFTMIVIVPTGTLADF
                     TASLDVVKWNTITAGFDSQEEYGELTVYMPKFKFSYEKQLNDQLQSMGMIDAFNPYLA
                     DLSGISDASIFVSFVKQNTFVEVDEKGTEAAAVTTIGINLTSMPSEPQVFKIDKPFVF
                     AIRERTTNTLLFIGQVVNPLDD"
     gene            5286..6587
                     /locus_tag="PLA17_06040"
     CDS             5286..6587
                     /locus_tag="PLA17_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85010.1"
                     /translation="MKNLIKSTLFLTVLIAGLIAGLTACKDRTTEMITYEANVPVYMP
                     FDEFRASFLKSAPAEISHPGKMYFKDGYLFVNEYGKGIHVIDNTDPSNPEKIAFYEIL
                     GNVDMAIKGNILFADSFIDLLAIDITDIENPVEIDRIENVFPEIVPEGDVWCPYAMVD
                     KSKGVIIDWKVQKITEEVAKYNNGGYIYYGEMSFMMNVDGAAKWTGISTTGSGTGGSM
                     ARFMLNEDYLYVIAVPTRLKTVDISTASEMTVVDSVDMPRMMETLFRLENNLFVGTTT
                     GMLIFDLVNPEKPVQISSYDHITACDPVVVDGQYAYVTLRSGNFCNNARNLLEVVDIS
                     SITNPYLVKSYPMFNPHGLGTDGNLLFVCDGAAGLKIYDKSNPLEIITSQVAHYPDFD
                     TYDVIPMDGILMLVGSGGIYQYDYSDPENIIQISHITIKEE"
     gene            6592..7329
                     /locus_tag="PLA17_06045"
     CDS             6592..7329
                     /locus_tag="PLA17_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85011.1"
                     /translation="MHRLTAIAVSLLLTLSATGQHRKSTVTLNDGSLISGTIISDSTD
                     YLYIKIIKPRIIKIGRGQVSSIEEATFPIKKNLKTSGYYVRLNTSFLFGKNESGNQGS
                     MSFQLSNGFQFRNGIAVGIGSGMEELGTALLPFYADFRFTPLNTGLSPYTWIKTGYSF
                     ALSDQDRSYYGESSTDKRTDGGFLFNAGVGISMFTWNRTAVNVGIGYRYQKVTLNQSY
                     YWWRGETVRHTITQYYRMEFHLGFVFM"
     gene            7408..7989
                     /locus_tag="PLA17_06050"
     CDS             7408..7989
                     /locus_tag="PLA17_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK85012.1"
                     /translation="MRNRIRDGHEYSDSQLVRGCADGDIRSQEILYRRYFSFAMSICI
                     RYTRDENEAMEIVNDSYMKVLDKISEYDPSMAFKNWYGKILVNTAIDNYRKNQKYSDY
                     LSISTITETEENEPEIEAELSANDILALYSYLPASCKVTFNLFEIEGYSHDEIGQMLG
                     VTASTSRANLAKAKKMLRELYYRQFSLARKSNE"
     gene            7982..9358
                     /locus_tag="PLA17_06055"
     CDS             7982..9358
                     /locus_tag="PLA17_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPK85013.1"
                     /translation="MSKFDDIFSRKAKEAFENYNADHLAGDGWNSFASRYGKQRRRTI
                     VFPLWARAASIIILVTAGVLFMNRFISRNPGEPTGLTAQQSGNEMSDSTEAQTDTNAV
                     ETATTASVPVIADNELSTRETVTGAGQTGNDGRRVRQATDTGGLDGLTPDTGNLASGQ
                     TGDAQTRKTGNLAAGQSNEPAIAVVTESDKMDDIYAGVNLALNPIEIRLTEKADSRLN
                     LPPVKVLDDYRAQPRQKMTTTLMTGLSGLMASIDNATTTAQGVSIGFYVERQLTRRIS
                     VRPGLAMAKHSYGMEGTPEEVLAGANTIMDYAAPELNGLSGTTSSYEADIDLLLMEVP
                     VNFVFSLWRRAEKNFFISTGASTMLYLSQHMTGSFNNTYTKTMADSYGGTTYATKTTT
                     VQVEREHHLLNRVDFLGLANFSAGYSMPFGRGTHLLLEPFVQLPVKDLTSMNLRIRYG
                     GLSMKIQF"
     gene            complement(9439..10854)
                     /locus_tag="PLA17_06060"
     CDS             complement(9439..10854)
                     /locus_tag="PLA17_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPK85014.1"
                     /translation="MTEKRDLTRALGLGYVIIIVISNIIGSGIFKKIAPMAAELHSGE
                     WILIVWLAAGLITLFGALSNAEIAGLLADTGGEFIYLRTIYGRFFSFLYGWSLFMVIQ
                     TAAISGIAYVFTQSLNSIIPIPDLLPSMQHLNIGGIFYPFQDFGIKLTTILLILFLTF
                     LNMSGLKSGAWVNKAILLIVGAGLLVIVIAGLTGGQEHAAGSMAVNQSPVRSTVTLSS
                     FYTAMLAAFWAYQGWVSVGFIGGEVKDPHRNIPKGIIWGVAIVIIVYLVVNYTYLRLL
                     SIPQLVEINNSGNQIAGVEAIRSLWGKGGVLFISLLILVSTLGCTNASILTGARPYYA
                     MARRGLFFRGAGRLNSHNVPSVSLLWQGIWASVLVLSGTFDQLTDMLIFAVYIFYGAT
                     VMGVFILRRRMPDAHRPYKTLGYPIVPAIFVLFCVGLFFNTIITRPREAGIGLSLIML
                     GIPVYFLLLRRGRNRDDSRDH"
BASE COUNT         3248 a         2617 c         2619 g         2858 t
ORIGIN      
        1 aacgccagcc caataattta cacgcgatca gtgtattcaa aggttgtgtt gaagaagaac
       61 catctactct gggatagttg tcaacggtta atccatctat ttcaaacaac atatcgtcct
      121 ccctgtcgca acccgaaaaa attgctgcta ctgcaaggca taaagctatt gcagccaggt
      181 ttctaaagtt taatctcttt aacatggtac aaagaacttg ttttacactg tctcaaatgc
      241 cttgcgatct ataacaatat tatcgaaata ttaacttttc ccccaagtcc tttttcaact
      301 atatcaaaaa gtcctcaaaa tagttcttgt aagcaattat ctcccggatt ttctccattt
      361 catcaagtta aacgcaaggg taactttaaa gctacaattt ttcaaattaa tcgatatccg
      421 tctttcttca tattatgccc cattacagaa tatgctaatc acataaatga atgcagacag
      481 caatatggcc tgtggcgggt tgcgggcgac ttctcattat ccactgttac aaaactttat
      541 gtggagcggt aagctttaaa gtgccactcc tttcgtcatt gtatatattg cgtgttgggc
      601 ttctttatta ttttaaatcc cggtatttcc ctgcaatgcc tattattgat ttatcaggta
      661 caataatcat ttcgttatat acttttgttg aatcaactcg aaatttcaga tataatatct
      721 caccagtctt tactgattgg tctaagacca aggctattat ataagcagga tttcttctgt
      781 attttacctt aaattcaaca aattctcctt ttgcattttt taattcgaaa ccggaatcgg
      841 ttacctgaaa atcttttgtc gattcaattt taacaatatc tcttcctacc catggctcat
      901 agaaagaatc aatttttatg ttttgattac cctcttcgtc aaacatccat ctatagctgg
      961 aatcagaata tttgggatta taaacccatg agccataacc agaatatctg gttatgtttc
     1021 caaattcaaa tcgaacattt tgactcttca gtatttccgg tatttcgttt cccttaattg
     1081 acgagtttac ctgaaaacca ttgggaactg aaggaaaatc ccagttagca attcctttgc
     1141 ctgaagtgca agtcaatcta catattaaag aatcattcct gaatatatca attctatcaa
     1201 caaatgatac cgggtcatat gttgctttat taaactcgaa atgaagtctt aaaaaagcat
     1261 catctgaatc aatatttaca attcttgtat gtactgtaat cacttcgtta cagctaaatg
     1321 ccaataattg aagaattagt gccaataaga atgctttttt tgcattgtag tatcttttca
     1381 taatgaaata caactttatt tagctcttgc aaaggtatat ttatttgacc gcagctttaa
     1441 tcagcctgcc aactttaagc agagccggaa taccagtgca gagtttaaac tgcagagcac
     1501 gatgaagccg gtcagctgcc tgttccaata atctgcccga taacatctct ttatcttcat
     1561 ttattttgtt cttttgaagg agtcattaat tgaacaaagt cgatgaaaaa gattatcctc
     1621 gtactctttg tgatgacagt tgttcccggc tgtcatcagg accttccggc acagaagaaa
     1681 aacaggaaag ctggatcagc agtccagccg gcagtgacta taaagcccga ggcacagaag
     1741 caggtgcaga cagctacagt agttccggta atagtagacg gtgaagcaca aaagatacct
     1801 gcatttgaaa atgctgatga ctggatccgc gaggatctgt gggttgaaac agagtttgac
     1861 tctgacggcg atggtaagcg tgacaggatg catgttgatg tcacccgccc gaagcagact
     1921 gacactgaag ggataaagct tccggtggtg tatgagtcaa gcccctactt cgcaggcacc
     1981 gccggcaaca ataaagaata tttctggaat gtcaggcatg agctcaatac cgtacccaaa
     2041 ccgcatattc atcccccgca gattaaacgg agaggcgagc ggcctgttat ctccaactcg
     2101 caggtcaagg catggctgcc ctacggtttt gccgtggttc attcatctgc acccggaaca
     2161 gggctctcgc agggatgccc cacaataggt acacgtatag aagccctggc acccaaggct
     2221 gtgatagact ggctcaacgg acgggccaaa ggatacacca cccctgacgg gaacgaggag
     2281 gtaaaggcat actgggccac cggcaaggtg ggaatgatag gtacatcata caacggaacc
     2341 atccccttcg ccgctgccac cacaggggtg gaaggactgg aggccatcat acctgtagca
     2401 cccaatacat catactatca ctattaccgt tcaaacggac tggtcaggag cccgggcgga
     2461 tacctgggtg aagatgccga tgtcctgtat gatttcatct tctccaatcc tgataactgc
     2521 aattactgtg acagtgtggt aagagacggt gagatggctg ccggcctcga tcgtgtcacc
     2581 ggtgactaca atgagttctg ggccaaacgt gacctgatga attacatgaa gccatataaa
     2641 gcagccacgc tgatggcaca tgccttcaac gactggaacg ttgtgcccag ccacagttac
     2701 cgtttcatcg aaaagctgaa ggagatgggg gtaccgctgc agatatacta tcaccaggga
     2761 ggtcatggcg gcgagcctcc gtttaagatg atgaacagat ggttcacccg ttaccttttc
     2821 gggattgaaa acggggttga aaatgatccg aaagcatgga tcgtccgcga aggagctgaa
     2881 cgactaagcc cgacagcata tactgattat ccccatccgg atgccaggcc tgttaccctg
     2941 tatctcaccg ggggtgcccc ggaggcaggc ggccttgtga tgacgaagca ggcaaaacag
     3001 gagaaggaaa cccttgtcga taattattcg ttcaccggct cttcccttgc ccaggctgaa
     3061 tacagcaacc atcgtctcct gtacctgact cccgttttga ctgagcccgt gcacatctcg
     3121 ggtgtggtta aggtaaagat cagggcagct tccagcaagc ctgcagtgaa cctctccgtg
     3181 tggatggtgg cattgccctg ggaagatggg cgcatgacga ggatgaccga gagcatcatc
     3241 acccggggct gggcagacct gcagaaccat cgctccctga ccgagagtga accgctggtg
     3301 cccggcaaat tctatgagat gagcttcggg ctcgagcccg atgaccagat catacctgcc
     3361 ggaaaacaaa taggcctcat gatcttctca agtgacaagg agtttaccct tcatcctgtt
     3421 cccggaacgg aactgactgt tgatcttgac ggtacggagg ttacaatccc ggtagttggc
     3481 ggggtaatca agtaatccgg acatcatcgg gtacccgtct ctgaattgcc cgaaagcaat
     3541 tcaccggttc gttatttaat ttaaagcaga gctgcgactg atatatcttc atctatatcg
     3601 tcccggtgaa tacctgtacc atcaccaata agccgccagc tacttaattg ttcatcagtt
     3661 gcccttctca gtcaggggaa ccaaagtaga ggtaccccaa cttctctgcc atcctgcagc
     3721 agcacataaa gcatatccct tgtgaaccac actttggtgg caagtgcttt tatagaatcg
     3781 taacctttta ttattaacac tgttgtcttt gctcaacaaa gatggcaaaa acaattgaga
     3841 cagcttttca ggcatcgggg agaacccaat ccgggtcact gcatgatttt tttctctgcc
     3901 atagcagcgt tttggcgttt catctcgtaa taggttataa aagacttaaa ttttgtttaa
     3961 ccattattaa aaagaatagt atgaaaaatt cagtattgat aactctgtgc ctcctggttt
     4021 ttgccggact atcatcatgt tcaaaggaca aaggagagga gcctgatctt accccgaaga
     4081 atattgaagt gacagccaag agctccgaag tcattaccta tagcaatgaa ttcggagtcg
     4141 acctcttttc aaaagttgct cttgccgaaa acaaaaacct catgctctca ccgctgagtg
     4201 caagcgccgc actgaccatg ctgctcaacg gctgtggggg agatacctat gaccagctga
     4261 agagcacgct gaaatatccc gaacagctga ccatcagtga aataaacgag gtatataaaa
     4321 gccttgtctc gcagttgctg gttgttgacc caaaggtaaa actggccctg gcaaatgcaa
     4381 ttttctacag gatgggattc accgtcaagc ccccgttcct cagcactatg gaaaatgatt
     4441 ttaatgctga aattgacggt cttgattttt ctctgccatc agcgctgaca acaattaata
     4501 aatgggccag cgacaataca aacggtaaaa tcccgaaggt gctcgaaaaa atatccggtg
     4561 atgcggtcat gtttatcatg aatgcccttt acttcaaggg cgactggagc taccagttcg
     4621 ataagaatct gacatctgac agagtgtttt acactgatga atcaaactct gtcattgtaa
     4681 gcaccatgaa cggggatgtc ggatcaaaag tgaccggcgg agcaggatac aatgcaatcg
     4741 aactacccta tggccagacc aactttacaa tgattgtcat cgtgccgaca ggcacactag
     4801 ccgacttcac ggcatcactt gacgtggtaa agtggaacac tatcacagca gggtttgaca
     4861 gccaggaaga gtatggcgaa ctgactgtct acatgccgaa gttcaagttt tcatacgaga
     4921 agcagctcaa tgaccagttg cagagcatgg gaatgattga tgcattcaat ccttatctgg
     4981 cagacctctc cggtatttca gatgcctcaa tatttgtcag tttcgtcaag cagaacacct
     5041 ttgtggaagt ggacgagaag ggaaccgaag ccgctgccgt caccacaata gggattaacc
     5101 tgacaagcat gccatcggag ccgcaggtat tcaaaattga taaaccattt gtctttgcca
     5161 tccgcgaacg gaccacaaac acccttcttt tcatcgggca ggttgtaaat ccgcttgacg
     5221 attaatccaa agaaccttta aagaaagaaa taactgaatt ttaaacatca ttaaatcaac
     5281 cagatatgaa aaacctgatt aaatccacac tgtttttaac agttctgatt gccggcctga
     5341 tcgctggtct gactgcctgc aaggacagaa caacagaaat gattacctat gaagccaatg
     5401 taccggtcta catgcctttt gatgaattcc gggcaagttt tctgaagagt gccccggcag
     5461 agatttccca tcccgggaaa atgtatttca aggatggcta tctgtttgtc aatgagtatg
     5521 ggaaaggcat ccacgtgatt gataacacag acccttcgaa tcctgaaaag attgctttct
     5581 atgagatact gggcaacgtt gacatggcta tcaaaggcaa tatcctcttt gctgacagct
     5641 ttattgacct cctcgcaatt gatataaccg atatcgaaaa tcctgtcgag attgaccgca
     5701 ttgaaaatgt tttcccggaa attgtccctg aaggagatgt atggtgcccg tacgcgatgg
     5761 ttgacaaatc gaagggagtg ataattgact ggaaagtgca gaagattacc gaagaagttg
     5821 caaaatacaa taacggcgga tatatctact atggagaaat gagttttatg atgaacgtcg
     5881 acggagctgc caaatggaca ggcatcagca ccaccggcag cggcaccggc ggatccatgg
     5941 cccgcttcat gctgaatgaa gactacctgt atgtcatagc agtaccaaca aggctgaaaa
     6001 cggttgatat cagcacggca tcagagatga cggttgttga cagtgtggat atgccccgta
     6061 tgatggaaac cctgttccgc cttgagaata accttttcgt gggaaccaca acaggaatgc
     6121 tgatctttga cctggtcaat cctgaaaaac ctgtccagat atcatcatat gaccatatta
     6181 cagcctgtga cccggtagtg gtcgacggcc agtatgcata cgtgacactg cgctccggaa
     6241 acttttgcaa caatgcaagg aacctgctgg aggtagtaga catatcctcc attacgaatc
     6301 cttacctggt gaaatcatac ccgatgttca atcctcacgg acttggtacc gatggaaacc
     6361 tgctcttcgt ctgcgacggt gcagcgggat tgaaaatcta cgataaatca aatccgctgg
     6421 aaatcattac cagccaggta gcacactatc ctgattttga cacctacgat gtaataccga
     6481 tggatggcat tcttatgctt gtgggttcgg gaggtatata tcagtatgac tatagtgacc
     6541 cggaaaacat aatccagatc agccacatta cgataaagga agaatagata tatgcaccga
     6601 cttactgcaa ttgcggtctc tcttctgctg acactatccg caacaggcca gcacaggaaa
     6661 tctactgtta cgcttaatga tggttccctt atctcgggaa ccatcattag cgattccact
     6721 gattacctct atattaagat tattaagcca cgtatcatca agattggcag agggcaggtt
     6781 tcatcgatcg aagaggcaac ctttccgata aaaaagaacc ttaaaacctc cggctattac
     6841 gtaaggctta acaccagctt cctgttcggg aaaaatgaaa gcgggaacca gggaagtatg
     6901 agctttcaac tttcaaacgg ttttcagttc aggaacggta ttgcagtagg gataggttca
     6961 ggaatggaag agttaggaac ggctctgtta cctttttatg cagatttcag gttcacaccg
     7021 ttgaatacgg gtttatctcc atatacctgg ataaaaacag gttatagctt cgccctgtca
     7081 gatcaggaca gatcatatta cggcgaatct tcaacagaca agcgtactga tggcggattt
     7141 ctcttcaatg caggagtggg catctccatg tttacatgga acagaactgc agtaaacgtt
     7201 ggcatcggat acaggtacca gaaggtcacc ttaaaccagt cttattactg gtggagaggc
     7261 gaaacagtca ggcataccat aacacaatat tacaggatgg agttccatct tggctttgtt
     7321 tttatgtagg aaaataaaaa tgataacagt aaccataata tcgtccgaat tgagttattt
     7381 cgggaaaact tgattcctga cggtagaatg agaaacagaa tccgtgacgg tcatgaatat
     7441 tcagactcac agcttgtcag gggatgcgcc gatggcgata tccgctcgca ggagattctc
     7501 taccggagat atttctcatt cgccatgtca atatgtatca ggtataccag agatgaaaat
     7561 gaagccatgg aaatagtaaa tgacagctat atgaaagtcc ttgacaaaat ctcggagtac
     7621 gatccttcca tggcgtttaa aaactggtac ggaaaaattc tggtgaatac ggccattgac
     7681 aattacagga aaaaccagaa atacagtgat tacctgtcaa ttagcacaat aacggagaca
     7741 gaggagaatg agccggagat agaggcagag ctttccgcta atgatattct cgctctctac
     7801 agctatctcc ctgcaagctg caaggtaacg ttcaacctgt tcgagataga aggatattca
     7861 catgatgaga tcggtcagat gctgggagtg acagcctcca catccagggc gaatcttgcc
     7921 aaggcgaaaa agatgctccg ggaactttat tacagacaat tttctttggc cagaaagagt
     7981 aatgagtaag tttgacgaca tattcagccg gaaggcgaag gaggcatttg aaaactataa
     8041 tgccgaccat ctcgcggggg atgggtggaa ctcttttgcc tccaggtacg ggaagcaacg
     8101 gaggcggacg attgtgttcc cgttatgggc cagggctgcg tcaataatta ttctggttac
     8161 tgcaggtgtt ctgttcatga accggtttat cagccggaat cccggagaac caaccggtct
     8221 gacagctcag cagagcggga atgaaatgag tgattcaaca gaggcccaaa ctgatacgaa
     8281 tgcggtagaa actgcaacca ccgcatcagt cccggtcatc gcagacaatg aactctctac
     8341 ccgggaaaca gttaccgggg ccgggcagac cgggaatgat ggcaggcggg ttcggcaggc
     8401 gacggatact ggtggcctgg acggtctgac tccggatact ggtaaccttg cttccgggca
     8461 aaccggagat gctcaaaccc ggaaaaccgg taacctggct gcaggacaaa gcaatgaacc
     8521 tgctattgct gttgtgactg aaagcgataa gatggacgat atttatgccg gggtaaatct
     8581 tgctctgaat ccgatagaga taaggttgac tgagaaagct gattccaggt tgaacctgcc
     8641 accagtaaaa gtccttgatg attatcgtgc tcagcctcgt cagaagatga ccaccaccct
     8701 aatgacaggc ttgtcagggt tgatggccag tattgacaat gcaacgacaa ctgcccaggg
     8761 agtttcaata ggcttctatg tggagcgcca gctgacacga agaatctctg ttcgtcccgg
     8821 actggccatg gcaaaacaca gttacggaat ggaaggcaca cctgaagaag ttctcgcagg
     8881 agcaaacacg atcatggatt atgctgcgcc cgaactgaat ggcctgtcag gcaccacttc
     8941 ctcatatgaa gcagatatcg atttgctttt aatggaggtg cctgtgaatt tcgttttctc
     9001 gctctggagg cgggcggaga aaaacttctt tatatctaca ggtgcgtcga ctatgctcta
     9061 tctgagccag cacatgaccg gaagcttcaa taacacctat acaaaaacca tggcagactc
     9121 atatggcggt accacatacg caacaaagac caccaccgta caggttgagc gcgagcatca
     9181 tctgcttaac cgggtcgact tcctggggct tgccaatttt tctgccggat actcaatgcc
     9241 attcggcagg ggaactcatc tgctccttga accttttgtg caattaccgg taaaggatct
     9301 caccagcatg aacctgagaa tcaggtacgg tggcctgtca atgaagattc agttctgaag
     9361 gattccctga cttgtgacaa ttagtgcggt agaaggttcc actttatcac taatggagca
     9421 aaatgacttt ccattagctc agtgatcccg ggaatcatcc ctgttcctgc ctcttctcag
     9481 cagaaggaaa tagacaggta ttccaagcat aataagggag aggcctattc ccgcttcgcg
     9541 gggcctagta atgatagtat tgaaaaagag tcccacgcag aaaaggacaa aaattgcagg
     9601 tacaattggg taccccaatg ttttataggg cctgtgggca tcgggcatgc ggcgcctgag
     9661 gatgaatact cccatgacgg tggctccgta gaagatgtag accgcgaaga tcagcatgtc
     9721 agtcagctgg tcaaatgtac ccgacagaac caggacagat gcccagatac cctgccacag
     9781 caatgataca gatggcacat tatggctgtt cagtcttccc gctccccgga agaaaagccc
     9841 ccgccgggcc atggcatagt agggacgcgc cccggtaagt atggaggcat tggtacaacc
     9901 cagggttgag acgagtatga gaagcgaaat gaacaacacc cctcctttgc cccacaggct
     9961 cctgatggct tccacgccgg ctatctggtt tccggaattg ttgatctcta caagctgagg
    10021 gatggagagc agccggaggt aggtatagtt aaccaccagg tagacgataa tgactatggc
    10081 tacaccccag atgattccct tgggtatgtt cctgtgggga tcctttacct cccctccgat
    10141 gaaccctacc gacacccatc cctggtacgc ccagaatgca gcaagcattg cagtgtagaa
    10201 cgatgaaagg gtcacggtgc tgcgaaccgg tgactgattc actgccatgc tccctgcggc
    10261 atgctcctga ccgccggtca gtccggcaat gacaatcacc aggagaccag cacctacaat
    10321 gagcaggatt gccttgttga cccatgcccc gctcttcagt cctgacatat tcaggaatgt
    10381 caggaacaga ataagcagga tggtggtcag ttttattccg aaatcctgaa aaggataaaa
    10441 tataccgcca atgttgaggt gctgcattga cggaagcagg tcagggatgg gtatgatact
    10501 gttgagtgac tgggtgaaga cgtatgctat gcctgatatt gcagctgtct ggatgaccat
    10561 gaagagtgac catccataca gaaacgaaaa gaagcggcca tagatagtgc gaagatatat
    10621 gaactcaccg ccggtgtcgg caagcaggcc ggcgatctca gcattgctca gcgccccgaa
    10681 aagggttatc aatcctgctg cgagccagac gatcaatatc cactcgcccg agtgcagctc
    10741 cgcagccatt ggggctatct ttttaaaaat acctgaaccg atgatattgg agataacaat
    10801 gattatcaca tatcccagtc caagagctct tgtaaggtct cgtttctctg tcataacaga
    10861 acacgctaat cagcgtgtat tgaagacaaa aatagaatta aaaatgaaaa tgttgttatg
    10921 cgaattatgc cggtaattaa tacgattatg gcagtagttg ccccaaaaat gaataagaga
    10981 ccagggctta tccgggtcgc tttattaaat aagcttaaaa gtttatgaaa tgaaatgcct
    11041 ctataagaat aaaaatagtt acttgtagac ggcaaacggt tgacggtatc cggcaaatct
    11101 gtaaccctca ccaattgcta attgctcatt gttaattgct aattgttcat tgctaattgt
    11161 tcattgctaa ttgccaacat ttatggaaag acgaaatttt ctcacagcac tcgggcttct
    11221 gacaggagga gtcctcacag gcctcagggg aagcagcctg ccatcacctg atgaagtcgg
    11281 ttacctcgac ctgcctccgg gcggtgatgt atggtcggtt atcaggcaac aatttttgtt
    11341 cc
//
LOCUS       DAOPBK010000079        11278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5656_length_11278_cov_5.439455, whole genome shotgun sequence.
ACCESSION   DAOPBK010000079 DAOPBK010000000
VERSION     DAOPBK010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5656_length_11278_cov_5.439455"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLA17_06065"
     CDS             complement(<1..440)
                     /locus_tag="PLA17_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HPK85015.1"
                     /translation="MKTTFGLLAISMIMFFAGCSGDRLSEATATVRGGIVQGTVEDGI
                     AVFRGIPFAAPPVGDLRWKAPRPVEPWDGVLTTDKFAPASIQPAVAWRGDEQTSEDCL
                     YLNVWTPAKSAKEKLPVMVWIYGGGFAFGGTAGAVTWGDQIAKHG"
     gene            complement(452..2122)
                     /locus_tag="PLA17_06070"
     CDS             complement(452..2122)
                     /locus_tag="PLA17_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPK85016.1"
                     /translation="MTLLIALLPDAADAQRLKPGPQDLSFFSSVDDTDQPYSVYIPEN
                     FDESKSYPLVVFLHGAWSNHRLGMRRLFGVGNSQGYDFIKPGNVPFENDVEATRYWPP
                     FRPVDYIAAAPLARGTAGYQGVAEQDVYDMIDDLRARFHIDEERVYLTGLSMGGGGTL
                     WLGLTRPDVWAAIAPVCPAPPEGTMELAGNASNLPVHLFIGDKDFLFQTAIEWKGKLG
                     ETALRLDYAEYPGVGHNSWEWAYKDGFIFDWFSQFKRDLYPEKVSFTTKWFKYNSAYW
                     VTIDDMIPGKTATAEAKFTGPNAVTVTVSGINAFTLRLEGHPQFNSSKKVSVNVNGKA
                     FSVRSADAVSFMLSGGNWINSRFTPGLTSKQKGAEGPLHAAVAGSHIYVYGTADNPPA
                     DVLAERRRQAMAAADWNGMGGRIMVFPRVVADRDVRPSDYVRSNLILFGTRETNSVIE
                     HFADKLPMHLDPKAENYGLVYIFPMNRHYILVNSGLPWWTPPKGQTTRTGYAFSGTIV
                     EILKMYQDFVLFRETPDDIITRGCFDNNWKLPADAAEALKADGAVKLN"
     gene            2343..2921
                     /locus_tag="PLA17_06075"
     CDS             2343..2921
                     /locus_tag="PLA17_06075"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HPK85017.1"
                     /translation="MNISETELVERVKKGDPQAFDMLYGIYAGRLYAFSMKYLKSQED
                     SEELVQAVFMKVWENQKKLDRDLSFKSFLFTIAYNEICRLFRRKSYLRKYVEESMYTA
                     SSSDNSVEESAGFHSVLEEVERIITRLPDKQRKAFIMCKIDGKPSKEVAAGLGLSPGT
                     IDNYVSSTVQLIRSKLSGESLAILLFASLFLI"
     gene            3048..4076
                     /locus_tag="PLA17_06080"
     CDS             3048..4076
                     /locus_tag="PLA17_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HPK85018.1"
                     /translation="MNKREREILTGYIDGTSGEAEQNEAISIMAAGAGNRNLKRMLEE
                     DWNVFAKGEGDPEVDLSPLLDTIHHQIRLKEYDEVRKPAIRFMALNTRIAAAIMIPLL
                     AGAGFLLFHDKTGNDGILSTASIYAPAGSRVSFLLPDGTEGMLNSGSTLAYSMPFTKN
                     RSVALTGEAWFEVAPDQKHPFTVNAGNLDLTVLGTAFNVSAYQEENYTEVVLERGKVL
                     VRCGHYKEGVVMNPSERLVYENGKVTRSSTDPSRYSSWTTGKLVFRGDVMSEVARRIE
                     RWYNVKVELMDKDLEKYSFRATFEDDSLEEVLKFLGMTSPIRYEITPRYLAEDSTYTR
                     SIVKIYLK"
     gene            4212..7640
                     /locus_tag="PLA17_06085"
     CDS             4212..7640
                     /locus_tag="PLA17_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPK85019.1"
                     /translation="MKKNNPGGWVLNRPLKKIFLTMRIAIGLLLLGIMQVYATDAYSQ
                     KTRVTLNMTDTRLVTVLDKIEEETEFFFLYNEKLLDTDRIVSVSVDNQLIEVVLDKLF
                     AGTDVTHTIIDRKIILAPGYLAETTAELQQRVTGKVTNPNGEPIPGVTVTVKGKILGT
                     ATAMDGSFSLDNVQPTDILIFSFIGMKTEEILVGSQTLFNIVLSEELIGLDAVVVTAL
                     GIKREEKALGYAVQKVSGDVVSTVKGVDMGTSLTGKVAGMLIKNSSEFSAEPEILVRG
                     EKPLLVIDGVPYGNMSLRDLPSDDIENISVLKGATATALYGSRGSGGAIMVTTRRGAD
                     KNGLTVTLNSSTMFTAGYLAIPETQSTYGRVVNTSTNTYARSGDGSWGVPLDGREIIQ
                     WDPVSKSLKLLPYLPAGKDNFKNFLEQGFVLNNNISIVQQSDLGSLRASATTVQNKGQ
                     YPNSMFNKMTYTIGGDMKINRFSLSSSIAYNKQNSPNVGFSGYTGYDPMYNILVWSAP
                     DYDIRDYKDYWVVPDEVQNSSYTSTNNNPYFDRYERIRSVNKDVFNVSLDMGYDIMPW
                     LKAIVRAGYDIYSDRQEVRISKGSFQGGGSSTVIPGGSQIWGESQRGSYNLGLSRGYS
                     INNDLMLTADKSFNNFTVSALAGGTIFFRQDDGIEARTQGGLSIPGFYSLKASINPAV
                     VGSTLSRQQVNSLYGRLALSWKSLVYAEATLRNDWSSTLSKTTRSYLYPSVSGSFIAS
                     NLLPEIEWLSLWKIRSSWTSYKKPANVFDINSVYSITNNAWGSLSAASLPTTIRGADL
                     LPESAETFEIGTMVNIFNNRASVDVTWYRKRMYDFIKYAPITPASGFSNRLLNIDEEW
                     ARKGLEIEANVTPVKSENWQWDMGFNWSTYATYYLQLDSLHSADKPWVQVGARTDHYV
                     LRDYQKDPEGNIIHNNGLPLYSGYDSKFGNYDPDWIWGLSNTVRYRNWTLTVSADGRV
                     GGIAQTTTEMYMWRAGSHPGSLAPERYLDATQPGTKNYIGAGVKVVEGSVTYDTYGNI
                     TSDTRVYAPNDVAVTYKTYTEALHKGTAWGGAPSTIDAYNTTFFKIREVALTYRLPQE
                     LCSRLGINGVSVSAIGQNVFLWAKQFKYSDPDGGSENFIDPAVRYLGLNLKIGF"
     gene            7669..9186
                     /locus_tag="PLA17_06090"
     CDS             7669..9186
                     /locus_tag="PLA17_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPK85020.1"
                     /translation="MKKIMLLVMAMALIFAGCNKFEEYNTDPNKTTKASASMLCTRAI
                     LNVTSFSGRDGKVMISDNALPKYVGYANEGQLGTQYNTIGATDFGGLLILPNIEQMLE
                     EAKGTIMENSYKGVASFVKAYRFFRLTMETGDMPYSQANKGAEGLYRPEYDTQEQIFL
                     GILDDLKAADQYFAQGVAFNGDPTPFNGDPDKWRRAVNATALKILMTLSKKENTSMLN
                     VKSRFAEIVNAGYLMQPSTGFLGVNYSTQNKHPLSGTNDLFTSRTVMSSLLVDNLKLL
                     NDRRMFYYAEPAAAQIIAGFTQDDPEAYTGVDVSIDYATMNAGHSAGIYSLLNKRYLQ
                     EEATEPRRLMTYAEQQLILAEARILGWITTGTAQEYYEQGVKAALGHFLTVNSNYAHT
                     MPINQAYIDGYFAGEAAFKADPADQLKQIWMQRYLLNFMQDAQYSYFEYRRTAYPEFP
                     INPATSLNENNPDGLPMRWLYPSSETNYNRENLIEALNRQYEGYDEINKLMWLLK"
     gene            complement(9275..9706)
                     /gene="fucU"
                     /locus_tag="PLA17_06095"
     CDS             complement(9275..9706)
                     /gene="fucU"
                     /locus_tag="PLA17_06095"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HPK85021.1"
                     /translation="MLKGISPLISPDLLNVMARMGHGDELVLADAHFPGESIAKRVVR
                     ADGLRIADLLEAIMPLFELDSYVPHPLIMMEPVPGDSLDPGVEISYLERIMKEVPVVP
                     PVKRIDRFAFYERAAKAFAVVMTGEIAKYGNIILIKGVTPM"
     gene            9872..11080
                     /locus_tag="PLA17_06100"
     CDS             9872..11080
                     /locus_tag="PLA17_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK85022.1"
                     /translation="MNNNRREFLKKGLLGVSAAALIPGVTKGSPVTAAATASQADLPA
                     RVLGRTGIKAPLISLGAGGTITPGFVKGAYNAGIKLFFSATYYGEGNNEIIVGEALKD
                     LPRDSFVVATAAPCNEHDNRTGLLSDKFNARAYLEKAEGSLRRFGLEQIDIILFPFAS
                     KKETVCNEEILRTMEQLKESGKVRFTGIASHGGIEETLIAAASTGIYDVAMISYNFKS
                     QNKEALDDAIAKAVASGMGIVAMKTTAGAFRNKSGPGINSDAALKWVLQNANISTIVS
                     GMTTVEQMQKNLAMIKNLKMTEEEIADLENALAFSETGLYCQQCGECLPQCPARLDIP
                     TIMRGYMYAYGYRNLLQARNTLEASGLPSVPCADCESCSVKCSSGFDVRGKIQDIARL
                     TKVPEEFLLT"
BASE COUNT         3110 a         2801 c         2726 g         2641 t
ORIGIN      
        1 ccgtgctttg caatctggtc tccccaggtg actgcaccgg ccgttcctcc gaaggcaaaa
       61 cccccgccat agatccatac cattaccggc agtttctctt ttgccgactt tgcgggagtc
      121 cagacattca ggtaaagaca atcttccgaa gtctgctcat ctcccctcca cgcaacggca
      181 ggctgaatgc ttgccggtgc aaatttgtct gttgtcagca caccatccca tggctcaacg
      241 ggccggggtg ccttccagcg taagtctcca acgggcggtg cggcaaaagg aatgcccctg
      301 aaaacggcaa tgccatcctc aacggttccc tgcacaatac ctcccctgac tgttgcggtg
      361 gcctctgaaa gccggtctcc gctgcatcct gcaaaaaaca taatcattga tattgccaat
      421 agtccgaaag tggttttcat tgtatttggt tttagttaag tttcacagcc ccgtcggcct
      481 tcagcgcctc tgccgcatca gccggaagtt tccagttatt gtcaaaacag ccccgggtaa
      541 ttatatcgtc tggcgtctcc ctgaagagaa caaaatcctg gtacatttta agaatctcaa
      601 cgattgtccc gctgaaggca tatcctgtcc gggttgtctg ccccttcggt ggcgtccacc
      661 agggcaagcc cgaattgacg agtatgtaat gcctgttcat ggggaagata tagacaagtc
      721 cataattctc agccttcgga tcaagatgca tcgggagctt gtctgcaaag tgttcgataa
      781 ctgaatttgt ttcgcgtgtt ccgaaaagga tcagatttga cctgacgtag tcacttggcc
      841 gtacatccct gtcggccacc accctgggaa ataccattat cctgcctccc ataccgttcc
      901 agtcggcagc ggccatggcc tgcctgcgcc tttcggccag cacgtcagcc ggaggattgt
      961 cagccgtgcc gtaaacatag atgtggcttc cggccactgc agcatgcaga ggcccctcgg
     1021 cacccttctg ctttgaggtt agccccggcg taaacctgga gtttatccag ttccctcccg
     1081 aaagcatgaa tgagactgca tcggcgctcc tgacgctgaa agctttcccg tttacgttta
     1141 ccgacacctt ttttgaggag ttgaattgcg ggtgaccttc aagacgaagg gtaaaggcat
     1201 ttattcccga cacggtaact gtaacggcgt taggtccggt aaacttagcc tctgccgtcg
     1261 cagtttttcc gggtatcatg tcatcaatcg taacccagta ggcgctgttg tatttgaacc
     1321 attttgtggt gaatgacact ttttcgggat acagatccct tttaaactgc gagaaccagt
     1381 caaatatgaa gccatcctta tatgcccact cccagctgtt atgccccaca ccagggtact
     1441 ccgcgtaatc aagcctcagg gctgtttccc cgagcttgcc cttccattca atagcggtct
     1501 ggaagagaaa atccttgtcg cctataaaga gatgcaccgg caggttggat gcattgccgg
     1561 caagctccat ggttccctct ggcggtgccg gacagacggg agctattgcc gcccatacgt
     1621 ccggccgggt taggccaagc cagagagtgc cgccgccacc catagagagg cctgtgagat
     1681 ataccctctc ctcgtcaata tgaaaccgtg ccctgaggtc atcaatcatg tcatagacat
     1741 cctgctccgc cacgccctgg tagccggcgg tgccacgtgc cagcggcgca gctgctatgt
     1801 aatccacagg cctgaaagga ggccagtagc gtgtggcttc cacgtcgttc tcaaacggga
     1861 cgttgcccgg cttgatgaaa tcatatccct ggctgttgcc cacgccgaaa agacgtctca
     1921 tcccaagcct gtgattagac caggctccat gcagaaacac cacaagcgga taggatttcg
     1981 attcatcaaa attctccggg atatagaccg agtatggctg gtcagtgtca tctaccgatg
     2041 agaaaaaaga tagatcctga ggcccgggtt tcaggcgctg ggcatcagca gcatcgggca
     2101 gaagcgctat taaaagtgtc accagaatta tgctccggat taaaaagata attcttctca
     2161 ttatgcgttc aattcagttt ttgtttaaca tcatgtgacc ataaaagtag ttttttaaag
     2221 tggatttcgt gacactatca ccccaataaa gtcagtatat gccggttcat aagattcaga
     2281 tcagaggagc accgttttta tgagttatct gattatattt gatgcaatgc aatttcctgt
     2341 atgtgaatat atcggagact gaactggttg aaagagttaa aaagggagat ccgcaggcat
     2401 ttgacatgct ctacggcatc tatgccggcc gtttgtatgc cttcagcatg aagtacctca
     2461 aatcacagga agattccgag gaacttgttc aggctgtctt catgaaagta tgggaaaatc
     2521 agaaaaagct ggacagggac ctctcgttca aatcattcct ttttaccatt gcctataatg
     2581 agatctgcag gctctttcgc cggaaaagct atctcaggaa atatgttgaa gaatcaatgt
     2641 acacagcaag cagttctgac aattcggtgg aagagagtgc aggttttcat tcggtgctgg
     2701 aggaagtgga aagaataatc acccggcttc cggataaaca gagaaaggct ttcataatgt
     2761 gcaagattga cggtaaaccc tcgaaagagg tggcggctgg tcttggtctt tcaccgggca
     2821 ccattgacaa ctatgtctcg tcaacagtgc agttgatcag gtcgaagctg tcaggagaga
     2881 gccttgctat cctgcttttc gcttcccttt ttcttatctg actgtctccg ggaggtctgt
     2941 cagaaatatt ttcatctttt ttatatgaga atttagcccg tcacgggtat tactgttgtg
     3001 gaaagggtaa aaactccggg taacacaaaa aacttctgat gacattgatg aataaaagag
     3061 agcgggagat acttacagga tatattgacg gcacctccgg agaggctgaa caaaatgagg
     3121 caatttccat catggctgcc ggagcaggca acaggaacct gaaaaggatg cttgaagagg
     3181 actggaatgt ttttgctaag ggcgaaggtg accctgaagt ggatttatca cctctccttg
     3241 acactataca ccaccagatc agacttaaag agtatgacga agtcaggaaa cccgcaataa
     3301 gattcatggc actcaataca agaattgccg cggccattat gattccgttg cttgcagggg
     3361 caggcttcct gcttttccat gacaagaccg gcaacgatgg tattctgtca actgcctcca
     3421 tctatgcgcc tgccggctcg cgggtatcat ttctgcttcc tgatggtacg gaagggatgc
     3481 tcaacagcgg ttcaaccctt gcatattcaa tgccctttac aaaaaaccgg tccgttgccc
     3541 tgacaggtga agcatggttc gaagtggcgc ctgaccagaa acatcccttt actgttaatg
     3601 caggaaacct tgacctcact gttctgggca cagcttttaa cgtaagcgcc taccaggaag
     3661 aaaattatac cgaggtggtg cttgaaagag ggaaagtgct ggtcagatgc gggcattata
     3721 aggaaggagt ggtaatgaat ccttcagaaa gacttgttta tgaaaacggc aaggtgacaa
     3781 ggagctcaac cgatccttcc aggtattctt cgtggaccac cggaaagctg gtcttcaggg
     3841 gtgatgtcat gtcagaagtg gcaaggagaa tagaaagatg gtataatgta aaggttgagc
     3901 ttatggacaa agatcttgag aagtactcct tccgcgctac ttttgaggat gactcactcg
     3961 aagaggttct taaattcctg ggaatgacct cgcctataag gtatgaaata acaccccggt
     4021 atctggcaga agattcaaca tataccagga gcattgtaaa aatctatctt aaataataac
     4081 cctttaacca aatcactgag cccatgaaaa aaacagagag cccttaacct gaaaaaaaag
     4141 gaaatcgcgg caacgatttc cttaaaaaac atccttgaat gatatttttt aacctgaaaa
     4201 ctttcaaagt tatgaaaaaa aacaaccccg gggggtgggt gcttaaccgg cccctgaaaa
     4261 aaatctttct aacaatgcgc atagcaatcg gacttctgct gctcggtatc atgcaggtgt
     4321 atgcaactga cgcatattcc cagaagacaa gagtgacgct gaacatgacc gataccaggc
     4381 ttgtgacagt actcgacaag atcgaggagg agacggaatt cttcttcctc tataatgaaa
     4441 agcttcttga cactgaccgg atagtttctg tttcagtaga taatcaactg attgaagtag
     4501 ttctggacaa attgttcgcc ggtactgacg ttacccatac gatcatagac cgcaagataa
     4561 tccttgcccc tggttacctg gccgaaacaa ctgctgaact acagcagaga gtaacaggaa
     4621 aggtgacaaa cccgaacggc gaaccgattc ccggtgttac agtgaccgtt aagggaaaga
     4681 tactcggaac ggcaaccgcc atggacggca gtttctcact tgacaatgtc cagccaactg
     4741 acatcctgat cttctcattc atcggtatga aaaccgaaga gatcctggtc ggatcacaaa
     4801 ctctgttcaa catagttctt agcgaagaac tcatagggtt ggatgcggtc gtcgtaactg
     4861 ccctcggtat aaaaagggaa gaaaaggcgc tgggctatgc agttcagaaa gtctccggcg
     4921 atgtagtctc aaccgtcaaa ggtgttgaca tgggcacttc gctgaccgga aaagtggcag
     4981 gcatgctgat caaaaacagt tctgaattca gcgctgaacc tgaaatactt gtccgtggag
     5041 aaaaaccgtt gctcgttatt gatggggtcc catacggcaa tatgtccctc cgtgacctgc
     5101 cctctgatga tattgaaaat ataagtgtgc tcaagggagc taccgctact gcactttatg
     5161 gatcaagagg ctccggcgga gcaattatgg ttacaaccag acggggtgcc gacaaaaacg
     5221 gactcactgt tactcttaac agtagtacta tgtttactgc cggatacctg gcaatcccgg
     5281 agactcagtc aacctatggc cgcgtagtaa acacctctac aaacacttat gcccgctccg
     5341 gcgacggatc atggggtgtg ccccttgatg gcagggaaat cattcaatgg gatccggttt
     5401 cgaaatcatt gaagcttctg ccctacctgc cagccggcaa ggacaacttc aagaacttcc
     5461 tcgaacaggg cttcgtgctg aacaacaaca taagcattgt acagcagagt gacctcggca
     5521 gcctcagggc atcggcaaca accgtgcaga acaaaggaca gtatccgaac tccatgttca
     5581 acaagatgac atacaccatt ggcggtgata tgaaaattaa cagattctcg ctttcttcct
     5641 caatcgcata caacaagcaa aactccccta acgttggctt cagcggatat accggatatg
     5701 acccgatgta caacatcctg gtatggtctg ctcctgacta tgacataagg gattacaaag
     5761 attactgggt agtgcctgat gaggtgcaga acagcagtta taccagcacc aacaacaacc
     5821 cttatttcga caggtacgag aggattcgca gtgtcaacaa ggatgtcttc aatgtcagcc
     5881 ttgacatggg atatgacatc atgccatggc ttaaggcaat cgtccgagcc ggatatgaca
     5941 tctacagtga ccgtcaggag gtaaggatct ccaagggctc tttccagggc ggaggaagct
     6001 ctactgtcat cccgggagga agccagatat ggggtgaatc ccagagagga tcatacaacc
     6061 tcggcctcag caggggctat agtataaaca atgacctgat gctgacagct gacaaatctt
     6121 tcaataactt tactgtcagc gccctggcag gtggcacaat cttcttccgt caggatgacg
     6181 ggattgaggc aaggacacag ggtggcctct ctatccccgg attctattcc ctcaaggcct
     6241 ccatcaaccc tgcagtggtg ggatcaactc tcagccgcca gcaggtaaac agcctctacg
     6301 gccgcctggc tctctcatgg aaaagccttg tttacgcaga ggcaaccctg agaaacgact
     6361 ggagctcaac cttatcgaaa acaacaaggt cctatctcta tccctctgtt tcaggaagct
     6421 tcattgcatc aaacctgttg ccggagatag agtggctctc actctggaaa atccgcagct
     6481 catggacctc ctacaagaag ccggccaatg tgtttgatat caattcagtc tattccatca
     6541 ccaacaatgc gtggggttct ctcagcgcgg catccctgcc aacaactatc aggggagctg
     6601 accttcttcc cgaatcagca gaaaccttcg agatcggaac aatggttaac atattcaata
     6661 acagggcctc ggttgatgtc acctggtaca ggaagagaat gtatgacttt ataaaatatg
     6721 cacccataac acccgcctca ggattctcca acaggctgct gaacatagat gaggagtggg
     6781 ccagaaaagg ccttgagatt gaagcaaacg tgaccccggt caaatcagaa aactggcagt
     6841 gggacatggg tttcaactgg tcaacctatg caacatatta cctgcagctt gactctctcc
     6901 actccgctga caagccatgg gtacaggtag gcgccagaac agaccactat gtgctgagag
     6961 actatcagaa agatcctgaa ggaaacatta ttcataataa cggccttccc ctctactcag
     7021 ggtacgattc caaatttggc aactatgatc ctgactggat atggggcctg agcaacaccg
     7081 taaggtaccg gaactggaca cttacggtct ctgccgacgg aagggttggt ggtatagcac
     7141 aaaccactac cgagatgtac atgtggagag ccggctcaca tcccggttca ttggcccctg
     7201 aacgctacct tgatgccaca cagcccggca caaaaaatta tatcggtgca ggtgtcaaag
     7261 ttgtagaagg atctgtcaca tatgacacat acggtaatat tacaagtgac accagggtgt
     7321 atgcccccaa tgatgttgca gttacttata aaacctacac cgaagccctg cataagggta
     7381 cagcctgggg aggagcacct tcaaccatag atgcatataa caccaccttc ttcaagataa
     7441 gggaagtggc cctcacttac cgtctgccac aggaactctg tagcaggctg ggcattaacg
     7501 gagtttccgt ctctgccatc ggccagaacg tgttcctttg ggcaaaacag ttcaagtatt
     7561 cagaccctga cggaggctca gaaaacttta ttgacccggc agtgagatac ctggggttga
     7621 acctgaagat tggtttctaa tcatttaaaa agcagaatga tgaaaaagat gaaaaagata
     7681 atgctcctcg tgatggcaat ggccctcatt ttcgccggtt gcaacaaatt cgaggaatac
     7741 aatactgatc ctaacaaaac tacaaaagcg agtgcatcca tgctatgcac ccgggcaata
     7801 ctgaatgtaa cctccttcag cggccgcgac ggcaaggtca tgatatcaga caatgccctg
     7861 cccaaatatg tcggctatgc caatgaagga cagctgggaa cacagtataa cacaattggt
     7921 gcaactgact tcggcggact gctgattctt cccaatattg aacagatgct tgaagaggct
     7981 aaaggcacaa ttatggaaaa ctcatacaag ggtgttgcaa gctttgtaaa agcctacagg
     8041 ttcttcaggc tgacaatgga aaccggtgac atgccctact ctcaggccaa taaaggagct
     8101 gaagggctgt acaggccgga atatgatacc caggagcaga tattccttgg aatcctcgac
     8161 gacctgaaag ctgccgacca gtactttgcc cagggggtag ccttcaacgg cgatcctaca
     8221 cctttcaatg gtgacccgga taagtggcgc agggctgtga atgcaaccgc actcaagata
     8281 ctgatgaccc tgagcaagaa ggagaatacc agcatgctca atgtaaaatc acgcttcgcc
     8341 gagatcgtta atgcaggtta tctgatgcaa ccttccacag gttttctggg cgtgaattat
     8401 tcaacccaga acaagcaccc gttatccggc accaatgacc tctttaccag cagaactgtc
     8461 atgagctcac tgctggttga caatctcaaa ctcctgaatg acaggcgtat gttttattat
     8521 gcagaacctg cggcagccca gataattgcc ggctttaccc aggatgaccc ggaagcctac
     8581 accggtgttg atgtttcaat tgactatgcc acaatgaatg ccggccacag cgccggaatc
     8641 tattccctgc tgaacaaacg ctatcttcag gaggaagcca ctgagccgcg caggcttatg
     8701 acctacgcag aacaacagct gatcctcgct gaggccagaa tcctcggatg gatcaccaca
     8761 ggtacggcac aggagtacta tgaacaggga gtcaaagctg ctctgggaca tttccttacg
     8821 gttaacagta actatgcaca cacaatgccg atcaaccagg cgtacattga cggttacttc
     8881 gccggcgaag ctgctttcaa ggctgatcct gcagatcagc tgaagcagat ctggatgcag
     8941 cgttacctcc tcaattttat gcaggatgca cagtacagct actttgagta caggagaacc
     9001 gcttatcccg aattcccgat taatccggca acaagcctta acgagaacaa cccggacggt
     9061 ctcccgatga ggtggctgta tccttcatcg gaaaccaatt acaacaggga aaacctcatt
     9121 gaagccctta acaggcagta tgagggttat gatgagatca acaagcttat gtggttactt
     9181 aaataggttg gttagtgatt tgagggatac cttgccggtg ttgagttccg gttatcaggt
     9241 aaaagccccg ggtttttgtt ccggggcttt ttttctacat gggtgttaca cccttgataa
     9301 gaataatatt cccgtatttg gcaatctctc cggtcattac cacggcaaaa gctttggctg
     9361 cacgttcata gaaggcgaac cggtcaatcc ttttcacggg cggaacaacc ggtacttctt
     9421 tcatgatccg ttccaggtat gaaatctcaa caccgggatc aagtgaatcg cccggaacag
     9481 gttccatcat gataagcggg tgtggcacat aactgtcaag ctcgaaaagc ggcatgatgg
     9541 cctcaagaag atcggcaatg cggaggccgt cggccctgac aaccctttta gcgatacttt
     9601 caccagggaa gtgggcatca gccagaacca gctcgtcacc gtgacccatc cgggccatca
     9661 cattaagcag atcgggagaa atgaggggag aaataccttt aagcataata cctgtttttt
     9721 gttccgtgaa tttacaattt tccgaggagc gggggaaatt gccagacagt aaatgcatct
     9781 gatattcagt ttttgataaa ttagcaaaca ggagatggat atttttaaat acaagagact
     9841 gaattacctt ctgtaacaaa aaaactgcat catgaacaac aaccgtcgtg aatttctgaa
     9901 gaaaggctta ctgggtgttt ccgcagcagc attgatcccc ggtgtgacaa aaggctcccc
     9961 cgttacagct gcagcaacag cctcccaggc tgacctgccg gcaagagtgc tgggccgtac
    10021 gggaataaag gcaccgctta tcagtcttgg tgcaggtggc acaattactc ccggctttgt
    10081 caaaggggca tacaatgcag gtattaaact attcttctca gccacctatt atggtgaggg
    10141 gaataacgaa atcatcgtcg gcgaagcact taaggatctc cccagagact ccttcgttgt
    10201 ggccactgca gctccatgca acgagcacga taaccgcaca ggcctgctaa gtgacaagtt
    10261 caatgccagg gcatacctgg agaaggcgga aggcagcctc aggcgtttcg ggcttgaaca
    10321 gattgatatc atactctttc cctttgcttc caaaaaggag actgtgtgca acgaggagat
    10381 tctcaggaca atggaacagc tcaaagagtc gggtaaagtc agatttaccg gcatagcctc
    10441 acacggcggc attgaggaaa cactcatcgc agctgcctca accggcatct atgatgtcgc
    10501 gatgatctca tacaacttca agtcacagaa caaggaggct cttgacgacg ccatcgcaaa
    10561 ggctgtagct tcagggatgg gcattgtcgc aatgaagact accgccggtg cattccggaa
    10621 caagagcgga cccggaatca attctgacgc agcgttgaaa tgggtcttgc aaaatgccaa
    10681 catatcgaca atcgtttccg gcatgacaac agttgaacag atgcagaaga acctggcaat
    10741 gatcaaaaac ctgaagatga ctgaagagga gattgcagat cttgaaaacg ctcttgcatt
    10801 ttcagaaaca ggactctact gccagcagtg cggagagtgt ctgccccagt gccccgccag
    10861 gcttgacata ccaacgataa tgcggggtta tatgtatgca tacggataca ggaacctgct
    10921 gcaggcaagg aacaccctgg aggcatcagg gctgccgtca gtgccctgcg ctgactgtga
    10981 atcatgcagc gtaaagtgca gttcgggatt tgacgtcaga gggaagatac aggacatagc
    11041 gcgcctgaca aaagtgcctg aagagttttt actgacctga taccagggaa agaagccaat
    11101 aaccagagag accccggggg ctatgttcag aagaaatccc agtatggtca ggatctccag
    11161 tacaaaaaaa gagcaggaac tacgctcctg ctttcgcatt tgcatcaact cccgcactta
    11221 ctggtagccc caccaggaat cgaacctgga tttcaggttt aggaaacccg cgttctat
//
LOCUS       DAOPBK010000080        11276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5659_length_11276_cov_5.045361, whole genome shotgun sequence.
ACCESSION   DAOPBK010000080 DAOPBK010000000
VERSION     DAOPBK010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5659_length_11276_cov_5.045361"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLA17_06105"
     CDS             <1..305
                     /locus_tag="PLA17_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPK85023.1"
                     /translation="DTAVVSFVISGTGPEINENQVILHPNPFTEEFSLEVDFEGSETA
                     NFELIDTSGRIVMRSVAELSPGRNRIPFNCRHLGSGLYTLRVSYGSRSFSMKAVKK"
     gene            351..941
                     /locus_tag="PLA17_06110"
     CDS             351..941
                     /locus_tag="PLA17_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPK85024.1"
                     /translation="MTGKIPTREEALELFRRYNKSDSLYKHALSVEAVMRYMARKHGE
                     DEEKWGIIGLVHDLDYEMYPDQHCTMTEKILKEEGWPEEYVRAVVSHGYGICSEVEPL
                     TLLEKTLFAIDELTGLVTTSALVRPSRSVLDMEAKSVRKKWTDKRFAAGVDRSVIEKG
                     AAMLNVDLNDLISDTISGMREVAEDIGLYGNPAPGA"
     gene            complement(1141..1887)
                     /locus_tag="PLA17_06115"
     CDS             complement(1141..1887)
                     /locus_tag="PLA17_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK85025.1"
                     /translation="MKLIKTMFVLLLAVSFVSCGTGKGKKDAEKKSTEAEVWTFLFDG
                     SGTHGWRNYNKTTFPEQGWEVVDGTLHCIGSGRGEAGGGGGDIIYDKKFSNFHLKLEW
                     KISEGGNSGIFYLAQEGPEWDYIYKTAPEMQVLDNERHPDAEAGKDGNRQAGSLYDLI
                     PANPQNAKPAGEWNTAEIKVYKGSVWHIQNGETILEYHLWTPEWNNLVAGSKFPALNP
                     DWANVASEGYIGLQDHGDDVWYRNVQIREL"
     gene            complement(1959..3458)
                     /locus_tag="PLA17_06120"
     CDS             complement(1959..3458)
                     /locus_tag="PLA17_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK85026.1"
                     /translation="MKEETKKITRRSFVGTTGAAFAGLTILPGNVVSGLGHRAPSDKL
                     NIAAIGIGGMGASNLRNVAATENIVALCDVDWGYSKRVFEAYPAAKRFWDYRKMYDEM
                     GKSIDAVIIATADHTHAITAAHAMTMGKHVYLQKPLTHTVYESRLLTRLAAKHKVATS
                     MGNQGSSAIGVDLVQEWIQNREIGEVTKVEAFTDRPIWPQGMNRPAKVDKVPKTLDWD
                     LFLGPAPVRPYNAIYHPWNWRGWWDFGTGALGDMACHILHPVFKSLRLKYPTKVQASS
                     TLLLTDCAPNAQTVKYIYPARTAPSHYKIDLPEVEVIWYDGGLQPMKPEGWPEGKDMN
                     DSGGGVIFHGTKDKLICGCYGINPWLLSGRVPNAPVTERRVENATRGGHEMDWVRACK
                     ESPENRIPTKSDFAEAGPFNEMVVMGVLAVRLQGLNKILEWDGEKMEFTNIKDDETIK
                     ICIEDNFTITDGHPTFNKKWTDPIIAKQFATEMVRHTYRDGWSLPEMPA"
     gene            3611..4108
                     /locus_tag="PLA17_06125"
     CDS             3611..4108
                     /locus_tag="PLA17_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85027.1"
                     /translation="METQRFSLDTGEHDRISLIARTIFGVVCIAAAIITTVKMAGSGL
                     LNMNNIAAIVFLGLFGIWLLATGLGLTQRHMTLAGDSIVLKKSAYSPARTIKAADIRE
                     IEFSQLRITFTLKTGKIIALQLGLLYRESSLRLMEAVEQFCTSNGIVARGIESDMNNT
                     IHEEN"
     gene            4095..5246
                     /locus_tag="PLA17_06130"
     CDS             4095..5246
                     /locus_tag="PLA17_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK85028.1"
                     /translation="MKKIRYGMIGGGEGSLIGPVHRMAARLDALCELVSGAFSSNPEI
                     SRLTGTREALNEARIYDSWQEMIIAESALPDEIRPHFITIVTPNHLHYGPAKMALEAG
                     FHVICDKPLCLSVTEALDLQETVDRTGKLFCLTHNYTGYPMVKAARNMVLSGEIGKTR
                     KVIVQYLQGWLSTPLEKSGNRQAEWRSDPARAGIAGAMADIGTHAFNLAEYVTGEEVV
                     SLSAELHRVVAGRMLDDDGTVSLTFGNNISGTLLASQVATGEENNLSIRIYGEDGALI
                     WRQMEPNTLIHLRSDAPVQVIRTGTSFAVTGLPAFMHTRLPAGHPEGFIEAFGNLYRN
                     FAMHIKAVNDGQKPDPAADYPGIKEGVRGMKFLEAVVSSSRNGSTTTGL"
     gene            complement(5401..6252)
                     /locus_tag="PLA17_06135"
     CDS             complement(5401..6252)
                     /locus_tag="PLA17_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPK85029.1"
                     /translation="MTTRRDFLRKSALVIAAAPLINSEMIASPKPSKKTGLALYTIRG
                     EMGKDPAAALAAVAETGFTWLEAADHRDGKFYGMKPKEFGKLVKKNGMVALSSHSGIN
                     PENYEKMIDEAAESGMKYIILPSLPRDWTNTIDGYQRAADFFNRAGAKCRKAGITFGF
                     HNHQVEFQEINGRVPFDILVEQSDPKLVIFEMDLAWITAAGKDPVAYFRKYPGRFELW
                     HLKDLTPEKQDATLGEGIIDFRPILAEARTAGMKYWFLEQDFCRTHTPMESIRISRDY
                     YLRLLEK"
     gene            6361..7362
                     /gene="amrS"
                     /locus_tag="PLA17_06140"
     CDS             6361..7362
                     /gene="amrS"
                     /locus_tag="PLA17_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPK85030.1"
                     /translation="MRFWEKHKEGSVRCTLCPHFCIIRHGERGICGTRENRDGVIYPV
                     TAGIISGYAIDPIEKKPLYHFHPGSEILSVGSFGCNLSCDFCQNYHISQNVEGEESAR
                     LDPAELVRQAERARGNIGIAYTYNEPVIWFEYVTECARLAREKGLCNVMVTNGYVNPE
                     PLKELTEVIDAFNIDLKAFSNDFYRQYSGATLKPVLETIKAVARSGRHLEITTLILPG
                     MNDSPDEMKREAEWIAENAGSSVPLHLSRYFPMYRRTTPATPPETILRLREIAENYLD
                     FVYTGNMHDEEGGSDTRCPGCHTTVIKRSGYHTRTSGLTDEGSCLKCSRQIIAETNI"
     gene            complement(7376..7666)
                     /locus_tag="PLA17_06145"
     CDS             complement(7376..7666)
                     /locus_tag="PLA17_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85031.1"
                     /translation="MASIRSLKKDIDFLFFELISDCFLATSIHEGEKKIEIQNLIEEA
                     VVLRYEFIDRANHHPEGKDNPALVRAYYTNLRKDLSGKVNEYFEKLSSIAKQ"
     gene            complement(7697..8533)
                     /locus_tag="PLA17_06150"
     CDS             complement(7697..8533)
                     /locus_tag="PLA17_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPK85032.1"
                     /translation="MDCTGNYIWLDGAIVAVSDIEEFPSGEVSFYEVIRTRAGVPLFF
                     SDHMNRLNDGIATRYELQDDIVAEVRAGMKALAKHERHDEINVRVTVTFSGYDHTLHI
                     CYIPSSYPSETMIREGVPLILYHAERLDPGVKTMNNRLRLSVSSELAVRNAYEALLVN
                     RDGFITEGSRSNIFFVAENGAIRTAPDSMVLSGITRRYVTDIIRKEGIRLVYEAVKES
                     DAGRFRSAFITGTSPMVLAVRSIEGLQFDVADPLIGRLREAYSLLVEQSMSDYKMEDE
                     AD"
     gene            8613..9977
                     /locus_tag="PLA17_06155"
     CDS             8613..9977
                     /locus_tag="PLA17_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPK85033.1"
                     /translation="MYAIVDIETTGGSARLERITEIAVYIHDGNRIVEEFSTLINPER
                     NIPCFITSLTGITNEMVEEAPKFYEVARRIVELTEGKIFVAHNARFDYSFIRQEFNML
                     GYNFKRPILDTVALSRKLIPGHSSYSLGNLCNDLGIEINGRHRASGDALATVRLLELL
                     LEKDRELNSGSLIKNRKAAKLHPALDIAKLEEIPEEPGVYYFHDEGGDVIYVGKSRNL
                     SERVSSHISNNTSAREMEMRSMIADITWERTGSELIALLMESAEIKSRKPRFNRAQRR
                     TGFRHGIYSETDEAGYIRFSYRNVRDEEVPLAIFTSRDRARGKLEQIVTDYNLCQKLC
                     GLYETSGPCFHRQVSICRGACCGEEDPESYNERALMALDEFIFRERNFFIIDRGRDEE
                     ERSAVKIVKGKYAGYGFFSINDVGFGLTALHDCIKPAHDNRDIQVIIKGYLKKHRVER
                     IIDF"
     gene            10036..10686
                     /locus_tag="PLA17_06160"
     CDS             10036..10686
                     /locus_tag="PLA17_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPK85034.1"
                     /translation="MSLFTRTNRELSIRIDEFFDTIEIGILIFREGIKAYVNGDTNAF
                     QNHLAKIDDLEGKADKLQRSIENDMIIHSILPQQRSEMFKLLGQLDEIIDTAKKSLNE
                     FYVEIPRIPASLSHEFISLSEVSGNSAEELMPAARAFFREPHLVREKLIKVYFFERET
                     DKLAFQIKKKVFHEMNELSLAEKAHIRYIIHHIENISDTAQDAADLLASMAIRQML"
     gene            10689..>11276
                     /locus_tag="PLA17_06165"
     CDS             10689..>11276
                     /locus_tag="PLA17_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPK85035.1"
                     /translation="MILLFLTSGLFLGWTLGANDASNVFGSAVGSRMVSFTRAAVTAA
                     VFVIIGAVVQGSGAAETLSRLGEVNAMGGAFTVALAAGLTVYFMTRAGLPVSTTQAIV
                     GAIIGWNFFTNNAVDSGVLTKIISTWITGPVLGAIFAYALYHLVKTARRRIKIHLLKY
                     ENWLRIGLMTVGAFGAYSLGANNIANVMGVFVPSIP"
BASE COUNT         2875 a         2805 c         2774 g         2822 t
ORIGIN      
        1 atgataccgc tgtcgtctcg tttgtcatat caggaaccgg accggaaatc aatgaaaatc
       61 aggtaatcct gcatcccaat ccttttactg aggagttctc gctggaagtt gactttgagg
      121 gaagtgagac ggcaaatttt gaactcattg atacttccgg aaggatcgtc atgcggtcgg
      181 tggctgaact gtcgcccggc aggaaccgga ttccgttcaa ttgccggcac ctcggatcag
      241 gcctctatac gctacgggtc tcatacggga gcaggagctt ctcgatgaag gcagtcaaaa
      301 agtaacccta cgggatattc caattgcatt tatcacaata atatcaggaa atgacaggca
      361 aaatacccac cagggaagaa gctctggagc ttttcagaag gtacaacaag tcagacagcc
      421 tctacaaaca cgctctttca gttgaggctg taatgagata catggcgcgc aagcacggcg
      481 aagatgagga aaagtgggga atcatcggac tggtgcatga tctcgattat gagatgtacc
      541 ctgaccagca ctgcaccatg acggagaaga tacttaagga agagggatgg ccggaggagt
      601 atgtcagagc tgtagtcagc catggatatg gcatctgctc tgaggtcgag ccccttactc
      661 tccttgagaa aacgctgttt gcaattgatg agctgaccgg actcgtgacc acgagtgcac
      721 tcgtcaggcc ttcacgaagc gtgcttgaca tggaggcaaa gtcagtccgc aaaaaatgga
      781 cagacaaaag gttcgccgca ggtgttgacc ggagtgtcat cgaaaagggt gcagccatgc
      841 tcaatgttga cctgaacgac cttatctcag acacaatatc aggtatgcgg gaggtggccg
      901 aggatatcgg attgtatggg aatccggcac ccggggcgtg atatatacat atgatatctg
      961 cctgccccgg gggtaattat ctgtatatgg gtacgtaccg acgggtctga gacccgtcgc
     1021 cacctgcccc cggggtaata tctgtatatg gacacatagc ggcgggtctg agacccgccg
     1081 ctagccctca gggataatat ctacatattg ggtaattatc tatcctgatg atgatgatga
     1141 ttacaattcc ctgatctgga cattacgata ccagacgtca tcgccgtgat cctgcaggcc
     1201 tatgtaaccc tcggaggcaa cattggccca gtcgggattc agtgcgggga atttgctgcc
     1261 agcaacaaga ttgttccact caggcgtcca gagatggtat tcgagaatgg tctcaccatt
     1321 ctgaatgtgc catacagatc ccttgtaaac cttgatctca gctgtgttcc actcacctgc
     1381 cggcttggca ttctgcgggt ttgcagggat aaggtcatag agtgaacctg cctgacggtt
     1441 tccgtccttg ccagcctcgg catcagggtg cctttcattg tcaagaacct gcatctcggg
     1501 agcagtctta tatatataat cccattccgg gccttcctgc gccaggtaga aaattccgct
     1561 gtttccgcct tctgatatct tccattccag cttgaggtgg aagttcgaga atttcttgtc
     1621 atagatgatg tcaccgccac caccgcctgc ttcgcccctt ccggaaccta tgcaatgcag
     1681 agtgccatca acaacttccc atccctgttc gggaaaggtg gtcttgttgt agttacgcca
     1741 gccatgggtt cctgatccgt caaagagaaa agtccatacc tcggcttcag tcgatttctt
     1801 ttcagcatcc tttttcccct ttcctgttcc acaggagaca aacgagactg caaggagaag
     1861 aacaaacatt gtcttaatca gtttcataag ttattaattt taagtatttg ttttgatttt
     1921 caattcagca aaaccaacct gcctgtcaga ttttaacatt atgcaggcat ttcgggaagt
     1981 gaccagccgt cacgataggt atgcctgacc atttcggtgg caaactgctt cgcaatgatc
     2041 ggatcggtcc acttcttgtt gaatgtcggg tgaccgtcag taatggtaaa attatcctcg
     2101 atacagatct ttattgtctc atcatctttg atattggtaa actccatctt ttccccgtcc
     2161 cactcgagaa tcttgttcag tccctggagc cttacggcca ggacacccat tactaccatt
     2221 tcattgaacg gaccggcttc ggcaaaatca gacttggtgg gaattctgtt ttcggggctt
     2281 tccttgcatg ctcttaccca atccatctca tggcctcccc tggtggcatt ctctacacgt
     2341 ctttcagtta caggagcatt tggcaccctg cctgagagga gccatggatt aatgccatag
     2401 caaccgcata tgagcttatc cttggtacca tggaagatga ctccgccccc cgagtcattc
     2461 atatcctttc cttccggcca tccttcgggc ttcataggct gaagtccacc gtcataccat
     2521 atcacctcca cttcaggcag gtcaatcttg taatgtgatg gcgccgttct ggcaggatag
     2581 atatacttca cagtctgtgc gtttggagca cagtcagtca gcagcagggt tgagcttgcc
     2641 tgtacttttg tcgggtattt aagcctgaga gatttgaaga caggatgcag gatatggcat
     2701 gccatatcac ccagcgcgcc ggttccgaaa tcccaccatc ccctccagtt ccagggatga
     2761 tatatggcgt tgtaaggcct gacgggagca ggtcccagga acaggtccca gtcaagtgtc
     2821 ttcggcacct tatctacttt cgcaggccgg ttcatgccct gaggccatat cggacggtca
     2881 gtgaaggctt ctaccttcgt aacctcacct atttcgcggt tctgtatcca ctcctgtaca
     2941 aggtctactc cgattgctga ggagccctga ttacccatcg atgttgccac tttatgcttc
     3001 gctgcaagtc tggtcagaag tctcgattca tatacagtgt gggtaagggg tttctgcagg
     3061 tagacatgct tgcccatggt catcgcgtga gcagcagtaa ttgcatgagt gtggtctgcc
     3121 gttgcaatga tgacggcatc aattgacttg cccatctcat catacatctt ccggtaatcc
     3181 cagaatcgct tcgctgccgg ataggcttca aagaccctct tcgagtagcc ccagtccacg
     3241 tcacaaagtg cgactatgtt ttctgttgct gcaacattcc tgagatttga ggcacccata
     3301 cctccgatac caattgcggc gatgttcaac ttgtcactgg gcgcccggtg gccaagcccg
     3361 ctgacaacgt tgccgggcag aattgtaagt ccggcaaatg cagcacctgt agtgcctaca
     3421 aatgaccgtc ttgtgatttt ttttgtctcc tctttcatat catttcaggt taagttcttc
     3481 aatggttgga taaaaataga aatattctcc caagtttact atttctttca tatattttag
     3541 ggcatgatgc ctgacaggta caacaatcag gagttgttgt gaaaatatac attaccttgc
     3601 agaaaaacat atggaaactc agagattcag tcttgatacc ggtgagcatg acaggataag
     3661 cctgattgca agaaccatct ttggggtagt ctgtattgcc gccgcaataa taaccacagt
     3721 taagatggcc ggctcaggac ttctgaatat gaacaatata gctgccatag ttttcctcgg
     3781 tcttttcgga atatggctgc tggcaacggg tcttggcctg actcaaaggc acatgactct
     3841 tgccggtgac tcaatcgttt taaaaaagag cgcatactca cctgcgagaa caataaaggc
     3901 tgcagatatc agggagattg agttcagtca gctgagaata acattcacac taaagaccgg
     3961 gaagattatt gcactacaac tgggactcct ttacagggag agctcattac ggctgatgga
     4021 agctgttgaa cagttctgca cttcaaacgg aatcgttgcc aggggaatag aatctgacat
     4081 gaataatacc atccatgaag aaaattaggt atggaatgat cggcggaggc gagggatcgc
     4141 ttatcggacc cgtgcaccgc atggcagcac gtcttgacgc tctctgtgaa cttgtttccg
     4201 gtgcattcag cagtaacccg gaaatctcac ggctgacagg aaccagggag gctctcaatg
     4261 aagcacgtat ttatgacagc tggcaggaga tgatcattgc cgaaagcgcc ctgcccgatg
     4321 agatcagacc ccacttcatc accattgtca ccccgaacca tcttcattat ggtccggcaa
     4381 aaatggctct ggaggcagga tttcatgtta tttgtgacaa acccctgtgt ctcagtgtga
     4441 ctgaagctct tgacctgcaa gaaacagttg accggacagg gaaactgttt tgcctgaccc
     4501 ataattatac cggttatccc atggttaagg cagcacgcaa tatggtgctt tcgggagaga
     4561 tcggaaagac caggaaggtg attgttcagt accttcaggg atggctctca acaccgctgg
     4621 aaaagagcgg taaccggcag gctgaatgga ggtctgaccc ggcaagggca ggcattgccg
     4681 gggcgatggc tgacataggc acacatgcat tcaaccttgc ggaatatgtc acgggcgagg
     4741 aggttgtctc attgtcggca gaactgcaca gagtggtagc cgggaggatg cttgacgatg
     4801 atggcactgt ctcgctcact tttggaaaca acataagcgg aacccttctt gccagccagg
     4861 tggccaccgg agaggagaac aacctgtcaa taaggattta cggtgaagat ggagctctta
     4921 tatggcgtca gatggagcca aatactctca tccatctccg ctctgatgct cctgtgcagg
     4981 ttatccgtac cggcacctcc tttgccgtaa cgggtttacc cgccttcatg cacacaagac
     5041 ttccggcagg gcaccctgaa ggattcattg aggcattcgg caacctgtac aggaattttg
     5101 caatgcacat caaggcagtt aacgatggtc agaaacctga tcctgcagct gactaccccg
     5161 gaatcaaaga aggggtcagg ggaatgaaat ttctggaggc ggtggtctct tcttcacgga
     5221 acggatcaac cactaccgga ctctgagaag aataaagaag ccccccgggc acaggacacc
     5281 ggagagctgc attaaatctg tgccggaact gatttttcgg actcttcttt ttttagaagt
     5341 ccgaggtaga tttttcggac tcttcttttt tcagaagtcc gaggttgatt ttccaggctc
     5401 ttatttttcc agaagtctga ggtaataatc cctgcttatc ctgatgcttt ccatcggagt
     5461 atgcgtgcgg caaaagtcct gctccaggaa ccagtatttc atgcctgctg tcctggcctc
     5521 agcaagaatc ggcctgaaat cgatgattcc ctctccaagg gttgcatcct gtttttccgg
     5581 ggtaaggtcc ttcagatgcc ataactcgaa ccggccggga tactttctga agtatgccac
     5641 cgggtccttt cctgcagctg ttatccatgc caggtccatc tcaaagatta ccagcttcgg
     5701 atcactctgt tccacgagga tatcgaaggg cacacggcca ttgatctcct gaaactccac
     5761 ctggtggttg tggaacccga aggtgatgcc ggctttcctg cacttcgccc ctgccctgtt
     5821 aaagaaatcg gctgccctct ggtaaccgtc gattgtgttg gtccagtcac gggggagcga
     5881 tggcagtata atatatttca tccctgactc agctgcttcg tcaatcatct tttcgtagtt
     5941 ctcagggttg ataccgctgt gtgagctgag tgcaaccatt ccgttcttct ttaccagctt
     6001 gccgaattcc ttcggcttca tgccatagaa ctttccgtca cggtggtcag cagcctcaag
     6061 ccatgtgaac cccgtttccg caacagcggc aagcgcagct gcagggtcct tgcccatttc
     6121 gccacggata gtgtacaatg caagtccagt cttctttgaa ggctttggtg atgcaatcat
     6181 ctcgctgtta atcagcggtg ctgcagcaat tacaagggct gatttcctta aaaagtctct
     6241 tctggtagtc attttttcag gttattgttt aacggatcat gaagttatct ccccaatcta
     6301 accaatgatg aaagagaaca aaaataacat taaatttata agtttgaaaa acagatgcag
     6361 atgaggtttt gggagaaaca taaggaagga agcgtgagat gtaccctgtg cccgcatttt
     6421 tgcatcatca ggcacggaga gagaggtatt tgcggaacca gggaaaaccg ggatggagtg
     6481 atttatccgg ttaccgccgg catcatctca ggatatgcca tcgatcccat tgaaaagaaa
     6541 ccactctacc actttcaccc ggggtcagag atactgtcag taggatcatt cggatgcaac
     6601 ctcagttgcg atttttgcca gaactatcac atttcacaaa atgttgaagg ggaagaatct
     6661 gcaaggctcg atccggcaga gcttgtccgc caggctgaaa gagcccgggg taacattggc
     6721 atcgcctata cctacaatga accggtgata tggtttgaat acgtaactga atgcgccagg
     6781 cttgcccgtg agaagggtct atgcaacgta atggtaacca atggctatgt aaatccggag
     6841 ccgttgaagg aactgacaga ggtcattgac gccttcaaca tagacctgaa agcgtttagc
     6901 aacgacttct acaggcaata ttcgggagcc actctcaagc cggtgctgga gacaatcaaa
     6961 gctgtggccc ggtcaggcag gcacctggag ataaccaccc tgatccttcc ggggatgaac
     7021 gactcgcctg atgagatgaa acgtgaggct gagtggatcg ccgaaaatgc tggcagctcc
     7081 gtcccgctgc acctcagccg gtacttcccg atgtacagga gaaccacacc tgcaacgccg
     7141 ccggaaacaa tactaaggct cagggaaata gcggaaaact atctcgattt tgtgtatacc
     7201 ggcaacatgc acgacgaaga gggtggcagt gacaccaggt gccccggctg ccataccaca
     7261 gtgataaaaa gatcaggata ccatacccgg acttccggtc ttacggatga gggaagctgc
     7321 ctgaaatgca gccggcagat cattgccgaa acgaatatct gaggcgcaga cggaactact
     7381 gtttcgcaat actgctaagc ttctcgaagt attcattgac cttgccggac agatccttac
     7441 gcaggttcgt ataataagcc cttacaaggg ccggattatc tttcccttca ggatgatgat
     7501 ttgccctgtc aatgaactca tagcgcaaaa ctacagcctc ttcaatcaga ttctgtatct
     7561 ctatcttctt ctctccttca tgaatacttg tagccaaaaa acaatcagag atcagctcga
     7621 agaagagaaa atcgatatcc tttttcagac ttctaatact tgccattgat ggtttgtttt
     7681 gatgcaaata tattgattaa tccgcttcat cctccatttt gtaatcactc atgctttgtt
     7741 cgacaaggag cgagtaagcc tcgcgaagtc ttcctatgag cggatcggcc acgtcaaact
     7801 gcagaccctc gatgcttctc acggcaagca ccatcggcga ggtgccggta ataaaagccg
     7861 atctgaatct gccggcatcg ctctccttta cggcttcata aacaagcctg attccctcct
     7921 tccggatgat atcagtcaca tatctcctgg taatccctga aaggaccatg ctgtcaggtg
     7981 ccgttcgtat cgccccgttc tccgcaacga agaagatgtt tgacctgctc ccctctgtaa
     8041 tgaagccatc acggtttacc agcagcgctt cgtatgcatt ccttactgca agctcactgc
     8101 tgaccgagag acggagcctg ttgttcatag tcttaacccc cggatcaagc cgttcggcat
     8161 ggtagaggat caacggcact ccctctctga tcatcgtttc cgacggatag gatgaaggaa
     8221 tatagcagat atgcagtgta tggtcatatc cgctgaaagt aactgtcacc ctgacattta
     8281 tttcgtcatg ccgttcatgt ttggcaagcg ccttcattcc tgcccttacc tctgccacga
     8341 tatcatcctg aagttcatac ctcgttgcaa tgccgtcatt gaggcggttc atgtgatcac
     8401 tgaagaagag agggactccg gcacgggttc tgatcacctc gtagaaggag acctctccgg
     8461 atggaaactc ctcaatatct gatactgcca caatggctcc gtcaagccat atgtaatttc
     8521 ctgtgcaatc catatccgga tttctgtttt tatcaaaaat aggaataatg tagcagcctt
     8581 aaaagtaatt atcttagtgt tttccggaag agatgtatgc cattgtagat atagagacaa
     8641 caggtggtag cgcgagactt gagagaataa ccgagatagc cgtttacata catgacggca
     8701 acaggatagt ggaggaattt tcgaccctta tcaatcccga gcgcaatatt ccctgcttca
     8761 ttacttccct tacaggtatc accaatgaga tggttgagga ggcacccaaa ttctatgagg
     8821 tggcacgccg gattgtagag ctgacggaag gaaagatatt cgtggcacac aatgccagat
     8881 tcgattattc attcatcagg caggaattca acatgctggg ttacaacttc aagcgcccga
     8941 tacttgatac cgttgccctg agccgcaaac tcattcccgg gcacagctcc tacagcctgg
     9001 gaaacctttg caatgacctc gggatagaga tcaacggccg gcacagggct tcgggcgatg
     9061 cccttgctac agtgaggctg cttgagctgc tgctcgagaa agaccgtgaa ctgaattccg
     9121 gcagcctgat caaaaacaga aaggcagcga aactgcaccc tgcacttgac attgcaaaac
     9181 tggaagagat ccctgaagaa cccggagtgt actatttcca tgatgaagga ggagatgtaa
     9241 tatatgtggg aaaaagccgt aacctgagcg agcgtgtcag ctcacacatc tcaaacaata
     9301 cctctgcaag ggagatggag atgaggtcaa tgattgctga catcacatgg gagcgcacag
     9361 gaagtgagct gattgccctg ctgatggagt cagccgagat aaagagccgt aaaccccgct
     9421 tcaacagggc acagcgacgc acggggttcc gccatggtat ctacagtgaa acggatgaag
     9481 ccggatacat cagattctca tacaggaatg tgagagatga ggaagtgcct cttgcaatat
     9541 tcacctcacg tgacagagcg cggggcaagc tggagcagat tgtcactgat tacaacctgt
     9601 gccagaagct ctgcggattg tatgaaacat ccggaccctg cttccaccgc caggtgagca
     9661 tatgccgcgg ggcctgctgc ggagaagagg acccggagtc gtacaacgaa agagcactca
     9721 tggctcttga tgagttcatc ttcagggaga gaaacttctt tatcattgac cgtggtcgcg
     9781 atgaggagga aaggtctgcc gtcaagatag tcaaaggcaa atatgcaggc tacggcttct
     9841 tcagcattaa cgacgtgggg ttcgggctga cggcattgca tgactgcata aaacccgctc
     9901 atgacaatcg cgacatccag gtcatcatca agggttacct taagaaacac cgggtggaaa
     9961 gaattattga tttctgactc ctcggagtgc tgccttttct tatatatatt tgcctgaaac
    10021 aacacaagcc cgtatatgtc actatttacc cgcaccaaca gggaactcag cataagaatt
    10081 gatgaattct ttgataccat agagattggt attctgatct tcagggaggg catcaaagca
    10141 tatgtcaacg gtgacacaaa tgccttccag aaccacctcg cgaagatcga tgatcttgaa
    10201 ggcaaggccg acaaactgca gcgcagtatt gagaacgaca tgataattca ttcaatcctg
    10261 ccgcagcagc gaagtgagat gtttaagttg ctcgggcagc tcgatgagat tattgacacg
    10321 gcaaagaaat cgcttaatga gttttatgta gagatacccc gcatacctgc ttcactgtca
    10381 cacgagttca tttccctctc ggaggtgtct ggcaactcgg ctgaggagct tatgccagct
    10441 gccagggctt tcttccgtga gcctcacctg gtgcgtgaga agctcatcaa ggtctacttc
    10501 ttcgagaggg agacggataa gctggccttt cagataaaga aaaaagtctt tcatgagatg
    10561 aatgagctct cactggctga aaaggctcat atcaggtaca tcatacacca tattgaaaat
    10621 atctcagaca cggctcagga tgcagctgac ctcctcgcct ccatggccat aaggcaaatg
    10681 ctctgatcat gattctcctc tttctcacaa gcggcctctt cctgggatgg acactcggcg
    10741 ccaacgatgc ttccaacgtg ttcgggtctg ccgttggctc aaggatggtc tcctttaccc
    10801 gggctgcagt tacagccgct gtttttgtta ttattggcgc tgttgtccag ggcagtggcg
    10861 ccgctgaaac gctgagccgc ctcggagaag taaatgcaat gggcggcgct tttacagtgg
    10921 cgctggcagc cggattgacc gtatacttca tgacaagggc aggtctgccg gtatcaacaa
    10981 cacaggcgat cgtgggtgct atcataggct ggaacttctt taccaacaac gcagtcgaca
    11041 gcggggtact gacaaagatt atatctacct ggattacggg acctgtgctc ggagcaatat
    11101 tcgcatatgc actctaccac ctggtgaaaa cagcaaggcg acgtatcaaa atacacctgt
    11161 tgaaatatga aaactggctg cgcattggtc tgatgaccgt tggcgcattc ggcgcctaca
    11221 gtcttggggc aaacaacata gccaacgtta tgggtgtatt cgtgccaagc atccct
//
LOCUS       DAOPBK010000081        11208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5695_length_11208_cov_6.114588, whole genome shotgun sequence.
ACCESSION   DAOPBK010000081 DAOPBK010000000
VERSION     DAOPBK010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5695_length_11208_cov_6.114588"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..2858)
                     /locus_tag="PLA17_06170"
     CDS             complement(39..2858)
                     /locus_tag="PLA17_06170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK85036.1"
                     /translation="MNIKRILFNVTLLTIVAIFMAACGKTGQEDKLNLSYEKYTMPNG
                     LQVVLHQDHSDPVISFAIMYHVGSSRETPGRTGFAHLFEHLLFSGSENVPNGVFDMVI
                     ENAGGSNNGFTSRDVTAYFESFPKNALEKILWLESDRMGFFINSVTPRNLAIQQNVVQ
                     NEKRQGEDNSPYGFTNYVISKNLYPESHPYSWEVIGEMEDLANAGVDDVREFYQNFYG
                     PNNATLVIAGDFEADSVKLLLDKYFGEIKSHGEVTARTAMPVTLTETKRLYHEDNFAN
                     VPEINMVWPVPENYTKDSYALSFLSELLSSGKKAPMYKVLVKEKQLTSRVSAYNSAEE
                     LAGEFTISVTANEGKSLKEIEEAINEAFARFEADGFTDRELERIKATMEKSFYQGLTS
                     VMNKAIRLASYNTILNDPGFFAKDIENIRAVTRDDIMRVYEAYIKGKPHIVTSFVPKG
                     QQEMMAENSVRAAVSEEDITKASQVAIEEMDETPVEKTASAIDRTIEPAAGPEPEVRV
                     PEVWRATLKNGIRVYGTENRELPLVSMSVVIAGGAHGDDISMPGVASMVAAVLPQGTR
                     NRTPEELEEEIELLGSSVVMRASGEEITLSGSTLSRNFAATVSLMKEILLEPRWDTAE
                     FIMAQTRTRNGLIQAEARPASVASEQFMKLVYGPEHIMGYDLRGTKESVEQITPEILK
                     SFYETNFSPSVTSIYVAGNVSRKEVLNALKPLEAEWQAREVPARSYELPAAPEESQIY
                     FFDIPGSRQSVVYAGYIAMTRNDPDWVKANFANYRMGGAFTSILNQILREQKGFTYGA
                     RSSFREMKIPAPFIISTSVRSDATLETLEIITGELAKYREGISEEELQFIKNSMIRSN
                     ARRFETNDAMAAMLTYIGKYGFSDDWVRKEEDVIRNMTVEDHKAITDKYFDPGRMYYV
                     VVGDAATQMKQLEKVGFGKPVLLER"
     gene            complement(3028..5727)
                     /locus_tag="PLA17_06175"
     CDS             complement(3028..5727)
                     /locus_tag="PLA17_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPK85037.1"
                     /translation="MTKKIVLSLALVTLCLTLPAQVSVTGLLTENMTNPVGIDAAQPR
                     FSWQLVSYKRNTVQTAYELRVSEGTRPVWNTGRVNSDQSVHIVYAGEPLASGRDYNWQ
                     VRVWDNGGKRSAWSPPAKFRTGIIGKNWWKAKWIEPGYAEDTVMRPGPMMRKEFTVSG
                     KIVRATAYITAHGLYEAELNGKRIGDAFLTPGWTSYGKRLQYQAYDVTHLLKNGSNAI
                     GVMLGSGWYRGNIGFRSSFNFYGNDIALLCQIDITLSDGSTESIISDGSWKSSTGPVR
                     YSEIYHGEIYDARMEKKGWTLPGYDDTGWDGVKEAAHSMDVLIATQNEPVKKHETFVP
                     VQIMETPAGDQVIDFGQNLVGWVQMRVRGNAGDSVILSHAEVLDRNGNFYTANLRSAK
                     ARDIYVLKGGGEEFWEPRFTWHGFRYLKVEGYPGKLTPGDFTAVALYSAMRPTGSFVT
                     SDSLINRLQHNIQWGQKGNFLDIPTDCPQRDERLGWTGDAQAFSRTAAFNMNVNNFFA
                     KWLKDLEADQVDGKVPHVVPNILGAGSVNSTGWADVATIAPWNMYLVYGDRQLLEQQY
                     GSMKAYVESIRSTARDDLWNSGFHYGDWLFYRPDDDNSGMAAVTDKYLIAQCFYANSV
                     QLLIKAANVLGKRDDADSYGKLLERVRDAFMKEYVTPNGRLVSGTQTAYVLALNFDML
                     PVDLREQAARRLAENVKSYGHLTTGFLGTPYLCHVLSRFGYTDLAYELLLRKKYPSWL
                     YPVTMGATTIWERWDGQKPDGSFQTPGMNSFNHYAYGAIGDWMYRVIAGVDTREEGPG
                     YKKIIIKPHIGGGLTSVSASLETYYGLLGCTWRINNECFEMDVVIPANTTATVCIPAE
                     DPAEIYEGGLPLAQKKEIKVEGNEDGYVIMTVGSGKYSFRRNR"
     gene            complement(5731..7095)
                     /locus_tag="PLA17_06180"
     CDS             complement(5731..7095)
                     /locus_tag="PLA17_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPK85038.1"
                     /translation="MDVIAVFDIGKTNKKFLLFDLNLILIHQEEKVFGEIRDDDGFES
                     DDIEGIVSWMKSRLAEAVRSEDYQVIALNFSSYGASLMHLDHQGERVTPLYNYLKPMP
                     EGLTDEFYEAWGGVEEFSRITASPALGMLNSGLQLLWLKKMKPALFARIRTTLHLPQY
                     LSRIFTGREASEYTSVGCHTAMWDFEKMQYHAWLKAEKIKLPDPVVPSGVADVNIEGR
                     TVRTGIGLHDSSASLVPYLKSSDRPFILVSTGTWCIFMNPFNTEPLTADQLRNDTLCY
                     LSIDSQQVKASRVFLGHIHDMNVARLDDHFGVTDELYKTIKIKSQKIEKLLASGNGRV
                     FFRQGIPPGYVDEQADLSHYLTYADAYHQMICDLVDLCMESWRLIIPADDKTEIVYVT
                     GGFARNDTFVRFLAARLPDKRVYASQVENSTALGAAMTIYESAVGKSLPPVYLGLKAI
                     IDND"
     gene            complement(7102..8370)
                     /locus_tag="PLA17_06185"
     CDS             complement(7102..8370)
                     /locus_tag="PLA17_06185"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HPK85039.1"
                     /translation="MKKEKVLQAYQDAREQYAALGVDTDEALRRLDSISISLHCWQTD
                     DVGGFETPDATLSGGGIQATGNYPGKANSVSQLRQDMEKVMSLLPGSQRVSLHAMYGE
                     FGGTKIDRDSITTDHFQGWIDWAKNLGTGLDFNPTFFSHPLAEDGFTLSGRNEKNRRF
                     WIEHAKRCRRIAAEMGRQLGTPCVNNLWIPDGSKDTPVDRYALRALLKESLDEIFSEE
                     FPAEYLRDALESKLFGIGSESMVVGSAEFYMGYAIKNNKLITLDNGHYHPTEQIGDKI
                     SSLLLYVDELMLHLTRGVRWDSDHVVIFNDEIQLIAQEIVRCKALERVHVGLDYFDAS
                     INRIGAYVVGTRAAQLAFLHALLEPTETLRKYEEEGRYFERLALMEVMKTKPLGAVYD
                     YWCLTGNVPVAEDYIVEIQKYEKEVLSERT"
     gene            complement(8393..9424)
                     /gene="rhaT"
                     /locus_tag="PLA17_06190"
     CDS             complement(8393..9424)
                     /gene="rhaT"
                     /locus_tag="PLA17_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HPK85040.1"
                     /translation="MNPNPILSIIIMSIGSMCAASFYVPIRKVRGWSWESYWIVQGLF
                     SWIIVPLLFAWITVPAGTLGAIISQAPASSKWWAVFYGALWGIGGLTFGLSMRYLGVG
                     LGQSVALGLCAAIGTLIPPVISGQNLFADRSGILMLTGVSVSLAGIAILGYAATLKNR
                     NLTEEEQKAAVKDFALKKGILIAVLSGVMSACFNFGLTAGQPLKDAAINLGANPLFAK
                     NPVIMFVTLGGFFTNLIYCLFLNIRNKTFRDYLSVPSTVLLRNIAFSAAGGTLWYMQF
                     FFMGMGESMLPPSMVAFAWSILMSMNIAFSNIWGIILKEWKGSDRKTIAFLVTGIFVL
                     VLSTFVIKL"
     gene            9779..10315
                     /locus_tag="PLA17_06195"
     CDS             9779..10315
                     /locus_tag="PLA17_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPK85041.1"
                     /translation="MNWIDFVITAILFYGLLQGFIDGLIIELAQLAALVLGIWGAIHF
                     SWWTADKLAVWFDMNSTWTGLISFAITFIAIVAVVYMTGKVLDSIVKTISLGFINKAL
                     GALFGVFKVTLILSVIFVFLNTINEKKRFLPSNTINNSFFYNSIADLVPSIFPVMEGG
                     NLIDSFNRHKKEKDDVTI"
     gene            10360..>11208
                     /gene="tyrS"
                     /locus_tag="PLA17_06200"
     CDS             10360..>11208
                     /gene="tyrS"
                     /locus_tag="PLA17_06200"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPK85042.1"
                     /translation="MNFVEELRWRGMIHDIMPGTEEFLLKGKTSAYVGFDPTADSLHI
                     GHLVGVMMLRHFQNAGHTPIVLIGGATGMIGDPSGKSEERNLLDEQTLRHNQECMKKQ
                     FEKFLDFNSAAPNRAVMVNNYDWMKEYSFLGFIRDIGKHITVNYMMAKDSVKKRLDPE
                     SGQGMSFTEFSYQLVQGTDYLYLYTKHGCQLQMGGSDQWGNIVTGTELIRRKAGGEAY
                     AMTCPLITKSDGQKFGKTETGNVWLDPAKTTPYQFYQFWLNVADADAEKFIRIFTMLP
                     QSEITGL"
BASE COUNT         2551 a         2966 c         2735 g         2956 t
ORIGIN      
        1 cccgtgcgca agctgcaccg accccccctg atacactgtt atctttcgag caataccggt
       61 tttccgaagc ctaccttttc aagctgcttc atctgtgtgg cagcgtcacc cacaaccacg
      121 tagtacatcc tgccgggatc aaagtactta tctgtgatgg ccttgtggtc ttcaacggtc
      181 atgttcctga tcacatcctc ctccttcctg acccagtcat cgctgaaccc gtatttcccg
      241 atgtaggtaa gcatggcagc cattgcgtca ttcgtttcaa aacggcgcgc atttgatctg
      301 atcatgctgt tctttatgaa ctgaagctcc tcctccgata tcccttcacg gtatttcgca
      361 agctcaccgg tgattatctc cagggtctca agggtggcat ctgacctgac gctggttgat
      421 atgatgaaag gtgccgggat tttcatctcg cggaagctgc tgcgtgcccc atatgtgaag
      481 cctttctgtt cgcgcagtat ctgattcagg atacttgtga aagcgcctcc catcctgtag
      541 ttggcaaaat tggcctttac ccagtcaggg tcattcctgg tcattgcaat atagccggca
      601 tagacaaccg actggcgtga gccgggaata tcgaagaaat atatctgtga ttcttccgga
      661 gcggccggta actcatagct cctggcgggc acctctctgg cctgccactc agcctcaagg
      721 ggcttcaggg cattaagcac ctctttcctt gacacgttgc ctgcaacata aatcgaagta
      781 accgacgggg agaagttagt ttcatagaaa ctcttcagaa tctcaggagt gatctgctct
      841 actgattctt ttgtgcccct gaggtcatat cccatgatat gctccgggcc atagacaagc
      901 ttcatgaact gttccgaggc aacactggcc gggcgggcct ccgcctgtat cagcccgttg
      961 cgggtgcggg tctgagccat gatgaattca gccgtgtccc agcgtggttc aagcagtatc
     1021 tccttcatga gcgaaactgt agcggcaaag ttgcgcgaca gggtgctgcc gctgagggtg
     1081 atctcttcgc cggaagcgcg catgacaacg gatgatccga gcagttcgat ctcctcctca
     1141 agctcttcgg gcgtcctgtt cctggttccc tgcggcagca ctgccgcaac catcgaggct
     1201 actcccggca ttgaaatatc atcgccatga gctccgccgg caatgaccac cgacatgctt
     1261 acaagcggca gttccctgtt ttcggtgccg taaaccctga tgccgttctt cagggtagcc
     1321 ctccagacct ccggcaccct tacttcgggc tcgggccctg cagcaggctc aatggtgcgg
     1381 tcgatagccg aagctgtctt ttccacaggt gtttcatcca tttcttctat tgccacctgg
     1441 ctggcttttg ttatgtcttc ctcgctgact gccgccctga ccgaattctc agccatcatc
     1501 tcctgctgtc ccttcgggac gaagctggtg acaatatgcg gcttaccctt gatgtaagct
     1561 tcatacaccc tcattatatc gtcacgggtg accgccctta tgttttcaat atcctttgcg
     1621 aagaatcccg gatcattcag aatggtattg taggaagcca gccggattgc cttgttcatt
     1681 acactggtca gtccctggta aaaactcttt tccatggtag ccttgatacg ctccagttcc
     1741 ctgtcggtga aaccgtcagc ctcgaagcgg gcaaacgctt cattgatggc ctcttctatc
     1801 tctttaaggc tctttccctc atttgcagta acagaaatag tgaattcccc tgccagctcc
     1861 tcggcgctgt tgtaggcaga tacccgggag gtaagctgct tttccttcac cagaaccttg
     1921 tacatagggg cttttttgcc tgatgaaagc agttctgaaa ggaagctcag ggcgtaggaa
     1981 tcctttgtat aattttcggg aaccggccat accatgttga tctccggcac attggcaaaa
     2041 ttatcctcat gatagagcct ttttgtctct gtgagagtga caggcatggc tgttctggct
     2101 gtcacctcac cgtgtgactt gatttccccg aagtacttgt caagcaggag cttcacagaa
     2161 tcagcctcga agtcacctgc aatcacaagt gttgcattgt tcggaccgta gaagttctga
     2221 tagaactctc tgacgtcatc aacgccggca ttggcaaggt cttccatctc acctattacc
     2281 tcccagctgt aaggatgcga ttccgggtag aggtttttgg atattacgta gttggtaaaa
     2341 ccataagggg agttgtcttc tccctgcctc ttttcattct gcaccacatt ctgctggata
     2401 gccagattgc ggggtgtcac tgaattgatg aagaagccca tcctgtctga ctcgagccag
     2461 agtatttttt caagcgcatt tttcggaaaa ctttcgaaat aagcggtgac atcacggctt
     2521 gtaaaaccgt tgttcgaacc cccggcattt tctatgacca tgtcaaacac cccgttggga
     2581 acattctccg agccgctgaa aagcaggtgc tcaaagagat gtgcaaagcc tgtacggccc
     2641 ggagtctccc tgctggagcc cacatgatac atgatggcaa aggaaatgac cgggtcagaa
     2701 tggtcctgat gaagtaccac ctgtaatccg ttcggcattg tgtacttctc atagctgaga
     2761 ttgagcttgt cttcctggcc ggttttgccg caagccgcca tgaatatcgc aacgattgtc
     2821 agcagtgtga cattgaacag tatacgttta atgttcatga taatagtatt tataagttat
     2881 ctgtttcaat agtttggtat tcttataaat gaatgcttat aactgcctga ttatcaggat
     2941 cggtcaactt tcccggggca ggcgctccaa ttaattgatt atgtacctgt cagtaaaaac
     3001 atattgttct gttacatttt atagaaatca tctgttcctc ctgaaggagt attttcctga
     3061 accgactgtc atgatcacgt agccatcctc attgccctca accttaatct cctttttctg
     3121 tgcaagggga aggcctccct catatatctc agcgggatct tcggcgggga tgcaaacagt
     3181 tgcagtagtg ttggcaggaa taaccacatc catttcgaaa cactcattat taatcctcca
     3241 tgtgcagcca agcagtccgt aatatgtctc aagacttgcc gacacagatg tcagtccgcc
     3301 gccgatgtgc ggttttatga tgatcttttt atatccgggc ccctcttccc tggtgtccac
     3361 tcctgcaatt acacggtaca tccagtcgcc gatggcgccg tatgcatagt ggttaaacga
     3421 gttcattccg ggagtctgga aagagccgtc aggcttctgg ccgtcccatc tctcccagat
     3481 ggttgtagct cccattgtta cgggataaag ccatgaggga tatttctttc tcagcaggag
     3541 ttcataggcc aggtcagtat aaccgaagcg cgacagcaca tggcagaggt aaggggtccc
     3601 caggaagcct gtggtaaggt gtccgtagct tttgacgttt tccgccaggc gtctggcagc
     3661 ctgctccctg aggtcaaccg gcagcatatc aaaattcagt gccaggacgt atgcggtctg
     3721 tgtaccggag acaaggcgtc cgttaggagt cacatattcc ttcatgaatg catccctgac
     3781 ccgttcgagc agtttaccgt atgagtcggc atcatctctc tttccaagca cgtttgcggc
     3841 ctttatcagc aactgtacag agttggcata gaagcactga gctatcaggt atttgtcagt
     3901 cactgcagcc atcccgctgt tgtcatcgtc aggcctgtag aagagccagt cgccatagtg
     3961 gaagccggag ttccacaggt catcccttgc cgtgctcctg atactttcga cgtaggcttt
     4021 catcgagcca tactgctgct ccagcagttg ccggtcgcca tagaccaggt acatgttcca
     4081 gggagctatt gttgccacat cggcccagcc ggtactgtta accgatccgg ctcccaggat
     4141 atttggcacc acatggggca ctttgccgtc aacctggtct gcttccaggt ctttcagcca
     4201 tttggcaaag aaattattga cattcatatt gaaagctgca gttcgcgaga atgcctgtgc
     4261 atcaccggtc catccgagtc tctcgtcgcg ctgcggacag tcggtgggta tatccaggaa
     4321 gttgcccttc tgtccccact gaatattatg ttgtaacctg tttatcaggc tgtcggaggt
     4381 gacgaatgac cctgtcggcc tcattgccga atagagggca accgcggtga agtctccggg
     4441 cgtgagcttg ccggggtatc cttcaacctt caggtacctg aatccgtgcc aggtaaaacg
     4501 tggctcccaa aactcctccc cgccgccctt caggacataa atatcacgtg cttttgctga
     4561 ccgaagatta gcggtataga agttaccatt gcggtcaagc acttcggcat gtgagaggat
     4621 tacactgtct ccggcgtttc ctcttaccct catctgaacc cacccgacaa ggttttgtcc
     4681 gaagtcgata acctggtcac cggccggggt ctccatgatc tgaacgggaa caaaggtttc
     4741 gtgcttcttc acaggctcat tttgtgtagc tatcagcaca tccatgctat gcgctgcttc
     4801 tttcaccccg tcccagccag tgtcatcata tccggggagc gtccagccct ttttctccat
     4861 cctggcgtca tagatttcac cgtgatagat ctctgagtag cggacaggac cggtggatga
     4921 tttccaggag ccgtcagaga ttatactttc agtggagccg tctgacaggg tgatatcaat
     4981 ctggcataac agggcgatgt cgttgccata gaaattgaag ctgcttctga accctatgtt
     5041 gccacggtac catccgctgc cgagcatcac accgatggcg ttgctgccgt ttttcagcag
     5101 gtgggtcacg tcatatgcct ggtattgcag ccgtttgccg taggaggtcc agcccggggt
     5161 aaggaaggca tcgccgatgc gttttccgtt gagttccgcc tcgtataaac cgtgtgctgt
     5221 tatataggcc gtagccctga caatcttccc gctgacggta aactcttttc tcatcatcgg
     5281 accgggtctc atcaccgtat cttcggcata gcccggttcg atccattttg ccttccacca
     5341 gttcttgccg atgatgccgg ttctgaattt tgccggcggg ctccatgccg accttttccc
     5401 tccgttatcc caaaccctga cctgccagtt gtaatccctg cctgatgcaa gaggctctcc
     5461 tgcatagacg atatgaactg actggtctga atttaccctg ccagtattcc ataccggccg
     5521 cgtgccttcg gagaccctga gttcataagc tgtctgtacc gtattccttt tgtaagagac
     5581 aagctgccac gagaagcgcg gttgagcagc atcaatgccc acggggttag tcatgttttc
     5641 agtgagcaat ccggttacgg aaacctgtgc cggcagagtc aggcaaaggg taaccagtgc
     5701 aagtgaaaga acaattttct ttgtcatatc tcagtcatta tcgataattg cctttagtcc
     5761 caggtagaca ggcggaagac ttttgccaac ggcactttcg tatattgtca ttgcagcgcc
     5821 gagtgcagtg gagttttcaa cctggctggc ataaaccctt ttgtcaggaa gccgggcagc
     5881 cagaaaccgc acgaaggtgt cattccgtgc aaaaccgcct gtgacgtaca ctatctcggt
     5941 cttgtcgtcg gcgggtataa taagtctcca tgattccatg cagagatcaa ccaggtcaca
     6001 tatcatctgg tgataggcat cggcgtatgt aaggtaatga gagaggtcgg cctgctcgtc
     6061 aacgtatccg ggcggtatcc cctgacggaa aaagacccgc ccatttcctg aggcaagaag
     6121 cttctctatc ttttggcttt tgatctttat ggttttgtac agctcgtcgg tgaccccgaa
     6181 gtggtcatcg agcctggcga cattcatgtc atggatatgc cccaggaaaa cgcgtgatgc
     6241 ttttacctgc tgactgtcaa tgctcaggta gcagagggtg tcattgcgta gctggtcagc
     6301 tgtcaggggc tccgtgttga aaggattcat gaatatgcac caggtacctg tggagacaag
     6361 gataaagggt ctgtcactgc ttttcagata gggcaccagc gaggcggagc tgtcatgcag
     6421 accgataccg gttctgaccg tccggccttc aatgtttaca tcagcaacac cggatggtac
     6481 caccggatca ggaagcttta ttttttcagc cttcagccag gcatggtact gcattttttc
     6541 aaaatcccac atggccgtat ggcaaccgac agatgtatat tctgacgcct ccctgcctgt
     6601 aaatatacgg ctgaggtatt gtggcagatg gagtgtggtt ctgatacgtg cgaagagagc
     6661 cggcttcatc tttttcagcc agaggagctg aagccccgag ttgagcatcc caagagccgg
     6721 tgaagccgta atccggctga actcctccac tccgccccag gcttcataga actcgtccgt
     6781 cagcccttca ggcatgggtt tcaggtaatt gtagagaggg gtgacccttt cgccctgatg
     6841 atcaaggtgc ataaggctgg caccataaga ggagaagttc aatgcaatga cctgataatc
     6901 ttctgaccgg acggcttctg caagccgtga tttcatccat gagacaatcc cttcgatgtc
     6961 atcgctttcg aaaccatcgt cgtcacgtat ttccccgaat accttctctt cctgatgaat
     7021 aaggataagg ttcaggtcga acaacaggaa cttcttgttg gtcttcccta tatcgaaaac
     7081 tgcaataaca tccatgatta ttcaggtacg ttcagagagc acttctttct catacttttg
     7141 tatttcaaca atataatctt ccgccacggg aacattgcct gtcaggcacc aatagtcgta
     7201 gaccgccccg agcggttttg tcttcatcac ctccatcagc gccagccttt caaagtacct
     7261 gccctcctcc tcatatttcc tgagtgtctc agtcggttcg agcagtgcat ggaggaatgc
     7321 cagctgtgca gcccgggtgc cgacaacata ggcgccgatg cggtttatgc tagcatcgaa
     7381 gtaatcgagt cccacatgca ctctttccag cgctttgcag cggacaatct cctgggcgat
     7441 aagctgaatc tcatcgttga atattaccac atgatcacta tcccaccgca ccccgcgtgt
     7501 cagatgaagc atcagttcat cgacatagag caggagggag gagattttgt ctcctatctg
     7561 ctcggtcggg tgatagtgac cgttgtcaag agtaatcagc ttgttgtttt ttattgcgta
     7621 gcccatataa aactcggcgg agccaaccac catcgattcc gatcctattc cgaaaagctt
     7681 gctttcgagt gcatccctga ggtattcagc ggggaattcc tcactgaaga tttcgtcaag
     7741 tgactccttc agcagggcac ggagtgcata gcggtcaacc ggagtgtcct ttgagccgtc
     7801 aggtatccag aggttgttga cgcacggagt tcccagctgt ctgcccattt cggcagcgat
     7861 tctcctgcac cgtttcgcgt gttcaatcca gaagcggcgg ttcttttcat tcctgccgga
     7921 gagggtaaag ccatcttccg ccagggggtg tgagaagaag gtgggattga agtccagacc
     7981 tgtccccagg ttttttgccc aatctatcca tccctggaag tggtcggtgg tgatgctgtc
     8041 acgatcaatc ttcgttccgc cgaactcacc atacatggcg tgaaggctca ctctctggct
     8101 tccgggcaga agactcatca ctttctccat atcctgcctc agctggctga cactgtttgc
     8161 tttgccggga taattgcctg ttgcctgtat tcctccaccc gaaagggttg catcgggtgt
     8221 ctcgaacccc ccgacatcat cagtctgcca gcaatgaaga cttatactga tgctgtcaag
     8281 cctgcgcaaa gcctcatctg tgtcaacccc aagggccgca tactgttcgc gggcatcttg
     8341 gtatgcctgc agtaccttct cttttttcat ctccggtgat atattaatct tattacaatt
     8401 taatcacaaa tgtcgagaga acaagtacga aaatccctgt taccaggaaa gcaatcgtct
     8461 tcctgtcaga tcccttccac tctttcagga tgattcccca tatattgctg aatgctatgt
     8521 tcattgacat gagaatactc caagcgaacg ccaccatcga tggcgggagc atgctttctc
     8581 ccatgcccat aaagaaaaac tgcatgtacc agagtgtacc tcctgccgct gaaaaagcga
     8641 tatttctcaa cagtaccgtg gaaggcactg acagatagtc tctgaaggtt ttgttcctta
     8701 tgttcaggaa gaggcaatag atcaggttgg taaagaaacc tccgagggtg acgaacatta
     8761 tcaccgggtt tttggcaaag agggggtttg ctccaaggtt gatggcagca tccttgagag
     8821 gctgtcccgc cgtcaggccg aagttgaagc atgcgctcat gaccccggaa aggacagcaa
     8881 tcagtatacc ctttttcagg gcgaaatcct tcacggcagc tttctgctcc tcttctgtca
     8941 ggttcctgtt cttgagggtt gcggcataac cgaggatggc tataccggca agactgacgg
     9001 aaacgcctgt gagcatcagt atccctgacc ggtctgcgaa gaggttctgc ccgcttatca
     9061 ccggtgggat aagcgttcct atcgctgcgc agaggcccag tgccactgac tgtccgaggc
     9121 ccactcccag gtacctcatg ctcagcccga aggtcagccc gcctataccc cacagtgcac
     9181 cgtagaagac agcccaccat ttggaggagg ccggggcctg gctgatgata gctccgagag
     9241 tacctgcagg aacagtgatc catgcaaaca ggagagggac gattatccat gagaacagac
     9301 cctgcacgat ccagtatgat tcccatgacc agcccctgac ctttcttatc ggtacgtaaa
     9361 agctggcagc acacatgctg cctatgctca tgataatgat gctcaggatg ggattcgggt
     9421 tcataaggtg gaaagattgg cctgtcaaat atagtgattt gccggttctg aactggctca
     9481 gcgatttacc ggatattcat ttatttcatt cttgatatta tcagttactg tctgtttact
     9541 tatcctgaca gacattaata gtgactgccg gtttccggcc gttcacttat cctggcagac
     9601 attaatggtg actgccggtt tccgtccgtt cacttatcct ggcagccacc aatgcttact
     9661 gccggctgct tgtcgttcac ctatcctgac aaccataaag ttgactgtca attaccggcc
     9721 ggggtattgt caattgcctc tcattttctt aacttcatcc gctcaaacac accaaacaat
     9781 gaactggatc gactttgtta taactgccat tctcttttat ggccttttgc agggattcat
     9841 tgacggtctg ataattgagt tagcccagct ggcagcgctg gtccttggga tttggggcgc
     9901 catccacttt tcatggtgga cggcagacaa gctggccgta tggtttgaca tgaactcaac
     9961 atggaccggc ctgatctcct tcgcaatcac attcatcgcg attgtcgctg ttgtctacat
    10021 gacagggaag gttcttgact cgatagtgaa gactatttcg ctgggcttca tcaacaaggc
    10081 acttggcgct cttttcggtg tctttaaggt cactctcata ctcagtgtaa tttttgtctt
    10141 cctcaatacc ataaacgaga agaagcgttt ccttccgtca aatactataa acaactcatt
    10201 cttctataat tccattgccg acctggtacc atcgattttc ccggtgatgg aggggggcaa
    10261 cctgattgac agcttcaacc gccacaaaaa ggaaaaggat gatgtcacca tctgagcggt
    10321 tttgatatct ttgcgacacc taaatccaaa acgctgatga tgaactttgt tgaagagctg
    10381 aggtggaggg ggatgataca tgacatcatg cccggcacgg aggagttcct gttaaagggg
    10441 aagacctcgg cttacgttgg ctttgaccct acagctgatt cattgcatat tggccatctg
    10501 gtaggagtga tgatgctcag gcattttcag aatgccgggc acaccccgat tgtgctgatc
    10561 ggcggcgcaa caggtatgat tggtgaccct tccggcaagt cagaggagcg taatctgctt
    10621 gatgagcaga cactgcgcca taaccaggag tgcatgaaga aacagtttga gaaattcctc
    10681 gacttcaatt ccgccgcacc gaacagggcg gtaatggtca acaactatga ctggatgaag
    10741 gaatactcgt ttctcggctt catcagggat attggcaagc acatcactgt caactacatg
    10801 atggccaagg attccgtaaa aaagaggctt gacccggaaa gcggccaggg aatgtcattc
    10861 accgagtttt cataccagct ggttcaggga actgattacc tttatctcta caccaagcat
    10921 ggatgccagt tgcagatggg cggatcagac cagtggggaa acatagtcac cggcactgaa
    10981 ctgatcaggc gcaaggcggg aggagaggct tatgccatga cctgcccgct gatcaccaag
    11041 agtgacggcc agaagttcgg caagacggaa acaggcaatg tatggcttga cccggcaaaa
    11101 accacacctt accagttcta ccagttctgg ctcaacgtgg ctgacgccga cgcagaaaaa
    11161 ttcatcagga tattcacaat gttaccacag tcagaaataa ccggactg
//
LOCUS       DAOPBK010000082        11206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5697_length_11206_cov_4.742086, whole genome shotgun sequence.
ACCESSION   DAOPBK010000082 DAOPBK010000000
VERSION     DAOPBK010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5697_length_11206_cov_4.742086"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..951)
                     /locus_tag="PLA17_06205"
     CDS             complement(130..951)
                     /locus_tag="PLA17_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HPK85043.1"
                     /translation="MFSDRLQNFDLSLASSSPRRRQLMEEAGFRFRVVAIRFKETQPS
                     HLRGKEIAEFLAAGKSASWDKPLAPGQVLITADTIVWSHDENLGKPADDAEAIRFLRK
                     LSGCRHEVITGICLRDSKKEMILSVTTGVTFRELSDDEIEYYVSNYRPLDKAGAYGIQ
                     EWIGLRGITTIEGSYYNVVGMPVSELYTALLHFTGNEIGHKTGYETASETGKESGHEP
                     GSETGYEPGKETSSKTVKESGHEPAREIRHETGHKSGNATGNETRNKTRNKTRNI"
     gene            complement(953..1963)
                     /locus_tag="PLA17_06210"
     CDS             complement(953..1963)
                     /locus_tag="PLA17_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HPK85044.1"
                     /translation="MKHSRGERLNDLLNLVRLPNLLVVAMTMMLMRYAIIRPLLGAVQ
                     VTMAGDPGTLVAMDFRLSWFDFLILVISTCLITAAGYVINDYFDIRADLINRGTIIVG
                     NTITRRMAMMYHNVFNLLGVIGGIYVSARIGYVWLGIFFILISGLLYFYSATYKRQFL
                     IGNIIVALLVAMVPMLVVVYDAAPVYVYYSQASTGFPGVAILFWWVGGFALFAFLTTL
                     IREIIKDMEDCEGDRETGRKTLPVISGLTVSKVIVVILSVITVALLYYVWYRHLHDTV
                     TLVYISILLAVPLVAVIVRVLTSSEKRHLTLASRLMKLIMLAGILYSLVAGAIISSGK
                     IL"
     gene            complement(1944..2465)
                     /locus_tag="PLA17_06215"
     CDS             complement(1944..2465)
                     /locus_tag="PLA17_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPK85045.1"
                     /translation="MTNFKEELRGVKAFVFDIDGVLSKQTIALSVRGMPLRTVNLRDG
                     YAIQLAVKKGYHVGVISGANSREYITRLKALGVNDIFLNSRIKKESMKELAERWNVEL
                     KSVLYMGDDIPDFEVMKMVGLPACPADADSEIKQIAVYISDKNGGEGCVRDVIEQALR
                     LHKNWMDYEAFSW"
     gene            complement(2462..3244)
                     /locus_tag="PLA17_06220"
     CDS             complement(2462..3244)
                     /locus_tag="PLA17_06220"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent NADP oxidoreductase"
                     /protein_id="HPK85046.1"
                     /translation="MSVYKISFIGAGNVAESLATGLAAAGHRIVSVASRNGISARELA
                     QKTGAGYRHDLTVPDECDLLLICVTDRSVAEVAASVITHPDTVIAHTAGSIPLEALGR
                     TTGAGVFYPLQTFTKAFPPDLRKVPFFIEATDSKAIEVLRNTGESIGAGVWECDSESR
                     KKLHLAAVFTNNFSNFMMTVGEAVAREAGIDPVLLRPLMEETARKALRSGPEAAQTGP
                     AVRHDTGTVKSHIELLSFSPQYQKLYRLVSRMIAGHYRKRKK"
     gene            complement(3279..3677)
                     /locus_tag="PLA17_06225"
     CDS             complement(3279..3677)
                     /locus_tag="PLA17_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85047.1"
                     /translation="MKRSRILATVGLVLAVAALVAMTSGCKKQLKCGCDGDAIDSLKL
                     ENVYITYDAVNKTAYFTRVWNPYGTYYFCNPSKWIDELTKYNNGEEILLSGEFFYDCS
                     YVMYSSNSYYNYPPMYQVMVTAVAPQEFGK"
     gene            complement(3783..4100)
                     /locus_tag="PLA17_06230"
     CDS             complement(3783..4100)
                     /locus_tag="PLA17_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPK85048.1"
                     /translation="MSDIKNQSQFNIELGEDVAGGIYSNLAIITHSPAEFVIDFVRIM
                     PGVPKPKVRSRIILTPEHAKRLVAALNDNIAKYEAVNGPIREVKGSGPVMPVTFGGPT
                     AQA"
     gene            complement(4113..8405)
                     /gene="rpoC"
                     /locus_tag="PLA17_06235"
     CDS             complement(4113..8405)
                     /gene="rpoC"
                     /locus_tag="PLA17_06235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPK85049.1"
                     /translation="MSFRRDNKQKTSYSKISIGLASPEEILDKSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIS
                     LVVPVAHIWYFRSLPNKIGYLLGLPTKKLDSIIYYERYVVINPGVKAADGVKYLDFLS
                     EEEYMEIVETLPKENQYLDDDHPDKFVADMGAEALYKLLARLDLDELSYSLRHRANTE
                     TSQQRKSEALKRLQVVEAFRESRNVNRPEWMIVKIVPVIPPELRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAIDSLFDNSRKANAVKTDANR
                     PLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKLHETGIPKDMAAELYKPF
                     IIRKLIERGIVKTVKSAKKIVDRKDPVVWDILENVLKGHPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQLHPLVCTAFNADFDGDQMAVHLPLGNAAILEAQMLMLASHNILNPANG
                     APITVPSQDMVLGLYYITKARKSTETIKVKGEGTVFYSPEEVRIAYNENKVDLHAMIR
                     VKVNDIVDGQNVNHLIDTTVGRVLFNEYVPGEVGFINELLTKKSLRDIIGTVLKKSGT
                     AKTAEFLDAIKDLGFRMAFIGGLSFNLDDVIIPKEKELFVQEGYEQVEEVLANYSMGF
                     ITNNERYNQIIDIWTHVNARLTQSLMKQLSNDKQGFNSVYMMLDSGARGSKEQIRQLS
                     GMRGLMAKPQKSGASGSEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADA
                     GYLTRRLVDVSQDVIITEEDCGTLRGLVATAIKNNEDIVESLYERILGRTTVHNIYHP
                     LTGELIISSGEEITEEVADKIDQSPIEQVEIRSVLTCESKKGVCAKCYGRNLATGRMV
                     QKGEAVGVIAAQSIGEPGTQLTLRTFHVGGTASNITTESSLIARYGGIAMIDELRTVA
                     KKDPEKGEYNIVIGRLAELRIVDKTTGIALTTHTIPYGSRLYIKPNQEVEKGQLICEW
                     DPFNAVIISEVSGKVSFDYLIEGVTYREESDEQTGFKEKVIIESRDKTKNPTIKIMTS
                     EGVEIKSYNLPVGAHLVTDLNKLVEAGDTIVKIPRAVGKSGDITGGLPRVTELFEARN
                     PSNPAIVTEIDGEVTYGKIKRGNREITITSKSGEMKKYLVPLSKQILVQENDYVRAGT
                     SLSDGAITPADILAIKGPTKVQEYIVNEIQEVYRLQGVKINDKHFETIVRQMMRKVEI
                     IDPGDTKFLERQIVDKLEFMDENDWVYGKKIVLEPGDSQTLKPGMVITARKLRDENSL
                     LKRRDLKLIESRDAVPATSQQVLQGITRAALQTSSFFSAASFQETTKVLNEAAILGKV
                     DYLEGLKENVIVGHLIPAGTGLREYNNMIVGSLDDYESLVRAGQSPVEVEQD"
     gene            complement(8447..>11206)
                     /gene="rpoB"
                     /locus_tag="PLA17_06240"
     CDS             complement(8447..>11206)
                     /gene="rpoB"
                     /locus_tag="PLA17_06240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPK85050.1"
                     /translation="ALLKEAEGLDEAAIKALVRSRVHDLIPRHIIPDDIVASVSHLVG
                     MPYGIGHTDDIDHLGNRRIRSVGELLQNQIRVGLTRLERVVRERMGLQDMDVAMPSNL
                     INIRPVTAAIKEFFGSSQLSQFMDQTNPLAEITHKRRLSALGPGGLSRERAGFEVRDV
                     HYTHYGRLCPIETPEGPNIGLISSLCVYARINELGFIETPYRDVHDGRVNLNNDDVKW
                     LTAEDEEHLTVAQANAPVNDDGTFVNPRAKSRLGADYPFEEISKVQMMDVAPNQIASI
                     AASLIPFLEHDDANRALMGSNMMRQAVPLIRPEAPIVGTGLEEQVIKDSRILIVAEGD
                     GVVEYVDANEIVVRYSRTDEEKFVSFDDDVKRYLLPKYHKTNQNTTINLIPIVRKGEK
                     VVKGQVLTEGYGTKSGELALGRNLKVAFMPWRGYNFEDAIVISEKVVQEDIFTSVHID
                     EYLLEVRDTKRGMEELTSDIPNVSEEATKDLDENGLIRIGAHIKPGDILIGKITPKGE
                     SDPSPEEKLLRAIFGDKAGDVKDASLKAGPSLEGVVIDKKLFTRAIKDRKSKTAEKPL
                     LDKIDADFNRSVDELKARLVDKIFILVNGKTSQGVKDYLNVDIIPKGAKFTLKQLQEI
                     DYMNINPNKWTTDKKKNDSIKQLLHNYVIKYKEIDGVYKRRRYNITIGDELPAGIVRL
                     AKVYIAKKRKIRVGDKMAGRHGNKGIVAKIVRQEDMPFLADGTPVDIVLNPLGVPSRM
                     NLGQIYETVLGWAGKELGMKFSTPIFDGAQQDEINELTGKAGLPKNGTTYLYDGGTGE
                     RFDQPATVGMIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAFGAAHILQEILTIKSDDVIGRAKAYEAIVKGEPMPMPGIPESLNVLLHELRGLGL
                     SINLE"
BASE COUNT         2412 a         3061 c         2734 g         2999 t
ORIGIN      
        1 tgaagccatt ctcctgcatc agtttgatgt tgtacttctc aattagcact ggtatccaca
       61 tcccctcatc tgccaacgct gccggtgtgg ccatggcaaa cagcatcgca atagtgaaaa
      121 gtttcagttt catatgtttc tggttttgtt tctggttttg tttcttgttt cgttcccggt
      181 tgcgtttccg ctcttatgcc cggtttcgtg ccttatttct ctcgcgggtt catgcccgct
      241 ttccttaact gttttactcg aggtttcctt accgggttcg tacccggttt cgctcccggg
      301 ttcgtgcccg ctctctttac cggtttcact cgcggtttcg tacccggttt tgtgcccgat
      361 ctcgttcccg gtaaaatgaa gaagggctgt atagagctcg ctcaccggca ttcccacaac
      421 attgtaataa gacccttcta ttgttgttat gcccctgagg cctatccatt cctggatgcc
      481 atacgcaccg gccttgtcga gggggcggta gttgcttaca tagtattcaa tctcatcatc
      541 actcaattcc ctgaaggtga ctccggtagt gactgaaagg atcatttcct ttttactgtc
      601 tctcaggcat atgcctgtga tcacctcatg tctgcatcct gagagcttcc tcaggaaacg
      661 gatcgcttcc gcatcatcgg caggcttgcc caggttttca tcatgggacc agacgatggt
      721 gtcagcggtg atcagcacct gccccggagc aagcggctta tcccatgagg ccgacttgcc
      781 tgccgccagg aactcggcga tttcctttcc cctgagatga gatggctggg tttctttaaa
      841 tctgatggca accaccctga acctgaatcc ggcctcctcc atgagctgcc tgcgccttgg
      901 tgacgacgag gcaaggctaa ggtcaaagtt ctgcaatctg tctgaaaaca ctctaaagaa
      961 tttttcctga agagataatt gctcctgcaa caagtgagta tagtattcct gccagcatga
     1021 taagcttcat cagcctgctt gcaagcgtaa gatgcctctt ttcgctcgag gtgagcaccc
     1081 tgacaatcac tgccaccagc ggaaccgcca ggagaatcga gatatagaca agggtgacgg
     1141 tgtcatggag atgcctgtac cagacataat aaagcaatgc cacggttata actgacagaa
     1201 tgacaacaat gaccttgctt accgtgagcc ctgatatcac cggcagcgtt tttctgcctg
     1261 tctcacggtc accttcacaa tcttccatat cctttattat ctcgcggatg agggttgtaa
     1321 ggaatgcaaa cagggcgaat ccgccaaccc accagaaaag tatggccaca cccgggaagc
     1381 cggtggaggc ttgtgaatag tagacataaa caggtgctgc atcatacact accaccagca
     1441 ttggcaccat tgctaccagc agcgccacga taatgtttcc tataaggaac tgccgtttgt
     1501 aggtggccga atagaagtaa agcagcccgg atatcagtat aaaaaagatc cccagccata
     1561 cataacctat tcgtgcagat acgtatatcc cgcctataac gccaagtaaa ttgaacacgt
     1621 tgtgatacat cattgccatg cgcctggtaa tggtgttccc gacaatgatg gttcccctgt
     1681 tgatcaggtc agcccggata tcgaaatagt cgttgatcac atatcctgct gcagttataa
     1741 ggcaggttga gatcacaagt atgaggaagt caaaccagct gaggcggaag tccatggcaa
     1801 ccaaagttcc gggatctccg gccattgtca cctggacagc tcccaggagc ggcctgatga
     1861 tggcatatct catcagcatc attgtcatgg ctactaccag aaggtttggc agcctgacaa
     1921 gattcagaag gtcatttaat ctctcaccac gagaatgctt catagtccat ccagtttttg
     1981 tggagcctca gcgcctgctc aatcacatcg cgcacgcatc cctctcctcc atttttatct
     2041 gaaatgtaaa cggctatctg ctttatttca ctgtcggcat cggccggaca ggccggaagg
     2101 ccaaccattt tcatcacctc aaagtcaggg atgtcgtcgc ccatgtagag gacactcttc
     2161 agctccacat tccaccgttc tgccagctcc ttcatgctct ccttcttgat gcgcgagttc
     2221 aggaatatat cattgacgcc cagcgcctta agtcttgtaa tatactccct tgaattggct
     2281 ccggagatca cccccacgtg ataccccttc ttgacagcca gctggattgc atatccgtct
     2341 ctgagattta cggttctgag gggcatgccc cgtaccgaaa gagcgatagt ctgtttagac
     2401 agcaccccgt cgatatcaaa gacaaaggct tttactcccc tgagctcttc cttgaaattc
     2461 gtcatttttt tcttttcctg taatgtccgg caatcatcct gctgacaagc cggtagagtt
     2521 tctggtattg tggtgagaaa gatagcaatt cgatatgact tttcactgtg cctgtgtcat
     2581 gtcgcacagc aggcccggtc tgtgcggctt cagggcctga acggagagcc ttgcgcgccg
     2641 tctcctccat caggggcctg agcagtaccg ggtcaatgcc ggcttccctg gcaactgcct
     2701 caccgactgt catcataaag tttgagaagt tgtttgtaaa gacagcagcc aggtgaagct
     2761 tttttctgct ctccgagtca cactcccaca ccccggcccc gatgctctca cctgtgttcc
     2821 tgagcacctc aatggccttg ctgtcagtgg cctcaatgaa gaacggcact ttgcgcaaat
     2881 caggaggaaa ggctttggta aaggtctgca acggataaaa tacacccgca ccggtcgtcc
     2941 gccccagggc ttcgagagga atactgcctg ctgtgtgggc aatgactgta tcagggtgtg
     3001 tgattactga ggcagcaact tcggcaactg acctgtcggt gacacagatc agaaggagat
     3061 cacattcgtc cggaacagta agatcatgcc ggtatccggc tcctgttttt tgagccagct
     3121 cacgggcgct tattccgttc cgtgaggcaa cggatactat tctgtgacca gcagctgcca
     3181 gtcctgtggc aagtgactca gccacattac ccgctccaat gaaggatatt ttgtagacag
     3241 acaccgttgc tggcaaatca ttgcttttat tgacagcttc actttccgaa ttcttgggga
     3301 gctaccgccg taaccatcac ctggtacatc ggcggatagt tataatatga gttactgctg
     3361 tacatcacgt agctgcaatc ataaaaaaac tcccctgaca acagtatctc ctctccatta
     3421 ttgtatttgg tcaactcatc aatccatttc gagggattac agaaataata tgtcccatac
     3481 ggattccaga cccttgtgaa atatgcagtc ttgttgactg catcatatgt tatataaacg
     3541 ttttccagtt tcagggaatc aatggcatca ccgtcacagc cgcactttag ttgtttcttg
     3601 cagcccgatg tcattgcaac cagtgcggca actgccaaaa ccaggcccac tgtggccaat
     3661 atcctgcttc ttttcatgat tctgagctgt ttagacgtta caaaataatg ctaatttttc
     3721 cttcctgcaa acaaataagc cggttatttt cacaaccggc ttaagttcag tatgttcatg
     3781 catcatgcct gtgctgtcgg tccgccaaaa gtaaccggca tgacagggcc tgatcccttg
     3841 acttcgcgta tcgggccgtt aacagcctca tactttgcaa tattgtcatt cagtgcagcc
     3901 acaagccgct tggcatgttc aggagtcagg atgattctcg acctgacctt gggcttgggc
     3961 acacccggca tgatccggac gaaatcaatt acaaactccg ccggagagtg ggttatgatt
     4021 gccaggttgg aatagatacc cccggcaaca tcctctccga gttcaatgtt aaactggctc
     4081 tgattcttga tgtctgacat aatcccctgg ttttaatcct gttccacttc gacgggactc
     4141 tgcccggcgc gtacgagtga ttcataatca tcgagagaac ctacaatcat gttgttgtac
     4201 tccctgaggc ccgttccggc cgggatgagg tgtccgacga tgacattctc cttaagaccc
     4261 tcaagatagt ctaccttccc gaggatggca gcctcattga ggaccttggt agtctcctgg
     4321 aatgatgctg cagagaagaa gctgcttgtc tggagtgcag cacgggtaat accctgaagc
     4381 acctgctgtg atgtagcggg tactgcatcc cttgattcaa tcagcttgag gtcacggcgc
     4441 ttgagcagtg agttttcatc cctgagcttg cgggctgtga ttaccatgcc cggcttcagt
     4501 gtctggctgt cgcccggttc caggacaatc ttctttccat atacccagtc attctcatcc
     4561 atgaactcca gcttgtcaac aatctgccgc tcaaggaact tggtgtcacc cgggtcaatt
     4621 atctcaacct tacgcatcat ctgcctgacg atagtctcaa agtgcttgtc attgatcttc
     4681 acaccctgga gacggtagac ctcctgtatc tcattgacaa tatactcctg gacctttgtg
     4741 ggccccttga tggcaaggat gtctgcaggt gtaattgcac cgtcagagag tgatgttcct
     4801 gcccttacat agtcattctc ctgcacaagg atctgttttg acagcggcac aaggtacttc
     4861 ttcatctcac ctgacttgga tgtgatggtt atctccctgt tacccctctt gatctttccg
     4921 taggtaacct caccgtcaat ctcggtcacg atagccgggt tggaggggtt ccgtgcctca
     4981 aacagctcgg taaccctggg cagacctccg gtgatgtcac cggactttcc tactgccctc
     5041 ggtatcttga caatagtatc accggcttca accagtttgt tgaggtcggt aaccaggtgg
     5101 gctcccaccg gcaggttgta tgatttgatc tcaactccct cggaagtcat gatcttgatg
     5161 gtcgggttct tggtcttgtc cctgctctca atgatcacct tctccttgaa gcctgtctgc
     5221 tcgtctgact cctcacggta ggtcacgcct tctatgagat aatcaaatga caccttgccg
     5281 gatacttcag agataataac cgcgttgaac ggatcccatt cgcatatcag ctgacccttc
     5341 tccacctcct ggtttggctt tatatacaac ctgctgccgt aaggaatggt atgtgtagta
     5401 agcgctatac cagtggtttt gtcaacaatc ctgagttcgg caagccggcc gatgacaatg
     5461 ttgtattccc ccttctccgg atcttttttg gcaacggttc tcagttcatc aatcattgca
     5521 atgccaccat accttgcaat aaggcttgat tctgtagtga tgttcgaggc agtaccaccg
     5581 acgtggaacg tacggagggt aagctgtgtg ccgggctcac ctatactctg tgcggcaatc
     5641 acacctacag cttcaccctt ctgcaccatc cttccggtgg cgaggttacg gccataacac
     5701 ttggcacaga cacccttctt cgactcacag gtaagaaccg aacgtatctc tacctgttca
     5761 atgggcgact ggtcaatttt gtcggcaacc tcctccgtga tctcctcccc tgaggagatg
     5821 ataagctctc cggtaagcgg atggtagatg ttatgcacgg ttgtgcggcc aaggatcctt
     5881 tcatacagtg attcaactat atcctcgttg ttcttgattg ccgtggcaac cagtccgcgc
     5941 agcgttccgc agtcctcctc ggtgatgatg acatcctggg aaacgtcaac aagacggcgt
     6001 gtaagatagc ctgcatcagc agtcttgagg gctgtgtcag caagaccctt gcgggcaccg
     6061 tgggtggaga tgaaatactc aagtaccgac agcccttcct tgaagttcga aaggatcggg
     6121 ttctcgatga tctccgagcc gctggcgcct gatttctgag gcttggccat aagccccctc
     6181 attcccgaga gctgacgtat ctgctccttc gagccccttg cacccgagtc aagcatcatg
     6241 taaaccgagt tgaagccctg cttgtcattt gacaactgct tcatgaggct ctgcgtaagg
     6301 cgggcattga catgggtcca gatatcgatg atctggttgt acctttcatt gttggtgata
     6361 aagcccatgc tgtagttggc aagcacctcc tcaacctgct cgtaaccttc ctgaacgaac
     6421 aactcctttt ctttcggaat tataacgtcg tcaaggttga acgagaggcc gccgataaat
     6481 gccatcctga acccgagatc cttgattgca tcaaggaatt ctgcggtttt ggcagttcct
     6541 gacttcttca gtactgttcc gatgatgtcc ctcaatgact tcttcgtcag cagttcattg
     6601 ataaaaccaa cctccccggg cacgtactca ttgaagagaa cgcggcctac agtggtatca
     6661 atcaagtgat tgacattctg tccgtcaact atgtcattca ccttgaccct gatcattgca
     6721 tggaggtcaa ccttgttttc gttatatgca atgcgtacct cttcgggtga atagaacact
     6781 gttccttctc ccttgacctt gatggtctca gtgctcttgc gggccttggt gatatagtaa
     6841 agcccgagga ccatgtcctg tgagggcact gtaataggcg caccgttggc cgggttgagt
     6901 atgttgtgcg atgcgagcat cagcatctgt gcctcaagta ttgcggcgtt gccgaggggc
     6961 aggtgaaccg ccatctggtc accgtcgaag tcggcgttga aagccgtaca gacaagcggg
     7021 tgcagctgga ttgccttgcc ttcaatcagc ttcggctgga aggcctggat acccagcctg
     7081 tgaagtgtcg gtgcacggtt cagcaacacc gggtgtccct tgagaacgtt ttcgaggata
     7141 tcccatacaa ccgggtcctt gcggtcgacg atcttcttcg cgctctttac agtcttgaca
     7201 atgccgcgct caatgagctt gcggataata aacggcttgt agagctcggc tgccatatcc
     7261 ttcgggatac ctgtctcgtg aagcttgagc tccgggccca cgacgataac cgaacgtgcg
     7321 gagtaatcaa ccctcttgcc gagaaggttc tggcggaagc gtccctgctt gcccttgagg
     7381 ctgtcagaga gcgacttgag aggcctgttg gcgtcggtct tgacggcgtt ggccttgcgt
     7441 gagttatcaa acagagagtc aatagcctcc tggagcattc tcttttcatt gcggagaatg
     7501 acttccggcg ccttgatctc tatcagtctc ttgaggcggt tgttacggat gatgaccctt
     7561 ctgtagagat cattaagatc gcttgtcgca aaacggccgc catcaagagg aacaagaggc
     7621 ctcagttcgg gcggtatgac cggcactatc ttgacgatca tccactcggg cctgttgacg
     7681 ttccttgact ccctgaaggc ttcaacaacc tgaagtctct tgagagcctc gctcttgcgc
     7741 tgctgtgatg tctcagtgtt tgcgcgatgg cgaagcgaat atgaaagctc atcaagatcg
     7801 agcctggcga gaagcttgta gagggcttct gctcccatat ctgccacgaa cttgtcagga
     7861 tggtcatcat caagatactg gttctctttc ggcagggtct ccactatctc catgtactcc
     7921 tcttccgaga gaaaatcaag atacttgact ccgtcggcag ctttgacacc cggattgatg
     7981 actacatacc tctcatagta gataatgctg tcaagcttct tcgtcggcag gcccagcagg
     8041 taaccgatct tgttgggcag cgacctgaaa taccatatat gggctacggg aacgaccagg
     8101 gagatatgtc ccatgcgctc gcgtcttacc ttcttctccg tcacctcaac gccgcagcgg
     8161 tcacatacga tacccttgta acgtatcctc ttgtatttcc cgcaatggca ttcataatcc
     8221 tttaccggtc cgaagatcct ctcacagaag aggccgtcac gttcaggctt gtaggtacgg
     8281 tagtttattg tctcaggctt caggacctcg ccgcttgact tgtcaagaat ctcctccggc
     8341 gatgccagcc cgattgaaat cttcgaatag ctggtcttct gtttgttatc ccttctgaat
     8401 gacatattat gctttcgttt ttatcttgtg tgaatcctca gcttaattat tcaaggttga
     8461 tgctcagtcc gagcccgcgc agttcatgca gcagcacgtt cagcgactcc gggatgccgg
     8521 gcatcggcat cggttcgccc ttgactattg cttcgtaagc cttggccctt ccaatcacgt
     8581 catcagactt gatggtcagt atctcctgaa ggatatgggc tgcgccgaat gcctcgagtg
     8641 cccagacctc catctctccg aatctctgtc cgccaaactg ggccttgcct ccgaggggct
     8701 gctgggtgat gagcgaatag ggccctattg aacgggcatg catcttgtca tcaaccatgt
     8761 ggcccagctt gagcatatag atcataccca ccgttgccgg ctggtcaaac cgctcgcctg
     8821 tgccgccatc atagagatag gtggttccgt tcttcggaag gcctgccttc ccggtgagtt
     8881 cattgatctc atcctgctgc gcaccgtcaa agatgggtgt tgaaaacttc attcccagct
     8941 ccttgccggc ccatccgaga acagtctcgt agatctggcc gaggttcatt cgcgaaggca
     9001 caccaagcgg gttgagaaca atatctacag gtgtaccgtc agccaggaaa ggcatgtctt
     9061 cctgccttac tatcttggcc acaataccct tgttgccatg gcgcccggcc atcttgtcgc
     9121 caacccttat cttgcgcttc ttggcgatat ataccttggc aagcctgaca atacctgcgg
     9181 gaagctcatc accgatggta atgttgtacc tcctcctctt atagacaccg tcaatctcct
     9241 tgtacttgat cacatagttg tgaagaagct gcttaatgct gtcattcttc ttcttgtcag
     9301 tggtccactt gttgggattg atgttcatgt aatcaatctc ctggagctgc ttgagggtga
     9361 actttgcacc cttggggata atgtctacat tgagataatc ctttactccc tgtgatgtct
     9421 tgccattgac aaggataaag atcttgtcca ccagccttgc cttcagctcg tcaaccgacc
     9481 tgttgaagtc agcatcgatc ttgtcaagca gtggtttctc ggctgtcttt gacttgcggt
     9541 cttttatagc cctggtaaac agtttcttgt ctatgacaac accctcgagt gacgggccgg
     9601 ccttgaggga tgcatccttc acatcaccgg ccttgtcgcc gaatattgcc ctgaggagtt
     9661 tttcctcggg agaaggatcg gattcgcctt tgggagtgat cttgccgatg agtatgtcac
     9721 cgggtttgat atgtgctccg atgcggataa gtccgttctc atcaagatcc tttgttgcct
     9781 cctcgctgac gttgggtatg tctgaggtga gttcctccat cccgcgcttg gtgtcacgca
     9841 cttcaaggag gtattcatca atgtgaaccg aggtgaagat atcttcctgg acaaccttct
     9901 cgcttatcac gatggcatcc tcgaagttgt agcccctcca gggcatgaat gccaccttga
     9961 ggttacggcc gagagccagc tcgccgctct tggtgccata accctccgtg agcacctggc
    10021 ccttaaccac cttttctccc tttctgacga tggggatcag gttgattgtg gtgttctggt
    10081 tggtcttgtg atacttggga agcaggtaac gcttaacgtc atcgtcaaag ctgacgaact
    10141 tctcttcgtc tgtccggctg tagcggacga cgatctcgtt tgcgtcaaca tattcaacca
    10201 cgccgtcacc ttcagccact atgaggattc gtgagtcctt gatcacctgt tcttcgagtc
    10261 ctgtgcccac aatcggggct tcgggcctta tcagcggcac cgcctggcgc atcatgttcg
    10321 atcccatgag cgccctgttg gcgtcgtcat gctcaaggaa ggggatcagt gatgctgcta
    10381 tcgatgctat ctggttcggc gcaacgtcca tcatctgcac ttttgatatc tcctcgaagg
    10441 ggtagtcagc cccaagcctt gacttggccc tgggattgac aaaggtgccg tcatcattca
    10501 ccggcgcgtt ggcctgtgcc acggtgagat gctcctcatc ctccgcggta agccacttca
    10561 catcatcatt gttgaggttc accctgccgt catgtacgtc acggtaggga gtctcaatga
    10621 atccgagctc attgatgcgt gcatagacgc acagtgatga aataagtccg atgttcggtc
    10681 cttcaggagt ttcaatggga cagaggcggc cgtagtgcgt gtagtgcacg tctcggacct
    10741 cgaatcctgc acgctcacgt gacagaccgc cggggccgag agccgagaga cggcgcttgt
    10801 gggttatctc ggcaagcggg tttgtctgat ccatgaactg gctcagctgc gaggagccga
    10861 aaaactcctt gatcgccgcc gtcacggggc ggatgttgat gaggttcgac ggcatggcca
    10921 cgtccatatc ctgcaagccc atgcgctcgc gcacaacgcg ctcaaggcgc gtcaggccca
    10981 cgcggatctg gttttgcaaa agctcgccca cgctgcggat gcggcggttg ccaaggtggt
    11041 cgatgtcgtc cgtatgtcca atcccgtaag gcatgccgac gagatgggac accgaggcca
    11101 cgatgtcgtc cggtatgata tgccgcggga tgaggtcgtg tacgcgcgaa cgtacgagcg
    11161 ccttgatcgc cgcctcgtca agcccttcgg cttccttgag cagcgc
//
LOCUS       DAOPBK010000083        11169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5711_length_11169_cov_5.415062, whole genome shotgun sequence.
ACCESSION   DAOPBK010000083 DAOPBK010000000
VERSION     DAOPBK010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5711_length_11169_cov_5.415062"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="PLA17_06245"
     CDS             <1..1246
                     /locus_tag="PLA17_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85051.1"
                     /translation="DGLFDEDGPDDLDGDGNISTMRIRDTLGRLRSDPADKRLMITVK
                     PGEKGEWTLLGQEGIDNDGDGLINEDGEGYVDGNRNWGMNWKPNYVQRGAGNYPFEGK
                     GIRAIAEWMLARPNIILVFAFHNNGGMYLRGPTSKEAGELPQEDVAVYDYLGEHMEKI
                     VPGYRYLISWKDLYPTYGDFTDFTDKIIGSYSLVGELFQPATETYDGTLKRVEEERSR
                     SGDPSAGDRQRLLFDDNVNQGELYKEWTAFKHPVYGDIEIGGWTKMSSRLPHPFMLQD
                     LVHRNASSVIFAARQTPRVTMDVFEVKKIGGDLWSVRVRLVNSGAIPSVTYETIRNKL
                     YPVDKLTVTGRNAKVASGGVLTNAWMNQVNYKEYRPEVQMCQVPGMGKVEYQFLISGR
                     GDIEIRYESRKAGSVSKTVTLK"
     gene            complement(1393..2139)
                     /locus_tag="PLA17_06250"
     CDS             complement(1393..2139)
                     /locus_tag="PLA17_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HPK85052.1"
                     /translation="MKIPEHPTLNREWDKKEIKKKTDKMLEEIGELQYKMYAQGKYSI
                     IVLLQGIDASGKDGVAKGILKFCNPVGTEVYSFKKPTDLEYAHDFLWRVHNVCPAKGI
                     MKVFIRSHYEDILVPSVEKYIPADIIEKRYETINTFEKMLEDHGTKVLKFFLNVSKET
                     QEERLMERIEMEEKHWKHKDGDWDTREKFDQYLKVYQKILTRCNDVPWHVVPADKNWQ
                     KLYTVAEVVLKTLKELDLEWPALVTERFGK"
     gene            2688..2969
                     /locus_tag="PLA17_06255"
     CDS             2688..2969
                     /locus_tag="PLA17_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPK85053.1"
                     /translation="MIKVNEYFEGNVKSMALENEDGRATVGVMAAGDFEFGTSTVEYM
                     TVVSGKMTVLLPGETEWKTYVPFQTFVVPANQKFKLKMDQPAAYRCLYK"
     gene            2982..3374
                     /locus_tag="PLA17_06260"
     CDS             2982..3374
                     /locus_tag="PLA17_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPK85054.1"
                     /translation="MAGHKGDPYYDIFLRYEMWLETIGKERILDGEGFDLLVDLDRTG
                     SLTATARNLGMSYRKAWGMLNDVERHLGFRLTEKRRGGASGGRTLLSEKGQQLVMAYN
                     SLRHETDIAMKSIAKVFFNRINEINGLK"
     gene            3371..4339
                     /locus_tag="PLA17_06265"
     CDS             3371..4339
                     /locus_tag="PLA17_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK85055.1"
                     /translation="MRTVLLALAVMSVLFSGCAGGSGSGRGDNKLIIFHAGSLSVPVR
                     ELSAAFMKENPGVTILTEAAGSLHAARKITELKKPCDIMLSADHFVVSSLLVPEYASW
                     NIRFATNEIVIAYRAESKYSSIFREDNWADILLKDDVIMGRSDPDADPCGYRTVFAAK
                     LAEEYYGRPGLADSLLRKNTEYIRPKEVDLVALVSSGVIDYMFQYKSVAIQHGFGYLE
                     LPDEISLSSPLLTERYGTVSYMIPGETPGIRKEVCGDYISYSGTVLDRAPQKELAMKF
                     FEFMLGEEGAAIFRKCGQEPLVPAVADDTDAVPSSLRKFIAVVPAK"
     gene            4348..5157
                     /locus_tag="PLA17_06270"
     CDS             4348..5157
                     /locus_tag="PLA17_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK85056.1"
                     /translation="MKNHTLFNWIFTLLASLVLLFIIAPLIGMFLHTSAEEMRSTASD
                     PVVIRSVWYTLAIAFAATILFAIGAVPLAWIMARRKFPLKNIVQGIIDLPVVLPHTAA
                     GIALLGFISREGFLGGIAQKAGILLVNNGAGIALAMAFVSLPFLINAARDGFAAVPER
                     TEKAALMLGATQSRIFFTISLPQAWRSVVSGFIMMFARGMSEFGAVVIIAYHPMIAPV
                     LIYDRFNTYGLSAARPASIIFILVALFFFVLFRVLATERRQIFRREDDAAD"
     gene            5141..6217
                     /locus_tag="PLA17_06275"
     CDS             5141..6217
                     /locus_tag="PLA17_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK85057.1"
                     /translation="MMLRTEKLCKRLDTFLIQDISLEVSAGAYYVLLGRSGAGKTQLL
                     ELICGLTTPDKGRIFLEGKDITHKRVQNRGIGLVFQDLALFPHFSVKDNIMYPLKVKK
                     LPLKERVERVQRAAEEMNIADLLLRKPENLSGGEKQRVALARTLVTQPKILLLDEPMA
                     SVDASLKDSIMRMLRRLNRNGQTIVHVTHDFSEAISLASRVGVMHNGQIIQEGTPDEV
                     FTNPVNKFVARYAGIKNFFRVIFVRSYNCCSCITDNRIKFKLPKGDYPSSGLLIIPAS
                     EVLLSRQGAAGVQINNIHGVIEDIVPSPLGYEVTVNAGDHFFADITRREMDEQKYVTG
                     EPVIISFRPESLKLVGEGEEMNKY"
     gene            6225..7400
                     /locus_tag="PLA17_06280"
     CDS             6225..7400
                     /locus_tag="PLA17_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPK85058.1"
                     /translation="MITFEEAFRIVTESSFRSGEEVVSLAEAAGRALTLPVRSDMDMP
                     PWNKSSVDGYACRHDDLGDELTVLETIAAGVMPQKEVVPGTCSRIMTGAAVPEGADRV
                     FMLEESEELPDGRVKFTGRAGKDNIAKAGEDIKKGQTVLRPGHYIKPQDIAVLATVGI
                     MEVTVSCRPRIGVISTGDELVEPDVVPAGAQIRNSNASQLMAQIVRAGAIGKYYGIAR
                     DDEKSITSLMKQALEENDVVLISGGVSFGDFDLVPRAMRSLGLTIHFDQVAMQPGKPL
                     TFCTGENKAVFGLPGNPVSVFVQFEVMVRAFLDRMAGVSEQVPLLQMPLADNYSRKKA
                     ERMSWIPCTITATGEAMPLEYHGSAHISALTGAWGVIIIPRGQNWIQKGEMVSVRQI"
     gene            7417..8184
                     /locus_tag="PLA17_06285"
     CDS             7417..8184
                     /locus_tag="PLA17_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK85059.1"
                     /translation="MRISVTDRCNLRCRYCMPEEGVRLIRHEDILTYDEITAFTRVAV
                     AAGISKVRITGGEPLVRKGVTGLVRMIAAIDGVTDLSMTTNGILLAAYATELAEAGLM
                     RVNVSLDSIDPVKYSYVTRGGNLYSVLEGIEAAQKAGLRPVKINCVIKSRPDESDALK
                     VAEYCMKEGLEVRFIRQMDLSAGTFSVVHGGSGGHCAKCNRLRLTPEGMLLPCLFSDS
                     RFSIRELGPEEALRQALDAKPEMGTANHVNGFYNIGG"
     gene            8189..8644
                     /gene="moaC"
                     /locus_tag="PLA17_06290"
     CDS             8189..8644
                     /gene="moaC"
                     /locus_tag="PLA17_06290"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPK85060.1"
                     /translation="MAKLTHSDESGRATMVFTGEKPEQRRTATASGRIDMQPETLKLV
                     ADNMMKKGDVITVAEIAGIQAAKQCSKLIPLCHNITLDQVSVRIEPDAKGMAASATVR
                     CTGRTGVEMEALTAVSVALLTLYDMCKAVDKTMVISDIKLINKEKHDIT"
     gene            8631..9143
                     /locus_tag="PLA17_06295"
     CDS             8631..9143
                     /locus_tag="PLA17_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPK85061.1"
                     /translation="MTLPEKFRVHVITLSDRAHSGDYTDLSGPAVVKYVKYAMAAAGW
                     QCEIRTSVLPDEAVALHAALLAKPAADLIITTGGTGIGPRDITPEVVKPLLTKEIPGI
                     MEQIRVKYGMKNPGALLSRGVAGMIGNTLIYTLPGSVKAVHEYMAEIVKTMEHTFCML
                     YGIDTHGKRK"
     gene            complement(9210..10517)
                     /locus_tag="PLA17_06300"
     CDS             complement(9210..10517)
                     /locus_tag="PLA17_06300"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HPK85062.1"
                     /translation="MAKSFPRIKVLSLSVLFLSCSLFSLSSPDFSPVEFADENVKSVC
                     FEKEGWKLAYPFLQPGSGDEMTLSFDLLGGSAGTLWYNITHCDRNWNKSDLFTSDYLE
                     GYDENQVTDFLPSFNTRVNYTHYRLTLPNNDVRFLISGNYVITLWEASKPDKPLLIRR
                     FYISEGSSSAAVVFRRPMKPGTTDTHQQAEITLTTGSLPVTDPYRQVTLTIMQNGRAD
                     RMHADLTPDFVGNGRLVYNTLSDKTLFPGGNEFRYFDIKTVRQTRQNVRAIDFVNGLY
                     NAFLIPSDNREFKPYFFNEDFNGKSVVAIEESSDPDREADYLWVWFTLPAYQELQGGS
                     LYVMGDFCSWQYTADNRMFWNAAKGCYEASLLLKQGWYNYEFAFLPDGASVSEGFHFE
                     GSHWETENDYLVFTYFRDPTTRYDRLTGITLANTRSRQAVNRQ"
     gene            10671..>11169
                     /locus_tag="PLA17_06305"
     CDS             10671..>11169
                     /locus_tag="PLA17_06305"
                     /inference="COORDINATES: protein motif:HMM:NF017689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HPK85063.1"
                     /translation="MSTLIRLRKGLNIKLAGKAEKILLPEVPETRFGVRFADFPGLVA
                     RLDVKEGDKVMCGSVLFHDKAFPEIKYVSPVSGVVRKIIRGEKRVLTEVVIGQEGSEK
                     IDFGSAAPLSLAREQVKEKLLNSGMWPVLRQRPYHIVARPDIVPRDIFISVFDTAPLA
                     PDLDYV"
BASE COUNT         2764 a         2714 c         3089 g         2602 t
ORIGIN      
        1 cgacgggctg tttgatgagg acggtccgga tgacctcgac ggcgacggaa acatcagcac
       61 gatgagaata agggataccc tcggcaggct aaggagcgat cctgctgaca agagactgat
      121 gatcacagtc aagcccggtg agaagggtga atggacactg ctcgggcagg aggggataga
      181 caatgacggc gacggcctga tcaacgagga tggcgaaggg tatgtcgacg gaaaccgcaa
      241 ttggggaatg aactggaagc ctaattatgt gcagagggga gcgggaaact accccttcga
      301 gggcaagggc atcagggcca tcgccgaatg gatgctggca aggcccaata ttatcctcgt
      361 ctttgccttc cacaataacg gaggcatgta tctcaggggg ccaacctcaa aggaagctgg
      421 cgagcttccg caggaagacg ttgcagtata tgactacctt ggagagcaca tggagaagat
      481 cgtacccgga taccggtacc tgatcagctg gaaggacctc tatccgactt acggcgactt
      541 tactgatttt acagacaaga ttattgggtc atactcactg gtgggagagc tgttccagcc
      601 tgcaacagag acctatgacg gcacgttgaa gagggttgaa gaagagcgtt caagaagtgg
      661 agatccctcg gcaggtgacc ggcagaggct gttgtttgat gacaacgtta accagggcga
      721 gctctataaa gagtggacgg ctttcaaaca cccggtttac ggtgatatag agattggagg
      781 gtggacgaaa atgagttcgc gtctgccgca tccgttcatg ctgcaggacc tggtgcacag
      841 gaatgcttct tcagtaatct tcgccgcaag gcagactccg agagttacga tggatgtgtt
      901 tgaagtgaag aagattggcg gtgacctgtg gagtgtaagg gtgaggctgg tgaacagcgg
      961 cgccataccg tcagttacct atgaaacgat acgaaataaa ctgtatcctg ttgataagtt
     1021 gactgttaca ggacggaacg ctaaagtggc cagtggcggt gtgctgacca atgcatggat
     1081 gaatcaggtc aattacaagg agtatcgccc cgaggtacag atgtgccagg tgccgggtat
     1141 ggggaaggtg gagtaccagt tcctgattag cggcaggggt gatattgaga tcaggtacga
     1201 gtcgcgcaag gctggctccg tcagcaagac ggtaacgctg aaataattcc gcgaagcgga
     1261 tcaggcattt gcggatcagg ccaatcagtg ttttccccgc acagaggata aagcattata
     1321 ctggtattta cccgcgaaat aatggggtgg ggaaggactg ctgctggtct gcaatgtctg
     1381 cagccctccg ggctatttac cgaatctctc cgtaacaagg gccggccatt cgaggtcaag
     1441 ctccttcagt gtcttcagta ccacctcggc aacggtatag agtttctgcc agtttttgtc
     1501 agccggcact acatgccatg gaacatcatt gcagcgcgtc agaatcttct gatacacctt
     1561 cagatactgg tcgaactttt cgcgtgtatc ccagtcgccg tccttgtgct tccaatgttt
     1621 ttcctccatc tctatacgct ccatcagcct ctcttcctga gtctccttcg acacgttaag
     1681 gaagaattta agaaccttcg tgccatgatc ctcgagcatc ttctcaaagg tatttatcgt
     1741 ttcatacctt ttctcaataa tgtctgcggg gatatatttt tctactgacg gtaccaggat
     1801 atcttcataa tgagatctta tgaacacctt cataatacct ttggcagggc agacgttatg
     1861 aaccctccag aggaagtcgt gcgcatattc caggtcagta ggctttttga aactgtacac
     1921 ctctgttccc acagggttgc agaatttcag gattcccttt gccaccccgt ccttgcctga
     1981 ggcatctatg ccctgaagca acacaatgat gctgtatttg ccctgggcat acatcttgta
     2041 ctgaagctca cctatctcct caagcatctt atccgtcttc ttttttatct ccttcttatc
     2101 ccattccctg ttgagtgtgg ggtgttctgg aattttcatg gcagtactct tttatgtttt
     2161 tcgtccgtaa aagtaggaat aaaggctgaa tagtgcagtt gccgaatctc agaatactat
     2221 ttataatatc ctctttgggg cataataaat caatgattgc cggcccgccg ggctgatgga
     2281 gcacgcgatc tccggagcgt cgggttgccg gaatacctgg cttccagacc gccggattgc
     2341 acaaccgcat gattaccaga ctgcttgacg gcacagccac cgctctgccg gagcacacga
     2401 cttccaaagc accatgctgc cggagcgccg ggctaccgga atacacgact accggtctgc
     2461 cagagcactg cttaatagtc agagcaccga actgtatgac tgccaaacta ttagacagcc
     2521 gaaccacgaa actgcacgac tgtcagaccg caaactgtca ggctaccaaa ctaccgggct
     2581 gtaagacctc atggcggagc gaagcgatct cccttctgac tttccccgga ggagttggcg
     2641 gcattaggca aaaaatgtaa atttgccaca cactaaaaaa atcggatatg ataaaggtaa
     2701 atgagtattt cgaaggaaat gtaaaatcga tggcacttga gaacgaagat ggccgcgcaa
     2761 cggtaggtgt aatggcagca ggtgatttcg agtttggcac ctctaccgtc gagtatatga
     2821 cagtggtgtc aggtaaaatg accgtactcc tcccaggtga aacggagtgg aaaacatacg
     2881 ttccctttca gactttcgtg gtacccgcaa accagaaatt caaactgaag atggatcagc
     2941 ctgccgcata caggtgcctc tataaataaa cagccttacc aatggcagga cataagggag
     3001 atccctacta tgatattttt ctcaggtacg agatgtggct tgagacaatc ggaaaggaac
     3061 gcatcctcga cggtgaggga tttgacctgc tggttgatct tgaccggaca ggatcactca
     3121 ctgcaaccgc ccgtaacctg ggaatgagtt accggaaggc ctggggcatg cttaacgacg
     3181 tggaacggca cctgggcttc aggctgacgg agaagagacg cggcggagca tccggcggca
     3241 ggactctcct gtcagaaaag gggcagcaac tggtgatggc ctacaacagt ctccgccatg
     3301 agactgacat agccatgaag agcatagcaa aagtcttctt taaccgaatt aatgaaataa
     3361 acgggctgaa atgagaacgg tactgttggc actggcagta atgtctgtcc tgttttcggg
     3421 gtgtgcggga ggcagcggca gcggtcgcgg cgataataag cttatcatct ttcatgccgg
     3481 cagcctctcg gtaccggtca gggagctctc cgcagcgttc atgaaagaga atcccggtgt
     3541 taccattctt accgaggcag ccggaagcct tcatgcagcc cgcaagataa ccgagctgaa
     3601 gaagccgtgt gacatcatgc tttcagctga ccattttgtt gtaagcagcc ttcttgttcc
     3661 ggaatatgca tcgtggaata tccgttttgc aacaaacgag atcgttattg catacagggc
     3721 ggagtcaaag tattcctcga tcttccgcga agacaactgg gctgacatcc ttctgaagga
     3781 tgatgtcata atgggcaggt ctgatcctga tgccgatcca tgcgggtacc gcacggtctt
     3841 cgccgcgaag ctggcggaag agtattatgg caggcccggg cttgctgatt cgctgctcag
     3901 aaagaacacc gaatatataa gaccgaagga ggttgatctg gtggcgcttg tctcttccgg
     3961 tgtgattgat tatatgttcc agtataaatc tgtcgccatc cagcatgggt tcgggtacct
     4021 ggaactgcct gatgagatca gcctctcgtc accgctgctg acggagcggt acggaactgt
     4081 ttcatacatg atccccggtg agacaccagg tatccgcaag gaggtatgtg gtgactacat
     4141 cagctattcc gggacggttc ttgaccgggc accgcagaag gagctggcaa tgaagttttt
     4201 cgaattcatg cttggcgaag agggtgccgc catcttcagg aagtgcgggc aggagccgct
     4261 tgttccggct gttgccgatg acactgatgc agtgccttca tcactgagga aattcattgc
     4321 tgttgtgccg gcaaagtaat caaggcgatg aagaaccata cgctgttcaa ctggatcttt
     4381 acattgctgg catcgctggt gcttcttttt atcatagcac cactgatcgg catgttcctg
     4441 catacctctg ctgaagagat gcgcagcact gcctcagatc cggttgtcat acgcagtgtg
     4501 tggtacaccc tggccatcgc ctttgcggca acaatcctct ttgcaattgg tgccgtgccc
     4561 ctggcatgga tcatggcacg caggaagttc cctctgaaga atattgtgca gggtatcatt
     4621 gacctgcctg tggtactgcc tcacacagca gcgggcatag cattgctggg ttttatctca
     4681 cgtgaaggat tcctgggcgg tatagctcag aaagcgggta tactgcttgt caacaacgga
     4741 gccggtatag ccctggccat ggccttcgtc agtcttccct tccttatcaa tgctgcacgc
     4801 gacggtttcg cggcagtgcc ggaacgtacg gagaaggcgg cgctgatgct cggcgcaaca
     4861 cagtcgcgca tattctttac catctcgctg ccccaggcat ggcggtcagt ggtgtcgggc
     4921 tttataatga tgtttgcccg cggaatgagc gagttcgggg cggtggtgat tatcgcctac
     4981 catcccatga ttgcccccgt gctgatctat gaccgcttca acacctacgg cctcagcgcc
     5041 gccagacccg cttcaataat atttatcctt gtggccctgt tcttcttcgt gctcttcagg
     5101 gtactcgcca cagaacgccg ccagatattc aggagggagg atgatgctgc ggactgaaaa
     5161 actgtgcaaa agactcgaca ccttcctgat ccaggatatt tcactggagg tgtcagcagg
     5221 tgcttactac gtgctgctgg gtcgctccgg cgcaggcaaa acacagctcc tcgagctcat
     5281 ctgcggcctc actacccctg acaagggaag aatattcctg gagggtaagg atattacgca
     5341 caagcgggta cagaaccggg gcatcggact ggtattccag gacctggccc tctttccgca
     5401 tttttcggtg aaggacaata tcatgtaccc gctgaaggtg aagaaactcc cccttaagga
     5461 gagggttgag agggtgcagc gcgctgctga agagatgaac attgccgacc tgctgcttcg
     5521 caagccggaa aacctctcgg gcggggaaaa acagagggtg gcgctggcaa ggacactggt
     5581 gacgcaaccg aagatactcc tgcttgatga gccgatggct tctgtagatg ccagcctgaa
     5641 ggacagcatc atgcggatgc ttcgccgcct caacaggaac ggccagacga tagtgcatgt
     5701 cactcatgac ttcagcgagg cgataagtct tgccagcagg gtgggtgtga tgcacaacgg
     5761 gcaaatcatt caggaaggga cccctgacga ggtgttcaca aatccggtca acaagttcgt
     5821 ggcccgctat gccgggataa aaaacttctt ccgtgtcatt tttgtaagat catataactg
     5881 ttgctcatgc ataactgaca acagaataaa attcaaattg ccgaagggtg attacccttc
     5941 ttcagggctg ctgataattc ctgccagcga ggtacttctc tcgaggcagg gtgcagccgg
     6001 agtgcagatt aacaatattc acggtgtcat cgaagacatc gtgccatcac ccctgggcta
     6061 cgaagtcacc gtcaatgccg gcgatcactt ttttgctgac atcacgcgcc gtgagatgga
     6121 tgaacagaaa tatgtaacag gagaaccggt aatcatttct tttcgtcccg aatcgttgaa
     6181 actggtaggg gaaggagaag agatgaataa atactgaaac tattatgata acatttgagg
     6241 aagcgttcag gattgtgacg gaatcatcat tccgttcagg agaggaggtt gtcagccttg
     6301 cagaggctgc cggacgtgct ttgacgttgc cggtgaggag tgacatggac atgcctccgt
     6361 ggaacaagag ctccgttgac ggctatgcct gccgtcatga tgatcttgga gatgagctta
     6421 ctgtgcttga gactattgct gcaggtgtga tgcctcagaa ggaggttgtg ccgggcacat
     6481 gctcgcgcat aatgactggc gctgccgtac ccgaaggcgc tgaccgggtg ttcatgcttg
     6541 aggagtcgga ggaactgcct gacggacggg taaagttcac cggcagagcc gggaaagaca
     6601 atattgcgaa agcgggggaa gatataaaga agggccagac tgtcctgcgc cccggacatt
     6661 atattaaacc gcaggacata gctgtgctgg cgactgtggg cattatggag gtgaccgtca
     6721 gctgtcgtcc ccggattggt gtcatcagca caggtgatga gctggttgaa ccggacgttg
     6781 ttccggccgg agcgcagata cgcaacagca atgcttcgca gctcatggca cagatagtca
     6841 gggcaggcgc cattggcaaa tattacggca ttgcccgcga tgatgagaaa tccatcacat
     6901 cactgatgaa gcaggcgctt gaggagaacg atgtggtgct tatatccggc ggtgtctcgt
     6961 tcggtgattt cgacctcgtt ccccgggcaa tgcgttccct gggactgacc attcactttg
     7021 accaggttgc catgcagccc ggcaaaccgc tcacattctg cacgggggag aataaggctg
     7081 tcttcggtct tccgggtaac ccggtctcgg tcttcgtaca gtttgaagtg atggtcaggg
     7141 cattcctcga caggatggcg ggagtatcag aacaggtacc gctgcttcag atgcccctgg
     7201 cagacaacta ctcacgcaag aaggcggaga ggatgtcatg gataccatgc accatcaccg
     7261 caaccggcga ggcgatgccg ttggagtacc acggctccgc ccacatttct gccctgacgg
     7321 gagcatgggg tgtaattatc ataccccgcg gacagaactg gattcagaaa ggagaaatgg
     7381 taagtgttag acagatataa cagaaatatt aactatttgc gcatatcggt cactgaccgt
     7441 tgcaacctgc gctgccgcta ctgtatgccc gaggagggag tcaggcttat caggcatgag
     7501 gatatactca cctatgatga gatcactgcc ttcacccgtg tggcagtggc tgcaggcata
     7561 agcaaggtac gaatcaccgg cggtgagccg ctggtacgga agggggtgac cggactggtt
     7621 cgcatgattg cggctattga tggggtgaca gacctctcga tgaccaccaa cggcatcctg
     7681 ctggccgcat atgccacgga actggcagag gcagggttga tgagggtcaa tgtcagcctt
     7741 gactccattg accccgtcaa atacagttat gtcacccggg gcggcaacct ttactccgtt
     7801 ctggaaggaa ttgaagctgc acagaaagcc gggctgcgtc ctgtaaaaat caattgtgtc
     7861 ataaagagca ggcccgacga gtcggatgcc ctcaaggttg cagagtattg tatgaaagag
     7921 gggctggagg tccggttcat caggcagatg gacctgtctg ccggcacctt ttcagtggtg
     7981 cacgggggca gcggcggaca ctgcgcgaaa tgcaacaggc ttcgtcttac acccgaaggc
     8041 atgttgcttc catgcctttt cagcgacagt cggttcagca tacgcgaact gggtcctgag
     8101 gaagccctgc gccaggccct ggatgcaaaa cctgaaatgg gcacggcaaa tcatgtaaat
     8161 ggcttttaca acataggagg atgaaattat ggcaaagctg acacacagtg atgaaagtgg
     8221 aagagcaact atggtcttca ccggggaaaa accggagcag agacggacag caacggcgtc
     8281 aggcaggata gatatgcagc cggaaaccct gaagctggtg gcagacaaca tgatgaaaaa
     8341 gggtgatgtc attaccgtgg ctgagatagc aggcatacag gcggccaaac agtgcagtaa
     8401 gcttatcccg ctgtgtcata atatcaccct tgaccaggtc agtgtaagaa ttgagcctga
     8461 tgcgaaagga atggcagcca gtgcaacagt gcgctgcacc ggacgaacag gcgtggagat
     8521 ggaggcgctg acagcggtga gcgtggccct gctgacacta tatgatatgt gcaaggcggt
     8581 tgacaaaacg atggttattt ccgatatcaa actgataaac aaagagaaac atgacattac
     8641 ctgaaaaatt ccgggttcat gtgatcaccc tgagcgacag ggctcacagt ggagactata
     8701 ccgatctgag cggtccggcc gtggttaaat atgttaagta tgccatggcg gctgcaggct
     8761 ggcagtgcga gatcagaaca agtgtccttc ccgatgaggc tgtggccctg catgccgctc
     8821 tcctcgcgaa gccggcagca gaccttatca taaccaccgg tggcaccggt atcggcccga
     8881 gagatatcac ccctgaagtg gtgaagcctc ttcttacgaa ggagataccg ggtatcatgg
     8941 aacagatcag ggtgaagtac ggcatgaaga atcccggggc tttgctgagc cgcggggtgg
     9001 caggtatgat aggcaacacc cttatctata ctctccccgg gtcagttaag gcagtacatg
     9061 agtatatggc cgagattgta aagaccatgg agcatacttt ctgcatgctc tacggtattg
     9121 atacacacgg caaaaggaaa tagccgtcat ccgtcagaca atagtctgaa taactgcaca
     9181 ttacgcaggc tccgcacaga tcgtcaacac tattgcctgt tgactgcctg ccggcttctg
     9241 gtatttgcca gggtaatacc cgtgagcctg tcatacctcg ttgtggggtc acggaaatag
     9301 gtgaacacca ggtaatcatt ttccgtctcc cagtggctgc cttcgaaatg gaatccctca
     9361 gacactgacg ccccgtcagg cagaaaggca aactcataat tataccatcc ctgtttaagc
     9421 agcagcgaag cctcgtagca tcctttggct gcattccaga acatccggtt atcagcagtg
     9481 tactgccatg agcagaagtc tcccatcaca tagagtgatc cgccctgcag ctcctggtat
     9541 gccggcagcg tgaaccatac ccacaggtaa tcagcctccc ggtccgggtc actgctctcc
     9601 tctattgcaa caaccgactt gccattgaag tcctcattga agaaataggg tttgaattcc
     9661 ctgttgtcag aaggaatgag aaacgcattg tacaagccat ttacaaagtc aatggccctg
     9721 acattctgcc tggtctgcct taccgttttg atgtcaaagt acctgaattc attaccgccg
     9781 ggaaataaag ttttgtctga caaggtgttg tataccagtc tgccgttgcc tacgaagtca
     9841 ggggtaaggt cggcatgcat tctgtcagcc ctgccgttct gcattatggt cagggtcacc
     9901 tgccggtaag ggtctgttac cggaaggctg ccagtggtga gagtgatttc ggcctgctgg
     9961 tgcgtatcgg tggtgccggg tttcatcggc ctgcgaaaga ccacggcggc tgaggagctc
    10021 ccctcgctga tatagaaacg cctgataagc agtggcttgt ctggcttgct ggcctcccag
    10081 agtgtgatga catagttgcc tgatatcagg aagcggacat cgttattcgg aagggtgaga
    10141 cggtagtggg tgtagttcac cctcgtgttg aacgaaggca agaagtcagt cacctgattc
    10201 tcatcgtatc cctccaggta gtcgcttgta aaaaggtcgc tcttgttcca gtttcggtca
    10261 caatgtgtta tgttgtacca gagagttccg gcactgccgc caaggagatc gaagctgaga
    10321 gtcatctcat cacctgatcc cggctgcagg aaagggtatg cgagcttcca gccctctttt
    10381 tcaaaacaga cactctttac attctcatca gcaaattcta ccggtgaaaa atccggagat
    10441 gacaaagaaa agagcgaaca ggagagaaac aatacagata aacttaatac ttttatgcgc
    10501 gggaaagatt ttgccattat ctctgtttta ggactaaaat acaaatatcc tgccgctatc
    10561 tgaacaatgg cagtcaaaaa aagttatgtg ataaagatca attttcctgt gataatcctg
    10621 acaatgttag tataattgcc atcttttaag gaattaagat taattctgat atgtcaactc
    10681 tgatcagatt acgtaaggga ttaaacatta agcttgccgg gaaggcggag aaaattcttc
    10741 tccccgaggt gcctgagacc cgtttcgggg tcaggtttgc tgattttccg ggtcttgttg
    10801 cccgtctcga tgtgaaggag ggagacaaag tcatgtgcgg cagtgtgctg tttcatgaca
    10861 aggcatttcc tgagataaag tatgtatcac cggtaagcgg cgtggtccgg aaaattatcc
    10921 gtggtgagaa gcgagtgctt accgaggtgg tgatcgggca ggagggatct gaaaagatcg
    10981 atttcggcag tgccgcccct ctcagccttg cacgggaaca ggttaaggag aagcttctga
    11041 actccggtat gtggcctgtt ctgaggcagc gtccgtatca tattgtggcc aggcctgaca
    11101 ttgttccgag agatatcttc atctctgtat ttgacaccgc tccgcttgct cctgatcttg
    11161 actatgtga
//
LOCUS       DAOPBK010000084        11167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5712_length_11167_cov_4.546706, whole genome shotgun sequence.
ACCESSION   DAOPBK010000084 DAOPBK010000000
VERSION     DAOPBK010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5712_length_11167_cov_4.546706"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLA17_06310"
     CDS             <1..406
                     /locus_tag="PLA17_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPK85064.1"
                     /translation="ISPEEILKMKMEEFYKIQHNVTPIEPVIALVKKYHGRLPMSVGT
                     GGHREAVERTLEIVGIRHYFDIIVTAHDVTRHKPHPDTFLRCAELMGVAPEECEVFED
                     GDLGLRAARNAGMIATDVSKWYESTWTKDLKV"
     gene            478..1137
                     /gene="mnmD"
                     /locus_tag="PLA17_06315"
     CDS             478..1137
                     /gene="mnmD"
                     /locus_tag="PLA17_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HPK85065.1"
                     /translation="METKLIITDDGSHTLYVPGMNEHYHSRFGAMTESRHIFINAGLA
                     TLPPGDTAILEVGFGTGLNALLTAIYAGDEKIRVSYTTLEKEPLEASIITSLNYGTLA
                     GKGGEELFMAIHEAPWNRSCAITEWFTIEKRLTDLITDELSGLYDLIYFDAFGPDKQP
                     AMWNENVMRKIAAVTHPGSLFVTYSAKGDLKRMLRMLGFDVTILPGPPGKRVITRALK
                     K"
     gene            1213..1902
                     /locus_tag="PLA17_06320"
     CDS             1213..1902
                     /locus_tag="PLA17_06320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPK85066.1"
                     /translation="MKVIRLILVMALAAIVVLPATGQKSKFASTEDSLSYALGVANYM
                     YYAGDSININTKMFSKGMSDASKERALMNDTAASAFIVSYMQRREKARLMAEYSQRIE
                     AAKAWLADNAMKEGVITSPSGLQYKVLQEGTGATPGPEDIVKVHYTGKTIEGNKFDSS
                     YDRGEPAQFRVSNVIRGWVEGLQLMKEGSRMMLYIPYDLAYGERGAGNIIKPFETLIF
                     EVELFEVIKEE"
     gene            1964..2662
                     /locus_tag="PLA17_06325"
     CDS             1964..2662
                     /locus_tag="PLA17_06325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPK85067.1"
                     /translation="MKIKGLVILAVATAMIVSSCNTESSITSTKLKSPEDSLAYALGI
                     ANYFSYMAADSIDIDPVLQAKGMLDAKKGKNTLDEQMAQGYVMMYMQNRQREKIKAMY
                     SKNIDEGEKFLAENKKREGVQETTSGLQYEVVTMGTGEKPGPSDVVKVHYTGKLLDDT
                     TFDSSIDRGEPVTFGVNQVIMGWQEGLQLMPAGSKFKLYIPYELAYGDQGQGPIPPFS
                     TLIFDVELIEIVEQ"
     gene            2659..3120
                     /locus_tag="PLA17_06330"
     CDS             2659..3120
                     /locus_tag="PLA17_06330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK85068.1"
                     /translation="MISAEIHTPKGVMKALLYDDDAPGTVSNFVKLAREGFYDGLAFH
                     RVIPDFVIQGGCPFSRNPGDPKIGTGGPGYRIKCELTGRKQYHDRGVMSMAHAGRDTG
                     GSQFFICHSRANTKHLDRQHTCFGKVVEGVEVIDLIRAGDMIEKIVIEESE"
     gene            3120..3503
                     /locus_tag="PLA17_06335"
     CDS             3120..3503
                     /locus_tag="PLA17_06335"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HPK85069.1"
                     /translation="MEYQITGRVAEVYPVNRISERFRKREFVIEHKDSSSKQVYVDYI
                     KFQLTQDRCEIIDESWLRQDVTVTFNIRGNRWEKNGVANYITNLNALSVTRGIAVAEN
                     GQKAIVSPQLEDAPSPSPEFDDLPF"
     gene            3706..5748
                     /locus_tag="PLA17_06340"
     CDS             3706..5748
                     /locus_tag="PLA17_06340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPK85070.1"
                     /translation="MTPDNPLLQDWPTPFGIPPFNLIRPEHFKPAVEEAISIAGKEID
                     DIVLNPAEPDFRNTIEAIEEAGSLLSRITPVLFNLNSSDTTPELQNAAREVSPVLTAF
                     SNDILLNPALFARVKSVYEKRDSLDLNQEEMILLYRKYRNFIRGGAGLAENDKERFRA
                     IAVELSTLSLRFEENILAETNDFTLHLTSEEDLSGLPEGVREAASALAGEKGMEGWLF
                     TLHAPSFVPFMQYADRRDLREKLFRAYSRRAFRGNEHDNRELVFRITELRLEMARLLG
                     YSNYAAYALEERMASTPEKVNRFLRTLLEASLPAGNRDLGDIEEYAARHGHEGRLERW
                     DWAYWSEKLRMERFSIDDEALRPYMPLDKVREAVLGLATRLYGLSFLENRSIPVYNEE
                     VSAFEVHDGERGLTAILMLDFHPRKGKSGGAWMTGFREQRKEGSERVIPVVSLVMNFT
                     RPTPARPSLLSHSEMNTFLHEFGHALHGMLSDCTYESLAGTNVKRDFVELPSQIMENW
                     AWEKEWLDTWAAHYQTGEKIPGEMVQKLRDSLTYNEGYACMRQLSFGLLDMAWHTLEE
                     PHTGDIVTFERSAMRPAELLPAAEGACMSVSFAHLFSGGYAAGYYGYKWAEVLDADAF
                     SLFSERGIFDRETAGSFRHNILERGGSREPDELFRSFRGREPSIEPLIERSGFRKK"
     gene            complement(5916..7844)
                     /locus_tag="PLA17_06345"
     CDS             complement(5916..7844)
                     /locus_tag="PLA17_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPK85071.1"
                     /translation="MKRILSITLGLIIMLGFSSCDKWLDVNVDPDNPNDLSATPEIRL
                     PWIQHYYMYAWGTANMRTSTIAGIMTQTTSATGGNSRLAAWNPIQGSCTTIYQNWFLG
                     GAVNLKPLIDRAEEQGAWHYMGAAYCIKAMGFMMMLDLYGEMPYTEALTGKYNPAYDQ
                     GDVIYNGCMADLDRAIEYFGKTQEPGAVALSKGDSWNGGDVNKWLKLCYGLKARYLLQ
                     ISKKSTFDPAAVLAALQNAPQSNADNIIMKHYNVEGDAVNFTVADPYQTNSTWNSAAY
                     GTGQRYTRWYANLLTNNFTGGSGVTDPRLPKLLPAVMTNTELNASGNIINYEWRRDIG
                     VDAMNSSVRVNSGPLNYSYANSPQTLKYTIADEAARDAFIAAIPHPYTVDGNDVSVTY
                     PRGSFYVNSTNYLRAGDTAYVNMRSNSMSTSGLSATDMYYYLNADVKAVGGTGSFFVR
                     PDSDSDILTYAEMCFIRAEVYLRQGNAPQAHNAYIAGIEASFDRMQTKLNEWKSAGTV
                     NPDQMPMDEADIAAYMASAAVVQNASNLTMADIIQQKIIAMGINMQIWNDMRRFNYSA
                     GNIGNFGIVYRDYKRPYEFTATNIIPGTSPTDLNYWFRRFNHSAHESNYNNEQLLLSN
                     PLAMQDAIWSDPVWWDKE"
     gene            complement(7868..10999)
                     /locus_tag="PLA17_06350"
     CDS             complement(7868..10999)
                     /locus_tag="PLA17_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPK85072.1"
                     /translation="MFSAILLILFSGTIISQTLRVSGKVIDNAGLALPGVKIVLEGTI
                     TGVASDSDGLYSINAPANGTLVFSFIGMETVKIPIDGRTTINVTMTESSVLVEEVVVT
                     ALGIKKEKKALGYSVQDLKSDEILKNKQTNVINSLAAKVAGVSITQSSGSAGAGSTII
                     IRGGNSASETRDNQPLFVVDGIIYDNSTINSGNSGTDGVTKNATSFSNRVMDINPDDI
                     ESMSILKGAAAAALYGSRAADGVVVITTKKGGAGPVRVDFNSKYSYSWVGTRPELQDV
                     YGRGYYNEAGSFSDYTTQSWGNPITGPVYDNVGNFFQGGNVFDNSLSISGGSPNSSFY
                     MSASNFDQTGVVPTTGFDKNTFRFNGEQKYGKLTVGANAAYSITNTLKTLTTAGLYNG
                     GGNGTMTALYGWSRSDDIAKYLNPDGTKYRMFEGIQELASDVENPYWILNKNKLTDKV
                     NRFTGGINVSFNVTDWFNILYRVGYDTYNSGAYTYIAPGGAVTEIYQKGRLSKSGSEY
                     AFLTSNLMLNFNQKVGGIDLNLLLGHTAENTDRVSRTNWGYGFSTPGTISFGNIVDDQ
                     KFFTERNVTKRLVGVFGELRASYKSIAYLTVTGRNDWSSTLPVENRSYFYPSVSGSLV
                     FTELLPENDILSFGKIRASWAQVGKDADAYATNTYLWAPQIVSGQFVGTGNSWTGGSP
                     NLVPEIQTSTELGAELRFLEGRLGLDFTWYNSVTKNQLASPRLAQSTGYIFLTLNSGS
                     VQNKGIELSINATPVKKRDFTWDVTLNLSGNRGTLGDFLPGVTFFYVTDVQIGGVKAA
                     SVPNGGYFLGLTGDRWLREKDTDGNEIPDGRYIVDESTGLYAPTTVTTNVVGNREPDF
                     IGGLNNNLQYKNLSFSFLIDIRKGGDIYNGTDWYLTTRGLSMKTLDRKSVTVSGVSKT
                     SGEPVTYTYEAGKTYTVGGATKSGEYMIQQYWSKYGDNAYNFITSTNWLRLRSVNLSY
                     DFTDLIGKRGFIKGLSASIGGYNLLLWTNYKGMDPEVSVSGSGTGGSGSMGIDYCGVP
                     ATRNVTFGLNATF"
BASE COUNT         2902 a         2751 c         2756 g         2758 t
ORIGIN      
        1 gatttctcct gaagagattc tgaagatgaa gatggaggag ttttataaga ttcagcataa
       61 cgtcaccccg attgagccgg ttattgcact ggtaaaaaaa taccacggca gactgccgat
      121 gtctgtaggc accggcgggc accgcgaggc agtggagaga acgcttgaga tagttgggat
      181 caggcactac tttgatataa tagtgacagc gcacgatgta accaggcata aaccccatcc
      241 cgatactttt ctcaggtgtg cggagctgat gggcgtcgct ccggaagagt gcgaggtctt
      301 tgaagacgga gaccttggac tccgggcagc ccggaacgcc ggtatgatcg ccaccgatgt
      361 aagcaaatgg tatgaatcaa catggactaa agatttgaaa gtttgatggc ttgaactcat
      421 tcggcattca gcgatgattt accgcctgac ggtattgctg attgccggtt ttaatatatg
      481 gaaacaaaac tgatcattac ggatgatggt tcccacacac tctacgtgcc ggggatgaat
      541 gaacattacc actcgcggtt cggggcaatg acagagtcaa gacacatttt catcaatgcc
      601 ggccttgcca ctctcccgcc gggtgatacg gccattcttg aggttggctt cggcaccggt
      661 ctcaacgcgc tcctgaccgc catctatgca ggagatgaaa agatccgtgt cagttacacc
      721 acccttgaga aagaacccct cgaagcatca atcatcacca gtctaaacta cggcaccctg
      781 gcgggcaaag ggggagaaga acttttcatg gcaattcatg aagccccatg gaacagatcg
      841 tgcgcaataa ctgaatggtt cacaatagaa aagaggttga cagaccttat caccgatgaa
      901 ctctcaggtc tttatgacct gatttacttc gatgccttcg ggcctgacaa gcaacccgcg
      961 atgtggaatg agaatgtaat gcgcaaaata gcagccgtga cacaccccgg ctctctattc
     1021 gtaacttact cggccaaagg tgatcttaaa cgaatgctcc ggatgctcgg ttttgatgtc
     1081 acaattctcc ccggacctcc gggaaaaaga gtcataaccc gggcgttgaa aaaatgattt
     1141 atggtatatt tgcgccactg aaacagtgac cggataatga atctgagcct gtaatcacat
     1201 aaaaatgaaa aaatgaaagt aataagactc attcttgtca tggccctggc agcaatcgtt
     1261 gttctgccgg caacaggcca gaaatcaaaa tttgccagca ccgaagactc cctttcatat
     1321 gctcttgggg tagccaacta catgtattac gccggtgaca gcatcaacat caataccaaa
     1381 atgttctcaa aagggatgag tgatgcttca aaggagaggg cgctgatgaa tgacactgcc
     1441 gccagcgctt tcattgtcag ctatatgcaa aggcgtgaga aagcacgcct catggctgag
     1501 tacagccagc ggatagaggc tgcaaaagca tggctcgcag acaatgccat gaaggaagga
     1561 gtaataacct caccaagcgg cctccagtac aaggttctgc aggaagggac aggagcgact
     1621 cccggcccgg aggatatcgt caaggtacat tacacaggaa agaccattga aggaaacaag
     1681 ttcgactcgt cgtatgaccg cggtgagcct gcacagttca gggtcagcaa tgtcatacgc
     1741 ggctgggtgg aaggactcca gctgatgaag gaaggctccc gtatgatgct ttacatcccc
     1801 tatgacctcg cctatggtga aagaggtgcc ggcaacataa tcaaaccgtt tgaaacacta
     1861 atatttgaag tggaactgtt cgaagtgata aaagaagagt aatttccctg acttgacact
     1921 caaaacatta tctttgccaa aaataaattc taaaatatac acaatgaaaa tcaaaggttt
     1981 agttattcta gctgtagcaa cagccatgat agtatcatca tgcaatacag aatcaagcat
     2041 taccagcacc aaattgaaga gccccgaaga ctctctcgca tacgctctcg gtattgccaa
     2101 ctatttttcc tatatggcgg ctgacagcat agacattgat ccggttttgc aggcaaaagg
     2161 aatgcttgac gcaaagaaag gcaagaacac acttgacgag cagatggcac agggatatgt
     2221 gatgatgtac atgcagaaca ggcagaggga gaagataaaa gcaatgtaca gcaagaatat
     2281 cgatgaaggt gaaaagttcc ttgctgagaa caagaaacgc gaaggcgtac aggaaaccac
     2341 cagcgggctt cagtatgagg ttgttacaat gggcaccggt gagaaacccg gcccttcaga
     2401 cgttgtgaag gttcattaca ccggcaaact ccttgatgac accacatttg actcttcaat
     2461 tgaccgtggt gaacctgtca ccttcggcgt caatcaggtt atcatgggat ggcaggaagg
     2521 ccttcagctg atgccggcag gatcaaaatt caaattatac attccttatg agctcgctta
     2581 tggcgatcag ggacagggtc cgattccgcc cttctcaacc ctcatatttg atgttgagct
     2641 catcgagatc gtagagcaat gatatcagcc gagatacata ccccaaaggg ggtgatgaag
     2701 gcactcctct atgatgacga tgcccccggc acagtcagca actttgtcaa actggccagg
     2761 gagggattct atgacggact tgcatttcac agggtcattc ctgatttcgt aatccagggc
     2821 ggttgcccct tctcgaggaa tccgggtgac ccgaagatag gaacaggcgg accgggctac
     2881 aggatcaaat gtgaacttac cggcaggaaa cagtaccatg accgcggtgt gatgtcaatg
     2941 gcgcacgccg gaagagacac cggagggtca cagttcttta tctgtcacag cagggcaaac
     3001 accaaacacc tcgacagaca gcatacatgt ttcgggaagg tagtggaagg agttgaagta
     3061 attgacctga tcagggcagg tgacatgatt gaaaaaattg tcattgaaga atctgaataa
     3121 tggaatacca gatcacaggc agggtcgctg aagtttatcc ggtcaacagg atcagtgaac
     3181 gcttccgcaa gcgggagttc gttatcgagc ataaggacag cagcagcaaa caggtttatg
     3241 ttgactacat caaattccag ctgacacagg acaggtgcga gataattgac gagtcgtggc
     3301 tgagacagga tgtcacagtg actttcaata tccgtggaaa ccgctgggag aagaacgggg
     3361 tggccaatta catcaccaac ctcaacgcac tttcagttac ccgtggcatt gcagtggcag
     3421 aaaatggcca gaaagcaatt gttagcccgc aactggaaga tgccccctcc ccaagccccg
     3481 aatttgacga cctgccgttc tgatttattt cattagcgac ataagagtgc catatccccg
     3541 ggggtgtggc acttttgcta ttcaggcaat agcagcagtg gaaaggggaa aggggaaagt
     3601 taaaagggga aaggggaaag gcgtttgcag gattgcaaac acacattttc cccgaagggg
     3661 attcagatta gtaatgctat atttgtcaaa ccacatctct gacctatgac tcctgacaac
     3721 ccgctcctcc aggactggcc gacccccttc ggcatccctc cctttaacct catcagaccg
     3781 gaacatttca aacctgcagt tgaagaagca atctcaattg ccggaaagga gattgacgat
     3841 atagtattaa acccggcaga gccagatttc cgtaatacca tcgaagccat tgaagaggcc
     3901 ggatcactgc tcagccgcat cacccccgta ctcttcaacc tgaacagctc cgacacaacg
     3961 ccggaactgc agaatgcagc cagggaggta tctcctgttc ttacagcctt cagcaatgac
     4021 atcttactca atcctgcact tttcgccaga gtgaagagcg tgtatgaaaa aagggattca
     4081 cttgacctta accaggaaga gatgatcctt ctctaccgca agtacaggaa cttcatcagg
     4141 ggcggtgccg gactggcaga aaatgacaag gagcgcttca gggccatcgc cgttgaactc
     4201 tccaccctgt cactcaggtt tgaagaaaat attctggctg agaccaatga cttcaccctt
     4261 cacctgacaa gtgaagagga tctctccggt ctcccggaag gtgtccgtga ggcagcctca
     4321 gcccttgcag gtgagaaagg gatggaaggc tggctcttta ccctccatgc tcccagcttt
     4381 gtgcccttca tgcagtatgc agaccgtcgc gacctccgcg agaagctttt cagggcctac
     4441 tcgcgccgag ccttccgcgg aaatgaacat gacaatcgtg agctggtctt caggataaca
     4501 gagctcagac tggagatggc aaggcttctg ggatacagca actatgccgc atacgctctg
     4561 gaagagcgga tggcctccac acccgaaaaa gtgaacaggt tcctccggac ccttcttgaa
     4621 gcttcgttgc cagccggcaa cagagacctc ggggatatcg aggaatatgc tgcacggcac
     4681 ggtcatgaag ggaggcttga acgatgggac tgggcatact ggtcagagaa gcttcgcatg
     4741 gagcggttca gtatagacga tgaagccctg agaccataca tgcctcttga caaggtcagg
     4801 gaggcagtgc tggggctggc gaccaggctt tacggacttt cattcctgga gaacaggagc
     4861 attcccgttt acaatgagga ggtatctgcc ttcgaggtgc atgacggcga gaggggatta
     4921 acagccatac ttatgctcga cttccacccc cggaagggca agagcggggg tgcctggatg
     4981 accggattcc gcgaacagcg gaaagaggga agtgagagag tcatcccggt ggtttcactg
     5041 gtgatgaatt ttacccgccc gacaccggca cgtccgtcac tgctctcaca cagcgagatg
     5101 aacacctttc tgcatgagtt cggccatgcc ctgcatggca tgctctcaga ctgcacctac
     5161 gaaagcctcg ccggaaccaa tgtgaagcgc gactttgtgg agctcccgtc acagataatg
     5221 gagaactggg catgggagaa ggagtggctt gacacgtggg ccgcacatta ccagaccgga
     5281 gaaaagatac ccggggagat ggttcaaaaa ctcagggact cactgaccta caacgaagga
     5341 tacgcatgca tgaggcagct cagtttcgga cttcttgaca tggcatggca tactctggaa
     5401 gagccgcata caggtgacat tgtgaccttt gaacggtcag caatgaggcc tgccgagctt
     5461 ctgccagcag cggaaggcgc ctgcatgagc gtctctttcg cccatctctt ctccggaggc
     5521 tatgctgcag gctattatgg ctacaagtgg gctgaggtgc tcgacgctga cgcattcagc
     5581 ctcttcagtg agagaggcat cttcgacagg gaaacagccg gctctttccg ccacaatatt
     5641 ctcgaaagag gcggcagccg tgagcctgat gagctcttca gatctttccg cggaagggaa
     5701 ccctccatag aaccgctgat agaacgaagc ggattcagaa agaaatgata ccaccggtca
     5761 cctggcagcc agcaactggc agtcagcagc cggcagtcag cagccggcag tcagcaacaa
     5821 attaatgaaa tgctgattat cagcaacaga ctcataacta taaaatgaaa aaccgggggg
     5881 ctatattcag ccccccggat atcagcataa tgaagctatt ccttatccca ccagacagga
     5941 tcactccata ttgcatcctg catggcaagt ggatttgaaa gcaatagctg ttcgttattg
     6001 tagttgcttt cgtgagcgct gtgattgaac ctgcggaacc aatagttcag gtcagtcgga
     6061 ctcgttcctg gaatgatatt ggttgcagtg aactcgtatg gtctcttgta gtccctgtac
     6121 acaataccaa aattgcctat gtttcctgca ctgtaattga aacggcgcat atcgttccaa
     6181 atctgcatat tgatacccat ggcaatgatc ttctgctgaa tgatgtcagc cattgtaagg
     6241 ttactggcat tctgcaccac tgcagcgctg gccatatatg ctgctatgtc tgcctcatcc
     6301 attggcatct gatcaggatt aaccgtcccg gcagacttcc actcattgag tttggtctgc
     6361 atacggtcaa agcttgcctc tattcctgca atataagcat tatgagcctg tggtgcattt
     6421 ccctgtctca ggtacacctc agccctgatg aagcacatct cggcatatgt cagtatatca
     6481 gaatctgaat caggccttac gaagaaacta cctgttccgc cgacagcctt cacgtcggcg
     6541 ttgagatagt aatacatatc cgttgctgac agaccgcttg ttgacatgct gttagacctc
     6601 atgttgacat acgcggtgtc accggcacgt aagtagttgg tgctgttgac atagaatgat
     6661 cccctggggt atgtcactga aacatcattg ccgtcaactg tatatggatg agggattgca
     6721 gcgatgaagg cgtctcttgc tgcctcatca gctattgtgt acttcagggt ctgaggcgaa
     6781 ttggcgtatg aatagttcaa gggtcctgaa ttcactctga cacttgaatt cattgcgtca
     6841 acaccaatat ctctcctcca ttcatagttg ataatgttcc ctgaagcatt caattcagtg
     6901 ttggtcatca cagcaggcag aagctttggc aggcgtgggt ctgtcactcc ggagcctcct
     6961 gtgaaattgt ttgtcagcag gtttgcatac caccttgtgt aacgctgtcc tgttccatat
     7021 gctgcagagt tccatgtgga gttggtctgg tagggatcag caacagtgaa attaaccgca
     7081 tcaccctcca cgttgtagtg tttcatgata atattatcag cattggactg tggtgcattc
     7141 tgcagagctg ccagcactgc tgccgggtca aatgttgatt ttttggaaat ctgcaggaga
     7201 taacgggctt tcagaccgta gcaaagcttg agccacttat tgacatcgcc tccgttccag
     7261 ctatctcctt ttgagagggc tacggcacca ggctcctggg tttttccgaa atattcgatt
     7321 gccctgtcca ggtcagccat acatccgtta tagataacat cgccctgatc ataggctgga
     7381 ttgtatttcc ctgtcaaagc ttcagtataa ggcatttcac cataaaggtc aagcatcatc
     7441 ataaagccca tggccttgat gcaataggca gctcccatgt aatgccaggc cccctgctct
     7501 tcagctctgt caataagagg tttaaggttg acagcgcctc cgaggaacca gttctggtat
     7561 atcgttgtgc aactgccctg tatgggattc caggctgcca gcctgctgtt gcctccggtg
     7621 gcagaggttg tctgagtcat tatccctgca atggtgctcg tacgcatatt tgcagttccc
     7681 caggcataca tgtagtaatg ctgtatccag ggaagcctga tctccggggt agcactgagg
     7741 tcatttggat tgtcagggtc cacattgaca tcgagccact tatcacatga agagaagccc
     7801 aacataatga tcagaccaag tgtaatagat aatatccttt tcataatcaa cttttttgaa
     7861 tcagttatta aaatgttgca ttgagaccga atgtcacgtt tcttgttgca ggtacaccgc
     7921 agtagtcgat acccatcgag ccagacccgc ctgttcctga accggaaacg ctgacttcag
     7981 gatccatccc cttgtagttc gtccagagaa gaaggttata gccgccgatt gaagctgaca
     8041 gacctttgat gaaacccctt ttgcctatga ggtctgtgaa gtcatacgac aggttaacag
     8101 accgcagtct cagccagtta gtgctggtta tgaaattgta agcattatca ccatattttg
     8161 accagtattg ctggatcata tactcgcctg acttcgtggc tccgcctaca gtataggtct
     8221 tgccggcctc ataggtgtat gttacaggct cacccgatgt tttggaaaca cctgatacag
     8281 taactgactt acggtcaagg gttttcatgc ttaatcctct tgttgtcaaa taccagtctg
     8341 tgccgttgta aatatcaccg ccttttctga tgtcaatcag gaaagagaag ctcagattct
     8401 tgtactgcag attgttgttc agacctccga tgaaatcagg ttcacggttt cccaccacgt
     8461 ttgtcgtaac ggttgttggt gcatacagcc cggtactctc atctacaatg tacctgccat
     8521 caggtatctc attcccatcc gtatccttct cccgcagcca acgatcgccg gtcaggccaa
     8581 gaaagtaacc gccgttcggt accgatgctg ccttaacccc tccgatctgt acgtcagtaa
     8641 catagaagaa agtgactccc ggcaggaaat ctcccagagt accccggttg cctgaaaggt
     8701 tcagtgtcac atcccatgtg aagtcacgtt tttttacagg agttgcattg attgacaact
     8761 ctatcccctt gttttgtacc gagccgctgt tcagcgtaag gaagatataa ccggtcgact
     8821 gtgcaagtct ggggcttgcc agctggttct tcgtcacgct gttgtaccag gtgaagtcaa
     8881 gtcccagcct gccctcgagg aaccggagtt cagcccccaa ctcggttgat gtctgaattt
     8941 caggcaccag gttggggctt cctccggtcc agctgttgcc ggtacccaca aactgaccgc
     9001 tcacgatctg aggagcccat agataggtgt tggttgcata tgcgtcagca tccttgccca
     9061 cctgggccca tgaagcacgg atctttccga atgaaaggat atcattttca ggaagcagtt
     9121 cagtaaatac taagctgccg gatactgaag gatagaaata tgatctgttt tcaacaggaa
     9181 gcgttgacga ccagtcgtta cggccggtaa cagtcaggta ggcaatgctc ttgtatgatg
     9241 cacgaagttc accaaataca cctaccaatc gtttggtgac atttctttcg gtaaagaact
     9301 tctggtcatc aactatgttc ccaaagctta ttgtaccggg ggttgagaat ccgtaacccc
     9361 agttggtccg tgaaactctg tctgtatttt cagctgtatg ccccagtagc aggttcaggt
     9421 caataccacc aaccttctga ttaaaattca gcatgaggtt ggaggtaaga aacgcatact
     9481 cggaaccact cttactcagt cgacccttct ggtatatctc tgtcactgca ccaccgggtg
     9541 cgatataggt ataggcaccg ctgttgtatg tatcataacc tacccggtaa agtatgttga
     9601 accaatctgt cacgttgaaa gaaacattga taccgcccgt gaaccggttg accttgtcgg
     9661 taagtttgtt tttattcagt atccagtaag ggttctccac gtcactggca agttcctgaa
     9721 tgccctcaaa catcctgtac tttgttccgt caggattgag gtattttgcg atatcgtcac
     9781 tccgtgacca gccgtagaga gctgtcattg ttccgttgcc tccgccgtta tacagacctg
     9841 ctgtcgtcag agtcttcagg gtgtttgtaa ttgaataggc tgcatttgca ccgacagtca
     9901 gtttgccata tttctgctca ccgttgaagc ggaatgtatt cttgtcaaat cctgtcgtgg
     9961 gcacaactcc cgtctggtca aaatttgatg ccgacatgta gaagctgcta ttggggctgc
    10021 ctcctgagat gctcagactg ttgtcaaata cattcccgcc ctgaaagaag ttgcctacgt
    10081 tgtcatagac tggtccggtt atcgggttac cccatgactg ggttgtatag tcactgaatg
    10141 acccggcctc gttatagtaa ccccttccat aaacatcctg caactcaggc ctggtgccaa
    10201 cccaggaata ggagtatttt gaattgaagt ctacccttac cggacctgca ccccctttct
    10261 tggttgtaat gacaaccacg ccgtcagctg cccgtgagcc atacaatgcg gcggcggctg
    10321 ctcccttaag gattgacatg ctctcgatat catcagggtt gatgtccatc acgcggttgc
    10381 tgaatgatgt tgcatttttt gtcacaccat cagtacctga gtttccgctg ttgatggtag
    10441 agttgtcata gatgatgccg tcaacgacga acaaaggctg gttgtcgcgt gtttcgctgg
    10501 ctgaattgcc gccacggatg atgattgttg atccggcgcc cgcagaaccg cttgactggg
    10561 tgatactcac accagcgacc ttagcagcca gtgagttgat aacatttgtc tgcttattct
    10621 tcaggatctc atcgctttta aggtcctgaa ctgaatatcc cagggccttt ttctccttct
    10681 tgataccaag ggcggtaacc acaacctcct caacaagaac tgatgattcg gtcatggtga
    10741 cattgattgt ggtacggcca tcaatcggaa ttttcaccgt ttccatccct atgaaagaaa
    10801 agaccagtgt gccattagca ggtgcattga tggagtataa gccatcactg tctgaagcca
    10861 caccggtaat ggttccttca aggactattt ttactcccgg aagagccagc ccggcattgt
    10921 caatgacttt cccggagacc cgcagagtct gggaaataat tgttccggaa aacaggatca
    10981 gcaaaatggc tgaaaacatt gcgtgttttc cccatttcgc aacttcccgt tgagagtgca
    11041 attttaggtt tgttctcatg ttattgatat ttggagttaa atggtttggt cttatggggt
    11101 agtgttccaa tcagtgtgtc tggggtggca ggttttcatc gaagttttac atcttaaaat
    11161 tacactt
//
LOCUS       DAOPBK010000085        11061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5774_length_11061_cov_5.107850, whole genome shotgun sequence.
ACCESSION   DAOPBK010000085 DAOPBK010000000
VERSION     DAOPBK010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5774_length_11061_cov_5.107850"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..383)
                     /locus_tag="PLA17_06355"
     CDS             complement(93..383)
                     /locus_tag="PLA17_06355"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPK85073.1"
                     /translation="MRRNKTITLGEALGELIKEYRLEKGLKDAAVLNIWEDIAGKVIT
                     ARTKRIYLKDGVLHIYLTSSVLRNELMMLRDSLKSRINSRAGEEVVREIVLH"
     gene            complement(387..1502)
                     /locus_tag="PLA17_06360"
     CDS             complement(387..1502)
                     /locus_tag="PLA17_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPK85074.1"
                     /translation="MMHLARLELTNFKNYEEVSLEFSPGFNCFVGNNGVGKTNILDAI
                     HYLSLTKSFFYNSDTLSIRHGDDYFMIRGTFVTDDNSDDLFCAFQKTKQKVFKLNGRE
                     YQRMSDHVGKYPVVMLSPSDTTLITGGSEERRRFLNLIISQYDPAYLEAHMRYNKALM
                     QRNRILRDDSANQSGLLEIYDEQMVPEAGMIYNARRSLTENLLPLFRSYYERISGGAE
                     KVSIRYRSHLSSGDFRQQLADSRERDYAMQHTTMGIHRDDLVFEIDGHSARTNASQGQ
                     QKSFIVALKLAKFGLMTRMIGFAPALLLDDIFDKFDQNRVEEIIRLAGSGEFGQVFIT
                     DTQQDRIHRILDNTGVDFRLYRIGKKGIEEEITNGEK"
     gene            complement(1609..3567)
                     /locus_tag="PLA17_06365"
     CDS             complement(1609..3567)
                     /locus_tag="PLA17_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPK85075.1"
                     /translation="MMRIFSVLILLLLATSAVLQAQDSWNRTTWEEKPVYFSLESENQ
                     NENAVGIFYNEKTEYLYNPEGQLEMIHTLHRRIRLNTDDAINGFNKLSVSMADVIEME
                     ETKARVIKPDGRIVEFNSQNIREIVDAESGDNYRIFAIDGIEKGDDLEYIIIRRMEGA
                     NFGRAYFQFEFPVMEVSFELISPRNLKYAAKGYNGFPQPVADTTADGRNRLSCTARSI
                     SGLRSESYFYQNPHRARVEYKLEYNLARGKGSLLRWSDAAQRMYEMIYSDADPKIVKK
                     WIRTIDPGNGTGMEKASAVEDYIKRNIHIDDGDNPEYHDLEYVRTRKVTNEMGVVRLY
                     ANILRALDIRHELVLTSERDNIRFDPDFHSWNYLRKYLIYLSDEKIYIDPANDLYRVG
                     CIPGELTATYGLFISVVKIGDFESALGKIRFIEPAAYSENYDNMYIEISSDPVTNVST
                     ISATRGLKGLSGGYIYQIYKSIDEEKKQELLRSVMESKATNPDYKTLTTLERSDIEFM
                     RDAEFIIFSDFTTSSLLEMAGDKLLLNFGEIIGPQVELYDQENKERSAESPFNRWYLR
                     RIVFKIPDGYRIVNPEAAEMNITGIAGGETAFGFVSEWTCSGNIYTVDIDEYYKKVFV
                     EPAEFSGFRDVINAAANFNKVVLVLEKN"
     gene            complement(3564..5513)
                     /locus_tag="PLA17_06370"
     CDS             complement(3564..5513)
                     /locus_tag="PLA17_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPK85076.1"
                     /translation="MRKKLLVLLAALPLVLNLSAQQSTIRAERAAIMRDYNAVISDLE
                     HVYEFDIVKDSLRVIQHYSKKTEMLTDFTRLFADDKIYFDSFSTVSDLEAFTMVPGKK
                     GFEKIPVTLFNELNDPSGGIFYDDSKSLSFVYPSLKKGASTTLQYNLVHHNPRFLNKI
                     AFQSYLPILNARFTVKAHKDIRLEFRFFNCDDGFLKQSVYSRGKYNYHVWETHDVLPY
                     RYLVSDSYSILYDSPHVCVSVEEVKLRDTTLRYFSTVADLYRFYYSLLPSEEEAPSPP
                     LAELVESITGNKSRHDKVKAIYYWVQDNIKYVAYSEGYQGYIPATAEEVFNRRFGDCK
                     GQSSLIRKMIQIAGLKAWYAWVGTRKLPYTYSELPLPAVDNHMVVAYNEEDSLYILDG
                     TFRFIDFGMYPFSIQGKEVLVGVDSDNYFIFKVPVSGEEESIVYDSVNIDLTGDMITG
                     KGFRSHSGFNRYELAHAMEGVKPADYNRRVTSLFTKGNNKFSVDSCTFHSLYQHGVPA
                     SVSYSFNIADYARMVNNQVYINLNLDRSFSESKIDTTTRYAPVVNDFYYTEHQITRLR
                     IPEGYEISYLPDNDVIENEIISAYFRYSVDDGYITLDKKIKYKFLLLQSDRIKLWNST
                     IDQLNGNYRYSVALNKPETNSIIKK"
     gene            complement(5515..8784)
                     /locus_tag="PLA17_06375"
     CDS             complement(5515..8784)
                     /locus_tag="PLA17_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK85077.1"
                     /translation="MRKYHLFNLSVVVCCFSLTISAQKQLSDSLIRAGVRLYDEGKYN
                     EAISMYRQVDENDSNYVWMLGELSMTFLQTQDYDSVIYYASAGLENPSPYRQILMRNM
                     GTAYLAAGNREKSVEVYNDAIRLYPYSYLLHFNQGMTYMEMEDYRSAMNSLQEAIRCN
                     PFHASSHMRLGMLMARQEQYSRALLSLETFLALEPGTNRSNAVLVFIENLSSGHLDTT
                     YGDFIEPFAENDLFEELDGLIKSRIALNKKYKGAIKFNANLVKQTTLLMEMLPTEDQA
                     GDFWAETYLPFFRALKQGSFVEPFLYTILTSSGQEEVSQWISKNKKSLESFYQTGRYL
                     TSIRKSRLVTVDGKQQMLATSFDENGNLSSLGNSNSSGAEHGFWQYYFPNGELKAEGS
                     LSNGKKVGKWKYYTSDGLLESVENYTDGTINGEYLTYHPTGKLNITGNFSNGMVDGKV
                     IWRNIFGIPSRSLTFRNDTLHGEARTYYDSSQLMEAYENQNNQITGKHITYYASGQER
                     IIAYYNSGSLDGEYIEYHPNGRVSVRGTYKDGREEGEWTYWYSNGRVRLVSNYIDGVN
                     TGTVRTYYMNGLPESESNYNSSGKQDGISVTWDHLGRKVLEELYRDGILVRITTGFGS
                     PDEPVVCGSDDGTFSYRMYTFDGRLRTEGSYEKGVQTGEIRFYYKNGNLKQKFNLKNG
                     KYEGEVITCHPNGKTESVMHYKDGLLEGKYLEYGLDGTVIKEGSYLNNNMNNYWRNNL
                     ADGTSETSVYYINGLLNGWYTTYSVDGKIRSRDRFTDGRLVRKCQYDPDGNIVIDVDF
                     KETNAYQIKSMPDIVLADVVMKGGMHNGPLTWYNPDGKPSARMNFLADNQNGEYMQYF
                     PDGKPMAKGYYLNDNREGEWITWNEDGSIDIREHYFNNMKDSVWTYFYENGKVRMTET
                     YFNDMLDGECNQYAPDGELMIRLIYSEDELIGYQYMKGGKLTDIIALTKGDQPVIAWY
                     NNGKKSYEKHFRDFVAEGDQVKYYPDGKVMQRKQYRDGLLEGVQELFYPDGTPEAVYN
                     CSKGMFEGEYVKYRNSGKVKEKMQFLHDQAHGNRYLYDTAGNPMPGEKYWSGSFTGFI
                     R"
     gene            8945..9631
                     /locus_tag="PLA17_06380"
     CDS             8945..9631
                     /locus_tag="PLA17_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK85078.1"
                     /translation="MSKKKKETPRKGIENVEQTLTRTEQFLEQNYKPLLYVLAGAVLL
                     VGIAWLLRMYISKKNEEALSQMYMAEQYFGQDSLRLALNGDGNNLGFIDIAKEYRSTR
                     SGKLANFYAGACLMHLGQYEEAIGYLTKYKQNDEVIAPQAKGLIGDAHVELGDMAAGI
                     KNYLEAADMAENAFLTPIYLMKAGMLYENEGKWSDALKLYERVQDKYPESNEGRSIDK
                     YIARVKLHIN"
     gene            9635..10123
                     /gene="ribH"
                     /locus_tag="PLA17_06385"
     CDS             9635..10123
                     /gene="ribH"
                     /locus_tag="PLA17_06385"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPK85079.1"
                     /translation="MATRDLSYYDPSGVPDASEMRFGIVVSDWNSEVTHSLLNGAVKT
                     LKRHGVTDDNIIVRHVPGTFELTLGSQWMAEYEDLDGVICLGCVIQGETPHFTYICQG
                     VTRGITQLNLDYNIPFIFGVLTTLNLEQALDRAGGKHGNKGDEAAVTAIRMAALQRQM
                     ED"
     gene            complement(10344..>11061)
                     /locus_tag="PLA17_06390"
     CDS             complement(10344..>11061)
                     /locus_tag="PLA17_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPK85080.1"
                     /translation="KGESHAGRKIAALARSREIIRETIISAAMDEMRWDRETLMSGRG
                     VIIIDVLERYARELLEYDGVNPDMTLLHLEEDFSGAHTVVCGGEEVRITLGGRVDRID
                     LTGGAVRVVDYKTGAPKREVFTAEGLFDEEKEKRNDAFLQALLYCTLIEKSHPGKMVL
                     PAIYWIQQLSSDEFSPYAPVTGLKGPESAISEWGSFMNEYREHLNLALNNIFSADEDY
                     RMTAFRRRCTFCPYRTLCRR"
BASE COUNT         2740 a         2764 c         2401 g         3156 t
ORIGIN      
        1 tatcgtgaga tggatgaccc tttcggtcac gcatggaaga gacatctgtt accgggcaga
       61 gtcatgctga ggattcatgc tgcccggctc cttcaatgca gcactatctc cctgaccact
      121 tcctctccag ccctgctgtt tatccggctc ttcagcgaat cacgcagcat catcagctca
      181 ttcctcagaa ccgatgatgt aaggtatatg tgaagcacgc catccttcag gtatattctt
      241 ttggtccgtg ccgtaataac tttaccggca atatcttccc atatattcag cacagcagca
      301 tccttcagcc ctttttcaag ccggtactcc tttatcagtt ctccgagagc ctctcccagg
      361 gtgatggtct tgttccttcg catctcttac ttctctccgt tggtgatctc ctcctcgata
      421 ccctttttcc cgatacggta aagccggaag tcaaccccgg tattgtcaag aatccggtga
      481 atcctgtcct gctgggtgtc agtgataaaa acctgtccga actcccctga tcctgccagt
      541 ctgatgatct cctcaacacg gttctggtca aacttgtcaa agatatcgtc aaggagcagt
      601 gcaggtgcaa atcctatcat cctggtcatc aggccgaatt tcgccagctt gagggccacg
      661 atgaatgact tctgctggcc ctggctggca tttgtcctgg cggaatggcc atcaatctca
      721 aagaccaggt catcacggtg aattcccatt gtagtatgct gcattgcata gtctctctcc
      781 cgtgagtcgg caagctgttg ccggaaatca ccgctgctga ggtgtgaacg gtatctgatt
      841 gatacttttt cagcgcctcc ggatattctt tcgtagtaag agcgaaagag aggcagcagg
      901 ttttcggtaa ggctgcgtcg tgcattatag atcattccgg cctcaggaac catctgttcg
      961 tcatagatct caagcagtcc cgactgattg gcgctgtcgt ccctgagtat cctgttgcgc
     1021 tgcatcagag ccttgttgta tcgcatgtgt gcttcgaggt aggcagggtc atactgactt
     1081 atgatcaggt tgaggaaacg gcggcgctcc tcactgccac cggtgatgag ggtggtgtca
     1141 gacggggaga gcatcaccac agggtatttt cccacatggt cagacatcct ctggtactcc
     1201 ctgccattga gcttaaatac cttctgtttc gtcttctgaa acgcgcagaa gagatcgtca
     1261 ctgttatcgt cggtgacgaa tgttcccctt atcatgaaat aatcgtctcc atgcctgatg
     1321 ctgagggtgt cagaattgta gaagaaactc tttgtaagtg acaggtaatg gatggcgtca
     1381 aggatattgg ttttcccgac accgttattt cccacaaagc agttgaaccc cggagaaaat
     1441 tcgagggaaa cctcttcata attcttgaaa ttggtcagtt caagccttgc caggtgcatc
     1501 attccaatac gctcttacag gaggctcaaa gttacgaaaa gaaaaaggcg atgcaatccg
     1561 gcaaatccat taagtcagct ctccggtaaa taaaagcggc ggaccaggct aattcttctc
     1621 aagtacgagt accaccttgt taaaatttgc agcggcattg atcacatccc gaaatccgct
     1681 gaattcagca ggctccacaa atactttttt gtaatactcg tcaatatcaa cagtatatat
     1741 gttcccggaa caggtccatt cagatacgaa tccgaatgct gtttctccgc cggcaatacc
     1801 tgttatattc atctcagcag cttccggatt aacaatcctg tatccgtcag gtatcttaaa
     1861 gacaatgcgc ctcaagtacc acctgttgaa aggtgactct gcacttcgct ccttgttctc
     1921 ctgatcataa agctccacct gagggccgat gatctccccg aagttcagaa gcagcttgtc
     1981 acctgccatc tcaagcaagg atgatgtggt gaaatcactg aatataatga actcggcatc
     2041 cctcatgaat tctatatcgc ttctttcaag tgttgtaaga gttttataat caggatttgt
     2101 agcttttgat tccatgacag acctaagcaa ctcctgcttc ttttcctcat ctatactctt
     2161 gtatatctga taaatatatc ctccgctcaa tcccttcaga cctctcgtgg cagagatcgt
     2221 ggaaacattt gtgaccggat cagaggagat ctcaatatac atgttgtcat aattctcact
     2281 gtatgcagcc ggctcaatga accgtatctt tcccagtgct gattcaaagt cacctatctt
     2341 caccactgat atgaacagtc cgtaagtggc tgtcagttca cccggtatgc atcctaccct
     2401 gtacaggtca tttgccgggt caatgtatat cttttcatca gagaggtata tcaggtactt
     2461 tctgagatag ttccaggagt ggaagtctgg atcgaacctg atgttgtcac gttcagaggt
     2521 cagtaccagt tcatgcctga tatcaagtgc gcggaggata tttgcataga gccgtaccac
     2581 acccatttcg tttgtgacct ttcttgttct gacatactca agatcgtggt attcagggtt
     2641 gtcgccatcg tcgatatgta tattcctctt gatataatcc tcaacggcag aggctttttc
     2701 catgccggta ccatttcccg gatcaatcgt cctgatccac tttttaacaa ttttgggatc
     2761 agcatctgaa taaatcattt catacatcct ctgggctgca tcgctccatc tcagcaggct
     2821 gcctttaccc cttgcaagat tatattcaag tttatattcc acccttgcgc gatgcggatt
     2881 ctggtaaaaa taactctcac tcctcagtcc gctgatgctc cgggcagtgc atgacagtct
     2941 gttcctcccg tcagccgttg tgtcagcaac aggttgcgga aaaccgttat aacctttggc
     3001 tgcatacttc aggttcctgg gagaaatcag ttcaaatgac acctccatga ccggaaattc
     3061 aaactggaag taagcccttc caaagtttgc cccttccatt ctccttatga tgatatactc
     3121 aagatcatct cccttctcaa taccgtcaat agcaaagatc ctgtagttat ctccgctttc
     3181 ggcatccact atttccctga tattctgact attgaactca actatccgtc cgtcaggttt
     3241 tatgaccctc gctttcgtct cctccatttc aatcacatcg gccattgata cggagagttt
     3301 attgaatccg tttatggcat catcagtatt aagccttatt ctccggtgga gggtatgaat
     3361 catttccagt tggccttcag gattatacag gtactcagtc ttttcattgt agaaaatacc
     3421 gactgcattt tcattttgat tttctgattc cagagagaag tagacaggct tctcctccca
     3481 tgttgtgcgg ttccatgaat cctgggcctg taaaacagca gacgttgcca gtaacagcag
     3541 tattaaaact gaaaatatcc tcatcatttt tttattattg aattagtttc aggtttattt
     3601 aaggccacag agtaccggta gtttccgttc agctggtcta tggttgaatt ccatagttta
     3661 atccggtcac tctgaagcaa gaggaatttg tatttgattt tcttatcaag agtgatataa
     3721 ccgtcatcta cggaataccg gaaataagcg ctgataatct cattttctat tacatcatta
     3781 tcaggcagat aactaatttc ataaccctca gggattctta aacgggtaat ctggtgctca
     3841 gtataataaa agtcatttac aaccggagca tacctggttg tggtgtctat tttgctttcg
     3901 gagaatgatc tgtcaaggtt caggttaata taaacctggt tattgaccat ccgggcatag
     3961 tcagcaatat tgaagctgta gcttacactt gccggcaccc cgtgttgata gaggctgtgg
     4021 aaggtgcaac tgtcaacgct gaacttgttg ttccccttgg taaagaggga agtgacacgc
     4081 ctgttatagt cagcaggttt gactccttcc attgcatggg ccagttcgta gcggttgaag
     4141 ccgctgtgtg aacggaatcc tttgccggta atcatatcac cggtgagatc tatgttgaca
     4201 gaatcataaa caatgctctc ttcctcaccc gagaccggca ccttgaaaat gaaataatta
     4261 tcgctgtcaa caccgacaag cacctccttt ccctggatgc tgaaaggata catcccaaaa
     4321 tctatgaacc ggaatgtgcc atcgagaatg tagagagaat cttcctcatt gtatgccacc
     4381 accatgtggt tgtcaaccgc cggcagcggc aattcgctgt aggtgtaggg cagtttacgt
     4441 gttcccaccc aggcatacca ggctttcagt ccggcaatct gaatcatctt cctgataagt
     4501 gaactctggc ctttgcaatc accgaagcgc ctgttgaaga cctcctccgc cgtggcagga
     4561 atgtatccct ggtagccctc agagtaagca acatatttga tattgtcctg tacccagtaa
     4621 tagattgctt ttaccttatc atgcctgctt ttgttcccgg taatgctttc taccagttcc
     4681 gccagcggcg gggatggagc ctcttcctct gaaggaagca ggctgtaata aaacctgtac
     4741 agatcagcta ccgttgaaaa gtatctgaga gtggtatccc tgagtttaac ctcctcaacc
     4801 gagacgcaaa catgagggct gtcataaagg atgctatagg aatcactgac gagatatctg
     4861 tagggcagca catcgtgtgt ttcccataca tggtagttat acttccccct ggaataaacc
     4921 gattgtttta aaaagccgtc gtcacagttg aaaaaccgga attccagcct tatatccttg
     4981 tgcgccttta cggtgaacct cgcattcagg atcggcagat aagattgaaa tgctatcttg
     5041 ttaaggaagc ggggattgtg gtgtaccaga ttatactgaa gcgttgttga cgcccccttt
     5101 ttcaatgaag gataaacaaa actcaggctt tttgagtcgt cataaaagat gcctccggag
     5161 gggtcattca attcgttgaa cagtgtcacg ggaatctttt caaatccctt tttgccagga
     5221 accatggtaa atgcctccag gtcactgacg gtagaaaatg agtcgaaata gatcttgtca
     5281 tccgcaaaaa gcctggtaaa gtcagtcagc atttctgttt tcttgctgta gtgctggatg
     5341 actctcaggc tgtcctttac gatgtcgaat tcgtaaacat gctccagatc actgataacc
     5401 gcattgtaat ctctcatgat ggctgcccgt tcagccctga ttgtactctg ctgggcagag
     5461 aggttcaaga ccagaggcag ggcagccaac agcaccaata acttttttct catctcaccg
     5521 gatgaaacct gtgaaactgc cactccagta cttttcgccc ggcatggggt ttcctgcagt
     5581 gtcatacagg tacctgttcc catgcgcctg atcatggaga aactgcatct tttcctttac
     5641 tttccccgag ttccggtatt tgacatattc tccttcaaac atgcctttac tgcaattata
     5701 aacagcctca ggagtgccat caggataaaa tagttcctgc acaccttcaa gcaagccatc
     5761 cctgtattgt ttccgttgca ttacttttcc gtcagggtag tatttgacct gatcaccctc
     5821 ggcaacaaaa tcgcgaaaat gtttttcgta actttttttc ccgttgttgt accaggcaat
     5881 aaccggctga tcgccctttg tcagagcaat aatgtcggtc agtttccctc ccttcatgta
     5941 ctggtaacca ataagctcat cctcgctata gatcagcctt atcataagtt caccatccgg
     6001 agcatactga ttgcactctc cgtcaagcat gtcattgaaa taagtttccg tcattcttac
     6061 ttttccattc tcgtagaaat aagtccagac cgaatccttc atgttgttga aataatgctc
     6121 cctgatgtca atgctgccgt cctcattcca ggtaatccac tccccttccc tgttatcgtt
     6181 caggtagtaa cccttcgcca ttggcttacc gtcagggaaa tattgcatgt actcaccgtt
     6241 ctgattgtct gcaaggaaat tcatcctcgc ggagggtttc ccgtcaggat tataccaggt
     6301 taatggtccg ttatgcatcc ctcccttcat taccacatcg gcaagaacta tgtcaggcat
     6361 gcttttaatc tggtaggcgt ttgtctcttt gaagtcaaca tcaattacaa tatttccatc
     6421 aggatcatac tggcactttc tgaccagcct gccgtcagta aacctgtccc tgcttctgat
     6481 tttaccgtca actgaataag ttgtatacca accatttaat aaaccattta tgtaatatac
     6541 tgatgtttca ctggtaccat cagcaagatt gttgcgccaa tagttgttca tgttgttatt
     6601 caggtaagac ccttccttta taacagtacc gtcaagcccg tactcaaggt atttgccttc
     6661 cagcaaacca tccttgtaat gcatgacaga ctcggtcttt ccgttcggat gacaggtgat
     6721 gacctctcct tcatattttc cattcttcag gttgaatttc tgcttcaggt ttccattctt
     6781 gtaatagaat ctgatttcac ctgtctggac gcctttctca tatgagccct cagtcctcag
     6841 tctcccgtca aatgtgtaca tacggtagct gaatgtgccg tcatcactac cgcagactac
     6901 cggttcatca gggctgccaa agccggtagt tatcctgaca aggattccgt cacggtacaa
     6961 ctcttccaat acctttctgc caaggtgatc ccatgtcact gaaatcccgt cctgcttgcc
     7021 cgagctgttg taattcgatt ctgattcagg caaaccgttc atgtaatagg ttctgacagt
     7081 gcctgtattg acgccgtcga tgtagttact tacaagtcta accctgccgt ttgaatacca
     7141 gtatgtccat tccccctcct ccctgccgtc tttgtaggtt cccctgacac tcactcttcc
     7201 attgggatga tactcaatat attctccgtc aagtgaccct gagttgtaat aggcaattat
     7261 tctttcctgt ccggaagcgt aatatgtgat atgcttgcca gtgatctggt tattttgatt
     7321 ttcatatgct tccattagct gggatgagtc gtaataggtt ctggcctcgc catggagcgt
     7381 gtcatttctg aaggtgagtg atcttgacgg tatcccgaag atgttccgcc agatgacctt
     7441 gccgtcaacc atgccgttgg aaaaatttcc tgtaatatta agtttacctg tcgggtgata
     7501 agttaaatac tcaccgttga ttgtgccatc cgtgtaattc tcaactgatt caaggagtcc
     7561 gtcggaagtg tagtatttcc attttccaac ctttttgccg ttggagagcg acccttcagc
     7621 ctttagttcg ccgttgggga aataatattg ccagaagcca tgttctgcac cggaagaatt
     7681 actgtttccg agggaactga ggtttccgtt ttcgtcaaag gatgttgcaa gcatttgttg
     7741 cttaccgtca accgtaacca gcctgctttt ccttatgctg gtaagatacc tgcctgtctg
     7801 gtaaaatgat tcgagtgact tcttgttctt gctgatccat tggcttacct cctcctggcc
     7861 tgatgaggta agaattgtgt ataggaatgg ctcaacaaaa ctgccctgtt ttagtgcccg
     7921 gaaaaaaggc aggtaagttt ctgcccagaa atctccggcc tgatcttctg taggcagcat
     7981 ctccatgaga agtgtagtct gctttaccag gttggcattg aattttattg cccccttata
     8041 ttttttattg agagctatcc ttgacttaat gagcccgtca agttcctcaa acagatcatt
     8101 ttcggcaaaa ggctcaataa agtcaccata ggttgtgtca agatgccccg aggagagatt
     8161 ttcaataaag accagtactg cattgctgcg gttagttccg ggctcaaggg caaggaatgt
     8221 ctcaagtgac agaagcgcac ggctgtactg ttcctgcctt gccatcagca tcccgagcct
     8281 catatggctg ctggcatgga acggattgca tctgatggcc tcctgaaggc tgttcatggc
     8341 tgacctgtaa tcttccatct ccatataggt catgccctgg ttgaagtgaa gcagatacga
     8401 ataaggataa agtcttatgg cgtcattgta cacctctacc gacttttctc tattgccggc
     8461 cgccaggtat gcagtgccca tgttacgcat aagaatttgc ctgtacggcg atggattctc
     8521 cagtcctgca ctggcatagt aaatgacgct gtcataatcc tgcgtctgca gaaaggtcat
     8581 actcagctct ccaagcatcc agacataatt ggaatcgttc tcgtcgacct gtctgtacat
     8641 actgatggct tcattgtatt tcccttcatc atacaatctt acgcctgccc tgataaggga
     8701 gtcagataac tgcttctgtg cactgattgt cagactaaaa cagcagacaa ccacagatag
     8761 attaaacaag tgatactttc tcatagccat gacttatatt gacagtttag cattaacaaa
     8821 cttatgcttt cctcttctta tctcctaata ctcaacgaca aatttttaaa tttatatttt
     8881 taaataatgc cagaaatact acttttgcga agttattttt tgacctgtaa cagagaacat
     8941 tgaaatgagc aagaaaaaga aggagacccc cagaaaaggg atcgaaaacg ttgagcagac
     9001 actgacaaga acagaacaat tccttgaaca gaattacaaa cccctgttat atgttcttgc
     9061 cggagcagtg ctgctggtgg gaatagcatg gcttctcagg atgtatatca gcaaaaagaa
     9121 tgaagaggct ctgagccaga tgtacatggc agagcagtat ttcggacagg actcactcag
     9181 gctggctctc aacggtgacg gcaacaatct tggctttatc gatatagcga aggaatacag
     9241 gagcaccagg tcaggcaagc ttgccaactt ttatgcagga gcatgcctta tgcatctcgg
     9301 tcagtatgaa gaggcaatag gctacctgac caaatacaaa cagaatgatg aggttatcgc
     9361 gccacaggcg aagggtctga ttggcgatgc gcatgttgag cttggcgaca tggctgcagg
     9421 aataaagaat taccttgagg ccgccgacat ggctgaaaac gcgttcctca cccccatcta
     9481 cctcatgaag gcgggaatgc tctacgagaa tgaaggtaaa tggagcgatg ccctcaaact
     9541 atacgagcgt gtgcaggata agtacccgga aagcaacgaa ggccggagca tcgacaagta
     9601 tattgccagg gtcaaactgc acatcaactg acagatggca acccgcgatc tttcatatta
     9661 tgatccctca ggcgttcccg atgcctcgga gatgaggttt ggtatcgtgg tgtccgactg
     9721 gaacagcgag gtaacccatt cacttcttaa cggtgccgtt aaaacactga agcgccacgg
     9781 tgtaacggac gacaatatca ttgtcaggca tgtcccgggc acgttcgagc tgactctcgg
     9841 atcgcagtgg atggctgagt atgaagatct tgacggtgta atttgcctgg gatgtgtcat
     9901 tcagggtgag acacctcatt ttacatatat ctgccagggg gtgacccggg gtatcaccca
     9961 gcttaacctt gactataaca tacccttcat cttcggcgtc ctgacaacgc tgaacctgga
    10021 gcaggctctt gacagggccg ggggcaagca tggcaacaag ggcgatgagg cagctgtgac
    10081 agccatcagg atggctgcac tacagcggca gatggaggat taacctgctg tttactgccc
    10141 cttaatctgc cggtaccgct aatcacggta cacctgatca gctataggat taattatgca
    10201 cccgcctcat tcagtgcaaa tcaggagcag aggtcaggat ggctattcat tgtatctatc
    10261 tcctgcagag caggctccaa ggcagaggtc aggatgccgc tatctcctgc agagcaggct
    10321 ccggggcaga ggtcaggatg ccgctatctc ctgcagagcg tcctgtacgg gcaaaaggtg
    10381 cagcgccggc ggaatgctgt catccggtaa tcctcatcgg ccgagaagat attattcagt
    10441 gccaggttca gatgttccct gtattcattc ataaagctgc cccattcaga gatggcactc
    10501 tccggccctt tcagtcctgt aaccggtgcg tatggtgaaa actcatcaga tgacagctgc
    10561 tgaatccagt aaattgccgg aagcaccatc tttccggggt ggcttttctc aatcagtgta
    10621 cagtaaagca gcgcctgcaa aaaggcatca ttgcgttttt ctttttcctc atcgaacagt
    10681 ccctctgccg taaacacttc gcgctttggc gcaccggtct tgtaatctac aacccttaca
    10741 gcccctccgg tgagatctat cctgtctacc ctgcctccca gggttattct cacttcttct
    10801 ccaccgcaga caactgtatg cgcacctgag aaatcttcct caaggtgaag aagagtcata
    10861 tccggattaa caccatcata ttcaagcagt tccctggcat atcgctcaag cacgtcgatt
    10921 attatcactc ccctgcctga catcagagtc tccctgtccc acctcatctc atccatggca
    10981 gcggaaatga ttgtctctct tataatctca cgtgaccggg ccagtgcggc aatcttacga
    11041 ccggcatggc tttctccttt c
//
LOCUS       DAOPBK010000086        10850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_5916_length_10850_cov_4.403705, whole genome shotgun sequence.
ACCESSION   DAOPBK010000086 DAOPBK010000000
VERSION     DAOPBK010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_10850_cov_4.403705"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2532)
                     /gene="leuS"
                     /locus_tag="PLA17_06395"
     CDS             complement(<1..2532)
                     /gene="leuS"
                     /locus_tag="PLA17_06395"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPK85081.1"
                     /translation="MKDYNFSEIEKKWQDYWEKNKTFKTTDDLSNPNKCYILDMYPYP
                     SGSGLHVGHPEGYTATDIVARYKRMKGYNVLHPMGWDAFGLPAEQYAIQTGTHPAVTT
                     NKNCDIFRRQIKALGLSYDWDREINTTDPKYYKWTQWIFLKLYNTWYDHEANRGRDIN
                     EMPIPLDVLKAGDEARLKYINSRRLAYYDEAQIWYCPSCRIVCANEEVLTDGSHEKCG
                     NRVVRKKLKQWMLRIPYYAERLLSGLDSLDWPEGIKDMQRNWIGRSTGAEVDFKIDGT
                     SERITVYTTRPDTLFGATYMVLSPEHPLVEKITNPEMVSQVKLYVEAASLKSDLDRTD
                     LARDKTGVFTGTYAVNPVNNKKIPVWVADYVLMGYGTGAIMAVPAHDERDFEFAKKFG
                     IDIICILDPAVTDQEQRERIIAGNEYWTGDGSYINSANKELNISLDGLNVEEGIATIT
                     AWLEGRNLGIHRVNYKLRDWLFSRQRYWGEPFPVIHWEDGTISLLDEEDLPLTLPELE
                     VYEPGENGESPLSNATDWLWVTDQNGRRGKRETNTMPQWAGSCWYYLRFIDPHNDKAI
                     FDPEKERYWMPVDLYIGGAEHAVLHLLYARFWHKVLFDLGVVSTDEPFMKLFNQGMIL
                     AFAYETVTGAKISSDQITEKEGKFFITETGEEVRQIVAKMSKSLKNVINPDDVVRNYG
                     ADTLRLFEMFLGPLEATKPWDEKGIRGPHNFLSRTYRFIANSENITEGDEDTEVLKAL
                     HKTIKKVTEDIEGLRFNTAISAMMIFMNIAQKKEKITRTTASLFIRLLSPFAPHLAEE
                     LWQATGHKESLTYEPWPVAEEKYLKEDLFECPVSVNGKMRFKIELP"
     gene            2759..2831
                     /locus_tag="PLA17_06400"
     tRNA            2759..2831
                     /locus_tag="PLA17_06400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2792..2794,aa:Arg,seq:ccg)
     gene            complement(3097..4428)
                     /locus_tag="PLA17_06405"
     CDS             complement(3097..4428)
                     /locus_tag="PLA17_06405"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPK85082.1"
                     /translation="MKSKKRVVIAGGGFGGLQLARRLSKKDFDVILVDSQNHHQFQPL
                     FYQVASARLEPATISFPFRKIFQRSTNLKFLLARAQEIIPDQNEIVTSTGSYSYDYLV
                     IATGCQTNYFGRDDIMQHALPMKTTMDAIEIRNRILLGFESLFAAREGNTEELLNQDA
                     RLQIHAKAAREGNTEELLNIVIVGAGPTGVELAGAFAEMKRFILPRDYPGFDFSALKI
                     ILVGGSTSTLNNMSDKAKKASMKYLKELGVDVMTGVFVTGYDGSTVILNNGDTIKCKN
                     LIWAAGVTGNLINGIRPESITRSQRYIVDRQNRIKGYSNIFAIGDIAYMETPLYPNGH
                     PQVANVAINQGKLLARNLKALATGNEMDSYEYRDLGSMATIGKHKAVVDLPFMKFKGY
                     FAWLIWMFLHLMLILSVKNKLMIFITWAWNYLTRDSSLRLIFNPGYPDKAR"
     gene            complement(4461..6101)
                     /locus_tag="PLA17_06410"
     CDS             complement(4461..6101)
                     /locus_tag="PLA17_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPK85083.1"
                     /translation="MANNFFAKKSVEELIAQTEEGDHKLKRALGSFDLVLLGIGAIIG
                     TGIFVLTGVGAALHAGPAITLSFGVSAIACVFAALCYAEFASMIPVSGSAYTYAYATM
                     GELLAWIIGWDLILEYAVCSITVAIGWSGYLVNLLAGLGIIIPAWMCAPPFNLPALII
                     VGIITTLLVIGIKESAKVNSIIVFIKLAVILFFIILGLFFIRPENWKPFMPFGWTGVM
                     TGAAIVFFSYIGFDAVSTTAEETKNPQRNLPIGIIVSLVICSTLYIIVALVLTGIIPV
                     MDYADNQSFLAAPIAFALNVINQDWASGIISVGAVMGITSVLLVMMMGQPRVFFAMSR
                     DRLFPEKISKVHPRFRTPYRTTIITGIAVGLIALMIPIGTAAELANIGTLFAFAIVSA
                     SVLILRKTNPALKRSFRVPLVWIISPLGVLFSFYLMFSLPVLTWLRFLAWLDIGLLIY
                     YFYGRTHSRISDKVHRDKRFKLNDMLEFFGIFALVNGILFGLLSLLAITGITSLKSWD
                     KISFEPHHSLIVCVILIAAGAVLFLTGRILNRGENRKG"
     gene            complement(6117..7559)
                     /locus_tag="PLA17_06415"
     CDS             complement(6117..7559)
                     /locus_tag="PLA17_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPK85084.1"
                     /translation="MANKLFRTKSLQQMMATASDSEHGLKRALSATNLIFIGIGCIIG
                     TGIFVITGTAAAQYAGPALSISFLISAIVCMFVALCYTEFASMIPIAGSAYSYAYTTV
                     GEFLAWFIGWNLVLEYIFGASLVAVGWSGYVVSFLKDFGIVIPPGLCNAPLDYHDGWI
                     TTGAMINLPSIFIVALITTVLVFGIKESVRFNNIIVVIKIAVILLFIGFGLSYINPDN
                     YVPFIPENTGVHGEFGWSGIFRAAGIIFLAFVGFDAVSTVAQEAKNPQRDMAKGILGS
                     LIIVTILYIAVSLVLTGMVNYKYLNVPDPIAVGIDSAGPGLFWLRPIIKIGAIAGMSS
                     VILVLITGMPRILYSMSNDGLLPPVLGRVHKKYRTPHITTIIVGSIIAVISGLFPIDL
                     IGELVSIGTLLAFAMVCISIMILRVRRPDMNRPFRTPVVYFVGTAGAAGCFYLMFSLP
                     GSTWFRLGVWTVIGFLIYFLYGRKHSKLNV"
     gene            complement(7863..8342)
                     /locus_tag="PLA17_06420"
     CDS             complement(7863..8342)
                     /locus_tag="PLA17_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPK85085.1"
                     /translation="MARFVKTGKKTIKAISTSSLPDIVFMILFFFMAVTTMRETDVMV
                     TVRIPEATEGKVLEKKSLASFIYIGEPLESFQALYGKEPKIQLNDSFKELADIQDFIA
                     QEREQLNEADKAKMLVVLKIDRDTKMGIVTDVKQALRRASALKIMYANTNVDKITSF"
     gene            complement(8347..8922)
                     /locus_tag="PLA17_06425"
     CDS             complement(8347..8922)
                     /locus_tag="PLA17_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPK85086.1"
                     /translation="MARRQVPEINAGSMADIAFLLLIFFLAATTMDSDTGLMRQLPAI
                     PDPNVPQDVNKINDRNILEVKVNKDNLLLVEGQLTEFDQLREIAHNFILNPNDEPSMP
                     EREVVYLPFFGNVAVTKAVISLQNDIGTKYGVYLAVQNELIAARAKLRDDVARQEFGI
                     PYDRLDEDRKSAVDDYYKVPISEAEPKRIGD"
     gene            complement(8963..9424)
                     /locus_tag="PLA17_06430"
     CDS             complement(8963..9424)
                     /locus_tag="PLA17_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85087.1"
                     /translation="MSEKITRLTLILLWVLMGITVIFAILFYVGGVVPGTEGTRYHEP
                     KVTNSFIVYAYVLLAVTVLTLLFFAVRSYVINPKGLKGLLIALGVFAVLIGISALLAD
                     NTVLDLPHYKGKDNVPGTLFMTDIGLYVAYFLLAGAFLTIIFSVISQYFRK"
     gene            complement(9431..10237)
                     /locus_tag="PLA17_06435"
     CDS             complement(9431..10237)
                     /locus_tag="PLA17_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPK85088.1"
                     /translation="MKRVLVFMAFAALLLIGTAQTSQAQTDQTATNQQTTEQAQAATT
                     RQLDAQATTPAQGETVLHKEIKRLFIEGGAGFMGVILLCLIFGLAIAIERIIYLNLAT
                     TNTDKLLAKVNKALNEEGIDAAKEICRNTRGPVASIFYQGFDRAHEGVEMAEKSVISY
                     GGVQMGLLEKNLSWISLFIAIAPMLGFLGTVIGMIQAFDAIEQAGTVSATIVAGGIKV
                     ALITTVFGLIVAIILQVFYNYLLSKIDGISNQMEDSSITLIDMLAKYNQK"
     gene            complement(10482..10784)
                     /locus_tag="PLA17_06440"
     CDS             complement(10482..10784)
                     /locus_tag="PLA17_06440"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPK85089.1"
                     /translation="MKFTVEYNPDFFNDIVQAVDWYNEKQAGLGNRLLNNIKKQTTKL
                     STTALHFAVKYDDIRCMCIEKFPYLVHYRVNEQTKTVKVEALFHTSRDPKIWDERM"
BASE COUNT         2705 a         2589 c         2675 g         2881 t
ORIGIN      
        1 aggcagctct atcttaaacc gcatcttccc gttgaccgag acagggcatt cgaacagatc
       61 ctctttaagg tatttttctt cggccacggg ccatggctca taggtcaatg attccttgtg
      121 cccggtggcc tgccacagct cttccgcgag gtgaggtgca aacggagaga ggagcctgat
      181 gaagagtgaa gctgtggtac gggtgatctt ctcctttttc tgtgcgatgt tcatgaagat
      241 catcattgcc gagatggccg tgttgaacct gaggccctct atatcttcag tcaccttttt
      301 aattgttttg tgaagggcct tgagaacctc agtgtcttca tcgccttcag tgatgttctc
      361 actgttggct atgaagcgat aagttcttga caggaagtta tgcggacccc tgataccctt
      421 ctcgtcccat ggctttgttg cctccagcgg gccgaggaac atttcaaaca ggcgaagcgt
      481 gtctgcgccg tagttcctga ccacatcgtc agggttgatg acattcttaa ggctctttga
      541 catcttggcc acaatctgcc tgacctcttc accggtttcc gtgataaaga atttgccctc
      601 tttctcggtg atctggtctg atgatatttt ggctcccgta acagtttcat aggcaaatgc
      661 aagtatcatt ccctggttaa agagcttcat gaagggctca tcggttgata ccaccccgag
      721 gtcaaaaagc accttgtgcc agaagcgggc ataaaggagg tggagaacgg catgctcagc
      781 tcctcctatg taaaggtcaa ccggcatcca gtatctctcc ttctcagggt cgaatatggc
      841 tttgtcattg tgcgggtcta tgaatctcag gtaataccag catgatccgg cccactgtgg
      901 catggtattg gtctcgcgct tgccgcgacg cccgttctgg tctgtgaccc atagccagtc
      961 ggttgcattg gagagaggcg attcaccgtt ttcacccggc tcgtagacct cgagctcagg
     1021 gagggtcagg ggcagatcct cctcgtcaag aaggctgatg gttccatctt cccagtggat
     1081 aacggggaag ggttcacccc agtagcgctg cctgctgaag agccagtcgc gcagcttgta
     1141 gttgacacga tggattccca ggttcctgcc ctcaagccat gccgtgatgg tggcaatccc
     1201 ctcttcaaca ttcagtccgt caagactgat attgagctcc ttgtttgccg agttgatata
     1261 actgccgtca ccggtccagt actcgttgcc ggcgattatc ctctcacgct gctcctggtc
     1321 agtcacagcc gggtcaagga tacatattat atctatcccg aacttctttg caaattcaaa
     1381 gtcgcgctcg tcgtgagccg gcactgccat gatggcgcct gttccgtaac ccataagcac
     1441 gtagtcagcc acccataccg gtattttctt gttgttgaca gggttgacgg cgtatgttcc
     1501 ggtaaagaca ccggttttgt ccctggccag gtcagtgcgg tcaagatccg acttcaggga
     1561 ggctgcttcc acatataact tcacctgcga taccatctcc gggtttgtga tcttttccac
     1621 aagagggtgc tctggtgaga gtaccatata ggttgccccg aagagtgtat ccggacgggt
     1681 ggtatagact gttatccgtt cgctggtgcc gtcaatttta aagtcaacct cggcgccggt
     1741 ggagcgtcct atccagttac gctgcatatc cttgatcccc tctggccagt cgaggctgtc
     1801 aagtccgctg agtagtctct cggcatagta agggatacgg agcatccact gtttgagttt
     1861 cttgcgcacc accctgtttc cgcacttctc gtgcgaaccg tcggtcagca cctcttcgtt
     1921 ggcgcagaca atacggcatg aggggcagta ccatatctgt gcttcatcat aatatgccag
     1981 tctcctggaa ttgatgtatt tcagccttgc ctcatcacct gcctttagga cgtcaagggg
     2041 tatgggcatt tcatttatgt cacgtccccg gttggcttcg tgatcatacc aggtgttata
     2101 gagcttcagg aagatccact gtgtccactt gtaatatttc ggatcagtgg tatttatctc
     2161 gcggtcccag tcgtagctca gtcccagtgc ctttatctgg cggcggaaga tgtcacagtt
     2221 tttgttcgtg gtgacagccg ggtgggttcc ggtctggatg gcgtactgtt cggccggcag
     2281 gccgaaggca tcccatccca tgggatggag cacgttgtaa cccttcatgc gtttgtaccg
     2341 cgccacgatg tcagtggcag tataaccctc cggatggccc acgtgcagcc cggagccgga
     2401 aggatagggg tacatgtcaa ggatatagca cttattggga tttgacagat cgtcagtagt
     2461 tttaaaggtt ttattcttct cccagtaatc ctgccatttc ttctctattt ccgaaaaatt
     2521 gtaatccttc atctattgct tatgattagc gcctacgggt tgcgaaaata ggaaatcttt
     2581 gttaaaaatc ttcccggacg ggggacaggt cattgatatt tgcgtcagaa acagctgaaa
     2641 atgttgcctg caggcactct tttgctgcgt aggtgcagcg ggaggcaaca gatgtgccgt
     2701 caaatttgtg ctgtagtttt gaaaatcagt tgcagcggta tatttttgca ctctgaacgg
     2761 tcccgtagtt caatggatag aatggcagat tccggttctg ttggttgggc gttcgagtcg
     2821 ccccgggatc acaaaggaaa aacggcctcg acagccgttt tttgtattct ggcatcaggt
     2881 atcttaagat ttagggcgac gagaacgcga aggggaggag cgttcgaacc gaacgagctg
     2941 cgcgagtgag ctcgtgagcg gactgtgtgc ctgcatgtgt gtagcgaaca gtcgccccgg
     3001 gatcacaagg aaaaactgcc tgcctgcatg cgcgtggcga acagtcgccc cgggatcaca
     3061 aggcaggcag ataggcccgt cagggtattg ccagcactac cgggccttat ccggatatcc
     3121 gggattaaag ataagtctga gggatgaatc cctggtaaga tagttccatg cccaggtgat
     3181 gaatatcatc agcttgtttt tcacgctgag gataagcatg aggtgcagga acatccagat
     3241 cagccaggca aaataccctt tgaatttcat aaacggaaga tcgacaaccg ccttgtgctt
     3301 tcctatcgtg gccattgaac caagatcgcg gtactcatat gagtccattt cgttcccggt
     3361 ggccagagcc ttcagattcc gggcaaggag tttcccctgg ttgatggcca catttgctac
     3421 ctgcgggtga ccgttcggat aaagaggggt ttccatatat gctatatcgc ctatggcgaa
     3481 gatatttgag tagcctttaa tacggttctg acgatcaacg atgtaccgtt gtgaccgggt
     3541 tatgctttcc ggccttattc cattgatgag gttcccggtg acgccggctg cccagatcag
     3601 gtttttgcat tttattgtgt caccgttgtt aaggatcact gtactgccgt catagccggt
     3661 aacaaagact cctgtcatta catcaacacc cagctctttc aggtacttca ttgatgcctt
     3721 ttttgcctta tcactcatat tgttgagtgt gcttgtgctg ccccccacga gtataatctt
     3781 cagggctgaa aaatcgaaac cgggatagtc cctgggaagg ataaaccgct tcatttctgc
     3841 aaatgcacct gccagttcaa ctccggtggg tccggctccg acaatcacaa tattgagcag
     3901 ctcttctgta ttgccctccc ttgctgcttt tgcatgaatc tgcaagcggg catcctgatt
     3961 tagcagctct tctgtattgc cctcccttgc cgcaaagaga ctctcaaagc cgagcagtat
     4021 cctgttccgg atctcaattg catccatagt ggttttcatt ggcagggcat gctgcattat
     4081 gtcgtcacgg ccgaagtaat tcgtttggca tccggtagca atcaccaggt aatcataact
     4141 gtaactaccg gtggaggtta ctatttcatt ttggtcaggg atgatctcct gtgccctggc
     4201 aagcaggaat ttcaggttgg tcgagcgctg gaagattttt cggaagggaa atgatattgt
     4261 agcgggctca agcctggcgc tggcaacctg gtaaaataac ggctgaaact ggtggtgatt
     4321 ctgactgtca accagaatga catcgaaatc ttttttactg agtcttcggg caagttgaag
     4381 ccccccgaaa ccgcctccag ctattacaac tctctttttt gatttcatac cgtcattatt
     4441 ttaaacggta ctgtcaattc tcagcctttc ctgttttctc ccctgtttaa tattcttcct
     4501 gtaaggaaaa gaacagcacc tgcggcaata agaataacac atacaatgag tgaatgatga
     4561 ggttcaaagc tgatcttgtc ccatgacttg agtgatgtta tcccggtaat agccagaagt
     4621 gaaagcaggc caaaaagaat gccgttgaca agtgcgaaga ttccgaagaa ttcaagcatg
     4681 tcattcaatt tgaatctctt gtctctgtgt accttatctg aaatcctgct atgtgtgcgg
     4741 ccgtaaaaat aatagatcag caggccgata tcgagccagg caagaaacct cagccatgtt
     4801 agaacgggca ggctgaacat caggtaaaag ctgaagagca caccaagggg tgagataatc
     4861 cagacaagcg gcaccctgaa ggaacgcttg agggccgggt tagtcttcct caatataagt
     4921 acactggccg aaacgatggc aaatgcgaac agtgttccta tattggcaag ttccgcggct
     4981 gttccgatgg gtatcataag ggctataaga cccactgcaa tacccgtaat aattgtagtg
     5041 cgatagggtg tcctgaaacg ggggtgtacc ttcgagatct tctcaggaaa caggcggtca
     5101 cgtgacattg caaagaagac cctgggctgc cccatcatca ttacgagaag aacgcttgtt
     5161 atccccatga ctgctcccac ggagatgatt cctgatgccc agtcctgatt aatgacatta
     5221 agcgcaaagg caatgggcgc agccaggaac gactggttgt cagcgtagtc catgacgggg
     5281 ataattcccg taagtacaag ggcaacaatt atatagagag tggagcatat caccagtgac
     5341 actatgattc caataggcag gttacgttgt ggatttttag tttcctcggc cgtggttgaa
     5401 acagcgtcaa atcctatata tgaaaagaat actatggcag cacctgtcat caccccggtc
     5461 cagccgaaag gcatgaacgg tttccagttt tcgggtctga tgaaaaagag cccgagtata
     5521 atgaagaaca ggattactgc cagtttgatg aagacaatta tgctgttgac ctttgcgctt
     5581 tcctttatgc ctatcacgag cagggtggtg attatgccaa caattatcag tgcagggagg
     5641 ttaaacggcg gtgcgcacat ccatgcagga atgattatac ccaacccggc aagcaggttt
     5701 acgaggtagc ctgaccagcc tatagctacc gttattgagc acacggcata ttcaaggata
     5761 aggtcccagc cgattatcca tgccagaagc tctcccatgg ttgcgtaggc ataagtatat
     5821 gcgctgccgc tgacgggaat catggaggca aattcggcat agcacaatgc ggcaaaaaca
     5881 caggcaattg ccgacacgcc gaatgacagg gttatggccg gtccggcatg gagtgccgca
     5941 ccgacaccgg taagaacaaa aatgccggtg ccaattatgg ctccgatgcc cagaaggacc
     6001 aggtcaaatg atcccagtgc ccttttgagc ttatggtcac cctcttcggt ctgggcgatc
     6061 agctcctcaa ccgatttttt tgcaaagaaa ttgttggcca tatattttta ttgagttcag
     6121 acattcagtt tgctgtgctt cctgccgtag aggaaataga taaggaagcc gattactgtc
     6181 cataccccga ggcggaacca tgtagatccc ggaagggaaa acatcaggta aaagcatcct
     6241 gccgcacctg cagtacctac aaaatagacc acgggcgtac ggaacggacg gttcatatca
     6301 ggcctcctta ccctgaggat cattatactt atacagacca tggcaaatgc aaggagggtt
     6361 ccgattgata ccagttcacc tatgaggtct atcgggaaaa gtcccgaaat cactgcaatt
     6421 attgatccga ctataattgt tgttatgtgt ggtgttctgt attttttgtg tacccttccc
     6481 agaacaggag gcagaagccc gtcatttgac attgagtaaa ggatccttgg catgcctgta
     6541 atcagtacaa ggatcacaga gctcatcccg gcgatggctc cgatcttgat tattggcctg
     6601 agccagaaca gacccgggcc tgcagaatct attccaactg caatcgggtc aggaacattg
     6661 agatacttgt agttcaccat tccggtaaga accagagata cggcaatata aaggattgtc
     6721 acaataatca gtgatccgag aattcccttt gccatgtcac gctggggatt ctttgcctcc
     6781 tgggcaacag tagaaaccgc atcaaatccc acaaaggcaa ggaagataat cccggcagcc
     6841 ctgaaaatgc cgctccagcc aaactcgcca tgtacgcctg tattttcagg gatgaacgga
     6901 acataattat ccggatttat atatgacagc ccgaatccga tgaaaaggag aatcactgcg
     6961 atctttataa cgacaattat attattgaac ctgaccgatt cctttattcc gaagacgagg
     7021 accgttgtta tcagggctac tatgaagatg gaaggaagat tgatcatggc tcccgtggta
     7081 atccagccat catgataatc aagaggtgca ttgcataacc cgggggggat tactatacca
     7141 aagtctttca ggaagctgac tacatatcct gaccatccca ctgcaaccag tgaagctccg
     7201 aagatatatt cgagtaccag gttccagccg ataaaccatg ccaggaactc accgacggtt
     7261 gtgtatgcat aggaataagc acttcccgca atagggatca tggaggcaaa ctccgtataa
     7321 cagagtgcca cgaacatgca gacaatggca gaaatcagaa atgatatgct aagggccggg
     7381 cctgcatact gggccgctgc cgtaccggta attacgaaaa tgcctgtacc aataatgcat
     7441 ccaatcccga tgaaaatcag gtttgttgct gacaaagcgc gtttcaatcc atgctcagaa
     7501 tctgacgcgg tggccatcat ctgctgaagt gattttgttc tgaagagttt attcgccatt
     7561 cctgtgataa gaagtttcca tttcagtctt ggtttccctg gctggccacc ggccgccggt
     7621 aataaattgg agtaatcagc cctcaggtct cctcaaatat atattgaggc taaaagtata
     7681 gaaataatcc attgtctgct acggcaggcg acaatgttgt acagtagttt ttataaaaaa
     7741 gctgccacat gtgtgacagc tctggattct ttcattgcga tttaaggccc tgatcccggc
     7801 tggtgcccca ttggggtgcc gttatctttt gcaaacgcag gcctcagacc tgccgctggt
     7861 atctaaaacg acgtgatctt atccacgttc gtgttggcat acatgatctt cagtgcgctg
     7921 gcgcgccgta gcgcctgctt cacgtcagtt actatgccca tcttcgtatc ccggtcaatc
     7981 ttgagaacaa ccagcatctt ggctttgtca gcctcgttga gctgttcgcg ctcctgtgcg
     8041 ataaaatcct ggatatctgc cagttcctta aaactgtcat taagctggat tttcggctct
     8101 ttaccataga gggcctggaa tgactcgagg ggttcaccga tatagatgaa actggcgagc
     8161 gattttttct cgagcacctt tccttcggtg gcctcaggga tgcgaacggt gaccataaca
     8221 tcggtctcgc gcattgtggt gacagccatg aagaagaaga ggatcatgaa gacgatgtca
     8281 ggcaatgatg atgtgctgat tgccttgatg gttttcttcc ctgtctttac aaatcttgcc
     8341 atccttctaa tctcctatcc ttttgggttc agcttctgaa ataggtacct tgtaatagtc
     8401 gtcaacagca gacttgcggt cttcatccag tctatcatag ggtatcccga actcctgtct
     8461 ggcaacgtca tcccgaagct tggcccttgc tgcaataagt tcgttctgca ccgccaggta
     8521 aacgccgtat ttggtgccga tatcattctg caatgatata actgccttgg tgacagccac
     8581 attgccaaag aaggggagat aaacaacctc tctctcgggc atggaaggtt catcatttgg
     8641 attgagaatg aaattatggg caatctctct caactgatcg aactcggtaa gctgcccttc
     8701 cacgagtaaa aggttgtctt tattgacctt tacttcgagg atgtttctgt cattgatctt
     8761 attgacatcc tgcggaacgt ttggatcagg tattgcgggc agctgacgca tcagccccgt
     8821 gtcagaatcc atagtggtgg cggccaggaa gaaaataaga aggaggaacg caatatcagc
     8881 cattgagcct gcattaattt ccggtacttg tcttcttgcc atttggtcag aaatttactc
     8941 cgccgcaggg cggacagttc agttactttc taaaatactg tgaaatcaca gagaatataa
     9001 tggtcagaaa agctccggcc agaagaaaat aggcaacata taggcctata tcagtcatga
     9061 acagtgtgcc tggtacgtta tccttgcctt tgtaatgagg caggtcaagc acggtgttgt
     9121 cagccagcag tgcagatata ccgattagca cggcaaaaac tcccagagca atgagcagac
     9181 ccttcaggcc cttaggatta atgacgtagc ttcgcactgc gaagaagaga agggtaagga
     9241 cagtgaccgc gagcagaaca tatgcgtaaa cgatgaaaga gttggtcacc ttaggttcat
     9301 ggtaccttgt tccttcagtg cccggcacaa cgccgcctac ataaaacaag atggcgaaaa
     9361 tcactgtaat gcccatgagg acccacagga ggatcaatgt cagcctggtt attttttcag
     9421 acatatctgg ttatttttga ttgtatttgg ccagcatatc aatgagggtg atagatgaat
     9481 cctccatctg gtttgatatc ccgtcgattt ttgaaagcag gtaattgtag aatacctgga
     9541 gaataatagc cacaatgagg ccaaatactg tggtgataag ggcaacctta ataccaccgg
     9601 ctacgatggt tgctgataca gttcctgcct gttcgatggc gtcaaatgcc tgtatcatcc
     9661 cgataaccgt tccgaggaac ccgagcatgg gagcaatagc tataaagagc gagatccatg
     9721 agaggttttt ctcaagcaga cccatctgta cgccaccgta ggaaatgact gatttttcag
     9781 ccatctcaac gccttcatgt gccctgtcga agccctggta gaagatgctt gccacagggc
     9841 cgcgggtatt gcggcagatt tcctttgcag catcaatacc ctcttcattg agggctttgt
     9901 taacctttgc gagaagcttg tcagtgttgg tcgtggccag gttgaggtag ataattcttt
     9961 caattgcaat cgcaagaccg aagatgaggc agagaaggat aacacccatg aagcctgcgc
    10021 ctccctcaat gaacagtcgc ttgatctcct tgtggaggac tgtttctccc tgtgccggag
    10081 ttgtagcctg ggcgtcaagt tgccgggttg tggcagcctg agcctgctct gttgtctgtt
    10141 ggtttgttgc agtctggtcg gtctgtgcct gtgatgtctg tgcagtgccg ataagcagca
    10201 gagctgcaaa tgccataaat acaagaactc ttttcatcat gtcagattat taataggaat
    10261 atcctgacaa aaatataaaa aaacggtaac cagacaacgg agaatataat tttatataaa
    10321 ttaacgatgg gagatgttgc catacaatgc tcacgaagca ccctcaggaa agatcttgtc
    10381 ttccttgtaa aagaagggta ttactgaaga caggtgaccg tagaggcacc atgtgtcaca
    10441 aagcttttca tgtagaaaaa ctacagtaat ttacaccccc attacatacg ttcatcccag
    10501 atcttagggt cacggcttgt gtggaacagg gcttctactt taaccgtttt cgtttgctcg
    10561 tttacacggt aatgtaccag atacgggaat ttttcaatac acatgcaccg gatatcgtca
    10621 tattttacgg caaaatgcag ggcagtagtg gagagttttg ttgtttgttt cttgatattg
    10681 ttaagcagcc tgttgcccag accggcttgt ttttcattat accagtcaac tgcctgaaca
    10741 atgtcgttaa aaaaatcggg gttgtattct accgtgaatt tcattcgcgg gcagatattt
    10801 tacgttcaat gtcattccat gaaagatagc tgtcaggact attttcatat
//
LOCUS       DAOPBK010000087        10667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6035_length_10667_cov_5.441670, whole genome shotgun sequence.
ACCESSION   DAOPBK010000087 DAOPBK010000000
VERSION     DAOPBK010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6035_length_10667_cov_5.441670"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLA17_06445"
     CDS             <1..257
                     /locus_tag="PLA17_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPK85090.1"
                     /translation="IDNLLAALDESGRERLEARTEIIIVPGYRFRMVSSMLTNFHQPH
                     STLLLLVAAFTGHSWKDIYNHALAGGYRFLSYGDSMLLAP"
     gene            complement(449..1498)
                     /locus_tag="PLA17_06450"
     CDS             complement(449..1498)
                     /locus_tag="PLA17_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HPK85091.1"
                     /translation="MSNPELRRGFASDNNSGVHPAIMKELLRVNRGHVVGYGDDPFTE
                     TAKELIRKELGDETEVFFVFTGTGANVLGLAGVMKSWNSVITAHTAHIETDECGAPEK
                     FVGCKVLTVDTPDGKLHPEMLARHMHGFDFEHHSQPGVISISQATEMGTVYTSGEVKE
                     LADYAHAHGLLLHMDGARIANAAVTLGVPFREFTSGAGVDILSFGGTKNGMMYGEAVC
                     FLKPGLAEGFKYIRKQGMQLASKMRFIAAQYIAYLSGGLWKDCASHSNRMARELQRRL
                     KDISGIKITQKVESNGLFVIMPDRVAAQLSREYFFYPWNEQTSEYRLMTSWDTTSDDI
                     VQFADKLKSMMSEYA"
     gene            1588..2340
                     /gene="truA"
                     /locus_tag="PLA17_06455"
     CDS             1588..2340
                     /gene="truA"
                     /locus_tag="PLA17_06455"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPK85092.1"
                     /translation="MTRYFVFLSFRGTAYNGWQIQPGKHTVQGVLTKALSTVLSSQIT
                     VTGAGRTDTGVHASFFCAHFDAMRNDLDSDRQLLYNLNSILPDDIAINRIVKVKPDAN
                     ARFNAISRTYQYTVTRVKDPFCADTVWLLWWQLDIDRMNEAAAILLKHSDFTSFCRLH
                     GSNKTNICRVTHARWSEEQERLIFTITADRFLRNMVRAVVGTLIPVGRGKLTLNEFEA
                     ILRGKDRGLAGQSAPAHGLSLTAIEYPGEIFD"
     gene            complement(2609..3361)
                     /locus_tag="PLA17_06460"
     CDS             complement(2609..3361)
                     /locus_tag="PLA17_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPK85093.1"
                     /translation="MKLYGIIGFPLGHSFSQKHFSEKFEREHLSDYTYRKFELPEIGM
                     LPELLIREPDICGFNVTIPWKVKVIDWLDDIDQLAAEIGAVNTVKVTRRGGKAWLKGF
                     NTDAPAFRDSLLNNLDTLPDTALVLGTGGAAKSVIHTLAELNITAIPVSRKPVRGGYT
                     YDDLPGYLISEAGIIVNATPVGMAPHTEAMPPINYNCLRERQLLFDLTYNPPVTAFLA
                     MGEERGCITVNGEEMFLIQAGLSWEIWNDEER"
     gene            complement(3358..4137)
                     /locus_tag="PLA17_06465"
     CDS             complement(3358..4137)
                     /locus_tag="PLA17_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HPK85094.1"
                     /translation="MKQARQLLTHIPERPVKPRKEGLTMVMDKGLSLREAEDFAETGS
                     EYVDFVKLGFGTSVITPNVDKKIKAYHKAGMIVYFGGTLFEAFIIRGLFDKYVDLLKS
                     AGVRMVEISDGSYEFDHSEKLQYIRKLSKDFTVISEVGSKKKDIIYTPDQWVELMKSE
                     LDAGSVKVIAEARESGTIGIYNDDGSVNKELIETISREIGFDNVLWEAPLKQQQAWFI
                     ANFGANVNLGNIAPNEIIALESLRLGLRSDTFFSFLPDNMK"
     gene            complement(4141..5547)
                     /locus_tag="PLA17_06470"
     CDS             complement(4141..5547)
                     /locus_tag="PLA17_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK85095.1"
                     /translation="MEEERSGFVHDSELDKVILRFERMRKNKETCYFDVNEFETIIDY
                     YLEGNNPVNAFEATSLACELHPNSVPILIRKARVLLEKGRATEALTIAKRLENIEPGN
                     CDIYLCKATALAMLGDINGARKVFDLALETDSDDIENILYSITLVLQNLNFHEEMIPY
                     LHRLAELEPDIPAHLYDLAYAYDKKGDHQTSIKYYNLYLDEDPYSDSAWYNLGIIYNK
                     TGDYGKSLEVYDYALALNPENTFALFNKANILSNLGKYNEAMDVYRQFVEEEPDSLEG
                     LTYLAECYDKTGDEAMARKYYAEAIDITPDFPDPWYGLGLLSLSRNRPAESIMPLKKA
                     IELDPENPDYWFSLGRAHIMNGNAMEAIRCFIETLIIDGYYDEAWIETGMVVMMTGAY
                     RGAIRILKKAAKISGDLPGIYFLLASAYLHTGNIKEATRNLLRGIRLDNELLEEYSVL
                     LPEEKLTNAMRRLFRKTN"
     gene            5892..6851
                     /locus_tag="PLA17_06475"
     CDS             5892..6851
                     /locus_tag="PLA17_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85096.1"
                     /translation="MAIILFSTGCREEPLTINYKLGIFPDSVMALEGLNTVYDDYNMD
                     IEAGRIDARIPVVFSSNRQSAGGEFDLTHGLIWYTFGQTTGSFQLGSEIAADVFLDRL
                     TAAFNTGGNEFGPLRFFNGRNGLEYMAAATESDENGLEIVYTSYIPAFTNLPAVEPPV
                     GATLFNSPSDDAYLSLGTTLDTAWFCSDRGGDFDIYMLTRPAGLSLDEWFMSPAMTPT
                     PVDSINSTGNDKCPFVRGRYMVFASDMPGGMGGFDLYYSVFRNGKWSSPVNMGPGINS
                     AANEYRPVLGKDLKFENYFLIFSSDRPGGKGGYDLYFTGISMH"
     gene            complement(6824..7711)
                     /locus_tag="PLA17_06480"
     CDS             complement(6824..7711)
                     /locus_tag="PLA17_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HPK85097.1"
                     /translation="MNILDGKKTAAAIRGEIAETVKEIKNSGHRVPHLGAILVGNNSS
                     SETYVSHKVTDCQEVGFRSTLIRMPATVSEEELLGKVRELNGDPELDGLIVQLPMPVH
                     ISEDKVIETISPDKDVDGFSPVNMGRMVAGLPSFVPATPAGIMELLKRYNIPLKGAEC
                     VVAGRSNIVGRPVSILLSQKGVDATVTVVHSRTRNIAEIIRRADIIIAAIGQPGFITA
                     DMVKEGAVVIDVGITSVEAPATKTGWKLKGDVDFENVSPRCSWITPVPGGVGPMTRVA
                     LLLNTLQSYRAQCMLMPVK"
     gene            complement(7755..9077)
                     /gene="ffh"
                     /locus_tag="PLA17_06485"
     CDS             complement(7755..9077)
                     /gene="ffh"
                     /locus_tag="PLA17_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPK85098.1"
                     /translation="MFESLSERLERSFKILKGEGVISEINVSETLKDVRRALLDADVN
                     FKIAKQFTDTVKQKAMGQNVLKSVKPSQMMIKIVHDELIELMGGEKSDINIRANPSII
                     LIAGLQGSGKTTFSAKLARFLKSKRGKHPLLVAGDVYRPAAIEQLKVLGSQIEVPVYT
                     EEGSRNPVEIAKNAVREAKKNGNDLVIIDTAGRLAVDEEMMNEIAAVKNAVNPHETLF
                     VVDSMTGQDAVNTAFEFNERIDYDGVVLTKLDGDTRGGAALSIRSVVNKPVKFVSTGE
                     KMDAIDIFYPERMADRILGMGDIVSLVEKAQEQYDEAEARKLQKKIAKDQFDFNDFIS
                     QIQQIKKMGNMKELMGMIPGVSKAVKNLDIDDNAFKGIEAIISSMTPAERSNPMLLNG
                     SRRKRIAEGSGTTVMEVNRLLKQFDETRKMMKMVAKGGNLSRMAVKKR"
     gene            complement(9099..>10667)
                     /gene="argS"
                     /locus_tag="PLA17_06490"
     CDS             complement(9099..>10667)
                     /gene="argS"
                     /locus_tag="PLA17_06490"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPK85099.1"
                     /translation="GYNVIRGFLNLTISPAEWGRRLDEMHADTGYGFSQPGQDAETVV
                     IEFSSPNTNKPLHLGHIRNNLLGFSLSRITGAAGNRVIRTNIVNDRGIHICKSMLAWQ
                     KYAKGRTPLSEGIKGDHFVGDYYVLFEKKLKEQAAELVAAGTTPEEARENAPLMQEAR
                     RLLLKWEAGDPETVQLWQMMNSWVYEGFDVTYRALGVEFDKIYYESDTYKVGREIVLR
                     SLEQGDLIRKEDGSVWIDLTEERLDQKLLLRSDGTAVYMTQDLGTAVIRYDDYHFDRH
                     IYVVGNEQNYHFQVLTLALQKMGFEWARGLHHFSYGMVELPEGKMKSREGTVVDADDL
                     IEEMIATAAVMSQELGKLEGYSQDEKDNICRQIGLGALKYFILKVDPKKNMTFNPQES
                     IDFNGNTGPFIQYTCARIRSVMKKAETTGLADSSDAVEGVIPNPKEICVIKLLNDFRQ
                     IVPEAARELSPALIANYCYDLAREFNQFYHDYSILGEEDKNRRNFRLRLSELTGRVLE
                     KGLYLLGIEAPERM"
BASE COUNT         2391 a         2812 c         2558 g         2906 t
ORIGIN      
        1 caattgacaa tctgctggct gccctggatg agagcggcag ggagagactg gaggcacgta
       61 ctgaaataat cattgttcca ggatacaggt tcaggatggt cagcagtatg ttaaccaact
      121 tccatcagcc ccacagcact ctcctgctgc tggttgcagc tttcaccggc cattcatgga
      181 aggatatcta caatcatgca cttgccgggg gataccggtt ccttagctac ggcgacagca
      241 tgctgcttgc tccgtgaagc tgaatcacaa ggcagggaaa gttggctcag attattgaaa
      301 agccggctcc gaaccctggt tatttcaggc tgcttttgtt atctctttcc ctgtagattt
      361 gatacagaca gggttgagtc atttcacgcc tggagattta taaggaaggc tgataccctg
      421 tagccgaaat agagaaaggg ttgagagact atgcatattc tgacatcata ctcttcagtt
      481 tatctgcaaa ttgcacaatg tcatctgaag ttgtatccca gctcgtcatc aaccggtatt
      541 ctgatgtctg ttcattccac ggatagaaaa agtactctct gctcagctgt gcagccaccc
      601 tgtcgggcat gatgacaaag aggccatttg actccacctt ctgcgtgatc tttattccgc
      661 tgatatcttt aagtcttctc tgcagttccc gtgccatcct gtttgagtgt gaggcacaat
      721 ccttccataa tccgcctgaa agataggcta tatactgtgc tgctatgaaa cgcatctttg
      781 aagccagctg catcccctgc ttcctgatat atttgaatcc ttcggcgagt cccggtttaa
      841 ggaaacagac agcttcgccg tacatcattc cgttcttggt tccaccgaat gagaggatat
      901 caacgccggc gccgctggtg aattccctga acggaacgcc cagtgttact gcagcgtttg
      961 ctattcgggc gccatccatg tgcagaagca gaccgtgcgc atgcgcgtag tctgccagtt
     1021 ccttcacctc cccggatgta tagacagtac ccatttccgt ggcctgtgag atggagatca
     1081 cccctggctg gctatgatgt tcaaaatcaa atccgtgcat atgtctggca agcatctccg
     1141 ggtgcagctt cccgtcaggt gtgtcaacag taagcacctt gcatccgaca aatttttcag
     1201 gagcgccgca ctcgtcagtc tcgatatggg ctgtatgcgc agtgatgact gagttccacg
     1261 acttcatcac cccggccagt cccagtacat tggcgccggt gcctgtaaag acaaaaaata
     1321 cctccgtttc atctcccagc tctttcctta tcagctcttt ggccgtttca gtgaacggat
     1381 catcgccgta accaaccaca tgccctctgt tgacccgcag cagttccttc ataattgcgg
     1441 gatggacgcc tgagttatta tcgcttgcaa agcctctcct gagttccgga ttgctcatga
     1501 atttattgtt taattggttg ccgcaattta gtcaaaagta acctgccggc caatgttctt
     1561 tggctatctt tacatttttg ccaggatatg acacgatact tcgtttttct ctccttcagg
     1621 ggcacagcgt acaacggctg gcagattcag cccggcaagc acaccgtaca gggtgttctc
     1681 acgaaagccc tcagtacggt tctctcctcg cagataacgg tcacgggcgc cggccggact
     1741 gataccgggg tgcatgcctc gtttttctgt gcccattttg atgccatgcg caacgacctc
     1801 gacagcgaca ggcaactgct ttacaatctc aacagcatac ttcctgatga cattgcgata
     1861 aaccgtatcg tgaaggtaaa acctgatgcc aatgcaaggt ttaatgctat ttcccggaca
     1921 tatcagtata ccgtgaccag ggtgaaggat ccgttctgcg cagacaccgt ttggctgctc
     1981 tggtggcagc ttgacattga caggatgaat gaggcagcag cgatacttct gaagcacagc
     2041 gactttacca gtttctgccg gcttcacggc agtaacaaaa ccaacatttg cagggtgacg
     2101 catgcgcgat ggagtgaaga gcaggagagg ctcatcttca ccattaccgc tgacaggttc
     2161 ctgaggaaca tggtaagggc agtggttggc actctaatcc cggttggcag aggtaagctg
     2221 accctgaacg agttcgaagc gattctcagg gggaaagacc ggggccttgc aggacaatct
     2281 gcacctgcgc acgggctttc gctgacggca attgaatatc ccggcgaaat atttgattga
     2341 ttttcaggtg tcagaagcta ttgaccggca gagtgccggc aagcaggaat aatgacatac
     2401 agccggcttc aggaaaagtg gcagagtgcc agagcagtag gaagagtata agagtgtcgg
     2461 cagctgttga ccggcagagt gccggcagca ggaaaaacgg cagagcaccg gcagagtgcc
     2521 ggcaagcagg aatagtggca gactgcaggc atcaggaaga gtaacaggct aatggcagca
     2581 ggaagaactt caggaatccg cctgctcttt atcgctcctc atcattccat atctcccagg
     2641 acagtccggc ctgtatcagg aacatctcct caccgttgac ggtaatgcat ccacgctcct
     2701 ctcccattgc caggaatgcc gttaccggag gattgtatgt aaggtcgaac agcagttgtc
     2761 tctcccgcag gcagttgtag tttatgggag gcatcgcttc agtgtggggt gccataccca
     2821 ccggtgtggc attgactatt atccctgcct cgctgatcag gtatcccggc aggtcatcgt
     2881 atgtatatcc accgcgaacc ggctttcgcg ataccggtat agcagtgata ttcagctcag
     2941 caagtgtatg gatcacgctt ttcgcggctc cgcctgtacc gaggaccagg gcagtgtcag
     3001 gcagggtatc caggttattg agaagtgaat ccctgaaggc aggtgcatct gtgttgaagc
     3061 ctttcagcca ggctttcccg ccgcggcggg tgacctttac ggtattgact gcgccgattt
     3121 cggcagctag ctgatcaata tcatccagcc aatcaatcac cttcaccttc caggggatgg
     3181 tcacattgaa tccgcagatg tcaggctcac ggatcagcaa ttcagggagc atgcctattt
     3241 ccggcagttc aaacttacgg taagtgtagt cggaaaggtg ttccctttca aatttttccg
     3301 agaaatgctt ttgagagaat gaatgcccga gcggaaagcc aattattccg tagagcttca
     3361 tttcatgttg tcaggaagga agctgaagaa ggtgtcactt cgcagtccaa gcctgagtga
     3421 ttcaagtgca attatctcgt tcggggctat gtttccgagg ttgacattgg ctccgaagtt
     3481 ggcaatgaac catgcctgct gctgtttcag cggtgcttcc cacaggacgt tgtcaaatcc
     3541 tatctcccgt gagatggttt cgataagctc cttattcaca gagccgtcat cattgtatat
     3601 gcctattgtg cctgattcgc gcgcctcagc gataaccttg accgagccgg catcaagttc
     3661 ggacttcatc aactccaccc attggtcggg ggtatagata atgtccttct tttttgagcc
     3721 aacctcggag atcactgtga aatcctttga gagcttcctg atatactgaa gtttttcact
     3781 atgatcgaac tcatatgagc catctgagat ttcaaccatt ctcacgcctg cactcttaag
     3841 cagatcaaca tatttgtcaa acaaacccct gatgatgaat gcctcgaaca gggttccccc
     3901 aaagtagaca atcattcctg ccttatggta ggcttttatc tttttgtcaa cattcggggt
     3961 tattaccgat gtaccgaatc caagtttcac aaaatcaaca tactcgctgc ctgtctcggc
     4021 aaagtcttca gcctccctga ggctcagtcc cttatccatt accatcgtga gcccctcttt
     4081 tctcggcttc accggtcttt ccggaatgtg tgtcagaagt tgtctcgctt gtttcatatc
     4141 ttagttggtt ttcctgaaca gtcgtctcat tgcgttggtc agcttttcct ccggcagaag
     4201 cacggagtac tcttcaagca gttcattgtc aagccttata cctctcagca ggttgcgggt
     4261 ggcttctttt atattgccag tgtgaaggta agccgaggcc agcaggaagt atattccggg
     4321 caggtctccg ctgattttag cggctttttt caggatccgg attgcgcccc tgtaggctcc
     4381 ggtcatcatc accaccatgc ctgtctcaat ccatgcctcg tcatagtagc cgtcaattat
     4441 cagggtctcg ataaaacacc ttatggcttc catggcatta ccgttcatga tgtgggccct
     4501 gccaagagag aaccagtagt cagggttctc aggatcaagc tcgatggctt tcttcaatgg
     4561 catgatgctt tcggcaggcc tgttgcgcga gagtgacagc agccccagtc cgtaccacgg
     4621 atcaggaaaa tcaggagtga tatcaatggc ctcggcgtag tacttgcgtg ccatagcctc
     4681 atcgcctgtc ttgtcgtaac actcggcaag gtatgtcaga ccctccaggc tgtcaggctc
     4741 ctcctcaacg aactggcggt agacatccat ggcctcgtta tatttcccga gattgctgag
     4801 gatattggct ttgttgaaga gagcaaacgt gttttcggga ttcagggcaa gggcataatc
     4861 atatacttcc agtgactttc cataatcacc tgttttattg tatattatgc caagattata
     4921 ccatgcactg tctgagtacg gatcctcatc cagatagagg ttatagtatt ttatgcttgt
     4981 ctggtggtca cccttcttgt cataggcata tgcaaggtca tacagatgag cagggatatc
     5041 cggctcaagt tcggccagcc tgtggaggta gggaatcatt tcctcatgaa aattgaggtt
     5101 ctgcagcact aatgttatgc tgtagaggat attctcgatg tcatccgaat cggtttccag
     5161 ggcaaggtcg aacacctttc gtgccccgtt gatgtcaccc agcattgcca gcgccgtcgc
     5221 cttgcaaagg tagatatcac agtttcctgg ctcaatgttt tcaagccgtt tggctatggt
     5281 aagagcctcg gtggcccttc ccttttcaag cagtacgcgg gctttcctta tgagaatggg
     5341 aactgagttg ggatgaagtt cgcatgccag tgaggtagcc tcaaaggcat tgaccgggtt
     5401 gttaccttcc aggtaatagt ctataatagt ctcaaactca ttgacatcga aatagcatgt
     5461 ttccttattc ttccgcattc tctcaaaccg gagaataacc ttatccaact cactgtcatg
     5521 cacaaaaccg gatctttctt cttccatctc cccgagaaaa tattctgcaa atgtaatata
     5581 taattgtttc agtcagccct gaccgggcag caattatcaa caggcgtcta tttttataaa
     5641 catgataaac agctgtttgt aaaaataaaa tcgatctgac gcaacctttc cgtgtaagat
     5701 tccatcttac aattgagtat tcggaggatt accctcaaac caatatatta ccctagaatt
     5761 aacctgaatt ctaccttctg aaaaccgggc tacaagtccg gtttttttca tctggctcat
     5821 attaaacatc ttacttgccc gttatagaat aaacgactgt catgaccagc aggttattat
     5881 tcttttgcag tttggcaatt atccttttct ccacgggctg tcgcgaggaa ccgttgacaa
     5941 tcaactataa gctgggtatt ttccctgatt cggtcatggc ccttgagggg ctgaatacgg
     6001 tgtatgacga ttacaatatg gatattgaag cgggaaggat tgatgccagg attccggtag
     6061 tcttctcctc caacagacaa agtgcgggag gtgagtttga cctgacccat gggcttatct
     6121 ggtacacctt cggacagaca accggttcat tccagcttgg atctgagata gcagcagatg
     6181 tttttctcga caggctcacc gcagctttca acaccggtgg caacgagttc ggtccgttaa
     6241 ggttctttaa tggccgcaac ggcctcgagt atatggcagc tgctacggag agtgatgaaa
     6301 acggactgga gatcgtatat acaagttaca taccggcttt caccaacctg ccggcagttg
     6361 agccccctgt cggggcgacg ctcttcaaca gcccgtctga tgacgcctat ctatccctgg
     6421 gaaccactct tgacacggca tggttctgtt cagacagggg cggtgatttt gatatctaca
     6481 tgcttaccag gcctgccgga ctgagcctcg atgaatggtt catgtcaccg gcgatgactc
     6541 ccacgccggt tgacagcatt aacagcacag gaaacgacaa atgccccttt gtcaggggaa
     6601 gatatatggt atttgcctct gatatgcctg gcggaatggg cgggtttgac ctctactact
     6661 ccgtcttccg caacgggaag tggagctctc cggtcaatat gggtcccgga atcaactccg
     6721 cagcaaacga gtacaggccg gtgcttggaa aagaccttaa gtttgaaaac tatttcctga
     6781 tattctcgtc tgacaggccg ggcggcaagg gagggtacga tctctatttc acaggcataa
     6841 gcatgcactg agccctgtag gactgaagag tgttcagcag cagggcaacc cgggtcatgg
     6901 gccccacacc tccgggaacc ggggttatcc aggagcaccg gggtgagacg ttctcaaaat
     6961 caacatctcc cttcagcttc catcctgttt tggtagcagg tgcttctaca cttgttattc
     7021 caacatcaat taccactgct ccctccttta ccatgtcagc ggtaatgaat ccaggctgcc
     7081 cgatggcagc gattatgata tctgctcttc taataatctc cgctatgttt cttgtgcggc
     7141 tgtgaaccac ggtgacggtt gcatcgacac ctttctgtga caggagtatg ctaaccggac
     7201 ggcccacgat gttgctgcgg cctgccacaa cgcattcagc gcccttcagg ggaatgttgt
     7261 accgtttcag caactccatg atgccggcag gtgttgccgg gacaaaggag gggagtccgg
     7321 caaccatacg gcccatattc accggactga agccgtcaac atccttatcc gggctgatgg
     7381 tctcaattac cttgtcttca gagatatgga caggcatggg cagttgcacg atcaatccgt
     7441 caagttcagg gtcaccgttc agctccctga ccttgccgag caactcctcc tcgctcacgg
     7501 ttgcaggcat tctgatgagt gttgacctga agcccacctc ctggcagtca gtcaccttgt
     7561 gggaaacgta ggtttcgctg ctgctgttgt tgcccaccag gatggctccc agatggggaa
     7621 cgcgatggcc tgaattcttt atctctttca ccgtctcagc gatctcacct ctgattgctg
     7681 ctgccgtttt tttcccgtcg aggatattca taattgcgat tcaaatgtta acaatgctcc
     7741 atggtctctc ctgctcaccg ttttttcacc gccatcctgg acaggttacc tcctttggca
     7801 accatcttca tcatcttgcg tgtctcatca aactgtttca ggagccggtt cacctccatg
     7861 acagttgtgc cactcccttc ggcgattctc ttgcggcgac tgccgttgag cagcatcggg
     7921 ttcgatctct cggccggagt catgcttgag atgatggctt ctattccctt gaaggcatta
     7981 tcatcaatat caaggttttt gacagccttg ctcaccccgg ggatcattcc catcagctcc
     8041 ttcatgttcc ccatcttctt gatctgttgt atctgagaaa tgaagtcatt gaagtcgaac
     8101 tggtcctttg caatcttttt ttgcagcttg cgtgcctcag cctcgtcgta ttgctcctga
     8161 gccttctcca ccaggctgac gatgtcgccc atgcccagaa tcctgtcagc catcctttca
     8221 ggatagaaga tgtcaattgc atccatcttc tcgcctgtgc tgacgaactt gacaggcttg
     8281 ttcaccactg atcttattga aagggccgca ccacccctgg tatcaccgtc gagctttgtg
     8341 agaaccaccc cgtcgtagtc tattctctcg ttgaattcga atgccgtgtt gactgcatcc
     8401 tggcctgtca tcgagtcaac cacgaagaga gtctcatgag gattgacggc attcttcacg
     8461 gccgctatct cgttcatcat ctcctcatca actgcaaggc gaccggctgt gtctatgatt
     8521 accaggtcgt tgccattctt ctttgcttcc ctgacggcat ttttggctat ctccaccgga
     8581 ttgcggctgc cctcttcggt atataccgga acctctatct gtgatccgag caccttcagc
     8641 tgctcaattg ctgcgggcct gtaaacgtcg ccggcaacca gcagcgggtg cttgcccctc
     8701 ttgctcttca gaaacctggc cagcttggcg ctgaaggtgg tcttaccgct tccctgaagt
     8761 ccggcgatca agattatcga aggatttgcc ctgatgttga tgtcgctctt ctcccctccc
     8821 atcagctcaa tcagctcatc atgcacgatc ttgatcatca tctgtgacgg cttgactgac
     8881 ttaagcacat tctgacccat cgccttctgc ttgacggtat cagtaaactg tttggctatc
     8941 ttgaagttaa cgtcagcatc aagaagcgct ctgcgcacat ccttgagagt ttcggaaaca
     9001 ttgatttcag agatcacccc ttctcccttc agaatcttga aggagcgttc cagtctttca
     9061 ctgaggcttt caaacataat tatctgataa tatatatttt acattctttc aggagcctcg
     9121 atacccagca ggtagagccc tttctccagc acccttccgg tcagttccga cagccggagg
     9181 cggaagttgc gtctgttttt gtcctcttca ccaaggatag agtaatcatg ataaaactgg
     9241 ttgaattccc tggccaggtc ataacagtag ttggcaatca gggcaggact cagttcccgt
     9301 gctgcttcag gcacaatctg ccggaaatca ttcagcagtt ttatcacaca gatctccttg
     9361 ggattgggaa ttactccttc aaccgcatcc gaactgtctg ccagcccggt tgtctcagct
     9421 tttttcatta ctgaccttat cctggcgcat gtatattgta tgaaggggcc tgtattgcca
     9481 ttaaagtcga ttgactcctg cgggttgaag gtcatgtttt tcttcgggtc aaccttcagg
     9541 ataaagtact tcagggctcc caatcctatc tgcctgcaga tattatcctt ttcatcctgg
     9601 ctgtaacctt ccagcttacc aagttcctgt gacatcacgg cggctgtggc aatcatctcc
     9661 tcgatgaggt catcagcgtc aaccacggta ccttcgcgcg atttcatttt tccttcaggg
     9721 agctctacca ttccgtagga gaagtggtgc agtccgcgtg cccattcgaa tcccattttc
     9781 tgcagcgcca gtgtcagcac ctggaagtgg tagttctgtt cattgcccac cacgtagata
     9841 tggcggtcaa agtggtaatc atcgtatctt atgactgcgg tgcccaggtc ctgtgtcata
     9901 tagaccgcgg tgccgtcaga ccggagcagg agcttctggt cgagccgctc ttcggtgagg
     9961 tcgatccaga ctgaaccgtc ttccttgcgg atcaggtctc cctgctcaag tgagcgaagc
    10021 actatttccc ttccgacttt gtaagtatcc gactcatagt atattttgtc aaactccacg
    10081 ccaagcgccc tgtatgttac gtcgaagcct tcgtataccc aggagttcat catctgccat
    10141 agctgcaccg tttccgggtc gcctgcctcc catttcagga gaaggcgtcg ggcttcctgc
    10201 atcagcggtg cattttcgcg tgcttcttcg ggggtagttc ctgctgccac aagctctgct
    10261 gcctgctctt tcagtttttt ttcaaacaga acatagtagt cacccacaaa atggtcacct
    10321 ttaatccctt cggagagggg agtgcgtccc ttggcgtact tttgccaggc gagcattgat
    10381 ttgcagatat ggataccgcg gtcattgacg atattggttc ttatgaccct gtttcctgcc
    10441 gcacctgtta ttcttgagag ggaaaaaccg agcaggttgt tgcgtatatg gcccagatgc
    10501 agcggcttgt tggtattcgg cgatgagaat tcgatgacga cggtttcggc atcctgtccg
    10561 ggctgtgaaa aaccatagcc ggtgtcagcg tgcatctcat caagtcgtct gccccattct
    10621 gccggtgata tggtcaggtt caggaatccc ctgatcacat tgtagcc
//
LOCUS       DAOPBK010000088        10640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6053_length_10640_cov_4.733774, whole genome shotgun sequence.
ACCESSION   DAOPBK010000088 DAOPBK010000000
VERSION     DAOPBK010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6053_length_10640_cov_4.733774"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..2726
                     /locus_tag="PLA17_06495"
     CDS             96..2726
                     /locus_tag="PLA17_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPK85100.1"
                     /translation="MKRATALLFAALLLVSVSAQQYPFLDTGLSFEKRVDDLVSRMTT
                     EEKASQLLYTAPAIPRLGIPEYNWWNEALHGVARAGHATVFPQSITFANSWNEELMLD
                     VANAISDEARAKHHEFVRRGDRGIYHGLTFWSPNINLFRDPRWGRGQETYGEDPYLTG
                     RLGLRFVQGMQGSDPKYFKTIATAKHYAVHSGPEPVRHEFNAVISERDLRESYLPAFR
                     TLVKEGKVYSIMGAYNQFRDHPACANDELYGILRNEWGFDGYIVSDCWAVSDFYNFQG
                     YAAGPAEAAALALKAGTDLECGVDYRHLTEALKRGLITEEDIDRSVRRVMLARFRLGM
                     FDPDSIVDYAQIPYQANCSDYNRSLARKAARESIVLLKNSNGTLPLSRDIRTVAVIGP
                     NAASHEVLVGNYNGIPKNPVSLLEGIEAKLRPETEILYAGGSDLAPGMHNLEPIPSRY
                     LRTPDGAQGVLGAYYTSREMKGEPLFTRTDDMIDFYWDNDSPHPLMPVNDFGIHWVAD
                     LVPPVTGTYYLGTWGSSRYTIILEGDTLYSFYSDHHANIRGVPVDFRAGEKYRVEVLY
                     RNQGGDADMKLLWSIPKDDMKARAVEAASRADAVIVALGLSPRLEGEEQRLKLEGFEG
                     GDRTTLGLPAIQEELLAALLATGKPVVVVLMNGSPVASLQAHEKAAAMLLAGYPGEEG
                     GNAIADVLFGDYNPAGRLPVTYYRSVDQLPPFEDYDMDNRTYRYFRGTPLYPFGYGLS
                     YTTFAYSDLVVPAKAVAGTEVKVSVTVTNTGEMTGDEVVQLYLTDEKATTPRPLRQLE
                     GFDRLTLKPGESRRVEFTLKPEQFSMLNNRDTRVIEPGWFTVSVGGGQPGAMVQGAED
                     HVQVLTARLRLTGGEVVVKD"
     gene            2763..3068
                     /locus_tag="PLA17_06500"
     CDS             2763..3068
                     /locus_tag="PLA17_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPK85101.1"
                     /translation="MNPRIIEYREAIMIAFIGLGVNILSAFLLHHDHEESDYNIRAAY
                     LHVVADAITSVAAIVGLLTAMIWGLPYVDAIAALISSAVIIRWSLRLLRDSGRELIA"
     gene            3515..4888
                     /locus_tag="PLA17_06505"
     CDS             3515..4888
                     /locus_tag="PLA17_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HPK85102.1"
                     /translation="MMLPPEFLERIERQAYIDASGLAEALGGPSAASVRVNRFKWPHR
                     VSGYDRVEWEPDGFYLPGRPLYTSDPLFHAGVYYPQESSGMFTGEMFRQVTAGQEGLR
                     VLDLCGAPGGKSTHLASILGDDGLLVANEVIRSRSAILAENITKWGTGNVIVTNADPS
                     RFAQLPAFFDVVVADAPCSGEGMFRSPVAVREWSLQNANLCCERQRRIIMEVWPALKP
                     GGILIYSTCTFNPAENEENVEWIRKGTGAEPVMINLPPGSPVVPVRYGGTEGYGFYPG
                     RVRGEGFFIAAIRKPVTPAQDERAPDGRKTAVKPSPEAFEKTISLAAFDRERVVMHEG
                     RIIALAADAGLFGFIEARLPVIKAGTMIGELKKNELIPAHDLAMSARQRQEAWTRHSL
                     SREEALAFLRLEALNGSAMPEVRVLLTYRDVPLGFVKNLGNRVNNSYPRLWRIRMERE
                     TVMTEIL"
     gene            5111..6781
                     /locus_tag="PLA17_06510"
     CDS             5111..6781
                     /locus_tag="PLA17_06510"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPK85103.1"
                     /translation="MKTDIEIAQSASIRPVAEVAEQLGLKEDDLELYGKYKAKLPLTL
                     IDKKKAARAKLILVTAITPTPSGEGKTTTSIGLAQGLNRLGKRATVVLREPSLGPVFG
                     IKGGAAGGGYSQVIPMEDINLHFTGDLSAVEKAHNLLSAMIDNNIQSKEGNLGIDPRT
                     IAWKRVMDMNDRALRDVVIGLGGTAQGVPRETGFDITAASEVMAILCLATSISDLKAR
                     LGNIFIGYTYAGKPVFARDISAQGAMAALLKDALKPNLVQTLEGTPAIIHGGPFANIA
                     QGTNSILATRMGLSLSDYVITEAGFGSDLGAEKFFNIKSQAGKLRTNAVVIVATIRAL
                     KYHGGVRVPELVNENLEALKRGCENLDKHVENMHYYGFRPVVAINRFSSDTDAEIELL
                     KEYCEEHNVTAAVNTSWADGGMGAIELANAVIKASEACTDCFTPLYDHAWPMEKKIET
                     IATLMYGARNVEYTLEAKEQLKKISGLGLDKLAVCIAKTQKSLSDDQTRRNRPRDFTV
                     SIRKVELSSGAGFIVPIAGSIMRMPGLPAVPSAEKIDIDDNGLISGLF"
     gene            6862..7362
                     /locus_tag="PLA17_06515"
     CDS             6862..7362
                     /locus_tag="PLA17_06515"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPK85104.1"
                     /translation="MPVDGVTVISRTISLLEPLFSEIIVAGWPPDGPLPGDVILVHDR
                     YSGVGPLAGIEAALRVAGSPLLFVFGGDMPWLSEELIRAQVNEYIANPSDILVARSGG
                     LAEPLHSLYSRKIHTALVSHIEGGGSNTIIDFCKLAETRYYDIPLTLETRKALTNINR
                     PEDLLI"
     gene            7407..10637
                     /locus_tag="PLA17_06520"
     CDS             7407..10637
                     /locus_tag="PLA17_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK85105.1"
                     /translation="MRNSLLLMTLAALLLSSCQGYHDYSKTEWTEKDLPEWEDLTVNT
                     INTVTPHASMVSHPDNASALKAKWRESVNVLSLDGKWKFNYSPTPAERPYWFFREDFD
                     TRHWGEIEVPSTWEREGHGTAYYVNAGYTFPVNPPFINHSDNPVGSYKRSFTIPSGWK
                     DKEVFLNFDGVSSAFNVWVNGEKVGYSEDSKTTAEFNITPFLRKKKNDIAVEVYRYCD
                     GSYLECQDFFRLSGIKRSVWLHARPKTYIRDYFAVPTLVNEYTDGLLNLTVELAGNGG
                     RRPAAMRLEAGLYDGKTKILGEARDVQAFDSLAVVKFSAEVPKVNPWSAESPSLYTLV
                     ITLADNRGRVVESISSKIGFRTSEVKGGRFLVNGKAIYLKGTNMHEHDPVRGHTIDEA
                     LMLKDIKMMKMNNINAVRTSHYPQPERFYELCDEYGLYVIDEANIESHGIGYHPDVTL
                     AHKPEWLGQHMLRTVRMVERDKNHPSIIIWSLGNEAGDGQNFVETYKWIKGRDESRPV
                     QYERAEKSTHTTERHTDIWCPMYPSIERVESYAQSEGQPGFDRPYIMCEYAHSMGNST
                     GNLQDYWDVIEKYGILQGGFIWDWVDQGLLETTEEGEDYYAYGGDYGEPGSPSDGNFC
                     CNGLVGPDRTPHPALNEVWKVYQYVGFKPADLARGLVTIYNKYDFTNLSYFTIHWEVL
                     ANGALFRSGNLKQISLEPGDSLTVEIPWGEIVPASDTEYHLNLHVSRSDEWGIVPQDH
                     MYASEQMLLPFHAVTETGTASGAAGAPDGETLEIIDTRTEGSDLTFAGEGFEVVFDME
                     AGVMKSYRVAGQELIMSGPRPDFWRAPTDNDYGNGMEKRNASWREAGSRAVLKSARIS
                     HFAMGSAGVEFTFDIPGEGGMKIAGLVTGYRVHAGGSVDVSFNFTKTDMSLEEIPRVG
                     MQMILPGSFVDLSWFGRGPHENYVDRKTSAFVGVYESSVADQYVPYVRPQENGYKTDV
                     RWLNVTDGSGKGIRFEGEPLFSFAAMNYLHEDFESQGRLAGYRPDAKLVNRHISDVVP
                     RDLVCVNIDYGQMGVGGDTSWGARTHPQYCLTAREYKYGFRMVPLK"
BASE COUNT         2613 a         2534 c         3066 g         2427 t
ORIGIN      
        1 gaagtcattg cgtgaatttc gcacgcaagt gcaggcgggg aagcagaata agtgcaatta
       61 gtggtatatt tatcagcatt aaaaaaatcc aaagaatgaa aagagccacc gcccttctgt
      121 ttgcagcttt gctgttagtg tcagtcagcg cccagcaata tccgtttctt gatacgggac
      181 tttcatttga aaaacgggtt gatgaccttg tctcccggat gaccactgag gagaaagcct
      241 cgcagcttct ttatactgct cctgcaattc cccgcctggg cattccggaa tacaactggt
      301 ggaatgaggc acttcacggg gttgccagag cgggtcacgc tactgtcttt ccgcagtcga
      361 ttacttttgc gaacagttgg aatgaagagc tgatgcttga tgtggccaat gccatctcgg
      421 atgaggcaag ggcaaagcac catgagtttg tgagaagagg tgaccgcggc atatatcacg
      481 ggctgacctt ctggtctccg aacatcaatc tcttccgtga cccccgctgg gggaggggtc
      541 aggagactta cggtgaggac ccgtacctta ccgggaggct gggattacgg tttgtgcagg
      601 ggatgcaggg atctgatccg aaatatttta aaaccattgc aaccgccaag cattatgcgg
      661 ttcattcggg cccggagccg gtcaggcatg agttcaatgc ggtgatcagt gagcgtgacc
      721 tccgcgagag ctatctgcct gcgtttcgga cactggtcaa agaggggaag gtctactcaa
      781 taatgggcgc ctacaaccag ttcagagatc acccggcatg tgcaaatgat gagctttacg
      841 gcattctgcg aaatgaatgg ggttttgacg gttacatagt ctcagactgc tgggcggtat
      901 ctgattttta caatttccag gggtatgccg cgggcccggc tgaagcggct gcactggcgc
      961 tgaaagcggg aactgacctc gagtgcgggg tagactaccg ccacctgacg gaagcactga
     1021 agaggggact gataacggaa gaggatattg accgttcggt acgaagagtg atgctggcca
     1081 ggttcaggct cgggatgttc gatcctgaca gtatcgttga ttatgcgcag ataccctatc
     1141 aggcaaactg ctcagactac aaccggtcgc ttgccaggaa ggcagcccgg gaatcgatag
     1201 tccttctgaa gaacagcaac ggcacccttc ccctgagcag ggatatccgc acggtggccg
     1261 taatcggtcc caacgctgcc agccatgagg tactcgtggg gaattacaac ggcattccaa
     1321 agaacccggt aagcctgctt gaggggatag aggccaaact aaggcctgag actgagattc
     1381 tttatgccgg ggggagtgac ctcgcgcccg ggatgcataa cctggaacca ataccatcaa
     1441 gatatctgcg tacacctgac ggggcgcagg gagtgctggg cgcttactac acgtcacgtg
     1501 aaatgaaggg tgagcctctc ttcacccgta ccgatgacat gattgacttt tactgggaca
     1561 atgattcacc tcatccgcta atgccggtaa acgacttcgg cattcactgg gttgccgatc
     1621 tggttccacc ggtgacgggt acatattatc ttggaacatg gggttcatcg cgctatacaa
     1681 taatcctcga aggtgacacc ctctattcat tctacagtga ccatcatgcc aacatacggg
     1741 gagtgccggt tgatttcagg gcgggagaaa agtacagggt tgaggtgcta taccgcaacc
     1801 agggcggtga cgccgacatg aaacttcttt ggagcattcc aaaggatgac atgaaagcca
     1861 gagcggtgga ggcagcttca agggctgatg cggtaattgt tgcactcggt ctctcgcccc
     1921 gtctcgaggg ggaggaacag agactgaaac ttgagggttt cgaaggagga gacagaacca
     1981 ctctcggcct gcctgccatc caggaagagc tgctggcagc gctgctcgcc accgggaagc
     2041 cggttgtggt ggtgctgatg aatggcagtc ccgttgcttc cctgcaggcc catgagaaag
     2101 ccgcagccat gttgctggca ggttaccccg gggaggaggg tggcaacgcg atagctgatg
     2161 tcctcttcgg tgattacaat ccggcaggac ggctgcctgt cacctactac agatcggttg
     2221 accagctgcc tccttttgag gactatgata tggacaaccg cacttacagg tatttcaggg
     2281 gaacacccct ttatcctttc gggtacggat tgagttatac cacctttgcc tattcagacc
     2341 tcgtggtgcc ggcaaaggcc gtagcaggca cggaggtaaa ggtgagtgtg accgtcacca
     2401 ataccgggga aatgacgggt gacgaggtgg tacagcttta tctgactgat gagaaagcca
     2461 ctactccgag acctctcagg cagcttgaag gattcgaccg gctaaccctg aagccgggtg
     2521 agagcagaag ggttgagttt accctgaagc ctgagcagtt ctcaatgctc aacaataggg
     2581 atacaagggt tattgaaccc ggatggttta ccgtcagcgt gggaggcggg cagccgggag
     2641 cgatggtaca aggtgctgag gatcatgttc aggtcctgac cgcaagactc aggcttacag
     2701 ggggagaggt tgttgtgaaa gattaactcc ggcagcgaaa gtgatcagtc tgatgctggt
     2761 tcataaaccc gcggatcatt gagtaccgtg aggccatcat gatagccttt atcggtctcg
     2821 gggtgaatat cctcagcgcc tttctgctgc atcatgacca tgaagagagt gactacaata
     2881 tcagggcggc ataccttcac gtggtggctg acgcaattac cagcgtggca gccatcgtgg
     2941 gactgctcac tgcaatgatt tgggggctgc cctatgtaga tgcaatagca gccctcatca
     3001 gctcagcagt cattatcagg tggtcactca gactgctgag agactccggc agagagctta
     3061 tagcctgagg gttataaaaa atgttaaata ggttaaacat gataaatgtc atcatacgca
     3121 cattgattgg gacgtacatt tgctgacacc taaatagtgt acttcttaca ctttcgtcta
     3181 catactgatt tgtttcgaca aatcacgtcc tatcagacag tttaggtttt aggggttatt
     3241 ttacatgccg tcccgggtaa gcgggacggc attttttgaa tgtgcaggcc agacactctg
     3301 acgcaatact ccttttcagg ggttgccggg aaggcattct tttgtgcgcc cggcatgggc
     3361 gataactata gggtggaagt cccgaacgca ccctgacgca atactccttt tcaggggtca
     3421 ccgggacggc attttttgag tgtgaacggc atgggtgatt catcagttag caggattgat
     3481 ttcaatagtt atctttgccc ggaaacagta aaaaatgatg ttgcccccgg agtttcttga
     3541 gcggattgaa aggcaggcct atattgatgc cagtggtctt gctgaggctc ttggcgggcc
     3601 gtcggccgcc tcggtgcggg tcaacaggtt caagtggcct cacagggtaa gcggctatga
     3661 cagggtggag tgggagcctg atggatttta tcttcccggc aggcccctct ataccagtga
     3721 tccgctgttt catgcagggg tatattaccc gcaggagtcg tcagggatgt ttacaggaga
     3781 gatgttccgt caggtgactg ccgggcagga ggggctcagg gtgcttgatc tctgcggcgc
     3841 cccgggcgga aagagcaccc atcttgcctc cattctcgga gatgacgggc tgctggtagc
     3901 gaatgaggtc atcaggtcac gttctgccat tcttgcggag aacattacca agtggggaac
     3961 aggcaatgtc attgtgacaa atgctgatcc ttcgcgcttc gcgcaacttc ccgctttctt
     4021 tgatgtggtg gttgctgatg cgccctgttc gggcgaaggg atgttccgtt cacccgttgc
     4081 tgtaagggaa tggtcattgc agaatgcgaa tctatgctgt gaacgtcaga ggaggataat
     4141 aatggaggta tggcctgccc ttaaacccgg aggcatcctg atctattcaa cctgtacctt
     4201 caatcctgct gaaaatgagg agaatgttga atggataagg aagggtacag gcgctgaacc
     4261 tgtcatgata aaccttcctc cgggcagtcc cgtggttccg gtgcggtatg gaggtacaga
     4321 gggttacgga ttttatcccg gcagagtcag gggagaaggg tttttcattg ctgccatccg
     4381 aaagcctgtg acaccggctc aggacgagcg ggcgcctgat ggcagaaaaa cggccgttaa
     4441 gccttcgccg gaggcatttg aaaagaccat ctcacttgct gcctttgacc gtgaaagggt
     4501 ggttatgcat gagggccgca tcattgcact ggcggcagat gccggccttt tcggattcat
     4561 tgaggcaagg ctgccggtaa taaaggcagg aacgatgata ggtgaactga aaaagaatga
     4621 gctgataccg gcacatgacc ttgcaatgtc agcgaggcag aggcaggaag catggacgag
     4681 gcactcactc tcacgtgagg aagctttggc tttcctgagg ctggaggcgc tgaacggatc
     4741 cgcgatgcct gaggtgagag ttctgctgac ctatcgtgat gtgcctctgg gttttgtgaa
     4801 gaaccttggc aacagggtca ataacagcta tccccggttg tggcggatca ggatggaaag
     4861 agaaaccgta atgacggaga ttttataaat catgttgaac taagtaacag atcagtaaat
     4921 ttttgccaac agacacataa tcaattaatt ggagtgcaat ttttaagaaa ggcaattaaa
     4981 tctgacaacc aggcagttat aatcaatcat ttgaaaaaca tgccgaacca ttgactaaaa
     5041 aatcacaaca gataattgtt cttgtgagaa tgaattaaaa cctaattttg ccccaataat
     5101 tatttccctt atgaaaacag atatcgagat cgctcagagt gcctcaataa ggcctgtcgc
     5161 cgaggttgca gagcagctgg gacttaagga ggatgacctt gagttatacg gaaagtacaa
     5221 agccaaattg ccgcttaccc tcattgacaa gaaaaaggcg gcgcgtgcaa agctcatact
     5281 tgtgacagca attactccca caccttccgg tgaaggaaag accaccactt caatcggact
     5341 ggcacagggt ctgaacagac tgggcaagag agccaccgtg gtgctgaggg aaccctctct
     5401 gggacctgtt ttcggaataa agggaggggc agccggtggc gggtattcac aggtgatccc
     5461 catggaggac ataaacctcc attttaccgg tgatctgtct gccgtggaga aagcccacaa
     5521 tcttctttct gcaatgattg acaacaacat acagtcgaag gaaggcaatc tgggcattga
     5581 tccccggacc attgcctgga agagggtcat ggatatgaat gaccgtgctc tccgtgacgt
     5641 ggtgattggt ctgggcggaa cggcccaggg cgtgcccagg gagacaggat ttgatattac
     5701 tgctgcctcc gaggtgatgg caattctgtg cctggcaacg agcatcagtg atctcaaggc
     5761 gcggcttggg aatattttca tcggttacac atacgcaggc aaaccggttt ttgcacgtga
     5821 tatcagtgcc cagggtgcta tggccgcact ccttaaggat gccctgaagc ccaacctggt
     5881 ccagacgctc gaaggcactc ccgctattat tcacggtggt ccgtttgcaa acattgccca
     5941 gggtaccaac tcaatactgg caacaaggat ggggctttcg ctcagcgact atgtgattac
     6001 tgaggcaggc tttggcagtg acctgggcgc tgaaaaattc ttcaacatca agtcgcaggc
     6061 aggtaagctg agaaccaatg cggttgtgat agttgcaacc ataagggctc tgaagtatca
     6121 cggaggggtc agggtcccgg aacttgtcaa tgaaaacctt gaggcactga agagaggttg
     6181 cgagaacctt gacaagcatg ttgagaacat gcattattac gggttcaggc ctgttgtggc
     6241 aatcaaccgt ttcagttccg atacggatgc tgagattgag ctgctgaagg aatactgcga
     6301 agaacacaat gtcacggctg ctgtcaacac atcctgggct gacgggggga tgggagccat
     6361 tgagcttgcc aacgccgtca tcaaagcctc cgaagcatgc actgactgtt tcaccccgtt
     6421 gtatgaccat gcgtggccga tggagaagaa aattgagact atcgccaccc tgatgtacgg
     6481 tgcaagaaat gttgaataca ccctggaggc aaaggaacag ctcaaaaaga tttccgggct
     6541 tggacttgac aaacttgcgg tgtgcattgc caaaacccag aaatcgctct cagatgacca
     6601 gaccaggcgc aacaggccga gagacttcac cgtcagcatc cgtaaggtgg aactctcgtc
     6661 aggagcagga ttcattgtgc cgatagccgg gtctatcatg cgcatgccgg gtctgccggc
     6721 agtaccctcg gcagagaaga ttgatattga cgacaatgga ctgattagcg gattattctg
     6781 atcaaagcat ttcctgaaat atccgcagcc attcttgccg gcggacgctc ttcccgcttc
     6841 ggcggaagaa acaaggcgct gatacctgtt gatggtgtaa cggtcatctc gcgtacaatc
     6901 agccttcttg agcctctctt cagtgagata attgtagcgg gctggcctcc ggatggcccg
     6961 ctgccgggtg atgtcatact tgtgcatgac aggtacagtg gagtggggcc gctggccggc
     7021 atcgaggcgg cactgagggt ggccggctca ccactcctgt tcgtattcgg tggtgacatg
     7081 ccatggctct cggaagaact catcagggca caggtcaatg agtatattgc caatccttca
     7141 gatattcttg ttgcacggtc aggcggtctt gccgaaccgc ttcactcact ctacagccgg
     7201 aagatccata cggcactggt cagtcatatc gaagggggcg gcagcaatac catcattgat
     7261 ttctgcaaac ttgctgaaac ccgctattat gacatccccc tgaccctgga gacgcgtaaa
     7321 gccctgacaa acatcaacag gccggaagat ttgctgatat gatcctgttt tttcttattt
     7381 ttgataataa cataaagaaa tcagccatgc gaaattcact acttctcatg accctggcag
     7441 ctttgcttct gagctcttgc cagggatacc atgattacag caaaacggaa tggacggaaa
     7501 aggatttgcc ggaatgggaa gacctgactg tgaacacaat aaatactgtc acgccccatg
     7561 cctcgatggt aagtcatcct gacaatgctt ccgcactgaa ggcgaagtgg cgtgaatcgg
     7621 tcaatgtgct ttcgctcgac ggaaaatgga aattcaacta ttctccgaca ccagccgaac
     7681 gcccctattg gtttttcagg gaggattttg atacacgcca ttggggtgag attgaggttc
     7741 cttccacatg ggagagggaa gggcacggga cagcctatta tgtaaacgcg ggttacacct
     7801 tcccggtgaa tccgcccttc ataaaccatt cagataaccc ggttggctcg tataagcgct
     7861 catttacaat accgtcaggc tggaaggaca aggaggtctt tctcaacttt gacggtgtga
     7921 gttcagcctt caatgtctgg gtcaacggtg agaaggtggg ttacagtgaa gacagcaaga
     7981 caacagccga attcaatatc accccgttcc tcaggaagaa gaaaaacgat atcgctgtag
     8041 aagtttaccg ctactgtgac ggcagttacc tggaatgcca ggattttttc aggctgagcg
     8101 gcattaagcg gtcagtctgg ctgcatgcca gaccgaagac ctatatacgt gattatttcg
     8161 ccgtgccgac actggtcaat gagtacactg acggcctcct gaacctgacc gtcgagcttg
     8221 ccggaaatgg cgggaggcgg cctgccgcca tgcggctgga ggcagggttg tatgacggga
     8281 agacgaagat actcggtgag gccagggatg tgcaggcttt tgacagcctg gctgttgtga
     8341 aattcagcgc cgaagtgcca aaggtcaatc cgtggtctgc cgaatcaccc tcgctataca
     8401 cgcttgtcat tacccttgct gacaaccgcg gaagggtggt cgagagcatt tcatcgaaga
     8461 tagggttccg tacttccgag gtgaaaggag gacgcttcct ggttaacggg aaggcaatct
     8521 atctcaaggg gacaaatatg cacgagcatg acccggtgcg gggtcacacc atagacgagg
     8581 cattgatgct gaaggacata aagatgatga agatgaacaa tatcaacgcc gtgcgtacct
     8641 cacactaccc gcagcctgaa agattctatg agctgtgcga tgagtacggc ctttacgtca
     8701 ttgacgaggc aaatatcgag tcgcatggca ttggttacca ccctgacgtc accctggcac
     8761 acaagcccga gtggctcggg cagcacatgc ttcgtaccgt caggatggtg gagcgcgata
     8821 agaatcatcc ttcgataata atatggtcat tgggcaatga agccggtgac ggccagaact
     8881 tcgtcgagac ctacaagtgg atcaagggaa gggatgagag caggcctgtc cagtatgaaa
     8941 gagctgagaa gtcaacccac accactgaga gacatactga catctggtgc ccgatgtatc
     9001 cttccataga gagagttgag tcatatgccc agagtgaggg ccagcccggc ttcgaccgtc
     9061 cgtatataat gtgtgagtat gcccattcga tgggtaacag cacaggtaat ctgcaggatt
     9121 actgggatgt gattgaaaag tacgggattc ttcagggtgg ttttatctgg gactgggttg
     9181 accagggatt gcttgaaacc actgaggagg gggaggatta ttatgcatat ggtggtgatt
     9241 acggtgagcc gggatcaccc agcgacggca acttctgctg taacgggctg gtggggcctg
     9301 accgtacccc gcaccccgcg ctcaacgagg tctggaaggt ttaccagtat gtgggattca
     9361 agccggcgga ccttgccagg ggactggtca ccatttacaa taagtatgac ttcacaaacc
     9421 tttcatattt cacaatccac tgggaggtac tggccaacgg agctctcttc cgctccggca
     9481 atcttaagca gatcagcctc gagccgggtg actcgctcac cgtggagata ccctgggggg
     9541 agattgttcc ggcgtcggat accgagtatc atcttaacct gcatgtttca cgctccgatg
     9601 agtggggaat agtgccgcag gatcacatgt acgcatcgga acagatgctt ctgccgttcc
     9661 atgctgtgac agagaccggt acagcttccg gggctgccgg agcgccggat ggagagaccc
     9721 ttgaaatcat cgatacgcgg actgagggtt ctgacctgac attcgccgga gagggtttcg
     9781 aggtggtgtt tgacatggaa gccggggtga tgaaatcata cagggttgca ggtcaggaac
     9841 tgatcatgtc agggccccgt cccgacttct ggagagcccc cactgacaat gactatggca
     9901 acggcatgga gaagagaaac gcttcgtggc gggaggcagg cagcagggca gtgctgaaat
     9961 cagcccgcat cagccatttt gcaatgggca gcgcaggggt ggagttcacc ttcgacatcc
    10021 ccggtgaagg cgggatgaaa atagccgggc tggtgacagg gtacagggtt catgccggag
    10081 gttcggttga tgtatcgttc aacttcacca aaaccgatat gagcctcgag gagatcccca
    10141 gggtggggat gcagatgatc cttcccggga gtttcgttga cctctcctgg ttcggccggg
    10201 ggccgcatga aaattatgtt gaccggaaga cgtccgcctt tgttggtgta tatgaaagct
    10261 cggtggctga ccagtatgtg ccttacgtaa ggccgcagga gaacggatac aaaactgacg
    10321 tacggtggct gaatgtgacc gacggcagcg ggaagggaat ccgttttgag ggtgagcccc
    10381 tgttctcatt tgcagcgatg aattacctgc atgaggattt cgagtcgcag ggccgtctgg
    10441 caggatacag gcctgatgcc aaactggtca acaggcatat cagtgatgtt gttccgcgtg
    10501 acctggtgtg cgtcaatatt gactacggac agatgggtgt gggaggcgat acctcatggg
    10561 gtgcacgcac acacccgcag tactgcctca ctgcccggga atacaaatat gggttcagga
    10621 tggtgccgct gaagtagcag
//
LOCUS       DAOPBK010000089        10540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6130_length_10540_cov_6.159084, whole genome shotgun sequence.
ACCESSION   DAOPBK010000089 DAOPBK010000000
VERSION     DAOPBK010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6130_length_10540_cov_6.159084"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..949)
                     /locus_tag="PLA17_06525"
     CDS             complement(227..949)
                     /locus_tag="PLA17_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85106.1"
                     /translation="MNEIPIIKKGAIKDYWKNGFSGGLIIGILVFLLSLTNNSALTSL
                     YFGLSTWFGIIALWIGIGFTSEEYFKRKKKIKNLVSDKYAFLNQQNFTLHEDLYFEGV
                     YNGFFFRVLPMTKWIQRKRGRGNSIEYIVIESLYKSDSDSIDKEREAKMCGDYFLGKI
                     HFGHHCARFVPKDWDNPDFKENFDGLISIFKRENLIPLMKEDWENTFGKKLKKAKEDE
                     ENTKTVQILKIGKLDIKYKKPE"
     gene            complement(1073..1420)
                     /locus_tag="PLA17_06530"
     CDS             complement(1073..1420)
                     /locus_tag="PLA17_06530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPK85107.1"
                     /translation="MQVKRFEIWVADLNPQIGTEPGKTRPVLIVQTNLLNMVPHPSTI
                     ICPLTTSIQKESEILRVHIKKGTANLNESCDIMIDQLRAINNKRLLKKLGELPENLSD
                     KVKENLTIILDLY"
     gene            complement(1410..1640)
                     /locus_tag="PLA17_06535"
     CDS             complement(1410..1640)
                     /locus_tag="PLA17_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85108.1"
                     /translation="MKTISLKIDDSIFRETENILSRIKKPRNRYINEALEYYNKIQRR
                     KILESKLKKESALVKDESLSVLNEFEEIDYAG"
     gene            complement(2053..2655)
                     /locus_tag="PLA17_06540"
     CDS             complement(2053..2655)
                     /locus_tag="PLA17_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85109.1"
                     /translation="MMKRILIANALIILTSILYGQELKISIAPTINSGLYYQFVAGSP
                     GQNIKAGLTTSLDYLFLSDNKINFGLGCNYHFAQVEFVPNKNTADMPLHSERVNIISL
                     RLKSVLRFKNQWYFSLDPSVDFHLNYDAEQTLDKQSGIGLSCGLGKNIQIKEAIYLNI
                     EPRLWVHNIFSFTEQDLPYRLTALGLNLGLVFGSKSIDNK"
     gene            3221..4669
                     /gene="sufB"
                     /locus_tag="PLA17_06545"
     CDS             3221..4669
                     /gene="sufB"
                     /locus_tag="PLA17_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPK85110.1"
                     /translation="MENNQDKLLGEVTSGEYKYGFVTDIETDVIPKGLSEDVVRLISA
                     KKEEPEWLLEFRLKAFRRWQTMKMPEWAHLNIPEIDYQDIIYYAAPKQKKGPDSLSDV
                     DPELLKTFDKLGIPLEEQKRLTGVAVDAVMDSVSVKTTFRETLAEMGIIFCSFSEAVK
                     EHPTLVQKYLASVVPYSDNFFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINASNT
                     GQFERTLLIADEESYVSYLEGCTAPMRDENQLHAAVVEIVAERGAEVKYSTVQNWYPG
                     DKEGKGGIFNFVTKRGICKGDDSRISWTQVETGSAITWKYPSCILRGNNSVGEFYSVA
                     VTNNRQQADTGTKMIHIGSNTKSTIVSKGISAGFSQNSYRGLVKVLGKAEGARNFSQC
                     DSLLLGDRCGAHTFPYIEVDNPSAIVEHEATTSRIGEDQIFYCNQRGISTEDAIGLIV
                     NGYAREVINKLPMEFAVEAQKLLQISLEGSVG"
     gene            4777..5559
                     /gene="sufC"
                     /locus_tag="PLA17_06550"
     CDS             4777..5559
                     /gene="sufC"
                     /locus_tag="PLA17_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPK85111.1"
                     /translation="MLQIKDLKASIDNKEILKGLNLEVKHGEIHAIMGPNGSGKSTLA
                     NVLTGREIATVTAGTVTYEGKNLLEMSPEERAREGIFLSFQYPIEIPGVSMVNFMKTA
                     INEQRKYRGLEPLSASDFLRLMREKKELVEIDSALTNRSVNEGFSGGEKKKNEIFQMA
                     MLEPRLALLDETDSGLDIDALRIVANGVNKIRRPDTSVIVITHYQRLLDYIVPDYVHV
                     LYDGRIVRSAGPELALELEERGYDWIRKDADLSEETWTTDRK"
     gene            5538..6896
                     /gene="sufD"
                     /locus_tag="PLA17_06555"
     CDS             5538..6896
                     /gene="sufD"
                     /locus_tag="PLA17_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPK85112.1"
                     /translation="MDYRQEVTGRLTELWEGRETGILKGSPEIFNRYRTDAFSQFLRL
                     GIPDRKNEAYRYTNLEKSFSHKYEKYFSPKPDDFREAERFHCEVEDLDVWNMVLLNGF
                     FPRGEEGLAMLPGGIWVGSMRAAAKQFPSLVEKHYNKYAKNEKDGLVSLNTALATDGL
                     FIYAPGNTVNSKPIQIVNLLQSEQDFFSQDRNLLIADDGARMSLLVCDHAISPQHYLT
                     NTVTEIFVGRDASFEIIRVQNQHNYSGKITHTFIHQERDSYATSNNITLHGGLVRNAT
                     WHKLNGENAETHSYGLFLADRQQHIANFVKVDHVAPNCNSTQLFKGILDENATGAFNG
                     RIMVHKDAQNTNAYQSNNNIILSDTARMDTKPQLEIYADDVKCSHGATVGQLDENALF
                     YLRSRGIDAREAKLMLMFGFAHDVVKNIPIEALRDRIDHLVVQRLRGELSYCASCAIQ
                     CN"
     gene            6905..8131
                     /locus_tag="PLA17_06560"
     CDS             6905..8131
                     /locus_tag="PLA17_06560"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPK85113.1"
                     /translation="MKLTKSIEEIRADFPILSTKAHGKPLIYLDNGATTQKPLQVINL
                     MDELYRTTNANVHRGVHYLSDRVSERYEAARETVRAYINAGAREEIIFTSGTTASINT
                     VAFSFGERYIHEGDEIIISGMEHHANIVPWQMLCERKRAKLKVIPFSDEGVLDLEAYR
                     SLLTERTRIVAVTHVSNVLGTINPVKEIVAEAHRHGIPVLVDGAQSIQHGFVDVRDID
                     CDFYVFSGHKVYGPNGIGVLYGKADMLNELPPCQGGGDMVANVTFEKTTYNVIPFKFE
                     AGTTNFTGAIGLAAALDYLSALGREAVACREQSLLARATAGLSAIEGIRIYGTTPHKV
                     SVISFLPGTIHQYDTGMILDKMGIAVRTGTHCAQPLMERYGISGTVRASIAFYNTEEE
                     IEALVKGVRKVTEMFA"
     gene            8141..8569
                     /locus_tag="PLA17_06565"
     CDS             8141..8569
                     /locus_tag="PLA17_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HPK85114.1"
                     /translation="MNINEIQDQIIDEFGQFGDWMERYEYLIDLGRSLGPFDERHKTP
                     EYLIEGCQSKVWLYPSFENGIITFSAESDALITRGIVALLIRVFSGHTPAEIVDANIY
                     FIEKIGLRENLSPTRSNGLLAMVKQMRLYALAYKAKQEHS"
     gene            8566..8886
                     /locus_tag="PLA17_06570"
     CDS             8566..8886
                     /locus_tag="PLA17_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HPK85115.1"
                     /translation="MNTQEIQETEARIVGVLKNIFDPEIPVNIYDLGLIYEVRVDEEQ
                     IAHITMTLTTPNCPLAEDLVEEVRYKVAGIRGVKDCDLKLTFSPPWDRSMLSDEAKLE
                     LGLL"
     gene            8978..9916
                     /gene="queG"
                     /locus_tag="PLA17_06575"
     CDS             8978..9916
                     /gene="queG"
                     /locus_tag="PLA17_06575"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPK85116.1"
                     /translation="MTDERKIALTRKIRESLRMEGFDIYGIAQAAAHHEDNTCIAEWI
                     AAGKNGSMKFFERNLDKRGDITTLVEGARSVIVAGIRYYSNEPAAGKENYFVSRYARV
                     RDYHLVIKEKLARVQKIIKEEVPDAVSRAFCDAGPVTEKNWAVRAGLGWRGKHSLVIN
                     RDIGSFFFLGEIVTTAEFIYDSATSPDHCGDCTLCIDACPTGAICSDRTIDARRCIAY
                     LTIEHDGSIPEEFRGKTERIIFGCDRCQEVCPWNKKAVPYSDKELIPDFSVTSLTRKD
                     WHSLTPDEFDKIFRSSAVKRAGYEKIRRNIDFADHR"
     gene            complement(10200..>10540)
                     /locus_tag="PLA17_06580"
     CDS             complement(10200..>10540)
                     /locus_tag="PLA17_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK85117.1"
                     /translation="VGKNAILLVDFANDAIKEGKEINDALIQAVRIRTRPILMTALST
                     IIGMLPVALSKGSGAELRNGLAWVVIGGMMLSTFLTLIVVPVMYRILHSGQGRRGYRQ
                     KVDIERLMVE"
BASE COUNT         2883 a         2510 c         2524 g         2623 t
ORIGIN      
        1 atttgccagt tttcaaatca aaatattctt tgtcattgaa cttttcaaat ctagtctatt
       61 tacattttgc accatacttt aaatgagtgc caacggtaaa ttgtatgaga aggggcggat
      121 tgcgtgcttc gtccccgtct gctgacataa aagctgatgc ggagcagaat atttcaaaca
      181 accacttaag tgccaatgtt ttatgccgca tattcgcagc tgacttttat tctggtttct
      241 tatatttaat gtcaagtttg ccaattttca aaatctgtac tgttttagta ttttcttcat
      301 cttctttggc tttctttagt tttttgccaa atgtgttttc ccaatcttct ttcattaaag
      361 gaatcaggtt ttctctcttg aatatgctta tcaaaccgtc aaaattctct ttgaaatcag
      421 gattatccca gtccttaggt acaaatctgg cacaatggtg gccgaaatgt atcttaccta
      481 aaaaatagtc tccacacatc tttgcctctc gttctttatc aatcgaatca gagtcagatt
      541 tgtataagga ttcaatgact atgtattcta tgctattccc tctacctcgt tttctttgaa
      601 tccatttagt catgggcaaa actcgaaaga aaaacccatt ataaacgccc tcaaaatata
      661 aatcctcatg cagtgtgaag ttctgctggt taaggaaagc atatttatct gaaaccagat
      721 tctttatctt ttttttcctt ttgaagtatt cttcactggt aaatccaatt cctatccata
      781 gagcaataat tccaaaccat gtggacaatc caaaatataa agaagtcaaa gcactattat
      841 ttgtcaagga tagcaaaaaa actaaaatac caattatcaa acctccacta aatccatttt
      901 tccaatagtc ttttatggct ccttttttta ttattggtat ctcgttcatt gattcctctt
      961 tatattattg cagtgttgtt ttataagttt gctgttaact ctcctcttca gccggaagat
     1021 cctcgcgccg cggagcacca atgacatata ccggcctgtt gtgctgaatg ctttagtaca
     1081 aatctagaat gatcgtcagg ttttctttaa ctttatcaga cagattttca ggtaattccc
     1141 caagcttttt tagaagcctt ttattgttaa ttgctcgcag ttgatcaatc attatgtcgc
     1201 agctctcatt caaatttgcc gtaccctttt taatatgaac tcttaagatt tcagattctt
     1261 tctgaatact tgtagtcaga ggacatatta tagttgaagg gtgaggtacc atattaagaa
     1321 gatttgtttg aacgatgagc acaggtctgg tctttcctgg ttctgtacct atttgagggt
     1381 tcaaatcagc cacccagatt tcaaatcttt taacctgcat aatctatctc ctcgaattca
     1441 ttcagtacag aaagagactc gtcttttaca agggcagatt ctttcttgag ttttgattcc
     1501 aaaattttac gcctctgaat tttgttataa tattccagag cctcatttat gtacctgttt
     1561 ctgggtttct taatcctgga gagaatattc tcggtttccc ggaagatcga gtcgtcaatt
     1621 tttaatgata ttgttttcat aatacaagca ctacttcaca aatatacata ttatatgtat
     1681 atactgtaag tatatcaaaa ttacatttat catgtgtgtt ttgacattca gcacagcgtt
     1741 cggcggaacc gaggtcccgg agtggatgag ctcatgagcc acgaaactga gtgagtagaa
     1801 aataatgtgg cggtggtacc gagtcgtggt agcgcacgtt tttgcaccag agctgcgatg
     1861 caggggatat gattgttact ggtttattct cgcgttggca aaaaccgtga caccatgagg
     1921 gagtttccgg cggtagccag ggcaggccgc accgctggac acttgaccgt cctctttggc
     1981 cataaagttc gcaaagtgcg gagcaccaat gacatatacc gacctgttat ggttagttgt
     2041 taattcacat acttatttat tgtcaatgga tttagaacca aaaactaatc ccagattaag
     2101 tcctaatgct gttaatctgt aggggaggtc ctgttctgta aatgaaaata tgttgtgaac
     2161 ccagagcctg ggttctatat tgagatatat cgcttctttg atttgaatat ttttccccag
     2221 gccacatgat aaacctatcc cggactgttt gtcaagagtc tgttctgcat catagttaag
     2281 atgaaaatct accgagggat ccagactgaa ataccattga tttttgaacc tgagaactga
     2341 tttcagtcta agcgagatta tgttaactct ttcagaatga agtggcatgt ctgcagtatt
     2401 cttgtttgga acgaattcca cctgagcaaa atggtagttg cagccaagcc caaaatttat
     2461 tttgttatca ctaaggaata gataatccaa agaggttgtt aatcctgctt taatattttg
     2521 ccccgggcta ccggcaacaa actgatagta aagtccggaa ttaattgttg gagcgatcga
     2581 aatcttgagt tcctgtccgt aaaggatgct ggtcaggatt attaatgcat tagcaatgag
     2641 tattcttttc atcatgaggt tattgttaat aaagttggac taattatcta aagccattaa
     2701 gcaatctgtt ttaaccattt tgcattatta tagatatctt ttgtcatgaa agtaagatgg
     2761 caattaacta taatttgttt atatgtttaa gaaagaatta gataacctga ttgattcata
     2821 tgccctaaat taagtaaata aatcatatca atcattccag atgctttatt tcgaatcttt
     2881 ggtttagatt tccgtgttgt atcgggaaaa ggtcaaattc cggtgaggaa atgtcagttt
     2941 ttttgagcat tcatgaataa ttttggctta agatgtcggt agtgttccaa tcagtgtgtc
     3001 cggggtagca ggtcttcact gaagttttat atgagctata aggtccactt cttttcagca
     3061 ttggcaatag gattattccg gacaaactgt accacctgca accgcttcag atttcatatc
     3121 catatcgcca tttttgatta attttgcacg attctagaaa gatgtacaaa ctttaaccgg
     3181 tgacatttgt tgagaggatg taccgttgaa ggtgacgaat atggaaaata accaggataa
     3241 gctgctgggc gaagtaacat cgggagagta caagtacggc ttcgtgactg atatagaaac
     3301 cgatgtaatc ccaaaaggcc tcagtgaaga tgttgtgaga ctcatctccg ccaaaaagga
     3361 ggaacctgaa tggttgctgg agttccgcct aaaggcgttc cgccggtggc agacaatgaa
     3421 gatgccggaa tgggctcacc tcaatatccc ggaaattgac tatcaggata ttatttacta
     3481 tgccgcaccc aaacagaaga aaggtcctga cagcctgagc gatgttgacc ccgaactgct
     3541 gaagacattc gataagctcg gcatacccct cgaggaacag aaacgcctca ccggcgtggc
     3601 agtggacgcc gtaatggatt cagtatcagt aaaaaccacc ttccgcgaaa cacttgcaga
     3661 gatgggcatt atcttctgct cattctccga ggcggtaaaa gagcacccca cactggtgca
     3721 gaaatacctg gcctcggtgg tgccatatag tgacaacttc tttgccgcac tgaactcagc
     3781 cgtcttttcc gatggctctt tctgctatat cccgaaaggt gtcagatgcc cgatggaact
     3841 ctcaacatac ttccgtatca acgcatccaa caccggacag tttgaacgca ccctgctcat
     3901 cgccgacgag gagtcatacg tgagctacct ggagggttgc acagcgccga tgcgtgacga
     3961 aaaccaactc catgcagcag tggttgagat agttgcagag cgcggcgcag aggtcaaata
     4021 cagtaccgtg cagaactggt acccgggcga caaggagggg aaaggcggaa tattcaactt
     4081 tgtcacaaaa aggggtatat gcaagggtga cgattccagg atctcatgga cccaggttga
     4141 aaccggctcg gcaataacct ggaagtaccc ttcatgcatc ctccgcggca acaactccgt
     4201 gggcgaattc tactcggtgg ccgtaaccaa taatcgccag caggctgaca caggaacaaa
     4261 aatgatacac atcggcagca acacgaagag caccatcgtc tccaagggta tctcggccgg
     4321 attcagccag aacagctatc gcggactggt gaaggttctc ggcaaggccg aaggagcacg
     4381 caacttctcg cagtgtgact ccctccttct cggcgacagg tgcggcgccc acaccttccc
     4441 ctatatcgag gtagacaacc cctcggccat cgttgaacat gaagcaacaa cctcacgcat
     4501 aggcgaggac cagattttct actgcaacca gcggggcata tcaaccgagg atgccatagg
     4561 gctgattgtc aacggttatg cccgtgaggt gataaacaag ctgcccatgg aatttgctgt
     4621 ggaagcacag aaactcctgc agataagcct cgagggaagc gtgggctgat caccgggcat
     4681 tccggcttta tcgccataac aagctatatg acaataacct aaaaataaat accgcgcaac
     4741 agattcaaca gaacagagga tatgcaaacc gatttcttgt tacagataaa agacctgaaa
     4801 gcttcaattg acaataagga gatcctcaag ggtctcaacc tggaagtcaa acacggggag
     4861 attcatgcca tcatgggtcc caacggcagt ggtaagagta ccctggccaa cgtgcttaca
     4921 ggcagggaga tagcaaccgt tactgccggc accgtcacct atgagggcaa gaacctcctg
     4981 gagatgtcac ccgaggagcg cgcccgtgag ggaatattcc tctcattcca gtacccgatt
     5041 gagatccccg gcgtaagcat ggtaaacttc atgaagacgg ccattaatga gcagcgcaaa
     5101 taccgtggcc tcgagcctct gtcggcctct gacttcctgc gcctgatgcg cgaaaagaag
     5161 gagctggtgg agatagactc cgcactgaca aacaggagtg tcaatgaagg cttctccggg
     5221 ggagagaaga agaagaacga gatattccag atggcaatgc tggaacccag actggcgctc
     5281 ctcgatgaga ctgactcagg ccttgacatc gacgccctgc gcatcgtcgc caacggcgtg
     5341 aacaagatcc gcaggccgga cacatcggtg atagttatca cccactacca gaggctgctt
     5401 gactatatcg tacccgatta tgtgcacgtg ctctatgacg ggcgcatcgt aaggtcggct
     5461 ggccccgaac tggctctcga actggaggag agaggatatg actggataag gaaggatgca
     5521 gacctcagcg aagagacatg gactacagac aggaagtaac aggcaggctc accgaactgt
     5581 gggaagggag ggagacagga atactgaaag gatcgcctga aatattcaac aggtaccgta
     5641 cagatgcctt cagccagttc ctccgccttg gcattcccga caggaagaat gaagcctaca
     5701 ggtacacaaa cctcgagaaa tcattcagtc acaaatacga gaaatacttc tcgcccaaac
     5761 ctgatgattt cagggaggca gagagattcc actgtgaggt ggaggatctt gacgtatgga
     5821 acatggtgct gctcaacggc ttcttcccga ggggcgaaga ggggctggca atgctgccgg
     5881 gtggtatatg ggtgggtagc atgagggctg ccgcgaagca atttccatca ctggtggaga
     5941 agcattacaa caaatatgcg aagaacgaaa aagatggtct cgtgtcgctg aacaccgcgc
     6001 tggcaactga cgggctcttc atatatgcgc ccgggaacac cgtcaacagc aaaccgatac
     6061 agattgtaaa cctgctgcaa agcgagcagg actttttctc acaggaccgt aacctgctga
     6121 tagcagatga tggtgcccgg atgtcgctcc ttgtctgtga ccatgcgatc tcaccgcagc
     6181 attacctgac caacaccgtt actgagatat ttgtaggccg ggatgccagc tttgagatca
     6241 tcagggtgca gaaccagcac aactattcag gcaagataac tcacaccttc attcaccagg
     6301 agcgcgacag ctacgcgacg tcaaacaaca tcaccctcca cggcggactg gtgcgcaacg
     6361 caacgtggca caagctcaac ggagagaacg ctgaaacaca cagttatggc ctcttcctgg
     6421 ctgacaggca acagcatatt gccaacttcg tgaaggttga ccatgttgca cccaactgca
     6481 acagcacaca gctcttcaag ggtatcctgg atgaaaatgc caccggagcc ttcaacgggc
     6541 gaataatggt acacaaggat gcacagaata caaatgccta tcagagcaac aacaatatca
     6601 tcctctctga taccgcgaga atggatacca agccgcaact tgagatttat gctgacgacg
     6661 tgaaatgcag ccacggcgcc accgtgggcc agctggatga aaatgctctc ttctatctgc
     6721 gctcgcgcgg gattgatgcc agggaggcca aacttatgct gatgttcggc tttgcacacg
     6781 atgttgttaa aaacatacct attgaggccc ttcgcgatcg gattgaccac ctggtggtgc
     6841 agcgcctgcg cggcgagctt tcatactgtg cctcgtgcgc aatacagtgt aactgaacaa
     6901 cgtgatgaaa cttacgaaga gcatagaaga gataagagct gactttccca tcctttcaac
     6961 gaaagcacac gggaaacctc tgatttatct tgacaacggc gcaacaaccc agaagccatt
     7021 gcaggttata aacctgatgg atgagctcta ccgcacaacc aatgccaacg tgcaccgtgg
     7081 cgtgcattat ctctcagaca gggtttcaga gcgttacgag gcagcccgcg agactgtcag
     7141 ggcttacatc aatgccggtg ccagggaaga gatcatcttc acctccggaa ccaccgcatc
     7201 aatcaacact gttgcattct cgttcggcga gagatatatt cacgagggcg atgagattat
     7261 catcagcggc atggaacacc acgccaacat cgttccatgg cagatgctct gcgaacgcaa
     7321 gagggcaaaa ctgaaagtca tacctttctc agacgaaggt gttcttgacc ttgaagcata
     7381 caggtcgctg ctgacggaac gcacccggat agtggctgtc acacatgttt caaatgtgct
     7441 gggcacaatc aaccccgtga aggagatagt tgccgaggcc caccggcatg gtatcccggt
     7501 gcttgtcgac ggagcacaga gcattcagca cggatttgtt gatgtgagag acattgactg
     7561 tgatttctat gttttttccg ggcacaaggt ttacggccca aatggcatcg gcgtgctata
     7621 cggcaaggct gacatgctga acgagctgcc accctgccag ggaggcggtg acatggtggc
     7681 aaacgtcaca tttgaaaaga ccacatacaa cgtgatcccg ttcaaatttg aggccggcac
     7741 aacaaatttc accggcgcga taggactggc tgcagcactg gactacctct ccgcactcgg
     7801 ccgcgaggct gtcgcctgta gggaacagtc gctgcttgcc cgggcaactg ccggactgtc
     7861 agccatcgaa ggtatccgta tctacggcac taccccgcac aaggtctcag tgatctcatt
     7921 cctgccggga acaattcacc agtatgatac cggcatgatt cttgacaaga tgggtattgc
     7981 ggtcaggacg ggaacccact gcgcacagcc gctgatggaa cgatacggaa tcagcggaac
     8041 cgtaagagct tccattgcct tctacaatac ggaagaggag attgaggccc tggtgaaagg
     8101 tgtaaggaag gtgaccgaaa tgtttgcttg atataataaa atgaatatta acgaaataca
     8161 ggatcagata attgacgagt tcggccagtt cggcgactgg atggagaggt atgagtatct
     8221 catcgatctg ggcagatcac tcggcccgtt cgatgagagg cacaagaccc cggagtacct
     8281 gatagagggc tgccagagca aggtgtggct ctacccgtca tttgaaaatg gtatcatcac
     8341 tttttccgca gagagtgatg cactgatcac caggggtata gtggcgctgc tgatacgcgt
     8401 cttttcagga cacaccccgg cagagattgt tgacgcaaat atctatttca ttgagaaaat
     8461 cggccttagg gagaacctgt cccctacccg gtcaaacggc cttctggcca tggtgaaaca
     8521 gatgcgtctg tacgccctgg catacaaagc aaaacaggaa cactcatgaa tactcaggaa
     8581 atacaggaga ctgaagcacg catagttggc gtactcaaaa acatctttga ccccgagata
     8641 ccggtgaata tctatgacct cggcctcatc tacgaggtca gggttgatga ggagcagatt
     8701 gcgcacataa ccatgacgct aaccactccc aactgccctt tggctgagga tctcgttgag
     8761 gaggtcaggt acaaggttgc cggtatcagg ggggttaagg attgtgacct caaactcact
     8821 ttctccccgc catgggaccg cagcatgctg agcgacgagg ccaaacttga actgggattg
     8881 ctctgagaat tatgtagttt taccggtacg gggagagtgc tccgggttcc ggacactgac
     8941 cggctgacta ttctgaggct gcttttatat ccttaccgtg accgatgaac gtaaaatagc
     9001 gcttaccagg aaaatccgcg agagcctgcg gatggaaggc tttgatattt acggtattgc
     9061 acaggctgca gcccatcatg aagacaacac ctgcatagct gaatggattg cagccggtaa
     9121 aaacggttca atgaaatttt ttgagagaaa cctggataaa cggggagata taaccaccct
     9181 tgttgaagga gccaggtcag tgattgtggc cggaatacgc tactacagca atgaacccgc
     9241 agccggtaag gaaaactatt tcgtcagccg ctatgcacgt gtaagggatt accatctggt
     9301 aatcaaagaa aaactggcca gggttcagaa aataatcaag gaagaggttc ctgatgcggt
     9361 ttcacgggca ttctgcgatg ccggtcccgt tacagaaaaa aactgggccg tccgtgccgg
     9421 tctgggatgg cgcggtaaac acagcctcgt aatcaacagg gatatcgggt cattcttctt
     9481 cctgggcgag atagtcacca cagcagaatt catatatgac agcgcaacgt cacctgacca
     9541 ctgtggcgac tgcactctct gcatcgatgc ctgccccacc ggggccatct gcagcgaccg
     9601 gacgatagat gcacgccgtt gcatcgccta cctcacgata gaacatgatg gcagcatccc
     9661 tgaagaattc cgcggaaaga ctgaaaggat aatcttcggc tgtgaccggt gccaggaggt
     9721 ctgcccctgg aacaaaaaag cagtgcccta ttccgataag gagctgattc ctgatttttc
     9781 cgtcacctca ctgacaagaa aggattggca cagcctcacc cccgatgagt ttgacaaaat
     9841 cttcaggtca tcagccgtaa agcgggcagg ttatgaaaag atcaggcgga atattgattt
     9901 tgcagatcac cggtaactta gtagagaatt gaaattcgtg ccggggtgtg ggctgttccg
     9961 gggcgcaggt caaaatggac tctatcagct ccgctttgcc ggtcagtgat aatacaaccc
    10021 aacccggagg agccacccta aagcagcttg atctgcgccg gggcatggac cggctcgggt
    10081 tgcatgctat cctggagcgc gaaccacgct ggggtatgaa ccagactggg atgtggttcg
    10141 caccgatgcc cggttcgcgc cggggtgatc gcgccccggg gcagcctcca cgacagttgt
    10201 tattcaacca tcagccgttc aatatcaacc ttctgcctgt agcccctcct tccttgtccg
    10261 gaatgaagta tcctgtacat caccggcacc actatcaatg tcagaaatgt ggataacatc
    10321 atccccccga tcacaaccca tgccaggccg ttcctcagtt ctgcccctga tccttttgac
    10381 agggcaacag gaagcatccc gataatggtc gaaagtgccg tcatgaggat cgggcgtgta
    10441 cgtatccgca ccgcctggat gagtgcgtcg ttgatctcct tgccttcctt tatcgcatca
    10501 ttggcaaagt caacaagcag tatggcattc ttcccgacga
//
LOCUS       DAOPBK010000090        10468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6180_length_10468_cov_5.411409, whole genome shotgun sequence.
ACCESSION   DAOPBK010000090 DAOPBK010000000
VERSION     DAOPBK010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6180_length_10468_cov_5.411409"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLA17_06585"
     CDS             <1..571
                     /locus_tag="PLA17_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85118.1"
                     /translation="YERVLDKITRTNEFEFIEFTGKKQLRPFIIPILELMNDTFAEIY
                     GFVPLNDKEKKEFAARYLPILDPKFIKTVRKDGELIGFAIGMPDLSAGIKACRGRVLP
                     FGIFSILRESKRSKKLMMMLGGVRKDFRGKGIDVMMGVKILRDAINSRMKTIDSHLIL
                     EDNHRMRGEYERIGCKVVKKYRIYQKDLA"
     gene            complement(646..3177)
                     /locus_tag="PLA17_06590"
     CDS             complement(646..3177)
                     /locus_tag="PLA17_06590"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPK85119.1"
                     /translation="MKRWILYSISVLLSLNAWGQSCSILSKANNITPDKLCSPVTATW
                     TVSYTGVDDKGAPVAIRFDWNNGNIEILPATETSPGVFEATSINTYTSAGNICNYHPQ
                     ATLMVDGVLCTSSSQEQIVTVWDDDDHNGGHMHINPVVYPICFGNSANVRFQDLTQFN
                     CVPPQERDNPNVNTRWIQWIYGTDNTMTGAPVTVNGHNRTFPHVGNVITLTGPVTGSG
                     VWSDVLFVADDKLIGQYFEVTLRNWNYCNPYDDPNIPGAPDDRVNGDHPPVVTTARIL
                     IVPYPDATITPVAPMCDNASPVVLTAHDPGGTWSGSGVSGNIFDPSLAGPGSHTIRYE
                     IVNADGCRDEDQTSITVYPSPDATIITSGIVCSTDPVITLQANDPGGVWSGTGVSGNL
                     FYPAVAGSGNHLITYAITDANGCSDMDSAILTVATPDATITPVDTLCEDSPPVVLTAH
                     DLGGVWSGPGVVGNMFVPALAGTGNHIIKYEILNTDCAASDTAVVTVMPLPSIIIDDP
                     GTLFLNGPPVALTAYPTGGTWSGPGITGNMFDPSEAGLGNHMLTYSIPPERWGCSAQR
                     DVYVRVIMPPMPVADFEGATTGCAPLTVKFINKSQHGESYIWDFGDRQYSSEKNPVHT
                     YFVPGTYLVKLTVHNIAGESVRNDVVEVYQNPSAIFDAYPTNVVNNEQIVVFYSYSYF
                     AESWLWRFGDGKSSSEENPWHKYESPGSYTVSLIVSTREGCIDSAEMDTPVVVEWKEG
                     TLEFPNVFRWNGTGPTGGKWLEGVNPSMDEVFRPFFENIMEYRLQIFNRWGVLIYESD
                     DLKKGWDGYFGDGNLAPQGVYVWKASGRYADGEYFDMVGDVTFLH"
     gene            3325..5046
                     /locus_tag="PLA17_06595"
     CDS             3325..5046
                     /locus_tag="PLA17_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPK85120.1"
                     /translation="MKITVNDTLLELHSGATVKDALLSYYARSGRKLPGRLPTVEDRF
                     GNIVAPDGALSEGNCLIIRSGRKRKTSAARSVIVLLASAILFGCSSTRQTATSHSNEK
                     PDVTAVAPPSGIAASPERQAVIFSVNDMHAAIDNFPKLAWIVDSLRGVYPDLLLVAAG
                     DNQTGNPVNDQFPEKGFPVIDLMNAVGFNLSAVGNHEFDTGPEGFGNLISKADFSFIC
                     ANVSGGGRHPLKIDPYKIITLPNGLKVAFLGLLQLGQNGIPDSHPDNVRDFVFRSPLR
                     AAPEFLWLKDSSDVFIALTHIGFEEDVKLAETMPPGIDLIIGGHSHTRVDREQVHNGI
                     LITQADSKLKFGTLIRLKVDAAGRVSSSMQLIDIRNSKKERPEIRAMVDRYNDNPVLN
                     QVIATATDDFSSSEELGYLMADGQRAAAGADIALMNPGGVRIDRLPKGPVTIKNVYQL
                     DPFGNELVVTKLTGSEIFSLLRAAWSADRKSPLFTSGITTETRVNDHGEPEEITVHTD
                     DGKPLDLNHVYSVAMNSYMTQVYRYEHADPGQSLFLTTAQALIGYLKQEGNIKSYRGE
                     KRVRVTR"
     gene            5068..5715
                     /locus_tag="PLA17_06600"
     CDS             5068..5715
                     /locus_tag="PLA17_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPK85121.1"
                     /translation="MFHIVFDFDGTLADTLELGLNVYNRIAPEYDCQPVGEGEQELFR
                     TKRPQELLEAYGISRLKLLTLVMRIRKEINRHVPEMKLFDNMEKALRDIREAGFGLGI
                     LTSNSVENVRQFLELNNLTALFDFIYSGRNFLGKERVIRKMMIREQLHAERIVYVGDE
                     TRDIEACQAAGIPVIAVSWGLNRRELLASLSPDQIADRPEELPDRVRQVLLSRAE"
     gene            5838..7493
                     /gene="vsr"
                     /locus_tag="PLA17_06605"
     CDS             5838..7493
                     /gene="vsr"
                     /locus_tag="PLA17_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HPK85122.1"
                     /translation="MDVLTKEQRSKNMKAIRSKNTKMELLLAKALWAQGVRYRKNDKA
                     IFGCPDLTIRKHKLAIFVDSEFFHGKDWDREKYRIKTNREFWWKKIETNQKRDQTVNE
                     FLVNNGWKVLRFWSRDVHKKLNLCIYKINAVINEQNMVKYSEIRKKLNININKPELNE
                     LAHLTHFLQSYQNGSSSFFEKEALRYLHAVQFNADPLDEPYFQARLPIEWDIPFPPVE
                     HPKFTFIDLFAGIGGFRLAFQELGGKCVFSSEYNYFAQKTYEANFGEVPFGDITKIDE
                     KEIPDHDILIAGFPCQPFSIAGVTKKNALGRNHGFKDETQGTLFFDIARIIEEKKPKT
                     FFLENVKNLVSHDSGRTFMVISNTLKELNYTIYPKVLDGKHFVPQHRERIVIIGFSND
                     FFKGKEKFEFPDMPIADHRIVDILEKKADPKYTLSDNLWEYLIRYAEKHKAKGNGFGY
                     GMTDLNGISRTLSARYYKDGAEILIPQKNKNPRRLTPRECARLQGYPDTFVIPVSDNQ
                     AYRQFGNSVVVPLMKYVGRQLVSELLRHDKQYSPIRHTDSPRV"
     gene            7444..8619
                     /locus_tag="PLA17_06610"
     CDS             7444..8619
                     /locus_tag="PLA17_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HPK85123.1"
                     /translation="MTSSILQYAIQTAQESDISFSKFITANDTGTTGGHQSGYHIHKQ
                     AWQLFFESPGTKGKNSEKWITIKWQNDFETESRFIYYGVGTRNEYRLTRFGRGFPFLS
                     DDNVGDLLVICRKSQDYYEAFVIQRDEEIEMFFASLNLTINDTNKIIPKTGLLLPEDR
                     MFKCFSDYIKSLKVDFPPALELSNNARQCFNSAYDITQTDILNNPDKNLLSWLNAEFQ
                     LFKIIETDRYSARIRTPFLSVGELVEIANKVLNRRKSRAGKSLENHLAEIFHQFNLSF
                     ETQVVTEGNKKPDFIFPSQEAYLNPEFDSDKLKVLASKTTCKDRWRQVLNEADRIKTK
                     HLFTLQQGISSNQLEEMLKYHVQLVVPSLYIKSFPEQYRPHIMTLGSFVNYIKGIQD"
     gene            complement(8712..9122)
                     /locus_tag="PLA17_06615"
     CDS             complement(8712..9122)
                     /locus_tag="PLA17_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85124.1"
                     /translation="MKKKINLPENHIRSLSSSLRMVEDSLVELEVMLLNQSSSCCSVV
                     HKDVDEETIESCIAIIQEAKDYICELSEKYGTLKENLSLQRIINAKRTIIWGLLKDSL
                     SRRMKGYGTFPKEHAGEYDADINRLIEITNKLNC"
     gene            complement(9534..10382)
                     /locus_tag="PLA17_06620"
     CDS             complement(9534..10382)
                     /locus_tag="PLA17_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009752460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85125.1"
                     /translation="MMNTKRTFDEIQQSELGKYVYALIDPRDSKIFYVGQGTSNRVFD
                     HFNDADKLNPQKIDLRKASKKLLRILDIWENDLDVDWIIIAYGLDKSVEDLDLASRLE
                     SGVYHCLEQSINGHLLNDNTPPKSSLLLPEDVEALGAEYVNPEEEIHNVFIFPIHAAL
                     IAGASPYDATRRAWLISKEFQNLGTSYAVGLKNSISKGSYKVNKWIDSELSPGRFEFL
                     APGHPNPPTLDSLLNKNWTNVLSVVKGYWQRGNYVIVDFDGRGHFRIKRGSVVKTIWY
                     DCLPPK"
BASE COUNT         2947 a         2337 c         2404 g         2780 t
ORIGIN      
        1 gtatgaaagg gtcctcgaca aaataaccag aaccaatgag tttgaattta tcgagttcac
       61 aggcaagaag caattgagac cctttatcat tcccatcctc gaactgatga atgacacatt
      121 cgccgagata tacgggttcg ttccgctgaa tgacaaggag aaaaaagagt tcgcggcacg
      181 ctacctcccg atacttgacc ccaagtttat caagacggtg aggaaggatg gagagctgat
      241 aggatttgcc atcggaatgc ctgacctgag cgccggtatc aaagcctgca ggggaagagt
      301 tctcccgttc gggatattca gtatcctcag ggagtcaaaa cgttcgaaaa agctgatgat
      361 gatgctcggt ggagtcagaa aggatttcag gggcaaaggc attgacgtaa tgatgggcgt
      421 gaagatactc cgtgatgcca tcaacagccg tatgaagacc attgacagcc accttatcct
      481 tgaagataac caccggatga gaggtgaata tgaacggatc ggctgcaagg tggtcaagaa
      541 atacagaatt taccagaagg acctggcgta aataaccagg tgattcttta tttttcagtc
      601 atttccgccg gatagtcacc accccggcag aggcggccgg tggttctagt gcaggaaggt
      661 cacatccccc accatgtcaa agtattcgcc atcggcatac ctgcctgagg ctttccagac
      721 ataaacaccc tgtggggcca ggttgccgtc tccgaagtaa ccgtcccacc ccttcttcag
      781 gtcatcgctc tcatagatca gcacacccca cctgttgaag atctgaagcc tgtactccat
      841 gatattctca aaaaacggcc tgaaaacctc atccattgac ggattcacac cctccagcca
      901 tttgccgccg gtagggccgg tgccattcca cctgaagaca ttcggaaact ccagggttcc
      961 ctccttccac tcaaccacca cgggtgtatc catctccgct gaatcaatac acccctcacg
     1021 ggtggaaaca atcagtgaga cagtataaga cccgggcgac tcatatttgt gccagggatt
     1081 ttcctcagag gagctcttcc cgtcaccgaa tctccagagc catgactccg cgaagtagga
     1141 atagctgtag aaaaccacta tctgctcatt gttgaccaca ttcgtgggat aggcatcgaa
     1201 tatggcagaa ggattctggt aaacctctac aacgtcgtta cgaaccgatt caccagcgat
     1261 gttgtgaacg gtgagcttca ccaggtacgt tccgggaacg aaataagtat gcacagggtt
     1321 tttttcggat gagtactgtc tgtcaccgaa atcccagatg tagctctcgc cgtgctggct
     1381 cttgtttatg aacttaacgg tgagcggggc gcatcccgtg gtggctccct cgaaatcggc
     1441 aacaggcatc ggaggcataa ttacccttac atatacatcg cgctgtgctg agcatcccca
     1501 tctctcggga ggaattgaat aggtaagcat gtgattgcca agcccggcct cagacggatc
     1561 gaacatgttg ccggtgatgc ccggcccgga ccatgtgcct cctgtggggt aggctgtcag
     1621 cgccaccggc ggtccgttga ggaagagagt tcccgggtca tcgattatga tcgagggcag
     1681 cggcatgact gtcaccactg ccgtgtcaga cgcggcgcag tcagtgttca gtatctcata
     1741 tttgataata tggttccccg tgccggcaag agcgggcaca aacatgttgc ccacaacccc
     1801 cggccctgac cagacccctc ccaggtcatg tgctgtcaga acgactggcg gactgtcttc
     1861 gcagagggta tccacaggtg tgatggtagc atcaggcgtg gcaacggtga ggatcgccga
     1921 atccatatca ctacagccgt tggcatctgt gatggcgtat gttatcaggt ggtttcctga
     1981 tccggctact gccgggtaaa agagatttcc cgagacaccc gtgcctgacc atacccctcc
     2041 cggatcattc gcctgaagtg taatgaccgg atcggttgaa cagacaatgc cactggtgat
     2101 tatagtggca tcgggactgg ggtaaacggt aattgatgtc tgatcttcat cccggcatcc
     2161 gtcggcatta acaatctcat acctgatggt gtggctgccg ggcccggcca gtgaggggtc
     2221 aaatatgttg ccgctcaccc cgctcccgct ccatgtgcct cccggatcat gagccgtcag
     2281 gactaccggt gacgcattgt cacacatcgg ggctaccgga gtgatggtag catcagggta
     2341 gggtactatc aatatccttg cggtggtgac aaccggcgga tggtcaccgt tgacacggtc
     2401 atcaggagcg ccaggaatgt tgggatcatc gtatggatta cagtaattcc agttacgaag
     2461 ggtaacctca aaatactggc ctataagctt gtcatctgct acaaaaagga catcggacca
     2521 gactccggaa cctgtcaccg gacccgtaag ggtgataaca ttgccaacat ggggaaatgt
     2581 cctgttgtga ccgttaacag taacaggagc accggtcatg gtgttgtctg taccgtagat
     2641 ccactgtatc cacctggtgt tgacgttggg attatccctc tcctggggag gcacgcagtt
     2701 gaactgcgta aggtcctgga aacggacgtt ggcgctgttg ccgaaacaga tcgggtagac
     2761 caccggattg atatgcatat ggcctccgtt gtgatcatca tcatcccata ccgtcactat
     2821 ctgttcctgc gatgatgagg tgcacagcac cccgtcaacc attagtgtgg cctgtgggtg
     2881 gtagttgcag atatttcctg ctgaggtata ggtgtttatt gatgttgctt caaacacacc
     2941 cggtgaggtt tcagtggcag gcaggatttc tatgttgcca ttgttccagt caaaccttat
     3001 tgccacaggc gcccctttgt catcaacacc cgtatagctt acggtccagg tggcggtcac
     3061 cggtgaacaa agcttgtccg gagttatgtt gttggccttg gacagaatcg agcaactctg
     3121 cccccaggca ttcaacgaca ataatacaga aatactatat aggatccatc tcttcatgaa
     3181 ccatctgact acatacccct cgccggtgtg taatacaaat tgttaaacgg ttataaagcc
     3241 ctcagggtta caattttttg cctgcagagt tttagcttgc aggaaaaaat gtattttgag
     3301 accgttattt caatactctg acgaatgaaa attacagtga atgataccct gcttgaactg
     3361 catagcggag ccacggtaaa agatgcactt ctcagttatt atgcccgttc cgggcgaaaa
     3421 ttacccgggc gtctccccac ggttgaagac cgcttcggaa acatagtggc tcctgatggc
     3481 gcgctgtcgg aaggaaattg cctgattatc agatcaggac gcaaacgtaa gacctcggcc
     3541 gccaggagtg tcatagtgct tctggcctcc gcgatcctgt ttggatgcag cagcacgaga
     3601 caaacagcca catcacattc aaatgaaaag ccggacgtaa cagccgttgc gccaccgtca
     3661 ggtatcgctg ccagccctga acgccaggct gtgatcttct cggtcaatga catgcatgcc
     3721 gccatcgaca acttcccgaa actggcctgg attgtggaca gcctcagggg tgtttatccg
     3781 gacctgcttc ttgtcgccgc aggcgataac cagaccggca atccggtgaa tgaccagttc
     3841 cctgagaagg gctttccggt gattgacctg atgaatgccg tcgggttcaa cctcagcgca
     3901 gtggggaacc acgagtttga taccggcccc gaaggattcg gcaacctgat cagcaaggca
     3961 gatttcagtt ttatctgtgc caatgtatcc ggcggtggcc ggcatcctct gaaaatcgac
     4021 ccttataaaa tcatcaccct tcccaacggg ctgaaggtgg cattcctcgg cctcctgcaa
     4081 ctcggacaga acgggattcc tgacagccat cccgacaacg tcagggattt cgtgttccgc
     4141 tcacccttaa gggccgcccc tgaatttctc tggctgaagg atagcagtga tgttttcatt
     4201 gccctcacgc atatcgggtt tgaggaggat gtgaaactgg ctgaaacaat gccgccgggg
     4261 attgatctta tcataggagg gcattcacat accagggttg acagggaaca ggtccacaac
     4321 ggcatcctga ttacacaggc tgacagcaag ctgaaattcg gcaccctgat cagactgaaa
     4381 gttgacgcag ccggccgggt cagcagcagc atgcaactga ttgatatccg gaactctaaa
     4441 aaggagaggc ctgagatccg cgccatggtc gacaggtaca atgacaatcc tgttctcaac
     4501 caggtgatag caacagccac agacgatttc tcatcatctg aggagctggg atacctgatg
     4561 gccgatgggc agcgtgcggc cgccggtgct gacatagcgc tgatgaaccc ggggggagtg
     4621 cgtatcgaca ggcttcccaa aggcccggta acaatcaaaa atgtctacca gcttgatccg
     4681 ttcggcaatg agctggtggt gacaaaactc accggcagcg agatcttttc actgttgcgc
     4741 gcagcctggt ctgcagacag aaagtcacct ctcttcacct cgggaatcac tactgaaacc
     4801 agggtcaatg accatggtga accggaagag atcacagtgc acaccgatga cggcaaaccg
     4861 ctcgatctga accatgtcta ctccgtcgcc atgaacagct acatgaccca ggtctacagg
     4921 tacgagcatg ctgatccggg ccagtcgctc ttccttacca ccgctcaggc gctgatcggt
     4981 tacctgaaac aggaaggcaa catcaaaagc taccggggag agaaaagagt cagggtaacc
     5041 aggtaattca ccctgtgatt gaataaaatg tttcatatag tttttgactt cgacggtacg
     5101 cttgccgata ctctcgaatt gggattgaac gtctataacc ggattgcccc ggaatatgac
     5161 tgccagcctg tcggcgaagg ggaacaggag ttgtttcgga cgaaaaggcc ccaggagctg
     5221 cttgaagcct atggtatctc caggctgaag ttactgacgc tggtaatgag gatcaggaaa
     5281 gagataaaca ggcatgtgcc tgaaatgaag cttttcgaca acatggaaaa ggctcttcgc
     5341 gatatccgcg aagcaggttt cgggttgggt attttaacct ccaactccgt ggagaatgtc
     5401 aggcagtttc ttgagctcaa caatctgacg gctctgtttg attttatata cagcggcaga
     5461 aacttcctcg gtaaggagag ggttatcagg aagatgatga tcagggaaca gctccatgca
     5521 gaaaggatag tttatgttgg ggatgaaacc cgtgacatcg aggcatgtca ggctgccggc
     5581 attccggtca ttgctgtctc gtggggattg aaccgcaggg agctccttgc ctccctctcc
     5641 cctgatcaga ttgctgacag gccggaagag cttcctgacc gtgtgaggca ggttcttttg
     5701 tcaagggcag aatgaactac tgtcatacaa tactcccctg aattaagacc actggttaag
     5761 gctatatttg acatctttaa accagttaat aacaaagagt gcttgacttt accgtattta
     5821 accttatatt tatcattatg gatgttctga cgaaagaaca acggtcaaaa aacatgaaag
     5881 ctatcaggag taagaacaca aagatggaat tacttctggc aaaagctctt tgggctcagg
     5941 gcgttagata caggaagaat gataaagcaa ttttcggatg tccagattta acaataagaa
     6001 aacataagtt ggcaatcttc gtggatagcg aattctttca cgggaaagat tgggatagag
     6061 aaaaatatcg tattaagacc aatagagaat tctggtggaa aaagatagaa acaaaccaga
     6121 aaagagacca gacagtaaat gaatttcttg taaataatgg ctggaaagtt ttacgatttt
     6181 ggagcaggga tgtccacaaa aaattgaacc tttgtattta taaaataaac gctgttatca
     6241 acgaacaaaa catggttaag tattccgaaa tacggaagaa attgaatatt aatattaata
     6301 aacctgagtt aaatgaactg gctcatctga cccatttttt gcagtcgtat cagaatggaa
     6361 gttcttcttt ttttgagaag gaagcattga ggtacttaca tgcagtgcag tttaatgctg
     6421 atcctcttga tgagccttac ttccaagcca ggcttccaat agaatgggac attccctttc
     6481 cgccagttga acatcccaag tttacattta ttgacctatt cgctggtatt ggtggattta
     6541 gacttgcatt tcaggaattg ggaggtaaat gtgtcttttc aagtgaatat aattacttcg
     6601 cccaaaagac ttatgaagct aattttggtg aggttccatt tggagatata acaaaaattg
     6661 acgaaaaaga aatccctgac catgacattc ttattgcagg atttccctgt cagccgtttt
     6721 ctattgcagg ggtaacgaaa aagaatgcac tgggtagaaa tcatggattt aaagatgaaa
     6781 cacaggggac tttattcttt gatatagcca ggataataga agaaaagaaa cctaagactt
     6841 ttttccttga aaatgttaag aatcttgttt cgcatgattc agggagaaca ttcatggtga
     6901 tttccaatac cttaaaggag cttaattata ccatttatcc gaaagtatta gatggtaaac
     6961 attttgtgcc tcaacacagg gagcgaatcg taattattgg attttctaat gattttttca
     7021 aaggaaaaga aaagtttgag tttcctgata tgccaatagc tgatcataga atagttgata
     7081 tacttgagaa gaaagctgat cccaaataca ctctgtctga taatttatgg gaatatctta
     7141 tcagatatgc tgagaaacat aaggcaaagg gtaatggttt cggatatgga atgactgatt
     7201 taaatggcat ttcaagaaca ttaagcgcaa ggtactataa ggacggagct gaaattctta
     7261 tcccacagaa aaacaagaac ccaaggcgat taactccaag agaatgtgca agattacagg
     7321 gatatccgga tacttttgtt attcctgttt cagataatca ggcatacagg cagtttggta
     7381 attctgtagt agtgccactt atgaaatatg taggtagaca attagtctct gaacttctca
     7441 gacatgacaa gcagtattct ccaatacgcc atacagacag cccaagagtc tgacatatcc
     7501 ttctccaaat tcattacggc caatgacaca ggaacaactg ggggtcatca gtcgggatat
     7561 catattcata agcaggcttg gcagctattt tttgaaagtc cggggacaaa aggtaaaaat
     7621 tctgaaaaat ggattacaat taaatggcaa aatgattttg aaacagaaag cagattcatt
     7681 tactatggag ttggtaccag aaacgaatac agactgacaa gattcggcag gggctttcca
     7741 tttttatctg acgataatgt cggcgacctc cttgttattt gtaggaaatc acaagattat
     7801 tacgaagcgt ttgtcattca aagagatgaa gaaattgaaa tgttctttgc ctctttgaat
     7861 ctgacaatca atgatacaaa taagataatt cctaagacag gtctattgct acctgaagac
     7921 aggatgttta agtgtttcag tgattacatt aaatcactca aagttgactt tcctcctgca
     7981 ctggagcttt cgaataatgc cagacaatgt ttcaattctg cttatgatat tacccaaacc
     8041 gatatactaa ataatcctga caagaattta ttaagttggt taaatgctga atttcagctt
     8101 tttaagataa ttgaaaccga caggtattct gccagaatca gaacaccctt cttatctgtt
     8161 ggtgaattgg tagaaattgc aaataaggtt ttaaacaggc gcaaaagcag agcagggaaa
     8221 tcattagaaa accatcttgc agaaatcttt catcagttca atctatcctt tgaaactcag
     8281 gttgttactg agggcaataa aaaaccggac tttatctttc caagtcagga agcatattta
     8341 aatcctgaat ttgatagtga caagctgaaa gtattggcct ccaagacaac ttgtaaagat
     8401 cgatggcgtc aggttttaaa tgaggccgac aggataaaga ccaagcattt attcactctt
     8461 caacagggca tctcctctaa tcaattggaa gaaatgttaa aatatcatgt gcagttggta
     8521 gttcccagtt tatacatcaa aagttttccg gaacagtacc gcccgcatat tatgaccttg
     8581 ggtagttttg tcaattacat taagggtata caagattagg tatttagtta tgaatgactt
     8641 tctttgataa tttcaaatat agtttaatcc ctaactgtca gattaagttt tgactaattg
     8701 atgttaagtg attagcaatt caacttgttt gtaatttcaa tcagtctgtt tatgtccgcg
     8761 tcatattccc cggcatgttc ctttgggaag gtgccgtagc ctttcattct ccttgagaga
     8821 gagtctttca atagtcccca gatgatggtt cgttttgcat taatgattct ttgaaggctc
     8881 agattttctt ttaaagtgcc gtatttttct gataattcac agatataatc cttcgcttcc
     8941 tgaattatag caatacacga ctctatcgtc tcttcatcaa catccttatg cacaacactg
     9001 cagcaactgg agctttgatt caacagcatc acctccagtt ccaccaggga gtcttcaacc
     9061 atccgcaggc tcgatgacag ggatcttatg tgattctcag gcagattaat cttctttttc
     9121 attttatgta tcgacagaat ggtttgtaaa ggtattgaaa atctatctct tatctcctgg
     9181 tttttatgat gtctggtaac gggtcatcaa gaacaggact tgaaccgatg cgagcgtgaa
     9241 gaaaatgccc ggtgggcatt tttagcgaag cagtcagcct gcagggcggg cgttcaagtc
     9301 atagcaataa aaaaaccggc ctgtgaccgg ttttgctccc cagccagaac ttgaacctgg
     9361 gaccccctga ttaacaggca aatatgttgg tttccacagg tttatacaga tttgtatctg
     9421 tttgtaccaa ttacttttgt agcaacttaa ttgtaaaaga tttcaccgat ctatcccgat
     9481 atagtcaaaa tactggcccc gaaactggca ccagatttct ttaaatacgg aattcatttt
     9541 ggtggtaaac agtcatacca aatagtttta accacggaac ctctctttat tctgaaatga
     9601 cctcttccat cgaaatcaac aataacatag ttacctctct gccaataacc tttaacaact
     9661 gaaagcacat ttgtccaatt cttatttaaa agagaatcta aggttggtgg attgggatgt
     9721 ccaggggcta gaaattcaaa tctgcctgga gacaattcag aatcaatcca tttattaact
     9781 ttataactcc ccttagaaat agaattctta agacctacag cataggaagt ccctaaattt
     9841 tgaaattctt ttgaaataag ccaggctcgc cttgtagcat cataaggtga tgcccctgca
     9901 attaatgctg catgaatagg gaatatgaaa acattgtgaa tttcctcttc tggatttaca
     9961 tactcggctc caagtgcttc aacatcttcg ggaagtaata gtgatgattt tgggggagta
    10021 ttatcattta ataaatggcc atttatagat tgctctaaac aatggtatac tccagattca
    10081 agacgacttg caagatcaag gtcctctaca ctcttatcta atccataagc aataataatc
    10141 cagtccacat ctaagtcatt ctcccaaatg tcaagaattc ttagcaactt cttgctcgcc
    10201 tttctcagat ctattttttg agggtttaac ttatctgcat cattgaaatg atcaaatact
    10261 ctattgctcg ttccttgacc aacatagaat attttactat ctcttggatc aatcaaagca
    10321 taaacatact tgccaagttc tgattgttgt atttcatcaa atgtcctttt tgtattcatc
    10381 atttgattta agtggttcta ctgcgaattt aatggaattt gagatctttg gcaaaaaaga
    10441 tggtttatga tgttttcaga gcagtttt
//
LOCUS       DAOPBK010000091        10369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6249_length_10369_cov_5.046151, whole genome shotgun sequence.
ACCESSION   DAOPBK010000091 DAOPBK010000000
VERSION     DAOPBK010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6249_length_10369_cov_5.046151"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLA17_06625"
     CDS             <1..351
                     /locus_tag="PLA17_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85126.1"
                     /translation="SKTIREWAKQKKRHLTAAAYYKSQDKLRLFIEPFSRVSWYGLLI
                     AMLAMLISWPVVLFLALSRFVMRAVILKKAAAVFNERRLWFISLFFDIFAPFLTAFLY
                     LTNKIKGKGRTSWK"
     gene            342..941
                     /locus_tag="PLA17_06630"
     CDS             342..941
                     /locus_tag="PLA17_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK85127.1"
                     /translation="MEVESGLSDKAQYDLKVIDRALHGDSRAYTELLNRYRDSVYYVI
                     LRMVNNPSDAEDLTIEAFGKAFHNLAKYVPSHAFSTWLFRIATNNCIDFMRRKSQSPR
                     PFNQEEGEEDEVEATVASDMIAPDELMINRETAASLNRIVKTLKPRYRRLIELRYFEE
                     YSYEEIAKELSLPIGTVKARLFRAKVLILNMVQSKSSGI"
     gene            938..1600
                     /gene="rsmG"
                     /locus_tag="PLA17_06635"
     CDS             938..1600
                     /gene="rsmG"
                     /locus_tag="PLA17_06635"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPK85128.1"
                     /translation="MTTLTEKYFPSLTTLQRNQFSLLPEVYREWNNKINVVSRKDIDN
                     FEVNHLLHSLAIARFIRFAPGTSVIDVGTGGGLPGIPLAIFFPDVKFTLVDSIAKKIR
                     VASAVATEAGLNNVTTVTARAESLSGQYDFIIGRAVTETAQFIRLTRHLASRKSFNEK
                     KNGYILLKGGDLEKELRPFLKSVEIAEISKWFEEPWFGTKKVVYMPFSGIPLGSCSRS
                     TS"
     gene            1757..1915
                     /gene="rpmH"
                     /locus_tag="PLA17_06640"
     CDS             1757..1915
                     /gene="rpmH"
                     /locus_tag="PLA17_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPK85129.1"
                     /translation="MKRTFQPSRRRRANTHGFRKRMSTPNGRRVLAARRARGRKRLTV
                     SDEVSHKS"
     gene            2018..3127
                     /locus_tag="PLA17_06645"
     CDS             2018..3127
                     /locus_tag="PLA17_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HPK85130.1"
                     /translation="MKELVIKIADPELRELASAVTDGKRLTPAEALTLYRKADLSLMA
                     LLATTVKRKKSGDAVFFNRNFHIEPSNICVYNCRFCSYHKAKGDPESWEMTENEILDT
                     VRQFDGIPVTEVHITGGVHPSRDIYYYARLLTSIRQIRPDISIKAFSAVELDYMIREA
                     GMTYTEGIGLLHEHGLDSIPGGGAEIFDPELRRRICPEKADAETYLAIHETAHRLGLN
                     SNATMLYGHAESPEQRIDHMLRLRELQDRTGGFDAFIPLKFRSGNNSMSGLGEVPVTE
                     DMRNYALSRIFLDNFSHIKAYWPAIGRNSAMISLAFGTDDLDGTIDDTTKIYSMAGSD
                     EERPSLSTAELVAMIRSAGFRAVERDTRYNRVKEW"
     gene            3198..4055
                     /locus_tag="PLA17_06650"
     CDS             3198..4055
                     /locus_tag="PLA17_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPK85131.1"
                     /translation="MSVKEFFKSRVFWKQVGLAMVIGVAIIIVLIIWLNIYTRHGQAR
                     PTPEVRGLTITEAQHVVSKNRMKLQIIDSVYTTMVPRGCVAEQTPLPGHRVKKGRTIK
                     ATINAFNPEMVAVPDLVGLPRRQALSMIETAGLQVGQLNYVADLTVDFVLKQTIHGRE
                     ALPGDSVQKGMVIDLVLGRGLSSQRTSVPRLTGLTLDEARNEILGASLNLGAFVFDGT
                     VITRDDSLSAFVFKQNPEYHDEASVQLGSAVYIWLTTDSLKMPADTITADILLPEGDF
                     GQPDTGIPR"
     gene            4058..5914
                     /locus_tag="PLA17_06655"
     CDS             4058..5914
                     /locus_tag="PLA17_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85132.1"
                     /translation="MKKSRVIFVLIAALLTLDVSAQEVITGLFRNRQVSDAVRPASLL
                     KGAMADDPVSLPFSDDFTGDSIFPSAARWADQQAFINNTYSVRQPSAGIATLDCLDEN
                     GLLYERASKAVFPADRLTSRAIDLEYQPSDSIFLSFLYEAGGVADLPEEGDSLTLSFW
                     APEEQKWHSIWRTMGEVTDGFKMVIIPVTDTRFLMKGFRFMFTNYASLAKASTEPSQA
                     GNADQWNIDHVLLDKGRSVHDTVLRDVALTLPLRSLVKEYESMPWRHFRQAYLSAMSS
                     VAPVNYRNNDTIVRNVTRHITITDMSTNTVVRDFDAGAANALPLSDVTYDAPLLYTFN
                     TSSSADTAQFMVTISLITDDFDPKQNDTLRYIQKFSDYFAIDDGTAEAGYGINGQGAN
                     NAMAALRFRSFLPDSVTGISICFNDAYDNANQRAFDIMVWADDNGRPGALLGSSEGPV
                     ASPAGEINGFVTFMFDTPVRVTDNFWIGWRQHSDKFLNAGLDLNTAPAGRQYCHFSGK
                     WQESQAPGIIMMRPVMKGSGIPNSSDNGTLINNLFSLYPNPTDGLVTLVPAEGAPDDF
                     TIDVISSSGALVMSSGKTLRPDLSRLAAGNYMLLVRTRDGRPLSFLRVVKVK"
     gene            5924..7069
                     /locus_tag="PLA17_06660"
     CDS             5924..7069
                     /locus_tag="PLA17_06660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPK85133.1"
                     /translation="MSTEDFSGTGEQEKYNESDQEGQKQELYENFSDNGEQEEYNEPD
                     QEDQEQELYEHYRYVVDPGQSMLRIDKYLSRRMENVSRTRVQAAARAGNILVNDSPVR
                     SNYRVKPLDLIQVLLPNPPREIELIPQDIPISVVYEDDDLIVVDKAAGMVVHPAYGNY
                     SGTLMNALMYHFRDLPLFQTGDIRPGLVHRIDKNTSGLLVVAKNEYAHNRLAGQFFRR
                     TTGRLYTALVWGTPDPAKGTITGHVGRNVRDRKIMQVFPDGSQGKHAVTHYRIIEPLG
                     YVSLIECRLETGRTHQIRVHMAWKGHPLFNDEEYGGHRILKGTTFTKYQQFVRNCLDL
                     LPRQALHARTLAFDHPVTGRRLSFESPLPQDMVAVIEKWRNYISGRE"
     gene            complement(7080..7928)
                     /locus_tag="PLA17_06665"
     CDS             complement(7080..7928)
                     /locus_tag="PLA17_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPK85134.1"
                     /translation="MNRFRLYTMVLFWVLAALLVQAQTLVPRPDPPRLVNDLAGVLSS
                     GEVYALEKKLVAFNDSTSNQIAILIVNDLQGYDRSVFAFRVANDWGVGQGDFNNGVLV
                     LVKPKTPSSDGQVFIAIGAGLEGAIPDLAAADIIDREIIPRFRKNDYYGGLDDGTDVL
                     MSLAAGEYSYDRYARGGIPPVVIPIVALFLITLFITFIIRFAIGKANIKHIKHSGSSN
                     LPYWLYMGPIDGDDSHSGSWGGFSGSSGGSSGGSSSSFSSSSSFGSSGGFGGGSFRGG
                     GAGGRW"
     gene            complement(7931..8383)
                     /locus_tag="PLA17_06670"
     CDS             complement(7931..8383)
                     /locus_tag="PLA17_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPK85135.1"
                     /translation="MKASAFFTPEQQEVIIRSIREAEHSTSGEIRVHIVTSCKVDVLD
                     EAAWLFRKIGMHKTADRNGVLIYLALKERNFAIIGDSGINSVVPPGFWNDVRDHMQER
                     FSQSLFTEGLSEGILMAGEKLKEHFPRSKDDINELPDSISFDANEKGV"
     gene            complement(8394..8993)
                     /locus_tag="PLA17_06675"
     CDS             complement(8394..8993)
                     /locus_tag="PLA17_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPK85136.1"
                     /translation="MKKSLSGVAIIVIIVVVLLLIVSWGVGIYNKIVPLNEKVASEWS
                     NVESQYQRRADLIPNFVNTVKGAAAFEQETLTNVIEARAKASQITVDPSNITPEQLQE
                     FQAAQGEISSALQRLMVVVERYPELKATQNFRDLQVELEGTENRILMARGAFNDAARV
                     YNTFIKQFPNSFIASLTKFKERPYFEAQEGTEVVPKVEF"
     gene            9148..10116
                     /locus_tag="PLA17_06680"
     CDS             9148..10116
                     /locus_tag="PLA17_06680"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPK85137.1"
                     /translation="MRTIAIVAGGNSSEYPVSVRSAKGVESALEGKYETYIVNIKGTD
                     WYREDARGRVYRIDKNDFSLVLDDQRIRFDAAFIAIHGTPGENGLLQGYFDMIGMPYT
                     SCDAFVSALTFNKQACKLYLHEYGIPMTGGIVIRKGDKYDADGIVRQTGLPCFVKPNA
                     SGSSFGVTKVKETAEFAEALEKAFAEGHEVLVESFMKGTEVACGVVRTRERKIIMPVT
                     EVVPKKEFFDYEAKYTEGLADEITPARISEELTHRIQELSSEIYDILGCRGIVRVDFI
                     ITHNGPQFLEINTIPGMTPESIIPRQAAVFGISQADLYSMVIEDLF"
BASE COUNT         2663 a         2760 c         2539 g         2407 t
ORIGIN      
        1 tctaaaacca tccgtgagtg ggcaaaacag aagaaaagac atcttactgc cgcggcttat
       61 tacaagagtc aggataagct acggctgttc attgagccct tctcaagagt atcatggtac
      121 ggactgctga ttgccatgct ggcaatgctg atctcatggc cggttgtact cttccttgcc
      181 ctgtcgaggt ttgtcatgag ggcagtgata ctgaaaaagg cagctgctgt tttcaatgag
      241 cggcgtttat ggtttatatc gctctttttt gatatcttcg ccccattctt aacggcattc
      301 ctttacctta caaacaaaat aaagggtaag gggagaacat catggaagta gaaagcggcc
      361 tgtcagataa agcacagtat gaccttaagg taatcgaccg tgcactgcac ggcgacagca
      421 gggcttatac cgagctgctg aaccgttacc gtgactcggt ctactacgtc atcctgagaa
      481 tggtaaacaa tccctccgat gcagaggatc tgaccataga agccttcgga aaggcgtttc
      541 ataaccttgc caaatacgtg ccgtcacatg ccttcagtac atggcttttc aggattgcca
      601 ccaacaactg tattgatttc atgaggcgca aaagccagag tcccaggcca ttcaaccagg
      661 aagaaggaga agaagacgag gttgaggcaa cagtggcttc tgatatgatc gctcccgatg
      721 aactgatgat caacagggag acagcagcct cactcaaccg gatagtgaaa accctcaagc
      781 cgcgctaccg caggctgatt gagcttcgct acttcgagga gtactcctat gaggagatag
      841 caaaggaact gagccttccc attggcaccg tcaaagccag gctcttcagg gcaaaagtcc
      901 tgatactgaa catggtccaa agcaaatcaa gcggcatttg accacactga ccgaaaaata
      961 cttcccgtca ctcaccactc tgcagcgcaa tcagttctca ctcctgcctg aagtatacag
     1021 ggagtggaac aacaaaatca atgtcgtttc ccgaaaggat atcgacaact tcgaggttaa
     1081 ccacctgctt cactccctcg ccattgcccg tttcatcagg ttcgcgccgg gcacatccgt
     1141 cattgatgtc ggcactggcg gaggactccc gggaataccg cttgccatat tcttccctga
     1201 cgttaaattc accctggttg actccattgc caagaagatc agggtagcct cggctgtcgc
     1261 cacggaagcc ggcctgaaca atgtcacaac agtgacggca agggcggaat ctctttcggg
     1321 gcagtatgat tttatcatcg gcagggcagt gacagaaaca gcacagttca tcaggctgac
     1381 acggcatctg gcctcgcgaa aatcattcaa tgagaagaag aacggatata tactcctcaa
     1441 gggaggagac cttgagaagg agctcagacc attcctgaag tctgtcgaaa ttgcggagat
     1501 cagcaaatgg ttcgaagaac catggttcgg gacaaaaaag gttgtgtaca tgcccttctc
     1561 aggcattccc cttggttcat gcagccggag tacctcgtaa gtctcccggt tgtctgcctc
     1621 tttatctgag gcattccccc ggtaacgggg aaatgcttaa tattaaaaaa agtagccatc
     1681 tggcttcttt atttaaaaaa atgactacct ttgcatccgc aaaaaccggt tagttaacaa
     1741 caaaatatag catcagatga aaaggacatt tcagccatcg agaaggagaa gagcaaacac
     1801 tcacggcttt cggaagagaa tgtcaactcc caatggcagg cgcgttcttg cagcacgcag
     1861 ggcaagggga aggaagagac tcacggtttc agacgaagtt agccacaaaa gctgaaaggc
     1921 tgacagacaa tagataatag ccgtcttgat gaataattaa ggcggttttt tatttctgtt
     1981 atgcctccct gagttatttt tacaggaaat ctgaagaatg aaagagctgg taatcaagat
     2041 tgcagaccct gagttaagag aactggcatc agcagtgaca gacggcaaac ggctcacacc
     2101 cgctgaagcg ctcactctct accggaaagc cgatctttct ctgatggcgc tgcttgctac
     2161 cactgtcaag agaaaaaaga gcggcgacgc cgtctttttt aacaggaatt ttcacattga
     2221 gccttcaaat atctgcgtct ataactgccg cttctgttca taccataagg ccaagggtga
     2281 tcctgagagc tgggagatga ctgaaaatga gatacttgat acagtccgcc agtttgatgg
     2341 catacctgtc accgaggtcc atataaccgg aggggtgcat ccctcgcgtg acatatatta
     2401 ttacgcccgt ttactcacct ccatacggca gatccgccct gacatcagca tcaaagcctt
     2461 ttccgccgtg gagcttgatt atatgataag ggaggcaggc atgacttata ccgagggtat
     2521 agggttgctt cacgagcatg gccttgactc cattcccggc ggaggggcag agatattcga
     2581 cccggagctg cggcgccgga tatgccctga aaaggctgac gctgaaacct accttgccat
     2641 ccacgagacc gcgcacaggc tcggactgaa ctccaacgcc accatgctct acggtcatgc
     2701 cgagagccct gaacagcgca ttgaccacat gcttcgcctg agggagctgc aggaccgcac
     2761 cggaggcttt gatgccttca tcccgctcaa gttcaggtcc gggaacaaca gcatgtcagg
     2821 gcttggtgag gtgcctgtta cggaggatat gcgcaactac gccctgtcac gaatcttcct
     2881 cgataatttc agccatatca aggcatactg gccggcaata ggccggaaca gcgccatgat
     2941 aagccttgcc ttcggaactg atgacctcga cggaaccatt gatgacacca caaaaatata
     3001 ttcaatggca ggttcggatg aggagaggcc gtcactatca accgcggagc tggtggcaat
     3061 gatacggtca gccggtttca gggcagtgga acgtgacacc agatacaaca gagtgaagga
     3121 gtggtagaaa tccctccttc ccgcgatata atttttatct ttgaggtacc gttaataaac
     3181 taatacttca tcatacgatg agcgtaaagg aattcttcaa aagccgggtc ttctggaaac
     3241 aggtgggtct ggcaatggtc atcggtgttg ccattattat tgttctcatt atctggctta
     3301 atatctatac ccgccatggc caggcaaggc ccactcccga ggtcagggga ctgaccatca
     3361 cggaggcaca gcatgttgtc agtaaaaaca ggatgaagct gcagataata gactcggttt
     3421 ataccacaat ggttccacgc ggctgtgtgg ccgaacagac tcctctcccc ggtcaccggg
     3481 tcaagaaagg acgtacaatc aaggccacca ttaacgcctt taatcccgag atggttgcag
     3541 ttcctgacct ggtaggactg ccccgccgcc aggcgctctc aatgattgag acagccggtc
     3601 tgcaggtggg acagcttaac tacgtcgctg acctgacggt cgattttgtt ctcaaacaaa
     3661 caattcacgg tcgcgaagca ttacccggcg actccgtcca gaaagggatg gtaatcgacc
     3721 tggtgctggg ccgtggtctg agcagtcagc gcacatccgt tcccagactc accggcctga
     3781 cacttgatga agccaggaat gagatactgg gagcctccct caaccttggc gcctttgtct
     3841 tcgacggtac ggtgattaca cgcgatgatt cactcagtgc tttcgtcttc aaacagaatc
     3901 ccgaatacca cgatgaggca tcggttcagc tgggatctgc cgtttatata tggctcacca
     3961 ctgacagtct caagatgccg gcagacacaa tcactgctga tatcctgctc cctgaaggtg
     4021 acttcggaca accggatacc ggaataccac gctaaagatg aaaaagagcc gggtcatatt
     4081 cgttcttatt gctgccttac ttaccctgga tgtgtcagca caggaggtca ttaccggcct
     4141 tttccgcaac agacaggtct ctgatgccgt cagacccgcc tctctgctta agggtgcaat
     4201 ggctgacgac cccgtgtctc tgcccttcag cgatgacttt acaggtgaca gcatttttcc
     4261 ttcagctgcc aggtgggctg accagcaggc atttatcaat aacacctact ctgtcagaca
     4321 gccctctgcc ggtatagcaa cccttgactg ccttgatgaa aatggattgc tgtacgagag
     4381 ggcgtcaaag gcggtcttcc ctgctgaccg gctcacgtcg cgcgccatcg acctcgaata
     4441 tcagccttct gacagtatct tcctcagctt cctttatgag gcaggaggag tggcagacct
     4501 gcctgaggaa ggtgacagcc tcacactcag cttctgggcc ccggaagagc agaagtggca
     4561 cagcatctgg aggacaatgg gagaagtcac cgatggattt aaaatggtga taattcctgt
     4621 cactgacacc aggttcctga tgaaaggatt ccggttcatg ttcaccaatt acgcctcact
     4681 tgcaaaggcg agcaccgaac cttcacaggc aggaaacgct gaccagtgga acattgacca
     4741 cgtactgctt gacaaaggac ggtcagttca tgataccgtc ctgcgtgatg ttgccctcac
     4801 ccttcccctg agatcactgg taaaggaata tgagtcaatg ccgtggcgcc atttcaggca
     4861 ggcataccta tcagccatga gttcagtggc tccggtaaat taccggaaca atgacaccat
     4921 cgtccgcaac gtcaccaggc atatcactat cacagacatg tcaaccaata ccgtggtgcg
     4981 cgactttgac gcaggagcag ccaacgccct gcctctgtcc gatgtaacct atgacgctcc
     5041 gctgctctat accttcaata cctcctcatc agcagacacc gcgcagttca tggtgacaat
     5101 ctcactgata acggacgact tcgaccccaa acagaatgac accctgagat acatccagaa
     5161 gttttctgac tatttcgcaa ttgacgacgg aacggcagag gcgggttacg gcatcaacgg
     5221 tcagggcgca aacaatgcca tggctgccct ccggttcagg tctttcctgc ctgactccgt
     5281 taccggcata agcatctgct tcaatgacgc atatgacaat gccaaccaga gggcatttga
     5341 tataatggtc tgggctgacg acaacggacg ccccggcgca ttgctgggca gcagcgaagg
     5401 ccccgtggct tctccggcag gggagataaa cggtttcgta acattcatgt ttgacactcc
     5461 ggtgagggtg actgacaact tctggattgg ctggaggcag cattccgata agtttctcaa
     5521 tgccggatta gaccttaaca ccgccccggc aggaaggcaa tactgccatt tcagcggcaa
     5581 atggcaggag agccaggctc cgggtataat aatgatgcgg ccggtgatga aaggaagcgg
     5641 catacccaat tcatcggaca acggcaccct gatcaataac ctcttctcac tctatcccaa
     5701 tcctactgac ggcctggtaa ccctggtccc ggcagaaggg gcgcctgatg acttcaccat
     5761 tgacgttatc tcatcttcag gagccctggt gatgtcgtcc ggaaagaccc tgagacccga
     5821 tctgagccgg ctggccgccg ggaactatat gctcctggtc aggacaaggg atggacggcc
     5881 actgtcgttc ctgcgggttg taaaagtcaa gtgaggcagg aggatgagta cagaggactt
     5941 ctccggtacc ggtgaacagg aaaaatacaa tgaatctgac caggaaggcc agaagcagga
     6001 gctgtacgag aacttttccg acaacggcga acaggaagaa tacaatgaac ctgaccagga
     6061 agaccaggag caggagctgt acgagcatta tcgctacgtt gttgatcccg ggcaatcaat
     6121 gctccggatc gacaagtatc tttccagacg gatggagaat gtatcccgca ccagggtaca
     6181 ggcagcggcc cgggccggga acatcctggt aaatgactcc cccgtcaggt caaactatcg
     6241 ggtcaaacca cttgacctga tccaggtgct tcttcccaat ccgccccggg agattgaact
     6301 tataccacag gatatcccca tctctgttgt ttatgaagat gatgacctga tagttgttga
     6361 caaggccgca ggtatggtgg ttcacccggc ctacgggaac tattcgggca cacttatgaa
     6421 cgccctgatg tatcacttca gggacctgcc gctcttccag accggagata tccgtccggg
     6481 actggtgcac cgcattgaca agaacacatc gggactgctg gttgtagcca agaacgagta
     6541 tgctcacaac aggcttgccg gtcaattctt cagacggacc acagggaggc tttacacagc
     6601 cctggtatgg ggtactcctg atcccgcgaa agggaccatt accggtcatg tcggccgtaa
     6661 tgtacgtgac cgcaagatca tgcaggtatt tcctgacggc agccagggta agcacgcggt
     6721 gacccattac aggattattg aaccgctggg gtatgtttca ttgattgaat gccgccttga
     6781 gaccggccga acccatcaga taagggtgca tatggcctgg aaggggcatc cgctcttcaa
     6841 cgatgaggag tatggcggtc accgaatact taaaggcacc acctttacaa aataccagca
     6901 gtttgtccgc aactgccttg atctgctccc gcgacaggct ctccatgccc gcaccctcgc
     6961 ctttgaccat cctgtcaccg gcaggcgcct ctccttcgag tcacccctcc ctcaggatat
     7021 ggttgccgta attgagaagt ggaggaacta tatctcaggc agggaataat cccgcttttc
     7081 taccatcttc ccccggctcc tcctcctctg aaacttcctc cgccaaagcc tccgctgctg
     7141 ccaaagctgc tactactgct gaagctgctg ctgcttcctc cgctgcttcc tccgctgctt
     7201 ccggagaatc cgccccagct tccgctgtga gaatcatctc catctatcgg acccatataa
     7261 agccagtacg gcagattcga agatccgctg tgctttatgt gcttaatgtt ggctttgcct
     7321 atagcaaacc tgataatgaa tgttataaag agagttatca ggaataaagc cacgatgggt
     7381 atcactaccg gaggaatgcc gccgcgggca tatctgtcat agctgtactc gcctgcagca
     7441 agtgacatca gcacatccgt accgtcatca agcccgccgt aataatcatt cttcctgaac
     7501 ctgggtatta tctccctgtc aataatgtct gcggctgcca gatcagggat agccccctcc
     7561 aaaccggcac cgattgctat aaacacctgc ccgtcagatg acggggtctt gggttttacc
     7621 agcaccagca ccccgttatt gaaatcaccc tgcccgaccc cccagtcgtt ggccaccctg
     7681 aatgcaaaca cggaacggtc atatccctgg agatcattca caataagaat ggcaatctgg
     7741 tttgatgtgg agtcattgaa agcaacaagt tttttttcaa gagcatacac ttccccggaa
     7801 gagagaacgc ctgccagatc gttgaccagt ctcggcggat caggtcgtgg taccagtgtc
     7861 tgtgcctgga ccagaagtgc tgccaaaacc cagaacagca ccattgtata tagcctgaat
     7921 ctattcattg tcatactcct ttttcatttg cgtcgaacga gatgctgtca ggcagctcat
     7981 tgatatcatc ctttgatctg gggaaatgtt ccttcagctt ctcccctgcc ataagtatac
     8041 cctctgagag tccttcagtg aagaggctct ggctgaatcg ctcctgcata tggtccctga
     8101 catcgttcca gaacccgggc ggcacaaccg aattgatccc tgaatctccg ataatcgcaa
     8161 aattgcgttc tttcagcgcg aggtagatga gtacgccgtt acggtctgcc gtcttgtgca
     8221 tcccaatctt cctgaagagc catgctgcct cgtcaaggac atcaaccttg cacgaagtca
     8281 caatatggac gcgtatctca ccggaggttg aatgctcggc ctcacgtata gacctgatga
     8341 taacctcctg ctgttcaggt gtaaaaaaag cagacgcttt cataaccggg atgtcagaat
     8401 tcaacctttg gcactacctc agtaccctcc tgagcctcaa aatagggcct ctctttgaac
     8461 ttggtcagcg atgctatgaa gctgttcggg aattgcttta tgaaggtgtt gtacacgcgg
     8521 gctgcatcgt tgaaggcgcc cctcgccatc aatattcggt tttcggttcc ttcaagctcc
     8581 acctgcaggt cacggaaatt ctgggtagcc ttcagctcgg gatacctctc cacaacaacc
     8641 atcagtctct gaagggctga gcttatttcg ccctgagcgg cctgaaactc ctgtaactgc
     8701 tcaggcgtaa tgtttgacgg gtcaacagta atctgggagg cctttgccct tgcttcaatc
     8761 acattggtaa gcgtctcctg ctcgaaggcg gcagcaccct tgaccgtatt cacaaaattg
     8821 ggaatcaggt cagccctccg ctgatactgc gactccacat tgctccactc agaggcaacc
     8881 ttctcattca acggaactat cttgttgtaa atgcctactc cccaggagac tatgagaagc
     8941 aggacaacca caataatcac aattattgct actccgctaa gactcttttt catttttctt
     9001 tctgtttgat tacgtctcaa aaataaacaa aatattgcct taaggagttg cctggccttc
     9061 tctttactgc agtgccggac cagtttttta tcttcttgtg ccggcacgaa aataattgta
     9121 gtaattttgg acaaaacttg acgaaccatg agaaccattg ccattgttgc cggagggaac
     9181 tcatctgaat accctgtctc cgtacgcagc gccaaaggtg ttgaatcagc tcttgagggg
     9241 aaatatgaaa catacattgt caacatcaaa ggtaccgact ggtaccgtga agatgccagg
     9301 gggcgcgtct acaggataga caagaatgac ttctcactgg ttcttgatga tcagcgcata
     9361 cgtttcgatg ctgccttcat cgccatccat ggcaccccgg gtgaaaacgg actcctccag
     9421 ggttattttg acatgatcgg aatgccttac acgagctgtg atgcattcgt ttcggcactc
     9481 accttcaaca agcaggcatg caagctctac ctgcatgagt acggtatccc gatgaccggc
     9541 ggaattgtca taaggaaagg agataagtat gatgcggatg gcattgtcag gcagacaggg
     9601 cttccctgtt ttgtcaaacc caacgccagc gggtcaagct tcggggttac aaaggtgaag
     9661 gagactgccg aatttgccga agctctggag aaggcattcg ccgaaggcca cgaagtgctg
     9721 gtggagtcat tcatgaaagg aaccgaagtt gcctgcgggg ttgtcaggac aagagagagg
     9781 aagatcatta tgcctgtgac agaggtggtt cccaaaaagg agtttttcga ctatgaggca
     9841 aaatataccg aaggactggc tgatgagata accccggcac gtatttccga agagctgacc
     9901 caccgcattc aggagctctc atctgagata tatgatatcc tcgggtgccg tggcatcgtc
     9961 agggttgatt tcatcatcac gcacaacggg ccgcagttcc tggagataaa caccatcccg
    10021 ggcatgaccc cggagagtat aatcccacgc caggcagcag tgttcggaat cagccaggca
    10081 gatctctatt caatggtgat agaggatctg ttctgacacc ccattcttta gcgtataaag
    10141 aactgtacct ccctgccgcc ccggacacac tgattggatc actacccgaa gaagataagc
    10201 aaaccattct aaggctgata gaaaaaaatg cttaccaata acaaattcaa agacttcttt
    10261 cagaaaagcg ttgctgctct ataaatcaaa aagcccgggc cagccgggct tctttattat
    10321 tataattttc cttgattctg taacatcttc aatatccatc ctaagttaa
//
LOCUS       DAOPBK010000092        10219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6363_length_10219_cov_5.473731, whole genome shotgun sequence.
ACCESSION   DAOPBK010000092 DAOPBK010000000
VERSION     DAOPBK010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6363_length_10219_cov_5.473731"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..3423)
                     /locus_tag="PLA17_06685"
     CDS             complement(334..3423)
                     /locus_tag="PLA17_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPK85138.1"
                     /translation="MKKSSLSLVLVFCALIISASPVRQTASPARQADSPVRQTASPAR
                     QTDSPVRETDNNVPWKSFIGHPGVDSILNTMTIREMIAQLIWVPAWADEKGGNYRQVE
                     LLVEKYGIGGVIFFEGTREMQVDYTKRLRSISRIPLIIAQDAEWGTGMRLREVDAFPY
                     QLTLGALQNDSLIYKMGAAVASQCREAGINLNLAPVADVNNNPMNPVINYRSFGEDPE
                     KVAEKATMYMKGLQDNGIMACAKHFPGHGDTDTDSHTGLPVISGDRDRFDRIELLPFR
                     RLTDEGIGAVMTAHISVPGLDAGALPATFSKKVVTGLLRQESRFRGLILTDAMNMDGA
                     TMTFPSGIADAEALLAGNDIIEYPTDPVFAIEEIARRVGKGEIAVSEITDRCRRLLEA
                     KLWLESYTTANGGRAESGNQSIPGEAPAAAAGSSTPVSIPSSGHPALIRDLYAGAMTL
                     LENNDNLLPLGRLDRIRIATVSVNRLAMTEFQRMTDRYTNADHYFIDPANEQGARFVM
                     SKLMDYDVVIAGFCALDQKPAGLYGVTPALNNVFRQIASLDRAAVIWFGNPYGVSRLD
                     LTKKPTAMLVAYQDNSYTHQVAVQVLFGAIGASGRLPVTVNENYPAGTGIKTPGNIRM
                     QFGFPENAGLSSERLTGKVDSIVNAGLDTLAFPGCQVLIARRGIVVLNKCYGFHLYDS
                     TEAVTENDLWDLASVTKVSAATPSLMLLDDRGSFDIDKTLGNYLPWFRFSDKGDMVMR
                     EMLAHQAGLKSWIPFWRNTLENDSTFRRGIFSDYTNRKYALQVTDSMYMNRHYLKDMF
                     REIRDSPMGEKKYVYSDLTFILASEIIESLTDSTIDKFAPANIYNPIGAFNITYRPLE
                     KYPAERVVPTEYDSLFRKQLIRGTVHDEGAAMLGGVSGHAGLFATGNDLLKLVEMYRR
                     GGEYGGVRVLSSDVVRKYTAVQFPDNDNRRGLGFDKPLLRNDTLPPDEAYPCPSASPA
                     SFGHSGFTGTFIWCDPDEEISYVFLSNRVYPTRNNSRLSDLSIRGKILQAVYDARID"
     gene            3531..4658
                     /locus_tag="PLA17_06690"
     CDS             3531..4658
                     /locus_tag="PLA17_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPK85139.1"
                     /translation="MAATKSSGRRAGKSARLLSLDVFRGMTVAFMIIVNTPGTWSHIY
                     APLRHAPWHGCTPTDLVFPSFLFISGVAMFFSLRKYNFEFSGPSLFRILRRVLLIFAA
                     GLFLNIFPYFLRDYSTLRIMGVLQRIALAWGLGAILVLLIRRNYIWLATAVLLFGYWA
                     LMYFMGGADPYSLETNYARTVDIAVFGEKHLYKGFGIPFDPEGLLSTIPATATVLLGF
                     MSGGLISANRTSWKTPGWLALTGALLIGAGLLWGQYFPINKPIWTSSYVIYTGGIGMI
                     ILALLFIIIDIMNLKGWTGFFNTFGTNPLFTYLLAGIWTKTMLAVKIGESTLYKWIFE
                     HICSPLFEEQKLASLLFAIMQVLIIWAFGYILYRKKIIIRF"
     gene            4660..6078
                     /locus_tag="PLA17_06695"
     CDS             4660..6078
                     /locus_tag="PLA17_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPK85140.1"
                     /translation="MDIRTTVEKAFERWKGSPPEIITRLPQSGSDRVYFRLICREDQV
                     IGAYNPSREENEAFVGFSNHFRSKGLPVPEIYVYYPEDKIYFLEDLGDINLFTWLARR
                     SDEERFNKETEKLFHTIAGDLVRIQTEGIRGLDLSLCYPHRSFDRQSIVWDMNYYKYM
                     FLKLIGAPFNETRLERDFEILTRFLLDAGQEYFLFRDFQTANIMIRDGKPWYIDYQGG
                     RLGAPQYDIASLVYDAKAYIPENIRKTTIDRHLALFAEATGRSRESLEMYQGAFTLIR
                     LMQALGAFGFRGLHENKPTFNESIVPAVELMNELLESGDVKIDLPELKRASLAVPLQR
                     KYRILEHYQDMVTIHLESFAYVKGKIVNYDNGSGFVFDCRGLSNPVTVAELSELTGLD
                     PEVIEFFNNDDEAVAFAGDCTNIIRNSLPMMRRKGIVNIHAAFGCVGGHHRSVWSAER
                     VALMLSAMPGVEVSVKHTALEE"
     gene            6084..6803
                     /locus_tag="PLA17_06700"
     CDS             6084..6803
                     /locus_tag="PLA17_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPK85141.1"
                     /translation="MIFAAGLGTRLGEMTRTIPKALADINGRTMLELTAERVVRAGFD
                     DLIVNIHHHPEQMLEEIEKLRQQGYRITVSDESDELLDTAGGLYRARSFFGSEPFLAH
                     NVDEFTDLDLNGMYRQHVESEALATLAVRHRPGNRQLLVDAAGRLRGWRNNATKEEII
                     TVESSRQLEEVGFSGIQVLSPEIFDLMQEGIYSLTSLYLMLARKHPIMTCLHDYGYWF
                     DGGTPENLEKIRAHFGKQEVG"
     gene            complement(6833..8278)
                     /locus_tag="PLA17_06705"
     CDS             complement(6833..8278)
                     /locus_tag="PLA17_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPK85142.1"
                     /translation="MTSINIRSLLKLTGLIMIIAGGAFIACIPVALIYSESLGPFLLP
                     ALISFVPGLVLYMTMPRTSRDNTNIRDRYLNVTLSWVLLALLGTMPYLFSGSVSGFTN
                     ALFETMSGFTTTGATVIGNVEILPKSILFWRSLTHWIGGVGVIVLVIVILPNMKAGGY
                     HLFTLESSMKQKILPKTRSIAYIILIIYLAMTVLETVLLSFGEMSLFDSICHSFATVS
                     TAGFSTRNTSISEYSPYIQYVIGIFMFLGGVSYIVYYFLVRREFRKIAAFEELWLYIF
                     LVTGATILVTLILYTGTDRDLTLSFRHGFFQVISMMTGTGFATTDYMVWPQGGWLIML
                     LLMPAGGCTGSTTGGIKMARHLINLRNLKVVFQRFLHHHAVIPIKINNRIVPDNLNIT
                     ILSFTLLFIALTLAGMIAIHFTGVPLLEAVGASTSAISNVGPGLGMSGNFGHYNSFTS
                     GAKIIMTALMLIGRLEIFTILALFTKSFWKN"
     gene            complement(8304..9233)
                     /locus_tag="PLA17_06710"
     CDS             complement(8304..9233)
                     /locus_tag="PLA17_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPK85143.1"
                     /translation="MITYWQMIPNGLLPLNTFEKGCIVNVTDPTPSEIGILNTTLKVP
                     DDFLADILDVDERSRMEVEDDLLLMIYRIPYQNVSNGLPFTTIPLGLVLDKDNLVIIC
                     HRNNEVLDDFFSRSGKGITIETKIDLILHLFIRTTMFYHRYLKQINNQTTIIEQDLEK
                     STRNEELHRLLKMEKCLVYFTTSLRSNEAMMLKLKNSKWIRDYEFSSDLLEEATIENR
                     QAIEMAKIYSDIQSGMMDAFASVISNNLNIVMKSLTIVTIVLMIPTLIASFYGMNVPN
                     RLETNMSAFWFLLGGSVVLAAAAVILLRRKKWM"
     gene            9447..>10219
                     /locus_tag="PLA17_06715"
     CDS             9447..>10219
                     /locus_tag="PLA17_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPK85144.1"
                     /translation="MGKVLVVGAGGVGTVAVIKMAQMTDVFSEIMLASRTKSKCDTIA
                     DRVGGNRVQTARVDADNVAEMKELIRSFGPDMVVNLALPYQDLNIMEACLATGVAYLD
                     TANYEPPEEAKYEYSWQWAYHERFREAGITAILGCGFDPGVTSVFTAHAAKHHFSEIH
                     YLDIVDCNAGDHGKPFATNFNPEINIREVTQKGKYWENGEWVYTEPHEIHKTLTYPGI
                     GPKESYLIYHEELESLVKNFPTIKRARFWMTFSQEYLTHL"
BASE COUNT         2565 a         2621 c         2583 g         2450 t
ORIGIN      
        1 gacacgccag cttgaggggc tggtgctcgc aagggtgtgc aagttcgagt cttgtcctgg
       61 gcacacaaat aagaggccgt ctcaattcct gtgagatggc ctgttctttt cagccggtgc
      121 ctgtcctgtg agatggtctc ttcattttca gccggcgcct gtcctgtaag atgaccccct
      181 cttttcagcc ggtgcctggc actagcagct tgaaccgcca acgacgcaag gaagctcaat
      241 cctgcatccg ccggcagacg aatagcagga agagccggca tgaataaaga tggctgcagg
      301 ctatcgtgcc gtcaggcact aatacagcat caatcaatcg atccttgcat catacaccgc
      361 ctgcaaaatc tttcccctga tggagagatc cgaaagcctg ctgttgttcc gggtgggata
      421 aaccctgttg ctgaggaaca cgtatgaaat ctcctcatcc ggatcacacc agatgaaggt
      481 gccggtaaac cccgagtgcc cgaaactggc aggcgaggcc gacggacatg gataggcttc
      541 atcaggaggc aacgtatcat tacgcagcag cggcttgtca aatcccagcc cccggcgatt
      601 gtcattgtcc gggaactgaa cagctgtgta cttcctgacc acatcactgc tgagaactct
      661 cacacctccg tattcacctc ccctgcggta catctcaaca agcttcagaa ggtcattgcc
      721 ggtggcaaac aggcctgcat gacctgatac gcctcccagc atcgccgccc cctcatcatg
      781 aacagtgccc cttatcaact gcttcctgaa aagagagtca tactcggtag gaacaaccct
      841 ctctgccgga tacttctcaa gcggcctgta ggtgatgttg aaggcaccga tgggattata
      901 gatatttgcc ggagcaaact tgtctatggt actgtctgtg agactctcta tgatctctga
      961 agcaaggatg aaggtgaggt cagaatagac atattttttc tcacccatgg gactgtcacg
     1021 tatctctctg aacatatcct tcagataatg ccggttcatg tacatcgagt cagtaacctg
     1081 aagagcatac ttccggttgg tataatcact gaagatgcca cgcctgaatg tgctgtcatt
     1141 ctcaagggtg tttctccaga acggtatcca tgatttgagg ccggcctggt gtgcgagcat
     1201 ctcgcgcatg accatgtcac ccttgtcgga aaagcgaaac cacggaaggt aattgccaag
     1261 ggttttgtca atgtcaaacg aacccctgtc atcgagcagc atcagagagg gagtggccgc
     1321 tgataccttg gtcactgagg caagatccca caggtcattt tccgtgaccg cttcggtgct
     1381 gtcatataga tggaatccgt agcatttgtt aagcacgact attccccggc gggctatgag
     1441 cacctggcaa cccggaaaag ccagggtgtc aaggcctgca ttcactatag agtcaacttt
     1501 gcctgtcagc ctctccgagg agagtccggc attctccgga aacccgaact gcattcttat
     1561 gttgccgggg gttttgatgc ccgttccggc cggataattt tcatttaccg tcaccggcag
     1621 cctgccagag gcaccgatgg cgccgaagag cacctgcacg gctacctggt gagtgtaact
     1681 gttgtcctgg taggccacaa gcatggccgt cggcttcttc gtcaggtcaa gccgcgaaac
     1741 accatacgga ttgccgaacc atataacggc agccctgtcg agactggcta tctgccggaa
     1801 aacattgttc agggccggcg taacaccata aagccctgcg ggtttctgat cgagtgcaca
     1861 gaaaccggca atgaccacat cataatccat cagctttgac attacaaacc tggcaccctg
     1921 ctcattggcc gggtcaatga agtagtggtc agcattggtg tacctgtctg tcatacgctg
     1981 gaattcagtc atggccagcc ggttgacaga gacagtggct atccttatcc ggtcaagcct
     2041 gccaagtgga aggagattgt cattattctc cagcagtgtc attgctccgg catacaggtc
     2101 acgaatcagc gccggatgcc cggaagaagg gatgctgaca ggggtggaag agccggcagc
     2161 tgctgctggt gcctcgcccg gaattgactg attacctgat tctgctctgc ctccgttcgc
     2221 cgtcgtgtaa ctctcaagcc acagctttgc ttcaagaagc ctcctgcacc tgtcagtgat
     2281 ctcacttact gctatctcgc cctttcccac cctgcgggca atttcctcaa tggcaaaaac
     2341 agggtcagtg gggtattcaa ttatgtcatt tcctgccagc agagcctcgg catcggcaat
     2401 gcccgaaggg aaggtcatgg ttgcaccatc catattcatt gcatctgtaa ggatgagacc
     2461 ccggaaacgt gactcctgtc tgagcaaccc ggtcaccacc ttctttgaga aggttgccgg
     2521 aagagctccg gcatccaggc ccggcacact tatatgcgcc gtcatcaccg cacctattcc
     2581 ttcatctgtc agcctcctga agggtagcag ctctatcctg tcaaagcggt cgcggtcacc
     2641 acttattaca ggaaggccgg tatgggagtc agtgtcagtg tcaccgtgac ccgggaaatg
     2701 ctttgcacag gccatgatgc cgttatcctg aagccccttc atatacattg tcgccttttc
     2761 tgcaaccttc tccggatcct caccgaaaga gcggtagttt attaccgggt tcatcgggtt
     2821 gttgttcaca tctgctacgg gtgcaaggtt caggtttatc cctgcctcac ggcactgcga
     2881 tgcaacggca gctcccatct tgtaaatcag ggagtcattc tgcagtgccc ccagcgtcag
     2941 ctggtacgga aaggcatcca cctccctgag ccgcatccct gttccccact cagcatcctg
     3001 agcaatgatc agtggtatcc gggagatact cctgagccgt ttggtgtaat caacctgcat
     3061 ctcacgtgtg ccctcgaaaa aaatcacacc cccgatacca tacttctcaa ccagcagctc
     3121 tacctgcctg taattgcctc ccttttcatc tgcccatgcc ggcacccata tcagctgagc
     3181 tatcatttcc ctgatagtca tggtattgag tatgctgtca accccggggt ggccgatgaa
     3241 gcttttccat ggaacattat tgtctgtttc cctgaccgga gagtcagtct gcctggccgg
     3301 agaagcagtt tgtctgaccg gagagtcagc ctgcctggcc ggagaagcag tttgtctgac
     3361 cggagaggca gagattatca gggcgcaaaa aacaagtaca agtgaaaggg aggatttttt
     3421 catcagatga atgaattatg agttgaacca aaattagcat ttttctttat ctctcttttc
     3481 ccggttgatt agttccaaat tgttattttt acccaaccaa cagaatgcat atggcagcaa
     3541 caaaaagttc cggaaggaga gccggcaaat cagcgcggct tttgtcactt gacgttttca
     3601 gggggatgac tgtggctttc atgatcattg tcaacacccc gggcacatgg agtcatatat
     3661 atgctccgct gcgacatgcc ccgtggcatg gctgcacacc taccgacctt gtttttccct
     3721 cattcctgtt catctcgggt gtggcaatgt tcttttctct gcgaaagtat aacttcgagt
     3781 tctccggacc ttcactgttc cgcattctgc gaagagtact tcttatcttc gccgcaggcc
     3841 tttttctgaa tattttcccc tatttcttac gtgactacag cacgctccgg atcatgggag
     3901 tgctgcagag aatagccctg gcatggggtc tgggagcaat tctggtgctg ctgatccgca
     3961 ggaattacat ctggctggcc acggcggtgt tgctgttcgg atactgggcc ctgatgtatt
     4021 tcatgggagg ggccgatcca tattcactag agaccaacta tgcacgaacg gttgatatcg
     4081 ccgtgttcgg cgaaaagcac ctctacaagg gatttggcat accatttgac ccggaaggac
     4141 tgctgagcac cattcccgcc accgcaacgg tactgctcgg gtttatgtca ggaggcctca
     4201 tcagcgctaa cagaacatca tggaaaacac cgggatggct tgccctgaca ggcgccctcc
     4261 ttatcggggc aggattgctg tggggacagt atttcccgat caacaaaccc atctggacaa
     4321 gctcctatgt catctacacc ggcggtattg gtatgataat cctggccctg ctcttcataa
     4381 taattgatat catgaacctg aaaggatgga caggcttctt caatacattc ggcactaatc
     4441 ccctcttcac atacctcctc gccggaatct ggaccaagac aatgcttgcc gtgaagatag
     4501 gggagtccac actttacaaa tggatattcg aacatatctg cagtccgctc tttgaggaac
     4561 agaagcttgc aagcctgctc ttcgcaataa tgcaggttct gataatctgg gcgttcggat
     4621 acatacttta caggaaaaag ataattatca ggttctgaaa tggatataag aacaacagta
     4681 gaaaaggcat ttgaacgctg gaaaggctcc cctccggaga ttatcacccg cctcccgcag
     4741 tcgggctcag acagggttta tttcaggctg atatgccgtg aagatcaggt gataggcgca
     4801 tacaatccca gccgcgagga aaacgaagct ttcgtcggtt tttcaaacca cttcagatca
     4861 aagggattgc cggtgccgga gatatatgtt tattatccag aagataagat ctattttctt
     4921 gaggatctgg gcgatatcaa cctttttaca tggctggccc gccgttccga tgaggagcgt
     4981 ttcaacaagg agacggaaaa gctctttcac accatcgccg gtgacctggt acgaatacag
     5041 actgaaggaa tcagggggct tgacctgtca ctctgttatc cccaccgcag cttcgaccgg
     5101 cagagcatag tctgggacat gaattattac aagtacatgt ttctcaaact gataggagcg
     5161 ccgttcaatg agaccaggct tgaacgggat tttgagattc tgacccgttt cctgctggat
     5221 gcaggacagg agtattttct cttccgcgac ttccagacgg caaatatcat gatcagggac
     5281 ggaaagccgt ggtatattga ctaccagggc ggcaggctcg gggcaccgca gtatgacatc
     5341 gcttcactcg tttatgacgc aaaggcctat atccctgaaa atatcaggaa gacaacgatc
     5401 gacaggcacc tggctctctt cgcggaagct acaggcagat cacgtgagtc acttgagatg
     5461 taccagggag cctttaccct gataaggctc atgcaggcac tgggcgcctt cggattcagg
     5521 ggactgcatg agaacaaacc tactttcaat gagagcatcg tgccggccgt ggagctgatg
     5581 aatgagcttc tcgagagcgg tgacgtaaag attgatctcc ccgaactgaa gagagcctca
     5641 ctcgcagtgc cgctgcaacg aaagtaccgc atcctggaac actaccagga tatggttaca
     5701 atacatctgg agagctttgc ttatgtaaag gggaaaattg taaactatga caacggcagc
     5761 gggtttgtct ttgactgccg cggactgagc aatccggtca ccgtggctga attgagcgaa
     5821 ctcaccggcc ttgatcccga ggtgatagag ttcttcaaca acgatgatga agcagtggct
     5881 tttgcaggcg actgtacaaa catcatccgc aactcgcttc ccatgatgcg acggaagggt
     5941 atagtcaaca tacatgctgc attcggctgt gtcggcggcc atcatcgctc cgtctggagc
     6001 gcggaaagag tggctctgat gctctcggca atgcccggtg ttgaagtgtc agtgaaacat
     6061 accgccctgg aagaatgaag gcaatgatct ttgcagccgg ccttggcacc aggctggggg
     6121 agatgacccg taccataccc aaggcgctgg ctgacatcaa tggcaggacc atgctggaac
     6181 ttacggcgga gagagttgtc agggcaggat ttgatgacct gattgtcaat atccaccacc
     6241 atcccgaaca gatgcttgag gagatagaaa aactcaggca gcagggttac cggattaccg
     6301 tcagcgacga aagtgatgag ttgctggata ctgcaggcgg tctttacagg gcaaggagct
     6361 tctttggctc cgaaccattc cttgcacata acgttgatga attcactgat cttgacctca
     6421 atggcatgta caggcaacat gtggaatcag aagcactggc caccctcgcg gtgagacacc
     6481 ggccggggaa ccggcaacta cttgtagatg ctgcagggcg ccttcgcgga tggcgcaata
     6541 acgcaacaaa ggaagagatc attaccgttg agagcagcag gcagcttgaa gaggttggct
     6601 tttcaggcat tcaggtcctc tctcctgaaa tctttgatct gatgcaggag ggtatctatt
     6661 cgctgacatc gctctacctt atgctggcac gaaagcatcc aatcatgacc tgcctgcatg
     6721 actacggtta ctggttcgac ggcggcacac cggagaacct cgaaaagatc agagcacatt
     6781 ttgggaagca ggaagtgggc tgacagggaa agcaatcagc ctccggaaag gttcagtttt
     6841 tccagaatga ctttgtgaag agggccagta tggtaaaaat ctcaagacgc ccgatgagca
     6901 tcagtgcagt cataataatc tttgctcctg acgtgaaact gttgtagtgg ccaaagttgc
     6961 ccgacatgcc aagccccggc cccacattgc ttattgcgct ggtacttgct cctacagcct
     7021 caagcagggg caccccggtg aaatggatgg caatcatccc ggccagggtc agggcaatga
     7081 acagaagggt gaatgaaaga attgtaatat tcagattgtc aggcactatc ctgttgttta
     7141 tcttaattgg tatcaccgca tggtgatgca ggaaccgctg aaagacaacc ttcaggttac
     7201 gcaggttgat caggtgccgg gccatcttga ttcctcccgt ggttgatcct gtacatcctc
     7261 cggcaggcat cagaagaagc atgattaacc atccgccctg tggccagacc atgtaatccg
     7321 tcgtggcaaa gccggttcct gtcatcattg agataacctg gaaaaagcca tgcctgaagg
     7381 agagggtaag atcgcggtca gtgccggtat agaggatcag agtcacaagg atggtggccc
     7441 cggtgacaag gaatatgtaa agccatagct cctcaaaagc agctatcttc ctgaactccc
     7501 ggcgcacaag aaaatagtac acaatataac tgacccctcc gaggaacata aagattccta
     7561 tcacatactg gatatatggt gagtattccg agatgctggt gttcctggtg gagaaacctg
     7621 cggtggatac tgtggcaaag gagtgacata tgctgtcaaa aaggctcatc tccccaaaac
     7681 taagaagcac agtctccagt acagtcatcg ccaggtagat gatcagtatt atgtatgcta
     7741 tagaccttgt cttgggcagt atcttctgtt tcatcgatga ctcgagggtg aagagatgat
     7801 accctccggc cttcatgttg ggaagaatca ctattacaag tacgatcacg cccacaccac
     7861 ctatccagtg agtcaggctc cgccagaaga ggattgactt cggcagaatc tcaacattgc
     7921 ctattacggt cgccccggtt gtggtaaaac ctgacatggt ttcaaacagc gcattggtga
     7981 accctgacac tgacccgctg aagaggtagg gcatggtgcc aagcagggcc agcaaaaccc
     8041 atgaaagagt cacattcaga tacctgtccc tgatattggt attatccctt gatgtgcggg
     8101 gcatggtcat gtagagcaca agccccggca caaatgaaat aagtgccggc aagagaaacg
     8161 ggccgaggct ctcggaatag ataagtgcaa caggaataca ggcaatgaaa gcaccacctg
     8221 ctatgatcat tatcaggccg gtaagcttca gaagtgatct gatgttgatg ctggtcatta
     8281 cagagcggcg cgaccgtact cagctacatc catttctttc ttctcaggag aattacggcg
     8341 gcagcggcaa gcaccaccga accgcccagc agaaaccaga atgcactcat gttggtctcc
     8401 agcctgttgg gtacgttcat cccgtaaaag ctggcaatga gggttggtat catcaatacg
     8461 atggtgacta tcgtgagaga tttcatcacg atattcagat tgtttgagat gaccgaggca
     8521 aatgcatcca tcatcccgct ctgaatatcg ctgtaaatct tggccatctc aattgcctgc
     8581 cggttctcta tggtagcttc ttccaggagg tctgacgaaa actcgtagtc acgtatccat
     8641 ttcgaattct tcagcttgag catcatcgcc tcatttgatc gcagcgaggt ggtgaaatag
     8701 acaagacact tctccatctt cagcagtcgg tgcagctcct cgttccgggt cgacttctcc
     8761 agatcctgct caattattgt cgtctggttg ttgatctgct tcagatacct gtggtagaac
     8821 atcgtggttc tgataaagag atgcagtatg agatcgatct ttgtctctat tgtaataccc
     8881 ttgccgctgc ggctgaagaa atcatcaaga acctcgttgt tccggtgaca gatgattacc
     8941 aggttgtcct tgtcaagcac gaggcccagg ggtatggttg tgaatggtaa tccgttcgac
     9001 acgttctgat atgggatacg gtagatcatc agcagcaaat catcctccac ctccatgcgc
     9061 gatcgctcgt caacgtcaag tatatcagca agaaaatcat ccggaacctt cagtgtggtg
     9121 ttcagtatgc caatctctga aggggtggga tcagtgacgt ttacaatgca acctttttca
     9181 aatgtattta acggaaggag accgttggga atcatttgcc agtaagtaat cattttagta
     9241 cctcctactt ttctgctccg cggaggcacc gttaccggct tacccctgca gaaccaaatt
     9301 aataaaacgg ctcgcgtgat aatcgtccat aaaagacaac tgtattgata cagtgtgcaa
     9361 aaatataact tttctgatac ccgcatcccg ggtccgtgaa aaatgtacaa aaaaattacc
     9421 tttgcacaga acctaaatat aacgtaatgg gaaaagtact tgttgtgggc gctggtggtg
     9481 ttggcaccgt tgcagtgatt aaaatggcgc agatgactga cgtttttagt gagataatgc
     9541 tggccagccg cacaaaatca aagtgtgata caattgcaga cagggttgga ggcaacaggg
     9601 tgcagacagc acgggtagat gctgacaacg tggctgagat gaaggagctt atccgctctt
     9661 tcgggcctga catggtagtc aatctcgcac ttccctacca ggatcttaac ataatggaag
     9721 catgccttgc cactggcgtt gcctaccttg atactgccaa ttatgagccg cctgaggagg
     9781 caaaatatga gtactcatgg cagtgggcat atcatgagcg cttccgcgaa gccggcatca
     9841 cggcgatact tggctgcggc tttgacccgg gtgtcaccag tgtctttaca gcacatgccg
     9901 cgaaacatca cttcagcgag atacactatc tcgatatagt tgactgcaac gccggcgatc
     9961 atggcaaacc ctttgccaca aacttcaatc ccgaaataaa catcagggag gtaacccaga
    10021 aaggcaaata ctgggagaac ggagagtggg tttacactga accccatgag attcacaaga
    10081 cactgacata ccccggcatt gggccgaaag aatcatacct gatctaccat gaggaactgg
    10141 aatcgctcgt gaagaacttc ccgacgataa agagggcccg tttctggatg actttcagcc
    10201 aggagtatct tactcacct
//
LOCUS       DAOPBK010000093         9981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6495_length_9981_cov_4.504937, whole genome shotgun sequence.
ACCESSION   DAOPBK010000093 DAOPBK010000000
VERSION     DAOPBK010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6495_length_9981_cov_4.504937"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLA17_06720"
     CDS             complement(<1..321)
                     /locus_tag="PLA17_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85145.1"
                     /translation="MKKILLPLFYTLLAAGFLLVLLLWIIVLRENVKNDEPVRIYLPT
                     GSDFSALTDTLESADLLRSRESFIITSRLKSFGKTVKPGSYIIEPGMSNNSLVNMLRS
                     GRQAP"
     gene            complement(398..1879)
                     /gene="cls"
                     /locus_tag="PLA17_06725"
     CDS             complement(398..1879)
                     /gene="cls"
                     /locus_tag="PLA17_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPK85146.1"
                     /translation="MNETKMILAGNTIYIIKNLLTVIYLIAVFVICLMIVFENRNPLK
                     TLSWLLVILFVPVIGVILYFFFGRNYRKQKIYTRKSMADSARLIGHADRQARLLSSVL
                     AGESDAIRSKSHLMHLMLKNNRSLLTLDNKIDLLVNGSQAFPSMLDALASATSFIHLE
                     FYRIEPDNIGMEFSELMMRKAREGVKVRVIYDDVGSWNIRKPYINMMREAGVQIYSFL
                     PVRFPSFSSKINYRNHRKILVVDGKVGFVGGLNIADKYLHGLPDLGPWVDTHLRLEGE
                     AVAALDRVFLADWDFVSGEELPPDGSLEHVVHTGNRCLVQVASSGPDTDWATIMQVYF
                     SAIATAKSSIYLTSPYFSPDESLLTALKTSALSGVDVRMIFPEYSDSVIANWNTRSYI
                     MELLEAGVRIFLYRNGFIHSKYLLVDSIFSSVGSPNVDVRSFDLDFEVTALIYDEDFA
                     LRLGVLFAEDLKNCVEVTMQEWVKRKRRERYRESLARIFGPLY"
     gene            complement(1951..2568)
                     /locus_tag="PLA17_06730"
     CDS             complement(1951..2568)
                     /locus_tag="PLA17_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPK85147.1"
                     /translation="MKKFIVIILALVLAAPLFSQVKFGLKAGASTDFTFTDQTIKGTD
                     CEVILQNAKDAEWGLQGGVFMRATFSGFYIQPELLFATATNSVSYEDVEAGGTAVIYN
                     QKFNKLNIPLLLGVKIGPLRLNAGPAASVMISDPKEVIQGATYKRATFGYQAGLGFDI
                     LNKLTLDLRYEGNLNQFGDEIKIGSHTYQLDDRTGALLLHVGLIF"
     gene            complement(2753..2866)
                     /locus_tag="PLA17_06735"
                     /pseudo
     CDS             complement(2753..2866)
                     /locus_tag="PLA17_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(3189..3593)
                     /locus_tag="PLA17_06740"
     CDS             complement(3189..3593)
                     /locus_tag="PLA17_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85148.1"
                     /translation="MERLCLDCGARLLGRSDKKFCSDQCRNNYNNRLNRDQNNYVRNV
                     HAQLRRNRRILSDLFGDGHHHIHRDALIAQGYNFTFFTHLVETSDGLRWSFCFEYGFR
                     VSGKGYLELQKSSSYLDLVPEEQEDQDNQDDQ"
     gene            3798..4262
                     /gene="ssb"
                     /locus_tag="PLA17_06745"
     CDS             3798..4262
                     /gene="ssb"
                     /locus_tag="PLA17_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPK85149.1"
                     /translation="MNALRNKVQLIGRLGQDPEFRTLEGGKKVARFNLATNESYKNSE
                     GARIDETTWHSIVVWNGLAELTSKYLTKGKEVCIEGRISYRSYTDKNGIQRNVTEIVA
                     SDMVMLGSGSRPRNMINDEPVVDVAGDPSAGMADEPAEAGASTRRSGRAREK"
     gene            complement(4362..4940)
                     /locus_tag="PLA17_06750"
     CDS             complement(4362..4940)
                     /locus_tag="PLA17_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPK85150.1"
                     /translation="MKCDIILAGVGGQGILSIAATIGLAAVEQNLFLKQAEVHGMSQR
                     GGDVQSHLRLSDSEIFSDLIPHGGADLIISVEPMEALRYLPWLSENGWLVTNSVPFVN
                     ISNYPEAGEVLAELDKAGNTIVLDADSIARDLGSTRSGNMVILGAASSFISMPFSALE
                     DAVRKMFSRKGEEVVEVNLKALRAGRDAAAAR"
     gene            complement(4942..6561)
                     /locus_tag="PLA17_06755"
     CDS             complement(4942..6561)
                     /locus_tag="PLA17_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPK85151.1"
                     /translation="MHKLLLLGDEAIAQAALDAGLSGMYAYPGTPSTEIMEYIQASPQ
                     AAADGVHREWSSNEKTAMEAALGMSYAGKRAMVAMKHVGLNVAADAFINSAVTGVNGG
                     LVVVSADDPSMHSSQNEQDSRFYGRFAMVPVMEPRNQQEAYDMIFEAFALSERYRLPV
                     LFRITTRLAHSRSSVARRDRIAQSSVSMPSDPRRFILLPAIARRQYRALAEAYEKLYT
                     DDAVDGLNRLKTGNGRGTGVIACGIAYNYLMENIADRAGDYHVLSVGAYPLNREKVAA
                     LVDNCDRVLVLEEGYPIIEEQLRGLLDRDVKVLGRMDGTVPRDGELNPSIVATALGLK
                     AGQGREVPPLVRQRPPSFCKGCGHADMYQALTEVMAEAGQGRVFADIGCYTLGALPPY
                     NIVNSCVDMGASVTMAIGAADAGLFPAVAVIGDSTFTHSGMTGLLDAVIKNSPITVII
                     SDNYTTGMTGGQKSQATDRLENICLGLGVDPEHLKVIVPLRKNHAENKVVIRNELNYH
                     GISVILARRECIQTASRSKKARAAEKDKSKE"
     gene            6679..7359
                     /locus_tag="PLA17_06760"
     CDS             6679..7359
                     /locus_tag="PLA17_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85152.1"
                     /translation="MKKLFVICIMLLFSFPRLGAQGGERLYIQIDGMTADESGFIVRD
                     VSIYSTHLRRGAASDHRGIFSIISTPGDTVFFSCIGYKPTLLTIPVTATGDHYTTDVM
                     MVTDTIQIGEVLVLPWKTYSEFKRAVLENKPKDPLMENLEYNLAMVQQQIWSDTRPTP
                     GEAYRYTMQQMSDELYTRGQRPGGNLLNPFAWQKFVEGVKSGMLKNEPSKEVRVKKQK
                     SRKKKRRD"
     gene            7359..9782
                     /locus_tag="PLA17_06765"
     CDS             7359..9782
                     /locus_tag="PLA17_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPK85153.1"
                     /translation="MVRSSAIRVLTASILLSVISAGFLNGQMKVISGIVTDTTGVGVA
                     GATVFIPSVERGTLCGNDGSFRLAFTPGESGEFMVIFSCVGYNTDTVTLKASGALDGI
                     RVALIPATTIIDGVEVRARRPDNITVISIPAVAGAVIPSVSGGVEAAVATLPGVSTFS
                     ELSSGYSVRGGSYDENLVYLNGVEVYRPYLLRTGQQEGLSRINPDLTASVNFSPGGFS
                     AAYGDRMASVLDITYREPEYREGSVSLSLLTSSVHAGAHSDDNRFWFIAGARYRSNAI
                     LLGGLDTRGAYRPHFADIQAIGGFRPDGRTGITLTLWGSMNRYTFLPRSQTTTFGTFE
                     NAYRLYAFFEGGERDRYYSGGGALTLERKHTDRHSSKLIISTNLADEYENHDIRGAYS
                     LSALDRNLGSENNPDTLLNAGAGSWLSHARNSFRSLISGVSYRGSLSSGRNGLEWGLA
                     VRHRSYDSFRNEWLRVDSAGYTQGQQGDWLALTTRTLLGNETRSFTSEGWLISRRHFT
                     VAGRACDLNAGIRVSYDTYCSEMLVSPRVSLRSKITRQVSLYCAAGAYHQPPEVRELI
                     SEDGEGGSDPKAQRSYHLTAGVHYDFMAWDRPFRFTAEAYGKLFDRLIPYKTDNVRLI
                     YYGGNIAEGYSAGIDMRVNGEFVPGVESWFSMSLMKSEMQIPGLSTGWYPAPFDQRIH
                     FSIFFQDYLPGHPDFRAHINVAFGTGIPTSPPDHEQWDIWFRMPPYRRIDIGFSKVLL
                     GAGRANRDSFMKELVAGIEIFNLSDIRNTISYTWIRTVRNSQGETREYAVPNYLTRRS
                     LNLKLTAHF"
BASE COUNT         2579 a         2567 c         2379 g         2456 t
ORIGIN      
        1 cggggcctgt cggcccgagc gcagcatgtt gacaagactg ttgttgctca ttccgggctc
       61 aatgatatat gaaccaggtt tgactgtttt tccgaaagac ttcagccggg aggtgattat
      121 gaagctttcc ctgctccgca ggagatcagc actctccagc gtatcagtca gagcactgaa
      181 atctgacccc gtgggaagat atattctaac cggctcatca ttcttcacat tctccctgag
      241 cactataatc cacaaaagca gtaccagcag aaatccggca gcaaggagag tgtaaaaaag
      301 tggcagaagt atctttttca tcaggtgcta atttaattga gagtacagaa tattgaaagt
      361 tgttttacct gtcagaaggt tgatatttgg tgtgaattca atagagagga ccgaatatcc
      421 gtgccagtga ctccctgtat ctctctctcc tttttcgttt tacccactcc tgcatggtta
      481 cctctacaca gttcttaagg tcctcggcaa agagcacccc aagacgcagc gcaaaatcct
      541 catcataaat cagggcagta acctcgaagt caaggtcaaa actgcggaca tcaacgttgg
      601 gagaccctac tgaggaaaag atactgtcaa ccagcaggta tttggaatgt atgaatccgt
      661 tacgatacag gaagatcctc acacctgcct cgagcagttc catgatatag gaacgggtat
      721 tccagttcgc aataacagaa tctgagtatt caggaaaaat cattctcaca tcaacgccgc
      781 tcagggcaga ggtcttcagt gctgtcagca ggctctcgtc agggctgaag tagggtgatg
      841 tcagatatat tgacgatttt gccgtagcga ttgccgaaaa gtaaacctgc attatcgtgg
      901 cccagtcagt gtcaggtccg ctggaggcaa cctgaaccag gcacctgtta ccggtatgga
      961 cgacatgctc gagtgaaccg tcaggtggca gttcttcacc actcacaaaa tcccagtcgg
     1021 ccaggaaaac cctgtcaaga gccgcaacgg cttcaccctc aagcctgaga tgggtatcaa
     1081 cccaggggcc cagatccggc agtccgtgca ggtacttgtc tgctatattc aatccgccta
     1141 caaagccaac ctttccgtca accaccagta ttttcctgtg attccggtag ttgatcttgc
     1201 ttgagaaact gggaaaacga accggaagga acgaatatat ctgcacgccg gcttcacgca
     1261 tcatatttat atacggtttg cggatgttcc agcttccgac atcgtcatag atgaccctga
     1321 cctttacccc ttcccttgcc ttgcgcatca tcagctcact gaattccatc ccgatgttat
     1381 ccggttcaat gcggtagaac tcaagatgaa taaagctggt ggccgaagcc agggcatcaa
     1441 gcattgaagg aaacgcctgg ctgccgttta ccaggaggtc tatcttgttg tcaagggtaa
     1501 gcagagatct gttgttcttg agcatcaggt gcatgagatg actctttgac ctgatggcat
     1561 cactctcgcc ggccagtacc gatgaaagca gcctggcctg gcggtcagca tgaccaatca
     1621 gtctggcgct gtcagccatg ctcttccggg tataaatctt ttgtttgcgg taattcctgc
     1681 cgaaaaagaa atagaggatg actccaatca cgggaacaaa caggatcacc agcagccatg
     1741 aaagggtctt gagaggatta cggttctcaa agacgatcat cagacagatg acaaatacag
     1801 ctatcagata gataactgta aggaggtttt taatgatata gatggtattt cctgccagga
     1861 tcatcttggt ttcgttcaca tactaccgta aatataaaaa aagaactctt cctgcaaact
     1921 gcggaagagc tcttttgtat gtcacgaata tcagaatatc agtcccacgt gcagaagcaa
     1981 tgctcctgta cggtcatcaa gctggtaggt atggcttccg atttttatct cgtcaccaaa
     2041 ttggttgagg ttcccttcat acctcaggtc cagggtgagc ttatttaaaa tatcaaatcc
     2101 aaggcctgcc tggtatccga aggttgccct cttgtaggtt gctccctgta tcacctcctt
     2161 cgggtcagat atcataactg aagctgcagg tccggcattc agtctcagcg gtccaatttt
     2221 taccccgaga agaagtggaa tattcagttt attgaatttc tggttgtaga tcactgcagt
     2281 gccgccggct tcaacatcct catacgatac tgagttggta gctgtggcaa aaagcagttc
     2341 gggctgaata tagaatcctg aaaaggttgc tctcatgaat actcccccct ggagtcccca
     2401 ttcagcatcc ttcgcatttt gtagaatcac ctcgcaatcg gtacctttaa tggtctgatc
     2461 ggtaaaggtg aagtcagtgc ttgccccggc cttgagaccg aattttacct gtgaaaacaa
     2521 cggagcggca agaaccagtg caagaataat aacaataaac tttttcatct ctatcagttt
     2581 tggttaattt gacaatagtt gttgttctga taaacgcaag attcaggcca aaggttgcac
     2641 aggatgccgg aaaagagtct gtttttttac ttttttgcca gctccgagaa tataactgac
     2701 ttcattttat caaactcttc acgctcaaaa aggcgggtaa gaaagattat cctgccgtaa
     2761 actgaagcat caccaccttc cccttcaggt cagacagctt atagctcttc ccgccaactt
     2821 ctttaaccgt aaaatcaggc gcactgtcac ccacttttac aagatagttg tgctcatata
     2881 ctttctgccc gtgcatcgcc ggtaaaacca gcagcatcat gacaataacc atagaaattt
     2941 tccttgccat atgaatatat tttaagtttt agagaatcag cagtatggat ggaatatgaa
     3001 aaagtaatat ttcatgctcg taatcaaaag gcaataattg cgggtttcac ccctggttgt
     3061 gattgttgga tcatgtgggg attcatagta tccttcatca ggagttcaaa agtaacattt
     3121 tcacatgttc ccgagcctga tcccgatggt gttttccggc acttttttta ttcagacctg
     3181 tctcatgttc attgatcatc ctgattatcc tgatcttcct gctcttccgg aaccaggtca
     3241 agatagctgc tgctcttctg aagctccaga taacccttgc cagagaccct gaagccatat
     3301 tcaaaacaga aagaccagcg taaaccatca cttgtttcga caagatgtgt aaagaaggta
     3361 aaattgtagc cctgggctat aagtgcatca cggtgaatat ggtgatgacc gtccccgaaa
     3421 aggtcagaaa ggattctcct gttacgcctg agttgcgcat gaacattgcg cacatagttg
     3481 ttctgatcac ggttaagacg attgttgtag ttgttccggc actggtcaga acagaatttc
     3541 ttatctgacc gtccgaggag cctggctccg cagtcgagac ataacctttc cacagcaaga
     3601 tatttttata aagataaaaa aaatcatgga acaggcataa ggaaaaccgt atatcattct
     3661 gattaatatt cagtaacccg tttacaaacg gatataaacg actgctaaac gtatagaagt
     3721 ggttaacaac cgaatcaatt tccggcgagg gtgcacattt gtatcgacaa cagcattatt
     3781 aaccaaaaac gaatgtcatg aatgcattaa gaaacaaagt tcagctgatc ggtcgcctgg
     3841 gacaggaccc ggaattcaga acgcttgagg gcggaaagaa ggttgcccgc ttcaacctcg
     3901 caaccaatga gagctacaaa aactcggaag gtgcaaggat cgacgagacc acctggcaca
     3961 gtatcgtggt atggaacggt cttgccgaac tcacctcgaa gtatctcaca aagggtaagg
     4021 aagtatgcat cgaggggcgc ataagctatc gctcctacac tgacaaaaac ggaatccaga
     4081 ggaacgttac ggaaatcgtg gcctcagaca tggtaatgct tggctccgga tccagaccga
     4141 gaaacatgat caatgatgaa cccgtggtgg atgttgccgg tgatccgtca gcagggatgg
     4201 ctgatgaacc ggctgaggcc ggtgcttcca cccgccggag tggcagagcc agggagaagt
     4261 gaatcaggca cttcgctgag ggtgtctcgc tggcaggctc cggcatagcg ccggggcctg
     4321 tttgctgcgc cgcctttcct ctcctcaggc agtcagacct gttacctggc cgctgccgca
     4381 tccctcccgg cacgcagcgc cttcagattg acctcaacga cctcttctcc cttgcggctg
     4441 aacatcttcc tgaccgcatc ctcaagcgca ctgaagggca tcgatatgaa tgacgaagca
     4501 gcgcccagta tgaccatgtt tcccgaccgg gttgacccca ggtcacgtgc aatgctgtct
     4561 gcatcaagca caatggtgtt tcccgccttg tccagttcag ccagcacctc tcccgcctcc
     4621 gggtaattgg agatattcac aaaggggact gaattggtta ccagccatcc gttctctgag
     4681 agccatggca ggtagcgaag tgcctccata ggctccacac ttattatcag atctgcacct
     4741 ccgtgcggta tgagatctga aaagatctca ctgtcagaga gacgcaggtg cgactgcacg
     4801 tcacccccgc gttgactcat gccatgaacc tctgcctgtt taagaaagag attctgttca
     4861 acagctgcaa gccctatcgt ggctgcaatt gagagaatac cttgtcctcc cactcctgct
     4921 agtataatat cacatttcat atcattcttt tgatttgtct ttctcagccg ccctggcttt
     4981 tttgctacgt gatgccgtct gaatgcactc ccgtcttgcc agtatcaccg agataccatg
     5041 ataattgagt tcattgcgga taacgacttt gttctctgca tgattctttc tcagaggcac
     5101 tatcaccttc agatgttcag gatcaacacc cagtcccaga cagatgttct ccagtctgtc
     5161 agtggcctgt gatttctgcc ccccggtcat cccggtggtg tagttgtcag aaataatcac
     5221 tgttatcggt gagttcttga tcaccgcatc aagcagtcct gtcatgcctg aatgggtgaa
     5281 ggtagagtca cctatcactg ccactgccgg aaacaggccg gcatctgcag ctccgatggc
     5341 cattgtcacc gaggctccca tatcaacaca tgaattgact atgttgtacg gtggcagggc
     5401 gccgagggta taacagccga tatctgcaaa aacgcgtccc tgtccggctt cagccatcac
     5461 ctccgtaagc gcctggtaca tgtcggcatg tccgcacccc ttgcaaaacg acgggggacg
     5521 ctgacgcacc agcgggggga cctccctgcc ctgaccggct ttgagaccca aagcagtggc
     5581 aacaatcgaa ggattcagct ccccgtcacg gggtaccgtg ccatccatcc tgcccagaac
     5641 tttaacgtca cgatcaagaa gaccccttag ttgctcctct attatcggat aaccctcctc
     5701 cagcacaaga accctgtcgc aattatccac cagcgctgca accttttccc tgttgagcgg
     5761 ataagcaccc actgaaagaa cgtgataatc acctgccctg tcagcaatgt tctccatcag
     5821 gtaattgtaa gcaatcccgc aggcaatgac cccggtgccc ctgccgttgc cggttttcag
     5881 cctgttaaga ccgtcaacag catcgtcagt gtacagtttc tcatatgcct ctgcaagagc
     5941 cctgtactgc ctcctggcta tcgcgggaag aagtataaac cgacgaggat ctgaaggcat
     6001 tgaaacactg ctctgagcaa tcctatccct ccgtgcaaca gatgatcttg aatgtgccag
     6061 cctggttgtg atcctgaaca acacaggaag cctgtacctc tctgacaggg caaaggcttc
     6121 aaagatcatg tcataggcct cctgctggtt acgcggttcc attaccggca ccatggcaaa
     6181 cctgccataa aagcgtgagt cctgctcatt ctgggatgaa tgcattgagg gatcatcagc
     6241 agaaacaacc accagccctc cgttgactcc cgtcaccgcc gagttgatga atgcgtctgc
     6301 tgcaacattc aaacccacat gcttcattgc aaccattgcc ctcttgccgg catatgacat
     6361 gcccagggca gcctccatgg ctgtcttctc atttgatgac cattcacggt gaacaccgtc
     6421 tgccgcagcc tgcggcgaag cctggatgta ctccataatc tcagttgacg gagtaccagg
     6481 ataagcatac attccgctca gtcccgcatc cagagcagcc tgcgcaatgg cctcatctcc
     6541 aagcaataag agtttatgca taattattta gctttagtta gttcacaatt acggtgacaa
     6601 taaagatgat taaagttatg ttaaaatcaa gtagcaaatg tcattattgt ttactttacg
     6661 tcataaaatt atcagtcttt gaagaagctt tttgtgattt gtatcatgct gttgttctct
     6721 ttcccgcggc tgggtgccca gggcggtgag agactatata tacagataga cggcatgaca
     6781 gcagatgaaa gtggattcat tgttcgtgat gtaagtatat attcaacgca cctgcgccgg
     6841 ggagctgcaa gtgatcacag gggtatcttc tctataataa gcactcccgg tgatactgta
     6901 ttcttcagtt gcataggcta taaaccgaca ctgctgacca tccccgtaac tgccacaggc
     6961 gatcattaca caacagatgt gatgatggtc accgatacaa tccagatcgg agaggtactt
     7021 gttcttccct ggaagaccta ttcggaattc aagagggctg ttctcgaaaa taaacccaag
     7081 gaccccctga tggaaaacct ggaatataac cttgcaatgg tccagcagca gatatggagt
     7141 gacacgcgtc ccacacccgg agaagcatac cgctacacca tgcagcagat gtctgacgag
     7201 ctctacacca ggggccagag gcccggcggc aacctgctca atccatttgc ctggcagaaa
     7261 tttgtcgagg gagtaaaaag cggaatgttg aagaatgaac cctcaaagga ggtcagggtt
     7321 aagaagcaaa agagccgcaa gaaaaaaagg agagactgat ggtcaggtct tcagccataa
     7381 gagtgcttac agcttccata cttctgtcag ttatctccgc cggcttcctc aacggacaga
     7441 tgaaagtcat ctccggtatt gtcacagata ccaccggggt gggggtggca ggtgctacgg
     7501 tgttcattcc ttccgttgaa aggggaacat tgtgcggaaa tgacggttca ttcaggcttg
     7561 ccttcacccc cggtgaatcc ggggagttta tggtcatctt ctcatgtgtc ggatacaata
     7621 ctgacactgt caccctgaaa gcaagcggag ctcttgatgg tatccgtgtg gctctcatcc
     7681 ctgcaacgac aatcatcgat ggtgtagagg taagagccag gcgccctgac aacatcaccg
     7741 tgataagcat accagcggta gcaggtgcag ttataccatc ggtaagcgga ggtgtggagg
     7801 cagcggttgc cacactcccg ggagtcagca ctttcagtga gttgagctca ggctattcag
     7861 tcaggggtgg cagttacgac gagaacctcg tctaccttaa cggcgttgag gtttaccgac
     7921 cgtatctcct ccgtacaggc cagcaggaag ggctcagcag gatcaatccc gatctgaccg
     7981 cctcagtaaa cttttccccg ggaggattca gcgctgccta cggtgaccgt atggcatcag
     8041 tgcttgacat aacttaccgg gagcccgaat accgtgaggg atcggtctcg ctgagcctgc
     8101 tcacctcatc ggtacatgcc ggggctcaca gtgatgataa caggttctgg ttcatcgccg
     8161 gagcccgcta ccggtcaaat gccatcctgc tgggaggcct tgacacccgc ggcgcctatc
     8221 gtccccactt tgcagatata caggctatcg gaggattccg gcctgacggc cgaacaggaa
     8281 tcaccctgac tctatggggc tcaatgaacc gttatacatt cctcccgaga agccagacaa
     8341 ctaccttcgg cacttttgag aatgcctacc gtctgtatgc cttcttcgaa ggtggtgaac
     8401 gcgacagata ctatagcgga ggcggagcgc tcacgcttga gcggaaacac actgaccgcc
     8461 actcttcaaa gctgatcatc tcaaccaacc ttgctgatga gtatgagaac catgacatac
     8521 ggggtgcata cagcctttcg gcccttgaca ggaatttggg aagtgaaaac aatccggata
     8581 ctcttctcaa tgcaggtgcc ggcagttggt tatcacacgc ccgcaactcc ttcaggagtc
     8641 tcatctcagg agtttcatac cgcggctcac tctcttcagg gagaaatggc cttgagtggg
     8701 gtcttgccgt caggcatcgc agctatgact ctttccggaa tgagtggctc agggttgact
     8761 ctgccggtta tacacaaggc cagcaaggtg actggctggc tcttactacc cgcactttac
     8821 tcggcaatga gacccgcagt ttcaccagtg aagggtggct catcagccgc aggcacttca
     8881 cggttgccgg gcgggcgtgt gacctcaacg ccgggataag agtctcatat gatacatact
     8941 gttccgaaat gcttgtctca ccacgcgtat cactgcgcag taagattacc cggcaagttt
     9001 cactctattg tgctgcaggc gcataccacc agcctcccga agtcagggaa ctgatcagtg
     9061 aagatggtga aggcggttct gacccaaagg cacagcgttc ttatcacctg acagccggag
     9121 tacactatga cttcatggcc tgggacagac cattcaggtt caccgcggaa gcctatggca
     9181 aactgtttga caggcttatt ccatacaaga cagataatgt caggctgata tactacggcg
     9241 gcaatattgc cgaaggatac tccgcaggaa ttgacatgcg tgtcaatggt gaatttgtgc
     9301 ccggagttga atcatggttc agcatgtcac tgatgaagtc cgaaatgcag atacccgggc
     9361 tctctaccgg ctggtacccg gctccgttcg accagaggat acacttcagc atcttcttcc
     9421 aggattacct cccggggcat cctgacttca gggcacatat caacgttgcc ttcggaaccg
     9481 gaataccgac ctctcctccg gatcatgaac agtgggatat atggttcaga atgcctccct
     9541 acaggcgtat agatatcggc ttttcgaaag tccttctggg cgccggcaga gccaacaggg
     9601 attctttcat gaaagaactg gtggctggca ttgagatatt caacctttcc gatataagga
     9661 acacaatttc atatacatgg ataaggactg tcagaaacag tcagggagag acgcgggaat
     9721 atgccgttcc gaactatctc acaagaagaa gtctgaatct gaaactgact gcacatttct
     9781 gacgtataaa gaggtcagcg tgactttttt actaatttcg taccctgatg agcaggtttg
     9841 agatcatatt tacggcagtg atggctacaa tcataataca cctgactgta gctgtgatct
     9901 ttgtgtctgt aaagatctct gcactcaagc gcgagatagc cgccgagatc atactgtcaa
     9961 tagatgagga gatgccggat g
//
LOCUS       DAOPBK010000094         9778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6641_length_9778_cov_5.713669, whole genome shotgun sequence.
ACCESSION   DAOPBK010000094 DAOPBK010000000
VERSION     DAOPBK010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6641_length_9778_cov_5.713669"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1465)
                     /locus_tag="PLA17_06770"
                     /pseudo
     CDS             complement(278..1465)
                     /locus_tag="PLA17_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610640.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
     gene            complement(1471..2766)
                     /locus_tag="PLA17_06775"
     CDS             complement(1471..2766)
                     /locus_tag="PLA17_06775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK85154.1"
                     /translation="MRVLMFGWEFPPHIAGGLGTASYGLTRGLASHRDIEVIFVVPKA
                     YGDEDQSMVRLIGANTVPVAFKKVQYKYFRKPVAKIEVFSRLVPYTDPDEFWKMTRGE
                     VADTDVLISTNKHGKVDFTGSYGATLMEEIHKYSVVAQVIASEQKFDIIHAHDWLAYP
                     AGMAAKAVSGKPLVIHVHATDFDRSGGNVNPVVYAMEKEGMDAADKIITVSNLTRDIV
                     INRYNIDPGKVETVYNAVEPLHHSEFDIPGRSYDDKIVTFLGRITMQKGPEYFIEAAR
                     MVLSRMHNVRFVMAGSGDMMERMMRHAAALKITDRFHFTGFLRGNDVFTMLRMSDVYV
                     MPSVSEPFGISPLEAMQSNVPVIISKQSGVAEVLTHAVKVDFWDIEAMADAIYGILTY
                     PALSRMFIMNGKEEVDELKWEKAAGHVKSIYSEVLQQTR"
     gene            complement(2768..4720)
                     /locus_tag="PLA17_06780"
     CDS             complement(2768..4720)
                     /locus_tag="PLA17_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPK85155.1"
                     /translation="MGYIKFDKAHVVNLEYSLTREILRNNRAGSYSSTTIVGCNTRKY
                     HGWLVCPVDAMGGERHVLLSSVDSTVVSNGQSFNTGIHKYQGDHYSPKGHKYVEDFDI
                     QDIPGMTFRVGNVILKQERILVHYEEQLLIRYTIIDTDEPIKFQLRPFLAFRSIHQLT
                     RANMWANTRTEPVPHGVKIRMYDGFPYLHMQLSCKAEFVPVPDWYYGVEYVEEQKRGY
                     DYSEDLFVPGFFEMTASKGDVIVFSVSTREEKYAGLKAKFNRTIATKIPRSNFGNCLR
                     NAAQQFIEKRGNDTNIIAGYHWFGSWGRDTFIALPGLALARESEDLYAAVLDTQVRRM
                     KGGLFPNMGDNDNPAFNSVDAPLWFFQALWNYGLDLKETWQRYGSAMKDVLNAYRAGT
                     SFGIHMRGNGLIYSGAPGKALTWMDAVVEGVPVTPREGYAVEINALWYNAVCYSLDCA
                     RGARDRAFVKEWEKLPELISSSFIETFWDDEQGYLADYVKDGEKRNMQVRPNMVIATS
                     LPYSMLTLDQMKSILDIANRVLVTPRGLRSLSPSDEEYKGIYSGDQATRDRAYHQGTV
                     WPWLLGPFCDGWLRVYGEGGVSRVRKLIMGFEETLTEAGISTISEIFDGDPPHSPRGA
                     ISQAWSVGEILRIFTLLEKNYPDNNK"
     gene            4980..6152
                     /locus_tag="PLA17_06785"
     CDS             4980..6152
                     /locus_tag="PLA17_06785"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPK85156.1"
                     /translation="MGKKVILMLFCVSVLLSGCKRGGFSISGTIDGAGDGEYLLLKEV
                     KPGILKPVDSVIPGKDGRFSFRSETEWPAFFMLSMDDNDFLTMLIEPGEKIEVKAERN
                     SMARPSSVTGSGGTSVILAYRKDQDEVVAGLKRLTDIYNDSIDSPRRPLLMDSLDRCA
                     AGIVADFRERALTLLEENRSSMIPVYLLNQHVVPGLALFEPAKNPELFYSVDSTLYAL
                     YPGSDLVLDLHSFVAGLRKRVSVGRKTGEPPVHGDLLPDILLPDPSGDTLRLSSLRGK
                     VVLVDFWAAWCPPCREENPNLVKLYDMYHWKGFDIFQVSLDLRKEDWLQAIRNDRLGR
                     WKHVSDLRYRDSEVVRQYGLTEIPASFLIDREGRVRAVNLRGNDLQKKLEELLEAQ"
     gene            6200..7360
                     /locus_tag="PLA17_06790"
     CDS             6200..7360
                     /locus_tag="PLA17_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPK85157.1"
                     /translation="MKRFLPVLLAVSLLAGCNNENKVVIKGSFTVPAEGVVYLDQSEV
                     DRNIRVDSAMIKRGQFKFVTEVTGPEFYQLRFTGNEFVALLVMPGENISLEIGKTPVA
                     MNYTVQGSPGSADIRMLDQQLLVTKTRLDSISNIYNSLSANDMDIRGAELQKAYTEVV
                     DAQRKFNIKFVVENISSMSSIQALYQRIDENTYVLYQPRDLQYLKIVSDSLSVRYPVS
                     RHVRALKENVTTELNQMYIDRISSIAMQMPETSSSPELPDINGKMVSVASLKGKYVLV
                     SFWSTTSEESMNQLPVLRSIYNDYHRKGLEIYQISLDPDAERWKNVVRYEEIPWISVR
                     EPDPENPVYARLMNITYLPSNLLYDPQGNIINTNLTGRNLLIRMDQLFNKQR"
     gene            7362..8108
                     /locus_tag="PLA17_06795"
     CDS             7362..8108
                     /locus_tag="PLA17_06795"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPK85158.1"
                     /translation="METRKAYFASDFHLGLAAGTDPRECEQRVTAWLDRVSKDAESVW
                     LLGDVFDFWWEYKKVIPKGFTRFLGRLASLTDSGIKVHVFTGNHDMWMKGYLAEECGV
                     TVHYEPLVTTIGGELFYLAHGEGLGSRRIRERLLFSLFRNRIASILFSAIHPRWGVAI
                     GHAWSKNSRLARHISPPFMGEEKEELIRHTRSVAAAGCRAKYYIYGHRHLPLTFDEDG
                     RRMIILGDWSGCRESYAVYDGKELTLVTVQ"
     gene            complement(8388..9530)
                     /locus_tag="PLA17_06800"
     CDS             complement(8388..9530)
                     /locus_tag="PLA17_06800"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HPK85159.1"
                     /translation="MKLIKKLRKKLSDEHLMFAYRGEVTGDNSVSLLTLLEREMEFSD
                     FSMLGRKRLFMFVLENLQNITRHGISRSEDITSLVVYSKTPDGYTVSTGNALKKSEVR
                     GLRKNLQKINNLDPDKIREVYRTMLQDTKIGKKGGAGLGLMEMARKTGNRLDYDFYPI
                     DDRYSYFILSKTIDSGGKGITTPEQDQGESTFDSKRIMQMERMMKSNNIFFIWAGPMT
                     HAISKELLNFSETTMREAELEQNLHKRVFATLIELLQNVAQHSPGFEAEKHFGIPLAM
                     IRRTPASFIITSGNLIRKSDTDYLTQKLDMVNRYDEEGLKKLLKVALMGQDMSKVSTG
                     YMGLLEMARRSGNRLTYKFDDVNDEYAYYTVTVRVRAKGFKSPVAG"
     gene            complement(9615..>9778)
                     /locus_tag="PLA17_06805"
     CDS             complement(9615..>9778)
                     /locus_tag="PLA17_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85160.1"
                     /translation="AAMEKRGLSARAFERILKVSRTIADLDRSETIRPDHLSEAINYR
                     NLDREGWAG"
BASE COUNT         2355 a         2516 c         2443 g         2464 t
ORIGIN      
        1 ccttcctggc tgctttcccg gcctttgtgg tactcttctt agcggctgcc tccggggcag
       61 caggtttagc ttcggcggca atctttactg tcacggcctt tgtggcaacg ctcctggttt
      121 ttgtggctgc ggcctttggc tttgtttttg cctccatctg cttttcaagc ctggccagct
      181 cctcctgaag ctcgctgatc atctgatcac gtttcttaac atcagctgag agccggttga
      241 tctcctcatc atgcacatcc accggacata atgcattgag tcttatctcg aagtcactca
      301 gcacattcat gtagttcatg aatgcctggt acggattctc ataaggatta aaataggcgt
      361 gaacagcacc gtcagagaag aacttcgttg acatgtagta gaaatggtca ctcgactgca
      421 gataaaccca gtcgcgtatc aactgctcat tattacttct gccaaccctg tcggaaagac
      481 tgtagagctt gtttactgct gccgtctgca gctcgttgcc catccaggcg gtgaggtccc
      541 tctcctcgtc agcccatgag atgggtgacg gaacatgcag aagagatacc ggctgatagt
      601 tgtctgccac ctctgaaacc gtcatgaaat taaatggtgt cctcttcatt attgtttcgg
      661 gcagatgcct gaggaagtca aatattcccg tctccttttt ctgatgctct ccgaaggtct
      721 cgtaatccat aaagatattt accagttcgg aggttgatgc cagtcctttt atccagcctg
      781 catatttctc ggctgtcagg ggatagcttg accactcctt gctggagaag cggaaggcta
      841 tgtcatcact gaggatgtag tttctcagca ggaccttcag cctggggttt atagcattgc
      901 agtacaggta gttgggtgat ttccagccga ggatatgttt ggcgccctcg gtgacaactg
      961 ccttgtaacc cagctctgcc atccagctgc caatctcgtc agaatagata agttcggtat
     1021 tccggatgga agtgggttca tacccgatat actccaacat cttttcacgg tgcaccctga
     1081 cctggagttc atatctctcc ctgctcatga gtgatgccag tgaatgtgag tttgtctcgg
     1141 ccaggaactc aacgcatccc gtttctgcaa gttccctgaa ggaggcgagc acttcggggg
     1201 catataacct gaactggtca ataacgattc cggtcagcga gaaggtgacc ctgaacctgc
     1261 ccttgtgctt ttttatcagg tcaaggatta tcctgtttgt cggcaggtag cagtttatgg
     1321 ctatcttccg caggatagac tcattcatga aatcgtcata gtaatagtgt tcctgcccca
     1381 tctcgaagaa acggtaccgt cttagcctga atggctgatg aacctggaaa taaaggcaga
     1441 ttgccttttt ctgtgatgtg ctcatatcgt ttatcttgtc tgttgcaaaa cctctgaata
     1501 aatactctta acatggccgg cagccttctc ccacttcagc tcgtcaacct cttccttgcc
     1561 attcatgatg aacatccttg agagagccgg ataggtcagg ataccatata tggcatcagc
     1621 cattgcttct atgtcccaga aatcaacttt cactgcatgt gtcagcacct ctgccacacc
     1681 gctctgttta ctgattataa ccggcacatt tgactgcatg gcttcaagcg gagatatgcc
     1741 aaatggttcc gaaactgatg gcataacata tacgtcactc attctcagca tggtaaagac
     1801 atcattgcca cgcagaaaac cggtaaagtg gaacctgtcg gtaattttca gagcagcggc
     1861 atgacgcatc atacgttcca tcatgtcacc cgaaccggcc ataacgaacc tgacgttgtg
     1921 cattcgcgaa aggaccatcc tggctgcctc gataaagtat tccgggccct tctgcatggt
     1981 gatacggccg aggaaggtaa ctatcttgtc atcataggat ctcccgggga tatcgaactc
     2041 ggagtgatgc agcggctcca ctgcattgta gaccgtctca accttccccg ggtctatgtt
     2101 gtatctgttt atgacaatgt cacgggtaag gttgctgaca gtaatgattt tgtcagctgc
     2161 atccatcccc tccttttcca ttgcatatac caccggattg acattccctc cgctgcggtc
     2221 gaagtcagtg gcatgcacat gaatgaccag cggcttgccg gagacagcct ttgcagccat
     2281 ccctgccgga taagccagcc agtcatgcgc atggatgata tcaaacttct gttccgaagc
     2341 aataacctgt gccaccaccg agtacttgtg aatctcttcc atcagggtgg caccgtagct
     2401 tcccgtgaag tcaaccttgc catgcttgtt tgtactgatg agcacatcgg tatcagccac
     2461 ctcgccccgg gtcatcttcc agaactcatc agggtcagta tatggtacca gcctggagaa
     2521 gacctctatt tttgccacag gtttccggaa atacttgtat tgaaccttct tgaaagccac
     2581 gggcacggtg ttggcgccta tcagtcgcac catgctctgg tcttcatcac cataagcttt
     2641 cgggacgacg aagatgacct caatatcgcg gtgggaggcc aggccgcggg tgagaccata
     2701 gctggctgtt cccaatcccc ctgctatatg aggagggaac tcccatccaa acatcaggac
     2761 acgcatatca tttgttatta tctggataat ttttttcaag cagggtaaag atgcggagaa
     2821 tctctcccac gctccacgcc tgtgatattg cccccctggg tgaatgtggc ggatcaccat
     2881 cgaagatttc cgagatggtt gaaatgcctg cttcggtaag tgtctcctcg aaccccatta
     2941 taagcttcct gacgcgtgag acgcctccct cgccgtagac cctgagccat ccgtcacaga
     3001 acggaccgag cagccacggc cagacggtac cctggtggta agcacggtca cgcgttgcct
     3061 ggtcaccgga atagataccc ttatactcct catccgacgg cgacagtgag cgcaaacccc
     3121 tgggcgtgac gaggactctg tttgctatat ccaggatgct cttcatctgg tcaagtgtca
     3181 gcatggaata cgggagtgag gtggcgatga ccatattcgg tctgacctgc atgtttcgct
     3241 tctcaccgtc ttttacataa tcagccaggt aaccctgctc atcatcccag aaagtttcga
     3301 tgaatgagct gctgatcagc tcgggcagtt tttcccactc cttcacgaaa gccctgtcgc
     3361 gggcgccacg ggcacagtca agcgaatagc aaaccgcatt gtaccacagg gcatttatct
     3421 caacggcata gccttcacgc ggagtcaccg gaacaccctc caccactgcg tccatccatg
     3481 tcagtgcctt gcccggagcg cctgaatata tcaggccgtt tcccctcatg tggatgccga
     3541 aggaggttcc ggccctgtag gcattgagca catccttcat ggcagaaccg taacgctgcc
     3601 atgtctcttt aagatcaagt ccgtagttcc acagcgcctg gaagaaccag agaggcgcat
     3661 ccacgctgtt gaatgcagga ttgtcattgt ctcccatgtt tgggaagaga ccacccttca
     3721 tccggcgtac ctgcgtgtca agcaccgccg cgtaaagatc ttctgattcc ctggccagcg
     3781 ccagaccggg caatgcgatg aatgtgtcac gcccccatga tccgaaccag tgataccccg
     3841 caataatgtt ggtgtcattg ccgcgcttct cgataaactg ctgtgcggca ttgcgaaggc
     3901 agttgccaaa attgctcctc ggaatcttgg tggctatggt gcggttgaat ttggccttca
     3961 gccctgcata cttctcttcc ctggttgaaa cggaaaagac tataacgtct ccctttgaag
     4021 cggtcatctc gaaaaagccc ggaacaaaca gatcctccga atagtcatag ccgcgcttct
     4081 gctcctcaac atactcaacc ccgtagtacc agtcaggtac cggcacgaac tcagccttgc
     4141 atgacagctg catatgaaga taagggaagc cgtcatacat cctgatcttt acaccgtgtg
     4201 gcaccggctc ggtacgggtg ttggcccaca tgttggcacg ggtcagctgg tgaatgctcc
     4261 tgaaggccag gaatggcctc aactggaact ttataggctc atccgtgtca atgatggtat
     4321 accttataag cagctgctcc tcgtaatgaa ccaggatgcg ctcctgctta aggattacat
     4381 tgcccacccg gaaagtcatc ccgggaatgt cttgaatatc aaagtcctcc acatatttgt
     4441 gccccttcgg gctgtagtgg tcgccctgat acttgtggat acctgtgttg aaactctgac
     4501 cgttactgac tacagtggag tctaccgatg atagaagaac atgcctttcg cctcccatgg
     4561 catcaacagg gcataccagc cagccgtgat atttgcgggt attgcatccg actatggtag
     4621 tggacgaata gcttccggcc ctgttgttcc gcagaatttc gcgggtcagt gaatattcca
     4681 ggtttactac atgtgccttg tcaaatttaa tgtatcccat attttctgga gttccgtttt
     4741 tactgctaaa gaaaagcagg agaattaact ctaataataa taacaaatct cttcacgtgc
     4801 aaagaaaagc aaaaaaaagg gattaattat gtgccggccg ttggtatgcc tctccataaa
     4861 ggctctgaaa aaaaaatatc tttgcattgc tgaaggagaa ccaatgcaag gcgagatggt
     4921 ttgaacggaa taatggtaat ttaatatggg ccttgaaaat ttaatatata ttttacaata
     4981 tgggtaaaaa agtaattctg atgctgtttt gtgtgtctgt tctgctatcc ggatgcaagc
     5041 gcgggggctt cagtatcagc gggactatag atggtgccgg agatggagaa tatctgttgc
     5101 tgaaagaggt taaacccggc atcctgaaac cggtcgactc ggtgataccg ggcaaggacg
     5161 gcaggttcag cttcagatcg gagactgagt ggccggcatt cttcatgctc agcatggatg
     5221 acaatgactt tctcaccatg ctcatcgaac cgggagaaaa gattgaggtg aaggctgaac
     5281 ggaattcaat ggccagacca tcttcggtta caggatccgg gggcacttca gttatcctcg
     5341 catacaggaa ggaccaggac gaggtggttg ccgggctgaa gagactcacc gatatctaca
     5401 atgacagtat tgacagcccc cggcgtccgc tgctgatgga cagcctcgac aggtgtgccg
     5461 ccggtattgt ggccgacttc cgtgaaaggg ctttgactct ccttgaagaa aacaggtcat
     5521 caatgatccc cgtttacctg ctgaaccagc atgtagtgcc gggactggct ctttttgaac
     5581 cggcaaagaa ccctgagctc ttttatagtg tcgattccac attgtatgcc ctttaccccg
     5641 ggtctgacct ggtgctagac ctgcattcgt tcgtggcagg tctgcgcaag agagtctccg
     5701 tgggcagaaa gaccggagag cctccggtgc acggtgatct gctccctgac atactcctcc
     5761 cggacccttc cggtgacacg ctcagactgt catccctgag aggaaaggtt gtgctggttg
     5821 atttctgggc tgcatggtgc ccgccatgcc gcgaggagaa ccccaatctt gtaaaactat
     5881 atgatatgta ccactggaag ggatttgaca tcttccaggt atcacttgac ctcaggaagg
     5941 aggattggct ccaggcaata agaaatgacc ggctgggacg gtggaagcat gtgagcgacc
     6001 tccgttaccg cgactcggaa gtggtcagac agtacggcct taccgaaatc cccgccagct
     6061 tcctcattga ccgtgaagga agagtgagag cggttaacct caggggcaat gaccttcaga
     6121 aaaagctgga ggaactgctg gaggctcagt agccagtgcc gatttttagt ttaatttgta
     6181 cccgaaatat caaaaaggaa tgaagagatt tttacctgtg ctcctggccg tatctctcct
     6241 ggcaggctgc aacaacgaaa ataaagtggt aataaagggt tcctttaccg tgccggctga
     6301 aggtgtggta taccttgacc agtctgaagt tgaccggaat ataagggtcg actcggccat
     6361 gataaaaaga ggacagttta aattcgtaac cgaggtgacg ggtccggagt tctaccagct
     6421 gagattcact ggcaatgagt tcgtggcgct gctggtaatg ccgggcgaga atatttccct
     6481 cgaaattggg aaaacaccgg ttgcaatgaa ttacaccgtg cagggttcgc cgggatcggc
     6541 agacatacgg atgcttgacc agcagctgct ggtgacaaaa acaaggctcg attcaataag
     6601 caatatctac aactccctca gcgcaaatga tatggatatc cgcggagcgg agcttcagaa
     6661 ggcatacacg gaagttgtag acgcacaacg caaattcaac atcaaatttg tggtggagaa
     6721 tataagctca atgtcatcaa tacaggctct ttaccagagg atcgacgaaa acacatatgt
     6781 cctttatcag ccccgcgacc ttcagtacct gaagatcgta tctgattcac tttcggtccg
     6841 ctatccggtt tcgcgccatg tgcgcgcact taaggaaaac gtcaccacgg agctgaacca
     6901 gatgtacatc gaccggatct catccattgc catgcaaatg ccggaaacca gttcatcacc
     6961 ggaacttcct gacatcaatg gcaaaatggt cagcgttgca tcactcaagg gaaaatatgt
     7021 gctggtctca ttctggagca ccacctcgga agaatcgatg aatcagctgc cggtactcag
     7081 gtctatctat aacgactatc acaggaaagg actggaaatc taccagatca gtcttgaccc
     7141 tgatgccgaa cgatggaaaa acgtggtacg gtacgaggag ataccatgga tcagcgtcag
     7201 ggagcctgac ccggaaaatc cggtttatgc acggctgatg aatatcacat acctcccctc
     7261 aaacctgctt tatgacccgc agggcaatat aataaacacc aacctgacag ggcgcaatct
     7321 gcttatcagg atggaccagt tattcaacaa acagaggtga tttggaaacc cggaaagcat
     7381 attttgcctc tgactttcac ctgggactgg ccgcagggac tgatccccgt gaatgcgagc
     7441 agagagtgac agcatggctt gaccgggtgt caaaagatgc agagtctgtc tggctgctgg
     7501 gcgacgtgtt tgatttctgg tgggagtaca agaaggtgat cccgaaagga ttcacaaggt
     7561 ttctgggcag gcttgcctct ctgactgaca gcggaataaa ggtacatgtc tttaccggta
     7621 accatgatat gtggatgaaa ggctacctgg ccgaagaatg tggcgttaca gtacattatg
     7681 aacccctcgt cacaaccatc ggaggagagc tgttttatct ggcccatggc gaaggactcg
     7741 gaagcagaag gatcagggag aggctgctct tcagcttgtt ccggaacagg atcgcgagta
     7801 ttctcttctc tgccatccac ccgcggtggg gtgtggctat aggacatgca tggtcaaaaa
     7861 acagcaggct ggcaaggcat atatctcctc cattcatggg tgaggagaag gaagagctca
     7921 tcaggcatac aagaagtgtt gcagcggcag gatgcagggc gaagtattac atctacggtc
     7981 acaggcacct gccactgaca tttgatgagg atgggagaag gatgataatc ctgggcgact
     8041 ggtccggctg ccgtgagagt tatgccgtgt atgacggaaa ggaactcacg ctggtcactg
     8101 tgcaataaga ttcctgtgcc ggcagcgccc cggccatcct gtcttgagat tcctgtgcgg
     8161 gaagggccgg acaccctcca atgatattcc cgtgccggca gcgccggttt tcctgcaatg
     8221 atattcctgt accggcagca ctggacaccc tgcaatgaga tttctgtgcc ggtcaagcct
     8281 cagccaccct gcaatgagat tcccgagccg gcagcgtcgg acactcagta atgagatttc
     8341 tgtgccggca ccgccggaca cccggacaat caaaagatta attcagatta acctgccacg
     8401 ggcgacttga atcctttggc cctcaccctg acagttacgg tgtaataggc atattcgtca
     8461 ttcacatcat cgaacttgta ggtaagcctg tttcctgacc tgcgggccat ctccagcaga
     8521 cccatatagc cggtggaaac ctttgacata tcctgtccca tcagtgccac cttgagcagt
     8581 ttcttaagcc cctcctcatc atacctgtta accatgtcaa gcttctgtgt aaggtaatca
     8641 gtgtcgcttt tcctgatcag gtttcccgag gtgattatga atgatgccgg tgttctccgt
     8701 atcatggcga gggggatgcc gaagtgcttc tcagcctcaa agcccgggct gtgctgtgcc
     8761 acattctgaa gcagttcaat gagtgtggca aaaacgcgct tgtggagatt ctgttccagt
     8821 tcggcctcgc gcattgttgt ctcgctgaag ttcagcagtt ccttgctgat ggcatgggtc
     8881 atgggccctg cccagataaa aaagatattg tttgacttca tcatccgctc catctgcatt
     8941 atccttttag agtcaaacgt gctctcgccc tggtcctgtt cgggcgttgt aatcccctta
     9001 ccgccggaat caatggtctt gctgagaatg aaataggaat acctgtcatc tatcgggtag
     9061 aaatcatagt caagcctgtt tcccgtcttg cgggccatct ccatcaggcc cagtcctgcc
     9121 ccgcccttct tgccgatttt ggtatcctgg agcatggttc tgtagacctc ccgtatcttg
     9181 tcaggatcaa gattgttgat cttctgaagg ttttttctga gccccctgac ctcgctcttt
     9241 ttgagcgcat tgcccgtgct gacggtgtag ccgtcggggg ttttgctgta aacaaccagg
     9301 gaggtgatgt cctcactccg ggagatgcca tgcctggtta tattctggag gttttcaagg
     9361 acgaacatga agagccgttt gcgtcccagc atgctgaagt cagaaaactc catctcacgt
     9421 tccagcaggg tcagaaggct gacggagtta tctcctgtca cctctcccct gtaggcgaac
     9481 atcaggtgtt catctgaaag cttcttccgt aatttcttaa tgagtttcat acaatccgac
     9541 aaaatgaagt atcaatattc ccatgagaca aaataaataa aaagaaatgt cacaagctat
     9601 agttaaaatg aatttcagcc tgcccagccc tcacggtcga ggtttctgta attaatggct
     9661 tcggaaaggt ggtcaggcct gatggtttct gatcggtcga ggtcagcaat tgtacgggat
     9721 accttaagaa ttcgttcaaa ggcgcgggcg gagagtccgc gtttctccat cgctgccc
//
LOCUS       DAOPBK010000095         9733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6674_length_9733_cov_6.166977, whole genome shotgun sequence.
ACCESSION   DAOPBK010000095 DAOPBK010000000
VERSION     DAOPBK010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6674_length_9733_cov_6.166977"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLA17_06810"
     CDS             <1..522
                     /locus_tag="PLA17_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK85161.1"
                     /translation="IELRRKVGMVFQRPNPFPNMTIYENVVAGFSLNDIRMKKSRRDE
                     IVENALRNVTLWDEVKESLYRKGTFLSGGQQQRLCVARAIAFNPEVLLLDEPTSALDP
                     IATLKIEDLLGELKKKYTMVLVTHNMSQAARISDDSMFMYLGELVESGRTGTLFTTPK
                     DPRTEAYLTGKFG"
     gene            562..1257
                     /locus_tag="PLA17_06815"
     CDS             562..1257
                     /locus_tag="PLA17_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPK85162.1"
                     /translation="MSEKKEQALNEIKSSFDEMASLVLRQLTLLDKLMGATEEQAFTD
                     LIVEMDKAESRIDNLEVVIGEQFTSTIVLYQPVASDVRRLVAIYRMSINLERIGDRIH
                     NLCRIIRDIRNTTEFSSTASLVTNMLTSGTIMVEKALLSFTNSDPDYAIWTIKNDTVV
                     DEMNRKLLLCHLRKENIDNKTRSMVQCYIELKDMISNIERIADHATNIAEASIYSLQG
                     TDIRHTGIGKEEK"
     gene            1392..2069
                     /locus_tag="PLA17_06820"
     CDS             1392..2069
                     /locus_tag="PLA17_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPK85163.1"
                     /translation="MNERRKILVVDDEEPILEILSFNLEGEGYQVDTCLSAEEAMRKN
                     LRSYDLFILDVMMGLMSGFRLAEKIRKENDLSTPIIFLTARDTENDKITGFNLGADDY
                     LAKPFSVRELMARVKAVLRRSAPARVTLETVAGSFTVDTENNLILSDGKVLELTRKEH
                     DILSLLIRNEGKIFPRHEILSRVWGDDVIVTDRTVDVTIARIRKKLGKMGNMIRNKSG
                     YGYYLGE"
     gene            2094..3842
                     /locus_tag="PLA17_06825"
     CDS             2094..3842
                     /locus_tag="PLA17_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPK85164.1"
                     /translation="MSFRRRLFLYYFGIFLLFTVLILAFQYHREKDIKIAALDNRLND
                     IAQLTDNYIKVNSLNVTGNYHLIDSIFNLIPVPDLRITILAIDGKVLYDSSVDNWPAM
                     ENHLDRPEVGKSLYSPYGTAIRHSESTGKDYYYFSRYFNTHYIRIAEEYNINIKGFLK
                     RETTFLVFMVIIFAGIWALLAAVTKRMGKSITMLRDFAARMRRGEGNDPSIIFPKNEI
                     GEIGKEIVRIYNNLALNTAELSLQKEKLIRHLHVLNEGVAFFTPERTVTLSNSHFMVF
                     LNTITGELSLTPEGFLDHPEFARIKAFLNSNLETGAGKQENPSTEMRIEKGGKYFSVR
                     CILFNDESFEVVITDITSTEQNKAMRQQMTSNIAHELKTPIASVRGYLETLIVNKDLD
                     EHKRNHFLEKALGQSARLTDLINDIVTLNKLDETGTYFPFDEVDIADVVKEVCDNLMA
                     DIRSKQIKLDNAIEPGTMVNGNRSLIVSVFQNLLENVIAYAGEKVTVTVRTLPGEPGF
                     RAFSFADNGVGIPAEHQERIFERFYRIDDGRSRKNGGTGLGLAIVKNAVLLHRGEISV
                     RTRPGGGAEFIFSLPA"
     gene            complement(4104..5141)
                     /gene="selD"
                     /locus_tag="PLA17_06830"
     CDS             complement(4104..5141)
                     /gene="selD"
                     /locus_tag="PLA17_06830"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPK85165.1"
                     /translation="MLFDLLTTVETGGCSAKIPPRQLEELLKDMPLPADPRVMVDIAT
                     HDDAGVYKINDETALVITTDFFPPMVSDGYEFGLIAAANSISDVYAMSGTPLVALNIM
                     MFPSTRIPMEVYKNILRGGLDKAREAGVSIIGGHTIDDWPPKYGLAVIGTVHPGRVIT
                     NAGVREGDELILTKPLGTGIILAGQRMKMITPLQHEHAVSLMKQLNARSAELMQKYGV
                     RGATDITGFGLAGHALRMARGSGVTITIDMKSVPLLEGAYSLADLGCIPGAAFRNLDY
                     TEGGTLFAGDLDYNLKMIAHDAQTSGGLLMAVSPEKSRELLKELHESGYGGAALIGKA
                     IRKREADVVLQ"
     gene            complement(5305..5925)
                     /gene="yedF"
                     /locus_tag="PLA17_06835"
     CDS             complement(5305..5925)
                     /gene="yedF"
                     /locus_tag="PLA17_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPK85166.1"
                     /translation="MKTVDTRGLTCPAPLIKTRQGLVEAAPDEDVEILIDNPTSLSNV
                     KRYLSDNKLPFTIRGEGSLSIVTVRRGEKAELSPNEKEYCTASAEEPAPGGRKTVVAV
                     ASDRMGSGDDDLGTKLMLSFFRTLVMLEPAPAAVVFYNTGVKLAMDDSPALEHIRELA
                     EKGTSIFICTTCINHFGIRERLPVGSFSDMYQIVNILRDADHIIRP"
     gene            6084..7502
                     /locus_tag="PLA17_06840"
     CDS             6084..7502
                     /locus_tag="PLA17_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPK85167.1"
                     /translation="MKRTALLIGLFIITGMLLSGQRTSRDLEGAWSGKLKTPSVELQI
                     VFRFTITEADSIKAVLDSPDQGAFDIPCGEVTFQEDTLFVDIPIVQGSYRGGFGSDTL
                     ISGTFTQMGIKYKLSITKGAKPVVYNRPQEPTPPYPYREEEVTFMNTVENFSMAGTLT
                     LPQGEGPFPAVVLISGSGQQDRDETLFMHKPFKVLADHLTRNGIAVLRYDDRGMGKSK
                     GNALKATSLSFADDAEAAVTYLMEHPEINVSKIGLAGHSEGGLIAPIVASRNRNIAFI
                     VSLAGTGVTGYDIILRQTADIMKASETAQAEIDETLAVNGQLFRMLVDEPDQRKFPKD
                     AMEWYGRELDKKGLTQEERQQKMAEFTRGLLSVNNPWMRCFLATDPALFWSQVKCPVL
                     ALNGDKDLQVSHEVNLPAIRAALRSGGNRKVKTVILPGLNHLFQHSETGSPNEYVTIE
                     ETFSPEAMELITSWIRKTMKMR"
     gene            complement(7537..7734)
                     /locus_tag="PLA17_06845"
     CDS             complement(7537..7734)
                     /locus_tag="PLA17_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPK85168.1"
                     /translation="MKSKRQKHSGAFKAKVVIEALQEQESFSELSVKYGILPNMSSRW
                     KKEFPDRAPELFDKQVQIMRA"
     gene            7847..8032
                     /locus_tag="PLA17_06850"
     CDS             7847..8032
                     /locus_tag="PLA17_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85169.1"
                     /translation="MIIKNFDIDGALTNKAGIKDLKEIITDFGKQQGVSKVIIEGAKR
                     TTGVNPGRIPSQLIFDI"
     gene            complement(8284..9639)
                     /locus_tag="PLA17_06855"
     CDS             complement(8284..9639)
                     /locus_tag="PLA17_06855"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HPK85170.1"
                     /translation="MIKNYSETTPIVHELFSVSNKQIELSFTGYDISSDGGLLLLREV
                     ENQMGLINRISNCITDNRDQRYVDHTIREMLTQRVFQIAAGYEDCNDCDDLRDDMIFK
                     TCAGRLPQTGLSLASQPTMSRLENSIKSQDLYRIGQELVDTFVESYDREPGVIILDCD
                     DTNNNTYGQQELNLFNQYYHDHCYMPLHIYEGLSGKLITTILKPGRRSKQSDIASLLK
                     KLILHLRKEWANTMIIVRGDSHFTSADFMRWCEEQYNTGYITGVSGHRKLHEMAAVTI
                     QSAEREFKQYGKYVKRYHSFLYQAGSWSQPQKVVVKVEVSAMGTNIRYIVTNLVQFRA
                     KDLYEKGYCARGAMELRIKEHKLYLRSDRSSCHRFKANQFRLFLHSIAYVLLHTFQKE
                     VLRGTEFENATFKTIQNKIIKTAAWVKEMKTKVKIELPRCCPTKSIQSNCLEMFAVMR
                     V"
BASE COUNT         2570 a         2400 c         2321 g         2442 t
ORIGIN      
        1 atagagcttc gccgtaaggt gggaatggtc ttccagcgcc ccaacccttt cccgaacatg
       61 actatctacg aaaatgtggt ggccggattc agcctcaacg atatacgtat gaaaaaaagc
      121 cggcgtgacg agatagttga aaatgctctg cgcaacgtta cactctggga cgaagtgaag
      181 gagtcgcttt acagaaaagg aacattcctc tccggcgggc aacagcagcg gctgtgtgta
      241 gccagggcca tcgcttttaa ccccgaggtg ctgctcctcg acgagcccac ctcagccctt
      301 gaccctattg ccacattgaa gattgaggac ctgcttggcg agctgaagaa gaaatatacc
      361 atggtgctgg tgacccataa catgtcacag gcagcccgta tatcagatga ctcgatgttc
      421 atgtacctgg gagagctggt ggagtcgggc cgtaccggga cgctctttac caccccgaaa
      481 gacccgcgta ctgaagcata tctgaccgga aaattcggat gattagcgag gttttttata
      541 catttgctaa aacgcttgat tatgagtgag aaaaaagaac aggcactgaa tgagataaag
      601 agcagctttg acgagatggc aagtcttgta ctgaggcagc tgaccctcct ggataagctt
      661 atgggcgcaa ctgaagaaca ggcattcact gatctgatag ttgagatgga caaggctgag
      721 agccgcattg acaaccttga ggtggttatt ggcgagcagt ttaccagtac cattgtgcta
      781 tatcagccgg tggcctctga cgtgcggcgc ctggtggcca tataccggat gtctattaac
      841 ctcgagcgca tcggagacag gatacataac ctctgccgca ttattcggga tatcagaaac
      901 acaactgagt tttcctcaac tgcctctctg gtcacaaaca tgctcacctc gggaaccatc
      961 atggtggaga aggcgctgct ctcattcacc aactctgacc ctgattatgc catatggaca
     1021 atcaagaatg acacggtggt tgatgagatg aaccgcaagc tgctgctttg ccacctgcgc
     1081 aaggagaaca ttgacaacaa aacacggtca atggtgcaat gttatattga gctgaaggat
     1141 atgatatcca atattgagcg gatagctgat catgccacca acattgcaga ggcatcgatc
     1201 tactccctgc agggaacaga tatcaggcat acaggtatcg ggaaggagga gaagtgaagc
     1261 cggctgtcag caataccagc cggcaattca agactgcgct gaagactcag tcacgacccg
     1321 ccatccggcg aagggggacg gcagttgaag gcttgaccga agggcataca gcacagcata
     1381 aggcattgta aatgaatgaa cgaaggaaaa tactggttgt agacgacgag gagcccatcc
     1441 ttgaaatcct ctcatttaac cttgagggtg aaggctacca ggttgatacc tgtctttcgg
     1501 ccgaagaagc gatgcgcaaa aacctccgca gctatgacct gttcatcctc gatgtgatga
     1561 tgggccttat gtccggcttc cgcctggcgg aaaagataag aaaggaaaat gacctctcaa
     1621 ccccgataat cttcctcaca gcaagggaca cggagaatga caagatcacc ggcttcaatc
     1681 tcggagctga tgattacctt gccaaaccat tcagtgtcag ggaactgatg gcacgggtga
     1741 aagccgtcct ccgccggtct gcaccagccc gggtgaccct ggagacagtg gccggctcct
     1801 tcaccgttga cactgaaaac aacctcatcc tctcagatgg caaggtgcta gagcttaccc
     1861 gcaaggagca cgatattctc tcactcctca tccggaatga gggcaagatt tttcccaggc
     1921 atgagatact gtcaagagtc tggggcgatg acgtcattgt tacagaccgt accgttgacg
     1981 tgaccattgc ccgtattcgt aaaaagctgg gcaaaatggg taacatgatc agaaacaaat
     2041 cagggtacgg ttactattta ggcgaatgaa aaaccagtca aatgacaaat ccgatgagct
     2101 tcaggcgtag actgttcctt tactattttg ggatcttcct cctctttacg gtccttatcc
     2161 tggcattcca gtatcaccgt gaaaaagaca tcaaaatagc agcacttgac aacaggctga
     2221 atgacatagc tcagctgaca gacaactaca taaaggtcaa ttcgttaaat gtaacaggca
     2281 actatcacct gattgactcg attttcaacc tcattccggt accggacctg cggatcacga
     2341 tattggcaat tgatggcaag gtgctgtatg acagttcggt agacaactgg ccggcaatgg
     2401 aaaatcatct cgacaggccc gaggtgggaa aatcgctcta ttcaccctac ggcactgcaa
     2461 tccggcactc ggaatcgacc ggcaaggact attattactt ttcgaggtac ttcaatacac
     2521 actatatcag gatagccgaa gaatacaata tcaatatcaa ggggttcctg aaacgtgaaa
     2581 cgacttttct cgttttcatg gtcattatct tcgcaggtat ctgggcgctg cttgctgcag
     2641 tgacgaagcg gatgggaaaa agcattacca tgctccggga ctttgcagca aggatgcgtc
     2701 ggggtgaagg caatgacccg tcaattatct tcccgaagaa tgaaatcggc gagataggca
     2761 aggagatagt cagaatctac aataatctgg cactcaacac cgctgaactg tcactccaga
     2821 aagaaaagct catcaggcac ctccatgtgc taaatgaggg agtggcattc tttactcctg
     2881 aacgcactgt aacactctca aacagccatt ttatggtgtt cctgaacacc attaccggag
     2941 aactctcact gacacccgag ggtttccttg atcacccgga attcgcaagg atcaaggctt
     3001 tccttaacag caacctcgaa accggggccg ggaaacagga gaatccgtcc accgagatgc
     3061 ggatagaaaa aggaggcaaa tacttctccg tgagatgtat ccttttcaat gatgaaagct
     3121 tcgaggttgt cattacagat attaccagca ctgagcagaa caaggcgatg aggcagcaga
     3181 tgacatccaa catcgcccat gagctcaaga ctccgattgc ctcggtcagg ggttacctgg
     3241 aaacactgat tgtgaacaaa gacctggatg agcataaacg taatcatttt ctcgaaaaag
     3301 ctcttggcca gtctgcccgc ctcactgatc tgatcaatga cattgttacc ctgaacaagc
     3361 ttgatgagac aggcacctac ttcccgtttg atgaggttga cattgcagat gtggtaaaag
     3421 aggtctgtga caaccttatg gctgatataa ggagcaaaca gattaaactt gataatgcca
     3481 ttgaaccggg caccatggtc aacggaaaca ggtcgcttat cgtatcggta ttccagaacc
     3541 tgcttgagaa tgtcattgcc tatgccggtg agaaagtcac cgttacggtc aggactcttc
     3601 ccggtgaacc cggtttccgc gccttctcct ttgctgacaa tggcgtcggt attcccgcgg
     3661 agcaccagga gaggattttc gagcggtttt acaggattga cgacggccgt tcacgcaaaa
     3721 acggaggcac aggtctcggg ctggccattg tcaaaaatgc ggtattgctg cacaggggag
     3781 agatctctgt caggacacgc ccgggtggcg gagcagaatt catcttctcg ctgccggcat
     3841 agagaggacc tgttcagcct gcagacgatg tcactccgga agcatcagat gtcgcccact
     3901 gcagcctctt gttgttactc agaagtaatc tgaacgccgg gggataggtc agctatcact
     3961 cactgcagcc ttttgttgtt acttataagt aatctgagcg tgggggtgct gcgtcagtta
     4021 tcaccattgc tgcagttttt gttgttactt gtaagtaatc agaatgccgg gggatgcgtt
     4081 ggttctcacg atcactgcag tttttattgc agcacaacat cagcctccct cttcctgatt
     4141 gctttgccga tgagtgccgc ccctccgtag cctgactcat gcagttcctt cagcagttca
     4201 cggctcttct ccggagaaac agccataagc agtccgccgg aagtctgggc atcatgagca
     4261 atcatcttca ggttataatc aagatccccg gcaaacagcg ttcctccctc ggtgtaatcc
     4321 aggttgcgga acgccgcacc tggtatgcag ccaaggtcag ccagactgta agcaccctcc
     4381 agcagcggca cgcttttcat atcaatagtt atagtcactc cggagcctcg tgccattctg
     4441 agggcatggc ctgcaaggcc gaagccggta atgtcagtgg cacccctgac accgtatttc
     4501 tgcatcaact ctgcactacg ggcgttgagc tgcttcatga ggctcacagc atgttcatgc
     4561 tgcaaaggtg ttatcatctt catcctctgt cccgccagga tgatgccggt accaagtggt
     4621 ttggtcagta tcagctcatc cccctccctt accccggcat tggtgataac cctgccggga
     4681 tgcaccgtgc caatcactgc cagcccgtat ttcggaggcc aatcatcgat ggtatgccct
     4741 ccgatgatgc tgacgccggc ctcgcgtgcc ttgtcaaggc ctcctctcag aatgtttttg
     4801 taaacctcca tcggaatccg ggttgaagga aacatcatta tgttcagcgc taccaggggt
     4861 gttccgctca tggcgtaaac atcacttatt gagtttgctg cagctatgag gccaaattca
     4921 taaccgtcgc tcaccatcgg cgggaagaag tcggttgtga taacgagggc tgtctcgtca
     4981 ttgattttgt aaacacccgc gtcatcatgg gttgcaatat ccaccatgac acgcggatct
     5041 gctggcagag gcatatcctt cagcagttcc tcaagctgtc ggggaggaat ttttgccgaa
     5101 caaccccccg tctctaccgt tgtaagaaga tcaaataaca ttgtatactc ctctgtttaa
     5161 attgatcagg ctgcaagcaa ccaaaagcat tcaccgctat tattgtctgt cacagctaaa
     5221 ctgttcctcc tgacagaccc aggcaccagg atggacgaga ctgcccctga gaagtcacag
     5281 ctgaactgcc ccttccgaca tgcatcacgg acgaattata tgatccgcat ctctcaatat
     5341 attgacaatc tgatacatat cactgaaact gcccaccggc aggcgctccc tgataccgaa
     5401 gtgattgata caggtagtgc aaatgaatat tgatgttccc ttttctgcca gctctctgat
     5461 atgctcaagc gccggtgagt cgtccattgc cagctttact cccgtgttat agaagacaac
     5521 cgctgccggt gccggctcaa gcatcaccag ggtcctgaaa aaggaaagca tcagctttgt
     5581 ccccagatca tcatcgccgc tgcccattct gtccgatgca acagccacta ccgttttcct
     5641 gcctcccggt gccggctcct cagcacttgc tgtacagtat tccttctcgt tcggacttaa
     5701 ctccgctttt tcgcctcttc tgaccgtcac aattgacagg cttccctccc cgcggatggt
     5761 gaacggaagt ttgttatccg acaggtacct cttcacatta cttaaggagg tgggattatc
     5821 tatcagtatc tccacatcct cgtccggagc tgcctcaaca agcccttgtc tcgtttttat
     5881 cagcggggcc gggcatgtaa ggccccttgt atctacagtt ttcatatgct cttaaaatta
     5941 cttcaaatat aaccagaaat agtacctttg agtttcaact ggtatggttt ggtgaaacca
     6001 gtataaacag gaacccacag attgttttga ttgaaaagtc agggtcagct tcaccggacc
     6061 ggtactctaa aactcataaa aggatgaaaa gaacagcttt gctaattggt ttgtttatca
     6121 taaccggaat gttactctca ggccagcgga catccaggga tctcgaaggc gcctggtcag
     6181 ggaaacttaa aacaccctct gtcgaactcc agatagtatt ccgtttcaca attacggaag
     6241 ccgactccat caaggcagta ctggactccc ctgatcaggg tgcttttgat atcccatgcg
     6301 gggaagtcac atttcaggaa gacacgctgt ttgttgacat tccgatagtc cagggtagtt
     6361 accggggtgg gttcggcagc gacacgctga tatccggaac cttcactcag atgggcataa
     6421 aatataagct gtcaatcact aaaggagcaa agccggtcgt ttacaaccgt ccgcaggagc
     6481 cgacgcctcc ctacccctac cgtgaggagg aggtcacgtt catgaacact gttgagaact
     6541 tcagcatggc gggaacactc acccttccgc agggcgaagg accgtttcct gccgtggtgc
     6601 ttatctctgg ctccgggcag caggatcgtg atgagacact tttcatgcat aagcctttca
     6661 aggtgctggc tgatcacctg accaggaatg gaattgcggt gctgcgttat gatgaccggg
     6721 gcatgggaaa atcaaagggc aacgcactca aagctacctc tttgtctttc gctgatgacg
     6781 cagaggccgc ggtgacatac ctgatggaac acccggagat aaatgtcagt aagataggtc
     6841 ttgcaggcca tagcgagggc ggactgattg ccccgatagt agcatcacgc aacaggaaca
     6901 ttgccttcat cgtctccctt gccggcacgg gtgtgacggg atatgatatc atcctcagac
     6961 agactgccga tatcatgaaa gcctcggaaa cagcacaggc tgaaattgac gagaccctgg
     7021 ctgtcaatgg ccagctcttc cgcatgcttg ttgacgagcc tgatcagcgc aagtttccga
     7081 aggacgccat ggaatggtat ggccgggagc tggacaaaaa aggccttaca caggaggagc
     7141 gccagcagaa aatggcagag ttcaccagag gattgctgtc agtaaataat ccatggatgc
     7201 gctgtttcct tgccaccgac ccggccctct tctggtctca ggtgaaatgt ccggttcttg
     7261 ccctcaatgg agataaggac ctgcaggtga gccatgaggt aaacctgccg gcaatcaggg
     7321 ccgcattgcg aagcggcggt aaccgcaagg taaagacagt tattcttccc ggcctgaatc
     7381 acctgttcca gcattcggaa acaggctcac cgaatgagta cgtcacaatt gaggagacct
     7441 tctcacctga ggcaatggaa ctaattacat catggatcag gaaaaccatg aagatgagat
     7501 agagttaaaa gtggaaagtg gaaagtgaaa agtagatcag gctctcataa tctgaacttg
     7561 tttgtcaaaa agctctggtg cacgatccgg aaactccttc ttccaacgtg agctcatgtt
     7621 cggaagaata ccatacttca cggaaagctc actgaaggac tcttgttcct gtaatgcttc
     7681 aattaccact ttcgccttga aggctccgga atgtttttgt cttttcgatt tcattataca
     7741 ctggttttaa gttgtcaaat acaaccttaa ccagtggtct taattcaggg gagtattata
     7801 aaaaaccata gagtttggcg ggaatattac aaaatctaaa ggtgttttga taattaaaaa
     7861 ctttgatatt gatggggcat tgaccaataa agcaggcatt aaggacctga aggaaattat
     7921 tacagatttt ggaaagcaac aaggagtatc aaaagttata attgaaggag ccaaaagaac
     7981 aacaggagtt aatccaggtc gtatcccttc tcaattgata tttgatattt aatatttaac
     8041 ctgattaatt cataaactta caatttaggc attccaatca gatttgtttt ccctttcaag
     8101 ttgtatatgc cgggattccc cggccggttt gctctggatt agctgaacaa gtgtacagat
     8161 ttatcatcta agggtagtga ttttgctaaa aatcagtcga tttcaatata tgtgagcata
     8221 tttttccctg acaacttgtg ttgttctcat ttatatgtaa gggaatcaaa ggattgatac
     8281 tattcagact ctcattacag cgaacatctc caggcaattg gattgaatgg attttgtcgg
     8341 acaacacctg gggagttcta ttttcacctt ggttttcatc tctttgaccc aggctgctgt
     8401 cttaatgatc ttgttctgta tggttttaaa cgttgcattc tcaaactcgg ttcccctgag
     8461 cacttctttc tggaaggtat gcagcaacac ataagctatc gagtgcagga acagacggaa
     8521 ctggttggcc ttaaaacgat gacatgatga gcgatcgctc ctcagataca gcttatgctc
     8581 tttgatgcgg agttccatgg cacctcgggc acagtagcct ttttcataca gatcttttgc
     8641 cctgaactga accaggttgg taacgatata ccggatattg gttcccattg cggagacctc
     8701 gaccttgacg accacctttt gtggctggga ccagcttccg gcctggtaca ggaaagaatg
     8761 gtagcgttta acgtacttgc catattgctt aaattccctt tctgcactct ggatggtcac
     8821 cgctgccatc tcatgcaatt tccggtgacc actcaccccg gtaatgtaac cggtattgta
     8881 ttgttcttca caccaacgca taaaatccgc agaggtaaaa tggctatcgc cacggacaat
     8941 aatcatggta ttggcccatt ctttccgaag atgaaggatc agctttttca acaaagaggc
     9001 aatatcactt tgcttactcc gtcttccggg tttaaggatt gtggtgatca gcttgcccga
     9061 caagccctca tagatgtgca aaggcatgta gcaatgatca tggtaatact gattgaacag
     9121 gttcagttcc tgctgcccgt aggtattgtt gttggtgtca tcacaatcca ggataatcac
     9181 tcccggttcc cgatcataag attcaacaaa agtatccacc agctcctgcc caatacgata
     9241 cagatcctgg cttttaatag agttctccag acggctcatc gtaggctggg aagcaagaga
     9301 caggcccgtt tgtggcaagc gacctgcaca ggtcttgaaa atcatgtcat ctcgaagatc
     9361 atcgcagtcg ttacaatctt cataacccgc agctatttga aaaactcgct gagtaagcat
     9421 ctctctgatc gtatgatcaa catatctttg atccctgtta tctgtaatac agttacttat
     9481 gcgattgatt aatcccatct ggttctcaac ttcccgcagt agcaacaacc cgccatcgct
     9541 gctgatatca taaccggtga agctgagttc aatttgtttg ttggatacag aaaaaagctc
     9601 gtgaactatt ggggtggtct cggaataatt ttttatcatt gtatgaagga gtgaaggttg
     9661 tcaatacctt gaatttactg atatttcagc aatttcaggg aactttctct cctttattta
     9721 tgaataattc agg
//
LOCUS       DAOPBK010000096         9676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6711_length_9676_cov_5.472924, whole genome shotgun sequence.
ACCESSION   DAOPBK010000096 DAOPBK010000000
VERSION     DAOPBK010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6711_length_9676_cov_5.472924"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1247)
                     /gene="recA"
                     /locus_tag="PLA17_06860"
     CDS             complement(237..1247)
                     /gene="recA"
                     /locus_tag="PLA17_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPK85171.1"
                     /translation="MEKKEINKEKLKALEVTLGKIEKDFGRGTIMKLGDHAIDNVQVI
                     STGSIGLDAALGIGGLPRGRVVEIYGPEASGKTTLAIHAIAEAQKAGGIAAIIDAEHA
                     FDRFYAEKLGVDVENLLISQPDNGEQALEITDHLIRSGAIDIIVIDSVAALTPKAEIE
                     GEMGDSKMGLQARLMSQALRKLTANINKTNTTCIFINQLREKIGLVFGNPETTTGGNA
                     LKFYASIRLDIRRTSQIKEGEDAVGNRVKVKVVKNKLAPPFKKAEFDIMFGEGISRTG
                     EIIDLGTEFNIIKKSGSWFSYGETKLAQGRDAVKRLLEDNPELAGEIEAKITEQLKEK
                     TK"
     gene            complement(1266..1733)
                     /gene="bcp"
                     /locus_tag="PLA17_06865"
     CDS             complement(1266..1733)
                     /gene="bcp"
                     /locus_tag="PLA17_06865"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPK85172.1"
                     /translation="MTQLTAGMPAPHFEGTDQNGNTVKLSDFLGKKVVLYFYPKDNTP
                     GCTAEACNLRDNYNDLLQKGFVVLGVSPDSEKSHLNFSGKYNLPFPLIADTEKKILTA
                     YGAYGEKMMYGKKVTGVIRTTFIIDEKGIIEKVIKKVNTKEHAAQIFKMYVNV"
     gene            1832..3004
                     /locus_tag="PLA17_06870"
     CDS             1832..3004
                     /locus_tag="PLA17_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85173.1"
                     /translation="MAIAIPVCLMLSLCPASAQQGRTKPSDGVVQAGKVRGNVAGVPD
                     EYFRMLGEAARKQHEADSASLLAGKAMLQAMSAGVDQKERYERKAVQDSTRAAARRAE
                     ADSLLLAVEKAAGRPPEKPVQDMKIAAHQLSRFDVLPGQAYTGSNPVPVEPVMPGGLV
                     YTIQIAVFKNIVNPSIFRGLTPVFGRKRQGGEALYYYVGLFRRLGDARNALPKARGAG
                     FPDAFIIALSDGTQISMEKASHLEKEWGSRPLDGDFAVTAGSKPAETATKPPAGASEP
                     VAQTPQVPIGTLSFRAEVMRIDKPVKPEVVQKIEALAGNRGLDMIKNSKGETVFLIGN
                     FITFESADEYVSLLIRNGYSSARVAAYVDRQEIPLEAARELVNWMPDDQKSTETGK"
     gene            2973..3242
                     /locus_tag="PLA17_06875"
     CDS             2973..3242
                     /locus_tag="PLA17_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HPK85174.1"
                     /translation="MTKRAPKPENKNRSEENLRSLMLINDDVNSFDHVIRSLVDVCGH
                     DEIQAEQCAVLTHIKGGCVIKIADVRTVEKMRMKLRELSLDTVVI"
     gene            3265..3729
                     /locus_tag="PLA17_06880"
     CDS             3265..3729
                     /locus_tag="PLA17_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPK85175.1"
                     /translation="MIIFLIFVGLLLFVIEFMLIPGVTIAGIGGAICLVTGIVMAFIS
                     FGTSTGMIVLGVTALVMVVTTVLMLRAKTWSKFMLKTAIDSKVDTVGSEEGKVKPGDR
                     GTTVTRLAPGGKVLVNGEYFEAKSVDLLIDPKQEIEVIRIEDNKLIVKPINN"
     gene            3732..4718
                     /gene="floA"
                     /locus_tag="PLA17_06885"
     CDS             3732..4718
                     /gene="floA"
                     /locus_tag="PLA17_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613657.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPK85176.1"
                     /translation="MSGIGIYVIIIIGAIILLWILLYFIPIGLWFQALVSGVRISLLQ
                     LIFMRWRKVPPPIIVRAMIEADKAGLKLNRDELEAHYLAGGHVAQVVHALVSANKANI
                     DLSFKMATAIDLAGRDVFNAVQMSVNPKVIDTPPVTAVARDGIQLIARARVTVRANIK
                     QLVGGAGEETVLARVGEGIVSSIGSSHSHKEVLENPDRISKTVLEKGLDAGTAFEILS
                     IDIADIDIGKNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKAQEARAKVI
                     EAEVEIPLAMAHAFKSGNLGIMDYYRMKNIEADTNMRDSISKPPTSGGEKKK"
     assembly_gap    4864..4963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4990..6264)
                     /locus_tag="PLA17_06890"
     CDS             complement(4990..6264)
                     /locus_tag="PLA17_06890"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HPK85177.1"
                     /translation="MKSFSVLPLLFLALSLQAQVAFDDYFTDRTMRFDFVMAGNSTTT
                     KVFPVSFREEPFWGGSLVNLIDPFNYGNFRYEIFDAVTGKLIYSRGFCTLYQEWQTTA
                     EAKQMERVFQEVATFPFPKNKVNFVLSIRGRDGQFSRLYETAIDPASYFITREKPEAS
                     LATRIAGSGDPHTSLDIAFIAEGYTSAEMEKFRNDVKRMAEYLFAEAPFDKYKDRINI
                     WAVEAPSQQSGTDVPGEGIYVNTALSSSYYTFDVDRYLTTQSIHRLNDYAASAPHDQI
                     IVLINSPRYGGGGVYNYYSAVTADHNLTPMVLTHEFGHGFAGLGDEYYSSEVAYEEFY
                     PLDVEPWEPNITTRVNFDAKWKDLIAKSVPIPTPNDKRYIGVTGLFEGGGYSARGIYR
                     PSYDCRMKSNVADGFCEVCQEALEKMILFYLE"
     gene            6354..7628
                     /locus_tag="PLA17_06895"
     CDS             6354..7628
                     /locus_tag="PLA17_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPK85178.1"
                     /translation="MFKSFDPVMAVRGLLASLGMPASAVSWLSTVILVAAVGLLAWLS
                     YNFTRVILVRVFTRIVRRSRSAYDDKFLETKMFKRLAMIVPGVIVYYLASWMLSANPG
                     WLVFAHKTAALYIVIYATLTLTAFIEGWHQVWLMQPISTGRSIKPYVQIVKVLVAFAG
                     ILIMISVLFRVSLTSVFAGLGVATAVLAVVFKDTLLGLVASVQLSTNNMVKLGDWITI
                     PGRNIDGTVIDLTLYTVKVENFDKTILTVPTYALISESFQNWKGMQDSGVRRIKREIR
                     IDVRSIVFVTPELLDSVSERMNVAKWLSENNEDIEELRNGGSTSLTNLGAFRAYMVEY
                     LKNHPHIDPMMTVMMRDMPPTDTGMPVEIYCFSKINTWVPFESVQSSVVDYAYAMVGQ
                     FGLKLFQSPSGEDLEALRSVTNQSVKKQNDKI"
     gene            7625..8845
                     /locus_tag="PLA17_06900"
     CDS             7625..8845
                     /locus_tag="PLA17_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPK85179.1"
                     /translation="MSKNYNEKLTAFIRAGIDDKPVTPRYDPFWQSLRSTGTELWLDT
                     GDMDEAEANWTAEMTALTTNNTLLNNEIQKGIYDSFVPQALELVSHLTINEQVKEIAF
                     ILNARHGLRLAKKFGGMVSVELHTDTAYDIDAIVSYGLRYRELCPEQFIVKVPYTPEG
                     LIGARLLHDMGVRINLTLEFSTRQNVLVTLIARPDYLNVFLGRIGAFIADNKLGDGSG
                     AGENALLASQSWVTRLSAETGSKTRQIAASLRNYTQLDLLAGADVYTIPPKVAAAGRK
                     SLGGLFESRLAQRYDVPLNDDAAGLGIEKFWEVPDSVVELGRQLGKEVPAYGEEIVAK
                     AHEAGVGDMFPVMDPEEKECIAAEGKIPVLSSWKERILSGNLSPDTLLTLAGLASFTG
                     DQKQLDDRVRGLIG"
     gene            complement(8922..>9676)
                     /gene="rlmB"
                     /locus_tag="PLA17_06905"
     CDS             complement(8922..>9676)
                     /gene="rlmB"
                     /locus_tag="PLA17_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPK85180.1"
                     /translation="SEKEFIFGMHPVMEAIRGGRQIDRVLVRQGLRGGQYHELMQVVN
                     EFAVPWQVVPQEKLDSITRKNHQGVIAWLSAIEFQDIENILPGIFEEGGEPLLLMLDG
                     VSDVRNFGAIVRSAHCFGAHAVIIPEKNSARISADAVKASAGALSTFQVCRVKSLTKC
                     LTYLKESGVKAVAATEKADSTVSAAALTGPLVVILGSEEKGISRELLGLSDAQVSIPI
                     TGSIGSLNVSVSAGIILYEVIRQRGARQQNLR"
BASE COUNT         2274 a         2284 c         2582 g         2436 t
ORIGIN      
        1 ggttagtaga ggataccggg agttggtttc ccatcggtta gtagaggata ccgggagttg
       61 gtttcccacc ggttagtaga ggataccggg agttgatttt ccgccaattg gtagaggatt
      121 gcaggagttg attttcctga ttatgaaaac aatccaaaag cctctgattt cttcaggggt
      181 tctgtggttc tgtcaggtac ctgacctgac cagcagcatc accgctgtct gatcagttat
      241 tttgttttct ctttgagctg ttcagtaatt tttgcctcaa tttccccggc cagttcgggg
      301 ttatcttcga ggagtctctt cacggcatcg cgtccctgtg caagctttgt ttcgccgtag
      361 gagaaccatg agccgctctt tttgattatg ttgaattccg ttccgaggtc aatgatttca
      421 ccggtacggg agatgccttc tccgaacatt atgtcaaact cggctttttt gaatggggga
      481 gccagcttgt tcttgacaac ctttaccttg acgcggttgc caacggcatc ttcaccctcc
      541 ttgatctggc tggtgcggcg gatgtcaagc cttatggagg cataaaattt aagcgcattt
      601 ccgccggtag tggtctcggg attgccaaac acgagcccta tcttttccct gagctggttt
      661 atgaagatac aggtggtgtt ggttttgttg atgttggcag tgagcttgcg cagggcctgt
      721 gacataagcc ttgcctggag ccccatcttc gagtcgccca tttctccttc aatctcagcc
      781 ttgggtgtga gggcggcaac cgagtcaata actatgatgt caattgctcc tgacctgatg
      841 aggtgatcag ttatctcgag agcctgttct ccattgtcag gctgtgagat gagcaggttc
      901 tccacatcca ctcccagttt ctcggcataa aacctgtcga aagcatgctc ggcatcaatg
      961 attgcggcta taccgcctgc cttttgcgcc tcggcaattg catggatggc cagggttgtt
     1021 ttccctgacg cctccggacc gtatatctcc acgacccttc cgcggggcag tccgccgatt
     1081 cccagcgctg catcgagacc aattgacccg gttgagataa cctgcacatt atcaatggca
     1141 tgatcgccca gcttcataat ggtgccccgg ccaaaatcct tttctatctt gcccagcgtc
     1201 acctcaagcg ccttcagctt ttccttgttg atttccttct tttccattca cttattcttt
     1261 agcagttaga cattaacgta cattttaaaa atctgggcgg cgtgctcctt tgtgttgacc
     1321 ttttttatca ccttctctat aattcccttt tcatcaataa taaaggtggt acgtatcaca
     1381 ccagtcacct tttttccata catcattttt tcgccgtagg cgccataggc agtcagtatc
     1441 ttcttttcag tatccgctat cagcgggaag gggaggttgt atttgcctga aaaattcagg
     1501 tgtgattttt cactgtcagg actgactccc agcacgacga atcccttctg aagaagatca
     1561 ttgtaattgt ctctaagatt gcatgcctct gctgtacacc cgggtgtatt gtctttggga
     1621 taaaaataga gtactacttt tttgccgagg aagtcactga gtttcactgt atttccgttc
     1681 tggtctgttc cctcgaagtg cggtgccggc atgcctgcgg tgagctgagt catgactttt
     1741 tgttattttt gttataagtg gtaaaggtat gaagttatca tctttcacaa aagatttgca
     1801 cccgcgagtt attaacagag gggtgatgcg ggtggcaatt gcgataccgg tctgtctgat
     1861 gctgtcacta tgccctgcat ctgctcagca gggccgcacg aagccgtcag atggtgttgt
     1921 acaggcaggg aaggtgcggg gaaatgtggc cggggtgccg gacgaatact tcaggatgct
     1981 tggtgaagct gcccggaaac agcatgaggc tgattcagct tcacttctgg ccgggaaggc
     2041 tatgctgcag gccatgagcg ccggcgttga tcagaaggag aggtatgaaa ggaaagctgt
     2101 tcaggacagc acccgggctg ctgcccgaag agcagaggct gactcactgt tgcttgcagt
     2161 ggaaaaggct gcgggacgtc ctcctgaaaa gccagtgcag gacatgaaga tcgcggctca
     2221 tcaactcagc cggtttgatg ttttgcccgg acaggcttac accgggagca atccggtgcc
     2281 tgtagagccg gtcatgccgg gaggactggt ctataccatc cagatagccg tattcaagaa
     2341 tattgttaat ccgtcgatct tcaggggact cactcctgtt tttggaagga agaggcaggg
     2401 gggcgaagca ctctattatt atgtgggact gtttcgcagg cttggtgatg ccaggaatgc
     2461 tcttcctaaa gcaaggggag caggtttccc cgatgcattt atcatagcgc tttctgacgg
     2521 cacacagatc tccatggaga aggcttccca ccttgaaaag gagtggggct cacgtccgct
     2581 ggatggcgat tttgccgtga ccgccggctc aaagcctgcc gaaacagcca caaagccccc
     2641 ggccggtgcc tcagaacctg tggcacaaac tccacaggtg ccaatcggta cactctcatt
     2701 cagggctgag gtgatgcgca tcgacaagcc tgtcaagcct gaagtggtgc agaagatcga
     2761 ggcgctggca ggcaaccgcg gtcttgacat gatcaaaaac agcaaaggtg aaaccgtgtt
     2821 tctcattggt aattttatta catttgaaag cgctgatgag tatgtctcgc tgctgatacg
     2881 caatggctac agttccgcaa gggttgcagc ctatgtagac agacaggaga ttcctctgga
     2941 agcagcaagg gaattggtaa actggatgcc ggatgaccaa aagagcaccg aaaccggaaa
     3001 ataaaaacag gagcgaggag aatctgagat ctctcatgct tatcaatgat gatgtcaaca
     3061 gctttgatca tgtcatcaga tcacttgttg atgtctgcgg tcatgatgag atccaggcag
     3121 aacagtgcgc tgtgcttacc catatcaaag gcggttgtgt catcaagata gctgatgtcc
     3181 gaacagttga gaaaatgaga atgaagctca gggaactgag ccttgataca gtagttatat
     3241 aatgcattca acatgtctct gggattgatc atatttctga tattcgtagg cctcctgctc
     3301 ttcgtcatag agtttatgct tattccagga gtaacaattg cgggcatagg cggcgcaatc
     3361 tgtcttgtga cagggatcgt aatggccttt atttcgtttg gcacttcaac aggaatgata
     3421 gtactcggag taactgcact tgtgatggtt gtcacaactg ttttgatgct gagggccaaa
     3481 acctggagca agtttatgct caagacagcc attgacagca aggttgatac cgttggctct
     3541 gaagagggaa aggtaaagcc cggtgacagg ggtacaacag tcaccaggct ggctccgggc
     3601 ggcaaggttc tggtcaacgg agagtatttc gaggcaaaat ctgttgacct gctcattgac
     3661 ccgaagcagg agatagaagt catcagaata gaagacaaca aactgattgt aaaaccaata
     3721 aataattgat tatgagcgga ataggcattt atgtaataat catcatcggg gcaataatcc
     3781 tgctctggat tcttctttac tttatcccca tcgggctctg gtttcaggct cttgtctcag
     3841 gtgtgagaat aagccttctt cagttgattt tcatgcgctg gaggaaagtc ccgccgccaa
     3901 tcatcgtcag agcaatgatc gaggctgaca aggccggatt gaagctgaac agggatgagt
     3961 tggaggccca ctatcttgca ggcggacatg tagcccaggt ggtgcatgca cttgtctcgg
     4021 ccaataaggc gaacatagac ctgtcgttca agatggcaac agctattgac cttgcaggcc
     4081 gtgatgtttt taatgcggta cagatgtcag ttaacccgaa ggtgattgac actcctcccg
     4141 tgacagccgt tgcccgtgac ggtattcagc ttatcgccag ggcgagggtt accgtaagag
     4201 ccaatatcaa gcagcttgtc ggtggagcag gggaggagac ggtgcttgcc cgcgtcggcg
     4261 agggtatcgt gtcatcgatc ggttcgtcgc acagccataa ggaagtgctt gagaatcctg
     4321 acaggataag caagacggtg cttgagaagg gtcttgatgc aggaacagct tttgagattc
     4381 tctcgattga tatcgcggac attgatatag gcaagaacat cggtgccgtg ctgcagatgg
     4441 accaggccaa tgctgacaag aacattgccc aggccaaggc tgaggagcgt cgtgcgatgg
     4501 cagtggccct cgaacaggag atgaaagcga aagctcagga agcacgtgcc aaggtcatcg
     4561 aggctgaggt tgaaattcct ctggcaatgg ctcatgcttt caagagcggc aacctgggta
     4621 tcatggatta ctacaggatg aagaacatcg aggctgacac caacatgcgt gattccatct
     4681 ccaaaccgcc aacttcggga ggagagaaga agaaatagtc acctcaataa ttccctttgg
     4741 ggatactact gctgcagtta agcaatgact gacagcggtt atggtctttt atcctatcca
     4801 gcgcggaatt ccgggatacg actgattttg atgctgcggg aaacgactac tgctgctgag
     4861 cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngctgaca ggcaatagcc
     4981 ggctttaagc tactccaggt agaaaagtat cattttctca agcgcctcct ggcacacttc
     5041 acagaacccg tctgccacgt tgctcttcat gcggcagtca tatgagggcc tgtaaatgcc
     5101 tctggcggag tagccccctc cctcaaagag gccggtaaca ccgatatacc tcttatcatt
     5161 tggcgttggt atgggtactg acttggctat cagatccttc catttggcat caaaattcac
     5221 ccttgtggta atatttggtt cccagggttc cacatcgaga ggataaaact cttcatatgc
     5281 cacctctgag gagtaatact catctcccag tccggcaaat ccgtgaccga actcgtgcgt
     5341 cagcaccatt ggggtaagat tgtgatcagc cgtgacggcg ctgtagtagt tatacactcc
     5401 gccaccgccg tatctggggc tgttgatgag cactatgatc tggtcatgcg gggctgaagc
     5461 ggcgtagtca ttgagcctgt gtatactctg tgtggtcagg tagcggtcaa catcgaaggt
     5521 gtagtagctt gagctgagag cagtattgac atagattcct tctcccggca catccgttcc
     5581 ggattgctga gagggagctt caacggccca gatatttatg cggtctttgt atttgtcaaa
     5641 gggtgcttca gcgaagaggt actccgccat ccgtttgaca tcgttccgga acttttccat
     5701 ctcagcgctg gtgtaacctt cggcaatgaa tgcgatatca agtgaggtat ggggatcgcc
     5761 gctgccggct attcgggttg ccagggaggc ttcaggtttc tccctggtaa tgaaatagct
     5821 ggcagggtca attgccgtct cgtacagtct ggagaactgg ccgtcgcgtc ccctgatgct
     5881 cagaacgaaa ttgaccttgt ttttcggaaa agggaaggtt gcaacttcct ggaaaacgcg
     5941 ttccatctgc ttcgcttcgg cggtggtctg ccactcctgg tatagggtgc agaatccgcg
     6001 cgagtaaatc agtttcccgg taacggcatc aaaaatctcg tacctgaaat tgccgtaatt
     6061 gaacggatca atcaggttga ccagtgagcc tccccagaag ggttcctccc tgaaggagac
     6121 aggaaatact ttggttgttg tgctgttgcc ggccattacg aaatcaaacc tcatggtgcg
     6181 gtcagtgaaa taatcatcga atgcaacctg ggcctgaagt gaaagtgcca ggaacaaaag
     6241 cggtaatact gagaaagact tcatgatttg gcagttttac tgcgtaaagg tagtaaacag
     6301 gctgccacaa aaaaagataa ttagtaaatt cacccgcgta aaaaaagttg ataatgttca
     6361 aatcattcga tccggtaatg gcagtcaggg ggctcctcgc gagtctgggg atgccggctt
     6421 cagccgtttc atggctgagt acggtcatcc tggtggctgc agtgggcctc ctggcctggc
     6481 tgtcatataa ttttacccgt gtcatcctcg tcagggtatt tacccgcatc gtaaggcgtt
     6541 cgcggtcagc gtatgacgac aagttcctgg agacgaagat gttcaagcgc ctggcaatga
     6601 tagtgcccgg agtcattgtc tattatctgg caagctggat gcttagcgcg aatcccggct
     6661 ggctggtatt tgcccataag accgctgcgc tctatatagt gatttacgct accctgacac
     6721 tgaccgcatt catcgaggga tggcaccagg tctggctgat gcagcccatt tctacgggga
     6781 gatcaatcaa accttacgtg cagatagtca aggtgctggt ggcatttgcc ggaattctga
     6841 tcatgatttc cgttctcttc agggtgtctc ttacaagtgt ttttgcagga ctgggcgtgg
     6901 caacagccgt actggcagtg gtattcaagg atacactgct gggccttgtg gccagcgtac
     6961 agctctcaac caacaatatg gtgaagctgg gcgactggat aacgatcccc ggaaggaaca
     7021 tcgacggtac ggttattgac ctgacactct atacggttaa ggtcgaaaac tttgacaaga
     7081 cgatactcac ggtgcctacc tatgccctga tatcagaatc ttttcagaac tggaagggga
     7141 tgcaggattc aggggtaaga aggatcaaac gggaaatccg cattgatgta cgttcaattg
     7201 tctttgtgac gcctgaactg ctggactccg tctctgagag gatgaacgtg gcgaagtggc
     7261 tctcagaaaa caatgaagat atagaggaac tcaggaatgg cgggtccacc tcacttacga
     7321 acctcggggc attcagggct tatatggttg aatatctcaa aaatcatcct catattgatc
     7381 ccatgatgac tgtgatgatg cgtgacatgc ctcccacgga cacaggcatg ccggtggaga
     7441 tatactgttt cagcaagatt aatacgtggg tgccgtttga gagtgttcag tcatcagtgg
     7501 tcgattatgc atatgccatg gtgggccagt ttgggctgaa actcttccag agtccctccg
     7561 gggaagacct tgaggctctc aggagcgtga caaatcaaag tgtaaaaaaa caaaatgata
     7621 agatatgagt aagaactata atgaaaagct gaccgccttt atcagggccg gtatcgatga
     7681 caaacctgtt actccacgat atgatccctt ctggcagtca ttgcgctcaa cgggaacaga
     7741 gctctggctt gacaccggtg acatggatga agctgaggct aactggactg ccgagatgac
     7801 cgcacttaca acaaacaata cgcttctgaa caacgagatt cagaagggga tatatgattc
     7861 gttcgtgccg caggcgctgg aactggtcag tcatcttacc atcaatgagc aggtgaaaga
     7921 gatagccttt attctgaatg cccgtcacgg tcttcgtctc gcgaagaagt tcggtgggat
     7981 ggtaagtgtt gaactgcata ccgatacggc atatgacatt gatgccatcg tttcatacgg
     8041 actgcgctac agggagctct gtccggagca gttcatcgtt aaggttccct atacccctga
     8101 aggacttatt ggtgcccggt tattgcatga catgggagtg cggatcaact taacccttga
     8161 gttcagcacc cgccagaatg tgctggtgac cctgatagcc aggcctgact acctgaatgt
     8221 gttcctcgga aggatagggg cattcattgc tgacaataag ctgggcgacg gcagcggtgc
     8281 aggagagaac gctttgcttg catcacagtc atgggttacg cggttatctg ctgagacagg
     8341 ctcgaagacc aggcagatag ctgccagcct caggaattac actcagcttg acctgctggc
     8401 aggagccgat gtctatacca tcccgccgaa ggtggcagct gccggcagga aatcactcgg
     8461 cggtctcttc gagagcaggc ttgcacagcg ctatgatgtg cctctgaacg atgatgcggc
     8521 aggactggga attgaaaaat tctgggaggt gccggattcg gttgttgagc tgggcagaca
     8581 gcttgggaag gaggttccgg cttatggtga ggagattgtt gcaaaggcgc acgaggcagg
     8641 agtgggtgac atgttcccgg tgatggatcc tgaggagaag gaatgcattg ctgcggaagg
     8701 aaagataccg gttctttctt catggaagga acggattctc tcaggaaacc tctcccccga
     8761 caccctgctg acccttgccg gcctggcctc attcactggt gaccagaagc agttggatga
     8821 ccgggtaaga ggactgatag gctgatagaa ttgcaggact gtcaatacgt ggtatctgaa
     8881 ttcttcacca tggtaaaggt attgacaggc gttcataaga actatctcag gttttgctgc
     8941 ctggctcccc tctgccttat aacctcatat aggatgattc ctgctgaaac ggatacatta
     9001 agtgatccga tggagcctgt gatgggtatg gaaacctgtg cgtcagacaa ccccagcagt
     9061 tcgcggctga tccccttctc ttccgagccg agaatcacaa ccagtggtcc ggtcagtgcg
     9121 gcagcgctta ccgtgctgtc agccttctca gtggcagcca cggctttcac tcctgactcc
     9181 ttcaggtagg tcaggcactt tgtcaggctc ttcacccggc atacctggaa agtgctgagg
     9241 gccccggctg atgccttgac cgcatcggca gatatccttg cggaattttt ttcagggatg
     9301 atcaccgcgt gtgctccgaa acagtgtgct gaccgcacga tggctccgaa gttgcgcacg
     9361 tcactgacgc cgtcgagcat cagcaggagc ggttcgccgc cctcttcaaa aataccgggc
     9421 aggatattct caatatcctg gaattctatt gcagagagcc atgctattac cccctgatgg
     9481 ttcttgcggg ttatggaatc aagcttctcc tgcggcacca cctgccatgg cacggcaaac
     9541 tcgttcacca cctgcatcag ctcatggtac tgtccgccgc ggagtccctg gcggaccagc
     9601 acacggtcta tctgcctgcc tcccctgatg gcttccatga cagggtgcat cccgaaaatg
     9661 aattcctttt cgctgt
//
LOCUS       DAOPBK010000097         9529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6825_length_9529_cov_4.490395, whole genome shotgun sequence.
ACCESSION   DAOPBK010000097 DAOPBK010000000
VERSION     DAOPBK010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6825_length_9529_cov_4.490395"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLA17_06910"
     CDS             <1..618
                     /locus_tag="PLA17_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85181.1"
                     /translation="SLSYSFLDSVIRLYDDPVVWSRAYQMTADSIALFTVNSKADRMD
                     MYNSAFVVSEIDTSRYDQISGKNLSGYFTDNKLTRIKVTGNGEAIYYVVDGEELVGVN
                     RARSATIEILFEESRIVEIIQNQSPDGVLNPPLEIPDSEMRLDGYRWHPDLRPDRKGM
                     GIDELFPPPPEGALEEVRERAPEEEAGPEKAKVPGTAVEMTVPGT"
     gene            complement(639..860)
                     /locus_tag="PLA17_06915"
     CDS             complement(639..860)
                     /locus_tag="PLA17_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPK85182.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRSGAPMEVIENLQEIE
                     DEGDIYESIEDIWPDYPSKDDFFFNEDEY"
     gene            complement(957..1505)
                     /locus_tag="PLA17_06920"
     CDS             complement(957..1505)
                     /locus_tag="PLA17_06920"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPK85183.1"
                     /translation="MKIYTKTGDKGETSVIGGVRLPKHHLRIEAYGTLDELIAWIGML
                     RSCEHSCIPHEELIEIQSVLMSCCTVIATHPSAKVPEGIHIGDTSRLESSIDRMESDL
                     PGLKSFILPGGNNPAATANLARTVCRRAERAILRLNETEKTDPDVIRYLNRLSDYLFV
                     LSRHLMHSIGTEEVNRISDKKA"
     gene            complement(1577..2287)
                     /locus_tag="PLA17_06925"
     CDS             complement(1577..2287)
                     /locus_tag="PLA17_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK85184.1"
                     /translation="MDKIIEIKGIVKNYQVGTVIVRALRSVSLDISRNEYVAIMGPSG
                     SGKSTLMNILGCLDTPTKGEYILNGTDVSKMADDRLAEIRNKEIGFVFQTFNLLPRYT
                     ALENVTLPLIYAGVPRHEREDLAVKTLDLVGLGDRMTHKPNELSGGQRQRVAIARALI
                     NNPSIILADEPTGNLDSKTSRDIMALLDKIHEDGNTVIVVTHEEDIARHAHRIIKLMD
                     GEVSADYINENRIEEIKK"
     assembly_gap    2455..2554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2555..3241)
                     /locus_tag="PLA17_06930"
     CDS             complement(<2555..3241)
                     /locus_tag="PLA17_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85185.1"
                     /translation="MSLPLEFNELSEEMNLHAASAYALHLLTARSTAGHGVHSPWMFR
                     FITEVIGGRGDSAIMRGCSPRALERSAGGRSDNAIMRGCNPRAMERSARGRSDNAIMR
                     SCYPSALQGSARGRGDSAIMREVESLRQEMLSDRRMVMVTDLGAGSSVMKSRERSVRH
                     IASAAAVPKREAALLARIAESLNNTRAGEPGDADRHAEQSGTGLKSGKMAATDPVPAV
                     VPDSGHSCREV"
     gene            3476..5581
                     /gene="recG"
                     /locus_tag="PLA17_06935"
     CDS             3476..5581
                     /gene="recG"
                     /locus_tag="PLA17_06935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPK85186.1"
                     /translation="MSELLDSEIKFLPGVGPKRAELLGAEADIHTFRDLIYYFPYRYI
                     DKTRFYKVRELDTDMQYVQVKGKILRFTAEGAGKGRRLTADFCDETGIVKLVWFKGHK
                     WVLNSYKPGKEYIIFGKPTIFSGAFNIVHPEIEEAGKRETGVSSALEAQYNTSEKMKS
                     HFLHSKAIRKLMSKAFSMPGLRFPETLPSSVTEPMKLMSLSDALFRIHFPDTNNDIDN
                     ARFRLKFEELFYIQLNLLRYKNNRTKSTHGFLFPVVGERLNSFYANNLPFELTGAQKR
                     VVREIRADLGSGRQMNRLLQGDVGSGKTMVALMSMLIAADNGFQSCLMAPTEVLAIQH
                     FNSISKFVKGIDAKVALLTGSTKKSERTRLEQGLLDGSIDILIGTHALIEENVQYKNL
                     GLVIIDEQHRFGVAQRAKLWQKSDYPPHVLVMTATPIPRTLAMTLYGDLDVSVIDEMP
                     PGRKPVKTMTFTDAEREKVFGFMRRQIEDGRQIYVVYPLIKESESLSYKDLEDGLESI
                     ARAFPPPQYCISVVHGRMSPKDKEISMNLFRRGQAHIMVATTVIEVGVDVPNASVMVI
                     ESAERFGLSQLHQLRGRVGRGADQSYCILMTSHKLSADAAERVNTMIATTDGFEIAEA
                     DLRMRGPGDLEGTQQSGMAFDLKISDLGKDGQILNLARSRATEILSDDPELSNPANRI
                     LKVNLKRLFGKKYSWSEIS"
     gene            6131..6547
                     /locus_tag="PLA17_06940"
     CDS             6131..6547
                     /locus_tag="PLA17_06940"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPK85187.1"
                     /translation="MPRGETIAHEGKVLSVPGDGTAEVEILVAGACSGCHAKSVCSAG
                     KSESRIITAKCDVPLHPGDNVRVEMKLSQGFGALLIGYIIPFIVLVTAFVAALLAGAG
                     ELASALISFASIGCWYLAVWLLRGRIGEKFEFKIKV"
     gene            6551..7414
                     /locus_tag="PLA17_06945"
     CDS             6551..7414
                     /locus_tag="PLA17_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPK85188.1"
                     /translation="MSSTVLLTIVMLSTLGLLAAIILYFVAQKFRVVEDPRIDTVTEA
                     LPGANCGGCGYTGCRAFAETLVNSTSFEGLNCPVGGAETMGKIAVILDRQSVAVEPKV
                     AVMRCNGTPDNATRTSYYDGAVSCRVAHSLYGGQTGCQYGCLGCGDCVTACKFDAIYL
                     DPVTQLPVILDEKCVACGACVKACPRSIIELRKRNKKDRKIYVSCVNRDKGGPARKAC
                     TAACIGCGKCVKVCSYEAITIENNVAYIDSFKCRLCRKCVQECPTGAILEIGFPVKEA
                     KPAEPADAAVN"
     gene            7508..8842
                     /gene="rsxC"
                     /locus_tag="PLA17_06950"
     CDS             7508..8842
                     /gene="rsxC"
                     /locus_tag="PLA17_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPK85189.1"
                     /translation="MLKTFPKGGIHPPENKLTADRPVLILPVPSQVTVPVSQHIGAPA
                     VPVVEKGTAVKTGQVIAQAKGFVSTNIHSPVSGKVNRIDTVVDTTGYKQTAVVIDVEG
                     DEWIDSIDRSATLVKEITLSREQIIERCQQCGLVGMGGATFPTHIKLTVPAGKKCDIL
                     IINGVECEPYLTSDHRLMLEKGAEVLTGASIIMKALGVENAVIGIEANKPDAICHLTE
                     LAMDYKGITVQPLKVKYPQGGEKQLIKACTGREVPSGGLPVDVGVVVQNVGTSFAVYE
                     AVQKNKPLIERVVTVTGMSVKNPGNFLVRIGTPVTALIEATGGMPDDTGKVVSGGPMM
                     GKALAMTDVPVTKGTSGIIMFPADVSRRAEVMPCIRCGKCSSVCAMGLEPWLISVLAQ
                     KAMYEKAEQEKITDCMECGACAYTCPAKRPLLDYIRLGKATVIRMARERVTK"
     gene            8984..>9529
                     /locus_tag="PLA17_06955"
     CDS             8984..>9529
                     /locus_tag="PLA17_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPK85190.1"
                     /translation="MSNILNVSPSPHQQSAETTRKLMMGVVIALAPALAASIWYFGLG
                     AIIVTLVSVVSCVAVEYLIQKFILKTKPSVSDGSAIVTGLLLAFNVPSNLPVHIIIIG
                     AIVSIGIAKMTFGGLGNNPFNPALVGRVFLLISFPVQMTSWPVPSGFNTSYTDAVTGA
                     TPLAIMSEGIRNGEAVSTLMEQ"
BASE COUNT         2252 a         2286 c         2512 g         2379 t
ORIGIN      
        1 tccctttcct actcatttct cgactcggtc atcaggttat atgatgatcc cgttgtatgg
       61 tcaagggctt accagatgac ggcagattcc atagccctgt ttacagtcaa cagcaaggct
      121 gaccgtatgg atatgtacaa cagtgcattt gtggtcagtg agattgacac gtcacgttat
      181 gaccagatca gcgggaagaa tctttcaggc tatttcactg ataacaagct gaccaggata
      241 aaggtcaccg ggaatggtga ggctatttac tatgtagttg atggagagga gcttgttggt
      301 gtcaaccggg cgcgtagcgc caccattgag atactgtttg aggagagcag gattgtggag
      361 ataatccaga accagagtcc tgacggagtt ctcaatcctc ccctggagat acctgactcc
      421 gagatgcggc ttgacggata caggtggcat cctgatctcc ggcctgaccg taaagggatg
      481 ggtattgacg agttgttccc tccgcctccg gagggtgctc tggaagaggt ccgggagcgt
      541 gccccggagg aggaagcggg gcctgaaaaa gccaaagtgc ccggaactgc cgtcgaaatg
      601 accgttccag gcacttagtg tgcttatagc aacagctgct agtactcgtc ttcgttgaag
      661 aagaagtcgt cctttgaggg gtagtcaggc cagatatctt caatactttc gtagatatca
      721 ccctcatctt ctatttcctg aaggttttcg attacctcca tgggggcacc tgacctgatc
      781 gcatagtcaa taagctcgtc ttttgtggcg ggccaaggcg cgtcttccag ttttgatgct
      841 aattcaagtg tccagtacat aatcaagaaa attattttat tacccttctc taagagtccg
      901 caaatataaa attatttaga ataaaaaaag acccggacag gtttttacac ggattattat
      961 gcttttttat cagaaattcg attaacttct tctgttccaa tactatgcat aagatgtctg
     1021 gataagacga agaggtagtc tgacagccgg ttgaggtatc ttatgacgtc agggtcagtt
     1081 ttctcggttt cattgagccg gaggattgcc cgttcggcac ggcggcagac ggtacgggca
     1141 aggttggctg ttgcagcggg attatttcct ccgggaagga tgaatgattt caatccgggc
     1201 aggtcactct ccatccggtc tattgaactt tccagccggc tggtatcccc gatgtggatt
     1261 ccttcaggaa ctttggcaga tgggtgggtg gcgataactg tgcagcatga catgagtacc
     1321 gactggattt caatcagttc ttcatgtgga atgcaggagt gttcgcaaga gcggagcata
     1381 cctatccatg caataagttc atcgagggta ccgtaggctt cgatacggag atgatgtttc
     1441 ggtagtctga cacctccgat aactgatgtc tcgcctttat cgccggtttt ggtatatatt
     1501 ttcatcgcct ttacctgacc ggaactggta tggttatcaa gacccggtca ttctgcatcg
     1561 ggttatccgt tgcaatttac tttttaattt cttcgattcg gttttcattg atatagtcag
     1621 ccgagacctc accatccatc agtttgatga ttctgtgtgc atgacgtgcg atatcttcct
     1681 cgtgcgtaac tacgataact gtattgccgt cttcatggat cttgtcaagc agggccatga
     1741 tgtctctgga ggttttgctg tcgaggttac cggttggttc gtcggcgagg attattgagg
     1801 ggttgttgat cagagccctg gcgattgcca ctctctggcg ttgtcctcct gaaagttcat
     1861 taggtttatg ggtcattctg tcgcccagtc ccacaaggtc aagtgtcttg acagcaaggt
     1921 cttcgcgttc atggcggggt actccggcgt atatgagcgg gagggtgacg ttttccagtg
     1981 cggtatatct gggcagcagg ttaaatgtct gaaagacaaa gcctatctcc ttattgcgga
     2041 tttccgcgag cctgtcgtct gccatcttgc tgacgtcagt tccgttaagg atatactctc
     2101 ctttagtggg tgtatcgagg catccgagga tattcatcag tgttgacttt ccggatccgg
     2161 aagggcccat aatggccacg tattcatttc tgctgatatc gagcgaaact gaacggagag
     2221 ctctgacgat gactgttcct acctgataat ttttgactat tcccttaatc tcaatgattt
     2281 tatccatgcc tcttttatta aagttccgaa attagttcaa attaagatat aatactgttt
     2341 cgtcgctgtt attttttgtt aataccgcac cctgtaatgc cccggtgtga tggatggaat
     2401 acagaacagc attcccatac gcagtgtttc gagggtagcc tgtattgggc cgggnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaacctc ccggcaagaa tggccggagt
     2581 caggtactac agcgggtacc ggatcggttg cagccatttt cccggattta aggccggttc
     2641 cggattgctc cgcatgccgg tctgcgtctc ccggttcacc tgccctggta ttattcaggc
     2701 tttcggctat cctggccagc agcgctgcct ctcttttcgg cacagcggca gcagaggcga
     2761 tatgccgtac acttcgctcc ctgctcttca taaccgatga tcctgcgcca agatcggtca
     2821 ccatcaccat ccgcctgtcg gaaagcatct cctgcctgag gctctcaacc tcccgcatta
     2881 ttgcactgtc acccctgccc ctagcacttc cctgcaaagc gctggggtaa caactcctca
     2941 ttattgcgtt gtcactcctg ccccttgcac ttctctccat agcgcggggg ttacatcctc
     3001 tcattattgc gttatcactc ctgccccctg cacttctctc caatgcacgg gggctacatc
     3061 cccgcattat tgcactgtca cccctgcccc ctatcacctc tgtgatgaac ctgaacatcc
     3121 agggactgtg cactccgtgg cctgcagtgc ttcgggcagt cagcaggtgc aatgcataag
     3181 ccgatgctgc atggaggttc atttcttcag acagttcgtt aaactccaga ggtagtgaca
     3241 cagtggtggt attgacgtta tcaggttcat ttcttcagac agttcttaaa ttccagaggg
     3301 agtgacacag tggtggtatt gacgttatca ggttcatttc ttcagacagt tcttaaattc
     3361 cagagggagt catagttgtg gcattggcgt catcaggttc atttcttcag acagttaatt
     3421 tgattcccgg tcaaaattag ctaattttcg gtgacattcc tttttaccac cagtgatgtc
     3481 tgaattgctt gacagtgaga taaaattcct tcccggagtg ggccccaaac gggccgaact
     3541 gcttggggcg gaggcagaca tacacacatt ccgtgacctg atctattact tcccttacag
     3601 gtacatcgat aaaacccgtt tctacaaggt cagggagctt gacaccgaca tgcagtacgt
     3661 gcaggttaag ggtaagattc tcagattcac cgccgagggg gccggaaaag gcagaaggct
     3721 tacagcagac ttctgcgatg agaccggcat cgtaaagctt gtctggttca aaggacacaa
     3781 atgggttctc aactcataca aacccgggaa ggagtacatc atcttcggca agcccaccat
     3841 cttcagcggt gcattcaaca ttgtacatcc ggaaattgaa gaggccggga agcgtgaaac
     3901 aggtgtttcc tctgcacttg aagcacagta caacacctcg gagaagatga agagtcactt
     3961 cttgcattca aaggcaatca ggaagctcat gtcaaaggca ttttcaatgc cggggctccg
     4021 tttccctgag actctcccct cgtcggtcac tgaaccgatg aagctgatga gcctctctga
     4081 tgcccttttc agaatccact tccccgatac gaacaatgac atagacaatg cccgtttcag
     4141 gctgaaattc gaggaactgt tctacatcca gcttaacctg ctgagataca agaataaccg
     4201 aacgaagagc acacacgggt tcctcttccc ggtagtaggt gaaaggctca attcatttta
     4261 cgccaacaac cttccctttg aactcaccgg ggcacagaag cgggtggtgc gtgagatccg
     4321 tgccgatctg ggcagcggca ggcagatgaa ccggctcctt cagggtgatg tgggcagcgg
     4381 aaaaacgatg gttgcgctta tgagcatgct gatagctgcc gacaacggat ttcagagctg
     4441 tctgatggcc cccacggaag ttcttgccat tcaacacttt aactccataa gcaaattcgt
     4501 caaaggaatc gatgcaaagg tggcgctcct gacaggatcg acaaagaaaa gtgagcggac
     4561 aaggctggaa caggggctgc ttgacggttc aattgacata ttgatcggga cacacgcgct
     4621 gattgaggaa aacgtgcagt ataagaatct gggtctggtt attattgacg agcagcatcg
     4681 tttcggtgtc gcgcagcgcg cgaagctatg gcagaagagt gattatccgc cgcatgtgct
     4741 tgtgatgact gccacgccta ttccccgcac gcttgccatg accctttacg gtgacctcga
     4801 tgtctctgtg attgacgaga tgccgccagg gcgaaagcct gtaaagacaa tgacattcac
     4861 cgatgccgag cgcgaaaagg ttttcgggtt catgcggagg cagattgagg acgggcgtca
     4921 gatttacgtt gtttatccgc tgataaagga gtcggaatca ctctcttaca aggatctcga
     4981 agatggtctg gagagcatag cccgtgcctt cccgccgccg cagtactgca tcagtgttgt
     5041 tcacgggcgg atgagtccga aggacaagga gatctcgatg aatttgtttc gcaggggtca
     5101 ggcgcatatt atggttgcaa caaccgtgat tgaggtgggt gttgacgtgc ccaatgccag
     5161 tgtgatggtt attgagagcg cggagcgttt cggcctctcg cagctgcatc agttgcgcgg
     5221 gagggtgggc cgtggcgcag accagtcgta ttgcatactg atgacctcgc acaagctctc
     5281 agcagatgct gcggagcgcg tcaatacgat gattgccaca actgacggtt ttgagatcgc
     5341 ggaagctgat ctgcgaatgc gggggccggg tgacctggag gggacgcagc agagcggaat
     5401 ggcatttgac ctgaagatat ctgacctggg gaaggacggg cagatactca acctggcgcg
     5461 atcgcgtgcc acggagatcc tgtctgatga cccggaactg tcaaatccgg cgaacagaat
     5521 cctgaaagtc aacctgaaaa ggttattcgg aaagaagtac tcgtggagtg agatcagctg
     5581 agcaggcctg ccacactcac ccgggcacaa agagagcatc ccctggtgta gagtgagatt
     5641 agttaagcac gttattacac tcaccagata catggagaga gcatcccctg ccatgaagtg
     5701 agatcagctg agcaggagtg tacaatgctt tatgcacaac acgcacaata attggcagaa
     5761 ggccatcaga aattgcagaa cagaaacagg agaacaaaac tcccctcaga tgattcagca
     5821 ttgtattccg taaagaatca gacctttata ccctcagact catagaccct gtgaccctgt
     5881 ggccttaaga cccatagatt ccaggtcctc aaacattcag actcatcaac aatagttatc
     5941 tatggtttcc aaatgactac tcataactat ctgattatca gaactgatta tctttcctaa
     6001 aatgagtgat cttattaatt gattatgtgt ctattagcag aaacttacca gcctgttaat
     6061 taaaataatg accaatatta tatctttttt cccggtaatt ccgtaaaatt gccatttcaa
     6121 ttatccattg atgccacggg gagaaaccat tgctcatgag gggaaggtat tgagtgttcc
     6181 cggcgacggc acagccgagg ttgagattct tgttgccggg gcctgttcgg gttgtcatgc
     6241 aaagtcagta tgttctgccg gaaaatcaga gagcagaatc attactgcga agtgtgacgt
     6301 gcccctccat cccggtgaca atgtcagggt tgagatgaag ctctcgcagg gtttcggggc
     6361 gcttctcata ggttacatta ttcccttcat cgtgcttgtc actgcatttg tagctgctct
     6421 gctggccggt gcgggtgaac tggcttccgc cctgatctct tttgcatcta ttggatgctg
     6481 gtacctggct gtatggttgc tgagaggccg cattggtgaa aaatttgaat tcaaaataaa
     6541 ggtctgactt atgagttcaa ccgttctatt gactatcgtg atgctgagca ccctgggcct
     6601 gcttgccgcg attattctct attttgtggc gcagaaattc agggtggttg aagatccccg
     6661 catcgatacg gttacagagg cacttcccgg tgccaactgt ggtggatgcg gttataccgg
     6721 gtgtcgcgca ttcgcggaaa cactggtaaa ttcaacatct ttcgaaggac ttaactgccc
     6781 tgtcggcgga gccgaaacaa tgggcaaaat agctgttatc cttgaccgtc agtctgttgc
     6841 ggtggagcca aaagtggctg tcatgaggtg caacggtact cctgacaacg cgacccggac
     6901 gtcgtactat gatggtgctg tcagttgccg tgttgcgcat tcactatatg gtggacagac
     6961 aggctgccag tatgggtgcc tgggatgcgg cgactgtgtg acggcatgca agtttgatgc
     7021 gatatacctc gatcccgtta ctcagctgcc ggttattctc gatgagaagt gtgttgcgtg
     7081 cggggcctgt gtcaaggcat gtccgcgcag catcatcgag cttcgcaagc gcaacaagaa
     7141 ggatcgcaag atttatgtct cgtgcgtcaa cagggacaag ggcggtccgg cacgcaaggc
     7201 atgcactgcg gcatgcatag gatgcggcaa gtgcgtgaag gtatgctcct acgaggcaat
     7261 aaccattgag aacaacgtgg cttatattga ctcgttcaag tgcaggcttt gccgcaagtg
     7321 cgtgcaggag tgccccacag gcgctatatt ggagatcggt ttcccggtta aggaagcgaa
     7381 gccggccgag ccggccgatg cagcggttaa ctgattaacg gtgcgctggt caggctgaga
     7441 aaccatcagg ctgaccttaa tactgagaaa gaaaactttc cgtacaatct gaaaaataac
     7501 cataaacgtg ttaaagacat ttccgaaggg tggcatccac ccgccagaga acaagctgac
     7561 ggctgacagg ccggtactta ttcttcccgt tccgtcgcag gtaacggtgc cggtctcaca
     7621 gcacatcgga gctccggcag tgcctgtggt tgagaagggc acagcagtga agaccggaca
     7681 ggtaattgcc caggccaaag ggtttgtctc aacgaacatc cattcgccgg tttccggcaa
     7741 ggtgaacagg atcgacaccg tggttgacac caccggttac aagcagactg ctgttgtgat
     7801 tgatgtcgaa ggggacgaat ggattgattc aattgaccga agtgccacac tggttaagga
     7861 gatcacattg tcgcgtgagc agataataga gaggtgtcag cagtgcggcc tcgtgggcat
     7921 gggaggtgcg accttcccca cccatatcaa gcttaccgtc cctgccggca aaaagtgtga
     7981 catactgatt atcaacggag tggagtgcga gccatatctt acctcagacc acaggctgat
     8041 gcttgagaag ggtgccgagg ttctgactgg cgcctcaata atcatgaagg ccctcggtgt
     8101 tgaaaacgcg gtaataggca ttgaggcaaa caagcctgac gccatctgcc atcttacgga
     8161 acttgcaatg gattacaaag gcattactgt tcagcctctt aaggtcaagt atccgcaggg
     8221 tggtgagaag cagctcatca aggcctgtac cggtcgtgag gttccgtccg gcgggcttcc
     8281 cgtggatgtt ggtgtggtgg ttcagaatgt agggacttcg tttgccgttt acgaggcagt
     8341 tcagaagaac aagccgctga ttgagagggt ggtgaccgtc accgggatgt cggtaaagaa
     8401 ccccggcaat ttccttgtcc gcatcggcac accagtaaca gcgcttattg aggctacagg
     8461 gggcatgcct gatgacaccg gcaaggtggt cagcggtggc cctatgatgg gcaaggcgct
     8521 ggcgatgact gatgttcctg tcacgaaggg aacctcgggg atcatcatgt ttcctgctga
     8581 tgtatcgagg cgtgcagagg tcatgccgtg catcaggtgc ggaaagtgtt catcggtatg
     8641 tgccatgggt ctcgagccgt ggctcatctc ggtcctcgcg cagaaggcga tgtatgagaa
     8701 ggcagagcag gagaagatca ctgattgcat ggagtgcggg gcttgtgcat acacctgtcc
     8761 ggcaaaaaga cccctgcttg actatatcag gcttggcaag gccacggtca taaggatggc
     8821 ccgtgaacgt gttacgaaat agcaaacctc tcacactccc tccctatggt tcaacttgaa
     8881 gagataaaca tattacgaaa gaacaaacat aaatcctcct ctctcctcat tccatggttt
     8941 gacatgaaga gataaactta ttacgaaata gcaaacatta gatatgagca acattttaaa
     9001 cgtatccccg tcaccgcacc agcagagcgc tgaaacaacg cgcaagctga tgatgggcgt
     9061 ggtgatagcc ctggctcctg cccttgcagc ctcaatctgg tacttcgggt tgggggccat
     9121 tatcgttaca ctggtatcag tagtctcatg cgtggcggtt gagtacctga tccagaaatt
     9181 catcctgaag acaaagcctt cggtatcaga cggttcggcg attgtgacgg gacttctcct
     9241 ggcattcaac gtgccttcga acctcccggt tcatataatt atcatcggcg ccatcgtctc
     9301 gataggtata gcaaagatga ccttcggagg actgggtaat aacccgttca acccggcgct
     9361 ggtgggcagg gttttcctgc tcatctcctt cccggtgcag atgaccagct ggcctgtgcc
     9421 atcaggtttc aacaccagtt ataccgacgc agtcacaggc gccactcctc tcgcaatcat
     9481 gagtgaggga atcaggaatg gcgaggccgt ttccaccctg atggagcaa
//
LOCUS       DAOPBK010000098         9467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_6876_length_9467_cov_4.363791, whole genome shotgun sequence.
ACCESSION   DAOPBK010000098 DAOPBK010000000
VERSION     DAOPBK010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6876_length_9467_cov_4.363791"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="lpxA"
                     /locus_tag="PLA17_06960"
     CDS             <1..636
                     /gene="lpxA"
                     /locus_tag="PLA17_06960"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPK85191.1"
                     /translation="GARIGRNCYIFNGASIAAIPQDMKFSGEETTLEIGDNTTVREFA
                     TLNRGTRARGRTIIGNNCLLMAYVHVAHDCQLGDNVIMVASSGIAGEVELGNWVTLGG
                     GTIVHQFVRIGDHAFLGGGSKVRMDVPPYIKADREPLAFMGINSVGLERRGFTKEKMH
                     ELHEIYRAYYNMGMTGTRALEYIDANFPSTPERDYILEFIRHSGRGVVRGR"
     gene            complement(1678..3291)
                     /locus_tag="PLA17_06965"
     CDS             complement(1678..3291)
                     /locus_tag="PLA17_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HPK85192.1"
                     /translation="MKTYIFVALFAIALSSSTLEGQENYIHVPPSHVTVFSSGAQLSG
                     DATVTLQPGTWEYVAGGLSPYIDPNSIQVRGEGDFMIMGVSHRNNYLENPSESDKISA
                     LRERIKALQIRIEDEETATEVLLEKERFLKANYDIVSQKSTITPEQFRAMMDTYGAGM
                     ESVKSAILKKTRILKEYREEKAKLDQQLAGTIDRSKMPAGEIVMTLSGSKPVTGKLKI
                     SYMVMNAGWKPAYDIRVDDITEPAVIIYKANIWQNSGVEWKDVKVSLSNAAPMTAGCL
                     PQLNPWFIDFYQPVMMRGLQGKAAGVNVISKLEREEMASEEVFMADDASAPMPVTVTE
                     SNISFTFDINVPKTIASGGKPETVELQRLTVPATYSYAATPRLASSAYLMGYITEWDK
                     YNLLPGESNIYFSNTFTGKGFINTAELTDTLPVSLGADNSITVKRDRRTDFTSQKLIG
                     SNRVETLSFLISVRNNKNRDVTVKLRDQLPIPRNSNITVEAVELSGGKQNNTTGEVIW
                     DLTVAPRETREIVFTYSVKYPKNKRVILE"
     gene            complement(3419..3925)
                     /locus_tag="PLA17_06970"
     CDS             complement(3419..3925)
                     /locus_tag="PLA17_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK85193.1"
                     /translation="MTSQEFTSGIIDLKGNFHKYALSLTLDRDNALDLVQDTYLKAIQ
                     NQNKFVDDTNLKAWAFTIMKNTFINNYRRKMRENTMMDGTQELYYLNLPSDKGSISPE
                     SAYAGNEIEKAIDALHEDYRLPFRMHLEGFKYEEIAEKLNLKLGTVKSRIFFARKKLM
                     SVLKDYSG"
     gene            complement(4221..>4403)
                     /locus_tag="PLA17_06975"
     CDS             complement(4221..>4403)
                     /locus_tag="PLA17_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85194.1"
                     /translation="TYVITHSGEQEQAIRFIAVITSRGDQEQAIRVIAVITRRGEPGK
                     SIRSIDVLKHRRNYAQ"
     assembly_gap    4404..4503
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4678..5490
                     /locus_tag="PLA17_06980"
     CDS             4678..5490
                     /locus_tag="PLA17_06980"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPK85195.1"
                     /translation="MELKNLPLGETAFYRFEAAGEPRSVILLVHGLGEHAGRYKAWAE
                     RFNERQVTLLAFDLPGHGHTPGRRGVIPSPEKVYDTIDSILGEAAMSFPGVPLFIYGH
                     SLGGGMVLNHLIRRKPSVTGAIVTSPWVILSETPPPLKVFLAKAAGTLMPGLTQSSGL
                     KTEYLSRDPEIVKAYRSDPLVHGQISAGLYLWMSSAAEETLARAHEITVPLLLAHGRN
                     DMITSPSGSVQVADQVPKATLKLWNEGYHELHNDPLKQDHFDFITEWMDTLV"
     gene            5504..6490
                     /locus_tag="PLA17_06985"
     CDS             5504..6490
                     /locus_tag="PLA17_06985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HPK85196.1"
                     /translation="MDLLTGVQMPATASEISYDTPVMFLGSCFANEIGYRMAAGKLPV
                     MTNPHGTLFNPFSVAGAVDRFIAGHTYTERDIYLHQNRYMSLDHHTAFSSYERDVLIE
                     RLNEVSLSASAFTGRASFLFVTFGTSYIYTLRESGAIVANCHKLPSSLFIRRQASWSE
                     IATRWRETLDRLAEANPSLKVWFTVSPVRHLSDGAHANQLSKAHLLLAAEELLSHLFF
                     FKQKTAYEIFMDELRDYRYYAPDMVHPSETGIDYIWEKFSNMFISAHTMRLWNEAARI
                     TRAMAHRLSGGNEGKRETAIFASAMLEKINDLKNRAPFLSLDTEEDYFKKLL"
     gene            complement(6497..7237)
                     /gene="kdsB"
                     /locus_tag="PLA17_06990"
     CDS             complement(6497..7237)
                     /gene="kdsB"
                     /locus_tag="PLA17_06990"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPK85197.1"
                     /translation="MRFTGIIPARYASARFPGKPLALIGGMTMIERVYRQASLVLEET
                     WVATDDERIREAVTAFGGKVVMTSPEHRSGTDRCAEAATVVTDGSENNIIINIQGDEP
                     FIRPEQIRTLMDCFTGNEVMIATLIRRVEPDEDLFNPNQPKVIVRKNMDAICFSRSVI
                     PFFRDSPADEWILKHTYYKHIGLYGYRASTLRELTLLPRSPLEKAESLEQMRWIENGY
                     SIRCAVTPWESIGIDTPEDLDRAARMLK"
     gene            7321..8571
                     /locus_tag="PLA17_06995"
     CDS             7321..8571
                     /locus_tag="PLA17_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK85198.1"
                     /translation="MLMKELQSVKQRFGIIGNHEGLNRALDIAVQVAPTDLSVLITGE
                     SGTGKEVFPQVIHTFSLRKHGPYIAVNCGAIPEGTIDSELFGHEKGAFTGATESRKGY
                     FEVANRGSIFLDEVAELPLSTQVRLLRVLESGEFIRVGSSKVQKTDVRVITATNVDIV
                     QAVSSGRFREDLFYRLNTVPIRVPPLRERGEDIVLLFRKFAVDFAERYRMPAIRLDHQ
                     AREMLLNYYWPGNVRQLKNITEQMSVIEQDRDVTAAKLAHYLPDIQSGRLPVPVRGYA
                     DDKNFSSEREILYKVLFDMKSDMHDLKNLVYDLLTTGDADNLTESGVRAARNLYNQSL
                     LPGQQYEPVQPVSVSYSPPKESRVIEDTEEVIEESLSLIDREKELIKKALEKNNGKRK
                     LAAAELGISERTLYRKIKEYKLDA"
     gene            8564..9079
                     /locus_tag="PLA17_07000"
     CDS             8564..9079
                     /locus_tag="PLA17_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPK85199.1"
                     /translation="MHRIIKIMVPLILLTITAGCGVKITYKFTGASIPVEAKTISVTT
                     FQNRASHVVPGLAQTVTDALIDMCRAQTNLDMVSSGGDLSFEGEITDYKTQPLTVSGN
                     EQAAMNRFSISVRVRYLNGFNDELSFDQTFTRYQEYNSAFDLSDVEAGLTEEIVKMLI
                     EDIFNRAFVNW"
     gene            9088..>9467
                     /locus_tag="PLA17_07005"
     CDS             9088..>9467
                     /locus_tag="PLA17_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85200.1"
                     /translation="MNRNEFNGFISGINLPGPGDLDELRELTSLFPWFHSAHMLFLRG
                     LKENNDIRFDSQLRVSALSVADREVLYHYLFLDPADVADASGEWDVTKPAFAESPVDG
                     VTPVVIPATGVTPVSMEVQITDDVP"
BASE COUNT         2225 a         2264 c         2423 g         2455 t
ORIGIN      
        1 ggtgcgagga taggcagaaa ctgctatatt tttaacggag cctcaatcgc tgcaataccg
       61 caggacatga aattcagcgg agaggagacc actctggaga taggtgacaa cacaaccgtc
      121 agggagtttg ccacactcaa caggggaacg cgcgcaagag ggcgtaccat catcgggaat
      181 aactgcctgc tgatggctta cgttcatgtg gcgcatgact gtcaactggg tgacaatgtg
      241 attatggtag cctcaagcgg gatagccggt gaggtggagc tgggtaactg ggtgacgctg
      301 ggcgggggaa ccatagtgca ccagtttgtg cgtataggag atcatgcttt ccttggtggc
      361 ggatcaaaag tgcggatgga tgtgcctccg tacatcaagg ctgaccgtga gccgctggcc
      421 ttcatgggaa taaacagtgt gggactggaa cggaggggtt tcacaaagga gaagatgcat
      481 gagctgcatg agatatacag ggcttactat aatatgggga tgaccggcac cagagcgctg
      541 gaatatattg acgccaactt cccctctaca cctgagcgtg attatatcct ggagtttata
      601 aggcactccg gaaggggagt agtcaggggg cgttgagccc cttttttgtt tcttacaggc
      661 atgcccggca tcaggtctta ctggtcatac tttgccgggc atccgctctg actgatcttt
      721 ctatgaccag ggatcaggtt gggctgatca tgctttgccc ggggatacta caaccggacg
      781 ggtcatactt tgcgcgtgtg gtcaggtctg actgaccctg ctttgcccgg ggaatccggc
      841 cggactgata ctataatgcc cgggcagttt tatccataac gggaggtata taaaacacga
      901 atgggaacca ttctgtacgt aatagttccc attccggcga gtgacagcca tgactgtcaa
      961 tgccgccatg ctactgttat tgcagccggt tcatctctct tactcctccc ttccctggcc
     1021 tgtcggggtc tgccgtgagg ctgactgctg acctgttccg gtatctcttc tgttatccgt
     1081 agtggccttg cggttttccg ggcacatact gagatgcgga gttatgaacc cttgaagcat
     1141 gacacccccc gaaggggcag ctgctgtcat gcccgggtca tctccatcct atcctcgccg
     1201 gttcggttgg catgatgacc ctctctctga acgggcacat ttgctgcctt attagctttc
     1261 agccttgcat ctgtgttgct atcctctcgc ggctgttaca ccgtctgatg atcgctccgt
     1321 cagaccgcca tgatgggaag gcttctcagc ctgaaccctt gacggacgct tccgaaccgt
     1381 gattcttcag cgtgagactc tgaagtctga tgttaattca gagtgataat taaaccaatc
     1441 caaagaactt tcatgcttca gagcgactct ttatcgctca catggtagga caaatataac
     1501 cagataacat gtgcaaatca aatttttcat gtaaataata cgattaagaa aacaacacga
     1561 aatccacaac tttatcaaca acaacaagta gcttcaggtc aatggtttat cataaaatga
     1621 acaaggtttt caacggctgt tgaaaacctt gcctgtcagt cgtttgccaa ctctctgtta
     1681 ttcaagtata actctcttgt ttttcgggta cttcacgctg taggtaaaga caatttccct
     1741 ggtttcacgg ggtgcaacag tgaggtccca gataacctcg cccgtggtgt tgttctgctt
     1801 gccgccgctt agctcaacgg cctcaacggt gatattgctg ttccggggta ttggtaactg
     1861 gtcacgtaac ttcactgtca catccctgtt tttgttgttc ctgacagaga tcaggaacga
     1921 gagggtctca actctgttgg acccgatcag tttctgcgag gtgaaatctg tgcgccggtc
     1981 cctcttcacg gttatgctgt tgtctgcacc cagtgatacc ggaagggtgt cagtcagctc
     2041 ggcggtgttg atgaatccct tgccggtgaa ggtgttgctg aagtagatgt ttgactcacc
     2101 cggcaggaga ttgtatttat cccattccgt gatgtacccc atcaggtatg ccgaagaggc
     2161 aagcctgggc gttgcggcat agctgtaggt ggcaggcacg gtaagcctct gcaactccac
     2221 tgtctccggc ttgccgcctg atgcaatggt tttgggcaca ttgatatcga aagtgaagct
     2281 gatgtttgac tctgtcaccg tcacaggcat gggggcactg gcatcatctg ccataaatac
     2341 ttcttccgaa gccatctcct cacgctccag ttttgaaatg acattaactc ctgctgcttt
     2401 accctgcaat cctctcatca taaccggctg gtagaagtca atgaaccacg gattcagctg
     2461 aggcaggcag ccggcagtca tcggtgcggc atttgagagg cttactttca catccttcca
     2521 ctccacgccg ctgttctgcc agatattcgc cttgtatatg attacggccg gttcggtaat
     2581 atcatcaacc ctgatgtcat atgccggctt ccatcctgca ttcatcacca tgtatgatat
     2641 cttcagcttc ccggtaacgg gcttgctgcc ggaaagggtc atgacaatct cgccggcggg
     2701 catctttgag cggtcaatgg tgccggcaag ctgctggtca agctttgcct tctcttccct
     2761 gtactccttc agaatcctgg tttttttgag aatggcactc tttacactct ccatgccggc
     2821 gccataggta tccatcatgg ccctgaactg ttcgggagtt atggtcgact tctgactgac
     2881 aatgtcatag ttggccttga gaaatcgctc cttctccagc agcacctctg tggccgtttc
     2941 ctcatcctca atcctgatct gcagggcttt tatcctttcg cgcagagcgc tgatcttatc
     3001 tgactctgaa ggattctcaa ggtaattgtt ccggtgtgac acacccatta tcataaaatc
     3061 accctcacct ctcacctgga tgctgtttgg atctatatat ggcgatagtc ccccggcaac
     3121 gtactcccat gtcccgggct gaagagttac ggttgcatca ccggagagct gggcaccgct
     3181 actgaaaacc gtaacatgac ttggaggaac atgaatataa ttctcctgcc cctcaagagt
     3241 ggaagaagaa agcgcaattg caaacaacgc aacaaagatg tatgttttca tgctgtcgga
     3301 ttttttataa aggtattgaa aaagagattt gtgatcaagg acgacaaaaa tcctgccgaa
     3361 aaagaaactg ccggacaaat gtaatccggc agttcatctt caccatcctt tgtctaacct
     3421 aaccactgta gtctttaaga acactcatga gtttcttgcg tgcaaagaaa atgcggcttt
     3481 tgaccgtgcc gagcttcagg ttgagcttct cagctatctc ctcgtacttg aaaccttcaa
     3541 gatgcatgcg aaaggggaga cggtaatcct catgaagcgc gtcaatggcc ttctcaatct
     3601 cattgcctgc ataagccgac tccggtgaaa tgcttccctt gtcagagggg agattgagat
     3661 agtaaagctc ctgcgtgccg tccatcatag tgttttccct catcttgcga cggtaattgt
     3721 tgatgaaggt gtttttcatt atcgtgaagg cccaggcctt gaggttcgtg tcatcaacga
     3781 atttattctg gttctgtatt gccttcaggt atgtgtcctg aaccaggtca agtgcattgt
     3841 cgcggtcaag ggtgagactg agggcatact tgtgaaaatt gcctttcaga tctattatgc
     3901 ctgatgtaaa ttcctgtgaa gtcatctctg tggtttttat ttgttggtgc aaagttaaac
     3961 agttatttcg gataacaata ccttaatatc ttattatatt gataaaacag gttctcttta
     4021 cctgttttct aaactttaat gcaaacaaaa tcaggcatat aaatgtgata agtctaaatt
     4081 aagataacga agtatttttt tatttgcggt ctgcccggaa caggaaggaa agatggagga
     4141 gcaagtggcc aggcttgtat aaatagcata atgacagcga gatacgtatt aaaattactc
     4201 tgatggcttt acctggctct ttactgtgcg taattacgtc tgtgcttgag cacgtctatg
     4261 gatctgatgg atttgcctgg ctctcctctg cgcgtgatca cggcaatgac tctgatggct
     4321 tgctcctgat ctcctctgct cgtgatcacg gcaatgaatc tgatggcttg ctcctgctct
     4381 ccactatgcg tgatcacata tgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnncggcaat gaatctgatg gcttgctcct gctctccact atgcgtgatc acatatgtgc
     4561 gcgatcacgt ctgtgaatct gatggcactg ttttgctctc ctccgtgtgt gataacggat
     4621 agaggcgaac acgctccact cttctccggt tttaattatc tttacaaaaa agtggttatg
     4681 gaattgaaaa atctacctct gggagagaca gctttttacc ggtttgaggc tgcaggagag
     4741 ccccggtcgg tcattctgct tgtacatggt ctgggtgagc atgccggcag gtataaggcg
     4801 tgggctgaac ggttcaatga gaggcaggtg actctgctgg cgtttgatct gccgggccac
     4861 gggcatacac caggccggag gggtgtgata ccatcgccgg agaaggttta tgataccatt
     4921 gacagcatac tgggcgaggc tgccatgagc ttcccgggcg tgccgctctt catttatggt
     4981 catagtctcg gagggggcat ggtgctcaat cacctcatcc gcagaaagcc gtcagtgacc
     5041 ggtgcaattg ttacctcacc ctgggttatc ctttctgaaa caccacctcc gctgaaggtt
     5101 tttcttgcga aggcagccgg tacgctgatg ccgggactga cccagtcatc aggactgaag
     5161 actgaatacc tctcgcgtga cccggagatt gtaaaagctt accggagtga tccgctggtg
     5221 catggtcaga tctcagccgg gctttatctc tggatgtcat ctgcagctga agagactctt
     5281 gcccgcgcgc atgagataac cgttcccctg ctgctggcac acggacgcaa tgacatgatc
     5341 acctccccgt cagggtcggt acaggtggca gaccaggttc caaaggctac cctgaagctg
     5401 tggaacgaag gctaccatga actgcacaat gatccgctga agcaggatca cttcgacttc
     5461 atcacggagt ggatggacac attggtataa ttatttttgg gtgatggacc ttcttacagg
     5521 tgtgcagatg cctgccacag cttcggagat cagttatgac actccggtta tgtttcttgg
     5581 ctcatgcttt gccaacgaga ttggctaccg tatggcagcc ggcaagcttc cggtcatgac
     5641 caatcctcat ggcactcttt tcaatccgtt ttcagtggcc ggggctgttg acaggttcat
     5701 cgccggccat acctataccg aaagggatat ttaccttcac cagaacaggt acatgagcct
     5761 tgatcatcac accgccttct catcttatga gcgtgatgta cttatagaga ggctgaatga
     5821 ggtgagtctc tctgcttctg ccttcacagg gagggcttcg ttcctttttg tcacgttcgg
     5881 cacatcctat atatatacac tcagggaaag cggagccatt gtggccaact gccacaagct
     5941 cccttcctca ctgtttatcc gcaggcaggc atcatggtct gagattgcca cccgctggag
     6001 ggagacactt gaccgcctgg ctgaggccaa tccctcactg aaggtatggt tcaccgtcag
     6061 tcctgtccgc cacctgagtg atggcgctca tgcaaaccag ctgagcaagg ctcatcttct
     6121 gctggcagca gaggaactgc tctcgcatct tttttttttc aagcagaaga cggcatacga
     6181 gatattcatg gatgaactcc gtgactaccg ttattatgcc ccggatatgg ttcatccctc
     6241 tgagacgggg attgactata tatgggaaaa gttcagcaat atgtttattt cagcccacac
     6301 catgagacta tggaacgagg cagccaggat cacccgtgcc atggcgcacc ggttatccgg
     6361 aggaaatgaa ggaaagcgtg aaactgccat tttcgccagc gccatgcttg agaaaatcaa
     6421 tgatcttaaa aaccgggccc cgtttctcag ccttgacact gaggaagatt atttcaaaaa
     6481 gcttttgtag cctgaattat ttaagcatcc ttgctgccct gtcaagatct tcgggggtgt
     6541 caatgccgat actctcccag ggggtgacgg cgcagcggat ggaatagccg ttttctatcc
     6601 atctcatctg ttcaaggctc tcggccttct cgaggggcga ccgggggagg agggtcagct
     6661 ccctgagggt tgaggcgcga tagccataga ggccaatgtg cttgtaatag gtgtgtttca
     6721 gaatccattc atctgcaggt gaatcacgga agaaaggaat aactgaccgg ctgaagcaaa
     6781 tggcatccat gttttttctg acaatgacct tgggctggtt gggattgaaa agatcttcat
     6841 ccggctcaac cctgcggata agggtcgcta tcatcacctc attgccggtg aagcaatcca
     6901 tcagagtcct gatctgttcc ggcctgataa acggttcatc gccctggata ttgataatga
     6961 tattgttttc gcttccgtcg gtgaccacag tagctgcttc ggcacaacgg tcagttccgc
     7021 tccggtgctc tggtgaggtc attacaacct tcccgccgaa ggcggttacc gcttcgcgga
     7081 tgcgctcatc gtcagttgcc acccaggtct cctccagaac cagtgaagcc tgccggtaca
     7141 ctctctcgat cattgtcatc ccgccgatga gggcaagagg ttttccgggg aagcgtgctg
     7201 aggcatatcg tgcagggata atccccgtaa accgcagtga atggcactct ttcatggctc
     7261 caaattacta aaaacagctt taacataatg taaaaaagtc gtaaattcgc agttcattgg
     7321 attttgatga aggaattaca gagcgtaaaa cagaggtttg gaataattgg caatcatgag
     7381 ggactgaaca gggctcttga catagctgtt caggtggccc ccacagacct ctctgtactt
     7441 ataacaggtg aaagcggaac cgggaaggag gtttttccgc aggtgataca caccttcagc
     7501 ctgcgcaagc acggtccgta tatagcggtg aactgtggcg ccataccgga gggaacgatt
     7561 gattcagaac tcttcggaca tgagaagggt gcttttaccg gggcaacgga gagccgcaag
     7621 ggctacttcg aggttgcaaa cagaggttcg atattccttg atgaggtggc tgaactgccc
     7681 ctctctacgc aggtcaggct gctcagggta ctggagagcg gtgaattcat aagggttggt
     7741 tcatcaaagg ttcagaagac cgatgtcagg gtcattaccg ccaccaatgt tgatatagtc
     7801 caggcagtaa gttcgggaag gtttcgtgaa gaccttttct acaggctgaa tacggtaccc
     7861 atacgggtgc cgccccttcg tgaaagaggt gaagatatcg ttttgctgtt ccggaagttc
     7921 gctgttgatt tcgcggaacg ctaccggatg ccagccataa ggcttgatca ccaggccagg
     7981 gagatgttgt tgaactacta ctggccgggc aatgtaagac agctgaagaa tatcactgaa
     8041 cagatgtcag taattgagca ggaccgtgat gtgactgccg ccaaacttgc acattatctt
     8101 ccggatattc agagcgggag gcttcccgtt cctgtaaggg ggtatgccga tgataagaat
     8161 ttcagcagcg aaagggagat tctttataag gtgttgttcg acatgaagag tgacatgcat
     8221 gacctgaaga atcttgtcta tgacctgctg accaccggtg atgctgacaa cctgacagaa
     8281 agcggtgtgc gtgctgcgcg aaacctttat aaccagagtt tgcttcccgg ccagcagtat
     8341 gagccggtac aaccggtgag tgtaagctac agcccgccga aggagagcag ggtgattgag
     8401 gacaccgagg aggtgataga ggagtctctc tcactgattg accgtgaaaa ggaactcatt
     8461 aagaaggcac ttgaaaagaa caacggcaag agaaagctgg cggcggctga actggggata
     8521 tccgaaagaa ctctctacag aaaaataaag gagtacaagc ttgatgcata ggataataaa
     8581 gataatggtg ccgctgattc tgctgacgat tactgccggc tgcggagtta aaatcacata
     8641 taaattcacc ggtgcctcca taccggtgga ggcaaaaacc atcagtgtga caacctttca
     8701 gaacagggca agccatgtgg tacccggact ggcacagacc gttaccgatg ctcttatcga
     8761 tatgtgcagg gcgcagacca atcttgatat ggtatcatcg gggggcgatc tgagtttcga
     8821 gggagagatc actgactaca agacgcagcc gcttactgtc agcggcaatg aacaggctgc
     8881 catgaacagg ttctcaatct ccgtgcgggt caggtatctg aatggattca atgatgaact
     8941 cagctttgat cagaccttca cccgttatca ggaatacaac agcgcgtttg atctgagtga
     9001 tgttgaagcc ggcctgacag aagaaattgt gaagatgctt atcgaagata tatttaacag
     9061 ggcgtttgtc aactggtaat tctgatcatg aacaggaatg aattcaacgg gttcatttca
     9121 ggcattaatc tcccgggtcc gggagacctg gatgagttga gggagcttac ttcccttttc
     9181 ccctggtttc attctgccca tatgcttttt ctccgggggc tgaaggagaa caatgacatc
     9241 cggtttgact cacagctccg ggtatcagct ctttcagtgg ctgacaggga ggtgctttac
     9301 cactatttgt tccttgatcc tgctgatgtt gctgatgctt caggtgaatg ggatgttacc
     9361 aaacctgcat ttgctgagtc gccggttgac ggggttaccc cggtggttat cccggcaaca
     9421 ggtgtaactc cggtatctat ggaggtccag atcactgatg atgtgcc
//
LOCUS       DAOPBK010000099         9322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7001_length_9322_cov_5.850869, whole genome shotgun sequence.
ACCESSION   DAOPBK010000099 DAOPBK010000000
VERSION     DAOPBK010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7001_length_9322_cov_5.850869"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLA17_07010"
     CDS             complement(<1..810)
                     /locus_tag="PLA17_07010"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HPK85201.1"
                     /translation="MRNSFWNLQNKRPGNTRKGAPSIILILSALLTAVLLSCSQDPVK
                     IGIGLLPDSDFVEIGSTDTIGIRAYTMYDELSVSNDSTVMIIGSINDDYFGDTYCDFV
                     TQLRLMSKWPAKPFVIDSVMLIMLPSAVSGDDSTSTHRIRLYETGTRLSDGDEFYSGQ
                     DPDTIRFLGEYDLPRMKVGELLRVKLDNSVGEHLLRDTTKLSAGVPFYEEYFKGLYCM
                     MKSSPNRVMLEMNASTDVRTDPLGITIYYKSDTLRYTFSFVATPRAVNYNRF"
     gene            complement(779..1600)
                     /locus_tag="PLA17_07015"
     CDS             complement(779..1600)
                     /locus_tag="PLA17_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPK85202.1"
                     /translation="MESRRVLFVSQEIMPFLGETKLSRISRDLPQGIQERGKEIRTFM
                     PRYGSINERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQSARMQVYFIDNDDYFHR
                     KHTVADAAGNEYEDNDERAIFFARGVIETVRKLRWSPDIVHVHGWLASLVPLYIRKLY
                     SDDPIFSKAKSVYSVYNNGFKKPLNASLTDKLQADGIDTGSLKSIEGGADFLNLSTLA
                     AENSDGIIFGCEKIDEQVKKRITAMGKPLLEYQGEENYIDVYNDFYEKLLLESAK"
     gene            1778..2635
                     /gene="panC"
                     /locus_tag="PLA17_07020"
     CDS             1778..2635
                     /gene="panC"
                     /locus_tag="PLA17_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPK85203.1"
                     /translation="MIVLSTVKEVREHYRISVLRNPGFVPTMGALHKGHLSLVARATE
                     RCRMVAVSIFVNPTQFNDPNDLLKYPRTLDDDLEALSGMLRENDFVFVPSVEEIYPEP
                     DSRQFEFGDLDKVMEGVHRPGHFNGVAQVVSRLFDIIRPSCAFFGQKDFQQLTIIREL
                     ARREYPKIEIIGCPIVREHDGLAMSSRNRRLLTQHREKAGEIYRTLIAAAAMISDYEI
                     NEIREFVTDRINMIPDFRLEYYEIVEEETLKPVHSVFEMSPEKKYYGCIALWAGEIRL
                     IDNIEIRLR"
     gene            2686..3036
                     /locus_tag="PLA17_07025"
     CDS             2686..3036
                     /locus_tag="PLA17_07025"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPK85204.1"
                     /translation="MTIEILKSKIHNVTVTGANLNYMGSVTIDEELMDAAGLVEHEKV
                     HVLNRNNGERLVTYVIRGKKGSGEICLNGPAARKIQVGDVVIIMAFATITPEEARSFV
                     PRIIFPDTTTNKII"
     gene            3040..4077
                     /locus_tag="PLA17_07030"
     CDS             3040..4077
                     /locus_tag="PLA17_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPK85205.1"
                     /translation="MNNSLKHFLRAVLFLSLAALLLWLSFRDIRFDDLWSVLRKANYW
                     WLIPALIFSYLSFIIRARRWNLLIEPLGFKPTLAHSSHSVITGYFANMLFPRLGEVAK
                     CATLSRKENIPFDKLVGTMLVERTIDILTVLIILGAIVAIGSTATGSFLSENVFMPAE
                     RKISSSVGSTTIITLIVLLLAVTAVVMFFTLRERLSGRPLFKKMYSFSDGLFSGLKSI
                     LRLKRGWEFLIMTILLWVCYFLMSYFPLLCLESTAHLGAGATMFILVIGSFGMAAPVQ
                     SGLGAYHWIISRGLLVAYAIPLEEGLAYATLEHESQMILIAIAGAISLYALFGSKGGK
                     VLSSVVTEKKV"
     gene            4123..5475
                     /gene="radA"
                     /locus_tag="PLA17_07035"
     CDS             4123..5475
                     /gene="radA"
                     /locus_tag="PLA17_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPK85206.1"
                     /translation="MAKNKSVYVCQNCGAESPKWIGRCPSCHEWNSYREEIISTARTP
                     APGRDAVRRKPELLSGIEANENDRITTGIGEVDRILGGGMVKGSLILMGGEPGIGKST
                     LALQIALARPERTVLYVSGEESEGQVKLRAKRLEGKNERCFIYNETELESVLTQTGVI
                     KPELLIIDSIQTLSSSMLESSAGSVTQVRECAAMMLRYAKESGVPVILIGHITKDGTL
                     AGPKVLEHIVDVVLQFEGDGNYLFRILRPVKNRFGSTAEIGIFEMRSKGLLEVTDPSE
                     LFIRRDRDPASGVTIAATVDGIRPFMIETQSLVSSAVYGTPQRSSTGFDTRRLNMLLA
                     VLEKRAGFRLGVKDVFLNIAGGLKVSDPAVDLAIAAAVLSSNLDVPVDHHSAFAAEVG
                     LSGEVRPVARIEQRIMEAARMGFTGMVISGFHRDVKDVPGLKISKVYKIEGLVKLLFS
                     "
     gene            complement(5633..7972)
                     /locus_tag="PLA17_07040"
     CDS             complement(5633..7972)
                     /locus_tag="PLA17_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPK85207.1"
                     /translation="MRDKKFRKNIIWFWVIISLPAVFLLTLFLLISANKLGPIPSFEE
                     LENPDNSLAAEVYSEDNVLLGKFYIQNRTWTDYEDISPYVIDALISTEDIRFKRHSGI
                     DFRGLARVLVKTIMLGKNTGGGSTITQQLAKNLFLPRDLSNDPAIVRASRLAIAKFKE
                     WQTAVKLERSYTKEEIITMYLNVFDFIYNAVGINSAARIYFNTTPDSLNIEQSAMLVG
                     MLKNSVAYNPLRNEESALKRRNVVISQMERYGYISRAVADSVSQLPLGLDLREDSHNT
                     GLATYFREYIRSTMSSREPQRERFYSDDTYNEALWRWQNDPLYGWCYKNTKPDGSPYN
                     IYRDGLRIYTTLNSKMQTYAEEALTEHLSQNLQPVFDKRAKNYRNPPYSNDLTTAQVN
                     QIMARSVRQSDRYQSMRRAGVPEDSITLAFNTPVPMKLFSWEGERDTVMTPLDSIRYY
                     KFFVRSAFMAMDPHTGHVKAYIGGPDFRYFKYDAVTQQKKQVGSTIKPFLYTLAMQDG
                     YSPCYEVENIERTFEVNDTIWRPRSSGPVEYHGKMVTLKWGLAQSENYISAWLMKQFS
                     PQAVVDIMQRMGIRSFIDPVVSIFLGTSDLSVEEMVGAYGTFVNKGVYTRPLYVTRIE
                     DRNGNVISTFTPFIDEVISEDDAYLMTSLLQGVVTNGTAVRLRLTYKLTNQMGGKTGT
                     TQNHSNGWFMGVMPDLVAGVWTGWEDQAIHFEDLSMGQGANMALPVFGIFMQKLLADK
                     DFSVIAESQFEAPPGFNVELDCDKVVREQQGNPFRQREY"
     gene            8085..>9322
                     /gene="topA"
                     /locus_tag="PLA17_07045"
     CDS             8085..>9322
                     /gene="topA"
                     /locus_tag="PLA17_07045"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPK85208.1"
                     /translation="MADNLVIVESPAKAKTIEKFLGKDYTVVSSFGHIRDLVRNKLGV
                     EVDNNFAPVYEIPEDKKKVVAELRKLADKATTVWIASDEDREGEAIAWHLISVLGLDP
                     ATTRRIVFHEITKDAITRAVESPRQVDMNLVNAQQARRILDRLVGFELSPVLWKKVQP
                     SLSAGRVQSVAVRLLVDREREIIGFVADSAFRVTGTFVGTNSEGSDVQLKAECQKKFS
                     TEQEAGLFLEKCDASAFTVENIVVKPGTRSPAPPFTTSTLQQEAYRKLGFSVAQTMSV
                     AQKLYEAGKITYMRTDSTNLSSLALNTAREVITNEYGAGYSRVRQFKTHTRGAQEAHE
                     AIRPTYFDRKEVTGNNNEKKLYELIWRRAIASQMADARVEKTTITIGMSGSPVSFTAT
                     GEVILFDGFLKVYTESFDGNV"
BASE COUNT         2324 a         2371 c         2351 g         2276 t
ORIGIN      
        1 gaacctgtta tagttcactg ccctgggcgt agccacaaat gagaaagtgt atctgagtgt
       61 gtcagattta taatagatag tgatacccag cgggtcagtg cggacatccg tggatgcatt
      121 catttcaagc ataaccctat tgggtgagct tttcatcatg caatagagtc ccttaaaata
      181 ctcctcgtag aatggtactc cggcggatag tttggtggtg tctctcagca ggtgctcacc
      241 aaccgagttg tcaagtttca cccttaacaa ttctccgact ttcattctgg gaagatcgta
      301 ttcgccaagg aatctgattg tgtcagggtc ctgacctgaa taaaactcat cgccgtcact
      361 aagcctggtt cctgtttcat agaggcgtat ccggtgggtt gatgtggaat catcacccga
      421 aacagctgaa ggcaacatga tcagcataac ggagtcgatg acaaaaggtt tggccggcca
      481 cttgctcatg agcctgagct gtgtcacgaa gtcacagtat gtgtcaccga aatagtcatc
      541 attgattgat ccgatgatca tcaccgtgct gtcgttcgag acggagagtt cgtcatacat
      601 tgtatatgcc ctgatcccga tggtatcggt tgagcctatt tccacgaagt cgctgtcagg
      661 cagcagaccg atgcctattt tgacggggtc ctgtgaacat gacaacaata ctgctgtgag
      721 cagggcggaa aggatcagta tgattgaagg cgctcccttg cgggtattgc cgggtctttt
      781 attttgcaga ttccagaagg agtttctcat aaaagtcatt gtatacatct atataattct
      841 cctctccctg gtactcaagc agaggctttc ccatggcagt gatccttttc tttacctgtt
      901 cgtcaatctt ctcgcacccg aatataattc cgtcagagtt ctcggcggca agggttgaga
      961 gattgagaaa atcagcgcct ccttctattg acttgagaga gcctgtgtca atcccgtctg
     1021 cctggagctt gtcagtcagt gaagcattta acggcttttt aaacccgtta ttataaaccg
     1081 aatagactga tttggccttg ctgaatatcg ggtcatcgga atagagcttc ctgatataga
     1141 ggggcaccag cgatgcgagc cagccgtgaa cgtgtactat gtcagggctc cagcgcagtt
     1201 ttcttaccgt ttcaataact ccacgggcaa agaaaatggc tctttcgtca ttgtcttcgt
     1261 actcattgcc tgcagcatca gctacagtat gcttgcgatg gaaataatca tcattgtcaa
     1321 taaagtacac ctgcatccgg gcactctgaa tggaggctac cttgataata agaggatggt
     1381 cggtatcatc aatgatcagg ttcatcccgg agaggcggat gacctcatgc agctggtttc
     1441 ttctctcgtt gattgagcca taccggggca tgaatgtacg aatctctttc ccccgttcct
     1501 gtatgccctg tggcagatca cggctgatcc ttgaaagctt cgtttcaccg agaaagggca
     1561 taatctcctg tgaaacaaac aatacccttc tgctctccat taatacctcc cgatatacct
     1621 taggatttaa tcaaagtgca aagataataa aaaaaactaa attgatttcc aagcttagag
     1681 acaacgggtt actgacaaaa atggtgttat tttgcagtac cggacacaat gcctttgccg
     1741 gtatacagtc cggccttcat acaaaaaaac aataggaatg atagtactgt caactgtcaa
     1801 ggaagtaagg gaacactaca ggatcagcgt cctgcgcaac cccggtttcg tgcctaccat
     1861 gggcgcactc cataagggcc atctgtcact ggtggcaaga gcaacggaga gatgccgaat
     1921 ggtggccgtg agtatcttcg tcaatcccac acagttcaat gacccgaatg acctgctcaa
     1981 atatcccagg accctcgacg acgacctgga ggcgctctca ggcatgctca gggaaaatga
     2041 ctttgtcttc gtgccgtcag tggaggagat ttacccggaa cctgacagcc gccagttcga
     2101 gttcggtgac ctggataaag taatggaagg cgttcacagg ccaggccatt tcaacggcgt
     2161 ggcacaggtg gtatcacggc tctttgacat catcaggcca tcatgcgcct ttttcgggca
     2221 gaaggacttc cagcagctga caatcatcag ggagctcgca aggcgcgaat acccgaagat
     2281 tgaaatcatt ggctgcccca ttgtcaggga acatgacggg ctcgccatga gcagccgcaa
     2341 ccgcaggctc ctgacacagc accgcgaaaa agcaggcgaa atataccgta ccctgatagc
     2401 tgcagcagcc atgatctctg actatgagat aaatgagatc agggagttcg tgactgaccg
     2461 aatcaacatg atccctgact tcaggcttga atactatgag atagtggaag aagagacgct
     2521 gaaaccggtt cattcggttt ttgagatgtc accggaaaag aagtattacg ggtgcatagc
     2581 cctgtgggcc ggagaaataa gactgattga caacattgag atacgattgc ggtaaatcaa
     2641 aggataacta tatttgcacc gtcaaaaagc aatctgattc agaacatgac aatagaaata
     2701 ctcaaatcaa aaatacacaa tgtcaccgtc accggcgcta acctcaatta catgggaagc
     2761 gtcaccatcg atgaagagct gatggatgca gccggccttg ttgaacatga gaaggtccac
     2821 gtgctgaaca ggaacaacgg tgaaaggctt gtaacatatg ttatcagggg aaagaaaggg
     2881 tcaggggaga tatgcctcaa cggccctgct gcaagaaaga tccaggttgg cgatgtggtt
     2941 atcattatgg cttttgcaac tatcactccg gaggaagccc gttcatttgt gccccggatt
     3001 atctttcccg atacaactac caacaaaatt atctgactct tgaataacag tttgaaacac
     3061 ttcctgaggg cagtattgtt cctgtctctg gctgcgctgc tgctatggct gtcattcagg
     3121 gatatcaggt ttgatgatct gtggtctgtg ctccgcaagg ccaactactg gtggctgata
     3181 cctgccctga tcttctcgta tctcagcttt attatcaggg cacggcgctg gaatctgctc
     3241 atagagccgc tgggattcaa acccaccctt gcccattcaa gtcattccgt cattaccggt
     3301 tattttgcca acatgttatt tcccaggctt ggcgaagtgg caaaatgcgc taccctctcc
     3361 cgcaaggaga atatcccgtt tgacaagctt gtggggacaa tgctggttga acgtacaatt
     3421 gacatactta cggttctgat tatacttgga gccatcgtcg ccattggaag taccgctacg
     3481 ggaagcttcc tctccgagaa tgtctttatg ccggctgaaa ggaagatatc atcatctgtc
     3541 ggatcaacca ccataattac actgatagtg ctgttactgg cagtgacagc cgttgttatg
     3601 ttcttcaccc tccgggaacg gctgtcgggc aggccattgt tcaaaaagat gtactccttc
     3661 tctgacgggc tcttttcagg gctcaaatca atcctcaggc ttaaacgcgg atgggagttc
     3721 ctgatcatga ccattctgct ctgggtctgt tactttctga tgtcatactt ccccctgctc
     3781 tgtcttgaat caacagccca cctcggcgca ggagccacaa tgtttatcct ggtcataggc
     3841 agtttcggca tggccgcacc tgttcagagc ggactggggg cttatcactg gattatttcc
     3901 cgcgggctcc tcgtggcata cgccattccc cttgaggaag ggctcgccta tgcaacgctc
     3961 gagcatgagt cacagatgat tctcatcgct attgccggcg ccatttcgct ttatgcgctc
     4021 ttcggcagca agggaggaaa ggtactctcc tcggtagtca ctgaaaaaaa ggtctgatac
     4081 cttttctttt agtaaatttg gctgttgcat attccttttc ccatggcgaa gaacaaatca
     4141 gtctacgtgt gccagaactg cggcgcggag tcaccgaaat ggattggacg ctgtccctca
     4201 tgccacgagt ggaacagcta ccgcgaagag ataatcagca ctgcacgaac cccggcgccc
     4261 ggaagggatg ccgtgcggcg aaaaccggaa ctcctgtcgg gaatagaggc gaacgagaat
     4321 gaccggataa ctacaggcat cggagaggta gaccggatcc tcgggggagg aatggtgaaa
     4381 ggctcactga tactcatggg cggtgagccc ggcatcggca aatcaaccct ggcactgcag
     4441 atagcgctgg ccaggccgga aagaacggta ctctacgtct ccggagagga gagcgaggga
     4501 caggttaagc tcagggcaaa acggctcgaa gggaaaaatg agaggtgctt catctacaac
     4561 gagacagaac tcgagagtgt tctgacacag acaggagtaa tcaaacccga actgctgatc
     4621 attgactcaa tccagacact cagctcctct atgctcgagt catcagccgg ctctgtcacc
     4681 caggtcaggg agtgtgccgc catgatgctt cgctatgcca aagagtcagg tgtgccagtg
     4741 atactgatag gccatataac aaaggatggc accctggccg gcccgaaggt tctggagcat
     4801 attgtagatg tggttctgca gttcgaggga gacggcaact acctcttccg tatcctcaga
     4861 cctgtgaaaa accgtttcgg atcaacggca gagatcggca tcttcgagat gagatcaaag
     4921 ggcctgctcg aggttaccga cccctcggag ctgtttatac gccgtgaccg tgacccggcc
     4981 agcggggtga ccattgccgc caccgtagac gggataagac ccttcatgat tgagacccag
     5041 tcactggtaa gcagcgccgt ctatggcacc ccgcagcgaa gctccaccgg ctttgacacc
     5101 aggagactga acatgctgct tgccgtactt gagaagagag ccggattcag gctgggggtg
     5161 aaggatgtct tcctcaatat tgcaggcgga ctgaaggtaa gtgatccggc tgtagacctg
     5221 gccattgccg cagccgtgct ctcatcaaac ctcgatgtgc cggttgacca tcattccgcc
     5281 tttgccgccg aagtgggatt atcaggagag gtaagacctg ttgcgaggat agaacagcgc
     5341 attatggaag ctgccaggat gggattcacc gggatggtca tctcgggatt tcaccgtgat
     5401 gtaaaggatg tacccggact gaaaatatca aaagtgtaca agattgaggg gctggtgaag
     5461 ttgctcttct catgaatcat caggtataca gactcactcc tctgccggag tctcaaactg
     5521 ccgagtggta ctgtcatcct gactgatgac tactggctgg aactgccgcc ctgaccgttg
     5581 gctggcaatt ccgacctgac cgctgactga caccgccacc ctgacttcag tactagtatt
     5641 ccctctgcct gaaaggattg ccctgctgct ccctgaccac cttgtcacag tcaagctcta
     5701 cgttgaatcc cgggggcgct tcaaactgtg attcagctat cactgagaaa tccttgtcgg
     5761 ccagcagctt ctgcataaag atgccgaata ccggcagagc catgttggct ccctgcccca
     5821 tcgagagatc ttcgaaatgg attgcctgat cctcccatcc cgtccacaca ccggcaacca
     5881 gatcaggcat gacacccata aaccatccgt tggagtggtt ctgtgtggtt ccggtcttcc
     5941 ctcccatctg gttggtaagc ttgtaggtaa gcctcagtct cactgcggta ccgttggtta
     6001 ccacaccctg cagcagcgag gtcatgagat aggcgtcatc ctcgctgata acctcgtcaa
     6061 tgaacggtgt gaaggtagat atgacattcc cgttgcggtc ttctatccgc gtcacataga
     6121 gcggccgggt atacaccccc ttgttgacaa aggttccata ggccccgacc atctcctcta
     6181 cggagagatc ggaggtaccg aggaatatag agaccacggg atcaataaag ctccttattc
     6241 ccatacgctg cattatgtca accaccgcct gcggcgagaa ctgcttcatc agccaggctg
     6301 atatgtagtt ctctgactgt gccagtcccc acttcagtgt caccatcttg ccatgatact
     6361 caacaggacc tgagcttctg ggccgccata tcgtgtcatt gacctcgaag gtacgctcaa
     6421 tgttctcaac ctcgtagcat ggcgaataac catcctgcat ggccagtgtg tagaggaagg
     6481 gcttgatggt ggaacctacc tgtttcttct gctgggtgac agcatcatac ttgaagtatc
     6541 tgaaatcagg ccccccgata tacgccttca catgccccgt gtgggggtcc atggccatga
     6601 atgctgatct cacaaaaaac ttgtaatacc ttatcgagtc aagcggcgtc ataactgtgt
     6661 ctctctcccc ctcccatgag aagagtttca tcggcacggg tgtgttgaac gccagcgtta
     6721 tcgaatcctc aggtacaccg gccctcctca tgctctgata ccggtcgctc tgcctgactg
     6781 accttgccat gatctggttc acctgagccg tggtgaggtc atttgagtag ggcggattcc
     6841 ggtaattctt tgccctcttg tcaaataccg gctgaaggtt ttgggaaagg tgttctgtaa
     6901 gagcctcttc cgcataggtc tgcatcttcg agttaagggt ggtgtagatt ctcagtccgt
     6961 cacggtagat attatatggt gagccgtcag gcttggtatt cttgtagcac cagccataga
     7021 gcggatcatt ctgccagcgc cagagagcct cattgtaggt atcatccgaa tagaacctct
     7081 cccgctgtgg ctccctgctg ctcattgttg accttatata ctccctgaag tatgttgcca
     7141 gcccggtgtt gtgactgtct tcgcgcaggt caagacccag cggaagctgg ctgaccgagt
     7201 cagccacggc acgggaaata tatccgtacc tctccatctg tgagattacc acattccttc
     7261 tcttcagggc gctctcctca ttgcgtaggg gattgtaggc cacggagttc ttcagcatac
     7321 cgaccagcat tgccgactgc tcgatattaa gcgaatcagg ggtggtattg aaatagatac
     7381 gcgcagccga attgatgccc acggcattat aaatgaagtc aaacacgttg agatacatag
     7441 tgataatctc ttccttcgtg tagctgcgct cgagcttaac agccgtctgc cactccttga
     7501 acttagctat cgccagcctg gaggcacgca caatagccgg atcattggag aggtccctcg
     7561 gcaggaaaag gttcttcgcc agctgctgtg tgatggtact cccacccccg gtgttcttgc
     7621 caagcatgat ggtcttgacc aaaacccttg caagaccacg gaaatcaata cccgaatgtc
     7681 ttttgaaccg gatgtcttca gtggaaataa gcgcatcaat cacataaggt gagatatctt
     7741 cgtaatcagt ccatgtgcgg ttctgtatgt aaaacttgcc aagaaggaca ttgtcttccg
     7801 aatatacctc ggctgcaaga ctgttgtcag gattctcaag ctcctcgaag gaggggatcg
     7861 ggccaagctt gttggcggag atcagtaaaa acaatgtcag caggaacact gccggcagag
     7921 atattataac ccagaaccag ataatgtttt tccggaattt tttatccctc atgataccct
     7981 cattataagg ccgctaattt aatgatttaa tacagaattt ttcttaaaat tttgaagttg
     8041 gcgcccttat tcatttactt tgccctgttt ttaaaaatat tcggatggct gataatctgg
     8101 taatagttga atctcctgcc aaagcaaaga cgatagaaaa atttctcgga aaggattaca
     8161 ccgttgtatc gagcttcggg catatccgtg atctcgttcg aaacaagctg ggtgttgagg
     8221 ttgacaacaa ttttgctccg gtctatgaaa tacctgaaga caagaagaag gttgtagccg
     8281 agcttcgcaa gcttgctgac aaggctacaa cggtatggat tgcttccgat gaagaccgcg
     8341 agggtgaggc aatagcctgg catctgatct ctgttctcgg gcttgacccg gcaaccacgc
     8401 gccggattgt gttccatgag attacgaaag atgccattac acgtgccgtg gaatcgccgc
     8461 ggcaggtaga catgaacctt gtcaatgccc agcaggcacg ccggatactc gacagacttg
     8521 taggctttga gctttcaccc gtgctgtgga aaaaggtaca gccctcactc tcagccggga
     8581 gggttcagtc tgtcgcagtt cgcctgcttg ttgaccgtga acgggaaata ataggtttcg
     8641 tcgctgactc tgccttcagg gtaaccggca cctttgtcgg aacaaacagt gaagggagcg
     8701 atgtgcagct caaggcagag tgccagaaga aattcagcac ggagcaggaa gcgggactct
     8761 tcctggagaa gtgtgacgct tccgcgttca ccgttgaaaa tatagtggtc aagcccggta
     8821 cccgctcacc ggcgcctcca tttaccacct ccaccctcca gcaggaggcc taccgcaagc
     8881 tcggtttctc agtggcacag accatgtcag tggcacagaa actatacgag gccggaaaga
     8941 tcacatacat gagaaccgac tccaccaatc tctcttcact ggcgctcaac accgccaggg
     9001 aagtgattac caatgagtac ggtgcaggct actcgcgcgt caggcagttt aaaacccata
     9061 cgcgcggcgc acaggaggca catgaagcaa tcaggccaac ctactttgac aggaaggagg
     9121 ttaccggcaa caacaatgag aagaagctct atgaactgat ctggagaagg gcgatagcct
     9181 cccagatggc tgatgccagg gtggaaaaga caaccataac cattggcatg tctggttcac
     9241 ccgtcagctt tacggcaaca ggtgaagtca tcctcttcga cggcttcctg aaggtttata
     9301 ccgaatcatt tgatggcaac gt
//
LOCUS       DAOPBK010000100         9314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7005_length_9314_cov_4.147316, whole genome shotgun sequence.
ACCESSION   DAOPBK010000100 DAOPBK010000000
VERSION     DAOPBK010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7005_length_9314_cov_4.147316"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..991
                     /locus_tag="PLA17_07050"
     CDS             134..991
                     /locus_tag="PLA17_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPK85209.1"
                     /translation="MILIADSGSTKTEWREVSDGVAGKSYISTGINPFFVTGEEMIRL
                     FGKELPELKGAGVSRIFFYGTGVSNASKADIVRGALSSFFGTDKLFIGSDLLGAARSL
                     CQNEPGIACIIGTGSNSCYYNGNEIVANVSPLGYILGDEGGGAVIGRKLVAGVLKKQL
                     PGIVIENFFRAYPYTPAEILDNVYNMPFPNRFLGQFTRFIADNIHVPELQAIITSSFD
                     EFIVRNVLSYPEARRYPVHFTGSIAYHFRSFLEDLLVKHRLQPGLFTLTPMENLVKYH
                     IQNSDLLTI"
     gene            988..1830
                     /gene="murQ"
                     /locus_tag="PLA17_07055"
     CDS             988..1830
                     /gene="murQ"
                     /locus_tag="PLA17_07055"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPK85210.1"
                     /translation="MIRQAEKKQDLSITELPSNFEDLDKMTVNELLTNINHEDAKVHV
                     AVRKAIPDIERLINQILVRMQKGGRLFYLGAGTSGRLGVLDASEIPPTFGVPDTVVIG
                     LIAGGETALRKAVESAEDDYTKSWNELEIFNINTKDSVIGIAASGTTPYVIGGLQKAR
                     ENGILTGCITCNPRGPIVDVAEYPIVVVVGPEFVTGSTRLKAGTAQKMVLNMITTTLM
                     IKLGRVKGNKMVNMQLTNKKLIERGTRMIVEELNIQREAARILLMEHGSVKKALEFYR
                     DKYK"
     gene            1832..2557
                     /locus_tag="PLA17_07060"
     CDS             1832..2557
                     /locus_tag="PLA17_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPK85211.1"
                     /translation="MTNDVFSKIGSGLTLSQKIERRIEEAIRQKKLLPGTRLPTEKEL
                     CTQFAVSRTALREALRRLSARGLIDIRKGSGMYISELKIEDAINSLHLFYDLRFNSDL
                     ILQIIEVRRLFEPEVARLAARNRTDEDIRTLQKNQHELRKCNPDNTQLEVDLINRFHM
                     NLSKATGNPIVIISLEPVYSLLPRMRNLIYGNIEGEKEYTLKYQSELLEALKEKDSQK
                     AYEASVALLERNMEIYKKYFKTN"
     gene            2617..4056
                     /locus_tag="PLA17_07065"
     CDS             2617..4056
                     /locus_tag="PLA17_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPK85212.1"
                     /translation="MTCLTVAAQEPDSLFVGIHQAESQYYSRVFDTATLRAVASPVTP
                     ATLKSRKNGEPAFMVYGWHPYWAGNTAYLNYDYSVLTHIAWFSYEVDTATGLYTTLRG
                     WDITPVIDHAHQQGVRVMLTVTNFGSARNTALLTDTVKQWNLINELVYQLKKRNGDGV
                     NFDFETVPASMKSNMASFCRRAVRGIKAELPNAEISLATPAVNWSDAWDLKALAEICD
                     YLIMMGYNYYWSGSSTAGPVAPLRGENYNITRSIQEDYLDAGVPASKLLLGMPWYGYD
                     WPVTGSSRKAGTTGSGSSRTYIATIPMVNAYGKKFDNDTDVPWMSYQKDAQWRQMWFE
                     DSLSLLLKTDFAKKRDLAGVGIWALSYEAGRPELWNGMKAVIEGTVSSDGSPAMPGTG
                     GSGILNIIPNPVKDKALVSCYISRPCTITITIYDQDGRVVEVTEKASLPPGIMTSEID
                     AGKLAPGIYFCVLNTGEGRSVRKFAVAGN"
     gene            4070..5320
                     /locus_tag="PLA17_07070"
     CDS             4070..5320
                     /locus_tag="PLA17_07070"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPK85213.1"
                     /translation="MKKTVLAIIMAITLQSMNGQMQQAIERIAGEYGLMGLSVVAVND
                     GMIAGEWYTGLRDYDRNLPVDENTKFRIASISKFVMTTAMMKLCDQKRLDLEADAGDY
                     LGFRLRNPNHPEKTVTVRMLLLHTGSLNEGTGYDPFLMATYNNVGNPPSFSELFVPGG
                     KYYTEDMWRKEAPGEYYTYCNANFGLAGTIIERITGQRFEEYIQQTLFIPLGINGSYI
                     AEGVTDINNLAVIYGRKEGKWTPGTDDYKGMMSSPRDFSGYVTGTNGAVFSPQGGLRI
                     SAAELARIMILHMNKGKYDGRRIISGKSIRLMHSAQFISTPANSDNEGVRAGNRGLSV
                     QILPESASGTILPEGSHFLGHSGSAYGLVSAFFFDPKEKCGFIFITNGIAGEREAGPS
                     GSFYSFEEDLIRALRDNGFLTCKK"
     gene            5326..5910
                     /locus_tag="PLA17_07075"
     CDS             5326..5910
                     /locus_tag="PLA17_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPK85214.1"
                     /translation="MNAELITAVAIGIGLAASAGFRVFVPMLVAAIAARTGILPLNES
                     FQWLASWSSIIILGTATVVEILAYYIPVVDNLLDTLSTPLAIGAGTLLLTSVLPIDSE
                     LMKWITGAAVGGGSAAVVQSGSALTRLTSTKLTAGLGNPVVATAENVAATGTSILALV
                     IPFFILTLFLLLTILIFTVVRRRLRRSHNKNLSA"
     gene            6040..>9314
                     /gene="purL"
                     /locus_tag="PLA17_07080"
                     /gene_synonym="purI"
     CDS             6040..>9314
                     /gene="purL"
                     /locus_tag="PLA17_07080"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPK85215.1"
                     /translation="MVIFFRTSSGNIIAVRAEGELTRDDTAKLTWLFGNASRLEAETV
                     SGTFIGPRREMITPWSTNAVEITQNMAVSGISRIEEYIPATDENPDHDPMLQRVYHGL
                     DQDIFTLHHEPEPVREIDDIAAYNEQEGLALSAEEIEYLNNLSRNLGRKLTDSEVFGF
                     SQVNSEHCRHKIFNGTFIIDGEERKSSLFDLIKRTTRENRNHVVSAYKDNCAFLDGPV
                     IEQFAPATQDKPDYFRVKYIQSVLTIKAETHNFPTTVEPVNGASTGTGGEIRDRIAGG
                     KGSLPIAGTAVYMTSYPRPDGPREWEKYCEPRKWLYQTPEEILIKASNGASDFGNKFG
                     QPLICGSVLTFEHFTDMKRYGFDKVIMLAGGIGLGKKSDSTKDTPEKGDLIVLLGGDN
                     YRIGMGGGAVSSVDTGAYDSAIELNAVQRANPEMQKRVYNTLRAMAESDHNPVISLHD
                     HGAGGHLNCLSELVEASGGTIDISKLPVGDPTMSAREIIGNESQERMGLVIRSSDIDQ
                     LTSVARRERAPIYVIGEVTGDRQFTFLNPSGGNRPIDLQLSDFFGKPPRTVMNDTTLK
                     SLQEDLTYEPSDPARYLDAVLQLEEVACKEWLTSKVDRSVTGLIAVQQCAGELQLPLN
                     DLGAIALDYRGMNGMATSLGHAPAAGLISAAAGSVLSIAEALTNIVWAPMPDGLKSVS
                     LSANWMWPCKNPGEDARLYEAVQAASDFAVALGINIPTGKDSLSMTQKYPDGPVYSPG
                     TVIITAVAGVEDIKRIVGPVIKNDVTYSLFWIDMSGMRHEPGGSAFSQTIGSLGAMAP
                     GVADAGYFAATFETLQMMIKDDRIIAGHDISAGGMIVTLLEMCFANREGGMALNLTGI
                     GESDSVRLLYSQNPGVIIQVRDKGATEEFLSERGVRFASIGHPVRERKLSIVNGNNHF
                     EFDIDHCRSLWFRSSYLLDRRQCKPSYARERFDNLGDNAIHYDLKNFKGNFRSLDITP
                     DRKATTGVRAAIIREKGVNGDREMAYALWLAGFDVRDVHMTDLITGRETLHDLNMIVF
                     AGGFSNSDVFGSAKGWAGAFRYNEKARIALENFYRREDTLSLGVCNGCQLMAELGLIV
                     PEH"
BASE COUNT         2493 a         2308 c         2495 g         2018 t
ORIGIN      
        1 catctgacaa aaactgataa ttcaatataa ataattacat ttgttatttc tttcggttac
       61 ggaacggaca aaaaaacgta acttgttcct gtaatccgct gagcaaaaat attaaagcct
      121 atttcctaca cagatgatac tgatagcaga cagtggctct acaaagactg aatggagaga
      181 ggtgagcgat ggagttgcag ggaagtcata catctctacc ggaatcaatc cgttttttgt
      241 gacgggtgaa gagatgatcc gtctttttgg caaagagttg ccggaattga agggagcggg
      301 tgtcagccgg atattctttt atggtaccgg ggtaagcaat gcttccaagg ctgatattgt
      361 caggggagca ctctcttcat ttttcggcac cgataagctg tttatcggca gtgacctgct
      421 cggagcagcc aggtcactgt gtcagaatga acccgggata gcatgtatta tcggcacagg
      481 ctccaactcc tgttattata atggtaatga gattgttgcc aatgtatcgc cactgggtta
      541 tattcttggt gatgagggag gcggagctgt cataggaagg aaactggtgg cgggggtgct
      601 caagaagcag cttccgggca tagtgatcga gaactttttc agggcttatc cctacactcc
      661 ggctgagatt cttgacaacg tctacaacat gcctttcccg aacaggttcc tgggacagtt
      721 taccaggttc atcgctgaca atatccatgt accggagctc caggccatta tcacatcaag
      781 cttcgatgag ttcattgtcc gcaatgtgct ttcatatcct gaggcacgca ggtacccggt
      841 tcatttcacg ggaagtatag cttatcattt cagatcgttc ctggaggatt tgctggtaaa
      901 gcacaggcta cagccggggt tgttcacact tactcccatg gaaaacctgg tgaagtacca
      961 tattcagaat tcagatttat taacaatatg atcagacagg ctgagaaaaa acaggacctg
     1021 agcattacgg agttgccgtc aaattttgag gatcttgaca agatgacggt caatgaactg
     1081 cttaccaata tcaaccacga ggatgcaaag gttcatgtag cggtaaggaa agccattcct
     1141 gacatagaga gactgattaa ccagatactt gtcaggatgc agaagggagg ccgcctcttt
     1201 tatcttggtg caggcaccag cggaagactg ggtgtgcttg acgcctctga aataccccct
     1261 acattcggtg ttcccgatac ggtggtgatc ggtctcatcg ccgggggcga aactgcactt
     1321 cgcaaggcag tggagtcagc tgaagatgac tacacgaaat catggaacga gcttgaaata
     1381 ttcaatatca acactaagga ctccgtcata ggcatcgctg cctcggggac caccccctac
     1441 gtgataggcg gcctgcagaa ggcaagagag aatggaatcc tgacgggatg catcacatgt
     1501 aaccccaggg ggcccatagt ggatgtggcc gaatacccca tagtggtagt ggtgggtcct
     1561 gagttcgtta ccggaagcac ccggctgaag gccggaacag cgcagaaaat ggtgctgaac
     1621 atgatcacaa ccacccttat gattaagctt ggaagggtca agggaaacaa gatggtcaac
     1681 atgcaactga ccaacaagaa actgattgaa aggggaacga ggatgatagt ggaggagctt
     1741 aatattcaaa gggaagcagc acgcatactc cttatggagc atggatcggt aaaaaaagcc
     1801 ctggagtttt acagggataa atataagtaa aatgactaat gatgtcttca gtaaaatcgg
     1861 ttcgggactg acactcagtc agaagattga gaggcggatc gaagaagcaa tacgacagaa
     1921 gaagctgctg cccggcacca ggctgccgac cgaaaaagag ctctgcactc agtttgccgt
     1981 cagccggaca gccctgaggg aagccctcag gcgtctcagt gcacgcggac tcatcgacat
     2041 ccgtaaggga agcggaatgt atatctctga gctcaaaatc gaggatgcca tcaattcact
     2101 acatctgttc tatgacctca ggttcaactc tgacctcatc ctgcagataa ttgaggttcg
     2161 caggctcttc gagccggaag tggccaggct ggcggcacgc aaccgtacgg atgaggacat
     2221 ccgcaccctg cagaaaaacc agcatgaact gaggaaatgc aatcctgaca acacacagct
     2281 tgaagttgac ctgatcaacc ggttccacat gaacctgtca aaagccacag gcaacccgat
     2341 agtgatcata agtctggaac cagtatattc cctcctgccc cggatgagga acctcatcta
     2401 cggcaacatc gagggtgaga aggagtatac cctgaaatac cagagtgaac tgctggaggc
     2461 gctgaaggag aaagactccc agaaggctta tgaggcttca gtagctctgc tggaacggaa
     2521 catggagatc tacaaaaagt actttaaaac caactgatac acctctcaat aatctgcata
     2581 ccgcgcagga taacatttgc agcagtaatt ggcttgttga cctgccttac ggttgccgcc
     2641 caggaacccg acagcctttt tgtaggtatt catcaggccg agagtcaata ctattccagg
     2701 gtttttgaca ctgccactct ccgcgcagtt gcctctcctg tgacaccggc gacactcaag
     2761 agcaggaaaa acggggagcc cgcattcatg gtttatggct ggcacccata ctgggcaggc
     2821 aataccgcat acctgaacta tgactattca gtactgaccc atattgcctg gttcagttac
     2881 gaggtagaca ctgcaaccgg attgtataca acgctgagag ggtgggatat caccccggtg
     2941 atagatcatg cacaccagca gggtgtcagg gtgatgctca cagttaccaa cttcggctct
     3001 gccaggaaca ctgccctgct cactgatacc gtgaaacagt ggaaccttat aaatgagctg
     3061 gtataccagc tgaagaagcg caacggcgat ggcgtgaatt ttgattttga aaccgtacct
     3121 gcttccatga agtccaatat ggcaagcttt tgcaggaggg ctgtcagagg catcaaggct
     3181 gaactgccaa atgccgagat atctctggca acacccgcgg taaactggtc tgacgcatgg
     3241 gaccttaaag cgctcgcgga gatctgtgac tacctgatca tgatgggcta caactattac
     3301 tggagcggtt cctccactgc aggccccgtg gctccgctga gaggagaaaa ctacaacatc
     3361 accaggagca tacaggaaga ctacctggat gccggggtcc ctgcctcaaa actactgctg
     3421 ggaatgccat ggtatggcta tgactggcca gtaaccggca gctccagaaa ggccggaacc
     3481 accgggagtg gctcatcacg gacatatatt gccaccatac caatggttaa cgcttacgga
     3541 aaaaaatttg acaacgatac agatgtgccg tggatgagtt accagaagga cgcgcagtgg
     3601 agacagatgt ggttcgaaga cagtctcagc ctgctcctga agacagactt cgcaaaaaaa
     3661 agggacctgg ccggagtggg aatatgggcg ctgagctacg aggcaggaag acctgagttg
     3721 tggaacggca tgaaggcagt gattgaagga acagtctctt ctgatggcag cccggcaatg
     3781 ccgggaacgg gaggcagcgg gatactgaac ataatcccga atccggtaaa ggataaagcg
     3841 ctggtcagtt gttatatctc ccggccatgc accatcacca tcacaattta tgaccaggac
     3901 ggaagagttg tggaagtgac ggaaaaggct tccctcccgc cgggcataat gacatcagaa
     3961 attgatgcag gaaaactggc acccggtata tatttctgtg tgcttaacac cggcgaaggc
     4021 aggtctgtac gaaaatttgc agttgccgga aactaaataa tataacaaaa tgaaaaaaac
     4081 agtgcttgcc attatcatgg ctatcactct gcagtcaatg aacggacaga tgcaacaggc
     4141 aattgaaaga attgcaggag aatacggcct gatgggacta tctgtggttg ctgtgaatga
     4201 cggcatgatc gcgggtgagt ggtacacagg cctgagggac tatgaccgca acctcccggt
     4261 ggacgaaaat accaaattcc ggatcgcctc catctccaag ttcgtgatga caacggctat
     4321 gatgaaactt tgcgatcaga aaaggctgga tcttgaagct gatgccggtg actaccttgg
     4381 attcaggtta aggaatccca accatcctga aaagaccgtg actgtgagga tgctgttgct
     4441 ccataccgga tcactgaatg aggggaccgg gtatgatccg ttcctgatgg caacctacaa
     4501 caatgtagga aatccaccct cattcagtga gctgtttgta ccaggaggaa agtattatac
     4561 tgaagacatg tggagaaagg aagcgcccgg ggaatattac acatactgca acgcgaactt
     4621 cgggctggcc ggcacaatca tagagaggat aacagggcag cgttttgagg agtatatcca
     4681 gcagactctc tttattccgc tgggcatcaa cggatcatat atcgccgaag gggttactga
     4741 catcaacaac cttgcggtaa tttatggcag gaaagagggc aaatggaccc cgggcactga
     4801 tgactacaaa gggatgatgt cgtcaccgcg cgatttttcg ggttatgtca cgggaaccaa
     4861 cggtgcggtc ttttcgccac agggcggcct gaggatctct gccgcagagc tggcacgtat
     4921 tatgatactg cacatgaata aaggaaaata tgacggcagg cgtatcattt ccgggaagag
     4981 tatcaggctg atgcacagtg cacaattcat atccacaccc gccaacagtg acaatgaagg
     5041 ggtacgggca ggcaatcgcg gactgagtgt tcagatactg cctgaatcag catccggcac
     5101 catcctccct gaaggttctc acttcctcgg acactcgggg tcagcttacg gtctggtctc
     5161 agcctttttc ttcgatccga aagaaaagtg cggattcatc tttatcacca acggcattgc
     5221 cggggagcgg gaagcaggac catcgggctc gttttacagc ttcgaggagg acctgatcag
     5281 ggcgcttcgc gacaacggat tcctgacatg taaaaaataa ttgcaatgaa tgctgaactt
     5341 atcactgctg ttgcaatagg catcggactg gcggccagcg ccggcttcag ggtgttcgtg
     5401 ccgatgctgg ttgctgccat cgcagccaga accggcatac tgccactgaa cgaaagtttt
     5461 cagtggctgg catcatggtc ttcgattatc attctcggaa cagcaacggt ggttgagata
     5521 ctggcctact acatacctgt ggttgacaat cttcttgata ctctctccac gcctctggcg
     5581 ataggagccg ggaccctcct gctcacctcg gtgcttccta ttgacagcga gctgatgaag
     5641 tggatcaccg gcgctgccgt gggtggtggc agcgctgccg tggtacagag tggcagtgcc
     5701 ctgacaaggc tcacctcaac aaaactgacg gccggccttg gcaacccggt agtggcaacg
     5761 gcagagaacg tggctgccac aggaacctca atccttgctc tggtaatacc cttttttatc
     5821 ctgaccctct ttctgctgct cacaatactg atcttcaccg tagtcagaag gaggctgcgc
     5881 aggtcacaca ataaaaattt aagcgcctga agatattata tgacacaagg atgccacagt
     5941 gctcctccgg tttggttgtt ttattatctt tggcaacaac tacagtagcg gcgcctcagc
     6001 cgttacttcg ctattcataa gcattctatg aactctggta ttgtaatttt cttcagaact
     6061 tcctccggga atattattgc tgtcagggca gaaggagaac tcacccgtga tgacacggca
     6121 aagctcacat ggctgttcgg taatgccagc cgcctggaag cggagaccgt cagtggcaca
     6181 ttcatcggac cccggcgcga gatgataacc ccctggagca ctaacgccgt ggagataaca
     6241 cagaacatgg cagtgtcagg catctcaaga atcgaggagt acattcccgc cacggatgaa
     6301 aatccggatc acgatcccat gcttcagagg gtttatcacg gacttgacca ggatatcttt
     6361 accctgcacc acgagccgga accggtacgg gagattgatg acatagctgc ctacaatgaa
     6421 caggaaggtc ttgcgctgag tgctgaggag atcgaatacc ttaataacct gagccgcaac
     6481 ctgggaagaa aacttacaga cagtgaagta ttcgggttct ctcaggtcaa ctccgagcac
     6541 tgccggcaca agatcttcaa cggcacattc atcatagacg gcgaagagcg caaatcgtca
     6601 ctctttgacc tgatcaagcg taccaccagg gagaaccgga atcacgtcgt ttcggcatat
     6661 aaagacaact gcgcctttct tgacgggcct gtcatcgaac agttcgcccc ggcaacccag
     6721 gacaaacctg attatttccg ggtaaaatac attcagtcgg tgctcaccat caaggctgaa
     6781 acccataatt tccccactac cgttgagccc gttaacgggg cctccaccgg taccggcgga
     6841 gagatacgtg accgtatcgc gggcggcaag ggctcactcc ccattgccgg cacagcagtc
     6901 tatatgacat cctatcccag accagacgga ccccgcgagt gggaaaaata ctgtgaaccg
     6961 cgcaaatggc tttaccagac acctgaagag atactgatca aggcttccaa tggcgcgagc
     7021 gacttcggga acaagttcgg tcagccgctt atctgcggct cggtgctgac cttcgaacac
     7081 ttcactgaca tgaagcgcta cggctttgac aaggtgataa tgcttgcggg cggaatcggc
     7141 ctcgggaaaa aatccgacag caccaaggat acacctgaaa agggtgacct gatcgtgctc
     7201 ctcggcggcg acaattacag gatcggaatg ggcggagggg ctgtctcttc tgttgatacc
     7261 ggagcctatg acagcgccat agagctcaat gcagtacagc gtgccaatcc cgagatgcag
     7321 aagagggttt acaataccct cagggccatg gcagaaagtg accataaccc ggtgatatca
     7381 cttcatgacc acggcgccgg cggccatctc aattgtcttt ccgaactggt agaagcaagc
     7441 ggtggaacca ttgacatcag caaattgccg gtgggtgatc ctaccatgtc agccagagag
     7501 atcattggca acgaatcaca ggaacggatg ggccttgtaa tacgaagcag cgatatagac
     7561 cagctgactt cagtggcccg ccgcgaacgg gctccaatat atgtcatcgg ggaggtgaca
     7621 ggtgatcgcc agttcacctt ccttaatccg tccggtggca acagacccat tgacctgcag
     7681 ctctccgact tcttcggaaa gcctccgcgg acggtcatga atgacaccac cctgaagagc
     7741 ctgcaggaag atctcaccta tgaaccttct gacccggcaa gataccttga tgcagtgctg
     7801 cagcttgagg aagtggcatg caaggagtgg ctgacaagca aggttgaccg ttccgtgacc
     7861 ggtctgattg ctgtgcagca gtgcgccggc gaactgcaac tgcccctcaa tgatcttggg
     7921 gccatagcgc ttgattaccg gggcatgaac ggcatggcta cctcgctcgg tcatgctcct
     7981 gcagccggcc tcatcagcgc cgctgccgga tcagtgctct cgatagccga agcacttacc
     8041 aatatcgtct gggctccaat gcctgacgga ctgaagagtg tatccctgtc ggccaactgg
     8101 atgtggccat gcaaaaaccc gggagaggat gcaagactat acgaggcagt acaggcggcc
     8161 agcgacttcg cggtagcgct tggaataaat atcccaaccg gaaaggacag cctctcaatg
     8221 acccagaaat accctgacgg acctgtctac agtcccggca ccgtcatcat caccgctgta
     8281 gcgggggtag aggatataaa gcgcatcgtt ggtccggtga taaaaaatga tgtgacatat
     8341 tcactcttct ggattgatat gagcggcatg aggcacgagc ctggaggttc ggcgttcagc
     8401 cagaccatcg gcagtctggg agccatggca cccggagtgg cagacgcggg atacttcgca
     8461 gccacattcg agacactgca aatgatgatt aaagatgaca ggatcattgc tggtcatgat
     8521 atctctgcag ggggaatgat tgtcaccctc ctggagatgt gttttgcaaa ccgtgagggg
     8581 ggaatggcgc tgaacctgac cgggatcgga gagagtgaca gcgtcaggct gctctacagc
     8641 cagaaccccg gtgttatcat acaggtcagg gacaagggtg cgacagagga gttcctcagt
     8701 gaaagaggtg tgcgtttcgc ctccatcgga catcctgtaa gagaaagaaa actgtcaata
     8761 gtcaacggca acaaccattt tgagtttgac attgaccatt gccgctcgct atggttcagg
     8821 agctcatacc ttcttgatcg ccggcagtgt aaaccgtcat atgccaggga gcggtttgac
     8881 aacctgggag acaatgcaat tcactatgac ctgaagaact ttaaaggcaa cttccggtca
     8941 cttgacatca cccctgacag gaaggcaacc acaggagtca gggctgcaat catcagggag
     9001 aaaggggtga acggtgaccg cgagatggct tatgctctct ggctggcagg atttgacgta
     9061 agggacgtgc atatgaccga cctcatcaca ggacgtgaga cactgcacga tctcaacatg
     9121 attgtctttg ccggcggctt ctcaaactcc gatgttttcg gttcagccaa agggtgggcc
     9181 ggggcgttca ggtacaacga gaaggcccgg atagcgcttg agaatttcta caggagagaa
     9241 gatacactct cactgggcgt ctgcaacgga tgccagttga tggcagaact cgggctgatt
     9301 gtgccggaac acaa
//
LOCUS       DAOPBK010000101         9313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7006_length_9313_cov_5.084576, whole genome shotgun sequence.
ACCESSION   DAOPBK010000101 DAOPBK010000000
VERSION     DAOPBK010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7006_length_9313_cov_5.084576"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLA17_07085"
     CDS             <1..446
                     /locus_tag="PLA17_07085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK85216.1"
                     /translation="HDNVVLIQEDNLPKKLILKIDTARMTRIVNNLFRNALDNTLSGF
                     IKVGCTWYDDTITFYVKDSGQGYPRSSNLLLSDNPEILHSESQDTYSAMNLILARNLI
                     LAMGGRIRVENNDAGGTSVFVSLPARESSALKILTGTSSENKIAI"
     gene            complement(565..2517)
                     /locus_tag="PLA17_07090"
     CDS             complement(565..2517)
                     /locus_tag="PLA17_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK85217.1"
                     /translation="MSTGRKILTLMFLVLIHCSVTGQNFHTRSNRALKYYDLGKRDYD
                     LLYYDRAETNLLHAVKEDDKFYEAHLILGQLYSDTGNWEKAVTHYRAAVATDSLFFVP
                     ALYSLGRAELRTGRYYDALLHLDAFLKQPGISSRLRGEAEKMLASCRFALSFPESLFQ
                     TEPVNVGDSVNTVLDEYWPSVTGDGQQLIFTREVRRATGYGKDYNRDRQEDFYISRLT
                     ADGYWGTARNAGAPLNTAGNEGAQSISSDGRYMYFTACDRNDGQGRCDIYFSSYDGNR
                     WSPGINIGPPVNTTYWESQPSISPNGRMLFFVSNRPGGMGGMDIWYSLRGSDGKWGKP
                     VNPGKVINTAGDEISPFIYFNGKTLYFSSNGRESFGGHDIYVTTMNRDSTWTVPENLG
                     PPVNTPADETGLVIESSGRRAYFSSDREKGKGKDIYYLDLPQRIQPEQVLYFQGTVID
                     KASGQPIVAKYELSDLTNGVNVITSLTDKRGGFFVCLPQGSSYGMNVTADGYMIYSEN
                     FNFVEGYTSAEPYRKTIALNRIREGEFMRMYNVFYDTDSWELLESSRPELEQLLEFLQ
                     VNHTVVIEIGGHTDSDGSDEYNQRLSERRANSVREFLIKRGIDGKRIFSHGYGETHPV
                     ADNLTPAGKRLNRRTEITILSEGVTR"
     gene            complement(2518..3144)
                     /locus_tag="PLA17_07095"
     CDS             complement(2518..3144)
                     /locus_tag="PLA17_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPK85218.1"
                     /translation="MRKRETDNSGLLLPLVEEFYSIQGEGVNTGRAAYFIRVGGCDIC
                     CSWCDTRHSWNPELHPMVSIVEIAGNVTAAGADSVVVTGGEPLMWNMEPLCRLLRSKG
                     IKTFLETSGTHPLTGKWDWITLSPKKDSPPLSGILEMADELKVVIHDGTDFEAAEMYS
                     RNVRPACQLLLQPEWSRYREMIKAITEYVKKNSRWRVSLQAQKFMHIP"
     gene            complement(3141..4247)
                     /locus_tag="PLA17_07100"
     CDS             complement(3141..4247)
                     /locus_tag="PLA17_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPK85219.1"
                     /translation="MKILTRIGALALVVLTLASAGSSRQKPRNLILFIGDGMGVSHVY
                     AAITASGNTMTFPSFPVTGFSRTHSANNYSTDSAAGGTALATGEKTNNLMVAMRPDST
                     ILVTLLEHAKAAGMATGIVCTSSLTDATPADFATHAPLRYEYRSIAKQYINGAADIFI
                     GGGRRYFVPTPDSTGKINHEEDVVSGLSRAGYDVTYDLESFIASKSDRIAGLMADGDI
                     PMISDGRDTQFLARATAKAIEVLSRNRAGFILMVEGSRIDDAGHGNNTSMVIDEVIDM
                     DRAVAVAYDFAKRDGKTLIVVTADHETGGMSITGGNLAKREVKAEFSFTGHSGVMVPV
                     FAFGPGAVEFTGIQENTDLFHDFFRLLSLDRKRR"
     gene            complement(4260..5996)
                     /locus_tag="PLA17_07105"
     CDS             complement(4260..5996)
                     /locus_tag="PLA17_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPK85220.1"
                     /translation="MIKTTTRNLALILLAALLTLACTRKEVRLEIYATTDLHGMLLPF
                     DNTEGKSTDRSLANLARVVESGKKENIVLLDNGDILQGDPLAYYYNFIDTATTHIIAD
                     ILNHLGYDAATVGNHDIETGHAVYDRVRSEYNFPMLAANAIEIETGEPYFEPYTVIKK
                     REMKLVVFGLITPSVPRWLPESLYSGIRFENMVATARKWMPAIQAENPDLVIGLFHSG
                     MGDGDNGGDDENSSLAVAMNVPGFDVIFCGHDHRKDVREVTDSNGDKVLVLNGGSRAE
                     ALMNVSVSFDRRGAGRNIPLLEGRITEMNTLPESSGFLERYRGAMERVREYTSEIIGR
                     SETTVGTRDAFFGPSAFTDLIHRMQLDITGAQISLAAPLSFDQSISSGDLRIRDMYRL
                     YRFENFLYTVRMTGGEIDRHLEHSAGHWFRTMEKASDYMLNYRTDDDGKPVIVNGTAR
                     LQYPSYNFDSAMGIRYTVDVSKPAGARVNITALSDGSPFSHSASYTVAVNSYRANGGG
                     GHFTAAGIAGKELERRIISATERDLRFYMTEWIREKGTISPVPLSEWKIVPVDWAADA
                     KKREMRMLFGNN"
     gene            complement(6001..7299)
                     /locus_tag="PLA17_07110"
     CDS             complement(6001..7299)
                     /locus_tag="PLA17_07110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPK85221.1"
                     /translation="MKPLLNALVTALILISFQAVAQEGITKYIPSAKPERIVLNITGD
                     QGTTMAVTWRTDTTVSESVARISRNISGVLRADSAMAVTGKSEDITADGVTARFHSVI
                     FTGLVPGTGYSYCVGSGPDASEWFTFKTAEAGAAPFSFLWFGDSQVGPISSYARVIRQ
                     AYRSVPEAAFMIFTGDLVDGGYAGNTLDDEWGDWHRAAGFIASEVPVAATPGNHEFYN
                     PHDRQKRDLNMYWRPGFTLPENGPAGLEETAYSFDYQGVRFIMVSSDQMLRDEAIARS
                     QTEWVESLLKNNPCKWTVMAFHHPFFSTAARRNNKVVRENLKPLVDRYGVDLVLNGHD
                     HTYARGVVMPDGAEQRGRRAGTVYVVSVSGSKQYSQDAAPWWEAGYTNTQLWQAITID
                     GNRLIYKAFDASGALADQFMLTKMKNGSNRIGLPPEVKTR"
     gene            7421..8599
                     /locus_tag="PLA17_07115"
     CDS             7421..8599
                     /locus_tag="PLA17_07115"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPK85222.1"
                     /translation="MKALSGLDVVTLGLPAGLRGKRTGLLCHSSSISSDFRHITEIFG
                     QSKECRLVSLFGPQHGLSGQTQDNMIEWEGTTDPLLGIPVYSLYGEHRKPTDAMLEGI
                     EVFVIDLQDVGARLYTYIWTVKLCMEACAEAGIPVYILDRPNPIGRIQPDGPVLSEEY
                     FTFVGGASIPLCHRMTMCEMAIWVKEKHIPGCDLHVIRAEGWRRNMMYHETGLPWVLP
                     SPNMPTPQTAAVYPGTVLAEALNISEARGTVIPFELMGAPWIDGRRLLEEMRSRKLPG
                     CQFRMHDFIPTFHKYAGEYCRGIQIHVTDPALFRPVATAMHLFDAIIRTSPEGSLKFN
                     PPPYEYEYRLMPFDILSGDSRMREVLTGGLSLKDETERWEAQTETFMADFRQIALYPD
                     "
     gene            8601..>9313
                     /locus_tag="PLA17_07120"
     CDS             8601..>9313
                     /locus_tag="PLA17_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPK85223.1"
                     /translation="MITAGNIIALSIIIFLASVVRGFTGFGLALVAVPLIQFIFPVSD
                     TAVFIAVINMIFSILYYRRSRELVREQPLGRMALFTGVGVAAGVLILKYINPAYIQLT
                     WGILIILIVTALVRGANLRISSDAPAMTLSGLFGGLLAGATGITGPPVAIILSAMKTP
                     KEKFNAVISLFILFAVSYALLFYLISGLIRRDTVLLALCSVPALIAGLRTGDILVSRI
                     SQKTFTNVIYIVLIIMGIIT"
BASE COUNT         2176 a         2483 c         2237 g         2417 t
ORIGIN      
        1 gacacgacaa tgtggttctc atccaggagg acaaccttcc caaaaagctg atactgaaaa
       61 ttgatacggc aagaatgacc aggattgtaa acaatctgtt caggaatgca cttgacaata
      121 ctttatcagg gtttattaaa gtggggtgca cctggtatga tgatacaatt accttctatg
      181 taaaagattc tggccagggt tatcccagaa gcagcaatct gctgctttcc gacaatcctg
      241 agatactaca ttccgaaagt caggatacct attccgctat gaatttaatc ctggccagaa
      301 atcttattct tgcaatgggt ggcaggatca gggttgaaaa caacgatgcc ggaggcacct
      361 ctgtcttcgt ttccttacct gcaagggaga gctctgctct gaaaatactt accggtacct
      421 cttcggagaa taagatagcc atctgattct ccggggcttt aaactgtctg ttaagagcgg
      481 ttgggagggt agctaaaagg caccctcctt tactggtttg tatctgttta ttgattgtgg
      541 ataatccttc tgtcttccgg cgtgtcatct ggtgacacct tccgacagta tagttatctc
      601 tgtccgcctg ttaagacgct tgcccgcagg ggtgagattg tctgccacag gatgggtttc
      661 tccgtaaccg tgtgaaaaga tacgtttccc gtcaataccc cgctttataa ggaattccct
      721 gactgaattg gctcttctct cagaaagccg ctggttatac tcatcacttc cgtcagagtc
      781 agtatgtccg ccgatctcaa ttactacagt gtggtttacc tgcaggaatt caagcagctg
      841 ctcaagttca ggccgcgatg attcaagcaa ttcccatgag tcagtgtcat agaacacgtt
      901 gtacatccgc atgaattctc cctcacgaat cctgttcagg gcaatggtct tcctgtaagg
      961 ctccgcagat gtatagccct caacgaaatt gaaattctca gaatagatca tgtaaccgtc
     1021 tgccgtgaca ttcatcccgt aactgcttcc ctgcggaaga cagacgaaga atcctcccct
     1081 cttgtcagtc agtgaagtta ttacattaac accgtttgta aggtcagaca gttcatactt
     1141 tgcaacaatg ggttgccctg acgccttgtc aataaccgtc ccctggaagt acagaacctg
     1201 ttcgggctga atacgctgtg ggagatcaag gtaatagata tccttccctt tccccttttc
     1261 acggtctgag gagaagtagg cccttcggcc cgaagactcg ataacgaggc ctgtttcatc
     1321 agcaggggta tttaccggcg gacccaggtt ttcagggact gtccaggtgg aatcacggtt
     1381 catggtggtg acatagatat catgtccgcc aaatgattcc ctgccgttgg atgagaagta
     1441 gagcgttttt ccgttgaagt agatgaaggg tgaaatctcg tcgccggcag tgtttatcac
     1501 ctttcccggg ttgaccggtt tgccccattt cccgtcactg ccacgaagtg aataccatat
     1561 atccattccc cccatccctc cgggcctgtt gcttacgaag aaaagcatcc ttccgttggg
     1621 gcttatagat ggctgtgact cccagtatgt tgtgttgaca ggaggcccga tattaatccc
     1681 gggcgaccac ctgttgccat catatgatga gaagtagatg tcacatctac cctgtccgtc
     1741 atttctgtca caggcagtaa agtacatata ccttccgtca gaggagatgg actgtgctcc
     1801 ttcattgcct gcggtgttca gcggagctcc ggcattcctg gccgttcccc agtatccgtc
     1861 ggcagtcagc ctgctgatat aaaagtcctc ctgcctgtcg cgattatagt ctttgccata
     1921 ccccgtggcc cttctcactt ctcgtgtgaa tatcaactgc tggccgtcac ctgtcactga
     1981 aggccagtac tcgtcgagga cagtattgac cgagtcgccg acgttgaccg gctcggtttg
     2041 aaagagagat tccgggaacg aaagggcaaa acgacagctg gcaagcatct tttcagcttc
     2101 accgcgtaac ctggaggaga ttcctggttg tttcagaaaa gcgtcgaggt gaagcaaagc
     2161 atcatagtat cttccggttc gcaactcagc cctgccaagg ctgtagagtg ccggtacaaa
     2221 gaagagggag tcggtggcaa cagcagcgcg gtagtgggtc actgcctttt cccagttacc
     2281 tgtgtcagaa tagagctggc cgagaatcag gtgagcctca tagaatttgt catcctcttt
     2341 caccgcatga agaagatttg tctctgccct gtcgtaataa agaaggtcgt aatccctttt
     2401 gcccaggtca tagtacttca gcgccctgtt tgaacgtgta tgaaagttct gtccggtaac
     2461 tgaacagtgg atcagaacca ggaacataag ggtcagtatt tttcttcctg tactcattca
     2521 ggggatatgc atgaattttt gtgcctgcag tgagaccctc caccggctgt ttttcttcac
     2581 atattccgta attgccttta tcatctcccg gtacctgctc cattccggtt gcagaagaag
     2641 ctggcaggcc ggtctcacat tccggctgta catctctgcc gcctcaaaat cagtgccatc
     2701 atgaattacc accttcagct catctgccat ttccaggatt ccggagaggg gcggcgaatc
     2761 cttctttggc gagagggtta tccagtccca cttgcctgta aggggatgtg ttcccgaagt
     2821 ctcaaggaat gttttgattc cttttgacct gagcaggcga cacaatggct ccatattcca
     2881 catcagcggc tcgccgccgg taacaaccac cgaatcagcc ccggcagccg tcacgtttcc
     2941 ggcaatctcc acaatggaga ccatcgggtg gagttcgggg ttccatgaat gcctggtgtc
     3001 acaccagctg cagcatatat cacaccctcc aacccttatg aagtaagctg ccctgccggt
     3061 attgacaccc tctccctgga tagaatagaa ctcctccacc agggggagta gcaggccgct
     3121 gttatctgtc tccctcttcc tcatctgcgt ttcctgtcaa gagaaagcag cctgaagaag
     3181 tcatgaaaga ggtcagtgtt ttcctgtatg ccagtgaatt ccacagctcc cggcccaaaa
     3241 gcaaagaccg gcaccatgac acctgaatgc ccggtgaaac tgaactcagc tttgacctcc
     3301 ctctttgcaa gatttccccc ggtgatactc atacctcctg tctcatggtc agcagtcacc
     3361 acaatcagtg ttttgccatc cctcttcgca aaatcatagg caacggcaac agccctgtcc
     3421 atatcaatga cctcatcaat caccattgat gtattattgc catgaccggc atcatcaatc
     3481 ctcgaacctt cgaccatgag gataaacccc gcgcgattcc gcgaaagcac ctctatagcc
     3541 ttggctgttg ccctggcaag gaactgagta tctctcccat cggatatcat gggaatgtca
     3601 ccatcagcca tcagccccgc aatccggtct gacttcgagg cgatgaacga ctcaagatca
     3661 tatgtaacgt cataacctgc ccgggaaagc cctgatacca catcctcctc atggttgatc
     3721 ttcccggttg aatccggggt tgggacgaaa tacctcctgc ctcccccgat gaaaatgtca
     3781 gctgccccgt tgatgtactg cttcgctatt gaacgatatt catacctcag gggggcatga
     3841 gtggcaaaat cagccggggt ggcgtcagtc aacgaactgg tacagacaat gccggtagcc
     3901 attccggccg ctttggcatg ctcaagaagg gtaaccagta ttgtgctgtc ggggcgcatc
     3961 gctaccatca ggttgttggt cttctcacct gtggcaagcg ctgttccgcc ggcggctgag
     4021 tcagttgaat agttgttggc cgagtgagtc ctgctgaagc ctgtcaccgg gaaggaagga
     4081 aaggtcatgg tgtttcctga ggccgttatg gcggcataga catgtgagac tcccattccg
     4141 tcgcctataa acaatataag gtttcgtggc ttctgtctgg aactgcctgc tgaggcaagc
     4201 gtcagaacaa caagggctaa agctccaatt ctggtaagta tcttcatgaa aatctattgt
     4261 cagttattgc caaaaagcat tctcatttcc cgttttttcg catcagctgc ccaatcaacc
     4321 ggaacaatct tccactcact gagcggaaca ggagagattg tgcccttctc ccttatccat
     4381 tcagtcatgt aaaaccgcaa atcacgttcc gtcgctgaga taatccttct ctccagttcc
     4441 ttgccggcaa tacccgctgc cgtaaagtgc cctccgcccc cgttggcccg gtatgagttc
     4501 acggctactg tataactggc gctgtggctg aacggagacc cgtcactgag tgcagtgata
     4561 ttgacccttg ccccggcggg tttgctcacg tctactgtgt acctgatgcc catggcagaa
     4621 tcaaaattgt atgacggata ttgcagcctg gcagtcccgt ttacgatgac cggcttgccg
     4681 tcatcatctg tgcggtagtt aagcatgtag tcactcgcct tctccattgt ccggaaccaa
     4741 tgacccgctg aatgttcaag atggcggtct atctctcctc ccgtcatcct gacggtataa
     4801 aggaaattct caaaccggta tagcctgtac atgtccctga tcctcagatc tccactggag
     4861 atgctctggt caaatgagag cggggcagcc aatgatatct gagctcctgt gatatcaagc
     4921 tgcatgcggt gtatcaggtc ggtgaaggcc gagggaccaa aaaaggcatc gcgcgtgcct
     4981 acagtggttt cagacctgcc gatgatctcg gaagtatact ccctgaccct ctccattgct
     5041 ccgcggtacc tctcgaggaa gccggatgac tccggaaggg tattcatctc tgttattctg
     5101 ccttcaagga gcggtatgtt ccttcctgca cctctcctgt caaatgagac actgacattc
     5161 atcagtgcct ctgccctgct gccaccgttg agaacaagca ccttatcacc gttgctgtcc
     5221 gtcacttccc tgacatcctt cctgtggtca tgcccgcaga agataacgtc aaaaccggga
     5281 acgttcattg caactgccag agagctgttc tcatcatctc ctccattatc tccgtcgccc
     5341 attcctgaat gaaacagccc gataaccagg tcaggatttt cagcctgaat ggcaggcatc
     5401 catttcctgg cagtggcaac catgttctcg aaccttatac cactgtaaag tgactccggc
     5461 agccagcgcg gaacagacgg ggttataagc ccgaagacca caagcttcat ttccctcttt
     5521 tttatgacgg tataaggctc gaaataaggt tctccggttt ctatctctat ggcatttgct
     5581 gccagcatcg ggaagttgta ttcgcttctg acgcggtcat acacagcatg gcccgtttct
     5641 atgtcatggt tgcccacggt ggctgcatca tagcccagat ggttgagtat gtcagctatt
     5701 atatgggtag tggcagtatc tatgaagtta taatagtatg ccagcggatc accctgcaga
     5761 atgtcaccgt tgtcgagcag aacaatattc tcctttttgc ccgattcaac cacccgggca
     5821 aggttggcaa gcgaccggtc agtggacttg ccctctgtat tgtcaaatgg gagcagcatg
     5881 ccgtgcaggt cagtggtggc atatatttcg agcctgacct cctttcttgt gcatgcaagc
     5941 gtaagcaggg cagcaagaag tattaatgcc agattccggg tagttgtctt gatcatggta
     6001 ttatcttgtt tttacttcag gaggcagccc tatccggtta cttccatttt tcattttggt
     6061 gagcataaac tggtcggcaa gggcaccgga tgcatcaaat gccttatata tcagccggtt
     6121 accatcaatg gtaattgcct gccataactg ggtgttggta taccctgctt cccaccacgg
     6181 tgcggcatcc tgactgtact gtttggagcc gctgaccgag actacataaa cggttccggc
     6241 cctcctgccc ctctgttctg ctccgtcagg catcacgact cccctggcat atgtatggtc
     6301 atggccgttc agtaccaggt caaccccata cctgtcaacc agcggtttaa ggttttccct
     6361 gacaaccttg ttgtttctcc tggcagcagt tgaaaagaag ggatggtgaa atgccattac
     6421 ggtccatttg catggattat tcttcagaag gctttcaacc cattcggtct gactgcgggc
     6481 aatggcttca tctctcagca tctggtcgct gctgaccatt atgaaccgca caccctggta
     6541 gtcgaaactg taggctgtct cctccaatcc ggcgggtccg ttttccggga gagtgaaacc
     6601 cggtctccag tacatattta agtcacgctt ctgtctgtca tgcgggttgt agaattcatg
     6661 gttcccgggg gttgctgcca ccggcacctc cgatgcgatg aatcctgcag cccggtgcca
     6721 gtcaccccac tcatcgtcaa gagtattgcc ggcatatcca ccgtcaacca ggtcaccggt
     6781 aaatatcatg aatgctgctt caggcactga gcggtaagct tgtctgatca ccctcgcgta
     6841 ggaagaaatc ggcccaacct gtgaatctcc gaaccagaga aaggagaacg gggctgcacc
     6901 tgcttcagca gttttgaagg tgaaccactc gctggcatct gggcctgaac cgacgcagta
     6961 agaatatccg gttccgggaa ccagcccggt aaatatcacc gaatggaacc gggcagtcac
     7021 tccatcagca gtgatatcct ccgattttcc tgttacggcc atggcagagt cagccctgag
     7081 cacaccagat atgttgcggg aaatccttgc cacactctcg cttactgtgg tatccgttct
     7141 ccaggtcaca gccatggttg ttccctgatc tccggtaata ttgagtacaa tcctttcagg
     7201 ttttgcagag ggaatatatt ttgtaattcc ctcctgtgcc actgcctgaa atgaaatcaa
     7261 tatcagagcg gtcacgagag cattcagaag aggcttcata attggtttta ttacattaac
     7321 aattctccgt gacgggaggt tgtaaatata ttgataattt ccatgcagag cggcggaccg
     7381 ctcttgctac catgaccctt ttaactattt ttgcagatat atgaaagcgt tatccggact
     7441 tgacgttgtg accctcggct tgcctgccgg cctgcgggga aaaagaacag ggctgctgtg
     7501 tcattcgtca agcatttcat cagacttcag gcatattaca gagatattcg gacaaagcaa
     7561 agagtgcagg ctggtctctc tcttcgggcc gcaacacggg ctctcagggc aaacccagga
     7621 taatatgata gagtgggaag ggaccactga ccctctgctt ggcattcccg tctacagcct
     7681 ctacggcgag caccgcaaac caactgatgc aatgcttgag ggcattgagg tttttgttat
     7741 tgatcttcag gatgttggtg caagactata tacctatatc tggactgtta agctatgcat
     7801 ggaggcatgc gccgaagccg gcatacctgt ttacatactt gacaggccca acccgatagg
     7861 aaggatacaa cctgacggac cggttctgag cgaagagtac tttacattcg ttggcggtgc
     7921 gtctattcct ctctgccacc gcatgacaat gtgcgagatg gccatctggg tgaaggaaaa
     7981 acatatccct ggttgtgatc tgcatgtcat cagggcggaa ggatggagaa gaaacatgat
     8041 gtatcacgag actggcctgc catgggtcct cccctctccg aacatgccca ccccccagac
     8101 ggcagcagtt tacccgggaa cagttctcgc cgaagccctt aatatctccg aggcaagggg
     8161 cacggtgata ccatttgagc tcatgggtgc tccgtggatt gacggaagga gactgcttga
     8221 ggaaatgcgc agcagaaaac tccccggatg tcaattcagg atgcacgact tcatacccac
     8281 tttccataaa tacgcgggtg aatactgcag gggaatacag atacatgtca ctgatcccgc
     8341 cctcttcagg cctgtggcaa ctgccatgca cctgtttgat gctatcatcc ggacatcgcc
     8401 ggaaggctcc ctgaaattca accctccacc ctatgagtac gaataccgcc tgatgccttt
     8461 cgacattctt tcgggcgaca gccgtatgcg ggaggtgctg accggcggcc tctcgctgaa
     8521 ggatgaaact gagcgctggg aagcacagac ggaaacattc atggctgact tcaggcagat
     8581 tgccctatat ccggattaat atgatcactg ccggaaatat catcgccctc tcaataataa
     8641 tattcttagc ttcagtcgta agggggttca ccggcttcgg acttgcattg gtggcagtgc
     8701 cgctgataca gttcattttt cccgtatcag atactgccgt cttcattgcc gtcataaaca
     8761 tgatcttctc catactctac taccgccggt caagagagct ggtcagggaa cagccccttg
     8821 gaagaatggc actgttcacg ggagtgggtg ttgcagccgg agtactaatc cttaaataca
     8881 taaatccggc atatattcaa ctcacctggg ggatactgat aatattaatc gtaactgccc
     8941 ttgtcagggg agctaatctc aggattagtt cagatgcccc ggcaatgacg ttgagtggcc
     9001 ttttcggcgg cctccttgcc ggggctaccg gcataaccgg tccgccggtg gcaatcatcc
     9061 tctctgcaat gaagacgcca aaggaaaaat tcaacgcagt gatctcactc tttattctct
     9121 ttgcagtttc ttatgccctc ctcttctacc tgatatcggg tctcatccgc cgtgatactg
     9181 tattgcttgc attatgctcg gtgcctgccc tcattgcagg tctccgtact ggtgatattc
     9241 tcgtctcaag aataagtcag aagacattta cgaatgtgat atatatagtg cttataatca
     9301 tgggaataat cac
//
LOCUS       DAOPBK010000102         9219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7085_length_9219_cov_4.001309, whole genome shotgun sequence.
ACCESSION   DAOPBK010000102 DAOPBK010000000
VERSION     DAOPBK010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_9219_cov_4.001309"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLA17_07125"
     CDS             <1..416
                     /locus_tag="PLA17_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HPK85224.1"
                     /translation="GYKGKVILSGGTTADIVARELNRKIVDVLIFEDPDLPPESFLEG
                     IDLVTEGILTLQKVTELVKSYKNSVSLGKGPADKIVKMIMESDEINFLVGTRINVAHQ
                     DPTLPVDLEIRRTVVRRIARILEDKWLKQVSIDYI"
     gene            complement(435..845)
                     /locus_tag="PLA17_07130"
     CDS             complement(435..845)
                     /locus_tag="PLA17_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPK85225.1"
                     /translation="MELIPEGIRPLNISYVVIAARYKGGWLFVKHRRRGGWEMPAGHP
                     GPGENTVEAAVRELMEETGAVSFIMEPVAFYSVDTGTGKQYGRLFFADVELTGPHSDT
                     DEVEEVRIYRRLPRKLSLREVMSFLYRVAKRHAM"
     gene            931..2109
                     /locus_tag="PLA17_07135"
     CDS             931..2109
                     /locus_tag="PLA17_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPK85226.1"
                     /translation="MLFYLALLVTDLILWYILIRALKGRNMVLRIAVLLLKTAGTALF
                     IALFLKIVLYRGEFAEPSNAFRQVQMGVMALLLMLTTGACLAVSVTGRVAGALMKREM
                     KWPAVTNIVLFLLATLFVADGYFRQRLDIRIVRQDITVSGLDRALDGLTIALISDIHI
                     SSWQGDYDRLEAIMKQVGSEKPDLVLNTGDFISYGWQEFGMSDTILRKAESLAGAFAV
                     EGNHDDGTYYPAYDSRYGELCREMVKRSIEKSGYTLLRDSAVIISHRGVQMAVAGVET
                     HGHHLDMRYGDFEKALSQVPDSLFTILLLHDPESWHLAMASGRTPQLTVAGHTHGFQA
                     ALPAGIWSPASLFHKRWKGLYEYRGSHLYVTSGLGTMGMAARFFMPPEIVIITVKSGH
                     "
     gene            2179..3747
                     /locus_tag="PLA17_07140"
     CDS             2179..3747
                     /locus_tag="PLA17_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPK85227.1"
                     /translation="MRLKSLIVAAALLAALPSYPQQEALRAITLENSEAYMKFLSSDD
                     LEGRRTGSEGNNLAAEYIKAEALKLGVRPLPGQDDLFQELRYIKTSIVPGESRITFSD
                     SCSGHIFSADIMPLMPPSDTVSLEGEVVFGGYGYVNSEEEYNDLAGISLKDKIVVIMT
                     RIPEIFGSGMPAPGSGISEMTEVRKLSMIMLLQARAIIFVADPALGNDISSDLLSMGS
                     PYQLTPLFRKQIFSFSLNVYAITTGTADQMLGGSGLTLRKLQDSIASSLKPVSFIIPN
                     LRTNISVNVVRDTVTSNNIIGYVEGSDPVLKNEAVIFTAHYDHVGKDASGNIYNGAND
                     NASGSVGLLNIASAFSSLRKKPARSAIFLWTTGEEEGLHGSTHYADNPPFPLEKTVAA
                     INFDMIARSRQESDVGESLSGRIDITGPDTIRIIHGANCPELVTIATTACHESGIFPI
                     DDGKGANFSGSDHYPFYRKKIPVVFFFTGLHKDYHKETDDYEFIDFDKLVRVSKAGFA
                     TGYRVAMQPDFNVK"
     gene            3778..4476
                     /locus_tag="PLA17_07145"
     CDS             3778..4476
                     /locus_tag="PLA17_07145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPK85228.1"
                     /translation="MSKRRLALSSGEVEVSIVRSSRRTVALYVQPGGTLLIRAPWHVP
                     VRLLMQFAAQKSSWIERQINRLKHVKPPETPLAIHDGAAVPFMGRDLTVAIRRGSARR
                     ASRNDDMLVLTVTGDPLPEELTGMTESWYLREAKAYLPERTRWLAGQYSHLLPAPGSV
                     RVRKMKRRWGTCHKGGAIWLNRELMKKSPDLIDYVIIHELCHLVHHNHGKEYYELLGR
                     ILPGFRELRKRLQQ"
     gene            complement(4673..5905)
                     /locus_tag="PLA17_07150"
     CDS             complement(4673..5905)
                     /locus_tag="PLA17_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPK85229.1"
                     /translation="MALNYIWIAFFLIGFLFALGQLIFNGDTSVFNKIVESTFSSAKT
                     GFEISLGLTGVLTLWMGLMKVGEKGGAVSLLSRAIGPLFRRLFPKLPANSPAHGSMML
                     NVAANMLGLDNAATPMGLKAMKEMQEHNPDKEVASDPMIMFLVLNASGLVIIPVTIMV
                     YRAQMGAANPADIFIPILIATYIATLAGLISVAIAQKINLLNRVVLGYLGGLTALIGG
                     IVWYFSTLPQDQINTISTFAANFILLSVIVSFIVMAMVKKVNVYEAFIEGAKDGFKTA
                     VTIIPYLVAILVAIGVFRASGAMDLLVDGITAVVAWMGLDTSFTEALPTAFMKPLSGS
                     GSRGMMIDAMTTHGADSFVGRLTCCLQGSTDTVFYVLAVYFGSVGIKNTRYAVVCGLI
                     ADIVGSISAIFVAYLFFA"
     gene            complement(5969..6352)
                     /locus_tag="PLA17_07155"
     CDS             complement(5969..6352)
                     /locus_tag="PLA17_07155"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPK85230.1"
                     /translation="MEKLKIKETTKTPSVSFDPEHGLFEIRGKSIPENSSGFYGPLIQ
                     YVEKYAGNPAARTVLSVRFEFFNTSSSNRIHALFKRFERIFLADRDVLIRWYCENSDD
                     NMRDAGRDFKAILQVPFEIVEVDEL"
     gene            6634..>8778
                     /locus_tag="PLA17_07160"
     CDS             6634..>8778
                     /locus_tag="PLA17_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPK85231.1"
                     /translation="MNARVSFFMNTREFRVSTGTDGRYSVKITGSYSDVAGEFQAGTP
                     YPNPFDNSVNIPFIINTPGDVMLTIYSLAGEKVRIMVFPDVQEGSYNAVWDGCGQNNA
                     PMSAGLYIYAITFRGRSYSGRLIKATGTTSGSSGSGLEPVMLPPDTPMPGSALRFPVI
                     AEVTAPDYYPVRLTDITLVRDTVINFILTRKNAMPFTVAGNYIARYLDNDYKPMILKG
                     VNLGSSPPGYFPGEIAYAITAATYERWINRMGEAGFNSLRVYTLHPPVFYEKLAEYNQ
                     RNPDRPLLLFQGIWLEEVEDGSNPLCYDLIRRRSAFSAEIAEVIDCIHGNADIAFRYG
                     KAYGIYRTDISQWTAAYIIGREVAPQEIDSTNKFHPGNAFYSGTRFSISGGTASEAFI
                     AEMLDRVVVFEENKYSASRPVSFSSWPTLDPLEHPTEIFTDEDVASFDITKIVRSGGP
                     GLFASYHAYPYYPNFVSQDPAYMGFSDAQGPNSYLGYLTAMKSHYSNMPLVIAEFGVP
                     SSWSSAHQSYSSMHHGGYSEKQQGQKNMRMMHNMLDAGCAGGFMFAWMDEWFKPTWIV
                     LYQEAYGLNTGGEIIPTRQLWHNEMSPEQCFGLLAYEQKERPAFVPFTIDQTSGPVSS
                     VRATHDEGFLYVEINTAAELLPTDEFMVAFDTYLGNTGESTLPNGAITGNRAEFLLTF
                     ALEDDTALHHVTEAYDMNGLTVRFNLTDPAQKF"
     assembly_gap    8779..8788
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8789..>9219
                     /locus_tag="PLA17_07165"
     CDS             <8789..>9219
                     /locus_tag="PLA17_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85232.1"
                     /translation="KSTTTDGAPWKVMRWINDGFELTYHDIGKVPLEHASSFTEGDRA
                     AVAWNGRKITIRLPWTMLHFYDPTQMTVNDGAISYDGGYNFEIITAQSDGIAISVWHN
                     GVVTNTTNRYNWPKWLIVPQTVEREKASLHIIEAGLTAIPD"
BASE COUNT         2332 a         2340 c         2419 g         2118 t
ORIGIN      
        1 tggggtacaa gggcaaggtg atattatccg gtggcacaac cgctgatata gttgcacggg
       61 aactgaaccg caagatcgtc gatgtgctta tcttcgaaga ccctgacctg ccgccggaga
      121 gcttcctgga ggggatagac cttgttaccg aggggatact aaccctgcag aaggtgacag
      181 agcttgtcaa gagttacaaa aacagtgtca gtctgggaaa ggggccggct gacaagatcg
      241 taaagatgat aatggagagc gacgagatca atttcctggt cggcacacga atcaatgtag
      301 cccatcagga tcccacactt cctgttgacc ttgagatacg tcgtacggtt gtcaggcgca
      361 tagcccgcat tctggaggac aagtggctga agcaggtaag catagattat atctgagcag
      421 ccaggtgtta ctacttacat cgcatgccgc ttcgcaaccc tgtagaggaa tgacatcact
      481 tcacgaaggg agagttttcg tggcaggcgc ctgtatatcc gtacctcttc tacctcgtca
      541 gtgtcagagt gtgggcctgt cagctcaaca tcagcgaaaa agagcctgcc atattgtttt
      601 ccggttcctg tgtcaaccga ataaaaggcc accggctcca tgatgaagct gaccgctccc
      661 gtctcctcca tcagctcacg cactgccgcc tcaaccgtat tctctcccgg ccccggatga
      721 ccggcaggca tctcccagcc tcccctcctg cggtgcttca caaacagcca tccgccctta
      781 taacgggcag caattacaac gtatgatata ttcagcggcc tgatgccttc aggtattaat
      841 tccacagtca ccatgcggta aaattaaagc aaaaagagaa ttatgtgccg gagtatctct
      901 taaacattac ttttgtcaaa aacttggcag atgttattct acctggcatt actggttact
      961 gacctgattc tctggtatat cctgatcagg gcactgaagg gaagaaacat ggtgctccgt
     1021 attgctgttt tgttgctcaa gacagcaggg acagccctct ttattgcact cttcctgaag
     1081 atagtactgt acaggggtga gtttgccgaa ccgtcaaatg ccttcaggca ggttcagatg
     1141 ggggttatgg cactgctgct gatgttgaca accggagcat gcctggcagt gtcagttaca
     1201 ggccgtgttg ccggggccct gatgaagcgg gagatgaaat ggcctgcagt gaccaatatt
     1261 gtcctgttcc tccttgcaac gctttttgtt gccgacggct acttccggca gagacttgat
     1321 atccggattg tgaggcagga tatcacagtc agtggacttg accgggccct tgacggatta
     1381 acgatagctc tcataagcga tattcatatc tcttcatggc agggggacta tgaccggctt
     1441 gaggcgatca tgaagcaggt cggcagcgag aagccggacc ttgtgctgaa cacaggcgat
     1501 ttcatctctt acggctggca ggagttcggt atgtctgaca ccatactccg taaggcagaa
     1561 agtctggcag gagccttcgc cgttgaaggc aaccatgacg acggaacata ttatcccgcc
     1621 tatgacagca ggtacgggga gttgtgccgt gagatggtca aaaggagcat agaaaagtca
     1681 ggttacaccc ttctgagaga cagtgcagtc atcatcagcc acaggggtgt gcagatggca
     1741 gttgccggag tggaaactca tggtcaccat cttgacatga ggtacggtga ttttgagaag
     1801 gcactgagcc aggtacccga ttcgctgttc acgatcctgc ttctgcacga tccggaatca
     1861 tggcatctgg ccatggcttc aggaagaact ccccagctca ctgtggcagg gcacactcac
     1921 gggtttcagg cagcccttcc cgcgggtatc tggtcaccgg cctcgctgtt ccacaagaga
     1981 tggaaagggc tgtatgaata caggggcagt catctatatg ttacctccgg ccttggcaca
     2041 atgggtatgg cagcaagatt tttcatgcca cctgagatag tgattattac tgtgaaatca
     2101 ggtcattgat ttatcaggat ataaacaaca acctatcccg catttgtgta aatttgcagt
     2161 tcataaactg tcaacagcat gagacttaaa tctctgatcg tagctgccgc cctgctggca
     2221 gcactgccat cctacccgca gcaggaagct ctgagagcca tcaccctgga gaactcggaa
     2281 gcttacatga aattcctctc atctgatgat cttgagggga gaaggacagg gagcgaaggg
     2341 aataacctgg ctgcagagta cattaaggct gaagccctga agcttggtgt acgtcctctt
     2401 cccggccagg atgatctgtt tcaggaactc aggtacataa agaccagcat agtccccggc
     2461 gaatcacgta taacattcag tgacagctgt tcgggccata tcttctctgc cgatatcatg
     2521 cccctgatgc ccccttctga cactgtaagc cttgagggcg aagtggtttt tggagggtac
     2581 ggatacgtta acagtgagga agagtacaat gacctggcag ggataagtct caaggacaag
     2641 attgtcgtaa ttatgacaag gataccggag attttcggga gcggaatgcc ggcaccgggc
     2701 agcggcatca gtgagatgac cgaagtcagg aaactatcca tgataatgct tctgcaggcc
     2761 agggcaatta ttttcgtagc tgatccggca ctcggaaacg atatctcctc cgaccttctc
     2821 tcaatgggtt caccctacca gctgaccccg ctcttcagga aacagatatt tagtttttca
     2881 ttgaacgttt acgccatcac caccgggacg gctgaccaga tgcttggcgg atcagggctt
     2941 accctcagga agcttcagga cagcatagcc tcatcactca agccggtatc gtttatcatc
     3001 ccgaacctca ggacaaatat cagtgtcaat gtggtcaggg ataccgttac aagcaacaac
     3061 attatcggat atgtagaggg atctgatccg gtactgaaga atgaggctgt gatcttcacc
     3121 gctcattatg accatgttgg caaggatgct tccggcaata tctataacgg tgcaaatgac
     3181 aacgcctcag gcagtgttgg tctgctgaat attgcttccg ccttctcatc cctcaggaag
     3241 aaaccggcga gaagtgcaat cttcctgtgg accacaggcg aggaagaggg gctccatgga
     3301 tcaactcatt atgccgataa tcccccgttc cctctcgaaa aaaccgttgc agccatcaat
     3361 tttgacatga tagcgcggtc aaggcaggag agcgatgtgg gggaatcact ttccggcagg
     3421 atagacatca ccggccctga cacaatcagg ataattcacg gcgccaactg tccggaactg
     3481 gtcaccattg ccaccactgc atgtcacgaa tcaggcatat tcccgataga tgatggaaaa
     3541 ggagcgaact tctcgggatc agatcattat cccttctacc gcaagaagat tcctgttgtt
     3601 ttctttttca ccggccttca caaggattat cacaaggaga cagacgacta tgagttcatt
     3661 gattttgaca agctcgtccg tgtgtcaaag gcggggttcg ccaccggtta cagggtggca
     3721 atgcaacctg acttcaacgt taaatagatg atcctgaggg gttactgaat ccgcagcatg
     3781 agcaaaagga gactcgccct ttcgtcgggg gaggtagaag tcagtatagt gaggtcgtca
     3841 aggcggacgg tggcgcttta tgtgcagccg ggaggcaccc tgctcatcag ggctccgtgg
     3901 catgtgccgg tccggctgct catgcagttt gcagctcaaa aatcgtcatg gatcgagcgc
     3961 cagataaaca ggctgaagca tgtaaagcct ccggaaacac ctcttgccat ccatgacgga
     4021 gctgccgtgc catttatggg gcgcgacctg actgtggcca tcaggcgtgg atcagcccgc
     4081 agggcttccc gtaatgatga tatgcttgtt ctgacggtga ccggtgatcc gttgccggag
     4141 gagctgacag ggatgacaga gtcatggtac ctgcgtgagg cgaaggcata cctgccggag
     4201 cggacgaggt ggcttgcagg gcaatactcc catctgctgc cagcaccggg gagtgtcagg
     4261 gtaaggaaga tgaaacgccg ctgggggacc tgtcacaagg gcggagcgat ctggctgaac
     4321 agggagctga tgaaaaaatc acccgatctg attgactatg tcattattca tgaactgtgc
     4381 cacctggttc atcacaacca cggaaaggag tattatgaat tactgggccg gattctgccc
     4441 ggtttccggg agctgagaaa gaggctgcag cagtgagccg ggccggcggt gagcagcccg
     4501 gcaagctggc aaaaccagcc agtcagcagt accaggcaga cgacagtgag aagtgagccg
     4561 ggccggcggt gagcagcccg gcaagctgtg agcggtgggt cagtcggccg tgaacagtca
     4621 ggcaagttgc agcagtgagt cgggccggcg gcgccagttg gccggaagtg actcaggcaa
     4681 agaagaggta tgccacgaaa atggccgaga tgctgcccac tatatcagct atcagtccgc
     4741 ataccactgc atatcttgta ttcttgattc ccaccgagcc gaagtagact gccagcacat
     4801 agaaaacggt gtcagttgat ccctggagac aacatgtcag ccgtccgacg aatgagtcgg
     4861 cgccatgtgt tgtcatggca tctatcatca tcccccgcga gccgcttccg ctcagcggct
     4921 tcatgaaggc cgtcggcagc gcctcagtaa aggaggtatc aagccccatc caggctacca
     4981 ctgccgttat gccgtcaaca agcagatcca tggctcccga tgccctgaag acacctattg
     5041 ccacaagtat ggcaacaaga tagggtatga ttgtgacggc tgtcttgaat ccatctttgg
     5101 ctccctcgat gaaggcctca taaacattga ccttttttac catcgccatc acgatgaatg
     5161 agactataac gctgagaagg atgaagttcg ccgcaaaggt cgagattgta ttgatctgat
     5221 cctgcggcag ggtgctaaaa taccagacaa tgccgccaat gagtgcggtc agacctccca
     5281 ggtaaccaag caccaccctg tttaaaagat taatcttctg ggcaatggcc acacttatca
     5341 gtccggcaag ggtggcaata taggttgcta tcagtatggg aatgaatatg tcagccggat
     5401 tggctgcccc catctgtgcc ctgtaaacca tgatggttac gggaattatg acaagccctg
     5461 atgcattcag caccagaaac attatcatgg ggtctgaagc cacctccttg tcagggttgt
     5521 gctcctgcat ctccttcata gccttcagcc ccatgggggt ggcggcattg tcaaggccaa
     5581 gcatgttggc agccacgttg agcatcatgg agccgtgtgc aggactgtta gccggaagtt
     5641 tcgggaaaag tctcctgaac agcggcccga tagccctgga aagcaggctg acggcaccgc
     5701 ccttctctcc cactttcatc agccccatcc acagggtaag tacaccggtc agtccgaggg
     5761 atatctcaaa accggtcttt gcacttgaga aggttgattc tacaattttg ttgaaaaccg
     5821 aggtgtcacc attgaagatg agctgaccca gggcaaacag aaacccaatc agaaaaaaag
     5881 caatccagat atagttcagt gccatccgct aaaattctca gtgtctaaaa cagggaaaaa
     5941 tcactttcgc aaaagattgt tgataaaatc aaagctcatc cacttcgaca atctcaaacg
     6001 gtacctggag aattgccttg aaatctctcc cggcatccct catattgtca tcgctgtttt
     6061 cacagtacca tcttatcagt acgtccctgt cagccaggaa aatcctttca aatcgcttga
     6121 agagcgcgtg tatcctgttt gaagagctgg tattgaagaa ttcaaacctg acactaagca
     6181 ctgttcttgc agcaggattg ccggcatatt tttcaacgta ctgaatgagg ggcccgtaaa
     6241 aaccgcttga gttctcagga atggactttc cccgtatctc aaacagacca tgctccgggt
     6301 caaagcttac cgagggggtt ttggtggttt ccttgatctt tagtttttcc ataaatatga
     6361 agccacagca aagtataaaa gtatggattt cctccaatat agacaaatca tgggccaatg
     6421 ttgaaaaatt tccgggttat taacaacgct ttgtgttaca tttggtatgg atagttgttc
     6481 attatcagtt gttaccggga aaagacaagt atggccatgg atattatacg tttgtttatc
     6541 agcatcttat gtctgacggt atctattcca caagtttttt ctgaggatat tacagtaacg
     6601 ggcaccgttt caggccgcga cgggtcaccg gtgatgaatg cgcgtgtatc cttctttatg
     6661 aataccaggg agttcagggt ttcaacagga actgatggcc gctattcggt gaagataacc
     6721 ggttcatact ctgacgttgc aggtgagttc caggcaggca ccccctaccc gaacccgttt
     6781 gacaacagtg tcaacatacc ttttattatc aacaccccgg gagatgtaat gctgacaata
     6841 tacagcctgg ccggggaaaa ggtaaggata atggtctttc ctgatgttca ggaaggcagt
     6901 tacaatgcag tatgggacgg atgcgggcag aacaatgccc cgatgagtgc cgggctatat
     6961 atctacgcca taaccttcag gggccggtca tacagcggaa gactcatcaa ggctaccggc
     7021 acaacttccg gctcatcggg aagcgggctg gaacctgtga tgctgcctcc tgatactccc
     7081 atgcccggat cagcactccg gttcccggtt atcgctgaag taacagcccc ggactactac
     7141 ccggtaagac taactgacat aacccttgta cgcgatactg tcatcaactt cattctgacg
     7201 cgtaaaaacg caatgccctt tacggtggcg ggaaactata tcgccaggta cctggataac
     7261 gattacaagc caatgatcct aaagggggta aaccttggct cttcacctcc cggttacttc
     7321 cccggtgaga ttgcatacgc catcactgcc gccacctacg agagatggat caaccggatg
     7381 ggggaggcgg gattcaactc gttaagggtt tacacccttc atccgccggt attctatgag
     7441 aaacttgcag aatacaacca gcgtaatccg gacaggccgc tgctgctctt ccagggaata
     7501 tggctcgagg aggttgagga tggctccaac ccgctgtgct atgaccttat caggcgccgg
     7561 tcagcattca gcgcggagat tgctgaggtg atcgactgca tccatggcaa tgccgatatt
     7621 gcattcaggt atggcaaggc atacggcatt tacaggactg atatttcgca atggactgca
     7681 gcgtatatca taggccgcga ggttgctccg caggagattg actcgacgaa taaattccat
     7741 cccggcaatg ccttctattc agggacacgt ttcagcatca gtggcggcac ggcatctgaa
     7801 gcttttatcg ctgagatgct tgacagggtt gtggtcttcg aggagaataa atactcggcc
     7861 agcaggcctg tcagtttctc gagctggccc acgcttgacc cgctggaaca ccccacggag
     7921 atctttaccg atgaggatgt ggcttcattt gacattacaa aaattgtccg cagcggaggg
     7981 cccggcctct ttgccagcta ccatgcctat ccctactatc cgaatttcgt cagccaggac
     8041 cctgcataca tgggtttcag tgacgcacag ggtccgaaca gctacctggg ttacctcact
     8101 gccatgaaga gtcactacag caacatgccg ctggtgatag cagagtttgg cgttccctca
     8161 agctggagca gcgcacacca gtcgtacagc tcaatgcatc acggcggtta ctcggagaaa
     8221 cagcaggggc agaagaacat gcggatgatg cataacatgc tcgatgccgg ctgcgccgga
     8281 gggttcatgt ttgcatggat ggatgagtgg ttcaagccta catggatagt tctttaccag
     8341 gaggcttatg gcctcaacac cggaggagag atcataccca cacgccagtt gtggcataac
     8401 gagatgtcgc cggagcagtg cttcggcctc ctggcctacg aacaaaaaga gagacccgct
     8461 tttgtgccat tcactattga tcagacatca gggccggtaa gctctgtcag ggcaacacat
     8521 gacgaaggat tcctctacgt tgagatcaac acggcagccg aactgttacc aaccgatgaa
     8581 ttcatggtag cttttgacac ctacctcgga aataccggtg aatcgacact tcccaacggc
     8641 gccatcacgg ggaacagggc cgaattcctg ctcactttcg cgcttgaaga tgacacggcg
     8701 ctgcatcatg tgacggaggc ctatgacatg aacggcctga cggtacggtt caatctcact
     8761 gacccggcgc agaagtttnn nnnnnnnnta aaagcaccac cacagacggc gctccgtgga
     8821 aggtaatgcg gtggatcaat gacggcttcg agctcacata ccatgatatt ggcaaggtac
     8881 cgcttgaaca cgcctcctcc tttactgaag gagacagggc agcagtggcc tggaatggcc
     8941 ggaagataac catcaggctg ccatggacca tgcttcattt ctatgacccc acgcagatga
     9001 cggtcaatga tggcgcaatc tcatatgatg gcggttacaa ttttgagatc atcaccgcgc
     9061 agtctgacgg aatagcaata tcagtctggc acaatggtgt tgtcaccaat accacaaaca
     9121 ggtacaactg gccaaagtgg ctgatagtac cgcagaccgt ggagagggaa aaggcatcgc
     9181 ttcatatcat agaggcaggg ctcaccgcga ttccggatt
//
LOCUS       DAOPBK010000103         9092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7199_length_9092_cov_4.558814, whole genome shotgun sequence.
ACCESSION   DAOPBK010000103 DAOPBK010000000
VERSION     DAOPBK010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_9092_cov_4.558814"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..3654
                     /locus_tag="PLA17_07170"
     CDS             322..3654
                     /locus_tag="PLA17_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPK85233.1"
                     /translation="MNRLKLMILLFVLTGFTALMAQTVTITGTVTSADDGMPIPGVAV
                     TVQGTTLGAFTDADGKYLINAPTSAANLTFTFVGMKTITERISGRTTIDVVMQTEMHG
                     LDEVIVTALGISRERKALGYAVQDVKSEELTRAAQANVISSLSGKIAGLQVSQAGGQI
                     GASSRIVIRGNSSLGDNQPLIVIDGIPVSNSASRQNSVDFGSGLYDINPDDIESISVL
                     KGGSAALYGMRAGHGVILITTKSGSRQKEGLSVTYDGNFNVDQVYSLLKMQNKYGQGY
                     LGDEWWYKLACAGQLDRNDDKVLDELDIFEGSYAEFAQGDFNPGYGFSYLDGLGNGVN
                     DGVDESWGPRLDDGLKLTQYNSPRDSNGNLIPTDWVSSPNNMKDLYQFGYTTNHNIAV
                     TSNSERSTTRASVGYRNQKGTLPNTDLKRYTINFNTNVKLSKMFDFDLSSNYTRSESS
                     NLPMTEYNASNPMQSMGQWFGRQVDMKDLKEHWTETMVNGYPYNWNSNYHNNPYWSLN
                     KNTNSMDKDRLFGKTSLYFKPVSFITIEGRLGVDYYNQKTMSVISYRSNEMMAGAGSW
                     DGGSFNLYDTKNYEFNADVIATFHKKINVFDVDLLAGANYRNLKWEYSRIGANLLTVP
                     DLFTIANVKGSPLNAMDHSWIRSNSVYGSASVGYNGMIYLEGSARNDWSSTIADPFFY
                     PAVSVSWIPTETFKINSTFLNYLKIRGGWAKIGSATGAYRTDPYFSAGTYTIYGVTQY
                     SQTTEFPPTGLRPEQVVTRELGLETRLLNNHLTIDMSLYDKVTTDQILPVAISKATGY
                     NTMLINAGEISNKGIEIQLRGNIFNKSDGFNWDITVNWSKDQSKIIDLYTDPVTNQPL
                     ASYNIGSQWSTYVQARPGEPWGVIVGNGMVRRESDNAIIVNSAGMPRLRSSMIIGNVN
                     PDWLAGIRNDFSYKGLSFGFMIDLRKGGDIFSISHMFGTYSGLTEFTAAGEIRSNGLV
                     LGKDFLKDEKFVKIVNQNPDDIQKSEFAENDIVIGAQDFFESYYSNRELSVIDGSYVK
                     LREAYLSYTLPPSLFSATNMIKSGSISLVGSNLAILLRHKSNISGLDPESTVTSDNNG
                     VGLETTSFPPTRSMGIRLSFTF"
     gene            3675..5138
                     /locus_tag="PLA17_07175"
     CDS             3675..5138
                     /locus_tag="PLA17_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPK85234.1"
                     /translation="MKRYILKLGILAVFSAFILTSCTESFPEVNTDPDRAKDAPATNV
                     LAFTLRYYGSTFFDPWADMNELSTYAGYLTKIQYIDEARYNFRPGVVENHWYYGYILL
                     NNINEIKKKAIADGADNLLGVAKVMEVLIVQNMTDRWRDIPYSDAIKMAEGVLLPTYD
                     TQEQIYPALLAVLAEANTLLSTPAAADQLGDGDLLYNGSVKAWQKLANSLRLRLAMRI
                     SGVAPDVAKQVVETILGNPAANPVMTANKDNAMIVWPGTSPYEEPWYADSKGRDDHGV
                     SDVLVDALKDLEDPRLPVYALPATADGEYRGFIIGALAQPSLPTISRIGTRFRKDPAG
                     FSPVFRYAEVEFCIAEAAKKGWATGTTTQEAYEAGVRASLEENGIDNADIEAYLTTGK
                     GKFEDTFEQIYWQQWIALYKQGMEGWSLYRRTGVPTTHYVAPGSPYPGHNAPPFRYPY
                     PDNEGTLNGANSKTFRDQVVDNFWGKQMWFDQRTGLQ"
     gene            5642..6211
                     /locus_tag="PLA17_07180"
     CDS             5642..6211
                     /locus_tag="PLA17_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85235.1"
                     /translation="MIGSPYLSDDWCEGSIALYNGDIIDNIALKYNVFKKEMLFQAGG
                     DIYVLDSPDSVLFITMGDRRFVYLPVNDRKKKSEKDYFEELSDGDACRLLLRHTASIL
                     KSNYNVALNVGEKDDRMEHQSAYYLRKDDLVVPVDRKGENLYRLLADKSAELRDFVKE
                     RNLSFASQDDLKQIIDYYNSFYLLPDGVD"
     gene            complement(6359..7780)
                     /locus_tag="PLA17_07185"
     CDS             complement(6359..7780)
                     /locus_tag="PLA17_07185"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPK85236.1"
                     /translation="MKKLTRTIRLSYILADFTGSSVAWGLFYLFRKYFIETKLYGDYF
                     QMELGTRFYLGISLIPLFFLFVFAMAGFYSDPLRRSRLLEFGKSLAITLAAVVVLFFL
                     LILDDVIGHYSTYYSSFGVLFLLLFMLTYIPRLIITSVVSGKIHRREIGFRTLIVGSR
                     EKAARLISEIRSEKMPAGNLLIGFLTVSGDSKQQSDVDIRRLGSADEIIRVIEEHEIN
                     EVILAMEEDEYGQVEEIVNAVCYRDVVVRAVPSMRVVISGQVEAGPIYATPLLRVSYR
                     EMPRWQYMIKLLMDYSLSLLALVVLSPLMAVLALLIRLSGKGPVIFRQERIGRYGKPF
                     MIYKFRSMEEDAESDGPKLAVKGDSRITTVGRFMRKHRFDEIPNFINVLKGEMSLVGP
                     RPERSHYIEQIRVRAPWFNRILAVKPGITCWGQVKLGYASDLDTMLERLSYDLLYLEN
                     ISLFLDLKIIFYTLGAIIKGKGL"
     gene            7837..8502
                     /locus_tag="PLA17_07190"
     CDS             7837..8502
                     /locus_tag="PLA17_07190"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPK85237.1"
                     /translation="MMIEESFEAKGKRKKLIEELRRKGINDERVLEAMGKVPRHLFMD
                     DAFLRHAYQDKAFPIAAGQTISQPYTVAVQTELLRVGKRDKVLEVGTGSGYQAAVLAE
                     MGVRVYTIERQRELYRKAQIMLGELGYRVHFFLGDGYEGQPQYGPYDGIIITAATDEV
                     PGELLKQLRTGGRLVVPKGKRDLQVMTLYERRGEDDYEITTHGNFIFVPMLRGIANTN
                     NRE"
     gene            8507..>9092
                     /locus_tag="PLA17_07195"
     CDS             8507..>9092
                     /locus_tag="PLA17_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK85238.1"
                     /translation="MKIYRAIFSPIQVNTYIITGNGSECVIIDCGCYGGAEEQKLEEM
                     LSARELKPVMLLNTHCHLDHVFGNRFMLDRYGLRPWFHEGDRHNYRNAPKHSLIWGLS
                     MEPQPDPEGYLSDGQVISAAGVTLTVIAVPGHSPGGVAFYSEADAVVFTGDALFAGSI
                     GRSDLPGGDHEQLIENIRERLFVLPPDTVVYPGHG"
BASE COUNT         2537 a         2004 c         2246 g         2305 t
ORIGIN      
        1 agtactatag ctgctttcat atatatatta tggtatgttt tgttaatcgt aataaagata
       61 gcaaaatccc tgattttttt catgtttcag cagcttataa attcattgac tccggctttt
      121 gccctgaaaa acttcagtaa attcagtgaa ttgccgtttt tttttgtatg accggttgaa
      181 aagagtagaa aatcatttgc ataaaataaa aaagtctcta cttttaggga cttcttgtaa
      241 ggcatattct gccctgattg caggctgtgt caagcaggag gaggtgaaaa atacctgatt
      301 gtctaactta aactctgatc tatgaacaga ctaaaattga tgattttgct gtttgtgttg
      361 acgggcttca cagccctgat ggctcagacg gtgacgatta ctggtactgt cacgtcggca
      421 gatgacggaa tgcctattcc gggtgttgct gtgacggtgc agggaacgac acttggagca
      481 tttacggatg cagacgggaa gtacctgatc aatgcaccaa ccagtgctgc aaatcttacc
      541 ttcacttttg tggggatgaa aacaatcact gaaagaatca gtggccgtac cactattgat
      601 gtggtaatgc agaccgagat gcacggtctt gacgaagtta ttgtcacagc tctcggtata
      661 tcacgtgaga ggaaagctct gggatatgcc gtgcaggatg ttaaaagcga agaattgacc
      721 cgggcagcac aggcgaacgt catttcttcc ctgagcggga aaattgccgg actacaggtc
      781 agccaggcag gtggtcagat cggagcatca tcccgtatcg ttatccgcgg gaacagctca
      841 ttgggcgaca accagccgct tattgtcatt gatggtattc cggtatcaaa cagcgcatca
      901 agacaaaaca gcgttgactt tggatcagga ttgtatgaca tcaaccctga tgatattgaa
      961 tccatctcag tccttaaagg tggttcagca gcgctctacg gaatgcgcgc cgggcatggg
     1021 gtcatactga tcacaaccaa atccggcagc aggcagaagg aagggctgtc agtaacttat
     1081 gacggcaact ttaacgttga ccaggtatat agcctgctga agatgcagaa taaatacggg
     1141 caaggatacc ttggagacga atggtggtat aaattggctt gtgcagggca gcttgacaga
     1201 aatgatgaca aggttcttga tgaattggat attttcgaag ggagctatgc ggagtttgcc
     1261 cagggtgatt ttaacccggg gtatggcttt agttatcttg atggtttggg taacggagtc
     1321 aatgatggtg ttgatgaaag ctggggcccc cgcctggatg acgggttgaa actcacccaa
     1381 tacaacagcc cacgcgattc aaatggcaac ctgattccaa cagattgggt atcaagccct
     1441 aacaacatga aggatctgta ccagtttggg tatacgacca atcacaatat tgccgttact
     1501 tcaaactcgg aaaggtcaac aacaagggct tccgtaggtt accgtaatca gaaaggaacg
     1561 ctgccaaaca ctgatctgaa acgttacaca ataaacttta atacaaatgt caagctgagt
     1621 aagatgtttg atttcgatct ctcatcaaac tacaccagaa gtgaaagcag caacctcccc
     1681 atgacagagt ataatgcaag taacccgatg caatctatgg ggcaatggtt cggacgtcag
     1741 gttgacatga aggatttgaa ggagcactgg actgagacaa tggtaaacgg ttatccttac
     1801 aactggaaca gtaactacca caataaccct tattggagct tgaataagaa caccaactca
     1861 atggataagg acaggctatt cgggaaaacc tccctctact tcaaacctgt ttcattcatc
     1921 acaattgaag ggcgcctggg ggtagattat tacaaccaga agacgatgtc ggttatatcc
     1981 tatcgttcga atgaaatgat ggccggagca ggctcatggg atggcggaag cttcaatttg
     2041 tacgatacaa agaattatga attcaacgct gatgtgatcg caacctttca taagaagatt
     2101 aacgtctttg atgttgatct tctcgccggt gccaactatc gtaacctgaa atgggagtac
     2161 tcacgtattg gcgcaaatct cctgactgtt cccgatcttt tcactattgc aaacgtcaag
     2221 ggaagtcctc tgaatgcaat ggatcattcg tggatccgca gtaacagcgt ctacggatcg
     2281 gcatcagtcg gatataatgg catgatctat cttgaaggaa gcgcccgtaa tgactggagc
     2341 tctaccattg cagatccttt cttctatccg gctgtcagtg tgagctggat ccccactgaa
     2401 acattcaaga ttaattccac attccttaac tatctgaaga taagaggtgg atgggccaag
     2461 atcgggtcag ctaccggcgc ctacagaaca gatccttatt tctctgccgg aacatatacg
     2521 atctatggag taacccagta cagccagaca acggaattcc ccccgacagg cttgaggcct
     2581 gaacaggttg ttaccaggga actgggcctt gaaacccggt tgctcaacaa ccatctcaca
     2641 atagatatgt cactttacga taaagtcact accgaccaga tcctgcctgt cgctatttca
     2701 aaagcaacgg gatacaatac catgctcata aatgcaggtg agatcagcaa caaaggtatt
     2761 gagatacagc ttcggggaaa tatatttaac aagagtgacg gattcaactg ggatattact
     2821 gttaactggt ctaaagacca aagcaagatc atagacctgt atactgatcc ggtgaccaat
     2881 caaccgctgg catcatacaa catcggaagc cagtggagta catacgttca ggcaagaccc
     2941 ggtgaaccat ggggagtaat tgtaggaaac ggtatggttc gcagagaatc agacaatgca
     3001 attatcgtta attcagccgg tatgccaaga ctgaggtcga gcatgattat tggaaatgtt
     3061 aaccccgact ggctggcagg tatcagaaat gatttcagct ataagggtct gagcttcggc
     3121 tttatgattg atcttcgcaa gggaggggat atattcagta tctcccacat gtttggaacc
     3181 tattccggtc tgactgaatt tacagccgct ggagaaatcc gtagtaatgg acttgtgttg
     3241 ggaaaagact tcctgaaaga tgagaaattc gtaaaaatag tcaaccagaa tcctgatgat
     3301 atccagaaat cggaatttgc tgaaaatgac atagttattg gcgcccagga tttctttgaa
     3361 agttactaca gtaatcgtga actcagtgtc attgacggtt cgtatgtaaa acttcgtgag
     3421 gcatatttat cctacacttt gcctccgtca ctcttctcag cgaccaacat gataaagtca
     3481 gggtcaatct ctctggtagg ttccaactta gccatcttat tgagacataa gtccaatatt
     3541 tccggtcttg atcctgaatc aacggttacc agcgacaata acggtgtagg ccttgaaaca
     3601 acaagctttc ctccgacacg cagtatgggt atcaggctca gctttacatt ctaaatcatt
     3661 taagacaaag aaatatgaag agatatattt taaaacttgg aattctggca gtcttttccg
     3721 cattcatact tacgagttgt acggaatcgt ttccggaagt caataccgac ccggacaggg
     3781 ctaaagatgc tcctgccacc aatgtgctgg cattcacttt gcgttattat ggcagcacat
     3841 tctttgatcc atgggctgac atgaatgagc tttcgactta tgccggctat cttacgaaga
     3901 tccagtatat agacgaagca cgttataatt tcagacccgg cgttgttgaa aaccactggt
     3961 attatggtta catattgctg aataatatca atgagattaa aaagaaagcc atagcagacg
     4021 gcgctgacaa tcttcttgga gtggccaagg ttatggaagt tctgattgtt cagaatatga
     4081 cagaccgctg gagggatatt ccatacagcg atgcaatcaa gatggctgaa ggagtgcttc
     4141 tgcctaccta tgatacccag gaacagattt atcctgccct gctcgctgtt ctcgctgaag
     4201 ccaacacttt gctgtcaacc ccggctgccg cagatcaact tggtgatgga gacttgctct
     4261 ataatggcag tgtaaaagca tggcagaaac tggccaattc tctcaggctg agattagcaa
     4321 tgcgtatttc cggagttgca cctgatgtgg caaaacaggt ggttgagaca atacttggaa
     4381 atcctgcagc taaccctgtg atgacagcca acaaggataa tgccatgatt gtgtggcccg
     4441 gcacttcacc atatgaagag ccctggtatg ctgattcaaa gggcagggat gaccatggtg
     4501 taagcgatgt gctggtagat gcactgaaag acctggaaga cccccgttta cctgtttatg
     4561 ctcttcctgc aacagctgac ggagagtaca ggggctttat cataggtgca ttggctcagc
     4621 catccctgcc gactatttca agaatcggaa cccgtttcag gaaggatccg gccggattct
     4681 cacctgtatt cagatatgcc gaggttgaat tctgtatagc agaagcagca aaaaaaggat
     4741 gggcaaccgg aacaaccaca caggaagctt atgaagcagg cgtaagggcc tcactggagg
     4801 aaaacggtat tgacaatgcc gatattgagg cttatctgac aacaggaaaa ggcaaatttg
     4861 aagatacctt cgaacagatt tactggcagc agtggatcgc cttatataag caagggatgg
     4921 aagggtggtc attgtaccgc agaacaggtg ttccaactac tcattacgtt gcaccgggtt
     4981 ctccttatcc cggtcataat gctcctccgt tcaggtatcc ctaccctgat aatgaaggta
     5041 ctcttaatgg tgcaaacagt aaaaccttca gagatcaggt agttgataat ttctggggca
     5101 agcagatgtg gtttgatcaa agaacaggat tacaatgatt ggtagtcttt aattgatgac
     5161 agtagttttt gagacctccc tcctttatcg tggggaggtc tctctttagg tacgcccgga
     5221 atggacgata actacagggt gggagccccg aacgcaccct gacagcagga agggtttgat
     5281 aaaggaaagg atgacccggg cgaccgggaa cctgaaggaa gcctgaggca aattcccggt
     5341 ctgacggaca ggaacttcat ttaaggcatg aggcagggat tggtaaatac taaaccttta
     5401 ttcaggctga tgcggaaaaa ggctacagac acaaggccgg gagctcctgt gcaaattcat
     5461 tccatggtat agattttgac gataaaatca ttgattaaca gttaagctca tgtctttacg
     5521 tttgaatttt tgtttggccg ttttactatt taactgcggg acattgctcc ctcagctata
     5581 ttatgtgaag atgcccgcag ggtatgataa aatacatacg accaacgaca gagtgtccgg
     5641 gttgattggg tctccttatc tttccgatga ttggtgtgaa ggaagtattg ccctgtacaa
     5701 tggtgacatc attgacaata ttgccttaaa gtataatgtc tttaaaaaag agatgctctt
     5761 ccaggctggt ggcgatatct acgttctgga ctcgcctgac agtgtgctct ttattacaat
     5821 gggtgacaga cgttttgtat acctcccggt taatgacagg aaaaagaagt cggagaaaga
     5881 ctatttcgaa gagttgtctg acggtgatgc gtgccgctta ttgttaaggc atactgcatc
     5941 aatacttaaa tcgaattata atgtagcgct gaatgtcggc gaaaaggatg atcgcatgga
     6001 acaccaaagc gcttattatt taaggaagga tgatctggtt gtgccggttg acaggaaggg
     6061 agagaacctg taccgtctgt tagcagataa atctgcagaa ctacgtgatt ttgtaaagga
     6121 aaggaatctc tcttttgcca gtcaggatga tctgaaacag atcattgact actataattc
     6181 tttttatctg ctcccggatg gcgtagactg atcagctggt ttgcctgtga tattcgtctt
     6241 caactgacga tttgtttttt gcggtaatgt ggtcaaaaat agttggtaat tttttaagga
     6301 ttgaggatta aatctggatt gtcaacctga agagaaagcg gcagcctgtc caggtgattc
     6361 acaaaccctt gccttttatt attgccccca gggtgtagaa gatgatcttc aggtcaagga
     6421 acagcgagat attctccagg tagagcaggt catacgacaa tctttcaagc attgtatcca
     6481 ggtcggaagc atatccgagc ttcacctgcc cccagcaggt aatgcccggt ttcacggcaa
     6541 gaatacggtt gaaccatggc gccctcaccc tgatctgctc tatataatgg ctccgctcgg
     6601 gcctcggccc tacaagtgac atctcacctt tcagcacatt gatgaaattg ggaatctcat
     6661 caaaacggtg ctttctcatg aacctcccaa cggtggtgat ccgggagtca ccttttaccg
     6721 ccagcttcgg accgtcagac tcggcatcct cctccattga cctgaacttg tagatcatga
     6781 agggcttgcc gtacctgcca atgcgctcct gcctgaagat gaccgggccc ttcccggaaa
     6841 gcctgataag cagagccagt acagccatca gcggagagag aacaacaagc gcaagcagtg
     6901 acagtgaata gtccatcaac agttttatca tatactgcca tcgcggcatc tcccggtagg
     6961 atactctcag aaggggtgtg gcatagatgg gccctgcctc aacctgccct gaaataacca
     7021 cccgcattga tggcactgcc ctgacaacca catcacggta acagacagcg ttgacaatct
     7081 cctcaacctg accgtattca tcctcttcca tggcgagtat cacctcgttt atctcatgct
     7141 cctcaatgac ccgtatgatc tcatcagcac ttcccagccg cctgatgtca acgtcagact
     7201 gttgttttga gtcaccgctg accgtcagga atccgatcag aagattgccg gcaggcatct
     7261 tctcactcct tatctcactt ataagcctgg ccgccttctc cctgcttccc acgatgagcg
     7321 tcctgaaacc tatttcgcgc cggtggattt tgccggaaac aaccgaggtg attatgagcc
     7381 tcgggatata tgtcagcata aacagaagga ggaagagtac gccaaaagag ctgtagtatg
     7441 ttgagtaatg gcctatgaca tcatcaagga taaggagaaa gaacagtacc actactgcag
     7501 ccagtgttat tgcaagtgat tttccgaatt caagcagccg tgaccggcga agcgggtctg
     7561 aataaaatcc cgccatggca aagacaaaca ggaagaaaag cgggatgagg cttattccga
     7621 gataaaacct ggtacccagt tccatctgaa agtaatcgcc atataacttc gtttcaatga
     7681 aatatttccg gaacaggtag aatagccccc acgccaccga tgagccggtg aagtcagcca
     7741 atatatatga taacctgatg gtgcgggtca attttttcat tatactgatc gggagatatg
     7801 caaagatatc tgttattttt agaccaaatt catgagatga tgattgagga atcttttgag
     7861 gcgaaaggca agagaaaaaa gctgattgag gaactcaggc gcaaggggat caatgatgaa
     7921 agggttcttg aggctatggg taaggttccg cggcatcttt ttatggatga tgcctttctc
     7981 aggcatgcct atcaggacaa ggctttccca atagctgccg ggcagaccat ctcacagcct
     8041 tatacagtgg ctgttcagac tgagctcctc agagtcggga aaagggataa ggtgctggag
     8101 gtggggaccg gctcgggtta ccaggcagca gtgcttgcag agatgggagt gagggtttat
     8161 acaattgaac ggcagaggga gctgtatcgc aaggctcaga ttatgctcgg tgagctgggt
     8221 taccgggtac actttttcct gggtgacggt tatgagggac agccgcaata cggaccctat
     8281 gacggaatca tcatcacggc agccaccgac gaggtgcccg gtgaactgct gaaacagctt
     8341 cggacaggcg gaaggctggt ggtgcccaaa ggaaagcgtg atttgcaggt gatgacgctt
     8401 tacgagcgcc ggggtgaaga tgactatgag atcacaacac acgggaactt tatttttgtt
     8461 cccatgctga ggggaattgc aaacaccaat aacagagagt gacatcatga agatatacag
     8521 agcaatattc tcgcctatcc aggttaatac ctacatcatt accggcaacg gcagtgagtg
     8581 tgtcatcatt gactgtggct gctatggcgg ggccgaagag cagaagcttg aagagatgct
     8641 ttctgccagg gaactgaaac cggtgatgct gctcaacacg cactgccatc tcgaccatgt
     8701 cttcggaaat aggttcatgc ttgacagata cgggctgaga ccgtggtttc atgagggcga
     8761 caggcataac taccggaatg cgcccaaaca ctcactcata tggggtctct ccatggagcc
     8821 gcagcctgat cctgaaggat acctgtctga cgggcaggtg atcagcgccg caggggtgac
     8881 actgacggtt attgccgtac caggccattc accgggaggc gttgcatttt attcagaggc
     8941 tgacgccgtt gtatttaccg gcgatgcact ctttgcagga agcatcggca ggtcagacct
     9001 gccgggcgga gatcatgaac aactgattga aaacatccgg gagaggcttt ttgtcctgcc
     9061 tcctgatacg gttgtttatc ccggccacgg tc
//
LOCUS       DAOPBK010000104         9067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7224_length_9067_cov_4.499112, whole genome shotgun sequence.
ACCESSION   DAOPBK010000104 DAOPBK010000000
VERSION     DAOPBK010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7224_length_9067_cov_4.499112"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PLA17_07200"
     CDS             complement(<1..1314)
                     /locus_tag="PLA17_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPK85239.1"
                     /translation="MAILQDLREKAGPLLAIVIGISLLLFIVSDFFGSNTSQRRKANK
                     YYQLATIDGESVSYQEFEARVQDLADIYKMSGSTEINEEMMQSLREQVWQQMLSEKLM
                     GKTYRQVGLGVSPDEVEMLVFGDDRHPIVRQLFTDPGTGTFNESFLVNFLKATETDDA
                     TKKYWLFFEDQIINDRLNTKLVNLMSKGLYVTGKQSEYENSLHENTVSFSYMVRNYAS
                     IPDSLVKVTSDEIRSYYDKHKDQFKRGASRDIEYITFEIAPSEEDIKETETWAVNEKE
                     NFAAATEVIQYINLTADTRYTGSYLTMDDLPESLRPLAETGDKTTVLGPYLEDETFKL
                     ARIIDIADRPDSVRAAHILLSPNANRTLAQARKEADSLIALVRSGIDFNLLAMANSDD
                     QGSAQVGGDLGWFSEGMMVIPFNNACFSNKKGDIVTAETTYGIHII"
     gene            1618..3594
                     /locus_tag="PLA17_07205"
     CDS             1618..3594
                     /locus_tag="PLA17_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK85240.1"
                     /translation="MNRLIILIVAVTLLCPSVLQAQKRKMERAYDAYNAGSYYEAIDY
                     FKDVYSKTRDKQLKSDMVFMVSECYRLINDPKNAELWYKRAVRSPFPKPEAQYWLAES
                     NKKLGRYPEAIEEFREYRRLVPGDPRTEQQIRSCELAMQWQSAPESYNVEELKEVNTG
                     KSDFSPAYGRDDHGLLWFTSSRDDARGNKEHGATGQSFTDIFETRLDKKGKWSTPVPV
                     DFLNSEFEDGTPSFSFDYSEVWFTRCEAGKRESKGCVIMYSTREGIRWSDPVKVDLLA
                     DSLVAAHPSISPDGQTLYFVSDMPGGFGGKDIYRTTRTSPGSAWSRPENLGADINTRG
                     NELFPFIRENGTLYFASDGHIGMGGLDIFKATPQGGESWSVENMRPPINSPADDFGIT
                     FERDAEKGIFSSSRKGRTYDDLYTFEMPPLLFSITGLVKDEKTALPIQGATVQLIASD
                     GANMQTETGNAGDFKFSLRPEVDYIFLASKKGYLNGKEKETTRGQDKSREFMATILMT
                     PIDRPIELPNIFYDFGKWDLRPESMVSLDKLVETLNDNPTVTIELMSHTDSRDTEEFN
                     LDLSQKRAQSVVDYLIGKGIAADRLTARGYGEASPKVVDDEIMKDAPFLRPGASLTEQ
                     YINSLANDEQKEIAHQVNRRTEFRVLRTDYEPAK"
     gene            3616..4785
                     /locus_tag="PLA17_07210"
     CDS             3616..4785
                     /locus_tag="PLA17_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPK85241.1"
                     /translation="MTDRSRYALRGVSSGKAEVHAAISRVDKGLFPRAFCKIIPDMLG
                     GDPACCNIMHADGAGTKSSLAYIYWKETGDLSVWKGIAQDAVVMNLDDLLCVGVTDNI
                     LLSSTIGRNKNLIPGEVIKALIEGTEEFLARMRDAGIGIWSTGGETADVGDLVRTVIV
                     DSTVTARIALADVIDNANIRSGDVIVGLASFGQTTYESEYNGGMGSNGLTSARHDVFG
                     KYLSGLYPESYDGAIPGELVYSGPMRLTDPTAVPGVNAGRLVLSPTRTYAPVIRKVLD
                     VMRPAIHGMVHCSGGGQTKIMHFIDNLHVVKDNLFPVPPLFELIQKSSGTPWDEMYRV
                     FNMGHRMELYVDEADAAAIIEIASSFNLDARVVGRCEPAATRRLTISTDQGKFVY"
     gene            5144..6229
                     /gene="prfA"
                     /locus_tag="PLA17_07215"
     CDS             5144..6229
                     /gene="prfA"
                     /locus_tag="PLA17_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPK85242.1"
                     /translation="MANNLILDRLTGIKKRFVEVGELLASPDVMSDMKRYIKLNKEYK
                     ELKPVVEAYEKYKSSIDNLESAKELLSSEKDEEMREMAKDEIDMLLQSIPEMEEEIRY
                     LLLPADPEDEKNAVMEIRAGTGGDEASIFAGDLFRMYSKYIEEQGWKIDLNYYTEGTA
                     GGYKEIVFNVIGDGVYGILKYESGVHRVQRVPQTETQGRVHTSAATVAVLPEAGEFDI
                     EIRNEDIRKDTYCSSGPGGQSVNTTYSAIRLTHIPTGVVVTCQDEKSQIKNLEKAMRE
                     LRTRLYNLEYQKYLDEISKKRKTMVSTGDRSAKIRTYNYPQGRVTDHRINTTLYNLPS
                     ILDGNIQELIEKLQMAENAERLKAHDL"
     gene            6241..7068
                     /gene="pyrF"
                     /locus_tag="PLA17_07220"
     CDS             6241..7068
                     /gene="pyrF"
                     /locus_tag="PLA17_07220"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPK85243.1"
                     /translation="MTARELFQHILKKKSFLCVGLDPEISKLPACLAELEEPIFEFNR
                     QIIDATHRYAVAYKPNFAFYESYGARGMAALDKTVRYINKLDTNIFIIADAKRGDIGN
                     TSKMYARAVFGEMPFDAVTVAPYMGEDSVTPFLSYGGKWAIILALTSNRGADDFQYHT
                     DKGQRLFERVLTLSQRWGTIDNLMYVVGATRAEMLADIRKIVPDHFLLVPGVGAQGGS
                     LEEVARYGMNNQCGLLVNSSRGIIYADSSDNFADVAGEKAREMQEEMALYLADRFKI"
     assembly_gap    7225..7324
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7524..8846
                     /locus_tag="PLA17_07225"
     CDS             7524..8846
                     /locus_tag="PLA17_07225"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPK85244.1"
                     /translation="MKEEFIHWLWKNQFFDGRSLCDREAGSIGVITPGVYNRDSGPDF
                     FNTRLIIGGTEWAGNTEIHVNASDWYRHGHHIDHAYDNVILHIVYNEDADVYTASGRR
                     LITIRLVFDPVLWENYLDFMNNPSPLPCSGLIGLTDGFRIKHWLWSIAVERLERKHDD
                     IREILSKTGNDWEETLYRLVTRYFGFRVNTDPFGMLAARLPLKIIRKHSDQLIQVEAL
                     LYGTAGLLDEALFREAVNDSYYLLLLREYRVLRAKYSLQPVDGWLWKFHRLRPANFPT
                     VRLSQLAALLSHSDGLFSRVLGCSDRESLRALLSVSASSYWNNHYQFGREVPPVAGRA
                     GRQSADLLIINAIVPLLFVYGKVRQQQEWCDRAVEILDSLPPEKNSVVTDFTRAGLKP
                     ESAFASQALLELRNMRCRYHRCLDCTIGSSLIAMGQKIRRSDSLFLEP"
BASE COUNT         2207 a         2253 c         2342 g         2165 t
ORIGIN      
        1 gatgatatgt atgccgtagg tcgtctctgc cgtcacaatg tcaccctttt tattactgaa
       61 gcaggcatta ttgaagggta tcaccatcat cccttcgctg aaccatccga ggtcgcctcc
      121 tacctgtgcc gagccctggt catcggagtt ggccattgcc agcaggttga agtcaatccc
      181 tgacctgacg agcgcaataa ggctgtcagc ttcttttcta gcctgtgcaa gggttctgtt
      241 tgcgttggga gagagaagaa tatgtgctgc cctgacagaa tcaggacgat cggctatgtc
      301 aattatgcgt gccagtttga aggtttcgtc ctcaagataa ggcccgagaa cggtagtttt
      361 gtcgcctgtc tcagccagtg gcctcaggct ttcaggaaga tcatccattg tcaggtaaga
      421 gccggtatac ctggtgtcag ccgtcaggtt gatatactga atcacttcag ttgctgcagc
      481 gaagttctcc ttctcattga cagcccaggt ctcggtctcc ttgatgtctt cctccgaggg
      541 agcaatctca aaggttatat actcaatatc cctggaggca ccacgcttga attgatcctt
      601 gtgcttgtcg tagtagctcc tgatctcgtc agaggtaacc ttcaccagac tgtcaggaat
      661 agatgcatag ttgcgcacca tatagctgaa gctgacagtg ttctcatgga ggctgttctc
      721 atactccgac tgtttgccgg tgacatagag accctttgac atcaggttga ccagcttggt
      781 attgagcctg tcattgatta tctgatcctc gaagaagagc cagtattttt ttgttgcgtc
      841 atcagtctcg gtggccttca ggaaattaac aaggaatgat tcattgaagg tgccggtgcc
      901 ggggtcagta aaaagctgcc tgacaatagg atggcggtcg tcaccaaaga cgagcatctc
      961 cacctcgtca gggctgacgc caaggcccac ctgccggtag gttttgccca tcagcttctc
     1021 tgagagcatc tgctgccata cctgttccct caggctctgc atcatttctt cattgatttc
     1081 tgtagagcca gacattttgt atatatctgc aaggtcctga acacgggctt cgaactcctg
     1141 gtatgacacg gactccccgt caatagtggc aagctgataa tatttgtttg cctttcggcg
     1201 ctgggaagtg ttgcttccga aaaaatcact cactataaag agaagcagtg aaatacctat
     1261 cacaattgcc agaagcggcc ctgctttctc tcttaaatcc tgaagtattg ccatctgaat
     1321 actaattaaa caatgaatct tacctcttga aaaatacagg cgacaaatat aacaaaattc
     1381 agttaaacaa aaggatgggc ctccctctca ttttatttaa cttccgaaga aataccatga
     1441 gcttctgatt atccggcaaa cggttgattt attaccatta attatggttt gcaataccct
     1501 ctgttgaaga gggcattatt tttgctgcag attacgggtg ccggtaatta atgaaacggc
     1561 cggacgttaa cattaataac aatcattact tttgtacaaa acagtatcgt gtccggaatg
     1621 aacagattaa taatattgat agttgctgtt acccttctct gcccatctgt gcttcaggca
     1681 cagaaacgga agatggaacg cgcatacgac gcatacaacg ccggaagcta ttatgaggca
     1741 atagactatt tcaaggatgt ctactccaag actcgtgata agcagctgaa atctgacatg
     1801 gtatttatgg tgtcagagtg ctaccgcctc atcaatgacc cgaaaaatgc cgaactgtgg
     1861 tacaaaagag ctgtcaggtc gcctttcccg aaacctgaag cgcagtactg gctggcagag
     1921 agcaacaaga agctgggacg gtatcccgag gccatagagg agttccgtga ataccgcagg
     1981 cttgtgccgg gagatccccg taccgagcag cagatacgct catgcgagct tgccatgcaa
     2041 tggcagtcag cgcctgaatc ctataacgtc gaagaactga aggaagtcaa tacagggaaa
     2101 tctgatttca gtcccgccta tggccgcgat gatcatggtc tgttatggtt cacctcctcg
     2161 cgcgatgatg ccaggggtaa caaggagcac ggggcaacag gacagagctt tacggatatc
     2221 ttcgagacca ggcttgacaa gaaaggcaag tggagcactc cggtgcctgt tgatttcctg
     2281 aacagtgaat tcgaggacgg aaccccctca ttctcatttg actactcaga ggtgtggttc
     2341 acgcgctgcg aggctggtaa gcgcgagagc aagggatgtg tcataatgta ttcaacccgc
     2401 gagggcatcc gctggagtga cccggtaaag gttgatctcc tggcagactc ccttgttgca
     2461 gcccatccct caatatcacc tgacgggcag actctctatt ttgtctccga tatgccggga
     2521 ggatttggtg gcaaggatat ataccgcact acacgaacct cgccgggaag tgcatggtca
     2581 cgcccggaaa acctgggcgc tgatatcaat acccggggca acgaactctt ccccttcata
     2641 cgtgaaaacg gaacgctcta tttcgcctcc gacggacaca tcggcatggg cggactagat
     2701 atctttaagg ctacgccgca gggcggcgaa agctggagcg ttgagaacat gaggccgcca
     2761 atcaacagcc cggcagacga ctttggcatc accttcgaga gagatgcaga gaagggtatc
     2821 ttcagttcgt cacgcaaggg gaggacttat gatgacctct acaccttcga gatgccgcct
     2881 ctgctcttca gcatcacggg cctcgtcaag gatgaaaaaa cggctcttcc catacaggga
     2941 gccaccgtgc agctgatagc cagcgacggc gccaatatgc agacagagac gggtaatgca
     3001 ggcgacttca aattcagcct gagaccggag gttgattaca ttttccttgc ctccaagaag
     3061 ggctacctca acgggaagga aaaagagacg acaagaggac aggacaagag ccgcgaattc
     3121 atggccacca tactgatgac ccccatcgac aggcctatcg agcttccgaa tatattctat
     3181 gacttcggca aatgggacct caggcctgaa tcaatggttt cacttgacaa gctggtagag
     3241 accctcaacg acaatccgac ggtgaccatc gagcttatgt cgcacaccga ctcccgtgat
     3301 accgaggagt ttaacctcga tctgtcacag aagagggcac agtcggttgt tgactacctg
     3361 ataggcaagg gaatagctgc tgacaggctt actgccagag gctacggcga agcgagcccg
     3421 aaagtggtcg atgatgagat catgaaggat gctccgttcc tgcgcccggg tgcatcgctt
     3481 accgagcagt acatcaacag cctcgccaac gatgagcaga aagagatagc ccatcaggtc
     3541 aaccgtcgta ctgagttcag ggtgcttcgg accgactatg aacctgcaaa ataattgaga
     3601 accggggacg gggttatgac agacagatca cgctacgcat tgagaggagt ctcttcagga
     3661 aaggctgaag ttcatgctgc aatcagccgt gttgacaagg gcctctttcc ccgggcattc
     3721 tgcaagataa tacctgacat gctgggaggt gatcctgcct gttgcaatat aatgcatgct
     3781 gacggcgccg gaaccaagtc gtcactggca tatatttact ggaaggagac gggcgacctg
     3841 tcggtctgga aggggatagc ccaggatgcc gtggtaatga atcttgacga cctgctatgc
     3901 gtaggagtaa ctgataatat tcttctatca tcaactatag gaaggaacaa gaaccttatt
     3961 cccggtgagg tgataaaagc cctgatagaa ggcacggagg agttccttgc ccggatgagg
     4021 gatgccggca tcgggatatg gtcaacggga ggagagacgg ctgatgtggg tgacctggtc
     4081 cgtacggtga ttgttgactc cacggtgaca gcccggatag ccctggctga tgtcattgac
     4141 aatgcgaaca tcaggtcggg ggatgttatt gtggggcttg catcgttcgg gcagacaaca
     4201 tatgagagtg agtacaacgg tggtatggga agcaacgggc tgacctctgc acggcacgat
     4261 gtgtttggca agtacctttc cggcctttat cccgagagct atgacggtgc tattcccggg
     4321 gaactcgttt acagcggacc gatgcggctt accgatccca cagcggtgcc gggcgtcaat
     4381 gcaggcagac tggttttatc acccacacgc acctatgcac cggtgataag gaaggtattg
     4441 gatgttatgc ggcctgccat acacgggatg gtgcactgtt caggcggcgg gcagaccaag
     4501 atcatgcatt tcattgacaa cctgcatgtg gtcaaggata atcttttccc ggtaccaccg
     4561 ctgtttgaac tgattcagaa gagttccggc acaccgtggg atgagatgta ccgtgtgttc
     4621 aacatgggtc accgcatgga gctttatgtt gatgaggcag atgctgccgc gatcattgaa
     4681 attgcttcat cattcaatct tgatgcccgt gtagtagggc gctgtgagcc tgccgcaacc
     4741 cgcaggctta caatcagtac tgaccagggg aaatttgttt actgatcctc ttcagcgcat
     4801 aactgcccgc aaccaaaaac cccacaccac aacccttttg ccataatatc ctgtacccaa
     4861 cccactgcca cttaattact aattgctcat tgccaattgc caattcagct tcgctgcatt
     4921 ttctgtcgtc gcccggcata gttttgcaaa gcaacacttt tgctcgtcgt ggcaaaatcc
     4981 gagtaagctc gtcttctgct caccgactta ccgcaaaagt tcaagcaagc ttgactctgc
     5041 actcactcct aagaaaattg ctcattgctc attgttcatt gttaattgct aattgccaat
     5101 tgtcttatat tgattacatt tgcaccctga aattttgcat ctgatggcaa acaatcttat
     5161 tcttgacagg ctgactggca taaagaagcg ttttgtggag gtgggtgagc ttcttgcctc
     5221 ccctgacgta atgtctgata tgaagcgcta tataaagctt aacaaggaat acaaggagct
     5281 caaacctgtg gttgaggcgt atgagaaata caaatcctcc attgacaatc tcgaaagtgc
     5341 aaaggaactt ctctcctcgg aaaaggatga ggagatgcgt gagatggcca aggatgagat
     5401 agatatgctt ttgcagtcta ttccggagat ggaggaggag atcaggtatc ttctcctgcc
     5461 tgctgacccg gaggatgaaa agaatgctgt catggagatc cgtgccggta cagggggtga
     5521 cgaggcaagt atctttgcgg gggacctctt ccgcatgtat tcgaagtata tagaggaaca
     5581 gggctggaag attgatttga actactatac cgaaggcact gcaggagggt acaaggaaat
     5641 agtattcaac gtgataggtg acggtgtcta tggtatcctg aagtatgagt cgggtgtcca
     5701 ccgcgttcag cgggtgcctc agaccgagac acagggccgc gtccacacat cggcagcaac
     5761 ggtggcggtg cttccggaag ccggcgagtt tgacatagag atccgcaacg aggatatccg
     5821 caaggatact tactgctctt cgggtcccgg cggacagagc gtcaacacta cctattcggc
     5881 aatcaggctg acacatatcc ccacaggggt cgtggttacc tgccaggacg aaaagtcaca
     5941 gatcaagaac cttgagaagg cgatgaggga gcttcgcact cgtctctata accttgaata
     6001 ccagaagtac ctcgacgaga tctcaaagaa gcgcaagaca atggtgtcta cgggcgacag
     6061 gtcggccaag atacgcacat acaactatcc gcagggacgg gttactgacc accgcatcaa
     6121 cacaaccctt tacaacctgc cgtcaattct tgacgggaac atccaggagc tgattgaaaa
     6181 gctgcagatg gctgagaacg ctgagcgtct gaaggcgcat gacttataaa atatgatatc
     6241 atgaccgcga gggaattatt ccagcatatt cttaaaaaga agtccttcct ctgtgtcggt
     6301 ctcgatcccg agatctccaa actgccggca tgcctggcag agctggaaga accgattttt
     6361 gagtttaaca gacagataat agatgccacc cacaggtatg cggtggccta taagccaaat
     6421 ttcgcctttt atgagtccta tggcgcaaga ggcatggcag cactggacaa gacagtgagg
     6481 tatatcaaca agcttgacac aaatatcttc atcatagctg acgccaagcg gggtgacata
     6541 ggcaacacct caaagatgta tgccagggcg gttttcgggg agatgccgtt tgacgcggtg
     6601 accgttgcgc catacatggg agaagattct gttacgccgt ttctctccta cggaggcaaa
     6661 tgggcaatca ttcttgcgct tacctcaaac aggggagccg atgatttcca gtatcacacc
     6721 gataaaggtc agcggctttt tgagcgggta ctgactctct ctcagcggtg gggcaccata
     6781 gacaacctga tgtatgttgt aggtgcaaca agggctgaga tgcttgctga cattcgtaag
     6841 attgtgccgg atcatttcct tcttgtgccc ggtgtaggtg cacagggagg cagccttgag
     6901 gaggttgccc ggtatggcat gaacaatcag tgcggcctgc tggtaaactc atcgcggggt
     6961 ataatctatg cggattcatc cgacaacttt gccgacgttg ccggcgaaaa agcccgtgag
     7021 atgcaggagg agatggccct gtacctggca gacaggttta aaatctgacc agccggcatc
     7081 tggcactgcc tgccggcttt aaatgactta tttactgcac attgcgcact gacgcctctc
     7141 tccgctgcaa taagcctttc cgcttgcata ctgacgcctc tctccgctgc agtatgccgt
     7201 tccgtttgcg cactgatgcc tctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnctgtat taaaccgtca cagccggggc tttaatcagc ctcttcacag ggtatggagt
     7381 taaggagtta ttatctccgt cacatcaatt tttgattgct tcgtcggatt attatggggc
     7441 tccccgtaac acctcctttt ttttgtgctt ttggaacatt gttattaaat ttgataagct
     7501 gcatttctta aataataccg accatgaaag aagagttcat ccactggttg tggaaaaacc
     7561 agttctttga cggcaggagt ctgtgcgaca gggaagccgg ctccattggg gtgataaccc
     7621 cgggagtgta caaccgtgat tcaggacctg atttttttaa caccaggttg ataataggag
     7681 gtaccgagtg ggccggcaac actgagatac atgtcaatgc ctccgactgg taccgtcacg
     7741 gccatcacat tgatcacgct tatgacaatg tcatcctgca cattgtatat aatgaggatg
     7801 cagacgtcta tactgcttcc ggccgcaggc tgataaccat ccgcctcgtt tttgatcctg
     7861 tattgtggga aaactacctc gatttcatga ataatccttc tccgctgccc tgcagcggac
     7921 tgataggact tactgacggt ttcaggatca agcactggct gtggtcgatt gccgttgaac
     7981 gtctcgaaag gaagcatgac gatatcaggg agattctgtc aaagaccggc aatgactggg
     8041 aggagactct ttaccggctg gtaacccgtt actttggttt cagggtaaac actgatccct
     8101 tcgggatgct tgccgcccgc cttcctctta aaatcataag gaagcactct gaccagctca
     8161 tacaggttga ggccctgctg tatggcacag cggggttgct tgacgaggct cttttcaggg
     8221 aggcggtgaa cgacagttat tatctcctgc tgttgaggga gtaccgtgtg cttcgcgcca
     8281 agtattcact tcagcccgtt gacggctggc tctggaagtt tcacaggctg cggcccgcca
     8341 atttcccgac tgtcaggctc tcccagctgg cggcgctgct cagccacagt gatggtctct
     8401 tctcgagggt actcggctgc agtgaccgcg agagcctccg tgcactgctg agcgtcagcg
     8461 cctcttctta ctggaacaat cactaccagt tcggacgcga ggtgccgccg gtggcgggtc
     8521 gcgccggcag gcagtctgca gacctgctca tcatcaatgc cattgtgcca ctgctcttcg
     8581 tctacggcaa ggtcaggcaa caacaggagt ggtgtgacag ggcagtggag atacttgact
     8641 ctctgcctcc ggaaaagaat agcgtggtga ccgacttcac ccgggcgggg ctgaagccgg
     8701 agtcggcctt tgcttctcag gctctgctgg agctgagaaa catgagatgc aggtatcaca
     8761 ggtgtctcga ctgcaccata gggtcatcgc tcattgccat gggtcagaaa atcaggaggt
     8821 cagactcgct ttttctggag ccctgacacg cttccgctag tcaggtattc acgtcccggg
     8881 gcagatgaag gctcctccgg tgcattactc cccctcccgg gctggcaagc gctcttccgg
     8941 catattgccc ctccctcccg gcgcaggtaa actcttctcc agcatattac cccgttcccg
     9001 ggacaagtaa ctgctcctcc ggtgcattac tccccctccc gggctggcaa gcgctcttcc
     9061 ggcatat
//
LOCUS       DAOPBK010000105         9031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7254_length_9031_cov_5.160316, whole genome shotgun sequence.
ACCESSION   DAOPBK010000105 DAOPBK010000000
VERSION     DAOPBK010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7254_length_9031_cov_5.160316"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PLA17_07230"
     CDS             complement(<1..136)
                     /locus_tag="PLA17_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HPK85245.1"
                     /translation="MSSRFPSRLLEEAVNEFASLPGIGRKTAFRLVMHLLRRDSGEVR
                     R"
     gene            243..1709
                     /locus_tag="PLA17_07235"
     CDS             243..1709
                     /locus_tag="PLA17_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPK85246.1"
                     /translation="MTPTLILLVFTAYTLLLFAITFVTARRANHQSFYIGNKASPWYV
                     VAYGMIGASLSGVTFMSVPGWVGETQFSYMMVAFGYFFGYVVIALVLLPLYYRLQLTS
                     IYGYLEQRFGFWSYKTGAGFFILSRVLGASLRMFLVINVLQIFVFDAWNVPFWVNVLI
                     FIALVIIYTLKGGIRTIIWTDTLQTTFMLLAVVLSVVFISRELGEPVQKLLKNLFESD
                     TSRMVITDWDHPRHFIKQFFSGMFITITMTGLDQEMMQKNLSIRHLKDAQKNMFTFST
                     INLVVVFMFLVLGAFLLFFARARNINVAMSDDLFPTIAFRYLSPAAGIVFLIGLISAA
                     FPSADGALTSLTTSFSIDFLGLEKKPEMDYAKKTRIRYTVHLSFALIFFICILVFRQM
                     NDRAIIDKLFTIAGYTYGPLLGLYTFGLFTKRRVTDRFTPWIAIISPIICYFLHLYSE
                     ELFNGYRFGFEMILVNGFLTFMGLLIFSRKGSKGVPEA"
     gene            complement(1686..2453)
                     /locus_tag="PLA17_07240"
     CDS             complement(1686..2453)
                     /locus_tag="PLA17_07240"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HPK85247.1"
                     /translation="MVRRTCIFFISLFLIAENVAVSQDTVTVPLHVRAGFDISGPVMK
                     LVAKELISYGIAASADINSSIAATAGLRYSSYHSSDVLYDFMSRGLSFTAGTDYNFIK
                     ANVSQGRHSAGIGLRYGLSFYREETPKLRYTNEWGTGETSVAAESHTGHFLEITPGVR
                     TELFRGVTIGWNLYMRLLLSAGAGKDLKPVWMPGYGPGTSGMTVGAEYYVSISIPYRK
                     IKVYIKPKVRDTEEEEGEATETGSGTGTGLSGFRNPL"
     gene            complement(2422..2889)
                     /locus_tag="PLA17_07245"
     CDS             complement(2422..2889)
                     /locus_tag="PLA17_07245"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HPK85248.1"
                     /translation="MKRLSVISFIAIMATLVMFLPSCSVQPCYEETNPLLKTVLLASG
                     TGEAAKADSLRVYTYRETDTLRFINLKGASSFSVPLDPASDESEFLITLNGITDTAVI
                     RYSRKPHLVSPECGYTIISELTGLKTTHNIIDTLIIENKSVNLDGQKNLHLFY"
     gene            complement(3089..4942)
                     /locus_tag="PLA17_07250"
     CDS             complement(3089..4942)
                     /locus_tag="PLA17_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPK85249.1"
                     /translation="MEVTRTFDLIDQACKVYPREVMFGGKPDKGWITYSTEAIRELTD
                     LFSCGMMAIGYTRGDHIATITPNKAEWNIVDHGLAQAGMIHVPIYPTIGNEEYAYILD
                     HSGTKAVIIGNKQIYNKILPVASNIPAIRAIYSFDQVNDLMTIDDIIEEGRKNREKFM
                     GELSAIRDSIKPSDMVTIIYTSGTTGNPKGVMLSHNNLISNAIATSAAHEFGLGFRAL
                     SFLPLCHVYERMMNYHFQYKGLSIYYLENMGAIAETMREIKPHLANTVPRLLEKIYDN
                     IISRGKNLPWLKKQIFFRAVNLGLKFRLRGNTWFYRFRLGIARKLVFNKWQDALGGEL
                     RVLVSGGAALQSRLERIFWAAGIPVIQGYGLTETSPVIAVNPLRIPEIKFETAGPVLD
                     GVQVKIADDGEILCKGPNVMIGYYREPEMTAEVIDSDGWFHTGDIGRIEDGRFLRITD
                     RKKEMFKLSAGKYISPQVIENKLKESIFIEQAMVIGENKKFASALISPNFTYLHDWCS
                     IHRIQYRDHTDLIEMHEVIERFAREVREVNKSLGEFEQIKRFRLVTEEWTPQTGELSS
                     TLKLRRNILNSRYQNIINEIYSIKEKGNMVDKVVEVIRNGVSGILKNLPKF"
     gene            complement(5100..7733)
                     /gene="alaS"
                     /locus_tag="PLA17_07255"
     CDS             complement(5100..7733)
                     /gene="alaS"
                     /locus_tag="PLA17_07255"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPK85250.1"
                     /translation="MKANEIREEFLSFFRSKEHRIVPSAPMVIKNDPTLMFTNAGMNQ
                     FKDIFLGNQPAKYKRVANSQKCLRVSGKHNDLEEVGLDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWEFLCDRLGIPADRLYATVFEGDPVENVPRDEEAATWWAKCFGGAENHILD
                     GSKKDNFWEMGETGPCGPCSEVHVDIRSESERAATPGHLLVNTGHPRVIEIWNLVFIQ
                     YNRRAGGELELLPDRHVDTGMGFERLCMVIQNKQSNYDTDIFQPLLAASAEVTGKPYG
                     EKRDWDIAHRVIADHLRTVSFSVADGQLPSNNRAGYVIRRILRRAVRYGYTSLGMTGP
                     FIYKLLPALVETMGGHYPELKEQQDIISKVIHEEEQSFLRTLDKGLRMIERRVEALRK
                     EGKSILPGADAFELFDTYGFPVDLTQLILRENRMTLDEEAFRSELNAQKERSRDDAAV
                     STDDWVVIRETESTIFTGYDRTQEQVRIARYRKVTSRGRQAVQLVFDRTPFYAESGGQ
                     TGDRGYIEAGSEKIIITDTVKENNLTIHIASALPADPSLTFRAIVDAEARQDTANNHS
                     ATHLMHHALREVLGKHVEQKGSLVTPDRLRFDFSHFRQVTPEELSAVEEKVNSMIMQN
                     IISEVHDHIPMEDARAMGAIALFGEKYGDRVRVVRFGDSVELCGGTHVAQTSRIGLFK
                     IVSEGAIAAGVRRIEAVTGRKAVSYINEKLSILGEVTALLHSTGNPVANISKLMAENA
                     ALKASIEKLQAAAALSARNELAARAETAGNCRIYSGLLEETSADTLKNIAHQIRQTDS
                     SSIIVIGSAYEGKASLVVGLGNQAAGSGHLNAVEIIKAVSGEINGGGGGQPFLATAGG
                     KNPEGLPGAVARAVEMAKKML"
     gene            7850..8806
                     /locus_tag="PLA17_07260"
     CDS             7850..8806
                     /locus_tag="PLA17_07260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPK85251.1"
                     /translation="MLKRRNYVFDHTNLQFLQHRRNRGERILRFALWFSLSIVVTFFY
                     SIIFHRLFGSPKEEMLRQQVEELKLRYTMLEKQIDYSTAAVTELRSSDNTLYRAVLDM
                     PAIPADISEGGSGGTNRLADLTGYLNSDLLMKVRSKFDDLKTQTNIQYNSLAELTARA
                     SEWKEMWEHLPYIRPVNVTLRLGDSMGFREKHPVLGSPRWHFGQDFCCPIGTEVYATG
                     AGIVAYAGNQRDGFGIKVVIDHGYGYRTIYGHLSEFNVRQGQRVTRGDFIGLSGNTGT
                     STGPHLHYQIDLFGAHVNPLWYFQDNLTEEEYFMMLEYLSKP"
BASE COUNT         2028 a         2293 c         2213 g         2497 t
ORIGIN      
        1 aacggcggac ttctccgctg tcacgcctca gcaggtgcat cacaagcctg aaggcggtct
       61 tgcggcctat gccggggagg gaggcaaact cattcacggc ctcttcgagc agtcttgaag
      121 ggaaacgaga tgacataatg cgactttgcg gcaaaaataa tccaatattt gctgcaaacc
      181 tgactacaat tgacttttat ctgcgatctt tttagtaatc ttgcggacaa ttttccggaa
      241 ttatgacacc tacactcatc ctgttggttt ttacagccta tacgctcctg ctgtttgcca
      301 tcacctttgt tactgcacga agagccaatc atcagtcatt ctatataggt aataaagcat
      361 ctccatggta tgttgtggct tacggcatga tcggagcctc cctgtcaggg gtgaccttca
      421 tgtcagtgcc cggatgggtg ggagagaccc agttctcata catgatggtg gcgttcggat
      481 atttcttcgg atacgtggtt attgccctcg tactcctgcc gctctattac cgtctgcaac
      541 ttacctccat atacggctac ctggaacagc ggttcggttt ctggtcatac aagaccggag
      601 ccggattctt tatcctttca agagtcctgg gcgcatcact ccggatgttc cttgtgataa
      661 acgtcctgca gatttttgtg tttgacgcgt ggaatgttcc cttctgggta aacgtgctga
      721 tattcattgc tctggtgata atatacactc tcaagggagg gattcggacc attatctgga
      781 cagacacact ccagaccacc tttatgctgc ttgcagttgt gttgtcggta gttttcatca
      841 gtcgtgaact gggagagcct gtacagaagc ttctgaagaa tctgtttgag agtgacacgt
      901 cacggatggt gattaccgac tgggatcacc cgcgtcattt catcaagcaa ttcttcagcg
      961 gcatgttcat aaccatcacc atgaccgggc ttgaccagga gatgatgcag aagaacctca
     1021 gcatcaggca tctgaaagat gcacagaaga atatgttcac cttcagcacc atcaatcttg
     1081 ttgtggtttt catgtttctg gtccttggcg cattcctgct cttctttgcc agggccagga
     1141 acatcaatgt ggcaatgagt gacgatctct ttccgacaat tgctttcagg tatctcagtc
     1201 ctgctgccgg cattgttttc ctgatcggac ttatatcagc tgcattcccc agtgcagacg
     1261 gggctttgac atcactgaca acctccttca gcatagactt cctcggcctt gagaaaaagc
     1321 ctgagatgga ttatgccaaa aaaaccagga tcaggtacac cgtacactta tcatttgcac
     1381 tcattttctt tatctgtata ctggtcttca ggcagatgaa tgacagggcg ataattgaca
     1441 agctgttcac catagccgga tacacctacg gtccgctgct cggtctgtac actttcggtc
     1501 tcttcaccaa aagaagggta actgaccgct ttactccatg gatagctatc atctcaccga
     1561 ttatatgcta ttttctgcat ctctattcgg aagaactctt caacggttac aggttcggct
     1621 ttgagatgat actggtaaac ggttttctta catttatggg attgctgata ttcagcagga
     1681 aggggtcaaa gggggttcct gaagcctgat aagcccgtac cggttccgct gccggtctct
     1741 gttgcctcac cttcttcctc ctccgtatcg cggactttgg gtttgatata aaccttgatt
     1801 ttcctgtagg ggattgatat tgacacataa tattctgcac caactgtcat accggatgta
     1861 cccgggccgt agcccggcat ccataccggt ttgagatcct tccctgctcc ggcactcaac
     1921 agaagtctca tataaaggtt ccagcctatt gttactcccc tgaagagttc agtacggaca
     1981 ccaggagtga tctccaggaa atgacccgta tggctttccg cagctacgga tgtttctccg
     2041 gttccccatt catttgtgta cctgagcttc ggggtctcct ccctgtagaa gctgagcccg
     2101 tacctgaggc ctatgcctgc actgtgcctg ccctgtgata cattggcctt gataaagttg
     2161 taatctgtac cggctgtgaa gctgagaccc cggctcatga agtcgtacaa cacatctgat
     2221 gaatggtacg atgagtacct taagcctgcg gttgccgcta ttgatgagtt gatgtcagcc
     2281 gatgcggcaa tgccatagct gataagctct ttggcaacaa gtttcatgac cggtccggag
     2341 atgtcgaatc ctgccctcac gtgaagcggc acggtaaccg tatcctgtga aacggcaaca
     2401 ttctctgcaa tcagaaacag actaataaaa aagatgcagg ttcttctgac catcgaggtt
     2461 cacggattta ttctctatga tgagggtgtc aattatatta tgggttgttt tcagcccggt
     2521 taattcgctt attattgtgt atccgcactc cggcgaaaca aggtgtggct tccggctgta
     2581 gcgtatcaca gccgtatcgg tgataccgtt gagggttatc aggaactctg actcgtcgct
     2641 tgccgggtca agaggcacgg agaatgatga tgctcccttc aggttgataa atctgagagt
     2701 atcggtttcc ctgtatgtat ataccctgag tgagtctgcc tttgctgcct cgcccgttcc
     2761 gctggccaga agaacagttt taagtaacgg attcgtctcc tcgtagcatg gctgcaccga
     2821 gcaggaggga aggaacataa ccagggttgc catgatggca ataaagctta tgacggagag
     2881 cctcttcata acggaataca aaggtataaa aataatatgg agtcaatcat tgaacagcat
     2941 ccccttacag atgctgcagt gcagtttttc cggtgatcct gtaaggagga aatcattgaa
     3001 tgatatcccc tcacagagtg ccggaaaact ggccgggctg gacttggcgg agccggtcag
     3061 gcaggtctga ggtggcagat gccggctgtc agaatttcgg caggttcttc aggatgccgc
     3121 tgactccgtt gcggattact tccacaacct tgtcaaccat gttacccttc tccttaatag
     3181 agtaaatctc atttatgatg ttctgatacc tgctgttcag gatattcctg cgcagcttga
     3241 gggtggatga gagttcgcct gtctgggggg tccactcttc tgtcaccagc ctgaaccgtt
     3301 ttatctgttc aaactctccg aggctcttgt tcacctccct gacttccctg gcaaaccgtt
     3361 ctatcacctc gtgcatctct atcaggtcgg tgtggtcacg gtactgaatc ctgtgtatcg
     3421 agcaccagtc gtgcaggtag gtgaagttgg gtgatatcag ggcgctggca aactttttgt
     3481 tttcacctat caccattgcc tgttcgataa agatcgattc cttcagttta ttttcaatca
     3541 cctggggaga gatgtatttg ccggctgaca gtttgaacat ctcctttttg cggtcagtga
     3601 tccggaggaa cctgccatcc tcaattcttc cgatatcgcc ggtatggaac cagccgtcgg
     3661 aatcaatcac ctcagctgtc atctccggct ccctgtagta tccaatcatt acattgggcc
     3721 ccttgcagag gatctcaccg tcatcggcta tcttcacctg caccccgtca agaacaggtc
     3781 ctgctgtttc aaactttatt tcagggatgc gcagtggatt tacagcgatg acaggagatg
     3841 tctccgtgag tccgtaaccc tgtataacag gtatgccggc agcccagaaa atgcgttcaa
     3901 ggcgcgactg cagggcggcg ccgccggaga ccagaacgcg tagctcacca cccagcgcat
     3961 cttgccattt gttaaaaacc agcttgcggg ctatgccaag cctgaaccgg taaaaccagg
     4021 tgttgcccct gagcctgaac ttaagtccca ggttaactgc ccggaagaat atctgttttt
     4081 tgagccaggg caggtttttt cctctgctta taatgttgtc ataaatcttt tccagcagcc
     4141 ttgggaccgt atttgccagg tgtggcttga tctcacgcat tgtttctgca atggcaccca
     4201 tgttctcaag gtagtatatg cttaatccct tgtactggaa gtggtaattc atcatccgct
     4261 catagacatg acagagaggc aggaagctga gcgccctgaa acccagtccg aattcatgcg
     4321 cggcactggt tgctatagca ttggagatca aattgttgtg agacagcata acccctttgg
     4381 gatttccggt ggtgcctgaa gtatatataa tggtcaccat gtcagagggt ttgatggagt
     4441 ccctgatagc tgacagttca cccatgaatt tttcccggtt ctttctgccc tcttcgatga
     4501 tatcatcaat tgtcatcaga tcgttgacct ggtcaaagga gtagatcgcc ctgatggcgg
     4561 ggatatttga cgctacaggc aaaatcttgt tgtaaatctg tttgttgccg atgatcactg
     4621 cctttgtccc ggagtgatcg agaatgtatg catactcctc attgccaatg gtagggtaga
     4681 tgggtacatg gatcatgccg gcctgtgcca gtccgtggtc tacaatgttc cattcggctt
     4741 tgttcggggt gatggtagcg atgtgatcac ccctggtgta accaatggcc atcatgccac
     4801 acgaaaacag gtcggtcagt tcccttattg cctcggtaga gtaggtgatc caacccttgt
     4861 cgggtttacc accgaacatg acttcccgcg gatagacctt acacgcctgg tcaatcaggt
     4921 caaacgttct ggtaacttcc atcagaatag attttagatt tgcaatacaa gattaggaat
     4981 ttcgaacaaa aaaaacaaga attggggcga tcaaattaat ggcggcctaa tgatttgatc
     5041 tatagtctgg tactggatcc tgttttttgc gaatgtttcg aacggcgaaa aaatctctcc
     5101 taaagcattt tttttgccat ctccacggcc cttgccactg ccccgggaag tccttcagga
     5161 tttttgcctc cggcggttgc caggaatggc tgtccgcctc ctcctccgtt tatctcgccc
     5221 gagacagcct tgatgatttc gacggcattg agatggccgg aaccggctgc ctggtttccc
     5281 aatcccacca ccagggaagc tttgccttcg tatgccgaac cgatcacgat aatgctgctg
     5341 ctgtcagtct gtctgatctg gtgagcgata tttttcagcg tatcagcact ggtctcttca
     5401 aggagacctg aatagatacg gcagtttcct gcagtttcag ctcttgccgc cagttcattg
     5461 cgggctgaaa gtgctgctgc agcctgcagt ttttcaatag aggctttgag cgcagcattc
     5521 tcagccatca gttttgatat attggccacc gggttgccgg tgctgtggag caatgccgtc
     5581 acctcgccga ggatgctcag tttctcattg atatagctta ctgccttccg gccggtcacg
     5641 gcctctattc ttctgacgcc tgctgcaatg gctccttcgg agacaatctt gaacaatcct
     5701 atccgcgatg tctgggcgac atgtgttccg ccgcacagtt cgactgaatc tccgaatctg
     5761 accaccctta ccctatcgcc gtatttctct ccgaacaggg ctatggcacc cattgcccgg
     5821 gcatcttcca tagggatgtg gtcatgtacc tcggagataa tattctgcat tatcatcgag
     5881 ttgaccttct cttccacagc agatagttct tccggagtca cctggcggaa gtgactgaaa
     5941 tcgaacctca gcctgtcagg ggttaccagg gaacctttct gttccacatg tttgcccagg
     6001 acttccctca gcgcatggtg catgaggtgt gttgccgaat ggttattggc ggtatcctgc
     6061 cgggcttcgg catcaactat tgccctgaat gtcagagacg gatcggctgg cagggcggag
     6121 gcgatatgga tggtcaggtt gttttcctta acagtgtctg taattatgat tttttcactt
     6181 ccggcctcga tgtatccgcg gtcacctgtc tggcctcccg attcagcata gaacggtgtt
     6241 ctgtcaaaca caagctgaac agcctgacgg ccccgtgagg ttactttccg gtaccgtgct
     6301 atccttacct gctcttgggt acggtcatat ccggtgaaga tggtgctttc agtctcacgg
     6361 atcaccaccc agtcgtctgt tgatacggca gcgtcatccc ttgacctttc cttctgggca
     6421 ttgagctcac tcctgaatgc ttcctcgtcg agcgtcatcc tgttttccct gagaatcagc
     6481 tgggtgagat caaccgggaa gccataggta tcaaaaagct caaatgcgtc agcacccggc
     6541 aagatgcttt tcccttcctt ccggagagct tccacccggc gttctatcat cctcagccct
     6601 ttgtcaaggg ttctgaggaa tgactgttcc tcttcgtgga tcacctttga tataatatcc
     6661 tgctgctctt tgagctccgg gtagtgaccg cccatagtct ccaccagtgc aggcaggagc
     6721 ttgtagatga acggcccggt cattccaagg gaggtatagc cgtaccttac ggcacgacga
     6781 agtattcttc tgatgacata accggccctg ttgtttgagg ggagctggcc gtcggccacg
     6841 gagaaagata cagtccgcag gtggtcggca atgaccctgt gtgctatatc ccagtctctc
     6901 ttctctccgt atggtttgcc cgttacttct gcggaggctg ccagcagcgg ctggaagatg
     6961 tctgtgtcat aatttgactg tttgttctga atgaccatgc agagtctctc gaagcccatg
     7021 cccgtatcca cgtgcctgtc aggaaggagt tcaagttcac cgccggcacg gcggttgtac
     7081 tggatgaaca ccaggttcca tatctctatc acccggggat gacccgtgtt cacgagcagg
     7141 tgtccggggg ttgcagccct ctcgctctct gacctgatgt cgacgtgaac ctccgagcag
     7201 gggccgcacg ggcccgtctc acccatctcc cagaaattat ccttttttga tccgtcaagg
     7261 atgtgattct ccgcaccgcc aaagcacttt gcccaccagg ttgcggcctc ttcatcgcgc
     7321 ggtacattct caacgggatc accttcaaat acggtggcgt acagacgatc agcaggaatc
     7381 ccgagccggt cacaaagaaa ctcccatgcc cactctattg cttccttttt aaagtagtca
     7441 ccgaatgacc agttgccaag catctcgaac atagtatggt ggtaggtgtc aagtcccacc
     7501 tcctcaaggt cgttgtgctt gcctgaaaca cgcaggcatt tctgcgagtt tgccactctt
     7561 ttgtattttg caggctggtt gccgaggaaa atgtccttga actgattcat cccggcgttg
     7621 gtaaacatca gcgtggggtc attcttgatg accatcggtg ccgaaggcac tatcctatgt
     7681 tcctttgacc tgaaaaaact gagaaattcc tctcttatct cgttagcctt catattaact
     7741 tcctgtgccg ttatgaaact attttcaagc gggtgcgtaa aattaaagca aattatttat
     7801 gtttgaggaa tagtcgtctt aaaatggagg tttatccaca ctattggtga tgctgaagag
     7861 gaggaattac gtttttgacc acactaacct gcagtttctg cagcacagga gaaacagggg
     7921 agagaggatt ctccggtttg cactctggtt ctctctttcg atagtagtca ctttttttta
     7981 cagcatcatc tttcaccggt tgtttggttc acccaaggag gagatgctca ggcagcaggt
     8041 tgaagagctg aagttgcggt acaccatgct tgaaaagcag attgattatt ccacggcagc
     8101 agtgactgag cttcgttcct ctgacaatac cctgtacagg gcggttcttg acatgcccgc
     8161 catcccggct gatatctctg aaggcggatc gggaggtacc aacaggctgg ctgatctgac
     8221 cggttacctg aattctgatc ttttgatgaa ggtcaggtcg aagtttgatg acctgaaaac
     8281 gcagacaaat attcagtaca actcacttgc agagcttact gcaagagcat ccgagtggaa
     8341 ggagatgtgg gagcatctgc cgtatatcag accggtgaat gtcacactgc ggcttggtga
     8401 tagcatggga tttcgtgaaa agcatccggt gcttggttcg ccacgctggc atttcgggca
     8461 ggatttctgc tgtccgatcg gcaccgaggt gtatgcaacc ggggccggca tcgtggctta
     8521 tgcgggcaac cagcgtgacg ggttcgggat aaaggtggtt attgaccacg gatatggcta
     8581 caggactatc tacgggcacc tcagcgaatt caacgtcagg cagggccaga gggtcacccg
     8641 gggtgacttc ataggacttt ccggtaacac cgggacatca accggccccc atctgcatta
     8701 ccagatagac cttttcggcg cacatgtgaa tcccctctgg tactttcagg acaaccttac
     8761 cgaggaggag tatttcatga tgctcgaata cctcagcaaa ccctgacgga aaaatattaa
     8821 tcaaatttta acgttatcag cagtaacatt tgtatctatg ttttgctatt atttcttatg
     8881 tttgcagggc atagagtatg ccatccgtaa aaatgcccaa aacaaagtat aagtataatc
     8941 atgagacact cagctttgac aggataaagc tgggtctcag acagattttc cttcgctttt
     9001 tcggatattt cgttgcctca ctgctccttg c
//
LOCUS       DAOPBK010000106         8864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7412_length_8864_cov_4.107844, whole genome shotgun sequence.
ACCESSION   DAOPBK010000106 DAOPBK010000000
VERSION     DAOPBK010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7412_length_8864_cov_4.107844"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="PLA17_07265"
     CDS             <1..1684
                     /locus_tag="PLA17_07265"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPK85252.1"
                     /translation="VDLGFAINWDRPVDCMLSWDGTVLSGRRISSGWAEKQYIFFSSG
                     FSKSPASVTMLNAGTVAAGNMTEGRNIACILDFEMEKGEELIVKTGISAVDEAGAAGN
                     LIAEVPGWDFDGHAAAAQQAWQKRLGVIRAASADSSVLKVFYTALYHSMIAPNLYSDA
                     DGRYRGTDREIHNADGFTNYSVFSLWDTYRAAHPLFTLTAPDMVPDFINTMLNIRKQQ
                     GKLPVWSLAGNETNCMIGYHAVPVIADAINKKFQGFDYEEALEAAVESASLDFRGLNH
                     YRNFGYIPSELENESVAKTMEYAIDDWCISQMATDLGKPELADEYLRRSYNYRNLFDT
                     TIMFIRGRMSDGSWRPGYDPLYSHHTRSDFTEGNGWQYTWLAPHDIDGLVSLFGSREM
                     FLQRLDSLFRVTEEVKGEFSSPDISGLIGQYAHGNEPSHHIAYLFSMAGAPEKTESMV
                     RRIMAEMYTDKKDGICGNEDCGQMSAWYVFSAMGFYPVNPADGRFILGSPVLDRATIS
                     LPGEKSFTVIAEGNSPGKTIVSACFLNGEKLDRNYITWEEIRAGGELRFVMR"
     gene            1907..4078
                     /locus_tag="PLA17_07270"
     CDS             1907..4078
                     /locus_tag="PLA17_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPK85253.1"
                     /translation="MKRLILIAVSLSASLILSGQPEFPTGSPLFPVIENWHEISVDPQ
                     HAEAMRAVDQPKDQPFRFAIPVEVSLTPSNSGFIATRGSETIWVLPLSSKGALSLNII
                     LSPYNLPRGAYIYVYDHDRQTIRGAFTSGSTSATLSMPVMPVPGERLVLECHFPGTRI
                     PENSVGVTQVAHDFIGFFGPEGIKDYYYGRSGECEIDISCSTNPLYLTASRSVVRLLV
                     AGTELCTGVMVNNTGNLNKPYLITANHCIEKKSHATNTIFVLNYRSPSCAGPDMTNMY
                     TLTGSQLRATNPDIDFTLVELNQFPSLIARPYFAGWDITTTTPTNTFTIHHPEGDVMK
                     LSIDDNAPVTASYPEEGFISSGFWRVLRWDLGTTERGSSGGPLFDQNGRLRGTLTGGA
                     ATCNNPANDYYAKLIRMFDITSIDTSHLKPWLDPAGTGATLVSGRDPYEYNLSRSDTI
                     GGIPANDPGTTDTYSSPGWGLATGNNSDGLTRYAEYIPFSGTGEIAWLRLQVSASSFI
                     NAADSVRFYIWDGGALPGNVIASRKLRINETRGGTVLEVDFGRTIKVTRSFYAGYAVY
                     YRNPIDQPQPQFAVARSAPWALPSQNTAYFNTGSAWEPFTRHPSFPMAVSLGIKAVMV
                     ENTIFNQPEDPEQETTDLKVYPNPFSSEVTFILAGRGVTSTTITIYDNSGRVVSAGEY
                     RNIFPGELTVPLPGLAPGIYHYGLRADSVYYSGTLVKAGMN"
     gene            complement(4144..5574)
                     /locus_tag="PLA17_07275"
     CDS             complement(4144..5574)
                     /locus_tag="PLA17_07275"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HPK85254.1"
                     /translation="MMKFRLSTLILPLAFVTLTTIARSQAGHSITVTVRDAPSVKLRL
                     AYHVGNQQYLRDSLVTDSGGRGKFTGSERLPAGVYMIVLPDNHFFEFLIDEEQFFDIR
                     FSKSDPVATVSFSGSEENTGFVAYQRRWKSLQEEAMEISGRLGGLQPGSPETSAARKE
                     LTDQEKKMKQYLNEVAETNKGTLLGAIARSVIPVETPAATVPPGTHNPDSVTRLLSYL
                     YFKDHFFDHIDFSEPGLIRSPVLGGKLEQFFSQVVVQMPDSINKEADRLLDMSAVDSS
                     MFQYVTVWLMNRYASSEIMGHDAVVVHLADRVYLAGKAPWAADEYISDLRKRIERLRT
                     NLIGMKAPELLMGSFAGHYVSLYDVKAEFTILYFWEPDCGHCKESTPLLKEYYEKNKE
                     SGIVVFAVCTHHDREKWERYIADNDLTWINGWDPERMSRFDFLYNVESTPLVYILDRD
                     KKIIAKRLSVNDIPAFIDTWRKFQSH"
     gene            complement(5713..6414)
                     /locus_tag="PLA17_07280"
     CDS             complement(5713..6414)
                     /locus_tag="PLA17_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPK85255.1"
                     /translation="MEITIKEFIKGAQEAQGVTVIIDVFRAFSTACYAFDSGVNRLIN
                     VATPEEAFALRDKLKAEVIIAGEQGEKKPAGFDYGNSPTEMLTGSVAGKTMIHCTTAG
                     TRGIISAAGATEILGAGLVNAGAVASYISMIRPEKVTLVAMGYRARETAEEDLLCARY
                     IRELLEGCESDISKEMEALREGSGSRFFNPANLAFSPPTDFFLCTDLNRFNFALRAVI
                     TAGGYAEILRINMDH"
     gene            6500..7834
                     /locus_tag="PLA17_07285"
     CDS             6500..7834
                     /locus_tag="PLA17_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPK85256.1"
                     /translation="MRPVGEIRQLIVSRAGQLWRSITGRLKKSAPFWVRQRRIVYLLF
                     KGIKRENIYIKASALTFFTILSAIPIVALAFGIAKGFGLSDELRAQIIMQFHNQEQVM
                     NWILDFANKTLEQTSGGWLAGIGVVFLFSAVVQLLKYVENTFNSIWKVDETRVWYRQV
                     TDYLAVIILVPALFIASSSVTVLATTRLTAMLSQSDMLEGLKPVVSFLVQLIPFILLC
                     SLSTGAFLAMPNTRVRFRSALIAGLAAGIALQVLQIVYVETQMGITRLGTLYGSFAAI
                     PLLMIWVQMSWVVLLMGAQLSYYLQNITRYEFEFDVQTVSPRQKKRLLLLVMHSLIVD
                     FVKGAKPRAPEEISLELNLPVRTVHECLDQLHEASMVTEVWNDEQEKYVYQPATDINK
                     MTLSFVLEKLESSGSLHKTVINNSDYRKIDTALTRFESLIASSETNILLRDL"
     gene            complement(7836..8537)
                     /locus_tag="PLA17_07290"
     CDS             complement(7836..8537)
                     /locus_tag="PLA17_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HPK85257.1"
                     /translation="MKTFLRRFENTRDLVILFGTWGIDDKAFSNLCTDNHDFILFYNY
                     SADEPLILPEMKTYRRVTVIGWSLGVWAAEYYSRKLGIKPDLTIAINGTPVAADDKYG
                     IPLKIFEATLNNISNYSMARFYLSLFGTRSLFEVNRAYIPTRTEKSLHDELRWLYNRM
                     MEPVETGFVWDYALIGKEDSIFKASNLKSYWSMHPETRQIMVDAPHYLFGYWNSLEEM
                     IRYVRRFRLSPFRNK"
BASE COUNT         2153 a         2234 c         2201 g         2276 t
ORIGIN      
        1 tgtcgatctg ggttttgcca tcaactggga ccgccccgtc gattgcatgc tgtcatggga
       61 cggcaccgtt ctcagcggca ggcgcatctc ctcaggctgg gctgaaaaac agtatatctt
      121 cttctcttca ggtttttcaa agtcgcccgc ctcggtcact atgctcaatg ccgggacggt
      181 tgcggcgggt aacatgacag agggcaggaa tattgcatgc atccttgact ttgagatgga
      241 gaagggtgag gagcttattg ttaaaacagg aatttctgcc gttgacgagg cgggcgcagc
      301 gggtaacctt atcgccgagg ttcccggatg ggacttcgac gggcacgctg ctgccgcaca
      361 gcaggcatgg cagaagaggc tcggcgttat cagagccgca tctgctgaca gctcagtgct
      421 gaaggtcttc tacaccgccc tctatcattc gatgattgca ccaaatctct attccgatgc
      481 cgacggcagg taccggggga cagacaggga gatacacaat gcggacggat tcaccaatta
      541 ttcggtcttt tcgctgtggg atacctatcg tgccgcacac ccgctgttca ccctcacagc
      601 tccggatatg gtgcctgact ttataaatac catgctcaat atcaggaagc agcagggcaa
      661 gctgcctgtc tggtcactgg ccggaaatga gaccaactgc atgatcgggt accatgcagt
      721 gcctgtcatt gccgatgcca taaacaagaa atttcaggga tttgactacg aggaggcgct
      781 tgaggctgca gtggagtcag catccctcga tttcaggggt ctcaaccact accgtaactt
      841 cgggtatatt ccttctgaac ttgaaaatga gtcggttgca aagacgatgg aatatgccat
      901 cgatgactgg tgcatctcac agatggccac cgacctcggg aaacctgaat tggcagatga
      961 gtatctgagg cggtcataca actaccggaa cctgttcgat acaacaatca tgttcatcag
     1021 aggccggatg agtgacggca gctggaggcc cggctacgat ccgctctatt cgcatcacac
     1081 cagatccgac ttcaccgagg gaaacggctg gcagtacaca tggctggcac ctcatgacat
     1141 tgacggcctt gtatcactct tcggaagcag ggagatgttc cttcagcggc ttgattctct
     1201 cttcagggtg acggaggagg ttaagggcga gttctcatcg cctgacataa gcgggcttat
     1261 aggccagtat gcccacggca atgagccatc acaccatatt gcctacctgt tttccatggc
     1321 cggagcgccg gagaagaccg aatcaatggt gcgtcgcatc atggcggaga tgtatactga
     1381 caaaaaggat ggtatatgcg gaaacgagga ttgcggccag atgtcggcat ggtatgtctt
     1441 ctcagccatg ggattctatc ctgtcaatcc cgctgacggc aggtttattc tcggaagccc
     1501 ggtccttgac agggccacca tttcgcttcc cggggaaaag agcttcactg tcatcgccga
     1561 aggcaattcg cccggtaaga caattgtttc cgcctgtttt ctgaacggtg aaaagcttga
     1621 ccgtaattac atcacctggg aggagataag agcaggtggt gaactcaggt tcgtgatgag
     1681 atgaacaacc ctcaggagaa aggcttttcc cggacttaat gcggaccacc gcaggaatac
     1741 tggcagccac tgatacaatt gattatcagc agaaaggaat cctgcttagc tgcagcttca
     1801 ggaaaaagct taatgatacc cgacaggcag gtgtaattcc cggttttatg cattatatga
     1861 aggattagct tctttttgct atttttagag ccttaatcgg aagcaattga agagactaat
     1921 actgatagcc gtaagcctct ccgcatcgct tatcctgtcc gggcagccgg agttccccac
     1981 aggaagccca cttttcccgg tgatagaaaa ctggcatgag atcagtgttg atccgcagca
     2041 tgcagaggca atgcgcgcag tggatcagcc aaaggatcag ccattccggt ttgcaatacc
     2101 ggttgaagtc agcctcaccc cgtccaattc cggattcatt gccacgagag gatcagaaac
     2161 catatgggta ttgccactct catcaaaggg cgctctttct ctcaatatta tcctttcacc
     2221 ctataacctt ccccggggag cctatatcta tgtttacgat cacgacaggc agacaataag
     2281 gggagcattc accagcggga gcacctccgc taccctttcc atgccggtta tgcctgttcc
     2341 gggggagaga ctggtactgg aatgtcactt cccgggcacc cgcatacctg agaactcagt
     2401 gggtgtgacg caggtggcgc atgatttcat tggttttttc ggcccggaag ggattaagga
     2461 ttattattac ggacgctcag gcgaatgcga gattgatatc agttgttcga ccaatcccct
     2521 ctatcttact gcttcccgct ctgtggttcg tctcctggtg gccgggaccg aactctgtac
     2581 cggtgttatg gtaaacaata cagggaacct gaacaagccc tacctgatta ctgcaaatca
     2641 ttgcatagag aagaagagtc atgcaacaaa tacaatattt gttctcaatt accggagtcc
     2701 atcctgcgcc ggaccggaca tgaccaacat gtataccctg accggttctc agctcagggc
     2761 taccaatcct gacatagact tcaccctggt ggaactgaat cagtttccct cgctgattgc
     2821 caggccttac tttgcaggct gggacataac aactacaact cccacaaaca cctttaccat
     2881 ccatcacccc gagggtgatg tgatgaagct ttcaattgac gataacgccc ctgtcacggc
     2941 atcctatcct gaagagggat ttatctcgtc aggattctgg cgtgtgctca ggtgggatct
     3001 tgggacaact gagagaggat cgtcaggagg gccgctgttt gatcagaatg gcaggctcag
     3061 gggaacactt accggaggag cggctacatg caataatccg gcaaatgact attatgccaa
     3121 gcttataagg atgtttgaca taacatccat cgacaccagt catctgaagc catggcttga
     3181 tccggcaggc acgggagcca ccctggtcag tggccgtgat ccctatgagt ataacctctc
     3241 caggtcagat accattggag gaattccggc gaatgacccc ggcacaacag acacatactc
     3301 atccccggga tggggccttg ccaccggaaa caactccgat ggcctgacca gatatgcaga
     3361 gtatattcct ttctcaggca ctggtgagat agcctggctc aggctacagg tctccgcttc
     3421 atccttcatc aatgccgctg actcggttcg gttttatatc tgggacggag gcgctctgcc
     3481 gggaaatgtt atagcctcac gaaaactccg gattaacgaa accaggggtg gtacagtgct
     3541 ggaggttgat ttcggaagga ctataaaagt tacacgatcc ttttatgccg gctacgctgt
     3601 ctattacagg aatccgatag accagcccca gcctcagttt gccgtggcac ggtcggcacc
     3661 atgggccttg ccctcacaga atacagctta tttcaatacc ggatcagcat gggagccatt
     3721 cacccggcat ccttcgttcc cgatggctgt ttcgctcggg atcaaggctg taatggttga
     3781 aaacaccata ttcaaccagc ctgaagaccc tgaacaggag actacagacc tgaaggttta
     3841 tcctaatcct ttctcctcag aggtgacttt catacttgcc ggaaggggag tgaccagcac
     3901 aaccataacg atatatgaca atagcgggag ggttgtatct gcaggggagt acaggaatat
     3961 cttcccgggc gagctcacag taccattgcc ggggctggca cccgggattt accactacgg
     4021 tctgagggct gactcggtct attattcagg tacgctcgta aaagccggta tgaattgacc
     4081 aggtttcccc tgccgggtca tgatcagtga ggccggaact tcctcaaggt atgaaggctc
     4141 ttatcagtga gactgaaact tcctccaggt atcgataaat gccgggatgt cattcaccga
     4201 gagccgcttt gctatgattt tcttgtcccg gtcgaggata tagaccagcg gtgtggattc
     4261 aacattgtaa aggaagtcga acctgctcat ccgctcagga tcccatccgt tgatccatgt
     4321 caggtcgtta tctgctatat acctctccca tttttcacgg tcatgatggg tgcagaccgc
     4381 aaataccaca attcccgact ctttgttctt ctcgtaatac tctttcaaca gcggggttga
     4441 ctccttgcaa tgcccgcaat caggttccca gaaatacaat attgtgaatt cggccttcac
     4501 atcgtacaat gagacataat gaccggcaaa tgatcccata aggagttccg gggccttcat
     4561 tcctataagg ttggtcctca gacgctcaat tcttttcctc aggtctgaaa tatattcgtc
     4621 cgcagcccat ggcgccttgc ctgcaagata gaccctgtca gccagatgca ccacaacggc
     4681 gtcatgaccc atgatctcgc ttgaagcata cctgttcatc agccagacag tcacatactg
     4741 gaacattgaa ctgtcaacag cgctcatatc aagaagcctg tcggcttcct tattgattga
     4801 gtcgggcatc tgtaccacca cctgactgaa aaactgttca agttttcctc caagaacggg
     4861 actccggatc aggccgggct cggaaaaatc aatgtggtca aaaaagtgat ccttgaaata
     4921 aagataggag agaaggcgtg tcacggagtc cggattatgc gtgccgggag gcactgttgc
     4981 ggcaggtgtc tcaacaggta tcactgaccg tgctattgct ccgaggagcg tacccttgtt
     5041 tgtttcagca acttcattga gatattgttt catcttcttc tcctggtccg tgagctcttt
     5101 tctggcggct gatgtctccg ggcttcctgg ctgaagcccg ccaagcctgc cgcttatttc
     5161 cattgcctcc tcctgaagcg atttccatct cctctggtag gccacaaaac cggtgttctc
     5221 ctcagaacca ctgaaagaaa cggtagcaac cggatcgctc ttgctgaagc ggatatcaaa
     5281 aaactgctcc tcgtcaatca ggaattcaaa aaagtgattg tcaggcaata caatcatata
     5341 gacccctgca ggcaacctct cacttcccgt gaactttccc ctgccgccgg agtccgttac
     5401 aaggctgtca cggagatact gctggttgcc aacgtgatat gcaagcctta gtttgactga
     5461 tggagcgtcc cggactgtca ctgtaataga gtggccggcc tggcttctgg caatggttgt
     5521 cagtgttaca aaagccaggg gtagtataag ggtagaaagt ctgaatttca tcataatagt
     5581 atccggtgta caattatcct gccatttatt gtgaaggtgg cactcatcac atcaacgctg
     5641 aggcctttgt ttttcttatg tttctcagtc acagggcggt tgttttcagt tttccgcttt
     5701 cacgctcaaa tctcagtgat ccatgttaat cctgagaatc tcggcgtatc cgccggcagt
     5761 gattacggcc ctgagcgcaa agttgaaacg attcaggtca gtgcagagga agaagtctgt
     5821 aggagggctg aaagcaagat tggccgggtt gaagaacctg cttcccgatc cttcccgcag
     5881 tgcctccatc tcctttgata tgtcgctttc acaaccttca agcagctccc tgatgtacct
     5941 tgcgcagagc aggtcttcct ccgctgtttc ccgggccctg tagcccatgg caaccagggt
     6001 taccttctca ggcctgatca ttgagatgta tgatgcaaca gcccctgcat ttaccagtcc
     6061 tgccccaagg atttccgttg ccccggcagc gctgataatg cccctggttc ctgccgtggt
     6121 acagtgtatc atcgtcttgc cggcaactga acctgtcagc atctcggtcg gactgttccc
     6181 gtagtcgaat ccggcaggtt tcttttcacc ctgttcacct gcaataatta cctcagcctt
     6241 tagtttatcc cttagtgcga atgcctcttc aggcgtggca acgttgataa gacggtttac
     6301 accactgtca aaggcatagc atgcggtgct gaaggcacgg aagacatcaa tgataactgt
     6361 cactccctga gcctcctgcg cccctttaat gaattccttt atggtgattt ccatgtcggt
     6421 tttttcaatt tgtgaagata ataatagtgt ttgaagatca ttttataaat ttgcattccc
     6481 tcaataatcc gaaaatccgg tgagacctgt tggtgaaata cgtcagttga ttgtcagccg
     6541 tgccggacag ctctggcggt caattaccgg gaggctgaag aagtcagcac ccttctgggt
     6601 tcgccagagg cgtattgtat acttgctttt caagggaata aagagggaaa acatttacat
     6661 caaggcctct gcactgacct ttttcacgat tttgtcagcg atacccatag ttgcgctggc
     6721 atttggaata gcaaagggat tcgggctgtc agatgaactc agggcacaga ttataatgca
     6781 gttccataac caggaacagg ttatgaactg gattcttgat tttgcaaaca agacacttga
     6841 gcagacaagc ggaggctggc tggcagggat aggagttgtc tttctctttt cagcggtggt
     6901 acagttgctg aaatatgtcg agaacacttt caattccatc tggaaagttg acgaaacccg
     6961 ggtatggtat cggcaggtga cagattatct tgcagttatc attcttgtgc cggcactttt
     7021 cattgcttca agcagtgtca cggtccttgc taccacgcgc ctgactgcca tgcttagcca
     7081 gtcagacatg ctcgaggggc tgaagcctgt tgtctccttc ctggttcagt tgataccgtt
     7141 catattgctc tgctctcttt ccactggtgc cttccttgcc atgcccaaca ccagggtcag
     7201 gttccgttcg gcgcttattg ccggacttgc cgcaggtatt gcgttgcagg ttttgcagat
     7261 agtttatgtc gaaacgcaga tgggcatcac caggcttggc actctttacg gaagcttcgc
     7321 tgccattccg ctgctgatga tctgggtaca gatgagctgg gtagtgcttc tgatgggagc
     7381 ccagctatca tattaccttc agaatatcac ccgctacgaa tttgagtttg atgttcagac
     7441 agtttcgccg cgtcagaaaa agaggttgtt gttgctggta atgcattcac tgatcgttga
     7501 ttttgtgaaa ggggccaaac cgagggctcc cgaggagatc tcgctggagt tgaacctgcc
     7561 tgtgcgcact gtccatgaat gccttgacca gcttcatgag gcttccatgg tcaccgaggt
     7621 ctggaacgat gagcaggaaa aatatgttta ccagccggcc actgacatta ataaaatgac
     7681 cctctcgttc gtgcttgaga agcttgagtc atcgggctca ctgcataaaa cagtgataaa
     7741 caattccgat taccggaaga tagacactgc cctgaccagg ttcgagagcc tgattgcctc
     7801 gtcggaaaca aatatccttc tgagggatct gtaacctact tgttccggaa cggtgagagc
     7861 ctgaagcgtc tcacatacct tatcatttcc tcgagtgaat tccagtaacc gaagagatag
     7921 tgcggtgcat caaccattat ctgccttgtc tcagggtgca ttgaccagta gcttttaagg
     7981 ttcgaagcct tgaagatact atcctcctta ccgataaggg cataatccca gacaaatcct
     8041 gtttcaaccg gttccatcat ccggttgtag agccatctca gctcatcgtg gagcgatttt
     8101 tccgtacggg ttggtatgta ggcgcggttc acctcaaaca ggcttcgtgt gccgaagaga
     8161 ctgagataaa acctggccat actgtagttg gaaatattgt tgagggtagc ttcgaaaatc
     8221 ttcagcggta tgccatactt gtcatctgct gccacggggg tgccgttgat ggctattgtc
     8281 aggtctggct ttattccaag tttcctgctg taatattctg cagcccagac gcccagtgac
     8341 cagccgataa ctgtaacacg cctgtaagtt ttcatttccg gcagaatcag cggctcatct
     8401 gccgaatagt tgtagaacag gatgaaatca tggttgtcag tacagaggtt ggagaaggct
     8461 ttgtcatcta ttccccaggt gccgaagaga ataacaagat ccctggtgtt ttcaaatctg
     8521 cggaggaatg ttttcatcgg ataggggaaa ataaaaaaga ggagagggtt acagtggtac
     8581 tttaaggaac gggcttatga gataatcccg gaatctactg tctgataaaa ggaagtaacc
     8641 tatggtagtc agcctatagg tccatgtgaa agtcaatgtc tgcaatacaa tcctattgat
     8701 tcccacaggt aacccttact ctcctccttt atttcatcat ctgtacaaat gtaatacata
     8761 aacctcaact ttgcaagcaa tctcagaaat aatttcaggt actatatgtg tgacaggcta
     8821 gtatatcatg acaatttttt tatcaaagat aattatacgg acgg
//
LOCUS       DAOPBK010000107         8822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7451_length_8822_cov_5.487738, whole genome shotgun sequence.
ACCESSION   DAOPBK010000107 DAOPBK010000000
VERSION     DAOPBK010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7451_length_8822_cov_5.487738"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1735
                     /locus_tag="PLA17_07295"
     CDS             <1..>1735
                     /locus_tag="PLA17_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK85258.1"
                     /translation="GNFIAGNVTSLTYSYSNTFREALNNNYSIYNYAYDTPGILDEFT
                     DRQYKTNVRAGLMHNWTLYPYDGLKLEFRNFFTSTSGSAVSMREGLDWYNNGRSIRSG
                     ETKYTHRVIYSGQLGAEHLFAEGRTTVDFTAGYSISARKEPDIRRYRYISNDGDISPY
                     VILFGSNPDLSSVSRMWLDLDETSFSGMLNLIQKFGKGINNWEIRTGIFAENRDRSFR
                     ARNFGYAISSDTSPFASTSLPVQEIFAPENINLTDGIRLKEITSLSDSYEASMMVLAG
                     YLNFRFRFGTRTDLSVGVRIEKERQELSSYRQGTSIPVNVVRDTVNLFPSGNLAINLN
                     SSSLIRLTYGLSVNRPEFREIAPFYFVDFEQNAGIYGNEQIKSAYIHNLDLRYELYPG
                     NNETFNAGIFYKHFTNPIEVSIRGNNPTQFSFDNISSAYSFGLETEARKNLGFISDRL
                     EKLTAVVNLSLIRSSVAFDRPLQGQSPYIVNVGLFWQDVDRGLMASVLYNRIGKRIIA
                     VGRPSPNSWESIPDIYEMPRNETDLAISKKIGKMLEIKAGIKDLFGEKVVFQQHVNSI
                     VDMTNYGGDGMK"
     assembly_gap    1736..1745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1832..3463
                     /locus_tag="PLA17_07300"
     CDS             1832..3463
                     /locus_tag="PLA17_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPK85259.1"
                     /translation="MKTNIFSVFSRVMALGLFLTVMVSCEKEIPVTSLTLDKAEATVQ
                     VGANLTLLPVIDPLNATNKEVQWTTSNASVATVTDGVVTGVALGSAVITAASVENPLF
                     KAECNITVVPSTGQVITVSGDITTDTRWYAQAKYHLSGFVYVKNNATLTIEAGTIIKG
                     VSNTKATLIIERGSKIIAAGTATQPIVFTSDKPAGQRASGDWGGVVICGKAKTNKHDD
                     GEGVGIAEGGIGSKYGGSDDNDNSGILQFVRIEFPGIPLTSTANSEINGLTLYSVGKA
                     TVIDHIQVSYSGDDSFEWFGGNVNAKYLVALGGLDDAFDTDNGFSGKVQFGLILQDPL
                     KSDQSGSNGFESDNDADGSLLTPVTSPIFTNVTAIGPLAVTATLPEGTKHQKALHLRR
                     GTMTSIYNSVFVGLPQGLSIDGQKGNSPTRADANELQIENTILAGMTDLYVEKTGTVA
                     VPYTVAQHEAYFRAEARNNRDDMTMEEVIGTGFISLTSPSLLPKAGSPLLSGASFTNA
                     RLTDSFFTVTSYRGAFGTENWTSGWCNWDPQNTVY"
     gene            complement(3686..4033)
                     /gene="rplS"
                     /locus_tag="PLA17_07305"
     CDS             complement(3686..4033)
                     /gene="rplS"
                     /locus_tag="PLA17_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPK85260.1"
                     /translation="MDLMKVVEKEFASGVELPKFRAGDTVTVHYKIREGNKERIQQYR
                     GVVIQRSGAGMTATFTVRKMSGNIGVERIFPVASPFIDKVEVNKHGKVRRARIFYLRN
                     LTGKKARIKERIF"
     gene            complement(4137..5852)
                     /locus_tag="PLA17_07310"
     CDS             complement(4137..5852)
                     /locus_tag="PLA17_07310"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPK85261.1"
                     /translation="MKKTTSLMLSLILMMFIISEKSHACTNFLITKGASKDGSTMITY
                     SADSHVLYGELYFWPAMKYKPGTMFDVYEWDSGRFLGTIEQAPVTYSVVGNMNQYQLA
                     ISETTYGGRGELRNRDGLIDYGSLIYLTLQRAKNAREAIHTIVTLTEKYGYCSSGESF
                     SISDPNEVWIMEMIGKGQGKKGMAFVARLVPDGYISGHANQARIQTFPQANGTTSITS
                     AEIDKIFNPSVETVYAADVVTVARDFGWYDGTDADFSFSDVYAPVDFGAARFCDARVW
                     AGFNRVNSTMGEYLNYAMGYDLENRMPLWVKPDNKLSLEDVMGLMRDYYQNTPMDMTK
                     DIGAGPYANTVRWRPMSWKVDGKEYLHERATSTQQTGFSFVTQSRSWLPDPVGGILWF
                     GVDDTYYTVYTPMYCGITSVPHSFAVGNGDIMTYSADAAFWVFNEVTNFVYSRANVMT
                     PDLQAKQKELEHKYMAETADIDARAAELFNSTAKGAAKKGTDMITAYSVNAGNNTVKE
                     WRELYKFLFTKYMDGNVKTRRPVPEGYKYIAPEVKQPGYPQEWLMRIVIDAGDKLKVP
                     ESAGN"
     gene            complement(5949..6692)
                     /locus_tag="PLA17_07315"
     CDS             complement(5949..6692)
                     /locus_tag="PLA17_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HPK85262.1"
                     /translation="MVEICADSVESATVAEAAGAGRIELCSALAEGGVTPSAGLIEAV
                     RRNTGIKIHVLIRPRGGDFLYSDSEFSVMRRDIEVAGENGADGIVTGILNRDGTIDVE
                     RTALLAEYAAPMSLTFHRAFDLCRDAKKGIEDLIAAGAERILTSGQAKSAIDGTTLIK
                     SLISWSAGRIIIMPGGGIDEYNIGLLAAGTGAREYHLSGRRQRESMMTFRRKGIYMGD
                     PRLQSEYILKSADAERISSVIMILKGIVF"
     gene            6772..7671
                     /locus_tag="PLA17_07320"
     CDS             6772..7671
                     /locus_tag="PLA17_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPK85263.1"
                     /translation="MKPTLMVLAAGMGSRYGGNKQLDQVGPSGETIIDYSIYDARRAG
                     FGKVVFVIRRDIEEQFRETFVKRLRDVIEIDYVFQDLGNLPGGYKVPEGRQKPWGTSH
                     AIMVTEPKIHEPFGVINADDYYGVDAFKVLYSFLTEDTNDRNYCIVGYRLGNTLSDHG
                     HVNRGICRVSSDGFLEHIVETRGIEKRPDGIFAPDAEGNVNRYTGDEIVSMNLFGFKP
                     SVYKYLNMEFTNFLKERMSDPKAELDIPTSLDKFVKKGEITIQILRSNSQWFGVTYRE
                     DKPFVVESIRKMVDQGMYPEKLF"
     gene            complement(7743..8540)
                     /locus_tag="PLA17_07325"
     CDS             complement(7743..8540)
                     /locus_tag="PLA17_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85264.1"
                     /translation="MMKKKQIYIVSLGLALLLFSCEYYIGLNQQPKFVNKHTEEGLNV
                     FGLLRPDSTEGFNKSFVYVNRVFPALELEGYDILKPAFVKIDKIVQDSLTESVVFSLL
                     PSQTVRTDTVYRPATYFSPGAGERYRLTCKYEGMPDAIGETIIPSKPQIDLETLITDR
                     KMISFSLLPDSLTGMVDIYQIADNDNHLLVSRLVTFPDRKTDVEIILPFDHSGTRLMI
                     CAYDQNMAAYIGNSNISLNFNKFRTTISTLMSGFGVFGSMNFYEMEL"
     gene            complement(8638..>8822)
                     /locus_tag="PLA17_07330"
     CDS             complement(8638..>8822)
                     /locus_tag="PLA17_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85265.1"
                     /translation="FSELIHAKESYMIVTLRNLTFFRRNVEYFFPLYVGSDDSRYLPI
                     GTNYLPSLGFSYSIKF"
BASE COUNT         2366 a         2038 c         2046 g         2362 t
ORIGIN      
        1 ggaaacttca ttgccggtaa tgtcacatca ctgacctata gctattcaaa caccttcagg
       61 gaggcgttga acaataatta ctcaatatat aattatgcgt atgacactcc gggaatactg
      121 gatgaattca cagacaggca gtataaaaca aatgtccggg caggcctgat gcataactgg
      181 actctctatc catacgatgg attaaagctg gagttcagaa actttttcac cagcacttcc
      241 ggatcagccg tttctatgag agaaggcctt gactggtaca acaatggacg ctcaatacgt
      301 tccggtgaga caaaatatac gcacagggtt atctattcag gacagcttgg cgctgaacat
      361 ctcttcgcag aagggagaac tacggttgat ttcacggcag ggtattccat atcagccagg
      421 aaagagccgg acataaggcg gtaccgttat ataagcaatg atggagatat ctcgccatac
      481 gtcattctct tcggaagcaa ccctgacctt tcatctgtat caaggatgtg gctggatctt
      541 gatgaaacaa gcttttcggg aatgctcaac ctgatacaaa agtttggtaa ggggattaat
      601 aactgggaga tcagaactgg gatttttgca gagaaccgtg acaggagctt cagggcacgc
      661 aatttcgggt atgctatatc atctgacacc tcgccatttg cttccacatc tctccctgta
      721 caggagattt ttgcacctga aaacatcaat cttacagatg gtatcaggtt gaaggagatc
      781 acctcccttt ccgattcata tgaagcatcg atgatggtgc tggccgggta tctcaatttc
      841 aggttcaggt tcggtacacg gaccgatttg tcagttggtg tgaggatcga aaaggagaga
      901 caggagctgt caagctacag acagggaacc agtataccgg tcaatgttgt acgtgataca
      961 gtcaacctct ttccttcagg caatctcgcc ataaatctca actcatcgag cctgataaga
     1021 ctgacctacg gtttgtcagt caaccggccg gaattcaggg agatagctcc gttctacttc
     1081 gttgatttcg agcagaatgc aggaatttac ggtaatgagc agattaaatc agcctatatt
     1141 cacaatctgg atcttcgtta tgaactatac cctggcaaca acgaaacatt caatgcaggt
     1201 atattttaca agcacttcac caatcccatt gaggtctcaa taaggggcaa caatcccacc
     1261 cagttctcat ttgataatat cagctctgca tacagcttcg ggctggagac agaggctcgc
     1321 aagaacctgg ggttcatctc tgacaggctt gagaaactta ctgccgtggt caatctttcg
     1381 ctgatcagga gctccgtggc ctttgacagg ccactgcagg ggcagtctcc ctatattgtc
     1441 aatgtcgggc ttttctggca ggatgttgac aggggattga tggccagtgt gttatataac
     1501 cgcataggta aaaggattat agctgtcgga aggccttcgc ccaactcctg ggagagcata
     1561 cctgacattt acgagatgcc gcgaaatgag accgacctgg caataagcaa aaaaataggc
     1621 aaaatgcttg agataaaagc cgggatcaag gacctgttcg gtgagaaggt ggtttttcag
     1681 cagcatgtaa atagtattgt tgatatgacc aactacggtg gtgacggcat gaaggnnnnn
     1741 nnnnnaccag gtcgtggtac ccgggcagac agtattcgct tggaatatca cttaaatttt
     1801 aagaaaacaa ccatttaaca aaacacaaat catgaaaacg aacatttttt cagttttttc
     1861 cagggtgatg gcacttggat tatttctgac cgttatggtg tcatgcgaga aagaaatacc
     1921 ggttacttcc ctcactcttg acaaggcgga ggccacagta caggttgggg caaatctgac
     1981 actgcttcct gttattgatc cgctgaatgc cacaaacaag gaggttcaat ggacaaccag
     2041 caacgccagt gtagcgacag tgactgacgg agtggtgact ggtgtggcac tgggaagtgc
     2101 agtaataacc gctgcttcgg ttgaaaatcc tctgttcaag gcagaatgca atattacagt
     2161 tgtaccatct accgggcagg tcataaccgt ctcaggagat ataaccactg ataccaggtg
     2221 gtacgcacag gcaaaatacc acctttcagg attcgtctat gtcaaaaaca atgcaaccct
     2281 tacaatagaa gcaggtacaa ttataaaggg ggtatcaaac accaaggcaa cgctcatcat
     2341 tgagagaggt tcaaagataa ttgctgcagg cacagccacc cagccgatag tttttacatc
     2401 tgacaagccg gcagggcaga gggcaagcgg agactggggc ggagttgtta tatgcggaaa
     2461 ggcaaaaacc aacaagcatg atgacggtga aggtgttggc atcgctgaag gtggcatcgg
     2521 ttccaagtac ggtggcagtg atgacaacga caattcaggt attcttcagt ttgtcaggat
     2581 tgagttcccc ggaattcccc tcacctccac tgccaacagt gagatcaacg gactgacact
     2641 ctactctgtc gggaaagcca ctgttattga ccacattcag gtttcatatt caggtgacga
     2701 ctccttcgaa tggtttggtg gcaatgtaaa cgcaaaatac ctcgttgctt tggggggact
     2761 tgatgatgca tttgatactg ataacggctt cagcggtaag gttcagtttg gtcttattct
     2821 gcaggatcct ttgaaatcag accagtcagg ttcaaatggt ttcgagagcg acaatgatgc
     2881 agacggttcg cttctcacac ctgttacctc tcccatattc accaatgtta ccgccatcgg
     2941 acctcttgca gtaacagcca ctctccctga agggacgaaa catcaaaagg cgctgcatct
     3001 tcgcaggggc acgatgacct cgatatacaa ctcagtgttt gtaggactcc ctcaaggact
     3061 gtcgatagac ggtcagaagg gcaactcgcc aacacgtgct gatgcgaatg agttgcagat
     3121 tgagaatacc atacttgcag gaatgacaga cctctatgtt gagaagaccg gtactgtagc
     3181 cgtaccctat acagttgctc agcacgaggc atattttaga gctgaagcaa gaaacaacag
     3241 agacgatatg accatggaag aagtcattgg caccggtttc ataagcctta cctctccgtc
     3301 tctgcttcca aaggccggat caccgctcct tagcggagcc tccttcacca atgcaaggct
     3361 tacagacagc ttctttaccg taacatccta caggggtgct ttcggaacgg aaaactggac
     3421 ttccggatgg tgcaactggg acccacaaaa cacggtgtac tagtccagaa attgatggtt
     3481 tgacaacctt ttaccagggg gcacaccgga gtagccccct ttttttgttg caggaataaa
     3541 agagccgccc ggtggaggcg gctcttccat gttgtgagag acaggtaaac aaagcaggag
     3601 gagccctgtt gcgttttcct cctgcctgat tgcatgaaaa agccgcccgt tgaggcggct
     3661 ctttcatgta tctgtctgat gagtatcaga atattctttc cttgatgcgt gccttctttc
     3721 cggtcaggtt cctgaggtag aagatgcgcg ccctgcgtac cttgccgtgc ttgttaacct
     3781 caaccttgtc aataaatggc gatgcaaccg ggaaaatcct ttctaccccg atgtttcctg
     3841 acatcttgcg gacagtgaat gtagctgtca tccctgctcc tgatctctgg attacaacac
     3901 cacgatactg ctgtatcctc tccttgttgc cttccctgat cttatagtgt accgttacag
     3961 tgtcacctgc cctgaatttc ggtaactcaa ccccggaggc aaactccttc tctactactt
     4021 tcattaagtc catcgcgcaa atatgtttat gtgaccttta ctctttttaa ataagtcgca
     4081 aatgtagcaa tattttttta ttatctgcaa aatatttatt gcaattacag taaatatcag
     4141 ttacctgcgc tttcaggcac tttgagtttg tcgccggcat cgattacgat tctcatcagc
     4201 cactcctgcg ggtagcctgg ctgtttaacc tcgggggcga tatacttata gccttccggg
     4261 acaggacggc gggttttaac gttgccatcc atatatttgg taaagaggaa tttgtatagt
     4321 tctctccact cctttaccgt gttgttgccg gcattaaccg agtaggctgt aatcatatcg
     4381 gtgccctttt tggcagcgcc ttttgcagtg ctattgaata gctcggcagc cctggcatca
     4441 atatccgcag tctcagccat gtacttatgc tcaagttcct tctgttttgc ctgcagatcg
     4501 ggagtcatga catttgcgcg gctgtacacg aaattggtta cttcattgaa tacccagaag
     4561 gcagcatcgg cgctgtaggt catgatgtcg ccatttccca ccgcaaatga gtggggaacc
     4621 gaggtaattc cgcaatacat cggtgtatag actgtatagt atgtatcatc aactccaaac
     4681 cagaggatac cgcctacagg gtcagggagc catgaacggc tctgggttac gaacgaaaag
     4741 cctgtctgct gtgttgatgt ggctctttca tgcagatact cctttccgtc aaccttccag
     4801 ctcatgggcc tccaccttac ggtattggca tacggacctg cgccaatatc ttttgtcatg
     4861 tccattggag tgttctggta atagtccctc ataagtccca taacatcttc caggctgagc
     4921 ttgttgtcag gcttgaccca gagaggcatt ctgttctcaa ggtcgtatcc catggcatag
     4981 ttgagatatt cacccatagt gctgttgacc ctgttgaatc ctgcccatac gcgtgcatcg
     5041 cagaaacgtg cggcgccaaa atcaacaggt gcataaacat cactgaagct gaagtctgca
     5101 tcggtgccgt cataccatcc gaagtcccgt gcaacagtca ctacatcagc agcataaact
     5161 gtctcaaccg aagggttgaa tatcttgtct atctctgccg atgtgattga tgtggtgccg
     5221 ttggcctgcg ggaaagtctg tatccttgcc tggttggcat ggccggagat gtaaccgtca
     5281 ggaaccaggc gggcaacaaa tgccattccc ttcttgccct gtcccttgcc aatcatctcc
     5341 atgatccaca cttcgttggg atcagatatg gagaatgatt cacccgaact gcaatagcca
     5401 tatttttcgg tcagtgtgac aatggtgtgt atggcctctc ttgcgttctt cgccctctgc
     5461 agcgtgaggt aaatcaggct tccgtaatca attaatccgt cgcgattgcg cagttcgccg
     5521 cgtccgccat aggtggtctc actgatagcc agctggtact ggttcatgtt accgactact
     5581 gagtaggtaa cgggtgcctg ttcgatggtg ccaaggaatc tgcctgaatc ccactcgtaa
     5641 acatcaaaca ttgttccggg tttatacttc atcgcgggcc agaagtaaag ctcgccataa
     5701 aggacgtgtg agtcggccga ataggtgatc atggtggaac cgtctttaga ggctcctttg
     5761 gtgatcagga agttggtgca tgcgtggctc ttctcggaaa taataaacat catgagaatg
     5821 aggctcagca tcagtgatgt ggttttcttc atgtttatag aattaattta attaattgta
     5881 ttataatcaa ttactgctac gttctcgttt cctgccgatc agggaattct caaaaatagc
     5941 atctttaatt aaaaaacaat accctttaaa atcataatta ccgaactgat ccgctcggca
     6001 tcggcgcttt taaggatata ttccgactgc agcctgggat cacccatata gatacctttt
     6061 ctgcggaagg tcatcatgct ttccctctgc cggcggccgg agaggtgata ttcccttgcg
     6121 ccggtgcctg ctgccaacag acctatattg tattcatcaa tgcctcctcc cggcattatg
     6181 attattcttc cagccgacca tgaaataagg cttttaatga gcgtggtgcc gtcgatggcg
     6241 cttttggcct gaccggaagt aagtattctc tccgcaccgg cggcaatcag atcctcaatt
     6301 cccttttttg cgtccctgca gaggtcgaat gcccggtgaa aggtcaggct catcggtgct
     6361 gcatattctg ccagaagggc cgttcgctcc acatctattg tcccatccct gttgagtatt
     6421 ccggtaacta ttccgtcagc accgttctcc ccggcaactt caatgtcacg ccgcatcaca
     6481 ctgaactcac tgtcactgta aaggaagtca ccgccgcgtg gcctgataag gacgtggatt
     6541 tttatccccg tgttgcgccg taccgcttcg ataagaccgg cactgggagt tactcctccc
     6601 tcggcaagtg cagaacacaa ttctattctt ccggctccgg cagcttcggc aacagtggct
     6661 gactcaacag agtcagcaca tatttctacc acaagagaca attcttttct atgaggcttt
     6721 gaaagtacat attatttgga taatattgtc tgagctaaaa catttaatca gatgaaacct
     6781 acactaatgg ttcttgctgc cgggatgggc agcagatatg gcgggaataa acagcttgac
     6841 caggtcgggc cttccggcga aacgattatt gattattcga tctatgatgc caggagggcc
     6901 gggttcggca aggtggtatt tgtcataagg cgtgatatag aggagcaatt cagggagact
     6961 tttgtaaaga gactcaggga tgtaatagag attgattacg tttttcagga tctcggaaac
     7021 cttcccggcg gatacaaggt gccggaaggg cgtcagaaac cctggggaac cagtcatgct
     7081 attatggtga cggaacccaa gattcacgag cctttcgggg taataaacgc cgatgactac
     7141 tatggagttg acgcctttaa ggtgctttac agtttcctca cagaggatac caatgacagg
     7201 aattattgca tagtgggtta ccgtcttggc aacactcttt ctgaccatgg tcatgtcaac
     7261 cgtggcatct gcagggtgag cagtgacgga ttcctggaac atattgtgga aaccagggga
     7321 atagagaaga ggcctgacgg tattttcgct ccggatgcgg aaggaaacgt taacaggtac
     7381 accggtgacg agattgtctc catgaacctc ttcgggttca aaccctcagt ttacaaatac
     7441 ctgaacatgg agttcaccaa cttcctgaag gaacgcatgt ctgacccgaa ggctgaactt
     7501 gatattccta catcccttga caaattcgta aagaaagggg agataaccat tcagattctg
     7561 agaagcaaca gccaatggtt tggtgtcacc tacagggagg acaagccatt cgtcgttgag
     7621 agcatcagga agatggttga ccagggaatg tacccggaaa aattgttttg acctgcacgc
     7681 atggcagtgt aatccgcata aatataatgt aacgaggggt ctgagacccc tcgctataca
     7741 ccctataatt ccatttcata aaaattcatt gatccaaata cgccaaatcc gctcatcaat
     7801 gtagaaattg tagtccggaa cttattaaag ttcagtgaaa tattcgagtt gccaatatat
     7861 gctgccatat tttgatcata tgcacagatc attaatcttg ttccggaatg atcaaaaggc
     7921 agtataatct caacatctgt tttcctatca ggaaatgtta caaggcgtga aactaataaa
     7981 tgattgtcat tatccgcgat ttggtatata tctaccatgc ctgtaagact atcaggaagc
     8041 aaagaaaaag atatcatttt cctgtcggtt attaaagtct caaggtctat ttgcggtttt
     8101 gaagggataa ttgtttcacc aattgcatca ggcatgccct catatttaca ggttaacctg
     8161 tatcgttcgc cggctcccgg tgaaaaatat gttgcagggc ggtatacagt gtcggttctt
     8221 acagtttgcg acggtagtaa agaaaacaca acagactccg tcaaactatc ctgaacaatt
     8281 ttatcaatct tcacaaatgc aggtttcaaa atgtcatagc cctcaagctc aagtgccgga
     8341 aaaaccctgt taacatatac aaacgatttg ttaaaccctt cggttgaatc agggcgaagc
     8401 aaaccaaaaa cattcaatcc ttcttcagta tgcttattta cgaattttgg ctgctggttc
     8461 aatccgatat agtactcaca ggaaaacagg agtaatgcca aacccaggga aacgatataa
     8521 atttgcttct ttttcatcat aatacaattt tgattttaat ggtattatgg aacccgcatg
     8581 tttgataatt atatacatac gtgtttatgt atgaaatata tgtgggtttc atatagttca
     8641 gaatttaatt gaataactga accccagtga aggaagataa tttgttccaa ttggcagata
     8701 tcttgagtca tcagaaccta catagagcgg aaagaaatat tctacatttc ttctaaagaa
     8761 tgtcagattg cgtaaagtca ctatcatgta agattctttt gcatggataa gttcagaaaa
     8821 at
//
LOCUS       DAOPBK010000108         8773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7512_length_8773_cov_4.471324, whole genome shotgun sequence.
ACCESSION   DAOPBK010000108 DAOPBK010000000
VERSION     DAOPBK010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7512_length_8773_cov_4.471324"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLA17_07335"
     CDS             <1..915
                     /locus_tag="PLA17_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85266.1"
                     /translation="LFDRITGSVDVYSRKTLDMLLAYPLSRTSGFSSITTNIGSIKNE
                     GIEFMLDGTLITGNNFEWSAGFNVAHNNSTILDLGKDEQFIPGNNRLVHKVGEHLYSF
                     YLYDYAGVNPANGDAMWYNENGELTNNYAQARRIIAGTPEPKLTGGINTKLAAYGVTL
                     DVNLEYKWGNLVSIEENRYANSDGYSWPNTGANTQLDYWTTPGQIARNPKPIANNTTS
                     SNGYRSTRWLYDGSYLRLKNITLSYNLPRAVVNKMKMQNLRVYASAVNLYTFHNVDFW
                     DPERGVDGCGFGIYPMSKKIIGGIEVSF"
     gene            935..2380
                     /locus_tag="PLA17_07340"
     CDS             935..2380
                     /locus_tag="PLA17_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK85267.1"
                     /translation="MKTKHLILLMVTFAVLFTACEDFLVKEPRLSQSNELTLSTYKGL
                     DNATGGVYSQLCGYAWYGSQFVITADMKGGNAKRGSVISGRYTDEYLWNNTPASTSGL
                     WATAYSTIAKANNVLEVIDGGFTEAGVEEAQLNVLRGECLFMRGLAYFDLARMYCQPY
                     SAGTTNLGVPVVLVTENGYPARNTVGETYDRVVADLKDAIALLPVTNKRGDDAAYATK
                     VAAQALLAKVYLYMENWAEAASYATTVIGTAGLSLFTADDYTTWDNKGYWGSGGPGKE
                     IIFQVDGSEGNSSHPWWDAISYMMDPGGYGDCAASLDVLNLYEAGDVRADLFLQPDDY
                     PGEYWSLKYPGRLGRTPHREFNVPVLRLAEMYLIRAEAILNGASVAGATALDDYNAIR
                     TNRGLAAAGAVTLSDIYAERRRELCFEGNELFDLARTQRSLVRNDYTGLSNKDIPFTA
                     GGTALVNYKWAMPIPQAEIDANVNCEQNPGY"
     gene            2398..3276
                     /locus_tag="PLA17_07345"
     CDS             2398..3276
                     /locus_tag="PLA17_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85268.1"
                     /translation="MKKIISNLLILTVVMLMTTSCDDKYPLIFDDSSTIVGMSQATLS
                     VKEDATGSFTIYLGAAQGTEATDVTLSVSVEGIGKPAVEGTDFTLSTKNLNLPVGLAS
                     VTVTPVNNDTFNGNKQFRVSIVSNSKNYPVAVQNSILVTIVDDEHPLKTWLGTFNVTA
                     VSYGKPGAWDEDWTITTSAVESDNNKLNVVGIAGTSTPVVATLNTTDMTIEMKSPSAL
                     GETYGYSSGSLYYATDEILAISSGYLTAGMLEAASSYKITGTIEADGTIKIDRMCIVI
                     DAYVYAWDCFNTTWTK"
     gene            3389..5056
                     /locus_tag="PLA17_07350"
     CDS             3389..5056
                     /locus_tag="PLA17_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPK85269.1"
                     /translation="MLRRLFISLLFLLPVAAGAQVVTRSQMAETANAFINGYLGSAVH
                     KVSGIRPMSVAGGDAIFVIDLLPEGWLLMSGDYSALPVLAFSLTGTFTEPSESEKDNR
                     FVFLSGYADQLKLSVTAKSSSRDPRWDPAYYFNKSAVASTAEVIVSPLIKVTWNQNKG
                     WNRFCPEDPDGPGGHVYVGCVAVSMAQAMSVFGIPEHGKGARQYDHPTYGSIYADFSS
                     ATYDWANMKLAMPDDNNALILFHCAVSVDMDFSPAGSGTLTSAAAASALKEFFFFSKR
                     IAFKKRGTNTAEWKALLDENLLAGRPIIYSGFPGTGASGHAFNIDGVFRSNYYHLNWG
                     WTGVDDGYYTIDNLKPGGSDFTKDHTAILGIQPYYYPTDVALSDTVVLLSNPPGTAIG
                     KFTVVDEATDNSYDIVLECDSTLTGTEWVPDYYLDGDSLRMSRSFEVSDGPADTITFI
                     VSDAHGNNIRAARLLRLTASLAAGDGWQEDAFTVWPVPFTDRFMIELPPESNRISIRN
                     ISGAEMAGMVPESDIITISGAGWPSGIYIVTVTTARGKQHSKTIVRY"
     gene            5154..5819
                     /locus_tag="PLA17_07355"
     CDS             5154..5819
                     /locus_tag="PLA17_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPK85270.1"
                     /translation="MSVKIRLARKGRKKQAFYHVVVADSRSPRDGRFIEKIGTYNPLT
                     DPATIEINFERALEWLQNGAQPTDTCRAILKYKGVMMKKHLLEGVRKGAFDEAEADRR
                     FNEWLKGKEEKIELKRSGLEKKGEELRKKKLEAEKAIKEARAEELAKRAAALAAEAEA
                     AAKAAEAAEAPEESAAEDAAGEASAQTETEAPAEAETEAPAEAETEAPAAEAQEDTAE
                     KTE"
     gene            5846..6340
                     /locus_tag="PLA17_07360"
     CDS             5846..6340
                     /locus_tag="PLA17_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85271.1"
                     /translation="MRTIGRITKTHGFDGAVVVKSRRAITGDPEQGEPVFVITEGIPV
                     PFFTREAFLPAPDTMIISFDDYLTAESVAHLRGCEMLSEGAGESGNDLMALEGFTLTD
                     TNTGLSGIIDDLIQNPGQVIAVAVIEGRRVYIPLHPDLIVSVDRRHKTITMTLPEGLV
                     SLND"
     gene            6426..7121
                     /locus_tag="PLA17_07365"
     CDS             6426..7121
                     /locus_tag="PLA17_07365"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPK85272.1"
                     /translation="MKRILIFSLLTIFAARASFAQTTCTEKRQLSGFDEVTFAVSGEV
                     LISLGRDFSVELEGDRDYIREIITRVDDDELIIRRDKWFDFRNKKVTVRITMPALDGI
                     SILGSGSLTVIDPLRGDELDVAISGSGKAFLRDVSLREVECDISGSGSLNLTGNGTIR
                     KLEISISGSGDYVGDDTTVGIMDARISGSGSCDCHVTEMLRASISGSGNIRYSGDPKI
                     DATVTGSGKVLMK"
     gene            complement(7122..7718)
                     /locus_tag="PLA17_07370"
     CDS             complement(7122..7718)
                     /locus_tag="PLA17_07370"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPK85273.1"
                     /translation="MTVQRKGMKSEFGTDLAAALKVLRAGGVILYPTDTIWGLGCDAT
                     NAKAVRKIYEIKHRAENKSLIILVNSVAMLERYIENPPGVAIEMAELSEKPLTVVYDR
                     GRNLAEGVASFDGSVGVRICAEPFCDALIETLRKPLVSTSANISGGSSPVIYDEISEE
                     VKAAADYVCLWRQDDRSRATPSPVIRISGNGIVKILRR"
     gene            7819..>8773
                     /locus_tag="PLA17_07375"
     CDS             7819..>8773
                     /locus_tag="PLA17_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HPK85274.1"
                     /translation="MMRKMLAFVILAASAMLVSAQDKTGYQLPPEAIVSLLDAPQTPS
                     VILSSDNQTMLLLYRPGLPSIADLSQPELRLAGIRFNPSTNGPSRVQGMIRVSSIKRD
                     GTQLGDLKGLTSGASVGGVTWSPDNKRFAFTNTTDNTIELWVADAATMTAQKVDEGLN
                     CVLQRDITWLPDGSLIYLRTLRDRGPAPESSRVPAGPSVQESEGVRGDLRTFQDLLQS
                     VDDEKIFEYYATSELVRWDGTSAASFGRPGMITGVSPSPDGNWLLVSIIEKPFSYLVP
                     YYHFPSRTEIWDLQGKMVRLMLETPLRESLPRGYDVVIPGPR"
BASE COUNT         2318 a         2131 c         2194 g         2130 t
ORIGIN      
        1 ttatttgacc gcatcaccgg ttcagttgat gtgtactcac gcaagaccct ggatatgctt
       61 cttgcctatc ccctgtcacg tacatccggt ttcagctcca taacaaccaa catcgggtca
      121 atcaagaacg aaggtattga gtttatgttg gacgggacgc tgatcacagg caataacttt
      181 gaatggtctg ccggtttcaa tgttgcccat aataacagta caattcttga ccttggcaag
      241 gatgagcagt tcatccctgg caataaccgc cttgtacata aggtaggtga acacctgtac
      301 agcttctacc tgtatgacta tgccggtgtt aacccggcca acggtgacgc catgtggtat
      361 aatgagaatg gtgaactcac caacaactat gctcaggcca gaagaatcat agccgggaca
      421 cctgaaccca agctgaccgg tggtattaat accaagctgg cagcctatgg cgtaacactt
      481 gacgtcaacc ttgaatataa gtggggcaat cttgtatcca ttgaggagaa ccgttatgcc
      541 aactctgacg gatacagctg gcccaatacc ggtgcaaata cccagctcga ttactggact
      601 acacccggcc agattgcaag aaacccgaaa ccgatagcca acaacactac cagttcaaac
      661 ggctaccgca gcacaagatg gttatatgac ggctcatacc tgaggctgaa gaatatcacc
      721 ctctcataca acctgcccag ggcagttgtt aacaagatga aaatgcagaa cctgagggtc
      781 tatgctagcg cagtgaacct gtacaccttc cataacgttg acttctggga tccggaaaga
      841 ggcgttgacg gatgcggttt cggtatatat ccgatgtcca agaagatcat aggtggcatt
      901 gaggtatcat tctaacaaat aaatcgatga cgctatgaaa acaaaacact taatactttt
      961 aatggtaacc tttgcagtac ttttcactgc ttgtgaggat ttccttgtca aggagccccg
     1021 gttatcccag tcaaacgagc ttacactttc cacctataag ggccttgata atgccactgg
     1081 tggtgtatat tcccaactgt gtggttatgc atggtacgga tcacagtttg tgatcacagc
     1141 tgacatgaag ggtggtaatg ccaaaagggg ttctgtaata tcaggacggt atactgatga
     1201 gtatctgtgg aacaacactc ctgcatcaac atcagggctt tgggcaacag cttattctac
     1261 aattgcaaag gccaataacg tgttggaagt aattgacggc ggtttcaccg aggccggcgt
     1321 tgaagaggct caactgaacg tgcttagggg cgagtgcctc ttcatgagag gtcttgctta
     1381 ctttgacctt gcacgcatgt attgccagcc atattcggca ggaaccacga acctgggtgt
     1441 accggttgtg cttgtaacgg aaaacggtta tcccgcccgt aatacggttg gtgaaactta
     1501 tgacagggtt gtagctgacc ttaaggatgc tatcgctctc ctgccggtca ccaacaagag
     1561 aggagatgat gctgcatacg caaccaaggt ggctgctcag gcgcttcttg ccaaggttta
     1621 cctctatatg gagaactggg cggaagctgc atcatatgcc accacggtaa ttggtactgc
     1681 agggctttca ctcttcaccg ccgatgacta caccacatgg gacaataagg gttactgggg
     1741 cagtggtggc ccgggtaaag aaattatctt ccaggttgac ggctcagaag gcaactcctc
     1801 acatccctgg tgggatgcaa tatcctatat gatggatccg ggcggctacg gcgactgtgc
     1861 tgcatcactt gatgtactca acctttacga agccggtgat gtgcgtgctg acctgttcct
     1921 ccagccggat gattatcctg gcgaatactg gagcctgaaa tatccgggac gtcttggaag
     1981 aacaccccac cgtgagttca atgtgccagt tctccgtctg gcagaaatgt accttatccg
     2041 tgctgaggct atccttaacg gagcttcagt agcaggagca accgcactgg acgactataa
     2101 cgcaatccgt acaaatcgcg gactggcagc cgcaggtgct gtcaccctga gtgatatcta
     2161 cgctgaaaga agaagagaac tgtgcttcga aggcaatgaa ttgttcgatc ttgcacgcac
     2221 acagcgctcc cttgtcagga atgattatac cgggctttca aacaaggata tcccattcac
     2281 cgcaggcgga acagctcttg tcaattataa atgggctatg cctattcctc aggcagagat
     2341 tgacgctaac gtcaactgcg aacagaatcc gggttattaa cattaaactc gtaaattatg
     2401 aaaaagataa tttccaactt actcattctc acagttgtca tgttaatgac gacctcgtgc
     2461 gatgataaat atcctttaat ttttgatgat tcaagcacca tcgttggaat gagccaggcc
     2521 actctttcag tcaaggaaga tgcaacaggt tcattcacaa tctaccttgg cgctgctcaa
     2581 ggaacagagg ctacagatgt cactctttct gtctctgttg aaggaatagg caaaccggca
     2641 gtggagggaa cagacttcac cctttcaaca aagaatctga atctgcctgt aggccttgca
     2701 tcggtaacgg ttaccccggt caataatgac acttttaacg gcaacaaaca gtttagggtc
     2761 tcgattgtct ccaacagtaa aaactatcct gtagcggttc agaattcaat cctggtaacc
     2821 attgttgatg atgaacatcc gcttaaaaca tggctgggca catttaatgt tactgctgtc
     2881 agttacggaa aaccaggtgc ttgggatgag gattggacaa tcaccaccag tgcagttgaa
     2941 tctgataata acaagcttaa cgttgttggt attgcaggaa cctcaacccc ggttgtggca
     3001 accctcaaca ctactgacat gacaattgag atgaagtctc cgagcgccct tggggaaacc
     3061 tacggctatt ccagcggatc actctactac gcaactgatg agattcttgc aatttccagc
     3121 ggttacctta ctgccggcat gcttgaagct gcttcatcat ataaaattac tggtaccatt
     3181 gaggcagacg gaaccatcaa gattgacaga atgtgcattg ttattgatgc ctatgtctat
     3241 gcatgggact gctttaatac aacctggaca aagtaaacag agaccggaca ggtaattaac
     3301 tgaattaaga gggtgttttc catttaccgg gaacaccctc tttttcacct ctgtaaattt
     3361 cggtatattt gcacctttgt ttcaaacaat gctaagacgg ttattcatat cacttctctt
     3421 tctgctgccc gttgcagcgg gtgcacaggt tgtcaccagg tctcagatgg cagaaacggc
     3481 caatgccttt ataaatggat atcttggaag cgctgttcat aaagtatctg gtataagacc
     3541 aatgtctgtc gcaggtggag atgcaatatt tgtgattgac cttctgccgg aaggctggct
     3601 ccttatgtca ggagactact ctgcattgcc ggtattggca ttctccctta ccggaacttt
     3661 tactgagcca tctgaaagcg aaaaggacaa caggttcgtc ttcctttccg gatatgcaga
     3721 tcaactaaag ctttctgtta ccgcgaaaag ctcttcaagg gatcccaggt gggaccctgc
     3781 atattacttc aataagtcag ctgttgcaag cacagcagag gtgattgtct caccgctgat
     3841 caaggtcacc tggaatcaga ataagggctg gaaccgcttc tgtccggaag acccggacgg
     3901 ccccggagga catgtttacg tgggatgcgt ggctgtgagc atggcacagg caatgagtgt
     3961 cttcggtata ccggagcatg gtaaaggtgc cagacaatat gatcacccca cgtacggaag
     4021 tatttatgcc gacttcagtt ctgccaccta tgactgggca aatatgaaac ttgcaatgcc
     4081 tgatgataat aatgcactga tcctgttcca ctgtgccgtc tcagtagaca tggatttcag
     4141 ccctgcggga tcaggtaccc ttacgtctgc cgccgcggca agcgcactga aggagttctt
     4201 tttcttttcc aaaaggatag ccttcaaaaa acggggaacc aatactgctg aatggaaggc
     4261 tctgcttgat gagaatcttc ttgccggaag gccgattatc tattccggtt tcccgggaac
     4321 cggagcatcg gggcacgcat tcaatatcga cggagtattt agaagcaact actatcacct
     4381 caactgggga tggaccggtg ttgatgacgg ttactatacc attgacaacc ttaaacccgg
     4441 cggctccgat tttacgaagg atcatacagc cattctgggt atacaacctt attattatcc
     4501 cactgacgtg gccctctctg acaccgtggt actgttaagc aatccgcccg gaacggcaat
     4561 aggcaaattc accgtggttg acgaagccac tgataactcc tatgacattg tcctcgaatg
     4621 tgactcaacc ctgacaggaa ctgagtgggt gcctgattac tatcttgacg gtgactcgct
     4681 gagaatgtca cgttccttcg aggtcagcga tggcccggca gacaccataa ctttcatagt
     4741 gagcgatgcg cacggaaaca atatcagggc tgcaaggctg cttcggctca cagcttcgct
     4801 tgctgccggc gatggctggc aggaggatgc tttcacagtc tggcccgtgc ccttcacaga
     4861 ccggtttatg attgaacttc ctcccgaatc aaacaggatt tcgataagaa atatcagcgg
     4921 cgccgaaatg gccggaatgg ttccggaatc tgacataata acaatttccg gcgccggctg
     4981 gccttcagga atctatattg tcaccgtcac aacggccagg ggaaaacagc attcaaaaac
     5041 cattgtcagg tattgagagg caaattttaa tatgcagaac tgtcggcagg taaaaattaa
     5101 cttgtatatt tgcaccctcg tttaaaaaga gtaacaataa attaattctt taaatgtcag
     5161 taaaaatcag attagctagg aaaggccgca agaagcaggc cttctaccac gtggtggtag
     5221 cagatagcag gtcgccacgt gatggcagat tcattgagaa gatcggaact tataatccgc
     5281 tcactgaccc ggcaacaatt gaaatcaact tcgagagggc actggaatgg ctgcagaatg
     5341 gtgcgcaacc tacggacacc tgcagggcaa tcctgaagta taagggagta atgatgaaga
     5401 agcacctgct tgaaggagta agaaaaggtg cttttgacga agcagaagct gaccgccggt
     5461 ttaatgaatg gctgaagggc aaggaagaga aaattgaact gaagaggtca ggcctggaga
     5521 agaagggcga agagctgcgc aagaagaaac ttgaagctga aaaggcaatc aaggaggcca
     5581 gggcagaaga gctcgccaaa agggcggcag cccttgccgc tgaggcagag gcagcagcaa
     5641 aagctgctga agcagccgag gctcctgaag agagcgctgc cgaagatgca gccggagagg
     5701 cttcggcaca gacagagact gaagcgcccg cagaggcaga gacagaagct cccgcagagg
     5761 cagagacaga agctcccgca gctgaagctc aggaagatac tgcagaaaag accgaatagg
     5821 gtcatctgcc ccctgccccc tgattatgag aaccattggc agaataacaa aaacccacgg
     5881 cttcgatgga gccgtggtag tgaagagcag gagagccatc acaggggatc cggaacaggg
     5941 agaaccggta ttcgtcatta ccgaagggat accggttccc ttttttacac gggaagcatt
     6001 tttacctgcc cccgatacta tgataatatc gtttgatgat tacctcactg cagagtctgt
     6061 ggcgcacctc cgcggatgcg agatgctttc tgaaggcgcc ggagagtcag gtaatgatct
     6121 catggcgctg gagggattca cactgactga tacaaacacc ggcttgtcag gcataataga
     6181 tgatttaata cagaaccccg gccaggttat tgccgtggct gttatagaag gccggagggt
     6241 gtatatccct ctccaccctg acctgattgt ttccgttgac cgcaggcaca agacaatcac
     6301 gatgaccctc ccggaaggtc tggtaagcct taacgactga caatcctgcc tcttttttga
     6361 tttacagtgc agcatagaac attttgcgcc gtctttctgt tgtaaccaga taaaacggag
     6421 cagctatgaa aagaatcctg attttctcat tattaacgat tttcgccgcc cgggcttcat
     6481 ttgctcagac aacctgtacc gaaaagagac agctgtcagg ctttgatgag gtaacctttg
     6541 ccgtttcagg cgaagtgctg atcagcctcg gcagggattt cagcgttgag ctggaaggag
     6601 acagggatta catcagggag atcattacca gagttgacga tgatgagctg ataatcagac
     6661 gggataaatg gtttgatttc agaaacaaaa aggttactgt aagaatcacc atgcccgccc
     6721 tcgatggtat cagcattttg ggctcaggga gcttaacggt aatcgatcct ctcaggggcg
     6781 atgagctgga tgtggccatc agtggcagcg gcaaggcatt tctccgtgat gtgtcactca
     6841 gggaagtgga gtgcgacata tccgggtcag gcagtctcaa tctcaccggg aacggcacca
     6901 tcaggaaact ggaaatctca atttcaggtt cgggcgacta tgtgggggat gacaccactg
     6961 ttggtatcat ggatgccagg atttccggca gcggcagttg cgactgccat gtgactgaga
     7021 tgctgagagc ctctatctcg ggcagtggca atatcagata ctcgggtgat ccgaagattg
     7081 atgctactgt tacaggctca ggcaaggtgc tgatgaagta atcatctgcg cagaatcttt
     7141 acaataccgt taccggatat tctgatgacc ggagagggtg tggccctgct tcggtcatcc
     7201 tgtctccaga ggcatacata atctgccgcg gccttaacct cttcacttat ctcgtcatat
     7261 attacggggc tgctgccgcc gctaatgttt gccgatgttg ataccaaagg cttccgaagc
     7321 gtttcaatga gcgcatcgca gaacggttcc gcgcatatgc gcacacctac tgacccgtcg
     7381 aaagaggcaa ccccctcagc caggttcctt cccctgtcat agaccaccgt caggggcttc
     7441 tccgagagct ctgccatctc tatggccact ccgggagggt tttcgatata cctctcaagc
     7501 atcgccacag agttcacaag tattataagg ctcttgttct cagctctatg ctttatctca
     7561 taaatcttgc gtacagcctt cgcgttggtg gcatcacagc ccagccccca gatggtgtca
     7621 gtggggtaaa gtattacccc gccggccctg agaaccttca gggcagcagc cagatcggtt
     7681 ccgaattcac tcttcatccc tttacgctga acggtcatac acagtttttt ataaagttaa
     7741 aaaacaataa taactcttat ttgctttctc tatctgaaat cagaagatta aatttgagac
     7801 agaccaaaaa gaaccattat gatgcgtaaa atgcttgcct ttgtcatcct tgctgcttca
     7861 gcaatgctgg tcagcgcaca ggacaaaacc ggttatcagc ttcctcctga agctattgtc
     7921 tcactcctgg atgctccgca gaccccgtct gtaattctta gctctgacaa tcagacgatg
     7981 ctgttgttat atcgtccggg gttaccatcc atcgccgacc tgtcgcagcc tgaactgcgt
     8041 ctggcaggga tcaggttcaa tccttcgacc aatggtccca gccgtgtgca gggaatgatc
     8101 cgtgtctctt cgataaagag agacggaacg cagttgggtg atctgaaagg tcttacctcc
     8161 ggtgccagtg tgggtggggt tacatggtca cctgacaaca aaaggtttgc cttcaccaac
     8221 accactgaca atacaattga actatgggtt gccgatgctg caacaatgac ggcgcaaaaa
     8281 gttgatgaag ggctcaactg cgtccttcaa agggatatta cgtggctgcc tgatggttcg
     8341 ctgatatacc tgcgcaccct gcgcgacagg ggaccagctc ccgaatcctc cagggtgccg
     8401 gcgggtcctt cggttcagga aagcgaagga gtcaggggag atttacggac attccaggac
     8461 ctcctgcaga gtgttgacga tgaaaagata tttgaatact atgccacttc cgagctggta
     8521 agatgggatg gcacgtcggc agcctccttt ggccgtccgg gaatgatcac aggtgtttcg
     8581 ccctcgcctg acggtaattg gctcctcgtc tccataattg aaaagccatt ctcatacctg
     8641 gttccgtatt accatttccc gtcgcgcact gagatatggg accttcaggg aaagatggtc
     8701 aggctgatgc ttgagactcc gctccgcgaa agcctgccca ggggatatga tgttgtcata
     8761 cctggtccca gga
//
LOCUS       DAOPBK010000109         8740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7543_length_8740_cov_3.824180, whole genome shotgun sequence.
ACCESSION   DAOPBK010000109 DAOPBK010000000
VERSION     DAOPBK010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7543_length_8740_cov_3.824180"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4492)
                     /locus_tag="PLA17_07380"
     CDS             complement(<1..4492)
                     /locus_tag="PLA17_07380"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85275.1"
                     /translation="MQQTLIIEFDRAVTAAGTSAGWTITVGGANVVMVGNPVPSGNFL
                     RISLPSSITYANRNSVLVSYNPAVGSMSLTGGVEPNFTNVQAVNNYISIAADFTNGLY
                     GELPPSDICSPVLDVQVESNIIMSRRYRNSIHYALPKIWILWEYPNAFPRTLAFYTEV
                     GGTGSGIFRSVEAYAGYPDNTSNCTWDVGIYPYLYNTGTLQPNLYVTVNQVVIQLPNY
                     KRDNGTPPQGTGNLGIDPPVDDPRTLFCVGDDITAFIFEDATVFDCQLPVEPDLPNIY
                     DRHVQWVYGTHTTAGIPNVFIDVNGTMVQVTDNDGVAITGTWYVNPDGTPNPGGYTTP
                     SGFFEGPVIQYEWDFFTRLLITPMAQTYPIYHTGDFVNDKANDVFDVTLRNWGPCNPW
                     DGGNPFTHLDAVTEFSRLRLIESPPLPTAPDVFICFGGSTTLTAVRNGAPNPGVLHWY
                     DDAALTHEVATGNTYTPSVSAVNTYTYYVREISGNTGNCPGPAEEVVLTINPIPNTPT
                     VTRTGSDFCYDGIQSVTLTADPNSPPVVSSYQWYRNGTAISGATSSTLVLSQIAHSGT
                     YTVRAFGVAPTYCPGPLSDPISVTIGQPATVNAGPDQNICSNTPASLAGTIGGSASSA
                     TWTTSGTGSFGNATGLSTTYTPSAADISSGSVTLTLTTNNPTGPCPAVSDELVLTIVR
                     APQVYAGADAAICQGTAYTVNDATASNYATLTWTENGTGAITAGQGTLTPTYTPGPGE
                     ANATVTLTLRAEGNSPCATVTDTKTLWIDRMPVATVGPIQEICNNTSASLAGNSPASG
                     TFGEWTFINNLVWQETFSESPQYATSGVQWSTSGITPDADTYFRVENNRMVGRDLDAE
                     AVWRSSSIPIASVSPVKVSVNLQETGTLENDDYKRVYYSIDGGPETFFTTNGNLNDDF
                     GNLTASVTNLSGNNLRIIIRCRNDNPNEYYFFDDIVVRQVTAQTEPTITTITSPTSTV
                     SGLWQGDNQFRWSVFSTHGGCDSASAVYTIRRDVSPAAANAGLPQTFCETSSTVMAAN
                     AATNGGTGTWMLISGSGTVAEPNNPTSAVTGLGYGVNTFRWTISSALGICSNSSSTVT
                     ITRNRNPIDLTGNVTILKNPVCYNTPGQLRITGTEADVKYYLRTGETDGSFVQGNGGT
                     ITLTTPNLTAATTFQIHAIKDGTGCDIIFGSYTINVNPEFTLAQLIETHNICAGATTT
                     ISVALTGGTSPYTIVWNDGSDHTINNYVSGTSITVGPYPAGSTTITLTSVTDNNLCVP
                     ASLGNPITITVGSTPVSATLTGSGDACVGSTSSLTVTVTDGVSPYDIVINGVTYPDLT
                     SGGSIDLGQLPVGTYTYNLTSVIDACGNPVPAGGLPPAYSFSINEIPSASGTTNDAPE
                     ICSNGTTDIALRSTVANSTYEWTVSSAPATTWTTGKAPAGGTGGMGTVIEQNLEHESY
                     LPITVTYTITPKGPAPTYCVGPPVTRQVIVNPVARISDKTASICSGSTFTVTPANGGS
                     EIV"
     gene            complement(4858..5754)
                     /locus_tag="PLA17_07385"
     CDS             complement(4858..5754)
                     /locus_tag="PLA17_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85276.1"
                     /translation="MDFTLRKYRKLLTALKGCSIPFTGFSDLPEDGYKSFVVLRHDVD
                     KRPENALDMARAEADEGIRASYHFRAPGGPLPYQTISAIAALGHEIAYHYEDLSVVAG
                     RRAAVRGFVDLSLVEAAFSRFRINLADLRGIAPIRVISMHGSPLSAVDNRLLWKYYDY
                     RSEGIVCEPYFDVDVSAVLYLTDTGRSWNSDRSVMRDRGLTAGERMAIGSYDDWKVRP
                     VRGSLMDMTAKGLQLRGKFRIRKTDEIIEMALSRVLPSGVIINTHPQRWSDDILEWFS
                     ELILQNLKNPVKAFFRNTKYFE"
     gene            complement(5767..6846)
                     /locus_tag="PLA17_07390"
     CDS             complement(5767..6846)
                     /locus_tag="PLA17_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HPK85277.1"
                     /translation="MNLLFDIGHPGQVHLLRNAISILKSRGHEVTVTVKEIPNAQKLL
                     DAYGISWLPLGRKYDSLLLKGFSQARYNYRLWKIAQDRKIDIAVGSSITIAHASLFHR
                     MKSILLDDDDAAAVRLFSLFAHPFADTVLSPSALAHQRKHRRDVVYAGTHELFYLHPS
                     RFTPDPSVAEKAGIDLSKPYFIARFVSGKAYHDKGERWITLEQKLRFVRMLEHYGNVY
                     ITTERTIEPELEKWQLRIAPELIHHLLSFATMFVGDSQTMTSEAAILGTPALKCNSFA
                     HKLSVPNMLEDKYDLCYAFQPEEFILMLKKAEELLGMADLKQRWIKKRERFLSDMIDP
                     TAFLVSFIENYPSCGHNHHRKENLV"
     gene            complement(6843..7676)
                     /locus_tag="PLA17_07395"
     CDS             complement(6843..7676)
                     /locus_tag="PLA17_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HPK85278.1"
                     /translation="MEQIIIILACFLLSSAIFVFWVAKGGFNKKYLYTDNLLLGSAGS
                     ILTILVHRILPIPFWVVVPFVSGFIILILFVLLFFYRFFRNPSRIIPGGPDDLVSAAD
                     GRVIYIKELEVNQMPVTVKKMRIAELSEITKTDLLERPCYLIGIAMTLFDVHMNRSPL
                     DGKITFIKHTPGTAIGLNTPSSTVTNERNTVIIERADGIKTGIVQIAARGVRRCIVTA
                     NVGDPVKRGEIIGKIRWGSQLDMILPRNSEILVREGEQVYAGRTVIAKLSGNHDNSGR
                     K"
     gene            complement(7692..>8740)
                     /locus_tag="PLA17_07400"
     CDS             complement(7692..>8740)
                     /locus_tag="PLA17_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85279.1"
                     /translation="RYSRYCTGFFYSPPFDSDDLALFLLDLARREGLEGWLLLPSNDH
                     AVYTIARNRDALGSVFKLITPGTDVIDLIYNKKNLLDMATELGVAIPTTFYFNRTDEK
                     IPADLKFPVITRGRFGLSFFKAMKAKALMAETTEELREQLSHIDRVIGPDKTLTQTVI
                     SFDGTNRTVSFTAFCTDGEIRCWWAGRKLREHPWRFGTATYAESIECEECLESTTRLI
                     RKLNYCGICEVEYIRDPTDNNYKLIEINARTWLWVGLAKACGVDYALMAYRYVNGLEN
                     TFPDDYVKGVKWINYLTDTWFSLGAMFSGRMKPGEYFTSFKGKRIPAVFSWRDIIPSF
                     ALVLLSFYIARKRR"
BASE COUNT         2327 a         2103 c         1975 g         2335 t
ORIGIN      
        1 gaactatttc tgagcctcca tttgcagggg tgaccgtaaa ggtactgcca ctgcaaattg
       61 aagccgtctt atcagatatc ctggcaaccg ggttgacaat gacctgcctt gtgactggcg
      121 gtccgacaca ataagttggt gccggccctt tgggagtaat agtgtatgtt acagttattg
      181 gtaaataact ttcgtgttca agattttgtt cgataacggt ccccattccg ccagtccctc
      241 ctgcaggagc cttgccggtc gtccatgttg ttgcaggagc agatgacacc gtccactcat
      301 aagtagaatt cgctactgta gacctgagag caatatcggt agttccgttg ctgcatatct
      361 ccggagcatc gttcgttgta ccgctcgcac tggggatctc gttgatgctg aatgaatatg
      421 ccggtggcag tccgcctgcc ggaacaggat taccgcatgc atcaataaca gaagtaagat
      481 tataagtata agtccccaca ggaagctgtc caaggtcaat gctgccacca gaagtaaggt
      541 cggggtaggt tacaccatta attacaatat cataaggaga cactccatca gtcacggtga
      601 ctgtgagtga acttgttgat ccgacacatg catcacctga tccggtaaga gttgctgaga
      661 ccggtgtgga acctactgta atcgtgatcg gatttcccag gcttgccggc acacaaaggt
      721 tgttatccgt gactgaagtc agtgtgatgg ttgtgctgcc tgccgggtac ggacctacag
      781 taatgctggt tccgcttaca taattattaa tagtgtgatc agaaccgtca ttccatacaa
      841 tagtatacgg acttgttccg cccgtcagag caaccgaaat ggtggttgtc gctccggcac
      901 agatgttatg ggtttctatt agttgtgcaa gggtaaactc cgggtttaca ttgattgtat
      961 aggatccgaa gatgatatcg catccggtgc catcctttat tgcatgaatc tggaatgtgg
     1021 ttgctgctgt caggtttgga gtggtgagag taattgtgcc tccgttcccc tgaacaaatg
     1081 agccgtctgt ctctccggtt ctgagataat actttacatc tgcctcggtt ccggtaatcc
     1141 gtaactgacc aggcgtattg tagcagaccg gattctttaa tattgtcaca ttgccagtca
     1201 ggtcgatggg attccggttg cgggttatag ttacggtgct gctgctgttt gagcatattc
     1261 cgagagcact ggatatagtc cacctgaagg tattgactcc gtagcccagg cccgtgacgg
     1321 cagatgtggg attattgggt tctgccacgg tacctgatcc gcttatgagc atccaggtcc
     1381 ctgtgcctcc atttgtggct gcattagcgg ccatcacggt actgctggtt tcgcagaatg
     1441 tttgcggcag tccggcattt gctgctgccg gggaaacatc tctccttata gtatatactg
     1501 cagatgcgga atcgcaacct ccatgagttg aaaagacaga ccatctgaat tgattgtcac
     1561 cctgccacag ccctgacacg gttgatgtcg gactggtaat ggttgtgatt gtcggttcgg
     1621 tctgtgccgt gacctgtctt acaacaatat catcaaaaaa ataatattca tttggattat
     1681 catttctgca tctgataatt atcctgagat tgttaccaga gagattggtt acagaggcag
     1741 tcaaatttcc aaaatcatca ttgagattgc cattggttgt aaagaaggtt tctggtccgc
     1801 cgtcgatact gtagtatact cttttatagt catcgttttc caaggtgcct gtctcctgga
     1861 gattgacact taccttcaca ggagatacag aggcaatagg tatggaactt gaccgccata
     1921 ctgcttcggc atcaagatcc cttcccacca ttctgttgtt ttcaacccgg aaataggtat
     1981 cagcgtcggg agtgatacct gaagttgacc attgtactcc tgaagtggca tactgcggac
     2041 tctcagaaaa agtctcctgc cataccaggt tatttatgaa tgtccattcg ccaaaggtac
     2101 ctgaagcagg tgagttacca gcaaggctgg cgctggtatt attgcatatc tcctgaatgg
     2161 gacccacggt ggcaacgggc atacggtcaa tccacagggt ttttgtgtct gtaacagtcg
     2221 cgcagggtga attaccctct gccctcaggg tgagggtaac cgtagcatta gcctcaccag
     2281 gaccaggtgt ataggtcggt gtgagtgtcc cctggcctgc agtaatcgca cctgtgccat
     2341 tctctgtcca ggtcagagta gcataatttg atgctgtcgc atcatttacg gtataagcag
     2401 taccctgaca tattgcagca tcggctcctg cgtaaacctg tggagccctg acgattgtca
     2461 gaaccagttc atcagagact gccggacatg gtccggttgg attattggtt gtcaatgtga
     2521 gagttacact ccctgatgaa atatcagcag cacttggagt atatgttgta gacagtccgg
     2581 ttgcatttcc aaagctgcct gtacctgagg tggtccaggt ggcagaagag gcagaaccgc
     2641 ctatggttcc ggccaatgaa gccggtgtat tggagcatat attctgatcc ggacctgcat
     2701 tcactgtagc gggctggccg atggtgacac ttattggatc tgacagaggc cccgggcaat
     2761 aagtcggagc aacaccaaaa gcccgaactg tatacgttcc tgaatgtgct atttgtgaca
     2821 ggactaaagt actcgacgtt gcccccgaaa tagcagttcc attcctgtac cactgatatg
     2881 aagagactac agggggcgaa tttggatccg ccgtgagggt tactgactga atgccgtcat
     2941 aacaaaaatc ggaaccggtt cgggtcactg ttggggtgtt tggtatgggg ttgattgtca
     3001 gaaccacctc ttctgccgga cccgggcaat tgcctgtgtt gcctgatatc tccctcacat
     3061 aataagtata tgtatttaca gctgaaacgg aaggagtata agtgttgcct gtagctacct
     3121 catgagtcaa tgcagcatca tcataccaat gaagcactcc cggatttgga gcgccattcc
     3181 ggactgcagt gagtgtggtc gacccgccga agcatataaa cacatccgga gctgtaggta
     3241 atggaggcga ttcaatgagg cggagcctcg aaaattcagt aacagcatca agatgagtga
     3301 agggattacc cccgtcccac ggattgcagg gtccccagtt gcgaagagtg acatcaaaaa
     3361 cgtcattggc cttatcattc acaaaatctc ccgtgtgata aatagggtaa gtttgcgcca
     3421 tcggggtgat cagtagcctt gtaaagaaat cccactcata ttggattaca ggaccttcaa
     3481 aaaaacctga aggcgttgta taaccgccgg gattaggtgt cccgtcgggg ttcacatacc
     3541 aggtaccagt tatcgctaca ccatcattat cagttacctg aaccatggtt ccgtttacat
     3601 caataaagac attgggaatc ccggcggtgg tatgagtacc atagacccac tgaacatgac
     3661 ggtcataaat gttcggaagg tccggttcta cagggagctg acaatcaaaa accgttgcat
     3721 cctcaaaaat aaatgcagta atatcatctc ctacacagaa cagtgttctt gggtcatcta
     3781 ccggagggtc aatgccaaga tttccggttc cctgaggtgg ggttccgtta tcccttttat
     3841 agtttggcag ctgtataacg acctgattga ctgttacata cagattgggt tgaagtgtgc
     3901 cggtattata cagataagga taaatcccga catcccaggt acagttgctg gtattgtcag
     3961 gatatccggc atatgcttca accgacctga aaattcctga tccggttccc ccaacctcgg
     4021 tatagaaggc gagggtacgg ggaaatgcat tcggatattc ccagagtatc catattttgg
     4081 gcagggcata atgtatacta ttgcgataac gcctgctcat tataatattt gactcaacct
     4141 gcacatcaag aacaggtgaa caaatgtcac ttggcggaag ctctccgtag aggccgtttg
     4201 taaaatcagc agctattgat atgtaattgt ttactgcctg gacatttgtg aaatttggtt
     4261 ccaccccacc tgttagagac atactgccaa cagcaggatt ataggataca agaacactgt
     4321 tcctgtttgc ataggttatg gatgaaggaa gtgaaatcct cagaaaatta cctgaaggaa
     4381 ccggattgcc aaccattaca acatttgcac ccccgacagt aattgtccag cctgcagaag
     4441 ttccggcagc agttaccgcc cggtcaaact caataatgag ggtttgctgc atctcatctg
     4501 taaatgtgat cggatatttg ttgccagcag cattgaccgg aatcaggtat gactgacctg
     4561 tcactacatt cagggaaagt agaccgagaa taaaaattaa cagaattcga gtactgaact
     4621 ttccaaatga tttcttattt gcttgataac cttctatttc catggtggag ctcttttctt
     4681 aacaatgcag gttcaaatat tctttcttca agacattaaa aacatacaaa agggttgcat
     4741 catctgctga tgtaacccaa taattcaaca agtagctatt tagaaacgaa ataatccccg
     4801 gaaataatat atcagcgatg atatatttta tcaaaaatca atgaattgag gaaataatca
     4861 ttcgaaatat ttagtgtttc gaaaaaaagc cttgaccggg ttttttaaat tctgcaatat
     4921 cagctcagaa aaccactcca aaatgtcatc actccatctt tgcggatggg tgtttataat
     4981 cactcctgac gggagaacac gggagagtgc catttcaatt atctcatctg ttttccttat
     5041 tctgaacttt cccctgagct gaaggccttt tgcagtcatg tccataagac ttcccctgac
     5101 cggcctgacc ttccagtcat cataactgcc gatggccatt ctttcacccg ccgtgagtcc
     5161 cctgtcacgc atgactgacc tgtcactatt ccaacttctt ccagtatcag tcagatagag
     5221 aaccgctgac acatccacat caaaataagg ttcgcaaaca attccttcag agcggtaatc
     5281 atagtacttc cacaacaacc tgttgtcaac ggcagatagc ggactgccat gcattgaaat
     5341 caccctgatc ggcgcaattc ctcttaaatc cgctagattg atccgaaacc tgctgaaagc
     5401 agcttcaacc aatgacagat caacaaatcc tcttacggca gctctccgcc ccgcaacaac
     5461 cgaaagatct tcataatgat aagcaatttc gtgacctaaa gctgcaatcg ccgatattgt
     5521 ctgataaggc agaggacctc cgggtgccct gaaatgataa cttgccctta tcccctcatc
     5581 agcctctgcc ctggccatat caagggcatt ctccggcctt ttgtcaacat catgtcttaa
     5641 aacaacgaaa cttttgtaac catcttccgg gagatcactg aagccagtga acggaatgct
     5701 gcatcctttc agcgctgtaa gaagtttccg atatttcctt agcgtaaagt ccatctgttg
     5761 caacagctaa accagattct ctttcctgtg atgattatgc ccgcaagacg ggtaattctc
     5821 aatgaaactg acgagaaaag cagtaggatc aatcatgtcg gaaaggaacc gctccctctt
     5881 cttaatccaa cgttgcttca ggtcagccat cccaagaagt tcctctgcct ttttcagcat
     5941 cagaataaac tcctcgggct gaaaggcata acaaagatca tatttgtctt ccagcatatt
     6001 tggaactgaa agcttatgtg cgaatgaatt gcatttcaaa gccggggtcc ccagtatagc
     6061 tgcttcagat gtcattgtct ggctgtcacc aacaaacatt gtcgcgaaac ttaaaaggtg
     6121 atgtattagt tccggagcta tgcgcaactg ccatttttcc agctcaggct cgatggtgcg
     6181 ttccgtggtg atataaacat ttccgtaatg ctcgagcatc ctgacaaacc ggagtttctg
     6241 ttccagggtg atccatcttt ctcctttgtc atgataggct ttccctgaaa cgaacctcgc
     6301 aatgaaataa ggttttgata aatcaattcc tgccttttca gctactgacg gatcaggggt
     6361 gaatcgtgaa ggatggagat aaaagagctc gtgagtacct gcgtaaacaa catcacggcg
     6421 gtgtttcctc tggtgagcca gtgcggaggg cgaaagtact gtgtcagcaa aaggatgggc
     6481 aaaaagggaa aaaagcctga cagcagctgc atcatcatcg tcaagcaaaa tcgatttcat
     6541 cctgtgaaag agcgatgcgt gagcaatcgt gatcgaagat ccgacagcaa tgtcaatttt
     6601 cctgtcctgc gctatcttcc ataacctgta gttgtacctt gcctgcgaaa agcccttgag
     6661 gagaagggaa tcatacttgc gccccagggg tagccatgat ataccatagg catccagaag
     6721 cttttgagca ttgggaatct cctttactgt gacagtcact tcatgccccc tggatttaag
     6781 gatcgagatt gcatttctca gcagatgaac ctgcccggga tgccctatat caaaaagcag
     6841 attcatttcc tgccactatt gtcatgattt cctgaaagct ttgctatcac tgttctcccg
     6901 gcatacacct gctcgccttc cctgaccagt atctcactgt tgcgcggaag tatcatatcg
     6961 agctgtgaac cccacctgat cttgcctatt atttctcccc tcttcaccgg atcaccgaca
     7021 tttgcagtca caatacatct cctcacaccc ctggcagcaa tttgcactat cccggtcttt
     7081 attccatcag ccctttcaat aattaccgta ttgcgctcat tcgttaccgt agaagaggga
     7141 gtgttaagcc ctatggccgt acccggcgta tgcttaatga atgtaatctt cccgtcaagg
     7201 ggtgagcggt tcatgtgcac atcaaaaagt gtcattgcaa tcccgataag ataacatggc
     7261 ctttccagga gatctgtttt ggtaatctca ctcagttcag caatccgcat cttcttaacg
     7321 gtcacaggca tctgattgac ctccagctct ttgatatata tcaccctacc gtcggcagct
     7381 gataccagat catccggccc accaggaata atcctcgacg gatttctgaa aaaccggtag
     7441 aaaaagagta acacaaaaag gataagtatt atgaacccac tgacaaatgg aaccaccacc
     7501 caaaatggaa tgggtagaat ccggtgaacc aggatggtga gaatggatcc ggccgatccc
     7561 agtaaaaggt tgtcagtata aaggtacttc ttgttgaagc ccccctttgc cacccagaaa
     7621 acaaagatag cggatgacaa caggaagcat gcgagaatta ttattatctg ttccattata
     7681 agtaaagtaa tttaccttct tttacgtgca atataaaacg aaagcaacac taaggcaaag
     7741 gaagggataa tatcacgcca ggaaaaaacc gctggtatac gctttccttt aaaggatgta
     7801 aaatactctc cgggcttcat cctgcctgag aacattgccc caaggctgaa ccaagtatca
     7861 gtcaggtagt taatccattt tacacctttt acataatcat ccggaaaagt gttctccaat
     7921 ccattgacgt acctgtaagc catgagggca taatccactc cgcatgcctt tgcaagtcca
     7981 acccacagcc aggtgcgggc attaatctca atcagcttat aattattgtc tgtaggatcc
     8041 ctgatatatt caacctcaca tattccgcag taattgagct ttctgataag cctggttgtt
     8101 gattcaagac actcctcgca ctcaatgctc tccgcatatg tggctgtacc aaatcgccat
     8161 ggatgttccc tcagcttcct cccggcccac cagcaacgaa tctcgccatc ggtacagaaa
     8221 gccgtgaatg agacagtccg gtttgtgcca tcaaatgaga tcaccgtctg agtcaaggtt
     8281 ttatccggtc caattaccct gtcaatatgg ctcaattgtt cccgtagttc ttctgtcgtc
     8341 tctgccatca gggcctttgc cttcatggcc ttgaagaatg acagcccgaa ccggcccctg
     8401 gttatcaccg ggaacttgag atcagcaggg attttctcat ctgtacggtt aaaataaaac
     8461 gtggttggta ttgcaacacc caattcagtg gccatatcaa gtagattctt cttattgtaa
     8521 atcaggtcaa tgacatccgt tcctggtgtt atcaacttga atactgaacc caacgcatcc
     8581 ctgttccttg caatcgtata gactgcatga tcattcgaag ggagcagcaa ccatccttcc
     8641 aacccctccc tgcgggcaag gtcaagaaga aaaagtgcca gatcatctga gtcaaacggg
     8701 ggtgaataaa aaaacccggt gcaataccgg gagtaccttg
//
LOCUS       DAOPBK010000110         8552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7706_length_8552_cov_4.638814, whole genome shotgun sequence.
ACCESSION   DAOPBK010000110 DAOPBK010000000
VERSION     DAOPBK010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7706_length_8552_cov_4.638814"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLA17_07405"
     CDS             <1..598
                     /locus_tag="PLA17_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPK85280.1"
                     /translation="RTVGFVANQPLEMAGVLDVDSSDKAARFIRYCDAFSIPLVTFVD
                     LPGYLPGVDQEHAGVIRHGAKVLYAYSEATVPKLTVILRKAYGGGYIAMSSRHLRADF
                     VFAWPGAEIAVMGPEGAANIIFRREIASADDPGEMRRQKVEEYREKFANPYVAASRGY
                     VDAVIEPSETRRLLLHGLEVSENKDVHRPWKKHGIPPF"
     gene            622..936
                     /locus_tag="PLA17_07410"
     CDS             622..936
                     /locus_tag="PLA17_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPK85281.1"
                     /translation="MEKKEGDRLIIDHTSYSTRLSRRFTSRKPYEPPVAGRIQSFIPG
                     TVVELLVKTGDTVKEGDDVVILDAMKMKNRLKSHIEGKVVAVNVSSGDRVAKGAVLVE
                     IA"
     gene            complement(995..1816)
                     /gene="ispE"
                     /locus_tag="PLA17_07415"
     CDS             complement(995..1816)
                     /gene="ispE"
                     /locus_tag="PLA17_07415"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPK85282.1"
                     /translation="MVVFPKAKINIGLRIMEKRPDGYHNIETFFYPVDLADALEFVVR
                     KDNRRSDSLKVTGLMKESDPENNLVMKAVKIMRQSFDFPGIRIHLHKSIPVGAGLGGG
                     SSDAAGMLKALNRYFGFGLNSEELRSFAGLVGSDAPFFIDSTPSFAEGVGNILSEFPL
                     NLSRYRIVILYPGTNISTAEAYAGCVPCPTGLSLRQLLKLPVHEWRGRVVNDFELNIF
                     RSYPGVGRLKVALYEAGALYASMSGSGSAVYGIFDGEPELPGELENYRLTTAVPE"
     gene            2031..3554
                     /gene="dnaB"
                     /locus_tag="PLA17_07420"
     CDS             2031..3554
                     /gene="dnaB"
                     /locus_tag="PLA17_07420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPK85283.1"
                     /translation="MAQGTSRGRQDKKQSVVIIPEGGRIPPQATEMEEAVLGAAMLEI
                     DAARMVVDMLRPEVFYPASHQKIFAAIVELNKRDQAIDLLTVTQELKRRGELDEAGGA
                     VYLTELTSKVVSAANIEYHARIVLQKYIQRELIRATTMIQNMVYEDTLDVTELLDKSE
                     DELFRIAEGNIKHEVTPIHEVIKQTLAEIEELGKKEDALVGTPSGFTKLDRLTSGWQK
                     SELVIIAARPSMGKTAFALTMARNMAINHGRKVAIFSLEMSAIQLVNRLIVSETEIPS
                     DKIKNGNLSPEEWSLLESRIKSLTEAPIYIDDTPAISVFELRAKCRRLKAQGRLDVVI
                     VDYLQLMTGPPDAGSREQEVSSISRSLKSIAKELTVPVLALSQLNRSVETRGGNKKPM
                     LSDLRESGAIEQDADMVVFIHRPEKMGVFQFEDGQSTRGHADIILAKNRNGPTDEVLL
                     YFREERATFIEPDDFGPEVAFTDIPSGGIFTMGSKMNDDISSDYSDFSPDDNGRRPF"
     gene            complement(3741..6491)
                     /locus_tag="PLA17_07425"
     CDS             complement(3741..6491)
                     /locus_tag="PLA17_07425"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPK85284.1"
                     /translation="MNTGPVQGTGILVLIAFLLFPAVSINAQVLGGRITDSSGAPMPY
                     ASVYITELRQGTTSNNEGWYEISLPPGNYTVNYQFLGYIPVTKSITITDEDVSASIIL
                     AEQLFEIPAVRVSASGKDPAYFIMRKAIGMAPYHLNQVKMWKAEVYIRGGGSVDRIPE
                     MLKRRMKIDANGDDLKVGKYYFTESVNMITFTAPDKYEHRVISANSSEDLTQGQVSPM
                     DFIEASFYQPVLVEMAISPLAPNAFSHYNFRFLGSSSQGNHVIDKISVTPRRKSQQLF
                     TGVIYIVEDLWAIHSLDLTNDNMAGRIRVRQLYTPVEEGIWMPVSHEFNVDISLMGIK
                     ARAAYTSAVKYLDVEPDRMLRRPAVYETAVAAGAEKEPAERPENSERPENSERPETSA
                     DREIEALLAKDNLTAREMSRLARLNRKNVSATREKPPLEIENKTTKIVDDDATSRDSA
                     YWAEVRPIPLTREESASLATAPAAKGKLAVRDTSTVTITIGRRSQPGPASAVGSDSAG
                     MKKNQTSQVIRDLTGGKRWQLSKNSSLKFDGLADLKRFSYNTVDGFVAGTGMSLSARS
                     GKAGRFTIAPSARYAFSREKLMWNVTASLLYDPMHKGNIYIRAGSQSDEFSPSGVNYL
                     VNTVSSLFFRDNIMRLYGSRYIIGGHRIDVVNGLTLDIRGKYEKRDLLTNNSSFSLFR
                     REKSWSENIPDNPYASAAGGYDYEPPVSHRHLSLESSLTWTPRQKYRISDGAKVSAGS
                     DYPTFTLTWRHGYSYNDTLSGSYDLFAAEINHTDMFGAMNQFRWRIRAGSFLNSENVR
                     LQDMHFFNTQSSPVLLNFYEDAFYLKPCYSVSARKAFAEAHLRYTTPTLLLKRLPGLS
                     RTLIRENIGISALWTPEAGYYYEAGYSLSEVFLIGTVGVYAGFTGTTFESAGIRVVLR
                     VD"
     gene            complement(6545..7243)
                     /gene="rsmI"
                     /locus_tag="PLA17_07430"
     CDS             complement(6545..7243)
                     /gene="rsmI"
                     /locus_tag="PLA17_07430"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPK85285.1"
                     /translation="MSRLILVPTPIGNLGDITIRALEVLRSAGLVLAEDTRTTRILLD
                     HYGISVPLRSHHKFNEHRSLEAVCDAIEGGTVTALVSDAGTPGISDPGFLLVRRCLER
                     EIPVESLPGPTAFVPALVCSGLPSDRFCFEGFLPQKKGRQKRISALAGEERTMVFYES
                     PFRIVKTLEQLAAALGEDRRASVSREITKKFEETIRGTLASLADHFRTTAPRGEFVIV
                     VAGSSLKDHSEESE"
     gene            complement(7236..8183)
                     /locus_tag="PLA17_07435"
     CDS             complement(7236..8183)
                     /locus_tag="PLA17_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85286.1"
                     /translation="MDFLTAVNIIGKDLEEALTSLEQLSGISGSDKAEIELARSRIGS
                     AASLVRLLPSLSGAPDEKEAVTGTAEAAPARETAPKADPAEDTIRKVASAENATRKVS
                     QAVTGAEVTYPEEEATQKVTPAEEAASKAAPEEEATRKVTAVVTKAAATDPAEETTQK
                     VSQAEAKTAVTDQAEAGTAATDPSEARATETKPVKGEPKSPEHSKAILADRFTSGGAL
                     VDKMQTEKKEEVISSVMKSKPVSDIAAAIGINDRFYFIRELFSGDALAYNDTIKRLNA
                     AASLGEAMRILDESTVMGSDPAAQSSFVDVVRRKFSLHV"
     gene            8300..>8552
                     /locus_tag="PLA17_07440"
     CDS             8300..>8552
                     /locus_tag="PLA17_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPK85287.1"
                     /translation="MEFLENSVDHAGKRGSVEVIVGSMFSGKTEELIRRLRRAMFAGL
                     KVEIFKPAVDMRYSEDKVVSHDEKSIMSTPVENASSILLL"
BASE COUNT         1907 a         2157 c         2342 g         2146 t
ORIGIN      
        1 ccgtaccgtg ggttttgtag ccaaccagcc gctggagatg gccggcgttc ttgacgttga
       61 ttcatctgac aaggcagcga ggttcatcag gtactgtgat gccttcagta tcccgctggt
      121 gacctttgtt gacctgcccg ggtatcttcc cggtgttgac caggagcatg ccggcgtcat
      181 ccgtcatggt gccaaggtgc tttacgccta cagcgaagcc actgttccca agcttacggt
      241 gatactgcgc aaggcttatg gcggtggtta catagccatg agctcgcgcc atctgcgcgc
      301 cgacttcgtc tttgcatggc ccggtgccga aatagctgtg atgggcccgg agggagctgc
      361 caacatcatc ttccgcaggg agatcgcttc tgctgacgat cccggtgaga tgcgccgcca
      421 gaaagttgag gagtaccgag agaagttcgc caatccttat gtggccgcct cccgtggata
      481 tgttgatgcg gttattgagc cgtcagagac ccgccggcta ctgctgcacg ggcttgaggt
      541 ttctgagaac aaggatgttc acaggccctg gaagaagcat ggtatacctc cgttctgatt
      601 aatacctgaa aagagagtgc aatggaaaag aaagagggag acagactaat aatagaccac
      661 acctcataca gcaccaggct gagcaggcgg tttacttcac gcaagcccta tgagccgccc
      721 gtggcgggaa ggatacaaag cttcatcccg ggaacggtgg ttgaactgct ggtcaagacc
      781 ggggataccg tgaaagaggg tgatgatgta gtcatcctcg atgcaatgaa gatgaagaac
      841 aggctcaaga gccacattga ggggaaggtt gtggcggtga acgtcagtag cggtgacagg
      901 gtggcaaagg gcgcggtgct ggtggagata gcctgattca gggagagacc ggcggagtca
      961 acccggcgga gtcagtcctg ttccggcatg agtcttactc aggtactgct gtggtgagcc
     1021 tgtagttctc cagttcgccg ggcagttccg gctcgccgtc gaagataccg taaactgcgg
     1081 atccgcttcc cgacatcgat gcatatagtg cccctgcctc gtagagtgcc actttcagtc
     1141 tgcccacccc gggatatgac ctgaagatat tgagttcgaa atcattcacc acccttcccc
     1201 tccactcatg gacgggcagc ttgaggagtt gtctgagtga gagccccgta ggacagggca
     1261 cacatcccgc ataggcctct gccgtgctga tattcgtgcc gggatagagt atcactatcc
     1321 ggtagcggct caggttaagc gggaactctg agagaatatt acccaccccc tcggcaaatg
     1381 atggtgtact gtcaatgaag aacggggcgt cactgcctac aagacctgca aaggagcgaa
     1441 gttcctcgga gttaaggccg aagccgaaat acctgttcag cgctttcagc ataccggcgg
     1501 catcggatga tccgccaccg aggcccgcac ctacgggaat gctcttgtga aggtggatgc
     1561 ggatgccggg aaaatcaaac gactggcgca tgatcttcac tgccttcatc accaggttgt
     1621 tctccggatc actctccttc atcaatccgg ttaccttcag cgagtcactc ctgcgattgt
     1681 ccttcctgac cacaaactca agagcatcag caagatcaac agggtagaag aaggtctcga
     1741 tgttatgata gccgtcaggc ctcttttcca tgatccttaa ccctatattt atcttcgctt
     1801 ttggaaatac aaccatatcc ggtctctctt gagagatcaa aaatatacga accttttcaa
     1861 tatacctcat ctttaacgat caaatccttt ttcaacatct tataaacagt tgatgttgga
     1921 aagaattagc cggacatatc cccggagacg gatgttattg ttaaatttga ttccggccgc
     1981 agtaaatgct gtaaaagttt taactaacat ataatcaatc atatatatta atggcacagg
     2041 gtacatcaag aggcaggcag gataaaaagc agtcggtggt aataattccg gagggaggga
     2101 gaatcccgcc gcaggcgaca gagatggaag aggctgttct gggtgctgcc atgcttgaga
     2161 tagatgctgc caggatggta gttgacatgc tccgtccgga ggtgttttat ccggcttcac
     2221 accagaaaat ttttgcggca attgttgagc tcaacaagcg tgaccaggca attgacctgc
     2281 tgacggtgac gcaggaactg aagagaagag gtgagcttga tgaagccgga ggggcggttt
     2341 atctcacgga gctgacatca aaggttgtct ctgctgccaa tattgaatac catgcaagga
     2401 tcgttctgca gaagtatatc cagcgtgagc tgatacgtgc gactacaatg attcagaaca
     2461 tggtctacga ggataccctt gacgttaccg agttgcttga caagagtgaa gatgaactct
     2521 tccgcattgc cgaggggaac atcaagcatg aggttacccc tatacacgag gtaatcaagc
     2581 agactcttgc agagattgag gaactgggca aaaaggagga tgctcttgtg ggcactcctt
     2641 cagggttcac gaaacttgac aggctgacgt cgggatggca gaagtcggag ctggtgatta
     2701 ttgctgcgag gccatcgatg ggaaagactg cctttgctct cactatggcc cgtaacatgg
     2761 caataaatca cggaaggaag gttgccatct tctcccttga gatgtcagca attcagcttg
     2821 tgaaccggct tattgtctct gagactgaga taccctcgga taagatcaag aacggcaatc
     2881 tctcgccgga ggagtggtca ttgcttgagt cacgtattaa gtctctcacc gaggcgccga
     2941 tttatattga tgacaccccg gccatctccg tttttgagct cagggcaaag tgccgaaggc
     3001 tgaaggccca ggggaggctt gatgttgtaa tagttgacta tctgcagctt atgaccgggc
     3061 cacccgatgc aggcagccgt gaacaggagg taagctccat ctccaggtcg ctgaagagca
     3121 ttgcgaaaga gcttaccgtg ccggtactgg ccctctcgca gcttaacagg tcagttgaga
     3181 cacgtggagg aaacaagaag cctatgctgt cagaccttcg tgaatcggga gccatcgagc
     3241 aggatgccga tatggtggtc ttcatccacc gtcctgagaa gatgggtgtc tttcagtttg
     3301 aggatggcca gagtaccagg gggcatgctg atatcatact ggcaaagaac cgtaacggcc
     3361 ccaccgacga ggttctgttg tatttcaggg aagaacgcgc cacttttatt gaacctgatg
     3421 atttcggacc tgaagttgcg tttactgaca ttccttccgg aggtatcttc acgatggggt
     3481 caaagatgaa tgatgatatt tcatctgact acagtgattt ctctcctgat gacaacggtc
     3541 ggagaccgtt ctgagtcagt cagtggcaat atgccggtat gagggggatg atgctgagtg
     3601 ccctatgatc atcatgctga tgtactacgc tgaataacaa tgttttgtct cttgcgcaaa
     3661 atgaattgcc tggcattctg tactgcagta cccgattgtc cggagcggac agatggccat
     3721 cagccattgt aaacccggtt ctaatcaact ctcaatacca ccctgattcc ggctgactcg
     3781 aatgttgtac ctgtgaatcc ggcataaacg ccaaccgtgc cgataagaaa cacctcgctg
     3841 agggagtaac ctgcctcata gtaatatccg gcctcaggtg tccacaacgc tgatattcct
     3901 atattctccc tgatgagtgt acgactcagg ccgggcaggc gcttgagcag gagggtgggg
     3961 gtggtgtacc ttaaatgtgc ctcggcaaat gccttccttg cagagacgga ataacatggc
     4021 ttcagataaa atgcatcttc gtagaaattg agcagtaccg gtgacgattg tgtattgaag
     4081 aagtgcatgt cctgcagcct gacattttca ctgttgagga agctaccggc cctgatccgc
     4141 cagcgaaact gattcattgc cccgaacatg tctgtgtggt ttatctccgc tgcaaatagg
     4201 tcatagctgc ctgaaagggt gtcattgtag ctgtaaccgt gtctccatgt cagggtaaag
     4261 gtcgggtaat cagatccggc actcaccttt gcgccgtcag aaatacgata cttctgccgt
     4321 ggtgtccatg tcagggatga ctcaagggag agatgcctgt gactcaccgg cggctcatag
     4381 tcgtatcctc cggctgctga agcatacggg ttgtcaggta tgttctcaga ccaggacttt
     4441 tccctcctga agagggagaa ggaggaatta tttgtcagca gatcacgctt ttcgtacttc
     4501 ccccgtatgt caagcgtcag cccgttgaca acgtctatgc ggtgtccgcc tatgatgtac
     4561 cggcttccgt agagtcgcat gatgttatcc ctgaagaaaa gggatgatac ggtattaacc
     4621 agataattaa cacctgacgg agagaattcg tcagactggc tgcctgccct tatgtaaata
     4681 ttccctttgt gcatcgggtc atagagcagg ctggcagtga cattccacat cagtttttcc
     4741 ctgctgaatg cataccttgc cgaaggggca atggtgaagc gtcctgcttt gcctgatctg
     4801 gccgacaggc tcattcccgt tcctgccaca aaaccgtcaa cggtattgta ggagaatctc
     4861 ttcaggtctg ccaggccgtc aaatttaagg gagctgtttt ttgagagctg ccatctcttt
     4921 cctcctgtca ggtctctgat gacctggctt gtctggtttt ttttcatgcc cgcggagtcg
     4981 gagccgacgg ctgaagccgg tccgggctgt gatcttctgc cgatagtgat ggtcactgtt
     5041 gaggtatcac gcactgccag ctttcctttt gcagccgggg ctgttgccag gctggcgctc
     5101 tcttccctgg tcagcgggat gggccgcacc tcagcccagt atgctgaatc acggctggtg
     5161 gcatcatcat caactatctt cgtggttttg ttttctatct ccaggggagg cttctcacgc
     5221 gtggcgctga catttttcct gttcagccgc gccagccgtg acatctcccg tgccgtaagg
     5281 ttatccttgg ccagcagagc ctcaatctca cgatcggcag aagtttcagg ccgttcagag
     5341 ttttcaggcc gttcagagtt ttcaggccgt tcagcaggct ctttctcagc cccggctgcg
     5401 acagcggttt catatacggc tggccgccgg agcatcctgt caggctccac atcaaggtat
     5461 ttgactgcgc tggtgtacgc agccctggcc tttattccca tgagtgaaat gtcaacattg
     5521 aactcatggc tgacgggcat ccagataccc tcttccaccg gtgtgtaaag ctgccgtacc
     5581 cttatgcgtc cggccatgtt gtcattggta agatcaaggg agtgaatggc ccacaggtcc
     5641 tccacgatgt atatcactcc ggtgaagagc tgctggctct tccggcgcgg tgtaacgctg
     5701 atcttgtcaa tcacatggtt gccctgcgat gatgaaccaa gaaatctgaa gttataatga
     5761 gagaatgcat ttggcgcaag gggcgatatt gccatctcta ccagtacagg ctgatagaaa
     5821 ctggcttcga tgaagtccat cggtgagacc tgcccctgtg tcagatcttc gcttgagttg
     5881 gcggagataa cccggtgttc gtatttgtca ggagctgtga atgttatcat gttcaccgac
     5941 tcagtgaagt agtatttgcc caccttcagg tcatcgccgt ttgcgtcgat cttcatcctc
     6001 cgtttgagca tttcaggaat ccggtcaaca ctgccaccgc ccctgatgta gacctcggct
     6061 ttccacatct taacctggtt gaggtgataa ggcgccatac ctattgcctt gcgcatgatg
     6121 aaatatgcag gatcctttcc tgatgccgag accctcaccg ccggtatctc gaagagctgc
     6181 tctgcaagga ttatactggc agagacatct tcgtcggtga tggttatgct ttttgtcacc
     6241 ggaatgtatc cgaggaactg gtagtttacg gtgtagttgc cgggagggag ggatatctcg
     6301 taccagccct cgttgtttga ggtggtaccc tggcgcagtt cagtgatata gacagaggcg
     6361 taaggcattg gcgctccgct gctgtcggtt atcctgccgc ccaatacctg ggcattgatt
     6421 gaaacggcag ggaagagaag aaatgcaata aggaccagta tccctgtacc ctgaacaggg
     6481 ccggtattca taatgttcgg aaaggaagaa ggtgcctccc ggatttgtct cctgccgcgc
     6541 atctttattc tgattcttcg gaatggtctt tcagtgagga gccggccacc acaattacaa
     6601 attcgcccct gggggcggtt gtgcggaagt gatctgccag tgaggcaagg gtcccgcgaa
     6661 tggtctcctc gaattttttc gttatctcgc gtgagacgga agcccttcgg tcttcaccaa
     6721 gggcagcggc aagctgttcg agggttttga caatacggaa gggtgattca tagaaaacca
     6781 ttgtgcgctc ttccccggcc agggccgaaa tccttttctg tctccccttt ttctgtggaa
     6841 ggaatccctc gaaacagaag cggtctgatg gcaggcctga gcataccagt gcaggcacga
     6901 aggcggtggg gccgggaagt gactccacgg gtatctctct ctcgaggcac ctgcgaacca
     6961 ggaggaagcc cgggtcggag ataccggggg tgccggcgtc agatacaaga gctgtcaccg
     7021 tgcctccctc aatggcgtca cacaccgcct cgaggctgcg atgctcattg aatttgtggt
     7081 gcgaacggag cgggacactt atcccatagt ggtcaagaag gatccttgtg gtcctggtgt
     7141 cttcggcaag gacaaggccg gccgatctca gcacctccag ggcacggatg gttatatcgc
     7201 caaggttgcc gatgggtgtg ggcaccagga taagtctaga catgcaggct gaattttctg
     7261 cgtaccacgt caacgaatga tgactgagcg gcaggatcgc tgcccatgac ggtgctttcg
     7321 tcgagtattc tcatcgcctc gcccagtgac gctgccgcgt tcagtcgttt gatggtgtca
     7381 ttgtatgcca gcgcatcacc gctgaagagc tcacggatga agtagaacct gtcattaatg
     7441 cctatggcag cagctatatc ggagacaggc ttgcttttca tgactgaaga gatgacctcc
     7501 tccttctttt ccgtctgcat cttgtcaaca agggctccgc ctgaggtgaa gcggtcagca
     7561 aggattgcct ttgaatgctc cggactcttt ggctctccct tcaccggctt cgtttcagta
     7621 gcccgggctt cagacgggtc tgtcgcggca gtaccggctt cagcctggtc tgtcacggca
     7681 gttttggctt cagcctgact gaccttttgg gtggtctctt cagccgggtc tgtcgcagca
     7741 gctttggtta ctaccgcagt gaccttccgg gtggcctctt cttccggcgc tgcctttgaa
     7801 gcggcctctt cagccggagt gaccttttga gtggcctctt cttccggata tgtcacctcg
     7861 gctccggtca cagcctgact gacctttcgg gtagcattct cagctgaggc gactttccgg
     7921 attgtgtctt cagccgggtc tgcctttgga gcggtctccc tggctggcgc tgcctctgca
     7981 gttccggtca cagcctcctt ttcatcaggt gccccgctga gtgaaggcag caatctgacc
     8041 aggctcgcag cgcttcctat gcgtgacctg gcaagctcta tttccgcttt atccgatcct
     8101 gatatgccgg agagctgttc cagcgaagtc aacgcctcct ccagatcctt tccaataatg
     8161 ttgacagctg tcaggaaatc catctcgctt agttttttaa ttatttccgt tttctttatt
     8221 ttgcaaaagt aatcaataat agtttttcgg gctgaataat tcaatcctga gtattgaacg
     8281 gcccggtaac aactgaagta tggagtttct cgaaaattcg gttgaccatg ccggcaagcg
     8341 gggatcggtg gaggtgattg tcgggtctat gttttccggt aagaccgagg aactgataag
     8401 gagattgcgc agggcgatgt ttgccgggct gaaggtggag attttcaagc ctgccgtaga
     8461 tatgagatac tctgaggaca aggttgtgtc acatgatgag aagtcaatca tgtctacccc
     8521 tgtggagaat gcatcctcta tacttctcct tg
//
LOCUS       DAOPBK010000111         8533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7725_length_8533_cov_4.301604, whole genome shotgun sequence.
ACCESSION   DAOPBK010000111 DAOPBK010000000
VERSION     DAOPBK010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7725_length_8533_cov_4.301604"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..703)
                     /locus_tag="PLA17_07445"
     CDS             complement(2..703)
                     /locus_tag="PLA17_07445"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPK85288.1"
                     /translation="MKKTMTLLAAFLVASVCSAQSLDEIANKYYAANGIEILEKSQTI
                     MMKGIVSQMGMELPITIMVKQPNKVKVIQEFNGMEIITLFDGEKGYMVNPLTGATEPI
                     ELPAEQLGSVQEYNVFKDSFMEAYKAGKMELEGEEEVEGKPAYKIAITNESGNTSITY
                     LDKESFLPVKTEQTVSQMGMEMLVEAYVKERKEINGVKFGTRIAQFINGSEMGDITFE
                     TIEFDTPIEDSVFQL"
     gene            complement(725..1531)
                     /gene="kdsA"
                     /locus_tag="PLA17_07450"
     CDS             complement(725..1531)
                     /gene="kdsA"
                     /locus_tag="PLA17_07450"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPK85289.1"
                     /translation="MPLLKHSCSGNFLLLAGPCVVESEEMTFRVAAEVVKLSDKYHIP
                     LIFKSSYRKANRSRLDSFSGIGDEKALGILAEVREKYSVPVITDIHTPEEAHMAAHFV
                     DVIQIPAFLCRQTDLLVAAAATGKVVNVKKGQFLSGASMRFAAEKIRESGNQRVMLTE
                     RGSFFGYHDLVVDMRNIPVMKQIGVPVIMDITHSLQQPNQEGGVSGGQPDMIETIGRC
                     AIAAGADGIFIETHPEPASALSDGANMLRLDLLDSLLNKLTLIRTTVNSL"
     gene            complement(1573..2877)
                     /locus_tag="PLA17_07455"
     CDS             complement(1573..2877)
                     /locus_tag="PLA17_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPK85290.1"
                     /translation="MFSNQPLADRLRPRSLDEFAGQEHLVGNEGVLRKVIESGNVPSF
                     ILWGPPGVGKTTLARIVANRLERSFFQLSAVHSGVKEVRETIEKAKSQQFFGKPNPIL
                     FIDEIHRFNKSQQDSLLGAVEQGILTLIGATTENPSFEVISPLLSRCQVYVLKPLDDE
                     TLRKLVDRAITEDYYLSQYKIRIAEYEALIRISGGDARKLYNALELTASLEAEKESGE
                     IVVTDEMVLGHVQQNLAMYDKLGEQHYDIISAFIKSIRGSDPNAAVYWLARMVEGGED
                     VRFIARRMVIHAAEDIGLANPNALLLAQACFNAVNLVGWPESKLILSETAIYLATSPK
                     SNSSYIAIENALAAVREHGDLPVPLHLRNAPTRLMKDLGYSEGYRYAHDFEDNFVQDN
                     FLPDQITGTVFYEPGRNARELEIRKQLEARWQKIYGYKKNNE"
     gene            3071..4231
                     /locus_tag="PLA17_07460"
     CDS             3071..4231
                     /locus_tag="PLA17_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85291.1"
                     /translation="MKKPLLITLLVLAVLLLIPAVSFIKWAFQEKKPVDVVILDKTVP
                     TLDRLGHRSFVYILTNARYVRGEKGGSYSARKDYYGFEPLRPVREKQFRKKDFRLTEL
                     IELAEKNDALYYTDTYGVFFNDWYQGIKKTRRSRKLYGGLNNNDYLLMVEMKKRDKLV
                     LLEYNTFDYPTAGLEKFKTEELLGIASKGWMGQYYKSLDTTSHQGVPAWMPALYRNQY
                     REPWTFTKPGVVLLRENGIIVLEEGKHLNSAMPVIRTTGPSAERFGLPESVAFTNSFD
                     IIDPGSNNVISEFVLNTTPSGDTLLAINDLEAVFPAVTQEPLTQRTYYFSGDFANNKI
                     PFWTARFKNIQKGAKSFLYSEKPEDPKRFFWLYYKPLITTIFEEYQAEPAGN"
     assembly_gap    4265..4364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4794..5561
                     /locus_tag="PLA17_07465"
     CDS             4794..5561
                     /locus_tag="PLA17_07465"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPK85292.1"
                     /translation="MKATIVQCGLKWEERDANLAHISCLLDAASPGEGIVVLPEMFTT
                     GFTMNPAPLAEGMDGPTIRWMKERSANGGYALCGSVIIREEENYYNRLIFVTRDGEVT
                     FYDKRHLHTMSGEHTVYSPGNKRVVVPYCEFNFNFQICYDLRFPVWSRNRGDTDVFVY
                     VANWPLVRSYAWKSLLVARAIENQCYVIGVNRVGANPDGTSYTGDSAIIGPKGELLAS
                     LEPGTEGVVSAILSRESLDKYREDMPIWRDADSFETV"
     gene            complement(5725..>8533)
                     /locus_tag="PLA17_07470"
     CDS             complement(5725..>8533)
                     /locus_tag="PLA17_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HPK85293.1"
                     /translation="IKKLLAFAAKHRIGIIPRAAGTSLAGQVVGSGIVADLSRHFGKI
                     LEINTEERWVRLEPGVVPDELNAALRSHGLLFGPETSTSSRCNLGGMIGNNGCGLHSV
                     VYGSVRDHIIEVRGLLHDGSEVVFSELTADQFNQKCSLQTAEGEIYREVERIMTNPVH
                     RESIMAGYPEKSIPRRNTGYALDDLLYSEPFTPGADRKVNLARLICGSEGTLAIVTEA
                     KLGLVPLPPPVKALACVHLHHRNEAFRANLIALQYQPWAVELMDSTILNLAATVESQK
                     RNRFFIEGDPGALLIVEFCAGSREEIISTVAAMEREMRSRELGYAFPVVWNGDVKRVW
                     DLRKAGLGILSNMPGDARPVSLIEDCAVDVNRLGDFVAEIEEMLASYGKESVYHAHIG
                     TGELHIRPLINIKDTGEVKLLRTIAEETARIVKKYRGSMSGEHGDGRLRGEFISIIIG
                     QHNYELNKRIKRVFDPHGILNPGKITDTPPMDSSLRYTPGKPTPEPATHYDFSDCGGV
                     VRAAEKCNGSGDCRKSSAIGGMMCPSFMATGDERLTTRARANIMREMLYSERPNPWDS
                     HEMYEILDLCLACKGCRSECPSGVDVARLKSEFLQHYNDVHPPSLRTHLIASLPKISG
                     LFSFIPGIFNFFAANKLTALIIKKVTGFAVERSIPLLAPMTFRRWLKRNLPLLNPAAP
                     AGDVCLFVDEFTNHNDLDAGIATARLLTGLGYRIHIAGNVASARTYISKGFLRKAKKL
                     ITKNIQVLGPLVSDSCPLVGIEPSAILGFRDEFPDLAGDRLRPEASRLAQNAFTLEEF
                     IASEFKAGRIRRDMFTGEPAEVLVHVHCHEKAITTSAPVLAALGIPANYRVREIPSGC
                     CGMAGAFGYEKEHYELSRKVGELVLFPDVRSASPATIICAPGTSCRHHIKDGTGRRAI
                     HPAEVLFRAMRPGWQAVQKAD"
BASE COUNT         2045 a         2194 c         2044 g         2150 t
ORIGIN      
        1 attacaattg gaagaccgaa tcctcaatcg gtgtgtcaaa ctctatcgtt tcgaaggtta
       61 tgtctcccat ctctgatccg tttataaact gagcgatcct ggtcccgaat ttgacgccgt
      121 tgatctcttt tctttccttt acataggcct cgaccagcat ttccattccc atctggctga
      181 ccgtctgttc ggtcttgaca ggcaggaagc tctccttgtc gaggtatgtg atcgatgtgt
      241 tgcctgattc attggtaata gctatcttat aggctggttt gccttccacc tcttcctcac
      301 cttcaagttc catcttcccg gccttgtaag cttccatgaa agagtctttg aacacattgt
      361 actcctgcac actgcccaat tgctcggcag gaagctcaat cggttcagtg gcaccggtga
      421 gcggattcac catgtatccc ttctcaccgt caaagagcgt gatgatctcc attccgttga
      481 actcctgaat taccttgacc ttgttgggtt gtttgaccat aattgttatt ggcaattcca
      541 ttcccatctg gctgactatg cccttcatca ttatggtctg agacttctcc agtatctcaa
      601 tcccgttggc tgcataatac ttgtttgcaa tctcatcgag cgactgggca ctgcagactg
      661 atgccaccag aaacgccgcc agaagagtca ttgttttttt cataataact ttaattgttg
      721 tttattataa tgagttaact gtagttctta ttaaggtgag tttattaagc aggctgtcga
      781 gcaaatcgag tctcagcatg ttggctccgt cagagagtgc agaggccggt tccgggtggg
      841 tttcgatgaa gattccgtca gcgccggctg caatggcaca cctgccgatg gtctcaatca
      901 tgtcaggctg tccgcctgat actccgcctt cctggttcgg ctgctgcagt gaatgagtga
      961 tatccattat taccggaacg ccgatctgct tcataaccgg gatgttgcgc atatccacaa
     1021 ccaggtcatg gtatccgaaa aagcttcccc tctcggtaag catcactctc tgattacccg
     1081 attcccgtat cttttccgct gcaaaacgca tcgaggcacc ggaaagaaac tgtcccttct
     1141 tgacatttac caccttcccg gtggcagctg ccgcaacaag gagatctgtc tgcctgcaca
     1201 gaaaagcagg aatctggatg acatcgacga aatgggctgc catgtgggct tcctcaggcg
     1261 tgtgaatgtc agtaatcacc ggcacggaat acttctccct gacctccgca agtatcccga
     1321 gcgctttctc gtcgcctatg cctgaaaatg aatcaagcct cgaccggttt gccttacggt
     1381 atgatgactt gaaaatcaat gggatatgat atttgtcgct gagctttacc acctcggcag
     1441 ccacacgaaa ggtcatctct tctgactcaa ccacacatgg tcctgcaagg agaagaaagt
     1501 tcccgctgca gctatgcttc aacaatggaa tattcagttc actgactttc atgagataaa
     1561 gttattaata ttttattcgt tattcttttt gtatccataa attttttgcc accgtgcctc
     1621 cagttgcttt cgtatctcaa gctcacgggc attcctgccc ggctcgtaga agaccgtgcc
     1681 ggttatctga tccggcagga aattgtcctg aacaaagttg tcctcaaaat catgggcata
     1741 cctgtacccc tcgctatatc ccagatcttt catgagcctg gtgggagcat tgcgcagatg
     1801 aagcggcacc ggcaggtcac catgctccct gacagcagct aatgcgttct ctatggctat
     1861 gtaggagctg ttgctcttgg gcgatgtagc aaggtatatt gctgtttcag agaggattag
     1921 ctttgactcg ggccacccga cgagattcac ggcgttgaag catgcctggg cgagcagcag
     1981 ggcattggga ttggcaagac cgatgtcctc ggcagcatgt attaccattc gccgggctat
     2041 gaacctgaca tcctcaccgc cttccaccat ccttgcaagc cagtataccg cggcattggg
     2101 gtctgacccg cggatagact tgataaatgc tgaaataatg tcataatgct gctctccgag
     2161 cttgtcatac attgcaaggt tctgctgcac atggccaagc accatttcat cagtgacaac
     2221 aatctccccg ctctccttct ccgcctcgag cgaggcagtc agttcgagcg cattgtacag
     2281 ttttcgtgca tcacctccgc tgatgcgaat cagcgcttca tattcagcga tcctgatttt
     2341 gtattgagag aggtagtaat cttctgtgat tgccctgtca accagcttcc tgagcgtttc
     2401 gtcatcaaga ggtttaagca catacacctg gcaccttgag aggagcgggg agataacctc
     2461 gaaggaggga ttctctgttg ttgcaccgat cagtgtcaga atcccctgct ccacggcacc
     2521 caggagtgag tcctgctgcg acttgttgaa gcgatgaatc tcgtcgatga aaagtatggg
     2581 attcggcttg ccgaaaaact gctggctctt tgccttttca atggtttcgc ggacctcctt
     2641 caccccgctg tgtactgcac tcagctggaa gaatgaacgc tccagcctgt tggcaacaat
     2701 ccttgccagt gtggtcttcc ccacaccggg agggccccat aatatgaatg acggtacatt
     2761 gccggactca atcaccttcc tcagcacacc ctcattaccc acaagatgct cctgcccggc
     2821 aaactcatcg agtgaccgtg gtcttaatct gtcagcaagt ggctgatttg aaaacatatg
     2881 gcaatttcat taacagaagt gcaaattatc acaaattcgc ttgaaccgtt acactttttg
     2941 ccaaattccg ataaaatatt ttcccggata tgaaataatt tggtacttta gcaattaatt
     3001 ttacacgaca cgtatactgt atccaccaag aattcgttta gtaacaggat attatcaaat
     3061 tccggtcatt atgaaaaaac ctctactgat tacattgtta gttctggcag tattgctctt
     3121 aataccggct gtgagcttca tcaaatgggc tttccaggaa aaaaagcctg tagatgttgt
     3181 catacttgac aagactgtcc cgacacttga cagacttggc caccgttcat ttgtctacat
     3241 cctgactaat gcaagatatg taaggggaga aaagggcggc agctattcgg caagaaagga
     3301 ctattacggc ttcgaaccgc tcagacccgt gagagagaaa cagttcagga aaaaagactt
     3361 ccgcctcacc gaactgattg aactggcaga aaagaatgac gcattgtatt acactgatac
     3421 atacggtgtg ttcttcaacg actggtacca gggaataaag aagacacgcc ggtcgcgcaa
     3481 gctttatgga ggactcaaca acaacgatta tctccttatg gtggagatga aaaaacggga
     3541 taagctggtg ctccttgaat acaatacatt cgattatcct acggcaggcc ttgagaaatt
     3601 caagacagaa gaacttctcg gaattgcttc gaaaggatgg atgggacagt attacaagtc
     3661 gctcgataca acgtcacacc agggcgtgcc tgcctggatg ccggccctct accgtaatca
     3721 ataccgcgaa ccatggacat tcaccaaacc gggagtggta ctgctgaggg agaatggtat
     3781 catagtgctg gaagagggta aacatcttaa ctcagcaatg ccggtcatca gaacaacagg
     3841 gccgtcagca gaaagattcg ggcttcccga gtccgttgcc tttacaaata gcttcgatat
     3901 catagacccg ggaagtaaca acgtgatttc cgagttcgtc ctgaatacaa ccccttcggg
     3961 tgataccctg ctggcaatca atgacctcga agctgtattc cccgcagtaa ctcaggaacc
     4021 actcacacag agaacatatt acttctcagg cgatttcgca aacaataaaa tacccttctg
     4081 gacagccagg tttaaaaaca tccagaaagg agcgaagagt tttctctatt ctgaaaaacc
     4141 ggaggatccg aaaagattct tctggctgta ctataagccg ctaattacaa ctatttttga
     4201 ggaataccag gcagaacctg ccggaaatta acagacagac aaacagcaag gaagaccgtc
     4261 tcatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcaga acctgccgga
     4381 aattaacaga cagacaaaca gcaaggaaga ccgtctcatg agataattga gacggttttt
     4441 ttatttgagc ttttctgaag gttcttaatt aacagatcca ccggcatgtt tttgctttat
     4501 ggagtatccg cgtatggata ttctgctgaa tacaatgaca ctacttcaaa cctgatgccc
     4561 tgcgccatct cctcttcggt tggatattct gccggacaca atgacacagg tgctgtttta
     4621 taaaggttat tgcatatctt tatgtgcagg tatgcagcaa caacagctcg gagtattctt
     4681 cagatgattg ctaataacta cctgatcatc agaattgatc atctttctta aagtgagtgc
     4741 tctaattaat tgattatgag tctgtaagca aaaaatacct gtcttttaca gcaatgaaag
     4801 caactatagt acaatgcgga ctgaaatggg aggagaggga tgccaacctg gctcatatat
     4861 cctgcctgct tgatgcagct tctcccggag aggggattgt ggtgctgccc gagatgttca
     4921 caaccggttt tacaatgaat cctgcacctc tggcagaagg aatggacggg ccaacaataa
     4981 gatggatgaa ggagcggtca gcaaacggag gttatgctct ctgcgggtcg gtgatcataa
     5041 gagaggagga gaactattat aaccgcctga tatttgttac cagagatgga gaggtgacat
     5101 tctacgacaa gcggcatctt catacaatga gcggtgagca tactgtttac agccctggta
     5161 ataagcgggt tgttgtgcct tactgcgagt ttaactttaa ctttcagata tgctacgatc
     5221 tgcgctttcc tgtatggtca aggaaccggg gagataccga cgtatttgtc tatgtggcaa
     5281 actggcccct ggtccgcagc tacgcctgga agtcgctcct ggtagccagg gctatagaga
     5341 atcagtgtta tgtgatagga gtgaaccgtg tgggagcaaa tcctgacggt acttcataca
     5401 ccggtgattc ggccattatc ggtcccaaag gggaactgct ggcctccctt gagccaggca
     5461 ccgagggtgt tgtctctgcc attctctccc gcgaatctct tgataaatac cgcgaggata
     5521 tgcctatatg gcgcgatgcc gacagctttg agactgttta gccggcattc agttatcaat
     5581 cagtacttta gtgcaggata tgcccgcctg gcgcgatgcc gacagctttg aaactattta
     5641 gtcagcaccc gggtgctcaa tcagcacttt agtgcagaaa atgcccattt ggcgcgatgc
     5701 gggcacttag tcaaaactta ctgctcaatc agctttttgt actgcctgcc aacctggcct
     5761 catggcccgg aagagcactt ccgccggatg aatggctctc ctgcctgttc catccttgat
     5821 gtggtgacga cagctggtgc cgggggcaca gatgatggtt gccggtgagg ccgacctgac
     5881 atcgggaaaa agaaccagtt cacccacctt gcgggaaagc tcataatgct ctttttcata
     5941 cccgaatgca cctgccatcc cacagcaacc tgacgggatc tccctgaccc ggtagtttgc
     6001 cggtataccc agggcagcaa gcacaggcgc cgaagtggtg attgcctttt cgtggcagtg
     6061 gacatgtacc agcacctctg caggctcacc ggtgaacata tcacgtctta tccttcctgc
     6121 cttgaactcg ctggcaatga attcctcaag ggtaaaagcg ttctgagcca gccttgaagc
     6181 ctccgggcgt aacctgtcac cggcaagatc agggaactca tccctgaaac ccaggatagc
     6241 cgagggctcg atgccgacca gcggacagga gtcgctcacc agtggaccca aaacctgtat
     6301 atttttggtt atcagcttct ttgccttgcg gagaaatccc ttggagatat atgtacgtgc
     6361 acttgctaca ttaccggcga tgtggatacg gtacccgagg ccggtcagca acctggcagt
     6421 ggcaatgccg gcgtcgaggt cgttatggtt tgtaaattca tctacaaaga ggcagacatc
     6481 accggcagga gcggccggat taagcagagg cagattcctt ttcagccacc gtctgaaagt
     6541 catcggcgcc agcagcggta tgctccgttc aacggcaaaa cctgtcacct tcttgattat
     6601 cagtgcagtg agtttgttgg cggcaaagaa attaaagatg cccggaataa aggagaaaag
     6661 gccggagatc ttcggcagcg aagcaatcag atgtgttcgc aatgacggag ggtgcacatc
     6721 attatagtgc tgcaggaact ccgacttgag cctggccaca tccactcccg aagggcactc
     6781 gctgcggcat cccttgcagg caaggcacag gtcgaggatc tcatacatct catgactgtc
     6841 ccaggggttt ggtctttcag aataaagcat ctccctcatt atatttgccc tggcacgggt
     6901 tgtaagtcgc tcatcaccgg tggccataaa gcttgggcac atcatgccgc ctatggcgct
     6961 gctcttgcgg cagtcacccg acccgttgca tttttcggct gcacgcacca cacctccgca
     7021 gtcagagaaa tcgtaatgcg ttgccggttc cggtgtgggc tttcccggag tatagcgcag
     7081 cgagctgtcc atgggagggg tgtccgttat cttgccggga ttgagtatgc cgtgagggtc
     7141 aaaaaccctc tttatgcgct tgttgagctc atagttgtgc tgcccgatta taatggagat
     7201 gaactctccc cgcagccttc cgtcaccgtg ttcgccgctc atcgagccac ggtatttttt
     7261 cactatgcgg gcagtctctt cagctatggt gcgtaaaagc ttcacctcac cggtatcctt
     7321 gatatttatc agaggcctga tatgaagctc tccggtacct atatgtgcgt gatatacaga
     7381 ctctttgccg taagaggcca gcatctcctc tatctcggcc acgaagtcgc ccagcctgtt
     7441 cacatccaca gcgcagtctt caatgagaga gaccggcctg gcgtcgccgg gcatgtttga
     7501 cagtattccg agtcctgcct tccggagatc ccataccctc ttaacatcgc cgttccagac
     7561 cacgggaaac gcatatccca gctcccggct gcgcatctct ctctccatgg ctgccacagt
     7621 ggagattatc tcttcacggc tcccggcgca gaactccact atgagcaggg cacccggatc
     7681 accctcgatg aagaacctgt tccgtttctg gctctcgacg gtggctgcca gattgagtat
     7741 ggtgctgtcc atcagctcca cagcccatgg ctggtattgc aatgcaatca ggttggcgcg
     7801 gaaagcctcg ttacggtgat gcaggtggac gcatgcaaga gccttcaccg gcggaggcag
     7861 cggtaccaga cccagcttcg cctcagtgac aatggcaaga gtcccttccg acccgcagat
     7921 gagccttgca agattgacct tacggtctgc accgggtgtg aatggctcac tgtaaagcag
     7981 gtcatcaagt gcatatccgg tgttgcggcg gggaatgctc ttttcaggat agccggccat
     8041 gattgattcc cggtgaacgg gatttgtcat gatacgttca acttccctgt atatttcacc
     8101 ctcagcggtt tgcagtgagc acttctggtt gaactgatcc gcggtgagct cgctgaagac
     8161 aacttctgat ccgtcgtgca gcaggccacg gacttcgatt atatgatccc tgaccgagcc
     8221 gtagacaacg gagtgaagtc cgcagccgtt gttcccgatc attcctccca ggttgcaccg
     8281 gctggaggtc gaggtctccg gcccgaacag tagcccgtgg cttcgcaggg cagcattgag
     8341 ctcatccgga accaccccgg gttcgagcct gacccatcgc tcttcagtgt ttatttccag
     8401 tattttacca aagtgacgcg agagatcagc cactataccc gaacccacca cctgccctgc
     8461 cagcgaggtc cccgcagccc tcgggataat gccgatacgg tgcttcgcag caaatgcaag
     8521 aagctttttt atg
//
LOCUS       DAOPBK010000112         8374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7891_length_8374_cov_6.167328, whole genome shotgun sequence.
ACCESSION   DAOPBK010000112 DAOPBK010000000
VERSION     DAOPBK010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7891_length_8374_cov_6.167328"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1130
                     /locus_tag="PLA17_07475"
     CDS             141..1130
                     /locus_tag="PLA17_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPK85294.1"
                     /translation="MKKILGIGNALVDVMTIIPDETCLGRFSLPKGSMTMVDAHRSGE
                     IKQAINGMKRTLASGGSAGNTMYGLGIMGVNSSFIGKVGRDEMGNFYEKDMVDAGLTP
                     VLIRSSQSPTGTAVALVTPDSERTFATHLGAATELSADELNISYFKGFNILYIEGYLI
                     YNLPLAEKAFQLAKENRMCVALDLASFNVVTELLPSFDRLVREYVDIVFANEEEARAF
                     TGGLNPREALDAIAGKCKIAVVKMGPEGSWIKRGDEVIKVEALKVKAVDTTGAGDLYA
                     AGFLYGFSNGFSLDKCGVFGSILAGKVIEVVGARMPEEKWAEARKLVDTVARE"
     gene            complement(1143..1793)
                     /locus_tag="PLA17_07480"
     CDS             complement(1143..1793)
                     /locus_tag="PLA17_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPK85295.1"
                     /translation="MFRSGTFLICFTLLFVSGICSGQKTWWGEISGFAGGGGNDIFRF
                     QELVGSGSVTGKGFWTAGADLRRLLGDHFSIGTGLSYSHQYYYTSPAPGIGGTDTPGS
                     FGLVTLPVMVRADFLKWFFADAGALVAVQTGSSRVDDMSGVGATVGAGIQYRFKSDMF
                     IRVRAYVAQYGLLHFSPEDHPQTLLNGGVTLGVGYRFIRLGSCNCPDDRSPVRRFR"
     gene            complement(1804..3051)
                     /gene="fucP"
                     /locus_tag="PLA17_07485"
     CDS             complement(1804..3051)
                     /gene="fucP"
                     /locus_tag="PLA17_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HPK85296.1"
                     /translation="MTNETLSKPTRRLVTREFIVPFILVTSLFFLWGVAHSLLDVLNK
                     HFQDILGITRARSGLVQAALYGGYFIMAIPAGLFMSRVGYRKGIITGLLLYAAGAFLF
                     WPATFIRTFEFTLLCLFIIACGLTFLETAANPYSSVLGPKETSEQRLTLSQSFNGLGW
                     IIGPALGGMLILGGNGDDSANNFSSMALPYMLIGGVVLVVAFMFVITRMPEIHEGEHD
                     SGGDRGTYRGLIRVRHFRYAVVAQFLYVAAQTGINSFFINYVVETMPGYSPEKAAYLL
                     SLGFVFFMSGRFVGSLFMSWFSPRQMLGIYALINTAAMVLVMLGLGWPSLVALYLNYF
                     CMSIMFPTIFALGLKDLGAMTKKGSSVLVMTIVGGAICPVIMGRIADVSGMAIGFAVP
                     LVCFAFIAWYAFCRAKSDKIIIK"
     gene            3224..4729
                     /locus_tag="PLA17_07490"
     CDS             3224..4729
                     /locus_tag="PLA17_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPK85297.1"
                     /translation="MRTLLLTVAILLLAAFTRTAPAQQTDEQLILKHFHSISSIEIAE
                     WMAEICSPKYNGRLAGTPEYIASAEWMAGKFREWGIKPAGDNGSYFQWFDHPYTVVNE
                     PGELKLHITQKDGVTIVKNYKAPYDFYPGMNSGNGEVTAEVVWVGYGTTAPELNYDDY
                     KNVDVRGKIVLMNRDVPFKDTRDPEYAKWVKYCYHHYKAENAARHGAAGMLYIDGNSA
                     NPNISYIENMVVTGIGPEPLSDIFAGLGKNNKTLLDQVDKTFKPASFATGKVMTMRAN
                     TTRHPEGKTCNVAGMIEGTDPILKDEVIIIGGHLDAVGNGGGLLVAGGLDNASGVIDI
                     MAAAKALAESGIKMKRTVLFLLIGGEEVGLLGSKHYTKNPVFPKEKTVTYINLDMVGN
                     GTGLAASASESCQGLLEYFKNANEKYIHRPMRTSISRGVFYGRPRSDGLVFLADGFRT
                     MNLSTTDGVKPVYYHLPGDDETAVTPEIMEDVAKLIYLSFIEMANAETLNY"
     gene            4745..5836
                     /locus_tag="PLA17_07495"
     CDS             4745..5836
                     /locus_tag="PLA17_07495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK85298.1"
                     /translation="MAYRVLFISDISLSPFTTLTDRMLIRGLRARGVDITVVTQRRVP
                     ETQELEEEGVKVEYIIFDKKISRSVITRLRSIIDKEKAELIHVTFGKAMTNAIMAARG
                     RKVKIIGYYGSLSLHWHDPSAWLTFLSPRIDRLICASDAVEAHAKKQLPPWRRNRTER
                     IYRGYNPEWFKDLIPVTRSEIGVRDDEFLICSVGILRRVKGIRYLTEAAGLLPKEMPF
                     RIILIGDGTDSPDTLRLAAESGVPERFIHKGHLNNPPAWMAACDLYVQPSLSEGLGRA
                     ISEAMCLGKPVIVTDGGGAKELFARGDNGFVVPGGSAAELAKAITACWKNRDSLAATG
                     EKARETIMKDFHHDETIAKTYDLYRELLG"
     gene            complement(6034..6813)
                     /locus_tag="PLA17_07500"
     CDS             complement(6034..6813)
                     /locus_tag="PLA17_07500"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HPK85299.1"
                     /translation="MDPGQVAIITGASRGIGRAITLALALEGYDVAAIARTSDSEGML
                     SLQQEVEALGAQFFPIGLDVANTNLHDEVILSILDRYGRIDLLINNAGIAPIERKDLL
                     DLDEETYDRVLSVNLRGPVFLARRVAREMIWLREKIKDYRPAIMFVTSFLGNTSFTHG
                     VDVCISKAGLSMAALVFADRLSEEGINVYEIRPGLIKTELSTRSKYKFDRLITEGLIP
                     QKRWGTPEDVAKAVASLARGDWAFSTGTIFEVSGGMNIKSI"
     gene            complement(6831..>8374)
                     /locus_tag="PLA17_07505"
     CDS             complement(6831..>8374)
                     /locus_tag="PLA17_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85300.1"
                     /translation="LIPRISRFLEQNLLELNIDGEKIRGYRSPEARSIWIRHYSDMMR
                     ALRYTEADLISVVRHFADNQSRNGRIFDYFTAFPEKRPYERENWIKYVRIPVAADTEF
                     RFIKAAWLAWQATGDDAFIRNLMPTFERALAYVMEECYFDKEKYLFRRPYTIDTWGFA
                     YTAGRHDWLQYQIDDNTFWGYFHGDNSGYYEVFHFMSYWYNLFGDKVRSALWRDRAMR
                     LRKSLNTHCFNGSFYTHFVKQSAVTIEGVDEVSQLSLSNPAAINRGAASPRIATAIIN
                     EYMKRRKATTAFAEWFSIDPPFPSGIFGDQKFSKGAYCNGGIMPLAGGELARAALEYG
                     FEQYGIDILTRYYDLISRNGETYLWYFPDGKPSTVEASTSPDAEPTDGWGSSAMLYAL
                     VEGLAGVQDKDKLFRRVRLCPKWLSAGVEKAEVKIDYKSSGAYVRYDYIHTHDQLTIN
                     LSGSYAYIQLSLPVPENYTVGDLMIGGKKKKYIMASFNQGNYLIADADIKGDCEIILD
                     LRKRN"
BASE COUNT         2207 a         2230 c         2096 g         1841 t
ORIGIN      
        1 acttgaacct gggaccccct gattaacagt caggtgctct aaccaactga gctactgagg
       61 aagattctat ggctccgaaa agcgtggcaa aattaaccgc tttaaacgaa ttaagcaata
      121 tctttgtcaa aattttcctg atgaagaaga ttctgggtat cggtaacgct ctcgtcgatg
      181 taatgacaat tatcccggac gaaacctgtc tcggacggtt cagcctccca aaaggaagca
      241 tgacaatggt cgacgcacac cgttccggcg agatcaaaca ggccataaat ggcatgaaaa
      301 gaaccctcgc ctccggcggc tcagcaggaa atacaatgta cggcctcggt ataatgggcg
      361 tcaactcatc attcatcggc aaagtgggca gagacgaaat gggcaacttc tacgaaaaag
      421 atatggtgga cgccggcctg actcccgtac tgatacgctc atcacagtcg cctacaggaa
      481 ccgcggtggc actggtcact cccgactccg aacggacttt cgcaacacac ctcggcgccg
      541 caaccgaact gtcagctgac gaactcaata tcagctattt caaggggttc aacatacttt
      601 acatcgaagg ctacctcatc tataacctcc ccctggccga aaaagccttt caacttgcaa
      661 aagaaaaccg catgtgcgtg gctcttgacc tggctagttt taatgtggtc accgaactgc
      721 tgccttcatt tgaccggctc gtgagagaat acgttgatat tgtcttcgcc aacgaggagg
      781 aggcaagagc attcaccggc ggcctgaatc ccagggaagc gctcgacgcc atagccggca
      841 aatgcaagat agccgttgtc aaaatgggcc ctgaaggatc atggataaag agaggagacg
      901 aggtgatcaa ggtcgaagcc ctgaaagtca aggcagttga cacaaccggc gcaggcgacc
      961 tctacgctgc aggattcctc tatggatttt caaacggatt cagcctcgac aaatgcggtg
     1021 tattcggatc aatcctggca ggaaaagtta tagaggtggt gggggcacgc atgccggaag
     1081 agaaatgggc ggaagccaga aagctggttg acacagtggc ccgggaataa aacagcaaat
     1141 gctcagcgaa atcttctgac cggactcctg tcgtccggac agttacaact gcccaggcgg
     1201 atgaacctgt aaccaacccc cagggtcacc cctccgttaa gcagggtctg tggatgatct
     1261 tcaggtgaaa aatgcagcag tccgtactgt gccacataag ccctgaccct gatgaacata
     1321 tctgacttga accggtactg gattccggct ccgaccgttg ctcccactcc gctcatatcg
     1381 tcaacccggg aagaacctgt ttgcactgca accagagcgc ccgcgtcggc aaaaaaccat
     1441 ttcaggaagt cggcccgcac catcaccggc aacgtcacca ggccaaaaga ccccggtgtg
     1501 tccgtaccac ctatgcccgg cgccggactg gtataataat actgatgtga atatgacaat
     1561 cccgttccga tgctgaaatg atcaccaagc agcctgcgca ggtcagcccc tgccgtccag
     1621 aaccccttcc cggtcaccga tcccgatcca accagctcct gaaacctgaa gatatcattg
     1681 cctccgccac cggcgaaacc tgatatttca ccccaccagg tcttctgtcc gctgcatatt
     1741 cctgaaacaa aaagcaacgt aaaacagatc aaaaatgttc cacttctaaa catgcgaatg
     1801 cgtctatttt ataattattt tgtcactctt cgccctgcaa aaggcgtacc aagcgataaa
     1861 cgcgaagcat acaagaggta ccgcaaaacc gatagccatt cccgacacat cggcaatccg
     1921 gcccattatc accgggcaga tggcgccgcc gacaatggtc atgaccagca ctgacgaccc
     1981 cttcttggtc atcgccccca gatccttcaa ccccagggca aaaattgtag gaaacatgat
     2041 agacatgcag aagtagttca ggtagagagc aacgagggaa ggccatccga gacccagcat
     2101 cacaagcacc atggctgcgg tgttgatcag cgcatatatc cccagcatct gcctcgggct
     2161 gaaccagctc atgaagagtg agccgacaaa ccggcctgac atgaaaaaga caaagccaag
     2221 tgagaggaga taagctgcct tctcaggaga gtatcccggc attgtctcca ccacatagtt
     2281 gataaagaag ctgtttatgc ctgtctgcgc agccacatag agaaactgcg ccacaacagc
     2341 atacctgaaa tgccttaccc tgatcaaccc gcggtaagta cctctgtcgc cgccactgtc
     2401 atgctcaccc tcatgaatct caggcatccg ggtgatgaca aacataaagg cgacaaccag
     2461 caccacaccc cctatcagca tatatggcaa tgccatggaa gagaagttgt tggcgctatc
     2521 gtcaccgttg cctccaagga tcagcatccc tcccagcgcc ggcccgataa tccatcccag
     2581 tccgttgaac gactgcgaga gggtgagccg ctgctcagag gtttccttcg gtccaagcac
     2641 gctcgagtaa ggattcgcag cagtctcaag gaaggtgagt ccgcaggcaa tgataaagag
     2701 gcatagcaga gtaaattcaa atgtccggat gaatgtggcg ggccagaaga gaaaagcccc
     2761 tgcagcatac agcaacagtc cggtaatgat acccttcctg tatcccaccc tgctcatgaa
     2821 gagcccggcc ggaatggcca ttatgaagta accgccgtag agtgcagcct gcaccagccc
     2881 tgacctcgcc ctggtaatgc cgagtatatc ctgaaaatgt ttgttcagca cgtccagaag
     2941 actgtgtgca acaccccaca ggaaaaagag gctcgtaaca agtatgaacg gcacaatgaa
     3001 ttcacgggtc acaagccgcc tggttggttt cgaaagagtt tcgttagtca ttgacaggaa
     3061 ttttaccttg caatatttat cataatcctt gaaatataca acgttccggg cagatggttt
     3121 tgtatccgca atacctgttt atcagcatgt ctgacgaatc tgaagcttaa aacaatatat
     3181 ttgcacaggt tagctgacca ttgaacttaa acataataaa aacatgcgaa cactcttact
     3241 gacagtcgcc atcttgcttc tggctgcctt cacacgcact gctcctgcac aacagacaga
     3301 tgaacagctt attctcaagc atttccattc aatcagcagc atagagatag ccgagtggat
     3361 ggcagagatc tgctcaccga aatacaacgg acgtctggcc ggcacccccg aatacattgc
     3421 ctcggcagag tggatggccg gtaagttcag agagtggggc attaaacccg ctggtgataa
     3481 tggctcttac tttcagtggt ttgaccaccc ctatacagtg gtcaacgagc ccggggaact
     3541 gaagctgcac atcacgcaaa aggatggcgt gaccatagta aaaaactaca aggcgccgta
     3601 tgacttctac cctggcatga actcaggcaa cggcgaggtg accgcagagg tggtatgggt
     3661 gggatatggc accacagccc ccgaactgaa ctatgacgat tataaaaacg tggatgtccg
     3721 gggaaagata gtgctgatga accgcgatgt tcccttcaag gatacccgtg atcctgagta
     3781 tgcaaaatgg gtgaaatact gctatcacca ctacaaggct gagaatgccg cccgtcatgg
     3841 tgctgctggc atgctttaca tcgacggcaa cagcgccaac ccgaacatct cttacattga
     3901 aaacatggtt gttacgggta ttggcccgga gcccctttcg gacatcttcg ccggcctcgg
     3961 taagaacaac aagacccttc ttgaccaggt tgacaaaaca tttaaaccgg catcctttgc
     4021 caccggcaag gttatgacga tgagggcgaa caccacccgt cacccggaag gcaaaacctg
     4081 caatgtggca ggaatgattg aaggaaccga tcccatcctg aaagacgagg tgataatcat
     4141 cggagggcat cttgatgcgg ttggcaatgg aggcggactg ctggttgccg gagggcttga
     4201 caatgccagc ggggtgattg acattatggc tgccgccaaa gcactggcgg agtcagggat
     4261 aaaaatgaag cgcacagtgc tcttcctcct gattggtggt gaagaggtag gtctgctggg
     4321 cagcaagcac tacactaaaa accccgtttt ccccaaggaa aaaaccgtca catacataaa
     4381 ccttgacatg gtgggcaacg gcaccggact ggctgcaagc gcctcagaat catgccaggg
     4441 cctactggag tactttaaaa atgccaatga aaaatacatt cacaggccta tgagaacatc
     4501 catctcgcgg ggagtgtttt acggcaggcc gcgaagcgac ggactggtat tccttgctga
     4561 cggtttcaga accatgaacc tctcaaccac cgatggggtg aaaccggttt actatcacct
     4621 gccgggcgat gatgaaactg cagtaacccc ggaaatcatg gaggatgtgg cgaaactcat
     4681 ctatctctct ttcattgaga tggctaatgc cgaaacactt aactactgaa gcacacacca
     4741 atgaatggca tacagggtcc tcttcataag tgacatctcg ctctcgccgt tcaccacgtt
     4801 aaccgacagg atgctgatcc gtggcctgcg tgccaggggt gttgacataa ctgtggttac
     4861 ccagaggcga gtacccgaaa cgcaagagct ggaagaggaa ggtgtaaagg ttgagtatat
     4921 catttttgat aaaaaaatca gccgctctgt catcacccgg ctgcgcagca tcattgacaa
     4981 agaaaaagca gagctgatcc atgtgacctt cggcaaggcc atgaccaacg ccattatggc
     5041 tgccagggga agaaaagtaa aaatcattgg atactacggc tcgctaagcc ttcactggca
     5101 tgacccgtcg gcatggctga cattcctgag cccgcgcatc gacaggctca tctgtgccag
     5161 tgatgcggtg gaagcccacg caaaaaaaca gctcccgccg tggcggagga acaggactga
     5221 gcgcatctac cgcggatata acccggaatg gttcaaggac ctcatacctg ttacacgaag
     5281 tgagatcggg gtacgtgatg atgaattcct gatctgcagc gtcgggatat tgcggagagt
     5341 aaaaggtatc aggtatctta ccgaagcggc cggactgctc ccaaaagaga tgccgttcag
     5401 aatcatactg ataggtgacg gaaccgattc acctgacacc ctcaggctcg cggcggagag
     5461 cggagtacct gagcggttca tccacaaggg acacctgaat aatccgcctg catggatggc
     5521 ggcgtgtgac ctttatgtgc agccatcgct cagcgaaggg ctgggaagag caatctcaga
     5581 ggcgatgtgc cttggcaagc cggtgatagt gactgacggc ggaggggcaa aagagctttt
     5641 cgccaggggc gataacggct tcgtggtgcc gggaggctcg gctgccgaac tggccaaagc
     5701 catcaccgcc tgctggaaaa acagggactc cctggccgcc acaggagaaa aggccaggga
     5761 gaccatcatg aaggattttc atcacgacga aaccatcgcg aaaacctatg atctataccg
     5821 tgaactgctt ggatgataac taatgttggc tgtgttcaat atctcgctgc ataagccaca
     5881 agcttctact gtaacgacag aagcacaatg gcaaaatctg ccatggataa aatagcctct
     5941 ttcaggttaa gccactgaga agcaggtttt gctcaacaaa agttgaatga tactgtgaaa
     6001 tggcaaaacc ctgatgcggc aaatcgttga ttattaaata ctcttgatgt tcattccgcc
     6061 gcttacctcg aatatggtac cggtagagaa tgcccagtcg ccccgtgcaa gtgatgccac
     6121 agcttttgca acgtcttccg gcgtacccca tcttttctgg ggtatgagcc cctcggtgat
     6181 aagccggtcg aatttgtatt tgctccgtgt gctaagctcg gtctttatca gtcctggcct
     6241 gatctcatag acattaatcc cctcctcgct gagcctgtcg gcgaagacaa gcgctgccat
     6301 gctcagacct gcttttgaga tgcagacatc aaccccatgg gtgaatgagg tattgccaag
     6361 gaatgatgtc acgaacatta tagctggccg gtaatctttg atcttctccc tgagccatat
     6421 catttccctt gccacccttc ttgccaggaa taccggacca cgcagattga cactgagcac
     6481 gcggtcatag gtctcctcat ctaggtcaag gaggtctttg cgttcaatgg gcgctatgcc
     6541 cgcattgttg atgaggaggt cgattctgcc ataacggtca aggatgctca gtatgacttc
     6601 atcatggagg ttggtattag ccacgtcgag ccctatggga aagaattgtg cgccgagcgc
     6661 ctcaacctct tgctgcaggg agagcatacc ttcgctgtca gaggtacgcg cgatggccgc
     6721 cacgtcataa ccctccaaag ccagggccag agtgatggcc ctgcctatcc ccctgctggc
     6781 tcctgttatt atagctacct gtccggggtc catatttcac acttttaggg tcaattcctt
     6841 ttccggagat cgaggatgat ttcgcaatca ccttttatat cggcatcggc aatcaggtaa
     6901 ttgccctggt tgaatgatgc catgatgtac tttttcttct tccctccgat catcaggtca
     6961 cctacggtat agttttcagg aacgggaaga gagagctgaa tgtaagcata gctccctgaa
     7021 aggtttatgg tcagttgatc atgggtatgg atgtaatcat acctgacata agcccccgaa
     7081 ctcttatagt caatcttcac ctctgccttc tccactcctg ccgataacca cttggggcac
     7141 aggcggaccc tcctgaagag cttgtccttg tcctgtacgc cggccagacc ttccaccagg
     7201 gcatagagca tggctgaaga tccccatcca tcggtcggtt ctgcatcagg tgaggtgctg
     7261 gcctccaccg ttgagggctt cccgtcaggg aagtaccaga gataggtttc gccgttgcgt
     7321 gaaatgagat cataatagcg cgtcagtatg tcaatgccat actgttcaaa tccgtattca
     7381 agcgcagccc tggccagttc tcccccggca agtggcatga tgccaccatt acagtaagct
     7441 ccttttgaga acttttggtc accgaaaatc cctgagggga aaggcgggtc aatggaaaac
     7501 cactcggcaa aggcggttgt ggctttacgc cttttcatgt actcatttat gattgccgtg
     7561 gctatgcgcg gtgaggcagc gcccctgttg atagcggcgg gattgctcag gctgagctgg
     7621 ctgacctcgt caactccctc aattgtcacc gcggactgct ttacgaaatg agtatagaat
     7681 gatccgttga aacaatgggt gttcaggctc ttgcgaagcc gcatagcgcg gtcacgccag
     7741 agtgccgagc gaaccttgtc gccaaacagg ttgtaccagt atgacatgaa gtggaatacc
     7801 tcatagtacc cgctgttgtc accgtggaaa tatccccaga aagtgttatc atctatctgg
     7861 tactgaagcc agtcatggcg ccctgccgtg tatgcaaaac cccatgtatc aatggtatat
     7921 ggcctcctga aaaggtattt ctccttgtcg aaatagcact cctccataac ataggcaagc
     7981 gctctctcaa atgtgggcat caggttgcgg atgaaggcgt catcgcctgt tgcctgccat
     8041 gccagccatg cagccttgat aaacctgaac tccgtgtcag cggctacggg aatccggaca
     8101 tatttgatcc agttctcccg ctcgtaaggc cttttttcgg gaaatgcggt aaagtaatca
     8161 aagattctgc cgttgcgcga ctggttatca gcaaagtgtc ttaccacgct gatcaggtca
     8221 gcctcggtat agcgcagagc gcgcatcatg tcagagtaat gcctgatcca tatggaacgg
     8281 gcttcaggcg acctgtaacc tcttatcttc tcaccatcaa tgttcagttc gaggagattc
     8341 tgttccaaaa acctgcttat tctcggaata agct
//
LOCUS       DAOPBK010000113         8366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7900_length_8366_cov_4.487908, whole genome shotgun sequence.
ACCESSION   DAOPBK010000113 DAOPBK010000000
VERSION     DAOPBK010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7900_length_8366_cov_4.487908"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLA17_07510"
     CDS             complement(<1..285)
                     /locus_tag="PLA17_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85301.1"
                     /translation="MMITRNPEDSVHDELFDGKEKTSESAEENAEMVDAAGQPAVDSV
                     TATGEDIPCDAVNEKEENAATKAEPDKEAEAATEDLSGKEEEAANEVVPEE"
     gene            469..654
                     /locus_tag="PLA17_07515"
     CDS             469..654
                     /locus_tag="PLA17_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HPK85302.1"
                     /translation="MEKKDIIILVVVFATIGFSLYRKYMKKKNESSSGSTPRKKSGRS
                     SLKDQADDYEPYSGGNS"
     gene            complement(673..2901)
                     /locus_tag="PLA17_07520"
     CDS             complement(673..2901)
                     /locus_tag="PLA17_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HPK85303.1"
                     /translation="MDIFTFASSHSFNKMKYPFVRQHDAMDCGPACISMVAMRHGKRL
                     SLETIRRKAWITREGVSFLGLRTAAESIGFRAAGVKLPFGRLREETPLPCIVHWQQNH
                     FIVVNRISEKAVWVSDPAIGRIKMTLDEFRQGWISGEAAGEPAGMALLLEPGPGFEAL
                     HDDPPPKGGFGFLLPYLKPYRKQIILLIAGLIVSSAIQLVFPFLTQAIIDRGIAGRDI
                     KLIWLILIGQLALTAGRLAVEFTRGWLLLHMGTKLNINIVSDFLARLMGLPIAYFDTK
                     LNGDILQRIDDNSRIENYLTSSSLAILFSLFNFVVFGVVLGIYSIRILLVFLAGTALY
                     VLYVTLFMPARERLDNMRFMKMAEAGNRMINIVNGMQEIKLAGNEATNRKEWEAQQHA
                     IYNTRIRGLRILQFQTAGGTLIHESVNIIITVISATLVINGSMTLGMMLAVQFITGQL
                     NGPVSQIVDFMRATQDARISLERLAEVHALTPEEESVSADAAEMPGKADVGVRNLSFR
                     YEGPGSPWVLRNLDLYIPAGKVTAVVGESGSGKTTLLKMLLGFYRPEEGDIYIGGTRL
                     EEIRISSLRKNTGVVMQDGYIFPDTIMGNIAPGEENPDAEKMAEAVRVARLKPMLDML
                     PSGLQTRVGQGGHGLSQGQRQRILIARVVYKRPALVLLDEATSALDASNEKMIVENLA
                     EFYAGRTVVVVAHRLSTVRHADNIIVLGGGRVVEEGTHASLTALKGTYYRLIRDQLEM
                     GR"
     gene            3000..3566
                     /locus_tag="PLA17_07525"
     CDS             3000..3566
                     /locus_tag="PLA17_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK85304.1"
                     /translation="MGSFRKYSDLDIIDGVRRQDNRILSYLYNSYYKMVSDYVRKNGG
                     SDDDAGEVMQESVVSLYRQITSGEFSVKSDLKGYFFGVARNLWIAQLRHRSRLAYFED
                     EPADLSLNEDQYKATLERIVARSFALLAEDCQTVLTLYGDGLSYEEIADRMGLKNAVY
                     ARRKKYLCKEALMKIIKTDPEYHDLGSL"
     gene            complement(3545..6646)
                     /locus_tag="PLA17_07530"
     CDS             complement(3545..6646)
                     /locus_tag="PLA17_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPK85305.1"
                     /translation="MVPDTVLAAGAGKLTIAGFYKWVGSAGGKESGSVPFPAADKQWD
                     TSGGARLREKTDSLAAFLEERDYCADPERLAAEITALLEATETKDLLLLSDAYYFVGV
                     HLYQSNLYIKASDCFSRSVTYREKLGLADRRYANGLINMAVSLLRSGDFSRAYPLGIK
                     ALEAKRALLGSDSSGLATNYLNLASICLELNDNEQAVTLAEAGLTVSGIYPDLVPPRV
                     RADLYHVIGLSLYRTQEYNKSLVYCREALKIFESDPAGNAGSRILLHNTIALVYRRLG
                     QSDKAEEYFRRGLVIKEEKNAEDKEFLYINYSNLLGQSGQISEGERVLEEGLARVKSI
                     YGPESREYFMMLVSYADFINSNHGGSARSLALYEQCFSYVRANPWDVSASKFLTLKYV
                     QSLAGIGKYAEVLRITDETDFLTGAYSPSAGAEAGSGSNLVAPAVFSENDIGILEIRY
                     AALNALANDAHDGQYDKQAVETGKMIVSLYDRQRLELSEDESRTSFSAYSRDIYTGII
                     GNYARLYEADHSREALEGIFEYSERSKVAGFLASIRELNAARFSLPAELAGLEADIRR
                     QTGFYRELIASEQLKAVPDSQRLATWESVTFSLLRSRDSLNRIFKEQYPSYYNLKYRN
                     EVASTADVGRVIGRRSNLLSYVLTSDRLYIFVANRRGNEVITREIDSGFFDALHRFRR
                     ILSTSPMTTGSRARFNEYMDLAYSLYRILIEPAEPYLVGDKIVISPDNILSYLPFETL
                     VTEEFRSPELLYREAPFALKKYRFSYIYSVTLSSETMQRTRRLNNRLVAFAPTYEGLE
                     VSDSVLAVWPTLSGEIRELPAALIEAEDAVSQCGGRAYLAGDASEETFKREAPGYDII
                     HLAMHALVDDSRPAFSKMLFAGMEEGPDDGMLNTYEVYRLPLKAMMVVLSSCNTGSGT
                     LAGGEGILSLARGFLYAGSRSAVMSMWEVDDASASEVIHSFYKNMRSGQTKSSALRNA
                     RLKFLRSADQGRSHPYYWSTLVIYGDDTPLWYNRVTLYISLLLLLLVVTVLVALIYRE
                     PRS"
     gene            6915..7046
                     /locus_tag="PLA17_07535"
     CDS             6915..7046
                     /locus_tag="PLA17_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85306.1"
                     /translation="MKTLIENFSDFILSNEEMINVRGGNGIKVQVGDPTRPPVIIEI"
     gene            7239..7985
                     /locus_tag="PLA17_07540"
     CDS             7239..7985
                     /locus_tag="PLA17_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85307.1"
                     /translation="MKTNDVNEVIENWLGGHADRSAQDWLIDEMKKDAALAREVTLRK
                     KTDEILSDREVINLRHKLTAIEMRRRNAGTVRRTVIRSAKYAAAVALIAILSTALYLM
                     LKPSYSPEGLYSANYSRYESPGVVRSAVSAENTLMENAIASYTAREYDKAIIYLEKLI
                     NTGQEEMEPVFMHGMANMEVKNYPVASGSFNRVIEHNDNLYLEDAAWYLGLCYMMTDN
                     KDKAVRQFSAIASSKSRYCKKAARLVRQLK"
     gene            8019..>8366
                     /locus_tag="PLA17_07545"
     CDS             8019..>8366
                     /locus_tag="PLA17_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPK85308.1"
                     /translation="MFEIHCNARNETGLKAANGHEKLDKMINQQNIIAGVSHEVRTQM
                     NSIVAFSYLINNERFSESERREFTEQIISSCEQLIGLFENYFDTAALESGSATENLRE
                     TDVTSLFESLTSDF"
BASE COUNT         2143 a         2126 c         1983 g         2114 t
ORIGIN      
        1 ctcttcaggt acgacttcat tggcagcttc ctcctctttg ccagataggt cttcagtggc
       61 agcctctgcc tccttgtcag gttcagcttt agtggcagcg ttttcctctt tctcgtttac
      121 ggcatcacag ggaatatctt cgcccgttgc ggttacggaa tcaacggcag gctgcccggc
      181 cgcgtcaacc atttcagcat tctcttccgc agattcgctg gtcttttctt taccatcgaa
      241 tagttcatca tgaacagagt cttccggatt tcttgtaatc atcattaatc ctttttcttc
      301 agtgcttcat caaaggagga tgggatgaac ctctcccctt ttttttcaaa caacgaaaat
      361 aatggattat tggtaaaact caaaccaata ccctatttta aatgaggcgc attgccggca
      421 gagcttcaaa ttggttattt ttgtctgtca gattcactat catctgtcat ggaaaagaaa
      481 gatatcataa tccttgtggt ggtgtttgcg actataggtt tttcactgta cagaaagtac
      541 atgaagaaga agaatgaaag ctcgtccggc agcacaccac gcaaaaaatc tggcagaagc
      601 agcctgaagg atcaggcgga tgattacgaa ccctattccg gcgggaatag ctgagagcag
      661 tgaaataccg gtctaccggc ccatctcaag ctggtctctg atgagcctgt aataggtacc
      721 tttcagggcg gtaaggctgg catgtgttcc ctcttcgaca accctgcctc ccccaagtac
      781 gattatgttg tcggcatgcc gcactgtgct gagcctgtga gctaccacca ccactgttcg
      841 cccggcatag aattctgcca ggttttcgac tatcattttt tcgtttgatg catcaagagc
      901 acttgtggcc tcgtcaagga gaacaagtgc tggtctcttg tatacgaccc tggcaatcag
      961 tatccgttgc cgctggccct ggctcaggcc gtgtcctccc tgcccgacac gggtctggag
     1021 ccctgatggc agcatgtcaa gcatgggctt cagcctggcc acccttactg cttcagccat
     1081 tttttcagcg tcaggattct cctctccggg agcaatgttg cccattatgg tgtcaggaaa
     1141 aatatacccg tcctgcatca ctacaccggt attctttctg agtgatgaga tccttatctc
     1201 ttccagcctc gtacctccga tgtatatatc accctcctca ggcctgtaga aaccaagcag
     1261 catcttcagc agggtggtct tccccgaccc gctttctcct accacggcag tcaccttgcc
     1321 tgccgggatg taaaggtcga ggttacgaag tacccagggt gaacctggtc cctcgtatct
     1381 gaatgaaagg tttctaaccc caacatctgc tttcccgggc atctctgcag catctgctga
     1441 aacggattct tcctcgggtg tcagggcatg cacctcagcc agtcgttcaa ggctgatcct
     1501 tgcatcctgg gtagctctca tgaaatctac tatctgtgag accgggccgt taagttgccc
     1561 ggtgatgaac tgcaccgcca gcatcattcc cagggtcatt gagccgttga tgacaagtgt
     1621 ggcggagatt acggtaatga taatattgac agactcgtga ataagcgttc ctccggcggt
     1681 ctgaaactga aggatcctca atcccctgat acgggtattg tatattgcgt gctgctgcgc
     1741 ttcccattcc ttcctgttgg tggcctcatt gccggcgagc tttatctcct gcatgccgtt
     1801 gacaatgttg atcatcctgt tcccggcctc ggccatcttc atgaaacgca tgttgtcaag
     1861 gcgctcgcgg gccggcatga aaagagtgac atagaggacg taaagggcag tgcctgcaag
     1921 gaacaccagc agtatcctga tactgtagat tcccagaacc accccgaaaa ccacaaagtt
     1981 gaaaagggaa aagagaatgg caaggcttga agaggtcagg tagttctcta tacggctgtt
     2041 gtcatcaatt ctctgaagta tatcaccgtt cagcttcgtg tcaaagtagg caataggcaa
     2101 tcccatcagg cgggcaagga agtctgaaac aatattgata ttcagctttg tgcccatatg
     2161 cagcagaagc catccccgcg tgaactccac agcaagcctg ccggcggtaa gggcaagctg
     2221 gccgatcagt ataagccaga taagcttaat atcccgtccg gctatccccc tgtcaatgat
     2281 tgcctgtgtc aggaagggaa agacaagctg gattgcactg gatacgatca aaccggcaat
     2341 caggaggatt atctgctttc ggtacggttt gagatagggc agcaggaacc cgaagccgcc
     2401 ctttggtggc ggatcatcat gcagcgcttc gaatccgggt ccgggctcca gcaacagtgc
     2461 catccctgcc ggttcaccag ccgcctctcc tgatatccat ccctgcctga actcatccag
     2521 ggtcatcttt atcctgccaa tggcagggtc actcacccag acagcttttt cacttatcct
     2581 gttgacgact atgaaatgat tctgctgcca gtgcacaatg catggcagag gtgtttcctc
     2641 cctgagcctg ccgaagggca gctttacccc tgcagccctg aacccgatac tctcagctgc
     2701 agttctcaga cccagaaatg ataccccttc acgggtgatc catgcctttc tccttattgt
     2761 ctccagtgag agacgtttgc catgccgcat tgccaccatg ctgatgcatg cgggcccgca
     2821 atccatggca tcatgctgcc tgacaaacgg atatttcatc ttgttgaagg agtgactgga
     2881 tgcaaatgta aatatatcca attaaagctg actcatacag gtgattcttt aagtatgtgc
     2941 cccggcatat ttactgtatc gattgcggaa attgtatatt tgccaattaa gtcacttgtt
     3001 tgggatcatt caggaagtac tcggaccttg acatcattga tggagtcagg cggcaggata
     3061 accgtatcct gtcatacctg tataactcct attacaagat ggtaagtgac tatgtcagga
     3121 agaacggtgg ttctgacgat gatgcaggcg aggtaatgca ggaatcggtt gtaagcctgt
     3181 acagacagat cacatccgga gaattctcgg taaagtcaga cctcaagggc tatttttttg
     3241 gtgtggcgag gaatttatgg atcgcccagc tgaggcaccg ctccaggctg gcttattttg
     3301 aggacgaacc tgccgatctt tcattaaacg aggatcagta caaggctacg ctcgagagaa
     3361 tagttgcccg ttcattcgcc cttctggcag aggattgcca gacagtgctt acactctatg
     3421 gcgacggact ctcttacgaa gagattgctg acaggatggg gctgaaaaat gccgtctatg
     3481 ccagacgaaa aaagtatctc tgcaaggaag ccctgatgaa aatcatcaag acagatccgg
     3541 aatatcatga tctgggttcc ctgtaaatca aggccaccag caccgtaacc accagcaaca
     3601 gaagaagaag ggatatatat agggtgaccc tgttatacca caggggggta tcatctccgt
     3661 agatgacaag cgttgaccag taatatggat gagaccgtcc ctggtcagca ctcctcagga
     3721 atttcagtcg tgcattgcgc agtgcggaac tttttgtctg tccgcttctc atattcttgt
     3781 aaaaggagtg tattacctcc gaagctgagg catcatccac ctcccacatt gacatcactg
     3841 ccgagcggct cccggcatag aggaatcccc tggcaagact gagtattccc tcaccgcctg
     3901 ccagggtgcc actgcctgta ttgcatgagc tgaggacaac catcattgcc ttcagaggga
     3961 gcctgtaaac ctcgtatgtg ttgagcattc cgtcatcagg gccctcctcc atgcctgcaa
     4021 agagcatttt tgaaaaggca ggccggctgt catccaccag ggcatgcatt gcaaggtgaa
     4081 tgatatcgta acctggcgct tcacgcttaa aggtctcctc gctggcatcc ccggccaggt
     4141 atgctctgcc gccacactgg ctgacagcat cttcagcctc aataagggca gcgggaagct
     4201 cccgtatttc tccactgaga gtgggccaca ctgcaagaac agaatcagag acctccaatc
     4261 cttcatacgt cggagcaaag gcaacaagcc tgttgttcaa tctccgggtt ctttgcatcg
     4321 tctctgacga aagggtgaca ctgtaaatat aagagaaccg atacttcttc aaggcaaacg
     4381 gagcctcccg gtacaacaac tcaggactgc gaaactcttc ggtcacgagc gtctcgaaag
     4441 gaagatagga aaggatgttg tcaggtgata taactatctt gtcaccaacg aggtatggtt
     4501 cggcaggctc aatcaggatg cggtaaaggc tataggcaag atccatgtac tcattgaacc
     4561 gggcacgtga tccggttgtc atggggctgg ttgacaagat cctgcgaaag cggtgcaggg
     4621 cgtcgaagaa acctgaatca atctcccggg tgataacctc attccccctt ctgttggcaa
     4681 caaagatata aagcctgtct gatgtaagga cataactgag cagattcgac cttctgccga
     4741 ttacccttcc cacatctgcg gtcgaagcca cctcattcct gtatttcagg ttatagtacg
     4801 aaggatactg ttctttaaag attcggttca gtgagtcacg tgacctgagc agactgaagg
     4861 tgacgctttc ccatgtagcc agcctctgcg aatcaggaac tgccttaagc tgctcactgg
     4921 caatcagctc cctgtagaat cctgtctgtc tcctgatatc cgcttcgaga ccggccagct
     4981 cggcgggaag ggaaaaacgt gcagcattaa gttcacggat cgaggcaagg aatcctgcca
     5041 ccttactcct ttcggaatac tcgaatattc cttcaagggc ttcgcggctg tgatcagcct
     5101 cataaagcct tgcatagttg ccgataatgc ctgtataaat atcccttgaa tatgcgctga
     5161 aactggtccg gctttcatcc tcactcaact caagacgctg acggtcatac agggaaacta
     5221 tcatcttgcc tgtttccact gcctgcttgt catattggcc gtcgtgcgca tcattggcga
     5281 gggcattcag ggcggcatac ctgatttcaa ggataccgat atcattctcg gaaaagaccg
     5341 ccggtgccac aagattgctc ccggagcctg cctctgctcc ggcagacgga gaatatgctc
     5401 ccgtcaggaa gtcggtttca tcagttatcc tcagcacctc ggcatatttc cctataccgg
     5461 ccagtgactg cacatacttg agtgtaagga actttgacgc tgagacatcc catggattag
     5521 cacgcacata gctgaaacac tgttcataga gagccagtga gcgcgcactg ccgccatgat
     5581 tgctgttaat gaagtcggca taggaaacaa gcatcatgaa gtattccctg ctttcaggtc
     5641 cgtagatgct ttttacccgt gccagcccct cctccagcac cctttcacct tcacttatct
     5701 gccccgactg acccagtaga tttgaataat ttatatagag gaattcttta tcctcggcgt
     5761 tcttttcctc tttaatcacc agccctctcc tgaagtattc ctcagccttg tcagactgtc
     5821 ccagccgcct gtacaccagg gcaatagtat tgtggagcag aatgcgggag cctgcattac
     5881 ctgcagggtc actttcaaag atcttcagcg cctcccggca atagaccagt gacttgttgt
     5941 attcctgtgt gcggtaaagg ctcagtccga tcacatgata caggtcagcc ctgacccggg
     6001 gcggcacaag gtcaggatag attcccgata ccgtcaaacc tgcttcagcc agggtaacag
     6061 cctgctcatt gtcattcaac tccaggcata tgcttgcaag gttgagataa ttggttgcca
     6121 gcccggaaga gtcactgccc aggagagctc tcttggcctc cagagctttg atccccagcg
     6181 gataagccct ggaaaaatct ccggacctga gcagcgaaac agccatattg atcaacccgt
     6241 tggcatacct tctgtcagca agaccaagct tctccctgta ggtgaccgaa cgcgaaaaac
     6301 aatcagaagc ctttatgtaa agatttgact gatagagatg tacgccaaca aaataatagg
     6361 cgtcagatag cagaagcaaa tcctttgttt cagttgcttc aaggagtgcg gtgatttccg
     6421 cagcaagcct ctccggatca gcacaataat cccgttcctc aaggaaagcg gcaagtgaat
     6481 ctgtcttctc cctcagcctg gcaccaccgg atgtgtccca ttgcttatca gcagcaggga
     6541 atggcactga gccggattct ttccctccgg ctgaacccac ccatttatag aagcctgcaa
     6601 tagtcagttt ccctgcccct gctgccagaa ctgtatcagg caccatgcag aaaactgtca
     6661 aaaacaggac tgttaaagtg attattcctg acttcggacg catcagcgtg gctcttcttt
     6721 cttcctccgg taaaagtata aaacaatctg ttaccggatc tgatttttac tcatattata
     6781 catgcttgtt attcaatatg ttgcattttt tttgaaaaat agttgatttt ttcgtgtcac
     6841 aaaatggcac tttctgacac gtacgataaa agagacaatt aaaccctgat attaattatt
     6901 taaaaattaa agttatgaaa accctgattg aaaacttttc agattttatt ctttcgaacg
     6961 aggaaatgat caacgtacgc ggtggcaacg gaataaaggt gcaagttggt gatccgacca
     7021 ggcctccggt gatcattgag atttgagttg actttaattg ccggttgatc cggcatctgt
     7081 ctttataata caagaggggt taatcagcct ttgctgatga ccctttacgt caggcatacg
     7141 cccggatatt ggtggtgctg tccgcaacag tatggagcgg agtatgagta actatctgat
     7201 tgatagcttt ataggagtgt gaaataatat tatacaacat gaagacaaat gatgtcaacg
     7261 aagtcatcga gaattggctt ggcgggcacg ctgacaggtc tgcacaggat tggctgatcg
     7321 atgaaatgaa aaaggatgct gcactggcaa gagaagtaac gctgagaaaa aagaccgacg
     7381 agattctctc tgaccgcgag gtaattaacc tcaggcataa gctgacagct attgagatga
     7441 gaaggcgaaa tgccggaacc gtaaggagaa ctgttatcag gagtgccaaa tatgcggcag
     7501 cagtagccct gattgccata ttatctactg ccctctacct gatgctgaag ccatcttact
     7561 cacccgaagg gctttactct gccaactact cacgctatga gtccccgggg gttgtgaggt
     7621 ctgcagtcag tgcggagaat accctcatgg aaaacgctat tgcctcatac actgcaagag
     7681 agtatgataa agccatcatc tatcttgaaa agctgataaa tacagggcag gaagagatgg
     7741 aaccagtgtt catgcacggg atggcaaaca tggaggtaaa gaactacccg gtagccagcg
     7801 gatcattcaa ccgtgtcata gagcacaatg acaacctcta tcttgaagat gcagcctggt
     7861 acctggggct ctgttatatg atgactgaca ataaagacaa ggctgtcagg cagttcagcg
     7921 caatagcctc ttcaaaaagc agatactgca aaaaggctgc aaggctggtc cgccagctta
     7981 aatgataaac cagactttaa ctattgttaa ctgttcatat gttcgagatt cattgcaatg
     8041 ccaggaatga gacggggctg aaagcagcga atgggcatga aaaactggac aagatgatca
     8101 accaacaaaa tattattgca ggggtttcac atgaggtgag aacccagatg aactccatcg
     8161 tagctttttc ttaccttatc aataacgaaa ggttcagcga aagtgaaaga cgtgagttca
     8221 ctgaacagat catcagttca tgcgaacaac tcattggatt gtttgaaaac tattttgata
     8281 ctgctgccct tgaatcaggg tcagccacgg aaaacctccg cgagacagat gtcaccagtc
     8341 tgtttgagtc tctaacctct gatttc
//
LOCUS       DAOPBK010000114         8358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7910_length_8358_cov_6.418283, whole genome shotgun sequence.
ACCESSION   DAOPBK010000114 DAOPBK010000000
VERSION     DAOPBK010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7910_length_8358_cov_6.418283"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(519..593)
                     /locus_tag="PLA17_07550"
     tRNA            complement(519..593)
                     /locus_tag="PLA17_07550"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(556..558),aa:Ile,seq:cat)
     gene            complement(725..1333)
                     /locus_tag="PLA17_07555"
     CDS             complement(725..1333)
                     /locus_tag="PLA17_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPK85309.1"
                     /translation="MEGFVKFNCYWRQSGPVITDEQYEIINHWREILFNLDLVGAFEN
                     GVGFGNLSIRIGKSSQFIITGSSTGDIPELEPGHYVKVSSFNIDDNAIMCSGPLKASS
                     ESLTHAAIYMADPGTNAIIHVHSTQLWEELMNKVPTTDPSVEYGTPAMAREMIRIFGE
                     PEVFEKRIIVMGGDRGGLITFGYDLDEAGGVLFDYINRGTSS"
     gene            complement(1339..2505)
                     /gene="mtnA"
                     /locus_tag="PLA17_07560"
     CDS             complement(1339..2505)
                     /gene="mtnA"
                     /locus_tag="PLA17_07560"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPK85310.1"
                     /translation="MKVGERHYRSIWHDKTDRGSVHVIDQRRLPFAFEVMTLRSVNDV
                     YDAIRDMAVRGAPLIGAAAAWGVYLSFLEHSHGNKNHTGIGSYDGEKNSTVTEPPGGR
                     KKEDDNEQAGSGDVYNAVISDAERLKSARPTAVNLAWAVDYMTRVMKFFPDADALRRA
                     AKELCDGEAERSRLIGVNGLPLIEEASSRKGGEVVNILTHCNAGWLACVDYGTALAPV
                     YMAHDMGIRVHVWVDETRPRNQGSRLTAWELLNHGVPCTLITDNAGGHLMQNGMVDMV
                     IVGCDRATASGDVANKSGTYLKALAAFDNGIPFYSALPVSTFDLSIESPAQIDIEERD
                     ASEVSTIDGIRIAPDGVNISNYGFDVTPARLITGLITDRGICRAEKESILKTVS"
     gene            2671..3258
                     /locus_tag="PLA17_07565"
     CDS             2671..3258
                     /locus_tag="PLA17_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPK85311.1"
                     /translation="MVNKEEYRARIIRIASRIFSHYGFRKTTMDEISRALKKGKSSIY
                     YYFRSKEEIFEAVVLYEANQLRRRLTAAIKEVDSPKDKLRNYIYVRMREFAKLSNYYN
                     AVFDKNLDHFDFIEKIRARYDREELAIIRLLVYVGNNRGTFAVEDSEYTSMAIQTMLK
                     GMEVPLFWRRRETDIRDRLEAILTLIFNGILKKQQ"
     gene            complement(3464..4306)
                     /gene="lgt"
                     /locus_tag="PLA17_07570"
     CDS             complement(3464..4306)
                     /gene="lgt"
                     /locus_tag="PLA17_07570"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPK85312.1"
                     /translation="MELQIIPWNVNPEIFRIGNFAVRWYGLLFASGFVFGYYIMRRIF
                     RHEGLTDATLDRLTVYMAIGTIVGARLGHCFFYEPGYYLARPIEILKVWHGGLASHGA
                     AIGILLALWLFVRKEKKPFLWIIDRIVIVVALAGSLIRLGNLMNSEIYGVETTLPWGF
                     VFLRNGETAPKHPTQIYESLAYLITFGILMRIYWKKAGKPLPGLLFGLFLILVFGFRF
                     LVEYIKEDQVAFEAGMKLNMGQWLSIPLMLAGIVLLIWVFTRKPAPKPVTEPWSPQKG
                     VPKR"
     gene            4434..6233
                     /locus_tag="PLA17_07575"
     CDS             4434..6233
                     /locus_tag="PLA17_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HPK85313.1"
                     /translation="MDYGRYISGSLGLTVIATLFVLSCAKISAPTGGPKDMDPPVILK
                     SVPENGSVMFTGKSFTVTFDEYVVLDRISEKFMVSPPMATKPEVKLRGKSLVVTWEEE
                     LADSTTYTFYFQDAIRDNNENNPINNYQYVFSTGPVLDSLSLTGNVFNAFDLEIVEDI
                     TVIMFSNLSDTAPRTMLPAYISRPDPSGGFMISNIRPGDYRLFALKDLNGNTRYDLTD
                     EIFAFGDSVISIAPDNYFGLVPDTVTYRPANATEATKPDKFVYGLHRLYAFREPPKQQ
                     YLTFTERKSSWGISFGLALPSDSGQVSATLPEADSSAWFMEHNAARDTFMIWVTNPDV
                     YGQDLVDVILTFPFTDTTGTIIAKTDTISFRYMKPAAPRGGSGRTPGLNITHNISGKV
                     RPGIVPSFRSATPFAEPDTSLIRLTRTVDSVQTLLPYSFVIDTVSSRLLYMKAQFEPG
                     ATYSLMCRKGAFSDIFGTANDSATYNISVATEEDYGSLMVRLSGYEGKVIIQLTGDRD
                     KVVREVSVTSPGEVSFPLLDKGKYRLKAIYDLDSDGKWTTGDFNAGRQPEPVTYYPDE
                     LEVKINWALEQDWDIGEMYVKNVSLRSKPTTRR"
     gene            6238..7668
                     /locus_tag="PLA17_07580"
     CDS             6238..7668
                     /locus_tag="PLA17_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HPK85314.1"
                     /translation="MSSRRKFIRNSMMAGVALSAGIPSFVTAGTRRWPSARPPVRKRK
                     FRSKAVEETIREIESFLGSDNELAWLFSNCFPNTLDTTVTFSEKEGRPDTFVITGDIH
                     AMWLRDSSAQVWPYVPLAPRDPQLKALIAGVINRQVKCIHIDPYANAFNDGPGQSEWM
                     NDLTDMKPELHERKWEIDSLCYPVRLAHGYWKATGDTSVFDASWSSAMRLIIKTFREQ
                     QRKDNPGPYKFQRVTGWQTDTVAGAGYGNPINPVGLIVSIFRPSDDATIWPFLIPSNY
                     FAVSALRKMGEIAADVTRDEALLADATALAGEVNDALAKYARAVHPVHGEIIPYETDG
                     YFNYNFMDDANVPSLLSMPYLGTIDRSDPLYLRTRKFVLSKSNPYYFSGISGHGVGSP
                     HTGRDRIWPIAIIMKALTASNDHDIITSLRMLKNTHAATGFMHESFMKDDPSKFTRKW
                     FAWANTLFGELVLQTYKTQPELLKEV"
     gene            7680..>8358
                     /locus_tag="PLA17_07585"
     CDS             7680..>8358
                     /locus_tag="PLA17_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK85315.1"
                     /translation="MKEVVAGVDIGGTNTVIGFVDAEGVIHAGGNLSTPDYALADDFV
                     IALDAKLKEMMAENSGLKLIGIGIGAPNANFNKGTIELAPNLRWKGIIPLVRMTEEAT
                     GLRTRITNDANAAALGEMIFGAAKGVKDFIILTLGTGLGSGFVVDGEVVYGHTGFAGE
                     LGHMIVVEGGRVCGCGRRGCLETYASATGLVRTALSLLSDRRDDSTLRELRGSEITSR
                     RIAEAAAA"
BASE COUNT         2077 a         2159 c         2074 g         2048 t
ORIGIN      
        1 ttggcaacag gggaagattg ttccatggga catttctgtc gggattgatt tgatatggca
       61 tttcagacat taattgcggg gaaaattaat ggttactcaa ttcctgacct caaatatagt
      121 gcaaatatat ctgccaaaca ttaaatatct aattatttaa ggccggagtg cgttgttgac
      181 cttaaaaaag ataacaaaaa taaagggtac cctggcaatt tcatgcagga aaaacaatga
      241 ttttcctgag aatccagatt ggttaaatgt taagattgcc cggatggagg ctgctaatta
      301 ttattttggc actgaaatat atctgtatgg atggaatggt gacttctact attggatatc
      361 catcccaata tcatcttgta tgatgtgtga attttatact tataagggtg acaaacctat
      421 ttggacagac gataatttta aagatttccg gaaaaatgct gtaaagagga aagtggtctg
      481 gcgaagagac aactttgttg taacttcttt gttttgagtg ggcccagctg gaatcgaacc
      541 agcgaccccc tgattatgag tcaggtgctg ctaaccatct gagctatggg cccgaaatat
      601 cagggtgcaa atataacatc aaattgaaat atgtgcaaac cttttgcaat gcttaatatg
      661 cagttaaaca accggcgtca cccctgcaat ccggctgctt cgccataatg acccgggggt
      721 cattttacga gcttgttcct ctgttgatgt aatcaaacaa caccccgcct gcctcatcaa
      781 ggtcataacc gaaggtgata aggccaccac ggtcaccgcc catgacaatg atcctcttct
      841 cgaatacctc cggctcaccg aaaatacgga tcatctcccg cgccatcgcc ggggtgccat
      901 actccaccga cggatcagtg gtaggcacct tgttcataag ctcttcccac agttgtgttg
      961 agtgaacatg gatgatcgca ttggtacccg gatcagccat atagatagct gcatgggtaa
     1021 gtgattccga cgatgccttc agcggcccgc tgcacataat cgcgttgtca tctatattaa
     1081 atgacgatac ctttacatag tgccccggct caagctcggg tatatcaccg gtagatgatc
     1141 cggttattat aaactgagac gacttcccta tgcgtatgct gagatttccg aaccctacgc
     1201 cgttctcgaa cgcaccaaca agatcaagat tgaagagaat ctctctccag tgattgataa
     1261 tttcatactg ctcgtcagtg attaccgggc ccgattgtct ccagtagcag ttaaacttga
     1321 caaacccttc catataacct atgatactgt ttttaatatc gattcctttt ctgccctgca
     1381 aattccgcgg tctgtgataa gaccggtgat aagacgcgca ggtgtaacgt caaacccgta
     1441 attggatata ttaacaccat ccggtgcaat tcttatgcca tcaattgtac ttacctccga
     1501 ggcatcgcgc tcctcaatat ctatctgtgc cgggctctca attgagagat cgaatgtaga
     1561 taccggcagt gccgaataaa aaggtatccc gttatcaaat gccgcaagag ccttaagata
     1621 tgtaccactc ttgttggcca catcacccga agccgttgcc ctgtcgcatc cgacgatcac
     1681 catatctacc ataccgttct gcatcaggtg cccgccggca ttgtcggtga tgagggtgca
     1741 gggcactccg tgattgagca gttcccatgc ggtgagacgg ctcccctggt tgcgcggacg
     1801 tgtttcatca acccatacat gaaccctgat tcccatgtca tgagccatgt acaccggcgc
     1861 cagcgccgta ccgtaatcaa cacatgccag ccacccggca ttgcagtgtg tcaggatatt
     1921 gacaacctct cctcccttcc tgctgcttgc ctcttcaatt aacgggagtc cgttcactcc
     1981 tatcagcctg cttctctcag cctccccgtc acataattct tttgctgctc tgcgcaatgc
     2041 atcagcatca gggaagaatt tcatcaccct ggtcatataa tcaactgccc atgcgagatt
     2101 gactgcggtg ggcctggccg acttaagccg ctccgcgtca ctgatcactg cattgtaaac
     2161 atcgccgctg ccggcctgtt cattgtcgtc ctcctttttc cttccgccgg gcggttcagt
     2221 tacggtagag ttcttttctc cgtcgtaaga tccgatgccg gtatgatttt tattcccatg
     2281 gctgtgttca aggaatgaga gatagactcc ccatgctgct gcagctccta tgagaggtgc
     2341 acccctcacc gccatatcac gtatggcatc ataaacatcg ttcacactcc tgagggtcat
     2401 cacctcaaag gcaaaaggaa gcctcctctg gtcaatcaca tgtaccgacc cgcggtcagt
     2461 tttatcatgc cagatactcc tgtaatgcct ttccccaact ttcataaatt tctcttccca
     2521 cacgaattaa tcatcaaaat tactaataat tacactaatt ctgagtattt gaacagttac
     2581 ttaattcaat aacaaccatt ttcgaatatc cgacccgttt gttctacagc attagaatta
     2641 attgattatc tttgcataag aataaaacct atggttaata aagaggaata cagggcacgg
     2701 atcatccgga tagcaagccg gattttcagt cattacggct tcagaaagac tacgatggat
     2761 gagatttccc gggcactgaa aaaggggaag agttcaatct attattattt ccggagcaag
     2821 gaagagatct tcgaggcggt ggtgctttat gaggcgaacc agctgcgccg aaggctgaca
     2881 gctgcaatca aggaggtgga ctcgccgaag gacaagttgc gtaactatat atatgtgagg
     2941 atgagggagt ttgcaaagtt gtcaaactac tacaatgctg tctttgacaa gaaccttgat
     3001 cactttgatt tcatcgaaaa gatcagagcg cgctatgacc gtgaggagct cgccatcatc
     3061 cgcctgcttg tctatgtcgg gaacaacagg ggcacctttg ccgtggaaga cagtgagtac
     3121 acttccatgg ctatacagac catgctcaag ggtatggagg ttccgctctt ctggcgcaga
     3181 cgcgaaactg acatcaggga tcgcctcgaa gcgatactga ccctcatctt caacggcata
     3241 ttgaagaagc agcaataacg gctgttttcc cgtggtgcgg gatcagtgtc taatccgtta
     3301 gtgtctgccg gaggtttttc gcgccgggag ttttccccgc cagagttttt tctataagag
     3361 gttctcccgc tggagttttt ccattgcagg ctttcccgct gcaggtttcc ctgtgagagg
     3421 tttccccttt agaatttttt cccgccgcag gcggttattt cagctacctt ttaggcaccc
     3481 ctttctgtgg tgaccatggt tcggtcaccg gctttggtgc cggctttcgg gtgaataccc
     3541 agatgagcag cacgatgcct gccagcatca gcggtatgct cagccactgg cccatattca
     3601 gtttcatccc ggcctcgaaa gccacctggt cttcctttat atattctacc aggaacctga
     3661 atccgaagac aagaatcagg aacagaccaa acagcagtcc gggcaatggt ttgccggctt
     3721 tcttccagta gatgcgcatc agtattccga aggtgataag gtatgccagt gactcatata
     3781 tctgtgtggg gtgcttcggc gccgtttctc cgttccggag gaatacaaat ccccatggca
     3841 gggttgtctc gactccgtaa atctccgaat tcatcaggtt tcccagacgt atcagtgatc
     3901 cggcgagcgc caccacgata actatcctgt caatgatcca caggaatggc tttttctcct
     3961 tcctgacgaa gagccacagt gccagcagta tgccgatggc cgctccgtga cttgccagac
     4021 cgccgtgcca taccttcagg atctcaatgg gtctggccag ataatacccg ggttcataga
     4081 aaaagcagtg tcccagcctg gcgccgacaa tcgtgccaat ggccatgtaa acagtcagcc
     4141 tgtcgagggt tgcgtctgtc agcccttcat gcctgaagat cctcctcatg atatagtacc
     4201 cgaagacgaa tcctgatgcg aaaagcagtc cataccatct tactgcaaag ttgcctatcc
     4261 ggaagatctc cggattaaca ttccacggaa ttatctgaag ttccatactg ctgatgtttg
     4321 gaatgcaaat ttatgcaaaa aagaaacagt tgtcccattt cactgtaaaa ggcatatccg
     4381 gccccctttg ccccacatat catgtaacaa aatttgatgt aagtttgcag ttggtggatt
     4441 acgggagata catatcaggc agccttggac tgacagttat agccacgctc tttgtgcttt
     4501 cgtgtgcaaa gatcagcgct cctacgggtg gtccgaagga tatggatcct cccgtgatac
     4561 tgaaaagcgt tccggagaac ggcagcgtca tgttcaccgg caagtcattc actgtcacct
     4621 tcgatgaata cgttgtactt gaccgtatat cggaaaaatt catggtgtcg cctccgatgg
     4681 ctacaaagcc cgaggtgaaa ctcaggggca agagccttgt tgtcacctgg gaggaagagc
     4741 tggctgacag caccacctac actttctatt ttcaggatgc catccgtgac aacaatgaaa
     4801 acaacccgat caacaactat cagtatgttt tctctaccgg gccggttctt gactcactct
     4861 cgctgaccgg caacgtgttc aatgcttttg atctcgagat agttgaggat ataacggtga
     4921 tcatgttctc caacctgtct gacactgccc cccgcaccat gctgccggca tacatatcca
     4981 ggccggaccc gtcgggcggg ttcatgataa gcaacatcag gcccggagat tacaggctct
     5041 tcgccctgaa ggacctcaac ggcaatacca ggtacgattt aacggatgag atatttgctt
     5101 tcggtgactc agtcatctcc atcgcacctg ataactattt cggccttgtg cctgacactg
     5161 tcacatacag gcccgccaat gccaccgagg ccacaaagcc tgataagttc gtttacgggc
     5221 tgcacaggct atatgctttc cgggagccgc cgaagcagca atacctgaca ttcaccgaga
     5281 ggaaaagctc atggggcatc agctttggtc tggcattgcc gtcagacagc ggacaggtgt
     5341 cggcaaccct gcccgaagcc gacagttcag catggtttat ggagcacaac gcggcccgtg
     5401 ataccttcat gatatgggta accaaccccg atgtgtacgg ccaagacctg gtggatgtca
     5461 ttctcacctt tccgtttact gacaccaccg gaacgattat cgcgaaaact gacacaatca
     5521 gtttcaggta tatgaagcct gccgccccga ggggaggcag cggaaggacg ccgggactga
     5581 acatcactca caatatttcc ggaaaggtca ggccgggcat cgtgccatcg ttcagatcgg
     5641 ccacaccgtt tgcagagcct gatacatcac tcatcaggct aacccggacg gttgactctg
     5701 ttcagactct gcttccttac agttttgtaa tagatacggt ctcttcccgc ctcctgtaca
     5761 tgaaggcgca gttcgaaccg ggagccacct actcgctgat gtgcagaaag ggagcattca
     5821 gcgatatctt cggaacggca aatgactctg cgacgtataa catctctgta gctacggagg
     5881 aggattacgg aagtctcatg gtcaggcttt ccggctatga gggaaaggtg ataatccagc
     5941 tgaccgggga cagggacaag gttgtacggg aagtatcagt gaccagcccc ggagaggtct
     6001 ctttcccgct tcttgacaag gggaagtaca ggctgaaggc gatctatgac ctggattcag
     6061 acggcaaatg gaccaccggc gactttaacg ctggcaggca gcctgaaccg gtcacatatt
     6121 acccggatga actggaggtc aagattaact gggcgctgga gcaggactgg gatattggag
     6181 aaatgtatgt caagaatgtt tcgctgagga gtaagcccac aacaaggagg taaagagatg
     6241 tcttcacgca ggaaattcat caggaacagc atgatggcag gagtggcgct ctctgccggg
     6301 ataccttcat ttgtcacagc cggaacccgg cggtggcctt ctgcaagacc cccggtcagg
     6361 aagcgcaaat tccgcagtaa ggccgtagag gagacaatca gggagattga gtcattcctg
     6421 ggatctgaca atgagctggc ttggctcttc tcgaactgtt ttcccaacac ccttgacacc
     6481 actgtcacct tctccgaaaa ggagggcaga ccagatacct ttgtgataac gggagatatc
     6541 catgcgatgt ggctgcgcga ctcctcggcc caggtgtggc catatgtgcc gcttgccccg
     6601 cgtgacccgc aactgaaggc tctcatcgcc ggcgtgatca acaggcaggt taagtgtatt
     6661 catatcgatc cttatgccaa tgccttcaat gatggtccgg gccagagcga atggatgaat
     6721 gacctgactg acatgaagcc ggagctgcat gagcgcaagt gggagataga ctcgctctgt
     6781 taccctgtca ggctggctca cggatactgg aaagccaccg gcgacacctc ggttttcgat
     6841 gcctcctggt catcggcgat gaggcttatc atcaaaacat tccgcgagca gcaacgcaag
     6901 gataacccgg gtccgtacaa gttccagagg gtcacaggct ggcagaccga tactgttgcg
     6961 ggagccggat atggtaatcc catcaacccg gtgggcctga tagtatcaat attcaggccg
     7021 tcagatgatg caaccatatg gcctttcctg attccctcga actatttcgc tgttagcgca
     7081 ctacgcaaaa tgggcgagat tgctgccgat gtcacacgtg atgaggctct tctggctgat
     7141 gccaccgcgc tggctggtga ggtcaatgac gcacttgcga aatatgcccg tgccgttcat
     7201 ccggtgcacg gagagatcat cccctacgag actgacggct atttcaacta caatttcatg
     7261 gatgatgcca atgttcccag cctgctcagc atgccatacc ttggaaccat tgaccggagt
     7321 gatccgcttt acctgcgcac acgcaaattt gtgctgagca aaagcaaccc gtactatttt
     7381 tctggcatca gcgggcatgg tgtggggagt ccgcataccg gccgtgaccg catctggccc
     7441 attgccatca tcatgaaagc gctgacagcc tcgaacgacc atgatatcat tacatcactc
     7501 aggatgctca aaaataccca tgcagcaacg ggtttcatgc atgagtcgtt catgaaggac
     7561 gacccctcaa aattcacacg caagtggttt gcatgggcca acaccctgtt cggagaactg
     7621 gttttgcaga catacaagac acagcctgag ctgctgaaag aagtttaaaa cagataacaa
     7681 tgaaagaagt agttgccgga gttgacattg gcggaacgaa caccgtaatt ggttttgttg
     7741 atgctgaagg agtaattcat gccgggggga acctctcgac gcccgattat gctttagccg
     7801 atgattttgt cattgcgctt gacgcgaaac tgaaggagat gatggctgag aacagcggac
     7861 tgaagctgat cggcataggc atcggtgcac ccaatgccaa cttcaataag ggaacgattg
     7921 agctggcgcc caacctgcgg tggaagggga ttattccgct tgtcaggatg accgaagaag
     7981 ccacaggcct caggacccgc atcaccaatg acgccaatgc ggcggcactg ggagaaatga
     8041 ttttcggcgc cgcgaagggt gtgaaggatt tcatcatcct gaccctgggc acgggccttg
     8101 gcagcggctt cgtcgttgac ggcgaggtgg tgtacgggca taccggtttt gccggtgaac
     8161 tgggtcatat gatcgttgtt gagggaggcc gggtgtgcgg atgcgggcgc cgcggctgtc
     8221 ttgaaacata tgcatcagcc acagggctgg tccgtactgc tttgtcactg ctcagcgacc
     8281 gccgtgatga tagcacgctg cgggagctca ggggcagtga gatcacctca cggcggatcg
     8341 ctgaagccgc ggcggccg
//
LOCUS       DAOPBK010000115         8287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7984_length_8287_cov_5.780248, whole genome shotgun sequence.
ACCESSION   DAOPBK010000115 DAOPBK010000000
VERSION     DAOPBK010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7984_length_8287_cov_5.780248"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..78)
                     /locus_tag="PLA17_07590"
     tRNA            complement(4..78)
                     /locus_tag="PLA17_07590"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Asp,seq:gtc)
     gene            complement(458..>1051)
                     /locus_tag="PLA17_07595"
     CDS             complement(458..>1051)
                     /locus_tag="PLA17_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPK85316.1"
                     /translation="HERGAHASVAEPLYCGLVRIIGGKYRGRLIEPPSGFVARPTTDF
                     AREGLMNILNNRYDFSAVSLLDLFGGTGAISFEFASRGTSDIDVVETDRRSCAFISKT
                     LRDLGPEHARVHRIDVRDWLKICHKQFDIIFADPPYNLRWLANIPDMVMASGAVHNST
                     LFILEHPKSADFSNHPLRTDHRKYGNVNFSFFKLPDS"
     assembly_gap    1052..1061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1062..1861)
                     /locus_tag="PLA17_07600"
     CDS             complement(<1062..1861)
                     /locus_tag="PLA17_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HPK85317.1"
                     /translation="MTRSQIYPLELIDETLDINATDNYDLTLELSEEGVSLAVLDLLR
                     GKYVMLRHYPLEGMPEGTVRTYSEILDSDDFLRRRYRKIRIITPSLISTMIPATVYEP
                     ALRDDYFRFNFGVTAEEGIVTNDVPFPDAVVLFSPGREISGIIPSYWHGVTPWHHTKP
                     LLAHVSAACRNMDDCYIHLHLEKSFATVIIAEKRNITFCNSFAISSPQDVAYFLFGVL
                     DRKSIRHDETIYASGLIEPYSETHLALLNFSTGIKFASPLIRQSFSYV"
     gene            complement(1861..2499)
                     /locus_tag="PLA17_07605"
     CDS             complement(1861..2499)
                     /locus_tag="PLA17_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85318.1"
                     /translation="MKKALNIVLTIIIIVLIYFLYESIMEPIRFKKEREFRESAIKEK
                     LIDIRTAQEAFKSVKGYYTGSFDTLITFLKTDSLPLVFKIGSLTDEMIASGITTEREA
                     VRKGLISRDTSYIPVRDSVFKEGYPIDQLRFVPGLENIEFQMSAGRVMTASMVMVNVF
                     EAYVLNDVFLSDLDRQLVVNYNDQKTRVTGGFPGMKVGDVKVPNNNAGNWED"
     gene            2579..3991
                     /locus_tag="PLA17_07610"
     CDS             2579..3991
                     /locus_tag="PLA17_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK85319.1"
                     /translation="MRKENIYNLLTKNLGYLPTQDQSDCMRLLSGFVADNTNDVIFLM
                     TGYAGTGKTSVIASLVATLNTLRQKSVLLAPTGRAAKVLTSYTGMDAYTVHRKIYRQK
                     SATDGVGRFVLDRNFHKETYFIVDEASMIPEESSEGSMFGSGRLLDDLLEYVYTGTDC
                     KLILVGDVAQLPPVGSIVSPALDENVLRGTGFALQMFELRQVIRQSEGSGILMNATAV
                     RQQVSDGDLSVPELVLHGYSDIVRLGGAELIDTLTEAYDRCGTDGVIVVVNSNNLANR
                     YNQGIRNMVFFREEEISSGDLVMVVKNNYYWLNEEESTSFIANGDIAEVKRIRRYEEV
                     YGMRFAEMTLWFRDAELEIDAKVMLDTLMMRTPSLSAEKSRELYFAVLADHPGIKSKR
                     KQYEAVRNDPYYNALQIKFAYAVTCHKSQGGQWERVFIDQGMFNRQEPTIDYLRWFYT
                     ALTRATDKVYLVNFPDSFFR"
     gene            4289..4705
                     /locus_tag="PLA17_07615"
     CDS             4289..4705
                     /locus_tag="PLA17_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPK85320.1"
                     /translation="MKSYRKEFWIETLHRRDYVNITRQVEKCLEESGIKEGLLLVNAM
                     HITASVFINDADHGLHNDFEEWLEKLAPEKPHSQYRHNTFEDNADAHMKRQVMGREVV
                     VAVTNGKLDFGPWEQIFYGEYDGMRRKRVLVKIIGE"
     assembly_gap    4724..4823
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4824..6557)
                     /locus_tag="PLA17_07620"
     CDS             complement(4824..6557)
                     /locus_tag="PLA17_07620"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPK85321.1"
                     /translation="MINIEEIRKKASAWLGEEFDEPTRSEVKHMMENDEKSLINAFYQ
                     DLEFGTGGLRGIMGAGTNRMNRYTLGMATQGLSNYLKKHAANPSSIKVAIAYDCRNNS
                     SYFAKVAADIFTANGFTAYLFESLRPTPVLSYAIRLHGCDAGVIITASHNPKEYNGYK
                     VSWKDGGQVVSPHDRGIINEVRNIRSVKEIAAGGDPSRIVMLGEETDNKYLEEVLKLS
                     LNPEIIRRNADMGIVYTPLHGTGYKMVPQILRMYGFNNIHSVEAQSITDGNFPTLRSP
                     NPEDPEALAMALSLAREKNADLVMATDPDADRLGIAVRKPSGEFVLLNGNQTAALLTW
                     YIVSQKKQKGELKGNEYFVNTIVTSDLLDRIAEQNGVKNYNVLTGFKYFAALIRDLEG
                     KEKYIGGGEESYGYLPGDYVRDKDAVSSCALVAEVAAWAADHGKSMWELLLDIYIEYG
                     LFREKLVNVVREGAEGAAEIKSMMEGYRTNPPASIAGSRVIRINDYLLQVSTDPATGI
                     KTPLDLEKSNVIQFFLANGTKISVRPSGTEPKIKFYFSTSTTMKETSQFPGLWQELED
                     HIDAVIKDMNL"
     gene            complement(6554..7426)
                     /gene="rfbD"
                     /locus_tag="PLA17_07625"
     CDS             complement(6554..7426)
                     /gene="rfbD"
                     /locus_tag="PLA17_07625"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPK85322.1"
                     /translation="MTTILVTGASGQLGSEIRELSTRYSGYEFIFTDIDELDLTDAGA
                     TAAFIAANAPSWIINCAAYTAVDRAEDEEKLATIINAEAVDNLVSTLGGTDCRLIHIS
                     TDYVFDGNSPVPYTEYDTPSPASAYGRSKLAGEKIALSWPGTMVIRTSWLYSSYGNNF
                     VKTILRKAGSSQDISVVFDQAGSPTYAADLANAIMEIISGVIRNKHNFVPGIFNYSNE
                     GVCSWYDLAIEIIREAGSHCSVIPVRSSAWPSKVKRPAYSVLDKTRIKESFNITIPHW
                     RKSLNNCISKINRI"
     gene            complement(7423..7983)
                     /gene="rfbC"
                     /locus_tag="PLA17_07630"
     CDS             complement(7423..7983)
                     /gene="rfbC"
                     /locus_tag="PLA17_07630"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPK85323.1"
                     /translation="MQVHKTEFPGLLIIEPDVFYDSRGYFFESYNKQKYLNNEIVYEP
                     VQDNESRSSRGVIRGLHYQEEPFAQAKLIRVIEGRIYDVALDIRRGSPTFGKWFGLII
                     DSEKKLQFLIPKGFAHGFSVLSEKAIVLYKCDNTYSVAHERGINALDPALGIDWMLRD
                     ETPVISPKDLKQPLFKEISTNFIFRS"
     gene            complement(7986..>8287)
                     /locus_tag="PLA17_07635"
     CDS             complement(7986..>8287)
                     /locus_tag="PLA17_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPK85324.1"
                     /translation="RGELEITDLNRLYLQEGKLEVKLFGRGMAWLDTGTFDSLLQASN
                     FIATLQQRQGLSASCIEEIAYNNGFITRKKLIDLAKPLAKSQYGQYLLKVANENI"
BASE COUNT         1987 a         1936 c         2114 g         2140 t
ORIGIN      
        1 gggcggtgcg gacgggactc gaacccgcga cccccggcgt gacaggccgg tattctaacc
       61 aaactgaact accacaccaa aagcgatgca aaattaatcc aattttctat tcctcaaaat
      121 atttcctgaa aatatattgt ggcgcaacaa taatgacctg tcagcagcgg aaatacctga
      181 tattagatgt aataaatgct ttgaagcaac ccgccgcggt cgcatggtgc cgctggagga
      241 ccttcattag atgtaataaa tgctttgaag caaccggaga tatatcaggt ttactgttct
      301 tgttctgaag ccgtatcatg aagaagtatg cggaggagct attaccatcc tggcaggtga
      361 ttagcgcctt gcgggctgaa gagatgcagt aagatggaac ggagcttcag ggcaggccag
      421 ccaggcaaac tttttgcccg gatagtaaaa ttgcagatta tgagtcaggt agtttgaaga
      481 agctgaagtt tacatttccg tatttacggt ggtcagtccg taaggggtgg ttagaaaaat
      541 cagccgattt cggatgttcg agaatgaaaa gagttgagtt atgcacagct cccgaagcca
      601 ttaccatgtc aggaatattg gcgagccacc tgagattgta gggtgggtca gcaaagatga
      661 tatcaaactg tttatgacag atcttcagcc agtcgcgcac gtcaatgcgg tgaacccttg
      721 catgttcagg accgaggtca cgcagggttt tgctgatgaa tgcgcagctg cgccggtcag
      781 tctccacaac atcaatatca gaggttcccc gggatgcaaa ctcaaagctt attgctccgg
      841 ttcctccgaa gaggtcgagc agggagacag ctgagaagtc atacctgtta ttcaggatat
      901 tcatcagtcc ttcgcgggcg aagtcagtgg tgggcctggc gacgaagcct gatggtggct
      961 caatcagcct ccccctgtat ttgccgccaa ttattcgcac gaggccgcag taaagaggtt
     1021 cagccaccga tgcatgcgca cctcgttcat gnnnnnnnnn natgacatag ctgaagctct
     1081 gcctgatgag tggagaggcg aactttatac cggtcgaaaa gttcaggagc gccaggtgcg
     1141 tttcgctgta gggctcaatc aatccgctgg catagatggt ctcatcatgc cggatgctct
     1201 tcctgtcaag cacgccgaag agaaaatagg ccacatcctg aggtgatgat attgcaaaac
     1261 tgttgcagaa ggtgatattc cgtttttctg caatgattac cgtggcaaaa ctcttttcaa
     1321 ggtggaggtg tatataacag tcatccatgt ttctgcatgc tgccgacacg tgggcgagca
     1381 gcggcttggt atgatgccag ggcgtcacgc cgtgccagta cgaagggata ataccagata
     1441 tttccctgcc cggagaaaag agcacaacag catcgggaaa gggaacatca ttggttacta
     1501 tcccctcttc ggcggttacg ccgaagttga agcggaaata atcatcacgg agagcgggct
     1561 cataaaccgt cgccggaatc attgtggaaa taagcgacgg agtgataatg cggattttcc
     1621 tgtaacgtct cctgaggaaa tcatcagaat caagaatctc actgtaggtc cggacagttc
     1681 cttccggcat cccttcgagg ggataatgcc tgagcatgac atacttgccg cgaagcaggt
     1741 caagcacggc aagagagaca ccctcttcag aaagctcaag cgtaaggtcg taattgtctg
     1801 tcgcgtttat gtcgagggtc tcatcaatga gttccagggg atatatctgc gacctggtca
     1861 tcaatcctcc cagttcccgg cattgttatt gggcacttta acatcaccca ccttcatgcc
     1921 cggaaatcct cccgtgactc tcgttttctg atcattgtag ttgacaacca gttgcctgtc
     1981 gaggtcactg aggaagacat cattgagaac gtaggcctcg aacacgttga ccatcaccat
     2041 ggaggctgtc atcactcttc cggctgacat ctgaaattca atgttctcca atccggggac
     2101 aaacctgagc tgatctatcg ggtagccttc cttgaatacg gaatcacgta cggggatata
     2161 gctggtgtca cggcttataa gacctttcct gacagcttct ctctcggtag ttataccgga
     2221 tgcaatcatt tcatcggtca gcgaacctat cttgaacaca agtggaagcg aatcagtttt
     2281 gaggaaggtt atcagtgtgt caaagctgcc cgtgtaatat ccctttactg atttaaaggc
     2341 ctcctgggca gtcctgatat caatgagttt ctcttttatt gcagattccc tgaattccct
     2401 ttcttttttg aagcggatag gctccataat actttcataa agaaaataga tcagcacgat
     2461 aatgataatg gtgagtacaa tgttgagagc ctttttcata acttatatga tcgatagttg
     2521 ataactttgt gcaaatttaa gaaaaagagc agacgattat caggtttgta gaaataaaat
     2581 gagaaaggaa aacatataca atcttctcac caaaaatctg ggttacctgc ccacgcaaga
     2641 ccagtctgac tgtatgcgtc tgctgtcagg attcgttgcc gacaacacca atgacgtgat
     2701 tttcctcatg accggatatg ccggtaccgg gaagacgagc gtcatcgcct cgctggtagc
     2761 caccctgaac actctccgtc agaaatcggt tcttcttgct ccaaccggaa gggctgcaaa
     2821 ggtgctgacc tcctacacgg gcatggatgc ctacaccgtt caccggaaga tatacaggca
     2881 gaagtcggcc actgacgggg ttggcagatt tgtcctggac cgtaactttc acaaggagac
     2941 ctatttcatt gttgatgaag cctcgatgat ccctgaggag tcatcggaag gctcgatgtt
     3001 tggaagtggc cggttgcttg atgatctttt ggagtatgtg tatacgggaa ctgactgcaa
     3061 gctgatactg gtgggtgatg ttgcgcagtt gccgccggta ggatcaatag tgagtccggc
     3121 actggatgag aatgtgttgc ggggcaccgg attcgcactg cagatgtttg agcttcgcca
     3181 ggtgatcagg cagagtgaag ggtccggcat actgatgaat gccacagccg tccggcagca
     3241 ggtaagtgac ggcgacctgt cagtgccgga gcttgttctt catggataca gtgacatcgt
     3301 tcgcctagga ggagccgagc tcattgatac gctgacagag gcatatgacc gctgtggcac
     3361 tgacggtgta atagtggttg tcaattccaa caacctggcc aacaggtata accagggcat
     3421 ccgtaacatg gtcttcttcc gcgaggagga gataagctcc ggtgaccttg tcatggtggt
     3481 gaaaaataac tactattggc tcaatgagga ggagagtacc tctttcatcg ccaacggcga
     3541 tatcgccgaa gtgaagagga taaggagata cgaggaggtg tacgggatgc ggtttgctga
     3601 gatgacccta tggttccggg atgctgaact ggagattgat gcgaaggtga tgcttgacac
     3661 ccttatgatg cgcaccccgt cactatctgc ggagaagtcg cgcgaactct attttgctgt
     3721 actggcagac catccgggca taaaatcgaa aaggaaacag tatgaggcgg tgaggaacga
     3781 cccctactac aatgcacttc agatcaagtt tgcgtatgcg gtgacctgtc acaagtcgca
     3841 gggcggccag tgggagaggg ttttcattga tcaggggatg tttaaccggc aggagccgac
     3901 aattgattac ctgagatggt tctatacagc cctgaccagg gcgacggaca aggtgtacct
     3961 ggtcaatttc ccggacagtt tcttcaggta ggaaaccggc aacagcagcc atcgggctca
     4021 tcggaataat ttcagttttt cccggatacg caattttccc ggcatggtgg gtgggtgtgg
     4081 cgcatcccaa acatttgtag cgggcaaggg aatagaagcc ctttgccttc tgtaattcag
     4141 aaaagattga aaaaagaatc tccttttgca aaggaatgtc gcctctttat cttctgcccg
     4201 gacagtctgc actgctaaag aggtgaggga atagattaat aatagaattc tctgtatata
     4261 tttgtacaaa agaatcaatt tcgccattat gaaatcttac cgtaaggaat tctggatcga
     4321 gacactgcat cgcagggatt atgtcaatat tacccgccag gttgagaagt gcctggaaga
     4381 gagcggaatc aaggaaggct tactacttgt taatgccatg catataacgg caagtgtttt
     4441 catcaatgat gctgaccacg ggctgcataa cgacttcgag gagtggctgg agaagctggc
     4501 gccggagaag cctcactctc aataccggca caacactttt gaggacaacg ctgatgccca
     4561 catgaagcgc caggttatgg ggcgtgaggt agtggtcgca gtaaccaacg gcaaactcga
     4621 cttcggtccg tgggagcaga tcttctacgg cgagtatgac ggaatgcgga ggaagcgggt
     4681 gctggtgaaa ataatcgggg aatagactgg aaatagctgg caannnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnctacaga ttcatatcct taatgacagc atcaatatga
     4861 tcttccagtt cctgccagag gcccgggaac tgcgaggtct ccttcatcgt ggtgctggtg
     4921 ctgaagtaga actttatctt tggttcggta ccggaggggc gcacggatat cttcgtgccg
     4981 ttggcaagga agaactggat cacatttgac ttttccaggt caaggggtgt tttaatgccg
     5041 gtggcggggt cggttgaaac ctgcaacagg taatcattta tcctgatcac cctgcttcct
     5101 gcaattgaag ccggcggatt ggtgcggtat ccttccatca tgctctttat ctcggcagct
     5161 ccctcagccc cttccctgac cacgttgacc agtttttccc tgaagaggcc gtactcgatg
     5221 tagatgtcaa gcagcagttc ccacatagat ttgccatgat cggcagccca tgccgctacc
     5281 tcagcgacga gggcgcatga tgaaacagcg tccttgtccc tgacataatc gcccggcagg
     5341 tagccgtagc tctcttcacc tccgccaatg tatttctctt taccttccag gtcacgtatg
     5401 agggctgcga aatatttgaa cccggtgaga acattgtaat tcttcacgcc gttctgttca
     5461 gctatcctgt caagcaggtc agacgtaact atagtattga caaagtattc attgcctttc
     5521 agctcacctt tctgtttctt ttgcgagacg atgtaccagg tcagcaatgc tgctgtctgg
     5581 ttcccgttca gcagcacgaa ctcaccggaa ggttttctta ctgctattcc caggcggtct
     5641 gcatcagggt ctgtagccat caccaggtcg gcattcttct cgcgcgccag gcttagggcc
     5701 atggccagtg cctcagggtc ctccggattg ggagaacgca gggtggggaa gttgccatcg
     5761 gtaatacttt gggcttcaac agaatggata ttattgaagc catacattct cagtatctgc
     5821 ggaaccattt tgtatcccgt accgtgaagt ggagtataga caattcccat gtcagcattt
     5881 cgcctgatta tttccggatt gaggctcagt ttcagcacct cttccagata cttgttgtct
     5941 gtctcttcgc caagcatcac aatccgggaa ggatctccgc cagcggctat ctcctttact
     6001 gacctgatgt tccggacctc attgatgatt cccctgtcat ggggagaaac cacctggccg
     6061 ccatccttcc atgaaacttt ataaccgtta tattctttag ggttgtgcga ggcggtgatg
     6121 atgacgccgg cgtcacaacc gtgcagtctg atggcgtagg agagcaccgg tgttggcctc
     6181 agagactcaa acaagtatgc ggtaaaacca ttggcagtga agatgtcagc ggccactttg
     6241 gcgaaatagc tgctgttatt ccgacagtca tatgctattg ccaccttgat ggatgacgga
     6301 ttggctgcat gttttttcag atagtttgag agtccctgtg ttgccatccc cagggtgtag
     6361 cggttcatcc tgttggtgcc ggcgcccata atgccgcgca ggccgccggt gccaaactca
     6421 aggtcctgat agaatgcgtt gataagactt ttttcgtcgt tctccatcat atgtttcacc
     6481 tcgctgcggg ttggttcgtc aaactcttcc ccgagccatg ccgatgcttt cttcctgatt
     6541 tcttctatgt tgatcatatc ctgtttattt ttgagataca gttgttaagg cttttacgcc
     6601 aatgtggtat tgtgatgttg aaagactcct ttattcttgt cttgtcaagc acgctgtatg
     6661 ccggcctttt cacctttgaa ggccatgctg agctcctgac gggaatgacg gaacagtgtg
     6721 accctgcctc cctgatgatc tcgatggcaa ggtcatacca gctgcagacg ccctcattcg
     6781 aatagttgaa gattcccggg acgaagttat gcttattacg gatcacgccg ctgataattt
     6841 ccataatggc atttgcaaga tcggcagcat aggttggtga acccgcctgg tcaaagacca
     6901 cgctgatatc ctgtgatgat ccggccttgc ggaggatagt ctttacgaaa ttatttccgt
     6961 aggaggagta tagccatgat gtcctgatga ccattgtgcc gggccaggac aaggcaatct
     7021 tttctccggc cagtttactg cgtccgtaag ctgatgccgg cgaaggtgtg tcatactcgg
     7081 tatatggcac cgggctgttg ccgtcaaaga cataatcagt agagatgtga atcagcctgc
     7141 agtcggttcc cccgagggtg ctgaccagat tatcgaccgc ttcggcgtta atgatggtcg
     7201 caagcttttc ctcgtcctca gccctgtcaa cagctgtata tgctgcgcag tttatgatcc
     7261 atgaaggtgc atttgcagca atgaatgcag ccgtcgctcc ggcatcggtt aaatcaagct
     7321 cgtcgatatc agtgaatatg aattcatagc cgctgtaacg ggttgacagt tccctgatct
     7381 cactgcccag ttgtccggat gctccggtca ccagtatagt tgtcatgatc tgaagatgaa
     7441 gttagttgaa atctctttga agagaggctg tttgaggtct ttggggctta tgaccggagt
     7501 ttcgtccctg agcatccagt cgattccgag tgcagggtcc agggcattga ttcccctttc
     7561 atgtgcaact gaataggtgt tgtcacactt gtaaagcact attgcctttt cactgagcac
     7621 tgagaaacca tgggcaaatc ctttgggaat aaggaactgc agttttttct cagagtcaat
     7681 tatcagcccg aaccacttcc cgaaggttgg ggaacccctg cggatatcca gtgcaacgtc
     7741 atagatgcgc ccctcaatca cccttatcag ctttgcctgt gcaaacggct cctcctggta
     7801 gtggaggccc cggataacac ccctgctgga tcttgattcg ttatcctgaa caggttcgta
     7861 gactatttcg ttatttaagt atttctgctt gttatagctt tcaaagaaat aacctcggga
     7921 gtcataaaaa acatccggtt caattataag aagccccggg aattcagttt tgtgtacctg
     7981 cattttcaga tattttcgtt tgccactttc agaagatact gtccgtactg gcttttggcc
     8041 agcggtttgg ccagatcgat cagttttttc cgggttatga aaccgttatt gtaggcgatt
     8101 tcctcgatgc aggaagcgct gagtccctgc ctttgctgta atgttgcaat gaagttggat
     8161 gcctggagga ggctgtcaaa tgttcctgta tcaagccagg ccattccgcg accgaaaagt
     8221 tttacttcca gttttccttc ctgcaggtag agcctgttca gatcagttat ctccagctct
     8281 ccacggg
//
LOCUS       DAOPBK010000116         8279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7989_length_8279_cov_4.432393, whole genome shotgun sequence.
ACCESSION   DAOPBK010000116 DAOPBK010000000
VERSION     DAOPBK010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7989_length_8279_cov_4.432393"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLA17_07640"
     CDS             complement(<1..588)
                     /locus_tag="PLA17_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPK85325.1"
                     /translation="MDAYFSQRVNDILGYSKEEAIRLGNSHISPEHLFLGILREGEGV
                     AVDILLNNGVDLLMLKGSIEKSIKADRPVAMADNDILPLLKSTERVLRLVHIEAKALK
                     TRVIDTEHLLMAILRDESNLVTRFLTELDIDYSLVKKAVEASNPAAQTDYPRDEDDEG
                     IFSNPGPSGRQQQGGQQAQQRSSSETPVLDNFGIDL"
     gene            832..3330
                     /gene="gyrA"
                     /locus_tag="PLA17_07645"
     CDS             832..3330
                     /gene="gyrA"
                     /locus_tag="PLA17_07645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPK85326.1"
                     /translation="MSDHARIVKVNIEEEMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMSELGVLSNRPFKKSARIVGEVLGKYHPHGDTSVYDAMVRMAQDWSLRYPLV
                     DGQGNFGSIDGDSPAAMRYTEAKLKRIAEETLSDIEKETVDFQPNFDDTLQEPTVLPT
                     RIPTLLVNGAAGIAVGMATNMPPHNLSEAVDAIVAYIDDPEITTEGLMHHIKGPDFPT
                     GGIIYGEQGIREAYETGRGRIVVRAKTEVELTHSGRECIVITEIPYMVNKADMIRKIA
                     DLINDKKLEGISYINDESDRAGMRIVIILKKEMPANIVLNRLYKQTQLQTSFNVNNIA
                     LVKGRPRTLTLKEIIGNFVSHRHDIVVRRTKYDLDQAEKRAHILEGLIIASDNIDEVI
                     AIIRSSQNTDIARERLMERFGLTDIQARAIVDMRLRQLTGLEQDKLHAEYEELKRLID
                     HLNQVLADERLQMQIIKDELLEVKDKYGDKRRTQIVPAAGEFNPEDFYADDEMVITIS
                     HMGYIKRTPLTEFRRQNRGGTGKRGSTTREEDFIEHMYVATMHNTMLFFTSKGKCYWQ
                     KVYDLPEGTRNSKGRAIQNVINIEPDDSVRAFINVKTLDDSDYINNNYIVLCTCKGVI
                     KKTSLEAYSRPRVNGINAITVREGDELLEARMTNGSHEIMLAVRSGRAVRFKESTVRP
                     MGRTASGVRGITLSGDDDVVVGMITVENEAADVLVVSENGFGKRSAISDYRLTNRGGK
                     GVKTINITDKTGKLIALKDVSDQYDLLIITQNGNILRTPVNELRVMGRATQGVKLIDI
                     KENDSIASVAYVEVSEDDNETDNEIGEESAESAE"
     gene            3404..4549
                     /locus_tag="PLA17_07650"
     CDS             3404..4549
                     /locus_tag="PLA17_07650"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85327.1"
                     /translation="MKKITLLLLALIISAGAMAQKGKITAAETFIRDGAFDKAKEAIE
                     TAIAHPKSMGLAKTYYVKGKLCQAAFDSGDPKFADLYPDILMEAYNNFEKAIQLDPKM
                     KNTIIRDNAYALLGNSFLNDAINKFNDRNFKGAMESFERNVTISASDLYVGMVDTLVI
                     FNAGLAAYNGEMYPEAIAHFRTCTESGTEDTKPYIFMSDCYMKMNDTVRAEAVLMEGY
                     KAYPDTLDIILQATQFYLDSDNSPKAFEFVHKAMEMDPDNHILYLVEGSLYMKLEDYE
                     KAIESLKISLEKNPTQFESNYNIGLCYVSITNNMLNEANMIDDNREYEIARDKAFEEM
                     KKAIPYLLEAEKVNPTSVPTLEFLREIYLKLKMMPEFEEYKAKVENM"
     gene            5095..5760
                     /locus_tag="PLA17_07655"
     CDS             5095..5760
                     /locus_tag="PLA17_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPK85328.1"
                     /translation="MITIDRSTLKFLADLRRHNDRLWFAENRSRYEAARENLYLFVQA
                     VIDDIVKFDPIFKGLEAKSCIYRINRDIRFTIDKTIYKTNFGAFMVRGGKKNGDRFPG
                     YYLHIEPGLSFISGGSYIPPAPWLKAIRERIAMEGDNLVKILNSREFRKYFNGLEGEK
                     LKLPPRGFSKDHPHIELIKMKSFLTEKPLTDSEVVDPGFFEVVTGAFRAMKPLNDFLT
                     TGR"
     gene            5852..6385
                     /locus_tag="PLA17_07660"
     CDS             5852..6385
                     /locus_tag="PLA17_07660"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HPK85329.1"
                     /translation="MKKLLFTLLLAAFILPFAAAQGKLEVGKKAPEWALPDASKQEFT
                     MNSWPGKVLQINYVDPDNEDLNDTFNDAVEKAIDIDKIIDQNAYKGFGIVDLQSTRLP
                     DGLVRAIAGRKAKRYNTTILFDSKGVLHESWGLPKDSYSVVIVDKNRIVRGIYYGKIA
                     DADIPGIIEMIVKLTKE"
     gene            complement(6562..7035)
                     /locus_tag="PLA17_07665"
     CDS             complement(6562..7035)
                     /locus_tag="PLA17_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85330.1"
                     /translation="MSVKDYNPAMHTAEHILNSVMDKMYHCGRSFNSHIESKKSKCDY
                     RIRKALSDKEIRAVERTVNEIIKLDIPVGEELVSRREADEKYFTGKLPEDAGETIRII
                     TIGNFDACPCIGQHVKSTSEIGTFSITSADFNEGVLRIRFRIRREEPQSGLRLRA"
     gene            complement(7084..>8279)
                     /locus_tag="PLA17_07670"
     CDS             complement(7084..>8279)
                     /locus_tag="PLA17_07670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPK85331.1"
                     /translation="RGLLAVQTTPDPVTGKSVSIFLNDLKYYEMIPENPESLWQMILL
                     RSPVYDKLLKSGYSYETRSAMEDMMKEYLSRAEQNNSFYIDSYLEARLYAILRKVYPV
                     RHTDKRPGIVSLRILAEIAPDAWVGPDGTLVITTGMIAALDNETEMMALMAQEVAHFA
                     LDHHLSNYSAALALEINPALGNLIRNTRQQEMEADNCATSVLSYYGKSFSFLGSMLRN
                     VIDYGELMGSFYLGSFPDAKSRADKYKDAIIDKSADYDKLVAPVISYNAFMAYSQSHY
                     LLCRRLLERNIASGSATADDIVLLSQAMMNLSGSDHEDLEALALVRSVTESLQEAPPG
                     AFRQEALLLMRLGRRGETETALDRYQEALEREEMKYSSRPGDWSEVLSYLASEKEWVA
                     RTRTR"
BASE COUNT         2301 a         1859 c         2003 g         2116 t
ORIGIN      
        1 aaggtctatg ccgaagttat caagcaccgg agtctcggac gaactcctct gctgtgcctg
       61 ctgtcctcct tgctgctgtc ttccggatgg tccgggattg gaaaagatcc cttcatcgtc
      121 ctcatcacgg ggataatctg tctgggctgc aggattactg gcttcgactg cctttttgac
      181 aagagaataa tctatgtcaa gttctgtcag gaaccgggtg acaaggttac tctcgtcgcg
      241 caggatggcc atcaaaaggt gttcagtatc tataaccctg gtcttcaagg ccttggcttc
      301 tatatgaaca agcctgagaa cccgttcggt gcttttcagc agtggcagga tgtcattgtc
      361 tgccatcgcc accggtctgt cagccttgat actcttctca atggatccct tgagcatcag
      421 caggtccact ccgttgttca gcagtatatc taccgcaact ccttcgccct cgcggagtat
      481 gccaaggaag aggtgttccg ggcttatatg gctgttgcca agcctgattg cctcctcttt
      541 gctgtaacca aggatatcat tgactctctg tgagaaatat gcgtccatta ctttttcgtt
      601 acaggtttca tgaaattgtg tcagttaaac tgccattaat acataaaacg ccagattgca
      661 aagttaattc tattgtaaga atgtatcggc aagaattgtg cccaattttt aacatcagat
      721 gttaacaaat agaggcttta aaagggtaaa aaatgagcca ttttgattat tttcgtggat
      781 taattccaaa tgataaaaat acatttataa cttattaatt atcaatctga tatgtcagat
      841 catgcaagaa tagtaaaagt taatatcgag gaggagatga agtcggctta catcgattat
      901 tcgatgtcag tcattgtgtc aagggcatta cctgatgtgc gtgacggctt gaaaccggtg
      961 cacaggaggg ttctctttgg tatgtcagag cttggagtac tgtcaaacag gccattcaag
     1021 aaatcagcaa ggatagtggg ggaggttctg ggtaagtatc acccgcatgg agacacgtcg
     1081 gtttatgatg ccatggtaag gatggctcag gactggtcac tacggtaccc gctcgttgac
     1141 gggcagggaa acttcggatc aatagacggt gacagtcctg ccgcaatgcg atatacagag
     1201 gctaagctca agcggatagc agaagagact ctctctgaca ttgaaaagga gactgttgac
     1261 ttccagccca attttgatga cacgctgcag gagcctacag tgttgcccac caggatacca
     1321 acccttctgg tcaatggagc agcaggtatt gccgtgggta tggcaaccaa catgcctccg
     1381 cacaacctct ctgaagcagt tgatgccatt gtagcttaca ttgatgaccc ggaaatcacc
     1441 accgaggggc tgatgcacca tatcaagggg cctgactttc cgacaggggg tataatctat
     1501 ggcgagcagg ggataaggga ggcttatgaa accggcaggg gaaggatagt tgtcagggcc
     1561 aaaacggagg ttgaactgac ccattcggga agagagtgta tagtcatcac cgaaattccc
     1621 tacatggtca acaaggctga tatgatcagg aagatagctg accttatcaa cgataagaaa
     1681 ctggaaggaa tctcctacat caatgacgag tctgacagag cagggatgcg gattgtgata
     1741 attctcaaga aggagatgcc tgccaacatt gtcctgaacc gtctctacaa acaaacacag
     1801 cttcagacat cattcaatgt caataacata gcgcttgtca agggccgtcc caggaccctt
     1861 accctgaagg agataatagg caatttcgtg agtcacaggc atgatatcgt tgttcgcagg
     1921 acgaaatatg atcttgacca ggctgagaag agagctcata tacttgaagg tctgatcatt
     1981 gccagtgaca atatcgatga ggtaatagct atcatcaggt catcgcagaa tactgacatt
     2041 gctcgtgaac gtctgatgga gaggttcggg cttactgata ttcaggcaag ggccatcgtg
     2101 gatatgcgcc tgaggcagct cacagggctg gaacaggata agctgcatgc tgaatacgag
     2161 gagcttaaaa gactcattga ccacctgaat caggttcttg cagacgagag gctccagatg
     2221 cagattatca aggatgagct tctcgaggtt aaggacaaat acggagacaa acgacggact
     2281 cagattgttc ccgccgcagg cgaattcaat cctgaagatt tctatgctga tgatgagatg
     2341 gttattacca tttcacatat gggctacatc aagcggacgc ccctgaccga gttccgccgt
     2401 cagaacaggg gagggacagg caagaggggg agcaccaccc gggaagagga tttcattgag
     2461 catatgtatg tggcaacaat gcataacacc atgctcttct ttaccagcaa gggcaagtgc
     2521 tactggcaga aggtctatga cctgcccgag ggtacgcgca acagcaaggg aagggcaatt
     2581 cagaatgtta tcaacattga gcctgatgac agcgtcagag cattcatcaa tgtaaagact
     2641 cttgatgaca gtgactatat caacaataac tacattgtcc tctgcacctg caagggagtc
     2701 atcaagaaga cgtcgctcga agcctattcc cgccccaggg taaacgggat caatgccatc
     2761 actgtgaggg aaggggatga gcttcttgaa gccaggatga ccaacggcag ccatgagata
     2821 atgcttgcag tgagatcagg ccgtgccgtc aggttcaaag aatccaccgt caggccaatg
     2881 ggccggactg catcaggtgt gcgcgggata accctctcag gcgatgacga tgttgtagtg
     2941 ggcatgatta ccgttgagaa cgaagctgca gatgtactgg tagtctcgga gaacggcttc
     3001 ggaaagaggt cggccataag tgattaccgc ctcacgaacc gcggcgggaa aggggtgaag
     3061 acaataaaca tcactgacaa gaccggaaaa ctgatagccc tcaaggatgt gagcgaccag
     3121 tatgatctgc tgattattac gcagaacggt aacatcctga ggacgccggt gaacgaactg
     3181 agagtgatgg gcagggcaac tcagggtgtg aaactcatcg atatcaagga aaatgactca
     3241 attgcctcag tagcttatgt agaagtaagt gaggatgata acgagactga caacgaaatc
     3301 ggggaagagt cggccgagag tgctgagtaa tttggcaaat tatttgaaga atagattcta
     3361 aagttttatt tttggaccaa tattaaaagc aaattatcca gacatgaaga agattacatt
     3421 gttactactg gcactgatca tttcagcagg agccatggct cagaagggaa agattaccgc
     3481 tgccgaaact tttatcaggg atggtgcttt cgacaaggct aaggaagcca ttgagacagc
     3541 catcgctcac ccgaagtcaa tgggccttgc aaagacttac tacgttaagg gaaagctttg
     3601 ccaggctgcg tttgattcag gcgatccgaa gtttgccgat ctttatcctg atatcctcat
     3661 ggaagcctac aataacttcg agaaagccat tcagcttgat cccaagatga agaacaccat
     3721 tatcagggac aacgcatatg cgctccttgg caactcattc ctgaatgacg caatcaacaa
     3781 gttcaatgac agaaacttca agggagccat ggaatcgttt gaaaggaatg tgacaatttc
     3841 tgccagcgat ctctatgtag gcatggtaga cacgctggtt atcttcaatg ccggtctggc
     3901 agcctacaac ggtgagatgt atcctgaagc aattgcacat ttccgtacct gcaccgagtc
     3961 gggtaccgag gatacgaagc cttatatatt catgagcgac tgctacatga agatgaatga
     4021 taccgtcaga gccgaagctg ttctgatgga agggtacaaa gcatatcctg acacgctgga
     4081 tatcattctc caggcaaccc agttctatct tgactccgac aattcaccaa aggcatttga
     4141 attcgttcac aaggccatgg agatggatcc ggataatcat atcctttacc tggttgaagg
     4201 aagcctctat atgaagcttg aagactacga aaaagctatt gagagcctga agatatcact
     4261 ggaaaagaac ccgacacagt ttgaaagcaa ctacaacatc ggtctgtgct acgtttcaat
     4321 tacaaacaat atgctgaacg aggccaatat gattgatgac aacagggagt atgagattgc
     4381 acgggacaag gcatttgaag aaatgaagaa ggcaattcct tacttgctgg aagcagaaaa
     4441 ggtaaatccg acttcagttc ctacattgga gttccttcgt gagatctatc tcaagcttaa
     4501 gatgatgcct gaattcgagg aatacaaggc aaaggttgag aacatgtaat aaatcgttgt
     4561 atgaacaata tggagaaggc acacgttgat gtgccttctt tttttgcctt tactgtacgt
     4621 ggttggcata atatcagaat tttccagggt tgcagcgggg gcaggggggc tattttgcca
     4681 ccggcagccg gattttgcca ttttgtgctg gtatttctgc ctctatttaa cataatatta
     4741 attataggca aatataaagc aaattgcgtc cacgcctctg cgtggttggc ataatatcag
     4801 atatgggccg caaggaccat atcggcaccg atattatgtc aaagcaattt gcggccacac
     4861 tttagcggcg ccaagccaaa gcaatttgcg gccacacttt agcgtcgcca agccaaagca
     4921 atttgcggca ttatttgcag atggtcagta aaaattgcgt aaacactttt gcacggttgg
     4981 cattgtatca tctatggata tcaaggagta taccggcatc gtttttgatt ctgtctgtag
     5041 tagcctgggg aaaaaagtaa ttacataact ttgtcagatt aaccggataa taatatgatc
     5101 actattgacc gttccactct taagttcctt gctgacctgc gccggcataa tgacaggctg
     5161 tggtttgcag aaaaccgttc ccgctatgaa gcagcccgcg aaaatttgta tctctttgta
     5221 caggctgtca ttgatgatat agttaaattc gatccgattt tcaaagggct tgaggcaaaa
     5281 agctgtattt acaggatcaa ccgtgacatc cggttcacaa ttgacaagac catatataag
     5341 actaatttcg gagcattcat ggtccgtggc ggaaaaaaga acggcgacag gtttcccggt
     5401 tattaccttc atatagaacc aggcctgtcg tttatttcag gaggatcata catacctcct
     5461 gcgccctggc tgaaagcaat ccgcgagaga attgccatgg aaggcgacaa ccttgtgaag
     5521 attctgaaca gcagggaatt caggaagtat ttcaatggac ttgagggaga aaaactgaaa
     5581 ctgcctcccc gcggattctc aaaagatcat cctcatatcg agctgattaa gatgaaaagc
     5641 tttctgactg aaaaaccact cactgacagt gaggtggtag atcccggttt tttcgaagtg
     5701 gttacaggtg ctttcagggc aatgaaaccg ctgaacgact tccttacaac gggcagataa
     5761 tatgtaccac gtttgcattc acatttttcg tcataatgtg catgaacagt aaaaaatatt
     5821 aactttcagc aaaatttaag aaactgtgaa aatgaaaaaa ttgttgttta ctcttctcct
     5881 tgcagctttt atactgccgt ttgctgcagc ccagggcaag cttgaggttg ggaaaaaagc
     5941 tccggaatgg gctctgcccg atgcttccaa acaggaattc accatgaact cctggccggg
     6001 taaagttctt cagataaact acgttgatcc tgataatgag gaccttaatg atacattcaa
     6061 tgatgccgtt gaaaaggcca ttgacattga caagattatt gaccagaatg cctacaaggg
     6121 attcgggata gttgacctcc agtcgaccag gcttccagac ggtctggtac gtgcaattgc
     6181 cggcaggaag gcaaaaaggt acaacactac tattctgttt gattccaaag gagttctgca
     6241 tgagtcatgg ggcttgccca aagacagcta ttcggttgtg attgttgaca agaaccgcat
     6301 tgtcaggggt atatactacg gcaagattgc cgatgctgac atcccgggca tcattgaaat
     6361 gattgtcaaa ctcacaaaag aataggaact accttcatga cagatcagaa gccgtctcat
     6421 aagataattg aggcggcttt tattattcca gagagcttct ttatatcagc tttacggtta
     6481 catctcagat ccgcattcag aaacgtgagg cggcttttac gattccaatt agagctcttt
     6541 ataccagctc tgttatacct gtcaggcccg caaccggagg ccggattgag gctcttcccg
     6601 gcggatcctg aagcggattc tcaggacacc ttcattgaaa tcagccgatg ttattgaaaa
     6661 tgtgccaatt tcagatgtgc ttttcacatg ttgcccgatg cacgggcatg catcgaaatt
     6721 cccgatagtg atgatccgga ttgtttctcc tgcatcttca ggaagcttcc ccgtaaagta
     6781 cttttcgtca gcctccctcc tgctgacaag ctcctctccc accggtatat caagcttaat
     6841 tatctcattt actgtccttt caactgcccg aatctcctta tcagacaatg ctttcctgat
     6901 gcgataatcg catttgctct ttttgctctc aatgtgactg ttgaatgatc tcccgcaatg
     6961 gtacatcttg tccatcaccg agttcaggat atgctcggca gtatgcatag caggattata
     7021 atctttaact gacatgatga atgtatatct ggaatattaa aacgtcagtg cggggtttat
     7081 attttacctc gttcttgttc tggccaccca ctccttctcc gatgccaggt agctgagcac
     7141 ttcagaccag tcgccgggcc tcgaactgta tttcatctct tccctttcca gtgcctcctg
     7201 gtatctgtca agagctgtct cagtttcacc ccttcggcca agtctcatta aaagaagggc
     7261 ctcctgcctg aatgctccgg gaggagcctc ctgcaggctc tctgtcacag agcggaccag
     7321 tgccagtgcc tccagatctt catgatctga gcctgacagg ttcatcatag cctgtgagag
     7381 caacacaata tcatcggcag tagctgaccc tgaagcaatg ttccgttcga gcaggcggcg
     7441 acagagcagg taatgtgact ggctgtatgc catgaaggca ttatatgata taacaggtgc
     7501 aacaagttta tcataatcag cggatttgtc aataatcgca tccttgtatt tatctgccct
     7561 ggatttggca tcaggaaatg atccgaggta aaaactgccc atcagctctc cgtaatcaat
     7621 tacatttcgc agcattgaac ccaggaaaga gaaactcttg ccgtaatatg agagtaccga
     7681 agttgcacaa ttatcagcct ccatttcctg ttgcctggta ttcctgatta agtttcccag
     7741 tgcggggttt atttctaacg caagagcagc agagtaattg gaaaggtgat ggtcgagggc
     7801 aaaatgggca acttcctggg ccatcagagc catcatctcg gtctcattgt ccagggctgc
     7861 aatcattccg gtggtaatta ccagggtgcc atcagggccc acccatgcat caggagcaat
     7921 ctccgccagg attcggaggc tcactattcc cggacgcttg tcggtatgcc tgaccggata
     7981 gaccttacgc aggatggcat acagcctggc ttccaggtaa ctgtcaatat aaaatgaatt
     8041 gttctgttct gcccttgaca ggtactcctt catcatatcc tccatcgcag agcgggtctc
     8101 atagctgtaa cccgacttca ggagcttgtc atacacgggt gatctgagaa gtatcatctg
     8161 ccacagtgat tccgggtttt caggaatcat ctcatagtat ttgaggtcgt taaggaaaat
     8221 actcacgctc tttccggtaa ccgggtcagg ggtggtctga accgcaagga gtccgcgtg
//
LOCUS       DAOPBK010000117         8267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_7997_length_8267_cov_4.880662, whole genome shotgun sequence.
ACCESSION   DAOPBK010000117 DAOPBK010000000
VERSION     DAOPBK010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7997_length_8267_cov_4.880662"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLA17_07675"
     CDS             complement(<1..501)
                     /locus_tag="PLA17_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPK85332.1"
                     /translation="MSFIDNDLKYRVKDISLAGFGRKEIEIAEKEMPGLLAVRRKYSG
                     SKPLRGARITGSLHMTIQTAVLIETLRELGADVRWASCNIFSTQDHAAAAIAAAGIPV
                     FAWKGETAEEYWWCTAQALTFPGGKGPNLIVDDGGDASLLVHLGYKAEKESSVLDAEP
                     ASREEAI"
     gene            complement(552..1490)
                     /locus_tag="PLA17_07680"
     CDS             complement(552..1490)
                     /locus_tag="PLA17_07680"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPK85333.1"
                     /translation="MKVYHGYTGISFNNPVITIGVFDGVHLGHRMLLRRVKGEAAKDG
                     ADAVAVTFDPHPRMILSSDPGHLSFLTDLEERTELMRETGIDYLVVIPFTAELSRMTA
                     PEFISSILCRHLGVCHLIIGYDHHFGRRREGDENTIIECARRMAFRVTREEAYMVEGE
                     AVSSSLIRKALGEGNIEKAASMLGYDYFIRGRVVSGRKIGRGMGFPTANIATLSAYKL
                     IPRSGVYAVEVIVEGVPDRHMAMLNIGHRPTITGGDGLCTIEAHLIDFDGDLYGNEVT
                     VTFRHRLRDEMKFGSVDELSARLTLDREMTIALLGR"
     gene            1560..3578
                     /locus_tag="PLA17_07685"
     CDS             1560..3578
                     /locus_tag="PLA17_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HPK85334.1"
                     /translation="MTERFYKADPWLDPFREIIEARLAYISYREKLFTGGRSMTDYAN
                     GHHWYGLHRTPEGWVFREHAPNATSIILTGTFSEWKEDGRYALSRTAEGDWVGEFDAG
                     MLSHGDLYKMIVKWKGGQGERIPAYATRAVQDEVTKIFSAQVWNPENEYVMQHKAPPP
                     PPSLLVYEAHVGMATEEERTGTYREFTRHVLPRVKSLGYNTVQLMAIQEHPYYGSFGY
                     HVSSLFAPSSRFGTPDDLRELIDTAHSMGLRVTMDLVHSHAVANILEGLGLLDGDPGL
                     YFHTGSRRKHVAWDSLCYDYGKPEVNHFLLSNCKYWLEEFHFDGFRFDGITSMLYYDH
                     GLESDFVSYKQYFDGNQDKDAIVYITLANKLIHEFNQDATTVAEDMSGMPGLAASVAS
                     GGMGFDYRLAMGVPDIWIKMVKDIPDEEWDLGYLLHELTQHRTEEKVISYCESHDQAL
                     VGDKTLIFRLADKEMYTSMSIFTPSLIVDRAMALHKMIRLFTFMTAGGGYLTFMGNEF
                     GHPEWIDFPREGNGWSYKYARRQWSLADDELLKYRFLNEFDRAMIETGKRHGLPRLQL
                     TESLLVNNADKIIAFRRGDLVTVMNFNPTISFTGYGIPVRGKFRIILDTDDPMFGGQG
                     RIDTNQTYFAQPDKGRYGVTAMHHLRLYLPARTAVVLLQERVKTIMDL"
     gene            complement(3581..7804)
                     /gene="glgP"
                     /locus_tag="PLA17_07690"
     CDS             complement(3581..7804)
                     /gene="glgP"
                     /locus_tag="PLA17_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPK85335.1"
                     /translation="MKADFLFETSWEVCNKVGGIYTVISTKALNVTYEFEDNYILIGP
                     DVWRDDKSNPEFTPDDTMFQSWKTVAAQEGLIVKTGRWNIRGNPRVMLVDFTPYFGQQ
                     NEIFARFWETYKLDSISGQWDYIEPALFGYAAGKIIESFTNFYREYPRVIAHFHEWMT
                     GTGVLYLEKHAPWVATAFTTHATVLGRSIAGNNRQLYSRLNEYNPVQTAHEFNVTSKQ
                     SLEKITAQEADVFTSVSEITGRECEAFLGKKPDVITPNGFEDTFVPEPENFDAARNAA
                     RDRLLSVAKALIGCDLPDDTLLVSTSGRYEYRNKGIDLFLSALGQVNRKSNSGRQCVA
                     FILVPAYHKGPRQDLATALANGTMVTEGDKYLTHGLHYVNDDPVMNQLIREGIHNIAS
                     ERVKVIFVPSYLNGNDGIFNMKYYDLLIGFDLTLYPSYYEPWGYTPLESLMFHVPTVT
                     TNLAGFGMWVDTYFKEPGNGCLVLNRTDDNDNEVIVSMEEFIRKFMQMNPEEAAAARG
                     KAWEISRIANWNTLFSYYINAYRYALEKSEKRRDQPRDYTRILEATEVQVRKPFQAPV
                     WKDIYVQSELPVRIAYLKELSSNLWWSWNSDAEFLFRRMDPTLWEEVGHNPKRLLEAI
                     DYKRLVVLSDDEVFLHDADRIYKEFTEYLGRPDNEELPSVAYFSMEFGIHPNLKIYSG
                     GLGVLAGDYLKEASDSNVNITGIGLLYRYGYFRQKLGPRGEQQAIYEAEDFSQIPVEP
                     VKESNGNHLTIQLTWPGRIVKARVWLAPVGKVKLYLLDTDFEDNTPEDRAITHYLYGG
                     DSENRLKQELILGIGGMRALLAIGIKPDVYHSNEGHSAFIGFERMRHLIEDEHLTFAE
                     SMEIIRASTLFTTHTPVPAGHDAFEEDLLRKYISHYHTRLNITWDQLMALGRCQDDHE
                     RKFNMSFLATRFSQEMNGVSQLHGHVSRGLFSKLWPGYLRDELYIGHVTNGVHYSTWV
                     APEWEQVYEKLTGTRHFDLSDRKQWAKIHKLEDEKVYEVKMKLKKRLFDNIRKRLQSD
                     MLERHVSPRTLMNISSHLNEKALTIAFARRFATYKRANLLFRDLDRLARIVNNPERPV
                     QFIYAGKAHPHDGGGQELIRKINEISQMPQFAGKIIFLENYDMDMARYLVRGADIWLN
                     TPTRPLEASGTSGEKAVMNGTIHFSVLDGWWVEGYRAFAGWALPKKRTFASQELQNDI
                     DAETIYNMLEYEIVPAYYSKDESGIPREWVSYIKNTMVKVAPEFTMRRMLNDYLEKYY
                     KKLWERSRRMAADNYALARELAGWKSYISKVWDEIEVVKFDIKDLDKNVFRSGYKYST
                     EIILDIKDIPVENVGVELVVTRQLKNGEHAFFSSHEFEYVNRKKGRVIYSVEFQPEIA
                     GTFFYGIRIYARHSELPHRQDFCLLRWVD"
     gene            complement(7878..>8267)
                     /locus_tag="PLA17_07695"
     CDS             complement(7878..>8267)
                     /locus_tag="PLA17_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85336.1"
                     /translation="AAKTRSVATKAVTVKVAAGSKPAAAEAAATKSVTKDREAARKAT
                     TAKSTPKAVEVVPETAVKKSATQAGEAAPQAGEAIRKAAAKKSTTKAGKAAPKSVKTA
                     PEAAAKKSSKKSAAAKPVAARKRSGSK"
BASE COUNT         1926 a         2210 c         2064 g         2067 t
ORIGIN      
        1 tattgcctct tcccggcttg ccggctctgc atcaagaact gagctctcct tttcggcctt
       61 gtatcccaga tggacgagca gtgatgcgtc gcctccgtca tcaacaatga ggttcgggcc
      121 tttccctccg gggaaggtca gggcctgtgc ggtacaccac cagtactcct ctgccgtctc
      181 acccttccat gcgaataccg ggatgccggc agcggcaatg gcagcagcgg cgtgatcctg
      241 cgtggaaaaa atgttacaac ttgcccatct gacatctgcg ccaagctccc tgagtgtctc
      301 gatgagaacg gctgtctgaa tggtcatatg gagcgagcct gttatccttg cacccctgag
      361 aggtttggaa cccgaatatt tgcgcctgac cgcgagcagc cccggcatct ccttctccgc
      421 aatctctatc tccttacggc cgaatccggc aagtgatata tcctttaccc tgtacttaag
      481 atcgttgtct atgaatgaca taatgataat tttttaggtg tgcaaagtta accaaaaaaa
      541 gggataattg ttcagcggcc cagcagggcg atggtcatct cacggtcgag ggtcagacgg
      601 gctgacagtt catccacact cccgaacttc atctcatcac gcagccggtg acggaatgtt
      661 acagttactt cattcccgta cagatcacca tcgaaatcaa taagatgagc ctcgatggta
      721 cataacccgt caccaccagt gatggtaggc ctgtgtccga tattgagcat ggccatgtgc
      781 ctgtcgggga caccctctac gataacctcc acggcataga ccccggagcg gggtatcagt
      841 ttgtatgcag agagagtggc gatgttggca gtcgggaaac ccatgccacg gccgatcttc
      901 cttccggaaa ccacccggcc cctgatgaaa taatcatagc caagcattga tgcggccttt
      961 tcaatgttcc cctccccgag cgccttcctg atgagcgatg agcttacagc ctccccttca
     1021 accatgtatg cctcctcgcg tgtcaccctg aaagccatcc ttctggcaca ctctatgatt
     1081 gtgttttcgt caccctcccg gcgcctgccg aaatggtggt catacccgat gatcaggtgg
     1141 cagacaccca gatgacggca aagaatggaa ctgataaatt caggggcggt catcctgctg
     1201 agctcagccg tgaaggggat gaccacaaga tagtctatgc ctgtctcccg catgagctcc
     1261 gtccgctctt caaggtctgt caggaagctc agatggcccg gatcgctgga aaggatcatg
     1321 cggggatgag gatcaaaggt gactgccact gcatcagcgc catcttttgc agcctcccct
     1381 ttgacacgcc ggagaagcat cctgtgccca aggtgaacgc cgtcaaagac accgatggta
     1441 ataaccggat tgttaaatga gatgcctgta taaccgtgat agactttcat gacccgattg
     1501 agacatcaaa aataataaaa tcctatcttt atcttgtctg taacatcagg gcaaagagta
     1561 tgacagaaag attttacaag gctgatccgt ggcttgatcc cttcagggag atcatagagg
     1621 cgcggcttgc ctacatcagt taccgtgaga aactgtttac cggtggccgc tccatgaccg
     1681 actatgccaa cggacatcac tggtacggcc tgcaccgtac ccctgaagga tgggtcttca
     1741 gggagcatgc cccgaacgca acgtcgatca ttctcaccgg aacattttca gagtggaagg
     1801 aagatgggcg atatgccctc agccgtacgg cagaaggaga ctgggtgggt gagttcgatg
     1861 caggaatgct ttcgcacggc gacctgtaca agatgattgt caaatggaag ggcggtcagg
     1921 gagaaaggat tccggcctat gccacccgcg ctgttcagga tgaagtgaca aagatcttct
     1981 cggcgcaggt gtggaaccct gaaaacgaat acgtgatgca acacaaggcg cctccgccac
     2041 caccctcact tctcgtttac gaggcacatg tcgggatggc caccgaagaa gaacggaccg
     2101 gcacataccg ggagtttacc agacatgtgc ttccaagggt gaaaagcctt ggctacaaca
     2161 ccgttcagct tatggccatc caggagcatc cctattacgg ctcattcggc taccatgtct
     2221 ccagtctttt tgccccctca tcacgcttcg gcacacccga tgatctcagg gagctgattg
     2281 ataccgccca cagcatgggc ctgagggtaa caatggacct ggttcactcg cacgcagtgg
     2341 caaatatcct tgaagggctc ggcctgcttg acggtgatcc cgggctctac tttcataccg
     2401 gttcacgcag gaagcatgtg gcatgggatt cgctctgcta tgactacggc aagccggaag
     2461 tcaaccattt cctgctgtca aactgcaagt actggcttga ggagttccac ttcgacggat
     2521 tccggtttga cggcataacc agcatgctgt actatgatca cggcctggaa tccgacttcg
     2581 tctcctataa acagtatttt gacggcaacc aggacaagga tgccatcgtc tacatcactc
     2641 ttgccaacaa gctgatacat gaattcaacc aggatgccac caccgtggcc gaggatatga
     2701 gtggcatgcc cggacttgcc gcctccgttg cctccggcgg gatgggtttt gactacaggc
     2761 ttgcaatggg agtgcccgat atctggataa agatggtcaa ggatatccct gatgaggaat
     2821 gggacctcgg gtacctgctc catgaactga cacagcaccg gaccgaggag aaggtgatat
     2881 catactgtga atcacatgac caggcattag taggcgacaa aaccctgatt ttcaggctgg
     2941 cagacaagga gatgtacacc tcaatgagca tcttcacccc cagcctgata gttgaccggg
     3001 ccatggctct ccacaagatg atcaggctct tcaccttcat gacagcaggc ggtggctacc
     3061 tcaccttcat gggtaatgaa ttcggccacc cggaatggat tgatttcccc agagagggaa
     3121 acgggtggag ctataaatac gcgcgcaggc agtggagtct ggcagatgat gaactgctga
     3181 agtacaggtt tctgaatgaa ttcgacaggg caatgattga gaccggtaaa aggcacgggt
     3241 tgccacggct gcagctgaca gagtcactct tagtcaacaa tgctgacaaa atcatcgcat
     3301 tcaggcgcgg tgaccttgtg acggtgatga acttcaatcc gacaatctcc tttaccggat
     3361 acgggattcc cgtcagggga aaattcagga tcatccttga caccgacgat cccatgttcg
     3421 gcgggcaggg caggatagac acaaaccaga cctacttcgc acaacctgac aagggacgtt
     3481 acggggtgac ggctatgcac cacctcaggc tttacctccc cgcaagaaca gctgtggtac
     3541 tcctgcagga gagggtaaag acaatcatgg atctctgaaa tcagtcaacc catctcagca
     3601 ggcagaaatc ctgcctgtga ggcaattcac tgtgcctggc gtagatacgg attccgtaga
     3661 agaaagtgcc ggctatctca ggctggaact caacgctgta tatgacccgt cccttcttcc
     3721 tgttgacata ttcgaactcg tgggacgaga agaaggcatg ctctccgttc ttgagctggc
     3781 gtgtcacaac cagttcaacc ccgacattct ccaccggaat atcctttata tccagaatga
     3841 tctcggtaga gtatttgtat cccgagcgga agacattctt atccaaatcc ttaatgtcaa
     3901 atttaactac ctctatctca tcccatacct ttgagatata gctcttccat ccggccagtt
     3961 cgcgtgccag tgcatagtta tcagcagcca tcctgcggct tctctcccac agcttcttat
     4021 agtacttctc cagataatcg ttgagcatgc ggcgcatggt gaactcggga gccaccttga
     4081 ccatggtgtt cttgatgtag ctgacccatt cacgcggtat gccgctttca tccttcgagt
     4141 agtaggccgg aacgatctca tactcaagca tgttgtagat ggtttcggcg tcaatatcat
     4201 tctgcaattc ctggctggca aaagtccgtt tctttggcag tgcccatccg gcgaaggcac
     4261 gatagccttc cacccaccat ccgtcaagga cactgaagtg aatggttccg ttcattacgg
     4321 ctttttcacc gctggtgcct gatgcttcca ggggccttgt cggagtattc agccatatat
     4381 cagcgccacg tacaaggtaa cgtgccatat ccatatcata gttctccagg aaaataatct
     4441 tcccggcaaa ctgaggcatc tgtgatattt cattgatttt cctgatgagt tcctgtccgc
     4501 cgccgtcatg gggatgagcc ttgccggcat aaatgaactg cacgggtctc tcggggttat
     4561 tgacgattct cgcgagacgg tcgaggtcac ggaacaggag gttggcacgc ttgtatgttg
     4621 cgaatcttct ggcaaatgct attgtaaggg ccttctcatt caggtgagag ctgatgttca
     4681 tcagagtgcg cgggctgaca tgcctctcaa gcatgtctga ctggagacgc ttgcggatat
     4741 tgtcaaacaa gcgcttcttg agcttcatct tgacctcgta aaccttctcg tcttccagct
     4801 tgtgtatctt tgcccattgc ttacggtcac tcagatcaaa atgacgtgtg ccggtgagct
     4861 tctcgtagac ctgttcccac tcgggcgcca cccatgttga atagtgaaca ccgttggtga
     4921 catggcctat gtaaagctcg tcacgaaggt aaccgggcca cagctttgag aagagcccgc
     4981 gtgagacatg cccgtgcagc tgactcactc cgttcatctc ctgggagaag cgggttgcga
     5041 ggaagctcat gttgaacttg cgctcatgat catcctggca gcggcctaag gccatcagct
     5101 gatcccacgt gatattcagc ctggtgtgat aatgtgaaat atattttctc agcaggtctt
     5161 cttcaaaggc atcatggcct gccggcacag gagtatgggt tgtaaacagg gtagatgccc
     5221 tgattatttc cattgactcc gcaaaggtca gatgctcatc ctctatgagg tgacgcatcc
     5281 tctcaaagcc tatgaatgct gaatgccctt cgttgctgtg gtagacatca ggttttattc
     5341 cgatggcaag cagagcccgc attcccccta tgccgaggat cagctcctgc ttcaggcggt
     5401 tctcggagtc gccgccatag agatagtggg tgattgccct gtcctcaggc gtgttatcct
     5461 caaaatcggt gtcgagaaga taaagtttaa ccttgcctac cggagccagc cacacgcggg
     5521 ctttcacaat acgtcctggc caggtaagct gtatggtaag gtgattgccg ttgctctcct
     5581 tcacgggttc aaccggaatc tgagagaaat cctcagcctc ataaatagcc tgctgctcac
     5641 ccctcgggcc cagcttctgc ctgaaataac catacctgta taacagacct atacccgtga
     5701 tattaacgtt ggagtcactt gcctccttca gatagtcacc ggcaagaaca cccaggccgc
     5761 ctgaatagat cttcaggttt ggatgtatgc caaactccat gctgaaataa gctacggagg
     5821 gcaactcctc gttatcaggc ctgccaagat attctgtaaa ctccttgtaa atcctgtcag
     5881 catcatgcag aaacacctca tcgtctgaaa gcactacaag cctcttgtag tcgatggctt
     5941 ccagcaatct cttgggattg tgtccaacct cctcccacag cgtggggtcc atccggcgga
     6001 aaagaaactc agcatcgctg ttccatgacc accagaggtt tgaggagagt tccttgagat
     6061 aagcgatacg tacgggtaac tcagattgca cgtaaatatc tttccagaca ggagcctgga
     6121 agggtttcct tacctgtacc tcggtagctt caagtatgcg ggtgtaatcc ctgggctggt
     6181 ctcttctctt ttcgcttttc tcaagtgcat aacggtatgc attgatataa tagctgaaaa
     6241 gagtattcca gttagctatc cgtgatatct cccaggcctt cccgcgtgca gcggcagcct
     6301 cttcaggatt catctgcata aacttgcgga tgaattcttc catactgaca atgacctcat
     6361 tgtcattgtc gtcagtacgg ttcagtacaa gacatccgtt gccgggttcc ttgaaatagg
     6421 tatcgaccca cataccgaat cctgcaaggt ttgttgtcac ggttggaaca tggaacatca
     6481 ggctctcaag aggtgtgtag ccccatggtt cataataaga gggatagagt gtgagatcga
     6541 acccgataag cagatcatag tatttcatat tgaagatccc gtcattgccg ttcaggtatg
     6601 acggaacgaa tatgaccttt accctttcag aggctatgtt gtgtatgcct tcgcgtatca
     6661 gctggttcat caccgggtca tcattcacat aatggagtcc gtgcgtcagg tatttgtcac
     6721 cttcggtaac cattgtgcca ttggcaaggg cagtggcaag atcctgcctg gggcccttgt
     6781 ggtatgcagg tacgagaata aatgcaacac actgtcggcc ggaattactc ttcctgttca
     6841 cctggcccag tgccgagagg aagaggtcaa tgcccttatt cctgtactca tacctcccgc
     6901 tggtggagac caggagggta tcatccggca ggtcacagcc aatcagagcc tttgccactg
     6961 agagtagcct gtcacgtgct gcattgcgtg ccgcatcaaa attttcaggt tcgggaacaa
     7021 aggtgtcctc aaagccgttg ggagtgatca cgtcaggctt ctttccaaga aacgcctcac
     7081 actcacgccc ggtaatctca cttaccgagg taaagacatc tgcttcctgt gcagtgattt
     7141 tttcaagtga ctgtttggag gtgacattga attcgtgagc tgtctgtacc ggattatatt
     7201 cattgagacg actgtagagt tgcctgttgt tgcctgctat gctgcgaccg agaacagtgg
     7261 cgtgagtggt gaaagcggtg gcaacccagg gggcatgctt ctcaaggtag agaacacccg
     7321 tgccggtcat ccattcatga aaatgggcaa tcacccgagg atattcacgg tagaagttgg
     7381 tgaagctctc gatgattttt cccgctgcgt agccgaataa tgcaggctca atgtaatccc
     7441 actggcctga aatgctgtca agcttgtagg tctcccagaa acgggcaaag atttcattct
     7501 gctgcccgaa atagggcgtg aaatctacca gcatcacgcg cggattgccc ctgatgttcc
     7561 agcgaccggt ttttactatc agaccctcct gcgcggctac ggtcttccat gactggaaca
     7621 tcgtgtcatc aggggtaaac tccggattgg acttgtcgtc cctccataca tcaggaccga
     7681 tcagtatata gttatcttca aattcatagg ttacgtttag cgccttggtt gaaatgactg
     7741 tataaattcc tcctaccttg ttgcatactt cccaactagt ttcgaataaa aaatcagctt
     7801 tcatttccat atagttaata ctcaattaat acgcatgagg cgcagatata ttgcacatat
     7861 gaattatacc ccggttatta tttactgccg gatctcttcc tggcagcaac gggttttgcc
     7921 gcggcactct tcttggaact ctttttcgcg gctgcctccg gggcagtttt tacgctcttc
     7981 ggagctgctt tcccggcctt tgtggtactc ttcttagcgg ctgcctttct tatagcttca
     8041 ccggcttggg gggcagcttc cccggcttgc gtggcgctct tttttacggc tgtctccggg
     8101 acaacttcaa cggccttcgg ggtactcttc gccgtggtag ccttcctggc agcttccctg
     8161 tcctttgtga cactcttcgt cgcagctgcc tccgcagcag caggtttact tccggcggca
     8221 acctttactg tcacggcctt tgtggcaacg ctcctggttt ttgcggc
//
LOCUS       DAOPBK010000118         8238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8030_length_8238_cov_5.974459, whole genome shotgun sequence.
ACCESSION   DAOPBK010000118 DAOPBK010000000
VERSION     DAOPBK010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8030_length_8238_cov_5.974459"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLA17_07700"
     CDS             <1..795
                     /locus_tag="PLA17_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPK85337.1"
                     /translation="SCVPADDPAIDVMTFNIRLDTPHDGPNAWPHRAAIVAEFVNDQI
                     PDLLGMQEVLWHQYLYLDSALAGYGSVAAGRDDGLREGEACPVFYRLGRFDKIDRGTF
                     WLSATPDVPGSIGWGAALTRVATWVKLYDKVKQDTVLFFNTHFDHVSDSARVMSSGVL
                     LGMVRKLAGDNNFVITGDFNALPESLAISRMKEGGFAVDSYLISETPPAGQSYTFNGW
                     KDEPREGRIDYVFVRNGMKVLSHEIHRIIEEGVFISDHWPVSARVR"
     gene            complement(843..1244)
                     /locus_tag="PLA17_07705"
     CDS             complement(843..1244)
                     /locus_tag="PLA17_07705"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPK85338.1"
                     /translation="MMAGNYCHIFPTDKTPEVILEAEGTLVIKGRAMILNKTAPSEKL
                     LDCIDRYLHNPARITTVTIALEYLNSFCTQILVTVLRRIDRVRDQGNMLIVKWYYDKE
                     DDDILERGQHISETFNIPIEFIPIEDTSLFR"
     assembly_gap    1369..1468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1507..2643)
                     /locus_tag="PLA17_07710"
     CDS             complement(1507..2643)
                     /locus_tag="PLA17_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK85339.1"
                     /translation="MKKLLFLLRKEFRQIRRNRFMIRAIIAVPILQMLILVPAITFEI
                     KRIDLAVIDNDRSQASRELVSKLTGSSFFVVTATPATVTEAESMLLSGDADIALVIPP
                     DFSRGLAGVTTPRLQVLADAVNASTAQLSWSYITSVIRDFNREVIVSTGMIPANMAAI
                     RTGSLPGISVSPRYWYNPKLNYKYYMLPGVLVILITAIGLMMTGLNLVREKESGTIEQ
                     INVTPIMKWQLIASRIIPFFIIGLFDLALGLGIGWLAFNIPFEGSLLLFFACAAIFLV
                     AVLGLALFFSTMASTQQQFLFIAFFFVMVFILMSGIFTPTESMPLWAQQFNLVNPVAY
                     LMRINRMVMLKGSGFSEIMRDMAMLTILALSYLSLAIRAYRKRA"
     gene            complement(2717..3838)
                     /locus_tag="PLA17_07715"
     CDS             complement(2717..3838)
                     /locus_tag="PLA17_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK85340.1"
                     /translation="MKRLMAFVRKEFLHIFRDFRTLIILFGIPAAQILIFGYAVRTDL
                     KDAGIAVLDNARDGVSKKLIQKIEASEFFYVKRYLASYEEIDAAFRQGEIKAAIIFPG
                     QIAASVAREGSATIGIIADGSEPNTASLVTGYITGIVNDYSSSLASAVTSSLPLIVPE
                     VRMYFNPGLKSHFMFIPGVITLILILICALMTSVTIVREKEYGTMEVLLVSPLKPWQI
                     IIGKVTPYFFLSLVNVIVILLMTWFVFGLPVRGSIPLLIAGCMLYILLGLSFGILVSA
                     STSKTENAVFISLLTLMLPTILLSGFIFPIENMPKVYDYVSMALPPRWFVGIIRAIMI
                     KGAGFGYIWKETAILAGMTLLIMVISARRFKTRLGEEGR"
     gene            complement(3835..4578)
                     /locus_tag="PLA17_07720"
     CDS             complement(3835..4578)
                     /locus_tag="PLA17_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK85341.1"
                     /translation="MERDKVIEVKDLVKRFGTFTANDHLTFDVWRGEIFGFLGANGAG
                     KTTAIRILCGLSRPTSGRVMVAGIDASQKPEEIKKRIGYMSQRFSLYEDLTVSENIEL
                     YGGIYGMSMKEIVPRREALLEKLSFTSYGDMLIRALPLGLKQKLAFSVAVFHDPEIVF
                     LDEPTSGVDPITRRQFWEMIYETASRGITVFVTTHYMDEASYCDRISIMSEGRIVALD
                     SPSALLRQYGTASVEELFVKIARPQKIRE"
     gene            complement(4578..5489)
                     /locus_tag="PLA17_07725"
     CDS             complement(4578..5489)
                     /locus_tag="PLA17_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK85342.1"
                     /translation="MNAVDAKSITQRFGKITAVDDVSFTVSEGELFGFIGPDGAGKTT
                     LFRIAATLIIPTGGRMTLFGLNVVSDFRELRNHIGYMPGRFSLYTDLTVEENLQFYAT
                     IFGTTIKENYHLIEPVYKQIEPFRKRRAGHLSGGMKQKLALSCALIHKPRILILDEPT
                     TGVDAVSRTEFWEMLERLKREGITIIVSTPYMDEALRCDRIALMQQGRLMGIDTPAAI
                     RSSYGKKIYAVSHRDRYRLLLALRAMTDVDSPEPFGEAVHINIREGATVDDVKKSLER
                     AGLEGAEVTEITPGIEDVFLELMKKGS"
     gene            complement(5588..6469)
                     /locus_tag="PLA17_07730"
     CDS             complement(5588..6469)
                     /locus_tag="PLA17_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPK85343.1"
                     /translation="MKRAVIPIVLLLAAAGCHRGESDADAWGTFMADEVIISAETSGR
                     IISMDVTEGMLVAGGAVIAVTDTTMAVLQRGELDAVTAQAQARLAGIAAQDAVIRQQM
                     DNLSVNIERTRRMLADGAATRKQLDDLTGQMEVLRLQAEANATQRRSVAAEQQGITAK
                     RAVLDEQIARSTVCAPCEATVIEKYASAHELTSAGKPLVKLANMNTMKLKVWVSGAQL
                     AEVKTGTTCTVRIDQGKKEFKNYTGTITHISEKAEFTPKIIQTKEERVTLVYAVTIEV
                     QNDGSIKSGMPGEAIFN"
     gene            complement(6471..7760)
                     /locus_tag="PLA17_07735"
     CDS             complement(6471..7760)
                     /locus_tag="PLA17_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK85344.1"
                     /translation="MKRLTFLTILILPVLPLTAQQTVTLWQCYDSAAVISPLSGEREL
                     YAGMTLLRDRNLAAAWLPSLDLGGSFNYHSDVVDVSEMPGSMPVPPEVLPSIPNEQYR
                     ATVDLSQVIWDGGVTRSAREVERVVSELNMQQSEADIYRLREQVNNLFFPILLVRSQA
                     EVTAVLISELEARISEAESGVKNGVIMPVTLDVLNAEMIKAEQAAAELDRRHEALARA
                     LEQLTGMSGLKYAVLELPDHFITGNEKMENPDLQLFDIRSRQLELSKNLLKSQRMPRL
                     FGFMQAGYGNPPGNNFLSDKADVFYSLGAGLKWNIYDWKKNSNEQKTLTIQQQLLEIR
                     KGAAEESLERMLTLKMAEIESLHEAAGRDKELIEIRSRITAAAASQLKNGTITASQYM
                     TELNNEKQAVIAAAARKISIARAETEYLYITGYKTEK"
     gene            complement(7757..>8238)
                     /locus_tag="PLA17_07740"
     CDS             complement(7757..>8238)
                     /locus_tag="PLA17_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85345.1"
                     /translation="SKDQLFMSVFQVLLKKMFERIMTIVTADITFREKIRQFIDHHID
                     FLNKNPKLPIFLLNEVARNPSVAAELKETFPYEEILDLIYEKHAKELKGYGIKKSDLP
                     QLLITIVSMSVFPYAARHMIAFMMPRMRENRRFKAFMDERKEFATDFILNSLKQRKK"
BASE COUNT         1800 a         2130 c         2149 g         2059 t
ORIGIN      
        1 tcatgtgtac cggctgacga tccggcaata gatgtgatga cattcaacat aaggcttgat
       61 acgccgcatg atggtccgaa tgcctggcca caccgtgctg caattgttgc agagtttgtc
      121 aatgaccaga ttcctgatct gcttggcatg caggaggttc tctggcacca gtacctgtat
      181 ctcgattctg cgctggcagg atacggatca gtggctgctg gtcgtgatga tggcctccgg
      241 gaaggtgaag cctgcccggt gttctaccgc ctgggccggt tcgacaagat tgacaggggc
      301 acattctggc tctcagccac ccccgatgtg ccgggatcaa taggatgggg agcggctctg
      361 acacgtgtgg cgacctgggt gaagctctat gacaaagtga aacaggatac agttctgttc
      421 tttaatactc attttgatca tgtaagcgat tcagccaggg tgatgagctc aggtgtgctg
      481 ctgggaatgg tcaggaaact ggcgggggac aataattttg tgatcaccgg tgatttcaat
      541 gcgctgccgg aaagcctggc gatttcacgc atgaaagagg gtggctttgc agtcgattcc
      601 tatcttatct cagaaacacc gcctgcaggt cagtcgtata ccttcaacgg ctggaaggat
      661 gagcctcggg agggaaggat tgactatgtc ttcgtccgca acggaatgaa ggttttatca
      721 catgaaatcc atcgcattat cgaagagggt gtattcattt ccgatcactg gccggtgagt
      781 gcgcgggtgc gctgacaagg ttgttttccc acggaatatc tggcaggggg atgggggatg
      841 ctttacctga aaagagaggt atcctcgatg gggataaact ctatcggaat gttgaatgtc
      901 tctgaaatgt gctgaccccg ttccagtata tcatcatctt ccttgtcata ataccacttg
      961 acaataagca tgtttccctg gtcacgcacc cggtctatcc tcctcagaac cgttaccaga
     1021 atctgggtgc agaagctgtt caggtactca agggctattg taaccgtggt tatccgggcc
     1081 ggattatgaa ggtagcggtc aatgcaatcg agcaattttt cagacggggc cgttttgttg
     1141 aggatcatgg cccttccttt gataacgagc gtaccttcag cctccagaat gacttccgga
     1201 gtcttgtctg taggaaatat atggcaataa ttgccagcca tcattcagag ccttatctgc
     1261 ctttacaagt gcaaagataa gcagattgaa ctgtctgtgc aaaaaacaaa gcaccggaat
     1321 aactggatac acaggcagga aaggagtggt gtcatgggat atgcaacann nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat atgcaacacg gtgaatccga ccaggtcagg
     1501 taacggttat gcccttttgc ggtatgccct tattgccagt gagaggtatg ataatgcaag
     1561 aatggtcagc atcgccatgt cacgcatgat ttcactgaat cctgatccct tcagcattac
     1621 catgcggttt atgcgcatca ggtaagccac cggattaacc aggttgaact gctgtgccca
     1681 tagtggcatg ctctcggtgg gagtaaatat gccgctcatc aggatgaaga ccatcacaaa
     1741 gaagaatgca ataaagagaa actgctgttg cgtcgatgcc atagtggaaa agaaaagcgc
     1801 cagtccaagc actgccacga ggaatatcgc cgcacatgca aagaagagga gaagactccc
     1861 ctcgaagggt atattgaatg ccagccaccc gatgcccagc cccagtgcca ggtcaaagag
     1921 accgatgatg aagaatggga ttatcctgga tgctatcagc tgccatttca ttatcggggt
     1981 tacgttgatc tgctctatgg taccgctctc cttttccctg acgagattaa gtcctgtcat
     2041 catcagcccg atggctgtta tgagaattac cagcacaccg ggcagcatat agtatttgta
     2101 attcagtttc gggttgtacc agtaccgcgg agaaacggag attcccggca atgaacctgt
     2161 cctgattgct gccatgttgg cgggtatcat cccggtgctg acaatcacct cccggttgaa
     2221 atcgcgtatt accgatgtga tatagctcca tgagagctgt gcagtgctgg cgtttacggc
     2281 atcggcaagc acctgcaggc gcggagtggt gactccggca agtcccctgg aaaaatcagg
     2341 cggaatgacc agtgcaatgt cggcgtcgcc ggagagaagc atcgactctg cctctgtgac
     2401 ggttgccggg gtggccgtca caacaaagaa tgatgaccct gtcagtttag agacaagctc
     2461 acgcgatgcc tgtgaacggt cattatcaat gacggcgagg tctatgcgct tgatttcgaa
     2521 tgtgatagcc ggaacaagga tcagcatctg caatattggt acggctatga ttgccctgat
     2581 cataaaccga ttccggcgta tctggcggaa ctccttgcgc aggaggaaga gaagcttttt
     2641 catcttttgc cctccctgag tttattgctg cctatccatg tgccaatctc tccgggtctg
     2701 gtatggaacc ttatcctcat ctcccttcct ccccgagtct tgttttgaac ctgcgtgcac
     2761 ttatgaccat tatcagcagc gtcatgccgg cgaggatggc cgtctccttc cagatgtatc
     2821 cgaatcctgc ccccttgatc attatcgccc ggatgatccc gacaaaccat cttggcggca
     2881 gcgccatact tacataatcg tacacttttg gcatgttttc aatcgggaaa atgaatccgg
     2941 aaagaagtat tgtcgggagc atcagggtca gtaatgagat gaaaactgcg ttttccgtct
     3001 tgctggtaga ggctgacacc aggatcccga aggagagtcc cagcaggata tagagcatgc
     3061 acccggctat cagcagaggt atgcttccgc ggaccggaag cccgaagacg aaccatgtca
     3121 tcagcagtat cactatcaca ttcaccagcg agaggaagaa gtagggagtc actttcccga
     3181 tgattatctg ccatggtttc aacggcgaca ccagcagcac ctccatggtg ccgtactcct
     3241 tctcgcggac gatggtgaca gaggtcatca atgcgcatat aagtatgaga atgagtgtta
     3301 tgacgcccgg gatgaacatg aagtggctct ttaggccggg attgaagtac atacgcacct
     3361 cgggaactat cagcggcagt gaggaggtaa cggcagaagc aagcgatgat gaatagtcgt
     3421 tcacgatgcc ggtaatgtat cccgtcacca ggctggctgt attcggctct gatccgtcgg
     3481 cgatgatacc tatggtggcg ctgccctcac gggcgacgga ggcggcaatc tgtccgggga
     3541 agatgatcgc agccttgatc tcaccctgcc tgaaggctgc atcaatctcc tcataggagg
     3601 cgaggtaccg cttcacatag aaaaattccg atgcctcaat cttctgaatc agcttctttg
     3661 ataccccgtc acgggcattg tcaagcaccg cgatgccggc atccttaaga tcagtgcgga
     3721 cggcatagcc gaagatgagt atctgtgccg ccgggatgcc gaacaggatt atcagggtcc
     3781 ggaaatcgcg gaagatgtgg aggaactcct ttctgacaaa tgccattaac cttttcattc
     3841 cctgatcttt tgaggtctgg ctatcttcac gaacagctct tcaacagatg ccgtgccgta
     3901 ctggcggagc agggcagagg gggagtcgag ggctactatc ctgccctcac tcatgatgct
     3961 gatacggtca caatagctgg cttcatccat gtagtgcgtt gtgacgaaaa cagtgattcc
     4021 acgcgaggcg gtttcataga tcatctccca gaactggcgc cgcgtaatgg gatcgacacc
     4081 gctggtcggc tcatcgagga agactatctc cggatcatga aataccgcca cggagaatgc
     4141 cagcttctgt ttcagcccga ggggcagcgc cctgatcagc atgtcaccgt atgaggtgaa
     4201 gctgagcttt tcaagaagcg cctcacggcg gggaacgatc tccttcatgc tcataccgta
     4261 tatgcctccg tagagctcga tgttctcact cacggtgagg tcttcataga gggagaagcg
     4321 ctgcgacatg tatcctatcc gtttcttgat ctcctcgggc ttctgcgagg cgtcgatgcc
     4381 ggcaaccatg acccttcctg aggtgggtcg tgacaggccg cagaggattc ttatggcagt
     4441 ggtcttgccc gcgccattgg ctcccaggaa cccgaatatc tccccgcgcc agacgtcaaa
     4501 ggtgagatgg tcattggccg tgaatgtgcc aaaacgctta accagatcct ttacctctat
     4561 gactttatcc ctctccatca gcttcccttt ttcatcagtt cgagaaacac atcctcaatg
     4621 ccgggggtga tctccgttac ttcagcgccc tccaggccgg cacgttccag cgacttcttc
     4681 acatcatcca cggttgcacc ttcccgtata tttatatgga ctgcttcgcc gaaaggctcc
     4741 gggctgtcaa catcagtcat tgcccggagt gccaggagga gcctgtacct gtcgcgatgg
     4801 ctcacggcat agattttctt accgtatgat gagcgtatgg ctgccggagt gtctatgccc
     4861 atcagcctcc cctgctgcat aagggcgatg cggtcacagc ggagtgcctc gtccatgtat
     4921 ggtgtggaaa caatgatggt tatcccctcc cttttcagtc gctccagcat ctcccagaat
     4981 tcagtgcgcg agacggcatc caccccggtg gtgggctcgt cgagaatcag tatcctcggc
     5041 ttgtgtatca gtgcgcagga gagggcaagc ttttgtttca ttcctcccga gagatgaccg
     5101 gcgcggcgtt tcctgaaggg ttctatctgc ttgtataccg gttctataag gtggtagttc
     5161 tcctttatgg ttgtcccgaa gatggttgca tagaactgca ggttctcctc cacggtcaga
     5221 tccgtgtaga ggctgaagcg gccgggcatg taaccgatat gattacgcag ctcccggaag
     5281 tcgctcacca cattgagccc gaagagggtc attcgtcccc cggtgggtat gatcagggtt
     5341 gccgcgatgc ggaagagggt tgttttaccg gctccgtcag gaccaataaa gccgaagagc
     5401 tcaccctcac tgacggtgaa cgagacatcg tcaacagcag tgattttgcc aaacctttgg
     5461 gttatgctct ttgcatctac ggcattcatc tcatttatac tatactttgg tgcgctggtt
     5521 tacagctggt tttcgttatt gccgccgggt catcagtcac tgtaagattg catcgcggag
     5581 ccagatatca gttgaatatt gcctctccgg gcatgcctga cttgattgag ccgtcgtttt
     5641 gcacctcaat ggtaaccgcg tagaccagtg tcacccgctc ttcttttgtc tgaatgattt
     5701 taggagtgaa ctcagccttt tctgatatat gggtaattgt gcccgtgtag tttttgaatt
     5761 cctttttacc ctggtcgatg cgtacggtgc atgttgttcc tgttttcacc tctgccagtt
     5821 gtgcaccgct gacccacacc ttcagcttca tggtattcat gtttgccagc ttcaccagtg
     5881 gtttgcctgc tgatgtcagt tcgtgtgccg aggcatattt ctcaataacg gttgcctcgc
     5941 agggagcgca gacggtgctg cgggctatct gttcatctag tacggcccgt ttggctgtta
     6001 tgccctgctg ttctgccgcc actgagcgtc gctgtgtggc gttggcctca gcctgcaggc
     6061 ggagcacctc catctgtccg gtgaggtcat cgagctgttt gcgggtggcg gcgccgtcag
     6121 cgagcatgcg gcgcgtacgc tcaatgttga ctgacaggtt atccatctgc tggcgtatga
     6181 ccgcgtcctg cgccgctatg cccgccaggc gcgcctgcgc ctgcgccgtc accgcatcaa
     6241 gctcaccacg ctgcaacacc gccatcgtcg tgtcggtgac ggcgatgacg gcgccacccg
     6301 ccaccagcat accctccgtc acatccatgc tgatgatcct gcctgaggtc tcggcagaga
     6361 tgatcacctc gtcagccatg aaggtgcccc aggcatcagc gtcgctctca ccgcggtggc
     6421 atccggcggc ggccaggagc agaacaattg gtattaccgc tcttttcata ttatttctct
     6481 gttttgtatc ctgttatata gagatattcc gtttcggccc tggcaatgct tatcttcctg
     6541 gccgctgccg caatgacggc ctgcttctca ttgttcagct ccgtcatgta ctgcgaggca
     6601 gtgatagttc cgttcttcag ctgcgatgcc gcagcggcag tgatgcggct gcgaatctct
     6661 atcagctcct tgtcgcgacc agcagcctca tgcagagact caatctctgc catcttaagg
     6721 gttagcatac gctccagcga ctcttccgct gcacctttcc tgatttccag aagctgctgc
     6781 tgaatcgtca gggtcttctg ttcattgctg ttctttttcc agtcgtatat gttccatttc
     6841 agtccggcac ccagcgagta aaagacgtct gccttatcgg aaagaaagtt gtttccgggt
     6901 ggattgccat agcctgcctg catgaaacca aagagcctcg gcatcctctg actcttcagg
     6961 aggtttttgg ataattcaag ctgccggctg cggatatcaa agagctgcag gtcaggattc
     7021 tccatctttt cattgccggt tatgaagtga tcgggcagtt ccagcacggc atatttcagc
     7081 ccactcatcc cggtgagttg ctccagggca cgagccagcg cttcatgcct tcgatcgagc
     7141 tcagcagcag cctgctcagc ctttatcatc tccgcattaa gaacgtcaag ggtaaccggc
     7201 atgatcaccc cgttcttcac tcccgattcg gcttcactga tacgggcttc cagctcagag
     7261 atcagcaccg cggtcacttc agcctggctt cgtacgagga ggatcggaaa gaagaggttg
     7321 ttaacctgtt cacgaagacg atatatatcg gcttcgcttt gctgcatatt tagctcgctg
     7381 accacccttt ccacctcgcg tgcgctgcgg gtcaccccgc cgtcccagat aacctggctc
     7441 aggtcaaccg tggccctgta ctgctcattc ggtattgagg ggagcacctc cggaggtacc
     7501 ggcatggagc caggcatctc ggaaacatca acaacatccg aatgatagtt gaatgagccc
     7561 cccaggtcaa gggagggaag ccatgcggca gcgaggttac ggtcacgcag cagtgtcatg
     7621 cccgcgtaga gttctctctc tcccgagaga ggcgatatga cagcggcaga gtcgtagcac
     7681 tgccacaggg ttactgtctg ctgtgccgtg agaggcaata ccggcaggat cagtatcgtc
     7741 agaaaggtca gtctcttcat tttttccttt gtttcaggga gttgaggatg aagtcggtgg
     7801 caaattcctt ccgttcatcc atgaatgctt taaaccttct gttttcacgc atccggggca
     7861 tcatgaaggc tatcatgtgg cgagccgcat aggggaatac cgacattgaa acaatggtga
     7921 tcagcaactg cggcaaatca ctttttttta tgccgtaacc cttgagttcc tttgcatgct
     7981 tttcgtatat caggtcaagt atctcttcgt acgggaatgt ctccttcagt tccgcagcaa
     8041 cggaaggatt ccttgcgact tcgttaagaa ggaaaatagg cagtttggga tttttgttca
     8101 ggaagtcaat gtgatgatca ataaactgtc ttatcttttc cctgaaagtg atgtcggcag
     8161 taactattgt catgattctt tcaaacatct tcttcagaag cacctggaat actgacataa
     8221 agagctgatc cttgctgc
//
LOCUS       DAOPBK010000119         8128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8136_length_8128_cov_5.070730, whole genome shotgun sequence.
ACCESSION   DAOPBK010000119 DAOPBK010000000
VERSION     DAOPBK010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8136_length_8128_cov_5.070730"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLA17_07745"
     CDS             <1..460
                     /locus_tag="PLA17_07745"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPK85346.1"
                     /translation="KKTAAALRRAGADIIIGSHPHVVQPVAAEKDSAGIRNPVVWSMG
                     NFVSNQRTRRRDGGIMIRLDITAKGDTAFISDAGYVLTWVYTPVENGKKKFYILPCAE
                     FEKKPELFQSSGHYDSMMLYVKDARRLLDNHGSGFREMTLTDGKWIGVTR"
     gene            507..1217
                     /locus_tag="PLA17_07750"
     CDS             507..1217
                     /locus_tag="PLA17_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPK85347.1"
                     /translation="MDNSTILLAGTAVTIGFIHTLLGPDHYLPFIVMGEARKWTIRKT
                     MFITFLCGLGHVLSSVIVGFIGIAAGISLSKLEFLEGFRGNIAAWMLIAFGLVYTLIS
                     LRNVYRRKQHVHTHTHADGITHEHRHDHYSDHSHIHLNERKNLTPWILFLIFVLGPCE
                     PLIPILMYPAAENNISGVIIVSLLFSAVTIATMMAVVLAFRLGLSTINLKPLERWVNV
                     IAGATIAVSGLAIRFLGL"
     gene            1258..1905
                     /locus_tag="PLA17_07755"
     CDS             1258..1905
                     /locus_tag="PLA17_07755"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HPK85348.1"
                     /translation="MNFDYNTQRKRMALPEYGRNVLKMIEHAKTIKDPEERNRAAKTI
                     IQIMGNLNPNLREVTDFKHKLWDHIMIIADFDLDVDSPYPAPDRKKLNSKPNKVPYHN
                     GEIKYSHYGCIIPAMIEAAAKMKDGEEKDYLTSLILNQMKKDYVTWNKSTVADEVIIR
                     DLIEISGNRLKVPENYKMPDVRDLIAQPKGKTQGKHQGRSQGRSKQNKKKHKNGY"
     gene            1926..2309
                     /locus_tag="PLA17_07760"
     CDS             1926..2309
                     /locus_tag="PLA17_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HPK85349.1"
                     /translation="MSLINVITYDAPLPGGHYSQAIVSGNMIFVSGQLPVIPLTGDKL
                     TGTISEQTLQALKNVLSIVKAAGGDITTIARTTIYTTDVKYWEEINRVYADFFGQMLP
                     ARSIITVAGLHHGCLIEIEAVAFTL"
     gene            2380..2970
                     /locus_tag="PLA17_07765"
     CDS             2380..2970
                     /locus_tag="PLA17_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPK85350.1"
                     /translation="MVKKKSQEERHDEPTPKKKVTDNQINTENDASRQHVPEDEPEAA
                     VKSQAEKELLGKLTEMQDRYLRLSAEFDNYRKRTLKEKIELSQSGGESVIKSLLPVID
                     DFDRAMNSMRSTSDYNAVKEGLELIYSKINDFLKQNGVKEIEVMNEAFNGDFHEAVTT
                     VAVEDDKLKGLIIDVTQKGYTLNDKVIRYPKVVVGE"
     gene            2993..4147
                     /gene="dnaJ"
                     /locus_tag="PLA17_07770"
     CDS             2993..4147
                     /gene="dnaJ"
                     /locus_tag="PLA17_07770"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPK85351.1"
                     /translation="MSKRDYYEVLGVSKGASKDEIKKAYRKQALKYHPDKNPGNKEAE
                     EKFKEAAEAYEVLSNDEKRARYDQFGHAGMNGGGFGGGFGGGMTMEDIFSSFGDIFGD
                     AFGGAFGSRRGGGSRSRSVNKGSNLRLKVKLTLSEFAHGTEKRVKVNKYVACNACHGS
                     GAASDSDVTTCTACKGTGHTTRVMNTILGQMQTTAPCNVCGGEGKVITKKCHKCYGEG
                     ITQAEEVIPISIPAGLAPGMQLSVTGKGNAARRGGINGDLIVVIDEEPHDELIREGND
                     LIYNLFISIPDAILGANVEIPTIDGKVRIKIEPGTQGGKILRLRGKGLPDVNGYGKGD
                     LLVNVNVWIPKNLSREEQRAFEQFKNSASFTPKPDADDKGFFDRMKSYFS"
     assembly_gap    4206..4305
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4370..5296
                     /locus_tag="PLA17_07775"
     CDS             4370..5296
                     /locus_tag="PLA17_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK85352.1"
                     /translation="MIIFEAKEVTKRFAAQIALDRVSIRVPQQSIYGLLGPNGAGKTT
                     LIRIINQITAPDSGVILLNGRPISPDDVNNIGYLPEERGLYKKMKVGEQALYLAQLKG
                     LTHAEAMRRLKYWFKKLDIIDWWDKKVEELSKGMQQKVQFVTTVLHEPKLIILDEPFT
                     GFDPINTRQIKDEILFLRERGATIILSTHDMGSVEELCDNITLINKARTILEGSVEKI
                     RNEWAGNEFEVVFSGEVNPLSNANLTVLSSVFEDGKSRVLIKASEPVNNNAILREMTA
                     AGQVLSFNPALLSMNEIFIRVVEAKNKEAVKN"
     gene            5300..6658
                     /locus_tag="PLA17_07780"
     CDS             5300..6658
                     /locus_tag="PLA17_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK85353.1"
                     /translation="MNKTAIIIRREYLTRVRKKSFLIMTFLTPFLMALLFIIPALVMT
                     NEDKEFKKIAVIEDGSDLFVNVIPDTRDTEFEYLQGVKLNDIKNNFSELGYYGVLYIS
                     PDLVANPDAIQLLSRKQPPIGLLDHISGSLEKEIERQKLLAYNIEDLDEILRAINSSV
                     RIQTIKIDETGSARETSTDIAMALAYIGGFLMYMLVFMFGAMVMRGVIEEKTSRIVEV
                     IVSSVKPVQLMLGKIIGVALVGLTQFALWVVLTLALVLIVKANVLPEGTMEQLQQLPQ
                     SFAEADQAMAAGSETTTMTAEEFTMFQKIFAGALNMPWGLIIFSFVFYFLTGYLLYAS
                     LFAAIGSAVDNETETQQFMLPVTIPIILALVVAMGTMQNPESSLSFWFSIIPLTSPIV
                     MIARIPFDVPPWEIALSMGLMILTIVGTIWMAAKIYRTGILMYGKKTSYKEIWKWLTY
                     KG"
     gene            6671..7849
                     /locus_tag="PLA17_07785"
     CDS             6671..7849
                     /locus_tag="PLA17_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK85354.1"
                     /translation="MSRILVIDDEKSIRNTLKDVLEYEKYEIDLAEDGTSGIELFGQQ
                     QYDAVLCDIKMAKMDGIEVLQKLLEISPDMPVIMISGHGNIDTAVDSIKKGAFDFLEK
                     PLDLNRLLITIRNALERGSLITQTKVLKKQVSKRYEIVGNSPAILKVKEMIDRVAPTE
                     ARVLITGANGTGKELVAHQVHEKSNRARGPFVEVNCAAIPSELIESELFGHEKGSFTS
                     AIKQRIGKFEQASGGTIFLDEVGDMSLAAQAKVLRALQENKITRVGSDKDIHVDVRVV
                     AATNKNIREEIDHNRFREDLYHRLSVILIHVPTLNERQEDIPLLADHFNTLICGEYGM
                     GKKKIKPDAIAELQKIIWTGNIREFRNVLERLIILCPEEITGRDVIAFAHPVSGFGDI
                     "
     gene            7846..>8128
                     /locus_tag="PLA17_07790"
     CDS             7846..>8128
                     /locus_tag="PLA17_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85355.1"
                     /translation="MRRRAAISVLTAVLLFLTACGRKATDSIPLSFNDGWLFIRQDSA
                     NADKILPGIIPNHKWEEVTLPHSAVIEPLVVTGSQWTGICWYKKVYKPDR"
BASE COUNT         2286 a         1902 c         2066 g         1774 t
ORIGIN      
        1 gaagaagacg gcagcagcgc tgcggagggc aggcgctgat atcatcatcg gctctcaccc
       61 ccacgtggtg caaccggtgg cagctgaaaa agactctgcc ggaatcagaa accctgtagt
      121 atggtccatg ggcaacttcg tctccaacca gaggacaagg agaagggacg gcgggataat
      181 gatcaggctg gatataactg cgaaaggtga cactgcattt atttctgacg ccggatatgt
      241 tcttacctgg gtctacactc ccgtggaaaa tggcaaaaag aagttctaca tcctgccctg
      301 cgctgaattt gaaaagaaac cggaattgtt tcagtcttcc gggcattatg attcaatgat
      361 gctctacgtt aaagatgcca gacggctgct tgacaaccat ggatcagggt tcagggagat
      421 gaccctgacc gatggaaagt ggataggggt taccagataa ccggaccggc aatatcatta
      481 tctttgcaca gaattagaat acggtaatgg ataattcgac tatactactg gcaggcactg
      541 ccgtaaccat cggtttcatt catacactgt tggggcccga ccactatctg ccgttcattg
      601 ttatgggcga ggcgcgtaaa tggaccatca ggaaaacgat gttcattacc tttctctgcg
      661 gcctcggaca tgtcctgagt tcggtaatcg tgggattcat aggtattgct gcaggtatat
      721 ccctttcaaa actggaattc ctcgaaggat tcagggggaa tatagcagcc tggatgctaa
      781 tagccttcgg actggtgtac acccttatca gccttcgcaa tgtctaccgc agaaaacagc
      841 atgttcacac ccatacccac gctgatggta tcacgcatga gcaccggcat gaccactaca
      901 gtgaccactc gcatatacac ctgaatgaga ggaagaacct cactccgtgg atacttttcc
      961 ttatatttgt actgggcccg tgcgagccgc tgatacccat tctcatgtac cctgcagcag
     1021 agaacaacat cagcggtgtg ataattgtat cactgctttt ctccgcggtc acaatcgcca
     1081 ccatgatggc agttgtcctg gctttcaggc tgggtctcag caccatcaac ctgaaacccc
     1141 tcgagcgttg ggttaatgtg attgccggag ccaccattgc agtttcaggc ctcgccatca
     1201 ggtttcttgg cctttgaaaa tgattttatt agtagtttaa acaattatat ctaacagatg
     1261 aatttcgatt acaatacaca gagaaaaaga atggcgctgc ctgaatacgg gaggaatgtc
     1321 ctgaagatga ttgaacatgc caagacaatc aaagatccgg aggagaggaa ccgggcagcc
     1381 aaaaccatta tacagataat gggcaatctg aatcccaacc tcagggaggt gactgatttc
     1441 aagcacaaac tctgggatca catcatgatc attgctgatt ttgaccttga cgttgattcg
     1501 ccttatcctg cccctgaccg gaagaagctt aactccaaac caaataaagt cccctatcat
     1561 aacggggaaa taaaatattc tcattacggg tgcatcatcc ctgccatgat tgaggcagca
     1621 gcaaagatga aggatggtga ggaaaaggat tatctcacgt cgctcatcct caaccagatg
     1681 aagaaagatt atgttacctg gaacaagtca acggttgctg acgaagtcat catcagggac
     1741 ctgattgaaa tatcaggcaa caggttaaag gtgcccgaga actacaaaat gcccgatgtc
     1801 agggatctga ttgcccagcc taaaggcaag acacagggca agcatcaggg cagatcgcag
     1861 ggaaggtcta agcagaacaa gaagaagcac aagaacggct actaattaat catatctttc
     1921 agaccatgag cctcatcaat gttatcacct atgacgctcc attgccgggc gggcactatt
     1981 cacaggcgat tgtctccgga aacatgatct ttgtttcagg ccagttgccc gtcattcccc
     2041 tgacaggaga caagctgacc ggcaccatca gcgaacagac cctgcaggcg cttaaaaatg
     2101 tgctcagtat cgtgaaggca gccggtggtg acattacaac cattgcccgc acaaccatct
     2161 atactaccga tgtgaaatac tgggaagaga taaacagagt gtacgccgat ttctttggtc
     2221 agatgctccc ggccagatcc ataatcaccg tagcaggtct gcaccacgga tgcctgatag
     2281 agattgaggc cgtggcattc acactttaaa ttgccacttt gtcagtttta tcattgtggc
     2341 cggcattttg tttatggccg ttagtaaatt gtttttatca tggtaaaaaa gaagagtcag
     2401 gaagagaggc atgatgaacc cacgcccaaa aagaaagtaa cagataatca aataaataca
     2461 gaaaatgacg cctccaggca gcatgtgcct gaagatgaac ctgaagccgc tgtgaagagt
     2521 caggcagaga aagaactact cggaaaactg acagaaatgc aggacaggta cttacggcta
     2581 tcagctgaat ttgacaatta caggaagaga accctgaagg aaaagatcga gctgtcacag
     2641 tcaggcggtg aatcggtaat caaaagcctt cttcccgtca ttgatgattt tgacagggcc
     2701 atgaactcta tgcgttccac cagtgactat aatgctgtga aagagggact tgagctgata
     2761 tacagcaaga taaatgattt cctgaaacag aacggagtga aggagataga ggtcatgaac
     2821 gaggcgttca acggtgattt ccacgaggca gtcacaaccg ttgcagtgga agatgataag
     2881 ctgaaagggc tgatcattga cgttacccag aagggatata cacttaatga caaagtgatc
     2941 cgctacccga aggtggtagt aggtgaataa ctgaatgctt ctgttctgaa acatgtcgaa
     3001 gagagactat tatgaagtac tgggcgtaag caagggcgct tcgaaggatg agatcaagaa
     3061 ggcctacagg aagcaggccc tcaagtatca tcctgacaag aatcccggca acaaggaggc
     3121 cgaggagaag ttcaaggagg ctgccgaagc ttatgaggtt ctcagcaatg atgagaaacg
     3181 ggcgaggtat gaccagttcg ggcatgccgg catgaacgga ggcgggttcg ggggcggatt
     3241 cggaggcggg atgaccatgg aagatatctt ctcgtcattc ggcgacatct tcggtgacgc
     3301 tttcggcggg gctttcggat cacgtcgcgg cggaggcagc cgctcacgca gcgtcaacaa
     3361 gggctccaac ctgaggctaa aggtgaaact caccctttca gaatttgccc atggcaccga
     3421 gaagagggtt aaggtgaaca aatatgtcgc ctgcaatgcc tgccatggca gcggggctgc
     3481 ttctgacagt gatgtaacta cctgtaccgc atgcaagggc accggccaca ccaccagggt
     3541 tatgaacacc atcctggggc agatgcagac cacagcaccc tgtaacgtct gcggcggaga
     3601 gggcaaggtt attaccaaaa aatgccataa gtgctatggc gaagggatta cgcaggcaga
     3661 ggaggtgatc cccatttcaa tcccggcagg gcttgccccg ggcatgcagc tttccgtgac
     3721 cggcaagggc aacgcggcac gccgcggcgg catcaatggc gacctgattg tagtcataga
     3781 cgaggaaccc catgatgagc tcatacgtga aggcaatgac ctgatataca acctgttcat
     3841 cagtataccc gatgcaattc tcggcgccaa tgtcgagatt ccgaccattg acggcaaggt
     3901 aaggatcaag atagaaccgg gaacccaggg aggaaagata ctacgcctca ggggcaaggg
     3961 actgcctgat gtgaacgggt atggcaaggg tgacctgctg gtgaatgtga atgtctggat
     4021 accgaagaac ctctcaaggg aggaacagag agccttcgaa cagtttaaaa actcagcatc
     4081 gtttaccccg aagcctgacg ccgatgacaa gggcttcttt gaccggatga agagttattt
     4141 ctcctgacag ttgcaaaaga gttgtttcac ctgacaacca caaaaagagt tacttctcct
     4201 gacaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtatc agcctttttt
     4321 atatctttga aattctctga ctgaatgctt tattaattgt gtaaaaacaa tgataatatt
     4381 tgaagccaaa gaggtgacga aacgctttgc tgcccagatt gcgcttgaca gggtcagcat
     4441 aagggttccc caacagagca tctacggcct tctggggccg aacggagcag gcaagacaac
     4501 acttatccga atcattaacc agatcacggc gcctgacagc ggagtcattt tactgaacgg
     4561 acgcccgata agccccgatg atgtgaataa catcggctac ctccctgagg agcgcggact
     4621 gtacaagaaa atgaaggtgg gtgaacaggc actctacctc gcacagctta aaggactcac
     4681 tcatgcggaa gccatgaggc gcctgaaata ctggttcaaa aagcttgata tcatcgactg
     4741 gtgggacaag aaggtggagg aactgtcgaa gggcatgcag cagaaggtgc agttcgtaac
     4801 cactgtcctt cacgaaccga aactgattat cctggacgaa ccattcacgg gatttgaccc
     4861 gatcaatacc aggcagataa aggatgagat cctgttcctc cgcgaacgcg gtgccacaat
     4921 cattttatca acacatgaca tgggctctgt tgaggaactg tgtgacaata taacccttat
     4981 caacaaggcg cggacaatcc tcgagggcag tgttgaaaag atccgcaatg agtgggcagg
     5041 caacgagttc gaggttgttt tctccggtga ggtgaacccc ttgtcaaacg ctaaccttac
     5101 agtgctctct tcggtctttg aagacggcaa gagcagagtg ctcatcaagg catcagagcc
     5161 tgtcaataat aacgccatcc tcagggagat gaccgctgcc gggcaggtac tctcgttcaa
     5221 cccggccctc ctgtcgatga atgagatatt cataagagtg gtggaagcca agaacaagga
     5281 agctgtaaaa aactgagcca tgaacaagac agctattatc atacgcaggg aatatcttac
     5341 cagggtacgg aaaaagtcgt tcctcattat gacctttctt accccgttcc tcatggccct
     5401 tcttttcatc atcccggcgc tggtgatgac caatgaggac aaggagttca agaagatagc
     5461 agtgattgaa gacggatctg acctcttcgt caatgtgatc cctgacacca gggatactga
     5521 attcgagtac ctgcaagggg tgaaactgaa tgacatcaag aacaactttt cagagttggg
     5581 gtactatggt gtactctata tctcccctga cctggttgcc aatccggatg ccatccagct
     5641 tttatcgcga aagcaaccgc ccatagggct gcttgaccac atctccggat cacttgaaaa
     5701 ggagatcgaa agacagaagt tgcttgccta caacattgag gacctcgatg agattctacg
     5761 cgcaatcaac tccagcgtca ggatacagac aataaagata gatgagacgg gcagtgcccg
     5821 cgaaaccagt acagacattg cgatggcgct tgcttacatc ggcggattcc tgatgtacat
     5881 gcttgtattc atgttcgggg ctatggtcat gcgtggtgtg atcgaggaga agaccagccg
     5941 catagtggaa gtgatagtat catctgtcaa gcctgtacag ctcatgcttg gaaagatcat
     6001 tggtgttgcc ctcgtgggac tgacacagtt cgcactctgg gtagtgctga cactcgcgct
     6061 ggtcctgatt gttaaagcca acgtcctccc ggagggaacc atggaacagt tacagcaact
     6121 gccccagagc tttgccgagg ctgaccaggc aatggctgcc ggatcagaaa ccactaccat
     6181 gacagcagag gagttcacca tgtttcagaa gatattcgcc ggcgctctca acatgccatg
     6241 gggactgatt atcttcagtt tcgtcttcta tttccttacc ggttacctgc tttatgcctc
     6301 gctctttgcc gccatcggtt cggctgttga caatgagaca gagacgcagc aattcatgtt
     6361 gccggttacc atcccgataa tactggccct ggtggttgcc atgggaacca tgcagaaccc
     6421 tgagagctca ctctctttct ggttctcaat cataccgcta acctccccca ttgtaatgat
     6481 agcccggata ccctttgacg taccgccatg ggagatagca ctctcaatgg ggctgatgat
     6541 tctgacaata gtgggtacaa tatggatggc ggcaaagatt taccgtaccg ggatacttat
     6601 gtacggcaag aaaacatcat acaaggaaat atggaagtgg ctgacataca aaggataagg
     6661 attagcaaga atgtcaagga tactggtcat agatgatgaa aaatccatca ggaacaccct
     6721 gaaggatgta cttgaatacg agaaatacga gattgatctg gctgaggatg gcacatcagg
     6781 gatcgagctt ttcgggcaac agcagtatga cgccgttctc tgtgacatca aaatggcaaa
     6841 gatggacggg attgaggtgc tgcagaaact gctggagatt tcacccgata tgccagtgat
     6901 catgatctca ggtcatggaa acatagatac ggctgttgac tccattaaga aaggggcctt
     6961 tgacttcctt gaaaagcccc ttgatctcaa ccgcctgctc ataaccattc gcaatgcgct
     7021 tgagagaggc tcactgatca cccagaccaa ggtactgaag aaacaggtca gcaaaaggta
     7081 cgagattgtg ggcaactcac ctgcaatatt gaaggtcaag gagatgattg acagggttgc
     7141 tcccaccgaa gcccgtgtgc ttatcaccgg cgccaacggc accgggaagg aactggtggc
     7201 gcaccaggta catgaaaaga gcaaccgtgc tcgcggcccc tttgtggaag tcaactgcgc
     7261 ggctataccc tccgagctga ttgaaagcga actcttcggt cacgaaaagg gatcattcac
     7321 ctcggcaatc aaacagcgta tcggcaagtt tgaacaggct tcgggaggca ctatctttct
     7381 cgacgaggtg ggagacatga gcctggcagc acaggcaaag gttctcaggg cactgcagga
     7441 aaacaagata acccgggtgg gatctgacaa ggatatccac gttgacgtca gggtagtagc
     7501 cgcaaccaac aaaaatatca gggaggagat tgaccacaac agattcaggg aagacctcta
     7561 ccaccgcctc agcgtgatac tgattcatgt gcctaccctc aatgaaaggc aggaagacat
     7621 acccctcctt gcagatcatt tcaacactct catctgcggt gaatacggta tgggaaaaaa
     7681 gaagataaaa cctgatgcca tagctgagct gcagaaaatc atctggaccg gcaacataag
     7741 agaattcagg aatgtgctcg aaagacttat catcctgtgc ccggaggaga taaccggcag
     7801 ggatgtgatt gcatttgcac acccggtatc aggattcgga gatatatgag aagaagagcc
     7861 gccatatcag tgctgacggc tgtgctgttg ttcctcactg cctgcggcag gaaggcaact
     7921 gacagcatac ctctttcgtt caatgacggc tggctcttta tcaggcagga ctcagccaat
     7981 gcagacaaga tacttccggg aataattccc aaccataaat gggaagaggt gacactgccg
     8041 cattcagcag taattgaacc gctggtagtt acaggaagtc agtggaccgg tatatgctgg
     8101 tataagaagg tatataagcc tgacagga
//
LOCUS       DAOPBK010000120         8082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8198_length_8082_cov_4.675969, whole genome shotgun sequence.
ACCESSION   DAOPBK010000120 DAOPBK010000000
VERSION     DAOPBK010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8198_length_8082_cov_4.675969"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..346)
                     /locus_tag="PLA17_07795"
     CDS             complement(95..346)
                     /locus_tag="PLA17_07795"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HPK85356.1"
                     /translation="MAAQHGQLNISLSFKQVVDIVKQLSPSEKQMLSELLWMEQDIDD
                     IVIPEEHKQIVRERIKKYENSPDSYLSWNDIERKMSARE"
     gene            complement(524..611)
                     /locus_tag="PLA17_07800"
     tRNA            complement(524..611)
                     /locus_tag="PLA17_07800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(575..577),aa:Ser,seq:gga)
     gene            complement(730..1761)
                     /locus_tag="PLA17_07805"
     CDS             complement(730..1761)
                     /locus_tag="PLA17_07805"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HPK85357.1"
                     /translation="MMEKNRSILIIYTGGTIGMVNDPVSGTLVPIDFRHISNQVPELR
                     RFGFSLETITFDPVIDSSEVSPDTWVHIAKTIEENYARFDGFVVLHGTDTMSYSASAL
                     SFMLQGLEKPVIFTGSQLPIGVLRTDGKENLLTAIEIAAATGDGLPMVPEVCIFFGSR
                     LFRGNRTIKYSAEHFNAFASPNLPPLAEAGLQIRYNRSIIRHPTVRRPLMVSDNIETA
                     VAVLRLFPGIRQETVHTLLTQAGLKGIILETYGTGNAPMSGWFLEELRSFISGGGIVL
                     NVTQCQAGSVEMGLYKTSAGLISAGVISGRDLTTEAAVTKMMVLLGRGLPEGKVSNLL
                     SMSICGEMS"
     gene            complement(1762..1932)
                     /locus_tag="PLA17_07810"
     CDS             complement(1762..1932)
                     /locus_tag="PLA17_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPK85358.1"
                     /translation="MLETDAPYLAPVPHRGKRNEASFLTYVIDRLSTILSLSPEAIAR
                     RTTENARRLFNL"
     assembly_gap    1940..2039
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2088..3197
                     /gene="dprA"
                     /locus_tag="PLA17_07815"
     CDS             2088..3197
                     /gene="dprA"
                     /locus_tag="PLA17_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPK85359.1"
                     /translation="MSLMPLVYKIALGMIPGVGDITARRLVSYAGGVEAVFRESFRSL
                     TRIPGIGEGLASAIAGHGYLPAAEREAEFVTRNNIRVYFYLDDDYPVRLRQCEDSPVT
                     FYFRGNADLDSPMILSVVGTRHATENGRRLCQKIVSGLAEHFPGLLIVSGLAYGIDIT
                     AHRAALTAGLPTVAVLGHGLKTVYPSVHAGVAKAMLEKGGHVTDFSSPTLPERNNFIR
                     RNRIIAGISDATLIIESGIKGGALITADIAASYNRDVMAVPGRPGDEWSAGCNSLIRC
                     NKAALVEKSAHIEYLLGWKPASLSAPTQPVLFTNLTDNEKKICEALKSEGGMTIDMLA
                     RLIDIPVYRLSPTLLQMELAGMIILCPGNVYRLRG"
     gene            complement(3275..4612)
                     /gene="gdhA"
                     /locus_tag="PLA17_07820"
     CDS             complement(3275..4612)
                     /gene="gdhA"
                     /locus_tag="PLA17_07820"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPK85360.1"
                     /translation="MKVKEILADLEKKHPGEPEYHQAVKEVLESIEDVYNENPRFAKA
                     GVLQRLVEPDRIFTFKVPWMDDQGNVHVNIGYRVQYNGAIGPYKGGIRFHPTVNLSIL
                     KFLGFEQIFKNSLTSLPMGGGKGGADFDPRGKSENEVMRFCQSFMLELWKHIGPETDV
                     PAGDIGVGAREVGFMYGMYKKLVNEHTGVFTGKGLNWGGSLIRPEATGFGAVYFVKEM
                     LDTVNDSLKGKTVAISGFGNVAWGATLKATQLGAKVVTISGPDGYVYDPAGLSGEKID
                     YMLELRASNQDIVEPYAKKFAGSKFVKGKKPWEVKVDIAMPCATQNELNGEDAKTLVA
                     NKVQLVAEVSNMGCTPEAIAEFQKNKVHFAPGKAVNAGGVATSGLEMTQNSMKLNWNK
                     EEVDEKLHTIMVNIHAACVRNGKREDGYIDYVKGANIAGFLKVANAMVDQGLV"
     gene            complement(4725..5207)
                     /locus_tag="PLA17_07825"
     CDS             complement(4725..5207)
                     /locus_tag="PLA17_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85361.1"
                     /translation="MTIDNGKKIVSLRLTGFIATVIYVLYIFIAYFPKVFRHVMTERS
                     LTTMTIVVTVAYLFLLLLPAIMKYRYIFFSADERTITLRWYKTGLIPGESMSIEIPVN
                     RYAGYEITRQAMGLVHYLTLFQQVQGRKASYPPVSITALSGAQRREIEKTLKGYRSIG
                     "
     gene            complement(5368..5907)
                     /locus_tag="PLA17_07830"
     CDS             complement(5368..5907)
                     /locus_tag="PLA17_07830"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPK85362.1"
                     /translation="MEHGSDEYYMKMALAEAEKAAARDEVPVGAVVVAGGTVIARAHN
                     LTETLNDPAAHAEMQAGSVTAAGGTVIARAHNLTETLNDPTAHAEMQAITAAASYLGG
                     KYLTGCTIYVTVEPCVMCAGALAWSQVSALVWGASDPKKGYTTIGAALLHPKTVVRSG
                     VLARECSEMMKKFFRKKRK"
     gene            complement(5949..6803)
                     /locus_tag="PLA17_07835"
     CDS             complement(5949..6803)
                     /locus_tag="PLA17_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPK85363.1"
                     /translation="MTTSLKTRTARLSVISNSLLIVMKLAAGIISGSVSIISEAIHSS
                     LDLVAAIIAFFAVKISGTPADERHPFGHGKVENVSGVIEALLIFAASAWIIYEAIDRF
                     VHPGETGALGLGVLVMAISAVVNIIVSRRLYKVARQTDSVALEADALHLKTDVITSAG
                     VALGLVLIWLTDWHVLDPIIALVVAVIIIYESWMLLRKAFYPLLDTSLSPSEMKKLTE
                     ILDEMQVSYHDLKTRSAGHQRFVEFHFDVPAHDTIETIHRQCDLIEERLKREFTDIEV
                     IIHPEPTH"
     gene            6923..>8082
                     /gene="aspS"
                     /locus_tag="PLA17_07840"
     CDS             6923..>8082
                     /gene="aspS"
                     /locus_tag="PLA17_07840"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPK85364.1"
                     /translation="MYRTHTCGELSLANRGEKVALAGWVQRSRDLGGMTFIDLRDRYG
                     ITQLVFNMETDADLCMKARKLGREFVIRAEGIVAERSNKNLKIPTGEIEIITEQIEVL
                     NTSDIPPFTIEENTDGGDELRMKYRYLDLRRQNVRSNIILRHKMAQEVRRYLDGNGFI
                     EVETPVLIKSTPEGARDFVVPSRLHHGEFYALPQSPQTFKQLLMVAGFDKYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVHQEDILNMFEGLARHLFHEIKGLEFHGPFPRLTFD
                     EAMSRYGSDKPDIRFGMHITEITEKVKGRNFPPFESADYVGAVSAPGCAGWSRKQLDE
                     LTEFVRRPQIGAKGVVWAKVEADGTVKSSADKYYSANDLANIALMTGSDAGDL"
BASE COUNT         1908 a         2050 c         1988 g         2036 t
ORIGIN      
        1 agcctgtcgc ccagaccggc ttgtttttca ttataccagt caatagcctg agcaatgtca
       61 ttaaagaaac cggggttgta ttctaccgtg aatttcattc gcgggcagac attttacgtt
      121 caatgtcatt ccatgaaaga tagctgtcag gactattttc atattttttt atccgttccc
      181 gtactatttg tttgtgctct tccgggatta caatatcgtc aatgtcttgt tccatccata
      241 aaagttcact aagcatctgt ttttcggaag gagacaattg ctttactata tcaacgacct
      301 gcttgaaact aagtgatata tttaattgcc catgttgagc agccatagtt attatatttt
      361 agccttagta ataaacgaat tatacaataa taaacaataa tagtgacaat tccccggacg
      421 agcttaccat tttttcgata aaaggtaaac atgttgatct cagttttagc ggtgactgtc
      481 tgagcggcga tggtgggttg ttgctacttc ctgattcttt cagcggagag agggggattc
      541 gaacccccga tacccttttg aggtatactc gctttccagg cgagccagtt cagccactcc
      601 tgcatctctc caaaaacggc gcaatttaca aaaattttta cgatgacatc cgggtcattt
      661 caattcgctt ccggcatatc aggcctctgc ccgcaatgca ccggcagatt ctgccgtatg
      721 ctgccgtcct cagctcatct caccgcaaat agacatggag aggagattgc tgacctttcc
      781 ttccggtaac cctctgccaa gcagtaccat catctttgtc acggcagctt cggttgtgag
      841 gtcgcggcca ctgatcacgc cggcactgat cagtcctgcc gaggtcttat acagacccat
      901 ctccacactg ccggcctgac attgtgtaac gttcagcaca attcctcctc cggagatgaa
      961 tgaccgcaat tcttccagga accaccctga cataggagca ttgcccgtac cgtaagtctc
     1021 aagaatgata cccttcaggc cggcctgtgt cagcagggta tgcaccgttt cctgcctgat
     1081 accggggaat agtctcagca cagcaacggc agtctcgata ttatctgaaa ccatcaatgg
     1141 cctgcgtacc gtaggatgcc tgatgatgct cctgttatag cgtatctgca gccctgcctc
     1201 tgcaaggggc ggcaggttgg gtgaggcgaa ggcgttgaaa tgctcggcac tgtacttgat
     1261 tgttctgttg ccacgaaaca acctgctccc aaaaaagatg cacacctccg gtaccatcgg
     1321 caggccatct cccgtggctg ctgctatctc tatggcagtc agaagatttt cctttccgtc
     1381 agtgcgcagg acacctatcg gcagttggga accggtgaag ataacaggct tttcaagtcc
     1441 ctgaagcatg aagctgagag ctgaggctga ataggacatt gtgtctgttc cgtgaagcac
     1501 cacgaagccg tcgaatctgg catagttttc ttcaatggtt ttcgcaatgt ggacccaggt
     1561 atcggggctg acctccgatg aatcgataac cgggtcgaag gtaattgtct caaggctgaa
     1621 tccgaaacgg cgcagctcgg gtacctgatt ggatatgtgc ctgaaatcga ttggcaccag
     1681 tgttcctgat acgggatcat ttaccattcc tatggtgccc ccggtataaa ttatcagtat
     1741 ggatctgttt ttttccatca tttacaggtt aaagagtctc cttgcatttt ccgttgttcg
     1801 cctggcaata gcctcaggtg aaagggaaag tattgtagaa agcctgtcaa tcacataagt
     1861 caggaaggaa gcttcgtttc ttttcccgcg gtgcgggaca ggcgcaaggt aaggggcatc
     1921 ggtttcaagc acaatgttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2041 aacagaaatt ttcctaactt agagctgtct cgctaaagca ttgcataatg tcattgatgc
     2101 cacttgttta caaaattgct cttggcatga tacccggtgt gggcgacatt accgcccgca
     2161 ggctggtttc atacgccggg ggtgtggaag ccgtcttcag ggagtcattc cgcagtctga
     2221 ccaggatccc gggaataggc gaggggcttg catccgccat tgccggtcac gggtatcttc
     2281 ctgccgccga acgggaagca gaatttgtca ccaggaacaa tatcagggtt tacttctacc
     2341 ttgacgatga ttaccctgtg aggctcagac agtgcgaaga ctctccggtg accttttatt
     2401 tccggggcaa cgccgatctt gactcaccga tgatactcag cgtggtaggc acacgacatg
     2461 ccactgagaa cggccgcaga ctgtgccaaa aaatcgtttc cgggctggcg gagcacttcc
     2521 ccggattgct gattgtcagc ggccttgctt acgggattga catcactgca cacagggctg
     2581 ccctcacggc cggactgcct accgttgcag tactgggtca cggactgaag acagtctatc
     2641 cgtcggttca tgccggcgtg gcaaaagcaa tgctcgaaaa gggcgggcat gtcacagact
     2701 tctcctcacc gacgttgccg gagcgtaaca acttcatcag gcgcaaccgc ataattgcgg
     2761 gtatctctga cgccaccctg atcattgagt cagggataaa aggaggggcg cttatcacgg
     2821 ctgacatagc ggcctcttat aaccgtgacg tgatggcggt tcccggcagg ccgggagatg
     2881 aatggtcagc cggatgcaac tcgctgatca ggtgcaataa agccgcactg gtggagaaga
     2941 gtgctcacat tgaatacctt cttggctgga agccggcatc cctgtcagca ccaactcaac
     3001 cggtgctttt caccaatctt acggataatg agaaaaaaat ctgcgaggca ctgaagagtg
     3061 aaggcggtat gaccattgat atgcttgccc ggttgattga tattccggtt tacaggttgt
     3121 cacccacact gctccagatg gagcttgccg ggatgataat cctctgcccg ggaaatgttt
     3181 accggttgcg gggataatga tatggcagat acattcccta tatacacgta acgccaggtc
     3241 tcagacctgg cgttatcatg tcatcacatt atcattacac caacccctgg tcaaccattg
     3301 cgttggcaac cttcaggaat cctgctatgt tggcaccctt cacataatcg atataaccat
     3361 cctcgcgctt gccattcctg acacatgcag catggatatt gaccatgatg gtatgcagct
     3421 tctcgtcaac ctcttccttg ttccagttga gcttcattga gttctgcgtc atctcaaggc
     3481 ctgaggtggc tacacctcct gcattaactg ccttaccggg agcgaagtgt actttgttct
     3541 tctggaattc tgcaatagct tcaggtgtac aacccatgtt agacacctcg gcaacaagct
     3601 gtactttatt ggcaacgagg gtcttggcat cttcaccgtt aagctcgttc tgtgttgcac
     3661 acggcatggc gatgtcaacc ttgacttccc atggcttctt gcctttaacg aacttcgagc
     3721 cggcaaactt cttcgcataa ggctcaacta tatcctggtt ggatgctctc agttcaagca
     3781 tgtagtcaat cttctcaccg ctgagacctg ccgggtcata aacataacca tcaggcccgg
     3841 agattgtaac aaccttggca ccaagctggg tggccttaag ggttgcaccc catgccacgt
     3901 tgccgaaacc ggatattgct accgtcttac cctttaatga gtcattgacc gtgtccagca
     3961 tctccttaac aaaatagaca gcaccgaagc ctgttgcttc aggacggatg agtgagccgc
     4021 cccagttcag acccttgccg gtaaatacac cggtgtgctc attaaccagt ttcttgtaca
     4081 tgccgtacat gaaaccaacc tcgcgggcgc caacacctat atcacctgcg gggacgtctg
     4141 tctcaggacc gatgtgcttc cacagttcaa gcatgaacga ctggcagaaa cgcatcacct
     4201 cgttctctga cttgcccctg ggatcgaagt ctgctccgcc cttgccgcca cccatcggga
     4261 gtgaggtcag ggagttcttg aagatctgct cgaagcccag gaatttgagg attgaaaggt
     4321 tcaccgtagg atggaaacga ataccaccct tgtatggccc aatggctccg ttatactgta
     4381 ccctgtaacc aatgttgacg tggacattac cctgatcatc catccagggc accttgaagg
     4441 tgaaaatacg gtcaggctca accagcctct gaagaacacc tgcctttgca aatcgcgggt
     4501 tctcattgta cacatcctcg atagattcaa ggacctcctt gactgcctga tgatactcgg
     4561 gttcgccggg atgcttcttc tcaaggtcag ccaaaatctc ttttactttc attttatagt
     4621 gttttttatg gtttatgatt ttaagaaaga ctaaattaat atacaaaggt catcgaacct
     4681 gcattcatga aaaatgactt tgctcatgtt ttattttgcc gcaactaacc aattgatcta
     4741 tagcctttaa gggtcttttc gatttccctt ctctgggcac cgctcagtgc cgtgatgctg
     4801 acaggcgggt aagatgcttt tcttccctgt acctgctgga agagtgtcag ataatggacc
     4861 agtcccatgg cttgcctggt tatctcataa ccggcatatc tgtttacggg tatctcaata
     4921 ctcatgctct cgccgggtat cagcccggtc ttgtaccagc gaagggtgat ggtccgctcg
     4981 tcagctgaaa agaagatata cctgtatttc attatggccg gcagcaggag caaaaacagg
     5041 tatgccaccg ttaccacaat tgtcattgtt gtaagacttc tttctgtcat tacatgtctg
     5101 aataccttcg ggaaatatgc gatgaagatg taaagcacat agatcacagt tgcgataaag
     5161 ccggtcagcc tcagcgaaac aatttttttg ccgttgtcaa tggtcatgat cagttcgttt
     5221 taagcaaacc tatgaatttt ttccagattc ttcatgtgcg acaggcaaca agattgcctg
     5281 tgcgatagtg cataatagtc tttctttcat ccggcgggcg gctttttccc ccggatcatt
     5341 gattcgctcc tcatccggag cccgctatca ttttctcttt ttcctgaaga actttttcat
     5401 catctccgag cactcccggg caagcacgcc ggagcgcacc accgtcttcg gatgcaacag
     5461 tgcagcacca atggtggtat agcccttttt cgggtctgaa gcaccccaga ccagtgctga
     5521 gacctggctc catgccaacg caccggcgca catgacgcat ggctcaacgg tgacgtaaat
     5581 ggtacagccg gtgaggtatt tacccccaag gtaagaagca gcggctgtga tggcctgcat
     5641 ctcagcatgg gcggtgggat cattgagtgt ctccgtcagg ttgtgggcac gggcaatcac
     5701 tgtgcctccg gcagctgtca ctgaccctgc ctgcatctca gcatgggcgg cgggatcatt
     5761 gagtgtctcc gtcaggttgt gggcacgggc aatcactgtg cctccggcaa ctaccactgc
     5821 ccccacgggt acctcatcac gggcagcagc cttttcggct tcggcaagcg ccatcttcat
     5881 gtaatactcg tcgcttccgt gttccatggt gatattctct cttcagctta cggggaattg
     5941 cagcgttatt aatgtgtcgg ttcaggatgg atgatcactt caatatccgt gaattcgcgc
     6001 ttcaggcgct cctctatcag gtcacactga cggtggatgg tttcaatcgt atcgtgagcc
     6061 ggcacatcaa agtggaactc aacaaacctc tggtggccgg cactccttgt cttcaggtca
     6121 tggtagctga cctgcatctc atcaagtatc tctgtcagtt tcttcatctc agacggtgag
     6181 agggatgtgt caaggagcgg atagaaagct tttctgagaa gcatccatga ttcatatatt
     6241 attattacgg ccacgaccag ggcgatgatg gggtcaagca catgccagtc tgtcagccag
     6301 atcagcacca gaccgagcgc cacaccggcc gatgtgatta catcagtctt gagatgcagg
     6361 gcgtcagctt ccagggcaac ggagtcggtc tgccgggcaa ccttgtacag gcgcctggag
     6421 acaatgatgt tgacaacagc agagatagcc atcaccagca cacccaggcc cagcgccccc
     6481 gtctccccgg gatgcacaaa cctgtcaatg gcctcatata taatccatgc cgaggcggca
     6541 aaaatcaaca gtgcctcaat cacgccggag acattctcca ccttgccgtg gccgaaggga
     6601 tgtctctcat cagcaggtgt ccccgatatc tttactgcga aaaatgcaat aatggctgcc
     6661 accaggtcca gggaagaatg gatggcctcg gaaatgatgc tgaccgaacc ggaaataatc
     6721 cctgcagcca gtttcatcac tataagcagg ctgttcgata tgactgaaag ccttgcagtt
     6781 cgtgtcttca gtgaggtggt cattgctgtc ggaaccgctt ttaaactatt gcaattttag
     6841 gcaaaatgag tgacaagtca gaggaaagtt attaccgttt gttaccggaa tgctttattt
     6901 ttgtctcctc aagataactg atatgtacag gacacatact tgcggtgagc tttcactggc
     6961 caacagggga gagaaggtag ctctggccgg atgggtacag cgctcaagag acctcggggg
     7021 aatgacgttt attgacctgc gcgacaggta cggtatcacc cagctggtct ttaacatgga
     7081 gaccgatgct gacctttgca tgaaagcccg taaactggga cgcgagtttg taataagggc
     7141 tgaaggcatt gtcgctgagc gaagcaataa aaacctgaag atccctacgg gcgaaataga
     7201 gataatcact gagcagatcg aagtgctcaa tacatctgat atcccgccct tcactattga
     7261 ggagaacacc gatggtggtg acgagctgag gatgaaatac aggtaccttg acctgcgaag
     7321 gcagaacgtg cgctcgaaca tcatcctcag gcacaaaatg gcacaggaag tgcgcaggta
     7381 cctcgacggc aatggattca tcgaggtgga gacaccggtg ctcatcaagt caacccccga
     7441 gggggcacgc gattttgttg tgccttcaag gctgcaccac ggcgagtttt atgcgcttcc
     7501 ccagagtccg cagacattca agcagttgct gatggtggcc ggattcgaca agtatttcca
     7561 gattgtaaag tgcttccgtg atgaggacct gcgtgcagac cggcagccgg aattcaccca
     7621 gatcgactgc gagatgtcat ttgtccacca ggaagatatc ctgaatatgt ttgaagggct
     7681 tgcccgccat cttttccatg agataaaagg gctggagttt cacgggcctt tcccgcgatt
     7741 aacgtttgat gaggccatga gccggtatgg cagtgacaag ccggatatca ggttcggcat
     7801 gcacattact gagattactg agaaggtaaa gggtcgtaat ttcccgcctt ttgaatctgc
     7861 tgactatgtg ggggctgttt ccgcaccggg ttgtgcaggc tggtcacgca aacagcttga
     7921 tgaactgact gagtttgtca ggcgtccgca gataggggca aagggagttg tctgggcaaa
     7981 ggttgaggca gacggaacgg tgaaatcgtc ggccgacaaa tactatagcg ctaatgacct
     8041 tgcaaacata gcgctgatga ccggttcgga tgccggcgac ct
//
LOCUS       DAOPBK010000121         8017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8261_length_8017_cov_4.686762, whole genome shotgun sequence.
ACCESSION   DAOPBK010000121 DAOPBK010000000
VERSION     DAOPBK010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8261_length_8017_cov_4.686762"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="PLA17_07845"
     CDS             complement(<1..1388)
                     /locus_tag="PLA17_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HPK85365.1"
                     /translation="MHDILELSEMLVPELREIARQLKIKRVELLKKQDLIYKILDQQA
                     IDAAETRKTGRAEKQPSDKKQDQSGQQKKQRKPDKTTKSADQRRQQGQKEQQQGQQQQ
                     QQQQQQQQPRQQDQEQPPKSQHEQQQMKSQDQKEQAQKEQDKQQQNRQQQWQQNRDKR
                     PRTFRNDQQGQHRHDQSRQQQKARHDEMVQTPSRQPQQQNREQSYQNEHGVPPADKLL
                     LTRETGTDNDHYLLRLPESEGESRIIGEAAAPDGVVVIPPATAEATLSQPEVHEPVKE
                     EKKEKIYDFEGIVSNTGVLEIMPDGYGFLRSPDYNYLSSPDDIYVSQSQIKLFGLKTG
                     DTIRGTIRPPREGEKYFPLIKVEEINGRSPDFIRDRVPFDFLTPLFPNEKFTLCRPGE
                     GNLSVRIVDMFCPIGKGQRGLIVAQPKTGKTILLQDIANAIAKFHPEVYLMILLIDER
                     PEEVTDMARNVNA"
     gene            1724..2587
                     /locus_tag="PLA17_07850"
     CDS             1724..2587
                     /locus_tag="PLA17_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPK85366.1"
                     /translation="MRQLKITKQVTNRDTPSLDKYLHEIGKVELISPDEEVMLARAIR
                     AGDHDALARLVKANLRFVVSVAKQYQNHGMTLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQAILQSLAEQSRIVRLPVNKIGSINRINKAYSKLEQEFEREPSAQEIAE
                     LLEIAPEDVKETIRTNGRTLSMDAPLSAEDDNNMYDMMQNEETPSPDRNLINESLSYE
                     IEKALSTLSPREARVLKLYFGIGMKHPFTLEEIGEELKLTRERVRQIKEKAIKRIQFT
                     TRSRILKSYLG"
     gene            2613..3266
                     /gene="rpe"
                     /locus_tag="PLA17_07855"
     CDS             2613..3266
                     /gene="rpe"
                     /locus_tag="PLA17_07855"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPK85367.1"
                     /translation="MSHLVAPSLLAADFANLASEVEMINDSEADWIHLDIMDGLFVPN
                     ISFGFPVVEAVSDLASKPLDVHLMIVDPDRYLERFRDAGASYLTVQFEACNHLHRTVT
                     EIRRLGMKAGVALNPHSPVILIKDILPYIDMVLLMTVNPGYGGQTFIPGSINKIGELR
                     SMIDEGGYEVLIEVDGGVDLHNAPMLVHAGVDVLVAGSTVFSSSDPAETIRRLREPV"
     gene            complement(3318..4223)
                     /gene="miaA"
                     /locus_tag="PLA17_07860"
     CDS             complement(3318..4223)
                     /gene="miaA"
                     /locus_tag="PLA17_07860"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPK85368.1"
                     /translation="MKNTLIVLPGPTGVGKTAFATGLAERLGCDIISCDSRQFYREMK
                     IGTAAPGEEELARAKHHFVGFLSVTDYYSISLFERDVLALLPSLFPSNPVVIMTGGSM
                     LYMDAVCRGMDDIPDTDPAVRQKYLEMYHNEGIEGLRIALKLLDPVSYSRIDLHNPRR
                     ILRALEITESSGRPYSSFLTAPVRQRDFRIIKAGLTRERSELYRRIDMRVDRMMEEGL
                     EQEAASLIRYRGLNALNTVGYREMFRWFDGEVSREEAVMLIRRNTRRYARKQLTWWNR
                     DSQINWFDASESEKIISWIEKSLDE"
     gene            complement(4245..4820)
                     /locus_tag="PLA17_07865"
     CDS             complement(4245..4820)
                     /locus_tag="PLA17_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85369.1"
                     /translation="MVYRFVVLSDEDETFVMEFEFLDSQNLLDFHNILQDELEFDRSQ
                     MASFFMATESWEKEEEFTLFDIGGGSSTMENAVLEEIIFRKNQKLLYVFDFFNDRALY
                     IEYVGEFEEEDDKEYPVCTTSKGMPPKQVTFGSVLRKKYNNLVVTDDDDDDDVVDEEV
                     VDDDIFFSGDDEDDLSDFDNIGHGDEDDEED"
     gene            complement(4879..5919)
                     /locus_tag="PLA17_07870"
     CDS             complement(4879..5919)
                     /locus_tag="PLA17_07870"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HPK85370.1"
                     /translation="MKNDHLSVERGIEAFAELGRAMREGAAGTGTGSGKLFHELSGRL
                     CLSNPWFTPGNVRRALHATGAALTKEKLTRWLSAYPELNDKRDPVTVGVVMAGNIPMV
                     GFHDLLCVLISGNRLLARVSTKDEILMRAVAATLAEIEPAFGNVTEITTGQLKGFGKV
                     IATGSNNTSRYFEYYFRNVPSIIRKNRNSIAILDGTESDSELELLGDDIFSYFGLGCR
                     NVSKLYLPRGYDPASLLPRWDRHESLLSHYRYMINYDHHRAVMIVNRESFTDGRFILL
                     KESSSLTPPMAVIHYEFYDSSTGPAITPEMSEEMLQCIVGHDHLPFGKTQEPEVWDYA
                     DNIDTISFLLKK"
     gene            6020..7252
                     /locus_tag="PLA17_07875"
     CDS             6020..7252
                     /locus_tag="PLA17_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK85371.1"
                     /translation="MTLFWENVRVAFKAIRSNRVRAALTMAIIAFGIMALVGILTAID
                     GLKSTLTDQFTLMGANSFSITSRSMRVTVGNQHLRTRNHSHITFKEAMAFKERFQEPA
                     WVSISFSASGMAEYRFGNNKTNPNISILGSDENYLTFAGQELESGRNFSQDDLSGSRH
                     VVIIGKDIVRFLFPGVDPLGKEISLPGLKLTVIGVIKSSGASMMGTDQMTIIPITTAR
                     QYFSRPNISYNIGVMPHKSVSLDMMASEAEGIFRIVRNLDPRDESDFNIQKSDSLIAM
                     LLENIRFVTLAATIIGFITLFGAAVGLMNIMLVSVTERTREIGVRKALGARKSVIRQQ
                     FLYESVIIGQLGGFFGIIAGILIGNLVSFGMGSSFTVPWVWVIGGVMACFLVGIASGY
                     IPAVKASAVDPIEALRYE"
     gene            7303..7680
                     /locus_tag="PLA17_07880"
     CDS             7303..7680
                     /locus_tag="PLA17_07880"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPK85372.1"
                     /translation="MSVEVILAGSNNIPRITYDQDEDLLSITGRSIAERSDIIYTPLE
                     QWIRSHLERFTTLRIVIFLEYINSGSSKAMRDVLRMLSNYRASRYRIRITWLYEEDDE
                     SMYELGEHYRDSLGIDMEVKMVL"
     gene            complement(7835..>8017)
                     /locus_tag="PLA17_07885"
     CDS             complement(7835..>8017)
                     /locus_tag="PLA17_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPK85373.1"
                     /translation="EYTEDEIVSVDVIHNPASAVFDAKSTMVNGKMIKVLAWYDNEWG
                     YSARCVDLIEMMGKTL"
BASE COUNT         1906 a         1975 c         1999 g         2137 t
ORIGIN      
        1 gcattcacat tgcgggccat gtcggtgacc tcctcgggac gctcgtctat cagaaggatc
       61 atgagataga cctccgggtg gaatttggca atggcattgg caatatcctg caggagtatg
      121 gtcttgccgg tctttggctg cgccacgata agaccgcgct gtcccttgcc tatggggcag
      181 aacatgtcca cgatgcgtac tgacaggttt ccttcacccg gcctgcagag cgtaaatttt
      241 tcattgggga agagcggagt aaggaaatca aagggtaccc tgtcacggat gaaatccggt
      301 gaacggccat tgatctcttc aaccttgatg agagggaagt atttttcgcc ctcgcgtggc
      361 ggacggatgg ttcccctgat ggtgtcaccg gtcttcaggc cgaagagctt tatctgcgac
      421 tgtgatacgt atatgtcatc aggtgaactg agatagttgt agtcaggcga gcggaggaac
      481 ccgtaaccgt cgggcattat ctccagcaca cccgtatttg atactattcc ttcgaaatca
      541 tagatttttt ctttcttttc ctctttgacc ggctcatgca cctcgggctg tgacagagtg
      601 gcttcggctg tagccggtgg aatgaccaca acgccatcag gcgctgctgc ctccccgatg
      661 atccggcttt caccttcaga ttcagggagc ctcaggagat aatgatcgtt gtcagtgcct
      721 gtttcccggg taagtagaag cttgtcggca ggtggtactc catgttcatt ctggtagctt
      781 tgttcacggt tctgttgctg tggctgccgg gatggtgtct gcaccatttc gtcatgcctg
      841 gccttctgct gctggcggct ttggtcatgc ctgtgctgtc cctgctggtc attcctgaaa
      901 gtacgtggac gcttgtcgcg attctgctgc cactgctgct gcctgttctg ctgttgctta
      961 tcctgctcct tctgagcctg ctctttctgg tcctgggact tcatttgctg ctgctcatgc
     1021 tgcgatttag gaggctgttc ctggtcttgc tgcctgggct gttgctgctg ctgttgctgt
     1081 tgctgttgct gttgcccctg ctgctgctcc ttctgaccct gctggcggcg ctgatcagca
     1141 ctcttcgtgg ttttgtcagg ttttctttgc ttcttttgct gaccggattg atcctgtttt
     1201 ttgtcagaag gctgtttttc agctctgccg gtcttgcgtg tctcagcagc atcaatggcc
     1261 tgctgatcaa gaattttgta gataaggtcc tgcttcttaa gtagttccac cctcttaatt
     1321 ttgagctgcc tggcaatctc cctcaattcg ggaaccagca tctcgctcag ttcaagaata
     1381 tcatgcataa tgtgataata tgtagaagaa ttatattgat tgtttttgat gaagcgggga
     1441 atttgaaaga ttccctttga atgagtaaat gatctgagtc attatttcgg tgcaattata
     1501 agtaataatt acttaatata caaattgcag cacaaaaagt gaaacacggt tcttcctgat
     1561 cttttatctt ttccaacgac caatggtgaa tggttgaaaa aataccttta caggggacat
     1621 tattattaaa atctacacaa ctcttgttta tctgagaata ctttagtaaa attgtaagct
     1681 tcagggagaa agaaacggta gtaagttcgc aaattacttg taaatgcgtc agttaaagat
     1741 aaccaaacag gttaccaatc gtgatacccc ttctcttgat aagtacctgc atgagatagg
     1801 gaaggttgag ttgatctcac ccgatgagga ggtgatgctg gcgcgtgcca tacgtgcagg
     1861 tgaccatgac gccctggcaa ggcttgtgaa agccaacctg cggtttgttg tttccgttgc
     1921 aaaacagtac cagaaccatg gtatgacact tcctgacctc attaacgaag gaaacctcgg
     1981 cctcatcaag gctgcccagc gttttgacga gaccaggggc ttcaaattca tttcatacgc
     2041 tgtctggtgg atcaggcagg ccatccttca gtcacttgcc gaacagtcca ggattgtaag
     2101 gctgccggtc aacaagatag gctccatcaa tcgcatcaac aaggcctact ccaagcttga
     2161 acaggaattt gaacgggagc cttcagcgca ggagattgcc gagctgcttg agatagctcc
     2221 ggaggatgtc aaggagacca tacggaccaa cggacgcaca ctgagcatgg atgccccgtt
     2281 aagtgctgag gatgacaaca acatgtatga catgatgcag aatgaggaga cgcccagccc
     2341 tgaccgcaac cttatcaatg aatcgttatc ctatgaaata gaaaaagccc tcagtactct
     2401 gtcgccccgt gaggcgaggg tgctgaagct ttatttcggc atcgggatga aacacccatt
     2461 cacccttgaa gagataggtg aagaactgaa gctcacccgt gagcgggtaa ggcagataaa
     2521 ggaaaaggca ataaagcgca ttcagttcac cacccgcagc cggatactca agtcatacct
     2581 gggctgatca caaaactgat attcttagac ttatgagcca ccttgttgca ccgtcactac
     2641 ttgccgctga ctttgccaac cttgcatcgg aggttgaaat gattaatgac agcgaagccg
     2701 actggattca tcttgacata atggacggtc tgtttgttcc caacatttca tttggttttc
     2761 cggtagtgga agcggttagc gacctggcat caaagccgct tgacgtacac ctgatgatag
     2821 ttgatcctga ccggtatctt gaaaggttca gggatgccgg cgcctcatac ctgactgtac
     2881 aattcgaggc atgcaaccac cttcaccgga ccgttaccga gatacgcagg ctcgggatga
     2941 aggcaggggt agcactgaat cctcattctc cggtgattct cataaaggat attcttccat
     3001 atattgatat ggttctgctg atgacggtca accccggcta cggcgggcag accttcattc
     3061 cgggcagcat caacaagata ggggagctca gaagcatgat tgatgaggga ggctacgagg
     3121 ttttgataga ggtagacggc ggtgtggatc tgcataatgc ccccatgctt gtgcatgccg
     3181 gtgtggatgt gcttgttgca ggcagtacag tattctcttc atcagaccct gctgagacaa
     3241 tcagaaggct cagggagccg gtctgatccc gcaagctcaa ccgccacgtt attattaagg
     3301 agggccaccg ggcctggtta ctcatccagg ctcttttcaa tccatgagat gatcttctca
     3361 ctctccgacg catcgaacca gtttatctgg ctgtccctgt tccaccaggt aagctgtttt
     3421 cgcgcatagc gccttgtgtt tctcctgatg agcatcactg cctcttcacg tgagacctct
     3481 ccgtcaaacc atctgaacat ttcgcggtag ccgacggtgt taagggcatt cagccccctg
     3541 taccgtatca gtgaggcagc ttcctgctca agcccctctt ccatcatgcg gtcaaccctc
     3601 atgtcaatcc tgcggtacag ttcgcttctc tcccgtgtca gtccggcttt gatgatcctg
     3661 aaatcgcgct gcctcaccgg tgccgtgagg aacgaagagt atggtctgcc ggagctttcg
     3721 gttatctcca gggcacggag tatccggcgt ggattgtgaa ggtcgatcct tgaataggat
     3781 accggatcaa gtaatttaag tgcaatgcgc aggccctcaa tgccttcatt gtggtacatt
     3841 tcaagatact tctgcctgac ggcaggatca gtatcaggga tgtcatccat gcccctgcat
     3901 accgcatcca tatagagcat tgaccctccc gtcatgatta ccaccgggtt cgacgggaac
     3961 aatgatggca gaagggccag cacatccctc tcgaacaggc tgatgctgta ataatcggtg
     4021 acagacagga atcccacgaa gtggtgtttt gcccgcgcaa gttcctcctc gccgggggca
     4081 gccgttccga tcttcatctc gcggtagaac tgccttgagt cacatgaaat aatatcacag
     4141 cccagccttt cggccagccc ggtggcaaat gccgttttgc caacaccggt gggaccggga
     4201 agaacaatca gtgtattttt catggagtag gggtgaaggg gcaactaatc ctcttcatca
     4261 tcctcgtcac cgtgtccaat gttgtcgaaa tcagacagat catcttcatc gtcaccactg
     4321 aagaagatat cgtcatccac aacctcctca tcaaccacat cgtcatcatc gtcatcatcg
     4381 gtgacaacca ggttattata ttttttgcgc aaaacgctgc caaaagtcac ctgcttgggg
     4441 ggcatgccct tggaggtggt gcataccgga tactctttgt catcctcttc ctcgaactca
     4501 cccacgtatt cgatgtagag tgccctgtca ttgaaaaagt caaaaacata gagcagtttc
     4561 tggtttttgc ggaagatgat ctcttcaagc acagcgttct ccattgttga tgagcctccg
     4621 cctatatcaa acagtgtaaa ctcttcctcc ttctcccagc tctctgtagc cataaaaaaa
     4681 gaagccatct gggaacggtc aaactccagc tcatcctgca ggatattgtg aaaatcaagc
     4741 agattttgac tgtcaaggaa ttcaaactcc attacaaatg tctcatcctc atcagataat
     4801 accacgaacc tgtaaaccat aacactaact ttttcaactg gtgcaattta atatttttta
     4861 taatagattc actctgactt attttttaag caggaaactg attgtgtcaa tattgtcagc
     4921 gtaatcccac acttccggtt cctgggtttt cccgaaaggc agatgatcgt gcccgactat
     4981 gcattgcagc atttcttcac tcatttccgg ggttatggcg gggcctgttg agctgtcgta
     5041 gaactcataa tgaatcacag ccatcggggg agtgagtgat gacgactctt taagcagaat
     5101 gaagcggccg tcagtgaaac tttccctgtt aactatcatc actgccctgt gatggtcata
     5161 gtttatcata tacctgtaat gcgacagcag tgattcatgg cgatcccagc ggggcaggag
     5221 ggatgccggg tcatatccgc gggggaggta cagttttgat acattcctgc aacccagtcc
     5281 gaagtatgaa aagatatcat cgccaagcag ctccagttcg ctgtcactct ctgtcccgtc
     5341 aagaatggct atactgttcc tgttctttct gatgatggag ggaacatttc tgaaatagta
     5401 ctcgaagtat cttgatgtat tgttgcttcc tgttgcaatc actttcccga atcccttcag
     5461 ctggccggtt gtgatctcag taacatttcc gaacgccggt tcaatttcag ccagtgtggc
     5521 ggccacggcc ctcatcagta tttcatcttt ggtgcttact ctggccagca ggcggtttcc
     5581 tgatatcagc acgcagagca ggtcatggaa tcctaccata gggatgttgc cggccataac
     5641 cacccccacg gttaccggat cacgtttatc attcagctcc gggtaggctg agagccaccg
     5701 ggtcagtttt tctttggtca gggcggcccc ggtggcatgg agtgcacgcc tgacattccc
     5761 gggtgtgaac cacggattgg agagacagag tctcccggaa agttcatgaa agagttttcc
     5821 tgatcccgta ccggtgccgg ccgctccttc gcgcatcgct cttccaagct ctgcaaaggc
     5881 ttcaatgcca cgttcaactg acagatggtc gttcttcacc cgggcaaagg taaaaaatga
     5941 agtgaatgtt taaagataaa gaaattcaga aaagatgtat cttagttgca tctttatctg
     6001 caagaagtaa ttgaagggat tgactttatt ctgggagaat gtcagggttg cattcaaggc
     6061 aatcaggtca aacagggtca gggctgcact gacaatggct ataattgcct tcgggatcat
     6121 ggcccttgtg gggatactta ccgccattga cgggttgaag agcactctta ccgatcaatt
     6181 caccctgatg ggggcaaact ccttctcaat cacctcacgc tcaatgaggg ttaccgttgg
     6241 taatcagcat ctacgcacca ggaaccattc ccatatcacc tttaaggagg ccatggcctt
     6301 caaggagagg ttccaggaac cggcctgggt atcaatatca ttcagtgcat cgggtatggc
     6361 cgaataccgc ttcggcaaca ataaaaccaa tcccaacatt tcaatcctgg gttcggatga
     6421 aaactatctg acctttgccg gacaggagct tgaatcaggg cgcaacttct cacaggatga
     6481 cctgtctggc agcaggcacg tggtcattat cgggaaggat atagtccgct tcctctttcc
     6541 cggcgttgac ccgctcggaa aggagataag ccttcccggc ctgaagctta cggttatcgg
     6601 ggtgatcaag agttcaggtg caagcatgat gggtactgac cagatgacca ttatcccgat
     6661 tactaccgcc agacagtact tctcaagacc caacatttca tacaatatag gcgtgatgcc
     6721 gcataaatcg gttagtcttg acatgatggc aagcgaagcc gagggtatct tcaggatagt
     6781 gcgtaacctc gatccccggg atgagtctga tttcaatata cagaagagtg actccctgat
     6841 tgcgatgctg ttggagaaca tcaggtttgt gacgctggct gccacgatta ttggctttat
     6901 aactctcttc ggagcagctg tggggctgat gaatataatg ctggtatccg ttacggagcg
     6961 caccagggag attggagtcc gaaaggcgct cggggcgcgc aagtcagtga tcaggcaaca
     7021 gtttctttat gaatctgtca ttatcgggca gttaggaggc ttttttggca ttatagcggg
     7081 cattcttatc ggtaacctgg tttctttcgg gatgggttca agtttcaccg tgccatgggt
     7141 gtgggtcatc ggaggtgtga tggcctgttt cctggtaggg attgcttcgg gatacatccc
     7201 cgccgtaaag gcatcggcag ttgacccgat tgaggcgctt cgctatgagt gatataaaac
     7261 ctggttgatg aaccgaactt aattgatgca ggattttgaa aaatgagtgt tgaagtaatt
     7321 ctggcaggat cgaacaatat tccacggatc acctatgatc aggatgagga tctgttgagc
     7381 atcacgggac gttcaattgc tgagaggtct gatattatct acactccgct ggagcaatgg
     7441 atcagatcac atctggaacg tttcaccaca ctcaggattg tcattttcct ggagtatatc
     7501 aacagcggat catcaaaggc gatgcgcgat gtgctcagga tgctcagcaa ctaccgggct
     7561 tcacgctaca ggatacgcat cacctggctc tacgaggagg atgacgagtc aatgtatgaa
     7621 ctgggtgaac actaccgtga ttcgctcggc attgacatgg aagtgaagat ggtgttgtaa
     7681 ccgggggagg gaatcattcc ccggattcgg attatgcata cacatacgca gcgaggggtc
     7741 tcagacccct cgcaacattc tccggacaca tcccccggga ttcagatcgg gaatatatat
     7801 acgtagcgag gggtctcaga cccctcgcta cctgctataa tgttttaccc atcatttcga
     7861 tgaggtcaac acatcttgct gaataacccc attcgttgtc ataccatgca aggaccttga
     7921 tcatcttgcc gttgaccatt gtgctcttgg catcgaacac tgctgatgcc gggttgtgga
     7981 taacatcaac ggagacaatt tcgtcctcgg tgtattc
//
LOCUS       DAOPBK010000122         7996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8283_length_7996_cov_5.336482, whole genome shotgun sequence.
ACCESSION   DAOPBK010000122 DAOPBK010000000
VERSION     DAOPBK010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8283_length_7996_cov_5.336482"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLA17_07890"
     CDS             complement(<1..598)
                     /locus_tag="PLA17_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPK85374.1"
                     /translation="MMDNQDLRLELKAIDTSSLVDKVEMRLIEVFIYKELKPGDAIPK
                     ETELATVMGVSRTVIRESLNRLKTMGLIETKKHRGTIIKSPDLSAVLGKSLIPRILDN
                     QTLMDVFEMRLALEIGMADFIFARKTDSDISELEKIVEIEPDHSDNILFDVDHELRFH
                     GKLYQMAGNNTLEEFQNLLLPAFQHVYNIGLIKVKGKRK"
     gene            1405..2826
                     /locus_tag="PLA17_07895"
     CDS             1405..2826
                     /locus_tag="PLA17_07895"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPK85375.1"
                     /translation="MVVNFSPGSDIEAVGEVYVKCQASGQAGAVVFGAARAERKVTID
                     GDYQFRAGENLITLGISPSSVSELTDKLEVESVSIILEDGSVLPAPTVSFPRVRFGTV
                     VRAAGQDGADTYRIPGLVTTNEGTLIAVYDIRYNNSKDLQEDIDIGMSRSTDGGRTWE
                     PMKVIMDMGEYGGLPQNLNGIGDPCILYDHTTGTLWVAALWMSGSAPDKMLWRASQPG
                     MTPQETGQFILVKSTDDGLTWSEPVNITSQIKDSAWQLLLQGPGRGITLDDGTLVFPA
                     QFKADIGTKALDGGEYTCHSTIVCSMDGGDTWQIGSGAKSNTTEAQVVQLADGSLMLN
                     MRDDRNRSDKSETNGRAVAVTTDLGKTWRAHPSSNSALPEPNCMASLISAKINIAGDD
                     RDVLFFSNPNDRNNRIRMTIKASTDQGLTWPEENQVELFAPEGYGYSCLTMVDETHVG
                     IIYEGVKELYFQKIPVADLLGDR"
     gene            2845..6801
                     /locus_tag="PLA17_07900"
     CDS             2845..6801
                     /locus_tag="PLA17_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPK85376.1"
                     /translation="MNFPAKEWLLLILISLFTGGIIYPVQLSEKPSLIPAPKIFSLTV
                     EKFRVRQAGLSDDQRIRKVVVPAIEEAPLNIEEAFRIIVRSDSVLIYATGDEGFFRAD
                     ATLKQLAGSDTDGEFIQGCSITDWPAFRIRGFMHDCGRSFIPVSELKREVELLSLFKI
                     NTFHWHLTENLAWRLASDIMPELTDPDVTLRDNHGYYTREEVRDFVRFCRDHFVEVIP
                     EIDMPGHSAAFTRATGHSMQSEEGRKIVVQLLREACNLFEGSYFHIGTDEVNIASPDF
                     VPEMMDIVRSYGKEVIGWLPGAPMDQGALRQLWADVSLPQNTPVIDSRYRYLNHTDYY
                     ADLFSIYNSRMCDAEQGSDLLAGGICCIWNDRKPASVEDILISNAFYPVMLAFAERSW
                     SGGGDDIKSRGVRMGLPGDPAFERFREFEARMISLKERFLNDVPFPYLPQTGILWNIS
                     RQYPNDGNPDYSAPFENDLVTDPDKVSSADAGFRPAAGAAVYLRHTWGEKVPAFYIDP
                     APDHTAYACTWIYSPTDRQAGLHFSTHNYGRSELDMAPPQGEWDYKRSRVWLNGEPVS
                     PPEWNYTGVKPDHETPYTNENMWSRPPAAITLREGWNSLVIKLPVGSFSTPETRLVKW
                     MFTAMIVTPDGSAQPDGLVWSPDKLFPGDKTRIACIGNSVTYGTGVAGRDTNAYPAVL
                     QRMLGGRYIAGNFGRSGATLLKKGYRPYNRTEEFRAALSFRPDIAVIHLGLNDTDPRA
                     WPDFRDEFISDYLWLIDTLRSVGTKEFYIARLSPIFHGHRRFKAGTRDWYWQIQEMIE
                     EVASLSGATLIDFYTPLKARPDLMPDNLHPDVRGAAMLAETVFSALTGNYGGLQLPQA
                     WSDGMVLQHDRVITLKGKANGKEPVEVSLAGRLYQAMTTPTGEWEVKLDPLEAGGPYQ
                     LIAMTRSDTVVIRDILAGEVWFCSGQSNMEWTAGNSDGWQEIATLPPDENLRLITFSR
                     RDLSWNEPWDSVTLDKINNLDYFREGAWRVASPESIKDFSGVAYYFGRKLRDELGVPV
                     GLIEMAVGGSPAEAWIERKALEMHPQLVDLLYDYRKSEFREPWVSNTVTTNLRNSDNP
                     RQRHPFEPAYLYESGISRIAGYPVRGFLWYQGESNAIFSELYEVMLPELARNWRDAWD
                     DDHLPFYFAQLSSLNRPSWPQFRDTQRRLVSEIPNSGMVVTTDAGHPTDVHPRDKKSV
                     GERFASLALNDTYGFRKAPRSPEPLSAERKGDMVIISFSCSDRLKTSDGLPLREIEVS
                     ESGNYFRPATARIRGNRLEINAPGAIAVRYGWKPYSTGNLTGESGLPVSTFRLKIK"
     gene            6806..7726
                     /locus_tag="PLA17_07905"
     CDS             6806..7726
                     /locus_tag="PLA17_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPK85377.1"
                     /translation="MKIKKLAGLIAAPFTPMYPDGEINSGIIEQYHRVLVSNGVAGAF
                     INGSSGEGVSTTSHEKLIVAEAWAKASSGSNLMVISHLGGTCLRECVELARESERMGL
                     YAVSVTSPSYFKPANVKMLAEFFIRVAESVPETPVYYYHIPVLTGGFFNMIDFVREVA
                     PRVPNFAGIKFTHEDFMDFLSCLNYDNGRYDMLWGRDENFLSALVLGARGAVGSTYNY
                     AAPLYHKLIEAFDRNDQVTARAMQQKSIDMITLLGKYGGIATGKAYMRFIGLDCGEFR
                     LPVRNMSEADYQAFVKDVRELGMDDLFSKI"
     gene            7810..>7996
                     /locus_tag="PLA17_07910"
     CDS             7810..>7996
                     /locus_tag="PLA17_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclically-permuted mutarotase family protein"
                     /protein_id="HPK85378.1"
                     /translation="MNAEWTELAPLPPSSPGAIQPGVAGPFTGLHGNTLILAGGANFP
                     DTMPWHGGRKKYHNEIYL"
BASE COUNT         2061 a         1845 c         2090 g         2000 t
ORIGIN      
        1 tcttccgttt cccctttacc ttgatgagcc cgatgttgta aacatgctgg aatgccggca
       61 acagcaggtt ttgaaactct tcaagagtgt tgttgccggc catctggtac agtttgccgt
      121 ggaatcttaa ctcatggtca acgtcaaaca gtatattatc tgagtgatca ggttcgatct
      181 cgactatctt ttcgagttca cttatatcac tgtcggtttt ccgtgcgaat atgaaatcgg
      241 ccatcccgat ctccagtgcg aggcgcatct cgaaaacgtc catcaacgtc tgattatcaa
      301 gtatcctggg tatgagtgat ttgccaagga ctgcggaaag atcggggctt ttgatgatgg
      361 tccccctgtg tttttttgtt tcgatcagac ccattgtttt gagtctgttc agtgattccc
      421 tgatgacagt cctgcttacg cccattactg tggcaagttc tgtctccttg ggtatggcat
      481 caccgggttt aagttccttg tagatgaaaa cctctatgag gcgcatctcg accttatcaa
      541 caagactgct tgtatcaatt gctttcagct caagccttaa gtcctgatta tccatcatat
      601 atcgaatgtg tatttataat agtccggtat attcttcaaa tgattgccaa taactgcctg
      661 attgtcagga ttggtcaatt tctttaaata aacattctaa ttaattgatt atgtgtttat
      721 tggcaaaaat ttacaacctg ttctatttag aaatagcaaa agaagcaaaa aaaacggaga
      781 aacccttgca tgtaagagta aaagattatt tttgtattaa aaatatgtat gacataatac
      841 actcgaattt aattcaatat ttactccaaa ccaactcaat tcatgaagaa aaagtcaatc
      901 ccttttgtca cctgatgatg cttttgcctg ctgtattgct cagcggagac tctctgttgg
      961 tccagtgaca atcaggggac ggttacggat ggaacaaccg gtgagctgct gccggtctga
     1021 tcagttgctc tggcagattg acaaaaacac cctgacaaat acaggttgct tcggcagtca
     1081 cattgttatc agggatgata aaaataattc ttgctattgt tttattaaat tggacctctt
     1141 gagaacaatg gtcaaaagat aataaacgtc tccatgcaaa gaagaaaagc tccactgatt
     1201 ggtattctga tcatccttca gttcttgatt attgccggct gcagtcagaa aatggttttg
     1261 actgacagca acaggtcctc agggcttatt gttaactcat taattaatac taatttacct
     1321 gtattcatca atcggcagga aaacagtata attgttattt ctgtcgggtg tactgataat
     1381 ggacagaata agagagccag gaaggtggtt gttaattttt cacccggttc agatattgaa
     1441 gccgtaggtg aggtgtacgt taaatgccag gcttccggcc aggccggggc tgtcgtcttc
     1501 ggagcagcaa gagctgaaag aaaggtgaca attgatggag attatcaatt cagagcaggg
     1561 gaaaacctga tcactttggg aatatcccca tcgtctgttt cagaacttac tgacaaacta
     1621 gaggttgaaa gcgtaagtat tatacttgaa gatggttccg tgctgccagc gccgacggtt
     1681 tcatttcccc gggtcagatt tggaacagtt gtccgtgcag cagggcagga tggagcagat
     1741 acctaccgca ttccgggact agtgacaaca aatgaaggaa ccctgattgc tgtttatgac
     1801 atcaggtaca acaacagcaa ggaccttcag gaggatattg atatcggtat gagccggagt
     1861 actgatggcg ggcggacatg ggagccgatg aaagtgataa tggatatggg cgaatacgga
     1921 gggctgccac agaacctgaa cggtattggt gatccctgca tcctgtatga tcatactaca
     1981 ggcacactct gggtggcagc gctctggatg agcggctcgg cgcctgacaa gatgctctgg
     2041 agggcttcgc agccgggcat gacaccgcag gagaccggtc agttcatcct ggtaaagagt
     2101 actgatgacg ggcttacatg gtctgaaccg gtaaatatca cttcacagat caaggattct
     2161 gcatggcaac tgctcctcca gggaccggga agaggcatta cccttgatga cggcactctt
     2221 gtcttccctg cacagttcaa ggcagatatc ggaacgaagg ccctggacgg gggagagtac
     2281 acatgccact ctactattgt ctgcagtatg gacggagggg atacctggca gattggttcc
     2341 ggcgcaaaat caaatacaac tgaagcccag gtggtgcaac ttgctgacgg ctcacttatg
     2401 cttaacatgc gtgatgacag aaacaggagc gacaaaagtg aaacaaatgg cagggctgtg
     2461 gctgtcacca ctgacctggg aaagacatgg agagcgcatc cttcatccaa ttcggccctc
     2521 cctgaaccga actgcatggc cagcctcatc tcggcaaaga tcaatattgc cggtgacgac
     2581 agggatgttc tcttcttttc caatccgaat gacaggaaca acaggatcag gatgaccatc
     2641 aaggcaagca ctgaccaggg attgacctgg ccggaggaga accaggtgga gctgtttgct
     2701 cctgaaggtt atggatattc atgcctgaca atggttgatg aaacccacgt cgggatcatc
     2761 tatgaaggtg taaaggaact ctattttcaa aaaattcctg ttgccgatct gcttggtgac
     2821 agataaaaaa acagccccga acttatgaac ttccctgcaa aagaatggct ccttctgatc
     2881 ctgatcagcc tgtttacagg tgggatcatt tatccggttc agctgtcaga aaaaccatct
     2941 ctgatacctg cgcctaagat attcagtctg accgtagaaa agttcagggt cagacaggct
     3001 ggtttgtctg atgatcaaag aatcagaaag gttgttgtac cggctattga ggaagcccct
     3061 ctgaacatag aggaagcctt ccggattatt gtccgttcgg attcggttct gatctatgca
     3121 acgggagatg aaggattttt cagggctgac gcaacattga aacagcttgc aggaagtgac
     3181 actgacggtg aattcataca ggggtgttca atcactgact ggcctgcttt caggataaga
     3241 ggtttcatgc atgactgcgg caggagtttt attccggtca gtgagctgaa gagagaggtt
     3301 gagttactct cattattcaa gataaacacc tttcactggc acctgacaga aaaccttgca
     3361 tggcggcttg ccagcgatat catgccggaa ctgacagacc ctgatgtaac cctcagggat
     3421 aatcacggct attacaccag ggaggaggta agggattttg tcaggttctg cagggatcat
     3481 ttcgttgagg tcattccgga aatagacatg cctggccaca gtgccgcatt caccagggca
     3541 acaggccatt caatgcaatc agaggaggga aggaagatag ttgtgcaact gctcagggag
     3601 gcatgcaacc tctttgaagg cagctatttc catatcggca cggatgaagt caatattgcc
     3661 agtccggatt ttgtgcctga aatgatggat attgtcaggt cttacggaaa ggaggtaatt
     3721 ggctggcttc cgggagctcc catggatcag ggtgctctcc gccagttgtg ggctgatgtc
     3781 tccctgccgc aaaacacacc ggtgattgat tccaggtaca ggtacctcaa tcataccgac
     3841 tattacgctg atcttttttc aatttacaac tcaaggatgt gcgatgcgga gcagggctcc
     3901 gaccttctgg cgggaggaat ctgttgtata tggaacgaca ggaaaccggc atcggttgag
     3961 gatatactca tctccaatgc tttttacccc gtcatgctgg cttttgctga aagatcctgg
     4021 agcggaggag gagatgatat caagtcacgg ggagtcagga tgggactgcc cggagaccct
     4081 gccttcgaaa ggttcaggga gtttgaggca aggatgatct cattgaagga gagattcctc
     4141 aatgatgtgc catttcctta tctgcctcag acaggcattc tctggaatat cagcaggcag
     4201 tatccgaacg atggcaatcc ggactattcc gcccccttcg aaaatgatct ggtgacagat
     4261 cctgacaaag tatcgtctgc agatgcaggg ttcaggcctg ctgcaggggc cgcagtctat
     4321 ctcaggcata cctggggaga aaaggtgcca gccttctata ttgatcccgc tcctgaccat
     4381 acagcatatg cctgtacatg gatatacagc cccacagacc ggcaggcggg ccttcatttc
     4441 tcaacccaca actacggcag gtctgagctg gatatggctc ctcctcaggg tgagtgggat
     4501 tataaacgaa gccgggtatg gctcaacggg gaaccggttt cacctcctga atggaactac
     4561 actggtgtta aacctgacca tgaaactcca tataccaatg agaatatgtg gagccggcca
     4621 cctgcagcta tcactctcag ggagggctgg aacagcctgg tgataaaact gcctgtcgga
     4681 agtttctcaa ctccggagac aaggctggtg aagtggatgt tcacagccat gatagttaca
     4741 ccggatggtt ccgcgcaacc tgatggactt gtctggtcgc ctgataaact gtttcccggt
     4801 gataaaacca ggattgcctg cataggcaac tcggtaacct acgggacagg agtggcaggt
     4861 agggatacaa acgcttatcc tgctgttctt caacgtatgc tgggtggcag gtacatcgcg
     4921 ggcaacttcg gccggagtgg cgcaacgctg cttaagaagg gttacagacc atataaccgg
     4981 acggaggagt tcagggctgc cctttcattc aggcccgata ttgcagttat acaccttggc
     5041 ctcaatgaca ccgatccacg ggcatggcct gacttccgtg acgaattcat cagtgactac
     5101 ctctggctga ttgacacact caggtctgtg ggcacgaagg agttctatat tgccagactc
     5161 tctcccatat tccatggcca caggcggttc aaggccggaa cccgcgactg gtactggcag
     5221 attcaggaga tgattgagga ggttgcctcc ctctccggag ctaccctgat tgatttttac
     5281 accccgctca aagcgaggcc tgacctgatg cctgacaacc tccaccctga tgtccgtggt
     5341 gcagccatgc ttgctgagac tgtcttttcg gctttgacgg gcaattacgg aggccttcag
     5401 cttccgcagg cgtggagtga cggtatggtg cttcagcatg accgcgtcat caccctgaag
     5461 ggcaaagcaa acgggaagga acctgtagag gtctcccttg ccggcagatt atatcaggcc
     5521 atgacaacac ctacgggaga atgggaggta aaacttgatc cccttgaggc tggcggccca
     5581 tatcagctga tagcaatgac acgaagtgat actgttgtta tccgggacat tttggcaggc
     5641 gaggtctggt tctgctccgg ccagtcgaac atggaatgga ctgccggcaa ttccgacggg
     5701 tggcaggaga ttgccacctt acctccggat gaaaacctca ggctgataac attcagcagg
     5761 cgcgacttgt cgtggaacga gccatgggat tctgttacac ttgacaagat caataatctc
     5821 gactacttcc gtgagggtgc atggagggtt gcatcaccgg aaagtattaa ggatttttcc
     5881 ggtgtggcat attattttgg caggaagctc agggatgaac tcggggtgcc ggtgggatta
     5941 atagagatgg ctgtgggagg ttccccggcc gaggcatgga ttgaaagaaa ggctcttgag
     6001 atgcatcctc agctggttga cctgctctat gactaccgta aaagtgaatt cagggaacca
     6061 tgggtaagca atacggttac aaccaacctc aggaatagtg acaatccccg tcagcggcac
     6121 ccgtttgagc cggcttatct ctatgagtct ggtatttcac gcattgcagg atatccggtg
     6181 cgtggctttc tctggtacca gggagagtca aatgcaatat tctctgaact gtatgaagtg
     6241 atgttgccgg aactggcaag gaactggagg gatgcatggg atgatgatca cctgcccttt
     6301 tacttcgcgc agctctcgag cctcaacagg ccgtcatggc cacagttcag agatacccag
     6361 cgcaggctgg tatcagaaat accaaactcc ggcatggttg ttacaacaga tgccggacac
     6421 ccgacggatg ttcatccccg tgacaagaag agtgtgggag agagatttgc ctcccttgcg
     6481 ctcaatgata cctacggctt cagaaaggct ccccgcagcc cggagcctct ctccgctgaa
     6541 cgaaagggag acatggtaat catctcattc agctgttcag acagactgaa aacttcagac
     6601 ggattgcctc tcagggaaat cgaggtatca gaaagcggca attatttcag gcctgcgact
     6661 gcccgtattc gtggcaaccg actggaaatc aatgctccgg gagcaatagc agtaagatat
     6721 ggatggaagc catattcaac aggaaatctg accggtgaat caggattgcc ggtctcaaca
     6781 ttcagactta aaataaaatg acaatatgaa gattaagaaa cttgccggcc tgattgccgc
     6841 accgttcacc ccgatgtatc cggacgggga gatcaattcc gggataattg agcagtatca
     6901 cagagtgctt gtctccaatg gagtggcggg tgctttcata aacggctcat caggcgaggg
     6961 cgtctcaacc acgagccacg aaaagctgat tgttgcggaa gcctgggcaa aagcctcttc
     7021 aggtagcaac cttatggtta tcagccatct gggagggaca tgcctcaggg agtgcgttga
     7081 gctggccagg gagtcggaga ggatggggct ctatgcagtg tctgtgacat caccctcgta
     7141 cttcaagccg gcaaatgtga agatgctggc ggagtttttc atcagggtgg cagagtcagt
     7201 tcctgaaacg cctgtttatt actatcatat cccggtgctt accggtggct ttttcaacat
     7261 gattgacttt gtcagggagg tggctccgcg tgtgcccaac tttgccggaa tcaagtttac
     7321 gcacgaagac ttcatggatt tcctgagttg tctgaattat gacaatggca ggtatgatat
     7381 gctgtggggc agggacgaaa acttcctttc cgcactggtt cttggagccc ggggtgctgt
     7441 cgggagtaca tacaactatg ccgctcctct ctatcacaag ttgatagagg catttgacag
     7501 gaatgatcag gtcactgcac gcgcaatgca gcagaaatca attgacatga tcacccttct
     7561 cggcaaatac ggaggtattg ccacggggaa agcatatatg aggtttatag gccttgactg
     7621 cggtgagttc agactgccgg taaggaatat gtcagaggct gactaccagg catttgttaa
     7681 ggatgtcagg gaattgggaa tggatgatct gttttcaaaa atctgacaca ggtgaaacaa
     7741 caacggttgc aattcttcgg attcctgttg attctctctt tttcattaac aattaactcc
     7801 tgcagaacga tgaatgccga atggacggag cttgctcccc tgccgccttc ttctcccggt
     7861 gctatacaac caggtgtggc aggccccttt accggcctcc atggcaacac tttaatactt
     7921 gctggcggcg ccaactttcc tgataccatg ccatggcacg gcggcaggaa aaagtaccat
     7981 aatgagattt acctgc
//
LOCUS       DAOPBK010000123         7899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8385_length_7899_cov_5.043728, whole genome shotgun sequence.
ACCESSION   DAOPBK010000123 DAOPBK010000000
VERSION     DAOPBK010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8385_length_7899_cov_5.043728"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1198
                     /locus_tag="PLA17_07915"
     CDS             11..1198
                     /locus_tag="PLA17_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK85379.1"
                     /translation="MPSQRQLFLDHIGQTSPSPLMLEIERAEGSFLYDSNGRDILDLI
                     SGVSVSNTGHRHPAVMAALTGQAGRYMHLMVYGEVIQSPQVQYAGRLASLLPPPLESV
                     YFVNSGSEAIEGAIKLARRYTGRNEIIYFRNAYHGSTTGALSVQGSEEYRNAFRPLMP
                     ATTMAGFNTNEALEMISSGTAAVLVEPVQAEAGVVQPEEGFLDGLRSACSRHGAMLIF
                     DEVQTGFGRTGSLFSLSRYNVIPDILVLAKALGGGLPLGAFIASREIMSSLTRDPALG
                     HITTFGGHPLCCTTGLASLEVIVSEGLAGAARGKEKLFRKELAGMPFREIRGEGLLLA
                     VVPEKPEIIPAMISHAPEYGLLLDYFLFCSTAFRIAPPLTISEDEIRLACQRLKRLTE
                     AVS"
     gene            1366..3417
                     /gene="htpG"
                     /locus_tag="PLA17_07920"
     CDS             1366..3417
                     /gene="htpG"
                     /locus_tag="PLA17_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPK85380.1"
                     /translation="MEKGKIGVTTENIFPIIKKFLYSDHEIFLRELVSNATDAIQKLK
                     ALALAGEFKGELGELRVSVTLDPKKKTLTVSDNGIGMSAEEIDKYLNQIAFSSANEFL
                     DKYKDQASGLIGQFGLGFYSAFMVSDRVEVISKSYREDEPAVKWTCSGSPEYSIEEAQ
                     REERGTDVILHIDKDSKEFIDENRIEGLLKKYCNFLPVPVQFGNEKEWKDGKMVDTGK
                     PKIINNTRPAWTRKPTELKDEDYASFYRELYPMSEEPLFHIHLNVDYPFKLTGILYFP
                     KIRNNFEIQKNKIQLYCNQVFVTDSVEGIVPDFLTLLHGVIDSPDIPLNVSRSYLQSD
                     SNVKKISGHITKKVADRLTDIFKKDRETFEKKWDDLKLFIEYGMITEEKFFEKISKIA
                     LYKNTDGKYFTLEEYDKIIRENQTDKDKSLVHLYTTDPVAQHAYVAKAAEKGYDVLVL
                     DGQLDTHLINTLESKEPSSRFVRVDSDVIDRLIKKEESHTSKLTRSQSDDLVQIFRTQ
                     VSDTDKARFNVRAENLDENDLPMVITQSEFMRRMKDMAQFGGGYSFYGEMPDSFDLVL
                     NTNHPLVISLAGDLDKEHADELKKNGAGIEKTKVEIEFMEKEHAKKKPEELLSVEKDD
                     LERLRGELKQLEEKRKTILGGFGNDHQAVRQLIDLAMLANNMLKGEALSAFVKRSISL
                     L"
     gene            complement(3549..4073)
                     /locus_tag="PLA17_07925"
     CDS             complement(3549..4073)
                     /locus_tag="PLA17_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85381.1"
                     /translation="METFLEILKISIPALLVMLTAWLVIRNMLRDDQERRRQELLLQT
                     VKTITPVRLQAYERVVLFLERISPESLIMRTARPEMTSLQLQSALVNAVRSEYEHNLS
                     QQIYMSSEAWEMVKNARGTIVRMINSVASQLPPNATGEELSRLLLEEVVEMDAEPTRT
                     AIAYIKSELARIMG"
     gene            4319..5512
                     /locus_tag="PLA17_07930"
     CDS             4319..5512
                     /locus_tag="PLA17_07930"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPK85382.1"
                     /translation="MEHISRRVQALSVSQTLAMAQKSRELKAQGIDVINLSLGEPDFN
                     TPDDIKEAAKKAIDDNYSFYPPVPGYNDLREAISRKFKEENGLDYTPDQIIVSAGGKH
                     SLINVMLSVVDPGEEVILLAPYWVSYLDHITFAEGKPVIIQTSIDSDFKVTPAQLEAA
                     LTPRTRVLIFNSPSNPTGMVYTREEMGALVKVLEKYPQVIIISDEIYEHITFSGKHVS
                     MASFSSICDRVVTVNGVSKGYAMTGWRIGYIGGPLWLAKACNKLQGQFTSGVSSISQR
                     AALAAITSKSGSKERMREAFLRRRDLICRLLGEIPGLKVRVPQGAFYVMPDISNFIGK
                     SFNGRVMKNANDLTFYLLEEASVAMVSGTAFGADNCIRISYATSDDRIIEAVRRIKEA
                     LAKLG"
     gene            complement(5652..6203)
                     /locus_tag="PLA17_07935"
     CDS             complement(5652..6203)
                     /locus_tag="PLA17_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPK85383.1"
                     /translation="MIDGEKMLSLMKGRQSERRFLSKPVESEKIERITAAGRLSPSAC
                     NGQPWHFIVVDEPALRDEVAAATETAVLRMNAFVREAPVLIVVVREKSNLASRAGDLI
                     KQKDYSLIDIGIATASMVYQAAAEGLGTCIIGWVDDKRIRQSFGIPASRKVELVIAVG
                     YTDNRLREKSRKPPEDIITYNRY"
     gene            complement(6220..6795)
                     /locus_tag="PLA17_07940"
     CDS             complement(6220..6795)
                     /locus_tag="PLA17_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPK85384.1"
                     /translation="MKGIKGTRTEQNLLKAFAGESQAKNRYEFAAKVAKKEGYEQIAA
                     IFLETALQEQQHAKRFFNYLEGGMVEITAAYPAGQTLTTKDNLEAAAEGEHEEWAELY
                     PMFAKVAEEEGFKEIAACFKAISAAEKTHEDRYRKLLENVMGDKVFERDEKVFWYCRK
                     CGYVHFGTKPLKNCPACLHPFNYTEVYAQNY"
     gene            6899..7807
                     /locus_tag="PLA17_07945"
     CDS             6899..7807
                     /locus_tag="PLA17_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPK85385.1"
                     /translation="MKKTLFVLLAVILAALQSCNPKGTEYHLYYLGGQSNMDGYGYVS
                     QLPSELTGEYEGIMIYHGNPVGDNTTIDGRGLWTTLCPGHGAGFASDGTANTYSDRFG
                     VELTFAGRMKELFPDEKIAIVKYSRGGTSIDTAAAGGAGAWLPTYTGGNGINQYDHFL
                     ATVEGALSVGDIDGDGKTDRLIPSGILWMQGESDANNDYATSVYKENLATLIDSIRKA
                     FSTPGMPVAIGRISDSHNDTDSLVWTFGDAVREAQARFVAEDGNAALVTSTDNYGYSD
                     KWHYDSEGYIDLGREFAEAIAGLRRR"
BASE COUNT         1982 a         2033 c         2058 g         1826 t
ORIGIN      
        1 tgcagcgctg atgccttcac aaaggcaact tttccttgac catatcggac agacctctcc
       61 ctcgccactc atgcttgaga ttgagagggc ggaaggttca tttctgtatg acagcaacgg
      121 ccgtgatatc cttgatctta tctcaggtgt atcggtcagc aacaccggcc atcgccatcc
      181 ggcagtcatg gctgcactga ccggacaggc cggaaggtat atgcacctca tggtctacgg
      241 cgaggtgata cagagtccac aggtgcagta tgccggacgc cttgcgtcac tgctgcctcc
      301 cccgctggag tcagtctatt ttgtcaactc gggaagcgaa gccatagagg gagccatcaa
      361 gctggcacgc aggtataccg gtcgcaatga gatcatctat ttccgcaatg cttaccacgg
      421 atccaccacc ggggctctca gcgtccaggg ctcggaggag tacagaaacg ccttcaggcc
      481 tctgatgcca gccacaacca tggccgggtt caacaccaat gaggctctgg agatgatcag
      541 cagcggtact gccgccgtcc tcgttgagcc cgtgcaggcc gaagcgggtg tggtgcagcc
      601 ggaagagggc ttcctggacg gacttcggag tgcatgcagc aggcacggcg ccatgctgat
      661 tttcgatgag gtgcagacag gattcggccg gaccggcagc ctcttttcac tcagcaggta
      721 caatgtcatt cctgacatcc tggtgctggc aaaagcgctc ggcggaggac tgccacttgg
      781 agccttcatc gcttcacgtg aaataatgtc atccttgacc cgtgacccgg cactgggaca
      841 tataaccact tttggaggac atcctctctg ctgtacaaca gggttggcat cactggaagt
      901 gattgtcagt gaaggccttg ctggcgctgc acggggaaag gaaaagctgt tcagaaaaga
      961 actggcagga atgcctttca gagagataag gggcgagggt ctcctcctgg cagtggtacc
     1021 tgaaaaaccg gaaatcatcc cggccatgat ttcccacgca ccggaatacg gactgctgct
     1081 cgattatttt ctcttctgtt ccacagcctt tcgcatagcg cccccgctca ctatctccga
     1141 agatgagatc cgcctcgcat gccagaggct gaaaaggctg acagaagcgg tgagctgacc
     1201 aggttttaca attcctgatg ccttcctgag gttcttcaac gcatccggtt atctgattat
     1261 gatgctgact ggtgattggt gactccggac tgccactctg tcagacttac tgccgcggca
     1321 cgtcctttga agattggggg gcagaataat cagaaaacca aaaatatgga aaagggtaaa
     1381 atcggagtaa ccacagaaaa catctttcca atcatcaaga aatttctcta ttcagatcat
     1441 gagatcttcc tgagagaact ggtatcaaac gctacagacg ccatccagaa gctgaaggcg
     1501 ctggccctgg caggagaatt caagggcgag ctcggtgaac tgcgagtaag cgtcactctt
     1561 gatcctaaaa agaaaacact taccgtctct gacaatggca tcgggatgag cgctgaagag
     1621 atagacaaat atctgaacca gatagccttc tcgagtgcta acgaatttct cgacaaatat
     1681 aaggatcagg ccagtgggct cattggccag ttcggtctcg gattctattc ggcattcatg
     1741 gtctcagaca gggtcgaagt catttcaaag tcatatcgtg aggatgagcc tgcagtgaag
     1801 tggacctgca gcggcagccc ggaatactct attgaagagg cgcagaggga agagcgcgga
     1861 acggacgtca tccttcacat cgacaaggat tcaaaggagt tcattgatga gaaccgcatc
     1921 gagggcctcc tgaagaaata ttgcaacttc ctgccggtgc cggtgcagtt tggcaatgag
     1981 aaggagtgga aggacggcaa aatggttgat accggcaagc cgaagatcat taacaatacc
     2041 cggccggcct ggacacgcaa acccactgaa ctgaaggacg aggattatgc aagcttctac
     2101 cgcgagcttt atccgatgag cgaggagcct ctctttcata tccacctgaa cgttgattat
     2161 ccgttcaaac tgaccgggat cctctacttc ccgaagatca gaaacaactt cgagatccag
     2221 aaaaacaaga tacagctata ctgcaaccag gtattcgtga ctgactccgt ggaaggtatt
     2281 gtccctgact tcctgacgct gttgcacggt gtgatagact ccccggatat cccgctgaac
     2341 gtctccagga gctacctcca gagtgattcc aacgtcaaga agataagcgg gcatatcacg
     2401 aagaaagtgg cagaccgcct gacagatatc ttcaagaagg accgcgagac tttcgaaaag
     2461 aaatgggatg acctgaagct tttcattgag tacggcatga tcaccgagga gaaattcttt
     2521 gaaaagatat caaagatcgc cctgtataaa aacacagatg gcaaatactt cacccttgag
     2581 gagtatgaca agatcatcag ggagaaccag accgataagg acaagtcgct ggtgcacctc
     2641 tatacaactg acccggttgc ccagcatgcc tatgtggcca aagctgcgga aaagggttat
     2701 gacgttctgg tacttgacgg acagcttgat acacatctca taaacaccct tgagtcaaag
     2761 gaaccttcat caaggtttgt aagggttgac agtgacgtga ttgacaggct tatcaagaaa
     2821 gaggagagcc atacctcgaa gcttacccgg tcgcagtcgg atgatctggt gcagattttc
     2881 cgcacacagg tgtctgacac tgacaaggca cgtttcaacg taagggctga gaacctggat
     2941 gagaatgacc ttccgatggt catcacgcag agcgaattca tgcgcaggat gaaggatatg
     3001 gcgcagtttg gcgggggata ttcgttttac ggagagatgc ctgacagctt cgaccttgtg
     3061 ctgaacacca accacccgct ggtcatttca ctggcaggag accttgataa ggaacatgcc
     3121 gacgagttga agaagaacgg tgcgggaatc gaaaaaacga aggtcgagat tgagttcatg
     3181 gagaaggagc acgccaagaa gaaaccagaa gagcttcttt cagttgagaa ggatgatctt
     3241 gaaaggctca ggggcgagct gaagcagctt gaggagaaga ggaagaccat tctcggcggg
     3301 ttcggaaatg accatcaggc tgtgaggcag ctgatagacc tcgccatgct ggcaaacaac
     3361 atgctcaagg gagaggcgct cagcgcattc gtcaagcgaa gcatcagcct cctgtagaac
     3421 tctttttctt tccgtatctg aacgcactgc gttcagaata gtccgggacg gcatccgtcc
     3481 ggctatccca ttatccgggc cagcatctgt tcggcttatc ctattatccg ggtcagcatc
     3541 cgtccgggct atcccattat ccgggccagc tctgacttga tgtatgctat tgctgttctg
     3601 gtgggctcgg catccatttc tacaacctcc tcgaggagca accttgagag ctcttcgccg
     3661 gtggcgttgg gtggaagctg ggaggcaacg ctgttgatca tcctgacaat ggtgcccctg
     3721 gcattcttca ccatctccca ggcctcacta ctcatgtata tctgctgcga tagattgtgt
     3781 tcatactcgc tcctgaccgc gtttacaagc gccgactgaa gctgcaggga ggtcatttcc
     3841 ggtctcgcgg tgcgcattat aagcgactcc ggggagatgc gctccaggaa cagtaccacc
     3901 ctctcatagg cctgaagcct gacaggcgtt atggtcttta ccgtctgaag cagcagctct
     3961 tgcctccggc gctcctggtc gtcgcgcagc atgttgcgta taaccagcca cgcggtgagc
     4021 atcaccagca gggcaggtat ggatattttg agtatttcaa ggaaggtttc cataatagca
     4081 gttgtttaaa gtacatgtga gatgcaaatt aatcaatttt cagcctttcc tgacaggtta
     4141 ataaagattt ggcaggatta ttcagatatc atcctgtcag gatggacccg gtttacacgg
     4201 gttcaattac attgattccc gccatgccac cccggcctgc cataatccct tttctttttc
     4261 atttttaaca attacatttg acatttttcg gtaacgaacc caataaaaaa ctgacacaat
     4321 ggaacatatc agcaggaggg tacaggcact ctcggtatca cagacattgg cgatggcaca
     4381 gaaaagccgc gaactcaagg cacagggtat tgacgtaatc aacctgagtc tgggcgaacc
     4441 tgacttcaat acccctgacg atatcaagga agccgcaaag aaagccatag acgacaacta
     4501 ctcgttctat cctcccgtgc ccggttacaa tgacctgcgc gaggctatat cacgcaagtt
     4561 caaggaggag aacgggcttg actacactcc cgatcagatc atagtgtcag caggagggaa
     4621 gcacagcctc atcaatgtta tgctttcggt ggttgacccg ggcgaggagg tgatactgct
     4681 tgctccatac tgggtcagct atcttgatca tatcaccttc gccgaaggca agcctgtgat
     4741 aatccagaca tcaatcgata gcgatttcaa ggtgacgccg gcacagctcg aggcagctct
     4801 cactccgcgc accagggtgc tcatcttcaa ctcgccctca aaccccacgg gaatggtcta
     4861 cacgcgggag gagatgggag cgctggtgaa ggttcttgag aagtacccgc aggtgatcat
     4921 catctcggac gagatctatg aacatatcac cttctccggg aagcacgtca gcatggcatc
     4981 gttcagcagc atctgcgaca gggtggtgac tgtcaacggc gtctcaaaag gatatgccat
     5041 gacaggctgg aggataggct atattggcgg accgttgtgg ctggcaaagg cctgcaacaa
     5101 gctgcaggga cagttcacat caggggtctc ctctatttca cagagagcag cgctggcagc
     5161 catcacctcg aaaagtggct cgaaggagag gatgcgcgag gcattcctcc gccgcaggga
     5221 tctgatatgc aggcttctgg gcgagatccc cggtctcaag gtaagggtac cccagggcgc
     5281 attttatgtc atgcctgaca tcagcaactt tataggcaag agctttaacg gcagggtgat
     5341 gaagaatgcc aatgacctga ccttctacct ccttgaggag gccagtgtgg cgatggtcag
     5401 cggaacagcc ttcggcgccg ataactgcat caggatctca tacgccacct ctgatgaccg
     5461 tattatagag gcagtccgcc ggataaagga ggcgctggcg aaactgggat gatcattccc
     5521 tgaatggaga tattctgcat ggatgcggat ggcgccaggt ctcagacctg gcgttacctg
     5581 cctgtgatgc agatatcgtg tatattgaat gcagcgccgg gtctgagacc cggcgttacc
     5641 tgtatgtaat atcaatatcg attgtaggtg atgatatctt ccggtggttt gcgcgatttt
     5701 tccctcagac ggttgtcagt ataacccacg gcgatcacca gttcaacctt tcttgatgcc
     5761 ggaatgccaa aggattgcct gatccgttta tcatctaccc accctatgat gcatgtacca
     5821 agcccctccg cggcagcctg gtaaaccatc gatgccgtag ctatcccgat atcaatcagt
     5881 gaataatcct tttgcttgat caggtctcct gcacgggagg cgagattgga tttttcacga
     5941 accaccacta tcagtacagg cgcctccctg acgaaggcgt tcatccgcag cacagccgtt
     6001 tcggtggcgg ctgccacctc atcacgcaga gccggctcgt caaccacaat gaaatgccat
     6061 ggctggccgt tgcaggcaga gggtgacaac cttcccgccg cagtgatcct ttcaatcttc
     6121 tcgctctcaa ccggtttgct gaggaatctc ctttcactct gcctgccctt catcaggctg
     6181 agcatctttt ctccgtcaat cataccgcca ggcaggagat caatagttct gcgcgtatac
     6241 ctcggtgtaa ttgaacgggt ggagacatgc cggacagttc ttcagcggct tcgtgccgaa
     6301 gtgcacataa ccgcacttgc ggcagtacca gaagaccttc tcgtcacgct cgaacacctt
     6361 gtcgcccata acgttctcaa gcaacttcct gtaacgatcc tcatgcgttt tctcggcagc
     6421 agagatggcc ttgaagcagg cagcgatctc tttaaatccc tcctcctcgg ccactttggc
     6481 aaacatcgga tagagttcag cccactcctc atgctcaccc tcagcagcag cctcaaggtt
     6541 atctttggtg gttaaggtct ggccagcggg ataggcagcg gtgatctcaa ccatgcctcc
     6601 ctcaagataa ttgaagaaac gcttggcatg ctgctgctcc tgcagcgctg tctcaaggaa
     6661 gatggctgct atctgctcat agccctcttt ctttgccacc ttggcagcaa attcataacg
     6721 gtttttagcc tgtgattcac ctgcaaatgc cttcaggaga ttctgttccg tacgggttcc
     6781 ttttattcct ttcatttttt ctttttttga aattgattaa gcttgaaaga gcacaaaaat
     6841 agtgaaattt tggctacttt taggcaactt aaccgcaatt aaaacattcc ttttaactat
     6901 gaaaaaaaca ctatttgtcc tgcttgctgt aattcttgca gcgcttcagt cgtgtaaccc
     6961 gaaagggact gaatatcact tatattacct tggtggccag tcaaatatgg atggctacgg
     7021 ttatgtatca cagctccctt ccgaactcac tggcgagtat gaaggcatca tgatttatca
     7081 cggtaacccg gtgggagata ataccacgat tgacggtcgc ggactatgga ccaccctctg
     7141 ccctggacac ggcgccggct tcgcaagtga cggcactgcc aacacctatt cagatcgttt
     7201 tggcgtggaa cttacctttg ccgggagaat gaaggaactc ttccctgatg agaaaattgc
     7261 gatagtcaag tattccaggg gaggaacttc aattgacact gcagcagcgg gtggcgccgg
     7321 agcatggctg ccgacatata ccggaggcaa cgggatcaac cagtatgacc acttcctcgc
     7381 cactgtagaa ggagcgttga gtgttgggga tattgacggc gacgggaaaa cagaccggct
     7441 gattccctca ggaattctct ggatgcaggg tgagagtgac gcaaataatg attatgccac
     7501 atctgtctat aaggagaacc tggccaccct cattgacagc atccggaaag ctttctctac
     7561 tcccggcatg ccggtggcaa ttggccgtat atctgattcg cacaacgata ccgacagcct
     7621 tgtctggaca tttggtgatg ccgtcaggga agcccaggca aggtttgttg cagaagatgg
     7681 caacgccgca ctggtaacct caactgacaa ttacggttac agtgacaagt ggcattatga
     7741 cagtgaaggg tacattgacc tgggcaggga gttcgccgaa gcaattgccg gactgagacg
     7801 tcgttaggcc ggaataattc ataaataatt gcaggattga gacgtcgcca ggccggaaca
     7861 tttctgaagc tgttgcctga ctgaggcatc gccaggccg
//
LOCUS       DAOPBK010000124         7876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8409_length_7876_cov_4.987981, whole genome shotgun sequence.
ACCESSION   DAOPBK010000124 DAOPBK010000000
VERSION     DAOPBK010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8409_length_7876_cov_4.987981"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PLA17_07950"
     CDS             <1..1210
                     /locus_tag="PLA17_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPK85386.1"
                     /translation="HNNIKGSYADGRYDTVAIPGTNLWSTIDLALQEYGERLMQNKTG
                     SIVAIEPSTGEILTLVSSPSYDPSLLVGRVRSDNFAALQADTVKPLFDRALMASYPPG
                     STFKIMNGIIGLQENVIRPTSLFSCNYGYHAGSLTVGCHSHASPLNLPQAVAQSCNAW
                     FCNAFRSILENPSYGSIQDAFDKWREYLVAFGFGNKTGIDLPNELPGNIPTRSYYDRY
                     YGKDRWRALTVISLAIGQGEIGATPLQMANMAAVIANRGSFYIPHVIKKIGDDGTPSP
                     LYTQKYITGISPENFQPVIEGMEGAVNGGPGSTARGARLDSIIICGKTGTAQNPHGKD
                     HSIFVAFAPKDDPEIAIAVYVENAGFGSTFAAPIASLMIEKYLTGNIKRKYLETHVLN
                     MVITPSGKEQ"
     gene            1194..2609
                     /gene="rodA"
                     /locus_tag="PLA17_07955"
     CDS             1194..2609
                     /gene="rodA"
                     /locus_tag="PLA17_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPK85387.1"
                     /translation="MERSNNILARLDWVTVMLWLVMMIMGWLNIYAAVYNEQHTSIFD
                     MSQRYGKQLIFIIAAIVLAVMVMVTDNKFWFFFAWFIYGFFILMLILVLIIGKEINGA
                     KAWFGVGAFSIQPSEFAKFATGLALASYLNSKRQMLGAREVLAATAIIIVPVLLIALQ
                     PDMGSVVTYFAFFIVLYREGVSIWVFITAILAVALFFLTLILGNLPVAIGLLVAAAIV
                     LLITSGYMLTLKAAAVMAVTGGVAFLIGHYILDLDTVLIIVLAVILAGMVYAWFIYRL
                     KLVNQAIIYAFLLGGLLYLFTVDYAFHTALKPHQQTRINIMLGMEDDPFGEGYNLNQS
                     LISIGSGGFTGKGYLNGTQTKFKFVPEQSTDFIFCTVGEEWGFIGSLVVIGLFVWLLL
                     RLMMMAERQRTTFVRAYGYGVFALFLVHFFVNIGMTIGVMPVIGIPLPFFSYGGSSLW
                     GFTMLLFIFLRLDAGRTEYLG"
     gene            complement(2620..3231)
                     /locus_tag="PLA17_07960"
     CDS             complement(2620..3231)
                     /locus_tag="PLA17_07960"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HPK85388.1"
                     /translation="MNVLAHIYLSGDSDNIIIGNYIGDYVKGHDYLKYPEGIRKGIII
                     HRHIDAFTDRHPVVHRSKLHFTGRYRKYAGVVIDILYDHYLTKEWDLFSRRPLESVTY
                     NFYRAMVNNYEILPESVREKMPFFIIDNWLETYQTRRGIRRVLKTLSSFTSLPKETRW
                     AMRVWNKQYYSIEEDFMEFFPQLISYVESEFGIDLSPEISMPF"
     gene            3479..4819
                     /locus_tag="PLA17_07965"
     CDS             3479..4819
                     /locus_tag="PLA17_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPK85389.1"
                     /translation="MLKRNVIIIGAAGRDFHNFNVFFRDNELYNVVAFTAAQIPDIDG
                     RKYPAALAGKLYPDGIPIYSENDLPRLISELRVTDCVFAYSDVPYQRVMALSAIINAA
                     GANFMLLGWKETMLTSTKPVIAVGAVRTGGGKSETSRRIVDELKRHGLKVIAIRHPMP
                     YGDLVKQAVQRFETIDDLHKHECTIEEMEEYEPHVVRDTIIYSGNDYEPILRKAESDP
                     AGCDVILWDGGNNDFPFIKPDLMITLVDPQRAGHELTYYPGEITLRLADVVIVNKIES
                     ADLNDLFEVRKNVAKLNPDAVIVDACSPTVVQDPGLIRGKRVLVIEEGPTVTHGEVKY
                     SYGCFTAMKYGAAELVDPKPYAVGKLKDTFDDYPNLGPVLPSMGYSNQQMKDLSETIK
                     NTPCDLVLIATPIDLKRVIEIDKPTLHVSYDLQEIGHPDLGDVIDEFVKKHKLA"
     gene            4965..6305
                     /locus_tag="PLA17_07970"
     CDS             4965..6305
                     /locus_tag="PLA17_07970"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPK85390.1"
                     /translation="MKQTISINIKGISSFADSSALNSIRARAIANLKTLREGTGRGNE
                     FTGWLRLPDRAVNETTAVRACAARLRAQAPVTVVVGIGGSYLGSRALIEALNDPFAPK
                     EHTLLFAGHTLSEDYHAALLRFLDGTEYNIVVISKSGTTTEPAIAFRVLRDHMRNKLG
                     TAAMKSYIVAITDARRGALHDLAVSEGYETFVIPDDVGGRYSVLTPAGMLPLALAGYD
                     IDAFARGMLDMALSVREGNDNPENIAVTYAAARNALYGRGYSTEILVSYEPSLVFLAE
                     WWKQLYGESEGKEGKGIFPASVSFTTDLHSMGQYIQQGERKLFETVIHVADSYHRLVV
                     PGATDNSDGIGFIAGKRMTEVNHKAEDGTRIAHIDGGVPVIVVSMERIDEYNLGQLMY
                     FFELACAISGYILGVNPFDQPGVESYKKNMFALLGKPGFESERARLEQDLRDKE"
     gene            complement(6252..7862)
                     /gene="lysS"
                     /locus_tag="PLA17_07975"
                     /pseudo
     CDS             complement(6252..7862)
                     /gene="lysS"
                     /locus_tag="PLA17_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458069.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
BASE COUNT         1917 a         1915 c         2027 g         2017 t
ORIGIN      
        1 gcacaacaac atcaaggggt catatgccga tggaaggtat gacacggtgg ccatcccggg
       61 caccaacctg tggtctacca ttgaccttgc cctccaggag tacggtgaac ggctgatgca
      121 gaacaagaca ggctcaatag ttgcaatcga accctcaaca ggcgaaattc tcactctcgt
      181 ttcttctccc tcctatgatc cttcactgct ggttggaaga gtcaggtcag ataattttgc
      241 tgccctgcag gctgatacgg taaagccgct ctttgacagg gccctgatgg cctcatatcc
      301 gcccggttcc accttcaaga taatgaatgg catcataggc ttgcaggaga atgttatcag
      361 gccgacttcc cttttcagtt gcaactacgg ttaccatgcc ggatcgctga cggtaggctg
      421 tcactcgcat gcctcgcccc ttaaccttcc ccaggctgtg gcacagtcgt gcaacgcctg
      481 gttctgcaac gccttccgca gtatactgga aaacccctca tatggatcaa tacaggatgc
      541 atttgacaag tggcgggagt acctggtggc tttcggcttc gggaacaaaa cagggataga
      601 cctgccaaac gagttgccgg gaaacattcc cacgaggtca tactatgatc gttattacgg
      661 taaggaccgc tggagggctc tcacggtgat ctcactggct atcggtcagg gggagattgg
      721 cgccacaccg ctgcagatgg ccaatatggc agccgtaatt gccaacaggg gttcatttta
      781 tattcctcat gtgatcaaga agataggtga tgacggaaca ccctcacccc tctataccca
      841 aaaatatatt acaggtatct caccggagaa ttttcagcct gtaattgaag gaatggaagg
      901 agccgtcaac ggaggccccg gttctacggc acgcggtgca cgccttgaca gcatcattat
      961 atgcggaaag accggcacgg cacagaaccc gcacgggaaa gaccactcca tcttcgtggc
     1021 ctttgccccg aaggatgacc ctgagattgc aattgcagtt tacgttgaga atgcaggttt
     1081 tggctccaca tttgcagccc cgattgccag cctgatgata gaaaaatacc ttacaggcaa
     1141 cattaaaaga aaatacctgg agacccatgt cctgaatatg gtaataacac caagtggaaa
     1201 ggagcaataa catactggca aggcttgact gggtcacggt gatgctgtgg ctggtaatga
     1261 tgatcatggg atggctgaat atttatgcgg ctgtctacaa tgaacagcat accagcatct
     1321 ttgacatgtc acagcgctac gggaaacagc tgattttcat tatcgcggca attgtcctgg
     1381 ctgttatggt gatggttact gacaataaat tctggttctt ttttgcatgg ttcatctacg
     1441 gtttctttat cctgatgctc atactggtgc tgattatcgg gaaggagatc aacggtgcca
     1501 aagcctggtt cggagttggc gccttcagca ttcagccctc tgagtttgcc aagtttgcca
     1561 ccggcctggc gcttgcatcg tatcttaact ccaaacggca gatgcttggc gccagggagg
     1621 tattggccgc aacagctatc atcattgtcc ctgtactgct gattgccctc cagcctgaca
     1681 tgggatcagt ggtaacatat tttgcatttt tcattgtcct ctaccgcgag ggggtaagca
     1741 tatgggtatt tatcaccgca atacttgccg tggcattgtt cttcctgacc ctgatactcg
     1801 gcaacctgcc ggtggccata gggctgctgg tggcagcggc tatagtcctc ctgattactt
     1861 ccggatacat gctgaccctt aaggcagcag cagttatggc tgtaaccgga ggagtggctt
     1921 tccttatcgg gcattatatt cttgatcttg acacggtgct tatcattgtt cttgcagtga
     1981 tccttgcagg aatggtgtac gcctggttca tttaccggct gaagctggta aaccaggcaa
     2041 tcatatacgc tttcctgctg ggtggattgc tctacctttt taccgtagac tatgcatttc
     2101 atacggcgct gaagccccac cagcagacca ggatcaatat catgctgggc atggaagatg
     2161 accccttcgg ggaaggatac aaccttaacc agtcactcat ctcaatcggc tccggcggat
     2221 tcaccgggaa aggctatctc aacggtaccc agacgaagtt caagttcgtt cctgaacaga
     2281 gcactgactt catcttctgt actgtaggag aagagtgggg gtttatcggt tccttggttg
     2341 tgatagggct gtttgtctgg ctgcttctca ggctgatgat gatggctgag cgacagagga
     2401 caacctttgt gcgcgcttat ggttatggag tattcgcgct tttcctggtc cacttctttg
     2461 tcaatatcgg gatgaccatc ggggtaatgc ccgtgatagg catacccctt ccattcttca
     2521 gttacggggg gagttcgctc tggggattca ccatgcttct gttcatcttc ctgaggcttg
     2581 acgcaggacg aaccgagtac ctgggctgat cagggctgat cagaagggca tgctgatttc
     2641 cggcgaaaga tctatcccga actctgattc aacatagctg atcagttgcg gaaaaaactc
     2701 catgaaatct tcctcaatgg agtaatactg tttattccag accctcattg cccagcgcgt
     2761 ctcctttggc agcgaggtaa agctgctgag cgttttcagc accctgcgta ttcccctgcg
     2821 ggtctggtat gtctcaagcc agttgtcaat aatgaagaat ggcatctttt cacgtactga
     2881 ctcgggcagg atctcataat tgtttaccat ggccctgtag aagttatagg ttacgctctc
     2941 aagaggtctc ctggaaaaga ggtcccattc ctttgtaaga taatgatcat aaagaatgtc
     3001 aataaccaca ccagcatact tgcggtaacg ccctgtgaag tgcagcttgc tccggtgaac
     3061 caccggatgc ctgtcagtaa aggcatcgat atggcggtga atgatgattc ccttgcgtat
     3121 tccctcaggg tacttcaggt aatcatggcc cttcacatag tcgcctatgt aattgccgat
     3181 gattatgtta tcagagtcgc ctgagaggta tatgtgcgcc aggacattca tccgccaaaa
     3241 gttagtgcaa agattctgcc aatatgtttt ggtttaaaat attaagaagc gaattaatca
     3301 acaaacccga ctgacttatt aacttgcata atatcaaaat attataagtc tcagaaagat
     3361 tggatgaaac tgattattat cacttccgat ttcagttaca aattataaat ttgcgggata
     3421 ctccaattga cttatacaat tattatttaa aaaaatcaat aactaactag gaggttatat
     3481 gttaaaaagg aatgttatta ttattggtgc cgcgggcagg gattttcaca atttcaatgt
     3541 ctttttccgt gacaatgagc tttacaatgt ggtagccttt acagctgccc agatacccga
     3601 tattgatggc aggaaatatc cggctgcact ggcgggaaaa ctctatcctg acggtattcc
     3661 catctattct gaaaatgacc ttcctcgttt gatcagtgaa ctcagggtta ctgactgtgt
     3721 cttcgcatac agtgatgtcc cctatcagag ggtaatggct ctgagtgcca ttattaacgc
     3781 tgccggagcc aacttcatgc tgcttggctg gaaagaaacc atgttgacaa gcaccaaacc
     3841 ggtgattgca gtcggtgcag tcagaaccgg gggtggaaag agcgaaacat cacgcagaat
     3901 agtggatgaa ctgaaaagac atggtctgaa ggtaatagcc atcaggcatc cgatgcctta
     3961 tggtgatctg gtgaaacagg cagtgcagcg ttttgagacg atagatgatc tccataagca
     4021 tgaatgcacc attgaggaga tggaggagta tgaaccacat gttgtccgtg ataccataat
     4081 ctactccggc aatgactatg aacccattct gaggaaggcg gaaagtgatc cggcaggatg
     4141 tgatgtaatc ttatgggatg gtggaaataa tgattttcca ttcattaagc ctgacctgat
     4201 gataacactt gttgatccgc agagggcagg ccatgagctg acttattatc cgggtgagat
     4261 aaccctcagg cttgctgatg tggttattgt gaataaaata gaaagcgctg atctgaatga
     4321 cctgtttgag gtaagaaaaa acgtggcaaa attgaatccg gatgctgtta tcgttgatgc
     4381 atgttcaccc acagtggtgc aggaccccgg gttaatacgc ggtaaaaggg tactggtcat
     4441 agaggaagga cctacagtga cacacggaga ggtcaaatac agttatggtt gttttactgc
     4501 aatgaaatat ggtgcagccg aactggtaga tcccaagccc tatgccgtcg gtaaactgaa
     4561 ggacaccttt gatgactacc cgaatctggg cccggtcttg ccctcaatgg gttacagtaa
     4621 ccagcagatg aaagacctca gtgagacaat caagaataca ccctgtgacc tggttttgat
     4681 tgccacgccg atagacctga aaagggtgat agaaattgac aaaccgactc ttcatgtcag
     4741 ttatgacctc caggagatag gccaccctga tcttggtgat gtaattgacg agtttgtaaa
     4801 gaagcacaag ctggcctgac aaggcaacag agagttgagg gatcaccgca gactattcag
     4861 cattgttgga tgtatgcgat aatgacacta ttccacacag gtgattactg attgtaaccc
     4921 tgccatggaa tcaatatctt tgtcttgacc taaaacataa ggcgatgaaa cagacgatct
     4981 ccatcaacat aaagggaatc agttcttttg ctgatagttc tgcgctgaat tcgatccgtg
     5041 ccagagccat tgcaaacctt aagacgctgc gtgagggtac cggccgcggc aatgagttta
     5101 ccggctggct gcgtctgcct gacagggctg tcaatgagac tacggcagtc agagcatgtg
     5161 cagcgcggct ccgtgcccag gctcccgtga cagtggttgt cggcataggt ggctcatacc
     5221 ttggttccag ggctctcatc gaggctctga atgacccctt cgctccgaag gagcatacgc
     5281 ttctctttgc aggccatacc ctcagcgaag actaccatgc agccctgctc agattccttg
     5341 acggcactga atataatata gttgtcatct ccaagagcgg cactaccacc gagccggcca
     5401 tagctttccg ggtactgcgt gaccacatga ggaacaagct gggcacggct gccatgaagt
     5461 catatattgt tgccatcacc gatgcccggc gtggagcatt gcatgatctg gccgtgagcg
     5521 agggttatga gacctttgtt attcctgatg acgtaggcgg ccgctattcg gtgctcaccc
     5581 cggcaggtat gctgcccctg gcgctggcag ggtatgacat tgatgctttt gcacgtggca
     5641 tgcttgacat ggccctctct gtcagggagg ggaatgacaa ccctgaaaat attgccgtca
     5701 cctatgcggc agcccgtaac gcactgtatg gcagggggta ctcaaccgag atacttgtca
     5761 gttatgagcc ttcgcttgtt ttcctggccg aatggtggaa gcaactctac ggcgagagtg
     5821 agggcaagga gggcaagggc atcttcccgg catcggtgtc attcacaacg gaccttcact
     5881 cgatggggca gtatatccag cagggcgaac gcaaactgtt cgagacggtg attcatgttg
     5941 cagattcata tcacaggctg gttgtgcccg gcgccactga caactctgac ggcatcggct
     6001 tcattgccgg caaaaggatg accgaggtaa accacaaggc tgaggacggc acacgcattg
     6061 cgcatattga cggcggtgta ccagtgatag ttgtcagcat ggaacgcatt gatgaataca
     6121 acctgggcca gctgatgtac ttttttgaac tggcctgcgc catcagcggt tatattctgg
     6181 gcgtcaatcc ctttgaccag ccgggtgtgg agtcatacaa gaagaatatg tttgcccttc
     6241 tgggcaaacc cggcttcgag agtgagcgcg ccaggctgga gcaggatttg cgggataaag
     6301 aatagttcag tatgtgatta cacctgcttc tgcgcctcag actgctgacc ggctgcctcc
     6361 ggtttcatct gcgggaagaa gagcacatcc tgtatagagg gctggttagt catgaacatg
     6421 gtgagcctgt caatgcccat acccattccc gaggtgggag gcatggcata ttccagagcc
     6481 ctgacgaagt ccatgtcgat gaacatcgcc tcgtcatcac ccttttcaga gagacgaagc
     6541 tgatcctgaa acctctcgag ctggtcaatg gggtcattca gctcggagta ggcatttgcc
     6601 agttccttgc cgttgacaat cagctcgaaa cgctcagtta caccatcctt gctgcggtgt
     6661 ttctttgcca ggggtgaggt ctcctgcggg tagtcaatga tgaaggtggg ctggatgaag
     6721 tgatgttcgc acttctcttc gaagatatgg tcaatcagct tgccctttcc catcgtgggg
     6781 gtgtgaccta tctccatcct gtcgcactcc cggcgcaggt catcctccgt catgtccgtt
     6841 atatcaatgc cggcatactc ttttatggca tcggtcatgg caatgcgcct gtagggagcc
     6901 ttgtagtcta taacatgttc acccaggggc accttcgttg ttccatggag gtccatggcc
     6961 accttctcca gtatcttttc ggtgaactcc atcatccaga tgtagtcttt ataggccaca
     7021 tagatctcca tcaccgtaaa ctcagggtta tgggtgcggt ccattccctc gttgcggaag
     7081 tcttttgcaa actcgtagac tccgtcatag ccgcctacta tgaggcgctt caggtaaagc
     7141 tcatccgcta tgcggaggta gagaggtatg ttgagggcat tatggtgagt cacgaatggt
     7201 cttgctgcgg caccgccggg gataggctga agcacgggag tctccacctc gaggtagcca
     7261 caggagtcaa agagctcacg catagacttg attatctgcg tgcgcttgcg gaaaacctcg
     7321 cgcacttcgg gattgatgat caggtcaacg taacgctggc ggtagcgcat ctcctgatca
     7381 gtcaccgcat cgtacagcac cccgtccttc tcctttacta tgggcagagg gcggagcgac
     7441 ttgctgagca gtgtcagctc cttcacatga acggtgattt cgcccatctg ggtacggaat
     7501 gcaaaaccct tcactccgat gatgtcacca atatccagaa gctttttaaa cagggtattg
     7561 tacattgttt tatcctcgcc cgggcagagg tcatcacggc gcagatagat ctgtatgcgg
     7621 cccgatgagt ccatcagctc ggcgaatgag gcgttgccca tgatacggcg gctcatgatc
     7681 cggcctgcca ggcatacatc cgggaattta ttctcagtgt cactgaatcc tgccagtatc
     7741 tctgcagctg aggtgttgac cggatacatt gcagcaggat aagggtctat cccaagtttg
     7801 cgtatctctt caagtgactg cctgcgaacc agttcctgct cactcaaatg tggtgtgctc
     7861 atgctgtaaa aaattt
//
LOCUS       DAOPBK010000125         7834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8463_length_7834_cov_4.272914, whole genome shotgun sequence.
ACCESSION   DAOPBK010000125 DAOPBK010000000
VERSION     DAOPBK010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8463_length_7834_cov_4.272914"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLA17_07980"
     CDS             <1..723
                     /locus_tag="PLA17_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPK85391.1"
                     /translation="TERTLPASEIPEGMVLVPAATIRYKLKAVDEFVPYPGTRKEISA
                     AIDSFLIDRFPVTNEEYLQFIRSSGYIPDDTANYLRHWERGLPVSGQERYPVVWISLE
                     DARAYARWAGKRLPSEAEWQLAAQGTDGREWPWGDEFHATKCNNGFGRPTPVDAFPKG
                     ESPYGVADLVGNIWQMTDEVWCIGAYYYNIIRGGSWFRPESSWWYIKGGPQPLTTTQM
                     QLLVSPGYDRSATVGFRCVRDL"
     gene            complement(893..2077)
                     /locus_tag="PLA17_07985"
     CDS             complement(893..2077)
                     /locus_tag="PLA17_07985"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPK85392.1"
                     /translation="MKSDINRLKEEFFRLYGDRDKDPVLFYSPGRVNLIGEHTDYNGG
                     YVFPCALDFGTTLLVRSGSGEEIRMNSLNFPGEVVIPLALNYLPIPKSWTNYPVGVIN
                     EFARKGIGAPAMEMLFTGDIPNGAGLSSSASIEMVTAVAMNELTGAGLEMMEMVKMSQ
                     KAENGFVGMNCGIMDQFAVGFGRANHAINLNCDTLEYRYAPVNLGDRRLVITNTNKRR
                     GLTDSKYNERRAECEKAVTLISAHRPVRNLSELNVSDLWMLDSYIPDPVVRKRAKHVI
                     TENGRTLDAIKALENNDIELFGRLMNESHDSLCHDYEVTGAELDALVYEGRKLPGVTG
                     TRMTGAGFGGCTVSIVESEHVDEFISRLGKVYTEKTGLTADFYLPGIGDGARMLQAAD
                     NQ"
     gene            complement(2074..3504)
                     /gene="dnaA"
                     /locus_tag="PLA17_07990"
     CDS             complement(2074..3504)
                     /gene="dnaA"
                     /locus_tag="PLA17_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPK85393.1"
                     /translation="MNKSHDEVWNNCLEIIRDIIPSASFKTWFEPIVPLRLEKSILTI
                     QVPSPFFYEYLEEQYIDILRKTLRREIGNDAKLEYNIIMENSGYANGKPYTVRYPSRN
                     KTDLRNKPMQVPFKVTQPAIKNPFIIPGIKKLHLDPRLNPDYSFNNFVEGECNRLARS
                     AGIAVGNNPGGTAFNPLMVYGDSGLGKTHLVQAIGILVKEKYPDKTVLYVNANKFQTQ
                     FVESIRNNNKNDFLHFYQMIDVLIIDDVHEFAGKEKTQDTFFHIFNHLHQSGKQLILT
                     CDKPPVELQGMEQRLLSRFKWGLLADLQKPDFETRLAILRKKAYNDGIELPDDIFEFL
                     ADNISSNIRELESGLISLYAQSTLNRKEVTLELARQMVDRLVNTQRREITIDYIQKVV
                     CEYYKIPVDLIQGKTRKREIVQARQVSMYFSKNLTKASLASIGSCIGGKDHATVLHAC
                     KTVNNLIDTDRHFRNQIREIEKKLKV"
     gene            complement(3827..4768)
                     /locus_tag="PLA17_07995"
     CDS             complement(3827..4768)
                     /locus_tag="PLA17_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPK85394.1"
                     /translation="MKISHEVKVGFVALITIIAFILMYEFLKGTALFTKTDTYHIVYD
                     NIAGLTESNPVEINGYQAGIVQDVQLINDGSGRILVSLSVDKHFNIPRDSKAEITAAS
                     LIAGMKIILRMGESSEMCHNHDTISGYVATSIIDRLGETISPLEGHITSIITRLDSLV
                     SALNTLFTPEMTGDIRSTVANMSDVTADLKQISDNRKDELMAAISEIQTFTAMLSSNS
                     STIDSTLKSISGISETITRADLETSLASLRSSLNGLDTIVTGISRGEGSAGKLVTSDT
                     LYTNLSNSLRSLDLLLRDLKENPKKYVHFSLFGRKTE"
     gene            complement(4779..6098)
                     /locus_tag="PLA17_08000"
     CDS             complement(4779..6098)
                     /locus_tag="PLA17_08000"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPK85395.1"
                     /translation="MPVIVMSQSLPAEDEWVVVIDAGHGGRDPGAVVGKTKEKNINLS
                     VALKTGRYISKNIKDVKVIYTREDDTFLGLDERAEVANRNKANLFISIHSNAMTDKRF
                     SGAETYVLGHTMDEANLQVAMKENSVITFEKDYETKYKGFDPNSVESYIIFSLMQNTY
                     LRQSTEFATLIQNQFRERVGRKDRGVRQAGFQVLWMTTMPSVLVELGFITNAEEEKFL
                     ISEQGQDYLASAIFRAFRDYKQTIDSRSGVRNGKVIAQAATPAAGTDDSTAGRDTQLP
                     SGQQEAKPAVPAAGNESKTADQPARSEIVPSETAVKSNSGQTETAVKSDSGQAEKTVK
                     SESGTAAANPGQATPAPADNIYFMVQVAAMPKNRELTQSQLKGLEPISRIEDGERIKY
                     ASGKFVLYDDALKHRRTLTGRYPDAFVIAVRSGKIMPLKEAIEQQKR"
     assembly_gap    6429..6528
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6529..>7834
                     /locus_tag="PLA17_08005"
     CDS             <6529..>7834
                     /locus_tag="PLA17_08005"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPK85396.1"
                     /translation="DSATVLMTLPDTVVKSDSGTILKSLPDTLVKSDSGTILKSLPDT
                     SVKSDSGALSKTLPDTLVKSDSGALPKTLPDTVVRSDSGTILKSLPDTLVKSDSGTLP
                     KTLPDTSVKSDSGTLPKTLPDTVVTGLNEEDLSPAFVNDTLFPGTSGTITDTSLTKTG
                     LIISSDAVDEPIVYNSDGYMKTDMRSKKVSLVENARVTYGTIQLTADSIVLDMETGSV
                     FATFRRDSTGKAVGLPVYKDGSEEFESEELNYNFKSKKGVIRNVTTEQEGGFLQSLTT
                     KRHDDGTLHVNRSKFTTCDADEPHFYLSLPRAKVYPGEKIVSGPAYMVVADIPLPLVL
                     PFGFFPVQQKRASGIVMPKYGQEARRGYFLSNGGYYFALSDYFDLKLTGTLYTNGTWL
                     ADAATSYRLRYRFSGSFSFSYANNVTSYKGLPDYRKNTNYRIS"
BASE COUNT         1781 a         1966 c         1871 g         2116 t
ORIGIN      
        1 actgaacgca ccctgcccgc atctgagata ccggaaggga tggtgctcgt ccccgccgcc
       61 acaatacgat acaagctgaa ggctgtagat gagtttgttc cctatcccgg aacgaggaag
      121 gagataagcg ccgctattga cagtttcctg attgacaggt tccccgtgac gaacgaggag
      181 tacctgcagt ttatcagatc atcggggtat attcctgacg atactgccaa ttacctgaga
      241 cattgggaga ggggactccc cgtcagcggc caggaacgat atcctgtcgt ctggattagt
      301 cttgaggatg ccagggctta tgcccgctgg gccgggaagc gcctgccgtc agaagcagag
      361 tggcagcttg cggcacaggg caccgacggc cgggagtggc cgtggggaga cgagtttcat
      421 gccaccaaat gcaataacgg ttttggcagg cctaccccgg ttgatgcctt cccgaaaggg
      481 gagagccctt acggagtggc tgatctggtc ggcaacatat ggcagatgac agatgaggtc
      541 tggtgcatcg gagcctatta ctacaatatc atcaggggag gaagctggtt caggcccgaa
      601 tcaagctggt ggtatataaa gggcggaccg cagccgctga caactaccca gatgcaactc
      661 ctcgtctcac ccggttatga ccgaagcgct accgtcgggt tccggtgcgt gcgtgatctt
      721 tgaccagggg caaacgccgg cttttacagt attgcaagtc tcgttcaggt gtgtgcgtta
      781 tctctctccg gcaagagtac tgaggtaagg tggtcaaaaa cagttggtgt ttttcaagga
      841 ttgacaatac agattataaa tacttgagta acagtaactt aatagttaac tgttactgat
      901 tgtctgcagc ctgcagcatg cgtgccccgt caccaatccc cggcaggtaa aaatcagctg
      961 tgaggccggt cttttcggtg taaactttac caagcctgct gatgaattcg tcaacgtgtt
     1021 ccgactctac aattgacacc gtgcatccgc caaatcccgc tccggtcatc ctggtgcccg
     1081 tcacgcccgg gagcttcctt ccttcgtaaa cgagggcgtc aagctcggca ccggtcactt
     1141 cataatcatg gcagagtgag tcatgcgact cgttcatcag tctcccgaaa agctcaatgt
     1201 cattgttctc cagggccttt attgcatcca gtgttctccc gttttcagta attacatgtt
     1261 ttgccctttt tctgactacg gggtctggaa tataactgtc aagcatccac aggtcgctga
     1321 cattcagctc actcaggttg cgcacaggcc tgtgtgctga gataagtgtc acagcctttt
     1381 cgcattcggc acgtcgttcg ttgtacttgg aatcagtcag tcccctccgt ttgttggtgt
     1441 tcgtgatgac aagcctcctg tcacccaggt tgacaggtgc ataccggtac tccagagtgt
     1501 cacagttaag attgattgca tggttggccc tgccgaaacc caccgcaaac tggtccatga
     1561 tgccacagtt catccccacg aatccgtttt cggccttctg gctcattttc accatctcca
     1621 tcatctccag tccggcgccg gtgagctcat tcatggcaac cgccgtcacc atctcaattg
     1681 aggcagagga ggagagtccc gcaccgttgg gtatgtctcc ggtgaagagc atctccattg
     1741 ccggagcccc gatgcccttg cgggcaaatt cattaatgac cccgacgggg tagttggtcc
     1801 agctctttgg gataggcaaa taattgagag ccaatggtat caccacctct cccgggaagt
     1861 tgaggctgtt cattcttatc tcctctccgc tgccggagcg cacaaggagg gtagttccga
     1921 aatcaagtgc acacgggaat acatatccgc cgttatagtc agtgtgttcg ccgatgaggt
     1981 tgaccctgcc gggtgaataa aaaaggacag gatctttgtc cctgtccccg tataacctga
     2041 aaaactcttc cttcagtctg ttaatgtctg atttcatacc ttaagctttt tctctatttc
     2101 ccttatctgg ttgcggaaat gacggtcggt gtcaatgagg ttgttcacgg tcttgcaggc
     2161 atgaagcacc gttgcgtgat ccttccctcc gatacaggaa ccaatactgg caagcgatgc
     2221 cttggtcagg ttcttggaga aatacattga tacctgcctg gcctggacaa tctccctctt
     2281 gcgggtcttg ccctgtatca ggtcaacagg aatcttgtaa tactcacaga caaccttctg
     2341 gatgtaatca atcgtgatct ccctgcgctg ggtgttcaca agcctgtcaa ccatctggcg
     2401 cgcaagctcg agtgttacct ccttgcggtt gagggttgac tgtgcataga gagagataag
     2461 acctgactca agctcccgta tattcgatga aatattatct gcaaggaact cgaaaatatc
     2521 atccggcagc tcaatgccgt cattgtatgc cttcttcctc aagatcgcca gcctggtctc
     2581 aaaatcaggc ttctggagat cggccagcag cccccactta aaacgcgaga gaagtcgctg
     2641 ctccataccc tgaagctcta ccggcggctt gtcacaggtc agtatcagct gcttacccga
     2701 ctgatggagg tggttgaata tgtggaagaa agtgtcctgt gtcttctcct tgcctgcaaa
     2761 ctcatggacg tcatcaataa tcagaacgtc aatcatctgg taaaaatgga gaaagtcatt
     2821 cttgttgttg ttccttattg actctacaaa ctgggtctgg aatttattgg catttacata
     2881 cagaacggtc ttgtcaggat acttctcctt aaccaggatg cctatggcct gtaccaggtg
     2941 agtcttgccg agcccggaat caccgtagac catgagcgga ttgaaggcgg tgccccccgg
     3001 attgtttcct acggctatgc ctgctgaacg ggccagacgg ttgcattcac cctctacaaa
     3061 gttgttgaag gagtagtcgg gattaagcct tgggtcaaga tgaagcttct tgattcccgg
     3121 tatgataaac ggattcttta ttgcaggctg ggtgacctta aagggaacct gcatcggttt
     3181 gttcctgaga tcagtcttgt tccttgacgg ataccttacc gtgtaaggct tgccattggc
     3241 gtatccggaa ttttccatga taatgttgta ctccagcttg gcatcattgc caatctcacg
     3301 cctcagggtc ttgcggagga tgtctatgta ctgctcttca agatattcgt aaaagaaagg
     3361 gcttggcacc tgtattgtaa gaatgctttt ctcgagcctg agcggaacaa taggctcgaa
     3421 ccaggtttta aaactggccg aaggtataat gtctcggatt atctcgagac aattattcca
     3481 aacctcatcg tgtgatttgt tcattcagtt agaaattaaa aaaaagtagc ttttttctgg
     3541 tgctgaaacc cgtcacaata tttgcaaaaa aaaggtaaaa aaaaaatttt tttgctcttg
     3601 actttgccat ttattctata ctgtaatgaa cctcaatccc ttaaaatgac aaacattggt
     3661 caaaatgtct aattttcaca ttatcaatat attagtatga acgagatgtc gctcagtctt
     3721 ttacaaattg aagatattct tatcctgaaa tgccctgtca ggtcaaattc caatcatttg
     3781 gcctgcaagg cggtgatatt gtattgatta atattattta aacaaatcat tccgtttttc
     3841 tgccaaaaag ggaaaaatgg acatatttct ttggattttc cttcaggtca cggagtaaaa
     3901 ggtcaagact tctgagtgaa ttgctcaggt ttgtgtagag agtgtcactt gtgacaagct
     3961 tccctgccga accttctccc cggctgattc cggttacgat tgtatcaagt ccgttcagcg
     4021 atgaacggag ggaggcaaga gaggtctcca ggtcagccct ggtgatggtt tcggagatac
     4081 cgcttatact cttaagggtg gaatcaattg ttgaactatt ggatgaaagc atcgcggtga
     4141 aggtctgtat ttcactgatt gcagccatga gctcatcttt cctgttgtca gatatctgct
     4201 tcagatcagc ggtaacatca ctcatgtttg ccacagtgga acggatatca ccggtcattt
     4261 caggcgtgaa gagagtgttg agggctgaca ccaacgagtc tagcctggtg attatacttg
     4321 tgatgtggcc ttccagcggg ctgatggtct cacccagcct gtcaataatt gatgtggcca
     4381 cataccctga gattgtgtca tggttatggc acatttcgct gctttctccc atgcggagga
     4441 ttatcttcat gccggcaatg agggaagccg cggttatctc agccttcgag tccctgggta
     4501 tgttaaagtg cttgtcaaca gaaagtgata ccagtattcg ccctgacccg tcattgatca
     4561 gttgtacatc ctgtacgata cccgcctggt aaccgttgat ctctaccggg tttgactctg
     4621 tgagtccggc aatgttgtca taaacaatat gataagtgtc ggtttttgtg aagagggcag
     4681 tgcccttgag aaactcgtac atcaggatga atgcgataat ggtgatcagt gctacgaaac
     4741 cgacctttac ttcgtgggat atcttcatag ggttgctttt atctcttttg ttgttctatc
     4801 gcctccttca ggggcataat ctttccgctc ctgacagcaa tgacgaatgc gtcggggtat
     4861 ctacccgtca gcgtgcgtct gtgcttcagt gcatcatcat ataaaacgaa ttttcctgat
     4921 gcatatttaa tcctttcgcc atcttctatc ctgcttatcg gttcaagccc ctttaactgg
     4981 ctctgtgtca gctctctgtt ttttggcatc gcggctacct gcaccataaa ataaatattg
     5041 tctgccgggg caggagtggc ctggccgggg tttgccgctg ccgtaccgct ttcgctcttg
     5101 actgtctttt ctgcctgccc gctgtcactc ttcacagctg tttccgtctg cccgctgttg
     5161 ctcttcacag ctgtttccga aggaacaatc tcactcctgg ccggctgatc cgcggttttg
     5221 ctttcattcc ctgcagctgg taccgcaggt ttggcctcct gctgccctga tggcaactgt
     5281 gtgtctctgc cagccgtgga gtcatcagta cccgctgccg gggttgctgc ctgtgcaatc
     5341 actttgccgt ttcttactcc tgacctgctg tcaatggttt gtttgtagtc acggaatgcc
     5401 ctgaaaattg cagaggcaag ataatcctgt ccctgctctg atatgaggaa cttctcttcc
     5461 tcggcattgg ttatgaaacc cagttccacc agcactgaag gcattgtcgt catccagagt
     5521 acctggaacc ctgcctgcct gacacccctg tccttcctgc caaccctctc cctgaactgg
     5581 ttttgtatca atgtggcgaa ttcggtgctc tgcctgaggt aggtgttctg catgagtgag
     5641 aaaatgatgt atgactccac cgagttggga tcgaaacctt tgtacttcgt ttcgtagtcc
     5701 ttttcaaagg tgatgactga gttttccttc atcgcaacct gcaggttagc ctcatccatc
     5761 gtatgaccca ggacataggt ttccgccccg ctgaacctct tgtcagtcat ggcatttgaa
     5821 tggattgaga taaacaggtt tgccttgttc ctgtttgcca cctcagccct ctcgtcaagc
     5881 ccgaggaaag tatcgtcttc acgagtgtag atgaccttca catccttaat gtttttacta
     5941 atatacctgc ctgtcttgag cgctacagag aggttgatgt tcttctcctt tgtcttccct
     6001 acgacagctc cggggtctct ccctccgtga ccggcatcaa tgaccactac ccactcatct
     6061 tcagccggga gcgactgtga cataacaata accggcatac taattgctgc tacaaacagg
     6121 gtatacgccg gaataactct taaccctctt attttggtag tttgccgcat tactgctttg
     6181 cgaattttaa ttagttttgt cgctggctct tcagagatgc tcattaatga cgcaaaaata
     6241 gcatttaact tgcattcttt tccacaaaga gtgtttgtga caattttcat gccgcttctt
     6301 ttacaggcca cattgcttgc ccaggagccg gggcggagtg tcacaaccgg aaagagcctc
     6361 cctgacacac tgttgatgac cctgcctgat accgttgtga agagtgattc cgccaccgta
     6421 ctgatgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ttccgccacc
     6541 gtactgatga ccctgcctga taccgttgtg aagagtgatt ccggcaccat actgaagagc
     6601 cttcctgata ctttagtgaa gagtgattcc ggtaccatac tgaagagcct tccggatact
     6661 tcagtgaaga gtgattccgg cgccctgtcg aagaccctcc ctgatacttt agtgaagagt
     6721 gattccggcg ccctgccgaa gacccttccg gataccgttg tgaggagtga ttccggcacc
     6781 atactgaaga gccttcctga tactttagtg aagagtgatt ccggtaccct gccgaagacc
     6841 ctcccggata cttcagtgaa gagtgattcc ggcaccctgc cgaagaccct tccggatacc
     6901 gttgtgaccg ggctgaatga agaggatctt tctcctgcgt ttgtcaatga cacactcttt
     6961 cccggtacat ccggaacaat cactgatacc tcgctgacca aaacaggcct gattatttct
     7021 tcagatgctg ttgatgagcc aattgtctat aattctgatg gttacatgaa gactgacatg
     7081 aggtcgaaga aagtcagcct tgtggagaat gccagagtca cctacggaac catacagctc
     7141 acagctgact ccattgtgct ggacatggag acaggatcgg tattcgccac cttcagaagg
     7201 gattccaccg gcaaggctgt aggcctgccc gtctacaagg atggctcgga ggagttcgaa
     7261 tcggaagagc ttaactataa cttcaaatcg aaaaaggggg tgatccgtaa tgtcaccacc
     7321 gagcaggagg gaggcttcct tcagagcctg acaacaaaga ggcacgatga cgggacgctt
     7381 catgtcaacc gcagcaagtt caccacctgt gacgctgatg agcctcattt ctatctctcg
     7441 ctcccgagag ccaaggttta cccgggagag aaaatcgtgt caggacctgc atatatggtt
     7501 gttgccgata ttccccttcc tctagtcttg cctttcggat tcttcccggt acagcagaag
     7561 agggcatccg gaattgtcat gcccaaatac ggccaggaag cacggagggg gtatttcctt
     7621 tccaacgggg ggtactattt cgcgttaagc gactactttg acctgaaact gacgggtact
     7681 ctttacacaa acggcacatg gcttgccgat gcagccacct cataccggct tcggtaccgc
     7741 ttttcgggat cattcagttt cagttacgcc aacaacgtta ccagctacaa gggactgccc
     7801 gattacagga agaataccaa ttaccgcatc agct
//
LOCUS       DAOPBK010000126         7791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8507_length_7791_cov_5.215098, whole genome shotgun sequence.
ACCESSION   DAOPBK010000126 DAOPBK010000000
VERSION     DAOPBK010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8507_length_7791_cov_5.215098"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="PLA17_08010"
     CDS             complement(<1..1863)
                     /locus_tag="PLA17_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPK85397.1"
                     /translation="MVKTRTKIFTADDISRIETEYNLLTDNLYRCDNPGDRELIDKAF
                     RFANEAHRDMYRNSGEPYIIHSISVARIVNQEIGLGARSVSAALLHDVVEDTEIPLEE
                     ISREFGPKIASLIDGLTKISGTYNKETNSLQAENFRKMLMTLSDDFRVILIKIADRLH
                     NMRTLDSMPEHKRMKIAGETVYLYAPLAHRLGMFAIKTELEDLSFKFRHPRIYDEIAR
                     KVKMDEKKNLALINKFSLPIIEALNDNNILFDISGRFKSIYSIWKKMQSKNIPFEEVF
                     DLLAVRIVFEPGPEMSERAQCWQIYSLITDIYTPKPDRLRDWISRPKSNGYEALHLTV
                     MGPEGRWVEVQIRSQRMDDIAERGFAAHWKYKGDMSQTESELDKWLKHIREMLANPMS
                     DPLSFLDEFKMSLFSAEIMVFTPKGLLVNLPKGASALDFAYEIHTDIGNKAIGAKVNY
                     KLSPLSTTLQSGDQVEIITSNIARPEKEWLEYAHTAKARNAIKDALRDESTDRIQKGM
                     EMLEKKLSELGILPNSDIIKKLLDYYDLLNKDELYSRIGLGLINLDELRKVLKKAPEG
                     SLRKYWKLATGGANRKNPGTRKAPARAEKIDTSQHYLLKENVDSNDRSYGIGSSC"
     gene            complement(1924..3399)
                     /locus_tag="PLA17_08015"
     CDS             complement(1924..3399)
                     /locus_tag="PLA17_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPK85398.1"
                     /translation="MKKQNSLIKLFILLLLCSLSALPLAGQDDERPQSPTLDMVTVDP
                     ATGFALLKWLPSPSPDVGSYVVYIYTEERADAIDTIYSPFVFEYTHTASVARYRSVSY
                     VVAAIDSSDNPSPLSNSLSTIWLSAAEDPCTATVTVSWTNYDNQFHPGSGYRLHITDG
                     TGGSYPEVVLPLSSTQYLFTGYEADTEYCFHVTASDGTVPLSSSNRACITTGSEVAPE
                     WVRIYAIAVDNDGISFSAGYDPSTVMNDFRLFRHDPVEAAWDEAASSTGVAGRVSFFM
                     AQADTTVVRLYRVAAMNSCDLASTVSAPSRNMVPEASISGTVVNLRWNRPSAGWSGLF
                     SVWRDTGDGMREVVSSLSDTLWSDDYTTFATDVSAAEVVYRVSTTDLSAPAGTGPHLS
                     SAAVIEASENVFMPNAFTPGSTDENAIFRPEFSFHPGEYDFRVVSRSGVLLFRTGDPG
                     EGWDGRHNGRMMPPGVYLWSLRLTTTSGQTLERNGTVTILP"
     gene            complement(3396..4682)
                     /locus_tag="PLA17_08020"
     CDS             complement(3396..4682)
                     /locus_tag="PLA17_08020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK85399.1"
                     /translation="MRDKEIKGAPKIITPSAGDTSLPEPVMLGNGVPVYLAGNGTVDL
                     LRIEFVFPAGQVLEEVHLAASTTSAMLTEGTLHHDAATINDLIDSTGATLSHAADKDT
                     AGLTTVTLTRKLNDVMALASEVLFSPSFPENEFRILTDRRLQAFQTSRQKTPVIAREA
                     FYEALCGAGSPYGRITTEDDYRRLTTGDLREFHRKHYRPVNMYVTVAGSEPWKALPVL
                     EKHFSVNGTEQWQKPPLPSPLLETSEPGKIFREVPSSVQSTIRVGWKGITRNHPDYQA
                     LQVATVILGGYFGSRLMRTIREEKGYTYGIGAVAGAFHNIGYITIMTDVANAYRDDTL
                     REIRKEVEKLRTEEVSGEEMTLVRNHMMGGLARAFDGPFLVADALRSVIDYETGSDYY
                     TRLAETVKTITPARIMDIFDKYFNPDEAWEIIAGSR"
     gene            complement(4787..6040)
                     /locus_tag="PLA17_08025"
     CDS             complement(4787..6040)
                     /locus_tag="PLA17_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK85400.1"
                     /translation="MIRFERQKLANGLRVIVHRDESTPMVTTNILYDAGSRDEDPDST
                     GFAHLFEHLMFEGTRNVPLFDVPVMMSGGENNAFTNNDLTNYFITLPATNLETALWLE
                     SDRMRGLDLAEERLANQKSVVIEEFRQRYLNQPYGDLLLLLRPLAYKVHPYRWPAIGM
                     DISHIEKADSISARKFYDRFYIPSNAILSVAGNVDPDRAFRLTEKWFGDIPSGLKSER
                     NLPVEPEQKERRELTVYRPVPSAHLIMAFHMGSRMSRDFYILDLLTDILAGCDSALFP
                     AILVRKKELFSEADIYLSGEADPGLVIFSGRIRDGIDIRKAEEAVWSELDRLAAEPVK
                     AADLDKARNRFESQQLMSYVSVANKAFGLACHEVLGDADGINREQEQYMSVTAEEIAS
                     IAGLTFRSGNCSVINYLPGKRRETT"
     gene            6136..>7791
                     /locus_tag="PLA17_08030"
     CDS             6136..>7791
                     /locus_tag="PLA17_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPK85401.1"
                     /translation="MKNLLIRKIGQIKALLTNQQTLIYILSFLIGLVSALAAVVMKNA
                     VHFTHVLFTQGVMKGAGGVLVLAYPLVGIFLTVLVVKYLVRDNISHGISRVLYAISTR
                     KSYIKPHNNWSSIVTSSLTIGFGGSVGVEAPIVLTGASIGSNIARHFKLNYKNVTLML
                     GCGVAGAISGIFKAPIAGILFTLEILMLDLTMSSIVPLLISSVTAATVAFFFMGDNVL
                     FSFHVKQGFMLSELPYYLILGVFCGLVGLYFTRATIGIEKLYKKIGNKWIRMLVGGSI
                     LGILIYFYPALYGEGYETVMALLNAGTGVAPGKEIFGAVSTGFWSFTLLMAGLLLVKV
                     VATASTNGAGGVGGIVAPVLFTGGVAGYFLSGLLNQLFGLALIESSFTLAAMAGVFSA
                     VLHAPMTAIFLIAEITEGYGLLIPLILTSTVAFITARSFQKYSIYTEQLALSGDLITH
                     NKDKAVMTLLDWSKEVEKDLLTVRPDGTLRDLVKQISKSNRNLFPVVDEFNILEGVVL
                     LDDVRDKMFNPELYDKLTVRELMAIPPSYIDISERGDAVLDAFE"
BASE COUNT         1797 a         2055 c         1884 g         2055 t
ORIGIN      
        1 acagctactg cctatgccgt atgatctgtc attgctgtcg acattttcct tgagcaggta
       61 gtgctgactc gtgtcaattt tttcagcacg ggcaggcgcc ttccttgttc ccggattctt
      121 gcggttagca cctcctgttg ccagcttcca gtacttgcgc agacttcctt caggagcctt
      181 cttcagcact ttgcgcaact cgtcgaggtt gatcagcccc agcccgatac gcgaatagag
      241 ctcgtccttg ttgagcagat cataataatc cagaagcttc ttgatgatgt ctgagtttgg
      301 aagaatcccc agttcactca gcttcttctc aagcatctcc atccccttct gtatgcggtc
      361 agtagattca tcgcgaagtg catccttgat cgcattcctg gctttcgccg tatgggcata
      421 ctcaagccac tccttctcag gcctggcaat attggatgtg ataatctcaa cctggtcacc
      481 gctctgaaga gttgtggaga gaggggagag cttgtagttt accttggcgc cgatggcctt
      541 gttgcctatg tcagtatgga tttcatacgc gaagtcaagg gcagaagcgc ccttgggaag
      601 gttgaccagc agtccttttg gggtgaatac cattatctcc gcggagaaga gactcatctt
      661 gaattcatcg aggaagctga gcggatcaga catcgggttg gcaagcatct cccggatatg
      721 cttcagccat ttatcgagtt cactctcagt ctgagacata tcacccttgt acttccagtg
      781 agccgcaaac cctctctcag caatatcgtc catcctctga ctacggatct gcacctcaac
      841 ccatcgcccc tcagggccca tcacggtaag atgaagcgcc tcataaccgt ttgacttggg
      901 cctgctgatc cagtcacgca gcctgtcagg cttaggtgtg tatatatctg ttataagtga
      961 atatatctgc cagcactgtg ccctctcact catctccgga ccgggctcga agacaatcct
     1021 tacggcaaga agatcaaata cctcctcgaa cgggatattc ttcgactgca tcttcttcca
     1081 gatggaatag attgacttga agcggcctga tatgtcaaac aggatgttgt tgtcattcag
     1141 cgcctctatg attggcaggc tgaacttatt aataagggca aggttctttt tctcatccat
     1201 ttttaccttg cgcgcgatct cgtcatagat gcgcggatgc ctgaacttga agctcaggtc
     1261 ttccagctcg gtcttgatgg caaacatccc gagccggtgt gccagtggcg catagagata
     1321 tacagtctct ccggcaatct tcatcctctt gtgctcgggc atcgagtcaa gagtacgcat
     1381 gttgtgcagc ctgtcagcta tctttatcag tatgaccctg aaatcatccg agagggtcat
     1441 cagcatcttg cggaagttct cagcctgcag tgagttggtc tccttgttat aggtccctga
     1501 tatcttggtc agaccatcaa tcagagaggc gatctttgga ccgaactccc tggatatctc
     1561 ctcaagggga atttcggtat cttccactac atcatgcaac agcgcggctg aaactgacct
     1621 ggcacccagt cctatctcct ggttgactat ccgggctaca ctgatcgagt gaatgatgta
     1681 cggctccccg gaattgcggt acatgtcacg atgtgcctca ttcgcgaacc tgaatgcctt
     1741 gtcaatcaat tccctgtcac caggattatc acacctgtag agattatcag tcagcaggtt
     1801 gtattctgtc tcgatacggc tgatatcatc agcggtgaat attttggtcc tggtcttaac
     1861 catcagtctc tacctgcaaa caagtaacaa agatacgaaa atcgactcat tattatttag
     1921 ctgttatggc agtatagtta cggtcccgtt cctttccagg gtttgtcctg acgttgtggt
     1981 aagcctcagg ctccagaggt acactcccgg cggcatcatc ctgccgttgt gcctgccatc
     2041 ccatccttcg ccggggtcac ccgtgcggaa caacagtaca ccgcttcttg aaactaccct
     2101 gaaatcatat tctcccgggt gaaaggaaaa ctcaggcctg aaaatggcgt tctcatccgt
     2161 actgccgggg gtgaaggcgt tgggcatgaa aacattctca ctggcttcaa ttactgcagc
     2221 agaggagagg tgcggcccgg tgcctgcagg cgctgagaga tcagttgttg atacccggta
     2281 aaccacttcg gctgctgaca catctgtagc aaaggtggtg taatcatccg accagagtgt
     2341 gtcagacagg gatgagacca cttcacgcat gccatctcct gtatcacgcc agacactgaa
     2401 cagcccgctc catccggcag agggcctgtt ccaccgcagg ttgacaactg ttccgctgat
     2461 tgatgcttcc ggcaccatat tccgggatgg cgctgaaaca gttgaagcca gatcacaact
     2521 gttcatggct gcaaccctgt acaacctgac aacagtggtg tctgcctgtg ccatgaaaaa
     2581 tgagactctg ccggcaactc ccgtcgaaga ggcagcctca tcccatgcag cttcaacagg
     2641 atcatggcga aaaagcctga aatcattcat caccgttgac gggtcatagc cggcagaaaa
     2701 tgatatgccg tcattatcta ctgcaattgc atatatcctt acccattccg gggcaacctc
     2761 gctgccggtt gtgatacagg ccctgtttga cgatgaaagt gggacagtac catcacttgc
     2821 cgttacatgg aaacagtatt ccgtatccgc ttcgtacccg gtgaacaggt actgcgtgga
     2881 ggatagaggc agtactacct caggatacga tcctcccgtt ccgtctgtta tatgcaaccg
     2941 gtaaccgctg ccagggtgaa actgattgtc ataatttgtc catgagactg ttacggttgc
     3001 agtgcacgga tcctcagcgg ctgagagcca gatggtgctg aggctgttgc tgaggggact
     3061 cggattgtct gatgagtcaa tggctgccac cacgtatgac acactcctgt acctggcaac
     3121 tgaagcggtg tgggtatatt caaaaacgaa tggtgaataa atggtatcaa tggcatcagc
     3181 cctttcctca gtatagatgt agacaacata actgcccaca tccggtgaag gggaaggaag
     3241 ccacttcagg agtgcaaacc cggttgcagg gtcaactgtc accatgtcaa gagttggtga
     3301 ctggggccgt tcgtcatcct gtcctgccag tggcaaggca gacaaactgc acaggagcag
     3361 caggatgaag agcttaatca gggaattctg ctttttcatc ggcttccggc aataatctcc
     3421 caggcctcat ctggattgaa atacttatcg aagatatcca ttattctggc tggcgtaatt
     3481 gttttgacag tttctgctaa acgtgtataa tagtctgatc ctgtctcata gtcgatgacg
     3541 ctcctgagag catcggcaac caaaaacgga ccgtcgaagg cccgggcaag ccctcccatc
     3601 atgtgatttc tgaccagggt catctcttcc ccgctaacct cttcggtgcg gagcttctca
     3661 acctctttcc tgatttcacg cagagtatca tccctgtagg catttgccac atcggtcatg
     3721 atggtgatat agcctatgtt atggaaagca ccggctactg ccccgatgcc gtacgtatag
     3781 cccttctcct cgcgtatggt acgcatcagg cgcgagccga aatacccgcc gagtatcact
     3841 gtggctacct gcagggcctg gtagtcagga tggttgcggg taataccctt ccagcccacc
     3901 cgtatggtgc tctgaaccga agagggaact tcacggaaaa tttttccggg ctccgaagtt
     3961 tccaacaggg gagatgggag aggaggcttc tgccattgtt ccgttccgtt gactgagaaa
     4021 tgtttttcca gtacgggcag cgctttccat ggctcactcc cggcaacagt cacatacatg
     4081 ttcaccggcc tgtagtgctt ccggtgaaac tctctcaggt cacctgtcgt gagtctccgg
     4141 taatcatcct cagtggtaat gcgtccgtaa gggcttccgg caccgcacag agcttcatag
     4201 aaagcctccc tcgcaatcac cggggtcttc tgcctgctgg tctgaaaagc ctgtaacctt
     4261 ctgtccgtta atattctgaa ttcattttca ggaaaggaag ggctgaagag cacctcactg
     4321 gcaagggcca tcacatcatt aagctttctg gtcagggtga cagttgtcag tccggctgta
     4381 tccttgtcag cagcatgtga cagtgtggct ccggtgctgt caatgagatc attgattgta
     4441 gctgcatcat gatgcagggt gccttcggtg agcattgcac tcgtagtgga ggctgccaga
     4501 tgaacctcct ccaatacctg gccggcaggg aaaacaaact caattctcag cagatcgacg
     4561 gtgccattgc ccgccaggta taccggcacg ccgttgccaa gcatcaccgg ttccggcagt
     4621 gaggtatctc ccgctgacgg agtaataatc ttcggggctc cctttatctc tttgtctctc
     4681 atccctttac tttgtctctc gtctcttcac tttttctctt atcctttcac tttgtctctc
     4741 acctcttcac gttgtctctt gtctcttcac tttgtctctc acctcttcac gttgtctctc
     4801 gtctcttccc gggcagataa ttgattacgc tgcagttccc tgacctgaat gtcaggccgg
     4861 caattgaggc tatctcttct gccgtcacac tcatgtactg ttcctgttcc ctgttgatgc
     4921 cgtcagcatc gcccaatacc tcatgacagg caagtccgaa tgctttgttg gcaacgctga
     4981 cgtatgacat cagctgctgt gactcaaatc tgttacgtgc cttgtcaagg tctgcagcct
     5041 tgacaggctc agcagccaac ctgtcaagct ctgaccagac tgcttcctca gccttcctga
     5101 tatcgatccc gtctctgatc ctgccactga atatgacaag acccggatca gcttcgcctg
     5161 agagataaat atcggcttcg ctgaatagct ccttcttcct gacgagtatg gcagggaaca
     5221 gcgcggagtc acatccggca agtatatcgg taaggagatc aaggatgtaa aagtcacggc
     5281 tcatcctgct tcccatgtga aaggccataa tcaggtgagc ggaaggaaca ggtctgtaaa
     5341 ccgtcagctc gcgccgttcc ttctgttccg gctccacggg gagatttctt tcactcttca
     5401 gacctgacgg aatatctccg aaccattttt ctgtaagcct gaatgccctg tccggatcaa
     5461 cgtttcctgc aacactgagt attgcattgg aggggatgta aaaacggtca taaaactttc
     5521 ttgccgagat gctgtcagct ttttcaatat ggctgatatc catccctata gccggccacc
     5581 ggtagggatg aaccttatag gccaggggcc tgagcaacag cagcagatcg ccgtatggct
     5641 ggttaagata gcgctgcctg aactcttcga tgacaacact cttctggttg gcaagcctct
     5701 cctctgccag gtcgaggccc cgcatcctgt ctgactcaag ccataacgcc gtctcaaggt
     5761 tggtggcagg aagggttata aagtagttag tgaggtcatt gttggtgaag gcattgttct
     5821 caccgccgga catcataaca ggcacgtcaa aaagaggtac gttccgcgtc ccctcgaaca
     5881 tcaggtgttc aaagagatgg gcgaaacctg tggaatcagg gtcctcatcc cttgagcctg
     5941 catcataaag aatattggtt gtcaccatgg gagtactctc atcccggtgt actatcaccc
     6001 tcagcccgtt ggcaagcttt tgcctttcaa atctgatcat atatgcaaaa taggtcaaac
     6061 ttaccaaaat aaacatcaga tattcatttt ataatatatt tgtatcttaa tgtttttcat
     6121 ttttttccgg taaccttgaa gaacctgtta attaggaaaa tagggcagat aaaagctctc
     6181 cttaccaacc agcagaccct gatttacatt ctcagcttcc tgattggcct cgtcagtgcg
     6241 ctggcagccg tcgtgatgaa gaatgcagtt cactttacac acgttctgtt tacccagggt
     6301 gtcatgaaag gtgccggagg agttctcgtg cttgcctatc cgctggtagg gatatttctt
     6361 acagtgctgg ttgtcaagta cctcgtcaga gataatatct cacatgggat cagcagggtg
     6421 ctctatgcta tctctacccg gaaaagctac ataaaacccc ataacaactg gtcgtcaata
     6481 gtgaccagct cgctcacgat aggctttggc ggctcggttg gagtagaggc tcccatcgtt
     6541 cttacaggcg cttcgatagg ctcaaacata gcacgccatt tcaagctgaa ttacaagaat
     6601 gtgaccctga tgctcggatg tggcgttgcc ggggctattt ccggaatatt caaggctccc
     6661 atcgccggaa tactattcac cctcgagatt ctgatgcttg acctgacgat gtcatcaata
     6721 gttccccttc tgatctcatc ggtgactgca gcaaccgtgg catttttctt catgggtgac
     6781 aatgtgctgt tctcctttca tgtaaaacag ggcttcatgc tgagcgagct gccttactac
     6841 ctgatactgg gagtcttttg cggcctggtc ggattatact ttacaagggc aactatcgga
     6901 attgaaaaac tctacaaaaa gattggcaac aaatggatta ggatgctggt aggcggatcg
     6961 attctcggaa tcctgatcta tttctatccg gcgctctatg gtgaagggta cgaaacagtg
     7021 atggcgctcc ttaatgccgg aaccggtgtt gcacccggaa aggagatctt tggcgctgtt
     7081 tccaccggat tctggtcctt cacgctgttg atggccggac tgctgcttgt caaggtggtg
     7141 gcaaccgcct ccaccaacgg tgccggggga gtaggaggca tagtggctcc ggtactgttt
     7201 accggaggag tagcaggata ttttctctcc ggcctgctga atcaactgtt cggcctggcg
     7261 ctcatcgaaa gcagtttcac ccttgctgcc atggcaggag tcttctcggc cgttcttcat
     7321 gcacccatga ccgccatctt cctcattgcg gagataacgg aaggttatgg cctgctcata
     7381 cccctgatac ttacatctac cgtagcattt atcactgcca gatcattcca gaagtattcg
     7441 atttacactg aacagcttgc actaagcgga gatctgatca cccataacaa ggacaaggcg
     7501 gttatgactc tgctagactg gtcaaaggag gtggaaaaag acctgctgac agtgagaccc
     7561 gacggcaccc tgagagatct tgtgaagcaa atctcaaaat caaaccggaa cctcttccct
     7621 gtggtggatg agttcaatat cctggaggga gttgtgctgc ttgatgacgt ccgtgacaaa
     7681 atgttcaacc ccgaactcta tgataaacta accgtaagag aactgatggc cataccacct
     7741 tcatatattg atatcagtga gagaggtgat gcagtccttg acgcctttga a
//
LOCUS       DAOPBK010000127         7765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8536_length_7765_cov_6.151881, whole genome shotgun sequence.
ACCESSION   DAOPBK010000127 DAOPBK010000000
VERSION     DAOPBK010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8536_length_7765_cov_6.151881"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="PLA17_08035"
     CDS             <1..1382
                     /locus_tag="PLA17_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPK85402.1"
                     /translation="GVHYATRSGYHTGCAECHLPPKGEGYLVEKTKTGLRDLWSHWTK
                     DSADFDWEAASALERASKHVYNSSCIYCHENLFPAQLTTEGSEAHLYYKQMTEKGEDL
                     QCISCHLNAGHYIEGYFHGSNTGFGASVTRVDTLYQYPATVTGFEDFTEQIPGSAVTF
                     RMIAVPGGEFIMGSPGGEPLRGEDEAEKKVKVDSLFIAEVEVTWDEYMEFYRQTASEG
                     RSTDTEGARAKAAGVDAISGATPPYGKPDQNWGMGQRPAISMSWHAAETYCRWLTKVT
                     GKNYRLPTEAEWEYACRAGTTTPYFFPGDPAKFEKRGLFGGKPDTSVINTYVIYRGNS
                     PSKTREPSAVKPNPWGLRNMSGNVAEFCSDIYEGTSEHVIRGGSFRDGAAGVRSAARD
                     HTRTDEWLKTDPQIPKSIWWYSDCFHVGFRVVLTYSQQFVTPATEESQSPARGAGTAT
                     GSQQQLNRP"
     gene            1516..2847
                     /locus_tag="PLA17_08040"
     CDS             1516..2847
                     /locus_tag="PLA17_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK85403.1"
                     /translation="MMKKPSISRRNFLGTAAAAGAAGIVVPAFLTSCSTEKRAKVVLP
                     VMLDEAPDGPLLKAGLIGCGGRGTGAAINFLHAGPNLTIAAMGDVFADRIASCRERLA
                     KEGVEIADENCFTGFDAYEKVIDSGVDIIILATPPYFRPIHFEAAVSAGKHVFMEKPV
                     AVDPAGARQIMATGEKARGMDLCVVTGTQRHHQHDYVATWGKVQEGMIGEIVAANCYW
                     NQNQLWYRDPNPSWSEMEYMIRDWVNWCWLSGDHIVEQHVHNIDVINWFTGKKPVSAV
                     SFGARHRRVTGDQYDFFSTDFIYEGGIHVHSMCRQINGCEGNVSEWIQGTKGTSNCQN
                     TIWDPAGAELWKYDGYKLDDEGKMTDQLIRSPYDQEHIDLVTAIRTGNPLNEAESIAV
                     STMAGIMGRISAYTGKKVTWDEVMNSDLRLGPKVFDMGPVDVPKIVPVPGE"
     gene            2916..3854
                     /locus_tag="PLA17_08045"
     CDS             2916..3854
                     /locus_tag="PLA17_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPK85404.1"
                     /translation="MKSRSRRSFLRGTAATGAAFLSAPVILTGRSRTEYIPDADRPFK
                     LAYAPSLGMFRELCGSDAPLDNIQFIADKGFRALFDNGFPGRPEKEQEQIADKVQKTG
                     LLMGPWVLHADFGNTSFVLPHREVRDFLTAKMKEGIEVARRSGFRMALVVPGRYDYRI
                     HPDIQLANVIENLKYCADLVGPEGMTLVLEPLNTLRDHPGLWLTKMPQAYQVCKAVNS
                     PAVKIVDDIYHQQISEGNLIPNIETCWDEISAFHVGDNPGRNEPTTGEINYKNIFSYL
                     LSRDYKGVICMEHGRSIKGVEGARRVIEAYRECDPV"
     gene            3925..5103
                     /locus_tag="PLA17_08050"
     CDS             3925..5103
                     /locus_tag="PLA17_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPK85405.1"
                     /translation="MKLSKIPLLGRVVIAIILGIIIGQFAPVWFARIFATFNSLFGNF
                     LSFIVPLLIIGLITPAIGELGQGAGKLLAITALIAYISTLFSGFFTFFSCQAVFPHIL
                     STGIDTGALNGVEEIKIDPFFTIEMPPVMDVMTALILSFCIGLGITFLKGDILQKAFV
                     EFREIITLIIKSIIIPFLPLYIFGMFLSMSVSGQVYSVIVLFLKVILVIFILHILLLI
                     IQFLIAGAISGRNPMKALKGMLPAYATALGTASSAATIPVTLQCAINNKITPAIASFT
                     IPLCATIHLAGSAMKVTGFALAIQYFFGVPLDMVTFIGFIFMLGIIMIAAPGVPGGAI
                     MAAIGILEGILGFNEQMIGLMITLYIAIDSFGTACNVTGDGAIALIIDKISKKKPAAA
                     "
     gene            5200..6435
                     /locus_tag="PLA17_08055"
     CDS             5200..6435
                     /locus_tag="PLA17_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HPK85406.1"
                     /translation="MRIFRLVALAVFTVIIFSACSKLRSERDKPYVVMVSLDAFRWDY
                     DSIYGTPVLDDIARKGVTATRLIPSFPTKTFPNHYTIATGLYPDHHGLVNNSFYAPDL
                     DLVYRIGDRAMVSNGAFYGGEPVWVTARKQGMKSASFYWVGSEAPVQGIQPDYWKPYD
                     DEVPFGDRVDTVLKWLSMPINKRPHLVTLYFEEPDAVSHGYGPVSPETAAMVRSLDSL
                     IGVLRTGLGKLPNAADINLIVLSDHGMTEVDNSRYNYIFDTLPQRMVKRIYGGNPVWA
                     IEPAEGKSDSLLILLNVQRGMKAWKRNELPAHLHYGTHLRIPEILLVADPGWTAGLRP
                     EPRGDTRGDHGYDWRCRDMHSIFYAEGPAFKKGFSVDTLYNVDIYNIVTGVLGLVPAP
                     NDGNPERIAPLFNRRRSPL"
     gene            6987..7583
                     /locus_tag="PLA17_08060"
     CDS             6987..7583
                     /locus_tag="PLA17_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPK85407.1"
                     /translation="MTVRKDKYAAKAMNVFRQYGLHISIDEIAEKSGVTKKTLYNHFE
                     SKEGLLKYCLRTFVHDLEKKIAIMYSEEVNAIEGFISGVYGMGEVFHSLSPVFFDDLR
                     KMFPELSGTQHKAGFGSFLEGLKKNLEKGIMEKLYRQDINVELISRFFINSVVSFFIN
                     SVLKRSEYSARDYFRSVIDYHLHAVVTPKGRRLLEREK"
BASE COUNT         1995 a         1834 c         2041 g         1895 t
ORIGIN      
        1 agggtgttca ctacgccacc agatcgggct accatacagg ctgcgctgaa tgccaccttc
       61 caccgaaggg tgagggatac ctggttgaaa agacgaagac aggcttgcgt gatctgtgga
      121 gccactggac aaaggattct gccgattttg actgggaggc ggcatctgca cttgagagag
      181 cctcgaagca tgtttataat tccagctgca tttattgcca tgaaaacctt ttcccggcac
      241 agctgacaac agagggatca gaagctcatc tctactacaa gcagatgact gaaaagggag
      301 aggacctgca gtgcataagc tgccacctca atgccggaca ttatatagag ggttatttcc
      361 atgggagcaa caccggtttc ggtgcttctg tgaccagggt ggatacgctc tatcagtacc
      421 cggccactgt gacaggcttt gaagatttta ccgagcagat acctggctcg gcggtcacct
      481 tcaggatgat tgcggtgccg ggaggagagt tcatcatggg cagtcccggg ggtgagccgc
      541 tgcgcggcga agatgaagct gagaagaagg ttaaggttga ttccctgttc attgccgagg
      601 tagaggtgac ctgggatgag tacatggagt tttacaggca gacggcatcg gaagggagga
      661 gtaccgatac ggaaggtgca agggcaaagg ctgccggcgt tgacgccatc agcggagcca
      721 cccctcccta tggcaagccc gaccagaact gggggatggg acagcggccg gccatctcga
      781 tgagctggca tgccgcagag acctactgca ggtggctcac gaaggtcacc ggtaaaaact
      841 acaggctgcc gaccgaggct gagtgggagt atgcatgccg tgccgggaca acaacaccat
      901 acttcttccc gggagaccct gcgaagtttg aaaagagagg ccttttcgga ggcaaacccg
      961 atacttcggt gataaacacc tatgtgatct accggggcaa cagtccgtca aaaacccggg
     1021 agccgtcggc tgtgaagccc aacccctggg ggttaaggaa catgagcggc aacgttgctg
     1081 agttctgttc agatatctac gagggaacca gtgagcatgt cataaggggc ggatcattcc
     1141 gtgacggggc tgcaggtgtc cgcagcgctg cccgtgatca tacccgtacc gatgagtggc
     1201 tgaagaccga tccgcagata ccgaagagca tctggtggta ctctgactgt tttcatgtgg
     1261 gattccgggt cgtgcttacc tacagtcagc agtttgtgac tccggcaaca gaggagagcc
     1321 agtcccctgc acggggtgcc gggacagcaa ccggcagtca gcaacagtta aacagaccat
     1381 aatcataaag tattatcttt gactatccaa ccagattcac accgtgaaac ccgcatgcat
     1441 caagtacaca aacaattgtt tgaataatct acaatcatac gagcaccata atagagttaa
     1501 aatcaaaact atttaatgat gaaaaaacca tccatatcca gaagaaactt tcttggcact
     1561 gctgcagctg cgggagcagc aggcatcgtt gtgccggcat tccttacatc atgttcaaca
     1621 gaaaagcgcg ctaaggtcgt tctgccggta atgctcgatg aagcacctga cggtccgctt
     1681 ctcaaggcag gcctgatagg ctgcggcgga agaggcacgg gcgctgctat taacttttta
     1741 catgcgggac caaacctgac aatcgctgcc atgggtgatg tctttgctga caggatcgcc
     1801 tcatgccgcg aacgtctggc caaagagggc gtggagattg ctgatgaaaa ttgctttaca
     1861 ggtttcgatg cctacgagaa ggtaattgat tcaggggttg acatcataat acttgccaca
     1921 ccgccttact tccgcccgat ccattttgag gcagcagtct cggcaggcaa gcatgtgttc
     1981 atggagaaac ctgttgccgt tgatccggcg ggagcaagac agatcatggc cactggcgaa
     2041 aaggccaggg gaatggacct ctgcgttgtt acggggaccc agcgtcatca ccagcatgat
     2101 tatgtggcaa catggggcaa ggtacaggag gggatgatag gagagatcgt ggctgccaac
     2161 tgttactgga accagaatca gctctggtac cgtgacccca atccctcatg gtcagagatg
     2221 gaatacatga tccgtgactg ggtcaactgg tgctggcttt caggtgatca tatcgtggag
     2281 cagcatgtcc acaacataga cgtcatcaac tggttcacag gcaagaaacc ggtttcggcc
     2341 gtctccttcg gcgcacgcca ccgtcgcgtc acaggtgacc agtatgactt cttcagcact
     2401 gatttcatct atgaaggagg tattcatgta cacagcatgt gccgccagat aaacggctgc
     2461 gagggtaacg tctcggagtg gatacaggga acgaagggta cctcaaactg ccagaacacc
     2521 atatgggatc cggcaggcgc cgaactgtgg aagtatgacg gatacaagct tgacgatgag
     2581 ggcaagatga ctgaccagct gatccgcagt ccctatgacc aggagcacat cgaccttgtc
     2641 acagccatcc gtaccgggaa tccgctgaat gaagccgaat ccatagctgt ctcaaccatg
     2701 gccggaatca tgggaaggat ttcggcatat accgggaaga aggtcacatg ggatgaggtg
     2761 atgaactctg atctcaggct cgggccgaag gtatttgaca tgggcccggt ggatgtgccg
     2821 aagatagtgc cggtacctgg cgaataaaca atggatacct gcctgagtgc ctgcgggcac
     2881 tcaggctccg ggtaaacata ttaacagcat aatcaatgaa atcaagatca agacgcagtt
     2941 ttctgagggg taccgcagca accggcgcgg catttttatc cgctcctgtg attctgacag
     3001 gaaggagccg cacggaatat attcctgacg cagacaggcc attcaaactg gcttatgccc
     3061 cttcattggg catgttccgc gaactatgcg gaagcgatgc cccccttgac aatatccagt
     3121 tcattgccga caagggattc agggctctct ttgataacgg ttttcccggt cgtccggaga
     3181 aggagcagga acagatagcc gacaaagttc agaagacggg tctgctgatg gggccctggg
     3241 tgctgcatgc cgacttcggg aacacatcgt ttgtgctgcc tcaccgggag gtgcgcgact
     3301 ttcttactgc caaaatgaaa gagggcatag aggtggcaag gcgttcaggc ttcaggatgg
     3361 ccctggttgt tcccggcagg tatgactaca ggatccatcc tgacatccag ctggcaaatg
     3421 tcatcgagaa cctgaaatac tgtgctgacc ttgtcgggcc tgagggcatg acacttgtgc
     3481 tggagcctct gaacacactt cgtgatcatc ccggactgtg gcttacgaag atgccgcagg
     3541 cataccaggt atgcaaggcg gtaaacagcc cggctgtcaa gatagttgat gacatctatc
     3601 accagcagat cagcgagggg aatcttatac ccaacatcga aacctgctgg gacgagatat
     3661 cagccttcca tgtgggagat aacccgggac gcaatgagcc aaccaccggt gagattaact
     3721 acaagaacat cttctcctat ctgctctcgc gtgactataa aggggtgata tgcatggagc
     3781 atgggcgcag cataaaaggg gttgagggag cacgccgggt tatagaggct taccgtgaat
     3841 gtgatcctgt ctgattgatg gtgccgtgat agttgcttgt ccataaattc gtagctttag
     3901 cgaaattatt ttacctgatt tattatgaaa ctctccaaaa tcccccttct tggcagggtg
     3961 gtaatcgcga ttattctcgg aattattatt ggtcagttcg ccccggtctg gtttgcccgg
     4021 atttttgcaa ctttcaactc gctgttcgga aattttctct cttttattgt tccgctgctc
     4081 atcataggtc ttatcacgcc tgcaattggt gagctgggac agggagccgg gaaattgctg
     4141 gccattaccg cgctcatagc gtatatttcc accctattct cgggcttctt cacttttttc
     4201 tcatgccagg cggtatttcc ccatatactc tccacaggta tcgataccgg agcgctgaat
     4261 ggagtagagg agattaagat tgatccgttc tttaccatcg aaatgccgcc ggtaatggat
     4321 gtgatgacag cactgattct ctctttctgc atcggtctgg gcatcacatt cctgaaaggt
     4381 gatatcctgc agaaagcatt tgtggaattc agggaaataa tcaccttgat cattaaaagc
     4441 attatcattc cgtttcttcc gctctacatt ttcggaatgt ttctttccat gtcagtttcg
     4501 ggccaggttt attcggtaat agtgctgttc ctgaaggtta tactggtaat cttcattctt
     4561 cacatattgc tgcttataat acagttcctg attgcgggag caatatcagg gcggaacccc
     4621 atgaaagcgc tcaaagggat gttaccggcc tatgcaaccg cactgggaac ggcatcctct
     4681 gcagctacta tccctgtcac tcttcagtgt gcaataaaca ataaaatcac tccggcaatc
     4741 gcctccttca ctatacccct gtgtgccaca atacacctgg ccgggagcgc catgaaggtt
     4801 acgggattcg ccctggcgat tcaatatttc ttcggggtac cactggatat ggtcactttc
     4861 atcggcttta ttttcatgct ggggattata atgattgctg cacccggtgt gccggggggc
     4921 gccattatgg cggcaatcgg gattcttgag ggcatacttg gcttcaatga gcagatgata
     4981 ggactgatga tcacacttta cattgcgatt gacagtttcg gaacggcatg caatgtgacg
     5041 ggtgatggtg caatagccct catcattgac aaaatatcaa aaaagaaacc agcagctgcc
     5101 tgaccggact ggcagtgaca atcagaagag cggtagccac ggagccagaa agattaaaca
     5161 ttgttattcc ttcagaaaca aaaacggttt gcagaacgga tgaggatttt cagattagtt
     5221 gcattggcgg tttttaccgt aattatattt tcagcatgca gtaaactcag gagtgagaga
     5281 gacaaaccat acgtcgtaat ggtctccctg gatgccttca ggtgggatta tgactcgatt
     5341 tatggcactc cggtgcttga tgacatagcc cgtaaagggg tgacggccac caggctgata
     5401 ccgtcgtttc cgacaaaaac ctttccgaac cactatacaa ttgccaccgg actataccct
     5461 gatcatcacg gactggtcaa caacagtttt tatgcacctg accttgatct tgtttacagg
     5521 ataggtgacc gggccatggt ctccaacgga gccttttacg gtggcgaacc ggtttgggtt
     5581 acagcccgga aacaggggat gaagtctgcc tcgttctatt gggtggggtc ggaggcgccg
     5641 gtacagggga ttcagcctga ttactggaaa ccgtatgatg atgaggtgcc gttcggtgac
     5701 cgcgttgaca ctgtgctgaa gtggctctcc atgcccataa ataaaaggcc gcaccttgta
     5761 accctctatt ttgaggagcc cgatgcggta agtcacggtt acggccctgt ttctccggaa
     5821 acggctgcaa tggtaaggag cctcgacagc ctcattggtg ttctccgcac cggtctggga
     5881 aaactgccca atgcagccga tatcaacctc attgtccttt ccgatcacgg aatgacggag
     5941 gttgacaatt cgcgctacaa ctacatcttt gatacccttc cgcagagaat ggtgaagagg
     6001 atttatggcg gcaatcctgt ctgggccata gagccggccg aagggaagtc agacagtctg
     6061 ctcatcctgc taaatgttca gcgcgggatg aaagcctgga aaaggaatga gttgccggca
     6121 catctgcatt acggaacaca cctgcgaata ccggagatac tccttgttgc cgatcccggc
     6181 tggaccgcag gtttgaggcc tgaaccacgg ggtgacacca ggggtgatca tggttatgac
     6241 tggaggtgcc gcgacatgca ctcgatcttc tacgccgagg gacctgcctt taagaaaggt
     6301 ttcagcgttg acacgctgta taatgttgat atatataata ttgtgacagg tgtccttggt
     6361 ctggttccgg cccccaatga cggcaacccg gagcggatag ccccgctgtt caatcgacga
     6421 aggtctcctt tatgatggct ttgacctcgt tattacattg ctgcaagatt tccatattta
     6481 tttacaatag tccggtatac tctcaaatga ttgaccataa cggccttatt gtctgacttg
     6541 atcacatccc ttgaaatgaa cagcgtaatt aatagattat gtggctataa attgcctggc
     6601 cgttaattca gggagtacct gatctgaatg ttataggtct caaatattaa acagtaatct
     6661 caattccgga tagaaatggg ttgtgggtca tctgcccggg ttccggacag ccagtctgat
     6721 cctttcagcc cctgctgttc aacttcccgt attccgggta gccagcctga tactttcagc
     6781 ccctgtggtt catcttcccg ggttccgggt agccagcctg atcctgccgg ttaactgacc
     6841 ggatagtgtt atgaagttta taaaagctga acaactgatt gctcaggcca ttaatacctt
     6901 gtaaaattaa tatacaaatt acactatata aatataatta gtgtatattt gcaaccgtta
     6961 ttcaattaaa aacaatcagt gatccgatga ctgtcaggaa ggataaatat gcggcaaagg
     7021 ccatgaatgt cttcaggcag tatggccttc atatttcgat agatgagatt gctgaaaaat
     7081 caggtgtcac gaaaaagacc ctctataatc attttgaatc gaaggaaggt ctgctgaaat
     7141 actgtctccg gacttttgtt catgaccttg aaaagaagat agccataatg tattctgaag
     7201 aggtcaatgc aattgaagga ttcatatcgg gtgtttacgg aatgggcgag gtctttcatt
     7261 cactgagccc ggtctttttt gatgacctga gaaagatgtt cccggaactc tcgggtacac
     7321 agcataaagc cggattcggc tcttttctcg aaggattgaa aaaaaatctt gaaaagggga
     7381 taatggaaaa gctctacagg caggatataa atgttgaatt gatcagccgg tttttcataa
     7441 attcagttgt cagttttttt atcaacagtg ttctgaaacg cagtgagtat tcagccaggg
     7501 actatttcag atcagtaata gactaccacc ttcatgcggt tgtcaccccg aaggggcgaa
     7561 gattgcttga aagggaaaag tgaatcacga aatattctga gaaaaatgaa tagagccaga
     7621 actattttat ttacaggaat aatctgtata tgtgccacta tcagtgctca gcaggtttac
     7681 gatctccagg gttgcctgaa gtatgcactt gagaacaacc ataatatcag gaaggcacag
     7741 ttcgacaaag agaaatcggc tcagg
//
LOCUS       DAOPBK010000128         7626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8718_length_7626_cov_5.943997, whole genome shotgun sequence.
ACCESSION   DAOPBK010000128 DAOPBK010000000
VERSION     DAOPBK010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8718_length_7626_cov_5.943997"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PLA17_08065"
     CDS             <1..1113
                     /locus_tag="PLA17_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPK85408.1"
                     /translation="KMYDQYHPLGIVGIVTSFNFPMAVWAWNTMLAVTGGNVVVWKPS
                     SRTPLTAVAVHKVISGVLSANEVPEGVFNLVVAKSSVLGDRFIEDRRIALLSITGSTR
                     VGRHAAQLAGKRFGKVILELGGNNAALVMPSANLDLAIPSIVFGAVGTAGQRCTTTRR
                     VIVHEAIYDKVKERLVNAYRQLESRIGNPLNENTLIGPLIDSAAVDAYKIAISNALSE
                     GGKLAYGNSIVSGPGIETNNFVMPTIIEAQNHFRIVQEETFAPILYLIKAGSLEEAIA
                     FNNDVPQGLSSTLFTTSLAEAEQWLAEGGSDCGIANVNLSTSGAEIGGAFGGEKDTGG
                     GRESGSDAWKLYMRRQTVNVNYGNEMPLAQGIRFEL"
     gene            1144..1539
                     /locus_tag="PLA17_08070"
     CDS             1144..1539
                     /locus_tag="PLA17_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPK85409.1"
                     /translation="MTAQQDLTGPVLSVRGRFWINIEGVSRLGKGKIELLEKIRDAGT
                     LLKAAAELNIPYRQAWSKVQSMNKLQDYPVVVLRHGGKDRGSARVTEFGNNLINAFRI
                     IESNLEKFLEDQASILEFPREETPEDQGR"
     gene            1600..3330
                     /locus_tag="PLA17_08075"
     CDS             1600..3330
                     /locus_tag="PLA17_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPK85410.1"
                     /translation="MSNRRLSEKWIKASIIGTIWAASEIVLGSFLHNLRVPFSSNFLT
                     GIGIIIMISTSYKWKERGLFWRAGLICALMKTMSPSAVIFGPMVAIFSQSVMLELFTR
                     VFGRNAVGYLTGAIFAMTWNLFQKIMNFIIYYGFNIANIYADLLKYAERQLNIDFDLV
                     WSPIFALAAIYATLGVIAGIAGMMVGRRLVREPAAYRLGHARKNNNRLSPDRQQFNYS
                     VSWLFLNIVLIITAFFLLNYAGWPVWSIAITAIVIVWILRYKRALRQLSRPRIWIFFV
                     IITMATSFVIGKIQSDDWIRGLEIGIQMNFRAMIVILGFSVMGTELYNRKVREFFART
                     AFRQLPFALEISMKSLPMTIASVPEAKVIARSPVSVICSVISQIEQRLTEVKKELSRH
                     IYIITGAIGEGKTTQVRKLVEQLKAGNVSVMGIYSPRIMVDGQTAGYDVVDVDSGLRV
                     PFLRVDSKSESKKIGRYSINPAAIEAGLKSLSAAMDSNPDVIVADEIGKMELGNEGWF
                     EKIDGMLKGSSILVFAVRDSFVEEIVNKFEMKDYSVMPVSEHLHLELARVIEERIASY
                     RPAQQSARDY"
     gene            complement(3400..3636)
                     /locus_tag="PLA17_08080"
     CDS             complement(3400..3636)
                     /locus_tag="PLA17_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85411.1"
                     /translation="MPGNRIMTPEPVIGSEGRTNSSMLRKRLAHAPRIRITYGNTGPL
                     QAGGKAKDQHPELIVVPAATWRCAPLSPGKEKIQ"
     gene            3805..4413
                     /locus_tag="PLA17_08085"
     CDS             3805..4413
                     /locus_tag="PLA17_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85412.1"
                     /translation="MKKNRIINGLLISAFFFSVTLASAQTRMPDELNTAAIREQIEYV
                     EDHTRIYDNFRAIREDIFQKLNKNILDSLTAVKNKVAELKGDTANLISRTDSLNVLLT
                     STRSQLEEVTATKNKIRVFGMEINKTTYNTIMWILVGGLILLMVLGFLVFKRNLVVLL
                     RTEKDLKELKEEFATYRQTSRIAREKVEMDLFRANQKLKGLV"
     gene            4837..6099
                     /locus_tag="PLA17_08090"
     CDS             4837..6099
                     /locus_tag="PLA17_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85413.1"
                     /translation="MRKTILTCLIIMGSASAFGQVVADTLRKDALKVFMEASDYIRKE
                     IPWVNYVRDIKDAGLYIISTSQNTGSGGVEYTYFFIGQKENEGMRDTLRFTSSPDETA
                     EIIRENEVKTLKMGLVRYIAKTPLSKYMSIGFSEPLSETVSTDKWNSWVFKTSLSGYA
                     SGEKSYRSSYLNGSFSANRVTEKLKSSVSLKYSYSTNKYDVNDEVISSEYNSKGLSGL
                     LVGSLTDHWSVGGSMYAGASKYNNTDLSLNIQPGIEYNVFPYSESTRRQLRLTYKAGF
                     SYENYIDTTIYNKVKENLLSHSLTISYEVIQKWGSADVSLDYSNYLHDWSKNNLSLNA
                     FLNLRITKGLSLNFGGGGALVHDQLGLVKGGATQQEVLLRLRELETQFEYFTTFGLTY
                     TFGSIYNNVVNPRFGNTGGSGGMTIIMH"
     gene            6426..>7626
                     /locus_tag="PLA17_08095"
     CDS             6426..>7626
                     /locus_tag="PLA17_08095"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK85414.1"
                     /translation="MKNLLKASLILLFVIAVSCQRKAADELSLPFEKYELENGLNVIL
                     HQDHSDPIVSVAIYYHVGSSREVPGRTGFAHLFEHMMFQQSENVPEDQYFSFIQSAGG
                     TLNGSTNQDRTNYFETVPKNALEMVLWMESDRMGYLTNTITQQAFAIQQNVVQNEKRQ
                     NYDNRPYGHSSTVMAKALFPEGHPYSWTTIGEMKDLFNATVDDVKEFHARFYVPNNAT
                     LSIAGDFDAAEVKALVQKYFGEIPAGADVEKMSPMPVTLAETKKLYHEDNFANTAQYT
                     MAFPTVEMYNPDSYALTILSNILAGGKKSPMYNVLVKEKMLTSGVSAYNQAQLLAGTF
                     RISVNANPGVSLADIENAIFESFERFEAEGFTEKDLEKVKAGQETYFYNRISSVDGKS
                     KQLAEYNM"
BASE COUNT         2098 a         1655 c         1931 g         1942 t
ORIGIN      
        1 aagatgtacg accagtatca tccgcttggc atagttggca ttgtaacatc attcaatttc
       61 ccgatggcag tgtgggcgtg gaacacaatg ctggcagtga ctggaggcaa tgtcgtggtc
      121 tggaaaccaa gctccaggac acctcttacg gccgtggcag tgcataaagt cattagcggg
      181 gtcctttctg caaacgaggt gcctgagggt gtttttaacc ttgtagttgc aaagagctca
      241 gtacttggtg acaggtttat tgaagacagg cgtatcgctc tcctgtcaat taccggatca
      301 acgagggtgg gaaggcatgc tgctcagctg gccggcaagc ggtttggcaa ggtaatactt
      361 gaactgggag gcaacaatgc tgcattggtg atgccttcgg caaaccttga tctggcaatc
      421 ccgtcaattg tgtttggtgc tgttggtacg gcaggtcaga gatgcacaac caccaggagg
      481 gttattgtgc atgaagcaat ttatgataag gtaaaggaga ggctggtcaa cgcatacagg
      541 caactggaaa gccgtatcgg aaatccgctg aatgagaata ccctgattgg cccgctcatt
      601 gattcggcag ccgtggatgc gtacaaaata gcaatcagca atgcattgag cgaaggaggc
      661 aaactcgctt acggaaacag catagtttcc ggtcccggga tagagacaaa caactttgtt
      721 atgccaacaa ttatcgaggc tcaaaaccat ttcagaatag tacaggaaga gacttttgct
      781 ccgattctgt acctcattaa ggcaggttct cttgaagagg caatagcatt caacaatgat
      841 gtgccgcagg gcctctcttc gactctgttt acaaccagcc tggctgaggc agaacaatgg
      901 ctggcagaag gcggatcaga ctgcggtata gcaaatgtca acctgagcac atccggcgcc
      961 gaaattggcg gggcctttgg aggagagaaa gataccggcg gagggcgcga atcgggttct
     1021 gatgcgtgga agctgtacat gcgccggcag actgtcaatg taaactacgg taacgagatg
     1081 cctctggccc aggggattcg ttttgagctt taatgtgact ggagatataa aacactttat
     1141 accatgactg cacagcaaga cctgaccgga cctgttttgt cggttcgcgg gagattctgg
     1201 ataaacattg aaggagtctc ccggctggga aaagggaaga tagagcttct tgagaagata
     1261 agggatgcag gcacactgct caaagctgcc gccgagctta atattcctta ccggcaggca
     1321 tggagcaaag tgcagagcat gaataagctt caggattatc cggtggttgt tctcagacat
     1381 ggaggtaagg accgcggaag tgcacgggtt accgaattcg gcaataatct catcaatgct
     1441 ttcaggatca tcgagtcaaa cctggagaag ttccttgagg accaggcatc aatattggaa
     1501 ttcccccggg aagagactcc ggaagatcag ggccgatgat acaaatgttt aaccagagat
     1561 ggataagcaa agacgaagca cgtaaaacct gccttataaa tgagtaaccg cagactcagc
     1621 gaaaagtgga tcaaagcctc cataattggt acgatctggg cagcatcaga gattgtactc
     1681 ggcagttttc ttcataacct gcgcgttccg ttcagttcaa actttcttac gggcatcggg
     1741 atcatcataa tgatctcgac aagctacaaa tggaaagagc ggggtttgtt ctggcgggca
     1801 ggactgatat gtgccttaat gaagaccatg tcacccagcg cggtcatctt cggccctatg
     1861 gtggcaatct tcagccagtc ggtaatgctt gaactgttca caagggtttt cggaagaaat
     1921 gcggtcgggt acctgacagg tgccatattc gcaatgacgt ggaacctgtt ccagaagata
     1981 atgaacttca ttatctatta cggattcaat atcgccaata tctatgctga cctgttaaaa
     2041 tatgcagaga ggcagctgaa tattgatttt gacctcgtct ggtcacccat ttttgccctt
     2101 gccgccattt atgccacact gggagttatt gccggtatag cagggatgat ggtcggtcgg
     2161 cgccttgtaa gggagcctgc ggcataccgg ctgggacatg caaggaaaaa caataataga
     2221 ctaagtcctg accgtcagca attcaattat tcagtttcgt ggctgttctt aaatatcgtg
     2281 ctgataataa ctgccttttt ccttctgaac tatgccggct ggccggtctg gagcattgca
     2341 ataacagcca ttgtcatcgt ctggatatta agatataaaa gggcgctgcg acagctttca
     2401 aggccccgta tctggatctt ttttgtgatt attacgatgg caacttcatt tgttattggc
     2461 aagattcagt ctgatgactg gataagagga ctggagattg gtatccagat gaacttcagg
     2521 gcaatgattg taatcctcgg gttttctgtt atgggaacag aactctataa ccggaaggta
     2581 agggagtttt ttgcaaggac agcattcaga cagttgccgt ttgcattgga gatatcgatg
     2641 aagagcctgc ctatgacgat tgcatcagtg ccggaggcaa aggtcattgc ccggagtccg
     2701 gtttccgtga tttgctcggt aatttctcag attgagcaga ggcttacgga ggtcaagaag
     2761 gagctttcca ggcatatcta tatcataacc ggtgccatcg gggaggggaa aacaacccag
     2821 gtgcgaaaac tggttgaaca gttaaaggcc ggcaatgtca gtgtaatggg tatttattct
     2881 ccaagaatca tggttgatgg ccagacggca ggttatgatg ttgtggatgt tgattcagga
     2941 ttaagagtgc cgtttttgcg tgttgacagt aagagtgaat caaagaagat aggccggtac
     3001 agcattaacc cggctgcaat tgaggccgga ctgaaatcac tttcagctgc catggacagc
     3061 aatcccgatg tgatagtggc agatgaaata ggaaagatgg agcttggcaa tgaaggatgg
     3121 tttgagaaga ttgatggaat gttgaagggc tcttcaatcc ttgtctttgc cgtgcgggat
     3181 tcctttgttg aggaaattgt taataagttt gaaatgaaag attattcggt catgcctgta
     3241 tctgaacatc ttcaccttga gcttgcccgg gtgatagagg agcgcatcgc cagttaccgg
     3301 ccggcacaac aatctgctcg ggattactga atcttctctt tcccggatga tagagtagcg
     3361 tatctccagg tagcggccgg cacaacaatc tgctcgggat tactgaatct tctctttccc
     3421 gggtgataga ggagcgcatc tccaggtagc ggccggcaca acaatcagct cagggtgctg
     3481 atccttcgct ttccccccgg cctgtagggg gccagtgttg ccataagtta tccttatcct
     3541 gggagcatga gcgagtctct tcctcagcat tgatgaattc gtccttccct ctgaacctat
     3601 tacgggctca ggggtcatta ttctgttccc gggcatggcc gcccccgtcc tgcggtatca
     3661 cagtcagccg ctcaggggtc atctgtctgt tctcaataac tgactttaaa acaatgaatc
     3721 ataatctttt aatgttacaa aatttttgta ttttccttgc gttaacaaat aaggcaatac
     3781 tgtgaaaaca ataaaccggg gcaaatgaaa aagaacagaa tcataaatgg actgctgatt
     3841 tccgcttttt tcttttcagt gacacttgca tctgcccaga ctaggatgcc tgatgaactt
     3901 aacaccgcag cgatcaggga acagattgag tatgtggagg atcataccag gatttatgat
     3961 aacttccggg ccataagaga agacattttc cagaagctca acaagaatat ccttgattca
     4021 cttacagccg tgaaaaacaa ggtggcagaa ctgaagggcg atactgcaaa tctgattagc
     4081 cgtaccgatt cgctcaacgt tttgctgaca tcaaccagga gccagcttga ggaagttacc
     4141 gccacaaaaa acaagatcag ggtattcggt atggagataa acaaaacaac ctataacacc
     4201 atcatgtgga tactggtggg cggattgata ctgctcatgg tgctgggctt cctggtcttt
     4261 aaaaggaacc ttgttgttct cctccgtacc gagaaagacc tgaaggagct caaggaggaa
     4321 tttgccacct acagacaaac gtcaagaata gcccgcgaga aagtggagat ggacctcttc
     4381 cgcgcaaacc agaagctcaa agggctggtc tgacagtatc gttttccggt gttcaggggc
     4441 tgatctgaca gcatcgtttt tcactattca ggggctgccc tgacagtctg gttttccggt
     4501 attctggcgg ggcatggctc ccctgaaggg tatggaactg ccgggactcc tcccgggcca
     4561 tcttgttttt cagcatcgag acatagagtt catgaggctt gtgacattgt cacggttgct
     4621 ttctgtcata tccgggaagc tggagatatg atctaattca tcatggaagc ggacggtggc
     4681 tttcacagaa gaggcttttg cgaaggctga ccagattgct gcgaaggtaa atggacaagt
     4741 caggggacag ttgcacaact accggcaaat actttacttt gggcaatttt ttatctgtac
     4801 cgctttatgt taaacaaaat caggaaaaga gacacaatga gaaaaacaat actgacatgc
     4861 cttatcatta tgggttctgc atctgctttc ggccaggtgg ttgccgacac actcagaaaa
     4921 gacgccctta aagttttcat ggaggcatct gattatatcc gcaaggaaat tccctgggtc
     4981 aattatgtac gggatataaa ggacgccggg ttatatatta tttcaacttc tcagaatacc
     5041 ggttcggggg gagtggagta tacctatttt ttcatagggc agaaggaaaa tgagggaatg
     5101 cgtgacacac tcaggtttac ttcgagtcct gacgagactg ctgaaatcat cagagagaac
     5161 gaggtcaaaa cattgaaaat gggccttgtc cgctatatag ccaaaacacc attgtcgaaa
     5221 tatatgtcga tcggtttcag tgagccgctt tctgaaaccg taagcactga caaatggaac
     5281 agctgggttt ttaaaacctc actttcaggt tatgcttcag gagagaagtc ataccggtca
     5341 tcatacctta acggcagttt ctcagccaac agagtgactg aaaagctgaa gtcaagtgtc
     5401 tctctgaagt acagctactc tacaaacaaa tacgatgtga atgatgaagt gatcagcagt
     5461 gaatataatt caaaaggttt aagcggactc ctggtgggca gccttactga tcactggtca
     5521 gtaggaggca gcatgtatgc cggcgcatca aaatacaata atacggatct ttccctgaac
     5581 atacagccgg ggatagaata caatgttttt ccatattcgg aatcaacgcg ccgccaattg
     5641 aggcttacct acaaggcagg attcagttat gagaattata ttgacaccac tatctacaac
     5701 aaggtaaagg aaaacctcct ctctcattcc ctcacgataa gctatgaggt tatccagaaa
     5761 tggggatctg cagatgtgtc tctcgattac agcaactacc tgcatgactg gtcaaaaaac
     5821 aacctctctc tcaatgcttt ccttaacctt agaataacga agggactctc ccttaatttc
     5881 ggaggtggcg gagcacttgt tcatgaccag ctgggtcttg taaaaggagg agccacacaa
     5941 caggaggttc tgctcaggtt aagagaactg gagacccagt tcgagtattt taccactttc
     6001 gggttgacat acactttcgg ctccatctac aataatgttg taaatccccg gttcgggaac
     6061 accggaggaa gcggagggat gactatcata atgcactgat cagcctttag ttctgtacct
     6121 aaggctgacc ggaaacatgc cttcccgggt cagcctggcg ggttcgttcg gagaggctga
     6181 attatctgcc tgcggggtgc acattgtcag tccggcagtc agaatgatgg cagagaagtg
     6241 cagaatacgg ttcatgacag gttccttatg atacccaaaa agaagtaaaa tggactgttg
     6301 cagcatcttt cgcgggttcc ctcaggggaa gaatgtgaat aaatgttaaa agaggccggt
     6361 tatgaagagg ataatctgta aattagctga atataattca atcagttaac ccaatctcca
     6421 aaatcatgaa gaacttattg aaagcatctt tgatcttgtt gtttgtcata gcagtctcct
     6481 gtcagaggaa ggcagctgat gaactatcac ttccgttcga aaaatacgaa ctggaaaatg
     6541 gtctgaatgt aattctgcac caggatcact cagatcccat tgtttcagtg gcaatttact
     6601 atcatgtagg ttccagccgt gaggttccgg gccgtaccgg ttttgcgcac ctgtttgagc
     6661 atatgatgtt ccagcagagt gaaaatgttc cggaagatca gtatttcagt tttattcagt
     6721 ctgccggtgg aacactgaac ggatcaacca accaggatcg caccaattat tttgaaaccg
     6781 tgcctaaaaa tgccctggaa atggtgctct ggatggagtc tgaccgcatg ggatacctga
     6841 caaacaccat tacacagcag gcttttgcca ttcagcagaa cgtggttcaa aatgagaaac
     6901 gccagaacta tgacaacagg ccttatggtc attcgtcaac ggtaatggct aaagcgttgt
     6961 ttcctgaagg tcatccctac agctggacga ccatcggcga gatgaaggat cttttcaacg
     7021 ccacagttga tgatgtgaag gagtttcacg cgcgttttta tgtaccgaac aatgcaaccc
     7081 tttcaattgc aggcgatttt gatgccgctg aagttaaagc attggttcag aagtattttg
     7141 gcgagatccc ggctggggcc gatgtggaaa aaatgtctcc catgccagta acccttgctg
     7201 agacgaaaaa actatatcac gaagacaact tcgccaatac tgcacaatac acgatggctt
     7261 ttccaacagt tgagatgtac aatcccgact cctatgcact gaccattctg agtaatatac
     7321 ttgccggtgg taaaaagtca ccgatgtaca atgttctcgt taaggagaag atgcttacct
     7381 ccggtgtgag tgcctataac caggcacagt tgcttgccgg cactttccgc atatcagtca
     7441 acgccaatcc cggtgtttct cttgcggata tcgaaaatgc catctttgaa tcctttgaac
     7501 gctttgaagc cgagggattt accgagaagg acctggaaaa agtcaaggca ggacaggaga
     7561 cctattttta caacaggatc agcagcgttg acggcaagtc gaaacagctt gctgaataca
     7621 acatgt
//
LOCUS       DAOPBK010000129         7469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_8938_length_7469_cov_4.747505, whole genome shotgun sequence.
ACCESSION   DAOPBK010000129 DAOPBK010000000
VERSION     DAOPBK010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8938_length_7469_cov_4.747505"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PLA17_08100"
     CDS             complement(<1..1096)
                     /locus_tag="PLA17_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85415.1"
                     /translation="MKRFFYIIPVILVIASACSSLRFTGAYYNDDLYYRPSENTVAVT
                     ASTVQGRNDGQAYYDNIYAADTLIADEYVPADEYSEYAAGGEQTIVYNYYGSAADRIY
                     LFSDDYFYPYWRDPFYYSPFSLRLSLGYGYWGPYGSFYSWGYDPFYYDYYYPYSYSYY
                     YPYSYYSPYYSWGYNPYSYYNYYGGYYGYGYYGYYPWYSGYGYPSHYNDGGYVQNIAR
                     RGGYSTMSRGSYSNTDTRTKSGYAPSGDINSRRIASGTALPAGTGDTPAGSRRTATET
                     TAGRNAAVAGTTTGARRTQDGTVSNAGQGTETVSKPEYARRESGNTMVQNQTRTQTQT
                     PVRSETTTQTRTQQQAPVRTQTPVRITQTPV"
     gene            1296..2771
                     /gene="proS"
                     /locus_tag="PLA17_08105"
     CDS             1296..2771
                     /gene="proS"
                     /locus_tag="PLA17_08105"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPK85416.1"
                     /translation="MAKFLTNRDENYSRWYNDLVIKADMAENSAVRGCMVIKPYGYAI
                     WEKIQSELDRMFKATGHVNAYFPLFIPKSFFSREAAHVEGFAKECAVVTHYRLKNSPD
                     GKGIVVDPDARLEEELIVRPTSETIIWNTYKDWIQSYRDLPLLINQWANVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATREEAEEEAIKILNLYTDFVENYMGVPVVRGVKTESEKFA
                     GAIDTYTIEMLMQDGKALQGGTSHFLGQNFAKAFDVQFTNQEGKLDYVWATSWGVTTR
                     LIGALIMAHADNNGLVLPPKLAPIQVVIVPIYKGNDQLDKISDIAVDIRQQLEKAGFT
                     VKYDNRDTHKPGFKFAEYELKGVPVRLAIGPRDAEDGTVEVARRDTLTKETIRRENIV
                     THVTSLMEEIQQNIFRKAVEFRKENTFTADTWEEFTDIIENRGGFVLAHWDGTAESEE
                     KIKELTKATIRAIPFDSREEKGKCILTGNPSNRRVLFARAY"
     gene            2904..3617
                     /locus_tag="PLA17_08110"
     CDS             2904..3617
                     /locus_tag="PLA17_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85417.1"
                     /translation="MADEITQSKVLIVDTLKGKSVLKQKVFDNTATAFLMIKDILKNL
                     ARDVNSTLSGVDQRIRMEYTDRSTFDAQLKVAADLLMFSMHSNIFQFDREHPAWKTAY
                     IQKNRFNSYSGIINIYNFLADSFKYSRLDDLGYLIARIFINHENQCFVEGKRQMGMLF
                     TNYGNEAFSQESLKKIVYTAINYSLEFDLLVPPYDTVKIASVGQAESKIQHSRLITGK
                     RLGFQFNSDDILDDNSKPR"
     gene            3614..5437
                     /locus_tag="PLA17_08115"
     CDS             3614..5437
                     /locus_tag="PLA17_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85418.1"
                     /translation="MNPFTNRSITTLFFFFLSLFFTLPGRPVAAQDTTVPDTLSRAFY
                     DSLRVRAEKRRLTRLLYDMIIVTPAPSGHAREELASTTAYEAYEGRKIRNIEIIRLDA
                     FGQNIDNPEGKTPSRAQRFMNSTYTKTRRFVLSQNLVFKTGDTISSLELSDNERLLRR
                     LPFIDDSRITVVPADSNQADIAVIVREKYPVGANLRIDDIRSGRVGIFTSNFVGLGHE
                     LEISTPYDFNEYDAPGIGLRYSINNIARTFSRLNMHFSDGLGTTHMGGAFSRNFATSE
                     TRWAWSASVTFTRTNDRIDTMAVPAPLRFTWQDYWAARSFMLDRSTVTRLIITGRYMH
                     NNVFRRPGIDAFSYYRMQNYQSVTGSIALSSQRFLNTSLIYSYGRTEDIPYGYMAELL
                     LGREKNEFKWRSYAGGTIAYGNFYTGVGYLYTGAGISAFYNNGHTEQGLFQGSLRYFT
                     PLARVGRSRMRTFINLNFCRGFNRYTDEYLYFRDDGLIRGFRNDSLGGNTRIAVSVEP
                     VLFVYKPVVGFRFALFAFADAGLLSRGRQMGSDYYTVAALGGGVRIRNDQLVLNTLQL
                     RIAWYPNSPPWSESSLLTANSIMRLRPPGFEPDPPGLVPYL"
     gene            5534..6850
                     /gene="tilS"
                     /locus_tag="PLA17_08120"
     CDS             5534..6850
                     /gene="tilS"
                     /locus_tag="PLA17_08120"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPK85419.1"
                     /translation="MLSRFLEFSEREKLFTGAGRILLAVSGGIDSMVMAWLFREAGIE
                     HSVAHCNFSLRGDESDGDEEFVASYARINNIPFFSKRFDTLVYAGTNRISVQMAAREL
                     RYNWFRSLLCSEGFDAVAVAHNLNDNVETFLINLSRGTGLQGLTGMSPRTGEIIRPLL
                     FATRDDIVSCATARGIDFREDRSNAELKYIRNRIRHKVIPELGKVNPGILSAVVDTMK
                     NLASASALMDTYRYQLGRQLFTSSGDTTVVEISSLLELNPLAPHIFELFRPFGLFPKQ
                     TDEVISLLQAGTGKMVYTATHRLLNDRGRIIISPGKPEAPSEYRFNSIDEMRLSGLFS
                     AVRITEPSEDALPATRLTASLDLDLVTFPVTVRQWKPGDRFMPLGMRQMKKISDFLID
                     MKVPVTEKEQVMLLLSGGDVMWVMSYRIDNRFRVTEETGKILVLTL"
     gene            complement(6962..>7469)
                     /locus_tag="PLA17_08125"
     CDS             complement(6962..>7469)
                     /locus_tag="PLA17_08125"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPK85420.1"
                     /translation="AIAKAMETNDTIGGVVECVVKNVPKGLGEPSFMSVESLLGMIAF
                     GIPAVNGVEFGAGFASSRSYGSLHNDPFIDARGKTSTNNAGGINGGITNGNDLIVRVS
                     VKPAASTGVTQKTFDTETGEMTDLEITGRYDTCIARRIPVILESAVAIGLADLMMIDR
                     GINGIRER"
BASE COUNT         1895 a         1806 c         1903 g         1865 t
ORIGIN      
        1 tgactggtgt ttgggttatt ctgaccggtg tctgtgttct gaccggtgcc tgctgctgtg
       61 ttctggtctg ggtggttgtt tcactcctca caggagtctg agtctgggta cgcgtctggt
      121 tctggaccat tgtattaccc gattcgcgtc tagcatattc aggttttgag actgtttcag
      181 tgccctgacc ggcattgctg acagttccat cctgggttct gcgggcaccc gtggttgttc
      241 cggcaactgc ggcattcctg ccggcggtgg tctcagtggc tgtcctcctt gaacctgcgg
      301 gagtgtcacc tgtaccggca ggcaatgcgg tgccggatgc gatcctcctg ctattgatgt
      361 cacctgaagg tgcatagcct gacttggtcc ttgtgtcagt gttggagtat gagcctcttg
      421 acattgtgct gtatcctccc ctgcgggcga tattctgcac atatccgccg tcgttgtaat
      481 gtgagggata tccgtagccg gagtaccagg gatagtatcc gtagtatccg taaccataat
      541 agcctccgta gtaattatag taggaatacg ggttgtatcc ccatgaatag tacggagagt
      601 agtatgaata gggataatag tatgaatagg aatagggata gtaatagtca tagtagaagg
      661 gatcatagcc ccatgaatag aatgatccgt aaggccccca gtagccataa cccaggctga
      721 gtctcaaaga gaacggtgag tagtagaacg gatctctcca gtaaggatag aagtaatcat
      781 cactgaagag gtatatcctg tcagccgcgc tgccgtaata gttgtatact attgtctgtt
      841 cgccgcctgc agcatattcc gaatactcat cagccggcac atattcgtca gcgattagtg
      901 tatctgctgc atatatatta tcatagtagg cttgtccgtc attcctgccc tggacagtgg
      961 aagcagtcac agccactgta ttctctgacg gcctgtagta caggtcatca ttataatagg
     1021 cacctgtgaa gcggagtgat gagcacgcgc ttgctatcac aagtattacc gggattatgt
     1081 agaaaaacct tttcatttca atctccttct ggttatcaat ccaaaattat acaaattcca
     1141 taccaatttc actctgctgg ccagatattt atcattcgcc aaaataagca aaaattcagc
     1201 atgatgctcc ggcatacttt ccggttcaca agatttgtct atttttacgc aatcttttta
     1261 taagaataac tattataaga aatcattact gcggaatggc caagtttctg acaaacaggg
     1321 atgaaaatta ctcccggtgg tacaatgacc tggttataaa ggctgacatg gctgaaaact
     1381 cggctgtgcg cggttgcatg gtcatcaagc cgtacgggta cgcgatatgg gaaaagatac
     1441 aatcggagct tgaccggatg ttcaaggcta ccggtcatgt caacgcatat ttcccgctct
     1501 ttatccccaa atcattcttc agccgcgagg ctgcccatgt ggagggattt gccaaggagt
     1561 gtgccgttgt aactcattac aggctcaaga actcacctga cggcaagggc attgttgttg
     1621 atcctgacgc ccgtctggag gaggaactca ttgtgagacc cacatcggag acaataatct
     1681 ggaatacata taaggactgg atccagtcat accgtgacct gccgctgctt atcaatcagt
     1741 gggccaatgt agtaaggtgg gagatgcgga cgaggctttt cctccgcacc accgaattcc
     1801 tgtggcagga gggtcatacg gcccatgcca ccagggagga agccgaagag gaggctataa
     1861 agatactgaa tctctatacc gacttcgttg agaattacat gggtgtacct gtagtcaggg
     1921 gggtaaagac tgagagcgag aagtttgccg gtgccattga tacctataca atcgagatgc
     1981 tcatgcagga cggcaaggct ctgcagggcg gaacaagcca cttcctgggg cagaattttg
     2041 caaaggcatt tgatgtgcag tttacaaacc aggagggcaa actcgattat gtctgggcaa
     2101 cctcatgggg cgtaacaacc cgtctgatag gcgctttgat aatggctcac gccgacaata
     2161 acggactggt tctcccgccg aagctggctc ctatccaggt tgtgatagtt ccaatctaca
     2221 aaggcaatga tcagcttgac aagatttcgg atatagccgt tgatatcagg caacagcttg
     2281 aaaaggcagg gtttaccgtc aaatatgaca accgtgatac ccataaaccc gggttcaagt
     2341 ttgctgaata cgagcttaag ggagtgccgg tgcgactggc gattggtccc cgcgatgcgg
     2401 aagatggcac cgtggaggtg gcccgccgtg ataccctgac caaggagacc atcaggcgcg
     2461 aaaacatcgt tactcatgtc accagcctca tggaagagat ccagcagaac atcttcagaa
     2521 aagctgttga attcaggaaa gaaaacacct tcacggctga cacctgggaa gaattcacgg
     2581 atatcatcga aaacaggggt ggtttcgttc ttgcacactg ggacggcacg gcggagagtg
     2641 aagaaaaaat aaaggaactc accaaggcca ccatcagggc aataccattt gacagcaggg
     2701 aagaaaaggg caaatgtatc cttacaggca atccttcaaa ccgcagggta ttgtttgcaa
     2761 gggcctacta aacagatact tgaccaagac aggcatagat cacccgcgaa ttgactccag
     2821 agggtgattt atgtttaatg gattgggttt gtttttgttt gaaataaatc tgtaatttta
     2881 gacaattccg caattgaact gttatggcag atgagattac acaaagcaag gttctgatag
     2941 tagacactct gaaaggaaaa tccgtgctca agcaaaaggt atttgacaat acagcaacag
     3001 ccttcctgat gataaaggat attctgaaga atcttgcccg ggatgtgaac agcactctga
     3061 gtggagttga ccagaggata cgcatggaat atactgacag gagcactttt gatgctcagc
     3121 tcaaggtggc ggccgatctt cttatgttca gcatgcactc aaacattttt cagtttgacc
     3181 gcgagcatcc tgcctggaaa acggcataca tacaaaagaa caggttcaat tcatacagcg
     3241 gcataataaa catatacaac ttcctggcag attcattcaa gtattcgagg cttgatgacc
     3301 ttgggtacct tattgcccgg atctttatca atcatgagaa ccagtgtttt gttgaaggca
     3361 agaggcagat gggtatgctg ttcaccaatt acggcaacga agccttcagt caggaatcgc
     3421 tgaagaagat tgtttatacg gccatcaatt attcacttga atttgacctt ctggttcctc
     3481 cgtatgacac ggtgaaaatt gcttcagtag gccaggcaga atcaaagata cagcattcac
     3541 ggctgataac cggaaaaaga ctcgggttcc agttcaactc tgatgatatt ctggatgaca
     3601 attcaaagcc aaggtgaatc cattcacaaa taggagcatt acaaccctgt tttttttctt
     3661 tctgtcactc ttttttaccc tccccgggcg ccctgttgct gcccaggaca ccactgttcc
     3721 cgacactctt tcaagggctt tctatgactc cctcagggta agggctgaaa aacgcaggct
     3781 gacgagactc ctctatgata tgatcattgt cacgccggct ccgtcagggc atgcgaggga
     3841 agaactggcc agcaccacag cctatgaagc gtacgaggga aggaagatac gcaacataga
     3901 gatcatcagg ctcgacgcct tcggacagaa tatagacaat cctgagggga agactccttc
     3961 acgcgcgcag aggtttatga actccaccta taccaagacc agaaggttcg tgctgagcca
     4021 aaacctcgta ttcaagacgg gagacacaat ttcatcgctg gagctgtctg acaatgaacg
     4081 ccttctcaga cggctgcctt ttattgatga ttcccggata acggtggtgc cggcagacag
     4141 caaccaggct gacattgctg ttattgtgcg tgagaaatac cctgtaggag ccaatctgcg
     4201 cattgatgac atcaggagcg gcagagtggg catttttaca agtaattttg tgggactggg
     4261 ccatgaactg gagatcagta ctccatatga ctttaatgag tatgatgcac cgggaatagg
     4321 gctccgctat tccatcaaca atattgcccg caccttttca aggctgaaca tgcatttttc
     4381 tgacggcctg ggaaccacac acatgggagg tgccttcagc aggaattttg caacatccga
     4441 aaccaggtgg gcatggtcag cctccgtgac attcacccgt accaatgata gaattgacac
     4501 catggccgtg ccggctcctt taaggttcac ctggcaggat tactgggcgg caaggtcatt
     4561 catgctcgac aggagcactg tgacaagact gataatcacg ggcaggtata tgcacaacaa
     4621 cgtattcagg agaccaggca ttgatgcgtt ttcttactac cgcatgcaga actaccagtc
     4681 agttaccggg agcattgcac tctcctcgca gcgattcctc aacacatcat tgatatacag
     4741 ttacggtcgt actgaggata taccatacgg ttatatggcg gaactgctcc tggggcgtga
     4801 aaagaatgag ttcaagtgga ggagctatgc aggaggaacg attgcttacg gcaattttta
     4861 caccggggtt ggttatctct acaccggagc cggcatatca gcattttaca ataacgggca
     4921 cacggagcag ggtctgtttc agggaagcct gcgctatttc acccccctgg ccagggtggg
     4981 gcggtcaagg atgcgtactt tcataaacct gaacttctgc aggggcttta acagatatac
     5041 cgatgaatac ctctatttca gggatgatgg ccttatccgg ggattccgca atgattccct
     5101 gggcggcaac acccgcatcg cagtgtcggt ggaaccggtt ctgttcgtct ataaaccggt
     5161 ggttggattc cggtttgccc tctttgcctt tgccgatgca ggtttattgt ccaggggccg
     5221 gcagatggga tcagactatt ataccgtcgc tgctctcgga ggcggggtca ggatcaggaa
     5281 tgaccagctt gtgcttaata ccctgcagtt gagaatcgcg tggtatccca actcacctcc
     5341 atggtcagag agctccctgc tgactgccaa tagtatcatg cgtctgaggc ctccgggttt
     5401 tgaacctgat cctccggggc tggttccata cctttaggcc cggtgattcc gaagacctcc
     5461 tgcatccggg ctttttcacg gaacaccgca cttttaacat gatgattctt aactttgcag
     5521 aaagcaggtg gttatgctca gcagatttct ggaattttcc gaacgtgaaa agctcttcac
     5581 cggggcaggc aggatactgc ttgccgttag cggcggtatt gattccatgg tgatggcatg
     5641 gcttttcaga gaggccggca ttgagcactc tgtagcacac tgcaactttt cactgagggg
     5701 agatgaatct gacggtgatg aggagttcgt cgcttcatac gcccgcataa acaacattcc
     5761 attcttcagc aaaaggtttg atacactggt atacgccggc acaaacagga tctcggtaca
     5821 gatggcagca agagaactgc ggtacaactg gttcaggtcg ctcctctgct ccgaaggctt
     5881 tgatgccgtg gctgtggcac ataacctgaa tgacaatgtg gaaaccttcc tgatcaacct
     5941 gtcaagggga acaggccttc aaggactgac cggcatgagc ccgcgcacag gagagattat
     6001 caggcccctg ctctttgcaa ccagggatga tattgtatcc tgtgccactg ccaggggcat
     6061 agatttcaga gaagacaggt caaacgccga actgaagtat attcgcaaca ggatcagaca
     6121 caaggttata ccagagctgg gaaaggtcaa tcccgggata ctatcggcag tggttgatac
     6181 tatgaagaac ctcgcctccg cctcggcact gatggatact tatcgttatc agcttggcag
     6241 acaattattc acctcttcgg gtgataccac cgtggttgaa atcagcagtc ttctggaatt
     6301 gaacccgttg gcgccacaca tctttgaact tttccgcccg ttcgggcttt ttccgaaaca
     6361 gactgacgag gtcatctctc ttcttcaggc agggacaggt aaaatggttt acacggcaac
     6421 ccaccgatta ctgaacgaca ggggacggat aatcatttca cccgggaaac cggaagctcc
     6481 atcagaatac cgattcaatt ccattgacga gatgcgtctg tctggtctgt ttagtgcggt
     6541 aaggatcaca gagccttctg aagatgcgct gcctgccacg cgcctgaccg cctccctcga
     6601 tcttgacctg gtgactttcc cggtcacggt gaggcaatgg aagccgggtg acaggttcat
     6661 gccactgggg atgaggcaga tgaagaaaat cagtgatttc ctgatagata tgaaagtgcc
     6721 ggtgacggag aaggaacagg ttatgttgct tctgtcaggc ggtgatgtca tgtgggtaat
     6781 gagttaccgg atagataacc ggttcagggt cacagaggag accgggaaga tacttgttct
     6841 tacactttga accgtcgcac cgggcttcag ccaagagtct ctcagcgttc tccgttgcac
     6901 cgggcttcag ccaagagtct ctcagcgttc tctgtcgcac cgggcttcag gcaagcgtct
     6961 ctcagcgttc tcttatcccg tttattcccc tgtcaatcat catcaggtca gccaggccga
     7021 ttgcaacggc tgattccagg ataaccggga tgcgccgggc aatgcatgta tcatacctgc
     7081 cggttatctc aaggtctgtc atctcaccgg tttcggtatc aaatgttttc tgggtgacac
     7141 ccgtacttgc agcaggtttg actgagaccc tgacgataag gtcattgccg ttggttattc
     7201 caccattgat gccgccggca ttattggttg atgttttgcc cctggcatca ataaacgggt
     7261 cattatggag tgacccatag ctcctcgacg aggcgaatcc tgccccgaac tccaccccgt
     7321 tgactgccgg aatcccgaaa gcaatcattc ccaggagtga ctccactgac atgaacgagg
     7381 gttccccgag ccctttcgga acatttttca ccacacactc caccactcct ccgatggtgt
     7441 cattggtctc cattgccttt gcaatagcc
//
LOCUS       DAOPBK010000130         7426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9002_length_7426_cov_5.153575, whole genome shotgun sequence.
ACCESSION   DAOPBK010000130 DAOPBK010000000
VERSION     DAOPBK010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9002_length_7426_cov_5.153575"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLA17_08130"
     CDS             complement(<1..792)
                     /locus_tag="PLA17_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK85421.1"
                     /translation="MRDNFLEIENVTCGYPGGFMLNSLNLTLQKGAFAGIIGRNGSGK
                     STLFKSITGDIKLTGGSVKLDGISLSGLTLKERARRIATVPQFVETGPITVREHVLMG
                     RVPWLSRFSYSYNKTDNEIAEKYINLTGLNKLSDNPLTGISGGELQLSAIASALTQEP
                     SLLLLDEPTAHLDIHYQAMIMDMLYSLNRAEKLTVLMIIHDLNLAGEYCDYLVMMKDG
                     KICADGTPEEVLSSRNIMDAFGADVLITPHPVTGRPVILRNHGNGP"
     gene            complement(789..1802)
                     /locus_tag="PLA17_08135"
     CDS             complement(789..1802)
                     /locus_tag="PLA17_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPK85422.1"
                     /translation="MSRKRKGMKYTLMLSISALLLVLSSLFSLVIGEVPFSLADVPSI
                     LSDPSSMEHGILTHIRIPRTILAMAIGGSLSLCGVILQGIYRNPLVEPYTLGISGGAA
                     VGVTLAIVTGLAMKGIPLSFFGFAGALVTIFLVYTLSIRNNRMSTGKMLLIGVMISFI
                     SSSVVMLLMSLTNAEQLHGIIFWTMGSLNETDNTVVFTLLGISVIMLGVTILFAVPLN
                     AMRLGEAKARHLGINTTVIIRILFIITSLMTGSCIAVAGIIGFVGLIMPHFSRLIIGT
                     DYRVLLPSAFLNGAIFLIWCDILARIIISPNELPIGVITGIFGGITFIIILSSRRIKA
                     GLL"
     gene            complement(1828..2733)
                     /locus_tag="PLA17_08140"
     CDS             complement(1828..2733)
                     /locus_tag="PLA17_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPK85423.1"
                     /translation="MNINARFLRKTGLLLVTAISVAWLSGCGGSPRTALPGKDTVTFS
                     RVVSLTPSVTRSMYQLEAEDRLAGCSSYCVVEEKDSIVVAGSVVGPDIERIVSLRPDL
                     VILSEFISENDVKTLKKFGLRVEVFRSPESFDELCLQFIRLGQLTGKEKEAEQIAADI
                     KNEVRLLSDEWEQKSPKPGVFMQIGSSPIFTVLPGTYMDDYIKFCGAENIAADLTNGI
                     VGREFVVAKNPDYIFIVTMGVAGDLEKEQWSRFSLMNAVRQNNIHILDSRIACEPTPE
                     NFIKTLRIMAGYMGKELDGQPLSDQ"
     gene            complement(2776..3840)
                     /locus_tag="PLA17_08145"
     CDS             complement(2776..3840)
                     /locus_tag="PLA17_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85424.1"
                     /translation="MKTNLLQKAMLLCAGAIIMLVSCQKDPDDDITAKNLKGMFVVCE
                     GNFGSAEGDITWYNPDDGKTVKSLYYSANSTELGDIAQSFVIADTLGFIAVNNSQKVT
                     VINMKSFVAVKTLNNFSYPRSITRANEKTVYVANGNGFADNYIYSIDLATLKKTDSLV
                     VTTGPESLITAGNKVYATISGGWNNDGNTVIEIDPETFEITKTYTVGSCPVDLVADNN
                     MNIWVYCKGIPDYSAYPDISYTGMGLCKIDRTTGNVTTFPFTEMVSPGIYNVAASPDG
                     KTIYYLNNGLFAVESAATSLPASALINGSFYGIDVDPKSGNLVGLDAVNSKAVIFNTN
                     GIEQDSFETGDFPNSVVFSY"
     gene            complement(3861..5813)
                     /locus_tag="PLA17_08150"
     CDS             complement(3861..5813)
                     /locus_tag="PLA17_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK85425.1"
                     /translation="MTNVTVLSIVTVQKLTKQLMRTATRLTLAAVLSFPGVTGAQSIT
                     DTLIHIPDVVVNASRTDHFRHDVKTEEFRDSILNQYAGESLVRLLSGQTALNIKAYGV
                     GGGASSISIRGASASQVQVNWNGFPINSVTLGSSDISMIPAAGFDRISLVYGASGALY
                     GSGTFGGAVSLNSELTPERGISGSAYIGAQSLRTLNGGFTFKAGSDRLAWKVFAWGTV
                     SENEFTYYDYIRQTEHRQTDGDWHDYGMIHNLAFRLSPASLLEAGLWYQEKEYDIPSR
                     IGSTTYEQQSDSTLKLYLGYRYIGNRWSLKIRAARFDDLQTYWQKPAPDSPVNSIDSR
                     IAALQHHGDINFRYYMPSGLSFDAGLTGSMVTADVSAYGDKKLEKGIALFTGLKYTFD
                     RLTLQSALRKEWFNTFSSGLLPSFGLKWDAIPGQWVLRANYSKKFRKPTFNDLFWIPG
                     GNPDLNPENGYTIEAGSENSFHVSEKSVIETDITPYYTHVNDMIVWRPGGAYWSPKNY
                     QEVRSVGIDLSAGFTMKHEKIKFSSMLKVGLNHSVANEEDGQEKLTMLYSPLFVSSWK
                     NSLTAGIFDLEVTHGFTSSRHYSDDRLLDPYNLIDIRAGVNIKAGKGKIGMHLSCNNV
                     TNTTYELIKLYPMPGRYWSAKINYEF"
     regulatory      complement(5911..6137)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6457..>7426
                     /locus_tag="PLA17_08155"
     CDS             6457..>7426
                     /locus_tag="PLA17_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPK85426.1"
                     /translation="MPGAAVSLKSILKGAISDSYGYYTITGVPAGTYELIATYIGYSP
                     EAKQISVEPGRTTVVDFSLEAGIELSEITVTGQLQGQSKALNTQMNKGNITNIISSDQ
                     VGRFPDANIGDALKRIPGINVQYDQGEARFGNIRGTAPQYNSVMVNGERIPSAEAEDR
                     TVQLDLIPADMIQAVEVNKAVTPDMDADAIGGSVNLVSRSAPYDRRVSMTLGSGYNLL
                     ASKPIYNGAVILGDRFFNNKLGVIIGASYHNHQLGSDNIESEWDYTDDNNKDLSAFAE
                     EIQIRQYYLQRIRQSYSLSLDYKINGNHTLFANGIYNHRNDWENRYR"
BASE COUNT         1933 a         1758 c         1719 g         2016 t
ORIGIN      
        1 cggcccgttc ccgtggttcc tgaggataac aggcctgccc gtgaccggat gaggtgtgat
       61 cagcacatca gctccaaatg catccatgat attccgggaa gagagtactt cttccggcgt
      121 gccgtcagca catattttac catctttcat catcacaaga tagtcacaat actctcctgc
      181 aagattcaga tcgtgaataa tcatcaagac cgttagtttt tccgccctgt tcagactgta
      241 aagcatatcc atgatcattg cctggtaatg aatatcgagg tgcgcggtgg gttcatcaag
      301 cagcaggagg gaaggctcct gcgtcagggc tgatgcaatg gctgaaagct gaagctcacc
      361 gccgctgatg cctgttaaag ggttatcact gagtttgttc aggcccgtga gattgatata
      421 cttttcagct atctcattat ccgttttatt atatgaatag ctgaacctgc tgagccaggg
      481 aacccttccc ataagaacat gttccctgac agtgatcggc cctgtctcaa caaattgcgg
      541 tactgtggca attctcctgg ccctctcctt cagtgtcagg cccgacaggg agatgccgtc
      601 aagtttcact gatccgccgg taagctttat atcaccggtt atgcttttga agagagtact
      661 cttgcctgag ccgttgcggc caatgatgcc tgcaaaagcc cctttctgca gagtcaggtt
      721 cagtgagttt aacatgaaac ctccggggta gccgcaggtg acgttctcta tctccaggaa
      781 attgtccctc atagtaatcc cgcttttatc ctccggctgc tgaggatgat tataaaggta
      841 atgcccccga aaatgccggt tataacacct atcgggagtt cgttgggaga gataatgatc
      901 cttgccagga tgtcacacca gataaggaat atggctccgt taaggaatgc agagggaagc
      961 agtacacggt agtcagtacc aataatgagt cttgaaaaat gcggcatgat aagccctaca
     1021 aatccaatta taccggctac tgcaatgcaa ctgccggtca tcagggaggt gataatgaaa
     1081 agaatcctta tgatgacggt tgtattgatt cccaggtgcc ttgccttagc ctctcccaac
     1141 cgcattgcat tcaacggaac agcaaagaga atagtgaccc cgagcataat aacggaaatt
     1201 cccaaaaggg tgaaaacaac agtgttatct gtttcattca gtgaccccat ggtccagaat
     1261 attataccgt ggagctgttc agcgttggtt agagacatga gcagcatcac tactgaagag
     1321 gaaatgaagc tgatcattac ccctatgagg agcatttttc cggtactcat tctgttgttc
     1381 ctgatactca gggtgtaaac aagaaaaatg gttaccagcg caccggcaaa gccgaaaaat
     1441 gagagcggga tacctttcat ggcaagaccg gtcacaatgg caagtgttac gccaacagca
     1501 gctcccccgg aaattccgag cgtgtaaggc tctaccagcg ggttgcggta aataccctgc
     1561 agaatgactc cgcaaaggct gaggctgccc ccgatggcca tggcaagtat tgtccgcggg
     1621 atgcgtatat gcgtcagtat cccgtgttcc attgatgaag gatcagacag tattgacggt
     1681 acatctgcca gagagaaagg tacttcacca atgaccaggg agaagaggga tgacaatacc
     1741 agcaacagtg ctgatattga cagcataaga gtatatttca tcccttttct tttcctgctc
     1801 atctgtctga cctgcctgtg agccccgtca ttggtcagac aagggttgtc cgtccagttc
     1861 ttttcccatg taacctgcca ttattctcag ggttttgata aaattctctg gcgtgggttc
     1921 acaggcaatt ctggaatcta atatatgaat attgttttgt ctgactgcat tcatcagtga
     1981 aaaacgcgac cattgctcct tttccaggtc tccggcaacg cccatggtta caataaaaat
     2041 gtagtccgga ttctttgcca caacaaactc gcggccgaca ataccgttcg taaggtctgc
     2101 agctatattc tccgctccgc aaaacttaat ataatcatcc atgtaggttc cgggcagtac
     2161 cgtgaagatg ggagagctcc caatctgcat gaatacacca ggtttggggc ttttctgttc
     2221 ccactcgtca gaaagaaggc gaacttcgtt tttaatatcc gcagctatct gttcagcctc
     2281 tttctccttg cctgtcaact gccccagcct gataaactgt aaacagagtt catcaaaaga
     2341 ttccggtgaa cggaatactt caactctcag cccgaacttt ttaagtgttt ttacatcatt
     2401 ttcggagatg aattctgaca gtattaccaa atcgggtctc aggcttacaa ttctttctat
     2461 atcaggccct actaccgatc cggctacaac gatactgtct ttctcttcaa caacgcagta
     2521 gctggagcag cctgcaagcc tgtcttccgc ctcaagctgg tacatgctcc tggtaactga
     2581 gggtgtcagt gaaacaaccc ttgagaaagt aacagtgtct ttgccgggga gagccgttcg
     2641 cggggaaccc ccgcatcctg aaagccaggc aacagaaatt gccgtgacaa gtaacagtcc
     2701 tgttttacgc agaaaccttg cgtttatatt catgccggta tttataaaac cttacacagg
     2761 aagtttgtca gtaattcaat aactgaagac aactgaattg gggaagtcac ctgtttcaaa
     2821 gctgtcttgc tcaatgccgt tggtattgaa gataacagcc tttgagttaa cagcgtcaag
     2881 cccgaccagg tttccactct tcggatcaac gtcaataccg tagaatgagc cgttgataag
     2941 agctgaagcg ggcagggatg tagcagctga ctccactgca aataacccgt tgttcagata
     3001 atagattgtt ttgccgtcag gtgatgctgc aacattatat atcccgggtg agaccatttc
     3061 agtaaacggg aatgttgtga cattgcctgt ggttctgtct atcttgcaca accccatgcc
     3121 ggtatagcta atatccggat aggctgagta atcaggaata cctttgcaat agacccagat
     3181 gttcatgtta ttgtcggcaa caagatcaac aggacatgag cccacggtat atgtctttgt
     3241 gatttcgaag gtttccgggt ctatttcaat aacggtattg ccgtcgttgt tccagccgcc
     3301 ggagattgtt gcatacactt tgttgcctgc tgttattagt gactcaggcc ccgtggtgac
     3361 cacgagcgag tcagtttttt tgagggttgc aaggtcaatg ctgtagatgt aattgtcagc
     3421 aaacccgttt ccgttggcca cataaacggt tttttcattt gcacgcgtaa tactgcgggg
     3481 ataagagaaa ttattcaggg ttttaaccgc gacaaagctc ttcatattta tcacggttac
     3541 cttctgagag ttgtttactg caataaagcc cagcgtgtct gcaatgacga atgattgggc
     3601 tatatcaccg agctccgtgc tgtttgcaga gtaataaaga ctcttaaccg ttttgccatc
     3661 atcaggattg taccaggtga tatcaccttc agcgcttcca aaattccctt cacaaacaac
     3721 aaacatcccc ttcaggtttt ttgcggtgat gtcatcatca gggtcttttt ggcatgagac
     3781 aagcatgata attgctccgg cgcacaggag catagctttt tggagaagat tggttttcat
     3841 aataatcatt ttaaaagtgg ttagaattca taatttattt tggctgacca ataccttccg
     3901 ggcattgggt agagctttat cagttcatag gttgtattgg ttacattgtt acaggataag
     3961 tgcataccga tctttccctt tcctgccttg atattgacac cggctctgat atcaatcaga
     4021 ttatagggat caagaaggcg gtcatcgctg taatggcgtg atgatgtaaa accatgtgta
     4081 acttcaagat caaatatccc ggctgtcaga ctgttcttcc atgatgagac aaacagggga
     4141 gagtatagca tggtgagctt ttcctgtcca tcttcctcat ttgcaactga atggttcaga
     4201 ccgaccttta acatggagga aaacttgatt ttttcatgtt tcatggtaaa acctgcagac
     4261 aagtcaatgc ccacagacct gacctcctgg tagtttttgg gcgaccagta agctcccccc
     4321 ggccgccaca ctatcatgtc attgacatga gtataataag gagtgatatc tgtttctatc
     4381 actgactttt cactgacgtg gaaactgttt tctgaacctg cttcaatggt gtagccattt
     4441 tccgggttga ggtccggatt gcctcccggt atccagaaga ggtcattaaa cgttggtttt
     4501 cggaatttct tcgagtaatt tgcacgcaga acccattgcc ccggaatggc atcccatttc
     4561 aatccaaacg aaggcaggag tcctgagctg aaggtgttga accactcttt gcgtaatgca
     4621 ctctgcaggg ttaaacggtc aaaggtgtat ttcagtccgg tgaagagggc tatccctttt
     4681 tcaagcttct tatcaccgta ggctgataca tctgcagtga ccattgagcc tgtaagcccg
     4741 gcatcaaatg agaggccgga aggcatgtaa tacctgaagt taatatctcc gtgatgctgt
     4801 agcgcggcga ttcttgagtc aatgctgttt accggtgagt caggagcagg cttttgccag
     4861 tatgtctgaa ggtcatcgaa ccgtgctgcc ctgatcttaa ggctccagcg gttgcctata
     4921 tacctgtacc ccaggtagag tttcagtgtg gagtctgact gctgttcata ggttgtggag
     4981 cctatccgag acggaatatc gtattccttt tcctggtacc ataacccggc ttcaagcaga
     5041 gaagccggcg acagtctgaa tgccaggttg tgaatcatgc cgtagtcatg ccagtcgcca
     5101 tcggtttgcc ggtgttcagt ctgccttata taatcataat aggtgaattc attttctgag
     5161 actgtgcccc aggcaaacac tttccaggcg agcctgtcac tgcctgcctt gaatgtaaac
     5221 cctccgttga gtgtgcgcaa tgactgtgca cctatgtaag cgctgcctga aatgcccctt
     5281 tccggtgtta actcactgtt aagactcacg gctccgccga aagtgccgct tccatagaga
     5341 gctcccgagg ctccgtacac cagagatata cggtcaaatc cggctgcagg gatcattgat
     5401 atgtctgagg agccgagcgt cacggaattg atcgggaacc cgttccagtt aacctgcacc
     5461 tggctggctg aagccccgcg aatggagatg gatgatgccc cgcctccaac gccgtatgcc
     5521 ttaatattca gtgcagtctg accgctcagg agccttacga gtgactcccc ggcgtactga
     5581 tttaatatac tgtccctgaa ttcttcggtt ttaacatcat gcctgaaatg atcagtacgt
     5641 gaggcgttta caacaacatc gggaatatgt atcagggtat ctgttattga ctgggcgcct
     5701 gtgactcccg gaaatgataa aactgcagcc agagtcagcc tggttgcggt tcgcatcaat
     5761 tgctttgtca gcttctgcac ggttacaata cttagaacag tcacatttgt catggtcttt
     5821 aaaatccatg acctcaattc aaattctcgt aaccttttga gagacagggg tagaaaaacc
     5881 agtttctgct ctttgccttt cacccgaaag ctacgaggtc aatttacagg caggtcttct
     5941 ggctcatcct gttccggctg ccttcccatt tcacattttg tgtaacagtg gcggggggtg
     6001 ccggaacaat tatcggattt acagctgcgg gtacagctcc cggtttcctg caaccgttcg
     6061 gtttacaggt cacgggattc cctattcact caggccggag cggttcaggc ctgagcacct
     6121 gaaatcgtca ggcaaatata tacattttat tattgcaaca tgaattctgt gccaatgctg
     6181 actctattta ctatcagtcg cagatccgca cttgaaaatc cgtctggcat gctcaggcag
     6241 aaataatgag ccggatcatt aaaatgtatc aatcctttaa tatttgcgta aactttttgt
     6301 aataaactga aagtaatttt gccatgaatt ccaaaataaa tcatgacaca tttaaaatct
     6361 tacctatctg tgcttctgac agcattgaca atatgttctc aggcacaaca atcagggatt
     6421 attaaaggaa gagtcctgga taccgacaac ctttcaatgc ctggtgcagc tgtatcctta
     6481 aaatcaattt tgaaaggcgc catcagtgac agctacggtt attacactat taccggagtt
     6541 cccgccggga catacgaact gattgccaca tacatcggct atagccccga agcaaaacag
     6601 atttcagtcg agcctggcag gacaactgtt gtcgacttca gcctggaagc aggtattgaa
     6661 ttatccgaaa taactgtgac cgggcagctg caggggcagt caaaagcttt aaatacacaa
     6721 atgaataagg gtaacatcac caacattatt tcatctgacc aggtaggccg attccccgac
     6781 gccaatatcg gtgatgccct taagagaatt ccgggtatca atgttcagta tgatcagggc
     6841 gaagcgcgct tcgggaacat aaggggaaca gcaccgcaat acaactcggt gatggtaaac
     6901 ggcgaacgca tcccttctgc cgaggctgaa gaccggacgg tgcagctcga tctgattcct
     6961 gccgacatga ttcaggctgt cgaggtgaac aaagcggtca cacccgatat ggacgctgat
     7021 gccattggtg gttcggttaa cctggtatcg cgctctgctc catacgaccg tcgtgttagt
     7081 atgacacttg gatccgggta taatctcctt gcctcaaagc caatatataa tggcgctgtt
     7141 atacttggcg accggttttt caacaataag ctgggcgtaa ttattggtgc ctcttaccac
     7201 aatcaccagc tgggaagtga caatattgaa tcagaatggg attatactga tgacaataat
     7261 aaagatcttt cagccttcgc agaagagatt caaatacggc agtactattt acagcgtatc
     7321 cgtcaaagtt attcactgag tcttgattat aaaattaacg ggaaccatac attgtttgcc
     7381 aatggtatat acaaccatcg taacgactgg gaaaaccgtt acagga
//
LOCUS       DAOPBK010000131         7398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9048_length_7398_cov_5.350129, whole genome shotgun sequence.
ACCESSION   DAOPBK010000131 DAOPBK010000000
VERSION     DAOPBK010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9048_length_7398_cov_5.350129"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..585
                     /locus_tag="PLA17_08160"
     CDS             16..585
                     /locus_tag="PLA17_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPK85427.1"
                     /translation="MHSATKYLSGHGNITAGVICGNNDKLMQSAVEYRKYVGHMLSPD
                     DAYRLQTQMASFELRFRQQCDNAAAVASMLQSSDRVAGVLYPGLESHETHAAAKELTE
                     GKGYGAMITFDLAGASPEAKRERRDRFIAAVYPEIRLIPTLGDNHTILMPVEAVWGTK
                     YPEPGMIRLSVGYEETDRLAAIIGRGLKG"
     gene            complement(586..1248)
                     /gene="ung"
                     /locus_tag="PLA17_08165"
     CDS             complement(586..1248)
                     /gene="ung"
                     /locus_tag="PLA17_08165"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPK85428.1"
                     /translation="MEVRIEAGWKEKLSGEFEKEYFRTLTEFVREEYRTRQVFPPGNK
                     IFNAFDLCPFDSVRVVIIGQDPYHNYGQAHGLCFSVTDGTEFPPSLVNIFKELNRDLG
                     VPVPESGNLERWAAQGVLLLNAILTVRAHQALSHQNRGWEKFTDAAISALNRDRENLV
                     FLLWGAYAQNKGASIDESKHLVLRTVHPSPLSASRGFFGCSHFSKCNQWLVARGMEPI
                     EW"
     assembly_gap    1432..1531
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1768..2136)
                     /locus_tag="PLA17_08170"
     CDS             complement(1768..2136)
                     /locus_tag="PLA17_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPK85429.1"
                     /translation="MKGLWSILLLIVSNSFMTIAWYGHLRYQNVSKNALLGLLGIVLA
                     SWGIALFEYFFQVPANRIGYRGTGGPFSLVELKLIQEVISISVFVLFTLLFFRTETFR
                     LNHLFAFICILGAIYFTFKG"
     gene            complement(2133..2612)
                     /locus_tag="PLA17_08175"
     CDS             complement(2133..2612)
                     /locus_tag="PLA17_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK85430.1"
                     /translation="MPEISFNITPADRAVFEKILASSGFFYDFEIEVALSLADETFTH
                     GEEKSGYYWIKLVEEGEIIGFATFGPNPSSVHSWDIYWIAMHEEQRNKHYGSLILKES
                     ENRILSLGGKIAWAETSGRPLYEPTRHFYERNGYELEATLREFYGPGDSKLVYRKEL"
     gene            complement(2623..3588)
                     /locus_tag="PLA17_08180"
     CDS             complement(2623..3588)
                     /locus_tag="PLA17_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPK85431.1"
                     /translation="MTKRCLIIYNEPAPDALPDELDVLDQVNFINETLQSLGYQVECR
                     GITENFFSEIREMAGGEFEFVFNLVESVGNKAEILYFIPGLLNMYHIPYTGCPVEATF
                     ITASKVLARKIMKASGIPVAGGYRVSEASQLVPGKKYILKPVWEDGSLGITEESVFTF
                     NGTPPEILRGRNDQHWFIEEFIEGREFNVSVISGPDGPEMLPPAEMLFENYPDDIPRI
                     VSYKAKWVEETFQYENSRRHFPEELSEKLMNNLRAAVNGCWHTFGLKGYARVDMRVDA
                     NENVYVLEVNANPCISPDSGFISAAVHAGYTHQEIIRHIINDLNA"
     gene            complement(3588..4562)
                     /locus_tag="PLA17_08185"
     CDS             complement(3588..4562)
                     /locus_tag="PLA17_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85432.1"
                     /translation="MRIGLTYDLRSWYLERGYTMEETAEFDKEETVAAIENELVRLGY
                     DTVRIGNIFQLVERLAAGERWDMVFNIAEGLFGDGRESVVPALLDQYRIPYVFSGPVV
                     MGVSLNKYLTRLVVAAAGVPVCPGIIANSLHDLDDLSSLEFPLFVKPVAEGTGKGIST
                     KSVIRDRESLVPLVSGLLKAFNQPVLIEEYLPGREFTVGVTGNGAEAKVTGGMEVICR
                     DNLPYSMEVKENYENYVRYRVYDEEVRAECNAVALGAWRALGAVDAGRVDMKADRHGR
                     ICMIEVNPLAGMNPVHSDLPLLSRMNGLAFSTLMEMIINSAKKRYGLA"
     gene            complement(4570..5814)
                     /locus_tag="PLA17_08190"
     CDS             complement(4570..5814)
                     /locus_tag="PLA17_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPK85433.1"
                     /translation="MNANRKEATAARVESRLMGCSSDEEPGAGTMIFPQIGSHDCLVS
                     NRSSEFIRRYFPLANAESWNDWRWQLRNSFTTAEALSRVMTLTGDEINTLNRLKGRLP
                     LRITPYYLSLIYDSGPWSPLRRTMIPTSDELIITDAEKADPLNEMGSSPVPGIVHRYP
                     DRALFTVTQFCSSYCRYCTRSHSVGKLGHLTKVEWEQALDYLREHTEIRDVIISGGDP
                     LTMNDSKIEYLLSNLRAIKHIEILRIGTKVPAVLPQRITPQLVNMLKKYHPLFLSIHF
                     THPDEMTPETRRACEMLADAGMPLGSQTVLLKGINDDPATMKSLMHKLLTVRVRPYYI
                     YQCDLIPGSSHFRTTIEKGIDIIENLRGHTSGYAVPQFVVDAPGGGGKIPVLPDYVVE
                     SGPEKWVLRNYKKNRYTYPNKK"
     gene            complement(6069..7268)
                     /locus_tag="PLA17_08195"
     CDS             complement(6069..7268)
                     /locus_tag="PLA17_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPK85434.1"
                     /translation="MTEQEFDDRVKRLFDDHEKLISRPNIRKEGGNGIFDRYLHPIIT
                     RDHTPVFWRYDLDYRSNPHLMTRLGINTTFNAGAIKLDGKYCLAIRTEGFDVKSFFAI
                     AESPDGINNWRFRNRPIVMPVTGDPEMNVYDIRLTRHEDGWIYGLFCAERKDKSKPDD
                     SSAALASCGIARTRDLDTWERLDDLASGAAQQRNCVLHPEFVNGKYMIYTRPQSGFIE
                     TGDSGISAGFCDSMEHAVLGEEILVDPRQYHTVKEVKNGQGPAPLKTEYGWIHLAHGV
                     RRTAAGLRYTLYLFVTSLEEPWRVIKRPSGHFLEPLEDERVGDVSNVVFSNGWIADDD
                     GTLFVYYASSDTRMHVATTTMDKLIDYVMNTPEDAGRTQLCAAQRDRMIENNLKIMAQ
                     KYPNIRF"
BASE COUNT         1745 a         1831 c         1825 g         1897 t
ORIGIN      
        1 ctgtaatgtt gccatatcca cagcgccacc aagtatctct ctggtcatgg caacattaca
       61 gccggtgtga tatgtggcaa caatgacaaa ctgatgcaga gcgcagtaga gtaccgcaaa
      121 tatgtggggc atatgctttc gcctgacgat gcctatcgac ttcaaacaca gatggcatct
      181 tttgagcttc gtttcaggca gcaatgtgac aatgcggcag cagtggcgtc aatgctgcag
      241 tcatctgacc gggttgccgg cgtgctttac cccggacttg aaagtcatga aacccatgca
      301 gcagccaaag agctgacgga agggaaggga tatggtgcta tgatcacctt tgatcttgca
      361 ggagcttcgc cggaggcgaa gagggagagg cgcgacaggt ttattgcagc tgtttatccg
      421 gaaatcaggc ttattcccac gctgggcgac aaccatacga tactgatgcc tgtggaggca
      481 gtatggggaa caaagtatcc tgaacccgga atgataagat tatctgtagg ttacgaagaa
      541 actgacaggc ttgccgcgat tatcgggcgc ggactgaagg gctgatcacc attctattgg
      601 ttccatccct ctggcaacaa gccactggtt gcatttgctg aagtgtgagc agccgaaaaa
      661 gccgcgtgaa gctgagaggg gagagggatg tacggtcctg agcaccaggt gctttgactc
      721 gtcaattgat gcccccttgt tttgtgcata ggcgccccac agcaggaata ccaggttttc
      781 tctatcacgg ttcagtgcac ttatggcggc atcggtaaac ttttcccatc ccctgttctg
      841 gtgcgagagc gcctggtgtg ccctcacggt gagaatggca ttgaggagaa gcacaccctg
      901 ggctgcccag cgttcgaggt tgcccgactc tggcaccggc accccgagat cgcggttcag
      961 ctccttgaag atattcacca ggctgggagg gaactcagtg ccgtcagtga ccgagaaaca
     1021 gagtccgtga gcctgcccat agttatgata gggatcctgg cctatgatca caaccctgac
     1081 actgtcaaaa gggcagagat caaatgcgtt gaagattttg ttcccaggcg ggaatacctg
     1141 cctggtacgg tactcttccc ggacaaattc cgtcagagtc ctgaagtact ccttttcaaa
     1201 ctccccgctg agtttttctt tccagccggc ttctattctt acctccataa ttgcatgcac
     1261 agtctatata gtgttaaata tattgaattt tcattgaagt gtcattccgt tatgaaagaa
     1321 gggataaaaa cgagttatat tataatctca aattcaaaaa aaatgattga tctgcgcagt
     1381 gataccgtaa cccggccctc agccggaata cttgagagga tgatgaaagc cnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncttgagagg atgatgaaag cccgggttgg
     1561 tgacatgatt ttcggggaag acccctggat caatgagctt gagggggata tcaattaaga
     1621 tacgcgagta aacaagcaac gttcacaccc ctgggtcaat gagcttgagg gggatacctg
     1681 ccctgaaaac tggtgacaga agcataagga tggtcactca ccttgatgtg agcgaaggag
     1741 agataaaaga agcctgtgat atagttgcta acctttgaag gtaaagtaga tcgccccgag
     1801 aatgcagatg aaggcaaaaa ggtggttgag cctgaatgtc tcggtgcgga agaagagcag
     1861 ggtaaaaaga acgaatactg atattgatat cacttcctgg atgagtttga gctcaaccag
     1921 tgagaaagga ccaccggtcc cccggtagcc tatcctgttt gccggtacct ggaagaaata
     1981 ttcaaacagt gctatccccc agctggccaa cacgatgccc aaaagcccaa gcagggcatt
     2041 tttagaaacg ttctggtacc tgagatgccc gtaccaggct attgtcatga atgaattgga
     2101 gactatcagc agcagtattg accagagacc tttcataatt ccttccggta aacaagtttg
     2161 gagtcaccgg gaccgtaaaa ctcgcggagg gtagcctcaa gctcatagcc gttcctttcg
     2221 tagaaatgcc tggtgggctc gtaaagaggt ctgccggagg tctctgccca cgctatcttt
     2281 cctccaaggg atagtatccg attctcactc tctttcagga tcagggagcc ataatgcttg
     2341 ttcctctgct cctcatgcat ggcaatccag tatatgtccc atgagtggac agatgaagga
     2401 tttggcccga aagtggcaaa tcctataatc tctccctctt ccacgagctt aatccagtag
     2461 tatccgcttt tttcttctcc atgggtaaaa gtctcgtcgg caagggaaag agctacctct
     2521 atctcgaaat cgtaaaaaaa acctgatgaa gcaagtatct tttcaaatac agctctgtcg
     2581 gccggggtta tattgaatga tatttcaggc atggataatt gttcaggcgt taaggtcgtt
     2641 tatgatgtgc ctgataattt cctggtgggt atatccggca tgtactgcag ctgaaatgaa
     2701 tccgctgtca ggtgagatac acggatttgc attcacttca agcacataga cattttcatt
     2761 ggcgtcaacc ctcatgtcaa cgcgcgcata gcccttcagg ccgaaggtat gccagcaacc
     2821 gttgacggca gctctcaggt tgttcatgag cttttcgctc agctcttcgg ggaaatgccg
     2881 cctgctgttt tcatactgga aggtctcttc cacccacttc gccttataac ttactatccg
     2941 gggaatatca tccggatagt tctcgaagag catttccgcc ggcggcagca tttccggccc
     3001 gtcaggaccg gatatgacac ttacgttgaa ttcccgtccc tcaataaact cctcgatgaa
     3061 ccagtgttga tcattcctgc ccctgagaat ctcaggaggg gtgccgttaa aggtaaacac
     3121 tgactcttcc gtaataccca gtgaaccgtc ttcccatacc ggcttaagga tatatttctt
     3181 tccgggaaca agctgcgagg cttctgacac cctgtaacct ccggcgacag gtattccgct
     3241 ggccttcatg atctttcgtg ccagcacctt gctggcagta atgaaggtag cttccaccgg
     3301 acagcctgta tagggaatgt ggtacatgtt cagaagtccg gggataaaat agagtatctc
     3361 cgccttattg ccgaccgact ccacgaggtt gaatacgaat tcaaattccc cgccggccat
     3421 ctccctgatc tcactgaaaa aattctcggt tattccgcgg cattcaacct gatatccaag
     3481 actctgcagt gtctcattaa tgaaattgac ctgatcaaga acatcgagct cgtccggcag
     3541 ggcatcagga gccggttcat tgtatattat caggcacctc tttgtcatta tgcaagtccg
     3601 taacgtttct tcgcagaatt gattatcatc tccatcaggg tactgaaggc aagtccgttc
     3661 atgcgcgaga gcaggggcag gtctgagtga acagggttca ttcctgccag cgggttaact
     3721 tcgatcatac agatccttcc gtggcggtct gccttcatat caacacgccc ggcgtctaca
     3781 gctccaaggg cacgccaggc gccaagggca actgcgttgc actctgccct cacctcctca
     3841 tcatataccc tgtagcggac ataattctca tagttttcct tgacctccat tgagtaggga
     3901 aggttatccc tgcagatcac ctccatgccg ccggtaactt tcgcttcggc accattgccg
     3961 gtaacaccca cggtaaactc ccttccgggg agatactcct caataagcac aggctggtta
     4021 aaggctttca gcagcccgct gacaagcgga accagggatt cacggtccct tatcacgctt
     4081 ttggtggaga tgcccttgcc cgtaccctcg gcaacgggtt ttacgaagag cgggaactca
     4141 agtgaagaga ggtcgtcaag atcatggagg ctgtttgcta ttatcccggg acaaacaggc
     4201 acaccggcag cagcaaccac cagcctggtg agatatttat tgagtgatac gcccatcacc
     4261 accggcccgc tgaagacata gggaatcctg tattggtcaa gcagggcagg cacaaccgat
     4321 tcacggccgt cgccaaagag cccctcggca atgttgaaca ccatgtccca ccgttcaccg
     4381 gctgccagcc tctcaaccag ctgaaagata tttccaatgc gtacggtatc gtagccaagg
     4441 cgtacaagct cattctcaat ggcggccacg gtctcctcct tgtcaaattc agccgtctcc
     4501 tccatggtat aaccccgttc aaggtaccac gaacggaggt cataggtgag tccaatcctc
     4561 atcccttaac tactttttgt tggggtaagt gtagcggttc tttttatagt ttctgagaac
     4621 ccatttttcc gggccgctct cgactacata atctggcagg acaggtatct tgccgcctcc
     4681 gccgggagca tcaaccacaa actgtggtac tgcgtatccg ctggtatgtc cgcggaggtt
     4741 ttctatgatg tcaatgccct tctcgatggt ggttctgaag tggcttgagc ccggtatgag
     4801 gtcacactgg tatatgtagt aaggccttac gcgcacggtg aggagcttat gcatcaggct
     4861 cttcatggtt gccgggtcat cattgatacc tttcagtagt acagtctggc ttcccagggg
     4921 catgcctgca tcggcaagca tctcacatgc cctgcgcgtt tcgggggtca tctcatcagg
     4981 atgggtgaag tggatgctga ggaagagcgg gtggtatttt ttcagcatgt tcaccagctg
     5041 cggagttatt ctctgtggca aaacagccgg tactttcgtt ccgatcctca gtatctcgat
     5101 atgtttgatt gccctcaggt ttgaaagcag gtactcgatc ttgctgtcat tcatggtgag
     5161 agggtcacct ccgctgatga tgacatcacg aatctcagtg tgctccctga gataatcaag
     5221 ggcctgttcc cattcaactt tggtcaaatg gcccagtttg cccactgaat gtgagcgtgt
     5281 gcagtacctg cagtatgatg agcagaactg agtcaccgta aagagggccc tgtcagggta
     5341 acggtggact ataccgggca cagggctgct tcccatctca ttgagagggt cagccttttc
     5401 ggcatcagta atgatcaact catcactcgt cggtatcatg gttcggcgaa gcggtgacca
     5461 cggcccggag tcatagatga gtgacaggta atatggagtg atcctgagcg gcagccttcc
     5521 tttcagcctg ttgagggtat tgatctcgtc accggtcagt gtcatgacac gggagagggc
     5581 ctcggcagta gtgaaagagt tgcgcagctg ccagcgccag tcattccagg attcagcatt
     5641 tgcaaggggg aagtagcggc gtatgaattc cgaactccgg ttgctaacta aacaatcgtg
     5701 ggatccgatc tgcgggaaga tcatggtacc cgcgccaggt tcttcgtctg aagaacaacc
     5761 catgaggcgg ctctcaacac gagcagccgt tgcctctttt ctgttagcgt tcatacttcg
     5821 gtaaaatact ttaccttttt ttctggttgt taattaatcg ttttagttat taaaaagttt
     5881 cattaaatct ccgcaaagtt attaattgga taaccttcgg ttttctgctc cggtcgaaag
     5941 atattaacat gccaatcatg cctgctaata tctggttttc aattttatag cctgtctgaa
     6001 tccgggttat tcacagcatt cattcaggtg gtgaaatcga tgcccggaat ttgaggcgca
     6061 aacatatatc aaaatcttat atttggatac ttctgtgcca ttattttcag attattttct
     6121 atcatccggt cgcgctgcgc tgcacataac tgtgtgcggc ctgcatcttc cggtgtattc
     6181 atcacataat caattagttt atccattgtt gttgttgcca cgtgcattct cgtatctgag
     6241 ctggcataat aaacaaagag cgtgccatca tcatcggcaa tccatccgtt gctgaagaca
     6301 acatttgaga catcgcctac tctttcatcc tcaagtggtt caaggaagtg ccccgagggt
     6361 cgctttataa ctctccacgg ctcttccagg gaggtcacaa agaggtagag ggtgtatcgg
     6421 agtccggcag ctgttctcct gacgccgtgt gcaaggtgaa tccagccgta ttctgtcttc
     6481 aggggtgccg gtccctggcc gttcttcacc tcttttaccg tatggtactg ccgcggatcg
     6541 acaagtatct cctcaccgag cactgcatgc tccatgctgt cacagaatcc tgctgatatg
     6601 ccgctgtcac ctgtctcgat gaaacctgac tgtggcctgg tgtagatcat gtatttgccg
     6661 ttgacaaact cggggtgcag aacacaattt ctctgctgtg ccgctccgga agcgaggtcg
     6721 tcgagtctct cccaggtgtc gaggtccctg gtacgtgcaa taccgcagct ggcaagtgct
     6781 gcagatgaat cgtcaggctt gctcttatcc tttcgctctg cgcagaaaag gccataaatc
     6841 catccgtctt cgtgcctggt cagtctgatg tcataaacgt tcatctcggg gtctccggtt
     6901 accggcatca cgatgggccg gttcctgaag cgccagttat tgatgccgtc agggctctct
     6961 gctatggcaa aaaacgattt gacgtcgaaa ccctcggtgc gtatagccag gcagtatttg
     7021 ccatcaagct ttattgcgcc ggcattgaag gttgtattta tgccgaggcg tgtcataaga
     7081 tggggattgc ttcggtaatc gaggtcatac ctccagaaca ccggcgtatg gtcgcgggtg
     7141 attatcggat ggaggtacct atcgaagatt ccgttgccgc cttccttcct gatattgggc
     7201 ctgctgatga gtttttcatg atcatcaaaa aggcgtttta ccctgtcatc gaattcctgt
     7261 tctgtcatgg ttcccggttt tttccgaaac aaatataacg gaaaacgcaa atgacatgct
     7321 ttgattgccc tgcacgcaat gcatttgtat tttttataaa ttgcagtgat ccaaataata
     7381 ccatgactat gagaaaaa
//
LOCUS       DAOPBK010000132         7388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9067_length_7388_cov_4.527888, whole genome shotgun sequence.
ACCESSION   DAOPBK010000132 DAOPBK010000000
VERSION     DAOPBK010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9067_length_7388_cov_4.527888"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PLA17_08200"
     CDS             <1..1038
                     /locus_tag="PLA17_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK85435.1"
                     /translation="TAAIHLALVLLGVKPGDYVIGTSFTFSATVNPIAYLGATPVLVD
                     SEPGTWNMDPELLEQAIKEVSGKWEVENEKWKDKIGKLKDKREKGGGGRIKAIVVVHL
                     YGMPARMKEIMAVADRYGIPVVEDAAEALGSEYMGRRVGCNSRFGILSFNGNKIITSS
                     GGGALLSDDASLIERARFLATQARDPAPHYQHSVIGYNYRMSNVVAGVGRGQLEVLDL
                     RIKQHREFNAWYRELLKDVPGVTFQSEPSPDFCSNFWLTCIVIDPAVAGTDREKLRLA
                     FEAAGIESRPLWKPMHLQPVFAGCPAYVNGTSEKLFENGLCLPSGSNIDADGRARIAE
                     VLKREFRIVNA"
     gene            1031..2179
                     /locus_tag="PLA17_08205"
     CDS             1031..2179
                     /locus_tag="PLA17_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPK85436.1"
                     /translation="MPKKVFVSGCFDMLHSGHVAFFEEAATHGDLYVGVGSDRTINDL
                     KGRRTINGERERLYMISSLRFVKEAWINQGSGLLDFEDELRRLKPDIFFVNEDGYTPD
                     KKRLCDELGVELIVSRRVPVAGLPPRSTTELRSECRIPYRLDLAGGWLDQPYVSKHWP
                     GPVLTISIEPDLEFNDRSGMSSSTRRKAIELWHTDIPEGDPHLLARTLFCLENPPGTT
                     YVSGSQDAIGIVFPGLNRLDYPSGDYWPEKITPVHDPEVLDFIEEHLWFITLPPRAGD
                     FNVLDQTNISVEGARALAEAADGVWEALLKRDISAFGRFYRVSFEAQVAMFPLMKSAV
                     VSETIDHYRNQALGWKLAGAGGGGYLALVSEKPVENAIKIRIRRKRTA"
     gene            2334..2603
                     /locus_tag="PLA17_08210"
     CDS             2334..2603
                     /locus_tag="PLA17_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85437.1"
                     /translation="MYSPEIKAFIRQKSSLFWSVPEDKKEDITPALLVETILNYGSMD
                     DVRKLIRLMGMKEVARVFFSAKGRQELNYYPQIYHYFSLLFKKYA"
     gene            2596..3213
                     /locus_tag="PLA17_08215"
     CDS             2596..3213
                     /locus_tag="PLA17_08215"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPK85438.1"
                     /translation="MHKEILSEKQKELLPLIRQFGREYYLAGGTAVALYIGHRRSIDF
                     DLFKFSSVNHKKNIDKIADLRLPYEVIWREASQMNVTVGDVKMTFLEYPFHIVPKRKF
                     DNIIKIPELIDLAAMKAYALGRRSKWKDYVDLYFILKYYFSIDQISARASEIFDQLFS
                     EKLFRAQLNYFDDIDYSEPVDFVNTAVLEEEIRAFLSEKSLNVKL"
     gene            3400..4230
                     /locus_tag="PLA17_08220"
     CDS             3400..4230
                     /locus_tag="PLA17_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPK85439.1"
                     /translation="MNRLLIIFLLQLITGGLAFSQGVYFKLFPGYSFSVSREQMPDYL
                     THWVRVSTGTGFTYRNANLIDPEFSVASGVNLSAAAGYEVNDLLSVELRFSGFANTRK
                     QFEAAGKDLMNGETEWDFRSLSVMPAIVFGKSVNKTSLNIYAFSGIGAADLRVTTIIE
                     GDRRRFEFDRAPLFSWGFGLEFCYALSGKLSLFAEAGITSSYYYKPDRAHLVSSTLIP
                     AEDFMTYQKEFVYQDEITNISTDPYGMPDPNSPDTRLRETLRSNSLAAGIGIKITPWK
                     "
     gene            4227..5033
                     /locus_tag="PLA17_08225"
     CDS             4227..5033
                     /locus_tag="PLA17_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85440.1"
                     /translation="MKRLTLILLLVFFALRSYGQFYLQPSVGYAFNSNPEVMSTVLVN
                     DNQKSVYTMKLRYGQGLYAGLTAGYCLWDRFFIELDAKKGLLTRNSASIEEIDFREPD
                     HFSFVGFFGEIENSSPVLQFAPKAGYTVRTERLAASFSLGPNFMKTMVNHRVSTTDYY
                     VEGFSLVPHDVKAKQVFRGRMHTGLQAGLGMSWSLSQNLMVVFDFVTVFNNYGITSGE
                     ITSYEVDGEDRLEQLDDTEIDIDRDDNKLNHSHYGLHLGVRYVFGKKGAL"
     gene            5143..>5617
                     /locus_tag="PLA17_08230"
     CDS             5143..>5617
                     /locus_tag="PLA17_08230"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPK85441.1"
                     /translation="MSKVALITGVTGQDGAYLSEYLLKKGYTVHGIKRRASMFNTERI
                     DHIYQDPHLEQRNFILHYGDMTDSMNLTRIVQEVQPDEIYNLAAMSHVKVSFDTPEYT
                     ANADGIGTLRLLEAIRLLGLTKKSRIYQASTSELFGLVQEIPQRETTPFYPRSPYG"
     assembly_gap    5618..5717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5718..6455
                     /locus_tag="PLA17_08235"
     CDS             <5718..6455
                     /locus_tag="PLA17_08235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPK85442.1"
                     /translation="QEIPQRETTPFYPRSPYGAAKLYAYWITVNYREAYKMHASNGIL
                     FNHESPIRGETFVTRKVTRALSRIAMGVQEKMYMGNLSGKRDWGHAKDYIRAMYLILQ
                     QDEPSDYVIATGITTTIRDFIKMAAAELGLEVIFKGEGVDEKGYVVSMDEKRFINLVG
                     AEYLEGIRIKMREKREIVGVDKKYFRPTEVDLLIGDATKARTKLGWKPRYDLAALITD
                     MMEGDVKLVKKEVYLKKGGFYTPNYFE"
     gene            6538..>7388
                     /locus_tag="PLA17_08240"
     CDS             6538..>7388
                     /locus_tag="PLA17_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPK85443.1"
                     /translation="MLDRSSKVYVAGHLGLVGSAITAALKRKGYTNIIGRSFSELDLR
                     RQEDTEAFFAAEKPEAVILCAAKVGGIMANNTLRAEFIYENLMIEANVIHAAYLNGVK
                     KLVFLGSSCIYPRDCPQPMKEEHLLTSPLEYTNEPYAIAKIAGLKMCEAYNLRYGTDF
                     IAVMPTNLYGPNDNFNLTGGHVMPSLMRRIHLGKLLGNGDFAGIRADLARRPVDGLNG
                     SEPETEIMTLLEKQGIHKKGSQTTVSVWGTGKPFREFMYSGDMADATIFIMEKISFRD
                     LVPAGTRS"
BASE COUNT         1822 a         1692 c         2083 g         1691 t
ORIGIN      
        1 acggcggcaa ttcacctcgc gctggttctg ctcggtgtga aaccgggtga ttatgtgatt
       61 ggcacgtcat tcaccttctc cgcaacagtc aacccgatag cttacctggg ggccacgccg
      121 gtgctggttg acagtgagcc gggaacgtgg aatatggatc cggaactgct tgaacaggct
      181 ataaaagagg taagtggaaa gtgggaagtt gaaaatgaaa agtggaaaga taaaattgga
      241 aagttaaaag ataaaagaga aaagggggga gggggtagga ttaaggcgat tgtggtggtg
      301 catctgtacg ggatgccggc caggatgaag gagataatgg ctgtggcaga caggtatggt
      361 attcctgtgg ttgaggatgc cgccgaggcg ctgggctcag agtatatggg caggcgtgtg
      421 gggtgcaaca gcaggttcgg tattctgtcg ttcaacggca acaagataat cacctccagc
      481 ggcggcgggg cactgctctc ggatgatgct tcattgattg agcgtgcacg tttccttgcc
      541 acacaggctc gtgacccggc accacactac cagcacagcg tcatcgggta caactaccgc
      601 atgagcaatg tggtggcagg cgtgggacgc ggacagctcg aggtgcttga tctccgcata
      661 aaacagcatc gcgagttcaa cgcctggtac cgcgagctgc tgaaggatgt gccgggtgtc
      721 actttccagt cggaaccttc tccggatttc tgttccaact tctggctgac atgcattgtg
      781 atagatcccg ccgtggcggg aacagaccgt gagaagcttc gccttgcgtt tgaggcggca
      841 ggtattgagt cgcgccccct ctggaagccg atgcacctgc agcccgtctt cgccggctgc
      901 ccggcctatg tcaacggcac ctcggagaag ctgtttgaga atggcctctg cctgccgagc
      961 ggctccaaca ttgatgctga cggtcgtgca aggattgcag aggtgctgaa acgggaattc
     1021 agaattgtga atgcctaaga aagtatttgt cagcgggtgt tttgacatgc tccattcggg
     1081 tcatgtggcc ttctttgagg aggcagccac gcatggcgac ctgtacgtgg gcgttggttc
     1141 agaccgcact ataaatgatc tgaaaggacg gcgaacaatc aacggtgagc gcgagagact
     1201 atacatgatc agttcgctgc gctttgtgaa ggaggcatgg atcaatcagg gatcggggtt
     1261 gctcgatttt gaagatgagc ttcgcaggct gaagcctgat attttctttg tcaatgagga
     1321 tggctatacc cccgacaaga agcggctctg cgatgagctg ggggttgagc tgattgtaag
     1381 cagacgtgtt ccggtagcgg ggctgccgcc acggtcaacc acggagctgc gcagcgagtg
     1441 ccgcatacca taccggcttg acctggcagg cggttggctc gaccagccct atgtctcgaa
     1501 acactggccg gggccggtgc tgacgatatc tatcgagccc gacctggagt tcaatgaccg
     1561 cagcgggatg tcgagttcaa cccgcaggaa ggccatagag ctgtggcata cggatattcc
     1621 cgagggtgat cctcacctgc tggcacgtac gcttttctgc ctggagaatc cgcccgggac
     1681 cacttacgtc agcggctcac aggatgctat tggcatagtc tttcccggac tgaacaggct
     1741 cgattatcct tccggcgatt actggccgga gaagatcacg cccgtgcatg acccggaggt
     1801 gctcgacttc attgaagagc acctgtggtt tatcactctg cctccgcgtg ccggtgattt
     1861 caatgttctg gatcagacga atatttccgt tgagggggca cgtgcactgg cagaggcggc
     1921 agacggggtg tgggaagctt tgctaaagag agatatcagc gcgttcggga ggttttaccg
     1981 cgtttccttc gaggcgcagg tggcgatgtt cccgctgatg aagagcgctg tggtgagtga
     2041 gaccattgac cattaccgca atcaggcgct ggggtggaag ctggcaggcg ctggcggcgg
     2101 agggtacctg gcattggtat cggagaagcc ggttgagaat gcgataaaga ttcgcattcg
     2161 caggaaaaga actgcctgag aggctggaag ctggcagcca acactattat ctggcagtat
     2221 cagccggcag tgggcattat catttggcag tttctgtcgg cagtgggcag caacgggcaa
     2281 aaatggttgg ataaacgggt gattttaact atatttgggt atagccattg agtatgtata
     2341 gtccggaaat aaaggcgttt ataaggcaga aaagcagcct gttctggtct gttcccgagg
     2401 ataaaaagga ggatatcact cccgcccttc tggtggagac aattctgaat tacggctcaa
     2461 tggatgacgt cagaaaactt atcaggctga tgggtatgaa ggaggttgcc agggtattct
     2521 ttagtgcaaa gggcaggcaa gaactgaact attatcctca gatttaccac tacttctcac
     2581 ttctgttcaa aaaatatgca taaggagatc ctttcagaaa aacagaagga attgctacca
     2641 ttgatcaggc agttcggcag ggagtattat cttgcgggtg gtacagctgt agccttgtat
     2701 attggtcatc gtcggtcgat tgattttgat ctttttaaat tctcttctgt caaccataaa
     2761 aagaatatcg ataaaatagc agacttgcga ttgccctacg aagtgatttg gagggaagcg
     2821 agccagatga atgttactgt gggtgatgtc aaaatgacat ttttagagta tccttttcat
     2881 attgtaccga agaggaaatt cgacaatata attaagatac ctgaattgat agatcttgct
     2941 gcaatgaaag cctatgcact cggcaggcgc tcaaagtgga aggactatgt tgatctctat
     3001 ttcatactga aatattattt ctcaatagac cagatctcag cgagggcatc agaaatattt
     3061 gaccaattgt tctcagaaaa acttttcagg gctcaactca actatttcga tgatattgac
     3121 tattctgaac cagttgattt tgtcaacact gctgttctcg aagaggagat tcgagccttc
     3181 ctttctgaga aatcactcaa tgtcaaatta tgatttctat tgtcacccct tgctcatttc
     3241 agattactgt cacgagaatt ggcccgcaga atctgtcctt atcatccccg ggctgccgga
     3301 agcctgtccc gacagccgtc aggctgtcag gaactgtaac aatttcagaa agcaagtact
     3361 atctttaaca tattatttga aagattgaac ttcagtgcca tgaacagact cctgatcatt
     3421 ttcctgttac agctgataac gggcggactg gctttttccc agggagtata ctttaaactg
     3481 tttcccggat atagcttttc cgtctccaga gagcagatgc ctgattacct gacacactgg
     3541 gtgagggtat ctacagggac aggtttcaca tatcggaatg caaacctcat tgaccctgaa
     3601 ttctccgtcg cctccggcgt caacctcagc gctgcggcag gttacgaggt caatgacctc
     3661 ctctcggtgg agttgagatt ctccggattt gccaataccc ggaaacagtt cgaagctgcc
     3721 ggcaaagacc tgatgaacgg agagaccgaa tgggatttcc ggagcctgag cgtcatgccg
     3781 gcaatagtgt tcggcaagag tgtcaataaa acgtcactga acatctatgc cttttccggc
     3841 ataggggcag ctgacctcag ggtaacaaca ataattgaag gtgaccgccg caggtttgaa
     3901 tttgaccgtg ctcccctctt ctcctggggc tttggcctgg agttctgcta tgccctctcg
     3961 gggaaattaa gcctttttgc agaggccggc atcacaagct cgtactacta caagcctgac
     4021 agggctcacc tggtgtcatc cacactaatc ccggctgagg attttatgac ttatcagaag
     4081 gaatttgttt atcaggatga gatcaccaat atcagtacag acccttatgg catgcctgat
     4141 cccaactcac ccgacacccg gctgagggag actctcagat caaacagcct cgcggcaggc
     4201 attggaatca aaataactcc ctggaaatga aacgactgac tctcatatta ctccttgtct
     4261 tcttcgcttt gcgctcctac ggacagtttt atctccagcc ctcggtcggg tatgccttta
     4321 acagcaaccc ggaagtgatg agtacggtgc ttgtaaatga taaccagaaa tcggtttata
     4381 cgatgaagct ccgttacggg caggggctct atgccgggct gacggcagga tactgccttt
     4441 gggacaggtt ctttattgag cttgacgcga agaaggggct gctgacgcgg aacagcgcat
     4501 cgatagagga gatcgacttc cgggaaccgg atcacttctc ctttgtcgga ttcttcgggg
     4561 agatagaaaa cagcagccct gttttgcagt tcgcgccaaa ggcgggatac accgtgcgga
     4621 cagagaggct tgccgcctcg ttcagcctgg ggccgaactt catgaagacg atggtgaatc
     4681 acagggtatc cacaaccgat tattacgttg aggggttctc tcttgtcccg catgatgtaa
     4741 aggcgaagca ggtgttccgg ggcaggatgc atacggggct gcaggccggg ctgggaatga
     4801 gctggtcgtt gagtcagaat ctgatggttg tgttcgactt tgtgactgtt ttcaacaact
     4861 acggaatcac ctccggcgag atcacttcat acgaggttga cggcgaggac cggctggagc
     4921 aacttgatga cactgagata gatattgatc gtgatgataa caagctcaat cacagccatt
     4981 acggactgca cctcggggta cggtatgttt tcgggaagaa gggtgcgttg tagcctgatt
     5041 gagagtgact aagcctgcag ggccgggcgg catcgccggc tggcatgtca gcgggaagct
     5101 tttaatatcc atggattgta gtaattttac acgaaaaaca atatgtcaaa agtagcattg
     5161 atcaccggcg tgaccgggca ggacggcgca tacctgagcg aatacctcct gaaaaaggga
     5221 tacacggtac acggcataaa gcgtcgtgca tcgatgttta acaccgagag aatagaccat
     5281 atctaccagg atccgcacct ggagcagcgc aatttcatac tgcactacgg cgatatgacc
     5341 gactcgatga acctcacgcg catcgtgcag gaggtgcagc ccgatgagat atacaacctc
     5401 gcggcaatga gccatgtcaa ggtgagcttc gatacacccg aatataccgc caatgccgac
     5461 ggcataggca ctctgcggtt gctcgaggca atcaggctgc tggggctgac gaagaagtcg
     5521 cgtatctacc aggcatccac ctcggagctc ttcggcctgg tgcaggagat tccgcaacgc
     5581 gagaccactc cgttctatcc ccgttcgccg tatggcgnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnncag gagattccgc aacgcgagac cactccgttc tatccccgtt
     5761 cgccgtatgg cgcggccaag ctctacgcat actggataac cgtcaactac cgcgaggcct
     5821 ataagatgca cgcaagcaac gggatactct ttaaccatga gtcgcctatc cgaggagaga
     5881 catttgtcac aagaaaggtg acccgggcgt tgagccgcat agcaatgggt gtgcaggaga
     5941 agatgtacat ggggaacctc tcgggcaagc gcgactgggg ccatgcaaag gattatatca
     6001 gggcgatgta cctgatactg cagcaggatg agccgtcaga ttacgtgatt gccacaggga
     6061 taacaacgac aatacgtgat ttcattaaga tggctgcggc tgagctgggg ctggaggtca
     6121 tctttaaggg tgaaggggtt gatgagaagg gatatgtggt cagcatggat gagaagcgtt
     6181 tcataaatct cgtgggggcc gagtacctcg aagggataag gatcaagatg agggagaaga
     6241 gggagattgt gggtgtcgac aagaagtatt tccgtcccac agaggttgac ctgctgattg
     6301 gtgatgccac gaaagcgcgc acaaagctgg gctggaagcc gcggtatgac ctggcggccc
     6361 tgataaccga catgatggag ggagacgtga agctggtgaa gaaggaggta tatctgaaga
     6421 agggcggctt ctatactccg aattattttg agtagatatt ttgccggaag caggcaacag
     6481 acaataatgt aacatggtgt ttgcggaagc ctgcagggtt taaagatttg taaaaagatg
     6541 ttagacagga gttccaaagt atatgtggcc gggcacctgg gactggttgg ttcggcgata
     6601 acggcagcgc tgaaaaggaa gggatatacc aatattatcg gacggtcgtt cagcgagctc
     6661 gatctgcgcc ggcaggagga tacagaagca ttttttgctg ccgaaaaacc ggaggcagtg
     6721 atactttgtg cagccaaggt aggaggcatc atggccaaca acaccctcag ggcagagttc
     6781 atctatgaga acctgatgat agaggccaat gtaatacatg cggcctatct gaatggcgta
     6841 aagaagctgg tattcctggg aagcagctgc atctacccgc gcgattgccc ccagccgatg
     6901 aaggaggagc atctgctaac ctcgccgctg gagtacacca acgagccgta tgccatagcc
     6961 aagatcgcag gactgaagat gtgcgaagcg tacaacctgc gatatggcac tgacttcata
     7021 gcagtgatgc ccaccaacct ctatggaccc aatgacaact ttaatcttac cggtggtcat
     7081 gtgatgccgt cgcttatgag acggattcat ctcggcaagt tgctgggtaa tggtgacttt
     7141 gccggcatca gggccgatct ggccagaagg cctgttgacg gtttgaatgg cagcgagcca
     7201 gagacagaaa taatgacgct gcttgaaaaa caggggatac acaaaaaggg ctcgcagact
     7261 acagtttctg tatggggaac gggcaaaccg ttccgcgagt tcatgtacag cggtgacatg
     7321 gccgatgcaa ccatcttcat catggagaag atctcgttca gggacctggt tcctgccgga
     7381 acgagatc
//
LOCUS       DAOPBK010000133         7281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9209_length_7281_cov_4.432328, whole genome shotgun sequence.
ACCESSION   DAOPBK010000133 DAOPBK010000000
VERSION     DAOPBK010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9209_length_7281_cov_4.432328"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3399)
                     /gene="ileS"
                     /locus_tag="PLA17_08245"
     CDS             complement(<1..3399)
                     /gene="ileS"
                     /locus_tag="PLA17_08245"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPK85444.1"
                     /translation="MSKKFPEYKGLDLPKVNDEVLKMWDNHDTFRKSLRSRDGKGEFV
                     FYEGPPSANGMPGIHHVMSRAIKDTICRFRTLQGYEVKRKAGWDTHGLPVELGVEKTL
                     GITKEDIGTNISIEDYNKACRKDVMKFTDVWENLTRHMGFWIDMDNPYITYDNRYIET
                     VWWLLKQLYNKGLLYEGYTIQPYSPAAGTGLSSHELNMPGCYKDVRDTTCIALFSLIR
                     NEKSEFLFRGIDTDGVKIMAWTTTPWTLSSNTALAVGPDISYVRVRTTNPYTSEPITV
                     ILAEDLLSVIFTPDKKLQYTVTGKYKGADLKGIDYEQLIDWVKPDEGAFRVITGDFVT
                     TVDGTGIVHIAPTFGADDYRAGREHGIPPLMVKMSDGFMGPLVDRRGRMVPLEDLDQD
                     FVASRVNKETYGPFAGRYVKNEYDETIPANSDTLDVDIAVTLKQQGKVFRIEKQVHNY
                     PHCWRTDKPVLYYPLDSWFIRSTALRDRMIELNNTINWKPESTGTGRFGKWLENLVDW
                     NLSRSRYWGTPLPIWMTEDRQEAICIGSLAELKREMARSVDAGYMTSNPLQDFAEGDF
                     STENYSLLDLHRPYVDDIVLVSPTGRPMHREPDLIDVWFDSGAMPYAQAHYPFENREN
                     FDEMFPADFIAEGVDQTRGWFFTLHAIATMISDSVSFKNIISNGLVLDKNGNKMSKRL
                     GNAVDPFLIMDKHGADSLRWYMLTNAQPWDNLRFDTSGVEEVKRKFFGTLYNTYSFFA
                     LYANVDGFTGREPQVPKERRPEIDLWIISLLNSLVKEAGERYDDYDITPAGRAISDFV
                     TENLSNWYVRLNRKRYWGGGMDEDKLSAYQTLYTCLETVALLASPIIPFYTDRIFTDL
                     NSVSSRYSEGSVHLAPFPSYDEKLIDKDLEERMEIAQKMSSMILALRRKVNIRVRQPL
                     AKIMVPVTGPHFRQMFEAVKPLILAEVNVKDVEYVDDTSGILVRRIKPNFKLLGPKFG
                     KQMKEAGAAIMALTQQEIVQMERDGFLDLSIGGASHRITAEEVEIISEDIPGWLVANE
                     GRLTVALDITVTPELLHEGIAREFVNRIQNIRKESGFEVTDKIRVVIEDLPFVAEAVQ
                     KHSDYIASQTLALEVSLAPGSGLTGPAARETDIEEQMVKVLVEKV"
     gene            3806..4951
                     /locus_tag="PLA17_08250"
     CDS             3806..4951
                     /locus_tag="PLA17_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK85445.1"
                     /translation="MKEKRNFIQVIAGPRQVGKTTLVGQLLERLNIPSHYVSADSAGP
                     QGALWIEKHWEAARRMLISEGAREIILVIDEIQKLENWSEMVKKLWDEDTWTRTPLKL
                     IISGSSRLLLQEGLTESLGGRFEMTLMGHWTLKEMQEAFGWSPEQYVWYGGYPGSAVI
                     VDDEMRWKRYLADSIVETTISRDILMLTRVDKPALLRRLFETGVRYSGMELSYNKILG
                     QLQDAGNTTTLSHYLTLLGSAGLLSGIEKYSGSFARQRASSPKFMVHNTALMSSADNE
                     SFSSIREQPDRWGRYVESAVGAHLLDGSMKGHYRLFYWRKGNREVDFVLARGEKIIAM
                     EVKSGRIRGRSGLDDFRKEYPHASVMLVGDKGIPWQTFLRTDTAGII"
     gene            5073..5450
                     /locus_tag="PLA17_08255"
     CDS             5073..5450
                     /locus_tag="PLA17_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPK85446.1"
                     /translation="MAEKTRYTDEELEEFRQIIMAKLEKARKDYDVLKASISHEDNND
                     TMDTSPTFKVLEEGATTLSKEEAGRLAQRQQKFIHHLQAALVRIENKTYGICRETGKL
                     IGKERLRAVPHATLSMEAKLKQK"
     gene            5584..5859
                     /locus_tag="PLA17_08260"
     CDS             5584..5859
                     /locus_tag="PLA17_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85447.1"
                     /translation="MHRQNLRITRTSAPPESPHRQELRTIRNFALSGTPHYQDFCTIR
                     TSAPSGTQHHQNLRTISTSALSEPPHYQDLYTTRASTLSNSTLTARA"
     gene            complement(5837..>7281)
                     /locus_tag="PLA17_08265"
     CDS             complement(5837..>7281)
                     /locus_tag="PLA17_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPK85448.1"
                     /translation="SMLKPAGLKTYMLSHITFFLVLALLWIVVSVVSGKIGRGRIINL
                     RTLYARVLTANLIATSMAALLLFALRDLGYSRQVVFGTAITATVLELVLGSLYFAFRK
                     APLITPAEIKLSERELVARTHPGEASDIIPDPSLTIRLQEGCSRVRAEAFSSLVNRED
                     VARLAIVSTSEAFNIQNLEHDQYSCIINLKPMNNIRKLDRFLDIVNGRLGGGGLFLCS
                     VETLEQRAGRLKEKYSPFIYYLIYLPDFLINRVIPRLRLTRGLWEFIARGANPPFSRA
                     EALGRLSRAGFAIKPERFAGNMLCIKAERISDPKPVNENGYGMIISLPRIGRDGRTFN
                     VYKLRTMHPYSEYIQDYVYGLHSIADGGKMKHDFRVTAWGQFARKVWLDELPMIVNIL
                     KGDIKLFGVRPLSKHYFSLYDDEIRNRRIRYKPGLLPPYYADLPTGLEAIQHSELKYF
                     DSWDQNPIGTDIRYLTRSIYNIFFRHARSA"
BASE COUNT         1737 a         1873 c         1981 g         1690 t
ORIGIN      
        1 aaccttctcc accagcacct ttaccatctg ctcctcgatg tcagtctcgc gggcagcggg
       61 gccggtgaga ccggaaccgg gagccaggga gacctccaga gccagcgtct gcgaggcaat
      121 ataatccgag tgcttctgta cagcctctgc cacaaagggc agatcctcaa tcacaaccct
      181 gatcttgtca gtcacctcaa acccgctctc cttgcggatg ttctggatgc ggttgacaaa
      241 ctcgcgggca ataccctcgt gaagcagctc cggggtgacg gtgatgtcca gagccacggt
      301 cagcctaccc tcgttggcaa caagccatcc gggtatatcc tcggagatga tctccacctc
      361 ttcagcagtg atgcggtgcg atgcgccacc aattgaaaga tcgaggaaac cgtcgcgctc
      421 catctgcaca atctcctgct gcgtcagcgc catgatggca gcaccggcct ccttcatctg
      481 ctttccgaac ttcgggccca gcagcttgaa gttgggcttg atgcgacgca caagaatacc
      541 cgatgtgtca tctacatact ccacatcctt gacgttgacc tcggcaagta tcagaggctt
      601 taccgcctcg aacatctggc ggaagtgcgg accggtcacc ggcaccatga tctttgccag
      661 cggctggcgc accctgatgt tcaccttgcg gcgcagagcc agtatcatgg acgacatctt
      721 ctgggcaatc tccatccgct cctcaagatc cttgtctatc agcttctcat catacgacgg
      781 gaagggcgcc aggtgaacag agccctcaga gtagcggctg ctaacagagt tgagatcagt
      841 aaagatgcgg tcagtataga acggtattat cggcgaggcc agcagagcca ccgtctcaag
      901 gcatgtatag agcgtctggt atgcagagag cttgtcctcg tccatgccgc cgccccagta
      961 acgcttgcgg ttgaggcgca cgtaccagtt ggagagattc tcggtcacaa aatcagagat
     1021 ggcacgcccc gccggggtga tgtcatagtc gtcataacgc tcacccgcct ctttaaccag
     1081 cgagttgagc agcgagatta tccagaggtc tatctcgggc cggcgctcct tgggtacctg
     1141 cggctcacgg ccggtgaacc cgtcaacatt ggcgtaaagc gcgaagaatg aatatgtatt
     1201 gtagagtgtc ccgaagaatt tgcgcttcac ctcctccaca ccagatgtgt caaaacgaag
     1261 gttatcccac ggctgggcgt tggtgagcat gtaccagcgg agcgaatcag cgccatgctt
     1321 gtccattatc aggaagggat cgacggcgtt gccgaggcgc ttcgacatct tgttgccgtt
     1381 cttgtcgagc accaacccgt tggatatgat gtttttaaac gaaaccgaat cagagatcat
     1441 tgtggcgatg gcgtgcagcg tgaagaacca cccgcgcgtc tggtcaaccc cctcggcaat
     1501 gaaatcggcc gggaacattt catcaaagtt ctccctgttt tcaaaaggat agtgcgcctg
     1561 tgcatagggc atcgcccccg agtcaaacca tacgtctatc aagtcaggct cacggtgcat
     1621 gggcctgcca gttggactca ccagcacaat atcatccaca tacggcctgt gcaggtcgag
     1681 cagtgagtag ttttccgtgg agaagtcgcc ctcggcgaaa tcctgcagcg ggttgctggt
     1741 catataaccg gcatcgacac tgcgtgccat ctccctcttc agctccgcga gcgagccgat
     1801 gcagatcgcc tcctggcggt cttcagtcat ccagatgggg agtggtgtgc cccagtacct
     1861 tgagcgcgac aggttccagt cgacgaggtt ctcgagccac ttgccgaagc ggcctgtacc
     1921 ggtactctcc ggcttccagt tgatggtgtt gttcagctca atcattctgt cacgcagagc
     1981 ggttgagcgt atgaaccacg agtcaagcgg atagtagagc acaggcttgt cagtacgcca
     2041 gcagtgcggg tagttgtgca cctgcttctc tatgcggaac accttgccct gctgcttgag
     2101 cgtcacggca atgtctacat cgagggtatc gctgttggcg ggaatggtct cgtcatattc
     2161 attcttgaca tagcgtcccg cgaagggtcc gtaagtctct ttgttaacac gtgatgccac
     2221 gaagtcctgg tcgaggtcct ccagcggcac catgcgaccg cggcggtcga cgagcggacc
     2281 catgaagccg tcagacatct tcaccatgag cggcgggatg ccgtgttcgc gcccggcgcg
     2341 atagtcgtcg gctccaaagg tgggagctat atgaacaatt cctgttccgt cgacggtggt
     2401 gacaaaatcg ccggtgatga cgcggaaggc gccttcatca ggcttcaccc agtcgataag
     2461 ctgctcgtag tcaatccctt tgagatcagc acccttgtat ttcccggtaa cggtgtattg
     2521 caattttttg tcgggagtga agatcacgga aagcaaatcc tctgcaagta tcactgtgat
     2581 gggctccgag gtgtagggat tggtggtgcg gacgcgtaca taggagatgt ctggtccaac
     2641 ggccagcgcg gtgtttgaag agagtgtcca gggtgtggtg gtccacgcca tgatcttcac
     2701 gccgtcagtg tcaattcccc tgaagagaaa ctctgatttc tcattgcgga tcaggctgaa
     2761 gagggcgatg caggttgtgt ccctgacgtc cttgtagcat cccggcatgt tgagctcgtg
     2821 cgagctgagg ccggtgccgg cggcgggaga gtagggctgg attgtataac cctcgtagag
     2881 cagacccttg ttatagagtt gtttgagcag ccaccagact gtctcgatat acctgttgtc
     2941 ataggtgatg taggggttgt ccatatcgat ccagaacccc atgtggcggg tcaggttttc
     3001 ccatacatcg gtaaacttca tcacatcctt gcgacaggcc ttgttgtaat cttcaatgga
     3061 aatatttgtt ccgatgtctt ccttggttat cccgagagtc ttctccacgc ccagttcaac
     3121 aggcagtccg tgggtatccc agccggcctt gcgtttgacc tcataaccct gcagtgtacg
     3181 gaaacggcag atggtatcct tgatggcgcg cgacatgaca tggtggatgc cgggcatccc
     3241 gttggctgag ggaggaccct cgtagaagac gaactcacct ttgccgtcac gactgcgcag
     3301 cgacttccgg aaggtatcat ggttatccca catcttcagc acctcgtcgt tcaccttcgg
     3361 gagatcaagg cccttgtact cgggaaattt ctttgacata cggttttacc tgttaaaatc
     3421 agcgtgcaaa gttagtaaaa gttggagaaa tggcagcagg caaaagcatt agtggatttc
     3481 gtagcaggct ggaacgaatt tgcgatctgc gatttgtgat tgctggtttt gccccatccg
     3541 cagggagtca ggccatcagg aactgccgat ttgcgattct ggctggcaat ttataactgt
     3601 gaccggaatg atctccctga ctccccctca gtcaggctaa ccattagggc acagagacat
     3661 ggctgcagat aacaattttt agtgagaaag ccgcactcaa ttattatttg tttttactag
     3721 ttatattgag taaatttact cagcatattg agtaaaaatt aatagcatgt ttcaaagatc
     3781 agaaatagag caacttgagt cccggatgaa agagaaaaga aacttcatcc aggtcattgc
     3841 cggtccaagg caggtaggca aaacgactct cgtgggtcaa ttgcttgaga ggctgaatat
     3901 accttctcat tatgtctctg ctgacagtgc ggggcctcag ggtgcgttat ggatagagaa
     3961 acactgggaa gcagcaaggc ggatgctgat tagtgagggc gcccgcgaaa taattctggt
     4021 cattgatgaa atccagaagc ttgaaaactg gagtgagatg gtaaagaagc tgtgggatga
     4081 ggatacctgg accaggactc ccctgaaact cattatttca ggatcatcac gacttcttct
     4141 tcaggaagga cttacagagt ctctcggggg aaggtttgag atgacgctca tgggtcactg
     4201 gacacttaag gagatgcagg aggcttttgg ctggagtccg gagcagtatg tctggtatgg
     4261 cggatacccc ggctctgcag taatagttga tgatgagatg agatggaaaa gatacctggc
     4321 cgattctata gttgaaacaa ccatctcgcg tgacatcctt atgctcacac gggtagataa
     4381 gccggcattg cttcgccggt tatttgagac gggcgtcagg tattccggga tggaactctc
     4441 ttataacaag attcttggtc agctgcagga tgccggaaat acaactactt tgtcccatta
     4501 cctgacgctt ctcgggtctg caggattatt atcaggaatt gaaaagtatt ccgggagttt
     4561 tgccagacaa cgggcatcaa gtcctaagtt catggtacac aatacagccc tgatgagctc
     4621 ggcagataat gaatctttca gcagtatcag ggaacagccc gacaggtggg gaaggtatgt
     4681 ggaatctgca gtgggggctc atttgctcga tggcagcatg aaaggtcatt acaggctctt
     4741 ttactggagg aaaggcaaca gggaggtcga ttttgtcctt gccaggggag agaagataat
     4801 tgcgatggaa gtgaaaagcg gaagaattcg cggcagatcc gggcttgatg atttcaggaa
     4861 agaatatcct catgccagtg tgatgcttgt tggtgacaag ggaattcctt ggcagacatt
     4921 cctcagaacg gacacagccg gaataatctg aatatattaa ttatcagcgc ttcaccagtg
     4981 cttcctgccc aatgcctcct gaggtacaat attcatacag ataatatgtt attttatatt
     5041 acatttgcgg aaaaatagta acacttgtag ccatggcaga gaagacaaga tatacggatg
     5101 aggagctgga ggagttcagg cagataatca tggccaagct cgagaaagca aggaaggatt
     5161 atgatgtgct caaggcaagc atatcgcacg aggacaataa tgatacaatg gacacctcgc
     5221 ccaccttcaa ggtgctggaa gagggtgcca ccaccctctc caaggaggag gccggcaggc
     5281 tggcacaacg gcagcagaag ttcattcatc atctgcaggc tgcgctggtt cgcatagaga
     5341 acaagaccta cggtatatgc cgtgagacgg gtaagcttat cggcaaggag cgcctgcgag
     5401 cagtacccca tgccaccctc tcaatggagg caaaactgaa gcagaaataa cctcagggtc
     5461 agtcgtaaga cctgatccgc cgccatgctg gaaaggatct taaggtcatg gattatccgt
     5521 gacctttttt agtctgatgg agtcagctgt tagtatagct gtcagatttc agggatcaaa
     5581 tttttgcatc gacagaatct acgcatcacc aggacttctg caccaccaga atctccgcat
     5641 cgtcaggaac ttcgcactat caggaacttc gcactatcag gaactccgca ctaccaggac
     5701 ttctgcacca tcaggacttc tgcaccatca ggaacacagc atcaccagaa tctacgcacc
     5761 atcagcacct ctgcactgtc agagcctccg cactatcagg atctctacac caccagagcc
     5821 tccacactat caaactctac gctgaccgcg cgtgcctgaa gaagatattg taaatactgc
     5881 gggtgaggta acggatgtca gtccctatcg gattctggtc ccatgaatcg aaatacttca
     5941 gctccgagtg ctgaatagct tcgaggccgg tgggcagatc ggcatagtat ggcggcagca
     6001 ggcccggctt gtaccttatg cggcggttgc ggatctcatc gtcatagagg ctgaagtagt
     6061 gtttcgagag tggccttacg ccgaacagct tgatatcgcc cttgagtata ttcactatca
     6121 ttggcagctc atcaagccat accttccttg caaattgtcc ccacgcggtc acgcggaagt
     6181 catgcttcat cttcccgccg tcggcaatag agtgcagtcc gtaaacataa tcctggatat
     6241 actccgagta gggatgcatc gtgcgaagct tgtaaacatt gaaggtgcgc ccgtcacgac
     6301 cgatcctggg gagtgatatt atcatcccgt aaccgttctc gttgaccggc ttgggatcag
     6361 agatacgctc tgccttgata cagagcatgt ttcctgcaaa tctctccggt ttgatcgcaa
     6421 accctgcacg actcaggcgg cccagtgcct cggccctgga gaagggagga ttggcgccgc
     6481 gggcgatgaa ctcccagaga ccccgggtga gccgcagtcg cggaataaca cggttgataa
     6541 gaaaatcggg caggtagatc aggtaataaa taaatggtga atacttctcc ttcaaccgcc
     6601 cggccctctg ttccagtgtc tccaccgagc agaggaagag acctccgccc cccagcctgc
     6661 cattgacaat atcaagaaac ctgtcgagct tcctgatatt gttcatcggc ttgaggttga
     6721 taatgcagga gtactggtcg tgctcaaggt tctgaatatt aaatgcttcc gaggttgaca
     6781 ctattgccag tcgtgccaca tcttccctgt ttaccagtga cgagaacgcc tcagcgcgga
     6841 cccttgagca cccctcctgg agccttattg tcagcgacgg atcagggatg atgtcgcttg
     6901 cttcaccagg atgggtgcgg gctacgagct cacgctcgga aagctttatc tctgccgggg
     6961 tgatcagcgg cgccttgcgg aaggcgaagt atagtgagcc aagcaccagc tcaaggacgg
     7021 ttgcggttat ggcagtgccg aagaccacct gccttgaata acccaggtcg cgaagggcga
     7081 agagcagcag ggctgccata cttgtggcta tcaggttggc tgtcagtacc cgcgcgtaga
     7141 gtgtgcgaag gttgattatt cggccgcggc ctatcttccc gctgacaacg gagaccacaa
     7201 tccacagtag cgccagcacc aggaagaatg tgatatgcga gagcatgtag gttttcaggc
     7261 cggccggctt gagcattgac a
//
LOCUS       DAOPBK010000134         7276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9215_length_7276_cov_5.145825, whole genome shotgun sequence.
ACCESSION   DAOPBK010000134 DAOPBK010000000
VERSION     DAOPBK010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9215_length_7276_cov_5.145825"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLA17_08270"
     CDS             <1..486
                     /locus_tag="PLA17_08270"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPK85449.1"
                     /translation="TGIGMSQRMQSFVLDEKENPFNVVEPGKRPRVTLTPTLAMKEGK
                     PLLSFAKQGGDEQDQLLLQFFLNVVEFGMTIQEACEAPSFKTLQMYSSFGDHEKTPGG
                     LVLNSATPQWIRKELAAMGYRLSFQERTSGPVNAIGFDHAHGTFWGGSSNHGEDYGIG
                     W"
     gene            614..1492
                     /locus_tag="PLA17_08275"
     CDS             614..1492
                     /locus_tag="PLA17_08275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPK85450.1"
                     /translation="MAKNEKRKGKWSDRFRFAVFNDTTFEELWRIRLSKANALLFVAV
                     LLVVTLALNTSLIAFTNLREFIPGYPDVEMRRDILMNAIMLDSLKHELEIRDKYFRDL
                     NDVISGRQPASSVTLRDSTRDYSNIVFRKSYEDSLFKARVEQEEKYSLSASAMSPATP
                     STVQGTTLANIHFFPPVKGIISSNFDTRTRHYATDIVTQPKAVVSSTLDGTVIMTGWT
                     METGFVIMVQHANNLISVYKHNARLLKETGDRVRAGEPISIVGDSGELYTSGPHLHFE
                     LWHNGVALDPAQYIYF"
     gene            1507..2673
                     /locus_tag="PLA17_08280"
     CDS             1507..2673
                     /locus_tag="PLA17_08280"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPK85451.1"
                     /translation="MHKRLAIIGSTGSIGTQTLEIVACYPDLFTVTALTAGNNAGLLT
                     EQARLFRPEKVVIGNEAYYEVVKSGLSGLGIEVMAGQKAVEEVASSDGIDTVVAAMVG
                     FAGLRSTAAAVAAGREVALANKETLVVAGEMITRTAAITGTRLLPIDSEHSAIMQCLN
                     GEPESSVERIILTASGGPFRDTPAERLAKVTPQEALRHPNWTMGNKITIDSATLMNKG
                     LETIEAHWLFGTPPERIDVLIHPQSIIHSMVQFTDGSLKAQLGVPDMRLPIIYALTWP
                     DRVVTELPRPDLTETGSLTFGEPDTARFRCLALARAAMKEGGNMPCALNAANEVAVAA
                     FLREQIGFNDIAGTVEYVLGKTTFNSGASLGTYAETDNASRRLAEDYINKVLKK"
     gene            2670..3995
                     /gene="rseP"
                     /locus_tag="PLA17_08285"
     CDS             2670..3995
                     /gene="rseP"
                     /locus_tag="PLA17_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPK85452.1"
                     /translation="MTVLIKILQLLLSLSILVIVHEFGHFLFARLFKTRVEKFYLFFD
                     WPRAIFRKKIGETEYGIGMLPLGGYVKISGMIDESMDREQMKQPPQPYEFRSKKAWQR
                     LMIMLGGVFFNFIFALLIYVCVLSLWGETYLATKNVKYGILTDSTGYAMGLRNGDKII
                     SVDGKEVENFYAIIPDILLNERKVITVDRDGETIDIPVLREFIPVLLKNPDIIDARIP
                     FGPFVVSGVVGESPAAAAGLAPGDEIVAIDGNPFIWYDEFQAYLAQHKAIPITVSVKR
                     EGELKDIAVTPTGAGIIGVYRETANIFELSTKHYNFFEAIPAGISKGWKTTGDYLKQF
                     KLIFAKDTKGYESLGGFITIGSIFPGKWNWQSFWNLTAFLSLILAVMNLLPIPALDGG
                     HVMFLVFEVVTGRKPSDRFMEYAQITGMIIIFALLIFANGNDILKLIRK"
     gene            4066..4239
                     /gene="tatA"
                     /locus_tag="PLA17_08290"
     CDS             4066..4239
                     /gene="tatA"
                     /locus_tag="PLA17_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPK85453.1"
                     /translation="MGRIGPLEIVLIILVVVLMFGGRKIPELMKGIGQGLKEFKKARE
                     TGDDADKDAAKEN"
     gene            4305..5288
                     /locus_tag="PLA17_08295"
     CDS             4305..5288
                     /locus_tag="PLA17_08295"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HPK85454.1"
                     /translation="MRKASVFLFIMVAFIASCGDSSLTRTLPNVTGTTGEVMVVMDKF
                     MWDGSPGKAIREQVSPLLVGLPQPEPAFNILPVNPSGFRDIYVSHRNIIQVTIDPGIQ
                     KPAVTYTRNKWAETQLVVNISAADTASLAAAVRENSDEIRRSINDAERERLITWLTNS
                     AGKHRSVLNTGEARWELAIPAGWKPDFNRDDIMMLSLETPSTTQSVMVSVTGRTTPRI
                     GCFELADLTQKRMSAEVKGPDGGSFMVIEQRMPVSCRSFRRYDTDYIEMRGLWTLEGG
                     FMGGPFISYAFIDSATARAVIVTGFVYAPKEEKRELLRQVEALMYTVKRQQ"
     gene            complement(5412..6506)
                     /gene="mqnC"
                     /locus_tag="PLA17_08300"
     CDS             complement(5412..6506)
                     /gene="mqnC"
                     /locus_tag="PLA17_08300"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HPK85455.1"
                     /translation="MGFREQIYYRLDGGGRLTVDEALNLWNEAPPEELMYVADLTRKR
                     LHPGNRAGWIIDRNVNITNICFSQCRFCNFCRTEKHEDAYVTTDEEYDRKIMELMALG
                     GDQLLLQGGMNPVLGLDFYTGLFRSLKSRWPGLKLHALGPPEVAWLARQGKMSYHDVL
                     VSLRDAGLDSLPGAGAEILSDRVRKLVSPAKATVKEWLGVMREAHRLNLPTSATMMFG
                     HAETIRERLEHLDMLRKLQDDRPAGHDGFITFIPWPFMDRGTRLAEKDGIRSRVTAPE
                     YLKLIAISRLMLDNIPNIQSSILTVGRETAMISLHCGANDLGSVMIEENVVSAAGDPF
                     RVSPGEMQQIIREAGFIPVRRDQRYIPVEE"
     gene            6634..>7276
                     /locus_tag="PLA17_08305"
     CDS             6634..>7276
                     /locus_tag="PLA17_08305"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPK85456.1"
                     /translation="MNKTPIVVGITQGDMNGIGYEVIMKALSDSEITGFCVPVVYGSP
                     KVAAYHRKVLNITNLNFNNIRSVAEAHPKKVNIINCLGDDVHVELGKSTREGGQSSLA
                     ALEAAVTDLTAGRIDVMVTAPIDKSNIQSGSFRFNGHTDWLKSKTGSHDALMLMISEA
                     MKVGFVTEHLPLKDVPGAITIGNILAKLRLLNSSLLQDFAVRGPRIGVMGLNPH"
BASE COUNT         1817 a         1788 c         1933 g         1738 t
ORIGIN      
        1 accggcatcg ggatgagcca gaggatgcag agcttcgtcc ttgacgagaa ggagaatccg
       61 ttcaatgtgg tggaacccgg aaagagacca cgtgtgacac tgaccccgac actggccatg
      121 aaggagggaa agccgcttct ctcatttgcc aagcagggtg gcgatgagca ggatcagttg
      181 ctgcttcagt ttttcctgaa tgtggtggag tttggcatga ccattcagga agcatgcgag
      241 gcaccctctt tcaagacact gcagatgtat tccagcttcg gtgaccatga gaagaccccg
      301 ggcggattgg tgctaaacag cgccacacct cagtggataa gaaaagaact ggcagccatg
      361 gggtacaggc tctcattcca ggagagaacc tccggtcctg tcaatgctat cgggtttgac
      421 catgcacacg gaaccttctg gggcggatca agcaatcacg gcgaggacta cggcatagga
      481 tggtaaacca gttggcagtg acggcaggca gccggcagta agtaattgcc gggtatcaac
      541 tgcggagtgc tgacttttca aatagaattt tccaacatca ttcttatctt tgtgccttcg
      601 gtcagattat cagatggcaa agaacgaaaa aaggaagggc aaatggagtg acaggttccg
      661 ctttgcagtt ttcaatgaca caaccttcga ggagctgtgg cggatacgac tttcgaaagc
      721 caacgcactg cttttcgtag ctgtgttgct ggttgtaact ctcgcactta acacttcatt
      781 gattgcattc accaatctca gggaattcat cccgggttat cctgatgtgg agatgcggcg
      841 ggacatcctc atgaatgcca tcatgctcga ctccctgaag catgaactcg agatcaggga
      901 caagtatttc cgtgacctta atgacgtcat ctcaggcagg cagcctgcca gctcggtgac
      961 tctgcgtgac agtacgcgcg actacagtaa tatagtgttc agaaaatcct atgaagattc
     1021 cctgttcaaa gcacgcgtag agcaggagga aaaatatagt ctgtcggcat cggccatgtc
     1081 acctgcaacc ccttcaacag ttcagggcac cactcttgcc aacattcatt tctttccccc
     1141 ggtcaagggg atcatctcga gtaattttga cacgcgtacc aggcattatg ccactgacat
     1201 agttacccag cccaaggcgg ttgtctcatc tactcttgac ggaaccgtga taatgacggg
     1261 atggaccatg gagaccggtt ttgtgataat ggttcagcat gccaacaacc tgatatcagt
     1321 ttacaagcac aacgccaggc tgctgaagga gacgggtgac agggtcagag caggagagcc
     1381 gatatcgata gtgggagatt caggtgagct ttacacttcg gggccacatc ttcattttga
     1441 gctatggcat aatggagtgg cgcttgaccc ggctcagtat atctatttct gacaggaggc
     1501 tgacggatgc ataaacgact ggcaataatc ggctctaccg gatctatagg tacacagacc
     1561 ctggaaatag tggcatgtta ccctgatctc ttcactgtta cagccctgac tgcaggcaat
     1621 aatgccgggc tgctgacaga acaggccagg ctcttccggc ctgagaaggt tgtaataggc
     1681 aacgaggctt attatgaagt tgtgaagagc ggtctgtcag gcctgggtat agaggtgatg
     1741 gctggtcaga aggctgttga ggaggttgca tcatctgacg gcatcgatac cgtggttgct
     1801 gccatggtgg gttttgccgg actgcgttcc actgccgcag ccgtggctgc aggcagggag
     1861 gtggcgctgg caaacaagga gaccctggtt gttgccggag agatgattac acgaacagca
     1921 gccattaccg gcaccagact actcccgata gattcagagc actcagccat catgcagtgc
     1981 cttaacggtg agccggagtc atcagttgaa cgtattattt taaccgcgtc aggcgggcct
     2041 ttccgtgata ctcctgctga gcggctggca aaagtcaccc cgcaggaggc gctgcgccat
     2101 cccaactgga caatgggcaa caaaatcact attgattcag caaccctgat gaacaaggga
     2161 cttgagacaa tagaagcaca ctggctcttc ggcactccgc ctgaacgtat tgatgtgctg
     2221 atacacccgc agtcaattat ccactcaatg gtgcagttca ctgatgggtc tctcaaggca
     2281 cagctcggag tgcctgacat gcggctgcct atcatctacg cactcacctg gccggataga
     2341 gtggttactg aacttccccg tcctgacctg accgagacag ggtcgctcac cttcggagag
     2401 cctgatactg ccaggttcag atgccttgcc ctggcacggg cagccatgaa agagggcggg
     2461 aacatgccat gtgccctcaa tgccgccaac gaagtggccg tggcagcatt cctccgtgaa
     2521 cagataggat tcaatgatat agccggcaca gtggaatatg tgctcgggaa aacaacattt
     2581 aacagcgggg cttctctcgg cacctatgcc gaaaccgaca atgcaagccg gcgcctcgca
     2641 gaagattata taaataaagt actgaaaaaa tgacagttct gatcaaaata ctgcagctgt
     2701 tgctgtcact gtcaatcctg gttattgtcc atgagtttgg ccacttcctt ttcgcccgcc
     2761 tttttaagac aagggtggag aagttctacc tcttctttga ctggccacgg gcgatcttca
     2821 ggaaaaagat cggtgaaacg gaatatggta tcgggatgtt accccttggc gggtatgtca
     2881 agatctccgg aatgatagat gaaagcatgg accgcgagca gatgaaacag cctccccagc
     2941 catatgagtt cagatcaaag aaagcatggc agagacttat gatcatgctt ggcggggtat
     3001 tcttcaattt catctttgca ctgctgatct atgtttgcgt actgagcctg tggggtgaga
     3061 cttatctggc aacaaagaat gtcaaatacg gtatacttac cgattctacc ggctatgcaa
     3121 tgggactgcg caacggagat aagataattt ccgttgacgg gaaagaggtt gagaactttt
     3181 atgctatcat acctgacatt cttttgaacg agcgtaaggt gattactgtt gaccgcgacg
     3241 gcgagacaat tgatatcccg gtcctgcgtg agttcatccc ggttcttctc aagaatccgg
     3301 atattattga tgcgagaatc cccttcggtc cgtttgtggt ttcaggggtt gtcggcgagt
     3361 cgcctgccgc agcagccggc ctggcaccgg gtgacgagat agttgccatt gacggcaatc
     3421 ccttcatctg gtatgatgag ttccaggcct acctggcgca gcataaagcc ataccgatca
     3481 cagtttctgt aaaaagggaa ggggaactca aagatattgc agttacacca accggggcgg
     3541 ggattatcgg tgtttaccgc gagacagcca atatttttga gctgagcaca aaacactata
     3601 atttctttga ggctatccct gccggtatct caaaaggctg gaaaacaacg ggtgattacc
     3661 tgaagcaatt caagctgatt tttgcgaagg ataccaaagg atacgaatcg ctcggcggat
     3721 tcataaccat cggaagcatc ttcccgggaa aatggaactg gcagtcgttc tggaacctga
     3781 ccgctttcct ttcactgatt ctggccgtaa tgaacctgct gccaatccct gcccttgacg
     3841 gaggccatgt gatgttcctt gttttcgagg tggtcaccgg aagaaaacca agcgacagat
     3901 tcatggagta tgcccagatc accgggatga tcataatttt tgccctgctt atttttgcca
     3961 atggtaatga tattctgaaa ctgatcagaa aatgataata ttacagttaa gtgaataacg
     4021 gaagcgaaag aatttgtaca tttgcataaa taaatcaaca gaaaaatggg aaggatcgga
     4081 cctcttgaaa tagttctgat aattttggta gtagttctga tgtttggcgg acggaagatt
     4141 cctgagctga tgaagggaat agggcagggg cttaaggagt ttaaaaaggc cagggaaacc
     4201 ggtgatgatg ctgataagga tgctgcaaag gaaaactagg ataataaaag cttatattta
     4261 agcctgcatg aaagtgcagg ctttttattt cataatagcc agagatgaga aaagcttcag
     4321 ttttcctgtt tataatggtg gcattcattg cttcgtgcgg tgactcgtca cttacaagga
     4381 cccttccgaa tgtgaccggc actaccgggg aggttatggt ggttatggac aaattcatgt
     4441 gggatggcag cccgggcaaa gccatcaggg agcaggtcag cccgctgctt gtcggcctcc
     4501 cgcagcctga accggcattt aatatcctgc cggtcaatcc ttcaggcttc cgcgatatct
     4561 atgtctcgca ccggaacatc atacaggtta caattgaccc cggaattcag aaaccggcag
     4621 taacatatac ccgcaacaaa tgggctgaga cacagcttgt tgtaaacatt tcggctgctg
     4681 ataccgcctc gcttgccgca gcagtgaggg agaacagtga tgagatacgc cgcagcatca
     4741 atgatgcgga gagggagagg ctgataacat ggctgacaaa ctccgcgggg aagcatagaa
     4801 gtgtcctgaa tacaggagaa gccagatggg aactggccat tcctgcaggc tggaaaccgg
     4861 acttcaaccg tgatgatatc atgatgttat cgcttgagac cccttcaaca acacagtcgg
     4921 tgatggtatc ggtaaccggc cggacaaccc cgaggatcgg atgcttcgag cttgccgatc
     4981 tgacgcagaa gcgaatgtca gccgaagtga agggtcctga cggcgggtca ttcatggtga
     5041 tagaacagag gatgccggtc agctgccgct cattccggag gtatgatact gattacatag
     5101 agatgagggg attatggacg ctcgagggcg gattcatggg cggcccgttc atatcttatg
     5161 cctttattga ttcggctact gccagggcgg ttattgtcac agggtttgta tatgcgccaa
     5221 aggaggagaa gcgggagttg ctacgccagg ttgaggcact tatgtacacg gtgaaaaggc
     5281 agcagtaata aggatcatgg ccctgccggg cggttgcaat actgtattcc ccttacagcc
     5341 tccggctgcc gggacagact ccggggacaa ttcaccactg ctgacgggtg ccagctttca
     5401 gatttctggc actattcttc caccgggata tacctctgat cacgccttac cggaatgaac
     5461 ccggcctcac ggattatctg ctgcatctca ccggggctga ctcggaacgg gtcaccggcg
     5521 gcgctcacca cattctcctc aatcatcact gatcccaggt cattggcgcc gcagtgaagt
     5581 gagatcatgg ctgtctccct gccaacggtg agtattgatg actggatgtt cggaatattg
     5641 tcaagcatca gccggctgat tgcaatcagt ttcaggtatt ccggtgcggt caccctgctg
     5701 cgtattccat ccttttcagc cagccttgtg ccgcggtcca tgaatggcca gggaataaag
     5761 gttatgaacc cgtcgtgccc ggccggcctg tcatcctgta gtttgcgaag catatcgaga
     5821 tgttcgagcc gctcccgtat cgtttctgcg tggccgaaca tcatggttgc ggaggtcggc
     5881 aggttgagtc tgtgtgcctc cctcatcact cccagccatt ctttgacggt agccttggca
     5941 ggcgaaacga gtttcctgac cctgtcagaa agaatctcgg cgccggcgcc gggaagggaa
     6001 tcaagccctg catcacgaag cgacaccagc acatcatggt agctcatctt cccttgccgg
     6061 gcaagccatg ccacctccgg cggaccgaga gcatgaagct tcaaaccggg ccagcggctc
     6121 ttgagactcc tgaaaagacc cgtataaaaa tcaagcccca gcaccggatt catgcctccc
     6181 tgcagcagaa gctggtcacc tcccagggcc atcagctcca ttattttccg gtcatactcc
     6241 tcatcggtgg tcacataggc atcttcatgc ttttctgtac ggcagaagtt gcagaaccgg
     6301 cactgtgaga agcatatatt ggtgatgttt acattccggt caatgatcca tcctgccctg
     6361 ttgcccggat gaagcctttt tctggtaagg tcggcaacat acatcagctc ctccggcgga
     6421 gcttcattcc acaggttcag ggcctcatca acagttaggc ggccaccacc gtcaagcctg
     6481 tagtatatct gttctctgaa tcccatcctg tggcctcctc agcatattgg cagatgccgg
     6541 taaaggtaat atattaaagc aatatcattc aattttttga ttaactttac ctccctgaac
     6601 agcctctttg ctgttcctca gacctgattt acaatgaata aaacgcctat agttgttggg
     6661 attacccagg gagacatgaa tggcatcggc tacgaggtca taatgaaggc cctctccgat
     6721 tctgagatta ccggtttttg cgtgccggta gtttacggtt cgccaaaagt ggctgcatat
     6781 caccgtaagg tgcttaatat caccaattta aacttcaata acatacgttc tgtcgctgaa
     6841 gcgcacccta agaaggtaaa cattataaac tgccttggcg atgatgtgca tgttgaactt
     6901 gggaagtcga ctcgcgaggg cggtcagtca tcccttgctg ctctggaggc agcggtcact
     6961 gaccttaccg ccggccggat agatgtgatg gtgacagcgc ccattgacaa gagcaatata
     7021 cagtcaggca gtttcaggtt caatggccat actgattggc tcaaatcaaa gaccggatcg
     7081 cacgatgcgc tgatgctcat gatctccgag gccatgaagg tcgggtttgt cacggagcat
     7141 ctgcccctga aggatgtccc gggtgcaatc actatcggga atatccttgc aaagctcagg
     7201 ctgctgaaca gctcgttgct tcaggatttt gcagtacgtg gcccgagaat aggtgtgatg
     7261 ggccttaacc cgcatg
//
LOCUS       DAOPBK010000135         7242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9275_length_7242_cov_4.112982, whole genome shotgun sequence.
ACCESSION   DAOPBK010000135 DAOPBK010000000
VERSION     DAOPBK010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9275_length_7242_cov_4.112982"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLA17_08310"
     CDS             complement(<1..121)
                     /locus_tag="PLA17_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPK85457.1"
                     /translation="MRIVIAGAGEVGTHLAKMLSQSEHEIILIDPAVERLKPVD"
     gene            complement(164..1900)
                     /locus_tag="PLA17_08315"
     CDS             complement(164..1900)
                     /locus_tag="PLA17_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPK85458.1"
                     /translation="MYFRDIPFLRLTMPLCAGVIMAEYLHGIALIVAIVAIIALTLMT
                     VRLLYAGYRSDILFGVCLILFMMTAGYLLRTWEYSRLSDLGSYRQEVTVRLSGYPDPG
                     NSGWSLKARIVSIRSGDTVTAPRGSILLWFMGDTVPSAWKPGDLATVYVRPLPVKNNG
                     NPCEFNYRRYLEGQGVRYMAFFRADDIRAFVPGASRTVRERSLIAAHGMIDLFRRAGL
                     EGETLGLITALTIGDKELLDKERLTSFSRTGAMHIMAVSGLHVGMISMALSWLLFFLR
                     RRLKKVRALIIIPALWGFAFITGLTPSVLRATIMFTFLQAGNILNRPAAGMNNLLASA
                     FIIIAAHPSVIFEAGFQLSYLAVIFIIAFYAPLYRLVKLRNRVADYLWQMTAVSLVAQ
                     AGTMALSVRLFNIFPLMFLLTNIVVIPVSFVVLILAMLLLVSSPVPALASFFAMLLDH
                     LARFTLSFTGMVSSAEHGVITGIGMSAAETIMMTLTLALLLAALLRTARVTLRPFLVA
                     MMLLLTCNTIKTAQEMHRERLITYNIRGERLKVRQHGRVLLVPSSAGGTPAEVQKHAC
                     TRGLKIRVIEPG"
     gene            complement(1966..2526)
                     /gene="def"
                     /locus_tag="PLA17_08320"
     CDS             complement(1966..2526)
                     /gene="def"
                     /locus_tag="PLA17_08320"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPK85459.1"
                     /translation="MTYPIYVYGHPVLRKVAQEIERGYPDLDRFIEDMFETMYESDGL
                     GLAAPQIGKSVRLFVIDGEPLAKDHPEMKEFKKAFINPQVVERKGAIVPVNEGCLSLP
                     GLHEEVDRESEIRIQYYDQQWNYHDEVYTGYKARIVLHEYDHLDGILFPDRLSPLRRR
                     LMKGKLNDISRGKFEATYRTVLPNSR"
     gene            complement(2534..2962)
                     /gene="ruvX"
                     /locus_tag="PLA17_08325"
     CDS             complement(2534..2962)
                     /gene="ruvX"
                     /locus_tag="PLA17_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPK85460.1"
                     /translation="MGRALAIDYGRKRCGVAVTDPARIIASPLLTVPSGELEAFLREY
                     IPQENVTDVIIGYPVTMNNLPSESVKYIDPFIARFRKIFPEIPLHLADERFTSQMALQ
                     TMIEGGVSKSGRRDKGMADRISASIILQSWLTGRENKNRN"
     gene            complement(3094..6654)
                     /gene="nifJ"
                     /locus_tag="PLA17_08330"
     CDS             complement(3094..6654)
                     /gene="nifJ"
                     /locus_tag="PLA17_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPK85461.1"
                     /translation="MAKKKFITCDGNQAASHIAYMFSEVACIYPITPSSTMAVYIDEW
                     AAHGLKNMFGEEVKVVEMQSEAGAAGAVHGSLQSGALTSTFTASQGLLLMIPNMYKMS
                     GELLPAVFHVSARTVAAHALSIFGDHSDIYATRQTGFAMLATGSIQEIMDLAGVAHLA
                     AIRARVPFLHFFDGFRSSHEIQKIEEISIEDMKKLVDKDALAAFRQRALNPCKPVIRG
                     TAQNPDIFFQAKEASNRFYTNLDDTVNEYMQKITQITGRTYKPFTYYGAPDAENIIIA
                     MGSVTETIKEVIDYLAETKGEKLGLISVHLYRPFSAKYLFNVLPESVRRITVLDRTKE
                     PGANGDPLYLDVKDVFYGKKNQPEIIGGRYGLSSKDTTPAQMISVFENMKKEKPMNQF
                     TVGIVDDVTFRSLPQLPEINVSHKGTYSAKFYGIGSDGTVGANKNSIKIIGDTTDKYC
                     QAYFAYDSKKSGGVTTSHLRFGDHPIRSPYLVNTPDFVACHVPVYLDKYDMLKGLKKG
                     GTFLLNSIWDVEETKSKLPDHMKKYLAENNINFYIINATRIAEELGLGTRTNTIMQSA
                     FFKVSEVIPYKLAVDEMKNAVITAYGRKGENVVKMNNDAIDRGGDVTRVEIPAEWAGI
                     KVAEVKEETGKPAFIRDVVDVINGQRGDDLPVSAFVGREDGTFPSGTAAYEKRGIAVN
                     VPEWQVDNCIQCNQCSYVCPHAAIRPFLLTDEELAAAPEGTVAKQGIGNTKGYKFKIQ
                     VSVYDCTGCGNCADVCPSKEKALIMKPLETQLAEADRWTYMHEKVGYKDTVVDKYANV
                     KNSQFAQPLFEFSGACAGCGETPYIKAITQLFGDRMVIANATGCSSIYGGSVPSMPYT
                     VNKNGHGPAWANSLFEDNAEYGLGIRLGATKIRDRIERLMKESLGNGLAPETVAAFNE
                     WIAEKDNTKATRAVSEKVIAACEKDKAPVCREILGLKSYLVKKSFWVFGGDGWAYDIG
                     YGGLDHVLASGENINVLVLDTEVYSNTGGQASKSTPAGAVAKFAASGKKIRKKDLGHM
                     AMTYGYVYVAQVAMGSSQSQYLKAIREAEEYPGPSLIIAYSPCINHGLRKGMGKTQQQ
                     ARDAVEAGYWHLYRFDPRLEAEGKNPFQLDSKEPDWSKFQEFLKSEVRYTSLIKDFPG
                     EAKVLFRQAEENAKWRYNNYKRLSLLEYGNTSAETETKTE"
     gene            complement(6732..>7242)
                     /locus_tag="PLA17_08335"
     CDS             complement(6732..>7242)
                     /locus_tag="PLA17_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPK85462.1"
                     /translation="KRLVECGHDVVILLDSITRLARAFNTIAPASGKVLSGGVDSNAL
                     HKPKRFFGAARNIENGGSLTIIATALTDTGSKMDDVIFEEFKGTGNMELQLDRKLSNR
                     RIFPSIDIPASSTRREDLLLDKNMLNKIWILRNYLTDMNSVEAMEFLRDRISQTRSNE
                     EFLISMNSE"
BASE COUNT         1621 a         1880 c         1882 g         1859 t
ORIGIN      
        1 cgtcaacggg tttgagacgc tccacggccg gatcaatgag tatgatctca tgttcactct
       61 gcgagagcat cttggcaagg tgtgttccca cctctcctgc accggcgata actattctca
      121 tatctggaac aataaaacag ggcaaagtta tacaaaagaa atgttacccc ggttcaatga
      181 ccctgatctt tagtccccgg gtacaggcat gcttctgcac ctcagccgga gtgccgccgg
      241 cagatgacgg caccagcagc actctgccgt gttgccgtac ctttagcctc tctcccctta
      301 tattgtaggt gatcagcctt tcgcgatgca tctcctgtgc tgtcttgatg gtgttgcatg
      361 tcagcagaag catcatggca accaggaacg gcctgagggt tacccgggct gtgcgcagga
      421 gtgcggcaag gagaagggct aacgtcaggg tcatcatgat ggtctcggca gctgacatgc
      481 caatacctgt tatcacacca tgttcagccg agcttaccat tccggtgaag ctgagggtga
      541 aacgagccag atggtcaagc agcatggcaa agaatgatgc cagcgccgga acgggagagg
      601 atacaagcag gagcattgcc agtatcagca ccacgaacga gaccgggatt accactatgt
      661 tggtaagaag aaacatcagg gggaagatgt tgaagagcct gactgacagt gccattgtcc
      721 ctgcctgggc taccagtgac accgctgtca tctgccacag gtaatcggca acacgattcc
      781 tgagtttcac cagcctgtaa agcggagcgt agaatgcaat gatgaagatc acggcgaggt
      841 aggagagctg gaatcccgcc tcgaaaatga ctgaagggtg ggcggcgatg attataaagg
      901 ccgaagccag caggttgttc atccccgcgg ccggccggtt gagaatattg cctgcctgaa
      961 ggaaggtaaa cataatggtt gccctgagaa ctgagggggt gaggcccgtt atgaacgcaa
     1021 agccccacag cgcggggatg atgatcagcg ccctcacctt cttcagccgt cggcgcagga
     1081 agaagaggag ccaggagagg gccatgctta tcatgcccac atggagacct gacactgcca
     1141 ttatatgcat tgcacctgtc cttgagaagc ttgtcagccg ttccttgtca aggagctcct
     1201 tgtcgcctat tgtaagggca gtgataagac caagtgtctc accctccagg ccggcccggc
     1261 gaaaaagatc aatcatgcca tgtgcagcga tgagcgatct ttcccggact gtccggctgg
     1321 cgccgggaac gaaagccctg atgtcatctg ccctgaagaa cgccatatac ctgaccccct
     1381 gtccttcgag gtaacgccga tagttgaatt cgcatggatt gccgttgttc ttcaccggca
     1441 acggcctgac atacactgtt gccaggtcac cgggcttcca ggcggaagga acggtgtcac
     1501 ccatgaacca tagaagaatt gagccccgtg gcgcggttac cgtatcacct gacctgatgc
     1561 tgacaatgcg tgctttcagg gaccagccgc tgttgcccgg gtcaggatag cccgacagac
     1621 gtactgtcac ctcctgccgg taactgccaa ggtcgctcag cctgctgtat tcccaggtgc
     1681 gtagcaggta gccggcggtc atcataaaaa ggatcaggca tacgccgaag aggatgtcag
     1741 aacggtaccc ggcatacaac agccttacag tcatcagggt gagcgcaata atagctacga
     1801 ttgccacaat cagggcaata ccatgcaggt attctgccat gattactccg gcacacagcg
     1861 gcatggttag cctcaggaac ggtatatccc tgaaatacac gggtaagcag cattaagacc
     1921 catgtaagtt aagaaatatg tcaatacgga tcaatggcag gttatttacc tgctgtttgg
     1981 gagcacagtg cggtacgtgg cttcaaattt tcccctgctg atgtcattca gtttgccttt
     2041 catcaggcgt cgccgaagag gactcagcct gtcaggaaag agaattccgt caagatgatc
     2101 atactcatga agcacgatcc tggccttgta gccggtgtag acctcgtcat gatagttcca
     2161 ctgctggtca tagtactgta tacgtatctc tgactcacgg tctacctcct catgaagacc
     2221 cggcaggctc aggcacccct cgttcacagg cactatggcg cctttgcgtt caaccacctg
     2281 cgggttgatg aaggcctttt tgaattcctt catctcaggg tgatctttgg caagaggttc
     2341 accgtcaatg acgaaaagcc tgactgactt accaatctgt ggggcagcaa ggccaagacc
     2401 gtcagactcg tacatggttt caaacatatc ttcaatgaac ctgtcaaggt cagggtaacc
     2461 tctctctatt tcctgtgcca cctttctcag gacgggatgt ccgtatacat agatagggta
     2521 agtcatctct gtttcagttc ctgtttttgt tttcccggcc tgtcagccat gactggagga
     2581 taatggaagc gctgatacgg tcagccatgc ccttgtcacg ccttccgctt ttgcttactc
     2641 ctccttcaat cattgtctgc aatgccatct gtgaggtgaa tcgttcatcg gccaggtgca
     2701 ggggtatttc cggaaagatt ttcctgaacc gggcaatgaa cggatcaatg tatttaacgc
     2761 tttcgcttgg cagattgttc atggtcaccg ggtaaccgat aatgacgtcc gttacgtttt
     2821 cctgcgggat atattcgcgc agaaaagcct ccagctctcc tgacggaacg gtcagcaggg
     2881 gagaggctat gatccttgcc ggatcagtga ctgccactcc gcatctcttc cttccgtagt
     2941 ctattgccag cgctctgccc attttgccca ttctgttccg gctttatgcc gctctgttca
     3001 gccggtatta tgtcgggtgc aaagataccg gttttccttt ataaacagga gagagccggc
     3061 actggccggc tctctcttcg ttatataaca gctttattct gttttggttt cagtttctgc
     3121 ggatgtgttg ccatactcca gcagagagag gcgcttgtag ttgttgtatc tccattttgc
     3181 gttctcctct gcctgcctga acagcacctt tgcctcgccg gggaagtcct tgataagcga
     3241 tgtgtagcgc acctctgact tgaggaactc ctggaatttg ctccagtcgg gctcctttga
     3301 gtcgagctgg aacgggttct tgccctcagc ttcaaggcgc ggatcgaacc tgtaaaggtg
     3361 ccagtatcct gcttcaactg cgtccctggc ctgctgctgt gtcttgccca tgcctttgcg
     3421 gaggccgtgg ttgatacagg gtgagtaggc gattatgagt gacggaccgg gatactcctc
     3481 agcttcacgt atagccttga ggtactggct ctgactcgag cccattgcaa cctgtgccac
     3541 atatacatat ccataggtca tggccatatg cccgaggtcc ttcttgcgaa tcttcttgcc
     3601 ggaagcggca aacttggcaa cagcgcctgc aggtgtcgac ttcgaagcct ggcctccggt
     3661 attggagtag acttcagtgt caagcacgag aacgttgatg ttttcgcctg aggcgagtac
     3721 gtggtcaagg ccgccatagc ctatgtcata agcccatccg tcaccgccga acacccagaa
     3781 cgacttcttg accaggtagc tcttcagtcc caggatctcc ctgcaaacag gagccttatc
     3841 cttttcgcaa gctgcgataa ccttttccga aacagccctg gtggcctttg tgttatcctt
     3901 ttcggcaatc cactcattga atgctgccac ggtctccggt gcaagtccgt taccgaggct
     3961 ctccttcatc aggcgctcaa tccggtctct gatcttcgtt gctcccagcc tgatgccaag
     4021 gccatactca gcgttgtctt cgaacagtga attggcccat gccggaccgt gaccgttctt
     4081 attcacggta taaggcattg aaggaactga accgccataa attgatgaac aacctgtggc
     4141 gttggcgatg accatgcggt caccgaaaag ctgggtgata gccttgatat aaggtgtctc
     4201 accgcatccg gcacatgctc cggagaactc gaagagcggc tgtgcaaact gagagttctt
     4261 gacattggcg tacttgtcaa ccacggtatc tttataaccg accttttcat gcatgtaggt
     4321 ccacctgtca gcttctgcaa gctgtgtttc cagaggcttc atgataagtg ccttttcctt
     4381 tgaggggcat acatcggcac agttgccgca gccggtacag tcatagacac ttacctgtat
     4441 tttgaactta tagcccttgg tgttgccaat accctgtttt gcgaccgtac cttcaggcgc
     4501 tgctgcaagc tcctcgtcag tcaggaggaa gggacgtatt gcagcgtgcg gacagacata
     4561 ggagcactgg ttgcactgta tacagttgtc tacctgccac tcaggcacgt tgacagcaat
     4621 tccgcgcttt tcatatgctg ctgttcctga ggggaatgtg ccatcttctc gtccgacaaa
     4681 cgcgctgacc gggagatcat cacccctctg gccgttgatg acatcaacaa catcacgtat
     4741 gaatgcaggc ttgccggtct cttccttcac ttcagccacc tttatgccgg cccactctgc
     4801 aggtatttca accctggtaa cgtcaccacc gcgatcgatg gcgtcattgt tcatcttcac
     4861 aacgttctcg ccctttctgc cataagcagt aatgacggca ttcttcatct cgtcaacagc
     4921 aagtttgtag ggaattactt ccgatacctt gaagaaggcg ctctgcatga tggtgtttgt
     4981 ccttgtgccc aggcccagtt cttcagcaat tctggttgca ttgatgatgt agaaattgat
     5041 attgttttct gcgagatact tcttcatgtg atcaggcagc ttgctctttg tctcttcaac
     5101 atcccagatg gagttcagga ggaaggtgcc tcccttcttg agacccttga gcatgtcata
     5161 cttgtcaagg taaaccggta cgtggcacgc cacgaaatcg ggagtgttga caagataggg
     5221 cgaacggatg ggatgatcac cgaaacggag atgtgatgtt gtcacaccgc ccgatttctt
     5281 tgagtcatat gcgaagtaag cctggcaata cttatcggtt gtatcaccta tgatcttgat
     5341 agagttcttg ttggcgccca ctgtaccgtc agagccaata ccgtagaact tggccgaata
     5401 ggtacccttg tggctcacgt tgatctcggg aagctgcggc agcgacctga aggttacgtc
     5461 gtcaacgatt cccacagtga actggttcat gggcttctcc ttcttcatgt tctcaaaaac
     5521 cgagatcatc tgtgcaggag tggtatcctt cgagctgaga ccgtagcgtc cgccgatgat
     5581 ttccggctga ttcttcttgc cgtagaatac atccttgacg tcgagataga gcggatcacc
     5641 gttggctccg ggctccttcg tacggtcaag aacagtaatg cgcctgacac tctccggcag
     5701 gacattgaag aggtattttg ctgagaaggg cctgtagagg tggacactga taagcccgag
     5761 tttctcgccc tttgtttctg caagataatc aatgacttcc ttgatagtct ccgtgacaga
     5821 ccccatggca atgataatgt tttctgcatc aggtgcaccg taataggtaa acggcttgta
     5881 ggtacgcccg gtaatctggg tgatcttctg catgtactca ttgactgtat cgtcaaggtt
     5941 ggtgtagaag cggtttgatg cctccttagc ctggaagaag atgtcagggt tctgagctgt
     6001 tcccctgata accggtttgc agggattgag ggctctctga cggaatgctg cgagagcatc
     6061 cttgtcaaca agctttttca tatcctcaat gcttatctcc tcaatcttct ggatctcgtg
     6121 tgatgaacgg aatccgtcga agaaatggag gaagggaact cttgccctga tggctgcaag
     6181 gtgtgcgacg cctgccaggt ccattatctc ctgaatgctg ccggtagcca gcattgcgaa
     6241 acctgtctgg cgggtggcat agatgtcact atggtcaccg aagattgaga gtgcatgtgc
     6301 agcaaccgta cgtgcgctga catgaaacac tgccggaagg agttcgcctg acattttata
     6361 catgttggga atcatgagga gaagcccctg tgatgcggtg aaggtagatg tcagtgctcc
     6421 tgactgaagc gaaccgtgta ctgctcctgc ggcaccggct tcgctctgca tctcaacaac
     6481 ctttacctct tcaccgaaca tgttcttcag tccgtgtgct gcccactcat ctatgtatac
     6541 tgccatggtg gaggagggag taataggata gatgcaggct acttcgctga acatataggc
     6601 tatgtgcgat gcggcctggt tgccgtcaca agtgataaat tttttctttg ccatttctga
     6661 ttataagtaa taattgatca attaaccttt cgaaaaataa agtgcaaatg tactcatttt
     6721 ttacgagaat cttactcgct gttcattgaa atgaggaact cttcgtttga gcgtgtctgc
     6781 gagatgcggt cacgcaggaa ctccatggct tccactgagt tcatgtcagt gaggtagttg
     6841 cggagtatcc atattttgtt aagcatgttc ttgtccagaa gaaggtcctc acgtctggta
     6901 cttgaagccg gtatgtcaat tgacgggaat atcctcctgt tggagagctt gcggtcaagc
     6961 tgcagctcca tgttgcctgt tcccttgaac tcttcaaaga tgacatcgtc catcttcgac
     7021 cctgtgtcag tgagtgccgt ggcaatgatg gtcagtgagc cgccgttttc aatgttgcgg
     7081 gctgcgccga agaagcgttt cggtttgtgg agggcgttgg agtcgacacc gcctgagagc
     7141 accttgcccg atgccggggc gatggtgttg aatgcccttg ccagacgggt gatggagtca
     7201 agcagaatga ccacgtcatg accacactcc accagcctct tg
//
LOCUS       DAOPBK010000136         7229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9289_length_7229_cov_4.621829, whole genome shotgun sequence.
ACCESSION   DAOPBK010000136 DAOPBK010000000
VERSION     DAOPBK010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9289_length_7229_cov_4.621829"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLA17_08340"
     CDS             complement(<1..782)
                     /locus_tag="PLA17_08340"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPK85463.1"
                     /translation="MQPESNATAVMARQFFIEVNSQQRNHHGERICGDVFLSENVREE
                     NRIITVLSDGMGHGVKANILATLTSTMALNFTREHKEPDRIAEIIMNTLPVCSERKIS
                     YATFSIVDVESDGRTNILEYDNPRCIILRGSQPFEPDWKDVVLDKGRNAGKVLHKCTF
                     YPAKEDRIILLSDGVAQSGMGSAAWPLGWERDNVRQYASSLIAGDSSISASTLAAKVV
                     NMAYKHDNYEAKDDISCAILYFREPRKLLICTGPPYDEEKDK"
     gene            complement(766..2637)
                     /locus_tag="PLA17_08345"
     CDS             complement(766..2637)
                     /locus_tag="PLA17_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPK85464.1"
                     /translation="MPDQVIKLNEDKCRNSYSCVRVCPVNAIEVRPERAHPVIMADRC
                     IGCGLCFLACSPGAIEYRDSRSQVKQILKSGRPVAALLAPSIASEFDDITDYRKFAGM
                     IRSLGFSYVHEVSFGVDLVAYAYRRLFEESSGKYYITSNCPSIVEMIEKYHPNLVPNL
                     APLVSPMIATAMVTRSLYGDEVVNVFIGPCIDIKAEAARYSDRNLVEAVLTFIEIRQL
                     FDEHEIQEKTVKMSDFDPPCGNWGALYPYPAGILQAAGIKRDLVSSHVITASGAEDVK
                     EAINDFDHHIDTIKHHFNLFFCQGCLLGPGMIRHDERFRRRSLVKQYAEKRVSTLDKK
                     QWKEDMERWSDLDLSRRFSVNDQRIPDPSPEAITRVLKIIGRDNRAEELNCNACGYGS
                     CREFAAIVAKGLAIPEMCHTYNLRNKQEYIETLRQTNKKLSETKKALKESEEKAMREK
                     DAAEDASETMNSMLNKLPNGVVIVDNELKILQSNKRFLEIIGEDAEDIAEIIPGLRGA
                     DLKTLLPFSVYNIFSFVLRENESVISRDVQLEDRMFNISIFPIRQNRIAGAVIRDLFS
                     PEVQREEVINRVSDVIDKNLEMVQKIGFLLGEGASDTEKMLNSIVESFRQKKDATGK"
     gene            complement(2735..3058)
                     /locus_tag="PLA17_08350"
     CDS             complement(2735..3058)
                     /locus_tag="PLA17_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPK85465.1"
                     /translation="MEKIHCLAGALENVVFLSKYELNRRVLGQSNNIEICLGSSCFSR
                     GNKEIVQIIKDFLKKNHLTDKVLLRGARCMNRCSEGPFIIINGMTFSSIGVQDIEKIL
                     ENELV"
     gene            3140..3478
                     /locus_tag="PLA17_08355"
     CDS             3140..3478
                     /locus_tag="PLA17_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85466.1"
                     /translation="MFKASKIDDVLSRYGAGSHEDLIPVIKYIRGWWTDRHSTAADLI
                     PVIQAVQEEGYFTGSAIVRTGSFLRMFTTRIHGLAISHDSFRFIPAGRGSAFTAIIPG
                     SQVSKSITCI"
     gene            3475..4131
                     /locus_tag="PLA17_08360"
     CDS             3475..4131
                     /locus_tag="PLA17_08360"
                     /inference="COORDINATES: protein motif:HMM:NF003996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPK85467.1"
                     /translation="MKALPGKTVERLSQYRRALASVLAANKDYIFSHELAAMLHITAV
                     QVRRDLMLIGYGSVMRKGYDVRELINTIGSIIDAPDGLNVAIVGMGNLGRAIAGYFRD
                     RRTNMTVVAAFDVDPRKTDRVVAGVRCYDISQLRRLREELDIVVAVLAVPADQAVATA
                     SRLVSDGIRGIMNFTTVPLNVPDTVYLEEFDMITYIEKVAYFVKENQPPDFRPGKNAC
                     "
     gene            4247..5668
                     /gene="hydG"
                     /locus_tag="PLA17_08365"
     CDS             4247..5668
                     /gene="hydG"
                     /locus_tag="PLA17_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPK85468.1"
                     /translation="MKFNPEKCTIPDRRMEPFIDPAELWAHINNNKADAVRVREVIAK
                     SLSKERLTLAETAVLINAEGDLVEEIKEGARELKKKVYGNRIVLFAPLYIGNKCTNNC
                     QYCGFRTSNREAKRKTLTDNEIIAEVEALEDNGQKRLILVYGEHPEYGAEYIAHTVRL
                     VYGVKKGRGEIRRVNINAAPLDIDGFRTVGRAGIGTYQIFQETYDPEAYKWYHLGGKK
                     KDYEWRLTALDRAQEAGIDDVGIGALFGLYDWRFEVMGLVRHTNHLEACYNVGPHTIS
                     FPRIKDASSLNITDKYFTSDDDFKKLIAILRLAVPYTGLILTAREAPEVRSEAMAFGV
                     SQIDGGTRLELGSYSDSKNEEQNLNREQFRINDDRSLASIINELIDNDYIPSFCTACY
                     RLGRTGEHFMEFSVPGFIKRYCTPNAILTLSEYIVDYASPGLAEKGWQAIEKNIATLD
                     EGMKQSIRKKIERIRNGERDLYY"
     gene            5680..6204
                     /locus_tag="PLA17_08370"
     CDS             5680..6204
                     /locus_tag="PLA17_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85469.1"
                     /translation="MVRVIGIKITDRIKEAGLVQKVLSEHSSVISTRLGFHELNDELC
                     SREGFMVIHLAGNVDDCGNLVAKLKGIEGLFVREMVFSHGLSDAPLECEGTTIIGIMV
                     PGRNDLGPTVQALLTTYGCVIRTRLGVNEVYYGEPAGLIILELAGNRNQFLSLERSLR
                     EIEELAIGRICFPA"
     gene            complement(6235..>7229)
                     /locus_tag="PLA17_08375"
     CDS             complement(6235..>7229)
                     /locus_tag="PLA17_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85470.1"
                     /translation="WSDTYAPGALYMLQSAGFTARVATAPFTALLSDGSRKQFGYGTI
                     MVHKGDKPVSDGSLMKFLSAVAKECGITMYGVSTGLTPAGMDLGSGGFAVLSRPSVML
                     VIDDGTPADDAGEIWHLLDTRYSMPVTLISPSRMGTANLGRYNVIIIAGNPSISSLAV
                     DRLREWNRSGGTLIGYRGGNRWLADNGFAQITYAESAATPPGLSARYADRGMERAIHT
                     VPGTIFNAVIDSTHPLCYGYTGTHLPVFKTDASAVKITGSSYNTPVKYTADPLLSGYC
                     SKENQERIAGSAVVSVHPGPGRVISIYDDTNFRAIWYGTSKLFVNAVFFGQILR"
BASE COUNT         1755 a         1811 c         1871 g         1792 t
ORIGIN      
        1 tccttatcct tctcctcatc atagggagga ccggtacata tcagcagctt ccggggttcc
       61 ctgaagtaaa gtattgcaca tgagatgtca tcctttgcct cgtaattgtc atgcttgtac
      121 gccatgttga caaccttggc tgccagtgtg gaggcagaaa ttgacgaatc accggcaata
      181 agcgaggagg catactgcct gacgttgtcg cgctcccagc ccagtggcca ggctgctgat
      241 cccataccgc tttgcgcaac gccatcggag agcagtatta tacggtcctc cttggccggg
      301 taaaaggtgc atttgtgcag taccttgccg gcatttctcc ctttgtcaag caccacatcc
      361 ttccagtcgg gctcaaatgg ctggctgcct ctgagtatta tgcatcgcgg gttgtcatac
      421 tcgagtatgt tggtacgccc gtcactctca acgtcaacta ttgaaaaggt agcatagctg
      481 atcttccttt cggaacatac cgggagcgta ttcatgataa tctccgcgat acggtcgggc
      541 tctttgtgct cgcgggtgaa gttgagtgcc atggtggagg tgagggttgc caggatgttg
      601 gcctttaccc catggcccat gccgtcagag agaaccgtta ttatccggtt ctcctcacgc
      661 acgttctctg agaggaaaac atcaccgcag atgcgctcac catgatgatt gcgctgctgg
      721 ctgttgactt caatgaagaa ctgcctcgcc attactgccg tggcattact ttccggttgc
      781 atcttttttc tggcggaatg attcgacaat ggagttgagc atcttttcgg tatctgaggc
      841 tccttcaccc agcaggaacc ctatcttctg aaccatttca aggttcttgt caatgacatc
      901 ggaaaccctg ttgatcacct cctcccgctg tacctcggga gagaagaggt cgcggatgac
      961 cgccccggca atcctgttct gcctgatggg gaagatggag atgttgaaca tccggtcttc
     1021 aagctgcacg tcacggctta tcaccgattc gttttcacgc agcacaaagg agaagatatt
     1081 gtaaacgctg aatggcagca gtgtcttcag gtcggctccg cgaaggcccg ggatgatttc
     1141 tgcaatatcc tccgcatcct cacctatgat ctccaggaaa cgtttgttcg actggagtat
     1201 cttcagctca ttgtctacaa tcacaactcc gtttggcagc ttgttaagca tgctgttcat
     1261 ggtttcagag gcatcctctg cagcatcctt ttcgcgcatg gccttttcct ccgactcttt
     1321 cagggctttt ttcgtctccg agagtttctt gttggtctgc ctgagtgttt caatatactc
     1381 ctgtttattg cgcaggttgt aggtgtggca catctcagga atggcaagcc cctttgccac
     1441 aatggctgca aactccctgc atgatccgta accgcatgcg ttgcagttga gctcttcggc
     1501 acgattgtcc ctgccgatga tcttcagcac cctggtgatt gcttcaggag acgggtccgg
     1561 aatccgctgg tcattgacgc tgaaccggcg cgagagatcg agatctgacc accgctccat
     1621 atcctctttc cattgttttt tatcaagggt actaacccgc ttctcggcat actgcttcac
     1681 aagtgaccgg cgcctgaacc gttcgtcatg cctgatcatg cccggaccaa gcaggcatcc
     1741 ctggcagaag aagaggttga aatggtgttt gatggtgtca atatgatggt cgaagtcatt
     1801 gatcgcctcc ttgacatctt cagcccctga agcggtgatc acatgacttg ataccaggtc
     1861 acgctttatg cctgcagcct gaaggatgcc cgcagggtag gggtagagag caccccagtt
     1921 gccacaggga ggatcaaaat cagacatctt gactgtcttt tcctgtatct catgctcatc
     1981 aaagagctga cgtatctcta tgaatgtaag gacggcttcc acaaggttcc tgtcactgta
     2041 gcgggcagct tctgccttga tgtcaatgca gggcccgatg aaaacgttga caacctcgtc
     2101 accataaagg ctgcgggtaa ccattgccgt ggcaatcatg ggcgagacca gaggtgcaag
     2161 gttgggaacc aggttcggat gatacttctc tatcatctcc actatggagg gacagtttga
     2221 agtgatataa tacttgccgc ttgactcctc gaaaagcctc ctgtaagcat aggcaaccag
     2281 gtcaacgcca aaggatacct catgaacata ggagaacccc aggctccgta tcatcccggc
     2341 aaacttgcgg tagtctgtta tgtcatcaaa ctccgaagct atgctgggag ccagcagggc
     2401 tgccacagga cgccctgact tcagtatctg ctttacctgg cttcgcgaat ccctgtactc
     2461 aattgccccg ggagagcatg ccaggaaaca gagtccgcac cctatgcacc tgtcagccat
     2521 aatcaccgga tgagccctct caggccttac ctcgatggca ttcacagggc acaccctcac
     2581 acatgaataa gagttgcggc acttatcctc gtttaatttt attacctgat caggcatctt
     2641 cataagcagt taatcggttt ccaggtacat attaaacctt cacatgattg agacctggaa
     2701 tcacattggt tggttatcgg ttttcaagta tattctaaac cagctcattt tccagtatct
     2761 tttctatatc ctgtacccca atgctggaaa aagtcatccc gttgatgatt ataaacggac
     2821 cctcactgca acggttcatg cagcgcgctc ctcttagcag caccttgtct gtaaggtgat
     2881 tcttcttcag aaaatctttg atgatctgaa caatctcctt gttacctctt gaaaaacaag
     2941 aacttccaag acagatttca atgttgtttg attgtcccaa taccctcctg tttagttcat
     3001 acttactcaa aaatacaaca ttttccaacg ctcctgccag acaatggatc ttttccataa
     3061 ttatttgtta atagattaac aatgtttttg aaataacaat aaaaacaaat aattttgtat
     3121 ttgtctgaat agtcagattg tgttcaaagc gagcaagata gatgatgttc tgagccgtta
     3181 cggggcggga agtcatgagg atcttatacc cgttattaag tatatccggg gctggtggac
     3241 agatcggcat agtacggcgg cagatcttat acccgtcatt caggccgtac aggaggaggg
     3301 ttacttcacc gggagcgcta ttgtacgtac cggcagtttc ctcaggatgt tcacaaccag
     3361 gatacacggt ctggccatct cccatgatag tttcaggttc attccggctg gcaggggatc
     3421 agccttcacg gcaataattc ccggatcaca ggtttcaaaa tcaataacat gtatatgaaa
     3481 gcacttccag gaaaaaccgt tgaacgttta agtcagtaca ggagagcgct tgcctccgta
     3541 ctggctgcaa acaaagatta tattttttcg catgagcttg ccgcgatgct ccacatcacc
     3601 gctgtgcagg tcaggcgcga tctcatgctt atcggatacg gcagcgtgat gcggaagggt
     3661 tatgatgtga gggagcttat caatactatc ggatcaataa tcgatgcccc cgacggcctc
     3721 aacgtggcca tcgtcgggat gggcaacctg ggaagggcca tagccggata tttcagggac
     3781 aggcggacta acatgaccgt ggtggccgcc tttgatgttg acccccggaa gacagacagg
     3841 gtggtggccg gggtgcgttg ctacgatata agtcagctca ggaggctgag ggaggagctt
     3901 gatatcgtgg ttgccgtgct ggccgttccg gctgatcagg ccgtagccac agcctcacgg
     3961 ctggtcagtg acggaatcag gggtatcatg aacttcacaa cagtgcccct caatgtacct
     4021 gacaccgtct atctcgagga gtttgacatg ataacataca ttgagaaagt ggcctacttt
     4081 gtcaaggaaa accagccgcc tgacttcaga cccggcaaga acgcttgttg aaggtactga
     4141 agagatgaag gagttaattt tctgaccgga ttgaacaatt ggcccccttt ttacgtatct
     4201 ttacaagtta atttctgatc gggggtgtaa ttaaaattta agagaaatga aattcaatcc
     4261 tgaaaaatgc accattcctg accgcaggat ggagccgttc attgatccgg cagagctgtg
     4321 ggcacatata aataataaca aggctgatgc cgtaagggtc agggaggtga tagcaaagtc
     4381 gctttccaag gagcgtctca ccctggctga gacagctgta cttatcaacg ccgaaggcga
     4441 cctggtggag gagataaagg agggtgcgcg cgagctgaag aaaaaagtct atggcaacag
     4501 gatagtgttg tttgcacccc tttacatagg aaacaagtgc accaacaact gccagtactg
     4561 tggtttcaga acctccaaca gggaggcaaa gcgcaagacc cttaccgata acgagatcat
     4621 tgcagaggtt gaagcactgg aggataacgg tcagaagagg ctgattctcg tttacggtga
     4681 gcacccggag tatggcgcgg agtatattgc acatacggta cggctggttt acggagtcaa
     4741 gaaaggccgt ggtgagatac gcagggtgaa catcaacgct gcaccgcttg acattgacgg
     4801 gttccgtacc gtgggccggg cagggatagg tacataccag atattccagg agacctatga
     4861 ccctgaggca tacaaatggt accacctcgg cggaaagaaa aaggattatg aatggcgtct
     4921 gacagctctc gacagagccc aggaggcagg aattgacgat gtgggcatag gcgccctgtt
     4981 cggcctctat gactggcggt tcgaggtgat gggcctggta aggcatacca atcacctgga
     5041 agcctgctat aatgtaggcc cccataccat ctccttcccg cgtataaagg atgcctcgtc
     5101 actgaacatc accgataagt acttcaccag tgatgacgac ttcaagaagc ttatagccat
     5161 tctccggctt gcagtgcctt ataccggact gatactaact gccagggagg ctcctgaagt
     5221 ccgttccgaa gctatggctt tcggggtgtc acagatagac ggaggaacca ggcttgaact
     5281 gggcagttac tcagactcaa agaatgagga gcagaacctt aaccgcgagc agttcaggat
     5341 caatgatgac agatcactgg caagtattat caatgagctg atagacaatg actacatccc
     5401 gtcgttctgc acggcatgct atcgcctggg ccgtaccggc gagcacttta tggagttctc
     5461 ggtgcccggc tttatcaaga ggtattgtac acccaatgcc attcttacgc tctcagaata
     5521 cattgttgac tacgcttcgc ccgggctggc agagaaggga tggcaagcca tcgaaaagaa
     5581 cattgccacc cttgatgagg ggatgaagca gagcataagg aaaaagattg agcgtatcag
     5641 gaacggggag agagaccttt actactaaaa tacactatca tggtcagagt aataggcata
     5701 aagatcactg acaggatcaa ggaggcagga cttgttcaga aggtactctc tgagcactct
     5761 tctgttataa gcaccaggct cgggtttcac gaactgaacg atgagctgtg cagccgcgaa
     5821 ggatttatgg taatacacct tgcaggaaat gttgatgact gcgggaatct ggttgcgaag
     5881 ctgaagggaa tcgaggggct gtttgtcagg gagatggtat tctctcacgg gctgtcagat
     5941 gctcccctgg agtgtgaggg caccaccatt atcgggatta tggttcccgg gagaaatgat
     6001 cttggcccca cggtacaggc gctgctgacc acctacggat gtgttatccg caccaggctg
     6061 ggtgtcaatg aggtttacta cggggaaccg gccggactga tcatccttga acttgcaggc
     6121 aacaggaacc agtttctttc ccttgagcgc tcgcttcgcg aaatagagga gctggcaata
     6181 ggcaggatat gttttcctgc ctgagagagc agactccttt acagagcggt gtccctatct
     6241 gagtatctgt ccgaaaaaga cagcgttgac aaaaagtttc gaggtgccat accagatggc
     6301 cctgaagttg gtgtcgtcat atatcgaaat aacccttccc ggtcccggat gaaccgacac
     6361 aaccgcactg cctgcaatgc gctcctggtt ttcttttgag cagtatccgc tcagcagcgg
     6421 gtcagctgta tatttcaccg gtgtgttata actgctgcct gtgatcttca ccgctgatgc
     6481 atcggttttg aataccggca gatgggtgcc ggtgtatccg taacagagcg ggtgagtaga
     6541 gtcaatgaca gcattgaaga tcgttccagg aactgtatgt atcgccctct ccattcccct
     6601 gtcggcatac cttgcagaga gaccaggtgg tgtggcagca ctttctgcgt aggttatctg
     6661 ggcaaagccg ttgtcggcca gccatctgtt cccgcccctg taaccaatca gggttccgcc
     6721 ggaacggttc cattcgcgca ggcggtccac tgccaaagat gagattgacg gattccctgc
     6781 tatgatgatg acgttgtacc tgccaaggtt ggccgtaccc attcttgacg gggagatgag
     6841 tgtcacaggc atgctgtagc gtgtgtcaag aaggtgccag atttcaccgg catcgtcggc
     6901 aggtgtccca tcatcgatta ccagcatcac cgaaggtctt gacagcactg cgaagccgcc
     6961 gctgccaagg tccatgccag cgggggtgag tccggtggag acgccgtaca tggttatgcc
     7021 gcactccttc gccactgcgc tgaggaactt catgagcgaa ccgtcagaaa ccggcttatc
     7081 tcccttatga accattatgg tgccgtaacc aaactgcttc ctgctgccgt cactcagtaa
     7141 agccgtgaaa ggcgctgtgg ccacccgtgc ggtaaagcct gcgctctgta acatgtaaag
     7201 tgccccgggg gcataggtgt cactccatt
//
LOCUS       DAOPBK010000137         7188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9338_length_7188_cov_4.349783, whole genome shotgun sequence.
ACCESSION   DAOPBK010000137 DAOPBK010000000
VERSION     DAOPBK010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9338_length_7188_cov_4.349783"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLA17_08380"
     CDS             complement(<1..214)
                     /locus_tag="PLA17_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HPK85471.1"
                     /translation="MKKILIMLFIVSLGVISCKNESCENPFYCDWDTPFEVPPFDKIK
                     FEHFKPAFLKGMEEEMAEVEAIINNPE"
     gene            complement(323..2065)
                     /locus_tag="PLA17_08385"
     CDS             complement(323..2065)
                     /locus_tag="PLA17_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HPK85472.1"
                     /translation="MKQKIRFSLVYRDMWQSSGKYQPRVDQLAAVAPHIVEMGCFDRI
                     ETNGGAFEQVQLLYGENPNTAVREWVKPFNKAGIQTHMLERALNALRMYPVPADVRRL
                     MYKVKKAQGVDIARSFCGLNDHRNLELSVKYAREAGMISQAALSITHSPVHTVDYYMD
                     IVDHLVEYGADEICLKDMAGIGRPASLGELVRRIKSKYPGIIVQYHGHSGPGMSVASM
                     LEVARAGADYLDVAMEPLSWGMVHPDVIAIHAVMSDAGFDVPEINMDAYMKVRTLTRE
                     FINDFLGLFIDPRNRQMTSLLIRSGLPGGMMGSMMADLKGVHQGINAALKARNKPEMT
                     EDELVVELFREVGHIWPMMGYPPLVTPFSQYVKNVALMNIVSLVNGKERFAMIDPNTW
                     DMLLGRAGTLPGPLAPELIELARKEGKEFYEGNPQDNYPDALDTYRKEMEENGWPLGP
                     DDEELFELAMHDRQYRDYKSGMAQERFLKEIADKQAELSKVAPAKMPRRKLLLSPETG
                     TAFLYSGETATYWPEEEMINPGEKVRKGEIVMYIMIGKTIREVVSDRAGTVKKILVRS
                     GDKVNKGDPLIEFS"
     gene            2517..3317
                     /locus_tag="PLA17_08390"
     CDS             2517..3317
                     /locus_tag="PLA17_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPK85473.1"
                     /translation="MKGLVFSIKRYAIHDGPGIRVTLFMKGCPLDCWWCHNPEGKEKG
                     IRMVERVDRVGEKEFRSMEQVGHEYTPEEILAKVRADQVFMEHSGGGVTFSGGEPLMQ
                     PEFLLETLTLLKKAGFHTTVDTSGHVRREILRSIMPVTDLFLYDIKHTDPEIHRKFTG
                     VSNELIISNLDMLLAEGKDVMIRIPVIPGVSADAAYMEQLRGFIESRNNGHIKEINLL
                     PYHKTGSSKYRRFGMDDRMASVTQVSNGQLDNYIDILKPLGIHVKKGG"
     gene            3354..5717
                     /locus_tag="PLA17_08395"
     CDS             3354..5717
                     /locus_tag="PLA17_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HPK85474.1"
                     /translation="MTDRVMRLREQSLNATETITAERARLITRFYASREAQGLSAPLL
                     RARAFEYLLRNKAICINEGELIVGERGPAPKATPTYPEISLHSLQDLDILNSREKVFF
                     RVSDEVKKIYSEEIIPFWKGRSNRDRIMDRMTPEWLAAYKAGIFTEFQEQRAPGHTVL
                     GYKMFHTGFLDLKKEISAAAAALDFHNDPQAYERREELKAMDIACDAIIMYAGRHADR
                     LEEMASEEKDSVRRAELQKMAAICRRVPANAPETFHEMLQHYWFIHLGVVTELNPWDS
                     FNPGRLDQHLWPVYNKGVESGTLTPEEAYELLGCFWVKFNNHPSPPKMGVTAQESNTY
                     TDFCLINLGGVKPDGTNAVNPMTYILLDVIQEMRILQPSSMIQVSRKNPDSFVHKALD
                     IIRTGFGQPSVFNTEAIIQELLRQGKSLVDARNGGASGCVETGAFGTECYWLTGYFNL
                     VKILEVTLNNGFDPRTGVQVGPKTGDPASFSTFDEFMMAYKEQINYFADVKIRGNNVI
                     EKTFAIHLPVPFLSLLLEDCIAKGRDYNAGGARYNTTYIQGVGLGSITDILTSIRFNI
                     YDRKRFTWDELLTALKNNFEGAEQMQYDIIYNTPKYGNDDDYADAQAVSVFEFFYDAV
                     NGRPTPRGGTHRINMLPTTSHVYFGNVTGATPDGRKAWVPLSEGISPFQGVDRQGPTA
                     VIKSASKIDHLRTGGTLLNQKFSPEFFEDEGSYQKLTALIRSYFSLDGHHIQFNVVSA
                     DTLREAQKHPELYRDLIVRVAGYSDYFNDLGEDLQNEIIRRTEHQSF"
     gene            5885..6583
                     /locus_tag="PLA17_08400"
     CDS             5885..6583
                     /locus_tag="PLA17_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPK85475.1"
                     /translation="MREIFDYFDARFRADMLGRPVDQTGPVITISRQTGCDGREVAAE
                     LVAQMNLRYGSNRWKWVDKDIIYHAAHELKTDTQRVETYYHGQRMTDISQMIMAFSGS
                     FVTDSSVRKAVRDVVLSIARDGYAVIIGRGSVSITRDMANALHVRLVAPHPWRVRNVM
                     KKKEMTIEKAEEYVARTDANRQNMIVDFLDSKPQSLDHLFDVTLNRSSFTLEQISSMI
                     LSMYEMKLGLKPQR"
     gene            complement(6678..>7188)
                     /locus_tag="PLA17_08405"
     CDS             complement(6678..>7188)
                     /locus_tag="PLA17_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPK85476.1"
                     /translation="SEMLSTMAPDPIVFAIANPDPEISYEEALATRDDLIFATGRSDY
                     PNQINNLLGFPYIFRGALDVRARTINREMKLAAARALAGLAREKVPEAVLRAYGLENL
                     EFGRAYIIPKPLDPRLISRVAPAVARAAMETGVARFHIEDWDAYALRLDSRIREHIKR
                     ISDICCLSV"
BASE COUNT         1807 a         1842 c         1799 g         1740 t
ORIGIN      
        1 cttcagggtt attgatgatg gcttcaacct ctgccatctc ctcttccatc cctttcagga
       61 aggccggttt gaaatgctca aattttatct tgtcaaacgg gggaacttca aaaggagtgt
      121 cccaatcgca atagaacgga ttttcgcagc tctcattctt gcaggaaata accccgagag
      181 agacaatgaa cagcatgata aggatttttt tcattatgat tagtatttag gttcaacagg
      241 ctaaaatatg caataatgtc ttaaaataca atatgaacgg gtgctatcag ccctgttttc
      301 cggcataacc ccggtaaaaa ggctaactga attcaatcag cgggtctccc ttgttgacct
      361 tgtcaccgct cctgacaagt attttcttca ctgtacctgc ccggtcagat accacctccc
      421 tgatggtttt tcctatcata atatacatca cgatctcgcc cttcctgact ttctccccgg
      481 ggttaatcat ctcctcttca ggccagtaag ttgccgtttc tcccgagtag aggaacgctg
      541 tgcctgtctc tggcgaaagc agcagtttgc gccttggcat tttcgcggga gcgacctttg
      601 ataactcagc ctgtttgtcg gcaatctcct tcaggaatct ctcctgtgcc atacctgatt
      661 tgtaatcacg gtattgcctg tcatgcattg ccagttcgaa gagttcttca tcatcgggtc
      721 ccaaaggcca cccgttctct tccatctcct tacggtatgt gtcgagtgca tcaggatagt
      781 tatcctgcgg attaccttca tagaactcct tcccttcttt cctggccagt tcaatcagtt
      841 caggcgccag cgggccggga agggttccgg cacgtcccag cagcatatcc caggtgttgg
      901 gatcaatcat tgcaaatctc tccttgccgt taaccagcga gacgatattc ataagagcca
      961 cattcttcac atactggctg aagggtgtga ccagcggagg ataacccatc atcggccata
     1021 tatgccccac ctccctgaag agttctacca ccagttcatc ctcggtcatc tcaggcttgt
     1081 tcctggcttt cagggcggca ttgattccct ggtgtactcc cttcaggtct gccatcatgc
     1141 tccccatcat tccgccgggg aggcctgacc tgatgaggag ggaggtcatc tgcctgttgc
     1201 gcggatcaat gaacaggccg aggaaatcat ttatgaattc ccgggtcagt gtccttacct
     1261 tcatgtaggc gtccatgttg atctcaggta catcaaagcc ggcatcgctc atcactgcat
     1321 gtatcgcaat cacgtcgggg tgaaccatgc cccagctgag aggctccatt gccacatcaa
     1381 gataatcagc tccggctctt gctacctcaa gcatcgaggc aactgacatg ccgggaccgc
     1441 tgtgaccatg gtactgcaca ataattccgg ggtatttgct cttgatcctt cttacgagtt
     1501 caccaaggct ggcgggtcgg ccaatacctg ccatatcctt gagacagatc tcgtcagcgc
     1561 cgtactccac caaatggtca acaatatcca tataataatc gaccgtatga acaggcgagt
     1621 gtgttattga aagggctgcc tgtgatatca tccctgcctc gcgggcatac ttcactgaca
     1681 gctccaggtt gcggtggtca ttcagtccgc agaatgatcg tgcaatatca accccctgtg
     1741 ctttcttgac cttgtacatc agccttctca catcagcagg caccggatac atcctcaaag
     1801 cattgagagc cctctcaagc atgtgtgtct gaattcctgc cttgttgaat ggcttcaccc
     1861 attcccttac agccgtattc ggattctcac cgtaaagcag ttgaacctgc tcaaatgcgc
     1921 ctccgttcgt ctctatgcgg tcaaagcaac ccatctcaac tatatgcggc gcaacggcag
     1981 ccagctggtc aactctcggc tggtatttgc cagatgactg ccacatgtca cggtacacga
     2041 ggctgaatct tatcttctgt ttcattatct tttgtattat gtagctgccc gcaaatcagg
     2101 gggcggcatt aaagcatcaa agataatccg gtttggttct gcataattga ttattatcac
     2161 aaaaaatgtg aaagagggag aaacctgccc gggacatctt catttcaacc tgaacctgat
     2221 cgctggcagg ctggctgacc ccaaggaatt taccgttgca ggtacacatg actgaaagat
     2281 cgtcagcgat gacaaggtaa ttattgcaat tcttctcctt ttggaagttt cagaatcatt
     2341 tcttaacttt gtcttccaaa ttaccggtat gaacagactg gaacacaggt ttatgatgaa
     2401 ccgtctcggc agaaggctct atagctgcct tatgttctat gcctgatagc ctttcatgag
     2461 gggctcccga agccctgcct ccccgctatt tgttacccta aatccgttct catattatga
     2521 aaggtttggt attcagtata aaacgatatg caattcatga cggccccggc atcagggtca
     2581 ctctcttcat gaaaggttgt ccgctcgatt gctggtggtg tcacaatccg gaggggaagg
     2641 agaagggtat ccgcatggtt gaaagggttg acagggttgg agagaaggag ttcaggtcaa
     2701 tggagcaggt gggtcatgag tacacgccgg aagagattct tgcaaaggtg cgggcagacc
     2761 aggttttcat ggaacactcc ggaggaggtg taaccttctc aggcggcgaa ccactgatgc
     2821 agccggaatt tctgcttgag accctcaccc ttctcaaaaa ggcaggcttc catacaacag
     2881 ttgacacatc aggtcacgtg cgccgcgaga tactacggtc aatcatgcct gtcaccgatc
     2941 tcttccttta tgacatcaag catactgatc cggagataca caggaagttc accggcgtga
     3001 gcaatgaact gataatatcc aaccttgaca tgctcctggc agaggggaag gatgtgatga
     3061 tacgcatccc ggtcataccg ggagtctcag ctgatgcagc ctatatggag cagctccgcg
     3121 gtttcatcga gagccgcaat aacggacaca taaaggagat aaacctgctg ccctaccaca
     3181 agacaggcag ctcaaagtac cgccgtttcg gaatggatga caggatggct tccgtcaccc
     3241 aggtatcaaa cggccaactt gacaattaca ttgacatatt aaaaccgctc gggatacacg
     3301 tcaaaaaggg cggataaaaa gagaatcagg cagaaaaaat attaattatg aaaatgacag
     3361 acagagttat gaggctcagg gaacagagcc tgaacgcaac cgagacaata acggcagaga
     3421 gggcacggct tatcacccgc ttctatgcct ccagggaggc ccagggcctt tctgcacctc
     3481 ttctcagggc acgtgccttt gaatatcttc tgaggaacaa ggcgatctgt ataaatgagg
     3541 gtgaacttat tgtgggcgaa agaggacctg ccccgaaggc tacccctacc tacccggaga
     3601 taagccttca ctcgctgcag gatcttgata ttctcaacag ccgtgaaaaa gtcttcttca
     3661 gggttagtga tgaggtcaag aagatataca gtgaggagat cattcccttc tggaagggaa
     3721 ggagcaaccg tgatcgtatt atggatcgca tgacccctga gtggctggcc gcctataagg
     3781 caggtatttt caccgagttt caggaacaaa gggcgcccgg gcataccgta ctgggctata
     3841 aaatgttcca tacaggcttt ctggacctga aaaaagagat atcagctgcc gccgcagcac
     3901 ttgatttcca caatgaccct caggcctatg agcgcagaga ggaactgaag gccatggata
     3961 tagcctgtga tgcaatcata atgtatgccg gaaggcatgc tgacaggctt gaggagatgg
     4021 cttcggagga gaaggattca gtcaggcgcg cagagcttca gaagatggct gccatatgcc
     4081 gccgcgtgcc ggccaatgct ccggagacat tccatgagat gcttcagcat tactggttca
     4141 ttcacctggg tgtggtgacc gaactcaatc cgtgggactc attcaacccc ggcaggcttg
     4201 atcagcacct gtggcccgta tataacaagg gtgtggagag tggcaccctg actcctgagg
     4261 aggcgtatga actgctcgga tgcttctggg taaaattcaa caaccatccc tcacccccga
     4321 agatgggtgt caccgcacag gagagcaaca cctataccga cttctgcctg ataaacctgg
     4381 gaggagtgaa gcctgacggc accaatgcgg tcaatcccat gacctatatt ctccttgatg
     4441 taatccagga gatgaggata cttcagccca gctcaatgat tcaggtgagc cgcaaaaacc
     4501 cggactcatt cgtgcacaag gcacttgaca tcatcagaac cggattcggg cagccttcag
     4561 tcttcaatac cgaggcaatc attcaggagc ttctcaggca gggcaagagc ctggttgacg
     4621 cccgcaacgg aggagcatcg ggttgtgtgg agacaggggc cttcggcacc gagtgctact
     4681 ggcttaccgg atacttcaac cttgtgaaga tactcgaagt aaccctcaac aacggatttg
     4741 atccacgcac aggcgtacag gtgggaccaa agaccggcga ccccgcctca ttctccacct
     4801 tcgatgagtt catgatggca tacaaggagc agatcaacta cttcgccgat gtcaagataa
     4861 ggggcaataa tgtcatcgaa aagaccttcg ccattcacct gcctgttccg tttctgtcac
     4921 tcctgcttga ggactgcata gcaaagggca gggattacaa tgccggcgga gccaggtata
     4981 ataccacata tatacagggt gtgggcctgg gaagcataac tgacatactc acctcaatca
     5041 ggttcaatat ctatgacagg aagaggttca catgggatga gctgctgacc gccctgaaga
     5101 acaactttga aggagcggag cagatgcagt acgacattat ctacaacacc ccgaaatacg
     5161 ggaatgatga tgactatgct gatgctcagg cagtatcggt atttgagttt ttctatgatg
     5221 ctgtcaacgg caggccgacc cccaggggag gcacacaccg catcaacatg ctgcctacca
     5281 cctcacatgt ttacttcggc aatgttaccg gtgcaacgcc tgacggacga aaggcatggg
     5341 ttcccctgtc agaaggcatt tctccgttcc agggcgttga ccgccaggga cccacagccg
     5401 tgatcaagtc ggcctcaaag attgaccatc tccgtaccgg cgggacactg cttaaccaga
     5461 agttttcacc ggagttcttc gaggatgaag gaagctacca gaagctgacc gcgcttatca
     5521 ggagttactt cagccttgac ggccaccaca tccagttcaa cgttgtcagc gccgacaccc
     5581 tccgtgaggc gcagaaacat cctgagctgt accgtgacct gattgtacgc gtggcaggct
     5641 acagtgatta tttcaatgac ctgggagagg accttcagaa cgagatcatc cggcgtacgg
     5701 aacatcagag cttctgacgg aatcgcccgc agttcctgca tgaccttgcc aaaatgctga
     5761 taaaaatcaa tttttattga ttttcgattg cattttacct gattatttac tattttgtat
     5821 gaattaggtg ctgtggcatt caggcagcaa tacatgcagg tgtacatcac ttaatgcctt
     5881 gattatgaga gagatatttg attatttcga tgcccgcttc agggctgaca tgctcggcag
     5941 gccggttgat cagacaggcc cggtaataac tatctcgcgc cagaccgggt gtgacggtcg
     6001 tgaggtggct gccgaactgg tggctcagat gaatctcagg tacggcagca acaggtggaa
     6061 atgggttgat aaggacataa tctatcatgc tgcacatgag ttgaaaacag atacccagcg
     6121 tgttgagacc tattaccatg gtcagagaat gaccgatatt tcccagatga taatggcatt
     6181 ctcaggttca tttgtgacag acagttcggt caggaaggcc gtcagggatg tggttctctc
     6241 gatagcccgt gacgggtatg cagtgatcat aggcagggga agcgtctcca tcacccgtga
     6301 tatggccaac gccctgcatg taaggctggt ggctccccac ccctggaggg tacggaatgt
     6361 aatgaaaaag aaggagatga ccatcgaaaa ggccgaggag tatgtcgccc ggactgacgc
     6421 caacaggcag aacatgattg ttgatttcct tgacagtaaa ccgcaatcgc ttgatcatct
     6481 cttcgatgtg actttgaaca ggagcagttt cacccttgaa caaatctcat ctatgatact
     6541 ctcaatgtat gagatgaagc ttgggctgaa gcctcaacga taatgctgtg cgttttcaga
     6601 gtttggcaca gcaggctggt tgattacagg agggtaagtg aaagggccgg taacaatggt
     6661 tagcggccct ttctatatca tacactgaga cagcagatgt cagagatgcg ttttatatgc
     6721 tcccttatcc ggctgtcaag ccggagggca tatgcatccc agtcttcaat gtggaagcgt
     6781 gccaccccgg tttccatggc tgcacgggct actgcgggtg caacccttga gataagacgc
     6841 ggatccaggg gtttcggtat tatgtatgcc ctgccgaatt caagattttc aaggccgtaa
     6901 gccctgagca ccgcctccgg aaccttctcc ctggcaagcc cggcaagcgc acgcgctgca
     6961 gcaagcttca tctccctgtt gatggtcctt gccctgacat caagagcacc cctgaaaatg
     7021 taggggaaac cgaggaggtt attgatctgg ttgggataat cagacctgcc ggtggcgaag
     7081 atcagatcgt cgcgggttgc cagggcttcc tcgtaactta tctccggatc gggattggct
     7141 attgcaaaga ctatcggatc aggagccatg gtgctcagca tctcactg
//
LOCUS       DAOPBK010000138         7185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9343_length_7185_cov_5.268864, whole genome shotgun sequence.
ACCESSION   DAOPBK010000138 DAOPBK010000000
VERSION     DAOPBK010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9343_length_7185_cov_5.268864"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..565
                     /locus_tag="PLA17_08410"
     CDS             140..565
                     /locus_tag="PLA17_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK85477.1"
                     /translation="MTFEYKVQGGDFTHAGYASSQVKKMLKQLDVDNVVIKRVVVCLY
                     EAEVNIVAHAWDGVISIVLETDTIFMKLTDNGPGIPNIEQAMQRGFSTASHEVREMGF
                     GAGMGLPNISANADKFLITSEPGKGTTVEITINLNSTAV"
     gene            570..1964
                     /locus_tag="PLA17_08415"
     CDS             570..1964
                     /locus_tag="PLA17_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPK85478.1"
                     /translation="MEKGFFHHALTIREDICTGCSLCMRVCPTEALRIRDGKARLIED
                     RCVDCGECFRNCPVHAIIIEQDDFSRIFDYKYRVALVPAVLHGQFRHEVSMSEISAVL
                     KSLGFTWVYEVEHGAEILKPAIDDLMDMRTLPRPLISSYCPAIVRLIQVRFPALIRNI
                     ATVKAPLEMAAFYCRRVLRDGGAHNAEIGVFYVTPCAAKIAAVKSPVGEESTPISGVI
                     NMDFIYNKINRTLRNKGELKYGDDHAYSVNPETLAWSLTNGEASCINGSTLAIDGMNN
                     VTEFLEQLENDEITGVDYLELRACDEGCAGGVLLSSNRFLVAERLRNKARNLKPGTDS
                     PETEEFKNIEAYREYLMNKVRITEEIMPRSMYRLSGDRDEAMKMMKRSRRLMCWLPGT
                     DCGLCGAPSCQALAEDIVRQRGDISQCVFIRESNMGKNLMDMERSSEIMKRIWGDNNF
                     TKNCNKKGAENESN"
     gene            1951..2283
                     /locus_tag="PLA17_08420"
     CDS             1951..2283
                     /locus_tag="PLA17_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPK85479.1"
                     /translation="MKVTDIVEKLGLNVFSGHEGLGREVTGGYVSDLLSDVMGHAESG
                     DLWLTIQTHRNVMAIASLKDISAVIITSGEKPGDDTIAQSNQEGIPVLGTELSTFELA
                     GRLYSLLN"
     gene            2286..3002
                     /locus_tag="PLA17_08425"
     CDS             2286..3002
                     /locus_tag="PLA17_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HPK85480.1"
                     /translation="MRPFRADLHIHSLLSPCGSLEMSPHEIIVRAKSRGLDMIAVSDH
                     NSTLHCELTVTLGREAGLTVLRAAEVTSAEDVHSLVILPDEEATLSFQEWIDSHCAYM
                     PHNPDIFGDQVVIDRDENIIREIDHFLPAALNASIDEVESAAHSHGALFIPAHIDKPS
                     MSILSQLGFIPDDLFIDAVEVIRSVPNLLFPVIRNSDAHIPEHIGRRYTDYMLENASF
                     DELSKALKGEDGRFIIPLAS"
     gene            2999..3565
                     /locus_tag="PLA17_08430"
     CDS             2999..3565
                     /locus_tag="PLA17_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK85481.1"
                     /translation="MKEIALHILDITQNSIKAGANEIRITLSESIPDDLLSLTIADNG
                     KGMDAESCRRASDPWFTSRTTRKVGLGLPLLKMNAGLTGGGMTIDSEPGKGTTVNATF
                     GYKHVDRPPLGDVSGTIALMILSNPSINIVYSHICEGKEWSISTSEIKEVLGEEAVTD
                     LTIVRHLREIINGNVAEIRNFQTGYDKY"
     gene            3642..4034
                     /locus_tag="PLA17_08435"
     CDS             3642..4034
                     /locus_tag="PLA17_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPK85482.1"
                     /translation="MEKLKSLADLKRIREEAKTKLDLRVRGDNPEGLVQVKVAMATCG
                     IASGAKQTMDFFVEELDKRGIGAVVTQTGCMGYCYAEPTVEVQLPGQESVVFGYVDRK
                     KADEIIEKYIKNGELVEGIIPVNYEKID"
     gene            4055..5848
                     /locus_tag="PLA17_08440"
     CDS             4055..5848
                     /locus_tag="PLA17_08440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPK85483.1"
                     /translation="MSKFRMHLLVCGGTGCRASESNLILESLKRELEEKGLSSEVQVI
                     MTGCFGFCEKGPVVKVMPDNTFYVHVKPEDAGIIVAEHVIKGRPVQRLLYVDPKSKEQ
                     VPDSKHMGFYRKQIRIALRNCGFINPENIDEYIARDGYASLGKCISELTPADVIDIIK
                     KSGLRGRGGAGFPTGLKWEIASKNKADQKYVVCNADEGDPGAFMDRSVLEGDPHSVLE
                     AMTICGYAIGASKGLIYIRAEYPLAIHRLKIALKQSKEYGLLGKNIFGTDFNFDIELR
                     YGAGAFVCGEETALISSMEGRRGEPTNKPPFPAEKGFFGKPSNVNNVETFANIPVIIS
                     KGWEWFSSIGTEKSKGTKVFALAGKINNVGLIEVPMGTTLREIIFEIGGGIKNGKKFK
                     AVQTGGPSGGCLTEKHLDTPIDFDNLVAAGSMMGSGGMIVMDEDDCMVSIAKFFLAFT
                     VEESCGKCAPCRVGNKRLLELLTHITKGNGTMADIDRLKNMCNVIKDTSLCGLGQTSP
                     NPVLSTINNFMDEYVAHVTEKRCPAGSCRELMKYSIIVDKCVGCTACARGCPVNAISG
                     ERKGPHFIDQEKCIKCGACFEKCKFGAVLIK"
     gene            5866..>7185
                     /locus_tag="PLA17_08445"
     CDS             5866..>7185
                     /locus_tag="PLA17_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HPK85484.1"
                     /translation="METIKITIDNKQVEVEKGTTILDAARKMGIDIPTLCHMRLDDLN
                     IENKPGGCRICVVEVQGRRNLAPACITQCVDGAVIRTHSPRVMNARRTVMEFILSDHP
                     KDCLICPKSGNCDLQNMAHRLGIREIPGHEIAEISTYRKDTSPSIIRDLDKCIMCRRC
                     EMMCNEVQTVGAIGAFNRGFMAAVGPAYEQDLEHSPCTYCGQCVAVCPTGALTEVDHT
                     REVLRAIVDPSKTVVVQTAPAVRAALGEEFGMKPGTLVTGKMVTALKELGFDYVFDTD
                     FAADLTIMEEGTELLTRLKKFLNGDKDANIPILTSCCPGWVNFFEHNYPELREVPSTA
                     KSPQQMFGAIAKSYFAEKININRKDMVVVSIMPCLAKKYERQRPEFSVDGNPDVDFSL
                     STRELASFIRQANIDFANLPESEFDRPLGESTGAAVIFGNTGGVIEAA"
BASE COUNT         1956 a         1781 c         1850 g         1598 t
ORIGIN      
        1 aaaatcggtc accccggaaa tgaaaaaact ggccgatgaa ctcggcatga ctctcattac
       61 ctgtcccatg acgatgttca aggcttcagg tttactatac aatgccggaa tcgaacctct
      121 attctgacaa gcagtggcga tgaccttcga atataaggta cagggaggtg acttcaccca
      181 tgccggttat gcttcaagcc aggtaaagaa gatgcttaaa cagcttgacg ttgacaatgt
      241 tgtcatcaag agggttgtag tctgtctcta cgaggctgaa gtcaacattg tggctcatgc
      301 ctgggacggt gtaatcagca ttgtgcttga gaccgacacc attttcatga agcttacaga
      361 taacggcccc ggcattccca atatcgagca ggcaatgcag aggggctttt caactgcatc
      421 acacgaagta agggagatgg ggttcggagc agggatggga ctgcccaata tcagcgcaaa
      481 tgctgacaag ttcctcatca ccagcgaacc tggcaaaggc actacagtgg agataacaat
      541 taatctgaac agcacggctg tttgaaacaa tggaaaaagg gttctttcat cacgcactca
      601 ctatccggga ggatatatgc acaggctgtt cactctgcat gagggtttgt cccaccgaag
      661 cgctacgtat acgtgacggc aaggccaggc ttattgaaga tcgctgcgtc gactgcggtg
      721 agtgcttcag gaattgcccg gtacatgcta ttataatcga gcaggacgac ttcagccgta
      781 tctttgacta caagtaccgc gtggctcttg tgccggcagt gctccacgga cagttcaggc
      841 atgaagtatc catgtcggag ataagcgcag tgctgaagag cctgggattc acatgggtct
      901 atgaggttga acacggagcg gaaatactca aacctgcaat cgatgacctg atggatatgc
      961 ggacactccc ccgccccctg atctcaagct attgcccggc aatcgtacgc ctcatacagg
     1021 tgcgcttccc tgcactgatc aggaatattg ccacagtgaa ggcaccgttg gagatggctg
     1081 ctttctactg ccgcagggtg ctgcgtgacg ggggcgccca taacgccgag ataggcgtat
     1141 tttatgttac cccctgcgcc gccaagatag cagccgtaaa atcacccgtg ggtgaagaat
     1201 caacccccat cagcggcgta atcaacatgg acttcatcta caacaagata aaccgcacac
     1261 taagaaacaa gggtgaactg aaatacggcg atgaccatgc ctattccgtt aaccctgaaa
     1321 ctcttgcatg gagcctcacc aatggtgaag cctcctgtat caacggcagc accctggcta
     1381 ttgacggaat gaacaacgtc actgaattcc tcgaacaact cgaaaacgat gagatcaccg
     1441 gtgttgatta tctcgaactg agggcctgtg atgagggttg tgccggcggt gtgctcctct
     1501 cctccaatcg tttcctggtt gccgaaaggc tccgtaacaa agcccgtaac ctcaaaccag
     1561 gcacagactc tccggaaacg gaagagttca aaaacattga agcatacaga gaatacctga
     1621 tgaacaaggt gagaatcacc gaggagatta tgccccgttc gatgtaccgc ctctccggag
     1681 accgcgatga ggccatgaaa atgatgaaac ggtcacggcg gctcatgtgc tggctcccgg
     1741 gaaccgactg cggcctctgc ggggcgccat catgccaggc actggctgaa gatatagtcc
     1801 gtcaacgggg tgatatctca cagtgcgttt tcatccgcga gtcaaacatg ggaaagaatc
     1861 tgatggacat ggaaagatca tcagaaataa tgaaacggat atggggagat aacaatttta
     1921 caaaaaactg caataaaaaa ggtgctgaaa atgaaagtaa ctgacattgt tgaaaagctt
     1981 ggacttaatg tattctctgg gcatgaaggc ctgggcaggg aggtaaccgg cggctatgtt
     2041 tccgaccttc tgagtgatgt gatgggacat gccgaatcag gagatttgtg gctcactatc
     2101 cagactcacc gtaacgtcat ggcaattgcc tcactcaaag atatttcagc tgtaattatc
     2161 acctcagggg aaaaaccggg tgatgataca atagcccaga gcaaccagga aggcattccg
     2221 gtgctgggaa ccgaactgtc aacatttgaa cttgcaggca ggctttattc actattaaac
     2281 tgattatgag accgttcagg gctgatcttc atattcattc cctgctctct ccctgcggct
     2341 cacttgagat gagcccgcat gagattattg tacgggcaaa gagcagggga ctggacatga
     2401 tagcagtctc tgaccacaac agtacattgc actgtgagct gacggtcacc ctcggccgcg
     2461 aggccggcct tacggttctg agggccgctg aggtcaccag cgctgaggat gtgcactctc
     2521 tggtgatcct cccggatgag gaagcaaccc tatcgttcca ggagtggatt gacagccatt
     2581 gcgcttacat gccacataat cctgacatct tcggtgacca ggtagtgatt gacagggatg
     2641 agaacattat ccgtgagata gaccattttc tgccggctgc cctgaatgcc tccatcgatg
     2701 aggttgagtc agcagcccac agccatggag ctcttttcat cccggcacat attgacaaac
     2761 cctcaatgag catcctgagc cagctcgggt ttatccccga tgaccttttc attgatgcgg
     2821 tggaggttat tcgcagtgtg ccaaaccttc tgttcccggt tatcaggaat tccgatgctc
     2881 atatcccgga acatatcggg agacgataca ctgattatat gctcgaaaac gcctccttcg
     2941 acgaactgtc aaaggccctg aaaggtgaag atggaagatt cattatacca cttgcgtcat
     3001 gaaggagata gcacttcata ttctcgacat tacccagaac tcaatcaaag ctggcgccaa
     3061 tgaaatccgg atcacactca gtgagtcgat acctgatgat ctcctttcac tcaccatagc
     3121 tgacaacggc aaggggatgg atgcggagag ctgccgcagg gcttccgatc catggttcac
     3181 ctcgcgcacc acgcgaaaag tgggtttggg cctgccgctg ctcaagatga atgccggcct
     3241 caccggaggc gggatgacaa tcgattctga acccggaaag ggaacaacgg taaatgccac
     3301 cttcggctac aagcatgtag acagaccgcc gctgggtgat gtgagcggaa ccattgcgct
     3361 gatgattctg tcaaacccct ccataaatat tgtctattcc cacatatgtg aagggaagga
     3421 atggagtatc tccaccagtg agataaaaga ggtcctgggg gaggaggcgg tcacagacct
     3481 gaccattgtc aggcatctca gggaaatcat aaatggaaat gtggctgaga tcagaaattt
     3541 tcaaacggga tatgacaaat attaggtatt aagtaactta ttgaagttag atttgcgaat
     3601 taaccataac ttacatctaa gtcagaatta aaatatttga aatggaaaaa ctaaaatcac
     3661 ttgcagactt aaagaggatt cgcgaggaag caaagacaaa gcttgacctg agggtaaggg
     3721 gtgataaccc cgagggactc gtccaggtta aggttgcaat ggctacctgc gggatcgcat
     3781 caggtgcgaa gcaaacaatg gatttctttg ttgaagagct tgacaagaga ggcattggcg
     3841 ccgttgtcac ccagacgggt tgcatgggtt actgctatgc cgaacctacg gttgaagttc
     3901 agctcccagg ccaggaatct gtcgtttttg gctatgttga cagaaaaaaa gctgatgaga
     3961 tcattgagaa atacatcaag aatggtgaac tggttgaagg catcataccg gttaactatg
     4021 aaaaaataga ctaataacag ggaggaaaca gataatgtca aaattcagaa tgcaccttct
     4081 tgtctgtggc gggactggct gtcgtgcctc agaaagcaat ctcatccttg agagtctgaa
     4141 gagggaactt gaagagaaag gcctcagcag tgaggtacag gttataatga ccggctgttt
     4201 cggtttctgc gagaagggac cagttgtcaa ggttatgcct gataacacct tctatgtgca
     4261 tgtcaagcct gaggatgcag gaattattgt tgccgagcat gtgattaagg gcaggcccgt
     4321 acagcgcctg ctgtatgttg acccgaagag caaggaacaa gttcccgatt caaagcacat
     4381 gggtttctac cgcaagcaga tccgtatagc cctgcgtaac tgcggtttca tcaaccctga
     4441 aaacatagat gaatatatcg cccgtgacgg gtatgcttcc cttggcaagt gcatcagcga
     4501 gctcaccccg gccgatgtca ttgacataat aaagaaatca ggccttcgcg gacgtggcgg
     4561 tgccggtttc cccaccgggc tcaagtggga gatcgcttca aagaacaagg ctgaccagaa
     4621 gtacgtggtc tgcaatgctg acgagggtga cccgggtgca ttcatggacc gttcggttct
     4681 tgaaggtgac ccccactcag tccttgaggc aatgaccatc tgcggttatg ccatcggcgc
     4741 atcgaaagga ctcatctaca tcagggctga atatcccctg gcaatacatc gtcttaagat
     4801 agccctgaaa cagtcaaagg aatatggcct cctcggaaaa aacattttcg gcactgactt
     4861 caattttgac atcgaactac gctacggtgc cggagcattt gtctgcggtg aggagacagc
     4921 gctgatcagt tcaatggagg gaagacgcgg tgaacccacc aacaaaccac ccttcccggc
     4981 agaaaagggt ttctttggca aaccctcaaa cgtgaacaac gttgagactt tcgccaacat
     5041 ccccgtgata atatccaagg gatgggaatg gttctcttca atcggaactg aaaaatccaa
     5101 gggaacgaag gtattcgccc ttgcaggcaa gataaacaac gtgggactca ttgaagtacc
     5161 catgggcaca accctccgcg aaatcatctt cgagataggc ggtggcatca agaacggcaa
     5221 aaagttcaag gctgtccaga ccggaggccc ttccggcggc tgtcttaccg agaaacacct
     5281 tgacaccccg attgactttg acaacctggt ggctgcagga tcaatgatgg gatcaggcgg
     5341 tatgatcgtc atggacgaag atgactgcat ggtttcaatc gcaaaattct tccttgcctt
     5401 tacagtggaa gagtcatgcg gcaagtgtgc tccatgccgt gttggaaaca agaggctcct
     5461 cgaactcctc acccatatca ccaagggtaa cggcaccatg gctgacattg accgcctgaa
     5521 gaatatgtgc aatgtcatca aggatacttc actgtgcggg ctgggccaga cctctccgaa
     5581 ccccgtgctc tcaacaatca acaacttcat ggatgaatat gtggctcacg tcactgagaa
     5641 gaggtgcccg gccggttcat gccgtgaact tatgaagtac tcaatcatcg ttgacaaatg
     5701 cgtcggctgt accgcctgcg ccaggggatg ccctgtcaat gccatcagcg gtgaacgtaa
     5761 aggaccgcac ttcattgacc aggagaaatg catcaaatgc ggagcctgct tcgagaaatg
     5821 caaatttggt gctgtgctaa tcaaataata ccaggaagag ttacgatgga aacaataaaa
     5881 attacaatag acaacaaaca ggttgaagtc gagaagggaa ctaccatcct cgatgctgca
     5941 aggaagatgg ggatcgatat ccccaccctc tgccacatga ggcttgatga ccttaacatt
     6001 gaaaacaaac ccggcggttg caggatatgc gtcgtggaag tccagggacg caggaacctc
     6061 gctcccgcat gtatcacaca atgtgttgac ggtgctgtca tacgcactca cagcccgagg
     6121 gtgatgaacg cccgcaggac cgttatggaa ttcatactct cagatcaccc gaaggattgc
     6181 ctcatttgtc cgaagtcagg taactgtgat ctgcagaata tggctcaccg gcttggcatc
     6241 agggagattc ccggccatga aatcgcggag atatcaacct acaggaaaga tacctctcct
     6301 tcaatcatcc gtgatcttga caagtgcatc atgtgccgcc gctgtgagat gatgtgcaat
     6361 gaggtccaga ccgtcggtgc aataggcgct ttcaacagag gcttcatggc tgccgtgggc
     6421 cctgcttacg aacaggacct cgagcactca ccctgtacct attgcggaca gtgcgtggca
     6481 gtgtgcccca ccggtgccct tacagaggtg gaccatacgc gcgaagtact gcgtgctatc
     6541 gtcgacccct ccaagacagt ggttgtgcag acagcccctg cagtacgcgc cgccctcgga
     6601 gaagaatttg gcatgaaacc cggaactctc gttacaggca aaatggtgac ggcactgaag
     6661 gaactcggat tcgactatgt gtttgatacc gactttgctg ctgacctcac catcatggaa
     6721 gagggaacag aattactcac aagactgaag aagttcctga acggcgacaa ggatgccaat
     6781 attcccattc ttacttcatg ctgcccgggc tgggtcaact tcttcgaaca caactatccc
     6841 gaactcaggg aggtgccatc aacagccaaa tccccgcagc agatgttcgg cgcaattgcc
     6901 aagtcttact tcgctgaaaa gatcaatatc aaccgtaagg atatggtggt ggtctcaatc
     6961 atgccatgcc ttgccaagaa atatgagaga cagagaccgg agttttccgt cgatggcaac
     7021 cctgatgttg acttctcact ctcaacgcgt gagctggcca gtttcatccg ccaggcaaac
     7081 attgactttg caaacctccc cgaatctgaa tttgacagac cgctcggcga atctaccggg
     7141 gcagccgtca tcttcgggaa caccgggggc gtcattgaag ctgct
//
LOCUS       DAOPBK010000139         7153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9400_length_7153_cov_5.478867, whole genome shotgun sequence.
ACCESSION   DAOPBK010000139 DAOPBK010000000
VERSION     DAOPBK010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9400_length_7153_cov_5.478867"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLA17_08450"
     CDS             complement(<1..407)
                     /locus_tag="PLA17_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPK85485.1"
                     /translation="MFRPELSDEENMIVCGKCSNHLWHTHNYLLMVAVKGEPHTATGY
                     FINATRHKEIMKDRVIVKFDKKNMNLKMDFMTDKAATGKKLAITIWDEPEIPLAEEGA
                     TPHCVRMEEGATLYCMRTEEGATLHCVRTEEGAT"
     gene            535..1203
                     /locus_tag="PLA17_08455"
     CDS             535..1203
                     /locus_tag="PLA17_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85486.1"
                     /translation="MNDAFRNILIAVAHPSEEQVVSVAASHAGMSCEIIVTGVGGAAM
                     SWALQKRFASGDHPSLVMGAGLAGSYVESIRPGAVVTTVSDCFADMGIDDNGRFVPLF
                     GAGLADPDMPPFSGGRIHCSGRGFDLAAGLMPAVRGATVNMVSGSQSVIDRICRAWDP
                     DIETMEGAWLAYTCVMSGIPWIAVKAISNMVEPRNMKNWKIRMALGRLQEKMTLLFEL
                     MVKS"
     gene            1200..2030
                     /locus_tag="PLA17_08460"
     CDS             1200..2030
                     /locus_tag="PLA17_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HPK85487.1"
                     /translation="MKLTLGFSPCPNDTFIFEAMVHGRVDTEGLEFDWFLADVEELNR
                     RAVEGSVDITKMSFHAYARTAHKYLILDSGSALGRSNGPLVVSRRAVRPDELDHALIA
                     IPGKYTTANLLFSIFWPGATRKQEYLFSDIPGAVLSGEADAGLIIHETRFTYSSMGLT
                     MVADTGAMWEKMTGLPVPLGGIVISRSIDHLVASKVERAIRRSIEYARSAPQESVDFI
                     RHHARETDTEVTREHIKLYVNDFSLSLGEEGRRAIEKLFAMAVEKGVANPLPASIFLK
                     "
     gene            2089..2682
                     /locus_tag="PLA17_08465"
     CDS             2089..2682
                     /locus_tag="PLA17_08465"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPK85488.1"
                     /translation="MKFELPPLKYEAGDLAPKLSAETLDYHHGRHHLAYVNNLNNLLP
                     GSPFEEATLETMIRKAEGGIFNNAAQVWNHTFYFASLSPTGKRTPSGTIAKAIDAEYG
                     SFEGFREAFTKAATTLFGSGWAWLCMKPDGRLLITQESNAGNPIRSGLVPLMTCDVWE
                     HAYYIDYRNRRPDYIKAFWELLDWKVIGERYDEALKK"
     gene            2815..3387
                     /locus_tag="PLA17_08470"
     CDS             2815..3387
                     /locus_tag="PLA17_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPK85489.1"
                     /translation="MNFHDLIEKTRSYRRFDGQHEITADTLVSLVNLARLSASAANRQ
                     PLKYILVTDPAQREALFPCLAWAGYLTTWEGPGSGERPTAYIVILGDREISESFGVDH
                     GIAAQSIMLGATEAGLGGCIIAAIKKERLRTAFDIPEQYEILLVLALGKPAEEVVIET
                     MRNNDVKYWRDDKSVHHVPKRTLDGIILRR"
     gene            complement(3437..4348)
                     /locus_tag="PLA17_08475"
     CDS             complement(3437..4348)
                     /locus_tag="PLA17_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPK85490.1"
                     /translation="MFNKFIASILPYFPKKFIWIFSRAYISGVTMADAMRVSRELNAL
                     GIKVTLDVLGEFITSLDEAEANKQEYLDLIDETHKNGIDGNYSLKPTSFGLLLDKEEC
                     YRIMREIVAKAASYNNFCRIDMEDSPCTDLEIELYRRLHAEFPRNVGLVLQAYLKRTY
                     SDLEKMLDMNTPEIPTNYRLCKGIYVEPAEISYKKYEEINKHYLEDLEFMFRNKIFVG
                     IATHDKPLIEGAYELIKKYNVPKNMYEFQMLYGVTPKLRQSIVDAGHDMRVYVPFGEK
                     WFSYSTRRLKENPKMASHIMKAIFVKG"
     gene            complement(4490..>7153)
                     /locus_tag="PLA17_08480"
     CDS             complement(4490..>7153)
                     /locus_tag="PLA17_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK85491.1"
                     /translation="GIMLNSTIWDILEGNRIAAKNMLTGEIFYVDAQYMIVATGAIPF
                     MPAFGNDDLPGVYTAAVIQKMMNLEFTLLGRNILTVGAGNIGYLTSYQLMQAGAKVKA
                     IIEAMPHEGGFPVQANRVRRLGIPVILSHTLVRAIPNSTNDGICGAVIAECRNFTPVP
                     GTEKVIEGIDAINICTGLIPDNQLLIKGREVFGTKVFAAGDSIRIGEGTSAVLRGKQA
                     AMEIIQEMGVRSNYNEYLSLSKEYIDSQQHPTRVLENPRMPDEARMKARPFVIIDCLY
                     SFACNPCQFACPHGAITKASPDTVPAIDYEKCTGCLSCVYQCPGLAIFGYNTVKNQLF
                     LPVEYFVEEGSEVWLVDNNGKKIGEGVIEKVMMKPNRTNVARVRAITPEGNDIVSARG
                     FIAKENYPEPLTFTPAATMEAKEYVCHCEDVKLEEIMAVIGDRKYISFDEIKHTTRLG
                     MGPCRGKRCIKRLSMLLKPAGIEIIGDATPRAPLSNQITMGELLPQDTPEIIITRGNV
                     RKVKVAALVAGGGITGSALMRYLAEAGMKPVLINHDRGSSWRNIAGGRPNFSVPELAE
                     LAKHNHELFKELQKIGEINYREIKYVTFAHDEAMMNQLEASLTWSDGEMVMPADYAVK
                     IAPGINPELKKTYRAAMLTNDCWQATPGKVIDLIRHLGIKAGGTVEEDSTVTAVRRNG
                     REYIVIMQNHRGEYVEYETPVFINAMGAQGEKFARSLGLYTGTYGVRHQAFITRRLPM
                     MGPGNTPLPMLIDRRNYKGFIAVYGQQLGETGQIIGCASPAVEPAEAGRNLKINSKEF
                     IEIVSEVFTLWLPELSTAGFQSLWSGYYTEPRMYIDPEHGLFLGLRGQGFMMGQYLAK
                     LYVDKLMGREVPAYFSRLSLKGDGLPETAFI"
BASE COUNT         1575 a         1870 c         1830 g         1878 t
ORIGIN      
        1 gtggctccct cctctgtcct cacgcaatgt agcgtggctc cctcctctgt cctcatgcaa
       61 tacagcgtgg ctccctcctc catcctcacg caatgcggtg tggctccctc ctccgccagt
      121 ggaatctctg gctcatccca tattgttatg gcaagttttt tgcctgtggc tgccttgtct
      181 gtcatgaagt ccatcttcag attcatattc tttttgtcga actttacaat gacccgatcc
      241 ttcattatct ccttatgcct ggtggcatta atgaaatacc ctgttgcagt atgcggttcg
      301 cctttcacag ctaccatgag gaggtagtta tgcgtgtgcc acaggtgatt ggaacacttg
      361 ccgcagacaa tcatgttctc ctcatcgctg agctcagggc ggaacattct gtgcgcggca
      421 ctgtattgct ctcttctcgt caggcaggtc atatccgcta aatcagatgg tgtacctggt
      481 caggaaattt tttgttcaaa aataatatat ttatcgctcc aacttgtctt acctatgaac
      541 gatgcattca ggaacattct cattgcggtg gctcacccat ccgaggagca ggtggtaagt
      601 gtcgcagcgt cgcatgccgg catgagctgt gagataattg ttaccggggt tggaggggct
      661 gctatgtcat gggcgctgca aaagagattt gcttccggcg atcacccctc actggtgatg
      721 ggagccggcc ttgcaggcag ctacgtggag tcaatccggc cgggagctgt tgtcaccaca
      781 gtctctgact gcttcgccga catgggtatt gatgacaatg gcaggttcgt tcccctgttt
      841 ggtgcaggtc ttgctgatcc tgacatgcca ccgtttagtg gcgggcggat ccactgcagc
      901 ggcagagggt ttgatctggc tgccggactg atgcccgctg tcaggggtgc aaccgtcaac
      961 atggtctctg gctcacagtc agtcatagat cggatctgcc gtgcatggga cccggatata
     1021 gagacaatgg agggcgcctg gctggcttat acctgtgtaa tgtcagggat accatggata
     1081 gcggtaaaag ccatatcaaa catggtggag ccccggaaca tgaagaactg gaagatcaga
     1141 atggctcttg gcagactgca ggagaaaatg accctgctct ttgaactgat ggtaaaatca
     1201 tgaaactgac acttggtttt tccccatgcc ccaatgacac cttcatcttc gaggcaatgg
     1261 tgcacgggag ggtagatacg gaggggctgg aattcgactg gtttctggct gatgtcgaag
     1321 aacttaatcg cagggctgtg gagggcagcg ttgacatcac caagatgagc ttccacgctt
     1381 atgcccgcac ggcccacaaa tacctgatac ttgattccgg cagtgccctg ggacgcagca
     1441 acggcccgtt ggtggtgagc cgcagagctg tcaggcctga tgagcttgac catgccctca
     1501 ttgccattcc cgggaaatac accacagcaa acctcctctt cagtatcttc tggcccggag
     1561 ccactcgcaa acaggaatac ctcttttcgg atatccccgg ggcggtgctc tccggagaag
     1621 ccgatgcagg gctgatcatt cacgagaccc gtttcaccta cagttccatg ggtctgacaa
     1681 tggtggctga caccggcgcc atgtgggaga agatgaccgg tctgccggtc ccgctgggcg
     1741 ggatagtgat cagccgaagc attgatcatc ttgttgcgtc aaaagttgag agggccatac
     1801 ggcgaagcat cgaatatgcc cgatccgccc cgcaggaatc ggttgatttc atcaggcatc
     1861 acgcccgcga gactgacact gaggtcaccc gcgaacacat aaagttatat gtcaatgatt
     1921 tcagcctgtc actgggagag gaggggcgcc gggccattga aaagctgttt gccatggccg
     1981 tggaaaaagg cgttgcaaac ccgcttccgg caagcatatt tctgaaatag cagaaaaaat
     2041 caacctattt tagtatcttt acagacaaat ctaaaatttg tccggattat gaaatttgaa
     2101 ctacctccgt tgaagtatga agcaggtgac cttgcgccga agctgagtgc ggaaacgctt
     2161 gattatcatc atggcaggca tcatcttgcc tatgtcaata accttaataa ccttcttccc
     2221 ggctccccct ttgaggaggc tacactcgag acgatgatcc gaaaggctga aggcggtatt
     2281 ttcaataacg ccgcacaggt ttggaatcat actttttatt ttgcatcact atcgccgaca
     2341 ggcaaacgaa caccttcggg aaccatcgcc aaagccattg atgccgaata tggttctttt
     2401 gagggtttca gggaagcttt caccaaggcc gctacaacgc tctttgggtc ggggtgggca
     2461 tggctctgta tgaagcctga tggccgcctg ttgataaccc aggaatcaaa tgccggaaac
     2521 ccgatcagat caggactggt gccgcttatg acgtgcgacg tgtgggagca tgcctattat
     2581 attgattaca ggaacaggcg tcctgactac atcaaggcat tctgggaact gctcgactgg
     2641 aaggtgatag gagagcggta cgatgaagcg ctgaagaaat agactcaggt caggctgctg
     2701 tcaatttata atcaggcagt ctgttgcagg tgatattatc tggcagcccc ggcaaggggc
     2761 tgttttgttg aatcagcatt ttctttttct ctatctttat tcaaaaatcc gactatgaac
     2821 ttccatgacc tgattgaaaa gacacgttcc tacagacggt ttgacggtca acatgagatc
     2881 actgccgaca ccctggtctc tcttgtcaat ctggcccgcc tgtcggcctc cgctgccaat
     2941 cgtcagccac ttaagtacat tctcgttacc gatcctgcac aacgagaagc tctgttccca
     3001 tgccttgcgt gggccggcta tctgaccacc tgggagggac ctggcagcgg agagaggcca
     3061 acggcataca ttgtgatact gggtgacagg gagatatcag aatcttttgg cgttgaccat
     3121 ggtattgcag cacagagcat tatgctcgga gccacggagg caggcctggg aggttgcatc
     3181 attgcagcca taaagaaaga gagactgcga actgcctttg atatcccgga gcagtatgag
     3241 atactgcttg ttctggctct aggcaaaccg gcagaagagg tggtaatcga gaccatgcgc
     3301 aataatgatg tgaaatactg gagagatgac aaaagcgttc accatgtccc gaagaggaca
     3361 cttgacggga tcattctccg caggtagatc cgccaggcca atactgttgc ctgccggcta
     3421 ttgtcttttc tgacaatcag cctttcacaa aaattgcctt cattatgtgg cttgccatct
     3481 tcggattttc cttcagcctc ctggttgaat agctgaacca tttttctccg aacgggacgt
     3541 agactctcat atcatgaccg gcgtcaacaa tgctctggcg cagcttcggg gttactccgt
     3601 agagcatctg gaattcatac atgtttttgg gcacattgta tttctttatc agctcatatg
     3661 ctccctcaat cagcggcttg tcatgagtag caatgccgac aaatatcttg ttccggaaca
     3721 tgaactcgag gtcttcaaga tagtgtttgt tgatctcctc gtacttcttg taggagatct
     3781 cagccggttc cacgtagatg cccttgcaga gacggtagtt ggtgggtatc tccggtgtgt
     3841 tcatgtcaag catcttctcc aggtcactgt atgtccgctt caggtatgcc tgcagcacca
     3901 gccccacatt acgcgggaat tcggcatgaa gacgcctgta aagctcgatc tccaggtcag
     3961 tgcagggaga gtcttccatg tcaatcctgc agaagttgtt gtatgatgct gcctttgcca
     4021 ctatctcacg cataatacga tagcattctt ccttgtcaag aagcaatccg aagcttgttg
     4081 gcttgagcga atagttgcca tcaatcccgt ttttatgggt ctcatctatc aggtcaagat
     4141 attcctgctt gttggcctca gcctcatcaa gcgatgtaat gaattctccc agtacgtcga
     4201 gggtcacttt gataccaagc gcgttcagct cacgtgagac ccgcatggca tcagccatgg
     4261 tcactccgga aatataggca cgtgaaaaga tccagatgaa tttcttcggg aaatagggca
     4321 ggatggatgc aataaacttg ttgaacataa ttgagagttt taaatattaa ttatttgtgg
     4381 ttgatgctta aagatataaa aaaggttaat atctgatatc ctttttgatt atctggtttc
     4441 ctgacatata acaagaatca cctgtaattt tttttactgc ctgtctgttt tatatgaaag
     4501 ccgtttccgg cagcccgtcg cccttgagtg acagcctgct gaagtatgca ggtacttcac
     4561 gccccatcag cttgtctacg tagagttttg ccaggtattg tcccatcatg aatccctgcc
     4621 cgcgcagccc gaggaagagg ccgtgttcag gatcgatgta catgcgcggc tcagtgtagt
     4681 aaccgctcca gagcgactgg aatcctgctg tcgagagctc gggtagccat aaggtgaata
     4741 cctctgacac gatctctatg aactccttgc tgtttatctt caggtttctt cctgcttcgg
     4801 cgggttcgac tgctggtgac gcacatccga ttatctgacc tgtctcaccc agctgctgtc
     4861 cgtatactgc tatgaatccc ttatagttac gccggtctat cagcataggc aacggtgtat
     4921 tgcccggtcc catcatcggc agtcgcctgg tgatgaaagc ctggtgcctg acgccgtagg
     4981 tgccggtata taacccgagg ctgcgggcga atttctcacc ctgcgcgccc atggcattga
     5041 tgaagacggg ggtctcatac tccacgtact cgccccggtg gttctgcata attacgatat
     5101 attcgcggcc gttccgtctc actgccgtga cggtgctgtc ttcttcgacg gtgcctccag
     5161 ccttgattcc caggtgtctt atcaggtcaa tgacctttcc gggtgttgcc tgccagcaat
     5221 cattggtcag cattgccgcc ctgtaagtct tcttcagttc cgggttgatt cccggtgcaa
     5281 tcttcaccgc atagtcagcc ggcataacca tctcgccgtc tgaccaggtc agtgaggcct
     5341 cgagctggtt catcatagcc tcgtcatgtg cgaaggtcac atatttgatc tccctgtagt
     5401 ttatctcgcc aatcttctga agctccttga acagctcgtg gttatgtttt gccagttcag
     5461 ccagttcggg tacgctgaag ttgggtctcc ctccggcaat gttgcgccat gatgatccgc
     5521 ggtcatggtt tatgagaacg ggcttcatcc cggcctcggc cagataacgc ataagggcgc
     5581 tgccggttat gcctccgccg gcaaccagcg ctgccacctt caccttcctt acgttacccc
     5641 gggtgattat tatctcaggg gtatcctgcg gaaggagttc acccatggtg atctggttcg
     5701 agagaggggc cctgggagtg gcatcaccta ttatctctat gcctgccggt ttgagaagca
     5761 tcgagagcct ttttatgcac cgcttgccgc ggcagggccc catgccaaga cgcgttgtat
     5821 gcttgatctc atcaaatgat atgtatttcc tgtcgccaat gactgccatt atctcttcca
     5881 gctttacatc ctcgcagtgg cagacatact cctttgcctc catggtggca gcaggggtga
     5941 aggtcaaagg ttccggatag ttctccttgg cgatgaatcc cctggcgctg acaatatcgt
     6001 ttccttccgg tgtaatagct cttacccttg caacatttgt cctgttgggt ttcatcatca
     6061 ccttctctat tacaccctcg ccgattttct tgccgttatt gtcaacgagc cagacctcac
     6121 tgccttcttc cacgaaatac tccaccggca ggaagagttg gttcttcacg gtattgtatc
     6181 cgaagatagc cagccccggg cactggtaga cacatgacag gcaccccgtg cacttttcat
     6241 agtctattgc cggtaccgtg tcaggcgatg ccttcgttat tgccccgtgg gggcaggcga
     6301 actggcacgg gttgcaggca aaactgtaaa ggcagtcgat tatcacgaac ggtctcgcct
     6361 tcatccttgc ctcatcgggc atcctcggat tctcaaggac ccttgtcggg tgctgctgtg
     6421 aatcaatata ttcctttgat aatgaaagat attcattgta gttgctgcgt acgcccatct
     6481 cctggataat ctccatggcg gcctgctttc cgcggagcac tgccgaggtg ccctcgccaa
     6541 tccgtatgga gtcacctgcg gcaaacacct tcgtgccgaa cacctcgcgg ccctttatca
     6601 ggagctggtt gtcggggata aggccggtac aaatattgat ggcatcaatg ccttcgatga
     6661 ccttttctgt tcccgggaca ggggtgaagt tgcggcactc ggctatcact gcaccgcaga
     6721 tgccgtcatt ggtgctgttg ggaatagccc tgaccagagt gtgtgacagg atgacaggga
     6781 tgcccagtct cctcaccctg ttggcctgta ccgggaagcc tccctcgtga ggcattgcct
     6841 caatgatggc tttgaccttt gccccggcct gcatcaactg atatgaggtg aggtacccga
     6901 tgttaccggc acccacggta aggatgttcc tgcccagcag ggtgaattcc agattcatca
     6961 tcttctggat cactgcagcg gtataaaccc cgggcagatc atcgttgccg aaggccggca
     7021 tgaagggaat tgcgccggtg gcaacaatca tatactgagc gtcaacgtag aagatctccc
     7081 cggtaagcat gttcttggca gctatcctgt tcccctcaag aatatcccat atggttgagt
     7141 tgagcatgat gcc
//
LOCUS       DAOPBK010000140         7095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9503_length_7095_cov_4.335085, whole genome shotgun sequence.
ACCESSION   DAOPBK010000140 DAOPBK010000000
VERSION     DAOPBK010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9503_length_7095_cov_4.335085"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1995)
                     /locus_tag="PLA17_08485"
     CDS             complement(190..1995)
                     /locus_tag="PLA17_08485"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HPK85492.1"
                     /translation="MMKRIFTLLFFLTAVIALNAQEGKRLTILHTNDFHSHLQGFGPE
                     SAYTPGVADNDPTIGGMARIAGIIDEVRRQNPGSTLVVDAGDCIMGTLFQALEPETGF
                     QHALMKKAGYDVVAMGNHDFDFGPEAFAGILRKATRRGEIPIMLMGNAVTDRNDPADD
                     AFEALISDGLVKPFTVREINGIRTGIFSLIGKDARESAPYAHPVTFEKIIPASRKLVR
                     SLKKEGCDLIICLSHSGIRKDKKGNWSGEDVKLATKVKGIDLIISGHTHTLLMEPLVV
                     NGVPIMAVGDNGRFVGRIDLMALNGSIKLEKYEIIPVDDRATAPDEIQSEIVLQQQKI
                     NETILNSLGMTYTMPVAVAPYTVSYDEYGKAADSNLGALVADAIYDYVNHEGPGTDIA
                     IVAAGVLRDPIQPGVQSVADIFRVMPLGSGNDNVPGYSLSKMWITGKELKNITEILLF
                     TSATASSNYPYYSHLRIEYDPEGGLFNKVRKIEITDKEGNVQEVNTDKNDPKLYSIVA
                     NSYMADNLGLVKKKTFGLIKVEPKDSNGNIVARMDDFIMDFNDVQPGIQEGKEWIALL
                     KYLQRFKPAEEGGLPVIPDYYLNPENSLVPVSRAK"
     gene            2325..3287
                     /locus_tag="PLA17_08490"
     CDS             2325..3287
                     /locus_tag="PLA17_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK85493.1"
                     /translation="MIREIKAHDLDTEKFIAEKVSEIRAAVGEGTAINALSGGVDSSV
                     VTMIGHLALGDRLRTVLVENGLMREGEPEAVVSIFEKLGVKVEIIDARQEFFEALKGI
                     TDPEQKREAITQTFYKKVFGRIVRESGAKYLLQGTILTDIDETVAGIKRQHNVFAQLG
                     IDPQETFGYHILEPLIQLRKEGVRMTGRATGLPEEMFNRIPFPGPALAARIIGEVTQE
                     KLDIVRKATVIVEKLLAGSGAFQYMAILHDDRVTGMRDRKRDFGRQIEVRCWDSIDAR
                     TATPTRLPFELLEQLASEILESVPGVVSVTYNIATKPPSTIEAV"
     gene            complement(3268..4581)
                     /locus_tag="PLA17_08495"
     CDS             complement(3268..4581)
                     /locus_tag="PLA17_08495"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPK85494.1"
                     /translation="MTYRETIEYLFSLLPAYHRVGKPAYKGNLDNTLELDRYFGHPHS
                     RFRTVHVAGTNGKGSVSHMTASVLQEAGYRTGLYTSPHLKDFRERIKIDGEMIPEEEV
                     VRFVADHDGIISRIKPSFFELTVALAFDYFARMKVEIAVIEVGMGGRLDSTNIITPLL
                     SVITNIGHDHMEFLGRTLGAVAVEKAGIIKEGVPVVIGETQPETRDVFQQRAEAMHAP
                     VTFADNRFRCSPGRISPDSSLRRFTLSGFDGGMTLKGETPLGGLAQRKNLQTTAAAVE
                     RLSADLGLSHEHFLRGVAGVVQNTGLMGRWQILSRSPLTVCDTGHNREGLQYVMRQVR
                     ATPKEKLHMVIGFVDDKELGSVLPLFPHDALYYFTRASVPRALDENLLKAEAGKYGLT
                     GEAFRSVPLAVEAARTAAGQRDMIFIGGSTFVVADCLPDLKPPLL"
     gene            4709..6046
                     /locus_tag="PLA17_08500"
     CDS             4709..6046
                     /locus_tag="PLA17_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPK85495.1"
                     /translation="MAKKKKQKKIFILDTNVLLHDFTCIYNFEENDVIIPIVVLEELD
                     KFKRGNDIINFHAREFTRELDKIAGDMLLTGNIPLGQNLGTLHIETGKQFSERVSESF
                     PERTPDHRILAIADFITQENKDKVVVLITKDINLRMKAKSLGIRSEDYQNDKVANLDD
                     LYKGIRIIENIDHDLINKLYENADGVPASELKIKDTEPGHHYFILRNSNSSALAHYDP
                     ATKTLERVLKQTTYGIEPRNAEQTFALDALCDPDIQLISLTGKAGTGKTLLALAAALH
                     QHKRYKQIFLARPIVPLANRDMGFLPGDVKEKMEPYMQPLFDNLTVIKHRFSAQSPEF
                     IRINDMIKDDKLVITPLAYIRGRSLSNIFFIVDEAQNLTPHEVKTIITRAGEGTKMVF
                     TGDIEQIDSPYLDSGSNGLSYLSDKMKGLDLFAHVHLVKGERSFLAELASKLL"
     gene            6094..>7095
                     /gene="mtaB"
                     /locus_tag="PLA17_08505"
     CDS             6094..>7095
                     /gene="mtaB"
                     /locus_tag="PLA17_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPK85496.1"
                     /translation="MKRKVAFHTLGCKLNFAETSTIARTLPDDRFERVSADSRADIYV
                     INTCTVTDTADRKCRQAVRKFAHRNPDAFIAVVGCYAQMRREEAASIEGVDLVLGTYE
                     KFDLASYIDNLQKRTAPGIHSCETVDTDEFMASWSAGERTRSFLKVQDGCDYHCSYCT
                     VPLARGKSRNPAISEIVAEAEKIVAGGVMEIVLTGVNVGDFGKSTGETLEQLLESLVK
                     VNGLQRLRLSSIEPNLVTDRIIDIVSEGKVLLPHFHIPLQSGNDRVLGLMRRRYRREV
                     FRDRVMKIRERIPDAGLGADVIVGFPGETEEEYADTYSFLESLPLSYLHVFAFSPRPG
                     "
BASE COUNT         1806 a         1861 c         1718 g         1710 t
ORIGIN      
        1 agatacaaat cacacaagga tctggtaaca attctgaaga aaggcacccc tgacgagttc
       61 cgtgaaggaa tgagaagaca cctggaaaac cactttgcca ggatactggc aagccacaga
      121 actcaggcag agtgatgtaa aaagtataat cagccagccg gaggctccgg caatctcccg
      181 catttataat tacttcgctc tgcttactgg aaccagtgaa ttttccggat taaggtaata
      241 atcgggaatc acgggcaatc caccctcttc tgccggtttg aatcgctgaa ggtatttcag
      301 gagcgcaatc cattccttgc cctcctgtat gccaggctga acatcattga agtccataat
      361 gaaatcatcc atcctggcaa ctatgtttcc gttgctgtct tttggctcaa ccttgatcaa
      421 accaaatgtc ttcttcttca caaggccaag gttgtctgcc atatacgaat tggccactat
      481 cgaatagagt ttcggatcat tcttgtcggt atttacctcc tgcacatttc cctctttatc
      541 cgtgatttca atcttgcgca ctttgttgaa cagtcctcct tccgggtcat attctatgcg
      601 tagatgtgaa taatagggat aatttgacga ggctgtggct gaggtaaata gaagtatctc
      661 ggttatgttc tttagctcct tccccgtaat ccacattttt gacagcgagt atcctggcac
      721 attatcattc ccggagccga gtggcattac cctgaagata tccgccacgc tctgcactcc
      781 aggttgtatc ggatcacgga ggacgcccgc agccacaatg gcgatgtcag tgccggggcc
      841 ttcatgattc acatagtcgt atatggcatc tgccacaagg gcgccaaggt tactgtcagc
      901 tgcctttccg tattcatcat agcttactgt gtatggtgca acggcaaccg gcatggtgta
      961 ggtcatcccc agggaattca gtattgtttc gttgatcttc tgctgctgca atacaatttc
     1021 tgattgtatc tcatccgggg cagtggctct gtcgtcaaca ggaatgattt cgtatttttc
     1081 gagttttatg cttccgttga gtgccatcaa atcaattctg ccgacgaacc ttccgttgtc
     1141 tccaacagcc atgatcggca caccgttaac aaccagaggc tccataagga gcgtatgggt
     1201 gtgaccgctg ataataaggt caatcccttt cacttttgtt gccagtttca catcctcacc
     1261 actccagttt cctttcttat ctttgcgtat gccgctgtgt gacaggcata taataagatc
     1321 gcaaccctct tttttcaggc ttctgaccag ttttcttgat gccggaatga tcttttcaaa
     1381 ggttaccggg tgtgcatagg gagctgattc tcttgcatcc tttccgatga gtgaaaaaat
     1441 acctgtcctt atgccgttta tctcacgtac agtaaatggt ttaacgagac catcactaat
     1501 caatgcctca aatgcatcgt cagcagggtc attgcgatca gtgacggcat ttcccatcag
     1561 cataatgggt atctccccgc gccgggtcgc ttttctcagg atgccggcaa aggcctccgg
     1621 cccgaagtca aaatcatgat tacccatggc aaccacatca taccccgctt ttttcatcag
     1681 cgcatgctgg aatccggtct ccggctccag cgcctggaag agtgtcccca taatacagtc
     1741 gcccgcatcc accaccagcg ttgatccggg attctgcctg cgcacctcat caataatacc
     1801 ggcaatgcgt gccattcctc cgatagtggg atcattgtca gccacgccgg gcgtataggc
     1861 agactccggg ccaaagccct gaagatgcga atggaaatca ttggtatgaa gaattgtcag
     1921 cctcttcccc tcctgggcat tgagtgcaat aaccgctgtg aggaaaaaca acagggtaaa
     1981 aattcttttc atcataatat ggttttgatt ttaaaggtat aattaaaccc acatgactga
     2041 aaatcatata gatactcgtt tacgtatttg acacatgcgg gattcatcat atatgaaatt
     2101 tgggatcaag ttaacaaaaa gcttcagatc actgcaacag aggggctaaa agaattactc
     2161 tgttctgtct gaggtcattg tttttcagat ttacgccatg gtaaagacag tgtcaggttt
     2221 ttccctgcga tggttgaaag agtgctcatt tcttcccggt caattatagc tcatggcaat
     2281 ctgccttgat tatctttgca tcaatcaaaa aaccagcagg agaaatgatc agggaaataa
     2341 aggcgcatga tcttgacacc gaaaagttca ttgccgaaaa ggtcagcgaa atcagggccg
     2401 ctgtgggcga aggcacagca atcaacgctc tctcaggagg agtagactca tcagttgtca
     2461 ctatgatagg ccatctggcc ctgggcgaca ggctcaggac agtgttggtc gagaacggtc
     2521 ttatgcggga gggagaaccc gaagccgttg tttcaatatt cgagaagctt ggtgtgaagg
     2581 ttgagattat cgacgcccgg caggagttct tcgaagccct gaagggtata acagatcccg
     2641 agcagaaacg cgaagccatc acacagacat tctataagaa ggtatttggc cggatcgtca
     2701 gggagagcgg cgcaaaatat ctgctgcagg gaaccatact gacagatata gacgagactg
     2761 tggcaggcat caaacgtcag cacaatgtct tcgcccagct gggcattgac ccgcaggaga
     2821 ctttcggata tcatatcctt gagccactga tacaactgcg caaggagggc gtcaggatga
     2881 ccggccgtgc taccgggctt cccgaagaga tgttcaaccg gatacccttc ccgggtcctg
     2941 ccctggcagc ccgcattatc ggtgaggtga ctcaggagaa gcttgatatt gtaagaaaag
     3001 ctacggtaat agtggaaaaa ctgcttgccg gcagcggagc tttccagtat atggccatcc
     3061 tgcatgacga ccgggttaca gggatgcgcg accgcaagcg cgatttcggg aggcagatag
     3121 aggtgcgctg ctgggacagc attgatgccc gtaccgctac ccccaccagg ctgccgtttg
     3181 agctgcttga gcaacttgca tccgaaatcc tggaaagcgt cccgggagta gtctccgtta
     3241 cctataatat agccactaaa ccaccctcta caatagaggc ggtttgagat caggcaggca
     3301 gtcagcaacc acaaatgttg atccgcctat aaagatcata tcacgctggc cggcagcggt
     3361 acgggcagcc tcgacagcca aaggcacgga gcggaaggct tcacctgtca ggccgtactt
     3421 cccggcttca gcttttagca gattctcatc cagcgccctg ggcacggaag ccctggtaaa
     3481 ataatacaag gcatcatgcg gaaacagagg cagaaccgat cccagctcct tgtcatcaac
     3541 aaaaccgatg accatatgaa gcttctcctt cggcgtcgcc ctgacctgcc tcatcacata
     3601 ctgcagacct tccctgttat gaccggtgtc gcatactgtc agtggtgacc gcgacaggat
     3661 ctgccacctg cccatcagcc cggtgttttg aaccacacct gccacacccc tgaggaagtg
     3721 ttcgtggctg agcccgaggt ctgctgagag tctctccaca gccgctgccg tggtctgcag
     3781 gttcttcctt tgcgccagtc ctccaagcgg tgtttcaccc ttcagggtca taccgccatc
     3841 aaagcctgaa agagtgaacc gcctgaggga gctgtccggg gaaatccttc ccggactgca
     3901 cctgaaccgg ttatcggcaa aggtgacagg cgcatgcatg gcttcagcgc gctgttgaaa
     3961 aacatcccgt gtctcaggct gagtctcgcc tatcacaaca ggcacaccct ccttgatgat
     4021 cccggccttc tccacggcaa ctgcccccag ggtacgcccc aggaactcca tgtggtcatg
     4081 ccctatattg gttatgactg acagcagggg tgtgataata ttggtcgaat caagcctgcc
     4141 tcccatccca acctcaatga cagcaatctc aaccttcatc ctggcgaagt agtcaaacgc
     4201 cagcgcaacg gtcagctcaa agaatgatgg ctttatcctg cttatgattc catcatggtc
     4261 tgcaacaaac ctgaccacct cctcctccgg aatcatctca ccgtcaatct ttatcctctc
     4321 cctgaaatct ttcaggtggg gagatgtgta gagacctgtc ctgtatccgg cctcctgcag
     4381 caccgaggca gtcatatgcg atactgatcc cttcccgttg gtacctgcaa cgtgtaccgt
     4441 acggaacctt gagtgcggat gaccaaaata gcggtcaagc tcaagcgtat tgtccaggtt
     4501 acccttgtat gcgggcttgc ccacacggtg gtatgcaggc aacaggctga agagatattc
     4561 aatggtttcc cggtatgtca tgttgataac ctttgtgcgg ttgttcgcaa atttataaaa
     4621 agcgtttttc acacgcatat ggcaccgtta tctttgtttc tgtggtacta tctgcgccgg
     4681 agatgctgcc atttaaacca aatctgttat ggcgaaaaag aaaaagcaaa agaagatttt
     4741 tattcttgac acaaacgttc tgctgcatga cttcacatgc atctacaatt ttgaggaaaa
     4801 tgatgttatt attcccatcg tggtgctgga agagcttgac aagttcaagc ggggcaatga
     4861 cattataaat ttccatgccc gggaatttac gcgtgagctt gacaaaatag ccggtgacat
     4921 gctcctgacg ggcaatattc cccttggcca aaacctgggc acactgcaca ttgagaccgg
     4981 gaagcagttt tcagagaggg tctccgaatc attcccggaa agaacccctg atcaccggat
     5041 actggcaata gccgatttta ttacccagga aaacaaagac aaagtcgttg tcctgataac
     5101 aaaggacatc aatctcagga tgaaggcaaa gtcactgggt atcaggtctg aagactacca
     5161 gaatgacaag gttgccaatc ttgatgatct ctacaagggg atcaggataa tcgagaacat
     5221 tgatcatgac ctgatcaaca agctttacga gaatgctgac ggggttcctg cctcggagct
     5281 caaaatcaag gatacggagc ccggacatca ttattttatc ctgcgaaaca gcaattccag
     5341 cgcccttgcc cattatgatc ctgccacaaa gacgctggag agggttctca agcagaccac
     5401 ctatggcatt gaaccccgga atgctgagca gacctttgcc ctcgacgcac tatgtgaccc
     5461 tgacatacag ctcatctccc tgaccggcaa ggccggtaca ggcaagaccc tcctggcact
     5521 tgcagctgca ctgcaccagc acaagcgcta taagcagatc tttcttgcaa ggcctatagt
     5581 tccgctggcc aaccgcgata tgggcttcct gcctggtgac gtaaaggaaa agatggagcc
     5641 ctacatgcaa ccgctctttg acaacctgac ggtcatcaag cacagattca gtgcccagag
     5701 cccggagttc atccgcataa atgacatgat aaaggatgat aagctggtga tcacccctct
     5761 ggcatatatc agaggccgca gcctctcgaa catcttcttc atcgttgatg aagcccagaa
     5821 ccttactccc catgaggtga agacaattat caccagggcc ggcgagggaa caaaaatggt
     5881 cttcacgggt gacatcgagc agattgactc gccctacctt gactccgggt caaacgggct
     5941 aagctacctc tctgacaaga tgaagggact ggatctcttc gcccacgttc acctcgtcaa
     6001 aggcgagaga agcttcctgg ctgaactggc aagcaaactg ctctgatccg gcttttttgc
     6061 tatttttgca tcaggaacag caaccattta attttgaaga ggaaagtagc gtttcacaca
     6121 ctgggatgca agcttaattt tgccgaaact tccacaattg cacgcaccct gcccgatgac
     6181 aggttcgaga gggtatctgc tgacagtcgt gctgacatat atgtaatcaa cacatgtacc
     6241 gtcactgata ctgctgaccg caaatgcagg caggctgtaa ggaagttcgc ccaccgcaat
     6301 cctgacgctt tcattgccgt cgttggttgc tatgctcaga tgagacgcga ggaggcagcc
     6361 tccatagaag gagttgatct ggtcctcgga acatacgaga aatttgacct cgcctcctat
     6421 atcgacaatc tccagaaacg aacagctccc gggatccact cctgtgaaac cgttgacacc
     6481 gacgaattca tggcttcatg gtcggccgga gaacgaacac ggtcattcct caaggtacag
     6541 gacggatgtg actatcactg ctcatactgc accgttcccc ttgccagggg gaagagcaga
     6601 aatcctgcca tcagtgaaat agtggccgag gcagaaaaaa ttgtcgccgg aggagtcatg
     6661 gagatagtcc tcaccggagt caatgtgggc gacttcggga agagcaccgg agagaccctc
     6721 gaacaactcc tggaaagtct ggtaaaggtg aacggactgc aaagattgcg actgtcatct
     6781 attgagccga acctggtcac tgaccggatc attgatattg tctctgaagg aaaggtgctg
     6841 ctgcctcact tccatatacc tcttcagagt ggaaatgaca gggtgctcgg gcttatgcgc
     6901 cggcggtacc gcagggaggt gttccgcgac agggtgatga agatcaggga aagaattccc
     6961 gatgcgggcc ttggggcaga tgtcatcgtc ggcttcccgg gcgagacgga agaagagtat
     7021 gcagacacat acagcttcct tgagagcctc cccctctcct atctccatgt ctttgccttt
     7081 tcaccacggc cgggg
//
LOCUS       DAOPBK010000141         6963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9702_length_6963_cov_4.323104, whole genome shotgun sequence.
ACCESSION   DAOPBK010000141 DAOPBK010000000
VERSION     DAOPBK010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9702_length_6963_cov_4.323104"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PLA17_08510"
     CDS             <1..884
                     /locus_tag="PLA17_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85497.1"
                     /translation="KNGKLQLLCAGRDYMHLIDRNGNYVDKYPVKMQSPASNTLALFD
                     YENNKEYRLFLAGEDRKIYAYDRSGTPVRGWNLFTTRGKVTDPVSFFRVRGKDYLFVA
                     DDQAVYVLDRTGNIRVAHQEPLLKAPGSAARLAVEDDPAIIFSAPDGSTVRLLFDGTV
                     NKESITGISASHRSEFGDLDGDDVTDRLTIDQGVLIAFDGSGNEMWRYSAGGEELAGP
                     WFVQTGTDEMRIAIYDTGKRMLHLIRRNGASVSGFPRPAGPLFNTGKVTNKSTWNLIS
                     VEDENYLCNHTLNSASK"
     gene            1028..2482
                     /locus_tag="PLA17_08515"
     CDS             1028..2482
                     /locus_tag="PLA17_08515"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPK85498.1"
                     /translation="MKRSILALILLLSLVTTGHSQNSQVLYFMNLPQATTLNPAFQPA
                     GRIYIGLPGISDISVRLDNNFLSLSDFFINGVISDSTLSFLEKGPWVNNFLDGLGKNN
                     SLEPQVGVKLLGFAFTVKDNLRFSLDITERAEVNMVFPGDFFRLGLGGNSDFIGKTLD
                     FSSLRTDMMVYHETGLGASANITEKLRIGGRLKILSGVASAYLNNNHFTLTVNEDNTH
                     TAVADFSMNISAPVFFTTDSDGSVHGAEFNNARFNDAWNVISYLKEMANPGLGIDLGA
                     EYRFSDKLVVSAAVNDLGFIRWKRDLSQLTVNSTFQFNGQTMQDVYDESVEFGEMMNW
                     AIDSLQNVLRLEDLPQKYTTYLPAKLTAAFSYTPVRYFTAGLLSQTRFEGPQVHQSVT
                     LSGNFHFGNVFSTTLAYTAANRRYDNIGFGMAVRGGPSQFFVVVDNICLKFTSYTSDE
                     GRIMIPAKINTVNVRLGFNLLFGYRERVKALPPM"
     gene            complement(2491..>2817)
                     /locus_tag="PLA17_08520"
     CDS             complement(2491..>2817)
                     /locus_tag="PLA17_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK85499.1"
                     /translation="KRVAIARAIVLKPKFLFCDEPNSGLDPKTSIVIDELIREITVEL
                     NMTTIINTHDMNSVMENGEKIIFIEDGEKCWEGNKNEILKSDCSKLNDFVFANSLARQ
                     LKDSSV"
     assembly_gap    2818..2917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2918..3401)
                     /locus_tag="PLA17_08525"
     CDS             complement(<2918..3401)
                     /locus_tag="PLA17_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK85500.1"
                     /translation="MIRAENISKSFAGRRVLTDISAIFEPGKTNLIIGKSGSGKTVFL
                     KCLVGLLDVDEGEIYYDDVAFTRLDFRGKKELRQQMGMLFQGSALFDSMSVEENVRFP
                     LDMFTSMSYQEKLDRVNFCLDRVNIVDSNRLFPSELSGGMKKRVAIARAIVLKPKFLF
                     C"
     gene            complement(3398..4141)
                     /locus_tag="PLA17_08530"
     CDS             complement(3398..4141)
                     /locus_tag="PLA17_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK85501.1"
                     /translation="MFRFLSGFGSYWILLGRVFARPEKHSIYYRQTMKELVNLGLRSI
                     GIVTIISFFMGAVITLQTAYNTENPIYPTYLIGLGARDSILLEFSSTIVALILAGKVG
                     SNIASEIGTMRVTEQIDALELMGVNSASYIILPKVVATVVFNPFLTLISMITGITGGW
                     VVGTAAGVITSQNYIYGIQYAFIPYYITYALIKTVVFAFIISTVSAYQGYYVEGGSLE
                     VGRASTKAVVYSSVTLLLFNVILTQLLLS"
     gene            4256..5341
                     /locus_tag="PLA17_08535"
     CDS             4256..5341
                     /locus_tag="PLA17_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPK85502.1"
                     /translation="MKNRYVLIMAGGVGSRFWPLSRREKSKQFLDILGIGETLIQMTY
                     RRFTDICPPENIYVVTNEDSSDTVVSQLGIDPDHVLAEPLRRNTAPCIAYGVFRIMAE
                     NPDAVIAVAPSDHLIRKQDKFRMVMDEAFRFASGNDVLLTLGIKPDRPETGYGYIQAN
                     TMQPVAGYEGLHKVKAFTEKPELAMAKLFLESGDFYWNSGIFIWKASTILESYSRYLP
                     DIFHTFNEGKELFGTAGEKEFITQAYSQCSSISVDYGIMEKADNVYVICTDLGWSDLG
                     TWSSLYLQSEHDRNDNAVLNSKALTYNSEGNIISLPQGKIAVIQGLDDYIIIDSGDVL
                     LIVSRDQEQNIKTYLNDVGREAGENYL"
     gene            complement(5503..6357)
                     /gene="gldN"
                     /locus_tag="PLA17_08540"
     CDS             complement(5503..6357)
                     /gene="gldN"
                     /locus_tag="PLA17_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HPK85503.1"
                     /translation="MKRTILAGLAGLLMCTGTMAQSFGDIYTRQMPDNQKVNYTYLNE
                     SDVIWSKRLYRIIDLREKMNLNLYYPSLKYDADENRFKSEMSDGRMNFSGILLNEILS
                     GRVPATDGDAMTVPTTYNDIAAKMNRDTLTIAVVGADGLEHDSTVTNMENPSDIKQLM
                     LLEEWFFDKKHSRLDVRIIGICPIYMGYDPITSRLVKRRLFWIRYEDVRQALAASEAF
                     NSFNDAQRISFEDLMLQRRFSGYIVAESNVYDDRSINQYKMGSSQIFEAERIKNEIFN
                     FEQDLWEY"
     gene            complement(6376..>6963)
                     /locus_tag="PLA17_08545"
     CDS             complement(6376..>6963)
                     /locus_tag="PLA17_08545"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HPK85504.1"
                     /translation="NVVVSPTAMNVLYQGIQNPLDISVPGIGSDKLNVRMTNGEIKKG
                     KYRNYRGEYVAQPRTVGQNAQIIVSANVDGKVQTFPPVEFRVRRLPDPEARFANLKEG
                     NVLRSVAAAQQVVTAVLENFEFDLTYTVTGFTVSVNDKGYEITAESNNNRLTDKQKSL
                     ISNLRAGQKLIIEKIRAVGPDGRTRDLNPIILKIN"
BASE COUNT         1723 a         1605 c         1757 g         1778 t
ORIGIN      
        1 acaagaatgg caagctgcag cttctttgtg ccggccgtga ctatatgcac cttatagacc
       61 ggaacggcaa ctatgttgac aaatacccgg taaagatgca gtcaccggct tcaaacaccc
      121 ttgccctctt tgattatgag aacaacaagg agtacaggtt gttcctggcc ggggaagacc
      181 gtaagatata tgcctatgac cgttcaggca caccggtcag gggatggaac ctcttcacca
      241 ccagggggaa ggtcaccgat cccgtatctt tcttcagggt caggggcaag gattacctgt
      301 tcgtggcaga tgaccaggct gtttatgtgc ttgaccgtac cggcaatatc agggtagccc
      361 atcaggagcc gttgctgaag gctccgggtt cggccgccag gctggcagtt gaagatgacc
      421 ctgccattat cttttccgct cctgacggtt caactgtcag gctgctgttt gacggcacgg
      481 taaacaaaga gagcatcaca ggaatctccg cctcacacag gtctgagttc ggtgatcttg
      541 acggcgacga tgtgactgac cgtcttacca ttgaccaggg tgttctgata gcctttgacg
      601 gttccggcaa tgagatgtgg agatacagtg ccgggggaga ggagctggca ggaccgtggt
      661 ttgtacagac cggaacggat gagatgagaa ttgccatata tgataccggg aaacggatgc
      721 ttcatctcat cagacgaaac ggagcttcag tgagtgggtt cccgcgtccg gcaggtcccc
      781 tgttcaacac aggaaaggtg acaaataaga gtacctggaa cctcatctca gtagaggatg
      841 agaactacct ttgtaatcat acactgaatt ccgcatcaaa ataatgacag attgctaacg
      901 ttttatttac agacgttata atcaattaat tagtgcgccc attttaagat agatgataaa
      961 tcttgttatt caggcagtta aaagcaatca ttttaaaata tactgaacta ttgaacaata
     1021 aaaccgtatg aaaagaagta ttctggcact gatcctgctg ctgtcactgg tcaccaccgg
     1081 ccattcacag aacagccagg ttctttattt catgaacctg cctcaggcaa ctaccctcaa
     1141 tccggccttt caaccagcgg gcaggattta cattggacta cccggcatat cagatatctc
     1201 cgtcagacta gacaacaact tcctgagtct gtcagacttc tttataaatg gtgtcatctc
     1261 tgactctacc ctctctttcc ttgaaaaggg accatgggtg aataacttcc tggatggtct
     1321 gggcaaaaat aactcgctgg agccgcaggt cggggtcaaa ctgctcggtt tcgcctttac
     1381 ggtgaaggac aacctgaggt tcagccttga catcactgaa cgggcagagg tcaacatggt
     1441 atttcccggt gattttttca ggctgggcct tggcggcaac agtgacttca tcggtaaaac
     1501 tctggacttt tcttcgttaa ggacagacat gatggtgtac catgaaacag gcctgggggc
     1561 ttcagcaaac atcaccgaaa agctgaggat aggaggcagg ctgaaaattt tgtcgggagt
     1621 ggcatctgca taccttaaca acaaccattt cacactcaca gttaatgaag ataacactca
     1681 tacagctgtt gccgatttct caatgaacat cagtgccccg gtattcttca caacagatag
     1741 tgacggctct gttcacggag cagaattcaa caatgcaagg tttaatgatg cctggaacgt
     1801 gatatcatac ctgaaggaga tggctaaccc gggtttggga attgacctgg gtgcggaata
     1861 caggttcagt gacaaactgg tagtgtcggc agccgtgaat gacctgggtt ttatcaggtg
     1921 gaagagagac ctgtcacagc ttacggttaa ttcgaccttt caattcaacg ggcagacaat
     1981 gcaggatgta tatgatgaga gcgtcgaatt tggcgagatg atgaactggg ccattgactc
     2041 tctccagaat gtactgaggc ttgaagattt gccgcagaag tacactacat acctgccggc
     2101 aaaattaacc gcagccttca gctacacacc ggtcaggtac tttacggcag gattgctgtc
     2161 gcagacacgg tttgaggggc cgcaggtaca ccagtcagtg acgctctcag gtaactttca
     2221 ttttggcaat gtcttttcaa ccacattggc atacactgca gccaacaggc gctatgacaa
     2281 cattggcttc gggatggcgg tgaggggagg gccttcacag ttctttgtcg tggttgacaa
     2341 tatatgctta aagtttacca gctatacaag cgatgagggg agaatcatga ttcctgcaaa
     2401 aatcaatact gtaaatgtac gactgggttt caacctgttg ttcgggtaca gggaaagggt
     2461 aaaggctctt cccccgatgt agggagtttt ctatactgaa gaatccttga gctgtcttgc
     2521 cagtgagttg gcaaagacga agtcattgag tttgctgcag tctgacttca ggatctcgtt
     2581 cttgttgcct tcccaacatt tctctccatc ttcaatgaat atgatctttt ctccgttctc
     2641 catcacggag ttcatgtcat gagtattgat aatggttgtc atgttgagct cgacggtgat
     2701 ctcccgtatc agttcgtcaa tgactattga tgtcttcggg tcaaggcctg agttgggctc
     2761 gtcacagaag aggaacttcg gtttgagtac aatggcacgg gctatggcca cccgtttnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac agaagaggaa cttcggtttg
     2941 agtacaatgg cacgggctat ggccacccgt ttcttcatcc ctccggatag ctcggaagga
     3001 aaaagccggt tggagtctac gatattcacc cggtcaaggc aaaagttaac cctgtcaagt
     3061 ttttcctggt atgacatgct ggtgaacatg tcaagcggaa acctgacatt ctcctcaaca
     3121 ctcattgagt caaagagggc gcttccctgg aagagcatgc ccatttgctg ccggagctcc
     3181 tttttaccac ggaagtcgag acgggtgaaa gcgacatcat cgtagtagat ttctccctca
     3241 tccacgtcaa gcagtccgac aaggcatttg agaaagacgg ttttcccgga gccgctcttt
     3301 ccgatgatca ggttggtctt gcccggttcg aagatggcgg atatatcagt gagcacccgt
     3361 cggccggcaa atgatttgga aatattttcc gccctgatca tgagagaagc agttgtgtga
     3421 gtatgacatt aaagagcaga agggtcacgc tgctgtagac cacggctttt gtgcttgccc
     3481 ggcccacctc cagtgagcct ccctccacgt agtacccctg gtaagccgat actgtggaga
     3541 taatgaaagc gaagacaact gttttgatca gggcataggt gatgtagtac gggataaagg
     3601 catattgtat gccatagata taattctgag aagtgattac accggcggcg gtgccgacga
     3661 cccatccgcc ggttataccg gtgatcatgc ttataagggt gagaaagggg ttgaagacca
     3721 cagtggccac caccttgggg aggatgatgt aagaggcgga gttgacaccc ataagctcaa
     3781 gggcatcaat ctgttccgtc actctcattg tgccaatctc cgatgctatg ttggagccca
     3841 ccttgcctgc aaggatgagg gcaacgatgg ttgatgagaa ctccagcagt attgagtcac
     3901 gggcccccag gccgatcagg taggtggggt atatggggtt ctcggtattg tatgctgtct
     3961 gaagggtaat aaccgctccc atgaagaaag agatgatggt gacaattcct attgaccgga
     4021 gaccaaggtt aaccagctcc ttcatggtct gacggtaata gatggaatgc ttctcaggtc
     4081 ttgcaaaaac ccttccgagc aaaatccagt aagagccgaa tcctgacaag aatctgaaca
     4141 tgtggaataa ttgtctggtc aaatttaaca ataatttgta gcttctcagc cggctccggt
     4201 attcctgcga tggtttttat atctttgcgg cagtgcagac cgatcaagaa caactatgaa
     4261 aaatcgctac gtactgatca tggccggcgg cgtgggaagc cggttctggc ctttgagcag
     4321 gagggaaaaa tccaagcagt tccttgatat cctcgggata ggagagaccc tgatacagat
     4381 gacttaccgg cgttttacag atatatgtcc gcctgagaac atctacgtgg taacgaatga
     4441 agacagcagt gatactgttg tcagccagct ggggatagac cctgaccatg ttctggctga
     4501 accgctgagg cgcaacacag ctccgtgcat tgcatacggt gttttccgca taatggcgga
     4561 gaatcctgat gccgtgatag cggtagcccc ttcggatcat ctcatacgaa agcaggataa
     4621 gttcaggatg gtgatggatg aagccttcag gtttgcatcc gggaatgacg tcctgcttac
     4681 cctcggcatc aagcctgaca gacctgagac cggttacggc tacatacagg ccaatacaat
     4741 gcagcctgtt gctggctacg agggtcttca caaggtaaaa gccttcaccg agaaaccgga
     4801 actggccatg gcgaaactct tcctggagag cggtgatttc tactggaaca gcggtatatt
     4861 catctggaag gcttcaacaa tactggaatc gtacagcagg tatctgcctg atatcttcca
     4921 tacattcaat gaagggaaag agctgttcgg cacagccggt gaaaaagagt tcataacgca
     4981 ggcctattcg cagtgcagca gtatctcggt tgattatggc attatggaaa aggcagataa
     5041 cgtgtatgtt atatgcactg acctgggctg gtcagacctg ggtacctgga gctcgctcta
     5101 tcttcagtca gagcatgaca ggaatgacaa tgccgtgctg aattcgaaag ccctgaccta
     5161 caattccgag ggcaacataa tcagcctgcc gcagggaaag attgcggtaa tacagggtct
     5221 ggatgactat atcatcattg acagcggtga tgttctcctg attgtatcgc gcgatcagga
     5281 acagaatatc aaaacatacc tgaatgatgt cggccgggag gccggggaaa attacctgta
     5341 atcatttggt atataaatgc attgcagaaa tgtgcctata tgcaggtagc gacgggtctg
     5401 agacctgtcg gtacgtggca tgattcatga ttttacatat ataaacgcac aacggcgggt
     5461 ctgaggcccg ccgttgcatc atttcaaatc ctgatcctaa tcctaatatt cccaaagatc
     5521 ctgttcaaaa ttgaaaatct catttttgat tctttccgct tcgaatatct gtgaagagcc
     5581 catcttgtac tggttgattg acctgtcatc gtagacgttg gattcggcaa cgatgtaccc
     5641 gctgaacctg cgttgcagca tcaggtcctc aaaggagata cgctgggcgt cattgaacga
     5701 gttgaatgcc tcgctggcag ccagtgcctg ccttacatcc tcgtacctta tccagaagag
     5761 acgtctcttg acgagtctgg aggtgatggg gtcatagccc atgtagatgg ggcatatacc
     5821 tattatacgg acatcaaggc gcgaatgctt cttgtcaaag aaccactcct cgagcagcat
     5881 gagctgtttg atgtctgaag ggttctccat attggtcacg gtagagtcat gctccaatcc
     5941 atctgctcca accacagcaa tggtcagggt gtcacggttc atcttggcag cgatgtcatt
     6001 atacgtggta ggtactgtca tggcatcacc atcggtggcc ggaactcttc ccgagaggat
     6061 ctcgttgagg aggatacccg agaagttcat ccttccgtct gacatctcag atttgaaccg
     6121 gttctcatcc gcatcatact taagtgacgg atagtagagg ttcaggttca tcttttccct
     6181 caggtcgatt atcctgtata atctttttga ccatatcacg tctgactcat tcagataggt
     6241 gtaattaacc ttctgattat cgggcatctg cctggtgtag atgtcaccga atgactgggc
     6301 cattgtgcct gtacacatca gcaggcctgc gaggcctgcc agaattgtcc tcttcatggt
     6361 ttgtatcttc tgttgttaat taatcttgag aataatcggg ttgaggtccc tggttctgcc
     6421 atcgggtcca acagccctga tcttttcaat tatgagtttt tgtccggcgc gcaggtttga
     6481 gataaggctc ttctgcttgt cggtaagtct gttgttattt gattcggctg tgatttcata
     6541 gcccttgtca ttgactgaga ctgtaaagcc tgtcaccgta tatgtaaggt cgaactcgaa
     6601 gttttcgagt actgcggtca ccacctgctg tgcagcggcc acgctcctga gtacgttacc
     6661 ttccttcaga ttggcgaacc gtgcctcggg gtcggggagt ctccttaccc tgaactccac
     6721 aggagggaat gtctgcacct tgccgtccac gttggctgag acaataatct gtgcattctg
     6781 ccccactgta cgtggctgtg ctacatattc accgcggtaa ttcctgtatt tacccttttt
     6841 gatctcaccg ttggtcatgc ggacattcag tttatccgat ccgatgcccg ggactgaaat
     6901 gtcaaggggg ttctgtattc cctggtagag gacgttcatg gctgtgggtg acacaaccac
     6961 gtt
//
LOCUS       DAOPBK010000142         6916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9773_length_6916_cov_4.505903, whole genome shotgun sequence.
ACCESSION   DAOPBK010000142 DAOPBK010000000
VERSION     DAOPBK010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9773_length_6916_cov_4.505903"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLA17_08550"
     CDS             complement(<1..425)
                     /locus_tag="PLA17_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK85505.1"
                     /translation="MQSATGKLSFREKLGYSLGDGAANFIFQTMMLLQLSFYTDTFGL
                     SAGAAATLFLIARLWGAVCDPIFGALADRTNTRWGKFRPWILWTAIPFGVLGYLAFRT
                     PNLGGTGKLIYAYITYLLLLTVYSANNNPYSALSGVITG"
     gene            619..1662
                     /locus_tag="PLA17_08555"
     CDS             619..1662
                     /locus_tag="PLA17_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK85506.1"
                     /translation="MKKKGEVTIYDISRALNISASTVSRGLNNSPQVRKELRKKIMNK
                     AREMGYQHNKFASNLRQKRTKTLGVVIPRIDSHFMSTVISGMEKVANNAGYQLLISQS
                     EESATLEEANIQALFNSRVDGLLVSLTYETKTKDAFADILRKDIPLVFFDRVFDCGNC
                     VSVVIDNYRAGYEATQHLIDQGCRKIAHIAGSLNRNVYNDRYRGYRQALTDNSMDFND
                     KMLIITDLGDDLGVSIINHLLNSGTMPDGIFTANDSSAVSLICELKRKGFRVPDDVAI
                     VGFNDDPVSRIVEPNLTTVHYPGRAMGEVAASTMIRLLEGEQFGKINSITLSHELIIR
                     QSSIRQGQPESHE"
     gene            1655..3811
                     /locus_tag="PLA17_08560"
     CDS             1655..3811
                     /locus_tag="PLA17_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HPK85507.1"
                     /translation="MNRTLTTLLIILFLVPLSPARADDGYRLWLGYKPVTDRAALEKY
                     RQRFGNVMITGDSPVMKSARDEFIAGLTELTGLKVREVNSPEGRGIVVAGTFSSSPFV
                     ASLLSDVNPGTTGEEGYIIRGIRQGRKNITVVASDGDRGVLYGLFHLLRLISTESLPD
                     NPDILERPATALRLLNHWDNLDGSVERGYAGGSIWNWHLLPGYISPRYRDYARANASV
                     GINGTVITNVNANALVLTPQYLAKVAALAEVMRPYGIKVYLTARFNAPQETGNLSTSD
                     PLDPGVIRWWRDKAAEIYSLIPDFGGFLVKANSEGQPGPQNYGRSHADGANMLADAVA
                     PYGGVVMWRAFVYDNNVPVDRAKQAYNEFVPLDGKFRDNVLIQVKNGPIDFQPREPFH
                     PLFGAMPSTPLMMEFQITQEYLGCATHLVYLAPLFSEVLESDTYAAGSGSEVSKVIDG
                     TLYKHTLTGIAGVTNIGNERNWTGHLFGQANWYAFGRLAWNPELSPAEIASEWIRMTI
                     THDPDIAGGIEEMMLGSREAAVNYMTPLGLHHIMAEGHHWGPGPWVDRGRPDWTSVYY
                     HRADTAGIGFDRTTSGSDAAGQYFPPLDSMFNDPERCPENLLLWFHHMPWDHVMKSGR
                     SLWDEMCLKYQEGVNRVNEYREFWESLRGRIDDGQFEHVRQMLDIQAYEARWWKDACL
                     LYFQTFSRMPIPDGVEQPQHSLEYYRGLKFHYVPGI"
     gene            3842..4876
                     /locus_tag="PLA17_08565"
     CDS             3842..4876
                     /locus_tag="PLA17_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK85508.1"
                     /translation="MNDRHWDTLLKIVRGEETQEPPVGFIIDSPWLPGWYGTTILEYF
                     SDPVTWFEANLHAMKVFPDVMFFPGFWSEYGMCTEPSAFGTKMVWTENDFPHPLKLNC
                     TTDAIASLAKPCVKTDGLLPFVISRMARYHDKVKACGCHYRFACSRGPLNIASFLFGT
                     TDFMMALALTPDEVQKALTVITDFIIDWLELQHEKFPDIKGVLILDDIVGFLGQSDFE
                     TFVLPHLKRIYATFNPEVGFFHNDAAGLVTASYLKETGINLFNFSFNHSITEMRKLAG
                     DGIILLGNLPPRDILAKGTPADVEAGVISMMREAGDIKGVIWSCGGGMPPYASTENIR
                     TFITAVRNYS"
     gene            4889..6376
                     /locus_tag="PLA17_08570"
     CDS             4889..6376
                     /locus_tag="PLA17_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPK85509.1"
                     /translation="MLLLGIDLGSSSVKVSVIDGGKGRTISAASYPPSEMEISAPLPG
                     WAEQDPEQWWKNFTLALAACLKPLGPKRKSIGAIGISYQMHGLVMVDKERKVLRPSII
                     WCDSRAVETGERAFDRLGKDFCLHHLLNSPGNFTASKLAWVKENEPDIYRQIYKIMLP
                     GDYLAMRLTNEIKTTISGLSEGIFWDFMRSEVSQELLDYYGISHDLLADALPSFSLQG
                     ELTAEVAAELGLTPGIPVSYRAGDQPNNALSLNVMKPGEVATTAGTSGVIYGVTDKTQ
                     FDPMSRINTFVHVNHTKRLTRLGVLLCINGTGILNSWLKKNTGAGLTYPEMNDLASAV
                     EPGSNGLFIFPFGNGAERMFGNRDIGASVHGLNFNIHQQGHLYRAAQEAIAYSFRYGL
                     EIMKETGINPTVIRAGEANMFLSKVFRDILSSLTSTVITLYNTDGSVGAARGAGIGCG
                     FYKTEEEAFHGLEVTGSSEPDHHMALIYDARYNEWLDILENYLYE"
     gene            6387..>6916
                     /locus_tag="PLA17_08575"
     CDS             6387..>6916
                     /locus_tag="PLA17_08575"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134074.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HPK85510.1"
                     /translation="MTKKSKSEFFPGIGKIQYEGRNSKNPLAFKWYDPEAKVGRKKMK
                     DILRFAIAYWHSFCGDGTDTFGAKTRDFPYDGLEGDERIRVKLDMAFEFFEKIGAPFY
                     CFHDVDLVGDGSVFEIEKRLEKWVPIIKARQKETGMKLLWGTANVFGNPRYMNGAATN
                     PDFNVLTNVGVQLKNA"
BASE COUNT         1808 a         1688 c         1816 g         1604 t
ORIGIN      
        1 ttcccggtta ttacaccgct gagtgccgag tagggattat tgtttgccga gtagactgtc
       61 agaaggagca ggtaggttat ataggcatag atcagtttgc cggttccccc caggttgggt
      121 gttctgaagg ccagataccc cagaacgccg aaaggaatag cggtccatag tatccagggc
      181 ctgaatttac cccagcgagt gttggtacgg tcagcaagtg caccgaagat agggtcacag
      241 actgctcccc atagcctggc aatcaggaac agcgtggcag ctgcccccgc cgaaagcccg
      301 aaagtatcag tgtaaaagct gagctgaagc agcatcattg tctggaaaat gaagttggct
      361 gcaccgtcac caagactgta gcccagcttc tccctgaaag atagttttcc tgttgcggat
      421 tgcatggtca attgattgtt agaattatac ttgtataagg ttgatagaat cgcatttgta
      481 catggcaccg ccatttacgg tataaaatgt tattaaaagt atatattatg aattaaatgc
      541 gcaaccgatt gcaaaaaata attcagaaaa tttcaaaaat agcagttttc ttacttattt
      601 tgagaaatta tgaccggaat gaagaaaaaa ggcgaagtta caatctatga tatctccagg
      661 gctttgaata tctctgcttc gacggtctcg cgtggcctta acaacagccc ccaggtaagg
      721 aaggaattac ggaaaaagat aatgaataaa gcccgggaga tgggctatca gcataataag
      781 tttgcatcta acctcagaca gaagcgcaca aagaccctcg gggtagtaat cccaagaatt
      841 gacagtcact tcatgtcaac cgtaatttcc ggcatggaga aggtggctaa taatgcgggt
      901 tatcagctgc ttatcagtca gagcgaggaa tctgccaccc ttgaggaagc caatatacag
      961 gcactgttta acagccgtgt tgacgggctg ctcgtctctc tcacctatga aacaaagaca
     1021 aaggatgcat ttgcggatat actgcgaaag gacatacccc tggtcttttt tgacagggtg
     1081 tttgattgcg gcaattgcgt cagcgtggta attgacaact acagggcagg atatgaggcc
     1141 acacaacacc tgattgatca ggggtgcaga aaaatcgcac atattgcagg aagcctcaac
     1201 aggaatgttt acaatgacag gtaccgcgga taccgccagg cgctcacgga caattcgatg
     1261 gactttaatg acaagatgct cataattact gacctgggag atgatctggg ggtatcaata
     1321 atcaatcatc tgttgaacag cggtaccatg cctgacggca tcttcacagc caatgacagt
     1381 tcggccgtat ctctcatatg tgaactgaag cgaaaaggat tccgggtgcc tgatgacgtt
     1441 gccatagtgg ggttcaatga tgatccggtg tcacgaatag tggaacccaa cctgacaacc
     1501 gtacattacc ccggcagggc catgggcgag gtggccgcat caactatgat aaggcttctt
     1561 gaaggtgaac aattcggaaa gatcaattca ataaccctca gtcatgaact gataatcagg
     1621 cagtcatcga tcaggcaggg tcaaccggaa agccatgaat agaactttaa ccactctgct
     1681 gataatactc tttcttgttc cgttatcgcc ggcacgggct gatgacgggt accggctctg
     1741 gctcgggtat aagcccgtaa ctgacagggc cgccctggag aagtacaggc aaaggttcgg
     1801 gaacgtaatg ataaccggag actcaccggt gatgaagagt gcccgtgatg agttcattgc
     1861 aggcctgacg gagctcaccg gcctgaaagt ccgggaagtg aattcaccgg agggacgggg
     1921 catagtcgtt gccggcacat tcagctcatc acccttcgtc gcctcgcttc tttctgatgt
     1981 caatcccgga actaccgggg aggagggtta cataataaga ggtataaggc aggggcggaa
     2041 aaacattaca gtggtagcct ccgatggtga tcgaggggtg ctgtacggac tctttcatct
     2101 tctgagactc ataagcacgg aatcactgcc tgacaatcct gatatcctag aacgtccggc
     2161 cacggcactg cggcttctca atcactggga caaccttgac ggatcggttg agcgcggtta
     2221 tgccggtggc tcgatctgga actggcatct tctgcctggt tacatatctc cacgctaccg
     2281 tgattatgcc cgtgccaatg cctctgttgg catcaacggt acagtcatca ccaatgtcaa
     2341 tgcaaatgca cttgtactta ctcctcaata ccttgcgaag gtagccgccc tggctgaagt
     2401 gatgaggcct tatggcataa aggtttatct cacagcacgt ttcaatgcac ctcaggagac
     2461 tggcaatctc tccacctctg atccgcttga ccctggcgtc atacgctggt ggagggataa
     2521 ggctgccgag atatactcat tgatccccga cttcggtggt tttcttgtga aggccaactc
     2581 tgaggggcag ccggggccac agaactatgg ccgttcacat gctgacggtg caaacatgct
     2641 tgctgacgcc gttgcaccgt atggcggagt ggttatgtgg agggcatttg tatatgacaa
     2701 caatgtgcca gtcgacaggg caaaacaggc atataacgaa tttgtgcctc ttgacggcaa
     2761 attcagggac aacgtgctga ttcaggtcaa gaacggtcca atagattttc agcccaggga
     2821 acccttccat ccgctgtttg gcgccatgcc atcaaccccc ctgatgatgg agttccagat
     2881 tacccaggag tatctcggct gtgccaccca cctcgtgtac cttgcacccc tcttcagtga
     2941 ggtgctggag agcgacacat acgctgccgg cagcggatca gaggtctcga aggttattga
     3001 cggcacgctc tacaaacata ccctgaccgg tattgccggg gtgacaaaca tcggcaatga
     3061 aaggaactgg accgggcacc tcttcggaca ggccaactgg tatgctttcg gcaggctggc
     3121 ctggaacccg gagctgtcgc ctgcagagat agcatcagag tggatcagga tgacaatcac
     3181 ccatgatccc gacattgccg gaggaataga ggaaatgatg cttggctcgc gcgaggcagc
     3241 agtcaattac atgacacccc tgggcctgca ccacataatg gccgaggggc atcactgggg
     3301 gccaggcccg tgggtagacc ggggacgccc cgactggacg tcagtctatt atcatcgcgc
     3361 cgacactgcc ggaatcggat ttgaccgcac cacctcaggc agtgatgccg cgggccaata
     3421 cttccctccc cttgactcga tgttcaatga tccggagaga tgccccgaaa acctgctgtt
     3481 atggttccac cacatgccat gggatcacgt gatgaaatca ggaaggagtc tctgggacga
     3541 gatgtgcctt aaataccagg aaggtgtgaa cagggttaac gaataccgtg aattctggga
     3601 gtcactgagg ggcaggattg atgacggaca gtttgagcat gtgagacaga tgcttgatat
     3661 tcaggcatat gaggcacgat ggtggaaaga cgcatgtctc ctttattttc agacattttc
     3721 acgtatgccc atccccgacg gggtggaaca gccacagcat agcctggagt attaccgtgg
     3781 attgaagttc cattatgttc cgggcatctg attatcttat aacgaagcat aacctgacct
     3841 tatgaatgac agacactggg ataccctcct taagatagta agaggagaag aaacgcagga
     3901 gccccccgtg ggctttatta tagacagtcc ctggcttccc ggctggtacg gtaccacgat
     3961 actcgagtat ttcagtgacc ctgtaacatg gttcgaggcc aacctccatg ccatgaaagt
     4021 atttcctgac gtaatgtttt ttcccggttt ctggtcagag tatggcatgt gcaccgaacc
     4081 ctctgccttc ggcacgaaaa tggtatggac cgaaaacgat tttccacatc ccttaaaact
     4141 gaattgcacc actgatgcta ttgcttcgct ggcgaagcca tgcgtgaaga ccgacgggct
     4201 tcttccgttt gtcataagca ggatggccag gtaccacgac aaggttaagg catgtggttg
     4261 tcactacagg tttgcctgca gccggggccc gcttaacata gcttcgttcc tgttcggcac
     4321 aactgacttc atgatggcgc tggcgctcac ccccgatgag gtgcagaagg cgcttaccgt
     4381 catcaccgac tttatcattg actggcttga actgcagcat gaaaagttcc cggacattaa
     4441 gggagtattg atcctcgatg acattgtagg ctttttgggt cagtctgatt ttgagacctt
     4501 tgtgctgcct catcttaagc gcatatacgc aacattcaac cctgaagtgg gattcttcca
     4561 caatgatgcg gccggcctcg ttactgcttc gtacctgaag gagacaggca tcaacctctt
     4621 taatttcagc ttcaatcaca gcataactga gatgaggaaa ttggccggtg acggtatcat
     4681 cctgcttggc aacctcccgc cgagagacat tcttgccaag ggtactccgg ctgatgtgga
     4741 ggccggtgtc atcagcatga tgcgcgaggc cggcgacata aagggagtta tctggtcatg
     4801 cggaggagga atgcctcctt atgcttctac agaaaatatc cgtactttta tcaccgcagt
     4861 gagaaattat tcataataca aaatccgtat gctattactt ggtattgatc ttggcagctc
     4921 atcagtaaaa gtctccgtta ttgacggagg gaaaggcaga actatctccg ccgcttctta
     4981 tcctccgtct gagatggaga tttccgctcc tctccccggc tgggctgagc aggatcctga
     5041 acagtggtgg aaaaacttca ccctggcact ggcagcatgc ctgaagccgt tagggccaaa
     5101 acggaagagc ataggcgcaa tcggcatctc ataccagatg cacggactgg tgatggttga
     5161 caaagagagg aaggtactcc gcccttccat aatatggtgt gacagccggg ccgtggagac
     5221 gggtgaaagg gcatttgaca ggctggggaa agacttctgc ctgcaccacc tgctcaactc
     5281 tcccggcaat ttcacggcat caaagctggc ctgggtaaag gagaacgaac cggatatcta
     5341 caggcagata tacaagatca tgcttcccgg cgattatctc gccatgcggc tgacgaatga
     5401 gataaagact accatatcag gcctttctga aggaattttc tgggatttca tgagaagtga
     5461 agtatcacag gagttacttg actattacgg aatctcacat gacctgctgg ctgatgccct
     5521 gccttccttc tccctccagg gagagctgac ggctgaggta gctgccgaac tcgggcttac
     5581 gccaggaata ccggtatcct acagggcagg tgaccagcca aacaatgcat tgtcactcaa
     5641 cgtcatgaaa ccgggagagg tagccacaac tgccggcacc tccggagtaa tttacggtgt
     5701 cactgacaag acccagtttg acccgatgtc acgcataaac accttcgtac atgtaaacca
     5761 tacaaaaagg ctgacgcgcc tgggtgtgct gctctgcatc aacggaacag gcattcttaa
     5821 ctcctggctg aagaagaaca ccggcgccgg gctcacttat cctgaaatga atgatctggc
     5881 ttctgcagtg gaaccaggga gtaacggact attcatcttt cctttcggca atggcgccga
     5941 aaggatgttc gggaacaggg atataggagc ttcggtacac ggcctcaatt tcaatatcca
     6001 ccagcaggga catctttacc gtgccgcaca ggaggcaata gcctactcat tccgctacgg
     6061 tcttgagata atgaaggaga caggcatcaa cccgacggta atcagggccg gggaggcaaa
     6121 catgttcctg agcaaggtgt tcagggatat cctttcaagc ctgacatcaa cagtgatcac
     6181 tttgtacaat actgacggct cagttggggc agcccgcggt gcagggatag gatgcggttt
     6241 ctacaagacg gaggaggagg ccttccacgg ccttgaggtg acaggctcaa gcgaacccga
     6301 tcaccacatg gctttgatct atgacgcgag gtacaatgag tggctcgaca ttcttgaaaa
     6361 ctatctttat gaataattat aaacctatga caaaaaagag taaaagtgaa tttttccccg
     6421 gaatcgggaa gattcagtat gagggaagga actcaaagaa tcccctggca ttcaaatggt
     6481 atgaccccga agcaaaggta ggcagaaaga agatgaagga tatccttcgc tttgcgatag
     6541 cctactggca ttccttctgc ggcgacggca ctgacacctt cggtgcgaag acaagggatt
     6601 tcccttatga cggtctggag ggtgatgaaa ggatcagggt caaactggac atggctttcg
     6661 aattctttga aaaaatagga gcaccctttt attgtttcca tgatgttgac ctcgttggtg
     6721 acggatcagt cttcgagatt gaaaagcgcc tcgaaaagtg ggtgccgatc ataaaggcca
     6781 ggcagaagga gacaggaatg aaactgctgt ggggtacggc aaacgtcttc gggaatcccc
     6841 gttacatgaa cggcgctgcc accaatcctg atttcaatgt actcaccaac gtcggtgtgc
     6901 agcttaagaa tgccat
//
LOCUS       DAOPBK010000143         6804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_9959_length_6804_cov_5.577863, whole genome shotgun sequence.
ACCESSION   DAOPBK010000143 DAOPBK010000000
VERSION     DAOPBK010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9959_length_6804_cov_5.577863"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="PLA17_08580"
     CDS             <1..1706
                     /locus_tag="PLA17_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK85511.1"
                     /translation="KGFDKVLILDMMGQLVFYGNPLEAIVHFKTLESHINCGVAECAS
                     CGTVNPETLFNILETKVVDEYGNYTDRRKVSPAEWAEAYRKTLPESPPEEETAAPWSG
                     LQKPGWFRQTFIYLLRDLKSKIANRQYLLLTLLEGPVLGLILSYIIRYIADPSSDIYL
                     FRENENIPIYIFMGIIVALFLGLTISAEEIFHDRKLLRREHFLQLNRSSYLVSKILIL
                     AFISALQTFMFLAVANTILGIRGLFLYYWIALFTTALVANMIGLNISSALNSAIAIYI
                     VIPLLMIPMMVLSGAMFPFDKLNRSLARADRVPVIADLMPTRWTYEALMVKQFTGNEY
                     GRRVYPLKQQLSVSDFNTIYRIPRVQEALATLTAVQGSASITAEQAKEMRLLRNETAT
                     IGAAGIIKPFSGTDSITPDLFTPQLAARLTSWLASADREYRRLSNLADMNLDNYISSN
                     KMALDLLYNNYHNDKLEDIVRKVYEKNKMLEFNDRLIQNVDLIYFEPAEAGPLNFRTH
                     FMAPVKRFMGLRVDTFRFNILLVFSMIIVLYVTLYTELLKRIIMSFETSGRKKRNLNR
                     N"
     gene            1742..2662
                     /locus_tag="PLA17_08585"
     CDS             1742..2662
                     /locus_tag="PLA17_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85512.1"
                     /translation="MTGKIIKIATVILLMSFCSCRNSSVRQGGLTFPVADSVPAAEAE
                     KLSEEAVADIVQNIASPVEIAAILQSMQIPFSAGYLASTQEAEKLTTNFRKAVMLGIY
                     GADLGYLNIYGKTGNSVDVLSAIKRLADGLRVGQFFDFETLKRLSASKSNLDSLLFLS
                     VNSYNQIDNYLRDNDRGSISALMITGVWIEGQYLATQVALSHDDEILRDRIGEQKIIL
                     GDLLMLLRPYRASSEEYGSLYDMMEQIRAKYAGVKVSYKLAEPETREDKEGRLVMVQN
                     EESIVEMTDDQLAEIAALSKEVRNKLISGN"
     gene            2664..3104
                     /locus_tag="PLA17_08590"
     CDS             2664..3104
                     /locus_tag="PLA17_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85513.1"
                     /translation="MKKRLLILTIVLVILPLMASGQPATDDLVSSCVLAAGENTTYLK
                     DFRVQLPKASQDAAVPVYKANMYLMKNMKYRFCVCDSPESGGELFITIYDQGKEIISS
                     YNSSSARKYSSVDFICNKTGLYTLWYSFIGGEQGSGVGVVCMIR"
     gene            complement(3105..4091)
                     /locus_tag="PLA17_08595"
     CDS             complement(3105..4091)
                     /locus_tag="PLA17_08595"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HPK85514.1"
                     /translation="MIRFFRSTGAAPVMILLLAALGLWSIYLISPPGLENLTDPEPMP
                     LWGLIVKLFSGSPQLAVLFSFVLVVTVAIVMIRFNTEIFFIPRRTYLPALLYILLYSV
                     FPGEMILNPALPASLLILAALWRMVSSYRMNGLSFNFFDAALMISSAGLLYAGSVWFL
                     VIVFIGAPLLRSPNARELTLALAGALLPWIVMYAVWYVTGASPGELTGIISHNLFDQS
                     ASVGWSRTLIILLAVVGLCFLLSLLYLFREMPTYKIRSRKTWEIMIWLMVISAAALIF
                     VPSVSVEITAVAAIPVSFVIANYMTFTRRVVMAEVLFWLMVIMLVVSRLWPV"
     gene            complement(4088..5371)
                     /gene="purD"
                     /locus_tag="PLA17_08600"
     CDS             complement(4088..5371)
                     /gene="purD"
                     /locus_tag="PLA17_08600"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPK85515.1"
                     /translation="MNILILGSGAREHAIAWKLAQSSRPLKLFVAPGNPGTAETAVNL
                     PVDPLDFERVREVIIANSIGMVVVGPEAPLAEGIADFITGDPALSGVEVIGPCHDGAM
                     LEGSKDFAKGFMKRYGIPTAAYETFTPGTFNDAVIFMRRMKPPYVLKADGLAAGKGVL
                     IINDFDEAVTGLKAMLGGKFGAAGNKVVIEEYLQGIEMSAFVITDGISYRILPEAKDY
                     KRIGEGDTGKNTGGMGAVSPVPFASSSFLEKVEERIIKPTIEGLKREGIDYRGFIFFG
                     LMNVGGDPYVIEYNARLGDPETEVIIPRIASDLYDLLEGVAQRDLASREIKIVTDTAV
                     TVMMVSGGYPDSYGKGFPVSGVENVTESIIFHAGTTMKDGRLVTSGGRVMAVTSMGST
                     MQEALDKSYSSVREISFNDMSYRRDIGSDLKNHII"
     gene            complement(5419..6045)
                     /locus_tag="PLA17_08605"
     CDS             complement(5419..6045)
                     /locus_tag="PLA17_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK85516.1"
                     /translation="MIRNHNRPFPASVIYPDAIYSIRGRGKKLYLTFDDGPDPESTPR
                     ILDILDAHGAKATFFCTGSKVLASPAIFARIASEGHLAGNHGYSHLNGLKTPVRTYCA
                     DAFRGRDITCSNLFRPPYGTIRRRQYRILERSMQIVFWSIMPYDFDQTLTADESYTIL
                     MRRLKPGSVIVLHDTPSGTALQYLDRFLKNAMDDGWSFGLVDDSLTLA"
     gene            complement(6042..>6804)
                     /locus_tag="PLA17_08610"
     CDS             complement(6042..>6804)
                     /locus_tag="PLA17_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85517.1"
                     /translation="AVRPYHADRMLKPLIWKFSNDMAIKDDLAVMDFIAGNNWERPLY
                     YSVTVPASTYIGLEQHFILEGMAYRVSPIRTDQPVRGETGMVDTREMYENMMNNFRWG
                     NAEKEGVYLDENNRRMFAVFRRQFARLANALVEEGDTVRALAAIEKGLGIVPPEKMPY
                     DYFSTDLVNALALAGRREDAEKLFTEIVDNSVSVLSFIAALPDGKSYGIEYNASVSLQ
                     ALLDVYRLAAGHGMHEVAARASGELNRFYGEMPAR"
BASE COUNT         1797 a         1782 c         1643 g         1582 t
ORIGIN      
        1 tcaagggatt tgacaaggtg ttgatccttg acatgatggg ccagctggta ttctatggga
       61 acccgcttga agcaattgtg cacttcaaaa cccttgaatc acatatcaac tgcggggtcg
      121 ccgaatgtgc ctcatgcggt acggtgaatc cggagacact gttcaatatc ctggagacga
      181 aggtggtgga tgagtacggc aattataccg accggcgtaa agtctcaccg gcagagtggg
      241 ccgaagcata caggaagacc ttgccggaat cgccaccgga agaggagacc gcagcaccat
      301 ggtcaggtct gcaaaagccc ggatggttca gacagacttt catctacctc ttgcgcgacc
      361 tgaaaagcaa gatagccaac aggcaatacc tgctgctgac cctcctggaa ggaccggtcc
      421 tcggactgat tctgtcatac atcatcaggt atattgcaga tccttcatca gatatctacc
      481 tcttcaggga aaatgaaaat atcccgatat acatcttcat gggtattatc gttgccctct
      541 tcctcggact caccatcagc gcagaggaaa tcttccatga ccggaaactg ctgcggagag
      601 agcatttcct tcaacttaac cgaagcagct acctcgtcag caaaatattg atacttgctt
      661 ttatctctgc actccagaca ttcatgttcc tcgccgtggc caatacgatc cttggtatca
      721 gggggctgtt cctttactac tggatcgccc tttttaccac ggcacttgta gccaatatga
      781 tcggactcaa catctcatcg gcactgaatt cagccatagc catctatatt gtcattcccc
      841 tgctgatgat tcccatgatg gtcctgagtg gtgccatgtt cccgtttgac aagctcaaca
      901 ggagcctggc aagggctgac agggtccccg tcatagctga cctcatgcct accaggtgga
      961 cttatgaagc gctgatggtg aaacagttca ccgggaatga gtatggacgg agagtttatc
     1021 cgctgaaaca gcagctgagt gtctctgact tcaataccat ctaccgtatc ccgagggttc
     1081 aggaagcact ggccactctg accgctgttc agggatcagc atccattaca gcagaacagg
     1141 cgaaggagat gcggctgctc aggaatgaaa cagccaccat cggagctgca ggaatcatca
     1201 aacccttcag cggaacagac tcaataactc ctgatctttt caccccgcag ctcgccgcca
     1261 gactgacatc atggctcgcc agtgccgaca gggaatacag gcgattgagc aaccttgcag
     1321 atatgaatct ggataattac atcagcagca ataaaatggc tcttgatctg ttatataata
     1381 actaccataa tgacaagctt gaagatattg tcagaaaggt atatgagaaa aataaaatgc
     1441 ttgagttcaa tgacaggctt atccagaatg ttgaccttat ttactttgag ccggcagaag
     1501 cgggtccgct taacttccgc acccatttca tggcgccggt caagaggttc atgggtctcc
     1561 gggttgacac cttcagattc aatatcctgc tggtgttctc catgataatc gtcctgtatg
     1621 tgaccctcta cacggaatta ctgaagcgaa taatcatgtc ttttgaaaca agcgggcgaa
     1681 aaaagcgaaa tctgaaccgg aactgacctt tttttgagtt tgaaataaat atttactcct
     1741 gatgacaggg aaaataataa aaattgcgac agtgatcctg ctgatgagct tctgctcatg
     1801 caggaacagc agtgtgcgcc agggcgggct gacctttcct gtcgctgact cggtgccggc
     1861 cgccgaggct gaaaaactaa gtgaggaagc agtggcagac attgttcaga acatcgcatc
     1921 tccggtcgag atagccgcca tcctccagtc aatgcagata cccttctccg ccggttacct
     1981 tgcatccaca caggaagctg agaaactgac aaccaacttc cggaaagcag tgatgctcgg
     2041 tatctacggg gctgacctgg gctatctcaa tatttacggg aaaacaggca actcggttga
     2101 cgtactctcg gcaataaagc gcctggctga cggactgcgg gtgggtcagt tctttgactt
     2161 cgagactctg aagcgacttt cagcaagcaa atcaaacctt gactccctgt tgtttctttc
     2221 cgtgaattca tataaccaga ttgacaacta cctcagggat aatgaccggg gttccatttc
     2281 cgcccttatg attacagggg tatggatcga gggacagtac ctggcaaccc aggtggcgct
     2341 aagccatgat gatgaaatac tccgtgaccg cataggtgaa cagaagatta tcctgggtga
     2401 ccttctcatg ctgctgcgcc cttatcgcgc aagcagtgaa gagtacgggt cactttatga
     2461 catgatggaa cagataaggg caaaatacgc tggcgtgaaa gtctcttaca agctggctga
     2521 accggagacc agggaagata aggagggacg gctggtgatg gtgcagaacg aagaaagcat
     2581 agttgagatg accgatgacc agcttgctga aattgcagca ttgtcaaaag aagtcagaaa
     2641 caaactaata tccggcaact aaaatgaaaa agagactttt gattctgaca atagttctgg
     2701 tcattctgcc gctgatggca tccggacagc ctgctacgga tgatcttgta agcagttgtg
     2761 tgcttgcagc cggtgaaaac acaacctatc tcaaggattt cagggtacag ctcccgaaag
     2821 cttcacagga tgcagctgtc ccggtctaca aagccaacat gtacctgatg aagaacatga
     2881 aatacaggtt ctgtgtctgt gactccccgg agtccggcgg tgagcttttc attacaatct
     2941 acgaccaggg caaggagatc atctcatcat ataacagctc atcggccagg aaatacagct
     3001 cggtcgattt tatatgcaac aagaccggac tatacaccct ctggtacagc ttcatcggag
     3061 gagaacaggg ctccggcgtg ggagtggttt gtatgatccg gtgatcagac gggccagagc
     3121 ctcgatacca cgagcataat caccatcagc cagaatagta cctctgccat caccacccgg
     3181 cgggtgaagg tcatatagtt ggcaattacg aaggaaacgg gtatggcagc gacagctgta
     3241 atttccactg atacggatgg cacgaaaatc aatgctgcag cgcttatcac catcagccag
     3301 atcatgatct cccacgtctt tcgcgacctg atcttgtatg ttggcatctc acggaaaaga
     3361 tagagaagtg aaagcaggaa acagaggcca accaccgcca gaagaataat cagggtacgg
     3421 ctccagccaa cagaagctga ctggtcaaac agattatgac tgattatgcc ggtcagctcg
     3481 cccgggcttg ctcctgtgac ataccagacg gcatacatca ctatccacgg caacagggct
     3541 cctgccagtg caagggtcag ttcacgggca ttcggacttc ggagcagcgg ggcacctatg
     3601 aatacaatga ccagaaacca cactgatcct gcatataaca aaccggctga tgaaatcatc
     3661 agggcagcat cgaaaaagtt gaatgacaac ccgttcatac gataggatga taccattcgc
     3721 cagagtgccg ccagaataag cagtgatgca ggcagggcag gattcaggat catttccccg
     3781 gggaaaacgg aatagagaag tatgtagaga agggcaggca gataagtgcg ccgcggaata
     3841 aagaaaatct ccgtgttaaa ccttatcatc acgatggcca cagtcaccac aagtacaaaa
     3901 gaaaagagaa ctgccagctg aggcgatccg gaaaagagct ttacaatcaa tccccagaga
     3961 ggcatcggct ccgggtctgt aagattctcc aggccgggag gagagatcag ataaatgctc
     4021 cataatccga gggcagccag cagaagaatc attaccggag cggctcctgt tgacctgaag
     4081 aacctgatca tattatatgg tttttaaggt cggatcctat atccctcctg taactcatat
     4141 cattgaaact gatttccctg actgaagaat aactcttgtc cagtgcttcc tgcatcgttg
     4201 atcccataga ggttaccgcc atcaccctgc ctcccgaggt aaccagccgg ccatccttca
     4261 tggtggtgcc tgcatgaaaa ataatgctct cagtaacatt ctccacacct gagacaggaa
     4321 agcccttgcc gtaactgtca gggtaacctc ctgacaccat cataaccgtc accgcggtat
     4381 cagtaacgat ttttatttcg cgcgatgcga gatcacgctg tgcaactcct tccagcaggt
     4441 catacagatc actcgctatt cttggtatga tcacctctgt ttcaggatct ccgaggcggg
     4501 cattgtattc aatcacataa ggatcacccc ccacattcat cagcccgaag aatatgaaac
     4561 cccggtaatc aattccctcc cgtttaagtc cctctatcgt gggttttatt atccgttcct
     4621 ccaccttttc aaggaacgag gaggaagcaa aaggcacagg tgacactgca cccatccctc
     4681 cggtattctt tcctgtgtca ccttcgccga tgcgcttgta atccttggct tcaggcagaa
     4741 tccggtatga gatgccgtca gtgatcacga aagcagacat ctctattccc tgaaggtatt
     4801 cctcgatgac caccttgttg cctgctgccc cgaactttcc tccgagcatt gccttcagcc
     4861 cggtgaccgc ctcgtcaaaa tcattgatta tcagcacccc cttccctgct gcaaggccgt
     4921 cagccttaag cacataaggc ggcttcatgc gcctcataaa gatgacagca tcattgaagg
     4981 tgcccggggt gaaggtttca taggcagcag tgggaatccc gtacctcttc atgaacccct
     5041 tggcaaagtc cttgcttcct tcaagcatag caccgtcatg gcacggaccg atcacctcca
     5101 cgccggacag ggcagggtct cccgtgatga aatctgctat cccctcggcc aggggagcct
     5161 cggggccgac aaccaccatg ccgatggagt ttgcaatgat gacttcacga actctttcga
     5221 aatcaagcgg atcaaccgga aggtttacag ctgtttctgc agtgcccgga ttgccgggtg
     5281 cgacaaagag cttcagcggc ctgctgcttt gggccagctt ccatgcaatt gcatgttcac
     5341 gtgctcctga tcccaggata agaatattca tattcaggtc ttttcccatt tcaaaaataa
     5401 catttaggat gaaattactc aggccagggt caaagaatca tccaccagtc cgaatgacca
     5461 gccatcatcc atggcattct tcaggaaacg gtcaagatat tgcagtgcag tgcccgaggg
     5521 ggtgtcatga agcacaatga cactcccggg ctttaacctg cgcatcagta tggtgtaaga
     5581 ctcatctgcc gtaagggtct gatcaaagtc atacggcatt atgctccaga agactatctg
     5641 catgctcctc tccagaatcc tgtattgtct cctgcgtatc gtgccatagg gaggcctgaa
     5701 taaattgctg catgtgatgt ccctccccct gaatgcgtca gcacaataag ttctgacagg
     5761 tgtcttgagc ccgttgaggt ggctgtaacc gtgattgcct gccagatgcc cttcagatgc
     5821 aatcctggca aaaatagccg gggaagcaag gaccttgctt ccggtacaga agaaggttgc
     5881 ttttgcacca tgcgcatcaa gaatatcaag gatgcggggt gtggattcag gatcaggacc
     5941 gtcatcaaag gtcaggtaga gctttttgcc cctgcccctg atgctgtaaa tggcatccgg
     6001 ataaatgaca gatgcaggga aaggcctgtt gtggttcctg atcatcttgc cggcatttct
     6061 ccgtagaatc tgtttagctc tcctgaagcc cttgctgcca cctcatgcat gccatgaccg
     6121 gcagccagcc tgtatacatc aagaagcgcc tgaagtgaga cagaggcatt gtactcaata
     6181 ccgtaactct tcccgtcagg cagggcggca ataaagctga gaactgaaac cgagttgtca
     6241 acaatctcag tgaacagctt ttctgcatcc tccctcctgc ccgcaagggc cagggcattg
     6301 acaaggtcag tactgaaata gtcatagggc atcttttcag gaggcactat gccaagaccc
     6361 ttctcaattg cagccagagc ccttaccgta tcaccctcct ccacaagtgc attggccagc
     6421 ctggcaaact ggcgcctgaa gacggcaaac atgcggcgat tgttctcatc aaggtaaacc
     6481 ccttcctttt cggcattgcc ccaacggaag ttattcatca tgttctcata catctcccgg
     6541 gtgtcaacca tgcctgtctc tcccctgacc ggctgatctg tccgtatagg gctgacccgg
     6601 taagccatac cctcaaggat gaagtgctgc tcaagcccga tgtaggttga ggccggaaca
     6661 gtcaccgagt aatagagcgg acgctcccag ttgttgccgg caatgaaatc catgacagcc
     6721 aggtcatcct tgatagccat gtcattgctg aatttccata tcagcggttt cagcatcctg
     6781 tcagcatgat acggacgcac ggct
//
LOCUS       DAOPBK010000144         6469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_10564_length_6469_cov_4.865295, whole genome shotgun sequence.
ACCESSION   DAOPBK010000144 DAOPBK010000000
VERSION     DAOPBK010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10564_length_6469_cov_4.865295"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2881)
                     /locus_tag="PLA17_08615"
     CDS             complement(<1..2881)
                     /locus_tag="PLA17_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPK85518.1"
                     /translation="MEQTGKLLKYSASAGSGKTHALTAFYLSCVLHDPGAYRRILAVT
                     FTNSAAAEMKERILRRLHHLGLENEKGEKERRVFTEYLCNFFPDLWPDRETAAVTIRK
                     NAPLALNNILQDYSRFTVGTIDSFFQRVIRAFAREMDIPAGYEIELEHEAVLSDAVDS
                     LLAGVATDKKLLEWISSYVASRLDDSKGWDIRKEIMEVAGQVFREDFRQLGADDRKRI
                     GDYGVMAEYASKVFSIRRKFESDLKNLASRGVRIYSDCGLTPDDFLYKSRGGVGESLR
                     RYAQGDTRRPNSNWTKAAVDGKYYAGGAKPDVVNAFANATEMGLDEVVRKISELFERR
                     YSLYLSALAQVRTIHVIGILGAISERVREQAHDQNLFLLSDSGELINKLIADDDTPFI
                     YEKIGAAYDHYIIDEFQDTSRIQWNNFRPLIAETLARGKDNLVVGDIKQSIYRWRNSD
                     WRIIHSEAAQAFGDETVRTVHLSTNYRSRENIILFNNALFSPSSIPALCDERLAFDRL
                     KISEIYEGSKQEGTERRPGGLVSVTLYRKSDDEGWKERVLRDLPSVVERLQDHGYRAD
                     DILLLCRTNEEGKSIISRILEYSSSCTPEQREHYNYEITSGESLLLERNPAVTLMMAC
                     LRYLTDPGSRINRSRIVRSYILATGRNEDLIYSGDGPVESDRIPLAGRQPLPGRPLGC
                     DQTHLEDQTSEPGQPPLPDGPPLDNHTGERDQLPLDNQPMERDERPFDDMTGGGNQSP
                     LPEGWEEKLESFRNSSLYSATEGMIRFFGLGENSANIAYISSFQDVVLTWSGRHSSDI
                     SAFVEWWDGEGCKSTLSQSNRQEAMRVMTVHRAKGLQSRVVIVPFASWEFTKPGFSRP
                     LLWVTEVPAPFDMMPVVLPEINSRLEESLFAEEAMMEKASDWLDGVNLLYVAFTRAVD
                     ALYVMAPETAVKASGSAGAGSLLNQALENLPAGFITRDDEAVRVVE"
     gene            2970..4184
                     /gene="mnmA"
                     /locus_tag="PLA17_08620"
     CDS             2970..4184
                     /gene="mnmA"
                     /locus_tag="PLA17_08620"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPK85519.1"
                     /translation="MKKKVIVGLSGGVDSSVAAWLLKEQGYEVEGIFMINWHDSRGLL
                     KGDCPFDEDITFAEMVARKLKIKLRTTDLSEEYRKRVADYMFSEYAAGRTPNPDVLCN
                     REIKFDSFAREALKHGADFIATGHFCRNELAETPAGRVNRLLAGIDRNKDQSYFLCQV
                     SQHQLDNVLFPVGSLTKPEVRKIAADLGLATAVRKDSQGICFVGKVDLPVFLRQQLGT
                     KKGRIIYMPDGEGFRSWPEGLPGHDATEEELAEMARPHTLNPGDGVTAGVHNGAHFFT
                     VGQRKGLNLGGYREPLFVIGIDTANNIIYAGEGQMHGGLYRKGLFIRSAEVHWIRNDL
                     TMKRGESRDYQVRIRYRQPLQQARLYARQEGYYIIFEKSQRGITPGQFAAWYDGDELL
                     GSGPIDEQSGLI"
     gene            4264..4773
                     /locus_tag="PLA17_08625"
     CDS             4264..4773
                     /locus_tag="PLA17_08625"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HPK85520.1"
                     /translation="MKKLILSLAFVVLAFGLNAQSNVIKTNPLALAFGDFNTTYERVL
                     NNSSSLQISGHYMFKFLSEEVNLGGVGLGYRFYITHAKKPVPAGFHVTPNGVFTFGSV
                     KNEDGTTSGAKTYGFGVELGYQWVWESGFALDLGIGPMYRFLSGSGDESSTASGFWPS
                     ATLAIGYAF"
     gene            complement(4853..5989)
                     /gene="nspC"
                     /locus_tag="PLA17_08630"
     CDS             complement(4853..5989)
                     /gene="nspC"
                     /locus_tag="PLA17_08630"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HPK85521.1"
                     /translation="MIDYSRIPSPCYVIDEERLRKNLGIIKHVADASGAEIILAFKGF
                     AMWSVFPVVREYVSGAAASSIHEARLCYEEMGVPAHTYAPAFRSEEFGTIMRYSSHVT
                     FNSLAQYRKFAPVLQRGGKHISPGLRINPEYSEIAHGIYNPCSPGSRLGVTAGELSGG
                     LPEGVEGLHFHVLFESDSYTLEKVLAVVEKKFGHLLPKLKWLNMGGGHLMTGKNYDTD
                     HLISLLKRFREKHGIHIILEPGSAFAWETGELVTTVEDIIDRDGIKTAILDVSFTAHM
                     PDCLEMPYKPVIIGAFEPVAGKPAYRMGGNSCLSGDFMGDWSFHRELEVGDRLVFWDM
                     IHYTMVKTTTFNGVPHPHIGIWTADQKFRLVREFGYEDYRDRLS"
     gene            complement(5991..>6469)
                     /locus_tag="PLA17_08635"
     CDS             complement(5991..>6469)
                     /locus_tag="PLA17_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPK85522.1"
                     /translation="IKRARFWMTFSQEYLTHLRVIQNIGMARIDPVIYNGVEIIPIQF
                     LKAVLPNPGDLGENYKGWTSIGCRIKGPDKQGRERTYYIWNNCSHEEAYRETGTQAVS
                     YTTGVPAATGAMMYMKGLWKKPGVWNVEQFDPDPFLKELARLGLPWHEKFDIDLEL"
BASE COUNT         1488 a         1718 c         1657 g         1606 t
ORIGIN      
        1 attcgacaac cctgacagcc tcatcgtcgc gcgtaatgaa gccggccgga agattttcga
       61 gagcctgatt aaggaggctt cctgcccctg cgctgccgga agctttcact gctgtctccg
      121 gcgccataac atagagagcg tcaacagccc tggtgaacgc cacataaagc aggttgaccc
      181 cgtcaagcca gtctgatgcc ttctccatca tggcttcttc agcgaacagc gattcctcca
      241 ggcggctgtt tatctccgga agtactacag gcatcatatc aaagggggcg ggtacctcag
      301 taacccagag aagaggtctg ctgaaaccgg gcttcgtaaa ctcccatgaa gcaaatggca
      361 ctatcaccac cctgctttgc aatcccttgg ccctgtgaac cgtcatcacc ctcatcgcct
      421 cctggcggtt cgactggctg agggtgcttt tgcatccctc accgtcccac cattcaacaa
      481 atgccgagat atccgagcta tgccggcctg accatgtgag cacaacgtcc tggaaggagc
      541 tgatatatgc aatatttgca ctgttttcgc ccagaccgaa gaagcggatc atcccttcag
      601 tggcgctata gagtgaagag ttcctgaatg attccagctt ctcctcccac ccttcgggaa
      661 ggggggactg gtttcccccg ccagtcatgt cgtcaaaggg tctctcgtcc cgctccatcg
      721 gctgattatc aagaggtagc tggtctcgct caccagtatg gttatcaagg ggtggcccgt
      781 cgggaagggg aggctggccg ggctcacttg tctggtcctc aaggtgtgtc tggtcgcacc
      841 ccaacggtct gccggggagg ggttgccggc cggcaagggg tatcctgtct gactccaccg
      901 ggccatcacc gctgtagata agatcttcat tccttccggt ggccagaata tatgaccgga
      961 ctatccggct cctgttgatg cggctgccgg gatcggtcag gtatctcagg catgccatca
     1021 tcagcgtcac ggccgggtta cgctcaagga gaagcgactc acctgatgtt atctcatagt
     1081 tgtagtgttc cctctgttca ggtgtgcagg aggagctgta ctcaaggata cggctgataa
     1141 tactcttgcc ctcctcattt gtccggcaga gcaggaggat gtcatcagcg cggtacccgt
     1201 gatcctgcaa ccgctcaaca accgatggca ggtcgcgcag caccctctcc ttccagccct
     1261 catcgtcgct cttccggtaa agtgtcacac tcactaagcc tcccggcctt ctttccgtac
     1321 cctcctgttt ggaaccttca tatatctcgc tgatcttcag cctgtcaaaa gccagtctct
     1381 catcacataa agcggggatt gaagaggggc tgaaaagtgc attgttgaat agtattatat
     1441 tctcacggct gcggtagttg gtgctgaggt gcacagtccg caccgtctcg tctccgaagg
     1501 cctgtgcagc ctcggagtgg attatccgcc agtcactgtt ccgccagcgg tagatcgact
     1561 gtttgatgtc gcccacaacc aggttgtctt tcccgcgtgc gagcgtctcg gctatcagcg
     1621 gccggaagtt gttccactgt atgcgtgatg tatcctggaa ctcatctatt atatagtggt
     1681 cataagctgc accgatcttt tcatagatga agggtgtgtc atcatcagct atcagcttgt
     1741 ttatcagctc acccgaatcg gagagcagga acaggttctg gtcatgagcc tgttccctca
     1801 ccctctcact gattgccccc agtataccta taacatgtat tgtccgtacc tgtgccaggg
     1861 cagagagata taaactgtaa cgcctttcaa acaactctga tatttttctg accacctcat
     1921 caagtcccat ttctgttgcg ttggcgaagg cgtttacaac atcaggcttt gctcctcccg
     1981 cataatattt cccatccaca gcagcttttg tccagttact gtttggccgg cgggtgtcgc
     2041 cctgcgcgta gcgtctcagg ctctcgccaa caccgcccct gcttttatac agaaaatcgt
     2101 ctggagtaag gccacagtca ctgtatattc tgacccccct ggaagccaga tttttcaggt
     2161 cagactcaaa ttttctcctg attgaaaaga cctttgatgc atactctgcc atcaccccgt
     2221 agtcgccgat tcttttccgg tcatcggcac caagctgcct gaagtcctcc ctgaagacct
     2281 gccctgccac ttccatgatt tccttgcgga tatcccatcc cttgctgtca tcaagccgtg
     2341 aggctacata agatgatatc cattcaagca gttttttgtc agtagccact ccggcaagga
     2401 ggctgtcgac agcatcagag aggacagcct catgttccag ttcaatctcg tagccggcgg
     2461 gaatgtccat ttccctcgcg aaagccctga tcaccctctg gaagaatgag tcaatcgttc
     2521 cgaccgtgaa acgtgagtaa tcctgaagaa tattattcag agccaggggt gcatttttcc
     2581 ggatggttac tgcagcggtc tccctgtcag gccacaggtc agggaagaag ttgcagaggt
     2641 attctgtaaa tacccttcgt tccttttcac ccttttcatt ctccaggccc aggtgatgga
     2701 gcctccggag tattcgttcc ttcatctctg ccgctgcaga gtttgtaaag gtgacagcaa
     2761 gtatgcgccg gtatgctccc ggatcatgca ggacgcatga aaggtagaat gcagtgagtg
     2821 catgagtctt tcctgaccct gccgatgcac tgtatttcaa taacttgcct gtctgttcca
     2881 tcttctgaaa agtagtaaaa aaaactgttc aaacggagca gcttcaatgt agttatcaac
     2941 atattgagtg aaagaactac ctttgcgtcg tgaaaaaaaa ggttatcgtc gggctttcgg
     3001 ggggtgttga ctccagtgtc gccgcatggc tgctcaagga acagggctat gaggtggaag
     3061 ggatcttcat gatcaactgg catgatagcc gggggttgct taaaggcgac tgtccctttg
     3121 acgaggatat cacctttgcc gaaatggttg ccaggaagct gaagattaaa ctgagaacga
     3181 ccgacctcag tgaagagtac aggaagaggg ttgccgacta tatgttcagc gagtatgctg
     3241 ccggccgcac acccaatccg gatgtgctct gcaaccggga gatcaagttt gactcatttg
     3301 cccgtgaggc gctgaagcac ggggctgatt tcattgccac cggccacttt tgccgcaatg
     3361 agctggctga aacccctgca ggacgggtaa accgccttct ggcaggaatt gaccgcaaca
     3421 aggaccaaag ttattttctc tgccaggtga gtcagcatca gcttgacaat gtacttttcc
     3481 cggtcgggtc actgacaaag cctgaagtgc gtaagatagc agccgatctg ggtctggcaa
     3541 cggctgtaag gaaggattca caggggatat gctttgtcgg taaggttgac ctgccggtgt
     3601 tcctccggca gcagcttggg acgaagaaag gcaggattat ctatatgcct gacggcgaag
     3661 ggttcaggag ctggcctgag gggctgcccg gtcatgatgc caccgaggag gaactggcgg
     3721 agatggcaag gccccacacg ctgaatcccg gtgatggtgt tacggcaggt gtgcacaacg
     3781 gcgcccattt ctttaccgtg ggacagcgca agggtcttaa cctcggagga taccgggaac
     3841 cgctttttgt gataggcatt gacacagcaa acaatattat ctatgcaggt gaagggcaga
     3901 tgcacggcgg cctttaccgg aagggactct tcatcaggtc agcggaggta cactggataa
     3961 ggaatgatct gacgatgaag agaggtgaga gccgtgatta ccaggttcgt atccgctaca
     4021 ggcagccgct gcagcaggca cgtctctatg cccggcaaga gggttattac atcatctttg
     4081 agaaatcaca gcgtggaata actcccggac agttcgcggc atggtatgac ggggatgaat
     4141 tactcggttc aggtcctatc gatgaacaaa gcggcctgat ttgatgttaa ggttcttgat
     4201 ttatcaacaa tttttgtatt aacttgtcgt tttattttaa agcaggacaa aaaaactaac
     4261 aacatgaaaa aattgattct ttcattggcc ttcgtcgttc ttgcatttgg tttgaatgca
     4321 cagagcaatg taatcaaaac caatcctctt gccctcgcat ttggcgactt caatacgacc
     4381 tatgagagag tactgaacaa cagttcatca cttcagataa gcggacatta catgttcaag
     4441 tttctcagtg aagaggtaaa tcttggcgga gtaggccttg gttaccgttt ttacattaca
     4501 cacgccaaga agcccgtacc ggcaggattt catgttactc ccaacggagt ctttacattc
     4561 ggaagcgtaa agaatgaaga cggaacaacc tccggagcga aaacatacgg ctttggggtt
     4621 gaacttggat atcagtgggt ctgggaaagt ggttttgctc ttgatcttgg aatcgggcca
     4681 atgtaccgtt tcctgagtgg ttcaggagat gagagctcaa cggcctccgg attctggcca
     4741 agcgccaccc tggctattgg ttacgctttc taaagttgta agccatatag aagataagta
     4801 aaggtgtctc aacaggggca cctttttttt ggcaggcgga gagatccgct ggctatgata
     4861 gcctgtcacg gtaatcctca tacccgaact ccctgaccag tctgaacttc tgatctgccg
     4921 tccatatgcc gatatgcggg tgaggcacac cgttgaaggt tgtggtcttg accatcgtat
     4981 agtgaatcat gtcccagaag accaacctgt caccaacctc cagttcgcgg tggaacgacc
     5041 agtctcccat gaaatcgccc gagaggcatg agttgccacc catcctgtag gcgggttttc
     5101 cggcaaccgg ttcgaatgct ccgattatca cgggtttata tggcatctcg agacaatcag
     5161 gcatatgtgc cgtgaatgag acgtcaagga ttgccgtttt gattccgtcg cgatcaatta
     5221 tatcctccac ggtggtaacc agttcgccgg tctcccatgc gaaagcgctg cccggctcaa
     5281 gaataatgtg aatgccgtgc ttctccctga accttttcag gaggctgatc agatggtcag
     5341 tatcgtaatt cttacctgtc atgaggtgac cgccgcccat gttcagccat ttaagcttcg
     5401 gcaacagatg cccgaacttt ttctccacaa cggccagcac cttttcaagg gtgtatgagt
     5461 cagactcgaa cagtacatga aaatgaagac cctccacccc ttcgggtagt ccgccggaga
     5521 gttcgccggc agtaacgcca aggcgcgaac cgggtgaaca gggattataa attccatggg
     5581 cgatctccga atattccgga ttgattcgca gtcccgggga gatatgcttt cccccgcgct
     5641 gcagcacagg agcaaatttc cggtactgtg ccagcgagtt gaatgtgaca tgtgagctgt
     5701 agcgcattat ggtgccaaac tcctcgctgc ggaatgcagg tgcataagtg tgtgccggca
     5761 cacccatctc ctcataacag agcctggctt catgaatgga acttgctgca gcgcctgaga
     5821 catattcacg caccaccggg aatacagacc acatggcgaa acccttgaat gccaggatga
     5881 tttcagcgcc ggaggcatct gcaacatgtt taatgatgcc caggttctta cggagcctct
     5941 cctcgtcaat cacgtagcac ggcgagggga tcctgctgta gtcgatcata tcagagctcg
     6001 aggtctatgt caaacttctc atgccagggc aggccaaggc gggccaactc cttcaggaac
     6061 gggtcaggat caaattgctc cacattccac acaccgggtt tcttccagag gcccttcatg
     6121 tacatcatgg cgcctgtagc tgcaggcacg ccggtagtat agctcacagc ctgtgtcccc
     6181 gtctcacggt atgcctcttc atgactgcag ttgttccaga tatagtatgt acgttccctg
     6241 ccctgtttgt cagggccttt tatccggcag cctattgagg tccatccctt gtagttctct
     6301 ccaagatcac caggattggg cagcacagcc ttgaggaact gtatcggaat tatctccacc
     6361 ccgttgtata tcacagggtc aatgcgcgcc atcccgatgt tctgtatcac ccttaggtga
     6421 gtaagatact cctggctgaa agtcatccag aaacgggccc tctttatcg
//
LOCUS       DAOPBK010000145         6435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_10617_length_6435_cov_4.534483, whole genome shotgun sequence.
ACCESSION   DAOPBK010000145 DAOPBK010000000
VERSION     DAOPBK010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10617_length_6435_cov_4.534483"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PLA17_08640"
     CDS             complement(<1..150)
                     /locus_tag="PLA17_08640"
                     /inference="COORDINATES: protein motif:HMM:NF039908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HPK85523.1"
                     /translation="MFQITILDIIDIIMVAIIFYQLYRLIRGTAAFSIFLGIFFVYLF
                     WLVVKA"
     gene            complement(177..1031)
                     /gene="folP"
                     /locus_tag="PLA17_08645"
     CDS             complement(177..1031)
                     /gene="folP"
                     /locus_tag="PLA17_08645"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPK85524.1"
                     /translation="MNINVRGRLMDFSRPRVMGIINVNDNSFFPGSRYMQDDDIVRAA
                     AMMLADGADILDIGGCSTRPGSEEVPEPVERERVCRAAGLILKKFPEAVISVDTYRSS
                     VAAAAVSDYGAAIINDISGGELDKDMFPLVARLNVPYILTHMQGTPGTMQVNPHYDDV
                     VSEILVWFSQRMKPLKESGVRDIILDPGFGFGKNEDHNFEMLRRFSEFRIAGMPVLAG
                     LSRKTMIWKSLDISPVQALNGTTALNMAALMKGASILRVHDVREARQVVSLFEKMYPE
                     GVQFDHYS"
     gene            1146..1337
                     /locus_tag="PLA17_08650"
     CDS             1146..1337
                     /locus_tag="PLA17_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85525.1"
                     /translation="MKGINCAEDPGVAITVSDTDGKILCMNGNPASTFAKEGEGATVT
                     GPADLSMEIPFKMDHFVRT"
     gene            1357..2046
                     /gene="tmk"
                     /locus_tag="PLA17_08655"
     CDS             1357..2046
                     /gene="tmk"
                     /locus_tag="PLA17_08655"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPK85526.1"
                     /translation="MKLIVIEGLDGAGKSTQISLLKEWFASRQMQSRYIHFPRTEAPF
                     FGELVARFLRGEFGDIGSVDPYLVAMLYAGDRKDAATMIETWLEEKYFVVLDRYTYSN
                     IAYQCAKMNTEDDVRKLKEWILKLEFEYFGIPKPDINIFLDVPFRFTEEKLRDRKVGD
                     KRSYLLGNSDIHESSLEFQKKVRDIYTKVAEDDQSLQVINCHDENNRMPAPEKIFEMI
                     INKLKERNILR"
     gene            2043..3266
                     /locus_tag="PLA17_08660"
     CDS             2043..3266
                     /locus_tag="PLA17_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPK85527.1"
                     /translation="MKLLTQTARILTGLIFTFSGFIKGIDPMGTAFKLGDYFAAFRLG
                     FLDDIALPLSLLLCVVEFVTGMMLLSGALVKPASWMAALFMALFTPLTLVLAIWNPVS
                     DCGCFGDAIVLTNWQTFFKNLIILIPVVFVFITRNDDTGVMSLKAGLNFTLAYVFLFL
                     LFMRMNLAYLPVIDFRPYSIGTNIAEGMAIPHDAVPDKYDIRFIYEKDGVQQEFTLDN
                     YPADDTLWKFIDQKSVLISKGYEPPISGFTLMTEQGVDMTDQVTRQDGDVMLMVARRL
                     DESDRDGLTKGYRLGIELQKRGADFHIVTATPSSEAGPLVAGFSTLYADEITLKTVIR
                     SNPGFVLLHNGTVAAKWSYHNLPEPEKFLDGDLNALALKSRTATGNRLIAISAVMSVL
                     LAVSLALPFRMKKNN"
     gene            3302..4060
                     /gene="tpiA"
                     /locus_tag="PLA17_08665"
     CDS             3302..4060
                     /gene="tpiA"
                     /locus_tag="PLA17_08665"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPK85528.1"
                     /translation="MRKKIVAGNWKMNMNLYEGLELARGLNDHFTTNPVGDREVILCT
                     PFIHLAMVSNLLTAPGLSAGAQNCNDHKSGAYTGEISAAMIKSTGAGNVIIGHSERRT
                     CYGETDELLAAKTGEALRNGLRVIFCCGEVLEERQGNIHFEVVKRQLDRGLFVVSAEE
                     FSRCIIAYEPVWAIGTGVTATPEQAQEMHCYIRRLVRDRYGNDAALDTTILYGGSCKA
                     SNAAELFANPDVDGGLIGGASLVKEEFCSIVNAL"
     gene            4068..>6435
                     /locus_tag="PLA17_08670"
     CDS             4068..>6435
                     /locus_tag="PLA17_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85529.1"
                     /translation="MRKIIITILVAAAVAPALSAQFQHYFSGDTAMFTTELNAFMGTL
                     VSKTEKAEVDLFNRLYDSTVFSRPVKDRIINVASQLRGRRLGQVPGFIHFVRTLTDFV
                     ETEQKQEEVDVWLIGLSEMAFDPRFSNSSLIKFIEVTGLLLIDNTIFSAGTVKWKATD
                     GKVEFARDTVLKINIEAVTLTAYLGRDSTQILNFTGIYYPDIFRLHCKEGIVTWEKAG
                     YDPSKVFARISDFDIDVTKSEFACDSALLTHPTYFQVPVKGKLTDRAVNIISPDKATM
                     PRFETYETRFFIDEIYRGVDYEGGLTLEGAIVRGTGTNWFPASIKLYRNDTLAIKITS
                     RNFILSQNAINSYESSPTLYLGTDSIYHSNLGFSYNTETREVSMSRTTSPLSRSPYFD
                     SYHNMDLYFEYLLWDMDQPTIIMSRTRGASIGSAKFESVSFYNEANFFRLMRLDDVHP
                     LYRVKDYSKEYGFDVFPVEGFAKSIRMPIEQTTALCIELANSGFLFYDRNFNEVAVKP
                     KVDDYIAAFAKKKDYDAITIFSETRDNEENAILNLKDYTIDVRGVTGVSLSDTQNVAI
                     QPYGGRLIIGKNRSISFDGIVYAGLFKIYGKEFSFNYDTFNIRLEKIDSIKISVETGE
                     RDPYGRPVIRQIENMIELATANLSIDDPKNKSGLRSMKQYPIINSTTDSYIFYDEITD
                     LDGLYPKEKFYFKLDPFTYTNIDHYTNKDMALAGEFVGGGIIEPMRQTLTIQPDTSLG
                     FAMTIPPEGIPIFDGRGRLYNHLSMSNSGLIGSGRVEHMTATAVADTFR"
BASE COUNT         1787 a         1546 c         1562 g         1540 t
ORIGIN      
        1 agcttttaca acaagccaga agagatagac gaagaaaatc ccgagaaaaa tgctgaatgc
       61 agcagtgccc ctgataagac ggtacaactg gtagaaaatg attgctacca ttattatatc
      121 gatgatatca aggatagtga tctggaacat atgtcagagt gcagtgactg acaaatttaa
      181 gaataatgat caaattggac gccctccggg tacatctttt caaaaagtga cacaacctgg
      241 cgtgcttcac gaacgtcatg gactctcaga atagaagctc ctttcatgag tgcggccata
      301 ttgagggctg tagtgccgtt gagagcctgc acgggagaga tgtcaagact cttccagatc
      361 atggtcttcc ttgaaaggcc ggcaagaacc ggcatgcccg caatcctgaa ctcggagaac
      421 ctgcgaagca tctcaaaatt gtgatcctca ttcttgccaa acccgaagcc ggggtcaaga
      481 atgatgtccc tgacacctga ctccttcagt ggcttcatgc gttgcgaaaa ccagacaaga
      541 atttctgaga caacatcatc ataatgagga ttaacctgca tagtccctgg cgtaccctgc
      601 atatgcgtaa gaatatatgg cacattcagc ctggcaacaa ggggaaacat gtctttgtca
      661 agctctccgc ccgatatgtc gtttattatt gcagcaccat aatcggaaac ggcagcagca
      721 gccaccgatg acctgtaggt gtcaactgaa ataactgctt caggaaattt cttaagaatc
      781 agccctgcag ccctgcatac ccgttccctc tcaacaggtt caggcacctc ctcagacccg
      841 ggacgggttg aacacccgcc aatatcgagg atatctgcgc catccgcaag catcatggct
      901 gcagccctga caatatcatc atcctgcata tagcggctgc ctggaaaaaa cgagttgtca
      961 ttgacgttaa tgatacccat cacacgaggt ctggaaaaat ccatcagcct gccccttaca
     1021 tttatattca tacctcttct tttattaccg ggcaaatata gtcattggcc ccgtactgtt
     1081 ccctgagtta ttaaccttgc cggcaaatta agtatatttg ctacctgtgg attcagacaa
     1141 atcaaatgaa aggcataaac tgtgctgagg acccgggtgt ggcaattaca gtttctgaca
     1201 ccgatggaaa gatactttgc atgaacggaa atccggcctc cacatttgca aaggagggag
     1261 aaggggccac cgttacggga ccggctgacc tttctatgga gattccgttt aaaatggatc
     1321 actttgtccg tacctgaata acaaatcacc cagaccatga agctaatagt aatagaaggt
     1381 cttgacggcg ccggcaaatc tacccagata tccttgctca aggagtggtt tgcatcaagg
     1441 cagatgcagt caaggtatat tcactttccg cgtactgagg ctcccttctt tggcgaactg
     1501 gttgcccggt tcctgagagg cgagttcgga gacatcggca gcgttgatcc ttaccttgtt
     1561 gccatgcttt acgcaggtga cagaaaagat gcggcaacaa tgatagaaac atggcttgaa
     1621 gagaaatact tcgttgtgct tgatcgttat acttattcaa acattgccta tcagtgcgcc
     1681 aaaatgaaca ctgaggatga tgtaagaaaa ctgaaagagt ggatactgaa actggaattt
     1741 gaatattttg gaatcccgaa accggatatc aatatcttcc ttgacgttcc cttcaggttc
     1801 actgaggaga agctcaggga caggaaggtt ggagataaga ggagttatct ccttggaaac
     1861 agtgatatcc atgagagcag cctcgagttc cagaagaagg tacgcgacat ctacacgaaa
     1921 gtggcggagg atgatcaaag cctgcaggtc ataaactgcc atgatgagaa taaccggatg
     1981 cctgctccgg aaaagatttt tgaaatgatt atcaataaac tcaaggaaag gaatattttg
     2041 agatgaagtt gctgactcag actgcaagga tactcacggg gctgattttt actttctcag
     2101 gctttattaa agggattgac ccgatgggca ccgcattcaa gctgggtgat tactttgcag
     2161 cattcagact gggtttcctt gatgacattg cccttcccct ctctctgctg ctctgcgtcg
     2221 ttgaatttgt aacggggatg atgctccttt caggagcgct ggtgaaaccg gcatcatgga
     2281 tggctgccct gttcatggct ctgttcactc ccctgacact tgttcttgcc atatggaatc
     2341 cggtctctga ctgcggatgc ttcggtgatg caattgtact caccaactgg cagaccttct
     2401 tcaagaatct gatcattctg attccggttg tgttcgtgtt catcactagg aacgatgata
     2461 ccggtgttat gtcactgaaa gccggcctga acttcacact ggcatatgtt ttcctgtttc
     2521 tccttttcat gaggatgaac cttgcatacc ttccggtgat cgatttcagg ccttacagta
     2581 taggaaccaa tattgccgaa ggcatggcca ttccacatga tgccgtccct gacaagtatg
     2641 atatccggtt catatatgaa aaggatggtg tgcaacagga gtttaccctt gacaattacc
     2701 ctgcagatga caccctgtgg aaattcatcg atcagaaatc agtgctcatc tcaaagggat
     2761 acgagcctcc cataagcgga ttcacactca tgacggaaca gggtgttgat atgactgatc
     2821 aggtgacccg gcaggacggc gacgtgatgc tgatggttgc gcgccggctt gatgagtctg
     2881 acagggacgg gctgacgaaa ggttacaggc tgggcattga actgcagaaa agaggcgctg
     2941 acttccatat cgtgacagct acaccctcct cggaagcagg tccgctggtt gcaggattca
     3001 gtacattgta tgccgatgaa ataactttga aaaccgtgat aagatccaac cctggcttcg
     3061 tgcttctgca caacggcacc gtggcagcta aatggtcata ccacaacctc cccgaaccgg
     3121 agaaattcct ggatggagat ctcaatgccc tggccctcaa atcgcgcacc gcaacaggaa
     3181 acaggctgat tgccataagt gctgtaatgt cagttctact ggccgtatca cttgcccttc
     3241 ctttcagaat gaaaaagaac aattgaaaac aacagataag aactttaata attcgactac
     3301 gatgagaaag aaaatagttg caggaaactg gaagatgaat atgaacctgt atgaagggct
     3361 ggagcttgcc agggggttga acgatcattt taccaccaat cccgttggcg acagggaagt
     3421 tatactatgc actccgttta ttcacctggc gatggtcagc aatttgctga cagctccggg
     3481 tctctctgcc ggagcacaga actgcaatga ccacaaatca ggagcttaca ccggagaaat
     3541 ctctgctgca atgatcaaga gcaccggagc aggcaatgtc atcatcggac actctgaaag
     3601 acgaacctgt tacggagaaa cagacgagtt gctggctgcc aagacaggcg aggcactgcg
     3661 aaacggtctc cgggttatat tctgctgcgg cgaagtactt gaagagaggc agggaaatat
     3721 ccactttgaa gtcgtgaaac ggcaactgga tagaggtctg tttgttgttt cagcggaaga
     3781 gttcagcaga tgcatcatcg cctacgaacc tgtatgggct atcggaacag gagtgacagc
     3841 cacccctgaa caggcccagg agatgcactg ctatatccgc cggttggtca gggacagata
     3901 tggcaatgat gcagctcttg acaccaccat actctatggc ggcagttgca aggcatccaa
     3961 tgctgcagaa cttttcgcca atcctgacgt tgacggaggc ctgataggag gagcttcact
     4021 ggtgaaggag gagttctgca gcattgtgaa cgcactctga aaagcccatg aggaagatca
     4081 tcatcaccat tctggttgcc gctgctgtcg ctcctgccct ttcagcacag ttccagcact
     4141 atttctccgg tgacactgcc atgtttacca ctgaactgaa tgcattcatg ggcacactgg
     4201 taagcaagac ggaaaaggct gaggttgatc tttttaacag actgtacgac agtacggtgt
     4261 tttcaagacc cgtcaaggac cggatcatca acgtcgcaag ccagctgcgg gggcgcaggc
     4321 tgggccaggt gcccggcttt atacacttcg tcagaaccct gactgacttc gtggagactg
     4381 aacagaaaca ggaggaggtg gatgtctggc tgattggact aagcgagatg gcctttgatc
     4441 cgcgtttctc aaattcctcc ctgataaaat tcatagaggt gaccggtctc ctgctgatag
     4501 acaacaccat cttttccgca ggaaccgtaa agtggaaagc caccgacgga aaggttgagt
     4561 ttgcgcggga cacggtactt aaaatcaata tcgaagctgt tacgctgact gcgtaccttg
     4621 ggcgtgacag cacacagata ctgaatttca ccggaatata ttaccctgat atcttccggc
     4681 tccattgcaa ggaaggaatt gtcacctggg agaaagccgg ctatgacccg tcaaaggtct
     4741 ttgccaggat atcggatttc gatattgatg taaccaaaag tgaatttgcc tgtgactcgg
     4801 cacttctgac ccacccaaca tatttccagg tgccggtgaa gggtaaactg accgacaggg
     4861 ctgtcaatat catttctcct gacaaggcca caatgccacg cttcgagacg tatgagacaa
     4921 ggttcttcat cgatgagatc tacaggggtg ttgactacga aggcgggctg accctggagg
     4981 gtgcaattgt caggggcacc ggcacaaact ggttcccggc aagcataaaa ctataccgta
     5041 atgacaccct cgcaataaaa attacctcca gaaacttcat cctgtcacaa aacgccatca
     5101 acagctacga gtcgtctccc accctctacc ttggaactga ctccatctat cactcaaacc
     5161 tcggattctc ctataatacc gagacgaggg aggtgagcat gtcacgcacc acaagccccc
     5221 tctcaaggag tccctacttc gactcctacc ataacatgga cctctatttc gagtatctgt
     5281 tgtgggatat ggaccagcct accataatca tgtcaagaac caggggtgca tcaatagggt
     5341 cagcaaagtt cgaatcagtc agcttttaca atgaggcaaa cttcttcagg ctgatgaggc
     5401 ttgatgacgt ccatcccctg tacagggtaa aggattattc caaagagtat ggctttgatg
     5461 tctttcccgt tgagggtttt gcaaaatcca tacgtatgcc catagagcag actacggcct
     5521 tatgcattga gctggcaaac agtggcttcc tgttctatga ccgcaacttc aacgaggtgg
     5581 cggtaaagcc taaggttgat gactatatcg cagccttcgc aaaaaagaag gactatgatg
     5641 cgataaccat cttcagcgag acaagagaca atgaggaaaa tgccatcctg aaccttaagg
     5701 attacaccat tgatgtcagg ggagtaacgg gagtctcgct cagcgacaca cagaacgtgg
     5761 ctatccagcc atacggcggc cgcctgatca ttggcaaaaa cagatcgatc agctttgacg
     5821 gaattgtgta tgccggactt ttcaaaatct atggtaagga gttctcgttc aattatgata
     5881 ccttcaacat aaggctcgag aagatcgatt cgatcaagat ttcagtggag accggggaga
     5941 gagaccctta tggaaggcct gtgataaggc agatcgaaaa catgatcgaa ctcgcgacag
     6001 ccaacctcag cattgatgac ccaaaaaata aatcgggtct cagaagcatg aaacagtatc
     6061 cgattatcaa ttcaaccact gattcctata tcttctatga tgaaataaca gatcttgacg
     6121 ggttgtaccc taaggaaaag ttctacttca agctcgatcc gtttacatat accaatattg
     6181 accattacac caataaggac atggcgcttg caggagagtt tgtcggcgga gggattattg
     6241 aacccatgcg gcagaccctg acaattcagc ccgatacatc gctgggattc gccatgacca
     6301 ttcccccgga ggggattcct attttcgacg gaaggggaag gctgtacaac cacctcagta
     6361 tgagcaactc aggacttatc gggagtggca gggttgaaca tatgacagct acagccgtgg
     6421 ccgacacatt caggt
//
LOCUS       DAOPBK010000146         6425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_10642_length_6425_cov_5.110047, whole genome shotgun sequence.
ACCESSION   DAOPBK010000146 DAOPBK010000000
VERSION     DAOPBK010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10642_length_6425_cov_5.110047"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..710
                     /locus_tag="PLA17_08675"
     CDS             96..710
                     /locus_tag="PLA17_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85530.1"
                     /translation="MVNYLIVLFAVTLIYFASAERLSTYVRLIALQGLLLCGIAIFEL
                     KEVILANLLFIVAETLLFKTMLLPYLLGRIIRKSGVTKVHRQAVPGFYLLLYSIAALL
                     ISVILSVILVNPFINPIYLAIALFSLLTGLLLIVTHKLILSHLVGFLVVENAVFLFSL
                     AIGNEMPMLINIGILLDIFVGVLILGFFGLRLKPHTNELTMLKD"
     gene            716..2140
                     /locus_tag="PLA17_08680"
     CDS             716..2140
                     /locus_tag="PLA17_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPK85531.1"
                     /translation="MLLLFFILSLALSGVTLLSRNRGLTRVFVLAFSALLIAFTVHAW
                     INRGNTEFIWFTYDSTAVLLLSVLTLLSVPTIYHGFIYTRDDTLKRYNIYHSGLIALM
                     TFMAGAYLANTMTVMWIFVEATTLAVAVLIYHDRTEMSLEATWKYVFVCSTGIAVAYL
                     GILFLGFIYGRGDAPNLSFSSFSHLIGNANPLYLKIAFLFVVVGFSTKMGLFPMHTVT
                     IDAHSVAPPPVSALISTTLMNVGFLAIYRVYTLFSGTPILPFMNNVLILAGVFSLLIA
                     AGYMLKAKHLKRMLAYSSLENMGLVAIALGVGGYGYYAALLIIVLHSLAKSSMFYQMG
                     QFSRVLHTFRLDESGRYMKLYPAGAMVLMTGMICILAIPPSGMFISEIMILRAMVVNG
                     QWLVMALTVILLCCIIYAMSTRIMHVSFSSPRQESELPQPGMVSPVETVSQFILLALV
                     IMICFWQPPFLVDLISNGISSLPR"
     gene            2146..3666
                     /locus_tag="PLA17_08685"
     CDS             2146..3666
                     /locus_tag="PLA17_08685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPK85532.1"
                     /translation="MSSNRYKVVRNGSSPAGFSEIPVLGYELFHEEVTSMLADERYHC
                     LNYSCFRSGEGFRFICVIADDGSGEIRILSHIKKDDGTPLRSLAASCYAMHIFEREIY
                     ENHGVLFDDHPWLKPVRYPHDRADRLSVMDNYPFYEMDSEELHEVGVGPIHAGVIEPG
                     HFRFICNGERVLHLEIQLGYQHRGIEKLFVSAGDGLHRALLSENIAGDSAVAHGLAHA
                     QLLESLAGLEPPVSLQIERCIALELERIGVHIGDTAALCGDVAYQLGQVACEALRTMV
                     INTTQFWCGNRFGKGLIRPGGSNFPLTEESAAEILRVLEEVDRRYANVTDCIYSLPSV
                     LGRFEDIGIVTRKQATSIGAVGMAARTCGVPRDIRATHPFQYYRKMEINPVMQETGDV
                     LARGMLRALEVRESVKIIRRLIENWRNCAKETGRPQYDLRFRPDCLAVSVTEGWRGEI
                     CHVAVTDNDGKLVMYKIKDPSMHNWMALALAVRNQEISDFPVCNKSFNLSYCGNDL"
     gene            3684..4439
                     /locus_tag="PLA17_08690"
     CDS             3684..4439
                     /locus_tag="PLA17_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HPK85533.1"
                     /translation="MLKEIKILSDHGKQYVPDLRNQPVSALFRGRPVITADITAAEAR
                     KAAALCPAGAIDSSSGAVDLGRCAFCCECALAMPEVYRFTNDYRISATRREDLIIKPG
                     QSEPLRIDESAVRKEVRRLFRRSLKLRQVSAGGDNSCEMELSAAGNVNFDMGRYGIEF
                     VASPRHADGIVITGPVSENMSAALEICYEATPAPRLIILVGTDAISGGLFAGSPAIKR
                     DFIESQHIDLYVPGNPAHPLTFINGVMDLTGRK"
     gene            complement(4453..5778)
                     /locus_tag="PLA17_08695"
     CDS             complement(4453..5778)
                     /locus_tag="PLA17_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPK85534.1"
                     /translation="MKKVILTLMLLPALMANRVSGQDISNLIRNEVASSVAAAEVMYR
                     DLHQNPELSLMEFNTAAKMADALGRLGFEVTTGVGGNGVVGVLRNGKGPVIMLRADMD
                     ALPVRETTGLPFASTITVATPQGPETPVMHACGHDLHMTVWHGTVQTLVALKSKWKGT
                     LVAIAQPAEEVSGGSDRMIADGLYSRFPKPDYALCYHVSADLPAGTVGYFPGAIFAGV
                     NSADLKVFGVGGHGAMPHKTIDPVVLSAKIILDYQTIVSREINPVYPAVVTVGSIHGG
                     TKHNIIPGEVEMKLTLRFFSDGVYNQITEALVRIANGHALAAGLPEEMMPVITFPEDF
                     TPPVENDANLVGMAVASMENVLGKENVIRVDPATVAEDFGKYGRTDEKVKIALFWLGG
                     VNREKYDESIASGTNLPALHSSNFAPDFVPAYTTGATAMTRAVMDLFNR"
     gene            6085..>6425
                     /locus_tag="PLA17_08700"
     CDS             6085..>6425
                     /locus_tag="PLA17_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPK85535.1"
                     /translation="MGTALLTSCGEAGKKAAPEAEFKYEVDRFEDISVLKYRLPAFEQ
                     LTTGEKEFIYYLSEAALCGRDILWDQNFKYNLLIRKTIEAIIDTYDGDRESDDYKAFI
                     TYARRVFFANG"
BASE COUNT         1523 a         1580 c         1644 g         1678 t
ORIGIN      
        1 acaagaatcc gaagttcatc ctgactttgt cctcaatagc gcttattgcg ttcatggtaa
       61 tactgatact gactgaaaaa ctggcctgaa aggaaatggt aaactacctt atagttctgt
      121 ttgcggtgac tctcatctac tttgcctcgg cagagaggct gagtacgtat gtaaggctta
      181 tagcgctgca gggtcttctg ttatgcggca ttgcaatatt tgaacttaag gaggtcatcc
      241 tggcaaacct gctcttcatt gtggcggaga cgctgctttt caagaccatg cttctgccat
      301 acctgctggg ccggataatc cggaagtcag gtgtgacaaa ggtacacagg caggctgtcc
      361 ccgggtttta cctgctgctc tattccattg cagctcttct gatcagcgtc attctctccg
      421 taatacttgt caacccgttc atcaatccga tatacctggc aatagctctg ttcagccttc
      481 ttacaggatt gctgctgatt gtgacccaca agctcattct ttcgcacctt gtcggattcc
      541 ttgtggttga gaacgctgtc tttctgtttt cactggccat aggtaatgag atgccgatgc
      601 tcatcaacat cggaatactt cttgacatct tcgtgggtgt cctgatcctg ggctttttcg
      661 ggcttcggct caagccgcat acaaatgaac tgacgatgtt aaaggattaa ggataatgct
      721 gttgctattc ttcatactgt cactggcgct gagcggcgtt acactcctct cccggaacag
      781 gggattgaca agggtatttg tcctcgcgtt ctctgccctt ctcattgcct ttacggttca
      841 cgcctggata aacaggggca acaccgagtt catctggttc acctatgaca gtacggcagt
      901 gctgctgttg tcagtactga ccctgctgtc agtgcccaca atttatcatg gctttatcta
      961 tacacgtgat gacaccctta aaagatacaa tatatatcac tccgggctga tagcacttat
     1021 gactttcatg gcaggagcct acctggccaa caccatgacg gtaatgtgga ttttcgtgga
     1081 ggctaccacc cttgccgttg cggtgctcat ctaccatgac cgtaccgaga tgtcacttga
     1141 ggcaacgtgg aagtatgtat tcgtttgttc caccggtata gcagtggctt accttggaat
     1201 cctctttctg ggatttattt acggacgcgg tgatgctccc aacctctcct tctcatcctt
     1261 cagtcacctt atcggtaatg ccaatcccct ttatcttaag attgcattcc tgtttgtggt
     1321 cgttggattc agcaccaaga tgggtctctt ccccatgcat accgtcacca ttgatgccca
     1381 ctccgtggca ccgcctccgg tgagtgcgct tatctccacc accctaatga atgtggggtt
     1441 ccttgccatc tatcgcgttt acacgctttt ttccggcacc ccgatactcc cattcatgaa
     1501 taatgtactt atcctggccg gagtgttttc gttgcttata gctgccggtt acatgctgaa
     1561 ggcaaagcat ctgaagcgta tgcttgcata ttcaagtctt gagaatatgg gacttgttgc
     1621 aattgccctg ggggttggcg gatatggtta ttatgccgct ttgctgatta tagtactgca
     1681 ttctctcgcc aagtcaagca tgttttacca gatgggacag ttttcaaggg tccttcacac
     1741 cttcaggctc gatgaaagcg gcaggtatat gaaactctat cctgcagggg ctatggtgct
     1801 aatgacaggc atgatctgca ttcttgccat acccccgtca ggcatgttca tctctgaaat
     1861 catgatctta agggctatgg tagtaaatgg ccagtggctg gtcatggctt tgaccgtcat
     1921 ccttctctgc tgtataatct atgcgatgag cacgaggata atgcatgtaa gtttttcatc
     1981 accgaggcaa gagagtgaat tgccgcagcc gggcatggtc agcccggtgg agacggtcag
     2041 ccagttcatc ctcctggccc tggtgattat gatctgcttc tggcagccac cctttcttgt
     2101 tgacctcatc agtaacggca tatcatcact tcccagatag aaacaatgag cagcaacagg
     2161 tataaagtag tcaggaacgg ttcatcgccc gcaggatttt ctgagatccc ggtgctcggc
     2221 tatgagctgt tccatgaaga ggtcacctca atgctggcag atgaacgtta tcattgtctg
     2281 aactactcct gtttcaggag cggtgaagga ttcaggttca tctgtgtcat tgccgatgac
     2341 ggttcagggg agataaggat actcagccac ataaagaaag acgatggcac tccgctgcga
     2401 tcgttggcgg catcatgtta cgcgatgcat atatttgaac gtgagatata tgagaatcac
     2461 ggtgtgttgt tcgatgatca tccgtggctc aagccggtga ggtatccgca tgacagggcc
     2521 gacaggctca gtgtgatgga taactatcct ttctatgaga tggatagtga ggagcttcat
     2581 gaggtgggag tggggcccat tcatgcaggt gtgatagagc ccggtcattt ccggtttata
     2641 tgtaatggtg agagggtact gcatcttgaa attcagctgg gttatcagca ccgtggtatt
     2701 gagaagctat ttgtttctgc cggagatggt ctgcacaggg cactcctttc ggaaaacatt
     2761 gccggtgaca gtgccgtggc gcacggactg gcacatgcac aactgcttga atcgctggca
     2821 ggtttagaac ctccggtttc acttcagatc gagcgctgca tagccctgga gcttgaacgc
     2881 attggcgtgc acataggtga cacggctgct ctgtgcggtg atgttgctta ccagctagga
     2941 caggtggcat gcgaggctct gaggacaatg gttataaata caactcagtt ctggtgtgga
     3001 aaccggttcg gtaaaggcct gatacgtccc ggcggatcga acttcccgct cactgaggag
     3061 tccgccgccg aaatactcag ggtactggaa gaggttgaca ggagatacgc taatgtgact
     3121 gattgtatct atagcctgcc aagcgttctc ggacgctttg aggatatagg tattgtaaca
     3181 cggaaacagg caacctccat tggtgcggtg ggtatggctg cgcgcacctg cggggtgccc
     3241 agggatatac gagcaaccca tcctttccag tattacagga agatggagat caatccggtg
     3301 atgcaggaga cgggtgacgt tctcgcccgt ggcatgcttc gtgccctgga ggtcagggag
     3361 tctgtaaaaa taatccgcag gctgattgag aactggagaa attgtgcgaa ggagacaggc
     3421 cggccacagt atgatctgag attcaggcct gactgcctgg ctgtatcggt gacagaaggc
     3481 tggagagggg agatatgcca tgttgcggtt actgacaatg acgggaagct ggtaatgtat
     3541 aagataaagg atccctccat gcataactgg atggcccttg cccttgccgt acggaatcag
     3601 gagatatctg atttcccggt atgcaacaaa agctttaacc tgtcttactg tggtaatgac
     3661 ctgtagataa tatctgaaag gcaatgctga aagagataaa aatactctcc gatcatggca
     3721 aacagtacgt gcctgatcta cggaatcagc ccgtttctgc actgttcagg ggcagaccgg
     3781 tcatcactgc agatattact gcagccgagg ccagaaaggc tgccgcgtta tgcccggcag
     3841 gtgcaattga cagcagcagc ggtgcagttg atcttggaag gtgcgccttc tgctgtgagt
     3901 gtgcgctggc aatgcctgag gtgtacagat ttaccaatga ctaccgtatt tctgctacca
     3961 ggagggaaga tctgatcata aagcccggtc agtctgaacc gctcaggata gatgagagtg
     4021 ctgtcagaaa agaggtacgc aggttgttca ggcgctccct gaaacttcgc caggtgtcag
     4081 caggaggaga caactcatgc gagatggagc tctctgcagc ggggaatgtg aactttgaca
     4141 tgggcagata cggaattgaa tttgtggcat caccccgtca tgctgacgga attgttataa
     4201 ccggtcctgt ttctgaaaac atgtccgctg ccctggaaat ctgctacgaa gcaacccctg
     4261 caccaaggtt aatcatcctt gtaggcactg atgccatcag cggagggctc tttgccggca
     4321 gccctgccat taagagagat tttattgaaa gtcaacatat tgatctgtat gtccccggca
     4381 atcctgccca tccgctcact ttcataaacg gggtgatgga cctgacgggc aggaagtaaa
     4441 tactcagacc tcttacctgt taaacaaatc catgacagcc cgtgtcatgg cagttgcgcc
     4501 ggtggtataa gccggcacga agtcaggggc gaagttactg ctgtgaagtg caggcagatt
     4561 ggttccgctg gcgatactct catcatactt ctccctgttg acaccgccca gccagaagag
     4621 ggcaattttt accttttcat cagtgcggcc atacttgcca aaatcctctg caacggttgc
     4681 cggatcaacc ctgatgacat tctcttttcc aagtacattc tccattgatg ccacggccat
     4741 gccaaccagg ttagcatcat tctcaacagg aggtgtaaag tcttccggaa aggtaataac
     4801 cggcatcatc tcctccggca gaccggcggc cagggcatgg ccgttggcta tgcgtaccag
     4861 cgcctctgtt atctgattgt acacaccgtc ggaaaaaaac cttagtgtca gcttcatctc
     4921 aacctcgcca gggatgatgt tatgctttgt gccgccatgg atagagccta cggttacaac
     4981 cgcagggtaa acggggttga tctcgcggct gacgatggtc tggtaatcga gaatgatttt
     5041 tgcagagagt accacgggat caatggtttt atgaggcata gccccgtggc cgccgactcc
     5101 gaaaacttta aggtcagcag aatttacgcc ggcaaagatc gccccgggga aataaccgac
     5161 ggtaccggct ggcaggtcgg cgctcacatg ataacagaga gcataatcgg gtttcggaaa
     5221 acggctgtag agtccgtcgg caatcatccg gtccgatccg ccgctcacct cctcggcggg
     5281 ctgggctatt gctaccagtg tgcctttcca tttgctctta agcgccacca gtgtttgcac
     5341 tgttccgtgc cagacggtca tgtgaagatc gtgaccgcat gcatgcatca cgggtgtctc
     5401 cgggccctga ggcgttgcaa ccgtaatggt gcttgcgaag ggcaacccgg tcgtctccct
     5461 gacaggcagt gcatccatgt cagcgcggag catgatcacc ggtcccttgc cgtttctcag
     5521 aacgccaaca acgccgttgc cacctacacc ggtggtcact tcaaaaccca gcctgccaag
     5581 tgcatctgcc atctttgccg cggtattaaa ctccatcagt gacagttcgg gattctggtg
     5641 cagatcgcgg tacataacct ctgccgctgc aacagacgag gccacttcat ttcttatcag
     5701 gttgcttata tcctgtccgg ataccctgtt ggccatcagg gcaggcagga gcatgagggt
     5761 caatatcact tttttcatag tatctggctt gatgtctcca aatgtaccaa ttaatactct
     5821 ccgctgtgac cgattccgaa aaatatgata tcatcggttt tgatccgggc tttttgctaa
     5881 cttagttttt ttaaagccct ctccctgtat aacaggaata cgggatgacc aaaagaagca
     5941 taattcagta aacatgaaac tcacatctgt caaatgcaca aacatctgtt tgtctgattt
     6001 tcagtcatgt gggttccatc atgcccttaa aatcaaaact atactataat gaagaactat
     6061 ttattaatcg caacgatttt atttatggga acagcactat tgacctcatg cggagaggcc
     6121 ggcaaaaagg cagccccgga ggctgaattc aaatatgagg ttgacaggtt tgaagatatc
     6181 agtgtgctca agtatcgcct gcccgcgttt gaacaactga cgacagggga aaaggagttt
     6241 atctattacc tgtcggaggc agcactgtgt ggccgggata tactctggga ccagaacttt
     6301 aaatacaacc tgctcatccg caaaaccatc gaggcaataa tcgacacata cgacggtgac
     6361 cgtgagagcg atgattacaa ggcattcata acctatgcca ggagagtctt cttcgccaac
     6421 ggcat
//
LOCUS       DAOPBK010000147         6392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_10701_length_6392_cov_5.758876, whole genome shotgun sequence.
ACCESSION   DAOPBK010000147 DAOPBK010000000
VERSION     DAOPBK010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10701_length_6392_cov_5.758876"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLA17_08705"
     CDS             complement(<1..869)
                     /locus_tag="PLA17_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPK85536.1"
                     /translation="MAIHDNILREITPLTQSDCFTYFYRTKSEFDFPLHYHDEIELNF
                     IRNAKGAKRIIGDHVEEIGNLELALVGPELPHGWFGTGFAPNQVEEVTIQFHKDFFDE
                     KFLQKNQLNFIRDMLRRSLRGIVFSTETTARLEPRIVSLGKRQGFDSVLELLSILHDL
                     SISRDYRLCSDSGFSDTEVFNYNSRRIEKVMEFIHSSFDKQITLGEAARIASMTESAF
                     SRFFKLRTGMTFIDCVTEVRLGHASRMLISTTKTIAEIAYSCGFNNISNFNRIFRRKK
                     SCTPRELRESYDEG"
     gene            1494..2795
                     /locus_tag="PLA17_08710"
     CDS             1494..2795
                     /locus_tag="PLA17_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HPK85537.1"
                     /translation="MKEKLFAGLLMIALAGCGSSQKYDYVTVEDGIFRFAGEPYYFIG
                     TNYWYGAILGTEGEYGDRGRLIRELDHMKSIGITNLRVMVGPEGPDNEPFRVTPALQV
                     APGEYSEMMLDGLDFLLAEMGKRGMHAILFLNNAWDWSGGFSQYLNWNGYGDIPNPNV
                     PPNTWQQFMAFSGQFNTCEPCIRQFEEFIRFILQRTNSYTGVKYTDDPAIMTWEIANE
                     PRAFSDDNIPAFEAMIGRIASLLQELDENHLVTTGTEGRHGCEQKMDVFERIHANPDI
                     DYLVMHIWPKNWGWLDIADIAGTLDSSLVKTNRYINDHLEVAVQLNKPIVLEEFGLPR
                     DLHGFSPDESTVARDTYYGNAFGQVLEHAGRLGPLAGCNIWSYAGEGRAADGRIHWLP
                     GDDYLGDPPQEEQGLNSVFDSDSTIAVISDFNRRLSTFIKK"
     gene            2802..3932
                     /locus_tag="PLA17_08715"
     CDS             2802..3932
                     /locus_tag="PLA17_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPK85538.1"
                     /translation="MRKLLSLTIVILILTSCNTGDSMRSGPADRSATPETVAMLAGLH
                     RTAGEGIMFGHQDDLAYGIGWIYPGGESDVKRVTGDYPAVYGMDLGYIEHRSAVNLDS
                     VPFEDMKTFVQQIWDRGGVITFSWHVDNPLTGESSWDVSSDRVVASVLPGGEKHEEFK
                     AWLDNLAEFLASLRDDQGVAIPVIFRPWHEHTGSWFWWGENLCTTEEFVALWRFTFDY
                     LCGEKNLHNLLFAYSAVGDFKNREHYLERYPGDDYVDLIGFDYYQKGDPGSSAFRENV
                     GRALGMLTEIAVAHGKLAALTETGYEGIPDSTWWTTTFWPTIENHRISYALVWRNAHN
                     LPGHFYAPYPGQLSEQDFISFYNLGETLFQSELTALSIYKQE"
     gene            3945..5129
                     /locus_tag="PLA17_08720"
     CDS             3945..5129
                     /locus_tag="PLA17_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPK85539.1"
                     /translation="MDNFKMRLAKLREEHEALITRRNYKAGTGNGIFDRYAYPVLTGA
                     HAPLEWRYDLNPASNPYLMERFGINAAFNAGAMKFNGKYILAVRVEGYDRKSFFAIAE
                     SPNGIDNFRFWDYPVNMPETEDPDTNVYDMRLTRHEDGWIYGLFCSERKDAKAAPGDL
                     SSAVAAAGIARTHDLVTWERLPDLVSGSQQRNVVLHPEFVNGMYALYTRPQDGFIDAG
                     SGGGIGWALVKDITRAVIAPDDEKIINFRHYHTIKEVKNGEGPHPIKTPAGWLHLAHG
                     VRGCAAGLRYVLYLYMTAPDDPSRVIAEPGGYFMAPVDEERLGDVSNVLFSNGWIADD
                     DGTVYIYYASSDTRMHVAVSSVDRLTDYCMNTPADGFRSAASVKTLNSIIDRNREYLA
                     GK"
     gene            5197..6372
                     /locus_tag="PLA17_08725"
     CDS             5197..6372
                     /locus_tag="PLA17_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPK85540.1"
                     /translation="MLLKEMKAAAKAELVHNILPYWMKNMPDRDGGGFYGQINGRDNV
                     VHGAPRGGILNARILWSFSAAYMKLRDPIYLEMATRAHDYITSYFFDEKHGGTYWSLK
                     SNGEPLDTKKQIYSQAFFIYAMSTYYLALGDRESLDRAIELYRLVEAHSFDRVRGGYL
                     EAFTRGWGEIADLRLSAKDANEKKTMNTHLHVLEAYTALYRVWPDQELRQQLAALIKV
                     FVERIVDPETSHLNLFFDEEWNCRSTIVSYGHDIEASWLLCEAAEALGESDSIRGLAL
                     KIASAASEGLAGDGSLFYEKDDAAGHFDRDRHWWVQAEAVVGFLNAWQLSGDSGWLEL
                     ASDALDYILQNISDPVNGEWHWSIRADGTINLDDDKAGFWKCPYHNTRMCLEILTRQ"
BASE COUNT         1610 a         1542 c         1719 g         1521 t
ORIGIN      
        1 ccctcatcgt agctctcccg gagctcacgg ggggtgcaac tcttcttcct gcggaaaatg
       61 cggttgaagt tggagatgtt gttgaagccg catgagtagg ctatttcagc tattgtcttg
      121 gtggtggaaa tcagcatcct tgaggcatga cccagtctga cttccgtaac acagtcaata
      181 aatgtcatgc cggtgcgcag cttgaaaaac cggctgaagg ctgactctgt catgctggct
      241 atccttgctg cctcgccaag ggttatctgc ttgtcaaacg aactgtgaat gaactccatc
      301 accttctcta tccgcctgct gttgtaattg aacacttcgg tatcactgaa tcctgaatca
      361 gagcataagc ggtaatcacg cgaaattgag agatcatgaa gtatactcaa cagctcaagc
      421 accgagtcaa agccctgcct tttgccgagg ctcactatcc ggggctccag cctggctgtt
      481 gtctccgttg aaaagacaat tccgcgcagt gacctgcgaa gcatgtcacg gataaaattc
      541 agctggtttt tctggaggaa cttctcatcg aagaaatcct tatggaactg aatagtcacc
      601 tcttcaacct ggttgggagc aaagcctgtt ccaaaccagc cgtgtggcag ctcgggccct
      661 accagcgcca gctccaggtt tcctatctct tccacatgat cgccaatgat ccgtttcgca
      721 cccttggcat tcctgatgaa attgagctca atctcatcgt gataatgcag cggaaagtca
      781 aactccgact tcgtcctgta gaaataggtg aaacagtcag actgggtaag gggagtaatc
      841 tctctcagta tattgtcatg tatggccata tcaaaagaag caaggtaaat tcaaagatac
      901 aaaaaagtac caatatatta taaccctggt tcctgaattc tgtttgatgt aacattcatg
      961 aaataagtaa ttaatgaccg gaactatatt gtcatggcat cctgttccgg tgggttaggg
     1021 ataaaattgt atcgtagtgt tgcatatttt gtgttattgt gctggctggc aggctggcag
     1081 gtttttggca aaagacaagg agactcaccg cacaccctgc cgccgtcacg gataaacagg
     1141 ccggcaggtt cttgcgaaca gacaaggata ttcgctgcac actctgtctc cgcagcggcg
     1201 aacaggctgg caggtttttg gcaaaagaca aaggcattcg ccaccctctc ccgccgctgc
     1261 cagagataaa cgggccggca gatttcgcta acagacaaga agtcaattaa ttaaggcgct
     1321 cactttgcaa aagacatcaa tcctgataat caggtagtta tgagcagtca tttgaaaact
     1381 ataccggatt gttgaccgga gataatgaca ttaaagtatc actttttaaa agaatggtat
     1441 taactgccgg cggaggtgat ttcttaattt tgattaagat catatattat cgaatgaaag
     1501 aaaaactatt tgcagggctg ctgatgattg cccttgcagg ttgcggaagc agtcaaaaat
     1561 atgattatgt gacagttgaa gacggtatct tcaggtttgc cggagagcca tactatttta
     1621 tcggaacaaa ttactggtat ggagcaatcc ttggcactga gggagagtat ggtgaccgcg
     1681 ggcgactgat ccgggaactt gatcacatga agagtatcgg tatcaccaac cttcgggtga
     1741 tggttggtcc tgaaggtcct gacaatgagc ctttcagggt cacaccggcg ctgcaggtcg
     1801 ccccgggtga atacagtgaa atgatgcttg acggactcga cttcctgctt gcagaaatgg
     1861 gcaaacgggg gatgcatgcc atcctcttcc tcaacaacgc atgggactgg agcgggggct
     1921 tttcccagta tctgaactgg aacggttacg gtgacatccc aaatccaaat gtgccgccca
     1981 acacctggca gcagttcatg gctttctcag ggcagtttaa cacctgtgag ccgtgcatca
     2041 ggcaattcga ggagttcata cgttttatcc tgcaacgcac aaacagctat accggtgtta
     2101 agtacaccga tgaccctgcg atcatgacat gggagatagc caacgaaccc cgggcattct
     2161 ctgatgataa tatcccggct ttcgaggcaa tgattggccg tattgcttca ttgctgcagg
     2221 agcttgatga aaaccatctt gtgaccaccg gcactgaagg ccgccacggc tgcgagcaga
     2281 agatggatgt ttttgagcgc attcacgcca accccgacat tgattacctg gtgatgcata
     2341 tctggcctaa gaactggggt tggctcgaca ttgcagatat tgccggaacc cttgattcat
     2401 cgctggtgaa aaccaaccgg tatataaatg accatcttga agtggctgta cagctgaaca
     2461 aaccgatagt gcttgaggag ttcggactgc cgcgtgatct ccatgggttc tctcctgatg
     2521 agtcaacggt tgcacgtgat acctattacg gcaacgcctt cggccaggta cttgaacatg
     2581 ccggtcgcct cgggccactg gcaggctgca atatatggtc ctacgcagga gagggcaggg
     2641 ctgcagatgg acgcattcac tggctgcccg gtgatgatta cctcggtgat ccgcctcagg
     2701 aggagcaggg ccttaactcc gtttttgact ccgattccac aattgccgta atctctgatt
     2761 tcaaccggcg tctctcaaca ttcataaaga aataatcacg gatgagaaaa ttattgtcat
     2821 tgaccattgt cattctgata ttgacatcat gcaacacggg tgacagcatg agatcaggtc
     2881 ctgctgacag atcagcaact ccggagacgg tggctatgct ggcaggactg caccgcactg
     2941 ccggtgaagg tatcatgttc ggtcaccagg atgacctcgc ttacggcatt ggatggatct
     3001 atcccggtgg tgaatcagat gtaaaaagag taacaggaga ttacccggct gtttacggaa
     3061 tggacctcgg ctacatagaa cacaggtcag cagtgaacct tgactcagtg ccgtttgagg
     3121 atatgaagac atttgttcaa cagatatggg accgcggggg agtgataacc ttcagctggc
     3181 acgttgataa tccgcttacc ggcgaatcgt cgtgggatgt ctcatcggac agggtggtgg
     3241 cttcggtgct tcccggaggg gagaagcatg aggagttcaa agcctggctt gataacctgg
     3301 cggagttcct ggcatcgctc agggatgatc agggtgttgc aataccggtg atcttccggc
     3361 cctggcatga gcatacggga agctggttct ggtggggaga gaatctttgc acaaccgaag
     3421 agtttgttgc attgtggaga ttcacttttg attacctgtg cggagagaag aaccttcata
     3481 acctgctctt cgcatattcc gccgtgggtg atttcaaaaa cagagaacac tatcttgaac
     3541 gttatccggg agatgactat gttgacctta tcggtttcga ctactaccag aagggtgatc
     3601 cgggtagcag cgcattcagg gagaatgtag gcagggcgct gggcatgctc actgaaatag
     3661 ccgttgccca tggcaaactg gctgcactga cggaaacggg atatgagggc atacccgatt
     3721 caacgtggtg gactaccacg ttctggccga cgatagagaa tcaccgtata tcttacgccc
     3781 tggtatggcg taatgcacac aacctgccgg gacatttcta tgcaccttac ccgggtcagt
     3841 tgagcgaaca ggattttatc agtttttata atctcgggga aactctcttt caaagtgaat
     3901 tgacggccct cagcatctac aaacaggaat aactttttaa ccagatggat aatttcaaaa
     3961 tgcgcctggc aaaactaagg gaagaacatg aggcacttat aacccggcgt aattataaag
     4021 ccggaaccgg taacggcatc tttgacaggt atgcttatcc cgtgcttacc ggcgcccatg
     4081 caccgctgga atggaggtat gacctcaatc ctgcctccaa tccgtacctg atggagcgat
     4141 tcggcatcaa tgccgctttc aatgccgggg caatgaaatt caatggcaaa tatatcctcg
     4201 cagtcagggt ggaaggatac gacaggaaat cattcttcgc cattgccgaa agcccgaacg
     4261 gcattgataa tttcaggttt tgggattatc cggtcaacat gccggaaact gaggacccgg
     4321 acaccaacgt gtatgatatg aggcttacac ggcatgaaga cggctggatt tatggtctct
     4381 tctgcagtga gaggaaggat gcgaaggctg cccctggtga tctctcttca gccgtagccg
     4441 ctgccggcat agcccgcacg catgacctcg tgacatggga acgcctccct gacctggtct
     4501 caggcagtca gcagcgcaat gtggttctcc atcctgaatt tgtgaacggc atgtatgccc
     4561 tctatacccg ccctcaggac ggcttcatcg atgccggcag cggtggcggg atcggctggg
     4621 ccctggtaaa ggatatcacc agggcagtga tagcgccgga cgatgagaag atcattaact
     4681 tcaggcacta tcacaccata aaggaggtga agaacggcga ggggccacac cccatcaaaa
     4741 cccccgcagg gtggctccac cttgcgcacg gggtgagagg ctgcgctgcc ggactgcgtt
     4801 acgttctcta tctctacatg accgcacctg atgacccgtc cagagtgata gccgaaccgg
     4861 gcggatactt catggccccg gtggatgagg agcgcctcgg cgacgtgtca aacgtcctct
     4921 tctcaaatgg gtggatagct gatgatgatg gaacggtcta catctactat gcctcgtcag
     4981 acacgcggat gcacgtggca gtctcatcgg ttgaccgcct gacagattat tgcatgaata
     5041 cgccggccga cggatttcgc tctgcagcat ccgtaaagac gctcaactcg ataattgacc
     5101 ggaacaggga atacctggca gggaagtaga agggtgcaga attaaaagtt atcttaccgc
     5161 caatctaacc tccggcatat gaataatgaa ccaggaatgc ttctgaaaga gatgaaggct
     5221 gctgccaaag cagagctggt tcacaatatt ctgccttact ggatgaagaa tatgccggac
     5281 cgcgatggcg gtggttttta cggtcagatt aacggcaggg ataacgtggt tcacggcgca
     5341 cccaggggcg gcatactgaa tgcccgcata ctgtggagct tctcggcagc atatatgaag
     5401 ctgcgtgatc ccatttacct tgaaatggcc acacgtgcgc acgactatat taccagttat
     5461 tttttcgatg agaaacatgg tggcacatac tggtccctga agagtaacgg tgagccgctg
     5521 gataccaaaa agcagatata ctcccaggcc tttttcatct atgccatgag tacatattac
     5581 cttgccttgg gcgacagaga gtcgcttgac agggcaatag agttatacag actcgttgag
     5641 gcacatagtt ttgacagggt gcggggcggt tacctggagg ctttcacacg tggatgggga
     5701 gagattgctg acctgcgcct cagcgcgaag gatgccaatg agaagaagac gatgaacact
     5761 catctccatg ttcttgaggc atatacggct ctttacaggg tctggccgga tcaggagctc
     5821 aggcagcagc tggcagcact gataaaggta ttcgtggaaa ggatagttga tccggagacc
     5881 tctcacctga atctcttctt tgacgaggag tggaactgcc gctcaacaat cgtctcatac
     5941 ggtcacgaca tagaggcctc gtggctgctt tgcgaggcag ctgaagcgct gggtgagtct
     6001 gacagcataa gggggttggc gctgaagata gcctcggctg ccagtgaggg actggccggc
     6061 gacggcagcc tgttctatga gaaagatgat gctgccggtc attttgaccg cgacaggcac
     6121 tggtgggtgc aagcggaggc ggtggtggga ttcctcaatg catggcagct gagcggcgat
     6181 tccggctggc tggagcttgc ctcggatgca cttgactata tccttcaaaa catttctgac
     6241 cctgtcaacg gcgagtggca ctggagcatt cgtgccgacg gcactatcaa cctcgatgat
     6301 gacaaggcag ggttctggaa gtgcccctat cataataccc gcatgtgtct ggagattctg
     6361 accaggcaat agacagtggt tttggcaaca cc
//
LOCUS       DAOPBK010000148         6264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_10962_length_6264_cov_4.669673, whole genome shotgun sequence.
ACCESSION   DAOPBK010000148 DAOPBK010000000
VERSION     DAOPBK010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10962_length_6264_cov_4.669673"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLA17_08730"
     CDS             complement(<1..349)
                     /locus_tag="PLA17_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85541.1"
                     /translation="MVRLIIPLLLLAIVSCRNRATDETRTAVARAGDRVLYLDQIPSG
                     LLAEGMSREDSTSAVQSYIRQWARKELMALRAEESLTPEYKAEVNRQLNEMRNNLLIY
                     QYQHQMIIQKMDTT"
     gene            complement(352..1842)
                     /gene="guaB"
                     /locus_tag="PLA17_08735"
     CDS             complement(352..1842)
                     /gene="guaB"
                     /locus_tag="PLA17_08735"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPK85542.1"
                     /translation="MSFSEDKLLFEGLTFDDVLLLPAYSEVLPRNVDLRSKFTRNIII
                     NTPIVSAAMDTVTEAELAIAIAREGGIGVIHKNMSVSQQAAQVRKVKRAESVMIFDPV
                     TISDSATVADALALMKEFKIGGIPVIHEDGALEGIVTNRDLRFETNYSRRISEIMTPL
                     ERLVTLSHQTDLAAAAEILQKHKIEKLPIVDEQGRLKGLVTYKDITKAKDRPNACKDD
                     KGRLRVAAAVGIAPNTLSRVEALVGAGADAIAIDTAHGHTKSVIEILREIKKVFPRTE
                     IVVGNIGTAEAARRLVDEGADAVKVGIGPGSICTTRVVAGVGVPQLSAIYNVARAIEG
                     SGIPVIADGGIRYSGDIVKAIAAGADSIMAGSLFAGVEESPGETIIYNGRKFKSYRGM
                     GSVEAMQKGSKDRYFQDMEDDIHKLVPEGITARVPYKGTLTEVIYQLLGGLRSGMGYC
                     GAHDIATLKRDGVFVRITTAGVTESHPHDVTITREAPNYSRKVNEY"
     gene            1970..3880
                     /locus_tag="PLA17_08740"
     CDS             1970..3880
                     /locus_tag="PLA17_08740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPK85543.1"
                     /translation="MLPDIYRETLARYWGFTSFRPMQEEIIESVISGTDVVGLLPTGG
                     GKSLTYQLPPVAGEGLCLVITPLIALMKDQVTRLKTMNIKAMAIHSGMTREEVEISLD
                     NCLYGDYRLLYVSPERLQTQLFRARLPRFNFTLVAVDEAHCISQWGYDFRPSYLHIAE
                     IRDMMNEDVPFLALTATATPRVVEDIISRLRMKNPKVCRTSFRRPNITYIVREVEDKS
                     TYVLRSLKKEQGSGIIYVSSRKRSKEVAETLVANNIKADFYHAGLPQEMRDRKQTSWS
                     AGETRVIVATNAFGMGIDKADVRFVIHWDCPDSVEAYYQESGRAGRDGKPSFSVLLWS
                     QDEKRKLADSVRIKFPPVDRIKDVYEAIGNFYQLAVGSGRNSVHDFDLWKFVSAFRFS
                     VTETYNSLNFLQKEGYLEFTDEINNPSRVHFVVSRDDLYKFQVANEEYDRFIKLLLRT
                     YSGMFSEFVPINEENLAARSGLAREAIYQYLLRLSSQGIIHFIPGKKSPLVIFTEERL
                     DRSHLRISPEHYLKVKENYIERVARITDYAENKTRCRVAFLTAYFGEETGRCGLCDTC
                     QERNELELSKYEFDMIIDKIKELLADKPLRPEELEEQTDLPREKSVKVIRWLLDHQKL
                     VYDNNHRLTWKR"
     gene            4001..4561
                     /locus_tag="PLA17_08745"
     CDS             4001..4561
                     /locus_tag="PLA17_08745"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HPK85544.1"
                     /translation="MEKEEKVIYSRQVAEFAASANEYCKYLESTRDISGIEILKVLQR
                     LLPYIYLRGSLLPLLEPVLEEPNKKTVTEFDWTRMHENLLSKLGSNDPFPVIVSLGDP
                     ADGLYTGSISENLSDIYQDLKDFIVSYKSGNEEVMHDAVWEVLMNFEEFWGKKLLNVL
                     TAIHDVLYSEDDTIADNEQNEDDETV"
     gene            4545..5138
                     /locus_tag="PLA17_08750"
     CDS             4545..5138
                     /locus_tag="PLA17_08750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPK85545.1"
                     /translation="MTKQYNSSITDDELRRLPLLQFDGKVTLVDDMEKFRRVMDEIGR
                     PSLLGFDTETRPAFKKGRRYKVALLQLADADRAWLFRLNMIGLPPELASLLSDGNILK
                     TGVAIRDDLKALRLLAPFEPDGFTDLQGIVADYGIEELGLKKLTAIVLGYNLSKSQQV
                     SNWEAPELTVQQQLYAATDAWACRSIYLALNGNETHQ"
     gene            5148..>6264
                     /locus_tag="PLA17_08755"
     CDS             5148..>6264
                     /locus_tag="PLA17_08755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPK85546.1"
                     /translation="MPANIILKKGKEQSVRRYHPWIFSGAVASVNGNPSEGDVVDVYA
                     WDKTFLATGHWAPGSIAVRIFSFTECNPDRTFFRNRLEEAMTFRINAGIAGRPETTVW
                     RLVHGEGDGLPGLIVDIYGSVAVMQAHTAGFWKIREMIAQLLPEVTGGLVTAVYDKSE
                     GTLPFMARLNPSNGYLVGENSESGAVVTENGYRFRVDWKKGQKTGFFIDQRDSRCLLG
                     RYSNGRRVLNMFGYTGGFSVYAMKNAELVHTVDSSAAATALADENVKLNFGDDPRHRC
                     YTTDAFTFLAEMEYDYDLIILDPPAFAKHQTALNQALQGYRRLNALAIKKIRPGGLLF
                     TFSCSQVVNRDDFRRSVFVAAANTGRNVRILHQTGQPA"
BASE COUNT         1552 a         1650 c         1617 g         1445 t
ORIGIN      
        1 cggtggtatc cattttctgg ataatcatct ggtgctggta ctgatatatc aggagattgt
       61 tcctcatctc attgagctgc ctgttgacct cagccttgta ttcgggagtt aggctctctt
      121 cggcccgcag ggccataagc tctttacggg cccactgcct gatatagctc tgtacggctg
      181 aggtgctgtc ttcccggctc atgccttccg ccagaagccc tgacggtatc tggtcaagat
      241 agagtaccct gtcgccggca cgtgccacag cggttcgggt ctcatcagtg gctctattgc
      301 ggcatgagac aatcgcaagg agcaggagag ggatgataag cctgaccatt atcagtattc
      361 gtttacctta cggctgtagt tgggtgcctc gcgggtgatt gtcacatcat ggggatggct
      421 ctcggtgacg ccggcggtgg ttatcctgac aaaaaccccg tcacgcttga gtgtggctat
      481 gtcatgtgcc ccgcagtaac ccatacctga tcgcagaccg cccagtaact ggtatatcac
      541 ctcggtgagt gtgcccttgt agggaacacg tgccgtgata ccctccggaa caagcttgtg
      601 aatgtcatct tccatgtcct gaaagtacct gtcctttgac cccttctgca ttgcctccac
      661 cgagcccatg ccgcgatagg acttgaattt cctgccgttg taaatgatag tttcaccggg
      721 cgactcctcg acaccggcaa agagtgagcc tgccatgatg ctgtcagctc cggcggcaat
      781 tgccttgaca atgtcacctg aatacctgat gccgccgtca gcgatcacgg gaattccgct
      841 gccttctatg gcgcgtgcca cgttgtatat cgcagacaac tgaggtacac ctacccctgc
      901 aaccaccctc gtggtacaga tggagccggg tccgataccc accttcactg catcagcgcc
      961 ctcatcaaca agcctcctgg ctgcttcggc ggtgccgata ttgccgacaa caatctctgt
     1021 acggggaaat accttcttta tctccctcag aatttctatc acactcttcg tgtgcccgtg
     1081 agctgtgtca atggctatgg cgtcagcacc ggcacctacc agcgcctcaa ccctggagag
     1141 ggtattgggt gcaatgccca ctgcggctgc aaccctcagc cggcccttgt catccttgca
     1201 tgcgttgggc ctgtcttttg cttttgtaat atccttgtag gtcaccagcc ctttcagcct
     1261 tccctgctca tcaacaatgg gaagcttctc tatcttgtgc ttctgaagta tctccgcggc
     1321 agccgccaga tcagtctgat gactgagggt gacaagcctc tcaagcggag tcattatctc
     1381 actgatacga cgcgaataat tagtctcaaa acgaagatca cggttggtga caatgccttc
     1441 cagcgctccg tcttcatgaa ttaccggaat gccgccgatc ttgaattcct tcatcagcgc
     1501 cagggcatca gccactgtgg cactgtcaga aatggtgaca ggatcaaaga tcataacact
     1561 ctctgcacgc tttaccttcc gcacctgggc agcctgctga ctgactgaca tattcttgtg
     1621 aattacgccg ataccgccct cccgggcgat ggctatcgca agctctgcct cggtgacggt
     1681 atccatggcc gccgaaacaa tgggagtatt gatgatgatg ttccgtgtga actttgaccg
     1741 gagatcaaca ttgcgcggta aaacctccga ataggccgga aggagcagca catcatcgaa
     1801 ggtcagacct tcaaacaaaa gtttatcttc agagaatgac atcggacaag tttttttacc
     1861 tggggcgaaa attacaaaaa attgccaagc tcctgcatat cttaaatgtc aattaatggt
     1921 taaaagtaag tttgagttat taaatttgta ttaaacagcc tgcaaaacca tgttgccgga
     1981 tatctacagg gagactctcg cacggtactg gggattcacc tccttcagac ccatgcagga
     2041 ggagatcatt gagtcagtga tctccggaac tgacgtggtg ggccttctcc cgaccggcgg
     2101 cggcaaatcg ctaacctacc agctgccccc cgttgccggt gagggccttt gcctggtcat
     2161 cacacccctg attgcactga tgaaggacca ggtgacaagg ctgaaaacaa tgaatatcaa
     2221 ggcaatggct attcatagcg gcatgacccg cgaagaagtg gagatatccc ttgataactg
     2281 cctgtacggc gattacaggc tgctgtacgt cagccccgaa agacttcaga cacagctgtt
     2341 ccgggcccgc ctgccaagat tcaactttac cctcgtggct gttgacgaag ctcactgcat
     2401 atcacagtgg ggatatgact tcaggccttc atacctccat attgcggaaa tacgggatat
     2461 gatgaacgag gatgtccctt tccttgccct gacggccact gccaccccgc gggtcgtgga
     2521 ggacatcatc tcaagactga ggatgaagaa cccgaaggtc tgccgtacca gcttccggag
     2581 acccaatatc acctatattg tgagggaggt ggaggacaag agtacatacg tcctgcgaag
     2641 cctcaagaag gaacagggca gcggaataat ctatgtgagc agccggaaaa ggtcaaagga
     2701 ggtggctgag actcttgtgg caaacaatat taaggccgat ttctaccatg ccgggctgcc
     2761 acaggagatg cgtgacagga aacagacttc atggagcgcc ggagaaacgc gcgtaattgt
     2821 tgccacgaac gccttcggaa tgggaattga caaggccgat gtcaggttcg tcatacactg
     2881 ggactgccct gactcagtgg aggcatatta ccaggaatca ggtcgggcag gacgtgacgg
     2941 gaaaccttca ttctccgtgc tgctgtggtc acaggatgaa aagagaaagc tggctgactc
     3001 ggtgcgcata aaatttcccc cggttgatcg gataaaggat gtttatgaag ctatcggcaa
     3061 cttctaccag ctcgcggtag gctcaggcag gaacagtgtg catgacttcg acctgtggaa
     3121 gttcgtttcg gccttccggt tctccgtgac tgagacatac aacagcctca atttcctcca
     3181 gaaggaggga taccttgaat ttacggatga gataaacaat ccgtcacggg tacatttcgt
     3241 ggtatcacgc gatgatcttt acaagttcca ggtggccaat gaagagtatg acaggttcat
     3301 caaactgctc ctccgcacct atagcggtat gttctccgaa tttgttccga tcaatgaaga
     3361 aaacctggct gccaggtcag gacttgcccg tgaagctatt taccagtacc ttttgaggct
     3421 ctcatcacag ggaataattc attttatccc ggggaagaag agcccgctgg tcatttttac
     3481 cgaggaaagg cttgaccgaa gccacctccg catctcaccc gaacactacc ttaaggtgaa
     3541 ggaaaactac atagaacggg ttgcacgcat cactgactat gccgaaaaca aaacccgttg
     3601 ccgggtagct ttcctgaccg cttacttcgg agaggagacg ggcagatgcg gactgtgtga
     3661 tacctgccag gagagaaatg aacttgagct gagtaagtac gagttcgata tgatcattga
     3721 taaaataaag gaactgcttg ctgacaaacc cctcaggcct gaggaactgg aagagcagac
     3781 tgacctgcca cgggaaaaaa gcgttaaagt catccgctgg ctccttgacc accagaagct
     3841 ggtctatgac aacaatcaca gacttacctg gaaacggtaa cctgcctcct tttatcaaca
     3901 aatcccccct cacctgcaaa aggtaacccg cctcctttta tcaacaaatc cccggtttta
     3961 agactgaaat gaataatttt gccccggtga tatgttcatt atggaaaaag aggaaaaggt
     4021 tatttattcc cgccaggtgg ctgagtttgc cgcttcagcc aatgagtact gcaaatacct
     4081 ggagtcaacc cgtgatatca gcggtattga gatactcaag gtattgcagc gcctcctgcc
     4141 gtatatatac ctgaggggat cactgctgcc actgctggaa ccggtgcttg aggaacccaa
     4201 caaaaaaacc gtcacggaat tcgactggac ccggatgcat gagaacctgc tttcaaaact
     4261 gggcagcaat gacccgttcc cggtcattgt ctcactgggt gaccccgccg acggactcta
     4321 tacgggaagc atatctgaaa acctgagcga tatataccag gacctgaaag attttattgt
     4381 cagctataaa agcggtaatg aagaggtgat gcatgatgcc gtctgggagg tgctgatgaa
     4441 cttcgaggaa ttctggggaa agaaactgct gaacgtgctt acggcgatcc atgacgtcct
     4501 ctattccgaa gatgacacga ttgctgacaa cgaacagaat gaagatgacg aaacagtata
     4561 acagtagcat taccgatgat gaactgagaa gattgcctct gctgcagttt gacggaaagg
     4621 tgaccctggt tgacgatatg gagaagttcc gcagggttat ggacgaaata ggcagaccct
     4681 cattgctggg atttgatacg gaaaccaggc cggcattcaa aaaggggagg agatacaagg
     4741 tggcgctcct gcagcttgcc gatgctgacc gggcatggct cttcagactg aacatgatcg
     4801 gccttccgcc tgaactggca tcgctgctct ctgacgggaa tatcttaaaa accggtgttg
     4861 ccattcgcga tgacttaaaa gcccttcgat tacttgctcc gtttgaacca gatggattca
     4921 ctgatcttca aggtattgtg gctgactacg ggatagagga gctgggcctc aagaaactaa
     4981 cggccatcgt tctcggatac aatttgtcaa agtcgcagca ggtttcaaac tgggaggccc
     5041 cggaactgac cgtgcaacag caactctacg ctgccacgga tgcctgggcc tgccgcagca
     5101 tttacctcgc cctcaatggg aacgaaactc atcaataaac ctcttcgatg cccgcaaata
     5161 tcatccttaa gaaaggcaag gaacagtcgg tacgccgcta tcacccctgg atcttctccg
     5221 gagctgtagc ttcagtcaac ggaaacccgt cagaaggcga tgtcgttgat gtttatgcat
     5281 gggataaaac attcctggca acggggcact gggctccggg gtctatagca gtacggatat
     5341 tctcctttac tgaatgcaat cctgaccgca cgttcttcag aaaccggctt gaggaggcca
     5401 tgacgttcag gatcaatgcc ggtattgcag gcagacctga aaccaccgtc tggcgtcttg
     5461 tacacggcga aggcgacggg ctgccggggc tgattgttga catctatggc agtgtggcag
     5521 tcatgcaggc tcacaccgcc ggattctgga aaatcaggga gatgattgca caactgctgc
     5581 ctgaggtgac cggcgggctg gtgacagcag tgtacgacaa gagcgaggga acacttccgt
     5641 ttatggcaag gctgaatccc tcaaacggct accttgttgg cgaaaacagt gaatccggtg
     5701 cggttgtcac tgaaaacggc tacagattca gggtcgactg gaagaagggg caaaagacag
     5761 gtttcttcat tgaccagcgt gatagtcgct gcctgctggg ccggtatagc aatggcagaa
     5821 gggtgctgaa tatgtttggc tataccggtg gcttctcggt ctatgcaatg aaaaatgctg
     5881 agcttgtgca tacggtcgac agctctgcgg cggccacggc cctcgcggat gaaaacgtga
     5941 aactcaactt tggcgacgat ccacgccacc gctgctatac cactgacgcc ttcaccttcc
     6001 tggcggaaat ggagtacgat tatgatctta tcatccttga ccctcccgcc ttcgccaaac
     6061 accagaccgc ccttaaccag gcacttcagg gctacaggag actcaatgca ctggctatta
     6121 agaaaatcag gcccggcggt ctgctgttta ccttctcctg ctcccaggtg gtaaaccgcg
     6181 atgacttccg ccgctcggtc ttcgtggccg ccgccaacac cggacgcaac gtgcgcatac
     6241 tgcaccagac cggacagccg gcag
//
LOCUS       DAOPBK010000149         6223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_11060_length_6223_cov_4.506971, whole genome shotgun sequence.
ACCESSION   DAOPBK010000149 DAOPBK010000000
VERSION     DAOPBK010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11060_length_6223_cov_4.506971"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLA17_08760"
     CDS             complement(<1..310)
                     /locus_tag="PLA17_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK85547.1"
                     /translation="MNNTSQNDNVLEIKDLSIAYEPGKYAVNGVTASVKRNSITAIMG
                     PSGCGKSTLLRAINRMHELYPDIQTKGDILLDGQSIFSMSVIELRRKVGMVFQRPNPF
                     P"
     gene            complement(316..1206)
                     /gene="pstA"
                     /locus_tag="PLA17_08765"
     CDS             complement(316..1206)
                     /gene="pstA"
                     /locus_tag="PLA17_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPK85548.1"
                     /translation="MTEINTALTDNIGRRNRTNVRFRYLVIAGAVITVIPIFLIVGSL
                     AIKGFRQINLAFFTQPAPDTFQAMMAASTGDIIPGGIANGLTGSLLMVFLASLFAIPP
                     GLMTGIYIYENSSQKYARFIRSLAEILQGVPSIILGIIAYIWIVKNITKGYSALAGSV
                     SLAIMMLPLIIRSTEETMRMIPVSIREAAFALGTPYYKMIYKILIPVSFSGLATGVLL
                     ALSRILGETAPLMLTALGSPAISFDITKPVSAVPLLIWDFYNDPNMVSLVWSASLFLM
                     VFVLSMNLLARSVASKHKYI"
     gene            complement(1203..2054)
                     /gene="pstC"
                     /locus_tag="PLA17_08770"
     CDS             complement(1203..2054)
                     /gene="pstC"
                     /locus_tag="PLA17_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPK85549.1"
                     /translation="MSGDRVTKALLFISSLTILLLSAGIVISLLGGALPAFKEFGVRF
                     ITSSVWNPTEGSEEYGAMAFIAGTVITSILALIICIPMAFSTSLFIAEYYNGTRTSRI
                     LRSLVDLLAGIPSIVYGFWGFYALRPLLVATGLTDQGFGVFTASLVLAIMIVPYATSL
                     SAEIISMVPAELKEAAYSLGATRREVIFSVVIPSARSGIIASFILALGRALGETMAVT
                     MLIGNANVIPKGLFAPGNTMASVIANQFGEASGLKMSALIAIGLLLFLITALVNYLGK
                     LIIKRFA"
     gene            complement(2059..3123)
                     /gene="pstS"
                     /locus_tag="PLA17_08775"
     CDS             complement(2059..3123)
                     /gene="pstS"
                     /locus_tag="PLA17_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HPK85550.1"
                     /translation="MKKIVFILMAAMALLLVSCGGSGSATGKQKQITLSAAGATFPQP
                     FYNLAFKKFNEAGGPLINYGGIGSGGGIKSLRDQIVNFSATDAFLSDEEMAEMPGEVV
                     HIPTCIGAVVIAYNLPGVTDLKLTPELLEGIFLGNINKWNDPKILASNPGLALPDMEI
                     TVVYRSDGSGTTFIFSDYMSKVSPAWNAQLGTGKALNWPAGIGAKGNPGVSGTISQSQ
                     GSIGYIGYEYAFAQKIPMAAVMNSAGFFIEPTLESFTAAADEEIPSDTRVMLTNSSNP
                     AAYPITSFTWIIMYKELAYGKHSSEAAEAAVKTVRWLIGEEAQAIATSVHYAALPQNA
                     SLQAEELLRGITYQGQTVLK"
     gene            3563..4243
                     /locus_tag="PLA17_08780"
     CDS             3563..4243
                     /locus_tag="PLA17_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85551.1"
                     /translation="MKQIVLTVIVAALMNSAAGQSVESPNYGLKSPLTAEIVRVDFNA
                     DATVVWLTIMSDINNAWFCIERNTFLVRPDGVRLKLTGLKGLPYCPATYKFRRPGEKA
                     SFSLTFPPTGVLPWFSIVEECMGGCLSFRGVVTDAGLNEKLNEAFALSDADNPMAAYR
                     IYEEIINEIDSLNLGIEGSIYTSLIMIDRSMGRNESARRWYNRMMTADTPDLKLYLDN
                     LKRQGVEF"
     gene            complement(4528..5970)
                     /locus_tag="PLA17_08785"
     CDS             complement(4528..5970)
                     /locus_tag="PLA17_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85552.1"
                     /translation="MKRIYSVISALVVTGSLFAGGLVTNTNQSALYTRLQSRNASTGI
                     DAVYYNPAGLTKLSDGFHFSLNNQTIGQTQRVTTAYDPLNGSPKEYVGKVSAPVYPGV
                     YAAFKTGKLALSLGFNPVGGGGGATYEDGLPSFEMPISNLVPMLVQHLTPLDQYIFAN
                     TGTDPNFNGVTGYNSNIYFKGSSIYFGLQANASYKINEMLSVAVGGRYVMASNKYEGY
                     IQDVTITAPEAHGGVQTPGNYLRRVAATPGLPAQNVATLNGVAALLDDATSVEADATM
                     KGSGITPILSVNFAPVGDLINIGLKYEFKTKMTLTTTVNEGKDGNGLFIQDSTAIADM
                     PAMAALGVDIRPSNRLLISGSFNYYFDKGIDYDGQADVEINKIDKNFIEAALGIQVGI
                     TDNLFVSAGWLTTVTGVNENYQSDLKYSLNTNTFGGGLGFKVNNMIDINLGGSYTIYQ
                     EGSKTYAGGNATDIYNKNTWVAAIGLDFHF"
BASE COUNT         1537 a         1459 c         1643 g         1584 t
ORIGIN      
        1 tcgggaaagg gttggggcgc tggaagacca ttcccacctt acggcgaagc tctataactg
       61 acattgaaaa gatactctga ccgtcaagga gaatgtcacc ttttgtctgt atgtcaggat
      121 agagctcgtg catgcggttt atggcccgca gcagggtact cttgccgcaa cccgagggac
      181 ccatgatggc agtaatgctg tttcgcttca ccgaagcggt aacaccattc acggcatact
      241 tcccgggttc ataggcaata gagagatcct ttatctccag tacattatcg ttttgggagg
      301 tgttgttcat caggttcaga tgtatttatg ttttgatgcc acactgcgcg cgaggaggtt
      361 cattgacagc acgaatacca tcaggaacag cgaggcgctc cagaccagac tgaccatgtt
      421 cggatcattg tagaaatccc atatcagcag cggtacggca ctgaccggct ttgtgatatc
      481 gaagctgatg gccgggctgc caagggctgt aagcatcagc ggagccgtct caccaagtat
      541 acgtgacagt gccaggagca ctcccgttgc cagaccgctg aagctgacag gtatgagtat
      601 cttgtaaatc attttgtaat atggagtccc cagtgcaaat gctgcctccc tgattgaaac
      661 gggtatcatg cgcatcgtct cctcggtgga acgtattata agcggcagca tcatgatggc
      721 cagcgaaaca ctccctgcca gtgccgaata accttttgtg atgttcttga ctatccagat
      781 ataggctatg atcccaagta tgattgaggg tacaccctgc agtatctcgg caaggctcct
      841 gatgaaccgg gcatacttct gactgctgtt ttcgtagata tagattccgg tcatcagtcc
      901 gggtggtatg gcgaagagcg acgcaagaaa aaccatcagc agtgatccgg tgaggccgtt
      961 ggctataccg cccgggatga tatcacccgt tgaggcagcc atcatcgcct gaaaggtatc
     1021 gggcgccggc tgggtaaaga atgcgaggtt tatctgccgg aatcccttga tggccaggct
     1081 gcccacaatc aggaagatgg gaatgacggt tatgaccgca ccggctatca ccaggtacct
     1141 gaagcggacg ttggtccggt tacgcctgcc aatattgtca gtcagtgcgg tattgatctc
     1201 tgtcatgcga agcgtttgat tatcagtttg ccgaggtagt tcaccagggc cgttatcaaa
     1261 aagagaagca ggccgatggc tatcagtgca ctcatcttca gtcctgatgc ctcaccaaac
     1321 tggttggcaa tcactgaagc catggtgttt cccggagcaa agagtccttt tggtatgaca
     1381 ttggcattgc caatcagcat cgttacggcc atggtctctc ccagagccct tcccagggcg
     1441 aggatgaagc tggcgataat gcctgaccgg gcagagggga tgaccacgct gaagatcacc
     1501 tcgcgccgtg tggcgccgag tgaataggct gcctccttaa gttcggcggg aaccatcgag
     1561 atgatctccg cactgagcga ggtggcatac ggaacaatca taatagccag caccagtgag
     1621 gcggtgaata ctccgaagcc ctggtcagtc aggcctgtgg ctaccagcag gggacggagg
     1681 gcgtaaaagc cccagaaacc gtaaactatc gaaggaattc cggccagcag gtcaaccagg
     1741 ctccgcagta tcctggaggt gcgggtacca ttgtagtatt cggcaatgaa gagggaggtg
     1801 gagaaggcca tgggtatgca tattatcagc gccaggattg atgtgataac cgtcccggcg
     1861 atgaacgcca tggctccgta ctcttcgctg ccttcggttg gattccagac tgaagaggtg
     1921 atgaatctga ccccgaactc cttaaaggca gggagagccc ctcccaaaag cgagataact
     1981 atccccgcac tgagaagaag tattgtcagt gaagagataa acagtagtgc ctttgtgacc
     2041 ctgtctcctg acatcagttt atttgagaac ggtctgaccc tggtaggtta ttcccctgag
     2101 cagctcttcg gcctgcagcg atgcgttctg cggcagggca gcataatgga cactggtggc
     2161 aatggcctgt gcctcctcgc cgataagcca tctgacggtt ttgactgctg cttcggcagc
     2221 ctcgctgctg tgcttgccat atgccagctc cttgtacatg atgatccatg tgaagctggt
     2281 gatgggatag gcagcaggat ttgacgagtt ggtaagcatc accctggtgt cagacggtat
     2341 ctcttcatcg gcagcggcgg tgaatgattc cagggtaggc tctatgaaga aacctgcgct
     2401 gttcatcact gcagccattg gtattttttg tgcgaaagca tattcataac cgatgtaacc
     2461 gatggagccc tggctctggc tgatggttcc tgaaacgccc ggattgcctt tggcgcctat
     2521 gccggcaggc cagttgagag ctttacctgt cccgagctga gcattccagg ccgggctgac
     2581 ttttgacatg tagtcgctga agatgaatgt ggtaccactg ccgtcagaac ggtagacaac
     2641 ggtaatttcc atatcgggta gggcaagacc cgggttggat gcaagaatct tcggatcatt
     2701 ccacttatta atgttgccca ggaagatccc ttcgagaagt tcaggggtga gcttcaggtc
     2761 tgtgacaccc ggcagattat atgctattac cactgcgccg atgcaggtgg ggatatgaac
     2821 aacctcaccg ggcatctctg ccatttcttc gtctgaaagg aatgcgtctg tagccgagaa
     2881 attaactatc tggtcgcgaa ggctcttgat gcccccgcct gaacctatac ctccgtaatt
     2941 gatgagcgga ccgcctgcct cattgaattt tttgaaggcg aggttgtaga atggctgcgg
     3001 gaaggtggcc ccggcagccg atagggtgat ctgtttctgc ttgccggtgg ctgaaccgct
     3061 gcctccacat gagacgagca gcagtgccat tgcagccatc aggataaaaa caattttttt
     3121 catatttgtt ttattataag tttatgtttt ttctgattca tctatttgca gggttctttc
     3181 cattgccagt gtatttaaga tctgcactct cccgggcggc gcctgctatt gccgttaacg
     3241 tcataatacc gccaacgaca ggtaaaatct tcatcctcat ctgattaata attgacgatt
     3301 tgactacaca aaaatctcac acatttgtta cattcagatt acacaaatgt gacaaagttg
     3361 ttacaagcat gccaatatac cggttatgta gcaggaatca aaagagtgaa aaccgggccg
     3421 gtgcaaatca ttaactttac ggtatgataa ttgcaagtca ggttattata tgaagcacac
     3481 atgaatcaaa tacattggca tgtattaata ttgttttcag tcatgtagat ttcatccggc
     3541 ctttaaaata aattatatac gaatgaagca gatagttcta acagttatag ttgcggcatt
     3601 aatgaacagt gccgccggtc agagcgttga gagccccaat tatggtttga aatcacctct
     3661 tacggcagag atagtgcggg ttgatttcaa tgcagatgcc accgttgtct ggctgacgat
     3721 aatgagtgac atcaacaacg catggttttg catagagagg aacacttttc tcgtgagacc
     3781 agacggtgtc agactgaagc taaccggact gaagggactg ccgtactgtc ctgcaacgta
     3841 caagttcagg cgccccgggg agaaagcttc tttctcactg accttcccgc ccactggagt
     3901 gctgccctgg ttttcaattg ttgaggagtg catgggtggc tgcctctcct tcaggggagt
     3961 tgtgacagat gccgggttga acgaaaagct gaacgaggct ttcgcgcttt cagatgcaga
     4021 caacccgatg gctgcatacc gcatttacga ggagatcatc aatgagattg acagccttaa
     4081 cctgggcatc gagggttcaa tctacacatc cctgataatg attgaccgga gtatgggacg
     4141 gaatgaaagc gccagacgat ggtacaacag gatgatgaca gccgataccc ctgaccttaa
     4201 gctctacctt gataacctga agcgacaggg agttgaattc tgaaagctga aggatatcag
     4261 tcagccgggc ccggtcggat caggataaca ggcctcatgc tggcagtcac ctaataaaaa
     4321 aagagccccg taaaagggct ctctgtatag tataaagaaa aggtcccgta aaggggcttt
     4381 ccgtagtgtg aaaaatggag ccccgtgcga gggttctcct tatagtgtga aagagggagc
     4441 cccgtgtaag ggctctctgt atagtgtaaa aaagagaggc tgcccctttt gcggcagccc
     4501 ctgtttttca taggttaagg tcagttccta gaaatggaag tcgagtccga tggctgcaac
     4561 ccatgtattt ttgttgtata tatcggttgc gtttcctcct gcatatgttt ttgaaccttc
     4621 ctggtatatg gtgtatgagc ctcccaggtt gatgtcaatc atgttattaa ccttgaagcc
     4681 caacccgcca ccgaaggtat ttgtattaag gctgtatttc aggtcgctct gataattttc
     4741 attgaccccg gtaacggtgg tgagccatcc ggcactgacg aagagattgt cggtaatgcc
     4801 tacctgaata ccaagtgctg cctctatgaa gttcttgtca atcttattga tttcaacatc
     4861 agcctgtccg tcatagtcaa tccctttgtc aaagtagtag ttaaagctgc cggagatgag
     4921 gagcctgttt gaaggcctga tgtcaacacc gagtgctgcc atggctggca tgtcagctat
     4981 agctgttgag tcctgaataa acaatccatt accatctttg ccttcattga cggtggtggt
     5041 gagggtcatc ttagtcttga attcgtactt gagtccgata ttgataagat caccaacggg
     5101 agcaaaatta actgaaagga taggggtgat accactgcct ttcattgttg cgtctgcttc
     5161 aactgaagtt gcatcgtcaa gaagggctgc cacaccgttg agagtagcta cattttgtgc
     5221 aggaagcccc ggagtagctg ccactctcct caggtagttg ccgggtgtct ggactcctcc
     5281 gtgagcctcc ggagcagtga tggttacatc ctgaatgtat ccttcgtact tgttgctggc
     5341 cattacatat cggccgccga ctgctaccga gagcatctca ttgatcttgt aggaagcatt
     5401 ggcctgaagg ccgaaataga tagatgatcc cttgaagtaa atgttggagt tataaccggt
     5461 gacaccattg aaattgggat cagttcctgt attcgcgaag atatactgat caagtggagt
     5521 gaggtgctgt acgagcattg ggaccaggtt ggatatcggc atctcaaatg aaggcaaacc
     5581 atcttcatag gttgcgccgc caccgccacc taccgggtta aagcccagtg aaagggcaag
     5641 tttgccggtc ttgaatgcag catatacgcc gggataaacc ggggcgctga cctttccaac
     5701 atactctttc gggcttccgt tcaggggatc gtaagctgtg gtaacacgct gtgtctggcc
     5761 gatggtctgg ttgttgagcg agaagtggaa tccgtcagag agttttgtaa gacctgccgg
     5821 gttgtaatag acagcatcaa tgcctgttga tgcgttgcgg ctctgcagcc tggtgtaaag
     5881 ggcactctgg ttggtattag tgaccaggcc gcctgcgaag agtgagcctg tgacaacgag
     5941 tgcggaaata actgaataga tccttttcat tgacttcttt ttttaattac acattttgag
     6001 ttttactgtg cagtatgcgt aaaagcgccg taaaagtatt aaaaaaactt aaatgtaggg
     6061 atatctggtc gataatatgt gtttctactg taaatggccg tacgataagt caacagttgc
     6121 ctacagattc atcactgcaa acgcacctgt ccgggcagag ctggctgagc caccgcgaat
     6181 atcacactac tgcaaacccg cctatctaat cgtagcaggc tga
//
LOCUS       DAOPBK010000150         6165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_11193_length_6165_cov_5.664321, whole genome shotgun sequence.
ACCESSION   DAOPBK010000150 DAOPBK010000000
VERSION     DAOPBK010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11193_length_6165_cov_5.664321"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLA17_08790"
     CDS             <1..887
                     /locus_tag="PLA17_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK85553.1"
                     /translation="YPAISRFTGSVIEFYKETKWGSYKLPLNDKNTIDWENPMTLGGK
                     VIRIQYSASKENNAEFVLHNYKSAFSKAGYKVLLAIANEELGVGDRPHTWTDRYYEAG
                     GFYNGLNNKKFGLGFNFPTWKSNHSFIVARGKESNKEIYAIVYTVVDDSYTLITLDVV
                     EIEDVETGLVSVDKISGDITNKGHIAIYGIHFEVNKLTIMSESQESLKIIADYLNANK
                     DKKYFIVGHTDNTGDFASNMTLSEERAKSVMSELITKYNVGDSQLKSYGVSSLSPVSS
                     NNTEEGRAKNRRVEIVEQ"
     gene            915..1403
                     /locus_tag="PLA17_08795"
     CDS             915..1403
                     /locus_tag="PLA17_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPK85554.1"
                     /translation="MQHKRLKLSVVFFLGLGLMGLQAQKSVNSTGGNASGSGGSASYS
                     VGQAVYTSNTETGGTVAQGVQQPYEIWVETTIEEAKGINLLVTAYPNPTTDYLTLRID
                     EFDISDLSYQLYDMQGKLLRNEKITSNQTRIVMSNLAPATYSVKVVQGNKEIKTFKIV
                     KH"
     gene            1417..>1909
                     /locus_tag="PLA17_08800"
     CDS             1417..>1909
                     /locus_tag="PLA17_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85555.1"
                     /translation="MKKVFTFLIAVLLTAIVFAQSPEKMSYQAVIRNSNNQLATNQTV
                     GMQISILQGSPDGTAVYVEIQEPVSNANGLVSLEIGSGTVVSGNFSSIDWANGPYFIK
                     TETDLKGGTNYTITGTSQLLSVPYALHAKTAETITSPVTETDPVFDASVASGITATDV
                     SNWN"
     assembly_gap    1910..2009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2010..2929
                     /locus_tag="PLA17_08805"
     CDS             <2010..2929
                     /locus_tag="PLA17_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85556.1"
                     /translation="NWNNKLDSYTETDPVFDASVASGITATDITNWNNKLGSYTETQN
                     LSEVLTLGNDANGIQIKNITDPTDNQDAATKAYVDFLISQIETLGNSNLILWNKLGSI
                     DEVEHSVVGPNGTIVGSVNFNNTVKFSKGITPNTGDAGSGVNFPTTVVDPERGCVEMW
                     VKFYYAPVAYSYGVYGFINVHHWTHNVMLFSWHNDRSLLQFSLQFNGTDRHAELTGFN
                     PALDTPIHIACVWDRTGIDETSEYMRIYVNGGKVAVNDTNNDWGTNNTSGSFRVAAPW
                     DEDFSTDRYTVDDIKIWNYAKTTFDLNKE"
     gene            complement(3250..3323)
                     /locus_tag="PLA17_08810"
     tRNA            complement(3250..3323)
                     /locus_tag="PLA17_08810"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3287..3289),aa:Ala,seq:tgc)
     gene            complement(3334..3410)
                     /locus_tag="PLA17_08815"
     tRNA            complement(3334..3410)
                     /locus_tag="PLA17_08815"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3374..3376),aa:Ile,seq:gat)
     gene            3611..4345
                     /locus_tag="PLA17_08820"
     CDS             3611..4345
                     /locus_tag="PLA17_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPK85557.1"
                     /translation="MANLVLIPTYNERENISGIVRAISGLSVPFEILIIDDNSPDGTA
                     SIVKEMMTSFPNLHLLERPGKMGLGRAYIAGFEWALQKNYNYIFEMDADFSHNPKDLV
                     RLWDACENGGADLAIGSRYISGVNVVNWPLGRVLMSYIASMYVRLVTGMKIMDTTAGF
                     KCYRREVLQNIKPERIKLCGYGFQIEMKFIAWQLGYKIVEVPIIFTDRKAGTSKMSGS
                     IFGEAMWGVLRMKLVSIFKKPTRTVV"
     gene            4356..5714
                     /locus_tag="PLA17_08825"
     CDS             4356..5714
                     /locus_tag="PLA17_08825"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPK85558.1"
                     /translation="MQEIEENQTVIRNALLINEESMRKADVMIAGDCIAGIFPPGKAP
                     SGAGTKIIEASGLWLMPGIIDDHVHFREPGLTHEGDIASESAAAAAGGVTSFMEMPNT
                     LPQTVTISEWEEKNRLAAEKSVINYSFYIGATDSNIDELRRADSSLVPAIKLFMGAST
                     GNMLVSDSSTLEAIFRLRNIPVACHCEDEKIIQANIAGYRAKYGEAVDPSCHPLIRSR
                     EAAIASSQTAAYLARRGGARLHLLHLSTAEETRLLTPGADPAAKQITAEACVHHLWFD
                     DSSYASKGNLVRWNPPVRTAADREALLSAVREGLVDVIATDHAPHTLEEKSAPYFKAP
                     SGGPTVQHSLVMMLELCHRGELTPELVVRKMCHNPALLFGITDRGFVREGYKADLLLA
                     DPAAPWTVNKENILYRCGWSPLEGTTFRSSVITVFANGVPVFEEGRLTGRRAPEMLRF
                     KR"
     gene            5728..>6165
                     /locus_tag="PLA17_08830"
     CDS             5728..>6165
                     /locus_tag="PLA17_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85559.1"
                     /translation="MNRILLPVILIVLLASCGEGSRKKGGASPAGTDATEVADNAITK
                     TLEGTSAANRAGTDSSITLNPGASTDSNTTLNPGAGTDSNTALNPGAGNELPAGAVLI
                     ARDIVTEVIIRPDPEGDPWEVEKVAGYNGGSLVDGIFSSIYDGT"
BASE COUNT         1827 a         1337 c         1437 g         1464 t
ORIGIN      
        1 actaccctgc tatttcccgt ttcacaggtt cagtaattga attttacaaa gaaaccaaat
       61 ggggtagtta taaattgccc ttaaatgaca aaaacactat tgattgggaa aaccccatga
      121 ctttgggagg aaaagttatc cgcatccagt attccgcttc caaagagaat aacgcggaat
      181 ttgttttaca taattacaaa tcagcttttt caaaagcggg ttataaagtt ttgcttgcca
      241 tagctaatga agagcttggt gtgggtgacc gaccacatac atggacagat cggtactatg
      301 aggcaggggg tttttacaac ggtttgaata ataaaaagtt tgggcttgga tttaatttcc
      361 caacttggaa atcaaaccat agttttattg tggctagagg aaaagaaagc aataaagaaa
      421 tttatgcaat tgtctacact gttgttgatg acagctatac tcttattact ttagatgtag
      481 tggaaattga agatgttgag actggcttgg tttcggttga caagatatca ggcgacataa
      541 ctaacaaagg acacattgca atatatggca ttcactttga agttaacaaa ttaacaataa
      601 tgtcagaatc gcaagaatct ctgaaaataa tagctgatta tttaaatgcg aacaaagaca
      661 aaaagtattt tattgttggg catacagaca acacaggtga ttttgcatca aacatgactt
      721 tgtcggaaga aagagcaaaa tcggttatga gtgagttaat aacaaagtat aatgtaggtg
      781 acagccagct gaaaagttac ggagtatcat cattatcacc agtatccagt aataatacag
      841 aggagggcag agcgaaaaac agacgagttg agatcgtaga acaataaaca ttcaattaaa
      901 aaggaaagtc gaaaatgcaa cacaaacgat taaaattaag tgtcgtattc ttcttgggac
      961 ttggactgat gggattgcaa gcacaaaaaa gcgttaactc cacaggcggc aatgcttcgg
     1021 gaagcggagg ctcggcgagc tattccgtcg gacaagcggt ttacaccagc aacactgaaa
     1081 caggcggtac tgtagcacaa ggagtacaac aaccttatga aatttgggta gaaacaacca
     1141 ttgaagaagc caaaggcata aacctgttgg ttacggccta tccaaaccca actaccgatt
     1201 atcttacatt gcgtattgat gaatttgata tttcagattt atcataccaa ttgtatgaca
     1261 tgcagggaaa acttttgcgg aacgaaaaaa tcacaagcaa ccagacaaga attgttatga
     1321 gcaatcttgc acctgctacc tattctgtaa aagttgttca gggaaacaaa gagataaaaa
     1381 catttaaaat cgttaaacat tagggagaac agtagaatga aaaaggtatt tacatttttg
     1441 atagccgtat tattaacggc aattgtattt gcacaatcac ccgaaaaaat gagctatcag
     1501 gcggtaattc gcaacagtaa caaccagttg gcaacaaatc aaactgttgg tatgcaaata
     1561 agcattttgc aaggttcgcc tgatggcaca gctgtttacg tggaaataca ggagcctgtt
     1621 tctaatgcca atggattggt gagtctggaa attggctcgg gtacagttgt ctcaggaaac
     1681 ttctcttcaa ttgactgggc aaacggacct tattttatta aaacagaaac ggatttgaaa
     1741 ggtggcacaa attacaccat taccggcacc agccagttat taagtgtgcc ttatgccctg
     1801 catgcaaaaa ctgccgaaac tattaccagt ccagtaactg aaaccgaccc tgtttttgat
     1861 gcatccgttg caagtggcat aaccgcaact gacgtttcta actggaacan nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc taactggaac aacaaattag acagctacac
     2041 tgaaaccgac cctgtttttg atgcatcagt tgcaagcggt ataaccgcaa ctgacattac
     2101 caactggaac aacaaattag gcagctacac cgaaactcaa aacctctcag aggttttaac
     2161 actgggcaac gatgccaatg ggattcagat taaaaacatt accgacccta cggataacca
     2221 ggatgctgcc accaaagcct atgtagattt tttgatatcc caaatagaaa ctctggggaa
     2281 cagtaatttg atattatgga ataaattggg gtcaatcgat gaagttgaac atagtgtagt
     2341 cggacctaat ggcactattg ttggcagtgt taattttaac aatactgtga agtttagtaa
     2401 aggaattact cctaatactg gtgatgcggg aagtggagtt aattttccaa caacagttgt
     2461 tgatcctgaa agaggttgtg ttgagatgtg ggtaaaattt tactatgccc ctgttgcata
     2521 ttcatatggt gtatatggtt ttatcaatgt tcatcattgg acccataatg taatgttatt
     2581 ttcatggcat aatgaccgta gtttgcttca gttttcatta cagtttaatg gaactgacag
     2641 acatgctgaa ttaacaggtt ttaacccggc tttagatacg cccatacata ttgcatgtgt
     2701 ttgggacaga accggcattg atgaaacttc tgaatacatg aggatttatg taaatggggg
     2761 taaagtagct gtaaatgaca caaataatga ttggggaaca aataacacat ctggcagttt
     2821 cagagtagca gctccatggg atgaagattt ttcaacagac agatatactg ttgatgatat
     2881 taagatatgg aattatgcta aaactacttt tgacttaaat aaagaataaa cacaacaata
     2941 cgccaacaaa gtgccaatat caatggcgct ttgtgacatg acaattgggc actaaagagg
     3001 ggttttgcgg ttgacgtcac ggcctgcccg gctgccgtcg gaaaccccct cttggtgtta
     3061 tgatttttgc ccgagacctg gaaaacaatc tcaagaattg ccggagtaaa tccggaagat
     3121 tgcctgagtc atcgggaata acattttgtc cctttcattc cgtttggaag caaatgtacc
     3181 gttgcaacct tgctgcattg agtcttcgtg caaacaaaaa aagagccctc atttctgaag
     3241 actcttctgt ggagattaag ggagtcgaac ccttgacccc ctgcgtgcaa ggcaggtgct
     3301 ctggccaact gagctaaatc cccgttgctt ttttggtagt cccacccaga gttgaactgg
     3361 ggacctctac attatcagtg tagcgctcta accaactgag ctatgggact gagataagcg
     3421 cacactataa agaacgcccc gattttgaat catcggggtc acaaaagtaa cgcttccttg
     3481 taaacatcca aaatattttc ggaaatattt ttccggccct cacaaaagca tagcctgagc
     3541 ctcaggttca gcacgcttct gcaggacaga gtaataaaaa taattaaatt cgcatttata
     3601 gcagatcaat atggcaaatc ttgtcttaat acccacatac aacgagcggg agaacatctc
     3661 cggaatcgtc agggcaatat ccggtctgtc agtgcctttt gagatactga tcatagacga
     3721 taactctcct gacggaacag cctcaatagt caaggagatg atgacctcgt ttcccaacct
     3781 ccaccttctt gaaagacccg gcaagatggg gctcggccgg gcttatatcg caggcttcga
     3841 atgggcactg cagaaaaact acaattacat attcgagatg gatgccgact tctcccataa
     3901 tccaaaagac ctcgtcaggc tctgggatgc ctgtgagaac ggtggcgccg acctggcaat
     3961 cggatcaaga tacatatccg gcgtaaacgt cgtgaactgg ccattgggaa gggttcttat
     4021 gtcttacatc gcctctatgt acgtcaggct ggtgaccggc atgaagatca tggatacaac
     4081 agcgggtttt aaatgctacc gccgcgaggt actgcagaat ataaaaccgg aacgtataaa
     4141 gctttgcggc tacggtttcc agattgagat gaaattcatt gcatggcagt tgggatataa
     4201 aatcgttgag gtgcccatta tcttcactga ccggaaagct ggcacctcaa aaatgtcagg
     4261 aagcatcttc ggcgaagcca tgtggggtgt tctccgtatg aaacttgtca gtatcttcaa
     4321 gaaaccgacc aggacagtag tctgaccctc ttatcatgca ggaaatagaa gagaaccaaa
     4381 ctgttatccg caacgcactg ctcatcaatg aagagagcat gcggaaggcg gatgtcatga
     4441 ttgcgggtga ctgcatagcc ggaatttttc ctcccggcaa ggcgccctca ggagccggga
     4501 caaagatcat tgaagcatca ggattatggc tcatgcccgg catcattgat gatcacgtcc
     4561 atttcaggga gccggggctg acgcacgagg gtgacattgc cagcgagagt gctgccgctg
     4621 cagccggagg ggtgacatca ttcatggaga tgccaaacac cctgccacag acggttacca
     4681 tcagtgagtg ggaagaaaaa aacaggctgg cagcagaaaa atccgtaatc aattactcat
     4741 tctacatagg agcaactgac agcaacatag acgagctccg cagggcagat tcatccctgg
     4801 tgcctgccat caagctcttt atgggcgctt ccaccggcaa catgctggta agtgacagct
     4861 caacgctgga agcaatattc agactcagga atatcccggt ggcctgccat tgcgaggatg
     4921 aaaagatcat acaggcaaat attgccggat accgtgctaa atacggcgaa gccgttgatc
     4981 cctcctgcca tccgctgatc agaagccgcg aggcagccat tgcctcatca caaaccgcag
     5041 cataccttgc ccgccgtggc ggagcgaggc tccacctgct tcacctgtcg actgcagagg
     5101 agacacggct gctgacaccc ggagctgatc ctgccgcgaa acagattaca gctgaagcat
     5161 gtgttcatca cctctggttt gatgacagct cttatgcctc caaaggaaac ctggtcagat
     5221 ggaatccccc ggtgcgcacc gcagctgaca gggaggcgct gttaagcgca gtgagggaag
     5281 ggctcgttga cgttattgcc acagatcatg ctccccacac tcttgaggag aagagcgctc
     5341 cctatttcaa ggcgccttcg ggcggaccca ctgtacagca ctccctggtg atgatgctgg
     5401 agctgtgcca tcgcggtgag ctcactccgg agctggtggt gaggaagatg tgccataacc
     5461 cggcattgct cttcggcatc actgaccggg gctttgtaag ggagggatat aaggccgacc
     5521 tgctcctggc agatccggcc gcaccgtgga ctgtcaacaa ggagaacatc ctctacaggt
     5581 gcggatggtc gccactggaa ggaaccactt tcagaagcag cgtcataaca gtatttgcaa
     5641 acggggtacc ggtatttgag gaaggcaggc tgacaggccg ccgggctccg gagatgctcc
     5701 ggttcaaaag ataaatagag agacagtatg aacagaattc ttttgccagt catactcatt
     5761 gtacttctcg cctcctgcgg tgagggaagc aggaaaaagg gaggggcctc ccctgccggt
     5821 acggatgcaa ctgaggtcgc agataacgcc atcacaaaaa ccctggaagg aacctcagcg
     5881 gcgaaccgtg ccggtacaga cagcagcatc acactgaacc ccggtgccag cacagacagc
     5941 aacaccacac tgaaccccgg cgccggtact gacagcaata ccgcactgaa ccccggcgcc
     6001 ggtaatgaac tccctgccgg cgcagtactc attgccagag acatagtcac cgaagtaatc
     6061 atcaggcccg atcctgaagg tgatccgtgg gaggtggaaa aggtggcagg ttacaacgga
     6121 gggtcacttg ttgacggtat cttcagcagt atctatgacg gcacc
//
LOCUS       DAOPBK010000151         6096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_11360_length_6096_cov_4.217514, whole genome shotgun sequence.
ACCESSION   DAOPBK010000151 DAOPBK010000000
VERSION     DAOPBK010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11360_length_6096_cov_4.217514"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2038
                     /locus_tag="PLA17_08835"
     CDS             <1..2038
                     /locus_tag="PLA17_08835"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPK85560.1"
                     /translation="KLTAEDIYSINRASMKDAVVLFGGGCTGEFISDKGLIITNHHCG
                     YGYIQNHSTLEHDYLRDGFWAASNAEELANPGLSVTILKYMEDVTDKVLKGVTDDMDK
                     EKRDEIINANITAINEEAVRGTHYRSSVRPFFLGNQYFLIVNEIFRDVRLVGAPPSAI
                     GKFGGDTDNWVWPRHTGDFSLFRVYADKENKPAAYSEENLPYKPAYHFPVSIAGVKEG
                     DFTMVFGYPGRTQEYAPSYHISMLKDVVYPKTVEIRGRKISIMEQAMANDPLIRLKYS
                     GKSFGIANGWKRSIGEIQSLDKMNGVARKQAFEKEFMEWVSADPERLRRYGKILDEYA
                     EIYGSYTTNYFVNSYTNEVFGSTGVEPASLAGAFKRAVEMASGKESGLDNELKRVQEY
                     ADGFFRNYDRNVSEKLFVAVMELYGKNIDSEWQTDVYKALAESCRGDFGMVAGKLFDE
                     SIFTDENRVRALIGNFDPNVVQEDIFYRLAVSASDLIASRLTEELSVANAMLAELNKL
                     YMAAQMEQGVDKVFYPDANSTLRLAYGKVMGYESRDAVYHEHQTTLKGVMEKDNPEIY
                     DYDVPDRLKELYEKKDFGRYGFNGNMPVCFIANNHTTGGNSGSPVLNAEGHLIGVNFD
                     RAWEGVASDMMYNPEQSRNISIDIRYALFLIEKFGNAGYLIDEMTIVGVPQQTP"
     gene            complement(2118..4214)
                     /locus_tag="PLA17_08840"
     CDS             complement(2118..4214)
                     /locus_tag="PLA17_08840"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK85561.1"
                     /translation="MNARMNVPERVSTTILLLILLTGSVAAVSPRVGKGVLDLSEIEQ
                     GDEFAVRLNGEWEFYFGTFIYGTPGTVCDTLIPDCYAKVPGYWSDYTVDGRKLPRFGY
                     GTYRALIVLPRGYRDRMGFDMPVFDTSYEISINGVTMARNGTPARTRAESVPSYKPLI
                     FSYVPKSDTLELLIRVSNYEHRRGGFWLPVKAGTFHSIQNNFSNQWLFSTLVSGILIA
                     SFLFFLIFYLLDRRDRKLLMFAILVLCLALRPFFSAPYLATVVDIRNWNLIIRGEYII
                     LLFMITSGMWLACLIYPARWFRKVSLAVDIVFIPWIAAVVFLPVRIFSWSVFGIQALA
                     ILTLLYAVSMSIRGIMKGNRRDIMYLLAIMAILFGVASDILLSNALEEKQQLYILSFL
                     ILIFVLIQSTLLIADWVRSDKEREKLSLQLEELNRSLESRVDERTRELTDSTEELNAR
                     NKQIALQNKKLTETISLKNKVFSVIAHDLRSPVVNILYTLYLLKEEEFRDRTESLADS
                     CIQYSQQLISLLENMLVWGRGQEDMIKYAPAENDLADIILTNISILKDSADRKNIELN
                     FTQVGRSNGWFDRDLVDIIIRNLLTNAIKYSYHGGKVTIHVKEREESSEWVTISICDN
                     GVGITPERQKRLFTGHEIDSTPGTDSEKGTGIGLKLVYELVALSKGTITVESTPGSGT
                     CFTVILPGSAQAFRIR"
     gene            complement(4224..>6096)
                     /locus_tag="PLA17_08845"
     CDS             complement(4224..>6096)
                     /locus_tag="PLA17_08845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPK85562.1"
                     /translation="PTFDNTIRAMQFTGKLLQKVRRAWSPLTGANTNDSIQALQREMS
                     PLFSKHSDDISLNEKLFERVKSVYENRDKFNLTPEESKLLEDQYLGFIRNGIGLSPED
                     KEVLRKLNSELSLLGVRFGENLLAETNAFTLVIENEEDLSGIPEGIRAQAAAAASAKE
                     MEGKWLFTLQNPSYIPFMTYADNRALREKMFRGYSMRGNNGNENDNNKILADIVRLRV
                     EKARLLGYESHADYVLERNMAKEPETVLNFLSQIWKAALPVAKAEAAAQQALIDREGG
                     NFKLEPWDWFYYTEKIRKEKYDLSDEETKPYFSASNVTEGMFYVANRLYGLEFSERND
                     IPLYHPDVKTWEVKRDNKHVGILMIDYHPRASKRGGAWCSGLRPQRIDENGNFVTPLV
                     SMVTNFTPPAGDTPSLLTTDEASTLFHEFGHALHNLLSNTEYTSGVSRDFVELPSQVM
                     EHWVFEPEVLKVYAKHYQTGEIIPDALIEKIEKAGKFNQGFITVEYLAASSLDMEYHS
                     LTEPVELNIGEFEKKAMEKYGLIKEIIPRYRSTYFNHIIGGYSAGYYSYIWCEQLDAD
                     AFKAFKETGDIFNKEVAARFENEILSRKGSKDELEMWIAFRGREPGIDALLENRGFK"
BASE COUNT         1469 a         1515 c         1577 g         1535 t
ORIGIN      
        1 caagctgaca gctgaagata tctacagtat caacagggcc tcaatgaagg atgccgtggt
       61 gctcttcggg ggaggttgca ccggcgagtt tatctctgat aaaggtctga taatcaccaa
      121 ccaccactgt ggttatgggt acattcagaa ccactccacc ctggagcatg actacctgag
      181 agatggcttc tgggctgcca gcaatgctga agagcttgca aatcccgggc tctccgtcac
      241 catcctcaag tatatggaag atgtcaccga caaggttctc aaaggggtga ctgatgatat
      301 ggacaaggag aaacgtgatg agatcatcaa tgcaaacata acagccatca acgaagaggc
      361 agtcagggga acacattatc gttcgtccgt caggcctttc tttcttggca accagtattt
      421 tctgattgtc aatgaaatat tccgtgatgt caggctcgtg ggggctcccc cgtcagccat
      481 cggcaagttc gggggcgaca ctgacaactg ggtatggccc aggcatacgg gcgacttttc
      541 tcttttcagg gtttatgccg acaaggagaa taagccagct gcctattcgg aagagaatct
      601 gccctataag cctgcatacc acttccccgt gtcaattgcc ggagtgaagg agggtgattt
      661 taccatggtc ttcggatatc ccggaaggac acaggaatat gctccgtcat atcatatcag
      721 catgctgaag gatgtggtct atccgaaaac ggtggagata cgcggcagga agatatcaat
      781 catggagcag gcgatggcaa atgacccgct tatcaggctg aagtattcgg gcaagtcatt
      841 tggcattgcc aacggctgga agagatcaat cggtgaaatc cagagccttg acaaaatgaa
      901 tggtgtagca cgaaagcagg cctttgagaa ggagttcatg gagtgggtgt cagctgaccc
      961 ggaacgtctc cgcaggtacg gaaagattct cgatgagtat gctgagatct acggcagcta
     1021 tacgaccaat tattttgtaa actcttacac caatgaggtg ttcggctcaa caggagtaga
     1081 accggcatca cttgccggtg ccttcaagag agcagttgaa atggcctccg gaaaggagtc
     1141 cgggcttgac aatgaactga aaagagtaca ggagtatgcc gacgggttct tccgcaatta
     1201 tgaccgcaac gtttcggaga agctgtttgt ggctgtcatg gagctttacg gaaagaacat
     1261 tgacagtgaa tggcagactg atgtctataa ggctctggct gaatcatgca ggggagattt
     1321 cggcatggtg gccggaaagc tttttgatga gtcaatcttt accgatgaga acagggtcag
     1381 ggcattaatc ggcaactttg atcccaatgt ggttcaggag gatatcttct accggctggc
     1441 tgtctcggcg tctgacctga tcgcatcacg actcacggaa gagctgtcgg ttgccaatgc
     1501 catgctggca gagctcaaca aactgtatat ggcagctcag atggagcagg gggtagacaa
     1561 ggtcttctac cctgacgcaa actcaaccct caggcttgcc tacggaaagg tgatgggtta
     1621 tgagtcacgc gatgccgttt accatgagca tcagacaacc ctcaagggtg tgatggagaa
     1681 ggacaacccg gagatatatg attacgatgt gcctgacaga cttaaggaac tgtatgagaa
     1741 gaaggatttt ggcaggtacg gattcaacgg caatatgccg gtatgtttta ttgcaaacaa
     1801 tcacaccacg ggcggcaact cgggaagccc ggtgcttaat gccgagggtc acctgatagg
     1861 ggttaacttt gaccgtgcat gggaaggggt ggcatcagac atgatgtaca acccggagca
     1921 gagccgcaat atcagcatag atatcaggta tgcactcttc ctgattgaga agtttggcaa
     1981 tgcaggttac ctcatagatg agatgactat tgtcggagtg ccacagcaaa cgccatgatc
     2041 aggaagaaga aggcctgaag ccttctcttg agtgatacgc tgcccattcg tggaaggagc
     2101 cggatatatg taagcactca tcgtatcctg aatgcctggg cagagccggg cagtatcacg
     2161 gtaaaacagg tgccgctgcc gggagtgctc tccacggtga tggttccctt gcttaatgcc
     2221 accagctcat aaacgagctt caggcctatc ccggttccct tttcggaatc ggtgcctggc
     2281 gttgaatcaa tctcatgtcc ggtgaacagc ctcttctgac gttcgggagt gattccgacc
     2341 ccgttgtcac agatacttat ggtcacccat tcggaagact cttccctctc ctttacatgg
     2401 atcgttacct tcccgccgtg ataggaatac ttgattgcat ttgtgagaag attcctgata
     2461 attatatcaa ccagatcccg gtcaaaccag ccgtttgacc tgcccacctg ggtgaaattc
     2521 agctcaatgt tcttcctgtc agcgctgtcc ttgaggatgc tgatattggt aaggattatg
     2581 tcagcgaggt cattctcggc aggagcatac tttatcatgt cttcctggcc ccttccccag
     2641 accagcatgt tctcgaggag gctgataagt tgctgtgagt actgaatgca tgagtctgca
     2701 agtgattctg tacggtcacg gaactcctcc tccttcagca ggtagagggt gtagaggatg
     2761 tttaccaccg gacttcgcag gtcatgagca ataacagaaa agaccttgtt cttcaggctt
     2821 atcgtctcgg tgagcttttt attctgcagg gcgatctgct tattccttgc attaagctct
     2881 tccgtactgt ccgtgagctc cctggtacgc tcgtcaaccc tgctctcaag actgcggttc
     2941 agttcctcaa gttgcagcga gagcttctcc ctctctttat cacttcttac ccagtcagct
     3001 atcagcaggg ttgactgaat cagcacgaag atgaggatca ggaatgagag tatatatagc
     3061 tgctgcttct cttccagtgc atttgacagc aggatgtcag atgccactcc gaacaagatg
     3121 gccatgattg ccagcaggta catgatgtct ctccggttgc ctttcattat gcccctgatg
     3181 ctcattgaaa cggcgtaaag cagagtcagt attgccaaag cctgtatacc gaaaactgac
     3241 catgaaaaga tccttaccgg aaggaaaaca acggcagcaa tccaggggat aaaaacgatg
     3301 tccacagcca gtgaaacctt tctgaaccat ctggcgggat atatcaggca tgccagccac
     3361 atcccggagg taatcatgaa taggagtata atgtattctc cccggatgat gaggttccag
     3421 tttcttatgt caacaactgt tgcaaggtat ggggctgaga aaaacgggcg gagggcaagg
     3481 cagaggacca gtatggcaaa catcagcagt ttcctgtcgc gtctgtcaag caggtagaaa
     3541 atcaggaaga agagaaatga agctatcagg atacctgata ccagggttga gaagagccac
     3601 tggtttgaga agttgttctg tatcgagtga aatgttcctg cctttacagg cagccagaaa
     3661 cctccgcgcc ggtgttcata gttggagacc ctgatcagca gttcaagggt gtcactcttg
     3721 ggtacataac tgaagatcag gggtttatat gacgggaccg actcagccct ggttcgtgcg
     3781 ggagtgccgt tcctggccat tgtgacgcca ttgatgctta tctcatacga ggtgtcaaac
     3841 accggcatgt caaaccccat cctgtcacga tatccgcggg gcagcactat cagggcccgg
     3901 tatgtaccat agccgaaccg cggaagcttc ctgccatcaa cggtgtaatc gctccagtag
     3961 cccggaacct tggcatagca gtccgggatc agtgtgtcac aaacagttcc gggtgtgcca
     4021 tagatgaatg tgccgaaata gaactcccac tctccgttca acctcacggc gaactcatct
     4081 ccctgctcta tctcactcag gtcaagcacg cccttcccga ccctggggga gacagcagca
     4141 acactgcctg tcaggagtat cagcaggagg atagttgttg agactctttc cggcacattc
     4201 atccgggcat tcaaaggcta ttgttattta aatccccggt tttccagcag agcgtctatg
     4261 ccgggctcac ggcccctgaa ggcaatccac atctccagct cgtcctttga tcccttccgt
     4321 gagaggatct cattctcgaa gcgggctgca acctctttgt tgaagatgtc acctgtctcc
     4381 ttaaaggcct tgaaagcgtc ggcatcaagc tgctcacacc agatatagct gtagtagcct
     4441 gccgaataac cgccaatgat gtggttgaaa taggtcgacc tgtagcgggg aataatctct
     4501 ttgatcagtc cgtacttctc catagctttc ttctcaaatt ctccgatgtt cagttctaca
     4561 ggctctgtca gggagtggta ttccatatcg agtgatgagg cagccaggta ctcaacggta
     4621 ataaagccct ggttgaactt accggccttc tctatctttt ctatcagggc atcaggaatg
     4681 atttcaccgg tctggtagtg ttttgcatat accttcagca cttccggttc aaagacccag
     4741 tgttccatga cctgtgaagg cagttccacg aaatcacgtg acacaccgct ggtatactcc
     4801 gtgtttgaca gcaggttgtg cagggcatga ccaaactcat ggaagagcgt gcttgcctca
     4861 tcggtggtaa gaagtgacgg agtatcaccc gcgggaggtg tgaaatttgt gaccatgctc
     4921 accagcggcg tcacgaaatt gccattttcg tcaatacgct gtggcctcag gccgctgcac
     4981 catgcaccac cccgcttcga tgccctggga tgatagtcga tcataaggat accaacgtgc
     5041 ttgttgtcac gcttcacctc ccaggttttt acgtcaggat ggtacagtgg tatgtcattc
     5101 ctttcactga attccagtcc gtatagtctg ttggcgacat agaacattcc ctcagtaaca
     5161 ttactggccg agaaataggg tttcgtctct tcatcgctca ggtcatactt ctccttgcgt
     5221 atcttctcgg tgtagtagaa ccagtcccat ggctcgagct tgaaattgcc gccttccctg
     5281 tctatcagtg cctgctgtgc tgcagcctcg gcctttgcca ccggaagggc agctttccat
     5341 atctgtgaga ggaaattgag tactgtttca ggctccttgg ccatattgcg ctcaaggaca
     5401 taatctgcat gactctcata tcccagcagc ctggccttct caacccgcag cctgactatg
     5461 tcggcaagga tcttgttgtt gtcattttcg ttgccgttgt tgccccgcat gctgtaaccc
     5521 cggaacatct tctccctgag ggccctgttg tctgcgtagg tcataaaggg gatatagctg
     5581 ggattctgaa gggtgaagag ccatttgccc tccatctcct ttgcactggc ggctgcggca
     5641 gcctgtgccc tgatcccttc aggaatacct gagaggtcct cttcattctc tatgacgagg
     5701 gtgaaggcat tggtttctgc cagcaggttc tcgccgaatc tgacacccag cagtgacagt
     5761 tcactgttga gcttgcggag cacttcctta tcttcgggag agagacctat tccattcctg
     5821 atgaaaccaa ggtactggtc ttcaagaagc ttgctctcct cgggtgtgag attgaattta
     5881 tcacggttct catatacaga ctttaccctc tcaaagagct tttcgttgag ggatatatcg
     5941 tcactgtgct ttgagaagag cggactcatc tccctctgca gtgcctgtat cgaatcattg
     6001 gtattggcgc cggtaagcgg actccaggca cgccgtacct tttgcaacag tttgccggtg
     6061 aactgcatgg ccctgatggt gttgtcaaag gtgggc
//
LOCUS       DAOPBK010000152         5973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_11627_length_5973_cov_4.154275, whole genome shotgun sequence.
ACCESSION   DAOPBK010000152 DAOPBK010000000
VERSION     DAOPBK010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11627_length_5973_cov_4.154275"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLA17_08850"
     CDS             <1..768
                     /locus_tag="PLA17_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPK85563.1"
                     /translation="KTSDGRVMFGVPWHNRVIIGTTDVPVKEFVLEPRALDEEVDFIL
                     ETAGRYLEKKPQRSDVLCVFAGLRPLAAPRHDKDGAKTQEISRSHKMLVSPSGLVTIT
                     GGKWTTYRKMAEDTVNAGIRAKKLPFRPCRTRDLKIHGWYENPDRTNRMYIYGSDLDE
                     LMQMQKENPEWAEKLNGESDFTAGEVIWAVRKEMARTVDDVLARRVRVLYFDARLSIS
                     LAPAVAGIMARELGRDKEWIRQQVEEYTNLASGYVMS"
     gene            815..2275
                     /locus_tag="PLA17_08855"
     CDS             815..2275
                     /locus_tag="PLA17_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK85564.1"
                     /translation="MINFLKPPPYKEPLPEEKIDKTYKRLRLQVFLGAFIGYAAYYLV
                     RKNLALAIPGMIQPVENGGLGYTKLELGIALSAISIAYAFSKFIMGALSDRSDARKFL
                     VTGLILSSLLMMSVGVFPFATSAVWIIFLFMLVIGWLGGMGWPPCGRIMVHWFSHNER
                     SFKMGIWNTSHTVGSGLMGNLAAWGTMLLGGLFLVDLGGGVTVEQSWRAVFIFPSAVA
                     LLLALFCWLTLRDTPQSCGLPAIDKYRNDVPAQKNMSSDKKIPVKELFVKYIFTNKIL
                     WLIALANVFVYLIRYGIGDWAPTYCEEAGLLTSEQSKMAFSLHNYAGVPGMVLIGWVS
                     SKVFKGRCAPANVIYMVLVLLSILLYWKALPVASFLANAFGTDPDATRVTVVYSALIA
                     IGFFIYGPVALIGVQAVNLVPKNAAGTAAGFVGLFGYLLGDALLSKVLIGAVADNVSL
                     GWGATFRIFIAASILAAIFSATTWKKEKETYLGHSK"
     gene            2376..3305
                     /locus_tag="PLA17_08860"
     CDS             2376..3305
                     /locus_tag="PLA17_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HPK85565.1"
                     /translation="MKRLIVTVLASAFCLNLTVNAQTYRHISGWSQDVNDQIEGFLNS
                     TITMNIRKVAVFDSDGTTFGQVPYYLADEALYRYADEVLKERKDREAKEKLKILERMV
                     KDGNNVGKPYVENRIHFLAGLTPGEIEMIGYECYRESYSSKTYPEMKQLVANLKEYGF
                     EVYILTASPEFLYQKFVSEEYGIPVTNVVGAKCVVKDGVTTGEIIPPLPQDDGKAHTI
                     ETFIKARPLIVGGNSRGDMDMLNESAGLKIVVNPDDVTVRGPEDGPMSGYTVKAYWEK
                     EGALIVRCNDVRDPKVRFHTEEWKIRENRANPK"
     gene            3315..4499
                     /locus_tag="PLA17_08865"
     CDS             3315..4499
                     /locus_tag="PLA17_08865"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPK85566.1"
                     /translation="MKIRVLFLMAATAALLAGCAGKNTPVTGGVQEYMPDNAVIAHRG
                     TIYWAPELTESAFRWARNTGADYMELDLHRTKDSVLVIMHDRTFKRTTNVAEIFPGRE
                     DDNIGTFTWEEIMQLDNGSAFNKKNPDQARQSFAGQEVLTFEDVFRIAEGKRIKRNPD
                     GSRLFTKDETGIYHFEYEDDPADNGHRPGIYIEFKIPDEYPLIEEQVYDELTRIGWNP
                     LEGAIPSADEPFLKGNRVNVGNTRGKILVQTFSQKGMMNIKRVFREQVPASYLIGNTK
                     ANLFAQEAVTDSIVAFARECGAQFIGTNLGDENDGLSPEFAAKIRAAALKSNVYSFNT
                     VEQMEKYFGQSDGENASPLTDGMITNRTDLTVDFYHARKVRAFKTEKSPPEILADLGY
                     NQ"
     gene            4512..4841
                     /locus_tag="PLA17_08870"
     CDS             4512..4841
                     /locus_tag="PLA17_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85567.1"
                     /translation="MPAIKKLLFLLLIAAIAAFTGCKEDPPPPDEGGLKITRISISES
                     FKVPAEGTGCHYSHSGFAVSDQIVFVFVTDAGKVYTATVTSVTGAGAGFTLPIRRGKI
                     TWQVPFG"
     gene            complement(4816..5850)
                     /locus_tag="PLA17_08875"
     CDS             complement(4816..5850)
                     /locus_tag="PLA17_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK85568.1"
                     /translation="MAKPNVTLKDLARELNLSLSTVSRALQNSESISKKTRKRVLAEA
                     ERLGYFPNSMASSLRRSKSNLIGIIVPRISIYFHSRVISGIEEVAFKNGYNVVICQSN
                     DSYEREVANTQALLANRVGGVIACLALETEDNRHFLRFSKFNTPLVFFDRVCQDFACN
                     KVVIDDFEAAYKATQHLINIGCKRIAHIAGHQGTSIFKARLEGYKAALRDNDAMSSDD
                     YVKEMKNLNYNEGYNYSGSLFALNPPPDGIFCANDDSAVGIIQGAKAFGIRVPEDCAV
                     VGFSNYPVSTIVEPSLTTIDDRAIEMGQAAAKLLLQQIEDKSDFVSSQTIVIETDLII
                     RESTQRAPAR"
BASE COUNT         1605 a         1476 c         1494 g         1398 t
ORIGIN      
        1 aagacaagtg acggaagggt aatgtttggt gtgccatggc acaaccgcgt tatcatcggc
       61 accactgatg ttcccgtgaa ggagttcgta cttgaaccca gggccctcga cgaggaggtt
      121 gactttatcc ttgagacagc gggcaggtat cttgagaaaa aacctcagcg cagtgacgtg
      181 ctgtgcgtct tcgccggcct gcgaccgctg gcggcaccca ggcatgacaa agacggagcc
      241 aaaacacagg agatatcgcg aagccataag atgcttgtat caccttcagg cctggttacc
      301 atcaccggcg gcaaatggac cacctaccgc aagatggcgg aagatacggt taatgccggt
      361 atcagggcaa agaagcttcc gttcagaccg tgccgcacca gggaccttaa gatccacggc
      421 tggtatgaaa atcccgacag aacaaacagg atgtacatct acggcagcga ccttgatgaa
      481 ctgatgcaga tgcagaagga gaatcccgaa tgggctgaaa agcttaacgg agagtcagac
      541 ttcactgccg gcgaggttat atgggccgta agaaaagaga tggcgcgcac agttgatgac
      601 gtgctggccc gccgggtaag ggtcctctac tttgatgcaa ggctgtcgat atccctcgct
      661 ccggcggtgg ccggaatcat ggcccgggaa ctgggacgcg acaaggagtg gatcaggcag
      721 caggttgagg aatacacgaa tctggcatca gggtatgtga tgtcatgata tctgacagaa
      781 aacctgacaa agcaacctga caaacaactc accgatgata aactttttaa aaccaccccc
      841 atacaaggaa cccctgcctg aggagaagat tgacaaaact tacaagcggc ttcgtctgca
      901 ggtctttttg ggagctttta ttggctatgc agcatattac cttgtcagga agaatctggc
      961 tctggccatt cccgggatga tccagccagt tgagaacggc ggtctgggtt acaccaagct
     1021 ggagctcgga atagccctct cggccatatc aattgcctat gctttcagca aattcatcat
     1081 gggagctctt tctgacaggt cagatgccag gaagttcctc gtaacgggtc tcatcctctc
     1141 ttcactcctg atgatgtcag tcggggtttt tcccttcgcc accagcgcag tatggataat
     1201 tttcctcttc atgcttgtca taggctggct tggcggtatg ggctggccac catgtggccg
     1261 cataatggtt cactggttct cgcacaatga gcgaagcttc aagatgggta tatggaacac
     1321 ctcccacact gttggcagcg gcctgatggg taacctggca gcctggggca ccatgctcct
     1381 tggcggcctc tttctggttg acctcggagg aggagtaacc gttgagcaga gctggagggc
     1441 agtcttcatc ttccccagtg cagtggcact gctgctggcg ctcttctgct ggctgacact
     1501 gcgtgacaca cctcagtcat gcggccttcc cgccatagac aaatacagaa atgatgtgcc
     1561 ggcacagaag aatatgagct ccgacaagaa gatcccggtg aaggagctct ttgttaaata
     1621 catcttcacc aacaaaattc tgtggcttat tgctcttgcc aacgtcttcg tctacctcat
     1681 ccgttacggc ataggcgact gggcacccac atactgtgag gaagcagggc tgcttaccag
     1741 cgaacagagc aagatggcct tttcactgca taactatgcc ggtgtaccgg gaatggtgct
     1801 catcggatgg gtctcctcaa aggtgttcaa gggtcgttgt gctccggcaa acgtcatcta
     1861 tatggtactg gtgctgctca gcattctgct ctactggaag gccctgcccg tggcttcatt
     1921 tcttgcaaat gcctttggca ctgatcctga tgccacaagg gtcacggtgg tctattctgc
     1981 cctgatagcc atcggattct tcatctatgg cccggtggcc ctcatcggag tgcaggccgt
     2041 caacctcgta ccgaagaacg ctgcagggac tgctgccggc ttcgtaggcc tcttcggata
     2101 cctcctgggc gatgccctcc tctcgaaggt gctgataggc gcagtggccg acaacgtaag
     2161 ccttggctgg ggtgcaacct tccggatctt cattgctgcc agcatactgg ctgccatctt
     2221 ctcggcaaca acatggaaaa aggaaaagga gacatatctc ggacactcaa aatgattaac
     2281 atgaaaccca catgtatcaa acagtcaaac acctgtttat atgattctca gtcatatggg
     2341 ttccataata cctttaaaat caaaatttta ttataatgaa acgactgata gtaactgttc
     2401 ttgcatcagc attctgcctt aacctgacgg ttaatgcaca gacctaccgg catatttccg
     2461 gctggtcaca ggatgtcaat gaccagattg aagggttcct caactcaacc atcaccatga
     2521 acatcaggaa agtggcggtg tttgacagtg acgggactac cttcggccag gtgccttatt
     2581 accttgctga cgaagcactc taccgttatg ctgacgaggt tctgaaggag agaaaggacc
     2641 gtgaggcaaa ggagaaactg aaaatcctgg aaagaatggt caaagacggc aacaacgtag
     2701 ggaaacccta cgtggaaaac aggattcatt tcctggcagg tctgacaccc ggagagatag
     2761 aaatgatagg atatgaatgc taccgggaat catacagcag taaaacctac ccggagatga
     2821 aacagctggt tgcaaacctg aaggaatatg gtttcgaggt ctacatactg acagcatcgc
     2881 ctgagtttct ctaccagaag ttcgtgtcag aggaatatgg cattcctgtc accaacgttg
     2941 tgggcgccaa gtgcgttgta aaggacggtg tcaccaccgg cgagatcatc cctcccctcc
     3001 cgcaggatga cggtaaggct cataccatag agacctttat caaggcaagg cccctgattg
     3061 taggaggcaa cagccgcggc gacatggata tgctgaacga gtcggcagga ctgaagattg
     3121 ttgtcaatcc ggatgatgtt accgttcgcg gcccggagga cggccccatg agcggctata
     3181 cggtgaaagc atactgggag aaggaagggg cactgattgt caggtgcaat gatgtgcgtg
     3241 accctaaggt cagattccac actgaagagt ggaagatcag agaaaacagg gcaaatccta
     3301 aatgatcagt cacaatgaaa ataagagtac ttttcttaat ggccgctact gctgccctgc
     3361 tggcaggatg tgccggcaaa aacactcccg taacgggagg agtgcaggaa tacatgcctg
     3421 acaacgcggt catagcgcat cgcggaacca tatactgggc tcccgagctt accgaatcag
     3481 ccttccgctg ggcacgcaac accggcgctg attacatgga actcgatctg caccggacaa
     3541 aggacagtgt actggtaata atgcatgaca gaacctttaa acgtaccacc aacgtggcgg
     3601 agatctttcc cggcagggaa gatgataaca taggcacctt cacctgggaa gagattatgc
     3661 agctcgataa cgggagtgct ttcaacaaaa agaaccctga ccaggcccgg cagagcttcg
     3721 caggtcagga ggtactgaca tttgaagacg ttttccgcat cgccgaaggc aaacggataa
     3781 aaagaaatcc tgatggcagc cgcctcttta caaaagatga aaccggaata taccactttg
     3841 agtatgagga tgatccggca gacaacggtc acaggcccgg aatatatatt gaattcaaaa
     3901 tacctgatga ataccctctc atcgaagaac aggtttacga tgaactgacc aggattggat
     3961 ggaatccgct tgagggtgct ataccgtcag ctgatgaacc attcctgaag ggaaacaggg
     4021 tcaacgtcgg caacaccagg ggaaaaatcc ttgtgcagac attttcacag aagggaatga
     4081 tgaacatcaa aagggtcttt cgcgaacagg taccggcaag ctatctcatc ggcaatacaa
     4141 aagccaacct ctttgcacag gaggctgtga ctgacagcat agtggctttt gcacgtgagt
     4201 gcggcgcaca gtttataggc accaatctcg gggatgagaa tgacggcctc tcgccggagt
     4261 ttgctgcaaa gatccgtgcc gcagctctta aatcaaacgt atactcattc aatacagttg
     4321 agcagatgga gaaatatttc ggccagagtg acggggagaa tgcctcccct ctcactgacg
     4381 gcatgatcac caaccgcact gacctgacag tagatttcta tcatgcacgg aaggttcgtg
     4441 cctttaaaac tgaaaagagc ccgccggaga tacttgctga cctggggtat aatcagtaaa
     4501 atgagaattg catgcctgcc ataaagaaac tgttatttct gcttttgatt gcggcaatag
     4561 ccgccttcac cgggtgcaag gaagatccgc ctcctccgga tgagggagga ttgaagatca
     4621 caaggatatc catttctgaa agctttaaag ttcctgccga aggcacaggt tgccattaca
     4681 gtcacagcgg atttgcagta agtgaccaga tagtgttcgt gttcgttacc gatgccggaa
     4741 aggtctatac cgccacggta acctcagtga caggagcagg agccggcttt acccttccaa
     4801 tccgcagggg aaaaatcacc tggcaggtgc cctttgggta gattcacgaa taataaggtc
     4861 tgtctcgatg actattgtct gagatgatac aaagtcactc ttgtcttcaa tctgctgaag
     4921 taagagcttc gcagctgcct gccccatctc aatggctctg tcgtcaatag ttgtcagtga
     4981 tggttcaaca atagttgaaa ccggataatt actaaacccg accactgcac aatcctcagg
     5041 tactcttatt ccaaatgcct tggcgccctg aatgattcca acagcagaat catcattggc
     5101 acagaagata ccgtcaggtg gcgggttaag ggcaaagaga ctgcctgaat aattataacc
     5161 ctcattataa ttaagatttt tcatctcttt cacatagtca tcagaagaca ttgcatcgtt
     5221 atctctcagc gctgccttgt atccttcaag tcgtgccttg aaaatacttg ttccctgatg
     5281 acctgcaatg tgggcgatac gtttgcagcc tatatttata aggtgttgag ttgctttata
     5341 ggctgcttca aaatcatcaa taaccacctt gttacaggca aaatcctggc ataccctgtc
     5401 aaaaaacacc aacggggtat tgaatttgga aaaccgcaag aaatgccggt tgtcttctgt
     5461 ttcaagtgcc agacaggcaa taactccccc gactctgttc gccaataatg cctgagtatt
     5521 ggcaacctca cgctcatatg aatcattcga ctggcagatg acaacattat agccattctt
     5581 aaacgccacc tcctcaatac cggagattac ccttgaatga aagtaaatac ttatccgtgg
     5641 aacaattatt ccaatcagat ttgatttgct gcgtctcagg cttgatgcca ttgaattcgg
     5701 gaaataccca agccgctcag cctctgcaag gactcttttt ctggtctttt tactgatcga
     5761 ttcactattc tgcaaagctc tcgacacggt ggagagtgac aggttcaact ctcttgccag
     5821 atcttttagt gttacattcg gtttagccat tatcagcctt cacaaacgtt tgaataaatc
     5881 aattctattt gaggtgcaaa tataacaata tatttatcaa atcatccttg aaatcaggca
     5941 ggaaaattgc cctgccggca gagccctgcc cgg
//
LOCUS       DAOPBK010000153         5940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_11682_length_5940_cov_5.606627, whole genome shotgun sequence.
ACCESSION   DAOPBK010000153 DAOPBK010000000
VERSION     DAOPBK010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11682_length_5940_cov_5.606627"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..2385)
                     /locus_tag="PLA17_08880"
     CDS             complement(316..2385)
                     /locus_tag="PLA17_08880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPK85569.1"
                     /translation="MMKKLSIITLLLVIALMGGCKTEKREKAVTPVIDVSLTDAEKSG
                     GVLTPEIMWKYGRVGGIALSPDGSTVLFQVTNYDLLTEARATNIWSVPASGGDPVQLT
                     TEGGSGAQWIENGARIAFIAGGKVMAMNPDGTGKKEITGLNDFEILSFSPAGDKVSFT
                     RRVKLDQTANEKYGLSNAKVRIIDDLMYRHWDYWHDYSYSHLFTASFDGAAISNEKDI
                     MEGQRFDAPDAPWFDEADIAWSPDGKYIAYACKKLTGKAYSLSTNTDIYLYDVTTGEE
                     INITEGNMGYDKTPLFSPDGTMIAYSSMEEGGYESDLERLFVYNIASGERKWVSQGWD
                     YNVASIQWNGTGEIWFTSPYLGRQPVFRISMADGAVTKVTEGDYDMGSLQLRNNTLVA
                     GIQSMARPTEVAKVDMADGQFSMITDVNGHIYESIKMGQSEERYIKTKDGKDLQLWII
                     YPPDFDPAKKYPALLYCKGGPQGPLGQSWSYRWNYQMMAANGYIVVAVNRRGNSGFGS
                     YWREQISGDYGGKNMQDYLDGIDAMAKEPFVDKDRLGAVGASYGGYSVFYLAGMHNKR
                     FKAFVSHCGVYNTVSEYGSTEEYWYPNKDYQGTPWDKPQPPGYNQFSPHTMIDKWDTP
                     ILIITGANDFRIPYTQSMEAFNSAILRGVPARFLFFENESHWVTKPQNSVIWQKEFFE
                     FLDSYLK"
     gene            2682..5765
                     /locus_tag="PLA17_08885"
     CDS             2682..5765
                     /locus_tag="PLA17_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85570.1"
                     /translation="MSSFKMNTITVRFLRLSFALLTVWLIAGVNSDAQYFGRNKPGYS
                     SFRFDVLQTPAFEIYHYMKNDSLLKTISGWSEEWFSIHQAIFRDTFARKNPLIFYNNH
                     ADFQQTNAISGLIGTGTGGVTEGMKNRVIMPIALTLAQTDHTLGHELVHAFQFNMFLR
                     TDSTQKYSMNNIPLWMIEGMAEYFSLGSVDPNTAMWMRDAVLNKDFPTLKQLSSDSRY
                     FPYRYGQAFWALVGKTWGDTTIMSLFRKTAQYGFSKAADTILGMDEKTLSGAWASAME
                     TYYRPFLKADADELAGKQLITTENGGRINISPAISPDGKYVAFFSEKNIFTLDLFFAD
                     ASTGKIISRLSGAAARAYDDFNYIESGGSWSPDSKKYVFVLFSDGINKLAFVDAKRGR
                     ITREVALSGVPAISNPSWSPDSKKIVFTGSSEGISDLYLYDIMSGDIEKLTDDFTSNI
                     HPSWSSDSRTIVFSQEKMNESGHRKFSYSIAMLDLNTRKVSEIDVFDGAYNMNPLFSA
                     DDSHIYFLSDADGFRNMYKYEIATGKLLRLTSYMTGITGITPWSPAISLSRDRNRVVY
                     NYYIKNSYHILVADENEFSPEEADGSVINFDAGTLPPLEHLSVNLIDSTLYARKEAVQ
                     VPADMVKELPYKPKFKLDYISNNASMGASSGIYRNNLGGSVIMIFSDMIGDNQLYSAL
                     SLNGEIYDFAGQAAYIRQKGKIKLGTAVSHIPYRAGSMTFGRDSVKLQGEMVPVDVIQ
                     LDYIRMFEDNITLYASLPFSQVRRIEATASASWYYYRIDRFNYYYLLNGYSIGGTREK
                     MPAPDGSNYQQLSFAYVKDNSLFGMTAPMHGGRARLQIDKYFGATQIFTGLIDYRQYI
                     YRKPFTFAFRLYNYGMYGKVTGENILQPLYLGYPWLIRGYENISYNTGNTPELTSLNI
                     SRLSGTRMAVANAEIRFPFSGPERLALIKSKWFLTDLNLFFDAGLAWNRGDKIVFNDW
                     ESHTADNEKFPLYSTGVSLRINFFGYLILEPYYAFPFQNGGFKNGVFGLNFLPGW"
BASE COUNT         1494 a         1379 c         1471 g         1596 t
ORIGIN      
        1 ggctttccca atttttgtgt tgggaaagca acctctcagt tagcgtttac acaatttaat
       61 ctatactcca agggcgcacc cgtaaaggtt attggcatat tgcgaatagc catattctgt
      121 ctgccacacc acagatagtc ttcgtctggc tggatacgtt ttctttacgg actgctacaa
      181 taaggttaga gttgtatttg agggaaccgc cgtatacgag accaatacgt acggtggtgt
      241 gagaggtcgg aaaatgaaac aggaggaaac ctgtttcatt ttcctcctac ttgattgctc
      301 tattctgcct cagtcttact tcagatagct gtcaaggaat tcaaagaatt ccttctgcca
      361 gatgacagag ttctgtggtt tggtaaccca gtgactctcg ttctcgaaga acaggaaccg
      421 tgccggcacg ccgcgcagga tggctgagtt aaatgcttcc atcgactggg tgtaaggtat
      481 ccggaaatca tttgctccgg tgatgatcag gatgggtgtg tcccatttgt caatcatcgt
      541 atgaggtgag aactgattgt atcccggagg ctgaggcttg tcccacggtg tgccctggta
      601 atccttgttg gggtaccagt actcctcggt ggagccgtac tcggaaacag tgttataaac
      661 accgcaatgc gacacgaacg ccttgaacct cttgttgtgc atcccggcaa ggtagaagac
      721 tgagtagccg ccatagctgg ctcccacggc accaaggcgg tctttgtcga cgaacggttc
      781 ctttgccatc gcgtcaatac cgtcgaggta gtcctgcatg tttttaccgc catagtcgcc
      841 cgagatctgc tcacgccagt agctgccgaa gccggaattg ccgcgacggt tgacagccac
      901 cacgatatag ccgtttgcgg ccatcatctg gtagttccag cggtagctcc agctctgtcc
      961 gagcggtccc tgcggcccgc ccttacagta caacagtgcc ggatatttct ttgcggggtc
     1021 aaagtcaggc ggatagatga tccacaactg aagatccttg ccatccttcg tcttgatata
     1081 gcgctcctcg ctttgaccca tcttaatact ctcataaata tgaccgttga cgtcggtgat
     1141 catcgagaac tgcccgtcag ccatgtcaac ttttgccacc tctgtcgggc gtgccattga
     1201 ctgaatacct gcaacaagag tattgttcct gagctgcagt gaccccatgt cgtaatcacc
     1261 ttcggtcacc ttcgtgacag cgccgtcagc cattgaaatc ctgaataccg gctgcctgcc
     1321 gaggtagggg ctggtgaacc atatctcacc ggtgccgttc cactggatgc tggcaacgtt
     1381 atagtcccat ccctgtgaaa cccatttccg ttcgccggaa gctatattgt agacaaacag
     1441 ccgctcgagg tccgactcat agccaccttc ctccatgctg gagtatgcta tcatggtacc
     1501 gtcgggagag aagagaggag tcttgtcgta acccatgttt ccttcagtaa tgtttatctc
     1561 ctcaccggtt gtcacgtcat acaggtagat atccgtattg gtgctgaggg agtatgcttt
     1621 gccagtcagt tttttgcagg cgtacgcgat atacttcccg tcaggactcc atgcaatgtc
     1681 agcctcgtca aaccacgggg catcgggggc atcaaaacgc tgtccctcca tgatatcctt
     1741 ttcgttgctg atggcggctc catcaaacga ggcggtgaag agatggctgt atgagtagtc
     1801 atgccagtag tcccagtgcc tgtacatcag atcatcaatg atacgcacct tagcattgga
     1861 gagaccatat ttctcgtttg ccgtctggtc aagcttgaca cgacgggtaa aggaaacctt
     1921 gtctccggcg ggggagaatg agagtatctc gaaatcattc aggccggtaa tctctttctt
     1981 tcccgtaccg tcaggattca tggccatgac tttgcctccg gcgatgaagg cgattctggc
     2041 tccgttctct atccactgcg ctcctgatcc tccttcggtg gtcagctgaa caggatcacc
     2101 tcccgatgcg ggaactgacc agatgttcgt ggcacgtgct tcggtcagaa ggtcatagtt
     2161 tgtcacctgg aagaggactg ttgatccgtc gggcgagagc gcaattcccc caacccggcc
     2221 atatttccac atgatctccg gggtaagtac acctccgctt ttttcggcgt cagtaagtga
     2281 gacatctatg accggtgtca cggctttttc tctcttttcc gttttgcatc cacccatcag
     2341 ggctatgaca agcaaaaggg tgataatgga tagttttttc atcatagtat gattttgatt
     2401 tttagggtat gatggaaccc cccatgattg agaattctat aaacaggttt tttgcactca
     2461 atacatgtgg gattcatatt gttctggttt ctgctgccgg ttaaaatact actgttgtca
     2521 aaaataaaaa attaatactg caatgcctca taaacagact taaaaccatc cacggggaat
     2581 ggcatattat ttgaacaatt aattgcagac aggttagttt aattaattga aatgcatgga
     2641 ttaacttttg gagtattcgg gtgataaaac tttgacaggc tatgagttcg ttcaaaatga
     2701 atacgataac ggtcaggttc ctcaggcttt catttgctct gttgacagta tggctgattg
     2761 ccggggtgaa ttctgatgcc cagtatttcg gaaggaataa accgggatac agttctttca
     2821 gattcgatgt tttgcagacg ccggctttcg agatctatca ttacatgaag aatgattcat
     2881 tgctgaaaac gatatcagga tggtcagagg agtggttcag cattcatcag gcaattttca
     2941 gggatacgtt cgccaggaag aatccactga tattttataa taatcacgct gattttcagc
     3001 agaccaatgc tatcagcggc cttatcggaa caggtacggg aggtgttact gaaggaatga
     3061 agaacagggt catcatgccc atagctctga ccctggcaca gactgatcac accctgggcc
     3121 atgagcttgt gcatgcattc cagttcaata tgttcctgcg gacagactcc acccagaagt
     3181 attcaatgaa caacatcccg ttatggatga ttgaagggat ggccgaatat ttctcgctgg
     3241 gaagtgttga tcccaacacg gcaatgtgga tgagggatgc agttttgaat aaggatttcc
     3301 cgacgctgaa gcagctgtct tctgactcca ggtacttccc ctacaggtat ggccaggctt
     3361 tctgggccct ggtggggaaa acgtggggtg atacaaccat tatgtccctg ttcaggaaga
     3421 cagcacaata tggattttca aaggcggctg acaccatcct cggaatggat gagaaaaccc
     3481 tttcaggagc atgggcaagt gcaatggaga cctattacag gccattcctg aaggctgatg
     3541 ctgacgaact tgcagggaag cagctgataa caactgaaaa cggcggcagg ataaatatct
     3601 cgcccgcaat aagccctgac ggaaaatatg tcgcattctt ttcagagaaa aatatattca
     3661 ctcttgacct tttctttgcc gatgcttcaa ccgggaagat catcagcagg ctatcaggag
     3721 cagcagcacg tgcctatgat gattttaact atatcgaatc cggcggaagc tggtcgcctg
     3781 atagtaaaaa gtatgtgttt gtcctcttca gcgacggcat taacaagctg gcgtttgttg
     3841 acgcaaagag gggcaggatt accagggaag tagccctgag tggtgtgccg gcaatttcga
     3901 atccatcatg gtcaccggat agtaaaaaga tagtctttac aggatcttcg gaagggataa
     3961 gtgacctgta cctctatgat atcatgtcag gagacattga aaaactgacc gatgatttta
     4021 catcgaacat acatccttca tggtcatcag acagccggac aatagttttc tcgcaggaaa
     4081 aaatgaatga atccggtcac cggaagtttt cctattccat cgcgatgctt gaccttaata
     4141 caagaaaagt cagtgagata gatgtttttg acggcgccta taatatgaat cctctctttt
     4201 ctgctgatga ctcgcatatt tatttcctct cggatgctga cggattcagg aatatgtaca
     4261 aatatgaaat tgcaacgggt aagctcctga ggcttacctc gtacatgaca ggcataacag
     4321 ggataacacc ctggtcaccg gcaatcagct tgtctcgtga caggaacagg gtagtgtaca
     4381 attattacat taaaaactca tatcatattc tggttgctga tgaaaatgaa ttcagtcctg
     4441 aagaagcgga cggatcagtg atcaactttg atgcaggtac cctgccgcct ttggagcatc
     4501 tgtcagttaa cctcatagat tcaacgctgt atgccaggaa ggaggctgta caggtgccgg
     4561 ctgacatggt gaaggagttg ccgtataagc ccaaattcaa gctcgattat atctccaaca
     4621 atgccagcat gggagcttca tcagggattt acagaaacaa cctgggtggg agtgtcatta
     4681 tgatcttcag tgatatgatt ggtgacaacc agctctattc ggccctgtct ctgaacggtg
     4741 aaatatatga ttttgcaggg caggcagcct atatcaggca gaagggcaag ataaagctgg
     4801 gtacagctgt ctctcatatc ccttaccgtg ccggctcaat gacgtttgga agagattcag
     4861 tcaagctgca gggtgagatg gttcctgtag atgttattca gcttgactat atccgcatgt
     4921 ttgaggataa tatcactctg tatgcttccc tgcccttctc ccaggtgagg aggattgagg
     4981 caacggcctc ggcatcatgg tattattatc gcattgacag gtttaattac tactatctgc
     5041 tgaacggtta cagtataggt ggaaccaggg agaagatgcc tgctccggat ggcagcaatt
     5101 accagcagct ttcattcgcc tatgtcaaag acaactcact gttcggtatg actgccccga
     5161 tgcatggtgg cagggccagg ctccagatag acaagtactt cggggccacg caaatcttta
     5221 caggtcttat agactacagg cagtacattt atcgcaaacc atttactttt gccttccggc
     5281 tgtataatta cggcatgtac gggaaggtga ccggggagaa tatactgcag cctttatatc
     5341 tcggttatcc ctggttaata agaggatatg agaatatctc gtataatacc gggaatacac
     5401 cggagctgac atcgctgaat atctcgcgcc tgagtggaac caggatggct gtggcaaatg
     5461 cggaaataag gtttccgttc agcggtcccg aaaggcttgc cctgataaag tcaaagtggt
     5521 ttctgaccga cctcaacctc ttcttcgatg ccggattggc gtggaacagg ggtgacaaga
     5581 tcgttttcaa cgactgggag tctcatacag ctgataatga gaaatttcct ctttacagta
     5641 ccggtgtatc actaaggata aactttttcg gatacctcat tcttgagcct tactatgcct
     5701 tcccgtttca gaacggggga tttaaaaacg gagttttcgg gctgaacttt ttgcccggct
     5761 ggtagaggat accgggagtt ggtttcccac cggttagtca aggataccgg gagttgattt
     5821 cccgccaatt ggtagaggat ttcgggagtt gattttcctg attacgaaaa caattcaaaa
     5881 gccactgatg tcttcagggg ttctgtggtt ctgtcaggtg cgtgaccagc agcatcaccg
//
LOCUS       DAOPBK010000154         5909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_11743_length_5909_cov_4.194055, whole genome shotgun sequence.
ACCESSION   DAOPBK010000154 DAOPBK010000000
VERSION     DAOPBK010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11743_length_5909_cov_4.194055"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLA17_08890"
     CDS             complement(<1..780)
                     /locus_tag="PLA17_08890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPK85571.1"
                     /translation="MEREDFLKDLKDKKHQWDFIIIGGGATGLGVAADAVTRGYKTLL
                     LEQSDFAKGTSSRSTKLVHGGVRYLQKGDVALVREALRERGYLLRNAPHLVKNQRFII
                     SNYRWWEKPFYTIGLTFYDLLSGRMSLGRSLPMSRSSVIKSIPQIVRKGLKGGVVYHD
                     GQFDDSRLAVNLMQTAIEKGATVVNYTKVIQLLKSPDGQVSGVVAVDELDGTQFTAEG
                     KCIVNATGIFVDSIIRMDNPEEKPMVRPSQGVHLVVERSFLG"
     gene            1245..4031
                     /locus_tag="PLA17_08895"
     CDS             1245..4031
                     /locus_tag="PLA17_08895"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPK85572.1"
                     /translation="MTGNFSFKVLAAVMTIAIAGCATSKKTVTGDPSGRTPGAEREFR
                     AAWVATVANINWPSRPGLSVEEQKSEAIALLDLLYKNNFNAVIFQVRPHCDAMYPSDI
                     EPWSYYLTGEEGKAPDPYYDPLQFWIDEAHARGIELHAWLNPYRAHSPAGGPLTDVSI
                     VRKRPDLVLKLEVENYWWMDPALKGTQDHSYNVVMDLVRRYDLDGIHFDDYFYPYPDY
                     NNYKDFPDDQSWQAYQASGGKLSRSDWRREAVNTFIERLYKGIKAEKPWVRFGLSPFG
                     IWQPYNPPAIGSGFNQHETLYADAKLWLNKGWIDYYSPQLYWPINQIAQSFPVLLGWW
                     KDENLKGRHLWPGINIGLSPASRAADETINQIMVTRGLLPGSPGVIHWSIGPLVRTPG
                     LVRAVADGPYRRPALVPPMPWLDRKAPAPPVVSRKAENGTLKLTWTHPDPADIGRWVV
                     YYKYGTQWNQHIHGSATTEDSLPAFTLNRTYLARTSRDKVTGADQAFTALDSVAVSAV
                     DRFGNESIIITMGVNEFTLADAPDPEKSLAEFYDGMKQPPVPVPAVTPGINVLLDEYP
                     DLIMGKRVGLITNPSAVGIDMRSTVDILAATPGVNLVALFGAEHGVRGAQHGRIFTDG
                     EKDPVTGIPVYSLYGESWAPKRECLDSIDVMLFDIQGVGSAWYTYKFSMSHAMEACAK
                     AGVPFIVLDRPNPLGGRIVEGPMHDTISIYRHRLPLRHGMTYGELAKMWNETEGYGAD
                     LTVIRMKGWNRSMMWSETGLQWVMPSPNMDNWETAVVYPGQCLFERTNMSEGRGMTKP
                     FLVTGAPWVNAEQAAADLNARGIAGAYFRPLYFIPRSSGPVITRTSKPWNEMCGGVEI
                     ILTDPAAYRSVEASLHIIDAYRKTSPDSLVWNPPTLIRRLNEPGVTVEEVVKACQDDI
                     REFMETRQKYLLYR"
     gene            4028..>5909
                     /locus_tag="PLA17_08900"
     CDS             4028..>5909
                     /locus_tag="PLA17_08900"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPK85573.1"
                     /translation="MTKNLFVIALATMLLIGCGTTGRTGKSGVEDPAATAENAVAIAA
                     RPTAENTAEIAARAAETLAAIPCRCDSIGNNPPMVKFLRLPEPLTHQPQFDILCKSEY
                     PATVSINGQVVKQYKTGIFFKTVTFDEGVNRITAEAVWPDGRKAVCEHVVIYEKRDLS
                     RQPFPLWIEKRSFEPSADMEVLPGEVVKVSFRASKGQEAWLDIKPGRKRIKCLREDYN
                     DYSLYRAEVPVRDFAAGVPHTFAVRLVPAAGGAPEGVFRPDLSRRVTVRNPEDFPLVR
                     VKNDYSRLVYNLGAPRLGGPIRSELGPGVILKTSGKIGSNYRIRLSNTETGFISEDDV
                     EIQGGSKMQPLYNVTSMSCGPSARGDVVTIPYLVPVPYEVRPDPDSKRLVVTLFGVES
                     AATWVTHRTGCRVVDKLTWEQSTPETFRVYVNLKSEKIWGYDIRPAGRSLILTIRYAP
                     VFDPAGEKPLAGLKIAIEAGHGGSNSGAIGLSGLVEKEINLDLSFRLGELCDSMGAEV
                     LQVRESDVDMTLPDKRDKAIKAGADMLISIHANAGGSGYLSVAGTSTYWHNPFWAPLA
                     KTIYDRMLETGLAEFGVVGSFNYTVTRTSQMPAVLVEQAFMSHAGDEEKLADPEFRQL
                     MAV"
BASE COUNT         1416 a         1509 c         1647 g         1337 t
ORIGIN      
        1 cccgaggaaa ctcctttcaa cgacaaggtg aacaccctgg ctcggtctca ccataggctt
       61 ctcttccggg ttgtccatcc tgattatgct gtcgacaaag atgccggtgg cattgacaat
      121 gcatttcccc tcggcggtga attgggtgcc gtcaagctca tccacggcca ccactcctga
      181 aacctggccg tcagggctct tgagcaactg tatcaccttt gtataattga caactgttgc
      241 ccctttttcg atggctgtct gcatcaggtt gactgccagc cgggagtcat caaactggcc
      301 gtcatgataa acgacacctc ccttgagtcc tttacggaca atctgcggga tggatttgat
      361 gacactgctg cggctcatgg gcagggagcg tcccaggctc atcctgcccg agagcaagtc
      421 gtaaaaggtg agcccgatag tgtaaaaggg tttctcccac catctgtagt tactgattat
      481 aaaacgctgg ttcttaacca ggtgtggggc attcctgagg aggtaacccc tctcgcgaag
      541 tgcttccctg accagggcta catctccctt ctgaaggtag cgaacacctc cgtgaacaag
      601 cttggtgctg cgcgacgatg tgccctttgc aaagtcactc tgttcaagca gcagggtctt
      661 gtaacccctg gtgacagcat ctgctgccac accaagaccg gtagcacctc ctccgatgat
      721 aatgaaatcc cactgatgct ttttgtcttt cagatccttt aaaaaatctt ctctttccaa
      781 tgttatagtg gcttaaatat gattattttc agaaaacatg tcaaaactat gacattgatt
      841 tcttaaatca aagcagaata ttaagcaaca gagcgcaatg atttggtcat ttatgttctg
      901 tgattctgag aaatctggcg tggcaccata atataatagt ggcggaacct gagaagaacg
      961 gttatatgag attgcatgat ctcagcttac gcctggtggt tcccgattga acacctataa
     1021 tgctttaagc cctacaggat atcctggtca tgcttctgac tgcggactgc tgctgcctgc
     1081 tgaatttgta taatctccca ttatttttta tcaggctgtc tgtcagccgg agaggttttt
     1141 tgtgtttgta tctgtgtccc ggtgaacctg atttaattgc aatatcaata cctttaggag
     1201 gatttttata tttgtatcag tacaccttta aaacctcaac ctctatgaca gggaatttct
     1261 cattcaaagt acttgcagcg gtgatgacaa ttgccattgc cggatgtgca acctcgaaga
     1321 agaccgtaac aggtgatcct tcaggccgta caccaggcgc cgagcgggaa tttcgagcag
     1381 cctgggtagc caccgttgcc aatatcaact ggcccagcag accaggcctc tcagttgagg
     1441 aacagaagag cgaggctata gctctccttg acctgcttta caaaaacaat ttcaacgcgg
     1501 tcatcttcca ggtcaggcca cattgtgacg ccatgtaccc cagcgatatc gagccgtggt
     1561 catactacct gaccggtgag gagggcaaag cccctgatcc ttactatgac cctctgcagt
     1621 tctggataga tgaggctcat gcaaggggca ttgaactcca cgcgtggctc aacccttacc
     1681 gtgcacacag cccggccggc ggaccgttaa cagatgtctc aattgtcaga aaacgccctg
     1741 acctggtgct taagctcgag gtggagaact actggtggat ggacccggcc ctcaagggca
     1801 cgcaggatca ctcatacaat gtggtgatgg atctggtacg cagatatgat ctcgacggca
     1861 tccatttcga cgattacttc tatccgtacc ccgactacaa caactataag gatttccctg
     1921 atgaccagag ctggcaggcc tatcaggcct caggcgggaa gctttcgcgc tctgactggc
     1981 gcagggaggc tgtaaatacc ttcatcgaac ggctctataa gggcatcaag gccgagaagc
     2041 catgggtcag gttcgggctc agtcccttcg gcatctggca gccttacaat cctcctgcca
     2101 tcggcagtgg ctttaaccag cacgagacac tttacgctga tgcgaagcta tggcttaaca
     2161 agggatggat tgactattac tcgcctcagc tttactggcc aatcaaccag attgcccaga
     2221 gttttccggt gctcctcgga tggtggaagg atgagaacct caaggggcgt catctgtggc
     2281 cggggatcaa cattgggctg tcgcctgcat cgagggctgc cgacgagacc ataaaccaga
     2341 tcatggtcac gcggggtctc ctgcccggaa gtccgggcgt gattcactgg agcatcgggc
     2401 cactggtcag aacgccgggg ctggtcaggg ctgtcgctga cgggccgtac cgcaggcctg
     2461 cactggtgcc tccgatgcca tggctcgaca ggaaagcccc ggcacctccc gtggtaagca
     2521 ggaaggctga aaacggtacc ctgaagttga catggaccca tcctgatcct gctgacatag
     2581 gacgctgggt ggtatactac aaatacggta cgcaatggaa ccagcatatc catggcagcg
     2641 ccacaactga agattccctg ccggctttca cacttaaccg cacatatctg gcccgcacct
     2701 cccgtgacaa ggtaacaggg gcagatcagg ctttcactgc tcttgactct gtagccgttt
     2761 cagcagttga ccgtttcggg aacgaaagca tcattatcac gatgggagtc aatgaattca
     2821 cccttgccga tgcgcctgat cctgaaaaaa gcctggcaga gttctatgac gggatgaaac
     2881 agccaccggt acctgtgccg gcagtgactc cgggaataaa tgtacttttg gacgaatatc
     2941 ccgacctgat catgggcaaa agggtgggtc tcatcaccaa cccgtccgcg gttggtattg
     3001 atatgcgaag cacagttgac atacttgccg ccactccggg agttaacctt gtggctctct
     3061 tcggagcgga acacggagtc aggggagcac agcacgggcg catcttcact gacggcgaaa
     3121 aggatcctgt taccgggatt cccgtctata gcctttatgg cgaatcatgg gcgccaaaac
     3181 gtgagtgtct cgacagtata gatgtgatgc tttttgacat ccagggggtg ggatcggcat
     3241 ggtacaccta caagttttca atgtctcatg ccatggaggc atgtgcaaag gcgggcgtcc
     3301 cctttatcgt ccttgacagg cctaatcctc tcggtggccg tattgttgag ggcccgatgc
     3361 acgatacaat aagcatctac cgccatcgcc ttccgctgag gcatgggatg acttacggcg
     3421 agctggcgaa gatgtggaat gagactgaag gctacggagc tgacctcact gtcatcagga
     3481 tgaagggatg gaacaggagt atgatgtgga gcgaaacggg acttcagtgg gtgatgccgt
     3541 cacccaatat ggataactgg gagacagcag tggtataccc gggccagtgc ctcttcgagc
     3601 gaaccaatat gtctgaaggc aggggcatga ccaagccttt tctggtaaca ggtgccccat
     3661 gggtcaatgc cgaacaggct gctgcagacc tgaacgcgag aggaatagcg ggggcatact
     3721 tcaggcctct ctatttcatt cccaggtcat caggtcctgt cattacccgc accagtaagc
     3781 catggaacga gatgtgcggg ggtgttgaga taatactcac tgaccctgct gcttaccgtt
     3841 cggtggaggc ttcactgcat ataattgatg cctacaggaa aaccagcccc gattcgctgg
     3901 tatggaatcc gccaacactg ataaggcggt tgaatgagcc cggggtcacc gttgaggagg
     3961 tggtgaaagc ctgccaggat gatataaggg agttcatgga gacaagacag aaatacctgc
     4021 tctacagatg acgaagaacc tgtttgttat agccctggca acgatgttgc tcattggatg
     4081 cggcactacc gggaggacag ggaagagtgg ggttgaggac ccggcggcca cagctgagaa
     4141 tgcagttgca attgctgcca ggcccacagc tgagaatacg gctgaaattg cagccagggc
     4201 tgcggaaacg cttgcggcaa taccatgccg ctgcgacagc atcggaaaca acccgcctat
     4261 ggttaagttt ctcaggcttc cggagccact gacgcaccag cctcagttcg acatcctgtg
     4321 caagagtgaa taccctgcca ccgtaagtat aaacggtcag gtcgtgaaac agtacaaaac
     4381 aggaatattc tttaagactg ttacattcga tgaaggagtg aacaggatta cggcagaggc
     4441 ggtctggccc gatggccgga aagctgtatg cgagcatgta gtcatctatg agaagagaga
     4501 cctgagccgg cagccattcc cgctctggat cgagaagagg tcattcgagc cgtctgctga
     4561 tatggaagtg cttcccggcg aggtggtgaa ggtgagcttt cgcgcctcga aggggcagga
     4621 ggcatggctg gatatcaaac cgggaaggaa gagaattaaa tgcctgcgtg aggattataa
     4681 tgactacagc ctttaccgtg cagaggtgcc ggtgagggac ttcgccgcag gcgtgccgca
     4741 tacctttgct gtcaggctgg tgcctgcagc cggaggagcc ccggagggcg tcttcaggcc
     4801 tgatttatca aggagggtga cagtccggaa ccccgaggat tttccgcttg tccgggtgaa
     4861 aaatgattat tcgaggcttg tttataacct tggggccccg agactgggcg gaccgataag
     4921 gtcagagctg ggacccgggg tgattctcaa gaccagcggc aagattggca gcaattacag
     4981 gatcaggctc agtaataccg aaacaggatt catcagcgag gatgatgtag agattcaggg
     5041 tggcagcaaa atgcagcctt tgtacaatgt caccagcatg tcatgcggac catcggcccg
     5101 gggtgatgtt gtgaccatac cttatctcgt gccggtgcct tatgaggtcc gtcccgatcc
     5161 tgatagtaaa cgtcttgttg ttacgctttt cggggtggag tcggctgcca cctgggtcac
     5221 ccaccgcacc ggatgccgtg tggttgacaa actcacatgg gagcagagca ccccggaaac
     5281 cttccgggtt tacgtgaacc tgaagagcga aaagatctgg ggttatgaca taagacctgc
     5341 tggcagaagc ctgatcctga ccatcaggta tgccccggta tttgatccgg caggggagaa
     5401 gccgctggcc ggactgaaga ttgccatcga ggccggacac ggcggatcaa actcaggcgc
     5461 aatcggattg tcaggtctcg ttgagaagga gattaacctt gacctgtcat tcaggctcgg
     5521 agagctttgc gactccatgg gcgccgaggt gctgcaggtc agggagagtg atgtggacat
     5581 gaccctgccg gataagcgcg acaaggccat aaaggcaggt gcagatatgc tcatcagtat
     5641 tcatgccaac gcgggaggga gcggttatct ctcagtggca ggcaccagca cctactggca
     5701 taatccgttc tgggcaccgc tggcaaagac catctatgac aggatgctgg agacaggact
     5761 ggcggagttt ggcgtggtgg gatcattcaa ctataccgtc acccgtacat cacagatgcc
     5821 tgcagtactg gttgagcagg ccttcatgag tcatgctgga gacgaagaga aacttgccga
     5881 cccggagttc cgccagctga tggcggtca
//
LOCUS       DAOPBK010000155         5791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_12027_length_5791_cov_6.935844, whole genome shotgun sequence.
ACCESSION   DAOPBK010000155 DAOPBK010000000
VERSION     DAOPBK010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12027_length_5791_cov_6.935844"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PLA17_08905"
     CDS             complement(<1..1145)
                     /locus_tag="PLA17_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPK85574.1"
                     /translation="MTVSRRKKKRFFSARARRKDSGIRRFTRNILLVANLLLVILLVL
                     AYLSIYINPATIAFPALFGLAYPYIAAMNVIMVIVWILFRKWYALLSAIALAAGFGYI
                     HNFIRFTNHGKEEHHDLKLLSYNVRLFNNYESDEKNTQSRVLQLLRTENPGIICLQEY
                     YVRGDPAVGERKLKEGVGGNRYTHFKLIKSGTASRYGIATISRYPIIYRGDVIHPGSS
                     SLTIFSDIVVDTDTFRVYNNHLQSFRLRRMERNLLSELAGEERGRSMDNISDIYKSLM
                     QGFASRALQVDRVRRHMELSPYPVIVAGDFNDTPVSYTYRVMRRGLNDAFVEAGYGAG
                     FTYRGKYPPNRIDYIIYGEGIECTDFDIVKVRYSDHYPVIAYFRRADR"
     gene            complement(1084..1998)
                     /locus_tag="PLA17_08910"
     CDS             complement(1084..1998)
                     /locus_tag="PLA17_08910"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPK85575.1"
                     /translation="MSIWNEIKSSFRRGTTLTRLMYINGAIFLFIKIIEVIGVITSSP
                     DLATAVISNLAVPASTSVLVFKPWTPVTYMFTHQGFIHLLFNLLWLYWFGKIFLAYLD
                     QRKLVSLYIMGGLAGALLYIIVFNLFPAFASAVNISIALGASASVMALVIATAVYLPD
                     LELHLLFIGRVKLKYLALITFLITSVFDFSVNTGGKIAHIGGAVMGFAYGQGLRNGRD
                     FGAWLNRILDFFVNLFRPGKGLRVKYKRAGASFNTKKTKTDYDYNSTKAERQKEIDHI
                     LDKISKGGYDSLTKEEKTILFSESQKKG"
     gene            complement(1998..2720)
                     /locus_tag="PLA17_08915"
     CDS             complement(1998..2720)
                     /locus_tag="PLA17_08915"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPK85576.1"
                     /translation="MNRILSGTPPVVRKLIIINVVMLLLNELLRLTAGINLNGMLGLY
                     FPKSEFFRSWQIVTHMFMHGGFIHLFFNMFALWMFGRILEQVWGPKRFLLYYLVTGLG
                     AAFFFELVQWIQYNKVMAILSPEQIQYIYDVGGYAVSQGNSHMGRLNSILNVPVVGAS
                     GAVFGVLLAFGMLFPNTQLMFIFPPIPVKAKYVVIGYAAIELYLAITSPGSGIAHTAH
                     LGGMIFGYILIRYWRKTTKTLY"
     gene            complement(2773..4617)
                     /gene="mutL"
                     /locus_tag="PLA17_08920"
     CDS             complement(2773..4617)
                     /gene="mutL"
                     /locus_tag="PLA17_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPK85577.1"
                     /translation="MSDIIKLLPDSVANQIAAGEVIQRPASVVKELTENAVDAGATEI
                     SVVIRDAGRTLVQVIDNGCGMSETDARLAFERHATSKISSADDLYIITTKGFRGEALA
                     SIVAVSMTELRTRREEEEAGTLIEINNSRVTRQEPCSCAAGSVFSVRNLFYNVPARRK
                     FLKSDTTEFRHIITEFQRVALAHPEIRFNLTHNDQEVYRLPAGNHRQRIAGLFGKHYN
                     QNLVSVETDTSIVTVKGYAGKPENARRSAGEQFFFVNNRFIRHPYLHRAVLEAYHGLL
                     PPETLPAYFIYLTADPATIDVNIHPTKTEVKFEDERSVWQIMLASVREALGRFNVVPS
                     LDFGPESAVEIPVIIGNTAPPPVPQIEFEHTFNPFEGTEYQRPETKWQWDAERNPPRD
                     WESLFTITTRSGDKDESGSGDRISGGQSADAGEVTGQRRFFQVKNRYIMCHVISGIMM
                     IDQKRAHERVLYEKYLASLDDGPKPAQISLFPVEVELNPADMVIIEEIAGQLRMLGFE
                     ITTGREGIVTITGHPSASRNASPLAMLETIIAEYKHSMEDPAIGERERIALSMARASA
                     IQYGTPLTDTEMEELFDMLFACSLPNYSPSGKIVMNIITLKELDRRFS"
     gene            complement(4627..4995)
                     /locus_tag="PLA17_08925"
     CDS             complement(4627..4995)
                     /locus_tag="PLA17_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85578.1"
                     /translation="MAIPSFFKQNKPRGFNYKPRYYDPVKDELEQRRRAYEEERRKSG
                     EEPENIESSSTETTEDGQPAFAKAAEKRAQQPYRSRIMRGDMRKHFNRRHERVQKHSN
                     IRLIVILIILAFVVYLYLRF"
     gene            5116..5661
                     /locus_tag="PLA17_08930"
     CDS             5116..5661
                     /locus_tag="PLA17_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPK85579.1"
                     /translation="MKILVTHAGTSGQAAGMAERVARVLTGLGADVTVRPMSGVATVK
                     GFSAVVAGSPEHGGCWVPEAIEFVRRNRSVLSRKPFAIFTVVSSQDMDKGAESRMVVM
                     QYTAHVRSMVHPMSEGFFSGEAEERESPHPGERSRFKLRLLLGLLREGESRSHSEVET
                     WSVRLHEQLVRLISRKSGNRQ"
BASE COUNT         1301 a         1417 c         1590 g         1483 t
ORIGIN      
        1 cggtcagcgc gcctgaaata ggcaatgacc gggtagtgat ctgaatacct gaccttgaca
       61 atgtcaaaat cagtacactc aattccttcg ccgtagatga tatagtctat ccggttgggc
      121 gggtacttgc cgcgataggt aaacccggcc ccgtaacccg cctcaacaaa tgcatcgttc
      181 aatccgcggc gcataacgcg gtatgtatat gaaaccggcg tgtcattgaa atcgcctgca
      241 acaatcaccg ggtagggcga caactccatg tgtcttctta cacggtcaac ctgcagggca
      301 cgactggcaa atccctgcat caggctcttg taaatatctg agatgttatc catggagcgc
      361 cctctctcct caccggcaag ctcgctgagc agattccttt ccatgcgccg caggcgaaac
      421 gactgcaggt ggttgttgta aaccctgaaa gtatcagtgt caacaacgat atcgctgaag
      481 atggtcagtg atgatgaccc cggatgaatg acatcgcccc tgtaaatgat cggatagcgt
      541 gatatggtag caataccgta ccggctggcc gttccgctct ttatcagttt gaagtgggtg
      601 tatctgtttc ctcccacgcc ctccttaagt tttctctcac caactgccgg gtcacccctg
      661 acataatact cctgcaggca gataatcccc ggattctccg tcctcagaag ctgaagcacc
      721 ctgctttggg tgttcttctc gtctgactca taattattga acagacgtac gttgtaactc
      781 agcaacttaa ggtcatgatg ctcctccttc ccatggttgg tgaacctgat gaagttgtgg
      841 atatacccga atcctgccgc aagtgcaatt gcagacagca gcgcatacca tttcctgaag
      901 aggatccaga cgatgaccat gataacgttc atggcagcga tataggggta tgcaagcccg
      961 aaaagggcag ggaatgctat cgttgcagga ttgatataga ttgaaaggta tgccagcacc
     1021 aggaggataa ccagtaaaag gttggccacc agcagtatat ttcgcgtgaa gcgcctgata
     1081 ccgctatcct ttcttctggc tctcgctgaa aagaatcgtt ttttcttcct tcgtgagact
     1141 gtcataacct cccttagaaa ttttgtcgag tatatgatct atctccttct gcctttcggc
     1201 ttttgtggag ttgtaatcgt aatcggtttt cgtctttttt gtattgaagg atgctccggc
     1261 cctcttgtat ttcaccctga gccctttccc cggtctgaac agattaacaa agaagtcaag
     1321 aatcctgttt aaccatgctc caaaatccct gccatttctc aaaccctgcc cgtatgcaaa
     1381 tcccatcacc gcaccgccga tgtgggcgat cttgccgccg gtattaaccg aaaaatcaaa
     1441 tactgaggtg ataaggaagg ttatcagcgc caggtattta agcttcaccc ttccgataaa
     1501 caggaggtgt agctcgaggt cgggaaggta aacagccgtg gctattacca gcgccattac
     1561 cgaggctgag gcacccagcg cgattgagat atttaccgct gacgcgaagg ccgggaagag
     1621 attgaagaca atgatgtaaa gaagcgcccc ggcgaggcct cccatgatat aaagtgagac
     1681 cagcttccgc tgatcgaggt atgccaggaa aattttaccg aaccaataga gccacagaag
     1741 gttaaagagc aggtgaatga atccctggtg ggtgaacata taggtgaccg gggtccatgg
     1801 cttgaagacc aggacggagg tggatgccgg gactgccagg ttcgatatta ctgccgttgc
     1861 caggtcggga ctggaggtaa tgacccctat tacttcaatg atcttgataa acagaaagat
     1921 ggcgccgtta atatacatca gccgcgtcag ggtggtgcca cgtctgaatg agctctttat
     1981 ttcgttccag atgctcatca gtaaagggtt ttagttgtct tgcgccagta ccttatgagg
     2041 atgtagccga agatcattcc tccgaggtgg gcggtatggg ctattccgct tccgggactg
     2101 gtgatggcaa ggtaaagctc aatggcagca tacccgatga caacgtattt tgccttaacc
     2161 ggaattggcg gaaatatgaa catcagctga gtgttgggga atagcatccc gaaggccagc
     2221 agcacaccaa agacggcgcc tgatgctccc acaacgggta cgttgagaat cgagttaagc
     2281 cttcccatat ggctgttgcc ctggctgacc gcataaccgc caacgtcata tatgtattga
     2341 atctgctcag gagagagtat tgccatcacc ttgttgtact ggatccattg cacaagctca
     2401 aagaagaagg ctgcaccgag gcctgtcacc aggtaataga gcaggaaccg cttcggtccc
     2461 cacacctgct cgagtatcct cccgaacatc catagtgcga acatattgaa aaacagatga
     2521 ataaatccgc cgtgcataaa catgtgtgtt actatctgcc atgagcggaa gaactccgat
     2581 ttagggaagt aaagccccag catgccgttc aggttgatgc ctgcggtcag cctgagcagt
     2641 tcattaagca ggagcataac cacattgatg atgatgagtt ttctcaccac cgggggagtt
     2701 cctgaaagta ttctgttcat cttgttgttt tcacatattt cagtccgcaa atgtaggcat
     2761 tctgcccgaa tatcagctga atctccggtc aagctccttg agggtgatga tgttcatcac
     2821 tattttgcct gagggagagt agtttggcaa agagcaggca aagagcatgt caaagagttc
     2881 ctccatctct gtgtctgtca gcggggtgcc gtactggata gctgaagccc tggccatgga
     2941 gagggcaatc cgttcgcgtt cacctatggc cgggtcttcc attgagtgct tgtattcagc
     3001 gatgattgtt tcgagcatcg ctaaggggct tgcattgcgg cttgcagagg gatggccggt
     3061 gatagtcaca atgccttccc tgccggttgt tatctcaaat ccgagcatcc tgagctggcc
     3121 ggcgatctct tcaatgataa ccatgtctgc cgggttgagc tccacttcca ccgggaaaag
     3181 cgatatctgt gcaggtttcg ggccgtcgtc gagcgaggca aggtacttct cgtagagcac
     3241 tctctcatgc gctctcttct ggtcaatcat cataatgcct gagatcacat ggcacattat
     3301 gtagcggttc ttcacctgga aaaaacggcg ctggcctgtc acctctccgg catctgctga
     3361 ttgcccgcct gatattctgt ctccgctccc gctctcatcc ttgtctccgc tgcgggtggt
     3421 aatggtgaaa agcgactccc agtcgcgggg aggattgcgt tcggcatccc attgccattt
     3481 cgtttcaggt ctctggtatt cggttccctc gaagggattg aaggtgtgtt caaactctat
     3541 ctgcggcacc ggtggaggag cggtgttgcc aatgataacg ggaatttcca cagcgctctc
     3601 aggtccgaag tcaagcgacg gaacaacgtt gaaccggccc agcgcctcac ggacggatgc
     3661 cagcatgatc tgccagacag acctctcatc ctcgaacttg acctcggttt tggtggggtg
     3721 aatattgaca tctattgttg ccgggtcggc ggtcaggtag atgaagtatg ccggcagtgt
     3781 ctccggagga agaaggccat ggtaagcctc aagcacggcg cggtggaggt atggatgcct
     3841 tatgaacctg ttgttcacaa agaagaactg ttccccggcg gagcggcggg cattttcagg
     3901 cttccccgcg taacctttta cagtcactat ggaggtgtcg gtctccacgc ttaccaggtt
     3961 ctggttgtag tgtttaccga acagtcctgc tatacgctga cggtgatttc ccgccggcag
     4021 gcggtaaacc tcctggtcat tgtgggtaag gttgaaccgt atctcagggt gggccagcgc
     4081 cacacgctgg aactcggtga tgatatgcct gaactctgtt gtgtctgact tcaggaattt
     4141 gcggcgggcg gggacattgt aaaataggtt cctgacgctg aatactgatc ccgcggcgca
     4201 tgagcatggc tcctgtctgg taactctcga gttgtttatc tcaatgagtg ttcctgcctc
     4261 ttcttcttcg cgccttgtgc gcaactcggt cattgagact gctacaattg aggcgagtgc
     4321 ctcgccccgg aatcctttgg tagttatgat atagaggtca tcagctgatg agatctttga
     4381 ggtagcgtgt ctttcgaatg cgaggcgggc atcggtctcg ctcatgccgc agccgttgtc
     4441 aatcacctgt accagggtgc gtcccgcatc gcggataacg acgcttatct ccgtggctcc
     4501 tgcgtcaacg gcgttctcag tcagttcctt gaccaccgag gccgggcgct ggataacttc
     4561 gccggctgct atctggttgg cgacggagtc gggcaggagc tttatgatgt cactcatgcg
     4621 gtgcgattaa aaacggagat aaagatagac tacaaaagcc agaataatca gtatcacaat
     4681 cagcctgatg ttggaatgtt tctgcactct ctcatgtctg cggttgaagt gttttctcat
     4741 gtcaccgcgc attatcctgg agcggtatgg ttgttgggct ctcttctccg cagctttagc
     4801 gaaggcgggc tgtccgtcct ccgtagtttc agtggaggag gactcaatat tttctggttc
     4861 ttccccgctt tttctcctct cttcctcata agccctgcgt ctctgttcca gttcatcctt
     4921 aaccggatca tagtaccgtg gcttgtagtt gaagccgcgg ggtttgttct gtttgaagaa
     4981 actcgggata gccattgtgc tctttttgca aaaatagcaa gaaaacggtt aacggggaat
     5041 ttcaggccgc tttcccggag ttaaaggtag ttcacttttt attacttttg ggaatctaaa
     5101 ccttgttcgg gagcgatgaa gattcttgta acccatgcgg ggacatcagg gcaggcggcc
     5161 ggtatggctg agcgtgtggc acgggtattg accggtctgg gagctgatgt aactgtcagg
     5221 cccatgagcg gtgtggcaac ggtaaagggt ttttcagctg ttgttgcagg gagcccggag
     5281 catggtggat gctgggttcc ggaggccatt gagtttgtca ggcgaaaccg gtcggttttg
     5341 agcaggaagc cgtttgccat attcactgtc gtttcgtcgc aggatatgga taaaggtgct
     5401 gagtcgagga tggtggtcat gcagtacacg gctcatgtga gaagtatggt tcacccgatg
     5461 agtgaaggtt tttttagtgg tgaagctgaa gagcgggagt caccgcatcc tggtgagcgg
     5521 tcgaggttca agttgcggct tttgctcggg ttacttcgcg agggtgagtc acggagtcat
     5581 tcggaggttg agacctggtc agtccggttg catgaacagc tggtcaggtt aatctccagg
     5641 aaatcaggca acaggcagta ggcgggagtg ggcaggcagt tctcagcgca cggcagacaa
     5701 cagatggcgg gcggttggct gcaggtggtt agcgtttggc tggagtaagg tctgcaggtt
     5761 tcggttggca tttggcagcg cgcggcaggg g
//
LOCUS       DAOPBK010000156         5615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_12436_length_5615_cov_3.635791, whole genome shotgun sequence.
ACCESSION   DAOPBK010000156 DAOPBK010000000
VERSION     DAOPBK010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12436_length_5615_cov_3.635791"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PLA17_08935"
     CDS             complement(<1..876)
                     /locus_tag="PLA17_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85580.1"
                     /translation="MMIRRTILSAVMIMIAALVTGQKTDMSLFDGIKPRNIGPGGMSG
                     RVTAFAVDPRNENVFYVGTASGGLWKTLNAGTTFTPIFDNEAVASIGALAVDPQRPDI
                     VWAGTGEGNPRNSVTGGYGIYKSLDGGRTWKCVGLELTRYIHRIIVDPVNPDVIYAGA
                     IGNPWAPHTERGFYRSTDGGGTWNRLLFTNETSGVAYMVMDPSNPGKIFVAMWDHLRW
                     PWFFRSSSAGSGLWLTRNGGNTFERVSGGLPEETGRIGLAIARSNPDYVYAYVESKPS
                     AIYRSTDGGVNWEKRG"
     gene            complement(955..1656)
                     /locus_tag="PLA17_08940"
     CDS             complement(955..1656)
                     /locus_tag="PLA17_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPK85581.1"
                     /translation="MSGTLYMIPVSLGEADPNLSIPPGVRDITLSIRLFAVEEVRTAR
                     RWLRSLDRTFPIDSTLFFPIGKHSDAAGLEDFFVRVSGGSDAGVMSEAGMPGLADPGN
                     IIASEAHRRGIRVVPLTGPSSVMLALVASGLNGQSFSFHGYLPVDPQGRRKAIRELER
                     LSAGGSTRIFMETPFRNGKMIKDILAVCQPSTRLCIAADISLPTEFIRTMSVAEWRSQ
                     VPELEKRPAIFLIQA"
     assembly_gap    1662..1761
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2225..2992
                     /locus_tag="PLA17_08945"
     CDS             2225..2992
                     /locus_tag="PLA17_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK85582.1"
                     /translation="MKITFLGTGTSQGVPVIACECGVCTSPDPKDKRLRASLLLETAL
                     NTFVIDAGPDFRQQMLNARVKKLDAILLTHEHKDHIAGMDDLRAFNHKSKAAIDIYAE
                     ERVQAAVRKEYAYVFSENHYPGVPKMELHNIGNYNLTIKGDTFTPLRIFHYRLPILGF
                     RIGNMAYITDANYIPEETKEQLTGVKYLVINALRKEKHVSHFSLGEALDLVREVSPRK
                     AYITHIGHQMGRHAEVNRELPVNVTLAWDGLEVNCED"
     gene            complement(3124..3333)
                     /locus_tag="PLA17_08950"
     CDS             complement(3124..3333)
                     /locus_tag="PLA17_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85583.1"
                     /translation="MKKVNSKAITLIILILSAILLMPGCATSKKNSYKAARKGSYIDT
                     TQLGRNKYFFSKKYQKKLYRYKKKK"
     gene            3587..>5615
                     /locus_tag="PLA17_08955"
     CDS             3587..>5615
                     /locus_tag="PLA17_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85584.1"
                     /translation="MGIIHDNGASAVSLMLDDSSVVKLAVPRPLFSFRLDDAYFESDD
                     VPAGLVANRFLMVYPGGAEAGYGPRGGSHPGFMAELVLRNIWNDTITVTNVVPFGEKE
                     NSAFITGAGPRDLARANLFLPGKSPVRVILPDNAWEMGFSTFDAAPGLSVASIARRIS
                     SDRGITRRYETLLPPGASVTYHIHADLYRGEWQEGLRLMFAGRWLHDLETFDEVMYNR
                     EDLKWVRTSYLMILQMAWDRELRDRFTGKEAFEGYLAEYNRLFGHLDVYGIWPTWPRL
                     GLDDRNQWDLYRDLPGGTAALRRLSREARSQGCRFFIAYNPWDKSTRGEDHLSNMAQI
                     IAETEADGVVLDTEGSSSPELQQAADTVRSGVVMYSEGMPVVRDMPGIVAARVHNALW
                     LSPELNLNKVIRPDFAIFRVADVGEDVLHREISVAFFNGYGTELNMFRPGGRGDEFRK
                     DLNYLAQTTYMLRRNSDAFAGGNWTPLIGSGADRVYVNSWNAGEKQVFTILNMDHRGY
                     KGSLFSPGDTTGKHLVSLFRHEELTPAVINNTLTAPVDLPGWSQAFNGTRRESSIDCV
                     ALLPKLIIAEFRGSKIRIASDVSGMLLVSRGDPGYTNKPMRITAPADTMIYADALLGI
                     AGEKIVIELTDSDGILLDERVIRPEQGRPWLVSATERTLPASEIPEGMVLVP"
BASE COUNT         1323 a         1455 c         1434 g         1303 t
ORIGIN      
        1 accgcgcttt tcccagttca ccccgccgtc ggtggaacgg tatatggctg acggctttga
       61 ctcaacatag gcataaacat aatcaggatt tgatcttgct atggccagcc cgatgcgccc
      121 ggtctcctcc ggaagtccgc cactgaccct ctcgaaggta ttacctccgt tgcgggtaag
      181 ccacaggcct gatcccgctg acgacgaacg gaagaaccat ggccagcgca ggtgatccca
      241 catggccaca aagatctttc cggggttgga aggatccatg accatgtaag ccactccgga
      301 ggtctcattg gtgaagagca gcctgttcca ggtgccccct ccgtcagtgg aacggtaaaa
      361 gcctctctcg gtgtgtggcg cccagggatt gccgatggca ccggcataga ttacatcagg
      421 attgacaggg tcaacgatga tacggtgaat gtaccgggtc agttcaaggc ccacgcactt
      481 ccaggtacgg ccgccgtcaa ggcttttgta tatgccgtag cctccggtga cactgttgcg
      541 cggattgccc tcgccggtgc ctgcccagac aatgtcaggg cgttgaggat caacagccaa
      601 cgcacctata gaggcaacag cttcattatc aaatataggt gtgaaggtgg tacccgcgtt
      661 taatgttttc cagagccctc ctgaggcagt gcccacgtaa aacacatttt cgttccgcgg
      721 gtcaacagca aaggctgtaa ccctgccgct catgccaccc ggaccgatat tcctgggttt
      781 gatgccgtcg aaaagtgaca tgtcagtttt ctgccctgtc acaagcgctg caatcattat
      841 cattacagct gataagatgg ttcttctgat catcattgtc tctgttttac ggacacaatt
      901 tagataaata atcgaattgg ttcagtatgg ctgtaggtgt tgtgcaatac cctttcaggc
      961 ctgaatcagg aagattgccg gcctcttttc cagctccggt acctggctgc gccactctgc
     1021 cacgctcatg gtacgtatga actctgtcgg gagggagatg tcggctgcaa tgcacaacct
     1081 ggttgagggc tgacagactg caaggatatc ctttatcatc tttccgttcc tgaatggtgt
     1141 ctccatgaag atccgggtac tgccgccggc ggagagcctc tccagctctc tgatagcctt
     1201 gcggcggccc tgcggatcaa caggcaggta accatggaac gagaacgact ggccattcaa
     1261 tcctgatgca accagcgcga gcatgaccga tgagggaccg gttagcggca caaccctgat
     1321 gccacgccgg tgggcttcag aagcaattat gttaccaggg tcagccaggc ccggcatacc
     1381 cgcctcactc atcacccctg catcagaacc gccggacacc ctgacgaaaa aatcttccag
     1441 gcctgccgcg tcagagtgct tgccgatagg gaagaagagg gtactgtcaa tggggaaagt
     1501 cctgtcaagg ctcctcagcc atctcctcgc cgtccgtact tcctctactg caaatagcct
     1561 gatagacaac gtgatgtcac gtactcccgg gggaatggag agattcggat cggcctcacc
     1621 cagtgaaacc ggaatcatat agagtgttcc gctcatgtca annnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nggatcggcc tcacccagtg aaaccggaat catatagagt
     1801 gttccgctca tgtcaatgac tgattgtacc gtaaacttac ataaaatcaa ccttttttca
     1861 caaatccctg tattcatgac tgaatcaatc cgccaaaaaa gagcatgacc ggaggctctc
     1921 gttaccgtga tctggagact cctgtactcc ggacctgtct ggtcacctgg tttccggact
     1981 tattctgctc ctgagcccct ggtccccgga cttaaagact cctgtactcc ggacctgtct
     2041 ggtcactggt cacctggtcc acagacccgg gggctccgat tcccctgacc cggatctgag
     2101 ggctcctggt ccccgaatct gcagattcct gctcccctga cctggaagct cctgctcaag
     2161 ggtgatacct gatattgtac ttttctcaat ttcaggttat gtttgcggtg acaaaaatga
     2221 gacagtgaaa atcacctttc ttggcaccgg cacttcccag ggtgttccgg taatagcttg
     2281 tgaatgtggt gtctgtactt cgccagaccc gaaggacaaa agactgagag cttccctgct
     2341 cctggagact gccctgaata ctttcgtcat tgacgccggt cctgacttca ggcagcagat
     2401 gcttaacgcc cgggtgaaaa agcttgacgc catccttctt acacatgaac acaaggatca
     2461 tatcgccggg atggatgacc tccgggcttt caaccataaa agcaaggcag ccattgatat
     2521 ctatgctgag gagagagtac aggcagcagt cagaaaagaa tacgcctatg tcttttctga
     2581 aaaccattac cctggcgttc ctaaaatgga actccacaat atcggtaact acaatctgac
     2641 aataaaggga gataccttta ctccgctccg gatatttcac taccgcctgc caatacttgg
     2701 tttcaggatc ggtaacatgg catacattac tgacgccaac tatatccccg aagagacaaa
     2761 ggaacagctg acaggtgtca aatatctggt tatcaatgct ctcagaaaag agaagcatgt
     2821 ctctcacttc agccttggcg aagccctcga cctggtgcgg gaagtatcac cccggaaagc
     2881 atatattacg catataggac accagatggg caggcatgcc gaggtgaaca gggaacttcc
     2941 ggtcaacgtg acactggcct gggacggcct tgaggtgaat tgtgaagatt gaaattcctg
     3001 aatttacaca gcttatacct catccttgag caacttgagg taaaatgtga agattgaaac
     3061 tcatcctgct gccacgggca tcccggcctg ggcagacagg aggcagcatt cctgaccctg
     3121 tgcctatttt ttctttttgt atctgtaaag ctttttctgg tacttcttcg agaagaaata
     3181 tttgtttcgc ccaagttgtg tggtgtcaat gtatgagccc ttgcgtgcag ccttgtatga
     3241 gttcttcttt gaggttgcac agccaggcat cagcaagatt gctgacagta tcagaataat
     3301 gagagtgatg gctttgctgt taaccttttt catcagattg cgctgaacta attcctgaaa
     3361 gcaggtataa acgtgtaacg tgcttttatg ggtaaatatt atctctaaaa taattcactt
     3421 actgtatctt cgcttttatt ttcagataca aactcataaa ttgcattccc atttcatcag
     3481 gaagcaaatt atatcagttt aaactcctat gtgcagcttt aggtttcggt tttccctctt
     3541 ttttttaatt gcctgccttt cgcttaacgg gcagtcgctt attaatatgg gaattataca
     3601 tgataatgga gccagtgccg tctcgctcat gcttgacgac agctccgtgg taaagcttgc
     3661 cgttccccgt ccgctcttca gcttcaggct tgacgatgca tattttgaat cagacgatgt
     3721 acctgccggc ctcgtcgcaa accgtttcct gatggtttat ccgggaggtg cagaagccgg
     3781 ctatggtccg cggggcggat cgcatccggg attcatggcc gagctggtgc tgcgcaatat
     3841 ctggaatgat accattaccg taaccaacgt ggtgccattc ggcgaaaagg aaaacagtgc
     3901 atttatcacg ggagccggcc ctcgtgacct ggcaagggca aacctcttcc tgcccggcaa
     3961 gagccccgtc agggtaatac tccctgacaa tgcatgggaa atgggcttca gcaccttcga
     4021 tgcagcaccg ggactctcgg tggcttccat tgcaaggcgg atcagttccg acagaggaat
     4081 caccaggcga tatgagacct tacttcctcc gggagcatca gtgacctacc acatccatgc
     4141 agatctgtac aggggtgaat ggcaggaggg actcagactc atgttcgccg gacggtggct
     4201 gcatgatctg gagacttttg atgaagtcat gtacaacaga gaggacctga aatgggtacg
     4261 caccagctac ctgatgattc tccagatggc atgggacagg gagctgcgtg accgcttcac
     4321 cggcaaagaa gcgtttgaag gctacctggc tgaatacaac aggctgttcg gccacctcga
     4381 tgtttacggt atatggccta catggccgcg cctggggctt gacgacagga accagtggga
     4441 cctctaccgt gacctgccgg gaggaacagc cgctctcagg cgcctcagca gggaggcaag
     4501 gtcacaggga tgccgcttct tcatcgcata caatccgtgg gataaaagca cccgcgggga
     4561 ggatcacctc agcaatatgg cacagataat cgcagaaaca gaggcagatg gcgtggtgct
     4621 tgataccgaa ggcagctctt cgcctgaact gcagcaggct gccgataccg ttcgcagtgg
     4681 cgtggtgatg tactccgagg gaatgccggt ggtcagggac atgccgggca tcgtggctgc
     4741 aagagtgcat aatgccctct ggctcagtcc tgaactgaac cttaacaagg tgatcagacc
     4801 cgactttgcc atcttccgtg tggcagacgt tggtgaggat gtgcttcacc gcgagatttc
     4861 ggttgcattc ttcaacggat atggtactga actgaacatg ttcagaccag gcggcagggg
     4921 tgatgagttt agaaaagacc tgaattacct ggctcagaca acatatatgc tcaggagaaa
     4981 cagcgacgcc tttgccggcg gtaactggac accattgata ggcagcggtg ccgacagggt
     5041 ttatgtcaac agctggaatg ccggtgaaaa gcaggtgttt actatcctga acatggatca
     5101 tcgcggatac aagggctccc tgttcagccc cggcgatacc accggcaaac acctggtatc
     5161 actcttccgg catgaggagc tcacccccgc ggtaatcaac aacactctta ctgccccggt
     5221 tgaccttcca gggtggagcc aggcattcaa cggaacgagg cgcgagagct ccattgactg
     5281 tgtggcgtta ctgccaaaac tgatcattgc cgagtttcgc ggcagcaaaa tacggattgc
     5341 ctctgatgtc agcggcatgc tgctggtaag caggggagat ccgggttata caaacaagcc
     5401 catgcggata accgctccgg ctgataccat gatctatgcc gatgccctgc tgggaatagc
     5461 gggcgaaaag atagtgatag aactgactga cagtgatggt atactgcttg atgagagggt
     5521 gataaggcct gagcagggca gaccatggct tgtctcggca actgaacgca ccctgcccgc
     5581 atctgagata ccggaaggga tggtgctcgt ccccg
//
LOCUS       DAOPBK010000157         5499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_12706_length_5499_cov_5.119949, whole genome shotgun sequence.
ACCESSION   DAOPBK010000157 DAOPBK010000000
VERSION     DAOPBK010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12706_length_5499_cov_5.119949"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLA17_08960"
     CDS             <1..304
                     /locus_tag="PLA17_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPK85585.1"
                     /translation="VPLVAGAMGMYEVVTPDMLRIAADPAYAAFFVQDGLFWEFLAYC
                     AGTGGSMLIIGSAAGVAAMGLERIDFIWYLKKISWLALAGYLAGAGVYWLQAQIMV"
     gene            complement(488..1900)
                     /locus_tag="PLA17_08965"
     CDS             complement(488..1900)
                     /locus_tag="PLA17_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPK85586.1"
                     /translation="MKNLILAMAAGNRKRVLLFLLLLLLPVTIFASDGSVPKIGPIRV
                     EFIIFGLILVGVALFHRHTFWVAVTGLTVLLIFKFAFDPAFRLGEHMFGTTPFLEQIM
                     DKDLRQGEWGIILNLLGLLLGFAVLSKIFEESRVPDILPRYLPDDWKGPFLLLVFVFI
                     LSSFLDNIAAALIGGTIALVVFKNRVHIGYIVAIVAASNAGGSGSVVGDTTTTMMWID
                     GVSAFNVLHAYVAAVVALLFFAWFASHQQDRHQRIQKDADASVRIDWVKIVCVFLILA
                     GAITSNILYDMPALGVWIAIIIGAFLRPVPWGEIPASLKGTVFLLCLVMCASMMPVEE
                     LPDASWVTTFALGFLSAVFDNIPLTKLCLEQGHYDWGMLAYAVGFGGSMIWFGSSAGV
                     AITNKFQEARNVVLWVRKGWHVIVAYVLGFFALYLLLGWEPADSREHKIINCPVPGCP
                     MAGPGEAAEGTISYLDLINK"
     gene            complement(2255..2560)
                     /locus_tag="PLA17_08970"
     CDS             complement(2255..2560)
                     /locus_tag="PLA17_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPK85587.1"
                     /translation="MNIFVAKLSSVTKAEDLQELFTKFGEVISAKVIMDRETGFSKRY
                     GFVEMADDRAGYEAIEKLNNSELDGSQIIVKKSEPKAEKKPFRPRRDFRDRDHEPKY"
     gene            complement(2746..4041)
                     /locus_tag="PLA17_08975"
     CDS             complement(2746..4041)
                     /locus_tag="PLA17_08975"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HPK85588.1"
                     /translation="MFRLNLFVLMVAAILTTGCSKKIMTLEERLASLPADTLFRIKAD
                     TSFSEAWEIRLRQPVDHRNPDGPQFTQQVFLYYAGADRPVVVETEGYGARGGRNELAS
                     ILNANLIRIEHRYFEESTPDPVDWKYLTTWQAATDHHRIIELFKPVFRGRWATTGISK
                     GGQTVMFHRYYYPDDVDVSVPYVAPLNFGPEDSRMEPFFENVGTAECRDRVFRFQKMA
                     YEKFDTLYPMFLEMADSRGWTFTRVGGPEIAYEMTVLEYEFAFWQWGNVSCDSIPLEG
                     NNEQIFRHMTSVSPPRYFSDQDLEYFEPFFYQAMTEIGYYGYDFDKFGDLIRHAGDGE
                     EPEFQFSAPPGLDYVYDYEFGEKVSEFIKHDAKNFIFIYGENDPWSASAADPGNNKKC
                     LKIVKKDGAHGTRIGTLPDEQKNLVMTKLQEWMKFEVNH"
     gene            complement(4070..>5499)
                     /locus_tag="PLA17_08980"
     CDS             complement(4070..>5499)
                     /locus_tag="PLA17_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HPK85589.1"
                     /translation="KLLISSLKASGATLNLKADLITPLWKDRPDLPGDSAFELDVKYA
                     GLTRHEKLERVREAMKIMKVDYQLLTATDDIMWLLNLRGNDVKYSPLLLSFAVVSLKQ
                     VIFFADEDKIPHEVKASLDSDSVVLLPYETVTSVLSHLEEGSVVLLSPPSTPAALYRA
                     LPRSVDIAEDLSIPTRMKAVKNEVELKNLRKVMVDDGVVLTRFFFWLEKSVGREEITE
                     MSAAAKIDEMRSTQTGCTGPSFSTIAAYNEHAALPHYVPDMKTDVRLKKEGIFLLDSG
                     GQYYGGTTDITRTVALGKPDARMKKEFTLALRGTIDLAMARFPRGTKGYQLDILARKP
                     LWDNYMNYGHGTGHGVGSFLNVHEGPQTIGTSASGDMKTSMEPGMVISDEPAFYRPGH
                     YGFRTENLLVCCTDTESEFGSFLSFETVTLCYIDRELIDISLLDDSELQWLNGYHEKV
                     YRSLHRYLEPELQIWLRAKTMPLSR"
BASE COUNT         1467 a         1389 c         1253 g         1390 t
ORIGIN      
        1 cgttccgctg gtggccggag caatgggaat gtacgaggtg gtcactcccg acatgcttcg
       61 catagctgct gatccggctt atgcagcctt ctttgtgcag gacggactct tctgggaatt
      121 ccttgcttac tgtgccggaa ccggtggcag catgctgatt atcggctccg ccgcaggtgt
      181 tgctgccatg ggccttgaaa gaattgactt tatctggtat ctgaagaaga tttcatggct
      241 tgctcttgca ggatatcttg caggagcagg tgtttactgg cttcaggcac agatcatggt
      301 atgacattgc cgtgcaatag gattttgccc cggcggcaaa cagaatgaca ataaaaaaga
      361 ggctgtctca tattaaaagg ggcagcctct ctttttttga cagttgtcct ggttactcag
      421 gaagcttttg gacaggcacc tctgaaaggt gcccttttaa ttaacggcat caggtaagat
      481 tccttatcta tttgttgatc agatcaaggt agctgattgt tccctccgca gcctcacccg
      541 ggccagccat cgggcacccg ggtacaggac agttgatgat cttatgttcc ctgctgtctg
      601 ccggttccca tccgagaagc agatataggg caaagaatcc cagtacatag gcaacaatca
      661 catgccatcc cttccttacc caaagaacca cattccgtgc ctcctggaac ttgttcgtga
      721 tggctacccc ggcagatgaa ccaaaccata tcatcgatcc tccgaaccct acggcgtatg
      781 ccagcatacc ccagtcatag tgcccctgtt caagacagag ctttgtgagc gggatattat
      841 caaacacggc tgaaaggaac ccaagtgcaa aggtggtcac ccatgaggca tcaggaagct
      901 cttcaaccgg catcatcgat gcgcacatta cgaggcacaa caggaagacg gtacccttaa
      961 gcgaagcagg tatctccccc catggaacag gtctcaggaa ggcgccaata ataattgcta
     1021 tccagacacc cagtgcaggc atgtcataga gaatattgga agttattgca cctgccagaa
     1081 taaggaagac acagactatc ttaacccagt caattctgac actggcatca gcgtcctttt
     1141 gtatcctctg atgcctgtcc tgctgatgag atgcaaacca ggcgaagaac agcagggcaa
     1201 caacggcagc cacgtaggca tgcagaacgt taaaagcaga cacaccgtca atccacatca
     1261 ttgtggtggt tgtatcgcca actacacttc cgctgccgcc tgcattgctt gcagctacaa
     1321 tggcgacaat atacccgatg tgtaccctgt ttttgaatac aaccagcgct attgtccctc
     1381 ctatgagagc agcggcaata ttatcgagga atgatgagag gatgaagaca aagactaaca
     1441 gcaggaaagg acccttccag tcgtcaggaa gatacctggg aagtatgtcg gggaccctgg
     1501 attcttcgaa tatttttgac aggactgcaa agccaaggag caatccgaga agattcaaaa
     1561 ttatccccca ctcgccctgt ctcagatcct tatccatgat ctgctcaagg aagggagtgg
     1621 tgccgaacat atgctcgccc agcctgaagg ccggatcaaa tgcaaacttg aagatcagca
     1681 gaacggtaag gccagtgact gcaacccaga atgtatgcct gtgaaagagc gccactccta
     1741 cgaggatcag gccaaaaatg ataaattcaa cgcgtatcgg ccctatcttg ggcactgaac
     1801 catctgaagc aaaaatggtt accggaagaa ggagaagcag caggaaaagc aacactcttt
     1861 tcctgttacc tgcagccatg gcaagaatta agtttttcat ctttaaagag ttatgcgaat
     1921 ttgttgacgg atctgatcgg ataatatgaa ttagtcgggg cgaaattata gttattttcc
     1981 tttaaaggtg ttcattctcc ctgctttttt taaacctgct gtatgttata aaaagaaagg
     2041 cagagcttct ctgtcttctg caacaaaaaa gaaaggcagc gctcctctgc cttttgcaat
     2101 aaaaaaaaga gagagctctt ctgccgtccg ggagcttcct gagtgatcag gataactgtc
     2161 aaaaaagaga gactgccctt tttaatatga gacagcctct tttacaacaa ataaaaaaag
     2221 ctgccccatc ggacagcttc ttccctttta tttcctagta tttcggctca tggtccctgt
     2281 cgcggaaatc acggcggggt ctgaaaggct ttttttctgc ctttggctca gacttcttca
     2341 cgatgatctg actgccatcg agctctgagt tgttaagctt ttcgatagct tcataaccgg
     2401 cacggtcatc ggccatctca acgaaaccgt aacgctttga aaagccggtt tcacgatcca
     2461 taataacttt ggcggagatt acttccccaa atttggtgaa aagttcctga aggtcttctg
     2521 ctttggttac agaactcaac ttggctacaa aaatgttcat ctgaaaacaa attaaactgt
     2581 taattaagat ttaaattaac tgactgaaaa tgagtgagac actaaaccat ctctacacaa
     2641 aataagacag cttaacacaa aggtatttaa attatcctaa catctccaaa tttattttaa
     2701 aaattgaagt taacaggctg aaagaaaatc tgctaataca atcggtcagt ggtttacctc
     2761 aaacttcatc cattcctgaa gtttagtcat gaccagattc ttctgttcat caggaagggt
     2821 gccgatgcgg gtaccgtgcg ccccatcctt ctttacaatc ttcaggcatt ttttgttatt
     2881 acccggatca gcggcagaag ctgaccaggg atcattctca ccatagatga aaatgaagtt
     2941 tttagcatca tgtttaataa actcgctgac tttttccccg aattcataat catatacata
     3001 gtccagcccc ggaggagcag aaaactggaa ttcaggttct tccccgtcgc cggcatgcct
     3061 gatgagatca ccaaacttgt caaagtcata accataatac cctatctcag tcattgcctg
     3121 ataaaagaag ggttcaaaat actccagatc ctggtcagaa aaataacggg gaggactcac
     3181 cgatgtcata tgtctgaata tctgctcatt attgccttcg agaggtatgg aatcacagga
     3241 gacgtttccc cattgccaga acgcaaactc atattcaagc actgtcatct cataggcaat
     3301 ttcgggccct cctaccctcg tgaatgtcca tccccgactg tcagccattt caaggaacat
     3361 gggataaagg gtatcaaatt tttcataggc cattttttgg aaccggaaga cccggtcacg
     3421 gcattcagct gtgcccacgt tctcaaaaaa cggctccatt ctggagtctt caggcccgaa
     3481 gttgagtggt gccacgtatg gaacactgac atctacatca tcagggtaat agtaccggtg
     3541 gaacatcact gtctgtcctc ccttgctgat accggtggtt gcccacctgc ccctgaaaac
     3601 gggtttaaag agctcaatga tcctgtgatg atcagtagct gcctgccagg tagtcaggta
     3661 tttccagtca accggatcgg gtgtcgactc ctcgaaatat ctgtgctcaa tcctgatcag
     3721 gttggcattc aggatggaag caagctcatt ccggcctccc cgggcgccgt atccctcggt
     3781 ctcaaccaca acaggcctgt cagcaccggc ataataaagg aatacctgtt gggtaaactg
     3841 gggaccgtca ggattacggt ggtcaaccgg ttgccggagg cgtatctccc acgcctcact
     3901 gaatgaagtg tctgccttta tcctgaaaag tgtatctgcc gggaggctcg ccagacgctc
     3961 ctccagggtc atgatcttct ttgagcatcc ggtggtgagg atggctgcaa ccattaaaac
     4021 aaaaaggtta agtctgaaca ttggatttac gattttaaat tttcctttct cagcggctta
     4081 gcggcatcgt tttggctctc aaccagatct gcaactccgg ctcaaggtac ctgtgaagag
     4141 accggtaaac cttctcatgg tacccgttca gccactgaag ttcactgtca tccaggagag
     4201 agatgtcaat aagttcacga tcaatataac aaagtgttac tgtctcaaac gacaggaaac
     4261 taccaaattc gctctctgtg tcagtgcagc agaccagaag gttctcggtc cggaaaccat
     4321 agtgccccgg acggtaaaaa gccggctcat ctgaaatcac catgcccggt tccatactgg
     4381 tcttcatgtc acctgaagcg cttgtgccta ttgtctgtgg tccttcatgc acgttcagga
     4441 agctgcccac accgtggcct gtaccatgac cgtagttcat gtaattatcc cacaaaggct
     4501 tgcgggcgag aatgtcaagc tggtatcctt ttgtgccacg cgggaagcgt gccatggcaa
     4561 ggtcaatggt tcccctcaga gcaagcgtga attccttctt catccgggca tcaggctttc
     4621 caagagcaac cgttcttgtg atatcagtgg tccctccata atactgcccg cctgaatcaa
     4681 gcagaaaaat tccttccttc ttcagcctga catcagtttt catgtcaggg acgtagtgcg
     4741 gaagggctgc atgttcatta tatgcagcaa tggtactgaa cgagggacct gtgcagccag
     4801 tctgcgttga gcgcatctca tcaattttcg cggcagcgct catctcggtt atctcctccc
     4861 ttccaactga cttttcaagc cagaaaaaaa agcgggtcag aactacgccg tcatcaacca
     4921 tcacttttct caggttcttc agctcaacct cattttttac cgctttcatg cgggtgggaa
     4981 tactcaggtc ctctgcaata tccacgctgc ggggaagtgc cctgtacagt gctgccgggg
     5041 ttgacggggg cgaaagcaga acaacggacc cctcttccag atgagacaga acggaggtca
     5101 ccgtctcata gggcaacaga acaacactgt cactgtcaag tgaagccttt acctcatgtg
     5161 gaattttatc ctcatcagca aagaagatga cctgtttgag actcaccacc gcaaaagaga
     5221 gaagcagcgg tgagtacttc acatcgttgc ccctgaggtt caacagccac ataatatcat
     5281 ccgttgctgt aagcagctga taatcaacct tcattatctt cattgcctcc ctgacccttt
     5341 ccagtttctc atgcctggtc aacccggcat acttaacgtc aagttcaaag gcactgtcgc
     5401 cgggcagatc agggcgatct ttccagagag gagtgatcag gtcggctttc aggttgagag
     5461 tggcaccgga ggctttaagg gatgatatca gcagcttca
//
LOCUS       DAOPBK010000158         5467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_12791_length_5467_cov_4.747783, whole genome shotgun sequence.
ACCESSION   DAOPBK010000158 DAOPBK010000000
VERSION     DAOPBK010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12791_length_5467_cov_4.747783"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLA17_08985"
     CDS             complement(<1..430)
                     /locus_tag="PLA17_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPK85590.1"
                     /translation="MSKKTKSSSGVWQSIFAPLAIIVAFIIAYLIFTRLLGNPVNFEG
                     GNPKGHPLQGNLLGLMYKGGVIVPILITILIVLIIYCFERLITLTKIKGKARLNVFVT
                     NVKQMLAEDRIEDAIVACDKQKGSLANVLKAGLSRYRALES"
     gene            complement(546..2732)
                     /locus_tag="PLA17_08990"
     CDS             complement(546..2732)
                     /locus_tag="PLA17_08990"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK85591.1"
                     /translation="MRSLKLALLPAAVFCMVFGTGLKAQTLDEARTFTKNEQYDKAEV
                     LFQQLIKNEPTNSNIFFHYGENALLNYFADTISNSLNIAMKEAADIFEKGVAANAADP
                     LNHIGLAKVALYNGDYARAEELRLKARELLPPYKKVTKIKNPKSYAYALAKIAESYIR
                     FEQVDTSKALPFVKQALSIDPRNSEVYMIAGDIYDLVNDASKAIKNYNLAQDWDLTSP
                     AANMKIGSIYVKGRNLNAAIPYYEQAIALDQDYAPAYRELGQLYSMAGRFTDSKKYFE
                     KYLELTQGNIPAKIRYVNALFYAKEYEEVIKNVEEIFLVDQSRTYMNRIAGYSAYEEG
                     NFELAKQYMDKLFANLDADRIIKKDYIYYARILARKNQNYAKLIIDVDNDADELSKLQ
                     ARYATLKSPAKEQAKASIDALQVRVEEGRAQVKNAEAELAKSFEAYDKAIRFNEEEDL
                     NLLYEKGTIQYGARKYADAASSWSLLLDKGRDSETDFLQVGRAYYQDKAFDKADEAFN
                     KMVAKYPDNLQGYLWLANIASAKDPDSELGLAKPKFQTLLQKAAADTVKNVKEIHDAL
                     RYLGYEALQNKRYDEAKAYYNRMMNLSPDYRIKAHSSLSTMYMTMGDYPKAIEENNKI
                     LALEPGNEAAKSSIQYITQLQKSALPKAHPNEITGVISDSNGDPIPGASVRVKDTAAE
                     AWTNARGEYKFVMPEFSETLVISAKGYKSIEVPVTKRRTYNATLDK"
     gene            complement(2754..3713)
                     /locus_tag="PLA17_08995"
     CDS             complement(2754..3713)
                     /locus_tag="PLA17_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK85592.1"
                     /translation="MNKAKSLTAATAGVILLALLNLMPGCRNKGTAALKETTTSGNIR
                     IAADDSFKPIIDAELDTFTALYPYAHITPVYMPENELVAFFLNDSVKVIASSWAPTEE
                     QRKLLLDMKTVVRTTVVAYDALALVLHRSNPDSLLTYENVKDIFTGNITDWKQINSSS
                     NLGQINIVFDNVRSGNIRYFREIFSLPEELLPNFFAVNTNNEVIDYVAQTPGALGIVS
                     VNWISDEDDTESFSMLSKIKVAAISQQYLDKSSYYLPLQGHIYEKTYPFVREVNMHSR
                     ESFTGLGSGFVSFVAGEKGQRIILKSGLIPATMPIRIMQVKNK"
     gene            complement(3888..4118)
                     /locus_tag="PLA17_09000"
     CDS             complement(3888..4118)
                     /locus_tag="PLA17_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85593.1"
                     /translation="MNSSKMYDKVFAIFGTVMVFFYFGLAIFVLTTKIFNIDKAMRII
                     LSVPLFIYAIYRVFVSYEKIRDVFFSSGDSDE"
     gene            complement(4241..>5467)
                     /locus_tag="PLA17_09005"
     CDS             complement(4241..>5467)
                     /locus_tag="PLA17_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85594.1"
                     /translation="PAIVQEFQTLADKVSMAGPVTDLGAFAESYLNVFSRGYNYAFGI
                     AAAAMVISLLVYVSFNKLLPNKEKPVKASADSANTLEFEIRPLIYSIIAMAIFAFGLQ
                     FIVGWASGLAFGLFAGFVTWILTTAKKDEYARVSSLILVFVVVIFFWMSFHQNGLTLT
                     LFARDYTVKQVGPFTNLFFNLPSMLAVIGSIAGLVMLLRRNMSGTTKIIGGALFVVAM
                     VAAYLFVIKADAMNAIEPEVFQSFNPLSIVTLTFPVMGLFAWLNKKGIEPSTPKKIGI
                     GMLIASVGFVVVLIASVKLPSPASLGGSPVAEALRVTPYWLISSYLILTVAELFLSPM
                     GLSFVSKVAPSRFQGLMQAGWLLATAVGNKLLFVGTKLWDKVELWQLWTVFIVCCLLS
                     AAFIFSIMKRLENATK"
BASE COUNT         1374 a         1243 c         1269 g         1581 t
ORIGIN      
        1 cactttccag ggcacggtag cgcgagaggc ctgccttgag aacattggca agtgaaccct
       61 tctgcttgtc acatgcaacg atggcatctt caatcctgtc ttctgcaagc atctgtttca
      121 catttgtgac aaacacgttg agtcttgcct taccttttat ctttgtcaga gtgataagcc
      181 tttcaaagca ataaatgata agaacaatca gaattgtaat aagaatgggc acgataacac
      241 cacccttgta cataagaccg agcaggttac cctgcagggg gtgacccttg gggttgccac
      301 cttcaaagtt aaccgggtta cccaggaggc gggtaaagat tagataagca ataatgaatg
      361 caacaatgat tgctaggggt gcaaagatcg actgccatac accacttgac gacttggttt
      421 ttttactcat ttcagtattc tttagttggt ttaagtaaac aggtgtcaaa agtaattatt
      481 ctcaatttgt tttacaatag ccaaaatcaa ttagatcaat tttataactc attaataatt
      541 atatgtcatt tatcgagtgt ggcattataa gtccttctct ttgtcacagg tacttcaatt
      601 gacttgtagc ctttggcgct gattaccagt gtctctgaaa attcaggcat cacgaacttg
      661 tactcacccc ttgcgttggt ccatgcttcg gcagcagtgt ccttaaccct tactgatgct
      721 cccgggattg gatcgccgtt tgaatcagag ataacgccgg tgatctcgtt gggatgtgct
      781 ttcggcagtg cactcttctg gagctgtgtt atgtactgga tggatgactt tgcagcttca
      841 ttgcccggtt ccagagcaag aatcttgttg ttttcctcga tagccttagg gtaatcaccc
      901 atggtcatgt acatggtgct cagcgagctg tgagccttga tacgataatc gggcgagaga
      961 ttcatcatcc tgttgtagta cgcctttgct tcgtcatacc tcttgttctg cagagcttcg
     1021 tagccaagat aacggagggc gtcatgtatt tccttcacgt tctttaccgt gtcagcggca
     1081 gctttttgga gaagagtctg aaacttcggt tttgcaaggc caagttcaga atcgggatcc
     1141 ttcgcggagg ctatattggc cagccaaaga taaccctgga ggttgtcagg gtattttgca
     1201 accatcttgt taaaggcctc gtcagccttg tcaaaggctt tatcctggta ataagccctt
     1261 ccaacctgca ggaaatcggt ttcactgtcc cttcccttat caaggagcag tgaccaggat
     1321 gacgctgcat cggcgtactt gcgggcaccg tactgaatgg ttcccttttc atacagcagg
     1381 ttgaggtctt cctcttcatt gaatctgatt gccttgtcat atgcttcgaa tgactttgca
     1441 agctcggctt ctgcattctt aacctgtgca cggccctctt caaccctgac ctgcagggcg
     1501 tcaattgatg cctttgcctg ctcttttgct ggacttttca gcgtggcata ccttgcctga
     1561 agcttactca gttcgtcagc atcgttgtca acatctatta taagctttgc ataattctgg
     1621 ttcttcctgg caagaatcct tgcatagtat atgtagtctt tcttgatgat gcggtcagca
     1681 tcaaggttgg caaaaagctt gtccatatat tgctttgcaa gctcaaaatt gccctcttca
     1741 tatgctgagt agccggcaat tcggttcata tatgtccttg actggtcaac caggaagatc
     1801 tcctcaacat ttttgatcac ctcctcatat tcttttgcat agaacagtgc attgacatac
     1861 cttatcttgg cggggatgtt tccctgggtc agttcgaggt atttctcaaa gtactttttg
     1921 gagtcagtga atcttccggc catggagtaa agctgcccca gctccctgta tgcgggtgca
     1981 taatcctggt ccagggctat ggcttgctca tagtagggga tggctgcatt gaggttgcgt
     2041 cccttcacat agatacttcc gattttcatg tttgctgcag gggatgtcag gtcccagtcc
     2101 tgggcaaggt tatagttctt gatagccttt gaggcatcat tgaccagatc atagatgtcg
     2161 cctgcgatca tatagacctc gctgtttctc ggatcaattg agagtgcctg ctttacgaag
     2221 ggaagtgcct tgctggtgtc aacctgctca aaccggatgt atgattcggc aatcttggcc
     2281 agagcatagg cgtaactttt cggattcttg atcttggtaa ccttcttgta tggaggaagg
     2341 agctccctgg ctttcagcct cagttcctca gccctggcgt aatcgccgtt gtacagggca
     2401 accttggcaa gtcctatatg gttcagcgga tcagcagcgt tggcggcaac acccttttcg
     2461 aagatgtcgg cagcttcctt catcgcaata ttcagcgagt tggagattgt gtcagcaaag
     2521 tagttcaaca gtgcgttttc tccgtaatgg aaaaagatat tgctgtttgt aggctcattt
     2581 ttaatgagtt gctggaacag aacctctgcc ttgtcatact gttcgttctt ggtgaaagtc
     2641 cttgcctcat cgagggtctg agccttgagc cccgtgccaa agaccatgca aaataccgct
     2701 gcgggcagca acgcgagttt taagcttctc ataatcatcg ttatcaagtt tagttactta
     2761 ttttttacct gcataattct aatcggcatc gttgcaggta taagacccga tttaagaatt
     2821 attctttgtc ctttttctcc tgcaacaaaa gatacaaatc ccgagccaag cccggtaaat
     2881 gattcccttg agtgcatatt tacctcacgc acaaatggat acgttttctc ataaatatgt
     2941 ccctgcaagg gcagatagta tgatgattta tcaaggtatt gctgtgatat ggctgctacc
     3001 tttatcttgc tcagcatact aaagctctcc gtgtcatcct cgtcacttat ccagttgaca
     3061 ctgacgatgc ccagagctcc gggtgtttgt gctacataat caattacctc attattcgta
     3121 ttaactgcga aaaagttggg cagcagttct tcgggaaggg agaatatttc cctgaaatat
     3181 cttatgttgc ctgacctgac attatcaaac acaatgttta tctggccgag gtttgatgag
     3241 gagttgatct gtttccagtc agtaatgttg ccggtgaaaa tatcctttac attctcatag
     3301 gtcaggagtg aatcggggtt gctgcggtgg aggactaaag ccagggcgtc atatgccaca
     3361 acggttgtac gaaccacagt cttcatatca agaagcagct ttcgctgttc ttccgttggt
     3421 gcccaggatg aggctatcac ttttaccgaa tcattcagga agaaggccac cagttcattc
     3481 tccggcatat agacgggagt gatatgagca taggggtaga gggccgtgaa agtatcaagt
     3541 tcagcgtcta tgatgggctt gaatgaatca tcagctgcaa ttctgatgtt gcctgacgtg
     3601 gtagtctctt tgagagctgc agttcccttg tttctgcatc caggcatcag gttaagcaat
     3661 gccaggagga tcactccggc ggttgctgcc gtgagggatt ttgctttgtt catccttcct
     3721 ttacttatca acttgctatg ggccacaata ttaataaaaa tatgtttata aaacgggttt
     3781 tatgatatta aaaaaatcac ttcttcttca tttagtgttt tcaaaatctg tgccatcaat
     3841 gaaaaataac tgatttttaa tactattatc ccctcttttt ctttaaatta ttcatcacta
     3901 tccccggaac tgaaaaaaac atccctgatc ttttcatagg atacaaacac cctgtagatg
     3961 gcatagatga agagcgggac tgacagtatg attctcatgg ctttatctat gttgaaaatc
     4021 ttcgtggtaa gtacgaagat cgccaggccg aaataaaaga acaccattac tgttccgaag
     4081 atcgcgaaga ccttgtcata cattttactg ctgttcattc tgatcaggtt aacctgacaa
     4141 atatattatt aatatccttt ttacatgcag acattaatta tttattggca aaaaaagaga
     4201 gccgcgttag cagctctccc ttatgattac agatgatccg ttattttgta gcgttttcca
     4261 gtctcttcat tattgagaaa atgaatgctg ctgacagcag gcaacagacg atgaacacgg
     4321 tccacagttg ccacagttca accttgtccc aaagttttgt tcccacgaag aggagcttat
     4381 tgccgacagc agttgcaagg agccaccctg cctgcatgag tccctggaat cttgagggag
     4441 ccacctttga aacgaatgag aggcccatgg ggctaaggaa gagttctgcc actgtcagga
     4501 taaggtagct gctgatgagc cagtatgggg tcaccctgag agcctcggca acaggagacc
     4561 ctccaagtga tgccggtgac ggcagtttga ctgacgcaat aaggacaacc acgaaaccca
     4621 cggatgcgat cagcataccg ataccaatct ttttgggtgt tgaaggctcg atgccctttt
     4681 tgttaagcca ggcgaagaga cccattacgg ggaaggtaag ggtcacgatg gatagcggat
     4741 tgaatgactg gaacacctca ggttcgattg cattcatggc atctgccttt atcacgaaaa
     4801 gatatgcggc aaccattgcg accacaaaaa gggcgcctcc gataatcttt gtggtgccac
     4861 tcatgttcct cctgagcagc ataaccaggc cggcaattga acctataacg gcaagcatgg
     4921 agggcaggtt gaagaagaga ttagtaaatg gtccaacctg cttcacggtg tagtccctgg
     4981 caaagagcgt cagggtaagg ccgttctggt ggaatgacat ccagaagaag ataaccacca
     5041 caaataccag tatcagggaa gagacacggg catactcatc cttcttggcc gttgtcagga
     5101 tccatgtaac gaatccggcg aacagtccga aagcaaggcc tgatgcccat cctacgatga
     5161 actgaagccc aaatgcgaag attgccatcg cgatgatgga ataaataagg ggcctgattt
     5221 caaactcaag ggtgttggcg gagtcagccg atgccttgac gggcttttcc ttgttgggaa
     5281 gaagcttgtt gaagcttaca tagacgagca gggatatcac cattgctgct gctgcaatac
     5341 cgaaagcata gttgtaacct cttgagaaga cattgagata gctttcagcg aatgcaccaa
     5401 ggtcagtgac cggtcctgcc atacttacct tgtcagccag ggtctggaac tcctgcacta
     5461 ttgcggg
//
LOCUS       DAOPBK010000159         5376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13029_length_5376_cov_5.085322, whole genome shotgun sequence.
ACCESSION   DAOPBK010000159 DAOPBK010000000
VERSION     DAOPBK010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13029_length_5376_cov_5.085322"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..549)
                     /locus_tag="PLA17_09010"
     CDS             complement(40..549)
                     /locus_tag="PLA17_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPK85595.1"
                     /translation="MRSLTRGARQSMMSNLMKSLLLATLFTTLLPLSLTAQRGLGVEE
                     LMNDGRPRYMADRLVVLQEPEIDTLVSRHILMNASKNGLDGWRIQIYRGGHRTANEDS
                     NKICAQFMDEYPDIPAYRTFDRPNWFKVKVGDFRTREEAAKVFFEIQSKYPDAYLIRD
                     VIAFKTQVK"
     gene            complement(624..1379)
                     /locus_tag="PLA17_09015"
     CDS             complement(624..1379)
                     /locus_tag="PLA17_09015"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK85596.1"
                     /translation="MSGHNKWSTIKRKKGAADAKRGKIFTKIIKEITISVREGGTDPA
                     GNARLRLAIQNAKGVNMPKDNIERAIKKAGGSDAEAYNEITYEGYGPNGIAVFVEGLT
                     DNTNRTLSSIRFIFSKYNGTLGPNGSLTFLFDRKGVFTINPEDVGSRDMESLELELID
                     AGAEDIETDEDIITVTCAMDDFGNLNRKLEELEIEPDEAKLQRVPKDTKTLDVESARK
                     VMRLIEALEDDDDVQAVYHNLEITDELARALEE"
     gene            1505..2956
                     /locus_tag="PLA17_09020"
     CDS             1505..2956
                     /locus_tag="PLA17_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK85597.1"
                     /translation="MPKKYYIETYGCQMNVADSEVVASILSDAGYSPSVSIDDADIIL
                     VNTCSIRENAEQRVWGRLDLFRAQKKKRPQVIVGVLGCMAERLKEKLLESGKMVDIVA
                     GPDAYRDLPMLVETAAAGNPAVNVLLSREETYADISPVRLGSNGVSAFISIMRGCNNM
                     CAYCVVPYVRGAERSRDPESIIREAKEAFDMGFREVTLLGQNVNSYAWNGAVPEDEVT
                     PAGLLKKMSDQHSGHRFSNLVNGLTAPERYPLTGKQEPVNFAGLLEMVASVHPELRIR
                     YSTSHPKDLSDDVLEVMAGHNNICRHIHLPVQSGSSSVLKRMNRKYTREWYLGRVAAI
                     NRILPGCTLSTDIIAGFCGETEEEHQETLSLMREAGFDFAYMFKYSERPGTKASRKMK
                     DDVPDEVKTRRLNEIIALQNEISAVSKKADTGQTFEVLVEGPAKKPAGAMTGRTSGNK
                     VVVFRASEVQPGDYVNVTVTRSTSATLIGNLMT"
     gene            3186..4103
                     /locus_tag="PLA17_09025"
     CDS             3186..4103
                     /locus_tag="PLA17_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85598.1"
                     /translation="MDDRIHVSSIVLHRGEEPPLSGTKGIVNLFFPSCNMQCIYCQNW
                     EISRRGTQGIVMSLDEVCDAIADLLPLSENNLGFVSPTHFLLQMVSIVKELRSRGLSP
                     TIVYNTNGYELPETIRSLEGIVNVWLPDFKYSDDALAESLSLAPDYSQYALRSVKEMM
                     RQVGTTLHTDDRGIARRGIIIRHLVLPGFVNNSIGVLRLISENLSPNLHISLMSQYYP
                     PDNPAFIYLKSKFSHDESQEGYPLLRVRNRTSDIEHGDSLYHTVTRKEYKAVTGSFYS
                     LGFTHGWLQDPISHKNYRPDFSKDNPFGR"
     gene            complement(4125..>5376)
                     /locus_tag="PLA17_09030"
     CDS             complement(4125..>5376)
                     /locus_tag="PLA17_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPK85599.1"
                     /translation="LALIATVTPANATNKSVTWSIINGTGQATINSSGLVTAVADGTV
                     TARATANDGSGVYGQLVITISGQTVLVAKMYVAAAKGEPVISTPKGTLQLSVMVQPDN
                     ATNKTVTWSVINVTGEATINSSGLVTAIADGTVVARATANDGSGVFADITISIINQIV
                     LVTGITVTGEGGLTTITTDDGTLQLLASIVPSNASNKSVTWSMVKGNGHARLSEEGLL
                     TARTNGKVVVRATSNDGSKVYGEIEITITNQIVSVSSIKVKVKKKASNTTTVNGELAL
                     AAEIFPEDASEKRVSWSVINGTGTAVISEDGILRGVTPGEVIVVATSLDGSGVAGELS
                     ITINLVESIKIRQTRNEVIIQVPEYLLPAKASLHSLYGSHIQSKVIDSTECIFNISEL
                     FPGIYVVSVYNSIVQDAAKIMIAY"
BASE COUNT         1280 a         1347 c         1323 g         1426 t
ORIGIN      
        1 ccagatctgt ccgaggttag tttcgcgttg ccgcttgttt tacttaacct gggttttgaa
       61 agcgatcaca tccctgatca ggtacgcgtc agggtacttg ctctgtatct cgaagaatac
      121 ctttgcggct tcctcgcggg tgcggaaatc acccactttc accttaaacc agttgggacg
      181 gtcgaaggtg cggtatgcgg gaatgtcagg atattcatcc atgaattgtg cgcagatctt
      241 gttggagtct tcgtttgccg ttcggtggcc gccgcggtag atctgaatgc gccatccgtc
      301 aaggccgttt ttcgaggcat tcataaggat atgccggctg acaagggtat cgatttcagg
      361 ctcctgtaat actacaagac gatctgccat gtagcgcgga cgtccatcat tcataagctc
      421 ctcaacaccc aggcctctct gcgcggtcag tgacagcggc aggagggtgg taaacagcgt
      481 ggcaagcaac agtgatttca tcaggttgct catcattgat tgtctcgctc ctcttgtcag
      541 tgatctcatg cggcaaatat acagctgttt ttccgggttg tcctgtgcaa aaattattcc
      601 agaatcttga gagggggctc cctttactcc tccagtgctc tggccagctc gtcggttatc
      661 tcaaggttat gatatacagc ctgcacatca tcgtcatcct caagagcctc aatgagcctc
      721 atgaccttgc gggccgattc cacgtcaagt gtttttgtat ccttcgggac cctctggagc
      781 ttggcctcat ccggttcgat ctcaagttct tccagtttgc ggttaagatt tccgaagtca
      841 tccatggcgc atgtgactgt tattatatct tcatcagtct caatatcttc agcgcctgca
      901 tcgataagtt cgagctcaag gctttccata tccctggagc cgacatcttc gggattgatt
      961 gtaaagacac ccttccggtc aaacaggaag gtcagggaac cgttcggtcc gagagtcccg
     1021 ttatacttgc tgaagatgaa ccttatgctt gaaagagtgc ggtttgtgtt gtcagtaagg
     1081 ccttcgacaa acacggcgat gccgttcggg ccatagccct cgtaggtaat ctcattgtat
     1141 gcctcggcat ctgagccgcc ggcttttttg atagctctct cgatgttgtc cttgggcatg
     1201 ttgacaccct tggcgttttg tattgccagc cgcaggcgtg cgttgccggc agggtcagtg
     1261 ccaccctcac gtactgagat cgttatttcc tttatgatct tggtaaagat ctttcctctc
     1321 tttgcatcag ccgctccctt tttgcgtttg atggttgacc atttattgtg acctgacata
     1381 tggataaatt cttacaaaac acaaaaatat acaattcctg aaatctgcaa tcacgatacc
     1441 ttgtgaggtc tcctctgacc tgataagaac gaccttcaga ctttttccta cctttgcttc
     1501 ggttatgccc aaaaaatact atatagagac ttatggctgc cagatgaatg ttgctgacag
     1561 cgaggttgtt gcatcaattc tttcagatgc cggatattca ccatccgtaa gtattgacga
     1621 tgctgacatc atactcgtca acacctgctc tatccgggag aatgccgaac aacgggtatg
     1681 gggccgcctt gacctgttcc gggcacaaaa aaagaaacgc ccgcaggtta ttgtgggcgt
     1741 gctgggatgc atggccgaac ggctgaagga gaagctcctc gagagcggca agatggtgga
     1801 tattgttgca ggaccggatg cctaccgtga tctcccgatg ctggttgaga ccgctgccgc
     1861 aggtaaccct gctgtcaacg tgctgctttc ccgcgaggag acttatgcag acatctcgcc
     1921 ggtacgcctc gggagcaacg gtgtttccgc gtttatatca atcatgcgcg gctgcaacaa
     1981 catgtgcgca tattgcgtgg tgccctatgt gcgcggtgca gagcgaagcc gtgatccgga
     2041 gtcgattatt cgggaagcga aggaggcttt tgatatgggc ttccgcgaag taaccctgct
     2101 ggggcagaat gtgaattcct atgcatggaa tggagcagta ccggaagatg aggtcacccc
     2161 tgccgggctc ctgaaaaaaa tgtcggacca gcattccgga caccgtttta gcaaccttgt
     2221 gaatggcctg actgctcctg aaaggtatcc gttgaccgga aaacaggaac cggtaaactt
     2281 tgcaggactt cttgagatgg tggcttcagt gcacccggag ctgcgcatcc gctattccac
     2341 ctcccaccca aaggatctgt cggacgatgt gcttgaggtc atggctggtc acaacaatat
     2401 ctgccggcac attcaccttc cggtacagag cggcagcagc agtgtgctga agcgtatgaa
     2461 caggaaatat acacgtgagt ggtaccttgg cagagttgct gccattaacc ggattctgcc
     2521 aggatgtacg ctttccactg atatcatcgc aggtttctgt ggcgagactg aggaagagca
     2581 tcaggagacc ctcagcctca tgcgtgaggc aggattcgac tttgcctata tgtttaaata
     2641 ctcggaaagg cccggcacaa aggcctcgcg gaagatgaaa gacgatgtgc ctgatgaggt
     2701 caaaacccgc aggcttaatg aaatcatcgc tctgcagaat gaaatctctg cagtcagtaa
     2761 gaaggcggat acaggacaaa cctttgaggt cctcgttgaa ggtcccgcga agaaacccgc
     2821 cggtgccatg acggggagga catcaggaaa caaggtggtt gtattccggg cgtcggaggt
     2881 acagccgggt gactatgtta acgttactgt cacacgttcc acctctgcca cgctgatagg
     2941 gaacctgatg acatgacagc aggcgagtag ccgcctctgg ctggtaagaa ccgaaccgct
     3001 tatatgcggt gagcccggcg aagccaactc cctatagcaa aacaaaccgg gaattgtaag
     3061 acccggacat cgcaaattga aaactgagaa ttgaaaaaac gcaaatcccg attaccttga
     3121 aagcatgtaa tctctgcccc cgccgctgca ataccgaccg ctcccggact gtcggatggt
     3181 gccgcatgga cgacaggatt catgtttcct caatagttct gcatcgcggc gaggagccgc
     3241 ccctttccgg aacaaaaggg attgtcaacc tgtttttccc atcgtgcaac atgcagtgca
     3301 tctattgcca gaattgggag atatcgcgcc gtggaacaca gggaatagtg atgagccttg
     3361 acgaggtgtg tgacgcaatt gctgacctcc ttcctctttc ggaaaataac ctcgggttcg
     3421 tctcccccac acacttcctg cttcagatgg tatcaatagt gaaagagctg aggagccggg
     3481 gactgtcgcc aacaatagtc tataatacaa atggttatga gctgccagaa acaatccgct
     3541 cactcgaagg catcgtgaat gtctggctgc ccgacttcaa atattctgac gatgctctgg
     3601 ctgaatcact gtctcttgcg ccggactaca gtcaatatgc attgcggtct gtaaaagaga
     3661 tgatgcgcca ggtgggtaca accttgcata ctgatgaccg gggcattgcc cgtcgcggaa
     3721 taattatccg tcatctggtt ctgcccgggt ttgtgaataa cagcattgga gttctgagat
     3781 tgatttctga aaacctgtcg cccaacctgc atatctcact gatgtcccag tattatccac
     3841 ctgacaaccc tgcctttata tacctcaaat ccaaattctc acacgacgaa tcacaagaag
     3901 gatatccctt attgcgagtc cgaaaccgaa catcggacat cgaacatggc gattcgcttt
     3961 accatactgt caccaggaaa gagtataagg ctgtcaccgg ctcattttat tccctcggtt
     4021 tcacccacgg ctggctccag gaccccataa gccataaaaa ctaccgtccg gatttctcga
     4081 aggataatcc tttcgggagg tagaggacca ggagaaaagg ttatttaata agcaatcatg
     4141 atttttgcag catcctgcac tatagaatta taaactgaga ccacgtatat tcccgggaat
     4201 aattctgata tattaaagat gcactcggtt gagtcaatta cctttgactg aatgtgagag
     4261 ccatagagac tatgcagact tgcttttgca ggaagcagat attcaggtac ctgaattatt
     4321 acctcatttc ttgtttgcct gatcttgatc gattccacaa gattaattgt aatcgaaagc
     4381 tcacctgcga cgccggatcc atcaagtgat gttgctacaa caatcacttc gccgggtgtg
     4441 acacctctaa gtattccatc ttcgcttata actgcagttc cggtaccatt tatcactgac
     4501 catgagactc ttttctcgga agcatcttcg gggaagattt cagctgcaag cgcaagctcg
     4561 ccattcactg ttgttgtgtt ggatgctttt ttctttacct tgactttaat ggaggagaca
     4621 gaaacaatct gatttgtaat tgtgatttca atctctccat ataccttgct gccatcgtta
     4681 gatgtagccc tgacaactac tttcccgttt gtcctggcgg ttaacagacc ctcctcactc
     4741 aacctggcat gaccgtttcc cttgaccatg gaccatgtca ctgatttgtt cgaggcattt
     4801 gaaggaacta ttgaagctaa aagttgcaat gtgccgtcgt cagtagtgat ggttgtgagg
     4861 cctccctctc ctgtcacagt aatgcctgtc acaagtacaa tctggttgat tatcgagatt
     4921 gtaatatctg cgaatactcc tgatccgtca tttgcggttg ctcttgccac caccgtacca
     4981 tctgcaattg cggttaccag tccggatgaa ttgattgtgg cctcgcctgt tacattgatt
     5041 actgaccagg ttacggtttt gtttgtagca ttatcaggtt gtaccatcac agaaagttgc
     5101 aatgttcctt tgggagttga gatgactggt tctcctttgg ctgcagctac atacattttg
     5161 gcaaccagga cagtctgacc gctgatggta ataaccagct gaccatacac accactgccg
     5221 tcgttagctg ttgcacgggc tgtaacagta ccgtcggcaa ctgctgttac aagtccggag
     5281 gagttgatag tcgcctgacc cgtgccgttg atgatgctcc aggtaacact cttgttagtg
     5341 gcatttgccg gggtgactgt tgctatcagg gctaat
//
LOCUS       DAOPBK010000160         5371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13043_length_5371_cov_4.407261, whole genome shotgun sequence.
ACCESSION   DAOPBK010000160 DAOPBK010000000
VERSION     DAOPBK010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13043_length_5371_cov_4.407261"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLA17_09035"
     CDS             <1..621
                     /locus_tag="PLA17_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPK85600.1"
                     /translation="LNMELISAIIGQVIGVGVIALIIVFQQEIRRFLLAIGNRYMSRS
                     RFSFDRYFPSGEVDQTTNQIVEEIVHAVESMAQSRTGALIAVGRTSLLDIYSDGGELL
                     NALVTNELLKTIFYKGSPLHDGAVLIQNRKILAARCPLPSTDQTNLPARFGMRHRAGI
                     GLTEHTDALVIIVSEERGRISVADAGKIQEDLTPNELRQVLLSIRI"
     gene            complement(691..1110)
                     /locus_tag="PLA17_09040"
     CDS             complement(691..1110)
                     /locus_tag="PLA17_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85601.1"
                     /translation="MSKTSLILAFLIMLLLSCDEGFLTDCDKCYTDLDFSIKLNIVFR
                     NPDRVPVNPIVTIYEGNISDSIILSRVHIADPFTYMYYPAVLYKDYSATLEFIYDSQK
                     YITTATACPRIRYDETTCEEPCYYVYDNVLDLRLRYD"
     gene            complement(1103..2506)
                     /locus_tag="PLA17_09045"
     CDS             complement(1103..2506)
                     /locus_tag="PLA17_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPK85602.1"
                     /translation="MGRIREILEVYNDRTVYVDTSEYVHGFEGADDINLQTAASTGAC
                     SEIIRLVSRGADVNTHFGFIATPLHYAVNSGKWEAVEILLLLGAKPDAKDTYGNTPLI
                     VAVRADQQDIAEKLIRYGASINNADRQRSSPLHHSTVFQNFSMTDMLLYYDSPTELFD
                     MEGNTPLMLGVCFGYHEIADLLLQSGADPNAADSRGFTPLMAASQDGDTLMMSILAEA
                     GANLYALNNDGIDALGCAVIGSQKEAAEFLLRHGNRWDHTNGTIGDPVKLANACGESH
                     ILKLMNDHGLKSKRVFNFNQLSFSAGVWYTPHYKLAGGSLSVSEPGLGAGITLGAAVD
                     PGNQRVLVDGDDGTIYQYNIRSNLIYAGLFREFPLTSKLNELRVSVVPVLNFGYMFHS
                     LYAGTEEKPDDKLYFIPEADIRFSWKSLSLNPGLAIMKTPFRKIGPLWFTFKVTCDLK
                     RPQNKIRGKRIRIYNYE"
     gene            2768..4063
                     /locus_tag="PLA17_09050"
     CDS             2768..4063
                     /locus_tag="PLA17_09050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPK85603.1"
                     /translation="MRLLLIVFLVLFIFAPKVGTERVKMIDGQYVPVNLRINNSLSDN
                     DMFVKAEAGVEWFMKTWDIKGASVAVAKEGKLLYARGFGYASLEDSIPVEPYHRFRIA
                     SVSKLVTAVAIMKLQEEGKLSVYEKVFGPGAILDDTIFAHPRDKRAFDITVGHLLTHK
                     GGWSQRYGDHMFMPEVISRTLNVPMPVDLRTIIRFALTKKLHFTPGAGQSYSNLGYAI
                     LGLVIEEASGMDYESYCRKQVLEPLGIYDMALGKNLPENALPLEVSYYDVENAPLRPS
                     IYGTGEMVPASRGGNDIETLGAAGAWVATAPDLMRLLLAIDGFEYPKDILSQGSIEFM
                     TDAKIGYAPVGWRTTLSNGVWWRTGSFSGTSAMMKRMPDGTAWVVLMNSSGWNGPELT
                     GDIDQMMAKFLLRVKQWPETDLFSYYTPVQLKFAKVSEP"
     gene            4082..4804
                     /locus_tag="PLA17_09055"
     CDS             4082..4804
                     /locus_tag="PLA17_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPK85604.1"
                     /translation="MCFTVNVNIVREELEARYGSELIDHDKYRPSYYYHAYSLPSMPV
                     VCSDRGAVIRLFRWGLIPSWVTDEGEANEIMLKTFNARSETISSKPAFRDSYASRRCL
                     VPVCGFFEWQHHGGRKIPWYITLKGETFFSLAGIWDSWSMKGGMTLNTFSVVTTRANE
                     LMEEIHNSKKRMPVILPASAERMWLSEGADERTLAALMEPVATDTLSAHTVSPLITNT
                     RADRNRPELIMPFSYPVQGRLF"
     assembly_gap    4813..4822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4823..>5371)
                     /locus_tag="PLA17_09060"
     CDS             complement(<4823..>5371)
                     /locus_tag="PLA17_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85605.1"
                     /translation="AKSDQFQGISPEEMVNIIKNIADKPLDLDPKEYEEMVREEMVRS
                     GLLNERNLIDQQKMAEEAGIEDIIATPEEVKPQVTEKKEKPQEKGTFRGPTRIFYDLA
                     GRHHVYLPIPIYKCEGAGQVMLAIVVDRSGNVLRAELASKLSTTRDQCLTETAVEHAL
                     KTKFNADQSAPEKQGGYLTFVFV"
BASE COUNT         1347 a         1296 c         1349 g         1369 t
ORIGIN      
        1 ctcaatatgg agcttatcag cgccataata ggacaggtga taggggtagg ggtcatcgcg
       61 cttatcatag tgtttcagca ggagatcagg aggtttcttc tggctatcgg gaacaggtat
      121 atgagccgga gcaggttctc atttgaccgt tatttcccgt ctggggaggt ggatcagacc
      181 acaaaccaga tagttgagga gattgttcat gccgttgaat caatggcaca atcgcgcaca
      241 ggcgcactta ttgccgtggg tcgtaccagc ctcctggaca tctactctga cgggggtgag
      301 ttgctcaatg ccctcgtgac caacgagctc ctgaagacaa ttttttacaa gggttcaccg
      361 cttcatgacg gggcagtgct tatacagaac aggaagattc ttgctgcccg ctgtccgttg
      421 ccatctactg accagacaaa ccttccggca cggtttggta tgcggcaccg ggcaggaatc
      481 ggccttacgg agcatactga tgccctggta atcatcgtat ctgaagagag gggacgaata
      541 tccgttgccg atgcaggaaa gattcaggaa gatttgacac ccaatgaact caggcaggtg
      601 ctgctgagca tcagaatctg atacacctga gcccggagcc tgtttgcagt cagaacatat
      661 gcggaggtaa cagccctccg aagaacatta tcagtcatac ctgagcctca ggtcaagaac
      721 attgtcataa acatagtaac agggttcttc acaggtcgtc tcgtcatatc gtatcctggg
      781 gcaggctgtg gcagtggtga tgtatttctg actgtcatag atgaactcca gggtggctga
      841 gtaatctttg taaagtacgg ccggataata catgtaggtg aacggatctg caatatggac
      901 cctcgataag atgatactgt cgctgatatt cccttcataa attgtaacga tgggatttac
      961 cggaaccctg tcaggatttc tgaaaacaat attgagctta atactgaaat caaggtcagt
     1021 gtagcacttg tcacaatcag tcaggaagcc ttcgtcacat gaaaggagaa gcattatcag
     1081 gaatgccagt ataaggctgg ttttactcat aattatagat tctgatacgt ttgcccctga
     1141 ttttgttctg tgggcgttta aggtcacagg tcaccttaaa ggtgaaccat aatggtccga
     1201 tcttgcgaaa aggtgtcttc atgattgcca gacccggatt caggctcagg cttttccagc
     1261 tgaaccggat atctgcctca ggaataaaat ataatttgtc atcaggcttt tcttccgttc
     1321 cggcgtaaag tgaatgaaac atgtatccaa aattcagaac aggaacaaca ctgacccgca
     1381 actcgtttaa cttactggta aggggaaatt ccctgaacag gccggcgtag atgaggtttg
     1441 atcttatgtt atactggtaa attgtaccat cgtcaccatc caccagcaca cgctgattgc
     1501 cagggtcaac agcagcaccc agagttattc cggctccaag gccgggttca gaaacagaga
     1561 gtgaccctcc ggcaagcttg taatgtgggg tataccatac tccggctgaa aatgaaagct
     1621 ggttgaagtt aaacacccgc tttgacttca gcccgtggtc attcatcagc ttcagaatat
     1681 gtgactcacc acaagcgttt gccagtttca cggggtcacc aatcgtgccg ttggtgtggt
     1741 cccagcgatt gccatgcctg agcagaaatt ctgcagcctc tttctgtgat cctatcactg
     1801 cgcagccgag ggcatcaatt ccgtcattgt tcagggcata caggttggcc cccgcctctg
     1861 caagtatgct catcataagt gtgtctccgt cctggcttgc tgccatcagc ggcgtaaaac
     1921 cccggctgtc ggcagcattc ggatctgcgc ctgattgcag cagaaggtca gcgatttcat
     1981 ggtagccaaa acatactccg agcatcaggg gagtattacc ttccatgtca aacagctcag
     2041 tgggagagtc gtaataaaga agcatgtctg tcatggaaaa gttctgaaac actgtagaat
     2101 ggtgcaatgg cgaagagcgt tgcctgtctg cattgttgat cgaagcacca taccggatga
     2161 gtttttcggc aatatcctgt tgatcagccc ttacagctac aataagcggt gtgtttccgt
     2221 aggtatcctt tgcatccggt ttggctccaa gaagcagcag tatctccacg gcctcccatt
     2281 ttcccgagtt gaccgcgtag tggagaggag tggcaataaa cccgaagtga gtatttacat
     2341 ctgcacctct tgacaccagg cgtatgatct cactgcaggc acccgtcgat gcagcagtct
     2401 gcaggtttat atcatctgca ccttcaaacc catgaacata ttcagaggtg tcaacatata
     2461 ccgttcgatc attgtaaacc tccaggattt ccctgatcct tcccattatc aactcagttt
     2521 ccagactatc agtctggcca ctcaggtcta aaaagcagat cagcaaaaaa gccggaaata
     2581 ttaactttct taaggaatgc ctcatgcata caaatttagt ctcagtttat gacccgtcca
     2641 attttgttga tatttttttc tcctggttaa aaaaaatgtc tcccgtaggg gttaaaagca
     2701 ggataatagc ctatttttgt cgcagttttt tgagaccttc attcttatga ctctgcctgt
     2761 aaaaccaatg cgacttcttt taatagtctt tttggtgctc tttatctttg caccgaaggt
     2821 tggtacggaa cgggtgaaaa tgattgacgg ccaatatgtc cctgttaatc tcaggataaa
     2881 taactccctg tctgataatg acatgtttgt aaaagcggaa gcaggggtgg aatggttcat
     2941 gaagacctgg gacataaagg gtgcatcggt tgctgtggca aaggagggta agctgctgta
     3001 tgcccggggt tttggctacg cttcactgga agattcaatt cctgtagagc catatcaccg
     3061 ttttcgtatt gccagcgtct ccaaactggt aaccgcggtg gcaatcatga aactgcagga
     3121 ggagggcaaa ctttcagtgt acgaaaaggt gttcggtccc ggtgcaatac ttgatgacac
     3181 tatttttgcc catcccaggg acaaacgtgc atttgacatc actgtcggac atcttcttac
     3241 tcacaaagga gggtggtcac aacggtatgg tgatcatatg tttatgcctg aggtaatatc
     3301 ccgcacgctt aatgtgccga tgcctgtgga tctcaggacc ataataaggt tcgcactgac
     3361 aaaaaaactg catttcactc ccggggcagg acagtcatac agcaatctcg gatatgccat
     3421 cctggggctc gttatcgaag aggcctcggg catggattat gaatcctatt gcaggaagca
     3481 ggtgcttgaa ccactgggta tttacgacat ggcactcggg aaaaacctgc ctgagaatgc
     3541 tctgcctctt gaagtctcct actatgatgt agagaatgcg cccctcaggc cttcgatata
     3601 cgggactggc gagatggtac cggccagcag gggaggaaat gacatagaga ctctcggtgc
     3661 agccggtgca tgggtggcca cagcacctga tcttatgaga ctgctccttg ctatagatgg
     3721 ctttgaatac ccgaaagaca tcctctcgca gggaagcata gagttcatga cggatgcaaa
     3781 aatcggatac gcccctgtag gctggagaac cacactctcc aacggtgtgt ggtggcgtac
     3841 cggatcattc tccggcacct cggcaatgat gaagaggatg cctgacggta ccgcatgggt
     3901 ggtgcttatg aacagcagcg gatggaacgg tcccgagctg acaggcgaca tagaccagat
     3961 gatggctaaa ttcctgctga gggtcaaaca gtggcctgaa actgacctgt tcagctatta
     4021 tacaccggta cagctgaaat ttgcaaaggt ctctgaaccc tgatcaggaa tcaacacaac
     4081 gatgtgcttt accgttaatg tcaatatcgt cagggaagag ctcgaagcac ggtatggcag
     4141 tgaactcata gaccacgaca agtacaggcc gagttactac taccatgcct acagcctccc
     4201 ttcaatgcct gtggtctgtt ctgaccgggg cgccgtgatc aggcttttca ggtggggact
     4261 cataccatca tgggttacag atgagggtga agccaatgag attatgctca aaacattcaa
     4321 tgcccgttcc gagacaatct cctccaagcc tgctttcagg gattcgtatg catcgcgccg
     4381 ctgtctcgtt ccggtatgcg gattctttga atggcaacat cacggcggca gaaagatacc
     4441 gtggtatata acccttaagg gagagacttt tttctcactg gcaggtatct gggactcatg
     4501 gagcatgaag ggcggaatga ccctgaatac attttccgtt gtaaccaccc gtgccaatga
     4561 gctcatggaa gagattcaca attcaaaaaa gaggatgccg gttatccttc ccgcttcagc
     4621 ggaacggatg tggctcagtg aaggggcgga tgaaaggacg ctggcagcgc tcatggagcc
     4681 cgtggccacg gatacgctct cagcccatac agtcagcccc ctgataacca acacaagggc
     4741 tgacagaaac aggccggaac ttatcatgcc tttcagttat ccggtacagg gaaggctttt
     4801 ctgatcagat tannnnnnnn nnacaaacac gaaggtgaga tagcctccct gcttttcggg
     4861 agcgctctgg tcagcgttga actttgtttt gagtgcatgc tccacggcag tctcggtgag
     4921 gcactgatcc ctggtggtgg aaagctttga tgcgagctcg gccctgagta cattaccgct
     4981 gcggtcaacc acaatggcga gcattacctg acctgcgccc tcgcacttgt agatgggtat
     5041 agggaggtag acatggtggc ggccggcaag atcataaaaa atccttgtgg ggccacggaa
     5101 agttcccttt tcctgaggtt tctccttctt ctcggtaacc tggggcttta cctcctctgg
     5161 cgtggcaata atatcttcga tccctgcctc ctcagccatc ttttgctggt caatgaggtt
     5221 cctttcgttc agaagaccgc tcctgaccat ctcctccctg accatctcct catactcttt
     5281 gggatcaagg tcaagcggct tgtctgcaat attcttgatt atgttcacca tctcctccgg
     5341 gcttatgccc tggaactggt cgctcttcgc a
//
LOCUS       DAOPBK010000161         5343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13111_length_5343_cov_5.647693, whole genome shotgun sequence.
ACCESSION   DAOPBK010000161 DAOPBK010000000
VERSION     DAOPBK010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13111_length_5343_cov_5.647693"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /gene="rplJ"
                     /locus_tag="PLA17_09065"
     CDS             complement(<1..186)
                     /gene="rplJ"
                     /locus_tag="PLA17_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPK85606.1"
                     /translation="MRREEKADIINSLAATLQEYSHFYLTDTAELNAADTSDLRRKCF
                     ENQIKLVVVKNTLLKRAL"
     gene            complement(210..905)
                     /gene="rplA"
                     /locus_tag="PLA17_09070"
     CDS             complement(210..905)
                     /gene="rplA"
                     /locus_tag="PLA17_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPK85607.1"
                     /translation="MAKLTRNQKFALAKIEHGKLYTLSEAAALVKDITTTKFDASVDI
                     DVRLGIDPRKSNQMVRGVVSLPNGTGKVVRVLALVTPDKEAEAREAGADHVGLDEWIE
                     KIKAGWTDVDVIITMPSVMGKIGQLGRILGPRGLMPNPKSGTVTMEVGKAVKEVKQGK
                     IDFKVDKAGIVHASIGKVSFEPHKIVENAREFLFMINKLKPASAKGTYVRSIFLSSTM
                     SPGLKVDPRSLDV"
     gene            complement(924..1364)
                     /gene="rplK"
                     /locus_tag="PLA17_09075"
     CDS             complement(924..1364)
                     /gene="rplK"
                     /locus_tag="PLA17_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPK85608.1"
                     /translation="MAKEVAGLIKLQIKGGAANPSPPVGPALGSKGVNIMEFCKQFNA
                     RTQDKAGKVIPVIITVYTDKSFDFVTKTPPIAVQLIEASKAKKGSPEPNRARVASVTW
                     NQVKAIAEDKMQDLNCFTLESAMKMVAGTARSMGIAVTGAFPGN"
     gene            complement(1453..2001)
                     /gene="nusG"
                     /locus_tag="PLA17_09080"
     CDS             complement(1453..2001)
                     /gene="nusG"
                     /locus_tag="PLA17_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPK85609.1"
                     /translation="MSENVKKWYVLRAIGGKEKKVKEYLDNEIARRNLQDYISQVLIP
                     MEKVYQIRSGKKVSKERTFFPGYVIVEAALVGEVPHILRNVPNVLGFLGSQSGEPIPM
                     RKAEVNRILGKVDELAASEEELNVPFVVGETVKVIDGPFNSFTGVIEEVNEEKRKLKV
                     MVKIFGRKTPLELSFMQVEKEN"
     gene            complement(2020..2211)
                     /gene="secE"
                     /locus_tag="PLA17_09085"
     CDS             complement(2020..2211)
                     /gene="secE"
                     /locus_tag="PLA17_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPK85610.1"
                     /translation="MKIGTYFKESYNELVHKVTWPSWNELQSSATLVLITSVILAIVI
                     WAMDFFFDKVMGFVYGLLY"
     gene            complement(2230..2302)
                     /locus_tag="PLA17_09090"
     tRNA            complement(2230..2302)
                     /locus_tag="PLA17_09090"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2267..2269),aa:Trp,seq:cca)
     gene            complement(2370..3557)
                     /gene="tuf"
                     /locus_tag="PLA17_09095"
     CDS             complement(2370..3557)
                     /gene="tuf"
                     /locus_tag="PLA17_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPK85611.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITMVLAKKGLSEI
                     RDFDSIDNAPEEKERGITINTSHVEYSTANRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPRIVVFLNKVDLVDDEELIDLVEMEVRDLLSF
                     YKFDGDNAPVIRGSALGAMNQEPKWEEKVMELMDAVDEYIPIPPRDNEKPFLMPVEDI
                     FSITGRGTVATGRIETGIVHTGDELEIVGLGSEKRKTVCTGVEMFRKILDQGEAGDNV
                     GLLLRGVDKKEIKRGHVLAKPGSITPHTRFKAEVYVLKKEEGGRHTPFHNSYRPQFYL
                     RTLDITGEIKLPEGVEMVMPGDNVTITVELIYPVAINKGLRFAIREGGRTVGAGAVVE
                     ILN"
     gene            complement(3600..3671)
                     /locus_tag="PLA17_09100"
     tRNA            complement(3600..3671)
                     /locus_tag="PLA17_09100"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3637..3639),aa:Thr,seq:ggt)
     gene            complement(3678..3750)
                     /locus_tag="PLA17_09105"
     tRNA            complement(3678..3750)
                     /locus_tag="PLA17_09105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3715..3717),aa:Gly,seq:tcc)
     gene            complement(3812..3894)
                     /locus_tag="PLA17_09110"
     tRNA            complement(3812..3894)
                     /locus_tag="PLA17_09110"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3858..3860),aa:Tyr,seq:gta)
     gene            complement(3943..4016)
                     /locus_tag="PLA17_09115"
     tRNA            complement(3943..4016)
                     /locus_tag="PLA17_09115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3980..3982),aa:Thr,seq:tgt)
     gene            complement(4129..4428)
                     /gene="raiA"
                     /locus_tag="PLA17_09120"
     CDS             complement(4129..4428)
                     /gene="raiA"
                     /locus_tag="PLA17_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPK85612.1"
                     /translation="MNIQIHSIRFDADKKLIDFVTQKVKKLSQVSNDIVSAEVFLRLD
                     KDDERENKITEIKIEYPRGPLFARKQSKTFEEATDLVISALKKQIVKQKEKMRGE"
     gene            complement(4454..>5343)
                     /locus_tag="PLA17_09125"
     CDS             complement(4454..>5343)
                     /locus_tag="PLA17_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPK85613.1"
                     /translation="FFMNIKESFLQYIGTEKRYSRHTVTSYKNDIDQFTGWLDEVCPG
                     VDLAGVTHADVRGWMVSLLEGGFTAGTVHRKMSALRTLFRYMRRRELITADPMAGLKL
                     PKKPKQLPVFVAEDALMKLLDEFRFGDNFSGIRDRTVVEFLYLTGMRRSELTGLRDAD
                     VDLSAGQVRVTGKRNKQRVIPLTAGFVKSLRLYIEARDGQGFQGGWFFVTDRGNKMYD
                     KAVYNIVTRYLAMVTTIEKKSPHVLRHTFATHLLNYGADLNSIKELLGHASLSATQVY
                     THNTFEQLKKIYNQAHPRA"
BASE COUNT         1302 a         1388 c         1175 g         1478 t
ORIGIN      
        1 cagggccctt ttcaggagag tgttcttcac gacaaccagc ttgatctggt tctcaaaaca
       61 cttccttctg aggtcactgg tatcagcagc attcagctct gcagtgtcag tcaggtaaaa
      121 gtggctgtac tcctgcaggg tagcagccag actgttaatt atatcggctt tttcttctct
      181 tctcataatc tctgcattag tgcttacggt taaacatcaa ggctcctggg atcaaccttc
      241 aggcccgggc tcattgtact cgagaggaag atacttctga cgtatgttcc tttcgctgac
      301 gcgggtttga gcttgttgat cataaagagg aattcccttg cgttttcaac gatcttatga
      361 ggttcgaaag atactttccc gatagaagca tgtactatac cggctttatc tactttaaag
      421 tcgatttttc cctgcttaac ctcctttaca gccttaccga cctccatggt taccgtaccg
      481 cttttggggt tcggcatgag accacgggga ccgagtatac gtcccagctg acctatctta
      541 cccattactg agggcattgt aataatgaca tcaacatctg tccagcctgc cttgatcttt
      601 tcaatccact catcaagccc cacgtgatct gcgcctgctt ctcttgcttc agcctccttg
      661 tcgggggtta ccagggcgag aaccctgacc accttacccg tgccgttagg aagggagaca
      721 acgccacgaa ccatctgatt cgatttgcgg gggtcaatcc caagcctgac gtcaatatca
      781 acagaggcat caaactttgt agtagtgata tcttttacca atgctgccgc ttctgacaaa
      841 gtatagagtt tgccatgctc aattttcgca agggcaaact tctgattcct ggtaagctta
      901 gccatttcta ttcacttaat ttattaatta ccggggaatg cgcctgttac ggcgataccc
      961 atacttctgg ctgtcccagc caccatcttc atcgcggatt cgagggtaaa gcagttcaga
     1021 tcctgcattt tatcctcagc aatagccttt acctgattcc atgtcactga tgccaccctg
     1081 gcacggttgg gctctggtga gcctttcttt gcctttgagg cttctatcag ctgaacggca
     1141 atgggagggg tctttgtgac aaaatcaaat gacttgtcgg tatacacggt aatgattacc
     1201 gggatgacct tccccgcctt gtcctgtgtc cgggcattga actgtttgca aaactccatg
     1261 atattcacac ctttggagcc cagtgcgggc ccaacgggtg gagatggatt ggctgctccg
     1321 cctttaatct gtaatttgat taatccagca acttcttttg ccataaatta aaaatttgca
     1381 ttgtttatag cgagagcatg acagccgctt aaggaagcat tataattcac ggccgtcaat
     1441 ttccttaatt cctcaatttt ctttctctac ctgcatgaag ctgagctcga gcggggtctt
     1501 gcgtccgaag atcttgacca ttaccttgag cttgcgcttc tcttcattga cctcttcaat
     1561 tacgccggtg aagctgttga aggggccgtc tattactttt accgtctcgc ccaccacgaa
     1621 cggaacattg agttcctcct cactggctgc cagttcatct acttttccaa gaatgcggtt
     1681 gacctcagcc ttccgcattg gtatgggctc tcctgactgt gagccgagga atccgagcac
     1741 gttggggaca ttcctgagga tgtgcggtac ctcacctacg agtgcagcct ccacaatgac
     1801 atatcccggg aagaatgtac gctccttgct cacctttttg cccgaacgga tctggtaaac
     1861 cttctccatc ggtatcagca cctgcgagat ataatcctgc aggttacggc gtgcaatttc
     1921 attgtcaagg tactccttga cctttttctc cttgccgcct atagcccgga gcacatacca
     1981 cttcttaacg ttttcactca tctgtgaccg ttatgaattt taatataaca gaccatatac
     2041 aaaacccatg accttgtcaa aaaagaagtc cattgcccag atgacaatag ccaggatgac
     2101 tgaggtgatg agtacaaggg ttgcgctact ctggagttca ttccatgaag gccatgtcac
     2161 cttgtgcacg agttcattat acgattcttt aaagtatgtt cctatcttca taacaagagt
     2221 tccgtttagc acgggaggag agactcgaac tcccgacacc tggttttgga gaccagtgct
     2281 ctaccaactg agctacaccc gtgtacattt cagatggtaa ccgtacacct gtgcgcacgg
     2341 ttaccatttt acttatatag tataaggtct tagttgagga tctcaactac tgcgcctgca
     2401 ccaacagtac ggccgccttc gcgaattgcg aaacggagac ccttgttgat tgcaaccggg
     2461 taaatgagct caacagtgat tgtcacgtta tcaccgggca taaccatctc aacgccttcc
     2521 gggagcttga tttcgccggt aatgtcaagg gtacggagat agaactgcgg acggtagctg
     2581 ttgtggaacg gggtatgacg accgccttcc tctttcttga gaacgtagac ctctgccttg
     2641 aacctggtat ggggtgttat tgaacccggt tttgcaagca catgacctct cttgatctct
     2701 ttcttgtcaa cgccgcggag gagcagacca acgttgtcac ctgcttcacc ctggtcaaga
     2761 atcttgcgga acatttcaac tccggtgcaa acggtcttgc gcttctctga accgaggccg
     2821 acaatctcaa gctcgtcgcc ggtgtgaacg ataccggttt cgatacggcc ggtagcaacc
     2881 gtaccacggc ctgtgattga gaagatatct tcaacaggca tcaggaaagg cttctcgttg
     2941 tcacggggag gaatggggat gtattcgtca actgcatcca tcagttccat aaccttctct
     3001 tcccacttcg gttcctggtt cattgcaccg agtgctgaac cgcggatgac gggagcattg
     3061 tcaccatcaa acttgtagaa tgagagcaga tcacgcactt ccatttcaac gaggtcaata
     3121 agttcctcat catcaacaag gtcaaccttg ttgaggaaga caacaatcct cggtacattc
     3181 acctggcgtg ccagaaggat atgttcacgt gtctggggca tcgggccgtc agtggcggca
     3241 acaacgatga tggcaccgtc catctgggca gcaccggtaa ccatgttctt cacatagtca
     3301 gcgtgacccg gacagtcaac gtgtgcatag tgacggtttg ccgttgaata ttcaacgtgc
     3361 gaagtgttga tggtaatacc cctttccttt tcctccggag cattgtcaat tgaatcaaag
     3421 tcccttattt ccgagagacc cttctttgcg agaaccatcg taatagcagc cgtcagggtg
     3481 gtcttgccgt ggtcgacgtg accgatcgtt ccgatgttta cgtgtggctt cgacctgtca
     3541 aatttttctt ttgccataat aaaagcttat taatttaaac ttacatccaa aatcgtttga
     3601 gccgatgatg agagttgaac tcatgacccc ttccttacca aggaagtgct ctacccctga
     3661 gctacatcgg catgctgagc gggagacgga attcgaatcc gcggccctta gcttggaagg
     3721 ctaatgctct gccaactgag ctactcccgc ttattatagt taaccggcac accgcttttt
     3781 ttctgaaaaa aagaacgttg cactgtagta gtggggagag caggattcga acctacgaag
     3841 tcatcagaca acagatttac agtctgcccc agttggccac tttggtatct ccccaacact
     3901 acagcgcatg cctggttaga catacatgcc tctcatacaa tgagccgatg aagggattcg
     3961 aacccttgac ctgcagatta caaatcagct gctctggcca actgagctac atcggcaatt
     4021 gaaagaacga ccgatttctg agaaattacc ctcaaaaatc ggtttgcaaa tgtagaaatt
     4081 ataaattcaa aactcaaatt tatccgaagt ttttttgaaa cgaaaagatt actccccccg
     4141 catcttctcc ttctgtttga ctatctgctt cttaagagca ctaatcacaa ggtcggtcgc
     4201 ctcctcgaaa gttttactct gcttgcgggc aaaaagcggc cccctgggat actctatctt
     4261 gatctccgta atcttgttct ccctctcatc atctttatcc agcctcagaa acacttcggc
     4321 gctgacaatg tcgttactta cctgagaaag cttttttacc ttctgagtaa caaagtctat
     4381 cagcttcttg tctgcatcaa accttatcga atgaatctga atgttcatgg catacctcct
     4441 gttaagttaa tgattatgct cttggatgag cctgattata gattttctta agttgttcaa
     4501 atgtgttgtg cgtgtatacc tgggtggccg agagactggc atggcccaga agctccttta
     4561 tcgaattgag atcagcgcca taattaagca ggtgcgtggc aaaggtatgc ctgagaacat
     4621 gagggctctt cttttcaatg gtggtaacca tggcaaggta tctggtgact atgttgtaaa
     4681 cagccttgtc atacatttta ttacccctgt cagtgacaaa gaaccatcct ccctggaaac
     4741 cctgcccatc acgcgcttca atgtataaac gaagtgattt tacaaatcct gcagtaagag
     4801 gaataaccct ctgcttgttc ctcttgcccg taaccctgac ctgccccgca gagagatcaa
     4861 catccgcgtc acgcagacct gtcagctccg acctcctcat gcctgtaaga tacaaaaact
     4921 ccacaacagt tctgtcacgg attcccgaaa aattatctcc gaacctgaat tcgtcaagca
     4981 gcttcatgag agcatcctcg gcaacaaata cgggaagctg tttcggcttt ttgggaagct
     5041 tcaaccctgc catcggatcc gcggtaatga gctcacgcct gcgcatatac cggaagagtg
     5101 tcctgagtgc tgacatcttc ctgtgaaccg taccggccgt aaagcccccc tcaagaagcg
     5161 ataccatcca gccgcgtaca tcagcatgag ttacgcctgc aagatcaaca cccgggcata
     5221 cttcatcgag ccagcccgtg aactgatcta tatcattttt ataggatgtt acagtgtgcc
     5281 gtgaataacg cttctctgta ccgatgtatt gaaggaacga ttcctttata ttcatgaaga
     5341 aag
//
LOCUS       DAOPBK010000162         5318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13201_length_5318_cov_4.612198, whole genome shotgun sequence.
ACCESSION   DAOPBK010000162 DAOPBK010000000
VERSION     DAOPBK010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13201_length_5318_cov_4.612198"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="buk"
                     /locus_tag="PLA17_09130"
     CDS             complement(<1..890)
                     /gene="buk"
                     /locus_tag="PLA17_09130"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPK85614.1"
                     /translation="MMKKLILAINPGSTSTKFALYDGKEKLMEKTLRHSAEELEGFVT
                     MTDQLPFRYRVIIDALRKEQADLKEITAVVGRGGLVNPIESGMYEVNDLMKRHLSEAV
                     NGEHASNLGALLADMIVKELPGAKAYIVDPVVVDELEPIARLSGHPLISRRSIFHPLN
                     QKAVARIYASSIGRKYEDLNLIVAHMGGGISVGAHRHGRVVDVNNALNGDGPFSPERS
                     GGLPTLQLSELCFSGKYKLPEVNSMLAGKGGIVAYLGTNSFKEASERASGGDEYAALI
                     VEGCAYQIAKEIGAMSAVLAG"
     gene            complement(893..1807)
                     /locus_tag="PLA17_09135"
     CDS             complement(893..1807)
                     /locus_tag="PLA17_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPK85615.1"
                     /translation="MITNLRQMADTVRSSGIKKRIAVAWAQDPNTIGSLHRAVAEGFA
                     EAIMIGRRDRILAVCQETGADPRLFTLVESPDEKSAAELAVSFTRNGEADVVMKGLVG
                     TDKFLRAVLDREKGLLPPGAVMSYVCAVEIPAYHKLLFVTDTAVIPFPDLGQKVAMAK
                     YAIEMAHSFGIETPKVALIGASEKVNERFPNTLDYAEMCRMVQSGEITGCIMDGPLDL
                     FLACDPESVRIKGVDTPVDGDADILLFPSLEACNPFYKGLMLFGGGELAGMICGTSKP
                     VVLMSRSESELSKYYCVALACLMASRKK"
     gene            complement(1818..2717)
                     /locus_tag="PLA17_09140"
     CDS             complement(1818..2717)
                     /locus_tag="PLA17_09140"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPK85616.1"
                     /translation="MALKHLSEFKQLLAGKPARRLVLAAAQDHNSLSSVLRAANDGFI
                     DPILVGKKEEIYNLAAQNNFDLTGVRVVHEPDSEMAIEIAVRMVRSNQADILMKGKVG
                     TSNLLKGVLNKEWGLRSGALLSHIAIFEVEAYHKLIAVTDVAMNIAPNLKDKISIVNN
                     SVACLNKLGIKMPKVAVLGAVEMVTENMQATLDAALLSKMNQRDQIRNCIIDGPLAFD
                     NAISLESANHKGIRSDVAGDTDLLLMPDIEVGNVLYKSLVFFANAGVAAVILGATAPI
                     VLTSRSDSDHAKYYSIVLAAAIS"
     gene            complement(2730..3941)
                     /locus_tag="PLA17_09145"
     CDS             complement(2730..3941)
                     /locus_tag="PLA17_09145"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPK85617.1"
                     /translation="MNILVLNCGSSSIKYQLFDMGDDTRVLAKGLIERIGITDSILTH
                     RPAGKDPYKVVLDIPDHTVGINMVMEALVHPVHGVIKTISEIKAVGHRVAHGGESFKE
                     SVLIDNDVKRGIEKCADLAPLHNPAHLKGILSCEKLLPGVPQVAVFDTSFHQTMADYV
                     YMYAIPYEYYEKYRIRKYGFHGTSHKFVAEKACRILNMDMYNSKIITCHLGNGASITA
                     IENGKSTDTSMGFTPVDGLIMGTRTGEIDPGALLFLADKEELSVSGINNLINKKSGVA
                     GISELSSDMRDLGEAADEGNPKAILALNMYSYKIKKFIGAYVAAMNGLDLLVFTAGIG
                     ENDFSTRQMICTGMEYMGIELDEDVNNKVKGKDMLISKPSSKVKVMVITTDEELVIAS
                     DTQTIVEKLVY"
     gene            complement(3955..4962)
                     /gene="pta"
                     /locus_tag="PLA17_09150"
     CDS             complement(3955..4962)
                     /gene="pta"
                     /locus_tag="PLA17_09150"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPK85618.1"
                     /translation="MELLEQIKSNARRHNKMIVLPEGTEERTLKAADAILAEGIARIT
                     LLGKPAEIEAAAASFGLKNIGKAVIINPVDYPGKKHYIDTLVELRKNKGLTVEEASRL
                     IEDPLYFAVMMIKCGDADGEVAGAQNVTGDVLRPAFQIVKTAPGISVVSGAFIMILKD
                     KEFGEDGIIVFADCAVHPDPTDKELAEIAVATARTTKTIVGVEPRVALLSFSTMGSAK
                     HAMVDKVVSATKMAKEMAPELMFEGELQADAALIEAIGQKKAPGSKIAGKANVLVFPD
                     LECGNIAYKLVERLAHARAIGPVLQGMAAPINDLSRGCSVDDIINVIAITANQAAGLN
                     R"
BASE COUNT         1232 a         1425 c         1194 g         1467 t
ORIGIN      
        1 ccggccagaa cagctgacat tgcacctatc tccttggcta tctgatatgc gcatccctct
       61 acaatgagag cagcatattc atcgccaccg gaagcccgct ctgatgcctc cttgaagctg
      121 tttgtgccaa ggtatgcaac tatcccaccc tttccggcca gcatggaatt tacctctggc
      181 agcttgtact ttccgctgaa acagagctcg gagagctgca atgtgggcaa tccgccactt
      241 cgttcaggtg agaaaggtcc gtcgccgtta agtgcattat tgacatcgac aacccttccg
      301 tgacggtgtg ctccaacact tatgccacca cccatatgcg ctacaatcag gttaaggtct
      361 tcatacttcc ttccgattga tgaggcatat atccttgcaa cagctttctg gttcagcgga
      421 tgaaatatcg atctcctcga tatcagcggg tgtcctgaaa gcctggctat cggctcaagc
      481 tcatcaacca ccacaggatc aacaatatat gcttttgctc caggaagctc tttcacaatc
      541 atatcagcca gcagcgcacc gaggttgctg gcatgctctc cgttgaccgc ttctgaaaga
      601 tgtctcttca tcaggtcatt gacctcgtac atgcctgatt cgataggatt caccagtcct
      661 cccctgccaa ctacggctgt aatttccttc agatcagcct gttcctttct gagtgcatct
      721 ataattaccc ggtacctgaa aggcagctgg tcagtcatgg taacaaagcc ttcaagctct
      781 tcagcactgt gtctcagagt tttctccatg agtttttctt tcccgtcgta gagagcaaac
      841 ttggtacttg ttgaaccggg atttatagca aggataagct tcttcatcat tatcattttt
      901 ttcgtgaagc cataaggcat gcaagggcga cgcagtagta ctttgacagc tcactttcgc
      961 ttcttgacat cagcaccacg ggtttggagg tgccgcagat cattccggca agttctcctc
     1021 cgccgaacag cataagtccc ttgtagaacg gattgcatgc ttccagcgat gggaaaagca
     1081 gtatatcggc atctccgtct accggtgtat caactccctt tatcctgacg ctttccgggt
     1141 cgcaggccag gaagaggtcg agcgggccat ccatgatgca gcccgttatc tctccgctct
     1201 gaaccatcct gcacatttca gcataatcaa gcgtgtttgg gaagcgttca tttacctttt
     1261 ccgatgcccc tatcagtgcc acctttggag tttcaatacc aaaactgtgt gccatctcaa
     1321 tggcatactt cgccatggct accttctgac caaggtcagg gaatggaatg actgccgtgt
     1381 cggtaacaaa cagcagcttg tgatatgctg ggatctccac ggcacagaca taactcatca
     1441 ctgctcccgg gggcaacagc cccttctccc tgtcaagcac tgccctgagg aacttgtcag
     1501 tgcccacaag gcctttcata acaacatcag cctcaccgtt cctggtaaat gacactgcca
     1561 gctccgcagc gctcttttca tcaggagatt caaccagtgt aaataatcgg gggtcagctc
     1621 ctgtctcctg gcagacagca agaatccggt cacgccggcc aatcattatg gcctctgcaa
     1681 atccttcagc aacagccctg tgaagtgacc ctatggtatt cggatcctga gcccaggcaa
     1741 cggctattct ctttttaatt cctgacgacc ttacggtgtc agccatctgc ctgagatttg
     1801 taatcattct attcctttca tgatattgct gcggcaagca ctatactgta atacttcgca
     1861 tgatcagagt cgcttctcga agtaagcact attggtgccg ttgctcccag aatcactgct
     1921 gcaacgccgg cgttggcaaa gaagaccagt gatttataga gaacatttcc aacctcaata
     1981 tcaggcatca gcagcaagtc ggtgtcgcca gccacgtcac ttctgatacc cttgtggttg
     2041 gcgctctcaa ggctgattgc attatcaaag gccagtggcc cgtcaatgat acagttcctt
     2101 atctggtccc tctggttcat ttttgagagc agtgctgcat caagggtggc ctgcatgttc
     2161 tcagtgacca tctcaacagc tccaagcaca gccactttcg gcatctttat tcccagcttg
     2221 ttgaggcagg caacggaatt gttgactatg gatatcttgt ccttgagatt gggagcaata
     2281 ttcattgcaa catcagttac cgcgatgagc ttgtgatagg cttcaacctc aaagattgca
     2341 atgtgtgaga ggagtgcacc tgacctgagc ccccactcct tgttcaggac tcccttgagc
     2401 aggtttgagg tgcctacctt tcctttcatg aggatgtctg cctggttgct gcggaccatc
     2461 ctgacagcta tctcgatagc catttccgaa tcgggctcgt gcacgaccct tacaccagtc
     2521 aggtcaaaat tgttctgagc tgcaagatta tatatctctt cttttttgcc gacgagtatg
     2581 ggatcaatga aaccatcatt tgcagccctg agaaccgagg agagtgaatt gtggtcctgg
     2641 gcggcagcca gtaccaggcg ccttgcaggc ttgcctgcaa gcagttgctt gaattcagag
     2701 aggtgcttta aagccatttc agagagtgtt taataaacca gtttctcaac tatagtctgg
     2761 gtatcggatg caatcaccag ttcctcgtct gtggtaatca ccatcacctt cactttcgag
     2821 gaaggctttg agatcaacat gtcttttccc ttcaccttgt tgttcacatc ttcgtcaagc
     2881 tcgatgccca tatactccat cccggtgcag atcatctgcc ttgtactgaa gtcgttctcg
     2941 cctattccgg cagtaaagac gagcaggtcg agtccattca ttgccgcaac atatgcaccg
     3001 atgaacttct tgatcttgta tgaatacata ttaagcgcca gtatcgcttt cgggttacct
     3061 tcgtcagcag cctctccaag gtcgcgcatg tcggaggaga gctctgaaat gccggcaacg
     3121 ccgctcttct tgttgatcag attattgata ccactgacgc taagctcttc cttgtcagcc
     3181 agaaaaagca atgcgcccgg atcaatctca cctgtacggg tacccatgat cagtccgtca
     3241 accggggtga aacccatcga tgtatctgtt gacttaccat tttctatagc agttattgag
     3301 gctccgttgc caaggtggca ggtgattatt tttgaattgt acatatccat attcagtatc
     3361 ctgcaggcct tttccgcaac gaacttgtga ctggtgccat ggaatccgta tttgcggatg
     3421 cggtatttct cataatactc ataaggaatt gcgtacatgt atacatagtc agccattgtc
     3481 tggtggaacg aggtgtcaaa gaccgccacc tgcggaactc ccggcagcag cttctcgcag
     3541 gagagtatcc ccttgaggtg tgcgggattg tgcaggggag ccagatcagc gcatttttct
     3601 attccgcgct tgacatcgtt gtctatcagt accgactcct tgaagctttc gcctccgtgc
     3661 gctacacggt gacctacagc ctttatctcc gaaattgttt tgatgacgcc atgcacagga
     3721 tgtaccagtg cctccataac catgttgata ccgactgtat ggtctggtat gtcaaggacc
     3781 accttgtagg gatccttgcc ggcgggcctg tgagtaagga tgctgtccgt aatgccgatt
     3841 ctttcaatca gtccttttgc cagtaccctg gtgtcgtcac ccatatcgaa aagctggtac
     3901 ttgattgaag aactgccgca gttgagtacg agaatattca tgattttctt tgtgttatct
     3961 gtttaaacct gctgcctggt tggcagttat ggcaattacg ttgatgatgt catctactga
     4021 acaacccctc gagaggtcat tgataggtgc tgccatgccc tgaagcacgg gaccgatggc
     4081 gcgtgcatgt gccagtctct caaccagctt gtaggctatg tttccgcact ccaggtcggg
     4141 aaaaacgaga acgttggcct tgcctgctat tttgcttccc ggagctttct tctggccgat
     4201 tgcctctatc agggcagcat ctgcctggag ctcaccctcg aacatcagtt ccggagccat
     4261 ctcctttgcc atttttgtgg cactgaccac cttgtcaacc atcgcgtgct ttgcgctgcc
     4321 catggtggag aagctgagca gggcaaccct gggttccaca ccgacaattg ttttggtggt
     4381 acgtgccgtt gcaactgcaa tctccgccag ttccttgtca gtgggatcag ggtgaactgc
     4441 acagtcggcg aagacaatta taccgtcctc accaaactcc ttgtccttca atatcatgat
     4501 gaatgcaccc gaaaccactg atatgcccgg ggctgtcttt acaatctgaa atgcaggcct
     4561 cagcacatcg cctgtaacgt tctgggctcc cgcgacttct ccgtcagcat caccgcactt
     4621 gatcatcatc actgcgaaat aaagcggatc ttcaatcagc ctggaagcct cttcaactgt
     4681 aagtccctta ttcttacgca gttccaccag cgtgtcaatg taatgcttct tcccgggata
     4741 atcaacagga ttgattatta ccgcttttcc gatattcttc agaccaaacg aggctgcagc
     4801 agcctctatt tccgcaggtt ttccgagaag ggtaattctc gctatcccct cagccagaat
     4861 tgcatctgcc gctttaaggg tcctctcttc agttccttca ggtaatacaa tcattttgtt
     4921 atgccttctg gcattcgatt tgatctgttc aagcaattcc attggatatc agtaatttag
     4981 ttaaagtatt gtgtcgggat aaaagtacta aacaaagagt aatcccgcaa tgatcaacaa
     5041 tatgattata atcagtattt taaaatttaa gtcatttttt ttcgaaaaca actttgcggc
     5101 cctgccgcga acccttcaaa ttcctatttt tgatgctgat tttactcaga tggatgatca
     5161 acgcacagcc acattgacag aactgctgaa ctcaggcatt gagggcgagg tacgcactga
     5221 cagaagttcc aggctcatgt atgccaccga tgcctccatc tacagggagg agccgctggc
     5281 gatagtcatt ccgcgcaatg ctgatgacat aaaaaagc
//
LOCUS       DAOPBK010000163         5289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13275_length_5289_cov_5.132786, whole genome shotgun sequence.
ACCESSION   DAOPBK010000163 DAOPBK010000000
VERSION     DAOPBK010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13275_length_5289_cov_5.132786"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /locus_tag="PLA17_09155"
     CDS             <1..2088
                     /locus_tag="PLA17_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HPK85619.1"
                     /translation="DAESVPDKKEEAANEAVPEEEAEAVTEDVSDKEEEAANEVVPEE
                     EVDTDAESVPDKKEEAANEAVPGKEADAAAGTEAREAEEIVAGEVKEEKEAVPDESSS
                     EDDTEETESVNYDDIELPPVDYSGFSRKELVETLALIVENRPPSEIMDDVARIKEFFY
                     KKTKAEFNEKRLNFAKEGGNIEEFRPEPDELESTIKTVLETYRNRRSDFNRILESEKQ
                     DNLRKKLEIIEQIKDLVNREEAINKTFQEFRSLQNEWHSIGIVPQAAVKDMWENYHHS
                     VEIFYDYIKINRELRDLDLKKNLEAKIVLCEKAEELLLEPNAVNAFKQLQDLHNHWRE
                     IGPVPHENKNEIWERFREATSKVNKRHHEYFEKQKEEQKRNLEAKTALCEKVEEIAAG
                     EILTFADFREKSDTILECQKLWKTIGFAPKKHNNKIYQRFRAACDTFFEKKRAFFAEN
                     KELQARNLQLKTELCLKAEALQESDDWKDTSDMLIRLQKEWKDIGPVPKKYSEKIWRR
                     FRKACDTFFTRKSEYFTGRETSYEDNLKAKLAILDELEAFDPGEDMRAGFDKLKDIQK
                     RWTEIGYVPFNRKEEIARRYKEALNRQFDKLKLDEEDKNILRYSSKVDSAKSNPRAAR
                     KMRGEREKFYSKIKQLESDIVLWENNIGFFAKSPNADNMIREVEEKIAEARRNIKILE
                     EKVKLIDNSMYEE"
     gene            2126..3730
                     /locus_tag="PLA17_09160"
     CDS             2126..3730
                     /locus_tag="PLA17_09160"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPK85620.1"
                     /translation="MAEVKYVFVTGGVTSSLGKGIISASLAKLLQARGYSVTIQKLDP
                     YINIDPGTLNPYEHGECYVTVDGAETDLDLGHYERFLNVHTTQVNNVTTGRIYQSVIN
                     KERRGDYLGKTVQVIPHITDEIKRRIKIVGSGGNYEIVITEIGGTVGDIESLPYIEAV
                     RQMRWEMGPRNCVVIHLTLVPFLQASGELKTKPTQHSVKMLLEDGIQPDVLVLRTEHP
                     LNEDLRKKVALFCNVEPGAVVESIDVTTIYEVPLMMQKEKLDLTVLRKLGLPQGEEPS
                     LSEWKEFLHKLKNPAHQVKIALVGKYVELADAYKSISESFIHAGAANETAVEMAMIHS
                     EEITDANYGTMLDGYDGIVVAPGFGERGIEGKILAANYARKNDIPFLGICFGMQCAVI
                     EFARNVLGLEGAHSAEINPKTKHPVIDLMEEQKSIAVKGGTMRLGSYKCELLHGTKAW
                     ECYRKDKISERHRHRYEFNNKYITDFRNAGMIASGTNPESGLVEIIEIPSHRWFVGVQ
                     FHAEYSSTVIKPHPLFIGFVRACISK"
     gene            3759..>5289
                     /gene="yidC"
                     /locus_tag="PLA17_09165"
     CDS             3759..>5289
                     /gene="yidC"
                     /locus_tag="PLA17_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPK85621.1"
                     /translation="MDRNTVTGIILIFLIFLGFSLYNNHRMGKFFEAEKEYADSLYAA
                     GNYDLAREAYIRALNYKPKDQPTIQKVTELNKQLGLGSVQQPDSAALVAAGTTDTTAA
                     LPGSMPVRVTDSTALGVFSSRTRGEDELYTLENDLMVMKIAARGGKPYSVMLKEYKTH
                     DGRPVVLFDGDSTVFGYKFFTVDNKPIRTDELYFTPVDYHGPVVASGSPVSLTMRLEA
                     SDGAYIDYVYTMQPDDYMIDFESVFNSLSSVIPANTTSMALDWKMYIPQQEKGRQNED
                     MYTGLKYKVYQEDVVDLGGTRMQRQKKDVVTESLMKTDWIAYKDQFFSSVLVSDDFFL
                     NAVVTSTNADPTSRYLRFFESEIGVPFAHSDRSRVKMQFYFGPNSFTLMKKYDIELEK
                     LVFLGRNIIRWINQFVIIPIFNLLANYIESYGLIILILTLIIKLALSPLTHRSFISQA
                     KMRVMKPMVDEIAKKYPKQEDAMKKQQATMDLYKKAGVSPMGGCLPMLLQMPILFAMF
                     RF"
BASE COUNT         1582 a         1151 c         1393 g         1163 t
ORIGIN      
        1 gacgccgaat ctgtacccga caagaaggaa gaagctgcca atgaagccgt gcctgaagag
       61 gaggcagagg ctgtcactga agacgtgtct gacaaagagg aggaagctgc caatgaagtc
      121 gtacctgaag aggaggtaga tactgacgcc gaatctgtac ccgacaagaa ggaagaagct
      181 gccaatgaag ccgtgcctgg caaggaggca gatgctgccg ccggtaccga agccagggaa
      241 gctgaagaaa tcgttgccgg ggaggtaaaa gaggaaaaag aagctgttcc tgatgaatca
      301 tcatctgaag atgacacaga ggaaacggaa tcagtcaact atgatgatat tgagctgccc
      361 ccggtagact acagcggttt ctcaaggaag gaactggtgg agacgcttgc cctcatagtt
      421 gagaaccgtc ctccctccga gatcatggat gatgttgccc ggattaagga gttcttttac
      481 aagaagacaa aggctgaatt caatgagaag aggctgaatt ttgcaaagga aggtggaaac
      541 attgaagaat tcaggcccga acccgatgaa ctggagagca cgatcaagac agttctggag
      601 acctaccgca accgaagaag tgatttcaac cggatactgg agagtgagaa acaggacaat
      661 ctgcgcaaaa agcttgaaat aatagaacag ataaaggatc tcgtgaaccg tgaggaggct
      721 atcaacaaga ccttccagga gttccggagt cttcagaatg aatggcatag catcggcatt
      781 gtgcctcagg ctgccgtcaa ggacatgtgg gagaactatc atcacagtgt cgagatcttc
      841 tatgactata taaagattaa ccgtgaactg agagaccttg acctgaagaa gaaccttgag
      901 gccaagatag tcttgtgtga aaaggctgag gaactgcttc ttgaacccaa tgctgtcaac
      961 gccttcaaac aattgcagga cttgcacaat cactggcgtg agataggtcc ggttcctcat
     1021 gaaaacaaga atgagatctg ggagcgtttc cgcgaagcaa cctcaaaggt aaacaagagg
     1081 catcatgagt acttcgagaa acagaaggaa gaacagaaac gcaaccttga agccaaaacc
     1141 gcactctgtg aaaaggttga ggagatagca gcaggagaga ttctcacctt tgcagatttc
     1201 agggagaagt cagatactat ccttgaatgt cagaaactgt ggaagacaat cggctttgct
     1261 cccaagaagc acaacaacaa gatttaccag cggttcaggg cagcctgcga caccttcttt
     1321 gaaaagaagc gggcattctt tgccgagaac aaggaacttc aggcaaggaa cctgcagctc
     1381 aagactgagc tctgtctcaa ggctgaagca ttgcaggaga gcgatgactg gaaggatacg
     1441 agtgatatgc ttatcaggct gcagaaagaa tggaaggata tcggacccgt cccgaagaaa
     1501 tactccgaga agatttggag gcgcttccgc aaagcctgtg acaccttctt tacaagaaaa
     1561 tccgaatatt ttacaggcag ggagacctcc tatgaagata acctcaaggc caagctggca
     1621 attctggatg agcttgaggc atttgacccg ggtgaagaca tgagggcagg ttttgacaag
     1681 ctgaaggata tccagaaaag gtggacagag atcggatatg tgcccttcaa caggaaggaa
     1741 gagatagccc gcagatacaa agaggctctc aacaggcaat tcgacaaact gaagcttgat
     1801 gaagaggaca agaatatcct tcgctacagc agcaaggttg acagtgccaa atcaaatcct
     1861 cgcgcagcac gaaaaatgag aggcgaacgg gaaaaattct acagtaagat caaacagctt
     1921 gagagcgata tagttctctg ggagaataat ataggcttct ttgcaaaatc gcccaacgct
     1981 gacaacatga tcagggaggt ggaggaaaag atagccgaag ccaggaggaa tatcaagatt
     2041 cttgaggaaa aggtcaagct gattgacaat tcgatgtacg aggaatagta attctctttc
     2101 agccaataac ataagatttt cagcagtggc agaggtaaag tatgtttttg taaccggcgg
     2161 ggtcacatca tcgctgggca agggtatcat ttctgccagc cttgcgaagc tccttcaggc
     2221 acgcggctat tctgtgacca ttcagaagct tgatccttat ataaatattg atccgggaac
     2281 gcttaatcca tatgagcatg gagagtgtta tgtcaccgtt gacggcgccg agactgatct
     2341 tgatctcggt cattatgaac gtttcctgaa tgttcatacc acccaggtca acaacgtcac
     2401 cacagggagg atttaccagt ctgtaataaa caaggagcgc aggggtgatt atcttggaaa
     2461 gaccgtacag gtcatccctc acatcactga tgagatcaag cgtagaataa agatcgtggg
     2521 ttcaggtggc aactatgaga ttgtcattac ggagattgga ggaacggtag gtgatattga
     2581 atcgctaccc tatattgagg cagtgaggca gatgcggtgg gagatggggc cccggaactg
     2641 cgtggtgata cacctgacgc tggtgccatt cctgcaagct tccggtgaac ttaagaccaa
     2701 acccacccaa cactcggtaa aaatgctcct ggaagacggg atacagcctg acgtgctggt
     2761 gcttcgcacc gaacatcccc tgaatgagga tctgagaaag aaagtggccc tcttctgcaa
     2821 cgttgaaccc ggggccgtgg tggagtcaat tgatgtcacc accatatatg aggtgcctct
     2881 catgatgcag aaggagaagc ttgacctgac agttctgcgg aagcttggcc tgccacaggg
     2941 tgaggagcct tccctctcag aatggaagga gttcctgcat aagcttaaaa accctgcaca
     3001 ccaggtgaaa attgcgctgg taggcaaata tgttgagctg gctgacgcct acaaatcaat
     3061 atccgagtca tttatccatg ccggggcagc aaatgagacg gcggtggaga tggctatgat
     3121 tcattcagag gagattactg atgccaatta cggaacaatg cttgatggtt atgacggcat
     3181 tgttgtggca cccgggtttg gagaacgggg catagaaggc aagattcttg cggctaatta
     3241 tgcccgtaaa aatgatattc cttttctggg aatatgcttc ggcatgcaat gcgctgtgat
     3301 tgaatttgca agaaatgttc tggggcttga aggggctcac tctgccgaga tcaatcccaa
     3361 gacaaaacac cccgtaattg atctgatgga ggagcagaaa agcattgcgg tgaaaggcgg
     3421 aaccatgagg ctgggaagct acaaatgcga actccttcat ggaaccaaag cctgggagtg
     3481 ctacaggaag gacaagatat ccgaacgtca ccgtcacagg tacgagttca acaataaata
     3541 cataaccgat ttccgcaatg cgggcatgat tgcaagcggc accaaccctg agtcaggact
     3601 ggtcgaaata attgagatac cttctcacag gtggttcgtt ggagtgcagt ttcacgctga
     3661 gtacagcagc actgtgatta aaccccaccc tctcttcatc gggtttgtca gggcatgtat
     3721 cagcaaataa taactatata acagggaaat aaggaataat ggatagaaat actgtaaccg
     3781 gcatcattct gatcttcctg atctttctgg gattcagcct gtacaacaat cacaggatgg
     3841 gaaaattctt tgaagcagag aaggaatatg ccgattctct ttatgccgcc ggcaactatg
     3901 atctggcccg cgaggcatat ataagggcgc tgaattacaa accgaaggat cagcctacta
     3961 ttcaaaaggt aacagaactg aacaaacagc tgggtctggg aagtgtgcag cagcctgatt
     4021 cagccgctct agtggctgca ggaacaacag atacgacagc tgccctgcca ggttcaatgc
     4081 ctgtgagggt gactgacagt acggcattgg gagtcttcag ttccagaacc cggggagagg
     4141 atgagttgta tacccttgaa aatgacctca tggtcatgaa aattgccgcc aggggaggca
     4201 aaccctattc agtcatgctg aaagaataca agacgcatga cggtcgtccg gttgtgcttt
     4261 tcgacggtga ctcaaccgtg ttcggctaca aattctttac agttgacaac aaacccatac
     4321 ggactgatga gctttacttc acgccggtgg attatcatgg accggtggtt gccagcggaa
     4381 gcccggtaag cctgaccatg agactcgagg catcagacgg tgcatacatt gattatgtct
     4441 acaccatgca gcctgatgat tacatgatag actttgagtc ggttttcaac tcactctctt
     4501 cagtcattcc ggcaaatacc acttcaatgg cgcttgactg gaagatgtat atcccccagc
     4561 aggaaaaggg gaggcaaaat gaggatatgt acactggcct gaaatacaag gtctaccagg
     4621 aggatgtcgt ggaccttggc ggcaccagga tgcagagaca gaagaaggat gtggttacgg
     4681 aaagcctgat gaagacagac tggatagcat acaaggacca gttcttttca tcggtacttg
     4741 tatctgatga ttttttcctg aatgcagtgg tcacttccac caacgctgat ccaacctcca
     4801 ggtatctgag attcttcgag tcagagattg gcgtgccctt tgcccacagt gacagaagca
     4861 gggtgaagat gcagttttat ttcggtccca acagcttcac cctgatgaag aaatatgaca
     4921 tcgagcttga aaaactggtt ttcctgggca ggaacataat caggtggata aaccagtttg
     4981 tcatcatacc gatcttcaac cttcttgcca actatattga gagctacgga ttgataatat
     5041 tgatcctgac actgataatc aagctggctc tctccccgct tacgcacagg tcattcatca
     5101 gccaggccaa gatgagggta atgaaaccca tggtagatga aattgccaaa aagtatccga
     5161 aacaggagga tgcgatgaag aaacagcagg ctacgatgga tctgtacaaa aaagcagggg
     5221 taagtcccat gggcggctgt ctccccatgc ttctgcagat gcctatcctg tttgcaatgt
     5281 tccgtttct
//
LOCUS       DAOPBK010000164         5288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13278_length_5288_cov_4.425186, whole genome shotgun sequence.
ACCESSION   DAOPBK010000164 DAOPBK010000000
VERSION     DAOPBK010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13278_length_5288_cov_4.425186"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1182)
                     /locus_tag="PLA17_09170"
     CDS             complement(4..1182)
                     /locus_tag="PLA17_09170"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPK85622.1"
                     /translation="MVRILKHIAVAGFFLFVTIAFLSGQAKGTFEVVRISEISSSSDD
                     MAPVIMSDGILFCSNRKTNPFFTKKNLEGVRLYELYFAPFNEEGEPHSPVRFAPGLIR
                     DANIGPASVTTDGNTMWFTRNNSEGRRLGKKQPNRLGIYSARRSGSQWTDITPFEYNN
                     PEYNLSFPYVSADNRYLFFCSDMPGGVGSYDIYMCENVNGRWTKPENLGPVVNSSDAE
                     IHPFLHSSGRLYFASDRPGGKGGLDIWYSSLIYGSWTKPIAMDEPVNSAADDFAFYAA
                     QGGMQGYFTSNRHNFNDDIYSFESTIIRWPACDSIQKNSYCFEFIEENALKHDTIDKG
                     FRWEWDFGDGTRATGITADHCFSGPGIYDVSLDIVNLITGGIEKRLASYELEITDIEQ
                     "
     gene            complement(1187..2116)
                     /locus_tag="PLA17_09175"
     CDS             complement(1187..2116)
                     /locus_tag="PLA17_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPK85623.1"
                     /translation="MKSGLYISVLLALVLALPVDGQQVPVTSQYLTNGLVINPAYAGT
                     REVLSANLSYRKQWARINGAPQFQTLSLHSPVNKKERVSLGLMTDYLTYGVTKDVGIY
                     GFYAYSIRYGHGKLSMGLKAGFDLSNTNYNRLRFPDGNPADPLLSGDMKYSLPNMGVG
                     FYYYTEKYFVGLSVPSLLTYKRDEADQFSVTPDYSLFRTYLTAGTLVRVADMFKVKPS
                     VLIRYSMHEPLEVDLNANLIFADMLWFGCSFRIAEKAAVALLDLQVTPQLKVGYSFDY
                     QLGHLNNYTSGTHEVSLRYEFAFAVTATSPRYF"
     gene            complement(2118..>5288)
                     /locus_tag="PLA17_09180"
     CDS             complement(2118..>5288)
                     /locus_tag="PLA17_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPK85624.1"
                     /translation="WDSASVTGGVLVFMVKGVLTLNSNINVAGTGFRGGIVSQGLGWC
                     QNAHDTLRYESYNIWSVVAGYKGEGLAIRTAMGQPLYPSFMKGKGANMTGGGGGNGHF
                     SGGGGGSNYGSGSVGDQEMPSVTCGFLWTGGRPGFTVASYSTLNTGVYMGGGGGASVY
                     LTTPTTSAGGNGGGIVIIHADTIVGNGRIITANGAAANNNTALNAGAGGGGGGGSVII
                     STRHFLSAPVLSADGGRGGNNVNQNGVGGGGGGGLIWTAGTFPGTATVDGGQGGIHSS
                     GDPNRDGLPGLIRNNLNLPLNGFLFNEIYIAYNQNQLDSICEGMVPPKLTGTMPAGGS
                     GTYTYQWQRSYDNINWSNVPGTSFEYTPAASESATVWFRRVVSDGAGIIDRSLPVQII
                     VHPRITGNIIGNDTTLCYNMNPEELYPLNSGPAGGNGIYFYQWEQSADNVTWANAAGV
                     STAARYNPQALTATMHYHRRVSSGACVDVSSPVTVTILPLITNNNITADQTICEGTLF
                     SNLSGSNPVGGAGAGSYGYQWMSSTDNTNWQPAPGSATSSGYNVTNDSPGTTWYRRVV
                     YSGPLNTCQSTSNSVTLICHPAITNNVISADQTICEGTAPATIDGTVPANGAGAGTYG
                     YQWMSSSNGGSFSNISGATAEDLPGTTLTADIWYRRVVTSSACTSTSNTVHITVDPRI
                     TGFDINLPANGHDTICTGTAPGLLSGTPAGGLGTFTYAWALSTDNNYFLPLPETGQSY
                     QHGNLTATTWFRRSVTSGACSVSSVFRITVLPQITGNVITADQTVCNTHTPAALTGST
                     LSGGDGRYRYLWEGKKPQSSEWTAAPGTNNAASYQPAMLDETTQFRRHVFSGENNCCV
                     SVSAPVTVNVDIMPVNISAGADRTLLPYQLAAVLEGSFDGEGTSLWRYDVSSGNAKPV
                     FATPLEKITEVRKLEFGANTFIFAVTNGACIAPEATVTLTVPDLIIPQGVTPNNDGIN
                     DYFNVEGLEFTHNELVIINSAGAVVYRADDYRSNDPVGAWTGLDLNGREVPDGTYYYL
                     LTITGAQDLNVPEYSAHISGFIILRR"
BASE COUNT         1234 a         1392 c         1341 g         1321 t
ORIGIN      
        1 tatctattgc tcaatatcag taatttcaag ttcataggaa gcaaggcgtt tctcaatgcc
       61 cccggtaata aggttcacaa tgtcaaggct gacatcatat attcccggac cgctgaagca
      121 gtgatctgcg gttatacctg tggctcttgt cccgtcaccg aagtcccact cccacctgaa
      181 acctttatca atggtatcat gttttagtgc attctcctct atgaactcaa aacagtagct
      241 gtttttctga atcgaatcgc aggccggcca tctgatgatg gttgattcga aactgtaaat
      301 atcatcattg aaattgtggc ggtttgaggt aaaatagccc tgcattccgc cctgggcagc
      361 gtaaaaggca aagtcgtcag ctgccgaatt caccggttca tccattgcta tcggttttgt
      421 ccatgaaccg taaatcaggc ttgaatacca aatatcaagc cctcccttgc ctccgggcct
      481 gtcggaagca aagtagagcc tcccggaact gtgtaggaat ggatgtatct cggcatcact
      541 gctgttgaca accgggccaa gattttcagg tttggtccag cgcccgttaa cattctcaca
      601 catgtagata tcataactgc ccactcctcc cggcatgtca gaacagaaaa acagatacct
      661 gttgtctgcc gaaacatacg ggaatgacaa gttgtactca gggttattgt attcaaaggg
      721 ggtgatatca gtccattgcg acccggaacg tctggcacta tagatgccga gcctgttggg
      781 ctgttttttc cccagccggc ggccttcgct gttattgcgg gtgaaccaca tagtgttgcc
      841 gtcagttgtc acagaggccg gaccaatgtt tgcatccctg ataagaccgg gtgcgaaccg
      901 gacaggggag tgaggctcac cctcctcgtt gaaaggggca aaataaagct cataaagcct
      961 gactccctca aggtttttct tggtaaagaa tggattggtc ttcctgttgg agcagaacag
     1021 gattccgtca ctcattatca ccggggccat atcatcggaa gaggaactga tttctgagat
     1081 acgcacaacc tcaaaggttc ccttcgcctg tccgctcagg aaagcgatag tgacgaaaag
     1141 gaaaaaaccc gctacagcaa tatgtttcag aattctcacc atgttttcag aaataccttg
     1201 gcgatgttgc ggttacggca aaagcaaact catacctcag tgacacctca tgtgtaccgg
     1261 atgtgtaatt attcagatga ccaagctgat aatcaaatga gtatcctact ttaagctgag
     1321 gtgtgacctg caggtcaagg agggctactg ctgccttctc tgcaattctg aatgaacatc
     1381 caaaccaaag catgtcagca aagatcagat ttgcattcag atcaacttcc aggggctcat
     1441 gcattgaata cctgattagt accgaaggct ttaccttaaa catatcagca accctgacca
     1501 gggtacctgc agtgagatat gtcctgaaca gggaatagtc aggagtcaca ctgaactgat
     1561 cagcctcgtc cctcttatag gtcagcagtg atggtactga caatcccacg aagtattttt
     1621 cagtataata atagaatccg actcccatat tggggagtga gtacttcatg tctcccgaga
     1681 gcagaggatc agcagggtta ccgtcaggga accgcagacg gttgtaattg gtgttgctga
     1741 ggtcaaagcc agccttgagg cccattgaga gctttccatg gccatacctt atgctgtaag
     1801 cgtagaaacc atatataccc acatcctttg tcactccgta tgtgagatag tcagtcatca
     1861 atccgagtga tactctctcc ttcttgttta ccgggctgtg cagtgagagt gtctggaact
     1921 gcggggcgcc gtttatgcgt gcccactgct tgcggtatga gagattggcg ctcagcacct
     1981 cgcgggtacc tgcataagcc ggattgatca ccaggccatt ggtgagatac tggctggtca
     2041 caggcacctg ctgtccgtca accggcaacg caagcaccaa tgcaaggaga actgatatgt
     2101 ataaaccact cttcatctta tctcctgaga atgataaatc cgctgatatg agccgagtat
     2161 tcaggaacat tcaggtcctg cgctcccgtt atggtcaaca ggtaatagta ggttccatcg
     2221 ggtacctccc gtccgttcag gtcaagccct gtccaggcgc caaccggatc attgctgcgg
     2281 taatcatcag ccctgtagac aacagcgcct gcactgttga taatgacaag ctcattgtgt
     2341 gtaaactcca gcccctcaac gttgaagtag tcatttatgc catcattgtt tggtgttacc
     2401 ccctggggta tgatcagatc aggcactgta agtgtcacag tcgcctcagg cgctatgcac
     2461 gcaccgtttg tgaccgcgaa gataaaagtg ttggctccga actccagttt ccttacttcg
     2521 gtgatctttt caagaggtgt tgcaaagacc ggctttgcat tcccgctgct aacatcataa
     2581 cgccataatg aggttccctc accgtcaaaa cttccttcca gaacggctgc cagctgatag
     2641 ggaagcagtg tacggtcagc tcccgcactt atattaacgg gcataatgtc aacattcact
     2701 gtaaccgggg cagatactga aacacaacag ttgttctcac cggagaagac atgcctcctg
     2761 aactgtgttg tttcatcaag catcgcgggc tgatatgatg cagcattgtt ggtgcccggt
     2821 gcagctgtcc attcagatga ctgaggcttt ttcccttccc acaggtacct gtacctgccg
     2881 tcaccgcctg agagtgtgct gccggtaagg gctgccgggg tatgcgtgtt gcaaaccgtc
     2941 tgatcagcag tgatgacatt gccggttatc tgtggcagaa cggtaatccg gaaaactgaa
     3001 ctcaccgagc aggcacctga ggtaacactc ctgcggaacc atgttgttgc cgtaaggttg
     3061 ccatgctggt atgattgacc ggtttcaggg agtggtagga agtaattgtt gtcagttgac
     3121 aaggcccatg cgtatgtgaa tgtacccagt cctcctgctg gagttcccga gaggaggccc
     3181 ggcgctgttc ctgtgcaaat ggtatcatgc ccattcgccg ggaggtttat gtcaaatccg
     3241 gtaatgcgcg gatcaaccgt gatgtgaaca gtgttgctgg tgctggtgca ggcagatgag
     3301 gtcaccacac gcctgtacca gatatcggcg gtaagggtgg tgccgggaag gtcttcagca
     3361 gtagcgccgc tgatattgct gaatgatccc ccgttggaac tgctcatcca ctggtagccg
     3421 taagtgccgg ctccggctcc gttggcagga acggtcccgt caatggttgc cggcgcagtg
     3481 ccttcacaga tggtctggtc agcagatatg acattgttgg ttatcgccgg gtggcagatc
     3541 agggtgacac tgttgctggt cgactggcat gtgttaaggg ggccgctgta aaccaccctc
     3601 ctgtaccagg ttgtgccagg tgaatcattc gtcacgttat aaccggatga ggtagctgat
     3661 cctggtgccg gttgccagtt ggtgttgtca gtgctgctca tccactggta tccgtagctg
     3721 ccggctccgg ctccgccaac ggggttgctg cctgacaggt tgctgaagag agtcccctcg
     3781 catatggtct ggtcagccgt tatattgttg ttggtaatga gaggcaatat ggttactgtg
     3841 acgggtgagc tgacatcaac gcaggctccc gaactgactc tcctgtgata gtgcatcgtg
     3901 gcagtcaggg cctgagggtt ataccttgct gcagtactga caccggcagc attggcccat
     3961 gtaacgttgt cagcgctctg ctcccactgg taaaagtaga tgccattccc tcctgcagga
     4021 cctgaattca gcggatagag ctcctccgga ttcatgttgt agcatagcgt agtgtcattg
     4081 cctatgatat ttccggttat gcgcgggtga acgataatct gcacaggcag gctgcggtca
     4141 atgataccgg cgccgtcact gaccaccctc ctgaaccaga ctgtggctga ttcacttgcc
     4201 gcaggcgtat attcgaagga agtaccgggc acgtttgacc agttaatgtt gtcatagctt
     4261 cgctgccact ggtatgtgta tgtgccactc cctcctgccg gcatcgtacc ggtaagtttc
     4321 gggggaacca tcccttcgca gattgagtca agctggttct ggttataggc tatatatatc
     4381 tcattgaaaa gaaagccgtt aagcggcagg ttaaggttgt ttcgtataag tccgggtaag
     4441 ccgtcacggt tagggtctcc gctgctgtgt atgccaccct gtccgccgtc aaccgttgcc
     4501 gttccgggga atgtcccggc agtccagatc agtccgccgc caccaccacc gccgacgcca
     4561 ttctggttta cattattgcc tcccctgcca ccgtcagcag agagtacagg cgcagaaagg
     4621 aaatgtctgg tgcttattat aaccgaccct ccgccacctc cgccaccggc accggcattc
     4681 agagcggtat tgttatttgc cgctgcgccg tttgcagtga ttattctgcc gttacccaca
     4741 atggtgtcag catgtatgat cacaatacct cccccgtttc caccggctga ggtggtaggc
     4801 gtcgtcaggt aaactgaagc gccgccgccg ccacccatat aaactcccgt attcagtgtg
     4861 gaataactgg caaccgtgaa tcccggccgg cctcccgtcc agagaaaacc acaggttaca
     4921 gaaggcatct cctggtcgcc gacacttccg gaaccgtaat tggaacctcc tccacctccg
     4981 gagaagtgtc cgttgccacc cccgccaccg gtcatattcg cacccttacc cttcatgaag
     5041 gaaggataaa gaggctggcc catggcagta cgtatggcaa gaccttcacc tttgtaccct
     5101 gcaacaactg accatatgtt gtatgattca tacctgagag tatcatgagc attctggcac
     5161 cagcccagtc cctgtgacac gatacctccc ctgaatccgg tcccggcaac atttatattt
     5221 gagttgagcg tcagaactcc ctttaccatg aacaccagaa ccccaccggt gaccgaggct
     5281 gaatccca
//
LOCUS       DAOPBK010000165         5276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13316_length_5276_cov_3.531316, whole genome shotgun sequence.
ACCESSION   DAOPBK010000165 DAOPBK010000000
VERSION     DAOPBK010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13316_length_5276_cov_3.531316"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..343
                     /gene="rpsQ"
                     /locus_tag="PLA17_09185"
     CDS             86..343
                     /gene="rpsQ"
                     /locus_tag="PLA17_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPK85625.1"
                     /translation="MEERNLRKERTGVVVSNKMDKSIVVAVHRKVKHPIYGKFVRKTK
                     KLMAHDEENSCNIGDTVRIMETRPLSKKKSWRLVEIIERAK"
     gene            346..711
                     /gene="rplN"
                     /locus_tag="PLA17_09190"
     CDS             346..711
                     /gene="rplN"
                     /locus_tag="PLA17_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPK85626.1"
                     /translation="MIQQESRLNVADNTGAKEVLCIRVLGGSRKRYASIGDKIVVSIK
                     SALPSGEAKKGTVSRAVIVRTTKEIGRADGSYIRFDDNAVVLLTNQDEVRGTRIFGPV
                     ARELREKYMKIVSLAPEVL"
     gene            730..1053
                     /gene="rplX"
                     /locus_tag="PLA17_09195"
     CDS             730..1053
                     /gene="rplX"
                     /locus_tag="PLA17_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPK85627.1"
                     /translation="MQKKLHIKKGDTVAVIAGESKGQKGRVLKVDRVKSKALVEGINM
                     VSKHTKPNAKAPQGGIVKKEGPIHISNLMVIDPATGKPTRVGRKLNDKNKLVRYAKKS
                     GEEIK"
     gene            1053..1598
                     /gene="rplE"
                     /locus_tag="PLA17_09200"
     CDS             1053..1598
                     /gene="rplE"
                     /locus_tag="PLA17_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPK85628.1"
                     /translation="MYTPALRTKYRNEITPALVKQFNYSTVMQVPRLEKIVINQGLGQ
                     AIADKKLLEMGLKEISDIAGQKAVQTTSRKDISNFKLRKTMPIGVMVTLRHDRMYEFL
                     ERLIAIALPRIRDFKGVSARLDGHGNYTLGITEQIIFPEIDLDKIAKIMGLQITFVTT
                     AKSDEEAYALLKEFGIPFKNK"
     gene            1610..1879
                     /gene="rpsN"
                     /locus_tag="PLA17_09205"
     CDS             1610..1879
                     /gene="rpsN"
                     /locus_tag="PLA17_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HPK85629.1"
                     /translation="MAKESMKAREVKRAKLARKYAAKRAALKAEGNYLALSTLPRNSS
                     PIRQRNRCKLTGRPRGYMRQFGISRITFREMASKGLIPGVRKASW"
     gene            1915..2310
                     /gene="rpsH"
                     /locus_tag="PLA17_09210"
     CDS             1915..2310
                     /gene="rpsH"
                     /locus_tag="PLA17_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPK85630.1"
                     /translation="MTDPIADFLTRVRNAIRAGHKVVETPASGLTKDITRILYDKGYI
                     LSYKFIDDNLQGRIKIALKYHPRTKLPAIKRIQRVSRPGLRRYAGVDELPRVLNGLGI
                     AILSTSHGVMTDKEAVKENVGGEVICYVY"
     gene            2326..2880
                     /gene="rplF"
                     /locus_tag="PLA17_09215"
     CDS             2326..2880
                     /gene="rplF"
                     /locus_tag="PLA17_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPK85631.1"
                     /translation="MSRIGKLPISLPSGVTISVSDDNVVSVKGPLGTLSQKVDSDIKV
                     EVENNTIILARPNDEKRLKSLHGLYRALIANMVTGVSKGYRKDLELVGVGYRAEANGQ
                     RLEMSLGFSHDIIMELPREIKVETKTERRSNPIITLSSIDKQLIGHVAAKIRSLRPPE
                     PYKGKGIKFVGEQLRRKAGKSAGV"
     gene            2901..3260
                     /gene="rplR"
                     /locus_tag="PLA17_09220"
     CDS             2901..3260
                     /gene="rplR"
                     /locus_tag="PLA17_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPK85632.1"
                     /translation="MALAKIEKRKKIKLRIRKRIRGTAEKPRVSVFRSNKQIYAQAVD
                     DVNGVTLASASSREKDIASVTGKKKSEVALLVGKRFAAVCREKGIETVVFDRSGYQYH
                     GRVKSLADGAREGGLKF"
     gene            3266..3781
                     /gene="rpsE"
                     /locus_tag="PLA17_09225"
     CDS             3266..3781
                     /gene="rpsE"
                     /locus_tag="PLA17_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPK85633.1"
                     /translation="MAYGIKRVKNSDLELTDRLVSIQRVTKVTKGGRTFSFSAIVVVG
                     DEKGIVGHGLGKASEVTTAIAKGIEDAKKNLIRVPVHKGTIPHEQYGKYGGAYVFIKP
                     ATEGTGVKAGGAMRAVLESVGVKNVLAKSKGSSNPHNLVKATIAALLELRDASTVADH
                     RGVTLDKVFNG"
     gene            3797..3976
                     /gene="rpmD"
                     /locus_tag="PLA17_09230"
     CDS             3797..3976
                     /gene="rpmD"
                     /locus_tag="PLA17_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPK85634.1"
                     /translation="MAKIKVTQIKSKIGQPESQKRILAALGLRKMHQTVEHEDTPQIM
                     GMVNKLKHLVKVEKL"
     gene            4004..4450
                     /gene="rplO"
                     /locus_tag="PLA17_09235"
     CDS             4004..4450
                     /gene="rplO"
                     /locus_tag="PLA17_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPK85635.1"
                     /translation="MDLSNLKPAPGSVKKEKRIGRGEGSGRGGTSTRGHKGAKSRSGY
                     SRMVGFEGGQMPLQRRVPKSGFKNLNRVDYKAINLSTLEALATKLDTDRIDFETLLTA
                     GLVNKNALVKILGKGKLTRKLEVHAHAFSKSAQASIEAIQGTAVKL"
     gene            4454..>5276
                     /locus_tag="PLA17_09240"
     CDS             4454..>5276
                     /locus_tag="PLA17_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPK85636.1"
                     /translation="MKFIQTIKNIFKIEDLRARILFTLGIVALYRLGKYVTLPGVDPS
                     MLENLRSQTSSGIMGLLDMFSGGAFSQASIFALGIMPYISASIVIQLLGIVFPFFQKL
                     QKEGESGRRKMNQYTRYLTVIILVIQAPTYLANLHAILPPEAFVLQGWFFRLSSVIIL
                     TAGTIFVMWLGERITDKGIGNGISLIIMVGIIARFPVAFIFETGSRMNGSGGAVALLI
                     ELVFLYLVILGSVLLVQGTRRIPVQYARRIVGNKQYGGGRQYIPLKVNAAGVMPII"
BASE COUNT         1579 a         1210 c         1386 g         1101 t
ORIGIN      
        1 agggagagca gaaaaacaat tgccaggctt atgacagaga tgcgcaagag agagatcagt
       61 aagaaaacga aatagccgta tcagtatgga agaaaggaac cttagaaaag aacgtacagg
      121 tgttgtggtc agtaataaga tggacaaatc tatcgtcgtt gccgtccaca gaaaggtcaa
      181 acacccgatc tatggcaaat tcgtcagaaa gacaaagaag ctcatggctc acgatgagga
      241 gaacagctgc aacatcggtg atacggtgcg cataatggag accaggcccc tcagcaaaaa
      301 aaagtcctgg agattggttg aaataattga aagagctaag taatcatgat acagcaggaa
      361 agcaggttga atgtagccga taataccggt gcgaaggagg tgctttgcat cagggtactc
      421 ggtggaagca ggaagagata tgcaagcata ggcgacaaga tagtcgtctc cataaagagc
      481 gctctcccct caggtgaagc aaagaaaggc actgtcagca gggcagtgat tgtaagaacc
      541 accaaggaga taggccgtgc tgacggttca tatatccgct ttgatgacaa tgccgtggtg
      601 ctgctcacca accaggatga agtccgtgga acacgcatct tcggacctgt cgccagggaa
      661 ctgcgcgaaa agtacatgaa gatcgtttca cttgcacctg aagtgttata actgttaatc
      721 tgatacccga tgcagaaaaa attacatatt aaaaaaggag acaccgttgc tgtcattgcc
      781 ggtgaatcca aaggacaaaa gggacgcgtt ctcaaggttg acagggtcaa aagcaaagcc
      841 ctggtggaag ggatcaacat ggtgtcaaaa cataccaaac ccaacgcaaa ggctccccag
      901 ggaggtattg taaagaagga gggccccatc cacatctcaa acctcatggt cattgacccg
      961 gcaaccggta agcctacccg cgtcggacgc aaactgaatg acaagaacaa actggtacgt
     1021 tatgccaaaa aatcaggaga ggagatcaag taatgtatac gcctgcttta agaaccaaat
     1081 ataggaatga gatcacacct gcccttgtga agcagtttaa ttattcaact gtgatgcagg
     1141 tgccccgtct tgaaaaaata gtcatcaacc agggacttgg tcaggccatt gctgacaaga
     1201 aactgcttga aatggggctg aaggagatat cagatattgc cggacagaag gcggtgcaga
     1261 ccacctccag gaaggacata tcaaacttca agctcaggaa gaccatgcct ataggcgtaa
     1321 tggtgaccct gcgccatgac cgcatgtacg aatttcttga gagactgatc gccatagctc
     1381 tgccgcgcat acgtgacttc aagggtgtga gcgccaggct cgacggccac ggcaactata
     1441 cccttggcat caccgagcag atcatcttcc cggagattga cctcgacaag attgccaaga
     1501 tcatggggct gcagatcacg tttgtcacca ctgcaaagag tgacgaagag gcttatgccc
     1561 tgcttaaaga attcggaatt ccctttaaaa acaaataaca gaacggaata tggctaagga
     1621 atcaatgaaa gcccgcgaag tcaaacgtgc gaaacttgcc cggaagtacg ccgccaaaag
     1681 ggccgctctc aaagcagaag gcaattatct tgccctgagc acccttccgc gcaattcaag
     1741 tccaatcagg cagcgcaaca ggtgcaagct cacaggcaga ccgagaggct acatgaggca
     1801 gtttggcatc agcaggatta cattcaggga gatggcttca aaaggactta tcccgggtgt
     1861 aagaaaggca agctggtaac aaccgagata taaacaaatt aatcatacga taagatgaca
     1921 gatccaatag cagattttct taccagggta aggaatgcca tcagagccgg tcataaggtg
     1981 gttgagacac cggcttccgg cctgacgaag gatatcacac ggatacttta cgacaaggga
     2041 tatatcctca gctataaatt cattgatgat aacctgcagg gcaggataaa aatagccctc
     2101 aagtatcatc ccaggaccaa gctccctgca atcaagcgga tacagcgggt cagccgtccg
     2161 ggtctcagga gatatgccgg tgttgatgaa cttccgcgtg tcctcaacgg gctgggaata
     2221 gccattctct caacctccca tggtgtaatg actgataagg aggcagtgaa ggagaatgtc
     2281 ggaggtgaag taatatgtta tgtttactaa acaggaggaa accaaatgtc acgaatagga
     2341 aaattgccta taagcctgcc atctggcgtc accatttcag tgagtgatga caacgtggta
     2401 agcgttaaag gccccctggg gaccctgtca cagaaggttg acagtgatat caaagtggag
     2461 gtggaaaaca atacaatcat actggcaagg cccaacgacg aaaagcgtct taaatcgctg
     2521 cacgggctct accgtgctct cattgccaac atggtgacgg gcgtttcaaa aggctaccgc
     2581 aaggatcttg aactggtagg tgtcggttac cgtgcagaag ccaatggcca gcgtctcgaa
     2641 atgagcctcg gtttttcaca tgatattata atggagctgc ccagggagat caaggttgaa
     2701 accaagaccg aaaggaggag taacccgatc atcactctct caagcattga caagcagctc
     2761 ataggccatg tggcagcaaa gatcaggtca ctgcgtccgc ctgagccgta taagggcaag
     2821 ggtattaaat tcgtgggtga acaactcagg agaaaggccg gtaaatcagc tggtgtgtaa
     2881 catgattaaa tattgaagtc atggctttag caaagataga aaagagaaaa aaaattaaac
     2941 tgagaatccg caagcggatc aggggaacgg ccgaaaaacc aagggtgtcg gttttccgca
     3001 gcaacaaaca gatatatgcc caggctgttg atgatgtcaa tggcgtgaca ctggcttcag
     3061 cttcctccag ggagaaggat attgcctccg taacagggaa gaagaagagc gaggtggctc
     3121 tcctggttgg gaagcgtttt gccgctgtat gcagggagaa gggcattgag accgttgtct
     3181 ttgacagaag cggataccaa tatcatggca gggttaaatc attagctgac ggagcacgcg
     3241 aaggcggctt aaaattctaa tcagtatggc atacggaata aagagagtaa agaacagcga
     3301 tcttgaactt accgacaggc tggtaagcat ccagagagta acaaaagtaa ccaagggagg
     3361 acgcaccttc agcttctcag ccatcgttgt tgtgggtgat gagaaaggta ttgtgggcca
     3421 cggcctgggt aaggcaagtg aggtaaccac cgccatcgcc aaaggcattg aagacgccaa
     3481 gaaaaacctc atcagggttc cggtgcacaa aggtaccata ccccatgagc agtatggcaa
     3541 gtacggcgga gcctatgtct ttatcaagcc tgctaccgaa ggtaccggtg tcaaagccgg
     3601 tggtgcaatg cgcgccgtgc ttgaaagcgt gggcgtgaag aacgtgctcg ccaagtcaaa
     3661 gggttcatca aacccgcaca accttgtgaa ggctaccatt gccgcccttc ttgagctgcg
     3721 tgatgccagc acggtggcag atcacagggg agttaccctc gataaagtgt ttaacggtta
     3781 gtaatattga aatatcatgg caaaaatcaa ggtaacccag attaaaagca agataggtca
     3841 gcctgagtca cagaagagga tcctcgcggc actgggtctg agaaagatgc accagactgt
     3901 tgagcacgaa gatacacctc agattatggg gatggtaaac aaactgaagc acctggtgaa
     3961 ggttgaaaaa ctctaggata ttaacagcat tatatatttc gctatggatt taagcaattt
     4021 gaaaccggcc cccggatcag taaagaaaga gaagcgaata ggacgaggtg agggatcagg
     4081 tcgtggagga acatcaaccc gcgggcacaa aggagccaag tcacgctccg gctacagcag
     4141 gatggttggc tttgaaggag gccagatgcc actccagaga agagtaccga aatcagggtt
     4201 taaaaacctc aaccgcgttg actataaggc tatcaacctc agcacactcg aggctctggc
     4261 aacaaagctt gacactgacc gcattgattt tgagactctg ctgacagcag ggctggtcaa
     4321 taaaaatgca cttgtgaaga tacttggcaa gggtaagctt acccggaagc ttgaggttca
     4381 tgcccacgct ttcagcaaat cggcacaggc atcaatcgaa gcaattcagg gaaccgcagt
     4441 taaactgtaa ggcatgaaat tcatccagac cattaagaac attttcaaga tcgaggatct
     4501 gagagccagg atactcttta cacttggcat cgttgcactc taccgcctgg gtaaatatgt
     4561 caccctcccg ggagtcgatc catccatgct tgagaacctg agaagccaga cctcctcggg
     4621 tattatgggg ctgcttgaca tgttctcggg cggagccttc tcacaggcct ccatatttgc
     4681 cctggggatc atgccctaca tctccgcctc catcgttatc cagctgctcg gtatagtctt
     4741 ccccttcttc cagaaactgc agaaggaggg tgagagcggc aggcgcaaga tgaaccagta
     4801 tacaaggtac cttaccgtta ttatcctggt cattcaggca ccgacatacc ttgcaaatct
     4861 tcatgcaata ctgcccccgg aagcattcgt cctgcaggga tggttcttca ggctctcatc
     4921 ggtgatcatc ctgactgccg gaacaatatt tgtcatgtgg cttggtgaga gaatcaccga
     4981 caagggtatc gggaacggta tctcactgat tatcatggtt ggtattatag cccgtttccc
     5041 ggtggcattc atcttcgaga cagggtcacg catgaacggt tcgggcggcg ctgtagccct
     5101 gctcatagag ctggtattcc tctatctggt gatacttggt tcagtgctcc tggtgcaggg
     5161 gacccgccgc atacctgtcc agtatgcaag acgcatcgtg ggcaacaaac agtacggcgg
     5221 cggaagacaa tatattcccc ttaaggtaaa cgcagctggc gttatgccca ttatct
//
LOCUS       DAOPBK010000166         5217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13503_length_5217_cov_4.284773, whole genome shotgun sequence.
ACCESSION   DAOPBK010000166 DAOPBK010000000
VERSION     DAOPBK010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13503_length_5217_cov_4.284773"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLA17_09245"
     CDS             <1..413
                     /locus_tag="PLA17_09245"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPK85637.1"
                     /translation="KPRLESNTSHKFESHFLGVKITDGKSVMLAGLEGMELGVWVANG
                     EGRFTFPLPEENYNVVLKYSRSHYPANPNGSMYDVAGICSADGRHLAMMPHPERAFMP
                     WQCAYYPADRRNDDMTPWMKAFVNARKWAEGKKK"
     gene            complement(446..997)
                     /locus_tag="PLA17_09250"
     CDS             complement(446..997)
                     /locus_tag="PLA17_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85638.1"
                     /translation="MKRIFFIAVVLVVVVFAGLTSCGNDTSLNSKEGKNRKGTGVEGE
                     AVVLSIGEAELIEVDNNPQYNTAEWLLEVEKPGRWDVWLSSLTVDTTKLHFSESVIIT
                     AGETKLEKRPLSDRIVHEDKSVTEPWYRADSHMGSVFFSKPGEYHVQVISDKVEAHST
                     DLSQVSIDKHTLINSVILKPKVN"
     gene            complement(1128..3035)
                     /locus_tag="PLA17_09255"
     CDS             complement(1128..3035)
                     /locus_tag="PLA17_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPK85639.1"
                     /translation="MNFELFTLPFNLGLYFILIYSVVRITIWFRDLSRSDKLRLQRGF
                     FGRPFMDSLREIFMESLLHRKIFRKNPVLGYMHMSLAFGWFLLIVFGTFEADFFGEKH
                     LNPPSHAIFFRWFNPEHGAEGFARIYSIWMDLLLAFILSGLLLAIIKRFTPRLMGMKK
                     TTKHTWTDRIALISLWLIFPSRLLAESFTCGAHGSGSFLTGNLGNALASFLPVADIAP
                     WFWWLYSLSLGVFFFLLPISRYFHIPVELFLIFMRNSGIITGDRHSSYTEVQTHACSS
                     CGICISTCQMSYAAGIHDIQSAYFIKGLRNDRDIAAITANCLMCGRCEEVCPVGIELG
                     PMRLIERRQGERSDRTLKLKEKYSLMRRRSTIEKRPTQSYSYLVREEPEKADVLFFAG
                     CMTHLTPAVIAAMKKIMDSAGVRYRFMDENGGVCCGRPLMLAGRDREARELINHNSDI
                     IWKSGAQILVTSCPICYKVFRESYYLEARVMHHSEFINLLIEEGSLRLGYLHKKVAYH
                     DPCELGRGSGIYEEPRAVISYVAELQSSAQEANMSLCCGGSLGNLTLSSGKRAMIAAD
                     AVRTLTLCSPDELITACPLCKKTFAQVTKTKISDIAELVAAAIPAPKREIAKRRVVRR
                     YSGASAMVEKK"
     gene            complement(3025..3384)
                     /locus_tag="PLA17_09260"
     CDS             complement(3025..3384)
                     /locus_tag="PLA17_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPK85640.1"
                     /translation="MRVTQSNKFGFTINRSRLIDYDSSDRKVREYVTLREPSFRLCFA
                     CGGCTATCTAGQHTSFNLRRMNTFIRRGEIEPLREEVKRCMLCGKCLLVCPRGVNTRN
                     VVAAAAEAIAKLERNEL"
     gene            complement(3381..4403)
                     /locus_tag="PLA17_09265"
     CDS             complement(3381..4403)
                     /locus_tag="PLA17_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK85641.1"
                     /translation="MSAHIVVAGGGVAGMEAAGTLAGMGYRVTMIEKDDAPGGHLLQW
                     HALFPDRRPADEVISHFRKHTEHPNLRIITGTTVEKIRNHGSGVLVSLSGGAEEQADA
                     VIVATGFELFDAHRKEEYGYGIYDNVITSADLEAMFNRGLVAKADGSVPSRIGLVHCV
                     GSRDEKVNNNHCSKVCCVTAVKQAIEIRKMLPSCEVFCFYMDMRMYGPYYEELYRESQ
                     EYHNVNFIRGKVSEASVNINNRLVIKVEDTLAGRPLKMELDIIVLMVGMEMAGAGMRL
                     AEASGMKRGINRFLESADHHYGNNLSGLRGVFYAGTCTAPMNITDTISHARAAVTETV
                     KYLNSR"
     gene            complement(4409..>5217)
                     /locus_tag="PLA17_09270"
     CDS             complement(4409..>5217)
                     /locus_tag="PLA17_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPK85642.1"
                     /translation="ALATEAGYKHIAISCVTSFGIYSEILETWKHFPETLERTRENLY
                     KATGRTFDVPETISHTSDIIYKFRDQIARKAKYHLIDISTGEPLKVVEHIGCHYSKMF
                     PKYGVGGAEFPHVLVGMVEAWGGDIIDYPERRHCCGFGFRQYLVQANRGYSLFATRKK
                     FESMQPYRPDMIIANCPGCTMFMDRWQYTIREIDGITYGADGQGIPVLTYEEVAGLVL
                     GYNPWELGLQYHNVQSLPLLRKMGIKADPSERFLAKDGSRLPEPENLING"
BASE COUNT         1254 a         1435 c         1248 g         1280 t
ORIGIN      
        1 agaagccgcg gctggaaagc aatacttccc ataaattcga atcacacttc ctgggtgtga
       61 agattacaga cggcaaatcc gttatgctgg ccggcctcga gggaatggag ctgggagtat
      121 gggtggccaa tggtgaaggc cgctttacct tcccgctgcc tgaggagaat tataacgtcg
      181 tgttgaaata cagccggagc cactatccgg caaatcccaa tgggtcaatg tacgatgtgg
      241 caggtatctg ctctgctgac ggcaggcacc tggcaatgat gccccatccc gagagggcat
      301 tcatgccatg gcagtgtgca tactaccccg ctgacaggcg caatgatgac atgacgccat
      361 ggatgaaggc attcgtcaat gcccgcaaat gggccgaggg aaagaaaaag tgaaggtggc
      421 agcgccaccg gcgccggaac aacggtcagt tgaccttggg cttcagtatc actgaattga
      481 taagggtatg cttgtcgata ctgacctgtg aaagatcagt agaatgagcc tctaccttgt
      541 cactgataac ctgaacatgg tattcaccgg gttttgaaaa aaacacagac cccatatgtg
      601 agtcagccct gtaccagggt tcggtaacac tcttgtcttc atgaactatc cggtcactga
      661 gcggcctttt ttcaagcttg gtctcaccgg ctgtaataat tacactttct gagaaatgaa
      721 gtttggtggt gtcaactgtc aggcttgaaa gccagacatc ccagcggccg ggtttctcaa
      781 cctctaaaag ccactcggca gtattgtact gcggattgtt atcaacctct atgagttctg
      841 cctcacctat actcagaaca acagcctccc cttccacgcc ggtccctttt ctgttctttc
      901 cttccttgct attgaggctg gtgtcattcc cgcatgaggt aagaccggca aataccacga
      961 caaccaacac gactgcaata aaaaaaattc tcttcattct actattaaaa aattgtcaat
     1021 taggaaccag caaaaactat tccagagtta tcctgaaaaa ttatcgtgca aagtaaatgg
     1081 aaataactga aatacgaaat atcaatgatt caaatatttg caaactttca ttttttttca
     1141 accattgctg atgccccgga gtatcttctt acaactctcc tttttgcgat ctcccttttt
     1201 ggtgccggga tggcagccgc caccagctcg gcaatatcgc ttattttagt tttagtgacc
     1261 tgagcgaagg ttttcttgca cagcggacag gcagtaatca gttcatcagg tgaacagagg
     1321 gttagcgtgc ggacagcatc ggctgctatc atagccctct tccctgatga cagggtgaga
     1381 ttgcccaggc tgccgccaca acagagcgac atgttcgcct cctgcgctga tgactgcagc
     1441 tctgccacat agctgataac cgccctgggc tcttcgtaaa tgcctgatcc gcgtcccagc
     1501 tcgcacgggt catggtatgc caccttcttg tgaaggtaac cgagacggag tgagccctct
     1561 tctatgagca ggttgataaa ctccgaatga tgcatcaccc gggcttcaag atagtaactc
     1621 tccctgaaaa ccttgtagca gatggggcat gacgtaacca gtatctgcgc tccggacttc
     1681 cagataatgt ctgagttatg gtttatcagc tcccgtgctt ccctgtccct tccggcaagc
     1741 atcagcggcc ttccgcagca gacccctccg ttttcatcca tgaaccggta tctgaccccg
     1801 gcactatcca tgatcttctt catggcagca atcactgcgg gggtcaggtg ggtcatacat
     1861 ccggcaaaaa agagtacgtc agccttctca ggctcctccc tgacaagata tgagtatgat
     1921 tgtgtcggtc tcttctcaat ggtgcttctc ctgcgcatca gcgaatattt ctccttcagc
     1981 ttcagagtcc tgtcactcct ctctccctgc ctccgctcaa tgagcctcat gggaccaagt
     2041 tcgatgccca cagggcatac ctcctcacac cttccgcaca taaggcagtt ggctgtgatg
     2101 gccgcgatgt cacggtcatt cctcagaccc ttgataaagt aagccgactg tatatcatgt
     2161 atcccggcag catagctcat ctggcatgtg ctgatgcata taccgcatga tgaacatgcg
     2221 tgtgtctgca cctcggtgta cgatgaatgc ctgtcgcctg tgataatgcc ggagtttcgc
     2281 atgaagatca ggaaaagctc caccggtatg tgaaaatacc tggaaattgg cagcaggaag
     2341 aagaatactc cgagagagag ggaatagagc caccagaacc agggcgcaat atctgccaca
     2401 ggaaggaatg atgcaagggc attgcccagg tttccggtaa ggaagctgcc gctgccgtgc
     2461 gccccgcagg tgaaactctc tgccagcagc ctcgagggga aaatcagcca gagactaatg
     2521 agagctatac gatcagtcca ggtatgcttc gttgtcttct tcatccccat caggcgggga
     2581 gtgaacctct tgataatagc cagcagcagt cctgaaagga tgaatgccag cagcaaatcc
     2641 atccagatac tgtatattct tgcgaatccc tcagccccgt gttcagggtt aaaccagcga
     2701 aagaatatgg catgcgaggg cgggttgaga tgcttctccc cgaagaagtc agcctcgaag
     2761 gtaccaaaga ctatcaggag gaaccagccg aaggcaaggc tcatatgcat ataccccagc
     2821 accggatttt ttctgaaaat cttgcggtgc aaaagactct ccatgaatat ctccctcagg
     2881 ctgtccatga acggcctccc gaagaaaccc cgctgcagac gcagtttgtc tgaacgggag
     2941 aggtcacgga accatatggt aatccgcact acactgtaaa tcaatatgaa atacagtccc
     3001 agattgaacg gtaatgtaaa caactcaaag ttcattccgt tccagttttg ctattgcttc
     3061 ggcagccgct gccaccacat tgcgtgtatt taccccccgg ggacagacaa gaaggcattt
     3121 gccacagagc atgcagcgct tcacctcttc acggagaggt tcaatctctc ccctcctgat
     3181 aaaggtattc atccgtctca ggttaaatga ggtatgctgg cccgctgtgc atgtcgcagt
     3241 acacccgccg caggcaaagc agagcctgaa acttggctcc ctcagtgtaa catattctct
     3301 tactttcctg tcagaggagt catagtcaat cagccggctc cggttgatgg taaatccgaa
     3361 cttatttgac tgagtgaccc tcatctgctg ttaagatatt ttactgtttc cgtcactgcc
     3421 gccctcgcgt gcgaaatggt atcggtaata ttcatcggag ccgtgcaggt gcctgcgtaa
     3481 aagacccccc ttagcccgct gagattgttt ccatagtggt gatcagcgct ctcgaggaac
     3541 cggttgatcc ctctcttcat cccggaggct tctgccagcc tcatcccggc cccggccatc
     3601 tccattccaa ccatcagtac aatgatgtca agctccatct tcagcggcct gcctgcaagg
     3661 gtatcctcca ccttgatcac cagcctgttg tttatattta cggatgcctc tgacaccttg
     3721 cccctgatga aattgacgtt gtgatactcc tgtgactcac ggtaaagctc ctcataatag
     3781 ggaccgtaca tgcgcatatc catgtagaag cagaagacct cgcacgaggg aagcatcttc
     3841 ctgatttcta tagcctgttt gacggcggtc acgcagcata ccttggagca gtggttgttg
     3901 ttcaccttct cgtcacgtga tcccacgcag tgaacaagcc cgatacgcga aggcacactc
     3961 ccgtctgcct ttgccacgag gcccctgttg aacatagcct ccagatctgc cgaagtaatg
     4021 acattgtcat agatcccgta tccgtactcc tctttcctgt gggcatcgaa gagttcaaac
     4081 ccggtggcca cgatcaccgc atcagcctgc tcttcggccc ctccggagag actgacaagc
     4141 acaccgctgc catgattcct gatcttttct accgttgttc cggtgatgat ccgcaggttg
     4201 gggtgctcag tatgttttct gaaatggctt atcacctcat ccgccggacg gcgatcagga
     4261 aagagtgcat gccactgcag cagatgcccc ccgggtgcgt catccttttc gatcatggtg
     4321 acacgatagc ccattcctgc aagggtgcct gctgcttcca tgccggcaac accgccgccg
     4381 gctacaacta tgtgtgctga catggtcttc atccgtttat aagattttca ggctcaggca
     4441 gcctggagcc atccttcgca aggaagcgct ctgacggatc tgccttgatg cccatcttgc
     4501 ggagcagggg aagcgactgc acattatgat actgaagccc gagctcccat ggattgtatc
     4561 ccagtaccag accggcaacc tcctcgtagg tcagaacagg aatcccctgg ccatcagcac
     4621 cgtaggtaat accgtcaatc tccctgatgg tgtattgcca gcggtccatg aacatggtac
     4681 agccagggca gttggcaatt atcatatcgg gcctgtaagg ttgcattgac tcaaacttct
     4741 tacgggttgc aaagagcgag tatcctctgt tggcctgcac caggtactgt ctgaatccga
     4801 atccgcaaca gtgacgcctt tccgggtaat caatgatatc accaccccat gcttcaacca
     4861 ttccaaccag cacatgcggg aattcagccc cgcccacccc gtacttcggg aacatcttcg
     4921 agtaatggca tcctatgtgt tcaaccacct tcaaaggctc accggtgctt atgtcaataa
     4981 gatggtactt tgccttgcgg gctatctgat ccctgaactt atagatgatg tcactggtgt
     5041 gcgaaatggt ttcaggaaca tcaaacgtgc gccctgtggc cttgtacaga ttttcacgcg
     5101 tacgttcgag cgtttccggg aagtgtttcc aggtctcgag tatctctgag tagataccga
     5161 aagaggtgac gcatgagatt gctatatgct tgtacccggc ttcggttgca agcgcaa
//
LOCUS       DAOPBK010000167         5190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13583_length_5190_cov_5.330867, whole genome shotgun sequence.
ACCESSION   DAOPBK010000167 DAOPBK010000000
VERSION     DAOPBK010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13583_length_5190_cov_5.330867"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..2467
                     /gene="gldG"
                     /locus_tag="PLA17_09275"
     CDS             65..2467
                     /gene="gldG"
                     /locus_tag="PLA17_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HPK85643.1"
                     /translation="MFTLFRKETAAFFSSLTGYIVMIVFLLANSLIMWIMPGQWNLLD
                     SGYAGLDPLFVLSPWIFLFLVPAITMRLFSEERKSGTIELLWSRPVSDGAVIYGKFLA
                     ALFLVIMALLPALVFVISVWVLGENPGNLDRGGTAGSFTGLLMLAAVYAAIGLFASSL
                     TSNQVISFIIAAVLSFLMFRGFDSLATLPALSSVAEEVSLMGINEHYRSISRGVLDIR
                     DIAYFVFVAVFFCEAARVSLRRSSIRKPLIVVTATGAVCILLSLLHIRADLTEEKRFT
                     LAEPTKKILRELGSDVTVNVYLDGELPIAFRKLRRSTEQYLDEFRVTSGRKVKYDFIN
                     PSASSDRTERDRVHAELMNRGLNPVNVMASDGEGGRTQKRIFPSLTVTSGTTVIPVNF
                     LRNNPTLPAETNLLHSTEGLEYEIIQAVAAATADTVHTVAFIEGHGEFDEIFVADLTL
                     ELAKFYNIDRGVIGGRRGSIDKYAAIIIAAPQKPMDERDKFVIDQYIMNGGKVLWLAE
                     EVKVDADSLIAGSTVALYEPLSTADMLFRYGVRINPVIVQDADCMLIPLKVVNPGGEV
                     QYVPTPWVYYPLLRPSQTHPITRNLNRVKSEFAGTIDTVGRDPGVKKSILLTTSPEAR
                     TVMPPLVISLEEADEMIPDGMLNAGPLNVAVLLEGTFSSVFANRMAGSIMGDPQLVTV
                     TESRPTKMIVVADGDIIRNEVSWGGGNPEPLTLGLDRYTMQTFGNKDFLVNCINYLVD
                     ENELIGMRSRELKPRLLDQTRIKEQRTLWQVVNTLLPILLIIIAGVIWNALRRRRYIS
                     LK"
     gene            2594..3724
                     /gene="dnaN"
                     /locus_tag="PLA17_09280"
     CDS             2594..3724
                     /gene="dnaN"
                     /locus_tag="PLA17_09280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPK85644.1"
                     /translation="MKFVVSSTELLGHLQAIGRVISSKNTLPILDNFLFNISGNDLEI
                     TASDLESTLITRMHLDNISGSGMIAIPARILLDTLREFSEQPLTFDINTDTMAIVINS
                     ENGQFSIVGQNGIDFPVLPVIKQEKRFGFNVSAEVLLAGISRTLFATADDELRPVMGG
                     ILVEASTANLTFVASDAHKLVRYQRSDARADDNASFILPKKPASLLKNILPRESGVVS
                     VEFDDRNAFFNLSDYKVVCRLVEGNYPNYNSVIPKNNPRRVRIDRVEFYNILRRVSVF
                     SNQASNLVKLQFTPNQITVSAQDVDFSISAYERHKCLYEGDDMEIGFKSVFLIEILSN
                     IASQDVVIELADPTRAGLFLPSETANEDEDLLMLLMPMMINA"
     gene            3738..4508
                     /locus_tag="PLA17_09285"
     CDS             3738..4508
                     /locus_tag="PLA17_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPK85645.1"
                     /translation="MELNLRKPLVVLDLETTGVSIASDRIVEFSALKVSPGGAEEWLS
                     MRLNPGIPISPEATRVHGITDADVANEPHFRDVARRIAAFLEGCDLAGFNSMKFDIPI
                     LCEEFLRVNVDFNPARHRYVDVQVIFHKKEQRTLSAAYKFYCDKELENAHSSKADAAA
                     TYEILKAQLDRYPDLENDIGKLSSYSAFNNNADLAGRIIFNEQGQEVFNFGKHRGKPV
                     EQVFREEPSYYSWMMNGDFPLNTKQVITAIKVRSFGKA"
     gene            4515..5126
                     /locus_tag="PLA17_09290"
     CDS             4515..5126
                     /locus_tag="PLA17_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPK85646.1"
                     /translation="MKIICVGLNYRRHAAEMGRPIPPEPMIFLKPDSALLKRNKPFFI
                     PDFSNNLHFEAELVLRISKLGKGIAARYAYRYYDALTVGIDFTARDLQHRFSQAGHPW
                     ELSKTFDGSAPVGTFIEAERFPDHSDISFSLEINGELRQEGNTSDLIFGFAEIIEYVS
                     KYYTLKTGDLIFTGTPSGVGPVKKGDNLVARLAGETVLDFFVR"
BASE COUNT         1279 a         1311 c         1339 g         1261 t
ORIGIN      
        1 aaaaatgccc gcaggccggt cagaccatga aagttttgga tatttttaca gcaaacttga
       61 agttatgttt actcttttca gaaaagagac agcagcgttt ttcagctctc ttacgggtta
      121 tatcgtaatg attgtgtttc ttctggccaa cagcctgatc atgtggatca tgcctggcca
      181 gtggaacctg cttgacagcg gctatgccgg ccttgatccc ctttttgttc tctctccgtg
      241 gatattcctt ttcctggtgc cggcaatcac catgaggctc ttttccgaag agagaaagtc
      301 gggaaccata gaactgctat ggtcgcgccc ggtgagtgac ggtgccgtga tctacggcaa
      361 gttccttgca gcgctcttcc tggtgataat ggccttattg cctgcactgg tgttcgtcat
      421 ctccgtgtgg gtgctgggtg aaaaccccgg taaccttgac aggggaggta ccgccggatc
      481 attcacaggc ctcctgatgc ttgcagcagt ctacgccgcc atcggcctct ttgcctcatc
      541 actcacctca aaccaggtga tctcattcat aattgcagcc gttctctcct tcctgatgtt
      601 caggggattt gactcgctgg caaccctgcc cgccctctca tccgttgctg aggaggtttc
      661 cctgatggga atcaatgaac actaccgctc aataagcagg ggggtgcttg atatccgtga
      721 catagcctac ttcgtattcg ttgccgtatt cttctgcgag gctgccaggg tatcactccg
      781 ccgcagcagc atcagaaaac ctcttatcgt ggtgacggca accggagcgg tctgcatact
      841 tttatcgctc ctgcacatca gggctgacct gacggaagag aagcgcttca cgctggcaga
      901 accgacaaag aagatactca gggaactcgg gagcgatgtg acggtgaatg tttacctcga
      961 cggcgaactg ccgatagctt tcaggaaact gcggcgcagc acggaacagt acctcgatga
     1021 gttccgtgtt acatcaggga ggaaggtgaa gtatgacttc atcaatccct cagcctcttc
     1081 agacaggact gagcgcgacc gggtccacgc tgaactcatg aaccgcggcc tcaacccggt
     1141 aaatgtcatg gcctccgacg gtgagggagg aagaacacag aaacggatct tcccttcact
     1201 taccgtcacc agcggcacta cggtcatacc ggtgaacttc ctcaggaata atcccaccct
     1261 gcctgctgag acaaatctcc ttcattcaac cgaagggctt gaatatgaga ttattcaggc
     1321 agttgccgcg gcaactgccg atacggtgca cacggtggca ttcattgaag gtcatggtga
     1381 gttcgatgag atcttcgtgg ctgatctgac cctggagctg gctaagttct acaatattga
     1441 caggggggtg atcgggggca ggaggggatc cattgataaa tatgccgcca taatcattgc
     1501 cgcgccgcag aaaccgatgg atgaaaggga taagtttgtg attgaccagt atatcatgaa
     1561 cggtggaaag gtgctatggc ttgcagaaga ggtgaaggtg gatgcagaca gcctgattgc
     1621 aggatcgacg gtggcgctgt atgagccact ctcaacagca gacatgctct tcaggtacgg
     1681 tgtccgcatc aacccggtga ttgtgcagga tgccgattgt atgctgattc ctctcaaggt
     1741 tgtcaatccc ggaggcgagg tgcagtatgt gcccacaccc tgggtctatt acccgctgct
     1801 gcgcccttca cagactcacc ccatcacacg taacctcaac agggtcaagt cagagtttgc
     1861 cggcaccatt gacacggtgg gacgcgatcc gggggtgaaa aagagcattc tccttacaac
     1921 atcgcccgaa gcccgtacag taatgccacc gctggtcatc tcactggagg aggctgatga
     1981 gatgatccct gacgggatgt taaacgccgg ccccttgaac gtggcagtgc tccttgaagg
     2041 aaccttcagc tctgtcttcg ccaaccgcat ggcaggcagc ataatgggtg accctcagct
     2101 ggtgactgtg acggaaagca ggcccactaa gatgatagtg gttgctgacg gtgatataat
     2161 acgcaatgag gtctcatggg gaggcggcaa tcccgaaccg ctgacactcg gccttgacag
     2221 atacaccatg cagaccttcg gcaacaagga cttccttgtg aactgcatca attacctcgt
     2281 tgatgaaaac gaactcatcg ggatgcgttc acgggaactg aagcccagac tgctcgatca
     2341 gacccgtata aaggagcaga gaacactctg gcaggttgtg aacacgcttc tgcccatatt
     2401 gctgattatt atcgcgggag tgatatggaa cgcactcaga cgcaggcgtt acatatcatt
     2461 gaaataaatg tttgagggta acactttttt ttgtaatatt gataatccgg tttttaaaac
     2521 agatattaat tagtaatcag ttgattgccg gtaaactgag atatattgcc gaataaatta
     2581 aacagaccga aatatgaaat ttgttgtttc aagcacagaa cttctgggtc acctgcaggc
     2641 cattggcagg gtgatcagtt caaagaatac tcttccgatt cttgacaact tccttttcaa
     2701 cataagcggg aatgatctgg agataacggc ttcagacctc gaatcgacgc ttatcacacg
     2761 catgcatctt gacaatatct caggaagcgg aatgatagcc attcccgcca ggatactgct
     2821 tgacaccctc agggagtttt ccgaacagcc gctcaccttt gatatcaata ctgacaccat
     2881 ggccattgtc atcaattcag agaacggcca gttcagcatt gtgggtcaga acgggattga
     2941 ctttcccgtg ctgccggtca tcaagcagga gaagcgtttt ggcttcaacg tatctgcaga
     3001 ggtgctcctg gcaggtatct caagaacact gtttgcaaca gctgacgatg agcttcgtcc
     3061 cgtaatgggt ggcatacttg tggaggcatc aaccgccaac ctgacattcg ttgcatctga
     3121 tgcgcataag ctggtcagat accagagaag tgacgcaaga gctgacgata atgcatcatt
     3181 catccttccc aagaagcctg cctcactgct gaagaatata cttcccaggg agagcggcgt
     3241 ggtatcggtt gagttcgatg acagaaacgc atttttcaat ctatctgact acaaggttgt
     3301 atgccgcctg gttgaaggca attaccccaa ctacaactcc gtcatcccga agaataatcc
     3361 caggagggtc cgcattgaca gggtcgagtt ttacaacatc ctgcgcaggg tctccgtatt
     3421 ctcgaaccag gcaagcaacc tggtcaagct gcagtttacg cctaaccaga taaccgtctc
     3481 agcccaggat gtcgacttct caatctcggc atacgagcgt cacaagtgcc tctatgaggg
     3541 tgatgacatg gagataggct tcaagtcggt cttcctgatt gagatacttt caaacatcgc
     3601 atcacaggac gtggtgattg agcttgccga ccccaccagg gccgggctct tccttccctc
     3661 cgagacggcc aacgaggatg aggatctcct catgctcctg atgccgatga tgataaacgc
     3721 ctgagtttca gtaaaaaatg gagcttaacc tcagaaaacc cctggtcgtt cttgatcttg
     3781 agaccaccgg ggtgagtatc gcatctgacc gtatcgtgga gttttctgct cttaaggttt
     3841 cgccgggagg cgcagaagag tggcttagca tgagactgaa cccgggcata cccatatcac
     3901 ccgaggcgac acgcgttcac ggcatcactg atgccgatgt tgccaatgag cctcacttca
     3961 gggatgtggc aagaagaatt gcagctttcc tcgaaggttg tgaccttgcc ggattcaact
     4021 caatgaagtt tgacataccc atactctgcg aggagtttct ccgggtgaat gttgatttca
     4081 accctgcccg gcacagatat gtagacgtgc aggttatttt tcacaagaag gagcagagga
     4141 ccctgagcgc tgcctacaaa ttctactgtg acaaggagct tgagaatgcc catagctcaa
     4201 aggctgatgc tgctgccaca tatgagatac tgaaggcgca gctcgacagg tatcctgacc
     4261 tggaaaatga tattggcaag ctctcttcat acagtgcatt caataacaac gctgaccttg
     4321 caggaaggat aatcttcaac gaacagggac aggaggtttt caacttcggc aagcacaggg
     4381 gcaagcccgt tgaacaggta ttcagggagg agccttcata ctactcctgg atgatgaatg
     4441 gcgattttcc cctcaacacc aagcaggtga taactgccat caaggtgcgt tcattcggta
     4501 aagcttagtg tttcatgaaa ataatatgcg ttggacttaa ctaccgcagg catgcggcag
     4561 agatgggtcg tccgataccc ccggagccga tgatcttcct aaagcccgat tcggctctgc
     4621 tgaaaaggaa caagcccttc tttatccctg acttctcaaa taacctgcat tttgaagcag
     4681 agctggtact caggatctcg aagctgggga agggcattgc tgccaggtat gcttaccgct
     4741 attatgatgc actgactgta ggtatagact tcaccgcgcg cgacctgcag cacaggttct
     4801 cacaggccgg acacccgtgg gagctctcaa agacgtttga cggctcggcg cctgtcggta
     4861 ctttcattga ggcagaacgg ttccctgatc attcagatat cagcttttct ctggagataa
     4921 acggggagct gaggcaggag gggaacacat ccgatctgat tttcggtttt gcagagataa
     4981 ttgaatatgt ctcgaaatat tacactctca agacgggtga cctgattttc accggcacac
     5041 cctcaggtgt tggccccgtc aaaaagggag acaatcttgt ggcccgcctg gccggtgaga
     5101 cggtgcttga cttttttgtc aggtgagcag ttgatgcagt tatatatggg cctgcaatta
     5161 atgcttttcc tgtctggcat gttctggctt
//
LOCUS       DAOPBK010000168         5148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13705_length_5148_cov_5.022187, whole genome shotgun sequence.
ACCESSION   DAOPBK010000168 DAOPBK010000000
VERSION     DAOPBK010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13705_length_5148_cov_5.022187"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2358
                     /locus_tag="PLA17_09295"
     CDS             <1..2358
                     /locus_tag="PLA17_09295"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPK85647.1"
                     /translation="RHPLLQNTSFAVAATNPRDTVIYLINDDHEAFAYDRGQLISLNE
                     SNVQKITMTDGQIRYKYMSDPDFLTIEPFYWETGNLALYYEDREGTIWAGTETSIYRL
                     MSQAFVIYEREHGLPEETWAVVADPHEGIWTGSVEGELRYFDGEKFHVRNEYQALLGQ
                     PVSFYRGSATLSNGEIWFSTREGVLIWDGKRFRVPKLCPNNYQVCIIWEDPVDKSVFV
                     GTDRGLFHIKEDKVTIHEEMSWYGHGIVEGVARDHEGNYWLAGHHGMVYFDGKEFTPF
                     RSEPAPVEMIWGVVCDHMGNIWSAGSDGLHICNPNNPVFDNALPEEVNLPASVIRDLG
                     NHKLIVGRMMDICIIDLNKYYSGQPDYYSIIGRSQGFPGNDCQDNGIMQDKEGRWWIL
                     TSDKLICFDPDKISRNTHPPMNHITKVEIQGKEAPWETALEASLFYDTTSFLKIKGKQ
                     NRIRISYTGISTRNPEGVRYRYRMVGFNDNWSEKTTERSVVYSNLPPGEYLFRVQAIN
                     ADGVLSEEPDQLTISIVPTFFQSKIVNAIMMMLALSLMVFLSFQIRKAVLVKRVTTAR
                     REAEAYRLQLNSVIRQFDPHFTFNAVTSVGSLIMKGEKEKAYNYFIKLSNLLRSIISD
                     SNVLLRPLEDEIEFVTRYCELQKLRFGNRFEYTVNIDSDVNLKSPVPKMIIQSFVENA
                     IKHGLENKKEQGILRISISNCGAGMEVIILDNGIGRAAAADLQTHGPGTGLKNIAGIV
                     ETLNKVNNEKITFTLTDLHDNGRASGTKVTVFLPYNYSLDLTADM"
     gene            2386..3150
                     /locus_tag="PLA17_09300"
     CDS             2386..3150
                     /locus_tag="PLA17_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPK85648.1"
                     /translation="MNSRKIRSMIVEDEPEALDLLTGLLEATGEATVVASTTNPFEAI
                     DLLLLHTPDILFLDIKMPGMSGFDIINEMNRLTALTPHVVFTTAHDEYAIKAFDCAAF
                     DYLLKPIDPERLAETLHRHSSQTVRKKREQLQQQSFPNNEKILIFRGVTGVTFIDTDE
                     VVYITADGNYSSFHFISGRIETVTSLIGNIEAQLGKQFFRTSRSCIINTSYLARIDMK
                     QLSCVFIKGEKEFRCEISRDKVKMLTDFMKNRFFEL"
     gene            complement(3402..4874)
                     /locus_tag="PLA17_09305"
     CDS             complement(3402..4874)
                     /locus_tag="PLA17_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPK85649.1"
                     /translation="MKSIFRSVMRWMRACLPAVVVAALFAGPLVAQDHDERYIPESDP
                     LVLRKLEEWQDLKFGLLMHWGPYSQWGIVESWSICAEDEGWCRRKNPDYTEYKKQYEA
                     LKLSFNPIAFDPEKWAAAARNAGMKYVVFTTKHHDGFSMFDTKLTDYRITDPECPFSV
                     NPRANVTKEIFRAFREQGLWTGAYFSKPDWHSEYYWWPNFATPDRHVNYSITDYPERW
                     EKFVEFTHGQIMELLGGDYGTIDILWLDGGWVRKMTDDEVLRSRMAQGNKMTRVQSQD
                     IRMDELVAKARQAQPGLIVVDRAVYGKNQNYLTPENRVPEKALPYPWESCIIAGGGWA
                     WVPDPKFMSPREAVHLLVDIVVKGGNLLLNIGPAPDGTWPAEAYSLLEEIGRWIKVNG
                     EAIYGTRALAPHKDGKSCITRKGDDTWYLYYMADEGEQMPAAITMNGLTLPAGAKVSV
                     PGSGAVCRWKNGNGSFTVSVPHHVRNRPSSDYVWVFRIEI"
     gene            complement(4886..>5148)
                     /locus_tag="PLA17_09310"
     CDS             complement(4886..>5148)
                     /locus_tag="PLA17_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85650.1"
                     /translation="TMSRDSTILYLFFPGNGGGELMLEGVKNRINKAFVVGNGTNLEV
                     KEYLRPYWSDHAGVYFIRVPEETLDKTMTVVGISLNGKLETD"
BASE COUNT         1357 a         1342 c         1239 g         1210 t
ORIGIN      
        1 cgccatccgc tactccaaaa tacaagcttt gcagtagcag caaccaatcc acgtgacacg
       61 gtcatctatc tgataaatga tgaccacgaa gccttcgcct atgacagggg tcagctgata
      121 tcgctcaatg aaagtaatgt ccaaaaaatt accatgaccg atggccagat caggtacaag
      181 tatatgtcag accccgactt ccttaccata gaaccattct attgggaaac cggaaacctg
      241 gcattgtact atgaggaccg ggaagggaca atctgggcag gcacggagac cagtatctac
      301 aggcttatgt cacaggcatt cgtgatatat gaaagggaac acggattgcc tgaggagacc
      361 tgggctgtgg tagctgatcc tcatgaagga atatggaccg gctctgttga aggtgaactg
      421 agatactttg acggagaaaa attccacgta agaaatgaat accaggcact gctcggtcaa
      481 ccggtatcct tctacagggg aagcgccacc ctgagcaacg gcgagatatg gttttcaaca
      541 agagagggtg tgcttatctg ggatggcaaa aggttcagag taccaaagct atgtcccaat
      601 aattaccagg tctgcattat ctgggaagac cccgtggata aaagcgtttt tgtcggcact
      661 gaccggggcc tcttccatat caaagaagac aaggtcacca tccatgagga gatgtcatgg
      721 tacggacacg gaatagtgga gggtgtcgca agggatcacg agggcaatta ctggctggcc
      781 ggacaccacg gcatggttta cttcgacgga aaagaattta ccccgttcag gtcagagcca
      841 gccccggttg agatgatatg gggtgtggtc tgcgaccaca tgggaaacat ctggagtgcg
      901 ggttctgacg gcctgcatat atgcaatccg aacaatcctg tatttgacaa tgccctgcca
      961 gaagaggtca acctccctgc cagtgtcatc cgtgatctgg gaaaccacaa gctgattgtc
     1021 ggacgcatga tggatatctg catcatcgat cttaacaaat attattcagg acagcccgat
     1081 tattattcca taataggcag gagccaggga ttcccgggca acgactgcca ggacaacgga
     1141 attatgcagg ataaggaagg ccgatggtgg attctgacct ctgacaagct gatctgtttt
     1201 gatccggata agatcagcag gaacacacat cctcccatga accacatcac caaagtcgag
     1261 atccagggca aagaagcgcc ctgggaaacg gctctggagg cctccctctt ctatgacaca
     1321 accagcttcc tgaagatcaa aggaaaacag aaccgtatca gaataagcta caccggtata
     1381 tcaacaagaa atccggaggg tgtcagatat cgctacagga tggtgggttt taatgacaac
     1441 tggtcagaaa agaccacaga gcggtcagtt gtttactcca acctgcctcc cggcgaatat
     1501 ctcttcaggg tccaggcaat aaacgctgac ggtgttctgt ctgaggaacc tgaccaactg
     1561 acaatcagca ttgtgccaac attcttccag tcaaaaatcg tcaatgcaat aatgatgatg
     1621 ctggccctct cgctgatggt attcctttcg tttcagatac gcaaggctgt actggtaaaa
     1681 agggttacca cggcacggag agaggctgaa gcatacaggc tgcagctaaa ctccgtgatc
     1741 aggcagtttg acccgcattt cacgttcaat gccgtaacct cagtaggatc actcattatg
     1801 aaaggtgaaa aggagaaagc ctataattat ttcattaaac tgtcaaacct tctccgttcc
     1861 atcatatctg acagcaacgt gcttctgagg cccctggagg atgagattga gttcgttaca
     1921 cgctactgcg agctccagaa actgcgcttt ggcaacaggt tcgaatacac ggtaaatatt
     1981 gacagtgatg tcaaccttaa aagccccgtg ccgaaaatga tcatacagtc attcgttgaa
     2041 aatgcaatca aacatggcct ggagaacaag aaagagcagg gtatcctcag gatcagcatc
     2101 agcaactgcg gggctggtat ggaagtgatt atactcgaca acggaatcgg cagggctgct
     2161 gccgctgatc tgcaaacaca cggaccaggt accggactga aaaatatagc aggcattgtt
     2221 gagaccctga acaaagtgaa caatgagaaa attaccttca ccctgactga cctgcatgac
     2281 aatggcaggg catcaggcac aaaggtaaca gtattcctcc cgtacaacta ctccctcgac
     2341 ctgactgctg atatgtagtg ataaatcaac ccaaacccta atagcatgaa ttcccggaaa
     2401 atcagatcaa tgatcgtcga ggacgaaccg gaggccctcg atctgctgac aggactgctg
     2461 gaagccacgg gagaggcaac ggtggttgcc tcaactacaa atccttttga ggccatcgat
     2521 ctgttgctgt tgcatacacc tgacatcctg ttcctcgaca ttaaaatgcc ggggatgtca
     2581 ggatttgaca taatcaatga gatgaacagg ctgaccgccc tgacaccaca cgttgttttc
     2641 acaaccgccc atgatgagta tgcaatcaaa gcgttcgact gtgctgcctt cgactacctc
     2701 ctgaaaccaa ttgaccctga gcggctggcc gagactctcc accggcacag cagccagacc
     2761 gtccggaaaa agagagagca gctgcagcaa caatcttttc cgaataatga gaagatactt
     2821 atcttccgcg gtgtcaccgg ggtgactttc attgataccg atgaggtggt ttatattacc
     2881 gctgacggaa actactcaag ctttcatttc atttccggaa gaatagaaac agtaacctcc
     2941 cttatcggaa acattgaggc gcagctggga aagcagtttt tcaggacaag caggtcatgc
     3001 atcatcaaca cgtcatatct tgcacgcatc gacatgaaac agttgtcctg cgtatttatc
     3061 aaaggcgaaa aggaattcag gtgcgagatc tcgcgtgaca aggtaaaaat gctcacggat
     3121 ttcatgaaaa accgtttctt cgaactctaa aatgaagtga ctttttttta catttttagc
     3181 tggtgcctgt ccgtacttta gaagctgcct ttcatacaat agaatacttt ttaaacagaa
     3241 agattgctat tttattcaat ctttaccacc gtatttcatt ttacggtaaa ctagggtttt
     3301 ttaacaaacg acaggtggtt cttccccccc ccatttccct gtcaggccac acagatcact
     3361 gcagaaaggt ctgtgtggcg cgtttttatg gggtagatga attaaatctc aatcctgaaa
     3421 acccacacgt aatcagacga gggtctgttc ctgacgtggt gtggtacaga aacggtaaat
     3481 gacccgttcc cgttcttcca cctgcatacg gctccggaac cgggcactga cacttttgcc
     3541 ccggccggca gagtgagtcc gttcatcgta atggcggccg gcatctgttc gccttcatca
     3601 gccatataat agaggtacca ggtgtcatcg cctttccggg tgatgcagct cttgccgtcc
     3661 ttatgcgggg caagcgccct cgttccgtaa atggcttcac cgttcacctt tatccatctc
     3721 cctatctcct caagcaggga gtaagcctcc gcgggccatg tgccgtcagg ggcaggacct
     3781 atgttcagca acaggttacc gccctttacg actatatcaa ccagcaggtg aacagcctcg
     3841 cgtggcgaca tgaacttcgg atcgggcacc catgcccagc cgccaccggc aatgatgcat
     3901 gactcccacg ggtatggcag cgccttctca ggcaccctgt tctcaggcgt caggtaattc
     3961 tggttctttc cgtacacagc cctgtcaaca acaatcagtc ccggctgcgc ctgccgcgct
     4021 ttggccacca gctcatccat acggatatcc tggctctgca ccctggtcat cttgttgccc
     4081 tgggccatcc tggacctgag cacctcatca tcggtcatct tcctgaccca gccgccatcg
     4141 agccacagga tgtcaatcgt gccataatcg ccgccaagga gttccataat ctgtccgtgt
     4201 gtgaactcca caaacttctc ccagcgctcc gggtaatcag tgatgctata gttgacatgg
     4261 cggtcaggcg tggcaaagtt tggccaccag tagtactcgc tgtgccagtc aggcttcgag
     4321 aagtaggcac ccgtccataa tccctgttcg cggaaagccc tgaatatctc ctttgttacg
     4381 ttagcgcggg gattaacgct gaacgggcac tccgggtcag ttatcctgta gtctgtcagt
     4441 tttgtgtcaa acattgaaaa accgtcatga tgcttggtag tgaaaaccac gtatttcatt
     4501 ccggcattcc gtgctgcagc agcccatttc tccggatcga aagcaatcgg attaaagctg
     4561 agcttcaggg cttcatactg ttttttatac tctgtgtaat cagggttttt gcgacggcac
     4621 cagccctcat cctcggcgca gatcgaccat gattcaacta tcccccactg actgtaaggt
     4681 ccccagtgca tcaggaggcc gaacttcagg tcctgccact cttcgagttt ccggagcacc
     4741 agcggatcac tctccggaat atatctttca tcgtgatcct gcgccacgag cggcccggca
     4801 aaaagggcgg caaccactac tgccggcaga caggcccgca tccatctcat gacagaacgg
     4861 aagatgcttt tcatgtcaac agatactaat cagtttcaag tttcccattg agcgagatgc
     4921 caaccacggt cattgtctta tccaatgtct cttcaggcac tctgatgaag tagaccccgg
     4981 cgtggtcgct ccaatatggt ctcaggtatt ccttcacctc gaggttggtg ccgttgccta
     5041 ccacgaaagc cttgttgata cggttcttca ccccttcgag catcagctca ccgccgccgt
     5101 taccgggaaa gaagagatag agtatggtgc tgtcgcggct catggtgg
//
LOCUS       DAOPBK010000169         5140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_13734_length_5140_cov_4.281613, whole genome shotgun sequence.
ACCESSION   DAOPBK010000169 DAOPBK010000000
VERSION     DAOPBK010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13734_length_5140_cov_4.281613"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLA17_09315"
     CDS             <1..238
                     /locus_tag="PLA17_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPK85651.1"
                     /translation="VFNIVRDCAAAVGITKTISPHTLRHSFATHLVEGGADLRAIQEM
                     LGHESITTTEIYTHIDRSYLRDTVLMYHPRARRR"
     gene            520..3249
                     /gene="ppdK"
                     /locus_tag="PLA17_09320"
     CDS             520..3249
                     /gene="ppdK"
                     /locus_tag="PLA17_09320"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPK85652.1"
                     /translation="MSKVKRVYSFGNKTAEGKADMRNLLGGKGANLAEMCLLGLPVPA
                     GFTITTDVCTEYYANNTQLPAELMPEVWEAMKKTEQAMGMKYGDSENALLVSCRSGAR
                     SSMPGMMDTVLNIGLCSKTIPGLIKKTSNPRFVWDSYRRLIMMYADVVMEKAEGIEPS
                     DGRGIRKQLDEMLDEYKHSKGYKADTDLTSDDLKNLAEEFKKRIKKTLGKEFPDDPRE
                     QLEGSIKAVFKSWNGKKAVSYRRIEGIPDEWGTAVNVQAMVYGNMGDSSATGVAFTRN
                     PATGENLFYGEWLVNAQGEDVVAGIRTPNPLNDDTKNEQNKHLHSMQELMPGTYRELV
                     EIRDILEKSFRDMLDIEFTIQEGTLYMLQCRSGKRTGTAALNMAMDMLQEGLIDEKTA
                     VMRVNPAQLDELLHPICDPDAEKKVSPLVKGLPAGPGAAVGKIVFTAEDAVAWNRRGE
                     KVILIREETNPEDVEGMRAAEGILTARGGMTSHAALVARGWGKCCIVGAGSLHVDVAG
                     KKVRITGSDVVLKEGDIITLNGTKGNVYTGSLKLMDASENPRFQSFMKLVDKNRTMGV
                     RTNCDNPVDAKMALEFGAEGIGLFRTEHMFYGLGAEKPLFILRKIILSESEEERRQAV
                     DELFPFVKKDMKGTLEAMDGRPVTFRLLDPPLHEFVPHSPETQAELAKALGITVEAIA
                     KRGESLHESNPMMGHRGVRLGITYPEISEMQIRAMFESTAELLKEGKNPVPELMVPVT
                     CDVSELDVTKKLYDKVLAEVLKKFNLSKIDHKFGTMIEIPRATLLADRMARTAEFFSF
                     GTNDLTQMTFGFSRDDIGGFLHEYLDSKMLDADPFQTIDQDGVGQLITMACEKGRATR
                     PDIKIGICGEQGGDPASVEFCFKNGLTYVSCSPFRVPIARLAAAQAAIKASRK"
     gene            complement(3448..>5140)
                     /locus_tag="PLA17_09325"
     CDS             complement(3448..>5140)
                     /locus_tag="PLA17_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPK85653.1"
                     /translation="TAPPYFFGPGTKDQHIPINGATVTLMPDNRTIITDDLNNGFFMF
                     DSLPPGNYEVIVSGMDDFYNDTLSASVIAGKTTLIDFLPSFDTARVPLVVRHLPATSD
                     SLSFNQSFIFTFNVPMDREAVQSALIFEPAADVRYEWDDKSKVLTVTPQTGFASKTTY
                     LIRLTTDATSLWDVHLQEEYQVSFVTKARPKLVIEKLWPAGNNTGITLYPRITVRFDA
                     PLDQASASAGIRLLDSQSAPLTKRSEVFTSDGGKGSYSFELTAPLQLNTSYHIEIDAS
                     VKDVAGVTLGEAVNATFTTRTKAYQTGHVVETFDNIGAFWDPEASGSTVGTDNPMTTF
                     TASNSVYKTNPPAGQLNYLFTGSDGGVCRVFNTSKPTIGSSTSLQFAMWVYGDLSNNY
                     LEYWFYSPGSVNQIVAAATINWAGWDLIAIPFSSIGGTGEWQYHSIVVVQNTNGLRSG
                     TMYFDDAMVISPTGIEEQANDDMELTVYPNPVRDEGRINFFLQSAGQVEIDLFSSDGH
                     LTANLFTGTMEAGAHTLPWNPSPAITPGSYTLRLTCRPAGKGVWRHESRRWVVLR"
BASE COUNT         1310 a         1307 c         1373 g         1150 t
ORIGIN      
        1 ggtgttcaac attgtcaggg attgtgctgc cgccgtcggg ataacaaaaa ccatcagtcc
       61 gcataccctc cgccactcct ttgccactca cctggttgag ggaggcgctg acctccgggc
      121 cattcaggag atgctggggc atgaatccat aacaaccacg gagatctaca cacatattga
      181 ccgcagctac ctgcgtgaca ctgtgctgat gtatcatccc agggcccgtc gccgctgatg
      241 ttgcaacagc cgcgaataca ttgttgcact gcaggaaaca gctcgcggca acagccgcaa
      301 atcattatca tccgacagcc ggaaacagat cactgcaaca gttgggagcc attggcccac
      361 tgaagaaaac agatctctgc aactgctgcc aatgcactgt ctcactgccg gtaaaggcga
      421 cccctgtaaa atgataaaag ctattccttt attcgtttct ttttattacc tttgcggatc
      481 gcaaaaaaag agagtattaa ttaataacca atatcgaata tgtcaaaagt taagcgcgtt
      541 tattcctttg gaaacaagac agccgagggt aaggctgaca tgagaaacct gcttggtggc
      601 aagggagcca acctggctga gatgtgcctt ctgggccttc cggtacctgc cggcttcaca
      661 atcaccactg atgtgtgtac tgagtattat gcaaacaaca ctcagcttcc ggcagaactg
      721 atgccggaag tatgggaagc catgaaaaag accgagcagg caatgggtat gaaatacggt
      781 gacagtgaga atgcactcct tgtttcatgc cgttcaggtg cccgcagctc gatgcccggc
      841 atgatggaca ctgttctcaa cattggcctc tgctccaaga ccatccccgg ccttatcaaa
      901 aagaccagca acccccgttt cgtatgggat tcataccgcc gtctgatcat gatgtatgca
      961 gacgtggtta tggagaaggc tgaaggaatt gagccttcag acggcagggg aatccgcaag
     1021 caactcgacg agatgcttga cgaatacaag cacagcaaag gttacaaagc agacaccgat
     1081 cttacatccg atgatctgaa aaaccttgcc gaagaattca agaagcgtat taagaagaca
     1141 ctcgggaaag agttcccgga tgacccgcgt gagcagctgg aaggcagcat caaagctgtt
     1201 ttcaagagct ggaatggcaa gaaggctgtt tcataccgca ggattgaagg aattcctgat
     1261 gagtggggaa cagcggttaa cgtgcaggct atggtgtacg gaaacatggg tgactcatca
     1321 gccaccggcg ttgcattcac ccgtaacccg gcaaccggcg agaatctctt ttacggagag
     1381 tggcttgtaa atgcacaggg cgaagacgtg gttgcaggta tccgcacccc gaacccgctt
     1441 aacgatgaca ccaagaacga acagaacaag cacctgcaca gcatgcagga gttaatgccc
     1501 ggaacataca gggaactggt ggaaatcaga gacatccttg agaaatcatt ccgtgacatg
     1561 cttgatattg agtttaccat ccaggaaggc accctctata tgcttcagtg ccgttccggc
     1621 aagcgtaccg gtaccgctgc cctgaacatg gcaatggata tgcttcagga aggtctcatt
     1681 gatgagaaaa ctgctgtgat gagggttaat cctgctcagc ttgatgaact cctccacccg
     1741 atttgtgacc cggatgctga gaagaaggtt tccccgctgg tcaagggtct gcccgccggt
     1801 cctggtgctg cagtaggcaa gatagtcttc acggcagagg atgcagtggc atggaaccgc
     1861 aggggcgaga aggttattct tatccgcgag gagaccaacc ccgaggatgt tgaagggatg
     1921 cgcgcagcag aaggcattct cactgcacgc ggtggtatga cctcgcacgc tgccctcgtg
     1981 gcacgtggct ggggcaagtg ctgcatagta ggtgcaggca gcctccatgt tgacgttgca
     2041 ggtaaaaaag tcaggataac cggatcagat gtagttctga aagagggtga catcatcacc
     2101 ctgaacggaa cgaaaggcaa tgtttacaca ggttcgctga aactgatgga tgcatcagag
     2161 aatcccaggt tccagtcgtt catgaaactg gttgacaaga accgcaccat gggtgtccgc
     2221 accaactgcg acaatcctgt tgatgcaaag atggcactcg aattcggagc tgagggaatc
     2281 ggtctcttcc gtaccgagca tatgttctac ggccttggtg cggaaaagcc gctctttatc
     2341 ctccgcaaga taatcctcag cgagagtgaa gaagagcgca ggcaggctgt tgacgagctc
     2401 ttccccttcg tgaagaaaga catgaaaggg acactggaag cgatggacgg acggcccgtg
     2461 acattccgtc tgctcgatcc gcctcttcat gagttcgttc ctcatagccc cgagacgcag
     2521 gctgagcttg caaaagcact gggcattacc gttgaagcca ttgcaaagag aggcgaatct
     2581 ctgcatgaat cgaacccgat gatgggccac cgtggcgtcc gcctcggcat tacctatccc
     2641 gagatctcag agatgcagat cagggctatg ttcgaatcta cggcagaact gctgaaagaa
     2701 ggcaagaacc cggtacccga gctgatggtc ccggtcacct gtgacgtttc tgaactggat
     2761 gttacgaaga agctgtacga caaggtcctt gccgaagtac tgaagaaatt caacctcagc
     2821 aagattgacc acaagttcgg aacaatgatt gagataccgc gcgccacgct gctggccgac
     2881 cgcatggcaa gaaccgccga gttcttctcg ttcggcacca atgacctgac gcagatgacc
     2941 ttcggattca gccgcgatga cataggaggc ttcctccatg agtatcttga cagcaagatg
     3001 ctggatgctg acccgttcca gaccattgat caggatggtg taggacagct gatcaccatg
     3061 gcatgcgaga agggccgtgc aacccgtccg gatatcaaga taggtatctg cggagagcag
     3121 ggtggtgacc cggcatcagt tgagttctgc ttcaaaaacg ggctcaccta tgtaagctgc
     3181 tcacccttca gggtacccat cgccaggctg gcagcagctc aggcagctat caaggcttca
     3241 agaaagtaag ctttattcca taacagggaa taagggtgtc tcaaaagggc accctttctt
     3301 tttgtaagga ggcatatctg tagtctcctc tttttaaata catctggcag tcggtcctgc
     3361 ccccgggaat cgttgccggg gatccccgat ccgctctgca agagagcacg acctcacgac
     3421 agaagatatt ttcagacctt cagagttcta tctcagcacc acccacctcc gtgactcatg
     3481 tctccatact ccttttccgg ctggccgaca ggtcagccga agggtatatg aacccggggt
     3541 gatggcaggt gacggattcc agggcagcgt atgtgctcca gcctccatgg taccggtgaa
     3601 caggttcgcc gtcaggtggc catcggagga gaagaggtca atctccacct ggccggccga
     3661 ctgcagaaaa aagttgattc tgccctcatc ccttaccggg ttgggataaa ctgtcagctc
     3721 catatcatca tttgcctgtt cttctatacc ggtaggcgag ataaccatag catcatcaaa
     3781 atacatggtg cctgatcgca ggccatttgt attctgcacc actacaatac tgtgatactg
     3841 ccactcgcca gtgccgccta tgctgctgaa gggtatggct atcagatccc atccggccca
     3901 gttaattgtt gcagcggcaa caatctggtt gaccgaccct ggtgagtaga accagtattc
     3961 aaggtagttg ttgctgagat caccgtaaac ccacatggcg aactgaaggc tggtactact
     4021 gcctatggtt ggtttgctgg tgttaaaaac acgacacact ccgccgtcgc tgccggtaaa
     4081 gagataatta agctgcccgg ccggcgggtt tgtcttgtaa actgagtttg aagctgtgaa
     4141 ggtggtcatg ggattatcag taccgacggt gctgccgctg gcttccggat cccagaaagc
     4201 gccgatatta tcaaatgtct ccaccacgtg accggtctga taagccttgg tacgggtagt
     4261 aaaggttgca ttcacagcct cgcccaatgt aactccagcc acgtccttta ccgatgcatc
     4321 aatctcaata tggtagctgg tgttaagctg caggggtgca gtcagttcaa agctgtagct
     4381 gcccttgccg ccatcggagg taaaaacctc actccgtttg gtaaggggag ccgactggct
     4441 gtcaaggagc ctgatgcccg cagatgcaga tgcctggtca agaggtgcat caaaccgtac
     4501 ggtgatcctt ggatagaggg tgatgccagt attattccct gcaggccaca acttttcaat
     4561 taccagtttc ggccgcgctt tggtgacaaa gctgacctga tactcctcct gaagatggac
     4621 atcccataag gaggttgcat cagtggtcag cctgatgaga taggtcgttt tggaggcaaa
     4681 gcctgtttga ggagtgaccg taagcacctt gcttttatca tcccactcat atctcacatc
     4741 tgctgccggc tcaaatatga gtgctgactg gacggcctcc cggtccatgg ggacgttgaa
     4801 tgtgaagata aatgactggt tgaatgacag actgtcactg gttgccggca gatgccggac
     4861 taccaacggc acccgtgcag tgtcaaaaga ggggagaaag tcaatcaggg ttgtcttgcc
     4921 tgcaattacc gaagctgaaa gagtatcatt gtagaaatca tccatcccgc tgacaatgac
     4981 ctcgtagtta ccgggaggca gtgagtcaaa cataaagaat ccgttgttca ggtcatcagt
     5041 aatgattgtc ctgttatccg gcatgagggt aactgttgct ccgtttatcg gaatgtgctg
     5101 atcttttgtg cccggaccaa agaagtatgg cggagcggtg
//
LOCUS       DAOPBK010000170         4919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_14419_length_4919_cov_4.700658, whole genome shotgun sequence.
ACCESSION   DAOPBK010000170 DAOPBK010000000
VERSION     DAOPBK010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14419_length_4919_cov_4.700658"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLA17_09330"
     CDS             <1..796
                     /locus_tag="PLA17_09330"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772359.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HPK85654.1"
                     /translation="IDATIALGGQGYVFWGGREGYLTLHNTDMKRELDHLAMMLTMAR
                     DYGRKNGFKGLFYIEPKPMEPTKHQYDYDAATVIGFLRHYGLDKDFALNVEVNHATLA
                     GHSFQHDLQTAADAGMLGSIDANRGDYQNGWDTDEFPTNINEITEAMLVIMMAGGFKT
                     GGINFDAHVRRNSTDPEDLFIAHINGMDTFARGLVIADSILKDSDYLKMRKARYASFD
                     QGNGALYEKGKLTLEDLRELAKKVGEPKKISGKQELYEQLINMYII"
     gene            804..2276
                     /locus_tag="PLA17_09335"
     CDS             804..2276
                     /locus_tag="PLA17_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPK85655.1"
                     /translation="MAVFTPLRRWQPAFRPFSRQSAGKRLLALTERIIKGPLFGCRMC
                     GNCLLQETAFICHMACPKGIRNGPCGGVNNGYCYVDPSRRCAWNLIYERSRGMHREHK
                     LLEVLPPVDWEKAGTETWGEVVRTARKTGIRNIFVPSRRKGQTMGSVLEESIFKPIRQ
                     PDWWKGDSEYHPPAVSGPQSSLETELRSGRFVITAEVTPPLTASTVKLREKILMVMPY
                     VSAVNFTDGSSAVPRMSGAACSAVAASLGADPVMQISARDNNRNSLQSMAIGANAQGV
                     YNILCLSGDSPRVGRAPWSSMNIIDVDSVQMLWILRRMRDEGIYLDGREIKNRPKLFL
                     GAAAAPFALDPAMQAIRIRKKVNAGAQFIQTNLVFDPDRLDPWLEQLEKENLLDKVFI
                     LVGISPIKSLSVARYLNENIPGVTVPERVMQRLADAGDRFTGESIDITVETLLKLKSK
                     KGISGVHIMPLGWEDAIPLIIKKAAHETTDFKGAGQGRQS"
     gene            2230..4026
                     /locus_tag="PLA17_09340"
     CDS             2230..4026
                     /locus_tag="PLA17_09340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HPK85656.1"
                     /translation="MKLLTSKELARAANLDIPGGVVIAKALMQIFRYNKLNRVYTNTY
                     DSDPAIFINSILDHLDIRYDIPENDLENIPLTGPFITVSNHPFGGIDALILLKILARR
                     RSDVRVMANFLLQKIDPLKHLIVPADAEGERYQGKVSLKGMEEAMNHVREGHGLALFP
                     AGEVSAYHSESGLITDMEWQEQILREIKMAEVPVIPVYFHGTNSRWYHILGRIHPLLS
                     TAKLGSELFNKRHKVIRVRIGRSLSVSEQAGFGDIARFGRYLRARTYSLGSSLEAKPF
                     FAGLARRRNKRQEPVAPPSDRATLAAEISNIRRTHELFAIGNFVVLFAPTRAIPNLIR
                     EIGRMREVTFRAVGEGTNRATDLDEYDFYYHHLVIWDEAAGVLVGAYRVGKGREIISQ
                     YGIKGFYINSLFRIRKSFEPVLAESLELGRSFIVQEYQKKAMPLFLLWKGLLTVLLQN
                     AGYRFLIGPVSISNEFSAFSKSLIVEFVRSNYFDREMARYIRPRKKFTPQIDRRIDSE
                     IIISSAERDINKVEKVVSDVDKGYRIPVLLKKYLEVNARIIGFNVDPDFNNCLDGLIM
                     LDIHNLPAGFVSSLSKDQNEAEVARRLRRRDS"
     gene            complement(4121..>4919)
                     /locus_tag="PLA17_09345"
     CDS             complement(4121..>4919)
                     /locus_tag="PLA17_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPK85657.1"
                     /translation="DQSRRVRKYDIGIVDRQIIPGSMTTQRIYSDASYFAGNNETYEK
                     FNKAVAEGNLNKKLALNVTPDQKELPGLTQSRELVMALFQNSKAGSIITDNSNQAVFE
                     LPDQYVVAYCSRAQEEGVAPVEDVASEIRFILAKKKKAEIIAAEMKKLEAEGKSLNEI
                     AAHYNTTVQDASGINFRSYSIPGAGIEPALISAASASEAGKLSKPVEGNNGVYMFMVS
                     EVTPVAAEDLEMVKERMNSSYQIRASYEAYQALRDKNEVKDMRYKFY"
BASE COUNT         1158 a         1271 c         1340 g         1150 t
ORIGIN      
        1 cattgatgca acaattgctc tgggcggaca gggttatgtt ttctggggcg ggcgcgaggg
       61 ttacctcacc cttcacaaca cagatatgaa gcgtgagctt gatcacctgg caatgatgct
      121 taccatggcc cgtgactatg ggcgcaagaa cggcttcaag ggtctgttct atattgagcc
      181 caagcccatg gagcccacca agcaccagta tgactatgat gcagccacgg taattggttt
      241 cctgaggcac tacggccttg acaaggactt cgcccttaac gtggaggtca atcatgccac
      301 ccttgccggc cactctttcc agcatgacct gcagactgca gccgatgccg gcatgctcgg
      361 cagcattgac gccaacaggg gagactacca gaacgggtgg gataccgacg agttcccgac
      421 aaacataaac gagatcaccg aagcgatgct ggtaatcatg atggccggag gcttcaaaac
      481 cggcggcatc aatttcgacg cacatgtacg ccgcaactcc accgatccgg aggacctctt
      541 cattgcccat atcaatggca tggatacctt tgcccgtgga ctggtgattg ctgacagcat
      601 cctgaaggat tcagattacc tgaaaatgcg caaagccagg tacgcttcgt ttgatcaggg
      661 taacggcgct ctctatgaga agggcaagct gaccctcgag gaccttcgtg agctggccaa
      721 aaaggttggt gagccgaaaa agatcagcgg caagcaggag ctttatgagc aactgataaa
      781 catgtatatt atctgacaga tcaatggctg tcttcacgcc cctccggcgc tggcagcctg
      841 cattccgtcc tttcagcagg cagtctgccg gaaaaagatt gcttgcactg acagagagga
      901 tcatcaaggg tccgctcttc ggatgcagga tgtgcggcaa ctgcctgctg caggagactg
      961 catttatatg tcacatggcg tgtcccaagg ggatacgcaa cggcccgtgc ggcggggtga
     1021 acaatggcta ctgctatgtt gacccctcac gccgctgtgc atggaacctg atttatgagc
     1081 ggtcacgggg catgcaccgt gaacacaagc ttctggaggt actgccaccc gtagactggg
     1141 agaaggccgg aactgagaca tggggcgaag tggtacgcac ggcacggaaa acaggcatca
     1201 ggaatatatt tgtcccctcc cggaggaaag gccagaccat gggcagcgta ctggaggaaa
     1261 gtattttcaa acccatccgc cagcccgact ggtggaaagg tgactcagag taccatccac
     1321 ctgccgtttc cggacctcag tcatcactcg agacagagct gcgcagcggc aggtttgtca
     1381 tcactgccga ggtgacacct ccgcttactg cctccacggt gaagctgcgt gaaaagatac
     1441 tgatggtcat gccttatgtc tcggcagtca attttactga cggatcatca gcggtaccga
     1501 ggatgtcagg cgctgcctgc tctgccgtgg cggcctcact cggtgccgac ccggttatgc
     1561 agatctcggc gcgtgacaac aaccgcaaca gcctgcagtc tatggccata ggcgccaatg
     1621 cccagggtgt gtacaacata ctgtgcctct cgggcgacag ccccagggtg ggccgcgctc
     1681 catggagcag catgaacatc atcgacgtcg actcggtgca gatgctatgg atactgagaa
     1741 ggatgcgtga tgagggcatt taccttgatg gaagggagat aaaaaacaga ccgaagctct
     1801 tccttggcgc tgctgctgcc ccgtttgcac ttgatccggc aatgcaggcc atccgtatac
     1861 gcaaaaaagt caatgccggg gcccagttca ttcagacaaa cctggtcttt gatcctgacc
     1921 gcctcgatcc atggctggaa cagcttgaaa aggagaacct tcttgacaag gttttcattc
     1981 ttgtgggtat cagcccaatc aaatcactct ccgttgcccg atatctgaat gagaatatac
     2041 cgggagtgac cgtgccggag cgggtgatgc agagactggc agatgccggt gaccgtttca
     2101 ccggggagag cattgacatc accgttgaga cactgttgaa gcttaagtca aaaaagggaa
     2161 tcagtggagt acatataatg ccactgggct gggaagatgc catccctctg ataataaaga
     2221 aagcagcaca tgaaactact gacttcaaag gagctggcca gggccgccaa tcttgacatt
     2281 ccgggcgggg tggtgattgc caaagccctg atgcagatct tccgttacaa caagcttaac
     2341 agggtctaca caaataccta tgacagtgac cccgcaattt ttatcaattc catccttgac
     2401 caccttgaca tcaggtatga catacctgaa aatgacctcg agaatattcc gctgacgggc
     2461 cccttcatta cggtctcgaa ccatcccttc ggcgggattg acgctcttat acttctgaag
     2521 atacttgccc gccggcgttc tgatgtcagg gtgatggcca acttcctgct gcagaagatt
     2581 gatccgctta agcatcttat cgttcctgcc gatgcagaag gagagaggta tcagggaaag
     2641 gtatccctca aagggatgga agaggcgatg aatcatgtca gggagggcca tggcctggct
     2701 cttttccccg caggggaggt ctcagcctac cattcggaat caggactgat aactgacatg
     2761 gagtggcagg agcagatact gcgcgagata aagatggctg aggtgccggt cattccggtc
     2821 tattttcacg gcaccaactc aaggtggtat catatactgg ggcgcattca cccgttgctg
     2881 agtactgcaa agctgggctc ggaactgttt aacaagcgtc acaaggtcat cagggtacgc
     2941 atcggacggt cactgtcagt cagtgagcag gccggattcg gagacattgc ccgtttcgga
     3001 cgttacctca gggcccgcac ctattcactc ggctcatccc tggaggctaa acctttcttc
     3061 gccggtctgg cacgacggcg caacaagcgg caggaaccgg ttgcccctcc gtctgacaga
     3121 gcaacgcttg ccgctgagat tagtaacata agacggacac atgaactgtt tgccattggt
     3181 aacttcgttg tcctttttgc ccctacacgg gctatcccga accttatcag ggagatcggc
     3241 aggatgcgcg aggtgacctt cagggcagtc ggtgaaggga ccaacagggc gaccgacctg
     3301 gatgagtatg atttctacta tcaccacctg gttatctggg atgaggcagc cggtgtactc
     3361 gtcggggctt accgcgttgg aaagggaaga gagattatca gccagtatgg tatcaagggt
     3421 ttttatatca actccctgtt caggatacgc aagtcatttg aacctgtcct ggctgaatct
     3481 cttgagttgg gacgctcatt catcgtgcag gagtaccaga aaaaggcgat gccactgttc
     3541 cttctctgga agggtcttct gacagtgctg ctgcagaatg ccggataccg gtttcttata
     3601 ggcccggtga gcatcagcaa tgagttctcg gcgttttcca aatccctgat agttgaattt
     3661 gtcaggagca actactttga ccgggagatg gccaggtaca tcaggccgag gaagaagttc
     3721 acgccccaga tagacaggcg tattgacagt gagatcatca tctcatcagc ggaaagggat
     3781 atcaataagg tggaaaaggt ggtcagtgat gtggacaagg gttaccggat accggtgctg
     3841 ctgaagaaat accttgaggt caatgcccgg atcatcggct tcaatgttga ccctgatttc
     3901 aacaattgtc ttgacgggct gatcatgctg gacattcaca atctccctgc cggatttgtc
     3961 agttccctgt cgaaggacca gaacgaggca gaggtggcac ggcggctgag acgcagggat
     4021 agttaggtat ttccctctta catccttaat actatggtat gtaaaatcgt agcggcagat
     4081 ctcggatctg ccgctaccat atatgtttat ttaccggata tcaatagaat ttgtacctca
     4141 tatccttgac ctcgttcttg tcgcgcaggg cctggtaggc ttcgtatgaa gcacggatct
     4201 gatagctgga gttcattctc tccttcacca tttccaggtc ttcggcagcc acgggagtga
     4261 cctcactgac catgaacatg tagacaccgt tgttgccttc aaccggtttt gaaagtttac
     4321 cggcctcaga ggctgatgct gcggatatca gggcgggctc tatcccggcg ccggggattg
     4381 agtatgacct gaagttgatg ccgctggcat cctgaacggt tgtgttgtaa tgagcggcaa
     4441 tctcattgag gctcttgcct tcggcttcaa gctttttcat ttctgcagca atgatctccg
     4501 cctttttctt ctttgccagg atgaacctga tttctgaagc tacatcctcc acgggtgcaa
     4561 ctccttcttc ctgggccctg ctgcagtagg caaccacata ctggtcagga agctcgaaca
     4621 ctgcctgatt gctgttgtca gtgataatgc tgcccgcctt gctgttctgg aagagggcca
     4681 tgacaagttc tcttgactgt gtcaggccgg gaagctcctt ctggtcaggt gtaacgttca
     4741 gggcaagttt cttgttgaga ttgccctcag ccacagcctt gttgaacttc tcgtatgtct
     4801 cattgttccc ggcaaaatat gaagcatcag aatagatgcg ctgcgttgtc atgctgccgg
     4861 ggatgatctg acggtcaacg atgcctatgt catacttcct tacccggcgg ctctggtca
//
LOCUS       DAOPBK010000171         4906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_14459_length_4906_cov_4.608740, whole genome shotgun sequence.
ACCESSION   DAOPBK010000171 DAOPBK010000000
VERSION     DAOPBK010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14459_length_4906_cov_4.608740"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="PLA17_09350"
     CDS             <1..1634
                     /locus_tag="PLA17_09350"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPK85658.1"
                     /translation="IRDYFCHNLYGKMGNYASRAVYCEVIINNNYAGLYMLQEKLKAD
                     DNRINVTKIGKNDNALPALSGGYISKADKTTGGDPVAWTMYSFQNSPVEYIHDLPKPE
                     NATTEQTSYISNLFFRLESAVQNNDVSVVNGYPSLIDIPSFIDFMIVNEFASNPDAYQ
                     YSTYFHKDRNGKLRAGPVWDLDLTFGNDLFHWNMDRSKTNLWYFEDHFYNNGSRFWRD
                     LFHNSVFRCYLSKRWNELLEPEMPLNRPVREAFIDETVELISEAIARDCQRWNKQTNH
                     TERIAAIKSFMQAREEWITAGLGGYSTCSSVEVPQLTITKIMYHPQTSAQFPDADDLE
                     FLEIRNTGTAIVNLSGIYFMGTGFVYQFPANTTLGPGRCVILASNATIFQAKYGFSPF
                     GQFTRHLSNSGESLILADAFGNVIDVTEYSNEAPWPDAGGNGSYLSLKDTALDNSLPE
                     SWEALSEVITSAAEPMADIAVSLYPNPVGDLLTVTAETEISTICVYSMGGTILLMQDA
                     GSRKTTVNMSGMLPGVYIIRIFTREGSFPRMVVKK"
     gene            complement(1639..3057)
                     /locus_tag="PLA17_09355"
     CDS             complement(1639..3057)
                     /locus_tag="PLA17_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HPK85659.1"
                     /translation="MKRLIVLLFPILFVSCNRPPENGPGKNGGTGIMAEGWITSPQKG
                     ILFTKHPGELEFMTSANTDPTIVVDTTQRYQEIDGFGNCLTGGSAILLNNMSQPERTR
                     LLRELFSSDGNGIGISYLRITIGASDLSDRVFTYCDLPPGETDPQMEHFSIEPERADL
                     IPVLKEILAINPSLKILGSPWTAPLWMKTNNASVGGSLKHEWFEAYANYFVKYIQAMK
                     AEGIDIDAITVQNEPLHGGNNPSMVMSAADQALFIRQNLGPAFATAGIETKIIIYDHN
                     ADRTDYPLAVLNDAGARQYIDGSAFHLYGGSIDALTAVHDAFPEKNLYFTEQWVGGPG
                     NLAEDLKWHVENLIVGATRNWCRNVIEWNLASDPSYDPHTEGGCDRCLGTVTLSGNNV
                     TRNPAYYILAHAARHVRPGSVRVSTNALGNLPNVAFQTPSGSKVLIVVNTTPDKRSFN
                     VKFRGNTLPLSLDGGAVGTWIW"
     gene            complement(3054..3893)
                     /locus_tag="PLA17_09360"
     CDS             complement(3054..3893)
                     /locus_tag="PLA17_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HPK85660.1"
                     /translation="MIQAGILLLAFALLAPGCGRPEKPKEYPDNPNTGDMVLVWSDEF
                     DYNGLPDSKKWSYDTEGNATGWGNNESQYYTASRLKNAEVKDGFLSITAHREDFEGFK
                     YTSARLVTRQKGDWLYGRVEVKAKLPDGRGMWPAIWMLPTDWSYGGWPASGEIDIMEN
                     VGYDPYKIVGTVHTEAYNHMIGTQKGATTNVQSCYGDFHIYAIEWEVREIRFYVDDLH
                     YFTFTNEGTGYKAWPFDKRFHLLLNVAVGGNWGGMQGIDDSIFPRSMIVDYVRVYQKK
                     TGK"
     gene            complement(3911..>4906)
                     /locus_tag="PLA17_09365"
     CDS             complement(3911..>4906)
                     /locus_tag="PLA17_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPK85661.1"
                     /translation="GTWCFDGREENSVTPLKAIRDLLGDKKVNYVQALAYSRDKSKAY
                     FAEAVSAARRSDAVLFFGGEEWILSGEGQSRGEINMPGVQTGLLEALAATGKPVILVI
                     MAGRPLAIGRELELSDAVLYAWHAGTMAGPAIADLIFGKESPSGKLPVTFVKGAGQIP
                     FYYYRNNTGRPATDEAWTPFDSIPTVNPQSSLGYRTFHLDYGYTPLLPFGYGLSYTTF
                     KYSDLTLSSQVMKIDGTLTVRARVTNTGNMDADEIVQFYVRDRVGSITRPIKELKGYN
                     RLRIKSGETADVEFTLTASDLAFFNGRETVTEPGKFDVWIGPSSDEGLHADFILE"
BASE COUNT         1186 a         1322 c         1183 g         1215 t
ORIGIN      
        1 tcatacgcga ctacttctgc cataaccttt acgggaagat gggcaactat gcctccaggg
       61 cagtctattg cgaggtaatc ataaacaata actacgcggg gctgtacatg ctccaggaga
      121 agctcaaggc tgacgataac aggattaacg tgacaaaaat cggcaagaac gacaatgctc
      181 ttcccgcgct atccggcgga tacatatcaa aggctgacaa aacaacaggt ggtgatccgg
      241 ttgcctggac gatgtattca ttccagaatt ccccggtaga atatatccat gacctgccca
      301 aacctgaaaa cgccaccact gaacagacat catatatcag caatctcttc ttcaggcttg
      361 aatcagccgt gcaaaacaat gatgtctccg tggtgaacgg atatccctca ctgatagaca
      421 ttccttcttt cattgatttc atgattgtga atgaatttgc ttccaatcct gatgcctacc
      481 agtacagcac atatttccac aaggaccgca acgggaagct tcgcgcagga ccggtctggg
      541 accttgacct cacctttggc aatgacctct tccactggaa tatggaccgg agcaaaacca
      601 acctgtggta cttcgaggac catttttaca acaatggttc ccgcttctgg cgggatctgt
      661 tccataacag cgtgttcagg tgttacctct caaaaaggtg gaatgaactg ctggagccgg
      721 agatgccatt gaacaggccg gtaagggagg cttttattga cgagaccgtg gaactgatat
      781 ctgaagccat tgcacgagac tgccagagat ggaacaagca gacaaaccat acggaaagaa
      841 ttgcagccat caagtcattc atgcaggccc gtgaagaatg gataaccgcc gggctcggag
      901 gctacagcac atgcagcagc gttgaagtcc cgcaactgac gataacgaag ataatgtatc
      961 atcctcagac ctcggcacag tttcctgacg cagatgatct ggaattcctg gagatacgaa
     1021 ataccgggac agccatcgtg aaccttagcg gtatatactt catgggtacc ggatttgttt
     1081 atcagttccc tgccaatacc accctcggac ctggccggtg cgtgattctg gcatcaaatg
     1141 ccaccatctt ccaggctaag tacggtttct ctcccttcgg gcagttcacc agacatctct
     1201 caaacagcgg tgagtccctc attctcgctg atgccttcgg gaacgtgata gatgttacag
     1261 agtattcaaa cgaggctcca tggcctgatg caggcggcaa cggttcatat ctcagcctga
     1321 aagacactgc gcttgacaac agcctgccgg aaagttggga ggctttatct gaagtgatca
     1381 cctctgccgc tgaacccatg gcagacatcg ctgtaagcct ctatcccaac ccggttggtg
     1441 atctgctcac tgtcacggca gaaaccgaaa taagcactat ctgcgtttac agcatgggag
     1501 gaaccatcct gctgatgcag gatgccggca gccggaagac cacggtcaac atgagcggca
     1561 tgttacccgg ggtctacatc ataaggattt tcacccgcga gggcagcttt ccacgcatgg
     1621 tggtaaagaa atgacagcct accaaatcca tgtaccaact gctccgccgt ctaggctgag
     1681 cggaagggtg tttcccctga actttacgtt aaagcttctt ttgtcgggtg tggtgtttac
     1741 tacaataagt accttgcttc cggacggggt ctgaaaggca acattgggca ggtttcccag
     1801 tgcattggtt gagactctca ccgatcccgg ccttacatgc ctggcagcgt gggcaaggat
     1861 ataatatgcc ggattcctgg tgacgttgtt accgctgaga gtcacagttc ccaggcacct
     1921 gtcacaacct ccttcagtat gcgggtcata cgaggggtca gatgccaggt tccattcgat
     1981 cacgtttctg caccaattac gggtagcgcc aactataagg ttttcaacat gccatttgag
     2041 gtcttcggcc agattgcccg gtccgcctac ccattgctcg gtaaagtaaa ggttcttttc
     2101 cgggaaagca tcatgaaccg cggtgagggc atcaatcgat cctccgtaaa gatgaaacgc
     2161 cgagccgtca atatattgcc gggctccggc atcattcaac acggcaagag gatagtctgt
     2221 ccggtcagca ttatggtcat agataatgat tttggtctct atccctgccg tggcaaatgc
     2281 cggccccagg ttttgcctga tgaaaagagc ctgatctgca gccgacatta ccatacttgg
     2341 attgttgcca ccatggaggg gctcattctg gacagtaatg gcatcaatgt caataccctc
     2401 tgccttcatg gcctggatat acttcacaaa atagttcgcg taggcttcaa accattcatg
     2461 tttcaggctt cctcccacag atgcattgtt ggtcttcatc cataacgggg cagtccaggg
     2521 tgagcccaga atcttaagtg acggattgat ggccagaatc tccttcagta caggaatcag
     2581 gtcagctctt tccggctcaa tgctgaaatg ctccatctgc gggtctgttt cacccggagg
     2641 caggtcacag taggtaaaga ccctgtcact gaggtcagag gcgccaatgg ttattctcag
     2701 gtaactgata cctatgccgt ttccatcact gctgaaaagc tcgcgcaaca gcctggtgcg
     2761 ttcgggctga ctcatgttat tgagaaggat ggcgctgcct ccggtgaggc agttcccaaa
     2821 gccgtctatc tcctgataac gctgtgttgt gtcaacaacg atggtcgggt cagtattggc
     2881 actggtcatg aactcaagct ccccgggatg cttcgtaaac aggataccct tctgcggcga
     2941 agtgatccat ccctcagcca taattccggt tccgccattc ttcccgggac cgttttccgg
     3001 aggcctgttg cagctgacaa acaggatggg gaaaagcagc acaatgagcc ttttcatttt
     3061 cctgttttct tttggtagac ccttacgtaa tcaacaatca ttgaccgtgg aaagattgaa
     3121 tcatcgatac cctgcatgcc tccccagttg ccacccactg ccacattgag caacagatgg
     3181 aaccgtttgt caaaaggcca ggccttgtag ccggtgcctt catttgtaaa agtaaaataa
     3241 tgcaggtcat ctacatagaa cctgatctcc ctgacctccc attcaatggc atatatatgg
     3301 aagtcaccgt agcacgactg aacattggtg gttgctccct tctgtgtgcc gatcatgtga
     3361 ttatatgcct cagtgtgaac cgtgccaacg atcttgtaag gatcataacc aacattctcc
     3421 attatatcta tttcgccact ggcgggccag ccaccataag accagtcggt aggcagcatc
     3481 catattgccg gccacatccc tcttccgtca ggaagttttg cctttacctc caccctgcca
     3541 taaagccagt cacccttctg acgtgtgaca agcctggccg aggtgtattt aaaaccttca
     3601 aaatcttcac gatgtgccgt tatggagaga aaaccgtcct tcacctcggc gttcttcagc
     3661 cttgatgccg tataatactg agactcattg ttaccccagc ctgtggcatt gccctctgta
     3721 tcatagctcc actttttcga atccggcaac ccgttataat cgaactcatc gctccacaca
     3781 agaaccatgt ctcccgtgtt tggattatcg ggatactctt tcggtttttc cgggcggcca
     3841 catcctggcg ccaggagggc gaatgccagc aaaagtattc cggcttgtat catctttgcc
     3901 atttttttat ctattcaagt ataaagtcag catggagccc ttcatcagaa cttggcccga
     3961 tccagacatc gaactttccg ggttcggtta ccgtctccct gccgttgaaa aaggcaaggt
     4021 cagaagccgt cagggtaaac tccacatcag cagtctcgcc acttttgatg cggagacgat
     4081 tgtatccctt cagttccttg atcgggcgtg ttatgctgcc gactctgtcg cgaacgtaga
     4141 actgcactat ctcgtcagca tccatgttgc cggtattggt caccctggcc ctgacggtaa
     4201 gtgttccgtc aatcttcatc acctgtgaag agagggtgag atcactgtat ttaaaagtcg
     4261 tgtagctcag gccataacca aatggcagca aaggagtata gccgtagtca aggtggaacg
     4321 tacggtatcc gagtgagctc tgcgggttga ccgtggggat ggagtcgaac ggtgtccatg
     4381 cctcatcggt tgcaggcctt cctgtattgt tcctgtaata atagaaagga atctgtcctg
     4441 cccccttgac aaaggtgacc ggcagtttgc ctgaaggaga ctccttgcca aagatcaggt
     4501 cagctatagc cgggccggcc atggtgcctg catgccaggc ataaagcacg gcatcggata
     4561 gttcaagttc cctgccaata gccaggggtc gtccggccat gattaccagt atgacaggct
     4621 tgcctgttgc cgccagcgct tcaagcagcc cggtctgcac tccgggcata tttatctctc
     4681 ccctcgactg accttcacct gagaggatcc actcctcgcc accgaagaag agaaccgcat
     4741 cagacctgcg tgcagcagat acggcttcgg caaagtatgc cttgctttta tcgcggctgt
     4801 atgccagtgc ctgtacataa ttgacctttt tgtcaccgag cagatccctg attgctttca
     4861 gcggggtcac ggaattctcc tccctgccgt caaagcacca tgttcc
//
LOCUS       DAOPBK010000172         4891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_14512_length_4891_cov_4.428660, whole genome shotgun sequence.
ACCESSION   DAOPBK010000172 DAOPBK010000000
VERSION     DAOPBK010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14512_length_4891_cov_4.428660"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /gene="rpsA"
                     /locus_tag="PLA17_09370"
     CDS             <1..1351
                     /gene="rpsA"
                     /locus_tag="PLA17_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPK85662.1"
                     /translation="PGSQIDVKPIRDYDVYVGKTMEFRVVKINQEFKNVVVSHKALIE
                     AELEQQKKEIISRLEKGQVLEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRVNHPEE
                     IVQLDQKLNVVILDFDDEKKRIALGLKQLAPHPWDSLSESLQVGDKVKGKVVVMADYG
                     AFVEIAPGVEGLIHVSEMSWSQHLRSAQEFMKAGDEVEAVILTLDREERKMSLGIKQL
                     KTDPWENIEQKYSVNSSHKARVRNFTNFGVFVEIEEGVDGLIHISDLSWTKKIKHPAE
                     FTAIDAEIDVVVLEIDKENRRLSLGHKQLEENPWEVFEDIFTVDSIHEGTVIEVFDKG
                     AIIALPYGVEGFATPKHLVKEDGTQAAVEDKLNFKVIEFNKGAKKIIVSHSRVFEDER
                     KGAETASRRSEAESTRKAARKIKASLEKTTLGDITELQALKDEMEEKEAKSRGKKKK"
     gene            1382..1720
                     /locus_tag="PLA17_09375"
     CDS             1382..1720
                     /locus_tag="PLA17_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK85663.1"
                     /translation="MDFNIDKKQQCTVISIENEKLDTHIAPSLKSELVLISGKGEKNI
                     ILDLKKCQYCDSSGLSAILVANRLCKNAGGTFVLCGLNEAVERLITISQLDTVLSITA
                     DTEEAEKLIS"
     gene            1735..2649
                     /locus_tag="PLA17_09380"
     CDS             1735..2649
                     /locus_tag="PLA17_09380"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPK85664.1"
                     /translation="MAFRLTILGTSSALPTSDRYPTAHVLNVHERLFLIDCGEGTQMQ
                     MRRYRIRFGRINHIFISHLHGDHFFGVYPLLSTYNLMGRKTPLNLYAPAPAEELITRH
                     LADFDINLGFDLAVHTVSGRSLKQVLDDKRVEVFSFPLKHRIPTYGYLFREKMPDRNM
                     IKEKITEYGLSIEEIATARRGGDIIRENGEIIPCTEVTMEPRRPASYAFCSDTGWFKK
                     LPSYVKGVDLLYHEATFGADNDALAVKTLHSTASHAARVALEAGAGRLLLGHFSSRYR
                     STTMLEDEARNIFPATEAAREGNTYEIG"
     gene            2822..4426
                     /locus_tag="PLA17_09385"
     CDS             2822..4426
                     /locus_tag="PLA17_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPK85665.1"
                     /translation="MTKRLILTIVAALLLQGTALRSQGLTRETFKLGKTLALLETIYV
                     DSVNISALTEQMIINTLRNLDPHSVYIPAKDVQEENEPLQGNFEGIGIQFNLLNDTII
                     IISPIPGGPSEKVGIIAGDRIIFIAGEKVAGMGISTTGVQKRLRGPKGTKVDVLIFRK
                     GEKELLDFTITRDKIPVNSLDAAYMVTNNVGYIKLSRFSEQTAVEFSQAVEKLKMRKM
                     EHLVIDLRGNSGGYMIPAVQIADELLKDEELIVYLEGLHTSRQEYRSMPSGELTRARV
                     AILLDEGSASASEILAGAVQDWDRGVIIGRRSFGKGLVQNRFPLPDGSEVRLTVARYY
                     TPSGRSIQTPYDEGFDKYFQTFYSRYFNGELIHPDSLNLPDSLKALTIRNKRVVYGGG
                     GISPDIFIPVDTTYYSDYYRDLIRTSTLTNYMLSYTDRNRSDIARRYRSFDLFSREYE
                     IDAAAIEGLKAAGEKNGVRFDQTQYNLSAPEIKSVMKALIARDIWDMNEYFMVINEND
                     QAISKALEVLNDSQLYETLLGYRKPQ"
     gene            4426..>4891
                     /locus_tag="PLA17_09390"
     CDS             4426..>4891
                     /locus_tag="PLA17_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HPK85666.1"
                     /translation="MKLLAKTHYGLEQVLAEELKGLGASGIAVLNRAVAFEGSKLLLY
                     TVNYCSRLALSVLMPVSSFGISGQKDLYNGAVAIEWDRYLDPGKTIAVTAVVNSPHFS
                     HSGYAALLVKDAIADFFRRLTLRRPSVDAKDPDLLVNVHISNNRVTISIDSTV"
BASE COUNT         1307 a         1129 c         1296 g         1159 t
ORIGIN      
        1 tcccggatca cagattgatg tcaagccgat ccgtgattat gacgtatatg ttggcaagac
       61 catggaattc cgtgttgtga agatcaacca ggagttcaag aacgtggttg tgtcacacaa
      121 ggctctcatt gaagccgagc ttgagcagca gaagaaggag atcatctcca ggcttgagaa
      181 gggccaggtg ctcgagggta ctgtcaagaa catcacctca tatggtgtat tcattgacct
      241 cggcggtgtt gacggactta tccatattac cgatctctca tggggcaggg tcaaccatcc
      301 cgaggagatc gttcagcttg atcagaaact caatgttgtt atcctcgact tcgacgatga
      361 aaagaagagg atcgccctgg gtctcaagca gcttgccccg catccgtggg attcgctcag
      421 cgaaagcctt caggttggag acaaggtcaa gggtaaggtt gttgtcatgg ctgattacgg
      481 tgcgtttgta gagatagccc ctggtgttga agggcttatc cacgtgtcgg agatgtcatg
      541 gtcacagcac ctgcgcagtg cacaggagtt catgaaggca ggcgacgagg ttgaggctgt
      601 catcctgacc cttgaccgcg aggagcgcaa gatgtctctc ggcatcaagc aactcaagac
      661 tgatccatgg gagaatatag aacagaaata cagtgtcaac tcaagccaca aggctcgcgt
      721 tcgcaacttc accaacttcg gtgtatttgt agagattgaa gagggtgttg acggcctgat
      781 tcatatttca gacctctcat ggacgaagaa gataaagcat cctgccgagt tcaccgccat
      841 tgacgcggag attgacgttg tggtcctcga gatagacaag gagaacagga ggctgagcct
      901 cggtcacaag cagcttgagg agaatccgtg ggaagtattt gaagatatct tcacagttga
      961 ctccattcat gagggaacag tcattgaggt ctttgacaag ggcgccatca ttgcgctgcc
     1021 ttacggtgtt gaaggttttg caactcctaa acatcttgtg aaggaagacg gtacccaggc
     1081 tgctgtggaa gacaagctca acttcaaggt gattgaattc aacaagggag ccaagaagat
     1141 catcgtttct cacagccgcg tttttgagga tgaaagaaag ggtgcggaaa ctgcctcaag
     1201 gcgaagcgaa gctgaaagca ccaggaaggc agcccgcaag atcaaggcca gccttgagaa
     1261 gacgactctc ggagacataa ctgagcttca ggccctcaag gatgagatgg aagaaaaaga
     1321 agctaaaagc cgaggtaaaa agaaaaaata accttatttt tagttgccaa aataatcagc
     1381 tatggatttc aatattgaca aaaagcagca gtgcactgtt ataagcattg aaaatgaaaa
     1441 acttgatacc catattgcac cttctttaaa gtcggagctg gtacttattt caggcaaggg
     1501 agagaagaat atcattcttg accttaagaa gtgccagtac tgtgattcgt cgggtttgag
     1561 tgccatactg gtagcaaaca ggctctgcaa gaacgccggc ggtacttttg tgctgtgtgg
     1621 gctgaacgag gctgtggaga ggctgataac catctcgcag cttgatacag ttctctccat
     1681 aaccgctgat acagaagagg cagagaaact catctcctga gaagaggcag ctgcatggca
     1741 ttcaggctga ccatactggg tacaagctct gctctgccca cctcagaccg atatccgacc
     1801 gctcatgtgc tcaatgtgca tgagcggttg tttttaattg actgtggcga gggcacgcag
     1861 atgcagatgc gacgctacag aatccgtttc ggacggatca accatatttt tatcagtcat
     1921 cttcacggtg atcacttctt cggggtttat cccctgcttt ccacctataa ccttatgggg
     1981 aggaagacgc cgcttaacct ttatgcccct gctccggcag aggagttgat aaccaggcat
     2041 ctggccgatt ttgacatcaa tctcggattt gaccttgccg ttcacacggt ctcggggcga
     2101 agcctgaagc aggttcttga cgataagcgg gtggaggtat tctcttttcc gctgaaacac
     2161 cgcataccga catatgggta ccttttcagg gagaagatgc ctgaccgcaa tatgataaag
     2221 gagaagatca cagaatacgg gctttcaatt gaagagatcg ccaccgcgag aaggggtggt
     2281 gatataatcc gtgagaatgg cgaaatcata ccttgcaccg aggtcaccat ggagccccgc
     2341 cgaccagcct catatgcctt ctgcagtgat accggctggt ttaagaaact gccgtcatac
     2401 gtgaagggtg ttgatcttct ttaccatgaa gccaccttcg gagccgacaa tgatgcactg
     2461 gcggtcaaaa ccctccactc tacggccagc catgctgcca gggtagcact ggaagcggga
     2521 gcaggcagac tgttgctcgg ccacttttct tcccgttacc ggtcaacgac aatgctggag
     2581 gatgaagcgc gaaatatctt tcctgctacc gaagctgcac gggaaggtaa tacatatgag
     2641 atcggctgat aacctggtaa caacagatta attatctttg actctttgat aaccacctta
     2701 ataaatcacg ggtgcagaaa agaatcctga tccctggcct gtcggatggc atttactcct
     2761 ctgttgcgtc gccgaagtcc tggaatggaa attgaagtga agtcaagaga aaaaaacgga
     2821 aatgacaaag agacttattc tgacaattgt tgctgccctg ctgcttcagg gcactgctct
     2881 caggtcacag gggctaaccc gggagacatt caagctgggg aaaacccttg ccctgctgga
     2941 gacaatttat gttgacagtg tcaacatttc agccctgacc gagcagatga taataaacac
     3001 gcttcgtaac cttgaccctc attcggtata tatacctgca aaggatgtgc aggaagagaa
     3061 tgagccgctc cagggcaatt tcgaagggat cgggattcag ttcaacctgc taaatgatac
     3121 gattataatt atctcaccca ttccgggagg accgtcggaa aaggttggca ttatagccgg
     3181 cgacaggata atatttattg ccggtgaaaa ggttgccggc atgggtatct ccaccaccgg
     3241 ggtacagaag agactcagag ggccgaaggg aacgaaggtt gatgtactga tcttcaggaa
     3301 aggagagaag gaactgctcg attttacgat cacccgtgac aagataccgg taaacagcct
     3361 tgatgcggca tatatggtaa caaataatgt gggatacatc aagctcagcc gtttttcaga
     3421 gcagaccgcc gttgagtttt cgcaggcagt tgagaagctg aaaatgagaa aaatggagca
     3481 tctggtgata gatctcaggg gcaactcggg aggttatatg atccctgcgg ttcagatagc
     3541 cgatgagctt cttaaggacg aggaactgat tgtatatctc gagggattgc atacatcgag
     3601 gcaggaatac cgtagcatgc cttcaggaga gcttacaagg gcccgtgttg ccatactgct
     3661 tgacgagggc tccgcctcag ccagtgagat tctggcagga gcggtgcagg actgggacag
     3721 gggtgtgatc atcgggcgcc gcagtttcgg caaaggcctg gtgcagaaca ggtttccgct
     3781 tcctgatggt tcagaggtca ggctcaccgt tgcaagatac tacaccccat cgggccgctc
     3841 catccagact ccatatgacg agggttttga caagtatttc cagacattct acagcagata
     3901 tttcaacgga gagcttatac accctgacag ccttaacctg ccagactcac tcaaggcttt
     3961 aaccatcagg aacaaacggg tggtctatgg aggaggaggc atcagtccgg atatctttat
     4021 acctgttgat actacttatt attctgatta ttaccgtgac cttatcagga cttccactct
     4081 cacgaattac atgctttcct acactgaccg taaccggtca gatattgccc ggcggtacag
     4141 gagttttgat ctattcagca gagagtatga aattgacgct gcagccattg aagggctgaa
     4201 ggcggcaggg gagaagaacg gtgtcaggtt tgaccagacg cagtataatc tatctgcccc
     4261 cgagataaaa tcagtgatga aggctctcat agcccgtgac atctgggata tgaatgagta
     4321 tttcatggtt atcaatgaga atgaccaggc aatcagtaag gcacttgaag tgctgaatga
     4381 ttctcagctc tacgagacgc ttctcggata tcgtaagcca cagtaatgaa gcttcttgca
     4441 aaaactcact atggtcttga acaggttttg gctgaggagc tgaagggcct tggcgcttcc
     4501 ggtattgctg tcctcaacag ggcagttgcc tttgaagggt caaaactgct gctgtacact
     4561 gtcaactact gcagtcgtct tgcgctgtca gtgcttatgc ctgtatcatc attcggcatt
     4621 tccggtcaga aagacctgta caacggggcg gtcgccatcg agtgggaccg ttatcttgac
     4681 cccggtaaaa caattgcggt gacggcagtt gtcaattctc cccatttttc ccattcgggc
     4741 tatgcagccc tgctggtgaa ggatgccatt gccgatttct tccgcaggct gacactcaga
     4801 aggccgtcgg tggatgcgaa ggatcccgac ctgctggtga acgttcacat cagcaacaac
     4861 agggtaacca tatcaattga ctcaacggtg g
//
LOCUS       DAOPBK010000173         4862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_14623_length_4862_cov_5.392969, whole genome shotgun sequence.
ACCESSION   DAOPBK010000173 DAOPBK010000000
VERSION     DAOPBK010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14623_length_4862_cov_5.392969"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PLA17_09395"
     CDS             <1..1042
                     /locus_tag="PLA17_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK85667.1"
                     /translation="ELQLAEAGLPETDVPGRATKYSAKGMMAKLYLYQKDYTNARAKA
                     WEVMESGQYDLFPDYRAMFNSSAHNNNIETLFAVQHQCTMNPWGAGNQLNPDRGPSSL
                     QTDEANMWELFRPSMDIRTAYEPNDKRRAGSMMEHGWTMPQWKPQKLPQADGSFTADD
                     AAYNEFMKDGYRYDTIQDVKQGGTLNGTRSTIAKYVVGPGQKYGGEQVIGMNTGINFM
                     MLRYADILLIYAEATLGEAASTNDATALEAFNKVRLRAGLPVKEVLTLDDIIKERRVE
                     FAFEGDYWFDITRLGFAKAKQIIEAQNRGTVAGVVRVTGFTEDKMFLPIPASEVLQDP
                     LLNEDPQPYYTK"
     gene            1146..2378
                     /locus_tag="PLA17_09400"
     CDS             1146..2378
                     /locus_tag="PLA17_09400"
                     /inference="COORDINATES: protein motif:HMM:NF037325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycan-binding surface protein"
                     /protein_id="HPK85668.1"
                     /translation="MMKKIINNTLSALLLLLLTGAAGMVFQACEGNEEDMPMPEIFYI
                     RKADPAKSDSLVTHAFMGDNIVIIGRNLGNVDEVWFNDQKAGLNPNLVTPTTIIVSVP
                     QVIPSTVTNYMYLINMDKKDTLKYSFGVDVPAPQVDRMVCEYVADGETAVIRGNYFVD
                     DPASPLTVLFPGNVPGTIVSFTINEIQVTVPSGVGPGQIQVKSLYGTTRSRFFFRDDR
                     NIILNFDDLTAAGGWRSGVMGNSNPAGVSGNYVRFSGALAGGTGVTWNEDGFSFNLWP
                     QANGRPDEPVYTGELKDAVIKFEINVVEAWESCALQMIFTPYSVTGTNSYIADGSVPR
                     GLWNPWKATGTYKTDGWETVSYPLSEFIYSNDGSVCANKLTADMISGLTFFVWHGGVA
                     GKDCNPHICIDNIRVVPK"
     gene            2402..4534
                     /locus_tag="PLA17_09405"
     CDS             2402..4534
                     /locus_tag="PLA17_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPK85669.1"
                     /translation="MDKIQIKFRNWALLFATICVVGAGLLFTSCEGKEEEDTRVVLQS
                     FGPMPIARGAELKFIGLNLDRVTAVILPENISITSFTKKEARLLTLTVPQEAVPGYVV
                     LKTPDGDITTKTMIGFSEPVSIAGFTPATVKAGDELTITGDYLNLVKEVILTDRITVA
                     EDDFISHSRTEIKLTVPAEAQTGKIAVSNGDEEPIIVYSASTLTVTLPAFTTVTPNPV
                     KAGTNLTIAGTNLDLVLSVRLGGGKVIEAGDFVSHSATQIVLTVPADTKDGKVILVPA
                     SGVEVSTETDLVMVVPTLSVTPVTLKNGADITVTGTNFDLIDHVIFGGNKQGTIKAGG
                     TATQILVTVPDDAVDGVVTFVTKADKEVTGPNLTMIVPAFSSFSPTSARANTTITISG
                     TDLDLVTKVIFTGDLEGAIGARTETSLAVTVPVGARTGKITIETKNGTRVVSAIDFTL
                     QANLPTFGSYTEFRGEPGKILTINGTNLLLVKELIFPGNVPATAYGVKTDTRIEVYVP
                     MNVTRGYGTIALLTYEGEQGIFPQIFFGATDPVVDPALMINDFEVGADGHDLGWDNWG
                     GAVELGNDPAIAISGNYMHGVLPALNGWTWIWGCNHSQLPKPSVTKADHYLKMDVNIT
                     RPFAPGTGSFTMKLAGTDIDIGHLGIQNSDGSWSTPGWITITFDLATYEALPAVIPSS
                     GDWGMTLWTGVDMDLTGLYVDNIRFEHK"
BASE COUNT         1309 a         1187 c         1254 g         1112 t
ORIGIN      
        1 ggagttgcag cttgctgagg caggcctgcc cgaaacggat gtccccggca gggcgacaaa
       61 gtattcggcc aaggggatga tggcaaagct ctatctctat caaaaggatt ataccaacgc
      121 cagggcaaaa gcatgggagg taatggagtc agggcagtat gatctcttcc ccgattaccg
      181 cgccatgttc aactcaagcg cccacaacaa caacatcgaa acactctttg cggttcagca
      241 tcagtgtaca atgaacccat ggggagcagg caaccagctc aatcctgacc gcggaccctc
      301 aagccttcag actgacgaag caaatatgtg ggagcttttc agaccatcaa tggatatcag
      361 gacggcttat gaacccaatg acaagagacg tgcaggctca atgatggagc atggctggac
      421 catgcctcaa tggaagccgc agaagcttcc gcaggctgac ggctcattca ctgctgacga
      481 tgctgcttac aatgaattca tgaaggatgg ctacaggtat gataccatcc aggatgttaa
      541 acagggaggc acactgaacg gaacacgttc aaccattgca aaatatgttg tagggccagg
      601 ccagaaatat ggtggtgagc aggtcatagg catgaatacc ggtatcaatt ttatgatgct
      661 gcgctatgcc gatatacttc ttatctatgc tgaagctacc ctgggtgaag ctgcatcaac
      721 caatgacgca acagcacttg aggcgttcaa taaggtgcgc ctccgtgctg gtttgccagt
      781 gaaagaggtt ctgacacttg acgatatcat aaaggaacgc cgcgtggaat tcgcctttga
      841 aggtgattac tggtttgaca tcacccgtct gggttttgca aaggccaaac agatcattga
      901 agcacaaaac aggggaacgg tagcaggtgt tgtccgtgtt acaggattta ccgaagacaa
      961 gatgttcctc ccgatcccgg catcggaggt gcttcaggat cctttgctca acgaagatcc
     1021 gcaaccctat tacaccaaat agagatacat gatatgaacc acgcatgtta ccaggaaaga
     1081 ttaagttaca ggcttatctg caatagatgc gggttctgtc atacctttaa tatcaaagtt
     1141 gtattatgat gaaaaagata attaacaata cattatcggc attattgctg ctgctcctga
     1201 cgggagccgc aggaatggtg ttccaggcat gcgaaggcaa tgaggaagat atgccgatgc
     1261 ctgagatctt ctacatccgc aaggcagacc cggccaagtc agactcactg gtgacccatg
     1321 ctttcatggg tgacaacata gtcataatag gccggaacct tggcaatgtt gacgaggtct
     1381 ggttcaatga ccagaaggca gggctgaacc ccaacctggt cacgccaacc acgattatcg
     1441 tttcagtacc gcaggtaata ccgtcaacgg ttaccaatta catgtacctg atcaacatgg
     1501 acaagaagga tacgctgaag tatagtttcg gcgttgacgt tcccgctccg caggttgaca
     1561 ggatggtttg cgagtacgtg gctgacggtg aaacggcagt gatcaggggc aactactttg
     1621 ttgatgaccc ggcttcaccg cttacggtgc tcttccccgg aaatgtcccc ggaaccatcg
     1681 tgagttttac gatcaatgag attcaggtga ctgttccatc aggtgtgggt cccggccaga
     1741 tacaggtcaa atcgctctac ggtaccaccc gttcacgctt tttcttccgt gatgacagga
     1801 acatcatcct gaactttgat gacctgacag ctgccggcgg ttggcgttcg ggagtaatgg
     1861 gcaattcaaa ccctgcagga gtttccggaa attatgtgag gttttcgggc gcactggcag
     1921 gcggtacagg tgtaacatgg aacgaggacg gattcagctt caacctctgg ccgcaggcaa
     1981 acggcaggcc ggatgagcct gtttataccg gcgaactgaa ggatgctgtg atcaagttcg
     2041 agatcaacgt tgttgaagca tgggagtcat gcgctctgca gatgatcttc actccatatt
     2101 ccgtcacggg aacgaacagc tatattgcag atggttcagt gccccgtggc ctctggaatc
     2161 catggaaggc aacaggcacc tacaagactg acggctggga gacagtatca tacccgctct
     2221 cagagttcat ctacagcaac gacggctcag tctgtgccaa caagcttact gctgatatga
     2281 taagcggtct gacattcttt gtctggcacg gcggtgtggc aggcaaggac tgcaatccgc
     2341 acatctgcat agacaacata cgcgttgtac cgaagtaaca tcaaccgaaa aaagaattgc
     2401 aatggataag attcaaataa aattccgcaa ttgggcgctt ctcttcgcga caatatgcgt
     2461 ggtgggcgcc ggtctactgt tcacctcatg cgaggggaag gaagaggaag ataccagggt
     2521 ggttctccag agcttcgggc caatgcccat tgccagagga gctgaactga aattcatcgg
     2581 gctgaacctt gacagggtga cggcagtgat tctgcctgaa aatatctcaa ttacttcatt
     2641 cacaaagaag gaagcccgac tgctgacact caccgtaccc caggaagccg tgcccggtta
     2701 tgtggtgctg aaaactcctg acggtgatat cacaacaaaa accatgatcg gtttctcgga
     2761 acctgtctcc atagccggct tcaccccagc gacagtcaag gcaggcgacg agctgacaat
     2821 cacgggcgat tacctgaacc tcgtgaagga ggtgattctc accgacagga tcacggtagc
     2881 agaggatgat ttcataagtc actcgcgcac cgagatcaaa ctgacagtgc cggcagaggc
     2941 acagaccggc aaaatagctg tttcaaacgg tgatgaagag ccgataatag tctactcagc
     3001 atcaaccctt acggtgaccc tgcctgcatt tacaaccgta actccgaatc ctgtcaaggc
     3061 cggcaccaat ctcacgattg ccggtaccaa ccttgacctg gtgctctcag tcaggctcgg
     3121 cggcgggaaa gtgattgaag ccggggattt cgtctcgcac agcgcaacac agattgttct
     3181 gaccgtaccg gctgatacga aggatggcaa ggttatactg gtaccggcat caggtgtaga
     3241 ggtgagtaca gagactgacc tggtaatggt tgtcccgact ctctccgtca ccccggtgac
     3301 gcttaagaac ggtgctgaca taactgtgac cggaaccaat tttgacctta ttgaccatgt
     3361 cattttcggc ggaaacaaac agggaacaat caaggccggc ggcacagcca cacagattct
     3421 agtgacggtt cccgatgatg ctgtcgacgg tgttgtgacc ttcgtaacga aggctgacaa
     3481 ggaggttaca ggccccaatc ttacgatgat cgttccggca ttctcatcgt tcagtcccac
     3541 ttcggcaagg gccaacacca ccatcacaat ttcaggtact gatcttgacc tggtgaccaa
     3601 agttatattc accggtgacc ttgaaggcgc cataggggca agaacggaaa catccctggc
     3661 agttacagtc cctgtgggag ccagaacagg caagattacc attgagacca aaaacggcac
     3721 ccgggtggta tcagctattg acttcacgct gcaggcaaac ctgccaacct tcggatctta
     3781 caccgagttc cgtggtgaac ctggcaagat actgaccatc aatggtacaa acctgcttct
     3841 ggtcaaggag ctcatattcc ccggaaacgt ccctgcaaca gcctatggcg tcaagaccga
     3901 caccaggatt gaagtttatg ttccaatgaa tgttacgcgt ggatacggta ccatcgctct
     3961 tcttacctat gagggtgagc agggcatctt cccgcagatt ttcttcggcg ctactgatcc
     4021 ggtggttgat ccggcactca tgatcaatga ctttgaagtc ggagctgacg gtcacgacct
     4081 tggctgggat aactggggcg gagccgtcga gcttggcaat gacccggcaa tagcaatatc
     4141 aggtaactac atgcacggtg tgctgcctgc acttaacgga tggacatgga tatggggctg
     4201 taaccactca cagttgccca agccgtcagt aaccaaggca gaccactacc tgaagatgga
     4261 tgtaaatatt accagaccat ttgctccggg aacaggttcg ttcacgatga aactggccgg
     4321 aacggatatt gacataggcc atctcggcat tcagaacagt gacggaagct ggtcaacacc
     4381 aggctggatt acaattacat ttgacctggc aacatatgag gcacttccgg cagtgatccc
     4441 gtcatcggga gactggggca tgaccctgtg gaccggggta gatatggacc tcaccggcct
     4501 ctatgttgac aatatcaggt ttgaacataa gtagtggtga ctgattaggt cagccattta
     4561 accagtagcg aagggtatct cggattttat cactaaattg agataccctt tgcatttgat
     4621 aacagatcgg taaatttttg ctgacagata catgatcaat taataggaat atttattcta
     4681 gtaaagatga tcaatcttga taatcaggca gttataagca atcatttgaa tagtatacag
     4741 aacaattaat ttaataacag ggtaactttt ttacatgatg atatttattc ccgggctgct
     4801 ggcattcata tcactacttg gcgcatgccg gatgggtgga tttgcacagg gcacaaaata
     4861 cg
//
LOCUS       DAOPBK010000174         4846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_14674_length_4846_cov_4.782718, whole genome shotgun sequence.
ACCESSION   DAOPBK010000174 DAOPBK010000000
VERSION     DAOPBK010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14674_length_4846_cov_4.782718"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLA17_09410"
     CDS             complement(<1..1035)
                     /locus_tag="PLA17_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPK85670.1"
                     /translation="MMTRITAIIIVSVMMAACGSKTTKPDTAQNGWLVTPKLHSVTVN
                     DSFWKPLIERNRTVTIPYAFGKCEETGRIDNFAIAGGLKKGKHTGARYNDSDVFKIIE
                     GACYSLLETPDPALRHYVDSLVTLIAAAQEDDGYLYTTRTIDPVNMAPGAGKERWIDE
                     RVSHELYNVGHMYEAAVAHYLATGSNSFLNVALKNAELVASEFGWGLREIAPGHQEIE
                     IGLIKLYELTGNDRWLELARFFIDVRGKQEEYTRHPAGSRFEVYNDSVYLQMHLPVLE
                     QTEAVGHAVRGAYLYTAMADLSRVTGDNSYLEASEKIWHDVIRGKIYITGGIGSKEYG
                     EAFGEAFELPN"
     gene            complement(1032..2702)
                     /gene="araB"
                     /locus_tag="PLA17_09415"
     CDS             complement(1032..2702)
                     /gene="araB"
                     /locus_tag="PLA17_09415"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPK85671.1"
                     /translation="MMHNYTIGVDFGTLSGRAVLVDTTTGEEVAVAVHEYSHGVMDEK
                     LPDGTPLGVDWALQHPQDYLDVFRTTIPMVMKMSMVKPDQVVGVGIDFTACTVLPVKA
                     DGTPLCFLEEFRKRPNAWVKLWKHHAAQDEANRLNAIAEERNEKFLKRYGGKISSEWM
                     IPKIWQTVNEDPEVYDAADRFIEAADWVVWQLTGRETRNTCTAGYKAIWHKHEGYPSN
                     DFFKALHPKMEKLVEQKLLRTLLPVGTKAGTITAAAAALTGLKEGTAVAVANVDAHVS
                     IPAVGITSPGKMLAIIGTSTCHMLLGNVEREVPGMCGVVEDGIIEGFFGYEAGQSCVG
                     DHFQWFVGNCTPAEYKAEADAAGIDLHTLLTEKAAKLKPGESGLVALDWWNGNRSVLV
                     DVDLTGMIAGMSLITRPEEIYRALIEATAFGTRMIIETFEENGVPVKEFYAAGGIAEK
                     NPFVMQIYADVINREIKISGSPQAPALGSAMFGAVAAGKAAGGFDTIMEAAAVMAKVK
                     EHTFSPIKENVAVYDKLFAEYRLLHDYFGRGANDVMKRLKNIRKEVRG"
     gene            complement(2719..3408)
                     /locus_tag="PLA17_09420"
     CDS             complement(2719..3408)
                     /locus_tag="PLA17_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HPK85672.1"
                     /translation="MLEKLKEDVFRANLDLVRYGLVIMTWGNVSGIDRESGLMVIKPS
                     GVSYDRMTAADMVVTDLDGNVMEGTLNPSTDAPTHRALYRAFPWAGGIVHTHSTYATA
                     WAQACRPVPCLGTTHADHFYGAVPVTRELTEKETADDYEYNTGLIIAEALKGSDPMSM
                     PAVLVASHGPFTWGATPADAVHNAVVLEEVARMAAISAILADPKEIDRHLLDRHYLRK
                     HGKNAYYGQKK"
     gene            complement(3458..4162)
                     /locus_tag="PLA17_09425"
     CDS             complement(3458..4162)
                     /locus_tag="PLA17_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPK85673.1"
                     /translation="MAHIYSRQPKHYLAVDCIIFGYDIAEKELKLLLIKRSFEPALGQ
                     WSLAGGFVQQNESLDDAACRVLCTLTGLSDLYMEQLYTYGEVNRDPGARVISTAYFAL
                     IGIHDLDPEIQKKNGAHWRSISNLPDLIFDHRQMVDKALRELVEKVKVYPVGFELLPE
                     KFTLVQLQNLYEAIYLRPVDKRNFRKKILSMELLDKQDDKERETSKKGAWYYRFNLEK
                     YKELARNGFFFNLEVS"
     gene            complement(4165..>4846)
                     /locus_tag="PLA17_09430"
     CDS             complement(4165..>4846)
                     /locus_tag="PLA17_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HPK85674.1"
                     /translation="KAFTTNFEDLHGLVQLPGLAVQRLMAEGYGFGAEGDWKTAALVR
                     LMKVMAEGLKGGTSFMEDYTYHLDPKNMAILGAHMLEVCPSIAAGKPSLEVHPLGIGG
                     KADPARLVFRTNPGEAIAASLVEMGDRFRLIVNTMKVIECPPMPKLPVASVLWKPYPD
                     LKRAATAWIYAGGAHHTSFSQALTAEHMADFADMTGIEYVLIDENCEIGQFRKELRWN
                     DLCYALKK"
BASE COUNT         1065 a         1370 c         1136 g         1275 t
ORIGIN      
        1 attggggagc tcgaaggctt cgccgaaggc ttctccatat tccttggaac ctattcctcc
       61 ggtaatatat atcttgccgc gtatcacgtc gtgccagatc ttttctgacg cctcgaggta
      121 gctgttgtct cctgtgacac gtgacaggtc agccattgcc gtgtacaaat atgcgccccg
      181 cacagcatgc cccaccgcct cggtctgttc gagcaccggc aggtgcatct gcaggtaaac
      241 cgagtcattg taaacctcga aacgtgatcc tgcagggtgc ctggtatact cttcctgttt
      301 gcccctgaca tcaatgaaaa accgggccag ctcaagccag cggtcattgc ctgtcagttc
      361 atagagtttt attagcccta tctctatctc ctggtgaccg ggggcaatct cacgcagccc
      421 ccagccaaac tccgaagcta ccagctccgc attcttcagg gctacgttca ggaaactatt
      481 tgagcctgtt gccaggtagt gcgccacggc agcctcatac atgtgcccca cattgtatag
      541 ttcatggctt acccgctcgt ctatccatct ctcctttcct gccccgggcg ccatattgac
      601 cgggtcgatg gtacgtgtgg tgtagagata accgtcgtcc tcctgtgctg cagcaataag
      661 tgtaacaaga ctgtctacat agtggcggag cgcggggtct ggtgtctcga gaagtgaata
      721 acaggcccct tcaattatct tgaaaacgtc tgaatcattg tagcgtgcac ctgtatgctt
      781 ccccttctta agtccgccgg ctatggcgaa gttgtcaatg cgccctgtct cctcacactt
      841 gccaaaggcg tatggtattg taaccgtcct gtttctttcg atgagcggct tccagaaaga
      901 atcattcacg gtaactgagt gaagcttagg cgtcaccagc cagccgttct gcgccgtatc
      961 cggtttggtg gttttgcttc cgcatgcagc catcatgact gaaacaatta ttattgcagt
     1021 gattcttgtc atcatcccct gacctctttt cttatgttct tcagtctctt catcacatca
     1081 ttggctccac ggccaaagta atcatgcaga agcctgtatt cagcaaagag tttgtcatat
     1141 acagcgacgt tctctttgat gggactgaac gtatgctctt tcacctttgc catcacggca
     1201 gctgcctcca taatggtgtc aaagccgcct gcagccttac ctgcagccac cgcaccaaac
     1261 attgccgagc ccagtgccgg tgcctggggt gagcccgata tctttatctc cctgttgatt
     1321 acgtctgcat agatctgcat cacaaacgga ttcttttcgg cgataccgcc ggcagcataa
     1381 aactctttca ccggaacgcc gttttcctca aatgtctcta taatcatcct tgtaccgaat
     1441 gcggtagcct ctatcagcgc cctgtatatc tcctcaggtc tggtgataag gctcatgccg
     1501 gcaatcattc ccgtcaggtc aacatcaacg aggaccgacc tgttgccatt ccaccagtcg
     1561 agtgccacca gtccgctttc accaggttta agctttgcag ccttttctgt aagcagggta
     1621 tgaagatcaa tgcctgccgc gtctgcctct gccttgtatt cagccggtgt gcagttccca
     1681 acaaaccact ggaagtggtc acccacacag ctctgtccgg cttcgtagcc aaagaatccc
     1741 tcgatgatgc cgtcctcaac tactccgcac atgcccggca cctccctttc gacgttaccc
     1801 agaagcatgt ggcaggttga tgtgcctatg atggcaagca tcttcccggg tgatgttatt
     1861 cccactgccg gaattgatac atgggcatca acgttggcca cggcgacggc tgtgccctcc
     1921 ttcagcccgg tcagtgcagc ggctgcggca gtgatggttc ctgcctttgt gccaaccggc
     1981 agaagggtcc gcagaagctt ctgttcaacc agcttctcca tcttcgggtg aagggctttg
     2041 aagaaatcat ttgaaggata gccttcatgc ttgtgccata tggccttata tccggcagtg
     2101 caggtattgc gtgtctccct gccggtgagc tgccatacca cccagtcagc cgcttcaatg
     2161 aaacggtcag cagcatcata tacttcagga tcttcgttaa cggtctgcca gatcttgggt
     2221 atcatccatt ccgaggaaat ctttccgccg taacgtttca gaaacttctc atttctctct
     2281 tcagctatag cgttcagcct gttggcctca tcctgggcag cgtgatgctt ccatagcttg
     2341 acccatgcgt tgggcctttt cctgaactct tcgagaaaac agagcggggt gccgtctgcc
     2401 ttcaccggca acacagtgca ggcggtgaag tctattccca cgcccaccac ctggtcaggt
     2461 ttaaccattg acatcttcat gaccatgggt atggtagtcc tgaatacatc caggtaatcc
     2521 tgcgggtgct gcagcgccca gtcaacgccc agcggagtgc cgtcagggag cttctcgtcc
     2581 atgactccat gagaatattc gtgaaccgca acagctacct cttcacctgt tgtagtatca
     2641 accaggaccg ctcttcctga aagggttcca aaatcaactc ctatagtata attgtgcatc
     2701 atgatttatt tgaatgaact attttttctg accgtaatat gcgttttttc cgtgtttgcg
     2761 caggtaatgc ctgtcaagca ggtgcctgtc aatttctttt ggatctgcca gtatggctga
     2821 aatggccgcc atgcgtgcaa cctcctcaag aacaacggca ttgtgtactg cgtcggcagg
     2881 cgtggcgccc catgtaaacg gcccgtgcga ggcaaccagg actgccggca ttgacatggg
     2941 atcactcccc ttcagcgcct cggcaataat cagtccggta ttatactcat aatcatctgc
     3001 tgtctccttt tcagtcagct ctcttgtgac gggaacagcc ccgtaaaaat gatccgcgtg
     3061 tgttgtgcca aggcatggta ccggcctgca agcctgtgcc catgcagttg cataggtgga
     3121 atgggtgtgc actatgccgc ctgcccacgg gaaagccctg tagagtgccc ggtgagtggg
     3181 tgcatccgta gaaggattca gggtcccttc catgacattc ccatcaaggt ctgttacaac
     3241 catgtcagct gccgtcattc tatcatatga aacccctgac ggcttgatta ccatcagccc
     3301 tgattcacgg tctattccgc tgacgttgcc ccaggtcatg atgaccaggc catatctgac
     3361 cagatcaaga tttgccctga atacatcctc cttaagcttt tcaagcatga tcaatgcgtg
     3421 tttatggtta aatccgctgc cttagcatca aatggtgtca gctcacctcc aggttaaaga
     3481 aaaaaccgtt tctggcaagc tccttgtatt tctcaagatt gaacctgtag taccaggctc
     3541 cttttttcga agtctccctt tccttatcat cctgtttatc aagaagctcc attgagagaa
     3601 ttttctttct gaagttccgt ttgtctaccg gcctgaggta aatggcttca tagaggttct
     3661 gcaactggac cagggtaaac ttttcaggca ggagttcaaa tcccaccggg taaactttaa
     3721 ccttttccac cagttcacgc aatgccttgt ctaccatctg cctgtgatca aagatcagat
     3781 cagggagatt tgagatggac ctccagtgag cgccattctt cttctgtatc tcagggtcaa
     3841 ggtcatggat cccaatgagg gcgaaataag ctgtagagat aaccctggca cccgggtcac
     3901 ggttgacctc gccgtaggtg tagagttgct ccatgtacag gtctgacagg cctgtcagtg
     3961 tgcaaagcac cctgcatgca gcatcatcaa gactttcgtt ctgctgcacg aatccccccg
     4021 ccagcgacca ctgcccgagt gcaggctcaa agcttctctt tatcaggaga agcttgagct
     4081 ccttctcggc aatgtcatac ccgaagatga tacagtctac cgccaggtaa tgcttcggtt
     4141 gtcttgaata gatatgagcc ataattattt tttgagagca taacagagat cattccatct
     4201 cagttccttc ctgaactgcc ctatttcaca attctcatca atcaacacat attcaattcc
     4261 tgtcatatca gcaaaatcag ccatatgctc ggctgtgaga gcctggctga aactggtatg
     4321 atgggctcct ccggcataaa tccatgcagt ggcggctctc ttcagatccg ggtaaggctt
     4381 ccacagcaca ctggcaacgg gaagcttcgg catcggcggg cattcaataa ccttcatggt
     4441 attgacgatg agcctgaacc tgtcacccat ctcaactaga gaagcagcga tggcttcgcc
     4501 ggggttggtc ctgaagacca gtcttgcagg gtcagccttt ccgcctatac ccaggggatg
     4561 gacttccagt gacggcttcc cggcagctat cgaaggacaa acctccagca tatgtgcacc
     4621 gaggatggcc atgttcttag gatcaaggtg atatgtgtaa tcttccatga atgaggtgcc
     4681 tcccttgagg ccctctgcca tgactttcat caggcgaacc agcgctgcag tcttccagtc
     4741 accctctgcc ccgaagccgt agccctcagc catgagtctt tgcacagcca ggccaggcag
     4801 ctggacgaga ccgtgaagat cctcaaagtt ggtggtaaaa gccttg
//
LOCUS       DAOPBK010000175         4755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_14966_length_4755_cov_5.215319, whole genome shotgun sequence.
ACCESSION   DAOPBK010000175 DAOPBK010000000
VERSION     DAOPBK010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14966_length_4755_cov_5.215319"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLA17_09435"
     CDS             complement(<1..517)
                     /locus_tag="PLA17_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HPK85675.1"
                     /translation="MKKINFLIAVALLAAVSCSLSRQDDLTILVDPFIGTAAHGHVFP
                     GATTPFGMVQLSPDNGTSGWDWCSGYNYSDTVIAGFSHTHLSGTGIGDLCDILFLPSM
                     EAFNLSLPDSSASLAKLNRVWFSHDAEQSRPGYYAVALAPGVNAELTATPRCGLQRYT
                     AVEEGTMHLLVD"
     gene            complement(524..3148)
                     /locus_tag="PLA17_09440"
     CDS             complement(524..3148)
                     /locus_tag="PLA17_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPK85676.1"
                     /translation="MTRIIRLPALLSLAVLLSCSSGHLIEDKALRNAVADDYLARSTV
                     YSQVRPDLFGMADTISSTAMREAVTFLLSYMPLSDLAVYEPGYLFENAALALRTRSEM
                     PWGNIVPSGIFMNFVLPSRVNNENPDDFRITCYEELKERVNGLTAMEAALEVNRWCHE
                     KVTYQASDSRTSAPLSTMLSARGRCGEESTFTVSALRAVGIPARQVYTPRWAHTDDNH
                     AWVEFWVDGTWHYLGACEPLPVPDRGWFTEPARRAMLVHTKVFGRYDGSENVIRKETL
                     FNEINVLDRYAETKELNVWVTDGEETPVPGAMVGYMIYNYAEFYPLTRLQSDNNGRCS
                     MTTGYGTLLVWADDGNRYGFSLASPEDTLVHVAIFPPEVIKETIPLDIMVPELPEPFP
                     GIDPDLVQMNNLLNSRGDSIRKAYINSWSAGVSADDIARNAGLSADAVSKIVATSMGN
                     YQAIIDFIAGAGEKAGLALRILENISEKDLRDTPASVLADHLDNAPDPAPGSDITLYD
                     RYVLSPRISNELLSPFRSQLKTLPDELITSFISDPASAAAWIDTAIAITKTENHYSVP
                     IIPGAVLRMRTADIRSRNIFFIALCRSAGIPSRLAPGTGRPQYHSAGEWHDVWFTGDT
                     RPSGTSGYVTFVAGSGEIMKQEPEYHVHFTLARVENGRYNTLDYGYGVKISDIPVKIP
                     LDPGIYMLTTGNRDENGNVLASVSFHELKAGEEQQLRIDLRNLPESPMKGEMLNLESS
                     IETFTGENIVLSSLADKGLVMIWVDPGMEPTRHLLNDLPGLKAEFDSWGGDFVFLTDP
                     ERTPGQISGETVTGAPENAIFAADPGLKLLQTLSGEKSRVRSLPVVLYCNSGGEVLFS
                     SEGYRIGTGQQILKKIRK"
     gene            complement(3155..4195)
                     /locus_tag="PLA17_09445"
     CDS             complement(3155..4195)
                     /locus_tag="PLA17_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK85677.1"
                     /translation="MGKKVKNKDIAARLGLSGTLVSLVLNNKADQHGIKKETQERVLA
                     VARQMGYFNAVAEKGESAPVEEKPGIVGMVVPSMNDPFIYEITPYLQKAFSSIGLGFS
                     VFTRDPDDMRFNRLTTSLKKLFSGMILVGNAVDDSLVRTLNRDSYPIVLLEKSIKRMR
                     LNTVSTDRTAGAAVMTQHLAGLGLKNLLIVSGKDTALYDKEMLEELRNTTKKSKSFDS
                     VNFTVAGQPKAGEKFDFQVIENFLRPPHRSDVIIVLNSSLLFPLYMALREKNIRIPQD
                     VALVSMEDGIGFDLLTTPITRLRRPLPAMSLKVVNIIWSEIKNQGKSKYKRQVNMSPE
                     LLVRSSCGSYLK"
     gene            complement(4294..>4755)
                     /locus_tag="PLA17_09450"
     CDS             complement(4294..>4755)
                     /locus_tag="PLA17_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPK85678.1"
                     /translation="FFPTSIELRQQSFLWAKDLSTYDSILDLPFTIPMYGDHVSLFTL
                     LMTASTIISTRISNPSGGQEQMPGMKSMMYLMPVMFMLILNNFSSGLTYYYFLANLIS
                     IIQTQFSRRFVSEKELLKKIEENKKKPVKKSKWQQRLEAAAKQSGRPLPKK"
BASE COUNT         1159 a         1218 c         1141 g         1237 t
ORIGIN      
        1 gatcgacaag cagatgcatc gttccttctt caacggcagt atatctctgc aaaccgcatc
       61 ggggagtggc ggtgagttcg gcgttaaccc ccggagcgag ggccacggca taatatcccg
      121 gtcttgactg ctcggcatca tgagaaaacc ataccctgtt gagctttgcc agcgaggcac
      181 tgctgtcagg caatgaaaga ttgaaagcct ccattgacgg aagaaacaga atatcacaca
      241 ggtcgccaat accggtaccg ctgagatggg tgtgactgaa ccctgcaatc accgtgtcag
      301 agtagttgta tcccgaacac cagtcccatc cggaggttcc gttgtcagga ctgagctgca
      361 ccattccgaa tggggttgtt gctccgggga agacatgccc gtgggctgcg gtgcctataa
      421 acggatcaac aagaatggtg aggtcatcct gccttgacaa tgaacatgaa acagctgcaa
      481 gaagagcaac tgcaatgaga aagttgattt ttttcatacc ggattatttt ctgatcttct
      541 tcagaatctg ctgtccggtt ccaatccggt atccttccga ggaaaagaga acctctccgc
      601 cgctgttgca gtacaataca acaggcaatg acctgacacg ggatttttct ccggaaaggg
      661 tctgcagcag cttaagcccc gggtcagctg caaaaatggc attctccggc gctccggtga
      721 ccgtctcccc ggaaatttgt ccgggagttc tctccggatc ggtaagaaat acaaaatctc
      781 cgccccagct gtcaaattct gctttcagcc ctggcaggtc attgagcaga tgacgggtag
      841 gctccatgcc cggatccacc caaatcatta cgagaccctt atcggccagt gagctgagaa
      901 caatattttc accggtaaaa gtttcaatcg aactttcgag gtttaacatc tcccctttca
      961 tcgggctctc gggaagattc ctcagatcaa ttctcaactg ctgttcttcc cctgctttaa
     1021 gctcatgaaa tgatacagaa gccagaacat tgccgttctc atccctgtta cccgtggtta
     1081 gcatgtaaat tcccggatcc agtgggattt tcaccggtat atcactgatt tttaccccgt
     1141 agccatagtc aagagtattg tatcttccat tttcaaccct ggcaagagta aagtgaacat
     1201 ggtactcggg ctcctgtttc ataatctcac cggagcctgc gacaaatgta acgtaacctg
     1261 atgttcctga aggtcttgta tcaccggtga accatacgtc atgccattct ccggcactgt
     1321 gatattgcgg tcttccggtt cccggcgcca ggcgtgacgg tatgccggcg ctcctgcaca
     1381 gtgcaatgaa gaagatgttc cgtgaacgta tgtcagccgt ccgcatcctg agtacagcgc
     1441 cggggatgat tggcaccgag tagtggttct cagtttttgt aatggctatg gcagtgtcaa
     1501 tccatgccgc tgctgaagca ggatcactga tgaatgatgt gatcagttca tcgggcagtg
     1561 tttttagctg actcctgaag ggtgacaaca gttcattcga tatcctgggc gagagaacat
     1621 atctgtcata cagtgttatg tcacttccgg gagcaggatc aggcgcattg tcaagatgat
     1681 cggcaagcac tgaagcaggt gtgtcacgca aatccttttc agaaatattt tcaagtatgc
     1741 ggagcgccag ccctgctttt tctccggctc cggcaataaa atcgattata gcctggtagt
     1801 tgcccattga tgtcgcgact attttgctga ccgcatccgc tgacaatcct gcattgcgtg
     1861 caatatcatc agcactgaca ccggctgacc aggagtttat atatgccttc cttatactgt
     1921 ctcccctgct gttcagtaaa ttattcatct gcaccaggtc gggatcaatt ccgggaaagg
     1981 gttcaggcag ctcaggcacc ataatgtcaa gaggaatggt ttcctttatt acttccggcg
     2041 ggaagatggc aacatgcaca agcgtatcct cgggtgaggc cagtgagaat ccgtacctgt
     2101 taccgtcatc agcccataca agcaatgttc cgtaacccgt cgtcatactg cacctgccat
     2161 tattgtcgct ctgaagtctt gtcagggggt aaaactcagc gtaattatag atcatgtacc
     2221 cgaccattgc accaggcaca ggagtttctt ctccgtcagt gacccatacg ttcagttcct
     2281 ttgtctcagc atacctgtcg aggacattaa tctcattaaa cagcgtttcc ttcctgatca
     2341 cattctcgct gccgtcatac ctgccaaata ctttggtatg cacaagcatc gccctccgcg
     2401 caggctcggt gaaccagccc ctgtcaggaa cgggcagcgg ctcgcaggcg cccaggtaat
     2461 gccatgtacc gtcaacccag aactcaaccc aggcatgatt gtcatcggta tgggcccacc
     2521 tgggggtgta gacctggcgg gcagggatac ccacggcacg caatgctgaa acggtaaagg
     2581 tagattcctc cccgcacctg ccgcgggctg aaagcatggt cgacagaggt gccgaggtac
     2641 gggagtcgga tgcctgatag gttaccttct catgacacca gcggttaacc tccagagcag
     2701 cttccatggc ggtcaatccg ttgacacgct ccttaagctc ttcgtaacag gtaatcctga
     2761 agtcatcagg attttcattg ttgactcttg acggcagtac aaaattcatg aagatgcctg
     2821 acggaacaat gttgccccag ggcatctcac tccgggtacg gagggccaat gccgcatttt
     2881 caaacaggta accgggctca tagacggcca ggtcactcaa aggcatgtat gaaagcagga
     2941 aggtgactgc ctcacgcatc gctgttgaac tgatagtatc agccatgccg aacaggtcag
     3001 ggcgtacctg agagtacacc gttgatctgg caagatagtc atcagctaca gcattgcgta
     3061 gtgctttatc ctcaataaga tgtccgctgc tgcatgaaag cagcactgcc agcgacaaca
     3121 gagccggcag cctgatgatc cgtgtcataa tggattattt taaataagag ccgcaactgc
     3181 tcctgacaag caattcagga gacatgttga cctggcgctt gtatttactc ttaccctggt
     3241 ttttgatctc tgaccagatg atgtttacca ccttgagcga catggccgga agaggcctgc
     3301 gcagcctggt aatgggtgtg gtcaggagat cgaagccgat accgtcttcc atcgatacca
     3361 gcgccacatc ctgcggtatg cggatgttct tttcccgtag cgccatgtaa agcgggaaga
     3421 gcagcgacga gttaaggacg attattacgt cgctcctgtg aggcggcctc aggaaatttt
     3481 ctattacctg gaaatcaaac ttctcacctg ccttgggctg ccctgccacg gtaaagttaa
     3541 cagagtcaaa gcttttggat ttctttgttg tattcctgag ttcctcaagc atctccttat
     3601 catataaggc agtgtccttt ccggaaacga tcagcagatt cttcagaccc agcccggcaa
     3661 gatgctgtgt catcaccgct gcccctgccg tacggtcagt gctgacggtg ttaagcctca
     3721 tcctcttgat gctcttttcg agcaggacta tcggatagga gtccctgttc agagtcctga
     3781 ccaggctgtc atcaacagcg ttacccacca ggatcatacc gctaaagagt tttttcagcg
     3841 aggtggtgag cctgttgaag cgcatgtcat ccggatctct tgtgaatact gaaaatccaa
     3901 gtccgatact tgagaatgcc ttttgcaggt aaggagtgat ctcatagata aacggatcat
     3961 tcatggatgg gaccaccatc ccgacaattc cgggcttctc ctcaaccggt gcagattccc
     4021 ctttttctgc aacagcgttg aaataaccca tctgccttgc taccgcaaga accctctcct
     4081 gtgtctcctt cttaattccg tgctgatcag ccttgttgtt cagcaccagt gaaaccaatg
     4141 tgccagaaag acctagtctt gcggcaatgt ctttgttttt aacctttttc cccatttctt
     4201 tttagatgaa aactcaaaat tagctttatt tttttagaaa tggatgcttt agagcatatt
     4261 tgttaaaaaa atgtttaaaa gcattatttg ccgctatttt ttaggtaaag gacgtccgct
     4321 ttgttttgcc gcagcttcaa gacgctgctg ccattttgac ttcttaacag gtttcttttt
     4381 gttttcctct atcttcttca gcaactcctt ttcacttaca aaacgccgtg aaaactgagt
     4441 ctggattatc gagatcaggt ttgcaaggaa ataataatat gtcagacccg atgagaagtt
     4501 gttcagtatc aacatgaaca ttacgggcat caggtacatc atcgatttca ttcccggcat
     4561 ctgctcctgc cctcctgaag gattgctaat cctggtggag atgattgtag aagctgtcat
     4621 gagcagtgtg aagagactca catgatcacc atacatcggg atagtgaatg gcagatcaag
     4681 gatgctgtca taggtggaga gatccttggc ccacagaaat gactgctgcc tcagttcaat
     4741 ggaggtcggg aagaa
//
LOCUS       DAOPBK010000176         4741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15026_length_4741_cov_4.508109, whole genome shotgun sequence.
ACCESSION   DAOPBK010000176 DAOPBK010000000
VERSION     DAOPBK010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15026_length_4741_cov_4.508109"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..165)
                     /locus_tag="PLA17_09455"
     tRNA            complement(92..165)
                     /locus_tag="PLA17_09455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(129..131),aa:Arg,seq:tct)
     gene            complement(253..2028)
                     /locus_tag="PLA17_09460"
     CDS             complement(253..2028)
                     /locus_tag="PLA17_09460"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPK85679.1"
                     /translation="MKPRTFVEKALGAPEGAVVFRKPDLILTHDNTASIFQTFKKMGG
                     EKVSDPDQMVIVLDHNAPPADAKLATQYQDIRNIVAGQGISKFYDSGMGICHQMMSNH
                     ARPGMIIVGSDSHTCTAGAFNALAAGIDRTESAGIWKRGETWFLVPPSMRIELTGRLR
                     PGVTAKDLSLWIIGMLGSDGANYMSIEFHGEGVASLSVSERMTVANLASEMGAKNAVF
                     PADKVLQKFIGSNTPGIWADEGAEYKSSISIDLSEVFPLVAAPHHVDNIKSVASVAGT
                     VVHEGLIGTCTNGRIEDLRTAASVLRGAKVKPGFQLHIIPASKEIYLQAIREGIVTDL
                     IEAGANILSPSCGPCLGTGQGIPANGHTVISTANRNFLGRMGNKEASIYLASPATVAA
                     SAITGVITDPRVTKGDELFPYNPPQSLTNTISEGENRKEGNVWNYADVDNLNTDQMFA
                     GKHTYSVMSSDAAAILPLLFGDFDPSFSSNAKAGDIIIAGDNFGCGSSREHPSVGLAH
                     LGVRAVIIKSVNRIFYRSSINQGLPLIVHREAVEAYRAGDAVEIDFDSGMLKVGEKQF
                     RFEPLPPELKAIIEKKGLVNWIKTV"
     gene            complement(2142..3956)
                     /locus_tag="PLA17_09465"
     CDS             complement(2142..3956)
                     /locus_tag="PLA17_09465"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPK85680.1"
                     /translation="MGMTITEKIIAAHSKYDSVKPGDIVNITIDARAARDFGGANVVK
                     NLNSFGLKVDDPERTLFTFDCNPTGSDQKYAVNQHICRQYARGNGLKVYDIDAGIGTH
                     LLIEQGYVWPGATAVSTDSHANILGAVGAFGQGMGDMDIAAAWNSGSVWFKVPASVKL
                     TLKGIMPDGVTAKDVILNLLRIYGANTLLGYSIELYGDAVDQMSLDERITIASMGTEM
                     GAIIILFPPTPAIVEYCRKRARTPFEPVYADADATYAITADIDVTTFEPMVARPGEPH
                     DTVGVRELPVTRIDSAFIGSCTNGRMNDMRVTAEILKGRKVAPGVVLKIVPSTDAVWK
                     QCLDEGLMDIFKDAGALVSNAGCAGCAAGQVGQNGKGEVTVSTGNRNFPGKQGQGSVY
                     LASPAVVAASAVAGYITTPGMIPATPPEFKFSATEPPAPAAVRSAETDRPTVIRGRVW
                     LIERDNIDTDMIFHNRYLSITDIREMGQYAFDNLEGYKDFAKKAQAGDIIVAGKNFGS
                     GSSRQQAVDCFVSLGIHAVVAQSFGAIYERNAINAAFPILTYSSLEKIDLRDQDVISI
                     NMLTGETVNERNGLKTLINPFYDAQYEIYKRGGLLGKI"
     gene            complement(4066..>4741)
                     /locus_tag="PLA17_09470"
     CDS             complement(4066..>4741)
                     /locus_tag="PLA17_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPK85681.1"
                     /translation="DLVMYGPDLRHFWNFYRKDKNDTGRHAIMVHFRRDSLGESMLSQ
                     FEMADLRRLLENAGRGIAFSPETGEKAEEPMRRMLSDTGMKKMVSFFRLLEILCSAPK
                     FKILSGENLEGVREITDSRLTEVCSFIRENFYRPIPVKEIAVVAKMSPHSFSRFFSRS
                     TGSGFVNYVNQVRTNKACYLLRETDHQINEIVFECGFATISNFNKQFRKHAGMSARDY
                     RAQYRT"
BASE COUNT         1084 a         1265 c         1145 g         1247 t
ORIGIN      
        1 tgagtccgga aatcttaatt acggatctga aactgatggt gttcattcca ccttcatctg
       61 tcatgcccgg gcgttgcagc cccggctttc gtgattccgg agcgactcga acgctcgacc
      121 cacagcttag aaggctgttg ctctatccaa ctgagctacg gaaccgtacc gcggtttata
      181 aaaaaccgcg gcaaatataa gcagattttt ccgtttgtca accggtattt tttaccggta
      241 tggatatgaa agtcagaccg tctttatcca gttgacaagt cccttcttct ctatgattgc
      301 cttcagttca ggaggcagtg gttcaaatct gaactgtttc tctcctacct taagcattcc
      361 actgtcaaag tctatctcca ccgcatcacc tgcacggtac gcctccacgg cctccctgtg
      421 cactatcagc ggcaacccct ggttaattga ggaacggtag aagatacggt tgactgactt
      481 gatgatgaca gccctgaccc ccagatgggc aagccccacc gacggatgtt cccttgagct
      541 gccacacccg aagttgtcgc cggcaatgat gatgtcaccg gccttcgcat ttgaactgaa
      601 cgagggatca aagtcgccga acaacaacgg aagaattgca gccgcatcag aactcataac
      661 ggagtaagtg tgcttgccgg caaacatctg atcagtgttc aggttgtcaa catctgcata
      721 attccagaca ttgccttcct ttctgttctc tccctcgcta atggtgtttg tcaggctctg
      781 cggaggattg taaggaaaca gctcatcacc ctttgtaacc ctggggtcgg taattacccc
      841 ggtgatagct gatgcagcca ctgtggctgg tgaagcaaga taaatagaag cctccttgtt
      901 gcccatcctg cccaggaagt ttctgttggc tgtagagatg actgtatgac cgttggcagg
      961 tatcccctgc cctgtaccca ggcatgggcc gcaactggga ctgaggatgt tggcgccggc
     1021 ctctatcagg tcagtgacta taccctccct gatggcctga agataaatct ccttcgatgc
     1081 ggggatgata tgaagctgga agcccggttt cacctttgca cccctgagga ctgacgctgc
     1141 tgtccggaga tcctcaatcc tgccattggt gcaggtgccg atcagtcctt catgcacaac
     1201 ggtaccggcc accgaagcaa ccgactttat attgtcaaca tgatgaggag ctgccactag
     1261 cggaaatacc tccgacaggt caatggatat gctgcttttg tattcagcgc cctcgtcagc
     1321 ccagataccg ggagtgttgg aaccaatgaa cttctgcaac accttatcag ccggaaaaac
     1381 agcattcttg gctcccatct ccgatgcaag atttgcaaca gtcatcctct cgctgacgct
     1441 gagcgaagca actccctctc catgaaattc tatagacatg taattggcgc cgtcagagcc
     1501 cagcatccct atgatccaca gcgacaggtc ctttgcagtg acacccggac gcagccggcc
     1561 ggtcagctca attctcattg aaggaggaac aaggaaccac gtctcgcctc tcttccagat
     1621 tcctgctgac tcggtgcggt ctattcccgc tgcaagggca ttgaaggccc ctgccgtaca
     1681 ggtgtggctg tcagaaccga caataatcat tcccggacgg gcatgatttg acatcatctg
     1741 atggcagata cccatccctg agtcgtaaaa cttactgatt ccctgtcctg ccactatgtt
     1801 cctgatatcc tggtactgtg tagccagttt ggcatcagca ggcggggcat tgtgatcaag
     1861 aactatgacc atctggtcag gatctgacac cttctcccct cccattttct tgaatgtctg
     1921 gaagatactt gcggtgttgt catgtgtcag gatcagatcg ggtttcctga atactactgc
     1981 cccctccgga gcgcccagtg ctttttcaac aaatgtcctt ggtttcatta taatataact
     2041 ttgatattaa agatataatg gaacccgcat gaccgaaaat tatacaaacg ggtgtttgtc
     2101 tgtttgatac atgcgggttt tatctgatga tatctctttc gttaaatttt acccaataaa
     2161 ccaccccttt tataaatctc atactgagca tcatagaacg gattaattaa agtttttaat
     2221 ccgttgcgtt cattcactgt ttccccggtc agcatattga tggagatgac atcctggtcg
     2281 cggaggtcta tcttttcgag gctggagtat gtaagtatgg ggaatgcagc gttaatggca
     2341 ttgcgctcat agatggcgcc gaatgactgt gctacaacgg catgtattcc cagtgagaca
     2401 aagcagtcaa cagcctgctg gcgtgagctg ccgcttccga agtttttccc ggcaacaatg
     2461 atatcgcctg cctgggcctt ctttgcaaaa tccttgtaac cttccaggtt gtcaaatgca
     2521 tactggccca tctcgcgtat gtcagtgatg gagaggtacc ggttgtggaa gatcatgtca
     2581 gtgtcaatgt tgtcacgttc tatcagccat acccttcccc ggatgacggt gggtctgtca
     2641 gtttccgcgc tcctgacagc ggcaggagcg ggtggctctg ttgcgctgaa tttgaattca
     2701 ggcggggtgg ccggaatcat tccgggagtt gtaatgtatc ctgcaactgc tgatgctgcc
     2761 accacggccg gagatgcaag gtagacgctt ccctgcccct gtttgcccgg gaaattccgg
     2821 ttcccggtac tgacggtgac ctcacccttg ccgttctgac ctacctgtcc ggctgcacat
     2881 cctgcgcaac cggcatttga gaccagtgct ccagcatcct tgaagatatc cataagtcct
     2941 tcatcaagac actgtttcca taccgcatcc gttgatggca caatcttgag gaccacgcca
     3001 ggagccactt ttctgccctt cagtatctca gccgtcaccc tcatgtcatt catacggccg
     3061 ttggtacaac tgccgatgaa ggctgagtct atccttgtca cggggagttc tctgacccct
     3121 accgtgtcat gcggttcacc cggacgtgcc accatcggtt cgaaggtggt gacgtctata
     3181 tctgctgtta ttgcataggt agcatctgcg tcggcataga caggttcaaa aggtgtacgc
     3241 gcgcgcttgc ggcaatattc gacaatggcc ggtgttggcg ggaagagaat tattattgct
     3301 cccatctctg ttcccatgga ggctatggtg atccgttcgt cgagcgacat ctggtccact
     3361 gcatcaccat acagctcaat gctgtaaccc agcagggtat ttgcgccata gatacgcagc
     3421 aggttcagaa tgacatcctt ggcagtcacg ccatcaggca ttatgccctt cagtgtgagc
     3481 ttcaccgatg ccggaacctt gaaccataca cttccgctgt tccatgcagc ggcaatgtcc
     3541 atgtcaccca ttccctgacc aaatgcgccc acggcaccca gtatgtttgc atgtgaatcg
     3601 gttgacactg cagtagctcc cggccataca tagccctgct caatgagaag gtgtgtacct
     3661 atgcctgcat caatgtcata tacctttaat ccgttaccgc gggcatactg cctgcatata
     3721 tgctggttaa cggcgtattt ctggtctgaa cccgtaggat tgcagtcgaa cgtgaagaga
     3781 gtcctttcag ggtcatcaac cttcagtccg aaactgttca ggttcttgac gacattggcg
     3841 ccgccgaagt ctcttgctgc acgtgcgtca atagtaatgt taacaatatc acccggtttt
     3901 acgctatcgt attttgagtg agcggcaatg attttttcgg ttatggtcat tcccatgatt
     3961 gagttgtttt aagttggttc agaggcaaat ttagcaatag agatcgagct ccggcatgaa
     4021 taatatcatc agttgagagc tattttcctg cagaaaggca gtatgtcagg tccggtactg
     4081 agcacggtag tcacgtgctg acataccggc atgtttgcgg aactgcttgt tgaagttgga
     4141 gatggttgca aacccgcatt cgaagacaat ttcgtttatc tggtggtcgg tttcccggag
     4201 aagataacaa gccttgttgg tacgcacctg gttcacatag ttgacaaacc cgctgccggt
     4261 actgcgtgaa aagaagcgac tgaatgaatg ggggctcatc tttgccacca cagcgatctc
     4321 cttcaccggg atcgggcggt aaaaattctc tctgataaag gagcagactt cagtcaggcg
     4381 actgtcagta atctcacgca ctccttcgag gttttcgcct gaaagaatct tgaacttcgg
     4441 tgccgaacag agtatttcga gcagtctgaa gaagctgacc atctttttca tcccggtatc
     4501 ggagagcatt cgccgcattg gctcctctgc cttttcgccc gtctcgggcg agaaagcaat
     4561 cccccggccc gcattttcaa gcaaccgcct caggtcagcc atctcgaatt gggatagcat
     4621 cgactcacca agcgaatcac gcctgaagtg aaccattatt gcatgcctgc ctgtatcatt
     4681 tttatccttc ctgtagaagt tccagaaatg cctgaggtca ggaccgtaca tcaccaggtc
     4741 a
//
LOCUS       DAOPBK010000177         4721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15108_length_4721_cov_4.158594, whole genome shotgun sequence.
ACCESSION   DAOPBK010000177 DAOPBK010000000
VERSION     DAOPBK010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15108_length_4721_cov_4.158594"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1982)
                     /locus_tag="PLA17_09475"
     CDS             complement(234..1982)
                     /locus_tag="PLA17_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85682.1"
                     /translation="MPATLSPDFSDKKTLEKYSSAYTLSDMEIFIFPELFYPLVLANI
                     MSPEIWKWRNDPWFRDMHRKNFISKANRIKQYIIDNYIFNLDLETWGLTDKETELERF
                     SDFFDTKLLKQSNALFGYEGDKYYFSIDIRRHFGLDKYKSSVIPYWKTETVEAMAAFR
                     HREHYTTGAGECVSLATLYAAAMFIVGEVPLEKIFIMATPLHSQNFINEKEGLLTNNR
                     RIMTKNMWFNGTSLSEKARRALENEKVIIVSHITGHIHTVYKRATIDRDAYNTFAKKL
                     TSFVKSPLTPAIFINFLRFKSEYKCLFQYHYLRAGTSHYITLEKLFEYEHSLKVSMNE
                     ETRGRLLAEVDSEEFQYDPVPGKIILNEVEAFIKRHRDSDLKVIEAEFTESFPTRHTD
                     CVRKMFADIREFIITEPRLPSADREFEPETHPQISVTDSRDEIISKIRSLAGVSEMAL
                     LTLYVYRDMALTDWKPFVKAAIERNPVCHADLGGRDAEEIYSLINELTNESIYGPGRL
                     AQPDEVWNFRRGDGAEKAFLMADALVFNDPGAEVEIIVEDDQAVVGYNHRHFRFATVK
                     GLRKKITIRGKEYIIS"
     gene            complement(2025..2444)
                     /gene="ndk"
                     /locus_tag="PLA17_09480"
     CDS             complement(2025..2444)
                     /gene="ndk"
                     /locus_tag="PLA17_09480"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPK85683.1"
                     /translation="MSNDITFSIIKPDALGSGNAGSIISRINKAGFRIAALKMLHMTR
                     AQAESFYEVHRERPFYPELVEFMSSGPALVMVLRHTDAVEEFRRLIGATDPARALPGT
                     IRADFGTNVRMNAVHGSDSPENALREASFFFSAIEIY"
     gene            2491..3063
                     /locus_tag="PLA17_09485"
     CDS             2491..3063
                     /locus_tag="PLA17_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPK85684.1"
                     /translation="MPEFGKYTKELKKLIDEADHILLICHINPDGDAVGAMLALRRWI
                     KSRGRDAAMISPNALQKFLLWMNDVDKIVDFHHNPDEGTALINNADLIIMVDFNSSSR
                     MGKAEQLVISSGARKVMIDHHPDPAPLADLTISDVSRSSTSELVYFLVNGMEGCEFND
                     SEFITAVYVGIITDTGNFEHGYYTHGWSPA"
     gene            3023..3517
                     /locus_tag="PLA17_09490"
     CDS             3023..3517
                     /locus_tag="PLA17_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPK85685.1"
                     /translation="MSTGTIRTVGHLLDMGVDKENVYNHIFSNFSADRIRLKGFALHS
                     RMVVHPDLHTAYIWLTKEDLDRFHYVKGDTEGFANVLLTMNGIVLSVLFVERTGFVKM
                     SLRSKGSFNANDLSRKCFNGGGHRNAAGGEMKGTIHEVVNHFENTLPSLRKELEEAAR
                     KIAK"
     gene            3514..4017
                     /locus_tag="PLA17_09495"
     CDS             3514..4017
                     /locus_tag="PLA17_09495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPK85686.1"
                     /translation="MRLTAYIVMIAVIAAGCQNKPDSRSKASPPTNAELIELNRSLIT
                     QDRNTINEWVSKSDRNFTETSTGLWYSILGHGSGPTIKTGDNVNFDYECTLLNGNPCY
                     NGNQTIRVGYTDAGSGVTEGLQMMQEGSDYLFIVPPYLAFGLTGDGAKIPGRAILIYR
                     IRVRDIN"
     gene            4061..4564
                     /locus_tag="PLA17_09500"
     CDS             4061..4564
                     /locus_tag="PLA17_09500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPK85687.1"
                     /translation="MGKKINAAALAILAVIIVACNPSKKYEEEERSQILDYVAANNIT
                     VSPDANGIYYIETVAGTGELIRKGDSVGVWYTGSFLNGNKFDDNIEAETPFRFRVGSP
                     YIIDGWSLTLVKMKMGSKANVIMPSKVAYGSIGVRAYDGYGILRTIIPGYTPLLFEME
                     VVELVRE"
BASE COUNT         1224 a         1146 c         1174 g         1177 t
ORIGIN      
        1 gtgattcgct gaagagccgg ataaacagca gggctggaga ggaagtggtc agggaaataa
       61 tgctgcattg agagagccgg gcagcatgac tccgcagcat gaatcagctt caagactctg
      121 catcaagact cctcagcatg actgctcatc atgaatcttc agcatgaacc cgaatcatga
      181 ctccgtccgg taacagatgt ctcttccatg cgtgaccgaa agggtcatcc atctcacgat
      241 ataatgtatt ccttcccgcg tatggttatc tttttcctga gccccttgac tgttgcaaac
      301 cggaaatgcc tgtgattgta tccgaccact gcctggtcgt cttccactat tatttccacc
      361 tcggcgccgg gatcattgaa gaccagggca tcagccatca ggaaagcctt ttccgcgccg
      421 tcacccctcc tgaaattcca tacctcgtca ggctgtgcca gtctccccgg gccgtatatc
      481 gattcgttgg tcagttcatt gataagtgaa tatatctctt cggcatcccg ccctcccaga
      541 tctgcatgac ataccgggtt cctctcaatg gcagccttga caaacggctt ccagtcagtg
      601 agtgccatat ccctgtagac ataaagtgtc agcagtgcca tctcggatac tccggcaaga
      661 ctcctgatct tgctgattat ctcgtcacgg ctatcagtta ctgatatctg cggatgggtc
      721 tccggctcaa attccctgtc agccgagggg agcctcggct cggtaatgat gaactccctg
      781 atgtcagcaa acatcttcct tacacaatca gtatgcctgg ttgggaaact ctctgtgaac
      841 tcagcctcaa tgaccttcag gtcagagtct ctgtgccgtt taatgaatgc ctctacttca
      901 ttcagaataa tcttgccggg aaccgggtca tactggaact cctcactgtc tacttcggca
      961 agcagcctgc cacgggtctc ctcgttcatg cttactttca gcgagtgctc atactcgaac
     1021 agtttttcaa gggtgatgta atggctcgta cccgcccgga ggtaatgata ctgaaagagg
     1081 catttgtatt ctgatttgaa cctgaggaag ttgatgaaga tggcaggggt aaggggcgac
     1141 tttacgaagc tggtcagctt tttggcgaag gtgttatatg catccctgtc gatggttgcc
     1201 ctcttgtaca cggtatggat atggccggtg atatgagaaa ctattatcac cttttcattt
     1261 tccagggctc ttctggcctt ttctgacagt gaggtgccgt tgaaccacat gtttttggtc
     1321 ataatcctcc tgttgtttgt cagcagcccc tccttctcat tgatgaaatt ctgcgagtga
     1381 agcggcgtgg ccattatgaa tatcttttca aggggaacct cgcctacaat gaacatggct
     1441 gcggcataga gcgttgccag gcttacacac tcgccggcgc cagtggtgta gtgttcacga
     1501 tgcctgaatg ctgccatcgc ctcaactgtt tcagtcttcc agtagggtat gacagatgat
     1561 ttgtacttgt cgagcccgaa atggcgacgg atgtcaatac tgaaataata tttgtcacct
     1621 tcgtaaccga aaagtgcatt tgattgctta agcagtttgg tatcaaagaa atcactgaaa
     1681 cgctccagtt ctgtttcctt gtctgtcagg ccccaggtct cgagatcaag gttaaatata
     1741 taattgtcaa tgatgtactg tttgatcctg ttggccttac tgatgaagtt cttacggtgc
     1801 atatccctga accacgggtc attgcgccac ttccatattt ccggtgacat tatattggcc
     1861 agaaccaggg ggtaaaacaa ttccgggaaa ataaaaatct ccatgtcgga gagggtatag
     1921 gcagaagagt acttttcgag ggttttcttg tccgaaaagt caggagataa agtagcaggc
     1981 atttcagttt tttatctaaa caagcaggca ggattattgt ttactcagta tatctcgatg
     2041 gcagaaaaga agaaggaggc ttccctgagt gcattctccg gggagtcgga gccatgaaca
     2101 gcgttcattc tgacatttgt gccgaagtct gcccggatgg ttccggggag cgccctggcc
     2161 gggtcagtgg cacctatcag cctgcgaaac tcttcaacgg catcagtatg cctgagtacc
     2221 atcaccaggg ccggacctga tgacatgaac tcaaccagct ccgggtaaaa gggcctctcg
     2281 cggtgaacct cataaaaact ttctgcctgc gccctggtca tgtgcagcat cttaagcgca
     2341 gcaatcctga atcctgcctt gttaatacgt gaaataatgc ttcccgcgtt gccgcttcca
     2401 agcgcatcgg gcttgatgat tgaaaacgta atatcgttgc tcataactct atttttcttc
     2461 ttacctttgt cgccttaaaa gataaaagca gtgccggagt ttggtaaata tacaaaagaa
     2521 ttaaagaagc tgatcgatga agccgatcat attctgctca tctgccacat caatcccgac
     2581 ggcgatgccg taggggccat gctggcgctg agaaggtgga tcaaatcacg gggcagggac
     2641 gctgcaatga tctcacccaa tgccctgcag aagttcctcc tgtggatgaa tgatgttgac
     2701 aaaattgtcg attttcatca taaccctgat gagggtacag cattgataaa caatgccgat
     2761 cttatcatca tggtcgattt caacagctcc tcaaggatgg gaaaggcaga acagctggtt
     2821 atcagttcag gcgccaggaa ggtcatgatt gaccatcacc ccgaccccgc gcctttggct
     2881 gatcttacca tctctgatgt gagcagaagc tccacctcag aactggttta cttcctggtg
     2941 aacggaatgg aaggctgtga gttcaatgac agtgaattca tcactgcggt atatgtcggt
     3001 atcatcactg atacgggcaa ttttgagcac gggtactata cgcacggttg gtcacctgct
     3061 tgatatggga gtagacaagg agaatgttta taaccatata ttcagcaact tctcggctga
     3121 caggataagg ctcaagggat tcgcgctgca cagccgcatg gtggtccatc ccgatctgca
     3181 tacagcatat atctggctca caaaggaaga tcttgacaga ttccactacg tcaagggtga
     3241 caccgaagga tttgcaaatg ttctcctgac tatgaacgga atagttctct ctgttctctt
     3301 cgtggagcgt accggcttcg tgaagatgtc gcttcgctcg aagggcagct tcaacgcgaa
     3361 cgatctgtca cgaaaatgct ttaatggcgg aggacacagg aatgccgcag gaggggagat
     3421 gaagggaaca attcatgagg tggtgaatca tttcgaaaac actcttcctt cgctccggaa
     3481 ggaactggag gaggctgcca gaaagatagc gaaatgaggc tgacagcata catcgtcatg
     3541 attgctgtta tagcagcagg ctgccagaac aagcctgaca gcaggagcaa agcctcaccg
     3601 cccaccaatg ccgaactcat cgaactgaac aggtcgctca taacacagga ccggaatacc
     3661 atcaatgaat gggtcagcaa aagcgaccgc aactttaccg aaacaagcac cggtttatgg
     3721 tacagcatcc tgggccacgg ctccggcccg acaatcaaaa cgggagataa tgtaaacttc
     3781 gattacgaat gcactctcct gaatggcaat ccctgctaca acggcaatca gacaatcagg
     3841 gtgggatata ccgatgccgg aagcggtgtc acggaaggac tgcaaatgat gcaggaaggg
     3901 agtgattacc tgtttattgt accaccctac ctggcgttcg gactgaccgg cgacggcgca
     3961 aaaatacccg gaagggcaat acttatctac aggataagag taagggatat aaactagttt
     4021 ggaacgatat ttgacgtgaa ttgtggaaac taatagtact atgggaaaga aaatcaatgc
     4081 tgcagcactg gccatactgg ccgtaattat cgttgcctgc aatccttcaa agaaatatga
     4141 agaggaagag agaagccaga tactggacta cgttgctgcc aataatatca ccgtaagccc
     4201 tgacgcaaat ggcatctatt atatcgaaac tgtggccggc accggtgaac tgatccggaa
     4261 gggtgactcg gtgggggtat ggtacacggg cagtttcctg aacggaaata aattcgatga
     4321 taacatcgag gcagagacac cattcaggtt cagggtgggc tcgccatata tcattgatgg
     4381 atggtcactt accctggtca agatgaagat gggatcgaaa gccaacgtaa tcatgccctc
     4441 aaaagtggca tatggatcaa ttggcgtaag agcatatgac ggttacggta tcctgcgcac
     4501 gataattccg ggctatacac ctttgctctt cgagatggag gtggttgaac tggtcaggga
     4561 ataaaatgaa tgcgccgccc tcctgaagcg gcgcattttt atttcagtac aacagcatca
     4621 ccggaaaggg tcacctgact gagcttctca acccaacccg cccaggtgcc ggccatgagg
     4681 gcatccgcaa gcttcagccc ttcatcaagg gctgcttcag c
//
LOCUS       DAOPBK010000178         4680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15262_length_4680_cov_6.182054, whole genome shotgun sequence.
ACCESSION   DAOPBK010000178 DAOPBK010000000
VERSION     DAOPBK010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15262_length_4680_cov_6.182054"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="PLA17_09505"
     CDS             <1..1430
                     /locus_tag="PLA17_09505"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK85688.1"
                     /translation="IVEENILTATEVKVETGVAEEIKKTPTSFDRSVKPPLGPDVSVT
                     IPAVWKSSLENGMKLYGIQHTEVPLVQYEIVIEGGHMLDDISAPGVASMLATMLNEGT
                     RNKTPEELEEEIDLLGASIRIGGGSENITVSVNTLARNFEKTLAIVEEMLLQPRWDAT
                     AFELAKTRTLNSLRRSSIDPTYLGSNALKNLIMGENNIYATDVSGTTESVQAMTMDQL
                     KAFYEKNLSPSISRFLVVGAVDQPRVEKALASLNANWAAKEVQMPEVLPAQTPEKSGV
                     WFVNIPDAKQSNIYIGAPIIPRGHPDYYPLYVANYKLGGSFSGYVNLVLREEKGYTYG
                     ARTNLIAMKNYGYFSTTAAVRSTATLESVEIFRDLMARYREGVSQEDVDFTKDALLKG
                     NALRFETQRALLGVISTMSEYGLPDDYIAQEENYVRELTVEKVNEMVNKYIDPMKMYY
                     VVAGDAATQLKDLKKLGFGEPVLVK"
     gene            1524..4328
                     /locus_tag="PLA17_09510"
     CDS             1524..4328
                     /locus_tag="PLA17_09510"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK85689.1"
                     /translation="MMKRTISFFMIASMLLMTSCGGKEKNRLSIPYEEYKLDNGLTVI
                     LNEDKSDPIASLVILYHVGSSREVPGKTGFAHLFEHMMFQRSENVGEDEYFKYIEGAG
                     GNLNGGTSYDQTIYFEVVPKNALELAMWLESDRMGFLRNTVTPQSFALQQNVVQNEKR
                     QGVDNRPYGHTEAVILKNLYPQDHPYSWDVIGEMADLANATIDDVKDFHKKFYIPNNA
                     TLVVSGDYDVAEVKAMIEKYFGEIPAGEELKDPDPMPVTLSETKKIYHEDNFARAPQY
                     TMVFPTVEQFGKDAYSLDVLAQLLGVGKKSPMYKVLVKEKQLTSNVSVYNQALELTGR
                     FQIGVTANPSTSLKEVEEAIFEAFTRFETEKFTEQDLERIKAGLETDFYRMMSSVMYK
                     SFMLAMYSEFAGSPDFMVEELEMMKKVSMDDVWDVYNRYIKDKNYIATSFVPKGQTDL
                     IAEGSVIADIVEEDVTRATQMEVIAGGEEVIEKTPSSFDRSKPPVPGPDPSVTLPSIW
                     KSEAANGMKVFGIEQHELPLMTYNIVITGGHMLDDISKPGVARFTAQMLNEGTKNKTP
                     EELEEAIQLLGASISIRASEERITVSVSALARNFEKTLALVEEMLLEPRWDEEQFSIN
                     KTRTINNLKRNLADPNYVAGITFGKLVLGADNIHSTDVSGTVESVETITMDDLKAYYE
                     KNFSPSIATFLIVGDVDQARAEKALAGLNEKWAAKEVTMPELTFPPAPEKSAIYFVDI
                     PGAKQSVINIGTPSIHRDHPDFYKADVANFMLGGGASARLFMVLREEKGFTYGAYSGF
                     DGMKNYGTFTAYASVRTDATLESVELFRDIMAQYRAGVSQEVVDFTKGSLLKGNALRF
                     ETNEAKLGMLNTMTFYGLPDDYVKQEEDYLRGLTVEQVNETVQKYIDPMKMYYVVVGD
                     GATQFKGLKKVGFGEPVLVK"
     gene            4595..4679
                     /locus_tag="PLA17_09515"
     tRNA            4595..4679
                     /locus_tag="PLA17_09515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4628..4630,aa:Leu,seq:taa)
BASE COUNT         1266 a         1076 c         1252 g         1086 t
ORIGIN      
        1 gcattgtgga ggagaacata ctgactgcca cagaggtaaa ggtggaaacc ggtgttgccg
       61 aagagattaa gaagaccccg acttcttttg accgttcggt taagccaccg ctcggccctg
      121 atgtatctgt tactattccg gcagtatgga agtcatcatt ggagaacggc atgaaacttt
      181 acggtataca gcacacggaa gtacctcttg tacagtacga aattgtcatc gagggaggtc
      241 acatgcttga tgatataagt gctccgggtg ttgccagcat gctggcaacc atgctcaatg
      301 agggtaccag gaacaaaacc cctgaggagc ttgaggaaga gattgatctc ctgggagcca
      361 gcatccggat cggaggaggc agtgaaaata ttaccgtgag cgtaaacaca cttgcccgca
      421 attttgaaaa gacccttgcc attgttgagg agatgttgct acagccccgg tgggacgcta
      481 ccgcctttga actggccaaa acccgcactc tcaacagcct caggaggagc tctattgatc
      541 ctacatatct gggctcaaac gccctcaaga atctgatcat gggtgagaat aacatttacg
      601 ccaccgatgt gtcaggcact acagagagcg tacaggcgat gaccatggat cagctcaagg
      661 cattctatga gaagaatctc tctccttcaa tctcgcgctt cctggttgtg ggagccgttg
      721 accagccaag ggttgagaaa gcgcttgcat cgctgaacgc caactgggct gccaaagagg
      781 ttcagatgcc tgaggtcttg cccgcacaga cacctgaaaa gtccggcgtc tggttcgtga
      841 acattcctga tgcaaaacag tcaaatattt atatcggagc gcctattata ccaaggggtc
      901 atcctgacta ctacccttta tatgttgcaa actacaagct cggaggttca ttcagcggtt
      961 acgtcaacct ggtcctcagg gaggaaaagg gttacaccta cggagccaga accaatctca
     1021 tcgcaatgaa gaattatggt tatttcagta ccaccgcagc agtaagatca actgcaaccc
     1081 ttgaatcagt ggagattttc agggacctga tggcgcgtta ccgcgaaggt gtctcacagg
     1141 aggatgttga cttcacgaag gatgcccttc tcaaggggaa tgccctcagg tttgagaccc
     1201 agcgggctct gctcggggtg atcagcacca tgagcgaata cgggcttcct gatgactaca
     1261 tcgcacagga ggagaactat gtaagggagc ttaccgtgga aaaggtaaac gaaatggtaa
     1321 acaagtatat cgatccgatg aagatgtact atgttgttgc cggagatgca gcaacacagc
     1381 ttaaggacct taaaaaactc ggcttcggag agcctgtact ggttaaataa aaaaacatga
     1441 aacccacatg tgtaaaatgg ataaacaggt gtttatctga catgtgggtt tctttatacc
     1501 tttaaagtca aaattattta tatatgatga aaagaaccat atcgtttttt atgattgcct
     1561 caatgctgct gatgacatca tgtggcggga aggaaaaaaa caggctgagt attccctatg
     1621 aagaatacaa gcttgacaac ggtctgactg tcatactgaa tgaggataag tcggatccta
     1681 tcgcttcact ggttatcctc tatcatgtgg ggtcaagcag ggaggtgccc ggtaaaaccg
     1741 gtttcgctca cctttttgag catatgatgt tccagcgttc ggagaacgtg ggtgaggacg
     1801 aatatttcaa gtacatcgaa ggtgcagggg gtaacctcaa cggaggcacc agctatgacc
     1861 agacaatcta cttcgaggtt gtgccgaaga atgcgcttga acttgccatg tggctcgagt
     1921 cagaccgcat gggattcctt cgcaacactg ttacgccgca gtcatttgcc ctgcagcaga
     1981 atgtggtgca gaacgagaag cgccagggtg ttgacaacag accctatggc catactgaag
     2041 ccgttatcct caaaaacctt tacccgcagg atcaccccta cagctgggat gtgatagggg
     2101 agatggctga cctggccaat gccacaatag acgatgtaaa ggatttccac aagaagttct
     2161 atatcccgaa caatgccaca ctggtcgtgt caggtgatta tgacgtggca gaggtgaagg
     2221 caatgatcga gaaatatttc ggcgagatcc ccgcagggga agagcttaag gatcctgatc
     2281 cgatgccggt gaccctgtca gagacgaaga agatctacca cgaagacaac ttcgcgcgtg
     2341 cgccgcagta cacgatggtg ttcccgaccg tcgagcagtt tggaaaggat gcctattctc
     2401 ttgatgtact ggcacaactg cttggtgtgg gcaaaaaatc accgatgtat aaggtgcttg
     2461 taaaggaaaa gcagcttaca tccaacgtat ccgtttacaa ccaggcgctt gagctcaccg
     2521 gcagatttca gattggcgtt actgccaacc cgtcaaccag cctgaaagag gtggaagagg
     2581 cgatttttga ggccttcacc aggtttgaaa cagagaaatt caccgagcag gacctggaga
     2641 gaatcaaggc aggccttgag actgacttct accgcatgat gagcagcgtg atgtacaaat
     2701 cgttcatgct tgccatgtac agtgagtttg caggctcacc cgacttcatg gttgaggagc
     2761 ttgaaatgat gaagaaggtg tcgatggatg atgtatggga tgtgtacaac cgctatatca
     2821 aagacaagaa ctacattgcc accagcttcg tcccgaaggg acagactgat ctgattgcag
     2881 agggttcagt gattgcagac attgttgagg aggatgtcac cagggcaacg cagatggaag
     2941 ttatcgccgg aggcgaagag gtgatagaaa agaccccgag cagttttgac aggtcaaagc
     3001 caccggttcc gggtcctgat ccgtcagtca ccctgccctc catctggaag agtgaagcgg
     3061 ccaatggtat gaaggtcttt ggtattgagc agcatgagtt gccgctgatg acatacaata
     3121 ttgtcatcac gggcggtcac atgctcgatg atatctccaa accgggtgtt gccaggttca
     3181 ctgcccagat gctgaatgag ggaacgaaaa acaagacccc ggaagagctt gaggaggcga
     3241 tacagcttct tggagccagc ataagcatca gggccagtga ggagagaatc accgtttctg
     3301 tgagcgcact tgcccgcaac tttgagaaga cccttgcact tgttgaggag atgcttcttg
     3361 aaccccgctg ggatgaggag cagttctcaa tcaacaagac ccgtaccatc aacaatctaa
     3421 agcgcaatct tgctgatccg aactatgttg ctggcattac tttcggcaaa cttgtgctgg
     3481 gtgctgacaa tattcattca acggatgtca gcggtacggt ggagagcgtg gagacaatca
     3541 cgatggatga tctcaaggct tattacgaaa agaacttctc accttcaatt gccaccttcc
     3601 tgattgtggg tgatgtagac caggccaggg cagagaaggc acttgccgga ctgaatgaga
     3661 agtgggctgc aaaagaggtg acaatgccgg aactcacatt cccgcctgca ccggaaaaat
     3721 ctgcaatcta ttttgtggat attcccggag ccaagcagtc tgtcataaac ataggcactc
     3781 cctcaataca ccgcgaccac cctgatttct acaaggctga tgttgccaat ttcatgcttg
     3841 gcggaggtgc cagtgcaaga ttgttcatgg ttctcagaga ggagaagggg ttcacctatg
     3901 gcgcttactc tggtttcgac ggcatgaaaa actatggtac attcacagcc tatgcttctg
     3961 tgcgtaccga tgccaccctg gagtcagtgg agctgttcag ggatatcatg gcacagtaca
     4021 gggcaggtgt ctcgcaggag gttgtggact tcacaaaggg atccctgctc aagggcaatg
     4081 ccctcaggtt tgagaccaat gaagcgaagc tgggcatgct gaacacaatg accttctacg
     4141 gtcttcctga tgactatgtg aagcaggagg aggattatct ccgcggtctg accgttgagc
     4201 aggtcaacga gacggttcag aagtacattg acccgatgaa gatgtactat gttgttgtcg
     4261 gagacggtgc cacgcagttc aagggactga agaaagttgg cttcggcgaa ccggttcttg
     4321 tgaaatagag attgtaagca ggttgaaaca cagagttccg ggcttgcgca ctcttgcgaa
     4381 tccggaactc tttttttgat taatcactaa gcccgggaaa taaaactgac tgccataaat
     4441 ttgatttaca tatcctatat ttgcacaacc atctgagaga tcgtggtccg gctggtttca
     4501 ggaccttcag actgagctac gtctgtgacg gagcgaaatc acgataacga agctgtcggg
     4561 gttttcgggc tctcagacct tcatatttcg aaccgcccgg atggtggaat ggtagacacg
     4621 ctggacttaa aatccagtgg gcagtaatgc ctgtgcgggt tcaagtcccg ctccgggtac
//
LOCUS       DAOPBK010000179         4633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15441_length_4633_cov_4.084098, whole genome shotgun sequence.
ACCESSION   DAOPBK010000179 DAOPBK010000000
VERSION     DAOPBK010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15441_length_4633_cov_4.084098"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLA17_09520"
     CDS             complement(<1..759)
                     /locus_tag="PLA17_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HPK85690.1"
                     /translation="MRKRRGRTQDDVASALQMKRPTLSGYENGVAQPGIEALVAFSGY
                     FNISLDTMLKVDLTRLSESQLGELERGYDAYIRGSNLRVLATTVTPDNRENIELVSEK
                     AKAGYTTGYADPEFIGDLPLFNLPFLSPDRKYRTFQLKGDSMLPIPDRSWVTGEFMQD
                     WRDIKSGQAYIILTLDDGIVFKVAENNIEKSGRLVLYSLNPLYEPYEVHISDIREVWK
                     FVHYISDELPEPVLPEKQLLRTVASMKQDLYQLKK"
     gene            998..2242
                     /gene="dinB"
                     /locus_tag="PLA17_09525"
     CDS             998..2242
                     /gene="dinB"
                     /locus_tag="PLA17_09525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPK85691.1"
                     /translation="METRSILHLDLDTFFVSVERVRDSRLNGRPVIIGGSSDRGVVSS
                     CSYEARSFGVRSAMPMKMARAMCPEAVFIRGDMELYTKYSRMVTDIIADKAPLYEKAS
                     VDEHYVDLTGMERFHGCASWSHELRDTIIRETGLPISIGLSVNKTVSKIAAGEAKPNG
                     EREVAQQQVLPFLDPLSIRKIPMIGRKTYHTLRSMGISTIHTLRVIPAEMLGSLMGQN
                     GMEIWKKANGIDNTPVIRYSEQKSVSTEHTFDRDTTDTVMLNQMIVSMTEKLAYELRR
                     QEKLASCLTVRIRYSDFDTHTLQQRVPYTAFDHLLISEAQNLFRRLWQRRMLIRLIGV
                     KVSGLVRGVQQLNMFEDTPEMVNLYMTMDRLRGRFGRHAIRRAAGVMTAAERSERELR
                     KASELLAEKSRIEERLRGWHFH"
     gene            2277..>4633
                     /locus_tag="PLA17_09530"
     CDS             2277..>4633
                     /locus_tag="PLA17_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPK85692.1"
                     /translation="MYINCHTYNSLRYGTLTVERLSELAAAAGVERLVLTDINNTTGI
                     PDFVRECRSRDITPAAGIEFRDGNRLLYIGIARNNEGFRELNEFLTHHNVTGESLPPR
                     PPSFPNAYVIYPADGGNSTGGKRNSTSSGGSSTAGAIIPLAEMNGSNSAGMNGSAVTG
                     RVTDNIIVSPALRDNEYIGIRPSAASRLISMKKSDIARCVILHTVTLESPADYPVHRS
                     LRAVDNNILLSRLTPEMTCSPDEYIIPEADAAGPFSMWPQVTGNTRRLLNDCSLELDF
                     TTPKNKRIYSASRYDDKLLLEKLAWDGMKYRYGKNNKVAEERVRHELEIIDRLGFSAY
                     FLITWDIIRYSMACGFYHVGRGSGANSVVAYCLKITNVDPIDLNLYFERFINPKRSSP
                     PDFDIDYSWKERDAVTEYIFRRYGHRHTALLGTINTFRGKSIVRELGKVHGLPADEID
                     ALIANPSAESNRGEIQDLIFETGNKIADFPNLRSIHAGGVLISEDPLSCYTALDMPPK
                     GFPTTQWDMYTAEELGYEKLDILSQRGIGHIRESAEIIRANRGVDVDIHAIQRFKNDP
                     LVKDHLRRGDTKGCFYIESPSMRGLLQKLKCSDYLTLVAASSVIRPGVARSGMMRQFI
                     YRFHHPDNFEYIHPVMKDLLSETFGVMVYQEDVLKVCHHFAGLDLSDADTLRRAMSGK
                     YRSRREMQKITERFFSNCRDKGYPGEITKEVWRQIESFAGYSFSKAHSASYAVESFQS
                     LWLKAHYPLEFMVAVINNFGGFYRTWVYVQEARREGAVIEAPCINR"
BASE COUNT         1101 a         1268 c         1288 g          976 t
ORIGIN      
        1 ctttttcagc tggtacaggt cctgtttcat tgaagcaacg gtccggagga gttgtttctc
       61 ggggagtacc ggttccggga gctcgtcgct tatataatgc acgaacttcc atacttcccg
      121 gatgtcactt atgtggacct cataaggttc atagagcggg ttgagtgaat agagcacgag
      181 gcggccgctc ttctcaatgt tattctctgc aaccttgaag acgatgccgt catccaacgt
      241 aagaatgata tatgcctgtc cgctctttat atcacgccag tcctgcataa actcgccggt
      301 aacccatgat ctgtcaggga tgggcagcat ggagtcgccc ttcagctgga atgtgcggta
      361 cttgcggtct ggcgacagga atggcaggtt aaacagaggg aggtcgccaa tgaattccgg
      421 gtcggcataa ccggtggtat agccggcctt ggccttttcg ctcaccagtt caatgttctc
      481 acggttgtca ggagtgacag tggtggccag caccctgagg ttgctccccc tgatatatgc
      541 atcatatccc ctctcaagct ctccgagctg gctctcggag aggcgggtca ggtctacctt
      601 aagcatggtg tcaagtgaga tgttgaagta tccggaaaaa gccaccagcg cctctattcc
      661 gggctgtgcc acgccgttct catagccgct gagggttggc cgtttcatct gcagggctga
      721 tgccacgtca tcctgcgtac gcccgcggcg ttttctcaaa aattttatgt tcgatgcgaa
      781 gtacatatta taatatttga ttatattgta atcataagtt tgattaattt ctaatcaaag
      841 atagatgaag tttttgaaat agcaagcaga aaatgaaaaa aagaggcaag agggggccgg
      901 gcgtaaaaaa atgatccggc ggattatttt agcgaaggag ccggattgca gtgaggatag
      961 tagtggattc ccccgtcgag agccataaac gtaacaaatg gagacacgca gtatattgca
     1021 tcttgacctc gacacctttt ttgtatcagt ggagagggtt cgcgacagcc ggctcaacgg
     1081 ccgtcccgtg attatcggag gcagctctga ccgcggggtg gtctcgagct gcagctacga
     1141 ggcacgcagc ttcggcgtca gatcagccat gccgatgaag atggcccgtg ccatgtgtcc
     1201 cgaggcggta ttcatacgcg gtgacatgga gctgtacaca aaatattcaa ggatggtgac
     1261 tgacatcatc gccgataagg cgccgctcta tgaaaaggca tcggtggatg agcactacgt
     1321 tgatctcacc ggcatggagc gattccacgg ctgtgccagc tggtcgcatg agctgcgtga
     1381 cactatcata cgggagacgg ggctgcccat ctccatcggg ttgtcggtca acaagaccgt
     1441 ctcgaagatt gctgccggag aggcgaagcc caacggcgag cgggaggtag cacagcagca
     1501 ggtcctcccc ttcctcgatc cgctctcaat caggaagata cctatgatag gccggaagac
     1561 atatcacacg ctgcgctcta tgggtatctc cacgatacat acgctgcgtg tcatcccggc
     1621 tgagatgctc gggtcgctca tgggccaaaa cggcatggag atatggaaga aggccaatgg
     1681 cattgacaac acgcctgtca tcaggtactc agagcagaag tctgtcagca ctgagcacac
     1741 gtttgaccgt gacaccactg acacggtgat gctcaaccag atgatagtga gcatgaccga
     1801 gaagctggcc tatgagctgc gccgtcagga gaagctggca tcatgtctca cggtgcgcat
     1861 acgctactct gattttgaca ctcacacgct gcagcagcgg gtgccctaca ccgcctttga
     1921 tcatctgctc atcagcgagg cgcagaatct cttccggcgt ctctggcagc ggcgcatgct
     1981 catcagactc atcggggtga aggtgagcgg actggtgcgg ggtgtgcagc aactgaatat
     2041 gtttgaggac acacccgaaa tggtgaatct atatatgaca atggacaggc tgcgggggcg
     2101 tttcggccgt catgccatcc gccgggcggc aggggtgatg accgccgcgg agcgaagcga
     2161 gcgcgagctc agaaaggcgt cggaactgct ggcagagaaa tcacggatag aagaacggct
     2221 gcggggatgg cactttcatt agccgggaga gataggggca tccaaagctt taagtaatgt
     2281 acatcaactg tcatacatat aacagtttac gttacggtac actcaccgtg gagcgccttt
     2341 ctgagctcgc cgcggctgcg ggtgtggaac gactggtact gactgatatc aacaacacaa
     2401 ccgggatacc cgattttgta cgtgagtgcc gcagccgcga catcacccct gccgccggga
     2461 tagagttccg tgacggcaac aggctgcttt acattgggat agcacgaaac aacgagggct
     2521 tccgcgagct gaacgagttc ctcacgcacc ataacgtcac cggcgagtct ctcccgccgc
     2581 gccctccctc cttccccaac gcgtacgtca tctacccggc cgacggcgga aacagcactg
     2641 gcggaaaaag gaacagcacc agcagtggag gaagcagcac cgccggcgcc atcatacccc
     2701 tggcagagat gaacggaagc aacagtgccg gcatgaatgg cagcgccgtg acaggccggg
     2761 taacagataa catcattgtc tcgccagcac tgcgcgacaa tgagtacatc ggcatacgcc
     2821 cctccgccgc cagccgcctt atctcaatga aaaagagcga tatagcccgg tgcgtgatcc
     2881 ttcataccgt caccctggag tcgcccgccg actatccggt gcaccgcagc ctgcgcgcag
     2941 tagacaacaa catcctcctc agtcgtctca ccccggagat gacctgctca cccgatgagt
     3001 acatcatccc ggaagcagac gcggcaggcc ccttctccat gtggccgcag gtaaccggca
     3061 acacacgcag gctgctgaac gactgctcgc tggagcttga cttcaccacc ccgaaaaaca
     3121 agagaatata ctctgccagc aggtacgacg acaagctgct gctcgagaaa ctggcatggg
     3181 acggcatgaa gtaccgctac gggaagaaca acaaggtggc agaagaacgg gtgcgccacg
     3241 aactggagat aattgaccgc ctcggcttct cggcttactt cctcataaca tgggatatca
     3301 tacgctactc aatggcctgt ggattttacc atgtgggacg cggcagcgga gctaactccg
     3361 ttgttgctta ctgtttgaaa atcactaatg ttgatcccat tgatctgaac ctttacttcg
     3421 agcggttcat caatccgaaa cgaagctcgc cgcccgactt tgacatagac tactcctgga
     3481 aagagcgcga cgccgtaacc gagtacatct tccgccgcta cggccaccgt cacacggccc
     3541 tccttggcac catcaacact ttccggggca agtcgatagt ccgcgagctg ggcaaggtgc
     3601 acggactgcc cgccgatgag attgatgccc tgatagcaaa cccctccgct gagagcaaca
     3661 gaggtgaaat tcaggacctt atcttcgaga ccggcaacaa gatcgccgac tttcccaacc
     3721 tgcggagcat tcatgccggc ggggtactca tctcggagga tccgctaagc tgctacactg
     3781 cacttgacat gccgccaaag ggttttccca ccacccagtg ggacatgtac accgccgaag
     3841 agcttggcta tgagaaactt gatatactga gccagagagg cataggacat atcagggaga
     3901 gtgcggagat catccgtgcc aatcgtggtg tggatgtaga catacatgcc atccagcggt
     3961 tcaagaatga cccgctggtg aaggaccatc ttcgccgcgg cgataccaaa ggctgtttct
     4021 atattgagag tccctccatg cgcggactgc tgcagaaact gaagtgcagt gattatctga
     4081 cacttgtagc agcaagctct gtcatcaggc ccggtgtggc gcgttcaggg atgatgcggc
     4141 agttcatcta tcgcttccat caccctgaca acttcgagta tatccacccg gtgatgaagg
     4201 atcttctcag cgagacattc ggggtgatgg tctaccagga ggatgtgctg aaggtgtgtc
     4261 atcacttcgc cggcctcgat ctctctgatg ccgacacgct caggcgtgcc atgagcggca
     4321 agtaccggtc gcgcagggag atgcagaaga tcactgaacg ctttttcagc aactgccgtg
     4381 acaagggcta tcccggggag atcacaaagg aggtgtggcg ccagattgag tcgtttgcag
     4441 gatactcttt ctccaaggcc cactctgcct catacgctgt ggagagcttc cagagcctct
     4501 ggctgaaggc acactacccc ctcgaattca tggtggccgt aatcaacaac ttcggagggt
     4561 tctaccgtac ctgggtttat gttcaggagg cccgtcgcga gggagccgtc attgaagccc
     4621 cctgtatcaa ccg
//
LOCUS       DAOPBK010000180         4618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15497_length_4618_cov_5.280298, whole genome shotgun sequence.
ACCESSION   DAOPBK010000180 DAOPBK010000000
VERSION     DAOPBK010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15497_length_4618_cov_5.280298"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLA17_09535"
     CDS             complement(<1..444)
                     /locus_tag="PLA17_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPK85693.1"
                     /translation="MAISPVDGRYGERVAELVPFFSEFGLIRYRVLVEIEYFIALAHA
                     GLPQFEGFPAEKEKELLAVWNSFSPADADFIKMKEAVTNHDVKAVEYFVKEQITAIGL
                     EKWSEFVHFGLTSQDINNTAMPMAIRDSVREVMTPMLVSLRESLIR"
     gene            569..1336
                     /locus_tag="PLA17_09540"
     CDS             569..1336
                     /locus_tag="PLA17_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPK85694.1"
                     /translation="MEKIRVSAVKYINSYPFMIGLRDSEVAAMIDLGVCHPSECANRL
                     IEGKADIGLIPVAMVAGMKEYHLVSDYCLSTNGEVRTVLLVSNTPFDEINTIYLDYRS
                     RSSVTLCRILAAKAWKREYRWFPAPEEFDITAMKHGEAAVMIGDRCFEYARHFRYRLD
                     LGLEWKKFTGLPFVFACWVSTRQLPEQFTALFNKALSEGLERRNESVTLLQEDSSATP
                     EEVISYLNHNIDFSLDERKREALAKFIEYIKELNITP"
     gene            1519..3000
                     /locus_tag="PLA17_09545"
     CDS             1519..3000
                     /locus_tag="PLA17_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPK85695.1"
                     /translation="MKKILFIFILIPCTIAAIAGSATLSVTPGNDTVPDSSVIARAGQ
                     VSHNSNTLIYKCEMKKTIAAPLWRSVKKSFAEADSLNADFMLIHMNTYGGQVDIADSM
                     RTLILNYDIPVLVFIDNQAISAGALIAVAADSIYMRSGAGIGAATVVDQSGQKMPDKY
                     QSFMRGMMRATAEAHGKKPVIDGNDTTWVWHRDPEIAQAMVDPSIVIRGIVDTGQVLT
                     LTTLEAIKYGFCEGQAETVKEVIEKAGIKEYTIKELKLTLVDKIIMFLVNPVVSGLLI
                     MLIIGGIYFELQSPGVGFPLAAALFGALLYFAPLYLEGIAANWHIVIFVIGVILLLVE
                     IFAFPGFGVTGVLGIIGIVTGLALVMVDKIVFRFGPSIDGVKEITGAFAIVILSAVLS
                     FLFSLWLSRKLFAPNRLFGSLALETTVDKADGFESFDTGKLQSLVGSHGVAHTVLRPS
                     GKVMVGNEIYPAVAETGFISRGTDVIVRREEQGQLYVVAADKA"
     assembly_gap    3076..3175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3319..3564
                     /locus_tag="PLA17_09550"
     CDS             3319..3564
                     /locus_tag="PLA17_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPK85696.1"
                     /translation="MARTLTFSQLRKIKDQLPDGSIRKIADKLDLEEETVRNYFGGWN
                     FDRGQSAGIHIEKGPEGGIVTITDTTILDLAESMIAR"
     gene            3628..4263
                     /locus_tag="PLA17_09555"
     CDS             3628..4263
                     /locus_tag="PLA17_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK85697.1"
                     /translation="MEHPFIITAEEKYGKLLREKCTQIFSGTDMPSHDHLHHDRVWKN
                     AASLISRLADAGLITDENLAVKAIIASFFHDTGLTVNRGPDHGIESRHICSAFLETTG
                     LTEEERHEILDAIEKHDNKEYTGNSDPCSLAAIISVADDMDAFGEAGIGRYEEIYSMR
                     GIPATEMPGLIVANVLSRLRHLESTYKIFPDLIAEMRAEAETVINHFTDKT"
     gene            4358..>4618
                     /locus_tag="PLA17_09560"
     CDS             4358..>4618
                     /locus_tag="PLA17_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85698.1"
                     /translation="MKRLITAILSIIVLISGVSAQQSNDTPVLRYDMNKSLSWEEAIG
                     FYSDLDKKYSEATLMEMGMTDVGRPLHLFIISSDGETDPAEIR"
BASE COUNT         1247 a         1062 c         1127 g         1082 t
ORIGIN      
        1 cctgatcagg gactcacgca gcgagacaag catgggcgtc atgacctccc taacggagtc
       61 acggatggcc atgggcatag ccgtgttgtt tatatcctgc gatgtaagcc cgaaatgaac
      121 gaactcagac catttctcca gcccaatggc tgtgatctgc tccttgacga agtattcaac
      181 agccttgaca tcatgattgg taaccgcctc cttcatcttg ataaagtcag cgtcagccgg
      241 agagaatgag ttccatacag ccagcagctc cttttccttt tctgcaggga agccttcgaa
      301 ctgcggcaat cctgcatgtg ccagcgctat aaaatattca atctcaacca gtacgcggta
      361 gcgtatcagg ccaaactccg aaaagaatgg caccagctct gccacccgct caccgtagcg
      421 gccatctacc ggtgaaattg ccattaaacg atccatatct gtctctcctc aacccggttt
      481 tacctctgtt ttaaaaacca aaaatagtat tttgttctgg tctgaaaaaa ttgaaccttt
      541 gcatttacct gcgggatgaa tataagagat ggaaaagata agggtttcag ctgtaaagta
      601 cataaacagc tatccgttca tgataggcct gcgtgacagt gaggtcgcag ccatgattga
      661 cctcggtgtg tgtcatccat cggagtgtgc caacaggctc attgagggaa aggcagacat
      721 tggactgata ccagtggcga tggttgcagg tatgaaagag tatcatcttg taagtgatta
      781 ctgcctgagc accaacggag aggtgcgtac ggtccttctc gtaagcaaca ccccgtttga
      841 tgagatcaat accatatatc ttgattaccg ctcacgtagt tccgtgaccc tttgccgcat
      901 cctcgcggcg aaagcctgga agcgtgagta caggtggttc ccggcaccgg aggagtttga
      961 catcaccgcc atgaaacacg gtgaggctgc cgtgatgata ggcgaccgct gtttcgaata
     1021 tgccagacat ttcaggtaca gacttgacct tggcctggag tggaaaaaat ttaccgggct
     1081 gccgtttgta tttgcctgct gggtatcaac gcgccaactg ccggaacaat tcaccgctct
     1141 tttcaacaag gcgctgagcg aaggactcga aagaaggaat gaatcagtca cactcctgca
     1201 ggaggactcc tcagccacac ctgaagaggt tatctcctat ctcaaccata acattgactt
     1261 cagccttgat gagaggaaga gggaggctct tgcaaaattc attgaataca ttaaggaact
     1321 aaatataacc ccatgaatcc cacatgcatc ggatatacaa acataaactc agatattgtt
     1381 caggcatgtg ggttccaaca aacctttaaa gtcagaatta tattatatta aaacccacat
     1441 gtatgagata tacaaccatg aattcagata attttcaatc atgtgtggtt ccagccgacc
     1501 tttaaaaact atatgcggat gaaaaagata ctattcattt ttattcttat tccgtgcacc
     1561 atagcagcaa ttgcaggtag tgccaccctg tcggtgacac cggggaatga tacggttccc
     1621 gattcctcag tcattgcccg ggccgggcag gtatcacaca acagcaatac actgatttac
     1681 aagtgtgaaa tgaagaagac tatagctgcc ccgctgtggc gcagcgtaaa gaagagcttt
     1741 gccgaggctg actctctgaa cgctgatttc atgctgattc acatgaatac ctatggtggt
     1801 caggttgata ttgccgattc aatgcgaaca ctcatcctca actatgatat cccggtgttg
     1861 gttttcatcg acaaccaggc tatttccgca ggggcgctga tagccgtggc tgctgacagt
     1921 atctatatgc gttcgggggc cggtatcggg gcagccacag tggttgacca gagcgggcag
     1981 aagatgcctg acaaatacca gtcgttcatg agggggatga tgagagccac tgctgaagcc
     2041 catggcaaga agccggttat tgacggcaat gacaccacct gggtatggca ccgtgatccg
     2101 gagattgcgc aggctatggt agacccctcg attgtcatca gggggattgt tgacacaggg
     2161 caggtactga cactgaccac actggaggca atcaaatacg gcttctgcga aggccaggcc
     2221 gagacagtga aggaagtaat agaaaaggcc ggaataaagg agtataccat aaaggagctg
     2281 aagctgacac tggttgacaa gataatcatg tttctggtta atcccgtagt ctcagggctg
     2341 ctgataatgc tgatcatcgg aggcatctac tttgagctcc agtcgcccgg tgtgggattc
     2401 cccctggcag ccgccctctt cggggcactg ctctacttcg caccactcta cctcgaaggt
     2461 atagctgcca actggcatat tgtgatattc gtaataggag taatacttct gctggtggag
     2521 atatttgcct ttccgggttt cggggttacc ggggtgctcg gaatcatagg cattgtgaca
     2581 ggacttgccc ttgtaatggt tgacaagata gtcttcaggt tcggaccttc gattgacggt
     2641 gtaaaagaga taacaggagc tttcgcaata gttatcctgt cagccgtgct ttcattcctt
     2701 ttttcattat ggctttcgag aaagctcttt gccccgaaca ggctcttcgg cagcctggct
     2761 ctggagacaa cggtggacaa ggctgatgga tttgaaagct ttgacaccgg caagcttcaa
     2821 tcactcgttg gaagccatgg agtggcacat accgtgcttc gcccgtcagg gaaggtgatg
     2881 gtagggaatg agatttaccc ggcagtggca gagacaggct tcatctccag gggaacagac
     2941 gtaatagtgc gcagggaaga acagggtcag ctttatgtgg tagctgcaga taaagcctga
     3001 gaccgacaat gagcgatgag acctgtaatg agcatggagc ctggcaatga gcaatgagcc
     3061 ccgcattgag cggggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagcc
     3181 ccgcattgag cggggagcca ggcattgagc tggaagcccg gcaatgagca atgagaccgg
     3241 tcggccggaa tgagtctctt atatttcaac tttcaatgaa tacttactat atttgaagaa
     3301 atctatccac tttatatcat ggcacgaaca ctaacatttt cacaattgag gaagatcaag
     3361 gaccagcttc ctgacgggag cataagaaag atagctgaca agcttgattt ggaagaggag
     3421 accgtcagga attatttcgg tggctggaac tttgaccgcg ggcagagtgc cggcatccat
     3481 atagaaaaag gacccgaagg gggcatagtc acaataacag acactaccat actcgacctt
     3541 gccgaaagta tgattgcccg ctaattctgc tatttacagg gaaccaaaaa ggcaaaagtt
     3601 cctacttttg cctttttggt tttaatcatg gagcatccgt tcatcataac tgcggaggag
     3661 aagtacggaa aacttctccg ggagaagtgc actcagattt tctccggcac tgacatgcct
     3721 tcgcatgatc acctgcatca tgaccgggtg tggaaaaatg cagcctctct tatcagtcgt
     3781 ctcgcagatg ccggattgat cactgatgag aatctggcag tcaaagctat aatagcctca
     3841 ttctttcatg ataccggact gacggtcaac cgtggccctg accatgggat tgagagcagg
     3901 catatatgca gcgcttttct tgaaacaact ggcctgacag aagaagaacg gcatgaaata
     3961 cttgacgcaa ttgagaagca tgacaacaag gaatacaccg gcaactcaga tccctgctca
     4021 ctggcagcaa taatttccgt ggcagatgac atggatgcct tcggagaggc aggcataggc
     4081 cggtatgagg agatttattc catgaggggt attccagcca cggagatgcc cggcctgata
     4141 gttgcaaacg tgctttcacg cctgaggcac cttgaatcaa catacaaaat attccctgac
     4201 ctcattgcag aaatgagggc agaggccgaa acagtaatca accattttac agataaaacc
     4261 tgatgacacc cacgtgtatc aaacagacta acacctcttt atataatttt cagtcatatg
     4321 ggttacatca gacctttaaa atcaaaatta tatactgatg aaaagattaa ttacagcaat
     4381 cctgtcaata atagtgctca tatcaggcgt atcggcacag caaagcaatg acacacctgt
     4441 cctgagatat gacatgaaca agagcctttc atgggaagag gcaataggct tttacagtga
     4501 ccttgataaa aagtacagtg aggccaccct catggagatg gggatgacag atgtgggccg
     4561 cccgcttcat ctgttcataa tctcctccga cggagagacc gatcctgccg agatacgc
//
LOCUS       DAOPBK010000181         4614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15508_length_4614_cov_6.540031, whole genome shotgun sequence.
ACCESSION   DAOPBK010000181 DAOPBK010000000
VERSION     DAOPBK010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15508_length_4614_cov_6.540031"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLA17_09565"
     CDS             <1..275
                     /locus_tag="PLA17_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK85699.1"
                     /translation="DLIAQKALDQTARMIALGIANSVIFSSPEVIYLFGGLANAGEAL
                     FGPVRKYADELVQPVFRGTFRVEPSGLPEVQAAVLGASALIWKEMK"
     gene            325..978
                     /locus_tag="PLA17_09570"
     CDS             325..978
                     /locus_tag="PLA17_09570"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPK85700.1"
                     /translation="MHIIEVSRLKTEKEIPSMAEVSVRLDELGKGHGIETLNWQSHTY
                     KPEVRFNIAWGEKEIYIKYYVREAHVKAEKGNTNEMVCEDSCVEFFVSPEDDGIYYNF
                     EFNPIGTALMGSGHSRHDSARTDPAIVGNIRRLTTMGSEPFSEISGDISWSITVAIPL
                     ETFFRHNVGSLAGKSFRANFYKCGDRLTTPHFVTWNPVGTEKPDYHRPEFFGILKFV"
     gene            1053..2078
                     /locus_tag="PLA17_09575"
     CDS             1053..2078
                     /locus_tag="PLA17_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPK85701.1"
                     /translation="MNLTDKKYSKYALVREMMETPGIVRSFRPELSIPFIPAFRNKKG
                     LFLTGEGSSRLFPAKRAIFANLRKEFLLPVLTEGCTQALEYNLKDFVVFAASNSGQTK
                     EVIRLVDHLKKKKHDTVFGLTASHNTKLEEFAHATHVLKCGKENAVAATKSVVEQALF
                     YDSLLRNLRGEKMAGLRKTAALMEEVLTAKIDRKIITMMHDADMIWFAGRDNGVAAEL
                     ALKTNEITRKKSGFLEGTFALHGIEEVMNRGDVLIWVAPFEHDRDKFMECLVKGVGVN
                     VIAISGEKTEFPTIVIPDGGDYAEYLQLTAGWNLLVETGISLGIDPDHPVRARKVGNE
                     YVPEAKK"
     gene            2156..3130
                     /locus_tag="PLA17_09580"
     CDS             2156..3130
                     /locus_tag="PLA17_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK85702.1"
                     /translation="MEKVKAVAGIDIGGTSTTFGFVDRSGEILAGGQIITSQYDKPES
                     FVAALYEKMMITAQNPDYDIEILGFGIGAPMGNINKGTIEYAANLPWKGIVPLADLFA
                     RHTPLPVIVTNDANAAAVGEMVFGGARGMKNFVVITLGTGLGSGFVVDGKLVYGHDGF
                     AGELGHTTIRPGRSDRDCGCGRKGCLETYVSATGLKRTVLQLMAERLDDSELRKLSFE
                     ELDSKLICEAARRGDPIALEAFEQTGAMLAFSLANVTVHTNPEAIFLFGGLALAGELI
                     FEPTRRHLEEDILYIYRGRVKLLPSELSTQNAAVLGASSLVWFELETA"
     gene            3175..3543
                     /locus_tag="PLA17_09585"
     CDS             3175..3543
                     /locus_tag="PLA17_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85703.1"
                     /translation="MMNYIIGGLLIVILFSIAYAYLKPHKLHHARPLSTLALKGTYLI
                     YLLVMLVVICFASLRDGGVSQVFDGAEFFVFLVVLFVPTVGIFSRKITRFSGNRVRYN
                     IIFSVINLIMTLTLIVLYRF"
     gene            3602..>4614
                     /locus_tag="PLA17_09590"
     CDS             3602..>4614
                     /locus_tag="PLA17_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPK85704.1"
                     /translation="MIKERFISYVEDSIRKNWEIEALSNYREKGFTYREIAEIMVKQH
                     IAFKEYGIREGDKIALLGRNSANWCAIYLAVVTYGAVVVPILADFKPDDLHNILTHSD
                     STILFADDKLWESLDPEKLKDIQCVKSLDTFEMLMSRSEKITDIYKSLDEKFSQAYPA
                     FSPDDIKFSGIDNEEMAVLSYTSGTTGFSKGVMIPHRSLAANIRYAQANMPLDPGDRV
                     VSFLPLAHTYGCAFEFLFPFTYGCHITILTRIPSPHILIQAFKEIRPRLILSVPLVIE
                     KIYKYQLLPVISKPVMKVLLKIPGIKNILFKKVREKLETSFGGNFKELVIGGAAFNAD
                     AEK"
BASE COUNT         1176 a         1137 c         1268 g         1033 t
ORIGIN      
        1 gcgatctcat cgctcagaaa gctctcgacc agaccgcccg gatgatcgcg ctcgggatag
       61 ccaactcggt aatattctca agcccggagg ttatatacct cttcggcgga ctggcaaatg
      121 caggcgaagc tctcttcggg ccggtacgga agtatgccga cgagctggtg caaccggttt
      181 tccggggcac cttcagggtg gaaccttccg ggctgccgga ggtgcaggca gcagtactgg
      241 gcgcatcagc gctcatatgg aaggagatga aatagagatg gcaggagaga gaggagcgga
      301 caatagataa aacatcaaac caaaatgcat atcatagagg tcagcaggct gaagacagag
      361 aaggagatac cgtcaatggc agaggtctcc gtacgcctcg acgaactggg caaaggccac
      421 gggatagaga cattgaactg gcagagccac acatacaaac ctgaggtaag attcaacatc
      481 gcatggggcg agaaggagat atacatcaag tactatgtcc gcgaggcaca tgtcaaggct
      541 gaaaaaggca acaccaacga gatggtatgc gaggactcat gtgttgaatt cttcgtctcg
      601 ccggaagatg acggaatcta ttacaacttc gagttcaatc ccatcggcac agcgctgatg
      661 ggctcaggtc acagccgtca tgacagcgca cggacagatc cggccattgt cggcaacata
      721 cgccgcctca caacaatggg cagcgaaccc ttttcagaga tcagcggtga catcagctgg
      781 tcaatcaccg tggccatccc gcttgaaact ttcttccggc ataatgtcgg cagcctggcg
      841 ggcaaaagct tcagggccaa cttctacaag tgcggtgaca ggctgaccac ccctcacttc
      901 gtcacatgga acccggttgg cacggaaaaa cctgattatc acaggccgga gttcttcgga
      961 atcctgaaat ttgtctgacc atataggagc cttcatccgc aggagcagca agaccccgca
     1021 taattaattg caaaactgta aaaaacaata tcatgaacct gaccgataag aaatacagca
     1081 aatacgcact ggtgcgtgaa atgatggaaa caccaggcat cgtcagatca ttccgtcccg
     1141 aattgtccat cccgtttatt cctgccttca gaaataagaa aggtcttttc ctgacgggcg
     1201 aagggagcag cagactcttc cccgcgaagc gggctatttt cgccaacctg aggaaggaat
     1261 ttctgctgcc ggtgctaacc gagggatgca cccaggcgct ggagtacaac ctgaaggatt
     1321 tcgtcgtttt tgcggcatcc aactccggcc agacaaagga ggtgatccgc cttgttgacc
     1381 atcttaagaa gaagaagcat gacaccgttt tcggcctgac ggccagccac aatacgaagc
     1441 tggaggagtt tgcacatgcc acccatgtcc tgaaatgcgg caaggagaat gccgttgccg
     1501 ccacaaaatc agttgtggag caggctctct tctatgactc cctgctgcgc aacctgaggg
     1561 gagagaagat ggcgggcctg cgcaagactg ctgctctgat ggaggaggtg ctcaccgcga
     1621 agattgacag gaagattatc acgatgatgc atgatgctga tatgatctgg tttgccggac
     1681 gtgataacgg ggtggctgca gagctggcgc tgaagaccaa cgagattacc cgcaagaaat
     1741 ccgggtttct tgaggggacc ttcgcgctgc atggcattga ggaggtgatg aacaggggtg
     1801 atgtgctcat ctgggtcgct ccgtttgagc atgaccgtga caagttcatg gagtgcctgg
     1861 tgaaaggcgt gggggtgaat gtcatcgcca tttccggtga aaagaccgaa ttcccgacaa
     1921 tcgtcattcc tgacggagga gattatgccg agtacctgca gctgactgcc ggttggaacc
     1981 tcctggttga gacaggcatc tccctcggca ttgaccctga ccaccccgtg cgcgcacgga
     2041 aggttggcaa tgagtacgtt ccggaagcca aaaagtagca tcctgctgcc gggtcacatt
     2101 ttttttcgct tctattaaag cgtatattta cgttgtgtaa actctctttc tgaatatgga
     2161 aaaggtaaaa gcagttgcag ggattgacat cggaggcacc agcaccactt tcgggtttgt
     2221 tgaccggtcg ggagagatcc tggccggggg ccagatcata acgagtcaat atgataagcc
     2281 tgagagtttt gttgctgcgc tctatgagaa gatgatgatc actgcacaga acccggatta
     2341 tgatattgag attctcggtt ttggcatcgg ggctcccatg ggcaatatca acaagggaac
     2401 catcgaatat gcggctaacc tgccctggaa aggtattgtc ccgctggctg atctttttgc
     2461 ccgtcacacc ccgctgccgg tcatcgtcac caatgatgcc aacgctgcag ccgtgggcga
     2521 gatggtcttc ggcggcgcca gggggatgaa gaactttgtg gtgataaccc tcggcaccgg
     2581 cctcggcagc ggattcgtcg ttgacggaaa gctggtgtac gggcatgacg gcttcgcggg
     2641 tgagttggga cataccacca taaggccggg gaggtcagac cgcgactgcg gatgcggacg
     2701 gaaggggtgt cttgagacat atgtctcggc aacaggcctg aaacggacgg tgctgcaact
     2761 gatggccgaa agacttgacg acagcgaact acgaaagctc agcttcgagg agcttgactc
     2821 aaagttgatc tgtgaagctg cgcgcagggg tgaccccata gccctcgagg cgtttgagca
     2881 gaccggtgcc atgcttgcat tcagcctggc caacgtgaca gttcacacca atcccgaggc
     2941 tattttcctc ttcgggggcc tcgccctcgc cggggagctg atatttgaac ccaccagaag
     3001 gcatctggag gaagatatcc tctatatcta ccgtggcagg gtgaagttgc tgccctcgga
     3061 gctgagcacg cagaatgcgg cggttcttgg cgcaagctca ctggtatggt ttgagctgga
     3121 gacagcataa accctgattt tgggaacaaa atgaacaagt ataatctgcc ggagatgatg
     3181 aattatatca taggggggct cttgatagtg atactcttct ccattgccta tgcctatctc
     3241 aagccgcaca agctgcatca tgccaggcct ctctcaacac tggccctgaa aggcacatac
     3301 ctgatatacc tgctggtgat gctggtggtg atctgttttg ccagcctcag ggacggcggg
     3361 gtgagccagg tttttgacgg ggcggagttc ttcgtgtttc tggtagtgct cttcgtgcct
     3421 acggtcggca tcttttcacg aaaaataacc cgcttcagcg ggaacagagt aaggtacaac
     3481 ataatcttca gtgtgattaa cctgataatg accctgacac tgattgtcct gtacaggttt
     3541 tgacaccaga taacggcgaa aagcttacat ttgccgaatg aaataaaaaa actccaaaat
     3601 tatgatcaag gaacgattca tctcctatgt ggaggatagt atcagaaaga actgggaaat
     3661 agaggcgctt tcgaattacc gggagaaagg tttcacgtac agggagatcg ccgagataat
     3721 ggtcaaacag cacatagctt ttaaagagta cggcatccgt gaaggtgaca aaatcgccct
     3781 gctcgggcgg aactctgcca actggtgcgc catatacctg gcagtggtta cctatggcgc
     3841 tgtggtagtg ccgattctgg ctgatttcaa acccgatgat cttcacaata tcctaaccca
     3901 ctccgactca acgattcttt ttgctgatga caagctgtgg gaatcacttg atcccgaaaa
     3961 gctgaaggat attcagtgcg taaaaagcct tgacacgttt gagatgctca tgagccgcag
     4021 tgagaagatc acggatatct acaaatctct tgatgagaag ttttcacagg catacccggc
     4081 attcagtccg gatgatatta aattttccgg tatagacaat gaggaaatgg cagttctgag
     4141 ctatacctcg ggtacaacgg gcttctccaa gggtgtgatg atacctcaca ggagcctcgc
     4201 tgcaaacata cgctacgcac aggcgaacat gcctctggat ccgggtgacc gggttgtttc
     4261 cttcctgcca ctggcacaca cttatggctg cgctttcgag tttctcttcc cctttaccta
     4321 cggatgccat atcaccatcc tgaccaggat accgtcgccg catatactca tccaggcctt
     4381 caaggagatc agacccagat tgatactctc ggtaccgctg gtcatcgaga agatttacaa
     4441 gtatcagttg cttccggtta taagcaagcc tgtcatgaag gtgttgctga agataccggg
     4501 cattaaaaat attcttttca agaaggtgcg tgagaagctt gagacaagct ttggcggcaa
     4561 tttcaaggag ctggttatcg ggggtgctgc attcaacgct gacgccgaaa agtt
//
LOCUS       DAOPBK010000182         4577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15653_length_4577_cov_4.448253, whole genome shotgun sequence.
ACCESSION   DAOPBK010000182 DAOPBK010000000
VERSION     DAOPBK010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15653_length_4577_cov_4.448253"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PLA17_09595"
     CDS             <1..1286
                     /locus_tag="PLA17_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK85705.1"
                     /translation="IYSPALSPRLEYVAGVIFSTVLGIKYEITTDRRKIGSKPAIIYS
                     NEKVKNQFVIRPAGLLGETGVGNVSPEVSFIGEMPLFFETEGGSIPFDLFSAVFYMLS
                     RYEEYLPFSPDAHGRFPGAASLAARGGFLRIPVVEIWSKYLAIELIRRYPVLTIRSNE
                     YSALVTVDVDQPFAYRSRGFLRSMGGLVKGLAGVGARPADRIRTMAGEVPDPYDSFGY
                     IEEKLKESQSEALFFFPTGDRGEHDHNPSFRDRDYGEIIRRYDRIYGSGLHSSYRSAG
                     RIRTLKTEAERYSSITGHLPVKVRQHWLLLKMPDTYRDFSEAGMTHDYTMGYHDEPGF
                     RAGIARPFPFYDLATERATGLTVVPFQVMDVTLRKYMHLQPEAALEVIRTLIAATRDA
                     GGLFVSIWHNTSLNECNGWEGWRALFEEMLLMQKA"
     gene            1283..2188
                     /locus_tag="PLA17_09600"
     CDS             1283..2188
                     /locus_tag="PLA17_09600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK85706.1"
                     /translation="MIRHLKHNEIDRGRWDACLNEIPAVKPYAMSWYLDRISPGWEAL
                     VEDDYHALFPLPCHRKLGISYLTTPLFLQQLGLFTAEGDRKRMAEEFISFMQGFYMLV
                     DLCIGQEAAVPGYTLTPRYNYELKLDDPYEKLWFNYMTDCRRNINIARRYPQEIVENP
                     TPEELIALFRANTARRAGVTRNSPYLRLQALMKHCTSSGLGRILGVRSPRGKLLWGMF
                     VIETPGRTTMLFTAGSRKSRELRTSYLVIDHLIRSYAGTGQIIDFAGSSIPSIAIFMR
                     SFGGDKTTYWRLYRNNLPWPVKLLK"
     gene            complement(2189..2905)
                     /locus_tag="PLA17_09605"
     CDS             complement(2189..2905)
                     /locus_tag="PLA17_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK85707.1"
                     /translation="MEEITPLDTIIELKSCSIMQQDHLVLSDVNLKVEKGEFVYLVGR
                     VGSGKTSLIRTLNAQLPLAEGEGWVAGFSLHTIKKREIPMLRRKLGIVFQDFQLLTDR
                     SVHDNLEFALRATGWNNKIAVEERIKEVLEKVGLQNKGFKMPHQLSGGEQQRVVIARA
                     LLNDPEIILADEPTGNLDPETSEEIHVLLKNISNTGRAVIMSTHKHSLVRKFPGRTFR
                     CENGKLSETDQDDQVIELLF"
     gene            3130..>4577
                     /locus_tag="PLA17_09610"
     CDS             3130..>4577
                     /locus_tag="PLA17_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK85708.1"
                     /translation="MNRKILTVVMVLLIAAGSAVKGQVSFSGDEMTSLLNNGYEMFSK
                     AKYATAIGLFDKWLENGQNGTKLQRADAEYHAALASMRLMAPDAEFRMQRFINANQES
                     PMLNQARFESGRYCYQQRNYSCAIEWFEQTDRIQLNEKEYAEYLFKLGYSQYRRGDTR
                     KAQMLFAELMESETDYTAPATYYHAAIAYENGFFGTALEGFEKLQGDETFGSIVPFYI
                     TQIYYLDKNYDGILEIAPALIDQAGKKRETELYRFIGDAYFQKGNYSEAIPYLEKFIR
                     ETKLSDRNDKYELAYCYYQTGAIGQATKLFNEVVGRSDILTQNAYYMLGSCYLQAGDK
                     KKAQMAFSAASKMNFDADVQEEALFNFAKLAYENSYAPFGEGIDALHDYIRTYPNSEH
                     VSEAYDYLISAYMRLKNYQAALNSLEKISSKDDRLKEAYQKVAYFRGIEQFRNRQYAE
                     AVSMFDRSLTYREKNPDLYARALYWRGESNYRL"
BASE COUNT         1130 a         1109 c         1235 g         1103 t
ORIGIN      
        1 ggatttactc tccggctctc tctccccggc ttgagtatgt ggcaggtgtg atcttcagca
       61 ccgttctggg tataaaatat gagataacaa ctgacaggag gaagataggc agcaaacctg
      121 ccataatcta ctccaatgaa aaggtaaaga atcagtttgt tatccgtccg gcggggctcc
      181 ttggtgagac aggtgtcggc aatgtcagtc cggaggtgtc atttatcgga gagatgccgc
      241 tcttttttga aactgaaggg ggaagcatcc cgtttgatct tttttctgct gtattctaca
      301 tgctgtcgag gtatgaggaa tacctccctt tttcgcctga tgcccacggg aggtttccgg
      361 gtgccgcatc gctggccgcc aggggaggat tcctgaggat tcctgtcgtt gagatatggt
      421 cgaaatacct ggcgattgaa ctgataagac ggtatccggt ccttacaata aggtccaatg
      481 agtacagtgc cctggttact gtggatgtag accagccttt tgcataccgc agcaggggat
      541 ttctcaggag catgggtggt ctggtgaaag ggcttgccgg cgttggagcc aggcctgctg
      601 accggatcag aaccatggcc ggagaggtgc cggatccgta tgacagcttc ggatacattg
      661 aagagaagct gaaggagagc caatccgaag ccctcttttt ctttcccacg ggtgaccggg
      721 gtgagcatga ccataatcct tccttccgtg atcgcgatta cggcgaaatt atacgcaggt
      781 atgaccggat atatggcagc ggccttcatt catcgtacag gtctgctggc aggatcagga
      841 cactgaagac ggaagcggaa cgctacagct ccattaccgg gcatctgccc gtgaaggtac
      901 gccagcactg gctgttgctg aagatgccgg acacataccg tgacttcagt gaggcaggca
      961 tgacgcatga ctatacaatg ggttatcatg atgagccggg tttcagggca ggcatcgccc
     1021 ggcccttccc cttctatgat cttgccacgg agagggcaac cggccttact gtggttccat
     1081 ttcaggtgat ggatgtgacc ctcaggaaat atatgcatct tcagcctgaa gctgccctgg
     1141 aggttatcag gactctcata gccgcaacaa gggatgcagg cggcctgttt gtttcaatat
     1201 ggcacaacac ctccctgaat gaatgcaatg gctgggaggg ctggcgagct ctgtttgagg
     1261 agatgctgtt aatgcagaag gcatgatcag acatctgaaa cacaatgaga tagaccgtgg
     1321 gcggtgggat gcatgcctga atgagatccc ggcggtcaaa ccttatgcca tgtcatggta
     1381 cctggataga atttcaccgg ggtgggaggc acttgtagag gatgattatc acgcgctttt
     1441 tcctcttccg tgccacagga aactggggat cagctatctc accacaccgt tgttcctaca
     1501 gcaactgggg ctcttcaccg cggagggtga ccggaaacgg atggctgagg agttcatctc
     1561 gttcatgcag ggcttctaca tgctggttga cctctgcata gggcaggagg ctgcagtgcc
     1621 gggctacact cttacaccca ggtacaatta tgagctgaag ctcgatgatc cctatgaaaa
     1681 gctgtggttc aactatatga ccgactgccg gaggaacatc aacattgcac gccgctatcc
     1741 acaggaaata gttgagaatc ccacccccga agagctgatt gcgctctttc gtgccaatac
     1801 tgccagacgg gccggggtga caaggaattc gccatacctg agacttcagg ccctgatgaa
     1861 gcattgcacc tcatccggac tcggaaggat tctgggtgtg agatcgccca ggggaaaact
     1921 cctgtgggga atgttcgtga ttgaaacacc cgggcgcaca acgatgcttt ttacggcggg
     1981 gagcaggaaa agccgcgaac tgcgcacatc ctacctcgtc atagatcacc tgatcaggag
     2041 ttatgcgggc accggacaga tcatcgattt tgcaggctcg tcaattccgt cgatagccat
     2101 cttcatgaga tcattcggag gtgacaaaac aacatactgg aggctgtacc gcaacaatct
     2161 cccatggccc gtgaagcttc tgaaatgatc agaagaggag ttctattacc tgatcatcct
     2221 gatctgtctc tgacagcttg ccgttttcgc accggaaggt ccttccgggg aattttctga
     2281 ccagtgagtg cttgtgggtt gacatgataa cggcccggcc ggtattgctg atattcttca
     2341 gcaggacatg tatctcctct gatgtttccg gatcaaggtt gccggtcggt tcatcggcca
     2401 ggattatctc aggatcattc agcagggcac gtgcaatgac taccctctgc tgttcgccgc
     2461 ctgagagctg gtgaggcatt ttgaatccct tgttctgcag cccgaccttc tcaagcacct
     2521 cttttatccg ttcttcaacc gcaatcttgt tgttccatcc cgttgccctg agggcaaact
     2581 ccaggttgtc atgcactgac ctgtcggtca ggagctggaa atcctggaac actatcccga
     2641 gttttcttct cagcataggt atctccctct tttttatcgt gtggaggctg aaccctgcca
     2701 cccatccttc gccttcggcg agaggaagct gtgcattgag ggtgcggata agcgaagttt
     2761 tgccgcttcc caccctgccc accaggtaga caaattcacc tttttcgacc ttcaggttca
     2821 catcactgag gacgaggtga tcctgctgca ttatcgaaca gcttttcaat tcgatgattg
     2881 tgtcgagagg ggtgatctct tccattcgga acagtttttg tgcaaattaa aaaagaatta
     2941 acaataagca ctctctgtgt tgataaaatg caggttaagg aagattccgc aaggcattat
     3001 aattgtattt ttacgactga taaaactgcg ccgaaaagtc cgcgcaatat attgaatata
     3061 aatcaaataa ccacaggggt aacctgcagg tgattgttat ttgagacagt ttgagaaaag
     3121 gataaagtta tgaacaggaa gatactgacg gtggtcatgg tcttactcat tgccgcaggg
     3181 tcagctgtga aggggcaggt ctccttttca ggggatgaga tgacctcgct gctgaacaac
     3241 gggtatgaga tgttttcaaa ggcaaagtat gctacggcta tcgggttgtt tgacaaatgg
     3301 cttgagaacg ggcagaacgg aaccaaactt cagagggcag atgcggagta tcacgctgcg
     3361 ctggcctcga tgaggctgat ggcacctgat gccgagttca ggatgcagcg gttcatcaat
     3421 gccaatcagg agagcccgat gctcaaccag gcacggtttg aatcgggccg ctattgttac
     3481 cagcagcgta attacagctg tgccattgag tggtttgaac agacagaccg catccagctg
     3541 aatgagaagg agtatgccga gtatcttttc aagctggggt actcgcagta ccggaggggt
     3601 gacaccagga aggcacagat gctctttgcc gaactgatgg aatcagagac cgattatacc
     3661 gcgccggcaa cttattacca cgccgcaatt gcctatgaaa acggattctt tggtacagcc
     3721 ctggaaggct ttgaaaaact gcagggtgat gaaacattcg gcagcattgt cccattctac
     3781 attacccaga tctattacct cgacaagaac tacgatggta tacttgagat agcgcctgca
     3841 cttattgacc aggcagggaa gaaaagggag accgagcttt accggttcat cggtgacgca
     3901 tattttcaga aaggcaatta cagtgaagcc atcccttacc ttgaaaaatt catcagggag
     3961 acaaagctca gtgaccgcaa tgacaaatac gagctggcat actgttacta tcagaccggg
     4021 gccattggtc aggcaacgaa gcttttcaac gaagtggttg gccgcagtga tatcctgacg
     4081 cagaacgcct attacatgct gggctcgtgc tatcttcagg caggcgacaa gaaaaaggcg
     4141 cagatggctt tctcggctgc ctcaaagatg aacttcgatg cggatgtaca ggaggaggcg
     4201 ctgttcaact tcgcaaaact ggcttatgag aattcatacg ctccctttgg cgagggtata
     4261 gatgcactgc atgactacat tcgcacctat cccaactcag aacatgtgtc agaggcctat
     4321 gactacctga tatcggccta tatgaggctg aagaattacc aggctgcact gaactcgctt
     4381 gagaagataa gcagcaagga tgaccgcctg aaggaggcct accagaaggt ggcctatttc
     4441 cgtggcatag agcagttccg caacaggcag tatgccgaag cggtatcaat gttcgacagg
     4501 tcactcacct acagggagaa gaaccctgac ctttatgccc gcgcccttta ctggagaggt
     4561 gagtcgaact accgtct
//
LOCUS       DAOPBK010000183         4512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15914_length_4512_cov_3.960960, whole genome shotgun sequence.
ACCESSION   DAOPBK010000183 DAOPBK010000000
VERSION     DAOPBK010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15914_length_4512_cov_3.960960"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1715
                     /locus_tag="PLA17_09615"
     CDS             84..1715
                     /locus_tag="PLA17_09615"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPK85709.1"
                     /translation="MMKNQTTPVSVRTGLVILLALFPLIATGQLIKDIDPDGPGYAEN
                     CTCIMVGRLASTDGSVMTSHSCDGRYRTWLEMVPAMTFARDTVHAVYRGTMQTEEAWD
                     MTNLTRKGEIPQVKSTYAYLNTAYPCLNEKQLAMGETTIYGKRELINQEGLFVIEELE
                     RIALQRCTTARQAIALIGSLAEQYGYGDLAECLTIADPKEVWQLEIAGSGPGKPSAMW
                     VAQRIPDEHVGIAANIPRISDVNFRDSDNFMTSTDLRKKAKELGYWDGKEPFKFYRVI
                     GTGKPYAIREFFVLSSLAPELNLTMEMEELPFSVKPEKKLSASDVMALFRETYEGTPY
                     DMTRNLMVTVRKKDEAGNEITEMVKSPAISNWMNNDVRTLINELKPGTIDRQRTIAIA
                     GCSYSHVIQCREWLPDEVGAVAWFSFDNPGQSPRIPIFSGIISLPKSFSICGQHRYRE
                     DAALWAFREANRLSMINWERGRKLIEPAVAELENKALTELPGLEERVKIMVKEGRNEE
                     ARKVVTEYSNAFAYGAMNRWQELKRELWQMFGRGF"
     gene            1777..2874
                     /gene="hydE"
                     /locus_tag="PLA17_09620"
     CDS             1777..2874
                     /gene="hydE"
                     /locus_tag="PLA17_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPK85710.1"
                     /translation="MEMGSGIASILDKKALDRDDIIALLQCGEDEAPLLYRRAAAVRD
                     HYVGSHVYLRGLIEYSNICRKNCLYCGVRRDNLRIERYTMTREEVLEAAMTAFNLKLG
                     SIAIQSGENSSGAFIDTIEELISTIRRMTDNKLGITLSLGEQSRETYRRWYDAGAHRY
                     LLRIETSNQSLYRKLHPDDEMHRYQRRLECLKAIQDTGYQTGTGVMIGLPYQTMGDLA
                     DDIIFMRDFDIDMCGMGPYIEHSDTPLGERGTDHRLLKERFNLSLRMIAILRIVMRDI
                     NIVASTAMQTIDPAGREKAVSCGANVVMPNLTPARYREGYRIYEGKPGYNEVNESNVS
                     GLNPDLIPGITIGLGIWGDTPHYGRRLGRKK"
     gene            2952..>4512
                     /locus_tag="PLA17_09625"
     CDS             2952..>4512
                     /locus_tag="PLA17_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPK85711.1"
                     /translation="MMKKTALSSVIILVSLISFGQERLTLEYYMPAGMTFDSSIPVPG
                     SIGHEVGEWHVTHDKLAGYMKLLGDLSGRAVWEDYGKSWEGRPLGQLIITSEKNMARL
                     EEIRLEHLKLSDPSVSKSVTTADMPLIIKLGYGVHGNESSSSNASILTAYYLVAGRGP
                     QIDEILDKCVILIDPSLNPDGLQRHSAWVNMNRGMTLATDPSGREFNEPWPGGRTNHY
                     WFDLNRDYLMLQHPESVGRVEAFYRWMPNINTDHHEMGASATFFFQPGVQSRNNPVVP
                     PDNQALTTEIGSYHAKYLDSIGSLYYTGESFDDYYLGKGSSYPDAHGSIGILFEQAGT
                     KGHLREVPGGVLSFPFAIRNQFVVSLSTIEAGMNMRVKLLDMQREFYLSALREADASP
                     VKGYLFSAGADPGGCARFIENLLRHRIEVYRCGRQITKNGITYSPENSYFVPARQKEY
                     RFVRALFETVKTFSDTVFYDISTWVMPMAFNLSYTPLGSAETTGIAGGRVTSPPFPDG
                     AVKGTEKDYAWL"
BASE COUNT         1186 a         1187 c         1193 g          946 t
ORIGIN      
        1 gaagccgcgg cagtatgttg cagaatatta tctttggctg cattatgttg aataagccga
       61 ctatttttgt cagcaaacaa ccaatgatga aaaaccagac cacacctgtc tctgtcagaa
      121 caggattagt aatccttctg gcactattcc cgcttattgc taccggacaa ttaataaagg
      181 atattgaccc tgacggtccc ggctacgccg aaaactgcac ttgcataatg gttggccggc
      241 tggcctcaac cgacggctcc gtcatgacct cgcactcctg cgacggcagg taccgcacat
      301 ggctcgagat ggtgccggca atgaccttcg caagagatac cgttcatgct gtctaccggg
      361 gaacaatgca gaccgaggag gcatgggaca tgaccaacct gacacgcaag ggcgaaatcc
      421 cacaggtgaa atccacatac gcatatctca acacggcata tccatgcctc aacgagaagc
      481 agctcgccat gggcgaaaca accatttacg gaaagaggga actgataaac caggagggcc
      541 tcttcgtgat agaggagctt gagaggatcg cgcttcagcg ctgtacaacg gcacgccagg
      601 caatagccct cataggttcg cttgccgagc agtatggtta cggtgatctg gccgaatgcc
      661 tcaccatagc tgacccgaaa gaggtgtggc agctcgagat agcaggctcg ggaccgggca
      721 aaccctcggc aatgtgggtg gcgcagagaa ttcctgatga acatgtaggc attgctgcca
      781 acataccaag aatctcagat gtcaacttca gggacagtga taacttcatg acctcaaccg
      841 acctccggaa aaaggcaaaa gagctcggtt actgggacgg aaaggagccg ttcaagttct
      901 acagggtgat cggcacgggc aaaccgtacg ccatccgcga attcttcgta ctgagctcac
      961 ttgcaccaga acttaacctg actatggaga tggaagaact ccccttctcc gtcaagcctg
     1021 agaaaaagct ctccgccagt gacgttatgg cgctcttccg cgaaacctac gaaggtacac
     1081 cctatgacat gaccaggaac ctgatggtta ccgtacggaa aaaagatgaa gccggcaacg
     1141 agataactga aatggtgaag tcgccggcca tcagcaactg gatgaacaat gacgtacgca
     1201 ccctgatcaa tgagctcaag cccggtacca ttgacaggca gcgcaccatt gccatcgcag
     1261 gctgctccta ctcgcacgtg atacagtgcc gtgagtggct gcctgatgaa gttggcgcag
     1321 tggcatggtt ctcatttgac aatcccgggc agagtccacg cataccaatc ttctcaggga
     1381 taatctctct gccgaagagc ttcagcatct gcgggcagca ccgctaccgt gaagatgccg
     1441 ccctctgggc cttccgcgaa gccaacaggc tctcgatgat taactgggag cgtggccgca
     1501 agttaataga gccggcagta gctgaactgg agaataaagc gctgacggaa ctgcccggac
     1561 tggaggagag agtaaagatc atggttaagg agggcaggaa tgaagaagcc aggaaagtgg
     1621 tgacagaata ctcaaatgcc ttcgcttatg gtgccatgaa caggtggcag gaactgaaaa
     1681 gagaactctg gcagatgttc ggcagaggtt tctgatcata ataccccctg ccctgaactt
     1741 atcatattcc gaattgttaa ctttagacaa ttttcaatgg aaatgggttc aggcatagcc
     1801 tcaattcttg acaagaaagc ccttgacagg gatgacatca ttgcacttct tcagtgcggc
     1861 gaggatgagg ctccccttct ataccggcgg gcagcagccg tcagggacca ctacgtgggc
     1921 agtcatgttt acctgcgcgg actcattgaa tattcaaaca tctgcaggaa aaactgcctc
     1981 tattgcggcg tcaggcgtga caatctcagg atagaaaggt acactatgac acgggaagag
     2041 gttcttgagg cagccatgac tgccttcaat ctcaaactcg ggtcaattgc catccagtca
     2101 ggagagaact cctccggagc atttattgac accattgaag agcttatatc cacaatacgc
     2161 aggatgactg acaataagct cggtattacg ctctctctag gcgagcagag cagggagacc
     2221 tacaggagat ggtatgacgc gggcgcacac cgttacctgc tgaggataga gacatcaaac
     2281 cagtccctct accggaagct gcatcctgac gatgagatgc acaggtacca gagaagactt
     2341 gaatgcctga aggcgattca ggataccggg taccagacag gtaccggtgt gatgataggg
     2401 ctgccgtacc agactatggg tgatctggct gatgatatca tcttcatgcg cgattttgac
     2461 attgacatgt gcgggatggg accgtatata gagcattcag acaccccgct gggggaaagg
     2521 ggaaccgacc atcgcctcct gaaggaacgc ttcaacctct ctctcaggat gatagcaata
     2581 ctcaggattg tcatgaggga tatcaatatc gttgcttcaa cggccatgca gaccattgat
     2641 ccggcaggcc gtgaaaaggc tgtttcctgc ggagccaacg tggttatgcc gaacctcacc
     2701 cctgccaggt acagggaggg ataccggatt tatgagggca agccgggcta caacgaggtg
     2761 aatgaatcaa acgtcagcgg cctgaacccc gatctgatac ccggaatcac catcgggctg
     2821 gggatatggg gtgacacacc ccattacggc aggaggctgg gcagaaaaaa atgattatgc
     2881 tgcagaacaa ggttctgcca taacaaccaa tcctcttttt tccgaaattg ctcacactac
     2941 tcaaacctga catgatgaaa aaaacagccc tttcatcagt gatcatcctt gtgtcactga
     3001 tcagtttcgg ccaggagagg ctcacccttg aatactacat gcctgcaggc atgacttttg
     3061 acagcagcat cccggtgccg gggtccatcg gtcatgaggt gggcgaatgg catgtcaccc
     3121 atgacaagct ggccggttac atgaagcttc ttggcgacct ctccggcagg gcagtgtggg
     3181 aagattacgg caaaagctgg gaagggaggc cactcgggca gctgatcatc acatcggaga
     3241 agaacatggc ccgtctcgaa gagatcaggc tcgagcacct gaaactatca gatccttcag
     3301 tctcaaagag tgtcaccact gcagacatgc cgcttatcat caagcttggt tacggcgtgc
     3361 atggcaacga gtcaagctca tccaatgctt caatacttac agcctactac ctcgttgccg
     3421 gcaggggacc gcagatagac gaaatacttg acaaatgcgt catacttatc gatccgtcac
     3481 tcaatcctga cggtttgcag cgtcactctg cctgggtgaa catgaaccgc gggatgacgc
     3541 tcgcaaccga cccttccggg agggagttca atgagccatg gcccggaggg cgaaccaacc
     3601 actactggtt tgacctgaac cgtgactacc tgatgcttca gcaccccgag agtgtggggc
     3661 gcgtggaagc tttctaccgc tggatgccca acataaacac tgaccatcat gagatgggtg
     3721 cctcagccac cttcttcttc cagccgggcg tgcagtcgcg caacaacccg gtggtcccgc
     3781 ctgacaacca ggctctgacg accgagatag gcagttacca tgcaaagtat cttgattcaa
     3841 tcggaagcct ctattatacc ggcgagagct ttgacgacta ttacctgggc aaaggctcat
     3901 cctaccctga tgctcacggg tcaataggta tcctcttcga gcaggccggc acaaagggtc
     3961 acctgcgcga agtaccgggc ggagttctct ccttcccctt tgcaatcagg aaccagtttg
     4021 tagtatcact atccaccata gaggcaggga tgaatatgag ggtaaaactg cttgacatgc
     4081 agcgcgagtt ctatctctcg gctctcaggg aggctgacgc ctcaccggta aaaggttacc
     4141 tgttcagtgc cggtgccgac cccggagggt gtgcacgctt tatagaaaac cttctcaggc
     4201 acaggatcga ggtttacaga tgcggccgcc agataaccaa aaacggcatc acctatagcc
     4261 cggagaacag ttattttgtt ccggccagac agaaggagta ccgttttgtc agggcgctgt
     4321 ttgaaactgt aaagactttt tctgacaccg tgttttatga catttccacc tgggtaatgc
     4381 cgatggcttt caacctgagc tatacccccc ttggcagcgc tgagacaaca ggcatagccg
     4441 gcgggagggt gacctctccg ccattccctg acggggctgt aaaaggtacc gaaaaagatt
     4501 atgcatggct ct
//
LOCUS       DAOPBK010000184         4506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15936_length_4506_cov_4.721186, whole genome shotgun sequence.
ACCESSION   DAOPBK010000184 DAOPBK010000000
VERSION     DAOPBK010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15936_length_4506_cov_4.721186"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1015)
                     /locus_tag="PLA17_09630"
     CDS             complement(248..1015)
                     /locus_tag="PLA17_09630"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK85712.1"
                     /translation="MMNKLLVFTAVTLMMTSCAPKEKENWIPLFNGHNLDGWIIKIKG
                     SPLGENYKNTFRVEDSVMKVSYAEYGTFMNEYGHIYYREPFSSYKLRVEYRFTGEQTP
                     GGPSWAFRNSGIMFHCQDPATIGLDQDWPVSVEVQFLGSTADRESPTMNVCTPHTHIV
                     IGDVLITEHCTSSSSEFYYDDAWVTAELVVYADSIIHHIVNGDTVMTYSKPQVGGDLP
                     EGFTLPRGTPLKEGYIALQSESHPVEFRKVEILKLRQ"
     gene            complement(1200..3098)
                     /gene="dxs"
                     /locus_tag="PLA17_09635"
     CDS             complement(1200..3098)
                     /gene="dxs"
                     /locus_tag="PLA17_09635"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPK85713.1"
                     /translation="MSSSERLLEKINTPDDLRKLKRLDLVRLSNELRQHIIDEVSHNP
                     GHLGASLGVVELTVALHYVYNTPYDKIVWDVGHQAYGHKILTGRKTQFNTNRKYKGIS
                     GFPRMSESEYDAFGVGHSSTSISAALGMAVAAQAKGEKRKVVAVIGDGALQAGLAFEG
                     LNNAGNQKADLLVILNDNNISIDASVGALKEYLLDITTSRTYNRFKDKIWRLLGRYKR
                     VGIRARVLASQLEGGIKGTVLKQSNLFEAFKFRYFGPIDGHDVLHLTRVLEDLKTIPG
                     PKLLHCVTVKGKGFPKAEEQQTIFHAPGIFDKCTGELIRPAEGPKHSLYQEVFGYTIV
                     ELARQNEKIVGITPAMPSGCSLNIMMKEMPDRAFDVGICEQHAVTFSAGLAANGLLPF
                     CNIYSSFMQRAYDQVVHDVALQRLKVIFCMDRGGLVGPDGATHHGFFDIAFMRNIPGM
                     IASAPMDEVELRNMMYTAQLDTVKGPFSIRYPRGAGVIADWHQPFREIPVGRARLIRE
                     GSDIAVLSLGHPGNFVVEAAARLSEKGISILHYDMRFVNPLDEQVLHSVMNQFKKIIT
                     VEDGMIKGGFGSAVLEFANDHGYPVEIIRLGIPDYFVEHGSQQELWHECGYDVAGIEQ
                     AIVQALEG"
     gene            3365..4378
                     /locus_tag="PLA17_09640"
     CDS             3365..4378
                     /locus_tag="PLA17_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK85714.1"
                     /translation="MKAPVIRLSFLLLALIAVSCVTGKRYNTLLERNLMLMSERDSLK
                     AENNWLTMSNRELTAKSMQLAEDTERLTREVEKARADLAQSRDDVSRLSTRYDELQRA
                     QAELVAGKAQETQRLLAELRQAQTELQQREDLMRDLEMNLDTKKATLEELTYELEKRN
                     IRLAELEKMLDAQQKAVKALKDKVSEALFGFENNGLTVSMKNGQVYVSMDDKLLFKTG
                     SYEIDANGRTALRKLGALLEKNTDVTITIEGHTDDVPYRAAPGQQILDNWDLSVKRAT
                     TVVRVLIQDTHINPKRFVASGRSEYIPVDPAKTPDARQKNRRTEIILTPDLTALYRIL
                     ESY"
BASE COUNT         1098 a         1159 c         1130 g         1119 t
ORIGIN      
        1 taagtcagcc ccccgggctg ccttttgacg ctcggtcctg ccatccgggc ataatacagt
       61 cctgcagttc ctgataagct tcactgtcag cggctttata tccgccattc gctgatactc
      121 atttacggac ctgcaggatg cgaaattaga ataaaaattt gaaaacgcca ttactccctt
      181 gcaccttccc tgcaacctgg ttaattcact acaatgaccc gccggtcttc tttatacact
      241 tacccctcta ctgcctgagt ttcagtatct ccacctttct gaattccacc gggtgtgatt
      301 cagactgcag ggctatgtaa ccctccttaa ggggagttcc ccggggcagg gtgaagccct
      361 caggcagatc gccacccacc tgcggctttg aataggtcat aaccgtatca ccgttgacaa
      421 tatgatgaat gatactgtca gcatacacaa ccagctctgc cgtcacccag gcatcatcat
      481 aatagaattc tgaggaagag gaggtgcagt gttccgttat cagcacgtca cctattacaa
      541 tgtgcgtgtg cggtgtgcag acgttcatgg tgggagattc acggtcggca gtgctgccca
      601 ggaactgcac ttcaactgat acgggccagt cctgatccag tcctatagtt gccggatcct
      661 ggcagtggaa catgatgccg ctgttgcgga atgcccacga cgggccacca ggtgtctgtt
      721 caccggtgaa gcggtattcg acacgcagtt tgtatgatga gaacggctcc ctgtagtaga
      781 tgtggccgta ctcattcatg aatgtgccat actctgcgta tgagaccttc atgacactat
      841 cttcaaccct gaatgtgttt ttgtagtttt ctcccagggg tgatcccttg atcttgatga
      901 tccagccgtc aaggttatgg ccattgaaga ggggaatcca gttctccttc tctttcgggg
      961 cacaggaggt catcatcagg gtgactgcgg taaagacaag aagtttattc atcattcgat
     1021 ctgcaatgtt taatggctca gggccatgtt tttcatcact atgttcttct gcaatgctaa
     1081 atagttcagg gccagatttt tgattattat attcttatgc aatgctaaat ggctcaggct
     1141 atgttttaga ttatcatgtt cttctgcaat gctgaatgcc ccgggtcatt tttttatgct
     1201 cagccctcta atgcctgaac gatggcctgc tcaataccag ccacatcata accgcattca
     1261 tgccagagtt cctgctggga cccgtgctcg acgaagtaat caggtatgcc cagacgtatg
     1321 atctccaccg ggtaaccatg atcatttgca aactccagta cggcgctgcc aaatccgcct
     1381 tttatcatgc cgtcttccac ggttatgatc tttttgaatt gattcatgac gctatgcagc
     1441 acctgctcgt caagcgggtt cacgaaacgc atatcgtagt gcagtatcga gattcccttt
     1501 tcactgagcc gtgcggcagc ttcaacaaca aaatttcccg ggtgaccaag gctcagtacg
     1561 gcaatgtcag atccctccct gataagccgg gcgcgtccaa ccggtatctc cctgaaaggc
     1621 tggtgccagt cagctataac ccccgccccg cggggataac ggatgctgaa gggtccctta
     1681 acagtatcaa gctgggcagt gtacatcatg ttgcgcagtt caacctcatc catgggcgct
     1741 gacgcgatca tccccgggat gttgcgcatg aatgcgatgt caaagaaacc gtggtgcgtt
     1801 gctccatcag ggccgaccag tcctccccgg tccatgcaga atatcacctt gagtctctgg
     1861 agagcgacat catgcaccac ctgatcatag gcacgctgca tgaatgacga gtagatattg
     1921 cagaaaggca gaagcccgtt tgccgccagt cctgccgaga aggtaactgc atgctgctca
     1981 cagataccaa cgtcaaatgc cctgtcaggc atctccttca tcattatgtt gagcgaacaa
     2041 cccgagggca tggcaggggt tattcccact atcttttcat tctgccgggc aagctccaca
     2101 attgtataac cgaatacctc ctggtagagt gagtgtttgg gaccttcggc cggtctgata
     2161 agttcaccgg tgcatttgtc aaatataccg ggggcatgaa agattgtctg ctgttcctcg
     2221 gctttcggga acccttttcc cttcacagtg acacagtgca gcagcttggg cccgggaatg
     2281 gtcttaagat cttcaagtac acgggtaaga tgaagtacgt catgtccgtc aatcggaccg
     2341 aaatatctga atttgaatgc ctcaaacagg ttgctctgtt taaggacagt acccttgatg
     2401 ccgccctcaa gctgtgatgc aaggacccgg gccctgatac caaccctctt gtagcggccg
     2461 agaagtcgcc atatcttatc cttgaatctg ttgtaagtcc tgcttgtggt gatatcaagc
     2521 aggtactcct tcagggcgcc aacactggcg tctattgaga tattattatc attcaggatg
     2581 accagcaggt cagctttctg attgccggcg ttattcaggc cttcgaaagc cagccctgcc
     2641 tgaagggctc cgtcgcctat cactgccacc acctttcgtt tctcgccctt ggcctgggct
     2701 gcaacggcca tccccagcgc tgcggagatg gatgtggagg agtgccccac accgaaagca
     2761 tcgtattcac tttcagacat tcgcgggaaa ccgctgatac ccttgtactt cctgttcgta
     2821 ttgaactgcg tctttctgcc tgtcaatatc ttgtgcccgt aagcctgatg acccacatcc
     2881 cagacaatct tgtcataagg ggtattgtag acatagtgaa gggcaacagt cagttcaacc
     2941 actccgaggc ttgcccccag gtgacccgga ttatggctga cctcatcaat aatgtgctgt
     3001 ctgagctcat tacttagtct taccaggtca agccttttaa gtttcctgag atcgtcaggc
     3061 gtgtttatct tttctaacag ccgttctgaa gatgacattg gttcaggttg tcaagtgccg
     3121 ggccaaagat acttatattt tactttttcc cgaatcggga acagatgcgg caatagggcc
     3181 acctcatgcc tttcgccggt gaaatgcaca atgtttttaa tgacctgatt cagttcgttg
     3241 tctgccatac tcctgttatg gtggtaaaaa ttgaatgttg ttaattaatc atcaatcaat
     3301 gagtaacacc ataccttatt tattgtttat ttagcatcgg atgtaaagca aaaatcaaat
     3361 gattatgaaa gcacccgtaa taaggttgtc atttctgttg ctggctctca tcgcggtctc
     3421 ctgtgttacg gggaagagat acaacactct ccttgaaagg aacctgatgc tgatgtcaga
     3481 gcgtgattcg ctcaaggcgg aaaacaactg gctgacgatg tcaaacaggg aacttacggc
     3541 aaagtcgatg cagcttgcag aagataccga aaggctcacc cgggaggttg agaaagcgcg
     3601 ggcggacctt gcacagtcaa gggatgatgt cagccggctc tccacaaggt acgatgagtt
     3661 gcagagggcc caggccgagc tggttgcagg gaaggcccag gagacgcaga ggctgcttgc
     3721 agagctgagg caggcgcaga cggaacttca gcagagggaa gacctgatgc gtgatcttga
     3781 gatgaacctt gacaccaaaa aagcaaccct tgaggagctc acctatgagc tggaaaagcg
     3841 caatatacgg cttgccgagc tggaaaaaat gcttgacgca cagcagaagg ccgtgaaggc
     3901 gttgaaggac aaggtgtctg aagccctgtt cgggtttgaa aacaacgggc tgacggtttc
     3961 catgaaaaat ggtcaggtat acgtgtcaat ggatgacaag ctgctcttca aaaccggcag
     4021 ctatgagatt gacgccaacg gtcgcacggc actgcgcaag ctcggagcgc tacttgagaa
     4081 gaacactgat gtgaccatca ccattgaggg gcatactgat gatgttccgt acagggcagc
     4141 tccggggcag cagatacttg acaactggga cctgagcgtc aagagggcta caactgtagt
     4201 cagggtgctg atccaggata cacatatcaa cccaaaacgg tttgttgcct cgggacgaag
     4261 cgaatacatc ccggttgacc cggcgaaaac gcctgacgcc cgccagaaaa acaggaggac
     4321 agaaataatt cttacccctg acctgaccgc actttacagg atacttgaga gttattagag
     4381 acctgccccg ggccacagct gagaacacgg tgcggcctgc gaacactgcc aggaactcag
     4441 actggtatga gggcaccacc ggaaatcggg ggaggtctgc tttgggtaac ggcaggaaat
     4501 ccgtgg
//
LOCUS       DAOPBK010000185         4501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_15956_length_4501_cov_4.830634, whole genome shotgun sequence.
ACCESSION   DAOPBK010000185 DAOPBK010000000
VERSION     DAOPBK010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15956_length_4501_cov_4.830634"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..2432)
                     /locus_tag="PLA17_09645"
     CDS             complement(30..2432)
                     /locus_tag="PLA17_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK85715.1"
                     /translation="MNKLTFLLLFLAPLINLGAQTVTVLSADDSKPIADVAVYNEART
                     VFGYTNYSGKVSLSEFTGGEPVFFQHFSYERVSFTIEELEAAQWVVKLETKTFEVEEF
                     IISANRWEQKSEEVPNNINVVALSAVKIMNPQTAADLISMGDVFVQKSQLGGGSPMIR
                     GFATNRVLINIDGVRMNNAIYREGNIQNIISIDPNIIERTEIIFGPGATMYGSDALGG
                     VMDFHTRRALFSTGDSILIKAEAMARWSSASDEQTYHAWINAGGRKVAFLSSVTWSDF
                     GDLKMGSVSHPEYLRDIYQKRVGNRDSVFMNSNPRKQVASGYKQINTTNKLRFKAGKH
                     LDIDLAHHYSRLSDVPRYDRLIQVRSGTLRFGDWYYGPQVWMMTNASVRYNRPTTLFD
                     EIRLTVARQDYRESRHDRALNKTTLNEQFEKVGIWSANLDFDKKSGKRDNLAYYGAEY
                     VWNTIHSTADVKNIVTGETEPAGSRYPNGKNIYNSISLYGGYKFNLSSRFTLSTGARY
                     NYVSLNSEIADNSWYNFPFTSINSGNDALTGAAGAVYRPDDRTELTVNLSTGFRAPNL
                     DDLGKVFESAPGVLVVPNPDLKPEYLYNIDLGASRKFGKTLLAEASLFWSYLDNAMVR
                     REFLFNGQPTFIFQGEESQVYAMVNAGHAIVYGTQLKAELRPSSFIRVKSSLSLIAGH
                     DDEKAPLRHVPPLFGATHVVFERSALKADLYALYNGSITNERMAPSEKDKPYLYARDE
                     NGNPWSPGWFTLNFKTSYNINNRLDLTAGIENLLDLRYRPYSSGISAPGRNFIIAARI
                     KI"
     gene            2592..>4501
                     /locus_tag="PLA17_09650"
     CDS             2592..>4501
                     /locus_tag="PLA17_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85716.1"
                     /translation="MRKRTGIILVVILAIALAVAAFFLRKEKQVVVIDPWEAVPADAF
                     FIIETSDFPELLTRITDPAGILSGLSGLDWAASLVRSASSIDSVTGGREVRELISNRR
                     MLISFHTAGQRQAAPMVVMSTGPAFTARKLMLLCSQAGATVTDKRDLGGTRTFTLTFS
                     QGSLQKSVYLALTSGILIISPSESMITAALDNRSAGSDIRHQQGFSRVVSAAGKTTDN
                     LFVLFRNLPPFLKSFTDPEDISAISSVAIAGGGDLTTAEEGLFISGFLTTAGAGTGAD
                     RLRDVVPAECGVHELLPRNTLSYSTVMRRASLSGETASDPASINATDLALILSPFTGT
                     EVTEGLIASGEGSERIRLFRMSDPQSAEEVLQQRLKARYRYMGLRESHFMASARNSDG
                     TEEVIYRMPFTGVASILAGVEKSKAGDQWVTFARSYMIFSESPEALAAVLRESALGNT
                     LINDPGFREMEKSMPTKSSYLFFSSGRVLKSQLAEYLTPAAVASLSDRALSGISGVAV
                     SMTPSNEMIYTSLSVRYSAGGEHQGSAPAAATDVTTGETDSSGLKLLWKVKLDAEPSV
                     RPFFFTNHNTGATEIFIQDRNNNIYLISSSGKILWKAAIREKITGNIFMIDYYKNGKL
                     QLLCAGRDYMHL"
BASE COUNT         1001 a         1127 c         1237 g         1136 t
ORIGIN      
        1 tttagcattg aatgactggc ggccggggct cagattttga ttctggcggc tatgataaaa
       61 tttcttcccg gcgctgatat gcctgaagag tatggcctgt acctgaggtc aagcaggttc
      121 tctatcccgg ctgtcagatc aagcctgttg ttgatattat aggaggtctt gaagttaagg
      181 gtgaaccatc cgggtgacca cgggttgcca ttttcgtccc tggcataaag atatggcttg
      241 tccttttcgc tgggggccat cctctcattg gtgatgcttc cgttgtagag cgcatagagg
      301 tcggctttca gggccgaacg ttcaaaaacc acgtgcgttg caccgaagag aggcggaaca
      361 tgcctcagcg gagccttctc gtcgtcatgt ccggcaatga ggctgagtga tgacttgact
      421 ctgatgaatg atgagggtcg cagctcggcc ttaagctggg tgccatagac aatggcatgc
      481 ccggcattga ccatggcata gacctggctc tcctcgccct ggaagataaa tgtgggttgg
      541 ccattgaaga ggaactctct tcgtaccatg gcattgtcaa ggtacgacca gaagagcgac
      601 gcttctgcaa ggagtgtttt gccgaacttc ctcgaggcgc caaggtcaat gttatagagg
      661 tattccggct tcagatccgg atttggtact accagcacac cgggggccga ttcaaagacc
      721 ttgcccaggt cgtcaaggtt aggagccctg aatccggtgg agaggttgac cgtcagctcc
      781 gtacggtcgt caggcctgta gacggcaccg gctgcgccag taagggcatc gttgcctgaa
      841 ttgatggatg tgaacggaaa attataccat gaattgtcag ctatctccga gttcaggctg
      901 acatagttgt acctggctcc cgtgctgagg gtgaacctgc tgctgaggtt gaacttgtaa
      961 ccaccgtaga gggagatgct gttatagatg ttttttccgt tgggataacg tgacccggca
     1021 ggctcggtct caccggtaac aatatttttt acatcggcgg tgctgtggat agtgttccat
     1081 acatattctg ccccgtagta tgccaggtta tccctcttgc cgctcttctt gtcgaagtcg
     1141 agattggctg accatatccc tactttttca aactgttcat tgagggttgt tttgttgagt
     1201 gccctgtcat ggcggctctc gcggtaatcc tgtctggcca ctgtaaggcg gatctcatcg
     1261 aagagagttg tgggcctgtt atagcgtacc gatgcgttgg tcatcatcca cacctgcggc
     1321 ccgtaatacc agtcgccgaa cctcagtgtc ccgctcctta cctggatgag cctgtcatag
     1381 cggggaacat cagagaggcg ggagtaatgg tgtgccaggt ctatatccag gtgttttccc
     1441 gccttgaagc ggagcttgtt ggtggtgttg atctgtttgt agccggaagc cacctgtttg
     1501 cgggggttgc tgttcatgaa cacgctgtca cggttgccga cacgcttctg gtatatatcg
     1561 cgaaggtatt cggggtgtga tactgatccc attttcaggt cgccgaagtc agaccatgtc
     1621 actgacgaca ggaaggccac ctttctgccg ccggcgttga tccatgcgtg gtatgtctgt
     1681 tcatcggagg cggatgacca gcgtgccatt gcctcagctt tgatcaatat ggaatcgccg
     1741 gtagagaaga gagcccgccg ggtatggaag tccatgactc cgcccagagc atcactgccg
     1801 tacatggtgg ctcccggtcc gaagatgatc tccgtacgtt ctatgatgtt cggatcaatt
     1861 gaaatgatat tctgaatgtt gccctcacgg tagatggcat tgttcattct cactccgtca
     1921 atattgataa gtaccctgtt ggtggcaaaa ccacgtatca tggggcttcc tcctccaagc
     1981 tggctcttct gtacaaagac gtcacccatg gagatcaggt cagctgctgt ctgcgggttc
     2041 atgatcttta ctgctgagag cgccaccacg ttgatattgt taggcacctc ctccgatttc
     2101 tgttcccatc ggttcgcaga gatgatgaac tcctccacct cgaaggtttt tgtctccagc
     2161 ttcaccaccc actgtgcagc ctcaagctct tcgatggtga atgaaaccct ttcgtatgaa
     2221 aagtgctgga agaaaaccgg ttctcccccg gtgaactccg agaggctcac cttcccggaa
     2281 taattggtat aaccgaacac tgttctggct tcgttgtaaa cagccacgtc agctatgggt
     2341 ttgctgtcat cggcactaag aacggtgaca gtctgggcgc caaggtttat caggggggct
     2401 aaaaagagca gcagaaaggt tagtttattc atctggcaat attgttctca cagcaatttt
     2461 cgcacaaaaa gcattccgtt gctattcatt tgtaatatgt tttccggcat atcaacagtg
     2521 tctcgtcaaa ctcttcgcgg ggcttcctga tatttttata tttttgatgt cagatagcaa
     2581 cagggatatt tgtgcggaag agaacaggca tcatattggt ggtgattctt gccattgctt
     2641 tggctgtggc agcttttttt ctccgcaagg agaagcaggt ggtggtcatt gacccgtggg
     2701 aggcagtgcc tgccgatgct ttctttatca ttgaaacctc tgatttccct gagcttctga
     2761 cccgtattac cgatcctgca ggcatattgt cagggctgtc aggccttgac tgggctgcgt
     2821 cacttgtgcg ctccgcctct tcaattgaca gtgtcaccgg tggcagggaa gttcgtgagc
     2881 tcataagcaa ccggaggatg ctcatctcgt ttcatactgc cggacagcgg caggctgcgc
     2941 caatggtggt gatgagcacc ggacctgcat tcacggcaag gaagctgatg ttgctttgca
     3001 gccaggcggg ggccacggta accgacaagc gcgatcttgg aggcacacgt acattcacac
     3061 ttacattcag tcaggggagc ttacagaagt cagtttatct cgctctcacc tccggcatcc
     3121 tgataatttc tccctccgag agcatgatta ccgcagctct tgacaacagg agcgccggtt
     3181 cagatatacg gcatcagcag ggtttctccc gggtggtcag cgctgccggc aaaactactg
     3241 acaatctctt cgtgttgttc agaaacctcc ctccattcct gaaatcattc acagaccctg
     3301 aagatatctc tgctatttcg tcagtggcaa ttgccggggg cggagatctc accaccgcgg
     3361 aggaggggct tttcataagc gggttcctga ctactgccgg tgcaggtacg ggagcagacc
     3421 gactcaggga cgttgttccc gcagagtgcg gggtgcatga gcttcttcca cgcaataccc
     3481 tcagctacag tacggtgatg cgacgtgcct cactctcggg agaaacggca tctgatccgg
     3541 catcaataaa tgcaacagac cttgccctta ttctgagccc ctttaccggc acagaggtta
     3601 ctgaagggct gatagcatcc ggtgagggca gtgagcggat acgtctcttc cgtatgagtg
     3661 acccgcagtc ggcggaagag gtgctgcaac agcggctgaa agccaggtac cgctatatgg
     3721 ggctaaggga gagtcatttt atggcttcag ccagaaacag tgacgggacg gaggaggtga
     3781 tctaccgtat gccattcacg ggggtggcat ccatacttgc cggggtggag aaaagcaagg
     3841 cgggtgacca gtgggtgaca tttgcaaggt catacatgat cttctccgaa tcgcctgaag
     3901 cactcgcggc tgtgctcagg gagtctgccc tcggaaatac ccttatcaac gacccgggtt
     3961 tcagggagat ggagaaaagc atgcccacaa agagctccta tctctttttc tcatcaggca
     4021 gggtgctgaa gtcacagctg gcagagtatc tcactccggc tgcagttgcc tccctgagtg
     4081 acagggcgct gtcagggatc agcggggtgg cggtgagcat gacgccaagc aatgagatga
     4141 tttacacgag cctctcggta agatacagcg ccggcggtga acaccagggg agcgctccgg
     4201 ctgcagcaac agatgtaaca acaggtgaaa ccgattcatc agggctcaaa ctgctgtgga
     4261 aggtgaagct tgatgcagaa ccttccgtca gaccgttctt cttcaccaac cataacaccg
     4321 gcgcaacgga aatattcata caggaccgca acaacaacat ttaccttata agttcttcag
     4381 gaaagatact ctggaaggca gcaatcaggg aaaagataac cgggaatatc ttcatgatag
     4441 attactacaa gaatggcaag ctgcagcttc tttgtgccgg ccgtgactat atgcacctta
     4501 t
//
LOCUS       DAOPBK010000186         4462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_16114_length_4462_cov_4.436578, whole genome shotgun sequence.
ACCESSION   DAOPBK010000186 DAOPBK010000000
VERSION     DAOPBK010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16114_length_4462_cov_4.436578"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLA17_09655"
     CDS             complement(<1..220)
                     /locus_tag="PLA17_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK85717.1"
                     /translation="MKKIEMVDLWSQYERLKADIDNAIAGVISSTAFINGPDKRLFEE
                     ELQRYLGNRHVIACGNGTDALQIAMMALG"
     gene            324..1649
                     /locus_tag="PLA17_09660"
     CDS             324..1649
                     /locus_tag="PLA17_09660"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPK85718.1"
                     /translation="MARDISELIKELLLKHDCVIIPGFGAFIGNYFPARASRKDGLFE
                     PPSRKITFNRYLTGNDGLLIGHVSAVTGKGYGEARDIVKKWSEELRSKIMTGNPVMIY
                     RLGTFSLNYEGAIVFEPDLTVNYLLSSYGLSAYHRKPVSGFDVRKMAIEKRHTPAVSQ
                     PSMRSLLTRAAVILPLLVALALVPFNDRIFRSNIEESNLNPLARAELEFNREQIDADR
                     SVAEPEINITRDEGNQDNRVNEMKAALGLTVQDSEPVSGPAERAVKTPSAERTVSTAP
                     ADKAVKTAPAERKVRTAAPAESAVKTAQTEAAVKTAPAVESPAEKPASTVKMPEANGA
                     SSVAEPEKEALNATAANRAPARPAGRTTAVVVEDYRYMVIIGSFKGEDNALVMVEKLR
                     QKGYDPEVTGGPDGFLRVSAKALPTLDEAKVVLERLRREYPGAWVFKSR"
     gene            complement(1818..3851)
                     /gene="metG"
                     /locus_tag="PLA17_09665"
     CDS             complement(1818..3851)
                     /gene="metG"
                     /locus_tag="PLA17_09665"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPK85719.1"
                     /translation="MKKYKRHLVTSALPYANGPIHIGHLAGVYVPSDIYTRYLRMRGE
                     DVISICGSDEHGVPITLKARKEGITPQEVVDRYHELNKKAFADFGIAFDIYSRTSNKI
                     HYETASQFFRKLYDKGEFVEKTTSQYYDEEAGCFLADRYITGTCPHCGNEEAFGDQCE
                     KCGTSLSPTDLINPKSTISGSAPVMRDTLHWYLPLDKHEPWIRKWILEEHTEWKPNVY
                     GQCKSWLDQGLQPRAVSRDLDWGVPVPLGNAKGKVLYVWFDAPIGYISATRELTPDWE
                     KYWKDPETRMVHFIGKDNIVFHCIIFPAMLKAEGSYILPDNVPANEFLNLENDKISTS
                     KNWAVWLHEYLEDFPGKQDSLRYALCASAPETKDNDFTWKEFQARNNNELVAILGNFV
                     NRTLVLTANYYNGTVPVPGVMNDYDRETVKSIMTCRENTESNLEAFRFRQALAEAMNL
                     ARLGNKYLADTEPWKVIKTDPERVKTIMYISLQITANLAILLEPFLPFSMEKLRGWLG
                     IAGLQWGAAGRSDLLSPGHGISSGELLFSKIDDAEIEKQIQKLLDTKKANHNAKAPVL
                     PQKEDVTFDEFTRMDIRTATILEADKVPKTTKLIKLKVDTGIDVRTIVSGIAEHFTAE
                     ELPGKNISVVVNLAPRTIRGIESKGMVLLAEDENGKLVFVTPETKISNGSPVK"
     gene            complement(3920..>4462)
                     /locus_tag="PLA17_09670"
     CDS             complement(3920..>4462)
                     /locus_tag="PLA17_09670"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPK85720.1"
                     /translation="FFTLFEIVILYDQRPFPELLVRSVQVTSLTLLLPYTFLWLWFAW
                     REGKKTLSGIMEQPETASRHPMIHLRDEKGVLRLSLKHDNLLYMQASDNYVTVFYQAQ
                     DKLTRFMLRNTLRALEEEFKEEAIIRCHRSWLVNIEKVKLIRREKDGLLLELDSTPPS
                     SVPVSRTYMHDVLAAFGHVE"
BASE COUNT         1036 a         1113 c         1207 g         1106 t
ORIGIN      
        1 gtcccagcgc catcattgct atctggagag catcggtgcc attgccacag gcgatgacat
       61 gccggttgcc aaggtaacgt tgcaactcct cctcgaagag acgtttgtca ggtccattaa
      121 tgaaggcagt ggaactgatc acaccggcta ttgcattatc aatgtcggcc ttaagcctct
      181 catactgtga ccacaggtca accatctcta tctttttcat cttctgaact ctatcttgat
      241 agcgaagata atagatttta attaacccat tttcctgagc cggatcaggt ttcttttata
      301 tctttgttta atatttgata tgcatggcga gggatatcag tgaacttatc aaggaacttc
      361 tcttaaagca cgactgcgtc attataccgg ggtttggtgc gttcatcggg aattattttc
      421 cggctcgtgc aagccggaaa gatggtctct ttgagcctcc ttcacggaag atcaccttca
      481 accgttacct taccgggaat gacggactgc tgataggtca tgtctctgcc gtgacaggaa
      541 aaggatatgg tgaggcacgg gatattgtaa aaaagtggtc agaggagctg agaagcaaaa
      601 tcatgaccgg caacccggtg atgatttacc ggctgggaac cttttcgctg aattatgagg
      661 gggcaatagt atttgaacct gaccttacgg ttaattacct cctttcgtca tacggactca
      721 gcgcctatca ccggaaaccg gtgagtggct ttgatgtcag gaagatggct attgaaaagc
      781 gccatacccc tgccgtcagc cagccttcca tgagaagcct gctgacaagg gccgctgtga
      841 tactaccgct gctggtggct cttgccctgg ttcctttcaa tgaccggata ttcaggagca
      901 atattgagga atcaaacctc aatcccctcg ccagggctga acttgagttc aaccgtgaac
      961 agattgatgc tgacaggtca gttgcagagc ctgaaatcaa tatcacccgt gatgagggca
     1021 atcaggataa cagagtcaat gaaatgaaag ccgctttagg actgaccgtt caggattcag
     1081 aacctgtttc cggcccggcg gaaagagcag ttaaaactcc ttcggcagag aggacagtca
     1141 gcactgcccc tgcagataaa gcagtcaaaa ctgccccggc agaaaggaaa gtcagaactg
     1201 ctgctccggc agaaagtgcg gtcaaaactg cccagacaga ggcagcggtc aaaaccgctc
     1261 cggcggttga atccccggct gaaaaacctg cttccaccgt taagatgcct gaagcgaatg
     1321 gtgcatcatc tgttgcagag ccggagaagg aagcccttaa tgccaccgcg gcaaacaggg
     1381 ctcctgcgag gcctgcgggg agaacaaccg cggttgtggt ggaggattac aggtacatgg
     1441 tcattatcgg cagtttcaag ggtgaggata atgctcttgt catggttgaa aaactccgcc
     1501 agaaaggata tgacccggag gtgacaggcg gaccggacgg gttcctcagg gtcagcgcaa
     1561 aggcactccc cacactcgat gaggcaaagg ttgttctcga aaggctgcgc cgggagtatc
     1621 cgggagcgtg ggtgttcaaa tcgaggtagc tgacagtatc agccggcagc cccggtcagc
     1681 aatgctagtc tgcatcaatc agcttgccag cggcattcat cagtagtgcc agtcagtagt
     1741 cagcagaatc agtcagcagc ttcggtcttc agcagccggc agccccggtc cttagtagtt
     1801 tcgcaaatta ttccctgcta ttttacggga cttccgttgc tgatttttgt ctcgggggtg
     1861 acaaatacca gtttgccgtt ttcgtcctcg gcaagcagta ccattccttt tgactcgatg
     1921 ccccttatgg tacgcggcgc caggttgaca accacggata tattctttcc gggcagctcc
     1981 tcagccgtga aatgttcggc aatacctgaa acgatggtac ggacatcaat gccggtatca
     2041 accttcagtt tgatgagctt tgtggttttg ggcaccttat cagcctcaag tattgttgct
     2101 gtgcggatat ccatccgggt gaactcgtca aaggtgacat cctctttctg aggtaggacg
     2161 ggagccttgg cgttatggtt tgcttttttg gtatcaagaa gcttttgaat ctgtttttcg
     2221 atctctgcat catctatctt gctgaacagc agctcgcccg atgatatgcc atggcctgga
     2281 gagagaagat ctgaacggcc ggcggcaccc cactgcagcc ctgcgatgcc cagccatcct
     2341 ctcagcttct ccattgagaa ggggaggaac ggctcaagca gaattgccag gttggctgtg
     2401 atctgcaggg agatatacat gatggtctta acccgttcag ggtctgtctt gatcactttc
     2461 cacggttcgg tgtcagcaag gtacttgttc ccaaggcgcg cgaggttcat cgcctcggca
     2521 agcgcctggc ggaacctgaa ggcctcaagg ttgctctcag tgttctcacg gcaggtcatg
     2581 atcgacttca cagtctccct gtcataatca ttcatgacgc ccggaacggg aacggtaccg
     2641 ttgtagtagt ttgccgtcag tactagtgtg cggttgacga agttgccgag gatggcaacg
     2701 agttcgttat tattgcgggc ctggaactcc ttccaggtga agtcgttgtc cttggtctcg
     2761 ggtgcgcttg cacagagcgc gtagcgaagg gaatcctgct tgccgggaaa atcctcaaga
     2821 tattcatgca gccacacagc ccagttcttc gaagtggaga tcttgtcatt ctcgagattc
     2881 aggaactcgt tggccggcac attgtcggga aggatatagc tcccctctgc cttaagcatg
     2941 gcagggaaga tgatgcaatg gaacacgata ttgtcctttc ctataaagtg caccattcgg
     3001 gtctccgggt ctttccagta cttctcccag tcaggggtca gctccctggt tgcggagata
     3061 tacccgatgg gggcgtcgaa ccagacgtac agcactttac ccttagcgtt cccgaggggt
     3121 acgggcactc cccagtcaag gtcacgactg acagccctgg gctgaagccc ctggtcaagc
     3181 catgacttgc attgtccgta tacatttggt ttccattcgg tatgttcttc gaggatccac
     3241 ttcctgatcc agggctcatg tttgtcaagc ggaaggtacc agtggagtgt gtcacgcatc
     3301 accggggcac tgccgctgat ggttgattta gggttgataa ggtcagtggg gctgagtgat
     3361 gttccgcact tctcgcactg gtcaccgaag gcctcctcat tgccgcagtg agggcaggta
     3421 ccggttatat acctgtcagc caggaagcaa ccggcctctt cgtcataata ctggctggtg
     3481 gttttctcaa cgaattcccc cttgtcatac agtttgcgga aaaactgcga agcggtttca
     3541 tagtgaatct tgttcgatgt gcgggagtag atgtcaaagg cgataccgaa gtcggcaaaa
     3601 gccttcttgt tcagctcatg gtagcggtca accacctcct gcggagtgat tccctccttg
     3661 cgggctttca gggtgatggg tactccatgc tcgtcggagc cgcagattga aatgacatcc
     3721 tcgccgcgca tccggaggta acgggtatag atatccgacg gtacgtaaac ccctgcaagg
     3781 tgccctatgt gtattggtcc gtttgcatac ggaagggctg atgtgaccag atggcgcttg
     3841 tatttcttca tttgaaggag gtgattgctg attttaaccc ggcaaagata atgattttca
     3901 gaaaacgccg ggagacaggc tattcaacat gcccgaaggc tgcgagcaca tcgtgcatgt
     3961 atgtgcgtga caccggtacc gatgatggcg gagtgctgtc gagctcaagg agaagtccat
     4021 ccttctccct gcgtatcagc ttgacttttt caatatttac aagccatgag cggtggcatc
     4081 ggattatagc ctcctcctta aactcctcct ccagagccct gagggtattt cgcagcatga
     4141 acctggtcag cttatcctga gcctggtaaa agacagtgac gtaattgtcg cttgcctgca
     4201 tatagagaag gttgtcatgc ttcagtgaaa gcctcagcac tcccttttcg tcacggaggt
     4261 gtatcatcgg gtgccgggaa gccgtctcgg gctgctccat aatccctgac agagttttct
     4321 tgccttcgcg ccatgcgaac catagccaca gaaaggtata gggaagaagc agggtgagcg
     4381 aagtgacctg aactgatctt acaaggagct caggaaaggg gcgctggtca taaaggatga
     4441 ctatctcgaa aagggtgaag aa
//
LOCUS       DAOPBK010000187         4452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_16162_length_4452_cov_4.215147, whole genome shotgun sequence.
ACCESSION   DAOPBK010000187 DAOPBK010000000
VERSION     DAOPBK010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16162_length_4452_cov_4.215147"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLA17_09675"
     CDS             complement(<1..1010)
                     /locus_tag="PLA17_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK85721.1"
                     /translation="MKINRRNFLRTATVAGAGTLTAVSAASCVRREKSTEAMAEIRAA
                     VMAVHNQSFNMSGYAAPPLKKVRTGFIGLGMRGPGAVERMAHIEGVEIASLCDKYPDR
                     VEKVQAMLTKFGLPQAKDFSGSEEAWKGLCDDPDIDLVYICTPWAWHTPMAVYAMEAG
                     KHVAVEVPAAKTVEECWQLVEVSERTKKHCMQLENCCYDFFELLTLNMARQGLFGDIV
                     HGEGAYIHDLRWDYTYNKNGYADMWRLKENVRNGNLYPTHGLGPVCQIMNINRGDRME
                     YLTSLSSNDFVMGPEVEQLAGEDQFYAPFAGKSYRGNMNTTLIKTALGRSIMIQHDVS
                     SL"
     gene            complement(1220..1453)
                     /locus_tag="PLA17_09680"
     CDS             complement(1220..1453)
                     /locus_tag="PLA17_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85722.1"
                     /translation="MKKDFLRKYLPEIICTVAGAIGGFLYWKYVGCVSGTCTIKSNWY
                     LMIPWAAVLGYLVGSVSGDLIRKRRRPPEQEAH"
     gene            complement(1450..2586)
                     /locus_tag="PLA17_09685"
     CDS             complement(1450..2586)
                     /locus_tag="PLA17_09685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK85723.1"
                     /translation="MRVIIAVISDLSTDMRVQKHSALLSAMGFSVTLIGRHNPNTLPL
                     SLPSATVSVLRVPFRKGPMMYLWFNIALLFRLLRLRKADIYIANDLDTLIPCYMVSRL
                     NRKALVYDAHEYFTGQYSLTERRFKRSLWKRAERSLLPGIRYMMTVSDSIAKIYRQEY
                     GCEPVVVRNLPMPAFNIPPYQRSHLTAAADDLLVVFQDGSGDNPGRGVSELLEAMTLL
                     ENVKLVVIGTGKRIDEARQQVEKQRIDHKIIFLPRMKWEEMIGYIKCCDAGLSIDADT
                     CINQRFSLPNKLFDTIGAGVPVIVSPLPEVSAIIAKYRCGLVLDEMTPGAIACALSLL
                     RDDRQLLSELKVNAHSAAEELNWEKEQTAEQDLFGRVIKENNIK"
     gene            2689..3837
                     /locus_tag="PLA17_09690"
     CDS             2689..3837
                     /locus_tag="PLA17_09690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK85724.1"
                     /translation="MESASSKRIVHLVSFNIPYPPDYGGIMDVFYKISALKRQDVGVI
                     LHCFSYGRGRSRTLERECLKVHYYRRELNLFHIFRKEPFIVVSRRNEMLLRNLLTDIH
                     PIIFEGLHTTWFLCHKALEGRIKMVRTHNIEHVYYRNLALNEKNPLRRLYFNTEARKL
                     ERYEPKLAGANSLLSISPGDTEYFRSKYGNAVFVGPFHPAEVSFPDNGRGDYVLMHGD
                     FSTAENNAAALHILKEVVSHWKYRTVIAGKRPSAELSDAASSLRHVKLVPNPSMTQMN
                     ELIANAQVCLLNASNPTGMKLKLINALTFGRHVVASPAIVSGTGLEELCHKAISPGEW
                     ISLTGSLMKEDFTSDMRARRNLLLKEIADNDNNAKRIVEAIKSYIPEF"
     gene            complement(4132..>4452)
                     /locus_tag="PLA17_09695"
     CDS             complement(4132..>4452)
                     /locus_tag="PLA17_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPK85725.1"
                     /translation="LGMPLQADPTVKFAVNDFTLRRVLNKHVAVDSPYNTYKFAGLPP
                     GPIRCPSRSGLDAVLNAEKHEYLFFVAKYDGSGYHHFSRTLAEHNRYAADYRRELNRR
                     RIYK"
BASE COUNT         1107 a         1137 c         1004 g         1204 t
ORIGIN      
        1 ctcagactcg aaacgtcatg ctggatcatg atgctccttc cgagcgctgt ctttatcagg
       61 gttgtgttca tgttgccgcg gtaactcttc ccggcaaaag gagcataaaa ctggtcctcg
      121 ccggccagtt gctccacctc cggtcccatg acgaaatcat ttgatgagag tgatgtcagg
      181 tattccatcc tgtcacccct gttgatattc attatctgac agaccggtcc cagaccatgc
      241 gtcgggtaaa ggtttccgtt acgcacattt tccttaagcc tccacatgtc ggcatatccg
      301 ttcttgttat aggtgtagtc ccatctgaga tcatggatat aagccccttc cccatgcact
      361 atgtcgccaa agagtccctg ccgtgccatg ttaagtgtca gcagctcgaa aaaatcatag
      421 cagcagtttt caagctgcat gcagtgtttt tttgtccgct cagatacctc aacaagctgc
      481 cagcactcct caacggtctt agcggcgggt acttccactg ctacatgctt gcctgcctcc
      541 atggcataga cagccatagg ggtgtgccat gcccagggtg tgcatatgta aacaaggtca
      601 atgtccgggt catcacacaa tcccttccag gcttcctcgc ttccactgaa gtcctttgcc
      661 tgcggcagtc cgaactttgt gagcattgcc tgcacctttt caaccctgtc ggggtactta
      721 tcgcagagcg aagctatttc caccccttct atatgagcca tccgctcgac ggcacccgga
      781 cccctcatcc cgagaccgat gaaccctgtc cttacttttt taagtggagg agccgcatag
      841 cctgacatgt tgaagctctg gttatgtacg gccattactg ccgccctgat ctctgccatg
      901 gcctcggttg acttttcgcg acggacacat gaggctgcac tgacagccgt cagggtgcct
      961 gctccggcaa ctgtggcagt gcggagaaaa ttccttctgt tgattttcat ggtttgtagg
     1021 gtaagtttcg aatagtatta tgtatgcaat atacaaagtt tactattata atataatgca
     1081 cgtgattgat tgtaatatga cctttagggt gcgtccggac cgggcgcagg ggaattgccc
     1141 ggccagacat tgatcagtga ccctcatgtt ctttcaaacg cattggcttc accttcatgt
     1201 tataatggcc tgaaatctgt cagtgagctt cctgttccgg cggtctcctt ctcttcctga
     1261 tcaaatcacc tgacacagag ccgaccagat aaccaagcac cgcagcccac ggtatcatca
     1321 ggtaccagtt cgacttgatg gtacatgttc cggatacgca tccgacatat ttccagtaca
     1381 gaaatcctcc tattgcccct gcaaccgtgc agattatttc cggaagatat tttctgagaa
     1441 aatccttttt cattttatgt tgttttcttt aataaccctt ccgaagaggt cttgttcagc
     1501 tgtttgttcc ttttcccagt taagctcttc tgcagcactg tgtgcattga ccttcagttc
     1561 tgaaagcaac tgcctgtcat ccctgagtaa tgaaagtgcg caggcaattg ccccgggcgt
     1621 catttcatca agcacgaggc cacatctgta cttcgctatt attgctgaca cttcaggcag
     1681 cggggaaacg atcacaggta cccctgcacc tatggtatca aatagtttat tggggagact
     1741 gaagcgctga ttgatacagg tgtctgcgtc aatcgataag cctgcatcac agcactttat
     1801 ataaccgatc atctcttccc atttcattct tggcaggaag atgatcttgt gatcaatgcg
     1861 ctgtttttct acctgttgcc tggcctcgtc aattctttta ccggtgccaa tgacaacaag
     1921 cttcacattt tcaagcagcg tcattgcttc aagcagttct gacacacccc tgccaggatt
     1981 atcacctgag ccatcctgaa ataccacaag cagatcatct gccgctgcag tcagatggct
     2041 gcgctggtat ggcgggatat tgaatgcggg catgggaagg tttctgacaa caaccggttc
     2101 acatccgtac tcctgcctgt aaatttttgc aattgaatca gataccgtca tcatgtaacg
     2161 tatcccgggc agaaggctgc gctcagctct cttccagaga gagcgtttga atctcctctc
     2221 agtgaggctg tattgcccgg taaaatattc atgtgcatcg taaacaagag ctttcctgtt
     2281 taaacgtgaa accatataac atggaatcag tgtgtcaaga tcattggcaa tatatatatc
     2341 tgctttcctc aaccgtaaca gcctgaacaa taacgctata ttgaaccata aatacatcat
     2401 cggacccttc ctgaaaggca ccctgagcac tgacacggtt gccgaaggaa gagagagagg
     2461 caaagtgttt gggttatgcc tcccgatgag cgtcacgctg aagcccatcg ctgaaagcag
     2521 agctgaatgc ttctgaaccc tcatatccgt tgacagatca ctgataactg caattattac
     2581 cctcataatc tgcctattac acaaaataac atttatacta ttgctgttaa aaccggcata
     2641 agatacaaag aaaattatag ctatttttgc atattgtatt aatgcttaat ggagagcgct
     2701 tccagcaaaa gaatagtcca tcttgtgagt ttcaacatcc cttaccctcc tgattacgga
     2761 ggcataatgg atgttttcta caagatttct gcccttaaga ggcaggatgt aggagtaata
     2821 ctccactgtt tcagctatgg gcgcggccgt tcaagaacgc ttgaacgcga atgtctgaag
     2881 gtacattact acaggagaga gctgaatctc tttcatatct ttcgcaaaga gccgttcatt
     2941 gttgtatcac gcaggaacga gatgctcctc aggaacctcc tgactgacat ccacccgata
     3001 attttcgaag ggctgcatac cacctggttc ctgtgtcaca aggcattgga aggaaggatc
     3061 aaaatggtgc ggacacataa cattgagcat gtctactacc gcaacctggc cctgaatgag
     3121 aaaaatcccc tgaggagact ctatttcaac actgaagcca ggaagcttga gcggtacgaa
     3181 ccgaagcttg caggggcaaa ctccctgctc tcgatctctc cgggcgacac agagtatttc
     3241 aggtcaaagt atggcaatgc cgtttttgtc ggtccattcc atccggcaga ggtatcattt
     3301 cctgacaacg gcaggggtga ttatgtactg atgcatggcg atttctccac tgccgagaat
     3361 aatgcagctg cactccatat ccttaaggag gtggtgtctc actggaaata ccggactgtc
     3421 attgcgggca agaggccgtc ggcagaactg tcggatgccg cctcttcgct caggcatgta
     3481 aaactggttc caaacccttc aatgacacag atgaatgaac tgatcgcaaa tgctcaggta
     3541 tgcctgctaa atgcctccaa ccctaccggc atgaaactga agctcatcaa tgcgctcact
     3601 tttggccgtc atgttgtagc ctcaccagca attgtatcag ggacaggact tgaagaactc
     3661 tgccacaagg ccatcagtcc cggggagtgg atcagcctta ccggcagtct gatgaaagag
     3721 gattttacca gtgacatgag agcccggcgc aacctccttc tgaaggaaat agctgacaat
     3781 gacaacaatg caaaaagaat agtggaggca ataaaaagtt acattcctga attttagagg
     3841 cagttttcca aaccgtgttt caggcttggt cttgaaaatt ccaaagactc acagatttgg
     3901 aaattttcca tggagaatgg atatgccatt gtaaccttcg acagcgattt ttatgatctt
     3961 ggacttctca aaggggcttc acacaaggta atatggttga gatttcgtat ctaatcacag
     4021 gggcttattc cgttttcacc gtgccttctt cctgtttgtt tttccgggca ctctctgagt
     4081 gatgttgcct gtcagccggc cggtcatccg gccacagtaa caaccgtctc cctacttgta
     4141 tatccttctc cggttcagtt cacgcctgta gtctgcggca tacctgttgt gctcagccag
     4201 cgttctgcta aaatggtgat aacctgatcc gtcatacttt gcaacaaaga agagatactc
     4261 atgcttttcc gcattcaaaa cggcatcgag acctgagcgt gacggacacc ttattggtcc
     4321 tggcggcagg ccggcaaact tatatgtgtt atagggtgag tcaactgcca cgtgcttgtt
     4381 gagaacacgt ctcagggtga agtcgttgac tgcaaacttc actgtgggat cagcctgaag
     4441 cggcatcccg ag
//
LOCUS       DAOPBK010000188         4394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_16421_length_4394_cov_5.592763, whole genome shotgun sequence.
ACCESSION   DAOPBK010000188 DAOPBK010000000
VERSION     DAOPBK010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16421_length_4394_cov_5.592763"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..85)
                     /locus_tag="PLA17_09700"
     tRNA            complement(10..85)
                     /locus_tag="PLA17_09700"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Lys,seq:ctt)
     gene            complement(198..977)
                     /locus_tag="PLA17_09705"
     CDS             complement(198..977)
                     /locus_tag="PLA17_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85726.1"
                     /translation="MNRERKKWLIIAASLLAVFIIYYLVMSVLAVSRAVNEFDDAYFA
                     LLEQKEGDTIDLCAIPGYIDMIRNKAFLNSQVKLAESDSVGLLINVRDSVIQLLIKGL
                     PVRTVKIDEYDISPFFHSANQEAIYKMLSTPLTVTGMQATFMKDPVSVKIAPRDTSQV
                     VESEKPDTTDFEAVFFTLDTDRNIRFYFEQQEDTIGADRRARFVFDLKDRMRNASVTM
                     RAVARMKIPPYVPYIKIWLPKAEAKIIYRAIPREGMIVLTQ"
     gene            complement(992..1765)
                     /locus_tag="PLA17_09710"
     CDS             complement(992..1765)
                     /locus_tag="PLA17_09710"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPK85727.1"
                     /translation="MEEQTENLPEQAAESSPPAFNKRFNQAVNFLKSDPRARRTLISL
                     AIVLLLLFIFSVPVYIAPAMQDSWGKNRAECRPDSSLLDNRSIRRQMSAAHNEITRLE
                     RRLASFVPTQTYLVINTVDNRFSLYRNRQLVREGYCSSGSYTLLKTEEGDKEWIFKTP
                     RGRLSILGKITNPVWRKPDWAFVEEGLPIPGASDPSRYEFGVLGKYAMSLGDGYLIHG
                     TLYQRQLGMPVTHGCIRLGDEDLEVVFKSLSVGSRVYIY"
     gene            complement(1768..2895)
                     /locus_tag="PLA17_09715"
     CDS             complement(1768..2895)
                     /locus_tag="PLA17_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPK85728.1"
                     /translation="MKRVSLTHIIIYAIAGIVLAVIGVLIFRALVPEPPLPEVEQARV
                     AIVRARDLQSEIYSPKLYREAKNNYDSAMAVWRSENDRFILARDYERVIRFAETARKK
                     AEEASRNTISRSKSLKSTLESEIARLNHEMAAFEKIFLSMPLPQDIKKKHARGKLLIK
                     EAGIDYKKERYVDGNVKITEANEYISSTYNLARKKLEEYFRHYDTWQEWADETIAESR
                     RSGSYAMLVEKIPAQLHLYQGGKKKYTFEAEFGSNWLGDKKSRGDMATPEGKYAVTKK
                     LSGGSTKYHKALLINYPNKIDVQEFNERIRNGQLPADASIGDMIEIHGDGGKGGHWTQ
                     GCVALKNSDMDMLFKYVSKGTPVTIIGSTLTLEEFFSSRVK"
     gene            complement(3007..3606)
                     /locus_tag="PLA17_09720"
     CDS             complement(3007..3606)
                     /locus_tag="PLA17_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85729.1"
                     /translation="MKNKLFLAIATVAVAALMASCAKVPQEALDAAKAALETAKTAQT
                     DVYFPAEFAALNDSLTVMLQNIETQKSKSAKDFKAIKVAADGIALKAAELSENVEARK
                     AEVKAEAETLMADAKTLLDEAKALLVKAPKGKEGKAVVEEMKNELTVIETSLTDAQTL
                     FDEGTNYMEVVDKVKASTEGINGIIAELKAAMEKAGIKF"
BASE COUNT         1001 a         1141 c         1019 g         1233 t
ORIGIN      
        1 cctccctcct ggtcggtctg cagggactcg aaccctggac ccgctgatta agagtcagct
       61 gctctaccac ctgagctaca gaccggaatt aaaacggtgc aaaagtacac tttttcggga
      121 attgtgcatc aaaaaaaatt gcatacctga ttattgattc actattgtgc cggaacaatc
      181 tcccttatgc aactatgcta ttgtgtcagt actatcattc cctcccgcgg aatagcccgg
      241 taaataatct tcgcctcggc ttttgggagc catatcttga tatatggtac atacggtggt
      301 atcttcatcc tcgcaacagc cctcatcgtc acggaagcat tccgcatcct gtccttcagg
      361 tcaaaaacga acctcgcccg gcggtcagct ccaatggtgt cttcctgttg ctcaaagtag
      421 aaccggatat tacggtcagt gtcaagcgtg aaaaataccg cctcaaaatc agtggtgtca
      481 ggcttttcac tctcaaccac ctgcgacgta tccctgggcg caatcttcac actcaccgga
      541 tccttcataa aggtggcctg catgccggtt acggtcagcg gtgtggaaag cattttgtaa
      601 atggcctctt ggtttgcact atgaaaaaat ggcgagatat catactcatc aattttaacc
      661 gtcctcaccg gcaatccttt aatcagcagc tgaatcacgc tgtcgcgaac attgatcagc
      721 aaccccaccg agtccgactc cgccagcttc acctgagaat tcaggaatgc cttgtttcgt
      781 atcatatcaa tgtatccggg gatggcacac agatctatcg tgtctccctc cttctgctcc
      841 agcaaagcga aatacgcatc gtcaaactca ttcacggcac ggctgacagc caaaaccgac
      901 atcaccagat aatagatgat gaaaactgcc agcagagaag cggcaattat cagccatttt
      961 tttctttcgc ggttcatcgc ttcagttctt gtcagtaaat ataaactctt gatcccacgc
     1021 tcagcgattt gaacacaacc tcgaggtcct catcgccaag cctgatgcat ccgtgcgtca
     1081 cgggcatccc gagctgcctc tgatagagtg tcccatgaat aaggtaaccg tcgccgaggc
     1141 tcatggcata cttgcccagc acgccaaact catacctcga cggatcactg gccccgggta
     1201 tgggcagacc ctcctccaca aaagcccagt caggcttacg ccataccgga ttggttatct
     1261 ttcccaggat actcagtctt ccacgcggag tcttgaaaat ccattcctta tcgccctctt
     1321 cagtcttaag gagcgtgtaa ctgcctgatg aacagtatcc ttcacgcacc agctgcctgt
     1381 tcctgtagag agaaaaccgg ttgtcaaccg tatttatcac gaggtaggtc tgtgtgggca
     1441 caaaagaagc aagccttctt tcaaggcgtg ttatctcatt gtgggcggcc gacatctgcc
     1501 tcctgatgga tctgttgtca agcaatgacg agtcaggcct gcactcagcg cgattctttc
     1561 cccacgagtc ctgcatcgca ggagcaatat aaaccggcac ggaaaagatg aacagcaaca
     1621 gcagcactat tgccagggag atcagtgttc gccgtgcccg ggggtcactc ttcaggaaat
     1681 taaccgcctg gttaaacctt ttattaaatg ccggggggct gctttctgca gcttgttccg
     1741 gtaaattttc agtctgctct tccatgatca ctttactcgc gaggagaaaa actcctcaag
     1801 ggttaatgta gaaccgatga tggtcaccgg cgttcctttc gaaacatatt tgaataacat
     1861 gtccatgtca gagtttttca gggcgacgca tccctgtgtc cagtggccac ccttgccgcc
     1921 gtcgccgtga atctcaatca tgtcaccgat gctggcatcg gccgggagct gtccgtttcg
     1981 tatcctctca ttgaattcct gcacgtcaat cttgttcgga tagttgatta acagggcttt
     2041 atgatatttt gtcgatcctc ctgaaagctt cttcgtaaca gcgtattttc cctccggtgt
     2101 agccatgtct cccctgcttt tcttgtcacc cagccagttg cttccgaatt ctgcctcaaa
     2161 ggtatacttc tttttgcctc cctggtacag gtgaagctgc gccggaatct tttccaccag
     2221 catcgcgtat gacccgctcc tcctgctctc ggcaattgtc tcatcagccc actcctgcca
     2281 ggtgtcatag tgcctgaaat attcctccag tttttttctg gcaagattgt aagtgcttga
     2341 aatatactcg ttagcttcgg taattttgac gtttccgtca acgtaccgct cttttttata
     2401 gtcgattccc gcctccttga tgaggagttt tccgcgggcg tgtttctttt tgatatcctg
     2461 cggcaggggc atcgagagga agatcttttc gaatgcagcc atctcgtggt taaggcgggc
     2521 tatttcgctc tcgagggtgc tcttgaggct cttcgatctg gatatggtgt ttcgcgaggc
     2581 ctcttcggcc ttcttccgag ctgtctccgc aaaccgtatg acccgctcgt aatcgcgtgc
     2641 cagtatgaag cgatcgtttt cgcttcgcca tacggccatt gccgagtcgt aattgttctt
     2701 cgcctcgcga taaagtttag gtgaatatat ctctgactgc agatccctgg ctctgacgat
     2761 ggcgacacgt gcctgctcca cctctggcag cggcggctcg ggcacaagag cgcggaaaat
     2821 cagcactccg atgacggcaa gcacgatgcc ggcgatagca taaattataa tatgagtcag
     2881 ggatactctt ttcatgggtg cgaagttaaa aaaatcccag tgaacaggcc cggggattga
     2941 caaaaagaaa agggtgcccc atttgtaata tgagacaccc tcctctttat tggttcaaca
     3001 gtatgcttag aatttgatac cggccttttc cattgctgcc ttcagctcgg cgatgatgcc
     3061 gttgatgcct tcggttgaag cttttacctt atcaacaact tccatgtagt tggtgccttc
     3121 atcgaagagg gtctgagcat cggtgagaga agtctcgatg acggtcagtt cgttcttcat
     3181 ttcttcaaca actgctttgc cttccttgcc tttgggagcc ttgacaagga gagccttagc
     3241 ctcgtcgagg agtgttttgg cgtctgccat cagggtttca gcttcagcct tgacttcagc
     3301 tttcctggct tctacatttt ctgacagttc agctgccttg agagcaatac catcagcggc
     3361 gaccttgata gctttgaaat cttttgctga ctttgacttc tgtgtttcga tgttctggag
     3421 catgacggtg agtgagtcat tcagagcagc gaattcagcc ggaaagtaaa catcagtctg
     3481 ggctgttttt gctgtttcga gagcagcttt tgcagcgtca agagcttcct ggggaacttt
     3541 agcgcagctt gccatcaggg cagctacagc aacggttgcg attgctaaga aaagtttgtt
     3601 tttcatttct gtttaagatt tagttctgtt ataatgttta aaaatatctc cggtttgaaa
     3661 ttgttaaacc gctgcaaagt taaataataa ttttaatctg gttttaatct cgagcgattt
     3721 tttttaatgg aattttaatc tcttttgaat gagtttgtct aaatggctct gtttgagggt
     3781 ttccgcccac agggcgaagc tcttgttttg ttgcttatta tttattgcag cgagattttc
     3841 tggttttttc cggtctgcag actggcgaat ctaatgtttt acagcagggt taagcactcc
     3901 ggaaagatca tatctgcgcc cggcgaatca gaattaaaag ccccctcccg atctgacagc
     3961 atccggatca tcttaggccg cgattgtttt tactgtctaa atcaattccc ggaaactgct
     4021 caattcagtc gcacacactg ttgccgtgcc ggcgaggtgt gagactgcgg cagttcttac
     4081 tatctaaatc aatacccggg aactgcttaa ttcagtcaca cacactgttg ccatgccggc
     4141 gatgtgtgag actgcggcag ttcttactat ctaaatcaat acccgggaac tgctcaattc
     4201 agtcgcacac actgttgccg tgccggcgag gtggcggaac tggtattgac agaggaggaa
     4261 gaaaaggagg aagccccttc agattatccc gggcgtataa agaagccgga ccattttcag
     4321 tgatccggcc tgccttgtct tctgcggaga gagggggatt cgaacccccg gtacggtcac
     4381 ccgtacggca gttt
//
LOCUS       DAOPBK010000189         4338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_16664_length_4338_cov_4.515293, whole genome shotgun sequence.
ACCESSION   DAOPBK010000189 DAOPBK010000000
VERSION     DAOPBK010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16664_length_4338_cov_4.515293"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="PLA17_09725"
     CDS             <1..1691
                     /locus_tag="PLA17_09725"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPK85730.1"
                     /translation="ATAGAQSIVPAEWKFLTGDKPEYIDSAKNEYWWHDISPLTVWEK
                     QGFAGYDGFAWYRVKVVVPVSMKKNAQKYDGLILNLGKIDDADETFFNGHKVGATGKM
                     PPDYSGAYDVPRSYLIPPDYVNWGGKNTIAVRVYDAGGDGGLYGGPVELTNRGNPDLL
                     TLGAVAAQEDMILKGSPDVEILATVKSEFRKMYRGALTLEIVTDFGDQVYDGYREVAV
                     KKMSTGSFPFLVENLKPGFYKATLALESKMGNKKYNFNLGYEPEKIVSPADPQPDFTE
                     FWQRAKAELATVDPRYNLIRIDSLCTEKHDVYLVEMRSLGNALIRGWYRVPVKPGRYP
                     AIMQLPGYSSAELPSYVDYGDDIIGFGLNIRGHGNSRDDVNPGFPGYILSNLEDKEKY
                     IYRGAYMDCVRGIDFLCSRPEVDASRIAVEGASQGGALTFATAALANDRIAVCAPQVP
                     FLSDFRDYFIVANWPGNEFIDLVENKKALTWNQVYHTLSYFDIKNLAPMIKAPLIMGA
                     GLMDEVCPPHINFAAYNLVTSEKKYIVYPYAGHGLPDDFSRARMEWIRAKLGLN"
     gene            1704..4133
                     /gene="galB"
                     /locus_tag="PLA17_09730"
     CDS             1704..4133
                     /gene="galB"
                     /locus_tag="PLA17_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HPK85731.1"
                     /translation="MEGRTKTSGLLVAVGIVVLLQGCSQPVPRTHVSFNEGWKFVLAA
                     PDEASRPGFDDSSWRTLDLPHDWSIDGEFSETNPATPGGGALPGGVGWYRKTFRVNAA
                     DKGHMIYISFDGVYRNSEVWINGNYLGRRPYGYSSFRYDLTPWLNYGEVDNVIAVRAD
                     NSAQPNSRWYSGSGIYRNVWLTTTEKIAVDHWGTFVTTPEVSSESALIRVTTQIKNST
                     GSKALIRLETTVYDSSNKRVVRTETDQVEVPVSGTTVTQELTVRSPRLWSVDNPQLYR
                     VVTIIRSGQLLADRYETVTGIRSFEFDREKGFILNGEPVVIRGVCNHHDLGCLGAAVN
                     TRALERQLELLKEMGCNGIRTAHNPPAPELLDLCDRMGFIVMDEAFDIWKVKKTEFDY
                     SLDFDEWHRKDIEAMVLRDRNHPSVFIWSIGNEVMEQWKRDGSGEAIATGLAGIVRSL
                     DDTRPVTAACNDPVPHNPVIASGALDLIGYNYRDTLWTSLPQAFPEGRFIGTETTSAL
                     ATRGSYDMPSEIVRRWPARWDQPFNDGNPDLTCSAYDNCSTPWGSTHRDSWRLIRDNA
                     FLTGMYIWTGFDYLGEPTPYWWPARSSYFGIIDLAGFPKDAYWFYQSEWTDKDVLHIF
                     PHWNWNPGQTVDVWAFTNCDEVELFLNGISLGRQSRNRDDFNLVWKVPFREGTLLGVG
                     YRDGREIMRSEVRTAGEAAALQLSPDRAEIMADGSDLSFITVTVTDKENNPAPHAGNM
                     VRFHIEGPGTIAGVDNGSQTSMEPFKADYRKAFNGRCLVVVKAGKEKGMVRLTASSEG
                     LADATITIKVK"
BASE COUNT         1054 a         1065 c         1261 g          958 t
ORIGIN      
        1 cagcaactgc cggggcacag agtatagtgc ctgctgagtg gaaattcctt acgggtgaca
       61 aacctgagta tattgactcg gcaaagaacg aatactggtg gcatgatatc tccccgctga
      121 ctgtatggga gaagcagggc ttcgcgggtt atgacgggtt cgcctggtac agggtgaagg
      181 tggttgtccc ggtctcgatg aaaaagaatg cacagaagta tgacgggctg atccttaacc
      241 tggggaagat agatgatgct gacgagacct ttttcaatgg ccataaggta ggggcaacag
      301 gcaagatgcc ccctgactat tccggagcct atgatgtgcc acgctcatac ctgattcctc
      361 ctgactacgt aaactgggga ggaaagaaca ccattgctgt ccgtgtttat gacgccggtg
      421 gcgacggagg cctctatggc ggtccggttg agctgaccaa caggggcaat cctgacctgc
      481 tcacccttgg tgccgtggca gcacaggagg atatgatcct gaagggatcg cccgacgtgg
      541 agattctggc cacagttaag agtgagttca ggaagatgta caggggcgct ctgacccttg
      601 agatcgtcac tgacttcggt gatcaggtgt atgacggtta ccgcgaggtg gcagtgaaaa
      661 agatgagcac gggtagtttc ccgttcctgg tagaaaatct gaagccgggg ttctataagg
      721 caacgcttgc actggagagc aagatgggca ataagaagta caacttcaat ttaggttatg
      781 aaccggaaaa gatagtatcg ccggcagacc cccagcctga ttttacggaa ttctggcaaa
      841 gggccaaggc ggaacttgcc actgttgacc cccggtacaa cctgatcagg attgacagtc
      901 tctgcacgga gaagcatgat gtctacctgg ttgagatgcg ttcgctgggc aatgccctca
      961 taaggggctg gtaccgtgtg cctgtaaaac ccggtcgcta tcctgcaata atgcagctgc
     1021 cgggttacag ctcggcggaa cttccttcgt atgtcgacta tggtgatgat ataatcgggt
     1081 tcggtctgaa tatcaggggg cacggcaaca gcagggatga tgtgaaccct ggttttcccg
     1141 gatatattct gagcaacctt gaagacaagg agaagtacat ctacagggga gcctacatgg
     1201 attgcgtcag aggtattgac ttcctctgct cgagacccga ggttgatgct tcacgcattg
     1261 cagttgaagg ggcaagccag ggaggcgccc ttaccttcgc tactgcagcg ctggccaatg
     1321 accggatagc ggtatgtgca ccacaggtgc cgttcctctc tgacttcagg gattatttca
     1381 tagttgcaaa ctggcctggc aatgagttca tcgatctcgt tgaaaacaaa aaggcactga
     1441 catggaatca ggtttaccat acgctcagtt attttgacat aaaaaacctg gccccgatga
     1501 taaaggctcc cctgattatg ggagcaggac tcatggatga agtttgcccg cctcatatca
     1561 attttgcggc ttacaacctc gtcacctctg aaaagaaata tatagtctat ccgtatgccg
     1621 gacacggcct gcctgacgac ttctcccgcg caagaatgga gtggatcagg gcaaagctgg
     1681 gattgaatta aaacattact gttatggagg gaagaacaaa aacgtcagga ttgcttgttg
     1741 cagtcggaat agtcgtcttg ctgcaggggt gttcacagcc tgtgcctcgt acccatgtca
     1801 gtttcaacga aggatggaaa tttgtcctgg ctgcacctga tgaagcatct cggcccggtt
     1861 ttgacgacag cagctggaga acacttgatc tcccgcatga ctggagcatc gatggtgaat
     1921 tcagtgaaac gaatccggcc actcccggag ggggtgctct gccgggagga gtgggctggt
     1981 accgcaagac attcagggta aacgccgcag acaaggggca tatgatttat atctccttcg
     2041 acggtgtcta tcgcaacagc gaggtatgga tcaacggaaa ctatctcggc aggcggccat
     2101 acggctacag ctcgttcagg tatgacctca ccccatggct taattatggt gaggtggata
     2161 acgtgattgc cgtcagggca gacaactccg cacagccaaa ctcgaggtgg tattcaggct
     2221 caggcatcta ccgcaatgtc tggctgacaa ccactgaaaa gattgccgtt gaccactggg
     2281 gtacatttgt caccaccccg gaggtcagca gtgaatctgc cctcatcagg gtgaccacac
     2341 agataaaaaa cagcaccggc tcaaaggctc tgataaggct ggagaccaca gtgtatgact
     2401 cttccaacaa acgcgttgtc agaacggaga cagaccaggt ggaggttccc gtttcaggta
     2461 ccaccgtgac acaggaactt acagtcagga gtcctcgtct atggtcagtt gacaatccgc
     2521 aactgtacag ggtggtaacc atcatacgct caggccagct tcttgctgac agatatgaga
     2581 ctgttacggg tataaggtca tttgagttcg accgcgagaa gggttttatc cttaacggag
     2641 agcctgtggt aatccgcgga gtatgcaatc atcatgacct gggctgtctc ggggcagcgg
     2701 tcaatacccg tgctttggaa agacaacttg agttattgaa ggagatggga tgcaacggca
     2761 tccgcaccgc ccataatccc cctgcaccgg agctgcttga cctgtgtgac aggatgggat
     2821 tcatcgttat ggatgaggcg tttgatatct ggaaagtgaa gaagactgaa ttcgattaca
     2881 gtcttgattt tgatgaatgg cacaggaagg atattgaagc gatggtgctt cgtgaccgta
     2941 accatccgtc ggtctttata tggagcatcg gtaatgaggt gatggagcaa tggaagaggg
     3001 atggcagcgg tgaggccata gccaccgggc tggcaggcat agtcagatcc cttgatgaca
     3061 ccaggccggt gacggcggca tgcaatgatc ctgttcccca taacccggtc atcgcctcag
     3121 gcgcccttga ccttatcggt tacaactacc gcgacacgct gtggaccagc ttgccgcagg
     3181 cattcccgga aggcaggttc attggcacgg agaccacttc ggccctggcc acccgcggaa
     3241 gctatgacat gccgtcagag atcgtcaggc gctggcctgc acggtgggac cagcccttca
     3301 atgatggcaa tcctgacctt acctgctcgg cttatgacaa ctgctcgacg ccctggggca
     3361 gcacccaccg cgacagctgg aggctgataa gggacaatgc ttttctcacc gggatgtaca
     3421 tatggaccgg ctttgactac ctcggtgagc ctactcctta ttggtggcct gcccgaagct
     3481 cttacttcgg tatcattgac cttgccggct tcccgaagga tgcctactgg ttctaccaga
     3541 gcgagtggac cgataaggat gtgctgcata tcttcccgca ctggaactgg aatccgggcc
     3601 agacggttga tgtgtgggca ttcacaaact gcgatgaggt ggagctgttc cttaacggta
     3661 tttcacttgg ccggcagagc aggaaccgtg atgatttcaa cctggtatgg aaggtgccgt
     3721 tcagggaggg cactctcctg ggcgtgggtt acagggacgg acgtgagatt atgagaagtg
     3781 aggtccggac tgccggagaa gctgctgccc tgcagctttc gcccgacagg gcagagataa
     3841 tggctgacgg atcagacctc tcattcatta ctgtaaccgt tactgacaaa gagaataatc
     3901 cggcgcctca tgccggtaac atggtcaggt ttcacatcga aggccccggc accatcgcag
     3961 gtgttgacaa cggaagccag acaagcatgg aaccgttcaa ggcagattac aggaaagcct
     4021 ttaacggaag gtgtcttgtg gtggtgaagg caggaaagga gaagggaatg gtaaggctca
     4081 ccgcctcatc ggaaggactg gcggatgcaa ctataaccat caaggtgaaa taaggagaga
     4141 gtgccggggt ttggctgccg ggcgagggga gtgccgggct cccgctgccg gaccatgtga
     4201 gagggtcgga gttccccact gccgggcgag gggagtgccg gccagatatt ccggatcatg
     4261 ttcccgccag taatcagata aacaccctca tccccacatc gtagctctca cgcagctcac
     4321 gaggggtgca gccttttt
//
LOCUS       DAOPBK010000190         4323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_16738_length_4323_cov_4.157216, whole genome shotgun sequence.
ACCESSION   DAOPBK010000190 DAOPBK010000000
VERSION     DAOPBK010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16738_length_4323_cov_4.157216"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..1504)
                     /locus_tag="PLA17_09735"
     CDS             complement(221..1504)
                     /locus_tag="PLA17_09735"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPK85732.1"
                     /translation="MLLALALIIFYFTFPLLFIWMCRKWSFFRKLGAIVLAYGFGLIL
                     GTAGIFPKGSDGYRTALQGEAVMNTERLEQLIEEGKALPSDIAANKIAGIQDKVYTVS
                     LLVAFPLLLFSLNLKRWLKYAKKGFVSIVLALVAGLVMVTAGFFIWKDAFPDTWKLAG
                     MFEGIYTGGTPNFAALKLILDVDAERFVVLNTYDMIVGAFLVLFFVTVAPSIFRAFLP
                     KFKEDNGVVVDDELVRQETEGLDDFSGMMNRQRIVPLLKALLMSILIAGVGAAVYFLI
                     PAHLQQYRMAGMVLTITTLGIIASLYRPINKIEKTFQLGMYLILVFSLTVASMSDLRT
                     MFTKGMLDLILFITWCYFGSLVLHIILAKIFRIDADNFLITATAFIFSPPFVPLVANA
                     LKNKDVIVTGIAGGVIGYTLGNYFGTTLAFFLQRF"
     gene            complement(1804..3462)
                     /gene="recN"
                     /locus_tag="PLA17_09740"
     CDS             complement(1804..3462)
                     /gene="recN"
                     /locus_tag="PLA17_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPK85733.1"
                     /translation="MLRRLTVRNYAIIKDLNMEFGEGFTIITGETGAGKSILLGALGL
                     VLGERADSSALLSQDEKCIVEACFNIGGYDLNELFEANEIDYDEEAIFRREITPAGKS
                     RTFLNDTPVNLAVLKEIGSRLVDVHSQHETLLLGAGSFQMRVVDAYAGISNLSGDYSR
                     AWKGYLSARKDYDTAMAGLEKAKADFEYYSHQLEEFRAVKPVAGEQELLEKEQEMLSN
                     ASEIKEALAAVTEALGGEEISALSLLRTVRTNLARISGWLPGAAELEKRVDVQLIDLN
                     DISYEADKLNDRATADPGRLEYVTQRLDSLYSLMHKHRCRDIDELLAVQKRIEADVAA
                     SSDTDEKAEELRKRRDAAMAQVNSLAGKISEGRRKAVAPLSREITGMLHRLGMPHARF
                     EASVTPLPAPGPSGIDDIRFLFSANRQVEPEELSKCASGGELSRVMLCLKSVLANSSG
                     LPAIIFDEIDSGVSGEVASMVGSILAEMGRSMQVINITHLPQVAARGRVHYHVYKEDG
                     DHSTITRVRLLNDDERVMEVARLLSGSTVTDAAMRNARELMKDN"
     gene            complement(3473..>4323)
                     /locus_tag="PLA17_09745"
     CDS             complement(3473..>4323)
                     /locus_tag="PLA17_09745"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPK85734.1"
                     /translation="YCNVQVSAQKIQGSNREVFQNMQRDIYEFMNSMVWTDNIFSFSE
                     RIECNLLINLDEQLSADEFRGTIQIQLSRPVYNTTYESTVLNFVDNNFQFRYVEFQPL
                     EFNPNSHTSNLVSVLAYYAYLLIGMDFDTYSQGGGTPYFQVAEKIVINAQNAPEQGWK
                     PYDGSRNRNRYWLVKNILDSEYAGVRQFIYLYHLRGLDRMESDMTRARTEIYESLRRL
                     QEVYRQRPDPFMYYMTVVLDAKADEIVNIFSQAFPEEKNRVVQVLTEIDPANETKYKK
                     ILTSNS"
BASE COUNT         1105 a         1148 c          962 g         1108 t
ORIGIN      
        1 aagtgtaatt ttaagatgta aaacttcgat gaaaacctgc caccccagac acactaattg
       61 gaacactacc ttctatatag gacttaacta cctgttttag gagtccgaag accccgaagc
      121 cgaagactga aggacttaag acctgagggt cagggagatg taaactgggt ttatccacgt
      181 tccccggacc ctggattttc aggctttacg gccctgcctg tcagaaccgc tgcaggaaaa
      241 atgcaagtgt ggttccaaag tagttgccaa gagtgtatcc gatcactccg ccggctatgc
      301 cggtgacaat cacatccttg tttttcagcg cattggccac cagcggaaca aacggagggg
      361 agaagatgaa agccgtggcc gtgatgagaa aattgtcggc atctatcctg aatatcttgg
      421 ccaggattat gtgaagaacc aaagatccga agtaacacca tgttataaac aatatcagat
      481 ccagcatacc ctttgtgaac atggtcctca gatcagacat ggaggctacc gtaagagaga
      541 agacaagtat gaggtacatt cccagctgaa atgtcttctc tatcttattt attggtctgt
      601 agagtgaagc aataatgcca agcgttgtga ttgtaagcac cattccggcc attctgtact
      661 gttgcagatg agccggtatg agaaaataga cagcagcgcc aacccccgct atcagaatgc
      721 tcatcagcaa tgctttcagg agcggaacta tccgctgtct gttcatcatt cctgaaaaat
      781 catccagacc ctcagtctcc tgcctgacca gttcatcatc cacaacaacc ccgttgtctt
      841 ccttgaattt tggaaggaaa gccctgaaaa ttgaaggtgc cacagtcacg aagaacagta
      901 ccaggaatgc acccacaatc atatcataag tgttcagtac aacaaaacgt tcagcatcaa
      961 catccaggat cagcttcagg gctgcaaagt tcggggtacc gccggtatat atgccttcaa
     1021 acatgcctgc gagtttccag gtgtcaggga aggcatcttt ccatataaag aaaccagcag
     1081 ttaccattac aagccccgct accagggcga gaactattga tacgaaaccc ttctttgcat
     1141 atttgagcca tcttttcaga ttaagtgaaa agagaagtaa agggaaagcc acaagcaacg
     1201 aaacagtata aaccttatcc tggatacccg caatcttgtt tgctgcaatg tctgaaggga
     1261 gagccttccc ttcttcaatc agctgttcaa gacgttcagt attcattaca gcctctccct
     1321 ggagcgctgt ccggtaccca tcacttcctt tcgggaagat tcctgcagta ccgagaataa
     1381 gtccaaaacc gtaggcaaga actatcgctc ccagttttcg aaaaaaactc catttcctgc
     1441 acatccagat aaatagcagg ggaaatgtga aatagaatat gatcagggca agagctaaaa
     1501 gcatggcggt taattttagg taaccaaatt taaacaaagc acactgccct taggtcaggg
     1561 tgccggcaaa agaggtatta atacttctgc aatataggta tttctactga tcggaacact
     1621 cccggaaact attgttgcac tgttcaataa cccgcacact cattcccatg cctgagactg
     1681 ccatcattga gtatgttctg tattgtgatt ttttttgact ccgggcaggt ttgtgaccat
     1741 gattatgctg tttcggcttt actcagccat cagacgccag ctataagaca tcagccgcct
     1801 gactcagttg tccttcatca gttcacgggc gttgcgcatt gcagcgtcag tgacagtgct
     1861 gccgctgagg agcctggcca cctccattac cctttcatcg tcatttaaca gtcttaccct
     1921 cgtaattgtc gagtggtcgc catcctcctt atatacatgg tagtgtaccc ttcccctggc
     1981 agccacctgc ggaagatgcg tgatgtttat cacctgcatg ctcctgccca tctctgccag
     2041 aatagaacca accattgagg ccacctcacc ggatacacct gaatcgatct cgtcaaagat
     2101 tattgccggc agtccggagc tgtttgccag caccgatttc aggcagagca tcacccttga
     2161 cagctcgccg ccggaggcgc actttgacag ctcctcaggt tcaacctgcc tgtttgcgct
     2221 gaagaggaac ctgatgtcat caattcccga gggccccggc gccggcaaag gcgttacgga
     2281 agcttcaaac ctcgcatggg gcattcccag cctgtgaagc attccggtta tctcgcgtga
     2341 gaggggtgcc actgccttgc gcctcccttc tgatattttt ccggcaagtg aattcacctg
     2401 agccatggca gcatcccgcc tcttcctgag ctcttcagcc ttctcgtcag tatcggatga
     2461 ggcggcaaca tctgcctcaa tcctcttctg aacagccagc aactcatcaa tatccctgca
     2521 acggtgctta tgcataagtg aatagaggct gtcaagcctc tgagtcacat attcaagcct
     2581 gcccggatcg gcagtcgccc tgtcgttgag cttgtcagcc tcatatgaga tgtcattcag
     2641 gtctatcagc tgtacatcca ccctcttttc cagctctgct gccccgggaa gccatccgga
     2701 gatacgtgca aggtttgtcc ttactgtccg gagcagtgaa agggcagata tttcttctcc
     2761 tccgagagct tcggttactg cagccagtgc ctccttgatc tccgaggcgt ttgagagcat
     2821 ctcctgttcc ttttcaagca attcctgctc gccggcaaca ggtttcacag cccggaactc
     2881 ttccaactgg tgtgaatagt attcaaaatc agccttcgcc ttctcaaggc ctgccatagc
     2941 ggtatcataa tccttcctgg ctgaaagata ccctttccat gcccgggaat aatctcctga
     3001 aaggtttgat atccccgcat aggcatcaac cacacgcatc tggaaggaac cggctccgag
     3061 gagcagggtt tcatgctgcg agtgaacatc aacaagcctg ctgccaatct cctttagaac
     3121 tgcaagattg acaggcgtgt cattgaggaa ggtccttgat tttccggcag gtgttatttc
     3181 acgcctgaat atagcctcct catcataatc aatctcattt gcctcaaaca gttcattgag
     3241 gtcatatccg ccaatgttga aacatgcctc aacgatacac ttctcatcct gactgagcag
     3301 cgccgaagaa tcagccctct ctccaaggac aagaccaagg gcgcccagaa gaattgattt
     3361 tccggctccg gtttcacccg ttataatggt aaaaccttca ccgaactcca tgttcagatc
     3421 cttaatgatg gcatagttcc ttaccgtgag tctgcgcagc atcaggaggg aattaactgt
     3481 tggaagtcag tatcttttta tatttggtct cgtttgcagg gtcaatctcg gtcagcacct
     3541 gcaccaccct gttcttttcc tcggggaaag cctgtgaaaa aatattgaca atctcgtctg
     3601 ccttcgcatc aagcaccacg gtcatatagt acatgaacgg gtcaggccgt tgcctgtata
     3661 cttcctgtag ccttctgaga ctctcataaa tctctgtccg cgcccgggtc atatctgact
     3721 ccatccggtc aagtcctctc aggtgataga gataaataaa ctgcctgaca cccgcatact
     3781 cactgtcgag aatattcttt accagccagt accggtttct gtttctggaa ccgtcatatg
     3841 gtttccagcc ctgttcaggg gcattctggg cattgatgac tatcttttcg gcaacctgga
     3901 aataaggggt tccgccaccc tgtgaatagg tgtcaaaatc catcccgatc agaagatagg
     3961 cgtagtaggc cagcaccgat accaggttgc tcgtgtggct gttcggattg aactccaggg
     4021 gctgaaactc cacatacctg aattgaaaat tgttatccac gaagttgagc actgtagact
     4081 cataggtggt gttgtaaacc ggcctgctga gctgtatctg tatagtgccg cggaactcat
     4141 cagccgaaag ctgctcatca aggttgatca gcaggttgca ttcgatcctt tctgaaaagc
     4201 tgaagatatt gtcagtccag accatgctgt tcatgaactc gtaaatgtca cgctgcatgt
     4261 tctgaaatac ctcacggttc gaaccctgga ttttctgagc cgacacctgc acgttgcagt
     4321 aca
//
LOCUS       DAOPBK010000191         4280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_16953_length_4280_cov_3.032663, whole genome shotgun sequence.
ACCESSION   DAOPBK010000191 DAOPBK010000000
VERSION     DAOPBK010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16953_length_4280_cov_3.032663"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLA17_09750"
     CDS             <1..519
                     /locus_tag="PLA17_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPK85735.1"
                     /translation="GGPLFFTHYSFLGLDPRNFQDTWVNYLEQNTAHSLINWKHCETN
                     PFSYVGYSGECWGLTASDNHKGYSAHSPSNDLGVITPTAAISSMPYTPEQSMNAIRHF
                     YYILGDRLWGEYGFHDAFNPTEGWWATSYLAIDQGPIICMIENHRTALLWDLFMSAPE
                     VQAGLDKLGFTY"
     gene            526..1905
                     /locus_tag="PLA17_09755"
     CDS             526..1905
                     /locus_tag="PLA17_09755"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPK85736.1"
                     /translation="MKKIYFILLAAIVSAACNQPQKTVFRSQGKVDYLLSSEDEAMLD
                     SIQKKTFLFFMGEHHPEWGIVRDRTASWAPASIASTGFGIPSFAIGVERGWITRDEAA
                     QITLNMMNFFVNSLQSPDTLASGYKGFYYHFLRMDTGLREWRCELSSVDTGLLMMGIL
                     FARNYYDRDNETENQIRSQGSLMLGNIEWDFMMMPDSGNYARQISMGWHPESGLHKMG
                     WSGYNEALFLYILAAGTGLENAKESYRSWLSSYKWQTPYEGLSHVAFPPLFGHQFSHC
                     FIDFRGLADEYMRDKGIDYFENSRRATRVQRQYAIDNPHGWVGYDSLCWGVTASDGPG
                     DKYNFGEREFLGYAGRGTSGPEYNYFDDGTIAPYGPLSSLPFAPEIVIPTIRSINEKM
                     GDKIWGRYGYYDSFNLTAGWVNDDFIGIDQGPMLIMIENFRTGLVWNYVMNDPVIQKG
                     LQTLGFSYL"
     gene            2085..>4280
                     /locus_tag="PLA17_09760"
     CDS             2085..>4280
                     /locus_tag="PLA17_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPK85737.1"
                     /translation="MNYRTGKTELLLLLLIGLTSFAATAQVRILESFEQASGWSYNIS
                     DGVTLNISAEKGVTGNAVRIDYDFTEGTGYGGIQKLFPIDLPDNYEFTFWLRAESPSN
                     NFEIKFIDSTGNNVWWVNNRNYDFPREWTRIRVKKRHITFAWGPTEDRDMKRVDRIEF
                     TVASFVGGKGTLWLDDLKFEPLPPETDLWPEPAVSASSSSRGHSPELAADGSAESCWQ
                     SRRAGRQSITLDFKGRREFGGLDIAWLEGSHAEEFDILLSDDGNKWGKAYSVSPNRSS
                     SSIIRLPEAEAAYLRIDLLKPANGRYYGIREIKIPDVKSTLTPNDFIIYAARNSPAGS
                     YPGYFSGRASYWTVTGVSSDTKEALISEDGMVEAEKARFSLEPMIKTANRLINHSNVR
                     PEQGMGYPGYTGDYACLPSVEWKQDGIRFVTGVSSGGRPNDDSELYIGYWFENISAEP
                     QELEFYLLLRPFQVNPYYQFLNTVGGVGRIMSVREISKGLISVDGKPVRLTEAYESFG
                     ASKFDEGNQVEMIRRGEMPVSPGVTDPSGMAGAIVKYRIKLEPGESKELFAVIPYHSV
                     IGESSGPEAGENPPQVIRAESPAEGGALHMLPQSTAEAREKFFASADYWNKRTGHIRF
                     DLPPSADRLIDTWRSNLAYILINRDMAGIQPGSRSYDRSWIRDGSLTSSALLKSGIVA
                     EVKEFIEWYAANQYDNGKVPCVVDFRGPDPVPENDSHGQLIYLIREYFNF"
BASE COUNT         1103 a         1035 c         1165 g          977 t
ORIGIN      
        1 ggcggcccgc tcttcttcac gcactattcg ttcctggggc tcgatccgcg aaactttcag
       61 gatacctggg ttaattacct cgaacagaac acggctcatt cacttattaa ctggaagcac
      121 tgcgagacaa acccgtttag ttacgtaggg tacagcggtg aatgctgggg cctgacagcc
      181 agtgacaacc acaagggtta cagtgctcat tcaccgtcga atgatctggg agtaatcact
      241 cccacagcag ccatatcatc aatgccgtac actccggaac agtcaatgaa tgcaatcagg
      301 catttctatt atattctcgg agaccggcta tggggtgagt acggtttcca cgatgccttt
      361 aatccgactg aaggatggtg ggcaacatca tacctggcca ttgaccaggg tccgataatc
      421 tgcatgattg agaatcatcg tacagctctt ctctgggatc tgttcatgtc agctccggaa
      481 gtgcaggccg gccttgataa actgggattt acatattgaa agcctatgaa aaagatatac
      541 ttcattttac tggcggcaat tgtatcagcc gcatgcaatc agcctcagaa gacagttttc
      601 cgctctcagg gaaaggttga ctatctgctg tcatccgaag atgaggcgat gcttgactcg
      661 attcagaaaa agactttcct ctttttcatg ggagaacatc acccggagtg gggaatagtc
      721 agggaccgca ccgcatcatg ggcgccggca agcatagcct ccaccgggtt cggcatcccc
      781 tcgttcgcca ttggcgtgga acggggctgg ataacacggg atgaggcggc acaaataacc
      841 ctcaacatga tgaatttttt cgtgaattca cttcagagtc ctgatactct ggcttccggg
      901 tacaagggat tttactatca ttttctccgg atggatacag gcctcaggga atggagatgc
      961 gagctctcct cggtagatac cgggctgctt atgatgggga tactctttgc aaggaattac
     1021 tatgaccgtg acaacgaaac ggaaaatcag atccgctcac aggggtcact gatgctcggg
     1081 aatattgagt gggacttcat gatgatgcct gattcaggta attatgccag gcagatatcc
     1141 atgggctggc accctgagtc agggcttcat aagatgggat ggagcggtta caatgaggcg
     1201 ctgttcctct atatccttgc cgcaggaaca gggcttgaga atgcaaagga gagctacagg
     1261 tcatggctca gcagctacaa gtggcaaacc ccgtatgaag ggctttcaca tgtggctttt
     1321 cctccgcttt tcggccacca gttctctcac tgcttcatcg acttcagggg gctggccgat
     1381 gagtatatgc gcgacaaggg gatagactat tttgagaact cgcgccgggc aacccgggtt
     1441 cagcggcagt atgccatcga caatcctcac ggatgggtcg ggtatgactc actatgctgg
     1501 ggcgttacag caagtgacgg cccaggagat aaatacaact tcggcgaaag ggagtttctg
     1561 ggctatgccg gaagaggaac cagcggacca gagtacaact actttgatga tggtacaata
     1621 gcaccgtacg ggcccctctc ctcactgcca tttgcaccgg agatagttat cccgacaatc
     1681 aggtcaataa atgagaagat gggcgacaaa atctggggca ggtacgggta ttatgattca
     1741 ttcaacctca cagcaggatg ggtcaatgat gatttcatag gaattgatca gggccctatg
     1801 ctcataatga tagagaactt cagaaccgga ctggtctgga actacgtgat gaatgatccg
     1861 gtgattcaga aaggccttca gacgctcggg ttttcatacc tgtagcgctt taaccctgac
     1921 cctgcctgaa acagtcggca gcaatcgtca gaagaaactg ccggcagcaa tcgccggtaa
     1981 aaactataaa caaaagccgt caacagaaac cctcagaaga aatcaccagc agaaaccctc
     2041 agaagaaact gccggcagca atcgccagaa gaaacaccca tatcatgaat taccgaaccg
     2101 gaaaaacgga actcctcctg ttgctgctca ttggcctgac ttcatttgcc gcaacagccc
     2161 aggtacgaat acttgagtcc tttgagcagg cttcaggctg gtcatataac atctctgacg
     2221 gtgtaaccct gaacatatct gctgaaaaag gggtaaccgg taacgccgtc aggattgact
     2281 atgatttcac cgagggaacc ggttatggcg gcatacagaa gctgttcccg atagacctgc
     2341 ctgataatta cgaatttacc ttctggctaa gggctgaatc accttccaac aatttcgaaa
     2401 taaaattcat tgacagcacc gggaacaatg tctggtgggt caataaccgc aactatgatt
     2461 ttccacgtga gtggaccagg atccgcgtaa agaaaaggca tatcactttt gcctggggtc
     2521 cgacagagga ccgcgatatg aagcgggtag acaggattga attcaccgtt gcctcctttg
     2581 tcggagggaa gggaactcta tggcttgatg atctgaagtt tgagcctctg cctccggaga
     2641 cggatctctg gcctgagccg gcggtgagcg cctcctcctc atccaggggt cacagtccgg
     2701 agcttgcagc tgacggatca gctgaatcct gctggcaaag caggagggca ggcaggcaga
     2761 gcataaccct tgatttcaag gggaggaggg agttcggggg acttgatatc gcatggcttg
     2821 aaggcagcca tgccgaagag tttgacatct tgctttcaga tgacggaaac aaatggggaa
     2881 aggcatattc ggtcagcccc aaccgcagca gttccagcat catcagacta ccggaggccg
     2941 aggcggctta cctccgcatt gaccttctga aaccggctaa cggcaggtac tatggcatca
     3001 gggagataaa gattcctgac gtaaagagta cccttactcc caatgatttc ataatatatg
     3061 ctgcaagaaa ctcgcccgca ggaagttatc ctggttattt cagcgggcgg gcctcatact
     3121 ggacagtcac cggggtcagc agtgatacta aggaagcgct gataagcgag gatggtatgg
     3181 tggaagctga aaaagcgcgc ttttcactgg agcccatgat taaaaccgct aacagactca
     3241 ttaaccatag taatgtccgg ccggagcagg ggatgggcta tcccggatat accggtgatt
     3301 atgcctgtct tccttccgtt gagtggaagc aggatggaat caggtttgtg accggtgtct
     3361 cttcgggcgg caggcccaat gacgattcgg agctttacat cggttactgg tttgaaaaca
     3421 tttcggcaga gccacaggag ttagagtttt accttctgct tcgcccgttt caggtgaatc
     3481 cgtactatca gtttctcaac accgttggcg gcgtaggaag gataatgtca gtcagggaga
     3541 tcagcaaagg tctgatctcg gtagatggca agccggtcag gctgactgag gcgtatgagt
     3601 catttggcgc ctcaaagttc gatgagggca atcaggttga gatgattcgc aggggggaga
     3661 tgcctgtttc gccgggtgtg accgatccct cggggatggc aggcgcgatt gtgaagtaca
     3721 gaataaagct tgaaccgggt gagtcgaagg aattatttgc cgtaatcccg taccacagcg
     3781 ttatcggaga atcctccggg ccggaggcag gtgagaatcc gcctcaggta atcagggcag
     3841 aatcaccggc tgagggcggc gcactgcaca tgcttccaca gtccactgct gaagccaggg
     3901 agaagttttt cgcgtcagcg gactactgga acaaaagaac aggacacatc cgttttgatc
     3961 ttcctccttc ggctgacagg ctgattgaca catggcgctc aaacctggcg tatatactga
     4021 tcaacaggga catggcaggc atacagccgg gctcgcgttc gtatgacagg agctggatcc
     4081 gtgacggatc gcttacatca tcagcccttc tcaaatcagg catcgtcgca gaggtgaagg
     4141 agttcattga atggtatgcg gcgaaccagt atgataacgg aaaggtgcca tgcgtggtcg
     4201 acttcagggg gcccgatccc gtgcctgaaa atgacagcca cggccagctg atttatctta
     4261 tcagggagta tttcaatttc
//
LOCUS       DAOPBK010000192         4258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_17047_length_4258_cov_4.266476, whole genome shotgun sequence.
ACCESSION   DAOPBK010000192 DAOPBK010000000
VERSION     DAOPBK010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17047_length_4258_cov_4.266476"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLA17_09765"
     CDS             <1..468
                     /locus_tag="PLA17_09765"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK85738.1"
                     /translation="VLTTFSYRYNNFIIARYADILLLYAEACAQTNDPDGLQYLRQVQ
                     ERAGSAHISSSLTLDEVKKERNYELWCEGARWIDMKRWGEFDKAKNAGKHIPSLKDAF
                     FVDGEATHRGYVTYSEPNADKQCGFVAGKHEWFPYPYSVTSINPSLQQNPGWD"
     gene            complement(758..1837)
                     /locus_tag="PLA17_09770"
     CDS             complement(758..1837)
                     /locus_tag="PLA17_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPK85739.1"
                     /translation="MSIELRKEYACSLLVPTSMGVRITPLNHQPVHSSDTFFMHVTSA
                     ETNVASIASYLGKPVKVLTTFVRDSPIAQLIKNNLSGRRMTYEGREVEQGGPWGYRHQ
                     FNVADMGYGSRGPRVHNDRAGEVGRTLNVKDFDLERIFGKEGVQIVHLSGLIGALSHE
                     TSIFCLEIAREAKKHGTLISFDLNYRASFWEGREKELSGVFREIASVADILVGNEEDF
                     QLCLGIEGPETGGKDIGARMESFRQMIDRVRKEYPGASVFANTLREVKSADSHLWGAI
                     MWEGGNWHIIEPREIRVLDRIGGGDGFVGGLLYGILKGWEPARWIEFGWACGALATTF
                     LTDYAQPSDEEMVWSIFEGDARVRR"
     gene            2033..2689
                     /locus_tag="PLA17_09775"
     CDS             2033..2689
                     /locus_tag="PLA17_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPK85740.1"
                     /translation="MKKRIIGFAVILIMYSLNTPVMIYSQDISLPSPSRKGGMPLMEA
                     LDARVSARLFSDKDLSLQQLSDLLWAAWGINREDGKRTAPSSHNRQEMDVYVTLRSGL
                     YLYDAAACRLKQIHNRDIRALTGTQEFPATAPVNLVFVADLAKRGLKEGQQVTDTDKL
                     SSWSNTGFMAQNVYLWCASEGLSCVIRAMVPRDKLAPEMELKPLQVIILAQSVGFPGE
                     "
     gene            complement(2713..4056)
                     /locus_tag="PLA17_09780"
     CDS             complement(2713..4056)
                     /locus_tag="PLA17_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPK85741.1"
                     /translation="METPGDNGIDFRHPELWDEPDYIRKEIACTMSHIYRYGLTTTSG
                     GNISVTDSLGNLWITPSGTDKGSLKPGEVVCVTHDGTWEGPFRPSSEYPLHRSVYAVR
                     PDIRALIHAHPPLLTSFSIAHRVPDTSVVGAWRETCGAVGYAGYAIPGSEAMGETVAR
                     EFMKGHNTVIMENHAVVVGGRDLAEAQARLETLEKCARTIHAAGFPGKPVITENASGA
                     NPEFNSGNYLPETGRMLPGVTGNEMSPDEELLAEEICRTAARACRMGLIYGFCGTLSA
                     RSAGDNWLVTREKVLRCNIGKGDINVYGSPGNHTIPGERLHAEIYRRFPAVRAVIVAR
                     PPCLMAFAVTGKEVSVRTIPESWILLQEMPLVPFGALSPGREEIFEKLSAGIPVVLIA
                     NDSVLVTGDSLLRAFDRLEVAEMTAMSLILGESLGSVKPISDNNIAELGRVFFSK"
BASE COUNT         1012 a         1186 c         1015 g         1045 t
ORIGIN      
        1 gtgctgacaa ctttctcata ccggtataac aacttcatca ttgccagata tgctgacatc
       61 ctccttctgt atgccgaggc ctgtgcccag actaatgatc ctgatggcct tcagtatctc
      121 cggcaggttc aggagcgtgc tggatcagca catatcagct cttcattgac tctggatgaa
      181 gtgaagaaag aaagaaacta tgaattgtgg tgtgaaggcg cacgatggat tgatatgaaa
      241 cgctggggtg aatttgacaa ggcaaagaat gcaggaaagc atattccttc actcaaagat
      301 gccttctttg tcgacggaga ggctacccac agaggctatg tcacatactc agagcccaat
      361 gctgacaagc agtgtggctt cgtggccggc aaacacgaat ggttccccta tccatacagt
      421 gtgacttcaa ttaaccccag cctgcagcag aatccgggat gggattagac tacagaaact
      481 ttaaaactgg aagaagggtt cagattatgt taaattcaaa accggccgga agggatttca
      541 ttcacttata cttgccgtcc tgctggctta agttgacaga aatccgactt ttttaatcag
      601 taagtgaggt ttggttcgaa agagggtgtc tcaaaagggc accctctttc ttattccatg
      661 gcggtagtct gtatgggcac caggctcctt cttccacaaa caggagagca gggaagggag
      721 attaaaccct gcctttgtta tttatttgaa tcatcattca gcgtctgact ctcgcgtcac
      781 cctcaaagat gctccacacc atctcctcgt cagagggctg ggcataatcg gtaaggaagg
      841 ttgtagccag tgcaccgcag gcccatccga actcaatcca tcttgccggc tcccatccct
      901 tgaggatgcc gtacagcagt ccgcccacga agccgtcgcc gccgccaatg cggtcaagga
      961 cgcgtatctc acgtggctca atgatatgcc agtttcctcc ctcccacatt atggcacccc
     1021 agaggtggga atcagcactc ttcacctcgc gcagggtgtt ggcaaaaacc gatgctcccg
     1081 gatactcctt tctgaccctg tcaatcattt gcctgaagga ttccatcctg gcgccaatat
     1141 ccttccctcc tgtttcggga ccctcaattc ccaggcaaag ctggaaatcc tcctcgttgc
     1201 ccacgaggat gtcagccacg gaagctatct cacggaagac tcctgagagc tccttctccc
     1261 gaccctccca gaatgaagcg cggtaattga ggtcaaagga gatgagtgtg ccgtgcttct
     1321 tcgcttcgcg tgcaatctca aggcaaaaga tgctggtttc atgtgacagc gcccctatca
     1381 ggcctgagag gtgaacaatc tgcaccccct cctttccgaa gatgcgctcc aggtcaaaat
     1441 ccttcacatt cagtgtgcgc cccacctcgc cggcacggtc attatgcacc cggggaccac
     1501 gcgaaccgta acccatgtct gcaacattga actggtgacg gtatccccag ggtccgccct
     1561 gctctacctc cctgccctca taggtcatac gccggcctga gaggttgttt ttaatgagct
     1621 gagcaatggg actgtccctg acaaatgttg tcagtacttt aaccggcttc cccaggtaac
     1681 tggctatgct tgcaacgttc gtctcggcgc tggtcacatg cataaagaag gtgtcgcttg
     1741 aatggaccgg ctggtggttt aacggggtga tgcgtacacc catgctcgtg ggaacaagca
     1801 atgagcaggc atattctttt cttaattcta tactcataga ctcttcggtt tttcagattc
     1861 ctgtttcaca atgccgtata taaatcctac tgccggccgg ataaaattat cactttttcc
     1921 tgaccggaag gtgaagtttc cggtcaattg tcagcattga cctgacagct gaccgccgga
     1981 aaagtgaatt ttcctgacat ttttgttaac ttcaccgcaa atcagggtaa agatgaaaaa
     2041 aagaatcatt ggttttgctg tcattctgat aatgtactca ttaaacactc cggtcatgat
     2101 atactcacag gatatttctc tgccttctcc atcacggaaa gggggcatgc cgctgatgga
     2161 agcccttgac gccagggtat ctgcaaggct tttttcggac aaggatctgt cactgcagca
     2221 gctctccgac ctcctctggg cggcctgggg aatcaaccgc gaggatggca aacgtactgc
     2281 accctcctcg cataacaggc aggagatgga tgtctatgtg actctcagga gcggcctgta
     2341 cctctatgat gccgctgcct gcaggctgaa gcagatacac aacagggata tacgtgccct
     2401 gacaggcact caggaattcc ccgcaacagc ccctgtcaac ctggtgtttg ttgctgatct
     2461 tgccaaaaga ggccttaaag agggacagca ggtcacagac accgacaagt tgtcgtcatg
     2521 gtccaacacc ggttttatgg cacagaatgt ctacctgtgg tgtgcctctg aagggttgtc
     2581 atgcgtcata agggcaatgg ttccgcgtga caaactggca ccagagatgg aactgaagcc
     2641 gctgcaggta ataattcttg cacagagtgt cggcttccca ggggagtaac tgtcccttca
     2701 gttatttgca tgtcacttgc tgaaaaagac ccttcccagc tccgcgatat tgttatctga
     2761 aattggtttg acactcccta gtgactctcc gagtataagt gacatggcag tcatctcggc
     2821 cacctccagc cggtcaaatg cccggagcag gctgtcaccg gttaccaata ccgagtcatt
     2881 ggctatcagc acaacaggga tacccgcact tagcttttca aatatctcct cccggccagg
     2941 tgaaagtgcc ccgaagggta cgagaggcat ctcctgcagt aaaatccagc tctccgggat
     3001 ggttctgaca ctgacctctt tacctgtaac cgcgaaagcc atcaggcagg gaggccgggc
     3061 aacaatcacc gccctgacag caggaaacct ccgatagatc tcagcatgca gcctttcacc
     3121 gggaatggtg tggtttcccg ggcttccgta aacattaatg tcacctttcc cgatattgca
     3181 tcttaaaacc ttctcacggg taacgagcca attgtctcct gcgctccttg cagaaagagt
     3241 cccgcagaaa ccgtaaatca gtcccatcct gcatgcccgt gctgccgtac ggcaaatttc
     3301 ctccgccagc agttcttcat caggactcat ctcattgcct gttacccccg gcagcatcct
     3361 gccagtttcc ggaagataat ttccggagtt gaattcagga ttggctccac tggcattttc
     3421 agtgatcaca ggcttccccg gaaatccggc agcatggatc gtcctggcgc atttttccag
     3481 agtctcgagt cgcgcctgcg cttctgccag gtccctgccg ccgacaacca cagcatggtt
     3541 ttccataata acggtattgt gacccttcat gaactcccgg gccactgttt ctcccatagc
     3601 ctcgctgccc ggaattgcat acccggcata cccaacggct ccacaggtct ccctccacgc
     3661 tcccaccacc gatgtgtcag gaacccggtg cgcaatgctg aaagaggtca gtaacggcgg
     3721 gtgggcatgt atcagagccc tgatgtcagg tctcacagca taaacgctac ggtgcagggg
     3781 atattccgag gatggtctga acggaccttc ccaggtcccg tcatgggtga cacacaccac
     3841 ctcaccgggc ttcagtgatc ccttgtcagt gcctgacggg gtgatccaga ggtttccaag
     3901 gctgtcagtg acagatatat ttccccctga ggtggtagtc agcccatacc tgtaaatatg
     3961 gctcattgta caggctatct ccttccggat ataatccggc tcatcccata gttcaggatg
     4021 tctgaaatca atgccattat ccccgggtgt ctccatgctg caggttattt ttaatccgga
     4081 gaaattattc tccggaacaa ggtataaaga tagcaagaag attgctcggc atgtcgtgga
     4141 cattctctga atatgatctt ccctatgcct tcctgctcag caaagactta ttcctgcatc
     4201 atcttcaggt agtccacaat cccctcaaga atcttgaccg ccatcagctg gcggaact
//
LOCUS       DAOPBK010000193         4203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_17337_length_4203_cov_6.320636, whole genome shotgun sequence.
ACCESSION   DAOPBK010000193 DAOPBK010000000
VERSION     DAOPBK010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17337_length_4203_cov_6.320636"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLA17_09785"
     CDS             <1..450
                     /locus_tag="PLA17_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK85742.1"
                     /translation="DLFFEFMITEGANSGVKYFVTEAYPTEGSAIGLEYQILDDQRHP
                     DALLGRDGNRTVASLYDLIPARNKRFNGVGQWNAAQIVSLNGHVEHWLNGFKVLEYER
                     GSDEYRRLVSESKYKIFEGFGEAEQGHILLQDHGDEVWFRSIRIREL"
     gene            complement(787..1914)
                     /locus_tag="PLA17_09790"
     CDS             complement(787..1914)
                     /locus_tag="PLA17_09790"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPK85743.1"
                     /translation="MRKLNLFLGSVLVLFMFGISSCGNKAKEMVTTENFGSFDGKEVS
                     LFTLTNAKGDVVKLTNYGAAIVEVNVPDRNGDKANITFGYETLDGYLAGDAYFGKVVG
                     QYANRIAKGKFTLDEVEYTLALNNFPNSLHGGPAGWHSRVWTAEVLKGTDFPAVKFTY
                     NKPDMEEGYPGNVVAEVVYNWTDDNELIMDYKVTTDRRSVINITNHAYFNLHGSGNGD
                     ILDHEVLIRASAFTPVDPTLIPTGELRPVEGTPFDFRTPHLIGERIGEEYDQLILGRG
                     YDHNFVLDNVEEVDAEVYDPSTGRVLQVITDQPGVQLYTGNFLDGTQTGRGGKVYQYR
                     SGLCLETQHFPDSPNQASFPSVILTPEEPFLSSTTYKFGVR"
     gene            complement(2093..3622)
                     /locus_tag="PLA17_09795"
     CDS             complement(2093..3622)
                     /locus_tag="PLA17_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HPK85744.1"
                     /translation="MIMSALTFSAFGQPGEKTGKANIIISTDLGAQTISRHIYGHFSE
                     HLGHCIYGGYWVGEGSSIVNTRGIRNDVVEALKRTQIPNLRWPGGCFADEYHWMDGIG
                     PREKRPKMINTHWGGVTEDNSFGTHEFLDLCEQLGAEPVICGNVGSGSVEEMSKWVEY
                     INFDGVSPMADLRRANGRDKPWNVKYWGVGNENWGCGGNMTADFYADQYRRYATFARN
                     YGDNRLMKIAGGANSTDYNWTEVLMRDVPHNLMWGISLHYYTTNWENKGSATKFGEKE
                     YFETLERCLNVEKALDRHITIMNKYDPAKRIALVVDEWGTWWDVEPGTNPGFLFQQNT
                     LRDAVVAGLSLNYFNERCDRIRMANIAQTVNVLQSLILTDGEKMLLTPTYHVFEMYKV
                     HHDATLLPLDVNCGTYAMDGRELASISASASKDADGTVHISVVNIEPSEKINLLIDLR
                     GGDFKEVSARILTAPELNTHNTFEKPEEVKPAEFKDVKLKKNILNLDMPAKSIIVFEV
                     K"
     gene            complement(3828..>4203)
                     /locus_tag="PLA17_09800"
     CDS             complement(3828..>4203)
                     /locus_tag="PLA17_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85745.1"
                     /translation="SSVLDATGQVLKLYRQQFGTIPVAVSGETRPVDIAATLDSEGQT
                     VTISVVNATRDKVKIPVTLLNGTVTGEGELWQVTAPDIMATNEPGRPENVKITGPASV
                     PSAGELTVGPASINLFVFKLKN"
BASE COUNT         1006 a         1101 c         1005 g         1091 t
ORIGIN      
        1 gatctctttt ttgaattcat gatcactgaa ggggcaaaca gcggtgtgaa atacttcgtg
       61 acggaagctt atcccacgga ggggtctgcc attggcctcg agtaccagat tctggacgat
      121 cagcgtcacc cggatgcctt gctgggccgt gacggcaacc gaacggtagc ctcactctat
      181 gatctcattc ctgcgcgcaa caagcgtttc aacggcgtgg gtcagtggaa tgcagcacaa
      241 atagtatcat tgaatggtca tgtggagcac tggctgaatg gtttcaaggt gctggaatac
      301 gagaggggca gcgatgaata ccgccgcctg gtcagcgaga gcaaatacaa gattttcgaa
      361 ggcttcggcg aagcggaaca ggggcacatc ctgctccagg atcacggcga tgaggtctgg
      421 ttccggagta tcaggatcag ggagttataa gtcagtcggg gccccagtag tcagtcagca
      481 cacggtagtc agctggcggt cagtcagaag ctggaaatca gtgcacaaca atcgccatcc
      541 gggagttagg gggcacccgg caaacagcag cgggcagtca gtcagagtcc gggagtcagt
      601 aaccagcaat cagcgcatag cagccaccag ccggcaagca gtctgcagcc ggcagttagt
      661 cggcaccctg aagacgagag tcagctgccg gaaattattc aacacccggt aaaaagcagc
      721 cggcagtcac tcaaaaactg gatgactgcc ggccgttatt gtttactgta gactttacaa
      781 tgccaattac ctcactccga acttataggt agtgcttgac aggaacggct cctccggggt
      841 caggatgact gacgggaagg atgcctggtt ggggctgtca ggaaaatgct gcgtctcgag
      901 gcataatccc gagcggtact ggtaaacctt gccaccgcgg ccggtctggg tgccatcgag
      961 gaagttgccg gtgtaaagct gcacaccggg ctgatcggtg ataacctgca gaaccctgcc
     1021 ggttgacgga tcataaacct cagcatcaac ctcctcaaca ttgtcaagga cgaagttatg
     1081 gtcatagcct ctgccaagga taagctgatc atactcctca cctattctct caccaatcag
     1141 gtgcggagtg cggaaatcaa acggagtacc ttccacaggc ctcagctctc cggtgggaat
     1201 gagggtggga tcaaccggtg tgaatgccga cgccctgatg agaacctcgt ggtcaagtat
     1261 gtcaccattg cccgagccgt gcaggttgaa ataggcatga ttcgtaatgt tgatcacact
     1321 cctccggtca gtggtgacct tgtaatccat aataagctcg ttgtcatcag tccagttgta
     1381 gaccacctca gcaacaacat ttccggggta accctcttcc atgtcaggct tgttgtaggt
     1441 gaacttcaca gccggaaagt cagtgccctt cagcacttcg gcagtccaga ccctgctgtg
     1501 ccagccggca ggtccgccgt gaagggaatt tggaaaattg ttgagcgcaa gggtatactc
     1561 aacctcgtca agagtgaatt tgccctttgc aatacggttt gcatactggc ctacaacctt
     1621 cccgaaatag gcatcaccgg caagatagcc gtcaagtgtc tcgtacccga aggtaatatt
     1681 tgccttgtcg ccatttctgt caggcacatt gacctctaca atcgcagcac cataattggt
     1741 aagttttacc acatcaccct ttgcgtttgt gagtgtgaag agggatactt ccttcccatc
     1801 aaatgaaccg aaattttcag tagttaccat ctcttttgct ttgtttccgc aagagcttat
     1861 gccgaacatg aacagtacca gcactgagcc aaggaatagg tttagtttac gcatacagat
     1921 tttttttttg aaaataatgg attttatctt gaattatcaa ttcccccaat catcattttt
     1981 aaagacctgg cagggaattt ttcattgctc tcacccctgt tcacatgatc tacttcactt
     2041 caaactcaaa accaacgtgc ctgtcagatt atatgagagc tgttcacatg atctacttca
     2101 cttcaaacac gattattgac ttcgccggca tgtcaaggtt caggatattc tttttgagct
     2161 taacatcttt gaactctgca ggtttcactt cttccggctt ttcaaaggta ttatgtgtgt
     2221 taagttccgg tgctgtcagg attcttgcag atacttcttt aaaatcaccg ccgcgcaggt
     2281 cgataagcag gtttatcttt tcagacggct caatatttac cacggagata tgaaccgtgc
     2341 catcggcatc ttttgacgcc gaggcgctga tagatgccag ttccctgcca tccatggcat
     2401 aggtgccgca attgacatca agcggcagaa gggttgcatc atgatgcacc ttgtacattt
     2461 caaacacgtg gtatgtcggg gtcaggagca ttttttcacc gtcagtcagg atgagcgact
     2521 gcaacacatt taccgtctgg gcaatattgg ccatcctgat tctgtcacac ctctcgttga
     2581 aatagttgag agaaaggcct gcaaccactg catcgcgcag ggtattctgc tggaagagga
     2641 atcccggatt ggtgccaggc tccacatccc accacgtgcc ccactcatcc accacaagcg
     2701 caatccgttt tgcgggatcg tatttattca taatagtgat atgcctgtcg agcgcctttt
     2761 ccacgttcag gcatctctca agagtttcaa aatactcctt ctccccgaac tttgtagcgg
     2821 agcctttatt ctcccagttc gtggtgtagt aatgaaggga gattccccac atcaggttat
     2881 gaggcacatc gcgcatcagc acctcagtcc agttataatc ggtagagttc gctcccccgg
     2941 caattttcat gagacggtta tctccgtagt ttcttgcaaa ggttgcataa cgcctgtact
     3001 ggtcagcata aaagtcagcc gtcatatttc cgccacagcc ccagttttca tttcccacac
     3061 cccagtattt cacgttccat ggtttgtcac ggccgttggc acggcgaagg tcagccatcg
     3121 ggctgacacc gtcaaaattt atgtattcca cccactttga catctcctcc actgatccgc
     3181 tgcctacatt tccgcagata accggctcag cgccaagctg ttcgcaaaga tcaaggaact
     3241 catgcgttcc gaagctgtta tcttcagtca caccgcccca gtgcgtgttg atcattttag
     3301 gcctcttttc ccgcggtccg ataccatcca tccagtgata ctcatctgca aaacagccgc
     3361 caggccagcg gagattcgga atctgtgtcc ttttaagggc ttcaacaacg tcattgcgga
     3421 tacccctggt attaacaatg gaactcccct cacctaccca gtagccacca tagatgcaat
     3481 gtccgaggtg ctccgagaaa tggccgtaaa tgtgcctgct gattgtctgt gcccccaggt
     3541 ccgtgctaat aatgatatta gccttgcctg tcttctcccc tggctggcca aaggcagaaa
     3601 atgtcagcgc agacatgatc ataaataccg tcataatgaa gctggaatgt ctcatcacct
     3661 gttctctttt taagattatt aattatttgt attcaccaat atatcattgc taactcttat
     3721 ctgtgccccg gtgtgttggg caatctgaga ttgttttcct ttttcagata gcgttccgtt
     3781 cttttttaca taccataaga ttcagttttc accgcgtcac ctgactgtca gttctttagt
     3841 ttgaagacga acagattaat acttgccggc ccgacggtca gttcacctgc tgacggtacg
     3901 gaggccggac ctgttatctt cacattttca ggccttccgg gttcgttggt ggccatgata
     3961 tcaggtgccg tgacttgcca gagctcaccc tcaccggtaa cagtgccgtt tagcagggtg
     4021 acagggattt tcactttgtc ccgggtggca ttgacaacag agattgtcac tgtctggcct
     4081 tcgctgtcaa gcgtggctgc aatatcaaca ggcctcgttt cgccgctgac cgcaacaggt
     4141 attgtaccga actgctgcct gtaaagcttg agcacctgtc cggtggcatc aaggactgaa
     4201 ctt
//
LOCUS       DAOPBK010000194         4198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_17366_length_4198_cov_3.783732, whole genome shotgun sequence.
ACCESSION   DAOPBK010000194 DAOPBK010000000
VERSION     DAOPBK010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17366_length_4198_cov_3.783732"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..816)
                     /gene="nqrE"
                     /locus_tag="PLA17_09805"
     CDS             complement(199..816)
                     /gene="nqrE"
                     /locus_tag="PLA17_09805"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HPK85746.1"
                     /translation="MENLVNIFVRSVFIDNMIFAYFLGMCSYLAVSKTVRTSMGLGVA
                     VTFVLVITVPVNFLLEQYVLKKGALVWLSDSFATLDLSFLSFIMFIAVIASIVQLVEM
                     VIEKFSPSLYNSLGIFLPLIAVNCAILGASLFMQEREYANIGEATAFGLGSGIGWWLA
                     IVALAAIREKMRYSNVPAPLRGLGITFILTGLMGMAFMSFLGITL"
     gene            complement(828..1481)
                     /locus_tag="PLA17_09810"
     CDS             complement(828..1481)
                     /locus_tag="PLA17_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPK85747.1"
                     /translation="MGKVEKVSLLSAKNKKLLKNPLGIENPVTVQVLGICSALAVTVK
                     MKPAIVMAISVTVVVALSNMIISLIRKSIPGRIRIIVQLVVIATLVIIVDQFLKAFAF
                     DVSKQLSVFVGLIITNCILMGRLEAFAMSSDPWPSFLDGIGNGAGYGLILIIVSFFRE
                     FLGSGTVFGYPVMEKLGVYNFGYEDNGMMILPPMALIILGLIIWFQRARNKSLIETS"
     gene            complement(1484..2185)
                     /gene="nqrC"
                     /locus_tag="PLA17_09815"
     CDS             complement(1484..2185)
                     /gene="nqrC"
                     /locus_tag="PLA17_09815"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HPK85748.1"
                     /translation="MKEFSNKYIIIYSLAMGVSVAILLSLTALLLQPRQEKNVEVEKK
                     MNILSSINVLSEKSNAVQLYDKYIRDSYVINSEGKKFDGVDAFTVTLRAEQKKPLEEQ
                     YLPLFVAVDDNGDTLRIFPLEGKGLWGPIWGYISLKEDLSTIAGVTFDHKGETPGLGA
                     EINTTEFESQFHDKKLFDNGAFVSVKVVKGGAGPGNLHEVDAISGGTVTSKALEKMLL
                     DGLVKYEKYLSQKRK"
     gene            complement(2197..3354)
                     /locus_tag="PLA17_09820"
     CDS             complement(2197..3354)
                     /locus_tag="PLA17_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HPK85749.1"
                     /translation="MSFLLDKMKKIEPLFKKGGRFEKLYSFYEGFASFLFVPDKVTTK
                     GSHIRDHMDMKRTMTVVIIALMPALLFGCYNIGLQHTRAFGLNDWPVIQMFLFGFIKL
                     LPMIIVSYLVGLGIEFIAAQIRGHEISEGYLVTGMLIPLIMPVDVPLWMLAISVAFAV
                     ILGKEVFGGTGMNILNPALTARAFLFFSYPGRMSGDKVWIEGLTKGGGPDGFSGATPL
                     ALARLDGFDQMPSPMEMFVGTIPGSVGETSVVAILIGAFILLLTGVGSWRIMLSMTLG
                     ALGMGLLLNAVGGNEYTQIPAYYHLIMGGFAFGTVFMATDPVTAAQTNTGKYIYGALT
                     GIMVVIIRVFNPAYPEGTMLAILLMNVMAPLIDHYVVNASIRRRLKRGRRV"
     gene            complement(3374..>4198)
                     /gene="nqrA"
                     /locus_tag="PLA17_09825"
     CDS             complement(3374..>4198)
                     /gene="nqrA"
                     /locus_tag="PLA17_09825"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HPK85750.1"
                     /translation="TGIDALSRLTDGKVHLSQDAEGTRVAEIKNLRNVEFHRFSGPHP
                     AGNVGVQIHHIAPVCRGEVVWTLDVQDVVAIGRLFATGFYDRERIIALAGPGVLNRKY
                     HRTRPGASVAGILKGKLAEGNLRVISGNVLTGKKISTEGSLGFYDNMITVIPEGDYHE
                     FMGWAAPGLKKYSYWRAFVSKLLPKKEYILDTNLHGGHRAFVVTGDYENVLPMDIYPV
                     YLLKAILAGDIELMENLGIYEVAEEDFALCEYIDPSKTEIQEIIRSGINLMIKEMN"
BASE COUNT         1085 a         1178 c          940 g          995 t
ORIGIN      
        1 cgacagcaga ctcgatcccg gtgagacaac catctcacgg tcattgaccc ggagagtgac
       61 atcgccccgg ggcatgagct tctgcctggc atacagcagc aacaccacaa gcagtatcaa
      121 caccagtaca aatactgcta tactcatgag aactgtgccg gcaattgata atcctaaaat
      181 catttccttg caatttcttt atagcgtaat ccccaggaag ctcataaaag ccatccccat
      241 cagtcccgtt aatatgaaag tgatccccag tccgcgcaac ggtgcaggca catttgaata
      301 gcgcatcttc tcccttatcg cggccagcgc aacaattgcc agccaccagc ctatgcccga
      361 accaagtccg aaagcagtgg cttcgccaat gtttgcatac tccctctcct gcatgaagag
      421 tgaggctccg agaattgcgc agttcacggc aataagcgga agaaagatac ccagggagtt
      481 atagagcgag gggctgaatt tctcaattac catctcaaca agctgcacaa tggatgctat
      541 gaccgcaata aacatgatga acgaaaggaa actgagatcc agggtggcaa agctgtcact
      601 cagccacacc agcgctccct tcttcagaac atactgctca agcaggaagt tgacggggac
      661 ggttatcacc agcacgaaag tcactgcaac gcccagcccc atggatgtcc tgacagtctt
      721 cgacacagcc aggtatgaac acatacccag gaagtatgcg aagatcatgt tgtcgataaa
      781 gaccgatctg acgaaaatat tgactaagtt ttccatgacc actgctctta gctggtttca
      841 atcaatgatt tgttgcgtgc acgctggaac cagattatca gaccaaggat tataagcgcc
      901 atcggcggaa gaatcatcat gccgttatcc tcataaccga agttgtaaac tcccagtttc
      961 tccattacag gatagccaaa aacagttccg ctgcccagga actcacggaa gaatgagaca
     1021 attatcagta tcaggccata cccggcgcca ttgcctatgc catcaaggaa agaaggccag
     1081 ggatcggaag acatggcgaa agcctccagg cgtcccatca ggatacagtt ggtgatgatc
     1141 agccccacga agaccgagag ctgtttgctc acgtcaaagg cgaaggcctt caggaactgg
     1201 tcgacaatga ttaccagagt ggctatgacc accagctgga ctatgatcct aatacgtccg
     1261 ggtattgatt tgcgtatcag cgaaataatc atgtttgaca gtgccaccac aaccgtaacg
     1321 gatattgcca ttacaattgc gggcttcatc ttcaccgtca ctgccagagc cgagcagata
     1381 cccagtacct gcactgtgac agggttttca atcccaagcg ggtttttcag cagtttcttg
     1441 ttctttgccg acaacagcga caccttttca acttttccca tatctacttc ctcttttggc
     1501 tgaggtattt ttcatattta acaaggccgt caagcagcat tttttccaat gctttactgg
     1561 ttacggttcc gcccgagatg gcatccacct catgcaggtt gcccggtccg gcaccgcctt
     1621 tcaccacttt tacagagaca aatgcgccat tatcaaagag tttcttgtca tgaaactggc
     1681 tttcaaactc cgtggtattt atctctgcgc caagacctgg cgtttcaccc ttatggtcaa
     1741 aggttacacc cgctatggta gacagatcct ccttcagtga aatgtatccc catatcggac
     1801 cccacaaccc tttcccctcc agggggaaga tacgaagggt atcaccattg tcatccaccg
     1861 ccacaaagag aggcaggtac tgttcttcca gcggtttttt ctgctcggcg cgaagggtca
     1921 cagtaaaggc atccactcca tcgaacttct ttccttcact gtttatcacg taactgtccc
     1981 tgatatactt gtcataaagc tggacggcat ttgacttttc agaaagcaca ttaatggagg
     2041 agaggatatt catcttcttc tccacctcca cgttcttctc ctgtctcggc tgaagcagca
     2101 aagccgtcag cgacaggagg atggcgacgg agacgcccat cgccagcgaa taaataatta
     2161 tatacttgtt gctgaattct ttcatatcat gtgcagttaa actctccttc ccctttttaa
     2221 ccttctcctg atactggcat taaccacata gtggtcaatc agcggtgcca taacgttcat
     2281 cagcagtatc gccagcattg ttccttcggg atatgccgga ttgaagaccc tgattatcac
     2341 caccatgatt cctgtcagcg caccatatat atatttcccg gtattggtct gagcggcagt
     2401 aaccgggtct gttgccataa agaccgttcc gaatgcaaat ccgcccatta tcaggtggta
     2461 gtatgcgggt atctgtgtgt attcgtttcc gccaacagcg ttaagaagca ggcccatacc
     2521 cagcgccccc agggtcatgc tcagcatgat ccgccagctg cccacaccgg taagcagcag
     2581 aatgaatgcg cctatgagta tcgccaccac agaggtctct cccactgacc ccggaattgt
     2641 tcccacaaac atctccatgg gtgaaggcat ctggtcaaat ccgtcaagcc tggccagtgc
     2701 cagcggcgtg gctcccgaga agccgtcagg gccaccgcct ttggtcagac cctctatcca
     2761 taccttgtca ccgctcatcc tgccggggta gctgaaaaag agaaaagccc tggctgtcag
     2821 cgccgggtta agtatgttca tgccggtgcc gccaaaaacc tccttaccaa ggattacggc
     2881 aaaagccaca gagatggcca gcatccacaa cggcacatct acaggcataa tgagaggtat
     2941 gagcatccct gtcaccaggt agccctcgct gatctcatga cccctgatct gtgcggcaat
     3001 gaactcgata cccagcccaa ccaggtagct cacgattatc atcggcaaca gcttaatgaa
     3061 cccgaacagg aacatctgaa tcacgggcca gtcattgaga ccgaatgccc ttgtatgctg
     3121 aagtccgata ttatagcagc cgaacagcag ggcaggcatc agcgcgatga tcacgacggt
     3181 catcgtccgc ttcatgtcca tatggtccct gatatgcgac cccttcgtgg tgaccttgtc
     3241 aggaacaaaa aggaaggaag caaagccctc atagaaggaa tacagcttct caaaccggcc
     3301 tcccttctta aagagaggct ctatcttctt catcttatcc agcaggaaac tcatggcaga
     3361 ctctctcctt aatttagttc atctctttaa tcatcaggtt gatcccgctg cggattatct
     3421 cctgtatctc tgtcttggaa gggtcaatgt actcgcacag ggcgaagtcc tcctcagcta
     3481 cctcgtaaat gcccagattc tccataagtt cgatatcgcc ggcaagaatg gccttgagaa
     3541 gatagacggg atatatgtcc atgggaagga cgttctcata atctcctgtt acaacaaatg
     3601 ccctgtggcc gccgtgaagg ttggtgtcaa ggatatactc cttcttcggc aggagcttcg
     3661 aaacaaatgc tctccagtaa ctgtacttct tcagtcccgg agcagcccag cccatgaact
     3721 catgatagtc tccctcggga attactgtaa tcatgttgtc atagaaaccg agagagcctt
     3781 ccgttgatat cttcttgccg gtaagcacgt tgccgctgat gaccctgaga ttgccttcag
     3841 ccagtttacc tttaaggatt ccggccactg aagctccggg cctggtacgg tgatacttgc
     3901 gattaagcac acccgggccg gccagtgcga tgatacgctc gcggtcataa aagcctgttg
     3961 caaacaacct gccaatcgca acgacatcct gcacgtcaag cgtccagaca acctctcccc
     4021 tgcacacggg agcaatatga tgtatctgca cacccacatt gccagccgga tgcggacctg
     4081 aaaagcggtg gaattccaca ttcctgaggt tttttatctc tgccaccctt gttccttcgg
     4141 catcctgcga aagatgcacc ttgccgtcag taagacgtga gagggcatcg atcccggt
//
LOCUS       DAOPBK010000195         4183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_17430_length_4183_cov_5.124516, whole genome shotgun sequence.
ACCESSION   DAOPBK010000195 DAOPBK010000000
VERSION     DAOPBK010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17430_length_4183_cov_5.124516"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1056
                     /gene="pgl"
                     /locus_tag="PLA17_09830"
     CDS             325..1056
                     /gene="pgl"
                     /locus_tag="PLA17_09830"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPK85751.1"
                     /translation="MSHSVRVFSSLEALAQSIAAILIKDLDRIESDHYYTLALSGGSA
                     AEYIFSYLSDNYADRISWEKLLVFWVDERCVSPDDDESNYRMAYESLLQNVPIPDANI
                     FRIRGEDDPSAEARQYSELVTSMVPEYRGIPQFDLMLLGLGGDGHIASIFHDRPDLFT
                     TGRLFEVVTRPDKKQKRITATGRLINNSLKVYFIVVGKEKATVVSQVIEGKSGSELYP
                     ATRIMPASGHLVWMLDTGAASLLKS"
     gene            complement(1174..2283)
                     /locus_tag="PLA17_09835"
     CDS             complement(1174..2283)
                     /locus_tag="PLA17_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPK85752.1"
                     /translation="MKRFYLFIIVAFLSLSLFSQDFFRGDFSKDKKYVIIVNPTTDNV
                     AMVTFLMVQGILKTDPGLVEFVGVYHSSQEYDFSKSAEFISANGLTNFHLHEVNAPLD
                     EEVVYKQNQCSSDFRKIFSNSVGIIFFGGPDIPPALYGQENLYSLTNDPGRHYFEVSF
                     LFHLLGGTRDSDYKPLLEENPDYMITGFCLGLQSMNVATGGTLWQDIPAQLYQSTTSE
                     TNVQIDRRNLHRNYWQEIRDDKDLMPVSLHPVKFTEEMFFGNRVKVSRELEPIVCSIH
                     HQAINELSPAFSATATSYDGKIVEGIAHKKYPNVFSVQFHPEIPDLYMETALLRFAPE
                     DTPATVHSILGKESQEFHIKYWEFISGVIGEQASK"
     gene            complement(2471..>4183)
                     /gene="uvrA"
                     /locus_tag="PLA17_09840"
     CDS             complement(2471..>4183)
                     /gene="uvrA"
                     /locus_tag="PLA17_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPK85753.1"
                     /translation="GINRFFEHLEEKKYKIQYRVMLSRYRGKRLCPECGGMRLRKEAG
                     YVKVGGRSVTEVTSIPISEAHSFFGQLVLNGEEKEIAGRLLKEIQQRLSFLVEVGLSY
                     LTLDRLSNTLSGGESQRINLATSLGSSLAGSLYILDEPSIGLHPRDTAMLVNALKELR
                     DLGNTVIVVEHEEEIIRAADQIIDMGPAAGTHGGEIIYQGTFRFPKKNNSLTAAYMTG
                     ALNIETPAMRRKWHSYIAVRGARENNLKDIDVRFPLRTITAVTGVSGSGKSTLVHDIL
                     FNALSSRLKGNGVRAGRFTALEGDINSITAVEMIDQDPIGKSSRSNPVTYLKAFDEIR
                     KLFAGQQASVQAGFKPAHFSFNTDGGRCEECQGEGVIRVEMQFMADVELTCEACKGMR
                     YKRDILDIRFDGKNIYDVLEMTIEEAIEFFGTSKDKTCQRIAAKLKPLADVGLGYVRL
                     GQSSSTLSGGESQRVKLAFYLSQEKSTERMLFIFDEPTTGLHFHDISKLMKSLNALVE
                     RGHSVILIEHNVEVIKCSDWVIDLGPEGGEKGGYVVFEGTPEQLAQCDESYTAKFLKK
                     KLNR"
BASE COUNT          977 a         1015 c         1056 g         1135 t
ORIGIN      
        1 tttttttcat aaacccactg tcaaagaatt cacaggaaag caatatgatc ctttttcctt
       61 ccaatgtgcg gggatgagca acagttactc cttactgtct caggttaaat ccttgaattt
      121 tgttgcagag agtcacccag tcaaggctga gcagccctga ctgggggggg gatatgttcc
      181 ggggcacacg cagcagaatc aggagtttaa ttagatcatt gtcagagaag ggtcaaattt
      241 ttatatttgc tttactattc ctgcagatga tgcagtttgt tgtaaattat aattagtttt
      301 atacaagatt actggttatt tgtcatgtca cattcagtca gagtgttttc ctctcttgag
      361 gcactggctc aaagcatcgc ggcaatactg atcaaggatc tcgatcgtat agagtctgat
      421 cactattata cacttgctct ctcaggaggc tctgccgctg aatacatctt tagttatctt
      481 tcagacaact atgcagaccg aataagctgg gaaaagctgc tggtcttctg ggttgatgag
      541 agatgtgtct ctccggatga cgatgagagc aattacagga tggcctatga aagcctcctg
      601 cagaacgttc cgatacctga tgcaaatatt ttcaggatac ggggcgagga tgacccctct
      661 gcagaggccc ggcaatattc agagcttgtg accagcatgg tgcctgaata caggggcatc
      721 ccgcagtttg acctgatgct gctgggcctg ggcggcgacg gtcacatagc ttctatcttt
      781 catgacagac cggacctgtt cactacaggt cgtctgttcg aggtggtcac cagacccgat
      841 aagaagcaaa agagaattac agctacaggg aggctgataa acaattcatt aaaagtttac
      901 ttcattgttg tcggtaagga gaaagcaaca gttgtatcac aggttatcga aggcaagagc
      961 ggctcagaac tctatccggc aacccggata atgcctgcct cggggcacct ggtatggatg
     1021 cttgataccg gagcagcctc actccttaag agctgaaata aaattgtcac tttccggctg
     1081 tgattgcagc tgttctcaga gaagcagagg gtgaaaaaaa gtcgcctttc tcccggtgag
     1141 aaacagctgc attggtcaat gccgggtctc tctttattta ctggcctgtt ccccgataac
     1201 gccggagatg aactcccagt atttaatatg gaactcctgg ctctctttgc caagtatcga
     1261 gtgcacggtg gcaggcgtgt cttcaggagc aaatctcagc agtgcagttt ccatgtagag
     1321 gtcaggtatt tccgggtgaa actgcacgga gaagacgttt ggatactttt tatgtgcaat
     1381 tccctcgact attttgccat catatgacgt tgcagtcgca ctgaatgccg gcgacagttc
     1441 atttattgcc tgatgatgga tgctgcagac tattggctcc agttcgcgtg aaacctttac
     1501 tctgttcccg aagaacatct cttcagtaaa cttcacggga tgtaagctta cgggcatgag
     1561 gtccttgtca tccctgatct cctgccagta gttgcggtgc aggttcctgc ggtcaatctg
     1621 cacattcgtt tcagacgttg tgctttggta gagctgtgcc gggatatcct gccacagtgt
     1681 tcctccggtt gcaacgttca tcgattgcag acccaggcag aaaccggtga tcatatagtc
     1741 cggattctcc tcaagaaggg gtttgtaatc agaatcacgt gtgccaccca acaggtgaaa
     1801 gaggaagctc acctcgaagt agtgtcttcc cggatcgttg gtcaaggaat agaggttctc
     1861 ctgcccgtaa agagccggag ggatatccgg accgccaaag aagatgattc ccactgaatt
     1921 gctgaaaatc ttccggaagt cggaactgca ttgattctgc ttgtaaacca cctcttcgtc
     1981 aagtggtgcg ttcacttcat gcaggtgaaa gttggtcagt ccgttggcag aaataaactc
     2041 cgctgatttg ctgaagtcgt attcctggct ggagtgatat accccaacga attcaaccag
     2101 tccgggatcg gttttcagga tgccctgaac catcaggaat gtcaccatgg caacattgtc
     2161 agtggtggga ttgacaatga tgacatactt tttatccttg ctgaaatcac ccctgaagaa
     2221 atcctgcgaa aagagggaga gtgacaggaa ggcaacaatg ataaacagat aaaacctttt
     2281 catagcgttg agttatttat atacaaatgt aatgtttatt ttttgaatgc aatcgttttc
     2341 acatgaaaga gaaaaccggt ttttagcagg acttttttca cggatgcagt aacaacactg
     2401 gtttggtcag gaggttggga gatcttccga aagccacttt ttacagagtg cagtaacatc
     2461 attggcttgg tcagcggttg agttttttct tcagaaattt tgccgtgtac gattcatcgc
     2521 actgtgccag ctgttcaggt gtgccctcga acacaacata cccgcccttt tcaccaccct
     2581 cagggccaag gtcaatcacc cagtcagagc attttatcac ttccacgttg tgttctatca
     2641 gtatcaccga atgcccgcgc tcaaccagtg cattgagtga cttcatcagt ttgctgatat
     2701 catgaaagtg gaggcctgtg gtgggttcgt caaagatgaa aagcattctt tcggtgctct
     2761 tttcctggct gaggtagaag gccagtttca ccctctggct ctcgccaccg gagagggttg
     2821 aagacgattg tccgagcctg acgtacccga gtccgacatc agcaagcggt tttagtttcg
     2881 cggcaatcct ctggcaggtt ttgtctttgc ttgtgccgaa aaactcaata gcctcctcga
     2941 tggtcatttc gagaacatcg taaatatttt tcccgtcgaa tctgatgtca aggatatcgc
     3001 gtttatagcg catccccttg catgcctcgc atgtaagctc aacgtcggcc atgaactgca
     3061 tttctaccct gatgactcct tcgccctggc attcctcgca acggcctcca tcagtattga
     3121 aagagaagtg ggctggtttg aatcctgcct gaactgaggc ctgctgcccg gcgaaaagct
     3181 tgcggatctc atcaaaggct ttcagatagg ttaccgggtt tgaccggctt gatttgccga
     3241 tagggtcctg gtctatcatc tccacggctg tgatgctgtt tatatctccc tccagcgcgg
     3301 tgaaccggcc ggctctcacg ccgttgcctt tcagccgcga ggagagtgcg ttgaacagga
     3361 tatcgtgaac gagtgtgctt ttgcctgatc cgctgactcc ggtcactgcg gtgatcgtcc
     3421 gcagggggaa cctgacgtca atgtccttca ggttgttctc gcgcgcgccg cgtaccgcta
     3481 tgtaactgtg ccatttgcgg cgcatggcag gtgtctctat gttgagtgct cctgtcatgt
     3541 aggcagctgt cagtgagttg ttcttcttcg ggaacctgaa ggtgccctgg tagataatct
     3601 cacctccatg ggtgccggca gcaggtccca tgtcaataat ctggtctgcg gccctgatga
     3661 tctcctcttc gtgttccacg acaatgaccg tgttgccaag gtcacgcagt tccttcagtg
     3721 cattgacaag catcgcggtg tcacgagggt ggagtccgat actgggttcg tcgaggatat
     3781 aaagagagcc tgccaggctg cttccgagag aggttgccag gttgattcgt tgcgattcac
     3841 ctcccgagag ggtgttggaa agcctgtcga gggtaagata ggagagcccc acctctacga
     3901 ggaaactcag ccgttgctgt atctccttca gaaggcggcc tgcaatctcc ttctcctcgc
     3961 cgttgagcac cagttgcccg aagaagctgt gtgcttcact gatggggatg gaggtgacct
     4021 ctgtaactga cctgccacca accttaacat agcccgcctc cttgcgcagg cgcataccgc
     4081 cacattcagg gcataagcgt ttgccacggt agcggctcag catgaccctg tactgtatct
     4141 tgtacttctt ctcttcaaga tgttcaaaga acctgtttat acc
//
LOCUS       DAOPBK010000196         4173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_17475_length_4173_cov_4.060952, whole genome shotgun sequence.
ACCESSION   DAOPBK010000196 DAOPBK010000000
VERSION     DAOPBK010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17475_length_4173_cov_4.060952"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="PLA17_09845"
     CDS             complement(<1..1484)
                     /locus_tag="PLA17_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPK85754.1"
                     /translation="MRLEQRATAVLAAIMLLLNLPDADSQANAERRAGDKPGIKMSCQ
                     PSDFIYRSGESKFQTITDPSGKYLRLFLPGHHQSHETGQPELPVWSQLVEVPDGMEVV
                     VSISEVTSRRIRFADHGAAQAELFPAQPARTKNQVRDNRITVKDRKAYGSQNILMHDT
                     VKVTHEGIFRGRRLANITVSPAFYNPGGRYVDLITSMKVVIGFKPEATKGEEQPEDPV
                     NKGGFASDSYITGYTDQPVHMIIVTDSIFRKHLEPLVKWKALKGIRTTVIYKKSGPSD
                     SIYKDLKNRISAVYFDLQEKGIPVHYLMIAGDQSIIPTSQGTTFVSDLYYGEFDGGGD
                     YIPELFIGRLPASDTTQMKGMVRKIIDYETNRLGPAVDNWSRALVTAGNAPGYELYMN
                     GQVSYIYNTYLKTDTSLDAIGWLYPDSPQKDDSLKIVFNKGVSILNYTGHGEATGFSD
                     PAFRTSMMNEFSNENKYPLIIANACRTAQINVPSCFGTAMVATPG"
     gene            1753..>4173
                     /locus_tag="PLA17_09850"
     CDS             1753..>4173
                     /locus_tag="PLA17_09850"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HPK85755.1"
                     /translation="MDKSGVMYFGNESGGIVTYNGSRWGKINTPGIGAVTTLITDTHG
                     IVYAGGSRGFGFLQPDNTGRMVYVSLAGLTGSGEEIYSPITSSTADSNYVYFSDGRRL
                     FVKDPKKDSVSVIDLKRELGIKDVSALLAFDNRLIIADNQKGLFARSADRTGRIPGGE
                     KVAPAGFIRLLPYDRDNLLIGDAQNGLMLFNIRTGAIAFLPADTKSKSVVQRGSLTDV
                     ALLPGNLIAAGYSDRGGVYIYSHEGTLLQHISERTAAVTESSVTAMYCNNASNSQLWF
                     CTSGFINRASISLPASGFTPAAGITSVCGPVAEYAGEIYAGTENGLLVSYTDPSGERR
                     FRIKGGQGARVYSLAPVDLPEGRVLLAATENGLLQLDSYDDLERFLPGLHLTAARNSG
                     MNPGIMISGSPDGTVRTLVHDGYEWEIRHTISNAVTGRISDIEQTTPGDWWLLTSDPV
                     SLVRMRCESNDTSFVVYGRDKGVISDTLNSLAVINNNLYLCTGRGISVYDGQDDTFRK
                     DHDLTGKTFDEANIRILISAPEGEVIISGYDARNFDAIVATTRQGHVVFRRQFDFLPD
                     LATTGISYADGSVWLAKGQNLFVMDKSKLAFRYGDFSTLFTRISTGSGHILMDGTFYT
                     DSKEGQRIPSAIQPARPGITISHRDNNLTFNWTTTSYIDEEKTEYRHRLEGYDDDWSS
                     WEKRTCRDYTNLPSGDYRFVVKARTITGFEGEEISYSFSVKRPWLSSLVAKLFYIVIA
                     GWLLFLLMRYSARRLRIRNKRLDNLLRQRNEAAAKGRNEIEGLEKYAGLVQQTLLHSE
                     RRLGEAIPN"
BASE COUNT         1071 a          979 c         1077 g         1046 t
ORIGIN      
        1 cccggggtgg caaccatggc tgtgccaaag cacgaaggaa cgtttatctg ggctgtgcgg
       61 catgcattgg cgatgatgag agggtacttg ttttcattgc tgaactcgtt catcatcgag
      121 gttctgaaag cagggtcact gaagccggtg gcttcaccat gaccggtata gttcagtatg
      181 ctgactccct tgttgaaaac aatcttcagc gagtcatcct tctgaggcga gtcaggatag
      241 agccagccga tggcatcaag ggatgtgtct gttttcaggt aagtattgta gatgtatgac
      301 acctgcccgt tcatatacag ctcatatccc ggcgcattgc ctgctgtgac gagtgcccgt
      361 gaccagttgt caacagcggg gccaagcctg ttggtctcgt aatcgataat tttcctgacc
      421 atccccttca tctgggtggt atctgatgcc gggagcctgc caatgaatag ctcagggata
      481 tagtcgcccc cgccatcaaa ttcgccgtag taaagatctg acacaaaggt ggttccttgt
      541 gatgtgggta ttatggactg atcgccggcg atcatcaggt aatgtaccgg aatccccttt
      601 tcctgaagat caaaataaac ggcagatatc ctgtttttca gatccttgta tattgaatca
      661 gaaggtcctg atttcttgta aattacagtt gtacgtattc ccttcagcgc tttccatttg
      721 accagtggtt ccagatgttt tctgaaaatg gagtcggtga ctatgatcat gtgaacaggc
      781 tggtcagtat agcctgtaat ataggagtct gaagcaaatc cgcccttgtt gaccggatct
      841 tccggttgtt cctcaccctt tgttgcttcc ggtttgaacc cgatcaccac cttcattgac
      901 gtgataagat cgacgtatct gccccctgga ttgtagaaag ccggtgagac tgtgatattt
      961 gccagccggc ggcccctgaa tatgccttcg tgagtcacct ttaccgtatc atgcatcagg
     1021 atgttctgac tgccataggc tttccggtcc ttgacggtga ttctgttatc gcgtacctga
     1081 ttcttggttc ttgccggctg tgccgggaaa agctcggctt gggcggctcc atggtcagca
     1141 aaacgaattc tgcgcgaagt cacttccgag atggaaacaa ccacctccat tccatccggt
     1201 acttcaacaa gctggctcca taccggcagc tccggctgtc cggtctcgtg agactgatgg
     1261 tgccccggta gaaacagcct caggtacttg cctgacgggt cggtaatggt ctgaaatttt
     1321 gattcacctg aacggtagat gaagtcagac ggctgacacg acatttttat gccaggtttg
     1381 tcgccagccc tcctttcggc attagcctgg gaatcagcat cgggcaggtt tagcagaagc
     1441 ataatagccg ccaacactgc agttgccctc tgttctaacc tcataacctg aatatatgaa
     1501 gccaagataa ctaaaaatta ccttaatata tatcccggaa gtgatttcaa ccgttatata
     1561 aattgtatta gtatatttaa atataaatta agggaaattg ttcagggtat gaagaaggga
     1621 ttcaaaatta cgatactcct tctattatta tcattatcag tattttcgca ggtcaacagg
     1681 tatggtaccc ctttgatcag ccggtttgat gcatccgcta ctccgggaga gctccgaaat
     1741 ctgtgtatca caatggacaa aagcggggtg atgtattttg gcaatgaatc cggaggaatt
     1801 gtcacatata acggcagcag gtgggggaag ataaacaccc cgggtatcgg agcagttacc
     1861 acactgatca ctgacacaca tggaattgta tatgccggag gaagcagggg attcggtttc
     1921 ctgcaacctg acaatacagg cagaatggtc tatgtttcac tggcagggct tacaggaagc
     1981 ggggaagaga tctacagccc cataacctca agtactgcag acagtaatta tgtctatttc
     2041 agtgatggca ggagactgtt tgtcaaagat cctaaaaagg attcagtttc agttatcgac
     2101 ctgaagaggg aattggggat aaaagatgtt tctgcgttgc ttgcatttga caacaggctg
     2161 ataattgctg acaaccagaa gggattattt gcccgcagcg ctgacagaac cggcagaata
     2221 ccaggaggag agaaagtggc acctgccgga tttatacggc ttcttccata tgacagagac
     2281 aatcttctca ttggcgatgc acaaaatgga ctgatgctgt tcaacatcag aacaggcgct
     2341 atcgcttttc ttccggcaga cacgaaatca aagagcgtgg tgcaaagagg ttcactcacc
     2401 gatgttgccc tgcttcccgg caacctgatt gctgctggtt attcggacag gggaggggtc
     2461 tacatatata gtcatgaggg aacgcttctc cagcatatat cagaaagaac ggcggcagtg
     2521 actgagtctt cagtgacagc catgtactgc aacaatgcat ccaattcaca actgtggttc
     2581 tgtaccagcg gatttataaa cagggcttcc atatcattgc cggcatccgg gttcacaccc
     2641 gccgccggta tcacttctgt ctgcggtccg gtagcagaat atgccgggga aatatacgcc
     2701 ggaactgaaa atggccttct tgtcagttac actgatccgt caggtgagag gagattcagg
     2761 ataaaagggg gccagggagc cagggtctat tcacttgccc ccgtggacct cccggaagga
     2821 agagtgctgc tggctgccac agaaaacgga ctcctgcaac ttgacagtta tgacgatctg
     2881 gaacggtttc ttcccgggct gcacctcact gctgcccgca actcgggcat gaatcccggg
     2941 ataatgatat caggctcacc cgacggaaca gtcagaacgt tggttcatga tggctacgaa
     3001 tgggaaatca ggcacaccat cagcaacgcg gttacaggtc gcatcagtga tattgaacag
     3061 accacaccgg gtgactggtg gctgttaaca tcagatccgg tatcactggt aagaatgcgc
     3121 tgtgagtcaa atgacacgtc atttgtagtt tatggcaggg ataagggagt gatatctgac
     3181 accctgaaca gccttgccgt cattaataac aatctttacc tgtgcacagg caggggtatc
     3241 tctgtttatg acgggcagga tgatactttc aggaaggatc atgatctgac aggaaagact
     3301 tttgatgagg ccaatatcag gattctgatc agcgcacctg aaggggaagt gatcatatca
     3361 ggctatgacg cgcgcaactt tgatgctata gtcgcaacca cccgccaggg tcatgtggtc
     3421 ttcagacgtc agtttgattt cctgcctgac ctggcaacaa cgggaatatc ctatgctgac
     3481 ggaagcgtct ggcttgcaaa ggggcagaac ctctttgtaa tggataagtc aaagcttgcg
     3541 ttcaggtacg gtgatttcag tactctgttt accaggatca gcaccggaag cggacatatc
     3601 ctcatggatg gcaccttcta taccgattcg aaggaggggc aacggatacc ctctgcaata
     3661 cagcctgcca gacctggcat caccatttcc cacagagaca acaaccttac ctttaactgg
     3721 acaaccacct cctatattga tgaggagaag actgagtaca ggcaccgcct ggagggttat
     3781 gatgatgact ggtcatcctg ggaaaagcgc acctgcaggg attacaccaa tctgccctcc
     3841 ggtgattacc ggtttgttgt aaaggcaaga acaataacag ggtttgaggg agaggagatt
     3901 tcctattcat tttctgtcaa gaggccgtgg ctgagctccc ttgtggcaaa acttttctac
     3961 atagtcattg ccggctggct cctgttcctc ctgatgagat actctgcaag gaggctgcgc
     4021 ataaggaaca agaggcttga caacctgctc aggcaaagga atgaagctgc ggcaaaaggg
     4081 aggaacgaga tagaagggct tgaaaaatat gccggcctgg ttcagcagac tctgctgcat
     4141 tcagagagga ggctgggaga ggccatcccg aac
//
LOCUS       DAOPBK010000197         4167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_17511_length_4167_cov_5.340467, whole genome shotgun sequence.
ACCESSION   DAOPBK010000197 DAOPBK010000000
VERSION     DAOPBK010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17511_length_4167_cov_5.340467"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PLA17_09855"
     CDS             complement(<1..930)
                     /locus_tag="PLA17_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPK85756.1"
                     /translation="MSLEKKISELREKREKMLLGGGEEAIVKQKSMEKNTARERIMAI
                     LDAGSFNEYDMFVEHDARDFGMDKKILPGDGVVIGTGTIHGNPVAIFAQDFTVAGGSL
                     GLMHSRKITKIMDYALNMRIPLIGINDSGGARIQEGVNSLAGYGEIFFRNTISSGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDNISKMFITGPEVIKTVLGEEISMEALGGARV
                     HCETSGNAHFFSSSEEECFNQIRRLISFIPGNNEMKAAVTETLPPEAGYEIEKIVPAD
                     PTLPYDVREVIRAVADNSDFFEIQEMFARNMVIG"
     gene            complement(1027..2166)
                     /locus_tag="PLA17_09860"
     CDS             complement(1027..2166)
                     /locus_tag="PLA17_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPK85757.1"
                     /translation="MEGRDSSENIRKSLKKLLPGILDWYIIRQFLGTFLFSLVLILGI
                     AVIFDFSEKIDDFIQKQAPTHAIIFDYYLNFIPYFATLFAPMFVFISVIFFTSRMAVN
                     TEIIAILNGGMSFRRLMVPYLISAAFIAFCAFLLQNSLLPHANAARLDFEEKYYRSSD
                     FRRVNVSSVHRQVYPNVYIYMEQYNRISQSGRNFSMEKFNDQGMLESKLTANMIRWDS
                     TTGKWGAWWYYIRETDSLGERISSGRRIDTVLNVGPDDFTRSPEFVFTMTNSELLDYI
                     QVLKAQGSDEIKLYLNEKHRRYSSPFAFFILTIIGVSLSSRKIKGGIGMQIGIGLVLT
                     FSYILFMQFSSQFSLKGDLNPALAMWIPNIIYTFIAAGVYYTAPK"
     gene            complement(2124..3251)
                     /gene="tgt"
                     /locus_tag="PLA17_09865"
     CDS             complement(2124..3251)
                     /gene="tgt"
                     /locus_tag="PLA17_09865"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPK85758.1"
                     /translation="MFTIESRDGTTSARAGLLRTAHGDIPTPVFMPVGTAANVKGVFH
                     RDLKQEIDAPVILANTYHLYLRPGTEIIRLAGGVHRFMAWDRAVLTDSGGYQVFSLAA
                     NRKLTEEGAIFRSHIDGSKHLFTPEGVIDIQRQIGADIIMAFDECAPYPCDHGYALKS
                     MEMTHRWLDRCVARMASTEPLYGHEQMLFPIVQGSVYGDLRRLSAEKCASAEMAGNAI
                     GGLSVGEPAEVMYEMIEVVNKILPADKPRYLMGVGTPANILEGIARGIDMFDCVMPTR
                     NARNGMLFTRDGIVNIRNQKWATDFSPIDAASHSEISRTYTRAYLRHLVISGEMLGAQ
                     IATIHNLAFYLSLVREARERILDGTFGSWKEETVRKISERA"
     gene            3406..>4167
                     /locus_tag="PLA17_09870"
     CDS             3406..>4167
                     /locus_tag="PLA17_09870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK85759.1"
                     /translation="MIISTDTTHLILLGLLLTMFIIQAWYWLGYYRRTAFAKVPEIAP
                     GTPFPPLSVVICARNEAENLSRFLPDVLNQDYPSFEVVVVNDCSEDNSFDILGGLMTR
                     YPHLKVSMIQKDPRFKHTKKLAMLIGIKAAKNDLLVFTDADCRPASPQWLRHVASAAT
                     GEKSDIVLGYGAYSEEKGLLNSYIRYETMFIAMQYFGMAMAGKPYMGVGRNLSYRRTF
                     FFGQSGFGPHNHIISGDDDLFVNRTSTSENCSLMLA"
BASE COUNT         1041 a         1106 c          988 g         1032 t
ORIGIN      
        1 gccgatgacc atgttccggg caaacatctc ctgtatctca aagaaatcgg aattgtcagc
       61 aaccgccctg ataacctccc tgacatcata gggaagcgtc gggtcagcag gcactatctt
      121 ctcaatttca tacccggcct caggcggcag tgtttccgta acggcagcct tcatctcatt
      181 gttcccgggg ataaaggaga tcagcctcct gatctggttg aagcactcct cctcagagga
      241 tgaaaagaaa tgggcattgc ccgatgtttc acagtggacg cgggcgcctc ccagcgcctc
      301 catcgatatc tcctcaccca gaacggtctt tatcacttca gggccggtga tgaacatctt
      361 tgaaatattg tcaaccacaa aaacgaagtc ggtcagggcc ggcgaatata ccgcacctcc
      421 cgcgcagggt cccagtatca ccgatatctg cggaatcacc ccgctggaga tggtgttcct
      481 gaagaaaatc tcaccatagc cggcaagtga attaactccc tcctggatgc gggcaccacc
      541 cgagtcattt atccctatga gtggtattct catattcagg gcataatcca tgatcttggt
      601 gatttttcgc gaatgcatca ggccgagcga cccccccgca acagtaaaat cctgggcaaa
      661 aatcgcgaca ggattgccgt gaatggttcc ggtgcctatc accacaccgt cgccgggaag
      721 aatcttttta tccattccga aatcacgggc atcatgctcc acgaacatgt catattcatt
      781 gaagctgccg gcatcaagga tggccataat gcgctcacgg gcagtgtttt tttccatgga
      841 cttctgcttc acgatggcct cctcaccccc gccaagaagc atcttttcgc gcttctccct
      901 gagctcactt atcttctttt ccagtgacat aatcaatatt gtttattaca gtaattacct
      961 gcaaaaaaaa aagcacggca atttaagaca aaaaaatcaa atatgcacaa aattgtcaaa
     1021 agtgtgctat tttggagcgg tgtagtagac ccctgcggca atgaaagtgt aaatgatatt
     1081 gggtatccac attgcaagag cagggttcag gtcacccttg agcgagaatt gtgaggagaa
     1141 ctgcatgaag agtatatagc tgaatgtcag cacgagacca attcctatct gcattccgat
     1201 ccctcccttg atctttcgcg atgagagtga gactcctatt attgtcagta tgaagaaggc
     1261 gaatggcgag gaatagcgcc ggtgcttctc attgaggtaa agctttatct catcagatcc
     1321 ctgtgccttc agcacctgaa tatagtcgag aagttcactg tttgtcattg taaagacaaa
     1381 ctcgggtgac ctggtgaaat catcaggccc cacgttcagt actgtgtcaa tccgccgtcc
     1441 ggagctgatc cgctcaccga ggctgtcagt ttccctgata tagtaccacc aggcgcccca
     1501 tttgccggtg gtgctgtccc atctgatcat attggcagtc agctttgact cgagcatccc
     1561 ctggtcattg aacttctcca tgctgaaatt gcgaccgctc tgcgagatcc tgttatactg
     1621 ctccatatag atgtacacat tggggtagac ctgccggtgc actgatgaga cgttcaccct
     1681 cctgaagtcg gagctgcggt aatacttctc ctcgaagtca agcctggctg cattggcatg
     1741 aggcagaagg ctgttctgca acaggaaggc acagaaagca atgaaagcag ccgatataag
     1801 atagggcacc atgagcctcc tgaaactcat gccgccgttc agaatggcta tgatctcagt
     1861 gttgacggcc atccttgagg taaagaagat caccgaaata aagacaaaca tcggggcaaa
     1921 gagggtagcg aaatatggaa tgaaattgag ataatagtca aatatgatgg catgcgtcgg
     1981 cgcctgcttc tgaatgaagt cgtcaatttt ctcggaaaag tcgaatataa ccgctatgcc
     2041 cagtatgagc accagcgaaa agaggaatgt cccgaggaac tgccttatga tataccagtc
     2101 aagtatgccg ggcagcagct ttttcaggct ctttctgata ttttccgaac tgtctcttcc
     2161 ttccatgatc cgaatgtacc gtcaagtatc ctttcccgtg cctccctgac gaggctgagg
     2221 taaaacgcaa ggttgtgaat ggttgctatc tgcgcaccga gcatctcacc cgatattacc
     2281 aggtggcgga ggtatgccct ggtatatgtc ctgcttatct cagaatgtga agcggcatct
     2341 atgggcgaga agtcagttgc ccatttctga ttcctgatgt ttacaatgcc atctcgggtg
     2401 aacagcattc cgttacgtgc attgcgtgta ggcatgacac agtcgaacat atcaatgccg
     2461 cgcgctatcc cttcaagaat gtttgcaggt gttcccactc ccatgaggta gcggggcttg
     2521 tctgcaggga ggattttgtt caccacctca atcatttcgt acatcacctc ggccggttca
     2581 cctacagaca gacctccgat ggcgttgccc gccatttcgg ccgaggcaca tttctccgcg
     2641 gataatctgc gcagatcgcc gtaaacagac ccctgcacaa tagggaacag catctgctca
     2701 tgtccgtaga gcggctccgt tgaagccatt cgtgccacgc atctgtcaag ccagcggtga
     2761 gtcatctcca ttgatttcag ggcatagccg tgatcacacg gataaggagc gcattcgtcg
     2821 aaagccatga tgatgtctgc tcctatctgc cgctgtatat caataacgcc ttcaggtgta
     2881 aaaagatgct ttgaaccgtc aatgtgcgac ctgaagatgg cgccctcctc tgtcagcttc
     2941 ctgttggcag ccagtgagaa aacctggtaa ccgccgctgt cagtcagcac cgccctgtcc
     3001 caggccatga accggtgcac gcctcctgcc agcctgatta tctcagtgcc cggacgcagg
     3061 tagagatgat aggtattggc aaggatgacc ggcgcatcta tctcctgctt caggtcacgg
     3121 tgaaagaccc ccttgacgtt tgctgccgtt cccacaggca tgaaaacagg cgttggaata
     3181 tcaccatgtg ccgtgcgcaa taaacctgca cgtgccgatg ttgttccgtc tctgctctca
     3241 atagtgaaca tccgcttctc tgtgatggtc acaaaagtaa gctttctcag cctgattttt
     3301 aacagccagt tatcaaattg aagaatcctg atcatgattg ttgaaaagat tattcccact
     3361 gtcttccaat tctttttaat attgctccgg acaatattac cacacatgat catttcaact
     3421 gatacaacgc accttatact tctcgggctg ttactgacca tgtttatcat acaggcctgg
     3481 tactggcttg gttattacag aagaactgcc ttcgcgaagg tcccggagat tgctcccgga
     3541 accccgttcc cgcccctgtc agtggtaata tgcgcccgta atgaagcaga aaacctcagc
     3601 aggttcctgc ccgatgttct caaccaggac tacccttcct ttgaggtggt ggtggtaaac
     3661 gactgctccg aagacaactc ctttgatatc cttggcgggc tgatgacccg gtacccgcac
     3721 ctgaaagtct caatgatcca gaaggacccc cgtttcaaac acaccaagaa gctggctatg
     3781 ctcattggca tcaaggctgc caaaaatgac ctgctggtat ttactgacgc cgactgccgt
     3841 cctgcttcac cgcaatggct ccgtcacgtg gcttcggcag caacaggtga aaagagtgac
     3901 attgtattgg ggtacggagc ctacagcgaa gaaaaggggc tgttgaacag ctatatcagg
     3961 tatgaaacca tgtttattgc aatgcaatac ttcggaatgg ccatggcagg aaagccatac
     4021 atgggggtgg gccgcaacct ctcataccgg cgtacattct tcttcgggca gagtggtttc
     4081 ggtccacata accacataat atcaggcgac gatgacctct ttgtgaaccg cacttccaca
     4141 tcagaaaact gcagcctgat gctggct
//
LOCUS       DAOPBK010000198         4148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_17605_length_4148_cov_4.059125, whole genome shotgun sequence.
ACCESSION   DAOPBK010000198 DAOPBK010000000
VERSION     DAOPBK010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17605_length_4148_cov_4.059125"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="PLA17_09875"
     CDS             complement(<1..2213)
                     /locus_tag="PLA17_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK85760.1"
                     /translation="MKKKNNKSPFPWNSVSVILFTAAVLTAIIITYSEGQKKRLLRDA
                     VTEMEAIANVKTIQVSDWKKEHLNDATLIHENVTLVEHIDAMFIKENSIREKSRLNQL
                     LTTLIENYDFRGAVMLDAGGNPGIAVPPEEAVMGEYLRGNVTKVISNPVISLSDLHMA
                     KVIDYLHLDLLIPMRMPYEGDTVLAGVLVLRIDPEKELFPMLSTWPTPGSTSECILFR
                     FEGDSVIYLNGSHLAGHDKAVTFAAGPMELSVMNPETDANEASVYTDYTGTEVVASVR
                     KVPGTNWYIIAKKETSEILARSIREVTMLWMIMGIVYIVLAIMAWLIIKSTKARYLRE
                     RQQEELNRQAIMKHFDFVMKYGNDIILLIDSDMNIVEANERAVTTYGYPREKLIGMNV
                     SEIRTPDEVSLLSEHIRNLQETGSSYIETVNKRSDGSTFPIEVSAYTVEVEGKKFFQS
                     IGRDITDRKRTEEEMLESNKKLSTIINNLRGVVFRCNNDPDWSMQYISDGIYELAGYL
                     PNEFIDNKIRTFSSIIDPEDVEIVRNNILEALETGYLYTIEYRIITSSGSRKWVWERG
                     RGYYESDRLVALEGFISDITERKLIEEELIRAKEKAEQSDRLKTAFLHNISHEIRTPM
                     NAIVGFTTLLDSPDTTDDSRRQYIDIIYQSSNQLLSIITDIVDISNIETGLVKISRST
                     VNINQLIRKLHNQYLMRAQGQGLVLNFTTHLDDQDAQMVTDETKVIQIFSNLLNNA"
     assembly_gap    2303..2402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3308..>4148
                     /locus_tag="PLA17_09880"
     CDS             3308..>4148
                     /locus_tag="PLA17_09880"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPK85761.1"
                     /translation="MVTFTDLTQNISGEATYSWDFGNGAIPATTAGVGPHVVYYSTAG
                     SVTVTLKVTDEAGEGEETKTDYITVDGVAPFFESCPAATIIQSADAGLCSAVVDYSSL
                     VSVTGSPEPALTYSFSGATTGTGSGTGSGSSFNIGSTQVEIVAQNSCSTTDICTFTVF
                     IDTPPVAEAGGPYQTCSGSSVSLTGSSSYDPDAACGDIIASYDWDINNDGIDDLNGVS
                     LTLTHAMLTGYGLGAGVHQIRLTVTDSYGVAGTDYATITINASPVATASNNSPVCEGT
                     PLTL"
BASE COUNT          932 a          918 c         1008 g         1190 t
ORIGIN      
        1 gcattgttga gcaggttgga aaaaatctgg atcacctttg tttcatctgt caccatctgt
       61 gcatcctggt catcaaggtg tgtggtaaag ttgagcacca ggccctgacc ctgggctctc
      121 atcaggtact ggttgtggag tttcctgatc agctggttta tgttgacggt tgaccggctg
      181 atctttacca ggcccgtctc gatattggag atatcgacaa tgtcagtgat gattgagagc
      241 agctggttgc tcgactgata gattatatca atatattgcc tgcgcgagtc atccgtggtg
      301 tcaggagagt caagcagggt tgtgaatccg acgatggcat tcatcggggt acggatctcg
      361 tgcgagatgt tgtgcaggaa tgctgtcttc agcctgtcac tctgttccgc cttttccttg
      421 gccctgatga gctcctcctc tatcagcttc cgttcggtga tatctgatat gaagccttcg
      481 agggccacca gcctgtcact ttcgtaatag ccccggccgc gctcccaaac ccatttcctg
      541 cttcccgatg aggtaatgat cctgtattct atggtataca ggtatccggt ctccagagct
      601 tccaggatat tattccggac tatctccaca tcttccggat caattatcga actgaaagtt
      661 cttattttat tatcaatgaa ttcattgggc aggtaaccgg ccagctcgta gatgccgtcg
      721 ctgatgtact gcatgctcca gtcggggtcg ttgttgcagc ggaacaccac tccgcggagg
      781 ttattgatga tggtgctgag ttttttgttg ctctcgagca tctcctcctc ggtgcgttta
      841 cggtcagtta tgtcacggcc gatgctctgg aagaactttt tcccttctac ctcaacggta
      901 taggcgctga cctctatggg aaacgtgctt ccgtcgctac gcttgttaac ggtttcgata
      961 tatgatgaac ctgtctcctg gagattcctg atgtgttctg ataacagtga cacctcatcc
     1021 ggggtacgga tctcagatac attcatgccg ataagtttct cgcgcggata gccatatgtg
     1081 gtgacggccc gttcatttgc ctcgacaatg ttcatatcgc tgtcaatcag caggatgata
     1141 tcatttccgt atttcatcac gaaatcgaag tgtttcatga ttgcctgccg gttcagttct
     1201 tcctgctgtc tttcacggag gtagcgggct ttggttgatt ttataatcag ccaggccatg
     1261 atggcaagga caatgtaaac tatacccatg atcatccaga gcatggttac ttcgcgtatt
     1321 gatctggcca gtatctccga ggtttccttc ttggcgatga tgtaccagtt ggtgccgggt
     1381 actttcctga cagatgcgac cacttcagtg cctgtataat cagtgtaaac gctcgcttca
     1441 tttgcgtctg tttccggatt catgacgctc agttccatcg gaccggctgc gaaggtgaca
     1501 gctttgtcat gtcctgcaag gtgtgagccg ttgaggtaga ttactgagtc accctcaaaa
     1561 cggaacagta tacactccga ggtgctgccc ggtgtcggcc aggttgagag catcggaaac
     1621 agttcttttt ccgggtctat tctcaggacc agcacgccgg caagcaccgt gtcgccctcg
     1681 tagggcatcc tcatcgggat gagcaggtcg aggtgaaggt aatcgatcac tttcgccata
     1741 tgcaaatcag acaaggaaat gaccggattg gaaatgactt tggtgacatt gcccctgagg
     1801 tattctccca tcacagcctc ttcaggcgga acggctattc cggggttccc tcccgcgtca
     1861 agcattactg cacctctgaa gtcgtagttt tcaataaggg tggtaagcag ctggtttagt
     1921 cttgactttt cacggatgga gttttccttg atgaacatag cgtcaatatg ctctaccagc
     1981 gtgacattct catggatcag tgttgcgtca ttgaggtgct cttttttcca gtcagacacc
     2041 tgtattgttt tcacgttggc tatggcctcc atttcagtga cggcatcgcg aagcagtctt
     2101 ttcttctgac cttcggagta ggtaatgatt atggctgtaa ggactgcggc agtgaaaaga
     2161 ataacgctca cggagttcca tgggaacggt gacttattgt tcttcttttt catcgggttt
     2221 ctgaaaatgg aattcggccg gggcattaag ttaaaacaaa tgtacttaat caaccttaaa
     2281 atcaggacga ttattgtgcc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnccgcttcg gcggaacagt gaaagagacg aaatcaggct cctattttgc gttccttttt
     2461 tcctttttct ccttacgctt ctctttcaga ctctttacag gctctttctt tttgtccttc
     2521 tttgttacga gacctttcgt catgactgta gtactatgat ttaaagcacc ttataataac
     2581 aggcacatcg gtgtgccagg tgaggataaa attagtaacc gggaattgaa aatgcaagag
     2641 tgttgaataa aaagtaaaaa gttgtcaaca ggcatgaaat gttcatgaca gcttgcagat
     2701 tagtcgaaga aagggttctt tttatccaat gagaacccct tttggtcaaa tagggtttct
     2761 ttgtctgttt ctttattatt aattttaaag ttgttttctt gacggctgag catagttttt
     2821 attccgaaag gattgcccgg tgctatctgc tccggattaa ttatcttgca ctgtgtgaat
     2881 ataatatgcc cggccctgtt cacgagccgg gttctctttt ttacggctca accggaaggg
     2941 attgtcactg tgactgacgg gagcagggcg tattaaaatc acaatcagcc ggcctggtaa
     3001 caacaagaaa ggcaattggt taagggttgc gacgataaga ccggagtaat attcggggtt
     3061 ctctcttttc atgccttaca aggacttcgg aaagtttatg cagggtatga ttctgaatgg
     3121 ttgatatgga atctgtgaaa ttgcagtaaa aatgaaaaga attgtgccgg gctgcagggc
     3181 aaactgcaga aaaaggtttt taaaagtaat tgtaccgtgc ggattattcc ttgtttcatc
     3241 actgggtctg ttggcgcagc cagttgtaga tttcagtgcg gacaggacta cagtgtgtgc
     3301 cggatctatg gtaaccttta cagatctgac gcaaaatatt tcaggtgagg caacctacag
     3361 ctgggatttc gggaatggag ccataccggc aactacagcg ggcgtggggc ctcatgtagt
     3421 atattattca acagcaggat ccgtgacagt tacccttaag gttactgatg aggcagggga
     3481 aggtgaagag actaaaaccg attacatcac ggttgatggg gttgccccat tttttgagtc
     3541 ctgcccggct gccacaatca ttcagtcagc tgacgcgggt ctctgttcgg cagtggttga
     3601 ttactcctca ttagtgagcg tcacaggatc acccgagcct gctctgacct atagttttag
     3661 cggcgcaacc actggtaccg gaagcggtac aggtagcgga tcttctttta atattggtag
     3721 tactcaggtt gaaattgttg cacaaaactc ctgctcaacg acagacatct gcacttttac
     3781 ggtctttatt gacactcctc cggtagctga ggcaggagga ccctatcaaa catgctctgg
     3841 ctcttcagtt agcctgacag gcagcagttc ctatgacccc gatgctgcct gcggtgatat
     3901 tattgcttct tatgattggg atattaataa tgatggcatt gatgatctta acggggtttc
     3961 attgaccctg actcatgcaa tgcttaccgg ttacggtctc ggagcagggg tgcatcagat
     4021 ccgactgact gtaacagaca gctacggagt agccggtact gattatgcca ccatcaccat
     4081 aaatgcttcg cctgtggcga cagcttcgaa taacagcccg gtatgtgagg gtacaccgtt
     4141 aacattaa
//
LOCUS       DAOPBK010000199         4121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_17736_length_4121_cov_4.278406, whole genome shotgun sequence.
ACCESSION   DAOPBK010000199 DAOPBK010000000
VERSION     DAOPBK010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17736_length_4121_cov_4.278406"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /gene="elbB"
                     /locus_tag="PLA17_09885"
     CDS             <1..650
                     /gene="elbB"
                     /locus_tag="PLA17_09885"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HPK85762.1"
                     /translation="AAIVLAGCGVKDGAEIHEAVMTMYAIDKLGGTYYLFAPNVAQHH
                     VINHITGEEMKESRNVLTEAARIARGKISPLSDYRAEEYDTLIFPGGFGVAKNLCTYA
                     FDGPDCHVDRVVEKVIRSTYSAGKPIGALCISPVLIAKVLGDITVTTGNDRQTAAHIG
                     KLGGKHENREHGVTIDRKNLIVTAPCYMSNATISEIARDAEEVVKALIELTGKKG"
     gene            complement(723..1349)
                     /locus_tag="PLA17_09890"
     CDS             complement(723..1349)
                     /locus_tag="PLA17_09890"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HPK85763.1"
                     /translation="MRIVVIILLLGFLHAGVSGQRNDTLVDDTLMADTLLKPLHILKA
                     ATFDGQTYPHVELKEIIVTGKMPKGIRFDYRRHARLVYNVRRVYPYALIVRDEFGRIN
                     RLLETLPTERAKRDFLQSYEKDLLARYEGDMRRLTFTQGKILIKLIDRETSNTSYELI
                     RQYRGKISAIFWQGIARIFGANLKSTYDPRGDDYLIEHIVREIEAGRL"
     gene            1455..2093
                     /locus_tag="PLA17_09895"
     CDS             1455..2093
                     /locus_tag="PLA17_09895"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HPK85764.1"
                     /translation="MKHTPDIDQLDDTSLYEKVLTLPYDEIGPFVLREMTRYTRPMLR
                     IWITFAATIFLSVWFWPGVAYHPEEPRVAAGLAAGLILIPLLLVPVHEGLHLIPFLFA
                     GARDIRIGADLSQGINWVTAHRFVAGKRLFSMVASTPFILITVALMTAIFFSPPWWKW
                     VLSMALLVHTTMCAGDAALTGFMDSFGKRRVYTWDDADRKEAYFYASTETTD"
     gene            2312..3748
                     /locus_tag="PLA17_09900"
     CDS             2312..3748
                     /locus_tag="PLA17_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK85765.1"
                     /translation="MKKFPRLFWISNIIELFERWGWYAFYNGFIAIYLTSPRETGALG
                     FSNVEKGTIMGTASMILYFLPVITGAVADRVGYKKVLITSFITYVISFFMLSRFETFG
                     SIFAAFILLAVAGALFKPLISGTVARVTDRETASLGFGIFYMVINIGGFIGPFVASLL
                     YKNNWDSVFYMSIAAMTLNLLLTIFFYREPAITESGKSFIKNIGIAFRNIGITLTDWR
                     FLLFLLIIGVFWTAYNQLYYSFPVFLESWVNLDGMSLFLGLKPGTITTVTISSLASFF
                     IILFQLIISSITARFRPLNSIMTGIFILAFGLGMMFANLNPWIIVLGVLVFSIGEMAS
                     SPKIQEYLGGIAPSDRKALYMGTAFLPIALGHVGAGWISGRPFEVRADKLFLLKKAVE
                     EKGFDIPDISGSFTQTDYFNRAQELFGMDSHQLTRYLWDTYNPSRVWILFTAIAIAAS
                     VMLYLYDRLILKGREAAGNNRSAAGSQQ"
     gene            3847..>4121
                     /locus_tag="PLA17_09905"
     CDS             3847..>4121
                     /locus_tag="PLA17_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPK85766.1"
                     /translation="MKTKLIIITALLLGTMSLQAQRQMVNVPELPGYVTLKCDFHIHT
                     VFSDGNVWPTNRVSEAWRDGLDAIAITDHIEYQPHKQYIPTDHSAAW"
BASE COUNT         1080 a         1072 c          940 g         1029 t
ORIGIN      
        1 aagcagctat agtacttgcc ggatgcggcg taaaggacgg cgccgagata cacgaggccg
       61 tgatgaccat gtatgccata gacaaacttg gcggcaccta ctatctgttt gcgcccaatg
      121 tggcacaaca tcacgtaatc aaccacatta ccggagagga gatgaaggag tcacgcaacg
      181 tactcaccga ggcggcacgt atagccaggg gcaaaatcag cccactgtcc gactacaggg
      241 ccgaagagta tgacacactc atcttccccg gaggattcgg cgtggccaaa aacctctgca
      301 cctacgcctt tgacggccct gactgccatg tagacagggt ggtggaaaaa gtaatccgct
      361 caacttacag tgccggcaaa ccgatagggg cattgtgcat ctcacccgtg ctcattgcaa
      421 aggtgcttgg tgatataacg gtaacaacag ggaatgaccg gcagaccgca gcacacatag
      481 gtaaactggg aggaaaacat gaaaaccggg aacatggtgt gacaatcgac aggaaaaacc
      541 tcatagtcac ggcaccatgc tatatgagca acgctactat ctcagagatt gcccgtgatg
      601 ctgaagaagt ggtaaaggcc ctgattgaac tcaccggcaa aaaaggttga cccgggctat
      661 gattgaactg agcgggagaa acttttgaaa cagtgcatat tttcctgagc gggaaatttt
      721 tgctataacc gcccggcttc tatctccctg actatatgtt ctataagata gtcatcgccc
      781 cgggggtcat aggttgactt caggttagcc ccaaagatac gggctattcc ctgccagaag
      841 atggcggaaa tctttccgcg gtactgcctt atgagttcat acgaggtatt actggtctcc
      901 ctgtctatca gcttgatcag tatcttcccc tgggtgaatg tcagccttcg catatctccc
      961 tcataccggg caagcaaatc cttctcataa gactgcagga aatcacgctt tgcgcgctcg
     1021 gtaggaagag tctcaagaag cctgtttatc cggccgaact catccctgac aatcagagca
     1081 tacggatata cccgccgcac attgtaaaca agacgggcat gcctgcggta atcaaacctg
     1141 ataccttttg gcatctttcc ggtgacaata atctctttca gctccacatg cggataagtc
     1201 tgcccgtcaa atgtcgcagc cttaagaata tgcagtggct tcaggagagt gtcagccatg
     1261 agagtgtcat caaccagagt gtcattcctc tgaccgctga cgcccgcatg aagaaaacca
     1321 aggagcagta tgattaccac tattctcata actacctgca aatttaacca attgtcgtgc
     1381 caaaggtttc acgggaagcc aaaagtgagc attgtcatta tgtatgaaca gctatcagct
     1441 atctttggga tctcatgaaa catacaccgg atatagacca gcttgatgat acttcattgt
     1501 atgaaaaggt attgaccctt ccgtacgatg agataggacc ttttgtactc cgtgagatga
     1561 ccaggtacac acggccgatg ctccgtatct ggatcacctt tgctgcaacc atcttcctgt
     1621 cagtatggtt ctggccggga gtggcatatc atccggaaga gccgcgggtt gccgctggcc
     1681 ttgcagccgg actgatactg atcccgctgt tgctggtgcc ggtgcatgag ggattacacc
     1741 ttatcccctt cctctttgca ggagcccgcg acataagaat cggggcagat ctgagccagg
     1801 gaatcaattg ggtaacggca cacagattcg ttgcaggtaa gagactcttc tcgatggttg
     1861 catccactcc cttcatcctg attaccgtcg ctctcatgac agccatcttc ttctctccac
     1921 cctggtggaa atgggtattg tcaatggccc tgctggtaca taccaccatg tgtgccggtg
     1981 atgccgcgct gaccggattc atggacagct tcggcaaaag aagggtgtat acatgggatg
     2041 acgctgacag aaaagaagct tatttctatg cttccacaga aaccaccgat taaaaaaaat
     2101 aaccgtggaa cagtaacaat ggttcaatat atttttcaaa tgattgctta taattacctg
     2161 attatcaaaa cttatcatct ttctttaagt aaacattaca attaattgat tatgtgtctg
     2221 ttagcaaatt tttgccaatc tgttaacagt gatagataat tcattaattt aaccccgtaa
     2281 cacaacccgg atgagcagct ttatccaatc attgaaaaaa ttcccccgac tattctggat
     2341 ttcaaacatt atcgaactgt tcgaacgttg ggggtggtat gccttctaca acggcttcat
     2401 agcaatttac ctcacctctc ccagggagac tggtgccctg ggattctcaa atgtggaaaa
     2461 gggaaccata atgggcaccg cctcaatgat tctctatttt cttcccgtca tcaccggtgc
     2521 tgttgctgac agggtcgggt acaaaaaggt gctgattacc tcattcatca cctatgtcat
     2581 ctcattcttc atgctgagcc ggttcgagac attcggttcc attttcgcag ccttcatcct
     2641 cctggctgta gccggcgcac tcttcaaacc gctcatatcc gggaccgttg cccgcgtcac
     2701 tgacagggag acagcatcac tcggtttcgg aatattctat atggtgataa atatcggcgg
     2761 cttcatcggt ccgtttgtcg ccagcctgct ttacaagaac aactgggact ccgtgttcta
     2821 tatgtccata gcagccatga ccctcaacct ccttctcacc atcttcttct acagggagcc
     2881 tgccataaca gagagcggaa aaagcttcat taaaaacata ggtatagcat tcaggaatat
     2941 cgggataacc cttaccgact ggcgtttcct gcttttcctg cttatcatcg gtgtcttctg
     3001 gacagcctat aaccagctct attactcatt ccccgtcttc ctggagtcat gggtaaacct
     3061 cgacggaatg tcactcttcc tgggacttaa acccggaacc ataaccacag tcacaatctc
     3121 gagtctcgct tcattcttta ttattctgtt ccagctgatt atatcatcga taaccgccag
     3181 gttcaggccc ctgaacagta tcatgaccgg tatctttatc ctggctttcg ggctgggaat
     3241 gatgtttgca aacctcaatc cctggataat tgtccttggt gttttggttt tcagcatcgg
     3301 cgagatggcc tcttcaccca agatacagga atacctcggc ggaattgctc cgtctgacag
     3361 aaaggctctt tacatgggta ctgccttcct gccgatagcc cttggtcacg tgggagccgg
     3421 atggatctcc ggcagaccat tcgaggtaag ggctgacaag ttgttcctcc tgaaaaaggc
     3481 agtggaggaa aagggatttg atatccctga tatttccggt agcttcaccc aaaccgatta
     3541 cttcaacagg gcacaggagc tcttcggaat ggactcacat caactgacac gatatctttg
     3601 ggatacatac aacccctcgc gtgtctggat actctttacg gccatcgcca ttgccgccag
     3661 cgttatgctt tacctctatg accgcctcat cctgaaaggc cgggaagcag ccgggaacaa
     3721 caggtctgca gccggtagtc agcaataggc aggaaagggg tttggcagca tggcagacaa
     3781 cattgcagat ggcattacaa accaggatat tccccacatg ggataaaaca ttgtaaaaac
     3841 agatttatga aaacaaaact cattatcatc accgccctcc ttctgggcac catgtcatta
     3901 caggcccaga ggcaaatggt caacgttccc gaactgccgg gctatgtgac cctgaaatgc
     3961 gactttcaca tacacactgt cttctctgac ggaaacgtct ggcctacaaa ccgtgtcagc
     4021 gaagcatggc gcgacggcct ggatgccatt gccatcacag accatattga ataccagccg
     4081 cataaacaat acatacccac tgaccacagc gctgcatgga a
//
LOCUS       DAOPBK010000200         4097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_17861_length_4097_cov_4.507669, whole genome shotgun sequence.
ACCESSION   DAOPBK010000200 DAOPBK010000000
VERSION     DAOPBK010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17861_length_4097_cov_4.507669"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLA17_09910"
     CDS             <1..380
                     /locus_tag="PLA17_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPK85767.1"
                     /translation="TRIYSKARAKIADALVTGKQIIIEGGKVYFDSEWYKCNGCGCLF
                     NQPEKSVTPEKCALCGDNSIVSYDPVDDPAPDARRSFSDICVCPACGYEKRHEHSRRC
                     RDERCLVCGSRLMRKNNLPATDI"
     gene            393..716
                     /locus_tag="PLA17_09915"
     CDS             393..716
                     /locus_tag="PLA17_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPK85768.1"
                     /translation="MKVAITSTGESLESRLDQRFGRCAFFVIYDTETGGVEILPNPNK
                     DAVEGAGPASVRLVASKDVKKIISGEFGVNIKPLLDNLKIQMVAIREPERTVKSVIDM
                     LKNNQ"
     gene            1017..1292
                     /locus_tag="PLA17_09920"
     CDS             1017..1292
                     /locus_tag="PLA17_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85769.1"
                     /translation="MAQDVVRVAQMDQNVVPDMAQAAQSDQDEALVVHLEQGVMLVMV
                     LVAQQELVVAQAVQSDQDEALVARQEQGVVPVAAEAAEKGKPDSNQY"
     gene            1363..1701
                     /locus_tag="PLA17_09925"
     CDS             1363..1701
                     /locus_tag="PLA17_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPK85770.1"
                     /translation="MKKRIAIPLEGGVLCSHFGHCEQFAIVDADDKNITGETVVTPPP
                     HEPGLLPAWLAEQGITDVIAGGIGQRAISLFNRHSINVFVGAPVKSPDELAADLLNNR
                     LQTGANYCDH"
     gene            1711..2154
                     /locus_tag="PLA17_09930"
     CDS             1711..2154
                     /locus_tag="PLA17_09930"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HPK85771.1"
                     /translation="MEKKKIGIIICDRYHTCAGGKCFRSFRNREGAFDRYNGEDAEIA
                     AFTTCGGCPGGNVEYSPEEMKKNGVNVIHLATGMVVGYPPCPRIDYFTRFIPEKYGIE
                     VVVGTHPIPQKYYTTHMSLGTWKGEAMQQFIKSVLADEKMRLAYD"
     gene            2167..3039
                     /locus_tag="PLA17_09935"
     CDS             2167..3039
                     /locus_tag="PLA17_09935"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK85772.1"
                     /translation="MRIAVASGKGGTGKTFVATNLFNVLSRRGDRVTLTDCDAEAPND
                     LLFFRTEKVSVKEVTHQVPVINKDACTWCGRCSQWCSFNAIFFVPNAKVIEVIESICH
                     GCGACSYACNDEAITEKPLTLGTVTSYMTDKNNPLIEARLHVNEISTVRVINAAIKES
                     GDEGVVILDAPPGTSCPFIQTVDKADSVVLVTEPTPFGLSDLRQSVETLKEMSKEPAV
                     IINRSDIGDRDVAGYLEKEKIPLLAEIRYDPAIARAYSEGRLVTDEMPELASLFEELA
                     DKIIDRYGTGSSKR"
     gene            3014..3880
                     /locus_tag="PLA17_09940"
     CDS             3014..3880
                     /locus_tag="PLA17_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK85773.1"
                     /translation="MELAVVSGKGGTGKSSVTAAFATAGERLVVADCDVEAANLFLLF
                     NPEHDTEQVYIGGYSAVVDNALCTVCGMCESYCRFDAITRVDGKIIISERSCEGCFFC
                     SRICPVDAITMVPGDRSRMYSGSFRNGRMVYGRLAPGEENSGKLVNMVRNKAKRVAKD
                     HGMNIILLDGPPGIGCPVISTVTGVDKVIIVTEPTMSGLGDMKRVHEVARKFTGDIGV
                     IINKYDLNEAMTDSIEGWCIAQGNALLGGLPYDRRVTEAMISGKSVIEMFPGAEISII
                     LKRIWNKLTANV"
BASE COUNT         1089 a          874 c         1204 g          930 t
ORIGIN      
        1 ttacccgtat ttattcaaaa gcgagagcaa agattgccga tgcgctggta acgggaaagc
       61 agatcatcat agaaggaggc aaggtttact ttgacagtga atggtacaaa tgcaatggct
      121 gtggttgtct cttcaaccag cctgagaaga gtgttacccc tgaaaaatgc gcgttgtgcg
      181 gagacaacag tattgtcagt tatgatcctg ttgatgaccc ggcaccggat gcccgccgta
      241 gtttttctga catctgtgtc tgtcccgcct gcggttatga gaagaggcat gagcattcac
      301 ggcgctgcag ggatgagaga tgtctggttt gcggcagccg cctaatgaga aaaaacaacc
      361 ttccggcaac agatatttaa acatacggga atatgaaagt agcaataaca agtacaggag
      421 agagccttga gagcaggctc gaccagcggt ttggccgctg tgcatttttt gtcatttatg
      481 atacagagac aggtggggtg gagatcctgc caaacccgaa caaggatgcc gtggaaggtg
      541 ccggtccggc ttcggtccgg ctggtggcgt caaaggatgt gaagaagatc atctccggag
      601 agttcggagt gaatataaag ccactgcttg acaaccttaa aatccagatg gtggccatca
      661 gggagccgga aaggacagtt aagtcggtga ttgatatgtt gaaaaataac caataaatag
      721 gagaattatg cctggattag atcaaagagg tcctcatggt gagggaccga tgaccggctg
      781 gaaagccggc aagtgtacgg atttcgatgc cggcaatgcc gggatggcgg acggtgaaaa
      841 ggccggtgcc aataatctgg aattgaatgc cccggaaaca aaaggcagcg ataccggtct
      901 ggaccgtatg ccgggtatag gcaaaggtat gggcctgggc aggagacggg gaggtggccg
      961 gggcgccggc agaggtaacg ttgcaggaag aggcggtgtg tgctatggca caggctatgg
     1021 cgcaggacgt ggtgcgggtc gcacagatgg atcagaacgt ggtgccggat atggcgcagg
     1081 ccgcgcagtc ggatcaggac gaggcactgg tcgtgcactt ggagcagggg gtgatgctgg
     1141 ttatggtgct ggtcgcgcag caggagctgg tcgtggccca ggccgtgcag tcagatcagg
     1201 acgaggcact ggtcgcgcgg caggaacagg gcgtggtgcc ggtcgcggca gaggccgctg
     1261 aaaagggcaa gccggacagt aatcagtact gagagtaccc gctcaaagct tgatgacaaa
     1321 tttgtaattg attcatatac aataaataac catttcttag caatgaaaaa acgtatagca
     1381 attcccctcg aaggaggtgt gctctgttct catttcggcc attgtgaaca gtttgcaata
     1441 gttgatgccg atgacaaaaa cattaccgga gaaacagttg tcactccccc gccacatgag
     1501 cccggacttc ttcccgcctg gctggctgag cagggaatca cagatgtgat agccggagga
     1561 atagggcaga gggcaataag cctctttaac cggcactcaa taaatgtatt tgtgggagct
     1621 cctgtgaaaa gtcctgatga actggctgca gacctcctca ataacaggct tcaaaccggt
     1681 gctaattatt gtgatcatta gtatatcatc atggaaaaga agaagattgg cattatcata
     1741 tgcgacaggt accatacctg cgcaggtggc aagtgcttca ggtcgttccg caaccgtgag
     1801 ggtgcctttg acaggtacaa tggtgaggat gcggagatag cagccttcac tacctgtggc
     1861 ggctgtccgg gcggcaatgt ggagtattca cctgaggaga tgaaaaagaa cggtgtaaac
     1921 gttatccacc ttgcaacagg catggtggtt ggatacccgc catgccccag gattgattac
     1981 ttcacacgct ttatcccgga gaagtacggg atagaggtgg ttgtaggcac acatccgatt
     2041 ccgcagaagt attacacaac ccatatgagc ctgggcacct ggaagggtga ggcaatgcag
     2101 cagtttatca aatctgtgct ggctgacgaa aagatgcggc tggcatatga ttagcacaca
     2161 tgcgctatga ggatagctgt agccagcggt aagggaggca caggcaagac ctttgttgca
     2221 acaaatctgt ttaatgtgct ctcccgtcgg ggtgacaggg tgactctgac agattgtgat
     2281 gcggaggctc cgaatgacct cttgtttttc agaaccgaaa aagtatctgt gaaagaggtg
     2341 acccaccagg ttccggtcat aaacaaggat gcatgtacct ggtgtggacg gtgcagtcaa
     2401 tggtgcagtt tcaatgctat tttttttgta cccaatgcaa aagtgattga ggtgatagaa
     2461 agcatctgcc atggctgcgg ggcatgctct tatgcatgca atgatgaagc cataacagaa
     2521 aagccattga cactgggaac agtcacctct tacatgactg acaagaataa tccgctgata
     2581 gaggcacggt tgcatgtcaa cgagatctcg actgtaaggg taatcaatgc cgccataaag
     2641 gagtccggtg atgagggggt ggttatcctc gatgcgccac ccggaacatc atgccccttt
     2701 attcagacgg tagacaaagc tgactctgtg gtgctggtga ctgagcctac cccttttggc
     2761 ctcagtgatc tgcggcagag cgttgagaca ctgaaggaga tgagtaagga accggcggtg
     2821 ataataaaca gatcggatat aggtgacagg gatgttgccg gttacctgga aaaggaaaag
     2881 atacctctcc ttgccgagat caggtatgac cctgccattg cgcgggcata ctctgagggc
     2941 aggctggtga cggatgaaat gccggagctg gcttccctct tcgaagaatt ggctgacaaa
     3001 attattgaca gatatggaac tggcagtagt aagcggtaaa ggaggcacgg gcaagtcaag
     3061 tgtgacggca gcctttgcca cagccggtga aaggctggtg gtagctgact gtgatgttga
     3121 agcggcaaac ctcttcctgc tcttcaaccc cgaacatgat acggaacagg tctacatcgg
     3181 aggctattcc gctgtggtgg ataatgcgct atgtaccgta tgcggcatgt gtgagagtta
     3241 ttgccggttt gatgctatta cccgggtgga tggcaaaatt ataatttcgg agagatcatg
     3301 tgagggatgc ttcttttgtt cgcgtatctg tccggtggat gcaataacca tggtgcccgg
     3361 tgacaggagc aggatgtatt ccggcagttt cagaaacggc aggatggttt acggacgtct
     3421 tgctcccggt gaggagaact caggcaaact ggtaaacatg gtaaggaata aggcgaagag
     3481 ggtggccaaa gatcatggga tgaacataat cctgctcgat ggtcccccgg gaatagggtg
     3541 cccggtcatc tcaaccgtaa caggtgtgga taaggtcatc atcgttaccg aaccgacgat
     3601 gtcaggtctc ggggatatga aaagggttca tgaggttgcc cggaaattta ccggtgatat
     3661 tggagttatc atcaacaagt atgatctcaa cgaggccatg actgacagta ttgaaggatg
     3721 gtgcattgca cagggtaatg ctcttctcgg cggcctgcct tatgacagac gggtcacgga
     3781 ggctatgatc tctggcaaaa gcgttatcga gatgtttccc ggggcagaga tcagcataat
     3841 cctgaaaagg atttggaaca aattgacggc aaatgtgtaa ataaactgtg gctgtcaatc
     3901 ttaacatagc gcttgcgcga aacagcaaca ggcagtaatt ctccattact gcctgcaaag
     3961 caatcctcca ttactgcctg caaaaagaaa aagggtgcct tatttgtatt atgagacatc
     4021 ctcattgatt gctctattct gcctcagtct tacttcaggt agctgtcaag gaactcaaaa
     4081 aactccttct gccagat
//
LOCUS       DAOPBK010000201         4069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_18006_length_4069_cov_5.067763, whole genome shotgun sequence.
ACCESSION   DAOPBK010000201 DAOPBK010000000
VERSION     DAOPBK010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18006_length_4069_cov_5.067763"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLA17_09945"
     CDS             complement(<1..523)
                     /locus_tag="PLA17_09945"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669551.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPK85774.1"
                     /translation="MQKATKEDALLYHSRGRKGKVEVIPTKPYSTQYDLSLAYTPGVS
                     YPCLEIEKNPDDAYMYTAKGNLVAVISNGTSVLGLGDIGALAGKPVMEGKGLLFKVLA
                     DVDVFDIEIDEKDPEKLIEICAKLAPTFGGINLEDIKAPECFVVESRLREMLDIPVFH
                     DDQHGTAIISAAGL"
     gene            697..1641
                     /locus_tag="PLA17_09950"
     CDS             697..1641
                     /locus_tag="PLA17_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK85775.1"
                     /translation="MKSELQNIAIGVDIGGSHITAVAVDMTVHRIINGSRAESPVDNK
                     AEADTILTVWSDTLKQVIREIPLFNLRGIGFAMPGPFDYVRGICLIRGVQKYEKLYGV
                     NVGKAIASRLGLPCDCSVRFMNDATSFAVGEAWAGKAEGYRRMMAITLGTGFGSAFLN
                     NSIPVVEGDTVPEMGCVYHLPFGHGIADDYFSTRWFTSRYREVTGREADGVKEIAVMA
                     GRAPEVKEIFGEFGTGLGGFLAPWLKRFGAEILVIGGNISHAFNLFGPDMEKTLRNEG
                     CDTTIAISELKEDAALLGSAYLLDNNFWKAVQPALPLM"
     gene            1662..2831
                     /locus_tag="PLA17_09955"
     CDS             1662..2831
                     /locus_tag="PLA17_09955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK85776.1"
                     /translation="MNEKKIEMRLVAPVLLSFFVMSFVDLVGIGKDRVAGDLEKSDFI
                     LGLIPFAAFIWFFILSVPVGIIQARVGKKRMLNAGMTITGIGLFIPYFLDNFVAVIAG
                     FALLGIGNTIVQVSANPLLVDVVPGRRASSFLSFSQFIKAIGSMLAAPLAAVFASRFG
                     DWKLLFLVYGIISFLSVLWLGSVKIEETTATGTKASMASAFRLLGNSYILMMVLSIFL
                     VVGIDVGFNYFSGKYLASRFGFEEVAAQSGRSVYFFGRMLGTFAGALILTKLSSTKGF
                     LYSAILSIITILLIMIIPASNAAAWILVFLLGLGVANIFPLVFSLTVQKYPGRSNEIS
                     GLMMMAISGGALIPPVIGLVSDSLGVVPGMGVLLLCTVYLLIVSWIIIRKKLADI"
     gene            2889..>4069
                     /locus_tag="PLA17_09960"
     CDS             2889..>4069
                     /locus_tag="PLA17_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005680225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPK85777.1"
                     /translation="MKHLVTVALLLLLWSCARQEPVRPNIVYIMTDDHGYQAVSAYGS
                     GLNKTPNLDRIADEGIIFRNSFVCNSICAPSRAALLTGKHSHANGQIDNRVRFDSSQV
                     TFPKLLQEAGYQTALIGKWHLRSEPTGFDFWSILPGQGSYYNPDFIEMGRTCRQTGYA
                     TTIITDKAIEWLQQRDTAKPFCLLVHHKAPHRNWMPDTIDFDLYDGAVFPVPDNFFDS
                     YEGRQAAALQEMGISKDLHMSYDLKITGADGDSLTQTEKDYIWYLENRMTPEQRAAWL
                     REYDSISREFLSGRPEGDSLALYKLNRYLTDYLRTIESVDRNTGRLLDYLGSAGLLEN
                     TLVVYTSDQGFYLGEHGWFDKRFMYEESFRTPLMMRLPSSLSARGDISELVQNIDYAP
                     T"
BASE COUNT          945 a         1052 c         1072 g         1000 t
ORIGIN      
        1 gcaaccctgc ggcagagatg attgccgtgc cgtgctggtc atcatgaaac accgggatgt
       61 caagcatctc cctgagcctt gactctacca cgaagcactc aggcgccttt atgtcttcca
      121 ggtttatccc gccgaaggtg ggagccagtt ttgcacatat ctctatcagc ttctccgggt
      181 ccttctcatc aatctcgata tcaaacacat caacgtcagc taacaccttg aacaacagtc
      241 ctttgccctc cattaccggc ttcccggcaa gcgcacctat gtcacccagg cccagaacgg
      301 acgtgccgtt ggagatgacc gccaccaggt tgcctttggc agtgtacatg tacgcatcat
      361 ccgggttctt ctctatctca aggcagggat atgacacccc gggagtatag gccaggctga
      421 gatcatactg tgtagagtaa ggcttggtag gaatcacctc caccttaccc ttgcggccgc
      481 gactgtggta tagaagcgca tcctctttgg tggctttttg catgacaata agtggtttga
      541 ttatgaacgg ggcgaaataa cacgattatt attacgaatc caagatattt cttcagagtg
      601 atcctattat ttcagaatat tatgccggat tccttttgca ctctttggga aaatagatat
      661 tttcattgct ttaaaaaagg aggcgaggag gccaaaatga aatctgaact tcagaacatc
      721 gccattggcg tggatattgg cggcagtcat ataactgccg ttgccgttga catgactgtt
      781 caccgcataa taaacggctc gagggctgag agccctgttg acaacaaggc ggaagccgac
      841 actattctca ctgtctggag tgataccctg aagcaggtga tcagggagat accactcttc
      901 aacctgcggg gcatcggatt tgccatgccg ggcccgtttg actatgtcag gggcatctgc
      961 ctgataaggg gtgttcaaaa atacgaaaag ctttacgggg tcaatgtagg caaagccatt
     1021 gcctccaggc tcgggctgcc atgtgactgc agcgtgaggt tcatgaatga tgcaacctca
     1081 tttgccgtgg gcgaagcctg ggccgggaaa gctgagggtt accgcaggat gatggccata
     1141 accctgggca ccgggttcgg ctctgctttc ctgaacaata gtatccctgt tgtggaagga
     1201 gacaccgtgc cggagatggg ctgtgtctat cacctgcctt tcggccacgg aattgctgat
     1261 gattacttct ctacacggtg gttcacttcg cgctatcgtg aggtgacagg ccgcgaggct
     1321 gacggtgtaa aggagatagc cgtgatggcg ggaagagccc cggaggtaaa ggagattttc
     1381 ggtgaattcg gaaccggcct gggtggcttc ctggcaccgt ggctgaagag atttggtgct
     1441 gagatactgg tcatcggcgg aaacatatca catgccttca acctcttcgg gcctgacatg
     1501 gagaaaactc tgcggaatga gggatgtgac accacgattg caatttcgga acttaaggag
     1561 gatgctgccc tgcttggcag cgcctatctc cttgacaata atttctggaa ggcagtacag
     1621 cctgctctcc cgttaatgta agactgaata acctgaacta tatgaatgag aaaaaaattg
     1681 agatgaggct ggtggcgccg gtgctcctct ccttcttcgt tatgagcttt gttgacctgg
     1741 tgggtattgg caaggaccgg gtggcaggcg accttgaaaa gagcgatttc atactgggtc
     1801 tgattccctt cgcagctttc atctggttct ttatcctctc tgttcccgtc gggatcatac
     1861 aggctcgcgt aggaaaaaag agaatgctca atgccgggat gaccatcacg ggcatcgggc
     1921 tgttcattcc ttatttctta gataactttg tggccgtcat cgcgggcttc gccctcctgg
     1981 gtataggaaa caccattgtc caggtttcag ccaatccgct tcttgttgac gtggtgccgg
     2041 ggcgcagggc atcaagcttc ctcagcttct cccagtttat caaagccatc ggctcaatgc
     2101 ttgcagcacc gctggccgct gtcttcgcga gccggttcgg tgactggaaa ctgctgttcc
     2161 tggtctatgg aatcatctca ttcctcagtg tactgtggct tggcagcgtg aagatagaag
     2221 agaccactgc tacgggtaca aaggcatcaa tggcttcagc cttcaggctg ctcggaaact
     2281 catacatcct catgatggta ctctcgatct ttctcgtggt cggtattgac gtgggattca
     2341 actatttctc aggtaaatat cttgcctcca ggttcggctt cgaggaggtg gcggcccagt
     2401 caggccgaag cgtttacttc ttcgggcgta tgctgggaac cttcgccgga gctctcatcc
     2461 tgacaaaact ctcttccacg aaaggattcc tttacagtgc catactgtca atcatcacca
     2521 tcctgctgat tatgataata ccggcatcaa atgctgctgc ctggatactt gttttcctgc
     2581 tcgggctggg agtggcaaat atcttcccgc ttgtcttctc tctcacggtg cagaaatacc
     2641 ccgggagaag caacgaaatc tccggactta tgatgatggc catctccgga ggggctctca
     2701 taccgccggt gattggcctg gtgagcgact ccctcggcgt ggtgccggga atgggtgtgc
     2761 tgctgctctg tacggtatac ctgctgatcg tctcctggat cattatcagg aagaaactgg
     2821 ctgacatctg attcacaggc ctgaaaaggc tgtctctctg ttgaaaagca agcggtaaat
     2881 caataatcat gaaacacctt gtaaccgttg ccctgcttct cttgctctgg tcatgtgcca
     2941 ggcaggagcc tgtcagaccc aacatcgtct atatcatgac cgatgatcat gggtaccagg
     3001 cggtaagcgc atacggcagc ggacttaaca aaacaccaaa tcttgaccgt atagcggatg
     3061 aggggatcat cttccgcaac agcttcgtgt gcaattcgat ctgtgcaccc agcagggctg
     3121 cactgctgac cggaaaacac agccatgcca atggccagat tgacaacagg gtcagatttg
     3181 acagttctca ggtcaccttc cccaaacttt tgcaggaggc cggataccag actgctctca
     3241 tcgggaagtg gcacctcagg agcgaaccga cgggattcga cttctggagc atactgcccg
     3301 ggcagggaag ctattacaat cctgacttca tagagatggg acgcacctgc cggcagacag
     3361 gctatgccac caccatcatc accgataagg caatagaatg gcttcagcag agggatactg
     3421 cgaaaccctt ctgcctgctg gtgcatcaca aggcgcccca ccgcaactgg atgcctgaca
     3481 ccattgactt tgatctgtat gatggagcag tgttccccgt acctgataat ttctttgaca
     3541 gctatgaggg caggcaggct gctgccctcc aggagatggg catcagcaag gatttgcaca
     3601 tgtcatacga cctgaagatc accggcgctg acggtgactc actgacacag acagaaaaag
     3661 attacatctg gtaccttgag aaccgtatga cccctgaaca gcgggccgcc tggctcaggg
     3721 agtatgacag cataagcagg gaattcctgt caggcaggcc ggaaggagac tccctggcac
     3781 tctacaagct caacaggtat cttactgact acctccggac aatagaatca gttgaccgta
     3841 acaccggaag gctgcttgac tacctcgggt cagccggact gcttgagaac acgcttgtgg
     3901 tctatacttc cgaccagggt ttttatcttg gtgaacatgg ctggtttgac aagcgtttta
     3961 tgtatgagga gtcgttccgc acaccgctga tgatgaggct cccttcatca ctgtcggccc
     4021 gtggtgatat cagcgaactg gttcagaaca tagactatgc acccacctt
//
LOCUS       DAOPBK010000202         4005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_18349_length_4005_cov_3.676203, whole genome shotgun sequence.
ACCESSION   DAOPBK010000202 DAOPBK010000000
VERSION     DAOPBK010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18349_length_4005_cov_3.676203"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1153)
                     /locus_tag="PLA17_09965"
     CDS             complement(197..1153)
                     /locus_tag="PLA17_09965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK85778.1"
                     /translation="MDLTIVVPAYNEAESLPELAEWIDRVCTGSSIGYELIIIDDGSS
                     DGTWDAVKRISAANSRVKGVRFRRNYGKAAALQTGFEAASGNVVITMDADLQDSPDEI
                     PGLVKMIREEGYHVVSGWKKKRYDPFVKRITSKLYNYTARRFSGIKLHDFNCGLKAYA
                     GEVVKSIEVYGEMHRYIPMLAREAGFRNIGEKVVQHQARKYGTTKYGLDRFLKGYLDM
                     LTISFITRFGKSPMHFFGSLGTLMFIAGFIMAACLGIRKLSFVHQGLRAPLVTDSPYF
                     YIALAVMIIGSVFFVTGFLAELLNRRSPDRNQYLVKEKLNIS"
     gene            complement(1155..1700)
                     /locus_tag="PLA17_09970"
     CDS             complement(1155..1700)
                     /locus_tag="PLA17_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HPK85779.1"
                     /translation="MEKKRSVWKETLNYGIIYGLIMVVFSVLTYMFDLTLKTWIIWPS
                     LLLSIIVLFFLLRSYRDHYNSGYISYGKSLGAGVVITVYAAIITAIYIYVLYAFIDPG
                     LIDKSLALAEAKLAERGIPEAAIEQGLAVQAKMMTPWFTSLMSMVTNIFYGFILSLIV
                     SLFIMKKGNPLLDELDEEPQQ"
     gene            1815..1914
                     /gene="ffs"
                     /locus_tag="PLA17_09975"
     ncRNA           1815..1914
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLA17_09975"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1971..2582
                     /locus_tag="PLA17_09980"
     CDS             1971..2582
                     /locus_tag="PLA17_09980"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPK85780.1"
                     /translation="MLIKIYPENPNPAYVRRVTFVLEQGGVVIYPTDTIYAIGCDIRA
                     SKAIDRIARFKGLNPGNPDMSMIFESMSQLSEYTVIHDNNLFRLLKRNLPGPFTFIVK
                     ANNQIPAMFRHKKKTLGIRIPDNSITMAIVRDLGRPLMTTSIHDDEIIENITDPELIH
                     ENYDDFADLVVDGGIGSNEPSTVVDCTGPEPVILRQGKGILEY"
     gene            complement(2791..>4005)
                     /gene="trkA"
                     /locus_tag="PLA17_09985"
     CDS             complement(2791..>4005)
                     /gene="trkA"
                     /locus_tag="PLA17_09985"
                     /inference="COORDINATES: protein motif:HMM:NF007038.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPK85781.1"
                     /translation="TYEGSATSINILKEAIKKNTDLFIAVAHYEDTNITSAILAKKLG
                     ARKVIARIDNMEYLERNNKEFFREMGIDHLIYPELIAAKEVISLLKETGTTEFIDFAG
                     GLLSLYVQKLEKNAPVLNRTLEEVTNSLGSIKYRAVAIKRDEKTIIPHGREEFKEGDL
                     AFVISTRDGISDLMKFSGKKNIQAKSIMILGGSRIGRHIALKLQYDCQVKLIDIDMDK
                     CRDLAEALEETLVINGDGRNGDLLAEEGLRNMDAFVAVTGNSETNILACLLAKKIGVM
                     KTIAEVENMEYINLAENSGIDTIINKKIATASRIFRHTMNPNVTQVRMMTGTDAEVIE
                     YNVPENARITGGPLKLIDFPKDAIVGGGIRNYKPYIATGDTVIEANDKVVVFTLVSAF
                     EKLNRFFTGDRH"
BASE COUNT          986 a         1011 c          974 g         1034 t
ORIGIN      
        1 accagcagca ggcagtcagc atgtagttgt ctggaagtcg gcagcgggta gttagctggc
       61 aaccggcagt cagcaggtag cagcgggggg attagcatca ggcagaagct gccagcagca
      121 gccggcaatc agacgtcagc tacaaaatgc ttggtataca gcaaattagc gacttatctc
      181 agttgcagag tgactgctaa ctgatattca gtttttcctt tacaaggtac tggttgcggt
      241 caggtgaccg gcggttaagc agctcggcaa gaaacccggt gacaaagaat acagaaccga
      301 ttatcatcac tgccagcgca atgtaaaagt aaggactgtc agtaacgagc ggtgccctca
      361 ggccctgatg aacaaacgag agctttctga tgccaaggca ggcggccatg atgaacccgg
      421 caatgaacat cagcgttccc agagagccga agaagtgcat ggggctcttg ccaaacctcg
      481 ttataaagct tattgtcagc atgtcaagat aacccttcag gaatctgtca agaccgtact
      541 tggtggttcc atacttacgg gcctggtgct gtaccacctt ctcgcctata ttcctgaagc
      601 ctgcctcacg cgccagcatc gggatatacc tgtgcatctc accgtaaacc tcaatactct
      661 taaccacctc accagcataa gccttcagcc cgcagttgaa gtcgtgcagc tttattccgg
      721 agaatctcct tgcagtgtag ttgtatagtt tggaagtgat tcgcttgaca aaagggtcat
      781 acctcttctt tttccatccg gagaccacat ggtaaccctc ttcgcggatc atcttcacca
      841 atccgggaat ctcatcagga ctatcctgaa gatcagcatc catggtaatc accacgttgc
      901 ctgaggcagc ctcaaagccg gtctggaggg ctgctgcctt gccgtaattg cggcggaaac
      961 ggactccctt tacccggctg ttggcggctg agatgcgctt aaccgcatcc caggtcccgt
     1021 cagagctgcc atcgtcaata atgattaatt cgtaaccgat tgatgacccg gtacaaaccc
     1081 tgtcaatcca ctcagccagt tccggcagtg actccgcttc gttataagcg ggaactacaa
     1141 tggtcagatc catactactg ctgtggttct tcatccagct cgtcgaggag tggattgcct
     1201 ttcttcataa taaacagtga cacaatcagc gacagaatga aaccgtaaaa gatattggtg
     1261 accatgctca tgagagaggt gaaccagggt gtcatcattt ttgcctgcac cgccaatccc
     1321 tgttctatgg ctgcttcggg gatacctctc tcagccaact tcgcctcagc cagggccagc
     1381 gacttgtcaa tcaggcccgg atcaataaag gcatagagca catatatata tattgctgtg
     1441 atgatagctg cataaacagt gattacaact ccagctccca atgacttgcc atatgagatg
     1501 tagccgctgt tgtagtggtc acggtacgag cgaagaagga aaaagaggac aatgatgctc
     1561 agaagcaggc ttggccagat aatccacgtc ttcagggtta aatcaaacat gtaggttaag
     1621 accgagaaga ctaccatgat gagaccgtag atgataccgt agtttagggt ttctttccag
     1681 actgaccttt tcttttccat tttattgcgt ttttcagatt acagaagcaa atatacttta
     1741 tttgcgtgaa tgggaaatca ccggattgtg gatcaacatt tggtgcttgc cgcagaaagt
     1801 actatttttg tgcggggtaa gtctcatacg accagctccc tttgaactcc cccagggtcg
     1861 gaaggcagca agggtagaag gttgtagcgg tgcgatatga gcagcttacc cttctttttt
     1921 ttatccctct catcagaagt ttatatattt gccgtcactt taccaaagcg atgctgataa
     1981 agatataccc ggaaaatccg aaccctgctt atgtgcgaag ggtgacgttc gtcctggaac
     2041 agggcggcgt ggtcatttac cctaccgaca ccatttatgc cattggatgt gacatcaggg
     2101 catcaaaggc aattgacagg attgcaagat tcaagggact taatccggga aatcccgaca
     2161 tgtcaatgat ttttgagtcg atgagccagc tctctgaata cacagtcatt catgacaata
     2221 accttttcag actgctcaag cgcaacctgc cgggcccgtt taccttcatc gtgaaggcaa
     2281 acaaccagat acctgcaatg ttcagacaca aaaagaagac gctgggcatc cgcatacctg
     2341 acaacagcat caccatggca atagtgcgtg acctgggacg gcccctgatg accacatcaa
     2401 tacatgatga tgagataata gagaatatta ctgaccctga gttgattcac gaaaattatg
     2461 atgactttgc cgacctggtt gttgatggcg gcattggaag caatgaaccc tcaacagtag
     2521 tcgattgcac aggtcctgag cctgttatac tgagacaggg gaagggtata ctcgaatact
     2581 gaatcaccgg cagtggaggt attgtaactg ccgggctgtt tctggcacgg atcggtaacc
     2641 tcaagaactt cgcatgtttc ggggtaattg taactgtcgg gctgaatgtg tcgcaagccg
     2701 gttacctcaa tgacttcgca tatttctgga taattgtaac tgccgggctg tttctggcac
     2761 aaaccggttg ccaacaaccc gccactggtt ctagtgccgg tctcctgtga agaacctgtt
     2821 cagcttttcg aaagctgaca ccagtgtaaa gaccaccacc ttgtcattgg cctctatcac
     2881 ggtatcaccc gttgcaatat atggtttata gttgcgtatc cctccgccaa cgatggcatc
     2941 cttggggaaa tcgatcagct tgagcgggcc cccggtgatt cgtgcattct caggcacatt
     3001 gtattctatc acttcggcat ctgtccctgt catcatcctt acctgtgtga cgttggggtt
     3061 catggtgtgc ctgaagatac ggctggcagt ggcaatcttc ttgttgatga tggtgtcaat
     3121 gcctgagttc tcggccaggt tgatatactc catattctcc acctcggcga tggtcttcat
     3181 tacaccaatt tttttagcca gcaggcaggc aaggatattt gtctcggaat taccggtcac
     3241 ggccacgaag gcatccatat tccggaggcc ctcttcggct aacaggtcac cgttcctgcc
     3301 gtcaccattg atgacgaggg tctcctcgag cgcctcggcg aggtcacggc atttatccat
     3361 gtctatgtca atcagcttca cctggcagtc atactgcagc ttcagtgcaa tatgcctgcc
     3421 tatccggctg ccgccgagga tcatgatgct ctttgcctga atgttcttct tcccggaaaa
     3481 cttcatcagg tcagagatac cgtcacgggt cgagatcacg aatgccaggt caccctcctt
     3541 aaactcctcc cttccgtggg ggatgatagt cttctcatct ctcttgattg ccacggcacg
     3601 gtactttatt gatcccaggg agttggtcac ctcctcaagg gtgcggttaa gcaccggtgc
     3661 gttcttctcc agcttctgaa catagagtga gagcagacca ccggcaaagt caatgaattc
     3721 agtggtacct gtctctttca aaaggctgat aacctccttg gcggcgatga gctcagggta
     3781 tatcaggtgg tcaattccca tctcacggaa gaactccttg ttgttccgct caaggtattc
     3841 catattgtct atgcgggcta tcaccttacg cgcgccaagc ttcttggcaa ggatggcgga
     3901 agtaatgttg gtgtcttcgt aatgggcaac cgcaataaag agatcggtgt tcttcttgat
     3961 tgcctccttg agtatgttta tggatgtggc tgatccctca taggt
//
LOCUS       DAOPBK010000203         3984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_18465_length_3984_cov_5.246628, whole genome shotgun sequence.
ACCESSION   DAOPBK010000203 DAOPBK010000000
VERSION     DAOPBK010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18465_length_3984_cov_5.246628"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLA17_09990"
     CDS             complement(<1..893)
                     /locus_tag="PLA17_09990"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK85782.1"
                     /translation="MRKVRYIICMILMVHAVSLAGQTKSSLRELFVSAEGDLLFEDYA
                     EALPKYMSLLQIYPENFNLYYRIGQCYLNTPGEKDKAIPWLETAAGHINPNYRRGKLH
                     ETGAPYDALYFLANAYRIANDFDRALETYDLFLKDVDTEKYDTALVRFQMQTCHNARE
                     MMQKPVFITDRNLGPTINERFSEFNPVISDDEKTLLFTRELQFYDAIFWSRKIDGQWT
                     EPVNLTPQLGIDQDYYTSSLSPDGRTLLMYRTDTYDGNIYLSRLEGNTWSKVEKLNSN
                     VNTRYWESHATMSRDGEKLFF"
     gene            complement(971..1843)
                     /locus_tag="PLA17_09995"
     CDS             complement(971..1843)
                     /locus_tag="PLA17_09995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK85783.1"
                     /translation="MELSAVIVSYNVRELLRGALQSLISAAQATEHEIIVVDNNSSDG
                     SAGMVSQEFPSVKLIVSESNEGFASACNRGILASSGEYILILNPDTVTRPDAITRALD
                     FIKTDPDAGAVGAHMTDKNGKFLPESKRGFPSPLTSLFRFTGLSRLFPRSAFFNAYYL
                     GDKPENETCRADILTGAFMLIRRKALDEAGLFDTSFFMYGEDIDLSWRIRKAGFSNYY
                     LHDVHITHFKGSSSGQEMETSVRHFWNAMIIFSEKHLRKGWHVPVRAAVSLLKWLSLT
                     RLRVAKMVKSENPC"
     gene            complement(1848..3062)
                     /locus_tag="PLA17_10000"
     CDS             complement(1848..3062)
                     /locus_tag="PLA17_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPK85784.1"
                     /translation="MADTFDRFFSDATHLMKKSAIREILKLTQRPEMISFAGGLPSPD
                     SFPIDDIRRVTSEVLEQDGKSALQYGTTEGDNKLRALLAERHRKDGLEIGPENILITT
                     GSQQALDLCGKIFVNRGDVVLCGLPSYLGGLNAFANYGALLKGIPLDDHGMHPELLEK
                     TITDLKKEGKIIKFIYMIPDFQNPTGVTMPASRRLEIIRIAERHDLLIVEDSPYRDVR
                     FAGTPEPLMSSLDTHGRVITLNTFSKILAPGFRLAWVTGHREIIDKMVTAKQSTDLCT
                     PPFVQKIVARYIEQGLLDANLKKTIALYHRRRDFMLKCFRELMPEGVKWTEPDGGLFL
                     FLTLPTAMDAARLLDKAICKNVAFVCGSVFYCNNEGHNTMRLNFSYADEEDTLEGIRR
                     LAEVIREEMDTP"
     gene            complement(3412..>3984)
                     /gene="recR"
                     /locus_tag="PLA17_10005"
     CDS             complement(3412..>3984)
                     /gene="recR"
                     /locus_tag="PLA17_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPK85785.1"
                     /translation="ASLPGIGRKTAFRLVMHLLRRDSGEVRRFGETVIRLRDDVCYCS
                     VCHGISDTPLCQICSDQSRDTSVICVVENVQDVMAVENTRQFRGLYHVLGGIISPVDG
                     IGPSDLTINSLEERVRSGSVREVILALSTTMEGDTTNFYIYKRLSGYDVRITTLARGV
                     AIGDVLEYTDEITLGQSIVNRCLFSLPSQG"
BASE COUNT          905 a         1005 c         1038 g         1036 t
ORIGIN      
        1 ctgaaaaaga gcttctcccc gtccctgctc atggtggcgt gtgactccca gtacctggta
       61 ttaacattgc tgttgagttt ttcaaccttg ctccatgtat ttccctccag ccggctcagg
      121 tagatatttc cgtcataggt gtcagtacgg tacatgagga gggttcgccc gtcaggagaa
      181 agggatgagg tgtaataatc ctggtcaatc cccagttggg gagtcagatt gaccggctct
      241 gtccattggc cgtcaatttt ccgtgaccag aatatagcat cgtagaactg caattcacgt
      301 gtgaaaagca gtgttttttc atcgtctgaa attaccgggt tgaattccga gaagcgctca
      361 ttgattgttg gcccgaggtt acggtcagtg atgaataccg gtttttgcat catctcacgg
      421 gcgttgtgac aggtctgcat ctgaaatctt accagggcag tgtcgtactt ttcggtatca
      481 acatccttca gaaagagatc atatgtctcc agtgccctgt caaagtcatt ggcgatgcgg
      541 tatgcgtttg ccaggaaata gagggcgtca tacggtgccc cggtctcgtg cagctttcct
      601 ctccggtagt tcggatttat atgccctgcg gcagtctcaa gccaggggat agccttatcc
      661 ttttctcccg gagtattgag gtaacactgg ccaatacggt aatagagatt aaaattctcg
      721 ggataaatct gaagcagact catgtatttg ggcagggctt ctgcgtaatc ttcaaagagg
      781 aggtccccct cggcagatac gaaaagttcg cgcaagctgc tttttgtctg accggcgagg
      841 gatacagcat gaaccataag gatcatacaa attatgtatc taacctttct catcaggttg
      901 atcaaatatt ctttatgtaa ctaatataac tcaaattctg tcataagatt atagtagagc
      961 agctcctttt ttaacaggga ttttcacttt ttaccatttt ggccacccga aggcgggtca
     1021 gtgagagcca tttgagaagg cttacggccg ccctgacagg cacgtgccac ccctttctca
     1081 gatgcttctc cgagaagatt atcatagcgt tccagaaatg tctgacagag gtttccatct
     1141 cctgacctga actacttccc ttaaaatgcg tgatgtggac atcatgaagg taataattgc
     1201 tgaaacctgc ctttcttatg cgccagctga ggtcaatgtc ttcaccgtac ataaagaagg
     1261 aggtgtcaaa caggcctgct tcatcaagcg ctttcctgcg gatgagcatg aaggctccgg
     1321 tgaggatgtc tgcgcggcag gtttcgttct caggcttatc tcccaggtag tatgcattga
     1381 agaaggcgga gcgcgggaag agcctgctca ggccggtaaa cctgaaaagt gaggtaaggg
     1441 gtgacgggaa gcctcttttc gattcgggca ggaatttgcc gtttttatcg gtcatgtggg
     1501 caccaactgc tcctgcatcc ggatctgttt taatgaaatc cagggccctg gtgatggcat
     1561 cgggccgggt gacagtgtca ggattcagta taaggatgta ctctccggag gaggccagta
     1621 tgcccctgtt gcatgctgaa gcgaaacctt cgttgctctc tgaaacgatc agcttcaccg
     1681 aagggaattc ctggctgacc attcctgcag agccgtctga ggagttgttg tcaacaacta
     1741 tgatttcgtg ctctgttgcc tgtgcggcag atatgagtga ttgcagtgca ccgcggagca
     1801 gttccctgac attgtaggaa acaattactg cagagagttc catcggatca gggggtgtcc
     1861 atttcctccc ttatcacttc cgccagccgg cggatacctt caagggtatc ctcttcatct
     1921 gcgtatgaga aattaagccg catggtgtta tggccttcat tgttgcagta aaatactgat
     1981 ccgcagacaa aagccacatt cttgcaaatg gctttgtcaa gcagacgtgc ggcatccatg
     2041 gcagtaggaa gggtcaaaaa gaggaaaagt ccgccgtcag gctcagtcca tttcacacct
     2101 tcaggcatca gctcacggaa gcatttcaac atgaaatcac gtctccggtg gtacagagct
     2161 attgtcttct tcaggttcgc atcaagcagc ccttgctcta tgtatctggc tactatcttc
     2221 tgaacaaagg gaggggtgca gaggtcggtt gactgttttg ccgtcaccat cttgtcaatg
     2281 atctccctgt ggccagtcac ccaggccagc ctgaatcccg gtgcaagtat cttggagaag
     2341 gtgttgaggg ttattaccct gccatgcgtg tcgagggaag acatcagcgg ttcaggagtc
     2401 cctgcgaaac gcacatcgcg ataaggactg tcctctacaa tcaaaagatc atgcctttcg
     2461 gctatgcgga tgatctcaag gcggcgtgac gcgggcatag tgacgcctgt aggattctga
     2521 aaatcaggaa tcatatagat gaactttatg atctttcctt cctttttcag atcggttatg
     2581 gttttttcaa ggagttcagg atgcattcca tggtcatcaa gtggaatccc cttcagcagt
     2641 gccccgtaat ttgcgaaggc gttgaggccg cccaggtatg aaggcagtcc gcacagcacc
     2701 acatcaccgc ggttgacaaa gatctttccg cacaggtcaa gcgcctgctg tgagccggtg
     2761 gtgatcagga tattctccgg tccgatctca agcccgtctt ttcggtgccg ttcggcaagg
     2821 agagcccgca gtttgttgtc accctcggtg gtgccgtact gaagggctga tttgccatcc
     2881 tgttcaagta cctctgaggt cactctccgg atgtcatcta tgggaaaaga gtcaggtgag
     2941 gggagacctc ctgcaaagga gatcatctcc gggcgctgcg tcagcttgag tatctccctg
     3001 attgccgatt ttttcatcag atgggttgca tctgaaaaga atctgtcaaa tgtgtcagcc
     3061 ataccaatat tgatttgtat caaattaagc aagattttca gaatatacaa atgaccaaag
     3121 ccggctggct taaaaagata ttgccgcgca tgtctgtcag ctttggctgc cccgtgagcc
     3181 aacctttcag ccctgtatcc ctatcggcca ggcccctcgg ggtgcatctc tgtcagcttt
     3241 ggctgcctca ggtgcctgcc tgtcagtgcc ggctggcccg ggagtatagc tgtcagtgcc
     3301 ggctggcccg ggagtatagc tgtcagtgcc ggctggcccg ggaacttagt tgtcagcccg
     3361 gtatgtccag gggccaggtt gtcagccctg actgcccctt gagtcaacct gtcagccctg
     3421 tgacggaagt gagaaaaggc agcggttcac tatcgattgc cccagggtga tctcatcggt
     3481 atattcgaga acatctccaa tggccacgcc gcgcgccagt gttgtaatgc gcacgtcata
     3541 gcccgacagc cgtttgtaga tatagaaatt tgtggtgtct ccctccatgg ttgtgctcag
     3601 tgcaagtatc acctccctga cgctgccgct tcgcaccctt tcttccaggc tgttgatggt
     3661 caggtcagaa ggaccgatgc cgtcaaccgg ggagatgatg ccgccaagta catggtacaa
     3721 tcccctgaac tgcctggtat tctccaccgc catcacatcc tgtacgttct ctaccacgca
     3781 tatgacagag gtgtccctcg actgatcact gcatatctgg cacagcgggg tatcactgat
     3841 accgtgacaa acactgcagt aacacacatc atccctgagc ctgatgactg tttcgccgaa
     3901 acggcggact tccccgctgt cacgccttaa caggtgcatc acaagcctga aggcggtctt
     3961 gcggcctatg ccggggaggg aggc
//
LOCUS       DAOPBK010000204         3980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_18487_length_3980_cov_4.795159, whole genome shotgun sequence.
ACCESSION   DAOPBK010000204 DAOPBK010000000
VERSION     DAOPBK010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18487_length_3980_cov_4.795159"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /gene="plsY"
                     /locus_tag="PLA17_10010"
     CDS             complement(<1..504)
                     /gene="plsY"
                     /locus_tag="PLA17_10010"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPK85786.1"
                     /translation="MIISLSVIAVVLAYLAGSIPSSVWAGKLFHGIDIREHGSGNAGA
                     TNTIRVLGWKNGIPVLIFDLFKGWLAAMLPKLLAAAPENSETMMVLMIVCGMAAILGH
                     VFPLFAGFRGGKGVATTFGVLLALHPLLTLTCAGIFLLVLFISGYVSLSSMTAVTMFP
                     ILLVTVFR"
     gene            complement(515..2131)
                     /gene="pckA"
                     /locus_tag="PLA17_10015"
     CDS             complement(515..2131)
                     /gene="pckA"
                     /locus_tag="PLA17_10015"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPK85787.1"
                     /translation="MSEEILKQYGITEVQGIVYNPSYDRLYEEELSPGLKGFEKGTLT
                     TTGAVAVDTGIFTGRSPKDKYIVRDNTTENTVWWKSESTSVSDNKALAYEAWDHCKKI
                     AVRQLSGTKLYVMDCFSGANRDTRLCVRFIMEVAWQAHFVKNMFIRPTEDELKDFKPD
                     FVVLMASKAVNPEYQKFGMNSENFVLFNLTERMALVGGTWYGGEMKKGIFSVMNYYLP
                     LKGIASMHCSANVGKKNDVAIFFGLSGTGKTTLSTDPDRALIGDDEHGWDDDGIFNFE
                     GGCYAKTINLSEENEPDIYRAIRRDALLENVVVDEKGVIDFASAAKTENTRVSYPIYH
                     IDNIVRPVSRAGHASTVIFLTADAFGVLPPVSRLTEEQTKYYFLSGYTAKVAGTERGI
                     KEPVPSFSACFGAAFLLLDPIIYARELTRKMRMHNADAWLVNTGWMGGPYGTGKRIDL
                     PSTRIIIDTILNKTINDVPYTTIPVFNLSIPTSLPGVPSDLLDPGKKWPDYEAWLKAA
                     RELGEKFVKNFSKFISNAETAKLVSSGPKP"
     gene            2275..3186
                     /locus_tag="PLA17_10020"
     CDS             2275..3186
                     /locus_tag="PLA17_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85788.1"
                     /translation="MRTVFLLSALLLTISINAQSIRVRSVKKVSPGEGGYYLLTGVVP
                     GSGLLMVSGSGYNGLSLLDARRGSVQVITNDNGAGYEPAVTADGRKVVYRSDRFIDNV
                     KYSSVSIFDIATGEKELLLEKGRDVLPPAVAGNNVLIRSDKVTKVEQAGPALKSGNNE
                     TFLLTEDLMPVICSGSERKALMPNGDGFYIWVSLSPDGSKILYNYRGQGAFICDTDGT
                     ILYDLGRINAPRWFNDRIVIGMDDKDDGHRITESELVYYSLGDKKRVTLTSTPDRSEM
                     FPFAAGNRQIAFCTDNGEIYIMKVRIR"
     gene            3183..>3980
                     /locus_tag="PLA17_10025"
     CDS             3183..>3980
                     /locus_tag="PLA17_10025"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPK85789.1"
                     /translation="MNSRKYCWLTALLLCLPLFMYGQRTDFSGLKIFINPGHGGHDSD
                     DRHMIATDFWESEGNLSKGLFLRDLLQARGATVHMSRITNYTSDDLKLSVIAEMANQA
                     NVDFFLSIHSNGFDGTRNQPLVLFRGYDNSPVFPAAKTMAQILWEKLYEKSNCWTHSF
                     QWVKGDWTFYPEWGDKVGLGVLRTLAMPGVLSEGSFHDYVPEGWRLRNENHLHHEAWA
                     MLRALQQHFNVAVEPTGIIAGTVRDELTAPPYYFGPGTRDQHIPINGA"
BASE COUNT          990 a         1001 c         1036 g          953 t
ORIGIN      
        1 cctgaacact gtaaccagca gtatgggaaa catagtcacg gcagtcattg agcttagcga
       61 gacgtaaccg ctgataaaga gcaccagcag aaagatgcct gcgcatgtca gggtcagcag
      121 ggggtgcagt gcaagcagca caccgaatgt tgttgcaact cccttgcctc cgcgaaaacc
      181 tgcaaagaga gggaacacgt gccccagtat ggctgccatg ccgcatacta tcatcagtac
      241 catcattgtc tcggagtttt ccggggcagc agcgaggagt tttggcagca tggcggccag
      301 ccatcccttg aagaggtcga agatgagaac gggtatgcca ttcttccatc cgagcaccct
      361 tatggtgttg gtggctccgg cattgccgct gccatgctcc cggatatcaa tgccgtgaaa
      421 gagcttacct gcccagacgg aactggggat tgagcctgcc aggtaagcca acacgactgc
      481 aataactgat aacgaaatga tcattgacgt tctcttacgg cttaggcccc gaagatacga
      541 gttttgccgt ttcggcgttc gagatgaact tgctgaagtt ctttacgaat ttctcaccga
      601 gttccctcgc tgccttcagc catgcctcat aatcaggcca ttttttgccc ggatcgagca
      661 ggtcagaggg cacgccgggc aatgatgtgg gaatgctcag gttaaagacc gggatagtag
      721 tgtagggcac gtcgttgatg gttttattca atatggtgtc aatgattatg cgggttgaag
      781 ggaggtctat ccgcttgcct gttccgtatg gtcctcccat ccatccggta ttgaccagcc
      841 atgcatcagc gttgtgcatc ctcatcttcc gggtcagttc acgtgcgtat atgatcgggt
      901 caagcagcag aaacgccgct ccgaagcatg ccgagaagga gggtacgggc tccttgattc
      961 ctctttcggt gcctgccacc ttcgcggtgt agccgctgag gaaataatac tttgtctgct
     1021 cctcagtcag cctggagacc ggcggcaaca ccccgaaggc atctgccgtg aggaaaatga
     1081 cggtgctcgc atgacccgcc ctggagacag gcctgacaat attgtcaata tggtatatgg
     1141 ggtaggagac ccgcgtattt tctgtcttcg ctgcactggc gaagtcaatc acgcccttct
     1201 catccacaac cacgttctca agcagtgcat cgcgcctgat ggcgcgatat atgtcaggct
     1261 cattctcctc gctcaggttg atggtcttgg catagcaccc cccctcaaag ttgaagatgc
     1321 catcatcatc ccagccatgc tcatcatcac cgataagcgc cctgtcggga tcggttgaga
     1381 gggttgtctt gcctgttccg gataagccga agaagatggc aacatcgttt ttcttaccga
     1441 cgttggcaga acagtgcatt gaggcaatac ctttcagcgg gaggtagtag ttcattacag
     1501 agaagatacc cttcttcatt tcgccgccgt accaggtgcc gcctaccagc gccatccttt
     1561 cggtcaggtt gaacagtaca aagttttctg agttcattcc aaacttctga tattcgggat
     1621 tgaccgcctt tgaagccatc agcaccacaa aatcaggctt gaaatctttc agctcatcct
     1681 cagtgggacg aatgaacatg ttcttgacaa agtgagcctg ccatgccacc tccattataa
     1741 aacgcacgca gagcctcgta tccctgttgg cgccactgaa gcagtccatg acgtacagct
     1801 tcgtgcctga aagctgtcgc acggcaatct ttttacagtg gtcccatgct tcatacgcaa
     1861 gtgccttgtt atcagagaca gaagtgctct cagacttcca ccagacggta ttttcagtag
     1921 tattgtcacg cacaatgtac ttgtcctttg gcgaacgacc tgtaaatatt ccggtatcaa
     1981 cagccaccgc accggtggtt gtcagtgtgc ccttctcgaa tcccttcagg ccgggtgaga
     2041 gctcttcttc gtaaagacgg tcataggaag gattatagac tatcccttgt acttcagtaa
     2101 taccgtattg tttcaggatt tcttctgaca tcatgattac ggggtttttg atgtccatca
     2161 aagttaacaa tataagcaac taaatacact gataaaaatc aagaggccgg ctttaaagaa
     2221 aaagtgacaa ccaaacactc tattgactat ctttagaggg caataccaat cagtatgaga
     2281 actgtttttc ttttatcggc attactcctg acaatcagca taaatgcgca gagtatcagg
     2341 gtcaggagcg taaagaaggt atcacccgga gagggcgggt attacctgtt gaccggtgtt
     2401 gtaccggggt cagggctcct catggtgtca ggaagcggat acaacgggct cagtctgctt
     2461 gatgccagga gaggcagcgt gcaggtgata accaatgata acggcgccgg ttatgaacct
     2521 gcggtaaccg ctgacggccg gaaggttgtg tacaggagtg accgtttcat tgataacgtc
     2581 aaatactcgt ctgtaagcat ttttgacatc gcaacgggcg agaaagaact gctcctggaa
     2641 aaagggaggg atgtccttcc tcccgcagtg gcgggaaaca atgtccttat cagatcggat
     2701 aaggtaacga aggtggaaca ggcaggcccc gcactgaaat caggcaacaa cgaaaccttc
     2761 cttctgacgg aggatctgat gccggtgatc tgtagcggca gcgaaagaaa agcccttatg
     2821 cctaacggcg acggattcta tatttgggta tcactctcgc ctgacggcag caagatccta
     2881 tataactacc gggggcaggg cgcattcatc tgtgatactg acggcactat tctgtatgat
     2941 ctgggcagga tcaatgctcc caggtggttc aatgacagga ttgtgatagg aatggatgac
     3001 aaggatgacg ggcacaggat aaccgaatca gaattggttt actattccct tggagacaag
     3061 aagcgggtaa cactcacctc aacacccgac aggtcagaga tgttcccgtt cgcggccggc
     3121 aacagacaga ttgccttctg cactgacaac ggggagattt acattatgaa agtacgcatc
     3181 agatgaactc acggaaatat tgctggctga cggctctgct gctatgcctt ccgctgttta
     3241 tgtatggcca gagaacggac ttcagcggct tgaagatttt tattaatccc ggtcatggag
     3301 ggcatgacag cgatgacagg cacatgattg cgaccgactt ctgggagtcg gaggggaacc
     3361 tttccaaggg gctcttcctg cgcgatcttc tgcaagcccg gggagccact gtccatatgt
     3421 cgagaataac aaattacacc tctgatgacc ttaaactctc agtcatagca gagatggcca
     3481 accaggcgaa tgttgatttt ttcctttcga ttcacagcaa cggttttgac ggtacccgga
     3541 accagccgct ggtactcttc aggggctatg acaactcacc tgttttcccg gctgcaaaaa
     3601 caatggcaca gatcctttgg gagaaactct atgagaagag caactgctgg actcattcat
     3661 ttcaatgggt taagggcgac tggacctttt atcctgaatg gggagacaag gtgggtctgg
     3721 gtgtgctcag aacccttgcc atgcccgggg tgctgtcaga aggatcattc catgattatg
     3781 tacctgaggg gtggaggctc cggaatgaaa accatctcca ccatgaagca tgggcaatgc
     3841 tcagggcgct gcaacagcat ttcaatgttg ccgttgaacc gacaggaatc atcgccggca
     3901 cggtcaggga tgagctcacc gctccgccat actactttgg tccgggcaca agagatcagc
     3961 acattccgat aaacggagca
//
LOCUS       DAOPBK010000205         3920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_18840_length_3920_cov_4.064166, whole genome shotgun sequence.
ACCESSION   DAOPBK010000205 DAOPBK010000000
VERSION     DAOPBK010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18840_length_3920_cov_4.064166"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLA17_10030"
     CDS             <1..306
                     /locus_tag="PLA17_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPK85790.1"
                     /translation="NRALFRKIDQFEKDVISGKINIRQDSVRSAISQLKKDDDTGPIV
                     LIKAYKTAKYKNFVDIIDEMSIVGIARYIFTDIDWIEENLVVDALNRAAAAPSGSAQ"
     gene            319..1098
                     /locus_tag="PLA17_10035"
     CDS             319..1098
                     /locus_tag="PLA17_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPK85791.1"
                     /translation="MANKRKRYPSFDDIVFEGRNKEYGAYELRRKYSRTMTVSIIVGV
                     IVVFIAAYVPYLKARSIAHVKERDATEVIAEMANDINQEAAAPPPPPPPPPPTEQQTV
                     VKYVAPVIVDSIKPEEASQFMAMDDVAETVVDEDVGLEVIEQVQEEIEEEAPQEVFVV
                     VEEMPSFPGGDAELFNFIYSNINYPEIAKENNIQGRVTLRFCVTYKGTVDQVSVVRGV
                     DPSLDEEAIRVIKMLPLWKPGKQGGKPVNVWYSVPIKFELK"
     gene            complement(1177..2019)
                     /locus_tag="PLA17_10040"
     CDS             complement(1177..2019)
                     /locus_tag="PLA17_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85792.1"
                     /translation="MKRTLFISVLLIVSFQTIQGQGLFPELRGYKISDDYPVYTPDDL
                     WNYINGAADAYLQLGFIDLNIREYVKGKNIIKAEIYRFGDDARAFGMYSMERSPGYKF
                     INTGVQGYNEEGVVHFYKDCFYVKIMSHSLSAKVNSSMLDLATLISSRMGGSGEFPAL
                     LRLFPSEGLLENQETYLLESVLGHEFLRGAFRASYEVEGDRFDIYLFSREDPAEVAAM
                     AASLAGDAWAADDEVFRYAFKDGFNGLLYMARQGGKLILISGLPLERSSLAERYITLM
                     LGSH"
     gene            complement(2032..3213)
                     /locus_tag="PLA17_10045"
     CDS             complement(2032..3213)
                     /locus_tag="PLA17_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK85793.1"
                     /translation="MKRRNFFRSGLAGAAGITLMGNRALAGNSKRDEGEIVYRTLGRT
                     GLRLPVVSFGVMRADNSALVKAAYEGGMKLFDTAHGYMNGRNEEMLGNFFSTVPRESF
                     VLATKVKPAGVDRTGLPSKDTTAERFLEMFDISLSRLKVDYVDILYMHSADTAEMVRH
                     KETVKAMLDLKKQGRVRFIGISTHKNEPLVINTMVEEGIWDVVLTSYNYRQTYIDEMN
                     SAIEKAAAAGMGVVAMKTLAGGWLDRERTKPVNVQAALRWVLSNPNVHTTIPGITAFD
                     QLETDLKIMRDIVLTDQEKAELMAEASIPGLYCNACSNCVPGCPNNLPIPELMRAYMY
                     AYGYHNAAMASELVSETAVESNPCAGCDTCTAHCVRGFDIRAKISDISRLANVPYDLL
                     A"
     gene            3378..>3920
                     /locus_tag="PLA17_10050"
     CDS             3378..>3920
                     /locus_tag="PLA17_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK85794.1"
                     /translation="MGRYIHNLISGGEGLNLDFKYCISDPQKIARTLSAFSNTAGGKL
                     LIGVRDNGSLAGVRSDEEYYMIDAAARLHCDPEVTIRTRNHTINGKNILEAEVLKSPV
                     IPVKARDEHGRWRAYFRQNDQNFMADRVILQVWRRSAKSRGLLLRFEETENLLLSFLR
                     SEGRITVKEFRILAGINARRA"
BASE COUNT         1040 a          987 c          943 g          950 t
ORIGIN      
        1 aacagggccc tgttccgcaa aatagaccag tttgagaaag atgttatctc aggtaagatc
       61 aatatcagac aggactcggt gagatctgca atcagccagc ttaaaaagga tgatgataca
      121 gggcctatcg ttcttatcaa agcctacaag acagccaagt acaaaaattt cgtcgatatc
      181 attgacgaga tgtcaattgt gggtattgcc cgatacattt ttacggatat agactggata
      241 gaagagaatc tggttgttga tgccctcaac agggcagcag ccgctccttc aggttcagca
      301 caatagggag gtacaactat ggcaaataaa aggaaacgat acccaagctt cgatgatata
      361 gtattcgaag gcagaaacaa ggaatacggc gcatacgaac tccgcaggaa atattccagg
      421 acaatgacag tatcaattat tgttggagta attgtggttt tcatcgcagc ctatgttccc
      481 tatctgaaag ccagaagtat agctcatgtc aaggagagag acgctactga ggtcatagct
      541 gaaatggcta atgacatcaa ccaggaagcc gctgctcctc caccgcctcc accgcctcca
      601 ccgccgacag aacagcagac agtggtgaaa tacgtggctc ctgtaatcgt tgatagcatc
      661 aaacctgaag aggcatcaca gtttatggct atggacgacg tggctgaaac tgtggttgac
      721 gaggatgtag ggcttgaggt aattgaacag gtacaggaag agatagagga agaagcccca
      781 caagaggtgt ttgtggtggt tgaagagatg ccctcattcc cgggtgggga tgccgagctc
      841 ttcaatttca tctacagcaa catcaattat cctgagattg ccaaggaaaa caacatccag
      901 ggcagggtaa ctcttcgctt ctgcgtaaca tacaagggta ccgttgatca ggtatccgta
      961 gtcaggggcg ttgacccgtc gctcgatgaa gaggcaatca gggtcatcaa gatgctcccg
     1021 ctgtggaaac ccggcaaaca gggcggtaaa cccgtgaacg tatggtatag cgtacctatc
     1081 aaattcgagc tcaagtaaag gaagcttaac gaaaattaaa aaggtgtctc aattgtgagg
     1141 cacctttttt attgcgatcc atcaaatact ctgtgttcag tggctgccaa gcattaacgt
     1201 gatgtaacgc tcagccagag acgacctctc caatggcagc ccgctgatca gtatcagctt
     1261 tcccccctgc ctcgccatat acagcaaccc gttgaatcca tccttaaagg catacctgaa
     1321 aacctcatca tcggctgccc atgcatctcc ggcaaggctg gctgccattg ctgcaacttc
     1381 agccggatct tcacggctga agagatatat gtcaaaccgg tctccctcaa cctcgtacga
     1441 agccctgaag gcgccgcgca gaaactcgtg ccccagaaca ctctccagca ggtatgtctc
     1501 ctgattctca agaagccctt cggaaggaaa gagcctgagc agtgccggaa actcacccga
     1561 tccgcccata cgggacgaaa tcagagtggc aagatcaagc atggagctgt tcacttttgc
     1621 agagagggaa tggctcatga tcttgacata aaaacagtct ttgtagaagt gaaccacacc
     1681 ttcctcattg tacccctgta cccccgtgtt aatgaattta taccctggcg agcgctccat
     1741 ggagtacatt ccgaaagccc tggcatcatc accaaaccgg tagatttccg ccttgatgat
     1801 gttcttgccc ttaacatact cccttatgtt caggtcaatg aaacccagct gaaggtaggc
     1861 atcggctgca ccgttgatat agttccaaag gtcgtcagga gtgtaaacag gatagtcatc
     1921 actgatcttg tatcccctca actccgggaa aagaccctga ccctgaatgg tctgaaatga
     1981 gacaatgagc agtacggaaa taaaaagggt tcttttcatc gcgttgttat tttatgccag
     2041 aaggtcatat gggacattgg ccagcctgga aatgtcactg atcttggctc tgatatcaaa
     2101 tccccttacg cagtgtgcag tgcatgtgtc acagccggca cagggattag attcaaccgc
     2161 agtctcactt acaagctctg atgccattgc ggcattatga tagccgtagg cgtacatata
     2221 agccctcatc agttcaggta tcggcaggtt attgggacag ccggggacac agttgctgca
     2281 ggcattgcag tagagcccgg gtattgaggc ttcagccatc agttcagcct tctcctgatc
     2341 tgtgagaaca atgtccctca taatcttcag gtctgtctcc aactggtcaa atgcagtaat
     2401 accggggatg gtggtatgca cattcggatt tgacaatacc cacctcaggg cagcctgtac
     2461 gttgacgggc ttagttctct ccctgtcaag ccatcctccg gccagggttt tcatggctac
     2521 cacacccata cccgctgctg ctgctttctc aatggcgctg ttcatctcat caatgtatgt
     2581 ctggcggtag ttgtacgagg taagcacaac atcccagata ccctcctcaa ccatggtatt
     2641 tataaccagc ggctcattct tgtgtgttga tatgccaatg aaccgaaccc ttccctgctt
     2701 cttcagatca agcattgctt tgacagtttc cttgtgcctg accatctcag cagtgtcagc
     2761 agagtgcata tatagtatat caacgtagtc aactttaagc ctgctgaggc ttatatcaaa
     2821 catctcaagg aatctctccg cagtggtatc ctttgagggg agacctgtac ggtcaacccc
     2881 tgccggtttc accttggtgg ccaatacaaa tgattcacgg ggcaccgtgc tgaagaagtt
     2941 tccaagcatc tcctcattcc tgccgttcat atagccgtgg gccgtatcaa aaagcttcat
     3001 gccaccctca taggcagctt ttaccagtgc gctgttgtca gccctcatca cgccgaaact
     3061 gactacgggc agcctgagac cggtcctgcc aagggtcctg tatacaatct ctccctcatc
     3121 tcttttgctg tttccggcca gggcccggtt acccatcaat gtgatccctg ctgctcccgc
     3181 aagaccggat ctgaaaaagt ttctgcgttt catgaagaat gttttgttgt ttaatcttat
     3241 tactgcacat attctcactt cacggcagtg ttgataaatc cgttctgacc cggtcaccgt
     3301 gccgttgaga atgaaccaat atacaaaatt ttattatatt ttagcgaggc tgctgcgcag
     3361 cataaaatca ggcaattatg ggacgatata tccacaacct gatatcaggc ggcgagggtc
     3421 tgaaccttga cttcaagtac tgtatttctg atccgcagaa gattgcccgt accctgtcag
     3481 cattttcaaa cactgccggg ggtaagctgc ttatcggggt cagggataac ggatcactcg
     3541 ccggtgtgag gtcagacgag gagtattaca tgatagacgc cgccgcaagg ctccactgtg
     3601 accccgaggt gactatccgc acaagaaacc acactattaa cggcaagaat atccttgagg
     3661 cggaggtgct gaaaagcccc gtaattccgg taaaggccag agacgaacat ggcaggtgga
     3721 gagcctattt caggcaaaac gatcagaact tcatggctga cagggtgatc cttcaggtgt
     3781 ggcgccgtag tgcaaagtca cgcggactgc tactgaggtt tgaggagact gaaaacctgc
     3841 ttctgagttt tctgcgcagc gagggcagga taacggtgaa agaattccgc atcctcgcag
     3901 gtatcaatgc aaggagggcc
//
LOCUS       DAOPBK010000206         3880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_19062_length_3880_cov_4.638170, whole genome shotgun sequence.
ACCESSION   DAOPBK010000206 DAOPBK010000000
VERSION     DAOPBK010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19062_length_3880_cov_4.638170"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLA17_10055"
     CDS             <1..982
                     /locus_tag="PLA17_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85795.1"
                     /translation="LLIPTRQAFSIYDVDRLVSSSESSALAISRLVFSGSNRNATLFP
                     QSDEEKMVPGKANNLTVYIANPSGFDREVREYLYRITELGEEWYRTATDNFSLLNLKH
                     GEYHLQVKEAAGRGMTETCFTIRRPFALTGWAMLLYLLTIAAVTAAAIMFFRSKLEGH
                     RRMIEYEVGKNRLESELDYKSYELMLTMRYLIRKTDTLRELRDKLETAKESSLKMPVR
                     FIREMEQIIDHGLDTQTEEWQNVMKNLKLSQEGFFRKLKARYPALTPNDLRLCSYLRM
                     NFTTKEIANLSNISTRSVEIARYRLRSKLNLSHEVNLTEFLIHEAEISED"
     gene            1373..2551
                     /locus_tag="PLA17_10060"
     CDS             1373..2551
                     /locus_tag="PLA17_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPK85796.1"
                     /translation="MKKVSLWLVFIAITAVSCGPRSPRGEGGRVVTVTIPPFAWFVEQ
                     IGGDDFTVNVLLPPGADHHIWEPLPVQISSLSASEAFIMNGQLGFEHAWTDRFMQVNP
                     DMKVLDLSRHIELIGAEGLRLESHGSDGSGSDGIRSGSSGSEGNRSDGVRYGSSGSGG
                     PAEGHGAGEHAGEGHNDEAEGYGDEGAHAGEGHNGHARNDGGPKEHALRDAGKDHIRA
                     AGDHGHSHAGPDPHYWMSPSAAYIIARDVHDFLVGLNPASSEKYDSNLSAVNRKISEV
                     DSLARVALGSDPHPMVMIYHPALAYMGRDYGFEQVSFEDEGKSPSPARMKELIDLARD
                     NGIKIIFIQAEYDVRNAESLSRETGARLVVINPMNSNWPEAVIEVARAMGDGGTGVKK
                     "
     gene            3023..3769
                     /locus_tag="PLA17_10065"
     CDS             3023..3769
                     /locus_tag="PLA17_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPK85797.1"
                     /translation="MSVLFAIESLTAGYEGKIVLSDVSMIVQENDFIGIIGPNGGGKT
                     TLLKVILGLLQPLSGKVDYSPGNLRLNQIGYMPQVAQGDTLFPVTVEDVVLSGMMHNR
                     GPTGRMGRAEKKHAAEVMDELGLSPLAHQALNELSGGQLQRVYLARAISGSPRLLLLD
                     EPATFVDASFETDFYEKLKLLNSRMTIMMVSHDVGTISAWVKSFACVSGKVFYHPHSK
                     ITEEDLRMYGCPIQLVTHGDVPHVVLHKHD"
     gene            3769..>3880
                     /locus_tag="PLA17_10070"
     CDS             3769..>3880
                     /locus_tag="PLA17_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPK85798.1"
                     /translation="MENLFEYSFVLRSLLGALFASITAGVAGTYIVSRRLV"
BASE COUNT          969 a          865 c         1152 g          894 t
ORIGIN      
        1 gcttctcatc cccacgaggc aggcattcag catctatgac gttgacaggc tggtcagcag
       61 ttcggagagc tctgcgcttg ccatcagccg tctggttttc agcgggagca acaggaacgc
      121 aacactgttc ccgcaatcgg atgaagaaaa aatggtgccc ggcaaggcca acaacctgac
      181 cgtctatatc gcaaatccat caggattcga ccgcgaggtc agagagtatc tttacaggat
      241 caccgagctt ggagaggagt ggtatcgaac agctactgac aacttctcac tgttaaacct
      301 gaagcacggt gaatatcatc tccaggtaaa agaggcggcc ggcaggggca tgacagagac
      361 ctgttttact atcaggaggc cctttgccct gaccggatgg gccatgctgc tctacctgct
      421 tactattgca gctgttaccg cagccgcgat tatgtttttc cgttccaaac tcgagggcca
      481 ccgccgtatg attgaatatg aggttggcaa gaacaggctg gaaagcgagc tcgattacaa
      541 gagttatgag ctgatgctga ccatgcgtta tctcataaga aaaactgaca ccctcaggga
      601 actgcgtgac aagctggaga cagccaagga gagctcttta aagatgcccg tccgtttcat
      661 cagggagatg gaacagatta ttgatcacgg acttgacacc cagacggagg agtggcagaa
      721 cgtgatgaaa aacctgaagc tgtcacagga agggttcttc agaaagctga aggccaggta
      781 tccggcactg acacccaatg acctcaggct atgttcgtat ctgaggatga actttaccac
      841 caaggagatt gcaaatctct cgaacatatc cacacggtcg gtagagatag cgcgttacag
      901 gctgagatca aaactgaacc tcagtcatga ggtcaacctg acagagtttc tcattcacga
      961 ggcggagata agtgaagatt aggttccggt gaggccatgc ctgtctggat gaaggacgga
     1021 gtccctgcca ctgaaaagga gttcaccctg ggatactgac agctaccgct ttcacgggat
     1081 aattggtttt aacgggcact tcccgactgg tggtgccggg cttccgggct gtcaggggtg
     1141 ccggaattgt tactgcgatt ccggtgctcg acttatttac gcagttttct cgcttgtacg
     1201 tggcgccggg cttccgggct gtcaggggtg ccggtcagat ccgatttcct gcctcccagg
     1261 gaagcctggt tcggggactc tgttgataac atcatgttga acagtttcgt ccctgaatcg
     1321 ttttaccctt ggataccata aattgttaat tttgcattgc acaaaaagag gcatgaagaa
     1381 ggtttcattg tggctggttt ttattgcaat aaccgcggtt tcctgcggtc cccgctcccc
     1441 gcggggggaa ggaggcaggg tggtcacggt gaccatacct ccgtttgcat ggtttgttga
     1501 gcagattggt ggagatgatt ttacagtgaa tgtgctgctg ccaccggggg ccgatcacca
     1561 tatctgggag cctcttccgg tgcagataag ctccctgtca gcttcagaag cttttattat
     1621 gaacggtcag cttggtttcg agcatgcatg gacggaccgt ttcatgcagg tcaatcccga
     1681 catgaaagtg ctggatttgt cacggcatat tgagctgata ggggctgaag gtttaaggct
     1741 tgaaagtcac gggtcagatg gttccgggtc agatggtatc aggtctggca gttccgggtc
     1801 tgagggtaac aggtctgatg gtgtcaggta tggcagttcc gggtctggcg gcccggcaga
     1861 agggcatggc gcaggtgagc atgcagggga aggccataat gatgaagcag aagggtatgg
     1921 tgatgaaggt gcgcatgcag gagaagggca taacgggcat gcccggaacg atggagggcc
     1981 taaagagcat gccttgcgtg atgcagggaa agatcacatc agggctgccg gtgatcatgg
     2041 tcacagccat gccggtcccg acccgcatta ctggatgtcg ccctctgctg catatataat
     2101 agcccgtgat gtgcatgatt tcctcgtcgg gctcaatccc gcctcatcag aaaaatatga
     2161 ttcaaacttg tctgcggtca accgcaagat atcggaggtt gattcgctgg caagggtggc
     2221 tctgggatct gacccgcacc cgatggttat gatctatcac ccggcgttgg cctacatggg
     2281 tcgtgattac gggtttgaac aggtatcctt cgaagatgag ggcaagagtc cctctccggc
     2341 gaggatgaag gaactgattg accttgcccg ggataacggg ataaagatta tcttcataca
     2401 ggcggagtat gatgtacgca atgctgagtc gctctcgcgt gagaccggtg ccaggcttgt
     2461 ggttatcaat cccatgaaca gtaactggcc ggaggctgtc atagaggtgg ccagggcaat
     2521 gggcgatgga gggacgggag tgaaaaaatg aaccggtgag tcattgtagc agaggaaccg
     2581 gtttgttgtg agggcaataa tattggcaat ggagggacag gtgagaagaa atgacccggt
     2641 gggtgatgtt agcgaaggag ccggactgca gtgagggtgg tggtgttggc aatgaatgct
     2701 cgcaggcaat gacaagcagg aaatggtgtt ggcaatgaat gctcgcagag aatgttcaga
     2761 aggtaatagt gttgacaatg aatactcgca gagattgttc agaaggtaat agtgttgaca
     2821 atgaatactc gcagagaatg ttcagcaggt aatagtgttg acaatgaata ctcgcagaga
     2881 atagccagcg gacaatagtg tgttaatgaa tgctcacata gaatggtcgg cggacaatag
     2941 gatatcaaat aaatgcccga ggcacaaggc agcaggtggc agtgttgaca atgatgaata
     3001 cctgagcaat aacagtttaa atatgagtgt attatttgca atagaatcat taacagccgg
     3061 gtacgaggga aaaattgtcc tcagtgatgt atcaatgatt gtacaggaga atgattttat
     3121 tggcataatc ggtcccaacg gcggaggcaa gacaacactt ctgaaggtga tcctcggact
     3181 gctgcagccg ctatccggaa aggttgacta ttcacccggc aacctgcggc tgaaccagat
     3241 aggctacatg ccccaggtgg cacagggcga caccctcttc ccggtgacgg tggaggatgt
     3301 tgtgctttcg gggatgatgc ataacagggg ccccaccggc cggatgggtc gggctgaaaa
     3361 gaaacatgct gcggaggtga tggatgagct cgggctgagt ccgctggcac accaggctct
     3421 gaatgagctg tcgggcggac aactgcagcg cgtctatctt gcaagggcca tatcaggctc
     3481 accacggttg ctgctgcttg atgagcctgc aacatttgtg gatgcctctt ttgagactga
     3541 cttctatgag aaactgaagc tgcttaacag ccggatgacg ataatgatgg tctcgcatga
     3601 tgtgggcacc atctccgcct gggtgaaatc atttgcctgc gtaagcggaa aggtgttcta
     3661 tcacccgcac agcaagatca ccgaagagga tctcaggatg tacggatgcc cgatacagct
     3721 tgtaacccac ggtgatgtgc cacatgtagt actccataag catgactgat ggaaaacctc
     3781 tttgaatact catttgtgct caggtcgctg ctgggggcgc tgtttgcaag catcacggca
     3841 ggagtggcag ggacatacat cgtctcaagg aggctggttt
//
LOCUS       DAOPBK010000207         3816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_19457_length_3816_cov_4.203669, whole genome shotgun sequence.
ACCESSION   DAOPBK010000207 DAOPBK010000000
VERSION     DAOPBK010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19457_length_3816_cov_4.203669"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PLA17_10075"
     CDS             <1..1023
                     /locus_tag="PLA17_10075"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK85799.1"
                     /translation="HTLRRKGIDISAEEEEGVLAAILLHDIGHGPFSHALEHSLIEDM
                     PHEAMSLLIMEELNKTMGGRLTTAIAIFRGEYPRRFFHDLVTGQMDMDRMDYLRRDSF
                     FTGVIEGSVGSDRIIQMLNVSDDRLVVDEKGIYSVEKFLIARRMMYWQVYNHRTVVSA
                     EKLITNLLKRARQVTAAGVKLFATPALAYFLEPRNNRAEGEERASLVGHFTALDESDI
                     ISATKVWMNCGDRVLEELCRRFVNRDLLAIELQRTPFHPARVSETARMAKEVLGLTDD
                     EVGYLVNTGDVYNQTYAPGTPEVRILLKNGTTQDITAVSDLFDKDALSEKVTKYYLCY
                     PKEILK"
     gene            1036..2073
                     /gene="lpxD"
                     /locus_tag="PLA17_10080"
     CDS             1036..2073
                     /gene="lpxD"
                     /locus_tag="PLA17_10080"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPK85800.1"
                     /translation="MEFTAAMIARMLNGTVDGDPDTKLNTVARIEEGVEGALSFLSNM
                     KYEPYLYTTAASAVLVSNDFTPAKEVRATLIRVDDPYQALARLLAFYEQARPAKKGIH
                     ASAVIDEKASVGADVFIGACAVISENVVIGDGCSLYPQVFIGENVKIGRNCTLYPGVK
                     VYRDSVIGNDCIIHAGAVIGADGFGFAPVTDANFMKIPQIGNVILEDNVEIGANTCVD
                     RATMGSTIIRKGVKLDNLIQVGHNCEIGENTVIAAQSGLAGSTIVGRNCMFAGQVGLA
                     GHLRIADGVKIGAQSGIMSTISEENATYLGYPATNSKQFLKTTVYLRKLPDLSNRVDA
                     LEKAVEALKNS"
     gene            2185..3579
                     /locus_tag="PLA17_10085"
     CDS             2185..3579
                     /locus_tag="PLA17_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPK85801.1"
                     /translation="MSEKQRTLVNEVSLSGKGLHSGIDVNVTFKPAPPNHGYKFCRTD
                     LPGAPLIDAIAENVTETSRGTTLVQGEASVATIEHILAAFAGMRVDNALIEINGPEAP
                     IMGGSSRMFVEAIKKAGIVEQEEERNYYAIREKITFSDEEHGVDLIIYPDDRFSAHVL
                     IDYNSKILGNQYAILDQIDDFEKEIAGSRTFVFFHELEPLFRMGLIRGGDLENAVVIL
                     EREVEQEEIDRIAKMFNKPGITKHNAGVLNNTKLRYPNEPARHKLLDLMGDLALVGQP
                     IKGKVVATRPGHYSNTRLARLIRQQIKRQATKKEIPLLDTTRPAVMDIQQIKKLLPHR
                     FPFLMVDKILELSTNHVIGMKNTTYNEAFFQGHFEQEHVFPGVLLVETMAQVGGILVL
                     HNVDEPERYSTYFLKIERLKFRHKIIPGDTIIVRADMTEPIRRNIVIMYCQAFVGEKV
                     VAEGEMTAQVIKNK"
     gene            3586..>3816
                     /locus_tag="PLA17_10090"
     CDS             3586..>3816
                     /locus_tag="PLA17_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HPK85802.1"
                     /translation="MISSLAHIDPKAQIGEGVTVEPFVRIYGDVVIGDGTWIGSGAVI
                     YDGARIGRNCLIFNGASIAAIPQDMKFSGEETT"
BASE COUNT         1050 a          828 c         1079 g          859 t
ORIGIN      
        1 cacacactca ggagaaaggg tattgatatc tcggcagagg aggaggaggg ggttttggca
       61 gccatactgc tgcatgatat cggccacggg ccgttctcac atgctcttga gcactcactg
      121 atagaggata tgccccatga agccatgtca ctccttatca tggaggagct caataaaacc
      181 atgggaggaa gactgacaac tgccatagcc attttcaggg gagagtaccc gaggagattt
      241 ttccatgatc tggtgacggg gcagatggat atggacagga tggattacct cagacgagac
      301 agtttcttta ccggggtgat agagggttct gtggggtcag acaggattat tcagatgctg
      361 aacgtctctg acgacaggct ggtagtagat gaaaagggaa tatactccgt agagaagttt
      421 ttgatagcca gacgtatgat gtactggcag gtctataacc accgcaccgt tgtctcagct
      481 gagaagctga tcaccaacct tctcaaaagg gcgcgacagg tgacggcggc aggagtgaag
      541 ctgtttgcca cgccggcgct ggcctatttt ctggagccgc gcaacaacag ggctgaaggg
      601 gaggagaggg catcactggt ggggcacttt accgctctcg acgagagtga cataatctcc
      661 gcaacaaagg tctggatgaa ctgcggtgac agggtgctgg aagagctctg caggagattc
      721 gttaacaggg accttctggc aatagagctt cagagaaccc cgttccaccc ggcaagggtg
      781 agtgaaacag cacgcatggc aaaggaagtg ctcggactga ctgatgacga ggttggctat
      841 ctcgtcaaca ccggcgatgt gtacaaccag acatacgctc ccggcacccc tgaggtacgg
      901 atattgctaa agaacggtac cacacaagat attacggcgg tatctgacct ctttgacaaa
      961 gatgcgctgt cagaaaaagt gacgaaatat tatctgtgtt accccaagga aatactaaaa
     1021 taaaaaatat accagatgga atttactgct gcaatgatag cccggatgct gaacggtacg
     1081 gtggatggcg atccggacac gaaactcaac acagtggcca ggatagaaga gggcgtggag
     1141 ggtgcccttt cgttcctttc aaatatgaag tatgagcctt acctgtacac aacagccgct
     1201 tctgctgtgc tggtgagtaa tgatttcact ccggcgaagg aggtcagggc aacccttata
     1261 cgggtggatg atccgtacca ggcgcttgcc agactgctgg ctttctatga acaggccagg
     1321 ccggcaaaga aggggataca tgcctcggca gtaatagatg agaaagcctc ggtgggagcg
     1381 gatgttttca tcggtgcctg tgccgtcatc tcggaaaatg ttgtcatagg tgacggatgc
     1441 tcactttatc cccaggtgtt tataggggag aatgtcaaaa tcggcaggaa ctgtacactt
     1501 tatcccggag tgaaggtgta ccgtgatagt gtgatcggta atgattgtat tatccatgca
     1561 ggggcagtga taggcgctga cggatttggc tttgcgcccg tcaccgatgc caacttcatg
     1621 aagataccac agataggcaa tgtaatactg gaagataacg tggagatcgg ggccaacacc
     1681 tgcgtagacc gtgctaccat gggctcaacg attatcagga agggggtcaa gcttgacaac
     1741 cttattcagg tgggccacaa ttgcgaaata ggggagaaca ccgtcatagc cgcccagtcg
     1801 gggctggcag gatcgactat cgtgggaaga aactgcatgt ttgcagggca ggtgggtctg
     1861 gcaggacatc tgcggatagc cgatggtgta aagatcgggg cacagtcggg cataatgagc
     1921 acaatcagcg aagagaatgc cacttacctc ggataccctg caaccaacag caaacaattc
     1981 ctgaaaacaa cagtttatct tagaaaactt cctgatctga gcaacagggt tgatgctcta
     2041 gaaaaagcag ttgaagcttt aaaaaacagc tagttccgaa aaacggtcta aaggaaatga
     2101 tttgaacatc acacagatgt aaggtttttg attatgccgt tcggaaaaaa ttacttttct
     2161 ttgcccaaat aataatgacc tgttatgagt gaaaaacaaa ggacgttggt taatgaggtg
     2221 agtctcagcg gcaagggtct tcattccgga atagatgtaa atgtgacttt caagcctgct
     2281 cctccgaacc atggctataa gttttgtcgc actgaccttc ccggtgcgcc gctgatagat
     2341 gcgattgctg aaaatgtgac tgagacatca aggggtacga ctctggtgca gggcgaagcc
     2401 tccgttgcaa ccattgagca tattcttgca gcatttgcgg gaatgcgtgt tgacaatgcg
     2461 ctgattgaga taaacggtcc cgaagccccg atcatggggg gcagttcccg catgttcgtt
     2521 gaggcgataa agaaggccgg aatagttgaa caggaagagg aacgcaacta ttatgcaatc
     2581 agggaaaaga taactttctc tgatgaggag cacggagtgg acctgataat ctaccccgac
     2641 gatcgtttca gcgcccatgt gctgatagat tacaactcga agatacttgg caaccagtat
     2701 gccatccttg accagattga tgactttgaa aaggaaatag ccgggagccg tacatttgtc
     2761 ttcttccatg agcttgaacc ccttttcagg atgggactta tcaggggggg ggatctcgag
     2821 aatgccgtgg tgatcctgga acgtgaggtg gagcaggagg agattgaccg cattgcaaaa
     2881 atgttcaata agcccggtat aacgaaacac aatgccggtg tgctaaacaa tacaaagctc
     2941 aggtatccca atgagccggc caggcacaag ctgcttgacc tgatgggtga ccttgccctg
     3001 gtagggcagc ctatcaaggg aaaggtagtg gcaacccgcc cgggtcatta ttccaatacc
     3061 aggcttgcca ggctgatcag gcagcagata aagagacagg ctacgaaaaa ggaaatacct
     3121 cttcttgata ctacacggcc ggcggtgatg gacatacagc agatcaagaa gctgcttccc
     3181 caccggttcc cgtttctaat ggttgacaag atacttgaac tcagcacgaa ccatgtgatc
     3241 ggaatgaaga acacaaccta caacgaagcc ttctttcagg gtcatttcga gcaggagcat
     3301 gtcttccccg gggttttgct ggtggagacg atggcgcagg tgggaggcat tctggtgctg
     3361 cacaatgttg atgagcccga aaggtactcg acctatttcc tgaagattga aaggttgaag
     3421 ttcaggcata agattatacc gggtgatacc attatagtaa gggcagatat gaccgaaccc
     3481 atacggagga atattgtgat catgtactgt caggccttcg ttggagaaaa ggtggttgct
     3541 gaaggagaga tgacggcgca ggttataaag aacaagtaac aggatatgat ttcttcactg
     3601 gcacatattg atccgaaggc tcagattggc gagggggtta ctgttgagcc atttgtcaga
     3661 atctatggtg atgttgtcat tggcgacggt acctggatcg gctcgggggc ggttatctat
     3721 gacggcgcga ggataggcag aaactgcctt atctttaacg gtgcctcaat cgctgcaata
     3781 ccgcaggaca tgaaattcag cggagaggag accact
//
LOCUS       DAOPBK010000208         3781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_19667_length_3781_cov_4.580247, whole genome shotgun sequence.
ACCESSION   DAOPBK010000208 DAOPBK010000000
VERSION     DAOPBK010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19667_length_3781_cov_4.580247"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLA17_10095"
     CDS             complement(<1..360)
                     /locus_tag="PLA17_10095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653874.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPK85803.1"
                     /translation="MDNFIVSARKYRPATFDMVVGQDTITTTLRNAIRNNLLAQAYLF
                     CGPRGVGKTTCARIFAKTINCPDTGPDGEACDKCESCRSFNSQASFNIHELDAASNNS
                     VEDIRNLTDQVRIPPQIG"
     gene            497..847
                     /gene="rpsF"
                     /locus_tag="PLA17_10100"
     CDS             497..847
                     /gene="rpsF"
                     /locus_tag="PLA17_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPK85804.1"
                     /translation="MNQYETVFIATPVLSENQMKEAVQKFKKIITDNEGEIVHEENWG
                     LKKLAYPIQKKSTGFYYLIEFRGPGELIDKLEVQYRRDERIIRFLTFRMDKYAIEYAE
                     KKRKMKETEKVREE"
     gene            850..1119
                     /gene="rpsR"
                     /locus_tag="PLA17_10105"
     CDS             850..1119
                     /gene="rpsR"
                     /locus_tag="PLA17_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPK85805.1"
                     /translation="MAQGSSEIRYLTPPTVEIKKKKYCRFRKNKIKYIDYKDPEFLKK
                     FLNEQGKILPRRITGTSLKYQRRVARAIKRSRHLAMLPYVTDLLK"
     gene            1132..1578
                     /gene="rplI"
                     /locus_tag="PLA17_10110"
     CDS             1132..1578
                     /gene="rplI"
                     /locus_tag="PLA17_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPK85806.1"
                     /translation="MKIILRQDIEKLGQKDDVVTVKTGYARNYLIPNGLAIQATDSAL
                     KIHNENLKQRAHKEEKIRGEAEELAKKLAEVKLVIGAKVSSAGKIFGSVNNIQLAEAL
                     NEKGYEIDRRNILLGDDAIKEVGNYKAVVKLHRDVKVDLEFEVIAE"
     gene            complement(1758..2138)
                     /locus_tag="PLA17_10115"
     CDS             complement(1758..2138)
                     /locus_tag="PLA17_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPK85807.1"
                     /translation="MKRIISTTGAPGAIGPYSQAVEAGGTLYISGQVPIDPATGKLVE
                     GGIAEQTRQVLKNIRAILTAAGYTMNDVVKSTCLLSDMSDFKAMNEVYAQFYTADQPA
                     RAAFAVKGLPLGALIEIETVAVKE"
     gene            complement(2158..>3781)
                     /locus_tag="PLA17_10120"
     CDS             complement(2158..>3781)
                     /locus_tag="PLA17_10120"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPK85808.1"
                     /translation="SSSGYDKNNSYEVADHVTTTRQSSISYSKTWSGAPVSFSTSINQ
                     SQNVQNKTMMLNLPRGSLNVSRIYPFKPKKLVGKSKWYHDITTQYTASFENKINTYDS
                     LLFTSAVWKSMQNGFKHEIPLSLQFRPFNNFSISPSLRYTGVLYTQQIHKRWDPDYYD
                     ERRNMVVPSVVNDTVRGVTYGHALVPTVSASFNPSIYGTFAFTKKGSGIEAIRHVMKP
                     SVSFSYSPEADKLSSDMYRTVQYDTVGRMRTYSIYEGSVFGTPSTGNRSGSVAFSLSN
                     IVEAKVYSRNDTTGKPRKIKLIDNLSLNTSYNLFSDSLNWSPVNISFRTTLAQNINIQ
                     ASSSFSIYGMNATGGAVNELAIRQGLGLARMTGFNMSFDLDLGQLLGNKNKKQQSGGQ
                     GGQMSTGRQPGGPGAQGGAAPDNLPLSNRNLDEFGYVRFDVPWSLRMAYNFSYSKPGL
                     RTNISQTMTLRGDLKLTPKMALTYNTGYDFRQKEITMTRVGISRDLHCWEMSFSWIPT
                     GSMKSWNFTVRAKASMLQDLKYERRKDYHENY"
BASE COUNT          977 a          857 c          946 g         1001 t
ORIGIN      
        1 tcctatctgc ggaggtatcc gcacctggtc agtcaggttc cggatgtctt caacactgtt
       61 gttcgatgca gcatcgagct catgtatatt gaaggatgcc tgtgaattga atgacctgca
      121 tgattcgcac ttgtcgcatg cttcgccgtc aggcccggta tccggacagt ttatggtttt
      181 ggcgaagatt ctggcacagg ttgtttttcc aactccgcgg gggccgcaga aaagatatgc
      241 ctgggccaga aggttattcc tgatcgcatt acgcaaagtg gtggtaatgg tatcctgccc
      301 tacaaccata tcaaaggtgg caggtctgta tttgcgggca gaaacaataa aattatccat
      361 accgggtttt aacagtatat gtacatatcc gtcacaaata tagtcaaata gttttcggaa
      421 tatttttttt aaagatttat tgttttactt ttgcaaccca aatttattaa caccaataca
      481 aaccaattaa gagatgttga atcagtacga aaccgttttc attgcaactc ccgttttgtc
      541 tgagaatcag atgaaggaag cggttcagaa gttcaagaag atcatcactg acaatgaagg
      601 tgagattgtt catgaagaga actggggtct gaagaaactc gcttatccca ttcagaaaaa
      661 atcgaccggg ttttattacc tgatcgagtt cagggggccg ggtgagctca ttgacaagct
      721 ggaagttcag tacaggagag atgaaaggat catccgtttc ctgacattcc gtatggataa
      781 gtatgctatt gagtatgctg agaaaaagag aaagatgaaa gaaaccgaaa aagtcaggga
      841 ggagtaatca tggcacaagg cagttcagaa atcaggtacc tgacaccacc tacagtagag
      901 atcaagaaga agaaatactg caggttcagg aagaacaaga tcaagtacat cgattacaag
      961 gatcccgagt ttctaaagaa atttctcaac gagcagggta agatacttcc ccgccgcata
     1021 acaggcacct cgctgaaata ccagaggagg gttgccagag caatcaagcg ttcgcgtcat
     1081 cttgccatgc ttccgtatgt aactgatctt ttaaagtaag gaggaagtga tatgaagatt
     1141 attctcaggc aggatataga gaagctggga cagaaagacg atgttgttac cgtcaagacc
     1201 ggctatgcaa gaaattacct tattcccaat ggtttagcca ttcaggcaac tgactccgcg
     1261 ctgaagattc acaatgagaa tctgaaacag agggcccata aggaggaaaa gatcaggggt
     1321 gaagctgaag agcttgcaaa gaagcttgct gaagtgaagc ttgtgatcgg agccaaggtc
     1381 agctcggctg gcaagatctt cggatcagtc aataacattc agctggcaga agcccttaat
     1441 gaaaagggat acgagattga ccgcagaaac attctccttg gcgacgatgc catcaaggag
     1501 gtaggaaact acaaggcggt tgtcaaactg caccgtgatg taaaggtgga tctggaattc
     1561 gaggttattg ccgaataatt accacaaacc atattgatat tcatgcagcg acgggtctga
     1621 gacccgtcgt tacggaataa tgatcacata tctgcatggc aggatacaaa actgccaggc
     1681 gggaaggtgg cggcgggtct gagacccgcc gttacggaat cataaataca tatctgcctt
     1741 atgggcaggt gaataatcta ttctttaacc gccaccgtct caatctcgat aagggcgccc
     1801 agcggcagac ccttaacggc aaatgctgcc ctggcaggct ggtcagcagt gtagaactgc
     1861 gcgtaaacct cattcattgc cttgaagtca gacatgtctg acaacaggca ggttgacttg
     1921 acgacatcat tcatcgtata gcccgctgct gtcagtatag ctctgatatt tttcagtacc
     1981 tgtctggtct gttcggcaat acccccctct accaacttcc ccgttgcagg atcaataggt
     2041 acctgcccgg agatatagag ggtgccgccg gcctcaacgg cctggctgta aggtccaatt
     2101 gcacccggtg cgccagtagt gctgatgatt cttttcattc ctgatatctt tgattcgtta
     2161 gtagttttca tggtaatcct tcctcctttc atacttgaga tcctgaagca tgcttgcctt
     2221 tgccctgaca gtgaagttcc agctcttcat ggaaccagtg ggaatccatg aaaagctcat
     2281 ctcccagcaa tggagatcac gactgatacc cactctcgtc atggttatct ccttctgtct
     2341 gaagtcgtat ccggtattgt aggtgagagc catcttcggg gtcaacttca ggtctcctct
     2401 cagggtcata gtctgggata tattggtacg cagtccgggt ttgctgtagc tgaagttata
     2461 tgccattctg agtgaccacg gcacgtcgaa cctgacatat ccgaactcat caaggtttct
     2521 gttggagaga gggagattat ccggtgctgc tccgccctgt gctcctggtc cgcccggttg
     2581 acggcccgtg gacatctgtc cgccctgccc cccggattgt tgctttttgt ttttgttccc
     2641 cagcaactgc cccaggtcaa ggtcaaaact catattaaag cccgtcatcc tggcaaggcc
     2701 gagcccctgt ctgatggcaa gctcgttaac agcgcccccc gtggcgttca ttccatagat
     2761 cgaaaaactg ctgcttgcct gtatatttat gttctgtgcc agggtagtcc ggaaagagat
     2821 attgacaggc gaccagttca gggagtcgga aaacagattg taagaggtat taagcgacag
     2881 attatcaatt aatttaatct ttctgggctt accggtggtg tcattcctgg aatagacctt
     2941 ggcttctact atatttgaaa ggctgaaggc gactgatcct gacctgttac ctgttgacgg
     3001 ggtcccgaag acgcttcctt catagatgga ataggttcgc atcctgccaa cagtatcgta
     3061 ctgaactgtc ctgtacatgt cactcgaaag cttatccgcc tcaggcgagt aggaaaagct
     3121 gacggagggt ttcattacat gccgtattgc ctcaatccct gatccctttt ttgtgaacgc
     3181 aaaagttcca tagatggaag ggttgaatga tgcgctcacg gttggtacaa gtgcatggcc
     3241 ataggtcact cccctgacgg tgtcattcac caccgagggg acaaccatgt tccttctttc
     3301 atcatagtag tcagggtccc atctcttgtg tatctgctgt gtgtaaagca ctccggtata
     3361 gcggagagag ggcgagattg agaagttatt gaaaggtctg aactggaggc tcaggggtat
     3421 ttcatgcttg aacccgttct gcatactctt ccagacagca ctggtaaaaa gaagactgtc
     3481 ataggtgttg atcttgttct caaaagaggc cgtgtactgg gttgtgatat catgatacca
     3541 cttgctcttc cccaccagct ttttaggctt gaaggggtag atgcgcgaga cgttcaggct
     3601 tccccttggc aggttgagca tcattgtctt gttctgcacg ttctgcgact ggttgatgct
     3661 tgtggaaaag cttacaggtg ctcctgacca tgtttttgaa tagctgatgc tcgactgtct
     3721 ggtagtggta acgtggtctg ccacttcgta gctgttgttc ttgtcatagc cgctggagct
     3781 c
//
LOCUS       DAOPBK010000209         3733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_19977_length_3733_cov_3.517673, whole genome shotgun sequence.
ACCESSION   DAOPBK010000209 DAOPBK010000000
VERSION     DAOPBK010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19977_length_3733_cov_3.517673"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2300
                     /locus_tag="PLA17_10125"
     CDS             <1..2300
                     /locus_tag="PLA17_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK85809.1"
                     /translation="QFLKTTGASTGVTAGFATTASRGTDVRQIAVDNIETIEVVRGIA
                     GVENGDMLSGTVKVTMKKGRTPFTAKIKTDPGIKQFYAGKGLSLGDKAGTLNLDFDYT
                     QSFDDIRVKYKTFNRLNGGLIYNNNLFTESKPLILSLSVRGSQTLDISKRDPDMLDIE
                     NYESKDQSLAMNLNAKWAMNSAVLTNLNLVVSGDIQHQVGHEVTMEEIPSGAQPQPVA
                     YEPGENQVPILPSSYISDLTIDGRPYYYNAKLSGNRSFNIGAALNNIVAGIEYRLIGN
                     NGLGRIYDLARPPVPVGGTVARPRSYKDIPSLGQLAYYAEENLGIPIGKTRLDLQAGI
                     RFTNVQPDGLFRSREHTTMLDPRFNARFTVIDRGQGIISNLALRGGYGLFSKAPSLLY
                     LYPDRAYWDKTGLSYYDPIAQRGLFIVTTKIFENPRNENLNPAVNRKIEGGFDLKVKD
                     LSLNVTLYSEKMDNGFSFESYYENFIFNRYIQPLQNGLDLYFVPGSGVFYIDPDSGDE
                     VQLPVVQDTVFVSFTYPGNGTMTTKRGVEFTLDLGTVNALQTSFIVDGAWMWVKRQTT
                     VNDLLKPSTGSIGGKEFPMVAVYPAGYGSIDKRFNTNIRTITHIRKLRMVASVTTQII
                     WLDCDQSVWDDPEGNPLFYTDPEVLDPYADKLFPKYVDPLGYYDRQMVYHEFDRSLAV
                     TKPYSDLIKRYNTPWFFAETDYPPYFVINFKLTKEITDKANFSFYANNITNHNPLLKQ
                     RGGAPEVYGRRNPPLYFGAEIKIKF"
     gene            2317..3522
                     /locus_tag="PLA17_10130"
     CDS             2317..3522
                     /locus_tag="PLA17_10130"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HPK85810.1"
                     /translation="MKKCTIFLVVLLAGMAALTSCKKGAQLYDVTIWAYYPEGYGKNY
                     ASDVDIVVKNTISGQEFNLVTGHQGFVEIQLEEGMYKVSATKETDDFNLNGITENLTV
                     QTTGLNNWEVHLVATSKAGGLVIKEIYYTGSKTESGNNYYSDQFHEIYNNSDELAYLD
                     GLCIALTYPIGSTPTEFVNEDGSIMDRIPCNFQTLMFPGTGQQYPLEPRTSVVLAMDA
                     IDHKTDPVGNPLSPVNLANAPFEAFIEAAGKDTDAPQATNMIVIYTTSPTMHDWLHSV
                     NGQGVILFRLPTGLDWETFVANPDNFMTKPGSSSPIEYLMVHKDWVIDGVECNRTEED
                     KRFKRLPTSVDAGMVWSLATYNSKSVRRKVEKILDGKVIYKDTNNSYNDFINDQVPTP
                     FVHPTTVDN"
BASE COUNT         1047 a          868 c          895 g          923 t
ORIGIN      
        1 tgcagtttct aaaaaccacc ggcgcctcaa caggcgtcac tgccggattt gccacaacag
       61 catcccgtgg tacagatgta aggcagatag cggttgacaa tattgagacc atagaggtgg
      121 taaggggtat tgcaggtgtt gagaacggtg atatgctcag cggaacagtc aaggtgacca
      181 tgaagaaggg aaggacccct tttaccgcca agataaaaac agaccccggc ataaagcagt
      241 tctatgccgg caagggtctc tcactgggag acaaggcggg gacactgaac ctggattttg
      301 actatacgca gtcttttgat gacatcaggg taaaatacaa gactttcaac aggcttaatg
      361 gcggtctgat ctataacaac aacctcttca ctgaaagcaa gccactcatc ctgagccttt
      421 ctgtacgtgg ttcacagacc ctggatatca gcaagagaga tcccgatatg cttgatattg
      481 agaattatga gtcaaaggat cagagtctcg ccatgaatct caatgccaaa tgggccatga
      541 actccgcggt actgacaaac cttaacctgg tggtctcagg tgacatacag caccaggtgg
      601 gtcatgaggt gaccatggag gagataccgt caggtgcaca gccgcaaccg gtggcttacg
      661 agccgggaga gaaccaggtg cccatactgc cctcctcata tatcagcgat ctcaccattg
      721 acggccgtcc ctattattac aatgcaaagc tttcaggcaa ccggtcattt aacatcggag
      781 cagccctcaa taacattgtt gcagggattg aatacagact cataggcaat aacggcctgg
      841 gaaggatata tgaccttgca cgtcctccgg ttccggtagg aggcacagtt gcaaggccca
      901 ggtcatacaa ggatatacca tctcttggtc agctggcata ttatgccgaa gagaacctgg
      961 gaatacccat aggcaagaca aggctggacc tgcaggctgg aatcaggttt accaatgtgc
     1021 agcctgatgg cctgttcaga tcgcgggagc acaccactat gcttgatccg aggttcaatg
     1081 cgagattcac cgtaattgac aggggtcagg gaatcatctc gaatcttgca ttgagagggg
     1141 gttatggtct cttttccaaa gctccgtcac tgctctatct ttatcctgac agggcctatt
     1201 gggacaagac gggtctaagc tattatgatc cgatagctca gagaggtttg ttcattgtga
     1261 ctaccaaaat ctttgaaaac ccacgtaacg aaaatctgaa tccggcagtg aaccgtaaaa
     1321 ttgaaggtgg gtttgacctg aaagttaagg atttgagcct gaatgttact ctttattcag
     1381 agaaaatgga taatggcttc agcttcgaat catactatga gaatttcatc ttcaacagat
     1441 atattcaacc gttgcagaat ggtctggatc tctatttcgt gcccggctcg ggagtattct
     1501 atattgatcc ggactctggc gatgaggtac aacttcccgt ggtacaagac actgtattcg
     1561 tcagctttac ttacccgggg aacgggacga tgacaacaaa aagaggtgtt gagtttactc
     1621 ttgacctggg aacggtaaat gccctgcaga catcattcat tgtggacggt gcctggatgt
     1681 gggtgaagag gcagaccaca gtgaatgatt tgctcaagcc atctacgggt agtataggcg
     1741 gcaaggaatt tcccatggtt gctgtctatc cggcagggta tggcagcatt gacaagcgat
     1801 tcaatacaaa catcaggact ataacccata tcaggaaatt gcgcatggtg gcatcggtga
     1861 ccacacagat aatctggttg gactgtgacc aaagcgtatg ggatgaccct gagggcaatc
     1921 cccttttcta tactgatcct gaagttcttg atccatatgc cgacaagctt ttccccaagt
     1981 atgttgatcc tttgggatat tatgaccggc agatggttta tcatgagttt gaccggtccc
     2041 tggcagtcac caaaccctat tctgacctga taaaaaggta caatacacca tggttctttg
     2101 ctgagacaga ctatccgcca tacttcgtga tcaatttcaa gctcaccaag gagattactg
     2161 acaaagccaa cttttcattc tatgcaaaca acattacaaa tcataatcca ctgctgaaac
     2221 agcgaggggg cgctcccgag gtctatggac gaaggaaccc tccactctat tttggcgctg
     2281 agataaagat taagttctaa ccttaaattc aatatcatga aaaaatgcac aatttttttg
     2341 gttgttcttc ttgccggaat ggcagcactt acctcatgca agaagggagc acaactctat
     2401 gatgtaacca tctgggctta ctatcccgag ggttacggta agaattatgc ctctgatgta
     2461 gacatcgtgg ttaagaatac aatctccgga caggagttta atctcgtgac cggccatcag
     2521 ggttttgttg aaatacaact cgaagaaggt atgtataaag tatcggctac caaggagaca
     2581 gacgatttta atcttaacgg aataaccgag aatcttactg tacagacgac cgggttaaat
     2641 aactgggagg ttcatctggt ggctacctca aaagcaggag gtcttgtgat caaggaaatc
     2701 tactatacag gctcaaagac cgaaagtggc aacaactatt attccgacca gtttcatgag
     2761 atatacaaca acagcgatga gcttgcctat cttgacggtc tttgtatagc tctcacctat
     2821 ccgataggat caactcctac cgagtttgtt aatgaggatg gttcaattat ggaccgtata
     2881 ccgtgtaatt tccaaaccct gatgttcccc ggcacgggtc agcaatatcc tctggaaccc
     2941 aggaccagtg tcgtgcttgc aatggatgcc attgaccaca agacagatcc ggtgggtaat
     3001 ccgctctctc ctgtcaacct tgccaatgca ccgtttgaag cgttcattga ggccgccggc
     3061 aaggacaccg atgcaccgca ggctaccaac atgattgtga tatacactac ctccccaaca
     3121 atgcatgact ggcttcattc agtcaatggc cagggagtga ttcttttcag attgccgaca
     3181 ggactcgact gggagacatt tgttgccaat cccgataatt tcatgaccaa gcccgggtcg
     3241 tcatcaccta tagaatattt aatggtccat aaagactggg ttattgacgg agttgaatgc
     3301 aaccgtacag aggaagataa aagattcaag aggctgccta cctcagttga tgcaggtatg
     3361 gtatggagtt tagccaccta caacagcaag agcgtgagac gtaaagttga gaagattctt
     3421 gacggaaagg tcatctacaa ggacaccaac aactcttaca acgactttat caacgatcag
     3481 gttccgacac cctttgttca tccaacaacc gttgacaact agctgatcaa ataaagcgag
     3541 cgtgccggac cgggggcaaa cagcctgtcc ggcaccccgc tttaaacaac tgaatattca
     3601 gggtaatgaa gaatctgaca gttttgcttt ttctgatatg tctgatcctg gcaggtaccg
     3661 gcagtacttt cgcggcggca gacaccactg cggtgccagc gggtagccga gcttttgacc
     3721 tcatgccatt tgt
//
LOCUS       DAOPBK010000210         3731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_19990_length_3731_cov_5.164853, whole genome shotgun sequence.
ACCESSION   DAOPBK010000210 DAOPBK010000000
VERSION     DAOPBK010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19990_length_3731_cov_5.164853"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2439
                     /locus_tag="PLA17_10135"
     CDS             <1..2439
                     /locus_tag="PLA17_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPK85811.1"
                     /translation="ANWDIVKETPEGGSGDQTVADATTGEDTEGEEPSDMKLMLRKFA
                     INDGRIGYTDHESDMQVAVNDLSFLLSGNLSGSQSVLDLVIDAGKVDFVMDKISWLTG
                     AAMSFRAGIDAVPDSMKFTLRDNVLKLNDIALNFSGTAAMPGDDIDLDMVFSAPETSF
                     KSLLSLIPAFYMKGYEDLRATGMVALDGAVKGIYSSADSTLPDVLLNLKVSDGLISYP
                     ALPEKISEINIAGKVQTDGNDMDKTIAEISRFHFVLAGNPFDLSLAMATPLSDPAVSA
                     AAKGKIDLGKLQQAIPLDSITLNGLLDISLDLAGRMSMIENKEYEKFRAVGDLKIADM
                     AVEMSGMPDMRISSAAFAFSPAFAEMTGMKASMGKGSDFSLSGRLENYIPYLFSDGIL
                     RGNLSLKSDHVDLNEIMVYIPSDTLETDTVPMEVITIPKNIDFTLEALVGKLNYGMLE
                     ASDVKGNVVVREGVVTVSETGMKALGGSLLVNALYDTRDTLKPLIDAEMFVSAVSIRD
                     AFNAFNIVKKLMPAASGLGGNVSARIDFRSILGTGMMPLLKTLSGTGEIASESVQVLN
                     STGFDRMKNVLKINPSYTNVLKDIRATFIINDGRLWIKPFDTMLGKIKLNLSGDQGLD
                     QTINYLVKVEIPREELGQAAGALMSTFASQSAALGFSGAPPEMIKVNLNVGGTVKNPA
                     FTPSFAGGGASSAVTALADTLKSEVVEKVNEAARQQAEKIMKEAEENANLIREKAASS
                     AETIRSEAHLRGKKLISDAEASGPIAVAAARKAAERLNREADKRATQIVDEANKRADE
                     VLATARSKAEDLLK"
     gene            2616..>3731
                     /locus_tag="PLA17_10140"
     CDS             2616..>3731
                     /locus_tag="PLA17_10140"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPK85812.1"
                     /translation="MKKQLILLLIFSILLPFNSFSQTPGGTITGVIREQSTGETMPGV
                     NVIIEGTLRGASTGPDGTFTIAGVPPGIYSLSLTFISYKPLKVEGVEVKAGSVTVIDA
                     AMYDQSVELDDIVVVATKRTDSEVSVMSSIRSSPVVVSGISAQLISRTQDKDASEVVR
                     RLPGVSIVDDRFIVVRGLSSRYNNVWLNNATAPSLEPDTRSFSFDLIPSSMIENIFIL
                     KSPTAELPADYSGGFIKISTTGIPDENRFSVSYGTALRQYTTFGDYLEERGGKTDWLT
                     LEAGHRELPEAMPVHLDLYESATNPEIRNRVTELGRSLNGSWDAAQATALPDQKLSLG
                     LTRRFEKGSFIAGNVTSLTYSYSNTFREALNNNYSIYN"
BASE COUNT         1006 a          866 c          976 g          883 t
ORIGIN      
        1 gccaattggg atattgtaaa ggaaactccg gaaggaggct ccggtgatca gaccgttgcc
       61 gacgccacaa ccggagaaga taccgaaggc gaggaacctt ccgacatgaa gctgatgctt
      121 cgaaaatttg cgattaacga cggacgaata ggttacactg accatgagtc agatatgcag
      181 gttgccgtta atgatctcag tttcctgctt tcaggaaacc tgagcggttc acagagcgtg
      241 cttgatcttg tgattgatgc cgggaaggtt gattttgtca tggacaagat ttcgtggctg
      301 accggtgctg ccatgagttt cagggccggg atagatgcag ttcctgactc aatgaaattt
      361 actctcagag acaatgtctt aaagctaaat gatatagcgt tgaacttctc cggcactgcc
      421 gcaatgcccg gagatgatat tgatctcgac atggtattca gtgcacccga gacctccttt
      481 aagtcgctcc tctcactgat accggcattt tacatgaagg ggtatgagga tctgagggct
      541 accggaatgg tggcgctgga cggcgccgtc aagggtatct acagttcagc cgacagcacc
      601 cttccggatg ttttactgaa cctgaaggtc agtgacgggt tgataagcta ccctgcactt
      661 cctgaaaaga tcagcgaaat aaatatcgcc gggaaagtgc agacggacgg aaacgacatg
      721 gacaaaacca tagctgagat cagccgtttc catttcgtgc tggccggcaa tccttttgac
      781 ctcagccttg ccatggccac tccgttgagt gacccggcag tcagtgccgc ggcaaaggga
      841 aagattgacc ttggaaaact tcagcaggcc attccccttg acagcatcac cctgaacggc
      901 ctgctggata tctctcttga tcttgcggga cgaatgtcaa tgattgagaa caaagaatat
      961 gaaaaattca gggcagttgg tgacctgaag atagctgaca tggcggtaga gatgtcaggg
     1021 atgcctgata tgagaatcag cagtgcagcg tttgctttca gcccggcatt tgcggaaatg
     1081 acaggcatga aggcctcaat gggcaagggc agcgattttt ccctgtcggg caggctggag
     1141 aattatatcc catacctctt ctcggacgga attctccggg gaaacctgtc gcttaaatct
     1201 gaccatgttg acctgaatga gataatggta tatattcctt ctgacactct tgagactgat
     1261 accgtcccga tggaggtgat aacaataccc aaaaacattg actttaccct tgaagccctg
     1321 gttggcaagc tgaactacgg gatgctcgag gcaagcgatg tgaaaggcaa cgttgttgtc
     1381 cgtgagggtg ttgtaactgt cagcgagacc ggcatgaaag cacttggagg ttccctgctc
     1441 gtaaatgcac tgtatgatac acgtgatacg ctcaaacccc ttattgatgc tgaaatgttt
     1501 gtcagcgcgg taagtataag ggatgccttc aatgccttca atattgtgaa aaagctcatg
     1561 cctgcagcgt caggactggg aggcaatgtc tcggccagga ttgacttcag gagcatcctc
     1621 ggaacgggca tgatgccact gctgaaaacc ctttcaggga caggtgagat agcctctgag
     1681 tcagtgcagg tattaaattc aacaggtttt gacaggatga agaacgtgtt gaagattaat
     1741 ccttcctata ccaatgtgct gaaggatatc cgggctacct tcatcatcaa tgacgggagg
     1801 ctgtggatca agccttttga cacgatgctc gggaagataa agctgaattt gtcaggtgac
     1861 cagggccttg atcagacaat aaactatctt gtaaaggtgg aaataccaag ggaggagctg
     1921 ggtcaggctg caggggcact gatgagcact tttgcttcac agtcagctgc cctgggtttc
     1981 agtggcgcac cacctgaaat gattaaggtt aacctgaatg ttgggggtac agtaaagaat
     2041 ccggcattca caccctcttt cgccggcggc ggcgcctcat ctgccgtgac tgccctggct
     2101 gataccctga agagcgaggt ggtggaaaag gtcaacgagg cagcaagaca gcaggcagag
     2161 aagatcatga aggaggctga ggagaatgca aatctgataa gggagaaagc tgcatcatcg
     2221 gctgaaacga tacgcagtga agcccatctg cggggtaaaa agctcatcag tgatgcggaa
     2281 gcaagtggcc cgattgccgt ggctgctgca agaaaagcag cagaaagact gaacagggag
     2341 gctgataaaa gagccacaca gattgttgat gaagccaaca agagagctga cgaggttctt
     2401 gccactgcca ggtcaaaggc tgaggatctg ctcaaataac ctgttacaga agaggctaag
     2461 acatttaaaa gaggttgaaa tgacctcttt ttttttgcta tcaagtcagc ctgtcacaat
     2521 tatgtaacat aagtgtcatc tattcgttac cggtttttta cactcctgta acatcagcgg
     2581 ctgacttttg ccgtgtattt aagtcaacca tcaaaatgaa aaaacagtta attcttttat
     2641 tgatcttttc aattcttctt cccttcaatt cattttcaca gaccccgggc ggcaccataa
     2701 ccggcgtaat tcgcgaacag tctaccggag aaaccatgcc cggggtcaat gtgataatag
     2761 aagggacgct caggggcgca tcaaccggac ctgacggaac atttaccatt gccggcgtac
     2821 cccccggaat ctacagcctg agtttaacct ttatatcata caagccactg aaagtggaag
     2881 gtgttgaggt caaagcaggt tctgttacgg tcatcgatgc tgccatgtat gaccagtctg
     2941 tggaactgga tgatattgtg gttgtcgcta ccaaacgtac tgacagcgaa gtgtcggtaa
     3001 tgagcagcat caggagcagc ccggtggtcg tttcaggtat ctctgcccag ctgatcagcc
     3061 gtacgcagga taaggatgct tcggaggtgg taaggagact tcccggagta tccattgtgg
     3121 atgacagatt catcgttgtc aggggactct catcaagata caacaatgtc tggctcaata
     3181 atgcaacggc tcccagcctc gagcctgata caaggtcctt ctcgtttgac ctgattccct
     3241 catcaatgat cgagaatatt ttcatcctga agtcacccac ggctgaactc ccggcagact
     3301 actccggcgg gttcatcaag atatcaacaa caggcattcc tgatgagaac aggttttctg
     3361 tttcatacgg tactgcactc agacaataca ctacattcgg cgattatctc gaggagagag
     3421 gcggtaaaac agactggctc accctggagg ctggccacag ggaactgccg gaagcaatgc
     3481 cggttcatct tgacctttat gagagtgcca ccaatcctga aatacgcaac cgggtcaccg
     3541 aactgggacg aagtctcaac ggctcatggg atgcggctca ggcaacagcc ctgccggatc
     3601 agaaactttc tctgggtctc acaagacggt ttgaaaaggg aagcttcatc gccggtaatg
     3661 tcacatcact gacctacagc tattcaaaca ccttcaggga ggcgttgaac aataattact
     3721 caatatataa t
//
LOCUS       DAOPBK010000211         3722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_20053_length_3722_cov_4.360785, whole genome shotgun sequence.
ACCESSION   DAOPBK010000211 DAOPBK010000000
VERSION     DAOPBK010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20053_length_3722_cov_4.360785"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLA17_10145"
     CDS             complement(<1..564)
                     /locus_tag="PLA17_10145"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPK85813.1"
                     /translation="MIKPVKTKLLVAALLMALAAGTAVSQELYCNVQVSAQKIQGSNR
                     EVFQNMQRDIYEFMNSMVWTDNIFSFSERIECNLLINLDEQLSADEFRGTVQIQLSRP
                     VYNTTYKSTVLNFVDNNFQFRYVEFQPLEFNPNSHTSNLVSVLAYYAYLLIGMDFDTY
                     SQGGGTPYFQVAEKIVINAQNAPEQGWK"
     gene            complement(571..1782)
                     /gene="coaBC"
                     /locus_tag="PLA17_10150"
     CDS             complement(571..1782)
                     /gene="coaBC"
                     /locus_tag="PLA17_10150"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPK85814.1"
                     /translation="MLEGRKILLGVTGGIAAYKAAVIIRLLVKEGAEVKVVMTPLAKE
                     FITPLTLATLSRNPVISDFFDPEDGRWNSHISLGLWADLYLIAPATANSIGKMAAGIA
                     DNLLLTTYLSARCPVFLAPAMDMDMLAHLATISNIERLKSFGNHIIEPDTGELASGLD
                     GKGRMAEPEVIVRELREFLSKKKTGEQPLKGRRVLINAGPTVEPIDPVRYISNYSSGR
                     MGIALAEAAAAMGADVTLVLGPVSIKPRSGAITIVPVTTAAEMKEATVAAFSTCDIAI
                     LAAAVADFTPEHRHETKVKRGDDDMVIRLKPTDDIAACLGKMKRKGQLLAGFALETDN
                     ELENAKGKLRRKNLDLIILNSLKDQGAGFGHHTNRVTIIDSNDNIDKFELKTKDEVAL
                     DILKKIISLIR"
     gene            complement(1791..2126)
                     /locus_tag="PLA17_10155"
     CDS             complement(1791..2126)
                     /locus_tag="PLA17_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPK85815.1"
                     /translation="MDYKKINAPLTTITRDIDKFDAQTGNIYESVIICSRRANQISVE
                     MKQELNSKLEEFASFNDNLEEVFENREQIEISKFYERLPKPTLIALSEFEEDKIYYRN
                     PDKEQKQRK"
     gene            complement(2143..2961)
                     /gene="bamD"
                     /locus_tag="PLA17_10160"
     CDS             complement(2143..2961)
                     /gene="bamD"
                     /locus_tag="PLA17_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPK85816.1"
                     /translation="MRIRPIIFFMLALVITSCGEYEKLLKSTDYELKRQKIFDYYDDG
                     KYIRSIELLSQILPRYRATDHAEQLNWINAQAHFRIRDYMMAGRFYQEFADTYPGSNN
                     AEEASYLSALCDYYQSPRPELDQANTRKAIESFTIFMQRYPTSTHNDECKAKILELQE
                     KLVEKSYLSARLYYDLKEYRAATVSLANSLKEYPETKYREELMFLKLDALYLLAVNSV
                     PEKKVERYQTTLDEYYSFIEEYPQSKFTKDVNRIFESTTKYLKVDTAVKQTENN"
     gene            3153..>3722
                     /locus_tag="PLA17_10165"
     CDS             3153..>3722
                     /locus_tag="PLA17_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK85817.1"
                     /translation="MDIFDKIRKNRGALGQYQEQADGYFMFPRLEGEIGPRMKFMGKE
                     VLNWSLNNYLGLANHPEIRKVDAEAAAQYGLAYPMGARMMSGQTTMHEKFEAMAAEFV
                     GKESAYLLNFGYQGMVSIIDSLVGRHDVIVYDSESHACIMDGLRMVLAKRFVYAHNDM
                     ESFEKQLQRAQKIVAETGGGILVITEGVFG"
BASE COUNT          888 a          907 c          885 g         1042 t
ORIGIN      
        1 tttccagccc tgttcagggg cattctgggc attgatgaca atcttttcgg caacctggaa
       61 atagggggtt ccgcctccct gtgaataggt atcaaaatcc atcccgatca gaagataggc
      121 gtagtatgcc agcaccgaca caaggttgct cgtgtggctg ttcggattga actccagggg
      181 ctggaactcc acatacctga attgaaaatt gttatccacg aagttgagca cggtagactt
      241 ataagtggtg ttgtaaaccg gcctgctgag ctgtatctgt acagtgccgc ggaactcatc
      301 tgccgaaagc tgctcatcaa ggttgatcag caggttgcat tcgatcctct ctgaaaagct
      361 gaagatattg tcagtccaga ccatgctgtt cataaattcg taaatatcac gctgcatgtt
      421 ctgaaaaacc tcacggttcg aaccctggat tttctgagcc gacacctgca cgttgcagta
      481 cagctcctgc gacactgccg ttccggcagc cagtgccatc agcagtgccg ccacgagaag
      541 ttttgttttt accggtttga tcataagcct tcagcgaatc agtgaaatga ttttcttcag
      601 tatgtccaaa gctacttcgt ccttcgtttt cagttcaaat ttatcaatat tatcgttact
      661 gtcaatgatg gttaccctgt tggtatggtg gccaaatccg gctccctgat ccttcagtga
      721 gttgaggatt atcaggtcaa ggtttttcct gcggagctta ccctttgcgt tctccagttc
      781 attgtccgtc tccagcgcaa atccggccag caactggccc tttctcttca tcttcccgag
      841 gcatgcggcg atgtcatcgg tgggtttcag cctgatgacc atatcatcat cacctctttt
      901 tacctttgtt tcatggcggt gctccggggt gaaatcggct acggcggcag caaggatggc
      961 aatatcgcag gtgctgaatg ctgccacagt tgcttccttc atctctgccg cagtggttac
     1021 gggaacaatg gtgatggctc cggagcgggg cttaatactg acaggtccca ggaccagcgt
     1081 tacatctgca cccatcgcgg cagctgcctc ggcaagcgca atacccatgc ggcctgaaga
     1141 ataattgctg atatacctga ccggatcaat cggttcaacg gtaggccccg cattgatgag
     1201 aaccctcctg cccttcagcg gctgttcacc tgtttttttt tttgagagga actccctgag
     1261 ttctcttact attacctccg gctcggccat acggcccttg ccatcgagtc cgctggcaag
     1321 ctcaccggta tcaggctcaa tgatgtgatt gccaaatgac ttcagcctct caatattact
     1381 tattgttgcc agatgtgcca gcatatccat gtccattgcc ggggcaagga agaccggaca
     1441 ccgtgccgag agatatgtgg tcaggagcag attgtctgca atgcctgcgg ccatctttcc
     1501 aatgctgttg gcagttgccg gagcaataag ataaagatcg gcccagagac ccaggctgat
     1561 atgactgttc catcgtccgt cttccggatc gaagaaatcc gatataaccg gattccttga
     1621 aagggtggcc agggtcagcg gtgtgatgaa ctcctttgcc agcggggtca tcacaacctt
     1681 tacctcagca ccttccttaa caagcaaccg gatgatgaca gcagctttgt aggctgctat
     1741 acctccggtg actccaagca gtattttcct gccttccagc atgttgccag ttatttcctc
     1801 tgcttctgct ccttgtcagg attgcggtaa tatatcttat cttcctcaaa ctcggaaaga
     1861 gcgataagtg taggcttcgg cagcctctcg tagaactttg atatctctat ctgttccctg
     1921 ttctcgaaaa cctcttccag attgtcattg aatgaagcaa actcttccag ctttgagttc
     1981 agttcctgct tcatctcaac cgatatctga tttgcccttc tgctgcagat aatgacgctt
     2041 tcgtaaatat tccccgtctg ggcatcgaat ttgtcaatat ccctcgtaat ggttgtcagg
     2101 ggcgcattta tctttttgta atccattttt atattgatga tttcagttgt tttcagtctg
     2161 tttcacggct gtatccacct tcaggtactt tgtcgtgctt tcaaatatcc tgttcacatc
     2221 cttcgtaaat ttgctctgag ggtactcttc tataaacgaa taatactcgt caagtgttgt
     2281 ctggtagcgc tcaacctttt tctcgggaac actgttcacg gcaaggaggt agagggcatc
     2341 aagcttgaga aacataagct cctcccggta ttttgtctca ggatactcct tcaggctgtt
     2401 tgccagtgat actgttgctg cgcggtattc cttaaggtca tagtagagcc tggcgctcag
     2461 gtatgatttc tcgaccagct tttcctgaag ctcaagaatt tttgccttgc attcatcatt
     2521 atgggtgctt gtcggatatc gctgcatgaa tatcgtaaag ctctcaattg ccttcctggt
     2581 gtttgcctga tcaagctcag gcctgggact ctggtaataa tcgcacaggg ctgacaggta
     2641 ggaagcttcc tcggcattgt tgctgccagg ataggtgtcg gcaaactcct ggtaaaacct
     2701 ccctgccatc atatagtcgc gaatcctgaa atgtgcctgg gcattgatcc agttgagctg
     2761 ctcagcatga tcggtagccc tgtatctggg cagaatctgt gagagaagct caatagacct
     2821 gatgtatttg ccatcatcat aatagtcaaa tatcttttgt cttttgagct cgtagtcagt
     2881 actcttgagg agcttctcat actcaccgca tgaagtgatg acgagagcaa gcataaagaa
     2941 tattatcggt ctgatcctca tacccggaaa tgttgcgcaa aattactctt ttttttcatt
     3001 gattgaccta aatatactac aaaagtgcac caataattga aacagagtag ttaagagagt
     3061 gttaattccg cgaaacaaat aggtataaat aggtactttg acggtgcaat gtttttgata
     3121 attttgcgtg caataatcaa aactatactg acgtggatat tttcgacaag atcagaaaga
     3181 accgtggtgc gctcggacag taccaggaac aggcagacgg atattttatg ttccccaggc
     3241 ttgaagggga gatagggccc cgcatgaaat tcatgggcaa ggaggtgctt aactggagtc
     3301 tcaacaacta tctggggctc gccaatcatc ctgagatacg aaaggttgac gccgaggctg
     3361 cagcacagta cggactggca tacccgatgg gtgccaggat gatgtcaggc cagacaacca
     3421 tgcacgaaaa atttgaggca atggctgccg aatttgtcgg aaaggaatcc gcatacctgc
     3481 tcaacttcgg ttaccagggg atggtctcca taatagacag ccttgtcggc aggcatgatg
     3541 tcatagtgta tgattccgaa tcacatgcct gcatcatgga cggactgaga atggttctcg
     3601 ccaaaaggtt tgtgtacgct cacaatgaca tggaaagctt cgaaaagcag ctccagcgtg
     3661 cacagaaaat cgttgcagaa accggaggag gaatcctggt catcactgag ggtgtcttcg
     3721 gc
//
LOCUS       DAOPBK010000212         3719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_20066_length_3719_cov_4.033570, whole genome shotgun sequence.
ACCESSION   DAOPBK010000212 DAOPBK010000000
VERSION     DAOPBK010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20066_length_3719_cov_4.033570"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PLA17_10170"
     CDS             complement(<1..1168)
                     /locus_tag="PLA17_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPK85818.1"
                     /translation="MKKQNWYKLLYQFAIIGILTFMGFRLLFDKAYAPDFEAYCPFGG
                     LQALGSYLSLGSLSCSMTSTQIMMGVVLFIGIVLFSRLFCGYICPLGTVGEWIGKLGD
                     RLKVRITPKGFIDCALRLLKYVLLFITFYFTLKSSELFCKKFDPYYAVTSGFDTDVVL
                     LWALMAIVALVVGSLFVRLFWCRYLCPLGALSVIFRFSWWFLGVMALYVILLLAGLEI
                     SYVYPLLVITAGGYILEVTRMKRVRPYPIHITRNTETCIDCGLCSETCPQGIDVARME
                     KVTHTDCTLCGDCLHACPEKDTLQINKKSMKWLPAAVLGALIILGIVAGTLFELPTID
                     QKWGTPEQIESAGIFEMEGLKNIKCFGSSTAFANQMRRHEGVYGVATYVASHKVR"
     gene            complement(1287..1619)
                     /locus_tag="PLA17_10175"
     CDS             complement(1287..1619)
                     /locus_tag="PLA17_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85819.1"
                     /translation="MPDFFSDLAGNMKGKKIDVPDSFADNVISVIERRKEGGFKSMSM
                     ASRIFISLVVLVIYCSLGILLGVKGYSDMQPDDSSSSQEALVELMDSHYISTDLLHDP
                     FFRNFVCR"
     gene            complement(1695..2219)
                     /locus_tag="PLA17_10180"
     CDS             complement(1695..2219)
                     /locus_tag="PLA17_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85820.1"
                     /translation="MISEKTNRILLWFNLILILIITSAVITFLVMKRSGEKQTNDPGV
                     INSMDMLRKELNLTDEQYRTVLLENDRTIRKYNIVFDMMCETNVAMLEELAKEVSDPA
                     VLDSLSRKVGTLSVSLRRHTTDYFMNLKSICNEDQQKELTEILKRMMQIDELCKSCNK
                     RECPRKERINNIGK"
     gene            complement(2231..2821)
                     /locus_tag="PLA17_10185"
     CDS             complement(2231..2821)
                     /locus_tag="PLA17_10185"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK85821.1"
                     /translation="MSDNELIKAVIRGDNSAMRQLISKYQSLVLNTCYKVLQSRQDAE
                     DISQEVFIEVYRSAAGLRYEENISFWLYRISLNKSINHLRRSQNILFRSLMKIETLFR
                     HDDAGSMSPEPHSDDHPDESLEAAERLEIVKKAVATLPANQQKAFILYYYEELSYKEI
                     GSVLKLSVSSVESLLFRARENVKKRCCPEKPVKKAI"
     gene            complement(2886..3323)
                     /locus_tag="PLA17_10190"
     CDS             complement(2886..3323)
                     /locus_tag="PLA17_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85822.1"
                     /translation="MPKLISLFSLLIILSLATSCTNSSGNQKVTASETPAGITADAKL
                     NSDLNAKLGSWLKKDVECYGIVVGYFADGSTLGKSVKCRVVAIKPDKIKMKTIESVSL
                     LPGEGCDKMGLSYGDTWWEEDGDIFRTREEAEAYIRDKGWAAK"
     gene            3420..>3719
                     /locus_tag="PLA17_10195"
     CDS             3420..>3719
                     /locus_tag="PLA17_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85823.1"
                     /translation="MQKKQRPAVECRCTFTTGGVVTIDSPRGGVSLHSHDRRRCESCF
                     APKGQYPDQQTRMSPGREPGTAIPDAAASDCLFYRPDECPDAHLLVDVINAPYRLQ"
BASE COUNT          959 a          922 c          838 g         1000 t
ORIGIN      
        1 cccgcacctt atggctggca acataggtgg cgacaccgta gaccccttcg tgcctgcgca
       61 tctggttggc gaaagccgtt gagctgccga agcacttgat attcttaagc ccttccatct
      121 caaatatgcc ggcgctttca atctgttcag gcgtgcccca cttctggtca atggtaggca
      181 gttcgaacaa agtacctgcg acgatgccca ggatgataag cgctcccagc actgctgccg
      241 gaagccactt catactcttt ttgttgatct ggagagtatc cttttcagga catgcatgga
      301 gacagtcgcc gcacagtgtg cagtcagtat gagtcacctt ctccatccgt gcaacgtcaa
      361 tgccctgggg gcaggtctcc gaacacagcc cgcaatcaat gcacgtttcc gtgttgcgtg
      421 tgatatgtat gggataaggc cttactctct tcatccttgt cacctcgaga atatatcctc
      481 ccgccgtgat tacaagaagc ggatatacat atgatatttc aagacccgcc agaagcagta
      541 tcacatagag tgccatcacg cccaggaacc accatgagaa cctgaatatg acagacaggg
      601 ctcccagagg gcacaggtac cggcaccaga agagtctcac aaaaagtgaa ccgaccacaa
      661 gcgcaacgat ggccatgaga gcccacagaa gtacaacatc agtgtcaaaa ccggaggtaa
      721 cggcatagta agggtcaaat ttcttacaga acagttcgct cgatttcagg gtgaagtaga
      781 atgtaataaa cagcaataca tactttagca ggcggagggc gcagtcaata aaacccttcg
      841 gggttattct caccttcagc ctgtcaccca gttttcctat ccactcaccc acggttccga
      901 gaggacatat gtatccacag aagagcctgg aaaagagcac aatgccaatg aagagaacca
      961 ctcccatcat gatctgtgtg ctggtcatcg aacatgagag agagcccagg ctgaggtagc
     1021 tgcccagtgc ctgaaggccg ccaaacggac agtaagcttc gaagtcggga gcataagcct
     1081 tgtcaaagag caggcggaag cccataaatg tgagtatacc aattattgca aattgatata
     1141 ggagcttgta ccagttttgt tttttcattt gtgatatctg ttattgtttc tttgatctaa
     1201 tatgacttac aagatattta tgtttgatct ctgttgtgaa ctgttattcg tgcaaggctc
     1261 attggaatgg tgtgcagatt attaaattac cggcagacaa aatttctgaa gaatggatca
     1321 tgcaggaggt ctgtactgat atagtgtgag tccatgagtt ccaccagtgc ctcttgtgaa
     1381 gatgagctat catcaggctg catgtcactg tagcccttca ctcccagcag tatacccagt
     1441 gaacagtaga ttaccagcac aaccagggaa ataaatatcc gcgaagccat tgacatggat
     1501 ttgaaaccac cctccttcct tcgttcaatc actgatatga cattgtcagc gaatgagtcg
     1561 ggaacatcta ttttcttgcc cttcatgttt ccggcaagat ctgagaaaaa atctggcatg
     1621 attgttcaga ataaaaataa tcgtttacta aaaagacaag ttggaagcct gaaacctgcg
     1681 gtcatagtat aaaattattt tccgatgttg ttgatccgct ctttccttgg acattctctc
     1741 ttgttgcagc tcttgcacag ttcatcaatc tgcatcattc tctttaagat ttcggtaagc
     1801 tctttttgct gatcctcgtt gcagatgctt ttcaggttca tgaagtagtc agttgtatgt
     1861 ctcctgagtg agacacttag tgttcccacc ttcctgctga ggctgtcaag caccgccggg
     1921 tcactgacct cctttgccag ttcttcaagc atggcaacgt tggtttcgca catcatgtcg
     1981 aagacaatgt tgtatttgcg gattgtgcgg tcattttcca acagtaccgt ccggtattgc
     2041 tcatcagtca ggttgagctc ctttcgtagc atgtccatgg aattaatcac tccggggtca
     2101 ttggtttgct tctcacctga tcttttcatt accaggaaag taattacggc cgatgtgatg
     2161 atcaggatca ggatcaggtt gaaccaaagc aaaatcctgt tggttttttc acttatcatg
     2221 atttgaagtt ttaaatagct ttttttacag gtttttccgg acagcatctt ttctttacat
     2281 tttcacgtgc acggaaaaga agtgactcaa ctgaagagac cgacaatttc agcacgctgc
     2341 ctatctcttt atatgaaagc tcctcatagt aatacagaat aaaggctttc tgctggtttg
     2401 ccggcagggt tgccacggcc tttttaacaa tctccaatct ctcagccgcc tccaggcttt
     2461 cgtccggatg atcatcagaa tgcggttccg ggctcatgct cccggcatca tcatgacgga
     2521 agagagtctc tatcttcatc agtgaacgaa acagtatgtt ctgtgatcgc ctgaggtgat
     2581 tgattgactt gttgagtgaa atgcggtaca accagaatga gatgttctcc tcgtatctca
     2641 gcccggcagc agatcgatat acttcaataa atacttcctg ggaaatatcc tctgcatctt
     2701 gccgcgactg caagaccttg taacaggtgt tcagcaccag gctctgatat tttgatatca
     2761 gttgacgcat ggcgctgtta tcacccctga taacagcctt tatcagttca ttatcactca
     2821 tccctgacgg cagagagata gaaaagcggg ataatcacca acatggcata acgcttcccg
     2881 cgaggctact ttgcagccca gcccttatcc ctgatatagg cttctgcctc ttccctggta
     2941 cggaagatgt ctccatcctc ctcccaccag gtgtcgccat aggacaggcc catcttatca
     3001 catccctcac ccggcaacag actgacgctc tcaattgtct tcatcttgat cttgtcaggc
     3061 ttgatggcaa caacacggca tttaacagac ttgcccagcg tgctgccgtc agcaaaatac
     3121 cccactacaa taccgtagca ttcgacatct tttttcagcc acgatccgag tttggcgtta
     3181 aggtcagaat taagctttgc atctgctgtt attcctgcag gagtttcaga agctgtaacc
     3241 ttctgatttc cacttgaatt tgtacatgat gtggcaagtg acaggatgat gagcagtgaa
     3301 aacagagaaa tgagttttgg catggtttac agttttgttt tggtaattat ttcgacataa
     3361 aattagaaat tattcctttt ataaataaaa acacttgaaa catagcgcag gaaactggca
     3421 tgcagaagaa acaacgcccc gcggtggagt gtcgctgcac cttcaccaca gggggcgtgg
     3481 tgacaattga ttcaccccgc ggtggagtgt cgctgcactc tcacgacagg aggcgttgtg
     3541 agagctgctt cgccccgaag gggcaatacc cggatcagca gacccggatg tcaccaggga
     3601 gagagccggg aacagccatt ccggatgccg ctgcgtcaga ctgtctcttc tatcgaccag
     3661 acgaatgccc tgatgcccac ctcctggttg atgtcatcaa tgcgccgtac cgcctccag
//
LOCUS       DAOPBK010000213         3690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_20274_length_3690_cov_3.524347, whole genome shotgun sequence.
ACCESSION   DAOPBK010000213 DAOPBK010000000
VERSION     DAOPBK010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20274_length_3690_cov_3.524347"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1061)
                     /locus_tag="PLA17_10200"
     CDS             complement(60..1061)
                     /locus_tag="PLA17_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPK85824.1"
                     /translation="MSKKLAVIALGGNALLRGNQVGTIEEQEQNTTDTLENLIFLLRE
                     GYDLVITHGNGPQVGNILMQNDAGEQMYGIPQMPLDICVADTEGGVGYMIERMFRNVL
                     NRHGINKNVVCLITTVVVDKDDPAFNDPQKRVGEIYTREHAEELAKAKGWIFKEEVKA
                     HGGFRRVVPSPRPMCIMNHDLIGELARKGNIVIASGGGGVPVYIDENNEIRRAEVVID
                     KDLASSLLASKIGADEFYILTDIPYVYINYKKPDQEVKEFLDYKDAMKYLNEGQFAKG
                     SMAPKIEACLNFVKSGGRQCVITEAFQLEDRSYGTRITMEYEQATGSSDENSSHYGS"
     gene            1222..1911
                     /locus_tag="PLA17_10205"
     CDS             1222..1911
                     /locus_tag="PLA17_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85825.1"
                     /translation="MKYNPLFLITVILSSLLLASCKGDNKYRNMREGEIYYNVRYVKN
                     PSSISSDLLPKELVIAFRNDLISTRLKAPVGNIGVTTVVNPKENIYDTYLNILSFKYY
                     FPGNYRDMQPGFGSMEGITVHDTGRKTLICGFNCRQARVEMPDKKASRYIWYTTEIKA
                     ENPNRMTPYREVDGVLMDFFYIIGDAELQFTADEVLAKQIHDKEFQRKNNYRKVSAKY
                     LDTLIMKMISF"
     gene            1999..>3690
                     /locus_tag="PLA17_10210"
     CDS             1999..>3690
                     /locus_tag="PLA17_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPK85826.1"
                     /translation="MGKKKRGNSSSSQKRTSAGNQSGENLRFIVGLVLLVFALYLLVV
                     FISYVLTGNADQDSLSMQPDDKSFAYRNWGGKVGFRLGYYFMFNGFGLGSFFFPLVIG
                     AFGLALLRVKYFRLWKNVLRFLLAAVLVSVILGYFAGQSTFLGAGSPGGKFGFMIANY
                     LNDGLGKVGTGALLLVVAIAYLIFALHVSPQTIRQPIATVAKAGASMLNANGRKKDVV
                     TDKVTIAPEENYPSVEQEPLRADEEETEEIDDFYKPPVVSVENKPIVNVIDRNKKPVT
                     PEPFGEDSVNDLPPFDPKLSLSNYKFPTLALLRDYRSPGATTNDEVNANKEIILKTLA
                     DHKITIRQISATVGPTVTLYEVVPDRGVKIHRIKSLDNDIALSLSALGIRIIAPIPGR
                     GTVGIEVPNKKPETVSMKGLIASREFIDTKFELPLAMGRTITNDPYIIDLTKMPHLLV
                     AGATGQGKSVGLNAIITSLLYKKHPAELKLVLIDPKRVELPLYAKIERHFLAKLPDEE
                     DAIVTDTRKVINTLNSLCILMDHRLELLKLSQTRNIKEYNDKFIERRLNPEKGHDF"
BASE COUNT          970 a          948 c          822 g          950 t
ORIGIN      
        1 ttttataatg ctttactaca tgggcaaaat cgatgcccac aaatcacata tctgcaatgt
       61 tatgagccat aatggctgct attctcatca ctactgcctg ttgcctgttc atactccatt
      121 gttatcctgg tcccatagct cctgtcttcc agctggaagg cctcagtgat gacgcattgt
      181 ctgccacccg acttcacaaa attcagacag gcttcaatct tcggtgccat gctgcccttg
      241 gcaaattgcc cctcattgag gtacttcatt gcatccttgt aatcaaggaa ctccttgacc
      301 tcctggtcag gcttcttgta gttgatgtat acatacggaa tatctgtcag aatgtaaaac
      361 tcgtccgcac caatcttcga tgcaagcagt gatgatgcaa ggtccttgtc aatcactacc
      421 tcagcccgcc tgatctcgtt gttttcatca atatataccg gcactccgcc tccgccagaa
      481 gctatgacta tgtttccctt ccgtgcaagc tctccgatca gatcatggtt cattatgcac
      541 atcggcctgg gtgagggaac caccctgcgg aacccgccat gggccttgac ctcttccttg
      601 aaaatccagc ccttcgcttt tgcaagttcc tctgcatgct ccctggtgta aatctcgcct
      661 accctcttct gcggatcatt gaaggcaggg tcatccttgt caaccaccac cgtggtgata
      721 agacatacca cattcttgtt tatgccgtgc ctgttcagca cgttcctgaa catccgctcg
      781 atcatatatc ctaccccgcc ctcggtatca gcaacacata tatcgagggg catctggggg
      841 ataccgtaca tctgctcacc agcatcgttc tgcatcagta tgtttcccac ctgggggcca
      901 ttcccgtgtg ttattaccag gtcatatccc tccctcagga gaaatatcag gttctccagt
      961 gtatcagtgg tgttctgctc ctgttcttca atggttccca cctgattgcc tctgagtagc
     1021 gcattccctc ccagggctat aaccgcaagt tttttgctca tatattttaa tcgacattga
     1081 ggtagcaaag ctaaaatttt aaatgaaatt taaacatgcc aaatattatt ccggccatat
     1141 ctcagttatt ataataccac gaggccggtc gtggaggttg agtttttcat ttttactact
     1201 tttacagcca aatagtatag cgtgaagtac aaccccctct tcctgatcac ggttatcttg
     1261 tcctccttat tgcttgcctc atgcaagggc gacaataagt acaggaacat gagagaggga
     1321 gagatatatt ataatgtcag gtatgtcaaa aacccatcct ctatctcctc agatctcctc
     1381 ccgaaggaac tggtgatagc tttcaggaat gacctgatca gcacaagact caaagctccg
     1441 gttggcaaca ttggagtaac aacggtggtc aacccgaaag aaaacattta cgatacatac
     1501 cttaacatcc tgtcattcaa gtactacttt cctggaaact acagggatat gcaacccggt
     1561 ttcggttcaa tggagggtat aactgtccat gataccggga ggaaaacatt gatctgtggc
     1621 tttaactgca gacaggccag ggtggagatg cctgataaaa aagcatcgag atatatctgg
     1681 tacacaaccg aaataaaagc ggagaatcct aacaggatga ccccatacag agaggttgac
     1741 ggtgtgctta tggatttctt ctatattatt ggcgacgccg aactgcagtt tacggctgat
     1801 gaggtgctgg caaagcagat ccatgacaag gaattccaga ggaaaaacaa ctatcgcaag
     1861 gtctctgcaa agtacctcga tacacttatt atgaaaatga tttcatttta aagagactct
     1921 ttgcgtcagt caattttctt acctttgcta ttcgttgaac agaccgccgt ctcaaggacg
     1981 caataatttc atcgtacgat gggcaagaag aaaagaggaa acagttcatc atcacagaaa
     2041 agaacttctg ccggcaatca gtcaggggaa aatctgaggt tcatagtagg actggtgctc
     2101 ctggtttttg cactctacct cctggtggta tttatttcgt atgtacttac gggcaatgca
     2161 gaccaggatt cactcagcat gcagcctgac gacaaatcct ttgcctacag gaactgggga
     2221 gggaaggtcg gcttcaggct gggatattac tttatgttca acggtttcgg acttggttcc
     2281 ttcttctttc cgcttgtcat cggcgcattc ggactggcac ttctgagagt aaagtacttc
     2341 agactctgga agaatgtact tcgtttcctt ctggcagcag ttctcgtttc agtcatcctt
     2401 ggctactttg caggacagtc aaccttcctc ggcgccggat cacctggcgg aaaatttggc
     2461 tttatgatcg ccaactacct gaatgacgga ctggggaaag tagggacagg tgcactattg
     2521 ctggttgttg caattgccta cctcatcttt gccctgcatg tcagccccca gactatcaga
     2581 cagcctatcg ctacggttgc aaaggcaggc gcttcaatgc ttaacgccaa tgggaggaag
     2641 aaagatgtgg taacggataa agtcacgatt gcccccgaag agaattaccc gtctgttgaa
     2701 caggaacccc tcagggcaga tgaggaggag actgaggaga ttgatgactt ctacaaaccg
     2761 ccggtggttt ctgttgaaaa caaacctatt gtgaatgtaa ttgaccggaa taagaagcct
     2821 gtcacccctg aaccgttcgg ggaggattca gtgaatgatc ttccaccctt tgacccgaag
     2881 ctctctctgt cgaactacaa atttccaacc ctggcgctgc ttcgtgatta ccggagcccg
     2941 ggagccacca ccaatgacga ggtgaatgcc aacaaggaga taatcctcaa aacactcgct
     3001 gaccacaaga tcaccatcag gcagatatcg gcaacagtgg ggcctaccgt gacactgtac
     3061 gaggtggttc ccgacagggg tgtcaagata caccgcatca agtcgcttga caatgacata
     3121 gccctgagtc tctctgctct gggaatcagg atcattgccc ctatccccgg acgcggcacc
     3181 gtgggcatag aagtacccaa caagaaacct gaaacggtat cgatgaaagg gctgattgca
     3241 tcgcgtgaat tcattgacac taagttcgaa ctgccgctgg ccatgggacg aactatcaca
     3301 aatgacccct atatcattga tctcacgaag atgcctcacc tcctcgtggc aggcgccaca
     3361 ggacagggca aatcagtggg actgaatgca atcatcacct cgctgctcta caagaagcac
     3421 ccggctgaac tgaaactggt gctcatcgac cccaaacggg tcgaactccc gctctacgcg
     3481 aagatcgaac ggcacttcct tgcaaagctt cccgatgagg aagatgccat tgtcactgac
     3541 accaggaagg tgatcaatac tctcaattca ctttgcatac tgatggatca ccgcctggag
     3601 ctgctgaaac tgtcacagac aaggaatata aaggaataca atgacaagtt cattgaacgc
     3661 cggctcaatc ctgaaaaagg acatgacttc
//
LOCUS       DAOPBK010000214         3613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_20796_length_3613_cov_4.577853, whole genome shotgun sequence.
ACCESSION   DAOPBK010000214 DAOPBK010000000
VERSION     DAOPBK010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20796_length_3613_cov_4.577853"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..411)
                     /locus_tag="PLA17_10215"
     CDS             complement(22..411)
                     /locus_tag="PLA17_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85827.1"
                     /translation="MKRVIMFGLFLLTTAGLFSQTFRSRSATIRSDGFEIDRTVRSIV
                     LTDKRITITNYLSSNTKPLVLNVYHTEDKEDRFDGVCRYYYCTATNDEKLSRYHKILV
                     IKKPYSITLELYLADDNKYVHDLEING"
     gene            complement(828..2936)
                     /locus_tag="PLA17_10220"
     CDS             complement(828..2936)
                     /locus_tag="PLA17_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPK85828.1"
                     /translation="MMKTNRHLLSVFALLLAMGIVISCTNGAKKKVQPVLGTRSVQII
                     EDKGLQFKDLNKNGELDKYEDWRLSDDERIADLVSQMTLEEKVGLMFHPNIAVPADGI
                     VKYDFTEEEKAAIATAANEQYAGPIGPGGQAPAGGGMAMGQMRRTATAKSYIEEKNFR
                     NILNNGVAPIREFAAWSDKMQEIAEATRLGIPIMFSTDPRHGAVLGAHVSGKQYLSQW
                     PGKEGQVGITASRDTAVVRKFGEVVAEEYRAIGLHMILGPQIDVMTEPRWGRNMGCFS
                     EDANLTSEMLAAFMDGAQGKEAGPGKILVHLKHWPGSGPHKDGTGQWLVYPGDNFDYH
                     LIPWKTGIAKGALAAMGYYSGTCYDSLNVNYSHYMSTEVLYNQLGFKGAICTDWGVVG
                     RGPLKPGLQGNTTLKDNMEMIINAGVDQMGSETNNNLVIELVKEGRVTEERINQAASR
                     ILQWHFKLGLFENPYVDPEAAVNIVRSDKNQELGYLAQLESIVLLTNDGVLPAKENIK
                     IFVEGIDRELAAEYASVVDDPNQADLILVRTSTQAERGFGGGPASQAGNTGNRPGTTV
                     GQTGNRAAAGQQRNAQQMTANMNPFAAREVDITFPSVKWDAIKALAGTGKPVVVAFNP
                     SGTGCVLPPDLKQVVKGTIMTFDVLDNALLDVVFGKFNPIGKLPFEIPLSMDDVRNQL
                     EDVPFDAPNKAFEFGYGLSY"
BASE COUNT          962 a          883 c          724 g         1044 t
ORIGIN      
        1 gccggatggc agaattatct tttatccgtt tatttccaga tcatggacat acttgttgtc
       61 atctgccaga tacagctcaa gggtgattga atagggtttc ttgatcacca gtatcttgtg
      121 gtagcgacta agcttttcat cgttcgtcgc ggtacaatag tagtatctgc acactccgtc
      181 gaagcggtct tccttatctt cagtatggta cacattcaat accagcggtt tcgtgttgct
      241 gctcaggtaa ttggtgatag tgatgcgctt atcggtgagc actattgacc tgacagtacg
      301 gtctatctca aaaccatcgc ttctgattgt ggcagacctg ctcctgaatg tctgtgaaaa
      361 caaacctgca gtagtcagta aaaacaaacc aaacatgata acccttttca tgccagaaga
      421 tttaaatcca tattttgact aacgccaggt tatacgtgtg aatcaggatt ttgttctgct
      481 ttttgctgac aaatgagatt tgccggcact aaaattataa ttcctgacgc cacgctcttc
      541 ttagtagcat gcacggatag gtcaccctaa agatgtcaat acgctggctg atacatggat
      601 gagccgcctc ttgcaaggta tattcaataa gggcacacta aaaaacacaa gaggccggtt
      661 ttgacctctt gtgtctgtaa agtgagagtg tgattgccct gctacgccat ggcttcgcag
      721 ggcgaagcac cccggagagg atgaaagttg cggctgatta atacactcct caggcagatt
      781 caggaatgaa tcaaatctga ccggtcacat aatacattta tcagctttca ataagataat
      841 ccgtaaccaa actcaaaagc cttgttcgga gcatcaaaag gcacatcctc caattgattc
      901 cttacatcat ccattgacaa tggaatctca aacggaagtt ttcctattgg attgaatttg
      961 ccgaaaacaa catcaagcag tgcattgtca agaacgtcaa aggtcataat agttcctttt
     1021 acaacctgtt ttaagtcagg tggcaatacg caacctgtac cggatggatt gaaagcaaca
     1081 acaaccggtt tgccggttcc ggcaagtgcc ttgattgcat cccatttaac agatgggaac
     1141 gtaatgtcaa cttccctggc agcgaaagga ttcatatttg ctgtcatttg ctgagcattt
     1201 ctctgctgac ctgctgcagc cctgtttcca gtctgtccga ctgttgtacc tggtctgttg
     1261 ccagtgttgc ctgcctgaga tgcaggacct ccaccgaaac ctctctctgc ctgtgttgat
     1321 gttctgacaa gaataagatc tgcctggttt ggatcatcaa caacgctagc atattctgct
     1381 gccagttccc tgtcaattcc ttctacaaaa atctttatgt tttcctttgc aggaagtaca
     1441 ccatcatttg taagcagaac tattgattca agctgagcca gataccccaa ttcctggttt
     1501 ttatcactcc tgactatatt aacggctgcc tcaggatcaa cgtaaggatt ttcaaagagt
     1561 cccagtttaa agtgccattg aagtatcctt gatgctgcct gatttatcct ctcttctgtt
     1621 actcttccct ccttcacaag ctcgataacc aggttgttat ttgtttcact tcccatctgg
     1681 tcaacaccgg cattgattat catttccatg ttatctttca gcgtcgtatt cccctgtaaa
     1741 cctggcttga gcgggccgcg gcccaccaca ccccagtcag tgcagatagc tcctttgaaa
     1801 cccaactgat tgtataaaac ttcagtcgac atgtagtggg aatagttcac attcagtgaa
     1861 tcataacagg tgccgctgta ataacccatt gcagcaaggg ctccttttgc tatacctgtt
     1921 ttccagggga tgagatgata atcgaaatta tcacctggat agaccagcca ttgtccggta
     1981 ccatctttat gcggacctga tccgggccag tgtttcaaat gaaccagtat cttgccaggc
     2041 cctgcctcct taccctgggc accatccatg aaggcagcaa gcatttccga agtaaggttg
     2101 gcatcttctg aaaaacagcc catattgcga ccccatcttg gttctgtcat aacatctatc
     2161 tgtgggccca ggatcatatg caaccctatt gcacgatact cttctgccac aacctcgcca
     2221 aatttcctga caacagccgt atcacgggat gcagtaatgc caacctggcc ttccttgccc
     2281 ggccattgtg agaggtattg ttttccgctt acatgagcgc ctaaaacagc cccgtgcctc
     2341 ggatcggttg aaaacattat tggtatacca agccttgtag cctccgcaat ctcctgcatc
     2401 ttgtcagacc aggcagcgaa ttcacggata ggagcaacgc cattattgag gatattacgg
     2461 aaattctttt cttctatata tgatttagcg gttgctgttc ttctcatctg gcccatagcc
     2521 atgccaccac ccgccggtgc ctgtccgccc ggaccaattg gacctgcata ttgttcattg
     2581 gcagcagtgg caatagcagc tttctcctcc tctgtgaaat cgtattttac aattccgtca
     2641 gcaggaacgg caatattggg atggaacatc agaccgactt tctcttcaag tgtcatctga
     2701 ctaacgagat ctgcgattct ttcatcatct gatagtcgcc agtcctcata tttgtcaagc
     2761 tctccatttt tgttcagatc cttgaactgc agacctttgt cctcaataat ctgcacagat
     2821 cttgtcccaa gtacaggttg cactttcttt tttgcaccat tagtgcatga aataacaatt
     2881 cccattgcca gaagcaatgc aaaaactgat aacaggtgtc ggtttgtttt catcataata
     2941 taattctgtt taatcttatt tggctccggc catgataggt tgaaacaagc tcatcaggat
     3001 atacagttct tccatccggg gattcataag aactattcct gccagccttt tgcaatttac
     3061 attatatctc attcaaattc aaatcaatta ccatcaataa aatgaataat cagaatgact
     3121 tttcgcaagg gatgatattg ttttaaatca ttctctggtt gcaacccgga caactaattt
     3181 aattttccgg gtttaatagt tggaactccg ataaaaagcc agacggaata aaaagtcgcc
     3241 attatacgga ataaaatgcc gccaaaacac ggaatccata aacagattga agaagcagca
     3301 accctgagcc attgaaaatc agtttttttc agtctctcat ctggcaggaa tttcagaagg
     3361 ggccactaaa aaaacacaat aggccggtta ttgcctcctg gccaaaagct tcctgagtga
     3421 tcaggataac tgtcaaaaaa aagaggctgc cccattttat tataagacag cctcctcttt
     3481 caggtgtgag agtgagtctt gaggaaaaag agtgacagtg ctcacgaatg ctctcgcact
     3541 cgctctcact tcttttctgt acccggagcg ggacttgaac ccgcacaggc attactgccc
     3601 actggatttt aag
//
LOCUS       DAOPBK010000215         3607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_20839_length_3607_cov_4.455236, whole genome shotgun sequence.
ACCESSION   DAOPBK010000215 DAOPBK010000000
VERSION     DAOPBK010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20839_length_3607_cov_4.455236"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1237)
                     /gene="nagA"
                     /locus_tag="PLA17_10225"
     CDS             complement(125..1237)
                     /gene="nagA"
                     /locus_tag="PLA17_10225"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPK85829.1"
                     /translation="MYTLFYNAHFFTGIGLVEGKELLIRDDRIEGFADPGSVSADARR
                     IDCGGNLVVSGLIDLQIAGGGGYLFSSHPTPGALDTIIRSIVSTGTTGFLLALPTNSR
                     EVYTAAFRTVSGCAHPAVMGLHMEGPFISNARRGAHVPELIMMPTPENVSSLLDEAGS
                     TIRMMTVAPEICTPEVIALLRERGITVAAGHSNATFREAMAGFEMGIETTTHLFNAMS
                     PLHHRDPGLPGAAFLSGTACASIIADGIHVDYATVNIARKIMRERLFLVSDAVEENDR
                     GAYRHVKRSDRYTLPDGTLSGAKLTMLDAVRNSIDRAGIEKYEAFRMATLYPARLIRA
                     SDRGMIEPGARADLVIIDNELNLKGVCLNGITEFKE"
     gene            complement(1270..2358)
                     /locus_tag="PLA17_10230"
     CDS             complement(1270..2358)
                     /locus_tag="PLA17_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HPK85830.1"
                     /translation="MTFIYLSIWISALIGVILIAWIRSFDIYEKEAFIAMLWAFIAGG
                     ITSVMIALGAYEFLRAFGLNDEVISNALGSLLVIGPVEEFAKLLGLVVVYSLIRKQFN
                     ELTDGIIYMSCVALGFSIIENYFYANAGENSQYLLVYRAFISTPAHISFSVIIGYAWY
                     RYKKENKPFSTVIVALLIASLLHGIFDALAFTPGYNLLLLLYLWFIIMQSLRLVQYTN
                     VISPFRPRFEALLETPSGETAHGVECPNCGSSAPKELFINSYFTSCRCDSCGNHIASR
                     NDIRRIFRIFAPEYKRLLRKLVPVRFSDGRIVMSVYGSAFFNSSGNAGFFRVSDVARK
                     LQAINDDLLDRFRKRSFISANLLKQFFE"
     gene            complement(2479..>3607)
                     /locus_tag="PLA17_10235"
     CDS             complement(2479..>3607)
                     /locus_tag="PLA17_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPK85831.1"
                     /translation="WPDSAMFTTDEIIHNLRYLKQASSFRTRYDYDNLLYIVAGEIVA
                     RVSGQSWEEFTESRIMKPLGMNESAASFRRIKDLSNIIDAHVTVDGRLKVVPKQEAKR
                     HNSVGGIYSNISDMSKWVILQLNRGRHGSSAGEKIFSEEIHREMWSPQTVKRVGTTGP
                     YRSNFAFYGLGWNLTDINGYLQVYHTGSHAGIETRVTMLPELNLGIIVFTNQQVPEAH
                     QAITNSIIDGYLGISGYDHVSRLREKVEREQAMAREITDEVWATVERRRMESSQEIEA
                     ESFAGTYSDAWFGDIIISAENGQLRFRAEKSPRMRGGMLHYTANTFIVKWDDRSMDAD
                     AFAVFTLDREGKPVSFTMEAISPLTDFSFDFQDLYLVRSEK"
BASE COUNT          882 a          999 c          805 g          921 t
ORIGIN      
        1 cacctcgtct ccctggcggt tttccagcgg taatacctcc cttgccctga tgttgccctc
       61 acggtcaaac agtgcaaggg caagcttcgt tcctccaaga tctattccca gcaatgccat
      121 tttatcactc tttaaattca gttattccgt ttaaacaaac tcccttgaga ttcaactcat
      181 tatcaataat aaccaggtca gccctggctc ccggctcaat cattcccctg tcactggccc
      241 tgatcagccg tgcagggtag agcgtagcca tcctgaaggc ttcatacttt tctattcctg
      301 ccctgtcaat gctgttcctt actgcatcaa gcatcgtcag cttcgctccc gaaagagtgc
      361 cgtcaggcag tgtgtaacgg tcagatcttt tcacatgcct gtatgccccg cggtcattct
      421 cctctactgc atcggatacg aggaacagcc tctcccgcat tatctttctg gcaatgttta
      481 ccgtggcata gtcaacatga attccgtccg cgatgatgct tgcacatgct gttccggaga
      541 ggaatgccgc tccgggcaga cccggatcac ggtgatgcag aggcgacatc gcattgaaca
      601 gatgcgtcgt ggtttcaatt cccatctcaa agcctgccat tgcctccctg aatgtggcgt
      661 tgctgtgtcc ggcggcaacg gtaatacctc tctccctgag aagagcaatt acctccgggg
      721 tgcatatctc aggcgccaca gtcatcatcc ggatggtgct gcccgcttcg tcaagaagtg
      781 agcttacatt ttccggagtg ggcatcatta tcagctcagg aacgtgcgcc ccgcgccgtg
      841 cattgctgat gaacggcccc tccatatgca ggcccatcac cgccggatga gcacagcccg
      901 agactgtccg gaaggcagcg gtgtatacct ccctgctgtt ggtgggcagc gcgagaagga
      961 agccggttgt gccggtagag actattgatc tgattatcgt atcaagcgcc ccgggagtag
     1021 ggtgcgatga gaaaaggtaa ccccctcctc ccgctatctg aaggtcaatc agtcctgaaa
     1081 caaccaggtt gccaccacaa tcaatacggc gggcatctgc cgacactgaa cccggatcgg
     1141 caaaaccttc gatccggtca tccctgatga gcagttcttt cccctcaacc aaccctatgc
     1201 cggtgaaaaa gtgtgcgttg taaaaaagag tgtacatctt gaatctccga tatctcaatt
     1261 actcattttt cattcaaaaa actgcttcag caggttggct gatatgaatg accgctttct
     1321 gaacctgtcc agcaggtcgt cattgatggc ctgcaacttt cgggcaacat cacttaccct
     1381 gaaaaacccc gcatttccgc tgctgttaaa gaaggcagag ccataaacac tcattactat
     1441 ccggccgtca ctgaacctga ccggcacaag cttccttaaa agccttttgt actcgggagc
     1501 aaagatcctg aaaatccttc ttatatcatt gcgtgatgct atgtggttac cacagctgtc
     1561 gcaccggcag gaggtaaagt aactgtttat gaagagctcc ttgggcgccg atgaaccgca
     1621 gttcggacac tcaacaccgt gagctgtctc acctgaggga gtctccagca atgcttcgaa
     1681 acgaggcctg aagggcgaga tgacatttgt gtactgaacc agcctgagcg attgcatgat
     1741 tatgaaccag agatataaaa gcaacaggag gttataaccc ggagtgaatg caagagcgtc
     1801 aaagattccg tgaaggagac tggcaatcag cagtgcaact atcactgtac tgaacggctt
     1861 gttctccttc ttgtacctgt accatgcgta tccgatgatg acactgaatg agatatgtgc
     1921 cggagtgctt atgaaagccc tgtagacaag cagatactga ctgttttcac cagcattggc
     1981 atagaagtag ttctcaatga tggaaaaccc cagtgccacg catgacatgt atatgatccc
     2041 gtcagtcagc tcattgaact gcttcctgat cagtgaataa acaaccacca gaccgagaag
     2101 ctttgcaaac tcctcaaccg gacctatcac caggagcgaa ccaagggcgt tgcttatgac
     2161 ttcatcatta agtccaaaag ccctgagaaa ttcataggct ccgagggcaa tcatgaccga
     2221 ggtgatgccg ccggcaataa aagcccacag catggcaatg aaagcctctt tttcatagat
     2281 atcgaaactg cggatccatg caataaggat aactcctatc agtgcggaga tccagatact
     2341 caaataaata aaggtcatca gttcggtttt ctgatttgat tgcaaaaata acgtttatgt
     2401 accgcttgca taagaaccct ggttactttt aactttccac ttgtcacttt caacttttaa
     2461 cttcccactt ttaacttgtc acttttcact tctgacaagg taaagatcct gaaagtcaaa
     2521 gctgaaatcg gtgagagggg agatggcttc catggtgaat gagaccggct ttccttccct
     2581 gtcaagggtg aatactgcaa aggcatcggc atccatgctc cggtcatccc acttcacgat
     2641 aaaagtgttg gctgtatagt gcagcatccc gcccctcatc ctgggtgact tttcagccct
     2701 gaacctcagc tgcccattct ctgcactgat aataatatca ccgaaccagg catcactgta
     2761 ggtccctgca aatgactcag cctcgatctc ctgagacgac tccatcctcc ttctctctac
     2821 agttgcccag acctcatcgg taatctccct tgccatggcc tgttcacgtt ccaccttctc
     2881 cctgagtctc gaaacatgat catagccact tatcccgaga tagccgtcaa ttattgaatt
     2941 ggtaattgcc tgatgtgcct ccggcacctg ctggttggta aagacaataa tccccaggtt
     3001 gagttcggga agcatagtta cccttgtctc aatgccggca tgacttcccg tatgatagac
     3061 ctgcaggtat ccgtttatgt cggtgaggtt ccatcccaga ccatagaatg cgaagttgct
     3121 cctgtatggc cccgttgtgc cgactctctt cacggtctga ggactccaca tctccctgtg
     3181 aatctcctcc gaaaaaatct tctctccggc actgctgccg tgcctccccc tgttgagctg
     3241 cagaatgacc cacttactca tgtcactgat attactgtag atgcctccca ccgagttgtg
     3301 cctcttggct tcctgtttgg gaaccacctt cagcctgccg tcaaccgtca catgggcatc
     3361 aataatattg gaaaggtctt ttatccgcct gaaggaagca gcagattcgt tcatcccgag
     3421 aggtttcatg atacgtgact cggtaaactc ttcccagctc tgtcccgaga ccctcgccac
     3481 aatctcgccg gccacgatat agagcaggtt atcatagtca tatctggtcc ggaaagatga
     3541 tgcctgtttc aggtacctga gattatgtat tatctcatct gtggtgaaca ttgccgaatc
     3601 aggccat
//
LOCUS       DAOPBK010000216         3599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_20890_length_3599_cov_5.087190, whole genome shotgun sequence.
ACCESSION   DAOPBK010000216 DAOPBK010000000
VERSION     DAOPBK010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20890_length_3599_cov_5.087190"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1088)
                     /locus_tag="PLA17_10240"
     CDS             complement(186..1088)
                     /locus_tag="PLA17_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85832.1"
                     /translation="MRHFLMVAVAGCAVILLTASCNNTDKEIKTPPPVFSTYIPESPD
                     DTTHVREIYYGLMTPVEVCNIFERLGIHFNDTIILPPGNRDLYMSSYKAAMNLGVYGV
                     DMGYVRLFGVNRQTMSYFNILKTLSGRLNIPDSFISDAIKSLDPEMSHHDSVTRLMND
                     VYSKIDYHLRQEGNEGTLGLMLMGGWIEAMYLATQLAYDPANPDPQVVGKIAEQKYSL
                     ISLLSFMKNYYNDPMVVFYTKKLKYLNRWFDKFEIYYQPGDITVDTLRQVITTSGTEM
                     TVTVDALNEIRDYVAKLRAEITAV"
     gene            complement(1342..3303)
                     /gene="dnaG"
                     /locus_tag="PLA17_10245"
     CDS             complement(1342..3303)
                     /gene="dnaG"
                     /locus_tag="PLA17_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPK85833.1"
                     /translation="MIDHSTIERIFDAAQITEVVSEFVSLKRRGVNLLGLCPFHNEKT
                     PSFTVSPAKGIFKCFGCGKGGNAVNFIMEHEHLSYPEALRWLAKKYHIEIEEREVTPE
                     ELAKQNERESILVVTQYAARQFTENLFHGHEGMAVGLSYFRERGFADAVLRKFEVGYC
                     NERRDDFTKKALAAGYKSEFLVSSGLTIDKEGYMFDRFAGRIMFPIHSLSGQVLGFGG
                     RIIKTDPKAAKYVNTPETEVYHKSRILYGIFQARQAIIKEDRCYLVEGYTDVLSMHES
                     GVENVVASSGTALTQEQIRLIKRFTPNITMLYDGDSAGIKASLRGTDMVLEEGMNVRI
                     VVLPDGEDPDSYSKKVSDEEYRRYLKDNETDFIRFKTRMLLDEAQSDPVMRASLVREV
                     VRSIAVIPDQITRTVYVKECSTQLDMPEEVVLDEVMKLRRQESFRNRNAWPGQEASGS
                     SMSDLTVTTAPSQRQKKKEETSYYSEREVIRMLIRYGSEVLTRHTDRETGTETITTVA
                     DYIISEINEDGLTFEDPLFAEMYDEITFSVSQGLIPGDRNFVHHANPAISAVTVDMLT
                     EKDTLSQIWRKHGAYVETEDMKIREIVPDSVLKFKGDKIKMMQKEIRKAMAEATGDDD
                     RISELQQQYKAMTLLQKKISAGLGGRLII"
     gene            3337..>3599
                     /locus_tag="PLA17_10250"
     CDS             3337..>3599
                     /locus_tag="PLA17_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK85834.1"
                     /translation="MNTGRKPLMVRGARQVGKTYSIEKFARENYSYLLKVDLEQDRNI
                     HSLFESLRPEQIMNELQYSMPVGRLDYAYMYPLNLSEFLTAAG"
BASE COUNT          786 a          920 c          964 g          929 t
ORIGIN      
        1 aacgatgcct gatgtgtagg cggagatgta tttactgaat cccttgtcaa tgatggtgat
       61 ggcaggagag acagctccgg agccgcgctc tttctttccc tgcccgcatg agatcaaaat
      121 gacggcaata acagccgtga tgaggagaaa acgacggttc atagtatatt ttgtttcagg
      181 tttattcaga cagcagtgat ctcagccctg agcttcgcca cataatcacg tatctcattc
      241 agtgcatcca cggttacggt catctccgtg ccgctggtgg tgatgacctg ccgaagggtg
      301 tcaaccgtga tatctccggg ctgataataa atctcgaatt tatcaaacca gcgattgagg
      361 tacttcagtt tcttggtgta aaaaacgacc atcgggtcat tgtagtagtt cttcatgaaa
      421 ctcagcaggg agatcagcga gtacttctgt tcggcaatct tgcccaccac ctgcggatca
      481 ggatttgcgg gatcataagc cagctgtgtg gcaaggtaca tcgcctctat ccatcctccc
      541 atgagcatca gtccgagggt cccctcgttg ccttcctggc ggaggtggta atcaatcttg
      601 ctgtaaacat cattcatcaa tcgtgtcact gagtcatgat ggctcatctc cggatcaagg
      661 ctttttattg catcggagat gaaggagtca ggtatattga ggcggccgct gagtgtctta
      721 agtatattga aatagctcat tgtctgcctg ttgacgccaa agagcctgac atagcccatg
      781 tcaaccccgt atacaccgag gttcattgcc gccttgtaac tgctcatata tagatcgcgg
      841 ttccccggcg ggaggatgat tgtgtcattg aagtggattc cgagtctctc gaagatgttg
      901 cacacctcca cgggtgtcat gagaccgtaa tagatttctc tcacgtgtgt ggtatcatcc
      961 ggagattcgg ggatgtatgt tgaaaagacc ggcggcggcg ttttaatttc tttgtcagta
     1021 ttgttgcatg atgcggtgag cagaatcact gcgcatccgg caacggccac catcaggaaa
     1081 tgtctcattg tatgcagaaa tttgccacaa gttagcaaaa aagtgctcaa tatggttata
     1141 taatatttta ttgcctgcgg cgaaaagcgg gggattgatc tcaggtgtta ctgtgttcta
     1201 agctccggca gtgagaagtg agggtaatcg gtgccggtgc agttcttaat agtccctgga
     1261 gaaaagtggt ggattgcctc tgccgttacg attctcaata gtctgtggcg aaaaacggag
     1321 gattggcggc agcaggcgtg atcagataat caatcttccc cccaaacccg ctgatatctt
     1381 cttttgcagc aatgtcatcg ccttgtactg ctgctgcagc tcggagatcc tgtcatcatc
     1441 gccggtggcc tctgccatcg ctttgcgtat ctccttctgc atcatcttga tcttgtctcc
     1501 cttgaacttg agaaccgaat cgggaacgat ctccctgatc ttcatatcct ctgtctcgac
     1561 atacgcgcca tgttttcgcc agatctggct gagggtgtcc ttttccgtca gcatgtcaac
     1621 ggtgacggcg ctgatggcgg gattggcatg gtgcacgaag ttcctgtcgc ccgggataag
     1681 gccctgactt accgagaagg tgatctcgtc atacatctcc gcgaagagcg gatcttcaaa
     1741 cgtcagtccg tcttcattta tctcagatat tatataatca gccacggtgg tgatggtttc
     1801 agtgcctgtc tccctgtcgg tgtggcgggt gagtacctcc gatccgtagc ggatgagcat
     1861 ccggatgacc tccctctcgg aataatatga tgtctcttcc tttttcttct gacgctgcga
     1921 aggggcggtg gtcacggtca ggtcagacat gctgctgccg gaggcctcct gtcccggcca
     1981 ggcgttgcgg ttgcggaagc tctcctgcct gcgcagcttc atcacctcgt caagcaccac
     2041 ctcctcgggc atgtcgagct gcgtgctgca ctcctttaca taaacggtgc gggtgatctg
     2101 gtcagggatg accgcgatgg agcggaccac ctcgcgcacc aggctggcac gcatgacagg
     2161 atcactctgt gcctcatcaa ggagcatcct tgttttaaac cgtataaagt cggtctcgtt
     2221 atccttcagg tacctcctgt actcttcgtc cgagaccttt ttcgagtagg agtcagggtc
     2281 ttcgccgtca ggcagaacta ctatccgcac gttcatcccc tcctccagca ccatgtcagt
     2341 gcctctgagt gaggctttta tgccggcaga gtcgccatcg tagagcatgg tgatgttggg
     2401 ggtgaagcgc ttgatgagac gtatctgctc ctgcgtcagg gcggtgccgg agcttgccac
     2461 cacgttctcc acgcccgact catgcatcga gagcacgtca gtgtaaccct ccaccaggta
     2521 gcagcggtcc tctttgatga ttgcctgccg cgcctggaaa atgccgtaga ggatgcggct
     2581 cttgtggtaa acctcggtct cgggggtgtt cacatatttg gctgccttcg ggtcggtctt
     2641 gatgatacgt ccgccaaaac ccagcacctg ccctgagagc gagtgtatcg ggaacattat
     2701 gcgtcccgcg aagcggtcga acatgtaccc ttccttatct attgtgaggc cgctggagac
     2761 caggaattca ctcttgtagc ctgcggcgag ggcttttttg gtgaagtcgt cgcgccgctc
     2821 attgcaatag cccacctcga acttgcggag gacggcatcg gcgaagcctc tctcgcggaa
     2881 ataggaaaga cctacagcca taccctcgtg gccgtggaag aggttctcag tgaactgccg
     2941 ggcggcgtac tgcgtcacaa cgagaatgct ctcgcgctca ttctgtttgg ccagctcctc
     3001 gggggtgacc tcacgctcct ctatctctat atggtacttt ttggccagcc agcggagcgc
     3061 ctcagggtac gagaggtgct catgctccat gatgaagttg acggcattcc cccccttgcc
     3121 gcagccgaaa cacttgaata tccccttggc aggggaaacg gtgaaggagg gggttttctc
     3181 attgtggaac ggacacagtc ccaacaggtt gacccctctt cgcttcagcg aaacgaactc
     3241 ggaaacgacc tcggttatct gcgccgcgtc aaagatccgt tctatggtgg agtgatcaat
     3301 catgggagga taaatgtact aaaaaaggtg cttgtaatga acaccgggag aaaaccatta
     3361 atggtaaggg gagcgcgcca ggttggtaaa acatacagca tagagaagtt tgccagagag
     3421 aattatagtt atctgcttaa ggttgacctg gagcaggaca gaaacatcca ttcactcttt
     3481 gagagcctcc ggcctgaaca gataatgaat gagttacagt attcaatgcc tgtcgggagg
     3541 cttgattatg cctatatgta cccgttgaat ttatctgaat ttctgactgc ggcagggga
//
LOCUS       DAOPBK010000217         3568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_21111_length_3568_cov_5.792485, whole genome shotgun sequence.
ACCESSION   DAOPBK010000217 DAOPBK010000000
VERSION     DAOPBK010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21111_length_3568_cov_5.792485"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..2509)
                     /locus_tag="PLA17_10255"
     CDS             complement(221..2509)
                     /locus_tag="PLA17_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK85835.1"
                     /translation="MKKLIFIMLLLAVCSLINGQRLRIEGFVLDAQTGIPLPDAVIKV
                     DEGRYLTDIRGRFSFYHPAGSLTLHIKYGGYEEMKSDLTVRNDTTLMLYYDKLVSIEE
                     IRVTGSRKPGFKRDSNIEITGFAPAEVSFLPVIGSGEDLLKKIQYLPGVQSGGEGSAG
                     LIVRGGQYDQNLFNLNGFPVYQPFHFSGLISAIDPFMVSEVEVMKGGFPARYGGKLSS
                     VVNFNTDRSVSDTVLTSVSAGLLVSGAGVAFSPDSVTTVAVAGRIGTTKFLNKTLNRS
                     FPDFPFFDFHDLNLYASRDIGNDNDLCLTLYFNRDKLEKYTSYTEFDDDVETLYENRV
                     ISGWKDMLAGISWTNKPDANKSITTRLFFQDFLSESGEEIHLTRYEEETQTEDAINTT
                     SSRIKELGFNSTVAISNKVHNLEAGIFTNLRSVKPVIGTFLYTNGELVNHPAGENASL
                     GGISQLEAGAYIEDRITFNDRFTLRPGIRLTLLSDLKSAYFLPEPRLLASFKVREDLT
                     LMGAYTLSSQSIHTVSSSNVMAVNDVWIPSYGNIKPSRTQQVEVAAFFNPGTFFRGEV
                     NLFLKSSRDVYMYREGASFVLYPRWEDNIIPATGLGYGAEFLLQAELHKTYALLTYTL
                     SKTTRQSPEINNGERFDHKYDRPHDFRLSLGYKINHKLSISGSFVMQSGTVFSFYDRI
                     IQGDLPFLERINNIRFPLYHRLDIGLERKTSARWGRKTFRVDIYNAYSKMNPWYLTLD
                     NGELQQVTLFPIIPSVSYKIEF"
     gene            complement(2520..>3568)
                     /locus_tag="PLA17_10260"
     CDS             complement(2520..>3568)
                     /locus_tag="PLA17_10260"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HPK85836.1"
                     /translation="TALRVIGLIIIIGGICSCSRDMTFSLPETEPLLAVQSIIGPDKD
                     SEVRVAMARGIQHPHIQMDIECKVDMFEDGVFYKRLILDTPVIASGTMTNKGYLVYPA
                     ESSLALTEGREYSVEVNYPGFETVTARDIKPNAVKINSISWRFFTGEMPEWYYNTPQK
                     RYQNTFDDNWQTNRDTALIEYVINFDDPPEIGDFYQIGINYLVPDNYSIPRDTIHYRI
                     QYAIVSDPDPPYVIFNYRKNKSQASGTGNTKNGNSMIIYQTTWELLWKDDEFDGSRHS
                     ITIIATRPDEGAKCIISLYSLSENYYRYMIDRWRYCKLLHDPFSEPIRFHSNTSNGCG
                     IFAMYSVDKDTLQF"
BASE COUNT          959 a          781 c          808 g         1020 t
ORIGIN      
        1 tatctcggtc tggaccaata aatgattgaa cagctaacag aggttctgtt tcaggtaagc
       61 tgaaggtcat atcatcggag catgaggaga ggataaacag aatcaccagg caggatgaat
      121 aaatggtata tggtgtggtg aatggttgtt tcatcatatt atagtttcga ttttaaaggg
      181 tatgatggag cccgcatgac tgaaaacttc ttctgtttgc ttaaaactct attttgtaac
      241 tcacagatgg tataatgggg aacagagtca cctgttgcag ttcaccatta tctagtgtca
      301 gataccatgg attcattttc gaataggcat tgtaaatgtc caccctgaac gttttccttc
      361 cccacctggc ggaagttttt cgttcaagcc cgatatcaag cctgtgatat aacgggaacc
      421 tgatattgtt tatcctttcc aggaatggca gatcaccctg tataatcctg tcgtaaaacg
      481 agaatacagt cccgctctgc atgacgaaac tgccactgat actaagttta tggttgatct
      541 tgtagccaag ggagagcctg aaatcgtgag gcctgtcata cttgtgatca aatctctcac
      601 cattatttat ctcaggcgat tgccgtgttg tcttagaaag ggtgtaagta aggagtgcat
      661 aggttttatg cagttcagcc tgaaggagga actcggcacc gtaaccaaga ccggttgccg
      721 ggatgatatt atcttcccat ctaggataga gtacgaagct tgcaccttcc ctgtacatgt
      781 aaacatccct gctgctttta aggaacagat tgacctcacc gcggaagaat gtcccgggat
      841 tgaaaaaagc agctacctca acctgctgtg tcctggatgg ctttatattg ccataggagg
      901 gaatccacac atcgtttact gccattacat ttgaagaact gacagtatgg atcgactgtg
      961 aggaaagggt ataggcaccc atcaaagtca gatcctctct gactttaaag gaagccagca
     1021 gcctgggctc tggtaaaaag taagcagatt taaggtctga taaaagggta agccttattc
     1081 ccggccggag tgtgaacctg tcattgaatg tgattcggtc ttcaatataa gctcctgctt
     1141 cgagctggga gattcctccg aggctggcat tttcacctgc cgggtggttt acaagttccc
     1201 cgtttgtata gagaaatgtg ccaataacag gcttcacaga gcgtagattg gtaaagatgc
     1261 ctgcctcaag attatggact ttatttgaaa tggccacggt gctgttaaaa ccaagctctt
     1321 tgatcctgct gctggttgta tttattgcat cttcagtctg agtttcttct tcatagcggg
     1381 tcagatggat ctcttcaccg gactctgaca ggaaatcctg aaagaacaga cgagtggtaa
     1441 tgcttttgtt cgcatcgggt ttattggtcc atgaaatccc tgcaagcata tctttccaac
     1501 ctgagatcac tctgttctcg tacagagttt caacatcatc gtcgaattct gtgtagctcg
     1561 tgtacttctc aagcttgtca cggttgaaat agagggtaag gcataagtca ttatcatttc
     1621 cgatatcgcg tgatgcatac aggttcagat catgaaaatc aaaaaacggg aaatcaggga
     1681 aactgcggtt aagtgttttg ttcagaaatt tggttgtgcc gatcctgccg gccactgcaa
     1741 ccgttgtaac ggagtcgggt gaaaaagcaa ctccggcgcc cgataccagc agcccggctg
     1801 aaacggaagt aaggactgta tcagagacac ttctgtcggt attgaaatta accactgatg
     1861 acagttttcc cccgtacctt gcagggaatc ctcccttcat cacttccacc tctgagacca
     1921 tgaaaggatc aatagccgag atcagtcccg aaaagtgaaa tggctgatag accgggaatc
     1981 cgttaagatt aaaaaggttc tggtcatatt ggcctcccct tacaataagc ccggccgacc
     2041 cttctcctcc tgattggacc ccgggcaggt actggatttt tttcaagagg tcctcaccgc
     2101 tgccgatcac gggcaggaag ctcacctcgg caggggcaaa gccggtgatc tcaatattgg
     2161 agtctcgttt aaaaccaggc ttacgggatc cggttactct gatctcttcg attgagacca
     2221 gtttgtcata gtatagcatc agggtagtat catttctaac ggtcaaatcc gatttcatct
     2281 cttcataccc gccgtactta atatgcagtg taagtgagcc ggcaggatga taaaatgaaa
     2341 acctgcccct gatatctgta aggtacctgc cttcatcgac tttaatcact gcatcgggaa
     2401 ggggaattcc agtttgggca tcaaggacga atccctcgat ccgaaggcgt tgcccgttga
     2461 ttaatgagca tacagccaac agaagcatta tgaatataag ttttttcatt cctcaacgat
     2521 taaaattgca gggtatcttt atcaaccgag tacatggcaa aaataccgca tccattactg
     2581 gtattgctgt gaaatctgat tggctcagag aatggatcat gtaataactt acaatacctc
     2641 cacctgtcaa tcatatacct gtagtaattc tcactcaggg agtataggga aataatgcat
     2701 tttgctcctt catcaggcct ggtagcgatg atcgttattg aatgcctgct gccatcgaat
     2761 tcgtcgtctt tccacagaag ttcccaggta gtctgataaa taatcatgga atttccattt
     2821 ttggtattac cagtcccgga ggcctgagat ttattttttc tgtaattaaa tatgacataa
     2881 gggggatccg gatctgaaac aatagcatat tgtattcgat aatggatagt gtcacggggt
     2941 attgagtaat tgtcaggaac gagataattg ataccgatct ggtagaagtc tccaatttca
     3001 ggggggtcat caaagtttat gacgtactca atcaaagctg tgtccctatt tgtctgccag
     3061 ttgtcgtcaa acgtattttg atatcgtttc tgtggagtat tatagtacca ttccggcatt
     3121 tcgcctgtaa aaaaacgcca gcttatgctg ttgatcttta ctgcgtttgg tttgatatcc
     3181 cttgccgtca cggtttcaaa accggggtaa ttgacttcaa ctgagtattc cctcccctct
     3241 gtaagtgcca gggaggactc tgcagggtaa accagatagc ctttattagt cattgttccg
     3301 gatgcaataa ccggggtgtc aaggattaat ctcttataaa acactccgtc ttcaaacatg
     3361 tcaactttac attcaatgtc catttgaata tgcggatgct gtatccctct cgccattgca
     3421 accctcacct cactgtcctt atccggaccg ataatggatt gaacggctaa gagaggttct
     3481 gtttcaggta agctgaaggt catatcacgg gagcatgagc agatacctcc gatgattatt
     3541 atcaaaccaa ttacccgtaa agctgtct
//
LOCUS       DAOPBK010000218         3562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_21145_length_3562_cov_3.293128, whole genome shotgun sequence.
ACCESSION   DAOPBK010000218 DAOPBK010000000
VERSION     DAOPBK010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21145_length_3562_cov_3.293128"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLA17_10265"
     CDS             <1..685
                     /locus_tag="PLA17_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK85837.1"
                     /translation="FGMAGDLGALDKIVPFKKKYKFRLLVDDAHGIGTMGRTGAGTGE
                     HFGVQDQIDVYFATFAKSFAGIGGFVAGDKEVISYLRFNLRSQIYAKSLPMAMTIGAI
                     KRLEMMKNNPELRERLWEITNALQKGFREAGFDLGRTQSPVTPVFFNGELEQTTYLIR
                     DLRRNYGIFCSVVVYPVVPRGVIMLRIIPTAVHTMEDVNYTIQAFKEVKKKLDANVYP
                     SDKIADFRN"
     gene            complement(766..1152)
                     /locus_tag="PLA17_10270"
     CDS             complement(766..1152)
                     /locus_tag="PLA17_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPK85838.1"
                     /translation="MRNFKKTFHINAEPSDIYAALTNPYTIELWSGYPAIMPSKAGEE
                     FNMWDGDICGRIIDLVPNRKVVQEWYFGDREVESHVTITINGNGGKTAVTVVHTNIPV
                     EDYQNIAQGWIEYYMGAISRFYNPNF"
     gene            complement(1190..2185)
                     /gene="trxB"
                     /locus_tag="PLA17_10275"
     CDS             complement(1190..2185)
                     /gene="trxB"
                     /locus_tag="PLA17_10275"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPK85839.1"
                     /translation="MQFFKPLETGSDEKKPATATEKARCLIIGSGPAGYTAGIYAARA
                     NLKPVLYTGLQQGGQLTTTTEVDNFPGYPDGVTGPVMMEDLRKQCERFGTEIRFGIAT
                     SSDFSGKPLKVTFDDSVEIEADTVIIATGATAKYLGIPGENKYAGMGVSACATCDGFF
                     YRGKDVAVVGGGDTACEEATYLAGLCRKVYMIVRRDVLRASKAMQQRVMNTPNIEVLW
                     KCQVEDLFGTGGVEGAVVVRGRGTPEEEKFSINIDGFFLAIGHTPNSDVFRPWVETDE
                     TGYIITDPGSTRTRVPGVFAAGDVADRVYRQAVTAAGSGCKAAIDAERYLAEQGH"
     gene            complement(2239..3042)
                     /locus_tag="PLA17_10280"
     CDS             complement(2239..3042)
                     /locus_tag="PLA17_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85840.1"
                     /translation="MSEMLSGKVLAVAAAVIITVSAGAQSVVVPRALAGMNGIESARS
                     RVFTETSLYGYINGGAELYLEYGFDTLLVTELVIEGSDIRAEVYRMKDHEAAFGIFSV
                     SRFRCNGGARLTEHLCRSAYQLQFCKGPYYVSIINDTGSDDDQKRSADLARLMIENIV
                     EPSFDPRSFYKDVLDDDTMRGAVLVRGPLGVYNGIPGLSEKLGRVSGYSALLIRHGTD
                     TIASMKFDSELAAGQFIREEMNGTGSGKSAGGSDSTVLVVSSRHIIITF"
     gene            complement(3129..>3562)
                     /locus_tag="PLA17_10285"
     CDS             complement(3129..>3562)
                     /locus_tag="PLA17_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPK85841.1"
                     /translation="NIPILKNAGMTVTLALKNLAFGSVSNTGRLHAKLWNETCAQVCA
                     FAPLRDKVVLNIVDGIKGCFNGGPGANPQFFCDYHTIITGSDPVAVDRIGFDVVIARR
                     IAEGLQKAPTPQGLEFMLQAEKLSLGVADREKIELREVILG"
BASE COUNT          842 a         1008 c          814 g          898 t
ORIGIN      
        1 cttcggcatg gctggtgacc tcggagcact tgacaagatt gtccccttca aaaagaaata
       61 caagttccgc ctcctcgttg atgatgccca cggcatcggc accatgggca gaaccggagc
      121 cggcaccggc gaacacttcg gcgttcagga ccagatagat gtctatttcg cgaccttcgc
      181 gaagagcttc gcaggaatag gcgggttcgt cgccggcgac aaggaagtga tcagctacct
      241 gagattcaac cttcgttcgc agatctatgc caagtcactt ccgatggcca tgactatcgg
      301 agccatcaag cgcctcgaga tgatgaagaa caatcctgag cttcgtgaga gactgtggga
      361 gattaccaac gctcttcaaa agggcttccg ggaggccggc tttgacctcg gccgcacaca
      421 gtcgcccgta accccggtct ttttcaatgg tgaactggaa cagacaacat accttatcag
      481 ggaccttcgc aggaactacg gcatcttctg ctccgttgta gtctatccgg tggtgcccag
      541 gggagtcata atgctcagga taatacctac tgcagttcac acgatggagg atgtcaatta
      601 tactatccag gccttcaagg aggtgaagaa aaagcttgat gccaacgtct acccgagtga
      661 taagatagca gacttcagga actgatccgc ctcacgctat tggggtatgc gggctggttt
      721 tctgatgaca ggcagaccgg aggatatggt tgatgttcta aagtgtcaga aattgggatt
      781 gtaaaaccgg cttatggcac ccatgtaata ctcaatccat ccttgtgcaa tattctggta
      841 gtcttccacc ggtatatttg tatgcacaac ggttactgcc gtttttcctc cgttgccatt
      901 tatggtgatt gtaacatgtg attcaacttc acggtcacca aaataccact cctgcacaac
      961 cttcctgttt ggcacaaggt caattatacg tccgcatata tccccgtccc acatattgaa
     1021 ctcctctcct gccttcgaag gcattatggc cgggtagccc gaccagagtt caattgtgta
     1081 cggatttgtc agtgcggcat aaatgtcaga tggctcagca tttatatgaa atgttttctt
     1141 gaaatttcgc atggcaggcg tttgtttccc ctttttatca attcattttt taatgtccct
     1201 gttccgcgag atatctctca gcgtcaattg ccgctttaca gccgcttccg gcagcagtga
     1261 cagcctgtct gtagacacgg tcagccacat cgcctgcggc gaacaccccc ggcacacgcg
     1321 tacgtgttga tcccggatcg gtgattatgt atcctgtctc atcggtttcc acccatggcc
     1381 tgaacacatc cgagtttggc gtatgcccta tggccaggaa gaatccgtca atgtttatgc
     1441 tgaacttctc ctcttcaggc gttcccctgc ctctgacaac aacagcgccc tcaacacccc
     1501 ctgttccgaa aaggtcctca acctggcatt tccacaggac ctctatattt ggtgtattca
     1561 tcaccctttg ctgcatggcc ttgctggccc tcagcacatc acgtcttaca atcatgtaaa
     1621 ccttccggca gaggcctgcc aggtatgttg cctcctcaca tgccgtgtca ccgcctccga
     1681 ccactgccac atcctttccg cggtagaaga atccgtcaca ggtggcgcag gctgaaacac
     1741 ccataccggc atatttgttt tcaccaggta tgccaagata cttcgccgtg gcgccggttg
     1801 caataatcac ggtatctgcc tctatctcca cgctgtcgtc aaaagtaacc ttcagcggct
     1861 tccccgagaa atctgatgat gttgctattc caaaccgtat ctccgtgccg aatctttcac
     1921 actgtttgcg gagatcttcc atcatcacgg ggccggtgac accatccggg taccccggga
     1981 agttgtcaac ctctgtggtg gtggtaagct gaccgccctg ctgcaacccg gtgtagagca
     2041 ccggtttcag gtttgcacgg gcagcatata tgcctgcagt gtatcctgcc ggcccggagc
     2101 caatgataag gcatcttgcc ttttcggtcg cagtggcagg cttcttctca tcactgcctg
     2161 tctcaagtgg tttgaaaaac tgcatattgc tattttctct gttaagacga caaaaatagc
     2221 aaataaatgt aaccaatatc aaaaagtgat aattatgtgt ctggaagaca ctactaaaac
     2281 agttgaatca ctgccacctg cgctctttcc ggaacctgtt ccgttcatct cctcacggat
     2341 gaactgccct gcagccagtt cgctgtcaaa cttcatcgat gcaatggtat ccgtgccatg
     2401 ccttatcagc agggcgctgt acccggaaac cctgccaagc ttttcagaca gtccgggaat
     2461 gccgttatag actccaagag ggcctctgac cagtaccgca cccctcattg tatcatcatc
     2521 gaggacatcc ttatagaaac tgcgagggtc aaatgatggt tccacaatat tttcaatcat
     2581 cagcctggcc agatcagcgg agcgtttctg atcatcgtcg ctgccggtat cattgattat
     2641 gctgacgtaa tatggcccct tgcagaattg caactggtaa gccgaacggc acaggtgctc
     2701 cgtcagcctg gcacctccgt tgcagcggaa acgcgagacg gagaatatgc caaaagcagc
     2761 ttcatggtcc ttcatccggt acacttcggc cctgatgtca ctgccttcaa tcacaagctc
     2821 agtaacaagc agcgtatcaa aaccatactc caggtacagc tctgctccac cgtttatgta
     2881 accatacaat gatgtctcgg taaaaaccct gctccgggcc gattcaatcc cattcatccc
     2941 ggcaagtgcg cggggtacga caacagactg tgccccggca gagaccgtga tgatgacggc
     3001 tgccgcaact gcaagcactt ttcctgaaag catctcagac ataaatatcc aatagtttta
     3061 tcccggtatc actatttttc cggtctcacc ttccatcccg gtctcaccat ttataccggt
     3121 attacctttt atcccaatat tacctccctc agctcgatct tctccctgtc ggcaacaccc
     3181 aatgacagct tctccgcctg cagcatgaat tccagtccct gcggtgtggg tgctttctga
     3241 agcccttcgg caatcctcct ggcgataaca acatcaaacc cgatacggtc aactgcaaca
     3301 ggatcggatc cggtaataat ggtgtgatag tcacagaaga actgcgggtt ggcaccagga
     3361 ccaccgttga aacatccctt gatgccgtca acgatgttca gaacaacctt gtcgcgcaga
     3421 ggcgcaaagg cacatacctg ggcgcatgtc tcgttccaca gcttcgcatg aagcctgccc
     3481 gtattagaga cagacccaaa ggcaaggttt ttaagcgcca gtgtcaccgt catgccggca
     3541 tttttaagta tggggatatt ga
//
LOCUS       DAOPBK010000219         3561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_21150_length_3561_cov_5.078437, whole genome shotgun sequence.
ACCESSION   DAOPBK010000219 DAOPBK010000000
VERSION     DAOPBK010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21150_length_3561_cov_5.078437"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLA17_10290"
     CDS             <1..346
                     /locus_tag="PLA17_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85842.1"
                     /translation="GNSGQANQNQNQNRSADNYNQGNQNQNQNQSAGNSGQANQKQNQ
                     SANQKKAANNSQAKVQGEGSSSSGVKKIQGARPDWSKARGARPASVERPSGSRIPKGA
                     GKPGGAKGPGKR"
     gene            369..1271
                     /locus_tag="PLA17_10295"
     CDS             369..1271
                     /locus_tag="PLA17_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85843.1"
                     /translation="MRRSRTCTYAIACCLLLQAVAVSAGAQPASVSGTHDTTGTDAKS
                     TIHSLYMGSGFGNNLLYLGTTMSQDCGFGYASVSYGLFDKLYLSAAGYTISNFKPFTA
                     FYSLGLGFNQTFNSWFDISLSLSRYNVAVSLRDTLFSNFTYADATLGFDWRILYSKIS
                     AGSLFAGESQFYLQTRHSRYFETPSFARDKAFFSFDPYVNILFGTMVNVTTSGGVTET
                     TISPGYKPWKGKGQGGGNPQPVTTYSESFGLMEIDFGIPVAFSYDFFTIEIEPGYVIP
                     FYSDEGTSGTSGFLLMASLFFRIF"
     gene            1281..1964
                     /locus_tag="PLA17_10300"
     CDS             1281..1964
                     /locus_tag="PLA17_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPK85844.1"
                     /translation="MNVLIVEDEKSLANEIALFLKRENFLCEMAHTGREASEKIAVNL
                     YDFVLLDLGLPDYNGLDLIREAKKNNSDVSFIIITARGAVEDRVKGLDLGADDYLPKP
                     FALAELHARIMAVTRRKFRISSPDIALGDFVMKTDSRKLMYGQKEVELTRKEFDLLYY
                     LAINRNKVLTRHQLYEHIWGNTLDDQYDSNFIDVHIKNIRKKLNAHAPAPWLETARGV
                     GYRISTDNQ"
     gene            1961..3301
                     /locus_tag="PLA17_10305"
     CDS             1961..3301
                     /locus_tag="PLA17_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPK85845.1"
                     /translation="MKKRGMMKLQTKLALFNLLSKLVFSALFIGFLPLIIERINVRQT
                     DKELVNKREEVIGLISEVGIEPFIHSDTAAGFGSYNILKEEYISLERSSTDDEWNYID
                     ITQRIIDDEVIDYRVLHYSLKIDGESYLLEIGMSLASITYAARNIRNVILIFLGLFIL
                     VTLLSDLTYTAQILRPLRKITSKLRKTSFSSFDVTEPLVTSTEDFARLDRALREMMKK
                     ISELFSKEKEITVNISHDLMTPVSVLRSRLENMLLNGNTDPATEAGIEESLRTLHRLK
                     VLVNSMLMIARLESHQYLKEESVGICDIVKEVTGELEPIAEDGKVTVTLTCETNFTMK
                     NANRSLLFSMLYNVVNNAVQHTPEGGMVDIHCGENSGRFEVSVSDTGKGMSPEHLDRL
                     FTRFSNRPDKSGNGTGIGLAITKSIADFHDIAIRVLSEPGKGTQFFFFFPLNSL"
     gene            3367..>3561
                     /locus_tag="PLA17_10310"
     CDS             3367..>3561
                     /locus_tag="PLA17_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85846.1"
                     /translation="MKKEMLLGAIALLISAGVMAQTTEPTQTQTRGDVGKEVREQARN
                     KGETQKSMNQARTASKNKGAS"
BASE COUNT         1015 a          864 c          854 g          828 t
ORIGIN      
        1 tggtaattct ggtcaggcca accagaatca gaaccagaac cggtctgcag ataattacaa
       61 tcagggcaac cagaatcaga accagaatca atctgctggt aattctggtc aggccaacca
      121 gaaacagaac cagtctgcaa accagaaaaa ggcagcaaat aacagccagg caaaagtaca
      181 gggagagggc agcagttcat ccggcgtaaa gaagatccag ggggcaaggc ctgactggag
      241 caaagcccgc ggagcccgtc cggcatcagt tgagaggcca tcgggttcac ggatacccaa
      301 gggagccggc aaacccggcg gagccaaagg acctggcaaa aggtaaataa actggtaatc
      361 atggggtcat gagacgaagc cggacatgta catacgcaat tgcctgctgc ctgttgcttc
      421 aggcagttgc agtatctgca ggggcacagc cggcatcagt ctccggcaca catgacacca
      481 caggtactga tgccaaaagc accattcatt cattgtacat gggctcaggc tttggcaaca
      541 atctgctcta cctgggtaca actatgtcgc aggattgcgg tttcggctat gcctcagtct
      601 catacggact gttcgacaaa ctttaccttt cagcagcagg atataccatc agcaacttca
      661 aaccattcac cgccttttac agcctgggac ttggtttcaa ccaaaccttc aactcctggt
      721 tcgacataag cctgagcctg tcaaggtata atgtggcagt ttcactgcgt gacaccctct
      781 tcagcaattt cacctatgct gatgcaaccc tgggattcga ctggaggatt ctctattcca
      841 agatatcagc cggcagcctg tttgccggtg aaagccagtt ctatcttcag acaaggcatt
      901 cgagatattt cgaaacccca tccttcgcca gggataaggc attcttctcg tttgacccct
      961 atgtgaatat cctcttcggc accatggtaa acgtcactac atcaggaggc gttacggaaa
     1021 ccaccatttc tccgggctac aagccatgga aaggaaaagg acagggaggt ggaaatcctc
     1081 agcctgtaac cacttacagt gaatcattcg gcctgatgga gattgacttc gggatccccg
     1141 tcgctttcag ctatgacttc ttcaccattg agatcgaacc cggttatgtc atccccttct
     1201 attccgatga gggaacttca ggtaccagcg gtttcctgct tatggccagc ctcttcttca
     1261 ggatttttta actttggatc atgaatgtcc ttatcgttga agacgaaaag agtcttgcaa
     1321 atgagattgc actcttcctg aaacgggaaa actttctttg tgaaatggcc catacgggaa
     1381 gggaggcatc ggaaaagatt gccgtcaacc tctacgactt cgtgctcctt gaccttggcc
     1441 tgcctgacta caatggcctt gacctcatca gggaggcaaa gaagaacaat tccgatgtct
     1501 cttttattat cattactgca cgtggcgcgg ttgaagacag ggttaagggc cttgacctcg
     1561 gggctgacga ctatcttccc aaaccatttg cactggcgga actgcacgca cggataatgg
     1621 cagtcacccg aagaaagttc cggatctcct ctcctgacat tgcccttggc gatttcgtta
     1681 tgaaaactga ttcaaggaag ctgatgtacg gacaaaagga ggtggagctg accaggaaag
     1741 agtttgacct gctctactac ctggcaatca acaggaacaa ggtgcttacc cgtcaccagc
     1801 tctacgaaca tatctgggga aacaccctcg atgaccagta tgacagtaat ttcatagacg
     1861 tacatattaa aaatatccgc aagaaactca atgcccatgc tcctgcgccc tggctggaga
     1921 cagcaagggg tgtgggatac aggataagca ctgacaatca atgaagaaga ggggcatgat
     1981 gaaactccag accaaacttg cgcttttcaa cctgctctcc aaactggtct tctcagccct
     2041 gtttatcgga ttcctcccgc tgataataga acgcatcaat gtcaggcaga ctgacaaaga
     2101 gctggtcaat aaaagagagg aagtgatagg tctcatctcg gaggtgggaa tcgaaccctt
     2161 tatccattct gacactgctg ccggtttcgg aagctataac atcctgaagg aggagtatat
     2221 aagcctggaa aggagctcaa ctgatgatga gtggaattac attgacatca ctcagagaat
     2281 aattgatgat gaggttattg actacagggt tcttcactat tccctgaaga ttgacggaga
     2341 gtcttatttg ctcgaaatcg gaatgagcct ggccagcata acctatgctg ccagaaacat
     2401 ccggaacgtg attctgattt tcctggggct tttcattctt gtcacgctcc tctctgacct
     2461 gacatatacg gcacagatac tcaggccact gagaaaaata acgagcaaac ttagaaagac
     2521 ttcattttcc tcatttgatg tcactgagcc actggtaaca tccaccgaag attttgccag
     2581 gcttgaccgc gccctcaggg agatgatgaa aaaaatcagc gagctgttca gtaaagagaa
     2641 agagataaca gtaaatatct cgcatgatct gatgacaccg gtgtcagtcc tccgcagcag
     2701 gcttgagaac atgctgctga acgggaacac agacccggcg acagaagcag gtattgaaga
     2761 gtctctccgc acgcttcacc ggctgaaggt gcttgtcaat tcaatgctta tgatcgcccg
     2821 gctcgagagc catcagtacc tgaaagagga aagcgtcgga atctgcgata ttgtaaagga
     2881 ggtcaccgga gaactggaac ccattgccga ggatggcaaa gtgacggtca cactcacatg
     2941 tgaaaccaat tttacgatga agaatgccaa caggtctcta ctcttctcca tgctgtataa
     3001 tgtagtaaac aatgctgtcc agcacacccc ggagggtgga atggtagata ttcattgcgg
     3061 tgaaaactca ggcagatttg aggtttcggt ttcagataca ggcaagggga tgtcaccgga
     3121 acacctcgac cggcttttta cgcgtttcag taaccggcct gacaaatccg gcaacggcac
     3181 aggaatcggc cttgccataa ccaaatctat tgctgatttt catgatattg caataagggt
     3241 tctgtcagag ccgggtaagg gaacacagtt tttcttcttt ttccccttaa attcattata
     3301 gcttcatttt caccggttta tttttggtct gttattatag gaatgaacta aaaaacagaa
     3361 caaaaaatga aaaaggaaat gctattgggc gctatagcac ttttgatcag cgcaggtgtc
     3421 atggcacaaa ctactgagcc aacccagaca cagacccggg gagatgttgg caaagaggta
     3481 agggaacagg ccaggaacaa gggggaaact cagaaatcaa tgaatcaggc aagaacagcc
     3541 tccaaaaaca aaggtgcttc a
//
LOCUS       DAOPBK010000220         3543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_21278_length_3543_cov_5.278956, whole genome shotgun sequence.
ACCESSION   DAOPBK010000220 DAOPBK010000000
VERSION     DAOPBK010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21278_length_3543_cov_5.278956"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..2071)
                     /locus_tag="PLA17_10315"
     CDS             complement(332..2071)
                     /locus_tag="PLA17_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPK85847.1"
                     /translation="MKKLFIIIAVFGLALSSCQDVLEDEYKNPETVNPPEEKSVAGMF
                     SGMLYQWQLFVKDYGEYWWQNDGWGIPAYAQIAHRYITDRYFAYYVDYEDVETGVNGF
                     HGSAVTNSFSYHYTKVKEWALMKEKIEAMSTAEQEQVEVYFKLATVFKDLLALRSVDF
                     FNSIPYSEALQGNVGKLYPKYDDPKEIYISILTDLKEISESLSDAYNKMNPDNKAYFA
                     VQDVALKGDISKWVQFINALRLQYGVKLAGVDQATGVPLMASALTNLPTQDLYFGPYS
                     YEYPSGGGTIIRGMYERTYANFIPNVIMKRMNHGTNTYEPGIDDPRLPVIAMPTKWNS
                     NQDYLGVSMNSDAQTPFYGAGHRYYPYGDNLESSLEQNAVSMYNFATYFHNFEKYPAI
                     MTTRAYVDLLLAEIALKNYGATGKTAAEHMRDAVIHSCDWWYWLNGVSSYRTDVAVLH
                     PVKSEADITTYAAKIADDFTAAVGEEEKMEIIMQQKYIHVNITDEYDLWAELRRTRHP
                     LLEPMVLRGEYMKPCPERLRYPSSELQLNGDNFKAVQDQDNYTSPIFWVPESKRGTVW
                     YLNDVLPLNLPPY"
     gene            complement(2092..>3543)
                     /locus_tag="PLA17_10320"
     CDS             complement(2092..>3543)
                     /locus_tag="PLA17_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPK85848.1"
                     /translation="SSYTQYSVLGQASFGWENTYYVEITARNDWSSLLPPANNSYFYP
                     GISATWNFSNTFSVPKLTYGKLRMSFADVGRAAPTPYWTNRDFGVGMVERTSAVTVYG
                     PSALFTSDIKNERKREFEIGFDTRWFEKRPLVAEFSFYTSHTYDQIMGLGITPATGFS
                     TIKINAGDVKNWGYELLLKYTPFKTDKLTWDVTLTTANQRTKVIKLYPGITEYRIAGS
                     NSYSIMAVEGKPIGDVQMYDYLRDSRGNRVVNSSGLYMRDNSKFVTAANINPDFIGGF
                     TTDLYYGKLSLHLGFDYKFGGTFLSHTNYYLLGNGQNKESLKYRDEANGGMAYYIDDA
                     TGKRIEWKHDMPAPAGARDNMVYHDGLILEGVKQVSDGVYEKNDIIITAYEYWTTYIQ
                     DMNEWFAPDKLYKNDYVKFREAAIMYTVPQQISNKLKLQKLTVSLVARNLFYIYKTMP
                     NIDPESLLGNDQFVEYTPLPQLRSYGFKIDVSF"
BASE COUNT          875 a          815 c          845 g         1008 t
ORIGIN      
        1 tctttgcttt acgatttcag ttgatatttg cttttttgtt gcccattcgt tgcccgaaat
       61 acataattaa ctgataatca gtattacttt acattttgta ttggcaaata gagttaggca
      121 aagacaacct cagtagggta aaagtcaatc aggttttgat gatgtttggg cacaggcatg
      181 tttcagattc tggaagcccg gttggatata ttaaaataaa catgaatgca aaagattaag
      241 tatcagtcat ttgttcctgt caggtaaaat aaagagggtg tcgtttcccg gaaatgacac
      301 cctctgaatt taatttaccc gtatccggtt tctagtatgg cggaaggtta agcggcagga
      361 catcattgag ataccatacc gtgcccctct ttgattcagg aacccagaag atgggagatg
      421 tataattgtc ctgatcctgt acagccttga aattatcacc gttgagctga agctctgagg
      481 atgggtaacg aagacgctca ggacagggct tcatgtattc gcctctgagt accatcggct
      541 caagcagcgg gtggcgtgta cgccgcaact cagcccacag gtcatactca tccgtgatgt
      601 ttacatgaat gtatttctgc tgcatgatta tctccatctt ctcctcctct cccactgctg
      661 cggtgaaatc atccgctatc tttgctgcat acgtggtgat atcagcctca gatttgacag
      721 ggtgcagaac ggcaacgtcc gtcctgtagg agctgactcc gttgagccag taccaccagt
      781 cgcatgaatg tatgaccgca tccctcatat gctctgctgc cgttttacct gttgcaccgt
      841 agttcttcag ggctatttca gcaagcagaa gatccacata tgcccttgta gtcattattg
      901 caggatactt ttcgaagttg tggaaatatg ttgcaaaatt gtacatcgaa acagcattct
      961 gttcaagtga gctctcgaga ttatctccgt agggataata cctgtgacct gcaccataga
     1021 atggagtctg agcatcagaa ttcattgaca cacccaggta atcctggttg ctgttccatt
     1081 ttgtcggcat tgcaatcacc ggtaatctgg ggtcatcaat acccggttca taggtatttg
     1141 tgccgtggtt catcctcttc ataatgacgt tggggatgaa gttggcatag gttctctcat
     1201 acattcccct gattattgtt ccgccaccgg agggatactc atatgagtaa ggcccgaagt
     1261 atagatcctg cgttggcagg ttggtcagag cgcttgccat aagtggcacc ccggttgcct
     1321 gatcaacacc ggcgagtttg acgccatact gcagcctgag tgcattgatg aactggaccc
     1381 acttggagat gtctcccttg agtgcaacat cctgcacggc gaagtatgcc ttgttatcag
     1441 gattcatttt gttataggca tcagaaaggc tttcggatat ctccttcaag tcagtcagaa
     1501 tggaaatgta tatctccttc ggatcatcat atttcggata gagttttccg acgttaccct
     1561 gcagcgcctc agaatacgga atgctgttga agaaatcaac gcttctgagt gcaagcagat
     1621 ccttgaaaac agtagcaagc ttaaagtaaa cttcaacctg ttcctgctct gccgttgaca
     1681 ttgcttcgat tttttccttc atcagggccc actccttgac cttggtgtaa tgataactga
     1741 aggagttggt aaccgcactg ccgtggaagc cgttaactcc ggtctccaca tcctcataat
     1801 caacataata agcaaagtag cggtcagtga tgtaccggtg tgctatctgg gcgtaggcag
     1861 gtatgcccca cccgtcgttc tgccaccagt attcgccgta atcctttacg aagagctgcc
     1921 actggtaaag catcccggaa aacattccgg caacagactt ctcttccggc gggtttacgg
     1981 tctcagggtt cttgtactcg tcctcaagca catcctggca tgatgacaga gccagaccga
     2041 agacggcaat gattatgaat agttttttca tgttatccat ttcttaattg gttagaaact
     2101 gacatcgatt ttgaaaccgt agctgcgaag ctgtggcaga ggagtatact ctacaaactg
     2161 gtcgttgccc agaagtgatt caggatcgat gtttggcata gtcttgtaga tatagaacag
     2221 gttcctggca acaagtgata ctgtcagttt ctgtagtttc agcttgtttg atatctgctg
     2281 tggaacggtg tacatgattg cagcctcacg gaacttgaca taatcattct tgtagagctt
     2341 gtctggagca aaccactcat tcatatcctg aatgtacgtg gtccagtatt cgtaagcagt
     2401 aataattata tcattctttt catagacacc atcagacacc tgtttgactc cttcgagtat
     2461 gagtccgtca tgatatacca tgttgtcacg cgcaccggcc ggagcaggca tgtcatgctt
     2521 ccattcaatg cgctttccgg ttgcatcgtc gatatagtat gccatcccgc cattggcttc
     2581 atccctgtat ttgagagact ccttattctg gccgttacca agcagatagt agtttgtgtg
     2641 cgagaggaag gtacctccga acttatagtc aaatccgaga tgcagactga gtttgccata
     2701 atagagatcg gtggtgaatc cgccgatgaa gtcgggattg atattggcag cagtgacaaa
     2761 cttcgagtta tcacgcatgt agaggccgct gctgttaacc accctgtttc cacgggagtc
     2821 ccgaagatag tcatacatct gcacatcacc gattggtttg ccttcaactg ccatgattga
     2881 ataactgttt gaaccggcaa tccggtattc tgttatgccg ggatagagtt tgatgacctt
     2941 cgtccgctgg tttgcggtgg tcagggtgac atcccatgta agcttatcag ttttgaaagg
     3001 agtatatttg agaaggagct cataacccca gttcttcacg tcacctgcgt tgatcttgat
     3061 tgtgctgaat ccggtggccg gggtgatgcc aagacccatg atctgatcat aggtatggga
     3121 ggtataaaag gagaactcag ctacaagagg tctcttctca aaccagcgtg tatcaaatcc
     3181 gatctcaaat tcgcgcttgc gctcattctt gatatcactc gtgaagagtg ccgatggacc
     3241 gtacacggtg acagcgctgg tacgctcaac cattcccacg ccgaagtcgc ggttggtcca
     3301 atagggtgtg ggagccgcac ggcccacatc tgcaaatgac attctcagct tgccgtaggt
     3361 gagctttgga acagagaagg tattgctgaa gttccatgtg gctgatatgc cgggatagaa
     3421 atatgaatta ttggccggcg gcagcagtga tgaccagtca ttccttgctg tgatttcaac
     3481 gtagtaggtg ttttcccatc cgaaggaggc ctgaccaagg acgctgtact gtgtatatga
     3541 act
//
LOCUS       DAOPBK010000221         3530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_21382_length_3530_cov_3.982734, whole genome shotgun sequence.
ACCESSION   DAOPBK010000221 DAOPBK010000000
VERSION     DAOPBK010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21382_length_3530_cov_3.982734"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="larB"
                     /locus_tag="PLA17_10325"
     CDS             <1..715
                     /gene="larB"
                     /locus_tag="PLA17_10325"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPK85849.1"
                     /translation="EIRAGNMSVEEAMEKLRDFPYTDLGFARIDNHRELRTGYPEIVY
                     CAGKTPEQVRAIFKAMERHNNNIIGTKAGKEMFDYISPSFPEAVYYETARIISIVKKE
                     IVPPASKIAIITAGTSDIPVAEEAAVTAELLGNEVIRIYDAGVAGIHRLVDKLPEIRS
                     CRVSVVIAGMEGALASVVGGLVNMPVIAVPTSVGYGAAFEGISALLAMMTSCAAGVTV
                     VNIDNGFGAGFAASRINRL"
     gene            721..1362
                     /locus_tag="PLA17_10330"
     CDS             721..1362
                     /locus_tag="PLA17_10330"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPK85850.1"
                     /translation="MADELLLSTAYFPPAGYMALIARSRGVLIEREENYLKQTWRNRC
                     LVMSANGPLPLIVPVLRGSFHKTAIRDLRIDNSRRWPELHLRGIVSGYSAAPFFEFYF
                     DMISGVVSRRHTFLLDLNSEALEAVCRATGIDVPVGYTDRFEQEGTRENDYRYRITPK
                     KASEIPGYRDLPYTQVFGDKQGFVAGLSIIDMLLNNGPGTRALLLRSLGADNC"
     gene            complement(1369..2883)
                     /locus_tag="PLA17_10335"
     CDS             complement(1369..2883)
                     /locus_tag="PLA17_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HPK85851.1"
                     /translation="MKRKILLITATVLMLPAWLAGQNMDDALRYSRLFYQGTARFNGM
                     SGAFTALGGDVSAIAINPAAAGVFRSMELTITPAVFFRDMNTDWNGYGSDDSYSGLTL
                     GHAGLVSSFRTGSGKGLTNLNFAYTFNRTNNYYRNAVIDGISNNGSMADFWALQGTGC
                     RTGELGGTAFMAWDTYLIDTLPNYLDEYASIFSYYGEIYPVYGQKVKRIIDNSGFSNE
                     HNFAIGASLGGKVYLGAGFGITNISYTGHYSHLESDDAQKVFDFVNFTYTDHFKASGS
                     GWNFKLGAIVRPVESLRLGVSFTTPAIYTINEDFYNSLSVKLDNDTPSDPSDDANPVS
                     GNENMTYSYRLTTPYHLNAGIAYQIGNFGLISADYEYIDYATARLSNGADGYNFDPEN
                     EDMAAEMGAAGNLRLGAEARFGPLYLRGGYSYHSPAFIKGTLNEEASSNGYSAGLGYR
                     QDNFYIDVAMVWLNSYEAYMMYPDDSRAVREYTSDPVNLNIKDKYLSVTVGLKF"
     gene            complement(2961..>3530)
                     /locus_tag="PLA17_10340"
     CDS             complement(2961..>3530)
                     /locus_tag="PLA17_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85852.1"
                     /translation="TQTPVRSETTTQTRTQQQTPVRTQTPVRTQTPVRTQTPVRTQTQ
                     SANRPEYQTAERSYTPSYNNPRVSSRPSYNTTRSNSGTTTQSSGTVYNRTTTAPRSSS
                     TTPAYNTPSRSSSSPVQYSAPSRSSSSSYSSGGRSSGSSFSSGSRSYSSGGSYSSGGS
                     YSSGSSSSSSSSSSSSSSSGGRSSSGGRR"
BASE COUNT          792 a          834 c          967 g          937 t
ORIGIN      
        1 ggagatcagg gccgggaata tgagtgttga agaggctatg gagaagctgc gtgactttcc
       61 ctatacagac ctgggatttg cacgcataga taaccaccgg gagctaagga cagggtaccc
      121 cgagatagtt tactgtgccg gcaagacccc ggagcaggtc agggccattt tcaaggccat
      181 ggagagacac aataataata taataggtac aaaagccggg aaggagatgt ttgactatat
      241 cagtccctct ttccctgaag cagtctatta tgagactgca cgtatcatca gcatagtgaa
      301 aaaggagatt gttcccccgg catcgaaaat tgcaatcatt actgccggca cctctgatat
      361 ccctgttgcc gaggaggctg ctgtcactgc cgagcttctc ggaaacgagg tgataagaat
      421 ctatgatgcc ggcgtggccg ggatacacag gctggttgac aagctgcctg agataaggag
      481 ttgcagggtc tctgtggtta ttgcaggcat ggagggagca ctcgcgagcg tggttggcgg
      541 actggtaaac atgccggtga tagccgtacc aaccagcgtg ggttacggag ccgcctttga
      601 aggtatctct gcgctgctgg ctatgatgac aagctgtgcg gcaggtgtta ccgtggtgaa
      661 catagacaac ggcttcgggg caggctttgc agccagccgt atcaacaggc tctgattgcc
      721 atggcagacg agctcctcct ttcaactgca tacttccctc ctgccgggta tatggctctg
      781 attgcccgca gcaggggtgt tctgatagag cgggaggaga attatctcaa acagacctgg
      841 cgcaacaggt gtcttgtaat gagtgcaaac ggtccgctgc ccctgattgt tccggtgctc
      901 cgcggatcat tccataagac agcgatcagg gatctgagga tagacaacag caggcgatgg
      961 ccggaactgc atctcagggg gattgtttcc ggctattcag ccgctccctt cttcgagttt
     1021 tactttgata tgatatcagg ggtggtgtca aggagacata cattccttct tgatctcaac
     1081 agtgaggcgc ttgaggcggt atgccgggcc acgggcattg atgtgccggt tggttacact
     1141 gaccgctttg agcaggaggg cacacgggaa aatgactaca ggtaccggat cactccaaaa
     1201 aaagcgtcag aaataccggg ttacagggat ctgccctata cacaggtatt cggtgacaag
     1261 caaggttttg ttgcggggct gagcataata gatatgctcc ttaataacgg cccagggacc
     1321 cgagcgttat tactaaggtc cctgggagca gacaactgtt aaccaacttt agaacttcag
     1381 tcccacagtg actgacaggt atttatcctt tatattcaga ttaaccggat cacttgtgta
     1441 ttccctgact gccctggagt catcggggta catcatgtat gcctcatagc tgttgagcca
     1501 caccattgcc acgtcgatgt aaaaattatc ctgtctgtat cccagccctg cgctgtatcc
     1561 gtttgatgat gcctcctcat tcagtgttcc tttgatgaat gccggactgt gatagctgta
     1621 tcctcccctc aggtagagtg gtccgaagcg tgcctcggct ccaagcctga ggttgccggc
     1681 agcgcccatc tcggcagcca tatcttcgtt ctccggatcg aagttatagc cgtcagcacc
     1741 attgctaagc cgtgccgtgg cataatcgat atactcatag tcagcgctga tcagtccgaa
     1801 gttacctatc tggtatgcga tacctgcatt caggtgataa ggggttgtga gacggtaact
     1861 gtatgtcatg ttttcattgc cactaaccgg gttggcatcg tcagaaggat cggacggtgt
     1921 gtcattgtca agcttgactg acaggctgtt atagaaatct tcattgattg tgtatatggc
     1981 aggtgtagtg aagctcaccc cgaggcggag tgattctaca ggtctgacaa ttgctcccag
     2041 cttgaagttc cagcctgaac ccgaggcttt gaaatggtcg gtataggtga aattcacaaa
     2101 gtcgaatacc ttctgtgcat catctgactc gagatggctg taatgacctg tatatgaaat
     2161 attggtgatg ccaaatccgg cacccagata aaccttgccg ccaaggcttg caccaatggc
     2221 aaagttatgc tcattgctga atcccgagtt gtcaatgatt ctctttacct tctgtccata
     2281 tacgggatat atctctccgt aataagaaaa gattgaggca tattcatcaa gataattagg
     2341 aagggtatca atgaggtatg tatcccatgc catgaatgcc gttccgccga gctcgccggt
     2401 cctgcaaccg gtaccctgca gggcccagaa gtcggccatt gaaccgttgt tgctgatacc
     2461 gtcaatcact gcattcctgt agtaattatt ggttctgttg aaggtgtagg caaagttcag
     2521 gttcgtgagt ccttttccac tgccagtacg gaatgatgag accaatccgg catggccgag
     2581 cgttagtccg gagtagctgt cgtcagagcc gtatccgttc cagtcggtat tcatgtcgcg
     2641 gaagaaaacc gctggtgtta tggttaattc cattgagcgg aatacgcctg ctgctgcagg
     2701 gtttattgca atcgccgaca cgtcgcctcc gagtgcggta aaagcgccgc tcataccgtt
     2761 gaatcttgcg gttccctggt agaaaagcct tgagtacctc agcgcatcat ccatgttctg
     2821 ccctgccagc caggccggaa gcatcagaac tgttgcggtt atcagtaata tttttctttt
     2881 catttccttt agttttaaat gtacacttcc tgttgctcct gcgatatgcg gaacatttat
     2941 cagcaatatt gtcccatatt ttaccttctt ccgcctgatg agctccgtcc tcctgatgaa
     3001 gaggaggatg aggatgaaga tgaagatgag gaagaggatc ctgagctata gcttccgccc
     3061 gaggagtatg agcctcctga gctgtagctt ctgctcccgg aagagaatga tgacccgctg
     3121 ctcctgccac cggaagagta tgatgaactg ctactgcgtg aaggagctga gtactgtacc
     3181 ggtgatgatg atgaccttga cggtgtgttg tacgccggag tagtactgct gcttcgcggg
     3241 gcagtggttg tcctgttgta tactgttccg ctgctctggg tagttgtacc gctgttgctg
     3301 cgggtagtgt tgtaggatgg tcttgagctt accctcgggt tgttgtagct cggggtgtag
     3361 cttctttcag cagtctggta ctcgggcctg tttgcagact gtgtctgtgt tctgaccggt
     3421 gtctgagtcc tgacaggtgt ttgtgttctg actggtgtct gtgtcctgac cggtgtctgc
     3481 tgctgtgttc tggtctgggt ggttgtttca ctcctcacag gagtctgagt
//
LOCUS       DAOPBK010000222         3522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_21438_length_3522_cov_4.267378, whole genome shotgun sequence.
ACCESSION   DAOPBK010000222 DAOPBK010000000
VERSION     DAOPBK010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21438_length_3522_cov_4.267378"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1005
                     /locus_tag="PLA17_10345"
     CDS             37..1005
                     /locus_tag="PLA17_10345"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPK85853.1"
                     /translation="MATEAFELPNMTAYTETCASVANVFWNHRLYSATGKAKYLDVLE
                     RTLYNGLISGIGEDGCNFFYPNPLESDGSFERAGWFECSCCPSNVARFMPSMKQYIWS
                     ETTAGINLNLFIGSSAHLSVPDGEADVTVETGYPWTGNVRVTVKLSAENSRFTMGVRI
                     PAWTGGAPAEGDLYRYVTPAKGRAGIKVNGKKFKAPISDGFAHIVREWKSGDIVEISL
                     PMEPRFIAARKEVAADSGRVALGIGPMVYCLEQADNGPVRELTVDTSVKPVFEFEQSL
                     TGGTGTLTFPVTDNTGTVKEARAVPYYSWANRGKGEMIVWIKTNNQ"
     gene            1101..3521
                     /locus_tag="PLA17_10350"
     CDS             1101..3521
                     /locus_tag="PLA17_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPK85854.1"
                     /translation="MMNIKGRVTIFAAAAAITMASCTGNKGDGNYPIRPVPFTSVKMT
                     DNFWAPRIKKNHEVTIPIAFGYCESTGRIKNFEIAGGLDTGAFQTIYPFDDSDVTKII
                     EGASYSLQTFPDAELEACLDTLIYKIGLAQEDDGYLYTNRTIAEMGYGKLHDWAEGER
                     WEKTNILSHELYNLGHMYEAAVAYYQATGKRELLDISIKSADLVDKDFGWDAFVSYPG
                     HQVIEMGLVKLYRVTGEKRYLDLAKFFLDARGARDDGEEYSQSHRKVIDQTEAVGHSV
                     RATYMYSGMADVAAILGDKAYINAITRIWEDLVYRKMYITGGIGASGGNEGFAEPYHL
                     PNMSAYCETCASIGDIFFNHRLFSLHGEGKYFDILEKTLYNGALSGVNLAGDRFFYPN
                     PLESNGQHERSAWFGCACCPSNVCRFLPAIPGYIYSVTDRDLYVNLFASNNADIEMGS
                     RSVRIAQETNYPWEGKVTMIIDPEKAGRFTVRVRIPGWARNEAIPGGLYSFGSQSDEK
                     HTLLVNGVPTDAKTENGYAVITRKWQAGDRIELDIPMPVRTVLADERVKEDTDKYAVQ
                     RGPLVFCAEWPDNAEGQVLGLVIDENPEFTATFRPDMLNGTEIITTKARLTSRNLDGT
                     VSLGDYRELTLIPYFLWNNRGPGEMMVWLPYKETSARPAPAPTIARKSKVTGSLQNRA
                     LASVTDQLEPANSNDHTWPYYHWWPNNNRWEWVRFDFTKPETVSKVKVYWFDDGPSGG
                     CRIPDAWEIEYLLGGTWRKVPSEAPLAVTRNAWNIAEIEPVKTTAIRINVKLNKDFSS
                     GIHEVIIE"
BASE COUNT          931 a          813 c          985 g          793 t
ORIGIN      
        1 ccggtggcag agtacaactg gtgattccag aaaacattgg ctaccgaagc cttcgagctc
       61 cccaatatga cagcatacac cgagacctgt gcttcggtag ccaatgtttt ctggaatcac
      121 cggttgtact ctgccaccgg caaggcaaag tatcttgatg tgctggaaag gactctctac
      181 aatggactta tatcgggtat cggagaggac gggtgcaact ttttctatcc caatcccctt
      241 gaatctgacg gaagttttga gagggcaggc tggttcgagt gctcatgctg cccctctaat
      301 gtggcccgtt tcatgccttc aatgaaacaa tatatatggt ctgagacaac ggccggcatt
      361 aacctgaacc tctttatcgg ttcatccgcg catctgtctg ttccggatgg tgaggctgat
      421 gttacggtag agaccggata cccatggacc ggcaatgtca gggtaacggt aaagctgtca
      481 gcggaaaata gccggtttac catgggtgtt cgtatcccgg cgtggaccgg aggagcacct
      541 gctgaaggcg atctgtacag gtatgtcaca ccggcgaagg gaagggcagg aataaaggtg
      601 aatggcaaaa agttcaaggc tccgatttct gacggattcg ctcatattgt cagggaatgg
      661 aagtcgggag acattgttga aatctccctt ccgatggaac cgagattcat tgcagcaagg
      721 aaagaggtgg ctgctgacag cggcagggtg gcactgggaa tcggaccgat ggtatattgc
      781 cttgagcagg ctgacaacgg acctgtcagg gagctgacag ttgacacttc ggtgaagccg
      841 gtatttgaat ttgagcagtc actgacaggc ggcacaggca ccctgacatt ccctgtaacc
      901 gacaacaccg gcacagtaaa agaggccagg gcagtgccat attactcctg ggcaaacagg
      961 gggaagggag agatgatagt ctggatcaaa acaaacaacc agtaaatcat gaaacccaca
     1021 tgtatcaaac agacaaaaat ctgtttatat gatcttcagt tgtgtgggtt gcataatgcc
     1081 tttaggatca aaattatatt atgatgaata tcaagggaag agttacaatt ttcgccgcag
     1141 cagcggcaat caccatggct tcatgcacag gaaacaaggg tgacgggaat taccccatcc
     1201 ggccggtgcc cttcacatcg gtgaagatga ctgacaactt ctgggctccg cgtatcaaaa
     1261 agaatcacga ggtgaccata cctattgctt tcgggtattg cgaatccaca ggacgaatta
     1321 aaaacttcga gattgccgga ggacttgaca ccggtgcgtt tcagacaatc tatccatttg
     1381 atgattctga cgttacgaag attatcgaag gagcaagcta ctcgctccag acctttccgg
     1441 atgcagagct ggaagcctgc cttgacacct taatatataa gataggcctt gcgcaggaag
     1501 atgacggtta cctctatacc aaccgcacga tagcggagat gggatacgga aagctgcatg
     1561 actgggcaga gggagagagg tgggagaaga ctaacatact gagtcatgaa ctttacaacc
     1621 tggggcacat gtacgaggca gccgtggcct actaccaggc aaccggcaag agggaattgc
     1681 tggatatttc tatcaaatcg gctgatcttg ttgacaagga cttcgggtgg gatgcatttg
     1741 taagctatcc cgggcaccag gtgattgaga tggggcttgt caagctctac agggttaccg
     1801 gggagaagag atatcttgac ctggcaaaat tcttcctcga tgcccgtggt gcacgtgatg
     1861 acggcgagga gtattcgcag agccacagga aggttattga ccagacagaa gcggttggtc
     1921 actcagtcag ggctacatac atgtattcag gaatggctga tgttgctgcc atccttggtg
     1981 ataaggcata tataaatgca ataaccagaa tatgggaaga cctggtttac aggaagatgt
     2041 acatcaccgg cggtattggc gcctctggcg gaaatgaagg ttttgccgag ccgtatcacc
     2101 tcccgaacat gtcagcatac tgcgagacct gtgcttctat aggagacatc ttcttcaatc
     2161 acaggctctt ctcgctgcac ggtgagggga agtattttga cattcttgag aagacgctct
     2221 ataacggtgc cctttcgggc gtaaacctgg caggagacag gttcttctat cccaacccgc
     2281 tggaatcgaa cggccagcat gagagatcag catggtttgg ctgcgcctgc tgtccgtcaa
     2341 atgtctgcag gttcctgcct gccattccgg gatacatcta ttccgtgact gacagggacc
     2401 tgtatgtaaa cctgttcgct tcaaacaatg ccgatattga aatggggagc cggagtgtga
     2461 ggatcgcgca ggagactaac tatccctggg aaggcaaagt caccatgatt attgatccgg
     2521 agaaggcagg caggttcacc gtgcgtgtcc gtatccccgg ctgggcaagg aatgaagcca
     2581 ttcccggcgg actgtacagc ttcggcagcc aatcggatga aaagcataca cttctggtca
     2641 atggcgtacc aaccgacgca aaaacagaga atggttatgc tgttattacc cgcaagtggc
     2701 aggcgggaga caggattgaa cttgatatcc cgatgccggt cagaactgtt ctcgctgatg
     2761 agagggtaaa ggaagataca gataagtatg ctgttcagag ggggcccctg gtgttctgtg
     2821 ccgaatggcc tgacaatgct gaaggccagg tgctggggct ggttattgac gagaacccgg
     2881 agttcactgc caccttcagg cctgacatgc tcaacgggac ggagattatt accacgaagg
     2941 caaggctgac gtcaaggaat cttgacggta cggtttcact cggagattac cgtgaactga
     3001 ctctgattcc gtatttcctg tggaacaacc gcggtccggg cgagatgatg gtatggcttc
     3061 cctataagga gacaagtgcc agacctgccc ctgcccctac gattgcccgt aagagtaagg
     3121 taaccggcag cttgcagaac agggctctgg catcagtcac tgaccagctg gagcctgcca
     3181 actcaaatga ccacacctgg ccctattatc actggtggcc gaacaataac cggtgggaat
     3241 gggtaaggtt tgacttcacg aagccggaga cggtatcaaa agtgaaggtt tactggtttg
     3301 atgacggccc gtctggcgga tgcaggattc ccgatgcatg ggagatagaa taccttttgg
     3361 gaggcacatg gagaaaggtt ccatctgaag cccccctggc ggtcaccagg aatgcctgga
     3421 acattgcgga aattgagccg gtaaagacca ctgccatcag aataaacgtg aaactcaaca
     3481 aggatttttc ctcgggaata catgaggtaa taattgagtg ac
//
LOCUS       DAOPBK010000223         3514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_21498_length_3514_cov_3.895056, whole genome shotgun sequence.
ACCESSION   DAOPBK010000223 DAOPBK010000000
VERSION     DAOPBK010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21498_length_3514_cov_3.895056"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..3287
                     /locus_tag="PLA17_10355"
     CDS             150..3287
                     /locus_tag="PLA17_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPK85855.1"
                     /translation="MIKKTQHPGILLKKYFGKLLMGFALMAMLMPAGTGTASASGLLP
                     DGLSSMQQRVITGTVAESDGTPLPAVSVREKGTTNGALTDAQGMYQITVTTPNPVLVF
                     SFVGYISQEISVGSQNVINVQLSSDEIGLDEIVVIGYGTVKKRDLTGSVASIGGEVMR
                     DVPAPNISQALQGRISGVDMSQTSTKPGAAMQIRIRGTRSLNASNDPLVVIDGIPFAG
                     SIGDINPGYIKSIDVLKDASATAIYGSRGANGVILITTNQGYAGQKAQVSYNSYVGLK
                     DVFAEYPMMSGPEFVALRAARGQYTNGVDESDNQDTNWQDLFYRTGIVMNHDLGVTGG
                     TANGNYNFSVGYYKDQAVIPGQNYTRFSMRGTLDQQVGKLFRFGFITNNNYSINDGNN
                     MGLYGVLSLSPIANPYEADGSWKRVIKMPLDDQWTYSREIIEGLGDTWIDRSKAYGSY
                     NTLFGELAIPGIQGLKYRINLGGNFRMSNGGSYTGEGVFSNNATTPSTAYVSNSLTTN
                     WAVENLLTYERTFAGRHQVNAVAMYSAEETMYNRSQMSARSIPADAFQYFNLGHAAGE
                     KTIDPNGQDYQVSGLMSWMGRAIYSYDNRYMLTATLRADASSRLAPGRKWHTYPAISA
                     GWNIKNESFMQNVTAIDMLKLRFGYGQTSNQSINPYQTLGRLSTRPYNFGSVFSTGYY
                     VSQLPNSNLGWEFSITWNYGLDISLLKNRISATIEYYVTNTKDILLSVNLPPTSGVSS
                     YMANIGETQNKGIEFSVDGVIIDSPGGFTWEAGFNLYANRNKLVALASGQERDEGNAW
                     FVGHPIDVIYDYEKIGLWQEGDPYLDILEPGGNVGMIKVKYTGEYNSDGTPVRQIGPD
                     DRQITSLEPNFMGGFNTRLSYKGLDLTAVGVFKNGGKLISTLYDASGYLNLMTGRRGN
                     VKVDYWTPTNTDAKYPNPAGVLSGDNPKYGNTLGLFDGSYLKIRTVTLGYNLANTSLI
                     RNAGIDRMRLYVTVQNPLVMFSPYHKESGMDPETNSYANENSATTGTYQRRLLTIGTN
                     TPSTRNYMIGIDLTF"
     gene            3300..>3514
                     /locus_tag="PLA17_10360"
     CDS             3300..>3514
                     /locus_tag="PLA17_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK85856.1"
                     /translation="MKSTYIKTVIGTVIMTLFLAACSDILEEQPRSIYEPGFFKTEKG
                     VQGGLTAMYAHLRYIYGQAYYYNACQTG"
BASE COUNT          973 a          823 c          859 g          859 t
ORIGIN      
        1 attaatacat cagaaaaact accagcatgc aagcagatga taatacctcg taaaacagag
       61 agactcctgc ctgaattatc agggaggagg cttaatcaac ttcaaccctc atataaacca
      121 ggaattttaa ccttgtaaac caaagtctta tgatcaaaaa aacccaacac ccaggtattc
      181 tgttaaagaa atacttcgga aaactactta tgggctttgc gctcatggca atgctgatgc
      241 ctgccggcac aggtactgca tcagcctccg gattactgcc tgacggtctc tcttcaatgc
      301 agcagcgtgt cataaccggc actgttgcgg agagtgacgg aactcctttg cctgcagtca
      361 gtgtcaggga gaaaggtaca acaaacgggg ctctgacgga tgctcaggga atgtatcaga
      421 ttactgttac aaccccgaac ccggtactgg tcttctcttt tgtgggctat atctcccagg
      481 agataagtgt cggctcacag aacgtgataa acgttcagct cagttctgat gagataggtc
      541 tcgatgaaat tgttgtaatt ggttacggta cggtaaaaaa gcgtgatctg acgggctcag
      601 tagcctccat cggcggtgag gtgatgcgtg acgtgcctgc cccgaacatc tcccaggctc
      661 tgcagggaag gatatcaggc gttgacatgt cacagacctc caccaagccg ggagctgcaa
      721 tgcagatccg tatccgtggc acccgctcac tgaatgccag caatgatccc ctggttgtga
      781 ttgacggtat accctttgca ggttctatcg gagatataaa tccgggctac atcaaaagca
      841 tagatgtact taaggatgca tctgcaaccg caatttatgg ttcccgtggt gcaaacggcg
      901 tcatactgat aacaaccaac cagggttatg cgggccagaa agcgcaggtg agctataaca
      961 gctacgtggg acttaaagat gttttcgccg aatacccgat gatgagcgga cctgaatttg
     1021 ttgccctgcg tgccgcccgt ggtcaatata ccaatggagt tgatgaatca gataaccagg
     1081 ataccaactg gcaggacctc ttctacagga caggtattgt tatgaatcat gacctgggag
     1141 ttaccggcgg tactgcaaat ggcaactaca acttctccgt cggctattac aaggatcagg
     1201 cggtcattcc aggtcagaac tacacacgtt tttcgatgcg tggaactctt gatcagcagg
     1261 ttggaaagct gttccgcttc ggttttatta ccaacaacaa ctactcaatc aacgatggga
     1321 acaacatggg tctctacgga gtgctcagcc tgtcgccgat tgcaaatccg tatgaagctg
     1381 acggctcctg gaaaagagtg attaagatgc cgcttgatga tcagtggaca tattcgaggg
     1441 agatcattga gggcctcgga gatacatgga ttgacaggag caaggcatac ggttcttaca
     1501 atacactctt tggcgagctg gccattccgg gtattcaggg gctgaaatac aggatcaacc
     1561 tcggaggaaa cttccgtatg agcaatggtg gaagctatac gggcgagggt gtgttttcaa
     1621 acaatgcaac cactccttca accgcatatg tttccaattc acttacaaca aactgggcag
     1681 ttgagaacct gcttacctat gaacgtacct tcgccggcag gcatcaggtt aatgccgttg
     1741 caatgtactc tgcagaggag acaatgtata accgttcaca aatgtctgcc aggagtattc
     1801 cggctgatgc tttccagtat ttcaatctcg gtcatgctgc cggagagaag acaattgacc
     1861 cgaacggcca ggattaccag gttagcgggc tcatgtcatg gatgggccgg gcaatatatt
     1921 catatgacaa caggtacatg ctgacagcca ctcttcgtgc tgacgcatca tcacggcttg
     1981 ctccggggcg caagtggcat acatatcctg caatatcagc cggatggaat atcaagaatg
     2041 agtcgttcat gcagaatgtt acggcaattg atatgctcaa gttacgtttc gggtatggac
     2101 agacttcaaa ccagtctatt aatccttatc agacccttgg ccgcctttct accaggccat
     2161 acaatttcgg ttcagttttt tcaacgggat actatgtgtc acagttgccc aactccaatc
     2221 ttggatggga attctcaatc acctggaact acgggcttga catatctctg ctgaaaaaca
     2281 ggatatcagc cacaatagag tattatgtaa caaatacaaa ggatatcctg ctcagcgtaa
     2341 acctgcctcc cacatccggg gtaagcagct acatggccaa catcggtgaa acacagaaca
     2401 agggtattga attttcggtt gacggtgtga ttattgattc gccaggaggc tttacctggg
     2461 aagccggatt caatttgtat gcaaaccgca ataagctggt tgcactggct tcaggccagg
     2521 aaagggatga aggcaacgcc tggtttgtag gccatccgat cgatgtgatc tatgattatg
     2581 agaagatcgg gctgtggcag gaaggtgatc catatcttga catccttgag ccgggcggca
     2641 atgtcgggat gattaaggtc aagtatacag gtgaatataa cagtgacgga actcctgtca
     2701 ggcagatcgg gcctgatgac cgtcagatta caagtctgga accaaacttc atgggaggct
     2761 ttaatacacg actctcctac aagggattgg atctgacagc tgttggtgta ttcaagaacg
     2821 gtggcaaact catcagtact ctctacgatg catcaggata tctcaacctg atgacaggcc
     2881 gcaggggcaa cgtaaaggtt gattactgga caccgacaaa cactgatgca aagtatccca
     2941 accctgcagg tgtactcagc ggtgataacc cgaagtatgg caatacactt ggcctgtttg
     3001 acggatcgta tctcaagata cgtaccgtta ctctcggtta taatcttgca aatacgagtc
     3061 tgatcaggaa tgccggtatt gacaggatgc gtctttacgt aactgtccag aatcctctgg
     3121 ttatgttctc accataccat aaggagtcgg gaatggaccc cgagacaaac tcctatgcta
     3181 atgaaaattc tgctactacc ggtacgtacc agcgccgtct gctgaccata ggtaccaata
     3241 ctccatcgac acgcaactac atgatcggta tagatttgac attctaaaaa ggaaaaatga
     3301 tgaaaagcac ttatataaaa actgttattg gaactgtcat catgacacta ttcctggctg
     3361 catgctctga tattctggaa gagcagcccc gcagtatcta tgagccaggg tttttcaaaa
     3421 ccgaaaaggg tgttcagggc ggactgactg ccatgtatgc gcacctcagg tacatatacg
     3481 gacaggctta ttactacaat gcctgtcaga ccgg
//
LOCUS       DAOPBK010000224         3496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_21627_length_3496_cov_5.159837, whole genome shotgun sequence.
ACCESSION   DAOPBK010000224 DAOPBK010000000
VERSION     DAOPBK010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21627_length_3496_cov_5.159837"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2255
                     /locus_tag="PLA17_10365"
     CDS             <1..2255
                     /locus_tag="PLA17_10365"
                     /inference="COORDINATES: protein motif:HMM:NF037222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glycosidase"
                     /protein_id="HPK85857.1"
                     /translation="GINYRAEIWFNGSLLSDTATDAMFLRRKYNITSLLNHDGHNRLA
                     VKVEPPLNPGKPNGGQGGDGTIGRDVTMQFTAGWDWIQPVRDRNTGIWDAVTIEITGD
                     IDIRNGFARTRVPGERLPGELQAPAFVTFSAELVNPTDRIVEGEIAVSYMGSTDRKKL
                     KLPPRSTVTFTFAEQKKSDPRIWWPNGMGQPAMYPAVIIFSDKKGNTLDREDLMFGFR
                     EAGSYFDDSTGARVFTINGQKLFIRGANWIASDGMMRLSPERYEAEVKMHAEMNMNMI
                     RVWGGSITERPEFYKACDRNGILVWQDLWITGDCNGRWPDTVRKADSQEVRRMYPDDH
                     ALFLRSVEDQVKMLRVHPSLYLVCGGNEFPPPANLDTLIRQKLEELDGTRIYLSESTS
                     PELSRNTIGGTADGPYNIREPLWFFSEKWHPFNPEIGSVGLPNIESLRKMMDEKDLVI
                     PAGSEINEVWRYHKYMGYGGMIEQLGEPTDLADFVRKAQLLNFEQYRALAEGYTSHMW
                     DWYTGFLVWKSQNPWPALKGQLYDWYLDQNAGFYGFRQGASPVHMMFNPVDSAIYVVN
                     TTPKERKGLRLEAKLSDENGRERWKKAEEMVVDGNSIVKVWEPDLAGSQAKVQFLNLR
                     ITYVSTGLQMDDNTYWFPKDGDRKNLTSLPEAKIVGQMMKNKDGKVSVDLANSGSVAA
                     FFLRMKVLRATDGEMLTPVFFDDNYLVLLPGEKRTIQIDMTGVSAEERNTPLLLHLEG
                     FNLQPAIIRL"
     gene            complement(2284..>3496)
                     /locus_tag="PLA17_10370"
     CDS             complement(2284..>3496)
                     /locus_tag="PLA17_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85858.1"
                     /translation="DLSLSFSDNYQGPHKFIVWPERSPTGRYVIKTSMGLSILWTPFF
                     LAGHVTAIISGADAGGYSPPYKFFLLVSALFFLATGLVCLRRILLQDASDTITAMVLA
                     AFVISTNLYWYTLYQGTMSHVYSFALISAFIWYSIKWHTNGERKAWPAMRLGLLIGMI
                     TLIRPTNIIIVLFFLFYGITPSGSLRQRGREMIADYRLLALMTLAALLVWTPQMIYWR
                     EMTGQWLYFSYGSNERFFFGNPAIIKGLFSWRKGLFIYTPLMVFAFVGIFILWRRSSP
                     HALPVTIFVPLNIYIIFSWWCWWYGGGFGQRAFIDSYALMAVATASLLSAGIGTVRKW
                     IRYPVMAAFLLFACLGVFYNVQYYHGAIHWDSMTREAYFDSFGRIKPSERFTQLLDEP
                     DYERAREGLDR"
BASE COUNT          910 a          807 c          992 g          787 t
ORIGIN      
        1 gggggataaa ctacagggct gagatatggt tcaacggcag cctcctcagt gacaccgcta
       61 ctgacgctat gtttctccgc aggaaataca atataacctc ccttctgaac catgacggtc
      121 ataaccggct ggcagtcaag gtagagccgc cgcttaatcc gggaaaaccc aacggcggac
      181 aggggggtga cggaaccatt ggccgtgatg tgaccatgca gtttacggca gggtgggatt
      241 ggattcagcc ggtcagggac aggaatacgg ggatatggga tgcagtgaca attgaaatca
      301 ctggtgatat tgacataaga aacggctttg cccgtacgag ggttcccggt gaacggctcc
      361 ccggcgagtt gcaggctcct gccttcgtga ccttctccgc cgagctggtg aatccgaccg
      421 acaggattgt ggaaggggag atagccgtgt catacatggg atctactgac agaaaaaagc
      481 tgaaactgcc gcctcgcagc accgtcacct ttaccttcgc ggaacagaaa aagagtgacc
      541 ccaggatttg gtggcccaac ggtatgggtc agccggcgat gtatcctgca gtaattatct
      601 tcagtgacaa aaaaggcaac acgcttgaca gggaggacct gatgttcggg ttccgtgaag
      661 cgggaagcta ctttgatgat tcaaccgggg cacgggtctt taccatcaac ggccagaaac
      721 tttttatccg cggtgccaac tggatagctt cagacggaat gatgcgtctc agtccggaac
      781 gttatgaggc tgaggtgaag atgcatgccg agatgaatat gaacatgatc agggtctggg
      841 gcggatcaat taccgagagg ccggagttct ataaagcctg tgacaggaac ggcatactgg
      901 tgtggcagga cctgtggatc accggcgact gcaacggaag gtggcctgac acggtcagga
      961 aggcggacag ccaggaggtc cggcgcatgt atcctgatga tcatgctctt ttccttaggt
     1021 ctgtagagga ccaggtgaag atgctcaggg ttcatccttc cctatatctt gtctgcgggg
     1081 gaaatgagtt cccgcctccg gcgaatcttg atactctgat ccgtcagaag cttgaggaac
     1141 ttgacggaac aaggatttac ctgagcgagt caacttctcc ggagctctcg cgaaacacca
     1201 tcggcgggac tgctgacggg ccgtataata tcagggaacc tctttggttc ttctctgaga
     1261 aatggcatcc gttcaatccg gagatagggt cagtggggtt gcccaatatc gagagtctca
     1321 ggaagatgat ggacgagaag gacctggtga ttcccgctgg cagcgaaata aatgaggtat
     1381 ggcggtatca taaatatatg ggctacggcg ggatgattga gcaattggga gaacccactg
     1441 acctggcaga tttcgtcagg aaggcacagt tgctgaactt tgagcagtac cgggctcttg
     1501 ctgagggata tacatcgcac atgtgggact ggtacacagg tttcctggtc tggaagagcc
     1561 agaatccgtg gccggcgctc aaggggcagc tttacgactg gtatcttgat cagaatgccg
     1621 gtttttacgg tttcaggcaa ggcgcctcgc cggtgcacat gatgttcaat cctgttgatt
     1681 cggcgatata tgttgtcaac accacgccca aagagcgcaa ggggctcagg cttgaggcaa
     1741 agctgagcga tgagaacggc agggagagat ggaagaaggc tgaggagatg gttgttgacg
     1801 gaaacagcat agtaaaggta tgggagcctg atctggctgg cagtcaggca aaggttcagt
     1861 tcctgaatct tcgcatcact tatgtctcca ccggtctcca gatggatgac aacacctact
     1921 ggttcccgaa ggatggtgac aggaagaatc tgacctcgtt gcctgaggca aaaatcgttg
     1981 gccagatgat gaagaacaag gatggcaagg tcagcgttga cctggccaac agcggaagcg
     2041 ttgctgcctt tttccttcgg atgaaggtgt tacgggccac tgacggcgag atgctgacac
     2101 cggttttctt tgatgacaat tatttggtgt tgttacccgg agaaaagcgc acgatacaga
     2161 ttgatatgac gggtgtcagc gcggaagaga ggaacacgcc cctgttgctg cacctggagg
     2221 gattcaatct gcagccggcg attataaggc tttaaaggtt aacggcaacc gctttttcat
     2281 agcttacctg tctaatccct cccgcgccct ttcatagtca ggttcatcaa gcagttgagt
     2341 gaaccgctct gagggtttta ttcttccgaa ggaatcaaaa tacgcctccc tggtcattga
     2401 atcccagtgg attgcgccgt ggtaatactg tacattataa aagacaccca ggcaggcaaa
     2461 gaggaggaat gctgccatca cagggtatct gatccatttt cttacagtac caataccggc
     2521 agacagaagt gatgcggttg ccacagccat cagggcatag ctgtcaatga atgccctctg
     2581 tccgaatcct ccgccgtacc accagcacca ccatgagaag attatgtaaa tgttaagggg
     2641 aacgaatatg gttaccggca gggcatgagg tgaacttcgt ctccagagga taaagatacc
     2701 cacaaaggcg aaaaccatca gaggggtata gataaagagt cccttgcgcc agctgaacag
     2761 gccttttatt attgccggat taccgaagaa gaatcgctca ttgctgccat atgagaagta
     2821 taaccattgt ccggtcatct ccctccagta tatcatctgt ggcgtccata ccaggagtgc
     2881 cgcgagggtc atcagggcaa gaagtctgta atcagcaatc atctccctgc ctctctgccg
     2941 gaggcttccg gacggggtaa tcccataaaa caggaaaaac agtacaatta tgatattggt
     3001 cgggcgtatc agtgtgatca taccaattaa aagtccaagc ctcatggcgg gccaggcctt
     3061 cctctctcca ttggtgtgcc atttgattga ataccatata aatgcactta tgagggcaaa
     3121 cgaatatacg tgtgacatcg tcccctggta gagagtgtac cagtataggt ttgtgctgat
     3181 aacgaatgca gccaggacca ttgcagtaat tgtatcagat gcatcctgca ggaggattct
     3241 tctcaggcag acaagtcctg tggcaaggaa aaagagtgcc gaaacaagaa ggaagaattt
     3301 atagggcgga gaatagcctc ctgcatctgc cccggagatt attgcagtaa catgacctgc
     3361 aagaaagaat ggagtccaga gaattgagag acccatactg gttttgatga catatctgcc
     3421 cgtggggctc cgctcgggcc agacaatgaa tttatgaggt ccctggtagt tgtcagagaa
     3481 gctcagtgag agatcg
//
LOCUS       DAOPBK010000225         3486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_21688_length_3486_cov_5.301661, whole genome shotgun sequence.
ACCESSION   DAOPBK010000225 DAOPBK010000000
VERSION     DAOPBK010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21688_length_3486_cov_5.301661"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..1932)
                     /locus_tag="PLA17_10375"
     CDS             complement(304..1932)
                     /locus_tag="PLA17_10375"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPK85859.1"
                     /translation="MKKTIIPVALIAMVTASCAGDNQLKKASETITPAELREYTRILS
                     ADSMMGRKPFTPGETITINYLAGELERTGFAPAFGDSWFQEVPMVEINTTVQSPVLIT
                     AAGRKLEFSVPDDIALKSPSMKEYVELVNIPMVFCGFGIVAPEYGWNDFSVVDVRGKC
                     AVVLVNDPGLYTGDSALFKGRTMTYYGRWRYKYEEAARQGATAILIIHETVGAGYDFS
                     IPRKSSILPGLYQEPAAGDPEPCPVTGWLSAGAASELFGAMGHDVEKLRGEACVRGFA
                     GFEMGASISLRLTNTHRRNSSMNVAGILRGTTRPEEAIVISAHWDHFGIGEKENGDSI
                     YNGAVDNSTSMAWALEIGEAFSSMKKRPQRSVILFFPTAEEQGLIGSSWFVANPPVKQ
                     ENLIACFNNDLLLPIGRMKDVMVTGYGQSELDDLLADAARKQDRYILPDPNPESGMYF
                     RSDHFPFARAGVPALFARGNCDSREYGREWAAEQENDFIRNRYHKPADNYYPEMNFDG
                     IAEDARAILDVAFTLVTSDVRPGWKPGSEFANIK"
     gene            complement(1929..3161)
                     /gene="pepT"
                     /locus_tag="PLA17_10380"
     CDS             complement(1929..3161)
                     /gene="pepT"
                     /locus_tag="PLA17_10380"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPK85860.1"
                     /translation="MQNLLERFLRYVRFETRPDDSNPSSPSSVSQMIFAEQLAYELEN
                     IGLSEVTLDGNGYLMATLPANNGSHGTVTGFIAHIDTSPEAPSANINPLVHHCYDGND
                     LLLNRERGIILSPDEFPELKRYIGQTLITTDGTTLLGADDKAGIAEIVTACEYLISNP
                     TLKHGKVRIAFTPDEEIGRGADKFDIKRFGADYAYTLDGGGIGELEYENFNAAAATVT
                     VKGRSVHPGTAKDTMINSVMVADVFDSLIPQDERPEHTAGYEGFFHLYDIRGSVEESV
                     MKYIIRDHDPVKFAARKKLLNEVAEATRIRYPGAEVNVVMRDQYFNMKEIIEANYHII
                     ETAIEAYRQAGLEPVIKPVRGGTDGARLSFMGLPCPNLFTGGHNYHGRFEYIPLQSME
                     KAVEVIINIITAENKSPK"
BASE COUNT          826 a          868 c          834 g          958 t
ORIGIN      
        1 gttggccagc cccgtaatca gaagcagatt attgcagttt atcgtgagta catcactcat
       61 caggtcagaa gcaaaagcgt gagttacctc acggtgaatc ttgtcaccac cgcaaaccac
      121 agtaccgttc aatatcgaac taatttcttc cagtttcatt tatctgctgt ttccaaatta
      181 catcattata aaagggtagc gcaaaggtat aaaaattgtg aaagcattca cagtgttatg
      241 aagataacaa taaaaatgca attcaatgag aatgctaaag gtatattatt gttaattaat
      301 gacttatttg atatttgcaa actcggaacc gggtttccat ccaggcctga catcacttgt
      361 cacaagagtg aaggcaacat caaggatagc cctggcatcc tctgcaatac cgtcaaagtt
      421 catttccggg taatagttat ctgcaggttt gtggtacctg ttcctgatga agtcattttc
      481 ctgctctgct gcccattccc tgccgtactc cctgctgtca cagttgcccc gggcgaagag
      541 agccggcact ccggcacggg caaatggaaa atggtcagaa cgaaagtaca tgccgctctc
      601 aggattggga tcaggcagta tgtacctgtc ctgcttccgg gctgcatctg cgagcagatc
      661 atccagttcc gactggccgt aaccggtcac catgacatcc ttcatccttc cgatggggag
      721 gaggaggtca ttattgaaac atgcaatcag gttttcctgt ttgaccggag gattggccac
      781 aaaccatgat gagccgatca gtccctgttc ttctgcagtt ggaaagaata ggattacaga
      841 cctttgcggt ctctttttca ttgaagagaa agcttcgcca atttcaaggg cccatgccat
      901 ggatgtgctg ttgtctaccg caccgttgta tatcgaatcg ccattctctt tctctccgat
      961 accgaaatgg tcccagtggg ctgaaattac aattgcttct tccgggcggg ttgttcccct
     1021 gaggattcct gccacgttca ttgagctgtt cctgcgatgg gtgttggtga ggcgcagtga
     1081 gatggaggca cccatctcaa agccggcgaa gcctctgaca caggcttcac cgcgcagttt
     1141 ctccacatca tggcccatgg ccccgaagag ttctgatgcg gcgccggcac taagccagcc
     1201 tgtgaccgga cagggctccg gatcaccggc agcaggttcc tggtatagtc ccggtaaaat
     1261 tgaacttttt cgtggtatgg agaagtcata gcctgcgccc acagtctcat ggatgatcag
     1321 aattgccgtc gccccctgtc tggctgcctc ctcgtatttg tatctccatc ttccgtaata
     1381 agtcattgtc cttcccttga agagggcaga gtcgccagta tagagtcccg ggtcatttac
     1441 cagaaccaca gcgcatttac ctctgacatc aacaaccgag aaatcattcc agccgtattc
     1501 aggtgccaca atgccgaatc cgcagaagac catcggtatg ttaaccagtt caacatactc
     1561 cttcattgaa ggcgatttca gggcgatatc atcaggtaca gagaactcca gtttcctgcc
     1621 ggcggcagtg atcagaaccg ggctctgtac cgttgtgttg atctccacca tcggaacctc
     1681 ctggaaccag ctgtcgccaa atgcaggagc gaatcctgtt ctttcgagct ctccggcaag
     1741 ataatttatg gtaattgtct caccgggggt aaagggtttg cgtcccatca tgctgtcagc
     1801 agaaagaatc cgggtatatt ccctcagttc agcgggagta attgtctcag aggctttttt
     1861 gagctgattg tctcctgcac atgatgcagt aaccatggct ataagagcta cgggaatgat
     1921 tgttttcttc attttggtga tttgttttct gcagtgatga tattgattat tacctccacg
     1981 gctttttcca ttgactgaag cgggatatat tcaaaccgtc cgtggtagtt atgtccgccg
     2041 gtgaacaggt tggggcatgg caggcccatg aatgaaagcc tggcaccgtc ggttccgccc
     2101 ctgacaggct tgatgaccgg ttctaatccg gcctgccgat aggcttcgat ggcagtctca
     2161 atgatgtgat agttggcctc aattatctct ttcatattaa aatactggtc acgcatgaca
     2221 acattcacct ctgctcccgg ataccttata cgtgttgctt cggcaacttc attcagcaat
     2281 ttctttctcg cggcaaattt aaccgggtca tggtccctga tgatatactt catgacactc
     2341 tcttcaacag atccccggat atcatagagg tgaaagaatc cctcgtaacc ggctgtatgt
     2401 tccggtctct catcctgcgg aatgaggctg tcgaagacgt cggccaccat aaccgaattg
     2461 atcattgtat cctttgccgt tccgggatgt acactgcgtc cctttacagt gaccgttgcc
     2521 gcagcagcat tgaagttttc atattccagt tctccaatgc ctccaccatc aagggtgtag
     2581 gcataatcgg ccccgaaccg ttttatgtcg aatttgtcag cccctcttcc gatctcctcg
     2641 tcgggtgtaa aggcaatcct gacctttccg tgtttgagtg taggatttga aatgagatac
     2701 tcacaggcgg ttactatctc agcgatacct gccttgtcat cagccccgag aagagtggtt
     2761 ccgtcagtgg ttatcagtgt ctgtccgata tatctcttca gttcaggaaa ctcatcgggg
     2821 gagaggatga ttcctctttc ccggttaagc agcagatcat ttccatcata gcagtgatgc
     2881 accagggggt taatgtttgc agacggggct tcaggagagg tgtcgatatg agcaataaat
     2941 ccggtaacag tgccatggga gccgttgttt gccgggagtg tggccataag gtatccgttg
     3001 ccgtcgagtg tcacttctga cagccctata ttctccagtt cataagccag ttgttcggca
     3061 aaaatcatct ggcttaccga tgagggtgag gaggggtttg aatcatcggg tcttgtctca
     3121 aacctgacat acctgagaaa tctttcaagg agattctgca tgagtggtta tttgattgag
     3181 ccgttaaaat tagtcattaa aaattaagaa acggcaaccc ttcgccatca tcattccggt
     3241 gaaattgaca gacatcaccc tgcctgccgt ggcgacggca acaaggagga acgccgcact
     3301 gctccggtcg ctatccacgg tgtgctcatg agccctgtat atttttcagg aaccgggcct
     3361 gtaatggccg ggcaggtcct gactgcgttg tcaggctcat ttgtaaattg tgttctccgg
     3421 cattttacac acctgcggga attaacttta ttatatttgt tagctcagaa gaatgattta
     3481 tactca
//
LOCUS       DAOPBK010000226         3427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_22131_length_3427_cov_4.219158, whole genome shotgun sequence.
ACCESSION   DAOPBK010000226 DAOPBK010000000
VERSION     DAOPBK010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22131_length_3427_cov_4.219158"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLA17_10385"
     CDS             <1..457
                     /locus_tag="PLA17_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylgalactosaminidase"
                     /protein_id="HPK85861.1"
                     /translation="RPYSRIHLVSGTKGCAQKWPTPAKIALGHSWLKREEQAALEAQY
                     TPEIVKRVGEMAKVIGGHGGMDFIMDWRLIDCLRNGLPLDQDVYDAALWSVITPLSEW
                     SVANRSSSIDVPDFTCGSWKANVPVDPNIPAGGNTMVIDIRGNSSQLEV"
     gene            538..2532
                     /locus_tag="PLA17_10390"
     CDS             538..2532
                     /locus_tag="PLA17_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPK85862.1"
                     /translation="MFGSTAFLIILVLSLLVYAVPEWLKHYFTLGLLTAGIALTSAWS
                     IGVLAGTGQEYEFPLFASEAGVRMVLVIDSLSAFFILVINFTVLIGFLYSRGYLEPYR
                     ARMNALRFSIHYFSYLWLWISMLMVVMVRDGLSFLIVWEIMALSSFFLVIFDAEERSI
                     LKTGISYLIMMHVSMFFILVAFLIVQKDTGQMSFDSLGEYFGRHRNLPLFLLFFAGFA
                     IKAGFIPLHTWLPEAHPPAPSHISGVMSGVMIKMGIYGIIRVVASMQSDLLVTGVIIL
                     IISLISGVLGVMMAIVQHDLKRLLAYHSIENIGIIGIGLGLGVIGQAIGNHALSLLGY
                     TGGVLHVLNHSLFKSLLFFNAGSVYLAAHTRNMEQTGGLMKRMPWTAILFLTGSLAIC
                     GLPPFNGFISEFLIYMGMFGSLPGASLFHSILIVGSIAALALIGGLAIFCFTKAFGIV
                     FLGEPRSEQARNAREADKSMIYPQLATAAMILFIGLGSPLVVKPVFGIVGQTWNLGTQ
                     PLVIGAFTSNLAQISLAGGIFVVTVAALLLYRRYHLSRRVVETGPTWGCGYTAVTAKH
                     QYTATSFAYNYNHLARPLLQTKKEIDEIGEDEIFPRKRKFLSHSDDLFKRHLVDRPVN
                     LVTGLLKRLAVMQTGEIRHYILYAFVFMLLVLLLATLKII"
     gene            2532..>3427
                     /locus_tag="PLA17_10395"
     CDS             2532..>3427
                     /locus_tag="PLA17_10395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPK85863.1"
                     /translation="MMAGFILIVVAALFFPGVILRTKSIASGRKGPGIFQPWKDIALL
                     LRKGTVFSDTTGLIFKIAPSITLATVIGAMLFLPFAQQKALLSFEGDFIMFAYLLALG
                     KFFTIIAALDTGSSFEGMGASREALFSMLVEPAFFILMATFAMFTGYTSFSVIFNHFF
                     ITGNDYVLIYSIIGAYLLVQITMIENSRLPVDDPKTHLELTMIHEVMILDYSGFDKAL
                     IHIAGYLKFAIYGALIVNLFIGMIPFQYAIPAFFSIQLLMAVAIGLIESFMARFRMSH
                     NPQFIVVLTSVSLLIFFGVLLIL"
BASE COUNT          772 a          808 c          849 g          998 t
ORIGIN      
        1 gaggccatat tcaaggatac atcttgtcag cggcaccaaa ggatgtgctc agaagtggcc
       61 gacaccggca aagatagccc ttggccattc atggttgaag cgtgaagaac aggctgcact
      121 tgaggctcag tatacacctg agattgttaa aagggtgggt gagatggcaa aggtaatagg
      181 tggtcacggc gggatggact tcattatgga ctggaggctt atagactgcc tgcggaacgg
      241 acttccgctt gaccaggatg tatatgacgc tgccctctgg agcgttatca cccctctcag
      301 cgaatggtcg gtggcaaaca ggtcgtcatc gattgatgta cctgacttta catgcgggtc
      361 atggaaggcc aacgttcctg ttgatcccaa catcccggca ggcggaaata caatggtgat
      421 agatatcagg ggcaacagca gccaactgga ggtataacgg gtcaattctg ccttaaggcg
      481 gcataaagta tgaccggata atatattttt taaatttgcg ggcaaattgc cggattaatg
      541 tttggttcaa cagcctttct gatcattctg gttctgtcac tgctggtcta tgcggtgcct
      601 gaatggttga agcattactt caccctgggc ctcctgacgg caggtattgc acttacctca
      661 gcatggagca ttggtgtgct tgcaggtacc gggcaggaat atgaattccc cttgtttgct
      721 tcggaagcag gtgtcaggat ggtactggtc attgacagcc tgtccgcctt ttttattctt
      781 gttatcaatt ttaccgtact gatcgggttc ctctactcca gggggtatct tgagccttac
      841 cgtgcccgga tgaacgcact ccgcttttca attcattact tctcatacct gtggctgtgg
      901 atttcaatgc tcatggttgt gatggtgcgt gacgggctct ctttcctgat agtgtgggag
      961 ataatggctc tctcatcctt tttcctcgtg atctttgacg cggaggagag gagtatattg
     1021 aagacaggca tcagctatct catcatgatg catgtgagca tgttcttcat tcttgttgca
     1081 tttctgattg ttcagaagga tacgggacag atgagctttg actcactcgg tgaatatttt
     1141 ggcagacacc gaaaccttcc gcttttcctg ctcttctttg ccggttttgc catcaaggcc
     1201 ggattcattc cgctgcacac ctggcttcct gaggcgcatc ctcctgcacc atcacatatc
     1261 tcaggggtga tgtcaggggt gatgatcaag atgggtattt acgggattat aagggtagtg
     1321 gcttcaatgc aatctgacct gctggttacg ggtgtcatta tcctgataat atcacttata
     1381 tcaggagtcc tgggcgtgat gatggctata gtccagcatg accttaaaag actgctcgcc
     1441 tatcacagca tcgagaatat aggcattatt ggaattggcc tcggccttgg tgtcatcggc
     1501 caggcaatcg gcaatcatgc tctttccctg cttggttata caggaggagt tctccacgtt
     1561 ctgaatcact ctcttttcaa gtcactgctc ttctttaatg ccggttctgt ttaccttgct
     1621 gcccatacgc gaaatatgga gcaaacggga ggtctgatga aacggatgcc atggaccgcc
     1681 attctgtttc ttaccgggtc actggctatc tgcggactgc caccgttcaa tggttttatc
     1741 tctgagttcc tgatatatat gggaatgttt ggcagcctcc ccggtgccag tctcttccac
     1801 tcaatactca ttgtggggtc aattgcagcg ttggcactca ttggcggact tgccatcttc
     1861 tgcttcacca aggctttcgg aatcgtcttt ctcggcgagc caaggtcaga acaggccagg
     1921 aatgcccgtg aggctgacaa atcaatgatc tatccgcaac tggccactgc agccatgatc
     1981 ctgtttatcg ggcttggttc gccgctggtg gtgaagccgg tttttggcat tgttggccag
     2041 acttggaacc tgggcacaca accactggtc atcggagctt ttacctcaaa ccttgctcag
     2101 atcagcctgg caggaggaat atttgtagtc actgttgccg cactgctgct ttaccgcagg
     2161 tatcacctta gccggcgtgt ggtggaaacc ggacccacct ggggttgcgg ctataccgcc
     2221 gtaacggcga aacatcagta tacggcaaca tcattcgctt acaactacaa ccaccttgcc
     2281 aggccattgc tgcagacgaa gaaggagatt gacgagatag gagaggatga gatattcccc
     2341 cgtaagagga aatttctttc gcacagcgat gacctattta aacggcacct ggtagacagg
     2401 ccggttaacc tggttacggg attgcttaaa aggttagccg tgatgcagac cggtgagata
     2461 agacattata ttctctatgc atttgttttc atgttgcttg tattgttact ggcaacactc
     2521 aaaataatct gatgatggca ggttttatac tcatagtggt tgcagctctc tttttccccg
     2581 gagtaatcct ccggacgaag agcatcgcct ccgggcgcaa gggcccgggt atattccagc
     2641 catggaagga tatcgctctc ctgctcagga aaggaactgt attcagtgac actaccggcc
     2701 tgattttcaa aatagctccg tcgatcaccc tggcaacagt cataggagcc atgctgtttc
     2761 tgccctttgc gcagcagaag gcattgcttt cctttgaggg cgattttatc atgttcgctt
     2821 acctgcttgc tctcggaaag ttctttacaa ttattgcagc ccttgatacc ggaagcagct
     2881 ttgaagggat gggcgccagc cgtgaagccc tcttctcaat gcttgttgaa cctgccttct
     2941 ttattctgat ggcaaccttt gcaatgttca ccggatacac ctcattttcc gtaatattta
     3001 accacttctt tattaccggg aatgattatg tattgatcta tagtatcata ggggcttatc
     3061 tgctggttca gattacaatg attgaaaaca gcaggctgcc tgttgatgat ccgaagacac
     3121 accttgagct tactatgatc cacgaggtga tgatacttga ttacagcgga tttgataagg
     3181 cgctgatcca tatagcgggt tacctgaaat ttgccattta cggagccctg atcgtcaatt
     3241 tgtttatcgg gatgattccc ttccaatatg ctatacctgc cttcttttcc attcaacttc
     3301 tgatggcagt agcaattggt ttgattgaat cttttatggc aaggtttaga atgagccata
     3361 atccacagtt tattgtcgta ctcacctccg ttagcctgct gattttcttt ggcgtattgt
     3421 taattct
//
LOCUS       DAOPBK010000227         3423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_22168_length_3423_cov_4.135689, whole genome shotgun sequence.
ACCESSION   DAOPBK010000227 DAOPBK010000000
VERSION     DAOPBK010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22168_length_3423_cov_4.135689"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="PLA17_10400"
     CDS             complement(<1..122)
                     /locus_tag="PLA17_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPK85864.1"
                     /translation="MKEKIIVTGGTGYIGSHTAVELITAGFDVVIIDNLYNSDIS"
     gene            complement(122..>1277)
                     /locus_tag="PLA17_10405"
     CDS             complement(122..>1277)
                     /locus_tag="PLA17_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPK85865.1"
                     /translation="LMKKGIDPSNELEHEAFEGTDIKFTADPQDLREASFHIIAVPTP
                     VTRHNLPDLEPVLKASETVGRILKKGDYVVYESTVYPGCTEEECIPVLERYSGLKCPS
                     DFKVGFSPERINPGDKNHTLTKIIKVTSGCDAESSENIAKTYEMIIEAGVHRTPSIKV
                     AEAAKVIENTQRDINIAFMNELSIIFNRMGINTYDVLEAAGTKWNFLKFYPGLVGGHC
                     IGVDPYYLSYKAKAMGYHPQMIDSGRFTNDSMGAYVGKETVKKLIAASKSPDRSRVLI
                     MGMTFKEDVTDIRNSKVIDVVNELRDFGVEVDVIDPGASQAEVKHEYGIDLKKGPEGK
                     YDAIIVAVAHKEYRNLDEKFFLDLLNEKGILVDLKGMYRNRMKWLTYWSL"
     assembly_gap    1278..1287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1288..1418)
                     /locus_tag="PLA17_10410"
     CDS             complement(<1288..1418)
                     /locus_tag="PLA17_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPK85866.1"
                     /translation="MYDKLMKREAKLSLIGLGYVGLPIALEFAKKISVVGFDIRPDR"
     gene            complement(1421..2371)
                     /locus_tag="PLA17_10415"
     CDS             complement(1421..2371)
                     /locus_tag="PLA17_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK85867.1"
                     /translation="MNNAPRNFGLIGIAGYIAERHLKAIKDTGNRLLASLDKFDCVGK
                     IDNYFPESDFFVEYERFDRHIDKLKRTGVQLDYVSICSPNYLHDSHIRFALRHQADAI
                     CEKPVVLNPWNIDALQEIEKETGRHIFNILQLRYHPVILNLRERIMALPKNRKHDIDL
                     TYITSRGNWYHISWKGDINKSGGIATNIGVHFFDMLGWIFGPVVSNRVHLLERDKASG
                     YLELENARVRWFLSIDWNDLPDEVKENGKRTYRHITVNNEELEFSEGFTDLHTITYRE
                     ILRGNGYGLEDARQGIEIVYQIRNSALSVLKDEYHPLIKK"
     gene            complement(2401..2979)
                     /locus_tag="PLA17_10420"
     CDS             complement(2401..2979)
                     /locus_tag="PLA17_10420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPK85868.1"
                     /translation="MEKEFFAHPTAVIDEGCTIGSGTKIWHFSHIMKNSVIGTNCNIG
                     QNVVVSPDVILGNNVKVQNNVSIYTGVICEDDVFLGPSMVFTNIINPRSAVIRRDQYV
                     TTMVRRGASIGANATIVCGNEIGQYSLIGAGAVITRPVKPYALVVGNPGRQTGWVSEY
                     GHKLSFDEGGLAVCPESGQQYRLSGGEVTRII"
     gene            complement(2985..>3423)
                     /locus_tag="PLA17_10425"
     CDS             complement(2985..>3423)
                     /locus_tag="PLA17_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK85869.1"
                     /translation="IGVNSRLDTIQAAILRVKLRHLDSFNEARARAAAFYDSQLSGLP
                     GITVPERSSFSSHIFHQYTLKIAGGERNALRECLENEGIPAMIYYPVPAHMQKAYLHL
                     GYRPDDFPVSAKLSGEVLSLPMHTELDDEQLAYITNCIRSKFI"
BASE COUNT          736 a          844 c          875 g          958 t
ORIGIN      
        1 gatatgtctg agttatacag gttgtcaatg ataaccacat cgaacccggc agttatcagc
       61 tcaacagcag tgtgtgaacc gatataacct gtaccgccag ttactattat cttctctttc
      121 atcacaggct ccagtaggtt aaccatttca tcctgttcct gtacataccc ttcaggtcaa
      181 caaggatacc cttttcattg agcaggtcaa ggaagaattt ctcgtcgagg ttgcggtact
      241 ccttatgagc cacagctaca atgatggcgt catattttcc ttccgggcct tttttaagat
      301 cgatgccata ttcgtgcttc acctcagcct gggatgctcc cgggtcaatg acatcaacct
      361 ccacgccgaa gtcgcgcagc tcattgacca catcaataac cttggagtta cgtatgtcag
      421 tgacatcttc cttgaaggtc atccccataa tcagcacgcg tgacctgtcg gggctcttgc
      481 tggcagcgat cagtttcttc actgtctcct ttccgacgta tgctcccatg ctgtcattgg
      541 tgaatctgcc ggagtcaatc atctgcgggt ggtagcccat tgccttggct ttgtacgaca
      601 gatagtaggg atctacgccg atacagtgac cgcccaccag cccggggtag aacttcagga
      661 agttccactt tgtgccggcc gcttcaagca catcgtatgt attgatgccc atcctgttga
      721 agatgatgga gagctcgttc atgaaggcga tgttgatgtc gcgctgggtg ttctcgatga
      781 ccttggctgc ttcggcaact ttgattgagg gggtgcggtg aacacctgcc tcaatgatca
      841 tttcgtatgt ctttgcaatg ttctcggatg attcggcatc gcagccgctg gtcaccttga
      901 tgatcttcgt cagtgtatgg ttcttgtctc ccgggtttat acgttcgggc gaaaagccca
      961 ccttgaagtc ggacgggcat ttaagccctg aatatctttc gagtacgggg atgcactctt
     1021 cctcggtgca gcccgggtaa acagttgatt catacacaac gtaatcgccc tttttcagta
     1081 tgcgcccaac ggtttcggag gccttcagca ccggttcaag gtcagggaga ttgtgcctgg
     1141 tcactggtgt ggggacagct attatgtgga atgacgcttc gcgaagatct tgcgggtcag
     1201 cggtaaattt gatatctgtt ccttcaaacg cctcgtgttc aagttcattg ctgggatcaa
     1261 tgcccttttt catcagtnnn nnnnnnnatg cggtcaggcc tgatgtcgaa gcccaccacg
     1321 ctgatctttt ttgcaaattc gagggcaatg ggaagcccga cgtaaccgag gcctatgaga
     1381 gagagctttg cttccctctt cataagttta tcatacataa tcatttttta attagtggat
     1441 gatattcatc tttcagcacg gaaagtgccg aatttcttat ctgatatact atttcaatgc
     1501 cctggcgtgc atcttccagt ccgtaaccgt ttccgcggag gatttcacgg taggtgatgg
     1561 tatggaggtc agtgaatcct tccgagaact ccagttcttc attattgaca gttatatgcc
     1621 ggtaggttct tttgccattt tccttcactt catctggcag gtcattccag tcaatgctga
     1681 ggaaccatct cacgcgtgcg ttttccagtt ccaggtatcc tgaggctttg tcacgttcga
     1741 gaaggtgtac cctgttggag accaccggtc cgaagatcca accgagcatg tcaaagaagt
     1801 ggacaccgat attggtggct attccgcccg atttgttgat gtcgcccttc catgatatgt
     1861 gataccagtt tccgcggctg gtgatatagg tgaggtcaat gtcatgcttc ctgtttttcg
     1921 gtaaggccat aatcctttca cgcaggttca atatcaccgg gtgatacctg agctgaagaa
     1981 tgttgaatat atgtcgtccg gtctccttct caatttcctg cagggcatct atgttccacg
     2041 ggttgagcac gacgggtttt tcacagatgg cgtcagcctg atggcgcaga gcgaacctga
     2101 tgtgcgagtc atgaaggtag ttgggtgagc agatgctgac gtaatcgagc tgcacaccgg
     2161 ttctcttcag tttgtcgatg tgtctgtcaa atctttcata ctcaacgaag aagtcgctct
     2221 cgggaaaata gttgtctatt tttcccacgc agtcaaattt gtcaaggctt gcgagcagcc
     2281 ggttgccggt atcctttatt gccttaagat ggcgttcggc tatatatccg gctatcccta
     2341 tgagcccgaa gttcctgggt gcattgttca taagattgtt tttcaaaggc ataataacag
     2401 ttaaataatt ctggtcacct cccctccgct aagcctgtat tgctgaccgc tctcagggca
     2461 tacggcaagg cctccttcat caaaggagag cttatgaccg tactctgata cccatccggt
     2521 ttggcgtccg gggtttccca ccacgagggc atatggctta acgggtctgg tgatcaccgc
     2581 tcctgccccg atgagggagt actgccctat ctcattgccg cacactatgg tggcattggc
     2641 gcctattgag gcgccgcgcc tgaccatggt ggtcacgtac tggtcacgcc tgatgacggc
     2701 gctgcggggg ttgattatgt tggtgaatac catagagggt cccaggaaaa catcatcctc
     2761 acatataact ccggtataga tggatacatt gttctgtacc ttgacattat ttccgagaat
     2821 cacatccgga gagacaacca cgttctggcc aatgttgcag ttggtgccaa tgacggagtt
     2881 tttcatgata tggctgaagt gccagatttt cgtcccgctg ccgatagtgc agccttcgtc
     2941 tattacagcg gtggggtggg caaaaaattc cttttccata ttattcagat gaattttgag
     3001 cggatacagt tggttatgta agcaagctgt tcgtcgtcaa gctccgtatg catcggcagc
     3061 gagagcacct cgccggagag ctttgcggag acggggaagt catcaggcct gtaacccagg
     3121 tgcaggtatg ctttctgcat gtgagcgggc accgggtaat agatcattgc cggtatgccc
     3181 tcgttctcaa gacactcccg gagggcgttg cgctcgccgc cggctatctt gagagtgtac
     3241 tggtggaaga tatgtgatga gaatgatgat ctttcaggca cggtgatgcc gggcagtcct
     3301 gacagctgtg aatcatagaa ggctgccgcc ctggcacgtg cctcgttgaa cgagtcaaga
     3361 tgtcgcagct tgaccctgag gatagccgcc tggatagtat ccagacggga gttgaccccg
     3421 atg
//
LOCUS       DAOPBK010000228         3411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_22248_length_3411_cov_5.123659, whole genome shotgun sequence.
ACCESSION   DAOPBK010000228 DAOPBK010000000
VERSION     DAOPBK010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22248_length_3411_cov_5.123659"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PLA17_10430"
     CDS             <1..616
                     /locus_tag="PLA17_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HPK85870.1"
                     /translation="GHQTYLTDQQVVNRFNRRHYDMMRIGEVRSAVHETEPLFRDASA
                     ALFDIGSARQSDAPGTFLPSVNGFYGEEICLLARYAGLSDNLKLFSVTEVNPTLDTRM
                     QTSNLAAQLIWFFLEGFSQKQYEVSYLSDLTNSRFTRYHVTLSDLESEAIFIKSTITE
                     RWWMEIRDIHDKPQYLACSYEDYLMANRDQVPVRWSRGMLRHGH"
     gene            700..1644
                     /locus_tag="PLA17_10435"
     CDS             700..1644
                     /locus_tag="PLA17_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPK85871.1"
                     /translation="MKKTRIFILALLLPAGIVCGQQDPAFSHYMFNTQTFNPGFSGMS
                     GMITASALTRQQWVGFPGAPSTMLFNINMPFSLFGFRSGAGLLVEADKYGFSNDVNIT
                     LSYSGLFSVGTGTLGAGFSIGVLNKTVAPEWFIPSGPGHTPPSGDPLIPENDESFLAP
                     DFSAGIYYQGLNYYAGLSVTHLNQPKIKYSQTATYVSRQYYFTAGYYFQLPNPAFELV
                     PSIFIVNDGATTQYLATGMVKYNKKIWGGVSYRISDAITGFAGIELYNGLKIGYGYDF
                     PISEIRKGTSGSHEFVVSYSFDIGLGKSVTKYKSIRFL"
     gene            1690..3024
                     /locus_tag="PLA17_10440"
     CDS             1690..3024
                     /locus_tag="PLA17_10440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPK85872.1"
                     /translation="MKKVTLLALALILMLSSCGSYESGELTGVQGRKKFYEPEPFEMA
                     FIPAGSFVQGPSDQDPLRAMNSLSKTVTVEAFWMDQTEITNNKYRQFTYWVRDSILRT
                     RLGEEGIEGYEFFRTDKEGNVLEPNVLNWSEKIDLDDENIQAVMETMYYPPEERFNGN
                     KQWDTRLLNYSYFWVDYKQAAKARYNYKEQRYEGTVTDLEGNTTEVKDRSSFIMHHVV
                     NIYPDTLAWIRDFTYSFNEPWATLYFWHPGYDDYPVVGISWIQARAFSVWRTNFMNEH
                     LRAQGIADVHNYRLPLETEWEYAARGGLDLSMYPWGGPYTRNYQGCFLANFKPLRGNY
                     IDDGAVYTIKVASFEPNEYGLFDMAGNVSEWTSCAYHPSSYIFTHDLNPDYEYNARPD
                     DPPVLKRKVIRGGSWKDIALFLQTSTRDYEYQDSTKSFVGFRNVRTYIGVNN"
     gene            3052..>3411
                     /gene="gldL"
                     /locus_tag="PLA17_10445"
     CDS             3052..>3411
                     /gene="gldL"
                     /locus_tag="PLA17_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HPK85873.1"
                     /translation="MGLSEFVHTSKWKAFMSKLYGWGASVVIIGALFKIQHYPMAGLF
                     LSVGLITEAIIFFFSAFEPLKEEPDWKLVYPQLDPNYSGEDLGFAYVGGGGGGFGGGG
                     GVGGGGGAGLTQFDKMLS"
BASE COUNT          861 a          876 c          835 g          839 t
ORIGIN      
        1 agggcatcag acctatctca ctgaccagca ggtggtgaac aggttcaacc gccgtcatta
       61 tgacatgatg cgcattggag aggtgcgatc tgcggtgcat gagaccgagc cactcttccg
      121 tgacgcctcc gccgcactgt ttgatatcgg atcagcaagg cagtcagatg cccccggaac
      181 attccttccg tcagtcaacg ggttctacgg tgaggagata tgcctccttg cccggtacgc
      241 aggcctgtca gataacctga agcttttttc cgtgacggag gtcaacccaa ctcttgacac
      301 caggatgcag acatcgaacc tggcagcaca gctcatctgg ttcttcctcg aaggcttctc
      361 gcagaaacag tatgaggtct cctatctcag tgacctgacg aacagcaggt tcacccgtta
      421 tcatgtgacc ctcagtgacc tggagagtga agccatcttc ataaaaagca ccattaccga
      481 gcgctggtgg atggagatcc gtgacatcca tgacaaacca cagtatctcg cctgctctta
      541 tgaagattac ctgatggcta accgtgatca ggtacccgtc aggtggtcac gcggaatgct
      601 ccgtcatggg cactgatttt tggcctcaat tttgtggcat gaatatattt ttatttattt
      661 tacccgttat atgtatgggt ttacaggcac tacccggcta tgaagaaaac aaggatattc
      721 atcctggcat tattactccc ggcaggtatc gtctgcggtc agcaggatcc ggcgttcagc
      781 cactatatgt tcaacaccca gacatttaat cccgggtttt cgggcatgtc ggggatgatc
      841 actgcttcgg cccttacgcg ccagcagtgg gtgggtttcc ccggggcgcc ctccaccatg
      901 cttttcaaca tcaatatgcc tttcagcctc ttcggcttcc gcagtggcgc aggcctgctt
      961 gtggaggctg acaaatacgg gttcagcaat gatgtcaata tcacgctttc ctattcaggc
     1021 ctcttttcgg tcggaaccgg taccctcgga gccggtttca gcataggggt gctcaacaag
     1081 actgttgcgc cggaatggtt catcccttcg ggcccgggcc atacacctcc ttcaggtgac
     1141 ccgctgattc ccgagaatga tgaatctttt cttgcacctg acttttccgc cgggatatac
     1201 taccagggac ttaactacta tgccgggctg tcagtcacac acctgaacca gccaaaaatc
     1261 aaatattccc agacagctac ttacgtcagc cgccagtact acttcactgc aggttactac
     1321 ttccagcttc caaaccctgc ctttgagctt gtcccctcaa tttttatagt aaatgacggc
     1381 gccacaacac aatatttagc cacaggtatg gttaaataca ataaaaagat atggggtggc
     1441 gtttcttaca ggattagtga cgcaatcaca ggattcgcag gcatagaatt atacaacggt
     1501 ctaaagatag gatatggata tgacttcccg atatcggaga tcagaaaagg aaccagcggc
     1561 tcccacgagt tcgttgtctc ttacagcttc gacatcggtc tcgggaagag tgttaccaaa
     1621 tacaagagta tccgtttcct ttaatagaca ggaaaaaata gaattacttg catatttaaa
     1681 tttaagtata tgaaaaaggt aaccctttta gctcttgcgt tgattttaat gttaagcagc
     1741 tgtggctctt acgaatcagg cgagttgaca ggcgtgcagg gaaggaagaa gttttatgag
     1801 ccggagccgt ttgaaatggc attcataccg gccggttcct ttgtacaggg gcccagtgac
     1861 caggaccctt tgcgcgccat gaacagcctc tccaaaacag ttacggtaga agcgttttgg
     1921 atggaccaga cagagattac caataataaa tatcgtcagt tcacctactg ggtgcgtgac
     1981 tccatcctca gaaccaggct gggagaggaa ggaattgagg gttacgaatt cttcagaact
     2041 gataaggagg gcaatgtcct ggaacccaac gtgctgaact ggtcagagaa gattgacctg
     2101 gatgatgaga acatccaggc tgtgatggaa acaatgtatt atccccccga agagagattc
     2161 aacgggaaca agcagtggga cacccggctc ctgaactact catatttctg ggttgactac
     2221 aagcaggcag ccaaagccag atacaactac aaggagcaga ggtacgaggg taccgtaacc
     2281 gaccttgagg gcaataccac cgaagtgaaa gaccgttcat cgttcattat gcatcacgtg
     2341 gtgaatatct accctgatac ccttgcatgg atcagagact ttacctattc attcaatgag
     2401 ccgtgggcaa cactctactt ctggcatccc ggttatgatg actacccggt tgttggaata
     2461 tcatggatcc aggcacgtgc attcagcgtg tggcgtacca acttcatgaa tgagcacctg
     2521 cgtgcacagg gcatcgccga cgtgcacaac taccgtctcc cgctggagac cgagtgggaa
     2581 tatgcagcac gcggcggact tgacctctcc atgtacccat ggggcggccc ctacacacgc
     2641 aactaccagg gatgcttcct cgcaaacttc aaaccactgc gcggtaacta catcgatgac
     2701 ggagccgttt acacaataaa ggtggcctcc ttcgaaccca atgaatacgg actgttcgat
     2761 atggcaggaa acgtgtccga atggacttcg tgcgcctacc atccttcatc atatatcttt
     2821 acccatgacc tcaaccccga ctatgaatac aacgcccgcc cggacgatcc gcccgtgctc
     2881 aagcgcaagg taatcagggg aggctcatgg aaagatattg cattgttcct tcagacatct
     2941 acaagggact atgaatacca ggactcaaca aaatcatttg tcgggttccg caatgtgaga
     3001 acttatatcg gagttaataa ctaataccat ctaactttaa tacataatta tatgggcctc
     3061 tcggaatttg ttcataccag taaatggaaa gcattcatgt ccaagcttta cggctgggga
     3121 gcctcagtcg tgatcatcgg tgcgctcttc aagatacagc actatccgat ggctggacta
     3181 ttcctctctg tgggtcttat cacagaagca atcatcttct tcttttcggc gtttgagccg
     3241 ctcaaggaag agcctgactg gaagcttgtc tacccccagc ttgacccgaa ttactctggc
     3301 gaggatctcg gttttgcata tgtcggcggt ggcggcggtg gattcggcgg tggcggtggc
     3361 gtcggcggtg gcggtggagc cggccttacg cagtttgaca agatgttgtc t
//
LOCUS       DAOPBK010000229         3355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_22679_length_3355_cov_5.179697, whole genome shotgun sequence.
ACCESSION   DAOPBK010000229 DAOPBK010000000
VERSION     DAOPBK010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22679_length_3355_cov_5.179697"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLA17_10450"
     CDS             complement(<1..698)
                     /locus_tag="PLA17_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPK85874.1"
                     /translation="MNCIIVDDDPVWLRTLEEFVGKSSSLTLVGSYSDSLSARNLLMS
                     RSDIELIFLDIEMPEMDGFDFLSSLENPPHIIFVSGSEEYAYKAFNYNGIDYLLKPIT
                     YPRFCRAVEKIMKYYAPRSVPSSAGEEEIFIKKGSSLVRLKLKEILFVEALENYVSVN
                     TRDEKYTIHFTMRGIEQQLPSSLFVRIHRSYLVNKSVIRSITENSLTLAIGGNTKILP
                     VGKSYRDGIMNYIN"
     gene            complement(854..1126)
                     /locus_tag="PLA17_10455"
     CDS             complement(854..1126)
                     /locus_tag="PLA17_10455"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPK85875.1"
                     /translation="MTKKELVSSIAKKAGLSLKDANIALNAFISSFGEAMKKGQRLQL
                     PSMGTFKVEKRSARVVRNPRTGAKLNVPAKKVVKFKAAPALLKELK"
     gene            1458..1544
                     /locus_tag="PLA17_10460"
     tRNA            1458..1544
                     /locus_tag="PLA17_10460"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1492..1494,aa:Ser,seq:tga)
     gene            1777..2646
                     /locus_tag="PLA17_10465"
     CDS             1777..2646
                     /locus_tag="PLA17_10465"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPK85876.1"
                     /translation="MTTDITLEVTGQTTLMPFLISSLKKKSRDNIKSLLRNRQVLVNG
                     EPVTQFNHELKPGDSITISATRHTTGVMARNMRLVYEDEHLVVIDKNAGLLSMASDNE
                     KYLTAYNILSTYVKSQKPSNRIFIVHRLDRDTSGLMMFARSKKVQSLLQRNWKQNVTS
                     RTYVALVEGEVTEPEGVIKSYIYESRSLVMHSTRNPEKGDLAITRFRRIKSGKDYSLL
                     EVTLETGKKNQIRLHMQELGHSIAGDRKYGASSSPIGRLGLHASVLAFVHPVTGKEMR
                     FESKIPAKFRRMV"
     gene            complement(2870..>3355)
                     /locus_tag="PLA17_10470"
     CDS             complement(2870..>3355)
                     /locus_tag="PLA17_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK85877.1"
                     /translation="VSQQAILFNDTFYNNISFAFDEIRDEESVVRAATIANAHAFITE
                     TPEGYQYIVGEGGNKLSGGQRQRISIARAIMANPPILILDEATSALDTESERLVQDAM
                     IKLMENRTSVVIAHRLSTIQHADMIVVVEDGRIEECGTHEELIRNPDGIYRKLYEMQS
                     F"
BASE COUNT          921 a          825 c          793 g          816 t
ORIGIN      
        1 atattaatgt agttcattat cccgtcgcgg tatgacttgc ccacaggcag aatcttcgta
       61 ttgccgccga tagctaatgt aaggctgttc tcggtgatcg acctgataac gcttttgttt
      121 acgaggtacg aacggtggat tctcacgaag aggcttgagg gcaactgctg ttctatgccc
      181 ctcatggtaa agtggatggt atatttctcg tcccgtgtat tgacactgac ataattctca
      241 agagcctcaa caaagagtat ctccttgagc ttcaaacgca caagcgaaga acccttttta
      301 atgaagatct cctcctcacc tgcagatgac ggcacagacc tgggtgcata gtatttcatg
      361 attttttcca ccgcacggca gaaacgcggg taggtaatcg gtttgagaag gtagtctatg
      421 ccgttgtagt taaaagcctt gtaggcatat tcttcgctac ccgaaacaaa tatgatatgc
      481 ggaggatttt cgaggctcga caggaaatcg aagccatcca tctccggcat ctcgatatcg
      541 agaaagatga gttctatatc acttcgtgac atcagcaggt ttcgtgctga cagagaatca
      601 gagtaacttc ccacgagagt aagggaagaa gacttaccta cgaattcctc gagggtccgg
      661 agccatacag ggtcatcatc aacaataata caattcattc ccggaacagg tagttaggtt
      721 tgagcgttgc aaacaaatat actaaaataa ttgacaaaaa aattgccccg gtatttaatg
      781 aacacaaaaa aaacgggccg gccggtatca ggaaaagaaa ataaaaaggg gtgcctgcgc
      841 acccctgaaa gttttacttg agttctttta aaagagccgg agctgctttg aatttcacga
      901 ctttctttgc gggaacattc agtttggcgc cggtgcgggg attgcgaaca actcttgcac
      961 ttctcttttc aactttgaaa gtacccattg aggggagctg gagcctttgg ccttttttca
     1021 tggcctcacc aaatgagctt atgaaagcat tcagagcaat gttggcatct ttaagagaaa
     1081 gccctgcttt ttttgcaatg ctacttacca gttctttttt tgtcatagtc agtacaattt
     1141 agggttagac caaattttct aattgcctac aaatttattt cattcttatt caaaagcaag
     1201 tttttataac cttttttatg cctgaaaggc cggtttttat gatatttgag cactttttcc
     1261 gttcataccc cccggtaaga tggtgaaaag agactcgttg atggtgtcag gcacccattt
     1321 acaggggagt tttgagaagc agagaaggga gccggataag ctttccggtt aaatttttac
     1381 caccgggcag aaaagcaaaa aaagattata tttgcacccg ctgcgcccgt cacccacggt
     1441 gagtaaggga tgcaaaggga gaaatggcag agtggtcgat tgcggcggtc ttgaaaaccg
     1501 ttgtccgggc gactggaccg ggggttcgaa tccctctttc tccgcagacc gtagcactcc
     1561 tgttccgtca ctgacagaac aggtttttta ttttacggca taccggaccg ggctcgcccg
     1621 ggggtgaggg atgacggaaa ataaaacaac cctcatgcac cgcccggtgc atgagggagt
     1681 gctatggtct gcaggttccc ccgccaggga tcacaagcga atgaaatgag cgggtaatcc
     1741 cacattctcc cctatctttg catcaatacc cggaatatga caactgacat cactctcgag
     1801 gttaccggac agacaaccct gatgcccttc ctcatcagct cgctgaaaaa gaagagccgc
     1861 gacaatatca agtcactcct gcgaaaccgc caggtactgg tcaacggcga gccggtgaca
     1921 cagttcaacc atgaactgaa accgggtgac tcaataacca tctccgccac aaggcacacc
     1981 acaggagtga tggcacgcaa catgcgcctg gtctatgagg atgagcacct ggtggtgatt
     2041 gacaaaaacg cggggcttct ctcaatggct tcggataatg aaaagtatct caccgcctat
     2101 aatatactca gcacatacgt aaagagccaa aaaccgtcaa acaggatatt catcgtccac
     2161 cgtctcgacc gcgacacctc ggggctgatg atgttcgcac gaagcaaaaa ggtgcagagc
     2221 ctccttcagc gcaactggaa acagaacgtc acttcaagaa cctatgtcgc cctcgtcgag
     2281 ggcgaggtga ccgaaccgga aggggtcata aaatcatata tatatgagag caggtcactg
     2341 gtaatgcact caacacgcaa tccggaaaaa ggtgatctgg caatcacaag attcagaaga
     2401 ataaaaagcg gaaaggatta ctccctcctt gaggtgacac tcgaaacggg aaaaaagaac
     2461 cagataaggc ttcacatgca ggagctgggc cacagtatag ccggcgacag aaagtacggc
     2521 gcctcctcaa gccctatagg aaggctggga ctccatgcca gcgtgctggc attcgttcac
     2581 cccgttaccg ggaaggagat gcgattcgaa tcgaagatac ctgcaaaatt cagacgtatg
     2641 gtataaaccg gctgaaaact tctgctcctt tgacgacagc atcaacccac gaagttcaga
     2701 cgtatggtat aaaccggctg aaagggccgg aagttattgt cataacaggg ctgctccctc
     2761 aactccttgc tgatgagcgc cagcagaata tgccaacatc ccacccacag ataccgaaga
     2821 gcatctggtg gcactctttg cgcgtcagca gaatatgtca gcatcccacc tagaacgact
     2881 gcatctcgta cagtttcctg tagataccat ccggattcct gatgagttcc tcgtgtgttc
     2941 cgcactcctc tatcctgcca tcttccacta ccactatcat gtctgcatgc tgtatggtgg
     3001 aaaggcggtg ggcgattact accgaagtgc ggttttccat cagcttaatc atggcatcct
     3061 gcaccagccg ctctgactca gtatcgaggg ccgaggtggc ctcatcaagg ataaggatcg
     3121 gcggattggc catgatggct cttgcaatgc tgattctctg gcgctgacct ccacttagtt
     3181 tgttgccccc ctcgccaaca atatactggt aaccctcagg tgtctcagtt atgaatgcgt
     3241 gagcattggc tattgtggca gcccgcacaa cactctcctc atccctgatt tcatcaaagg
     3301 caaatgatat attattataa aaggtatcat tgaacagtat tgcctgctga ctcac
//
LOCUS       DAOPBK010000230         3333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_22865_length_3333_cov_3.760220, whole genome shotgun sequence.
ACCESSION   DAOPBK010000230 DAOPBK010000000
VERSION     DAOPBK010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22865_length_3333_cov_3.760220"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1286
                     /gene="nusB"
                     /locus_tag="PLA17_10475"
     CDS             267..1286
                     /gene="nusB"
                     /locus_tag="PLA17_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPK85878.1"
                     /translation="MISRRQLRIKALTVLYSCNRKEISDLETAEQELMLSIRRSYDLY
                     HYLLLLLISLSDLAREKVALSGMKKIPGPEDINPNRRFADNRVIDQLRKNRQLRSYSE
                     ATPALWTRKDKLNSMKALNRWIATKGLSWNDYPEVVRIIFNEMIQWEVYKAYMSSLEN
                     SYKADLRFIKELVTKFFPASQDLETSLEEQSVYWNDDLPFAASMIRNSLSRFRENDEG
                     SAISIPPLFTNEDDEKYVKELLRKTILHSEKNSELIDNHTTNWEVERIALMDKLVMQL
                     AITELVEFPEVPVKVTLNEYIEIAKEYSTAKSSTFVNGILDKIVRELRAGGTINKSGR
                     GLLGE"
     gene            1300..1737
                     /locus_tag="PLA17_10480"
     CDS             1300..1737
                     /locus_tag="PLA17_10480"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPK85879.1"
                     /translation="MKNALTYLMAVLVTAALLTGACGRNRPGAGNIAPSDTTGTAVLT
                     FAAPEHNFGTVMEGEKVGCIFTYTNTGDADLVISSASASCGCTVPKFDKKPVPPGRSG
                     TIEVIFDTSGREGVQTKTVVVQSNAENNLVILRIIADVTKKSR"
     gene            1786..2112
                     /gene="yajC"
                     /locus_tag="PLA17_10485"
     CDS             1786..2112
                     /gene="yajC"
                     /locus_tag="PLA17_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPK85880.1"
                     /translation="MTTSAFIYLQAAPAQGSPNIMTGLLPLILVFVVFYFFMIRPQMR
                     KQKELTNYRNAIAKGDRIITTGGIYGKVVEVAANIVTVEIANDVKIRVDKNAILKDQS
                     DAAARQ"
     gene            2137..2907
                     /locus_tag="PLA17_10490"
     CDS             2137..2907
                     /locus_tag="PLA17_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HPK85881.1"
                     /translation="MNDKKERKARFLNRELLVFAIFLLLSFALWYLNELSKELQGSIN
                     YPVRYINPPKDRIVTGDLPDKLEMDLRGPGYSILKVKLSGSRAPVVIDFTKVSPRRIP
                     GLLPRYYLVTSGLMSSFSKQLHADFDILAIHPDTLFFGYDKLVTRKMPVIPNMKVELA
                     EGRRMIIVTDPDSITVTGPQHVLDTLDGIPTKYRIFRRMNENFRSRIPIECPEYLQTS
                     QKRVNVEVTVIGRAVSSQNGNGEAVRHQSLTGEQRPGS"
     gene            2924..>3333
                     /gene="coaE"
                     /locus_tag="PLA17_10495"
     CDS             2924..>3333
                     /gene="coaE"
                     /locus_tag="PLA17_10495"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPK85882.1"
                     /translation="MAKRLGVTGGIGSGKTTVCRIFRVLGIPVFVADNVAKDVMDNEP
                     DIRAAINLITGKDLYAHGKLNRKELARIIFNKPDLLRKVNAAVHPEVLERFKRWASAS
                     AAPYVIMEAAILFEAGADSLVDRVVTVSAPVEER"
BASE COUNT          956 a          726 c          836 g          815 t
ORIGIN      
        1 tcaaaaaagt atgtcctctt acctgccctg acaacttttg taaacacctc ctctttctca
       61 cttttctcat tctgaccttc aattcttcct ttttcgtctt ccataagtag cgaatgtttt
      121 agggtttcaa tcggttagca tggtcaaatg taacaattat tttttcaact caaagtaggg
      181 ctgacaaaat gtgatacttt tttggcccgg aggtggcgtg ctatgctgat ttttttaact
      241 ttgctgcctg aaaaaatgtt ctttaagtga taagcagaag acaactcagg atcaaggcgc
      301 tgactgtgct atattcctgc aacaggaaag agatatccga tctcgaaact gccgagcagg
      361 agctcatgtt aagtatcagg cgaagctatg atctgtatca ttatctgctt cttctgctca
      421 tctccctctc tgatctggca cgggaaaagg tggcactatc aggcatgaag aagattcccg
      481 ggccggaaga cattaatcca aacaggaggt ttgcagacaa cagggttatt gaccagctga
      541 ggaaaaacag gcagcttcgc agctattccg aagcgacgcc cgcattgtgg acccgcaagg
      601 acaagcttaa cagcatgaag gctctgaacc gctggattgc gacaaaaggg ctttcgtgga
      661 atgactatcc ggaagtggtc aggatcatct tcaatgagat gatacaatgg gaggtctaca
      721 aggcttacat gagctcgctc gaaaacagtt acaaggctga cctgcgcttc atcaaagagc
      781 tggtaacgaa gttttttcct gcttcgcagg accttgaaac cagtctcgag gagcagtcag
      841 tctattggaa tgacgacctg ccctttgcag cttccatgat acgcaactca ctgagcaggt
      901 tcagggaaaa tgacgagggc agcgcgatat caatccctcc gctctttacc aatgaggatg
      961 atgaaaaata tgtaaaggaa ttgctcagaa agacgatcct ccacagtgag aaaaacagtg
     1021 agctgatcga taaccatacc actaactggg aagtggagcg gattgccctg atggataagc
     1081 ttgtaatgca attagctatt actgaactgg ttgagtttcc agaggtgcct gtgaaggtta
     1141 cactgaatga atatattgaa attgccaaag agtacagcac tgccaaaagc agtacttttg
     1201 tgaacggaat ccttgacaag atcgttaggg agttgagagc aggcggaacc ataaacaagt
     1261 ccggcagagg gctccttggc gaatagaaaa attaaaggaa tgaaaaatgc tttgacatat
     1321 ctgatggcag ttctggtgac tgcagcactt ctgaccggcg catgcggaag aaacagacca
     1381 ggggccggca acattgcacc ctcagacacc acgggtacgg ccgtgctgac ttttgctgcc
     1441 ccggaacaca acttcggaac agtgatggaa ggagagaagg tgggatgtat ctttacatat
     1501 accaataccg gcgatgctga ccttgttatc agctcagcct ctgcaagctg cggttgtacg
     1561 gtgcccaaat ttgacaaaaa acccgtgcca ccaggcagat cgggcaccat agaggtgatc
     1621 ttcgatacat ccggcaggga gggagttcag acaaaaactg ttgtagttca gtcaaatgca
     1681 gagaataatt tagttatttt gcgtattatt gccgatgtga cgaagaaaag cagataacag
     1741 ataaatatta atcaatacaa ataatatcaa acaaagagat cacacatgac aacatcagca
     1801 ttcatttatt tgcaggcagc tcctgcccag ggaagtccaa acataatgac cggccttctt
     1861 ccgcttatcc tggtatttgt ggttttttat tttttcatga taaggccgca gatgaggaag
     1921 cagaaggaac tgacaaacta ccgcaatgca atagccaagg gtgacaggat aataacaacc
     1981 ggtggcattt acggcaaggt tgtggaggta gctgccaaca ttgtgacagt agagattgcc
     2041 aatgacgtaa agatcagggt tgataagaac gccatcctca aggatcagtc agacgctgct
     2101 gcaaggcaat aaaacaaaag gatatacatt gcaaccatga atgacaaaaa ggaaagaaag
     2161 gcaagatttt tgaacaggga gctccttgtt tttgcgattt tcctgttact ttcctttgca
     2221 ttatggtatc tcaacgagct cagcaaggag ttgcagggtt caattaatta cccggtaagg
     2281 tatatcaatc cgcccaagga ccggattgtg accggagatc ttcctgacaa gcttgagatg
     2341 gatcttcgcg gaccgggcta ttccattctg aaggtaaagt tgtcaggcag cagggccccg
     2401 gtggtgattg actttaccaa ggtctcaccg cgaaggatac ccggtctttt accccgatac
     2461 tatcttgtta cctcagggct catgagcagt ttctcaaagc agttgcatgc tgattttgac
     2521 atccttgcca tccatccgga tacactcttt tttggttatg acaagctggt gacgagaaag
     2581 atgcccgtca ttccgaatat gaaggtggag cttgcggaag gaaggaggat gattattgtg
     2641 acagaccctg acagcattac tgtcacgggg ccccagcatg ttctggatac ccttgatggc
     2701 atacctacaa agtaccggat attcaggcgg atgaatgaaa acttcaggtc gcggattccg
     2761 attgaatgcc ctgaatatct gcagacctcc cagaaaaggg taaatgtgga ggtgactgta
     2821 atcgggcggg cagtatcatc ccaaaacggg aatggcgagg ctgtacggca ccagtcactc
     2881 accggtgaac aaaggccggg aagctgaaaa aggaggaatg cttatggcaa aacgactggg
     2941 agtcacaggc ggaataggca gcggcaagac aaccgtatgc aggattttca gggttcttgg
     3001 aatccctgtg tttgttgctg acaatgttgc gaaggatgtg atggataatg agcctgacat
     3061 ccgtgcagca ataaacctga taaccggcaa ggatctctat gcccatggaa aactcaaccg
     3121 caaggagctt gcacggataa tcttcaacaa gcctgatctt ctacgtaaag tgaatgccgc
     3181 agtgcatccg gaggtgcttg aacgtttcaa acggtgggcc tccgcttcag cagctcccta
     3241 tgttattatg gaggctgcca ttctctttga ggcaggagct gattcactgg tagacagggt
     3301 ggttacagtg tccgcacctg tggaagagcg tat
//
LOCUS       DAOPBK010000231         3323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_22935_length_3323_cov_4.690636, whole genome shotgun sequence.
ACCESSION   DAOPBK010000231 DAOPBK010000000
VERSION     DAOPBK010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22935_length_3323_cov_4.690636"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1810)
                     /gene="ftcD"
                     /locus_tag="PLA17_10500"
     CDS             complement(98..1810)
                     /gene="ftcD"
                     /locus_tag="PLA17_10500"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPK85883.1"
                     /translation="MIANRLIECVPNFSEGRDMEKIKQITDEIESVEGVMLLDVDPGS
                     DTNRTVVTFVGEPEAVCEAAFRAVRKAARVIDMSKHSGAHPRMGATDVCPLVPVSGIT
                     MEETVEFARHLARRIGEELSLPVYCYEAAAFEEKRRNLANVRSGEYEGLKKKLEDPAW
                     RPDFGPARFDAGAGSVIVGARDFLVAFNINLNTTSVRRANAIAFDVRERGREKRTGNP
                     VTGKVERDADGRPIMIPGTLKAVKAIGWYIPEYGFAQISMNLTNIGITPVHVAFDEVC
                     NKADARGVRVTGSELVGLIPLKAMLDAGRYFLHRQQRSAGVSDEELIRTAVRSMGLSE
                     LKPFRPEEKIIEYILRDRSRKKLVDLSLSSFTWETSSESPAPGGGSVSAAMGALGAAL
                     GTMVANLSSHKRGWDDRWNEFASWAEKGVEMQKRLLALVDEDTEAYNGIVKAFGMPKK
                     SEEEKAARDAAVEAATLHASVVPLTVMKEAFKVFELLREMIVNGNPNSVTDGAVGVLA
                     VRACIRGAFLNVRINVKELKDRAKAAELVSEASEIDRAAASMEEEVIPRVIKELAIEG
                     EILE"
     gene            complement(1900..3177)
                     /gene="hutI"
                     /locus_tag="PLA17_10505"
     CDS             complement(1900..3177)
                     /gene="hutI"
                     /locus_tag="PLA17_10505"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPK85884.1"
                     /translation="MSLIIHNTGMLVQTREDGALFTAGKEMAVLPVIENGWLLIEDGI
                     IAGYGPAETMPGFSSAVAKLDAGGGYLFPAFCDPHTHLVYAGSREKEYTDKIRGLSYE
                     EIAKRGGGILNSAKLLHETSEDELFRQSMERVNEIISFGTGAVEIKSGYGLTTEDELK
                     MLRVIRRIKESSPLTVRSTFLGAHAVPAEYRHDRDGYVDLIVNEMIPAVAAERLADYI
                     DVFCDRGFFTVEETARILEAGVRHGMRPKIHANELDYSGGIQTGVRYGALSVDHLEFT
                     GDDEIAALLGSGTMPTLLPGAAFFLGMIDPPARKMINAGLPLAMASDYNPGSSPSGNM
                     KLVVSLGCIRLRLLPEEAINAVTINAAYAMGLAETHGSITVGKKANLFITKKIPSYEF
                     LPYAYGSNLVDTVLLNGEVVFSRQSAGGSPQQC"
BASE COUNT          759 a          994 c          736 g          834 t
ORIGIN      
        1 gaagcagatg caatgatctc atcttttatc cttgacaggc agcagactct ttcaggaaag
       61 cctgcccaaa actcaatgtt aatttagatt cccggagtta ttccaagatt tccccttcaa
      121 tggcaagctc ttttattacc cggggtatta cctcttcttc cattgaggct gcagccctat
      181 ctatttcact ggcttcagaa accagctccg ccgctttagc cctgtccttt aattccttga
      241 cattgatcct gacattcagg aaggcgcctc ttatacatgc cctgacggca agcaccccca
      301 cagctccgtc agtgactgag ttcggattgc cgtttacgat catctcccgt aacagttcaa
      361 agaccttgaa tgcttccttc atcactgtca ggggaacaac agaggcatgg agcgttgctg
      421 cctctaccgc cgcatccctg gcagctttct cctcctcact cttcttcggc atcccgaagg
      481 ccttcacaat cccgttataa gcttcagtat cctcatccac cagggcaagc agccgcttct
      541 gcatctcaac ccccttttca gcccaggagg caaactcatt ccagcggtca tcccaccctc
      601 ttttgtgtga ggagaggttg gcaaccatcg ttcccagcgc agcgccaagg gcacccatgg
      661 ctgcagagac agagccgcct cccggtgcag gtgattcgga ggaggtctcc catgtaaatg
      721 atgacagtga caggtcaacc agcttctttc tgctcctgtc cctcagtata tactctatga
      781 tcttctcttc aggcctgaaa ggcttcagct cagaaagccc cattgacctg acagcagttc
      841 tgatcagctc ttcatctgac actccggcag atctctgctg gcgatgcagg aagtaacgcc
      901 ctgcatcaag catggccttc agcgggatta gtccaaccag ctctgacccg gtgaccctca
      961 cgccacgggc gtcagccttg ttgcaaacct catcaaaggc aacatgtacc ggggtaatgc
     1021 cgatattggt caggttcata gatatctgcg caaaaccata ctccggaata taccatccaa
     1081 tggccttaac agccttcagt gtgccgggta tcatgatcgg tctgccatca gcatcccttt
     1141 caacctttcc tgtaacagga ttgcctgtgc gcttttcccg gcctcgttcc ctgacgtcga
     1201 atgctatggc attagcacgg cggacggagg ttgtgttcag gtttatatta aaggctacca
     1261 gaaaatcacg ggcgcccaca atcacagacc cggcgccggc atcaaaccgt gcaggcccga
     1321 aatcaggcct ccatgccgga tcttcaagct tcttcttcaa tccctcatac tcaccggacc
     1381 ttacgtttgc aaggttcctc ctcttctcct caaaggccgc ggcctcatag cagtacacag
     1441 gaagcgacaa ttcctccccg attctgcgcg caagatggcg tgcaaattct accgtctcct
     1501 ccatggtaat tccggagacg ggaacgagcg ggcacacatc ggtagctccc atgcgcgggt
     1561 gggcaccgct gtgctttgac atgtctatta ccctcgccgc cttcctgact gccctgaatg
     1621 cagcctcaca aaccgcctca ggctcaccta caaaagtaac aaccgtacgg ttggtatcac
     1681 tgcccggatc aacgtcaagc agcatgacac cctcaactga ttcgatctca tcagtgatct
     1741 gtttgatttt ttccatgtcg cgtccctcgc tgaaattggg cacgcattct ataagacggt
     1801 tcgctatcat aatattggat ttttaattct cgatcccctc cggggaatat attaattgtg
     1861 ttgcttgtgc aattctataa atactgccgg ctgttgctgt taacactgtt gcgggctgcc
     1921 gcctgcggat tgccggctga agacaacttc tccattcagc aaaacagtat ccaccaggtt
     1981 actgccatat gcataaggca gaaactcata agacggtatc ttcttcgtaa tgaagagatt
     2041 tgccttcttg cctacggtga ttgatccgtg cgtctccgca aggcccatgg catatgccgc
     2101 gttgatagtc accgcattta ttgcctcttc cgggagcagg cgcagccgta tgcatcccag
     2161 ggagaccacc agcttcatat tgcctgaagg cgatgagcca gggttgtaat cacttgccat
     2221 cgcaagtggc agtcccgcat taatcatctt ccgtgcagga ggatcaatca tcccgagaaa
     2281 gaaggctgcg ccgggcagca gcgttggcat ggtaccgctt cccagcaatg ctgcaatctc
     2341 atcatcacct gtaaactcca ggtgatcgac ggaaagtgcg ccatacctga ccccggtctg
     2401 tattccccct gagtaatcaa gctcgtttgc atgtatcttg gggcgcatac cgtgccgcac
     2461 gccggcctcg agtatacggg cagtctcttc cacggtgaag aaaccccggt cacagaacac
     2521 atctatgtaa tcagccagcc gttcggcagc aacagccggt atcatctcat tcacaatcag
     2581 gtcaacatat ccgtccctgt catggcggta ctcggccggc acggcatgcg ccccgagaaa
     2641 agttgacctg acggtcaggg gcgatgactc ctttatcctc cttataaccc tgagcatctt
     2701 gagctcatct tccgtggtca ggccatagcc gctctttatc tctacagctc cggtgccaaa
     2761 ggatatgatc tcgttcaccc tctccatcga ctgccggaag agctcatcct ctgaagtctc
     2821 atgcaacagt ttcgccgaat taaggatgcc acctcccctt ttggcaatct cctcgtatga
     2881 caatccgcgt atcttgtcag tatattcctt ctccctgctt cccgcgtaaa ccaggtgggt
     2941 atgaggatcg cagaaggccg gaaacagata acctcccccg gcatcaagct tcgcgacagc
     3001 gctgctgaaa ccgggcattg tctctgccgg accgtagccg gctatgatac cgtcttctat
     3061 cagcaaccag ccgttctcaa ttacgggcag taccgccatc tcctttccgg ccgtgaagag
     3121 cgcaccatct tccctggtct gcaccagcat ccccgtgttg tgaattatca ggctcatggc
     3181 actgttatta atgtccgcta atgtagcaat attaaaataa ttaggatatg agcatcattt
     3241 ttaattaact ttggtatccc tataaagatg aaaaccgcat gtatcaaaca aacaaacatg
     3301 ttgatatgat tctcagtcat gtg
//
LOCUS       DAOPBK010000232         3286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_23240_length_3286_cov_4.835655, whole genome shotgun sequence.
ACCESSION   DAOPBK010000232 DAOPBK010000000
VERSION     DAOPBK010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23240_length_3286_cov_4.835655"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..79)
                     /locus_tag="PLA17_10510"
     CDS             complement(<1..79)
                     /locus_tag="PLA17_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPK85885.1"
                     /translation="MERNLRKERTGVVVSNKMDKSIVVAI"
     gene            complement(90..293)
                     /gene="rpmC"
                     /locus_tag="PLA17_10515"
     CDS             complement(90..293)
                     /gene="rpmC"
                     /locus_tag="PLA17_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPK85886.1"
                     /translation="MKSSEIRELSNQELLERIDNEKTALVRMRLNHAISPLDNPQKIK
                     ESRKTIARLMTEMRKREISKKTK"
     gene            complement(306..728)
                     /gene="rplP"
                     /locus_tag="PLA17_10520"
     CDS             complement(306..728)
                     /gene="rplP"
                     /locus_tag="PLA17_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPK85887.1"
                     /translation="MLQPKKTKYRRSQKGKMKGNAQRGNQLAFGSFGIKTLEEAWITG
                     RQLEAARQAVTRYMKREGQIWIRVFPDKPITKKPAEVRMGKGKGAPEGFVVPVTPGRI
                     IIEAEGVPFEVAREALRLAAGKLPVATKFVVRHDYAVE"
     gene            complement(763..1497)
                     /gene="rpsC"
                     /locus_tag="PLA17_10525"
     CDS             complement(763..1497)
                     /gene="rpsC"
                     /locus_tag="PLA17_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPK85888.1"
                     /translation="MGQKVNPIGNRIGIIKGWDSNWYGGNDFSGKLVEDTKIREYLNA
                     RLAKASLSKIVIERTLKLITVTVNTARPGIIIGKGGQEVDKLKEELKKITRKEVQINI
                     FEVKRPELDAAIVASNIARQVEGKISYRRAIKMAIASTMRMGAEGIKVVISGRLGGAE
                     MARKETYKDGRIPLHTFRADIDYALGEALTKVGLIGIKVWICNGEVYGKRDLSPNIGA
                     KNIKGGKAPTGGYRKRSDRGDRGSKR"
     gene            complement(1501..1920)
                     /gene="rplV"
                     /locus_tag="PLA17_10530"
     CDS             complement(1501..1920)
                     /gene="rplV"
                     /locus_tag="PLA17_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPK85889.1"
                     /translation="MGARKRISAAARKEAAKEKAQARLRNVPTSPRKMRLVTDLIRGQ
                     EVNRALDILKFSKQEASRRLEKLLLSAIANWQAKNEGVRLEESDLYVKEVQVDGGRIL
                     RRIRPAPQGRAYRIRKRSNHVTLVLGSRKQEVENISE"
     gene            complement(1955..2221)
                     /gene="rpsS"
                     /locus_tag="PLA17_10535"
     CDS             complement(1955..2221)
                     /gene="rpsS"
                     /locus_tag="PLA17_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPK85890.1"
                     /translation="MSRSIKKGPFIDIHLSKKVEAMNDSGKKTVIKTWSRRSVISPDF
                     VGHTLAVHNGNKFIPVYITENMVGHKLGEFAPTRIFRGHGGKQR"
     gene            complement(2230..3054)
                     /gene="rplB"
                     /locus_tag="PLA17_10540"
     CDS             complement(2230..3054)
                     /gene="rplB"
                     /locus_tag="PLA17_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPK85891.1"
                     /translation="MAVRKIKPVTPGQRHKITSNFEDVTRDKPEKSLLAPLKKSGGRN
                     VDGRRTMRYLGGGHKRMYRVIDFQRTKDGVAAKVTSIEYDPNRSARIALLTYEDGEKR
                     YIIAPNGLKVGQEVMSGKTAHPEVGNTLFLEDIPLGTLVHNIELKPGRGAALARSAGT
                     FAQLAARDGKHAIIKLPSGETRMVLTACRATIGIVSNTDHNQERSGKAGRKRWLGRRP
                     RTRGVAMNPVDHPMGGGEGRASGGHPRSRSGMPAKGFKTRSRTKYSDRHIIEKRKK"
     gene            complement(3060..>3286)
                     /gene="rplW"
                     /locus_tag="PLA17_10545"
     CDS             complement(3060..>3286)
                     /gene="rplW"
                     /locus_tag="PLA17_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPK85892.1"
                     /translation="YGFLVNRKANKLQIKKAVEQLYGVNVESVNTINYGGKKKTRYTR
                     SGVTTGRTAATKKAVVTLAKGETIDFYSNI"
BASE COUNT          627 a          910 c          745 g         1004 t
ORIGIN      
        1 gaatggcaac gacgatagat ttgtccatct tattgctgac gacaacacct gtacgttctt
       61 ttctaaggtt cctttccatg ctgatacggc tattttgttt tcttactgat ctctctcttg
      121 cgcatttctg tcatgagcct ggcaattgtt ttcctgctct ccttgatctt ctggggatta
      181 tcaagtggag agattgcgtg gtttagtctc atgcgcacaa gtgcggtctt ctcgttgtca
      241 atacgttcaa gaagctcctg gttgcttaat tctcttatct ctgaactttt cattgcttct
      301 tctttttatt ccactgcgta atcatgacgc accacgaatt ttgttgccac cggcagttta
      361 ccggctgcga ggcgcagtgc ttcccttgcc acttcaaagg gaacgccttc ggcttcaatg
      421 atgatgcggc cgggggtaac gggaaccaca aatccttcgg gcgcaccctt acctttaccc
      481 atacgcacct ctgcaggctt cttggttatc ggtttatcag ggaagacacg gatccatatc
      541 tggccttcac gtttcatgta acgggtcaca gcctggcgtg cagcctcaag ctgacggccg
      601 gtaatccatg cctcctcaag ggttttgatg ccaaatgatc cgaatgccag ctgattgcct
      661 ctctgggcat tgcctttcat cttgcccttc tggctcctcc tgtacttggt cttcttcggt
      721 tgtaacatcg ctatattata tttgttgttt tacttatctc cattacctct tgcttccacg
      781 gtcaccgcgg tcgctccttt tcctgtagcc gcctgtgggt gccttgcctc ccttgatgtt
      841 cttcgcacca atgttggggc tgaggtcacg cttgccatac acttcaccat tgcatatcca
      901 taccttgatg ccgatgaggc caaccttggt gagggcttcg ccaagggcgt agtcaatgtc
      961 tgcacggaaa gtatggagag gaatcctgcc atccttgtag gtctccttgc gggccatttc
     1021 tgcaccgccg agcctgcctg agataacaac ctttatacct tcggcgccca tgcgcatcgt
     1081 cgaggcaatg gccatcttta tggcgcggcg atatgagatc tttccctcta cctggcgtgc
     1141 tatgttgctg gcaactattg cagcatcaag ctccggacgc tttacctcga agatgtttat
     1201 ctgaacctcc ttcctggtga tcttcttgag ctcttccttc agcttgtcaa cctcctgccc
     1261 gcctttgcca atgatgatgc ccgggcgtgc cgtgtttacg gttacggtta tgagcttgag
     1321 ggtacgttca atgacgatct tcgaaagact tgccttggca agcctggcat tcaggtattc
     1381 cctgattttg gtgtcttcaa caagtttgcc tgagaagtca ttgccaccat accagtttga
     1441 gtcccatccc ttgatgattc ctattctgtt gcctatcgga tttactttct gtcccattgt
     1501 ttactctgaa atattttcaa cttcctgttt cctgctaccc agcacaaggg ttacatggtt
     1561 tgatctcttg cgtatacggt aagcccttcc ctggggggca ggccgtatac ggcgcagaat
     1621 gcggccgccg tcaacctgaa cctccttcac gtaaaggtca ctctcttcaa gacggactcc
     1681 ttcattcttt gcctgccagt tggcgatggc cgagaggaga agtttttcca gtctgcgcga
     1741 agcttcctgc ttgctgaact tcaggatatc aagagccctg ttcacttcct ggccacgtat
     1801 aagatcggta accagcctca ttttgcgcgg tgaggtcggc acattgcgaa gacgtgcctg
     1861 agccttctcc ttagcggctt ccttccttgc tgctgcgctt attctttttc ttgcacccat
     1921 ttctgattct ttttcagttt ctatgccaca ttacctatct ctgttttcca ccatgaccgc
     1981 ggaaaatacg ggtaggggca aactcgccca gcttatgacc aaccatgttc tcggtaatgt
     2041 aaacaggtat gaatttgttt ccgttatgca ctgccagggt gtggcccaca aaatcgggtg
     2101 aaataactga tcttctcgac caggtcttga tcacagtttt ctttccgctg tcattcattg
     2161 cctccacctt tttgctcagg tgaatatcga tgaatgggcc ttttttaata gatctgctca
     2221 tcgtatcaat tattttttcc ttttttcaat aatatgcctg tcagaatact tggtacggct
     2281 ccttgtcttg aaacccttgg caggcattcc gctgcgtgag cgcgggtgac caccggaggc
     2341 tcttccttca ccgccaccca ttggatggtc aaccgggttc attgcaacac ccctggtcct
     2401 tggcctgcgg ccgagccagc gcttgcggcc tgcctttcct gacctctcct ggttgtggtc
     2461 agtgttcgaa acaattccta tcgtggcgcg gcatgcagta agcaccatcc tggtctctcc
     2521 cgaggggagc ttgatgattg catgcttgcc gtcacgggca gccagctgtg caaaagtacc
     2581 tgcactgcgg gccaatgctg cacccctgcc aggcttcagc tctatgttgt gaaccagggt
     2641 gccgagagga atgtcttcaa gaaaaagagt gtttccaacc tcagggtgtg ccgtctttcc
     2701 tgacatcacc tcctggccaa ccttcagacc gttgggagca attatatacc tcttctctcc
     2761 atcttcatag gtaaggaggg caatacgtgc cgaacggttc ggatcatatt cgatagaagt
     2821 taccttagcg gcaacaccgt ccttggtcct ctggaaatcg atcacacggt acattctctt
     2881 gtgaccgcca ccgaggtacc tcatggtccg cctgccgtca acgttccttc ctcctgactt
     2941 cttcaacggt gcgagcaagg atttctccgg cttgtcacgg gttacatctt caaagttact
     3001 cgtgatctta tgtctctggc ctggtgttac aggcttgatc tttctgactg ccatattaat
     3061 taaatgttgc tgtaaaagtc tatagtctcc cctttggcga gagtgacaac agcctttttc
     3121 gttgctgccg tccggcctgt tgtcacacct gacctggtat agcgggtctt tttctttcca
     3181 ccatagttga tggtgttgac actctctaca ttgaccccgt aaagctgctc aacagccttc
     3241 ttgatctgaa gcttgttggc tttacggtta accaggaaac catacc
//
LOCUS       DAOPBK010000233         3286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_23241_length_3286_cov_4.494893, whole genome shotgun sequence.
ACCESSION   DAOPBK010000233 DAOPBK010000000
VERSION     DAOPBK010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23241_length_3286_cov_4.494893"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLA17_10550"
     CDS             <1..206
                     /locus_tag="PLA17_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPK85893.1"
                     /translation="GSFTVTGMPDHVSRIEISDLRGNLMMSMKTGRGKHEITLDTDIV
                     SGIYLVTLYGDNYTATRKLIIKK"
     gene            219..3071
                     /locus_tag="PLA17_10555"
     CDS             219..3071
                     /locus_tag="PLA17_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPK85894.1"
                     /translation="MRRSLSALIPVKALIAALAFQSCSMTPYERTDNGVIIRLDDDSF
                     RPGQAVRLIVVNERIIRVSSVPFGEFPETTSLMAVERPDANMEFTIEKGAGHVTLSTS
                     LLSAEVSLLTGAVSFTDNDGTLFLTEKEDGGRSFMPVMAMGEKGYTIRQQFESYPDEA
                     IYGLGANQTSFMNLKGKDADLFQYNTLAVVPFIISNRNYGILWDNNSRTKYGDIRDWN
                     ELSSLKLYDSQGAENGLTATYADRKTGKQVFTTRNEREISYQFIPDLAGFPEGFNPGE
                     GKVTWEGAFETDTTGVFKFMFNSAGYARLWIDSRLLFDRWRQCWNASASYFTLNLEQG
                     RRYPLKIEWIPDGGESYIALRYLTPLDDDEQQRISFWSEVADQIDYYFISGENMDEVI
                     SGYRTVTGKAPVMPEWAMGFWQSRQRYTNQEELLDVVREYRKRNIPFDNIVLDWQYWP
                     ADKWGDHQFDVTRFPDPDGMIRTLHDDLNARIMISVWPKYYVGTENYEAMKSKGYLYM
                     RNVEMERRDWIGYLSTFYDAFNADARTAFWNQINIALYSRGIDAWWLDATEPDITSNL
                     PMEERKAMMNPTALGSADRYFNAYSLVQAQGVYEGQRATDPENRVFILTRSAFAGLQR
                     YSAANWSGDIAARWHDMAAQIPCGLNMSMSGIPWWTMDIGGFSVESRYHNPSPADLDE
                     WRELMTRWHQYGAFVPLFRSHGEFPFREIYNTAPDNHIAYKTMVEYNRLRYRLMPYIY
                     SLAGQAWLNDYTIMRGLTMDFSLDTEVFDIADQYMFGPSLMVNPVTEYKARSRNVYLP
                     SAYGWYDVRTGRHYAGGTVVEADAPYEWMPLFAREGSLIPTGPDIQYTGEKEADPLTL
                     WVYAGADGAFELYEDEGDNYNYEDGAYTLIPLTWNEAERTLTIGKREGEYRGMLHERT
                     INVIMVSENRAVKLDFGRPPDKSVKYSGEEIKVRFE"
BASE COUNT          850 a          781 c          891 g          764 t
ORIGIN      
        1 aaggcagctt caccgtcacc ggcatgcctg atcatgtcag cagaattgag atcagcgatc
       61 ttcggggaaa ccttatgatg agcatgaaga cagggcgtgg aaaacatgag attacccttg
      121 atactgacat tgtttccggc atttatcttg taactttata cggagataac tacactgcaa
      181 caaggaaatt gataatcaaa aagtaacccg atatgaaaat gagacgatca ctgtctgcac
      241 tgattccggt aaaggcactc attgcggctc tggcgttcca gtcatgctcc atgacaccat
      301 acgaacggac tgataacggt gtgatcatac ggctggacga tgactctttc cgtcccggtc
      361 aggctgtgag gcttattgtt gtaaatgaaa ggataatacg ggtttcttca gttcctttcg
      421 gtgagtttcc ggaaacaacc agcctcatgg ctgtggagcg gcctgatgcc aatatggagt
      481 ttaccatcga gaagggtgca gggcatgtaa ccctcagcac ttcactgctc agcgccgagg
      541 tgtcactact gacgggagca gtctctttta ccgataatga cggaactctc ttcctcactg
      601 aaaaggaaga cggtggaagg agtttcatgc ctgtcatggc aatgggtgaa aaagggtaca
      661 ccatcagaca gcagtttgag tcttatcccg atgaagccat ctacggactg ggagccaacc
      721 agacctcttt catgaacctg aaggggaaag atgctgatct ctttcaatac aacacactgg
      781 cagtggtgcc attcattatc tctaaccgca actacggaat cctctgggac aataactcaa
      841 gaacaaaata tggtgatatc cgcgactgga acgaactctc ctccctgaaa ctatatgaca
      901 gtcagggtgc tgagaacggt ctgacagcga cgtatgctga ccggaagaca gggaaacagg
      961 tatttaccac ccggaatgaa agggagataa gctaccagtt catacctgac cttgccggat
     1021 ttcctgaagg gtttaacccc ggagagggaa aggtaacctg ggagggggcg tttgagaccg
     1081 acacaacagg ggtattcaag ttcatgttca actcggcagg ttatgccagg ctctggattg
     1141 acagcaggct gctttttgac cgctggcgtc agtgctggaa cgcttcagcc agctatttca
     1201 ccctgaacct tgaacagggc cggcgttatc ccctgaagat agagtggatt cctgacgggg
     1261 gcgagtcata catagccctc aggtatctca ctcccctgga tgatgatgaa cagcagcgca
     1321 tttcgttctg gtcagaggtg gctgaccaga tcgactacta tttcatcagt ggcgaaaaca
     1381 tggatgaggt tatcagtggt tatcgcaccg ttaccgggaa agcccctgtg atgcctgaat
     1441 gggctatggg attctggcag agccgtcagc gctacacaaa ccaggaggag ttgcttgatg
     1501 ttgtcaggga gtacaggaag agaaacatac cttttgacaa tatagtactc gactggcaat
     1561 actggccggc agacaaatgg ggtgatcacc agtttgatgt aacccggttc cctgatcctg
     1621 atggcatgat ccggacgctg catgatgacc tgaacgcaag aatcatgatc tccgtctggc
     1681 cgaaatacta cgtcggcaca gaaaactatg aggccatgaa gagcaagggt tatctctata
     1741 tgcgtaacgt tgagatggag cgcagggact ggatcgggta tctctccacc ttttatgatg
     1801 cattcaatgc cgatgcacgg actgcattct ggaatcagat caacattgca ctatacagca
     1861 ggggcataga tgcctggtgg cttgacgcca cagagccgga tatcacatca aacctcccta
     1921 tggaggagcg caaggctatg atgaatccga cagcgcttgg ctcggctgac aggtacttca
     1981 atgcctattc gcttgtgcag gctcagggtg tctatgaggg acagcgggcc actgacccgg
     2041 agaacagggt tttcatcctc acccgttcgg ccttcgcagg cctgcagcgc tactccgcag
     2101 ccaactggag cggtgacata gctgcaaggt ggcatgacat ggcagcgcag ataccctgcg
     2161 gactgaacat gtcgatgtca ggcatcccct ggtggaccat ggacatcgga ggcttctccg
     2221 tggaaagccg ttatcataat ccatccccgg ccgaccttga cgaatggcgc gagctgatga
     2281 cccggtggca ccagtacggg gcgtttgttc cgctcttccg ctcacacggc gagttcccat
     2341 tcagggagat ctataacacc gctcccgata accatatagc ctataagaca atggttgagt
     2401 ataaccggct gagatacagg cttatgccct atatttactc gctcgccgga caggcatggc
     2461 tcaatgatta cactatcatg agagggctga cgatggattt cagccttgac actgaggtgt
     2521 ttgacatcgc cgatcagtat atgttcggcc cttcgcttat ggttaatcct gttacggaat
     2581 acaaggcacg gtcgcgtaat gtatatctgc cctcggcgta tggctggtat gacgtgcgca
     2641 ccggaaggca ttacgccgga gggacagttg ttgaggcaga tgctccctat gagtggatgc
     2701 ctctgttcgc cagggaaggc tcactcatcc cgacaggacc agacatccag tataccgggg
     2761 agaaggaggc tgatcccctt acattatggg tctatgccgg cgctgacggt gcctttgagc
     2821 tgtatgaaga tgagggcgac aactataatt atgaggatgg agcatatacc ctcatacccc
     2881 ttacctggaa cgaggctgaa cgtaccctga ccatcgggaa aagggaaggg gaataccggg
     2941 gaatgctcca tgagcgaacc ataaatgtca taatggtatc tgagaatagg gccgtgaagc
     3001 ttgacttcgg gcggcccccc gacaaatcgg ttaagtattc aggggaggaa ataaaggtca
     3061 ggtttgagta attcctgagg ttgaggtata acttgcagcg ttctgctcag atcactcttt
     3121 ttggtaagat tcgggtaatt cctgaagtca gggcataaca gaaacaatat atataaatga
     3181 ttgtgggaat gaagaaagtt atcactgtca taatggcaat gctgttgttg gcagcaactg
     3241 ccggggcaca gagtatagtg cctgctgagt ggaaattcct tacggg
//
LOCUS       DAOPBK010000234         3260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_23495_length_3260_cov_5.250858, whole genome shotgun sequence.
ACCESSION   DAOPBK010000234 DAOPBK010000000
VERSION     DAOPBK010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23495_length_3260_cov_5.250858"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3238)
                     /locus_tag="PLA17_10560"
     CDS             complement(<1..3238)
                     /locus_tag="PLA17_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPK85895.1"
                     /translation="MDGFAQGTKYEMIFIPRLLAFILLLGACRMDGFAQGTKYEAENG
                     ILTGSVTVQTTVAGFSGTGYTGLFENEGDMVAVTFNLSPAAGYSLYIGYAAPYGDKKN
                     ILTINGNSIEASFPASAGFTEIALGKVWLREGSNTISITKSWGWFLLDYFRIEPCTEP
                     EVTVQLPYKLSTRAPHLETRMLWSYLMDSFTQRIHSGVMNLNAREEAEWLFALTGRYP
                     ALTGLDFMNHTRNYSWYDKSVVVNEAMNWYNQNGLVAICWHWRDPSRATEEFYTSGTA
                     FDVSKITETTSAEYQMMLSDIDIIAGYLKQLNASKVPVLFRPLHEASGRWFWWGAKGP
                     EPCKALWRIMFDRLVNYHGLKNLIWVWTTDAAPDNLDWYPGDEYVDILGADIYAADGD
                     FSSQLLTYNAIKEKFGGRKLITLSENGPVPDPDRLVSDRAHWSWFMPWYGSFIRDGIK
                     NPPAHWQKVMSHDYVVTLDEMPDLKSYPLSDEPDYSAFPQGFFMAGWKPRTAVMPDYT
                     DVPAVTDPVTVAITVDCSDTVTLVSPYLFGDNANLWTGPMSDNTTLMKNITNRDQGVM
                     RGPGGSTSDAFFWNRSTRPPDVPQTLLNDPSNTNWPWYGQRAENWTMHVDSFYSILSK
                     AGITGMLTVNYGYARYGTGDDPVAQAAHLAADWVRYDNGRTKFWEIGNEVFGNWEAGY
                     RIDRSLNRDGQPEYITPQLYGQHCRVFIDSMRVAAAETGHDIKIGVVMVESATTHNSW
                     NAGVAAQVGDKADFYVVHSYYTPWNTDSDVATVLNSYKNTEGYINHVRSTVAAAGMPE
                     LPVAMTEYNIFAVGSRQQVSHANGMQAVLATGEMIRTGYGAACRWDLANGWDNGNDHG
                     MYSYNEPADPLDPLSPRIPDYTPHPAFFHLYYMRRHTGDVLLGSTVTGAPGVVITPTA
                     FSSGHLGASLVNTTKVQRVVRLNLKDYGVGNRFCTYTLTGTEGHDFSRKVFVNSTGGA
                     LAAGGPDSYETIRADAVVIGDEIRINLPPLSAVYILVEPGTRQLAISNEVTSADPVRS
                     DDSVTIWPNPSEGNFTVTGMPDHVSRMEISDLRGNLLLSRETGRGEHEV"
BASE COUNT          716 a          897 c          863 g          784 t
ORIGIN      
        1 taacctcatg ttctccacga cctgtttccc tgctcagcag aaggtttccg cgaagatcgc
       61 tgatctccat tctgctgaca tgatcaggca taccggtgac ggtgaagtta ccttcggagg
      121 gattaggcca gattgtgaca gaatcgtcag accttaccgg gtcagcggaa gtcacctcgt
      181 tactgatggc cagctgcctt gtgccgggct caaccagaat atagacggcc gagaggggag
      241 gaagatttat ccttatctca tcgcctatta caaccgcatc cgccctgatt gtttcatagc
      301 tgtcaggccc tccggccgcc agtgcgccgc cagtgctgtt gacaaacacc ttccttgaga
      361 aatcgtgccc ctccgtgcct gtaagagtat aggtgcagaa acgattgcca acgccgtaat
      421 ccttcaggtt aaggcgaacc accctttgca cctttgttgt attgacaagt gaagcgccca
      481 aatgaccgga gctgaatgcc gtaggtgtga tgaccactcc gggggcgccg gtgactgttg
      541 accccagcag tacatcaccg gtatgccggc gcatgtaata gaggtggaag aatgccgggt
      601 gaggagtgta gtccgggatt ctgggcgaga ggggatcaag gggatcggca ggttcgttat
      661 acgagtacat accgtggtca ttgccgttat cccagccgtt tgcaagatcc cagcggcagg
      721 ctgcaccata acctgtcctg atcatctcgc cggtggcaag cacagcctgc atcccgttgg
      781 cgtgcgagac ctgctgcctt gagcccactg caaagatgtt gtattcagtc atcgctaccg
      841 gcagctcagg catacctgct gccgccaccg tgctcctgac atggtttatg taaccttccg
      901 tgtttttgta ggaattaagc accgttgcca cgtcagagtc cgtgttccac ggggtgtagt
      961 aactatgcac aacatagaag tcggctttgt ctcccacctg tgcagccaca ccggcattcc
     1021 acgaattgtg ggtggtggca gattccacca tcactacgcc gatttttata tcatgaccag
     1081 tctctgcggc cgcaaccctc atggagtcaa tgaaaaccct gcagtgctgt ccgtaaagct
     1141 gaggggttat gtattcaggc tgcccgtccc tgttgagtga acggtcaatc cggtaaccgg
     1201 cttcccagtt gccaaacact tcgttcccta tctcccagaa cttcgtcctt ccattgtcat
     1261 atcgcaccca gtcagcagcc agatgggcgg cctgtgctac cggatcgtcg cctgtcccgt
     1321 accttgcgta gccatagttg acggtgagca ttccggtaat gcctgccttg ctcagtatgc
     1381 tgtaaaagga atcgacatgc atggtccagt tctccgccct ctgaccgtac cagggccagt
     1441 tggtgtttga cggatcattg agaagtgtct gtggcacatc cggcggtctt gtactcctgt
     1501 tccagaagaa ggcatcggag gtgctgcctc ccggcccgcg cataactccc tggtcacgat
     1561 tggtgatgtt cttcatcagg gtggtattgt cagacatggg ccctgtccaa agattggcat
     1621 tgtccccgaa gagataaggt gacaccagcg tgacagtgtc agagcagtca acggtaatgg
     1681 ctacggtaac aggatcggta actgcaggca catcggtata gtcaggcatg acggctgtcc
     1741 gcggtttcca tccggccatg aagaaaccct ggggaaaggc gctgtaatca ggctcgtcac
     1801 tgagcggata gctcttcagg tcaggcatct cgtcaagagt gaccacataa tcatgtgaca
     1861 tgaccttctg ccagtgagcg ggcggattct ttatcccgtc acggatgaaa gagccgtacc
     1921 agggcatgaa ccaggaccag tgagccctgt cggataccag cctgtcagga tcagggacgg
     1981 gtccgttctc gctcagggtg atgagttttc tgccgccgaa cttttccttt attgcattat
     2041 atgtaagcaa ctgtgagctg aaatcgccgt cagcggcata gatgtcagcc ccgaggatat
     2101 ccacatattc atcaccgggg taccagtcaa ggttgtcagg cgcggcatcg gttgtccata
     2161 cccagataag gtttttcagg ccatgatagt taaccagacg gtcaaacatg atccgccaga
     2221 gcgccttgca cggttccggc cctttggcac cccaccagaa ccacctgccg gaagcttcgt
     2281 gaaggggcct gaagaggacc gggactttac tggcgttcaa ttgcttcagg tagccggcaa
     2341 tgatgtctat atcactcagc atcatctggt attcggctga tgtggtctcg gtgatctttg
     2401 agacgtcaaa cgctgtaccc gaagtataaa actcttcagt agcccttgag gggtcacgcc
     2461 agtgccagca gatagccacc agtccgttct ggttgtacca gttcatggcc tcattcacca
     2521 ctaccgattt atcataccag ctgtagttgc gggtatggtt catgaaatca agtcctgtca
     2581 gcgcagggta cctgcccgtc agggcaaaca gccattcagc ctcttccctg gcattgaggt
     2641 tcatgacccc tgagtgaatc ctttgcgtga agctgtccat caggtagctc cagagcatcc
     2701 gtgtctcaag gtgaggggcc cgggttgaga gcttatacgg cagttgtaca gtgacctccg
     2761 gctccgtaca gggttcaatg cggaaatagt ccagcaggaa ccagccccag ctctttgtta
     2821 tcgagattgt gttgcttccc tccctgagcc agacctttcc cagggcaatc tcagtgaaac
     2881 cggccgatgc cgggaaagag gcctctatac tgttgccgtt gattgtgagg atattctttt
     2941 tatcgccgta aggcgcggcg taacctatgt aaaggctgta accggctgcc ggtgacaggt
     3001 tgaatgtaac tgccaccatg tcaccctcat tttcgaaaag ccccgtataa ccggtgcccg
     3061 aaaaacctgc aaccgttgtc tgcacggtaa cagatccggt caggatgcca ttctcagctt
     3121 cgtatttcgt gccctgtgca aatccatcca tccggcatgc gccaagtagt aatatgaatg
     3181 ccagcagcct gggaataaat atcatttcgt atttcgtgcc ctgtgcaaat ccatccatcc
     3241 ggcatgcgcc aagtagtaat
//
LOCUS       DAOPBK010000235         3251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_23580_length_3251_cov_3.472778, whole genome shotgun sequence.
ACCESSION   DAOPBK010000235 DAOPBK010000000
VERSION     DAOPBK010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23580_length_3251_cov_3.472778"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="PLA17_10565"
     CDS             complement(<1..2071)
                     /locus_tag="PLA17_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPK85896.1"
                     /translation="MKRLLSVFFLLCGLLLTSAAQQKTVTGMVTSSEDNLGIPGVTVL
                     IKGTTIGVVTDVDGNYSIQASPGQILVFRFTGQKTQEVTVGAANTIDIVMQPDLLNLE
                     EVVVVAYGVQKREASTGSVGEVKSSEIQNVPETSIEKMLSGKVAGVQVTATSGQPGAN
                     TQIRIRGISSINAGSEPLYVIDGIPVMSGDQSFFTNTGNALASLNPNDVESMTILKDA
                     AASSIYGSRAANGVILITTKSGKAGAAKMNFRASYGVEQIANDNGYRPLTAEEYLTFQ
                     RTAVINAGGNPDDPSNSKYYFPNSLLDGPITDWMKELTRTGSIYSAELSAQGGSDRTQ
                     HFFSGLYDKHEGIVYGSDFTKFSIRSNVDHQLNKKLKLGVRFNGAYSVANDVPMQAMY
                     YVNPLFAMMMIKPFTPIRNDDGTYNLIIPENANANPRATAEYDPQWEKQYRLNTNGFL
                     EYQIIKGLTAKTANSIELTDGEGVRYWSPEANYGETLGYLQTSRTKYVLLTTSNTLNF
                     DKEFGSHNLSIVGGQEASKFDYNEYYIVSPNVDPNIPFPNTATSDDDDADYYETAYTI
                     MSFFGVARYNYDNKYYLQASLRTDGSSRFGSKNRWGTFWSVGASWNIHKEHFMDNVEF
                     INMLKLRGSYGISGNFNIGNYAQYGLYGAGQYNGNSVMVPTQPSNPDIGWENNTGYNA
                     GLDFALFD"
     gene            2229..>3251
                     /locus_tag="PLA17_10570"
     CDS             2229..>3251
                     /locus_tag="PLA17_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPK85897.1"
                     /translation="MSSFVHLHVHTQYSILDGAAAVKPLVSRARELGMTAIAITDHGN
                     MFGVKGFHDAATEESIKPIIGCEIYVARTSLEDKTSIEDRSGDHLILLAKNKTGYQNL
                     VRLVSEAWIRGFYYKPRIDKELLLKHREGLIASSACIAGEAAQEILAGNMREAEEAIL
                     SYREIFGDDYYLEVQRHETTDPTADVTVFPQQQRVIAAYRELAAKHNIKIIASNDVHF
                     IMKDDAEAHDRLICLNTGKDLDDPKRLRYTKQEYLKSAEEMLSIFADMPEAVSNTVEL
                     ADKVEYYDLNSEPIMPEFTIPAEFADKDEYLRHLTMKGAAERWETITPEIEKRLDFEL
                     SVIKNMK"
BASE COUNT          892 a          760 c          760 g          839 t
ORIGIN      
        1 ggtcaaataa tgcaaagtca agacctgcgt tataaccggt attgttttcc catccaatat
       61 caggatttga tggctgggtg ggaaccataa cagagtttcc gttgtactgc cctgcaccgt
      121 aaagaccata ctgggcatag ttgccaatgt tgaagttacc gctgatacca tagcttccgc
      181 gaagcttaag catgttgatg aactctacat tgtccatgaa gtgctcctta tgaatgttcc
      241 atgatgcacc cactgaccag aaggtacccc agcggttctt tgatccgaaa cgtgacgaac
      301 cgtctgtcct gaggcttgcc tgcagataat acttattgtc gtaattatag cgggcaacac
      361 caaagaatga cattatggtg tatgctgtct cataataatc cgcatcatcg tcatccgagg
      421 tggccgtgtt tgggaacgga atattcgggt ctacgttggg agatacgata tagtactcgt
      481 tatagtcaaa ttttgaagct tcctgaccgc ctacaattga gaggttatgc gagccaaact
      541 ccttgtcgaa gttaagggtg tttgaggttg taagcagtac atacttggtt cttgaggtct
      601 gaaggtaacc cagtgtctca ccatagttgg cctcagggct ccagtatctt acaccttcac
      661 cgtcagttaa ttcaatactg ttggctgtct ttgctgtaag accctttata atctgatact
      721 caaggaatcc gtttgtgttg agcctgtact gcttttccca ctgcgggtca tactcggcag
      781 tggcacgcgg gttggcattg gcattctccg gaataatcag gttatatgta ccgtcatcat
      841 tcctgatcgg agtgaatggc ttgatcatca tcatggcaaa cagcgggttc acgtagtaca
      901 ttgcctgcat gggaacgtcg tttgcaactg aatatgctcc gttgaaacgg acacccagct
      961 tcagtttctt gttcagctga tggtcaacgt ttgaacgtat ggaaaacttg gtgaagtcag
     1021 aaccgtatac aataccctcg tgcttgtcat acaaacctga gaagaagtgc tgggtcctgt
     1081 cgctgccacc ctgtgcactc agctctgcag aatagatgct gcctgtacgg gtgagctctt
     1141 tcatccagtc agtaataggt ccgtcaagaa gagaattcgg gaaatagtat ttcgagtttg
     1201 acggatcatc agggttacca cctgcattta tcacagccgt cctctggaag gtaaggtatt
     1261 cttctgccgt gagtggcctg taaccattgt cattggcaat ctgctcaaca ccatatgatg
     1321 cacggaaatt catctttgca gcaccggctt tacctgactt ggtagtaatg agaattacac
     1381 cgttggctgc ccttgaaccg tagatacttg aggcagcggc atccttgagg atcgtcattg
     1441 actcaacatc attcgggttg agtgaagcaa gtgcattacc tgtgtttgta aagaaactct
     1501 ggtcacctga catcacggga ataccatcaa taacgtacag gggttccgag cctgcattga
     1561 ttgagctgat accacggatt cgaatctggg tatttgcacc aggctgccct gatgttgcag
     1621 ttacctgaac accagccacc tttccggaaa gcattttctc aatacttgtc tcaggaacgt
     1681 tctgaatctc cgaactcttt acttcgccta ccgatcccgt gctggcttca cgcttctgaa
     1741 caccgtatgc cacaacaaca acctcttcga ggttaagaag gtcaggctgc attacaatat
     1801 caattgtatt ggcagcacct acagtgacct cctgcgtttt ctgaccggta aacctgaaaa
     1861 caagaatttg gcccggtgag gcctgaattg agtagtttcc gtctacgtcg gtaactactc
     1921 caattgtggt tcctttaatc aaaacggtta ctcctggtat cccgaggttg tcctcagagg
     1981 aagtaaccat tccggtaact gtcttctgct gggcagcaga agtcagcagt aatccacaaa
     2041 gcaagaaaaa tactgaaagt agtcttttca taactcttag attaggttaa tactaaacaa
     2101 aatgtagttt caaataaagc aggtaaatat aagaaaaaat atttgcatga aataagttgg
     2161 acataaacaa tctctatttt tccttagatt tgtaatctgg tgaggagaaa aacccgttta
     2221 gcacttttat gtcatcattt gttcatttac acgtacatac acaatactcc attcttgacg
     2281 gggcagcagc agtaaaacct ctggtcagca gggccaggga actgggaatg acggccattg
     2341 ccatcactga ccacggtaac atgttcggtg tgaaagggtt tcatgatgcc gctaccgaag
     2401 aatcaataaa gcccattata ggatgtgaga tctatgtggc ccggacaagc cttgaagaca
     2461 aaacatcgat agaggaccgc tccggtgacc acctcatatt actggcaaaa aacaaaaccg
     2521 gctaccagaa cctggtcagg ctggtctccg aagcctggat aagaggcttc tattacaagc
     2581 cccgtattga caaggagctt ctcctcaaac accgggaggg gctgatagcc tcctcagcct
     2641 gcatagccgg tgaggcagcc caggagatac tggcaggaaa catgcgggag gcagaggaag
     2701 ccatcctttc atacagggag attttcggcg acgattacta tcttgaagtg cagcgccatg
     2761 aaacaactga cccgacagca gacgtcaccg tctttccgca gcagcaaagg gtgatagctg
     2821 cataccgtga acttgctgca aaacataata ttaaaataat tgcttcgaat gacgtccact
     2881 tcataatgaa ggatgacgcc gaggcccatg accgtctcat ctgcctcaat acaggcaagg
     2941 acctcgatga cccgaaaagg cttcgatata ccaaacagga gtatctgaag agcgcagaag
     3001 agatgctcag catctttgcc gatatgccag aggcggtcag caacactgtg gaactggctg
     3061 acaaggttga atattatgat ctcaacagcg agcccataat gccggaattc accataccgg
     3121 cggaatttgc cgacaaggac gagtatctca ggcacctgac aatgaaaggt gcagcagagc
     3181 ggtgggagac tatcacaccc gaaatagaga aacgtcttga ctttgagctc tcggtaataa
     3241 aaaatatgaa g
//
LOCUS       DAOPBK010000236         3211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_23941_length_3211_cov_5.005070, whole genome shotgun sequence.
ACCESSION   DAOPBK010000236 DAOPBK010000000
VERSION     DAOPBK010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23941_length_3211_cov_5.005070"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(557..1744)
                     /locus_tag="PLA17_10575"
     CDS             complement(557..1744)
                     /locus_tag="PLA17_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85898.1"
                     /translation="MTKPGNIQSKAFTIGMLGVLLFSPSCKPGSGTGAEAVSPDNEFR
                     SVFYSDSGGISGADGLFSFPLPDGSSIFLLGDCFIGEVRSGARDTSTPMLRNAFNLVN
                     KEKTAAMAIVRGTIDAPLTLMEPVNEPGDSTYRWYWPGHGFIRNDTIYVFALSLVNDT
                     SAVINFGLPEGADDETVDMAEMIFSFRISHIDLLSFSYPDFRHLETHRTEIDYPVSQI
                     DFGNCVMVDNGYVYIFGTRNLSAKAEIHAARVPFNSSTLYRDWEFSTGAGWERDISRA
                     APLDIDISVSEQFSIFRHRNKYILLTQERAGADIYTYVSDFPDRGFHNKKFIYHTAET
                     EADTSKRVFTYNALAHMQYIENNRLLVSYCVNSLNPGDVFSDVEKYRARFIRVPIKLI
                     LRE"
     gene            complement(1789..2985)
                     /locus_tag="PLA17_10580"
     CDS             complement(1789..2985)
                     /locus_tag="PLA17_10580"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HPK85899.1"
                     /translation="MKSRILAVISVVVIVSGCNPKPGSDAARNQSESLQPMTFTSEPA
                     PEWTALMERTSGWFGADGIFSIPLDGIEDQQNKDKKTLFIFSDTYIGEVKDNVPQAGN
                     VMVNNTTAWMSGIIPERQAIVFEYKKNSDGSPATYFTPDNSNSTEGEYFWLGDGFINH
                     ARKNSLYIFAYHVHKTGPNVFDFEATNVALLKIKNPAPEGIKVYEQIPTGLGFIHPVL
                     GRTYFGSGIFVNTRKAGAPNPDGYVYIYGIAEGKKSLLAARTRPADFEETDRWAFWNG
                     EQWVEGKENIAPITDDISNELSVTPTGDGRYLLTFTVMGISDKIGIRVGNSPVGPFGE
                     INEVYTCPEYAEKGLLPYNAKAHYHLSKPGELLVSYNTITLNFWEDIRKDATIYHPRF
                     IKVRYK"
     gene            complement(3020..>3211)
                     /locus_tag="PLA17_10585"
     CDS             complement(3020..>3211)
                     /locus_tag="PLA17_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL family lipase"
                     /protein_id="HPK85900.1"
                     /translation="DIIRINELLKQIASENRLVYIDLFTLFRLENDKLNPAYSIDGLH
                     LNGKGYLVWKDAIIKYVKE"
BASE COUNT          840 a          758 c          700 g          913 t
ORIGIN      
        1 tatcgctgcg ctataaaaca aaaaccgatc ctgtaaaact tcgatgaaaa cctgccaccc
       61 cagacacact gattggaaca ctaccgttag ttacaagaga taatgcatcc tggtggccag
      121 ggaaccggta atcgaagaat accattttat gcttatttat cactcctgaa ttgtcgtcag
      181 acttgaaggc ataggatact acatgagctg ttgtggctgc aggaaccata gccggcgaga
      241 ccggcctgaa atatgcaaca atagtcaaat tctcgcagaa catgctccct ggactgatag
      301 catttgcaat ttctattttc tgtgcctata gaggcagggg cgcaaatgaa aagggacagc
      361 attactgtct gcacctccag gattatgtca gcccctgact ttttggcgaa caggagcaaa
      421 atcaaagcac acggaatctt aatgctcatt gtatgtgcag atcatatgta agaacaccct
      481 gcaaaataac gacattctgc acgaaattag atacgggtac tgaaatgagt ttcaggtaaa
      541 cgcactaatc tgtttcttac tctctcagta taagcttaat tggcaccctg atgaaacgtg
      601 ccctgtattt ttcaacatcg ctaaagacat ccccggggtt taacgaattt acacaatatg
      661 acaccagcaa ccggttgttt tcaatgtatt gcatatgcgc cagagcattg tatgtaaaga
      721 cccgtttact cgtatcagct tcggtctcgg cagtatgata gatgaatttc ttgttatgaa
      781 atcccctgtc cggaaaatct gaaacatagg tatatatatc tgcccctgcc ctttcttgtg
      841 tcagaagtat gtatttattc ctgtgcctga agatgctgaa ttgttcagaa actgatatgt
      901 caatatccag aggagctgcc ctgctgatat ccctttccca cccggcccct gtggagaatt
      961 cccaatcgcg gtacagcgtg ctgctattaa aaggcacacg ggctgcatgt atttcagctt
     1021 tagctgaaag gtttctcgtt ccgaagatat aaacgtaccc gttatcgacc atgacacagt
     1081 tgccgaaatc gatctgactt acagggtaat caatctcagt gcggtgcgtt tcaagatgcc
     1141 tgaaatcagg atagctgaat gacaacagat cgatgtgaga tatcctgaac gaaaaaatca
     1201 tttctgccat atcaacggtt tcgtcatctg cgccttccgg aagtccaaaa tttatgaccg
     1261 cagaagtatc attcaccagg ctgagggcaa agacatagat agtatcattt ctgatgaagc
     1321 catgaccagg ccagtaccag cggtatgtgg aatcaccagg ctcattcacg ggttccatta
     1381 gtgtcagggg agcatcaatt gttcccctga caattgccat ggcggcggtt ttctccttat
     1441 tgacaagatt aaacgcattc cgcagcatcg gggtgctggt atcacgggcc ccgctcctga
     1501 cctccccaat gaaacaatca cctaacagga atatggaaga gccatcaggc aacgggaagg
     1561 agaacaggcc atcagcaccc gaaatacccc ctgagtctga atagaagaca gatctgaatt
     1621 cattgtcagg tgatacggct tctgcccctg ttcctgatcc cggcttgcat gatgggctga
     1681 aaagcaatac gcctaacatt cctatggtga atgctttgct ttggatgttc cctggttttg
     1741 tcatctgatc atcttttaaa atatgcatca taagtttatg taattcttct atttgtacct
     1801 gactttaatg aaacgaggat gataaatggt ggcatctttc cggatatctt cccagaagtt
     1861 taatgtgatg gtattgtagc tgacaaggag ttcaccgggt tttgaaagat gataatgtgc
     1921 cttggcatta tatggcagca aacctttttc agcatactcg gggcaggtgt acacttcatt
     1981 gatctctccg aatggcccta cggggctgtt gccgacacga atgcctattt tatcagatat
     2041 gcccattacg gtaaaagtca gaaggtacct gccgtcacct gtaggtgtca cactgagctc
     2101 gtttgagata tcatctgtta ttggcgcgat gttctctttt ccctctaccc actgttctcc
     2161 gttccagaag gcccatctgt cagtctcttc aaagtcagca ggcctggttc gtgctgccag
     2221 aagcgatttc ttcccctccg ctattccgta aatataaacg tacccgtcgg ggttgggagc
     2281 tcctgccttt ctggtgttca caaaaatccc gcttccaaaa tatgttcttc caagcaccgg
     2341 atgaataaag cccaggcccg taggtatctg ttcatagacc tttattcctt ctggtgcagg
     2401 attctttatt ttcaggaggg caacattggt agcttcgaaa tcaaagacat ttggacctgt
     2461 tttgtgcaca tgatatgcaa agatgtaaag tgaatttttt ctggcatgat tgatgaatcc
     2521 gtcgcccagc cagaagtact ctccttcggt gctgttactg ttatcaggag tgaaataggt
     2581 ggccgggctg ccgtcactgt tttttttgta ctcaaaaaca attgcctgcc tctctggtat
     2641 gatacctgac atccaggcag ttgtattgtt aaccatgacg ttgccggctt gcggcacatt
     2701 atctttaacc tcaccgatat atgtatcgct gaaaatgaag agagtcttct tgtctttgtt
     2761 ctgctgatct tcaatgccat caagaggtat ggaaaaaata ccatcggctc caaaccagcc
     2821 ggaggtccgc tccatcaggg cggtccattc cggtgcaggc tcactggtaa atgtcatcgg
     2881 ctgcagggac tcagactggt tcctggcagc atcgctgcct ggtttcggat tgcagccgct
     2941 gactatcacc acaactgata tcaccgcgag gatcctgctt ttcataattg taagtatttc
     3001 aagagtcatt tgtcctgagt tattccttaa cgtattttat gatcgcatcc ttccagacca
     3061 ggtatccttt cccgttcagg tgaagtccgt caatgctgta tgcaggattc aacttgtcat
     3121 tctcaagcct gaagagagtg aacagatcaa tatataccag cctgttctct gacgcgatct
     3181 gttttaaaag ttcgttaatc cttataatat c
//
LOCUS       DAOPBK010000237         3162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_24376_length_3162_cov_5.763759, whole genome shotgun sequence.
ACCESSION   DAOPBK010000237 DAOPBK010000000
VERSION     DAOPBK010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24376_length_3162_cov_5.763759"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PLA17_10590"
     CDS             <1..1141
                     /locus_tag="PLA17_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HPK85901.1"
                     /translation="KNLAIILACLTFMAISCNRNASHDYMVVGYVAGYRNFDFSQINA
                     SSLTHINYAFANIINGEVMFGSEGIDNTDMNADDIKTLTALKSVNPDLRILVSVGGWG
                     WSGNFSDAALTDSSRNRFAASAARFIREYSLDGIDIDWEYPNHPGAGNTYRAEDVHNF
                     TLMLECVRKHIDSLAAAEGRKEKYLLTIATGASELYAANTELGPLSQYLDFINIMTYD
                     FHHGASYQTGHHANLHLSSLDAPDGDATDRAVELHLKAGILPAKLNIGIPFYGRVWRG
                     VEPVNNGLYREATTTGAGMPYAEVLEALANPAFTRFYDSSAASPFLWNESDSVFISYE
                     DVTSIAARMKYVREKGLGGVMFWEYTEDVGGKLLQAITDGLNDRQ"
     gene            complement(1484..2386)
                     /locus_tag="PLA17_10595"
     CDS             complement(1484..2386)
                     /locus_tag="PLA17_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPK85902.1"
                     /translation="MNYITSHTGEFAALAVAIFWTVTALAFESASRKVGSLTVNILRL
                     IMAFFFLGIYTLITNGHFLPLDASIHNWLWLGLSGIVGLVLGDYFLFRSYPLIGSRFA
                     MLIMTLAPPLAAIFGYFILGESLNLMQMTGMIIVIFGISLAIFNKPVSGERLSIKISP
                     AGLLFAFIGAIGQGLGIVLSKYGMEEYDAFASTQIRIIAGIAGFSILITLLRRWGNVA
                     AALKNPPAMKALTVGAIFGPFLGISFSLLAVRHTQAGIASTIMAIVPVLILAPSAWIY
                     KEKITAVEIAGAIISVAGVAFFFI"
     gene            2527..2838
                     /locus_tag="PLA17_10600"
     CDS             2527..2838
                     /locus_tag="PLA17_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85903.1"
                     /translation="MNAKTKAIVSHLFVIGWLIALIVNSSKKEQLASFYIRQNLGFIV
                     VWVALEVLRILPIVGPVIRVVGGVLLFIGWLMSLIWSIQGEQKPVPWLGEQFQAWFRG
                     F"
     gene            2885..>3162
                     /locus_tag="PLA17_10605"
     CDS             2885..>3162
                     /locus_tag="PLA17_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPK85904.1"
                     /translation="MAVSFFSGCSEPERGKPNIILIIADDLGYGDTGPYGQSKIETPN
                     IDRLAAEGLRFTHFYAGSPVCAPSRCVLLTGMHTGHSQVRGNDEWEERG"
BASE COUNT          819 a          745 c          836 g          762 t
ORIGIN      
        1 aaaaaacctt gccatcatcc ttgcctgcct cactttcatg gccatatcat gcaacaggaa
       61 cgcctctcat gactatatgg ttgttggtta tgtggccggc tacaggaact tcgatttttc
      121 acagatcaat gcttcaagtc tgacccacat caactatgcc ttcgcaaata tcatcaatgg
      181 tgaggtgatg ttcggaagcg agggcattga caatactgac atgaatgcag atgatataaa
      241 gacccttacc gcactgaaga gtgtcaatcc tgatctcaga atcctcgttt ccgtcggggg
      301 gtgggggtgg tccggcaact tctccgatgc agcccttact gactcttcac gaaatcgctt
      361 cgcggcgagt gccgcaaggt tcatcaggga atattccctt gacgggattg acattgactg
      421 ggaatacccg aatcatccgg gtgccgggaa tacgtacagg gctgaggatg tacacaattt
      481 cacactgatg cttgagtgtg tcaggaaaca cattgattcc ctggcagcgg cggaggggag
      541 gaaggagaaa tacctcctga ccattgccac gggcgccagt gagctctatg ctgcaaatac
      601 ggaactcggc ccgctatcac aatatctcga tttcatcaat atcatgacct atgattttca
      661 tcacggcgcc agctaccaga cgggtcatca tgccaacctg cacctttcat cccttgatgc
      721 tcctgacggc gatgccaccg acagggcagt ggagctgcac ctgaaagcgg ggatactgcc
      781 ggcgaagttg aatataggga ttccgttcta cggaagagta tggagagggg tggagccggt
      841 taacaacggc ctctaccgcg aagccaccac cacaggggcc ggcatgccgt acgccgaagt
      901 gctcgaagcc cttgccaacc cggcctttac cagattctac gactcatcgg ccgcttcacc
      961 attcctctgg aatgagagtg actcggtctt tatatcctac gaggatgtaa cctccatcgc
     1021 tgccaggatg aagtatgtca gggaaaaagg gctgggtggc gtcatgttct gggagtacac
     1081 ggaagatgtc ggcgggaagc tcctgcaggc aattaccgac ggactaaatg acaggcaata
     1141 gctgacgggc tgaatgacaa ataataaccg acggactgaa tgacaggcaa taactgacgg
     1201 actgaatacc agacaatagt aaaggagagg gcagagtatc aacctattct aagggattct
     1261 gcaagctgaa ctccagacaa tagtattaaa agtaataatc aaggggctga tcgtagtaaa
     1321 gctttatttg agaaactata ttttccacta actcagctga ttattgaggg gattggaaat
     1381 caaaaatcta tatgtcaaga attgtattgt ctgctcaatc accttattat tgggggggcg
     1441 agtttcgcag agcttgattg ctgtttcaca gcagaccggg taatcagata aagaaaaatg
     1501 cgacgcctgc cacgctgatg atcgcaccgg caatctcaac agcagtgatt ttttccttgt
     1561 agatccatgc tgagggagca aggatcagca ctggcacaat tgccattatt gttgatgcaa
     1621 tgccggcctg ggtgtgcctg actgcaagaa gtgaaaatga tatccccaga aaagggccaa
     1681 aaatcgcacc caccgtcagc gctttcatgg ctggcggatt tttcagcgct gctgccacat
     1741 ttccccatcg cctgagtaaa gtaatcaata ttgagaatcc ggcaatccct gcaattattc
     1801 gtatctgtgt ggaagcgaat gcatcatact cctccatgcc gtacttgctg agcactattc
     1861 caaggccctg gcctatggct ccgataaatg caaagagcaa tccggccggt gaaatcttta
     1921 tggagagtct ttctccgctg acgggcttat tgaagatggc caggctgatg ccgaagatca
     1981 ctatgatcat gccggtcatc tgcatcaggt tcagcgattc acccaggata aaatagccga
     2041 agattgctgc cagcggtggc gccagggtca tgatcagcat cgcgaacctt gaaccgatga
     2101 gcggatagga tcggaacagg aaataatcgc caagaaccag tcccactatc cccgagagtc
     2161 ccagccagag ccagttgtgg atgctggcat caagaggcag gaaatgacca ttggtaatga
     2221 gggtatagat tcccaggaag aagaaagcca tgatcagcct gaggatgttg accgtaaggg
     2281 agccgacttt gcggctggct gactcgaatg ccagcgcggt aacggtccag aagattgcca
     2341 cagccagagc ggcaaattcc ccggtgtgtg acgtaatgta gttcatgccc ggaaatatca
     2401 gcgcacaaaa atatacaata ctccggtccc tgttccttat cgggctgttt tatacgggat
     2461 tttttccttt aaccggttat atgacggttt tatggttatt tttggataaa cctaaaacag
     2521 aatatcatga atgcaaagac aaaggccatt gtttcccacc tgtttgtgat aggttggttg
     2581 attgctctca ttgtcaattc gagtaagaag gaacagttgg ccagttttta tatcaggcag
     2641 aatctcggat tcatagtagt atgggtggca ttggaggttc tccggatact gcccattgtc
     2701 ggtccggtca tccgggttgt tggcggggtc cttctgttta ttggctggct catgagtctc
     2761 atctggtcaa tacagggaga gcagaagccg gtaccatggt tgggtgaaca gttccaggcc
     2821 tggttcaggg gattctgatc tgatgagcgg aagagtacat atatggtctg ttggctttct
     2881 gctgatggcg gtctcatttt tttccggatg cagtgagccg gagagaggga agcccaacat
     2941 catactgatt attgccgacg acctgggata cggtgacacc ggcccctacg ggcagtctaa
     3001 aattgagact cccaatattg acaggctggc ggccgaggga ttacggttca ctcatttcta
     3061 tgcaggatcg ccggtatgtg ccccttcacg atgtgttctc ctgacaggta tgcacaccgg
     3121 tcactcacag gtaaggggta acgacgagtg ggaagagagg gg
//
LOCUS       DAOPBK010000238         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_24971_length_3101_cov_5.677610, whole genome shotgun sequence.
ACCESSION   DAOPBK010000238 DAOPBK010000000
VERSION     DAOPBK010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24971_length_3101_cov_5.677610"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1358)
                     /locus_tag="PLA17_10610"
     CDS             complement(219..1358)
                     /locus_tag="PLA17_10610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK85905.1"
                     /translation="MRIVICGPAHPYRGGIAAYNERLAQQLLHEGDAAAIYTFKLQYP
                     SFLFPGKTQYDHGYAPEGVTITRAINSVNPLNWFNIGLRLRRIRPDLVIMRYWLPFLA
                     PALGFIARLVKSNRHTMVVTIFDNVVPHEKRIGDTMLTRFFVKSIDGALVMTRAVEND
                     LRKFSADMPCVISPHPLFDNYSVPVSRDEAIAHLKLPQADKYILFFGFIREYKGLDLL
                     LRAMAEKCVRDLGVKLIVAGEFYESKKPYFDLVSQLGTGDRIIFHSHFISDDEVKYYF
                     SAASLVVQPYKSATQSGVTQIAYHFNKPMIVTNVGGLAEIVPDGVSGYVVEPDPVAIA
                     GAICDFFSGDEKRFEAGIEKKKKEYSWSRLTEALHSLVREVQRAR"
     gene            complement(1358..2593)
                     /locus_tag="PLA17_10615"
     CDS             complement(1358..2593)
                     /locus_tag="PLA17_10615"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK85906.1"
                     /translation="MEKKVKVGLIRETKTPPDRRVPLTPSQVVELKEKYPNVEFYVQP
                     SNIRCFTDDEYEYLKIPMREDISNCDILMGVKEVEKSALIPGKTYLFFAHVAKKQEHN
                     LEMLREILKRKVRIIDYEYLTTDKGLRVVAFGRWAGIVGAYNALRARGIKTNRFNLKP
                     AHQCHDLNEMWAGLKLIQLKPGLKILVTGEGRVSNGALETLFNCHNLMKVSTQDFLSK
                     EFDVPVVCQVGPQDYTKHHDGRPFDFNYFMKHPEEYRNDFLKFTKVTDILIAGHFWDP
                     KSPIFFTKEDVEKPDFRISVIGDVSCDIDGPIPTTLRATTIADPFYSYNRKKHCEEKA
                     FSNPDNITVMSIDNLPGELPRDASGDFGRQLIDNVLHDLFTGHDSPMIQRATITSGGR
                     IEPQYGHLKSWVWPEGEKS"
     gene            2705..3101
                     /gene="ssrA"
                     /locus_tag="PLA17_10620"
     tmRNA           2705..3101
                     /gene="ssrA"
                     /locus_tag="PLA17_10620"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          712 a          836 c          746 g          807 t
ORIGIN      
        1 cctgctgact gccggctcca cttctgactg ctgctggcag cttctgcctg acgctaatcc
       61 cccgctgcta cctgctgact gccggttgcg ggctgctgat tcccaactgc ctgctactgc
      121 gggttgctga ctgcctgctg ctggttacta atcgactgat actgactcct gactccctgc
      181 tgctgagagc tatctgccgg ctaactgccg gctgattatt acctcgcgcg ctgaacctct
      241 ctcacgaggc tgtggagcgc ctctgtaagc ctgctccacg agtactcctt tttcttcttt
      301 tctattcctg cttcaaacct cttctcatcg ccgctgaaga aatcgcatat agctccggca
      361 attgcaacag gatcaggctc aacaacatat ccactgactc catccggtac tatctcagcc
      421 agtcccccca cattcgtaac aatcatcggc ttgttgaagt gatatgctat ctgagtaaca
      481 ccgctctggg tggcactctt gtaaggttgc accaccagcg aggctgctga gaagtaatac
      541 ttgacctcat catcactgat gaagtgggag tggaagatga ttctgtcgcc tgttccgagc
      601 tggctgacga gatcgaagta aggttttttg ctctcgtaga actcacctgc aacgataagc
      661 ttcacgccga ggtcccggac acacttctca gccatggccc tcagaagcag gtcaagcccc
      721 ttgtactccc ttataaaccc gaagaagagg atgtatttgt cagcctgagg cagtttaaga
      781 tgagcgattg cctcatcccg tgataccgga acgctgtagt tgtcaaacag gggatgggga
      841 gagatcacgc agggcatatc agcagaaaac ttccggagat cattctccac agcccttgtc
      901 atcaccaatg cgccatctat gctcttcaca aagaacctgg tgagcatggt gtcaccgatc
      961 cgtttctcgt ggggtaccac attatcaaaa atggtgacga ccatggtgtg cctgttgctt
     1021 tttacaagcc gtgcaatgaa acccagcgcc ggggcgagga agggaagcca gtagcgcatt
     1081 attacaaggt ccggacggat gcgtctcaga cgaagcccga tattgaacca gttcagcggg
     1141 ttgaccgagt taatggctct tgtaatggta actccctccg gggcataccc atgatcatac
     1201 tgtgtcttcc ccgggaacag aaaagaggga tactgaagct tgaacgtata aatggccgca
     1261 gcatcgcctt cgtgaagcag ttgttgtgcc agcctctcgt tgtaagcggc tatcccgcca
     1321 cggtatggat gggcggggcc acagataacg attcgcatca tgacttctca ccttcaggcc
     1381 atacccagct tttcaggtgc ccgtattgcg gttcaatcct cccgcctgag gttatcgtgg
     1441 cgcgctgaat cataggtgaa tcatggcctg taaagagatc atgcaacaca ttatcaatca
     1501 attgtctgcc gaaatctccc gaggcatccc tcggcagctc tccgggaaga ttgtcaattg
     1561 acatgactgt gatattgtca ggattgctga atgccttctc ctcgcaatgc ttcttccggt
     1621 tatagctgta gaacgggtct gcaatggtag ttgcccgcag ggttgtgggt atcggaccat
     1681 ctatatcaca gctgacgtca ccaatgaccg aaatcctgaa atcaggcttt tccacatcct
     1741 ccttcgtgaa gaatatcggt gacttcggat cccagaagtg tccggcaatc agaatatccg
     1801 tcaccttcgt gaatttcagg aaatcattcc tgtactcctc aggatgcttc atgaaatagt
     1861 taaagtcaaa cggacggcca tcatgatgct tcgtataatc ctgcggtccc acctggcaga
     1921 caacgggaac atcgaactcc ttcgacagga aatcttgggt gcttaccttc atcaggttat
     1981 ggcagttgaa cagagtctcc agagcgccat tagataccct cccctcgccg gtaaccagga
     2041 tcttcagtcc cggcttgagc tgtatcagct tcagtccggc ccacatctca ttcaggtcat
     2101 gacactggtg agctggtttc agattgaatc tgttcgtttt tatacccctg gctctcaggg
     2161 cattatatgc acccactata cctgcccacc ggccgaaggc caccactctc aggcccttgt
     2221 ccgtagtcag gtattcgtag tcaataattc tcaccttcct tttgagtatc tccctgagca
     2281 tctcgaggtt gtgttcctgt ttcttggcca catgggcaaa gaagaggtaa gtcttcccgg
     2341 ggatcaatgc actcttttcc acctccttga ctcccatgag aatatcgcag ttactgatat
     2401 cttcccgcat gggaatcttc aggtattcgt attcgtcgtc agtaaagcac ctgatatttg
     2461 acggctggac ataaaactcc acgtttggat atttttcttt cagttcaacc acctgtgacg
     2521 gtgtcagtgg cactcttctg tctggcggtg tttttgtctc acgaatgagc cctaccttca
     2581 ctttcttttc catgtgaaaa caattttgta ttgcggtgaa gataagaaat cggcccggtt
     2641 tttgcgagga cttttgtcag ttcaacgtat aaatgactaa ttttgcgttg taaaatatat
     2701 caacggggct gacctggttt tgacagcaag ccgtggtggt atgtaagcat gcagagtagg
     2761 gagacgcatc tctataactt gtgtctcaaa acaataactg gcgagtataa ctacgctctt
     2821 gctgcgtagt ctgccgacag gcggattatg cttaatcccg gcacaaggtg ttgggacgag
     2881 acgtttcccg ggcggtgagg ccctgtacca acccgataag gaagcgcacg caactcaggg
     2941 atagcccgcg gcttctccgg cttaacggtg aaataacagg agataaggag tgattcggct
     3001 gcctgccacc tgatcacctc ccgacaaata aggcaggata agcatgtaga aagcatatgg
     3061 ctgccttgcc tggacgcggg ttcgactccc gccagctcca c
//
LOCUS       DAOPBK010000239         3026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_25745_length_3026_cov_4.664423, whole genome shotgun sequence.
ACCESSION   DAOPBK010000239 DAOPBK010000000
VERSION     DAOPBK010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25745_length_3026_cov_4.664423"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..912
                     /locus_tag="PLA17_10625"
     CDS             310..912
                     /locus_tag="PLA17_10625"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPK85907.1"
                     /translation="MFSGIVEEAAPVVRIEQEKDNIHITMRCSFAHELKIDQSVSHNG
                     VCLTVVKTDGDTYTVTAIRETLEKSNLGLLKPGDKVNLERSTRLDGRLDGHMVQGHVD
                     LTATCTSVREAEGSWYYSFEYEPTSPEHITVEKGSVSVNGVSLTVVNSKEKSFDVAII
                     PFTWEVTNFHQIKAGTVVNIEFDIIGKYIAKIVRQVLAKD"
     gene            998..1615
                     /locus_tag="PLA17_10630"
     CDS             998..1615
                     /locus_tag="PLA17_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85908.1"
                     /translation="MNFLEFRTRMFPLACFNVNQVYAWQPDFNRNNLVRWTSKGLIIR
                     LKQGYYTFPEYLSMPDYPYYFANRIYFPSYISLHTALASYGVIPEGVTQITSVTSLKT
                     ASFVNPAGTYVYKSVREDLMFGYITRPLADGRTLLFASPEKAIIDLLYLYPFYNTARE
                     MEELRFDDYFLHEELNIDLLSEYSSRIRSRALDRRVRLFLSSYEL"
     gene            1612..2394
                     /locus_tag="PLA17_10635"
     CDS             1612..2394
                     /locus_tag="PLA17_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPK85909.1"
                     /translation="MIRIDDLKNFYPPLVRENPMHNRYIIKEYIQLLILDYLSTTQWV
                     KKISLIGGTCLRLLKGIDRFSEDLDFDCKELSKEEFVLMTDDIISLLRRNGFQAEPRD
                     RENIRSKAFRRNVHFPELLFNFGLTGHREERFLITVKAEDQKFAYIPVLANIRLNGFF
                     FPFPVPPDAILCSMKVSAMISRQKGRDFYDVMYLLSQINPDYLFLSSKHSISGPDDLK
                     NSVTGMLKNIDLKMKSRDFEHLLFNRKNSVRILRIAEFFNEI"
     gene            2426..>3026
                     /locus_tag="PLA17_10640"
     CDS             2426..>3026
                     /locus_tag="PLA17_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85910.1"
                     /translation="MNNRKIIVAITGASGAIYAVKLLERLRKLPESRSGLAGPPELHG
                     LPGSPELSGLHGLTDSHGQAGLHKLPGLHGLADSPGLPESPEQSGLHELTGLPESPGP
                     HESHRLDKSPELHGQGMQSHGQTNPSHPLQEPLAEVAVILTANAEEIMLYETGAGYTP
                     SGNEKLWSNNDFNAPFASGSSTYDTMIICPASMGMVGRIA"
BASE COUNT          805 a          742 c          676 g          803 t
ORIGIN      
        1 attgattcag aacaatcaaa ggactcagaa atgagaatgc agttaatgtt gattttagcc
       61 tgtaaagcaa acagatatgt tttcaactat aacaagtttt gaccatttcc tgtaatcatg
      121 ccttcaacat tattcaattt ggccatttct tgtattcata tctacaactt tatcctgttt
      181 aatcatttct tgtagttata ccttcaactt tatctctttt gactgttttc tgtaatcatg
      241 tttacaactc ttgcaatggt tactctcttt tgcatttcct atctttgccc ataacaaatc
      301 actgacatta tgttttcggg tatagtagag gaagctgcac cggtggtgcg aatagaacag
      361 gagaaggata atattcacat aaccatgcga tgctcattcg cccatgagct gaagatagac
      421 cagagcgttt cacacaacgg cgtgtgcctc acggtggtga agaccgacgg cgacacctac
      481 accgtcaccg ccatccgcga gactcttgag aagtccaacc tcgggttgct taagccgggc
      541 gacaaggtca atcttgagcg cagcacccgc ctcgacgggc gtctcgacgg ccacatggta
      601 cagggtcatg ttgatctcac cgcaacatgc acctctgtgc gcgaggccga agggtcatgg
      661 tactattcct ttgaatatga accaacttcg cccgagcata tcaccgtgga gaagggctcg
      721 gtcagcgtga acggagtgag ccttactgtt gtcaactcaa aggagaagtc gtttgatgtg
      781 gcaatcatcc ccttcacctg ggaggtgaca aactttcacc agataaaggc tggcacggta
      841 gtcaacatcg agtttgacat catcggaaaa tatattgcaa agatcgtcag acaggtgctg
      901 gccaaagact aaagtccgga agaaaaacag ctcctaataa tcttgcattt atttctgttt
      961 tgcactatat ttgttgcata acggaaatgt aactgtaatg aattttctcg aattcagaac
     1021 acgcatgttt cctctggctt gctttaatgt aaaccaggtc tatgcatggc agcctgattt
     1081 caacaggaac aacctggtac gctggaccag caagggattg attatcagat tgaagcaggg
     1141 ttattacact ttccccgaat atctgagtat gcctgattac ccgtattact ttgcaaaccg
     1201 catctacttt ccctcataca tcagcctgca tactgcactg gcttcttacg gagttattcc
     1261 tgaaggtgtc acccaaatta ccagtgttac ctctttaaaa actgcttcgt ttgtcaaccc
     1321 ggcaggaact tatgtttaca aatcggtcag ggaagatctt atgttcggat acatcactag
     1381 gccgttggcc gatggccgga cactgttgtt tgcctcgccg gaaaaggcaa tcatcgatct
     1441 gctttacctt taccccttct ataacactgc cagggagatg gaagagctcc ggtttgatga
     1501 ttatttcctt catgaagaac tcaacattga tttgctttcg gaatactcct ccaggatcag
     1561 gagccgggca ttggacagga gggtcagact ctttttaagc agctatgaat tatgatacga
     1621 attgatgacc tgaaaaattt ctatccccct cttgtgcggg aaaacccgat gcacaacagg
     1681 tatattataa aggagtatat ccaacttttg attcttgatt acctgtcgac aacccaatgg
     1741 gtaaagaaga tttcacttat tggaggtaca tgcctgcgac ttctgaaggg tattgacagg
     1801 ttttccgagg atcttgattt cgactgcaaa gaactgtcga aggaggagtt cgtgctgatg
     1861 actgatgaca tcatcagtct gctgcgcaga aacggatttc aagccgaacc cagagacagg
     1921 gaaaatataa ggtcaaaggc tttccggaga aatgttcatt tcccggagct gttgttcaat
     1981 tttggcctga cgggacaccg ggaggagcgt ttccttataa cggtcaaagc agaggatcag
     2041 aagttcgctt acattcctgt tctggcaaac atcaggctta acggattctt cttccccttc
     2101 ccggttcctc cggacgcaat actctgttca atgaaggttt cagcaatgat cagccggcaa
     2161 aagggtcgtg acttctacga tgtaatgtat cttctgagcc agataaaccc ggactattta
     2221 tttctcagtt caaaacattc tatttctggt cctgatgatc ttaagaattc tgtgacaggc
     2281 atgctgaaga acattgacct gaagatgaag agcagggact ttgagcatct ccttttcaac
     2341 aggaaaaaca gtgtgcgcat attgcgcatt gcagaatttt tcaatgagat ataactattg
     2401 atatctttgc ctggctaagt agcttatgaa taaccgaaaa ataattgttg ccatcacggg
     2461 cgccagcggc gcgatatacg ccgtgaagct gctggagcgg ctgcggaaac tgcctgagtc
     2521 gcgttccgga ctggccggac ctcccgaact tcacgggcta cccggatctc ctgaactatc
     2581 cgggctccac ggactaactg attctcacgg acaagccggg ctccacaaat tacctggtct
     2641 tcacggacta gctgattctc ccggactacc cgaatctccc gaacaatccg gacttcacga
     2701 actaaccgga ctacccgaat ctcccgggcc tcacgaatct cacagactag acaaatctcc
     2761 cgaacttcac gggcaaggca tgcaaagtca cgggcagaca aacccgtcgc acccccttca
     2821 ggaaccgctg gcagaggtgg ccgttatcct cactgcaaat gcagaggaga tcatgctcta
     2881 cgagaccggt gccggttaca ctccctcagg caacgagaag ctctggagca acaacgactt
     2941 caacgcaccg ttcgcttcgg gatcatcaac ttatgacacc atgatcatct gtcctgcttc
     3001 aatgggcatg gtgggccgca tcgccc
//
LOCUS       DAOPBK010000240         3010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_25896_length_3010_cov_4.381049, whole genome shotgun sequence.
ACCESSION   DAOPBK010000240 DAOPBK010000000
VERSION     DAOPBK010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25896_length_3010_cov_4.381049"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLA17_10645"
     CDS             <1..412
                     /locus_tag="PLA17_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HPK85911.1"
                     /translation="WYNLTTAILIILFTYFYTAVTINPVQMAEDMKKNGGFIPGIKPG
                     RKTVEFLDTIMSRITLPGSIFLAIIAILPAFAVNFGISAEFAQFFGGTSLLILVGVVL
                     DTLQQIESHLLMRHYDGLMKSGRIKGRTGAVTAI"
     gene            430..1206
                     /gene="map"
                     /locus_tag="PLA17_10650"
     CDS             430..1206
                     /gene="map"
                     /locus_tag="PLA17_10650"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPK85912.1"
                     /translation="MIYVKTDAEVELLREANQLVSKTLAEVAVHIRPGVTTLRLDAIA
                     EEFIRSHGALPAFKGYAGFPNTLCTSVNDEVVHGIPSDYALMEGDIISVDCGVILNGW
                     YGDSAFTFPVGEVKPEILRLLDFTRASLEEGVRQAIAGNRIGDISHAVQSKAEGGGYS
                     VVRTLVGHGIGKHLHEGPEVPNFGRRGSGTRLERNMVICIEPMINMGTKYTSTDSDGW
                     TVRTGDGKPSAHFEYAVAAREGKPDLLSTFEYIEEVLTKR"
     gene            1211..1429
                     /gene="infA"
                     /locus_tag="PLA17_10655"
     CDS             1211..1429
                     /gene="infA"
                     /locus_tag="PLA17_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPK85913.1"
                     /translation="MAKQPSIEIDGTIIEALSNAMFRVRLENGHIITAHISGKMRMHY
                     IKILPGDKVKVEMSPYDLTKGRITFRFK"
     gene            1444..1560
                     /gene="ykgO"
                     /locus_tag="PLA17_10660"
     CDS             1444..1560
                     /gene="ykgO"
                     /locus_tag="PLA17_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HPK85914.1"
                     /translation="MKVRASIKKRSADCKIVRRHGRLYVINKKNPKFKQRQG"
     gene            1598..1975
                     /gene="rpsM"
                     /locus_tag="PLA17_10665"
     CDS             1598..1975
                     /gene="rpsM"
                     /locus_tag="PLA17_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPK85915.1"
                     /translation="MARIIGVDLPRNKQGEIALTHIYGVGRSTAQKVLDKAGVDRHMK
                     VEDWNDDNINAIRQILSENYKLEGELRSETQMNIKRLMDIGCYRGIRHRIGLPVRGQS
                     TKNNARTRKGRKKTVANKKKATK"
     gene            1984..2373
                     /gene="rpsK"
                     /locus_tag="PLA17_10670"
     CDS             1984..2373
                     /gene="rpsK"
                     /locus_tag="PLA17_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPK85916.1"
                     /translation="MAKKTGTLKKKVVKVEPVGQAHIHSSFNNIIISLTNSTGQVISW
                     ASAGKMGFKGSKKNTPYAAQMAAEECTKVAFDLGLRKVKVFVKGPGSGRESAIRAISN
                     SGVEVTEIIDITPIPHNGCRPPARRRV"
     gene            2418..>3010
                     /gene="rpsD"
                     /locus_tag="PLA17_10675"
     CDS             2418..>3010
                     /gene="rpsD"
                     /locus_tag="PLA17_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPK85917.1"
                     /translation="MARYTGPRSRIARKFGEPIFGPDKYLDRKNFPPGQHGLTKKRKK
                     TSEYGIQLREKQKVKYTYGLLERQFRNTFEKAVAASGVTGLVLLQMLESRLDNVVYRL
                     GIAPTRPAARQLVTHRHITINGNVVNIPSMMLRPGDIIGVREKSKSLEVITECLGANR
                     RSKMTWLEWDDTQMAGKYMSVPERTEIPEDIKEQLIV"
BASE COUNT          868 a          706 c          775 g          661 t
ORIGIN      
        1 ctggtataac ctgacaacag caatactcat catcctcttc acctatttct ataccgcggt
       61 taccattaac ccggtgcaaa tggctgaaga catgaagaag aacggtggtt tcattccagg
      121 catcaagccg ggacgcaaga cggttgaatt ccttgatacc atcatgtcac gaattaccct
      181 tccgggttca atattcctcg caataatagc catactccct gcttttgccg tgaacttcgg
      241 tatcagcgct gagtttgcac agttcttcgg aggcacctca cttctcattc tcgtgggtgt
      301 tgtgcttgat actcttcagc agatcgaaag ccatctgctc atgcgccact atgacggcct
      361 catgaaatca ggccgtatta agggcaggac cggagcggtg acggctattt aatgcgattt
      421 cgttctttga tgatctatgt taagacagac gcggaagtag agttactgag agaagccaac
      481 cagctggttt caaaaactct tgcggaagta gccgtgcata tcagaccggg ggtgacaacc
      541 ctccgtcttg acgctatagc tgaagagttc atccgttcac acggagcact ccctgccttt
      601 aagggatatg ccggattccc gaacactctc tgcacatctg taaatgacga ggtggtacac
      661 ggaataccgt cagactatgc actcatggag ggcgacataa tatcggttga ctgcggtgtg
      721 atactgaacg gctggtacgg tgacagcgcc ttcaccttcc cggtgggaga ggtgaagcct
      781 gaaatacttc gtcttctcga tttcacccgg gcttctcttg aggagggtgt gaggcaggcc
      841 attgccggta accgcatagg agacatctcg catgcagtac agtcaaaggc cgagggcgga
      901 ggatattcag tagtgcgcac gctggtgggg cacggaatcg ggaagcacct tcacgagggc
      961 cctgaggtgc ctaacttcgg gagacgcggc tctggcacga gacttgaaag gaatatggtc
     1021 atctgcatag aaccgatgat caatatgggt accaaataca caagcactga cagtgacggg
     1081 tggacagtca ggacaggtga cggcaagccg tcggcacatt ttgaatatgc tgttgcggcc
     1141 agggagggga agcccgatct gctctcaaca tttgaatata tagaagaagt attaacaaag
     1201 aggtaagtat atggccaaac aaccatcaat agagattgac ggaacgatca tagaagcgct
     1261 ttcaaacgcg atgttcaggg tcaggctcga aaacggacat atcatcaccg cacatatctc
     1321 cgggaagatg aggatgcact atatcaagat tctccccggt gacaaggtga aggttgaaat
     1381 gtcgccctat gatcttacca aaggaagaat aacgttcagg tttaaataga aaacaacagt
     1441 gcgatgaaag taagagcatc aatcaagaag cgctcggctg actgcaagat agtaaggcgt
     1501 cacgggcgtc tgtatgtaat aaataagaaa aatcccaagt tcaagcagag acagggataa
     1561 atttgattat ctttgcactt caaatttaaa gaggtatatg gcacgtatta ttggggtaga
     1621 cctaccaagg aacaagcagg gtgaaatagc tctcacccac atctatggcg ttgggcgcag
     1681 tactgcacag aaggtgcttg acaaagccgg cgttgaccgt cacatgaagg tagaggattg
     1741 gaatgatgac aacatcaatg ctatccgtca gattctctcc gagaattaca agctggaggg
     1801 tgagcttcgt tccgaaacgc agatgaacat caagcggctg atggatattg gttgctatcg
     1861 cggcattcgt caccgtatcg gactgccggt aaggggccag agcacgaaga ataacgcccg
     1921 tacccgcaaa ggacgcaaga agactgtggc aaacaagaaa aaagctacta aataatgtaa
     1981 tatatggcaa agaagactgg gacattaaag aagaaggtcg taaaggttga acccgtagga
     2041 caggctcaca ttcattcgtc attcaacaat atcatcataa gtcttacaaa cagcaccggc
     2101 caggttatat cctgggcctc ggcaggcaag atgggattca agggttcgaa gaagaacact
     2161 ccctatgccg cacagatggc agctgaagag tgcaccaagg tcgcttttga cctgggcctc
     2221 cgcaaggtga aggttttcgt caagggaccc ggctcaggcc gtgagtcagc tatccgtgcc
     2281 atatcaaaca gcggtgtgga ggtgactgaa atcatagaca ttacgccaat acctcacaac
     2341 ggatgccgtc cgcctgcacg caggagggtt tagaagggtc atattaaaga ttttcaaaac
     2401 gataaatatc tcattcaatg gcaagataca caggacccag atcgcgaatc gccaggaaat
     2461 tcggagagcc tattttcggc cctgacaaat accttgacag gaagaatttt cctcccggcc
     2521 agcacggtct gaccaaaaag agaaaaaaga catcggaata cggtatacag ttgcgtgaaa
     2581 agcagaaggt taaatatacc tatggtctgc ttgagcgtca gttccgcaat acctttgaga
     2641 aggcagtggc tgcctccggt gtgaccggcc tggttctcct gcagatgctg gaatcacgcc
     2701 ttgacaatgt ggtataccgc cttggcatag ctcccacaag accggcagca cgccagctgg
     2761 tgacacaccg tcatatcaca atcaatggca acgttgttaa tattccatca atgatgcttc
     2821 gccccggcga tatcattggc gtccgggaaa agtcaaaatc actcgaagtc attaccgaat
     2881 gcctcggcgc aaaccgccgc tcaaaaatga cctggctcga gtgggatgac acgcagatgg
     2941 ccggaaaata catgagtgtc cctgaacgta ccgaaatacc ggaagatatc aaggaacagc
     3001 ttatagttga
//
LOCUS       DAOPBK010000241         2954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_26480_length_2954_cov_5.183857, whole genome shotgun sequence.
ACCESSION   DAOPBK010000241 DAOPBK010000000
VERSION     DAOPBK010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26480_length_2954_cov_5.183857"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PLA17_10680"
     CDS             complement(<1..1068)
                     /locus_tag="PLA17_10680"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HPK85918.1"
                     /translation="MAGSFSRIHYLRYPGRIILPLFFLLLSCQLRAQDTPGRRRVEIL
                     HSDSMKFEQSDGRTRNRLNGNVSLKHNDLYMDCDSAWYYDDTNQVLAYRNIHIWQGDT
                     IHIRGEYLSYDGNTRKAFLTDNVVLNDKEMQLFTDKIDYDVNTQVANYDTWGRILNGD
                     NTLTSRVGIYYSDRKMLHFRDSVRIDNPDYVMTADTMRYDTSREIAWFTGPTEVVGDS
                     IYLYCENGWSDTRNDISQLMKNAVLDNREQRITGDTLWYDEKSGIGEGFGRVTITDTT
                     DNITGGGNYAWYVKRPEQFLLTGTPWFTLFSDDDTLTLRADTLRAIPVEDTAGVSHRL
                     LKAFYGCRIFSREMQGKCDSLS"
     gene            complement(1143..2492)
                     /locus_tag="PLA17_10685"
     CDS             complement(1143..2492)
                     /locus_tag="PLA17_10685"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK85919.1"
                     /translation="MKKNIILSFALAVMAVYVAAGQQVVESVVAVVGNEVIYLSDIEE
                     QVVQLKAERDPTPVEQLRCRIFEDLLIQKLFLDQARIDSIEVSAENVESDLNNRLNDY
                     IRRAGSEQALETYFNKSMAEIRKDLREVLTNQYIVQETQGTIAKDIAVTPEEVKKFYN
                     TIARDSLPLVPRRVQLSIITVEPPDMEASKAEVRQRLLDLRSRIINGESFSTLAMAYS
                     EDMGSAPRGGELGFNTRGELVKPYAEAAWSLKQGAVSKIVESEFGFHIIQLIERKGDM
                     VNSRHILLKPKLSAEQTEWAILRLDSIANMVRSDSISFSMAAMRYSSDKTTRANGGKM
                     VSQDPAERVNWLTLDELPREINLVVRDMRLGEISDPFRTTDTKDNSVYCIVRLDDEIA
                     AHRVNMQDDYNYLSEAALRKKQGQKYEEWIVDKIRRTYIRISDDFRGCSFHYSGWLE"
     gene            complement(2508..>2954)
                     /locus_tag="PLA17_10690"
     CDS             complement(2508..>2954)
                     /locus_tag="PLA17_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85920.1"
                     /translation="LTSNLVRALYIKVPEEMSELDRIRKLYRSQESDDIQELEDICTR
                     FGLRFEDYNDEWIPFTQLLMEVPFESVDQEQWLARNSAVELKDDRFSYFMAIREYRLR
                     NSVAPFEYIRGQIRTIILNNRRNDFLQKLEDGIYNEAMSNNTLKLY"
BASE COUNT          651 a          813 c          696 g          794 t
ORIGIN      
        1 ggaaagggag tcacacttcc cctgcatctc acggctgaag atcctgcatc cataaaatgc
       61 cttgagcagc ctgtggctta ctcctgcagt gtcctcaacg ggaatggccc tgagagtgtc
      121 agccctcagc gtgagcgtgt catcatcaga aaacaatgta aaccagggtg tgcccgtcag
      181 caaaaactgc tccggacgct ttacgtacca ggcataatta cctccgccgg tgatgttgtc
      241 agtggtgtct gtaatggtta ccctcccgaa accttcacct atgccgctct tctcatcata
      301 ccagagggtg tctccggtaa tgcgctgctc acggttgtca agcaccgcat tcttcatcag
      361 ctgtgaaata tcattgcggg tgtcactcca cccattctca caatagagat aaatgctgtc
      421 accgactacc tcggtgggac cggtaaacca ggctatctcc ctgctcgtgt cataacgcat
      481 agtgtcagcc gtcattacat aatcaggatt gtcaatcctg acgctgtcac ggaaatgaag
      541 catcttcctg tcggagtaat agatgcccac cctgctggta agggtgttgt ctccgttgag
      601 gatacgtccc caggtatcat agttggcaac ctgggtgttc acgtcatagt caatcttgtc
      661 tgtaaagagc tgcatctcct tgtcgttgag aaccacgttg tcagtcagga acgccttacg
      721 ggtgttgccg tcgtacgaaa gatactcgcc gcgtatgtgg atggtatcac cctgccagat
      781 atgaatattc ctgtaagcca gcacctggtt ggtgtcatca tagtaccatg cactgtcaca
      841 gtccatgtac aggtcattgt gtttcaggct gacattgccg ttcagcctgt tacgggtgcg
      901 gccgtcagac tgctcaaatt tcattgaatc agagtgcaat atctctaccc tcctgcggcc
      961 gggggtatcc tgtgcccgaa gctggcacga cagaagcagg aagaaaagag gcaaaataat
     1021 ccggcccggg taacggagat agtgtatccg tgaaaagctg cctgccatgc tccccggatt
     1081 tgtaaacggc aatctgtacc gggatgcgtt tccggatatg aatcctgatg ttattttcct
     1141 ctctattcta accatccgga atagtggaag ctgcatccgc ggaagtcatc gctgatacgg
     1201 atgtacgtcc tcctgatctt gtcaacaatc cactcctcgt atttctggcc ctgctttttt
     1261 ctgagagctg cttcggaaag atagttgtag tcgtcctgca tgttgaccct gtgggcggct
     1321 atctcgtcgt caagcctgac aatgcaatag acgctgttat ctttcgtatc tgtggtacgg
     1381 aacggatcgg atatctctcc cagtctcatg tctcttacca ccaggttaat ctcccttggc
     1441 aactcgtcaa gggtgagcca gttgactctt tcagccgggt cctggctaac catttttccg
     1501 ccgttggcgc gagtggtctt gtcggatgaa tacctcatgg cagccatcga aaaggagata
     1561 ctgtcagagc gtaccatgtt ggcgatgctg tcaagcctga ggatggccca ttcggtctgt
     1621 tcggccgaaa gcttcggctt caaaagaata tgccgcgagt tgaccatgtc tccctttctt
     1681 tcaataagct ggatgatgtg aaacccgaac tcactctcta ctatcttcga aacagcgccc
     1741 tgcttcaggc tccaggcagc ctcggcgtat ggcttgacca gctctccgcg ggtgttgaaa
     1801 cccagttcgc ctcctcgcgg agctgatccc atatcctcgg aatatgccat tgccagtgtg
     1861 ctgaagcttt caccgttgat gatacggctc ctcaggtcaa ggagcctctg acgcacctct
     1921 gccttgctgg cttccatatc aggcggctca accgtaatga tgctcagctg taccctcctt
     1981 ggtaccagcg gaagcgagtc acgtgctatg gtgttgtaaa attttttcac ctcctcggga
     2041 gtgactgcta tatccttggc gatggtaccc tgggtctcct gaacgatgta ctggttagta
     2101 agcacctccc gcaaatcctt gcggatctct gccatgctct tgttgaaata ggtttccagg
     2161 gcctgctctg aaccggcacg ccttatatag tcgttaagcc tgttgttcag gtctgactca
     2221 acgttctccg ccgaaacctc tatggagtca atcctggcct ggtcaagaaa aagcttctgt
     2281 atcagcagat cctcaaagat acggcacctg agctgctcca cgggcgtggg atcacgctca
     2341 gccttgagct gaaccacctg ctcctcgatg tcagaaaggt agattacctc attgcccaca
     2401 acagccacaa ccgattcaac cacctgttgc ccggctgcaa catagaccgc cattactgcc
     2461 agcgcaaatg ataaaatgat attcttcttc atccttttca atcccttcta atagagtttc
     2521 aatgtattgt tgctcatagc ctcattgtag atgccgtctt cgagcttctg caggaaatcg
     2581 ttcctcctgt tgttgaggat tattgttctg atctgtcccc taatatattc aaacggtgcc
     2641 acgctgttcc tcagcctgta ctcccttatt gccatgaaat aggaaaacct gtcatccttc
     2701 agctccacgg cgctgttcct tgccagccac tgctcctggt caactgactc gaacggcacc
     2761 tccataagga gctgggtgaa gggaatccat tcatcattgt aatcttcaaa ccgcagtccg
     2821 aaccgggtgc agatgtcttc aagctcttgg atatcatctg attcctgtga cctgtaaagt
     2881 ttcctgatac ggtcaagctc tgacatttct tccggcactt tgatgtaaag cgccctcacg
     2941 aggttcgatg tcag
//
LOCUS       DAOPBK010000242         2940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_26640_length_2940_cov_5.145234, whole genome shotgun sequence.
ACCESSION   DAOPBK010000242 DAOPBK010000000
VERSION     DAOPBK010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26640_length_2940_cov_5.145234"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="PLA17_10695"
     CDS             <1..1679
                     /locus_tag="PLA17_10695"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPK85921.1"
                     /translation="IHHHYSNDKFVPGFSADYFALLVSGSDADRLPLKEGESADDLTS
                     MLTPVLFDPALYARRVDQTEGVDMVTASANNFYEGVTQAEVEQYYASLADPADPRPVS
                     WGLNSKVMKVDGKVTEIPWKSGGLYGQAIDKIVYWLEKARDVALSETQKNELDLLISY
                     YRSGDLKTWDEYNVVWAGNTELSVDYINGFIETYGDPMGMKATWEAVVDYKDSEASKR
                     TALITENAQWFEDHSPVDPQYRKEKVTGVAASVINVAMLGGDCYPASPLGINLPNSNW
                     IRAEAGSKSVTLANITDAKDIVSRGSGFLEEFAFDQAEIDRIVTYGSISDALHTDMHE
                     CIGHASGKLAHGTDPNALKNYASPLEEMRADLFALYFMMDPKMTEIGLMPSDEPARAL
                     YDGYIRNGFLTQIVRIQPGKDIEQAHMRCRSAIAHWVYEKGRPENVIEVVKRDSKTFV
                     RINDYQKLRGLFGEMLREVQRIKSEGDFEAGRSMIENYGVKIDQELHGEILARYAGLN
                     LAPYSGFVNPVMTPVTDRKGRITDVKIEYCSDFLGQMMEYGKKYSFLPVW"
     assembly_gap    1736..1835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1836..2263)
                     /locus_tag="PLA17_10700"
     CDS             complement(<1836..2263)
                     /locus_tag="PLA17_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPK85922.1"
                     /translation="MSKIIHHPVDNVTMRLIKSEDLNHHGTLFAGRTAEWFVESGFIA
                     STSLLDPKNVVCLKVHGMHFTSPANPGEVLKFDSKIVYTGRSSLVAYITICKATCPKI
                     LVEGFATFIHVDEETRPVPHNIVTEPLTEADRLLYEQAANL"
     gene            complement(2256..>2940)
                     /locus_tag="PLA17_10705"
     CDS             complement(2256..>2940)
                     /locus_tag="PLA17_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPK85923.1"
                     /translation="AFGAYSLGANNIANVMGVFVPSIPLEDADFGIFVLSGAQQLFFL
                     GGIAIAAGILTYSKKVIETVGTNLMELSSEAAFVVVLAQALVLFIFSSSSLSDFMVRI
                     GLPPIPLVPVSSTQVVIGAILGIGLYKGVRNINLKVLGSIASGWITTPVAAGILSFLL
                     LFVVKNLFSIDVGHSVNPPASAGKGMSMAAIIRYSIPVLLFITAIYLVWLILSEKKKT
                     ERINKKYYE"
BASE COUNT          753 a          645 c          768 g          674 t
ORIGIN      
        1 gcatacacca tcactattca aatgataagt ttgtgcccgg cttttcagca gattattttg
       61 cccttctggt cagcggttct gatgcagacc gcctgccttt gaaagaagga gagagcgcag
      121 atgaccttac atccatgctc acaccggtgc tgtttgaccc tgcactgtat gcaagaaggg
      181 ttgaccagac agaaggtgtt gacatggtaa ccgcatcagc caacaatttt tatgagggag
      241 taacacaggc cgaggtggag cagtattatg cctcgctcgc tgatcctgct gacccgaggc
      301 cggtttcttg gggactgaat agcaaggtga tgaaggttga cggcaaagtg accgagattc
      361 catggaagag tggcggactg tacggtcagg ctattgacaa aatagtctac tggcttgaaa
      421 aagcgcgtga tgttgcgctg agcgaaacac agaagaacga gcttgacctg ctcatcagct
      481 attacaggag cggagatctg aagacatggg atgagtacaa tgtggtatgg gctggaaata
      541 ccgagctgtc agttgattat atcaacggat tcattgaaac atatggtgat ccgatgggta
      601 tgaaagccac atgggaggct gtggttgatt acaaggactc tgaagcctcc aaaaggacag
      661 ctctgatcac ggagaacgcc cagtggttcg aggatcattc acccgtcgac ccgcagtacc
      721 gcaaggagaa agtgacaggt gtcgctgcaa gtgtcatcaa cgtggcaatg cttggaggcg
      781 actgttatcc ggcctcgccg ctggggatca accttcccaa ttcaaactgg ataagggcag
      841 aagcagggtc caagtcagta accctggcaa atattaccga tgccaaggat atagtgtcac
      901 ggggaagtgg cttcctggaa gagtttgcct ttgaccaggc cgagattgac cggatagtca
      961 catacggttc aatttctgat gcattgcata ccgacatgca tgagtgcatt ggccacgcaa
     1021 gcggcaagct ggctcacggc actgatccca atgccctgaa aaactacgct tcaccactgg
     1081 aagagatgag ggctgacctg tttgcccttt actttatgat ggatccgaag atgacagaga
     1141 tagggcttat gccttctgac gaacctgcca gggcactcta tgacggttat atacgcaatg
     1201 gtttcctgac ccagatagtg agaatacagc caggcaagga catagaacag gctcatatga
     1261 ggtgccggtc ggcgatagcc cactgggtat atgagaaggg cagacctgag aacgtgattg
     1321 aggtggtgaa gcgtgacagc aagacgtttg tccggataaa tgattatcag aaactgcgcg
     1381 gacttttcgg ggagatgctc agggaggtgc agagaatcaa gtcagagggt gatttcgaag
     1441 ctggcaggag catgattgaa aactacggtg taaagataga tcaggaactt catggtgaaa
     1501 tactggcaag gtatgccgga ctgaatctgg ctccctacag cggattcgtc aatccggtca
     1561 tgacacctgt aacagaccgc aagggcagga taacagatgt aaaaattgag tattgctctg
     1621 acttcctggg acagatgatg gaatacggga agaaatactc cttccttccg gtctggtgaa
     1681 ttgtcagaat cagcctggct cccggggagc atctctgatc aggacttctt cagatnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagatt cgctgcctgc tcgtacagta
     1861 atctgtcagc ctcggtcaga ggttcggtga caatgttgtg gggcacgggt cgggtctcct
     1921 catcaacatg aatgaaggtg gcgaaacctt caaccaatat cttgggacaa gttgccttgc
     1981 atatagttat ataagccacc aggcttgatc tgcctgtata gacaattttg gaatcaaact
     2041 tcagcacctc tccgggattg gccgggctgg tgaaatgcat gccatggacc ttcagacaga
     2101 ccacattttt gggatcgagc agactagtgg aggcaatgaa gcctgattca acgaaccact
     2161 cagccgtacg ccctgcgaaa agagtgccat gatggttcag atcctcactc ttaatgaggc
     2221 gcatggtgac attatcaacc ggatgatgaa tgatcttact catagtattt tttatttatc
     2281 cgttcagttt ttttcttttc tgacagtata agccagacca ggtatattgc agtgatgaaa
     2341 agcagcaccg gtatgctgta ccggataatt gccgccatgc tcatcccttt accggctgat
     2401 gccgggggat tgaccgagtg ccccacgtca atgctgaaaa ggttttttac gacgaagagc
     2461 aaaaggaaag atagtattcc tgcagccacg ggtgtggtta tccatcctga agcaatgctt
     2521 ccaagcactt tcaggttaat gtttctgacc cctttgtaaa ggcctattcc aagtattgcg
     2581 cctattacca cctgcgtgct tgagaccggc accagcggga tgggtggaag acctatcctt
     2641 accatgaaat ctgacaatga agatgatgag aagataaaca gtaccagtgc ctgtgcgagc
     2701 actaccacga aagcggcctc ggatgaaagc tccatgaggt tggttcctac tgtctctatc
     2761 actttctttg aatatgtcag tattccggct gctatggcga tgccaccgag aaagaagagc
     2821 tgctgtgccc cgctgagaac gaagatgccg aagtcagcat cctcgagagg gatgcttggc
     2881 acgaatacac ccataacgtt ggctatgttg tttgccccaa ggctgtaggc gccgaatgcg
//
LOCUS       DAOPBK010000243         2932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_26733_length_2932_cov_3.874174, whole genome shotgun sequence.
ACCESSION   DAOPBK010000243 DAOPBK010000000
VERSION     DAOPBK010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26733_length_2932_cov_3.874174"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..669)
                     /locus_tag="PLA17_10710"
                     /pseudo
     CDS             complement(1..669)
                     /locus_tag="PLA17_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(832..>2932)
                     /gene="gcvP"
                     /locus_tag="PLA17_10715"
     CDS             complement(832..>2932)
                     /gene="gcvP"
                     /locus_tag="PLA17_10715"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPK85924.1"
                     /translation="NQRFGLPMSYGGPHAGFFATRDEMKRNMPGRLIGVSIDAHGNLA
                     LRMALQTREQHIKRERATSNICTAQALLATMSGMYAQYHGPEGLKRIARHIFSMACTL
                     AGALNKLGYTIKHEQFFDTVCVQAIPGVTSEMIEKIALRREINLWYPCANCVLISTDE
                     VTTLKEINEIVAIFAEAAGKKAEEVTSLCDCFCIDEKFQRKSAFLEEHNFNSYHSETD
                     LMRYIKMLERKDFSLANGMIPLGSCTMKLNSAVSMYAMSWPEFGNIHPFVPADQAEGY
                     HKMMEELGEALKEITGFDAVSFQPNSGAAGEYSGLMVIRQYHISRNEGYRNVALIPSS
                     AHGTNPASAAMAGMKVVVVECDDNGNISVEDLRKKAVEHRENLSAAMITYPSTHGVFE
                     EAITGMIKIVHENGGLVYMDGANMNAQVGLTSPGHIGADVCHLNLHKTFAIPHGGGGP
                     GMGPILCNTKLAEFLPAHPLVKTGGSHGITAVSAAPFGSAHVLTISHAYIMMLGADGL
                     TKSTVCAILNANYISAKLSGMYKVLYTGANGRCAHEMILDCRGFKEMGVDETDIAKRL
                     MDYGFHAPTLSFPVHGTLMVEPTESEPKVELDRFIDAMRSIWNEIEEIRNGKADREDN
                     VLKNAPHTAAAVISDDWKHAYAREKAAFPLAWVVENKFWPTVGRVDNGYGDRNLVCTC
                     APIDSHRHKTFKSAGEK"
BASE COUNT          687 a          791 c          664 g          790 t
ORIGIN      
        1 agctccattt ccaacaagaa aagcattaac tccggtatta gcatcggatg cccacccggg
       61 cataccccct cctcctgagg gtgttgcatt tgatccatta aagcaaattt ctgaaaaata
      121 ttgtaaaggt tcaagataaa ctcttacttt agtttctctg attacaaggg ttttacttga
      181 atgggcatta gttgggcaat ctctattatc aaaattttcg taacttgcgg gcattctgat
      241 ccagaattca gcaaatcctg ttttttcgtc ataccttata gcagtagttt tccatccact
      301 aacttgtgat tcagcatatt gcaaattacc tccatgattg aagtactctc ttaaaaatac
      361 ttcgatatca ccagggtcgg ttatggttat agcatcataa gaacgggctc ttgcataacc
      421 gcttgggtca atatacttga ggggattatt aatacagtaa ctataattat tcagatcatg
      481 cgaactataa ggattttgga ttatcggatc tacattcaga aatctactga tcaacggatc
      541 ataaactctc ccattcatgt ttattaatcc aaacatatca agatgctcct ggccggtgta
      601 ccccaaagat gtcaacttca attccgtaat actgtcatat gaccagtttg taggattacg
      661 ctgtctcccc catgaatcat aagaatgatg ttctgcttag ctaccagtac tgttaatcag
      721 gcctataatg gaaccaaaat aatccttctc tatataatac aatctttcaa ctgttccctt
      781 gttaactgtt atcgcttcaa tgccatactg cagcccccgg cggggtgtac actatttctc
      841 cccggctgat ttaaatgtct tgtgcctgtg ggagtcaatc ggtgcacagg tgcagaccag
      901 gttgcggtca ccgtaaccat tgtcaaccct gccaaccgtt ggccagaact tgttctccac
      961 cacccatgcc agcgggaacg cagccttctc ccgggcataa gcatgcttcc agtcgtcaga
     1021 gatgactgct gcagccgtat gcggtgcatt cttaagcaca ttgtcctcac ggtcagcctt
     1081 gccattccgt atctcctcaa tctcattcca gattgaacgc atggcatcga taaacctgtc
     1141 aagctcaacc ttcggctctg actcggtagg ctcaaccatc agtgttccgt gaaccgggaa
     1201 ggagagcgta ggtgcatgga acccatagtc catcagcctc ttggctatat ctgtctcatc
     1261 cactcccatc tctttaaagc cacggcagtc aagtatcatc tcatgggcac agcggccgtt
     1321 ggcgccggtg taaagcacct tgtacatgcc acttagctta gcagagatat agttggcatt
     1381 aagtattgca cagacagttg actttgtcag tccgtcggcc cccagcatca tgatataggc
     1441 atggcttatg gtaagcacat gagcgcttcc gaaaggcgct gcggaaacag cggtgatccc
     1501 atgactgccg cccgtcttta ccagcgggtg tgccggcagg aactcagcca gcttcgtgtt
     1561 gcacaggatg gggcccattc ccggcccgcc accaccgtga ggtatggcaa aggtcttgtg
     1621 caggttgaga tgacagacat cagccccgat gtgacccggg ctggtaaggc ctacctgcgc
     1681 attcatgttg gcgccgtcca tatagacaag cccgccattc tcatgaacaa tttttatcat
     1741 cccggtgata gcctcctcaa acacaccatg cgttgagggg tatgtaatca ttgcggcgct
     1801 caggttttcg cggtgctcaa ccgctttctt cctcaggtcc tcaacgctga tattcccgtt
     1861 gtcatcgcac tccaccacaa ccaccttcat gccggccatg gcagcactgg caggattggt
     1921 gccatgtgca gatgatggta tcagtgccac gttgcggtac ccttcattcc ggcttatatg
     1981 gtattgcctg atcaccatca ggccgctgta ttcacccgcg gctcccgagt tgggctggaa
     2041 ggagacagca tcaaagcctg ttatctcctt gagagcctcg ccaagctctt ccatcatctt
     2101 gtggtaaccc tccgcctggt cggcgggaac aaacgggtgt atgttgccaa actccggcca
     2161 gctcattgca tacattgata ctgcagaatt gagcttcatg gtgcatgaac ccagcgggat
     2221 catcccgttg gccagcgaaa agtctttacg ctccagcatc ttgatataac gcataaggtc
     2281 agtctcactg tggtagctgt tgaaattatg ctcctccagg aaggccgact tccgctggaa
     2341 tttttcatcg atgcagaagc agtcgcacag cgaagtaacc tcttctgcct tctttccggc
     2401 agcctcggcg aaaattgcaa ctatctcatt tatctctttc agtgttgtga cctcatctgt
     2461 gctgatcaac acgcagttgg cacagggata ccagaggttg atttccctcc tgagcgctat
     2521 cttctcaatc atttccgagg tgactcccgg tatagcctgt acacataccg tgtcaaagaa
     2581 ctgttcgtgc tttatggtat atcccagttt attcagcgca ccggccaggg tacatgccat
     2641 tgagaagata tggcgggcta tgcgcttcag cccctcaggg ccatggtact gggcatacat
     2701 tcctgacatg gtggcaagca gtgcctgtgc agtacatatg ttagaggtgg cgcgctcacg
     2761 cttgatatgc tgctcccgtg tctgcagcgc cattcgcagc gccaggtttc cgtgtgcgtc
     2821 aattgagaca cctatcagcc gtcccggcat gttacgcttc atctcatccc tggtggcaaa
     2881 gaatccggca tgcgggccgc cgtagctcat cgggaggcca aacctctggt tg
//
LOCUS       DAOPBK010000244         2895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_27145_length_2895_cov_4.829930, whole genome shotgun sequence.
ACCESSION   DAOPBK010000244 DAOPBK010000000
VERSION     DAOPBK010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27145_length_2895_cov_4.829930"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1142)
                     /locus_tag="PLA17_10720"
     CDS             complement(159..1142)
                     /locus_tag="PLA17_10720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPK85925.1"
                     /translation="MTDVTCAVIRNEERLVLAVRRGPGMNNAGKWEFPGGKTRPGEDH
                     EECIIREIDEELGMEIVICGRLEAVEHDYGNIRIRLIPFICDTLASKPLLSEHDQYRW
                     MEPDELMTLDMTAADIPVALQYASGHRCAANSETEEETAGITTEVGEELSCMIGRITS
                     VEEIIMVAQSAVNDPNLLSQLVGLCSSTEKRVGFMASWALAKVVDVDLKAMVPFLSLV
                     IDVLPSAPNESVQRSLMRVLTRSRTSDIPQSHHAKLIDYCMTLMRVPNVPVAPKAYAM
                     EILASLCELYPEMVNEVAGAVQLAINDASWGMQAAGRKVIQRLGRGNSLKD"
     gene            complement(1139..1603)
                     /locus_tag="PLA17_10725"
     CDS             complement(1139..1603)
                     /locus_tag="PLA17_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPK85926.1"
                     /translation="MIPLKLPPCSLRIKAGPGNRLVYDSLRRKYVALTPEEWVRQHFV
                     NYLTGFLGYPPGLIKVETSFRLHGMLRRADILVHDRDGEPVLIVECKAPQVKITQEVF
                     DQIINYNFSYGVPYLVVTNGITHFAARTDIAKRSFELLDSIPDYETIIRREP"
     gene            1666..2436
                     /locus_tag="PLA17_10730"
     CDS             1666..2436
                     /locus_tag="PLA17_10730"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPK85927.1"
                     /translation="MKTKEDIVSNWLPRYTGKELSSFGEFILLTNFTHYVETFARWND
                     VPVEGKDKNWPSATAEGITIINFGMGSPNAATVMDLLSSIQPKAVLFLGKCGGLKKKN
                     KLGDLILPIAAIRSDGTSNDYLPLEIPALPAFKLQSAVSSAIVQNNLEYWTGTVYTTN
                     RRVWEYDDRFKKYLVKTRAMAIDMETATIFTVGFANEIPTGALLLVSDQPMISSGIKT
                     DESDQKVTSQFADNHLRIGIEALTEIKNNGISVKHLRF"
     gene            2436..>2895
                     /locus_tag="PLA17_10735"
     CDS             2436..>2895
                     /locus_tag="PLA17_10735"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPK85928.1"
                     /translation="MAVDYKDIIRDLRNKIYKPVYFLAGKEPYYIDHITDFIEENVLD
                     ESEKAFNQTVLYGLDVSVTQVIEICRRFPMMANHQVVVVREAQKLKKIEELLLYVENP
                     LNSTILVIAYKYELPDKRKSLYKALSKRGWYFESDKVPEYQIPGWIDRYLT"
BASE COUNT          715 a          775 c          699 g          706 t
ORIGIN      
        1 tagatgcaga atttgaatac aggcgtttac acaaagtatt ctatagactc tttttaaata
       61 cggttctacc gatattttaa tggatagagg tagaagcctc catgaaaaaa gtatatgagc
      121 ctgtgaaaat cttaatgtaa acgagtagct gcgggctttc agtctttcag gctgttacct
      181 ctgcccagtc tctgaatgac cttccgtcct gccgcctgca tgccccatga cgcatcattg
      241 atggccagct gtactgctcc ggcaacctcg ttgaccattt cggggtagag ctcacacaat
      301 gacgcaagta tctccatcgc gtatgccttt ggcgccaccg gcacgtttgg cacgcgcatg
      361 agcgtcatgc agtagtcaat cagtttcgca tggtgcgatt gggggatgtc actcgtcctg
      421 ctcctggtca gtaccctcat caacgatctc tgcacgctct cgttgggtgc tgagggcagt
      481 acatcaatga ccagcgaaag gaacgggacc atcgctttca gatcaacgtc aaccacctta
      541 gcgagagccc atgaggccat aaacccaacc ctcttttcag tactggaaca gaggccaacc
      601 agttgtgaca gcaggtttgg gtcattcacc gccgattggg ccaccattat tatctcctcc
      661 acgcttgtga tcctccctat catacaggag agctcttccc ccacttcggt ggtgattcct
      721 gccgtctcct cctctgtttc gctgtttgcc gcacacctgt gacctgaagc atactgaagg
      781 gcaacaggta tgtccgcagc tgtcatgtca agtgtcatca gctcgtcagg ctccatccac
      841 ctgtactgat catgctcgct gagaagaggc tttgatgcca gggtatcgca gatgaaaggg
      901 atgagcctga tccggatatt gccgtaatca tgttcaacgg cttccagcct gccgcagatg
      961 acaatttcca tccccagctc ctcatctatc tccctgatga tacactcttc gtgatcctct
     1021 cccggcctgg tcttgcctcc ggggaactcc cacttgccgg cattgttcat gcccggtccg
     1081 cgccgcacag ccagaacaag cctctcctca ttgcgtatca ccgcgcatgt aacatctgtc
     1141 atggctccct tctgatgatg gtctcatagt cgggtatact gtccagcagc tcgaagcttc
     1201 gctttgcaat atctgtcctg gcggcgaagt gggtgattcc gtttgtgaca acgaggtatg
     1261 ggacaccgta gctgaagttg tagttgataa tctggtcaaa gacctcctgt gtgatcttca
     1321 cctgcggggc cttgcactcc actatgagca ccggttctcc atccctgtcg tgtaccagga
     1381 tgtcagcccg ccgcaacatc ccgtggagcc tgaatgatgt ctcaaccttt atcagtccgg
     1441 gcggatagcc caggaaaccg gtcaggtaat tgacgaaatg ctgcctgacc cactcctccg
     1501 gagtaagtgc cacgtacttc cttcgaagtg agtcataaac aagcctgttg cccggacctg
     1561 ctttaatccg aagtgaacat ggaggcagtt tcagaggtat caacttttat tgtatttttg
     1621 gttaccctta tgcagcacaa atataaacaa acagcatctg agaacatgaa gaccaaggaa
     1681 gatatagtaa gcaactggct tccccgttac acgggaaaag agctcagctc attcggcgag
     1741 tttattctgc tgacaaattt tacacattat gtggaaacct ttgccagatg gaacgatgtg
     1801 ccggttgaag ggaaggacaa gaactggccc agcgccaccg cggaaggtat aacaatcatc
     1861 aacttcggca tgggcagtcc caacgcagcc acggtgatgg atctcctcag ctccatccag
     1921 ccgaaggctg tgctcttcct tggcaagtgc ggagggctga agaagaagaa caaactgggt
     1981 gaccttattc ttccgatagc tgcaataaga agcgacggta catccaatga ttaccttccg
     2041 ctggagatcc ctgcgctgcc tgcgttcaag ctgcagagcg ccgtctccag cgccatcgtg
     2101 cagaacaacc tcgagtactg gaccggtacc gtttatacca ccaaccggag ggtctgggag
     2161 tatgatgacc gcttcaaaaa atacctggtt aaaacgaggg caatggccat tgacatggag
     2221 acagcaacaa tctttaccgt aggttttgcc aatgagattc ccaccggagc gctcctcctc
     2281 gtctctgacc agcctatgat ctccagcggt atcaagacag acgagagcga ccagaaggtc
     2341 acaagtcagt tcgcagacaa tcatcttcgc attggcatag aggccctcac ggaaataaag
     2401 aataacggta tctcggttaa acatctacgc ttctgatggc tgtcgattac aaggatataa
     2461 taagggatct gcgcaacaag atctataagc ccgtttattt cctggccggg aaggagccat
     2521 attatattga ccatattact gatttcattg aggaaaatgt gcttgacgag tcggagaagg
     2581 cattcaacca gaccgtgctc tatggcctcg atgtgtcagt gacacaggta attgagatct
     2641 gccgccgctt cccgatgatg gcaaaccacc aggtggttgt cgtacgggaa gcacagaaac
     2701 tcaagaagat tgaagagctt ctgctttatg tcgagaaccc cctcaattca accattctcg
     2761 tcattgcgta caagtacgaa ctccctgaca agaggaaatc tctttacaaa gcgctttcca
     2821 aaaggggatg gtactttgaa tcggacaagg tgccggagta tcagattccc gggtggattg
     2881 acaggtacct gaccg
//
LOCUS       DAOPBK010000245         2888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_27220_length_2888_cov_4.600424, whole genome shotgun sequence.
ACCESSION   DAOPBK010000245 DAOPBK010000000
VERSION     DAOPBK010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27220_length_2888_cov_4.600424"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="PLA17_10740"
     CDS             <1..1450
                     /locus_tag="PLA17_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85929.1"
                     /translation="GGLFKVFINSSLKGERPCLFIPVVFQGAPRVKEPAEEIRQPAVN
                     DPVSPADVSPEQRTEGAMSNDTVGRQSGEETDSVPATAVIPAGELESEPQETITAEPE
                     SAGARNTLQKLRSVFLLSGDNPWLARHNYFGKSVTLVNALIIAIVVSFATMLILLVVI
                     LFNRRRMEKEARLRQYLLEQYQTLIIDYLFGSSGPEPFRKIASDYYRRQVLIDQMIDV
                     SVNLKGDSREKLKKLYLDLELDEDSLDRAYSRRWHKKIKGFRELAFMGITEGNDEMYR
                     SLNSRNEILRMEAQIALVRLSEKDHFEFLSQLKRPFSLWEQITLHDLILQHDISVPDF
                     QRWLNSENPTVVMFALRMIREFKQIEAEPEVRKVLLHREPRVSRLAVEVAGDLGMKTT
                     LETMKRMYKFQEYNSCLEIVRSMGKMPETSMLGFLKLVLDKEDDVQLQIEAVKAIENM
                     GEIGVQALVKIMKSEYKNYNIIVRHVLDRRIN"
     gene            1456..2871
                     /locus_tag="PLA17_10745"
     CDS             1456..2871
                     /locus_tag="PLA17_10745"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK85930.1"
                     /translation="MGFIFAHLIFWLTLVLFTTYLLLGIYSALALRKYLRKNSYVNYN
                     SLVLSPLSPRITIIAPAFNEGKTIIDNVRTLLSLYYNNFEVIIVNDGSTDDTFSKIRD
                     AYELVRVNYYFDYRIPCERIRGVYRSKDPAYNRLTVIDKNNGGKADSLNAGINISRSD
                     LIVTIDADSIIEPDSILKLVKPFLEEKDKKVIGTGGVIRIVNSCDVERGHIRQINLPK
                     KFLPRVQVLDYTRAFLLGRMAWSHLDGLMLISGAMGMFDRETVIRAGGYSIKTVGEDM
                     ELVLRMRRYMAERKQKYEVTYIPDPLCWTEVPADLRSMRKQRTRWTRGLIESLWTHRK
                     MMLNVKYGRLGMLGYPYWFMFEWAAPLIAFLGIVYSIYLAITGALYWPFFLLLFLFVY
                     SFAVSLTTWAVLFEEITFHKYRRKRDVLRLIGTAMLEPFFYPVHTYFAVRGNLESLRG
                     KKGWGKAERSGFQNKKKPATA"
BASE COUNT          761 a          707 c          761 g          659 t
ORIGIN      
        1 gggtggtctg ttcaaggtat tcataaacag ctcactgaaa ggagagaggc catgcctttt
       61 cattccggtt gtattccagg gagcaccccg ggtaaaggaa cctgcagagg agataaggca
      121 gcctgcagtc aatgacccgg tctcgccggc agatgtctca cccgaacagc gcacggaagg
      181 tgcaatgtca aacgatacag tcggccggca atccggtgaa gaaactgatt cggtacctgc
      241 caccgcagtg atacccgcag gagagctgga gtctgaaccg caggagacca ttacagctga
      301 accggaatca gccggagcac ggaatacatt gcagaaactg aggagtgtct tccttctgtc
      361 gggagacaac ccatggctgg ccaggcataa ttacttcggg aaatcggtga cgctggtgaa
      421 tgcattgata attgccatag tagtctcctt cgcaacaatg ctgatcctcc tggtggtaat
      481 tctcttcaac aggagaagga tggagaagga ggccagactg aggcagtacc ttctggaaca
      541 gtaccagaca ctcatcatag attacctttt cggcagcagt ggtcctgaac ctttcagaaa
      601 gattgcatct gactattacc gcaggcaggt gctcattgac cagatgattg acgtgagcgt
      661 gaacctgaag ggcgattccc gcgagaagct taagaagctt tacctggatc tggaacttga
      721 cgaggattcg ctggacaggg cctacagccg gcgctggcac aagaagatca agggtttccg
      781 cgagcttgcc ttcatgggta tcacagaggg gaatgatgaa atgtacaggt cactgaactc
      841 caggaatgag atactccgga tggaggccca gatagcattg gtgcgcctga gcgagaagga
      901 ccacttcgag ttcctttcac aactgaagag accattctca ctgtgggaac agatcaccct
      961 tcatgatctg atactgcagc atgatatttc tgtacctgac ttccagagat ggctcaactc
     1021 ggagaacccg acagtggtga tgttcgccct caggatgata agggaattca agcagattga
     1081 ggctgagccg gaggtcagaa aggttctcct tcaccgtgaa cccagggtca gcaggctggc
     1141 agttgaggtg gccggcgatc ttggcatgaa aaccaccctt gagaccatga aacgcatgta
     1201 taagttccag gagtacaaca gctgcctgga gattgtcagg tcaatgggca agatgcccga
     1261 gacttccatg cttggattcc tcaagctggt acttgacaag gaggatgacg tgcagctgca
     1321 gatagaggct gtgaaggcaa tagaaaacat gggcgagatt ggagtacagg cccttgtcaa
     1381 gattatgaaa tcagagtaca agaactataa tataatagtc aggcatgtgc ttgaccgcag
     1441 gataaactga ttagcatggg attcattttc gcacatctga tattctggct gaccctggta
     1501 ctctttacca cgtacctcct cctcggtata tattcagccc tggctctcag gaagtacctt
     1561 agaaagaaca gttacgtcaa ttataactcc ctggttctct cacccctgag cccccggatc
     1621 accatcattg ccccggcatt caatgagggc aagactatca ttgataacgt ccgcacactg
     1681 ctttcactct actacaataa ctttgaggta ataatcgtca acgacggatc gaccgatgat
     1741 accttctcaa agatcaggga tgcctatgaa ctggtcaggg tcaattacta ttttgactac
     1801 cgcataccct gcgaaaggat caggggtgtc taccgctcaa aggacccggc ctacaacagg
     1861 cttactgtca tcgataagaa taacggaggc aaggccgact cactaaatgc cggaatcaat
     1921 ataagccgca gtgacctgat agtaaccatt gatgctgact ccatcattga acccgattcg
     1981 atactgaagc tcgtcaaacc attcctcgag gagaaggaca agaaggtcat cggcacgggt
     2041 ggtgtaattc gcattgtcaa ctcatgtgat gtggagcgtg gtcacattcg tcagatcaac
     2101 ctgccgaaga agttcctccc aagggtgcag gtgcttgact atacgcgtgc attcctcctc
     2161 ggccgtatgg catggagcca tcttgacgga ctgatgctga tatcaggagc catgggcatg
     2221 tttgaccgtg agacggtaat cagggccggc ggctacagca tcaagaccgt tggcgaagat
     2281 atggagctgg tactgaggat gaggcgctat atggccgaac gcaaacagaa atacgaggtt
     2341 acctatatac ctgacccgtt atgctggacg gaggtacccg ctgacctcag gtcaatgcgc
     2401 aaacagagga caaggtggac gagaggactg attgaatctc tctggacaca ccggaagatg
     2461 atgctgaacg tgaaatacgg gaggctgggg atgctcggct atccctactg gttcatgttt
     2521 gaatgggcag cgcccctgat tgcattcctc ggaatagtct attcaatata tcttgcaatt
     2581 accggtgcac tgtactggcc cttcttcctg ctgctcttcc tctttgtcta cagcttcgcc
     2641 gtaagcctta ccacatgggc tgttctcttt gaggagatta ccttccacaa gtatcgcagg
     2701 aaacgtgatg tgctccgcct gataggcaca gccatgcttg aacccttctt ctatccggtc
     2761 catacctatt ttgctgtcag gggcaacctt gagtcgctcc gaggcaagaa aggctggggc
     2821 aaagccgaaa gaagcggatt ccagaacaag aaaaagccgg ccactgcctg atttattgct
     2881 gtctgatt
//
LOCUS       DAOPBK010000246         2880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_27336_length_2880_cov_5.845310, whole genome shotgun sequence.
ACCESSION   DAOPBK010000246 DAOPBK010000000
VERSION     DAOPBK010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27336_length_2880_cov_5.845310"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..582)
                     /locus_tag="PLA17_10750"
     CDS             complement(7..582)
                     /locus_tag="PLA17_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85931.1"
                     /translation="MKKAILFVLVLFAGLSLTAQDYQILATKGAVRLFADKEDLTSVI
                     QIIPDGTVVEAISADTLFTLIRLGEVEGYVKSDRLTAALPVVTTTQPEVQPVQEAAPV
                     QPVTDVQARQISTDRFQTLVDKYGTDLASKIYQHKVWKGITSDMARDSWGKPKQINKM
                     YVDNSIDEEWIYSKSYLYFRDGVLIEWGPVK"
     gene            complement(690..1322)
                     /locus_tag="PLA17_10755"
     CDS             complement(690..1322)
                     /locus_tag="PLA17_10755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPK85932.1"
                     /translation="MEKELDKYIREHSTPEDKVLEELYRQTHLYVVNPNMVSGHIQGK
                     FLEMLSYMISPVSVLEIGTYTGYSAICLARGLKSGGQLHTIEINDELNGMSTRYFALA
                     GVADRVTLHTGRAQDVIPKLDRTFDLVFIDGDKREYCEYYDIVFDKVRTGGFIIADNV
                     LWGGRIEGEEAMKDPQTRGLVLFNEKVRNDHRVEKIVLPLRDGLMIIRKK"
     gene            complement(1306..2544)
                     /locus_tag="PLA17_10760"
     CDS             complement(1306..2544)
                     /locus_tag="PLA17_10760"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK85933.1"
                     /translation="MDITLHTLPNGIRLVHLPTTSAVAHCGIIINAGSRDELPEEHGV
                     AHFIEHMLFKGTTHRKSYHILSRLEDVGGELNAYTTKEETAIHASFLREHYPKAVELI
                     SDLLFSSTFPPAEIEKEKEVIIEEINSYLDSPAEYIFDEFEEMIFPEQPIGRSILGMP
                     GTVRGISADMIRRFIARNYSTSQMVFCSVGAITPKKIITLFEKYFGHVASGNDTRRKR
                     SEYLYNPVSKEKKTDTWQNHCIIGNIAYDLHDKRRMGLFMLNNILGGQGMNSRLNLSL
                     REKRGYAYNVESMYNPYTDTGLVTIYFGTDNRNLGKSIALIHSELDRLRHQPLGILQL
                     SRAKNQIKGYLARAYENHESLMLSLGKSLLIFDRIDSIDETFRKIEAVTGSELMEIAS
                     EIFDKSSLSTLIYRQNGERA"
     gene            2698..2770
                     /locus_tag="PLA17_10765"
     tRNA            2698..2770
                     /locus_tag="PLA17_10765"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2731..2733,aa:Phe,seq:gaa)
BASE COUNT          666 a          693 c          701 g          820 t
ORIGIN      
        1 aagcttctat tttacagggc cccattcaat aagaaccccg tccctgaaat agagatacga
       61 ctttgaataa atccactctt cgtcaatact gttgtcaaca tacatcttgt taatctgttt
      121 cggcttgccc cagctgtcgc gtgccatatc cgaggtgatg cccttccaaa ccttgtgctg
      181 gtaaatctta ctggcaaggt ctgtcccgta cttgtcaaca agcgtctgaa atcgatctgt
      241 ggatatctgc ctcgcctgaa catcagtgac tggctgtacg ggtgcagcct cctgcaccgg
      301 ctgtacttcg ggctgagtag ttgtaaccac cggtaatgcc gccgtaagcc tgtcagattt
      361 tacataaccc tctacctcgc ccaggcggat aagggtaaac agggtatctg cactgatcgc
      421 ctcaaccaca gtgccgtcag gtattatttg aatcaccgag gtgaggtctt ccttatcggc
      481 aaacagcctt accgcacctt ttgtagcaag tatctgataa tcctgtgccg tcagcgagag
      541 gccggcaaac agcactaata caaaaagtat tgcctttttc atttgctgat ttttaatata
      601 ctatcgctca aaaatcgtac caccattgct tctttccggt attcataagt tagcagcgct
      661 gatgcccctg ccgtgcgcat tgagataaac tacttctttc tgatgatcat aagtccgtca
      721 cggaggggga gtactatttt ttccacccgg tggtcattac ggaccttttc attgaacagg
      781 acaagacccc tggtctgagg gtccttcatt gcctcctcgc cttctatcct gcctccccag
      841 agaacattgt cggcaatgat gaatccccct gtccgtacct tgtcgaagac gatgtcatag
      901 tattcacaat actccctttt gtcaccgtca atgaagacca ggtcgaaagt acgatcaagt
      961 ttcgggatca catcctgtgc cctgccggtg tgcagtgtca ccctgtcagc cacgccggcg
     1021 agggcgaagt agcgggtgct cattccgtta agctcatcat ttatctcaat ggtgtgcaac
     1081 tggccgccgc tcttcagtcc gcgtgccagg cagatggcag agtagccagt ataggtgcct
     1141 atctccagta cactgacagg gcttatcata tatgagagca tctccaggaa cttgccctgg
     1201 atatgtcccg agaccatgtt ggggttgacc acgtacaggt gggtctggcg gtaaagctcc
     1261 tccagcactt tgtcttcagg ggtgctgtgt tccctgatat atttgtcaag ctctttctcc
     1321 attctgcctg tatattaagg tagaaagtga ggatttgtca aatatttcag atgcaatttc
     1381 catcagttca ctgccggtga ctgcctcaat ctttctgaaa gtctcatcta ttgagtcaat
     1441 ccggtcaaag atcagcaggc ttttgccgag actgagcatg aggctctcat ggttctcgta
     1501 agctcttgcc agatagcctt ttatctggtt ctttgcccgt gaaagctgta gtatccctag
     1561 tggctgatgt ctcagcctgt caagttcgct gtggatgagg gctatgcttt tgcccaggtt
     1621 gcgattgtca gttccgaaat agatggttac caggccggtg tcggtgtagg ggttgtacat
     1681 ggattccaca ttgtaggcgt atccacgctt ttcgcgaagc gaaaggttca ggcgcgaatt
     1741 catcccctgc ccgccgagta tattgttgag catgaacaac cccattctgc gtttgtcatg
     1801 caggtcatat gcaatattgc caatgatgca gtggttctgc catgtgtcgg ttttcttttc
     1861 tttgctgaca ggattatata agtactcaga cctctttctt cttgtgtcat ttcctgatgc
     1921 tacatgtccg aagtatttct caaagagcgt gatgattttt ttgggagtga tcgctccgac
     1981 ggagcagaag accatttgtg aagttgaata atttctggcg ataaatctgc ggatcatgtc
     2041 ggctgagatg cctctcactg ttcccggcat gccaagaatg cttcttccga tgggctgttc
     2101 cgggaagatc atctcctcga attcgtcgaa gatatattcg gcggggctgt caagataaga
     2161 gttgatctcc tcgatgatca cctctttttc cttttcaatt tctgccgggg gaaatgtaga
     2221 agagaagagc aggtctgata tcagttcaac agcctttggg tagtgttccc tgaggaatga
     2281 tgcgtggatg gctgtctcct ccttggtggt ataggcgttg agttcaccgc ccacatcctc
     2341 aaggcggctc agaatgtggt atgatttacg gtgtgttgtg cccttgaaga gcatgtgttc
     2401 gatgaagtgg gcaacgccgt gttcctcagg gagttcatcg cgtgaaccgg cattgatgat
     2461 aatgccgcag tgtgccacgg cgcttgtcgt aggaaggtgt accagcctga tgccgttggg
     2521 aagggtgtgg agagtaatat ccatttttat cggaagaatt gacaaagata agaaggaatt
     2581 aaagattcgg aacagtgttg cggctatccc ttcgggtatg ctgacagtga cctttttaca
     2641 aagtaatccg gggtgttgta tgaaaaataa aaaagcagta tatttgccgt ccgaaatggt
     2701 accatagctc agatggtaga gcaacggact gaaaatccgt gtgtccccgg ttcgattcct
     2761 ggtggtacca cccaaagaga agcgcaatcc tcagaaaaac aacaggttgc gctcttttta
     2821 tatccaaatc tttacaacat ttcaacaacc gggagggtaa ctgcaccact gtagcatcat
//
LOCUS       DAOPBK010000247         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_27365_length_2878_cov_3.394261, whole genome shotgun sequence.
ACCESSION   DAOPBK010000247 DAOPBK010000000
VERSION     DAOPBK010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27365_length_2878_cov_3.394261"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="PLA17_10770"
     CDS             <1..1585
                     /locus_tag="PLA17_10770"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HPK85934.1"
                     /translation="LNMKNRFKEFKYTITTDPKDSGAESGIPEELYRQMGVLHERALK
                     GGNKNIEKLIRLIEQYPGVPQLKNYLSVAWMNTGNYEKAKAINRRIVQEHPDYLFGRL
                     NLAFEYYFNKQYEKIPEVMGKMMEIQDLYPDRDCFHISEVTGFNRLAIMYFCAIGNLE
                     AAESRYEILENIAPGHPDTEDVFTYLMRARMKKAAKRMKEEGKTKIRVSSFGPDTSKQ
                     TRIKPEFSNSKIEWLYENGLEIDTWKFKELLLLPHDSLISDLRKVVKDCILRYHYFRS
                     LSRKAKEWPENILTFPIHAIYLLGELRAEEALGDVLETFGQSEEFLEFWYGDFLTGSL
                     WEPLFYMAEKRLNQLEDFVLSPNIYVYARSEVSCCVSQIGLHYPERRTEVVEWYRKVF
                     RRLAVAAPDEGLVDSDFIGLAISDALDLRAFQILPEIEALFNLGYVSQGICGKLSEVK
                     QEIKTPGRRIFKKKLLNIYDRYKQIKAEFTIHGKGIDDQDEYQTSPRKNSREPGRNDP
                     CPCGSGKKYKKCCLNNNRD"
     gene            1651..2304
                     /locus_tag="PLA17_10775"
     CDS             1651..2304
                     /locus_tag="PLA17_10775"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPK85935.1"
                     /translation="MNFHYGLSNGQLEIKREQKQPEGEEEQQQHEGKREQAQHEGKED
                     RSSVKKKESQHSIKENGNKPGLKEKFIKVAAELFSTSGTIRCKTLSVATGVFIGLSPV
                     WGFQTVTAIAASYLFRLNKILTIGFSAISLPPLIPLIIYGSYKLGGVILGNRAIDIDY
                     NSIKDFKEITTGVLQFVTGSLVMALIVSSVAGLTTYLILRWRERKRKSKTEHCCHMN"
     gene            2322..2471
                     /locus_tag="PLA17_10780"
     CDS             2322..2471
                     /locus_tag="PLA17_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85936.1"
                     /translation="MEGYLNIIPEKQADNSVDDYMVFLERCIERLREMIALIACLIDI
                     ARTEA"
     gene            complement(2495..>2878)
                     /locus_tag="PLA17_10785"
     CDS             complement(2495..>2878)
                     /locus_tag="PLA17_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPK85937.1"
                     /translation="AARSETEKPAIDDGMTAKTTEMKENAEVKTQTAAQAQPEPEMQQ
                     QAQPEAETRQQAQQQASSAGKSDDKPKPVQPERRKVHKVQRGESLADIAKKYNITVQE
                     LKKANKGVIFAMPDMRLVIPPSKEE"
BASE COUNT          829 a          623 c          699 g          727 t
ORIGIN      
        1 actcaacatg aagaacagat ttaaggagtt caaatataca attaccactg acccgaagga
       61 ctccggcgct gagtccggca tcccggagga actttaccgg cagatgggcg tccttcacga
      121 aagagccttg aagggcggga ataagaacat tgaaaaactg atcaggctaa ttgagcagta
      181 tccgggtgtc ccacagttga aaaactatct ttctgttgcc tggatgaaca ctggcaatta
      241 tgaaaaagcg aaggcaataa atcgtcgtat tgtacaggaa catcctgatt atctgtttgg
      301 aagattaaac ctggcttttg aatattattt taataagcaa tacgaaaaga ttccggaggt
      361 gatgggaaaa atgatggaga ttcaggatct gtatcccgac agggattgct ttcatatttc
      421 agaagtcacc ggtttcaaca gacttgccat aatgtatttc tgtgcgatag gcaacctcga
      481 ggctgccgaa tcccgttatg aaatccttga gaatattgcc cccggtcacc ctgacactga
      541 ggatgtcttc acctatttaa tgagagccag gatgaagaaa gcagcaaaga ggatgaagga
      601 ggaaggaaaa acgaaaatac gtgtatcctc tttcggtccg gacacctcaa aacaaaccag
      661 aatcaaacct gaattttcaa acagcaaaat agaatggctg tatgaaaatg gtcttgaaat
      721 agatacctgg aagtttaagg aacttcttct cctgccgcat gactccctta tctcagatct
      781 gagaaaggtt gtcaaggatt gtattctccg ctatcattat ttcagatcat tatcccgcaa
      841 ggctaaagag tggcctgaaa acatcctgac ctttccgatt catgcaattt acctgcttgg
      901 agaattaagg gctgaggagg cccttggcga cgtccttgaa acctttggtc agagtgaaga
      961 attccttgag ttctggtacg gagattttct gactggcagt ttgtgggaac ctcttttcta
     1021 catggcagag aagagactga accagttgga ggattttgtt ctctctccca atatctatgt
     1081 ctatgcaaga agtgaagtgt catgctgcgt gagccagata gggctgcatt atcctgaaag
     1141 aagaactgag gtggttgaat ggtaccggaa ggtattcagg cggcttgcgg ttgcagctcc
     1201 cgatgagggt ctggttgaca gtgactttat aggtcttgca atatcagatg cccttgatct
     1261 cagagcgttt cagatcttac ctgaaattga ggcgcttttt aatctcggtt atgtcagtca
     1321 aggcatatgt ggaaaactga gtgaagttaa acaggagatc aaaactcccg gaaggagaat
     1381 attcaaaaag aaattgctca atatatatga cagatacaaa cagataaaag ctgagtttac
     1441 tattcacggt aaagggattg atgaccagga tgagtaccag acatcccccc ggaaaaactc
     1501 ccgggaaccg ggcaggaacg acccgtgtcc gtgcggcagc ggaaagaagt acaagaaatg
     1561 ctgtcttaat aataaccgtg attgaaacag ttgctgccgg gacggtcagt atgccaatcc
     1621 tgtttattga acaaacaaac ataaccattc gtgaactttc attatggtct ttccaatgga
     1681 cagcttgaaa taaaaagaga acagaagcaa cctgaaggag aagaagagca gcaacagcat
     1741 gaaggaaaaa gggaacaggc acaacatgag ggaaaagaag acaggagcag cgtgaagaaa
     1801 aaggagagcc aacacagcat aaaggaaaat gggaacaagc ccggcctgaa ggaaaagttc
     1861 ataaaggtag cggctgagtt attctcaacc tcgggcacta tcaggtgcaa gacactttct
     1921 gttgcaacgg gtgtattcat tggcctctct ccagtatggg gatttcagac tgtgactgca
     1981 attgcagcaa gttatctttt caggcttaac aagatattga ccataggctt cagtgccata
     2041 agcctgccac cgctgattcc tctaatcatc tacggaagct acaagctcgg aggagtgatt
     2101 ctcggcaacc gggcaattga tatcgattat aacagtatca aagacttcaa agagatcacg
     2161 acaggggtgc ttcagttcgt gactggcagc ctcgttatgg cacttatcgt atcatctgtt
     2221 gccggactga ccacatacct gatactccgg tggcgggagc gtaaaaggaa gagcaaaacc
     2281 gaacactgct gtcacatgaa ctgaaatcgc caatcaacgt ggtggagggg tacctgaaca
     2341 tcattcctga aaaacaggct gacaactcgg ttgatgatta catggtgttc cttgagagat
     2401 gcatagagcg cctccgggag atgatagccc tcatagcctg cctgatagac attgccagaa
     2461 cagaggcctg aaagaagaga catactcagt caaactactc ctccttgctt ggcgggatga
     2521 ccagcctcat gtcaggcatg gcaaatatta ctcccttgtt ggccttttta agttcctgta
     2581 cggtgatatt atacttcttt gctatatcag caagagactc tcctctctgc accttatgaa
     2641 cctttcttct ctcaggctgg acgggctttg gcttgtcatc actcttaccg gctgaagatg
     2701 cctgctgctg agcctgttgg cgcgtttctg cctcaggctg agcctgctgc tgcatttctg
     2761 gttcaggctg cgcctgtgct gcagtctggg tctttacttc cgcattctct ttcatttccg
     2821 tggttttggc agtcatgcca tcgtcaattg ccggtttttc ggtctcactc ctggcagc
//
LOCUS       DAOPBK010000248         2863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_27555_length_2863_cov_4.567308, whole genome shotgun sequence.
ACCESSION   DAOPBK010000248 DAOPBK010000000
VERSION     DAOPBK010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27555_length_2863_cov_4.567308"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLA17_10790"
     CDS             <1..710
                     /locus_tag="PLA17_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPK85938.1"
                     /translation="VPTYQLSYFNDMEELARPIVNPRTQKLIKPFDKLYVRVLSIDPQ
                     TNQIFNSTDEVRLGSYSNTAGLIGYLVDEDGNISFPFVGKINVMDLTTADAADKIQKA
                     LSDYVPQTSIIVKYVDNQVTVMGEVQQQGIYSFSQDKLNIYEAIGLGGGITRYGDRKK
                     VILIRNEDGKVSHYALNLSDSKIANKSYYYVMPNDVIVVEPLKAISTSYSNITYTTIL
                     STISTAIAVLLFAGLRF"
     gene            725..>2863
                     /locus_tag="PLA17_10795"
     CDS             725..>2863
                     /locus_tag="PLA17_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85939.1"
                     /translation="MAHNINNDLQSEAFNIKEFITEALSYKYLYIASFFFCLLVAFLV
                     NRFSPTVYQVNSVIGPVQDRRSSMLRSNDMFLGLGALAESRNLENDINSLNSFRLVAT
                     TIKNLNLEIGYFAGKNSFLQKPAQVYLGNPYTVSIDKSHIQPINTRFRIEILDDHSYR
                     LTAYEDEASFYNYIDNAIVSRKNVFKIDTICRFNETLSAYNFKFSVTLNRDFYNPQTA
                     KETGYYFEFYHLDQLTKSYLKRLKVEPVSIRSSLINLFFSGENKRLTIDFLNSFIQAY
                     LDDNLSKKNKIALNTVNFIDLQISEISDSLSISESKLRDYRSTHQVTDLSYQGQQALE
                     QLRQIENERSMLQVQERYYNYILDYMEKNQDMAGLPTPSGANVSDPIMNSLVLEMLAL
                     NAERSAILSNKAEKNLFLNQIENKIRLQKQTIIENVKNNLNTLNLTQNELNYRESKLS
                     GEISRLPRTELNMVSMQRQFNLTDAIYTFLLEKKAEAAIAMASNYPDYEILEPARDVT
                     AGIIAPRSKLNWIIALFMALMLPTLFIILRNFFNEKISSVRDVERMIGQPVMNLIYTN
                     YYKTESVVMDHPGSSIAESFRNLRSSLFLKFRSEPHKVLMITSSQPQDGKSFVSSNLA
                     ASIASVGHKTILLDCDLRRPTLHDKFKVVNSVGLSNYMVNDVTSREIIKKTSSDNLWF
                     IPAGPILPNSSELIEAGALDNLIEELEQQFD"
BASE COUNT          867 a          677 c          587 g          732 t
ORIGIN      
        1 gtgtaccaac ataccagctg agctatttca atgatatgga agagttagcc cggccaattg
       61 tcaaccccag gacacaaaaa ctgataaagc cctttgacaa actttatgtc agggtactca
      121 gtattgaccc tcagacaaac cagattttca attcaacaga cgaagtgagg cttggatcat
      181 acagcaacac ggcaggtctt atcgggtatc ttgtagatga agacggcaat atcagttttc
      241 ctttcgttgg caagatcaac gtcatggacc tcaccacggc agatgctgcc gataagattc
      301 agaaagccct gagcgattat gttcctcaga cttctatcat tgtgaaatat gttgacaacc
      361 aggtgactgt tatgggagaa gtccagcagc agggcatcta ttcattctcg caggacaagc
      421 tgaacattta tgaggccata ggtctgggag ggggcataac gcggtatggc gaccggaaaa
      481 aagtcatcct tatcaggaat gaagatggca aagtgtcaca ttatgctctc aatctctctg
      541 actcaaagat agcgaacaag agttactact acgtaatgcc caatgatgta attgttgtgg
      601 aacctcttaa agccatctca accagctatt caaatattac ctatacaaca atactatcaa
      661 caatctcaac ggccatcgcg gtactccttt tcgctggcct gagattctaa acacttcttt
      721 ccggatggca cacaatatca ataatgattt gcagagtgaa gcctttaata taaaggaatt
      781 cattacagag gctttatcat acaagtacct ctacatcgcc agcttcttct tctgtctgct
      841 ggttgcattc cttgtaaaca ggttttcacc aactgtttac caggtcaatt cggtgatcgg
      901 gccggttcag gacaggcgct catccatgtt gaggtcaaac gatatgttcc ttggcctcgg
      961 ggcactggca gaatcgcgta acctggagaa tgacataaac agcctgaact ccttcagact
     1021 ggttgccaca accattaaaa acctgaacct ggaaattgga tattttgccg gcaaaaacag
     1081 ttttctccag aaaccggcac aggtttatct cggcaaccct tacactgtca gtattgacaa
     1141 atcgcatata cagccaataa atacaaggtt ccgcattgaa atactggatg atcattcata
     1201 ccgcctgaca gcttatgaag atgaggcatc attctacaat tatattgaca atgccatagt
     1261 cagccggaag aatgttttca aaattgacac tatatgccgg tttaatgaga cactctctgc
     1321 atataacttt aaattctccg ttacgcttaa ccgtgatttt tataatccac agactgctaa
     1381 ggagaccggt tactatttcg agttctatca tcttgatcag cttaccaagt catatcttaa
     1441 gcgactcaag gtggagcctg tcagtatcag gtcatccctc ataaatctct ttttcagcgg
     1501 tgagaataaa aggcttacca ttgatttcct gaatagcttc atccaggctt atcttgacga
     1561 caatctttcc aaaaagaata agatagccct gaatacagtc aattttattg acttgcagat
     1621 atctgaaatc tcagattcac tctctatatc tgaatcgaaa ttgcgtgatt accggtcgac
     1681 acaccaggtt actgacctca gctaccaggg acagcaggcg cttgagcagc ttcgtcagat
     1741 agagaacgaa agatcaatgc ttcaggtaca ggagagatat tacaattaca tccttgacta
     1801 tatggagaag aaccaggata tggccggtct gcccacacct tccggggcaa atgtgtcgga
     1861 ccctatcatg aactccctgg tacttgagat gcttgcactg aatgcagaaa ggtcagctat
     1921 tctcagcaac aaagctgaaa agaacctatt tctcaaccag attgaaaaca agatccgcct
     1981 tcagaaacaa acaataattg aaaatgtaaa gaacaacctg aacactctta acctgacgca
     2041 aaacgagctt aattaccgtg agagcaagct gtcaggcgag atatccaggc tgccccgcac
     2101 ggagctgaac atggtcagca tgcagaggca gtttaacctt actgatgcca tatatacctt
     2161 ccttctggaa aagaaagctg aagcggccat tgcaatggca tcaaattacc cggactatga
     2221 gatactggag ccggcaagag acgtaaccgc cggaattata gccccaagaa gcaagctcaa
     2281 ctggatcatt gccctcttca tggctttaat gttacctacc ctgtttatca tccttagaaa
     2341 tttctttaat gagaaaatat cttctgtcag agatgttgaa aggatgatcg gacagccggt
     2401 aatgaacctg atatacacca actattataa aaccgaatct gtagtcatgg accatccggg
     2461 gtcatcaatt gcagagtcgt tcagaaacct gcgcagcagc ctcttcctga agttcaggtc
     2521 tgaaccgcat aaggttctta tgatcacctc atcacaacca caggacggca agagttttgt
     2581 ttcaagcaac ctggctgcat caatcgcctc ggtaggacat aaaaccatac tgctcgactg
     2641 cgacctgcgc agaccgacac tccatgacaa attcaaagta gtcaactccg tcggcctctc
     2701 taattatatg gtcaatgatg tcacaagcag ggagattata aagaaaacct cctcggataa
     2761 cctttggttc atcccggcag ggccgatcct cccgaactca tcagaactga ttgaggcagg
     2821 agcgctggat aaccttattg aagagctgga acagcagttt gat
//
LOCUS       DAOPBK010000249         2829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_27977_length_2829_cov_5.688897, whole genome shotgun sequence.
ACCESSION   DAOPBK010000249 DAOPBK010000000
VERSION     DAOPBK010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27977_length_2829_cov_5.688897"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="PLA17_10800"
     CDS             complement(<1..1708)
                     /locus_tag="PLA17_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPK85940.1"
                     /translation="MKSLIQFFEENVEKYASNIYMWEKLQEKYEGTTYQETRRQVHEF
                     GAGLMQLGIKKGDRVSILADGRNSWVIGELGILYAGGVNVPLSIKLNPEEIKFRLNHS
                     ETRMVITSSTQAPKILAVAEKCPDLQKIIFMDPREEYAEGQIHFNEVRKIGREWLENP
                     ANRKSFEEFANSITASDFANICYTSGTTADPKGIILTHGNYVTNVYQSYSLMDIPPFY
                     KTLLILPWDHSFAHTAGIYAFMGKGASIASVKVGKTPMETLRNIPICMKEIKPNLLMS
                     VPALAKNFRKNIEKGIKGKGRMTEALFNFALKMAYSYNKEGWNKGRGIQKIKKPLLRL
                     FDKILFSKVREAFGGELDFFIGGGALLDIELQRFFYAIGVPMYQGYGLSEASPIISSN
                     SSARHKLGSSGFLVNNMDLKICDDNGNELPVGQKGEIVIRGGNVMHGYWKNDAATSEA
                     LRDGWLYTGDMGYMTADGFLYVLGRFKSLLIADDGEKFSPEGIEEAISEQSKYIDQCM
                     LYNNQRPYTVALIVPNQHAMKLYLERHNMSATTEEGKSAVLNLLENEVNEYRINGKYG
                     HMF"
     gene            1852..2319
                     /locus_tag="PLA17_10805"
     CDS             1852..2319
                     /locus_tag="PLA17_10805"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPK85941.1"
                     /translation="MKRLSFVLLAAVLLLTPSCDFMKSINPFGKKAREAEALRQQQEA
                     FRIADSIRVAKERQAEAERIRQAELAMEAEEQARAKTGYHVIVGSFLTPEYADAWLEH
                     IKGFGYDAQLVYMNGGRWHLVSAQVFPDLHKAWNALGSVQDRIDGDAWVYRPE"
     gene            complement(2382..>2829)
                     /locus_tag="PLA17_10810"
     CDS             complement(2382..>2829)
                     /locus_tag="PLA17_10810"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPK85942.1"
                     /translation="SGPFPTELNDADGQKMRDQGNEYGATTGRPRRCGWLDLPALKYA
                     IMVNGVTQLFMMKADVLSGFSEIKVCNSYTVNGKEQSELPYCLYDISELNYRSFRGWQ
                     CDITGCTTREELPEELKDYVRYIEDETGIPVTIISVGPDRSATITG"
BASE COUNT          636 a          710 c          704 g          779 t
ORIGIN      
        1 ggaacatgtg gccgtacttg ccgttaatgc ggtattcatt cacttcgttc tctagaagat
       61 tgagaacggc gctcttgcct tcctcggtgg tggctgacat gttgtgtctt tcaaggtaga
      121 gcttcatggc atgctggttg ggcactatca gggctacagt gtatggcctc tggttattgt
      181 agagcatgca ctggtcaatg tatttcgatt gttcgctgat ggcctcttca attccttccg
      241 gactgaattt ctcaccgtca tcagctatca ggaggctctt gaagcggccg agcacgtaca
      301 ggaaaccatc tgcagtcatg tatcccatgt cgccggtata gagccagcca tcacgcaggg
      361 cttcacttgt agcagcatcg ttcttccagt atccatgcat cacatttcct cctcggatga
      421 ctatctctcc cttttgtcct acaggcaact cgttgccgtt gtcatcacat atcttcaggt
      481 ccatgttgtt gaccaggaag cctgatgatc ccagtttatg cctggcgctg gagtttgacg
      541 agatgatggg tgatgcctcg gagagtccgt atccctggta cattggcact cctatggcat
      601 agaaaaagcg ctgcagttcg atgtcaagca gggcgccgcc gccgatgaag aaatcgagtt
      661 cacctccgaa ggcctccctt accttgctga aaagtatctt atcaaagaga cggaggagag
      721 gtttcttgat cttctgtatt ccgcgcccct tgttccagcc ctccttgtta taggaataag
      781 ccatcttgag agcaaagttg aagagggcct cagtcatcct gccctttccc tttatcccct
      841 tttcaatatt cttcctgaaa tttttcgcca gcgccggcac actcatcagc aggttgggct
      901 ttatctcctt catgcagatg ggaatgttgc gcagagtctc cataggggtc ttgcccacct
      961 tcaccgatgc aatgctggct cccttgccca tgaaggcata tatgcccgca gtatgggcaa
     1021 atgagtgatc ccatggaagg atgagcagag ttttgtagaa cggcggtatg tccatcaggc
     1081 tatatgactg gtaaacgttg gtcacatagt tgccgtgtgt gaggattatt cccttgggat
     1141 cggctgttgt gcctgatgtg tagcatatat ttgcaaaatc ggacgcagtg atggaattgg
     1201 caaactcctc aaagctcttc ctgttggcag gattttcaag ccactcacgc ccgatcttcc
     1261 tgacctcgtt gaaatgtatc tgcccttcag cgtactcctc gcgcggatcc atgaagatga
     1321 tcttttgcaa atcggggcat ttttctgcca ctgccagtat tttcggagcc tgtgtgcttg
     1381 aagtgattac catcctggtc tcggaatggt tgaggcggaa tttgatctct tcaggattca
     1441 gcttgatgga gaggggtaca ttgacaccgc cggcatagag tatcccaagt tcacctatga
     1501 cccagctgtt acggccatct gcgaggatgc tgacacggtc acccttcttt atgcccagtt
     1561 gcatcagacc ggctccgaat tcatgaacct ggcgcctggt ctcctggtaa gtggtacctt
     1621 catatttctc ctgaagcttt tcccacatgt aaatgttgga cgcatatttt tctacgtttt
     1681 cttcaaaaaa ctgaataagt gacttcatcg tgttaagtag tttgatggtt taaaacagtg
     1741 acctaatata ggaaagaaat atctgcgaac ggcatcttta ccgaaaattg attgtatttt
     1801 taaccataaa tccatacctt tatcacccaa aatcgtgaaa aactgccatt tatgaagaga
     1861 ctatcattcg tgttgctagc agctgttctg cttcttactc cttcatgtga tttcatgaag
     1921 agcataaatc ccttcggtaa aaaggccagg gaggcagaag ctctccgtca gcagcaggaa
     1981 gcatttcgca tagctgactc aatccgtgtg gccaaggaga ggcaggcgga ggctgaacgt
     2041 atccgccagg cagagctggc aatggaagct gaagaacagg ccagggctaa aaccggctac
     2101 catgtcatcg tgggcagttt tcttacccct gaatatgcag atgcatggct ggaacatatt
     2161 aaaggctttg gctatgatgc acagctggtt tatatgaatg gcggcagatg gcatctcgtt
     2221 tcagcacagg tcttccctga tctgcacaag gcatggaatg cgctcggcag tgtccaggac
     2281 aggatcgatg gtgatgcctg ggtctacagg ccggagtgac aattgcctgg ttgtccggga
     2341 cagaatcgat ggcaagcccg ggctaacggc cggagtgacg attacccggt gatggtggct
     2401 gacctgtcag gccccacgga aataatggtt acgggaatac ccgtctcatc ttcaatatac
     2461 cttacataat ccttcagctc ttcgggcagc tcctcccggg tcgtgcagcc tgtgatgtca
     2521 cattgccatc ccctgaagct cctgtagttg agttcactga tgtcatagag gcaatagggg
     2581 agttccgact gttcctttcc attcacggtg tatgagttgc atacctttat ctcactgaac
     2641 cctgagagta cgtcagcctt catcatgaac agctgcgtca ccccgttgac cataattgca
     2701 tatttcagtg cgggcaggtc aagccatccg cagcgtctgg gccttcctgt tgtggcgccg
     2761 tactcattgc cctggtcacg catcttctga ccgtcagcat cgttgagctc agtaggaaag
     2821 ggaccgctg
//
LOCUS       DAOPBK010000250         2821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_28085_length_2821_cov_4.892625, whole genome shotgun sequence.
ACCESSION   DAOPBK010000250 DAOPBK010000000
VERSION     DAOPBK010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28085_length_2821_cov_4.892625"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLA17_10815"
     CDS             complement(<1..517)
                     /locus_tag="PLA17_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPK85943.1"
                     /translation="MERKRVFMLLAMMALAVMLIRAQEVKVPLRFDFYYSYEKMVEAM
                     KALNTAYPEFTKLDEVGRSEEGRIIWALTINNPKTGDPFSKPGVYVDGNIHGNEIQAG
                     EVCLYLANRLLTNYGKITEITDVVDRNVFYIIPVVNVDGRAHFFSDANTASTNRSLRI
                     PMDDDRDGLFDE"
     gene            complement(522..2366)
                     /locus_tag="PLA17_10820"
     CDS             complement(522..2366)
                     /locus_tag="PLA17_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPK85944.1"
                     /translation="MFFTRLSVTGGMMLLVALLLLPEPISATRKYSDPAAVNRSIEQL
                     RQANPSLVKVHKFAVTPGGREMLMIEIGKSGTSVPAVLVGANFSGLTPLATEAAMSLA
                     SRVVSDASLNGKLTWYILPIGNPDAYARFFTRPLWSDSGNGRPFNDDTDDQTDEDGYN
                     DLDGNGIITRMRVKAHDGTWVPVEGEPRLMRKADAAKGERGIYKLYTEGIDDDGDGQY
                     NEDVPGGTNINNNFPHLFKKFGRRSGLYPGSEPESEAMLRFAFSHPEIAMVIAFGQTN
                     YLLSPPKGGRKGSIDTGSIRIPREMGASLGIDVSRTYTLAEVIELVQPMLPPGMTADE
                     SMIASFLGLGEVVNPMPDDMIFYNEISSDYKEYLKKKGITAERLDPAQPEEGSFELWA
                     YYHLGVPVFTIDFWGLPKPKEEKKENQGDEKKNDTVKADQQKGDPKEVAQLAFSDKWL
                     DGRGFTPWKSYKHPTLGEVEIGGFTPFSDNTPPENMIDSLLDLQLPYIPELVKRLPRL
                     AIAEVKTTEKGGGVYQLEAWVTNEGYLSFPIAMGKRNKVPAPAILTLEGDGIEILSGK
                     KRTPIGEVGGMKSVKYVWLVRSPKKETLTLRLESKQAGNDSKTTNIGG"
     gene            complement(2394..>2821)
                     /locus_tag="PLA17_10825"
     CDS             complement(2394..>2821)
                     /locus_tag="PLA17_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85945.1"
                     /translation="LPGEDPASTMMWTLVGFPLTTVAVPVWVSAGKTLPAAVSMKDNM
                     HAPLCDAAMTLKNQLFPIKRGSGPKYMNVALLLNKEKTGILQKVESVEKEIFMKTAAL
                     VAALPAKGKQQKEALLEHYKWLDGYITESYKELFGIEVK"
BASE COUNT          603 a          795 c          680 g          743 t
ORIGIN      
        1 cctcatcaaa cagcccgtcg cggtcgtcat ccatcggtat ccgcaggctc ctgttggtgc
       61 tcgcagtatt tgcatccgag aagaaatgcg ccctgccgtc gacatttacc actggtatga
      121 tatagaacac gttgcgatct acgacatcag ttatctcagt aatctttccg tagtttgtca
      181 gcagcctgtt ggcaaggtaa aggcaaacct cccccgcctg gatctcgtta ccatggatgt
      241 tgccgtcgac atacactccc ggcttggaaa atggatctcc ggtcttgggg ttattgattg
      301 taagcgccca gattatcctt ccttcttcgc ttcgacctac ctcatcaagc tttgtgaatt
      361 caggatatgc cgtattgagt gccttcattg cctcaaccat cttttcatag ctgtaataga
      421 aatcaaagcg gaggggcacc ttcacctcct gcgcccggat gagcatgacg gcaagagcca
      481 tcatcgccaa taacatgaat actctctttc tttccatggc tctaccctcc gatgtttgtg
      541 gttttactgt cattacctgc ctgtttcgac tccagcctga gtgtcagtgt ctctttcttc
      601 gggctcctga ctagccagac atacttgact gatttcatgc ctcccacctc accgatggga
      661 gtcctcttct ttccggaaag gatttcaatt ccatctcctt cgagggtcag tatggcgggt
      721 gccggcacct tgttccgctt gcccatggct ataggaaagg agaggtaacc ctcattggtc
      781 acccatgcct caagctggta aacaccgcct cccttttcgg ttgttttaac ctcagcgatg
      841 gcaagccgcg gaagtctttt caccagttcc gggatgtacg gaagctgaag gtccaacagc
      901 gagtcaatca tgttttccgg gggcgtattg tcactgaacg gagtaaaccc gcctatctcc
      961 acctcgccaa gggttggatg tttgtagctc ttccagggag tgaatccgcg gccatccagc
     1021 catttgtcag aaaaagctag ctgtgccacc tcctttgggt cacccttctg ctggtctgcc
     1081 ttcaccgtat catttttctt ttcatcgccc tggttctcct tcttttcctc ctttggtttc
     1141 ggaaggcccc agaaatcgat ggtgaacaca ggcaccccga gatggtaata tgcccacaac
     1201 tcaaatgatc cctcttccgg ctgggccgga tcaagccttt cagcagttat gcccttcttc
     1261 ttaaggtact ctttgtaatc tgacgatatc tcattgtaaa atatcatgtc atcaggcatc
     1321 ggattaacca cctccccgag gccaagaaag ctggcaatca tgctctcgtc agccgtcatg
     1381 ccggggggca gcatcggctg caccagttca atgacctcgg ccagggtata ggtgcgtgag
     1441 acgtcaatac cgagtgaggc acccatctcc cgtggtatac ggatactccc ggtatctatc
     1501 gagccctttc tgccaccctt cgggggcgaa aggaggtagt tggtctgacc gaaagcaata
     1561 accatggcta tttcgggatg cgagaatgcg aacctgagca tcgcctcaga ctcaggctcc
     1621 gaaccgggat acagtccgct gcgcctgccg aattttttga aaaggtgagg gaagttattg
     1681 ttgatgttcg tgccgccagg cacatcctcg ttatactgac catcaccgtc gtcatcaatg
     1741 ccttcggtgt agagcttata aattccccgc tcgccctttg cggcatcagc ctttcgcatc
     1801 aggcgcggct cgccctcaac gggtacccat gtgccgtcat gagccttcac cctcatccgg
     1861 gttatgatgc cgttgccatc aaggtcattg tagccgtcct cgtcagtctg atcgtcagta
     1921 tcatcgttga acggtctgcc attgccggaa tcagaccaga gcggcctggt gaagaaacgg
     1981 gcataggcat ccggattgcc gattggcaat atgtaccatg tcagcttacc gttcagcgat
     2041 gcgtcagaaa caacccgtga ggcaagtgac atagcagcct ccgttgcaag cggagtaaga
     2101 cctgagaaat tggcgcctac aagcaccgcc ggaacagaag tgcccgactt tccaatctca
     2161 atcatcagca tctccctgcc gccgggggta actgcgaact tatgcacctt caccagcgaa
     2221 ggatttgcct gccgtagctg ctctatcgag cggttcactg ctgccggatc actgtacttt
     2281 ctggttgcac tgatcggttc aggcaacagc agcaaagcga caagaagcat catgcctccc
     2341 gtcacggaca atctggtaaa aaacatcctg gaaatggttt tgcgtgttat ctgctatttt
     2401 acttcgattc cgaatagctc tttgtaagac tctgttatat agccgtcgag ccatttgtag
     2461 tgttcaagaa gagcctcttt ctgttgtttt ccctttgcag gcaatgccgc aaccagggct
     2521 gcagttttca tgaagatctc tttttccacc gattcaacct tctgcagtat gccggttttt
     2581 tccttgttga ggagcaaagc cacgttcatg tacttcggac cggagcccct tttgatgggg
     2641 aagagctggt tcttgagagt catggctgca tcacagaggg gagcgtgcat attatccttc
     2701 atgctcaccg cggccggcag ggtctttcct gcactgaccc acaccggtac tgctactgtc
     2761 gtcagaggga agccgacaag tgtccacatc atggtactgg ccggatcttc accggggaga
     2821 g
//
LOCUS       DAOPBK010000251         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_28137_length_2817_cov_2.570601, whole genome shotgun sequence.
ACCESSION   DAOPBK010000251 DAOPBK010000000
VERSION     DAOPBK010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28137_length_2817_cov_2.570601"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLA17_10830"
     CDS             complement(<1..602)
                     /locus_tag="PLA17_10830"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK85946.1"
                     /translation="MKRTIIFLLTAIAVVSCEFLDPRPIEDLTTDELLGNAVYGEGLL
                     AQAYAILNTEYIDYTEYYTDNAVPAVPGGNSLALGNWTVQGNPIGQWDAWYEAIGYLN
                     MYLRDCKNLLYSVSDPALNKILQKQRRGEAYFMRAWYQWLLLRTYAGYPAGGGECLGF
                     PIVTDVLSTSDNLDRPRNTYEECVAQIAKDCDSAIVLLPK"
     gene            complement(620..>2817)
                     /locus_tag="PLA17_10835"
     CDS             complement(620..>2817)
                     /locus_tag="PLA17_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85947.1"
                     /translation="DGYEAWYTPADLAAYQNHSNLLTHPDVDYYGEYVARAAPFRRAS
                     VSFEGGDRRVSYFSMFDYVGGSGLETVGEKVKNNRYKLRGNVAIKLNDFMNLSINIAA
                     SLNKQRFANISSGAGVYNMFDILSVYPSNAHPIYADSLLIRSDNFPVNLTNELAHSGF
                     GDGMILNAQNNARLVIDLGSIVEGLTAFGNASFDVSNSIINSKGGTEALYRLQGVTPV
                     RVVEKVVVPNMSLGSYNVLKRTAGYAGLNFEREFGKNSVAADAVFFAGLEEITSNDDW
                     YQPSKVADLSFRLNYGFDDRYVAQLDLVYSGSNRMPAGQRFSLYPTAGLAWIASNEDF
                     LRSNTSVDYLKVYGSAGIMGINDYYLQGYNPFYLFETLWTYSGSWRSGVATQYGDYVN
                     IYRLVQTATEDFVLPKRRFINIGAQAQLFGKSLGIEVNYFNEKNYDKISNKGASTPSI
                     IGSPQFLPATNYGEDMRWGIDGLIQWNKSIGDFRYGIGGNALYIRGKYLVVDEPDALP
                     EYQKRTGTDMDAFVIYNAEGLYQTAGEITSRGVTQSWGALQPGDIRYADLNGDNVVDE
                     LDVEVTDYHSPRFFYGLNLSAGYKGFGIYLLGQGEADGSVMLANNRYFMINGTGQNYS
                     ELMLDRFPATNDYPRLTTVSQNNYQYSTFWVANAAYFRLKNVEISYTFPVTAIRKLAM
                     NDLKLYVRGTNLAAVSSLKKYSVDPENINAGITGYPLFRTITAGISCKF"
BASE COUNT          718 a          726 c          642 g          731 t
ORIGIN      
        1 cacttaggca gaaggacaat ggccgaatca cagtcctttg caatctgcgc aacgcactct
       61 tcataggtat tgcgcggacg gtcaagattg tcagaagtgc tcagcacatc agttactatc
      121 ggaaaaccaa ggcattcacc accgccggcc ggatagcccg cataggtcct cagcagaagc
      181 cactggtacc atgccctcat gaagtaggct tctcccctcc tctgcttctg gagaatcttg
      241 tttagagccg gatcggaaac actgtagagc agattcttgc agtccctgag atacatgttc
      301 aggtatccga tagcctcata ccaggcatcc cactggccga tggggttccc ctgaacggtc
      361 cagttgccaa gggcaagcga attgccgccc ggaacggcag gaacggcatt atcagtatag
      421 tactcggtat aatcaatata ctcagtattg aggattgcat atgcctgtgc cagcaatccc
      481 tctccataga cagcattacc aaggagctca tcagtggtga ggtcctcaat cggcctggga
      541 tcaagaaact cacatgagac cactgcaatt gccgtaagga gaaatattat cgttcgtttc
      601 attgtttcag tttttaacat taaaacttgc aggatattcc ggcagtgatg gttctgaata
      661 acgggtatcc tgttattccg gcattgatgt tctccggatc tacactgtat ttcttcagtg
      721 atgaaacagc tgccaggttg gttcccctta cgtagagctt caggtcattc attgccagtt
      781 tgcgtatagc cgtaaccggg aatgtgtaac ttatttcaac gttcttcagc ctgaagtaag
      841 ctgcatttgc aacccagaaa gtcgaatatt gatagttgtt ctgcgaaaca gtcgttagcc
      901 tcggataatc attggtagca gggaacctgt caagcatcag ctcagaatag ttctggccgg
      961 taccgttaat catgaagtac ctgttgttgg caagcatcac gctgccgtca gcctcaccct
     1021 gcccgaggag atatatgcca aagcccttgt atccggcgga gagattgagt ccgtagaaga
     1081 atctgggcga gtggtaatct gtaacctcca catcaagctc atcaacaaca ttatcaccgt
     1141 tgaggtcagc atacctgatg tcaccgggct gaagtgctcc ccagctctgg gttacacccc
     1201 ttgatgttat ctcacctgct gtctggtaga gaccctctgc attatagatt acgaaagcat
     1261 ccatatcggt accggttctc ttctggtact cgggcaatgc atcaggctca tccacaacaa
     1321 gatatttgcc ccttatgtat aacgcgttcc cgccgatacc atacctgaag tcgccaatac
     1381 tcttattcca ctgtatcaat ccgtcaatac cccatctcat atcctcacca tagttggttg
     1441 ccggaaggaa ctgcgggctg ccaatgattg aaggagtaga cgctcccttg tttgatattt
     1501 tgtcatagtt cttttcattg aagtagttca cttcaattcc aagtgacttg ccgaagagct
     1561 gtgcctgtgc cccgatgttg ataaagcgtc tcttggggag cacaaaatcc tcggtagcgg
     1621 tctgcaccag tctgtagata ttcacataat caccgtactg ggtagctacg cccgatctcc
     1681 agctgcctga gtaagtccac agagtctcaa aaaggtagaa cgggttgtag ccctgaaggt
     1741 aatagtcatt aatacccatg atgcctgctg atccgtaaac cttcaggtaa tctacagagg
     1801 tgtttgacct caaaaaatcc tcattgctgg caatccatgc aaggccggca gtaggataga
     1861 gtgaaaacct ctgccctgcc ggcatcctgt tgctgcctga gtaaaccagg tcaagctgtg
     1921 ccacatacct gtcatcaaaa ccataattca gtctgaaaga gagatctgcc acctttgaag
     1981 gctggtacca gtcatcattg gaagtaattt cctcaagacc tgcaaagaag actgcgtctg
     2041 cagcaacgga gttctttcca aactcacgtt cgaagttaag ccctgcatat ccggcagttc
     2101 tcttcagaac attgtaagat cccagtgaca tattgggaac cacaaccttt tcaacaactc
     2161 ttaccggagt tactccctga agcctgtaga gagcttcagt gccgcccttg ctgtttataa
     2221 tgctgtttga gacatcaaac gaagcatttc caaaggcagt aaggccttca actatgcttc
     2281 ccaggtcaat taccagcctg gcattgttct gggcgttcag aatcatgcca tcaccaaatc
     2341 cggaatgtgc cagttcgttt gtcaggttga ccgggaagtt atcactcctg atcaaaagtg
     2401 aatcagcgta aatgggatgc gcattcgacg ggtataccga aagtatatca aacatattgt
     2461 atacaccggc acccgacgag atattggcaa agcgctgttt gttcagcgat gcggcgatat
     2521 ttatgctgag gttcatgaag tcgttcagct tgatcgccac attgccgcgc agcttatacc
     2581 tgttgttctt taccttttcg cctaccgtct caaggccgga acctccaaca tagtcaaaca
     2641 ttgagaagta ggaaaccctc ctgtcgcctc cctcgaagct cacgcttgcc ctgcgaaagg
     2701 gtgctgccct ggccacatat tctccgtaat aatccacatc gggatgagtg agcaggtttg
     2761 aatggttctg gtaggcggcg agatcggcag gcgtatacca tgcctcataa ccgtcat
//
LOCUS       DAOPBK010000252         2786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_28549_length_2786_cov_2.946174, whole genome shotgun sequence.
ACCESSION   DAOPBK010000252 DAOPBK010000000
VERSION     DAOPBK010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28549_length_2786_cov_2.946174"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PLA17_10840"
     CDS             <1..1407
                     /locus_tag="PLA17_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85948.1"
                     /translation="FNQFGISPKYRWLTLHLGYRNLSFSEFTLSGYTFLGAGIEASPG
                     RFRIGAMYGKFNQNSVYDLAMADSMPKMTRTGWAAKLGYGSEKRFVDLSVLRIGDDPK
                     GYDTLNIEPGIPAPEQNIALGLTSRFDISEKLTFRFDGSYSFYTFNRAFAATGISDDA
                     LLRLAGNFIRVNSTSEYFKALKTSISYSFSQGTVTGIEYRRIDPGFRSMGSYFFNNDI
                     EHLTINQSLALLKNKINLRGSLGAQRDNLKGAKEHTALRIIGSLTGNYNINQNWAVDA
                     TFNNFSTNQRVFRTATDQSLLIYQVNRTITLSPRYMNAGEKMSHMAMLNINVTALEDK
                     NQSTSDMTETETYMAMLIYNLGLLQLKMNISAGLNYTMMLNKNFENRLAGGNINVAKT
                     LLRDQLSLGLNNNLMINRINDDKGLILNSALTAAWRFHPKHSLSLNLNLINNRFAEGS
                     AVPTYHEIRGDFGYAFTF"
     gene            1391..>2786
                     /locus_tag="PLA17_10845"
     CDS             1391..>2786
                     /locus_tag="PLA17_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TANFOR domain-containing protein"
                     /protein_id="HPK85949.1"
                     /translation="MRSHSDRSHEKEEKTMASATFRKDRFPVRYPETSCRESFKTSGQ
                     AAGYPASSRAVAFLRRGLAAVILIITALTAALAQELVNQTVQVLPPYTGKLSHYFATP
                     GRIVSIITTNPNNIDAREYRYYLHGYIESVHNDGEIRIGTRRDYRPATPSVMKAQLGP
                     DGGVIAWLPYTLTYSDIQQVFSPQNLEYRGISREQAEKTGLPDGMYRICFMLYINPWG
                     DGFSAQGPFCSPPFSIISDFTAVEAPQIIQPPDNSMLTPEQLQTLQFTWTMPPGAPAT
                     TQYRLRIIELNDESANYRDMLRTEAYPAFFETTVTGVPTFLYTIANPPFRPDKAYAFI
                     IRAIDPLGNTNFKNSGFSEVNLFMQAKQTMSSPPPAAPPPTLYIPGQQFTRVTPSIMK
                     PITIKGRLSYKYPSARPKLFSQGTKPPPLSNARIKLVTYYIVKDSKGRIVENMTREGQ
                     KWSNHVNYKGLDDRV"
BASE COUNT          768 a          793 c          646 g          579 t
ORIGIN      
        1 ttcaaccagt tcggaatcag tccgaaatac aggtggctca ctcttcacct gggctaccgc
       61 aatctgtcct tctctgaatt caccctgagc ggttacactt ttctgggtgc cggtattgaa
      121 gccagtccgg gcaggttccg catcggtgcg atgtatggta agtttaacca gaactccgtg
      181 tatgacctgg cgatggccga ctcaatgccg aaaatgaccc ggacaggctg ggctgccaag
      241 ctgggctacg gcagcgagaa acgatttgtc gacctgtcgg tgctgcggat aggtgacgat
      301 cccaaaggat atgacactct taatatcgaa ccgggaatac cggcacccga acagaatatt
      361 gcactgggac tcacctcccg ctttgacata agcgaaaaac tgaccttcag gtttgacggt
      421 tcatacagtt tctacacctt caaccgcgcc tttgccgcca ccggcatcag cgatgacgcc
      481 ctgctccggc tggcaggcaa tttcatcagg gtgaacagca ccagcgaata cttcaaggcg
      541 ctcaagacca gcatcagcta ttccttcagc cagggaaccg taaccggtat cgagtaccgg
      601 cgtatcgacc cgggcttccg gtccatggga agttatttct tcaacaacga tattgaacac
      661 ctgaccatca accagagcct ggctctcctg aaaaacaaaa ttaacctgcg aggctcactg
      721 ggggctcagc gtgataacct gaaaggggcc aaggagcata ccgcactcag gatcatcgga
      781 tccctcaccg gcaattacaa cataaaccag aactgggcag tagatgcaac attcaataat
      841 ttctcaacca atcagcgtgt attcaggaca gcaacagacc aaagcctgct catctaccag
      901 gtcaaccgca cgataacact gtccccgcgc tacatgaacg ccggcgaaaa aatgtcgcac
      961 atggccatgc tgaacatcaa tgttacagcg cttgaagaca aaaaccagag cacttcagac
     1021 atgactgaaa ccgaaaccta tatggcaatg ctgatctaca acctgggcct gcttcagctt
     1081 aagatgaaca tttccgccgg actcaactac accatgatgc tgaacaaaaa ctttgagaac
     1141 aggctggcag gcggcaatat caatgttgcc aaaacactgc tccgggacca gttatcactt
     1201 ggactgaaca ataacctgat gatcaacagg atcaacgatg acaagggtct gatcctcaac
     1261 tcggcgctga cagctgcctg gaggttccac ccgaaacatt cactctcact taaccttaac
     1321 cttatcaaca accggtttgc cgaaggatcg gcagtaccga catatcacga aataagagga
     1381 gactttggct atgcgttcac attctgacag atcacacgaa aaagaagaaa agacaatggc
     1441 atctgccaca ttccgcaaag accggttccc ggtacggtat ccggagacat cctgccggga
     1501 gagttttaag acatcgggac aggctgcagg atacccggca tcatcccggg ctgtcgcctt
     1561 cctcagaaga gggctggccg cagtgatcct gatcataacg gcgctgacgg ctgccctggc
     1621 tcaggagctg gtaaaccaga ccgtgcaggt gcttcccccc tataccggca agttgtccca
     1681 ttacttcgca acaccggggc gaatcgtgag tataatcacg actaacccta acaatataga
     1741 cgcaagggag tacaggtatt accttcacgg ctatattgag agcgttcaca atgacggtga
     1801 gatccggata ggtacccgga gggactaccg gccggccaca ccttcagtaa tgaaagctca
     1861 gctggggcct gacggaggag ttatagcatg gcttccctat acacttacct acagcgacat
     1921 acagcaggtc ttcagtcccc agaaccttga gtaccggggt atctcccgcg agcaggcaga
     1981 aaaaaccggc ctgccagacg gcatgtaccg tatctgtttc atgctctata tcaacccctg
     2041 gggtgacggc ttttctgcgc agggaccatt ctgcagccct ccgttcagta tcatctccga
     2101 ttttacagcc gtggaagctc cccagattat tcagccgccg gacaattcca tgctcactcc
     2161 cgaacagttg cagacactgc agttcacctg gactatgcct cccggtgctc ctgcaaccac
     2221 acagtacagg ctgaggatca ttgagctgaa tgacgagtcg gccaattacc gggacatgct
     2281 ccgcaccgaa gcttatcctg catttttcga gaccaccgta accggtgtac ccaccttttt
     2341 atataccata gccaatccac ccttcagacc tgacaaggct tacgcattta taatcagagc
     2401 aatagatccc cttggaaaca caaacttcaa aaacagcggc ttcagcgaag tgaacctttt
     2461 catgcaggct aaacagacga tgtcgtcacc accgcccgcg gccccgccac caacacttta
     2521 tattcccggg cagcagttca cccgggtgac cccgtcgata atgaagccca tcactattaa
     2581 aggaaggctg tcatataagt acccctctgc caggccaaag cttttttctc agggcacaaa
     2641 accaccgcca ctgtcaaatg ccaggatcaa actggtcaca tattacatag tgaaagattc
     2701 aaaaggacga atagttgaaa atatgaccag agagggtcag aaatggagca atcatgtcaa
     2761 ctacaaaggg cttgacgaca gagtgg
//
LOCUS       DAOPBK010000253         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_28741_length_2771_cov_6.173785, whole genome shotgun sequence.
ACCESSION   DAOPBK010000253 DAOPBK010000000
VERSION     DAOPBK010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28741_length_2771_cov_6.173785"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1552
                     /locus_tag="PLA17_10850"
     CDS             368..1552
                     /locus_tag="PLA17_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85950.1"
                     /translation="MNRGYRLAELSSKKDIRDFHDLPASINRGDKNWIKPLFKDIETI
                     FHPSSNKLLRTGEATRWLLRDDTGRTVGRVAAFYSKKIAGASGEQVGGMGFFECINDR
                     EAATILFDACRNWLEEKGMTVMEGPVNFGERDRWWGLLVDGFIPANYCMPYNPPYYRE
                     LFEAYGFRNYFNQYTYHMPVDDSRLDPVIRRKAERIFSNPDYTFRYMTLKEFRDIARN
                     FMIVYNKGWARFPGVKTISEGHAKILLKSIRPVIDEKLLWFGFYKDEPICFFIMLPEV
                     NQIVRHLNGKLNHVGRLKFLWYRHIKNELTKAFGLIFGIVPEHQRKGVEGALIMSFAK
                     LAMSDRFPYKELEFNWIGDFNPSMIHLLDQIGAKIIKTHITYRYLFDRNAPFERSPVV
                     NV"
     gene            complement(1610..2605)
                     /gene="trpS"
                     /locus_tag="PLA17_10855"
     CDS             complement(1610..2605)
                     /gene="trpS"
                     /locus_tag="PLA17_10855"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPK85951.1"
                     /translation="MDTVVSGIRSTGNLHLGNYFGALKNFLKMQDENNCFFFVADYHS
                     LTTHPHPDDIHGNIRQVLAEYLAAGIDPEKATVYVQSDMPEVTELYLLLNMISYVGEL
                     ERTTSFKEKIRKHPDNINAGLLTYPVLMAADIIIHRAHKVPVGKDQEQHLEMTRRIAR
                     RFNTMYGVDYFPEPDAYNFGKQLIKIPGLDGTGKMGKSEGNGIFLADTPADIRKKVMK
                     AVTDTGPEKPDAEPSEPVKNLFTIMEVVSEPSTLQYFREQHATCRIRYGDLKKQLAED
                     IIRVTDPIRIRINEILNDPDYLGKVVTRGAEKARASASKTVREVREIIGYRPFTH"
BASE COUNT          687 a          686 c          672 g          726 t
ORIGIN      
        1 ctcaggtaca ggccatactc ttcaggcata tcagcgccgg gaagaaattt tatcgtagct
       61 gccagaatca agatctgagc cccgcctgcc agtcattcaa tgctaaatgc cctgcggggt
      121 ggttcggttg ccttcgtgta tggcgagatg ctgccagtca ttcaatgtta aatgccctgc
      181 aggcatcata tgccataaca cacctgatct gcaatgcttt atgagctaca ctcatttgct
      241 gcagtggcag taagccggta aggattgcat caacatgtct atgaaaacaa ttgaatcctc
      301 ctcaccctca ttccttggat attcaaaatg aatgagtaat tttcagaaat tttggttttc
      361 agagggtatg aatcgtggtt acaggcttgc tgaactcagc agcaaaaaag acatcagaga
      421 ttttcatgat ctgccggcat ctatcaacag gggagacaag aattggataa agcccctgtt
      481 caaggacatt gaaacaatct ttcacccttc ctccaacaag ctgttacgta caggcgaggc
      541 aacaaggtgg ctgcttcgcg acgacacagg gcgtaccgtt ggccgggtag cggctttcta
      601 cagtaagaaa attgcaggag cctccggaga acaggtgggc ggaatgggct tttttgaatg
      661 cattaatgac agggaggctg cgacgatact gtttgacgcc tgccgcaact ggctggagga
      721 aaagggtatg accgttatgg agggaccggt caacttcggt gaacgtgacc gctggtgggg
      781 attgctggtt gacggcttca tacccgcgaa ctactgtatg ccctataacc cgccatacta
      841 cagggaattg ttcgaggcat acggattcag gaactatttc aaccagtaca cttaccacat
      901 gcctgttgac gacagcaggc tggatcccgt gatcaggaga aaggcggaaa ggatcttcag
      961 caaccctgac tatacattca ggtatatgac cctgaaagag tttcgtgaca ttgcccgtaa
     1021 ctttatgatt gtctacaaca aggggtgggc ccgcttcccg ggtgtcaaga caatcagcga
     1081 gggccatgca aagattctcc tcaagagcat cagaccggtg attgacgaga aactgctctg
     1141 gtttggcttc tacaaagacg aacccatctg cttcttcatc atgctccccg aggtcaacca
     1201 gattgtcagg catctcaatg gcaagctcaa tcatgtgggt cggttgaaat tcctgtggta
     1261 caggcatatt aaaaatgaac tgaccaaggc attcggcctg atcttcggca ttgtgccgga
     1321 acatcagcgc aagggggtgg aaggagccct gataatgtca ttcgccaaac tggcgatgtc
     1381 tgacaggttt ccgtacaaag agctggaatt taactggata ggcgacttca atccctcaat
     1441 gatacacctt cttgaccaga taggcgcaaa aataatcaag acgcacatca cctaccggta
     1501 cctgtttgac aggaatgcac ccttcgagag gtcgccagtg gtcaatgtgt gaatggcctg
     1561 tggccggtga tctttgattt tcgttgcgtc tattcgctgc tacacctggt cagtgcgtga
     1621 atggcctgta gccaatgatc tccctgacct cacggacggt ttttgaagcg ctggcccttg
     1681 ccttctccgc tcctcttgtc acaaccttgc ccaggtaatc cggatcattg agtatctcgt
     1741 tgatccttat ccttatggga tcagtgaccc tgatgatgtc ctcggcaagc tgcttcttca
     1801 aatcgccgta ccttatccgg catgtggcgt gctgctcacg gaaatactga agcgtggatg
     1861 gttccgaaac cacctccata attgtaaaaa ggtttttgac cggttctgac ggttcggcat
     1921 ccggtttctc cggtccggta tcggtaacgg ctttcatcac ctttttgcgt atatccgcag
     1981 gcgtatcagc caggaaaatt ccattcccct cactcttgcc catcttaccc gtaccatcaa
     2041 gacccgggat ctttatcagt tgtttgccga agttataggc atcgggctcc gggaaataat
     2101 caaccccgta cattgtattg aagcggcggg caatcctgcg ggtcatctcc aggtgctgct
     2161 cctgatcctt tccaaccggc accttgtggg ccctgtgaat tataatatcg gcagccatca
     2221 gcaccgggta ggtcagcagg ccggcattga tgttatccgg gtgcttgcgt atcttctcct
     2281 tgaatgaagt ggtacgctca agctctccca cataagaaat catgttcagc agaagataaa
     2341 gctcggtcac ttcgggcata tcgctctgca catagacggt tgccttctcc ggatcaattc
     2401 ctgctgccag gtattcggca agcacctgcc ttatattgcc atgaatgtca tcaggatgcg
     2461 gatgtgttgt aagtgagtga taatctgcta caaagaaaaa acaattgttc tcatcctgca
     2521 tcttgaggaa attcttcaga gctccaaaat agtttcccag gtgaagattg cctgttgacc
     2581 gtatcccgct tactactgta tccattaatt tattattagc agctgcaaaa atagctatta
     2641 ttcacgaatt catttcattt cctgcatgat cagctgcctg agtgccggta ccgaacttat
     2701 ttagtatttt tggggcataa ttcatccgca atggaatcga cacggcagaa gaaagttgca
     2761 cgtctggtgc a
//
LOCUS       DAOPBK010000254         2738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_29186_length_2738_cov_4.737980, whole genome shotgun sequence.
ACCESSION   DAOPBK010000254 DAOPBK010000000
VERSION     DAOPBK010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29186_length_2738_cov_4.737980"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="PLA17_10860"
     CDS             complement(<1..1396)
                     /locus_tag="PLA17_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPK85952.1"
                     /translation="MNRILLFALAILLITSCAQQRENEFFTARIDFPVQPIFGVKEAM
                     AARLIPHQRQLEWQKLEMTAFIHFSINTFTDSEWGTGKESPELFNPSSLDAEQWVRVL
                     NEAGMKMVIITAKHHDGFCLWPTKTTTHSVASSPWKDGKGDVIRELKDACEKYNMKFG
                     IYLSPWDRNAACYGDSPAYNRFYMEQLTELLTWYGRVDEVWFDGANGEGPDGRRQEYD
                     WQAYYDLIRKLQPDAVTAIMGNDVRWVGTESGYGRETEWSATVLAPGGTPESVAINEA
                     LGIDAMSEDLGSSSLLSRANHLFWYPAEVDVSIRPGWFWHASEDSLVKDVAKLSDIYL
                     SSVGRNAVLLLNVPPDNRGLITDHDINSLRGLKTWTDKLYATDLLNGSRPYGRGASNL
                     TDGDNSTSWNPRHNPVASFVPAIAATFNVAMLQEDTSKGQRVEKFIIEALSGTEWDTI
                     ATGTTIGYKRLIRFP"
     gene            complement(1405..1812)
                     /locus_tag="PLA17_10865"
     CDS             complement(1405..1812)
                     /locus_tag="PLA17_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85953.1"
                     /translation="MDDILKKLKYREQERIAVLNAPRKFLGRITRLLPGVQIDSEINA
                     RFLYDFMIAFTPGSAEVDKYGPACIHNLSEDGKLWMVYPKSSSRRYKSNINRNRGWET
                     VTDTGFKPVGQVAIDEDWSALRFRNVKFIKNGK"
     regulatory      1910..2005
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2060..>2738
                     /locus_tag="PLA17_10870"
     CDS             2060..>2738
                     /locus_tag="PLA17_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPK85954.1"
                     /translation="MLGKKALLMLASVFLTVIVTGQKSEVVGQKSGVAGQSSGVTIQK
                     SGVTGSGRNQGEMIRPQGQVETIRPQSQGNMITGRVTDCGGVPMAGASVVLTDAGMGV
                     ATDNEGRYALRGLSDGSYNLRISFTGYEPHDTLVVVRGSAVVDVVLNEVLYVAPEVIV
                     RGSRAGVRTPMAHSTIGSDELRKRDLTRDMPFLLALTPSVVETSDAGTGIGYTSMRIR
                     GTDASRIN"
BASE COUNT          616 a          700 c          741 g          681 t
ORIGIN      
        1 caggaaaccg tatcagcctt ttatatccga tagttgtgcc cgttgcaatg gtatcccact
       61 cagtgccgga gagagcctcg attataaatt tttccacccg ttgccccttg ctggtgtctt
      121 cctgcagcat tgccacattg aatgtagcgg ctatggccgg aacgaatgag gccacggggt
      181 tgtgacgcgg attccacgag gtgctgttgt ctccgtcggt aaggttcgat gctccccgtc
      241 cgtatggtct tgagccgtta agcaggtcag tggcatagag cttgtctgtc caggttttca
      301 acccccggag gctgttgata tcatgatcgg ttatgagccc acggttgtca ggcggcacat
      361 tgagcagcag aacggcattg cgccccacgg agctcaggta gatgtcagac agctttgcaa
      421 catccttaac cagtgagtct tcggaagcat gccagaacca gcccggtctg attgagacgt
      481 caacctctgc cggataccag aagagatgat ttgcccggct gagcaggctg ctgcttccaa
      541 gatcctctga catggcatct atccccagtg cctcgttgat ggcgacactc tccggggtgc
      601 ccccgggtgc cagtacggtg gcgctccatt cggtttcgcg gccgtagccg ctctctgttc
      661 ccacccatcg tacatcattc cccatgatgg cggtgacggc atcgggctga agcttcctga
      721 taagatcata gtatgcctgc cagtcatatt cctgccggcg gccgtcaggc ccttcgccat
      781 tggcgccgtc aaaccacacc tcatcaaccc tgccgtacca tgtcagcagc tcggtcaact
      841 gctccatgta aaagcggttg taggcaggcg agtcaccata gcatgccgca ttcctgtccc
      901 agggcgacag atagataccg aacttcatgt tatacttttc acatgcatcc ttaagctccc
      961 tgatgacgtc gcccttgcca tccttccacg ggcttgaggc aactgagtga gtggtggttt
     1021 tggttggcca gagacagaag ccgtcgtgat gctttgcagt gataataacc atcttcattc
     1081 cagcctcgtt gagtaccctg acccattgtt ccgcatcaag cgaggaggga ttgaagagct
     1141 ccggcgattc cttgccggta ccccattcgc tgtcagtgaa ggtattgata gagaagtgta
     1201 tgaatgccgt catctccagt ttctgccatt caagctgccg ctgatgaggg ataagccggg
     1261 cagccatcgc ctcctttacc ccgaaaatcg gctggacggg gaagtcaatc cttgcggtga
     1321 aaaattcatt ttcacgctgt tgtgcgcacg atgtgatcag taagattgca agggcaaaca
     1381 gcagaatcct gttcatcaga aaaattattt gccgtttttg atgaatttga cattccggaa
     1441 cctgagggct gaccagtcct cgtcaatggc tacctggccc acaggcttga acccggtatc
     1501 tgtaacagtc tcccatcccc ggttacggtt gatatttgat ttatacctgc gggaagaact
     1561 tttcggatag accatccaca gcttgccgtc ctcactgagg ttatggatgc atgcagggcc
     1621 gtacttgtca acttctgcag agcctggagt aaaggcaatc atgaagtcat acagaaagcg
     1681 ggcattgatc tcagaatcaa tctgtacgcc cggaagaagc ctggtgatgc ggccaaggaa
     1741 cttccttgga gcgttaagca ctgcgatacg ttcctgttca cggtacttca gttttttcag
     1801 aatatcgtcc atttcagtat attcttcccg gaacaaatat acaaataaca gagaattgca
     1861 attaccatga tccgccggtg gtaatttcac atttatttga tacctttgtg aggtcgattc
     1921 ggggtgcctt acttaacagg ctgagatcat acccttataa cctgatccgg ataatgccgg
     1981 cgtagggaaa gagagtaatc attttccagc ttgtctgaac acccctgatt cagatgatgt
     2041 taatttaaaa gctggagaaa tgttggggaa aaaagcgctt cttatgttgg cttccgtctt
     2101 tctgacagtg attgttaccg gtcagaagag tgaagttgtc ggtcagaaga gtggagttgc
     2161 cggtcagagt agtggagtta ccattcagaa gagtggagtt accggttcag ggcggaacca
     2221 gggagagatg attcgtccac agggccaggt agagacaatc cgtccgcaga gccaggggaa
     2281 catgatcacc ggcagggtca ctgactgtgg aggtgtgcct atggctgggg cttctgtggt
     2341 actcactgat gccggtatgg gtgttgccac tgacaatgag ggaaggtatg cccttcgggg
     2401 tctgagcgac ggatcctata acctgcgtat cagctttacc ggttacgagc cacatgatac
     2461 cctggtagtt gtgagaggct cggctgtggt tgatgttgtg cttaatgagg tcctttatgt
     2521 agccccggag gtgatcgtca ggggcagccg tgccggggtg agaacaccga tggcacatag
     2581 taccataggc tctgacgaac ttcgcaagcg tgacctgacc agggacatgc cgttcctgct
     2641 cgcgcttaca ccgtcagtgg tggagacctc cgacgccggg acgggcatag gctatacctc
     2701 catgcgcata cgtggaacag atgccagccg gatcaaca
//
LOCUS       DAOPBK010000255         2675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_30093_length_2675_cov_3.514504, whole genome shotgun sequence.
ACCESSION   DAOPBK010000255 DAOPBK010000000
VERSION     DAOPBK010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30093_length_2675_cov_3.514504"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLA17_10875"
     CDS             complement(<1..478)
                     /locus_tag="PLA17_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85955.1"
                     /translation="MENREQSFYIPESEGIDFRRYFSLFISNWYWFAAALFLTLAIAY
                     GVNRWGEELYTVQSTMLISDDEYGGGFAEMDKVIPGGDIFRPRQNIRNEIGILKSFDL
                     NHKVMYRLTDFHVVYTAVGRRGIAESRMYRNSPFVVNLDTTTVRFQPGKRVDIKILS"
     gene            complement(660..1469)
                     /locus_tag="PLA17_10880"
     CDS             complement(660..1469)
                     /locus_tag="PLA17_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPK85956.1"
                     /translation="MTYRPSDLRHAKTLFPLLALLLFMAGCATQKELSYLNNLEEVDG
                     ANQFPMEVPDYKVQPRDIIYVSVKAQTPEGTLTEMLSDRASTNMNYISNEGTSYMMGY
                     SINPQGYVTLPLLGDIPVAGLTIYEIRDLFQQKIDSLFRHAYVEVRLMSFKFTVIGEV
                     RAPGSYVNYNDQLTVLEAIGRAGGISETGTKEKILVIRPQGEQTLTYHIDLQDKSLLS
                     SPAYFITPNDVVIVQPNPKKVFSVNLPTISFILATVTSTISTTLLLINYFK"
     gene            complement(1495..2310)
                     /locus_tag="PLA17_10885"
     CDS             complement(1495..2310)
                     /locus_tag="PLA17_10885"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPK85957.1"
                     /translation="MEETQSQSVVTPPSGTASLSAAPPQAAPLTGAAPLSADSQSADT
                     MPGATPPQADTSPGTTPPETTPPQATPPQAAKPSASAPPQATPPQAATSQAPRELRRP
                     VKQWYAIYTNSRAEKRVHERLIEQGIESFLPLQKTLRQWSDRKKLVERPLISSYVFVK
                     IIQREYFTVRKTEGVVKFIMIQGKPIPIPEEQITNLRILCGSDADVEISAEVYEKGDM
                     VEVTVGSLTGLRGELIRVGRKHKVVIRVIQPGMNLTVDIKTNSIRKIGKNTDI"
BASE COUNT          552 a          678 c          761 g          684 t
ORIGIN      
        1 gtgagaggat ttttatgtcc actctcttgc ccggctggaa cctgacggtg gtggtgtcaa
       61 gattcaccac gaagggcgag ttgcggtaca tcctgctctc agctatgccc ctccggccaa
      121 cagcggtata gaccacgtga aaatctgtga gcctgtacat caccttatgg ttgaggtcga
      181 acgacttgag gatgcctatc tcgttcctga tgttctggcg cgggcggaag atatcgccgc
      241 cggggatcac cttgtccatc tcggcgaagc cgccgccata ctcatcatca ctgataagca
      301 tggtgctctg gacagtgtag agctcctcgc cccagcggtt gacgccgtaa gcgatggcca
      361 gggtcaggaa gagcgctgcg gcaaaccagt accagttgct gatgaagagc gaaaaatacc
      421 tgcgaaagtc tatcccttca ctttccggta tgtagaagct ctgttcccgg ttttccattg
      481 ttatttgctt ttaattgttt ctgtcatcct tccgtcaggg tttaaagaat tacattttat
      541 acaactctcc tctcatcatc cttcaccgaa ccccgcagag cctgccccga cagcccgtgg
      601 ctaccggagg gttcaatgat tgtatgatgc agcaaaccca aaaaatacct ccctctctct
      661 tatttgaagt agtttatcag cagcaatgtt gtggatatgg tgctggtgac cgtggcaaga
      721 atgaacgaga ttgtcggcag gttgacgctg aagaccttct tcgggttggg ctgcactatc
      781 accacgtcgt tgggggtgat gaagtatgcg ggcgacgaga ggaggctttt gtcctgcagg
      841 tcgatgtgat atgtaagggt ctgttcgccc tgcgggcgga taacgaggat tttctccttc
      901 gtgcctgtct cggagatgcc tcccgcgcga ccaatggcct caagaactgt gagctgatcg
      961 ttgtagttga cgtagctgcc gggagccctc acctcaccta tgacggtgaa cttgaagctc
     1021 atcaggcgca cctccacata ggcatggcgg aagagcgagt cgatcttctg ctggaagaga
     1081 tcacgtatct cgtagatggt caagcctgcc accgggatgt ctccgagcaa cgggagagtg
     1141 acatagccct gcgggttgat gctgtagccc atcatgtacg aggtaccctc gtttgatata
     1201 tagttcatat tggtggaggc acggtcactg agcatctcgg tgagtgttcc ctcgggggtt
     1261 tgtgccttga cgctgacata gatgatgtca cgtggctgta ccttgtagtc agggacctcc
     1321 atggggaact ggtttgctcc gtcgacctct tcgaggttat tgaggtatga gagttccttc
     1381 tgggtagcgc agccggccat gaagaggagc agcgccaaca gtgggaacag cgtctttgca
     1441 tgtcttaggt ctgaaggtct gtatgtcata gttttctgac gggtatttgg cgtattaaat
     1501 atctgtattt tttccgattt tacgtatgga gttggtttta atatccacgg tgaggttcat
     1561 tccgggctgg atgactctta tcaccacctt gtgtttgcgg ccaacgcgta tcagttcacc
     1621 ccggaggccg gtaaggctgc cgacggtcac ttcaaccatg tcaccctttt cataaacctc
     1681 ggcactgatc tctacatcag cgtcgctgcc acagaggatg cgaaggttgg tgatctgctc
     1741 ctccgggatg ggaatcggct tgccctgtat catgatgaac ttgaccaccc cctctgtctt
     1801 gcgtacggtg aagtactcgc gctgaatgat tttaacgaaa acataagagc tgatgagcgg
     1861 tctctcaacc agcttcttgc ggtctgacca ctggcgtagt gtcttctgga gtgggaggaa
     1921 tgattcgatg ccctgttcga tgagccgttc atggacccgt ttctcagccc tggagttagt
     1981 atagatggca taccactgct tcacaggtct cctcagttcc ctgggcgcct gtgaagtggc
     2041 ggcctgtggt ggagtggcct gtggtggggc agaggccgat ggcttggcgg cctgtggcgg
     2101 agtggcttgt ggtggggtgg tctctggcgg agtggtgccc ggcgacgtgt cagcctgtgg
     2161 tggagtggcg cccggcatcg tgtcagccga ttgggagtca gctgacaacg gggcagcgcc
     2221 tgtcaacggg gcagcctgtg gcggggcggc cgacaacgaa gccgtacccg acggcggagt
     2281 tacaaccgac tgtgactggg tctcttccat ggtccttttt aatacagaat gcaaaattaa
     2341 taaaattttg cggcaggtgt atggcatttc caatgccatg ccccgcctgc ccgccatcac
     2401 aatccctgaa gccctgcagg cactgatcag catgttgatg cttgtcagca acttattaat
     2461 aaatatgtgt cagaggaggt tttcaggctt catcaaacta cctgtaagca taattatatg
     2521 aaagctgcga ctgaataaat tcttcagaac ccccacacca gcccgccact tactgtggtg
     2581 gtgctgccat agagatgaga ggggtaccac tcttcggtgc cggtgacatc acgcatgatc
     2641 acggaggccc gtgcctgcac accgccggtg atcat
//
LOCUS       DAOPBK010000256         2671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_30143_length_2671_cov_6.433104, whole genome shotgun sequence.
ACCESSION   DAOPBK010000256 DAOPBK010000000
VERSION     DAOPBK010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30143_length_2671_cov_6.433104"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLA17_10890"
     CDS             <1..324
                     /locus_tag="PLA17_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPK85958.1"
                     /translation="GVEVVGIDEAQFFDNSLVEVCTMLADGGTRVIVAGLDMDFSGRP
                     FGPIPALMAVAEYVSKVHAICVRCGNLANHSYRKIKSDKVVVLGERDIYEPLCRSCYV
                     KAVNR"
     gene            321..>2671
                     /locus_tag="PLA17_10895"
     CDS             321..>2671
                     /locus_tag="PLA17_10895"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HPK85959.1"
                     /translation="MIIGARELAHITGGTLTGDENLKVTGIMTDSRSPGAGPDMLFIA
                     LRGPNHDGHKFIEPLYARGVRLFIADTLPPGHGEMAGAAFVKVTDTLDALQCIAKWRR
                     ESFRGRVIAVTGSAGKTIVKEWLAGVMSSSRTVIRSPKSYNSQVGVALSLMNLDESHE
                     IAVIEAGISRPGEMERLARIIRPDIVIITNVGDAHGENFSGREAIAAEKLRLASGAQL
                     LICNADDRIIRNEMASKYPEVPLFTWSLAGNAASLEVEAADTVDNGRILRCKTADESF
                     EAAIPFADHASAENAVSVIACCLAEKMPSELIAGAMAELPAVAMRMETKRGINGCTLI
                     EDYYNSDPASLGMALDFLQTRARGKTTLILSDFRQIGGDEKQLYAGVARAVRNSGIDR
                     FIGIGEALSRQRDSFQPGSLFFSSTADFADAIPGQQFRNETILIKGARVFGLERISRL
                     LVRQLHLTRLEINMNAVVHNLNEFRHLLKPGTGIIAMVKAFAYGSGAAEIASLLEYNG
                     VSRFAVAYADEGVALREAGITSPVMVMNPDESVMELIIHHDLEPEIYSIGFYSAFAEV
                     ARRHGLLHYPVHIKLDTGMHRLGFDMSDMEELTRQLAGQQHLKVVSLFSHLAASDDPE
                     HDAFTHRQARRLTDAAERLWKALGYRFSLHLLNTSGLVRFPEYQFDMVRLGIGLYGIG
                     HHEGVMLQNTGRFVTAVSQVRRVPAGDPVSYGCRGVSDHERQIAVIPVGYADGLRRQL
                     GDGRGALFIKGRRVPVVGSICMDMCMADVTGMDVRAGDEAEIFG"
BASE COUNT          630 a          636 c          816 g          589 t
ORIGIN      
        1 ggcgtggagg tggtggggat agacgaagcc cagttctttg acaacagcct cgtcgaggta
       61 tgtaccatgc ttgcagacgg cggcacgcgt gtgattgtgg caggcctcga catggatttc
      121 tcggggaggc cgttcgggcc catccctgcc ctgatggccg ttgccgagta tgtgagcaag
      181 gtgcacgcca tttgtgtcag gtgcggaaac cttgcaaacc actcctatcg taagataaag
      241 agcgataagg tggttgtgct gggcgaaagg gatatatatg agccgctgtg ccgctcatgt
      301 tacgtaaagg ctgtaaacag gtgataatcg gggcccggga actggcgcac atcacaggcg
      361 gaacgcttac aggtgatgaa aacctgaagg tcaccggcat aatgactgac agcaggtcgc
      421 cgggcgctgg ccctgacatg ctcttcatag ccctgcgggg acccaatcat gacggacata
      481 aattcataga acctctctat gcaaggggtg tgaggctgtt tattgctgac actcttcccc
      541 ccggacacgg tgagatggcc ggtgcagctt ttgtaaaggt gacagatacc ctggatgcgc
      601 tccagtgcat agcaaagtgg cgccgtgaga gcttcagggg cagagtcata gctgtaacgg
      661 gaagcgccgg aaagacgata gtcaaggagt ggctggcagg ggtgatgagc agttcccgaa
      721 ctgttatccg cagccctaaa agctacaact cacaggtagg cgtggcactc tcgctgatga
      781 atcttgatga gagccacgag attgccgtca tcgaagccgg gatctccaga cccggggaga
      841 tggagaggct ggcacgcatc atcaggccgg atattgtaat cattacaaat gtgggtgacg
      901 cccatggcga aaacttttcc ggccgggagg ccatcgcggc ggagaagctc agactggctt
      961 ccggggcaca acttctgata tgcaatgcag atgacaggat tatcaggaat gagatggcgt
     1021 caaagtatcc ggaggtccct ctctttacat ggtcactcgc aggtaatgct gccagccttg
     1081 aagttgaggc agccgatact gttgacaacg gccggatact gcggtgcaaa acagcagacg
     1141 aatcatttga ggcggccatt ccttttgctg atcatgcctc tgcagagaat gctgtctcag
     1201 tcatcgcatg ctgcctggct gaaaaaatgc catcagagct gatagccggg gcgatggcag
     1261 agctgccggc ggtggcaatg cgcatggaga cgaaacgggg cataaacggc tgcaccctga
     1321 tagaagatta ctataactct gatcctgcct cccttggaat ggcgctggat tttctacaga
     1381 cccgtgcaag gggaaagact accctcattc tgtctgactt cagacagata ggaggagatg
     1441 agaagcagct ttatgcgggt gtggccaggg ctgtaagaaa ctcggggatt gaccggttta
     1501 tagggattgg cgaggctctg tcacggcaga gggattcatt tcagccggga tcactcttct
     1561 tcagttcaac ggcagatttt gcagatgcca tccccggaca acagttcagg aacgagacaa
     1621 tcctgatcaa gggggcacgt gttttcggac ttgagcgtat cagcaggctt cttgtaaggc
     1681 agcttcacct cacacggctg gagataaata tgaatgccgt ggttcataac ctcaatgaat
     1741 tcaggcactt acttaaacct ggcaccggga ttattgcgat ggtaaaagca tttgcctacg
     1801 gctccggagc ggccgagata gcatctcttc tcgagtacaa cggggtgagc cgttttgcag
     1861 ttgcctatgc agatgaaggt gtggcactga gggaagccgg catcacgtcg cctgtcatgg
     1921 tcatgaatcc ggatgaaagc gtgatggagc tgataattca tcatgacctc gagcctgaga
     1981 tatacagtat cgggttttac tcggcttttg ccgaagttgc cagacgacac ggccttctcc
     2041 attaccccgt gcatatcaag ctagacacgg ggatgcacag gcttggattt gacatgagtg
     2101 atatggagga gctgaccagg caacttgcag ggcagcagca tctgaaagtt gtttcactct
     2161 tctcacatct tgctgccagt gatgatccgg agcatgacgc atttacgcac cggcaggcca
     2221 gacgactgac agatgcggcc gagaggctgt ggaaagctct tggctaccgc ttcagcctcc
     2281 atctgcttaa cacttcagga ctggtccgct ttccggaata ccagtttgac atggtcaggc
     2341 ttggaatagg tctgtacggt attggtcatc atgagggggt aatgctacag aacaccggaa
     2401 ggtttgtgac tgccgtatca caggtaagga gggttcctgc cggcgatccg gtcagctatg
     2461 gctgcagggg cgtctcagac catgagaggc agatagcggt tataccggtg ggctacgccg
     2521 acgggctgcg ccggcaactg ggagacggaa gaggcgctct tttcattaag ggccggaggg
     2581 tcccggtggt tggcagtata tgcatggata tgtgcatggc cgatgtcacg ggaatggatg
     2641 taagagccgg agatgaggct gagattttcg g
//
LOCUS       DAOPBK010000257         2669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_30170_length_2669_cov_5.267406, whole genome shotgun sequence.
ACCESSION   DAOPBK010000257 DAOPBK010000000
VERSION     DAOPBK010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30170_length_2669_cov_5.267406"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLA17_10900"
     CDS             complement(<1..1027)
                     /locus_tag="PLA17_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85960.1"
                     /translation="MDFLEKVAQRLHAQHGSEMERQTVVLPSRRAGLWLARALAKLND
                     KPSWAPSMMTVSDLFRSFTDLVPADTETLIFELYNVFRKQFGGEMSFDDFWPWGEVII
                     TDFNDIDLYMADAEKLYRNISDLKEIDEKFGGLTDEQIEIIRGFWKSFNPASSGSEAR
                     SKFRSVWQKLGPLYNGYRGELKARGMAGDGMLCREVAEKAIAGKLNVPEGRRWHIVGL
                     NALNTCEKNLFLHLKRLGVADFYWDDDHFFMDDPEHKASLFIRENRRIFGSALEESPS
                     CRHEPPAGEWHIIDTPSDTAQAGMVAQLLENEGITGSDDLTSTAVILADEKLLMPVLG
                     SLPESVEE"
     gene            complement(1030..1989)
                     /locus_tag="PLA17_10905"
     CDS             complement(1030..1989)
                     /locus_tag="PLA17_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133901.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPK85961.1"
                     /translation="MNRETIYGWGTARRFNSFADWFTGLVGGRVQKLSINAGFTCPNR
                     DGTLGYGGCTYCNNEAFIPGYCTPDKSVSEQIEQGIAFHRLRYRRAKHYIAYFQAYTN
                     TYAGIERLTALYNEALNHPLVAGIVIGTRPDCISGELLDRLALMARDYFVMIEYGIES
                     VNNDVLRRINRGHDFATAVQALEETSLRGLYAGAHLIFGLPGESRRAIADSAEVISSL
                     PLASLKIRQLQLVKGTAMAREYEQNPGAFDLYSLDDYLDLVISFTERLSPGIMIDRIS
                     GEAPPRMLADPRDWHMRSNEIFGLFEKRLEERNTWQGRLCKKR"
     gene            complement(2043..>2669)
                     /locus_tag="PLA17_10910"
     CDS             complement(2043..>2669)
                     /locus_tag="PLA17_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85962.1"
                     /translation="NLPAGFITRDDEAVRVVECGELPPVERDERQPGLEMSRFTLSEP
                     RGRLRLRTGGALPADEVKLTEPGGRAYGIMMHEILSRITTTHDIEAAVEHACTAGLMP
                     VTSRDAAVTRLRTMLSAEKVRSWFDGSASVMTEATIILPTGAARRPDRVMISGDTVTV
                     IDYKFGEPLPRHKQQAADYRQLLLQMGYSDVRSWLWYVEKDIIEEADD"
BASE COUNT          558 a          792 c          662 g          657 t
ORIGIN      
        1 cctcttccac cgactccggc agggaaccaa gaacaggcat cagcagtttc tcgtcagcaa
       61 ggatcacggc ggtggatgtc aggtcgtcgc tgccggttat gccttcattt tcaagcagct
      121 gtgccaccat gccagcctgt gccgtatcag agggtgtatc aatgatatgc cattcaccgg
      181 cgggtggttc atgtctgcac gaagggctct cctccagggc gctgccgaag atacgcctgt
      241 tctccctgat gaaaagagat gccttgtgct cagggtcatc cataaaaaag tggtcatcat
      301 cccaatagaa atctgcaaca cccagacgtt tcagatgaag gaacaggttt ttttcgcagg
      361 tattcagggc gttgagacct actatgtgcc atctcctgcc ctccggcaca ttcagttttc
      421 ctgcgattgc cttctcagcc acctcgcggc aaagcattcc gtcaccggcc atgccgcggg
      481 ccttcaattc ccctctgtac ccgttataaa gcggaccgag tttctgccag acagacctga
      541 attttgagcg ggcttcgctg ccggaagagg caggattgaa agatttccag aaaccgcgta
      601 taatttcaat ctgttcatct gtaaggccgc cgaacttctc atctatctcc ttcagatcgg
      661 agatgtttct gtagagcttt tcggcatcag ccatataaag atcgatatca ttgaagtcgg
      721 ttataatcac ttctccccac ggccagaagt cgtcgaagct catctcccct ccgaactgtt
      781 tcctgaagac gttgtaaagt tcaaatatca gggtttccgt atctgcaggc acgaggtcag
      841 tgaatgaccg gaacaggtca ctgacagtca tcattgacgg ggcccacgac ggtttgtcat
      901 tcagctttgc cagggcacgt gccagccata gtcctgccct ccggctgggg agtaccaccg
      961 tctgcctctc catctcgctg ccgtgctgtg catggagcct ctgtgccact ttctccagga
     1021 aatccattct cacctctttt tgcagagcct cccctgccat gtattccgtt cttcaagccg
     1081 tttttcgaac agcccgaata tttcattgct tctcatgtgc cagtcacgcg gatcagcgag
     1141 catacgcggg ggcgcttctc cgctgatgcg gtcaatcatt ataccgggcg agagccgctc
     1201 cgtgaaggag atcaccaggt caagatagtc gtcaagtgaa taaaggtcaa acgcgccggg
     1261 attctgctca tattcacggg ccatcgctgt tcccttcacc aattgcagct gccttatctt
     1321 gagcgatgca agtggcaatg atgagatgac ctccgcactg tcagcgatgg cgcggcggct
     1381 ctcgccaggc agtccgaaga tgagatgcgc ccccgcgtaa agtcccctca gcgaagtctc
     1441 ctccagggct tgcactgccg tggcgaaatc atgcccccgg ttaatcctcc tgagcacatc
     1501 attattaacc gattcaatcc catattcaat catgacgaaa taatcacggg ccatcagcgc
     1561 cagcctgtca agcagctcac cgctgatgca gtcaggacgg gtacctatga caatgccagc
     1621 caccaagggg tggttcagcg cctcattata aagggcagtc agtctctcaa tacctgcgta
     1681 tgtatttgta tatgcctgga aatatgcaat atagtgtttt gcacgacggt atctcaggcg
     1741 gtgaaaggct atcccctgct ctatctgttc cgacacactt ttatcagggg tgcagtaacc
     1801 aggtatgaag gcctcgttat tgcaataggt gcatccaccg taaccaaggg ttccgtcacg
     1861 gttggggcat gtgaatcctg cgtttatgct cagcttctgc acccgtcctc cgacaagtcc
     1921 ggtaaaccag tcagcaaagg agttgaatct tctggccgtt ccccaaccat atattgtttc
     1981 tctgttcatt cctcctctat tgcatcctat cccttttccc gtctgccctt catccccgga
     2041 agctaatcat ctgcctcctc tattatgtcc ttttcaacat accacagcca cgacctcaca
     2101 tcactgtatc ccatctgcag aagtagctgg cggtagtcgg cagcctgctg tttgtgtcgt
     2161 ggcaacggct ctccgaactt atagtcgatt accgtcactg tgtcaccgct tatcatcacc
     2221 ctgtcaggtc ggcgggcggc gcccgtgggg agtatgattg tggcctcggt catgactgac
     2281 gcgctgccgt cgaaccagga gcgcaccttc tctgccgaga gcatggtgcg gaggcgggtt
     2341 acagccgcat cacggctggt caccggcatc agcccggcgg tgcaggcgtg ttccacggct
     2401 gcctcaatgt catgggtggt ggtgatgcgg ctgagaatct catgcatcat tatgccgtat
     2461 gccctcccac cgggttcagt gagcttgacc tcatcggcgg ggagggcgcc gcccgtcctc
     2521 agtctgagtc tgccccgcgg ctcgctcagg gtaaaccgtg acatctccag ccctggctga
     2581 cgctcatctc tctccacagg tggcagttca ccacattcga caaccctgac agcctcatcg
     2641 tcgcgcgtaa tgaagccggc cggaagatt
//
LOCUS       DAOPBK010000258         2668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_30193_length_2668_cov_2.897436, whole genome shotgun sequence.
ACCESSION   DAOPBK010000258 DAOPBK010000000
VERSION     DAOPBK010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30193_length_2668_cov_2.897436"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1822
                     /locus_tag="PLA17_10915"
     CDS             227..1822
                     /locus_tag="PLA17_10915"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPK85963.1"
                     /translation="MKKTGIISHLFMIMAVACLLSSCKNETVITFRSLLREMACAETL
                     TRYPSPDYRLVQFSSYDRSSIHPDSAGWYANNDYTHFIREETANGRREYVMLEADGPG
                     AIVRWWMTFGNEDALNSYIRVYLDGQAEPVIEGMAPELVGGGVLAPEPLSTSVSPLTE
                     PQRRGYNLYLPIPFAEKCIITLENDSLLITPQRRKPSIYYNINVRLYDEGTRVVSAKS
                     ETFRDDTLAIRECAANLSKSNSSQATGKEQSSSGGVFPPGTECQISVTGKNLAVSNIS
                     LRLSGPDTAALLKGAVIRMWFDGHRTVEVPAGNFFGTGYTFNPCRTRFTAADTDGRLS
                     SSWLMPFRDSCRISILNNSNDTIGVETLVTMAPYRWDESSMYFGASWKEYHGFETAGS
                     ENTGGTGLHTDLNIARLNGKGLYAGDAVLVFNTADAWWGEGDEKIYTDGEAFPSSFGT
                     GTEDYYGYAWCRPEPFNHPLIAQPAGNGNFHPGMSVNMRYRILDAIPFRESLRADIEM
                     WHWAKTTIDFSVTAWFYMLPELK"
     gene            complement(1869..2447)
                     /locus_tag="PLA17_10920"
     CDS             complement(1869..2447)
                     /locus_tag="PLA17_10920"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HPK85964.1"
                     /translation="MALTNGTKGNVGRVKLIRRIHRLTASFLSVVLVIVAISGILLGW
                     KKNSSGYLHPVSHEGTTADLRQWLPLDSLNAIAIRTLHETGRKGLSEELDRIDIRPDK
                     GMVKFVFARHYHGIQLDGATGKVLYVENRRSDLVEDIHDMSVIDRLLGIDGEVFKLVY
                     TTFTGLLLLVFTVTGLWLRYFPRNGKGKDDKL"
BASE COUNT          688 a          727 c          650 g          603 t
ORIGIN      
        1 gatggcagcg ccggtcagca ggcagcggta ccggttggca gtactgatca acagtaccag
       61 catcatcagc aggcaggcgg atgtaggcaa cactggtcag cagtaccaat ctgctgcacc
      121 ggctggcatc acatactgca ttgagcatca ccaatgtcac atcagtgaat attatccaac
      181 aagaattgta aaagaatgga taactttatt cccaaaccac tgaccaatga agaaaaccgg
      241 aatcatcagc cacctgttca tgattatggc tgtcgcctgc ctgctctctt cctgtaaaaa
      301 tgaaaccgtg atcaccttcc ggtcgctgct ccgtgagatg gcctgcgcgg aaacgctgac
      361 ccgataccct tcgccagact acaggctggt gcagttcagc tcgtacgaca gaagcagtat
      421 ccatcctgac agcgcaggat ggtacgccaa taatgattac actcatttca tccgtgagga
      481 gaccgccaac ggacggcgtg aatatgtcat gctcgaagca gacggccccg gcgctatcgt
      541 ccgctggtgg atgacattcg gaaatgaaga tgccctgaac tcatacatca gggtttatct
      601 cgacggccag gccgaacctg taattgaagg catggctccg gaactggttg gcggaggtgt
      661 tctggccccc gaaccactca gcacatcagt ctcaccgctg acagagccgc agcgccgcgg
      721 atataacctc tacctcccca taccgtttgc tgaaaagtgc atcatcaccc ttgaaaacga
      781 ctctctgctc ataactccac agagacgcaa accctcgatt tattacaaca ttaatgtccg
      841 gctgtatgac gaaggcacca gggttgtctc tgcaaaaagc gaaacgttcc gcgatgacac
      901 ccttgccatc agagaatgtg ctgcaaatct cagcaagtcg aacagcagcc aggcaaccgg
      961 taaagagcag agttcatccg gcggggtgtt ccctcccggc acggaatgcc agatatcagt
     1021 gacaggtaaa aaccttgctg tcagcaatat ctcactcagg ctctccggac ctgatactgc
     1081 agccctgctg aaaggagctg tcatccggat gtggtttgat ggccacagga ccgtggaggt
     1141 gccggcgggt aacttcttcg gcaccggtta cacattcaac ccctgcagaa cccgtttcac
     1201 agctgcggat actgacggca ggctgagctc ttcatggctg atgccattcc gcgacagttg
     1261 caggataagc atcctcaaca acagcaatga cacaattggc gtggaaacgc ttgttacaat
     1321 ggcgccctac aggtgggatg aaagctcaat gtatttcggg gcctcctgga aggagtacca
     1381 tgggtttgaa actgccggct cagagaacac cggaggaacg ggtctgcata ctgatctgaa
     1441 tatagccagg ctgaatggaa aaggtctata tgccggtgac gcggtgctgg ttttcaacac
     1501 tgctgacgcc tggtggggcg agggtgatga gaagatttac actgacggcg aagctttccc
     1561 ctcatccttc ggaacaggaa cagaggatta ttacgggtac gcatggtgcc ggccggaacc
     1621 gttcaaccat ccgctgattg cacaaccggc cgggaatggc aactttcacc cggggatgag
     1681 tgtgaatatg cgctacagga tcctggatgc gataccattc agggaatcac tccgggccga
     1741 tattgaaatg tggcactggg caaagacaac catcgacttt tcagtcacag cctggttcta
     1801 catgttgcct gaattgaaat aagtgcaccg gccagggccg ctcatgctgt cctgccaggg
     1861 aagccggact atagcttatc atctttcccc ttcccgttcc ggggaaagta gcggagccat
     1921 aagccggtaa cagtgaacac cagcaataac aggccggtaa aggtggtata gacaagcttg
     1981 aacacctcac catcaatgcc aagcaacctg tctattactg acatgtcatg aatgtcttca
     2041 accaggtctg acctcctgtt ctcgacataa agcaccttcc ccgtagcacc gtcaagctga
     2101 atgccatggt agtggcgtgc gaacacaaac tttaccatcc ctttgtcagg ccttatgtcg
     2161 atcctgtcaa gctcctcgct gagtcccttc ctgcctgttt catgaagcgt ccttatagct
     2221 atcgcattaa gcgagtcaag cggaagccat tgccgcaaat cggcagtagt cccctcatgg
     2281 gagaccggat gcagataccc gctgctgttt tttttccagc cgaggagtat ccccgaaatg
     2341 gcaactatta ccaggacaac agacaggaat gaggctgtca ggcggtgaat tctcctgatc
     2401 agtttcaccc ttcccacatt tccttttgtg ccatttgtca atgccatact gcctgttttt
     2461 atttcagtgg aacaaaaaaa gtactatcga tctgaaccaa acgacctgct tccgggctac
     2521 ttctatgaaa aaaccagatt gtcaccataa tcagttatta caacaaacta ataccacctc
     2581 tgtcgcctca tcttgtcctt ctcattgaca ggatttgcat caagaacgaa cattccacgt
     2641 ccgtgagtgg atatgataat catattgt
//
LOCUS       DAOPBK010000259         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_30655_length_2636_cov_3.427741, whole genome shotgun sequence.
ACCESSION   DAOPBK010000259 DAOPBK010000000
VERSION     DAOPBK010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30655_length_2636_cov_3.427741"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..591)
                     /locus_tag="PLA17_10925"
     CDS             complement(4..591)
                     /locus_tag="PLA17_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPK85965.1"
                     /translation="MAHYPDIFSINPEDKIPGKGRVLISEPFLTDRFFNRTIVFLTEH
                     TAEGTVGFILNRVIDLMVSSAVDGFDGWDEHLSVGGPVAPDTLHYLHTLGQKVPGSVP
                     VMPGIWWGGEIDTLRNLIATGTVGRDQVRFFLGYSGWGKGQLKRELSENSWVIARVPP
                     EVIMTIRGNVWRDVLRSFNNKYRIWAEFPESPEMN"
     gene            724..2205
                     /gene="nhaC"
                     /locus_tag="PLA17_10930"
     CDS             724..2205
                     /gene="nhaC"
                     /locus_tag="PLA17_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HPK85966.1"
                     /translation="MLQSLLPIAVLILFLTLNVKFFHDDTLSGANQMALILASAVAGV
                     ISVSLGYKWLSIRAKVVKGIGTAMPAILILFLIGSLAGTWMVSGVVPMLIYYGLEILH
                     PKIFLFAAVVICALVSVSTGSSWSTVATIGVALLGIGKALGFNDAMIAGAIISGAYFG
                     DKMSPLSDTTNLAPAVAGTDLFTHVRYMVHTTVPSLILTMVIFLIIGFTIDLQGVTTS
                     ADAVQAAIKAKFNVTPVLLLVPAMLIYIIAKKVPPLPSLLAGTLLAALLAVIYQPHIL
                     KEVAATGGSFTKQAYIAIMKTMFGTVSVTTDNQAVNELLITRGMAGMLNTVWLIICAM
                     VFGGIMEASGMLKTITGKLMKFVHNAGTLVGSTVATCLFFNITASDQYLSIVIPGRMY
                     AKKFRDMGFKPELLSRTLEDSGTVTSVLVPWNTCGATQSSVLGVSTWAYAPYCFFNII
                     SPFMSVFIASIGYKIRRYTPEEHARVLEEADAADRELYQEEEA"
     gene            complement(2275..>2636)
                     /locus_tag="PLA17_10935"
     CDS             complement(2275..>2636)
                     /locus_tag="PLA17_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85967.1"
                     /translation="ALLPVHQCPAASSSVPRGQFIGTLRPIHQCPAANSSVPHGHLNA
                     IIYGRQPSPLLLVQLTAGAAPGVNRIAACIGMPGAGDTSLPHLLVVTHMGVRELPSRF
                     EKYGYSKTCQRKADQYG"
BASE COUNT          648 a          695 c          654 g          639 t
ORIGIN      
        1 atatcagttc atctccggag attcaggaaa ctctgcccag atacggtatt tgttgttgaa
       61 tgaacggagt acgtcacgcc acacgttacc cctgatcgtc ataatcacct cgggaggcac
      121 cctggctatc acccaggaat tctcactcag ctcacgtttc agctgtcctt tgccccaccc
      181 tgaatagccc aggaagaacc tcacctggtc acgtcctacc gtgcctgtag ctataaggtt
      241 acggagggta tctatctcgc caccccacca tatcccgggc atcaccggca cgctgccggg
      301 caccttttgg ccgagggtat gaaggtaatg aagggtatcc ggtgccacgg gcccccctac
      361 agagaggtgt tcatcccatc cgtcgaaacc atccaccgca ctgctgacca tcaggtcaat
      421 caccctgttg aggatgaatc ctaccgtgcc ttcagccgtg tgttccgtga gaaaaactat
      481 ggtacggttg aaaaaacggt cggtcaggaa gggttcagag ataagcactc tgcccttccc
      541 cggaatctta tcctcggggt tgatactgaa aatatcagga taatgtgcca tagaacacaa
      601 ataatacaaa gacaaaataa tatcttttga cggcaaaatc aaggaaaata gaggaaaata
      661 gtaaattgca cacttcaaca ccaggaacca tacatgtctt ccaaacagta caaaacaccg
      721 tcaatgctgc agtctttgct gcccattgcc gtgctgatac tcttccttac cctcaacgta
      781 aagttttttc atgatgacac tctctcggga gcaaaccaga tggctctgat tcttgcttcc
      841 gcagttgccg gagttatttc agtaagtctc ggatacaagt ggttgtctat ccgtgccaag
      901 gttgtcaagg gaataggcac tgccatgcct gccatcctga tcctctttct gatcggttcc
      961 cttgccggaa cctggatggt aagcggggta gtgccgatgc tcatttacta cggcctcgag
     1021 atactccatc ccaagatatt cctgtttgct gcggtggtca tctgtgctct tgtgtctgtc
     1081 agtaccggca gctcatggtc aacagtggcc accatcggcg tggcactgct gggtatagga
     1141 aaggctctgg gattcaatga tgcaatgatt gccggtgcaa tcatatcagg tgcatatttt
     1201 ggtgacaaga tgtcacccct ctcagatacc accaaccttg caccggcagt tgcaggtacg
     1261 gatctcttca cacacgtaag gtacatggtc cataccacag taccgtcact gattctcacg
     1321 atggttattt tcctgataat aggattcacc attgacctcc agggagtcac caccagtgct
     1381 gatgccgtac aggctgcaat aaaggcgaag ttcaacgtga cgcctgtcct gctgctggta
     1441 ccggccatgc ttatttacat catcgccaag aaggtaccgc cgctaccgtc gcttcttgcc
     1501 ggtacacttc ttgccgccct gcttgcagtc atctaccagc cgcacatcct taaggaggta
     1561 gctgctaccg gaggttcatt caccaagcag gcgtatatag ccatcatgaa gacaatgttc
     1621 ggtacagttt cagtgaccac tgacaatcaa gcggtgaatg aactgttgat tacacgcgga
     1681 atggccggca tgctcaatac cgtctggctc atcatctgtg caatggtctt cggaggaatc
     1741 atggaggcca gcggcatgct caagactatc acaggaaaac tgatgaaatt cgtacacaac
     1801 gcggggaccc tcgttggttc aacagttgca acatgcctct tcttcaatat taccgcatct
     1861 gaccagtacc tgtcaatagt tatccccggc agaatgtatg caaagaagtt ccgcgacatg
     1921 ggtttcaagc cggagctcct gagccggaca ctggaagact ccggtacggt tacctctgtt
     1981 ctggtaccgt ggaatacctg cggcgccaca cagtcgagtg tgctgggcgt ctccacatgg
     2041 gcatacgcac cctactgctt cttcaatatc ataagtccgt tcatgagtgt cttcattgcc
     2101 tcaatcggat ataagataag gcgctacaca cctgaggagc atgccagggt gcttgaggag
     2161 gccgatgctg cagaccggga gctttaccag gaggaggagg cctgagggct tgtgatttaa
     2221 ttgtaaattt gcattgcaga gagaaatatt atgctgttaa agctttttat aatatcagcc
     2281 atattgatcg gccttgcgct ggcaggtctt gctatatcca tacttctcaa accgggaggg
     2341 cagttccctg acacccatgt gggtcacaac aaggagatgc ggaagagagg tatcacctgc
     2401 gcccggcata ccgatacagg ctgcaatcct gttgacacca ggggctgctc cggctgtgag
     2461 ttgaacaagg agtagtggtg atggctgcct gccataaata attgcattca aatggccatg
     2521 tggtactgat gaattggccg cagggcactg atgaattggc cgcagggtac cgatgaactg
     2581 gccgcggggt accgatgaac tggccgcggg gcactgatga actggcagca gagcac
//
LOCUS       DAOPBK010000260         2617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_30940_length_2617_cov_3.678767, whole genome shotgun sequence.
ACCESSION   DAOPBK010000260 DAOPBK010000000
VERSION     DAOPBK010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30940_length_2617_cov_3.678767"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="PLA17_10940"
     CDS             <1..1922
                     /locus_tag="PLA17_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPK85968.1"
                     /translation="AARNNWSAAVPKLDENGERVYVDSSTGQVVPSGTPGAIPAYQLS
                     MWYTAPGGNPDHIYRRAMNEERNTLNLYSTYALNVLDDHQFNFMLGMNRVGYDEAYNW
                     SQTTQLIDYSNPQFDLAYGTMTNSGGEYWESQLGFYGRLNYNYKDKYLLEGNLRYDGT
                     SKFPTHLQWRWFPSFSAGWIVTEEPFMEWSKPILSMFKLRGSWGTIGDQTVSNSLYIP
                     TMGGSQSNWLLGGAKLYQFATPGAVSADVTWQDITTLNLGFDARLLKSRLGITLDWFI
                     RDTENMIVPQEGLPATFGTGAPQGNFGSLQTKGFEIQVDYNHRFQNGLGINLTATLSD
                     AVTKITKYGSTQSIDSWYVGKTYGEIWGYETDRLFQKEDFVYDSEGNLIKVTVPNEAG
                     SANYTIYQLADPANVAIQGRLQAGNFLFMPGDVKFVDLNGDGYINPGNRLIKGADGKP
                     DHGDLKIIGNSTPRYMYGFRADLDYKGFDLSFFIQGVGSRQVWGSGFLAIPGFHASDG
                     ATPKAFATDFWREDRTDAFYPRPYNLAGSSTTLNMVPQTRYLLNMSYLRIKNITFGYT
                     LPSTITEKIMLSRVRVYAALENFFTFDHLGTLPIDPEEVQGYSMWNSSYYNMGRTGVG
                     VPTFKSASVGLQVNF"
     gene            1934..>2617
                     /locus_tag="PLA17_10945"
     CDS             1934..>2617
                     /locus_tag="PLA17_10945"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK85969.1"
                     /translation="MKGKLTIIIALAALFFSGCEDFLDRPSLTTMNDQNYWTSENNLR
                     LFANGFYANYFVGYNSAWGVDYAPLRGYYFSDDFTQTGKQISFETQAPASRESTSETP
                     SMLSTYCGPSWNFAYVRKSNLFLERIDAMKDVYLTEEAYKHWSAVARFFRGYEYSRLV
                     SVFGDVPYYDRVVMDTELDLLYKDRDDRTMVMDAVYDDFAYVMANIRTSDLNPQYLNR
                     YIAAGFISRF"
BASE COUNT          679 a          620 c          641 g          677 t
ORIGIN      
        1 ttgcggcgcg taataactgg tcagctgcag tgcctaaact tgacgagaat ggtgaaaggg
       61 tatatgtcga ctcaagtaca ggacaggtgg ttccttcagg tacccccggc gccatcccgg
      121 catatcagct gtcaatgtgg tatacggctc ccggcggaaa ccctgaccat atatacaggc
      181 gcgctatgaa cgaagagaga aacacattga acctttactc gacatatgca cttaatgtcc
      241 tggatgacca tcagttcaat tttatgcttg ggatgaacag ggtgggatac gatgaggctt
      301 acaactggtc tcagaccaca cagcttattg attattccaa tccgcaattt gacctcgcat
      361 acggaaccat gaccaacagc ggaggagaat actgggaatc tcagcttgga ttttatggcc
      421 gcctgaatta taactataaa gataaatacc tgctggaggg aaatctcagg tatgacggca
      481 catccaaatt cccgactcat ctgcagtggc gctggttccc gtcattctca gccggatgga
      541 tagtgacaga ggaaccgttt atggaatggt caaagcccat cctcagcatg tttaagcttc
      601 gcggatcatg gggtactatc ggagaccaga cggtgtcaaa ctcactttat attcccacca
      661 tgggaggatc acagagtaac tggcttcttg gtggagctaa actttaccag tttgccaccc
      721 cgggtgccgt ttcagctgat gtgacatggc aggacattac caccctgaac ctgggattcg
      781 atgcacgtct gctcaagagc cggcttggga tcactctcga ctggttcatc cgtgatacgg
      841 agaacatgat agttccccag gaagggctgc ctgctacatt tggtacagga gcacctcagg
      901 gcaacttcgg atcacttcag acaaagggct tcgagataca ggttgattac aatcaccgtt
      961 ttcagaacgg tctgggcatc aatcttactg caacactttc tgatgctgtt accaaaatca
     1021 ccaaatacgg ctccacccag agcatagatt catggtatgt cggtaagaca tatggtgaga
     1081 tctggggcta tgaaaccgac aggctcttcc agaaggaaga cttcgtttat gacagcgaag
     1141 gcaatctgat aaaagttacg gtacccaacg aggccggtag cgccaactat acaatctatc
     1201 agcttgctga ccctgcaaac gtagccatcc agggaaggct tcaggcaggg aatttcctct
     1261 tcatgccggg agatgtcaag tttgtcgatt tgaatggtga cggatatatt aatcccggta
     1321 acaggctgat caagggtgct gacggtaagc ctgatcatgg tgatcttaag atcatcggca
     1381 attccacgcc gcgttacatg tatggtttcc gtgcagacct tgactataag ggatttgacc
     1441 tttcgttctt cattcagggc gtaggcagca ggcaggtctg gggtagtggt tttctcgcca
     1501 ttccgggatt ccacgcatct gacggagcca cgcctaaggc ttttgccact gatttctgga
     1561 gggaagacag gactgatgca ttttatcctc gtccctacaa ccttgccgga agcagcacaa
     1621 cactcaatat ggtgcctcag acaaggtacc tgcttaacat gtcatacctg agaattaaga
     1681 atatcacatt cggctacacg cttccttcaa ctattactga gaagatcatg ctcagcagag
     1741 tgcgtgtcta tgcagccctc gagaatttct tcacgtttga tcatctcgga acacttccca
     1801 ttgatccgga agaggtacag ggttattcga tgtggaacag ctcctactat aacatgggtc
     1861 gtacaggtgt tggtgtgcct actttcaaga gtgcatcagt tggtcttcag gttaacttct
     1921 aaaactagaa acaatgaaag gaaaattgac aattattatc gctttggcag cattgttttt
     1981 cagcggatgt gaagatttcc tcgacaggcc gtcactgaca acaatgaatg accagaacta
     2041 ctggacatcg gagaataacc tccggctctt cgccaatggg ttctatgcaa actacttcgt
     2101 tggctacaac tctgcatggg gtgttgacta tgctccgctt cgcgggtatt atttttccga
     2161 tgacttcact cagacaggca aacagataag ctttgagaca caggctcctg catctagaga
     2221 atctaccagt gagactcctt cgatgctttc aacctattgc gggccttcat ggaactttgc
     2281 gtacgtccgc aagtcaaacc tcttcctcga gagaatagat gcgatgaagg atgtgtacct
     2341 cactgaggaa gcatacaagc actggagtgc tgttgcccgg ttcttcaggg gttatgagta
     2401 tagcaggctc gtgagtgtct tcggtgatgt accatattat gacagggtgg taatggacac
     2461 tgaactggat ctgctctata aggacaggga tgaccgcact atggtaatgg atgccgtata
     2521 tgatgacttt gcatatgtca tggccaatat tcgcacttct gacctgaatc cccagtacct
     2581 gaacaggtac attgctgccg gatttatttc acgtttc
//
LOCUS       DAOPBK010000261         2612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_31008_length_2612_cov_4.134533, whole genome shotgun sequence.
ACCESSION   DAOPBK010000261 DAOPBK010000000
VERSION     DAOPBK010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31008_length_2612_cov_4.134533"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="PLA17_10950"
     CDS             <1..1582
                     /locus_tag="PLA17_10950"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK85970.1"
                     /translation="QGVRAVDPLISWILKYDNDEGHRAAATALTTILDSTGMRKIAAS
                     LPGSKGNSTVTLIRLLAWSGEKSYFREVLPLTGSDDIAIRAAAISALQALASYDDQKE
                     IIALLDATSDRAEVTELQRALLNAAMQGDTPAIRSALILQALDSSRDRLKLIPLLAGT
                     GGEEALKRVAAEFENGDAITRDVCFDALSHWSDHSAAGALISITASGNKTFGRPAFDA
                     YMRMVSVAPLPPERKLLMIKEIAPYAVAPDARAGMVAFAASLGVRQVEFFIAPYLSDP
                     AEEVRNAALEALESLKLPEDEEIEQKFNGKDLTGWQDLVDNPLTSAGMIPKELAAKEK
                     EAGFVALFNGKNLDGWIGNKVSYVAEEGMIVIRPGDGSGGNLYTEKEYSDFVFRFEFQ
                     LTPGANNGLGIRTPLEGDAAYVGMELQILDNTASIYANLKPYQYHGSVYGVIPARRGF
                     LRPVGEWNYQEVTVQGTKVKVVLNGTVIVDGDIAGAIANGTMDQKEHPGLSNSRGHIG
                     FLGHGSVVKFRNIRIREL"
     gene            1760..2122
                     /locus_tag="PLA17_10955"
     CDS             1760..2122
                     /locus_tag="PLA17_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPK85971.1"
                     /translation="MRILICEDNLMVSKAMSVVLEREGYDPVTVNDGNRAIEALQRND
                     YDLAIVDIHMPYHSGLEVIRYLRTELKKQTPVLIVTAFSDPQIRRQAGEMKVSGYLTK
                     PFDPDDLLKKIRAAVAVV"
     gene            2126..>2612
                     /locus_tag="PLA17_10960"
     CDS             2126..>2612
                     /locus_tag="PLA17_10960"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK85972.1"
                     /translation="MGHKSYIKVFTLMVILCMTAGLLWSQTVTDPEKEYARIRTLAFE
                     GKYDEAEPAARLLVKQFPEYGDARILLGRIIAWEGRYDEAAAVIDTLLASEPENSDAL
                     SALADIRRWSRDRSQQVTAPTDIRAGYMFDTFSEPYERFWQVFSLGAGHRFSRGTVVA
                     TV"
BASE COUNT          658 a          615 c          738 g          601 t
ORIGIN      
        1 gcagggtgtg agggcagttg atcctctcat ctcatggatt ctgaaatatg acaacgatga
       61 aggtcaccgt gctgctgcca cagccctcac aaccatactt gacagcacag gcatgaggaa
      121 gatagccgct tcacttcccg gctcaaaggg taactcaacc gtcaccctca tcaggctgct
      181 tgcctggtca ggtgaaaaga gctacttcag agaggttctt ccgttgaccg gttctgatga
      241 cattgccatc agggcagcag ccatctcggc gctgcaggca ctggcttcat atgatgacca
      301 gaaggagata atagccctcc ttgatgcaac atcagacagg gcggaagtga ctgaacttca
      361 gcgggcactg cttaatgccg caatgcaggg agacactccc gcaatcaggt cggcactcat
      421 tctgcaggcg cttgacagca gtcgtgacag gctgaagctc ataccgctcc tggcaggtac
      481 cggaggtgaa gaggcgctga aaagagtggc tgcggagttt gagaatggcg atgccatcac
      541 ccgcgatgtc tgttttgatg ctctctctca ctggagtgac cactcggcag ccggagcact
      601 gatcagtatt acggcctcag ggaacaaaac ctttggaaga ccggcatttg acgcctatat
      661 gcggatggtc tcggttgcgc cgctgccacc tgagcgcaag ctcttgatga taaaggagat
      721 agctccctat gctgtggctc ctgatgccag agccgggatg gttgccttcg cagcatccct
      781 gggagtgagg caggttgaat tctttattgc tccatatctg tctgaccctg ctgaggaggt
      841 cagaaatgcg gcactggagg ctctggaatc actcaaactg cctgaagatg aagaaatcga
      901 acagaagttc aacggcaagg acctcaccgg gtggcaggac cttgtcgaca atcctttgac
      961 cagtgccggg atgattccga aggaactggc agcaaaagag aaggaggccg ggtttgttgc
     1021 attgttcaac ggaaagaatc ttgacggatg gattggcaac aaggtatcct atgtggcaga
     1081 agagggaatg atagtgatca ggccgggcga tggcagcggt ggaaacctct acaccgaaaa
     1141 ggagtattcc gatttcgtct tccgctttga gtttcagctc actcccgggg ccaataacgg
     1201 ccttggaata cgcactccgc ttgaaggtga cgctgcatac gtgggcatgg agttgcagat
     1261 actcgataat accgcttcta tatatgccaa tcttaaacca taccagtatc acgggtctgt
     1321 ttatggcgtt atcccggcac ggaggggatt tctgaggccc gtgggggaat ggaattacca
     1381 ggaggtgacc gtacagggaa cgaaggtgaa agtggtgctc aacggaacgg ttatcgttga
     1441 cggtgatatt gcaggtgcca tcgccaatgg caccatggac caaaaggaac atccggggct
     1501 cagtaacagc cgcggacata tcggatttct ggggcatggc tcggtggtaa aattcaggaa
     1561 tatccgtatc agggagctct aaaagaattt agtcacaccc tattgttgaa tagtcaacga
     1621 tttatcaaca gacatcaaat tgatggtgtc tgaattttga aagcaatata aaaagaatca
     1681 tttttgaccg cctttagaca aggaaggagg ctttgctgcg ttataacaac gggagcggag
     1741 gcggtgaatt gagtagatta tgagaatatt gatttgtgaa gataacctga tggtatcaaa
     1801 ggcaatgtct gttgtcctgg aaagggaggg gtatgatccg gttacggtaa atgacggaaa
     1861 cagggctatt gaagccctgc agaggaatga ctatgatctg gccatagtag acatacatat
     1921 gccttatcac agtggtcttg aggtgatcag gtacctccgg actgagctga agaagcagac
     1981 gccggtactt attgttaccg ccttcagcga tccgcagata cgtcgtcagg ccggcgagat
     2041 gaaggtgagt ggttatctca ccaaaccgtt tgatccggat gatctcctga aaaaaatcag
     2101 ggccgcagta gcagtagttt agaagatggg gcacaagagt tatatcaagg ttttcactct
     2161 catggtcata ctgtgcatga ctgccggtct gctctggtcg cagaccgtca ctgatcctga
     2221 aaaggagtat gccaggatac gcacccttgc atttgaaggc aaatatgacg aggctgagcc
     2281 tgctgccagg ctccttgtga aacaatttcc tgaatatggt gatgcccgta ttctcctggg
     2341 ccggataatt gcctgggagg gacggtatga tgaagctgct gccgtgattg acacccttct
     2401 tgcctccgaa ccggagaaca gtgacgccct gtcagccctg gctgacatac gcagatggtc
     2461 acgcgacagg tctcagcagg tgacagcccc tacagatatc agggcaggtt acatgtttga
     2521 caccttcagc gagccttatg aacgattctg gcaggtcttt tccctcggag ccggtcaccg
     2581 tttctcccgg ggcactgtcg tggccaccgt ta
//
LOCUS       DAOPBK010000262         2589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_31384_length_2589_cov_4.143252, whole genome shotgun sequence.
ACCESSION   DAOPBK010000262 DAOPBK010000000
VERSION     DAOPBK010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31384_length_2589_cov_4.143252"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..615
                     /locus_tag="PLA17_10965"
     CDS             445..615
                     /locus_tag="PLA17_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85973.1"
                     /translation="MKVRCRPGSSGPGKHSEREKAGAGTVEESRYQPGSGGPGKYGNE
                     GEGRCRQRKGGH"
     gene            771..1145
                     /gene="rpsL"
                     /locus_tag="PLA17_10970"
     CDS             771..1145
                     /gene="rpsL"
                     /locus_tag="PLA17_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPK85974.1"
                     /translation="MPTIQQLVRKGRKKLADKSKAPALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHLVRGAL
                     DTSGVDGRKQRRSKYGTKRPKK"
     gene            1165..1641
                     /gene="rpsG"
                     /locus_tag="PLA17_10975"
     CDS             1165..1641
                     /gene="rpsG"
                     /locus_tag="PLA17_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPK85975.1"
                     /translation="MRKAKPKKRILLPDPKFNDPYVTRFINNLMYDGKKTVAFRIFYD
                     SLDIVAEKMKNVEKSPLEIWKQALDNITPHVEVKSRRVGGATFQVPLEIRPDRKVSIS
                     MKNLILFARKRTGRSMAEKLAAEVMAAYNNEGGAYKKKEDTHRMAEANKAFAHFRF"
     gene            1675..>2589
                     /locus_tag="PLA17_10980"
     CDS             1675..>2589
                     /locus_tag="PLA17_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPK85976.1"
                     /translation="MNSDLKHTRNIGIMAHIDAGKTTTSERILYFTGRVHRIGEVDDG
                     NATMDWMVQEQERGITITSAATTTFWKYRDQLYKINLIDTPGHVDFTMEVERSLRVLD
                     GAVAVFCAVGGVEPQSETVWRQANKYNVPRIAFVNKMDRSGADFFGVVQQMKDKLGAH
                     PVPVQIPVGSEENFDGIIDLVEMNAIMFEESDDPLMNFTIKPIPDFMKEEATEWRMKL
                     VEACAEMDDSILERYLEDHESITADELIAVLRRRTVEGVIVPVFCGAAFRNKGIQRLL
                     NGVATYLPSPIDVGAINGRDPRDDREVLR"
BASE COUNT          741 a          576 c          735 g          537 t
ORIGIN      
        1 ctcgataacc ccgccgttga aaagcattgc ttccgcgagg gtggtcttac ctgagccaga
       61 gtttcccagc agagcaatgt ttcttaggtg gtctgttgag tatgtcttca tcttaggctg
      121 ttgttatatt atataatgtt aatctgtacg gaatggagcg caaaaataat aaattttcaa
      181 aagagtgcat ataaagtgat ggttgtttga aaaatattta cttatctttg cggcggtttt
      241 ttgaaaacag gagtaccggt ctctccgtca gtgtgtaagt ttacaaaaga tgtcgggagg
      301 agcggtcggg aagaaaatcc tggaaatgac ttacaaacca taagttacat cttcagaaaa
      361 cttgaccccg ggtatgccgt ggcggctgtt tatcgtcata gtggcccgga tgtacagaat
      421 gtactgacgc ggtatcagcg ccgaatgaag gtcaggtgcc ggccaggcag cagcggcccc
      481 ggaaagcaca gtgagaggga gaaggcaggt gccggcacgg tagaagaaag cagataccag
      541 ccaggcagcg gcggccccgg aaagtatggg aatgagggag agggcaggtg ccggcagcgt
      601 aagggaggcc actgaaggta cgggaagagg gagaaggcag gtgccggcag cgtaaaggag
      661 accactgaag gtatgggaag agggagaagg caggtcggta caaagaggag agggaaagaa
      721 tagtattatt taatgaccag aatataaaag aaaattaatt aagaaagagt atgccaacaa
      781 ttcagcagtt agtaagaaaa ggccgcaaga agctggcaga caagagtaag gcacctgctc
      841 ttgactcatg cccgcagcgc aggggagtat gtgttcgcgt atatactacc acgcccaaga
      901 aacctaactc agccatgaga aaagttgcca gggtaaggct gaccaaccag aaagaggtca
      961 atgcctacat cccgggtgaa ggacacaacc tgcaggagca ctccatagtg cttatcaggg
     1021 gcggaagggt aaaagacctc ccgggtgtcc gctaccacct tgtccgtggc gcacttgata
     1081 catccggtgt tgatggccgc aagcagagaa ggtcaaaata cggaacaaaa agaccaaaga
     1141 agtaacagtt aaaggattca agatatgaga aaggcgaaac caaagaaaag gatcctccta
     1201 ccggacccga aattcaacga tccgtacgtt acccgtttca tcaataacct gatgtacgat
     1261 ggtaaaaaaa ccgttgcctt caggatattt tatgactcac tcgatattgt cgccgagaag
     1321 atgaaaaacg tggagaagag ccccctggag atttggaaac aggctcttga taatatcact
     1381 ccgcacgttg aggttaaaag ccgcagggtt ggtggtgcca ccttccaggt gcccctcgag
     1441 atacgtcctg accgcaaggt gagcatcagc atgaagaacc ttattctctt cgcgcgtaag
     1501 cgcacaggcc gcagcatggc cgagaaactt gcagcagagg tgatggcagc ctacaacaat
     1561 gaaggcggcg catacaagaa aaaagaggat acccaccgta tggccgaagc caacaaggca
     1621 ttcgcccatt tcaggttctg ataacctgta agtgtggtta gccgggagca gagaatgaac
     1681 agcgatctca aacataccag gaacattggc ataatggccc atattgatgc cgggaagact
     1741 acaacttccg agcgcattct ctatttcacg ggccgtgtac accgcattgg cgaagtcgat
     1801 gacggcaatg ccaccatgga ctggatggtg caggagcagg agagagggat cactatcaca
     1861 tcagctgcca ctaccacatt ctggaaatac cgggatcagc tctacaagat caacctcatt
     1921 gacacgccgg ggcacgtaga cttcaccatg gaggttgaga ggtctctgcg cgtacttgac
     1981 ggtgcagtgg cggtcttctg cgctgttggc ggcgttgaac cccagagcga gaccgtctgg
     2041 aggcaggcca ataaatacaa tgttcctcga atagcgtttg tcaacaagat ggaccgctcc
     2101 ggtgctgatt tcttcggagt ggtgcagcag atgaaggaca agctgggggc tcacccggtg
     2161 cctgtgcaga tacctgtcgg ttccgaggaa aatttcgatg gcatcattga ccttgttgag
     2221 atgaatgcca ttatgttcga ggagagtgat gacccgctca tgaacttcac tatcaagcca
     2281 atacctgact ttatgaagga ggaggcaacc gagtggagga tgaagctggt tgaggcatgc
     2341 gccgaaatgg atgattctat ccttgaacgt taccttgaag atcatgagag cattactgct
     2401 gatgaactta ttgcagtact gcgccgcaga accgttgagg gagtaatagt tcccgtcttc
     2461 tgcggagccg ctttcaggaa caaggggata cagcgcctgc tgaacggtgt ggcaacatac
     2521 ctgccctcac cgattgatgt gggtgccatc aatggaagag acccccgcga tgacagggag
     2581 gttttgcgc
//
LOCUS       DAOPBK010000263         2495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_32959_length_2495_cov_4.077869, whole genome shotgun sequence.
ACCESSION   DAOPBK010000263 DAOPBK010000000
VERSION     DAOPBK010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32959_length_2495_cov_4.077869"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1606
                     /gene="glyA"
                     /locus_tag="PLA17_10985"
     CDS             326..1606
                     /gene="glyA"
                     /locus_tag="PLA17_10985"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPK85977.1"
                     /translation="MERDSVIFELIDRERRRQTEGLELIASENFVSPQVLEAMGSVLT
                     NKYAEGYPRARYYGGCEVVDEVEQLAIDRLKKLFNAEYANVQPHSGAQANMAVFLTIL
                     KPGDTFLGLNLAHGGHLSHGSPVNSSGILYNPVAYGVKEETGRVDYDMMEEIALREKP
                     KLIVGGASAYSREWDYARMRAIADKVGAMLMIDMAHPAGLIAAGLLDNPLRHAHVVTS
                     TTHKTLRGPRGGIILMGTDFPNPWGVTTPKGEIKMMSAMFNSAVFPGIQGGPLEHVIA
                     AKAVSFGEALKPEFRTYQQQVKKNAAVMAEAFIALGYKVISGGTDNHSMLIDLRTRFP
                     EISGRKVENTLTRAHITLNKNMVPFDSRSPFLTSGIRVGTPAITTRGLKEGDMGTIVQ
                     LIDEVLVNIENESVIASVGAKVVKMMKDLPLFAY"
     gene            1640..2038
                     /locus_tag="PLA17_10990"
     CDS             1640..2038
                     /locus_tag="PLA17_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK85978.1"
                     /translation="MEKAGARTDILIYVVDDDPVVTSLVSGRLEGNGYSVRAFSYAED
                     CIAALGDIPDLIILDYYFLKEGETPMNGMEAYDEITRIAPEVPVVMLSAQEKGEVVLE
                     FARKGIADYVIKDTDLIDNLLMAVKDVLER"
     gene            2051..>2495
                     /locus_tag="PLA17_10995"
     CDS             2051..>2495
                     /locus_tag="PLA17_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPK85979.1"
                     /translation="MEWYMYLAVIAAGIFAGFVNTLAGGGSLLTLPLLMFLGLPANIA
                     NGTNRIGVFVQSLVSSISFKKQKLYTVGESLWMSVPAIAGALLGSLIAVKINERMMEL
                     LIGGLLVFMFFVILYKPEQWVRDHAHSPAMKRRWWVPVVFFIIGIY"
BASE COUNT          636 a          608 c          656 g          595 t
ORIGIN      
        1 accttcatat attgatatca gtgagagagg tgatgcagtc cttgacgcct ttgaacgcac
       61 cggagcctgg aacctccctg tgcttgacca gggccggtac gtgggattta tctccaagtc
      121 aagaatcttt tccgcatacc gcgaactgct caggcagttc tcagcagaat aatccggttt
      181 gtcaatatcc tgtctccggc gtgcttcaat gccatcaggg ggtcatgaaa aaatttgatt
      241 atataatgta tatttgcacg cagatttccg gggttcaccg gctcgagaaa actggtattt
      301 atcaaccgaa caaagattct taatcatgga aagagacagc gtaatatttg aactgatcga
      361 ccgcgaacgc agacgtcaga ctgaaggtct tgaactcatc gcatcggaaa acttcgtcag
      421 cccgcaggtg cttgaggcaa tggggtccgt gcttaccaac aagtacgcgg aaggttaccc
      481 cagggcaagg tattacggcg ggtgtgaggt tgttgatgag gttgaacagc ttgcaattga
      541 caggctgaag aaactcttca atgcagaata tgcaaacgta cagcctcact caggggctca
      601 ggcaaacatg gctgtcttcc tgacaatact caaaccgggt gataccttcc tgggattgaa
      661 tctggcacat ggaggtcacc tctcgcacgg gtctccggtt aactcatcag gcatactgta
      721 taatccggtc gcttacgggg tgaaggagga gaccggccgt gttgactatg atatgatgga
      781 ggagattgcc ctccgcgaaa aaccaaaact gatagtgggc ggtgcttctg catactccag
      841 ggaatgggac tatgcaagga tgcgcgctat tgccgacaag gtcggggcta tgctcatgat
      901 tgacatggcg caccctgccg gccttatagc agcaggattg ctcgataacc cgcttcgcca
      961 tgcacatgtt gttacctcaa caacacataa aaccctcagg gggccacgcg gaggaattat
     1021 cctcatggga accgacttcc ccaacccatg gggcgtgacc actcccaagg gcgagatcaa
     1081 aatgatgtcg gcaatgttta actcagccgt gttccccgga atacagggcg gaccactgga
     1141 gcatgttatc gcagccaaag ccgtctcatt cggagaagcg cttaaacctg agttccggac
     1201 ctaccagcag caggtgaaga aaaatgccgc agtgatggcc gaagcattca tcgccctggg
     1261 ttacaaggtg atctcgggcg gaacagacaa ccattcgatg ctgatcgacc tgagaacacg
     1321 cttcccggaa atttcaggca ggaaggttga aaataccctg acaagggcac acatcacgct
     1381 caataaaaac atggtgccgt tcgactcccg ctcgccgttc cttacctcag gaataagggt
     1441 aggtacccct gccatcacaa cccgcggact gaaggagggt gacatgggaa ccatcgttca
     1501 gctcattgat gaggtactgg ttaacattga aaatgaatca gtgatagcat cagtgggagc
     1561 aaaggtggtg aaaatgatga aagacctccc tctttttgca tactagtaaa tttgtaattc
     1621 cttttgtagt tttgcagtta tggaaaaagc tggcgcccgg actgacatcc ttatatatgt
     1681 ggttgatgat gatcctgtcg ttaccagcct ggtctcaggc aggctggaag gcaatggtta
     1741 ttctgtgagg gcattttcct atgccgagga ttgcatcgct gcactcggtg atattcctga
     1801 cctgatcatc cttgactact atttcctgaa agagggtgaa actcccatga atggaatgga
     1861 agcctatgac gaaattacca ggattgctcc ggaagttccc gtcgtcatgc tttcagccca
     1921 ggagaaggga gaggtggtcc tcgagtttgc acgaaagggg atagctgact acgtcataaa
     1981 ggatactgac ctgattgaca atcttctgat ggcagttaag gacgtcctgg aaagataggc
     2041 tccggcaaat atggaatggt acatgtacct ggctgtaata gccgccggca tctttgcagg
     2101 ctttgtcaac actcttgccg gcggcggctc actgcttacc ctgcctctcc tgatgttcct
     2161 cggcctgcct gccaatattg caaatggcac caaccgaatc ggcgtcttcg tccagagcct
     2221 ggtcagcagc ataagcttta aaaagcagaa actctatacc gtcggcgaaa gcctatggat
     2281 gtcagtgcct gccattgcag gagccctgct ggggtcactc attgcagtga agatcaatga
     2341 acggatgatg gaactgctga taggaggact gcttgtcttc atgttcttcg taatccttta
     2401 caagcctgaa cagtgggtac gtgaccatgc tcactcaccg gccatgaagc ggcggtggtg
     2461 ggtacctgtg gtgtttttca tcatcggcat atatg
//
LOCUS       DAOPBK010000264         2345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_35798_length_2345_cov_4.445415, whole genome shotgun sequence.
ACCESSION   DAOPBK010000264 DAOPBK010000000
VERSION     DAOPBK010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35798_length_2345_cov_4.445415"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="PLA17_11000"
     CDS             <1..1230
                     /locus_tag="PLA17_11000"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPK85980.1"
                     /translation="QAMIFALQRCDKAREAVLLIGDLMIRYGFLPSSGDGSEALVIAD
                     TEEAWVFEVFGVGNGWEPGSGKPGAIWAAQRLPDDQATMVPNWSIIKEIDAGDKSQFL
                     VSDNYMKEAIDRGWYDPASGKPFVWQEAYAPLPEEFATGRFWLFFSTFMPNLKQWPDR
                     MLDPTNPYKGMEPYFQVVEPLSIYPFAAVPEKKISVRDVIAFQRSTFEGTIYDMTSYP
                     EWLVPDGKGGYVKSPLATPFPGSEMRKLIKQTYRRPVARHRGHYGMVMQLRDWLPDAI
                     GGVYWVYLDNPYFSPYVPIYAGNLSVAETYNIYDPEKYDERSARWAIDFVDNLANLRF
                     RDIAADVRAVRDPFEEEIFAGQEKTEKEALEMYKKNPETARKFLTAYSDAKMNAVTKM
                     FIELRDQIITRYTNNRE"
     gene            complement(1332..1406)
                     /locus_tag="PLA17_11005"
     tRNA            complement(1332..1406)
                     /locus_tag="PLA17_11005"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1369..1371),aa:Pro,seq:ggg)
     gene            1562..>2345
                     /locus_tag="PLA17_11010"
     CDS             1562..>2345
                     /locus_tag="PLA17_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85981.1"
                     /translation="MNQVNRRNFLRLAGAGAAAAAAAPVAQAMGAATATAASVVNPEN
                     ADRETQQDKPDTNIADAAKIARGPNSMPGLYPGRVVMVKNSRSVRDNLIVEQEVYDMI
                     ARSMLELTGEKKLKKAWRKFVKPGERIGLKVNPVAGKSLSTSHEVVRAVIAQLEESGI
                     KRSQLTIWDRREFELTDVGFTQENYPGIRITGTEQMDKEGLYYGKDGKLYGEHMIDRD
                     WYYWADVEGEYDEYTMPYMVNGGKYSYFTKIVTQELDRIINIP"
BASE COUNT          620 a          559 c          655 g          511 t
ORIGIN      
        1 caggctatga tctttgccct gcagcgttgt gacaaggcaa gggaagcagt gttgcttata
       61 ggggacctga tgatccgtta cggtttcctg ccgtcaagcg gagacggttc tgaggcgctg
      121 gttattgctg acacggaaga ggcatgggtt ttcgaagtat tcggggtagg caacggctgg
      181 gagccgggga gcggcaaacc cggtgccatc tgggcagctc agcggcttcc cgatgatcag
      241 gcgacaatgg tgccaaactg gagcattatc aaggagattg atgccggaga caaaagtcag
      301 ttcctggtct ctgacaacta tatgaaagag gcaattgacc gtggctggta tgacccggcc
      361 tccgggaaac ctttcgtgtg gcaggaagca tacgcaccac tgcctgagga gtttgccacc
      421 ggccgcttct ggcttttctt cagcaccttt atgcccaacc tgaagcagtg gcctgacagg
      481 atgcttgatc ctacaaatcc gtacaagggg atggaaccct acttccaggt ggtggagcct
      541 ctttccatct acccgtttgc agctgttccc gagaagaaga tttcagtcag ggatgtaatt
      601 gctttccagc ggtcaacctt cgagggtacc atttatgaca tgacttcata cccggagtgg
      661 ctggtgcctg acggtaaggg cggatatgtc aaaagcccgc ttgccacacc tttcccgggg
      721 agtgagatgc ggaagctgat taaacagacc tacagaagac cggtagcccg tcacagaggt
      781 cactacggca tggtgatgca gcttcgcgac tggcttccgg atgccattgg cggcgtgtac
      841 tgggtctatc tcgacaaccc ttacttcagt ccgtatgtac ctatttatgc cggaaacctt
      901 tccgtggcag agacttataa tatctatgat ccggagaaat atgacgaacg ctctgcgcga
      961 tgggcgatag atttcgttga taaccttgca aaccttcggt tcagggatat tgctgcagat
     1021 gtccgtgcgg tgagggatcc gttcgaggag gagatttttg ccggtcagga gaagacggag
     1081 aaggaggctc ttgagatgta taaaaagaat ccggaaactg caaggaagtt ccttaccgca
     1141 tactctgacg cgaagatgaa cgccgtaacg aagatgttca ttgaactccg tgatcagata
     1201 attacccggt acaccaataa cagggagtag aaggcattgg agagactttc ccggaaacag
     1261 gcgctttccg gtcaccctta aaagcaaaaa atccacccgc caattacggc gggtggattt
     1321 tttctgctca gtcgggatgc cgggatttga acccggggcc ccttgccccc cagacaagta
     1381 ctctaaccgg gctgagctac atcccgtgct tttaaagcgg tgcaaaatta attaacaatt
     1441 ctgattatgc aaaaaaagct gaggcctgct atccggcata aaaaatgaaa tatgactcac
     1501 cggtacaaac atttatattt gtttgtcctt taaatacgtt atcatctcac aaagaagaat
     1561 catgaatcag gtcaatcgcc gtaacttcct gcgcctggcg ggtgcaggag ccgcagccgc
     1621 tgcagcagca ccggtagctc aggcaatggg agcagcaacg gccaccgctg ccagtgttgt
     1681 gaacccggag aatgctgacc gggagactca gcaggacaaa cctgacacca acatagctga
     1741 tgctgcaaag attgccaggg gtccgaattc gatgcccggt ctctatcccg gacgcgtggt
     1801 tatggtgaaa aacagccgtt cagtcaggga taacctcata gtggagcagg aggtatatga
     1861 catgatagcc cgctcaatgc tcgaactgac aggagagaaa aaactgaaaa aggcctggag
     1921 gaaatttgtc aaaccgggcg aaagaatcgg cctcaaggta aacccggtgg caggaaagag
     1981 cctcagcacc agccatgagg tggtgagggc ggtgatagcc cagcttgagg agagcggcat
     2041 aaagagatcg cagcttacaa tatgggatcg tcgtgagttt gaattgacag acgtgggatt
     2101 cacacaggaa aactacccgg gaatacgcat cacaggtacc gaacagatgg ataaggaggg
     2161 tctctactac ggcaaggacg gcaagctcta cggtgagcat atgattgacc gcgactggta
     2221 ctactgggct gacgttgagg gtgagtatga cgagtacacc atgccttaca tggtcaacgg
     2281 aggcaagtat tcatacttta ccaaaattgt aacgcaggag cttgacagga tcatcaatat
     2341 cccca
//
LOCUS       DAOPBK010000265         2343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_35844_length_2343_cov_3.456294, whole genome shotgun sequence.
ACCESSION   DAOPBK010000265 DAOPBK010000000
VERSION     DAOPBK010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35844_length_2343_cov_3.456294"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="PLA17_11015"
     CDS             <1..1435
                     /locus_tag="PLA17_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85982.1"
                     /translation="ATSNPAALSETSFGNISMAQAGYTGSDNDIRLTQQPVQSSSYHA
                     VTKGYMKLGRVSMFGSFGYSNSGYKGVNYNGTMMFDTFNPYLVGDSVPARQLREQFVM
                     GGKFSYLLNDRISLAVGAGYLSAVGARQKDPRNKNTISALKVTPGITYDMGKTKVGLS
                     GSVYTSSNEMSYRVEGNWNQNLFVFLGLGYFRQEINISSYSQWYVGHGYAGALQVSHE
                     NGDVYIIAELGYDHYTEEARSGSSFRLIDGITETDGISLSGMLRIERGKAFHVLTVKG
                     SLKALSGDEILQRSYRINRGTYSYDSLATVSWIKNKQMITDLGGEVKYTRLVYDADYN
                     IDVEFGGALQAGYYSTEHFPVQSYGFYNTFNLGGSLFARKLLRAGRLLITPGLEAGYR
                     MNLGSDMSYTVQPLSFPAMVYHDYYISKANIISASASVRIEMPFSQNKFVKSLFLIPG
                     GRYAAAPGSEAGDLSGYLIDVVAGLTF"
     gene            1440..>2343
                     /locus_tag="PLA17_11020"
     CDS             1440..>2343
                     /locus_tag="PLA17_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85983.1"
                     /translation="MKKSIVSAMLALILLTPSFSQPLKGEFAIIADSKSLAVAADQIR
                     DYAAVLRTEGLDAVVIEDRWKHADSIRSLLRDMYVSGHGLEGAVFIGDIPVPMLRDAQ
                     HLTSAFKMDQERYAWDRSSVPSDRFYEDFDLEFTFLKADTNDLYFYYSLNPSSPQMLT
                     PDIYSGRIRIPDDSDGSKLRAYLLKVIEAHRNPDIVDELLFFAGHGYNSNSLTARIDE
                     KAALLQQFPQFNRQDRGLEYIDYTFDEHIKNSILSLMTDEMVDIALLHHHGAYDAQYV
                     GSEKAANGVQQNIESIKYYLRSKIR"
BASE COUNT          610 a          569 c          598 g          566 t
ORIGIN      
        1 agccacttca aacccggccg cactgtccga aaccagcttc ggtaacatca gcatggcaca
       61 ggccggatat accggctcag ataatgatat aaggctgacg cagcagcccg tgcaatcctc
      121 ttcataccac gctgttacaa aggggtatat gaaactcgga agagtttcca tgttcggatc
      181 attcggatat tccaactccg gttacaaggg tgtcaattat aatggcacca tgatgttcga
      241 tacattcaat ccatacctgg taggagattc ggtccctgcc aggcagttga gggaacagtt
      301 cgtcatggga ggcaagttca gctatctgtt gaatgaccgt atcagcctgg cagttggggc
      361 cgggtacctc tctgctgtag gagccaggca gaaggacccg cgcaacaaga acaccatctc
      421 cgcgctgaaa gtcactcctg gcataaccta cgacatgggt aaaacaaaag tcggcctctc
      481 tggttcggta tatacatcca gcaatgagat gtcttacagg gttgaaggta actggaacca
      541 gaacctgttt gtctttttgg gcctcggtta cttcagacag gaaatcaaca tctcatccta
      601 ttcacagtgg tatgtgggcc atggttatgc cggagccctg caggtttccc atgaaaacgg
      661 tgacgtttat ataatagcag agttaggcta tgatcattac acggaagaag cccgctcagg
      721 aagctccttc agactgattg acggcatcac cgagacagat ggcatctcac tgtcagggat
      781 gctcaggatt gaacgcggaa aggcttttca tgtcctcacc gtcaaaggat cattgaaggc
      841 attgagcggc gatgagatac tgcagcgatc ttacagaata aacaggggca cctattccta
      901 tgattcgctt gcaacggtaa gctggataaa gaataaacag atgatcactg acctgggtgg
      961 tgaggtgaag tatacccgac tggtttatga tgccgattac aatattgacg tggaatttgg
     1021 gggagcactg caggccggct attactcaac cgaacacttc ccggtgcaga gctacggatt
     1081 ttacaataca tttaacctgg gcggatcact ctttgcaagg aaactcctga gagcgggacg
     1141 gctgctcata actccgggcc tggaggcagg ctacaggatg aaccttgggt ccgatatgtc
     1201 ctataccgtt cagccattga gcttcccggc tatggtatac catgattatt atatctccaa
     1261 ggcaaatatt atcagtgcat cagcatccgt caggattgag atgcctttca gtcagaacaa
     1321 atttgtaaaa tcattgttcc tgattccggg ggggcggtat gcagcagctc cgggctctga
     1381 ggccggtgat ctgtcaggat acctcataga tgttgtggcc ggacttacat tctgaaatca
     1441 tgaagaaatc gatcgtatca gcaatgttgg cgttaatcct gttaacgcct tcattttctc
     1501 agcccctcaa gggcgaattt gcaatcatag ccgatagcaa atccctggct gtggcagctg
     1561 accagatcag ggattatgca gccgtgctcc gtacagaagg actggatgcg gttgtaatag
     1621 aagaccgctg gaaacatgct gactcaatca ggtcactgtt gcgtgatatg tatgtctcag
     1681 gccatggcct ggagggagcg gtcttcatcg gcgatattcc tgtacccatg ctccgtgatg
     1741 cacagcacct cacctccgcc ttcaagatgg atcaggagag atatgcgtgg gacagatcct
     1801 cggtaccttc tgacaggttc tatgaagact ttgatctgga gtttacattc ctgaaggctg
     1861 acacaaacga cctctatttt tattacagtc tgaatccctc atcaccgcag atgctgacac
     1921 cagacatcta cagcggccgc atccgcattc ctgacgacag tgacgggtca aagctccggg
     1981 cttacctgct gaaagtaatt gaagcacaca ggaacccgga tatcgttgat gagttgctct
     2041 tcttcgccgg gcatggatat aattccaact ccctgacagc ccgtattgat gagaaggctg
     2101 ccctgctgca gcagtttccg cagttcaaca ggcaggacag agggctggaa tacattgatt
     2161 acacctttga tgagcacatc aagaacagca tactctctct gatgactgac gagatggttg
     2221 acatagcgct gctgcatcat cacggtgcct acgatgccca gtatgtggga tcggagaagg
     2281 cggccaacgg tgtgcagcag aatatcgaaa gcattaagta ctacctgcgt tcaaagataa
     2341 ggg
//
LOCUS       DAOPBK010000266         2251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_37828_length_2251_cov_4.174408, whole genome shotgun sequence.
ACCESSION   DAOPBK010000266 DAOPBK010000000
VERSION     DAOPBK010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37828_length_2251_cov_4.174408"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLA17_11025"
     CDS             complement(<1..799)
                     /locus_tag="PLA17_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85984.1"
                     /translation="MRLIYKSAVLTAVVTTALLMTSCAVTGNVTDTKDMAYLYNPTQS
                     IFTPMISVYNEDAETSVLNISIRKGELYFSEANPEGVPMASLLVSVRLFDNTFGGVLA
                     DTSTFKYDIKREEVGGEYSFRTPLSAHDGNSYTAEIKIIDLIRQRTRQLFIDFERTGR
                     YSGLSYKVRDHFTKNEVFSRTVKVDQYVNILAPSLQPDTLWLFYYKAVTAIPPSPSTI
                     LPEVTIAPEPEAIVPLAWSDTLPLMFPNPGIYLISVDSLIREGLVLCN"
     gene            complement(827..1585)
                     /locus_tag="PLA17_11030"
     CDS             complement(827..1585)
                     /locus_tag="PLA17_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPK85985.1"
                     /translation="MILFERKYPVNIFNTDLNGRLSPGALFSFFEDLAGRHAAEIGWG
                     RDDLMASGGYFWALSRILMKIERLPKAWDEVTLRTWPRGTETIFALRDLEMYDEAGNR
                     IAGASTSWVIVDYNTRKAQRPDKALSNLNLQFPEARALDTNARKVPSLPPGDHRLTRL
                     KVKIDDIDVNRHVNNARYVYWAVNCYEPEFITLHTPDTIEANYLLEGHHDEMINILTA
                     PCEGEENAYIHSVTRESDGAELCRLRMSWKENRQ"
     gene            complement(1572..>2251)
                     /locus_tag="PLA17_11035"
     CDS             complement(1572..>2251)
                     /locus_tag="PLA17_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HPK85986.1"
                     /translation="NRKIRTHLGGIAEFLRARKIESLRELERKSPATGKGYTANAKGS
                     TAAGKGYAAKGNMPAGKGGAAAAKGKTAQAKGKPVTGKGNVAAGPVKAANAKGSMAAG
                     KGSTAPGENEVSAANDNQGMTKMLPQTKSGTSYQEKKENERVHRRLKKKITEAEAQIT
                     ATEHEIAEMDVRLASGDPAVISDPAFYSAYEEKKNRLEALMEKWEDAHNELESFQREY
                     MNNYDTV"
BASE COUNT          440 a          595 c          582 g          634 t
ORIGIN      
        1 agttgcagag cacgagaccc tcacggatca ggctgtcaac ggagatcaga taaatgcccg
       61 ggttagggaa catgagggga agggtgtctg accatgccag tggaacgatt gcttcaggtt
      121 ccggcgctat tgtcacctcg ggaaggatgg ttgacgggga cggggggatt gccgtaacag
      181 ccttgtaata gaacagccac agcgtgtcag gctgcaacga cggggcgaga atgttgacat
      241 actggtcaac cttcactgtg cggctgaaca cttcgttttt ggtaaaatgg tcacgcacct
      301 tatagctgag accgctgtac ctgcctgtac gctcgaaatc aatgaacaac tgccgggtac
      361 gctgcctgat gaggtcaatg atcttaattt cagcagtgta tgagttgccg tcatgtgcag
      421 acaagggggt ccggaatgag tattcaccgc caacctcctc acgtttgatg tcatacttga
      481 aggtcgatgt atctgccagc actcctccaa aagtattgtc aaagagcctt accgagacga
      541 gcaatgaagc cattggcaca ccttcggggt ttgcctcgct gaagtagagt tcacccttgc
      601 ggatgctgat gttcagaaca gatgtctctg catcctcatt gtacacggat atcatggggg
      661 tgaaaatgct ctgcgtcggg ttgtataaat atgccatgtc ttttgtgtca gtgacgtttc
      721 cggtcactgc acatgaagtc atcagcaggg ccgttgtcac aaccgctgtg agtactgctg
      781 acttatagat gaggcgcatt gtcatggcat aaaggtaaaa aagatttcat tgcctgttct
      841 ctttccagct cattctcaga cgacagagct cggcaccgtc actctcgcgg gtaacggagt
      901 gtatgtatgc attctcctcg ccttcgcagg gggcggtcag gatatttatc atctcatcat
      961 gatgcccttc gagaaggtag ttcgcctcga tggtgtcagg agtatggagg gtaatgaatt
     1021 ccggttcata gcagttcaca gcccagtaca catacctggc gttgttcaca tgtctgttga
     1081 cgtcgatgtc gtcaatcttg accttcagcc ttgtcaggcg atgatcacct ggtggcagcg
     1141 atggcacttt tcgtgcgttg gtatcaaggg ctctggcttc ggggaattgg aggttcaggt
     1201 tcgagagcgc cttgtcagga cgctgagcct tgcgtgtgtt gtaatcgaca atcacccatg
     1261 acgttgaggc gccggcgatt ctgttgcctg cttcatcata catttcaagg tcacgcaggg
     1321 cgaaaatagt ttcggtgcct cgtggccatg tgcggagggt gacctcgtcc catgctttcg
     1381 ggagacgttc tatcttcatc aggatacgcg aaagcgccca gaagtaacca cccgatgcca
     1441 tcaggtcatc acggccccag ccaatctctg ctgcatgacg gcccgccagg tcctcgaaga
     1501 agctgaagag tgcaccggga ctaagccgtc cgttgaggtc ggtattgaag atgttgaccg
     1561 ggtatttcct ttcaaacagt atcatagttg ttcatatatt ccctttggaa gctctcaagc
     1621 tcgttatgtg catcctccca cttctccatc agtgcctcaa gcctgttctt cttctcctcg
     1681 taagcgctgt agaaggcagg gtcacttatc actgccgggt ctcccgaggc gagacgtaca
     1741 tccatttctg ctatttcgtg ttccgtggct gttatctgag cttcggcctc ggtgatcttt
     1801 tttttaagcc ggcgatgcac acgttcgttt tccttctttt cctgatacga ggtgccagat
     1861 ttagtctggg gcaacatctt cgtcataccc tggttgtcat ttgctgctga cacttcgttt
     1921 tccccaggtg cggtgcttcc ctttcctgct gccatgctac ctttggcatt cgctgccttt
     1981 actggtcctg ctgccacgtt gcctttccct gtcaccggtt tacccttcgc ctgtgctgtc
     2041 tttccttttg ctgctgccgc gccacctttc ccggctggca tgtttccttt cgctgcgtaa
     2101 ccctttcctg ctgccgtgct acctttggca ttcgctgtgt aaccttttcc tgttgccgga
     2161 ctcttccttt ccagttctct gagcgactct atctttctgg ccctgaggaa ctcagcgatc
     2221 cctccgagat gcgttctgat cttcctgtta c
//
LOCUS       DAOPBK010000267         2233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_38232_length_2233_cov_2.880624, whole genome shotgun sequence.
ACCESSION   DAOPBK010000267 DAOPBK010000000
VERSION     DAOPBK010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38232_length_2233_cov_2.880624"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLA17_11040"
     CDS             complement(<1..1104)
                     /locus_tag="PLA17_11040"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPK85987.1"
                     /translation="MYSPEFRLLLEACRFRGCGTDISSAYEIAERESPDWNVLVESTS
                     FHRIGPQMLSLLGMIPGGVVPSPVLDKLKDTVQDNLVRQLRYVSEFFHISEWLAEEGI
                     EAIPFKGFWLGESAYGDIGARESSDIDIFIDLKNLEAVKRIMTSKGYEGHETLDLLTD
                     DYVRREIAEYNFGRYEEGVRVAHIEFHWRSAMTFYRMDISGNDLRSQVMPGILQGREI
                     PVFSPAANLLLAVMHHGGKDCYWQLRQVLDIAHILRRNPDLDAEWLLAQAERFHVTTL
                     LLLGVRLAHELTGVEVPEAFAGCLSDKRLERIVRGRFMLMALPLAGLELYKDRLASWL
                     FKIRSRDGLWVKGSMAIYTLRKVIAPRMVPERWR"
     gene            complement(1154..>2233)
                     /locus_tag="PLA17_11045"
     CDS             complement(1154..>2233)
                     /locus_tag="PLA17_11045"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPK85988.1"
                     /translation="AFASALIITWYYIPRVIKVVKERHLEDKPGFHKIHNSEVPTLGG
                     IGIFGGFIFGFLIGINGYIPGLSYFTAAVVLLLFVGIKDDLVYLNPKKKFWGELGAAV
                     LVALFTNIHITHFHGFMGITDISSTASFIVTVLLFLFIINAVNLTDGIDGLAASVGII
                     ASVAFGVFFFLSGDYGYTVMAAALLGSLLAFLYYNLSQGPKKIFMGDSGSLVIGFTLA
                     VLAIRFNELVASGESVLKLKSAPAVSIAVLILPLFDMIRVTVLRMLDHKSIFIGDKRH
                     IHHMMLRAGLSHRQATLYMSLFNIFIIGVAFLFDSLGIFLLGLLLLTLCLVATGILSV
                     IVRRKSTPGPKMVKVSEMAEVTGLN"
BASE COUNT          645 a          616 c          480 g          492 t
ORIGIN      
        1 tcgccaccgt tcaggtacca tccgaggcgc aataacctta cgcaaggtat aaatggccat
       61 cgagcctttt acccacagtc cgtcacgtga acgaatctta aacaaccacg aggcaagcct
      121 gtctttatat aattccagcc ccgccagcgg cagagccatc aacatgaacc ttccccggac
      181 tattctctcc agcctcttgt ctgaaagaca accagcaaac gcttcaggta cctcaacccc
      241 cgtcagctca tgagcaagcc tgactcccag caacaggagg gtggtaacat gaaacctttc
      301 tgcctgagct aaaagccact cagcatcaag atcaggattt ctcctgagta tgtgagctat
      361 atcaagcacc tgtctcagtt gccagtaaca atccttcccg ccatgatgca taacagccag
      421 cagaagattg gcagcaggag aaaataccgg aatctcacga ccctgcagta tccccggcat
      481 aacctgcgac ctgaggtcat taccggaaat gtccatcctg taaaaggtca tcgcactgcg
      541 ccagtgaaac tcgatatgag ccacacggac cccctcttcg tacctcccga agttgtattc
      601 tgcaatctct ctcctgacat aatcatcggt gagcaggtcc aacgtctcat gaccttcata
      661 gccttttgaa gtcattatcc tcttcaccgc ttcaagattc ttcaggtcaa tgaagatgtc
      721 aatgtctgat gactcccttg cgccaatgtc accgtaagcc gactccccca gccagaatcc
      781 tttaaaaggt attgcttcaa tcccttcttc tgccagccat tcgctaatat gaaaaaactc
      841 cgagacatac ctcagttgcc ttacaagatt atcctgaacg gtatccttca gcttgtccag
      901 caccggcgaa ggcaccactc ctccgggaat catccccagg agggagagca tctgtggccc
      961 tatcctgtgg aaggatgtgc tctcaaccaa aacattccag tcggggctct ctctttctgc
     1021 aatctcatat gcagatgaaa tatcagtccc gcaaccacga aaccggcatg cctccagcag
     1081 aagtctgaat tcaggggaat acatcagcga acaaataccc tggcgaatgc agggaacaaa
     1141 aaaacggtgc ctgtcagttc aggccggtta cctcagccat ctctgaaacc tttaccatct
     1201 ttggcccggg agtgcttttt cttctgacaa tgacactgag aatccctgta gcaacaagac
     1261 acagggtaag cagcagaagc ccaagcagaa atatccccag gctgtcaaaa aggaaggcaa
     1321 cgcctataat gaatatattg aacagcgaca tatataatgt agcctgccgg tgactcaggc
     1381 cggcacggag catcatgtga tggatatgcc gcttgtcacc tatgaagata cttttatggt
     1441 caagcatcct gagaaccgta acccttatca tgtcaaagag aggaagtatc agcactgcta
     1501 ttgagactgc cggcgcggac ttcagcttca gaaccgactc gcctgatgcg acaagttcgt
     1561 taaaacgtat tgcaagaacc gcaagggtaa acccgatcac cagcgaaccg gaatcaccca
     1621 tgaaaatctt cttcggaccc tgtgacaggt tgtagtatag gaaggcaagc aatgaaccca
     1681 acagggctgc cgccataacc gtgtacccgt agtctcccga gaggaagaag aagacaccga
     1741 atgccacgga ggcaatgata cccactgatg ccgcaagccc gtcaatcccg tcagtcagat
     1801 tgactgcatt aattatgaat aaaaacaaca ggacggtaac aatgaatgag gctgttgaag
     1861 agatatcggt tatgcccata aatccatgaa aatgagtaat atggatatta gtgaagaggg
     1921 caacaagaac ggctgccccc agctctcccc agaatttttt cttcggattg agatagacca
     1981 ggtcatcctt tatcccgacg aacagcagta atactaccgc agcggtaaaa tatgatagcc
     2041 cgggtatgta cccatttatc cctatcagga aaccaaagat aaaacctccg aaaatgccga
     2101 tgccacccag ggtgggcacc tcggaattat gaatcttgtg gaatccgggc ttgtcctcga
     2161 ggtgacgctc tttgactacc ttaattaccc tgggtatata ataccaggta ataataagcg
     2221 cactggcaaa ggc
//
LOCUS       DAOPBK010000268         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_39985_length_2160_cov_3.649406, whole genome shotgun sequence.
ACCESSION   DAOPBK010000268 DAOPBK010000000
VERSION     DAOPBK010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39985_length_2160_cov_3.649406"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2160)
                     /locus_tag="PLA17_11050"
     CDS             complement(<1..>2160)
                     /locus_tag="PLA17_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85989.1"
                     /translation="PDYVYAYIESKPSAIYRSTDGGVNWEKRGEKNIGNRPFYYAEIY
                     VDPKNENRIYTLFSGVNVSDDGALTFDRRTAESVHLDHHAWYIDPLNPDRMLDGNDGG
                     MGITYDRGVTWRHIENLPAGQFYHINVDNETPYNIYGGLQDNGSWRGPAYAWYSGPLI
                     NEMYDFLIGGDGFDAMPVPGDSRYCYAQSQGGALRRMDVTTGWNKSIRPQAEAGEQLR
                     FNWNSALAQDPFDNNTIYFGSQFVHKSSDRGDSWIKISPDLTTNDPEKQKQDQSGGIT
                     LEATGAENHCTILTISPSSLRQGVIWAGTDDGMIQLTEDGGRNWRNTSRNIKGMPRNG
                     WVAQVTASLHDPGEAFAVVNDYRQGDNAAYLFRTRDYGKSWQRIIDDTDVWGYVLCFV
                     QDPVEPKLMFAGTEYGLYVSFDQGETWNRWTSGYPTVSTYDMVIHPKEHDLVIATFGR
                     AVWILDDIRPLRALASSGRKLLSEPLTLFDAPDAYMVSTKNLPGYYFYGDAMYRGENR
                     EQGAMITFYNGSDTGRVSVRLTDEAGNVVRAMQVNAKKGFNRFMWRFDRDPLPQLEYP
                     SGQTQQQDSRIRYYRGLGGSVIPGNYTVTLSSGSASSTAKVKVNHDPRMDAPDTDALK
                     KNYERARAIGSRIESINERLKPLAQVRESLAKSEGLIKENLGFAAAVTKEYKAVKEEL
                     ARMDEAFTNRQNGLSVRIGGYRVLLSASAPMTAQENEA"
BASE COUNT          455 a          601 c          535 g          569 t
ORIGIN      
        1 gcctcattct cctgtgcagt cataggagct gaggcactga gcagcaccct gtaaccgcct
       61 atcctgactg agagcccatt ctgcctgttg gtaaatgcct cgtccatcct tgcaagttcc
      121 tctttgacag ctttatactc tttagtcaca gcagctgcga agccgaggtt ttctttgatc
      181 agaccctcac tcttcgcaag gctctcgcgc acctgtgcca atggtttgag ccgttcattg
      241 attgattcga tccgtgaacc gatagccctg gctctctcat agtttttttt cagtgcatca
      301 gtgtcaggag catccattct cgggtcatga ttcaccttca ccttcgccgt cgaactggcg
      361 ctgccggatg agagagtgac agtatagtta ccggggatta cactcccgcc aagacccctg
      421 tagtatctta tcctggaatc ctgctgttga gtctggcctg aagggtattc aagctgcggg
      481 agcggatcac ggtcaaaacg ccacatgaac ctgttgaatc ccttttttgc attgacctgc
      541 attgccctta ccacgtttcc ggcttcatca gtcagcctga cagaaaccct gccggtatct
      601 gaaccgttgt agaatgtgat cattgctccc tgctccctgt tctcaccgcg gtacattgcg
      661 tcaccataga aatagtaacc ggggagattt tttgtcgata ccatgtatgc atcaggcgca
      721 tcaaagagag tcagcggctc tgagagcagc ttcctgcctg acgaagccag tgcccgcagg
      781 ggcctgatat catccagtat ccacacggca cggccgaagg ttgcgatcac caggtcatgc
      841 tccttcggat gaatcaccat atcatatgtt gaaaccgtgg ggtatccgct tgtccatctg
      901 ttccatgttt caccctggtc aaaggagaca taaaggccgt actcggtacc ggcaaacatc
      961 agttttggtt caacgggatc ctgcacgaag catagcacat aaccccatac atcggtatca
     1021 tcaatgatcc gctgccagct ctttccatag tcacgtgtcc ggaagaggta tgctgcattg
     1081 tctccctggc ggtagtcatt caccaccgca aaggcttcgc cgggatcatg cagcgaggca
     1141 gttacctgcg ccacccagcc gttgcgcggc atccccttga tgttacggga ggtgtttctc
     1201 cagttgcggc ctccatcttc agtcaactgg atcatgccgt catcggtgcc tgcccagata
     1261 actccctgcc tgaggctgct gggggagatt gtcagtatgg tacagtggtt ttcagctccg
     1321 gtggcttcaa gggtaatacc cccgctctga tcctgtttct gcttctccgg gtcattggtg
     1381 gtaaggtccg gagaaatctt tatccaggag tcgccccggt cgctgctctt atgcacaaac
     1441 tggctcccga aatagattgt gttgttatcg aagggatcct gtgccagtgc ggagttccag
     1501 ttgaaacgaa gctgttcgcc tgcttcggcc tgtggcctga tactcttgtt ccatccggtg
     1561 gtgacatcca tccttctcag agctccgccc tgtgactggg cataacagta ccggctgtca
     1621 ccgggcaccg gcatggcgtc aaaaccgtcg ccacctatca ggaaatcata catttcattg
     1681 atgagggggc cgctatacca ggcataggcc gggcctctcc atgagccatt gtcctgaaga
     1741 ccgccgtaaa tattgtaagg tgtttcatta tcaacattaa tgtgatagaa ttgtcctgcg
     1801 gggaggttct caatatggcg ccatgtgaca cccctgtcat aggtgatgcc catccctccg
     1861 tcattgccgt caagcatgcg gtcaggattc aggggatcaa tgtaccaggc atggtgatca
     1921 agatggactg attctgcggt acgccggtca aaggtgagcg ccccgtcatc actcacattg
     1981 acacctgaga agagggtata gatcctgttc tcattcttcg ggtcaacata tatctcggca
     2041 tagtaaaacg gcctgtttcc tatgttcttt tcaccgcgct tttcccagtt caccccgccg
     2101 tcggtggaac ggtatatggc tgacggtttt gactctatat aggcatatac ataatcagga
//
LOCUS       DAOPBK010000269         2115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_41165_length_2115_cov_3.008738, whole genome shotgun sequence.
ACCESSION   DAOPBK010000269 DAOPBK010000000
VERSION     DAOPBK010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41165_length_2115_cov_3.008738"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1149
                     /locus_tag="PLA17_11055"
     CDS             148..1149
                     /locus_tag="PLA17_11055"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPK85990.1"
                     /translation="MAYNLHNRSFLKLLDFTPEEIQFLLDLSFDLKKAKYAGTEQMRL
                     KGKKIALIFEKTSTRTRCAFESAAFDQGAHVTYLGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQKIVEELAAHAGVPVWNGLTDEFHPTQVLADFMTMLEHSDKPLNKISFC
                     YLGDARNNMGNSLMAGAAKMGMDFRSAAPRACQPADDLVKKCREIAAETGAKITLTED
                     VAEAVRGVDFLYTDVWVSMGEPDSVWEERIGLLKPYQVNSEVIRLTGNPNVKFLHCLP
                     AFHNRETKVGEEIYKKYGLDGMEVTEEVFESSHSIVFDEAENRMHTIKAVMVATLGE"
     gene            complement(1262..1738)
                     /locus_tag="PLA17_11060"
     CDS             complement(1262..1738)
                     /locus_tag="PLA17_11060"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPK85991.1"
                     /translation="MDYLLLALAIILMILGIAGCLLPVLPGPPLTYLGLVVLHFTSFA
                     EISKNLFIILGVVAIVVTVIDYVVPIWGTRQFGGSKYGMRGATVGLIIGLFLGPVGII
                     IGPFIGAVVGELIFKDDIRYALKAGFGSLLGFLTGVGLKLAAALLMTFYFIRALVI"
     gene            complement(1775..>2115)
                     /locus_tag="PLA17_11065"
     CDS             complement(1775..>2115)
                     /locus_tag="PLA17_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPK85992.1"
                     /translation="EEVFTKVVRAGKRTYFFDVKSTRHEDLYLTITESKKRPGKEGKM
                     FYEKHKIFLYKEDFEKFSEGLHEAITFADNGTAVSTHASPETEDCGETVTGVAATEFS
                     DVDFDELGTR"
BASE COUNT          589 a          503 c          547 g          476 t
ORIGIN      
        1 ggataacgat ggagtatgat caggcagcag gcagtagtga tgagaataac agccattatg
       61 gcttataaca ttgcagatat gtgatttgtg ggcattggtt ttgcccgtag agtaaaacat
      121 tataaaacca taaagtcctg agatatcatg gcatacaatt tacataaccg cagctttctg
      181 aaactgcttg atttcacacc ggaagagata caatttcttc tcgatctttc atttgacctc
      241 aagaaggcga aatatgccgg aaccgaacag atgaggctca aaggaaaaaa aatagctctc
      301 atttttgaga aaacatctac ccgtacccgc tgcgcctttg agtcggccgc attcgatcag
      361 ggtgcacatg taacctacct gggtccttca ggatcacaga ttggccacaa ggaatcaatg
      421 aaggatacgg caagggtact ggggcgcatg tacgatggta ttgaataccg cggttatgga
      481 cagaagattg tcgaggaact tgctgcacat gccggcgttc cggtatggaa cggcctcacc
      541 gatgagttcc atcccacaca ggttctggct gatttcatga ccatgctgga gcacagcgac
      601 aaacccctta acaagatcag cttttgttat ctcggtgatg cccggaacaa tatgggaaac
      661 tcgctgatgg caggggctgc aaaaatggga atggatttca ggtcagctgc tcccagggca
      721 tgccagcctg cagatgacct cgtgaagaaa tgccgtgaaa tagccgctga aacaggtgcg
      781 aagattaccc tgactgagga tgtggctgag gctgtcagag gtgttgattt tctttacact
      841 gacgtatggg tatccatggg tgagcctgac tccgtgtggg aggagagaat aggtctgctg
      901 aagccatacc aggtcaacag tgaggtaata aggctgacag gaaaccccaa tgtcaagttc
      961 cttcactgcc ttccggcctt ccataacagg gagacgaagg tgggcgaaga gatatacaag
     1021 aaatacggtc ttgacggaat ggaggtgacg gaggaggtat tcgaatcaag tcactcaata
     1081 gtatttgacg aagccgaaaa ccgcatgcat actatcaaag cggtgatggt ggctactctg
     1141 ggtgagtaga aaggcagcag tgccgggggc aagcagaaaa gcagcagtgc cgggggcaag
     1201 cagaaaggca gcagtgccgg ggcatgcaga aagcagcagt gccggattgc agaccggcat
     1261 tctatatcac cagcgccctg atgaaataga atgtcatcag cagcgctgct gccagcttga
     1321 ggcctacccc ggtgaggaag cccaggaggc tgccgaatcc tgcttttagg gcatacctga
     1381 tgtcatcctt gaatatcagc tctccaacca cggcccctat aaagggacca atgattattc
     1441 ctacaggccc aagaaagagc ccgatgatca acccgacggt ggcaccacgc atcccatatt
     1501 tggagccacc gaattgccgc gtaccccaga ttggcaccac gtagtctatc acggtaacca
     1561 caattgcaac cacgccgaga atgatgaaca ggtttttgct tatttcagcg aaactggtaa
     1621 agtgaaggac aaccaggccg agataggtca gcggcggacc aggcaggacg gggaggaggc
     1681 agcctgcaat gccaagtatc ataagaatga tggctaacgc cagaagcaga taatccatat
     1741 gaagtcgggt ttcagtaaat taagcagggc acggttacct tgtacccagt tcgtcaaaat
     1801 cgacatccga gaattccgtt gctgccactc cggttacagt ctccccgcaa tcttccgtct
     1861 caggtgaagc gtgggtggaa actgcagtac cattgtctgc aaaggtgata gcctcgtgaa
     1921 gcccctcaga gaacttctca aaatcctcct tatagagaaa aattttgtgt ttctcataaa
     1981 acatcttgcc ctctttcccc ggtctctttt tgctctcggt gattgtcaga tagagatcct
     2041 catgacgcgt tgacttcaca tcaaaaaagt atgtcctctt acctgccctg acaacttttg
     2101 taaacacctc ctctt
//
LOCUS       DAOPBK010000270         2048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_42968_length_2048_cov_4.450075, whole genome shotgun sequence.
ACCESSION   DAOPBK010000270 DAOPBK010000000
VERSION     DAOPBK010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42968_length_2048_cov_4.450075"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..868
                     /locus_tag="PLA17_11070"
     CDS             107..868
                     /locus_tag="PLA17_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HPK85993.1"
                     /translation="MNDLLFRVTDFLRDNRQSVLCIVTAVTGSSPAKAGAKMIVFDDG
                     AHEGTVGGGAVEQQVISDAVEIIKEQTAQSKKYNLHNDLSMACGGMMTVYFEPLKKPA
                     RLYIFGAGHIGRQLAGFTPDFGFETFLIDWREDIFEKSENLRYTQIRKPYLEAVEEIT
                     FDENTYCVIVTPGHEMDEEVLAAVGRKPTAYTGMIGSRNKIATLRKRFLDEKILTEDE
                     LNRVDMPIGIRFNAITPAEISISILARLIDVKNTR"
     gene            865..1680
                     /locus_tag="PLA17_11075"
     CDS             865..1680
                     /locus_tag="PLA17_11075"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPK85994.1"
                     /translation="MIQDKIYKFLNLNYKDQVEGLKIDEVCFGSHLTAVRLTDGSYGV
                     ASTLSGKQRHCTKQNRDFGDFSPLNIRNQSVKSLFETQRLSNAIDTLRIAVLNAISSR
                     ILSSGRYRVIEDKDPIELIDLKEAGTVTIVGAFHSYIEAVAAAGSRLHVLELNRDALN
                     EECMKYYVPAEDYAKVIPESDVVIITGMTLVNNTIDGLLSVIKPETKVIVTGPSSSII
                     PDILFENRVSIIGATKIEKPELLFQLVSEFGTGYHLFRYCARKVCIINEYLQA"
BASE COUNT          582 a          420 c          498 g          548 t
ORIGIN      
        1 tgaagacctt cctgaatgac gaaccaattg caatatcaca gggaagatat gatctcggta
       61 tcggatatga aaatttttat aatctttaat gtctaaaaca gacaggatga atgacctatt
      121 attcagagta acagatttcc tgagagacaa ccgtcagtca gtcttatgta ttgtcactgc
      181 cgtaaccggc tcatcacccg ccaaagccgg agcgaagatg atagtgtttg atgacggagc
      241 ccatgaaggc actgtaggag gaggtgccgt ggagcaacag gtgatcagtg atgccgtgga
      301 aatcataaag gaacaaactg ctcagtcaaa gaagtacaat cttcataatg atctgtcaat
      361 ggcctgcggg ggcatgatga cagtttactt cgaacccctt aagaaaccgg caaggctcta
      421 catcttcggg gcagggcata ttggcaggca actcgccgga ttcacgcctg actttggatt
      481 tgaaaccttc cttattgatt ggcgtgagga tatcttcgag aaaagtgaaa acctcaggta
      541 cacccagata cgcaaaccct atctcgaagc tgttgaggag ataacttttg atgagaatac
      601 ctattgcgtc attgtaactc ccggacatga gatggatgaa gaagttctgg ctgccgttgg
      661 caggaagcct acggcctata cgggaatgat tggcagcagg aataaaattg caacactgcg
      721 aaagcggttt ctggatgaaa agattcttac cgaagatgaa ctaaaccggg ttgacatgcc
      781 tattggcatc agattcaatg ctattacacc ggcagagata tccatcagta tccttgccag
      841 acttattgat gttaaaaaca cgcgatgata caggataaga tatataaatt ccttaatctg
      901 aactataaag atcaggttga gggtttgaag attgatgagg tttgtttcgg ttcccatctg
      961 actgccgtca ggcttacaga cggaagctat ggtgttgcat ccactttgtc aggcaagcaa
     1021 cgtcattgta caaagcaaaa ccgtgatttc ggggatttct ccccgttaaa tatcagaaat
     1081 cagagcgtta agtcactatt cgagacacag aggctgtcga atgccataga tactctcaga
     1141 atagcggtat tgaacgcgat atcgtcaaga atactgtcaa gtggcagata cagggtcatt
     1201 gaggacaaag acccgattga gttgattgac ctgaaggaag ccggaacggt aactattgtc
     1261 ggggcgttcc attcatatat tgaagcagtt gctgctgccg gcagcaggtt acatgttctt
     1321 gaactgaaca gggatgcgct gaatgaagag tgcatgaagt attatgtccc tgctgaggat
     1381 tatgcaaaag ttatcccgga atcagatgtg gtgattatca ccggcatgac acttgtgaac
     1441 aacactattg acggtctgct ctcagtaata aaacccgaga cgaaggtgat tgtcacaggg
     1501 ccatccagca gcattatacc cgatattctt tttgaaaata gagtcagtat tattggagcc
     1561 actaagattg aaaagcctga gctgcttttc cagttagtca gcgagtttgg cacaggatac
     1621 cacctgttca gatattgtgc ccgcaaagtc tgtataatta acgagtacct gcaggcttag
     1681 cggagggtat ctggcataga cataatcctg gctgcttcct tgacaccggg gcaatccggg
     1741 tgcttcacaa gcatcagcat aatccgggct gattcactgg tatagagata atcctgactg
     1801 ctacaaattg acccggggta tttgctataa tcattacgtg tgtttgtgtt tctgaggagt
     1861 gtgtcagcca gtatagttca acttattaat ccggggtatt tgctataata atccagtgtt
     1921 cgtgttcctg aggagtgtgt gatccggcac agctcaactg ttcacctccc caggtatttt
     1981 ttgtaatcat cacgtgtgtt tatatttctc aagggcgtgt catcagccat ttccatgtta
     2041 attctggg
//
LOCUS       DAOPBK010000271         2040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_43225_length_2040_cov_4.864987, whole genome shotgun sequence.
ACCESSION   DAOPBK010000271 DAOPBK010000000
VERSION     DAOPBK010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43225_length_2040_cov_4.864987"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2040)
                     /locus_tag="PLA17_11080"
     CDS             complement(<1..>2040)
                     /locus_tag="PLA17_11080"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPK85995.1"
                     /translation="AVTLRIDPAISAGRPATPVFLVRASLVIDGYSPSRPVTKSFIFL
                     DKVLTQGHPGGEWPSSPVNGQVIDLGMDPGVVNDPRYSQQMKASLTDIPSISLVTDLA
                     DMFGASRGIYVNAYQHGEEWERQCSLELINPDNTIGFRVNAGVRIRGGASRSPDNPKH
                     AFRLFFRKEYGPGKLLYPLFGDEGAAEFDKVDLRTEQNYSWSKEGDTHNTFLRDIFSR
                     DTQRDMGQPYTRGRYYHLYLNGMYWGLFQTEERPDADYAETYFGDGDDDYDVIKVSVE
                     TWPYLNEATDGTMESWQELYNRCNRGFASNSDYFALEGKDQNGNPVKNTRVWVDIDNL
                     IDYMLVIFYTGNFDAPVSAFYGNAMANNYFAILNRKNKGEGFIFIAHDSEHSMFVRPY
                     SLSSGINENRVSISNPAMTVSGISNFQPQWLHHKLTSNAEYRLRFADRAYKRFTAGGV
                     LSPGKCINRFNARKAEVEMAIIAESARWGDAKTPQPRNKIDHWLPEVNDIVNNFFPDR
                     SDIVMSQLRSANLYPSLEPVVVKESGLTIWEEKIVYTSLTITLENPNNRGQIYYTTDG
                     SDPRQPGGTVSSKAVQAQNGSSLGVSGSMIFRSRILDGQTWSALREVRFSAFMEDYSN
                     LKVTELHYHPADLIRDRDTIDGKDLEFIELKNTGKGAMNISGITLDTAVYYRVPD"
BASE COUNT          446 a          531 c          518 g          545 t
ORIGIN      
        1 cgtcgggaac cctgtaatat actgcagtat caagtgtaat gccggatatg ttcatcgcac
       61 ccttgcccgt attcttcaac tcaatgaatt caaggtcctt gccgtcaatg gtatctctat
      121 cccttatcag gtcagcgggg tggtaatgca actcagtcac cttgaggttg ctgtaatcct
      181 ccatgaaggc gctgaacctc acctccctga gtgcactcca ggtctgtccg tcaagtatgc
      241 gtgacctgaa gatcattgaa cctgaaactc ccagtgatga gccgttttgc gcctgaactg
      301 cctttgatga gactgtccct cccggctggc gggggtcact gccgtcagtg gtgtagtaaa
      361 tctgtcccct gttgttggga ttctcaagcg ttattgtcaa tgaagtgtag acaatcttct
      421 cctcccatat tgtaagcccc gactctttta caaccacagg ttccagtgac gggtaaaggt
      481 ttgcagacct gagctggctc ataacgatgt ctgatctgtc agggaagaaa ttgttcacaa
      541 tgtcatttac ctccgggagc cagtggtcaa tcttgtttct gggctgtggg gtttttgcgt
      601 cgccccaacg ggcggattca gctataatgg ccatctctac ctccgccttt ctggcattga
      661 acctgttgat gcattttccc ggcgagagga ctcctccggc tgtaaaacgc ttataggccc
      721 tgtcagcaaa gcgaagcctg tattcggcat ttgatgtcag tttgtggtgc agccactggg
      781 gctggaagtt gctgattccc gacacagtca ttgccggatt gcttattgac accctgtttt
      841 cattgatgcc gctgctcagc gaataggggc gaacgaacat ggagtgttcg gaatcatgtg
      901 cgatgaatat aaatccttca cccttgtttt tccggttcag gatggcaaag tagttgttgg
      961 ccatcgcatt gccgtaaaaa gcagagacag gggcgtcaaa attcccggta tagaagatga
     1021 ccagcatgta atcaatcagg ttgtctatat caacccatac cctggtgttt ttaaccggat
     1081 taccgttctg gtccttacct tccagggcga aatagtcact gttggatgca aagccacggt
     1141 tgcaccggtt gtagagctcc tgccatgatt ccatggtacc atcggttgcc tcattcaggt
     1201 aaggccatgt ttcgacagat accttgatga cgtcatagtc gtcgtctccg tctccgaaat
     1261 aggtttcggc atagtctgca tcgggccgct cttcggtctg gaagagaccc cagtacatgc
     1321 cgttgaggta gaggtgataa tatcttcccc ttgtataagg ctgccccatg tcgcgctgag
     1381 tatcccttga aaagatatct ctcagaaagg tattatgagt atctccctcc ttgctccatg
     1441 aatagttctg ttctgttctc aggtcaacct tatcgaactc agcggctcct tcatcgccga
     1501 agagcggata gaggagcttg cccgggccgt actcctttct gaagaagagc cggaaggcgt
     1561 gtttcggatt gtcaggagat ctgcttgcac ctcctcgtat gcgtaccccc gcgtttaccc
     1621 ggaaccctat ggtattgtca ggattgatga gttccagcga acactgtctt tcccattcct
     1681 cgccatgctg gtaagcattg acgtatatgc cccgtgaagc gccgaacata tcagcaagat
     1741 cagtcacgag ggatatcgaa ggtatatccg tcagacttgc cttcatctgc tgactgtatc
     1801 tggggtcatt caccacgccg gggtccattc cgaggtcaat tacctgccca ttgaccggtg
     1861 atgagggcca ctctcccccg ggatgaccct gggtcagtac cttgtcaaga aaaatgaatg
     1921 actttgtaac gggtctggaa ggcgaatagc cgtcaatgac cagcgaggca cgcaccagga
     1981 acaccggcgt ggcagggcgg cctgccgata tggccgggtc aattctcagc gtgacagccg
//
LOCUS       DAOPBK010000272         2009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_44125_length_2009_cov_4.659161, whole genome shotgun sequence.
ACCESSION   DAOPBK010000272 DAOPBK010000000
VERSION     DAOPBK010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44125_length_2009_cov_4.659161"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLA17_11085"
     CDS             complement(<1..764)
                     /locus_tag="PLA17_11085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPK85996.1"
                     /translation="MRLLKTILTLACLSCAPFILGQEPDTVLTLLFTGDIMGHDGQIA
                     SARNDSTGTYEYDSVFRYITPFISSADVATGNLEVTLGGPPYKGYPAFSSPDELAVAC
                     RNAGFDILVTANNHSADRGPKGIFRTLRVLDSLGIRHTGTWISPEERDIISPLMICHE
                     SMRIALLAYTYGTNGIVVPPPATVAYIDTIRAATDIRRAELLGADLTIIFIHWGIEYD
                     TIPSAEQKKTAAALRRAGADIIIGSHPHVVQPVAAE"
     gene            838..1428
                     /locus_tag="PLA17_11090"
     CDS             838..1428
                     /locus_tag="PLA17_11090"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPK85997.1"
                     /translation="MEHLNTEIVNYLLLPLFIFLARIFDVSLGTLRIIFVTKGMRIIA
                     PLVGFFEVLIWLLAISRIMQDLDNWVSYIAYAGGFATGNFVGMYLEERLAIGHEMIRV
                     ITRKDATSLIEKLRDMGYGVTSVKAEGIEGEVAIIYIIARRRMIGEVLDKINYFNPRA
                     LYTVESIKYVNKEIFHKSEEERQKSWLAGRLIRKGM"
     gene            complement(1435..1848)
                     /locus_tag="PLA17_11095"
     CDS             complement(1435..1848)
                     /locus_tag="PLA17_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK85998.1"
                     /translation="MKPSLTVIVLITAMLTSCNLEPGPMSYTNVAIPFEEYQVPETGI
                     IDQPVNIYVSARADNGCWSNIRFLLKQNDDREFDAWAIADFSSDGECPAVEVSADSTL
                     TFTPTRTGDHVISFWMTHNISIRDTVVVVAAPDRK"
BASE COUNT          474 a          478 c          526 g          531 t
ORIGIN      
        1 ctttcagccg ccaccggttg caccacgtga gggtgggagc cgatgatgat atcagcgcct
       61 gccctccgca gcgctgctgc cgtcttcttc tgttcggctg aagggatggt gtcatattca
      121 atgccccagt gaatgaaaat aattgtcagg tctgccccca gcaattcagc tctcctgata
      181 tcagtggcag cacgtatggt gtcaatatat gcgaccgtag ccggtggcgg caccactatc
      241 ccgtttgtgc cgtaggtgta tgccagcagg gctattctca ttgattcatg gcatatcatc
      301 aggggagaaa tgatatcacg ttcctccgga ctgatccatg tgccggtatg tcttatccca
      361 aggctgtcaa gcaccctcag ggtgcgaaag attcctttcg gtcccctgtc ggctgaatga
      421 ttgttcgcgg tcaccaggat atcgaacccg gcattgcggc atgccactgc cagctcatcg
      481 ggcgagctga aggccgggta acccttgtag ggaggccctc ccaacgttac ctccagattg
      541 cctgtggcaa catctgcgga tgagatgaag ggagtaatgt atctgaagac actgtcatat
      601 tcatatgtgc ccgtggagtc atttcgcgct gaggctatct gcccgtcatg ccccataatg
      661 tcaccggtga agagcagcgt aagaaccgta tccggttcct gccccaggat gaaaggggcg
      721 cagctaaggc atgccaatgt caatatggtt ttcaataatc tcatcgacac atggatttag
      781 gccaccgggt ccaaaaatag tcatatctta gcaagttcaa aaatgtttgt tattgtcatg
      841 gaacatctta atacagaaat tgttaattat ctcctgctcc cgctcttcat ttttctggcc
      901 aggatttttg acgtttcgct cggtactctc agaatcatct tcgtcaccaa ggggatgagg
      961 attatagctc cgttggtagg ctttttcgag gtgcttatct ggcttctggc aatcagcagg
     1021 atcatgcagg accttgataa ctgggttagt tatattgctt atgcaggtgg attcgccaca
     1081 ggtaatttcg tggggatgta cctcgaggag cgtcttgcca taggtcatga gatgataagg
     1141 gttatcacac gcaaggatgc caccagcctg attgagaaac tcagggacat ggggtacggt
     1201 gtgacatcgg taaaggccga gggaatagag ggtgaggttg cgataatcta tatcattgca
     1261 cgcaggagaa tgatcggcga ggtgcttgac aaaatcaact atttcaaccc gcgcgcactt
     1321 tatacggtgg aatcaatcaa gtatgtcaac aaagagatct tccacaagtc agaggaggag
     1381 cgtcagaaga gctggcttgc cggcaggttg atacgcaagg gtatgtagcc ttttttactt
     1441 tctgtccggt gcggcgacca caaccaccgt gtccctgatg ctgatgttgt gagtcatcca
     1501 gaaagaaatg acatgatctc ccgtccgggt gggagtgaag gtgagggttg aatcagcaga
     1561 aacttccaca gcggggcact ctccgtctga tgagaagtct gcaatggccc aggcatcgaa
     1621 ctcacggtcg tcattctgtt ttagcaggaa gcggatgttt gaccagcatc cgttatctgc
     1681 cctggcactg acgtagatat taaccggctg atcgattatg cctgtctcag gcacctgata
     1741 ctcctcaaac ggaatcgcca catttgtata cgacatgggc ccgggttcaa ggttgcatga
     1801 tgtcagcatg gctgtgatca gcactattac tgtaagtgaa ggtttcatct ttctgtttgt
     1861 tattctccgg tttgctgtta cagcgcttca atgctacaat aaccatacca actcattcgg
     1921 tatgatatag tgcgaaggtg cttatataag gggcagcccg tgatgagact attgttagtt
     1981 ttatctcgtc tgccttcacc tcaggaaac
//
LOCUS       DAOPBK010000273         1961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_45626_length_1961_cov_3.896118, whole genome shotgun sequence.
ACCESSION   DAOPBK010000273 DAOPBK010000000
VERSION     DAOPBK010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45626_length_1961_cov_3.896118"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PLA17_11100"
     CDS             <1..1081
                     /locus_tag="PLA17_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/glucose cotransporter"
                     /protein_id="HPK85999.1"
                     /translation="LAGSIAVLIAGLIKVGGWDELMHICRAVPVNEYGDTMTQLIRSP
                     KDAEFPWTGVLLGSAIIGFWYWCTDQYIVQRVLSGRDQTQARRGAIFGGALKLTPVFI
                     FLIPGMIAYALNQQGVITLASSDSAFSTLVKELLPMGFKGLVVGGILAALMSSLASLF
                     NSSATLFTIDFYQKYKPNASEAQLVRVGRIATVTVVILGVLWIPVMKGIGKVLYAYLQ
                     DVQSLLAPGIAAAFLLGILSKKITPAAGLTGLLTGFSIGMLRLIFMVFKGSLNPDGTI
                     YQIFVGTNWLHYEIINFAIVIITMIIVSYFTPKIDERRIVGLTLGSATAEQKAVTRAS
                     WNKWDVITSGLIIAVIIAFYAYFWN"
     gene            1204..>1961
                     /locus_tag="PLA17_11105"
     CDS             1204..>1961
                     /locus_tag="PLA17_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HPK86000.1"
                     /translation="MLKKGNFELWFITGSQHLYGEETLKQVADDSKAIAAALDKSDII
                     PLKVVFKPVLTTASAITSICMEANNSPACAGLIAWMHTFSPAKMWIAGLKRLSKPMLH
                     FHTQFNRDIPWNEIDMDFMNLNQSAHGDREFGFICSRLRMERKVVVGHWQDMDVLRSI
                     GAWARAAMGALEARSLRVARFGDNMRDVAVTEGNKVSAQLMLDYEVQGYGIGDLAVAV
                     NKVTDKQISALVKQYSDEYKASAKVIAAPAVKEAA"
BASE COUNT          475 a          456 c          504 g          526 t
ORIGIN      
        1 gctcgccggt tcaattgctg tcctgattgc cggtctgatc aaggttggag gatgggatga
       61 actgatgcac atttgccgtg ccgtaccggt taacgaatac ggagatacca tgacccagct
      121 catacggtcg cccaaggatg ccgagtttcc atggactgga gtactgctag gctcggccat
      181 aatagggttc tggtactggt gtactgacca gtatattgtt cagcgcgtgc tttcaggacg
      241 tgaccagact caggcccgcc gtggtgccat cttcggaggt gctctcaagc tgacccctgt
      301 ctttatattc cttattccgg gaatgatagc ctatgccctt aatcagcagg gtgttattac
      361 gcttgccagt tctgattcag ctttctcaac tcttgttaaa gagttgctcc cgatgggatt
      421 caagggcctg gtggtaggag gtatccttgc tgccctgatg agttcactcg catcgttgtt
      481 caactcttcg gcgacactct tcaccattga cttttaccag aaatacaaac ccaatgcgtc
      541 tgaagcacag cttgtaaggg taggccgtat tgccaccgtc acggtggtca tccttggcgt
      601 cctctggata cccgtgatga aaggaatcgg taaagtgctc tatgcctacc tgcaggacgt
      661 tcagtcactt cttgccccgg gaattgcagc agcatttctc cttggtattc tgtcaaagaa
      721 gattactccg gcagccggtc ttacgggtct gctgacagga ttctcgatag gaatgctccg
      781 tctcatattc atggtgttca agggtagcct caatcctgac ggcaccattt accagatatt
      841 cgttggcacg aactggctgc actatgagat catcaacttc gctattgtga tcatcacaat
      901 gattatagtc agttacttta caccgaagat tgacgagcgg cgaattgtcg ggcttaccct
      961 gggttcggca acggctgaac agaaggctgt taccagggca agctggaaca agtgggatgt
     1021 tattacttcc ggtcttatca tcgccgtaat aatagcattc tatgcctatt tctggaatta
     1081 acatgattta tttatctgac atttaatcaa gatggaaccc acatgtatca aatacataaa
     1141 caggtattaa tataatttgc tgtcatgcgt gttccatcat accttcaaaa tcaaaattat
     1201 actatgttga aaaaaggaaa ttttgaactt tggttcatca cgggaagcca gcatctttat
     1261 ggagaagaga cgctgaagca ggtggctgat gattcaaaag ccattgctgc tgcgcttgac
     1321 aagtcggata taatcccgtt aaaggttgtt ttcaaaccgg tactgaccac tgccagtgca
     1381 atcacctcga tatgcatgga ggccaacaat tctccagcat gtgcggggct gatagcctgg
     1441 atgcacacct tctcgcctgc aaagatgtgg atcgccggtc tgaagaggct gagcaaaccc
     1501 atgcttcact ttcatactca gttcaatcgc gatattccgt ggaatgagat agatatggat
     1561 ttcatgaacc tgaaccagtc agctcacggt gaccgtgagt ttgggttcat ctgttcccgt
     1621 ctccggatgg agcgcaaggt ggttgtaggg cactggcagg atatggatgt gcttcgctcc
     1681 atcggagcat gggctcgtgc agccatgggc gcccttgagg cacgttcgct cagggttgcc
     1741 cgtttcggtg ataatatgag ggatgttgcc gttaccgaag gcaataaggt gtcagcacag
     1801 ttaatgctcg attatgaggt gcagggatat ggtatcggag atctcgcggt tgcagtaaat
     1861 aaggtgacag acaagcagat cagcgccctg gtgaagcaat acagcgatga gtataaggct
     1921 tccgcgaagg taattgcagc accggcagtt aaagaggcag c
//
LOCUS       DAOPBK010000274         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_46630_length_1932_cov_3.910495, whole genome shotgun sequence.
ACCESSION   DAOPBK010000274 DAOPBK010000000
VERSION     DAOPBK010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46630_length_1932_cov_3.910495"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PLA17_11110"
     CDS             <1..676
                     /locus_tag="PLA17_11110"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPK86001.1"
                     /translation="TSGVSNALVADYASIFASPHSTKESVFSLPHTTANNAGTQNHLA
                     HYFDPESGESYYLVTGDGSLYNAMDDTDARKIMIRVNASDGQPYISKYTDTGTRSDYV
                     PVIRWAEVLLNRAEGIVRDGGSVTQAAIDLLNAVRVRSFETGAYDIADFASADEFYTA
                     ILQERNIEFLGEGLRNMDLLRLGLTIPGKGSAGFGTVGAVPSTSQAYIWPVPDSERSY
                     NKLMTP"
     gene            complement(844..978)
                     /locus_tag="PLA17_11115"
     CDS             complement(844..978)
                     /locus_tag="PLA17_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK86002.1"
                     /translation="MMQRDFIIIGAMIMVLIIVYMIMRHRTDKIRSTRNNRTRFRRNQ
                     "
     gene            complement(989..1624)
                     /locus_tag="PLA17_11120"
     CDS             complement(989..1624)
                     /locus_tag="PLA17_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPK86003.1"
                     /translation="MKLTSSIFFLVLLPLTVMAQRDPDAVAVLSEFSKKAASAPSVKI
                     DFNILVYDAKEDQETETEGTVVISGDNYRLTLPDNLIMADGKTVWNYMPDVKEVTVTE
                     PDPADDSFMSKPSMLFSMYETGYKVRLLEQNPTEWIIDLYPEDVRLNLVRIRLAIGRN
                     LHDLRSAEYKTKDGITLTLKVARYDLTFRPPAGYFAFNPADHKGVEVIDMR"
     gene            complement(1626..>1932)
                     /locus_tag="PLA17_11125"
     CDS             complement(1626..>1932)
                     /locus_tag="PLA17_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPK86004.1"
                     /translation="FHLPEYVGDDDIGNSNSEVDLKKRDPLFEEAARLVVQHQQGSTS
                     LIQRRMQLGYNRAGRIIDQLEAAGIVGPFEGSKAREVIVQDPQTLEQILKRLEQENL"
BASE COUNT          467 a          486 c          455 g          524 t
ORIGIN      
        1 cacttcaggt gtcagcaatg cacttgttgc tgactacgca agcatctttg catcacctca
       61 ttcaaccaaa gagtctgttt tctcattgcc tcatacgacg gctaacaacg ccggaacaca
      121 gaaccacctg gctcactatt ttgatccgga atccggagag tcttactacc ttgtgacagg
      181 cgatggctca ctctataatg ctatggatga taccgatgca aggaagatca tgatcagggt
      241 taatgcaagt gatggacaac catatatttc caaatatact gatacgggta caaggtctga
      301 ctatgtgccg gtgatcagat gggctgaggt gttgcttaat cgtgctgaag gaatcgttcg
      361 cgacgggggc agcgtcacac aggcagcaat tgacctgctc aatgctgtaa gagtacgctc
      421 ctttgagacc ggagcatacg atattgctga ttttgcctct gctgatgaat tctataccgc
      481 tattttgcag gagagaaata ttgagtttct tggagaaggt ctccgcaaca tggacctttt
      541 gagactgggg ttgacaatac ccggtaaagg aagtgcagga ttcggtacgg ttggagctgt
      601 tccttccaca tcacaggcat atatctggcc tgttcctgac agtgaaagaa gctataacaa
      661 gctgatgaca ccataataac agattacgtg aataaacggg agtgccttcc cttcaagggg
      721 cactcacccg tttatcagat tataatttgc cgctcccgga agggagcggc ttttattatg
      781 tgtgcccggc atggacaata gctgcagggt ggaaggcaca actgcgcact tctcattcta
      841 ttgtcattgg ttccgcctga acctggtccg gttattgcgg gttgacctga tcttgtcagt
      901 acggtgcctc atgatcatgt aaacaatgat cagcaccatg atcatggcac ctattattat
      961 gaaatcacgc tgcatcatat agctgtcttt accgcatgtc aatcacctca acccctttgt
     1021 gatcagcagg attgaaggca aaatacccgg ccggaggccg gaacgtaagg tcatacctgg
     1081 caaccttcag ggtaagggta ataccatctt tggtcttgta ctctgctgac cggaggtcat
     1141 gaagattcct gccaatggcc agccttatac gcaccaggtt cagcctgaca tcttcagggt
     1201 agagatcgat gatccactca gtgggattct gttccagcag cctgacctta tatcctgtct
     1261 cgtacatcga gaagagcatc gaaggcttcg acatgaacga gtcatcagcc ggatccggtt
     1321 ccgttactgt tacctctttt acatcgggca tataattcca tactgtcttg ccgtcggcca
     1381 ttatcaggtt gtcaggcagc gtcagcctgt agttgtcgcc gctgatcacc acagtgcctt
     1441 cagtctcagt ctcctgatcc tcctttgcat catataccag tatgttgaaa tctatcttta
     1501 ccgaaggggc agaggctgcc tttttgctga attccgaaag gacagctact gcgtcagggt
     1561 ctctctgtgc catgacagtc agaggcagaa gcaccagaaa aaatattgag cttgtcagtt
     1621 tcattttaaa ggttttcctg ctcaagtctc ttcaaaatct gttccaatgt ttgcggatcc
     1681 tgaacaatca cctcgcgtgc cttgcttcct tcgaagggcc cgactatgcc ggcagcctca
     1741 agttggtcaa taattctgcc cgcacggttg tatcccagtt gcattcttct ctggatcaga
     1801 gaggtagatc cctgctgatg ctgtaccacc agcctggctg cctcctcaaa cagcgggtca
     1861 cgctttttca ggtcaacctc gctgttgctg tttccaatat catcatcgcc cacgtattca
     1921 ggcagatgga ac
//
LOCUS       DAOPBK010000275         1867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_48972_length_1867_cov_4.242826, whole genome shotgun sequence.
ACCESSION   DAOPBK010000275 DAOPBK010000000
VERSION     DAOPBK010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48972_length_1867_cov_4.242826"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="PLA17_11130"
     CDS             <1..1255
                     /locus_tag="PLA17_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HPK86005.1"
                     /translation="HVTEILKLVNSCPVFLDHNRGAWHYGISHEHDGVIGENLARLGD
                     IVFCSINHRLGPMGFSDFSAVGGEKYADSGNSGALDLVAALEWIRDNISNFGGDPANV
                     TIMGQSGGGGKVVTTAAMTPAKGLFCKVVSLSAADFELRDKEMTRKVGYYILKEAGLQ
                     PSQIDKLQEMPWRIYYDIAIRAAEKYIKETGTTLSPMKVFQPQVDGRNIEYPTFFPEA
                     SPVMAEVPMIISSTTNESSNAWKDSSLESMTLDGMKKEMTRNYGEKAGEIADAYLKAF
                     PSKKPYELLSIATNTFRQRAVALANAKSRQSPPVFMALFGWQPPLFNNRLMAFHTLDQ
                     CFWFANTDLMITHTGGGARPRALSEKMAGSLIQFMKTGDPNGGGLSHWPVYTSEKGEL
                     MFLDDICEVQYDPDREARKTLPPII"
     gene            complement(1688..>1867)
                     /locus_tag="PLA17_11135"
     CDS             complement(1688..>1867)
                     /locus_tag="PLA17_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK86006.1"
                     /translation="VSAKTLYKLVIPWRAILIVVLGLGFNHVIIILHRICRDIITCLQ
                     DIGYKVVLNAIYEML"
BASE COUNT          544 a          381 c          464 g          478 t
ORIGIN      
        1 tcatgtgaca gaaattttaa agttagtaaa ttcctgtcca gtttttctag accataacag
       61 aggcgcctgg cattatggca tcagtcatga gcatgacggg gttattggtg agaaccttgc
      121 caggttaggt gatatcgtct tctgttcgat aaaccacagg ctcgggccca tgggattctc
      181 tgacttttct gctgtggggg gagaaaaata tgcagattcg gggaattccg gggccctaga
      241 cctggtggca gctcttgaat ggatacgtga taatatatca aactttggag gtgatccggc
      301 aaatgttact atcatgggtc agtctggtgg cggaggtaag gttgtgacca ctgcagctat
      361 gacccctgca aaaggactct tttgcaaggt tgtatctctc agtgcggctg attttgagct
      421 gagagacaag gagatgactc gcaaagtcgg ttattatatc ttgaaggaag ccggattaca
      481 gccttcgcag atagacaaac ttcaggagat gccttggagg atctattatg acatcgccat
      541 tcgggctgct gaaaagtata taaaagagac tggtactact ctctccccga tgaaagtgtt
      601 ccagcctcag gttgacggca ggaatataga atacccgacc ttttttcctg aagcctcacc
      661 ggtaatggct gaagtgccga tgattatcag ctcaactacc aatgagagct caaatgcctg
      721 gaaggactct tcacttgaat cgatgactct cgatggcatg aaaaaggaga tgaccagaaa
      781 ttacggggaa aaggcaggtg aaatcgcaga tgcctatcta aaggctttcc ccagcaaaaa
      841 gccctatgaa ctactgtcaa tagccacgaa tacgttcaga caacgggcag tggcacttgc
      901 taacgccaag tcaaggcagt cgcctcccgt atttatggca ttgtttggct ggcaacctcc
      961 attgtttaat aacaggctga tggcttttca tactcttgat cagtgtttct ggtttgcaaa
     1021 tacggatctt atgattacac ataccggtgg aggtgcaagg cccagggcac tctctgagaa
     1081 aatggctggg tcattgattc aattcatgaa aacgggagac ccaaacgggg gtggattgtc
     1141 tcattggcca gtatatacca gtgaaaaggg ggagctgatg tttcttgatg atatttgtga
     1201 agtacagtat gaccctgata gggaagcgag aaaaacctta ccgccgataa tatgagggca
     1261 gatctatctt gccagccttg agatctgact tataaattgc ttgacaaagc ttgtgagaac
     1321 aatgaaaaca aacaggagaa tcttagtcag aataataggt accgaagatg cagggccggg
     1381 agtatctact actttgccgc tgtcatcctg ctcaggttca ggtagtggcg agactcctga
     1441 caacatcatt gctggaactg tcatcgacaa gagacatggt cataggtggt aaatcaggtc
     1501 tgtgctgaaa tcaaggacat ccgttacgac gtaccacaga accagatcag ctgagctttc
     1561 atggaaagtt gaaagaacac aacaaaacaa aagaattcac gacacagaaa tgtacgtgga
     1621 ggtgtgccag ttccgtgaca atataaatag gctggttgac cagatggatg aatgtgatta
     1681 catcttttca cagcatttca taaatagcgt tgagaacaac cttataacca atatcttgga
     1741 ggcatgtgat gatatccctg caaatccggt gcaaaattat gattacatga ttgaaaccta
     1801 ggccaagtac cacgatcagg atcgctctcc aaggtattac aagtttgtaa agagttttag
     1861 cgcttac
//
LOCUS       DAOPBK010000276         1865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_49042_length_1865_cov_4.944751, whole genome shotgun sequence.
ACCESSION   DAOPBK010000276 DAOPBK010000000
VERSION     DAOPBK010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49042_length_1865_cov_4.944751"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLA17_11140"
     CDS             <1..294
                     /locus_tag="PLA17_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPK86007.1"
                     /translation="FIMEKISFRDLVPAGTTEVRNTHINIGTGEEITIKDLSLLLKET
                     TGFGGELVFDTSKPDGTPRKFLDSSKLHSLGFRHATSLREGAAKMYQWYLDNL"
     gene            598..924
                     /locus_tag="PLA17_11145"
     CDS             598..924
                     /locus_tag="PLA17_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK86008.1"
                     /translation="MMKRAAVYLMTVVLAVMMAGCAASYIDSSQGRDGSSFEKAVIVG
                     SVRAEYIYIDRKYPNAQILSQMIVDNNGNPYDVVTIVPKGETKKDIYFDVSRFYRKKT
                     YADDLQ"
     gene            complement(1017..>1865)
                     /locus_tag="PLA17_11150"
     CDS             complement(1017..>1865)
                     /locus_tag="PLA17_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HPK86009.1"
                     /translation="EEKRGLGIYMTVLGFGMGNIKDDKMEIIADKGNGNYAYIDNIQE
                     ARRILVQEFGGTLFTIAKDVKFQIEFNPEYVKAYRLVGYENRLLADQDFNDDTKDAGE
                     MGAGHTVTALYEIIPAGSEETGLPTIDPLRYQSNGGSRKADGAHQTDEARQNGEIRKT
                     EESRQTGEAHQADGSRQTGEARKSLRNTHRELCNIKLRYKDPDALTSKLFSKTVGTEI
                     KKAGETTDRFRFSAAVAEFGMILRNSKYKGNATTADVISLATGARGADPDGYRAEFIR
                     LVRSAR"
BASE COUNT          426 a          437 c          504 g          498 t
ORIGIN      
        1 ttcatcatgg agaagatctc gttcagggac ctggttcctg ccggaacgac cgaggtgcgc
       61 aatacacata tcaatatcgg aaccggcgag gagataacaa ttaaggattt atctttgttg
      121 ctgaaagaga caacggggtt cgggggagag ctggtctttg acacaagcaa gcctgacggc
      181 acaccgagga aatttctcga ttcatcgaag ctgcacagcc tgggattcag gcatgccaca
      241 tcgctgagag agggcgccgc gaagatgtat caatggtatt tggacaattt atagttaatc
      301 ccccttcgct gaaagagggt gccgggagga tgtatcagtg gtatttggaa aatttatagt
      361 taatcccttg cgggggaata ttatatttgt aacgttgttt tcaatgctgt aaacgctacg
      421 ctgtaatatt cgccggaggc gataaagcat tgtaaaacac gattataagg aaaataatta
      481 ttcgcagaac atatggaagt gtaatatgaa acccgcatct gtcaaataca gaaacaccag
      541 tttaattggt tttgagtcat gtgggttcca tagtaccatt aaaatcagaa ttttatcatg
      601 atgaaaagag cagcagttta tttaatgacg gtggtgctgg cggttatgat ggccggatgt
      661 gcggcatcat acatcgattc atcgcagggc cgcgacggat cgtcgtttga aaaggcagtg
      721 atagtgggtt cggtcagggc cgaatatatc tacattgacc gcaagtaccc caatgcacag
      781 atactgagcc agatgattgt cgacaacaac ggcaatcctt atgatgtggt cactattgtg
      841 ccaaaaggcg aaacgaagaa ggatatttac ttcgacgttt cgcgctttta ccgcaagaaa
      901 acctatgcgg acgacctgca ataggctctg acgatcagtg gtaatactca ggatctgcaa
      961 taggctctga cgatcagtgg taatattctg actgagggcg ttgaagagta tgcggcttat
     1021 cttgctgacc ggacgaggcg gatgaactcc gcacggtagc cgtcggggtc agcgccccgt
     1081 gcgccggtgg ccagtgagat aacatcagca gtggtggcgt ttcccttata tttgctgtta
     1141 cgaagtatca ttccgaactc tgccacggca gctgagaacc tgaaccggtc ggtggtttca
     1201 ccggcctttt ttatttcagt gccaaccgtt ttgctgaaga gcttgctggt cagggcatcc
     1261 gggtccttgt aacggagctt gatgttgcag agctcgcggt gagtattcct gagcgacttg
     1321 cgtgcttcgc cggtctggcg tgatccgtca gcctggtgtg cttcgccggt ctggcgtgat
     1381 tcctcagtct tgcgtatttc accgttctgg cgtgcttcat cggtctggtg tgctccgtca
     1441 gccttgcgtg atccgccatt gctctggtac ctcagcgggt caattgtggg gaggccggtt
     1501 tcttcagatc ccgcagggat gatctcatac agggctgtca cggtgtgccc tgctcccatc
     1561 tcgccggcat cctttgtgtc gtcattgaaa tcctggtcgg ccagaagcct gttctcgtac
     1621 cccacgaggc ggtatgcctt gacatattcc gggttgaact caatctggaa ctttacatcc
     1681 ttcgctatgg tgaagagtgt gcctccgaac tcctgcacca gtatgcgccg tgcctcctga
     1741 atgttgtcta tataggcgta gtttccgttg cccttatcgg cgatgatctc catcttgtcg
     1801 tccttgatgt ttcccatccc gaatccgagg acagtcatgt agatgccgag gccgcgcttc
     1861 tcctc
//
LOCUS       DAOPBK010000277         1805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_51481_length_1805_cov_5.552571, whole genome shotgun sequence.
ACCESSION   DAOPBK010000277 DAOPBK010000000
VERSION     DAOPBK010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51481_length_1805_cov_5.552571"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLA17_11155"
     CDS             <1..624
                     /locus_tag="PLA17_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK86010.1"
                     /translation="FEYIRSFLTVAFSRFNPKTNPSVERLTPEEEPAMAKLLDEFYSG
                     YCFYTDEFSFYDHKYYVLRRNGEIVAGVGAIPACYRVVNVPGVWGWMMMKVLHRTPYF
                     RRLFQPEEFRYIILNAIYCRDGSEDLLPDLFEAVCAEEGYHTALTWLDDHSCLYETLR
                     TNRRMGTLNRLLNAKPGLVYALFSGLDPAETEKFYDSPAYISGFDFS"
     gene            685..>1805
                     /locus_tag="PLA17_11160"
     CDS             685..>1805
                     /locus_tag="PLA17_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPK86011.1"
                     /translation="MNVFHSFSIRSLLAVALFLFVGGQVSAQPYMPDRTSDDERMQWF
                     ADAKLGIFIHWGIYAVNGIDESWSFFNGLIGHDDYMKQLDGFTASSYDPNAWVRLIRE
                     SGARYAVLTSKHHDGVALWDTKLSRLNTVESTPAGRDLVKPFVKELRRAGLKAGLYYS
                     LLDWSHPDYPNLTRTVKRYSDDPERWQRFMQFNNGQMAELQKEFKPDLWWFDGDWEQS
                     AETWDAAGIRKTILERNPSAIINSRLAGYGDYGTPEQGLPSKRPADKYWELCMTMNDS
                     WGYQGNDRNYKSPSQVVRIFAGCIGMGGNMLLDIGPKADGTIPEEQVEVLREMGRWTS
                     KHKQAIYGTVAGVPDGLFAGPSTMSRDSTILYLFFPGNGG"
BASE COUNT          416 a          455 c          520 g          414 t
ORIGIN      
        1 tttgaatata tccgctcctt cctgacagtg gccttcagca ggttcaaccc gaagactaat
       61 ccttcggtgg agaggctcac cccggaggag gagcctgcca tggcaaaact cctggatgag
      121 ttctacagcg gttattgttt ttataccgac gagttttcgt tttatgatca taaatattat
      181 gtgcttcgcc ggaacggcga gatagtggcg ggcgtgggag ccatcccggc atgttacagg
      241 gtggtgaacg taccgggggt gtggggctgg atgatgatga aggtgctgca ccgtactcca
      301 tatttcagga gactgttcca gccggaggaa ttcaggtaca ttatcctgaa tgctatttat
      361 tgccgcgatg gcagcgaaga cctgctgccg gatctttttg aggccgtctg cgcagaggag
      421 ggctaccata ctgctctgac atggctcgat gatcactcat gtctctatga gacccttcgc
      481 accaacagga ggatgggcac tctcaacagg ctgctgaacg cgaagcccgg actggtgtat
      541 gccctctttt cggggcttga ccccgcggag accgagaagt tctatgactc cccggcatac
      601 atctcgggat ttgacttttc atagatagct gccccggcgc cccgggcagc gacattcttg
      661 taaaaaccag taaaacaata tattatgaac gtatttcatt ctttctcaat acggtcgcta
      721 ttggccgtgg ccctgttcct gtttgttggc ggacaggttt ctgcccagcc ctatatgccg
      781 gaccggacct ccgatgatga gcggatgcag tggttcgcgg atgcgaaact cggaatattc
      841 atccactggg gaatttatgc cgtcaacggg attgacgagt cgtggtcatt tttcaacgga
      901 ctcattggtc atgatgatta catgaagcag cttgacggct tcactgcctc gtcatatgac
      961 ccgaacgcct gggtcaggct gatcagggag agcggcgccc gctatgccgt gcttacctca
     1021 aagcatcatg acggggtggc cctctgggac acgaagctca gccggctcaa tacggttgag
     1081 agcacaccgg caggccgtga tctggtgaaa cctttcgtga aggagttgcg gcgcgcagga
     1141 ctgaaggcag gactttacta ttcactgctt gactggtcgc acccagacta tccgaacctt
     1201 acaagaacag ttaaaaggta ttctgatgac ccggaaaggt ggcagcggtt catgcagttc
     1261 aacaacgggc agatggccga gctgcagaag gagttcaagc ctgacctgtg gtggtttgac
     1321 ggcgactggg agcagagcgc cgagacatgg gatgcagcgg gtataagaaa aactattctg
     1381 gaacggaatc cctccgccat tatcaattcc aggctggcag ggtatggtga ttacggcact
     1441 cccgagcagg gactcccctc aaaacgtccg gccgacaaat actgggaact ctgcatgaca
     1501 atgaatgatt cctggggata tcaggggaat gacaggaact acaagagccc gtcgcaggtg
     1561 gtgcgtatct ttgccggttg catagggatg ggaggaaaca tgctccttga tatcggtccg
     1621 aaggccgacg gaactatccc ggaagaacag gttgaggtgc tcagggagat gggacgctgg
     1681 acctccaaac ataaacaggc aatatatggt actgtggcag gagtgcctga cggcctcttt
     1741 gcagggccct ccaccatgag ccgcgacagc accatactct atctcttctt tcccggtaac
     1801 ggcgg
//
LOCUS       DAOPBK010000278         1741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_54261_length_1741_cov_4.259786, whole genome shotgun sequence.
ACCESSION   DAOPBK010000278 DAOPBK010000000
VERSION     DAOPBK010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54261_length_1741_cov_4.259786"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLA17_11165"
     CDS             <1..538
                     /locus_tag="PLA17_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK86012.1"
                     /translation="VMAATATGQIPVTENAEGETPPVNQRLFFGGSFGLQFGTVTNIK
                     VAPLAGIWLLPRIAAGAGPTFQYYKDPFGRTSIYGGRAMLQLTLIQNLNNIIPIGLNA
                     GIYVNGEYEALSLQRAFFTSDPDAIGRFWHGTFLAGAGLSQPTGRRSSMNISFLWSIT
                     GNEYGIYDTPEIRIEFYF"
     gene            579..1115
                     /locus_tag="PLA17_11170"
     CDS             579..1115
                     /locus_tag="PLA17_11170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPK86013.1"
                     /translation="MKIKSSRFASLAYTLHEGDKGGRVIETVEESAPLSFVFGAGQLL
                     PAFEDKLAGLEAGDEFDFSLVADDAYGEVREEMVISLPRNIFEDEGVLRSEICYVGNT
                     VPMLDSHGNRMNGVVVEIGDAFIKMDFNHPLAGIDLHFSGKVVEVREATPGELEGPSS
                     GGCSSCSSRNSGCGGTCG"
     gene            complement(1276..>1741)
                     /locus_tag="PLA17_11175"
     CDS             complement(1276..>1741)
                     /locus_tag="PLA17_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPK86014.1"
                     /translation="NMSTVLLTAFSTSSSNATLPLTMETVERKNGVSSKISSFTLPLG
                     ATINMNGTALYECVAVMFIAQAYGIDLSLGQQLVVIFTALLAAIGSAGIPMAGLVMMA
                     VVLNAVGLPLEGIGLILAVDRILDMFRTAINVYGDTCGAVIIAKSEGEELLV"
BASE COUNT          437 a          402 c          497 g          405 t
ORIGIN      
        1 ggtgatggct gcaacggcga cgggacagat tccggtgacg gagaatgctg agggggagac
       61 gcctccggtt aatcagcgac tctttttcgg gggatcgttc ggcctgcagt ttggaacagt
      121 taccaatatc aaagtggccc cactggcggg gatatggctg ctaccaagga tcgctgccgg
      181 agcaggaccg acgttccagt attacaagga cccctttggc agaacctcca tctacggggg
      241 gagggcaatg ctgcaactga ctctcataca gaacctgaac aatattatcc cgataggcct
      301 gaatgccgga atctatgtca atggtgagta tgaggcacta agcctgcaac gggccttctt
      361 cacttccgac ccggacgcta ttggtcgctt ctggcatggc acttttctgg caggagcagg
      421 attgtcacag cctacaggca ggaggtcaag catgaacatc agtttcctct ggagcattac
      481 cggaaatgaa tatggaatct atgatactcc ggagatcaga atagagttct atttttaatt
      541 gatataacct aattgaatat aaatcagatc agatagttat gaaaatcaaa agcagcaggt
      601 ttgcctccct ggcatatacc cttcacgagg gtgataaggg gggaagggtg attgaaacgg
      661 tggaagagtc agcccctctt tcgtttgtct ttggagcggg ccaattgttg ccggcatttg
      721 aagataaact ggcgggactg gaagcgggag atgaatttga tttcagccta gtggctgatg
      781 acgcgtatgg tgaggtacgc gaggagatgg tgatcagcct tccaagaaat atatttgagg
      841 atgaaggtgt tctgagaagt gagatatgct atgtgggtaa cactgttccc atgctggact
      901 ctcatgggaa caggatgaat ggtgtggttg tggaaattgg cgatgctttt ataaagatgg
      961 atttcaatca tcccctggca ggcattgacc ttcatttttc aggcaaggtt gtagaggtgc
     1021 gcgaagcaac acccggggaa cttgagggtc cgtcatccgg cggctgctca tcatgcagca
     1081 gcaggaacag cggttgcggc ggcacttgcg gatgagaaac agtcagcggc aggtagtcgg
     1141 cagccagtgg cagtcagtca gtagtcagcg gcaggtagcc ggcagtcagt ggcagtcagt
     1201 cagtagtcac cggcacgtag ccgtcagcca gtcagttaca cgccagtcag ctacagccgg
     1261 gtgctgacag cacgattata ccaggagctc ttctccttcc gacttggcaa taatgaccgc
     1321 accgcaggta tctccgtaaa catttatggc agtgcggaac atgtccagaa tacggtctac
     1381 ggcaagtatc agacctatcc cttcaagcgg aagtcccaca gcattcagca ccacagccat
     1441 cattaccagt cctgccattg gtatccctgc cgagccgatg gctgcaagca gggctgtgaa
     1501 tattaccacc agttgttgcc cgagactcag gtcaataccg taagcctgtg ctataaacat
     1561 cacagccaca cattcgtaga gcgctgttcc gttcatgttg atggtggccc cgaggggcag
     1621 ggtgaagctt gaaatcttgc ttgagacacc attctttctc tcaacagtct ccatggtaag
     1681 aggaagcgtg gcgttggatg aggatgtgga gaaagctgtc agaagaacag ttgacatgtt
     1741 c
//
LOCUS       DAOPBK010000279         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_57013_length_1685_cov_6.007362, whole genome shotgun sequence.
ACCESSION   DAOPBK010000279 DAOPBK010000000
VERSION     DAOPBK010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57013_length_1685_cov_6.007362"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /gene="nadE"
                     /locus_tag="PLA17_11180"
     CDS             <1..989
                     /gene="nadE"
                     /locus_tag="PLA17_11180"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPK86015.1"
                     /translation="IFDGSSVLIDSDGTIIDRLGFCTEEVRTVEIPSPGTDRNSLSFP
                     APLPGDMTGLIHQALVTGLRDFFVKGGIKRAIIGLSGGIDSAVVTALAAEALGPENTL
                     ALLMPSVYSSEHSVSDSVKMADKIGMPYHIVSIEDARQTVEKTLQPFFAGKEPGVTLE
                     NIQARLRAVILMAFANEFGYMLLNTSNKSEAAVGYGTLYGDMAGGLSVIGDLYKSEVY
                     QLAREINSHAEIIPPAIISKPPSAELKPDQLDTDSLPPYSLLDPVLYRHIDLEWSADR
                     IIADGFDKELVEEILRLVRTSEFKRKQTPPVLRVSSKAFGTGRRIPIIATRH"
     gene            1101..>1685
                     /locus_tag="PLA17_11185"
     CDS             1101..>1685
                     /locus_tag="PLA17_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPK86016.1"
                     /translation="MLYTSPYLEQCIEGPFSVFRDLPDKEKEFLLQNHSCISLKKGEM
                     IFHEGNRAAGVYCLASGKVKVYKEGIGGREQIIRMVRPQGLIGFAAFISGNAYSASAV
                     AIEESGVCHFDKEPFMKLIRRNPDLSMKLMRMMAIELSFSNGRTISLTQKHIRGRLAE
                     SLLVLRDTYGVENDGRTIKAYVSREDLACFSNMTT"
BASE COUNT          431 a          416 c          425 g          413 t
ORIGIN      
        1 tcatctttga cggttcctcc gttctcattg attctgacgg aaccatcatt gacagactgg
       61 gattctgcac cgaggaggtc aggacagtgg agataccgtc accgggaact gaccggaaca
      121 gcctctcctt ccctgccccg cttcccgggg atatgacagg cctcattcac caggcgctgg
      181 ttacggggct acgcgatttc ttcgtgaaag gcgggataaa aagagccatc atcggacttt
      241 ccggtggaat tgactcagcg gtggtaacgg cacttgcagc agaagccctg gggccagaga
      301 acaccctcgc cctgctgatg ccctcagtct actcatcaga acactcggtg tcagactcgg
      361 tgaagatggc agataagatc gggatgcctt accatatagt gagcatagag gatgcacgcc
      421 agacagtgga aaaaaccctt cagcccttct tcgccggaaa ggaacctggt gttaccctgg
      481 aaaacattca ggcaaggctc cgcgctgtga tactaatggc tttcgcaaat gaattcggat
      541 atatgcttct caatacctcc aacaagagcg aagctgctgt aggttacgga acactgtacg
      601 gtgatatggc aggaggcctc tctgtaatag gtgacctcta caagagcgaa gtgtaccagc
      661 ttgccaggga gataaacagc catgcggaga tcatacctcc tgcaataata tccaaacctc
      721 cgtcagcgga gcttaaacct gaccagctcg ataccgattc ccttcctccc tacagcctgc
      781 tcgatccggt cctgtaccgg catattgacc tggagtggtc cgctgaccgg attattgctg
      841 acggatttga caaggagctg gttgaggaga tcctcagact cgtccgcacc agcgaattca
      901 agagaaaaca aaccccgccg gtactccggg tctcatcaaa agccttcggc acaggaagaa
      961 ggataccaat tatagccaca cgccactgat actattacct gacactcaac tatctgagta
     1021 gtagtgtgtt aaaaaacgtt aattttccct tttgagcact tttttaatat ctttgtatat
     1081 gttgttaaac ataaggggaa atgctttata ccagtccgta ccttgagcaa tgcattgaag
     1141 gaccgttttc tgttttcagg gatcttcctg acaaggagaa ggagtttctg ttacagaatc
     1201 attcatgcat ttcgttgaaa aagggggaaa tgatttttca cgagggtaac agggcggcag
     1261 gtgtttactg tctggcatca ggtaaggtca aggtgtacaa ggaaggcatc gggggtcgtg
     1321 aacagattat aagaatggta aggccgcagg gtctgatagg ttttgctgct tttatttccg
     1381 gcaacgccta ttccgcttca gcggtagcta tagaagaatc gggtgtctgc cactttgaca
     1441 aggagccgtt tatgaagctg atcagacgca atcctgatct gtcgatgaag ctcatgagaa
     1501 tgatggcaat tgaactctcg ttctctaacg ggagaaccat ctcgctgacc cagaaacata
     1561 tccgaggtcg ccttgccgag tcgctgcttg tcctgcgtga tacctacggt gttgagaatg
     1621 acggccgtac tatcaaggcc tatgtctcgc gtgaagacct tgcctgcttt tcaaacatga
     1681 ctacc
//
LOCUS       DAOPBK010000280         1641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_59315_length_1641_cov_3.542875, whole genome shotgun sequence.
ACCESSION   DAOPBK010000280 DAOPBK010000000
VERSION     DAOPBK010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59315_length_1641_cov_3.542875"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLA17_11190"
     CDS             <1..244
                     /locus_tag="PLA17_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK86017.1"
                     /translation="VTYTISKDNTWEVAVYDFDGVPGGFDWTGDMYAPNVVADDRFNH
                     DYYNTLRIMCNQTVADGTHSFWLDELSGPHVEGIHK"
     gene            324..>1641
                     /locus_tag="PLA17_11195"
     CDS             324..>1641
                     /locus_tag="PLA17_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPK86018.1"
                     /translation="MKLTRLSLVLSAGLFMLIAGSCDRGKSAADSPESRADRLVKQMT
                     LDEKIGQMSQLDLGYFMDPEQLKQAVREGRCGSLLNFLGTEQVNELQRIAVEESRLGI
                     PLIIGRDVIHGYRTVFPIPLGMAASWNPEAVRQAFRISAVEASTDGIRWTFAPMIDIT
                     WDPRWGRIAEGCGEDPYLASTLAAAMVRGIQGDSLTDPTALAACAKHYVGYGFAEAGR
                     DYNTTYIPEPLLRNVALKPFHAAKEAGAQTFMSAFNDLNGIPTSGNQFTLKKILREEW
                     GFDGFVVSDWASVTEMINHGYAEDEKHAAEIAINAGVDMEMATTSYYTNIKALLDERK
                     ITMAQIDDAVRNILKVKFRLGLFDNPYTDPGAGKKILDPSFLDHARTVARQSAVLLKN
                     DNAALPLSDEKISSVAVIGPLADAPRDQLGTWCFDGREENSVTPLKA"
BASE COUNT          427 a          374 c          441 g          399 t
ORIGIN      
        1 ggtaacatat acgatcagta aagacaacac ctgggaagtt gcagtgtatg acttcgacgg
       61 tgtacccgga gggtttgact ggacaggaga catgtatgcc ccgaacgtgg ttgctgatga
      121 caggttcaat catgattatt acaacacatt acgtattatg tgcaatcaga cggttgccga
      181 cggtactcac agtttctggc tggatgagct gtcaggcccg cacgtggagg gtatccataa
      241 ataatatttt ttgtttataa ttgcctgccc tgaagaaggg caggcaatga atttcatttt
      301 tataaacctt tcagagaaga cagatgaaac tcacgagact atcgctggtt ttgtcagcag
      361 gactttttat gctgattgcc ggctcatgtg acaggggaaa aagtgctgct gacagtcctg
      421 agtccagggc tgacaggctg gttaagcaga tgaccctgga cgaaaagatc gggcagatgt
      481 cacagcttga ccttggctat ttcatggacc ctgaacaatt aaaacaggct gtcagggaag
      541 gaagatgcgg ttcgctgttg aattttcttg gcactgagca ggtgaatgag ctacaacgca
      601 tagctgtaga ggagagcagg ctcggaattc cgctgatcat cggaagggat gttattcacg
      661 gttaccgtac ggtattcccc attccacttg ggatggcagc atcatggaac cctgaagctg
      721 tcaggcaggc attccgcatc tctgccgtcg aggcttcaac cgatggtata cgatggacct
      781 tcgccccgat gattgacatt acatgggatc cccgctgggg caggattgcc gagggatgcg
      841 gtgaggatcc atatcttgca tcaacccttg cagccgcaat ggtaagaggg attcagggcg
      901 acagcctcac tgatcccact gcgctggcag catgcgcaaa acactatgtt gggtacggtt
      961 tcgctgaagc cggacgtgat tacaatacca cctatatccc tgaaccactt ctccggaatg
     1021 tggctcttaa accttttcat gctgcaaagg aggccggtgc acagaccttc atgtctgcat
     1081 tcaatgacct gaacggtatc cctacatcag gcaaccagtt tactctgaaa aagatactta
     1141 gggaagagtg gggctttgac ggtttcgtgg tgagtgactg ggcttctgtc accgagatga
     1201 ttaaccacgg atacgcagag gatgaaaagc atgctgcaga gatagcaatc aatgcaggtg
     1261 ttgatatgga aatggctaca acatcatact acaccaacat aaaggctctg cttgatgagc
     1321 ggaagataac catggcgcag attgacgatg ctgtaaggaa tatactcaag gtaaaattca
     1381 ggcttgggct gtttgacaat ccatatactg atcccggagc cgggaagaag atacttgacc
     1441 cttcatttct tgatcatgcc cgcactgtag caaggcagag cgcagttctg ctcaagaacg
     1501 ataatgctgc actgcctctc tctgacgaga agatctcttc cgtggctgtg atcggtcctc
     1561 ttgctgatgc gccgcgtgac cagcttggaa catggtgctt tgacggcagg gaggagaatt
     1621 ccgtgacccc gctgaaagca a
//
LOCUS       DAOPBK010000281         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_60698_length_1615_cov_5.364103, whole genome shotgun sequence.
ACCESSION   DAOPBK010000281 DAOPBK010000000
VERSION     DAOPBK010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60698_length_1615_cov_5.364103"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1435
                     /locus_tag="PLA17_11200"
     CDS             137..1435
                     /locus_tag="PLA17_11200"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPK86019.1"
                     /translation="MRFRFVPAALVAAAMLTSLSCVRSGAAENTPPGDVARPVSSETP
                     SDTAFVSGLPADSTAMDSGQNTLFGIPVDSYNVKTEKVRRNQFISSILAAQGISWNDI
                     EKLLSDNKETFDPRRVRSGSSYSVLTTKDTLSKAEYIIYQHDPRVSYVFSFKDTLAIY
                     RHDAEIRRLLRYSSGTISTSLWEATLEQNLNPNLSAELSEIYAWTIDFFGLQKGDRFK
                     VIYEEDFIGDESVGISRVHAAEFEHAGSTIYAIPYIQDSVMSFYDTTGASLRKAFLKA
                     PLRFSRISSRFSGARMHPILKIVRPHHGVDYAAPVGTPVLAIGDGRVTSTAYEGGSGR
                     IVRITHNSVYSTAYMHLSRFGPGISPGVYVRQGQVIGYVGSSGLSTGPHLDFRFYRNG
                     SPIDPLKVEAPPVNPVAPESMDEFNRVAEAYIKLLATIKY"
BASE COUNT          358 a          395 c          457 g          405 t
ORIGIN      
        1 agatagagtt gcctgtcgac ctccccgggg aggaggtgaa aaagatcgtg ctcgctgatg
       61 agagggcccg gaaatggatt ggccaggcaa caccaaaagt tatagtagta ccaaaccgta
      121 ttgtaaacat cgttgtatga ggttcaggtt tgttcctgcg gccctggttg ctgccgcaat
      181 gctgacatca ctttcctgcg ttcgcagcgg agctgctgaa aatacacctc cgggagatgt
      241 ggccagaccg gtctcttcag aaacaccctc tgatactgct ttcgtgtcag gtttgcctgc
      301 cgattcaacg gcaatggatt caggtcagaa tacccttttc gggatacccg ttgactcata
      361 caatgttaaa acggagaagg tccgaaggaa ccagttcatc tcttcaatcc tggcagccca
      421 gggtatcagc tggaatgaca ttgaaaagct gctcagtgac aataaagaga cttttgatcc
      481 gcgcagggtg cggagtggca gttcctattc ggtactgaca acaaaagaca cactcagcaa
      541 agcggaatac attatttatc agcatgatcc gagggtgtca tatgttttct ccttcaagga
      601 caccctggcc atatacaggc atgatgcaga gataagacga ttattgcggt actcatccgg
      661 aacaatttcc acctcgctgt gggaggctac tctggagcag aacctcaatc ccaacctctc
      721 ggctgagctg tcagagatct atgcatggac catcgacttc ttcggactgc agaagggtga
      781 ccggttcaag gtgatttacg aggaggattt cattggtgat gagtcggttg gcatcagccg
      841 tgttcatgca gcagaattcg agcatgccgg gagtacgatc tatgccattc cctatattca
      901 ggacagtgtc atgtcatttt atgacaccac cggagccagt ctgcgcaagg cttttctcaa
      961 ggcgccgctg aggttctcgc gcatcagctc ccgcttttcg ggcgccagga tgcatccgat
     1021 actgaagatt gtaaggcccc atcacggcgt tgactacgca gcgcctgtcg gtacgccggt
     1081 gttggccata ggcgacggga gggtgacaag cacagcctat gagggtggtt caggaaggat
     1141 tgtacgcata actcacaaca gtgtttactc gaccgcatac atgcatctct cccggtttgg
     1201 cccgggcatc tcaccaggag tatatgtcag gcaggggcag gtgataggtt acgtggggag
     1261 ctccggcctc tccacaggac ctcatcttga tttcaggttc taccggaacg gatcgcccat
     1321 tgatccgttg aaggttgagg cgcctcccgt caatcctgtt gccccggaat caatggatga
     1381 gttcaacagg gtggctgagg cgtatatcaa attgcttgct actataaaat attagggttc
     1441 tgtctggtgg ccgggcaggg tttctgccag ttagtcgggc tgagtttgcg tccgtgactc
     1501 tggctgagtt tctgcctggt ggccgggcga gggtttgtgt ccgtgactct ggctgagtct
     1561 atgtctggtg agcgggttgg gttctcctta ttggtctggg cagggttctg cctgt
//
LOCUS       DAOPBK010000282         1548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_64537_length_1548_cov_5.645680, whole genome shotgun sequence.
ACCESSION   DAOPBK010000282 DAOPBK010000000
VERSION     DAOPBK010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64537_length_1548_cov_5.645680"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLA17_11205"
     CDS             <1..453
                     /locus_tag="PLA17_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HPK86020.1"
                     /translation="TAYEVFTGKKLSRIDFTELRGMEGVRQATIDVDGFPLNIGIAHG
                     LGNARKLLDDVKAGRSKFHAIEVMACPGGCVGGGGQPLHHGDSAILKARAKALYDADR
                     DMPIRLSHENPYIIQLYKEYLGEPNSEKAHHLLHTHYFDRKAKIVVKQ"
     gene            484..969
                     /gene="nuoE"
                     /locus_tag="PLA17_11210"
     CDS             484..969
                     /gene="nuoE"
                     /locus_tag="PLA17_11210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPK86021.1"
                     /translation="MSTTIKIELRQQDLKKIHEICDSFNNEPLELINILHKTQEHFGY
                     LPAEIQDAVADKLNISTAQVYGVVTFYSFFTMTPKGKHPISICMGTACYVRGAERILD
                     EFKKELGLQVGQTTPDGKFSLSSLRCVGACGLAPVVIVGNKTYGRVAPDDVKAILKEY
                     E"
     gene            complement(1068..>1548)
                     /locus_tag="PLA17_11215"
     CDS             complement(1068..>1548)
                     /locus_tag="PLA17_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPK86022.1"
                     /translation="LIKDYNKAAGNAEEQYLRELVMNHTSGRLKVAPEHTDDEVLYLM
                     RKSSFRHFRKFREKFISITRAGGFRYELIPYFISAHPGTSLKKMEALSRELSDLGMHP
                     EQVQDFTPTPMTLSTAIFYLGFDPYTGKKVFVERDIEGKRKQKGYFFKGKTTKRHQY"
BASE COUNT          431 a          382 c          367 g          368 t
ORIGIN      
        1 acagcatacg aagtctttac aggcaagaaa ctcagccgta tcgattttac cgaactgaga
       61 ggaatggaag gagtacgtca ggctacgata gatgttgacg gcttcccgct taacataggt
      121 atcgctcacg gtctgggcaa cgcccgcaaa ctccttgacg atgtgaaggc aggtcgctca
      181 aaattccatg ccatagaagt gatggcatgc ccgggcggct gcgtcggcgg tggcggccaa
      241 ccactgcatc acggtgactc cgcaatactc aaggcaagag ctaaggcact ctacgatgca
      301 gaccgtgaca tgccaataag gttatctcac gaaaacccct acattatcca gctttacaag
      361 gagtatcttg gcgaacctaa cagcgaaaag gctcaccacc tgctgcatac acactatttt
      421 gacagaaagg caaagatcgt tgtcaaacag taaacgcggt aattaacatt aaaaatcaaa
      481 gagatgtcaa caactataaa gatagaactg agacagcaag atctgaaaaa gatccatgag
      541 atctgcgatt ctttcaacaa tgaaccgctg gagctgataa acatccttca caaaacacag
      601 gaacatttcg gctatctgcc tgcagagata caggacgctg ttgccgataa actgaatatc
      661 tccacagcac aggtttacgg cgtcgtgacc ttctattcat tctttaccat gaccccaaaa
      721 ggcaagcacc cgataagcat ctgcatgggt actgcctgct acgtcagagg tgcagaaagg
      781 atcctcgatg agtttaagaa agaactgggc ctgcaggttg gacaaacaac ccctgacgga
      841 aagttctctc tctcaagcct tcgttgcgtg ggcgcatgcg gactggcacc ggtggtgata
      901 gtcggtaaca aaacctacgg aagggtcgct cccgatgatg tgaaagcaat attgaaagaa
      961 tacgaatagt aaaaaagggc cggcaatacc ggcctttttt ttcaggcatc agccattggg
     1021 cagcagtgac acttccacga taacagactg tcagtaatga ttcattgtca atactggtgt
     1081 cttttggttg ttttaccctt gaaaaagtac cccttctgct tcctctttcc ctctatatca
     1141 cgctctacga agaccttttt ccccgtatag ggatcaaatc cgaggtagaa tatggcagtg
     1201 gagagtgtca tgggagtagg ggtaaaatcc tgcacctgct ccggatgcat ccccaagtca
     1261 gagagctcac gggagagagc ctccattttc ttcaggctgg tgccgggatg ggctgagata
     1321 aagtagggta tgagctcata cctgaacccg ccggctctgg ttattgagat gaacttttcc
     1381 ctgaacttcc tgaagtgcct gaaagagctc tttctcatca ggtaaagtac ctcatcatcc
     1441 gtatgttcag gggcaacttt gagcctgcca ctggtgtggt tcatcaccag ttcccgcaga
     1501 tattgctctt cagcatttcc ggcagccttg ttgtaatcct ttatcagc
//
LOCUS       DAOPBK010000283         1523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3487
            NODE_66118_length_1523_cov_5.152589, whole genome shotgun sequence.
ACCESSION   DAOPBK010000283 DAOPBK010000000
VERSION     DAOPBK010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865222
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:32:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,246
            CDSs (total)                      :: 2,214
            Genes (coding)                    :: 2,204
            CDSs (with protein)               :: 2,204
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66118_length_1523_cov_5.152589"
                     /isolate="AMR_MDS_3487"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLA17_11220"
     CDS             complement(<1..525)
                     /locus_tag="PLA17_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK86023.1"
                     /translation="MQSAESLKRKIEMEQRNESIRVSPSGTATIKIGDDSNSSEILVF
                     TTLCNVYKVQKKELTDKVQALHKIDTEPGERPVYLTGDKRYKGFLIVAFENGKIGKIS
                     MESYKTEYTRKKLRSAFNGESRLVFIELIERDIDLVAMSSIKKVILFNTSLINAVESR
                     TTKGVQVMKPKDGSV"
     gene            complement(630..1142)
                     /locus_tag="PLA17_11225"
     CDS             complement(630..1142)
                     /locus_tag="PLA17_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPK86024.1"
                     /translation="MIKLLLGDITKIGVDAIVNAANSSLLGGGGVDGAIHRAGGRAIL
                     DECRKIVERQGGCPTGEAVITTAGNMPARYVIHTVGPVWHGGMNNEDQLLANAYSNSL
                     RLAVDNGVKTIAFPNISTGVYGFPKERAANIAIEAVRKFLKNDKSLHEVVFVCFDREN
                     HAIYERLLNV"
     gene            complement(1144..1455)
                     /locus_tag="PLA17_11230"
     CDS             complement(1144..1455)
                     /locus_tag="PLA17_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK86025.1"
                     /translation="MYITNMLHFLDEKGNIHAEMPRESREMAAFLALVVDASTGMMSH
                     EYNATGIRCFEEGCTENVVVRLSDVRDEIEWLCPECHNEGRISHWQGTRRDNSATIKK
                     R"
BASE COUNT          373 a          408 c          284 g          458 t
ORIGIN      
        1 gacgctgcca tccttgggct tcataacctg aacgcccttc gttgtccggc tttcaaccgc
       61 attaatcaga gaggtgttga acaggataac ctttttaatg ctgctcatgg caaccagatc
      121 aatatcacgc tcgatgagct caataaaaac cagtcgcgac tcgccattga aggcactcct
      181 gagcttcttc ctcgtgtact cggttttata gctctccatc gatatcttcc cgattttacc
      241 attttcaaat gcgactatca gaaagccttt atatcttttg tcaccggtca gataaacagg
      301 tctctcaccc ggttcagtat caatcttatg aagcgcctgc accttgtcag taagctcctt
      361 cttctgaacc ttgtaaacat tgcaaagggt ggtaaagaca agtatctcac ttgaatttga
      421 atcgtccccg atttttattg ttgctgttcc cgacggagat acccttatcg attcattcct
      481 ctgttccatc tctattttcc ttttcaggct ttctgcactt tgcatggtaa tcaggttttg
      541 ttttgaatcc aaatgtggga attattcgtt ttgcatctgc aaaactcaat tatgtttttc
      601 gcagtaatag ttagcagtga actctctgtt cagacattca gcagcctctc atagattgca
      661 tggttctcac ggtcaaaaca gacgaacacc acttcatgca gggatttgtc attcttcaaa
      721 aacttcctga cagcttcgat agcaatgttc gctgctctct ctttcgggaa gccgtagact
      781 ccggtgctga tattgggaaa tgcaatagtt tttactccgt tatccaccgc aagacgcaat
      841 gagttggaat atgcatttgc aagcagctga tcttcattgt tcattccgcc gtgccatacc
      901 ggacccactg tatgaatgac atacctggcc ggcatattcc cggccgtggt tatcaccgcc
      961 tcaccagtgg ggcaaccgcc ctgtctctca acaatcttcc tgcattcgtc aaggatagcc
     1021 ctgcctcctg ccctgtggat agccccgtca acgccaccac ctccgagaag cgatgaattt
     1081 gcagcgttga caattgcatc aaccccgatc tttgtaatgt ctccaagtag tagttttatc
     1141 attttaccgt ttctttattg tcgctgaatt atcccgcctt gtaccctgcc agtgactgat
     1201 tctcccctca ttatgacatt ccgggcaaag ccattctatt tcatccctga catctgacaa
     1261 cctgaccacc acgttttcag tacatccttc ttcgaagcat ctgatgccgg tagcattgta
     1321 ttcatgactc atcattccgg tagaggcatc aaccaccagc gcaagaaaag cagccatctc
     1381 tctggactcg cggggcatct ccgcatgaat atttcccttt tcgtcaagga aatgaagcat
     1441 gttggttata tacatacact tatccgcttg aaaacaaaac tgcaagtctg gtcactgcat
     1501 cattacaaaa atacacaatg aaa
//
